PROCESS FOR THE PREPARATION OF 7-AZABICYCLO (4.1.0)-HEPT-3-ENE-3-CARBOXYLIC ACID ESTERS

Abstract: The present invention provides a method of producing 7-azabicyclo[4.1.0]hept-3-ene-3-carboxylic acid ester (VII) economically, industrially advantageously and efficiently in a large amount. The present invention relates to a production method of compound (VII), which includes reacting epoxide (I) with amine (II) to give aminodiol (III), reacting the aminodiol (III) with a sulfonylating agent in the presence of a base to give azabicyclohept-2-ene (IV), reacting the azabicyclohept-2-ene (IV) with alcohol (V) in the presence of a Lewis acid to give azabicyclohept-3-ene (VI), and eliminating the 7-position substituent R 2 from the azabicyclohept-3-ene (VI).

Description: TECHNICAL FIELD The present invention relates to a production method of 7-azabicyclo[4.1.0]hept-3-ene-3-carboxylic acid ester. 7-Azabicyclo[4.1.0]hept-3-ene-3-carboxylic acid ester, such as ethyl (1α,5α,6α)-5-(1-ethylpropoxy)-7-azabicyclo[4.1.0]hept-3-ene-3-carboxylate, obtained by the present invention, is useful as a synthetic intermediate for GS4104 represented by the following formula. GS4104 is a compound under development as a novel agent for the prophylaxis and treatment of influenza based on the action to prevent viral growth by inhibiting neuraminidase present on the surface of influenza virus (hereinafter to be generally referred to as anti-influenza drug) [see Journal of Organic Chemistry (J. Org. Chem.), vol. 63, p. 4545 (1998); Journal of American Chemical Society (J. Am. Chem. Soc.), vol. 119, p. 681 (1997)]. BACKGROUND ART As a conventional synthetic method of 7-azabicyclo[4.1.0]hept-3-ene-3-carboxylic acid ester, such as ethyl (1α,5α,6α)-5-(1-ethylpropoxy)-7-azabicyclo[4.1.0]hept-3-ene-3-carboxylate, a synthetic method wherein shikimic acid is used as a starting material [see Journal of Organic Chemistry (J. Org. Chem.), vol. 63, p. 4545 (1998); WO 99/14185; WO 98/07685], and a synthetic method wherein quinic acid is used as a starting material [see Journal of American Chemical Society (J. Am. Chem. Soc.), vol. 119, p. 681 (1997)] and the like are known. The shikimic acid and quinic acid used as a starting material for the conventional synthetic method of ethyl (1α,5α,6α)-5-(1-ethylpropoxy)-7-azabicyclo[4.1.0]hept-3-ene-3-carboxylate are produced in less amounts and expensive. All the above-mentioned methods require many reaction steps. As widely known, influenza often becomes an epidemic disease worldwide, and an anti-influenza drug is required to be economical and supplied in a large amount. The above-mentioned production methods are not necessarily advantageous as a production method of the intermediate for GS4104 under development as an anti-influenza drug from the industrial viewpoint, and there is a demand for a synthetic method capable of economical production in a large amount. DISCLOSURE OF INVENTION It is therefore an object of the present invention to provide a production method of a 7-azabicyclo[4.1.0]hept-3-ene-3-carboxylic acid ester, such as ethyl (1α,5α,6α)-5-(1-ethylpropoxy)-7-azabicyclo[4.1.0]hept-3-ene-3-carboxylate, useful as a synthetic intermediate for GS4104 under development as an anti-influenza drug, economically, industrially advantageously and efficiently in a large amount. According to the present invention, the above-mentioned objects can be achieved by providing (1) a production method of a 7-azabicyclo[4.1.0]hept-3-ene-3-carboxylic acid ester of the formula (VII) wherein R 1 is C 1-10 alkyl group which may have any substituent selected from C 1-10 alkoxyl group, halogen atom, cyano group and nitro group; or C 3-8 cycloalkyl group, C 6-10 aryl group or aralkyl group wherein the alkyl moiety is C 1-6 alkyl group and the aryl moiety has 1 to 3 C 6-10 aryl groups, where said cycloalkyl, aryl and aralkyl groups may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group and R 3 is C 1-10 alkyl group which may have any substituent selected from C 1-10 alkoxyl group, halogen atom, cyano group and nitro group; or C 3-8 cycloalkyl group, C 6-10 aryl group or aralkyl group wherein the alkyl moiety is C 1-6 alkyl group and the aryl moiety has 1 to 3 C 6-10 aryl groups, where said cycloalkyl, aryl and aralkyl groups may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group; or C 2-10 alkenyl group which may have any substituent selected from C 1-6 alkoxyl group, halogen atom, cyano group and nitro group, which comprises the steps of (A) reacting a 5-hydroxy-7-oxabicyclo[4.1.0]hept-2-ene-3-carboxylic acid ester of the formula (I) wherein R 1 is as defined above, with an amine of the formula (II) R 2 NH 2 wherein R 2 is a hydrogen atom, or C 1-10 alkyl group which may have any substituent selected from C 1-10 alkoxyl group, halogen atom, cyano group and nitro group; or C 3-8 cycloalkyl group, C 6-10 aryl group or aralkyl group wherein the alkyl moiety is C 1-6 alkyl group and the aryl moiety has 1 to 3 C 6-10 aryl groups, where said cycloalkyl, aryl and aralkyl groups may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group; or C 2-10 alkenyl group which may have any substituent selected from C 1-6 alkoxyl group, halogen atom, cyano group and nitro group, to give a 3-amino-4,5-dihydroxy-1-cyclohexene-1-carboxylic acid ester of the formula (III) wherein R 1 and R 2 are as defined above, (B) reacting the obtained 3-amino-4,5-dihydroxy-1-cyclohexene-1-carboxylic acid ester with a sulfonylating agent in the presence of a base to give a 5-sulfonyloxy-7-azabicyclo[4.1.0]hept-2-ene-3-carboxylic acid ester of the formula (IV) wherein R 1 and R 2 are as defined above and A is alkylsulfonyl group wherein the alkyl moiety is C 1-6 alkyl group, which may have any substituent selected from C 1-6 alkoxyl group, halogen atom, cyano group and nitro group; phenylsulfonyl group which may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group on the aromatic ring; or aralkylsulfonyl group wherein the aryl moiety is phenyl group and the alkyl moiety is C 1-6 alkyl group, which may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group on the aromatic ring, (C) reacting the obtained 5-sulfonyloxy-7-azabicyclo[4.1.0]hept-2-ene-3-carboxylic acid ester with an alcohol of the formula (V) R 3 OH wherein R 3 is as defined above, in the presence of a Lewis acid to give a 5-oxy-7-azabicyclo[4.1.0]hept-3-ene-3-carboxylic acid ester of the formula (VI) wherein R 1 , R 2 and R 3 are as defined above, and (D) when R 2 is a C 1-10 alkyl group which may have any substituent selected from C 1-10 alkoxyl group, halogen atom, cyano group and nitro group; or C 3-8 cycloalkyl group, C 6-10 aryl group or aralkyl group wherein the alkyl moiety is C 1-6 alkyl group and the aryl moiety has 1 to 3 C 6-10 aryl groups, where said cycloalkyl, aryl and aralkyl groups may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group; or C 2-10 alkenyl group which may have any substituent selected from C 1-6 alkoxyl group, halogen atom, cyano group and nitro group, eliminating the 7-position substituent R 2 of the obtained 5-oxy-7-azabicyclo[4.1.0]hept-3-ene-3-carboxylic acid ester (VI). 2. A production method of a 7-azabicyclo[4.1.0]hept-3-ene-3-carboxylic acid ester of the formula (VII) wherein R 1 is C 1-10 alkyl group which may have any substituent selected from C 1-10 alkoxyl group, halogen atom, cyano group and nitro group; or C 3-8 cycloalkyl group, C 6-10 aryl group or aralkyl group wherein the alkyl moiety is C 1-6 alkyl group and the aryl moiety has 1 to 3 C 6-10 aryl groups, where said cycloalkyl, aryl and aralkyl groups may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group and R 3 is C 1-10 alkyl group which may have any substituent selected from C 1-10 alkoxyl group, halogen atom, cyano group and nitro group; or C 3-8 cycloalkyl group, C 6-10 aryl group or aralkyl group wherein the alkyl moiety is C 1-6 alkyl group and the aryl moiety has 1 to 3 C 6-10 aryl groups, where said cycloalkyl, aryl and aralkyl groups may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group; or C 2-10 alkenyl group which may have any substituent selected from C 1-6 alkoxyl group, halogen atom, cyano group and nitro group, which comprises the steps of (A) reacting a 5-hydroxy-7-oxabicyclo[4.1.0]hept-2-ene-3-carboxylic acid ester of the formula (I) wherein R 1 is as defined above, with an amine of the formula (II) R 2 NH 2 wherein R 2 is a hydrogen atom, or C 1-10 alkyl group which may have any substituent selected from C 1-10 alkoxyl group, halogen atom, cyano group and nitro group; or C 3-8 cycloalkyl group, C 6-10 aryl group or aralkyl group wherein the alkyl moiety is C 1-6 alkyl group and the aryl moiety has 1 to 3 C 6-10 aryl groups, where said cycloalkyl, aryl and aralkyl groups may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group; or C 2-10 alkenyl group which may