HUMAN DNA SEQUENCES

Abstract: Novel human cDNA sequence of a clones, the encoded protein sequence of a clones, antibodies and variants thereof, are provided. The disclosed sequence of a clones find application in a number of ways, including use in profiling assays. In this regard, various assemblages of nucleic acids or proteins are provided that are useful in providing large arrays of human material for implementing large-scale screening strategies. The disclosed sequence of a clones may also be used in formulating medicaments, treating various disorders and in certain diagnostic applications.

Description: HUMAN DNA SEQUENCES Background of the Invention Current methods for testing pharmacological substances rely on a three-stage testing approach to drug development. First, candidate compounds are typically screened in some sort of in vitro system, like inhibition of cancer cell growth. Candidates are then tested in an animal model, as a first approximation of systemic effects, including efficacy and toxicity. Compounds that still show promise after these initial in vivo screens, finally are tested in humans. Again, human testing typically occurs in three phases: toxicity; preliminary efficacy; and efficacy. The entire process can take more than a decade and cost hundreds of millions of dollars. Aside from the monetary costs and protracted time scale, moreover, current testing regimes waste the lives of countless laboratory animals and needlessly endanger the lives of human subjects. A need exists, therefore, for more sophisticated drug screening techniques that can be done rapidly in vitro. These screening techniques ideally will be reflective of systemic and/or organ-specific responses, so that they provide a reliable indicator of action in a human body. Current techniques, however, tend to utilize only a single or limited number of markers, thus answering only very simple questions that are of questionable medical import. For example, a typical in vitro assay may ask whether a lead compound binds a particular receptor, which has been implicated in a certain disorder. It is presumed that such binding is indicative of therapeutic usefulness, but it does not even purport to address systemic effects. Not only are screening techniques for efficacy inadequate, the available toxicity screens likewise are inadequate. Toxicity, on a first level, is usually measured by animal testing. Aside from the complications related to in vivo versus in vitro testing, such screens are insufficient because of differences in metabolism, uptake, etc., relative to humans. Thus, improved methods would be not only be in v.trø-based, they would also be more "human. " With the increasing miniaturization of screening assays and the growing availability of targets for pharmaceutical intervention, there is increasing interest in developing arrays containing large numbers of these targets that can be assayed simultaneously. If such an array contains a large enough population of targets, it can be used to essentially mimic the systemic response. In other words, the array becomes an in vitro surrogate for the human body. The more refined the array, the more accurate the predictive capability. In theory, an array could be constructed that can detect all of the known human expression products simultaneously, thereby, providing a very reliable indicator of the human response to a given compound. These arrays offer advantages over the present in vitro screening systems in that they can assay large numbers of responses simultaneously. They are superior to animal testing because they are more "human" and, thus, more predictive of human responses. In order to construct such arrays, however, the field is in need of further human targets. Advantageously, such targets will be provided with additional physiologically relevant information, such as whether the target is expressed in a particular tissue and whether it is related to a known functional class of targets. In this way, the artisan can focus as needed, for example, on tissue-specific effects or target class-specific effects, thereby providing information useful in evaluating efficacy and/or toxicity. In addition to a need for pharmacological screening targets, there is a need for further pharmacological substances. These substances can be used in the formulation of medicinal compositions and in treating a wide variety of disorders. The present invention responds to the aforementioned and other needs in the field by providing a population of novel targets useful, inter alia, in the profiling and medicinal contexts described above. Summary of the Invention It is an object of the invention, therefore, to provide a set of human cDNA clones. Further to this object, the invention provides sequences of human cDNA clones that were isolated from libraries generated from different human tissues. It is another object of the invention to provide assemblages of targets useful in profiling matrices for screening pharmacological test compounds. According to this object, assemblages comprising different populations of human nucleic acids, proteins and antibodies are provided. In different embodiments, cDNA library-specific assemblages and target-family-specific targets are provided. It is a further object of the invention to provide a database of human nucleotide and protein sequences. Further to this object, novel human nucleotide and protein sequences are provided in electronic form. In one embodiment, one or more of these sequences is provided in a searchable database. It is still another object of the invention to provide biologically active target molecules useful in treating or detecting human disorders. Further to this object, the invention provides nucleic acid and protein molecules that have the capacity to affect disease etiology or symptoms or correlate with known disease states. Also further to this object, a database is provided which comprises the disclosed molecules in electronic form. It is still a further object of the invention to provide polypeptides encoded by the human cDNA clones disclosed herein. Further to this object, the invention provides antibodies and fragments thereof that are capable of binding to a specific portion of these polypeptides. It is yet another object of the invention to provide pharmaceutical compositions which comprise an effective amount of a pharmaceutical agent, wherein the pharmaceutical agent is selected from the group consisting of one or more polypeptides contemplated by the invention, variants or functional derivatives thereof, and antibodies thereto; and a physiologically acceptable carrier or excipient. It is still another object of the invention to provide expression vectors comprising one or more human cDNA clones disclosed herein or fragments thereof; and optionally a promoter operably linked to the cDNA clone or fragment thereof . Further to this object, the invention provides methodology for recombinanfly producing a desired peptide, comprising expressing in a host cell a peptide encoded by a human cDNA clone disclosed herein. Detailed Description The invention results from a need in the art for new human nucleic acids and proteins. This need arises in several contexts. First, there is a need to identify targets for therapeutic intervention. Second, there is a need to identify molecules that may be adversely affected in a therapeutic context, thereby resulting in toxicity. Knowledge of these molecules will aid in the design of new medicaments with enhanced efficacy and decreased toxicity. Finally, the need encompasses human nucleic acids and proteins that have medicinal applicability in their own right. In view of these needs, the present inventors set out to isolate and sequence human cDNAs from tissue-specific libraries. In this way, they represent subsets of molecules likely to be targets for therapeutic intervention or for avoiding toxicity. In addition, the inventors divided the molecules into various sub-categories, based on suspected functionality, structural similarity etc, which are of interest from a pharmacological perspective. These molecules are disclosed in provisional application serial nos. 60/149,499 and 60/156,503, filed August 18, 1999, and September 28, 1999, respectively, both of which are hereby incorporated by reference in their entirety. GENERAL DESCRIPTION OF THE INVENTIVE MOLECULES The present invention provides novel polynucleotide molecules that, in some instances, have similarities with known molecules. The inventive DNAs were cloned from five different human cDNA libraries. In addition to these DNA molecules, the invention provides their protein translations and antibodies derived from them. The inventive DNA and protein sequences are show individually, below. The inventive nucleic acids also include the complements of these DNA sequences, as well as their RNA counterparts. Methods of producing the molecules also are provided. Further, the invention provides methods for detecting all or part of the molecules and of detecting polynucleotides encoding all or part of the molecules. The inventive molecules derive from five cDNA libraries: human fetal brain; human fetal kidney; human mammary carcinoma; human testis; and human uterus. For convenience, each sequence bears a designation that indicates from which library it is derived. In particular, these designations are: "hfpbr" for human fetal brain; "hfkd" for human fetal kidney; "hmcf" for human mammary carcinoma; "htes" for human testis; and "hute" for human uterus. The individual libraries were constructed and screened as described below in the examples. The protein and DNA molecules of the invention are variously described herein as "target" molecules or "inventive" molecules. The sequences and other information pertinent to the nucleic acid and protein molecules of the invention are shown, below. Interpreting the data disclosed with the Table and cDNA sequences, below: The table and data below provide the coding sequences of the inventive cDNAs as well as the protein sequences and other useful information, as set out below. Grouping The clones were assigned to the following fourteen functional and/or tissue-derived groups: 1. Cell Cycle 2. Cell Structure and Motility 3. Differentiation/Development 4. Intracellular Transport and Trafficking 5. Metabolism 6. Nucleic Acid Management 7. Signal Transduction 8. Transmembrane Protein 9. Transcription Factors 10. Brain derived 11. Kidney derived 12. Mammary Carcinoma derived 13. Testes derived 14. Uterus derived Description of Clone Files The individual clone files are structured in the same pattern. The Sections are separated by paragraphs. 1. Clone Name The clone names are deciphered with reference to the following example: DKFZphfkd2_24e23, wherein the code represents: • producer of library ("DKFZ") (for convenience, this reference may be eliminated) • a "p" for "plasmid cDNA library" (for convenience, this reference may be eliminated) • library name (e.g. hfbr = human fetal brain; hfkd = human fetal kidney; hmcf = human mammary carcinoma; htes = human testes; hute = human uterus) an underscore ("_") to separate library information from plate information plate number (e.g. "16") plate coordinates (letter first; e.g. "fl4") Group 3. Introduction short review of the similarities, function of the protein and possible applications 4. Short Information specifications about the cDNA (who sequenced, completeness of the cDNA, similarity, who sequenced, chromosomal localisation, length of cDNA, localisation of poly A tail and polyadenylation signal) 5. cDNA-Sequence 6. BLASTn Results search results of blasting the cDNA sequence against all public databases 7. Medline Entries information about genes/proteins similar to the novel cDNA (if available) 8. Putative Encoded Protein Information specifications about the encoded protein (ORF: length and localisation of the reading frame) 9. Protein Sequence 10. BLASTp Results search results of blasting the protein sequence against all public databases 11. Pedant Information output of fully automated annotation: summarises peptide information, homologies, patterns as follows: [Length] - length of the protein = number of amino acid residues [MW] - molecular weight of the protein [pi] - isoelectric point [HOMOL] - shows protein with closest similarity to the cDNA-encoded protein [FUNCAT] - functional information according to a catalogue developed by Munich Information center for Protein Sequences (MIPS) [BLOCKS] - Blocks are multiply aligned ungapped segments corresponding to the most highly conserved regions of proteins. The blocks for the Blocks Database are made automatically by looking for the most highly conserved regions in groups of proteins documented in the Prosite Database. The Prosite pattern for a protein group is not used in any way to make the Blocks Database and the pattern may or may not be contained in one of the blocks representing a group. These blocks are then calibrated against the SWISS-PROT database to obtain a measure of the chance distribution of matches. It is these calibrated blocks that make up the Blocks Database. The WWW versions of the Prosite and SWISS-PROT Databases that are used on this server are located at the ExPASy World Wide Web (WWW) Molecular Biology Server of the Geneva University Hospital and the University of Geneva. World Wide Web URL http://blocks.fhcrc.org/blocks/about_blocks.html/ is the entry point to the database. - here Blocks segments found in the analysed protein sequences are displayed [SCOP] Nearly all proteins have structural similarities with other proteins and, in some of these cases, share a common evolutionary origin. The scop database provides a detailed and comprehensive description of the structural and evolutionary relationships between all proteins whose structure is known, including all entries in Brookhaven National Laboratory's Protein Data Bank (PDB). It is available as a set of tightly linked hypertext documents which make the large database comprehensible and accessible. In addition, the hypertext pages offer a panoply of representations of proteins, including links to PDB entries, sequences, references, images and interactive display systems. World Wide Web URL http://scop.mrc-lmb.cam.ac.uk/scop/ is the entry point to the database. Existing automatic sequence and structure comparison tools cannot identify all structural and evolutionary relationships between proteins. The scop classification of proteins has been constructed manually by visual inspection and comparison of structures, but with the assistance of tools to make the task manageable and help provide generality. Proteins are classified to reflect both structural and evolutionary relatedness. Many levels exist in the hierarchy, but the principal levels are family, superfamily and fold. The exact position of boundaries between these levels are to some degree subjective. Scop evolutionary classification is generally conservative: where any doubt about relatedness exists, we made new divisions at the family and superfamily levels. - - here SCOPE segments found in the analysed protein sequences are displayed [EC] ENZYME is a repository of information relative to the nomenclature of enzymes. It is primarily based on the recommendations of the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology (IUBMB) and it describes each type of characterized enzyme for which an EC (Enzyme Commission) number has been provided. World Wide Web URL http://www.expasy.ch/enzyme/ is the entry point to the database. - here EC -number and name of enzymes with similarity to the analysed protein sequences are displayed [PIRKW] - functional information according to the Protein Information Resource (PIR) database catalogue developed by Munich Information Center for Protein Sequences (MIPS), the National Biomedical Research Foundation (NBRF) and the International Protein Information Database in Japan (JIPID). [SUPFAM] - information according to the Protein Information Resource (PIR) database catalogue of protein superfamilies developed by Munich Information Center for Protein Sequences (MIPS), the National Biomedical Research Foundation (NBRF) and the International Protein Information Database in Japan (JIPID). [PROSITE] please refer to 12. PROSITE Motifs [PFAM] please refer to 13. PFAM Motifs [KW] - overall 2dimensional folding information - 3D indicates that the proteins is similar to a protein of which a 3 dimensional structure is known - overall structural information G The last PEDANT-block depicts information about the folding structure of the protein generated by PREDATOR. PREDATOR is a secondary structure prediction program. It takes as input a single protein sequence to be predicted and can optimally use a set of unaligned sequences as additional information to predict the query sequence. The mean prediction accuracy of PREDATOR is 68% for a single sequence and 75% for a set of related sequences. PREDATOR does not use multiple sequence alignment. Instead, it relies on careful pairwise local alignments of the sequences in the set with the query sequence to be predicted. World Wide Web URL http://www.embl- heidelberg.de/argos/predator/predator_info.html is the entry point to the database. - H = helix, E - extended or sheet, _ = coil, T = transmembrane, B = beta - x indicates a low-complexity region with repeat-like structure which is omitted in all BLAST searches 12. PROSITE Motifs PROSITE is a database of protein families and domains. It consists of biologically significant sites, patterns and profiles that help to reliably identify to which known protein family (if any) a new sequence belongs. World Wide Web URL http://www.expasy.ch/prosite/ is the entry point to the database. A description of the prosite consensus patterns is also provided, below. 13. PFAM Motifs PFAM (protein families) is a large collection of multiple sequence alignments and hidden Markov models covering many common protein domains. World Wide Web URL http://www.sanger.ac.uk/Pfam/ is the entry point to the database. Deposit of Clones Clones were deposited as a pool with the American Type Culture Collection under accession number , from which each clone comprising a particular polynucleotide is obtainable. Each clone has been transfected into separate bacterial cells (E. coli) in this composite deposit. The clones may also be obtained from the Resource Center of the German Human Genome Project (Heubner Weg 6, 14059 Berlin, GERMANY). The Resource Center library numbers are slightly different that those presented here, but may be readily obtained by the following key or with the assistance of Resource Center personnel. The library name becomes a number: brain (hfbr2) becomes 564; kidney (hfkd2) becomes 566; mammary carcinoma (hmcfl) becomes 727; testis (htes3) becomes 434;and uterus (hutel) becomes 586. Next, the plate number is converted to two digits (e.g., "2" becomes "02") and is moved behind the plate coordinate, and the underscore is dropped. The following examples are helpful: Listed Number Resource Center Number DKFZphfbr2_l 6f21 DKFZp564F2116 DKFZphfkd2_lj9 DKFZp566J091 DKFZphmcfl_lc23 DKFZp727C231 DKFZphtes3_14g5 DKFZp434G0514 DKFZphutel_17k7 DKFZp586K0717 The libraries were constructed using two commercially available vectors. The brain (hfbr2 designations) and kidney (hfkd2 designations) libraries utilize pAMP 1 from Life Technologies and are maintained in XL-2Blue (Strategene); the uterus (hutel), testes (htes3) and mammary carcinoma (hmcfl) libraries are constructed in pSPORTl, also from Life Technologies, and are maintained in DH10B (LifeTechnologies). In addition to the following techniques, consultation with the commercial literature available on these clones will make evident all of the housekeeping techniques needed to propagate and isolate the individual constructs. All inserts may be excised with a Notl/Sall digestion. Alternatively, universal primers, flanking the cloning region, may be used to amplify the inserts using PCR methods. Bacterial cells containing a particular clone can be obtained from the composite deposit as follows: An oligonucleotide probe or probes should be designed to the sequence that is known for that particular clone. This sequence can be derived from the sequences provided herein, or from a combination of those sequences. Methods of probe design are presented below. Oligonucleotide probes may be labeled with γ- 2 P ATP (specific activity 6000 Ci/mmole) and T4 polynucleotide kinase using commonly employed techniques for labeling oligonucleotides. Other, non-radioactive labeling techniques can also be used. Unincorporated label typically is removed by gel filtration chromatography or other established methods. The amount of radioactivity incorporated into the probe can be quantified by measurement in a scintillation counter. Preferably, specific activity of the resulting probe generally should be approximately 4X10 6 dmp/pmole. The bacterial culture containing the pool of full-length clones should preferably be thawed and 100 μl of the stock used to inoculate a sterile culture flask containing 25 ml of sterile L-broth containing ampicillin at 50 - 100 μg/ml (for XL-2Blue strains 25 μg/ml tetracycline should also be used). The culture should preferably be grown to saturation at 37°C, and the saturated culture should preferably be diluted in fresh L-broth. Aliquots of these dilutions should preferably be plated to determine the dilution and volume which will yield approximately 5000 distinct and well-separated colonies on solid bacteriological media containing L-broth containing ampicillin at 100 μg/ml (for XL-2Blue strains 25 μg/ml tetracycline should also be used)and agar at l.5% in a l50 mm petri dish when grown overnight at 37°C. Other known methods of obtaining distinct, well-separated colonies can also be employed. Standard colony hybridization procedures should then be used to transfer the colonies to nitrocellulose filters and lyse, denature and bake them. The filter is then preferably incubated at 65°C. for 1 hour with gentle agitation in 6 x SSC (20 x stock is 175.3 g NaCl/liter, 88.2 g Na citrate/liter, adjusted to pH 7.0 with NaOH) containing 0.5% SDS, 100 μg/ml of yeast RNA, and 10 mM EDTA (approximately 10 mL per 150 mm filter). Preferably, the probe is then added to the hybridization mix at a concentration greater than or equal to 1X10 6 dpm/mL. The filter is then preferably incubated at 65°C. with gentle agitation overnight. The filter is then preferably washed in 500 mL of 2 x SSC/0.5% SDS at room temperature without agitation, preferably followed by 500 mL of 2 x SSC/0.1% SDS at room temperature with gentle shaking for 15 minutes. A third wash with 0.1 x SSC/0.5% SDS at 65°C. for 30 minutes to 1 hour is optional. The filter is then preferably dried and subjected to autoradiography for sufficient time to visualize the positives on the X-ray film. Other known hybridization methods can also be employed. The positive colonies are picked, grown in culture, and plasmid DNA isolated using standard procedures. The clones can then be verified by restriction analysis, hybridization analysis, or DNA sequencing. Alternatively, clones may be grown as described above, and PCR used to isolate the insert DNAs. Methods of PCR are described below and are otherwise well known . ERROR SCREENING The DNA sequences found herein derive from individual clones, which are publicly available, as noted above. Thus, the skilled artisan will recognize that any specific sequence disclosed herein readily can be screened for errors by resequencing a particular fragment, in both directions (i.e. , by sequencing both strands). Alternatively, error screening can be performed by amplifying and/or cloning any of the inventive DNAs, using for example RT- PCR, and sequencing the resulting amplified product. In the event that there is a sequencing error, reference should be made to the deposited clone as the correct sequence. USES AND BIOLOGICAL ACTIVITIES OF THE INVENTIVE MOLECULES The inventive molecules and their derivatives are susceptible to a wide variety of uses, based on functional and/or structural properties. The skilled worker will appreciate, based on the biological activities detailed below, and discussed with regard to the individual sequences disclosed below, that the inventive molecules will find usefulness in numerous therapeutic and diagnostic applications. The DNA molecules, especially the potassium salts thereof, can be used as fertilizer supplements due to their high nitrogen and phosphorus contents. Since the DNAs are of defined length, they are also useful in gel electrophoresis as molecular weight markers. Due to their similarity with known molecules, certain of the DNA molecules and their variants and derivatives may be used in any number of different diagnostic procedures and therapeutic applications. They may also be used to make the encoded proteins. The proteins themselves have many possible uses. They may be used as a nutritional supplement for humans, animals and even for laboratory use as, for example, medium for bacterial cultures. Moreover, since the proteins are of defined, known sizes, they may be used as molecular weight markers for gel electrophoresis and gel filtration. Because they are of defined sequences, they also have use in microsequencing and protein fingerprinting applications. Expression Profiling Applications Given their known tissue expression and functional associations, assemblages of the inventive proteins (or corresponding antibodies) and nucleic acids are particularly suited to expression profiling applications. Expression profiling generally entails constructing an array of indicators that signal the presence of a particular RNA or protein expression product. Such arrays can be used to evaluate, for example, pharmacological effectiveness and toxicity. In particular, expression profiles from such arrays can be generated from cells treated with known compounds, having known properties, and these profiles can be compared to profiles of unknowns to evaluate similarities and differences, which can be correlated with efficacy or toxicity. Additional uses of profiling include diagnosis, tracking development, and ascertaining signaling and metabolic pathways. For examples of references describing profiling and its uses, see Farr et α/., U.S. Patent 5,811,231 (1998); Seilhamer et al. , U.S. Patent 5,840,484 (1998); Rine et al, U.S. Patent No. 5,777,888 (1998); WO 97/27317; WO 99/05323; WO 99/09218; and WO 99/14369. For a device for implementing such techniques, see Lipshutz et al , U.S. Patent No. 5,856,174 (1999) and Anderson et al., U.S. Patent No. 5,922,591 (1999). In one embodiment, a subset of the inventive DNAs will be arrayed on a substrate, like a gene chip, a filter or a 96-well plate. Test samples containing cells are maintained in the presence of a label capable of incorporation into nascent mRNA. Samples are treated with test and control compounds, which will induce mRNA expression in the sample, resulting in incorporation of label. Whole mRNA is isolated and applied to the array such that it hybridizes with the DNAs contained therein. After washing, the amount of hybridization is quantified and a profile is generated. These steps are repeated with various control and test compounds, thereby generating a library of profiles, which can be used to ascertain the relationships relevant to pharmacological efficacy or toxicity. The matrices used in such profiling, however, need not be limited to those utilizing DNAs. Rather, other nucleic acids, like RNAs and protein nucleic acids (PNAs), as well as the inventive proteins and antibodies corresponding to the inventive proteins may also be employed. Hence, for example, antibodies could form the array and the samples could be treated in order to label nascent proteins. Whole proteins then would be isolated and applied to the antibody matrix. Developing the resulting signal would result in a protein expression profile, which is useful in essentially the same manner as the nucleic acid profile. A protein matrix could be used, for example, in evaluating antibody responses to pharmaceutical agents in order to eliminate possible cross-reactivity. Moreover, where nucleic acids are used in the matrix, it is often beneficial to use variants (as defined below) of the molecules described herein. This can be used to account for genetic variations that are of little or no consequence to the function of the resultant gene product. Hence, they can account for wobble or conservative amino acid variations that do not perturb function, like variations in some of the protein motifs elucidated below. Thus, each position in the matrix can employ multiple nucleic acid probes that account for a series of variants. Expression profiling may also be done, in another embodiment, using two- dimensional protein gels in which the inventive proteins are detected. The resultant profiles can be used in the same way as described. Matrices useful for profiling may be constructed based on different criteria. Of course, the more relevant profiles will take into account expression of most human genes, preferably all of them. In certain situations, however, it is advantageous to look at a smaller subset. For example, if one were concerned about fetal neural toxicity, a fetal brain-specific matrix might be chosen. On the other hand, if one were interested in targeting mammary carcinoma tissue, a corresponding matrix could be used. Thus, matrices may be constructed using all of the sequences available from a tissue-specific library. * * * The following discussion relates to some of the various functional and structural groupings that would be of interest to the artisan wishing to construct profiling matrices. Of course, the artisan will also recognized that these functional descriptions may find additional applicability in the therapeutic and diagnostic applications discussed below. Cell Cycle A proliferating cell must coordinate replication and chromosomal separation to ensure that the genome is replicated completely, and that a single copy, is correctly inherited by each daughter cell. The cell cycle is the coordinated series of events that achieves these aims. Many of the key events are initiated by a family of conserved Seiren/threonine protein kinases, the cyclin-dependent kinases (CDKs), that are activated by the cyclin family of proteins (cyclins A-H). In turn, the cyclin-CDK complexes are modulated by other protein kinases or phosphatases, and by binding specific inhibitor proteins. The enormous variety of ways in which CDK activity can be regulated allows the cell to respond to internal signals generated by preceding events in the cell cycle and to external growth signals. The somatic cell cycle is divided into four phases: DNA replication (S phase) and chromosome separation (M phase) are separated by gap phases (GI and G2). At specific control points the decision to begin the next stage (DNA synthesis or mitosis) is carefully regulated. Cdc2, the primary kinase, is especially required for the Gl-S transition and S phase. Cdc4 and Cdc6 are involved at the restriction point, where the cell can decide to proliferate or arrest (GK->G0) and Cdc7 is a CDK activating kinase (CAK) as well as a subunit of TFIIH. The Cyclin-CDK complexes are regulated in various ways. One is through phosphorylation by CDK activating kinases (CAK), like the Y15 kinase (Weel) and dephosphorylation by CDK associated phosphatases (CAP), like Cdc25A a member of the Cdc25 family (Cdc25A, B and C). An other way of regulation occurs through two classes of CDK inhibitors (CKI), the INK4 proteins pi 5, pi 6, pi 8, and pi 9, who negatively regulates the cyclin D CDK complexes and second the p21 family with p21, p27, and p57. The cell cycle is also regulated through ubiquitin-mediated proteolysis involving the destruction of both cyclins and CDK inhibitors by the 26S proteasome, that requires an ubiquitin conjugating enzyme (UBC) and an ubiquitin ligase. The instability is conferred by PEST regions (cyclin D and E) or a ten amino acid region in the amino terminus (degradation box) in the A- and B-type cyclins. All these modifications play an important role for the cellular localization, because only the nuclear CDK-cyclin complexes are functional for cell cycle. During GI phase of the cell cycle, cyclines A, E and D are synthesized and bind to their cyclin-dependent kinase (CDK) partners. CDK complexes containing cyclins A, E and Dl are then imported into and concentrated within nuclei. Cdk6- cyclin D3 has been localized to both cytoplasmic and nuclear compartments, although only the nuclear complex is active. As cells enter S phase, cyclin A and cyclin E complexes remain within the nucleus, whereas cyclin Dl relocalizes to the cytoplasm for proteolysis at the onset of S phase. Like Cdk2-cyclin A, Cdc2-cyclin A is nuclear and remains so until it is degraded during mitosis. By contrast, as a result of ongoing nuclear import and more rapid re-export, cyclin Bl, which binds to Cdc2 upon synthesis during S phase, is predominantly cytoplasmic. Cdc2-cyclin B2 is also cytoplasmic, although this might occur through anchoring of the complex to some cytoplasmic constituent. At prophase, phosphorylation of cyclin Bl promotes accumulation of Cdc2 -cyclin Bl in the nucleus, whereas cyclin B2 remains in the cytoplasm until nuclear envelope breakdown. Two crucial regulators of Cdc2-cyclin B-Weel and Cdc25C exist and are responsible for the G2 to M control point. Weel is a nuclear protein throughout the cell cycle, whereas Cdc25C binds to 14-3-3 proteins during interphase and remains predominantly cytoplasmic. In some systems Cdc25C, like cyclin Bl, rushes precipitously into the nucleus just before entry into mitosis. The 110-kDa retinoblastoma (tumor suppressor) protein (RB), a pRB-family member is an important regulator of cell-cycle progression and differentiation. Like the E2F family (E2F1-5) or DP family (DP 1-3) of transcription activators, RB suppresses inappropriate proliferation by arresting cells in GI by repressing the transcription of genes required for the transition into S phase. Before the cell proceeds into S phase, RB becomes phosphorylated at multiple sites by the cyclin dependent protein kinases (CDKs) and loses its transcriptional repressing activity. Phosphorylation of RB during late GI phase results in the dissociation of the E2F-RB repressor complex which allows S-phase specific genes to be transcribed. Cyclin E is the evolutionary conserved target for E2F and interacts together with CDC2 in late GI . For a proliferating cell it is vital that only undamaged DNA is replicated because if DNA damage is substantial, its replication can lead to chromosome loss or rearrangement. Thus, we find a G1<->S checkpoint in late GI that requires tumor suppressor p53. A p53- dependent GI arrest is effected by the cyclin dependent kinase inhibitor p21 through higher expression levels that inhibits almost all cyclin CDK complexes. The kinase responsible for phosphorylating the unidentified kinetochore component in metaphase may be a member of the MAP kinase family and appears to be the proto oncogene c-MOS, a cytostatic factor (CSF) in meiosis. Several categories of proteins are coded for by clones of the invention within the overall group of "Cell cycle"and include, among others, the following: Tumor suppressors (e.g. N33): Tumour-suppressor genes are known to be involved in the control of cell growth and division, interacting with proteins which control the cell cycle. The N33 gene is significantly methylated in tumour cells, a mechanism by which tumor- suppressor genes are inactivated in cancer. The N33 gene has been reported by OMIN OMIN (Online Mendelian Inheritance in Man at http://www.ncbi.nlm.nih.gov/htbin-post/Omin) to be associated (as potentially diagnostic, therapeutic, causative, and or related, etc..) with the following diseases: 1) prostate cancer suppression (OMIN *601385). Clones in this category include: fbr2_2kl4. C-TAK1 Cdc25c associated protein kinase: Cdc25C is a protein kinase that controls entry into mitosis by dephosphorylation of Cdc2. Cdc25C function is regulated by phosphorylation, too. Serine 216 phosphorylation of Cdc25C mediates the binding of 14-3-3 protein to Cdc25C. C-TAK1 (Cdc twenty-five C associated protein kinase) phosphorylates Cdc25C on serine 216 in vitro. Alterations in the gene coding for the above protein kinase has been reported by OMIN to be associated (as potentially diagnostic, therapeutic, causative, and or related, etc...) with Pancreatic cancer (OMIN *60278). Clones in this category include: tes3_7j3. Cell structure and motility One of the major differences between prokaryotes and eukaryotes is the ability of the eukaryotic cell to adopt very different shapes dependent on its function during the differentiation process. Animal cells vary from being round to extended cylindric forms like motorneurons or muscle cells. In humans, more than 100 different cell types can be distinguished, each having a characteristic shape. The form of a cell often is closely related to its capacity to move. Some completely differentiated cells like fibroblasts can still change their form actively, thereby migrating. Other cell types serve as motor elements - "macroscopically" like muscle cells or "microscopically" like ciliated epithelia. Such tasks are fulfilled by a big class of proteins; on the one hand responsible for maintenance of cell structure and contacting neighbor cells or the intercellular matrix and on the other hand for cell motility. These topics cannot be regarded separately: The motility apparatus e.g. must be fixed in the cytoskeleton. Three different types of filaments can be distinguished: Actin filaments, tubulin filaments and intermediate filaments, each present in almost all types of cells. Actin filaments (F-actin) are built up of monomers (G- Actin). In muscle cells, actin, myosin, for both of which several paralogous genes are known, as well as many more proteins are constituents of the contractile apparatus. The "thin" and "thick filaments" in a muscle cell consist mainly of actin and myosin, respectively. Several different proteins are responsible for the anchoring of the actin filaments in the Z-disks (e.g. alpha-actinin and desmin) or at the end of the myofibers in the cell membrane. Troponin I, -C, -T and Tropomyosin - associated with actin - confer the Ca++- dependent triggering of contraction. Length of the sarcomere is controlled by the giant protein titin. In smooth muscle, there is no troponin. Contraction activity is controlled by phosphorylation / dephosphorylation of myosin by a specialized kinase instead. Contractile fibers are not organized in sarcomeres. Apart from contributing to muscle contraction, the actomyosin system is responsible for many other motions at cellular level, e.g. the amoeboid movement of pseudopodia or the fission of cells at the end of mitosis by a contractile ring. Besides this, actin fibers fulfill structural tasks like maintenance of the shape of stereocilia or microvilli. Here, actin filaments are connected by proteins like fimbrin. But not only specialized structures like the mentioned ones contain actin fibers. There is a network covering the complete cell volume with F-actin as a major constituent. Whereas the actin filaments in the structures mentioned above are relatively stable, this F-actin is highly dynamic. Management of the network structure and turnover is achieved by connecting proteins like alpha-actinin, fimbrin or fill-in; turnover is regulated by gelsolin, villin, and different capping- and fragmentation-proteins. Microtubules are built up of alpha-beta tubulin heterodimers. Turnover of filaments is achieved by building-in and releasing of monomers with different time constant rates at both ends. The resulting cycle is called "treadmilling". Thirteen strings of tubulin duplets build up one subfiber, whereas one fiber contains two or three of those. A complete axoneme consists of 9 radial and 2 central fibers. This "9+2" - structure is the basis both of flagella, their basal bodies and centrioles. In flagella, several additional structures like radial elements exist. Nexin connects the fibers and dyneine is the motor ATPase which shifts the fibers relative to each other. Several genetic diseases like the Cartageneric syndrome are caused by deficiencies of distinct proteins in cilia. Besides this, microtubules are abundant in all types of cells. They are part of a delivery system for organelles, e.g. in the golgi apparatus. A further very important system based on microtubules is the mitotic spindle, it is organized by the centrosomes. Besides many other components, the major part of a centrosome are two centrioles which are built up of nine microtubule-triplets. Most remarkably, new centrioles are not synthesized de novo but generated by duplication of old ones. Cytoplasmic microtubules are associated with many different proteins. Two major classes are known: The MAPs ("microtubule-associated proteins", with molecular masses between 200 and 300 kD) and the much smaller tau-Proteins with a MW between 60 and 70 kD. These proteins regulate the treadmill-process and the interaction with other structures in the cell. Besides actin and myosin the so-called intermediate filaments constitute a third class of filaments. In contrast to the former two groups, they do not participate in motility, nor are they dynamic structures subject to a vivid turnover. The most important ones are neurofilaments (in neurons), keratin filaments (mainly in epithelial cells), and vimentin filaments (in many sorts different cell types). The biological function of both the cytoskeleton as well as contractile apparatus of a cell does not end at the cell membrane. Cells must be embedded in the extracellular matrix, all cells of a muscle must act as one single mechanical unit and epithelia must resist macroscopic mechanical forces. Hence, cell adhesion and the extracellular matrix are closely connected to the cytoskeleton. Vincullin is one of the proteins which serve as an anchor for intracellular fibers (actin). Different types of desmosomes and tight junctions connect neighbor cells with intercellular fibers. On the inside, cytoplasmic plaques connect them to the cytoskeleton. These structures, on the one hand, serve as mechanical elements whereas gap junctions, on the other hand, connect cells metabolically. The extracellular matrix consists of a network of proteins, glycoproteins and polysaccharides. Different proteins are present in relation to different mechanical demands:. Elastin is found in tissues with high elasticity (lungs, heart) whereas collagen, a more hard- wearing protein, is found in tendons and ligaments. Fibronectin is an extracellular protein highly important for cell adhesion. Reference: Murray J et al (1992): Cell Motil Cytoskeleton 22: 211-223. Within the overall group of Cell Structure and Motility several categories of proteins are coded for by clones of the invention: Collagen alpha chain proteins: Proteins with the typical (xxG)n repeat of collagen proteins and Pfam von Willebrand factor type A domain(s) suggest they are collagen alpha