DNA METHYLTRANSFERASE INHIBITORS

Abstract: This invention provides broad-spectrum antibiotics that are inhibitors of bacterial adenine DNA methyltransferases.

Description: DNA METHYL TRANSFERASE INHIBITORS BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to the field of antibiotics and particularly antibacteπal compounds. The invention specifically relates to antibiotics targeted to DNA modification enzymes, in particular adenme DNA methyltransferases, that are the components of a broad variety of different bacterial pathogens including those that are essential for bactenal cell growth. The invention particularly provides inhibitors of such adenme DNA methyltransferases having little or no inhibitory effects on cytosme methyltransferases, and hence having limited antibiotic effect on eukaryotic, particularly mammalian, cells. Methods for preparing and using the adenme DNA methyltransferase inhibitors of the invention, and pharmaceutical compositions thereof, are also provided. 2. Background of the Invention One hallmark of the modern era of medicine has been the decline in morbidity and mortality associated with bactenal infections The development of a variety of antibiotic drugs in the early and middle parts of the twentieth century provided medical practitioners for the first time with effective treatments for a variety of infectious diseases. However, misuse of conventional antibiotics and natural selection of the infectious bactenal population has resulted in the development of varying degrees of drug resistance by most bactenal infectious agents to most antibiotic agents. In severe cases, such as MRSA (Multidrug-Resistant StaphA), one or only a few antibiotics are currently effective. In addition, the existence of immunodeficiency syndromes results in additional incidence of opportunistic infections requmng intensive antibiotic treatment. Thus, there is an increasing need in the art for novel, more effective antibiotic compounds for treating bactenal infections that are resistant to currently available therapies. Most bacteria modify their genomic DNA by methylation of specific nucleotide bases. DNA methylation is critical to gene regulation and repair of mutational lesions (see Jost & Soluz, 1993, DNA METHYLATION, MOLECULAR BIOLOGY AND BIOLOGICAL SIGNIFICANCE, Birhauser Verlag: Basel, Switzerland; Palmer & Mannus, 1994, Gene 143 1-12, Dryden, 1999, "Bacterial DNA Methyltransferases," in S- ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASES STRUCTURES AND FUNCTIONS, X. Cheng and R. M. Blumenthal (eds ), World Scientific Publishing, p.283- 340 for review). DNA methylation is catalyzed by a class of enzymes having different sequences specificities. There are those DNA methyltransferases for example (dam) that methylate adenme residues in GATC sequences or cytosme (dcm) residues m CCAGG or CCTGG sequences which are not contained in the recognition site of a cognate restriction enzyme There are those DNA methyltransferases that methylate residues contained in the recongmtion site of a cognate restriction enzyme (for example, Apal, Avafλ, Bell, Clal, Dpnll, EcoRI, Hαelll, Hhal, Mbol, and Mspl; see, Mannus & Morns, 1973, J Bactenol. 114. 1143-1150; May & Ηatman, 1975, J Bacterial 123. 768-770, Ηeitman, 1993, Genet. Eng. 1_5: 57-108) In addition, the instant inventors have discovered an adenme DNA methyltransferase from Caulobacter cresentus that methylates the adenme residue in the sequence GANTC, as disclosed m International Application Publication No. WO98/12206. This methyltransferase is cell-cycle regulated and essential for successful bactenal cell growth; inhibition of the enzyme makes the bacteria non-viable. Similar methyltransferases have also been discovered m Brucella abortus, Hehcobacter pylori, Agrobacterium tumefaciens and Rhizobium mehloti. In contrast with bacterial cells, DNA methylation in eukaryotic, and particularly mammalian cells, is limited to cytosme methylation at sites comprising the sequence CpG (Razin & Riggs, 1980, Science 210: 604-610; Jost & Bruhat, 1997, Prog Nucleic Acid Res. Molec Biol 57: 217-248). Thus, the existence of DNA methylation, in particular, the cell-cycle regulated adenme DNA methyltransferase found by the inventors m certam bactenal species, addresses the need in the art for novel targets for antibiotic activity SUMMARY OF THE INVENTION The invention provides antibiotic compounds capable of inhibiting adenme DNA methyltransferases m bacterial cells. The antibiotic compounds of the invention specifically inhibit adenme-specific bacterial DNA methyltransferases, and do not inhibit bacterial or eukaryotic, particularly mammalian and most particularly human, cytosme- specific DNA methyltransferases. The compounds of the invention also inhibit adenme- specific DNA methyltransferases in plants. The antibiotic compounds are also provided as pharmaceutical compositions capable of being administered to an animal, most preferably a human, for treatment of a disease having a bacterial etiology, or an opportunistic infection with a bactena m an animal, most preferably a human, in an lmmunologically compromised or debilitated state of health. The invention also provides methods for preparing the antibiotic compounds and pharmaceutical compositions thereof, and methods of using said antibiotics therapeutically. Kits and packaged embodiments of the antibiotic compounds and pharmaceutical compositions of the invention are also provided. Specific preferred embodiments of the present invention will become evident from the following more detailed description of certain prefened embodiments and the claims. DESCRIPTION OF THE DRAWINGS Figures 1 and 2 depict schematic diagrams of the "active site" of bactenal adenme DNA methyltransferases. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS This invention provides antibiotics, and specifically antibactenal compounds, that are inhibitors of bacterial adenme DNA methyltransferases. The compounds of the invention exhibit antibacterial, growth-inhibitory properties against any bactenal species that produces an adenme DNA methyltransferase. These include adenme DNA methyltransferases that are components of bactenal restriction/modification systems as understood in the art, as well as cell-cycle regulated adenme DNA methyltransferases (CcrM), such as those disclosed in International Application Publication No. WO98/12206, incorporated by reference. Thus, inhibitors of adenme DNA methyltransferases are particularly provided by the invention. The adenme DNA methyltransferase inhibitors of the invention comprise a novel class of broad-spectrum antibiotics. Most bactenal species possess a DNA methyltransferase that is part of a modification apparatus, typically associated with a restriction enzyme, that preserves the integrity of cellular DNA while providing a defense against foreign (most typically viral) DNA. In addition, certain bacteria produce an adenme DNA methyltransferase that is essential for bactenal cell growth. Medically- important bactenal species that provide appropπate targets for the antibactenal activity of the inhibitors of the invention include gram-positive bacteria, including cocci such as Staphylococcus species and Streptococcus species; bacilli, including Bacillus species, Corynebacterium species and Clostridium species, filamentous bactena, including Actinomyces species and Streptomyces species, gram-negative bacteria, including cocci such as Neissena species, bacilli, such as Pseudomonas species, Brucella species, Agrobactenum species, Bordetella species, Escherichia species, Shigella species, Yersinia species, Salmonella species, Klebsiella species, Enterobacter species, Hemophύus species, Pasteurella species, and Streptobacillus species, spirochetal species, Campylobacter species, Vibrio species; and intracellular bacteria including Rickettsiae species and Chlamydia species. Specific bacterial species that are targets for the adenme DNA methyltransferase inhibitors of the invention include Staphylococcus aureus, Staphylococcus saprophytwus; Streptococcus pyrogenes, Streptococcus agalactiae; Streptococcus pneumomae; Bacillus anthracis; Corynebacterium diphtheria, Clostridium perfringens; Clostridium botuhnum; Clostridium tetani, Neissena gonorrhoeae, Neissena meningitidis , Pseudomonas aeruginosa, Legionella pneumophila, Escherichia coh; Yersinia pestis, Hemophύus influenzae; Hehcobacter pylori, Campylobacter fetus; Vibrio cholerae, Vibrio parahemolyhcus; Trepomena palhdum; Actinomyces israelu; Rickettsia prowazeku; Rickettsia rickettsu; Chlamydia trachomatis, Chlamydia psittaci; Brucella abortus and Agrobactenum tumefaciens. It is an important property of the adenme DNA methyltransferase inhibitors of the invention that the level of activity of these substances with cytosme-specific DNA methyltransferases is low This is because cytosine-specific DNA methyltransferases occur m mammalian, most particularly human, cells, and it is an advantageous property of the adenme DNA methyltransferases of the invention to have little or no inhibitory activity against mammalian methyltransferases. This property confers upon the molecules provided by the invention the beneficial property of being bactenal cell specific, and having little antibiotic activity against mammalian, most preferably human, cells. Preferably, the IC 50 of these compounds for cytosine-specific DNA methyltransferases is greater than 500μM. The inhibitory compounds provided by the invention are represented by Formula T - A - where R 1 , R and R are the same or different and are independently hydrogen, lower alkyl, aryl or substituted aryl, lower alkoxy, lower alkoxyalkyl, or cycloalkyl or cycloalkyl alkoxy, where each cycloalkyl group has from 3-7 members, where up to two of the cycloalkyl members are optionally hetero atoms selected from oxygen and nitrogen, and where any member of the alkyl, aryl or cycloalkyl group is optionally substituted with halogen, lower alkyl or lower alkoxy, aryl or substituted aryl, and where R 3 can be ribose, deoxynbose or phosphorylated derivatives thereof, including phosphorothioates, phosphoramidites and similar derivatives known in the art, provided that R 1 , R 2 and R 3 are not all hydrogen, and where R 3 is ribose, deoxynbose or phosphorylated derivatives thereof, R 1 and R 2 are not both hydrogen. In preferred embodiments, R is H, R is (2-dιphenylbonnιc ester) ethyl or diphenylpropyl, and R is H, 2-(4-morpholmyl)-ethyl, 3-(N-phthaloyl)-ammopropyl, 2-(2-(2- hydroxyethoxy)ethoxy)ethyl, or ethyl-2-(acrylate)-methyl. In additional preferred embodiments, R 1 is H, R 2 is (S-homocystemyl)methyl and R 3 is ribose, 5'phosphorylnbose, deoxynbose or 5' phosphoryl deoxynbose. In other preferred embodiments, R 3 is H and R 1 and R 2 are together 2-(dιphenylmethyl) cyclopentyl or 2- (diphenylhydroxymethyl) cyclopentyl. In further preferred embodiments, R 1 is H, R is alanylbutyl ester, 2-carboxιmιdo-2-ammoethyl, 2-ammoethyl or mono- or bisubstituted 