have any substituent selected from C 1-6 alkoxyl group, halogen atom, cyano group and nitro group, to give a 3-amino-4,5-dihydroxy-1-cyclohexene-1-carboxylic acid ester of the formula (III) wherein R 1 and R 2 are as defined above, (B) protecting an amino group of the obtained 3-amino-4,5-dihydroxy-1-cyclohexene-1-carboxylic acid ester to give a 3-amino-4,5-dihydroxy-1-cyclohexene-1-carboxylic acid ester of the formula (VIII) wherein R 1 and R 2 are as defined above and R 4 is amino-protecting group selected from alkoxycarbonyl group, aralkyloxycarbonyl group, aralkyl group and acyl group, (C) reacting the obtained 3-amino-4,5-dihydroxy-1-cyclohexene-1-carboxylic acid ester with a sulfonylating agent in the presence of a base to give a 3-amino-4,5-disulfonyloxy-1-cyclohexene-1-carboxylic acid ester of the formula (IX) wherein R 1 , R 2 and R 4 are as defined above and A is alkylsulfonyl group wherein the alkyl moiety is C 1-6 alkyl group, which may have any substituent selected from C 1-6 alkoxyl group, halogen atom, cyano group and nitro group; phenylsulfonyl group which may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group on the aromatic ring; or aralkylsulfonyl group wherein the aryl moiety is phenyl group and the alkyl moiety is C 1-6 alkyl group, which may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group on the aromatic ring, (D) removing the amino-protecting group from the obtained 3-amino-4,5-disulfonyloxy-1-cyclohexene-1-carboxylic acid ester to give a 3-amino-4,5-disulfonyloxy-1-cyclohexene-1-carboxylic acid ester of the formula (X) wherein R 1 , R 2 and A are as defined above, (E) reacting the obtained 3-amino-4,5-disulfonyloxy-1-cyclohexene-1-carboxylic acid ester with a base to give a 5-sulfonyloxy-7-azabicyclo[4.1.0]hept-2-ene-3-carboxylic acid ester of the formula (IV) wherein R 1 , R 2 and A are as defined above, (F) reacting the obtained 5-sulfonyloxy-7-azabicyclo[4.1.0]hept-2-ene-3-carboxylic acid ester with an alcohol of the formula (V) R 3 OH wherein R 3 is as defined above, in the presence of a Lewis acid to give a 5-oxy-7-azabicyclo[4.1.0]hept-3-ene-3-carboxylic acid ester of the formula (VI) wherein R 1 , R 2 and R 3 are as defined above, and (G) when R 2 is a C 1-10 alkyl group which may have any substituent selected from C 1-10 alkoxyl group, halogen atom, cyano group and nitro group; or C 3-8 cycloalkyl group, C 6-10 aryl group or aralkyl group wherein the alkyl moiety is C 1-6 alkyl group and the aryl moiety has 1 to 3 C 6-10 aryl groups, where said cycloalkyl, aryl and aralkyl groups may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group; or C 2-10 alkenyl group which may have any substituent selected from C 1-6 alkoxyl group, halogen atom, cyano group and nitro group, eliminating the 7-position substituent R 2 from the obtained 5-oxy-7-azabicyclo[9.1.0]hept-3-ene-3-carboxylic acid ester (VI). 3. A production method of a 5-oxy-7-azabicyclo[4.1.0]hept-3-ene-3-carboxylic acid ester of the formula (VI) wherein R 1 is C 1-10 alkyl group which may have any substituent selected from C 1-10 alkoxyl group, halogen atom, cyano group and nitro group; or C 3-8 cycloalkyl group, C 6-10 aryl group or aralkyl group wherein the alkyl moiety is C 1-6 alkyl group and the aryl moiety has 1 to 3 C 6-10 aryl groups, where said cycloalkyl, aryl and aralkyl groups may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group, R 2 is a hydrogen atom, or C 1-10 alkyl group which may have any substituent selected from C 1-10 alkoxyl group, halogen atom, cyano group and nitro group; or C 3-8 cycloalkyl group, C 6-10 aryl group or aralkyl group wherein the alkyl moiety is C 1-6 alkyl group and the aryl moiety has 1 to 3 C 6-10 aryl groups, where said cycloalkyl, aryl and aralkyl groups may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group; or C 2-10 alkenyl group which may have any substituent selected from C 1-6 alkoxyl group, halogen atom, cyano group and nitro group and R 3 is C 1-10 alkyl group which may have any substituent selected from C 1-10 alkoxyl group, halogen atom, cyano group and nitro group; or C 3-8 cycloalkyl group, C 6-10 aryl group or aralkyl group wherein the alkyl moiety is C 1-6 alkyl group and the aryl moiety has 1 to 3 C 6-10 aryl groups, where said cycloalkyl, aryl and aralkyl groups may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group; or C 2-10 alkenyl group which may have any substituent selected from C 1-6 alkoxyl group, halogen atom, cyano group and nitro group, which comprises reacting a 5-sulfonyloxy-7-azabicyclo[4.1.0]hept-2-ene-3-carboxylic acid ester of the formula (IV) wherein A is alkylsulfonyl group wherein the alkyl moiety is C 1-6 alkyl group, which may have any substituent selected from C 1-6 alkoxyl group, halogen atom, cyano group and nitro group; phenylsulfonyl group which may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group on the aromatic ring; or aralkylsulfonyl group wherein the aryl moiety is phenyl group and the alkyl moiety is C 1-6 alkyl group, which may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group on the aromatic ring, and R 1 and R 2 are as defined above, with an alcohol of the formula (V) R 3 OH wherein R 3 is as defined above, in the presence of a Lewis acid. In the above-mentioned formulas, the alkyl group represented by R 1 , R 2 and R 3 is a straight chain or branched chain alkyl group preferably having 1 to 10, more preferably 1 to 6 carbon atoms. Examples thereof include methyl group, ethyl group, propyl group, isopropyl group, n-butyl group, isobutyl group, tert-butyl group, pentyl group, 1-ethylpropyl group, hexyl group. These alkyl groups may have substituents and examples of the substituent include alkoxyl group preferably having 1 to 10, more preferably 1 to 6 carbon atoms, such as methoxy group, ethoxy group, propoxy group, butoxy group; halogen atom such as fluorine atom, chlorine atom, bromine atom; cyano group; nitro group. The cycloalkyl group represented by R 1 , R 2 and R 3 is cycloalkyl group preferably having 3 to 8 carbon atoms and is exemplified by cyclopentyl group, cyclohexyl group, cyclooctyl group. The aryl group represented by R 1 , R 2 and R 3 is aryl group preferably having 6 to 10 carbon atoms, and is exemplified by phenyl group, naphthyl group. The aralkyl group represented by R 1 , R 2 and R 3 is aralkyl group wherein the alkyl moiety is alkyl group preferably having 1 to 6 carbon atoms and the aryl moiety has 1 to 3 aryl groups defined above. Examples thereof include benzyl group, diphenylmethyl group, triphenylmethyl group, phenethyl group. These cycloalkyl group, aryl group and aralkyl group may have substituents and examples of the substituent include alkyl group preferably having 1 to 6 carbon atoms such as methyl group, ethyl group, propyl group, isopropyl group, n-butyl group, isobutyl group, tert-butyl group; alkoxyl group preferably having 1 to 6 carbon atoms such as methoxy group, ethoxy group, propoxy group, butoxy group; halogen atom such as fluorine atom, chlorine atom, bromine atom; cyano group; nitro group. The alkenyl group represented by R 2 and R 3 is a straight chain or branched chain alkenyl group preferably having 2 to 10, more preferably 2 to 6 carbon atoms. Examples thereof include allyl group, isopropenyl group, 2-methylallyl group. These alkenyl groups may have substituents and examples of the substituent include alkoxyl group preferably having 1 to 6 carbon atoms such as methoxy group, ethoxy group, propoxy group, butoxy group; halogen atom such as fluorine atom, chlorine atom, bromine atom; cyano group; nitro group. As R 2 , an aralkyl group optionally having substituents (e.g., benzyl group, diphenylmethyl group) is preferable. The protecting group of the amino group represented by R 4 is free of any particular limitation as long as it is generally used for protecting the amino group, and is exemplified by alkoxycarbonyl group such as tert-butoxycarbonyl group, 2,2,2-trichloroethoxycarbonyl group, 2-iodoethoxycarbonyl group, 2-trimethylsilylethoxycarbonyl group, 2-methylthioethoxycarbonyl group, 1,1-dimethylpropyloxycarbonyl group, 1-methyl-1-phenylethoxycarbonyl group, vinyloxycarbonyl group, allyloxycarbonyl group, cinnamyloxycarbonyl group, 1,1-dimethylpropynyloxycarbonyl group; aralkyloxycarbonyl group such as 9-fluorenylmethoxycarbonyl group, benzyloxycarbonyl group, p-methoxybenzyloxycarbonyl group, 3,5-dimethoxybenzyloxycarbonyl group, p-nitrobenzyloxycarbonyl group, o-nitrobenzyloxycarbonyl group; aralkyl group such as benzyl group, 2,4,6-trimethylbenzyl group, p-methoxybenzyl group, 3,5-dimethoxybenzyl group, p-nitrobenzyl group, o-nitrobenzyl group, o-chlorobenzyl group, p-chlorobenzyl group, o-bromobenzyl group, p-bromobenzyl group, 2,4-dichlorobenzyl group, p-cyanobenzyl group, m-chloro-p-acyloxybenzyl group, 9-anthrylmethyl group, diphenylmethyl