chains. These proteins can find application in modulation of connective tissue, bone and cartilage development and maintainance. OMIN reports collagen alpha chains have associations (as potentially diagnostic, therapeutic, causative, and/or related, etc..) with the following diseases: 1) Osteogenesis imperfecta, type I (OMIN #166200); 2) Osteogenesis imperfecta congenita (OMIN #166210); 3) Alport Syndrome, X-linked (OMIN #301050); 4) Thrombastenia of Glanzmann and Naegeli (OMIN *273800); 5) Ehlers-Danlos Syndrome, Type VII (OMIN #130060); 6) Marfan Syndrome (OMIN #154700); 7) Alport Syndrome, Autosomal Recessive (OMIN #203780); 8) Alpha-2-Deficient Collagen Disease (OMIN 203760); 9) Goodpasture Syndrome (Omin 233450); 10) Osteogenesis Imperfecta, progressively deforming, with normal sclerae (OMIN #259420); 11) ) Ehlers-Danlos Syndrome, Type VII Autosomal Recessive (OMIN *225410); and 12) ) Osteogenesis imperfecta, Type IV (OMIN #166220). OMIN reports that von Willebrand factor type A domains have associations (as potentially diagnostic, therapeutic, causative, and/or related, etc..) with the following diseases:: 1) Hemophilia A (OMIN *306700); 2) Von Willebrand Disease (OMIN * 193400); 3) Giant Platelet Syndrome (OMIN *231200); 4) Thrombastenia of Glanzmann and Naegeli (OMIN *273800); 5) Congenital Thrombotic Diseasae due to protein C deficiency (OMIN #176860); 6) Polycystic Kidney Disease 1 (OMIN *601313); 7) Nephrogenic Diabetes Insipidus (OMIN *304800); 8) Factor V Deficiency (OMIN *227400); and 9) Dentatorubral-Pallidoluysian Atrophy (Omin * 125370). Clones in this category include: fbr2_2b5. Radial spokehead protein: Radial spokehead proteins, e.g., Chlamydomonas reinhardtii radial spokehead protein of flagella or axoneme and the Strongylocentrotus purpuratus sea urchin spermatozoa protein p63, and human proteins with similarity thereto are important for the maintenance of a planar form of sperm flagellar beating. The human protein(s) can find application in modulating the structure of the human spermatozoa radial spoke head and modulation of sperm motility in men (e.g., in sterility). Clones in this category include: tes3_15i5. Ankyrins: Ankyrins are peripheral membrane proteins which interconnect integral proteins with the spectrin-based membrane skeleton. Thus these proteins are involved in coupling of cyto skeleton and cell membrane. OMIN reports that Ankyrins have associations (as potentially diagnostic, therapeutic, causative, and/or related, etc...) with the following diseases: 1) Heriditary Spherocytosis (OMIN * 182900); 2) Hemolytic Poikilocytic Anemia due to reduced ankyrin binding sites (OMIN 141700); 3) Atypical Elliptocytosis (OMIN 225450); 4) Autosomal recessive spherocystosis (OMIN #270970); 5) Werner Syndrome (OMIN *277700); and 6) Rhesus-unlinked type Elliptocytosis (OMIN #130600). Clones in this category include: tes3_l 817. FGD1 -related F-actin binding protein (Farbin/FGDl): FGD1 -related F-actin-binding protein (Farbin/FGDl) is a novel F-actin-binding protein. The gene locus fgdl seems to be responsible for faciogenital dysplasia or Aarskog-Scott syndrome. (OMIN 305400). Frabin binds F-actin and shows F-actin-cross-linking activity. Overexpression of frabin in Swiss 3T3 cells and COS7 cells induces cell shape change and c-Jun N-terminal kinase activation, as described for FGD1. Because FGD1 has been shown to serve as a GDP/GTP exchange protein for Cdc42 small G protein, it is likely that frabin is a direct linker between Cdc42 and the actin cytoskeleton. Cdc42p is an esin yeast, Cdc42p transduces signals to the actin cytoskeleton to initiate and maintain polarized growth and to mitogen-activated protein morphogenesis. In mammalian cells, Cdc42p regulates a variety of actin-dependent events and induces the JNK/SAPK protein kinase cascade, which leads to the activation of transcription factors within the nucleus. Clones in this category include: tes3_72kl5. Paramyosins: Paramyosin is a major structural component of thick filaments and invertebrate muscle. Paramyosins are promising antigens for immunization against several parasites, such as Schistosoma mansoni. Clones in this category include: tes3_7b22. Tuftelin: Tuftelin/enamelin are matrix proteins of the teeth. As other proteins involved in calcification, these proteins are also expressed in the uterus matrix. The new protein can find application in modulation of tissue-calcification, especially the uterus. As reported by OMIN, tuftelin has been associated (as potentially diagnostic, therapeutic, causative, and/or related, etc...) with amelogenesis imperfecta (OMIN *600087). Clones in this category include: utel_19g22. Cell Adhesion Regulator (CARD: CAR1 is involved in the regulation of cell-cell adhesion. OMIN reports the association (as potentially diagnostic, therapeutic, causative, and/or related, etc...) of CAR1 with tumor suppression by the reduction of tumor invasion (OMIN *116935). Clones in this category include: utel_24j6. Differentiation Development Almost every multicellular organism originates from meiotic cell divisions and the recombination of a paternal and a maternal set of chromosomes. After fertilization of the egg, all cells of a body originate from this one cell. Thus the cells of the developing body are initially genetically alike. But phenotypically they become very different. They are specialized to a certain cell type and arranged in an organized pattern to a certain type of tissue and the whole structure has the well-defined shape of an organ. All these features are determined by the DNA sequence of the genome, which is reproduced in every cell. Each cell acts on the genetic instructions given to a certain time and at a certain place of development and plays its individual part in the multicellular organism. Cell differentiation may be divided into three general steps: cell cycle exit, apoptosis protection and tissue specific gene expression. These processes are coordinated to provide the final and unique tissue characteristics. An animal cell that has achieved a certain level of development is said to be determined. This differentiation of a cell may be irreversible and in that case the cell may be renewed only by simple duplication. Other cells are renewed by means of stem cells which are immortal ( e.g. stem cells of the bone marrow, epidermal stem cells). The genetic control of development is extensively studied in non- vertebrates and vertebrates. The classical animal model is the fruit fly Drosophilia and the modern model is the transgenic mouse. Animal transgenesis has proven to be useful for physiological as well as physiopathological studies. Besides the approach based on the random integration of a DNA construct in the mouse genome, gene targeting can be achieved using totipotent embryonic stem cells for targeted transgenesis. Transgenic mice are than derived from the embryonic stem cells. This allows the introduction of null mutations in the genome (so-called knock-out) or the control of the transgene expression by the endogeneous regulatory sequence of the gene of interest (so- called knock-in). Mice can be created that express wild-type genes, mutant genes, marker genes or cell lethal genes in a tissue specific manner. These animal models allow to follow changes in tissue and organ development and lead to a better understanding of the cellular function of many genes or to the generation of animal models for human diseases. Fundamental problems in immunology, onset and development of cancer, regulation in fatty acid metabolism, aspects of cardiovascular function, control of the central nervous system development, analysis of reproductive development and function are only some examples of research interests. The final stage of cell differentiation is growth arrest. In animal tissues with rapid cell turnover terminally differentiated cells undergo programmed cell death. The cells have the ability to kill themselves by activating an intrinsic cell suicide program when they are no longer needed or have become seriously damaged. The execution of this program is termed apoptosis. Apoptosis is of importance for development and homeostasis of animals. The key components of this program have been conserved in evolution from worms (C. elegans) to insects (Drosophilia) to humans. The roles of apoptosis include the sculpting of structures during development, deletion of unneeded cells and tissues, regulation of growth and cell number, and the elimination of abnormal and potentially dangerous cells. In this way apoptosis provides "quality control mechanism" that limits the accumulation of harmful cells, such as virus-infected cells and tumor cells. On the other hand inappropriate apoptosis is associated with a wide variety of diseases, including AIDS, neuro-degenerative disorders and ischemic stroke. Because it is now clear that apoptosis is a result of an active, gene-directed process, it should be eventually possible to manipulate this form of cell death by developing drugs that interact with its recently identified mechanisms of action. Inducers of cell differentiation, cell cycle arrest and apoptosis might be the novel molecular targets for new anticancer agents in addition to the signaling pathways for growth factors and cytokines. Proteins, factors, receptors and genes of importance in apoptosis: Proteases: - Calpain, an intracellular cysteine protease, exact role unknown. - Caspase-1 to Caspase-11, a family of proteases synthesized as an inactive proenzyme. Targets of the activated enzymes include: poly(ADP-ribose) polymerase, DNA- dependent protein kinase, Ul ribonucleoprotein, nuclear laminins and cytoskeleton components (actin). - Granzyme B, a serine protease released by cytotoxic T-cells. Receptors: - CD 95 (synonyms: Fas, APO-1), a receptor protein of the TNF -receptor family which includes TNF-R1 and TNF-R2 with the common characteristic of a 70 amino acid cytoplasmic domain. - FADD (synonym: MORT-1), a cytoplasmic protein - DR-3 (synonym: APO-3) a member of the TNF -receptor-family - DR-4 and DR-5 Genes: - ced-3, ced-4 and ced-9 encode the general apoptotic and antiapoptotic program in Caenorhabditis elegans. Apaf-3 is the mammalian homologue of ced-3. - Bcl-2 / Bcl-xL / Bax / Bcl-xS / Bak: a large gene family that can either inhibit or promote apoptosis. - Cytokine response modifier A, a cowpox virus gene whose gene product inhibits caspases. Others: - Caspase-activated DNase (CAD) and its inhibitor (ICAD), causes DNA fragmentation in the nucleus - Ceramide, a complex lipid that acts as a second messenger. - c-Jun N-terminal kinase (JNK) is a proline-directed kinase - p53 protein, is essential for the induction of apoptosis as a response to chromosomal damage. - RAIDD, a death signal-transducing protein. - Receptor interacting protein (RIP) is an accessory protein with a death domain and a serine/threonine kinase activity. - Sphingomyelinase, an enzyme that hydrolyzes the complex lipid sphingomyelin to ceramide. - Tumor necrosis factor (TNF) is a type -II membrane protein - TNF-receptor associated factor (TRAF2), is an accessory protein that can bind to both TNF-R1 and TNF-R2. Within the overall group of Differentiation/Development, several categories of proteins are coded for by clones of the invention: Interleukins (e.g. Interleukin-7): Interleukin precursors related to interleukin-7, for example, are expected to act as new growth factors for human B lineage cells. Additionally, these proteins should induce the gene rearrangement of the T-cell receptor repertoire, leading to thymocyte commitment, and subsequently induce both cytotoxic T-cell- and lymphocyte- activated killer cells These interleukins could find clinical application in a variety of conditions of hematolymphopoietic failure and different tumours, because of its recruitment of B cell lineage cells, cytotoxic T-cell- and lymphocyte-activated killer cells. (OMIN * 146660). Clones in this category include: tes3_35e21. Testis-specific Y-encoded proteins: The TSPY genes are arranged in clusters on the Y chromosome of many mammalian species. TSPY is believed to function in early spermatogenesis and is a candidate for GBY, the putative gonadoblastoma-inducing gene on the Y. Proteins of the TSPY-SET-NAP1L1 family represent proteins closely related to TSPY. These proteins seem to be involved in early spermatogenesis. Clones in this category include: fbr2_2dl5. Intracellular transport and trafficking Eukaryotic cells rely for their viability on the partitioning of many basic cellular processes into membrane-bounded organelles. These are the nucleus, endoplasmic reticulum (ER), Golgi apparatus, endosomes, lysosomal compartments, mitochondria and peroxisomes. Most molecules destined for the lysosome, cell surface and outside the cell are routed through the ER and Golgi, which together with the vesicular intermediates between them, comprise the secretory pathway (Palade 1975). In the ER and Golgi compartments proteins are sorted, modified and often assembled into complexes en route to their final destination. Incorrectly assembled proteins are retained in the ER until they fold correctly or are targeted for degradation. Additional proteins are translocated into and function within the lumenal spaces of organelles or are secreted. Thus a large proportion of proteins synthesized require targeting to membranes either for insertion into or transport across them. A major purpose of this is growth. The secretory pathway is dependent on an intact cytoskeleton and also closely linked to general metabolism by affecting ribosome biogenesis (Mizuta and Warner, 1994). A huge number of proteins is required for targeting, translocation and sorting of newly synthesized proteins. The first step in sorting is the recognition of cis-acting targeting or signal sequences that organelle-targeted proteins contain. This is carried out by cytosolic targeting factors and/or receptors on the membrane to which the protein is targeted. In some cases the primary sequences are extremely degenerate, with only the overall character being conserved (hydrophobicity for an ER signal sequence, helical amphiphilicity for mitochondrial targeting sequence (Kaiser et al, 1987; Lemire et al, 1989). Following the targeting step, proteins are either inserted into or transported across the membrane (translocated) through a proteinaceous apparatus (termed the translocon). The translocon include or recruit motors to drive the translocation process in the correct direction (Schatz and Dobberstein, 1996). Defined intracellular protein transport steps: • ER - targeting to the ER - translocation into the lumen of the ER, and, depending on the presence of certain signals in the peptide sequence transport through the golgi complex • Mitochondria - targeting - translocation • Peroxisomes • The general secretory pathway - protein modification, assembly and quality control in the ER - vesicle-mediated trafficking - vesicle docking and fusion - transport through the golgi apparatus and sorting at the trans-golgi - transport to the cell surface - transport routes to the lysosome • Endocytosis • Specialized protein transport routes • Protein export from the cytoplasm References: Palade, G (1975) Science 189:347-358; Mizuta et al. (1994) Mol Cell Biol 14: 2493-2502; Kaiser et al. (1987) Science 235: 312-317; Lemire et al. (1989) J Biol Chem 264: 20206-20215; Schatz et al. (1996) Science 271 : 1519-1526. Rab proteins In eukaryotic cells the compartmentalisation of processes is a prerequisite for a tight regulation of processes and activities. The cells contain a highly dynamic set of membrane compartments that are responsible for packaging, sorting, secreting, and recycling proteins and other molecules. Trafficking between organelles within the secretory pathway occurs as vesicles derived from a donor compartment fuse with specific acceptor membranes, resulting in the directional transfer of cargo molecules. This process is tightly controlled by the Rab/Ypt family of proteins (reviewed by Novick and Zerial, 1997 ), a branch of the superfamily of small GTPases. Rab proteins regulate a variety of functions, including vesicle translocation and docking at specific fusion sites. Rabs may also play critical roles in higher order processes such as modulating the levels of neurotransmitter release in neurons, a likely mechanism in synaptic plasticity that underlies learning and memory (Geppert and Sudhof, 1998). Small GTPases share a common three-dimensional fold that, in the GTP bound state, can bind a variety of downstream effector proteins. GTP hydrolysis leads to a conformational change in the "switch" regions that renders the GTPase unrecognizable to its effectors. In this way, by localizing and activating a select set of effectors, a common structural motif is used to control a wide array of distinct cellular processes. The final steps in membrane fusion are likely to be driven by a set of proteins known as SNAREs. After a vesicle becomes docked, the cytoplasmic domains of VAMP (also termed synaptobrevin) and syntaxin on opposing membranes, in combination with a SNAP- 25 molecule, coalesce into an elongated -helical bundle (Poirier et al., 1998 ; Sutton et al., 1998 ), which may lead to fusion. Because numerous SNARE isoforms have been identified that localize to distinct membrane compartments, it was originally proposed that the specificity of interaction between the SNARE proteins accounted for the specificity in membrane trafficking. Recent results, however, suggest that SNAREs are not specific in their ability to form complexes in vitro, suggesting that trafficking specificity requires additional factors (Yang et al., 1999 ). In this regard, Rab proteins are strong candidates for governing the specificity of vesicle trafficking. Like the SNAREs, many isoforms (40) of the Rab family have been identified that localize to specific membrane compartments (reviewed by Novick and Zerial, 1997 ). Concomitant with the SNARE cycle, Rab proteins undergo a intricate cycle of membrane and protein interactions. Rabs are posttranslationally modified at C-terminal cysteines by the addition of two geranylgeranyl groups, which mediate membrane association when the Rab is in the GTP-bound state. After guanine nucleotide hydrolysis occurs, the Rab is extracted from the membrane upon forming a complex with a cytosolic GDP-dissociation inhibitor (GDI). This cytosolic intermediate is then recycled onto a newly forming vesicle, most likely through a secondary factor termed a GDI dissociation factor (GDF), which displaces GDI. After the Rab becomes membrane bound, a guanidine nucleotide exchange factor (GEF) promotes release of GDP and the subsequent loading of GTP. In its GTP-bound conformation, the Rab is then free to associate with its specific set of effectors, which can in turn trigger events leading to the eventual fusion of the vesicle with a target membrane. To complete the cycle, perhaps after or concurrent with membrane fusion, a GTPase activating protein (GAP) accelerates nucleotide hydrolysis, switching off the GTPase. The remaining GDP-bound Rab can then participate in a new round of fusion. Rab interactions with effectors are likely to regulate vesicle targeting and membrane fusion in three ways. First, a Rab may specifically facilitate vectorial vesicle transport. Vesicles are transported from their site of origin to acceptor compartments likely through associations with cytoskeletal elements and transport motors. A protein has been identified with a domain structure that suggests a connection between the cytoskeleton and the Rabs. This protein, called Rabkinesin-6, contains a kinesin-like ATPase motor domain followed by a coiled-coil stalk region and a RBD that specifically binds Rab6 (Echard et al., 1998 ). An additional link with the cytoskeleton is provided by the Rab effector, Rabphilin-3A. Rabphilin-3A has been shown in vitro to interact with -actinin, an actin-bundling protein, but only when not bound to Rab3A (Kato et al., 1996 ). These results raise the intriguing possibility that Rab proteins regulate vesicle interactions with the cytoskeleton and thereby play an active role in targeting vesicles to their appropriate destinations. Second, Rab proteins may regulate membrane trafficking at the vesicle docking step. A number of Rab effectors, including Rabaptin-5, EEA1, Rabphilin-3A, and Rim, may serve as molecular tethers. Each effector protein contains a RBD, followed by a linker region (some having the potential to form elongated coiled-coil structures), and a domain capable of interacting with a second Rab or the target membrane. Rabaptin-5, for example, contains two RBDs, one near the N terminus that specifically recognizes Rab4 and a second near the C terminus that binds Rab5 (Vitale et al., 1998 ). Both Rim, which is localized to the target membrane, and Rabphilin-3 A, which is localized to the vesicle, contain N-terminal RBDs and C-terminal Ca2+-binding C2 domains, implicating these effectors in synaptic vesicle localization or docking in response to Ca2+ influx (Wang et al., 1997 ). Tethering effectors may also recognize protein complexes on the acceptor membrane. Sec4p, a yeast Rab3A homolog, interacts with the exocyst (Guo et al., 1999 ), a complex of seven or more subunits that is assembled at sites of vesicle fusion along the plasma membrane. The exocyst complex may therefore function as a landmark for Rab/effector-mediated vesicle docking. Third, once a vesicle has become tethered to its fusion site, Rab proteins may selectively activate the SNARE fusion machinery. The mechanism of this activation is unknown but may involve direct interactions of Rabs or, more likely, their effectors with SNAREs. For example, Hrs-2 is a protein that binds to SNAP-25 and contains a Zn2+-fϊnger motif characteristic of Rab-binding proteins such as Rabphilin-3 A, Rim, EEA1, and Noc2, suggesting that Hrs-2 may form a physical link between Rabs and SNAREs (Bean et al., 1997). In addition, certain mutations in the syntaxin-binding protein Slylp, the Seclp homolog utilized in ER to Golgi trafficking, eliminate the requirement for Yptlp, a Rab protein that functions at this trafficking step (Dascher et al., 1991 ). Rabs may therefore regulate SNARE associations through Seel family members. In support of this idea, a Rab effector was recently found to interact with a vacuole Rab, a Seclp homolog, and a SNARE protein (Peterson et al., 1999 ), which suggests that this effector serves to connect Rab and SNARE function. In this way, Rabs and their effectors may facilitate the correct pairing of SNAREs. References: Dascher et al. (1991) Mol. Cell. Biol. 11, 872-885; Echard et al. (1998). Science. 279, 580-585; Geppert et al. (1998) Annu. Rev. Neurosci. 21, 75-95; Guo et al. (1999). EMBO J. 18, 1071-1080; Kato et al. (1996) J. Biol. Chem. 271, 31775-31778; Novick et al. (1997) Curr. Opin. Cell Biol. 9, 496-504; Peterson (1999) Curr. Biol. 9, 159- 162; Poirier et al. (1998) Nat. Struct. Biol. 5, 765-769; Vitale et al. (1998) EMBO J. 17, 1941-1951; Wang et al. (1997) Nature. 388, 593-598; Yang et al. (1999) J. Biol. Chem. 274, 5649-5653. Within the overall group of Intracellular Transport and Trafficking several categories of proteins are coded for by clones of the invention. Rab proteins: Rab IB is essential for the intracellular transport of nascent low density lipoprotein (LDL) receptor. It is discussed as a universal mediator of endoplasmatic reticulum to Golgi transport of membrane glycoproteins in mammalian cells. . Clones in this category include: fbr2 2il7, fbr2 3b 16. Rab 10 appear concentrated on membranes in the perinuclear region. Rab 10 has been associated (as potentially diagnostic, therapeutic, causative, and or related, etc..) with the following diseases as reported by OMIN: 1) Choroideremia (OMIN *303199); and 2)RETT Syndrome (OMIN 312750). Clones in this category include: fbr2_62119. In mice, Rab 17 shows epithelial cell specificity. Rab 17 is discussed as candidate gene for the mouse mutations In (leaden), Tw (twirler), and ax (ataxia). Cloned from a brain cDNA library, the new putative Rab-protein is expected to be involved in vesicle trafficking within neuronal cells. These proteins can find application in modulating the transport of vesicles inside neuronal cells, which are essential for development of functional dendritic processes. . . Clones in this category include: fbr2_41ml5. Ankyrin G: The ankyrin 3 gene encodes a novel ankyrin, which is expressed in multiple tissues, with very high expression at the axonal initial segment and nodes of Ranvier of neurons in the central and peripheral nervous systems. Ankyrin G shows several tissue- specific alternative mRNA processing. The different ankyrin G proteins participate in maintenance/targeting of ion channels and cell adhesion molecules to nodes of Ranvier and axonal initial segments. Ankyrin G has been associated (as potentially diagnostic, therapeutic, causative, and/or related, etc ..) with Werner disease (OMIN *277700). Clones in this category include: fkd2_24p5. Zn-T-transporters : The Zn-T-transporters are membrane proteins that facilitates sequestration of zinc in endosomal vesicles. In the brain, ZnT-3 mRNA seems to be involved in the accumulation of zinc in synaptic vesicles. Zinc (Zn) is an essential element in normal development and metabolism. Recent studies show that in Alzheimer's disease, Zn functions as a double-edged sword, affording protection against Alzheimer's amyloid beta peptide (the major component of senile plaques) at low concentrations and enhancing toxicity at high concentrations by accelerated aggregation of the amyloid beta peptide. These proteins can find application in modulation of Zinc transport in neuronal cells, thus providing means for a modulation of Alzheimer's amyloid beta peptide plaque formation. (OMIN *602878, *602095). Clones in this category include: fbr2_62fl0. Metabolism This group includes proteins which are involved in the uptake and consumption of nutrients, and enzymes which are part of the biochemical pathways for energy metabolism or which are involved in the supply of building blocks of nucleic acids, proteins (NTPs, dNTPs, amino acids) for DNA/RNA and protein synthesis, and fatty acids (membranes), to allow for the generation of higher order structures. This group constitutes the most important and largest group in prokaryotes and lower eukaryotes. The higher the evolutionary level of an organism is, however, the more other protein classes like 'signal transduction', 'cell cycle' and 'differentiation and development' increase in importance and number of representatives. Proteins involved in the metabolism of energy and compounds (here: other than nucleic acids or proteins) are usually the products of house keeping genes, they are often constitutively and/or ubiquitously expressed. Several categories of proteins are coded for by clones of the invention within the overall group of Metabolism: NAT1. ARD1 : In yeast, ARD1 and NAT1, are required for the expression of an N- terminal protein acetyltransferase 1. NAT1 controls full repression of the silent mating type locus HML, sporulation and entry into GO. ARD1 is involved in the assembly of the NAT 1- complex. These can find application modulating NAT assembly and action and therefore could be important in metabolism of drugs and environmental mutagens.(OMIN * 108345). Clones in this category include: fbr2_3g8. Apolipoprotein E receptor: In LDL-receptors the class A domains form the binding site for LDL and calcium. The acidic residues between the fourth and sixth cysteines are important for high-affinity binding of positively charged sequences in LDLR's ligands. These proteins can find application in modulation of cholesterol binding and transport by LDL- receptors and LDL-binding proteins. In normal individuals, chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. In familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants because of a defect in apolipoprotein E. Accumulation of the remnants can result in xanthomatosis and premature coronary and or peripheral vascular disease. OMIN reports that apolipoprotein has associations (as potentially diagnostic, therapeutic, causative, and/or related, etc..) with the following diseases: 1) Familial hypercholesterolemia (OMIN 143890); 2) Familial combined hyperiipidemia (OMIN 144250); and 3) Alzheimer disease. (OMIN #104300). Clones in this category include: fbr2_62017. Ubiquitin carboxyl-terminal hydrolases: Ubiquitin carboxyl-terminal hydrolases (EC 3.1.2.15) (UCH) (deubiquitinating enzymes) are thiol proteases that recognize and hydrolyze the peptide bond at the C-terminal glycine of ubiquitin. These enzymes are involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins. OMIN reports that Ubiquitin-specific proteases have associations (as potentially diagnostic, therapeutic, causative, and or related, etc..) with the following diseases: 1) Lung carcinoma (OMIN *603486); 2) x-linked retinal diseases (OMIN *300050); 3) oncogenesis (OMIN *300050);4) ovarian cancer (OMIN *300050). Clones in this category include: fbr2_78k24; htes3_27dl. Phosphoserine signature (phosphoglucomutases. phosphomannomutase): These proteins take part in the conversion of hexose phosphates. OMIN reports that these proteins have associations (as potentially diagnostic, therapeutic, causative, and/or related, etc...) with the following disease: Fanconi-Bickel Syndrome (OMIN #227810). Clones in this category include: fkd2_24bl5. NADH ubiquinone oxidoreductase: NADH:ubiquinone oxidoreductase is the first enzyme in the respiratory electron transport chain of mitochondria. It is a a membrane-bound multi-subunit protein. The bovine heart enzyme contains about 40 different polypeptides. OMIN reports that these proteins have associations (as potentially diagnostic, therapeutic, causative, and/or related, etc ..) with the following disease: Brancio-oto-renal syndrome (OMIN *6601445). Clones in this category include: fkd2_3ol7. Transketolases: Transketolase requires thiamin pyrophosphate as cofactor and shows a wide specificity for both reactants, e.g. converts hydroxypyruvate and R-CHO into CO(2) and R-CHOH-CO-CH(2)OH. OMIN reports that these proteins have associations (as potentially diagnostic, therapeutic, causative, and or related, etc..) with the following diseases: Wernicke-Korsakoff Syndrome (OMIN *277730). Clones in this category include: tes3_17117. Fatty acid-Co A svnthetases/li gases: These proteins contain AMP-binding domain signature(s), which is present in enzymes which act via an ATP-dependent covalent binding of AMP to their substrate. This domain is found in several CoA synthetases, such as acetate- CoA ligase (EC 6.2.1.1), long-chain-fatty-acid-CoA ligase (EC 6.2.1.3), bile acid-CoA ligase. OMIN reports that these proteins have associations (as potentially diagnostic, therapeutic, causative, and or related, etc..) with the following diseases: 1) Alport syndrome , mental retardation and elliptocytosis (OMIN *300157); 2) Adrenoleukodystrophy (OMIN *300100). Clones in this category include: tes3_35kl7. ADP/ATP or Adenine Nucleotide Translocataors: These proteins contain mitochondrial energy transfer signature(s) and are most abundant in mitochondria. In its functional state, it is a homodimer of 30-kD subunits embedded asymmetrically in the inner mitochondrial membrane. The dimer forms a gated pore through which ADP is moved from the matrix into the cytoplasm.. OMIN reports that these proteins have associations (as potentially diagnostic, therapeutic, causative, and/or related, etc...) with the following diseases: 1) cardiomyopathy (OMIN * 103220); 2) myopathy (OMIN * 103220); 3)Progressive external ophthalmoplegia (OMIN *601227). Clones in this category include: tes3_35nl2. Carboxylesterases : OMIN reports that these proteins have associations (as potentially diagnostic, therapeutic, causative, and or related, etc...) with the following diseases: l)hepatic carboxylesterase with detoxification of foreign compounds (OMIN *114835); 2) non-Hodgkin lymphoma (OMIN *114835); 3) B-cell chronic lymphocytic leukemia (OMIN * 114835); 4) rheumatoid arthritis (OMIN * 114835). Clones in this category include: tes3_35n9. Heat shock proteins: OMIN reports that these proteins have associations (as potentially diagnostic, therapeutic, causative, and or related, etc ..) with the following diseases: 1)27 kd heat shock protein has been correlated with thermotolerance in response to environmental challenges and developmental transitions. (OMIN *6021295). Clones in this category include: utell_23el3. Nucleic acid management The genetic information is stored in the form of nucleic acids in all organisms. Two kinds of nucleic acids exist, DNA and RNA. Whereas the more stable DNA in most organisms constitutes the storage form of the genetic information, the labile RNA and in particular mRNA is an intermediate used for the temporal expression of specific genes. In eukaryotes, DNA is usually a double stranded linear molecule consisting of two antiparallel strands and made up of a deoxyribose, a phosphorus backbone and the four bases A, C, G, and T. The DNA of some organisms has a ring structure. The structure of DNA was unraveled years ago by Watson and Crick. DNA is directional molecule determined by the C- atoms of the sugar. The most important processes dealing with nucleic acids are: • replication (e.g. DNA polymerases, Telomerase) • transcription (RNA polymerases) • RNA processing (maturation - splicing and degradation) • in addition, enzymes and proteins exist which require a nucleic acid (mostly RNA) in the active center to be functional (ribozymes - e.g. RNase, Ribosomal proteins) The DNA of a cell is replicated in the S-phase of the cell cycle. Several enzymes carry out the task of doubling this nucleic acid. As all steps of the cell cycle, also the process of replication is tightly regulated. The enzyme DNA polymerase and several other proteins are involved in this process. Whereas many prokaryotes do have only one origin of replication (i.e., the starting point of the replication cycle), in eukaryotic DNAs (chromosomes) multiple such start points exist. The switch from the synthesis (S) phase to the subsequent G2 or M phases of the cell cycle are dependent on the completion of the replication. This makes clear, that a number of proteins are involved in the replication itself as well as in the control of the process. Since most eukaryotic chromosomes are linear structures, additional proteins and enzymes are necessary to make sure that the structure is maintained through successive generations. This includes those proteins necessary to build the three dimensional structure of chromosomes (e.g. histones) and the structural network of the nucleus and nucleolus (including the defined localization of transcriptionally active genes in the vicinity of nucleoli) but also such enzymes as telomerase which guarantees the integrity of the chromosomal ends. The expression of genes is usually performed in two steps. First a messenger RNA (mRNA) is produced (transcribed) in one to many copies and second this mRNA is translated into the protein product. The regulation of transcription is discussed under the separate heading 'transcription factors', but also the classes 'signal transduction', 'development', 'cell cycle' and others are affected as the expression of certain genes determines the fate of a cell or organism. The primary transcript (hnRNA - heterogeneous nuclear RNA) is a single stranded one-to-one copy of the gene as it is located on the chromosome. Before a protein can be translated, already during transcription the process of maturation is initiated. Firstly, a 5' cap structure is enzymatically and covalently added to the RNA, blocking the 5' end of the RNA. Second, when the RNA polymerase has terminated polymerization, the enzyme poly A polymerase adds varying numbers of adenine residues to the 3' end of the transcript. This enzyme recognizes the sequence AAUAAA or AUUAAA (+ some minor variations), cuts the RNA 10 - 30 nucleotides downstream and adds the A residues. The size of the poly A sequence affects the stability of the RNA. Finally, in the process of splicing, the introns present on the genomic level and also present in the hnRNA are spliced out by a multi-protein complex consisting of several proteins and RNAs. The finally maturated mRNA is exported to the cytoplasm where it is translated with help of the ribozymes. The half life of RNA is usually much shorter than that of DNA. Usually, the mRNA is degraded shortly after synthesis, to guarantee a very defined window of expression of a given gene. This regulation is necessary to specifically maintain or change the set of proteins present at any time in a cell. Specific regions in the 3'UTR (untranslated region) determine the stability of the mRNA in the cytoplasm before it is degraded by RNases, enzymes consisting both of protein and RNA. References: Watson and Crick (1953) Nature 171: 737-738. Several categories of proteins are coded for by clones of the invention within the overall group of "Nucleic acid managemenf'and include, among others, the following: RNA helicases including DEAD/H box helicases: RNA helicases comprise a large family of proteins that are involved in basic biological systems such as nuclear and mitochondrial splicing processes, RNA editing, rRNA processing, translation initiation, nuclear mRNA export, and mRNA degradation. RNA helicases are essential factors in cell development and differentiation, and some of them play a role in transcription and replication of viral single-stranded RNA genomes. The members of the largest subgroup, the DEAD and DEAH box proteins, exhibit a strong dependence of the unwinding activity on ATP hydrolysis. DEAD box proteins have been associated (as potentially diagnostic, therapeutic, causative, and/or related, etc..) as reported by with the following disease processes and/or genes: 1) ataxia-telangiectasia gene: "A human gene (DDX10) encoding a putative DEAD- box RNA helicase at 1 Iq22-q23" Genomics 33:199-206, 1996, Savitsky et al., (OMIN *601235); 2) hematopoetic tumors: "Cloning and expression of a murine cDNA homologous to the human RCK P54, a lymphoma-linked chromosomal breakpoint 1 lq23", Gene 166:293- 6, 1995, Seto et al. (OMIN *600326); 3) dermatomyositis: a) "The major dermatomyositis- specific Mi-2 autoantigen is a presumed helicase involved in transcriptional activation." Arthritis Rheum. 38: 1389-1399, 1995, Seelig et al. (OMIN *603277); b) "Two forms of the major antigenic protein of the dermatomyositis-specific Mi-2 autoantigen." (Letter), Arthritis Rheum. 39: 1769-1771, 1996., Seelig et al. (OMIN *603277); c) "The dermatomyositis- specific autoantigen Mi2 is a component of a complex containing histone deacetylase and nucleosome remodeling activities", Cell 95: 279-289, 1998. Zhang et al. (OMIN *603277); 4) Muscular Dystrophy, Pseudohypertrophic Progressive Duchenne and Becker Types (OMIN *310200); 5) Mucopolysaccharidosis Type IVA (OMIN *253000); 6) Albinism I (OMIN *203100); 7) Wilms Tumor 1 (OMIN * 194070); 8) Spinocerebellar Ataxia 7 (OMIN * 164500). Clones in this category include: fbr2_23bl0, fbr2_3cl8, fbr2_6ol7, fbr2_82i24, and tes3_14h21. Inorganic pyrophosphatase: Inorganic pyrophosphatase (EC 3.6.1.1) (PPase) is the enzyme responsible for the hydrolysis of pyrophosphate (PPi) which is formed as the product of the many biosynthetic reactions that utilize ATP. All known PPases require the presence of divalent metal cations, with magnesium conferring the highest activity. Clones in this category include: fbr2_64al5. DNA-damage -inducible protein (dinP) or Proteins induced by DNA-Damage: The dinB/P pathway is a second SOS-pathway in E.coli. Genes related to this seem to be involved in modulating DNA repair and mutagenesis. Clones in this category include: fbr2_72M8 i Proteins with myc-tvpe, helix-loop-helix dimerization domain signature(s). This helix-loop-helix domain mediates protein dimerization has been found in proteins such as the myc family of cellular oncogenes, proteins involved in myogenesis and vertebrate proteins that bind specific DNA sequences in various immunoglobulin chains enhancers. Therefore, these proteins could be novel DNA-binding proteins. Clones in this category include: fbr2_72112. Cytosolic ribosomal proteins L36: L36 seems to be part of the eukaryotic ribosomal peptidyl transferase center and can find application in modulation of ribosome assembly, maintenance and activity. Clones in this category include: fkd2_3b2. Ribonuclease H: Ribonuclease H proteins are RNA modificating proteins and have been associated (as potentially diagnostic, therapeutic, causative, and/or related, etc...) with the following diseases as reported by OMIN: 1) Adenomatous Polyposis of the Colon (OMIN * 175100); 2) Retinoblastoma (OMIN * 180200) ; and 3) Von Hippel-Lindau Syndrome (OMIN * 193300). Clones in this category include: phtes3_15j3. Signal transduction Cells in higher order organisms need to continuously communicate with its environment especially with other cells of the same organism in order to maintain the function and specialization of the whole system these cells are part of. This important task of communication is performed with help of cell-surface receptors which receive and transmit signals from outside into the cell. G-proteins The largest known family of cell-surface receptors is that of the G-protein-coupled receptors, which mediate the transmission of diverse stimuli such as neurotransmitters, glycopeptides, hormones, peptides, odorant molecules, and photons. The functional unit of these receptors is composed of the receptor molecule itself (GPCR) which is anchored in the cytoplasma membrane with seven membrane spanning domains, the heterotrimeric G-protein which is composed of α and βγ-subunits (Gα and Gβγ), and the effectors that interact with Gα and / or Gβγ. In particular, the dissociated Gα and Gβγ can regulate the activities of a number of effector molecules such as adenylate cyclases, phopholipase C isoforms, ion channels, and tyrosine kinases, resulting in a variety of cellular functions. The process of signal transduction must be tightly regulated and reversible in order to avoid overstimulation, to achieve signal termination, and render the receptor responsive to subsequent stimuli [Iacovelly L. et al., (1999) FASEB J. 13, 1-8, Hamm, H.E. (1998) J. Biol. Chem. 273, 669- 672]. G-proteins are GTPases that, upon binding of GTP change their conformation which in return unmasks structural motives, in particular the so called effector loop, which can mediate the interactions to target proteins, or effectors, for the GTPases. This ability enables the GTPases to cycle between active, GTP-bound and inactive, GDP bound conformations and in the process to function as molecular traffic lights in a multitude of signal transduction pathways. The most important of these signal transduction pathways that are regulated with help of G-proteins are that of the phospholipase C / protein kinase C and that of the adenylate cyclase / protein kinase A. The cycling of GTPases is tightly regulated by three main classes of proteins: The exchange of hydrolyzed GDP for a fresh GTP is facilitated by guanosine nucleotide exchange factors (GEFs), the hydrolysis of GTP to GDP is sped up by GTPase-activating proteins (GAPs), and the dissociation of GDP from the GTPases is inhibited by GDP dissociation inhibitors (GDIs) [Tapon and Hall (1997) Curr.Opin. Cell. Biol. 9, 86-92, Van Aelst and D- Souza-Schorey (1997) Genes Dev. 11, 2295-2322]. SOC-familv A conserved motif that was originally identified in proteins that negatively regulate the signaling action of cytokines was termed SOCS box, the Suppressor Of Cytokine Signaling. Based on homology, five distinct structural protein classes have been identified since that carry this motif. The function of most of these proteins is presently not known. Common to the proteins is only the SOCS box which is located near the C-terminus of the respective peptides. Recently, the SOCS box has been demonstrated to induce binding of proteins to elongins B and C which could target the proteins (and bound substrates) to the proteasomal protein degradation pathway (Kamura, T. et al. (1998) Genes Dev. 12, 3872- 3881; Zhang, J.