2-ammo ethyl, and R is 2-(4-morpholmyl)-ethyl. The invention also provides compounds of Formula IP wherein bonds 1 and 2 can be double or single, Ar 1 and Ar 2 can be the same or different and are each independently aryl or heteroaryl, or aryl or heteroaryl substituted at one or a plurality of positions with halogen, nitro, nitroso, lower alkyl, aryl or substituted aryl, lower alkoxy, lower alkoxyalkyl, or cycloalkyl or cycloalkyl alkoxy, where each cycloalkyl group has from 3-7 members, where up to two of the cycloalkyl members are optionally hetero atoms selected from sulfur, oxygen and nitrogen, and where any member of the alkyl, aryl or cycloalkyl group is optionally substituted with halogen, lower alkyl or lower alkoxy, aryl or substituted aryl, halogen, nitro, nitroso, aldehyde, carboxyhc acid, amide, ester, or sulfate, and R a , R and R c are the same or different and are independently hydrogen, halogen, nitro, nitroso, lower alkyl, lower alkoxy, lower alkoxyalkyl, or cycloalkyl or cycloalkyl alkoxy, or aryl or heteroaryl, or aryl or heteroaryl substituted at one or a plurality of positions with lower alkyl, lower alkoxy, lower alkoxyalkyl, or cycloalkyl or cycloalkyl alkoxy, where each cycloalkyl group has from 3-7 members, where up to two of the cycloalkyl members are optionally hetero atoms selected from sulfur, oxygen and nitrogen, and where any member of the alkyl, aryl or cycloalkyl group is optionally substituted with halogen, lower alkyl or lower alkoxy, aryl or substituted aryl, halogen, nitro, nitroso, aldehyde, carboxyhc acid, amide, ester, or sulfate, or wherein R a , R b and R c may be connected by aromatic, aliphatic, heteroaromatic, heteroahphatic ring structures or substituted embodiments thereof. The invention also provides combinatonal chemical libraries of punne derivatives. In one preferred embodiment, 6-chloropunne is converted into adenme derivatives by animation of the C6 position of the punne ring; these libraries are termed "N6 hbranes" herein. In other preferred embodiments, unsubstituted adenme or 6- chloropurme is denvatized at the N9 position of the punne ring; these libraries are termed "N9 libraries" herein. In still further embodiments, both the C6 and N9 positions are denvatized, with the C6 position being animated with an amme or substituted ammo group; these libraries are termed "N6/N9 hbranes" herein. In the preparation of the N6 or N9 hbranes, the starting punne ring structure is reacted in individual "pots" or reaction mixtures with each of a plurality of amines or substituted amines (for N6 hbranes) or halides (for N9 hbranes). These libraries thus are provided as collections of separate products of the reaction between the starting materials. For N6/N9 libraries, most preferably the N9 position is first denvatized followed by reaction at the C6 position. In these libraries, reaction is typically performed using a single halide (resulting m uniform substitution at the N9 position) and a plurality of amines (preferably 2 to 5 amines, most preferably 3 different amines), thereby providing a mixture of compounds. In addition, regioisomers (including the Nl , N3, and N7 isomers) can be produced according to the methods of the invention. Typically, reaction mixtures are also provided lacking the punne starting material, to monitor for reactions between the halides and the different am-mes The invention also provides so-called "rational design" adenme DNA methyltransferase inhibitors, based on an understanding of the putative active site of an adenme DNA methyltransferase enzyme, shown m Figure 1. As schematically depicted in the Figure, the enzyme has a binding site for the adenme residue in a DNA strand, and an S-adenosylmethionme binding site, which provides the donor methyl group as shown. So-called "rational design" inhibitors mimic the configuration of the molecules in the binding site of the enzyme, as shown in Figure 2. These compounds in general comprise an adenosme residue, with or without a 5' phosphate group, covalently linked through a methylene bπdge to a homocysteine moiety. The invention also provides adenme DNA methyltransferase inhibitors that are derivatives of borimc acid, most preferably diphenyl or substituted diphenyl boπnic acid, and most preferably diphenyl or substituted diphenyl borimc acid alkylamme esters thereof. In preferred embodiments, the invention provides compounds including dι-(p- fluorophenyl)bonnιc acid 8- hydroxyqumilme ester, dι-(p-chlorophenyl)bormιc acid 8- hydroxyqumilme ester, diphenylbonmc acid 8-hydroxyqumιlme ester, dι-(p- fluorophenyl)bonnιc acid ethanolamme ester, and dι-(p-chlorophenyl)bormιc acid ethanolamme ester The invention also provides adenme DNA methyltransferase inhibitors synthesized using solid phase chemistry, most preferably using resins compπsmg a residue (such as an amine or halide) as provided herein for substitution at the C6 or N9 positions of the punne nng. In preferred embodiments, these resins are provided whereby the substituent is covalently linked to the resin using a covalent bond that can be specifically cleaved to liberate the compound from the resin after solid phase synthesis is complete. Preferably, the substituent is presented on the resm with an activated group, such as an amme or halide, accessible to a punne contacted with the resm. After reaction, the punne is linked to the resm through the substituent, and the reaction product can then be worked up and removed from the resm using methods well known in the art. See, for example, Bumn, 1998, THE COMBINATORIAL INDEX, Academic Press. In certain situations, compounds of the invention may contain one or more asymmetnc carbon atoms, so that the compounds can exist in different stereoisomenc forms. These compounds can be, for example, racemates or optically active forms. In these situations, the single enantiomers, i.e., optically active forms, can be obtained by asymmetric synthesis or by resolution of the racemates. Resolution of the racemates can be accomplished, for example, by conventional methods such as crystallization in the presence of a resolving agent, or chromatography, using, for example a chiral HPLC column. Advantageously, solid phase chemistry employing resms as described above can be useful for determining whether a substituent exhibits chirahty or stereospecificity that has a beanng on antibacterial activity. In these embodiments, compounds are prepared for screening using a racemic mixture of optically-active species, such as an ammo acid. Upon finding the resulting compound has adenme DNA methyltransferase inhibitory activity, optically-pure preparations of each of the stereoisomers can be used to prepare the corresponding optically-pure isomers of the adenme DNA methyltransferase inhibitory compound, to determine whether there is any difference m biological activity between the isomers. This approach is advantageous over the alternative, separating the racemic mixture into its stereoisomenc components Regardless of how a putative adenme DNA methyltransferase is prepared according to the invention, the compound is analyzed for both adenme and cytosine- specific DNA methyltransferase activity. Susceptible bacteria (known to express an adenme DNA methyltransferase) are grown in the presence and absence of the inhibitory compound, and the extent of growth inhibition in the presence of the compound is determined relative to growth m the absence of the compound. The mechanism of action (i e , inhibition of adenme DNA methyltransferase) is verified for each growth-inhibitory compound by filter-bmdmg radioassay using hemimethylated DNA, tπtiated S-adenosyl methionme (C 3 H 3 ) and a punfied adenme DNA methyltransferase according to International Application Publication No. WO98/12206. Compounds of the invention can exist as tautomers m solution When structures and names are given for one tautomeπc form the other tautomeπc form is also included in the invention. Representative compounds of the present invention include, but are not limited to the compounds disclosed herein and their pharmaceutically acceptable acid and base addition salts. In addition, if the compound of the invention is obtained as an acid addition salt, the free base can be obtained by basifymg a solution of the acid salt. Conversely, if the product is a free base, an addition salt, particularly a pharmaceutically acceptable addition salt, may be produced by dissolving the free base in a suitable organic solvent and treating the solution with an acid, m accordance with conventional procedures for prepaπng acid addition salts from base compounds. The present invention also encompasses the acylated prodrugs of the compounds of the invention. Those skilled in the are will recognize various synthetic methodologies which may be employed to prepare non-toxic pharmaceutically acceptable addition salts and acylated prodrugs of the inventive compounds. By "alkyl", "lower alkyl", and "C-.-C 6 alkyl" in the present invention is meant straight or branched chain alkyl groups having 1-6 carbon atoms, such as, methyl, ethyl, propyl, isopropyl, H-butyl, sec-butyl, tert-butyl, pentyl, 2-pentyl, isopentyl, neopentyl, hexyl, 2-hexyl, 3-hexyl, and 3-methylpentyl. By "alkoxy", "lower alkoxy", and "Cι-C 6 alkoxy" in the present invention is meant straight or branched chain alkoxy groups having 1-6 carbon atoms, such as, for example, methoxy, ethoxy, propoxy, isopropoxy, «-butoxy, sec-butoxy, tert-butoxy, pentoxy, 2-pentyl, isopentoxy, neopentoxy, hexoxy, 2-hexoxy, 3-hexoxy, and 3- methylpentoxy. By the term "halogen" in the present invention is meant fluorine, bromine, chlorine, and iodine. By "cycloalkyl", e. , C 3 -C 7 cycloalkyl, m the present invention is meant cycloalkyl groups having 3-7 atoms such as, for example cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, and cycloheptyl. In the C 3 -C 7 cycloalkyl groups, preferably m the C 5 -C7 cycloalkyl groups, one or two of the carbon atoms forming the nng can optionally be replaced with a hetero atom, such as sulfur, oxygen or nitrogen. Examples of such groups are pipeπdmyl, piperazmyl, morpholmyl, pyrrohdmyl, lmidazohdmyl, oxazohdmyl, azaperhydroepmyl, oxazaperhydroepmyl, oxepanyl, oxazaperhydromyl, and oxadiazaperhydromyl. C 3 and C 4 cycloalkyl groups having a member replaced by nitrogen or oxygen include azmdinyl, azetidmyl, oxetanyl, and oxiranyl By "aryl" is meant an aromatic carbocychc group having a single nng (e.g., phenyl), multiple rings (e.g., biphenyl), or multiple condensed nngs m which at least one is aromatic, (e.g., 1,2,3,4-tetrahydronaphthyl, naphthyl, anthryl, or phenanthryl), which is optionally mono-, di-, or tπsubstituted with, e.g., halogen, lower alkyl, lower alkoxy, lower alkylthio, trifluoromethyl, lower acyloxy, aryl, heteroaryl, and hydroxy. Prefened aryl groups include phenyl and naphthyl, each of which is optionally substituted as defined herein. By "heteroaryl" is meant one or more aromatic ring systems of 5-, 6-, or 