group, phenyl(o-nitrophenyl)methyl group, di(2-pyridyl)methyl group, (4-pyridyl)methyl group, triphenylmethyl group; acyl group such as formyl group, acetyl group, chloroacetyl group, dichloroacetyl group, trichloroacetyl group, trifluoroacetyl group, o-nitrophenylacetyl group, p-nitrophenylacetyl group, o-nitrophenoxyacetyl group, p-nitrophenoxyacetyl group, acetoacetyl group, pyridylacetyl group. The organic sulfonyl group represented by A is free of any particular limitation as long as it is bonded with an organic group, and is exemplified by alkylsulfonyl group optionally having substituents, arylsulfonyl group optionally having substituents, aralkylsulfonyl group optionally having substituents. The alkyl moiety of the alkylsulfonyl group is alkyl group preferably having 1 to 6 carbon atoms. The aryl moiety of the arylsulfonyl group is, for example, phenyl group. The aryl moiety of the aralkylsulfonyl group is, for example, phenyl group, and the alkyl moiety is alkyl group preferably having 1 to 6 carbon atoms. The alkylsulfonyl group may have substituents, and examples of the substituent include alkoxyl group (alkoxyl group preferably having 1 to 6 carbon atoms), halogen atom, cyano group, nitro group. The arylsulfonyl group and aralkylsulfonyl group may have substituents on the aromatic ring, and examples of the substituent include alkyl group (alkyl group preferably having 1 to 6 carbon atoms), alkoxyl group (alkoxyl group preferably having 1 to 6 carbon atoms), halogen atom, cyano group, nitro group. The organic sulfonyl group represented by A is exemplified by methanesulfonyl group, ethanesulfonyl group, benzenesulfonyl group, toluenesulfonyl group, p-methoxybenzenesulfonyl group, 2,4,6-trimethylbenzenesulfonyl group, benzylsulfonyl group, p-methylbenzylsulfonyl group, trifluoromethanesulfonyl group. The production method of 7-azabicyclo[4.1.0]hept-3-ene-3-carboxylic acid ester (VII) of the present invention is shown in the following scheme, and includes two production methods of route (i) and route (ii), which can be determined as appropriate according to the kind of amine (II) to be used. wherein R 1 , R 2 , R 3 , R 4 and A are as defined above. In the following, each step is explained. (a): step for reacting epoxide (I) with amine (II) to give aminodiol (III) Examples of the amine (II) include ammonia, methylamine, ethylamine, propylamine, n-butylamine, cyclohexylamine, cyclooctylamine, allylamine, isopropenylamine, aniline, 4-methylphenylamine, benzylamine, benzhydrylamine. Of these, benzylamine and benzhydrylamine are particularly preferable. The amount of the amine (II) to be used is generally preferably 1 to 10-fold moles, particularly preferably 1 to 1.5-fold moles, per 1 mole of epoxide (I). The reaction can be carried out in the presence or absence of a solvent. The solvent to be used is free of any particular limitation as long as it does not adversely affect the reaction. Examples thereof include aliphatic hydrocarbon such as hexane, heptane, octane and the like; aromatic hydrocarbon such as benzene, toluene, xylene, mesitylene and the like; ether such as diethyl ether, diisopropyl ether, tetrahydrofuran, dioxane and the like. When a solvent is used, the amount of the solvent to be used is free of any particular limitation, but it is generally preferably 1 to 10-fold weight, more preferably 1 to 3-fold weight, relative to epoxide (I). The reaction temperature is preferably 20 to 200°C, more preferably 50°C to 80°C. While the reaction time varies depending on the kind and the amount of epoxide (I), amine (II) and the solvent, it is generally within the range of 1 to 48 hours. The reaction is carried out by, for example, mixing epoxide (I), amine (II) and a solvent as necessary and stirring the mixture at a given temperature. The aminodiol (III) thus obtained can be purified and separated according to a method generally employed for the purification and separation of organic compounds. For example, the reaction mixture is concentrated, and the obtained concentrate is purified by distillation, column chromatography. (b): Step for reacting aminodiol (III) with a sulfonylating agent in the presence of a base to give azabicyclohept-2-ene (IV) Examples of the base include tertiary amine such as trimethylamine, triethylamine, tripropylamine, tributylamine, trioctylamine, pyridine, collidine, lutidine; alkaline metal hydride such as sodium hydride, potassium hydride; alkaline metal carbonate such as sodium carbonate, potassium carbonate. Of these, triethylamine, pyridine and lutidine are preferable. The amount of the base to be used is preferably 2 to 100-fold moles, more preferably 2 to 25-fold moles, per 1 mole of aminodiol (III). Examples of the sulfonylating agent include organic sulfonyl halide such as methanesulfonyl chloride, methanesulfonyl fluoride, ethanesulfonyl chloride, ethanesulfonyl bromide, benzenesulfonyl chloride, benzenesulfonyl bromide, benzenesulfonyl fluoride, toluenesulfonyl chloride, toluenesulfonyl bromide, toluenesulfonyl fluoride, p-methoxybenzenesulfonyl chloride, 2,4,6-trimethylbenzenesulfonyl chloride, benzylsulfonyl chloride, p-methylbenzylsulfonyl chloride, trifluoromethanesulfonyl chloride; sulfonic anhydride such as methanesulfonic anhydride, p-toluenesulfonic anhydride, trifluoromethanesulfonic anhydride. The amount of the sulfonylating agent to be used is preferably 2 to 10-fold moles, more preferably 2 to 5-fold moles, per 1 mole of aminodiol (III). The reaction is preferably carried out in a solvent. The solvent to be used is free of any particular limitation as long as it does not adversely affect the reaction. Examples thereof include aliphatic hydrocarbon such as hexane, heptane, octane; aromatic hydrocarbon such as benzene, toluene, xylene, mesitylene; ether such as diethyl ether, diisopropyl ether, tetrahydrofuran, dioxane; halogenated hydrocarbon such as dichloromethane, chloroform, carbon tetrachloride, 1,2-dichloroethane. The amount of the solvent to be used is free of any particular limitation, but it is generally preferably 1 to 100-fold weight, more preferably 1 to 10-fold weight, relative to aminodiol (III). The reaction temperature is preferably -20°C to 150°C, more preferably 10°C to 80°C. While the reaction time varies depending on the kind and the amount of aminodiol (III), base, sulfonylating agent and solvent, it is generally within the range of 1 to 48 hours. The reaction is carried out by, for example, dissolving aminodiol (III) and a base in a solvent, adding a sulfonylating agent and stirring the mixture at a given temperature. The azabicyclohept-2-ene (IV) thus obtained can be purified and separated according to a method generally employed for the purification and separation of organic compounds. For example, the reaction mixture is poured into water, extracted with aliphatic hydrocarbon such as n-hexane, aromatic hydrocarbon such as toluene, halogenated hydrocarbon such as dichloromethane, ether such as diethyl ether, diisopropyl ether and the extract is concentrated and the obtained concentrate is purified by distillation, column chromatography. (c): step for reacting azabicyclohept-2-ene (IV) and alcohol (V) in the presence of Lewis acid to give azabicyclohept-3-ene (VI) Examples of the alcohol (V) include primary alcohol such as methanol, ethanol, 1-propanol, 1-octanol, allyl alcohol, benzyl alcohol; secondary alcohol such as isopropanol, 2-butanol, 3-pentanol, cyclopentanol, cyclohexanol. Of these, the use of 3-pentanol as alcohol (V) is particularly preferable from the viewpoint of synthesis of ethyl (1α,5α,6α)-5-(1-ethylpropoxy)-7-azabicyclo[4.1.0]hept-3-ene-3-carboxylate, which is a synthetic intermediate for GS4104. The amount of the alcohol (V) to be used is preferably 1 to 200-fold moles, more preferably 10 to 100-fold moles, per 1 mole of azabicyclohept-2-ene (IV). Examples of the Lewis acid include boron trifluoride-etherate, aluminum chloride, zinc chloride, zinc iodide, titanium tetrachloride. The amount of the Lewis acid to be used is preferably 0.1 to 30-fold moles, more preferably 1 to 10-fold moles, per 1 mole of azabicyclohept-2-ene (IV). The reaction can be carried out in the presence or absence of a solvent. Examples of the solvent to be used is free of any particular limitation as long as it does not adversely affect the reaction. Examples of the solvent include aliphatic hydrocarbon such as hexane, heptane, octane; aromatic hydrocarbon such as benzene, toluene, xylene, mesitylene ; ether such as diethyl ether, diisopropyl ether, tetrahydrofuran, dioxane; halogenated hydrocarbon such as dichloromethane, chloroform, carbon tetrachloride, 1,2-dichloroethane. When a solvent is used, the amount thereof is free of any particular limitation. It is generally preferably 1 to 100-fold weight, more preferably 1 to 10-fold weight relative to