METALLOPROTEASE INHIBITORS

Abstract: Compounds of formula (I) and pharmaceutically-acceptable derivatives thereof, are matrix metalloprotease inhibitors, useful in treatment of conditions mediated by matrix metalloproteases, such as chronic dermal ulcers.

Description: METALLOPROTEASE INHIBITORS This invention relates to a series of substituted α-aminosulphonyl-acetohydroxamic acids which are inhibitors of zinc-dependent metalloprotease enzymes. In particular, the compounds are inhibitors of certain members of the matrix metalloprotease (MMP) family. Matrix metalloproteases (MMPs) constitute a family of structurally similar zinc-containing metalloproteases, which are involved in the remodelling and degradation of extracellular matrix proteins, both as part of normal physiological processes and in pathological conditions. Since they have high destructive potential, MMPs are usually under close regulation and failure to maintain MMP regulation has been implicated as a component of a number of diseases and conditions including pathological conditions, such as atherosclerotic plaque rupture, heart failure, restenosis, periodontal disease, tissue ulceration, cancer metastasis, tumour angiogenesis, age-related macular degeneration, fibrotic disease, rheumatoid arthritis, osteoarthritis and inflammatory diseases dependent on migratory inflammatory cells. Another important function of certain MMPs is to activate various enzymes, including other MMPs, by cleaving the pro-domains from their protease domains. Thus some MMPs act to regulate the activities of other MMPs, so that over-production of one MMP may lead to excessive proteolysis of extracellular matrix by another. Moreover, MMPs have different substrate preferences (shown in the following Table for selected family members) and different functions within normal and pathological conditions. For recent reviews of MMPs, see Current Pharmaceutical Design, 1996, 2, 624 and Exp. Opin. Ther. Patents, 1996, 6, 1305. TABLE Excessive production of MMP-3 is thought to be responsible for pathological tissue breakdown which underlies a number of diseases and conditions. For example, MMP-3 has been found in the synovium and cartilage of osteoarthritis and rheumatoid arthritis patients, thus implicating MMP-3 m the joint damage caused by these diseases: see Biochemistry, 1989, 28, 8691 and Biochem J , 1989, 258. 115 MMP- 13 is also thought to play an important role in the pathology of osteoarthritis and rheumatoid arthritis see Lab Invest., 1997, 76, 717 and Arthritis Rheum., 1997, 40, 1391. The over-expression of MMP-3 has also been implicated in the tissue damage and chromcity of chronic wounds, such as venous ulcers, diabetic ulcers and pressure sores: see Brit. J. Dermatology, 1996, 135, 52. Collagenase-3 (MMP-13) has also recently been implicated in the pathology of chronic wounds (J Invest Dermatol , 1997, 109, 96-101). Furthermore, the production of MMP-3 may also cause tissue damage in conditions where there is ulceration of the colon (as in ulcerative colitis and Crohn's disease- see J. Immunol., 1997 158. 1582 and J. Clin Pathol., 1994, 47, 113) or of the duodenum (see Am J. Pathol., 1996, 148, 519). Moreover, MMP-3 is also thought to be mvolved in skm diseases such as dystrophic epidermolysis bullosa (see Arch. Dermatol. Res., 1995, 287, 428) and dermatitis herpetiformis (see J. Invest. Dermatology, 1995, 105, 184). Rupture of atherosclerotic plaques by MMP-3 has also been descπbed (see e.g. Circulation, 1997, 96, 396). Thus, MMP-3 inhibitors may find utility in the treatment of conditions caused by or complicated by embohc phenomena such as cardiac or cerebral infarctions. Studies of human cancers have shown that MMP-2 is activated on the invasive tumour cell surface (see J. Biol.Chem., 1993, 268. 14033) and BB-94, a non-selective peptidic hydroxamate MMP inhibitor, has been reported to decrease the tumour burden and prolong the survival of mice carrying human ovarian carcinoma xenografts (see Cancer Res., 1993, 53, 2087). Various seπes of MMP inhibitors have appeared in the literature which have a carbonyl moiety (CO) and a sulphone moiety (S0 2 ) with a two atom "spacer" interposed between them. For example, α- arylsulphonamido-substituted acetohydroxamic acids are disclosed in EP-A-0606046, WO-A-9627583 and WO-A-9719068, whilst EP-A-0780386 discloses certain related sulphone-substituted hydroxamic acids. The compounds of the present mvention represent a new class of compounds, and are inhibitors of some of the members of the MMP family. In particular, they are inhibitors of MMP-3 and/or MMP-13^ with certain compounds exhibiting varying degrees of selectivity over other MMPs, such as MMP-1, MMP-2, MMP-9 and MMP-14. Thus they may be of utility in treating diseases and conditions mediated by MMPs, in particular MMP-3 and/or MMP-13. A series of substances related to the instant mvention were disclosed in International Patent Application number publication no. WO 99/29667, herein incorporated by reference m its entirety. According to one aspect of the present invention ("A"), there is provided a compound of formula (I): (I) and pharmaceutically-acceptable salts thereof, and solvates thereof, wherein the dotted line represents an optional bond, X is a monocyclic aromatic linker moiety selected from phenylene, pyridinylene, pyrazolylene, thiazolylene, thienylene, furylene, pyrimidinylene, pyrazinylene, pyridazinylene, pyrrolylene, oxazolylene, isoxazolylene, oxadiazolylene, thiadiazolylene, imidazolylene, triazolylene, or tetrazolylene; R is H, C alkyl optionally substituted by C, ^ alkoxy, NR 4 R 5 or OH, or R is C alkoxy optionally substituted by 1 or 2 substituents selected from (C alkyl optionally substituted by OH), C, ^ , alkoxy, OH and NR 4 R 5 ; R 1 and R 2 are each independently H, C lJ5 alkyl optionally substituted by OH or C alkoxy, or C^ alkenyl; or R 1 and R 2 are taken together, with the C atom to which they are attached, to form a 3- to 7-membered ring optionally incorporating a hetero- moiety selected from O, S, SO, S0 2 and NR 6 , and which 3- to 7- membered ring is optionally substituted by one or more OH; R 3 is H, halo, methyl, or methoxy; R 4 and R 5 are each independently H or C, to C 6 alkyl optionally substituted by OH, C, to C 4 alkoxy or aryl, or R 4 and R 5 can be taken together with the N atom to which they are attached , to form a 3- to 7- membered ring, optionally incorporating a further hetero- moiety selected from O, S, S0 2 and NR 7 ; and R 6 and R 7 are each independently H or C, to C 4 alkyl. According to a further aspect of the invention ("B"), there is provided a compound of formula (I): (I) and pharmaceutically-acceptable salts thereof, and solvates thereof, wherein the dotted line represents an optional bond; X is a monocyclic aromatic linker moiety selected from pyrazolylene, thiazolylene, pyrazinylene, pyridazinylene, pyrrolylene, oxazolylene, isoxazolylene, oxadiazolylene, thiadiazolylene, imidazolylene, triazolylene, or tetrazolylene; R is H, C alkyl optionally substituted by C alkoxy or NR 4 R 5 or OH, or C alkoxy optionally substituted by 1 or 2 substituents selected from (C alkyl optionally substituted by OH), C alkoxy, OH and NR R 5 ; R 1 and R 2 are each independently H, C,^ alkyl optionally substituted by OH or C alkoxy, or C 7 __ alkenyl; or R 1 and R 2 are taken, together with the C atom to which they are attached, to form a 3- to 7-membered ring optionally incorporating a hetero- moiety selected from O, S, SO, S0 2 and NR 6 , and which 3- to 7- membered ring is optionally substituted by one or more OH; R 3 is H, halo, methyl, or methoxy; R 4 and R 5 are each independently H or C, to C 6 alkyl optionally substituted by OH, C, to C 4 alkoxy or aryl, or R 4 and R 5 can be taken together with the N atom to which they are attached , to form a 3- to 7- membered ring, optionally incorporating a further hetero- moiety selected from O, S, S0 2 and NR 7 ; and R δ and R 7 are each independently H or C, to C 4 alkyl. According to a further aspect of the invention ("C") there is provided a compound of formula (I): WO 00/74681 PCT/IB0O/OO667 (I) and pharmaceutically-acceptable salts thereof, and solvates thereof, wherein the dotted line represents an optional bond; X is a monocyclic aromatic linker moiety selected from phenylene, pyridinylene, pyrazolylene, thiazolylene, thienylene, furylene, pyrimidinylene, pyrazinylene, pyridazinylene, pyrrolylene, oxazolylene, isoxazolylene, oxadiazolylene, thiadiazolylene, imidazolylene, triazolylene, or tetrazolylene; R is C,^ alkyl substituted by NR 4 R 5 , C alkoxy substituted by NR 4 R 5 , or C alkoxy substituted by 2 substituents selected from (C alkyl optionally substituted by OH), C alkoxy, OH and NR R 5 ; R 1 and R 2 are each independently H, C,^ alkyl optionally substituted by OH or C alkoxy, or C 2 __ alkenyl; or R 1 and R 2 are taken together, with the C atom to which they are attached, to form a 3- to 7-membered ring optionally incorporating a hetero- moiety selected from O, S, SO, S0 2 and NR 6 , and which 3- to 7- membered ring is optionally substituted by one or more OH; R 3 is H, halo, methyl, or methoxy; R 4 and R 5 are each independently H or C, to C 6 alkyl optionally substituted by OH, C, to C 4 alkoxy or aryl, or R 4 and R 5 can be taken together with the N atom to which they are attached , to form a 3- to 7- membered ring, optionally incorporating a further hetero- moiety selected from O, S, S0 2 and NR 7 ; and R 6 and R 7 are each independently H or C, to C 4 alkyl. According to a further aspect of the invention ("D") there is provided a compound of formula (I): (I) and pharmaceutically-acceptable salts thereof, and solvates thereof, wherein the dotted line represents an optional bond, X is a monocyclic aromatic linker moiety selected from phenylene, pyridinylene, pyrazolylene, thiazolylene, thienylene, furylene, pyrimidinylene, pyrazinylene, pyridazinylene, pyrrolylene, oxazolylene, isoxazolylene, oxadiazolylene, thiadiazolylene, imidazolylene, triazolylene, or tetrazolylene; R is H, C alkyl optionally substituted by C,^ alkoxy, NR 4 R 5 or OH, or C alkoxy optionally substituted by 1 or 2 substituents selected from (C alkyl optionally substituted by OH), C alkoxy, OH and NR R 5 ; R' and R 2 are each independently C,^ alkyl substituted by OH; or R 1 and R 2 are taken together, with the C atom to which they are attached, to form a 3- to 7-membered ring optionally incorporating a hetero- moiety selected from O, S, SO, S0 2 and NR 6 , and which 3- to 7- membered ring is substituted by one or more OH; R 3 is H, halo, methyl, or methoxy; R 4 and R 5 are each independently H or C, to C 6 alkyl optionally substituted by OH, C, to C„ alkoxy or aryl, or R 4 and R 5 can be taken together with the N atom to which they are attached , to form a 3- to 7- membered ring, optionally incorporating a further hetero- moiety selected from O, S, S0 2 and NR 7 ; and R 6 and R 7 are each independently H or C, to C 4 alkyl. In all the above definitions A, B, C and D, unless otherwise indicated, alkyl, alkenyl, alkoxy, etc. groups having three or more carbon atoms may be straight chain or branched chain. All the compounds of formula (I) in aspects A, B, C and D above may contain one or more chiral centres and therefore can exist as stereoisomers, i.e. as enantiomers or diastereoisomers, as well as mixtures thereof. The invention includes both the individual stereoisomers of the compounds of formula (I) and any mixture thereof. Separation of diastereoisomers may be achieved by conventional techniques, e.g. by fractional crystallisation or chromatography (including HPLC) of a diastereoisomeric mixture of a compound of formula (I) or a suitable salt or derivative thereof. An individual enantiomer of a compound of formula (I) may be prepared from a corresponding optically pure intermediate or by resolution, either by HPLC of the racemate using a suitable chiral support or, where appropriate, by fractional crystallisation of the diastereoisomeric salts formed by reaction of the racemate with a suitable optically active base or acid, as appropriate to the specific compound to be resolved. Furthermore, compound of formula (I) which contain alkenyl groups can exist as cis- or trans- geometric isomers. Again, the invention includes both the separated individual geometric isomers as well as mixtures thereof. Certain of the compounds of formula (I) may be tautomeric and all possible tautomers are included in the scope of this invention. Certain of the compounds of formula (I) may exhibit zwitterionic behaviour and all possible zwitterions are included in the scope of this invention. Also included in the invention are radiolabelled derivatives of compounds of formula (I) which are suitable for biological studies. WO 00/74681 7 PCT/IBO0/OO667 The pharmaceutically acceptable salts of all the compounds of the formula (I) include the acid addition and the base salts thereof The term "pharmaceutically acceptable" means suitable for use in human or non-human animal medicine Suitable acid addition salts are formed from acids which form non-toxic salts and examples include the hydrochloride, hydrobromide, hydroiodide, sulphate, hydrogen sulphate, nitrate, phosphate, hydrogen phosphate, acetate, maleate, fumarate, lactate, tartrate, citrate, gluconate, succinate, benzoate, methanesulphonate, benzenesulphonate and p-toluenesulphonate salts Suitable base salts are formed from bases which form non-toxic salts and examples mclude the aluminium, calcium, lithium, magnesium, potassium, sodium, zmc, tπs, meglumine, choline, olamine, diolamine, ethylenediamme, benetharmne, benzathene, glucosamine, mcotinamide, ornithine, guanidine, guanine, arginine and procaine salts For a review on suitable salts see for example Berge et al. J Pharm Sci , 66, 1-19 (1977) Solvates (e g hydrates) of the compounds and salts of aspects A, B, C and D of the mvention are mcluded in the mvention In some cases, the solvate may be the direct product of a reaction to make a compound or salt of the mvention m a solvent, m which case no further transformation step would be necessary In other cases, solvates may be made by methods known in the art, such as by crystallisation from a solvent Prodrugs of the compounds of aspects A, B, C and D of the invention, their pharmaceutically acceptable salts and solvates thereof, are also envisaged by the invention For reference as to how to prepare prodrugs, see standard texts in this field, for example "Design of Prodrugs" ed H Bundgaard (1985, Elsevier, Amsterdam / New York / Oxford) For aspects C and D of the mvention, X is preferably phenylene, pyπdmylene, pyrazolylene or thiazolylene For aspects C and D of the mvention, X is more preferably 1,3-phenylene, 2,6-pyπdιnylene, 1,3- pyrazolylene or 2,5-thιazolylene For aspect B of the mvention X is preferably pyrazolylene or thiazolylene For aspect B of the mvention X is more preferably 1,3-pyrazolylene or 2,5-thιazolylene For aspects B and D of the mvention R is preferably H, methoxy, 0(CH 2 ) 2 OH, 0(CH 2 ) 2 OCH 3 , 0(CH 2 ) 2 N(CH 3 ) 2 , 0(CH 2 ) 2 NHCH 3 , 0(CH 2 ) 2 NH 2 , CH 2 NHCH 3 , morpholinomethyl, 2-morpholιnoethoxy, 2R-2,3-dιhydroxy-l-propyloxy, 2S-2,3-dιhydroxy-l-propyloxy or l,3-dιhydroxy-2-propyloxy For aspects B and D of the mvention R is most preferably 0(CH 2 ) 2 OH or 0(CH 2 ) 2 NH 2 For aspect C of the invention R is preferably 0(CH 2 ) 2 N(CH 3 ) 2 , 0(CH 2 ) 2 NHCH 3 , 0(CH 2 ) 2 NH 2 , CH 2 NHCH 3 , morpholmomethyl, 2-mo holmoethoxy, 2R-2,3-dιhydroxy-l-propyloxy, 2S-2.3- dιhydroxy-1-propyloxy or l,3-dιhydroxy-2-propyloxy For aspect C of the invention R is most preferably 0(CH 2 ) 2 NH 2 For aspects B and C of the invention preferably R' and R 2 are each independently C,^ alkyl optionally substituted by OH, or R 1 and R 2 are taken together, with the C atom to which they are attached, to form a 3- to 7-membered nng optionally incorporating a hetero- moiety selected from O, S, SO, S0 2 and NR 6 , and which 3- to 7- membered ring is optionally substituted by one or more OH For aspects B and C of the invention more preferably R 1 and R 2 are each CH 3 , or R 1 and R 2 are taken together, with the C atom to which they are attached, to form a tetrahydropyran-4- yhdene, pιpeπdm-4-ylιdene, l-methylpιpendm-4-ylιdene, or 3,4-dιhydroxycyclopentylιdene moiety For aspects B and C of the mvention, yet more preferably R 1 and R 2 are taken together, with the C atom to which they are attached, to form a tetrahydropyran-4-ylιdene, cw-3,4-dιhydroxycyclopentylιdene, /ran.s-3,4-dιhydroxycyclopentylιdene or pιpeπdιn-4-yhdene moiety For aspects B and C of the mvention, most preferably R 1 and R 2 are taken together, with the C atom to which they are attached, to form a tetrahydropyran-4-ylιdene, pιpeπdιn-4-yhdene, or cιs-3,4- dihydroxycyclopentyhdene where the hydroxy substituents have a cu-relationship to the hydroxamate moiety For aspect D of the mvention, R 1 and R 2 are preferably taken together, with the C atom to which they are attached, to form a 3,4-dιhydroxycyclopentylιdene moiety For aspect D of the mvention, most preferably R 1 and R 2 are taken together, with the C atom to which they are attached, to form a cw-3,4-dιhydroxycyclopentylιdene group where the hydroxy substituents have a cw-relatιonshιp to the hydroxamate moiety For aspects A, B, C and D of the mvention R 3 is preferably methyl A preferred group of substances are those selected from the compounds of the Examples and the pharmaceutically acceptable salts and solvates thereof, especially the compounds of Examples 3, 6 and 14 below, and salts and solvates thereof The mvention further provides synthetic methods for the production of compounds, salts and solvates of the mvention, which are descπbed below and in the Examples The skilled man will appreciate that the compounds and salts of the invention could be made by methods other than those herem descπbed, by adaptation of the methods herem described and/or adaptation of methods known m the art, for example the art descπbed herein Specific art which may be mentioned includes WO 99/29667, "Comprehensive Orgamc Transformations" by RC Larock, VCH Publishers Inc (1989), "Advanced Organic Chemistry" by J March, Wiley Interscience (1985), "Desigmng Organic Synthesis" by S Warren, Wiley Interscience (1978), "Organic Synthesis - The Disconnection Approach" by S Warren, Wiley Interscience (1982), "Guidebook to Organic Synthesis" by RK Mackie and DM Smith, Longman (1982), "Protective Groups in Organic Synthesis" by TW Greene and PGM Wuts, John Wiley and Sons Inc (1999), and PJ Kocienski, m "Protecting Groups", Georg Thieme Verlag (1994), references therein, and any updated versions of the aforementioned standard works Where desired or necessary, the compound of formula (I) can be converted into a pharmaceutically acceptable salt thereof, conveniently by mixing together solutions of a compound of formula (I) and the desired acid or base, as appropπate The salt may be precipitated from solution and collected by filtration, or may be collected by other means such as by evaporation of the solvent In some cases, the salt may be the direct product of a reaction to make a compound or salt of the invention m a solvent, in which case no further transformation step would be necessary It is to be understood that the synthetic transformation methods mentioned herem may be earned out m vaπous different sequences m order that the desired compounds can be efficiently assembled The skilled chemist will exercise his judgement and skill as to the most efficient sequence of reactions for synthesis of a given target compound It will be apparent to those skilled m the art that sensitive functional groups may need to be protected and deprotected duπng synthesis of a compound of the mvention This may be achieved by conventional methods, for example as descπbed m "Protective Groups m Orgamc Synthesis" by TW Greene and PGM Wuts, John Wiley & Sons Inc (1999) The following methods are illustrative of the general synthetic procedures which may be adopted m order to obtain the compounds of the mvention In the synthetic methods below, unless otherwise specified, the substituents are as defined above with reference to the compounds of formula (I) as defined above with respect to aspects A, B, C and D A compound of formula (I) may be prepared directly from a corresponding acid or acid deπvative of formula (II) (") where Z is chloro, bromo, lodo, C, 3 alkyloxy or HO When prepared directly from the ester of formula (II), where Z is C, 3 alkyloxy, the reaction may be earned out by treatment of the ester with hydroxylamme, preferably up to a 3-fold excess of hydroxylamme, m a suitable solvent at from about room temperature to about 85°C The hydroxylamme is conveniently generated in situ from a suitable salt such as its hydrochloπde salt by conducting the reaction m the presence of a suitable base such as an alkali metal carbonate or bicarbonate, e g potassium carbonate Preferably the solvent is a mixture of methanol and tetrahydrofuran and the reaction is temperature is from about 65 to 70°C Alternatively, the ester (II, where Z is C, 3 alkyloxy) may be converted by conventional hydrolysis to the corresponding carboxylic acid (II, Z is HO) which is then transformed to the required hydroxamic acid of formula (I) [If the R, R 1 or R 2 moieties contain any free hydroxyl groups, these should be protected with groups inert to this functional group interconversion reaction sequence, and released following it, usmg standard methodology ] Preferably the hydrolysis of the ester is effected under basic conditions usmg about 2- to 6-fold excess of an alkali metal hydroxide in aqueous solution, optionally in the presence of a co-solvent, at from about room temperature to about 85°C Typically the co-solvent is a mixture of methanol and tetrahydrofuran or a mixture of methanol and 1,4-dioxan and the reaction temperature is from about 40 to about 70°C. The subsequent coupling step may be achieved using conventional amide-bond forming techniques, e.g via the acyl halide deπvative (II, Z is Cl, I or Br) and hydroxylamme hydrochlonde m the presence of an excess of a tertiary amine such as tπethylamine or pyndine to act as acid-scavenger, optionally m the presence of a catalyst such as 4-dιmethylamιnopyndιne, m a suitable solvent such as dichloromethane, at from about 0°C to about room temperature For convenience, pyridine may also be used as the solvent Such acyl halide substrates are available from the corresponding acid via conventional methods In particular, any one of a host of amino acid coupling variations may be used For example, the acid of formula (II) wherem Z is HO may be activated using a carbodiimide such as 1,3- dicyclohexylcarbodnmide or l-(3-dιmethylamιnopropyl)-3-ethylcarbodnmιde hydrochloπde (often referred to as "water-soluble carbodiimide" or "WSCDI") optionally m the presence of 1- hydroxybenzotπazole or l-hydroxy-7-aza-lH-l ,2,3-benzotπazole (HOAt) and/or a catalyst such as 4- dimethylaπunopyndine, or by using HOAt or a halotπsaminophosphonium salt such as bromotπs(pyττolιdιno)-phosphonιum hexafluorophosphate Either type of coupling is conducted m a suitable solvent such as dichloromethane, N-methylpyrrohdme (NMP)or dimethylformamide (DMF), optionally in the presence of pyridine or a tertiary amine such as N-methylmorpholine or N- ethyldiisopropylamine (for example when either the hydroxylamme or the activating reagent is presented in the form of an acid addition salt), at from about 0°C to about room temperature Typically, from 1 1 to 2 0 molecular equivalents of the activatmg reagent and from 1 0 to 4 0 molecular equivalents of any tertiary amme present are employed Preferred reagents for mediating the coupling reaction are HOAt, WSCDI and 0-(7-azabenzotπazol-l- yl)-l,l,3,3-tetramethyluronιum hexafluorophosphate (HATU) Preferably a solution of the acid (II, Z is HO) and N-ethyldnsopropylamine in a suitable solvent such as anhydrous dimethylformamide or anhydrous l-methylpyrrolιdιn-2-one, under nitrogen, is treated with up to a 1 5-fold excess of HATU at about room temperature followed, after about 15 to 30 mmutes, with up to about a 3-fold excess of hydroxylamme hydrochloπde and up to about a 4-fold excess of N- ethyldnsopropylamme, optionally m the same solvent, at the same temperature More preferably the acid (II, Z is HO) is reacted with a carbodiimide, HOBt and hydroxylamme hydrochlonde m pyndine m a suitable co-solvent such as dichloromethane An ester of formula (II, Z is C, 3 alkyloxy) may be prepared from an appropπate amme of formula (III) by sulphonylation with an appropπate compound of formula (TV), wherem R 10 is C, 3 alkyloxy and Z 1 is a leaving group such as Br, I or Cl (III) (iv) Preferably, Z 1 is chloro The reaction may be effected m the presence of an appropπate base in a suitable solvent at from about 0°C to about room temperature For example, when both R 1 and R 2 are hydrogen, an appropπate base is l,8-dιazabιcyclo[5 4 0]undec-7-ene and a suitable solvent is dichloromethane Alternatively, the base can be sodium lmidazohde An alternative method is to make a N-tπalkylsilyl dervative of (III), and mix with (IV) at room temperature in tetrahydrofuran (THF) in the presence of a catalytic amount of benzenesulphonic acid (BSA) Certain esters of formula (II, Z is C, 3 alkyloxy) wherem at least one of R 1 and R 2 is other than hydrogen may be conveniently obtamed from the α-carbanion of an ester of formula (II) wherem at least one of R' and R 2 is hydrogen by conventional C-alkylation procedures usmg an alkylatmg agent of formula (VA) or (VB) R'Z 1 or R 2 Z' (VA) Z 2 (CH 2 ) q Z 3 (VB), where the (CH 2 ) q moiety of (VB) optionally mcorporates a hetero- moiety selected from O, S, SO, S0 2 and NR 6 , and is optionally substituted by one or more optionally protected OH, and which NR 6 group may be optionally protected, wherem R 1 and R 2 are not hydrogen, Z 2 and Z 3 may be the same or different and are suitable leaving groups such as chloro, bromo, lodo, C,-C 4 alkanesulphonyloxy, tnfluoromethanesulphonyloxy or arylsulphonyloxy (e g benzenesulphonyloxy or p- toluenesulphonyloxy), and q is 3, 4, 5, 6 or 7 Other conditions are outlmed below - sections vn) and x) Preferably, Z 2 and Z 3 are selected from bromo, lodo and p-toluenesulphonyloxy The carbamon may be generated usmg an appropπate base in a suitable solvent, optionally m the presence of a phase transfer catalyst (PTC) Typical base-solvent combmations may be selected from lithium, sodium or potassium hydπde, lithium, sodium or potassium bιs(tπmethylsιlyl)aπude, lithium dnsopropylamide and butylhthium, potassium carbonate, sodium or potassium t-butoxide, together with toluene, ether, DMSO, 1,2-dιmethoxyethane, tetrahydrofuran, 1,4-dioxan, dimethylformamide, N,N- dimethylacetamide, l-methylpyrrohdin-2-one and any mixture thereof Preferably the base is sodium hydπde and the solvent is dimethylformamide, optionally with tetrahydrofuran as co-solvent, or l-methylpyrτohdιn-2-one For monoalkylation up to about a 10% excess of base is employed whilst, for dialkylation, from about 2 to about 3 molar equivalents are generally appropπate Typically, the carbamon is generated at about room temperature, under nitrogen, and subsequently treated with the required alkylatmg agent at the same temperature Clearly, when dialkylation is required and R 1 and R 2 are different, the substituents may be introduced m tandem m a "one-pot reaction" or in separate steps An amine of formula (III) may be obtained by standard chemical procedures Other amines of formula (III), when neither commercially available nor subsequently descnbed, can be obtained either by analogy with the processes described m the Preparations section below or by conventional synthetic procedures, in accordance with standard textbooks on organic chemistry or literature precedent, from readily accessible starting materials using appropriate reagents and reaction conditions Another way of making compounds of formula (II) where ZCO is an ester moiety, is via the reaction sequence (V) (VI) The appropnate sulphonyl chloπde (V) is reacted with compound (III - see above) optionally in the presence of a base and m a suitable solvent The resulting sulphonamide (VI) is reacted with a suitable base such as n-butyllithium, sodium hydπde or