7- membered rings containing at least one and up to four heteroatoms selected from nitrogen, oxygen, or sulfur. Such heteroaryl groups include, for example, thienyl, furanyl, thiazolyl, lmidazolyl, (ιs)oxazolyl, pyπdyl, pyπmidmyl, (ιso)qumolmyl, napthyridmyl, benzimidazolyl, benzoxazolyl. Preferred heteroaryls are thiazolyl, pyπmidmyl, preferrably pyπmιdm-2-yl, and pyπdyl. Other preferred heteroaryl groups include 1 -lmidazolyl, 2-thιenyl, 1-, or 2- qumolmyl, 1-, or 2- lsoqumolmyl, 1-, or 2- tetrahydro lsoqumolmyl, 2- or 3- furanyl and 2- tetrahydro furanyl. The bacterial growth inhibitory, adenme DNA methyltransferase inhibiting compounds of the invention are provided either from combinatonal hbranes, solid phase synthesis, "rational" drug design, or conventional synthesis as described herein. Construction of Combinatorial Libraries Combinatorial libraries are prepared according to methods understood by those with skill in the art. For the smgle substitution libraries (N6 and N9), the individual substituents are used m separate reaction mixtures to produce each of the punne deπvatives described herein. In the combination libraries (N6/N9 herein), on the other hand, one position (typically N9) is typically reacted with a particular substituent, and then a mixture of substituents (most preferably 3) used to deπvatize the other reaction position (typically C6). The reactions are performed on a scale adapted to economically producing sufficient product for testing. Preferably, reactions are performed m parallel, for example using a 96-well plate with each well having a sufficiently small volume (100- 500μL) to minimize the amount of reagents required. The use of this type of reaction vessel also facilitates parallel handling and analysis, including automated versions of such processes. a. N6 Libraries The following conditions were developed to synthesize analogues of adenme substituted at the N-6 position. In one reaction scheme, 6-chloropurme is reacted at 85°C overnight with a primary or secondary amme in tnethylamme in «-butanol. Alternatively, these reagents are reacted in potassium carbonate in dimethylformamide at 85°C overnight. This synthesis is descnbed in Reaction Scheme 1. Reaction Scheme 1 R" and R"' are lower alkyl, hetero atom-substituted lower alkyl, aryl, heteroaryl or substituted aryl or heteroaryl, as exemplified by the compounds set forth below Any primary or secondary am e can be used in this reaction Preferred embodiments of primary or secondary amines used in these reactions is as follows histamme dihydrochlonde norphenylephnne hydrochlonde 1 ,2-dιammopropane 5-ammo-l,3,3-tπmethylcyclohexanemethyl-amme 3 -lsopropoxypropylamme diphenylbonnic acid, ethanolamme ester 2-(2-ammoethylamιno)-ethanol tetrahydrofurfurylamme 5-methyltryptamme hydrochlonde 3 , 3 -diphenylpropylamme 1 -(3-ammopropyl)-2-pyπohdmone 2-(2-ammoethyl)- 1 -methylpyrrohdme 2-(ammomethyl)benzιmιdazole dihydro-chlondehyrate 2,2,2-tπfluoroethylamιne hydrochlonde L-carnosme (R)-(-)- 1 -ammo-2-propanol 2-(l-cyclohexenyl)ethylamme 4-(tπfluoromethyl)benzylamme 2, 5-dιchloroamylamme hydrochlonde (+/-)-4-ammo-3-hydroxybutyπc acid N,N-dιmethylethylenedιamme 3,3-dιmethylbutylamme 1 ,4-dιamιno-2-butanone dihydrochlonde ammomethylbenzoic acid aminohydroxymethylpropane diol 2-(ammoethyl)pyndme ammobutanol adamantamme ammohexanoic acid N-benyzylethanolamme ethyl-6-ammobutyrate hydrochlonde ethylenediamine 2-cyclohex- 1 -enylethylamme Some of these amines produce different regioisomers, i e , for some compounds the amine can be added to the C6 position of 6-chloropuπne in different orientations, depending on which reactive moiety comprising the amme covalently bonds to C6 However, the occurrence of these regioisomers is not deletenous, since it merely increases the number of candidate compounds m the library. b. N9 Libraries N9 hbranes were prepared using the following reaction schemes. It was found that Path A of Reaction Scheme 2 did not yield product with all organic halides (R 1V X or R X); alternative Path B was found to form product throughout the range of organic halides tested. In each alternative, the organic halide was reacted with punne (either adenme or 6-chloropuπne) at 45°C overnight in potassium carbonate in dimethylformamide. In Path B, however, the N9-deπvatιzed 6-chloropurme was converted to N9-deπvatιzed adenme by reaction of the product of the first reaction with ammonium hydroxide at 85°C overnight. Both reactions are performed sequentially in the same reaction mixture. Reaction Scheme 2 R 1V is lower alkyl, hetero atom-substituted lower alkyl, aryl, heteroaryl or substituted aryl or heteroaryl, as exemplified by the compounds set forth below. The products of Path B were analyzed by HPLC and found to be a mixture of N-9 and N- 7 substituted adenme analogues; there may also be N-1 and N-3 substituted analogues in certain reaction mixtures. As discussed above, the advantage of these side products is that their existence simply increases the number of candidate molecules m the library. Any organic halide can be used m this reaction Preferred embodiments of organic halides used in these reactions is as follows methyl 4-ιodobutyrate 1 -bromo-3 -phenylpropane cmnamyl bromide 2-chloroethylphosphonιc acid ethyl 2-(2-chloroacetamιdo)-4-thιazole-acetate 4-(2-chloroethyl)morpholme hydrochlonde (2 -bromoethyl)tπmethylammonιum bromide 4-chlorophenyl 2-bromoethyl ether N-(3 -bromopropyl)pthahmιde 2-chloroethyl isocyanate 2-chloro-N, N-dimethylacetoacetamide 3 -chloro-2-hydroxypropyl methacrylate 2-bromo-2 ' -hydroxy-5 ' -nitroacetanihde 3 -(2-bromoethyl)mdole 5-chloro-2-pentanone ethylene ketal 2-chloroethyl ethyl sulfide 3-chloro-N-hydroxy-2,2-dιmethyl-propιonamιde L-l-p-Tosylammo-2-Phenylethyl chloromethyl ketone 2-(2-bromoethyl)-l ,3-dιoxolane ethyl 2-(bromomethyl)acrylate 2-(2-(2-chlorethyoxy)ethoxy)ethanol (3 -chloropropyl)tnmethoxysιlane chloramphemcol 4-(chloromethyl)benzoιc acid bromoethylamme hydrochlonde epibromohydrm lodopentane benzyl bromide c. N6/N9 Combination Libraries Having developed the reaction schemes shown above for N6 and N9 hbranes, Reaction Scheme 3 combination libraries having substituents at both the C6 ammo and N9 positions were prepared using a modification of Path B of Reaction Scheme 2 6-Chloropunne was reacted with an organic halide as described above at 45°C overnight in a solution of potassium carbonate in dimethylformamide. Thereafter, a primary or secondary amme is added to the reaction mixture (although the scheme depicts a primary amme, primary or secondary amines are useful m Reaction Scheme 3) and compound (7) prepared by reaction at 85°C overnight. In this modification, the primary or secondary amme is substituted in the reaction for ammonium hydroxide show in Path B of Reaction Scheme 2. Any organic halide can be used m the first step of this reaction. Prefened embodiments of organic halides used in these reactions is as follows- methyl 4-ιodobutyrate 1 -bromo-3 -phenylpropane cmnamyl bromide 2-chloroethylphosphonιc acid ethyl 2-(2-chloroacetamιdo)-4-thιazole-acetate 4-(2-chloroethyl)morphohne hydrochlonde (2-bromoethyl)tπmethylammonιum bromide 4-chlorophenyl 2-bromoethyl ether N-(3 -bromopropyl)pthalιmιde 2-chloroethyl isocyanate 2-chloro-N, N-dimethylacetoacetamide 3-chloro-2-hydroxypropyl methacrylate 2-bromo-2 ' -hydroxy-5 ' -mtroacetanilide 3-(2-bromoethyl)mdole 5-chloro-2-pentanone ethylene ketal 2-chloroethyl ethyl sulfide 3-chloro-N-hydroxy-2,2-dιmethyl-propιonamιde L-l-p-Tosylammo-2-Phenylethyl chloromethyl ketone 2-(2-bromoethyl)-l,3-dιoxolane ethyl 2-(bromomethyl)acrylate 2-(2-(2-chlorethyoxy)ethoxy)ethanol (3 -chloropropy l)tnmethoxy silane chloramphenicol 4-(chloromethyl)benzoιc acid bromoethylamme hydrochlonde epibromohydnn lodopentane benzyl bromide Any primary or secondary amine can be used in the second step of this reaction Prefened embodiments of primary or secondary ammes used in these reactions is as follows: histamme dihydrochlonde norphenylephnne hydrochlonde 1 ,2-dιammopropane 5-ammo-l,3,3-tnmethylcyclohexanemethyl-amme 3 -isopropoxypropylamine diphenylboπnic acid, ethanolamme ester 2-(2-ammoethylammo)-ethanol tetrahydrofurfurylamme 5-methyltryptamme hydrochlonde 3 , 3 -diphenylpropy lamme 1 -(3 -ammopropyl)-2-pyrrohdmone 2-(2-ammoethyl)-l-methylpyπohdme 2-(ammomethyl)benzιmιdazole dihydro-chloπdehyrate 2,2,2 -tπfluoroethylamme hydrochlonde L-carnosine (R)-(-)- 1 -ammo-2-propanol 2-( 1 -cyclohexenyl)ethylamme 4-(tπfluoromethyl)benzylamme 2, 5-dιchloroamylamme hydrochlonde (+/-)-4-ammo-3 -hydroxybutyπc acid N,N-dιmethylethylenedιamme 3,3-dιmethylbutylamme 1 ,4-dιammo-2-butanone dihydrochlonde ammomethylbenzoic acid ammohydroxymethylpropane diol 2-(ammoethyl)pyπdme ammobutanol adamantamine ammohexanoic acid N-benyzylethanolamme ethyl-6-ammobutyrate hydrochlonde ethylenediamme 2-cyclohex- 1 -enylethylamme This chemistry can be repeated with any halide combination with any amme to give any adenme analogues of this type. As with Reaction Schemes 1 and 2, different regioisomers are produced with certain N6 ammes, and substituted punnes are produced at the Nl, N3 and N7 positions as well as at the N9 position by reaction with organic halides. To obtain a complete combinatorial library m a reasonable time the chemistry was performed m a 96 well plate using 80 wells at a time (Columns 1-10, Rows A-H). A single halide was added to the reaction mixture in each row and the first portion of the reaction shown in Reaction Scheme 3 performed overnight as describe above. Sets of three different ammes were then added to each well m each of columns 1-7; wells m columns 8, 9 and 10 are prepared containing only one amme, particularly for those species that would have a tendency to react with any additional ammes in the reaction mixture. The second portion of the reaction performed overnight As a result, most of the wells contained a mixture of compounds, each well comprising a set of punne derivatives with a particular substituent at the N9 position and one of three different substituted ammo groups at C6. It is recognized that certam of the reaction mixtures will be deficient in one, two or all three of the C6 substituents, depending on the reactivity of each ammo group with N9-deπvatιzed 6-chloropurme. In addition, the reaction mixtures contain different regioisomers of these products. Finally, reaction between combinations of the ammes reacting directly with the halides without reacting with 6-chloropurme is possible. These product compounds in each reaction mixture were tested as mixtures for antibacterial, and specifically adenme DNA methyltransferase inhibitory activity. Mixtures showing positive results were separated to identify the compound responsible for the result. Screening of Combinatorial Libraries Reaction products from each of the libraries prepared as described above were screened using both in vivo and in vitro screening methods. In vivo screening methods involved assays for growth inhibition of