-G. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 2071-2076). The class where the SOCS box was originally described contains several members (SOCS-l-SOCS-7 and CIS). In addition to the SOCS box, these proteins also contain a SH2 (Src-homology 2) domain and a variable N-terminus. These SOCS proteins appear to form part of a classical negative feedback loop that regulates cytokine signal transduction. Upon cytokine stimulation, expression of SOCS proteins is rapidly induced and the proteins inhibit further cytokine action. The mode of action of the SOCS proteins is variable. While SOCS-1 binds and inhibits the JAK (Janus kinases) family of cytoplasmic protein kinases [Narahzaki M. et al (1998) Proc. Natl. Acad. Sci. USA 95, 13130-13134, Nicholson, S.E. et al (1999) EMBO. J. 18, 375-385], CIS appears to act by competing with signaling molecules such as the STATs (Transducers and Activators of Transcription) family for binding to phosphorylated receptor cytoplasmic domains [Yoshimura, A. et al. (1995) EMBO J. 14, 2816-2826; Matsumoto, A. et al (1997) BloodS9, 3148-3154]. A second class of SOCS box protein contains additionally WD-40 repeats which were initially identified in the mouse WSB-1 and -2 proteins. The functions of WD-40 proteins are not completely understood but seem to be rather divergent. In Cdc4p the WD-40 repeats probably are necessary for binding the substrate for Cdc34p [Mathias, N. et al. (1999) Mol. Cell Biol. 19, 1759-1767]. Cdc4p is a component of a ubiquitin ligase that tethers the ubiquitin-conjugating enzyme Cdc34p to its substrates. The posttranslational modification of a protein by ubiquitin usually results in rapid degradation of the ubiquitinated protein by the proteasome. The transfer of ubiquitin to substrate is a multistep process where WD-40 repeats might play an important function. Other WD-40 containing proteins (e.g. the retino blastoma binding protein RbAp48) have been shown to bind metal ions (Zinc) and that this metal binding might mediate and/or regulate protein-protein interactions which are functionally important in chromatin metabolism [Kenzior, A.L. and Folk, W.R. (1998) FEBSLett. 440, 425-429]. These proteins are involved in the RAS-cAMP pathway that regulates cellular growth [Ach R.A. et al. (1997) Plant Cell 9, 1595-1606]. The SPRY domain has been identified in pyrin or marenostrin, a protein which is mutated in patients with Mediterranean fever and which is similar to the butyrophilin family. While butyrophilins seem to be involved in the lactation process in mammals, the function pyrin is unknown. Three proteins (SSB-1 to -3) have been identified to contain both SPRY and SOCS box motifs. The function of these proteins is also not known. Ankyrin repeat containing proteins share a 33-residue repeating motif, an L-shaped structure with protruding β-hairpin tips which mediate specific macromolecular interactions with cytoskeletal, membrane, and regulatory proteins. These proteins play fundamental roles in diverse biological activities including growth and development, intracellular protein trafficking, the establishment and maintenance of cellular polarity, cell adhesion signal transduction, and mRNA transcription. Three proteins that contain ankyrin repeats (ASB-1 to -3) have been identified to contain a C-terminal SOCS box additionally to the ankyrin repeats. The function of these proteins or the individual domains remains to be discovered [Hilton, D.J. et al (1998) Proc. Natl. Acad. Sci. USA 95, 114-119]. A few small GTPases (RAR and RAR like) do also contain a SOCS box. GTPases are involved in signal transduction during cellular communication. The function of the SOCS box in this type of proteins is currently unclear [Hilton, D.J. et al (1998) Proc. Natl. Acad. Sci. USA 95, 114-119]. Ca 2+ as second messenger The bivalent cation Ca 2+ is, besides cAMP, one of the two major second messengers in eukaryotic cells. Its intracellular concentration is tightly regulated and usually kept very low compared to the cell's environment. Ca 2+ binding proteins and transporters (Gap junction, Voltage-gated, second messenger-gated) help to sequester huge amounts of the ion in various organelles from where Ca 2+ can be released upon extracellular stimuli. E.g. the contraction of the muscle is dependent on the presence of Ca 2+ ions which are readily transported back into the organelles in order for the muscle to relax. In signal transduction, Ca 2+ functions as a second messenger that activates Ca 2+ dependent processes through the activation of Ca 2 7calmodulin dependent protein kinases (CaM kinases) which are the major effector molecules of Ca 2+ . In the signaling cascades, the CaM dependent kinases activate phospholipases (e.g. phospholipase C) that in return activate other protein kinases such as protein kinase C. cAMP The cyclic AMP is produced by the enzyme adenylate cyclase in response to extracellular signals. Certain G-proteins stimulate the activity of adenylate cyclase which converts ATP to cAMP and PPi. Two molecules of cAMP bind to each of two regulatory subunits of cAMP dependent protein kinase which in turn dissociate from the two catalytic subunits of the heterotetramer R 2 C 2 . Upon release of the C-subunits, they become active and phosphorylate substrate proteins at Ser and Thr residues. The process leading from binding of extracellular molecules to their receptors, the transmission of the stimuli into the cell, the activation of adenylate cyclase and the subsequent activation of cAMP dependent protein kinase is one of two major signal transduction pathways in eukaryotic cells. Since the phosphorylation of proteins is a posttranslational modification of proteins, the kinases are described in the class "signal transduction." SARA Members of the transforming growth factor β (TGFβ) superfamily signal through a family of cell-surface transmembrane serine/threonine kinases, known as type I and type II receptors (Heldin et al., 1997 ; Attisano and Wrana, 1998 ; Kretzschmar and Massague, 1998). Ligand induces formation of heteromeric complexes of these receptors, and signaling is initiated when receptor I is phosphorylated and activated by the constitutively active kinase of receptor II (Wrana et al., 1994 ). The activated type I receptor kinase then propagates the signal to a family of intracellular signaling mediators known as Smads (contraction of the C.elegans Sma and Drosophila Mad genes which were the first identified members of this class of signaling effectors). Three classes of Smads with distinct functions have been defined: the receptor- regulated Smads, which include Smadl, 2, 3, 5, and 8; the common mediator Smad, Smad4; and the antagonistic Smads, which include Smad6 and 7 (Heldin et al., 1997; Attisano and Wrana, 1998 ; Kretzschmar and Massague, 1998 ). Receptor-regulated Smads (R-Smads) act as direct substrates of specific type I receptors, and the proteins are phosphorylated on the last two serines at the carboxyl terminus within a highly conserved SSXS motif (Macias-Silva et al., 1996 ; Abdollah et al., 1997 ; Kretzschmar et al., 1997 ; Liu et al., 1997b ; Souchelnytskyi et al., 1997 ). Regulation of R-Smads by the receptor kinase provides an important level of specificity in this system. Thus, Smad2 and Smad3 are substrates of TGFβ or activin receptors and mediate signaling by these ligands (Macias-Silva et al., 1996 ; Liu et al., 1997b ; Nakao et al., 1997 ), whereas Smadl, 5, and 8 are targets of BMP receptors and propagate BMP signals (Hoodless et al., 1996 ; Chen et al., 1997b ; Kretzschmar et al., 1997 ; Nishimura et al., 1998 ). Once phosphorylated, R-Smads associate with the common Smad, Smad4 (Lagna et al., 1996 ; Zhang et al., 1997 ), and mediate nuclear translocation of the heteromeric complex. In the nucleus, Smad complexes then activate specific genes through cooperative interactions with DNA and other DNA-binding proteins such as FASTI, FAST2, and Fos/Jun (Chen et al., 1996 , Chen et al., 1997a ; Liu et al., 1997a ; Labbe et al., 1998 ; Zhang et al., 1998 ; Zhou et al., 1998 ). In contrast to R-Smads and Smad4, the antagonistic Smads, Smadό and 7, appear to function by blocking ligand-dependent signaling (reviewed in Heldin et al., 1997 ). Phosphorylation of R-Smads by the type I receptor is essential for activating the TGFβ signaling pathway (Heldin et al., 1997 ; Attisano and Wrana, 1998 ; Kretzschmar and Massague, 1998 ). However, little is known of how Smad interaction with receptors is controlled. A novel Smad2/Smad3 interacting protein has been described (Tsukazaki T. et al., 1998 ) that contains a double zinc finger, or FYVE domain, and which has been called SARA (Smad anchor for receptor activation). The SARA motif recruits Smad2 into distinct subcellular domains and co-localizes and interacts with TGFβ receptors. TGFβ signaling induces dissociation of Smad2 from SARA with concomitant formation of Smad2/Smad4 complexes and nuclear translocation. Moreover, deletion of the FYVE domain in SARA causes mislocalization of Smad2 and inhibits TGFβ-dependent transcriptional responses. Thus, SARA defines a component of TGFβ signaling that functions to recruit Smad2 to the receptor by controlling the subcellular localization of Smad. References: Abdollah et al. (1997) J. Biol. Chem. 272, 27678-27685; Attisano et al. (1998) Curr. Opin. Cell Biol. 10, 188-194; Chen et al. (1996) Nature 383, 691-696; Chen et al. (1997a) Nature 389, 85-89; Chen et al. (1997b) Proc. Natl. Acad. Sci. USA 94, 12938-12943; Heldin et al. (1997) Nature 390, 465-471; Hoodless et al. (1996) Cell 85, 489-500; Kretzschmar et al. (1998) Curr. Opin. Genet. Dev. 8, 103-111; Kretzschmar et al. (1997) Genes Dev. 11, 984-995; Labbe et al. (1998) Mol. Cell 2, 109-120; Lagna et al. (1996) Nature 383, 832-836; Liu et al. (1997a) Genes Dev. 11, 3157-3167; Liu et al. (1997b) Proc. Natl. Acad. Sci. USA 94, 10669-10764; Macias-Silva et al. (1996) Cell 87, 1215-1224; Nakao et al. (1997) EMBO J. 16, 5353-5362; Nishimura et al. (1998) J. Biol. Chem. 273, 1872-1879; Souchelnytskyi et al. (1997) J. Biol. Chem. 272, 28107-28115; Tsukazaki et al. (1998) Cell 95, 779-791; Wrana et al. (1994) Nature 370, 341-347; Zhang et al. (1997) Curr. Biol. 7, 270-276; Zhang et al. (1998) Nature 394, 909-913; Zhou et al. (1998) Mol. Cell 2, 121-127. Calcium The bivalent cation Ca 2+ is, along with cAMP, one of the two major second messengers in eukaryotic cells. Its intracellular concentration is tightly regulated and usually kept very low compared to the cell's environment. Ca 2+ binding proteins and transporters (Gap junction, Voltage-gated, second messenger-gated) help to sequester huge amounts of the ion in various organelles from where Ca 2+ can be released upon extracellular stimuli. E.g. the contraction of the muscle is dependent on the presence of Ca 2+ ions which are readily transported back into the organelles in order for the muscle to relax. In signal transduction, Ca 2+ functions as a second messenger that activates Ca 2+ dependent processes through the activation of Ca 2 7calmodulin dependent protein kinases (CaM kinases) which are the major effector molecules of Ca 2+ . In the signaling cascades, the CaM dependent kinases activate phospholipases (e.g. phospholipase C) that in return activate other protein kinases such as protein kinase C. Rab proteins In eukaryotic cells the compartmentalization of processes is a prerequisite for a tight regulation of processes and activities. The cells contain a highly dynamic set of membrane compartments that are responsible for packaging, sorting, secreting, and recycling proteins and other molecules. Trafficking between organelles within the secretory pathway occurs as vesicles derived from a donor compartment fuse with specific acceptor membranes, resulting in the directional transfer of cargo molecules. This process is tightly controlled by the Rab/Ypt family of proteins (reviewed by Novick and Zerial, 1997 ), a branch of the superfamily of small GTPases. Rab proteins regulate a variety of functions, including vesicle translocation and docking at specific fusion sites. Rabs may also play critical roles in higher order processes such as modulating the levels of neurotransmitter release in neurons, a likely mechanism in synaptic plasticity that underlies learning and memory (Geppert and Sudhof, 1998 ). Small GTPases share a common three-dimensional fold that, in the GTP bound state, can bind a variety of downstream effector proteins. GTP hydrolysis leads to a conformational change in the "switch" regions that renders the GTPase unrecognizable to its effectors. In this way, by localizing and activating a select set of effectors, a common structural motif is used to control a wide array of distinct cellular processes. The final steps in membrane fusion are likely to be driven by a set of proteins known as SNAREs. After a vesicle becomes docked, the cytoplasmic domains of VAMP (also termed synaptobrevin) and syntaxin on opposing membranes, in combination with a SNAP- 25 molecule, coalesce into an elongated -helical bundle (Poirier et al., 1998 ; Sutton et al., 1998 ), which may lead to fusion. Because numerous SNARE isoforms have been identified that localize to distinct membrane compartments, it was originally proposed that the specificity of interaction between the SNARE proteins accounted for the specificity in membrane trafficking. Recent results, however, suggest that SNAREs are not specific in their ability to form complexes in vitro, suggesting that trafficking specificity requires additional factors (Yang et al., 1999 ). In this regard, Rab proteins are strong candidates for governing the specificity of vesicle trafficking. Like the SNAREs, many isoforms (40) of the Rab family have been identified that localize to specific membrane compartments (reviewed by Novick and Zerial, 1997 ). Concomitant with the SNARE cycle, Rab proteins undergo a intricate cycle of membrane and protein interactions. Rabs are posttranslationally modified at C-terminal cysteines by the addition of two geranylgeranyl groups, which mediate membrane association when the Rab is in the GTP-bound state. After guanine nucleotide hydrolysis occurs, the Rab is extracted from the membrane upon forming a complex with a cytosolic GDP-dissociation inhibitor (GDI). This cytosolic intermediate is then recycled onto a newly forming vesicle, most likely through a secondary factor termed a GDI dissociation factor (GDF), which displaces GDI. After the Rab becomes membrane bound, a guanidine nucleotide exchange factor (GEF) promotes release of GDP and the subsequent loading of GTP. In its GTP-bound conformation, the Rab is then free to associate with its specific set of effectors, which can in turn trigger events leading to the eventual fusion of the vesicle with a target membrane. To complete the cycle, perhaps after or concurrent with membrane fusion, a GTPase activating protein (GAP) accelerates nucleotide hydrolysis, switching off the GTPase. The remaining GDP-bound Rab can then participate in a new round of fusion. Rab interactions with effectors are likely to regulate vesicle targeting and membrane fusion in three ways. First, a Rab may specifically facilitate vectorial vesicle transport. Vesicles are transported from their site of origin to acceptor compartments likely through associations with cytoskeletal elements and transport motors. A protein has been identified with a domain structure that suggests a connection between the cytoskeleton and the Rabs. This protein, called Rabkinesin-6, contains a kinesin-like ATPase motor domain followed by a coiled-coil stalk region and a RBD that specifically binds Rab6 (Echard et al., 1998 ). An additional link with the cytoskeleton is provided by the Rab effector, Rabphilin-3A. Rabphilin-3A has been shown in vitro to interact with -actinin, an actin-bundling protein, but only when not bound to Rab3A (Kato et al., 1996 ). These results raise the intriguing possibility that Rab proteins regulate vesicle interactions with the cytoskeleton and thereby play an active role in targeting vesicles to their appropriate destinations. Second, Rab proteins may regulate membrane trafficking at the vesicle docking step. A number of Rab effectors, including Rabaptin-5, EEA1, Rabphilin-3 A, and Rim, may serve as molecular tethers. Each effector protein contains a RBD, followed by a linker region (some having the potential to form elongated coiled-coil structures), and a domain capable of interacting with a second Rab or the target membrane. Rabaptin-5, for example, contains two RBDs, one near the N terminus that specifically recognizes Rab4 and a second near the C terminus that binds Rab5 (Vitale et al., 1998 ). Both Rim, which is localized to the target membrane, and Rabphilin-3 A, which is localized to the vesicle, contain N-terminal RBDs and C-terminal Ca2+-binding C2 domains, implicating these effectors in synaptic vesicle localization or docking in response to Ca2+ influx (Wang et al., 1997 ). Tethering effectors may also recognize protein complexes on the acceptor membrane. Sec4p, a yeast Rab3A homolog, interacts with the exocyst (Guo et al., 1999 ), a complex of seven or more subunits that is assembled at sites of vesicle fusion along the plasma membrane. The exocyst complex may therefore function as a landmark for Rab/effector-mediated vesicle docking. Third, once a vesicle has become tethered to its fusion site, Rab proteins may selectively activate the SNARE fusion machinery. The mechanism of this activation is unknown but may involve direct interactions of Rabs or, more likely, their effectors with SNAREs. For example, Hrs-2 is a protein that binds to SNAP-25 and contains a Zn2+-fϊnger motif characteristic of Rab-binding proteins such as Rabphilin-3 A, Rim, EEA1, and Noc2, suggesting that Hrs-2 may form a physical link between Rabs and SNAREs (Bean et al., 1997). In addition, certain mutations in the syntaxin-binding protein Slylp, the Seclp homolog utilized in ER to Golgi trafficking, eliminate the requirement for Yptlp, a Rab protein that functions at this trafficking step (Dascher et al., 1991 ). Rabs may therefore regulate SNARE associations through Seel family members. In support of this idea, a Rab effector was recently found to interact with a vacuole Rab, a Seclp homolog, and a SNARE protein (Peterson et al., 1999 ), which suggests that this effector serves to connect Rab and SNARE function. In this way, Rabs and their effectors may facilitate the correct pairing of SNAREs. References: Dascher et al. (1991). Mol. Cell. Biol. 11, 872-885; Echard et al. (1998). Science. 279, 580-585; Geppert et al. (1998). Annu. Rev. Neurosci. 21, 75-95; Guoet al. (1999). EMBO J. 18, 1071-1080; Kato et al. (1996). J. Biol. Chem. 271, 31775-31778; Novick et al. (1997). Curr. Opin. Cell Biol. 9, 496-504; Peterson et al. (1999). Curr. Biol. 9, 159-162; Poirier et al. (1998). Nat. Struct. Biol. 5, 765-769; Vitale et al. (1998). EMBO J. 17, 1941-1951; Wang et al. (1997). Nature. 388, 593-598; Yang et al. (1999). J. Biol. Chem. 274, 5649-5653. Kinases Reversible posttranslational modifications of proteins are major means of regulating cellular activities. Among the various modifications that are carried out by the cells, the addition of phosphoryl groups to Ser/Thr or Tyr residues is the most important and widely used. The phosphorylation of proteins is accomplished by protein kinases, while the reverse reaction, the removal of phosphoryl groups, is carried out by phosphatases. Kinases / Phosphatases regulate key positions e.g. in the processes of cell proliferation, differentiation and communication/signaling. These processes must be tightly regulated in order to maintain a steady state level of cellular fate. Mis-regulation of kinase activities (or that of phosphatases) is made responsible for a multitude of disease processes such as oncogenesis, inflammatory processes, arteriosclerosis, and psoriasis. Protein kinases constitute the largest protein family that is currently known. Several hundred kinases have been identified already. Classically, kinases are subdivided into two classes based on the amino acid residues in their substrates that are phosphorylated by the particular enzymes. The kinases specifically add phosphoryl groups from adenosine triphosphate (ATP) or, less frequently, guanosine triphosphate (GTP), either to serine and/or threonine or to tyrosine residues of substrate proteins. An estimated 1,000 to 10,000 proteins present in a typical mammalian cell are believed to be regulated also by the action of protein kinases. Protein kinases are frequently integral parts of signaling cascades that transmit extracellular stimuli (e.g. hormones, neurotransmitters, growth- or differentiation factors) into the cell and result in various responses by the cells. The kinases play key roles in these cascades as they constitute a sort of 'molecular switches' turning on or off the activities of other enzymes and proteins, e.g. metabolic, regulatory, channels and pumps, receptors, cytoskeletal, transcription factors. The regulation of kinase activities is accomplished by various means: The best characterized example for the regulation via regulatory subunits is the cAMP-dependent protein kinase (PKA) which is also a prototype for second messenger activated protein kinases. This enzyme consists of a heterotetramer of two catalytic (C) and two regulatory (R) subunits. Upon binding of two molecules of second messenger (cAMP) in each R subunit, the catalytic subunits are released and active. Both of the catalytic and the regulatory subunits several isoforms exist. The combination of catalytic and regulatory subunits determines the localization of the holoenzyme and also the substrate spectrum that is available for phosphorylation. The consensus pattern necessary to be present in the substrate for PKA action is RRXS/T where X can be any amino acid. The casein kinase II comprises another examples for holoenzymes that consist of catalytic and regulatory subunits. Other kinases that are activated by second messengers are cGMP-dependent protein kinase and Protein kinase C (PKC) which is activated by diacylglycerol, which in turn is produced by phospholipases by cleavage of phosphatidylcholine. Receptor kinases usually consists of an extracellular domain which can bind effector molecules (e.g. growth factors and hormones) and transfer the stimulus to the intracellular domain of these proteins which usually is a protein tyrosine kinase. Other tyrosine kinases lack an extracellular domain but are associated with receptors which transfer the signal after effector binding by activating the associated protein kinase enzyme (e.g. Src kinase family; Src, Blk, Fgr, Fyn, Lck Lyn, Yes and Janus kinase family; Jakl-3, Tyk2). Dysfunction of kinases, e.g. caused by non-functioning regulation, can be the cause of inflammatory diseases and uncontrolled proliferation. v-Src which is a truncated version of the C-Src protooncogene tyrosine kinase is a classical example for this process as v-Src does not contain the regulatory domain of the cellular gene and is thus constitutively active. Several categories of proteins are coded for by clones of the invention within the overall group of "Signal transduction"and include, among others, the following: Neurocalcin (Recoverin): Neurocalcin is a Ca(2+)-binding protein with three putative Ca(2+)-binding domains (EF -hands). In cattle, 6 isoforms are differentially expressed in the central nervous system, retina and adrenal gland. Homology with recoverin indicates involvement in Ca2+ dependent activation of guanylate cyclase.. These proteins can find application in modulating/blocking the guanylate cyclase-pathway. Diseases associated (as potentially diagnostic, therapeutic, causative, and/or related, etc..) with these proteins include as reported by OMIN 1) autosomal dominant cone dystrophy (OMIN *600364); 2) cone dystrophy 3 (OMIN *600364); 3) cancer associated retinopathy (OMIN *179618). Clones in this category include: fbr2_23b21. Proteins with a WW Domain: Proteins that contain a WW domain which has been originally described as a short conserved region in a number of unrelated proteins, among them dystrophin, the gene responsible for Duchenne muscular dystrophy. The domain, which spans about 35 residues, is repeated up to 4 times in some proteins. It has been shown to bind proteins with particular proline-motifs, [AP]-P-P-[AP]-Y, and thus resembles somewhat SH3 domains. This domain is frequently associated with other domains typical for proteins in signal transduction processes. Examples of proteins containing the WW domain are Dystrophin, Utrophin, vertebrate YAP protein (binds the SH3 domain of the Yes oncoprotein), murine NEDD-4 (embryonic development and differentiation of the central nervous system), IQGAP (human GTPase activating protein acting on ras). Therefore these proteins should be involved in intracellular signal transduction. Diseases associated (as potentially diagnostic, therapeutic, causative, and/or related, etc...) with these proteins include as reported by OMIN 1) Muscular Dystrophy, Pseudohypertrophic Progressive Duchenne and Becker Types (OMIN *310200). Clones in this category include: fbr2_23nl6. Protein substrates for cAMP-dependent protein kinase: Acting as a choride channel or chloride channel inhibitor these proteins have been associated (as potentially diagnostic, therapeutic, causative, and/or related, etc..) as reported by OMIN with Cystic Fibrosis (OMIN #219700). Clones in this category include fbr2_82il7. Sphingosine kinase: Sphingosine kinase is a new type of lipid kinase, which is regulated by growth factors. The enzyme phosphorylates sphingosine, which subsequently exerts intracellular and extracellular actions. Intracellulary, sphingosine 1 -phosphate (SPP) promotes proliferation and inhibits apoptosis. In yeast, survival of cells exposed to heat shock indicates is dependent on SPP. Extracellulary, SPP inhibits cell motility and influences cell morphology, effects that appear to be mediated by the G protein-coupled receptor EDG1. These proteins have been associated (as potentially diagnostic, therapeutic, causative, and/or related, etc...) as reported by OMIN with Gaucher Disease, Type I (OMIN *230800). Clones in this category include fbr2_82m6. Vanilloid Receptors: VR1 seems to play an important role in the activation and sensitization of nociceptors. It is the receptor for e.g. capsaicin, a selective activator of nociceptors, a natural product of capsicum peppers. Related can find application as a target for the development of new nociception-modulating drugs. Clones in this category include tes3_20k2. RCCl (Regulator of chromosome condensation): RCCl (regulator of chromosome condensation) is a eukaryotic protein which binds to chromatin and interacts with ran, a nuclear GTP-binding protein. RCCl promotes the exchange of bound GDP with GTP, acting as a guanine-nucleotide dissociation stimulator. These proteins can find application in the regulation of gene expression by activition of nuclear GTP-binding proteins. The X-linked retinitis pigmentosa is a result of a defect GTPase regulator, which contains a RCCl -type repeat. OMIN also reports that RCCl has associations (as potentially diagnostic, therapeutic, causative, and or related, etc ..) with retinitis pigmentosa (OMIN *312610). Clones in this category include tes3_21d4. Ras inhibitor proteins: Ras is a signal transducting molecule involved in the receptor tyrosine kinase/RAS/Map kinase signalling cascade. Ras proteins bind GDP/GTP and show intrinsic GTPase activity. Mutations in ras, which change aa 12, 13 or 61 activate the potential of ras to transform cultured cells and are implicated in a variety of human tumours. Ras inhibitor proteins have been associated (as potentially diagnostic, therapeutic, causative, and/or related, etc...) with many disease processes as reported by OMIN including: 1) Tumors of the lung, breast, brain, pituitary, pancrase, bone, skin, bladder, kidney, ovary, prostate and lymphocyte, Melanoma (OMIN *600160); 2) X-linked non-specific mental retardation (OMIN * 300104); 3)adenomatouspolyposis of the colon (OMIN * 175100); 4) Beckwith-Wieddemann Syndrome (#130650); and 5) Major affective disorder 1 (OMIN * 125480). Clones in this category include utel_22g21. Mammalian proteins cornicon involving the EGF-receptor: Cornicon proteins are part of a signal transduction pathway involving the EGF-receptor. The EGF-receptor has been reported by OMIN to be associated (as potentially diagnostic, therapeutic, causative, and/or related, etc...) with the following diseases: 1) Familial hypercholesterolemia (OMIN 143890); 2) Leprechaunism (OMIN #246200); 3) Hemophilia B (OMIN *306900); 4) Ectodermal dysplasia 1; 5) Kartagenerer syndrome (OMIN *244400) and 6) Glioma of the brain (OMIN * 137800). ). Clones in this category include utel_22el2. Transmembrane proteins Membrane region prediction was effected using the ALOM2 software (Klein et al., 1985; version 2 by K. Nakai). Similar to many other methods, the Kyte & Doolitle (1982) amino acid hydrophobicity scale is used in ALOM2 as the primary variable for classifying sequences in terms of their localization. High prediction accuracy is achieved through the system of intelligent decision rules and the utilization of a carefully selected training data set. The method also generates reliability estimates which makes it possible to distinguish between membrane-spanning proteins (I, intrinsic) and globular proteins with regions of high hydrophobicity buried in the core. For a protein of length L, the block of length / with maximum hydrophobicity is found: where H, represents the hydrophobicity of an individual residue. Let P(I/maxΗ) and P(E/maxH) be the conditional probabilities that a protein is integral or peripheral, respectively, given its value of maximal hydrophobicity maxH, and let P(I) and P(E) be the prior probabilities of intrinsic and extrinsic membrane proteins estimated from the training set. Then a sequence is assigned to E if P(E/maxH) > P(I/maxH) or, after applying the Bayes rule, P(E)P(maxH/E) > P(I)P(maxH/I), where the conditional probabilities P(maxH E) and P(maxH I) can be determined based on the estimates of probability distributions of maxH in both groups. Discriminant analysis allows to simplify this task by calculating the odds P(E/MaxH):P(I/maxH) as e b , where b is the left-hand side of a linear or quadratic inequality. For example, for the window of length 17, the protein is allocated to the peripheral category E based on the empirically derived quadratic inequality: 1.05(maxH) 2 +12.30maxH+17.49 >0, whereas the optimal inequality for assigning membrane proteins (category I) is linear: -9.02maxH + 14.27 > 0 The odds parameter can be made more or less stringent. For example, one can require odds at least 1 : 10 for a protein to be classified as integral. This leads to higher selectivity but less sensitivity. The boundaries of membrane-spanning regions in putative membrane proteins are detected by means of an iterative procedure whereby the most hydrophobic region corresponding to the value maxH is considered to be membrane and removed from the sequence. The classification procedure is then repeated again for the remaining sequence, and, if such a protein is again classified as integral, the next most hydrophobic region is considered. Reference: Klein, P., Kanehisa, M., DeLisi, C. (1985) The detection and classification of membrane-spanning proteins. Biochem Biophys Ada 815: 468-476 Transcription factors Purified eukaryotic RNA polymerase II is unable to initiate promoter-specific transcription. A family of factors that collectively confer RNAPII promoter specificity is known as the general transcription factors (GTFs). They include the TATA-binding Protein (TBP) TFIIB, TFIIE, TFIIF and TFI IH. These factors are conserved among all eukaryotes. RNAPII complexes containing the entire set of GTFs or a subset of GTFs together with other proteins have been isolated from mammalian and yeast cells. Although purified RNAPII and GTFs are sufficient for promoter-specific initiation, this system fails to respond to activators. This is mediated by a further complex termed mediator complex which associates with the carboxy-terminal heptapeptide domain (CTD) of the largest subunit of RNAPII. Purification of human RNAPII complexes resulted in two distinct forms of human RNAPII after analysis of functional properties. One complex contained chromatin remodeling activities but was devoid of GTFs. The other complex did not contain factors that modify chromatin but contained a subset of SRB/mediator subunits and GTFs and other polypeptides that mediate transcriptional activation, a scenario similar to that reported for yeast. A complex designated NAT (~2O SU) for negative regulator of transcription contains RNAPII, Cdk8, homologs of the yeast mediator complex as well as Rgrl and SrblO/11 known as negative regulators of transcription. A complex with striking similar structural and functional properties to NAT has been identified designated SMCC (~15 SU) (SRB/mediator coactivator complex), that can also mediate transcriptional activation. The SMCC complex includes all reported NAT subunits including subunits of the TRAP complex. TRAP is a coactivator complex isolated on the basis of its interaction with the thyroid hormone receptor. Another coactivator complex DRIP, isolated on the basis of its ability to interact with the vitamin D3 receptor, contains novel subunits as well as subunits of NAT/SMCC and TRAP complexes. The effects of each of these coactivator complexes is dependent on the TFIID complex. It is not known if the T AF subunits of TFIID are required. It is likely that new coactivator complexes will be uncovered containing both novel and previously defined components. Beside the huge amount of transcription factors which can be part of the RNAIIP holoenzyme or the coactivator complexes there is an even larger quantity of specific transcription factors binding to promoter elements within the DNA sequences of a given gene leading to activation or repression of transcription. A broad range of cellular responses like differentiation, proliferation, cell death and others are elicited through activating or repressing the transcription of target genes. There are at least five superclasses of transcription factors: 1. Superclass contains members with characteristic basic domains: Members are: Leucine zipper factors, where the basic domain is followed by a leucine zipper of repeated leucine residues at every seventh position. The zipper mediates protein dimerization as a prerequisite for DNA-binding. Helix-loop-helix factors (bHLH) contain a DNA-binding basic region followed by a motif of two potential amphipathic alpha-helices connected by a loop of variable length also mediating dimerization. Factors with a combination of Helix-loop-helix and leucine zipper. Further members of this superclass are NF-1, RF-X, and bHSH like proteins. 2. Superclass comprises factors containing zinc-coordinating DNA-binding domains. Members are: Proteins with Cys4 zinc finger of nuclear receptor type, where two such motifs differing in size, composition and function are present in each receptor molecule. Each finger comprises 4 cysteine residues coordinating one zinc ion. The second half including the second cysteine pair has alpha-helix conformation and the helix of the first finger binds to the DNA through the major groove. The sequence between the first two cysteines of the second finger mediates dimerization upon DNA-binding. This class includes the steroid hormone receptors and the thyroid hormone receptor-like factors. Other diverse cys4 zinc fingers have a motif of GATA-type. Proteins with Cys2His2 zinc finger domain(s). Each finger comprises 2 cysteine and 2 histidine residues coordinating one zinc ion, and in some cases one histidine is replaced by another cysteine. The zinc ion is essential for DNA-binding. Proteins with Cys6 cysteine-zinc cluster(s). Six cysteine residues coordinate two zinc ions, i. e. two of the thiol groups are coordinating two zinc ions each. Present in many fungal regulators. Zinc fingers of alternating composition. 3. Superclass contains factors of helix-turn-helix type. Members are: Proteins with homeo domains. Homeo domains are three consecutive alpha-helix structures. Helix 3 contacts mainly the major groove of the DNA, some contacts at the minor groove are observed as well. Helix 2 and 3 resemble the helix-turn-helix structure of prokaryotic regulators. Proteins with Paired box domain(s). This is a DNA-binding domain of approximately 130 amino acid residues. Its N-terminal half is basic, its C-terminal half is highly charged in general. It probably comprises 3 alpha-helices. Proteins with Fork head / winged helix domain(s). This domain was identified by homology between HNF-3A and fkh. The domain comprises approx. 110 AA. Analysis of the crystal structure has revealed a compact structure of three alpha-helices, the third alpha-helix being exposed towards the major groove of the DNA. The domain also exerts minor groove contacts. Upon binding to DNA, it induces a bend of 13 degree. Heat shock factors Proteins with Tryptophan clusters. The tryptophan clusters comprise several tryptophan residues with a spacing of 12-21 amino acid residues; the subclass of myb-type DNA-binding domains typically exhibit a spacing of 19-21 amino acid residues. Proteins with TEA domain(s). The TEA domain has been identified as a region which is conserved among the transcription factors TEF-1, TEC1 and abaA. This domain in TEF-1 has been shown to interact with DNA, although two additional regions may also contribute to DNA-binding. It is predicted to fold into three alpha-helices, with a randomly coiled region of 16-18 amino acid residues between helices 1 and 2, and a short stretch between helices 2 and 3 of 3-8 residues. 4. Superclass contains beta-Scaffold Factors with Minor Groove Contacts Members are: Proteins with RHR (Rel homology) region. The structure of the Rel-type DBD exhibits a bipartite subdomain structure, each subdomain comprising a beta-barrel with five loops that form an extensive contact surface to the major groove of the DNA. Particularly, the first loop of the N-terminal subdomain (the highly conserved recognition loop) performs contacts with the recognition element on the DNA, but other loops are involved. The fact that the main DNA-contacts are made through loops has been suggested to provide a high degree of flexibility in binding to a range of different target sequences. Augmenting interactions are achieved by two alpha-helices within the N-terminal Part that form strong minor groove contacts to the A/T-rich center of the B- element. In p65, the sequence between both alpha-helices is much shorter and even helix 2 is truncated. The second, C-terminal domain is necessary mainly for protein dimerization. p53 proteins MADS (MCMl-agamous-deficiens-SRF) box proteins. Proteins of this class comprise a region of homology. The DNA-binding domain also comprises the dimerization capability. In the DNA-bound dimer (shown for SRF), two antiparallel amphipathic alpha-helices (alpha- I), form a coiled coil and are oriented approximately parallel on the minor groove. These helices make minor and major groove contacts, the N-terminal extensions form minor groove contacts. The bound DNA is bent and wrapped around the protein. It exhibits a compressed minor groove in the center and widened minor groove in the flanks. Beta-Barrel alpha-helix transcription factors. TATA-binding proteins HMG proteins Proteins of this class comprise a region of homology with the chromosomal non- histone HMG proteins such as HMG1. This region comprises the DNA-binding domain which in some instances such as HMG1 mediates sequence-unspecific, in other cases such LEF-1 sequence-specific binding to DNA. This domain exhibits a typical L-shaped conformation made up of 3 alpha-helices and an extended N-terminal extension of the first helix. The latter together with helix 1, which contains a kink, form the long arm of the L, whereas helices 1 and 2 form the short arm. Binding to the minor groove induces a sharp bending of the DNA by more than 90 degree, away from the bound protein. The overall topology of the DNA-protein complexes resembles somewhat that of the TBP-TATA box complex. Heteromeric CCAAT factors Proteins with Grainyhead domain(s) Cold-shock domain factors. Cold-shock domain proteins are characterized by a highly conserved region first found in prokaryotic cold-shock proteins. This domain is a single- stranded nucleic acid-binding structure interacting with DNA or RNA. It consists of an antiparallel five-stranded beta-barrel, the strands of which are connected by turns and loops. Within this structure, a three-stranded beta-strand contains a conserved RNA-binding motif, RNP1. Not all CSD proteins are transcription factors. Those which specifically bind to a certain sequence are termed Y-box proteins. Proteins of this class were previously called protamine-like domain proteins because of having a highly positively charged domain with interspersed proline residues. Proteins with Runt homology domain The members of this transcription factor class have been identified on the basis of their homology to a defined region within the Drosophilia protein Runt. The runt domain is part of the DNA-binding domain of these factors. It consists mainly of beta-strands, does not contain alpha-helical regions and seems to be most similar to the palm domain found in DNA polymerase beta (rat). 