potassium t-butoxide in a suitable anhydrous non-protic solvent to generate the carbamon α to the sulphonamide moiety, which is then reacted with for example dimethyl carbonate or methyl chloroformate, in suitable conditions, either of which reagent would give the compound (II) where Z is methoxy Compounds of formula (I) where R contains a free NH, NH 2 and/or OH group (apart from on the hydroxarmc acid moiety) may conveniently be prepared from a corresponding N- or O-protected species (VII below) As such, compounds of formula (VII) where R p is a O- and/or N-protected version of a corresponding compound of the formula (I), are included m the scope of this mvention, with regard to aspects A, B, C and D of the mvention and the specific compounds of formula (I) mentioned herem, such as those mentioned m the Preparations, as appropπate, below Suitable protection / deprotection regimes are well known in the art, such as those mentioned m "Protective Groups m Organic Synthesis" by TW Greene and PGM Wuts, John Wiley & Sons Inc (1999) Suitable OH-protecting groups and regimes mclude the ethers such as t-butyloxy, tn(C )sιlyloxy, etc , and esters such as carbonates, sulphonates, C acylates, etc mentioned by Greene and Wuts, ibid chapter 2 Suitable NH-protecting groups and regimes can be found m Greene and Wuts, ibid chapter 7, and mclude amides such as "Boc", amines such as benzyl, etc Compounds of formula (VII) may be made by methods descπbed herem and /or by vaπation of methods descnbed herem which the skilled man will appreciate are routme vanations (VII) An example of a suitable OH-protecting group is the trimethylsilyl (TMS) group and the protection, reaction, deprotection sequence can be summansed by steps a) to c) below a) ClSιMe 3 (1 1 equiv per OH), WSCDI (1 1 to 1 2 equiv), HOBT or HOAT (1 to 1 1 equiv), b) NH 2 OH HCl (3 equiv) m DMF/pyndme or CH 2 Cl 2 /pyndιne (3/1 to 1/1) at rt for between 4 and 20 hours c) TMS group removed by acid work-up Another example of a suitable OH-protecting group is the t-butyl (*Bu) group which can be earned through the synthetic process and removed m the last step of the process An example of the route is outlmed m the scheme below (in relation to the synthesis of the compound of Example 3 - via compounds of the Preparations mentioned below) An example of a suitable NH-protecting group is the t-butoxycarbonyl (Boc) group This group can be introduced in standard ways, such as those delineated in the Examples and Preparations section below After the hydroxamic acid unit has been introduced, the Boc group can be removed for example by treatment of the N-Boc compound in methanol or dichloromethane saturated with HCl gas, at room temperature for 2 to 4 hours Compounds of formula (I) where R' and/or R 2 , either independently or together, contain a free NH, NH 2 and/or OH group (apart from on the hydroxamic acid moiety) may conveniently be prepared from a correspondmg N- and or O-protected species (XII below) As such, compounds of formula (XII) where R lp and/or R 2p is a O- and/or N-protected version of a correspondmg compound of the formula (I), are included in the scope of this invention, with regard to aspects A, B, C and D of the mvention and the specific compounds of formula (I) mentioned herein, such as those compounds of formula (XII) mentioned m the Preparations, as appropπate, below Suitable protection / deprotection regimes are well known in the art, such as those mentioned m "Protective Groups m Organic Synthesis" by TW Greene and PGM Wuts, John Wiley & Sons Inc ( 1999) Suitable OH-protecting groups and regunes mclude the ethers such as t-butyloxy, tn(C )sιlyloxy, etc , and esters such as carbonates, sulphonates, C acylates, etc mentioned by Greene and Wuts, ibid chapter 2 Suitable NH-protecting groups and regimes can be found in Greene and Wuts, ibid chapter 7, and mclude amides such as "Boc", amines such as benzyl, etc Compounds of formula (XII) may be made by methods descπbed herem and /or by vaπahon of methods descπbed herem which the skilled man will appreciate are routine vanations (XII) An example of a suitable OH-protecting group is the trimethylsilyl (TMS) group and the protection, reaction, deprotection sequence can be summarised by steps a) to c) below a) ClSιMe 3 (1 1 equiv per OH), WSCDI (1 1 to 1 2 equiv), HOBT or HOAT (1 to 1 1 equiv), b) NH 2 OH HCl (3 equiv) m DMF/pyndme or CH 2 Cl 2 /pyπdme (3/1 to 1/1) at rt for between 4 and 20 hours c) TMS group removed by acid work-up Another example of a suitable OH-protecting group is the t-butyl ('Bu) group which can be earned through the synthetic process and removed in the last step of the process An example of the route is outlined in the scheme below (in relation to the synthesis of the compound of Example 3 - via compounds of the Preparations mentioned below) An example of a suitable NH-protecting group is the t-butoxycarbonyl (Boc) group This group can be introduced in standard ways, such as those delineated m the Examples and Preparations section below After the hydroxamic acid unit has been introduced, the Boc group can be removed for example by treatment of the N-Boc compound in methanol or dichloromethane saturated with HCl gas, at room temperature for 2 to 4 hours An extension of the above is where the compound of formula (I) contams a free, OH, NH and/or NH 2 group in R 1 , R 2 and R (e g some Examples below) In thos case a suitable precursor could be the compound of formula (XIII) below (Xlll) where the substituents are as previously defined Compounds of formula (I) and appropπate mtermediates thereto where R 1 and R 2 are taken together as 3,4-dihydroxycyclopentyhdene can be made via the correspondmg lntermediacy of a correspondmg cyclopent-3-enylιdene moiety, viz . Cyclopentylidene mtermediates can be epoxidised to give the correspondmg epoxide using standard methods The epoxide can be reacted in a number of different methods to give the diol product By suitable choice of reagents, conditions etc , the skilled chemist can make diols with any desired stereochemistry, using well-known methods As such, compounds of the formula (VIII) and (IX) below are included in the scope of the invention, with regard to aspects A, B, C and D and also with respect to intermediates to appropriate individual compounds of formula (I) mentioned herein (VIII) (IX) Also mcluded m the mvention are mtermediates of formula (X) and (XI, where R p is defined as above for compounds of formula (VII) wherem P and P 1 represent standard OH and 1,2-dιol protecting groups mentioned m Greene and Wuts, ibid , chapter 2 P and P' are preferably taken together and form an acetomde moiety (X) (XI) Certain specific compounds of formulae (VIII), (IX), (X) and (XI) are mentioned m the Preparations below. Moreover, persons skilled m the art will be aware of vaπations of, and alternatives to, those processes described herein, including in the Examples and Preparations sections, which allow the compounds defined by formula (I) to be obtained, such as carrying out certain bond-forming or functional group interconversion reactions in different sequences Examples of the preparation of a number of intermediates and final compounds are outlined in the following synthetic schemes, where the abbreviations used are standard and well-known to the person skilled in the art. Routine variation of these routes can give all the required compounds of the invention. Route 1 (Pyridyl alcohols) i = NaH (1.1 equiv), HOCH 2 CHRl 1'ORIO (1 equiv) in toluene, reflux for 2 to 5 hours ii = n-BuLi (1.1 equiv), Bu 3 SnCl (1.1 equiv), THF, -70°C to room temperature. Or, Pd(PPh 3 )„ (0.01 to 0.05 equiv), [SnMe 3 ] 2 (1.1 equiv), dioxan, reflux for 2 to 5 hrs. iii = BSA (0.5 equiv), MeC0 2 CH 2 S0 2 Cl (1.2 equiv), THF, rt for 18 hours. iv = MeS0 2 Cl (1 2 equiv), Et 3 N (1 4 equiv), CH 2 C1 2 , rt, for an hour v = Et 3 SιH (3 equiv), CF 3 S0 3 H (0 1 equiv), TFA CH 2 C1 2 (1 1), rt, for 1-24 hrs vi = NaH (2 equiv), Me 2 C0 3 (4 equiv), toluene, reflux for 2 hours RlO-alcohol protecting group- e g benzyl or dioxalane (for diols) Rl 1 '-H or a protected alcohol vn = (VB), (1.3 equiv), K 2 C0 3 (3 equiv), DMSO, rt, 18-24 hours, or OtBu (2.5 equiv), (VA) or (VB) (excess), m THF, rt for 72 hours. vin = Stille couplιng-Pd(PPh 3 ) 4 (0.05 equiv), stannane (1.5 equiv), toluene, reflux for 4 to 20 hours. OR PdCI 2 (PPh 3 ) 2 (0.05 equiv), stannane (1.1 equiv), THF, reflux for 17 hours. ix = NH 4 + HC0 3 (excess) Pd(OH) 2 /C, AcOH, MeOH, reflux for 20 hours, OR 10% Pd/C, m MeOH or EtOH, 3.3 atmospheres, room temperature, for 6 to 17 hours,-both methods also deprotect any benzyl group. (2N HCl, dioxan (3.1), rt, 75 nuns at rt- deprotects the dioxalane) OR Pd(OH) 2 /C, NH 4 + HC0 3 (excess), m MeOH:dιoxan (2.5:1), 60°C for 2 hours. Rl 1 = H or deprotected alcohol Similarly when R1R2 when taken together, are a pipeπdine group: x = NaH (3 equiv), tetra-nBuNH 4 Br (1 equiv), BnN(CH 2 CH 2 Cl) 2 (0.95 equiv), NMP, 60°C for 6 hours. xi = When Rl 2 is Me, formaldehyde (4 equiv), Na(OAc) 3 BH (2 equiv), CH 2 C1 2 , 20 hrs at rt. When R12 is Boc, (Boc) 2 0 (1.05 equiv), Et 3 N (1.1 equiv), CH 2 C1 2 , rt for an hour. Route 2 (Phenyl alcohols) Or xii = nBuLi (1.1 equiv), B[OCH(CH 3 ) 2 ] 3 (1.5 equiv), THF, -70°C to rt. xui = Suzuki couplmg- arylboronic acid (1.2 to 1.5 equiv), CsF (2 to 2.6 equiv), P(o-tol) 3 (0.1 equiv), Pd 2 (dba) 2 (0.005 equiv), DME, reflux for 6 to 50 hours. xiv = Et 3 SiH (3 equiv), TFA:CH 2 C1 2 (1: 1), rt for 2 to 24 hours. xv = R S glycidol (1 equiv), Et 3 N (catalytic), MeOH, reflux for 20 hours. OR, Mitsunobu reaction -DEAD (1 5 equiv), PPh 3 (1 5 equiv), HOCH(Rl l')CH 2 OR13' (1.5 equiv) m THF, rt for 3 hours. Rl 1 ' is H or optionally protected alcohol and R13' is optionally protected alcohol For preparation 50 to 51 , requires Bn deprotection usmg the conditions descπbed in ix. Alternative route xxiv = i- NaH (2.2 equiv), Me 2 C0 3 (5 equiv), toluene, MeOH (catalytic), 90°C, overnight. ii- 0(CH 2 CH 2 Br) 2 (1.3 equiv), NMP, 90°C, 20 hrs. xxv = Grignard reagant (1.1 equiv), THF, -78°C to rt over approx hr. Rl 5 '-optionally protected alcohol, in prep 48 this is a t-butyl ether. R15-OH, for prep 48. Route 3 (Phenyl aminoalcohols) When R15 is a protecting group, eg. benzyl, deprotection, followed by protection usmg an alternative group eg Boc, can be used as shown below: xvi = IN HCl (1 to 2.3 equiv), acetone:dιoxan (1: 1), 70°C for 2 to 6 hours. xvii = Reductive amination-amine (5.5 equiv), Na(OAc) 3 BH (3 to 4 equiv), CH,C1 2 , rt, overnight. xviii = Pd(OH) 2 /C, MeOH, 50 psi, rt, 18 hrs. (Boc) 2 0 (1 to 1.1 equiv), Et 3 N (optional, 1 equiv), DMAP (optional, cat), CH 2 C1 2 , rt, 3 hrs. Route 4 (aminoalkyl phenyls) Route 5 (Heterocycles) 4 xx = iso-PrS0 2 Cl ( 1 equiv), Et 3 N (1.1 equiv), CH 2 C1 2 , 3hours at rt. xxi = n-BuLi (1.1 equiv), MeOCOCl (1.2 equiv), THF -78° to rt. xxii = 2,6-di-t-Bu-4-Me pyridine (2.5 equiv), (CF 3 S0 2 ) 2 0 (2.5 equiv), CH 2 C1 2 , 4°C to rt, 5 days. xxiii = Pd 2 (dba) 3 (0.02 equiv), vinyl triflate (1.1 equiv), Ph 3 As (0.21 equiv), Cul (0.1 equiv) in NMP, 75°C for 5 hrs. Thiazoles Route 6 (Cyclopentanediols) xxvi = NaH (1.1 equiv), tetra-nBuNH 4 Br ( 1 equiv), C1CH 2 CHCHCH 2 C1 (1.1 equiv), NMP, r.t for 3 hours, then NaH (1.1 equiv), 2 days. xxvii = NMO (1.1 equiv), Os0 4 (3 mol%), dioxan/water, r.t. 18 hours OR (a) AgOAc (2.3 equiv), AcOH, r.t for 18 hours (b) IN NaOH, dixoan/water xxviii = 2,2-Dιmethoxypropane (2 equiv), TsOH (0.1 equiv), DMF, 50°C for 4.5 hours. Biological Test Methods The biological activities of the compounds of the present invention were determined by the following test methods, which are based on the ability of the compounds to inhibit the cleavage of various fluorogenic peptides by MMPs 1, 2, 3, 9, 13 and 14. The assays for MMPs 2, 3, 9 and 14 are based upon the original protocol described in Fed.Euro.Biochem.Soc, 1992, 296. 263, with the minor modifications described below. Inhibition of MMP- 1 Enzyme Preparation Catalytic domain MMP-1 was prepared in Pfizer Central Research laboratories in a standard manner from sources known to the skilled person, including some of the references mentioned herein. A stock solution of MMP-1 (lμM) was activiated by the addition of aminophenyhnercuric acetate (APMA), at a final concentration of ImM, for 20 minutes at 37°C. MMP-1 was then diluted in Tris-HCl assay buffer (50mM Tris, 200mM NaCl, 5mM CaCl 2 , 20μM ZnS0 4 and 0.05% Brij 35, pH 7.5) to a concentration of lOnM. The final concentration of enzyme used in the assay was InM. Substrate The fluorogenic substrate used in this assay was Dnp-Pro-β-cyclohexyl-Ala-Gly-Cys(Me)-His-Ala-Lys- (N-Me-Ala)-NH 2 as originally described in Anal. Biochem., 1993, 212, 58. The final substrate concentration used in the assay was lOμM. Determination of Enzyme Inhibition The test compound was dissolved in dimethyl sulphoxide and diluted with assay buffer so that no more than 1% dimethyl sulphoxide was present. Test compound and enzyme were added to each well of a 96 well plate and allowed to equilibrate for 15 minutes at 37°C in an orbital shaker prior to the addition of substrate. Plates were then incubated for 1 hour at 37°C prior to determination of fluorescence (substrate cleavage) using a fluorimeter (Fluostar; BMG LabTechnologies, Aylesbury, UK) at an excitation wavelength of 355 nm and emission wavelength of 440 nm The potency of inhibition was measured from the amount of substrate cleavage obtained using a range of test compound concentrations and, from the resulting dose-response curve, an IC 50 value (the concentration of inhibitor required to inhibit 50% of the enzyme activity) was calculated Inhibition of MMP-2. MMP-3 and MMP-9 Enzyme Preparation Catalytic domains MMP-2, MMP-3 and MMP-9 were prepared m Pfizer Central Research laboratones in a standard manner from sources known to the skilled person, mcludmg some of the references mentioned herem A stock solution of MMP-2, MMP-3 or MMP-9 (lμM) was activated by the addition of APMA For MMP-2 and MMP-9, a final concentration of ImM APMA was added, followed by mcubation for 1 hour at 37°C MMP-3 was activated by the addition of 2mM APMA, followed by mcubation for 3 hours at 37°C The enzymes were then diluted m Tns-HCl assay buffer (lOOmM Tπs, lOOmM NaCl, lOmM CaCl 2 and 0 16% Bnj 35, pH 7.5) to a concentration of lOnM The final concentration of enzyme used m the assays was InM. Substrate The fluorogenic substrate used m this screen was Mca-Arg-Pro-Lys-Pro-Tyr-Ala-Nva-Trp-Met- Lys(Dnp)-NH 2 (Bachem Ltd., Essex, UK) as ongmally descnbed m J.Biol.Chem , 1994, 269, 20952. This substrate was selected because it has a balanced hydrolysis rate against MMPs 2, 3 and 9 (I -^/ -,,. of 54,000, 59,400 and 55,300 s "1 M ' respectively). The final substrate concentration used in the assay was 5μM. Determination of Enzyme Inhibition The test compound was dissolved m dimethyl sulphoxide and diluted with assay buffer so that no more than 1% dimethyl sulphoxide was present Test compound and enzyme were added to each well of a 96 well plate and allowed to equilibrate for 15 mmutes at 37°C m an orbital shaker pnor to the addition of substrate. Plates were then mcubated for 1 hour at 37°C, pnor to determination of fluorescence usmg a fluoπmeter (Fluostar; BMG LabTechnologies, Aylesbury, UK) at an excitation wavelength of 328nm and emission wavelength of 393nm The potency of inhibition was measured from the amount of substrate cleavage obtained usmg a range of test compound concentrations and, from the resulting dose-response curve, an IC 50 value (the concentration of inhibitor required to inhibit 50% of the enzyme activity) was calculated Inhibition of MMP- 13 Enzyme Preparation Human recombinant MMP-13 was prepared by PanVera Corporation (Madison, Wisconsin) and characterised at Pfizer Central Research laboratories A 1 9 mg/ml stock solution was activated with 2mM APMA for 2 hours at 37°C MMP-13 was then diluted m assay buffer (50mM Tns, 200mM NaCl, 5mM CaCl 2 , 20μM ZnCl 2 and 0 02% Bnj 35, pH 7 5) to a concentration of 5 3nM The final concentration of enzyme used in the assay was 1 3nM Substrate The fluorogenic substrate used m this screen was Dnp-Pro-Cha-Gly-Cys(Me)-Hιs-Ala-Lys(NMA)-NH 2 The final substrate concentration used in the assay was lOμM Determination of Enzyme Inhibition The test compound was dissolved m dimethyl sulphoxide and diluted with assay buffer so that no more than 1% dimethyl sulphoxide was present Test compound and enzyme were added to each well of a 96 well plate The addition of substrate to each well mitiated the reaction Fluorescence intensity was determined usmg a 96 well plate fluoπmeter (Cytofluor II, PerSeptive Biosystems, Inc , Framingham, MA) at an excitation wavelength of 360nm and emission wavelength of 460nm The potency of inhibition was measured from the amount of substrate cleavage obtamed usmg a range of test compound concentrations and, from the resultmg dose-response curve, an IC 50 value (the concentration of inhibitor required to inhibit 50% of the enzyme activity) was calculated Inhibition of MMP- 14 Enzyme Preparation Catalytic domam MMP- 14 was prepared m Pfizer Central Research laboratoπes m a standard manner from sources known to the skilled person, mcludmg some of the references mentioned herem A lOμM enzyme stock solution was activated for 20 mmutes at 25 °C followmg the addition of 5 μg/ml of trypsm (Sigma, Dorset, UK) The trypsm activity was then neutralised by the addition of 50μg/ml of soyabean trypsm inhibitor (Sigma, Dorset, UK), pnor to dilution of this enzyme stock solution m Tns-HCl assay buffer (lOOmM Tns, lOOnM NaCl, lOmM CaCl 2 , 0 16% Bnj 35, pH 7 5) to a concentration of lOnM The final concentration of enzyme used m the assay was InM Substrate The fluorogenic substrate used m this screen was Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH 2 (Bachem Ltd , Essex, UK) as described in J Biol Chem , 1996, 271, 17119 Determination of enzyme inhibition This was performed in the same manner as descnbed for MMPs 2, 3 and 9 For use m mammals, mcludmg humans, the compounds of formula (I) or their salts or solvates of such compounds or salts, can be administered alone, but will generally be administered in admixture with a pharmaceutically or veteπnanly acceptable diluent or earner selected with regard to the mtended route of administration and standard pharmaceutical practice For example, they can be administered orally, including subhngually, m the form of tablets containing such excipients as starch or lactose, or in capsules or ovules either alone or m admixture with excipients, or in the form of elixirs, solutions or suspensions containmg flavouring or colouring agents The compound or salt could be incorporated mto capsules or tablets for targetting the colon or duodenum via delayed dissolution of said capsules or tablets for a particular tune followmg oral administration Dissolution could be controlled by susceptibility of the formulation to bactena found m the duodenum or colon, so that no substantial dissolution takes places before reachmg the target area of the gastrointestinal tract The compounds or salts can be injected parenterally, for example, intravenously, intramuscularly or subcutaneously. For parenteral administration, they are best used m the form of a steπle aqueous solution or suspension which may contain other substances, for example, enough salt or glucose to make the solution isotonic with blood They can be administered topically, m the form of steπle creams, gels, suspensions, lotions, omtments, dusting powders, sprays, drug-incorporated dressings or via a skm patch For example they can be incorporated mto a cream consistmg of an aqueous or oily emulsion of polyethylene glycols or liquid paraffin, or they can be incorporated mto an ointment consistmg of a white wax soft paraffin base, or as hydrogel with cellulose or polyacrylate deπvatives or other viscosity modifiers, or as a dry powder or liquid spray or aerosol with butane/propane, HFA or CFC propellants, or as a drug-incorporated dressmg either as a tulle dressmg, with white soft paraffin or polyethylene glycols impregnated gauze dressings or with hydrogel, hydrocolloid, algmate or film dressings The compound or salt could also be admmistered mtraocularly as an eye drop with appropπate buffers, viscosity modifiers (e.g cellulose denvatives), preservatives (e.g benzalkonium chloπde (BZK)) and agents to adjust temcity (e.g sodium chlonde) Such formulation techniques are well-known in the art In some instances the formulations may advantageously also contain an antibiotic All such formulations may also contain appropnate stabilisers and preservatives For veterinary use, a compound of formula (I), or a veteπnanly acceptable salt thereof, or a veteπnaπly acceptable solvate of either entity, is admmistered as a suitably acceptable formulation m accordance with normal veterinary practice and the veterinary surgeon will determine the dosmg regimen and route of administration which will be most appropnate for a particular animal Reference to treatment mcludes prophylaxis as well as alleviation of established conditions, or the symptoms thereof For oral and parenteral administration to animal (inc. human) patients, the daily dosage level of the compounds of formula (I) or their salts will be from 0.001 to 20, preferably from 0.01 to 20, more preferably from 0.1 to 10, and most preferably from 0.5 to 5 mg kg (in single or divided doses). Thus tablets or capsules of the compounds will contain from 0.1 to 500, preferably from 50 to 200, mg of active compound for administration singly or two or more at a time as appropriate. For topical administration to animal (inc. human) patients with chronic wounds, the daily dosage level of the compounds, in suspension or other formulation, could be from 0.001 to 30mg/ml, preferably from 0.01 to 10 mg/ml. The physician or veterinary surgeon in any event will determine the actual dosage which will be most suitable for a an individual patient and it will vary with the age, weight and response of the particular patient. The above dosages are exemplary of the average case; there can of course be individual instances where higher or lower dosage ranges are merited, and such are within the scope of this invention. Thus the invention provides a pharmaceutical composition comprising a compound of formula (I), or a pharmaceutically acceptable salt thereof, or solvate thereof, together with a pharmaceutically acceptable diluent or carrier. The invention also provides a compound of formula (I), or a pharmaceutically acceptable salt thereof, or solvate thereof, or a pharmaceutical composition containing any of the foregoing, for use as a human medicament. In yet another aspect, the invention provides the use of a compound of formula (I), or a pharmaceutically acceptable salt thereof, in the manufacture of a human medicament for the treatment of a condition mediated by one or more MMPs. Moreover, the invention provides the use of a compound of formula (I), or a pharmaceutically acceptable salt thereof, for the manufacture of a human medicament for the treatment of atherosclerotic plaque rupture, myocardial infarction, heart failure, restenosis, stroke, periodontal disease, tissue ulceration, wounds, skin diseases, cancer metastasis, tumour angiogenesis, age-related macular degeneration, fibrotic disease, rheumatoid arthritis, osteoarthritis and inflammatory diseases dependent on migratory inflammatory cells. Additionally, the invention provides a method of treating a medical condition for which a MMP inhibitor is indicated, in an animal such as a mammal (including a human being), which comprises administering to said animal a therapeutically effective amount of a compound of formula (I), or a pharmaceutically acceptable salt thereof, or a pharmaceutically acceptable solvate of either entity, or a pharmaceutical composition containing any of the foregoing. Still further, the invention provides a method of treating atherosclerotic plaque rupture, myocardial infarction, heart failure, restenosis, stroke, periodontal disease, tissue ulceration, wounds, skm diseases, cancer metastasis, tumour angiogenesis, age-related macular degeneration, fibrotic disease, rheumatoid arthntis, osteoarthπtis and mflammatory diseases dependent on migratory inflammatory cells, m a animal (mcludmg a human being), which comprises admmistenng to said animal a therapeutically effective amount of a compound of formula (I), or a pharmaceutically or veteπnanly acceptable salt thereof, or a pharmaceutically acceptable solvate of either entity, or a pharmaceutical composition contammg any of the foregoing Biological data The compounds of Examples 3, 4, 5, 6, 7, 10 and 14 gave the following IC 50 values (m nM concentrations) in tests mentioned above MMP-3 MMP-2 MMP-1 MMP- 14 MMP-9 <10 >100 >1000 >2000 >70 The syntheses of the compounds of the mvention and of the mtermediates for use therein are illustrated by the followmg Examples and Preparations EXAMPLES AND PREPARATIONS Room temperature (rt) means 20 to 25°C Flash chromatography refers to column chromatography on silica gel (Kieselgel 60, 230-400 mesh) Melting points are uncorrected 'H Nuclear magnetic resonance (NMR) spectra were recorded usmg a Bruker AC300, a Vanan Unity Inova-300 or a Vaπan Umty Inova- 400 spectrometer and were m all cases consistent with the proposed structures Charactenstic chemical shifts are given m parts-per-milhon downfield from tetramethylsilane usmg conventional abbreviations for designation of major peaks e g s, smglet, d, doublet, t, tπplet, q, quartet, m, multiplet, br, broad Mass spectra were recorded usmg a Finnigan Mat TSQ 7000 or a Fisons Intruments Tno 1000 mass spectrometer LRMS means low resolution mass spectrum and the calculated and observed ions quoted refer to the isotopic composition of lowest mass Hexane refers to a mixture of hexanes (hplc grade) b p 65-70°C Ether refers to diethyl ether Acetic acid refers to glacial acetic acid l-Hydroxy-7-aza-lH- 1 ,2,3-benzotπazole (HOAt), N-[(dιmethylamιno)- 1 H- 1 ,2,3-tnazolo[4,5-b]pyπdm- 1 -ylmethylene]-N- methylmethaninium hexafluorophosphate N-oxide (HATU) and 7-azabenzotπazol-l- yloxytns(pyrrolidino)phosphonium hexafluorophosphate (PyAOP) were purchased from PerSeptive Biosystems U K Ltd "Me" is methyl, "Bu" is butyl, "Bn" is benzyl Other abbreviations and terms are used in conjunction with standard chemical practice Example 1 N-Hydroxy 2-[(4-{4-[6-(2-hydroxyethoxy)pyπdιn-2-yl]-3-methylphenyl}pιpeπdιn-l-yl)sulphonyl]-2- methylpropanamide N,N-Dιmethylformamιde (10ml) was added to a solution of the acid from preparation 70 (430mg, 0.93mmol) m pyridine (5ml), followed by chlorotπmethylsilane (130μl, 1.03mmol) and the solution stured for 1 'A hours l-(3-Dιmethylamιnopropyl)-3-ethylcarbodιιmιde hydrochloπde (215mg, 1.1 lmmol) and 1 -hydroxybenzotriazole hydrate (130mg, 0 93mmol) were added, and the reaction stured for a further 2 hours. Hydroxylamme hydrochloπde (195mg, 2 8mmol) was then added, and the reaction stured at room temperature overnight. The reaction mixture was acidified to pH 1 usmg 2N hydrochloπc acid, stirred for an hour, and then the pH re-adjusted to pH 4 Water (50ml) was added, the resultmg precipitate filtered, washed with water and dπed under vacuum This solid was puπfied by column chromatography on silica gel usmg dιchloromethane:methanol:0.88 ammonia (90.10T) as eluant to afford the title compound as a white solid, (220mg, 49%). mp 137-140°C Η nmr (DMSO-d^, 300MHz) δ: 1.50 (s, 6H), 1.61 (m, 2H), 1.80 (m, 2H), 2.36 (s, 3H), 2.68 (m, IH), 3.05 (m, 2H), 3.72 (m, 4H), 4.25 (t, 2H), 4.79 (t, IH), 6.76 (d, IH), 7.05 (d, IH), 7.17 (m, 2H), 7.35 (d, IH), 7.76 (dd, IH), 9.00 (s, IH), 10.55 (s, IH). Example 2 N-Hydroxy 2- {f4-(4-{6-f2-(methoxy)ethoxy]pyridm-2-yl}-3-methylphenyl)pipendm-l-yl]sulphonyl}-2- methylpropanamide 0-(7-Azabenzotnazol-l-yl)-N,N,N'N'-tetramethyluronιum hexafluorophosphate (425mg, 0 95mmol) and N-ethyldnsopropylamine (150μl, 0 70mmol) were added to a solution of the acid from preparation 71 (300mg, 0.63mmol) in N,N-dimethylformamide (10ml), and the solution stirred at room temperature for 30 minutes. Hydroxylamine hydrochloride (158mg, 1.9mmol) and additional N-ethyldiisopropylamine (410μl, 1.9mmol) were added, and the reacton stiπed at room temperature overnight. The