bacterial cells expressing an adenme DNA methyltransferase essential for cell growth. Advantageously, these screening methods utilize more than one species of bactena, to identify lead candidates having the broadest spectrum of antibiotic activity In certam embodiments, the putative inhibitors are first screened against samples of gram positive and gram-negative bactena; Caulobacter cresentus and Bacillus subtihs are advantageous examples. Additionally advantageous bacterial species for detecting in vivo adenme DNA methyltransferase activity include Hehcobacter pylori, Agrobacter tumefaciens, Brucella abortus and Bacillus anthracis In these assays, bacterial cultures such as Caulobacter were grown in an appropnate bacterial culture media such as peptone yeast extract (PYE) media (DIFCO) overnight to saturation. Aliquots of this culture were diluted to a concentration having an optical density at 600nm (OD 6 oo) of about 0.05 The assay is conveniently performed in 96 well microtitre plates, particularly using libraries prepared in such plates. Using these microtitre plates, an equal amount (100-500μL) of the diluted bacterial culture was placed in 88 of the 96 wells of the microtitre plate; the remaining 8 wells were used as negative (no bactena) controls. Eight of the wells were used as positive (no added test compound) controls. For library screening, bacterial aliquots of 146μL can be used per well with the addition of 4μL of combinatorial library sample A different mixture of library compounds was added to each of the remaining 80 wells per plate, and the cells grown for 24h at 37°C. Bacterial cell growth was monitored at intervals using a microplate reader to monitor cell growth; cell growth can be monitored by measuring the OD 630 . Wells containing cells growing more slowly than control wells were used to identify corresponding combinatorial library reaction mixtures, which were then synthesized and tested individually to determine the identity of the inhibitory compound. Using these methods, candidate compounds that inhibited bacterial cell growth at an estimated concentration of <100μM were identified. Candidate compounds identified from these hbranes include 6-N-(dιphenylbonnιc ester)-ethyl-adenme, 6-N- (diphenylbonmc ester)-ethyl-9-(2-(4-morpholmyl)-ethyl)-adenιne, 6-N-(dιphenylbonnιc ester)-ethyl-9-(3-(N-phthaloyl)-amιnopropyl)-adenme, 6-N-(dιphenylbormιc ester)-ethyl- 9-(2-(2-(2-hydroxyethoxy)ethoxy)ethyl)-adenme, and 6-N-(dιphenylbormιc ester)-ethyl- 9-(ethyl-2-acrylate)-methyl-adenme. fn vitro assays were performed directly on reaction mixtures from the combinatorial libraries of the invention, or on candidate compounds identified from the in vivo screening assays described above. These assays are of two types, using purified CcrM methyltransferases from Caulobacter cresentus or Brucella abortus, or using commercially-available preparations of bacterial dam methylases and dcm methylases. In these assays, a synthetic hemimethylated 45/50 DNA substrate (as disclosed m Berdis et al, 1998, Proc Natl Acad Sci USA 95: 2874-2879) was incubated with the combinatonal library sample containing a putative inhibitor and the methyltransferase at 30°C, and the methyltransferase reaction initiated by the addition of 3 H-labeled S- adenosylmethionme (wherein the radioisotope label comprises the transferred methyl group). Inhibition is detected by comparing the amount of radiolabel incorporated m controls where the reaction was performed m the presence and absence of combinatorial library samples; inhibitors cause a reduction the amount of methylated, 3 H-labeled DNA collected on a DE81 filter and radioactivity quantified by liquid scintillation. Using this assay, four additional compounds from the N6 library were detected that completely inhibited in vitro DNA methylation at an estimated concentration of about 500μM. These compounds (having the structures shown below) were ribose forms of adenosme having either diphenylbonnic acid ethanolamme ester or 3,3- diphenyhsopropyl groups covalently linked to the C6 ammo group of the adenme ring: One reaction mixture from the synthesis of the structures shown above from the N-6 adenme library was further analyzed and found to inhibit cell growth at < 50μM. An approximate K, of 1 μM was measured (assuming the theoretical maximum concentration in the well) for this compound, having the structure: Selected reaction mixtures from the N-9 adenme library were tested for inhibition of adenme DNA methyltransferase activity using this assay Compounds obtained in the synthesis having N9 substituted with 3-ethylmdole or 2-ethyl-l,3- dioxolane (having the structures shown below) were found to be inhibitors of adenme DNA methyltransferase activity at 500 μM In vitro assays using dcm methyltransferases were performed essentially as described using commercially-available methyltransferase from Hemophilus hemolyticus (New England Biolabs, Beverly, MA) and pUC 18 DNA as substrate, this assay can also be performed with other commercially-available dcm methyltransferases, for example, from Arthtobacter luteus, Bacillus amyloliquifaciens H, Hemophilus aegyptius, Hemophilus parainfluenza, or Moraxella species In these assays, adenme specificity is demonstrated by detecting little or no inhibition of the dcm methyltransferase, so that the amount of methylated, 3 H-labeled DNA collected on a DE81 filter and radioactivity quantified by liquid scintillation is the same m the presence or absence of the combinatonal library mixtures or putative adenme-specific inhibitor Alternatively, assays using dam methylases, for example from Escherichia coh, are performed wherein inhibition is demonstrated by a reduction in the amount of methylated, 3 H-labeled DNA was collected on a DE81 filter and radioactivity quantified by liquid scintillation. Synthesis of "Rational Design" Adenine DNA Methylase Inhibitors a. Active site analogues "Rational design" of adenine DNA methyltransferases depends on the assumption that the active site of the enzyme contains specific binding sites for the adenine moiety to be modified as well as the S-adenosyl methionme methyl donor, as shown m Figure 2. Compounds that fit within the active site of the methyltransferase are prefened , and molecules that mimic the putative "transition state" where the methyl transfer activity of the enzyme occurs are particularly prefened. Four such transition state analogues were prepared and assayed in vitro for adenme DNA methyltransferase activity. The rational design compounds have the structure: 1 Ri = OH R 2 = H 2 R, = H R 2 = H 3 Ri= OH R 2 = P0 3 4 R, = H R 2 = P0 3 and were synthesized using Reaction Scheme 6, shown below. Reaction Scheme 6 These compounds were assayed as described above for adenine DNA methyltransferase activity. These compounds were found to inhibit CcrM with the K,'s indicated in Table I. The K,'s for compounds 2 and 4 were calculated from a Dixon plot of the data measured at concentration of inhibitors from 0 - 150 μM for 3 and 0 - 80 μM for compound 4. K,'s for 1 and 2 are estimated from IC 50 's. Table I: Kj's of Compounds 1-4 Solid Phase Synthesis of Adenine DNA Methyltransferase Inhibitors Adenine DNA methyltransferase inhibitors of the invention are also advantageously synthesized using solid phase synthetic methods well known in the art See Bun , ibid In prefened embodiments, solid phase synthesis complements combinatonal library synthesis as described herein by allowing access to a larger number of library compounds for screening. This synthetic method has the additional advantages of being easier to handle and easier to purify, since they are attached to the resm by chemically- labile groups that can be specifically cleaved For example, compound (8) was identified from the N6 library having relatively low (mM range) inhibitory activity: (8) (9) Using these results, second generation hbranes were developed using solid phase chemistry to modify inhibitor (8) and its related analogue (9). For example, compound (8) can be denvatized from the terminal amme and from the N-9 position to produce inhibitors that are designed to interact with the S-adenosyl methionme (SAM) and DNA binding sites respectively. Such derivatives can be prepared to target either portion of the methyltransferase active site modifications of the N9 position are specific to the adenme binding site, while modifications of the C6 amme is specific for the SAM site. Solid phase chemistry was performed using reaction schemes illustrated by Reaction Scheme 4: Reaction Scheme 4 These experiments used a commercially-available methoxyphenyl formyl resm to perform a reductive animation with a protected diamme (11), where R v is hydrogen or C0 2 P" (where P' and P" are protecting groups). This reaction yields the secondary amme (12). Addition of 6-chloropurme results in resm-bound adenine adduct (13), permitting the remaining functional groups to be denvatized on the resm. The adducts can be removed from the resm according to art-recognized methods, such as treatment with tπfluoroacetic acid, either m concentrated form or as a 5% solution m dichloromethane. Reaction scheme 4 illustrates an embodiment where the ammo substituent contains a chiral center (i.e , it exists as a pair of stereoisomers). However, only one of these stereoisomers may have biological activity. In order to avoid having to separate diastereomenc forms of the compounds of the invention, the following Reaction Scheme 5 can be used: Reaction Scheme 5 Me Si 66% (20) This synthesis can be used to detect adenine DNA methyltransferase inhibitory activity in compounds compnsmg a racemic mixture of a chiral center (as occurs m 5 aspartic acid (16)); this synthesis can be repeated with commercially pure D- or L- aspartic acid to obtain optically-pure embodiments in the event that one stereoisomer has significantly more activity that the other. As shown in Reaction Scheme 5, D,L- Aspartic acid (16) was treated with benzyl chloro formate to yield the N-carboxybenzyl protected aspartic acid (17). The α-carboxyhc acid was then protected as an oxazohdmone (18) 10 using paraformaldehyde. A Curtius reanangement was performed on the remaining β- carboxyhc acid using diphenylphosphoryl azide to give the isocyanate (19). These reactions compnse art-recognized synthetic methods, from this point forward the chemistry is novel. The isocyanate (19) was trapped using tπmethylsilyl ethanol (20) to give the Teoc (tnmethylsilylethoxycarbonyl) protected amme (21).The oxazohdmone 15 was ring opened using sodium methoxide to give the methyl ester (22). The Teoc protecting group was then removed using tπfluoroacetic acid to yield the mono protected diamme (23). Compounds (23) and (11, where R v = H, P' = CBz) have been used to synthesize the resm bound adenine analogues (13), having R v = C0 2 Me, P' = CBz, and (13), R v = H, 20 P' = CBz, respectively. Uses of the Compounds of the Invention The invention also provides embodiments of the