5. Superclass contains other transcription factors like Copper fist proteins. HMGKY). STAT. Pocket domain proteins and Ap2/EREBP-related factors. The classification of transcription factors originates from TRANSFAC database: http: //transfac.gbf.de/TRANSFAC/ Reference: Heinemeyer Several categories of proteins are coded for by clones of the invention within the overall group of "Transcription Factors".and include, among others, the following: Dcoh: Dcoh is a bifunctional protein, complexed with biopterin. It serves as dimerization cofactor of hepatocyte nuclear factor- 1 and catalyzes the dehydration of the biopterin cofactor of phenylalanine hydroxylase. The Dcoh protein has been reported by OMIN to be associated (as potentially diagnostic, therapeutic, causative, and/or related, etc...) with the following diseases: 1) hyperphenylalanemia (OMIN 126090, #264070). Clones in this category include fkd2_46kl2. Signal transducing proteins: Beta-transducin subunits of G-proteins contain WD-40 repeats. The beta subunits seem to be required for the replacement of GDP by GTP as well as for membrane anchoring and receptor recognition. Due to the zinc finger the novel protein seems to be a new molecule involved in signal transduction and transcription. These proteins have been reported by OMIN to be associated (as potentially diagnostic, therapeutic, causative, and or related, etc...) with the following diseases: 1) essential hypertension (OMIN *139130). Clones in this category include utel_H2. * * * The invention, therefore, specifically contemplates the following assemblages of materials, which track the above- identified fourteen functional groupings, that are useful in practicing the profiling aspects of the invention. One type of assemblage is nucleic acid- based and can include the following groupings of sequences and their derivatives: all sequences; human fetal brain sequences; brain derived sequences; human fetal kidney library sequences; kidney derived sequences; human mammary carcinoma library sequences; mammary carcinoma derived sequences; human testis library sequences; testes derived sequences; cell cycle genes; cell structure and motility genes; differentiation and development genes; intracellular transport and trafficking genes; metabolism genes; nucleic acid management genes; signal transduction genes; transmembrane protein genes; and transcription factor genes. Other assemblages contain proteins or their corresponding antibodies or antibody fragments, divided along the same groupings. Database Applications Because they are human genes and gene products, the inventive molecules are useful as members of a database. Such a database may be used, for example, in drug discovery and rationale drug design or in testing the novelty and non-obviousness of newly sequenced materials. In addition, they are particularly suited in designing variants for the profiling (and other) applications described herein. Hence, the following discussion of electronic embodiments applies equally to such variants, which, naturally, will be generated and stored using a computer using known methodologies. Accordingly, one aspect of the invention contemplates a database of at least one of the inventive sequences stored on computer readable media. Again, the individual sequences may be grouped with regard to the individual functional and structural groups mentioned above. While the individual sequences of a database may exist in printed form, they are preferably in electronic form, as in an ascii or a text file. They may also exist as word processing files or they may be stored in database applications like DB2, Sybase, Oracle, GCG and GenBank. One skilled in the art will understand the range of applications suitable for using and storing the electronic embodiments of the invention. "Computer readable media" refers to any medium which can be read and accessed by a computer. These include: magnetic storage media, like floppy discs, hard drives and magnetic tape; optical storage media, like CD-ROM; electrical storage media, like RAM and ROM; and hybrids of these categories, like magnetic/optical storage media. One skilled in the art will readily understand the scope of computer readable media and how to implement them. Biological Activities and Assays for Implementing Therapeutic and Diagnostic Applications This section provides assays for biological activity that are useful in characterizing and quantifying the biological activity of the inventive molecules and their derivatives, which is relevant to the pharmacological effects of the inventive molecules. As used in this section, it will be understood that "protein" may also refer to the inventive antibodies (including fragments). Cytokine and Cell Proliferation/Differentiation Activity A protein of the present invention may exhibit cytokine, cell proliferation (either inducing or inhibiting) or cell differentiation (either inducing or inhibiting) activity or may induce production of other cytokines in certain cell populations. Many protein factors discovered to date, including all known cytokines, have exhibited activity in one or more factor dependent cell proliferation assays, and hence the assays serve as a convenient confirmation of cytokine activity. The activity of a protein of the present invention is evidenced by any one of a number of routine factor dependent cell proliferation assays for cell lines including, without limitation, 32D, DA2, DA1G, T10, B9, B9/11, BaF3, MC9/G, M + (preB M + ), 2E8, RB5, DAI, 123, T1165, HT2, CTLL2, TF-1, Mo7e and CMK. The activity of a protein of the invention may, among other means, be measured by the following methods: Assays for T-cell or thymocyte proliferation include without limitation those described in: Current Protocols in Immunology, Ed by J. E. Coligan, A. M. Kruisbeek, D. H. Margulies, E. M. Shevach, W. Strober, Pub. Greene Publishing Associates and Wiley- Interscience (Chapter 3, In Vitro assays for Mouse Lymphocyte Function 3.1-3.19; Chapter 7, Immunologic studies in Humans); Takai et al., J. Immunol. 137:3494-3500, 1986; Bertagnolli et al., J. Immunol. 145:1706-1712, 1990; Bertagnolli et al., Cellular Immunology 133:327-341, 1991; Bertagnolli, et al., I. Immunol. 149:3778-3783, 1992; Bowman et al., I. Immunol. 152:1756-1761, 1994. Assays for cytokine production and/or proliferation of spleen cells, lymph node cells or thymocytes include, without limitation, those described in: Polyclonal T cell stimulation, Kruisbeek, A. M. and Shevach, E. M. In Current Protocols in Immunology. J. E. e.a. Coligan eds. Vol 1 pp. 3.12.1-3.12.14, John Wiley and Sons, Toronto. 1994; and Measurement of mouse and human interleukin gamma , Schreiber, R. D. In Current Protocols in Immunology. J. E. e.a. Coligan eds. Vol 1 pp. 6.8.1-6.8.8, John Wiley and Sons, Toronto. 1994. Assays for proliferation and differentiation of hematopoietic and lymphopoietic cells include, without limitation, those described in: Measurement of Human and Murine Interleukin 2 and Interleukin 4, Bottomly, K., Davis, L. S. and Lipsky, P. E. In Current Protocols in Immunology. J. E. e.a. Coligan eds. Vol 1 pp. 6.3.1-6.3.12, John Wiley and Sons, Toronto. 1991; deVries et al., J. Exp. Med. 173: 1205-1211, 1991; Moreau et al., Nature 336:690-692, 1988; Greenberger et al., Proc. Natl. Acad. Sci. U.S.A. 80:2931- 2938, 1983; Measurement of mouse and human interleukin 6-Nordan, R. In Current Protocols in Immunology. J. E. e.a. Coligan eds. Vol 1 pp. 6.6.1-6.6.5, John Wiley and Sons, Toronto. 1991; Smith et al., Proc. Natl. Aced. Sci. U.S.A. 83: 1857-1861, 1986; Measurement of human Interleukin 11-Bennett, F., Giannotti, J.; Clark, S. C. and Turner, K. J. In Current Protocols in Immunology. J. E. e.a. Coligan eds. Vol 1 pp. 6.15.1 John Wiley and Sons, Toronto. 1991; Measurement of mouse and human Interleukin 9-Ciarletta, A., Giannotti, J., Clark, S. C. and Turner, K. J. In Current Protocols in Immunology. J. E. e.a. Coligan eds. Vol 1 pp. 6.13.1, John Wiley and Sons, Toronto. 1991. Assays for T-cell clone responses to antigens (which will identify, among others, proteins that affect APC-T cell interactions as well as direct T-cell effects by measuring proliferation and cytokine production) include, without limitation, those described in: Current Protocols in Immunology, Ed by J. E. Coligan, A. M. Kruisbeek, D. H. Margulies, E. M. Shevach, W Strober, Pub. Greene Publishing Associates and Wiley- Interscience (Chapter 3, In Vitro assays for Mouse Lymphocyte Function; Chapter 6, Cytokines and their cellular receptors; Chapter 7, Immunologic studies in Humans); Weinberger et al., Proc. Natl. Acad. Sci. USA 77:6091-6095, 1980; Weinberger et al., Eur. J. Immun. 11:405-411, 1981; Takai et al., J. Immunol. 137:3494-3500, 1986; Takai et al., J. Immunol. 140:508-512, 1988. Immune Stimulating or Suppressing Activity A protein of the present invention may also exhibit immune stimulating or immune suppressing activity, including without limitation the activities for which assays are described herein. A protein may be useful in the treatment of various immune deficiencies and disorders (including severe combined immunodeficiency (SOD)), e.g., in regulating (up or down) growth and proliferation of T and/or B lymphocytes, as well as effecting the cytolytic activity of NK cells and other cell populations. These immune deficiencies may be genetic or be caused by vital (e.g., HIV) as well as bacterial or fungal infections, or may result from autoimmune disorders. More specifically, infectious diseases causes by viral, bacterial, fungal or other infection may be treatable using a protein of the present invention, including infections by HIV, hepatitis viruses, herpesviruses, mycobacteria, Leishmania spp., malaria spp. and various fungal infections such as candidiasis. Of course, in this regard, a protein of the present invention may also be useful where a boost to the immune system generally may be desirable, i.e., in the treatment of cancer. Autoimmune disorders which may be treated using a protein of the present invention include, for example, connective tissue disease, multiple sclerosis, systemic lupus erythematosus, rheumatoid arthritis, autoimmune pulmonary inflammation, Guillain-Barre syndrome, autoimmune thyroiditis, insulin dependent diabetes mellitis, myasthenia gravis, graft-versus-host disease and autoimmune inflammatory eye disease. Such a protein of the present invention may also to be useful in the treatment of allergic reactions and conditions, such as asthma (particularly allergic asthma) or other respiratory problems. Other conditions, in which immune suppression is desired (including, for example, organ transplantation), may also be treatable using a protein of the present invention. Using the proteins of the invention it may also be possible to modify immune responses, in a number of ways. Down regulation may be in the form of inhibiting or blocking an immune response already in progress or may involve preventing the induction of an immune response. The functions of activated T cells may be inhibited by suppressing T cell responses or by inducing specific tolerance in T cells, or both. Immunosuppression of T cell responses is generally an active, non-antigen-specific, process which requires continuous exposure of the T cells to the suppressive agent. Tolerance, which involves inducing non-responsiveness or anergy in T cells, is distinguishable from immunosuppression in that it is generally antigen-specific and persists after exposure to the tolerizing agent has ceased. Operationally, tolerance can be demonstrated by the lack of a T cell response upon reexposure to specific antigen in the absence of the tolerizing agent. Down regulating or preventing one or more antigen functions (including without limitation B lymphocyte antigen functions (such as, for example, B7)), e.g., preventing high level lymphokine synthesis by activated T cells, will be useful in situations of tissue, skin and organ transplantation and in graft-versus-host disease (GVHD). For example, blockage of T cell function should result in reduced tissue destruction in tissue transplantation. Typically, in tissue transplants, rejection of the transplant is initiated through its recognition as foreign by T cells, followed by an immune reaction that destroys the transplant. The administration of a molecule which inhibits or blocks interaction of a B7 lymphocyte antigen with its natural ligand(s) on immune cells (such as a soluble, monomeric form of a peptide having B7-2 activity alone or in conjunction with a monomeric form of a peptide having an activity of another B lymphocyte antigen (e.g., B7- 1, B7-3) or blocking antibody), prior to transplantation can lead to the binding of the molecule to the natural ligand(s) on the immune cells without transmitting the corresponding costimulatory signal. Blocking B lymphocyte antigen function in this matter prevents cytokine synthesis by immune cells, such as T cells, and thus acts as an immunosuppressant. Moreover, the lack of costimulation may also be sufficient to anergize the T cells, thereby inducing tolerance in a subject. Induction of long-term tolerance by B lymphocyte antigen-blocking reagents may avoid the necessity of repeated administration of these blocking reagents. To achieve sufficient immunosuppression or tolerance in a subject, it may also be necessary to block the function of a combination of B lymphocyte antigens. The efficacy of particular blocking reagents in preventing organ transplant rejection or GVHD can be assessed using animal models that are predictive of efficacy in humans. Examples of appropriate systems which can be used include allogeneic cardiac grafts in rats and xenogeneic pancreatic islet cell grafts in mice, both of which have been used to examine the immunosuppressive effects of CTLA4Ig fusion proteins in vivo as described in Lenschow et al., Science 257:789-792 (1992) and Turka et al., Proc. Natl. Acad. Sci USA, 89:11102-11105 (1992). In addition, murine models of GVHD (see Paul ed., Fundamental Immunology, Raven Press, New York, 1989, pp. 846-847) can be used to determine the effect of blocking B lymphocyte antigen function in vivo on the development of that disease. Blocking antigen function may also be therapeutically useful for treating autoimmune diseases. Many autoimmune disorders are the result of inappropriate activation of T cells that are reactive against self tissue and which promote the production of cytokines and autoantibodies involved in the pathology of the diseases. Preventing the activation of autoreactive T cells may reduce or eliminate disease symptoms. Administration of reagents which block costimulation of T cells by disrupting receptor: ligand interactions of B lymphocyte antigens can be used to inhibit T cell activation and prevent production of autoantibodies or T cell-derived cytokines which may be involved in the disease process. Additionally, blocking reagents may induce antigen-specific tolerance of autoreactive T cells which could lead to long-term relief from the disease. The efficacy of blocking reagents in preventing or alleviating autoimmune disorders can be determined using a number of well-characterized animal models of human autoimmune diseases. Examples include murine experimental autoimmune encephalitis, systemic lupus erythmatosis in MRL/lpr/lpr mice or NZB hybrid mice, murine autoimmune collagen arthritis, diabetes mellitus in NOD mice and BB rats, and murine experimental myasthenia gravis (see Paul ed., Fundamental Immunology, Raven Press, New York, 1989, pp. 840-856). Upregulation of an antigen function (preferably a B lymphocyte antigen function), as a means of up regulating immune responses, may also be useful in therapy. Upregulation of immune responses may be in the form of enhancing an existing immune response or eliciting an initial immune response. For example, enhancing an immune response through stimulating B lymphocyte antigen function may be useful in cases of viral infection. In addition, systemic viral diseases such as influenza, the common cold, and encephalitis might be alleviated by the administration of stimulatory forms of B lymphocyte antigens systemically. Alternatively, anti-vital immune responses may be enhanced in an infected patient by removing T cells from the patient, costimulating the T cells in vitro with viral antigen- pulsed APCs either expressing a peptide of the present invention or together with a stimulatory form of a soluble peptide of the present invention and reintroducing the in vitro activated T cells into the patient. Another method of enhancing anti-viral immune responses would be to isolate infected cells from a patient, transfect them with a nucleic acid encoding a protein of the present invention as described herein such that the cells express all or a portion of the protein on their surface, and reintroduce the transfected cells into the patient. The infected cells would now be capable of delivering a costimulatory signal to, and thereby activate, T cells in vivo. In another application, up regulation or enhancement of antigen function (preferably B lymphocyte antigen function) may be useful in the induction of tumor immunity. Tumor cells (e.g., sarcoma, melanoma, lymphoma, leukemia, neuroblastoma, carcinoma) transfected with a nucleic acid encoding at least one peptide of the present invention can be administered to a subject to overcome tumor-specific tolerance in the subject. If desired, the tumor cell can be transfected to express a combination of peptides. For example, tumor cells obtained from a patient can be transfected ex vivo with an expression vector directing the expression of a peptide having B7-2-like activity alone, or in conjunction with a peptide having B7-l-like activity and/or B7-3-like activity. The transfected tumor cells are returned to the patient to result in expression of the peptides on the surface of the transfected cell. Alternatively, gene therapy techniques can be used to target a tumor cell for transfection in vivo. The presence of the peptide of the present invention having the activity of a B lymphocyte antigen(s) on the surface of the tumor cell provides the necessary costimulation signal to T cells to induce a T cell mediated immune response against the transfected tumor cells. In addition, tumor cells which lack MHC class I or MHC class II molecules, or which fail to reexpress sufficient mounts of MHC class I or MHC class II molecules, can be transfected with nucleic acid encoding all or a portion of (e.g. , a cytoplasmic-domain truncated portion) of an MHC class I alpha chain protein and beta 2 microglobulin protein or an MHC class II alpha chain protein and an MHC class II beta chain protein to thereby express MHC class I or MHC class II proteins on the cell surface. Expression of the appropriate class I or class II MHC in conjunction with a peptide having the activity of a B lymphocyte antigen (e.g., B7-1, B7-2, B7-3) induces a T cell mediated immune response against the transfected tumor cell. Optionally, a gene encoding an antisense construct which blocks expression of an MHC class II associated protein, such as the invariant chain, can also be cotransfected with a DNA encoding a peptide having the activity of a B lymphocyte antigen to promote presentation of tumor associated antigens and induce tumor specific immunity. Thus, the induction of a T cell mediated immune response in a human subject may be sufficient to overcome tumor-specific tolerance in the subject. The activity of a protein of the invention may, among other means, be measured by the following methods: Suitable assays for thymocyte or splenocyte cytotoxicity include, without limitation, those described in: Current Protocols in Immunology, Ed by J. E. Coligan, A. M. Kruisbeek, D. H. Margulies, E. M. Shevach, W. Strober, Pub. Greene Publishing Associates and Wiley-Interscience (Chapter 3, In Vitro assays for Mouse Lymphocyte Function 3.1-3.19; Chapter 7, Immunologic studies in Humans); Herrmann et al., Proc. Natl. Acad. Sci. USA 78:2488-2492, 1981; Herrmann et al., J. Immunol. 128: 1968-1974, 1982; Handa et al., J. Immunol. 135:1564-1572, 1985; Takai et al., I. Immunol. 137:3494- 3500, 1986; Takai et al., J. Immunol. 140:508-512, 1988; Herrmann et al., Proc. Natl. Acad. Sci. USA 78:2488-2492, 1981; Herrmann et al., J. Immunol. 128:1968-1974, 1982; Handa et al., J. Immunol. 135: 1564-1572, 1985; Takai et al., J. Immunol. 137:3494-3500, 1986; Bowmanet al., J. Virology 61:1992-1998; Takai et al., J. Immunol. 140:508-512, 1988; Bertagnolli et al. , Cellular Immunology 133:327-341, 1991; Brown et al., J. Immunol. 153:3079-3092, 1994. Assays for T-cell-dependent immunoglobulin responses and isotype switching (which will identify, among others, proteins that modulate T-cell dependent antibody responses and that affect Thl/Th2 profiles) include, without limitation, those described in: Maliszewski, J. Immunol. 144:3028-3033, 1990; and Assays for B cell function: In vitro antibody production, Mond, J. J. and Brunswick, M. In Current Protocols in Immunology. J. E. e.a. Coligan eds. Vol 1 pp. 3.8.1-3.8.16, John Wiley and Sons, Toronto. 1994. Mixed lymphocyte reaction (MLR) assays (which will identify, among others, proteins that generate predominantly Thl and CTL responses) include, without limitation, those described in: Current Protocols in Immunology, Ed by J. E. Coligan, A. M. Kruisbeek, D. H. Margulies, E. M. Shevach, W. Strober, Pub. Greene Publishing Associates and Wiley-Interscience (Chapter 3, In Vitro assays for Mouse Lymphocyte Function 3.1-3.19; Chapter 7, Immunologic studies in Humans); Takai et al., J. Immunol. 137:3494-3500, 1986; Takai et al., J. Immunol. 140:508-512, 1988; Bertagnolli et al., J. Immunol. 149:3778-3783, 1992. Dendritic cell-dependent assays (which will identify, among others, proteins expressed by dendritic cells that activate naive T-cells) include, without limitation, those described in: Guery et al., J. Immunol. 134:536-544, 1995; Inaba et al., Journal of Experimental Medicine 173:549-559, 1991; Macatonia et al. , Journal of Immunology 154:5071-5079, 1995; Porgador et al., Journal of Experimental Medicine 182:255-260, 1995; Nair et al., Journal of Virology 67:4062-4069, 1993; Huang et al., Science 264:961- 965, 1994; Macatonia et al., Journal of Experimental Medicine 169:1255-1264, 1989; Bhardwaj et al., Journal of Clinical Investigation 94:797-807, 1994; and Inaba et al., Journal of Experimental Medicine 172:631-640, 1990. Assays for lymphocyte survival/apoptosis (which will identify, among others, proteins that prevent apoptosis after superantigen induction and proteins that regulate lymphocyte homeostasis) include, without limitation, those described in: Darzynkiewicz et al., Cytometry 13:795-808, 1992; Gorczyca et al., Leukemia 7:659-670, 1993; Gorczyca et al., Cancer Research 53: 1945-1951, 1993; Itoh et al., Cell 66:233-243, 1991; Zacharchuk, Journal of Immunology 145:4037-4045, 1990; Zamai et al., Cytometry 14:891-897, 1993; Gorczyca et al., International Journal of Oncology 1:639-648, 1992. Assays for proteins that influence early steps of T-cell commitment and development include, without limitation, those described in: Antica et al., Blood 84:111-117, 1994; Fine et al., Cellular Immunology 155:111-122, 1994; Galy et al., Blood 85:2770-2778, 1995; Toki et al., Proc. Nat. Acad Sci. USA 88:7548-7551, 1991. Hematopoiesis Regulating Activity A protein of the present invention may be useful in regulation of hematopoiesis and, consequently, in the treatment of myeloid or lymphoid cell deficiencies. Even marginal biological activity in support of colony forming cells or of factor-dependent cell lines indicates involvement in regulating hematopoiesis, e.g. in supporting the growth and proliferation of erythroid progenitor cells alone or in combination with other cytokines, thereby indicating utility, for example, in treating various anemias or for use in conjunction with irradiation/chemotherapy to stimulate the production of erythroid precursors and/or erythroid cells; in supporting the growth and proliferation of myeloid cells such as granulocytes and monocytes/macrophages (i.e., traditional CSF activity) useful, for example, in conjunction with chemotherapy to prevent or treat consequent myelo- suppression; in supporting the growth and proliferation of megakaryocytes and consequently of platelets thereby allowing prevention or treatment of various platelet disorders such as thrombocytopenia, and generally for use in place of or complimentary to platelet transfusions; and/or in supporting the growth and proliferation of hematopoietic stem cells which are capable of maturing to any and all of the above-mentioned hematopoietic cells and therefore find therapeutic utility in various stem cell disorders (such as those usually treated with transplantation, including, without limitation, aplastic anemia and paroxysmal nocturnal hemoglobinuria), as well as in repopulating the stem cell compartment post irradiation/chemotherapy, either in-vivo or ex-vivo (i.e., in conjunction with bone marrow transplantation or with peripheral progenitor cell transplantation (homologous or heterologous)) as normal cells or genetically manipulated for gene therapy. The activity of a protein of the invention may, among other means, be measured by the following methods: Suitable assays for proliferation and differentiation of various hematopoietic lines are cited above. Assays for embryonic stem cell differentiation (which will identify, among others, proteins that influence embryonic differentiation hematopoiesis) include, without limitation, those described in: Johansson et al. Cellular Biology 15:141-151, 1995; Keller et al., Molecular and Cellular Biology 13:473-486, 1993; McClanahan et al., Blood 81:2903- 2915, 1993. Assays for stem cell survival and differentiation (which will identify, among others, proteins that regulate lympho-hematopoiesis) include, without limitation, those described in: Methylcellulose colony forming assays, Freshney, M. G. In Culture of Hematopoietic Cells. R. I. Freshney, et al. eds. Vol pp. 265-268, Wiley-Liss, Inc., New York, N.Y. 1994; Hirayama et al., Proc. Natl. Acad. Sci. USA 89:5907-5911, 1992; Primitive hematopoietic colony forming cells with high proliferative potential, McNiece, I. K. and Briddell, R. A. In Culture of Hematopoietic Cells. R. I. Freshney, et al. eds. Vol pp. 23- 39, Wiley-Liss, Inc., New York, N.Y. 1994; Neben et al., Experimental Hematology 22:353-359, 1994; Cobblestone area forming cell assay, Ploemacher, R. E. In Culture of Hematopoietic Cells. R. I. Freshney, et al. eds. Vol pp. 1-21, Wiley-Liss, Inc., New York, N.Y. 1994; Long term bone marrow cultures in the presence of stromal cells, Spooncer, E., Dexter, M. and Allen, T. In Culture of Hematopoietic Cells. R. I. Freshney, et al. eds. Vol pp. 163-179, Wiley-Liss, Inc., New York, N.Y. 1994; Long term culture initiating cell assay, Sutherland, H. J. In Culture of Hematopoietic Cells. R. I. Freshney, et al. eds. Vol pp. 139-162, Wiley-Liss, Inc., New York, N.Y. 1994. Tissue Growth Activity A protein of the present invention also may have utility in compositions used for bone, cartilage, tendon, ligament and/or nerve tissue growth or regeneration, as well as for wound healing and tissue repair and replacement, and in the treatment of burns, incisions and ulcers. A protein of the present invention, which induces cartilage and/or bone growth in circumstances where bone is not normally formed, has application in the healing of bone fractures and cartilage damage or defects in humans and other animals. Such a preparation employing a protein of the invention may have prophylactic use in closed as well as open fracture reduction and also in the improved fixation of artificial joints. De novo bone formation induced by an osteogenic agent contributes to the repair of congenital, trauma induced, or oncologic resection induced craniofacial defects, and also is useful in cosmetic plastic surgery. A protein of this invention may also be used in the treatment of periodontal disease, and in other tooth repair processes. Such agents may provide an environment to attract bone-forming cells, stimulate growth of bone-forming cells or induce differentiation of progenitors of bone-forming cells. A protein of the invention may also be useful in the treatment of osteoporosis or osteoarthritis, such as through stimulation of bone and/or cartilage repair or by blocking inflammation or processes of tissue destruction (collagenase activity, osteoclast activity, etc.) mediated by inflammatory processes. Another category of tissue regeneration activity that may be attributable to the protein of the present invention is tendon/ligament formation. A protein of the present invention, which induces tendon/ligament-like tissue or other tissue formation in circumstances where such tissue is not normally formed, has application in the healing of tendon or ligament tears, deformities and other tendon or ligament defects in humans and other animals. Such a preparation employing a tendon ligament-like tissue inducing protein may have prophylactic use in preventing damage to tendon or ligament tissue, as well as use in the improved fixation of tendon or ligament to bone or other tissues, and in repairing defects to tendon or ligament tissue. De novo tendon ligament-like tissue formation induced by a composition of the present invention contributes to the repair of congenital, trauma induced, or other tendon or ligament defects of other origin, and is also useful in cosmetic plastic surgery for attachment or repair of tendons or ligaments. The compositions of the present invention may provide environment to attract tendon- or ligament-forming cells, stimulate growth of tendon- or ligament-forming cells, induce differentiation of progenitors of tendon- or ligament-forming cells, or induce growth of tendon/ligament cells or progenitors ex vivo for return in vivo to effect tissue repair. The compositions of the invention may also be useful in the treatment of tendonitis, carpal tunnel syndrome and other tendon or ligament defects. The compositions may also include an appropriate matrix and/or sequestering agent as a carrier as is well known in the art. The protein of the present invention may also be useful for proliferation of neural cells and for regeneration of nerve and brain tissue, i.e. for the treatment of central and peripheral nervous system diseases and neuropathies, as well as mechanical and traumatic disorders, which involve degeneration, death or trauma to neural cells or nerve tissue. More specifically, a protein may be used in the treatment of diseases of the peripheral nervous system, such as peripheral nerve injuries, peripheral neuropathy and localized neuropathies, and central nervous system diseases, such as Alzheimer's, Parkinson's disease, Huntington's disease, amyotrophic lateral sclerosis, and Shy-Drager syndrome. Further conditions which may be treated in accordance with the present invention include mechanical and traumatic disorders, such as spinal cord disorders, head trauma and cerebro vascular diseases such as stroke. Peripheral neuropathies resulting from chemotherapy or other medical therapies may also be treatable using a protein of the invention. Proteins of the invention may also be useful to promote better or faster closure of non-healing wounds, including without limitation pressure ulcers, ulcers associated with vascular insufficiency, surgical and traumatic wounds, and the like. It is expected that a protein of the present invention may also exhibit activity for generation or regeneration of other tissues, such as organs (including, for example, pancreas, liver, intestine, kidney, skin, endothelium), muscle (smooth, skeletal or cardiac) and vascular (including vascular endothelium) tissue, or for promoting the growth of cells comprising such tissues. Part of the desired effects may be by inhibition or modulation of fibrotic scarring to allow normal tissue to regenerate. A protein of the invention may also exhibit angiogenic activity. A protein of the present invention may also be useful for gut protection or regeneration and treatment of lung or liver fibrosis, reperfusion injury in various tissues, and conditions resulting from systemic cytokine damage. A protein of the present invention may also be useful for promoting or inhibiting differentiation of tissues described above from precursor tissues or cells; or for inhibiting the growth of tissues described above. The activity of a protein of the invention may, among other means, be measured by the following methods: Assays for tissue generation activity include, without limitation, those described in: International Patent Publication No. WO95/ 16035 (bone, cartilage, tendon); International Patent Publication No. WO95/05846 (nerve, neuronal); International Patent Publication No. WO91/07491 (skin, endothelium). Assays for wound healing activity include, without limitation, those described in: Winter, Epidermal Wound Healing, pps. 71-112 (Maibach, H. I. and Rovee, D. T., eds.), Year Book Medical Publishers, Inc., Chicago, as modified by Eaglstein and Mertz, J. Invest. Dermatol 71:382-84 (1978). Activin/Inhibin Activity A protein of the present invention may also exhibit activin- or inhibin-related activities. Inhibins are characterized by their ability to inhibit the release of follicle stimulating hormone (FSH), while activins and are characterized by their ability to stimulate the release of follicle stimulating hormone (FSH). Thus, a protein of the present invention, alone or in heterodimers with a member of the inhibin alpha family, may be useful as a contraceptive based on the ability of inhibins to decrease fertility in female mammals and decrease spermatogenesis in male mammals. Administration of sufficient amounts of other inhibins can induce infertility in these mammals. Alternatively, the protein of the invention, as a homodimer or as a heterodimer with other protein subunits of the inhibin- beta group, may be useful as a fertility inducing therapeutic, based upon the ability of activin molecules in stimulating FSH release from cells of the anterior pituitary. See, for example, U.S. Pat. No. 4,798,885. A protein of the invention may also be useful for advancement of the onset of fertility in sexually immature mammals, so as to increase the lifetime reproductive performance of domestic animals such as cows, sheep and pigs. " The activity of a protein of the invention may, among other means, be measured by the following methods: Assays for activin/ inhibin activity include, without limitation, those described in: Vale et al., Endocrinology 91:562-572, 1972; Ling et al., Nature 321:779-782, 1986; Vale et al., Nature 321:776-779, 1986; Mason et al., Nature 318:659-663, 1985; Forage et al., Proc. Natl. Acad. Sci. USA 83:3091-3095, 1986. Chemotactic/Chemokinetic Activity A protein of the present invention may have chemotactic or chemokinetic activity (e.g., act as a chemokine) for mammalian cells, including, for example, monocytes, fibroblasts, neutrophils, T-cells, mast cells, eosinophils, epithelial and/or endothelial cells. Chemotactic and chemokinetic proteins can be used to mobilize or attract a desired cell population to a desired site of action. Chemotactic or chemokinetic proteins provide particular advantages in treatment of wounds and other trauma to tissues, as well as in treatment of localized infections. For example, attraction of lymphocytes, monocytes or neutrophils to tumors or sites of infection may result in improved immune responses against the tumor or infecting agent. A protein or peptide has chemotactic activity for a particular cell population if it can stimulate, directly or indirectly, the directed orientation or movement of such cell population. Preferably, the protein or peptide has the ability to directly stimulate directed movement of cells. Whether a particular protein has chemotactic activity for a population of cells can be readily determined by employing such protein or peptide in any known assay for cell chemotaxis. The activity of a protein of the invention may, among other means, be measured by the following methods: Assays for chemotactic activity (which will identify proteins that induce or prevent chemotaxis) consist of assays that measure the ability of a protein to induce the migration of cells across a membrane as well as the ability of a protein to induce the adhesion of one cell population to another cell population. Suitable assays for movement and adhesion include, without limitation, those described in: Current Protocols in Immunology, Ed by J. E. Coligan, A. M. Kruisbeek, D. H. Marguiles, E. M. Shevach, W. Strober, Pub. Greene Publishing Associates and Wiley-Interscience (Chapter 6.12, Measurement of alpha and beta Chemokines 6.12.1-6.12.28; Taub et al. J. Clin. Invest. 95:1370-1376, 1995; Lind et al. APMIS 103:140-146, 1995; Muller et al Eur. J. Immunol. 25: 1744-1748; Gruber et al. J. of Immunol. 152:5860-5867, 1994; Johnston et al. J. of Immunol. 153: 1762-1768, 1994. Hemostatic and Thrombolytic Activity A protein of the invention may also exhibit hemostatic or thrombolytic activity. As a result, such a protein is expected to be useful in treatment of various coagulation disorders (including hereditary disorders, such as hemophilias) or to enhance coagulation and other hemostatic events in treating wounds resulting from trauma, surgery or other causes. A protein of the invention may also be useful for dissolving or inhibiting formation of thromboses and for treatment and prevention of conditions resulting therefrom (such as, for example, infarction of cardiac and central nervous system vessels (e.g., stroke). The activity of a protein of the invention may, among other means, be measured by the following methods: Assay for hemostatic and thrombolytic activity include, without limitation, those described in: Linet et al., J. Clin. Pharmacol. 