reaction mixture was diluted with water (20ml), and pH 7 buffer solution (20ml), and then extracted with ethyl acetate (3x30ml). The combined organic extracts were washed with brine (3x), water (2x), then dried (MgS0 4 ), filtered and evaporated in vacuo. The residue was triturated with di-isopropyl ether to afford the title compound as an off-white solid, (220mg, 71%). mp 134-138°C Η nmr (DMSO-d^, 300MHz) δ: 1.48 (s, 6H), 1.61 (m 2H), 1.80 (m, 2H), 2.36 (s, 3H), 2.66 (m, IH), 3.05 (m, 2H), 3.28 (s, 3H), 3.62 (t, 2H), 3.78 (m, 2H), 4.38 (t, 2H), 6.78 (d, IH), 7.06 (d, IH), 7.16 (m, 2H), 7.35 (d, IH), 7.76 (m, IH). Anal. Found: C, 59.65; H, 7.12; N, 7.69. , 4 H 33 N 3 O 6 S;0.2i-Pr 2 O requires C, 59.59; H, 7.04; N, 8.04%. Example 3 N-Hydroxy 4- {[4-(4-{6-[2-hydroxyethoxy]pyridin-2-yl}-3-methylphenyl)piperidin-l- yl]sulphonyl}tetrahydro-2H-pyran-4-carboxamide Chlorotrimethylsilane (2.1ml, 16.46mmol) was added to a solution of the acid from preparation 72 (7.55g, 14.96mmol) in N,N-dimethylformamide (150ml), and pyridine (150ml), and the solution stirred at room temperature under a nitrogen atmosphere for 1 hour. l-(3-Dimethylaminopropyl)-3- ethylcarbodiimide hydrochloride (3.44g, 17.95mmol) and l-hydroxy-7-azabenzotriazole (2.04g, 14.96mmol) were added, and stirring was continued for a further 45 minutes. Hydroxylamine hydrochloride (3.12g, 44.8mmol) was then added and the reaction stirred at room temperature forJ2 hours. The reaction mixture was acidified to pH 2 using hydrochloric acid, stirred for 30 minutes, and the pH then re-adjusted to pH 4 using IN sodium hydroxide solution. The mixture was extracted with ethyl acetate (3x), the combined organic extracts washed with brine, dried (MgS0 4 ), filtered and evaporated in vacuo. The residue was purified by column chromatography on silica gel using ethyl acetate as eluant, and recrystallised from methanol ethyl acetate to afford the title compound as a white solid, (3.75g, 48%). mp 193-194°C 'H nmr (DMSO-dβ, 400MHz) δ: 1.61 (m, 2H), 1.79 (m, 2H), 1.92 (m, 2H), 2.36 (m 5H), 2.62 (m, IH), 3.01 (m, 2H), 3.19 (m, 2H), 3.70 (m, 4H), 3.82 (m, 2H), 4.25 (t, 2H), 4.75 (br, t, IH), 6.70 (d, IH), 7.01 (d, IH), 7.12 (m, 2H), 7.30 (d, IH), 7.62 (dd, IH), 9.10 (s, IH), 10.94 (s, IH). LRMS : m/z 520 (M+l) + Anal. Found: C, 57.73; H, 6.39; N, 7.99. C 25 H 33 N 3 0 7 S requires C, 57.79; H, 6.40; N, 8.09%. Alternative route: Hydrogen chloride gas was bubbled through a solution of the tert-butyl ether from preparation 133 (3.0g, 5.22mmol) in anhydrous trifluoroacetic acid (30ml) and dichloromethane (30ml) for 10 minutes, then stirred at room temperature overnight. Nitrogen gas was bubbled through the reaction mixture for lhour and then 5N NaOH solution until the solution was pH6. The resulting precipitate was cooled to 0°C, filtered and washed with cold water. The resulting solid was dissolved in hot ethyl acetate (500ml) and the organic layer was washed with water (3x250ml) and brine (250ml) and then dried (Na 2 S0 4 ), filtered and concentrated in vacuo. On cooling to 0°C overnight a solid formed and was filtered, washed with cold ethyl acetate and dried. The title compound was obtained as a beige solid (1.6g, 60%). Example 4 N-Hydroxy 4- { [4-(4- { 6- [(2S)-2,3 -dihydroxy- 1 -propoxy]pyridin-2-yl} -3 -methylphenyl)piperidin- 1 - yl] sulphonyl } tetrahydro-2H-pyran-4-carboxamide Chlorotrimethylsilane (168μl, 1.32mmol) was added to a solution of the acid from preparation 73 (318mg, 0.60mmol) in dichloromethane (6ml), and pyridine (2ml), and the solution stirred at room temperature under a nitrogen atmosphere for 1 hour. l-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (138mg, 0.72mmol) and l-hydroxy-7-azabenzotriazole (90mg, 0.66mmol) were added, and stirring was continued for a further hour. Hydroxylamine hydrochloride (124mg, 1.80mmol) was added and the reaction stirred at room temperature for 2 hours. The reaction mixture was evaporated in vacuo, the residue dissolved in methanol, the solution acidified to pH 1 using hydrochloric acid (2M), then stirred for 10 minutes The solution was diluted with water, the pH adjusted to 6, and the resulting precipitate filtered and dried The solid was purified by column chromatography on silica gel using dichloromethane methanol (90 10) as eluant, and recrystallised from methanol/di-isopropyl ether to give the title compound as a white solid, (200mg, 60%) Η n r (DMSO-d 6 , 400MHz) δ 1 61 (m, 2H), 1 79 (m, 2H), 1 92 (m, 2H), 2 36 (m, 5H), 2 63 (m, IH), 3 03 (m, 2H), 3 08-3 31 (m, 3H), 3 40 (m, 2H), 3 68-3 89 (m, 4H), 4 15 (m, IH), 4 25 (m, IH), 4 56 (br, s, IH), 4 80 (br, s, IH), 6 75 (d, IH), 7 04 (d, IH), 7 14 (m, 2H), 7 34 (d, IH), 7 75 (m, IH), 9 14 (s, IH), 10 96 (s, IH) LRMS m/z 550 (M+l) + Anal Found C, 50 70, H, 6 00, N, 6 93 , 6 H 35 N 3 O 8 S,0 6H 2 0 requires C, 50 97, H, 6 21, N, 6 86% Example 5 N-Hydroxy 4-{[4-(4-{6-[(2R)-2,3-dιhydroxy-l-propoxy]pyndm-2-yl}-3-methylphenyl)pιpeπdm-l- yl]sulρhonyl}tetrahydro-2H-pyran-4-carboxamιde The title compound was prepared from the acid from preparation 74, followmg the procedure descnbed in example 4 The crude product was puπfied by crystallisation from ethyl acetate to give an off-white solid (180mg, 58%) mp 125-130°C 'H nmr (DMSO , 400MHz) δ 1 60 (m, 2H), 1 78 (m, 2H), 1 90 (m, 2H), 2 36 (m, 5H), 2 64 (m, IH), 3 02 (m, 2H), 3 20 (m, 2H), 3 40 (m, 2H), 3 72 (m, 2H), 3 78 (m, IH), 3 83 (m, 2H), 4 14 (m, 1H , 4 24 (m, IH), 4 55 (dd, IH), 4 80 (d, IH), 6 75 (d, IH), 7 03 (d, IH), 7 15 (m, 2H), 7 32 (d IH), 7 75 (m, IH), 9 14 (s, IH), 10 95 (s, IH) LRMS m/z 572 (M+23) + Anal Found C, 55 32, H, 6 57, N, 7 28 C 26 H 35 N 3 O g S,H 2 0 requues C, 55 02, H, 6 57, N, 7 40% Example 6 N-Hydroxy 4- {[4-(4- {6-[2-hydroxyethoxy]pyπdιn-2-yl}-3-methylphenyl)pιpeπdιn-l-yl]sulphonyI}- pιpendιne-4-carboxamιde dihydrochloπde Hydrogen chlonde gas was bubbled through an ice-cold solution of the hydroxamic acid from preparation 87 (135mg, 0.22mmol) in methanol (20ml), and die solution was stured at room temperature The reaction mixture was evaporated m vacuo, and the residue azeotroped with methanol The solid was recrystallised from methanol/ether to afford the title compound as a white solid, (88mg, 64%). 'H nmr (DMSO-dβ, 400MHz) δ- 1.63 (m, 2H), 1.80 (m, 2H), 2.07 (m, 2H), 2.35 (s, 3H), 2.56-2.72 (m, 5H), 2.08 (m, 2H), 2.38 (m, 2H), 3 72 (m, 4H), 4.24 (t, 2H), 4 44-4 67 (br, s, 2H), 6.76 (d, IH), 7.04 (d, IH), 7.17 (m, 2H), 7.34 (d, IH), 7.75 (m, IH), 8.97 (m, IH), 9.18 (m, IH). LRMS : m/z 519 (M+l) + Example 7 N-Hydroxy 4- { [4-(4- {6-[2-hydroxyethoxy]pyridιn-2-yl} -3-methylphenyl)pιpeπdιn- 1 -yl]sulphonyl} - 1 - methyl-pιpendιne-4-carboxamιde The title compound was prepared from the acid from preparation 75 and hydroxylamme hydrochlonde followmg a similar procedure to that descnbed m example 1. The reaction mixture was acidified to pH 2 usmg hydrochloπc acid, this mixture stured for 45 minutes, then basified to pH 8 using sodium hydroxide solution (2N) This solution was extracted with ethyl acetate (3x), the combined organic extracts washed with water, then brine, dried (Na 2 S0 4 ), filtered and evaporated in vacuo. The residue was dried at 60°C, under vacuum to afford the title compound (39mg, 8%). Η nmr (DMSO-d 6 , 400MHz) δ: 1.60 (m, 2H), 1.78 (m, 4H), 1.86 (m, 2H), 2.8 (s, 3H), 2.35 (s, 3H), 2.40 (m, 2H), 2.59-2.75 (m, 3H), 3.01 (m, 2H), 3.68 (m, 4H), 4.25 (t, 2H), 4.75 (t, IH), 6.75 (d, IH), 7.03 (d, IH), 7.15 (m, 2H), 7.32 (d, IH), 7.74 (m, IH), 9.06 (br, s, IH), 10.88 (br, s, IH). LRMS : m/z 533 (M+l) + Anal. Found: C, 57.91 ; H, 6.82; N, 10.24. C 26 H 36 N 4 O 6 S;0.3H 2 O requires C, 58.04; H, 6.86; N, 10.41%. Example 8 N-Hydroxy 2-[4-(4- {3-[(2S)-2,3-dihydroxy- l-propoxy]phenyl} -3-methylphenyl)-piperidin- 1 - ylsulphonyl]-2-methylpropanamide The title compound was prepared from the acid from preparation 77, following a similar procedure to that described in example 3. The crude product was recrystallised from methanol/di-isopropyl ether, to give the desired product (75mg, 24%) as a white solid. The mother liquors were evaporated in vacuo, and purified by column chromatography on silica gel using an elution gradient of dichloromethane:methanol (98:2 to 95:5) to give an additional (38mg, 12%) of the desired product, mp 152-154°C Η nmr (DMSO-d , 400MHz) δ: 1.44 (s, 6H), 1.60 (m, 2H), 1.78 (m, 2H), 2.18 (s, 3H), 2.61 (m, IH), 3.02 (m, 2H), 3.39 (m, 2H), 3.71 (m, 3H), 3.82 (m, IH), 3.98 (m, IH), 4.56 (m, IH), 4.82 (m, IH), 6.82 (m, 3H), 7.08 (m, 2H), 7.12 (s, IH), 7.26 (m, IH), 8.94 (s, IH), 10.69 (s, IH). LRMS : m/z 529 (M+23) + Anal. Found: C, 58.10; H, 6.70; N, 5.09. C 25 H 34 N 2 O 7 S;0.5MeOH requires C, 58.60; H, 6.94; N, 5.36%. Example 9 N-Hydroxy 4- {4-[4-(3-[(2R)-2,3-dihydroxy-l-propoxy]phenyl)-3-methylphenyl]-piperidin-l- y lsulphonyl } -tetrahydro-(2H)-pyran-4-carboxamide H Chlorotrimethylsilane (45μl, 0.37mmol) was added to a solution of the acid from preparation 79 (90mg, 0.17mmol) in dichloromethane (2ml), and pyridine (1ml), and the solution stirred at room temperature under a nitrogen atmosphere for 1 hour. l-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (40mg, 0.21mmol) and l-hydroxy-7-azabenzotriazole (26mg, 0.19mmol) were added, and stirring was continued for a further hour. Hydroxylamine hydrochloride (36mg, 0.51mmol) was then added and the reaction stirred at room temperature for a further 2 hours. The reaction mixture was diluted with methanol (5ml), acidified to pH 1 using hydrochloric acid, and the mixture stiπed vigorously for an hour. The mixture was extracted with dichloromethane (3x30ml), the combined organic extracts dried (Na 2 S0 4 ), filtered and evaporated. The residue was purified by column chromatography on silica gel using dichloromethane:methanol (90:10) as eluant to afford the title compound as an off-white solid, (40mg, 43%). mp 141-145°C Η nmr (DMSOA, 400MHz) δ: 1.60 (m, 2H), 1.78 (m, 2H), 1.90 (m, 2H), 2.20 (s, 3H), 2.38 (m, 2H), 2.62 (m, IH), 3.03 (m, 2H), 3.20 (m, 2H), 3.42 (m, 2H), 3.66-3.90 (m, 6H), 4.01 (m, IH), 4.60 (m, IH), 4.90 (m, IH), 6.84 (m, 3H), 7.14 (m, 3H), 7.30 (m, IH), 9.18 (s, IH), 10.98 (IH, s). LRMS : m z 571 (M+23) + Anal. Found: C, 59.22; H, 6.80; N, 5.11. Cj 7 H 36 N 2 0,S requires C, 59.11; H, 6.61; N, 5.11%. Example 10 N-Hydroxy 4- {4-[4-(3-{(2S)-2-hydroxy-2-hydroxymethyl}ethoxyphenyl)-3-methylphenyl]-piperidin-l- ylsulphonyl}-tetrahydro-2H-pyran-4-carboxamide The title compound was prepared, from the acid from preparation 80, following a similar procedure to that described in example 9 The crude product was triturated with methanol/di-isopropyl ether, and the resulting precipitate filtered and dπed to afford the title compound as a buff-coloured solid, (158mg, 45%) mp 132-134°C Η nmr (DMSO-d,, 400MHz) δ 1 60 (m, 2H), 1 78 (m, 2H), 1 90 (m, 2H), 2 20 (s, 3H), 2 38 (m, 2H), 2 62 (m, IH), 3 02 (m, 2H), 3 20 (m, 2H), 3 42 (dd, 2H), 3 68-3 90 (m, 6H), 4 00 (m, IH), 4 60 (t, IH), 4 97 (d, IH), 6 81 (m, 2H), 6 90 (m, IH), 7 08 (s, 2H), 7 15 (s, IH), 7.29 (dd, IH), 9 14 (s, IH), 10 98 (s, IH) Example 11 N-Hydroxy 4- {4-[4-(3- { 1 ,3-dιhydroxy-2-propoxyphenyl)-3-methylphenyl]-pιpeπdm- 1 -ylsulphonyl} - tetrahydro-2H-pyran-4-carboxamιde The title compound was obtamed (25%) as a white solid, from the acid from preparation 78 and hydroxylamme hydrochlonde, usmg a similar procedure to that descnbed m example 9. 'H nmr (DMSO-d«, 400MHz) δ- 1.60 (m, 2H), 1.79 (m, 2H), 1.90 (m, 2H), 2.20 (s, 3H), 2.39 (m, 2H), 2.62 (m, IH), 3.02 (m, 2H), 3.20 (m, 2H), 3.57 (m, 4H), 3.70 (m, 2H), 3.84 (m, 2H), 4 24 (m, IH), 4 78 (m, 2H), 6.82 (d, IH), 6.90 (m, 2H), 7.14 (m, 3H), 7.28 (m, IH), 9 18 (br, s, IH). LRMS : m/z 570 (M+23) + Anal Found- C, 56.98; H, 6.65, N, 5 15. C 27 H 36 N 2 0 8 S;H 2 0 requires C, 57.22; H, 6 76, N, 4.94%. Example 12 N-Hydroxy 2-{[4-(4-{3-[2-(methylammo)ethoxy]phenyl}-3-methylphenyl)-pιpendm-l-yl]sulphonyl}-2- methylpropanamide hydrochloride Dichloromethane saturated with hydrogen chloride (12ml) was added to a solution of the hydroxamic acid from preparation 88 (120mg, 0.2mmol) in dichloromethane (1ml), and the reaction stined at room temperature for 4 hours. The resultmg precipitate was filtered, then washed with, dichloromethane, ether, then dned under vacuum at 60°C, to afford the title compound as a solid, (90mg, 85%). mp 180-184°C 'H nmr (DMSO A, 400MHz) δ: 1.44 (s, 6H), 1.60 (m, 2H), 1.78 (m, 2H), 2.18 (s, 3H), 2.59 (m, 3H), 3.02 (m, 2H), 3.28 (m, 2H), 3J2 (m, 2H), 4.23 (t, 2H), 6.90 (m, 3H), 7.08 (s, 2H), 7.16 (s, IH), 7.34 (m, IH), 8.83 (br s, 2H), 10.80 (s, IH). LRMS : m z 490 (M+l) + Anal. Found: C, 54.25; H, 6.93; N, 7.44. C 25 H 35 N 3 O 5 S;HCl;H 2 O;0.1CH 2 Cl 2 requires C, 54.56; H, 6.97; N, 7.60%. Example 13 N-Hydroxy 2-[4-(4- {3-(2-ammoethoxy)phenyl} -3-methylphenyl)-piperidin- 1 -ylsulphonyI]-2- methylpropanamide hydrochloride The title compound was obtained as a solid (76%), from the hydroxamic acid from preparation 89, following the procedure described in example 12. mp 204-206°C Η nmr (DMSO-d 6 , 400MHz) δ: 1.48 (s, 6H), 1.60 (m, 2H), 1.80 (m, 2H), 2.20 (s, 3H), 2.64 (m, 2H), 3.06 (m, 2H), 3.20 (t, 2H), 3.75 (m, 2H), 4.20 (t, 2H), 6.94 (m, 3H), 7.12 (s, 2H), 7.18 (s, IH), 7.38 (m, 2H), 8.01 (br s, IH), 8.99 (s, IH). LRMS : m z 476 (M+l) + Anal. Found: C, 55.21; H, 6.74; N, 7.83. C 24 H 33 N 3 O 5 S;HCl;0.5H 2 O requires C, 55.32; H, 6.77; N, 8.06%. Example 14 N-Hydroxy 4- {[4-(-4-{6-[2-aminoethoxy]pyridin-2-yl}-3-methylphenyl)piperidin-l- yl] sulphonyl} tetrahydro-2H-pyran-4-carboxamide hydrochloride A saturated solution of hydrogen chloride in dichloromethane (250ml) was added to a solution of the hydroxamic acid from preparation 90 (4.5g, 7.28mmol) in dichloromethane (30ml), and the reaction stirred at room temperature for 3 'A hours. The mixture was cooled in an ice-bath, the resulting precipitate filtered off, and washed with dichloromethane, then ether. The solid was then dried under vacuum at 70°C to afford the title compound (3.1g, 77%). mp 208-210°C Η nmr (DMSO-d β , 400MHz) δ: 1.60 (m, 2H), 1.78 (m, 2H), 1.90 (m, 2H), 2.19 (s, 3H), 2.38 (m, 2H), 2.62 (m, IH), 3.02 (m, 2H), 3.19 (m, 6H), 3.70 (m, 2H), 3.83 (m, 2H), 4.18 (t, 2H), 6.92 (m, 3H), 7.06 (s, 2H), 7.17 (s, IH), 7.35 (m, IH), 9.12 (s, IH). LRMS : m/z 518 (M+l) + Example 15 N-Hydroxy 2-[4-(4-{3-(2-N,N-dimethylaminoethoxy)ρhenyl}-3-methylphenyl)-piperidin-l- ylsulphonyl]-2-methylpropanamide l-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (130mg, 0.68mmol) and l-hydroxy-7- azabenzotriazole (80mg, 0.59mmol) were added to a solution of the acid from preparation 83 (270mg, 0.55mmol) in pyridine (6ml) and dichloromethane (6ml) under a nitrogen atmosphere, and the suspension stined for 30 minutes. N,N-dimethylformamide (5ml), was added, and the reaction warmed to 50°C to obtain a solution. Hydroxylamine hydrochloride (115mg, 1.65mmol) was added and the reaction stirred at room temperature for 18 hours. The reaction mixture was partitioned between ethyl acetate (100ml) and pH 7 buffer solution (30ml), and the phases separated. The organic layer was washed with water (2x30ml), brine (30ml), dried (Na 2 S0 4 ), filtered and evaporated in vacuo. The residue was azeotroped with toluene (3x), and ethyl acetate (2x), and dried under vacuum at 60°C, to afford the title compound as a solid, (180mg, 65%). Η nmr (DMSO-d 6 , 400MHz) δ: 1.48 (s, 6H), 1.60 (m, 2H), 1.78 (m, 2H), 2.19 (s, 9H), 2.60 (m, 3H), 3.03 (m, 2H), 3.76 (m, 2H), 4.05 (t, 2H), 6.80 (m, 2H), 6.86 (m, IH), 7.06 (m, 2H), 7.12 (s, IH), 7.28 (m, IH). LRMS : m/z 504 (M+l) + Anal. Found: C, 60.43; H, 7.50; N, 8.08. C 26 H 37 N 3 O 3 S;0J5H 2 O requires C, 60.38; H, 7.50; N, 8.12%. Example 16 N-Hydroxy 4-{[4-(4-{3-(memyl)aminomethyl}-3-methylphenyl)piperidin-l-yl]sulphonyl}tetrahydro-2H- pyran-4-carboxamide hydrochloride A solution of dichloromethane saturated with hydrogen chloride (20ml) was added to a solution of the hydroxamic acid from preparation 91 (347mg, 0.58mmol) in dichloromethane (10ml), and the solution stirred at room temperature for 4 hours. The reaction mixture was concentrated in vacuo, and the residue triturated with hot methanol/di-isopropyl ether to give the title compound as a white solid, (202mg, 64%). mp 213-214°C Η nmr (DMSO-d 6 , 400MHz) δ: 1.60 (m, 2H), 1.78 (m, 2H), 1.97 (m, 2H), 2.20 (s, 3H), 2.38 (m, 2H), 2.46 (s, 3H), 2.62 (m, IH), 3.01 (m, 2H), 3.18 (m, 2H), 3.70 (m, 2H), 3.82 (m, 2H), 4.12 (s, 2H), 7.10 (m, 3H), 7.35 (s, IH), 7.43 (m, 3H), 9.10 (br, s, IH), 10.92 (s, IH). LRMS : m z 502 (M+l) + Anal. Found: C, 57.16; H, 6.72; N, 7.64. C 26 H 35 N 3 O 5 S;HCl;0.5H 2 O reqires C, 57.08; H, 6.82; N, 7.68%. Example 17 N-Hydroxy 4-{[4-(3-methyl-4-{3-[4-morpholinylmethyl]}phenyl)piperidm-l-yl]sulphonyl}tetrahydro- 2H-pyran-4-carboxamide l-(3-Dimemylaminopropyl)-3-ethylcarbodiimide hydrochloride (265mg, 1.38mmol) and l-hydroxy-7- azabenzotriazole (157mg, 1.15mmol) were added to a solution of the acid from preparation 86 (625mg, 1.15mmol) in pyridine (6ml) and N,N-dimethylformamide (6ml) under a nitrogen atmosphere, and the suspension stirred for 1 hour. Hydroxylamine hydrochloride (210mg, 3.45mmol) was added and the reaction stirred at room temperature for 18 hours. The reaction mixture was partitioned between ethyl acetate and pH 7 buffer solution, the phases separated, and the aqueous layer extracted with ethyl acetate. The combined organic solutions were washed with water, brine, then dried (MgS0 4 ), filtered and concentrated in vacuo. The crude product was purified by column chromatography on silica gel using dichloromethane:methanol (95:5) as eluant, and recrystallised from ethyl acetate to give the desired product as a white solid, (398mg, 62%). mp 177-179°C 'H nmr (DMSO-d 6 , 400MHz) δ: 1.60 (m, 2H), 1.78 (m, 2H), 1.88 (m, 2H), 2.17 (s, 3H), 2.36 (m, 6H), 2.60 (m, IH), 3.00 (m, 2H), 3.19 (m, 2H), 3.46 (s, 2H), 3.53 (m, 4H), 3.70 (m, 2H), 3.81 (m, 2H), 7.06 (m, 7H), 9.10 (s, IH), 10.92 (s, IH). LRMS : m/z 558 (M+l) + Anal. Found: C, 62.15; H, 7.01; N, 7.40. C 29 H 39 N 3 0 6 S requires C, 62.46; H, 7.05; N, 7.53%. Example 18 N-Hydroxy 2-( {4-[4-(3-methoxy- 1 H-pyrazol- 1 -yl)-3-methylphenyl]piperidin- 1 -yl} sulphonyl)-2- methylpropanamide l-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (129mg, 0.67mmol) and l-hydroxy-7- azabenzotriazole (76mg, 0.56mmol) were added to a solution of the acid from preparation 103 (235mg, 0.56mmol) in pyridine (1.5ml) and dichloromethane (3ml) under a nitrogen atmosphere, and the suspension stirred for 30 minutes. Hydroxylamine hydrochloride (78mg, 1.12mmol) was added and the reaction stirred at room temperature for 18 hours. The reaction mixture was poured into ethyl acetate (100ml), washed with pH 7 buffer solution (2x50ml) then dried (MgS0 4 ), filtered and evaporated in vacuo. The residual white solid was recrystallised from hot ethyl acetate, to afford the title compound as a white solid, (156mg, 64%). mp 172-173°C 'H nmr (CD 3 OD, 400MHz) δ: 1.58 (s, 6H), 1.74 (m, 2H), 1.82 (m, 2H), 2.20 (s, 3H), 2.70 (m, IH), 3.09 (m, 2H), 3.87 (m, 5H), 5.84 (s, IH), 7.16 (m, IH), 7.20 (m, 2H), 7.48 (s, IH). Anal. Found: C, 55.04; H, 6.42; N, 12.77. C 20 H 28 N 4 O 5 S requires C, 55.03; H, 6.47; N, 12.83%. Example 19 N-Hydroxy 2-[(4-{4-[3-(2-hydroxyethoxy)-lH-pyrazol-l-yl]-3-methylphenyl}piperidin-l-yl)sulphonyl]- 2-methylpropanamide Pyridine (6ml) was added to a suspension of the acid from preparation 104 (325mg, 0.72mmol) in dichloromethane (6ml), and the solution purged with nitrogen. Chlorotrimethylsilane (858mg, 0.79mmol) was added, the solution stined for an hour, then l-hydroxy-7-azabenzotnazole (98mg, 0.72mmol) was added, followed by l-(3-dιmethylamιnopropyl)-3-ethylcarbodιιmιde hydrochloride (166.8mg, 0.87mmol), and the solution was stined for a further hour. Hydroxylamme hydrochloride (150mg, 2.16mmol) was then added and the reaction stured at room temperature for 17 hours The reaction was partitioned between ethyl acetate and pH 7 buffer solution, and the pH of the mixture carefully adjusted to 3 usmg hydrochloric acid (2N) The layers were separated, the organic phase dned (MgS0 4 ), filtered and evaporated in vacuo, and the residue tnturated with ether. The resultmg white solid was filtered, then dissolved m a solution of acetic acid (10ml), water (10ml), and methanol (10ml), and this mixture stined at room temperature for 45 mmutes. The solution was poured mto pH 7 buffer (300ml), extracted with ethyl acetate (3x100ml), and the combined organic extracts dned (MgS0 4 ), filtered and concentrated in vacuo. The residue was azeotroped with toluene and ethyl acetate, and tnturated several tunes with ether to give the title compound as a white solid, (141mg, 42%). Η nmr (D SO-dβ, 400MHz) δ: 1.43 (s, 6H), 1.59 (m, 2H), 1.77 (m, 2H), 2.19 (s, 3H), 2.62 (m, IH), 3.00 (m, 2H), 3.66 (m, 4H), 4.05 (t, 2H), 4.72 (br, t, IH), 5.84 (s, IH), 7.15 (m, IH), 7.19 (m, 2H), 7.72 (s, IH), 8.90 (s, IH), 10.66 (s, IH). Anal. Fond: C, 53.85; H, 6.49; N, 11.86. C^H JO S requires C, 54.06; H, 6.48; N, 12.01%. Example 20 N-Hydroxy 2-methyl-2-( {4-[3-methyl-4-( 1 ,3-thιazol-2-yl)phenyl]pιpendm- 1 -yl} sulphonyl)propanamide The title compound was prepared from the acid from preparation 105, followmg the procedure described m example 18. The crude product was crystallised from a minimum volume of methanol to give the desired product as a white solid, (58mg, 35%). mp 199-201°C ! H nmr (DMSO-d 6 , 400MHz) δ: 1.45 (s, 6H), 1.60 (m, 2H), 2.44 (s, 3H), 2.65 (m, IH), 3.01 (m, 2H), 3.14 (s, 2H), 3.72 (m, 2H), 7.18 (d, IH), 7.20 (s, IH), 7.61 (d, IH), 7.75 (s, IH), 7.90 (s, IH), 8.82 (br, s, IH), 10.60 (s, IH). Anal. Found: C, 53.51; H, 5.92; N, 9.75. C l9 H 25 N 3 0 4 S 2 requires C, 53.88; H, 5.95; N, 9.92%. Example 21 (lα,3α,4α)-/V,3,4-trihydroxy-l-[(4-{4-[6-(2-hydroxyethoxy)ρyridin-2-yl]-3-methylphenyl}piperidin-l- yl)sulfonyl]cyclopentanecarboxamide Hydrogen chloride gas was bubbled through a solution of the tert-butyl ether from preparation 121 (260mg, 0.412mmol) in trifluoroacetic acid (10ml) and dichloromethane (10ml) for 5 minutes, and the reaction was stined for 5 '/- hours at ambient temperature. The reaction mixture was evaporated in vacuo and the resulting oil azeotroped with toluene (x2) before partitioning between ethyl acetate (50ml) and pH7 phosphate buffer solution (40ml). The organic layer was separated and the aqueous layer was extracted with ethyl acetate (2x50ml). The combined organic extracts were dried (Na 2 S0 4 ), filtered and evaporated in vacuo. The resulting solid, which contained some of the starting compound, was resubmitted to the reaction conditions. After 5 hours at ambient temperature nitrogen gas was bubbled through the reaction mixture for 15 minutes. The reaction mixture was then evaporated in vacuo and the resulting oil azeotroped with toluene (x2) before partitioning between ethyl acetate (50ml) and pH7 phosphate buffer solution (40ml). The organic layer was separated and the aqueous layer extracted with ethyl acetate (2x50ml). The combined organic extracts were dried (Na 2 S0 4 ), filtered and evaporated in vacuo. The resulting solid was purified by column chromatography on silica gel using dichloromethane/methanol (98:2 to 93:7) as eluant. The title compound was isolated as a white solid (30mg, 15%). Η nmr (DMSO-d-s, 400MHz) δ: 1.59 (m, 2H), 1.76 (m, 2H), 2.22 (m, 2H), 2.32 (s, 3H), 2.39 (m, 2H), 2.60 (m, IH), 2.99 (t, 2H), 3.64 (m, 4H), 3.90 (s, 2H), 4.23 (m, 2H), 4.54 (s, 2H), 4.75 (t, IH), 6.72 (d, IH), 7.03 (d, IH), 7.15 (m, 2H), 7.31 (d, IH), 7.73 (t, IH), 8.95 (s, IH), 10.69 (s, IH). LRMS :m z 536 (M+l) + . WO 00/74681 g PCT/IB0O/O0667 mp 215-218°C Anal Found C, 49 73, H, 5 67, N, 6 45 C 25 H 33 N 3 0 8 S,TFA, 0 5MeOH requires C, 49 62, H, 5 45, N, 6.31%. Example 22 ( 1 α,3α,4α)- 1 -( {4-[4-(6-ethoxypyπdm-2-yl)-3-methylphenyl]pιpeπdιn- 1 -yl} sulfonyl)-N3,4- tnhydroxycyclopentanecarboxamide 2N Hydrochloπc acid (2ml) was added to a solution of the dioxolane from preparation 122 m dioxan (2ml) and tetrahydrofuran (2ml) and the reaction mixture was stined at ambient temperature for 18 hours The reaction mixture was evaporated m vacuo and the resultmg solid partitioned between pH7 phosphate buffer solution (20ml) and ethyl acetate (20ml). The aqueous layer was extracted with ethyl acetate (2x20ml) and the combined organic extracts were dπed (Na 2 S0 4 ) filtered and concentrated m vacuo. The resulting solid was recrystahsed from ethyl acetate to afford the title compound as a white solid (95mg, 70%). 'H nmr (DMSO-d β , 400MHz) δ: 1.25 (t, 3H), 1.58 (m, 2H), 1.76 (m, 2H), 2.22 (m, 2H), 2.35 (s, 3H), 2.38 (m, 2H), 2.60 (m, IH), 2.99 (t, 2H), 3.66 (d, 2H), 3.85 (s, 2H), 4.25 (q, 2H), 4.61 (s, 2H), 6.71 (d, IH), 7.03 (d, IH), 7.12 (m, 2H), 7.31 (d, IH), 7.72 (t, IH), 9.00 (s, IH), 10.78 (s, IH) LRMS :m/z 520 (M+l) + . mp 204-205°C Anal. Found. C, 57.42, H, 6.36, N, 7.98. C^N^S; 0.25 H 2 0 requires C, 57.29, H, 6.44; N, 8.02%. Example 23 ( 1 α,3 β,4 β)- 1 -( {4-[4-(6-ethoxypyndιn-2-yl)-3-methylphenyl]pιpendιn- 1 -yl} sulfonyl)-N3,4- tnhydroxycyclopentanecarboxamide The title compound was prepared from the dioxolane from preparation 123 in a similar procedure to that described in example 22. This afforded the title compound as a white solid (50mg, 55%). Η nmr (DMSO-d , 400MHz) δ: 1.27 (t, 3H), 1.62 (m, 2H), 1.78 (m, 2H), 2.09 (m, 2H), 2.35 (s, 3H), 2.61 (m, IH), 2.74 (m, 2H), 3.01 (t, 2H), 3.69 (m, 4H), 4.29 (q, 2H), 4.49 (s, 2H), 6.69 (d, IH), 7.02 (d, IH), 7.12 (m, 2H), 7.31 (d, IH), 7.73 (t, IH), 8.92 (s, IH), 10.71 (s, IH). LRMS :m/z 520 (M+l) + . mp 196-197°C Anal. Found: C, 56.83; H, 6.32; N, 7.83. C 25 H 33 N 3 0 7 S; 0.5 H 2 0 requires C, 56.80; H, 6.48; N, 7.95%. Example 24 (lα,3α,4α)-N3,4-trihydroxy-l-{4-[4-(3-methoxyphenyl)-3-methylphenyl]piperidin-l- ylsulfonyl}cyclopentanecarboxamide 2N Hydrochloric acid (2ml) was added to a solution of the dioxolane from preparation 124 in dioxan (3ml) and tetrahydrofuran (2ml) and the reaction mixture was stined at ambient temperature for 4 hours. The reaction mixture was evaporated in vacuo and the resulting solid was partitioned between water (20ml) and ethyl acetate (20ml). The aqueous layer was extracted with ethyl acetate (2x20ml) and the combined organic extracts were dried (Na 2 S0 4 ) ι filtered and concentrated in vacuo. The resulting solid was recrystalised from ethyl acetate to afford the title compound as a white solid (60mg, 46%). Η nmr (DMSO-d 6 , 400MHz) δ: 1.58 (m, 2H), 1.76 (m, 2H), 2.19 (s, 3H), 2.24 (m, 2H), 2.38 (m, 2H), 2.60 (m, IH), 2.99 (t, 2H), 3.71 (m, 5H), 3.79 (s, 2H), 4.54 (s, 2H), 6.82 (m, 3H), 7.11 (m, 3H), 7.32 (t, IH), 8.97 (s, IH), 10.70 (s, IH). LRMS :m/z 527 (M+23) + . mp 201-202°C Anal. Found: C, 58.85; H, 6.36; N, 5.51. C 25 H 32 N 2 0 7 S; 0.25 H 2 0 requires C, 58.98; H, 6.43; N, 5.50%. Example 25 ( 1 ,3 β,4 β)-/V,3 ,4-trihydroxy- 1 - {4-[4-(3-methoxyphenyl)-3-methylphenyl]piperidin- 1 - ylsulfonyl}cyclopentanecarboxamide HO OH The title compound was prepared from the dioxolane from preparation 125 in a similar procedure to that described in example 24. This afforded the title compound as a white solid (55mg, 50%). Η nmr (DMSOA, 400MHz) δ: 1.59 (m, 2H), 1.76 (m, 2H), 2.17 (m, 2H), 2.19 (s, 3H), 2.60 (m, IH), 2.71 (m, 2H), 2.99 (t, 2H), 3.70 (m, 7H), 4.61 (s, 2H), 6.82 (m, 3H), 7.12 (m, 3H), 7.32 (t, IH), 9.00 (s, IH), 10.82 (s, IH). LRMS :m z 503 (M-l)\ mp 188-189°C Anal. Found: C, 58.97; H, 6.50; N, 5.49. C 25 H 32 N 2 0 7 S; 0.25 H 2 0 requires C, 58.98; H, 6.43; N, 5.50%. Preparation 1 2-[2-(Benzyloxy)ethoxy]-6-bromopyridine Sodium hydride (900mg, 60% dispersion in mineral oil, 22 5mmol) was added portionwise to an ice- cold solution of 2-(benzyloxy)ethanol (3 Og, 20 Ommol) in toluene (100ml), and the solution stined for 30 minutes 2,6-Dιbromopyndme (4 75g, 20 Ommol) was added, and the reaction heated under reflux for 2 hours The cooled mixture was diluted with water (100ml), and extracted with ethyl acetate (3x100ml) The combined organic extracts were dried (MgS0 4 ), filtered and evaporated m vacuo to give the title compound as a yellow oil, (quantitative) Η nmr (CDC1 3 , 300MHz) δ 3 82 (t, 2H), 4 52 (t, 2H), 4 62 (s, 2H), 6 75 (d, IH), 7 05 (d, IH), 7 22-7 46 (m, 6H) Preparation 2 2-Bromo-6- { [(4R)-2,2-dιmethyl- 1 ,3-dιoxolan-4-yl]methoxy } pyndine Sodium hydride (1 62g, 60% dispersion in mineral oil, 40 5mmol) was added portionwise to an ice- cooled solution of (R)-(-)-l,2-0-ιsopropyhdeneglycerol (4 86g, 36 8mmol) in toluene (100ml), and once addition was complete, the solution was allowed to warm to room temperature and stirred for 30 mmutes 2,6-Dιbromopyπdιne (8 72g, 36 8mmol) was added, and the reaction heated under reflux for 5 hours The cooled mixture was diluted with water, the layers separated, and the aqueous phase extracted with ether The combmed organic extracts were dπed (MgS0 4 ), filtered and evaporated m vacuo to afford the title compound as a yellow oil (quantitative) 'H nmr (CDC1 3 , 300MHz) δ 1 39 (s, 3H), 1 45 (s, 3H), 3 83 (dd, IH), 4 16 (dd, IH), 4 37 (m, 2H), 4 46 (m, IH), 6 75 (d, IH), 7 06 (d, IH), 7 40 (dd, IH) Preparation 3 2-Bromo-6-{[(4S)-2,2-dnnethyl-l,3-dιoxolan-4-yl]methoxy}pyπdme The title compound was obtamed as a yellow oil (quantitative), from (S)-(-)- 1,2-0- lsopropyhdeneglycerol and 2,6-dιbromoρyndιne, following the procedure descnbed m preparation 2 Η nmr (CDC1 3 , 300MHz) δ 1 40 (s, 3H), 1 45 (s, 3H), 3 83 (dd, IH), 4 16 (dd, IH), 4 37 (m, 2H), 4 48 (m, IH), 6 76 (d, IH), 7 06 (d, IH), 7 41 (m dd, IH) Preparation 4 2-[2-(Benzyloxy)ethoxy]-6-(tiibutylstannyl)pyridine n-Butyllithium (13.8ml, 1.6M solution in hexanes, 22.0mmol) was added dropwise to a cooled (-78 C C) solution of the bromide from preparation 1 (20. Ommol) in anydrous THF (100ml), so as to maintain the internal temperature <-70°C, and the solution stined for 20 minutes. Tri-n-butyltin chloride (6.0ml, 22. Ommol) was added slowly to maintain the temperature <-70°C, and the reaction then allowed to warm to room temperature over 1 hour. The reaction was diluted with water, the mixture extracted with Et 2 0 (2x100ml), and the combined organic extracts dried (MgS0 4 ), filtered and evaporated in vacuo. The residue was purified by column chromatography on silica gel using pentane:Et 2 0 (98:2) as eluant, to afford the title compound as a colourless oil, (7.0g, 67%). 