azabicyclohept-2-ene (IV). For efficient progress of the reaction, a base may be also present during the reaction. Examples of the base include amine such as triethylamine, pyridine, collidine, lutidine; alkaline metal hydride such as sodium hydride, potassium hydride; alkaline metal carbonate such as sodium carbonate, potassium carbonate. When a base is co-used, the amount thereof is free of any particular limitation, but it is preferably 2 to 100-fold moles, more preferably 2 to 25-fold moles, relative to azabicyclohept-2-ene (IV). The reaction temperature is preferably 0-100°C, more preferably 10-80°C. While the reaction time varies depending on the kind and the amount of azabicyclohept-2-ene (IV), alcohol (V), Lewis acid and solvent, it is generally 0.5 to 10 hours. The reaction is carried out by, for example, mixing azabicyclohept-2-ene (IV), alcohol (V), Lewis acid and a solvent as necessary and stirring the mixture at a given temperature. When a base is further co-used for the reaction, the base is preferably added after start of stirring as mentioned above. In this case, the given amount of the base may be added at once or added by several-divided portions. The azabicyclohept-3-ene (VI) thus obtained can be purified and separated according to a method generally employed for the purification and separation of organic compounds. For example, the reaction mixture is poured into water, extracted with aliphatic hydrocarbon such as n-hexane, aromatic hydrocarbon such as toluene, ether such as diethyl ether, diisopropyl ether, and the extract is concentrated and the obtained concentrate is purified by distillation, column chromatography . When R 2 is a hydrogen atom, the objective 7-azabicyclo[4.1.0]hept-3-ene-3-carboxylic acid ester (VII) can be obtained at this stage without performing the next step (d). (d): step for obtaining 7-azabicyclo[4.1.0]hept-3-ene-3-carboxylic acid ester (VII) by eliminating the 7-position substituent R 2 from azabicyclohept-2-ene (VI) The 7-position substituent R 2 can be eliminated from azabicyclohept-3-ene (VI) by, for example, a treatment with an acid or according to a known method using catalytic hydrogenation (T. W. Green, "Protective Groups in Organic Synthesis," John-Wiley & Sons, New York, pp 218-287 (1981)). For the treatment with an acid, azabicyclohept-3-ene (VI) is brought into contact with, for example, a solution of hydrogen chloride or hydrogen bromide in alcohol such as ethanol . Examples of the catalytic hydrogenation include a method including reacting azabicyclohept-3-ene (VI) with a hydrogen source such as hydrogen, formic acid in the presence of a catalyst such as Raney-nickel, palladium-carbon. Particularly, when the 7-position substituent R 2 is an aralkyl group, such as benzyl group and diphenylmethyl group, a method for eliminating the 7-position substituent using palladium-carbon as a catalyst and formic acid as a hydrogen source is particularly preferable, because only the 7-position substituent can be selectively eliminated without reducing the double bond of cyclohexene ring. The palladium-carbon to be used here may be those commercially available for hydrogenation, where no limitation is imposed on the amount of palladium to be carried. The weight of palladium-carbon to be used is preferably 0.1 to 10-fold weight, more preferably 0.5 to 3-fold weight, relative to azabicyclohept-3-ene (VI). The amount of the formic acid to be used is preferably 1 to 100-fold moles, more preferably 1 to 10-fold moles, relative to azabicyclohept-3-ene (VI). The reaction is preferably carried out in a solvent. As the solvent, alcohol such as methanol, ethanol; aliphatic hydrocarbon such as hexane, heptane, octane; ether such as diethyl ether, diisopropyl ether, tetrahydrofuran, dioxane; aromatic hydrocarbon such as benzene, toluene, xylene, mesitylene; halogenated hydrocarbon such as dichloromethane, chloroform, carbon tetrachloride, 1,2-dichloroethane are exemplified. Of these, aliphatic hydrocarbon such as hexane, heptane, octane; alcohol such as methanol, ethanol are more preferable. The amount of the solvent to be used is free of any particular limitation but it is generally preferably 1 to 100-fold weight, more preferably 1 to 10-fold weight, relative to azabicyclohept-3-ene (VI). The reaction temperature is preferably -50 to 100°C, more preferably -20 to 50°C. The 7-azabicyclo[4.1.0]hept-3-ene-3-carboxylic acid ester (VII) thus obtained can be purified and separated according to a method generally employed for the purification and separation of organic compounds. For example, the reaction mixture is neutralized or filtrated, then concentrated, and the obtained concentrate is purified by distillation, column chromatography. (e): step to protect amino group of aminodiol (III) to give aminodiol (VIII) As the protecting group used to protect amino group of aminodiol (III), the protecting groups generally used for the protection of amino group can be used. Of these protecting groups, those stable under the reaction conditions, under which to produce disulfonate (IX) in the next step (f), and capable of being removed when the protecting group is to be quickly removed in step (g) to be mentioned below, without impairing other moieties of disulfonate (X) are particularly preferable. Examples of the amino-protecting group include alkoxycarbonyl group such as tert-butoxycarbonyl group, 2,2,2-trichloroethoxycarbonyl group, 1,1-dimethylpropyloxycarbonyl group; aralkyloxycarbonyl group such as p-methoxybenzyloxycarbonyl group. Of these, tert-butoxycarbonyl group is particularly preferable. These amino-protecting groups can be introduced by a known method (T. W. Green, "Protective Groups in Organic Synthesis," John-Wiley & Sons, New York, pp 218-287 (1981)). For example, tert-butoxycarbonyl group can be introduced by the use of di-tert-butyl dicarbonate in an amount of 1 to 3-fold moles, more preferably 1 to 1.2-fold moles, relative to aminodiol (III). The reaction to protect amino group is preferably carried out in the presence of a solvent. The solvent to be used is free of any particular limitation as long as it does not adversely affect the reaction and examples thereof include halogenated hydrocarbon such as dichloromethane, chloroform, carbon tetrachloride, 1,2-dichloroethane; aliphatic hydrocarbon such as hexane, heptane, octane ; aromatic hydrocarbon such as benzene, toluene, xylene, mesitylene; ether such as diethyl ether, diisopropyl ether, tetrahydrofuran, dioxane; acetonitrile. The amount of the solvent to be used is free of any particular limitation, but generally it is preferably 1 to 100-fold weight, more preferably 1 to 10-fold weight, relative to aminodiol (III). The reaction temperature is preferably -20 to 150°C, more preferably 20 to 80°C. The aminodiol (VIII) thus obtained can be purified and separated according to a method generally employed for the purification and separation of organic compounds. For example, the reaction mixture is poured into water, extracted with aliphatic hydrocarbon such as n-hexane, aromatic hydrocarbon such as toluene, ether such as diethyl ether, diisopropyl ether and the extract is concentrated and the obtained concentrate is purified by distillation, column chromatography. (f): step for reacting aminodiol (VIII) with a sulfonylating agent in the presence of a base to give disulfonate (IX) As the sulfonylating agent, the compounds recited in the above-mentioned step (b) can be used. The amount of the sulfonylating agent to be used is preferably 2 to 10-fold moles more preferably 2 to 5-fold moles, per 1 mole of aminodiol (VIII). Examples of the base include tertiary amine such as trimethylamine, triethylamine, tripropylamine, tributylamine, trioctylamine, pyridine, collidine, lutidine; alkaline metal hydride such as sodium hydride, potassium hydride; alkaline metal carbonate such as sodium carbonate, potassium carbonate. Of these, triethylamine, pyridine and lutidine are preferable. The amount of the base to be used is preferably 2 to 10-fold moles, more preferably 2 to 5-fold moles, per 1 mole of aminodiol (VIII). The reaction is preferably carried out in the presence of a. solvent. The solvent to be used is free of any particular limitation as long as it is not involved in the reaction. Examples thereof include aliphatic hydrocarbon such as hexane, heptane, octane; aromatic hydrocarbon such as benzene, toluene, xylene, mesitylene; ether such as diethyl ether, diisopropyl ether, tetrahydrofuran, dioxane; halogenated hydrocarbon such as dichloromethane, chloroform, carbon tetrachloride, 1,2-dichloroethane. The amount of the solvent to be used is free of any particular limitation but it is generally preferably 1 to 100-fold weight, more preferably 1 to 10-fold weight, relative to aminodiol (VIII). The reaction temperature is preferably 0-50°C, more preferably 