26: 131-140, 1986; Burdick et al., Thrombosis Res. 45:413-419, 1987; Humphrey et al., Fibrinolysis 5:71-79 (1991); Schaub, Prostaglandins 35:467-474, 1988. Receptor/Ligand Activity A protein of the present invention may also demonstrate activity as receptors, receptor ligands or inhibitors or agonists of receptor/ligand interactions. Examples of such receptors and ligands include, without limitation, cytokine receptors and their ligands, receptor kinases and their ligands, receptor phosphatases and their ligands, receptors involved in cell-cell interactions and their ligands (including without limitation, cellular adhesion molecules (such as selectins, integrins and their ligands) and receptor/ligand pairs involved in antigen presentation, antigen recognition and development of cellular and humoral immune responses). Receptors and ligands are also useful for screening of potential peptide or small molecule inhibitors of the relevant receptor/ligand interaction. A protein of the present invention (including, without limitation, fragments of receptors and ligands) may themselves be useful as inhibitors of receptor/ligand interactions. The activity of a protein of the invention may, among other means, be measured by the following methods: Suitable assays for receptor-ligand activity include without limitation those described imCurrent Protocols in Immunology, Ed by J. E. Coligan, A. M. Kruisbeek, D. H. Margulies, E. M. Shevach, W. Strober, Pub. Greene Publishing Associates and Wiley- Interscience (Chapter 7.28, Measurement of Cellular Adhesion under static conditions 7.28.1-7.28.22), Takai et al., Proc. Natl. Acad. Sci. USA 84:6864-6868, 1987; Bierer et al., J. Exp. Med. 168:1145-1156, 1988; Rosenstein et al., J. Exp. Med. 169:149-160 1989; Stoltenborg et al., J. Immunol. Methods 175:59-68, 1994; Stitt et al., Cell 80:661- 670, 1995. Anti-Inflammatory Activity Proteins of the present invention may also exhibit anti-inflammatory activity. The anti-inflammatory activity may be achieved by providing a stimulus to cells involved in the inflammatory response, by inhibiting or promoting cell-cell interactions (such as, for example, cell adhesion), by inhibiting or promoting chemotaxis of cells involved in the inflammatory process, inhibiting or promoting cell extravasation, or by stimulating or suppressing production of other factors which more directly inhibit or promote an inflammatory response. Proteins exhibiting such activities can be used to treat inflammatory conditions including chronic or acute conditions), including without limitation intimation associated with infection (such as septic shock, sepsis or systemic inflammatory response syndrome (SIRS)), ischemia-reperfusion injury, endotoxin lethality, arthritis, complement- mediated hyperacute rejection, nephritis, cytokine or chemokine-induced lung injury, inflammatory bowel disease, Crohn's disease or resulting from over production of cytokines such as TNF or IL-1. Proteins of the invention may also be useful to treat anaphylaxis and hypersensitivity to an antigenic substance or material. Tumor Inhibition Activity In addition to the activities described above for immunological treatment or prevention of tumors, a protein of the invention may exhibit other anti-tumor activities. A protein may inhibit tumor growth directly or indirectly (such as, for example, via ADCC). A protein may exhibit its tumor inhibitory activity by acting on tumor tissue or tumor precursor tissue, by inhibiting formation of tissues necessary to support tumor growth (such as, for example, by inhibiting angiogenesis), by causing production of other factors, agents or cell types which inhibit tumor growth, or by suppressing, eliminating or inhibiting factors, agents or cell types which promote tumor growth. Other Activities A protein of the invention may also exhibit one or more of the following additional activities or effects: inhibiting the growth, infection or function of, or killing, infectious agents, including, without limitation, bacteria, viruses, fungi and other parasites; effecting (suppressing or enhancing) bodily characteristics, including, without limitation, height, weight, hair color, eye color, skin, fat to lean ratio or other tissue pigmentation, or organ or body part size or shape (such as, for example, breast augmentation or diminution, change in bone form or shape); effecting biorhythms or caricadic cycles or rhythms; effecting the fertility of male or female subjects; effecting the metabolism, catabolism, anabolism, processing, utilization, storage or elimination of dietary fat, lipid, protein, carbohydrate, vitamins, minerals, cofactors or other nutritional factors or component(s); effecting behavioral characteristics, including, without limitation, appetite, libido, stress, cognition (including cognitive disorders), depression (including depressive disorders) and violent behaviors; providing analgesic effects or other pain reducing effects; promoting differentiation and growth of embryonic stem cells in lineages other than hematopoietic lineages; hormonal or endocrine activity; in the case of enzymes, correcting deficiencies of the enzyme and treating deficiency-related diseases; treatment of hyperproliferative disorders (such as, for example, psoriasis); immunoglobulin-like activity (such as, for example, the ability to bind antigens or complement); and the ability to act as an antigen in a vaccine composition to raise an immune response against such protein or another material or entity which is cross-reactive with such protein. Particular Applications for Certain Clones The following sets out a non-exclusive list of applications for certain embodiments of the invention. In the interest of economy, applications relevant to multiple embodiments are not duplicated in this list. Other embodiments described in below have similar characteristics, as described therein. The artisan is directed, therefore, to this section for similar descriptions of the functions of other embodiment. Testes htes3_l 5c24: The new protein can find application in modulation of 2-hydroxyacid dehydrogenases-dependent pathways and as a new enzyme for biotechnologic production processes. htes3_15i5: The new protein can find application in modulating the structure of the human spermatozoa radia spoke head and modulation of sperm motility in men. htes3_15kl 1 : The novel protein contains a protein kinase ATP-binding region signature and a serine/threonine protein kinase active-site signature. The new protein can find application in modulation of intracellular signal pathways dependent on this kinase. htes3_17nl2: The new protein can find application in modulating/blocking the expression of SOX-controlled genes. htes3_20k2: The new protein can find application as a target for the development of new nociception-modulating drugs. htes3_20ml8: The new protein can find application in modulation of mitochondrial DNA replication and maintenance. htes3_20d4: The new protein can find application in the regulation of gene expression by activition of nuclear GTP-binding proteins. The X-linked retinitis pigmentosa is a result of a defect GTPase regulator, which contains a RCCl -type repeat. htes3_21jl5: NY-CO-33 is a protein recognised by autologous antibodies of human colon cancer patients. The novel protein contains 4 C2H2 Zinc fingers and is a new putativ transcription factor. The new protein can find application in modulating/blocking the expression of genes controlled by this transcription factor. The new protein can find application in modulating chromosome transport in mitosis and meiosis and modulation of cell division. htes3_26g22: The new protein can find application in modulating chromosome transport in mitosis and meiosis and modulation of cell division. The novel TBP- binding protein is considered to participate in transcription regulation through the interaction with TBP. The new protein can find application in modulation of gene transcription. htes3_21116: The new protein can find application in modulation of protein translocation into the endoplasmic reticulum. htes3_27dl : The novel protein can find application in modulation of ubiquitin- and protein metabolism in cells. htes3_2ml8: The novel protein can find application as multifunctional nuclease / exoribonuclease. htes3_35b4: The new protein can find application in modulation of the mitotic spindle. htes3_35b5: The novel protein can find application in modulating the v-ATPase activity in endocytic and secretory organelles. htes3_35e21 : Due to the close relationship to human interleukin-7, the novel interleukin is expected to act as a new growth factor for human B lineage cells. Additionally, the protein should induce the gene rearrangement of the T-cell receptor repertoire, leading to thymocyte commitment, and subsequently induce both cytotoxic T-cell- and lymphocyte-activated killer cells. This new interleukin could find clinical application in a variety of conditions of hematolymphopoietic failure and different tumours, because of its recruitment of B cell lineage cells, cytotoxic T-cell- and lymphocyte-activated killer cells. htes3_35kl6: Therefore it is a new fatty acid-Co A synthetasese/ligase with unknown substrate. The new protein can find application in modulation of fatty acid metabolism and as a new enzyme for biotechnologic production processes. htes3_35nl2: The new protein can find application in modulation of ADP-transport and energy metabolism in cells/mitochondria. htes3_35n9: The new protein can find application in modulation of carboxylester metabolism and as a new enzyme for biotechnologic production processes. htes3_35p22: The novel protein is closely raleted to human tre-2 and other enzymes involved in the degradation of ubiquitinated proteins. The human tre-2 oncogene encodes a deubiquitinating enzyme, indicating a role for the ubiquitin system in mammalian growth control. The novel protein can find application in cancer diagnostics and treatment, and in regulating protein stability and growth control via regulation of ubiquitination. htes3_4h6: The novel kinesin protein can find application in modulating the function of kinesin and modulating intracellular transport via/on microtubules. htes3_72kl5: FGDl -related F-actin-binding protein (Farbin/FGDl) is a novel F-actin- binding protein. The gene locus fgdl seems to be responsible for faciogenital dysplasia or Aarskog-Scott syndrome. Frabin binds F-actin and shows F-actin-cross- linking activity. Overexpression of frabin in Swiss 3T3 cells and COS7 cells induces cell shape change and c-Jun N-terminal kinase activation, as described for FGDl. Because FGDl has been shown to serve as a GDP/GTP exchange protein for Cdc42 small G protein, it is likely that frabin is a direct linker between Cdc42 and the actin cytoskeleton. Cdc42p is an esin yeast, Cdc42p transduces signals to the actin cytoskeleton to initiate and maintain polarized growth and to mitogen-activated protein morphogenesis. In mammalian cells, Cdc42p regulates a variety of actin- dependent events and induces the JNK/SAPK protein kinase cascade, which leads to the activation of transcription factors within the nucleus. The novel protein seems to be the human orthologue of rat frabin. The new protein can find application in modulating of cell structure and motility as well as modulation of the JNK/SAPK pathway. htes3_72pl6: As Mem3, the novel protein is similar to yeast VPS (vacuolar protein sorting) 35. The null allele of VPS35 results in yeast in a differential defect in the sorting of vacuolar carboxypeptidase Y (CPY), proteinase A (PrA), proteinase B (PrB), and alkaline phosphatase (ALP). The new protein can find application in modulation the sorting of proteins into different compartments. htes3_7b22: The novel protein is related to paramyosin, a major structural component of thick filaments and invertebrate muscle. Paramyosins are promising antigens for immunization against several parasites, such as Schistosoma mansoni. The new protein can find application in modulating cell adhesion/motility and membrane/cyto skeleton structure and dynamic. htes3_7j3: The new protein is closely related to C-Takl and therefore should be involved in cell-cycle regulation, too. The new protein can find application in modulating/blocking the cell cycle. htes3_7p9: The nuclear domain (ND)10 also described as POD or Kr bodies is involved in the development of acute promyelocytic leukemia and virus-host interactions. The NDP52 protein is part of this complex structure. In vivo, NDP52 is transcribed in all human tissues, but is redistributed upon viral infection and interferon treatment. ND10 plays an important role in the viral life cycle. The novel protein is similar to NDP52. It contains three leucine zippers and a RGD cell attachment site. This protein seems to be a novel part of the ND819) complex. The new protein can find application in modulation of viral infections and tumour events. htes3_8ml0: The poly(A)-binding protein (PABP) binds to the messenger (mRNA) 3'-poly(A) tail found on most eukaryotic mRNAs and together with the poly(A) tail has been implicated in governing the stability and the translation of mRNA. The new protein can find application in modulation of mRNA translation and processing/stability. Kidney hfkd2_24bl5: The new protein can find application in modulation of hexose metabolism pathways and as a new enzyme for biotechnologic production processes. hfkd2_24n20: The new protein seems to be part of the signalling pathway between tyrosine kinases and the membrane/cyto skeleton. The new protein can find application in modulating cell adhesion/motility and membrane/cyto skeleton structure and dynamics. hfkd2_3ol7: The new protein can find application in modulation of the respiratory electron transport chain pathways of mitochondria. hfkd2_46j20: The new protein can find application in modulating the homoprotocatechuate degradative pathway and as a enzyme for biotechnologic production processes. hfkd2_46kl9: The new protein can find application in modulating/blocking the expression of genes controlled by the hepatocyte nuclear factor- 1. hfkd2_46m4: SARI proteins are involved in vesicular transport between the endoplasmic reticulum and the Golgi apparatus. hfkd2_46kl4: rab6 is a ubiquitous ras-like GTPase involved in intra-Golgi transport. The new protein can find application in modulating the transport of vesicles inside the Golgi apparatus. Uterus Associated: hutel_18il9: The SREBP-2 protein is embedded in the membranes of the nucleus and endoplasmic reticulum. In cholesterol-depleted cells the proteins are cleaved to release soluble NH2 -terminal fragments that enter the nucleus and activate genes encoding the low density lipoprotein receptor and enzymes of cholesterol synthesis. The new protein is a putative transcription factor capable of protein-protein interaction via a lim domain and additionally shows similarity to the common sunflower transcription factor SF3. hute 1_1811 : The novel protein is similar to several 40S ribosomal proteins and therefore seems to part of the corresponding ribosome sub-unit. hute l_19g22: The new protein can find application in modulation of tissue- calcification, especially the uterus. hutel_19hl7: The new protein can find application in modulating the response of cells to oxysterols. hute l_20bl9: The novel protein seems to be a novel enzyme with sarcosine oxidase activity. The new protein can find application in modulation of sarcosine metabolism and as a new enzyme for biotechnologic production processes. hute l_20g21 : The novel protein seems to be a new ras inhibitor protein. The new protein can find application in modulating/blocking ras dependent signal transduction pathways. hute l_20hl3: The novel protein is a new human alpha-adaptin. The new protein can find application in modulating endocytosis and vesicle trafficking in cells. hute l_20ml 1 : The new protein can find application in modulating/blocking the activity of protein phosphatase- 1 and in modulating the cell cycle. hute l_20m24: This protein is a putative mannosyl transferase that is involved in the assembly of the core oligosaccharide Glc3Man9GlcNAc2. The new protein can find application in modulation of glycosylation of proteins and as a new enzyme for biotechnologic production processes. hute l_22el2: The new protein can find application in modulating the cornichon modulated signal transduction way and also the EGF receptor signaling processes. hute l_23el3: The novel protein contains a serine protease of the subtilase family with an aspartic acid-containing active site. The new protein can find application in modulation of proteinase activity in cells and as a new enzyme for proteomics and biotechnologic production processes. hutel_24j6: The new protein can find application in modulation of cell-cell-adhesion. hutel_24h3: The new protein can find application as a useful marker for chondro- osteogenic cell differentiation and for the modulation of chondro-osteogenic cell differentiation. Fetal Brain: hfbr2_16cl6: The new protein can find application in modulating/blocking of cyto skeleton-membrane protein interaction. hfbr2_23b21 : The new protein can find application in modulating/blocking the guanylate cyclase-pathway. hfbr2_23bl0: The new protein can find application in modulation of splicing. hfbr2_2b5: The novel protein contains the typical (xxG)n repeat of collagen proteins and a Pfam von Willebrand factor type A domain. Therefore, the protein seems to be a new collagen alpha chain. The new protein can find application in modulation of connective tissue, bone and cartilage development and maintainance. hfbr2_2cl7: The new protein can find application in modulating/blocking G-protein- dependent pathways. hfbr2_2dl5: The new protein can find application in modulating early spermatogenesis. hfbr2_2il7: The new protein can find clinical application in modulating the transport of glycoproteins inside cells, especially of the LDL receptor. hfbr2_2kl4: Tumour-suppressor genes are known to be involved in the control of cell growth and division, interacting with proteins which control the cell cycle. The N33 gene is significantly methylated in tumour cells, a mechanism by which tumor- suppressor genes are inactivated in cancer. In addition, the novel protein contains a RGD cell attachment site. Therefore the novel protein is a new putative tumour- suppressor gene. hfbr_3cl8: RNA helicases comprise a large family of proteins that are involved in basic biological systems such as nuclear and mitochondrial splicing processes, RNA editing, rRNA processing, translation initiation, nuclear mRNA export, and mRNA degradation. RNA helicases are essential factors in cell development and differentiation, and some of them play a role in transcription and replication of viral single-stranded RNA genomes. The members of the largest subgroup, the DEAD and DEAH box proteins, exhibit a strong dependence of the unwinding activity on ATP hydrolysis. The novel protein contains a DEAD-box and is a new member of this subgroup. hfbr_3g8: The new protein can find application modulating NAT assembly and action and therefore be important in metabolism of drugs and environmental mutagens. hfbr2_62bl 1 : The rac small GTPase is associated with type-I phosphatidylinositol 4- phosphate 5-kinase and regulating the production of phosphatidylinositol 4,5- bisphosphate. The new protein is expected to activate p21 rac -related small GTPases. hfbr2_62ol7: The new protein can find application in modulation of cholesterol binding and transport by LDL-receptors and LDL-binding proteins. hfbr_6b24: The new protein can find application in modulation of rhamnose metabolism and as a new enzyme for biotechnologic production processes. hfbr_72bl8: The new protein can find application in modulating DNA repair and mutagenesis. hfbr_78c4: The new protein can find application in modulating/blocking the response of cells to interferons. hfbr_78k24: These enzymes are involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins. The new protein can find application in modulation of protein stability /degradation in cells. hfbr_82e4: The new protein can find clinical application in modulating/blocking calmodulin-mediated pathways in human neuronal cells. VARIANTS OF THE INVENTIVE DNA MOLECULES Variants in General "Variants," according to the invention, include DNA and/or protein molecules that resemble, structurally and/or functionally, those set forth in herein. Variants may be isolated from natural sources ("homologs"), may be entirely synthetic or may be based in part on both natural and synthetic approaches. The section set forth below presents various structural and functional characteristics of molecules within the invention. Preferred molecules are characterized by a combination of one or more of these characteristics. For instance, some preferred molecules are described with reference to at least two structural characteristics, while others may be described with reference to at least one structural and at least one functional characteristic. It will be recognized by the skilled artisan that structure ultimately defines function, i.e. the functions of the molecules described herein derives from the structures of those molecules. Accordingly, the structural variants described below that bear the closest structural relationship (as variously defined below) to the inventive molecules are the variants that most likely will preserve biological function. This relationship between structure and function will guide the skilled artisan in identifying the preferred embodiments of the invention. Splicing Variants It is well-known that eukaryotic structural genes are comprised of both protein coding and non-coding portions. When the messenger RNA is transcribed from the DNA template, it contains introns, which are non-coding, and exons, which are coding. In order to form a translation competent mRNA, the introns must be "spliced" out of this initial pre mRNA. Specific sequences within the pre mRNA represent "splice junctions" that direct the cellular splicing machinery to the appropriate position. The splice junctions are loosely conserved sequence regions of the pre mRNA, which almost invariably begin with GT and end with AG (DNA perspective). The 5' end of the splice junction typically contains about nine somewhat conserved residues, for example, C/AAGTA/G AGT . The 3' end usually contains a pyrimidine rich stretch of at least about 11 nucleotides, followed by NC/TAGG. Splicing occurs before the GT and after the AG. Mount, Nucleic Acids Res. 10:459-72 (1982). Interestingly, exons often correspond to discrete functional domains of the protein product. The intron exon arrangement thus creates a linear array of nucleotides which can be correlated to discrete, and often interchangeable, functional protein fragments. Go, Nature 291:90-92 (1981); Branden et al , EMBO J. 3:1307-10 (1984). This linear arrangement creates the possibility of generating multiple different full length proteins by rearranging the order of the different functional portions in the array. For example, if a set of exons are arranged 1-2-3-4, where (-) represents the introns separating the exons, a splicing event need not simply produce 1234, but may produce 123, 134, 124 and so on. Production of different mRNA products in this way is commonly called "alternative splicing. " Andreadiset al. , Ann. Rev. Cell Biol. 3:207-42 (1987). Some of the present DNA molecules can be represented in modular fashion in terms of their coding regions. Essentially, these modules are exons (though each "exon" may in fact be made up of several exons), which may be combined in different ways to form a variety of different DNA molecules, each encoding a different functional protein. Splicing variants are indicated below. Degenerate Variants One aspect of the present invention provides "degenerate variants" of the nucleic acid fragments of the present invention. A "degenerate variant" is a nucleotide fragment which differs from those of inventive molecules by nucleotide sequence, but due to the degeneracy of the genetic code, encodes an identical polypeptide sequence. Given the known relationship between DNA sequences and the proteins they encode, degenerate variants typically are described by reference to this relationship. It is well known that the degeneracy of the genetic code results in many possible DNA sequences which encode a particular protein. Indeed, of the three bases which comprise an amino acid- encoding triplet, the third position, and often the second, almost always may vary. This fact alone allows for a class of variant DNA molecules which encode protein sequences identical to those disclosed herein, yet have about 30% sequence variation. In other words, the variant DNA molecules are about 70% identical to the inventive DNAs, having no additional or deleted sequences. Thus, one aspect of the invention provides degenerate variant DNA molecules encoding the inventive protein sequences. In one embodiment, these variants have at least about 70% sequence identity with the DNA molecules described herein. In a preferred embodiment, these variants have at least about 80% sequence identity to the inventive molecules. In a more preferred embodiment these variants have at least about 90% sequence identity with the inventive molecules. Conservative Amino Acid Variants Variants according to the invention also may be made that conserve the overall molecular structure of the encoded proteins. Given the properties of the individual amino acids comprising the disclosed protein products, some rational substitutions will be recognized by the skilled worker. Amino acid substitutions, i.e. "conservative substitutions," may be made, for instance, on the basis of similarity in polarity, charge, solubility, hydrophobicity, hydrophilicity, and/or the amphipathic nature of the residues involved. For example: (a) nonpolar (hydrophobic) amino acids include alanine, leucine, isoleucine, valine, proline, phenylalanine, tryptophan, and methionine; (b) polar neutral amino acids include glycine, serine, threonine, cysteine, tyrosine, asparagine, and glutamine; (c) positively charged (basic) amino acids include arginine, lysine, and histidine; and (d) negatively charged (acidic) amino acids include aspartic acid and glutamic acid. Substitutions typically may be made within groups (a)-(d). In addition, glycine and proline may be substituted for one another based on their ability to disrupt α-helices. Similarly, certain amino acids, such as alanine, cysteine, leucine, methionine, glutamic acid, glutamine, histidine and lysine are more commonly found in α-helices, while valine, isoleucine, phenylalanine, tyrosine, tryptophan and threonine are more commonly found in β-pleated sheets. Glycine, serine, aspartic acid, asparagine, and proline are commonly found in turns. Some preferred substitutions may be made among the following groups: (i) S and T; (ii) P and G; and (iii) A, V, L and I. Given the known genetic code, and recombinant and synthetic DNA techniques, the skilled scientist readily can construct DNAs encoding the conservative amino acid variants. As used herein, "sequence identity" between two polypeptide sequences indicates the percentage of amino acids that are identical between the sequences. "Sequence similarity" indicates the percentage of amino acids that either are identical or that represent conservative amino acid substitutions. Functionally Equivalent Variants Yet another class of DNA variants within the scope of the invention may be described with reference to the product they encode. As shown below, some of the inventive DNA molecules encode a protein having a degree of homology with known proteins, or protein domains. It is expected, therefore, that they will have some or all of the requisite functional features of such molecules. These "functionally equivalent variants" products are characterized by the fact that they are functionally equivalent, with respect to biological activity, to certain known molecules. The instant invention provides information on common structural motifs, including consensus sequences that will guide the artisan in constructing functionally equivalent variants. It will be understood that the motifs, identified for each inventive protein, may be modified within the identified consensus sequences. Thus, the invention contemplates the proteins disclosed herein that contain variability in the consensus sequences identified, and the invention further contemplates the full range of nucleic acids encoding them, and the complements of those nucleic acids. Hybridizing Variants DNA variants within the invention also may be described by reference to their physical properties in hybridization. One skilled in the field will recognize that DNA can be used to identify its complement and, since DNA is double stranded, its equivalent or homolog, using nucleic acid hybridization techniques. It will also be recognized that hybridization can occur with less than 100% complementarity. However, given appropriate choice of conditions, hybridization techniques can be used to differentiate among DNA sequences based on their structural relatedness to a particular probe. For guidance regarding such conditions see, for example, Sambrook et al , 1989, MOLECULAR CLONING, A LABORATORY MANUAL, Cold Spring Harbor Press, N.Y. ; and Ausubel et al, 1989, CURRENT PROTOCOLS IN MOLECULAR BIOLOGY, Green Publishing Associates and Wiley Interscience, N.Y. Structural relatedness between two polynucleotide sequences can be expressed as a function of "stringency" of the conditions under which the two sequences will hybridize with one another. As used herein, the term "stringency" refers to the extent that the conditions disfavor hybridization. Stringent conditions strongly disfavor hybridization, and only the most structurally related molecules will hybridize to one another under such conditions. Conversely, non-stringent conditions favor hybridization of molecules displaying a lesser degree of structural relatedness. Hybridization stringency, therefore, directly correlates with the structural relationships of two nucleic acid sequences. The following relationships are useful in correlating hybridization and relatedness (where T m is the melting temperature of a nucleic acid duplex): a. T m = 69.3 + 0.41(G+C)% b. The T m of a duplex DNA decreases by 1°C with every increase of 1 % in the number of mismatched base pairs. c μ 2 - (T m ) μ , = 18.5 lθg 10 μ2/μl where μl and μ2 are the ionic strengths of two solutions. Hybridization stringency is a function of many factors, including overall DNA concentration, ionic strength, temperature, probe size and the presence of agents which disrupt hydrogen bonding. Factors promoting hybridization include high DNA concentrations, high ionic strengths, low temperamres, longer probe size and the absence of agents that disrupt hydrogen bonding. Hybridization usually is done in two stages. First, in the "binding" stage, the probe is bound to the target under conditions favoring hybridization. Stringency is usually controlled at this stage by altering the temperature. For high stringency, the temperature is usually between 65°C and 70°C, unless short (<20 nt) oligonucleotide probes are used. A representative hybridization solution comprises 6X SSC, 0.5% SDS, 5X Denhardt's solution and lOOμg of non-specific carrier DNA. See Ausubel et al , supra, section 2.9, supplement 27 (1994). Of course many different, yet functionally equivalent, buffer conditions are known. Where the degree of relatedness is lower, a lower temperature may be chosen. Low stringency binding temperatures are between about 25°C and 40°C. Medium stringency is between at least about 40°C to less than about 65°C. High stringency is at least about 65°C. Second, the excess probe is removed by washing. It is at this stage that more stringent conditions usually are applied. Hence, it is this "washing" stage that is most important in determining relatedness via hybridization. Washing solutions typically contain lower salt concentrations. One exemplary medium stringency solution contains 2X SSC and 0.1 % SDS. A high stringency wash solution contains the equivalent (in ionic strength) of less than about 0.2X SSC, with a preferred stringent solution containing about 0. IX SSC. The temperatures associated with various stringencies are the same as discussed above for "binding. " The washing solution also typically is replaced a number of times during washing. For example, typical high stringency washing conditions comprise washing twice for 30 minutes at 55° C. and three times for 15 minutes at 60° C. The present invention includes nucleic acid molecules that hybridize to the inventive molecules under high stringency binding and washing conditions. More preferred molecules (from an mRNA perspective) are those that are at least 50 % of the length of any one of those depicted in below. Particularly preferred molecules are at least 75 % of the length of those molecules. Substitutions, Insertions, Additions and Deletions In a general sense, the preferred DNA variants of the invention are those that retain the closest relationship, as described by "sequence identity" to the inventive DNA molecules. According to another aspect of the invention, therefore, substitutions, insertions, additions and deletions of defined properties are contemplated. It will be recognized that sequence identity between two polynucleotide sequences, as defined herein, generally is determined with reference to the protein coding region of the sequences. Thus, this definition does not at all limit the amount of DNA, such as vector DNA, that may be attached to the molecules described herein. Preferred DNA sequence variants include molecules encoding proteins sharing some or all of any relevant biological activity of the native molecule. In creating these variants, the skilled worker will be guided by reference to the protein structure. First, insertions and deletions in any recognized functional domain, above, generally should be avoided, except as noted below in the section entitled "Proteins," where this domain is discussed in detail. Alterations in such domains usually will be limited to conservative amino acid substitutions. In addition, where insertions and deletions are desired, this may be accomplished at the N- and/or C-terminus of the protein molecule (or the corresponding coding regions of the DNA). If insertions or deletions are made within the protein, deletions of major structural features usually should be avoided. Thus, a preferred place to make insertion or deletion variants is in non-structural regions, such as linker regions between two alpha helices. "Substitutions" generally refer to alterations in the DNA sequence which do not change its overall length, but only alter one or more nucleotide positions, substituting one for another in the common sense of the word. One class of preferred substitutions, "degenerate substitutions, " are those that do not alter the encoded amino acid sequence. Some subsitutions retains 50%, 55%, 60% or 65% identity. Preferred substitutions retain at least about 70% identity, more preferably at least 70% or 75% identity, with the inventive DNAs. Some more preferred molecules have at least about 80% identity, more preferably at least 80% or 85% identity. Particularly preferred DNAs share at least about 90% identity, more preferably at least 90% or 95% identity. "Insertions," unlike substitutions, alter the overall length of the DNA molecule, and thus sometimes the encoded protein. Insertions add extra nucleotides to the interior (not the 5' or 3' ends) of the subject DNAs. Preferred insertions are made with reference to the protein sequence encoded by the DNA. Thus, it is most preferred to provide an insertion in the DNA at a location that corresponds to an area of the encoded protein which lacks structure. For instance, it typically would not be beneficial, if the preservation of biological activity is desired, to provide an insertion within an alpha-helical region or a beta-pleated sheet. Accordingly, non-structural areas, such as those containing helix-breaking glycines and proline residues, are most preferred sites of insertion. Other preferred sites of insertion are the splice sites, which are indicated above in the description of the inventive DNA molecules. While the optimal size of insertions will vary depending upon the site of insertion and its effect on the overall conformation of the encoded protein, some general guides are useful. Generally, the total insertions (irrespective of their number) should not add more than about 30% (or preferably not more than 30%) to the overall size of the encoded protein. More preferably, the insertion adds less than about 10-20% (yet more preferably 10-20%) in size, with less than about 10% being most preferred. The number of insertions is limited only by the number of suitable insertions sites, and secondarily by the foregoing size preferences. "Additions," like insertions, also add to the overall size of the DNA molecule, and usually the encoded protein. However, instead of being made within the molecule, they are made on the 5' or 3' end, usually corresponding to the N- or C- terminus of the encoded protein. Unlike deletions, additions are not very size-dependent. Indeed, additions may be of virtually any size. Preferred additions, however, do not exceed about 100% of the size of the native molecule. More preferably, they add less than about 60 to 30% to the overall size, with less than about 30% being most preferred. "Deletions" diminish the overall size of the DNA and, therefore, also reduce the size of the protein encoded by that DNA. Deletions may be made from either end of the molecule or internal to it. Typical preferred deletions remove discrete structural features of the encoded protein. For example, some deletions will comprise the deletion of one or more exons which may define a structural feature. Preferred deletions remove less than about 30% of the size of the subject molecule. More preferred deletions remove less than about 20% and most preferred deletions remove less than about 10% . Computer-Defined Variants and Definition of "Sequence Identity " In general, both the DNA and protein molecules of the invention can be defined with reference to "sequence identity. " As used herein, "sequence identity" refers to a comparison made between two molecules using, for example, the standard Smith- Waterman algorithm that is well known in the art. Some molecules have at lease about 50%, 55% or 60% identity. Preferred molecules are those having at least about 65% sequence identity, more preferably at least 65% or 70% sequence identity. Other preferred molecules have at least about 80%, more preferably at least 80% or 85%, sequence identity. Particularly preferred molecules have at least about 90% sequence identity, more preferably at least 90% sequence identity. Most preferred molecules have at least about 95 % , more preferably at least 95 % , sequence identity. As used herein, two nucleic acid molecules or proteins are said to "share significant sequence identity" if the two contain regions which possess greater than 85 % sequence (amino acid or nucleic acid) identity. "Sequence identity" is defined herein with reference the Blast 2 algorithm, which is available at the NCBI (http://www.ncbi.nlm.nih.gov/BLAST), using default parameters. References pertaining to this algorithm include: those found at http://www.ncbi.nlm.nih.gov/BLAST/blast_references.html; Altschul, S.F., Gish, W., Miller, W., Myers, E.W. & Lipman, D.J. (1990) "Basic local alignment search tool." J. Mol. Biol. 215:403-410; Gish, W. & States, D.J. (1993) "Identification of protein coding regions by database similarity search." Nature Genet. 3:266-272; Madden, T.L., Tatusov, RL. & Zhang, J. (1996) "Applications of network BLAST server" Meth. Enzymol. 266:131-141; Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D.J. (1997) "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs." Nucleic Acids Res. 25:3389-3402; and Zhang, J. & Madden, T.L. (1997) "PowerBLAST: A new network BLAST application for interactive or automated sequence analysis and annotation." Genome Res. 7:649-656. METHODS OF MAKING VARIANTS It will be recognized that variants of the inventive molecules can be constructed in several different ways. For example, they may be constructed as completely synthetic DNAs. Methods of efficiently synthesizing oligonucleotides in the range of 20 to about 150 nucleotides are widely available. See Ausubel et al , supra, section 2.11, Supplement 21 (1993). Overlapping oligonucleotides may be synthesized and assembled in a fashion first reported by Khorana et al, J. Mol. Biol. 72:209-217 (1971); see also Ausubel et al, Section 8.2. The synthetic DNAs are designed with convenient restriction sites engineered at the 5' and 3 ' ends of the gene to facilitate cloning into an appropriate vector. An alternative method of generating variants is to start with one of the inventive DNAs and then to conduct site-directed mutagenesis. See Ausubel et al , supra, chapter 8, Supplement 37 (1997). In a typical method, a target DNA is cloned into a single-stranded DNA bacteriophage vehicle. Single-stranded DNA is isolated and hybridized with a oligonucleotide containing the desired nucleotide alteration(s). The complementary strand is synthesized and the double stranded phage is introduced into a host. Some of the resulting progeny will contain the desired mutant, which can be confirmed using DNA sequencing. In addition, various methods are available that increase the probability that the progeny phage will be the desired mutant. These methods are well known to those in the field and kits are commercially available for generating such mutants. ISOLATING HOMOLOGS Methods By using the sequences disclosed herein as probes or as primers, and techniques such as PCR cloning and colony /plaque hybridization, one skilled in the art can obtain homologs. "Homologs" are essentially naturally-occurring variants and include allelic, species-specific and tissue-specific variants. Region-specific primers or probes derived from the nucleotide sequence(s) provided can be used to prime DNA synthesis and PCR amplification, as well as to identify colonies containing cloned DNA encoding a homolog using known methods (Innis et al, PCR Protocols, Academic Press, San Diego, CA (1990)). Such an application is useful in diagnostic methods, as described in more detail below, as well as in preparing full-length DNAs from various sources. The PCR primers are preferably at least 15 bases, and more preferably at least 18 bases in length. When selecting a primer sequence, it is preferred that the primer pairs have approximately the same G/C ratio, so that melting temperatures are approximately the same. As a general guide, the formula 3(G+C) + 2(A+T) = °C, is useful. When using primers derived from the inventive sequences, one skilled in the art will recognize that by employing high stringency conditions (e.g. , annealing at 50-60°C), only sequences with greater than 75% sequence identity to the primer will be amplified. By employing lower stringency conditions (e.g., annealing at 35-37°C), sequences which have greater than 40-50% sequence identity to the primer also will be amplified. The PCR product may be subcloned and sequenced to confirm that it indeed displays the expected sequence identity. The PCR fragment may then be used to isolate a full length cDNA clone by a variety of methods. For example, the amplified fragment may be labeled and used to screen a bacteriophage cDNA library. Alternatively, the labeled fragment may be used to screen a genomic library. PCR technology may also be utilized to isolate full length cDNA sequences. For example, RNA may be isolated, following standard procedures, from an appropriate cellular or tissue source. A reverse transcription reaction may be performed on the RNA using an oligonucleotide primer specific for the most 5 ' end of the amplified fragment for the priming of first strand synthesis. The resulting RNA/DNA hybrid may then be "tailed" with guanines using a standard terminal transferase reaction, the hybrid may be digested with RNAase H, and second strand synthesis may then be primed with a poly-C primer. Thus, cDNA sequences upstream of the amplified fragment may easily be isolated. For a review of cloning strategies which may be used, see e.g. , Sambrook et al. , 1989, supra. When using DNA probes derived from the inventive sequences for colony/plaque hybridization, one skilled in the art will recognize that by employing medium to high stringency conditions (e.g., hybridizing at 50-65°C in 5X SSPC and 50% formamide, and washing at 50-65°C in 0.5X SSPC), sequences having regions with greater than 90% sequence identity to the probe can be obtained, and that by employing lower stringency conditions (e.g., hybridizing at 35-37°C in 5X SSPC and 40-45% formamide, and washing at 42°C in SSPC), sequences having regions with greater than 35-45% sequence identity to the probe will be obtained. Suitably, genomic or cDNA libraries can be constructed and screened in accord with the previous paragraph. The libraries should be derived from a tissue or organism that is known to express the gene of interest, or that is suspected of expressing the gene. The clone containing the homolog may then be purified through methods routinely practiced in the art, and subjected to sequence analysis. Additionally, an expression library can be constructed utilizing DNA isolated from or cDNA synthesized from a tissue or organism that is known to express the gene of interest, or that is suspected of expressing the gene. In this manner, clones may be induced and screened using standard antibody screening techniques in conjunction with antibodies raised against the normal gene product, as described herein. (For screening techniques, see, for example, Harlow, E. and Lane, eds., 1988, ANTIBODIES: A LABORATORY MANUAL, Cold Spring Harbor Press, Cold Spring Harbor Press.) Human Homologs Any organism or tissue can be used as the source for homologs of the present invention so long as the organism or tissue naturally expresses such a protein or contains genes encoding the same. The most preferred organism for isolating homologs is human. PROTEINS OF THE INVENTION One class of proteins included within the invention is encoded by the inventive DNA molecules presented. Other proteins according to the invention are those encoded by the DNA variants described above. As noted, these variants are designed with the encoded proteins in mind. A preferred class of protein fragments includes those fragments which retain any biological activity. These molecules share functional features common the family of proteins, although these characteristics may vary in degree. According to one aspect of the invention fragments of the inventive proteins are contemplated. Some preferred fragments are those which are capable of eliciting an immune response. Generally these "antigenic" fragments will be from about five amino acids in length to about fifty amino acids in length. Some preferred antigenic fragments are from five to about twenty amino acids long. "Antigenic" response may refer to a T cell response, a B cell response or a response by cells of the macrophage/monocyte lineages. In most cases, however, it will refer to the immune response involved in the generation of antibodies. In other words, the relevant immune response is that of helper T cells and/or B cells. These preferred molecules comprise one or more T cell and /or B cell epitopes. ANTIBODIES OF THE INVENTION Antibodies raised against the proteins and protein fragments of the invention also are contemplated by the invention. Described below are antibody products and methods for producing antibodies capable of specifically recognizing one or more epitopes of the presently described proteins and their derivatives. Antibodies include, but are not limited to polyclonal antibodies, monoclonal antibodies (mAbs), humanized or chimeric antibodies, single chain antibodies including single chain Fv (scFv) fragments, Fab fragments, F(ab') 2 fragments, fragments produced by a Fab expression library, anti-idiotypic (anti-Id) antibodies, epitope-binding fragments, and humanized forms of any of the above. As known to one in the art, these antibodies may be used, for example, in the detection of a target protein in a biological sample. They also may be utilized as part of treatment methods, and/or may be used as part of diagnostic techniques whereby patients may be tested for abnormal levels or for the presence of abnormal forms of the such proteins. In general, techniques for preparing polyclonal and monoclonal antibodies as well as hybridomas capable of producing the desired antibody are well known in the art (Campbell, A.M., Monoclonal Antibody Technology: Laboratory Techniques in Biochemistry and Molecular Biology, Elsevier Science Publishers, Amsterdam, The Netherlands (1984); St. Groth et al., /. Immunol. Methods 35:1-21 (1980); Kohler and Milstein, Nature 256:495-497 (1975)), the trioma technique, the human B-cell hybridoma technique (Kozbor et al, Immunology Today 4:72 (1983); Cole et al, in Monoclonal Antibodies and Cancer Therapy, Alan R. Liss, Inc. (1985), pp. 77-96). Antibodies may also be generated by the known techniques of phage display and in vitro immunization. Polyclonal Antibodies Polyclonal antibodies are heterogeneous populations of antibody molecules derived from the sera of animals immunized with an antigen, such as an inventive protein or an antigenic derivative thereof. Polyclonal antiserum, containing antibodies to heterogeneous epitopes of a single protein, can be prepared by immunizing suitable animals with the expressed protein described above, which can be unmodified or modified, as known in the art, to enhance immunogenicity. Immunization methods include subcutaneous or intraperitoneal injection of the polypeptide. Effective polyclonal antibody production is affected by many factors related both to the antigen and to the host species. For example, small molecules tend to be less immunogenic than other and may require the use of carriers and/or adjuvant. In addition, host animal response may vary with site of inoculation. Both inadequate or excessive doses of antigen may result in low titer antisera. In general, however, small doses (high ng to low μg levels) of antigen administered at multiple intradermal sites appears to be most reliable. Host animals may include but are not limited to rabbits, mice, chickens and rats, to name but a few. An effective immunization protocol for rabbits can be found in Vaitukaitis, J. et αl, J. Clin. Endocrinol. Metαb. 