'H nmr (CDC1 3 , 300MHz) δ: 0.88 (t, 9H), 1.06 (m, 6H), 1.35 (m, 6H), 1.58 (m, 6H), 3.83 (t, 2H), 4.56 (t, 2H), 4.62 (s, 2H), 6.61 (d, IH), 6.99 (d, IH), 7.24-7.40 (m, 6H). Preparation 5 2- { t(4R)-2,2-Dimethyl- 1 ,3-dioxolan-4-yl]methoxy } -6-(tributylstannyl)pyridine The title compound was prepared as an oil (quantitative) from the bromide of preparation 2, using a similar procedure to that described in preparation 4. 'H nmr (CDC1 3 , 300MHz) δ: 0.88 (t, 9H), 1.06 (t, 6H), 1.25-1.40 (m, 9H), 1.45 (s, 3H), 1.50-1.70 (m, 6H), 3.83 (dd, IH), 4.15 (dd, IH), 4.40 (m, 2H), 4.52 (m, IH), 6.60 (d, IH), 7.00 (d, IH), 7.40 (dd, IH). Preparation 6 2-{[(4S)-2,2-Dimethyl-l,3-dioxolan-4-yl]methoxy}-6-(tributylstannyl)pyridine The title compound was obtained as a colourless oil (71%), from the bromide from preparation 3, following a similar procedure to that described in preparation 5. 'H nmr (CDC1 3( 300MHz) δ: 0.89 (t, 9H), 1.07 (t, 6H), 1.35 (m, 6H), 1.40 (s, 3H), 1.48 (s, 3H), 1.58 (m, 6H), 3.83 (dd, IH), 4.16 (dd, IH), 4.40 (m, 2H), 4.52 (m, IH), 6.60 (d, IH), 7.00 (d, IH), 7.40 (dd, IH). Preparation 7 3 -Bromo- 1 -(tert-butoxy)benzene Condensed isobutylene (100ml) was added via a dry ice/acetone cold finger, to dichloromethane (70ml) at -30°C, followed by a solution of 3-bromophenol (21.5g, 125mmol) in dichloromethane (30ml). Trifluoromethanesulphonic acid (1.5g, 10. Ommol) was added dropwise, the reaction cooled to -75°C, and stined for 2 hours. Triethylamine (1.4ml, 10. Ommol) was then added, the solution allowed to warm to room temperature and then concentrated in vacuo to remove the isobutylene. The remaining solution was washed with water, dried (Na 2 S0 4 ), filtered and evaporated to give the desired product as a pale yellow oil, (33g, slightly impure). 'H nmr (CDC1 3 , 400MHz) δ: 1.37 (s, 9H), 6.89 (d, IH), 7.04-7.20 (m, 3H). Preparation 8 3-(tert-Butoxy)-phenylboronic acid n-Butyllithium (40ml, 2.5M in hexanes, lOOmmol) was added dropwise to a cooled (-78°C) solution of the bromide from preparation 7 (23.9g, 90mmol) in tetrahydrofuran (300ml), so as to maintain the temperature below -70°C. The resulting solution was stined for 1 hour, and triisopropyl borate (30.6ml, 135mmol) was added dropwise over 10 minutes. The reaction was allowed to warm to room temperature, diluted with ether (150ml) then extracted with sodium hydroxide solution (IN). The combined aqueous layers were washed with ether and then re-acidified to pH 2 using hydrochloric acid (2N). This aqueous mixture was extracted with dichloromethane (3x200ml), the combined organic extracts dried (Na 2 S0 4 ), filtered and concentrated in vacuo. The resulting white solid was stined vigorously in pentane, filtered (twice) then dried under vacuum to give the title compound as a white solid, (13.1g, 75%). Η nmr (CDC1 3 , 400MHz) δ: 1.39 (s, 9H), 7.19 (m, IH), 7.37 (m, IH), 7.79 (m, IH), 7.88 (m, IH). Preparation 9 l-Bromo-3-(2,2-diethoxyethoxy)benzene A mixture of potassium carbonate (1 5g, 10 9mmol), 3-bromophenol (1.73g, 10. Ommol) and bromoacetaldehyde diethyl acetal (1.5ml, 9 67mmol) m dimethylsulphoxide (10ml) was heated at 160°C for 1 ' hours The cooled reaction was partitioned between water (50ml) and ethyl acetate (100ml), and the phases separated The aqueous layer was extracted with ethyl acetate (50ml), the combmed organic solutions washed consecutively with IN sodium hydroxide solution, water (2x), brine and then dπed (Na 2 S0 4 ), filtered and evaporated m vacuo The residue was punfied by medium pressure column chromatography on silica gel using an elution gradient of ether pentane (0 100 to 5:95) to afford the title compound (2 01 g, 72%) Η nmr (CDC1 3 , 400MHz) δ 1 22 (t, 6H), 3 60 (m, 2H), 3 75 (m, 2H), 3 97 (d, 2H), 4.80 (t, IH), 6 82 (d, IH), 7.07 (m, 3H) Preparation 10 3-(2,2-Dιethoxyethoxy)phenylboronιc acid n-Butylhthium (18.5ml, 2.5M m hexanes, 46.25mmol) was added dropwise to a cooled (-78°C) solution of the bromide from preparation 9 (11.4g, 39.6mmol) in anhydrous tetrahydrofuran (100ml), so as to maintain the internal temperature <-70°C. This solution was stined for 1 hour, then tnisopropyl borate (1.13g, ό.Ommol) added slowly, and the reaction allowed to warm to room temperature over 3 hours. The mixture was cooled m an ice-bath, acidified to pH 4 usmg 2N hydrochloπc acid, and quickly extracted with ethyl acetate (2x500ml). The combmed organic extracts were washed with water and brine, dπed (Na 2 S0 4 ), filtered and evaporated in vacuo. The residual oil was puπfied by medium pressure column chromatography on silica gel usmg an elution gradient of etheπpentane (0:100 to 50.50) to afford the title compound (8.24g, 82%). Η nmr (DMSO-c^, 400MHz) δ. 1.14 (t, 6H), 3 58 (m, 2H), 3.66 (m, 2H), 3.94 (d, 2H), 4.80 (t, IH), 6.98 (m, IH), 7.22 (m, IH), 7.37 (m, 2H), 8.00 (s, 2H). Preparation 11 l-Methylsulphonyl-pιpeπdm-4-one ethylene ketal Methanesulphonyl chloride (24.8g, 0.217mol) was added dropwise to a solution of 4-piperidone ethylene ketal (28.2g, 0.197mol) and triethylamine (30.2ml, 0.217mol) in ether (280ml), and the reaction stined at room temperature for 3 hours. The mixture was washed consecutively with water (2x), hydrochloric acid (IN), and saturated sodium bicarbonate solution, dried (MgS0 4 ), filtered and evaporated in vacuo. The residue was triturated with hexane, filtered and dried to give the desired product as an off-white solid (41.6g, 95%). mp 107-109°C •H nmr (CDC1 3 , 400MHz) δ: 1.78 (m, 4H), 2.75 (s, 3H), 3.32 (m, 4H), 3.92 (s, 4H). Anal. Found: C, 43.23; H, 6.85; N, 6.23. C 8 H I5 N0 4 S requires C, 43.42; H, 6.83; N, 6.33%. Preparation 12 l-Isopropylsulphonyl-piperidin-4-one ethylene ketal Isopropylsulphonyl chloride (5.6ml, 50mmol) was added dropwise to an ice-cooled solution of 4- piperidone ethylene ketal (6.4ml, 50mmol) and triethylamine (7.7ml, 55mmol) in dichloromethane (100ml), and the reaction stined at room temperature for 3 hours. The mixture was washed with water (2x), dried (MgS0 4 ), filtered and evaporated in vacuo. The residue was crystallised from ether/pentane to afford the title compound as a solid, (10.55g, 85%). mp 66-67°C Η nmr (CDC1 3 , 400MHz) δ: 1.34 (d, 6H), 1.77 (m, 4H), 3.18 (m, IH), 3.43 (m, 4H), 3.98 (s, 4H). Anal. Found: C, 48.19; H, 7.74; N, 5.50. C 10 H„NO 4 S requires C, 48.15; H, 7.75; N, 5.56%. Preparation 13 Methyl 2-(l,4-dioxa-8-azaspiro[4.5]dec-8-ylsulphonyl)acetate X » α υ ---- i J o ..N. Me . O- XJ ?, ' * . o Potassium tert-butoxide (24.6g, 219mmol) was added portionwise to a solution of the ethylene ketal from preparation 11 (32.3g, 146mmol) and dimethyl carbonate (61ml, 730mmol) in tetrahydrofuan (200ml), and once addition was complete, the reaction was stined at room temperature overnight under a nitrogen atmosphere. The reaction was poured into a mixture of hydrochloric acid (IN) and ether and the layers separated. The aqueous layer was extracted with ethyl acetate, the combined organic solutions washed with brine, dried (MgS0 4 ), filtered and evaporated in vacuo. The residue was suspended in di-isopropyl ether, the mixture heated to reflux, cooled, and filtered, to afford the title compound as a solid, (26.7g, 65%). Η nmr (CDC1 3 , 400MHz) δ: 1.77 (m, 4H), 3.42 (m, 4H), 3.78 (s, 3H), 3.92 (s, 2H), 3.95 (s, 4H). Anal. Found: C, 42.69; H, 6.16; N, 4.93. C 10 H 17 NO 6 S requires C, 43.00; H, 6.14; N, 5.02%. Preparation 14 Methyl 2-( 1 ,4-dioxa-8-azaspiro[4.5]dec-8-ylsulphonyl)-2-methylpropanoate N-Butyl lithium (28ml, 1.6M in hexanes, 44.1mmol) was added dropwise to a cooled (-78°C) solution of tlie sulphonamide from preparation 12 (10g, 40.1mmol) in tetrahydrofuran (100ml), so as to maintain a temperature below -45°C. Once addition was complete the solution was allowed to warm to 0°C, and then recooled to -78°C. Methyl chloroformate (3.7ml, 48.1mmol) was added dropwise so as to maintain the temperature below -45°C, the reaction stined for 30 minutes, then allowed to warm to room temperature. The reaction mixture was partitioned between ethyl acetate and water, and the layers separated. The organic phase was washed with water, dried (MgS0 4 ), filtered and evaporated in vacuo. The crude product was triturated with ether to give the title compound as a solid, (9.88g, 80%). Η nmr (CDC1 3 , 400MHz) δ: 1.60 (s, 6H), 1.76 (m, 4H), 3.48 (m, 4H), 3.79 (s, 3H), 3.98 (s, 4H). Anal. Found: C, 46.80; H, 6.87; N, 4.49. C 12 H 2l N0 6 S requires C, 46.89; H, 6.89; N, 4.56%. Preparation 15 Methyl 4-(l,4-dioxa-8-azaspiro[4.5]dec-8-ylsulphonyl)tetrahydro-2H-pyran-4-carboxylate Sodium hydride (880mg, 60% dispersion in mineral oil, 22mmol) was added to a solution of the sulphonamide from preparation 11 (2.2 lg, 1 Ommol) and dimethyl carbonate (4.2ml, 50mmol) in dry toluene (40ml), and the mixture heated at 90°C for 90 minutes. Tic analysis showed starting material present, so methanol (20?1) was added, and the reaction stined at 90°C ovemight. l-Methyl-2- pynolidinone (10ml) and bis(2-bromoethyl)ether (1.63ml, 13mmol) were added, and the reaction sti ed for a further 20 hours at 90°C, and at room temperature for 3 days. The reaction mixture was partititoned between IN citric acid solution and ether, and the layers separated. The organic phase was washed with water, dried (MgS0 4 ), filtered and evaporated in vacuo. The residue was triturated with ether to give the title compound as a white solid, (1.05g, 30%). Alternative method Potassium tert-butoxide (220ml, 1M in tetrahydrofuran, 220mmol) was added dropwise to a solution of the acetate from preparation 13 (27.9g, lOOmmol) and bis(2-bromoethyl)ether (16.3ml, 130mmol) in tetrahydrofuran (200ml) and l-methyl-2-pynolidinone (20ml), and the reaction stined at room temperature ovemight. Tic analysis showed starting material remaining, so tetrabutylammonium iodide (3.7g, 1 Ommol) and sodium hydride (2.0g, 60% dispersion in mineral oil, 50mmol) were added, and the reaction stirred for a further 72 hours. Additional l-methyl-2-pyπolidinone (100ml), sodium hydride (4.0g, 60% dispersion in mineral oil, lOOmmol) and bis(2-bromoethyl)ether (12.6ml, lOOmmol) were added, and the reaction continued for a further 24 hours. The reaction was poured into a mixture of ether and 10% citric acid solution, and the layers separated. The aqueous phase was extracted with ether, the combined organic solutions washed with water, dried (MgS0 4 ), filtered and evaporated in vacuo.The residue was suspended in ether, the mixture heated to reflux, cooled and the resulting precipitate filtered, washed with ether and dried to give the title compound, (7.2g, 21%). 'H nmr (CDC1 3 , 400MHz) δ: 1.70 (m, 4H), 2.16 (m, 2H), 2.35 (m, 2H), 3.24 (m, 2H), 3.41 (m, 4H), 3.80 (s, 3H), 3.94 (m, 6H). LRMS : m/z 372 (M+23) + Preparation 16 Methyl 4-(4-oxo-piperidin- 1 -ylsulphonyl)tetrahydro-2H-pyran-4-carboxylate Hydrochloric acid (20ml, IN) was added to a solution of the ethylene ketal from preparation 15 (7.1g, 20.3mmol) in acetone (20ml) and 1,4-dioxan (20ml), and the reaction stined at 60°C for 6 hours, and then left at room temperature overnight The reaction was neutralised by adding sodium bicarbonate portionwise, and this mixture concentrated in vacuo The residue was diluted with water, then extracted with ethyl acetate (3x) The combined organic extracts were dned (MgS0 4 ), filtered and evaporated m vacuo.The crude product was tnturated with ether/di-isopropyl ether, to give the desired product as a solid (4 lg, 66%) mp 158-160°C 'H nmr (CDC1 3 , 400MHz) δ- 2.18 (m, 2H), 2.38 (m, 2H), 2 48 (m, 4H), 3 26 (m, 2H), 3.60 (br, m, 4H), 3.82 (s, 3H), 3.98 (m, 2H) Anal. Found. C, 47.14; H, 6.28, N, 4.54 C 12 H 19 N0 6 S requires C, 47.20, H, 6 27; N, 4 59%. Preparation 17 Methyl 2-methyl-2-(4-oxo-pιpendm- 1 -ylsulρhonyl)propanoate The title compound was obtamed as a solid (98%) after tnturation with pentane from the ethylene ketal from preparation 14, followmg a similar method to that descπbed m preparation 16. Η nmr (CDC1 3 , 400MHz) δ: 1.67 (s, 6H), 2.57 (m, 4H), 3.68 (m, 4H), 3.80 (s, 3H). Anal. Found. C, 45.51; H, 6.52; N, 5.14. C l0 H 17 NO 5 S requires C, 45.61; H, 6.51; N, 5.32%. Preparation 18 tert-Butyl 4-[4-(4-bromo-3-methylphenyl)-4-hydroxypιperidme- 1 -carboxylate A 2.5M solution of n-butyl lithium in hexane (38ml, 94mmol) was added over about 10 minutes to a stined mixture of 2-bromo-5-iodo-toluene (28g, 94mmol) in anhydrous ether (500ml) under nitiogen, at about -75°C. After a further 15 minutes, a solution of t-butyl 4-oxopiperidine-l -carboxylate (17 g, 85 mmol) in anhydrous tetrahydrofuran (50 ml) was added at such a rate that the reaction temperature was maintained below -60°C. The reaction mixture was stined at about -75°C for 1 hour, and allowed to warm to 0°C and quenched with aqueous ammonium chloride solution. The organic phase was separated, washed with water, dried (MgS0 4 ), filtered and evaporated in vacuo. The residue was dissolved in pentane and cooled to 0 C C to crystallise the title compound, which was collected by filtration as a colourless solid (20.1 g, 64%). m.p. 102-103°C. Η nmr (CDC1 3 ) δ: 1.48 (s, 9H), 1.51 (s, IH), 1.70 (d, 2H), 1.96 (m, 2H), 2.40 (s, 3H), 3.22 (t, 2H), 4.02 (m, 2H), 7.15 (dd, IH), 7.36 (d, IH), 7.50 (d, IH). LRMS :m z 369/371 (M+l) + Anal. Found: C, 55.14; H, 6.58; N, 3.76. C 17 H 24 BrN0 3 requires C, 55.14; H, 6.53; N, 3.78%. Preparation 19 4-(4-Bromo-3-methylphenyl)- 1 ,2,3,6-tetrahydropyridine Trifluoroacetic acid (100ml) was added to a stined solution of the bromide from preparation 18 (20g, 54mmol) in dichloromethane (100 ml) at room temperature. After a further 18 hours, the reaction mixture was evaporated in vacuo and the residue basified with 2M aqueous sodium hydroxide solution to pH>12. The resulting mixture was extracted with ether, the combined extracts washed with water, dried (MgS0 4 ), filtered and evaporated under reduced pressure to yield the title compound as a low melting solid, (13.6 g, 100%). Η nmr (CDC1 3 ) δ: 1.60 (br, s, IH), 2.40 (m, 5H), 3.10 (t, 2H), 3.52 (m, 2H), 6.10 (br, s, IH), 7.05 (dd, IH), 7.22 (d, IH), 7.46 (d, IH). LRMS :m/z 251/253 (M+l) + . Alternative Method - Para-toluenesulphonic acid (10.27g, 54mmol) was added to a stined solution of the bromide from preparation 18 (lOg, 27mmol) in toluene (130ml) at room temperature. The gelatinous mixture was heated to reflux in a Dean-Stark apparatus for 90 minutes, and then cooled to room temperature which resulted in a thick white precipitate. The mixture was basified with 2M sodium hydroxide solution, and extracted with ethyl acetate (3x), then the combined extracts were washed with water, dried (MgS0 4 ) and evaporated under reduced pressure to yield the title as a low melting solid, (6.8 g, 100%). Preparation 20 4-(4-Bromo-3-methylphenyl)-l-methylsulphonyl-l,2,3,6-tetrahydropyridine Methanesulphonyl chloride (17.5ml, 227mmol) was added dropwise to an ice-cooled solution of triethylamine (34.4ml, 247mmol) and the amine from preparation 19 (51.8g, 206mmol) in dichloromethane (400ml), and die reaction then stirred at room temperature for 1 hour. Tic analysis showed starting material remaining, so additional methanesulphonyl chloride (1.75ml, 22.7mmol) and triethylamine (5ml, 35.9mmol) were added, and stirring continued for a further hour. The reaction was diluted with hydrochloric acid (200ml, 2N) and water (300ml), and die phases separated. The aqueous layer was extracted with dichloromethane (2x250ml) the combined organic extracts washed with brine (200ml), dried (MgS0 4 ), filtered and concentrated in vacuo. The residual solid was triturated with isopropyl ether, filtered and dried to afford the title compound as a pale yellow solid, (65. lg, 96%). 'H nmr (CDC1 3> 300MHz) δ: 2.40 (s, 3H), 2.62 (m, 2H), 2.85 (s, 3H), 3.54 (m, 2H), 3.95 (m, 2H), 6.04 (m, IH), 7.04 (dd, IH), 7.21 (m, IH), 7.50 (d, IH). LRMS m/z 347, 349 (M+18) + Preparation 21 Methyl 2-[4-(4-bromo-3-methylphenyl)-l,2,3,6-tetrahydropyridin-l-ylsulphonyl]acetate WO 00/74681 61 PCT/IBO0/OO667 N,0-Bis(trimethylsiIyI)acetamide (0.9ml, 4. Ommol) was added to a stined solution of the amine from preparation 19 (2.0g, 7.9mmol) in anhydrous tetiahydrofuran (40ml), under nitrogen, at room temperature. A solution of methyl chlorosulphonylacetate (1.64g, 9.5mmol) in anhydrous tetiahydrofuran (15 ml) was added and the reaction mixture stined at room temperature for 18 hours. The resulting mixture was evaporated in vacuo, and partitioned between ethyl acetate and aqueous sodium bicarbonate solution. The organic layer was separated and washed with water, dried (MgS0 4 ), filtered and evaporated in vacuo. The residue was purified by column chromatography on silica gel, using dichloromethane as eluant, followed by crystallisation from diisopropyl ether, to give the title compound as a colourless solid, (1.65 g, 55%). m.p. 110-112°C. 'H nmr (CDC1 3 ) δ: 2.40 (s, 3H), 2.60 (m, 2H), 3.60 (t, 2H), 3.80 (s, 3H), 4.01 (s, 2H), 4.07 (m, 2H), 6.02 (br, s,lH), 7.02 (dd, IH), 7.21 (d, IH), 7.50 (d, IH). LRMS :m/z 404/406 (M+18) + Anal. Found: C, 46.32; H, 4.62; N, 3.55. C 15 H 18 BrN0 4 S requires C, 46.40; H, 4.67; N, 3.61%. Preparation 22 Methyl 2-[4-(4-bromo-3-methylphenyl)- 1 ,2,3,6-tetrahydropyridin- 1 -ylsulphonyl]-2-methyl-propanoate Iodomethane (2ml, 32.1 mmol) was added to a stined mixture of the acetate from preparation 21 (5g, 12.9mmol) and potassium carbonate (5.4g, 39.1mmol), in anhydrous dimethylsulfoxide (50ml), under nitrogen, at room temperature. After 24 hours the reaction mixture was partitioned between ether and water, separated, and the organic layer was washed with water, dried (MgS0 4 ), filtered and evaporated in vacuo. The residue was purified by flash chromatography, using diethyl etheπpentane (40:60 to 100:0) as eluant, followed by crystallisation from diisopropyl ether, to give the title compound as a colourless solid, (4.7 g, 87%). m.p. 100-101°C. Η nmr (CDC1 3 ) δ: 1.67 (s, 6H), 2.40 (s, 3H), 2.58 (m, 2H), 3.60 (t, 2H), 3.80 (s, 3H), 4.08 (m, 2H), 6.00 (br, s, IH), 7.03 (dd, IH), 7.21 (d, IH), 7.49 (d, IH). Anal. Found: C, 49.00; H, 5.33; N, 3.28. C, 7 H 22 BrN0 4 S requires C, 49.04; H, 5.33; N, 3.36%. Preparation 23 Methyl 4-[4-(4-bromo-3-methylphenyl)-l,2,3,6-tetrahydropyridin-l-ylsulphonyl]tetrahydro-2H-pyran-4- carboxylate Bis-2-iodoethyl ether (3.9g, 12.0mmol) was added to a stined mixture of the acetate from preparation 21 (3.6 g, 9.3mmol) and potassium carbonate (3.8g, 27.8mmol), in anhydrous dimethylsulfoxide (50ml), under nitrogen, at room temperature. After 18 hours the reaction mixture was partitioned between diethyl ether and water, separated, and the organic layer was washed with water, dried (MgS0 4 ), filtered and evaporated in vacuo. The residue was purified by flash chromatography, using a mixture of dichloromethane and methanol (99:1) as eluant, followed by crystallisation from diisopropyl ether, to give the title compound as a colourless solid, (3.43 g, 80%). m.p. 128-130°C. Η nmr (CDC1 3 ) δ: 2.23 (m, 2H), 2.40 (s, 3H), 2.42 (m, 2H), 2.58 (m, 2H), 3.30 (m, 2H), 3.58 (m, 2H), 3.87 (s, 3H), 4.00-4.10 (m, 4H), 6.00 (br, s, IH), 7.02 (dd, IH), 7.21 (d, IH), 7.49 (d, IH). LRMS :m/z 477 (M+18) + Anal. Found: C, 49.92; H, 5.40; N, 2.90. C 19 H 24 BrN0 5 S requires C, 49.78; H, 5.28; N, 3.06%. Preparation 24 4-(4-Bromo-3-methylphenyl)- 1 -(methylsulphonyl)piperidine Me Tπethylsilane (47.2ml, 296mmol), followed by tπfluoromethanesulphonic acid (1 73ml, 19 7mmol) were added to a solution of the sulphonamide from preparation 20 (65. Og, 197mmol) m dichloromethane (300ml) and tnfluoroacetic acid (300ml), and the reaction stined at room temperature for an hour. Tic analysis showed starting matenal remammg, so additional tnethylsilane (75.2ml, 471 mmol) and tnfluoromethanesulphonic acid (0 86ml, 9.8mmol) were added and the reaction stured for a further 20 hours at room temperature The reaction was concentrated m vacuo, the residue poured mto saturated aqueous potassium carbonate solution, and the mixture extracted with dichloromethane (3x650ml). The combmed organic extracts were washed with bnne (500ml), dned (MgS0 4 ), filtered and concentrated m vacuo The crude product was tnturated with hot methanol/hexane, filtered and dned to give the title compound (52 43g, 80%) as a buff-coloured solid. Η nmr (CDC1 3 , 400MHz) δ 1.78 (m, 2H), 1.90 (m, 2H), 2.37 (s, 3H), 2.52 (m, IH), 2.77 (m, 5H), 3.94 (m, 2H), 6.83 (m, IH), 7.02 (s, IH), 7.42 (m, IH). LRMS : m/z 354, 356 (M+23) + Preparation 25 Methyl 2-[4-(4-bromo-3-methylphenyl)pιρendm-l-ylsulphonyl]acetate Sodium hydride (12.2g, 60% dispersion m mineral oil, 305mmol) was added to a solution of the sulphonamide from preparation 24 (50.61g, 152mmol) and dimethylcarbonate (63.8ml, 760mmol) in toluene (600ml), and die reaction heated under reflux for 1 l A hours. The reaction was partitioned between ethyl acetate (1000ml), and cooled hydrochloπc acid (600ml, IN), and the layers separated. The aqueous layer was extracted with ethyl acetate (500ml), the combined orgamc extracts washed with brine (3x300ml), dned (MgS0 4 ), filtered and concentrated in vacuo. The residue was tnturated with hexane, and the solid filtered. This was re-crystallised from di-isopropyl ether and dned m vacuo to give the title compound as buff-coloured crystals, (40.9g, 69%) Η nmr (CDC1 3 , 400MHz) δ: 1.77 (m, 2H), 1.84 (m, 2H), 2.37 (s, 3H), 2.58 (m, IH), 2.97 (m, 2H), 3.80 (s, 3H), 3.96 (m, 4H), 6.84 (m, IH), 7.02 (s, IH), 7.42 (d, IH). LRMS m/z 412, 414 (M+23) + Preparation 26 Methyl 2-[4-(4-bromo-3-methylphenyl)piperidin- 1 -ylsulphonyl]-2-methyl-propanoate Triethylsilane (1.43ml, 9.0mmol) followed by trifluoromethanesulphonic acid (0.02ml, 0.3mmol) were added to a solution of the 1,2,3,6-tetrahydropyridine from preparation 22 (1.25g, 3. Ommol) and trifluoroacetic acid (15ml) in dichloromethane (15ml), and the reaction was stined for an hour at room temperature. The reaction mixture was concentrated in vacuo, the residue diluted widi dichloromethane (25ml), then partitioned between ethyl acetate (150ml) and saturated sodium bicarbonate solution (150ml), and the layers separated. The aqueous phase was extracted with ethyl acetate (2x35ml), the combined organic solutions dried (MgS0 4 ), filtered and evaporated in vacuo. The residual solid was triturated with di-isopropyl ether to give the title compound as a white solid, (963mg, 77%). mp 103-106°C 'H nmr (DMSO-c- 400MHz) δ: 1.52 (m, 8H), 1.77 (m, 2H), 2.28 (s, 3H), 2.63 (m, IH), 3.00 (m, 2H), 3.70 (m, 5H), 6.98 (dd, IH), 7.20 (s, IH), 7.42 (dd, IH). Anal. Found: C, 48.42; H, 5.74; N, 3.27. C 17 H 24 BrNS0 4 requires C, 48.81; H, 5.78 N, 3.35%. Preparation 27 Methyl 4-[4-(4-bromo-3-methylρhenyl)piperidin- 1 -ylsulphonyl]tetiahydro-2H-pyran-4-carboxylate Sodium hydride (60% dispersion in mineral oil, 1.16g, 29.0mmol) was added to a stined solution of the acetate from preparation 25 (10.14 g, 26.0mmol) in N-methyl pynolidinone (60 ml) at ambient temperature under nitrogen After 45 minutes, bιs-2-bromoethyl ether (4 26 ml, 33 8 mmol) was added to the stined mixture, and after a further 150 minutes an additional portion of sodium hydπde (60% dispersion in mineral oil, 1 16 g, 29 mmol) was added, and the mixture left stirnng for 18 hours The solvent was removed under reduced pressure, and the residues was partitioned between ethyl acetate and water The organic layer was collected, washed with bnne, dπed (MgS0 4 ), and evaporated under reduced pressure The residue was crystallised from ethyl acetate and diisopropyl ether to give the title compound as a colourless solid (7 34 g, 61%) The filtrate was evaporated and purified by flash chromatography eluting with dichloromethane, and crystallisation from ethyl acetate and diisopropyl ether to give an additional batch of the title compound as a colourless solid (1 86 g, 15%) A small sample was recrystallised from ethyl acetate for further charactensation m p 162-163°C Ηnmr (CDC1 3 ) δ 1 65-1 83 (m, 4H), 2 20 (m, 2H), 2 38 (s, 3H), 2 40 (m, 2H), 2 57 (m, IH), 3 00 (m, 2H), 3 29 (m, 2H), 3 85 (s, 3H), 3 87-4 00 (m, 4H), 6 83 (d, IH), 7 02 (s, IH), 7 41 (d, IH) LRMS m/z 460/462 (M+l) + Anal Found C,49 49, H,5 68, N,2 93 C 19 H 26 BrN0 5 S requires C,49 57, H,5 69, N,3 04% Alternative Route Triethylsdane (50ml, 0 30mol) was added dropwise over 2 mm to a solution of the carbmol from preparation 130 (60g, 0 12mol) m dichloromethane (150ml) and tnfluoroacetic acid (150ml), at 0°C, under nitrogen Tnflic acid (0 53ml, 6 Ommol) was added dropwise over 10 mm and the resulting mixture was stined at 0°C for 4h Dichloromethane (300ml) and demineralised water (300ml) were added and the aqueous phase was separated The organic phase was washed with water (200ml), saturated sodium bicarbonate solution (2x200ml) and demineralised water (200ml) and then concentrated in vacuo to a colourless solid The solid was slurned in hot ethyl acetate (300ml) for 20 mm and the mixture was cooled to 0°C and then filtered The residue was dπed in vacuo to leave the title compound as a colourless solid (53g, 92%) Preparation 28 Methyl l-benzyl-4-[4-(4-bromo-3-methylphenyl)ριpendm-l-ylsulphonyl]-4-pιpendmecarboxylate The acetate from preparation 25 (4 17g, 10 7mmol) was added portionwise to a suspension of sodium hydnde (994mg, 60% dispersion in mineral oil, 33 lmmol) in l-methyl-2-pyrrolιdmone (40ml), and the resultmg solution stined for an hour Tetra-butyl ammonium bromide (3 44g, 10 7mmol) and N-benzyl- bιs-(2-chloroethyl)amme (2.73g, 10 lmmol) were added portionwise, and once addition was complete, the reaction was stured at 60°C for 6 hours The cooled reaction was partitioned between water and ethyl acetate, the layers separated, and the aqueous phase exfracted with ethyl acetate The combmed organic extracts were washed with water, dried (Na 2 S0 4 ), filtered and concentrated m vacuo The crude product was punfied by column chromatography on silica gel twice, usmg an elution gradient of dιchloromethane:ether (100:0 to 90 10) to afford the title compound (3.04g, 52%) 'H nmr (CDC1 3 , 400MHz) δ: 1.63-1 81 (m, 4H), 1.88 (m, 2H), 2 16 (m, 2H), 2.36 (s, 3H), 2 42 (m, 2H), 2.55 (m, IH), 2.88 (m, 2H), 2.98 (m, 2H), 3.40 (s, 2H), 3.82 (m, 5H), 6.83 (d, IH), 7.00 (s, IH), 7.22 (m, 5H), 7.40 (d, IH). LRMS m z 549, 551 (M+l) + Preparation 29 Methyl 2-methyl-2-{4-[tπfluoromethanesulphonyloxy]-l,2,3,6-tetrahydropyndm-l- ylsulphonyljpropanoate 2,6-Dι-tert-butyl-4-methylpyπdme (3.7g, lδmmol) was added to a solution of the ketone from preparation 17 (3.8g, 14.5mmol) m dichloromethane (50ml), and the solution then cooled to 4°C. Trifluoromethane sulphonic anhydride (2.95ml, 17.5mmol) was added dropwise, and the reaction then stirred at room temperature for 17 hours Tic analysis showed startmg matenal remammg, so additional 2,6-dι-tert-butyl-4-methylpyndme (3.7g, 18mmol) and tnfluoromethane sulphonic anhydnde (2 7ml, 16mmol) were added portionwise to the stined reaction over the followmg 4 days. The mixture was then filtered, the filtrate concentrated m vacuo, and the residue tnturated with ether. The resultmg solid was filtered off, and the filtrate evaporated in vacuo This crude product was punfied by column chromatography on silica gel using an elution gradient of hexane ethyl acetate (91 9 to 50 50) to afford the title compound (4 25g, 74%) as a white solid Η nmr (CDC1 3 , 400MHz) δ 1 64 (s, 6H), 2 56 (m, 2H), 3 60 (m, 2H), 3 79 (s, 3H), 4 06 (m, 2H), 5.80 (m, IH) Anal Found C, 33 62, H, 4 03, N, 3 43 C π H, 6 F 3 N0 7 S 2 requires C, 33 42, H, 4.08, N, 3.54% Preparation 30 Methyl 2-[4-(4-{3-formylphenyl}-3-methylphenyl)-pιpeπdιn-l-ylsulphonyl]tetrahydro-2H-pyran-4- carboxylate A mixture of the bromide from preparation 27 (4 02g, 8.73mmol), 3-formylphenylboronιc acid (1.83g, 11.56mmol), cesium fluonde (3.46g, 22 8mmol), tns(dιbenzyhdeneacetone)palladιum (0) (430mg, 0.47mmol) and tn(o-tolyl)phosphιne (284mg, 0.93mmol) m 1,2-dιmethoxyethane (70ml) was heated under reflux for 6 hours. The cooled reaction was diluted with water and the mixture extracted with ethyl acetate (3x) The combmed organic extracts were washed with bnne, dπed (MgS0 4 ), filtered and concentrated under reduced pressure. The residue was punfied by column chromatography on silica gel usmg an elution gradient of ethyl acetate:hexane (25:75 to 40 60), and tnturated with di-isopropyl ether to give the title compound as a solid, (2.69g, 63%). *H nmr (CDC1 3 , 400MHz) δ: 1.75-1.95 (m, 4H), 2.20 (m, 5H), 2.40 (m, 2H), 2.62 (m, IH), 3.03 (m, 2H), 3.30 (m, 2H), 3.82-4.02 (m, 7H), 7.07 (m, 2H), 7 16 (m, IH), 7.56 (m, 2H), 7.81 (m, 2H), 10.02 (s, IH). LRMS : m z 508 (M+23) + Preparation 31 Methyl 2-[4-(4-{6-[2-benzyloxy]ethoxypyndιn-2-yl}-3-methylphenyl)-l,2,3,6-tetrahydropyπdm-l- ylsulphonyl]-2-methyl-propanoate A mixture of the stannane from preparation 4 (2.8g, 5.4mmol) and the bromide from preparation 22 (1.5g, 3.62mmol), and tetrakis(triphenylphosphine)palladium (0) (205mg, O.l δmmol) in toluene (35ml) was heated under reflux overnight. The cooled mixture was evaporated in vacuo and the residue purified by column chromatography on silica gel using pentane:ethyl acetate (75:25) as eluant, to afford the title compound as a colourless oil, (1.7g, 83%). "H nmr (CDC1 3 , 300MHz) δ: 1.69 (s, 6H), 2.42 (s, 3H), 2.64 (m, 2H), 3.62 (t, 2H), 3.82 (m, 5H), 4.14 (m, 2H), 4.56 (t, 2H), 4.62 (s, 2H), 6.06 (s, IH), 6.77 (d, IH), 7.0 (d, IH), 7.22-7.42 (m, 8H), 7.62 (m, IH). LRMS : m/z 565 (M+l) + Preparation 32 Methyl 4-[4-(4-{6-[2-benzyloxy]ethoxypyridin-2-yl}-3-methylphenyI)-l,2,3,6-tetiahydropyridin-l- ylsulphonyl]tetrahydro-2H-pyran-4-carboxylate A mixture of the stannane from preparation 4 (1.74g, 3.36mmol) and the bromide from preparation 23 (l.lg, 2.4mmol) and tetrakis(triphenylρhosphine)palladium (0) (138mg, 0.14mmol) in toluene (16ml) was heated under reflux for 4 hours. The cooled reaction was diluted with water, and the mixture extracted with ether (3x). The combined organic extracts were washed with brine, dried (MgS0 4 ), filtered through Arbocel® and evaporated in vacuo. The residual yellow oil was purified by column chromatography on silica gel using an elution gradient of pentane:ether (50:50 to 25:75) to afford the title compound as a pale yellow oil, (1.18g, 81%). 