10-30°C. While the reaction time varies depending on the kind and the amount of aminodiol (VIII), base, sulfonylating agent and solvent, it is generally 1 to 48 hours. The reaction is preferably carried out by, for example, dissolving aminodiol (VIII) and a base in a solvent, adjusting to a given temperature, adding a sulfonylating agent and stirring the mixture. The disulfonate (IX) thus obtained can be purified and separated according to a method generally employed for the purification and separation of organic compounds. For example, the reaction mixture is poured into water, extracted with aliphatic hydrocarbon such as n-hexane, aromatic hydrocarbon such as toluene, ether such as diethyl ether, diisopropyl ether, and the extract is concentrated and the obtained concentrate is purified by distillation, column chromatography. (g): step to remove amino-protecting group from disulfonate (IX) to give disulfonate (X) The amino-protecting group can be removed from disulfonate (IX) according to a known method (T. W. Green, "Protective Groups in Organic Synthesis," John-Wiley & Sons, New York, pp 218-287 (1981)). When the amino-protecting group is tert-butoxycarbonyl group, it can be removed by using trifluoroacetic acid, trimethylbromosilane, trimethyliodosilane and halogenated hydrocarbon such as dichloromethane, chloroform as a solvent in a proportion of 1 to 100-fold weight, preferably 1 to 10-fold weight, relative to disulfonate (IX), at a reaction temperature of 0-50°C, preferably 10-30°C. The obtained disulfonate (X) can be purified and separated according to a method generally employed for the purification and separation of organic compounds. For example, the reaction mixture is poured into water, extracted with aliphatic hydrocarbon such as n-hexane, aromatic hydrocarbon such as toluene, ether such as diethyl ether, diisopropyl ether, and the extract is concentrated and the obtained concentrate is purified by distillation, column chromatography. (h): step to react disulfonate (X) with a base to obtain azabicyclohept-2-ene (IV) Examples of the base include tertiary amine such as trimethylamine, triethylamine, tripropylamine, tributylamine, trioctylamine, pyridine, collidine, lutidine; alkaline metal hydride such as sodium hydride, potassium hydride; alkaline metal carbonate such as sodium carbonate, potassium carbonate. Of these, triethylamine, pyridine and lutidine are preferable. The amount of the base to be used is preferably 2 to 100-fold moles, more preferably 2 to 25-fold moles, per 1 mole of disulfonate (X). The reaction is preferably carried out in the presence of a solvent. The solvent to be used is free of any particular limitation as long as it is not involved in the reaction. Examples thereof include aliphatic hydrocarbon such as hexane, heptane, octane; aromatic hydrocarbon such as benzene, toluene, xylene, mesitylene; ether such as diethyl ether, diisopropyl ether, tetrahydrofuran, dioxane; halogenated hydrocarbon such as dichloromethane, chloroform, carbon tetrachloride, 1,2-dichloroethane. The amount of the solvent to be used is free of any particular limitation, and generally it is preferably 1 to 100-fold weight, more preferably 1 to 10-fold weight, relative to disulfonate (X). The reaction temperature is preferably 0-150°C, more preferably 20-80°C. While the reaction time varies depending on the kind and the amount of use of disulfonate (X), base and solvent, it is generally 1 to 48 hours. The reaction is preferably carried out by, for example, dissolving disulfonate (X) and a base in a solvent and stirring the mixture at a given temperature. The azabicyclohept-2-ene (IV) thus obtained can be purified and separated according to a method generally employed for the purification and separation of organic compounds. For example, the reaction mixture is poured into water, extracted with aliphatic hydrocarbon such as n-hexane, aromatic hydrocarbon such as toluene, ether such as diethyl ether, diisopropyl ether, and the extract is concentrated and the obtained concentrate is purified by distillation, column chromatography. The epoxide (I), which is a starting material for the present invention, can be produced as shown in Reference Examples 1-3 to be mentioned below, wherein furan and acrylic acid ester of the formula (XI) wherein R 1 is as defined above, are subjected to a Diels-Alder reaction in the presence of Lewis acid, such as zinc iodide, zinc chloride, titanium tetrachloride, the obtained compound is reacted with a base such as lithium diisopropylamide, lithium hexamethyldisilazide to perform intramolecular retro-Michael reaction to give dieno alcohol of the formula (XII) wherein R 1 is as defined above [see Tetrahedron Letters, vol. 23, p. 5299 (1982)], and this dieno alcohol is reacted with peroxide such as magnesium monoperoxyphthalate, peracetic acid, m-chloroperbenzoic acid [see Journal of American Chemical Society (J. Am. Chem. Soc.), vol. 104, p. 7036 (1982)]. According to the method described in Journal of Organic Chemistry (J. Org. Chem.), vol. 63, p. 4545 (1998), GS4104 can be synthesized from 7-azabicyclo[4.1.0]hept-3-ene-3-carboxylic acid ester (VII), such as ethyl (1α,5α,6α)-5-(1-ethylpropoxy)-7-azabicyclo[4.1.0]hept-3-ene-3-carboxylate obtained by the method of the present invention. Examples The present invention is described in more detail by means of the following Examples, which are not to be construed as limitative. Reference Example 1: Synthesis of 2-ethoxycarbonyl-7-oxabicyclo[2.2.1]hept-5-ene Furan (2.04 ml, 28 mmol), ethyl acrylate (2.06 ml, 20 mmol) and zinc iodide (1.92 g, 6 mmol) were placed in a sealed tube and the mixture was heated at 40°C for 2 days. The reaction mixture was cooled to room temperature and diluted with ethyl acetate (100 ml). 10% Aqueous sodium thiosulfate solution (20 ml) was added, and the mixture was stirred at room temperature for 30 min. The organic layer was partitioned, washed with saturated brine (100 ml), and dried over anhydrous sodium sulfate. The components having a low boiling point, such as the solvent, were distilled away. The obtained residue was purified by silica gel column chromatography (eluent: ethyl acetate/hexane = 1/10 (volume ratio)) to give 2-ethoxycarbonyl-7-oxabicyclo[2.2.1]hept-5-ene as a mixture of exo form:endo form = 7:3 (1.75 g, 10.4 mmol, yield 52.0%). IR(neat, cm -1 ): 2986, 1734, 1450, 1369, 1340, 1315, 1277, 1192, 1095, 1047, 1024 1 H-NMR (250MHz, CDCl 3 , TMS, ppm) δ: exo form: 6.42-6.32 (2H, m), 5.19 (1H, s), 5.08 (1H, d, J=4.5Hz), 4.19 (2H, q, J=7.2Hz), 2.45-2.40 (1H, m), 2.22-2.19 (1H, m), 1.68-1.48 (1H, m), 1.28 (3H, t, J=7.2Hz) endo form: 6.46-6.43 (1H, m), 6.25-6.22 (1H, m), 5.18-5.16 (1H, m), 5.03-5.01 (1H, m), 4.10 (2H, q, J=7.2Hz), 3.18-3.05 (1H, m), 2.18-2.02 (1H, m), 1.68-1.52 (1H, m), 1.24 (3H, t, J=7.2Hz) EIMS m/z: 139[(M-C 2 H 5 ) + ], 123[(M-C 2 H 5 O) + ], 99, 68, 55, 39, 28 HRMS calcd for C 9 H 12 O 3 (M + ): 168.0786, Found m/z = 168.0771 Reference Example 2: Synthesis of ethyl 5-hydroxy-1,3-cyclohexadiene-1-carboxylate To a solution (10.72 ml, 1.0 mol/l, 10.72 mmol) of lithium hexamethyldisilazide in tetrahydrofuran was added tetrahydrofuran (60 ml), and the mixture was cooled to -78°C. To this solution was added dropwise a solution of 2-ethoxycarbonyl-7-oxabicyclo[2.2.1]hept-5-ene (a mixture of exo form:endo form = 7:3, 1.64 g, 9.75 mmol, obtained in Reference Example 1) in tetrahydrofuran (12 ml). After the dropwise addition, the mixture was heated to 0°C and stirred for 1.5 h. To this reaction mixture was added saturated aqueous ammonium chloride solution (50 ml), and the mixture was extracted with chloroform (100 ml). The organic layer was partitioned, washed with saturated brine (20 ml), and dried over anhydrous sodium sulfate. The components having a low boiling point, such as the solvent, were distilled away. The obtained residue was purified by silica gel column chromatography (eluent: ethyl acetate/hexane = 1/5 (volume ratio)) to give ethyl 5-hydroxy-1,3-cyclohexadiene-1-carboxylate (1.52 g, 9.06 mmol, yield 92.9%). IR(neat, cm -1 ): 3412, 3045, 2982, 2937, 2906, 1705, 1641, 1577, 1446, 1402, 1367, 1255, 1203, 1099, 1059, 1026 1 H-NMR (250MHz, CDCl 3 , TMS, ppm) δ: 7.12-7.03 (1H, m), 6.31-6.18 (2H, m), 4.45-4.32 (1H, m), 4.23 (2H, q, J=7.2Hz), 3.00-2.85 (1H, m), 2.71-2.54 (1H, m), 1.58 (1H, s), 1.31 (3H, t, J=7.2Hz) Reference Example 3: Synthesis of ethyl (1β,5α,6β)-5-hydroxy-7-oxabicyclo[4.1.0]hept-2-ene-3-carboxylate Ethyl 5-hydroxy-1,3-cyclohexadiene-1-carboxylate (2.84 g, 16.88 mmol), obtained according to the method of Reference Example 2, was dissolved in dichloromethane (20 ml), and sodium hydrogen carbonate (7.09 g, 84.4 mmol) was added. The mixture was cooled to 0°C and to this solution was added dropwise a solution of 80% m-chloroperbenzoic acid (4.00 g, 18.57 mmol) in dichloromethane (30 ml). After