compounds disclosed herein as pharmaceutical compositions. The pharmaceutical compositions of the present invention can be manufactured m a manner that is itself known, e g , by means of a conventional mixing, dissolving, granulating, dragee-makmg, levigating, emulsifying, encapsulating, entrapping or lyophihzmg processes. Pharmaceutical compositions for use in accordance with the present invention thus can be formulated in conventional manner using one or more physiologically acceptable carriers comprising excipients and auxiliaries that facilitate processing of the active compounds into preparations that can be used pharmaceutically Proper formulation is dependent upon the route of administration chosen. Non-toxic pharmaceutical salts include salts of acids such as hydrochlonc, phosphoric, hydrobromic, sulfuπc, sulfmic, formic, toluenesulfomc, methanesulfonic, nitic, benzoic, citnc, tartaπc, maleic, hydroiodic, alkanoic such as acetic, HOOC-(CH 2 ) n - CH 3 where n is 0-4, and the like. Non-toxic pharmaceutical base addition salts include salts of bases such as sodium, potassium, calcium, ammonium, and the like. Those skilled in the art will recognize a wide variety of non-toxic pharmaceutically acceptable addition salts. For injection, the compounds of the invention can be formulated m appropriate aqueous solutions, such as physiologically compatible buffers such as Hanks's solution, Rmger's solution, or physiological salme buffer. For transmucosal and franscutaneous administration, penefrants appropriate to the barner to be permeated are used in the formulation. Such penefrants are generally known in the art. For oral administration, the compounds can be formulated readily by combining the active compounds with pharmaceutically acceptable earners well known in the art. Such earners enable the compounds of the invention to be formulated as tablets, pills, dragees, capsules, liquids, gels, syrups, slurnes, suspensions and the like, for oral ingestion by a patient to be treated. Pharmaceutical preparations for oral use can be obtained with solid excipient, optionally grinding a resulting mixture, and processing the mixture of granules, after adding suitable auxihaπes, if desired, to obtain tablets or dragee cores. Suitable excipients are, m particular, fillers such as sugars, including lactose, sucrose, mannitol, or sorbitol; cellulose preparations such as, for example, maize starch, wheat starch, rice starch, potato starch, gelatin, gum tragacanth, methyl cellulose, hydroxypropylmethylcellulose, sodium carboxymethylcellulose, and/or polyvmylpyrrohdone (PVP). If desired, disintegrating agents can be added, such as the cross-linked polyvinyl pynohdone, agar, or algmic acid or a salt thereof such as sodium algmate Dragee cores are provided with suitable coatings. For this purpose, concentrated sugar solutions can be used, which can optionally contain gum arable, talc, polyvinyl pynohdone, carbopol gel, polyethylene glycol, and/or titanium dioxide, lacquer solutions, and suitable organic solvents or solvent mixtures. Dyestuffs or pigments can be added to the tablets or dragee coatings for identification or to characterize different combinations of active compound doses. Pharmaceutical preparations that can be used orally include push-fit capsules made of gelatin, as well as soft, sealed capsules made of gelatin and a plasticizer, such as glycerol or sorbitol. The push-fit capsules can contain the active ingredients in admixture with filler such as lactose, binders such as starches, and/or lubricants such as talc or magnesium stearate and, optionally, stabilizers In soft capsules, the active compounds can be dissolved or suspended in suitable liquids, such as fatty oils, liquid paraffin, or liquid polyethylene glycols. In addition, stabilizers can be added. All formulations for oral administration should be m dosages suitable for such administration. For buccal administration, the compositions can take the form of tablets or lozenges formulated in conventional manner. For administration by inhalation, the compounds for use according to the present invention are conveniently delivered in the form of an aerosol spray presentation from pressurized packs or a nebuhser, with the use of a suitable propellant, e.g., dichlorodifluoromethane, tnchlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas. In the case of a pressurized aerosol the dosage unit can be determined by providing a valve to deliver a metered amount. Capsules and cartridges of e g , gelatin for use m an inhaler or insufflator can be formulated containing a powder mix of the compound and a suitable powder base such as lactose or starch. The compounds can be formulated for parenteral administration by injection, e.g , by bolus injection or continuous infusion. Formulations for injection can be presented in unit dosage form, e.g , m ampoules or m multi-dose containers, with an added preservative. The compositions can take such forms as suspensions, solutions or emulsions m oily or aqueous vehicles, and can contain formulatory agents such as suspending, stabilizing and/or dispersing agents. Pharmaceutical formulations for parenteral administration include aqueous solutions of the active compounds in water-soluble form. Additionally, suspensions of the active compounds can be prepared as appropnate oily injection suspensions. Suitable hpophilic solvents or vehicles include fatty oils such as sesame oil, or synthetic fatty acid esters, such as ethyl oleate or tπglyceπdes, or hposomes. Aqueous injection suspensions can contain substances that increase the viscosity of the suspension, such as sodium carboxymethyl cellulose, sorbitol, or dextran. Optionally, the suspension can also contain suitable stabilizers or agents that increase the solubility of the compounds to allow for the preparation of highly concentrated solutions. Alternatively, the active ingredient can be m powder form for constitution with a suitable vehicle, e.g , stenle pyrogen-free water, before use. The compounds can also be formulated in rectal compositions such as suppositories or retention enemas, e g , containing conventional suppository bases such as cocoa butter or other glyceπdes In addition to the formulations described previously, the compounds can also be formulated as a depot preparation. Such long acting formulations can be administered by implantation (for example subcutaneously or intramuscularly) or by intramuscular injection. Thus, for example, the compounds can be formulated with suitable polymeric or hydrophobic materials (for example as an emulsion in an acceptable oil) or ion exchange resms, or as spanngly soluble derivatives, for example, as a sparingly soluble salt. A pharmaceutical carrier for the hydrophobic compounds of the invention is a cosolvent system compnsmg benzyl alcohol, a nonpolar surfactant, a water-miscible organic polymer, and an aqueous phase. The cosolvent system can be the VPD co- solvent system. VPD is a solution of 3% w/v benzyl alcohol, 8% w/v of the nonpolar surfactant polysorbate 80, and 65% w/v polyethylene glycol 300, made up to volume in absolute ethanol. The VPD co-solvent system (VPD:5W) consists of VPD diluted 1 :1 with a 5% dextrose m water solution. This co-solvent system dissolves hydrophobic compounds well, and itself produces low toxicity upon systemic administration. Naturally, the proportions of a co-solvent system can be vaned considerably without destroying its solubility and toxicity characteristics. Furthermore, the identity of the co- solvent components can be varied: for example, other low-toxicity nonpolar surfactants can be used instead of polysorbate 80; the fraction size of polyethylene glycol can be vaned; other biocompatible polymers can replace polyethylene glycol, e g polyvinyl pyrrolidone; and other sugars or polysacchaπdes can substitute for dextrose. Alternatively, other delivery systems for hydrophobic pharmaceutical compounds can be employed. Liposomes and emulsions are well known examples of delivery vehicles or earners for hydrophobic drugs. Certain organic solvents such as dimethylsulfoxide also can be employed, although usually at the cost of greater toxicity. Additionally, the compounds can be delivered using a sustamed-release system, such as semipermeable matrices of solid hydrophobic polymers containing the therapeutic agent. Various sustamed-release materials have been established and are well known by those skilled m the art. Sustamed-release capsules can, depending on their chemical nature, release the compounds for a few weeks up to over 100 days Depending on the chemical nature and the biological stability of the therapeutic reagent, additional strategies for protein and nucleic acid stabilization can be employed. The pharmaceutical compositions also can comprise suitable solid or gel phase earners or excipients. Examples of such earners or excipients include but are not limited to calcium carbonate, calcium phosphate, various sugars, starches, cellulose derivatives, gelatin, and polymers such as polyethylene glycols The compounds of the invention can be provided as salts with pharmaceutically compatible counteπons. Pharmaceutically compatible salts can be formed with many acids, including but not limited to hydrochloric, sulfuπc, acetic, lactic, tartaπc, malic, succinic, phosphonc, hydrobromic, sulfinic, formic, toluenesulfomc, methanesulfomc, nitic, benzoic, citric, tartaπc, maleic, hydroiodic, alkanoic such as acetic, HOOC-(CH 2 ) n - CH3 where n is 0-4, and the like. Salts tend to be more soluble in aqueous or other protomc solvents that are the conespondmg free base forms Non-toxic pharmaceutical base addition salts include salts of bases such as sodium, potassium, calcium, ammonium, and the like. Those skilled m the art will recognize a wide variety of non- toxic pharmaceutically acceptable addition salts. Pharmaceutical compositions of the compounds of the present invention can be formulated and administered through a variety of means, including systemic, localized, or topical administration. Techniques for formulation and administration can be found in "Remington's Pharmaceutical Sciences," Mack Publishing Co., Easton, PA. The mode of administration can be selected to maximize delivery to a desired target site in the body. Suitable routes of administration can, for example, include oral, rectal, transmucosal, franscutaneous, or intestinal administration; parenteral delivery, including intramuscular, subcutaneous, mtramedullary injections, as well as mfrathecal, direct mfraventπcular, intravenous, mtrapeπtoneal, mtranasal, or intraocular injections. Alternatively, one can administer the compound in a local rather than systemic manner, for example, via injection of the compound directly into a specific tissue, often in a depot or sustained release formulation Pharmaceutical compositions suitable for use in the present invention include compositions wherein the active ingredients are contained m an effective amount to achieve