33:988-991 (1971). The protein immunogen may be modified or administered in an adjuvant in order to increase the protein's antigenicity. Methods of increasing the antigenicity of a protein are well known in the art and include, but are not limited to coupling the antigen with a heterologous protein (such as globulin β-galactosidase) or through the inclusion of an adjuvant during immunization. Adjuvants include Freund's (complete and incomplete), mineral gels such as aluminum hydroxide, surface active substances such as lysolecithin, pluronic polyols, polyanions, peptides, oil emulsions, keyhole limpet hemocyanin, dimtrophenol, and potentially useful human adjuvants such as BCG (bacille Calmette-Guerin) and Corynebacteriumparvum. Booster injections can be given at regular intervals, with at least one usually being required for optimal antibody production. The antiserum may be harvested when the antibody titer begins to fall. Titer may be determined semi-quantitatively, for example, by double immunodiffusion in agar against known concentrations of the antigen. See, for example, Ouchterlony et al, Chap. 19 in: Handbook of Experimental Immunology, Wier, ed, Blackwell (1973). Plateau concentration of antibody is usually in the range of 0.1 to 0.2 mg/ml of serum (about 12 μM). The antiserum may be purified by affinity chromatography using the immobilized immunogen carried on a solid support. Such methods of affinity chromatography are well known in the art. Affinity of the antisera for the antigen may be determined by preparing competitive binding curves, as described, for example, by Fisher, Chap. 42 in: Manual of Clinical Immunology, second edition, Rose and Friedman, eds., Amer. Soc. For Microbiology, Washington, D.C. (1980). In addition to using protein an the immunogen, DNA molecules may be used directly. In this manner, a DNA encoding the protein immunogen is administered. Boosting and harvesting is done in a manner analogous to that detailed above. Yet another method of producing antibodies entails immunizing chickens and harvesting the antibodies from their eggs. Monoclonal Antibodies Monoclonal antibodies (MAbs), are homogeneous populations of antibodies to a particular antigen. They may be obtained by any technique which provides for the production of antibody molecules by continuous cell lines in culture or in vivo. MAbs may be produced by making hybridomas which are immortalized cells capable of secreting a specific monoclonal antibody. Monoclonal antibodies to any of the proteins, peptides and epitopes thereof described herein can be prepared from murine hybridomas according to the classical method of Kohler, G. and Milstein, C, Nature 256:495-497 (1975) (and U.S. Patent No. 4,376,110) or modifications of the methods thereof, such as the human B-cell hybridoma technique (Kosbor et al , 1983, Immunology Today 4:72; Cole et al , 1983, Proc. Natl. Acad. Sci. USA 80: 2026-2030), and the EBV-hybridoma technique (Cole et al , 1985, MONOCLONAL ANTIBODIES AND CANCER THERAPY, Alan R. Liss, Inc., pp. 77-96). In one method a mouse is repetitively inoculated with a few micrograms of the selected protein over a period of a few weeks. The mouse is then sacrificed, and the antibody producing cells of the spleen are isolated. The spleen cells are fused, typically using polyethylene glycol, with mouse myeloma cells, such as SP2/0-Agl4 myeloma cells. The excess, unfused cells are destroyed by growth of the system on selective media comprising aminopterin (HAT media). The successfully fused cells are diluted, and aliquots are plated to microliter plates where growth is continued. Antibody-producing clones (hybridomas) are identified by detection of antibody in the supernatant fluid of the wells by immunoassay procedures. These include ELISA, as originally described by Engvall, Meth. Enzymol. 70:419 (1980), western blot analysis, radioimmunoassay (Lutz et al , Exp. Cell Res. 175:109-124 (1988)) and modified methods thereof. Selected positive clones can be expanded and their monoclonal antibody product harvested for use. Detailed procedures for monoclonal antibody production are described in Davis, L. et al BASIC METHODS IN MOLECULAR BIOLOGY, Elsevier, New York. Section 21-2 (1989). The hybridoma clones may be cultivated w vitro or in vivo, for instance as ascites. Production of high titers of mAbs in vivo makes this the presently preferred method of production. Alternatively, hybridoma culture in hollow fiber bioreactors provides a continuous high yield source of monoclonal antibodies. The antibody class and subclass may be determined using procedures known in the art (Campbell, A.M. , Monoclonal Antibody Technology: Laboratory Techniques in Biochemistry and Molecular Biology, Elsevier Science Publishers, Amsterdam, The Netherlands (1984)). MAbs may be of any immunoglobulin class including IgG, IgM, IgE, IgA, IgD and any subclass thereof. Methods of purifying monoclonal antibodies are well known in the art. Antibody Derivatives and Fragments Fragments or derivatives of antibodies include any portion of the antibody which is capable of binding the target antigen, or a specific portion thereof. Antibody derivatives include poly-specific (e.g., bi-specific) antibodies, which contain binding sites specific for two or more different epitopes. These epitopes may be from the same or different inventive molecules or one or more epitope may be from a molecule not specifically disclosed here. Antibody fragments specifically include F(ab Fab, Fab' and Fv fragments. These can be generated from any class of antibody, but typically are made from IgG or IgM. They may be made by conventional recombinant DNA techniques or, using the classical method, by proteolytic digestion with papain or pepsin. See CURRENT PROTOCOLS IN IMMUNOLOGY, chapter 2, Coligan et α/. , eds., (John Wiley & Sons 1991-92). F(ab') 2 fragments are typically about 110 kDa (IgG) or about 150 kDa (IgM) and contain two antigen-binding regions, joined at the hinge by disulfide bond(s). Virtually all, if not all, of the Fc is absent in these fragments. Fab' fragments are typically about 55 kDa (IgG) or about 75 kDa (IgM) and can be formed, for example, by reducing the disulfide bond(s) of an F(ab') 2 fragment. The resulting free sulfhydryl group(s) may be used to conveniently conjugate Fab' fragments to other molecules, such as detection reagents (e.g. , enzymes). Fab fragments are monovalent and usually are about 50 kDa (from any source). Fab fragments include the light (L) and heavy (H) chain, variable (V L and V H , respectively) and constant (C L C H , respectively) regions of the antigen-binding portion of the antibody. The H and L portions are linked by an intramolecular disulfide bridge. Fv fragments are typically about 25 kDa (regardless of source) and contain the variable regions of both the light and heavy chains (V L and V H , respectively). Usually, the V L and V H chains are held together only by non-covalent interacts and, thus, they readily dissociate. They do, however, have the advantage of small size and they retain the same binding properties of the larger Fab fragments. Accordingly, methods have been developed to crosslink the V L and V H chains, using, for example, glutaraldehyde (or other chemical crosslinkers), intermolecular disulfide bonds (by incorporation of cysteines) and peptide linkers. The resulting Fv is now a single chain (i.e. , SCFv). Other antibody derivatives include single chain antibodies (U.S. Patent 4,946,778; Bird, Science 242:423-426 (1988); Huston et al , Proc. Natl. Acad. Sci. USA 85:5879-5883 (1988); and Ward et al. , Nature 334:544-546 (1989)). Single chain antibodies are formed by linking the heavy and light chain fragments of the Fv region via an amino acid bridge, resulting in a single chain FV (SCFv). One preferred method involves the generation of scFvs by recombinant methods, which allows the generation of Fvs with new specificities by mixing and matching variable chains from different antibody sources. In a typical method, a recombinant vector would be provided which comprises the appropriate regulatory elements driving expression of a cassette region. The cassette region would contain a DNA encoding a peptide linker, with convenient sites at both the 5' and 3' ends of the linker for generating fusion proteins. The DNA encoding a variable region(s) of interest may be cloned in the vector to form fusion proteins with the linker, thus generating an scFv. In an exemplary alternative approach, DNAs encoding two Fvs may be ligated to the DNA encoding the linker, and the resulting tripartite fusion may be ligated directly into a conventional expression vector. The scFv DNAs generated any of these methods may be expressed in prokaryotic or eukaryotic cells, depending on the vector chosen. Antibody fragments which recognize specific epitopes may be generated by known techniques. For example, such fragments include but are not limited to: the F(ab'^ fragments which can be produced by pepsin digestion of the antibody molecule and the Fab fragments which can be generated by reducing the disulfide bridges of the F(ab^ fragments. Alternatively, Fab expression libraries may be constructed (Huse et al., 1989, Science, 246: 1275-1281) to allow rapid and easy identification of monoclonal Fab fragments with the desired specificity. Derivatives also include "chimeric antibodies" (Morrison et al , Proc. Natl. Acad. Sci. , 81:6851-6855 (1984); Neuberger et al , Nature, 312:604-608 (1984); Takeda et al , Nature, 314:452-454 (1985)). These chimeras are made by splicing the DNA encoding a mouse antibody molecule of appropriate specificity with, for instance, DNA encoding a human antibody molecule of appropriate specificity. Thus, a chimeric antibody is a molecule in which different portions are derived from different animal species, such as those having a variable region derived from a murine mAb and a human immunoglobulin constant region. These are also known sometimes as "humanized" antibodies and they offer the added advantage of at least partial shielding from the human immune system. They are, therefore, particularly useful in therapeutic in vivo applications. Labeled Antibodies The present invention further provides the above-described antibodies in detectably labeled form. Antibodies can be detectably labelled through the use of radio isotopes, affinity labels (such as biotin, avidin, etc.), enzymatic labels (such as horseradish peroxidase, alkaline phosphatase, etc.) fluorescent labels (such as FITC or rhodamine, etc.), paramagnetic atoms, etc. Procedures for accomplishing such labeling are well-known in the art, for example see (Sternberger et al , J. Histochem. Cytochem. 18:315 (1970); Bayer et al, Meth. Enzym. 62:308 (1979); Engval et al, Immunol. 109:129 (1972); Goding, J. Immunol. Meth. 13:215 (1976)). The labeled antibodies of the present invention can be used for vitro, in vivo, and in situ diagnostic assays. Immobilized Antibodies The foregoing antibodies also may be immobilized on a solid support. Examples of such solid supports include plastics such as polycarbonate, complex carbohydrates such as agarose and sepharose, acrylic resins and such as polyacrylamide and latex beads. Techniques for coupling antibodies to such solid supports are well known in the art (Weiret al, "Handbook of Experimental Immunology" 4th Ed., Blackwell Scientific Publications, Oxford, England, Chapter 10 (1986); Jacoby et al, Meth. Enzym. 34 Academic Press, N.Y. (1974)). The immobilized antibodies of the present invention can be used for in vitro, in vivo, and in situ assays as well as for immunoaffimty purification of the proteins of the present invention. THERAPEUTIC AND DIAGNOSTIC COMPOSITIONS The proteins, antibodies and polynucleotides of the present invention can be formulated according to known methods to prepare pharmaceutically useful compositions, whereby these materials, or their functional derivatives, are combined in admixture with a pharmaceutically acceptable carrier vehicle. Suitable vehicles and their formulation, inclusive of other human proteins, e.g., human serum albumin, are described, for example, in Remington's Pharmaceutical Sciences (16th ed., Osol, A., Ed., Mack, Easton PA (1980)). In order to form a pharmaceutically acceptable composition suitable for effective administration, such compositions will contain an effective amount of one or more of the agents of the present invention, together with a suitable amount of carrier vehicle. Pharmaceutical compositions for use in accordance with the present invention may be formulated in conventional manner using one or more physiologically acceptable carriers or excipients. Thus, the compounds and their physiologically acceptable salts and solvate may be formulated for administration by inhalation or insufflation (either through the mouth or the nose) or oral, buccal, parenteral or rectal administration. For oral administration, the pharmaceutical compositions may take the form of, for example, tablets or capsules prepared by conventional means with pharmaceutically acceptable excipients such as binding agents (e.g. , pregelatinised maize starch, polyvinylpyrrolidone or hydroxypropyl methylcellulose); fillers (e.g. , lactose, microcrystalline cellulose or calcium hydrogen phosphate); lubricants (e.g. , magnesium stearate, talc or silica); disintegrants (e.g. , potato starch or sodium starch glycolate); or wetting agents (e.g. , sodium lauryl sulphate). The tablets may be coated by methods well known in the art. Liquid preparations for oral administration may take the form of, for example, solutions, syrups or suspensions, or they maybe presented as a dry product for constitution with water or other suitable vehicle before use. Such liquid preparations may be prepared by conventional means with pharmaceutically acceptable additives such as suspending agents (e.g. , sorbitol syrup, cellulose derivatives or hydrogenated edible fats); emulsifying agents (e.g. , lecithin or acacia); non-aqueous vehicles (e.g. , almond oil, oily esters, ethyl alcohol or fractionated vegetable oils); and preservatives (e.g. , methyl or propyl- p-hydroxybenzoates or sorbic acid). The preparations may also contain buffer salts, flavoring, coloring and sweetening agents as appropriate. Preparations for oral administration may be suitably formulated to give controlled release of the active compound. For buccal administration the composition may take the form of tablets or lozenges formulated in conventional manner. For administration by inhalation, the compounds for use according to the present invention are conveniently delivered in the form of an aerosol spray presentation from pressurized packs or a nebuliser, with the use of a suitable propellant, e.g. , dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas. In the case of a pressurized aerosol the dosage unit may be determined by providing a valve to deliver a metered amount. Capsules and cartridges of, e.g. gelatin for use in an inhaler or insufflator may be formulated containing a powder mix of the compound and a suitable powder base such as lactose or starch. The compounds may be formulated for parenteral administration by injection, e.g. , by bolus injection or continuous infusion. Formulations for injection may be presented in unit dosage form, e.g. , in ampules or in multi-dose containers, with an added preservative. The compositions may take such forms as suspensions, solutions or emulsions in oily or aqueous vehicles, and may contain formulatory agents such as suspending, stabilizing and/or dispersing agents. Alternatively, the active ingredient may be in powder form for constitution with a suitable vehicle, e.g. , sterile pyrogen-free water, before use. The compounds may also be formulated in rectal compositions such as suppositories or retention enemas, e.g. , containing conventional suppository bases such as cocoa butter or other glycerides. In addition to the formulations described previously, the compounds may also be formulated as a depot preparation. Such long acting formulations may be administered by implantation (for example subcutaneously or intramuscularly) or by intramuscular injection. Thus, for example, the compounds may be formulated with suitable polymeric or hydrophobic materials (for example as an emulsion in an acceptable oil) or ion exchange resins, or as sparingly soluble derivatives, for example, as a sparingly soluble salt. The compositions may, if desired, be presented in a pack or dispenser device which may contain one or more unit dosage forms containing the active ingredient. The pack may for example comprise metal or plastic foil, such as a blister pack. The pack or dispenser device may be accompanied by instructions for administration. RECOMBINANT CONSTRUCTS AND EXPRESSION The present invention further provides recombinant DNA constructs comprising one or more of the nucleotide sequences of the present invention. The recombinant constructs of the present invention comprise a vector, such as a plasmid or viral vector, into which a DNA or DNA fragment, typically bearing an open reading frame, is inserted, in either orientation. The gene products encoded by the subject DNAs may be produced by recombinant DNA technology using techniques well known in the art. See, for example, the techniques described in Sambrook et al., 1989, supra, and Ausubel et al., 1989, supra. Alternatively, the DNA sequences may be chemically synthesized using, for example, synthesizers. See, for example, the techniques described in OLIGONUCLEOTIDE SYNTHESIS, 1984, Gait, ed., IRL Press, Oxford, which is incorporated by reference herein in its entirety. They may be assembled from fragments and short oligonucleotide linkers, or from a series of oligonucleotides. The are preferably made by RT-PCR methods. The resulting synthetic gene is capable of being expressed in a recombinant vector. In some cases the recombinant constructs will be expression vectors, which are capable of expressing the RNA and/or protein products of the encoded DNA(s). Thus, the vector may further comprise regulatory sequences, including for example, a promoter, operably linked to the open reading frame (ORF). The vector may further comprise a selectable marker sequence. Specific initiation signals may also be required for efficient translation of inserted target gene coding sequences. These signals include the ATG initiation codon and adjacent sequences. In cases where a target DNA includes its own initiation codon and adjacent sequences is inserted into the appropriate expression vector, no additional translation control signals may be needed. However, in cases where only a portion of an ORF is used, exogenous translational control signals, including, perhaps, the ATG initiation codon, must be provided. Furthermore, the initiation codon must be in phase with the reading frame of the desired coding sequence to ensure translation of the entire target. These exogenous translational control signals and initiation codons can be of a variety of origins, both natural and synthetic. The efficiency of expression may be enhanced by the inclusion of appropriate transcription enhancer elements, transcription terminators, etc. (see Bittner et al, Methods in Enzymol. 153:516-544 (1987)). Some appropriate cloning and expression vectors for use with prokaryotic and eukaryotic hosts are described by Sambrook, et al, in Molecular Cloning: A Laboratory Manual, Second Edition, Cold Spring Harbor, New York (1989), the disclosure of which is hereby incorporated by reference. If desired, to enhance expression and facilitate proper protein folding, the codon context and codon pairing of the sequence may be optimized for the particular expression organism, as explained by Hatfieldet /., U.S. Patent No. 5,082,767. The present invention further provides host cells containing at least one of the DNAs of the present invention. The host cell can be virtually any cell for which expression vectors are available. It may be, for example, a higher eukaryotic host cell, such as a mammalian cell, a lower eukaryotic host cell, such as a yeast cell, or the host cell can be a prokaryotic cell, such as a bacterial cell. Introduction of the recombinant construct into the host cell can be effected by calcium phosphate transfection, DEAE, dextran mediated transfection, or electroporation (Davis et al, Basic Methods in Molecular Biology (1986)). A wide variety of expression systems are available, such as: yeast (e.g. Saccharomyces, Pichia) transformed with recombinant yeast expression vectors containing the target DNA; insect cell systems infected with recombinant virus expression vectors (e.g. , baculovirus) containing the target DNA sequences; plant cell systems infected with recombinant virus expression vectors (e.g. , cauliflower mosaic virus, CaMV; tobacco mosaic virus, TMV) or transformed with recombinant plasmid expression vectors (e.g. Ti plasmid) containing target DNA coding sequences; or mammalian cell systems (e.g. COS, CHO, BHK, 293, 3T3) harboring recombinant expression constructs containing promoters derived from the genome of mammalian cells (e.g. , metallothionein promoter) or from mammalian viruses (e.g. , the adenovirus late promoter; the vaccinia virus 7.5K promoter). Depending on the system chosen, the resulting product may differ. For example, proteins expressed in most bacterial cultures, e.g. , E. coli, will be free of glycosylation modifications; polypeptides or proteins expressed in yeast will have a glycosylation pattern different from that expressed in mammalian cells. Vectors Generally, recombinant expression vectors will include origins of replication and selectable markers permitting selection of the host cell, e.g. , the ampicillin resistance gene of E. coli and S. cerevisiae TRP1 gene, and a promoter derived from a highly -expressed gene to direct transcription of a downstream structural sequence. Such promoters can be derived from operons encoding glycolytic enzymes such as 3 -phosphogly cerate kinase (PGK), α-factor, acid phosphatase, or heat shock proteins, among others. The heterologous structural sequence is assembled in appropriate phase with translation initiation and termination sequence, and in one aspect of the invention, a leader sequence capable of directing secretion of translated protein into the periplasmic space or extracellular medium. Optionally, the heterologous sequence can encode a fusion protein including an N-terminal or C-terminal identification peptide imparting desired characteristics, e.g. , stabilization or simplified purification of expressed recombinant product. Bacterial Expression Useful expression vectors for bacterial use are constructed by inserting a structural DNA sequence encoding a desired protein together with suitable translation initiation and termination signals in operable reading phase with a functional promoter. The vector will comprise one or more phenotypic selectable markers and an origin of replication to ensure maintenance of the vector and, if desirable, to provide amplification within the host. Suitable prokaryotic hosts for transformation include E. coli, Bacillus subtilis, Salmonella typhimurium and various species within the genera Pseudomonas, Streptomyces, and Staphylococcus, although others may, also be employed as a matter of choice. Bacterial vectors may be, for example, bacteriophage-, plasmid- or cosmid-based. These vectors can comprise a selectable marker and bacterial origin of replication derived from commercially available plasmids typically containing elements of the well known cloning vector pBR322 (ATCC 37017). Such commercial vectors include, for example, GEM 1 (Promega Biotec, Madison, WI, USA), pBs, phagescript, PsiX174, pBluescript SK, pBs KS, pNH8a, pNHlόa, pNH18a, pNH46a (Stratagene); pTrc99A, pKK223-3, pKK233-3, pKK232-8, pDR540, and pRIT5 (Pharmacia). These "backbone" sections are combined with an appropriate promoter and the structural sequence to be expressed. Bacterial promoters include lac, T3, T7, lambda P R or P L , tip, and ara. Following transformation of a suitable host strain and growth of the host strain to an appropriate cell density, the selected promoter is derepressed/ induced by appropriate means (e.g., temperature shift or chemical induction) and cells are cultured for an additional period. Cells are typically harvested by centrifugation, disrupted by physical or chemical means, and the resulting crude extract retained for further purification. In bacterial systems, a number of expression vectors may be advantageously selected depending upon the use intended for the protein being expressed. For example, when a large quantity of such a protein is to be produced, for the generation of antibodies or to screen peptide libraries, for example, vectors which direct the expression of high levels of fusion protein products that are readily purified may be desirable. Such vectors include, but are not limited, to the E. coli expression vector pUR278 (Ruther et al., 1983, EMBO J. 2:1791), in which the coding sequence may be ligated into the vector in frame with the lac Z coding region so that a fiision protein is produced; pIN vectors (Inouye et al 1985, Nucleic Acids Res. 13:3101-3109; Van Heeke et α/. , 1989, 7. Biol Chem. 264:5503-5509); pET vectors, Studier et al , Methods in Enzymology 185: 60-89 (Academic Press 1990); and the like. Moreover, pGEX vectors may be used to express foreign polypeptides as fusion proteins with glutathione S-transferase (GST). In general, such fusion proteins are soluble and easily can be purified from lysed cells by adsorption to glutathione-agarose beads followed by elution in the presence of free glutathione. The pGEX vectors are designed to include thrombin or factor Xa protease cleavage sites so that the cloned target gene protein can be released from the GST moiety. In a one embodiment, full length cDNA sequences are appended with in-frame BamHl sites at the amino terminus and EcoRI sites at the carboxyl terminus using standard PCR methodologies (Innis et al., 1990, supra) and ligated into the pGEX-2TK vector (Pharmacia, Uppsala, Sweden). The resulting cDNA construct contains a kinase recognition site at the amino terminus for radioactive labeling and glutathione S-transferase sequences at the carboxyl terminus for affinity purification (Nilsson, et al. 1985, EMBO J. 4: 1075; Zabeau and Stanley, 1982, EMBO J. 1: 1217. Eukaryotic Expression Various mammalian cell culture systems can also be employed to express recombinant protein. Examples of mammalian expression systems include the COS-7 lines of monkey kidney fibroblasts, described by Gluzman, Cell 23:175 (1981), and other cell lines capable of expressing a compatible vector, for example, the C127, 3T3, CHO, HeLa and BHK cell lines. Mammalian expression vectors will comprise an origin of replication, a suitable promoter and enhancer, and also any necessary ribosome binding sites, polyadenylation site, splice donor and acceptor sites, transcriptional termination sequences, and 5' flanking nontranscribed sequences. DNA sequences derived from the SV40 viral genome, for example, SV40 origin, early promoter, enhancer, splice, and polyadenylation sites may be used to provide the required nontranscribed genetic elements. Mammalian promoters include CMV immediate early, HSV thymidine kinase, early and late SV40, LTRs from retrovirus, and mouse metallothionein-I. Exemplary mammalian vectors include pWLneo, pSV2cat, pOG44, pXTl, pSG (Stratagene) pSVK3, pBPV, pMSG, and pSVL (Pharmacia). Selectable markers include CAT (chloramphenicol transferase). In mammalian host cells, a number of viral-based expression systems may be utilized. In cases where an adenovirus is used as an expression vector, the coding sequence of interest may be ligated to an adenovirus transcription/translation control complex, e.g. , the late promoter and tripartite leader sequence. This chimeric gene may then be inserted in the adenovirus genome by in vitro or in vivo recombination. Insertion in a non-essential region of the viral genome (e.g. , region El or E3) will result in a recombinant virus that is viable and capable of expressing a target protein in infected hosts. (E.g. , See Logan et al. , 1984, Proc. Natl Acad. Sci. USA 81:3655-3659). In one embodiment, cDNA sequences encoding the full-length open reading frames are ligated into pCMVβ replacing the β-galactosidase gene such that cDNA expression is driven by the CMV promoter (Alam, 1990, Anal. Biochem. 188: 245-254; MacGregor et al , 1989, Nucl Acids Res. 17: 2365; Norton et al 1985, Mol. Cell Biol. 5: 281). In addition, a host cell strain may be chosen which modulates the expression of the inserted sequences, or modifies and processes the gene product in the specific fashion desired. Such modifications (e.g. , glycosylation) and processing (e.g. , cleavage) of protein products may be important for the function of the protein. Different host cells have characteristic and specific mechanisms for the post-translational processing and modification of proteins. Appropriate cell lines or host systems can be chosen to ensure the correct modification and processing of the foreign protein expressed. To this end, eukaryotic host cells which possess the cellular machinery for proper processing of the primary transcript, glycosylation, and phosphorylation of the gene product may be used. Such mammalian host cells include but are not limited to CHO, VERO, BHK, HeLa, COS, MDCK, 293, 3T3, WI38, etc. For long-term, high-yield production of recombinant proteins in eukaryotic cells, stable expression is preferred. Rather than using expression vectors which contain viral origins of replication, host cells can be transformed with DNA controlled by appropriate expression control elements (e.g. , promoter, enhancer, sequences, transcription terminators, polyadenylation sites, etc.), and a selectable marker. Following the introduction of the foreign DNA, engineered cells may be allowed to grow for 1-2 days in an enriched media, and then are switched to a selective media. The selectable marker in the recombinant plasmid confers resistance to the selection and allows cells to stably integrate the plasmid into their chromosomes and grow to form foci which in turn can be cloned and expanded into cell lines. This method may advantageously be used to engineer cell lines which express the target protein. Such engineered cell lines may be particularly useful in screening and evaluation of compounds that affect the endogenous activity of the protein. A number of selection systems may be used, including but not limited to the herpes simplex virus thymidine kinase (Wigler, et al , Cell 11:223 (1977)), hypoxanthine-guanine phosphoribosyltransferase(Szybalskaet α/., Proc. Natl. Acad. Sci. USA 48:2026 (1962)), and adenine phosphoribosyltransferase(Lowy, et al , Cell 22:817 (1980)) genes can be employed in tk " , hgprt " or aprf cells, respectively. Also, antimetabolite resistance can be used as the basis of selection for dhfr, which confers resistance to methotrexate (Wigler, et al. , Proc. Natl. Acad, Sci. USA 77:3567 (1980)); O'Hare, et al , 1981, Proc. Natl. Acad. Sci. USA 78:1527); gpt, which confers resistance to mycophenolic acid (Mulligan et al , Proc. Natl. Acad. Sci. USA 78:2072 (1981)); neo, which confers resistance to the aminoglycoside G-418 (Colberre-Garapin, et al. , 1981, J. Mol. Biol 150: 1); and hydro, which confers resistance to hygromycin (Santerre, et al. , 1984, Gene 30: 147) genes. An alternative fusion protein system allows for the ready purificationof non-denatured fusion proteins expressed in human cell lines (Janknecht, et al. , Proc. Natl Acad. Sci. USA 88: 8972-8976 (1991)). In this system, the gene of interest is subcloned into a vaccinia-based plasmid such that the gene's open reading frame is translationally fused to an amino-terminal tag consisting of six histidine residues. Extracts from cells infected with recombinant vaccinia virus are loaded onto N? + nitriloacetic acid-agarose columns and histidine-tagged proteins are selectively eluted with imidazole-containing buffers. In an insect system, Autographa californica nuclear polyhedrosis virus (AcNPV) is used as a vector to express foreign genes. The virus grows in Spodoptera frugiperda cells. The target coding sequence may be cloned individually into non-essential regions (for example the polyhedrin gene) of the virus and placed under control of an AcNPV promoter (for example the polyhedrin promoter). Successful insertion of a target gene coding sequence will result in inactivation of the polyhedrin gene and production of non-occluded recombinant virus (i.e., virus lacking the proteinaceous coat coded for by the polyhedrin gene). These recombinant viruses are then used to infect Spodoptera frugiperda cells in which the inserted gene is expressed. (E.g. , see Smith et al., 1983, J. Virol. 46: 584; Smith, U.S. Patent No. 4,215,051). While the present proteins can be expressed in recombinant systems, as described above, cell-free translation systems can also be employed to produce such proteins using RNAs derived from the DNA constructs of the present invention. Purification of Recombinant Proteins Recombinant proteins produced may be isolated by host cell lysis. This may be followed by one or more salting-out, aqueous ion exchange or size exclusion chromatography steps. Finally, high performance liquid chromatography (HPLC) can be employed for final purification steps. Microbial cells employed in expression of proteins can be disrupted by any convenient method, including freeze-thaw cycling, sonication, mechanical disruption, or use of cell lysing agents, like lysozyme and chelators. If inclusion bodies are formed in bacterial systems, they may be extracted from cell pellets using, for example, detergents, reducing agents, salts, urea, guanidinium chloride and extremes of pH (e.g. <4 or > 10). If denaturation occurs, protein refolding steps (e.g. , dialysis) can be used, as necessary, in completing configuration of the mature protein. If disulfide bridges are present in the native protein, they may be reoxidized using known methods. By way of specific non-limiting example, the recombinant bacterial cells, for example E. coli, are grown in any of a number of suitable media, for example LB, and the expression of the recombinant protein induced by adding IPTG (e.g. , lac operator-promoter) to the media or switching incubation to a higher temperature (e.g. , λ cl 857 ). After culturing the bacteria for a further period of between 2 and 24 hours, the cells are collected by centrifugation and washed to remove residual media. The bacterial cells are then lysed, for example, by disruption in a cell homogenizer and centrifuged to separate the cell membranes from the soluble cell components. If the protein aggregates into inclusion bodies, this centrifugation can be performed under conditions whereby the dense inclusion bodies are selectively enriched by incorporation of sugars such as sucrose into the buffer and centrifugation at a selective speed. The inclusion bodies can then be washed in any of several solutions to remove some of the contaminating host proteins, then solubilized in solutions containing high concentrations of urea (e.g. 8M) or chaotropic agents such as guanidinium hydrochloride in the presence of reducing agents such as β-mercaptoethanolor DTT (dithiothreitol). At this stage it may be advantageous to incubate the protein for several hours under conditions suitable for the protein to undergo a refolding process into a conformation which more closely resembles that of the native protein. Such conditions generally include low protein concentrations less than 500 μg/ml), low levels of reducing agent, concentrations of urea less than 2 M and often the presence of reagents such as a mixmre of reduced and oxidized glutathione which facilitate the interchange of disulphide bonds within the protein molecule. The refolding process can be monitored, for example, by SDS-PAGE or with antibodies which are specific for the native molecule. Following refolding, the protein can then be purified further and separated from the refolding mixture by chromatography on any of several supports including ion exchange resins, gel permeation resins or on a variety of affinity columns. Labeling Proteins When used as a component in assay systems such as those described, below, the target protein may be labeled, either directly or indirectly, to facilitate detection of the present res- like molecules either in vitro or in vivo. Any of a variety of suitable labeling systems may be used including but not limited to radioisotopes such as 125 I; enzyme labeling systems that generate a detectable colorimetric signal or light when exposed to substrate; and fluorescent labels. Where recombinant DNA technology is used for protein production the, it may be advantageous to engineer fusion proteins that can facilitate labeling, immobilization and/or detection. These fusion proteins may, for example, add amino acids which facilitate further chemical modification. They also may add a functional moiety, such as an enzyme, which directly facilitates detection. TRANSGENIC ANIMALS The invention further contemplates animal models for studying the function of the present molecules and for overproducing the protein products. The disclosed DNA sequences may be used in conjunction with techniques for producing transgenic animals that are well known to those of skill in the art. To prepare transgenic animals, target gene sequences may for example be introduced into, and overexpressed in, the genome of the animal of interest, or, if endogenous target gene sequences are present, they may either be overexpressed or, alternatively, be disrupted in order to underexpress or inactivate target gene expression, such as described for the disruption of apoE in mice (Plum et al , Cell 71 : 343-353 (1992)). In order to overexpress a target gene sequence, the coding portion of the target gene sequence may be ligated to a regulatory sequence which is capable of driving gene expression in the animal and cell type of interest. Such regulatory regions will be well known to those of skill in the art, and may be utilized in the absence of undue experimentation. For underexpression of an endogenous target gene sequence, such a sequence may be isolated and engineered such that when reintroduced into the genome of the animal of interest, the endogenous target gene alleles will be inactivated. Preferably, the engineered target gene sequence is introduced via gene targeting such that the endogenous target sequence is disrupted upon integration of the engineered target gene sequence into the animal ' s genome . Animals of any species, including, but not limited to, mice, rats, rabbits, guinea pigs, pigs, micro-pigs, goats, and non-human primates, e.g. , baboons, monkeys, and chimpanzees may be used to generate cardiovascular disease animal models. Goats, cows and sheep are particularly preferred for producing protein in vivo. Any technique known in the art may be used to introduce a target gene transgene into animals to produce the founder lines of transgenic animals. Such techniques include, but are not limited to pronuclear microinjection (Hoppe et al , U.S. Pat. No. 4,873,191 (1989)); retrovirus mediated gene transfer into germ lines (Van der Putten et al. , Proc. Natl. Acad. Sci., USA 82:6148-6152 (1985)); gene targeting in embryonic stem cells (Thompson et al , Cell 56:313-321 (1989)); electroporation of embryos (Lo, Mol. Cell. Biol. 3:1803-1814 (1983)); and sperm-mediated gene transfer (Lavitrano et al , Cell 57:717-723 (1989)); etc. For a review of such techniques, see Gordon, Transgenic Animals, Intl. Rev. Cytol. 