the dropwise addition, the mixture was heated to room temperature and stirred for 12 h. The solid in the reaction mixture was filtered off, and the filtrate was washed with 5% aqueous sodium sulfite solution (30 ml) and saturated brine (20 ml), and dried over anhydrous sodium sulfate. The components having a low boiling point, such as the solvent, were distilled away. The obtained residue was purified by silica gel column chromatography (eluent: ethyl acetate/hexane = 1/5 (volume ratio)) to give ethyl (1β,5α,6β)-5-hydroxy-7-oxabicyclo[4.1.0]hept-2-ene-3-carboxylate (2.61 g, 14.17 mmol, yield 83.9%). IR(neat, cm -1 ): 3435, 2984, 2908, 1712, 1645, 1373, 1251, 1188, 1091, 1057 1 H-NMR (250MHz, CDCl 3 , TMS, ppm) δ: 7.03-7.00 (1H, m), 4.21 (2H, q, J=7.2Hz), 4.22-4.06 (1H, m), 3.69-3.61 (1H, m), 3.56-3.45 (1H, m), 3.01-2.83 (1H, m), 2.00 (1H, s), 1.29 (3H, t, J=7.2Hz) EIMS m/z: 166[(M-2H 2 O) + ], 155[(M-C 2 H 5 ) + ], 138[(M-C 2 H 5 -H 2 O) + ], 110[(M-CO 2 C 2 H 5 ) + ], 82, 53 HRMS: calcd for C 9 H 12 O 4 (M + ) 184.0735, Found m/z = 184.0726 Example 1: Synthesis of ethyl (3β,4α,5α)-3-benzhydrylamino-4,5-dihydroxy-1-cyclohexene-1-carboxylate Ethyl (1β,5α,6β)-5-hydroxy-7-oxabicyclo[4.1.0]hept-2-ene-3-carboxylate (245 mg, 1.33 mmol) obtained in Reference Example 3 was dissolved in tetrahydrofuran (0.5 ml), and benzhydrylamine (365 mg, 2.00 mmol) was added. The mixture was stirred at 65°C for 2 days. The reaction mixture was cooled to room temperature, and the components having a low boiling point, such as the solvent, were distilled away. The obtained residue was purified by silica gel column chromatography (eluent: ethyl acetate/hexane = 1/1 (volume ratio)) to give ethyl (3β,4α,5α)-3-benzhydrylamino-4,5-dihydroxy-1-cyclohexene-1-carboxylate (457 mg, 1.24 mmol, yield 93.5%). IR(neat, cm -1 ): 3437, 3061, 3028, 2984, 2906, 1709, 1649, 1452, 1367, 1265, 1076, 1047 1 H-NMR (250MHz, CDCl 3 , TMS, ppm) δ: 7.46-7.22 (10H, m), 6.99-6.98 (1H, m), 5.16 (1H, s), 4.20 (2H, q, J=7.2Hz), 3.53-3.38 (2H, m), 2.55-2.54 (2H, m), 1.29 (3H, t, J=7.2Hz) EIMS m/z: 367(M + ), 307[(M-C 2 H 4 O 2 ) + ], 200[(M-CHPh 2 ) + ], 167 HRMS: calcd for C 22 H 25 NO 4 (M + ) 367.1784, Found m/z = 367.1785 Example 2: Synthesis of ethyl (1α,5α,6α)-7-diphenylmethyl-5-methanesulfonyloxy-7-azabicyclo[4.1.0]hept-2-ene-3-carboxylate Ethyl (3β,4α,5α)-3-benzhydrylamino-4,5-dihydroxy-1-cyclohexene-1-carboxylate (110 mg, 0.299 mmol) obtained in Example 1 was dissolved in tetrahydrofuran (2 ml), and triethylamine (1 ml) was added. The mixture was stirred at room temperature for 5 min and cooled to 0°C. To this solution was added dropwise methanesulfonyl chloride (103 mg, 0.687 mmol), and after the dropwise addition, the mixture was heated to 65°C and stirred for 24 h. The reaction mixture was cooled to room temperature and concentrated. The residue was diluted with dichloromethane (20 ml), washed with saturated aqueous sodium hydrogen carbonate solution (20 ml) and saturated brine (10 ml), and dried over anhydrous sodium sulfate. The components having a low boiling point, such as the solvent , were distilled away. The obtained residue was purified by silica gel column chromatography (eluent: ethyl acetate/hexane = 1/3 (volume ratio)) to give ethyl (1α,5α,6α)-7-diphenylmethyl-5-methanesulfonyloxy-7-azabicyclo[4.1.0]hept-2-ene-3-carboxylate (71.3 mg, 0.167 mmol, yield 55.7%). IR(neat, cm -1 ): 3022, 1705, 1359, 1267, 1217, 1172, 1095 1 H-NMR (250MHz, CDCl 3 , TMS, ppm) δ: 7.23-7.19 (11H, m), 5.32-5.28 (1H, m), 4.21 (2H, q, J=7.2Hz), 3.83 (1H, s), 3.09-2.98 (1H, m), 2.97 (3H, s), 2.68-2.55 (2H, m), 2.41-2.32 (1H, m), 1.31 (3H, t, J=7.2Hz) EIMS m/z: 331[(M-O 3 SCH 3 ) + ], 167 HRMS calcd for C 23 H 25 NO 5 S(M + ): 427.1454, Found m/z = 427.1452 Example 3: Synthesis of ethyl (1α,5α,6α)-7-diphenylmethyl-5-(1-ethylpropoxy)-7-azabicyclo[4.1.0]hept-3-ene-3-carboxylate Ethyl (1α,5α,6α)-7-diphenylmethyl-5-methanesulfonyloxy-7-azabicyclo[4.1.0]hept-2-ene-3-carboxylate (235 mg, 0.55 mmol) qbtained according to the method of Example 2, 3-pentanol (6 ml) and dichloromethane (2 ml) were mixed and dissolved. To this solution was added boron trifluoride etherate (390 mg, 2.45 mmol) at room temperature, and the mixture was stirred at room temperature for 3 h. To this reaction mixture was added triethylamine (556 mg, 5.49 mmol) at room temperature, and the mixture was stirred at room temperature for 1 more h. The reaction mixture was concentrated, and the residue was diluted with diethyl ether (20 ml), washed with saturated aqueous sodium hydrogen carbonate solution (10 ml) and saturated brine (5 ml), and dried over anhydrous sodium sulfate. The components having a low boiling point, such as the solvent, were distilled away. The obtained residue was purified by silica gel column chromatography (eluent: ethyl acetate/hexane = 1/3 (volume ratio)) to give ethyl (1α,5α,6α)-7-diphenylmethyl-5-(1-ethylpropoxy)-7-azabicyclo[4.1.0]hept-3-ene-3-carboxylate (197 mg, 0.47 mmol, yield 85.6%). IR(neat, cm -1 ): 2968, 2934, 2876, 1714, 1660, 1493, 1302, 1248, 1080, 1060 1 H-NMR (250MHz, CDCl 3 , TMS, ppm) δ: 7.41-7.21 (10H, m), 6.81-6.75 (1H, m), 4.19 (2H, q, J=7.2Hz), 4.10-4.01 (1H, m), 3.71 (1H, s), 3.17-3.07 (1H, m), 2.76-2.65 (1H, m), 2.64-2.48 (1H, m), 2.18-2.08 (1H, m), 2.00-1.91 (1H, m), 1.49-1.34 (4H, m), 1.31 (3H, t, J=7.2Hz), 0.86-0.78 (6H, m) EIMS m/z: 419(M + ), 346[(M-CO 2 C 2 H 5 ) + ], 305, 167 HRMS: calcd for C 27 H 33 NO 3 (M + ): 419.2461, Found m/z = 419.2464 Example 4: Synthesis of ethyl (1α,5α,6α)-5-(1-ethylpropoxy)-7-azabicyclo[4.1.0]hept-3-ene-3-carboxylate Ethyl (1α,5α,6α)-7-diphenylmethyl-5-(1-ethylpropoxy)-7-azabicyclo[4.1.0]hept-3-ene-3-carboxylate (17 mg, 0.0405 mmol) obtained in Example 3 and 10% palladium-carbon (17 mg) were placed in a flask and the mixture was cooled to 0°C. Thereto was added formic acid (4.4% methanol solution, 1 ml) cooled to 0°C and the mixture was stirred at 0°C for 3 h. The reaction mixture was filtrated and the filtrate was concentrated. The residue was diluted with dichloromethane (10 ml), washed with saturated aqueous sodium hydrogen carbonate solution (10 ml) and saturated brine (5 ml), and dried over anhydrous sodium sulfate. The components having a low boiling point, such as the solvent, were distilled away. The obtained residue was purified by silica gel column chromatography (eluent: ethyl acetate/hexane = 1/4 → 5/1 (volume ratio)) to give ethyl (1α,5α,6α)-5-(1-ethylpropoxy)-7-azabicyclo[4.1.0]hept-3-ene-3-carboxylate (4.3 mg, 0.0169 mmol, yield 41.7%), and the starting material: ethyl (1α,5α,6α)-7-diphenylmethyl-5-(1-ethylpropoxy)-7-azabicyclo[4.1.0]hept-3-ene-3-carboxylate (6.75 mg) was recovered (0.0161 mmol, recovery yield 39.7%). IR(neat, cm -1 ): 2962, 2926, 2854, 1716, 1462, 1381, 1251, 1130, 1084, 1057 1 H-NMR (500MHz, CDCl 3 , TMS, ppm) δ: 6.79-6.78 (1H, m), 4.33-4.32 (1H, m), 4.18 (2H, q, J=7.2Hz), 3.41-3.38 (1H, m), 2.84-2.80 (1H, m), 2.64-2.60 (1H, m), 2.53-2.51 (1H, m), 2.43-2.42 (1H, m), 1.58-1.53 (1H, m), 1.27 (3H, t, J=7.2Hz), 0.96 (3H, t, J=7.5Hz), 0.91 (3H, t, J=7.5Hz) EIMS m/z: 253(M + ), 224[(M-C 2 H 5 ) + ], 208[(M-OC 2 H 5 ) + ], 182[(M-C 5 H 11 ) + ], 166[(M-OC 5 H 11 ) + ], 154, 137, 110, 93 HRMS calcd for C 14 H 23 NO 3 (M + ): 253.1678, Found m/z = 253.1695 Example 5: Synthesis of ethyl (3β,4α,5α)-3-benzylamino-4,5-dihydroxy-1-cyclohexene-1-carboxylate Ethyl (1β,5α,6β)-5-hydroxy-7-oxabicyclo[4.1.0]hept-2-ene-3-carboxylate (1.00 g, 5.42 mmol) obtained in Reference Example 3 was dissolved in tetrahydrofuran (1 ml), and benzylamine (0.652 g, 5.96 mmol) was added. The mixture was stirred at 50°C for 18 h and the reaction mixture was cooled to room temperature. The components having a low boiling point, such as the solvent were distilled away. The obtained residue was purified by silica gel column chromatography (eluent: ethyl acetate/hexane = 5/1 (volume ratio)) to give ethyl (3β,4α,5α)-3-benzylamino-4,5-dihydroxy-1-cyclohexene-1-carboxylate (1.49 g, 5.10 mmol, yield 94.1%). IR(neat, cm -1 ): 3402, 2982, 2905, 1709, 1651, 1454, 1367, 1255, 1080 1 H-NMR (250MHz, CDCl 3 , TMS, ppm) δ: 7.39-7.26 (5H, m), 6.96 (1H, s), 4.29-4.21 (1H, m), 4.21 (2H, q, J=7.2Hz), 3.93 (2H, dd, J=53, 13Hz), 3.48 (1H, s), 3.52-3.40 (1H, m), 2.82-2.45 (2H, m), 1.29 (3H, t, J=7.2Hz) EIMS m/z: 291(M + ), 255[(M-2H 2 O) + ], 231, 91 HRMS calcd for C 16 H 21 NO 4 (M + ): 291.1471, Found m/z = 291.1457 Example 6: Synthesis of ethyl (3β,4α,5α)-3-{N-(tert-butoxycarbonyl)-N-benzylamino}-4,5-dihydroxy-1-cyclohexene-1-carboxylate Ethyl (3β,4α,5α)-3-benzylamino-4,5-dihydroxy-1-cyclohexene-1-carboxylate (1.48 g, 5.07 mmol) obtained in Example 5 was dissolved in acetonitrile (10 ml), and di-tert-butyl dicarbonate (1.22 g, 5.58 mmol) was added. The mixture was stirred at room temperature for 1.5 h and, after the completion of the reaction, the components having a low