its intended purpose. More specifically, a therapeutically effective amount means an amount effective to prevent development of or to alleviate the existing symptoms of the subject being treated. Determination of the effective amounts is well withm the capability of those skilled in the art, especially m light of the detailed disclosure provided herein For any compound used in the method of the invention, the therapeutically effective dose can be estimated initially from cell culture assays, as disclosed herein. For example, a dose can be formulated animal models to achieve a circulating concenfration range that includes the EC50 (effective dose for 50% increase) as determined in cell culture, i e , the concenfration of the test compound which achieves a half-maximal inhibition of bacterial cell growth. Such information can be used to more accurately determine useful doses m humans. It will be understood, however, that the specific dose level for any particular patient will depend upon a variety of factors including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, route of administration, and rate of excretion, drug combination, the seventy of the particular disease undergoing therapy and the judgment of the prescnbing physician. For administration to non-human animals, the drug or a pharmaceutical composition containing the drug may also be added to the animal feed or dnnkmg water. It will be convenient to formulate animal feed and dnnkmg water products with a predetermined dose of the drug so that the animal takes in an appropriate quantity of the drug along with its diet. It will also be convenient to add a premix containing the drug to the feed or drinking water approximately immediately pπor to consumption by the animal. Prefened compounds of the invention will have certain pharmacological properties. Such properties include, but are not limited to oral bioavailabihty, low toxicity, low serum protein binding and desirable in vitro and in vivo half-lives. Assays may be used to predict these desirable pharmacological properties. Assays used to predict bioavailabihty include transport across human intestinal cell monolayers, including Caco-2 cell monolayers. Serum protein binding may be predicted from albumin binding assays. Such assays are described in a review by Oravcova et al. (1996, J. Chromat. B 611: 1-27). Compound half-life is inversely proportional to the frequency of dosage of a compound, fn vitro half-lives of compounds may be predicted from assays of microsomal half-life as described by Kuhnz and Gieschen (Drug Metabolism and Disposition, (1998) volume 26, pages 1120-1127). Toxicity and therapeutic efficacy of such compounds can be determined by standard pharmaceutical procedures cell cultures or experimental animals, e.g , for determining the LD50 (the dose lethal to 50% of the population) and the ED50 (the dose therapeutically effective in 50% of the population). The dose ratio between toxic and therapeutic effects is the therapeutic index and it can be expressed as the ratio between LD50 and ED50. Compounds that exhibit high therapeutic indices are prefened. The data obtained from these cell culture assays and animal studies can be used in formulating a range of dosage for use in humans. The dosage of such compounds lies preferably withm a range of circulating concentrations that include the ED50 with little or no toxicity. The dosage can vary withm this range depending upon the dosage form employed and the route of administration utilized. The exact formulation, route of administration and dosage can be chosen by the individual physician m view of the patient's condition. (See, e.g Fmgl et al, 1975, m "The Pharmacological Basis of Therapeutics", Ch.l, p.l). Dosage amount and interval can be adjusted individually to provide plasma levels of the active moiety that are sufficient to maintain bactenal cell growth inhibitory effects. Usual patient dosages for systemic administration range from 100 - 2000 mg/day. Stated in terms of patient body surface areas, usual dosages range from 50 - 910 mg/m /day. Usual average plasma levels should be maintained withm 0.1-1000 μM. In cases of local administration or selective uptake, the effective local concentration of the compound cannot be related to plasma concentration. The compounds of the invention are modulators of cellular processes in bactena that mfect plants, animals and humans. The pharmaceutical compositions of the adenine DNA methylfransferase inhibitory compounds of the invention are useful as antibiotics for the treatment of diseases of both animals and humans, including but not limited to actmomycosis, anthrax, bactenal dysentery, botulism, brucellosis, celluhtis, cholera, conjunctivitis, cystitis, diphtheria, bactenal endocarditis, epiglottitis, gastroententis, glanders, gononhea, Legionnaire's disease, leptospirosis, bacterial meningitis, plague, bacterial pneumonia, puerperal sepsis, rheumatic fever, Rocky Mountain spotted fever, scarlet fever, streptococcal pharyngitis, syphilis, tetanus, tularemia, typhoid fever, typhus, and pertussis. The disclosures m this application of all articles and references, including patents, are incorporated herein by reference. The following Examples are provided for the purposes of illustration and are not intended to limit the scope of the present invention. The present invention is not to be limited in scope by the exemplified embodiments, which are intended as illustrations of individual aspects of the invention. Indeed, various modifications of the invention in addition to those shown and described herein will become apparent to those skilled in the art from the foregoing description and accompanying drawings. Such modifications are intended to fall withm the scope of the appended claims. EXAMPLE 1 Preparation of Solution Phase Chemistry Libraries Solution phase combinatorial libraries as described above were prepared in a 96- well microtitre plate as follows. To each well in columns 1-7 was added K 2 C0 3 (7-10 mg) followed by DMF (140 μL) and the 6-chloropurme (30 μL of 0.5M solution in DMF). To each row was added a halide (15 μL of a 1M solution in DMF) selected from the list of halides disclosed herein. To each well m columns 8-10 was added K 2 C0 3 (3-5 mg) followed by DMF (180 μL) and the 6-chloropurme (10 μL of 0.5M solution m DMF). To each row was added a halide (5 μL of a 1M solution in DMF) selected from the list of halides disclosed herein. The microtitre plate was heated to 45 °C and reacted overnight. Reactions were cooled to room temperature and the second synthetic reaction performed as follows. To each well in columns 1-7 was added 3 different ammes(5 μL each of a 1M solution in DMF ) selected from the list of ammes dislosed herein. To each well m columns 8-10 was added one amme (5 μL each of a 1M solution in DMF) selected from the list of ammes dislosed herein. The microtitre plate was heated to 85°C and reacted overnight. Reactions were cooled to room temperature, each well was collected separately and the final volume of the solution was adjusted to 300 μL (replacing solvent lost to evaporation). The compounds produced in these reactions were then tested using the in vivo bacterial growth assays disclosed herein. EXAMPLE 2 Preparation of Adenine DNA Methyltransferase Inhibitors The combinatorial libraries of the invention were screened for adenine DNA methylfransferase inhibitory activity as described above. A compound displaying methyltransferase inhibiting activity and having the C6 ammo group covalently linked to diphenylbonmc acid ethanolamme ester was used as the base compound for preparing related analogues according to the following reaction scheme: Specifically, 6-chloropurme (1) was dissolved in dry DMF (about 0.3mmol/mL) under argon at room temperature. Potassium carbonate (K 2 C0 3 ; 2-3 equivalents) was added, followed by one equivalent of the alkyl halide (R-X). The reaction was heated to either 45°C or 95°C (if the halide does not react at the lower temperature) and stiπed for 18 hours. After this time, thm layer chromatography was performed (using 2%- 5% methanol in dichloromethane as solvent) and showed little or no starting material remaining in the reaction mixture. The reaction was cooled to room temperature and the solid removed by filtration and washed with dry dimethylformamide (DMF). The filtrate was obtained and any remaining DMF was removed in vacuo to give the crude product as an oil The product was purified by column chromatography on silica gel (using 2%- 5% methanol in dichloromethane as solvent). The N-9 regioisomer was eluted as a pure fraction before a combination of the N-9 and N-7 regioisomers that eluted as a mixture. The fractions containing the pure N-9 isomer were combined and the solvent was removed in vacuo to give the intermediate product 2b - 2e as a solid or an oil that solidified on standing. The final compounds were prepared as follows. 6-chloropurme (1) or 9-alkyl-6- chloropunne (2b - 2e) was dissolved m dry DMF (0.03-0.5 mmol/mL) under argon at room temperature. Potassium carbonate (K 2 C0 3 ; 1.5 - 2 equivalents) was added followed by one equivalent of diphenylbon c acid ethanolamme ester. The reaction was heated to 90-95°C and stored for 18 hours. The mixture was allowed to cool to room temperature and the solid was removed by filtration as described above. The structure of these compounds was confirmed using Η-NMR, I 3 C-NMR, and two-dimensional NMR spectroscopic methods such as HMQC and HMBC. Alternatively, 6-chloropuπne (1) or N-9 alkyl-6-chloropurme (2b - 2e) was dissolved in 1-butanol (O.lmmol/mL) under argon at room temperature. Three equivalents of diisopropylethylamme were added, followed by the addition of 1.2 equivalents of alkyl halide. This reaction mixture was heated to 1 10°C and stirred for 18 hours. The reaction was then cooled to room temperature and the solvent was removed in vacuo. The residue was punfied by column chromatography on silica gel (using a solution of 2% to 5% methanol in dichloromethane in solvent). The product fractions were collected and the solvent was removed in vacuo to leave a white solid. It is known in the art that the structure of the stanng material, diphenylbonmc acid ethanolamme ester, is cyclic and the boron is tetrahedral. Thus, cyclic and linear analogues of the adenine DNA methylfransferase inhibiting compounds of the invention may be advantageous for developing additional inhibitory compounds. For in vivo assays, the unpuπfied preparation, which contained residual DMF or dimethylsulfoxide (DMSO), was diluted to 16.7mM and used directly as a crude mixture. In vivo assays of bacterial cell growth inhibition were performed essentially as described above using a vanety of bacterial species. The compounds (3a) through (3e) showed the following results in these assays: Caulobacter cresentus Compound 3a - IC50 <25μM Compound 3b - IC 50 <25μM Compound 3c - IC50 <25μM Compound 3d - IC 50 <25μM Compound 3e - IC50 <25μM Brucella abortus Compound 3a - Almost complete cell death after 12 hours - lOOμM Compound 3b - Complete cell death after 12 hours - lOOμM Compound 3c - Cell growth inhibited from start - 1 OOμM Compound 3d - Cell growth inhibited from start - lOOμM Compound 3e - Cell growth inhibited from start - lOOμM Helicobacter pylori Compound 3a - IC50 <25μM Compound 3b - IC50 <25μM Compound 3c - IC5 0 between 25-1 OOμM Compound 3d - IC 50 between 25-1 OOμM Compound 3e - IC 50 = 25 μM Agrobacterium tumefaciens Compound 3a - IC 50 >100μM Compound 3b - IC 50 = 25 μM Compound 3c - IC50 = 25 μM Compound 3d - IC5 0 «25μM Compound 3e - IC 50 « 25 μM Bacillus subtilis Compound 3a - IC 50 between 10-50μM Compound 3b - IC 50 between 1-1 OμM Compound 3c - IC 50 between l-10μM Compound 3d - IC 50 between 10-50μM Compound 3e - not tested In addition, the following results were obtained using in vitro adenine DNA methylfransferase inhibition assays. CcrM Compound 3a - Complete inhibition at lOOμM Compound 3b - Complete inhibition at lOOμM Compound 3c - Complete inhibition at lOOμM Compound 3d - Complete inhibition at lOOμM Compound 3e - Complete inhibition at lOOμM dam methylase (E. coli) Compound 3a - Complete inhibition at lOOμM Compound 3b - Complete inhibition at 1 OOμM Compound 3c - Complete inhibition at lOOμM Compound 3d - Complete inhibition at lOOμM Compound 3e - Complete inhibition at lOOμM dcm methyltransferase (Hhal) Compound 3a - No inhibition at all at 5 OOμM Compound 3b - No inhibition at all at 500μM Compound 3c - No inhibition at all at 500μM Compound 3d - No inhibition at all at 500μM Compound 3e - No inhibition at all at 500μM These results demonstrate that compounds (3a) through (3e) are adenme-specific DNA methyltransferases with no detectable dcm crossreactivity. EXAMPLE 3 Preparation of Adenine DNA Methyltransferase Inhibitors Additional adenine DNA methylfransferase inhibitors were developed by optimization of leads found during screening of the combinatorial libraries described above 1 6-(2-diphenylmethylcyclopentylammo)punne (Compound 73) 6-chloropurme was combined with S-(-)-2-(dιphenylmethyl)-pyrrohdme m n- butanol with two equivalents of diisopropylethylamme (N(ιPr) 2 Et) The reaction was heated to 105QC and allowed to react for 24 h Solvent was removed from the reaction mixture in vacuo and the crude product purified by silica gel chromatography 2 6-(2-dιphenylhvdroxymethylcyclopentylammo)punne (Compound 71) 6-chloropuπne was combined with R-(+)-α,α-dιphenyl-2-pynohdιnemethanol in n-butanol with two equivalents of N(ιPr) 2 Et The reaction was heated to 95 DC and allowed to react for 24 h Solvent was removed from the reaction mixture in vacuo and the crude product punfied by silica gel chromatography N(iPr) 2 Et, n-butanol 3. 2-dιphenylpynole (Compound 76) 2-pynohdmone was combined with dimethyl sulphate m benzene and refluxed for 3h. After punfication including distillation, the resulting 2-methylιmmo ester pynolidme was combined with excess lithium phenoxide (PhLi) m dry ether at room temperature for 18h to yield the title compound PhLi/ Ether 4. 6-ammo-4(2 -diphenylbonmc ester) ethylammo pynmidme (Compound III168) 4-ammo-6-hydroxy-2-thιopynmιdme was treated with Raney nickel (RaNi) in water and ammonia and heated to reflux for 2h. Punfication afforded the 4-ammo-6- hydroxypynmidme, which was combined with phosphorus oxychlonde and N,N- diethylanilme and heated at reflux for 4h to give 4-ammo-6-chloropynmιdme. This product was combined with diphenylbonmc acid ethanolamme ester in toluene with diisopropylethylamme and heated to reflux overnight to produce the title compound. toluene/ (ιPr)2EtN III168 5. 4-ammo-5(2-dιphenylbormιc ester ethylιmmoester)ιmιdazole (Compound III170) 4-ammo-5-ιmιdazolecarboxamιde hydrochlonde was heated to reflux in phosphorus oxychlonde for 3.5hs and after purification gave 4-amιno-5-mtnle imidazole. This was resuspended in ethanol saturated with HCl overnight and purification gave 4-ammo-5-ethyhmmo ester imidazole hydrochlonde. This was combined with diphenylbonmc acid ethanolamme ester m tetrahydrofuran (THF) overnight to yield the title compound. POC-- 3 , reflux EtOH, HCl III170 EXAMPLE 4 Second Generation Libraries Two "second generation libraries were prepared based on the compounds: (8) (9) The first second generation library ("library A") was constructed as shown below from parent compound 78. Compound 78 was combined with one equivalent of aldehyde (or ketone) in methanol at 25 DC in a heater-shaker. Reactions were set up in duplicate. After reaction for one hour, BH 3 -resin was added and the mixture was allowed to react overnight. To one set of the reaction was added a second equivalent of one of the following aldehydes: cyclohexanecarboxaldehyde; 3-furaldehyde; 1 -mefhyl-2-pyπolecarboxaldehyde; hydrocmnamaldehyde; 4-pyπdme carboxaldehyde; 2-phenylpropπonaldehyde; phenylacetaldehyde; m-anisaldehyde; heptaldehyde; 3 -nitrobenzaldehyde; 3 -phenylbutyraldehyde; 3-pyπdylacetaldehyde N-oxide; ethyl leveulmate; ethyl-2-ethylacetoacetone; ethyl-4-acetylbutyrate; ethyl propnonylacetate; ethyl 2-benzylacetoacetone; 1 -phenyl-2-pentanone; 1 -carbethoxy-4-pιpendone; N-acetonylphthahmide; 2-fluorophenylacetone; 4-(3-oxobutyl)phenylacetate. The entire plate was then allowed to react for a further 24 hrs. The compounds generated are either monoalkylated, with R=H, R - alkyl, aryl or dialkylated with R=R'=alkyl or aryl. The other second generation library ("library B") was constructed by reacting the parent compound III142 with the ammes used to construct the N6 library and set forth above m the presence of tπmethyl aluminum (AlMe 3 ) m dichloromethane at 50 DC overnight. The solvent was removed by evaporation, and the residue was dissolved m acetonitnle and treated with tnmethylsilyl iodide overnight. Reactions were worked up by adding methanol, evaporating the solvents, partitioning the residue between ether and water/acetic acid (7:3) and extracting the product into the aqueous layer. Second Generation Library A Second Generation Library B EXAMPLE 5 Compounds based on Diphenyl Borinic Esters Based on the results disclosed above, one common component of several of the adenine DNA methylfransferase inhibitors of the invention is diphenyl bonnic ester. Accordingly, several additional compounds based on this ester were prepared as follows. These compounds have the following structures: The general synthesis of these compounds is shown m Reaction Scheme 7. 1. Synthesis of bonnic acids (Compound 8). Dichloroborane dimethyl sulfide complex (0.5 - 2mL) was dissolved m either tefrahydrofuran or diethyl ether under argon and cooled to -78°C. he appropnate phenyl Gngnard reagent (2 molar equivalents), in tefrahydrofuran, diethyl ether, cyclohexane or mixtures of these solvents, was added dropwise to the cold reaction. The reaction was allowed to warm to room temperature and stirced overnight. Diethyl ether was added to the reaction and the reaction was hydrolyzed by the slow addition of IN hydrochlonc acid. The layers were separated and the organic layer was washed with saturated aqueous NaCl. The organic layer was dned over magnesium sulfate (MgS0 ), filtered and the solvent was removed in vacuo to give the crude product as a clear oil. This crude preparation of the title compound was used directly in the next stage of the synthesis Reaction Scheme 7 (10) where the reaction conditions are: i) tefrahydrofuran (THF) or ethyl ether (Et 2 0), -78 °C to room temperature overnight; n) EtOH, 8-hydroxyqumolme, room temperature; in) EtOH, 2-ammoethanol, room temperature. X can represent up to 5 substituents on each phenyl group, which can be independently hydrogen, lower alkyl, aryl or substituted aryl, lower alkoxy, lower alkoxyalkyl, or cycloalkyl or cycloalkyl alkoxy, where each cycloalkyl group has from 3-7 members, where up to two of the cycloalkyl members are optionally hetero atoms selected from oxygen and nitrogen, and where any member of the alkyl, aryl or cycloalkyl group is optionally substituted with halogen, lower alkyl or lower alkoxy, aryl or substituted aryl, halide, nitro, nitroso, aldehyde, carboxylic acid, esters, amides, or sulfates. 2. Synthesis of borimc acid 8-hydroxyqumolιne esters (Compounds 9). The crude bonmc acid (8) was dissolved in 0.05-5mL ethanol and was treated with 1-2 equivalents of IM 8-hydroxyqumolme m ethanol. The product either precipitated from the solution or the solution was concentrated and left to crystallize, once a solid had formed; the product was collected by filtration and washed with ethanol. 3. Synthesis of bonnic acid ethanolamme esters (Compounds 10). The crude bonnic acid (8) was dissolved in 0.05-5mL ethanol and treated with 1- 2 equivalents of IM ethanolamme in ethanol. The product either precipitated from the solution or the solution was concentrated and left to crystallize, once a solid had formed, the product was collected by filtration and washed with ethanol. The compounds (1) - (5) prepared as descnbed above were tested using the in vivo assays of the invention using Caulobacter cresentus, and compounds (1), (2), (4) and (5) have been tested for cell growth inhibition against Bacillus subtilis. The IC5 0 values are shown in Table II. TABLE II These compounds have advantageous physical properties, and are isolated as pure, stable solids that are amenable to large-scale production. Additional specific embodiments of adenine DNA methyltransferase inhibitors of the invention includes related compounds having these additional features: 1) Analogues with vanous substituents on the phenyl rings m any, or combination of, the ortho-, meta- and para- positions, including fused πngs and substituted fused nngs; 2) Analogues having aromatic heterocycles of various nng sizes, substituted heterocycles, fused heterocycles and substituted fused heterocycles in place of one or both phenyl groups; 3) Analogues having two non-identical aromatic rings bound to the boron atom, using combinations of the aromatic systems described m l) and 2) above; 4) Analogues prepared using qumolmes (9) containing various substituents in any possible position or structural analogues including fused heteroaromatic rings containing one or more heteroatom m any possible position or fused heteroaromatic rings containing one or more heteroatom m any possible position and containing various substituents in any possible position; and 5) Analogues having substitutions on either, or both of, the C-l and C-2 positions of the ethylene group of the 2-amιnoethanol of (10). It should be understood that the foregoing disclosure emphasizes certain specific embodiments of the invention and that all modifications or alternatives equivalent thereto are withm the spirit and scope of the invention as set forth in the appended claims.