'H nmr (CDC1 3 , 400MHz) δ: 2.22 (m, 2H), 2.42 (m, 5H), 2.62 (m, 2H), 3.34 (m, 2H), 3.60 (m, 2H), 3.82 (t, 2H), 3.88 (s, 3H), 4.01 (m, 2H), 4.09 (m, 2H), 4.55 (t, 2H), 4.61 (s, 2H), 6.05 (m, IH), 6.76 (d, IH), 6.99 (d, IH), 7.21-7.41 (m, 78H), 7.61 (m, IH). LRMS m/z 607 (M+l) + Preparation 33 Methyl l-benzyl-4-{[4-(4-{6-[2-benzyloxyethoxy]pyndιn-2-yl}-3-methylphenyl)pιpeπdιn-l- yl]sulphonyl}-pιpeπdm-4-carboxylate The stannane from preparation 4 (4.05g, 7.8mmol), followed by tns(tπphenylphosphme) palladium (0) (410mg, 0.35mmol) were added to a solution of the bromide from preparation 28 (3.91g, 7. lmmol) m toluene (50ml), and die reaction de-gassed, then heated under a nitrogen atmosphere reflux for 7 hours. Aqueous potassium fluoπde solution (20ml, 25%) was added to the cooled reaction, the mixture stined at room temperature for 20 mmutes, then filtered through Arbocel®. The filtrate was diluted with ethyl acetate, washed with bnne, dned (Na 2 S0 4 ), filtered and evaporated m vacuo. The residue was punfied by column chromatography on silica gel twice, usmg an elution gradient of ethyl acetate:hexane (40:60 to 60:40) to give the desued product as a yellow crystalline solid, (2.77g, 56%) 'H nmr (CDC1 3 , 400MHz) δ: 1.74-1.95 (m, 6H), 2.17 (m, 2H), 2.37 (s, 3H), 2.44 (m, 2H), 2.60 (m, IH), 2.88 (m, 2H), 3.00 (m, 2H), 3.40 (s, 2H), 3 80 (m, 5H), 3 88 (m, 2H), 4.52 (t, 2H), 4.59 (s, 2H), 6J0 (d, IH), 6.95 (d, IH), 7.03 (m, 2H), 7.18-7.37 (m, 1 IH), 7.58 (m, IH). LRMS : m/z 699 (M+l) + Preparation 34 Methyl 2-[4-(4- {3-[2,2-dιethoxyethoxy]ρhenyl} -3-methylphenyl)- 1 ,2,3,6-tetrahydropyndm- 1 - ylsulphonyl]-2-methyl-propanoate A mixture of cesium fluoride (1.81g, 11.92mmol), tri-o-tolyl phosphine (180mg, 0.59mmol), tris(dibenzylideneacetone)dipalladium (0) (280mg, 0.3 lmmol) and the boronic acid from preparation 10 (1.83g, 7.2mmol) and the bromide from preparation 22 (2.5g, 6. Ommol) in anhydrous 1,2- dimethoxyethane (60ml), was heated under reflux for 5 X A h. The cooled reaction mixture was partitioned between water and ethyl acetate, and this mixture filtered through Arbocel®. The filtrate was separated, the organic phase washed with water, then brine, dried (Na 2 S0 4 ), filtered and evaporated in vacuo. The residual green oil was purified by medium pressure column chromatography on silica gel using an elution gradient of pentane:ethyl acetate (100:0 to 85:15) to afford the title compound, (3.04g, 93%). Η nmr (CDC1 3 , 300MHz) δ: 1.24 (t, 6H), 1.69 (s, 6H), 2.28 (s, 3H), 2.64 (m, 2H), 3.62 (m, 4H), 3.80 (m, 5H), 4.04 (d, 2H), 4.12 (m, 2H), 4.84 (t, IH), 6.06 (m, IH), 6.92 (m, 3H), 7.14-7.38 (m, 4H). LRMS : m z 563 (M+18) + Preparation 35 Methyl 2-[(4-{4-[6-(2-hydroxyethoxy)pyridin-2-yl]-3-methylphenyl}-piperidin-l-yl)sulphonyl]-2- methyl-propanoate A mixture of the benzyl ether from preparation 31 (1.7g, 3.0mmol), ammonium formate (3.0g, 50.0mmol), palladium hydroxide on carbon (500mg) and acetic acid (10ml) in methanol (30ml) was heated under reflux overnight. Additional ammonium formate (1.5g, 25.0mmol) and palladium hydroxide on carbon (1.5g) were added and the reaction heated under reflux for a further 72 hours. The cooled mixture was filtered through Arbocel®, and the filter pad washed well with ethyl acetate. The combined filtrates were neutralised using saturated sodium bicarbonate solution, the phases separated, and the aqueous layer extracted with ethyl acetate (2x100ml). The combined organic extracts were dried (MgS0 4 ), filtered and evaporated in vacuo to give the title compound as a colourless solid, (1.2g, 84%). mp 108-11 1°C Η nmr (CDC1 3 , 300MHz) δ: 1.64 (s, 6H), 1.78-1.94 (m, 4H), 2.40 (s, 3H), 2.65 (m, IH), 3.07 (m, 2H), 3.82 (s, 3H), 3.97 (m, 411), 4.50 (t, 2H), 6.7 (d, IH), 7.00 (d, IH), 7.10 (m, 2H), 7.38 (d, IH), 7.65 (m, IH). LRMS : m/z 477 (M+l) + Preparation 36 Methyl 4- { [4-(4- {6-[2-hydroxyethoxy]pyridin-2-yl} -3-methylphenyl)piperidin- 1 - yl] sulphonyl } tetrahydro-2H-pyran-4-carboxylate The title compound was prepared from the benzyl ether from preparation 32 in 93% yield, following a similar procedure to that described in preparation 35. Η nmr (CDC1 3 , 300MHz) δ: 1J0-1.95 (m, 4H), 2.22 (m, 2H), 2.40 (m, 5H), 2.64 (m, IH), 3.06 (m, 2H), 3.34 (m, 2H), 3.92 (m, 7H), 4.00 (m, 2H), 4.50 (t, 2H), 6.78 (d, IH), 7.00 (d, IH), 7.10 (m, 2H), 7.38 (d, IH), 7.65 (m, IH). LRMS : m/z 519 (M+l) + Preparation 37 Methyl 4-({4-[4-(6-{[(4R)-2,2-dimethyl-l,3-dioxolan-4-yl]methoxy}pyridin-2-yl)-3- methylphenyljpiperidin- 1 -yl} sulphonyl)tetrahydro-2H-pyran-4-carboxylate A mixture of the stannane from preparation 5 (2.0g, 4.97mmol) and the bromide from preparation 27 (1.76g, 3.82mmol) and tetrakis(triphenylphosphine)palladium (0) (242mg, 0.21mmol) in toluene (50ml) was heated under reflux for 7 hours. The cooled mixture was concentrated under reduced pressure and the residue purified by column chromatography on silica gel twice, using an elution gradient of etheπpentane (66:34 to 34:66) to give the title compound as a white solid, (1.29g, 57%). Η nmr (CDC1 3 , 300MHz) δ: 1.40 (s, 3H), 1.46 (s, 3H), 1.77-1.95 (m, 4H), 2.21 (m, 2H), 2.40 (m, 5H), 2.64 (m, IH), 3.04 (m, 2H), 3.34 (m, 2H), 3.81-4.04 (m, 8H), 4.15 (dd, IH), 4.40 (m, 2H), 4.50 (m, IH), 6.75 (d, IH), 7.00 (d, IH), 7.09 (m, 2H), 7.38 (d, IH), 7.62 (m, IH). LRMS : m/z 61 1 (M+23) + Preparation 38 Methyl 4-({4-[4-(6-{[(4S)-2,2-dimethyl-l,3-dioxolan-4-yl]methoxy}pyridin-2-yl)-3- methylphenyljpiperidin- 1 -yl} sulphonyl)tetiahydro-2H-pyran-4-carboxylate The title compound was obtained as a white solid (65%), after recrystallisation from methanol, from the stannane from preparation 6 and the bromide from preparation 27, following a similar procedure to that described in preparation 37. Η nmr (CDC1 3 , 300MHz) δ: 1.40 (s, 3H), 1.46 (s, 3H), 1.78-1.95 (m, 4H), 2.21 (m, 2H), 2.42 (m, 5H), 2.65 (m, IH), 3.08 (m, 2H), 3.35 (m, 2H), 3.81-4.05 (m, 8H), 4.14 (dd, IH), 4.40 (m, 2H), 4.50 (m, IH), 6.76 (d, IH), 6.99 (d, IH), 7.08 (m, 2H), 7.38 (d, IH), 7.62 (m, IH). LRMS : m/z 589 (M+l) + Preparation 39 Methyl 4- {[4-(4- {6-[(2S)-2,3-dihydroxy- 1 -propoxy]pyridin-2-yl} -3-methylρhenyl)piperidin- 1 - yl]sulphonyl}tetrahydro-2H-pyran-4-carboxylate A solution of the dioxolane from preparation 37 (799mg, 1 36mmol) m 1,4-dioxan (10ml) was added to an ice-cooled solution of hydrochloπc acid (30ml, 2N), and the reaction stined for 75 mmutes The solution was poured into saturated sodium bicarbonate solution (200ml), and the resultmg precipitate filtered and dned The solid was recrystallised from ethy acetate/di-isopropyl ether, to afford the desired product as a white powder, (642mg, 86%) Η nmr (CDC1 3 , 300MHz) δ. 1.70-2 42 ( , 12H), 2 64 (m, IH), 3 04 (m, 2H), 3 34 (m, 2H), 3 63 (m, 6H), 3 84-4 19 (m, 5H), 4.50 (m, 2H), 6.77 (d, IH), 7.00 (d, IH), 7 09 (m, 2H), 7.35 (d, IH), 7.68 (m, IH) Preparation 40 Methyl 4- { [4-(-4- {6-[(2R)-2,3-dιhydroxy- 1 -propoxy]pyndιn-2-yl} -3-methylphenyl)pιpendιn- 1 - yl]sulphonyl}tetrahydro-2H-pyran-4-carboxylate The title compound was obtamed as a white crystallme solid (86%), from the dioxolane from preparation 38, followmg the procedure descπbed m preparation 39. 'H nmr (CDC1 3 , 400MHz) δ: 1.76-1.92 (m, 4H), 2.21 (m, 2H), 2.40 (m, 5H), 2.50 (t, IH), 2.64 (m, IH), 3.06 (m, 2H), 3.34 (m, 2H), 3.64 (m, 2H), 3.72 (m, 5H), 4.00 (m, 3H), 4.12 (d, IH), 4 50 (m, 2H), 6.78 (d, IH), 7.01 (d, IH), 7.10 (m, 2H), 7.36 (d, IH), 7.68 (m, IH). LRMS : m/z 571 (M+23) + Preparation 41 Methyl 4- { [4-(4- {6-[2-hydroxyethoxy]pyndιn-2-yl} -3-methylphenyl)pιpeπdιn- 1 -yljsulphonyl} - pιpeπdιne-4-carboxylate A mixture of the benzyl pipeπdine from preparation 33 (3.32g, 4 76mmol), ammonium formate (3.0g, 47 6mmol) and palladium hydroxide on carbon (3.32g) m a solution of acetic acid methanol tetrahydrofuran (2.2.1, 30ml) was heated under reflux for 2 hours. The cooled reaction was filtered through Arbocel®, washmg through with tetrahydrofuran, and the filtrate concentrated m vacuo The residue was partitoned between water and ethyl acetate, and the layers separated The organic phase was dned (Na 2 S0 4 ), filtered and evaporated m vacuo. The crude product was punfied by column chromatography on silica gel usmg an elution gradient of dιchloromethane:methanol (90.10 to 85: 15) to afford the title compound, ( 1.28g, 52%). 'H nmr (CDC1 3 , 400MHz) δ: 1.73-1.88 (m, 4H), 2.00 (m, 2H), 2.38 (s, 3H), 2.42-2.64 (m, 5H), 3.02 (m, 2H), 3.16 (m, 2H), 3 85 (m, 7H), 4.46 (t, 2H), 6.73 (d, IH), 6 98 (d, IH), 7.05 (m, 2H), 7.34 (d, IH), 7.60 ( , IH). LRMS : m/z 518 (M+l) + Preparation 42 Methyl 4- {[4-(4- {6-[2-hydroxyethoxy]pyndιn-2-yl} -3-methylphenyl)pιpeπdιn- l-yl]sulphonyl} - 1 - methylpιpendιne-4-carboxylate Formaldehyde (0 49ml, 37 wt % in water, 4.9mmol) was added to a solution of the pipendine from preparation 41 (634mg, 1 22mmol) in dichloromethane (30ml), and the solution was stured vigorously at room temperature for 30 minutes Sodium tnacetoxyborohydnde (519mg, 2.45mmol) was added and the reaction was stined at room temperature for 20 hours The reaction was washed with water, dned (Na 2 S0 4 ), filtered and evaporated in vacuo The crude product was puπfied by column chromatography on silica gel using dichloromethane methanol (95 5) as eluant to give the title compound (559mg, 86%) Η nmr (CDC1 3 , 400MHz) δ. 1.76-1.95 (m, 6H), 2 20 (m, 5H), 2.38 (s, 3H), 2 50 (m, 2H), 2 62 (m, IH), 2 90 (m, 2H), 3.03 (m, 2H), 3.84 (s, 3H), 3 94 (m, 4H), 4 48 (m, 2H), 6 76 (d, IH), 6 99 (d, IH), 7 06 (m, 2H), 7.35 (d, IH), 7 63 (m, IH) LRMS : m/z 554 (M+23) + Preparation 43 Methyl l-(tert-butoxycarbonyl)- 4-{[4-(4-{6-[2-hydroxyethoxy]pyπdm-2-yl}-3-methylphenyl)pιpeπdm- l-yl]sulphonyl}-4-pιpeπdmecarboxy!ate Tπethylamine (175μl, 1.26mmol) was added to a solution of the amme from preparation 41 (594mg, 1.15mmol) m dichloromethane ( 100ml), followed by portionwise addition of di-tert-butyl dicarbonate (262mg, 1.20mmol). The reaction mixture was stined at room temperature for an hour, then concentrated in vacuo to a volume of 20ml. The solution was diluted with ether (150ml), washed with hydrochloπc acid (0.5N), bnne, then dπed (MgS0 4 ), filtered and evaporated m vacuo. The residue was puπfied by column chromatography on silica gel usmg dιchloromethane:methanol (95:5) as eluant to give the title compound (653mg, 92%) as a white foam 'H nmr (CDC1 3 , 400MHz) δ: 1.42 (s, 9H), 1.75-1.90 (m, 4H), 2.01 (m, 2H), 2.38 (s, 3H), 2.45 (m, 2H), 2.63 (m, 3H), 3.02 (m, 2H), 3.50 (m, IH), 3.87 (m, 7H), 4.17 (m, 2H), 4.46 (m, 2H), 6 75 (m, IH), 6.98 (m, IH), 7.05 (m, 2H), 7.35 (m, IH), 7.62 (m, IH). LRMS : m z 640 (M+23) + Preparation 44 Methyl 2-[4-(4-{3-tert-butoxyphenyl}-3-methylρhenyl)-pιpendιn-l-ylsulphonyl]acetate Nitrogen was bubbled through a mixture of cesium fluoride (3.71g, 24.44mmol), tri-o-tolyl phosphine (34mg, 0.1 lmmol), fris(dibenzylideneacetone)dipalladium (0) (50mg, 0.05mmol) the bromide from preparation 25 (4.27g, 11. Ommol) and the boronic acid from preparation 8 (3.2g, 16.5mmol) in anhydrous 1,2-dimethoxyethane (40ml). The reaction was then heated at 90°C under a nitrogen atmosphere for 50 hours. The cooled reaction mixture was diluted with ethyl acetate, the mixture washed with water (3x), dried (MgS0 4 ), filtered and concentrated in vacuo. The residue was purified by column chromatography on silica gel using an elution gradient of hexane:ethyl acetate (95:5 to 50:50) to give the title compound as an oil, that crystallised on standing, (3.15g, 62%). Η nmr (CDC1 3 , 400MHz) δ: 1.36 (s, 9H), 1.83 (m, 2H), 1.97 (m, 2H), 2.22 (s, 3H), 2.62 (m, IH), 2.98 (m, 2H), 3.80 (s, 3H), 3.98 (m, 4H), 6.94 (m, 3H), 7.04 (m, 2H), 7.17 (d, IH), 7.23 (m, IH). LRMS : m/z 582 (M+23) + Preparation 45 Methyl 2-[4-(4- {3-tert-butoxyphenyl} -3-methylphenyl)-piperidin- l-ylsulphonyl]-2-methyl-propanoate Potassium tert-butoxide (13.63ml, 1M in tetiahydrofuran, 13.63mmol) was added dropwise to a solution of the acetate from preparation 44 (2.5g, 5.45mmol) and methyl iodide (3.4ml, 54.5mmol) in tetrahydrofuran, and once addition was complete, the reaction was stined at room temperature for 72 hours. The mixture was partitioned between ethyl acetate and water and the layers separated. The organic phase was dried (MgS0 4 ), filtered and evaporated in vacuo, to give the crude title compound, which was used without further purification (3.1g). Η nmr (CDC1 3 , 400MHz) δ: 1.36 (s, 9H), 1.63 (s, 6H), 1.77-1.94 (m, 4H), 2.22 (s, 3H), 2.63 (m, IH), 3.05 (m, 2H), 3.80 (s, 3H), 3.95 (m, 2H), 6.90-7.10 (m, 5H), 7.18 (m, IH), 7.24 (m, IH). LRMS : m/z 488 (M+l) + Preparation 46 Methyl 4-[4-(4-{3-tert-butoxyphenyl}-3-methylphenyl)-piperidin-l-ylsulphonyl]-tetiahydro-2H-pyran-4- carboxylate Nitrogen was bubbled through a mixture of cesium fluoride (2.19g, 14.43mmol), tri-o-tolyl phosphine (20mg, 0.065mmol), tris(dibenzylideneacetone)dipalladium (0) (30mg, 0.032mmol) and the bromide from preparation 27 (2.9g, 6.5mmol) and the boronic acid from preparation 8 (1.78g, 9.75mmol) in anhydrous 1,2-dimethoxyethane (40ml). The reaction was then heated under reflux under a nitiogen atmosphere for 24 hours. The cooled reaction was partitioned between ethyl acetate and water, the organic phase dried (MgS0 4 ), filtered and concentrated in vacuo. The residue was triturated with diisopropyl ether, the solid filtered and dried under vacuum, to give the desired product as a cream- coloured solid, (2.0g, 58%). The filtrate was concentrated in vacuo and the residual oil purified by column chromatography on silica gel using an elution gradient of hexane: dichloromethane :methanol (50:50:0 to 0:100:0 to 0:99:1) to provide an additional (630mg, 18%) of the title compound. 'H nmr (CDC1 3 , 400MHz) δ: 1.37 (s, 9H), 1.76-1.92 (m, 4H), 2.20 (m, 5H), 2.40 (m, 2H), 2.60 (m, IH), 3.02 (m, 2H), 3.29 (m, 2H), 3.86 (m, 5H), 3.98 (m, 2H), 6.94 (m, 3H), 7.02 (m, 2H), 7.14 (m, IH), 7.22 (m, IH). LRMS : m z 552 (M+23) + Preparation 47 Methyl 2-[4-(4-{3-hydroxyphenyl}-3-methylρhenyl)-piperidin-l-ylsulρhonyl]-2-methyl-propanoate WO 00/74681 7S PCT/IBOO/00667 Trifluoroacetic acid (25ml) was added to a solution of the tert-butoxy ether from preparation 45 (4.8g, 9.80mmol) in dichloromethane (50ml), and the solution stined for 4 hours. The reaction mixture was concentrated in vacuo, and the residue purified by column chromatography on silica gel, twice using an elution gradient of dichloromethane :methanol (10:0 to 95:5) to give the desired product (536mg, 13%). Η nmr (CDC1 3 , 400MHz) δ: 1.62 (s, 6H), 1.76-1.92 (m, 4H), 2.22 (s, 3H), 2.62 (m, IH), 3.04 (m, 2H), 3.78 (s, 3H), 3.95 (m, 2H), 6.78 (m, 2H), 6.83 (m, IH), 7.03 (m, 2H), 7.15 (m, IH), 7.21 (m, IH). LRMS : m/z 454 (M+23) + Anal. Found: C, 63.70; H, 6.70; N, 3.20. C 23 H 29 N0 5 S requires C, 64.01; H, 6.77; N, 3.25%. Preparation 48 Methyl 4-[4-(4- {3-hydroxyphenyl} -3-methylphenyl)-piperidin- 1 -ylsulphonyl]-tetrahydro-2H-pyran-4- carboxylate Triethylsilane (2ml, 13.05mmol), followed by trifluoroacetic acid (5ml) were added to an ice-cooled solution of the tert-butyl ether from preparation 46 (2.3g, 4.35mmol) in dichloromethane (5ml) and the reaction stined for 2 hours. The mixture was concentrated in vacuo, and the residue azeotroped with toluene. The resulting foam was triturated with di-isopropyl ether, filtered and dried to afford the title compound as a solid, (1.94g, 94%). Alternative method Palladium (II) acetate (300mg, 1.34mmoI) and triphenylphosphine (708mg, 2.70mmol) were suspended in acetone (90ml), and sonicated for 2 minutes. The suspension was then added to a mixture of 5-bromo- 2-iodotoluene (7.9g, 27mmol), and the boronic acid from preparation 8 (5.7g, 29.4mmol) in aqueous sodium carbonate (42ml, 2N). The reaction mixture was heated under reflux for 2 hours, then cooled and diluted with water (300ml). This mixture was extracted with ether (2x250ml), the combined organic extracts dried (MgS0 4 ), filtered and evaporated in vacuo. The residue was purified by column chromatography on silica gel using hexane:ether (99: 1) as eluant to give 3-(4-bromo-2- methylphenyl)phenyl tert-butyl ether, 7.9g. A solution of this intermediate ether (480mg, 1.5mmol) in tetrahydrofuran (2ml), followed by a crystal of iodine, were added to magnesium (45mg, 1.8mmol), and the mixture was heated under reflux for 2 hours. The solution was diluted with tetrahydrofuran (3ml), cooled to -78°C, and a solution of the ketone from preparation 16 (425mg, 1.4mmol) in tetrahydrofuran (15ml) added dropwise. The reacton mixture was stined at -78°C for 30 minutes, then allowed to warm to room temperature. Aqueous ammonium chloride was added, the mixture extracted with ethyl acetate (2x50ml) and the combined organic extiacts were dπed (MgS0 4 ), filtered and evaporated in vacuo. The residue was purified by column chromatography on silica gel usmg pentane:ethyl acetate (50:50) to afford methyl 4-[4-(4-{3-tert-butoxyphenyl}-3- methylphenyl)-4-hydroxypιperidm-l-ylsulphonyl]-tetiahydro-2H-pyran-4-carboxylate as a clear oil, 280mg. Tπethylsilane (0.5ml, 3.14mmol), followed by tnfluoroacetic acid (5ml) were added to a solution of this intermediate (350mg, 0.64mmol) in dichloromethane (5ml), and the reaction stined at room temperature ovemight. The reaction mixture was concentrated m vacuo, the residue azeotroped with toluene and the resulting solid dπed under vacuum to afford the title compound, (300mg). Η nmr (CDC1 3 , 400MHz) δ: 1.74-1.90 (m, 4H), 2.20 (m, 5H), 2.40 (m, 2H), 2.62 (m, IH), 3.02 (m, 2H), 3.29 (m, 2H), 3.87 (m, 5H), 3.98 (m, 2H), 6.77 (m, 2H), 6.83 (d, IH), 7.02 (m, 2H), 7.15 (d, IH), 7.21 (m, lH). Preparation 49 Methyl 2-[4-(4-{3-[(2S)-2,3-dihydroxypropoxy]phenyl}-3-methylphenyl)-piperidin-l-ylsulphonyl]-2- methyl-propanoate A mixture of the alcohol from preparation 47 (800mg, 1.86mmol), S-glycidol (0.12ml, 1.86mmol), and triethylamine (lOμl, 0.09mmol) in methanol (10ml) was heated under reflux overnight. Tic analysis showed starting material remaining, so the mixture was concentrated to low volume, and heated under reflux for a further 4 hours. The cooled reaction was evaporated in vacuo and the residue purified by column chromatography on silica gel using an elution gradient of hexane: ethyl acetate (91:9 to 50:50). The desired product was obtained as an oil, that gave a white foam on drying under vacuum, (391mg, 42%). 'H nmr (DMSO-d^, 400MHz) δ: 1.50 (s, 6H), 1.58 (m, 2H), 1.80 (m, 2H), 2.18 (s, 3H), 2.67 (m, IH), 3.02 (m, 2H), 3.40 (m, 2H), 3.74 (m, 6H), 3.83 (m, IH), 3.98 (m, IH), 4.55 (m, IH), 4.80 (m, IH), 6.80 (m, 2H), 6.84 (m, IH), 7.05 (m, 3H), 7.26 (m, IH). LRMS: m/z 528 (M+23) + WO 00/74681 go PCT/IBOO/00667 Preparation 50 Methyl 4-[4-(4-{3-[l,3-dιbenzyloxy-2-propoxy]phenyl}-3-methylphenyl)-pιpeπdιn-l-ylsulphonyl]- tetrahydro-2H-pyran-4-carboxylate A mixture of the alcohol from preparation 48 (300mg, 0 63mmol), diethyl azodicarboxylate (150μl, 0 95mmol), tnphenylphosphine (250mg, 0 95mmol), and l,3-dιbenzyloxy-2-propanol (260mg, 0.95mmol) m tetrahydrofuran (6ml), was stured at room temperature for 3 hours Tic analysis showed some startmg matenal remammg, so additional 1 ,3-dιbenzyloxy-2-propanol (80mg, 0 3mmol), tπphenyl phosphme (80mg, 0.3mmol) and diethyl azodicarboxylate (50μl, 0.32mmol) were added, and stirring was continued for an hour. The mixture was evaporated m vacuo, and the residue punfied by column chromatography on silica gel usmg pentane-ethyl acetate (66.34) as eluant to give the title compound as a colourless oil, (400mg, 87%). 'H nmr (CDC1 3 , 400MHz) δ: 1.75-1.94 (m, 4H), 2.20 (m, 5H), 2.40 (m, 2H), 2.62 (m, IH), 3.04 (m, 2H), 3.30 (m, 2H), 3.75 (m, 4H), 3.89 (m, 5H), 3 99 (m, 2H), 4.57 (m, 5H), 6.89 (m, 3H), 7.02 (m, 2H), 7.14 (d, lH), 7.24 (m, l lH). Preparation 51 Methyl 4-[4-(4- {3-[ 1 ,3-dιhydroxy-2-propoxy]phenyl} -3-methylρhenyl)-pιpeπdm- 1 -ylsulphonyl]- tetrahydro-2H-pyran-4-carboxylate A mixture of the dibenzyl ether from preparation 50 (770mg, 1 Oόmmol), ammonium formate (1.4g, 11. Ommol) and palladium hydroxide on carbon (400mg) m methanol (40ml) was heated under reflux for 2 hours. Tic analysis showed some starting matenal remammg, so additional palladium hydroxide (300mg) was added, and the reaction was heated under reflux overnight The cooled mixture was filtered through Arbocel®, and the filtrate evaporated in vacuo The crude product was purified by column cliromatography on silica gel using ethyl acetate:pentane (84: 16) as eluant to afford the title compound as a white foam, (375mg, 65%). Η nmr (CDCI 3 , 400MHz) δ: 1.76-1.94 (m, 6H), 2.20 (m, 5H), 2.40 (m, 2H), 2.62 (m, IH), 3.04 (m, 2H), 3.29 (m, 2H), 3.90 (m, 10H), 3.99 (m, 2H), 6.94 (m, 3H), 7.03 (m, 2H), 7.16 (d, IH), 7.30 (m, IH). Preparation 52 Methyl 4-[4-(4-{3-[(2R)-2,3-dihydroxypropoxy]phenyl}-3-methylphenyl)-piperidin-l-ylsulphonyl]- tetrahydro-2H-pyran-4-carboxylate The title compound was obtained (17%) from the compound from preparation 48 and R-glycidol, following a similar procedure to that described in preparation 49. Η nmr (CDC1 3 , 400MHz) δ: 1.75-1.97 (m, 4H), 2.20 (m, 5H), 2.40 (m, 2H), 2.61 (m, IH), 3.02 (m, 2H), 3.28 (m, 2H), 3.58-4.14 (m, 12H), 6.84 (m, 3H), 7.02 (m, 2H), 7.15 (m, IH), 7.26 (m, IH). LRMS: m/z 570 (M+23) + Preparation 53 Methyl 4-[4-(4-{3-[(2S)-2,3-dihydroxypropoxy]phenyl}-3-methylphenyl)-piperidin-l-ylsulphonyl]- tetrahydro-2H-pyran-4-carboxylate The title compound was obtained as a white solid (52%) after recrystallisation from di-isopropylether, from the alcohol of preparation 48 and S-glycidol, following a similar procedure to that described in preparation 49. Η nmr (DMSO-d 6 , 300MHz) δ: 1.50-1.66 (m, 2H), 1.81 (m, 2H), 1.99 (m, 2H), 2.19-2.34 (m, 5H), 2.70 (m, IH), 3.06 (m, 2H), 3.20 (m, 2H), 3.43 (m, 2H), 3.70-3.98 (m, 9H), 4.00 (dd, IH), 4.60 (t, IH), 4.90 (d, IH), 6.80-6.95 (m, 3H), 7.15 (m, 3H), 7.31 (m, IH). LRMS: m/z 570 (M+23) + Preparation 54 Methyl 2-[4-(4-{3-(2,2-diethoxyethoxy)phenyl}-3-methylphenyl)-piperidin-l-ylsulphonyl]-2- methylpropanoate 20% Palladium hydroxide on carbon (250mg) was added to a solution of the 1,2,3,6-tetrahydropyridine from preparation 34 (3.0g, 5.5mmol) and ammonium formate (1.04g, 16.5mmol) in methanol (70ml) and 1,4-dioxan (28ml), and the reaction was stined at 60°C for 2 hours. Additional ammonium formate (1.0g, 15.8mmol) and palladium hydroxide on carbon (250mg) were added and stirring was continued for a further 2 hours. The mixture was cooled, filtered through Arbocel®, and the filter pad washed well with methanol. The combined filtrates were evaporated in vacuo and the residue partitioned between water and ether. The layers were separated, the organic phase washed with water, brine, dried (MgS0 4 ), filtered and evaporated in vacuo to give the title compound as a colourless oil, (2.8g, 93%). 'H nmr (CDC1 3 , 300MHz) δ: 1.22 (t, 6H), 1.68 (s, 6H), 1.78-1.96 (m, 4H), 2.25 (s, 3H), 2.64 (m, IH), 3.08 (m, 2H), 3.60-3.82 (m, 7H), 3.94-4.05 (m, 4H), 4.84 (t, IH), 6.90 (m, 3H), 7.09 (m, 2H), 7.18 (d, IH), 7.29 (d, IH). Anal. Found: C, 63.43; H, 7.75; N, 2.46. C^.NO.S requires C, 63.60; H, 7.55; N, 2.56%. Preparation 55 Metiιyl 4-[4-(4-{3-(2,2-diethoxyethoxy)phenyl}-3-methylphenyl)-piperidin-l-ylsulphonyl]-tetrahydro- 2H-pyran-4-carboxylate A mixture of cesium fluoride (4.3g, 28 3mmol), tn-o-tolyl phosphme (352mg, 1.15mmol), tns(dιbenzyhdeneacetone)dιpalladιum (0) (535mg, 0 59mmol) and the boronic acid from preparation 10 (3 89g, 14 95mmol) and bromide from preparation 27 (5 Og, 10 86mmol) m anhydrous 1,2- dimethoxyethane (70ml), was heated under reflux for 4 'A h The cooled reaction mixture was concentrated in vacuo to half its volume, then partitioned between water and ethyl acetate The layers were separated, the aqueous phase extracted with ethyl acetate (3x), and the combmed organic solutions filtered through Arbocel®. The filtrate was washed with bnne, dned (Na 2 S0 4 ), filtered and evaporated in vacuo The residual green oil was puπfied twice, by column chromatography on silica gel usmg an elution gradient of dιchloromethane:methanol (100.0 to 97:3), then tnturated with di-isopropyl ether, to afford the title compound as a white solid, (2.38g, 37%) Η nmr (CDC1„ 400MHz) δ- 1-20 (t, 6H), 1.76-1 94 (m, 4H), 2.20 (m, 5H), 2.40 (m, 2H), 2.61 (m, IH), 3.02 (m, 2H), 3.31 (m, 2H), 3.61 (m, 2H), 3 74 (m, 2H), 3.90 (m, 5H), 4.00 (m, 3H), 4.80 (m, IH), 6.85 (m, 3H), 7.03 (m, 2H), 7.16 (d, IH), 7.24 (m, 2H). LRMS: m/z 612 (M+23) + Preparation 56 Methyl 2-methyl-2-[4-(4-{3-(2-oxoethoxy)phenyl}-3-methylρhenyl)-pιperidm-l-ylsulphonyl]propanoate Hydrochloric acid (19ml, IN, 19mmol) was added to a solution of the diethyl ketal from preparation 54 (4.43g, 8. lmmol) m acetone (19ml) and 1,4-dioxan (22ml), and the reaction stined at 70°C for 2 hours. The cooled mixture was neutralised usmg sodium bicarbonate, concentrated in vacuo, and the residue partitioned between ether and water. The layers were separated, and the organic phase was washed with water, bnne, then dried (Na 2 S0 4 ), filtered and evaporated in vacuo The residue was azeotroped with ethyl acetate, to afford the title compound (quantitative) Η nmr (CDC1 3 , 300MHz) δ: 1.67 (s, 6H), 1.78- 1.96 (m, 4H), 2.26 (s, 3H), 2.66 (m, IH), 3.09 (m, 2H), 3.82 (s, 3H), 3.98 (m, 2H), 4.60 (s, 2H), 6.86 (m, 2H), 6.98 (d, IH), 7.09 (m, 2H), 7.17 (d, IH), 7.35 (m, IH), 9.90 (s, IH). LRMS : m/z 491 (M+18) + Preparation 57 Methyl 4-[4-(4-{3-(2-oxoethoxy)phenyl}-3-methylphenyl)-piperidin-l-ylsulphonyl]-tetiahydro-2H- pyran-4-carboxylate The title compound was obtained as a white foam (quantitative), from the diethyl ketal from preparation 55, following the procedure described in preparation 56. 