boiling point, such as the solvent, were distilled away. The obtained residue was purified by silica gel column chromatography (eluent: ethyl acetate/hexane = 1/3 (volume ratio)) to give ethyl (3β,4α,5α)-3-{N-(tert-butoxycarbonyl)-N-benzylamino}-4,5-dihydroxy-1-cyclohexene-1-carboxylate (1.75 g, 4.47 mmol, yield 88.2%). IR(neat, cm -1 ): 3441, 3011, 2980, 2934, 1693, 1454, 1410, 1367, 1253, 1165, 1084 EIMS m/z: 391(M + ), 335[(M-C 4 H 8 ) + ], 290[(M-C 4 H 9 CO 2 ) + ], 231, 91 HRMS calcd for C 21 H 29 NO 6 (M + ): 391.1995, Found m/z = 391.1987 Example 7: Synthesis of ethyl (3β,4α,5α)-3-{N-(tert-butoxycarbonyl)-N-benzylamino}-4,5-dimethanesulfonyloxy-1-cyclohexene-1-carboxylate Ethyl (3β,4α,5α)-3-{N-(tert-butoxycarbonyl)-N-benzylamino}-4,5-dihydroxy-1-cyclohexene-1-carboxylate (1.73 g, 4.42 mmol) obtained in Example 6 was dissolved in methylene chloride (20 ml), and triethylamine (1.12 g, 11.05 mmol) was added. The mixture was stirred at room temperature for 5 min and this reaction mixture was cooled to 0°C. Methanesulfonyl chloride (1.11 g, 9.72 mmol) was added dropwise over 5 min and, after the dropwise addition, the mixture was stirred at room temperature for 1.5 h. The components having a low boiling point, such as the solvent, were distilled away. The obtained residue was purified by silica gel column chromatography (eluent: ethyl acetate/hexane = 1/5 (volume ratio)) to give ethyl (3β,4α,5α)-3-{N-(tert-butoxycarbonyl)-N-benzylamino}-4,5-dimethanesulfonyloxy-1-cyclohexene-1-carboxylate (2.41 g, 4.40 mmol, yield 99.6%). IR(neat, cm -1 ): 2980, 2939, 1701, 1454, 1419, 1359, 1246, 1176, 1091, 1057, 1005 EIMS m/z: 491[(M-C 4 H 8 ) + ], 381, 244, 150, 106, 91 HRMS calcd for C 19 H 25 NO 10 S 2 [(M-C 4 H 8 ) + ]: 491.0920, Found m/z = 491.0901 Example 8: Synthesis of ethyl (3β,4α,5α)-3-benzylamino-4,5-dimethanesulfonyloxy-1-cyclohexene-1-carboxylate Ethyl (3β,4α,5α)-3-{N-(tert-butoxycarbonyl)-N-benzylamino}-4,5-dimethanesulfonyloxy-1-cyclohexene-1-carboxylate (2.11 g, 3.85 mmol) obtained according to the method of Example 7 was dissolved in methylene chloride (66 ml), and trifluoroacetic acid (4.4 g, 38.5 mmol) was added. The mixture was stirred at room temperature for 18 h. The reaction mixture was washed with saturated aqueous sodium hydrogen carbonate solution (50 ml) and saturated brine (20 ml), and dried over anhydrous sodium sulfate. The components having a low boiling point, such as the solvent were distilled away. The obtained residue was purified by silica gel column chromatography (eluent: ethyl acetate/hexane = 1/5 (volume ratio)) to give ethyl (3β,4α,5α)-3-benzylamino-4,5-dimethanesulfonyloxy-1-cyclohexene-1-carboxylate (1.65 g, 3.69 mmol, yield 96.0%). IR(neat, cm -1 ): 3028, 2984, 2939, 2361, 1712, 1653, 1454, 1359, 1249, 1176, 1095, 1045, 1003 1 H-NMR (250MHz, CDCl 3 , TMS, ppm) δ: 7.38-7.25 (5H, m), 6.87-6.79 (1H, m), 5.28-5.18 (1H, m), 4.89-4.79 (1H, m), 4.22 (2H, q, J=7.2Hz), 3.92 (2H, dd, J=22.5, 14.2Hz), 3.81-3.70 (1H, m), 3.12 (1H, s), 3.19 (1H, s), 2.88-2.78 (2H, m), 1.30 (3H, t, J=7.2Hz) Example 9: Synthesis of ethyl (1α,5α,6α)-7-benzyl-5-methanesulfonyloxy-7-azabicyclo[4.1.0]hept-2-ene-3-carboxylate Ethyl (3β,4α,5α)-3-benzylamino-4,5-dimethanesulfonyloxy-1-cyclohexene-1-carboxylate (0.278 g, 0.62 mmol) obtained in Example 8 was dissolved in tetrahydrofuran (1 ml), and triethylamine (1 ml) was added. The mixture was stirred at 50°C for 2 days. The reaction mixture was cooled to room temperature. The components having a low boiling point, such as the solvent were distilled away. The obtained residue was purified by silica gel column chromatography (eluent: ethyl acetate/hexane = 1/3 (volume ratio)) to give ethyl (1α,5α,6α)-7-benzyl-5-methanesulfonyloxy-7-azabicyclo[4.1.0]hept-2-ene-3-carboxylate (0.124 g, 0.352 mmol, yield 56.7%). IR(neat, cm -1 ): 1707, 1358, 1265, 1209, 1172, 1095 1 H-NMR (250MHz, CDCl 3 , TMS, ppm) δ: 7.39-7.20 (6H, m), 5.47-5.37 (1H, m), 4.19 (2H, q, J=7.2Hz), 3.59 (2H, dd, J=75, 14Hz), 3.00 (1H, s), 3.05-2.89 (1H, m), 2.65-2.38 (2H, m), 2.34-2.22 (1H, m), 1.28 (3H, t, J=7.2Hz) EIMS m/z: 351(M + ), 256[(M-O 3 SCH 3 ) + ], 164, 91 HRMS calcd for C 17 H 21 NO 5 S(M + ): 351.1141, Found m/z = 351.1110 Example 10: Synthesis of ethyl (1α,5α,6α)-7-benzyl-5-(1-ethylpropoxy)-7-azabicyclo[4.1.0]hept-3-ene-3-carboxylate Ethyl (1α,5α,6α)-7-benzyl-5-methanesulfonyloxy-7-azabicyclo[4.1.0]hept-2-ene-3-carboxylate (50 mg, 0.142 mmol) obtained in Example 9 was dissolved in 3-pentanol (1 ml), and boron trifluoride etherate (22.2 mg, 0.156 mmol) was added at room temperature. The mixture was stirred at 65°C for 3 h. The reaction mixture was cooled to room temperature. Triethylamine (21.6 mg, 0.213 mmol) was added and the mixture was stirred at room temperature for 1 h. The reaction mixture was concentrated, and the residue was diluted with diethyl ether (10 ml), washed with saturated aqueous sodium hydrogen carbonate solution (5 ml) and saturated brine (3 ml), and dried over anhydrous sodium sulfate. The components having a low boiling point, such as the solvent, were distilled away. The obtained residue was purified by silica gel column chromatography (eluent: ethyl acetate/hexane = 1/3 (volume ratio)) to give ethyl (1α,5α,6α)-7-benzyl-5-(1-ethylpropoxy)-7-azabicyclo[4.1.0]hept-3-ene-3-carboxylate (42.8 mg, 0.125 mmol, yield 87.7%). IR(neat, cm -1 ): 2968, 2934, 2876, 1714, 1454, 1369, 1302, 1248, 1080, 1059 1 H-NMR (250MHz, CDCl 3 , TMS, ppm) δ: 7.33-7.28 (5H, m), 6.78-6.77 (1H, m), 4.30-4.20 (1H, m), 4.16 (2H, q, J=7.2Hz), 3.58 (1H, s), 3.40-3.21 (1H ,m), 2.83-2.66 (1H, m), 2.65-2.48 (1H, m), 2.11-2.00 (1H, m), 2.00-1.88 (1H, m), 1.68-1.43 (4H, m), 1.25 (3H, t, J=7.2Hz), 0.93 (3H, t, J=7.4Hz), 0.89 (3H, t, J=7.4Hz) EIMS m/z: 343(M + ), 314[(M-C 2 H 5 ) + ], 272[(M-C 5 H 11 ) + ], 256, 200, 91 HRMS calcd for C 21 H 29 NO 3 (M + ): 343.2148, Found m/z = 343.2147 Example 11: Synthesis of ethyl (1α,5α,6α)-5-(1-ethylpropoxy)-7-azabicyclo[4.1.0]hept-3-ene-3-carboxylate Ethyl (1α,5α,6α)-7-benzyl-5-(1-ethylpropoxy)-7-azabicyclo[4.1.0]hept-3-ene-3-carboxylate (19 mg, 0.055 mmol) obtained in Example 10 and 10% palladium-carbon (19 mg) were placed in a flask and formic acid (4.4% methanol solution, 1 ml) was added at room temperature. The mixture was stirred at room temperature for 10 min. The reaction mixture was filtrated and the filtrate was concentrated. The residue was diluted with dichloromethane (10 ml), washed with saturated aqueous sodium hydrogen carbonate solution (10 ml) and saturated brine (5 ml), and dried over anhydrous sodium sulfate. The components having a low boiling point, such as the solvent, were distilled away. The obtained residue was purified by silica gel column chromatography (eluent: ethyl acetate/hexane = 1/4 → 5/1 (volume ratio)) to give ethyl (1α,5α,6α)-5-(1-ethylpropoxy)-7-azabicyclo[4.1.0]hept-3-ene-3-carboxylate (8.1 mg, 0.0319 mmol, yield 58.0%). IR(neat, cm -1 ): 2962, 2926, 2854, 1716, 1462, 1381, 1251, 1130, 1084, 1057 1 H-NMR (500MHz, CDCl 3 , TMS, ppm) δ: 6.79-6.78 (1H, m), 4.33-4.32 (1H, m), 4.18 (2H, q, J=7.2Hz), 3.41-3.38 (1H, m), 2.84-2.80 (1H, m), 2.64-2.60 (1H, m), 2.53-2.51 (1H, m), 2.43-2.42 (1H, m), 1.58-1.53 (1H, m), 1.27 (3H, t, J=7.2Hz), 0.96 (3H, t, J=7.5Hz), 0.91 (3H, t, J=7.5Hz) EIMS m/z: 253(M + ), 224[(M-C 2 H 5 ) + ], 208[(M-OC 2 H 5 ) + ], 182[(M-C 5 H 11 ) + ], 166[(M-OC 5 H 11 ) + ], 154, 137, 110, 93 HRMS calcd for C 14 H 23 NO 3 (M + ): 253.1678, Found m/z = 253.1695 The α and β show the relative configuration of the substituents on the ring. The relative configuration of Example compounds are shown in the following, where α is conveniently placed on the upper side of the sheet and β on the lower side of the sheet, but these structural formulas do not represent the absolute configuration. Dpm means diphenylmethyl, Bzl means benzyl, Boc means tert-butoxycarbonyl, Ms means methanesulfonyl and Et means ethyl. Industrial Applicability According to the present invention, 7-azabicyclo[4.1.0]hept-3-ene-3-carboxylic acid ester such as ethyl (1α,5α,6α)-5-(1-ethylpropoxy)-7-azabicyclo[4.1.0]hept-3-ene-3-carboxylate useful as a synthetic intermediate for GS4104 being developed as an anti-influenza drug can be produced economically, industrially advantageously and efficiently in a large amount. The epoxide (I), which is a starting material in the method of the present invention, can be synthesized from an economical starting material. Therefore, according to the method of the present invention, 7-azabicyclo[4.1.0]hept-3-ene-3-carboxylic acid ester can be produced from an economical starting material, without using an expensive starting material. The method of the present invention is suitable for producing 7-azabicyclo[4.1.0]hept-3-ene-3-carboxylic acid ester industrially in a large amount. This application is based on a patent application No. 11-312616 filed in Japan.