Claims: WHAT WE CLAIM IS: 1. A compound of the formula or a pharmaceutically acceptable salt thereof, wherein R 1 , R 2 , and R 3 are the same or different and are independently hydrogen, lower alkyl, aryl or substituted aryl, lower alkoxy, lower alkoxyalkyl, or cycloalkyl or cycloalkyl alkoxy, where each cycloalkyl group has from 3- 7 members, where up to two of the cycloalkyl members are optionally hetero atoms selected from oxygen and nitrogen, and where any member of the alkyl, aryl or cycloalkyl group is optionally substituted with halogen, lower alkyl or lower alkoxy, aryl or substituted aryl, and where R can be nbose, deoxynbose or phosphorylated derivatives thereof, wherein R 1 , R 2 , and R 3 are not all hydrogen and wherein when R 3 is nbose, deoxynbose or phosphorylated derivatives thereof, one of R 1 or R 2 is not hydrogen. 2. A compound according to claim 1 , wherein R 1 and R 2 are the same or different and are independently hydrogen, histamine dihydrochlonde, norphenylephrme hydrochlonde, 1 ,2-dιammopropane, 5-ammo-l ,3,3-tnmethylcyclohexanemethyl-amme, 3-ιsopropoxypropylamme, diphenylbonmc acid, ethanolamme ester, 2-(2- ammoethylammo)-ethanol, tetrahydrofurfurylamme, 5-methyltryptamme hydrochlonde, 3 ,3 -d phenylpropylamme, 1 -(3-ammopropyl)-2-pynohdmone, 2-(2-ammoethyl)- 1 - methylpynohdme, 2-(ammomethyl)benzιmιdazole dihydro-chloπdehyrate, 2,2,2- tnfluoroethylamme, hydrochlonde, L-carnosme, (R)-(-)- 1 -ammo-2-propanol, 2-(l- cyclohexenyl)ethylamme, 4-(tπfluoromethyl)benzylamιne, 2,5-dιchloroamylamme hydrochlonde, (+/-)-4-amιno-3-hydroxybutync acid, N,N-dιmethylethylenedιamιne, 3,3- dimethylbutylamme, 1 ,4-dιammo-2-butanone dihydrochlonde, ammomethylbenzoic acid, ammohydroxymethylpropane diol, 2-(amιnoethyl)pyπdme, ammobutanol, adamantamme, am ohexanoic acid, N-benyzylethanolamme, ethyl-6-ammobutyrate, hydrochlonde, ethylenediamme, or 2-cyclohex-l-enylethylamme. 3. A compound according to claim 1 , wherein R is methyl 4-ιodobutyrate, l-bromo-3-phenylpropane, cmnamyl bromide, 2-chloroethylphosphonιc acid, ethyl 2-(2- chloroacetamιdo)-4-thιazole-acetate, 4-(2-chloroethyl)morpholιne hydrochlonde, (2- bromoethyl)tπmethylammonιum bromide, 4-chlorophenyl 2-bromoethyl ether, N-(3- bromopropyl)pthahmιde, 2-chloroethyl, isocyanate, 2-chloro-N, N- dimethylacetoacetamide, 3-chloro-2-hydroxypropyl methacrylate, 2-bromo-2' -hydroxy - 5'-nιtroacetanιhde, 3-(2-bromoethyl)ιndole, 5-chloro-2-pentanone ethylene ketal, 2- chloroethyl ethyl sulfide, 3-chloro-N-hydroxy-2,2-dιmethyl-propιonamιde, L-l-p- Tosylammo-2-Phenylethyl chloromethyl ketone, 2-(2-bromoethyl)-l,3-dιoxolane, ethyl 2-(bromomethyl)acrylate, 2-(2-(2-chlorethyoxy)ethoxy)ethanol, (3- chloropropyl)tπmethoxysιlane, chloramphenicol, 4-(chloromethyl)benzoιc acid, bromoethylamme hydrochlonde, epibromohydrm, lodopentane, or benzyl bromide 4. A compound according to claim 1, wherein R is H, R is (2- diphenylbormic ester) ethyl or diphenylpropyl, and R is H, 2-(4-morpholmyl)-ethyl, 3- (N-phthaloyl)-ammopropyl, 2-(2-(2-hydroxyethoxy)ethoxy)ethyl, or ethyl-2-(acrylate)- methyl. 5. A compound according to claim 1, wherein R is H, R is (S- homocystemyl)methyl and R 3 is nbose, 5 'phosphorylπbose, deoxynbose or 5' phosphoryl deoxynbose. 6. A compound according to claim 1, wherein R 3 is H and R 1 and R 2 are together 2-(dιphenylmethyl) cyclopentyl or 2-(dιphenylhydroxymethyl) cyclopentyl. 7. A compound according to claim 1, wherein R is H, R 2 is alanylbutyl ester, 2-alkylketone-2-ammoethyl, 2-ammoethyl or mono- or bisubstituted 2-ammo ethyl, and R 3 is 2-(4-morpholmyl)-ethyl. 8. A compound according to claim 1, wherein R 1 and R 2 are the same or different and are independently hydrogen, histamine dihydrochlonde, norphenylephnne hydrochlonde, 1 ,2-dιammopropane, 5-ammo-l ,3,3-tnmethylcyclohexanemethyl-amme, 3-ιsopropoxypropylamme, diphenylbonmc acid, ethanolamme ester, 2-(2- ammoethylammo)-ethanol, tefrahydrofurfurylamme, 5-methyltryptamme hydrochlonde, 3,3-dιphenylpropylamme, l-(3-amιnopropyl)-2-pynohdmone, 2-(2-ammoethyl)-l- methylpynohdme, 2-(ammomethyl)benzιmιdazole dihydro-chloπdehyrate, 2,2,2- tπfluoroethylamme, hydrochlonde, L-carnosme, (R)-(-)-l-ammo-2-propanol, 2-(l- cyclohexenyl)ethylamιne, 4-(trifluoromethyl)benzylamme, 2,5-dιchloroamylamme hydrochlonde, (+/-)-4-amιno-3-hydroxybutync acid, N,N-dιmethylethylenedιamme, 3,3- dimethylbutylamme, 1 ,4-dιammo-2-butanone dihydrochlonde, ammomethylbenzoic acid, ammohydroxymethylpropane diol, 2-(amιnoethyl)pyndme, ammobutanol, adamantamme, ammohexanoic acid, N-benyzylethanolamme, ethyl-6-ammobutyrate, hydrochlonde, ethylenediamme, or 2-cyclohex-l-enylethylamιne, and R 3 is methyl 4- lodobutyrate, l-bromo-3-phenylpropane, cmnamyl bromide, 2-chloroethylphosphomc acid, ethyl 2-(2-chloroacetamιdo)-4-thιazole-acetate, 4-(2-chloroethyl)morpholme hydrochlonde, (2-bromoethyl)tnmethylammomum bromide, 4-chlorophenyl 2- bromoethyl ether, N-(3-bromopropyl)pthahmιde, 2-chloroethyl, isocyanate, 2-chloro-N, N-dimethylacetoacetamide, 3-chloro-2-hydroxypropyl methacrylate, 2-bromo-2'- hydroxy-5'-nιtroacetanιlιde, 3-(2-bromoethyl)mdole, 5-chloro-2-pentanone ethylene ketal, 2-chloroethyl ethyl sulfide, 3-chloro-N-hydroxy-2,2-dιmethyl-propιonamιde, L-l- p-Tosylammo-2-Phenylethyl chloromethyl ketone, 2-(2-bromoefhyl)-l,3-dιoxolane, ethyl 2-(bromomethyl)acrylate, 2-(2-(2-chlorethyoxy)ethoxy)ethanol, (3- chloropropyl)tnmethoxysιlane, chloramphemcol, 4-(chloromethyl)benzoιc acid, bromoethylamme hydrochlonde, epibromohydnn, lodopentane, or benzyl bromide. 9. A compound according to claim 1, selected from 6-N-(dιphenylbonnιc ester)-ethyl-adenme, 6-N-(dιphenylboπmc ester)-ethyl-9-(2-(4-morpholmyl)-ethyl)- adenme, 6-N-(dιphenylbonnιc ester)-ethyl-9-(3-(N-phthaloyl)-ammopropyl)-adenme, 6- N-(dιphenylboπmc ester)-ethyl-9-(2-(2-(2-hydroxyethoxy)ethoxy)ethyl)-adenme, and 6- N-(dιphenylbonnιc ester)-ethyl-9-(ethyl-2-acrylate)-methyl-adenme. 10. A pharmaceutical composition compnsmg a compound according to Claim 1 combined with at least one pharmaceutically acceptable earner or excipient. 11 A method for the treatment of a disease or disorder associated with infection with a pathogenic bacteria that expresses an adenine DNA methyltransferase, said method compnsmg administering to a patient in need of such treatment a therapeutically-effective amount of a compound of claim 1. 12. A method according to Claim 11 wherein the disease or disorder associated infection with a pathogenic bacteria is Staphylococcus aureus; Staphylococcus saprophyticus; Streptococcus pyrogenes, Streptococcus agalactiae, Streptococcus pneumomae; Bacillus anthracis; Corynebacterium diphtheria; Clostridium perfringens; Clostridium botuhnum, Clostridium tetani; Neissena gonorrhoeae, Neissena meningitidis; Pseudomonas aeruginosa, Legwnella pneumophύa, Escherichia coh, Yersinia pestis; Hemophilus influenzae; Hehcobacter pylori, Campylobacter fetus; Vibrio cholerae; Vibrio parahemolyticus; Trepomena palhdum, Actinomyces israelu; Rickettsia prowazekii; Rickettsia rickettsii; Chlamydia trachomatis, Chlamydia psittaci; Brucella abortus or Agrobactenum tumefaciens 13. A compound of the formula or a pharmaceutically acceptable salt thereof, wherein R a , R b , and R c are the same or different and are independently hydrogen, halogen, nitro, nitroso, lower alkyl, aryl or substituted aryl, lower alkoxy, lower alkoxyalkyl, or cycloalkyl or cycloalkyl alkoxy, where each cycloalkyl group has from 3-7 members, where up to two of the cycloalkyl members are optionally hetero atoms selected from sulfur, oxygen and nitrogen, and where any member of the alkyl, aryl or cycloalkyl group is optionally substituted with halogen, lower alkyl or lower alkoxy, aryl or substituted aryl, halogen, nitro, nitroso, aldehyde, carboxyhc acid, amide, ester, or sulfate, or wherein R a , R b and R c may be connected by aromatic, aliphatic, heteroaromatic, heteroahphatic ring structures or substituted embodiments thereof, and wherein Ar and Ar can be the same or different and are each independently aryl or aryl substituted at one or a plurality of positions with halogen, nitro, nitroso, lower alkyl, aryl or substituted aryl, lower alkoxy, lower alkoxyalkyl, or cycloalkyl or cycloalkyl alkoxy, where each cycloalkyl group has from 3-7 members, where up to two of the cycloalkyl members are optionally hetero atoms selected from sulfur, oxygen and nitrogen, and where any member of the alkyl, aryl or cycloalkyl group is optionally substituted with halogen, lower alkyl or lower alkoxy, aryl or substituted aryl, halogen, nitro, nitroso, aldehyde, carboxyhc acid, amide, ester, or sulfate, and wherein bond 1 and bond 2 are independently a single bond or a double bond. 14. A compound according to claim 14, selected from dι-(p- fluorophenyl)bonnιc acid 8- hydroxyqumilme ester, dι-(p-chlorophenyl)bonnιc acid 8- hydroxyquimlme ester, diphenylbonmc acid 8-hydroxyqumιlme ester, dι-(p- fluorophenyl)bonmc acid ethanolamme ester, and dι-(p-chlorophenyl)boπmc acid ethanolamme ester. 15. A pharmaceutical composition comprising a compound according to Claim 1 combined with at least one pharmaceutically acceptable earner or excipient. 16. A method for the treatment of a disease or disorder associated with infection with a pathogenic bacteria that expresses an adenine DNA methyltransferase, said method compnsmg admmistenng to a patient in need of such treatment a therapeutically-effective amount of a compound of claim 1. 17. A method according to Claim 11 wherein the disease or disorder associated infection with a pathogenic bactena is Staphylococcus aureus; Staphylococcus saprophyticus; Streptococcus pyrogenes; Streptococcus agalactiae; Streptococcus pneumoniae; Bacillus anthracis; Corynebacterium diphtheria; Clostridium perfringens; Clostridium botulinum; Clostridium tetani; Neissena gonorrhoeae; Neissena meningitidis; Pseudomonas aeruginosa; Legionella pneumophila; Escherichia coli; Yersinia pestis; Hemophilus influenzae; Hehcobacter pylori; Campylobacter fetus; Vibrio cholerae; Vibrio parahemolyticus; Trepomena pallidum; Actinomyces israehi; Rickettsia prowazekii; Rickettsia rickettsii; Chlamydia trachomatis; Chlamydia psittaci; Brucella abortus or Agrobactenum tumefaciens. 18. A combinatorial library compnsmg a multiplicity of compounds according to claim 1. 19. A combinatorial library comprising a multiplicity of compounds according to claim 13. 20. A packaged pharmaceutical composition compnsmg the pharmaceutical composition of Claim 10 m a container and instructions for using the composition to treat a patient suffenng from a disease or disorder associated with infection with a pathogenic bactena.

Inventors: BAKER STEPHEN J, BENKOVIC STEPHEN J, SHAPIRO LUCILLE, WAHNON DAPHNE C, WALL MARK, BAKER, STEPHEN, J., BENKOVIC, STEPHEN, J., SHAPIRO, LUCILLE, WAHNON, DAPHNE, C., WALL, MARK
Applicants: BAKER STEPHEN J, BENKOVIC STEPHEN J, PENN STATE RES FOUND, SHAPIRO LUCILLE, WAHNON DAPHNE C, WALL MARK, BAKER, STEPHEN, J., BENKOVIC, STEPHEN, J., SHAPIRO, LUCILLE, THE PENN STATE RESEARCH FOUNDATION, WAHNON, DAPHNE, C., WALL, MARK
Application year: 2000
Main classification: C07D473
IPC codes: A61K31, A61P31, A61P43, C07D473, C07F5, C07H19, C12N9