115:171- 229 (1989). The present invention provides for transgenic animals that carry the transgene in all their cells, as well as animals which carry the transgene in some, but not all their cells, i.e. , mosaic animals. The transgene may be integrated as a single transgene or in concatamers, e.g. , head-to-head tandems or head-to-tail tandems. The transgene may also be selectively introduced into and activated in a particular cell type by following, for example, the teaching of Lasko et al. (Lasko et al , Proc. Natl Acad. Sci. USA 89:3232-6236 (1992)). The regulatory sequences required for such a cell-type specific activation will depend upon the particular cell type of interest, and will be apparent to those of skill in the art. When it is desired that the target gene be integrated into the chromosomal site of the endogenous target gene, gene targeting is preferred. Briefly, when such a technique is to be utilized, vectors containing some nucleotide sequences homologous to the endogenous target gene of interest are designed for the purpose of integrating, via homologous recombination with chromosomal sequences, into and disrupting the function of the nucleotide sequence of the endogenous target gene. The transgene may also be selectively introduced into a particular cell type, thus inactivating the endogenous gene of interest in only that cell type, by following, for example, the teaching of Gu et al. Science 265: 103-106 (1994)). The regulatory sequences required for such a cell-type specific inactivation will depend upon the particular cell type of interest, and will be apparent to those of skill in the art. Once transgenic animals have been generated, the expression of the recombinant target gene and protein may be assayed utilizing standard techniques. Initial screening may be accomplished by Southern blot analysis or PCR techniques to analyze animal tissues to assay whether integration of the transgene has taken place. The level of mRNA expression of the transgene in the tissues of the transgenic animals may also be assessed using techniques which include but are not limited to Northern blot analysis of tissue samples obtained from the animal, in situ hybridization analysis, and RT-PCR. Samples of target gene-expressing tissue, may also be evaluated immunocytochemically using antibodies specific for the target gene transgene gene product of interest. The transgenic animals that express target gene mRNA or target gene transgene peptide (detected immunocytochemically, using antibodies directed against the target gene product's epitopes) at easily detectable levels should then be further evaluated to identify those animals which display characteristic increased susceptibility to carcinogenesis. Additionally, specific cell types within the transgenic animals may be analyzed and assayed in vitro for cellular phenotypes characteristic of mutant phenotype. Once target gene transgenic founder animals are produced, they may be bred, inbred, outbred, or crossbred to produce colonies of the particular animal. Examples of such breeding strategies include but are not limited to: outbreeding of founder animals with more than one integration site in order to establish separate lines; inbreeding of separate lines in order to produce compound target gene transgenics that express the target gene transgene of interest at higher levels because of the effects of additive expression of each target gene transgene; crossing of heterozygous transgenic animals to produce animals homozygous for a given integration site in order both to augment expression and eliminate the possible need for screening of animals by DNA analysis; crossing of separate homozygous lines to produce compound heterozygous or homozygous lines; breeding animals to different inbred genetic backgrounds so as to examine effects of modifying alleles on expression of the target gene transgene and the possible development of carcinogenesis. One such approach is to cross the target gene transgenic founder animals with a wild type strain to produce an Fl generation that exhibits increased susceptibility to carcinogenesis. The Fl generation may then be inbred in order to develop a homozygous line, if it is found that homozygous target gene transgenic animals are viable. Methods of generating "knockout" mice using homologous recombination in embryonic stem cells are well known in the art. Suitable methods are described, for example, in Mansour et al , Nature, 336:348 (1988); Zijlstra et al , Nature, 342:435 (1989) and 344:742 (1990); and Hasty et al , Nature, 350:243 (1991). This genomic DNA can be obtained by conventional methods using the cDNA sequence as a probe in a commercially- available genomic DNA library. Briefly, a genomic fragment is cleaved with a restriction endonuclease and a heterologous cassette containing a neomycin-resistancegene is inserted at the cleavage site. A suitable cassette is the GTI-II neo cassette described by Lufkin et al , Cell 66:1105 (1991). The modified genomic fragment is cloned into a suitable targeting vector that is introduced into murine embryonic stem cells by electroporation. Cells that have undergone homologous recombination (and hence disruption of the gene) are selected by resistance to G418, and used to generate chimeric mice using well known methods. See Lufkin et al, supra. Traditional breeding methods then can be used to generate mice that are homozygous for the disrupted gene. The phenotype of mice that are homozygous for the mutation then can be studied to provide insights into the role of the protein in, for example, carcinogenesis. These mice also can be used as models for developing new treatments for cancers. If this mutation is lethal in homozygous mice (for example during embryogenesis) heterozygous mice, which express only half the amount of the protein can also be studied. GENE THERAPY APPLICATIONS When mutations in the inventive protein, or in the elements controlling expression of that protein, are found to be associated with a malignant phenotype, control of cellular proliferation can be restored by gene therapy methods. For example, overexpression of the protein can be counteracted by concurrent expression of an antisense molecule that binds to and inhibits expression of the mRNA encoding the protein. Alternatively, overexpression can be inhibited in an analogous manner using a ribozyme that cleaves the mRNA. In another embodiment, where expression of a mutated protein induces the malignant phenotype, concomitant expression of the non-mutated molecule via introduction of an exogenous gene may be used. Methods of using antisense and ribozyme technology to control gene expression, or of gene therapy methods for expression of an exogenous gene in this manner are well known in the art. Each of these methods requires a system for introducing a vector into the cells containing the mutated gene. The vector encodes either an antisense or ribozyme transcript of the inventive protein. The construction of a suitable vector can be achieved by any of the methods well-known in the art for the insertion of exogenous DNA into a vector. See, e.g. , Sambrook et al, Molecular Cloning (Cold Spring Harbor Press 2d ed. 1989), which is incorporated herein by reference. In addition, the prior art teaches various methods of introducing exogenous genes into cells in vivo. See Rosenberg et al. , Science 242: 1575-1578 (1988) and Wolff et al , PNAS 86:9011-9014 (1989), which are incorporated herein by reference. The routes of delivery include systemic admimstration and admimstration in situ. Well-known techniques include systemic administration with cationic liposomes, and admimstration in situ with viral vectors. Any one of the gene delivery methodologies described in the prior art is suitable for the introduction of a recombinant vector containing an inventive gene according to the invention into a MTX-resistant, transport-deficient cancer cell. A listing of present-day vectors suitable for the purpose of this invention is set forth in Hodgson, Bio /Technology 13: 222 (1995), which is incorporated by reference. For example, liposome-mediated gene transfer is a suitable method for the introduction of a recombinant vector containing an inventive gene according to the invention into a MTX-resistant, transport-deficient cancer cell. The use of a cationic liposome, such as DC-Chol/DOPE liposome, has been widely documented as an appropriate vehicle to deliver DNA to a wide range of tissues through intravenous injection of DNA/cationic liposome complexes. See Caplen et al , Nature Med. 1:39-46 (1995) and Zhu et al, Science 261:209- 211 (1993), which are herein incorporated by reference. Liposomes transfer genes to the target cells by fusing with the plasma membrane. The entry process is relatively efficient, but once inside the cell, the liposome-DNA complex has no inherent mechanism to deliver the DNA to the nucleus. As such, the most of the lipid and DNA gets shunted to cytoplasmic waste systems and destroyed. The obvious advantage of liposomes as a gene therapy vector is that liposomes contain no proteins, which thus minimizes the potential of host immune responses. As another example, viral vector-mediated gene transfer is also a suitable method for the introduction of the vector into a target cell. Appropriate viral vectors include adenovirus vectors and adeno-associated virus vectors, retrovirus vectors and herpesvirus vectors. Adenoviruses are linear, double stranded DNA viruses complexed with core proteins and surrounded by capsid proteins. The common serotypes 2 and 5, which are not associated with any human malignancies, are typically the base vectors. By deleting parts of the virus genome and inserting the desired gene under the control of a constitutive viral promoter, the virus becomes a replication deficient vector capable of transferring the exogenous DNA to differentiated, non-proliferating cells. To enter cells, the adenovirus fibre interacts with specific receptors on the cell surface, and the adenovirus surface proteins interact with the cell surface integrins. The virus penton-cell integrin interaction provides the signal that brings the exogenous gene-containing virus into a cytoplasmic endosome. The adenovirus breaks out of the endosome and moves to the nucleus, the viral capsid falls apart, and the exogenous DNA enters the cell nucleus where it functions, in an epichromosomal fashion, to express the exogenous gene. Detailed discussions of the use of adenoviral vectors for gene therapy can be found in Berkner, Biotechniques 6:616-629 (1988) and Trapnell, Advanced Drug Delivery Rev. 12: 185-199 (1993), which are herein incorporated by reference. Adenovirus-derived vectors, particularly non-replicative adenovirus vectors, are characterized by their ability to accommodate exogenous DNA of 7.5 kB, relative stability, wide host range, low pathogenicity in man, and high titers (10 4 to 10 5 plaque forming units per cell). See Stratford- Perricaudet et al , PNAS 89:2581 (1992). Adeno-associated virus (AAV) vectors also can be used for the present invention. AAV is a linear single-stranded DNA parvovirus that is endogenous to many mammalian species. AAV has a broad host range despite the limitation that AAV is a defective parvovirus which is dependent totally on either adenovirus or herpesvirus for its reproduction in vivo. The use of AAV as a vector for the introduction into target cells of exogenous DNA is well-known in the art. See, e.g. , Lebkowski et al , Mole. & Cell. Biol 8:3988 (1988), which is incorporated herein by reference. In these vectors, the capsid gene of AAV is replaced by a desired DNA fragment, and transcomplementation of the deleted capsid function is used to create a recombinant virus stock. Upon infection the recombinant virus uncoats in the nucleus and integrates into the host genome. Another suitable virus-based gene delivery mechanism is retroviral vector-mediated gene transfer. In general, retroviral vectors are well-known in the art. See Breakfield et al. , Mole. Neuro. Biol 1:339 (1987) and Shih et al , in Vaccines 85: 177 (Cold Spring Harbor Press 1985). A variety of retroviral vectors and retroviral vector-producing cell lines can be used for the present invention. Appropriate retroviral vectors include Moloney Murine Leukemia Virus, spleen necrosis virus, and vectors derived from retroviruses such as Rous Sarcoma Virus, Harvey Sarcoma Virus, avian leukosis virus, human immunodeficiency virus, myeloproliferative sarcoma virus, and mammary tumor virus. These vectors include replication-competent and replication-defective retroviral vectors. In addition, amphotropic and xenotropic retroviral vectors can be used. In carrying out the invention, retroviral vectors can be introduced to a tumor directly or in the form of free retroviral vector producing-cell lines. Suitable producer cells include fibroblasts, neurons, glial cells, keratinocytes, hepatocytes, connective tissue cells, ependymal cells, chromaffin cells. See Wolff et al, PNAS 84:3344 (1989). Retroviral vectors generally are constructed such that the majority of its structural genes are deleted or replaced by exogenous DNA of interest, and such that the likelihood is reduced that viral proteins will be expressed. See Bender et al, J. Virol. 61: 1639 (1987) and Armento et al, J. Virol 61:1647 (1987), which are herein incorporated by reference. To facilitate expression of the antisense or ribozyme molecule, of the inventive protein, a retroviral vector employed in the present invention must integrate into the genome of the host cell genome, an event which occurs only in mitotically active cells. The necessity for host cell replication effectively limits retroviral gene expression to tumor cells, which are highly replicative, and to a few normal tissues. The normal tissue cells theoretically most likely to be transduced by a retroviral vector, therefore, are the endothelial cells that line the blood vessels that supply blood to the tumor. In addition, it is also possible that a retroviral vector would integrate into white blood cells both in the tumor or in the blood circulating through the tumor. The spread of retroviral vector to normal tissues, however, is limited. The local administration to a tumor of a retroviral vector or retroviral vector producing cells will restrict vector propagation to the local region of the tumor, minimizing transduction, integration, expression and subsequent cytotoxic effect on surrounding cells that are mitotically active. Both replicatively deficient and replicatively competent retroviral vectors can be used in the invention, subject to their respective advantages and disadvantages. For instance, for mmors that have spread regionally, such as lung cancers, the direct injection of cell lines that produce replication-deficient vectors may not deliver the vector to a large enough area to completely eradicate the tumor, since the vector will be released only form the original producer cells and their progeny, and diffusion is limited. Similar constraints apply to the application of replication deficient vectors to tumors that grow slowly, such as human breast cancers which typically have doubling times of 30 days versus the 24 hours common among human gliomas. The much shortened survival-time of the producer cells, probably no more than 7-14 days in the absence of immunosuppression, limits to only a portion of their replicative cycle the exposure of the tumor cells to the retroviral vector. The use of replication-defective retroviruses for treating mmors requires producer cells and is limited because each replication-defective retrovirus particle can enter only a single cell and cannot productively infect others thereafter. Because these replication- defective retroviruses cannot spread to other tumor cells, they would be unable to completely penetrate a deep, multilayered tumor in vivo. See Markert et al, Neurosurg. 77: 590 (1992). The injection of replication-competent retroviral vector particles or a cell line that produces a replication-competent retroviral vector virus may prove to be a more effective therapeutic because a replication competent retroviral vector will establish a productive infection that will transduce cells as long as it persists. Moreover, replicatively competent retroviral vectors may follow the tumor as it metastasizes, carried along and propagated by transduced tumor cells. The risks for complications are greater, with replicatively competent vectors, however. Such vectors may pose a greater risk then replicatively deficient vectors of transducing normal tissues, for instance. The risks of undesired vector propagation for each type of cancer and affected body area can be weighed against the advantages in the situation of replicatively competent verses replicatively deficient retroviral vector to determine an optimum treatment. Both amphotropic and xenotropic retroviral vectors may be used in the invention. Amphotropic viruses have a very broad host range that includes most or all mammalian cells, as is well known to the art. Xenotropic viruses can infect all mammalian cell cells except mouse cells. Thus, amphotropic and xenotropic retroviruses from many species, including cows, sheep, pigs, dogs, cats, rats, and mice, inter alia can be used to provide retroviral vectors in accordance with the invention, provided the vectors can transfer genes into proliferating human cells in vivo. Clinical trials employing retroviral vector therapy treatment of cancer have been approved in the United States. See Culver, Clin. Chem. 40: 510 (1994). Retroviral vector- containing cells have been implanted into brain tumors growing in human patients. See Oldfield et al, Hum. Gene Ther. 4: 39 (1993). These retroviral vectors carried the HSV-1 thymidine kinase (HSV-tk) gene into the surrounding brain tumor cells, which conferred sensitivity of the mmor cells to the antiviral drug ganciclovir. Some of the limitations of current retroviral based cancer therapy, as described by Oldfield are: (1) the low titer of virus produced, (2) virus spread is limited to the region surrounding the producer cell implant, (3) possible immune response to the producer cell line, (4) possible insertional mutagenesis and transformation of retroviral infected cells, (5) only a single treatment regimen of pro-drug, ganciclovir, is possible because the "suicide" product kills retrovirally infected cells and producer cells and (6) the bystander effect is limited to cells in direct contact with retrovirally transformed cells. See Bi et al. , Human Gene Therapy 4: 725 (1993). Yet another suitable virus-based gene delivery mechanism is herpesvirus vector- mediated gene transfer. While much less is known about the use of herpesvirus vectors, replication-competent HSV-1 viral vectors have been described in the context of antitumor therapy. See Martuza et al, Science 252: 854 (1991), which is incorporated herein by reference. DIAGNOSTIC METHODS The present invention also contemplates, for certain molecules described below, methods for diagnosis of human disease. In particular, patients can be screened for the occurrence of cancers, or likelihood of occurrence of cancers, associated with mutations in the encoded protein. DNA from tumor tissue obtained from patients suffering from cancer can be isolated and the gene encoding the protein can be sequenced. By examining a number of patients in this manner, mutations in the gene that are associated with a malignant cellular phenotype can be identified. In addition, correlation of the nature of the observed mutations with subsequent observed clinical outcomes allows development of prognostic model for the predicted outcome in a particular patient. Screening for mutations conveniently can be carried out at the DNA level by use of PCR, although the skilled artisan will be aware that many other well known methods are available for the screening. PCR primers can be selected that flank known mutation sites, and the PCR products can be sequenced to detect the occurrence of the mutation. Alternatively, the 3 ' residue of one PCR primer can be selected to be a match only for the residue found in the unmutated gene. If the gene is mutated, there will be a mismatch at the 3' end of the primer, and primer extension cannot occur, and no PCR product will be obtained. Alternatively, primer mixtures can be used where the 3' residue of one primer is any nucleotide other than the nonmutated residue. Observation of a PCR product then indicates that a mutation has occurred. Other methods of using, for example, oligonucleotide probes to screen for mutations are described, or example, in U.S. Patent No. 4,871,838, which is herein incorporated by reference in its entirety. Alternatively, antibodies can be generated that selectively bind either mutated or non- mutated protein. The antibodies then can be used to screen tissue samples for occurrence of mutations in a manner analogous to the DNA-based methods described supra. The diagnostic methods described above can be used not only for diagnosis and for prognosis of existing disease, but may also be used to predict the likelihood of the future occurrence of disease. For example, clinically healthy patients can be screened for mutations in the inventive molecule that correlate with later disease onset. Such mutations may be observed in the heterozygous state in healthy individuals. In such cases a single mutation event can effectively disable proper functioning of the gene and induce a transformed or malignant phenotype. This screening also may be carried out prenatally or neonatally. DNA molecules according to the invention also are well suited for use in so-called "gene chip" diagnostic applications. Such applications have been developed by, inter alia, Synteni and Affymetrix. Briefly, all or part of the DNA molecules of the invention can be used either as a probe to screen a polynucleotide array on a "gene chip," or they may be immobilized on the chip itself and used to identify other polynucleotides via hybridization to the surface of the chip. In this manner, for example, related genes can be identified, or expression patterns of the gene in various tissues can be simultaneously studied. Such gene chips have particular application for diagnosis of disease, or in forensic analysis to detect the presence or absence of an analyte. Suitable chip technology is described for example, in Wodicka et al , Nature Biotechnology, 15: 1359 (1997) which is hereby incorporated by reference in its entirety, and references cited therein. PROTEIN-PROTEIN INTERACTIONS Due to their similarity to certain known proteins, it is anticipated that some of the inventive protein molecules will interact with another class of cellular proteins. This is particularly true of those molecule containing leucine zipper motifs. Any method suitable for detecting protein-protein interactions can be employed for identifying interacting targets. Among the traditional methods which can be employed are co- immunoprecipitation, crosslinking and co-purification through gradients or chromatographic columns. Utilizing procedures such as these allows for the identification of GAP gene products. Once identified, a GAP protein can be used, in conjunction with standard techniques, to identify its corresponding pathway gene. For example, at least a portion of the amino acid sequence of the pathway gene product can be ascertained using techniques well known to those of skill in the art, such as via the Edman degradation technique (see, e.g.. Creighton, 1983, PROTEINS: STRUCTURES AND MOLECULAR PRINCIPLES, W.H. Freeman & Co. , N. Y. , pp.34-49). The amino acid sequence obtained can be used as a guide for the generation of oligonucleotide mixtures that can be used to screen for pathway gene sequences. Screening can be accomplished, for example, by standard hybridization or PCR techniques. Techniques for the generation of oligonucleotide mixtures and for screening are well-known. (See e.g. , Ausubel, supra, and PCR PROTOCOLS: A GUIDE TO METHODS AND APPLICATIONS, 1990, Innis et al. , eds. Academic Press, Inc. , New York). Additionally, methods can be employed which result in the simultaneous identification of interacting target genes. One method which detects protein interactions in vivo, the two- hybrid system, is described in detail for illustration purposes only and not by way of limitation. One version of this system has been described (Chien et al. , Proc. Natl Acad. Sci. USA, 88: 9578-9582 (1991)) and is commercially available from Clontech (Palo Alto, CA). Briefly, utilizing such a system, plasmids are constructed that encode two hybrid proteins: one consists of the DNA-binding domain of a transcription activator protein fused to a known protein, in this case an inventive protein, and the other contains the activator protein's activation domain fused to an unknown protein (a putative GAP, for instance) that is encoded by a cDNA which has been recombined into this plasmid as part of a cDNA library. The plasmids are transformed into a strain of the yeast Saccharomyces cerevisiae that contains a reporter gene (e.g., lacZ) whose regulatory region contains the transcription activator's binding sites. Either hybrid protein alone cannot activate transcription of the reporter gene, the DNA-binding domain hybrid cannot because it does not provide activation function, and the activation domain hybrid cannot because it cannot localize to the activator's binding sites. Interaction of the two hybrid proteins reconstitutes the functional activator protein and results in expression of the reporter gene, which is detected by an assay for the reporter gene product. The two-hybrid system or related methodology can be used to screen activation domain libraries for proteins that interact with a known "bait" gene product. By way of example, and not by way of limitation, gene products known to be involved in TH cell subpopulation-related disorders and/or differentiation, maintenance, and/or effector function of the subpopulations can be used as the bait gene products. Total genomic or cDNA sequences are fused to the DNA encoding on activation domain. This library and a plasmid encoding a hybrid of the bait gene product fused to the DNA-binding domain are cotransformed into a yeast reporter strain, and the resulting transformants are screened for those that express the reporter gene. For example, and not by way of limitation, the bait gene can be cloned into a vector such that it is translationally fused to the DNA encoding the DNA- binding domain of the GAL4 protein. These colonies are purified and the library plasmids responsible for reporter gene expression are isolated. DNA sequencing is then used to identify the proteins encoded by the library plasmids. The present invention, thus generally described, will be understood more readily by reference to the following examples, which are provided by way of illustration and are not intended to be limiting of the present invention. The examples below are provided to illustrate the subject invention. These examples are provided by way of illustration and are not included for the purpose of limiting the invention. EXAMPLES EXAMPLE I: cDNA Library Construction cDNA library plates and clones originated from five cDNA libraries that were constructed by directional cloning. These are available through the Resource Center (http://www.rzpd.de) of the German Genome Project. In particular, the hfbr2 (human fetal brain; RZPD number DKFZp564) and hfkd2 (human fetal kidney; DKFZp566) libraries were generated using the Smart kit (Clontech), except that PCR was carried out with primers that contained uracil residues to permit directional cloning without restriction digestion and ligation, and were complementary with the pAMPl (LifeTechnologies) cloning sites for directional cloning. The htes3 (human testes; DKFZp434), hutel (human uterus; DKFZp586) and hmcfl (human mammary carcinoma; DKFZp727) libraries are conventional (Gubler, U., Hoffman, B.J., (1983), A simple and very efficient method for generating cDNA libraries. Gene 25, 263-269), size-selected cDNA libraries. They are cloned into pSPORTl (LifeTechnologies) via a Notl site which is introduced during reverse transcription downstream of the oligo dT primer and a Sail site that is introduced by the ligation of a adapters. The human mammary carcinoma library was constructed fgrom MCF7 cells. The cDNA sequences of this application were first identified among the sequences comprising various libraries. Technology has advanced considerably since the first cDNA libraries were made. Many small variations in both chemicals and machinery have been instituted over time, and these have improved both the efficiency and safety of the process. Although the cDNAs could be obtained using an older procedure, the procedure presented in this application is exemplary of one currently being used by persons skilled in the art. For the purpose of providing an exemplary method, the mRNA isolation and cDNA library construction described here is for the MCF-7 library (DKFZp727) from which the clones named DKFZphmcfl xxyyxx were obtained. The human cell line MCF-7 was grown in DMEM supplemented with 10% fetal calf serum until confluency. 3 X 10 8 cells were harvested with a cell scraper in PBS. Cells were lysed in buffer containing 0.5 % NP-40 to leave the nuclei intact. The debris was pelleted by centrifugation at 15 000 x g for 10 minutes at 4 degrees Celsius. Proteins in the supernatant were degraded in presence of SDS and Proteinase K (30 minutes at 56 degrees Celsius). Precipitation of proteins was done in a Phenol/Chloroform extraction, RNA was precipitated from the aqueous phase with Na-acetate and Ethanol. Polyadenylated messages were isolated using Qiagen Oligotex (QIAGEN, Hilden Germany). First strand cDNA synthesis was accomplished using an oligo (dT) primer which also contained an Notl restriction site. Second strand synthesis was performed using a combination of DNA polymerase I, E. coli ligase and RNase H, followed by the addition of a Sail adaptor to the blunt ended cDNA. The Sail adapted, double-stranded cDNA was then digested with Notl restriction enzyme, and fractionated by size on an agarose gel. DNA of the appropriate size was cut from the gel and cast into a second gel in a 90° angle. After electrophoresis in the second dimension, cDNA of the appropriate size was cut from the gel. The agarose block was broken down with help of gelase. The cDNA was purified with help of two phenol extractions and an ethanol precipitation. The cDNA was ligated into Sall/Notl pre-digested pSportl vector (LifeTechnologies) and transformed into DH10B bacteria. The libraries were arrayed into 384-well microtiter plates and spotted on high density nylon membranes for hybridization analysis. Filters and clones are available through the Resource Center. Whole plates were distributed to the sequencing partners of the consortium for systematic sequencing. EXAMPLE II: Sequencing of cDNA Clones All clones in the 384-well microtiter plates were sequenced from the 5' end. Sequencing was done preferentially using dye terminator chemistry (ABD or Amersham) on ABI automated DNA sequencers (ABI 377, Applied Biosystems), one partner used EMBL prototype instruments (Arakis) mainly with dye primer chemistry. The resulting expressed sequence tag (EST) sequences ("rl ESTs" = sequenced from 5 '-end) were analysed for: a) the lack of identical matches with known genes. For this, the EST-sequence was blasted against the cDNA consortiums own database and after that against public databases and (with BLASTn and BLASTx against EMBL/EMBLNEW and assembled ESTs, please refer to EXAMPLE III: Bioinformatics analysis of full length cDNAs, for description and parameter settings). ESTs which were identical to known genes in more than 100 bp, with less than 2 mismatches, were excluded from further analysis. b) the presence of an open reading frame Open reading frames (ORFs) were detected with an tool developed by Munich Information Center for Protein Sequences (MIPS) called ORF-map. ORF-map visualises potential start and stop-codons. If an ORF without a stop codon was detected in a rl-EST, the sequence was processed further. c) the presence of GC rich sequences A script developed by MIPS computed the GC -content of the rl -sequence, which should be >40%. Writing similar scripts is within the ordinary skill of one in bioinformatics. d) the lack of repeat structures Repeats such as Alu, Line or CA-repeats were detected by blasting (BLASTn and BLASTx, please refer to EXAMPLE III: Bioinformatics analysis of full length cDNAs, for description and parameter settings) against a repeat-database compiled by MIPS. If a repeat was present within the rl -sequence, the sequence were not processed further. Novel clones that met all criteria were identified to the sequencers, who then performed 3 '-end sequencing of these clones. The resulting 3' ESTs ("si ESTs" = sequenced from 3 '-end) were checked for a) the lack of matches with known genes in public databases, and sequences already generated by us. This was done by blasting against EMBL/EMBLNEW and assembled EST (BLASTn and BLASTx, please refer to EXAMPLE III: Bioinformatics analysis of full length cDNAs, for description and parameter settings). b) the presence of polyadenylation signals. Again only clones matching the selection criteria were chosen to be sequenced completely by the sequencers. Clones were selected after the following criteria: A very good ORF had at least one BLASTx match to other proteins. A "good ORF" should extend to the 3' end and be longer than ~40 codons. If the ORF started in the rl sequence, in front of the potential start codon, there should not exist too many competing start codons in frame with the ORF start codon and the start should match the Kozak consensus ATG. If the EST sequence was to short to decide according to the potential ORF, and there were only a few or no start codons in the sequence the GC content of the Sequence should be greater than 40%. The rl sequences needed not contain an polyA-tail at the 3' end. In addition, the results of the blasting against the assembled human ESTs could help in questionable cases to decide whether to stop or to continue. A hit against these ESTs was an indication to go further. Clones passing the above-described screening were sequenced in full. Sequencing was done preferentially using dye terminator chemistry (ABD or Amersham) on ABI automated DNA sequencers (ABI 377, Applied Biosystems), one partner used EMBL prototype instruments (Arakis) mainly with dye primer chemistry. Primer walking (Strauss et al., 1986, Specific-primer-directed DNA sequencing. Anal Biochem. 154, 353-360) was the preferred sequencing strategy because of the lower redundancy possible compared to random shotgun (Messing, J., Crea, R., Seeburg, H.P. (1981) A system for shotgun DNA sequencing. Nucleic Acids Res. 9, 32-39) methods. Walking primers were generally designed using software (e.g. Haas, S., Vingron, M., Poustka, A., Wiemann, S. (1998) Primer design in large-scale sequencing. Nucleic Acids Res. 26, 3006-3012, Schwager, C, Wiemann, S., Ansorge, W. (1995) GeneSkipper: integrated software environment for DNA sequence assembly and alignment. HUGO Genome Digest 2, 8-9) that permitted complete automation of this usually time consuming process and helped in the parallel processing of large numbers of clones. EXAMPLE III: Bioinformatics analysis of full length cDNAs Each sequence obtained was compared on nucleotide level in a stepwise manner to sequences in EMBL/EMBLNEW, EMBL-EST, EMBL-STS using the BLASTn algorithm. Basic Local Alignment Search Tool (BLAST, Altschul S. F. (1993) J Mol Evol 36:290-300; Altschul, S. F. et al (1990) J Mol Biol 215:403-10) is used to search for local sequence alignments. BLAST produces alignments of both nucleotide (BLASTn) and amino acid sequences (BLASTp or BLASTx) to determine sequence similarity. BLAST is especially useful in determining exact matches or in identifying homologs, because of the local nature of the alignments. While it is useful for matches which do not contain gaps, it is inappropriate for performing motif-style searching. The fundamental unit of BLAST algorithm output is the High-scoring Segment Pair (HSP). An HSP consists of two sequence fragments of arbitrary but equal lengths whose alignment is locally maximal and for which the alignment BLAST approach is to look threshold or cut off score set by the user. BLAST looks for HSPs between a query sequence and a database sequence, to evaluate the statistical significance of any matches found, and to report only those matches which satisfy the user-selected threshold of significance. The parameter E establishes the statistically significant threshold for reporting database sequence matches. E is interpreted as the upper bound of the expected frequency of chance occurrence of an HSP (or set of HSPs) within the context of the entire database search. Any database sequence whose match satisfies E is reported in the program output. Parameter settings for the BLAST-operations (BLASTN 2.0al9MP-WashU) described were: EMBL-EMBLNEW: H=0 V=5 B=5 -filter seg; EMBL-EST: H=0 E=le-10 B=500 V=500 -filter seg; EMBL-STS: H=0 V=5 B=5. Search against EMBL/EMBLNEW was done to determine whether the cDNAs are already known, and also to find out whether the cDNAs are encoded by genomic sequences already sequenced and published/submitted to these databases. Search against EMBL-EST was performed to get a first impression how abundant a particular cDNA would be and to get information on tissue specificity (so-called "electronic Northern-Blot", e.g. some of the cDNAs derived of the testis library show only hits to ESTs also derived of testis libraries). The cDNA-sequences were blasted against EMBL-STS to determine STS-sequence- match to the cDNA, thus providing a mapping information to the new cDNA. The potential protein-sequences were generated automatically by a script searching for the longest open reading frame (ORF) in each of the three forward frames with a minimum length of 90 codons. Next, the automatically generated ORFs were translated into protein sequences. These protein sequences were searched against the non redundant protein data set of PIR/SwissProt/Trembel/Tremblnew (BLASTP 2.0al9MP-WashU, parameter setting: V=7 B-7 H=0 -filter seg). If the script generated more than one ORF, one ORF was chosen manually by the annotater according to the degree of similarity to known proteins, the location of the ORF in the cDNA, the length, the amino acid composition and the content of Prosite-Motifs. Additionally there was a BLASTx (BLASTX 2.0al9MP-WashU against non redundant protein database comprising PIR/SWISSPROT/TREMBL/TREMBLNEW; parameter-settings were: matrix/home/data/blast/matrix aa/BLOSUM62 H=0 V=5 B=5 -filter seg) search to find potential frame shift in the complementary eds of the cDNAs and to identify unspliced or partly spliced cDNAs. The protein sequence was then transferred to the PEDANT system, in order to generate additional information on the new proteins. PEDANT (Protein Extraction, Description, and ANalysis Tool, Frishman, D. & Mewes, H.-W. (1997) PEDANTic genome analysis. Trends in Genetics , 13, 415-416) is a platform developed at the Munich Information Center for Protein Sequences (MIPS, Munich, Germany), which incorporates practically all bioinformatics methods important for the functional and structural characterisation of protein sequences. Computational methods used by PEDANT are: FASTA Very sensitive protein sequence database searches with estimates of statistical significance. Pearson W.R. (1990) Rapid and sensitive sequence comparison with FASTP and FASTA. Methods Enzymol. 183, 63-98. BLAST2 Very sensitive protein sequence database searches with estimates of statistical significance. Altschul S.F., Gish W., Miller W., Myers E.W., and Lipman D.J. Basic local alignment search tool. Journal of Molecular Biology 215, 403-10. PREDATOR High-accuracy secondary structure prediction from single and multiple sequences. Frishman, D. and Argos, P. (1997) 75% accuracy in protein secondary structure prediction. Proteins, 27, 329-335. Frishman, D. and Argos, P.(1996) Incorporation of long-distance interactions in a secondary structure prediction algorithm. Prot. Eng. 9, 133-142. STRIDE Secondary structure assignment from atomic coordinates. Frishman, D. and Argos, P. (1995) Knowledge-based secondary structure assignment. Proteins 23, 566-579. CLUSTALW Multiple sequence alignment. Thompson, J.D., Higgins, D.G. and Gibson, T.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22:4673-4680. TMAP Transmembrane region prediction from multiply aligned sequences. Persson, B. and Argos, P. (1994) Prediction of transmembrane segments in proteins utilising multiple sequence alignments. J. Mol. Biol. 237, 182-192. ALOM2 Transmembrane region prediction from single sequences. Klein, P., Kanehisa, M., and DeLisi, C. Prediction of protein function from sequence properties: A discriminant analysis of a database. Biochim. Biophys. Acta 787, 221-226 (1984). Version 2 by Dr. K. Nakai. SIGNALP Signal peptide prediction Nielsen, H., Engelbrecht, J., Brunak, S., and von Heijne, G (1997). Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Engineering 10, 1-6. SEG Detection of low complexity regions in protein sequences. Wootton, J.C., Federhen, S. (1993) Statistics of local complexity in amino acid sequences and sequence databases. Computers & Chemistry 17, 149-163. COILS Detection of coiled coils. Lupas, A., M. Van Dyke, and J. Stock, "Predicting Coiled Coils from Protein Sequences." Science (1991) 252, 1162-1164. PROSEARCH Detection of PROSITE protein sequence patterns. Kolakowski L.F. Jr., Leunissen J.A.M., Smith J.E. (1992) ProSearch: fast searching of protein sequences with regular expression patterns related to protein structure and function. Biotechniques 13, 919-921. BLIMPS Similarity searches against a database of ungapped blocks. J.C. Wallace and Henikoff S., (1992) PATMAT: a searching and extraction program for sequence, pattern and block queries and databases, CABIOS 8, 249-254. Written by Bill Alford. HMMER Hidden Markov model software . Sonnhammer E.L.L., Eddy S.R., Durbin R. (1997) Pfam: A Comprehensive Database of Protein Families Based on Seed Alignments. Proteins 28, 405-420. pi Perl script that returns the amino acid composition, molecular weight, theoretical pi, and expected extinction coefficient of an amino acid sequence. By Fred Lindberg. The parameter-settings were as follows: known3d: score > 100; BLAST: E-value < 10; SCOP: <= 50 Alignments, E-Value < 0.0001 ; signalp: Y=0.7; untersucht vom N-Terminus her: 50 aa; funcat: E-value < 0.001; BLOCKS: <= 10 hits; BLIMPS: threshold 1100.0; COILS: threshold 0.95; SEG: threshold 20.0; BLAST in report: E-value < 0.001; PIR-KW, superfamilies, EC- Nummern in report: E-value < 0.00001; known3d in report: score > 120 The results of PEDANT analysis, together with the results of the similarity searches, constitute the basis for the structural and functional annotation of the cDNAs and the encoded proteins, as specified below. EXAMPLE III: CELLULAR LOCALIZATIONS OF GFP-FUSION PROTEINS Plasmids of cDNA-GFP fusions were transfected into mammalian tissue culture cells and allowed to express the proteins for up to 48 hours. Live cells were imaged at 24 hours and 48 hours after transfection and the localisations recorded. The chart, below, depicts the apparent final cellular localisations of 107 cDNA-GFP fusions. In order to minimize the possibility of the GFP interfering with protein function and/or localization, two separate populations of cDNAs were generated encoding N-terminal or C-terminal GFP fusions. Clearly this appears to be a crucial strategy, since overall only 56% of the proteins localised to a specific compartment irrespective of the position of the GFP. In the instances where only one fusion localized, the complementary fusion either gave no expression or a nuclear and cytosolic staining - characteristic for GFP alone expression. Each cDNA in turn was subjected to bioinformatic analysis. Where possible, the potential subcellular localisations of the expressed proteins were determined. This information was then compared to the actual localisations determined from expression of the GFP-fusion proteins in mammalian cells. DKFZphfbr 2 _16cl6 group: Cell structure and motility DKFZphfbr 2 _16cl6 , 3 encodes a novel 586 amino acid 1 protein with .similarity to the human actin binding protein MAYVEN and Drosophila Kelch. AVEN is a novel actin binding protein predominantly expressed in brain. Drosophila kelch is involved in the maintenance of ring canal organization during oogenesis. The amino half of the protein including the BTB domain mediates dimerization, while the amino half might allow cross-linking of ring canal actin filaments, thus organising the inner rim cytoskeleton. The kelch repeat domain is necessary for ring canal localisation and believed to mediate an additional interaction, possibly with actin. The new protein shares the features of both proteins and therefore should be involved in the organisation of cyto skeleton binding to membrane proteins . The new protein can find application in modulating/blocking of cyto skeleton-membrane protein interaction. similarity to Drosophila kelch complete cDNA, complete eds, EST hits on genomic level partly encoded by AC00508 and AC006039 Sequenced by Qiagen Locus : unknown Insert length: 3 028 bp Poly A stretch at pos . 