Claims: A production method of a 7-azabicyclo[4.1.0]hept-3-ene-3-carboxylic acid ester of the formula (VII) wherein R 1 is C 1-10 alkyl group which may have any substituent selected from C 1-10 alkoxyl group, halogen atom, cyano group and nitro group; or C 3-8 cycloalkyl group, C 6-10 aryl group or aralkyl group wherein the alkyl moiety is C 1-6 alkyl group and the aryl moiety has 1 to 3 C 6-10 aryl groups, where said cycloalkyl, aryl and aralkyl groups may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group and R 3 is C 1-10 alkyl group which may have any substituent selected from C 1-10 alkoxyl group, halogen atom, cyano group and nitro group; or C 3-8 cycloalkyl group, C 6-10 aryl group or aralkyl group wherein the alkyl moiety is C 1-6 alkyl group and the aryl moiety has 1 to 3 C 6-10 aryl groups, where said cycloalkyl, aryl and aralkyl groups may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group; or C 2-10 alkenyl group which may have any substituent selected from C 1-6 alkoxyl group, halogen atom, cyano group and nitro group, which comprises the steps of (A) reacting a 5-hydroxy-7-oxabicyclo[4.1.0]hept-2-ene-3-carboxylic acid ester of the formula (I) wherein R 1 is as defined above, with an amine of the formula (II) R 2 NH 2 wherein R 2 is a hydrogen atom, or C 1-10 alkyl group which may have any substituent selected from C 1-10 alkoxyl group, halogen atom, cyano group and nitro group; or C 3-8 cycloalkyl group, C 6-10 aryl group or aralkyl group wherein the alkyl moiety is C 1-6 alkyl group and the aryl moiety has 1 to 3 C 6-10 aryl groups, where said cycloalkyl, aryl and aralkyl groups may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group; or C 2-10 alkenyl group which may have any substituent selected from C 1-6 alkoxyl group, halogen atom, cyano group and nitro group, to give a 3-amino-4,5-dihydroxy-1-cyclohexene-1-carboxylic acid ester of the formula (III) wherein R 1 and R 2 are as defined above, (B) reacting the obtained 3-amino-4,5-dihydroxy-1-cyclohexene-1-carboxylic acid ester with a sulfonylating agent in the presence of a base to give a 5-sulfonyloxy-7-azabicyclo[4.1.0]hept-2-ene-3-carboxylic acid ester of the formula (IV) wherein R 1 and R 2 are as defined above and A is alkylsulfonyl group wherein the alkyl moiety is C 1-6 alkyl group, which may have any substituent selected from C 1-6 alkoxyl group, halogen atom, cyano group and nitro group; phenylsulfonyl group which may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group on the aromatic ring; or aralkylsulfonyl group wherein the aryl moiety is phenyl group and the alkyl moiety is C 1-6 alkyl group, which may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group on the aromatic ring, (C) reacting the obtained 5-sulfonyloxy-7-azabicyclo[4.1.0]hept-2-ene-3-carboxylic acid ester with an alcohol of the formula (V) R 3 OH wherein R 3 is as defined above, in the presence of a Lewis acid to give a 5-oxy-7-azabicyclo[4.1.0]hept-3-ene-3-carboxylic acid ester of the formula (VI) wherein R 1 , R 2 and R 3 are as defined above, and (D) when R 2 is a C 1-10 alkyl group which may have any substituent selected from C 1-10 alkoxyl group, halogen atom, cyano group and nitro group; or C 3-8 cycloalkyl group, C 6-10 aryl group or aralkyl group wherein the alkyl moiety is C 1-6 alkyl group and the aryl moiety has 1 to 3 C 6-10 aryl groups, where said cycloalkyl, aryl and aralkyl groups may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group; or C 2-10 alkenyl group which may have any substituent selected from C 1-6 alkoxyl group, halogen atom, cyano group and nitro group, eliminating the 7-position substituent R 2 of the obtained 5-oxy-7-azabicyclo[4.1.0]hept-3-ene-3-carboxylic acid ester (VI). A production method of a 7-azabicyclo[4.1.0]hept-3-ene-3-carboxylic acid ester of the formula (VII) wherein R 1 is C 1-10 alkyl group which may have any substituent selected from C 1-10 alkoxyl group, halogen atom, cyano group and nitro group; or C 3-8 cycloalkyl group, C 6-10 aryl group or aralkyl group wherein the alkyl moiety is C 1-6 alkyl group and the aryl moiety has 1 to 3 C 6-10 aryl groups, where said cycloalkyl, aryl and aralkyl groups may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group and R 3 is C 1-10 alkyl group which may have any substituent selected from C 1-10 alkoxyl group, halogen atom, cyano group and nitro group; or C 3-8 cycloalkyl group, C 6-10 aryl group or aralkyl group wherein the alkyl moiety is C 1-6 alkyl group and the aryl moiety has 1 to 3 C 6-10 aryl groups, where said cycloalkyl, aryl and aralkyl groups may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group; or C 2-10 alkenyl group which may have any substituent selected from C 1-6 alkoxyl group, halogen atom, cyano group and nitro group, which comprises the steps of (A) reacting a 5-hydroxy-7-oxabicyclo[4.1.0]hept-2-ene-3-carboxylic acid ester of the formula (I) wherein R 1 is as defined above, with an amine of the formula (II) R 2 NH 2 wherein R 2 is a hydrogen atom, or C 1-10 alkyl group which may have any substituent selected from C 1-10 alkoxyl group, halogen atom, cyano group and nitro group; or C 3-8 cycloalkyl group, C 6-10 aryl group or aralkyl group wherein the alkyl moiety is C 1-6 alkyl group and the aryl moiety has 1 to 3 C 6-10 aryl groups, where said cycloalkyl, aryl and aralkyl groups may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group; or C 2-10 alkenyl group which may have any substituent selected from C 1-6 alkoxyl group, halogen atom, cyano group and nitro group, to give a 3-amino-4,5-dihydroxy-1-cyclohexene-1-carboxylic acid ester of the formula (III) wherein R 1 and R 2 are as defined above, (B) protecting an amino group of the obtained 3-amino-4,5-dihydroxy-1-cyclohexene-1-carboxylic acid ester to give a 3-amino-4,5-dihydroxy-1-cyclohexene-1-carboxylic acid ester of the formula (VIII) wherein R 1 and R 2 are as defined above and R 4 is amino-protecting group selected from alkoxycarbonyl group, aralkyloxycarbonyl group, aralkyl group and acyl group, (C) reacting the obtained 3-amino-4,5-dihydroxy-1-cyclohexene-1-carboxylic acid ester with a sulfonylating agent in the presence of a base to give a 3-amino-4,5-disulfonyloxy-1-cyclohexene-1-carboxylic acid ester of the formula (IX) wherein R 1 , R 2 and R 4 are as defined above and A is alkylsulfonyl group wherein the alkyl moiety is C 1-6 alkyl group, which may have any substituent selected from C 1-6 alkoxyl group, halogen atom, cyano group and nitro group; phenylsulfonyl group which may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group on the aromatic ring; or aralkylsulfonyl group wherein the aryl moiety is phenyl group and the alkyl moiety is C 1-6 alkyl group, which may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group on the aromatic ring, (D) removing the amino-protecting group from the obtained 3-amino-4,5-disulfonyloxy-1-cyclohexene-1-carboxylic acid ester to give a 3-amino-4,5-disulfonyloxy-1-cyrlohexene-1-carboxylic acid ester of the formula (X) wherein R 1 , R 2 and A are as defined above, (E) reacting the obtained 3-amino-4,5-disulfonyloxy-1-cyclohexene-1-carboxylic acid ester with a base to give a 5-sulfonyloxy-7-azabicyclo[4.1.0]hept-2-ene-3-carboxylic acid ester of the formula (IV) wherein R 1 , R 2 and A are as defined above, (F) reacting the obtained 5-sulfonyloxy-7-azabicyclo[4.1.0]hept-2-ene-3-carboxylic acid ester with an alcohol of the formula (V) R 3 OH wherein R 3 is as defined above, in the presence of a Lewis acid to give a 5-oxy-7-azabicyclo[4.1.0]hept-3-ene-3-carboxylic acid ester of the formula (VI) wherein R 1 , R 2 and R 3 are as defined above, and (G) when R 2 is a C 1-10 alkyl group which may have any substituent selected from C 1-10 alkoxyl group, halogen atom, cyano group and nitro group; or C 3-8 cycloalkyl group, C 6-10 aryl group or aralkyl group wherein the alkyl moiety is C 1-6 alkyl group and the aryl moiety has 1 to 3 C 6-10 aryl groups, where said cycloalkyl, aryl and aralkyl groups may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group; or C 2-10 alkenyl group which may have any substituent selected from C 1-6 alkoxyl group, halogen atom, cyano group and nitro group, eliminating the 7-position substituent R 2 from the obtained 5-oxy-7-azabicyclo[4.1.0]hept-3-ene-3-carboxylic acid ester (VI). A production method of a 5-oxy-7-azabicyclo[4.1.0]hept-3-ene-3-carboxylic acid ester of the formula (VI) wherein R 1 is C 1-10 alkyl group which may have any substituent selected from C 1-10 alkoxyl group, halogen atom, cyano group and nitro group; or C 3-8 cycloalkyl group, C 6-10 aryl group or aralkyl group wherein the alkyl moiety is C 1-6 alkyl group and the aryl moiety has 1 to 3 C 6-10 aryl groups, where said cycloalkyl, aryl and aralkyl groups may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group, R 2 is a hydrogen atom, or C 1-10 alkyl group which may have any substituent selected from C 1-10 alkoxyl group, halogen atom, cyano group and nitro group; or C 3-8 cycloalkyl group, C 6-10 aryl group or aralkyl group wherein the alkyl moiety is C 1-6 alkyl group and the aryl moiety has 1 to 3 C 6-10 aryl groups, where said cycloalkyl, aryl and aralkyl groups may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group; or C 2-10 alkenyl group which may have any substituent selected from C 1-6 alkoxyl group, halogen atom, cyano group and nitro group and R 3 is C 1-10 alkyl group which may have any substituent selected from C 1-10 alkoxyl group, halogen atom, cyano group and nitro group; or C 3-8 cycloalkyl group, C 6-10 aryl group or aralkyl group wherein the alkyl moiety is C 1-6 alkyl group and the aryl moiety has 1 to 3 C 6-10 aryl groups, where said cycloalkyl, aryl and aralkyl groups may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group; or C 2-10 alkenyl group which may have any substituent selected from C 1-6 alkoxyl group, halogen atom, cyano group and nitro group, which comprises reacting a 5-sulfonyloxy-7-azabicyclo[4.1.0]hept-2-ene-3-carboxylic acid ester of the formula (IV) wherein A is alkylsulfonyl group wherein the alkyl, moiety is C 1-6 alkyl group, which may have any substituent selected from C 1-6 alkoxyl group, halogen atom, cyano group and nitro group; phenylsulfonyl group which may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group on the aromatic ring; or aralkylsulfonyl group wherein the aryl moiety is phenyl group and the alkyl moiety is C 1-6 alkyl group, which may have any substituent selected from C 1-6 alkyl group, C 1-6 alkoxyl group, halogen atom, cyano group and nitro group on the aromatic ring, and R 1 and R 2 are as defined above, with an alcohol of the formula (V) R 3 OH wherein R 3 is as defined above, in the presence of a Lewis acid.

Inventors: ISHIWATA AKIHIRO, TERASHIMA SHIRO, UJITA KATSUJI, ISHIWATA, AKIHIRO, TERASHIMA, SHIRO, UJITA, KATSUJI
Applicants: KURARAY CO, SAGAMI CHEM RES, KURARAY CO., LTD., SAGAMI CHEMICAL RESEARCH CENTER
Application year: 2000
Main classification: C07D203
IPC codes: C07C227, C07D203, C07C229