'H nmr (CDC1 3 , 400MHz) δ: 1.77-1.93 (m, 4H), 2.21 (m, 5H), 2.40 (d, 2H), 2.62 (m, IH), 3.02 (m, 2H), 3.30 (m, 2H), 3.88 (m, 5H), 3.99 (m, 2H), 4.57 (s, 2H), 6.83 (m, 2H), 6.94 (d, IH), 7.03 (m, 2H), 7.15 (d, IH), 7.30 (m, IH), 9.83 (s, IH). Anal. Found: C, 61.79; H, 6.66; N, 2.46. C„H 33 NO 7 S;0.25CH 3 CO 2 C 2 H 5 ;0.4H 2 O requires C, 61.72; H, 6.62; N, 2.57%. Preparation 58 Methyl 2-memyl-2-[4-(4-{3-(2-me ylaminoethoxy)phenyl}-3-methylphenyl)-piperidin-l- ylsulphonyljpropanoate Sodium triacetoxyborohydride (1.5g, 7.08mmol) was added portionwise over 1 hour to a solution of the aldehyde from preparation 56 (l.Og, 2. lmmol) and methylamine (5.8ml, 2N in tetrahydrofuran, 1 l.όmmol) in dichloromethane (50ml), and once addition was complete, the reaction was stined at room temperature overnight. The reaction was partitioned between ethyl acetate and saturated sodium bicarbonate solution, and the layers separated, The organic phase was washed with water, brine, dried (Na 2 S0 4 ), filtered and evaporated in vacuo to give a colourless oil. This was purified by medium pressure column chromatography on silica gel using an elution gradient of dichloromethane:methanol (100:0 to 90:10) to afford the title compound as a foam, (650mg, 63%). Η nmr (CDC1 3 , 400MHz) δ: 1.62 (s, 6H), 1.76-1.90 (m, 4H), 2.22 (s, 3H), 2.56 (s, 3H), 2.61 (m, IH), 3.04 (m, 4H), 3.78 (s, 3H), 3.95 (m, 2H), 4.12 (t, 2H), 6.83 (m, 3H), 7.03 (m, 2H), 7.14 (d, IH), 7.24 (m, IH). Anal. Found: C, 58.39; H, 6.90; N, 4.97. C 26 H 36 N 2 O 5 S;0.75CH 2 Cl 2 requires C, 58.17; H, 6.84; N, 5.07%. Preparations 59 to 63 The compounds of the general formula: were prepared from the conesponding aldehydes and amines, following similar procedures to those described in preparation 58. WO 00/74681 PCT/IBO0/OO667 1 = purified by crystallisation from ethyl acetate/dichloromethane/di-isopropyl ether. 2 = purified by column chromatography on silica gel using ethyl acetate:pentane (75:25) as eluant, and recrystallised from ethyl acetate. Preparation 64 Methyl 2-[4-(4- {3-(2-[( -tert-butoxycarbonyl)(N-methyl)amino]ethoxy)phenyl} -3-methylphenyl)- piperidin- 1 -ylsulphonyl]-2-methyl-propanoate A mixture of the compound from preparation 58 (640mg, 1.3 lmmol), triethylamine (180μl, lJOmol), di- tert-butyl dicarbonate (290mg, 1.33mmol) and 4-dimethylaminopyridine (catalytic) in dichloromethane (10ml) was stirred at room temperature for 3 hours. The reaction mixture was diluted with dichloromethane (50ml), and washed with water, brine, dried (Na 2 S0 4 ), filtered and evaporated in vacuo. The residual oil was purified by medium pressure column chromatography on silica gel using an elution gradient of pentane dichloromethane methanol (100 0 0 to 0 99 5 0 5) to afford the title compound as a gum, (590mg, 77%) Η nmr (CDC1„ 400MHz) δ 1 42 (s, 9H), 1 62 (s, 6H), 1 77-1 90 (m, 4H), 2 22 (s, 3H), 2 63 (m, IH), 2 97 (s, 3H), 3 03 (m, 2H), 3 58 (m, 2H), 3 78 (s, 3H), 3 95 (m, 2H), 4 08 (m, 2H), 6 82 (m, 3H), 7 04 (m, 2H), 7 16 (d, IH), 7 25 (m, IH) LRMS m z 61 1 (M+23) + Anal Found C, 60 51, H, 7 19, N, 4 47 C 31 H 44 N 2 O 7 S,0 4CH 2 C1 2 requires C, 60 56, H, 7 25, N, 4 50% Preparation 65 Methyl 2-[4-(4-{3-(2-am oethoxy)phenyl}-3-methylphenyl)-pιpeπdιn-l-ylsulphonyl]-2-methyl- propanoate A mixture of the amme from preparation 60 (1 2g, 2 12mmol) and 20% palladium hydroxide on carbon (250mg) m methanol (75ml), was hydrogenated at 50psι and room temperature for 18 hours The reaction mixture was filtered through Arbocel®, and the filter pad washed well with methanol The combmed filtrates were evaporated m vacuo to give an oil This was punfied by medium pressure column chromatography on silica gel usmg an elution gradient of dichloromethane methanol (100 0 to 90 10) to afford the title compound (61 Omg, 60%) Η nmr (CDC1 3 , 300MHz) δ 1 66 (s, 6H), 1 78-1 97 (m, 4H), 2 28 (s, 3H), 2 66 (m, IH), 3 10 (m, 4H), 3 82 (s, 3H), 3 99 (m, 4H), 6 88 (m, 3H), 7 10 (m, 2H), 7 19 (d, IH), 7 30 (m, IH) LRMS m z 475 (M+l) + Anal Found C, 61 26, H, 7 09, N, 5 63 C 25 H 34 N 2 O 5 S,0 25dιchloromethane requires C, 61 16, H, 7 01, N, 5 65% Preparation 66 WO 00/74681 3g PCT/IBOO/00667 Methyl 4-[4-(4-{3-(2-aminoethoxy)phenyl}-3-methylρhenyl)-piperidin-l-ylsulphonyl]-tetrahydro-2H- pyran-4-carboxylate The title compound was obtained as a solid (65%) from the compound from preparation 61, following the procedure described in preparation 65. Η nmr (CDC1 3 , 400MHz) δ: 1.76-1.92 (m, 4H), 2.20 (m, 5H), 2.40 (m, 2H), 2.62 (m, IH), 3.04 (m, 4H), 3.30 (m, 2H), 3.88 (m, 5H), 3.98 (m, 4H), 6.82 (m, 3H), 7.03 (m, 2H), 7.16 (d, IH), 7.22 (m, IH). LRMS: m z 517 (M+l) + Anal. Found: C, 62.30; H, 6.98; N, 5.40. requires C, 62.37; H, 6.99; N, 5.38%. Preparation 67 Methyl 2-[4-(4-{3-(2-[(tert-butoxycarbonyl)amino]ethoxy)ρhenyl}-3-methylphenyl)-piperidin-l- ylsulρhonyl]-2-methyl-propanoate The title compound was obtained as a white foam (69%) from the amine from preparation 65, following a similar procedure to that described in preparation 64. Η nmr (CDC1 3 , 300MHz) δ: 1.44 (s, 9H), 1.65 (s, 6H), 1.78-1.95 (m, 4H), 2.25 (s, 3H), 2.64 (m, IH), 3.08 (m, 2H), 3.55 (m, 2H), 3.81 (s, 3H), 3.97 (m, 2H), 4.04 (t, 2H), 4.99 (br, s, IH), 6.80-6.94 (m, 3H), 7.08 (m, 2H), 7.18 (d, IH), 7.32 (m, IH). LRMS : m/z 597 (M+23) + Anal Found. C, 62 49, H, 7 46, N, 4 78 C 30 H 42 N 2 O 7 S requires C, 62 69, H, 7.37, N, 4 87% Preparation 68 Methyl 4-[4-(4-{3-(2-[(tert-butoxycarbonyl)ammo]ethoxy)phenyl}-3-methylphenyl)-pιpeπdιn-l- ylsulphonyl]-tetiahydro-2H-pyran-4-carboxylate Di-tert-butyl dicarbonate (300mg, 1.37mmol) was added to a solution of the amme from preparation 66 (650mg, 1.26mmol) in dichloromethane ( 10ml), and the reaction stirred at room temperature for 18 hours The reaction was diluted with dichloromethane (50ml), then washed with water (2x), bnne, then dned (Na 2 S0 4 ), filtered and evaporated m vacuo. The residue was punfied by medium pressure column chromatography on silica gel usmg an elution gradient of dιchloromethane:methanol (99.5:0.5 to 99:1) to afford the title compound as a white foam, (710mg, 91%). Η nmr (CDC1 3 , 400MHz) δ: 1.40 (s, 9H), 1.78-1.92 (m, 4H), 2.20 (m, 5H), 2.40 (d, 2H), 2.61 (m, IH), 3.02 (m, 2H), 3.30 (m, 2H), 3.50 (m, 2H), 3.88 (m, 5H), 4.00 (m, 4H), 4.86 (br s, IH), 6.82 (m, 3H), 7.02 (m, 2H), 7.15 (d, IH), 7.05 (m, IH). LRMS: m/z 639 (M+23) + Anal. Found: C, 62.15; H, 7.20, N, 4 47. C^NAS requires C, 62.32, H, 7.19; N, 4.54%. Preparation 69 Methyl 4-[4-(4-{3-([N-tert-butoxycarbonyl-N-methylamjno]methyl)phenyl}-3-methylphenyl)-pιpendm- l-ylsulphonyl]-teteahydro-2H-pyran-4-carboxylate The title compound was prepared from the amine from preparation 62, using a similar procedure to that descπbed in preparation 64 The crude product was purified by column chromatography on silica gel using an elution gradient of ethyl acetate pentane (25 75 to 50 50) and tnturated with di-isopropyl ether to give the title compound as a white solid, (714mg, 65%) mp 122-123°C Η nmr (CDC1 3 , 400MHz) δ 1 42 (s, 9H), 1 75-1 92 (m, 4H), 2 20 (m, 5H), 2 40 (m, 2H), 2 61 (m, IH), 2 82 (s, 3H), 3 03 (m, 2H), 3 30 (m, 2H), 3 85 (m, 5H), 3 99 (m, 211), 4 42 (s, 2H), 7 03 (m, 2H), 7 17 (m, 4H), 7 35 (m, IH) LRMS m/z 623 (M+23) + Anal Found C, 63 92, H, 7 36, N, 4 57 C^H^N^S requires C, 63 98, H, 7 38, N, 4 66% Preparation 70 2-[4-{4-[6-(2-Hydroxyethoxy)pyndm-2-yl]-3-methylphenyl}-pιpeπdm-l-ylsulphonyl]-2- methylpropanoic acid A mixture of the methyl ester from preparation 35 (4 lg, 8 6mmol) and aqueous sodium hydroxide (17ml, IN, 17 Ommol) in methanol (50ml), was heated under reflux for 30 mmutes, then cooled The reaction was concenfrated m vacuo, the residue dissolved m water (200ml), and the solution acidified to pH 4 The resultmg precipitate was filtered off, washed with water, dned under vacuum, and recrystallised from ethyl acetate, to afford the title compound as a white solid, (3 15g, 79%) 'H nmr (DMSO-d^, 300MHz) δ 1 42-1 70 (m, 8H), 1 80 (m, 2H), 2 37 (s, 3H), 2 70 (t, IH), 3 06 ( , 2H), 3 68 (m, 2H), 3 80 (m, 2H), 4 25 (t, 2H), 4 80 (br, s, IH), 6 77 (d, IH), 7 06 (d, IH), 7 17 (m, 2H), 7 35 (d, IH), 7 77 (m, IH), 13 38 (br, s, IH) Anal Found C, 58 35, H, 6 38, N, 5 83 C 23 H 30 N 2 O 6 S,0 5H 2 0 requires C, 58 85, H, 6 62, N, 5 94% Preparation 71 2-(4- {4-[6-(2-Methoxyethoxy)pyndm-2-yl]-3-methylphenyl} -pipeπdin- 1 -ylsulphonyl)-2- methylpropanoic acid Sodium hydride (60mg, 60% dispersion m mineral oil, 1.5mmol) was added to a solution of the methyl ester from preparation 35 (300mg, 0.63mmol) in tetrahydrofuran (10ml), and the solution stined for 15 minutes Methyl iodide (200μl, 3 3mmol) was added and the reaction heated under reflux for 45 mmutes. Aqueous sodium hydroxide solution (2ml, IN, 2 Ommol) and methanol (5ml) were then added, and the mixture heated under refux for a further 30 minutes. The reaction mixture was cooled to room temperature, diluted with water (20ml), and acidified to pH 4 This solution was extracted with dichloromethane (3x30ml), the combined organic extracts dπed (Na 2 S0 4 ), filtered and evaporated m vacuo to afford the title compound as a pale yellow foam, (quantitative) mp 142-146°C Η nmr (CDC1 3 , 300MHz) δ. 1.68 (s, 6H), 1.78-1.96 (m, 4H), 2 41 (s, 3H), 2.66 (m, IH), 3.09 (m, 2H), 3 43 (s, 3H), 3.78 (t, 2H), 4.00 (m, 2H), 4.52 (t, 2H), 6 78 (d, IH), 6 98 (d, IH), 7.08 (m, 2H), 7.38 (d, IH), 7 61 (d, IH). LRMS : m/z 433 (M-C0 2 ) + Preparation 72 4-[4-(4-{6-[2-Hydroxyethoxy]pyπdm-2-yl}-3-methylphenyl)pιpendm-l-ylsulphonyl]tetrahydro-2H- pyran-4-carboxyhc acid Aqueous sodium hydroxide (5.56ml, IN, 5 56mmol) was added to a solution of the methyl ester from preparation 36 (720mg, 1 39mmol) in methanol (20ml), and the reaction heated under reflux for 3 hours, and stined for a further 18 hours, at room temperature The mixture was concentiated in vacuo to remove the methanol, and the solution acidified to pH 4 using acetic acid solution This was extracted with ethyl acetate (3x), the combined organic extracts washed with brine, dried (MgS0 4 ), filtered and evaporated in vacuo The residual solid was recrystallised from ethyl acetate/di-isopropyl ether to afford the title compound as a solid, (517mg, 74%) Η nmr (DMSO-d 6 , 300MHz) δ 1 62 (m, 2H), 1.82 (m, 2H), 1.98 (m, 2H), 2 24 (m, 2H), 2 36 (s, 3H), 2 74 (m, IH), 3 09 (t, 2H), 3.22 (m, 2H), 3 64-3 82 (m, 4H), 3 94 (dd, 2H), 4.28 (t, 2H), 4 80 (br s, IH), 6 78 (d, IH), 7 06 (d, IH), 7.16 (m, 2H), 7 36 (d, IH), 7.78 (m, IH), 13.82 (br s, IH) LRMS - m/z 527 (M+18) + Preparation 73 4-[4-(4-{6-[(2S)-2,3-dιhydroxy-l-propoxy]pyndιn-2-yl}-3-methylphenyl)ριpeπdm-l-ylsulphonyl]- tetiahydro-2H-pyran-4-carboxylιc acιd Aqueous sodium hydroxide (3.5ml, 1M, 3.5mmol) was added to a solution of the methyl ester from preparation 39 (640mg, 1.17mmol) m methanol (15ml) and 1,4-dioxan (15ml), and the reaction heated under reflux for 2 hours. Tic analysis showed starting matenal remammg, so additonal sodium hydroxide (2ml, 1M, 2mmol) was added and the reaction heated under reflux for a further 3 hours The cooled reaction mixture was concenfrated under reduced pressure, the residue dissolved m water, and the pH adjusted to 4 usmg hydrochlonc acid (2N). The resultmg precipitate was filtered and dned, and the filtrate extracted with dichloromethane (2x). The combmed organic extracts were dπed (MgS0 4 ), filtered and evaporated in vacuo, and the product combmed with the filtered solid. This was recrystallised from dichloromethane/ethyl acetate twice, to yield the title compound as a white solid, (579mg, 92%). Η nmr (DMSO-d*, 400MHz) δ: 1.60 (m, 2H), 1.80 (m, 2H), 1.92 (m, 2H), 2.23 (d, 2H), 2.34 (s, 3H), 2.66 (m, IH), 3.08 (m, 2H), 3.17-3.42 (m, 3H), 3.78 (m, 3H), 3.88 (m, 2H), 4.14 (dd, IH), 4.26 (dd, IH), 4 60 (br, s, IH), 4.85 (br, s, IH), 6.76 (d, IH), 7.04 (d, IH), 7.15 (m, 2H), 7.34 (m, 2H), 7.74 (dd, IH). LRMS : m/z 557 (M+23) + Preparation 74 4-[4-(4- {6-[(2R)-2,3-dιhydroxy- l-propoxy]pyndιn-2-yl} -3-methylphenyl)pιpeπdιn- 1 -ylsulphonyl]- tetrahydro-2H-pyran-4-carboxyhc acid The title compound was obtained as a white solid (87%) from the methyl ester of preparation 40, following a similar procedure to that described in preparation 73. Η nmr (DMSO-d 6 , 300MHz) δ: 1.61 (m, 2H), 1.80 (m, 2H), 1.96 (m, 2H), 2.24 (m, 2H), 2.36 (s, 3H), 2.70 (m, IH), 3.06 (m, 2H), 3.14-3.44 (m, 4H), 3.78 (m, 3H), 3.93 (m, 2H), 4.14 (m, IH), 4.26 (m, IH), 4.59 (m, IH), 4.84 (m, IH), 6.76 (d, IH), 7.06 (d, IH), 7.15 (m, 2H), 7.35 (d, IH), 7.76 (m, IH), 13.80 (br, s, IH). LRMS : m/z 557 (M+23) + Preparation 75 4-[4-(4- {6-[2-Hydroxyethoxy]pyridin-2-yl} -3-methylphenyl)piperidin- 1 -ylsulphonyl]- 1 - methylpiperidine-4-carboxylic acid A mixture of the methyl ester from preparation 42 (200mg, O.38mmol) and aqueous sodium hydroxide (1.5ml, IN, 1.5mmol) in methanol (8ml) and 1,4-dioxan (8ml) was heated under reflux ovemight. The cooled reaction was concentrated in vacuo, the residue acidified to pH 4 using acetic acid, and extraction with ethyl acetate attempted. A precipitate formed in the organic layer, that was filtered off, and combined with the residual solid in the separating funnel, to provide the desired compound as a white powder, (quantitative). LRMS : m/z 518 (M+l) + Preparation 76 l-(tert-Butoxycarbonyl)- 4-[4-(4- {6-[2-hydroxyethoxy]pyτιdιn-2-yl}-3-methylphenyl)pιρeπdιn-l- ylsulphonyl]-pιpeπdιne-4-carboxylιc acid The title compound was obtained as a white solid (87%), from the methyl ester from preparation 43, following a similar procedure to that described in preparation 75. mp 148-149°C Η nmr (CDC1 3 , 300MHz) δ: 1.42 (s, 9H), 1.80 (m, 4H), 2.00 (m, 2H), 2.36 (s, 3H), 2.41 (m, 2H), 2.58- 2.79 (m, 4H), 3.02 (m, 4H), 3.92 (m, 5H), 4.44 (m, 2H), 6.76 (m, IH), 6.99 (m, IH), 7.07 (m, 2H), 7.34 (m, IH), 7.65 (m, IH). Preparation 77 2-[4-(4-{3-[(2S)-2,3-Dιhydroxy-l-propoxy]phenyl}-3-methylρhenyl)-piperidin-l-ylsulphonyl]-2- methyl-propanoic acid Aqueous sodium hydroxide (1.55ml, 1M, 1.55mmol) was added to a solution of the methyl ester from preparation 49 (391mg, 0.77mmol) in methanol (5ml), and the reaction stirred at room temperature ovemight. The mixture was partitioned between ethyl acetate and hydrochloric acid (2N), and the phases separated. The organic layer was dried (MgS0 4 ), filtered and concentrated in vacuo. The residual solid was tnturated with di-isopropyl ether, filtered and dried under vacuum, to give the title compound as a white solid, (320mg, 85%). 'H nmr (DMSO-d 6 , 400MHz) δ: 1.48 (s, 6H), 1.59 (m, 2H), 1.79 (m, 2H), 2.18 (s, 3H), 2.64 (m, IH), 3.04 (m, 2H), 3.40 (m, 2H), 3.78 (m, 3H), 3.82 (m, IH), 3.98 (m, IH), 4.57 (br, s, IH), 4.82 (br, s, IH), 6.80 (m, 2H), 6.85 (m, IH), 7.05 (m, 2H), 7.12 (m, IH), 7.27 (m, IH), 13.25 (br, s, IH). Anal Found C, 60 77, H, 6 89, N, 2 78 C 25 H 33 N0 7 S requires C, 61 08, H, 6 77, N, 2 85% Preparation 78 4-[4-(4- {3-[2,3-dιhydroxy-2-propoxy]phenyl} -3-methylphenyl)-pιpeπdιn- 1 -ylsulphonyl]-tetrahydro-2H- pyran-4-carboxyhc acid A mixture of the methyl ester from preparation 51 (370mg, 0 68mmol), aqueous sodium hydroxide (3ml, 1M, 3mmol) m methanol (5ml) and 1,4-dioxan (5ml), was heated under reflux for 6 hours The cooled reaction was concentrated m vacuo, and then diluted with water This aqueous solution was acidified to pH 2 usmg hydrochlonc acid (2N), and the resultmg precipitate filtered, washed with water and dned under vacuum, to give the desired product (270mg, 74%) Η nmr (DMSO-d^, 400MHz) δ 1.60 (m, 2H), 1.79 (m, 2H), 1.95 (m, 2H), 2.19 (m, 5H), 2 63 (m, IH), 3.02 (m, 4H), 3.56 (m, 4H), 3.76 (m, 2H), 3.88 (m, 2H), 4 22 (m, IH), 4.68 (m, 2H), 6.78-6 95 (m, 3H), 7.08 (m, 3H), 7.25 (m, IH). Preparation 79 4-[4-(4-{3-[(2R)-2,3-Dιhydroxy-l-propoxy]phenyl}-3-methylphenyl)-pιpendm-l-ylsulphonyl]- tetrahydro-(2H)-pyran-4-carboxylιc acid A mixture of the methyl ester from preparation 52 (1 lOmg, 0.20mmol), aqueous sodium hydroxide (1ml, 1M, lmmol) in methanol (5ml) and 1,4-dioxan (5ml) was heated under reflux for 2 hours The cooled reaction was evaporated in vacuo, the residue dissolved in water and acidified to pH 1 usmg hydrochlonc acid (IN) The resulting precipitate was filtered, the solid washed with water, and dπed under vacuum to give the title compound (91mg, 85%) as a white solid. Η nmr (DMSO-d 6 , 400MHz) δ: 1.60 (m, 2H), 1.80 (m, 2H), 1.94 (m, 2H), 2.20 (m, 5H), 2.65 (m, IH), 3.05 (m, 2H), 3.18-3.48 (m, 4H), 3.77 (m, 3H), 3.88 (m, 3H), 4.00 (m, IH), 6.81 (m, 2H), 6.89 (m, IH), 7.10 (m, 3H), 7.30 (m, IH). LRMS : m/z 556 (M+23) + Preparation 80 4-[4-(4- {3-[(2S)-2,3-Dihydroxy- 1 -propoxy]phenyl} -3-methylphenyl)-piperidin- 1 -ylsulphonyl]- tetrahydro-(2H)-pyran-4-carboxylic acid The title compound was obtained as a solid (66%) from the methyl ester from preparation 53, following the procedure described in preparation 79. Η nmr (DMSO-d,, 400MHz) δ: 1.60 (m, 2H), 1.80 (m, 2H), 1.96 (m, 2H), 2.22 (m, 5H), 2.68 (m, IH), 3.06 (m, 2H), 3.21 (m, 2H), 3.42 (d, 2H), 3.78 (m, 3H), 3.90 (m, 3H), 4.00 (m, IH), 6.81 (m, 2H), 6.90 (d, IH), 7.12 (m, 311), 7.31 (dd, IH). Preparation 81 2-[4-(4-{3-(2-[ -tert-Butoxycarbonyl-N-methylamino]ethoxy)phenyl}-3-methylphenyl)-piperidin-l- ylsulphonyl]-2-methylpropanoic acid A mixture of the methyl ester from preparation 64 (540mg, 0.92mmol), and aqueous sodium hydroxide (6ml, IN, 6.0mmol) in 1,4-dioxan (2.3ml) and methanol (6ml) was heated under reflux for 3 A h. The cooled mixture was concentiated in vacuo to remove the organic solvents, and the residual aqueous solution was acidified to pH 4 using acetic acid. This was extracted with ethyl acetate (2x), the combined organic extracts washed with water, brine, dried (Na 2 S0 4 ), filtered and evaporated in vacuo. The residue was azeotroped with toluene, then ethyl acetate, and finally dichloromethane to afford the title compound as a white foam, (520mg, 98%) 'H nmr (CDC1 3 , 400MHz) δ 1 41 (s, 9H), 1 64 (s, 6H), 1 78-1 94 (m, 4H), 2 22 (s, 3H), 2 63 (m, IH), 2 97 (s, 3H), 3 06 (m, 2H), 3 59 (m, 2H), 3 98 (m, 2H), 4 08 (t, 2H), 6 83 (m, 3H), 7 04 (m, 2H), 7 16 (d, IH), 7 26 (m, IH) LRMS m/z 597 (M+23) + Anal Found C, 61 17, H, 7 27, N, 4 65 C 30 H 42 N 2 O 7 S,0 2CH 2 C1 2 requires C, 61 30, H, 7 22, N, 4 73% Preparations 82 to 86 The compounds of the general formula were prepared from the coπesponding methyl esters, followmg similar procedures to those descπbed in preparation 81 1 = isolated by filtration from aqueous acetic acid solution. 2 = recrystallised from ethyl acetate/methanol 3 = tnturated with di-isopropyl ether Preparation 87 N-Hydroxy l-(tert-butoxycarbonyl)-4-{[4-(4-{6-[2-hydroxyethoxy]pyπdιn-2-yl}-3- methylphenyl)pιpeπdm- 1 -yl] sulphonyl } -pιpeπdιne-4-carboxamιde Chlorotrimethylsilane (70μl, 0.55mmol) was added to a solution of the acid from preparation 76 (300mg, 0.50mmol) m dichloromethane (4ml), and pyndine (2ml), and the solution stined at room temperature under a nitrogen atmosphere for 1 hour l-(3-Dιmethylamιnopropyl)-3-ethylcarbodιimιde hydrochloπde (1 15mg, O.όOmmol) and l-hydroxy-7-azabenzotπazole (75mg, 0.55mmol) were added, and stirnng was continued for a further hour Hydroxylamine hydrochloride (104mg, 1.50mmol) was added and the reaction stured at room temperature ovemight The reaction mixture was diluted with water, the solution acidified to pH 1 using hydrochloπc acid (2M), then extracted with ethyl acetate. The combined organic solutions were washed with brine, dried (MgS0 4 ), filtered and evaporated in vacuo. The residue was tnturated with ethyl acetate, the resulting precipitate filtered and the filtrate evaporated m vacuo. The residue was recrystallised from ethyl acetate to afford the title compound (148mg, 48%) as a white solid. mp 180-181°C Η nmr (DMSO-d 6 , 400MHz) δ: 1.39 (s, 9H), 1.55-1.81 (m, 6H), 2 36 (s, 3H), 2.42 (m, 2H), 2.62 (m, 3H), 3.03 (m, 2H), 3.70 (m, 4H), 3.95 (m, 2H), 4.24 (t, 2H), 4.78 (br, t, IH), 6.75 (d, IH), 7.04 (d, IH), 7.15 (m, 2H), 7.34 (d, IH), 7.75 (m, lH), 9.16 (s, IH), 11.00 (s, IH). LRMS : m z 617 (M-l) + Preparation 88 N-Hydroxy 2-[4-(4-{3-(2-[(N-tert-butoxycarbony-N-methyl)amιno]ethoxy)phenyl}-3-methylρhenyl)- piperidin- 1 -ylsulphonyl]-2-methylpropanamide 0-(7-Azabenzotnazol-l-yl)-N,N,N'N'-tetramethyluronιum hexafluorophosphate (540mg, 1.42mmol) was added to a solution of the acid from preparation 81 (520mg, 0.90mmol) and N- ethyldhsopropylamine (193μl, 1.12mmol) in N-methylpyπohdinone (10ml), and the reaction stined at room temperature under a nitrogen atmosphere for 40 m utes. Hydroxylamine hydrochloride (210mg, 3.02mmol) and additional N-ethyldiisopropylamine (730μl, 4.23mmol) were added, and the reaction stined at room temperature overnight. The mixture was partitioned between ethyl acetate and pH 7 buffer solution, and the layers separated. The organic phase was washed consecutively with water, brine, then dried (NaS0 4 ), filtered and evaporated in vacuo. The crude product was purified by medium pressure column chromatography on silica gel using an elution gradient of dichloromethane:methanol (99.5:0.5 to 98:2 to 80:20) to afford the title compound, (180mg, 34%). 'H nmr (CDC1 3 , 400MHz) δ: 1.40 (s, 9H), 1.63 (s, 6H), 1.78 (m, 2H), 1.86 (m, 2H), 2.22 (s, 3H), 2.61 (m, IH), 2.97 (s, 3H), 3.03 (m, 2H), 3.58 ( , 2H), 3.94 (m, 2H), 4.08 (m, 2H), 6.60 (s, IH), 6.64 (m, 2H), 7.02 (m, 2H), 7.17 (d, IH), 7.26 (dd, IH), 8.99 (s, IH), 10.75 (s, IH). Anal. Found: C, 60.96; H, 7.33; N, 7.11. C 30 H 43 N 3 0 7 S requires C, 61.10; H, 7.35; N, 7.12%. Preparation 89 N-Hydroxy 2-[4-(4-{3-(2-[(tert-butoxycarbonyl)amιno]ethoxy)phenyl}-3-methylphenyl)-pιpeπdm-l- ylsulphonyl]-2-methylpropιonamιde The title compound was obtamed (49%) from the acid from preparation 82, following a similar procedure to that descnbed in preparation 88 Η nmr (DMSO-d 6 , 400MHz) δ 1 37 (s, 9H), 1 48 (s, 6H), 1 60 (m, 2H), 1 79 (m, 2H), 2 20 (s, 3H), 2 64 (m, IH), 3 04 (m, 2H), 3 28 (m, 2H), 3 75 (m, 2H), 3 98 (t, 2H), 6 80-6 98 (m, 4H), 7 10 (s, 2H), 7 15 (s, IH), 7 30 (dd, IH), 8 99 (s, IH), 10 55 (s, IH) LRMS m/z 598 (M+23) + Anal Found C, 59 25, H, 7 09, N, 7 38 C, 9 H 4I N 3 O 7 S,0 1CH 2 C1 2 requires C, 59 83, H, 7 11, N, 7 19% Preparation 90 N-Hydroxy 4-[4-(4-{3-(2-[(N-tert-butoxycarbonyl)ammo]ethoxy)phenyl}-3-methylphenyl)-pιpendm-l- ylsulphonyl]-tetrahydro-2H-pyran-4-carboxamιde l-(3-Dιmethylammopropyl)-3-ethylcarbodnmιde hydrochloride (260mg, 1 36mmol) and l-hydroxy-7- azabenzotnazole (150mg, 1 lmmol) were added to a solution of the acid from preparation 84 (620mg, 1 03mmol) in pyndine (2ml) and dichloromethane (6ml), and the mixture stined at room temperature for 30 mmutes Hydroxylamme hydrochlonde (155mg, 2 25mmol) was added and the reaction stined at room temperature for 18 h The reaction mixture was partitioned between ethyl acetate and pH 7 buffer solution, and the layers separated The aqueous phase was extracted with ethyl acetate, the combmed organic solutions washed again with pH 7 buffer solution, then brine, dπed (Na 2 S0 4 ), filtered and evaporated m vacuo The residue was azeotroped with toluene, and then punfied by medium pressure column chromatography on silica gel using an elution gradient of dichloromethane methanol (100 0 to 90 10) The product was recrystallised from ediyl acetate/pentane to afford the title compound as a solid, (340mg, 53%) mp 181-182°C 'H nmr (DMSO-d,, 400MHz) δ 1 35 (s, 9H), 1 60 (m, 2H), 1 78 (m, 2H), 1 90 (m, 2H), 2 19 (s, 3H), 2 28 (m, 2H), 2 61 (m, IH), 3 02 (m, 2H), 3 20 (m, 2H), 3 22 (m, 2H), 3 70 (m, 2H), 3 84 (m, 2H), 3 98 (t, 2H), 6 79-6 95 (m, 4H), 7 08 (s, 2H), 7 15 (s, IH), 7 28 (m, IH), 9 10 (s, IH), 10 93 (s, IH) LRMS m z 640 (M+23) + Anal Found C, 60 27, H, 7 04, N, 6 63 C 3 ,H 43 N 3 0 8 S requues C, 60 27, H, 7 02, N, 6 88% Preparation 91 N-Hydroxy 4-[4-(4-{3-(N-tert-butoxycarbonyl-N-methyl)ammomethyl)phenyl}-3-methylphenyl)- pipendm- 1 -ylsulphonyl]-tetiahydro-2H-pyran-4-carboxamιde l-(3-Dunethylammopropyl)-3-ethylcarbodιιmιde hydrochloπde (216mg, 1 12mmol) and l-hydroxy-7- azabenzotπazole (128mg, 0 94mmol) were added to a solution of the acid from preparation 85 (550mg, 0 94mmol) m pyndine (2ml) and N,N dimethylformamide (6ml), and the mixture stined at room temperature for 1 hour Hydroxylamme hydrochloπde (195mg, 2 82mmol) was added and the reaction stured at room temperature overnight The reaction mixture was partitioned between ethyl acetate and pH 7 buffer solution, and the layers separated The aqueous phase was extracted with ethyl acetate (x2), the combmed organic solutions washed with 2N hydrochloπc acid, dned (MgS0 4 ), filtered and evaporated m vacuo The residue was crystallised from methanol/ethyl acetate to afford the title compound as a solid, (393mg, 70%) Η nmr (DMSO-d,, 400MHz) δ 1 36 (s, 9H), 1 59 (m, 2H), 1 78 (m, 2H), 1 88 (m, 2H), 2 18 (s, 3H), 2 27 (m, 2H), 2 61 (m, IH), 2 76 (s, 3H), 3 00 (m, 2H), 3 18 (m, 2H), 3 68 (m, 2H), 3 82 (m, 2H), 4 38 (s, 2H), 7 09 (m, 3H), 7 18 (m, 3H), 7 38 (m, IH), 9 10 (s, IH), 10 92 (s, IH) LRMS m/z 624 (M+l) + Preparation 92 1 -(4-Bromo-2-methylphenyl) 1 H-pyτazol-3-ol Potassium tert-butoxide (20ml, 1M m tert-butanol, 20 Ommol) was added to l-(4-bromo-2- methylphenyl)hydrazme (J Chem Soc 109, 1916, 582)(2 Olg, 10 Ommol) to give a dark brown suspension Ethyl propiolate (1 02ml, 1 Ommol) was then added dropwise over 10 minutes, with cooling, and once addition was complete, the reaction was heated under reflux for 4 hours The reaction was diluted with water (200ml) and this mixture washed with dichloromethane (2x50ml) The aqueous phase was acidified usmg hydrochlonc acid (2N), extracted with dichloromethane (5x100ml), these combmed organic extracts dned (MgS0 4 ), filtered and evaporated m vacuo The crude product was punfied by column chromatography on silica gel usmg dichloromethane methanol (98 2) as eluant, and tnturated with ether/di-isopropyl ether to give the title compound (615mg, 24%) as a solid mp 208-210°C Η nmr (DMSO-d*, 400MHz) δ 2 26 (s, 3H), 5 75 (s, IH), 7 22 (d, IH), 7 44 (d, IH), 7 57 (s, IH), 7 74 (s, IH), 10 00 (s, IH) LRMS m z 253, 255 (M+l) + Anal Found C, 47 31, H, 3 52, N, 10 99 C^^rN.O requires C, 47 46, H, 3 58, N, 11 07% Preparation 93 1 -(4-Bromo-2-methylphenyl)-3-methoxy- 1 H-pyrazole A mixture of the pyrazole from preparation 92 (1 52g, 6 Ommol), potassium carbonate (828mg, 6 Ommol), and dimethylsulphate (624ml, 6 6mmol) m l-methyl-2-pynohdmone (15ml) was heated at 90°C for 5 hours Tic analysis showed startmg matenal remammg, so additional potassium carbonate (828mg, 6 Ommol) and dimethylsulphoxide (624 9 1, 6 6mmol) were added, and stirring contmued at 90°C for a further 18 hours The cooled reaction was poured mto water (200ml), and the mixture exfracted with ethyl acetate (3x100ml) The combined organic extracts were washed with brine (3x100ml), dried (MgS0 4 ), filtered and concentrated in vacuo The residue was purified by column chromatography on silica gel using dichloromethane as die eluant, to give the desired product as a pale yellow oil, (970mg, 61%) Η nmr (CDC1 3 , 400MHz) δ 2 30 (s, 3H), 3.95 (s, 3H), 5.30 (s, IH), 5 85 (s, IH), 7 19 (d, IH), 7.38 (m, IH), 7 43 (s, IH) LRMS . m/z 267, 269 (M+l) + Preparation 94 l-(4-Bromo-2-methylρhenyl)-3-(2-hydroxyethoxy)-lH-pyrazole 2-Bromoethanol (1.55ml, 21 8mmol) was added to a mixture of the alcohol from preparation 92 (2.76g, 10.9mmol) and potassium carbonate (3.01g, 21.8mmol) m N,N-dιmethylformamιde (50ml), and the reaction stined at 80°C for 5 hours The cooled mixture was concentiated in vacuo, the residue suspended m ethyl acetate (250ml), and the mixture washed with water (5x50ml). The organic phase was dned (MgS0 4 ), filtered and evaporated in vacuo The crude product was punfied by column chromatography on silica gel using dιchloromethane:ether (80.20) as eluant, and crystallised from di-isopropyl ether to give the desired product as buff-coloured crystals, (1.61g, 50%). mp 104-105°C Η nmr (CDC1 3 , 400MHz) δ. 2.24 (s, 3H), 2.58 (br, s, IH), 3.92 (m, 2H), 4.36 (t, 2H), 5.84 (d, IH), 7.15 (d, IH), 7.35 (m, 2H), 7.40 (s, IH) Anal Found C, 48.38, H, 4.30, N, 9.34. C 12 H 13 BrN 2 0 2 requires C, 48.50; H, 4.41; N, 9.43%. Preparation 95 3-(2-Benzyloxyethoxy)- 1 -(4-bromo-2-methylphenyl)- 1 H-pyrazole A solution of the alcohol from preparation 94 (1.55g, 5.2mmol) m tetrahydrofuran (12ml) was added to a suspension of sodium hydπde (229mg, 60% dispersion m mineral oil, 5.73mmol) m tetrahydrofuran (10ml), and the resulting mixture stined for 2 mmutes under a nitiogen atmosphere Benzyl bromide (681μl, 5 73mmol) was then added and the reaction heated under reflux for 16 hours The cooled reaction mixture was poured into brine (70ml) and extracted with ethyl acetate (3x50ml) The combined organic solutions were dried (MgS0 4 ), filtered and concentrated in vacuo to give a yellow oil The crude product was purified by column chromatography on silica gel using an elution gradient of hexane ethyl acetate (90.10 to 80.20) to give the title compound as a colourless oil, (1 93g, 96%) Η nmr (CDC1 3 , 400MHz) δ 2 24 (s, 3H), 3 80 (t, 2H), 4 38 (t, 2H), 4 60 (s, 2H), 5 66 (s, IH), 7.12 (d, IH), 7 21 (m, 2H), 7 32 (m, 5H), 7 40 (s, IH) LRMS m/z 409, 411 (M+23) + Preparation 96 3-Methoxy- 1 -[(2-methyl-4-tnmethylstannyl)phenyl]- 1 H-pyrazole Tetrakιs(tnphenylphosphιne)ρalladιum (0) (30mg, 0 026mmol) was added to a solution of the bromide from preparation 93 (659mg, 2.47mmol), and hexamethylditin (889mg, 2Jlmmol) in 1,4-dioxan (8ml), and nitiogen bubbled through the resulting mixture. The reaction was heated under reflux for 4 A hours, then tic analysis showed startmg matenal remammg Additional tetrakιs(tnphenylphosphιne)palladιum (0) (48mg) was added and the reaction heated under reflux for a further 16 hours 50% Aqueous potassium fluonde solution (5ml) was added to the cooled reaction, the mixture stured for 15 mmutes, then filtered through Arbocel®, washmg through with ether The filtrate was washed with bnne (30ml), dπed (MgS0 4 ), filtered and evaporated m vacuo. The crude product was puπfied by column chromatography on silica gel usmg pentane:ether (90: 10) as eluant to give die title compound as a pale yellow oil, (598mg, 69%). Η nmr (CDC1 3 , 400MHz) δ: 0.27 (s, 9H), 2.26 (s 3H), 3.92 (s, 3H), 5.80 (s, IH), 7 21 (m, 2H), 7.35 (m, 2H). Preparation 97 3-(2-Benzyloxyethoxy)-l-[2-methyl-4-(tπmethylstannyl)phenyl]-lH-pyrazole Tetrakιs(tπphenylphosphιne)palladιum (0) (286mg, 0.25mmol) was added to a solution of the bromide from preparation 95 (1.92g, 4.96mmol), and hexamethylditin (1.78g, 5.45mmoI) m 1,4-dioxan (18ml), and nitrogen bubbled through the resulting mixture The reaction was heated under reflux for 2 hours, then cooled Potassium fluoride solution (5ml, 50%) was added, the mixture stined for 30 minutes, and filtered though Arbocel®, washing through well with ethyl acetate (150ml) The filtrate was washed with brine (2x30ml), dπed (MgS0 4 ), filtered and evaporated in vacuo The residue was purified by column chromatography on silica gel using hexane ether (84 16) to afford the desired product as a crystalline solid, (1 87g, 80%) mp 50-52°C Η nmr (CDC1 3 , 400MHz) δ 0 28 (s, 9H), 2 24 (s, 3H), 3 80 (t, 2H), 4 40 (t, 2H), 4 60 (s, 2H), 5 82 (s, IH), 7 22 (m, 3H), 7 33 (m, 6H) Anal Found C, 56 21, H, 5 97, N, 5 95 C 22 H 28 N 2 0 2 Sn requires C, 56 08, H, 5 99, N, 5 95% Preparation 98 Methyl 2-{4-[4-(3-methoxy-lH-pyrazol-l-yl}-3-methylphenyl]-l,2,3,6-tefrahydropyndm-l- ylsulphonyl}-2-methyl-propanoate Tπs(dιbenzyhdeneacetone)dιpalladιum(0) (30 7mg, 0 034mmol) was added to a solution of the vmyl tπflate from preparation 29 (727mg, 1 84mmol), the stannane from preparation 96 (590mg, 1 68mmol), and tnphenylarsine (104mg, 0 36mmol) m l-methyl-2-pynohdmone (4ml), and the solution stined under a nitrogen atmosphere Copper (I) iodide (16mg, 0 17mmol) was added, the solution de-gassed, and the reaction then stirred at 60°C for 30 mmutes, and at 75°C for a further 4 A hours Potassium fluoπde solution (3ml, 50%) was added to the cooled reaction, stirring continued for 15 mmutes, and the mixture filtered through Arbocel®, washmg through with ethyl acetate (150ml) The filtrate was washed with water (30ml), bnne (30ml), dπed (MgS0 4 ), filtered and evaporated m vacuo The residual orange foam was puπfied by column chromatography on silica gel usmg pentane ether (50 50) to afford the title compound as a pale yellow gum, (588mg, 81%) Η nmr (CDC1 3 , 400MHz) δ 1 63 (s, 6H), 2 30 (s, 3H), 2 59 (m, 2H), 3 60 (t, 2H), 3 79 (s, 3H), 3 94 (s, 3H), 4 08 (m, 2H), 5 81 (d, IH), 6 00 (m, IH), 7 21 (m, 3H), 7 36 (s, IH) LRMS m z 434 (M+l) + Preparation 99 Methyl 2- {4-[4-(3- {2-benzyloxyethoxy } - IH-pyrazol- 1 -yl}-3-methylphenyl]- 1 ,2,3,6-tetrahydropyridin- 1- ylsulphonyl}-2-medιyl-propanoate The title compound was obtained as a yellow oil (75%) from the triflate from preparation 29 and die stannane of preparation 97, using a similar method to that described in preparation 98. Η nmr (CDC1 3 , 400MHz) δ: 1.64 (s, 6H), 2.27 (s, 3H), 2.58 (m, 2H), 3.59 (m, 2H), 3.78 (s, 3H), 3.80 (t, 2H), 4.09 (m, 2H), 4.39 (t, 2H), 4.60 (s, 2H), 5.85 (s, 111), 6.00 (m, IH), 7.21 (m, 4H), 7.34 (m, 5H). LRMS : m/z 576 (M+23) + Preparation 100 Metiiyl 2- {4-[4-(3-methoxy- 1 H-pyrazol- 1 -yl} -3-methylphenyl]piperidin- 1 -ylsulphonyl} -2- methylpropanoate 10% Palladium on charcoal (60mg) was added to a solution of the 1,2,3,6-tetrahydropyridine from preparation 98 (580mg, 1.38mmol) in methanol (20ml), and the mixture hydrogenated at 50 psi and room temperature for 6 hours. Tic analysis showed starting material remaining, so additional 10% palladium on charcoal (50mg) was added, and the mixture hydrogenated for a further 18 hours. The reaction mixture was filtered through Arbocel®, the filtrate suspended in dichloromethane (50ml), re-filtered through Arbocel®, and the filtrate evaporated in vacuo, to give the desired product as a colourless solid, (365mg, 61%). mp l09-110°C 'H nmr (CDC1 3 , 400MHz) δ: 1.61 (s, 6H), 1.75-1.86 (m, 4H), 2.25 (s, 3H), 2.62 (m, IH), 3.02 (m, 2H), 3.79 (s, 3H), 3.94 (m, 5H), 5.80 (d, IH), 7.06 (m, 2H), 7.21 (m, 2H). LRMS : m/z 458 (M+23) + Preparation 101 Methyl 2- {4-[4-(3- (2-hydroxyethoxy } - 1 H-pyrazol- 1-yl} -3-methylphenyl]piperidin- 1 -ylsulphonyl}-2- methylpropanoate A mixture of the benzyl ether from preparation 99 (790mg, 1.42mmol) and 10% palladium on charcoal (160mg) in ethanol (35ml) was hydrogenated at 50 psi and room temperature for 17 hours. Tic analysis showed starting material remaining, so acetic acid (2ml), and additional 10% palladium on charcoal (80mg) were added, and the reaction continued for a further 48 hours, with additional 10% palladium on charcoal (160mg) added portionwise. The reaction mixture was filtered through Arbocel®, washing through with ethanol, and the filtrate concentrated in vacuo. The residue was partitioned between ethyl acetate (100ml) and saturated sodium bicarbonate solution (100ml), the layers separated and the organic phase dried (MgS0 4 ), filtered and evaporated in vacuo to give the title compound as a colourless oil, (630mg, 95%). Η nmr (DMSOΛ, 400MHz) δ: 1.46-1.62 (m, 8H), 1.80 (m, 2H), 2.19 (s, 3H), 2.71 (m, IH), 3.02 (m, 2H), 3.10 (m, 2H), 3.62-3.79 (m, 5H), 4.10 (m, 2H), 4.60 (m, IH), 5.84 (s, IH), 7.12 (m, IH), 7.19 (m, 2H), 7.69 (s, IH). LRMS : m z 488 (M+23) + Preparation 102 Metiiyl 2-methyl-2- {4-[3-methyl-4-( 1 ,3-thiazol-2-yl)phenyl]piperidin- 1 -ylsulphonyl} -propanoate Bis(triphenylphosphine)palladium (II) chloride (49mg, 0.07mmol) was added to a solution of the bromide from preparation 26 (577mg, 1.38mmol) and 2-(trimethylstannyl)-l,3-thiazole (Synthesis, 1986, 757) (372mg, 1.5mmol) in tetrahydrofuran (3.5ml), and the resulting mixture was de-gassed, and placed under an argon atmosphere. The reaction was heated under reflux for 17 hours. Tic analysis showed starting material remaining, so additional 2-(trimethylstannyl)-l,3-thiazole (173mg, 0.8m ol) and bis(triphenylphosphine)palladium (II) chloride (49mg, 0.07mmol) were added, the mixture was degassed, and dien heated under reflux for a further 17 hours. The cooled mixture was concentiated in vacuo, and die residue purified by column chromatography on silica gel using an elution gradient of hexane:ethyl acetate (91 :9 to 66:34). The product was re-purified by column chromatography on silica gel using ether as eluant to give the title compound as a buff-coloured solid, (240mg, 40%). mp l l l-1 14°C Η nmr (DMSO-d,,, 400MHz) δ: 1.52 (s, 6H), 1.58 (m, 2H), 1.81 (m, 211), 2.45 (s, 3H), 2.74 (m, IH), 3.04 (m, 2H), 3.74 (m, 5H), 7.18 (d, IH), 7.21 (s, IH), 7.62 (d, IH), 7.78 (d, IH), 7.92 (d, IH). LRMS : m/z 445 (M+23) + Anal. Found: C, 56.64; H, 6.19; N, 6.55. C 20 H 26 N 2 S 2 O 4 requires C, 56.85; H, 6.20; N, 6.63%. Preparation 103 2- {4-[4-(3-Methoxy- 1 H-pyrazol- 1 -yl} -3-methylρhenyl]piperidin- 1 -ylsulphonyl} -2-methylpropanoic acid A mixture of the methyl ester from preparation 100 (355mg, 0.82mmol), and aqueous sodium hydroxide (5.9ml, 1M, 5.9mmol) in methanol (5ml) and 1,4-dioxan (5ml) was heated under reflux for 2 hours. The cooled reaction was diluted with water and acidified to pH 3 using hydrochloric acid (2N). The resulting precipitate was filtered off, washed with water, and dried under vacuum at 75 °C to give the title compound as a white powder, (281mg, 82%). Η nmr (CDC1 3 , 400MHz) δ: 1.63 (s, 6H), 1.70-1.90 (m, 4H), 2.24 (s, 3H), 2.62 (m, IH), 3.04 (m, 2H), 3.90 (s, 3H), 3.98 (m, 2H), 5.80 (s, IH), 7.04 (m, 3H), 7.32 (m, IH). Anal. Found: C, 56.78; H, 6.40; N, 9.71. C 20 H 27 N 3 O 5 S requires C, 56.99; H, 6.46; N, 9.97%. Preparation 104 2- {4-[4-(3- {2-Hydroxyethoxy } - 1 H-pyrazol- 1 -yl} -3-methylphenyl]pιpeπdιn- 1 -ylsulphonyl} -2- methylpropanoic acid Me Me A mixture of the methyl ester from preparation 101(520mg, 1.2mmol), and aqueous sodium hydroxide (3 6ml, 1M, 3 όmmol) in 1 ,4-dioxan (5ml) was heated under reflux for 2 X A hours The cooled reaction was partitioned between water (100ml) and ethyl acetate (100ml), acidified to pH 2 using hydrochloric acid (2N), and the phases separated The aqueous layer was extracted with ethyl acetate (2x35ml), the combined organic solutions dned (MgS0 4 ), filtered and concentrated m vacuo. The residue was tnturated with ether twice, to afford the title compound as a white solid, (338mg, 62%) 'H nmr (DMSO-d^, 300MHz) δ 1 47 (s, 6H), 1.59 (m, 2H), 1.79 (m, 2H), 2.19 (s, 3H), 2.70 (m, IH), 3.02 (m, 2H), 3.64 (m, 2H), 3J9 (m, 2H), 4 09 (t, 2H), 4.62 (m, IH), 5.84 (s, IH), 7.12 (m, IH), 7.18 (m, 2H), 7.69 (s, IH), 13.1 (br, s, IH) LRMS : m z 474 (M+23) + Preparation 105 2-Medιyl-2-{4-[3-methyl-4-(l,3-thιazol-2-yl)phenyl]pιpeπdm-l-ylsulphonyl}-propanoιc acid The title compound was obtamed as a white solid (92%) from the methyl ester of preparation 102, followmg a similar procedure to that described m preparation 104. "H nmr (DMSO-d,, 400MHz) δ 1 47 (s, 6H), 1.60 (m, 2H), 1.80 (m, 2H), 2.45 (s, 3H), 2.70 (m, IH), 3.03 (m, 2H), 3 78 (m, 2H), 7 18 (d, IH), 7 21 (s, IH), 7 63 (d, IH), 7.78 (s, IH), 7.92 (s, IH), 13.37 (br, s, lH) Anal Found. C, 55.28, H, 5 90, N, 6 70 C 19 H 24 N 2 0 4 S 2 requires C, 55 86, H, 5.92, N, 6 86% Preparation 106 Methyl 1 - { [4-(4-bromo-3-methylphenyl)pιpeπdιn- 1 -yljsulfonyl} -3-cyclopentene- 1 -carboxylate A suspension of sodium hydride (l. lg, 60% dispersion in mineral oil, 28mmol) was cooled to 0°C m anhydrous N-methyl pyπohdinone (30ml) under nitiogen A solution of the ester from preparation 25 (lOg, 26mmol) m N-methyl pynolidmone (70ml) was added dropwise with stirring and the reaction mixture allowed to warm to ambient temperature over 50 mmutes 1 ,4-dιchlorobut-2-ene (3 0ml, 28mmol) and tetrabutylammonium bromide (8.3g, 26mmol) were added to the reaction mixture and after a further 3 hours an additional portion of sodium hydπde (l.lg, 60% dispersion m mmeral oil, 28mmol) was added The mixture was stured for a further 2 days. The reaction mixture was partitioned between ethyl acetate (300ml) and water (300ml) and the layers separated. The aqueous layer was exfracted with ethyl acetate (300ml) and the combmed organic extracts were dned (Na 2 S0 4 ), filtered and concentiated m vacuo The residue was punfied by flash chromatography elutmg with dichloromethane to give the title compound as a white solid (7.4g, 65%). 'H nmr (DMSO-cl*, 400MHz) δ: 1.45 (m, 2H), 1.75 (m, 2H), 2.28 (s, 3H), 2.64 (m, IH), 2.95 (m, 4H), 3.14 (d, 2H), 3.75 (s, 3H), 3.78 (s, 2H), 5.63 (s, 2H), 6.98 (d, IH), 7.21 (s, IH), 7.43 (d, IH). LRMS :m/z 464/466 (M+23) + . Preparation 107 Methyl (lα,3 ,4α)-l-{ [4-(4-bromo-3-methylρhenyl)pipendm- 1 -yl] sulfony 1} -3 ,4- dihydroxycyclopentanecarboxylate N-methylmo hohne N-oxide (580mg, 4 97mmol) and osmium tetroxide (2 5 weight % in tert-butanol, 1 1ml, 0 136mmoI) were added to a solution of die cyclopentene from preparation 106 (2 Og, 4 52mmol) m dioxan (20ml), water (0 1ml), and die solution stined at room temperature for 18 hours The reaction mixture was partitioned between ethyl acetate (200ml) and water (300ml) and the layers separated The aqueous layer was extiacted with ethyl acetate (2x200ml), and the combined organic extracts were dπed (Na 2 S0 4 ), filtered and concentrated m vacuo The residue was purified by column chromatography on silica gel usmg dichloromethane/methanol (100 0 to 97 3) as eluant to afford the title compound as a white solid (1 2g, 56%) Η nmr (DMSO-d 6 , 400MHz) δ 1 47 (m, 2H), 1 77 (m, 2H), 2 28 (m, 5H), 2 42 (s, 2H), 2 63 (m, IH), 2 91 (m, 2H), 3 75 (m, 5H), 3 85 (s, 2H), 4 62 (s, 2H), 6 98 (d, IH), 7 21 (s, IH), 7 43 (d, IH) LRMS m/z 498/500 (M+23) + Preparation 108 Methyl (lα,3β,4β)-l-{ [4-(4-bromo-3 -methylpheny l)pιpendm- 1 -yl] sulfonyl } -3 ,4- dihydroxycyclopentanecarboxylate Silver acetate (2 lg, 12 46mmol) and lodme (1 5g, 5 δlmmol) were added to a solution of the cyclopentene from preparation 106 (2 45g, 5 54mmol) m glacial acetic acid (125ml) and the mixture was stured at ambient temperature for 1 hour Wet acetic acid (2 5ml of a 1 25 water/glacial acetic acid mixture) was then added and the reaction was heated to 95°C for 3 hours and then stured at ambient temperature for 18 hours Sodium chlonde was added to the mixture and the resulting precipitate was filtered through arbocel® and then washed with toluene The resultmg filtrate was concentiated m vacuo, azeotroped with toluene to give a solid which was tnturated with diisopropyl ether This solid was further punfied by flash chromatography elutmg with dichloromethane to give the intermediate monoacetate compound as a beige solid (1 35g, 50%) IN sodium hydroxide (4ml) was added to a solution of the monoacetate intermediate m dioxan/methanol (12ml/8ml) and the reaction was stined at ambient temperature for 1 hour The solvent was removed under reduced pressure, and the residue was partitioned between ethyl acetate (50ml) and water (75ml), and the layers separated The aqueous layer was extracted with ethyl acetate (2x50ml), and the combmed organic extracts were dned (Na 2 S0 4 ), filtered and concentiated in vacuo to give the title compound as a white solid (875mg, 70%) 'H nmr (DMSO-d 6 , 400MHz) δ: 1.55 (m, 2H), 1.87 (m, 2H), 2.18 (m, 2H), 2.30 (s, 3H), 2.63 (m, 3H), 2.98 (t, 2H), 3.72 (m, 7H), 4.68 (s, 2H), 6.98 (d, IH), 7.22 (s, IH), 7.43 (d, IH). LRMS :m/z 498/500(M+23)\ Preparation 109 Metiiyl (3aα,5α,6aα)-5-{[4-(4-bromo-3-methylphenyl)piperidin-l-yl]sulfonyl}-2,2-dimethyltetrahydro- 3a/ -cyclopenta[J][l,3]dioxole-5-carboxylate 2,2-Dimethoxypropane (0.74ml, 6mmol) and p-toluenesulfonic acid (60mg, 0.3mmol) were added to a solution of the diol from preparation 107 (1.43g, 3mmol) in anhydrous dimethylformamide (10ml) under nitiogen. The reaction was warmed to 50°C for 4.5hours. The mixture was diluted with ethyl acetate (50ml) and water (40ml) and the layers separated. The aqueous layer was exfracted with ethyl acetate (2x50ml), and the combined organic extracts were dried (Na 2 S0 4 ), filtered and concenfrated in vacuo. The resulting solid was recrystalised from ethyl acetate/di-isopropyl ether to give the title compound as a white solid (1.05g, 70%). Η nmr (DMSO-d β , 400MHz) δ: 1.17 (s, 3H), 1.20 (s, 3H), 1.47 (m, 2H), 1.77 (m, 2H), 2.23 (m, 2H), 2.32 (s, 3H), 2.65 (m, 3H), 2.95 (t, 2H), 3.72 (m, 5H), 4.64 (s, 2H), 6.98 (d, IH), 7.21 (s, IH), 7.43 (d, IH). LRMS :m/z 538/540 (M+23) + . Preparation 110 Methyl (3aβ,5α,6aβ)-5-{[4-(4-bromo-3-methylphenyl)piperidin-l-yl]sulfonyl}-2,2-dimethyltetiahydro- 3aH-cyclopenta[d][l,3]dioxole-5-carboxylate The title compound was prepared from the diol from preparation 108 m a similar procedure to that descπbed m preparation 109 The title compound was isolated as a pale yellow solid (1 3g, 75%) "H nmr (DMSO-dβ, 400MHz) δ 1 11 (s, 3H), 1 42 (s, 3H), 1 57 (m, 2H), 1 78 (m, 2H), 2 18 (m, 2H), 2 30 (s, 3H), 2 62 (m, IH), 2 78 (m, 2H), 2 98 (t, 2H), 3 72 (m, 5H), 4 58 (m, 2H), 6 98 (d, IH), 7 22 (s, IH), 7 43 (d, IH) LRMS m/z 538/540(M+23) + Preparation 111 Methyl (3aα,5α,6aα)-5-{[4-(4-{6-[2-(rer/-butoxy)ethoxy]pyπdm-2-yl}-3-methylphenyl)pιpeπdm-l- yl]sulfonyl}-2,2-dunethyltefrahydro-3a//-cyclopenta[tTl[l,3]dioxole-5-carboxylate A mixture of the stannane from preparation 127 (2 3g, 4 78mmol) and the aryl bromide from preparation 109 (1 9g, 3 68mmol), and tetrakιs(tnphenylphosρhιne)palladιum (0) (213mg, 0 18mmol) in toluene (25ml) was refluxed under nitrogen for 10 hours, then stined at ambient temperature for 7 hours The mixture was evaporated m vacuo and to the resultmg oil was added ethyl acetate (30ml) and aqueous potassium fluonde solution (20ml) and stined rapidly for 10 minutes The resulting precipitate was filtered off on arbocel® washmg with ethyl acetate The filtrate was allowed to separate, and the aqueous layer extracted with ethyl acetate (30ml) The combmed organic extracts were dned (Na 2 S0 4 ), filtered and concenfrated in vacuo The residue was purified by column chromatography on silica gel using pentane ethyl acetate (98 2 to 60 40) as eluant The resulting solid was recrystalised from ethyl acetate to afford the title compound as a white solid, (1 4g, 60%) Η nmr (DMSO-d«, 400MHz) δ 1 13 (s, 9H), 1 17 (s, 3H), 1.20 (s, 3H), 1.57 (m, 2H), 1.80 (m, 2H), 2 23 (m, 2H), 2 32 (s, 3H), 2 69 (m, 3H), 2 95 (t, 2H), 3 60 (m, 2H), 3 72 (m, 5H), 4 29 (m, 2H), 4 68 (s, 2H), 6 73 (d, 111), 7 03 (d, IH) 7 15 (m, 2H), 7.31 (d, IH), 7.75 (t, IH) LRMS m z 654 (M+23) + Preparation 1 12 Methyl (3aα,5α,6aα)-5-({4-[4-(6-ethoxypyπdιn-2-yl)-3-methylphenyl]pιpeπdm-l-yl}sulfonyl)-2,2- dιmetiιyltefrahydro-3aN-cyclopenta[ f|[l,3]dιoxole-5-carboxylate The title compound was prepared from the aryl bromide from preparation 109 and the stannane from preparation 129 in a similar procedure to that descnbed in preparation 111. The title compound was isolated as a white solid (l.lg, 50%) 'H nmr (DMSO-d* 400MHz) δ 1.15 (s, 3H), 1.19 (s, 3H), 1.25 (t, 3H), 1.57 (m, 2H), 1.80 (m, 2H), 2.23 (m, 2H), 2.35 (s, 3H), 2.65 (m, 3H), 2.95 (t, 2H), 3.65 (m, 2H), 3.72 (m, 3H), 4.28 (q, 2H), 4.66 (d, 2H), 6.68 (d, IH), 7.03 (d, IH), 7.15 (m, 2H), 7.33 (d, IH), 7J2 (t, IH). LRMS :m/z 581 (M+23) + . Preparation 113 Methyl (3aβ,5α,6aβ)-5-({4-[4-(6-ethoxypyndιn-2-yl)-3-methylphenyl]pιpendm-l-yl}sulfonyl)-2,2- dιmemyltetiahydro-3aN-cyclopenta[d][l,3]dιoxole-5-carboxylate The title compound was prepared from the aryl bromide from preparation 110 and the stannane from preparation 129 in a similar procedure to that described in preparation 111. The title compound was isolated as a white foam (413mg, 60%). 'H nmr (DMSO-d,, 400MHz) δ: 1.21 (s, 3H), 1.28 (t, 3H), 1.42 (s, 3H), 1.57 (m, 2H), 1.80 (m, 2H), 2.18 (m, 2H), 2.35 (s, 3H), 2.65 (m, IH), 2.80 (m, 2H), 3.00 (t, 2H), 3.75 (m, 2H), 3.77 (s, 3H), 4.28 (q, 2H), 4.56 (m, 2H), 6.68 (d, IH), 7.03 (d, IH), 7.15 (m, 2H), 7.35 (d, IH), 7.72 (t, IH). LRMS :m/z 559 (M+l) + . Preparation 114 Methyl (3aα,5α,6aα)-5-{4-[4-(3-methoxyphenyl)-3-methylρhenyl]piperidin-l-ylsulfonyl}-2,2- dimethyltettahydro-3ai/-cyclopenta[ ][l,3]dioxole-5-carboxylate A mixture of the aryl bromide from preparation 109 (1.03, 1.99mmol), 3-methoxyphenylboronic acid (364mg, 2.40mmol), cesium fluoride (606mg, 4.00mmol), fris(dibenzylideneacetone)dipalladium'(0) (91mg, 0.1 mmol) and tri(o-tolyl)phosphine (61mg, 0.2mmol) in 1,2-dimethoxyethane (25ml) was heated under reflux under nitrogen for 9 hours. The cooled reaction was diluted with water and ethyl acetate, filtered through arbocel®, which was washed with water and ethyl acetate. The organic layer was separated, and washed with brine, dried (Na 2 S0 4 ), filtered and concentrated in vacuo. The residue was purified by column chromatography on silica gel using pentane:ethyl acetate (95:5 to 60:40) as eluant. The title compound was obtained as a white solid (630mg, 60%). WO 00/74681 , , , PCT/IB 116 'H nmr (DMSO-d 6 , 400MHz) δ: 1.15 (s, 3H), 1.18 (s, 3H), 1.57 (m, 2H), 1.79 (m, 2H), 2.18 (m, 511), 2.65 (m, 3H), 2.95 (t, 2H), 3.65 (m, 8H), 4.64 (m, 2H), 6.82 (m, 3H), 7.10 (m, 3H), 7.29 (m, IH). LRMS :m/z 566 (M+23) + . Preparation 1 15 Methyl (3aβ,5α,6aβ)-5-{4-[4-(3-memoxyphenyl)-3-methylphenyl]piperidin-l-ylsulfonyl}-2,2- dimethyltefrahydro-3aN-cyclopenta[tz][l,3]dioxole-5-carboxylate The title compound was prepared from the aryl bromide from preparation 110 in a similar procedure to that described in preparation 114 and was isolated as a white foam (310mg, 45%). Η nmr (DMSO-d,, 400MHz) δ: 1.20 (s, 3H), 1.40 (s, 3H), 1.57 (m, 2H), 1.80 (m, 2H), 2.18 (m, 5H), 2.67 (m, IH), 2.81 (m, 2H), 2.95 (t, 2H), 3.75 (m, 8H), 4.57 (m, 2H), 6.82 (m, 3H), 7.10 (m, 3H), 7.29 (m, IH). LRMS :m/z 566 (M+23) + . Preparation 116 (3aα,5α,6aα)-5-{[4-(4-{6-[2-(rert-butoxy)ethoxy]pyridin-2-yl}-3-methylphenyl)piperidin-l- yl]sulfonyl}-2,2-dimethyltefrahydro-3aN-cyclopenta[d][l,3]dioxole-5-carboxylic acid A mixture of the methyl ester from preparation 1 11 (1.4g, 2.22mmol) and aqueous sodium hydroxide (5.5ml, 2N, 11. lmmol) in methanol (7ml) and dioxan (7ml) was heated under reflux for lhour, then allowed to cool The reaction was concentrated m vacuo, the residue dissolved in water (20ml), and the solution acidified to pH 4 with glacial acetic acid. The aqueous was extiacted with etiiyl acetate (2x 50ml) and the collected organic layers dπed (Na 2 S0 4 ), filtered and concenfrated in vacuo. The resulting oily solid was azeotroped with toluene then tnturated with cold ethyl acetate to afford the title compound as a white solid (l.Og, 75%). Η nmr (DMSO-d 6 , 400MHz) δ: 1.13 (s, 9H), 1.16 (s, 3H), 1.28 (s, 3H), 1.57 (m, 2H), 1.75 (m, 2H), 2.26 (m, 5H), 2.59 (m, 3H), 3.05 (t, 2H), 3.60 (m, 2H), 3.72 (d, 2H), 4.28 (m, 2H), 4.58 (m, 2H), 6.73 (d, IH), 7.03 (d, IH), 7.15 (m, 2H), 7.31 (d, IH), 7.75 (t, IH) 12.9 (s, IH). LRMS :m/z 617 (M+l) + . Preparation 117 (3aα,5α,6aα)-5-({4-[4-(6-ethoxypyπdm-2-yl)-3-methylphenyl]pιpendm-l-yl}sulfonyl)-2,2- dimethyltetrahydro-3aN-cyclopenta[cT] [ 1 ,3]dioxole-5-carboxyhc acid A mixture of the methyl ester from preparation 112 (780mg, 1.40mmol) and aqueous sodium hydroxide (3.5ml, 2N, 6.98mmol) were dissolved in methanol (5ml) and dioxan (5ml) and were heated under reflux for 1.5 hour, then allowed to cool. The reaction was concentrated in vacuo, the residue dissolved in water (20ml), and the solution acidified to pH 4 with glacial acetic acid. The resulting mixture was extracted with ethyl acetate (2x 50ml) and the collected organic layers dried (Na 2 S0 4 ), filtered and concentrated m vacuo. This afforded the title compound as a white solid (240mg, 85%). Η nmr (DMSO-d β , 400MHz) δ: 0.93 (s, 3H), 1.14 (m, 6H), 1.41 (m, 2H), 1.58 (m, 2H), 2.01 (m, 2H), 2.13 (s, 3H), 2.43 (m, 3H), 2.78 (m, 2H), 3.50 (m, 2H), 4.08 (m, 2H), 4.43 (m, 2H), 6.48 (m, IH), 6.80 (d, IH), 6.91 (m, 2H), 7.10 (m, IH), 7.51 (m, IH) 13.10 (s, IH). LRMS :m/z 545 (M+l) + . Preparation 1 18 (3aβ,5α,6aβ)-5-({4-[4-(6-ethoxypyrιdm-2-yl)-3-methylphenyl]pιpeπdm-l-yl}sulfonyl)-2,2- dιmethyltetiahydro-3a//-cyclopenta[J|[l,3]dιoxole-5-carboxylιc acid The title compound was prepared from the methyl ester from preparation 113 m similar procedure to that descnbed in preparation 117 and was isolated as a white foam (250mg, 65%) Η nmr (DMSO-d^, 400MHz) δ 1.21 (s, 3H), 1.28 (t, 3H), 1.42 (s, 3H), 1.61 (m, 2H), 1.80 (d, 2H), 2.18 (m, 2H), 2.35 (s, 3H), 2 65 (m, IH), 2 80 (m, 2H), 3.00 (t, 2H), 3.78 (d, 2H), 4.28 (q, 2H), 4 56 (m, 2H), 6.68 (d, IH), 7.01 (d, IH), 7.15 (m, 2H), 7 35 (d, IH), 7.72 (t, IH), 13.65 (s, IH). LRMS :m/z 545 (M+l) + . Preparation 119 (3aα,5α,6aα)-5-{4-[4-(3-methoxyphenyl)-3-methylphenyl]pιpendm-l-ylsulfonyl}-2,2- dιmethyltetiahydro-3aN-cyclopenta[rf][l,3]dιoxole-5-carboxylιc acid A mixture of the methyl ester from preparation 114 (630mg, l .lόmmol) and aqueous sodium hydroxide (3.0ml, 2N, 5.80mmol) were dissolved in methanol (5ml) and dioxan (5ml) and heated under reflux for lhour, then allowed to cool. The reaction was concentiated in vacuo, the residue dissolved m water (20ml), and the solution acidified to pH 1 with 2N hydrochloric acid The resultmg mixture was exfracted with ethyl acetate (2x 50ml) and the collected o ganic layers dried (Na 2 S0 4 ), filtered and concenfrated in vacuo. This afforded the title compound as a white solid (500mg, 83%) Η nmr (DMSO-d 6 , 400MHz) δ 1 13 (s, 3H), 1 22 (s, 3H), 1 58 (m, 2H), 1 79 (m, 2H), 2 18 (m, 5H), 2 62 (m, 3H), 2 97 (t, 2H), 3 71 (m, 5H), 4 64 (m, 2H), 6 82 (m, 311), 7 06 (m, 2H), 7 14 (s, IH), 7 29 (t, IH) LRMS m/z 528 (M-l) Preparation 120 (3aβ,5α,6aβ)-5-{4-[4-(3-medιoxyphenyl)-3-methylphenyl]pιpeπdιn-l-ylsulfonyl}-2,2- dιmethyltefrahydro-3a//-cyclopenta[ ][l,3]dιoxole-5-carboxylιc acid The title compound was prepared from the methyl ester from preparation 115 m a similar procedure to that descnbed m preparation 119 and was isolated as a white foam (250mg, 85%) Η nmr (DMSOA, 400MHz) δ 1 20 (s, 3H), 1 40 (s, 3H), 1 58 (m, 2H), 1 80 (m, 2H), 2 15 (m, 2H), 2 18 (s, 3H), 2 65 (m, IH), 2 78 (m, 2H), 2 99 (t, 2H), 3 77 (m, 5H), 4 56 (m, 2H), 6 82 (m, 3H), 7 10 (m, 3H), 7 29 (t, IH), 13 78 (s, IH) LRMS m/z 528 (M-l) Preparation 121 (3aα,5α,6aα)-Nhydroxy-5-{[4-(4-{6-[2-(/er/-butoxy)ethoxy]pyndm-2-yl}-3-methylphenyl)pιpeπdm-l- yl]sulfonyl}-2,2-dιmethyltefrahydro-3aN-cyclopenta[ T][l,3]dιoxole-5-carboxamιde l-(3-Dιmethylammopropyl)-3-ethylcarbodnmιde hydrochlonde (190mg, 0 973mmol) and l-hydroxy-7- azabenzotnazole (121mg, 0.892mmol) were added to a solution of the acid from preparation 116 (500mg, 0 81 lmmol) in N,N-dιmethylformamιde (6ml) and pyndine (3ml) and the reaction was stured under nitrogen for 50 mmutes Hydroxylamine hydrochloride (170mg, 2 43mmol) was then added, and the reaction stined at room temperature overnight The reaction was diluted with ethyl acetate (50ml) and washed with pH 7 phosphate buffer solution (30ml) The aqueous layer was exfracted with ethyl acetate (2x 50ml) and the combmed organic extracts were washed with bnne, then water, dned (Na 2 S0 4 ), filtered and concentiated m vacuo The resultmg solid was recrystallised from ethyl acetate to afford the title compound as a white solid (260mg, 50%). 'H nmr (DMSO-d*, 400MHz) δ: 1.15 (s, 9H), 1.16 (s, 3H), 1.20 (s, 3H), 1 59 (m, 2H), 1 75 (m, 2H), 2.17 (m, 2H), 2.31 (s, 3H), 2.59 (m, IH), 2.66 (d, 2H), 2.99 (t, 2H), 3.59 (m, 2H), 3 64 (d, 2H), 4.28 (m, 2H), 4.62 (m, 2H), 6.72 (d, IH), 7.03 (d, IH), 7.15 (m, 2H), 7.29 (d, IH), 7 70 (t, IH), 8 85 (s, IH), 10.82 (s, IH). LRMS :m/z 632 (M+l) + . Preparation 122 (3aα,5α,6aα)-Nhydroxy-5-({4-[4-(6-ethoxypyπdm-2-yl)-3-methylphenyl]pιpendιn-l-yl}sulfonyl)-2,2- dιmethyltefrahydro-3aN-cyclopenta[<f|[l,3]dιoxole-5-carboxamιde The title compound was prepared from the acid from preparation 1 17 in a similar procedure to that described in preparation 121, and was isolated as a white solid (150mg, 60%) Η nmr (DMSO-d,, 400MHz) δ 1 13 (s, 3H), 1 21 (s, 3H), 1 25 (t, 3H), 1 61 (m, 211), 1 76 (m, 2H), 2 18 (m, 2H), 2 32 (s, 3H), 2 60 (m, IH), 2 77 (d, 2H), 2 99 (t, 2H), 3 63 (d, 2H), 4 25 (q, 2H), 4 63 (m, 2H), 6 68 (d, IH), 7 02 (d, IH), 7 14 (m, 2H), 7 30 (d, IH), 7 71 (t, IH), 8 86 (s, IH), 10 82 (s, IH) LRMS m z 560 (M+l) + Preparation 123 (3aβ,5α,6aβ)-. /-hydroxy-5-({4-[4-(6-ethoxy-pyndιn-2-yl)-3-methylphenyl]pιpendιn-l-yl}sulfonyl)-2,2- dιmethyltefrahydro-3aN-cyclopenta[ ][l,3]dιoxole-5-carboxarmde The title compound was prepared from the acid from preparation 118 m a similar procedure to that descnbed m preparation 121 The title compound was isolated after column chromatography (usmg dichloromethane/methanol 99.1 as eluant) as a white solid (107mg, 45%). Η nmr (DMSO-d«. 400MHz) δ 1.20 (s, 3H), 1.28 (t, 3H), 1.40 (s, 3H), 1.61 (m, 2H), 1 80 (d, 2H), 2.05 (m, 2H), 2.30 (s, 3H), 2.62 (m, IH), 2.97 (m, 4H), 3 70 (d, 2H), 4.28 (q, 2H), 4.45 (m, 2H), 6 68 (d, IH), 7 01 (d, IH), 7 15 (m, 2H), 7.32 (d, IH), 7.72 (t, IH), 9.00 (s, IH), 10.39 (s, IH) LRMS :m/z 560 (M+l) + . Preparation 124 (3a ,5α,6aα)-/V-hydroxy-5-{4-[4-(3-methoxyphenyl)-3-methylphenyl]pιpendm-l-ylsulfonyl}-2,2- dιmethyltefrahydro-3aN-cyclopenta[rf][l,3]dιoxole-5-carboxamιde The title compound was prepared from the acid from preparation 119 in a similar procedure to that described in preparation 121, and was isolated as a white solid (1 lOmg, 43%). Η nmr (DMSO-d 6 , 400MHz) δ: 1.13 (s, 3H), 1.22 (s, 3H), 1.58 (m, 2H), 1.77 (m, 2H), 2.18 (m, 5H), 2.58 (m, IH), 2.75 (d, 2H), 2.98 (t, 2H), 3.65 (d, 2H), 3.75 (s, 3H), 4.63 (m, 2H), 6.82 (m, 3H), 7.08 (s, 2H), 7.15 (s, IH), 7.28 (t, IH), 8.85 (s, IH), 10.82 (s, IH). Preparation 125 (3aβ,5α,6aβ)-Λ r -hydroxy-5-{4-[4-(3-methoxyphenyl)-3-methylphenyl]piperidin-l-ylsulfonyl}-2,2- dimethyltetrahydro-3a//-cyclopenta[<Z][l,3]dioxole-5-carboxamide The title compound was prepared from the acid from preparation 120 in a similar procedure to that described in preparation 121. The title compound was isolated after column chromatography (using dichloromethane/methanol 98:2 as eluant) as a white solid (130mg, 50%). 'H nmr (DMSO-d^, 400MHz) δ: 1.20 (s, 3H), 1.40 (s, 3H), 1.58 (m, 2H), 1.78 (m, 2H), 2.05 (m, 2H), 2.18 (s, 3H), 2.60 (m, IH), 2.95 (m, 4H), 3.67 (m, 2H), 3.74 (s, 3H), 4.42 (m, 2H), 6.82 (m, 3H), 7.08 (s, 2H), 7.13 (s, IH), 7.29 (t, IH), 9.09 (s, IH), 10.49 (s, IH). LRMS :m/z 543 (M-l)\ Preparation 126 2-[2-(/e/-i'-butoxy)ethoxy]-6-bromopyridine Sodium hydride (6.8g, 60% dispersion in mineral oil, 0.169mol) was added portionwise to an ice-cold solution of 2-(tert-butoxy)ethanol (20. Og, 0.169mol) in toluene (500ml) under nifrogen, and the solution stined for 30 minutes whilst warming to ambient temperature. 2,6-Dibromopyridine (40.0, 0.169mol) was added, and the reaction heated under reflux for 3 hours. The mixture was allowed to cool to ambient temperature and was diluted with water (1000ml), and exfracted with ethyl acetate (2x400ml). The combined organic extracts were dried (Na 2 S0 4 ), filtered and evaporated in vacuo to give the title compound as a yellow oil (quantitative). Η nmr (CDC1 3 , 400MHz) δ: 1.21 (s, 9H), 3.67 (t, 2H), 4.40 (t, 2H), 6.68 (d, IH), 7.05 (d, IH), 7.38 (t, IH). LRMS :m/z 296/298 (M+23) + . Preparation 127 2-[2-(rert-butoxy)ethoxy]-6-(tributylstannyl)pyridine n-Butyllithium (71ml, 2.5M solution in hexanes, 0.177mol) was added dropwise to a cooled (-78°C) solution of the bromide from preparation 126 (46.3g, 0.169mol) in anhydrous THF (1000ml) under nitrogen, so as to maintain the internal temperature <-70°C, and the solution stined for 10 minutes. Tri- n-butyltin chloride (48ml, 0.177mol) was added slowly to maintain the internal temperature <-70°C, and the reaction was then allowed to warm to room temperature over 1 hour. The reaction was diluted with water (1000ml), the mixture extiacted with Et 2 0 (2x1000ml), and the combined organic extracts dried (Na 2 S0 4 ), filtered and evaporated in vacuo. The residue was purified by column chromatography on silica gel using pentane:Et 2 0 (100:1 to 98:2) as eluant, to afford the title compound as a colourless oil, (45.5g, 55%). 'H nmr (CDC1 3 , 400MHz) δ: 0.86 (t, 9H), 1.04 (m, 611), 1.21 (s, 9H), 1.35 (m, 6H), 1.58 (m, 6H), 3.69 (t, 2H), 4.43 (t, 2H), 6.58 (d, I H), 6.97 (m, IH), 7.37 (m, IH). LRMS :m/z 506/508 (M+23) + . Preparation 128 2-bromo-6-ethoxypyridine Br N O ^ Sodium ethoxide (1.5g, 63mmol sodium, in ethanol (30ml)) was added to 2,6-dibromopyridine (15g, 63mmol) in toluene (150ml) at ambient temperature under nitrogen, and the reaction heated under reflux for 5 hours. The cooled mixture was diluted with water (100ml), and exfracted with ethyl acetate (2x100ml). The combined organic extracts were dried (Na 2 SO„), filtered and evaporated in vacuo. The residue was purified by column chromatography on silica gel using pentane/ethyl acetate (100:0 to 95:5) as eluant to give the title compound as a yellow oil, (quantitative). 'H nmr (CDC1 3 , 400MHz) δ: 1.37 (t, 3H), 4.35 (q, 2H), 6.62 (d, IH), 7.01 (d, IH), 7.38 (t, IH). LRMS :m/z 202/204 (M+l) + . Preparation 129 2-ethoxy-6-(fributylstannyl)pyridine The title compound was prepared from the bromide from preparation 128 in a similar procedure to that described in preparation 127, and was isolated as a colourless oil (1.3g, 6%). Η nmr (CDC1 3 , 400MHz) δ: 0.86 (t, 9H), 1.04 (m, 6H), 1.36 (m, 9H), 1.57 (m, 6H), 4.38 (q, 2H), 6.52 (d, IH), 6.95 (m, IH), 7.37 (m, IH). LRMS :m/z 434/436 (M+23) + . Preparation 130 Methyl 4- { [4-(4-bromo-3-methylphenyl)-4-hydroxy- 1 -pipeπdm- 1 -yl]sulfonyl} tefrahydro-2N-pyran-4- carboxylate -tso-propylbromide (20ml, 0 21 mol) was added dropwise over lh to a stined mixture of magnesium (4 7g, 0 19mol) m THF (50ml) and toluene (50ml), under nifrogen The mixture was stured at room temperature for 1 hour and then cooled to 0°C A solution of 2-bromo-5-ιodotoluene (57g, 0 19mol) m toluene (50ml) was added dropwise over 30 min, between 0 and 5°C, and the mixture was stirred at 0°C for 30mm The mixture was then added dropwise over 45 mm to a stined suspension the ketone from preparation 16 (50g, 0 16mol) m toluene (250ml), between 0 and 5°C, under nitrogen The resulting mixture was stined at 0°C for 1 hour and then citnc acid solution (10%, 400ml) and ethyl acetate (200ml) were added The organic phase was separated and the aqueous phase was re-extracted with ethyl acetate (2x200ml) The combmed organic phases were washed with water (200ml) and concentiated in vacuo to a solid which was purified by re-crystallisation from toluene (500ml) to give the title compound as a colourless solid (66g, 84%) 'H nmr (CDC1 3 , 300MHz) δ 1 70-1 77 (m, 2H), 2 02-2 26 (m, 4H), 2 38-2 42 (m, 5H), 3 30 (t, 2H), 3 45 (t, 2H), 3 67-3 75 (m, 2H), 3 88 (s, 3H), 3 99 (dd, 2H), 7 14 (dd, IH), 7 31 (d, IH), 7 50 (d, IH) Preparation 131 Methyl 4-{[4-(4-{6-[2-(/ert-butoxy)ethoxy]pyndm-2-yl}-3-methylphenyl)pιpendm-l- yl]sulfonyl}tetiahydro-2N-ρyran-4-carboxylate A solution of n-butyllithium in hexanes (2 5M, 3 1ml, 7 7mmol) was added dropwise over 5 mm to a solution of the bromopyndine from preparation 126 (2 Og, 7 3mmol) in THF (20ml) at -78°C, under nitrogen The mixture was stined at -78°C for 10 min and then tπ-fso-propylborate (1.9ml, 8 Ommol) was added dropwise over 10 mm The mixture was stined at -78 C C for 10 min and then allowed to warm to room temperature over 1 hour The aryl bromide from preparation 27 (2.7g, 5.8mmol), palladium acetate (82mg, 0 36mmol), tπphenylphosphine (191mg, 0 73mmol), ethanol (20ml) and aqueous sodium carbonate (2M, 20ml) were added and the mixture was heated to reflux for 4 hours, under nitrogen, and then cooled Ethyl acetate (50ml) and demineralised water (50ml) were added and the organic phase was separated The aqueous phase was re-extracted with ethyl acetate (2x30ml) and the combined organic phases were washed with demineralised water (50ml) and then concentrated in vacuo to a solid Punfication by re-crystallisation from methanol (30ml) gave the title compound as a colourless solid (2 0g, 60%) Η nmr (CD 3 OD, 300MHz) δ. 1 12 (s, 9H), 1 50-1.69 (m, 2H), 1 72-1 88 (m, 2H), 1 91-2.05, (m, 2H), 2.24-2 30 (m, 2H), 2 34 (m, 3H), 2.65-2 78 (m, IH), 3.00-3 23 (m, 4H), 3.61 (t, 2H), 3 70-3.78 (m, 2H), 3.80 (s, 3H), 3.87-3.95 (m, 2H), 4 30 (t, 2H), 6.74 (d, IH), 7 05 (d, IH), 7.10-7.17 (m, 2H), 7.33 (d, IH), 7.73 (t, lH) LCMS :m z 575 (M+H) + Preparation 132 4-{t4-(4-{6-[2-tert-butoxyethoxy]pyndm-2-yl}-3-methylphenyl)pιpendm-l-yl]sulfonyl}-tetiahydro-2H- pyran-4-carboxyhc acid A mixture of the methyl ester from preparation 131 (9.1g, 16. Ommol) and aqueous sodium hydroxide (80ml, IN, 80.0mmol) in dioxan (250ml) were heated under reflux for 2 hours. Methanol (100ml) and aqueous sodium hydroxide (40ml, IN, 40.0mmol) were added and the mixture refluxed for a further 2 hours, then allowed to cool to ambient temperature. The reaction was concentiated m vacuo, the residue dissolved m water (200ml), and the solution acidified to pH 4 with glacial acetic acid The aqueous layer was exfracted with ethyl acetate (2x 200ml) and the combmed organic extracts were washed with bnne (200ml), men water (2x200ml), dned (Na 2 S0 4 ), filtered and concentrated m vacuo. The resultmg oily solid was azeotroped with toluene then tnturated with cold di-isopropyl ether to afford the title compound as a pale yellow solid (7.66g, 85%) 'H nnuJDMSO-d,-, 400MHz) δ: 1.13 (s, 9H), 1.61 (m, 2H), 1.79 (m, 2H), 1.95 (m, 2H), 2.22 (d, 2H), 2.32 (s, 3H), 2.66 (m, IH), 3.05 (t, 2H), 3.20 (t, 2H), 3.60 (t, 2H), 3.76 (d, 2H), 3.88 (m, 2H), 4.28 (t, 2H), 6.73 (d, IH), 7.03 (d, IH), 7.12 (m, 2H), 7.31 (d, IH), 7.75 (t, IH), 13.77 (s, IH). LRMS :m/z 583 (M+23) + . Preparation 133 NHydroxy-4-[(4-{4-[6-(2-fcrt-butoxyethoxy)pyridin-2-yl]-3-methylphenyl}piperidin-l- yl)sulfonyl]tetiahydro-2N-pyran-4-carboxamide l-(3-Dimethylaminopropyl)-3-ethylcarbodiirnide hydrochloride (3.15g, lό.Ommol) and l-hydroxy-7- azabenzotriazole (2.05g, 15.0mmol) were added to a solution of the acid from preparation 132 (7.66g, 14mmol) in anhydrous dichloromethane (80ml) and pyridine (80ml) and the reaction was stined under nifrogen for lhour. Hydroxylamine hydrochloride (2.85g, 41. Ommol) was then added, and the reaction stined at room temperature ovemight. The reaction was diluted with dichloromethane (200ml) and washed with pH 7 phosphate buffer solution (200ml). The aqueous layer was extracted with dichloromethane (2x 200ml) and the combined organic extracts were washed with dilute aqueous acetic acid (150ml), brine (150ml), then water (150ml), dried (Na 2 S0 4 ), filtered and concenfrated in vacuo. The resulting solid was azeofroped with toluene and then recrystallised from ethyl acetate and di-isopropyl ether to afford the title compound as a white solid (6.3g, 75%). 'H nmr (DMSO-de, 400MHz) δ: 1.13 (s, 9H), 1.61 (m, 2H), 1.78 (m, 2H), 1.91 (m, 2H), 2.37 (m, 5H), 2.62 (m, IH), 3.05 (t, 2H), 3.20 (t, 2H), 3.60 (t, 2H), 3.73 (d, 2H), 3.83 (m, 2H), 4.28 (t, 2H), 6.73 (d, IH), 7.03 (d, IH), 7.12 (m, 2H), 7.31 (d, IH), 7.72 (t, IH), 9.05 (s, IH), 10.90 (s, IH). LRMS :m/z 598 (M+23) + .

Claims: CLAIMS 1 N-Hydroxy 4- {[4-(4- {6-[2-hydroxyethoxy]pyndιn-2-yl}-3-methylphenyl)pιpeπdιn-l- yl]sulphonyl}tetrahydro-2H-pyran-4-carboxamιde and the pharmaceutically acceptable salts thereof, and solvates tiiereof 2 A compound selected from N-hydroxy 2-[(4-{4-[6-(2-hydroxyethoxy)pyπdιn-2-yl]-3-methylphenyl}pιpeπdιn-l-yl)sulphonyl]-2- methylpropanamide, N-hydroxy 2- {[4-(4- {6-f 2-(methoxy)ethoxy]pyπdιn-2-yl} -3-methylphenyl)pιpendιn- 1 -yl]sulphonyl} -2- methylpropanamide, N-hydroxy 4- { [4-(4- {6-[2-hydroxyethoxy]pynd -2-yl} -3-methylphenyl)pιpeπdm- 1 - yl]sulphonyl}tetrahydro-2H-pyran-4-carboxamide, N-hydroxy 4- {[4-(4-{6-[(2S)-2,3-dιhydroxy-l-propoxy]pyπdm-2-yl}-3-methylρhenyl)pιpeπdm-l- yl]sulρhonyl}tefrahydro-2H-pyran-4-carboxamιde, N-hydroxy 4- { [4-(4- {6-[(2R)-2,3-dιhydroxy- 1 -propoxy]pyπdm-2-yl} -3-methylphenyl)pιpendm- 1 - yl]sulphonyl}tefrahydro-2H-pyran-4-carboxamide, N-hydroxy 4- { [4-(4- {6-[2-hydroxyethoxy]pyπd -2-yl} -3-methylphenyl)pιpeπdm- 1 -yl]sulphonyl} - pιpeπdme-4-carboxamιde dihydrochlonde, N-hydroxy 4- { [4-(4- {6-[2-hydroxyethoxy]pyndm-2-yl} -3-methylphenyl)pιpendιn- 1 -yl]sulphonyl} - 1 - methyl-pιpeπdιne-4-carboxamιde, N-hydroxy 2-[4-(4- (3-[(2S)-2,3-dιhydroxy- 1 -propoxy]phenyl} -3-methylphenyl)-pιpendm- 1 - ylsulphonyl]-2-methylpropanamιde, N-hydroxy 4- {4-[4-(3-[(2R)-2,3-dιhydroxy- 1 -propoxy]phenyl)-3-methylphenyl]-pιpendm- 1 - ylsulphonyl} -tetrahydro-(2H)-pyran-4-carboxamιde, N-hydroxy 4- {4-[4-(3- {(2S)-2-hydroxy-2-hydroxymethyl} ethoxyphenyl)-3-methylphenyl]-pιpendm- 1 - ylsulphonyl}-tetiahydro-2H-pyran-4-carboxamide, N-hydroxy 4- {4-[4-(3-{l,3-dιhydroxy-2-propoxyphenyl)-3-methylphenyl]-pιpendm-l -ylsulphonyl} - tetrahydro-2H-pyran-4-carboxamιde, N-hydroxy 2- { [4-(4- {3- [2-(methylammo)ethoxy]phenyl } -3 -methylphenyl)-pιpendιn- 1 -y I] sulphonyl } -2- methylpropanamide hydrochloπde, N-hydroxy 2-[4-(4- {3-(2-ammoethoxy)phenyl} -3-methylphenyl)-pιpeπdιn- 1 -ylsulphonyl] -2- methylpropanamide hydrochlonde, N-hydroxy 4- {[4-(-4- {6-[2-ammoethoxy]pyndm-2-yl} -3-methylphenyl)pιpendm- 1 - yl]sulphonyl}tefrahydro-2H-pyran-4-carboxamιde hydrochlonde, N-hydroxy 2-[4-(4-{3-(2-N,N-dιmethylaπunoethoxy)phenyl}-3-methylphenyl)-pιpeπdm-l-ylsulphonyl]- 2-methylpropanamιde, N-hydroxy 4- {[4-(4-{3-(methyl)ar--unomethyl}-3-methylphenyl)pιpeπdm-l-yI]sulphonyl}tetrahydro-2H- pyran-4-carboxamιde hydrochloπde, N-hydroxy 4- {[4-(3-methyl-4- {3-[4-mo holιnylmethyI]}phenyl)pιpeπdιn-l-yl]sulphonyl}tetiahydro- 2H-pyran-4-carboxamιde, N-hydroxy 2-({4-[4-(3-methoxy- IH-pyrazol- l-yl)-3-methylphenyl]pιpeπdιn- l-yl}sulphonyl)-2- methylpropanamide, N-hydroxy 2-[(4- {4-[3-(2-hydroxyethoxy)-lH-pyrazol-l-yl]-3-methylphenyl}pιpeπdιn-l-yl)sulphonyl]- 2-methylpropanamιde, N-hydroxy 2-methyl-2-( {4-[3-methyl-4-( 1 ,3-thιazol-2-yl)phenyl]pιpeπdιn- 1 -yl} sulphonyl)propanaπude, (lα,3α,4α)-N3,4-tnhydroxy-l-[(4-{4-[6-(2-hydroxyethoxy)pyπdιn-2-yl]-3-methylphenyl}pιpeπdιn-l- yl)sulfonyl]cyclopentanecarboxamιde, (1 α,3α,4α)- 1 -( {4-[4-(6-ethoxypyndιn-2-yl)-3-methylphenyl]pιpendm- 1-yl} sulfonyl)-/V,3,4- tπhydroxycyclopentanecarboxamide, ( 1 α,3 β,4β)- 1-( {4-[4-(6-ethoxypyndιn-2-yl)-3-methylphenyl]pιpeπdιn-l -yl} sulfonyl)-N3,4- tnhydroxycyclopentanecarboxamide, ( 1 α,3α,4α)-N3 ,4-tπhydroxy- 1 - {4-[4-(3-methoxyphenyl)-3-methylphenyl]pιpeπdιn- 1 - ylsulfonyl}cyclopentanecarboxamιde; and ( 1 α,3 β,4 β)-N,3 ,4-tπhydroxy- 1 - {4-[4-(3-methoxyphenyl)-3-methylphenyl]pιpendιn- 1- ylsulfonyl } cyclopentanecarboxamide, and the pharmaceutically acceptable salts thereof, and solvates thereof 3 Ν-Hydroxy 4-{[4-(4-{6-[2-hydroxyethoxy]pyπdm-2-yl}-3-methylphenyl)pιpeπdm-l-yl]sulphonyl}- pιpendme-4-carboxamιde and the pharmaceutically acceptable salts thereof, and solvates thereof 4. Ν-Hydroxy 4- {[4-(-4- {6-[2-amιnoethoxy]pyndιn-2-yl} -3-methylphenyl)pιpendm- 1 - yl]sulphonyl}tetrahydro-2H-pyran-4-carboxamιde and the pharmaceutically acceptable salts thereof, and solvates thereof. 5. A compound of formula (I): (I) and pharmaceutically-acceptable salts thereof, and solvates thereof, wherem the dotted lme represents an optional bond, X is a monocyclic aromatic linker moiety selected from pyrazolylene, thiazolylene, pyrazinylene, pyridazinylene, pynolylene, oxazolylene, isoxazolylene, oxadiazolylene, thiadiazolylene, imidazolylene, triazolylene, or tetrazolylene, R is H, C alkyl optionally substituted by C alkoxy or NR 4 R 5 or OH, or C,^ alkoxy optionally substituted by 1 or 2 substituents selected from (C alkyl optionally substituted by OH), C alkoxy, OH and NR 4 R 5 , R 1 and R 2 are each mdependently H, C, 6 alkyl optionally substituted by OH or C alkoxy, or C^ alkenyl, or R 1 and R 2 are taken, together with the C atom to which they are attached, to form a 3- to 7-membered rmg optionally incorporatmg a hetero- moiety selected from O, S, SO, S0 2 and NR 6 , and which 3- to 7- membered nng is optionally substituted by one or more OH, R 3 is H, halo, metiiyl, or methoxy, R 4 and R 5 are each mdependently H or C, to C 6 alkyl optionally substituted by OH, C, to C 4 alkoxy or aryl, or R 4 and R 5 can be taken together with the N atom to which they are attached , to form a 3- to 7- membered rmg, optionally incorporating a further hetero- moiety selected from O, S, S0 2 and NR 7 ; and R 6 and R 7 are each mdependently H or C, to C 4 alkyl 6 A compound of formula (I) (I) and pharmaceutically-acceptable salts thereof, and solvates thereof, wherem the dotted lme represents an optional bond; X is a monocyclic aromatic linker moiety selected from phenylene, pyndinylene, pyrazolylene, thiazolylene, thienylene, furylene, pynmidmylene, pyrazinylene, pyπdazinylene, pyrrolylene, oxazolylene, isoxazolylene, oxadiazolylene, thiadiazolylene, imidazolylene, tnazolylene, or tetrazolylene, R is C alkyl substituted by NR R 5 , C,^ alkoxy substituted by NR R 5 , or C alkoxy substituted by 2 substituents selected from (C alkyl optionally substituted by OH), C M alkoxy, OH and NR 4 R 5 , R 1 and R 2 are each independently H, C w alkyl optionally substituted by OH or C alkoxy, or C 2 ^ alkenyl, or R 1 and R 2 are taken together, with the C atom to which they are attached, to form a 3- to 7-membered ring optionally incorporating a hetero- moiety selected from O, S, SO, S0 2 and NR 6 , and which 3- to 7- membered πng is optionally substituted by one or more OH, R 3 is H, halo, methyl, or methoxy, R 4 and R 5 are each independently H or C, to C 6 alkyl optionally substituted by OH, C, to C„ alkoxy or aryl, or R 4 and R 5 can be taken together with the N atom to which they are attached , to form a 3- to 7- membered πng, optionally mcorporatmg a further hetero- moiety selected from O, S, S0 2 and NR 7 , and R 6 and R 7 are each independently H or C, to C 4 alkyl 7 A compound of formula (I) (I) and pharmaceutically-acceptable salts thereof, and solvates thereof, wherem the dotted lme represents an optional bond, X is a monocyclic aromatic linker moiety selected from phenylene, pyndmylene, pyrazolylene, thiazolylene, thienylene, furylene, pynmidmylene, pyrazinylene, pyndazinylene, pynolylene, oxazolylene, isoxazolylene, oxadiazolylene, thiadiazolylene, imidazolylene, tπazolylene, or tetrazolylene, R is H, C,- 4 alkyl optionally substituted by C alkoxy, NR R 5 or OH, or C M alkoxy optionally substituted by 1 or 2 substituents selected from (C alkyl optionally substituted by OH), C alkoxy, OH and NR 4 R 5 , R' and R 2 are each mdependently C,^ alkyl substituted by OH, or R 1 and R 2 are taken together, with the C atom to which they are attached, to form a 3- to 7-membered rmg optionally mcorporatmg a hetero- moiety selected from O, S, SO, S0 2 and NR 6 , and which 3- to 7- membered πng is substituted by one or more OH, R 3 is H, halo, methyl, or methoxy, R 4 and R 5 are each mdependently H or C, to C 6 alkyl optionally substituted by OH, C, to C 4 alkoxy or aryl, or R 4 and R 5 can be taken together with the N atom to which they are attached , to form a 3- to 7- membered πng, optionally mcorporatmg a further hetero- moiety selected from O, S, S0 2 and NR 7 , and R 6 and R 7 are each mdependently H or C, to C 4 alkyl 8 A compound, salt or solvate according to claim 6 or claim 7 where X is phenylene, pyπdinylene, pyrazolylene or thiazolylene 9 A compound, salt or solvate according to claim 8 wherem X is 1,3-phenylene, 2,6-pyndmylene, 1,3- pyrazolylene or 2,5-thiazolylene 10 A compound, salt or solvate according to claim 5 wherem X is pyrazolylene or thiazolylene 11 A compound, salt or solvate accordmg to claim 10 wherem X is 1,3-pyrazolylene or 2,5-thiazolylene 12 A compound, salt or solvate accordmg to claim 5 or claim 7 wherein R is H, methoxy, 0(CH 2 ) 2 OH, 0(CH 2 ) 2 OCH 3 , 0(CH 2 ) 2 N(CH 3 ) 2 , 0(CH 2 ) 2 NHCH 3> 0(CH 2 ) 2 NH 2 , CH 2 NHCH 3 , morpholinomethyl, 2- morphohnoethoxy, 2R-2,3-dιhydroxy-l-propyloxy, 2S-2,3-dιhydroxy-l-propyloxy or l,3-dιhydroxy-2- propyloxy. 13 A compound, salt or solvate accordmg to claim 12 wherem R is 0(CH 2 ) 2 OH or 0(CH 2 ) 2 NH 2 . 14. A compound, salt or solvate accordmg to claim 6 wherein R is 0(CH 2 ) 2 N(CH 3 ) 2 , 0(CH 2 ) 2 NHCH 3 , 0(CH 2 ) 2 NH 2 , CH 2 NHCH 3 , moφholmomethyl, 2-moφholmoethoxy, 2R-2,3-dιhydroxy-l -propyloxy, 2S- 2,3 -dιhydroxy-1 -propyloxy or 1 ,3-dιhydroxy-2-propyloxy. 15. A compound, salt or solvate accordmg to claim 14 wherem R is 0(CH 2 ) 2 NH 2 . 16. . A compound, salt or solvate accordmg to claim 5 or claim 6 wherem R 1 and R 2 are each mdependently C,^ alkyl optionally substituted by OH, or R' and R 2 are taken together, with the C atom to which they are attached, to form a 3- to 7-membered πng optionally incoφorating a hetero- moiety selected from O, S, SO, S0 2 and NR 6 , and which 3- to 7- membered rmg is optionally substituted by one or more OH. 17. A compound, salt or solvate accordmg to claim 16 wherem R 1 and R 2 are each CH 3 , or R 1 and R 2 are taken together, with the C atom to which they are attached, to form a tefrahydropyran-4- ylidene, pιpendm-4-ylιdene, l-methylpιpendιn-4-ylιdene, or 3,4-dιhydroxycyclopentylιdene moiety. 18. A compound, salt or solvate accordmg to claim 17 wherem R 1 and R 2 are taken together, with the C atom to which they are attached, to form a tetrahydropyran-4-yhdene, cι-ϊ-3,4- dihydroxycyclopentyhdene, /rαn-s-3,4-dιhydroxycyclopentyhdene or pιpendιn-4-yhdene moiety. 19. A compound, salt or solvate according to claim 18 wherem R 1 and R 2 are taken together, with the C atom to which they are attached, to form a tetiahydropyran-4-ylιdene, pιpeπdιn-4-yhdene, or cιs-3,4- dihydroxycyclopentylidene where the hydroxy substituents have a -relationship to the hydroxamate moiety 20 A compound, salt or solvate according to claim 7 wherein R 1 and R 2 are taken together, with the C atom to which they are attached, to form a 3, 4-dιhydroxy cyclopentylidene moiety 21 A compound, salt or solvate according to claim 20 wherein R 1 and R 2 are taken together, with the C atom to which they are attached, to form a c;.s-3,4-dιhydroxycyclopentylιdene group where the hydroxy substituents have a -relatιonshιp to the hydroxamate moiety 22 A compound, salt or solvate according to any one of claims 5 to 21 wherein R 3 is methyl and the optional double bond depicted as a dotted line in formula (I) is absent 23 A pharmaceutical composition comprising a substance according to any one of claims 1 to 22 and a pharmaceutically acceptable diluent, adjuvant or earner 24 A substance according to any one of claims 1 to 22 for use as a medicament 25 The use of a substance according to any one of claims 1 to 22 m the manufacture of a medicament for the treatment of a MMP-mediated disease, condition or process 26 A method of treatment of a MMP-mediated disease, condition or process comprising administration of an effective amount of a substance according to any one of claim 1 to 22 27 A compound selected from methyl 4-(4-oxo-pιpeπdιn- 1 -ylsulphonyl)tefrahydro-2H-pyran-4-carboxylate, methyl 4- {[4-(4-bromo-3-methylphenyl)-4-hydroxy-l-pιpeπdιn-l-yl]sulfonyl}tefrahydro-2/ -pyran-4-carboxylate, methyl 4- {[4-(4-{6-[2-(to ?-butoxy)ethoxy]pyπdm-2-yl}-3-methylphenyl)pιpeπdιn-l-yl]sulfonyl}tetrahydro-2/ - pyran-4-carboxylate , 4-{[4-(4-{6-[2-tert-butoxyethoxy]pyndm-2-yl}-3-methylphenyl)pιpeπdιn-l-yl]sulfonyl}-tetrahydro-2H-pyran-4- carboxylic acid, and /V-hydroxy-4-[(4- {4-[6-(2-/e; r-butoxyethoxy)pyπdιn-2-yl]-3-methylphenyl}ριpeπdιn- 1 -yl)sulfonyl]tefrahydro-2N- pyran-4-carboxamιde 28 A compound selected from 5 N-hydroxy l-(tert-butoxycarbonyl)-4-{[4-(4-{6-[2-hydroxyethoxy]pyπdιn-2-yl}-3-methylphenyl)pιpeπdm-l- yl]sulphonyl}-pιpeπdιne-4-carboxamιde, l-(tert-butoxycarbonyl)- 4-[4-(4-{6-[2-hydroxyethoxy]pyndm-2-yl}-3-methylphenyl)pιρeπdm-l-ylsulphonyl]- pιpendme-4-carboxylιc acid, methyl l-(tert-butoxycarbonyl)- 4- {[4-(4- {6-[2-hydroxyethoxy]pyπdιn-2-yl}-3-methylphenyl)ριpeπdιn- l-yl]sulphonyl}-4-pιpeπdιnecarboxylate, methyl 4- {[4-(4- {6-[2-hydroxyethoxy]pyπdιn-2-yl}-3-methylphenyl)pιpendm-l-yl]sulphonyl}- pιρeπdme-4-carboxylate, methyl 1 -benzyl-4- { [4-(4- { 6-[2-benzyloxyethoxy]pyπdιn-2-yl} -3 -methylphenyl)pιpeπdm- 1 - yl]sulphonyl}-pιpeπdιn-4-carboxylate, methyl 1 -benzyl-4- [4-(4-bromo-3-methylphenyl)pιpeπdιn- 1 -ylsulphonyl]~4-pιpeπdιnecarboxylate, and methyl 2-[4-(4-bromo-3-methylphenyl)pιpendιn- 1 -ylsulphonyl] acetate 29 A compound selected from N-hydroxy 4-[4-(4- {3-(2-[(N-tert-butoxycarbonyl)amιno]ethoxy)phenyl} -3-methylphenyl)-pιpeπdιn- 1 - ylsulphonyl]-tetrahydro-2H-pyran-4-carboxamιde, Preparation 84, methyl 4-[4-(4-{3-(2-[(tert-butoxycarbonyl)amιno]ethoxy)phenyl}-3-methylphenyl)-pιpeπdm-l- ylsulphonyl]-tettahydro-2H-pyran-4-carboxylate, methyl 4-[4-(4-{3-(2-aπunoethoxy)phenyl}-3-methylphenyl)-pιpendιn-l-ylsulphonyl]-tetrahycuO-2H- pyran-4-carboxylate, Preparation 61, methyl 4-[4-(4- {3-(2-oxoethoxy)phenyl} -3-methylphenyl)-pιpendm- 1 -ylsulphonyl]-tetrahydro-2H- pyran-4-carboxylate, and methyl 4-[4-(4- {3-(2,2-dιethoxyethoxy)phenyl} -3-methylρhenyl)-pιpendιn- 1 -ylsulphonyl]-tefrahydro- 2H-pyran-4-carboxylate 30 A compound selected from 4-[4-(4-{6-[2-hydroxyethoxy]pyndm-2-yl}-3-methylphenyl)ριpeπdm-l-ylsulphonyl]tefrahydro-2H- pyran-4-carboxylιc acid, methyl 4-{[4-(4-{6-[2-hydroxyethoxy]pyndm-2-yl}-3-methylphenyl)pιpendm-l- yl]sulphonyl}tetrahydro-2H-pyran-4-carboxylate, methyl 4-[4-(4-{6-[2-benzyloxy]ethoxypyπdm-2-yl}-3-methylphenyl)-l,2,3,6-tetrahydiOpyπdm-l- ylsulphonyl]tetrahydro-2H-pyran-4-carboxylate, and methyl 4-[4-(4-bromo-3-methylphenyl)-l,2,3,6-tetrahydropyndm-l-ylsulphonyl]tetrahydiO-2H-pyran-4- carboxylate 31 A compound of formula (VI) (VI) wherein the substituents R 1 , R 2 , R 3 , X and R are as defined above in relation to any one of claims 5, 6 or 7. 32. A compound of formula (VII): (VII) wherein R 1 , R 2 , R 3 and X are as defined in any one of claims 5, 6 or 7, and where R p is a NH- and/or OH- protected version of the conesponding compound of formula (I) as defined in any one of claims 5, 6 or 7, and where the conesponding compound of formula (I) as defined in any one of claims 5, 6 or 7 contains a free NH, NH 2 or OH group. 33. A process for making a compound of formula (I) as defined in any one of claims 5, 6 or 7 where R contains a free NH, NH 2 or OH group, which comprises deprotecting a conesponding compound of formula (VII) as defined in claim 32. 34. A compound of formula (VIII) or (IX): (VIII) (IX) where R 3 , X and R are as defined in any one of claims 5, 6 or 7. 35. A compound of formula (X) or (XI) : (X) (XI) wherein R 3 , X and R are as defined in any one of claims 5, 6 or 7, R p is as defined in claim 32, and P and P 1 are OH-protecting groups which may be taken independently or together. 36. A compound of formula (XII): (XII) wherein R 3 , X and R are as defined in any one of claims 5, 6 or 7 and R lp and R p is a N- and/or O- protected precursor which, on deprotection would give a conesponding compound of formula (I) as defined in the conesponding claim 5, 6 or 7. 37. A process for making a compound of formula (I) as defined in any one of claims 5, 6 or 7 where R 1 and or R 2 contains a free NH, NH 2 or OH group, which comprises deprotecting a conesponding compound of formula (XII) as defined in claim 36. 38. A compound of formula (II) : («l) where R 1 , R 2 , R 3 , X and R are as defined in any one of claims 5, 6 or 7, and where Z is a leaving group such as chloro, bromo, iodo, C,. 3 alkyloxy or HO. 39. A process for making a compound of formula (I) as defined in any one of claims 5, 6 or 7, which comprises reaction of a compound of formula (II) as defined in claim 38 with hydroxylamine. 40. A compound of formula (XIII): (XIII) wherein R\ X and R are as defined in any one of claims 5, 6 or 7 and R lp , R 2p and R are independently a N- and or O-protected precursor which, on deprotection would give a conesponding compound of formula (I) as defined in the conesponding claims 5, 6 or 7 where R 1 , R 2 and R contain a free NH, NH 2 and/or OH group.

Inventors: DACK KEVIN NEIL, FRAY MICHAEL JONATHAN, LEWIS MARK LLEWELLYN, THOMSON NICHOLAS MURRAY, WHITLOCK GAVIN ALISTAIR, DACK, KEVIN, NEIL, FRAY, MICHAEL, JONATHAN, LEWIS, MARK, LLEWELLYN, THOMSON, NICHOLAS, MURRAY, WHITLOCK, GAVIN, ALISTAIR
Applicants: DACK KEVIN NEIL, FRAY MICHAEL JONATHAN, LEWIS MARK LLEWELLYN, PFIZER, PFIZER LTD, THOMSON NICHOLAS MURRAY, WHITLOCK GAVIN ALISTAIR, DACK, KEVIN, NEIL, FRAY, MICHAEL, JONATHAN, LEWIS, MARK, LLEWELLYN, PFIZER INC., PFIZER LIMITED, THOMSON, NICHOLAS, MURRAY, WHITLOCK, GAVIN, ALISTAIR
Application year: 2000
Main classification: A61K31
IPC codes: A61K31, A61P1, A61P7, A61P9, A61P19, A61P27, A61P29, A61P35, A61P43, C07D211, C07D401, C07D405, C07D417