3 004, polyadenylation signal at pos . 2984 1 GGGGGCCCGG GGACGCAGCC CAGTTGGTAG CGTCGCTCCC TGAGCGTTTC 51 TAAGGGGGCC GCCCGGCCCT GTCTTTCGGC AGTGGCCGAG CCACCGCCGC 101 CTGCCGCGCG TTCCAGAGCT GGGCGCTGCA GCTGCACTGC CGATCGCCGT 151 GTTTGGTCGA TAGAATCCCC AGTGTGCCCA GAGAGTGCGA CCCCTCGCCC 201 GGCCCGGCGA GCCCCGGGCG TGAACCGAGC TGAGGGAGGA TGGCAGCCTC 251 TGGGGTGGAG AAGAGCAGCA AGAAGAAGAC CGAGAAGAAA CTTGCTGCTC 301 GGGAAGAAGC TAAATTGTTG GCGGGTTTCA TGGGCGTCAT GAATAACATG 351 CGGAAACAGA AAACGTTGTG TGACGTGATC CTCATGGTCC AGGAAAGAAA 401 GATACCTGCT CATCGTGTTG TTCTTGCTGC AGCCAGTCAT TTTTTTAACT 451 TAATGTTCAC AACTAACATG CTTGAATCAA AGTCCTTTGA AGTAGAACTC 501 AAAGATGCTG AACCTGATAT TATTGAACAA CTGGTGGAAT TTGCTTATAC 551 TGCTAGAATT TCCGTGAATA GCAACAATGT TCAGTCTTTG TTGGATGCAG 601 CAAACCAATA TCAGATTGAA CCTGTGAAGA AAATGTGTGT TGATTTTTTG 651 AAAGAACAAG TTGATGCTTC AAATTGTCTT GGTATAAGTG TGCTAGCGGA 701 GTGTCTAGAT TGTCCTGAAT TGAAAGCAAC TGCAGATGAC TTTATTCATC 751 AGCACTTTAC TGAAGTTTAC AAAACTGATG AATTTCTTCA ACTTGATGTC 801 AAGCGAGTAA CACATCTTCT CAACCAGGAC ACTCTGACTG TGAGAGCAGA 851 GGATCAGGTT TATGATGCTG CAGTCAGGTG GTTGAAATAC ' GATGAGCCTA 901 ATCGCCAGCC ATTTATGGTT GATATCCTTG CTAAAGTCAG GTTTCCTCTT 951 ATATCAAAGA ATTTCTTAAG TAAAACGGTA CAAGCTGAAC CACTTATTCA 1001 AGACAATCCT GAATGCCTTA AGATGGTGAT AAGTGGAATG AGGTACCATC 1051 TACTGTCTCC AGAGGACCGA GAAGAACTTG TAGATGGCAC AAGACCTAGA 1101 AGAAAGAAAC ATGACTACCG CATAGCCCTA TTTGGAGGCT CTCAACCACA 1151 GTCTTGTAGA TATTTTAACC CAAAGGATTA TAGCTGGACA GACATCCGCT 1201 GCCCCTTTGA AAAACGAAGA GATGCAGCAT GCGTGTTTTG GGACAATGTA 1251 GTATACATTT TGGGAGGCTC TCAGCTTTTC CCAATAAAGC GAATGGACTG 1301 CTATAATGTA GTGAAGGATA GCTGGTATTC GAAACTGGGT CCTCCGACAC 1351 CTCGAGACAG CCTTGCTGCA TGTGCTGCAG AAGGCAAAAT TTATACATCT 1401 GGAGGTTCAG AAGTAGGAAA CTCAGCTCTG TATTTATTTG AGTGCTATGA 1451 TACGAGAACT GAAAGCTGGC ACACAAAGCC CAGCATGCTG ACCCAGCGCT 1501 GCAGCCATGG GATGGTGGAA GCCAATGGCC TAATCTATGT TTGTGGTGGA 1551 AGTTTAGGAA ACAATGTTTC AGGGAGAGTG CTTAATTCCT GTGAAGTTTA 1601 TGATCCTGCC ACAGAAACAT GGACTGAGCT GTGTCCAATG ATTGAAGCCA 1651 GGAAGAATCA TGGGCTGGTA TTTGTAAAAG ACAAGATATT TGCTGTGGGT 1701 GGTCAGAATG GTTTAGGTGG TCTGGACAAT GTGGAATATT ACGATATTAA 1751 GTTGAACGAA TGGAAGATGG TCTCACCAAT GCCATGGAAG GGTGTAACAG 1801 TGAAATGTGC AGCAGTTGGC TCTATAGTTT ATGTCTTGGC TGGTTTTCAG 2 3 01 AGAAGATTGG CTCATCAGTG AAGCGCAGTA TCTTAGCTCT AGATTCTATT 23 51 TTCATGCATC ACAGAAGTGC TATACGGTTA GGTCTGTTTG TGCTCAGTCA 2 401 AGAACTAAGA AATAGTATGA ATTGTAAGTC AAGATGGGCA ACTCAGATGG 2 451 AGCAGCTTAG TCTCACAGTT TGCTTGTCTA TTTATTTTAT TTAGTGCCAA 2501 ATGTATTCCA TTTTAAAAGT AAGCCAGAGT GAGTCAAGGC ATATACACAC 2 551 TTTCTCACAA AACTTCCTAA ACAGATTTGG GGGTTTAATA TGTCCAACTC 2 601 CTCATGAAAT ATATTCAATC CACTTAAATA TATTCCATCT TTTTAACATA 2 651 AAATGTAAAG CTTAGCACCC ATCATTAATT TATGTCTCTG TTTTATCCAG 2701 TGGTTAAAAA AGGATTCTGC CTCTTTAGTC CTCACTGTTA AATAAAACCC 2751 AATCATAGTA AGTGATTAAC TAGCAAAAAG TAAAGCTATT TATAGCAAAT 2801 TTCTAGATCA TTAGAAAAGC ACTGGTAGTT GTACAATATC AGTGTTGACT 2851 TTGAACTTCT TTAACGAGAT CATGAATTCT TTTCCCTTAG CCAAAACATG 2901 AAATATTTAA CCTAGTTGTC TCTAAAAGTT TTGTAATCAT GAGTTAGATA 2951 TATGTCATCT CCTATTCATT GCTTTTATGT GATCAATAAA TCTTTTACAA 3001 ACCCAAAAGA AAAAAAAAAA AAAAAAAA BLAST Results Entry AC005082 from database EMBL: Homo sapiens clone RG271G13; HTGS phase 1, 7 unordered pieces. Score = 6460, P = O.Oe+00, identities = 1292/1292 4 exons matching Bp 1180-3007 Entry AC006039 from database EMBL: *** SEQUENCING IN PROGRESS *** Homo sapiens clone NH0319F03; HTGS phase 1, 3 unordered pieces. Score = 1780, P = 2.0e-117, identities = 368/377 5 exons matching Bp 6-860 Entry HSG20603 from database EMBL: human STS A005Y34. Score = 670, P = 1.0e-23, identities = 134/134 Medline entries 93201592: kelch encodes a component of intercellular bridges in Drosophila egg chambers . 97412177: Drosophila kelch is an oligomeric ring canal actin organizer. Peptide information for frame 3 ORF from 240 bp to 1997 bp; peptide length: 586 Category: strong similarity to known protein 1 MAASGVEKSS KKKTEKKLAA REEAKLLAGF MGVMNNMRKQ KTLCDVILMV 51 QERKIPAHRV VLAAASHFFN LMFTTNMLES KSFEVELKDA EPDIIEQLVE 101 FAYTARISVN SNNVQSLLDA ANQYQIEPVK KMCVDFLKEQ VDASNCLGIS 151 VLAECLDCPE LKATADDFIH QHFTEVYKTD EFLQLDVKRV THLLNQDTLT 201 VRAEDQVYDA AVRWLKYDEP NRQPFMVDIL AKVRFPLISK NFLSKTVQAE 251 PLIQDNPECL KMVISGMRYH LLSPEDREEL VDGTRPRRKK HDYRIALFGG 301 SQPQSCRYFN PKDYS TDIR CPFEKRRDAA CVFWDNVVYI LGGSQLFPIK 351 RMDCYNVVKD S YSKLGPPT PRDSLAACAA EGKIYTSGGS EVGNSALYLF 401 ECYDTRTES HTKPSMLTQR CSHGMVEANG LIYVCGGSLG NNVSGRVLNS 451 CEVYDPATET WTELCPMIEA RKNHGLVFVK DKIFAVGGQN GLGGLDNVEY 501 YDIKLNE KM VSPMPWKGVT VKCAAVGSIV YVLAGFQGVG RLGHILEYNT 551 ETDKWVANSK VRAFPVTSCL ICVVDTCGAN EETLET BLASTP hits Entry KELC_DROME from database SWISSPROT: RING CANAL PROTEIN (KELCH PROTEIN) . Length = 689 Score = 816 (287.2 bits), Expect = 1.9e-81, P = 1.9e-81 Identities = 187/542 (34%), Positives = 290/542 (53%) Entry AC004021_1 from database TREMBL: WUGSC:H_DJ0186K10.1"; Human PAC clone DJ0186K10 from 5q31, complete sequence. Homo sapiens (human) Length = 497 Score = 704 (247.8 b ts). Expect = 1.4e-69, P = 1.4e-69 Identities = 163/483 (33%), Positives = 253/483 (52%) Entry HSDKG12_1 from database TREMBL: "KIAA0132"; Human mRNA for KIAA0132 gene, complete eds. Homo sapiens (human) Length = 624 Score = 692 (243.6 bits), Expect = 2.6e-68, P = 2.6e-68 Identities = 175/527 (33%), Positives = 272/527 (51%) Entry A45773 from database PIR: kelch protein, long form - fruit fly (Drosophila melanogaster) Length = 1476 Score = 817 (287.6 bits), Expect = 1.7e-80, P = 1.7e-80 Identities = 189/549 (34%), Positives = 292/549 (53%) Alert BLASTP hits for DKFZphfbr2_16cl6, frame 3 No Alert BLASTP hits found Pedant information for DKFZphfbr2_16cl6, frame 3 Report for DKFZphfbr2_16cl6.3 [LENGTH] 586 [MW] 65992.06 [pi] 6.08 [HOMOL] PIR:A45773 kelch protein, long form - fruit fly (Drosophila melanogaster) 5e-85 [BLOCKS] BL00075D Dihydrofolate reductase proteins [SCOP] dlgog_3 2.46.1.1.1 (151-537) Galactose oxidase, central domai 6e-36 [PIRK ] zinc finger 2e-ll [PIRK ] DNA binding 9e-10 [PIRKW] transcription factor le-06 [SUPFAM] A55R protein middle region homology le-35 [SUPFAM] POZ domain homology le-35 [SUPFAM] vaccinia virus 59K Hindlll-C protein 5e-15 [SUPFAM] A55R protein le-35 [SUPFAM] myxoma virus M9-R protein 2e-ll [SUPFAM] A55R protein carboxyl-terminal homology le-35 [PROSITE] CAMP_PHOSPHO_SITE 2 [PROSITE] MYRISTYL 8 [PROSITE] CK2_PHOSPHO_SITE 10 [PROSITE] TYR_PHOSPHO_SITE 1 [PROSITE] PKC_PHOSPHO_SITE 11 [PROSITE] ASN_GLYCOSYLATION 1 [KW] Alpha_Beta [KW] LOW COMPLEXITY 3.75 % SEQ MAASGVEKSSKKKTEKKLAAREEAKLLAGFMGVMNNMRKQKTLCDVILMVQERKIPAHRV SEG xxxxxxxxxxxxxxxxxxxxxx PRD ccceeeeccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhheeeeccccchhhhhe SEQ VLAAASHFFNLMFTTNMLESKSFEVELKDAEPDIIEQLVEFAYTARISVNSNNVQSLLDA SEG PRD eeccccccccccccccchhhhhheeeeccccchhhhhhhhhhhhheeeeccchhhhhhhh SEQ ANQYQIEPVKKMCVDFLKEQVDASNCLGISVLAECLDCPELKATADDFIHQHFTEVYKTD SEG PRD hhhhhhhhhhhhhhhhhhhhhcccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh SEQ EFLQLDVKRVTHLLNQDTLTVRAEDQVYDAAVRWLKYDEPNRQPFMVDILAKVRFPLISK SEG PRD hhhchhhhhhhhhhhcccccchhhhhhhhhhhhhhhhhcccchhhhhhhhhhhhhhccch SEQ NFLSKTVQAEPLIQDNPECLKMVISGMRYHLLSPEDREELVDGTRPRRKKHDYRIALFGG SEG PRD hhhhhhhhhhhccccccchhhhhhhhhhhhccccccccccccccccccccceeeeeeecc SEQ SQPQSCRYFNPKDYSWTDIRCPFEKRRDAACVFWDNVVYILGGSQLFPIKRMDCYNVVKD SEG PRD ccccceeeccccccccccccccccccceeeeeeeceeeeeeccccccccceeeecccccc SEQ SWYSKLGPPTPRDSLAACAAEGKIYTSGGSEVGNSALYLFECYDTRTESWHTKPSMLTQR SEG PRD cccccccccccccceeeeeccceeeeeccccccccceeeeeecccccccccccccccccc SEQ CSHGMVEANGLIYVCGGSLGNNVSGRVLNSCEVYDPATETWTELCPMIEARKNHGLVFVK SEG PRD ccceeeecceeeeeecccccccccccccceeeeccccccccccccccccccccceeeeec SEQ DKIFAVGGQNGLGGLDNVEYYDIKLNEWKMVSPMPWKGVTVKCAAVGSIVYVLAGFQGVG SEG PRD ceeeecccccccccccceeeccccccceeecccccccccceeeeeccceeeeeccccccc SEQ RLGHILEYNTETDKWVANSKVRAFPVTSCLICVVDTCGANEETLET SEG PRD cccceeecccccccccccccccccccceeeeeeeeccccccccccc Prosite for DKFZphfbr2_16cl6.3 PS00001 442->446 ASN_GLYCOΞYLATION PDOC00001 PS00004 11->15 CAMP_PHOSPHO_SITE PDOC00004 PS00004 188->192 CAMP_PHOSPHO_SITE PDOC00004 PS00005 9->12 PKC_PHOSPHO_SITE PDOC00005 PS00005 10->13 PKC_PHOSPHO_SITE PDOC00005 PS00005 14->17 PKC_PHOSPHO_SITE PDOC00005 PS00005 104->107 PKC_PHOSPHO_SITE PDOC00005 PS00005 200->203 PKC_PHOSPHO_SITE PDOC00005 PS00005 305->308 PKC_PHOSPHO_SITE PDOC00005 PS00005 370->373 PKC_PHOSPHO_SITE PDOC00005 PS00005 418->421 PKC_PHOSPHO_SITE PDOC00005 PS00005 444->447 PKC_PHOSPHO_ΞITE PDOC00005 PS00005 520->523 PKC_PHOSPHO_SITE PDOC00005 PS00005 ,552->555 PKC_PHOSPHO_SITE PDOC00005 PS00006 4->8 CK2_PHOSPHO_SITE PDOC00006 PS00006 42->46 CK2_PHOSPHO_SITE PDOC00006 PS00006 116->120 CK2_PHOSPHO_SITE PDOC00006 PS00006 164->168 CK2_PHOSPHO_SITE PDOC00006 PS00006 273->277 CK2_PHOSPHO_SITE PDOC00006 PS00006 315->319 CK2_PHOSPHO_SITE PDOC00006 PS00006 370->374 CK2_PHOSPHO_SITE PDOC00006 PS00006 405->409 CK2_PHOSPHO_SITE PDOC00006 PS00006 460->464 CK2_PHOSPHO_SITE PDOC00006 PS00006 550->554 CK2_PHOSPHO_SITE PDOC00006 PS00007 202->209 TYR_PHOSPHO_SITE PDOC00007 PΞ00008 5->ll MYRISTYL PDOC00008 PΞ00008 32->38 MYRISTYL PDOC00008 PS00008 389->395 MYRISTYL PDOC00008 PS00008 424->430 MYRISTYL PDOC00008 PS00008 436->442 MYRISTYL PDOC00008 PS00008 440->446 MYRISTYL PDOC00008 PS00008 487->493 MYRISTYL PDOC00008 PS00008 493->499 MYRISTYL PDOC00008 (No Pfam data available for DKFZphfbr2_16cl6.3) DKFZphfbr2_16f21 group: brain derived DKFZphfbr2_16f21 encodes a novel 208 ammo acid protein with strong similarity to human zinc finger protein 216. The novel protein shows strong similarity to the human zinc finger protein 216, but has no Zn finger. PROSITE: Contains no Zinc finger; No informative BLAST results; no predictive prosite, pfam or SCOP motife The new protein can find application in studying the expression profile of bram-specific genes . strong similarity to zinc finger protein 216 complete cDNA, complete eds, EST hits start matches Kozak consensus ANNatgG, Sequenced by Qiagen Locus : unknown Insert length: 1512 bp Poly A stretch at pos. 1490, polyadenylation signal at pos. 1474 1 GGGAGCAAGC AGGGGTTCGG CGGCATTACC TGTACCCATT CACCGGCGGC 51 TACCGGCGGC GGCGCGTAGC GTGTCAGGCG GAGAGACCCG CCGCCAGGTG 101 TGCAACTGAG GAACATGGCT CAAGAAACTA ATCACAGCCA AGTGCCTATG 151 CTTTGTTCCA CTGGCTGTGG ATTTTATGGA AACCCTCGTA CAAATGGCAT 201 GTGTTCAGTA TGCTATAAAG AACATCTTCA AAGACAGAAT AGTAGTAATG 251 GTAGAATAAG CCCACCTGCA ACCTCTGTCA GTAGTCTGTC TGAATCTTTA 301 CCAGTTCAAT GCACAGATGG CAGTGTGCCA GAAGCCCAGT CAGCATTAGA 351 CTCTACATCT TCATCTATGC AGCCCAGCCC TGTATCAAAT CAGTCACTTT 401 TATCAGAATC TGTAGCATCT TCTCAATTGG ACAGTACATC TGTGGACAAA 451 GCAGTACCTG AAACAGAAGA TGTGCAGGCT TCAGTATCAG ACACAGCACA 501 GCAGCCATCT GAAGAGCAAA GCAAGCCTCT TGAAAAACCG AAACAAAAAA 551 AGAATCGCTG TTTCATGTGC AGGAAGAAAG TGGGACTTAC TGGGTTTGAA 601 TGCCGGTGTG GAAATGTTTA CTGTGGTGTA CACCGTTACT CAGATGTACT 651 CAATTGCTCT TACAATTACA AAGCCGATGC TGCTGAGAAA ATCAGAAAAG 701 AAAATCCAGT AGTTGTTGGT GAAAAGATCC AAAAGATTTG AACTCCTGCT 751 GGAATACAAA ATTCTTGAGC ATCTGCAAAC TAAAAATTGA CTTGAGGTTT 801 TTTTTTTCCT AGTCATTGGG AATGTAGAGC AGTGTATCTT GCATGTCATC 851 GGAAGAATAG ATTTTTGTTT TGGTTTTGTT TTGAAAATGA CTCTGAACAT 901 TTATTTCCAT TGCAATTTCT GTGGCTGAGG AGACTTAAAC TTTACAAGTA 951 TTATCCTTTT AAGATCATTT TAATTTTAGT TGAGTGCAGA GGGCTTTTAT 1001 AACAAACGTG CAGAAATTTT GGAGGGCTGT GATTTTTCCA GTATTAAACA 1051 TGCATGCATT AATCTTGCAG TTTATTTTCT CATTATGTAT GTATATATCG 1101 CTTTTCTCTG CAGCACGATT TCTCTTTTGA TAATGCCCTT TAGGGCACAA 1151 CTAGTTATCA GTAACTGAAT GTATCTTAAT CATTATGGCT GCTTCTGTTT 1201 TTTCATTAAC AAAGGTTATT CATATGTTAG CATATAGTTT CTTTGCACCC 1251 ACTATTTATG TCTGAATCAT TTGTCACAAG AGAGTGTGTG CTGATGAGAT 1301 TGTAAGTTTG TGTGTTTAAA CTTTTTTTTG AGCGAGGGAA GAAAAAGCTG 1351 TATGCATTTC ATTGCTGTCT ACAGGTTTCT TTCAGATTAT GTTCATGGGT 1401 TTGTGTGTAT ACAATATGAA GAATGATCTG AAGTAATTGT GCTGTATTTA 1451 TGTTTATTCA CCAGTCTTTG ATTAAATAAA AAGGAAAACC AGAAAAAAAA 1501 AAAAAAAAAA AA BLAST Results No BLAST result Medlme entries o Medlme entry Peptide information for frame 1 ORF from 115 bp to 738 bp; peptide length: 208 Category: strong similarity to known protein 1 MAQETNHSQV PMLCSTGCGF YGNPRTNGMC SVCYKEHLQR QNSSNGRISP 51 PATSVSSLSE SLPVQCTDGS VPEAQSALDS TSSSMQPSPV SNQSLLSESV 101 ASSQLDSTSV DKAVPETEDV QASVSDTAQQ PSEEQSKPLE KPKQKKNRCF 151 MCRKKVGLTG FECRCGNVYC GVHRYSDVLN CSYNYKADAA EKIRKENPVV 201 VGEKIQKI BLASTP hits Entry ATF7H19_1 from database TREMBLNE : gene: "F7H19.10"; product: "putative protein"; Arabidopsis thaliana DNA chromosome 4, BAC clone F7H19 (ESSAII project) >TREMBL: ATT12H17_21 gene: "T12H17.210"; product: "predicted protein"; Arabidopsis thaliana DNA chromosome 4, BAC clone T12H17 (ESSAII project) Score = 206, P = 2.1e-24, identities = 51/146, positives = 77/146 Entry PVPVPR3A_1 from database TREMBL: gene: "PVPR3"; P.vulgaris PVPR3 protein mRNA, complete eds. Score = 237, P = 4.9e-20, identities = 50/136, positives = 73/136 Entry AF062072_1 from database TREMBL: gene: "ZNF216"; product: "zinc finger protein 216"; Homo sapiens zinc finger protein 216 (ZNF216) gene, complete eds. Score = 591, P = 1.6e-57, identities = 124/215, positives = 147/215 Alert BLASTP hits for DKFZphfbr2_16f21, frame 1 TREMBL:AF062071_1 product: "zinc finger protein ZNF216"; Mus musculus zinc finger protein ZNF216 mRNA, complete eds., N = 1, Score = 590, P = 2.1e-57 TREMBLNEW:AB001773_1 gene: "pem-6"; product: "PEM-6"; Ciona savignyi pem-6 (posterior end mark 6) mRNA, complete eds., N = 1, Score = 421, P = 1.7e-39 >TREMBL:AF062071_1 product: "zinc finger protein ZNF216"; Mus musculus zinc finger protein ZNF216 mRNA, complete eds. Length = 213 HSPs: Score = 590 (88.5 bits), Expect = 2.1e-57, P = 2.1e-57 Identities = 123/213 (57%), Positives = 146/213 (68%) Query: 1 MAQETNHSQVPMLCSTGCGFYGNPRTNGMCSVCYKEHLQRQNSSNGRISPPAT SVSS 57 MAQETN + PMLCSTGCGFYGNPRTNGMCSVCYKEHLQRQ +S GR+SP T S S Sb.ct: 1 MAQETNQTPGPMLCSTGCGFYGNPRTNGMCSVCYKEHLQRQQNS-GRMSPMGTASGSNSP 59 Query: 58 LSESLPVQCTDGSVPEAQSALDSTSSSMQPSPVSNQSLLSE—SVASSQLDSTSVDKAVP 115 S+S VQ D + + A STS + PV+ + + ++ S+ D + K Sb]Ct: 60 TSDSASVQRADAGLNNCEGAAGSTSEKSRNVPVAALPVTQQMTEMSISREDKITTPKT-E 118 Query: 116 ETEDVQASVSDTAQQPSEEQS—KPLEKPKQKKNRCFMCRKKVGLTGFECRCGNVYCGVH 173 +E V S + QPS QS K E PK KKNRCFMCRKKVGLTGF+CRCGN++CG+H Sb.ct: 119 VSEPVVTQPSPSVSQPSSSQSEEKAPELPKPKKNRCFMCRKKVGLTGFDCRCGNLFCGLH 178 Query: 174 RYSDVLNCSYNYKADAAEKIRKENPVVVGEKIQKI 208 RYSD NC Y+YKA+AA KIRKENPVVV EKIQ+I Sb 3 ct: 179 RYSDKHNCPYDYKAEAAAKIRKENPVVVAEKIQRI 213 Pedant information for DKFZphfbr2_16 21, frame 1 Report for DKFZphfbr2_16 21.1 [LENGTH] 208 [MW] 22541.23 [pi] 6.80 [HOMOL] TREMBL :AF062072_1 gene: "ZNF216"; product: "zinc finger protein 216"; Homo sapiens zinc finger protein 216 (ZNF216) gene, complete eds. 9e-57 [PIRKW] zinc 8e-13 [PIRKW] zinc finger 8e-13 [PIRKW] fusion protein 8e-13 [SUPFAM] unassigned ubiquitin-related proteins 8e-13 [SUPFAM] ubiquitin homology 8e-13 [PROSITE] MYRISTYL 2 [PROSITE] CK2_PHOSPHO_SITE 7 [PROSITE] ASN_GLYCOSYLATION 4 [KW] Irregular [KW] LOW_COMPLEXITY 7.21 % SEQ MAQETNHSQVPMLCSTGCGFYGNPRTNGMCSVCYKEHLQRQNSSNGRISPPATSVSSLSE SEG PRD ccccccccccccccccccccccccccccccchhhhhhhhhhccccccccccccccccccc SEQ SLPVQCTDGSVPEAQSALDSTSSSMQPSPVSNQSLLSESVASSQLDSTSVDKAVPETEDV SEG xxxxxxxxxxxxxxx PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ QASVSDTAQQPSEEQSKPLEKPKQKKNRCFMCRKKVGLTGFECRCGNVYCGVHRYSDVLN SEG PRD cccccccccccccccccccccccccccceeecccccccceeecccccccccccccccccc SEQ CSYNYKADAAEKIRKENPVVVGEKIQKI SEG PRD ccchhhhhhhhhhhhhcccccccccccc Prosite for DKFZphfbr2_16f21.1 PS00001 6->10 ASN_GLYCOSYLATION PDOC00001 PS00001 42->46 ASN_GLYCOSYLATION PDOC00001 PS00001 92->96 ASN_GLYCOSYLATION PDOC00001 PS00001 180->184 ASN_GLYCOSYLATION PDOC00001 PS00006 57->61 CK2_PHOSPHO_SITE PDOC00006 PS00006 70->74 CK2_PHOSPHO_SITE PDOC00006 PS00006 76->80 CK2_PHOSPHO_SITE PDOC00006 PS00006 103->107 CK2_PHOSPHO_SITE PDOC00006 PS00006 108->112 CK2_PHOSPHO_SITE PDOC00006 PS00006 123->127 CK2_PHOSPHO_SITE PDOC00006 PS00006 159->163 CK2_PHOSPHO_SITE PDOC00006 PS00008 22->28 MYRISTYL PDOC00008 PS00008 166->172 MYRISTYL PDOC00008 (No Pfam data available for DKFZphfbr2_16f21.1) DKFZphfbr2_16gl8 group: cell cycle DKFZphfbr2_16gl8.3 encodes a novel 984 amino acid protein with similarity to centromeric proteins of yeasts. The novel protein shows similarity to S. pombe SPAC17A5.07c and the S. cerevisiae Smt4p suppressor of MIF2 gene. MIF2 encodes a centromeric protein with homology to the mammalian centromeric protein CENP-C. Mutations in MIF2 stabilise dicentric rainichromosomes and confer high instability to chromosomes that bear a cis-acting mutation in element I of the yeast centromeric DNA (CDEI). Therefore the new protein should be involved in centromer organisation, too. The new protein can find application in modulating/blocking the cell cycle and influencing the behavior of chromosomes, both natural and artificial in eukaryotic cells. similarity to KIAA0797 and yeast Smt4p complete cDNA, complete eds, EST hits the yeast Smt4 protein seems to be involved in centromer function and microtuble organisation Sequenced by Qiagen Locus: unknown Insert length: 4826 bp Poly A stretch at pos. 4756, polyadenylation signal at pos. 4736 1 GGGTCGAGGT CGACGGTATC GATAAGTTTT TTTTTTTTTT TTTTTTTTTT 51 TTTTCCTTTC CCCTCCCCCT CCCTCTCCAA GCCGGAGGGG TCCTGAGGTG 101 ACAGCGCCTG CAACTGAAAT TTCAGCAGCG GGAGAAGATG GACAAGAGAA 151 AGCTCGGGCG ACGGCCATCT TCATCCGAAA TCATCACAGA AGGAAAAAGG 201 AAAAAGTCAT CTTCTGATTT ATCGGAGATA AGAAAGATGT TAAATGCAAA 251 ACCAGAGGAT GTCCATGTTC AATCACCACT GTCCAAATTC AGAAGCTCAG 301 AACGCTGGAC TCTCCCTTTG CAGTGGGAAA GAAGCCTAAG GAATAAAGTC 351 ATCTCTCTAG ACCATAAAAA TAAAAAACAT ATCCGAGGGT GTCCTGTTAC 401 TTCCAGGTCA TCACCAGAAA GGATACCCAG AGTTATATTG ACGAATGTCC 451 TGGGAACGGA GTTAGGAAGA AAATACATAA GGACCCCACC TGTAACTGAG 501 GGAAGTTTGA GTGATACAGA CAACTTGCAA TCAGAGCAAC TTTCTTCATC 551 ATCTGATGGC AGCCTAGAAT CTTATCAAAA TCTAAACCCT CACAAGAGCT 601 GTTATTTATC TGAAAGGGGC TCACAACGAA GTAAGACAGT AGATGACAAT 651 TCTGCAAAGC AGACTGCGCA CAATAAAGAA AAACGAAGAA AGGATGATGG 701 CATTTCTCTT TTAATATCTG ATACTCAGCC TGAAGACCTT AACAGTGGAA 751 GTAGAGGTTG TGATCATCTC GAACAGGAAA GCAGAAACAA GGATGTTAAA 801 TATTCTGATT CAAAAGTGGA ACTCACTCTG ATTTCCAGGA AGACAAAGAG 851 AAGGCTTAGA AATAATTTAC CTGATTCTCA ATATTGTACT TCTTTGGATA 901 AGTCAACAGA ACAGACAAAA AAACAAGAAG ATGACTCAAC AATATCCACT 951 GAGTTTGAAA GGCCAAGTGA AAACTATCAT CAGGATCCAA AACTGCCTGA 1001 AGAAATTACA ACTAAACCTA CAAAAAGTGA TTTTACTAAG CTATCCTCAC 1051 TTAACAGTCA GGAGTTGACT TTGAGTAATG CCACCAAAAG TGCCTCTGCC 1101 GGTTCAACCA CTGAAACCGT TGAGTACTCT AATTCCATTG ATATTGTGGG 1151 GATTTCTTCC CTGGTTGAGA AGGATGAGAA TGAGTTGAAT ACCATAGAAA 1201 AGCCTATTCT AAGAGGACAT AATGAAGGGA ACCAATCACT GATCTCAGCT 1251 GAACCAATTG TTGTTTCCAG TGATGAAGAA GGACCTGTTG AACATAAAAG 1301 TTCAGAAATT CTTAAGTTAC AATCTAAGCA AGACCGTGAG ACAACTAATG 1351 AAAATGAGAG TACTTCTGAA TCAGCATTGT TAGAACTACC ATTGATTACA 1401 TGTGAATCTG TACAGATGTC ATCTGAATTA TGCCCATATA ATCCTGTCAT 1451 GGAGAACATT TCCAGTATTA TGCCTAGTAA TGAGATGGAT CTACAACTGG 1501 ATTTTATATT TACTTCTGTT TATATTGGTA AAATAAAAGG AGCTTCTAAA 1551 GGTTGTGTTA CAATCACAAA AAAATATATT AAGATCCCAT TTCAAGTGTC 1601 CCTGAATGAG ATTTCATTGC TAGTGGATAC CACACATTTA AAGCGGTTTG 1651 GGTTATGGAA AAGTAAGGAT GATAATCACA GTAAAAGGAG TCATGCTATT 1701 CTTTTCTTCT GGGTCTCTTC AGATTATCTT CAAGAGATTC AGACCCAATT 1751 AGAACACTCT GTATTAAGCC AGCAATCAAA ATCTAGTGAA TTCATTTTCC 1801 TTGAACTACA CAATCCTGTT TCACAGAGAG AAGAATTGAA GCTGAAAGAT 1851 ATTATGACGG AAATAAGTAT AATCAGTGGA GAATTAGAGC TTTCTTACCC 1901 GTTGTCTTGG GTTCAGGCAT TTCCTTTGTT TCAGAACCTC TCTTCAAAAG 1951 AAAGTTCTTT TATTCATTAT TACTGTGTTT CAACTTGTTC TTTCCCTGCT 2001 GGTGTTGCTG TTGCTGAAGA AATGAAGCTG AAATCAGTAT CTCAGCCCTC 2051 AAACACAGAT GCGGCCAAGC CTACTTACAC CTTCCTGCAG AAGCAAAGTA 2101 GCGGTTGCTA CTCCCTTTCT ATTACATCTA ATCCAGATGA AGAATGGCGG 2151 GAAGTCAGGC ACACTGGACT TGTTCAGAAG TTGATTGTAT ATCCTCCACC 2201 ACCTACTAAG GGGGGATTGG GAGTAACTAA TGAAGATCTG GAGTGTTTAG 2251 AAGAAGGAGA GTTTCTTAAT GATGTAATCA TTGATTTTTA CCTTAAGTAT 2301 CTTATATTGG AGAAGGCATC AGATGAACTT GTTGAACGAA GTCACATTTT 2351 TAGTAGCTTT TTCTATAAAT GCTTGACAAG AAAGGAAAAT AATTTAACAG 2401 AAGATAATCC AAATCTTTCA ATGGCACAGA GAAGACATAA AAGAGTAAGA 2451 ACATGGACTC GTCACATAAA CATTTTTAAT AAAGATTACA TCTTTGTACC 2501 TGTAAATGAG TCGTCTCACT GGTATCTCGC AGTCATTTGT TTTCCATGGT 2551 TAGAAGAAGC TGTGTATGAA GATTTTCCAC AAACTGTATC CCAGCAGTCC 2601 CAGGCTCAGC AGTCCCAAAG TGACAACAAA ACAATAGATA ATGATCTACG 2651 TACTACTTCG ACACTGTCTT TGAGTGCAGA GGATTCCCAA AGTACCGAGT 2701 CGAATATGTC AGTACCAAAG AAAATGTGTA AAAGGCCATG TATTCTTATA 2751 CTAGACTCCT TGAAAGCTGC TTCTGTACGA AACACAGTTC AGAATTTACG 2801 AGAGTATTTA GAGGTAGAGT GGGAAGTTAA ACTAAAAACT CATCGTCAAT 2851 TCAGCAAAAC AAACATGGTG GATCTATGCC CTAAAGTTCC TAAACAGGAC 2901 AATAGCAGTG ATTGTGGAGT ATATTTATTG CAGTATGTGG AAAGCTTCTT 2951 CAAGGATCCT ATTGTTAACT TTGAACTTCC AATTCATTTG GAGAAGTGGT 3001 TTCCTCGTCA TGTAATAAAG ACCAAACGGG AAGATATTCG AGAGCTCATC 3051 TTGAAACTTC ATTTACAGCA ACAGAAGGGC AGCAGTAGCT AGTTAATCTG 3101 TACAAACATG ACACAGATGT TCTCTAAGAT TACTGGAAAG CCCCTTACCA 3151 GCATTTGTGT TAGCCAGCTC ACAGAGAAGA AAATAACTTG CAGTAGTTTT 3201 ATAATAAGTC ATTGGAACAT TATTTAAAAT ATGTAGGACA CATTATTAGA 3251 ATTGTTGGGA TCTCATAGAT GGAATGGGAA TGGGGGTGAT ATAGATAAAC 3301 TTACTAGATA TAAATTAAAA TTTTATAAAT ATTTCATATT TTTCTGAGTA 3351 AATATGATTG GATTATGCAA CAGCATATGT AATATGGGAA TGTTTTGTAG 3401 ATAATAAAAC TTACATGATC TGTACTTCCA CGTGACTGGG TGCTGAGGGG 3451 AGTTAAAGCC TCCCTGGTGC CAGCCCCAGT GCTTGTCAAA TTTGCTGACA 3501 GGTCACATCA TATTGTAATT CTATTCTTTG CAGCTCAAGC ATGCAGTATG 3551 AATACTGTGT ATTTTTTAAA AAAATAATTT AGTATCAAGG CTTCAGAAAA 3601 TGCCATTTAC GGCATCCCTT CTGTATGTAA CAAAAAGACA TTCATAATGT 3651 TAGGAAGATG ATAAAAATTC GCTCTTTTAA AGTGCAGCTT ATTATTCTCA 3701 ATTGCTAAAT ACGATTACTC TGCTTTTTTT TTTTCATTTC TTTTGATGTC 3751 ATATGTGAGT ATCTTATAAT TTAGTTCATT TGTTCAGGGT AAAATTTGAA 3801 ACAAAAAATT TTACCTGTGC AAAATAGTTT TTTAAAAATT ATACATGTAG 3851 CTCAACTTGA GGTACTGCTA TATAAATATT CACTCACATT ATCACGGAAT 3901 TTATGTATAG TTTCTCTAAT ATAGAAGATA AAATTGGTGT CCTCATAACT 3951 TTAACAAAGA AAACCCTCAG TCCTATTTAT TAATGGGTAG AATTAAATAT 4001 ATAATTTTAT AGCTCAGTTT ACCCAGTATT CATCTGCAAA GCCAGATTGC 4051 TCTCATTGCT TTTATATTTT TAAATTGTAG CTTTTAGAGA CCTATGATCC 4101 TCATGGAACT TAATTTTTTA TTAAATATTC AGGTAACAGT TCTGAATTCA 4151 TGTGATAATG GTGGCATTAT ATATGATTAA ACACTTCAGA ACTTTCTAAT 4201 GTTATCAGGA GTATTTTGAG GGAGATATGA TTATATTGTA TTTTCTCAGA 4251 TAAGAAAAAT GTTTTTTAAC AATATTATTT TAATCTGTTT TAAGCATCTC 4301 TTAGATTTAC ATTATAACTA CATAAAGCAG TGAAGCAAAG GCAAATTAAG 4351 ATAAAGCTAG AAAGTCTGAA CATTTTATTT CAAAATCATA CGAATCGGGG 4401 TCAGTTAAGC CTCAGTATTC TTAGCTTTTG TTGATTTTGG CACTATCTTT 4451 ATATTATTAA ATATATTTGT TGTTTGGATA TTTCATATAA AGATGGCTAT 4501 AATTACATAT TTCATTCCCA ATTTGTGTGT GTTGGGGGGT ACTTTTAAAG 4551 GTGACTATTG TTTTGTACAT CTAATTTTGG GAAACCAAGT CTATAAGACA 4601 TCTTGTGATT TCTTAATGTT TTTGTTTGTA TGTTTTTCAA AGATA CACT 4651 GTCCTTTATC ATGTTTTGAA GATTGTTTAA AATTCATTTT CCTAAATTAA 4701 TGTGCAAGTA ATGTTTTGAG GATATCGGTG TTTTATATTA AACATATTTC 4751 CAATTCAAAA AAAAAAAAAA AAAAACTTAT CGATACCGTC GACCTCGATG 4801 ATGATGATGA TGATGATGAT GTCGAC BLAST Results No BLAST result Medline entries No Medlme entry Peptide information for frame 3 ORF from 138 bp to 3089 bp; peptide length: 984 Category: similarity to known protein 1 MDKRKLGRRP SSSEIITEGK RKKΞSSDLSE IRKMLNAKPE DVHVQSPLΞK 51 FRSSERWTLP LQWERSLRNK VISLDHKNKK HIRGCPVTSR SSPERIPRVI 101 LTNVLGTELG RKYIRTPPVT EGSLSDTDNL QSEQLSSSSD GSLESYQNLN 151 PHKSCYLSER GSQRSKTVDD NSAKQTAHNK EKRRKDDGIΞ LLISDTQPED 201 LNSGSRGCDH LEQESRNKDV KYSDSKVELT LISRKTKRRL RNNLPDSQYC 251 TSLDKSTEQT KKQEDDSTIS TEFERPSENY HQDPKLPEEI TTKPTKSDFT 301 KLSSLNSQEL TLSNATKSAS AGSTTETVEY ΞNSIDIVGIS SLVEKDENEL 351 NTIEKPILRG HNEGNQSLIS AEPIVVSSDE EGPVEHKSSE ILKLQΞKQDR 401 ETTNENESTS ESALLELPLI TCESVQMSSE LCPYNPVMEN ISSIMPSNEM 451 DLQLDFIFTS VYIGKIKGAS KGCVTITKKY IKIPFQVSLN EISLLVDTTH 501 LKRFGLWKSK DDNHSKRSHA ILFFWVSΞDY LQEIQTQLEH SVLSQQSKSS 551 EFIFLELHNP VSQREELKLK DIMTEISIIS GELELSYPLS WVQAFPLFQN 601 LSSKESSFIH YYCVSTCSFP AGVAVAEEMK LKSVSQPSNT DAAKPTYTFL 651 QKQSSGCYSL SITSNPDEEW REVRHTGLVQ KLIVYPPPPT KGGLGVTNED 701 LECLEEGEFL NDVIIDFYLK YLILEKASDE LVERSHIFSS FFYKCLTRKE 751 NNLTEDNPNL SMAQRRHKRV RTWTRHINIF NKDYIFVPVN ESSHWYLAVI 801 CFPWLEEAVY EDFPQTVSQQ ΞQAQQSQSDN KTIDNDLRTT STLSLSAEDS 851 QSTESNMSVP KKMCKRPCIL ILDSLKAASV RNTVQNLREY LEVEWEVKLK 901 THRQFSKTNM VDLCPKVPKQ DNSSDCGVYL LQYVESFFKD PIVNFELPIH 951 LEK FPRHVI KTKREDIREL ILKLHLQQQK GSSS BLASTP hits Entry SPAC17A5_7 from database TREMBL: "ΞPAC17A5.07c"; product: "hypothetical protein"; S. pombe chromosome I cosmid cl7A5. Schizosaccharomyces pombe (fission yeast) Length = 652 Score = 275 (96.8 bits). Expect = 1.9e-29, Sum P(3) = 1.9e-29 Identities = 56/120 (46%), Positives = 78/120 (65%) Entry S49947 from database PIR: SMT4 protein - yeast (Ξaccharomyces cerevisiae) Length = 1034 Score = 163 (57.4 bits), Expect = 4.6e-16, Sum P(3) = 4.6e-16 Identities = 46/159 (28%), Positives = 76/159 (47%) Entry YQG6_CAEEL from database SWISSPROT: HYPOTHETICAL 35.7 KD PROTEIN C41C4.6 IN CHROMOSOME II. Length = 342 Score = 162 (57.0 bits), Expect = 6.1e-13, Sum P(3) = 6.1e-13 Identities = 37/119 (31%), Positives = 62/119 (52%) Entry AB018340_1 from database TREMBL: gene: "KIAA0797"; product: "KIAA0797 protein"; Homo sapiens mRNA for KIAA0797 protein, partial eds. Score = 540, P = 1.9e-50, identities = 120/243, positives = 155/243 Alert BLASTP hits for DKFZphfbr2_16gl8, frame 3 TREMBL :ATT16L1_11 gene: "T16L1.110"; product: "putative protein"; Arabidopsis thaliana DNA chromosome 4, BAC clone T16L1 (ESSAII project), N = 2, Score = 239, P = 2.1e-18 >TREMBL:ATT16L1_11 gene: "T16L1.110"; product: "putative protein"; Arabidopsis thaliana DNA chromosome 4, BAC clone T16L1 (ESSAII project) Length = 710 HSPs: Score = 239 (35.9 bits), Expect = 2.1e-18, Sum P(2) = 2.1e-18 Identities = 51/135 (37%), Positives = 78/135 (57%) Query: 683 IVYPPPPTKGGLGVTNEDLECLEEGEFLNDVIIDFYLKYLILEKASDELVERSHIFSSFF 742 +VYP + V +D+E L+ F+ND IIDFY+KYL + S + R H F+ FF Ξbjct: 176 LVYPQGEPDAVV-VRKQDIELLKPRRFINDTIIDFYIKYL-KNRISPKERGRFHFFNCFF 233 Query: 743 YKCLTRKENNLTEDNPNLSMAQRRHKRVRTWTRHINIFNKDYIFVPVNESSHWYLAVICF 802 + RK NL + P+ + ++RV+ WT+++++F KDYIF+P+N S HW L +IC Sb ct: 234 F RKLANLDKGTPSTCGGREAYQRVQKWTKNVDLFEKDYIFIPINCSFHWSLVIICH 289 Query: 803 PWLEEAVYEDFPQTV 817 P + + PQ V Sbjct: 290 PGELVPSHVENPQRV 304 Score = 70 (10.5 bits), Expect = 2.1e-18, Sum P(2) = 2.1e-18 Identities = 13/28 (46%), Positives = 15/28 (53%) Query: 948 PIHLEKWFPRHVIKTKREDIRELILKLH 975 P HL FP KR +1 EL+ LH Sbjct: 403 PSHLRNWFPAKEASLKRRNILELLYNLH 430 Pedant information for DKFZphfbr2_16gl8, frame 3 Report for DKFZphfbr2_16gl8.3 [LENGTH] 984 [MW] 112265.80 [pi] 6.13 [HOMOL] TREMBL:AB018340_1 gene: "KIAA0797"; product: "KIAA0797 protein"; Homo sapiens mRNA for KIAA0797 protein, partial eds. 8e-53 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YIL031w] 9e-17 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YPL020c] 4e-06 [BLOCKS] BL00494C Bacterial luciferase subunits proteins [PROSITE] AMIDATION 3 [PROSITE] MYRISTYL 9 [PROSITE] CAMP PH0SPH0 SITE 2 [PROSITE] CK2 PHOSPHO SITE 30 [PROSITE] TYR PHOSPHO SITE 1 [PROSITE] PKC PHOSPHO SITE 19 [PROSITE] ASN GLYCOSYLATION 12 [KW] Alpha Beta [KW] LOW COMPLEXITY 4.47 % SEQ MDKRKLGRRPSSSEIITEGKRKKSSSDLSEIRKMLNAKPEDVHVQSPLSKFRSSERWTLP SEG PRD ccccceeecccceeeeecccccccccchhhhhhhhhhccccccccccccccccccccchh SEQ LQWERSLRNKVISLDHKNKKHIRGCPVTSRΞSPERIPRVILTNVLGTELGRKYIRTPPVT SEG PRD hhhhhhhhhheeeeccccceeeccccccccccccceeeeeeeeeccceeeccceeecccc SEQ EGSLSDTDNLQSEQLSSSSDGSLESYQNLNPHKSCYLSERGSQRSKTVDDNSAKQTAHNK SEG xxxxxxxxxxxxxxxx PRD cccccccccccccccccccccccccccccccccccccccccccccccccccchhhhhhhh SEQ EKRRKDDGISLLISDTQPEDLNSGSRGCDHLEQESRNKDVKYSDΞKVELTLISRKTKRRL SEG PRD hhhhcccceeeeecccccccccccccccccccccccccccccccccceeeeeehhhhhhh SEQ RNNLPDSQYCTSLDKSTEQTKKQEDDSTISTEFERPSENYHQDPKLPEEITTKPTKSDFT SEG PRD hccccccccccccccccchhhhhccccccccccccccccccccccccccccccccccccc SEQ KLSSLNSQELTLSNATKSASAGSTTETVEYSNSIDIVGISSLVEKDENELNTIEKPILRG SEG PRD ccccccccceeehhhhhhhcccccceeeeccceeeceeeccchhhhhhhhhhhccccccc SEQ HNEGNQSLISAEPIVVSSDEEGPVEHKSSEILKLQSKQDRETTNENESTSESALLELPLI SEG xxxxxxxxxxxxxxxxx ... PRD cccccceeeecceeeeecccccccccchhhhhhhhhhhhhhcccccccchhhhhccccce SEQ TCESVQMSSELCPYNPVMENISΞIMPSNEMDLQLDFIFTSVYIGKIKGASKGCVTITKKY SEG PRD eecccccccccccccccccceeeccccchhhhhhheeeeeeeeeeeeccccceeeeeeee SEQ IKIPFQVSLNEISLLVDTTHLKRFGLWKSKDDNHSKRSHAILFFWVSSDYLQEIQTQLEH SEG PRD eeeeccccceeeeeeecccceeeeeeeecccccccccceeeeeeeeccchhhhhhhhhhh SEQ SVLSQQSKSSEFIFLELHNPVSQREELKLKDIMTEISIISGELELSYPLSWVQAFPLFQN SEG PRD hhhhccccceeeeeeeeccccccchhhhhhhhhheeeeeccceeeeccceeeeeeceeec SEQ LSSKESSFIHYYCVSTCSFPAGVAVAEEMKLKSVSQPSNTDAAKPTYTFLQKQSSGCYSL SEG PRD ccccccccceeeeecccccccchhhhhhhhhhhcccccccccccccceeeecccccccce SEQ SITSNPDEEWREVRHTGLVQKLIVYPPPPTKGGLGVTNEDLECLEEGEFLNDVIIDFYLK SEG : PRD eeccccccceeeeeeccceeeeeeecccccccccccccchhhhhhhhccchhhhhhhhhh SEQ YLILEKASDELVERSHIFSSFFYKCLTRKENNLTEDNPNLSMAQRRHKRVRTWTRHINIF SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccccccchhhhhhhhhhhhhhhhhhhhc SEQ NKDYIFVPVNESSHWYLAVICFPWLEEAVYEDFPQTVSQQSQAQQSQSDNKTIDNDLRTT SEG xxxxxxxxxxx PRD cceeeeeccccccceeeeeeeccchhhhhhhccccchhhhhhhhhhcccccccccccccc SEQ STLSLSAEDSQSTEΞNMSVPKKMCKRPCILILDSLKAASVRNTVQNLREYLEVEWEVKLK SEG PRD cceeeeecccccceeeccccccccccceeeeeccccccccchhhhhhhhhhhhhhhhhhh SEQ THRQFSKTNMVDLCPKVPKQDNSSDCGVYLLQYVESFFKDPIVNFELPIHLEKWFPRHVI SEG PRD hhhhhccccccccccccccccccccceeeeehhhhhhhcccceeecccccccccccchhh SEQ KTKREDIRELILKLHLQQQKGSSS SEG PRD hhhhhhhhhhhhhhhhhhhccccc Prosite for DKFZphfbr2_16gl8.3 PS00001 314->318 ASN_GLYCOSYLATION PDOC00001 PS00001 365->369 ASNJΞLYCOSYLATION PDOC00001 PS00001 406->410 ASN_GL COSYLATION PDOC00001 PS00001 440->444 ASN_GLYCOSYLATION PDOC00001 PΞ00001 513->517 ASN_GLYCOSYLATION PDOC00001 PS00001 600->604 ASN_GLYCOSYLATION PDOC00001 PS00001 752->756 ASN_GLYCOSYLATION PDOC00001 PS00001 759->763 ASN_GLYCOSYLATION PDOC00001 PS00001 790->794 ASN_GLYCOSYLATION PDOC00001 PS00001 830->834 ASN_GLYCOSYLATION PDOC00001 PSOOOOl 856->860 ASN_GLYCOSYLATION PDOC00001 PS00001 922->926 ASN 3LYCOSYLATION PDOC00001 PS00004 8->12 CAMP_PHOSPHO_SITE PDOC00004 PS00004 21->25 CAMP_PHOSPHO_SITE PDOC00004 PS00005 54->57 PKC_PHOSPHO_SITE PDOC00005 PS00005 66->69 PKC_PHOSPHO_SITE PDOC00005 PS00005 88->91 PKC_PHOSPHO_SITE PDOC00005 PS00005 158->161 PKC_PHOSPHO_SITE PDOC00005 PS00005 162->165 PKC_PHOSPHO_SITE PDOC00005 PS00005 172->175 PKC_PHOSPHO_SITE PDOC00005 PS00005 233->236 PKC_PHOSPHO_SITE PDOC00005 PS00005 236->239 PKC_PHOSPHO_SITE PDOC00005 PS00005 260->263 PKC_PHOSPHO_SITE PDOC00005 PS00005 291->294 PKC_PHOSPHO_SITE PDOC00005 PS00005 477->480 PKC_PHOSPHO_SITE PDOC00005 PS00005 515->518 PKC_PHOSPHO_ΞITE PDOC00005 PS00005 562->565 PKC_PHOSPHO_SITE PDOC00005 PS00005 602->605 PKC_PHOSPHO_SITE PDOC00005 PS00005 747->750 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 874->877 PKC_PHOSPHO_SITE PDOC00005 PS00005 879->882 PKC_PHOSPHO_SITE PDOC00005 PS00005 901->904 PKC_PHOSPHO_SITE PDOC00005 PS00005 962->965 PKC_PHOSPHO_SITE PDOC00005 PS00006 11->15 CK2_PHOSPHO_SITE PDOC00006 PS00006 24->28 CK2_PHOSPHO_SITE PDOC00006 PS00006 91->95 CK2_PHOSPHO_SITE PDOC00006 PS00006 123-M27 CK2_PHOSPHO_SITE PDOC00006 PS00006 125->129 CK2_PHOSPHO_SITE PDOC00006 PS00006 137->141 CK2_PHOSPHO_SITE PDOC00006 PS00006 167->171 CK2_PHOSPHO_SITE PDOC00006 PS00006 196->200 CK2_PHOSPHO_SITE PDOC00006 PS00006 225->229 CK2_PHOSPHO_SITE PDOC00006 PS00006 251->255 CK2_PHOSPHO_SITE PDOC00006 PS00006 271->275 CK2_PHOΞPHO_SITE PDOC00006 PS00006 295-> 2 99 CK2_PHOSPHO_SITE PDOC00006 PΞ00006 323->327 CK2_PHOSPHO_SITE PDOC00006 PS00006 341->345 CK2_PHOSPHO_SITE PDOC00006 PS00006 377->381 CK2_PHOSPHO_SITE PDOC00006 PS00006 396->400 CK2_PHOSPHO_SITE PDOC00006 PS00006 402->406 CK2_PHOSPHO_SITE PDOC00006 PS00006 408->412 CK2_PHOSPHO_SITE PDOC00006 PS00006 488->492 CK2_PHOSPHO_SITE PDOC00006 PS00006 509->513 CK2_PHOSPHO_SITE PDOC00006 PΞ00006 536->540 CK2_PHOSPHO_SITE PDOC00006 PS00006 562->566 CK2_PHOSPHO_SITE PDOC00006 PΞ00006 602->606 CK2_PHOSPHO_SITE PDOC00006 PS00006 638->642 CK2_PHOSPHO_SITE PDOC00006 PS00006 664->668 CK2_PHOSPHO_SITE PDOC00006 PS00006 697->701 CK2_PHOSPHO_SITE PDOC00006 PS00006 747->751 CK2_PHOSPHO_SITE PDOC00006 PS00006 826->830 CK2_PHOSPHO_SITE PDOC00006 PS00006 846->850 CK2_PHOSPHO_SITE PDOC00006 PS00006 962->966 CK2_PHOSPHO_SITE PDOC00006 PS00007 216->223 TYR_PHOSPHO_SITE PDOC00007 PS00008 84->90 MYRISTYL PDOC00008 PS00008 106-M12 MYRISTYL PDOC00008 PS00008 141->147 MYRISTYL PDOC00008 PS00008 161-M67 MYRISTYL PDOC00008 PS00008 204->210 MYRISTYL PDOC00008 PS00008 468->474 MYRISTYL PDOC00008 PS00008 505->511 MYRISTYL PDOC00008 PS00008 622->628 MYRISTYL PDOC00008 PS00008 693->699 MYRISTYL PDOC00008 PS00009 6->10 AMIDATION PDOC00009 PS00009 18->22 AMIDATION PDOC00009 PS00009 109->113 AMIDATION PDOC00009 (No Pfam data available for DKFZphfbr2_16gl8.3) DKFZphfbr2_16ιl2 group: transmembrane protein DKFZphfbr2_16ιl2 encodes a novel 185 amino acid protein, with strong similarity to PUT2 protein of Fugu rubripes. The novel protein contains 1 transmembrane region. PUT 2 is a Fugu rupies protein similar to the neural cell adhesion molecule LI (Ll-CAM) a mitosis-specific chromosome segregation protein (SMCl) and the calcium channel alpha-1 subunit homolog (CCA1) . No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of bram-specific genes and as a new marker for neuronal cells. strong similarity to Fugu rubripes PUT2 complete cDNA, complete eds, EST hits, TRANSMEMBRANE 1 Sequenced by LMU Locus: /map="873.3/875.1 CR from top of Chrl linkage group" Insert length: 1552 bp Poly A stretch at pos. 1528, polyadenylation signal at pos. 1506 1 GGGGGGGGAC AACTGGGTCT TTTGCGGCTG CAGCGGGCTT GTAGGCGTCC 51 GGCTTTGCTG GCCCAGCAAG CCTGATAAGC ATGAAGCTCT TATCTTTGGT 101 GGCTGTGGTC GGGTGTTTGC TGGTGCCCCC AGCTGAAGCC AACAAGAGTT 151 CTGAAGATAT CCGGTGCAAA TGCATCTGTC CACCTTATAG AAACATCAGT 201 GGGCACATTT ACAACCAGAA TGTATCCCAG AAGGACTGTT GTAGCAACTG 251 CCTGCACGTG GTGGAGCCCA TGCCAGTGCC TGGCCATGAC GTGGAGGCCT 301 ACTGCCTGCT GTGCGAGTGC AGGTACGAGG AGCGCAGCAC CACCACCATC 351 AAGGTCATCA TTGTCATCTA CCTGTCCGTG GTGGGTGCCC TGTTGCTCTA 401 CATGGCCTTC CTGATGCTGG TGGACCCTCT GATCCGAAAG CCGGATGCAT 451 ACACTGAGCA ACTGCACAAT GAGGAGGAGA ATGAGGATGC TCGCTCTATG 501 GCAGCAGCTG CTGCATCCCT CGGGGGACCC CGAGCAAACA CAGTCCTGGA 551 GCGTGTGGAA GGTGCCCAGC AGCGGTGGAA GCTGCAGGTG CAGGAGCAGC 601 GGAAGACAGT CTTCGATCGG CACAAGATGC TCAGCTAGAT GGGCTGGTGT 651 GGTTGGGTCA AGGCCCCAAC ACCATGGCTG CCAGCTTCCA GGCTGGACAA 701 AGCAGGGGGC TACTTCTCCC TTCCCTCGGT TCCAGTCTTC CCTTTAAAAG 751 CCTGTGGCAT TTTTCCTCCT TCTCCCTAAC TTTAGAAATG TTGTACTTGG 801 CTATTTTGAT TAGGGAAGAG GGATGTGGTC TCTGATCTCT GTTGTCTTCT 851 TGGGTCTTTG GGGTTGAAGG GAGGGGGAAG GCAGGCCAGA AGGGAATGGA 901 GACATTCGAG GCGGCCTCAG GAGTGGATGC GATCTGTCTC TCCTGGCTCC 951 ACTCTTGCCG CCTTCCAGCT CTGAGTCTTG GGAATGTTGT TACCCTTGGA 1001 AGATAAAGCT GGGTCTTCAG GAACTCAGTG TTTGGGAGGA AAGCATGGCC 1051 CAGCATTCAG CATGTGTTCC TTTCTGCAGT GGTTCTTATC ACCACCTCCC 1101 TCCCAGCCCC AGCGCCTCAG CCCCAGCCCC AGCTCCAGCC CTGAGGACAG 1151 CTCTGATGGG AGAGCTGGGC CCCCTGAGCC CACTGGGTCT TCAGGGTGCA 1201 CTGGAAGCTG GTGTTCGCTG TCCCCTGTGC ACTTCTCGCA CTGGGGCATG 1251 GAGTGCCCAT GCATACTCTG CTGCCGGTCC CCTCACCTGC ACTTGAGGGG 1301 TCTGGGCAGT CCCTCCTCTC CCCAGTGTCC ACAGTCACTG AGCCAGACGG 1351 TCGGTTGGAA CATGAGACTC GAGGCTGAGC GTGGATCTGA ACACCACAGC 1401 CCCTGTACTT GGGTTGCCTC TTGTCCCTGA ACTTCGTTGT ACCAGTGCAT 1451 GGAGAGAAAA TTTTGTCCTC TTGTCTTAGA GTTGTGTGTA AATCAAGGAA 1501 GCCATCATTA AATTGTTTTA TTTCTCTCAA AAAAAAAAAA AAAAAAAATA 1551 TC BLAST Results Entry HS808349 from database EMBL: human STS WI-11986. Score = 1716, P = 5.7e-73, identities = 364/378 Entry HS487355 from database EMBL: human STS WI-13088. Score = 1358, P = 1.3e-56, identities = 274/277 Medl e entries No Medline entry Peptide information for frame 3 ORF from 81 bp to 635 bp; peptide length: 185 Category: similarity to unknown protein 1 MKLLSLVAVV GCLLVPPAEA NKSSEDIRCK CICPPYRNIS GHIYNQNVSQ 51 KDCCSNCLHV VEPMPVPGHD VEAYCLLCEC RYEERSTTTI KVIIVIYLSV 101 VGALLLYMAF LMLVDPLIRK PDAYTEQLHN EEENEDARSM AAAAASLGGP 151 RANTVLERVE GAQQRWKLQV QEQRKTVFDR HKMLS BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_16ιl2, frame 3 TREMBL:AF026198_5 gene: "PUT2"; product: "putative protein 2"; Fugu rubripes neural cell adhesion molecule LI homolog (Ll-CAM) gene, complete eds; putative protein 1 (PUTl) gene, partial eds; mitosis-specific chromosome segregation protein SMCl homolog (SMCl) gene, complete eds; and calcium channel alpha-1 subunit homolog (CCAl) and putative protein 2 (PUT2) genes, partial eds, complete sequence., N = 1, Score = 655, P = 2.8e-64 TREMBL :CER12C12_5 gene: "R12C12.6"; Caenorhabditis elegans cosmid R12C12., N = 1, Score = 225, P = le-18 >TREMBL:AF026198_5 gene: "PUT2"; product: "putative protein 2"; Fugu rubripes neural cell adhesion molecule LI homolog (Ll-CAM) gene, complete eds; putative protein 1 (PUTl) gene, partial eds; mitosis-specific chromosome segregation protein SMCl homolog (SMCl) gene, complete eds; and calcium channel alpha-1 subunit homolog (CCAl) and putative protein 2 (PUT2) genes, partial eds, complete sequence. Length = 187 HSPs: Score = 655 (98.3 bits), Expect = 2.8e-64, P = 2.8e-64 Identities = 124/163 (76%), Positives = 140/163 (85%) Query: 22 KSSEDIRCKCICPPYRNISGHIYNQNVSQKDCCSNCLHVVEPMPVPGHDVEAYCLLCECR 81 KS +D+RCKCICPPYRNISGHIYN+N +QKDC NCLHVV+PMPVPG+DVEAYCLLCEC+ Sbjct: 31 KSFDDVRCKCICPPYRNISGHIYNRNFTQKDC—NCLHVVDPMPVPGNDVEAYCLLCECK 88 Query: 82 YEERSTTTIKVIIVIYLSVVGALLLYMAFLMLVDPLIRKPDAYTEQLHNEEENEDARSMA 141 YEERST TI+V I+I+LSVVGALLLYM FL+LVDPLIRKPD + LHNEE++ED + Sbjct: 89 YEERSTNTIRVTIIIFLSVVGALLLYMLFLLLVDPLIRKPDPLAQTLHNEEDSEDIQPQM 148 Query: 142 AAAASLGGP-RANTVLERVEGAQQRWKLQVQEQRKTVFDRHKML 184 + G P R NTVLERVEGAQQRWK QVQEQRKTVFDRHKML Sbjct: 149 S GDPARGNTVLERVEGAQQRWKKQVQEQRKTVFDRHKML 187 Pedant information for DKFZphfbr2_16ιl2, frame 3 Report for DKFZphfbr2_16ιl2.3 [LENGTH] 185 [MW] 20764.29 [pi] 6.21 [HOMOL] TREMBL:AF026198_5 gene: "PUT2"; product: "putative protein 2' Fugu rubripes neural cell adhesion molecule LI homolog (Ll-CAM) gene, complete eds; putative protein 1 (PUTl) gene, partial eds; mitosis-speeific chromosome segregation protein SMCl homolog (SMCl) gene, complete eds; and calcium channel alpha-1 subunit homolog (CCAl) and putative protein 2 (PUT2) genes, partial eds, complete sequence. 3e-68 [PROSITE] MYRISTYL 1 [PROSITE] CK2_PHOSPHO_SITE 4 [PROSITE] PKC_PHOSPHO_SITE 2 [PROSITE] ASN_GLYCOSYLATION 3 [KW] SIGNAL PEPTIDE 21 [KW] TRANSMEMBRANE 1 [KW] LOW COMPLEXITY 2.70 SEQ MKLLSLVAWGCLLVPPAEANKΞSEDIRCKCICPPYRNISGHIYNQNVSQKDCCSNCLHV SEG PRD ccceeeeeeeeccccccccccccccceeeeeecccccccccceeeccccccccccceeee MEM SEQ VEPMPVPGHDVEAYCLLCECRYEERSTTTIKVIIVI LSVVGALLLYMAFLMLVDPLIRK SEG PRD eecccccccccchhhhhhhhhhhhccccceeeeeeehhhhhhhhhhhhhhhhhhhccccc MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM ... SEQ PDAYTEQLHNEEENEDARSMAAAAASLGGPRANTVLERVEGAQQRWKLQVQEQRKTVFDR SEG xxxxx PRD ccchhhhhhhhhcccchhhhhhhhhhccccccchhhhhhhchhhhhhhhhhhhhhhhhhh MEM SEQ HKMLS SEG PRD hhccc MEM Prosite for DKFZphfbr2_16ιl2.3 PS00001 21->25 ASN_GLYCOSYLATION PDOC00001 PS00001 38->42 ASN_GLYCOSYLATION PDOC00001 PS00001 47->51 ASN_GLYCOΞYLATION PDOC00001 PS00005 49->52 PKC_PHOSPHO_ΞITE PDOC00005 PS00005 89->92 PKC_PHOSPHO_SITE PDOC00005 PS00006 23->27 CK2_PHOSPHO_SITE PDOC00006 PS00006 49->53 CK2_PHOSPHO_SITE PDOC00006 PS00006 154->158 CK2_PHOSPHO_SITE PDOC00006 PS00006 176->180 CK2_PHOSPHO_SITE PDOC00006 PS00008 148->154 MYRISTYL PDOC00008 (No Pfam data available for DKFZphfbr2 16ιl2.3) DKFZphfbr2_16k22 group: brain derived DKFZphfbr2_16k22 encodes a novel 108 amino acid protein with very weak similarity to thioredoxin of Bacillus subtilis. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of brain-specific genes . weak similarity to thioredoxin complete cDNA, complete eds, genomic DNA? Sequenced by BMFZ Locus : unknown Insert length: 2088 bp Poly A stretch at pos. 2065, no polyadenylation signal found 1 AAAAGGAAGA AGGAAATAAG GATATTTCAA GGGTTACCAA AGTCGAGGAA 51 AACTATTTTA AGAAGAAATC TGAATTATTT GTGCACATAG GTTGTAATAA 101 TAGCATCTTG CATTAAATGG TGTTTTCTAG CTTACAAAGT GGATTCATAT 151 ACACTATTGT AACTGACTCT CTACAAACTT GCAAGGTTAG CAAGACAAAT 201 GGTATTTTAA GATAACAAAC TGAGACTCAA AAAAGGCAAG TAACTCGTTC 251 TACTTCCCAA AGCCAGAAAG TGGCAAAATA GAAAATGGAT CCTGAATCTC 301 CAACACCATG CAAACTAAGA GAGGGAATCC TCTGTAGAGG GAATGGAAGT 351 AAAAAGGCAC AAGTGGTGAT GTCACCTTCT GAACAGAGAT GGAACTTTTC 401 TTCCTCTGAG AAAAAAGAGA AAAGATAGTT TTAAGTGGCA AAAGAACATG 451 AAGCAATGTG AGGTGAAGAA ACAGAAAAGA CTATGGATGG AATTCCTAGA 501 TGTGAGATAC ACAAAGTTCC ATTTCAAAGA GAAATATCTA TAGATAGGCA 551 TAAAGTTACA CACCTGAACT ACCAACTCTG AACCAGTAAC TCAAGAGATA 601 TTTTGTGTGT CCCACAAGCC ATATGGCTCT GGGGACAAAT TATCTGAAAG 651 TGCCCAATAA GAAAAATATT TGAGGAAGGG GAGTTGGTGA GTGAATGAAT 701 TAAAGGACAT CAGAAAGATA CATTGACTGT TCTCCTTCCC AGGAAACAAA 751 GTGGCTAAGT CAAAACAACG GGCAGCTGTG GGATAGCAAA GAAAAAAAAA 801 CTTCCAGGCC CAGGTTCTAG TGAAAGCTAC TATGGAAGTT AGCCACTCAA 851 CTTTAGAACC AGAGGCTTCT TTTCCTCCTC CCTTCTTATC TTTTCTAGTT 901 TATAGCAAAT TTATATTGAG CCACTTATTC TTTCTGAATG CTAGTTCCCC 951 TTTAGCATTT CTTTTTCTTC ATTCCCTTTG GACTGGCCCA ATGCTTTGGC 1001 CCCTTATCAA AGCATTTTCT AAGAAACAGT CTGACAGCTC TAATTTGCAT 1051 CTGGTTATGC AAGATGTGGT TAAGAACATG GACTCTGGAG GTAAATACAC 1101 CTTGATTCCA ATTCATTCTC TCATTTATTC ATTCAGCAAA TATTTAGTGA 1151 ACATCTAACA TGTGCTAGGC ACTGTTCTAG TTGCTGAGGA TACAGCTTCA 1201 AACAAAATAA GGTCTCTGCA AGGATGCCTT CTCTTACCAC TCCTATTCAG 1251 CGTAGTATTG GAAGTCCTGG CCAGGGCAAT CAGGCAAGAA AAAGAAATCA 1301 AGGTCATCCA AATAGGAAGA GAGGAAGTCA AACTATCCCT GTTTACAGAC 1351 AACATGATCC TACATCTAGA AAAAAACCCA TTGTCTTAGC CCAAAAGCTT 1401 CTTAGGCTGA TAAACAACTT CAGCAAAGTC TTAGGATACA AAATCCATGT 1451 GCAAAAAACA CTAGCATTCT TATACACCAA CAACAGTCAA GCCGAGATCC 1501 AAATCAGGAA CAAACTCCTA TTCACAATTG CCACAAAAAC AATAGAACAG 1551 GAAAACAGCT AACTAGGAAG GTGAAAGATC TCTACAAGGA GAACTACAAA 1601 CCACTGCTCA CAGAAATCAG AGATGACACA TATAAATGGA AAAACATTCC 1651 ATGATCATGG ATAGGAAGAA TGAATATTAC TGAAATGGCT ATACTGTCCA 1701 AAGCAATTTA TAGATTCAAT GCTATTCCTA GTAAACTACC ATTGAGATTT 1751 TTTACAGAAC TAGAAAAAAA AAAAACTATT TTAAGGCTGG GCGCAGTGGC 1801 TCTCACCTGT AATCCCAGCA CTTTGGGAGG CCGAGATGGG TGGATCACGA 1851 GGTCAGGAGA TGGAAAACAT CCTGGCTAAC ATGGTGAAAC CCCGTCTCTA 1901 CTAAAAATAC AAAAAATTAG CCAGGCGTGG TGGTGGGCGC CTGTAATCCC 1951 AGCTGCTCGG GAGGCTGAGG CAGGATAATG GTGTGAACCC GGGAGGCAGA 2001 GCTTGCAGTG AGCTGAGATT GCACCACTGC ACTCCAGCCT GAGGGACAGA 2051 GTGAGACTCC ATCTCAAAAA AAAAAAAAAA AAAAAAAA BLAST Results o BLAST result Medline entries No Medlme entry Peptide information for frame 1 ORF from 832 bp to 1155 bp; peptide length: 108 Category: putative protein 1 MEVSHSTLEP EAΞFPPPFLS FLVYSKFILΞ HLFFLNASSP LAFLFLHSLW 51 TGPMLWPLIK AFSKKQSDSS NLHLVMQDVV KNMDSGGKYT LIPIHSLIYS 101 FSKYLVNI BLASTP hits Entry B37192 from database PIR: thioredoxin - Bacillus subtilis Score = 71 (25.0 bits), Expect = 0.040, P = 0.039 Identities = 16/49 (32%), Positives = 30/49 (61%) Alert BLASTP hits for DKFZphfbr2_16k22, frame 1 No Alert BLASTP hits found Pedant information for DKFZphfbr2_16k22, frame 1 Report for DKFZphfbr2_16k22.1 [LENGTH] 108 [MW] 12281.47 [pi] 8.06 [PROSITE] MYRISTYL 1 [PROSITE] CAMP_PHOΞPHO_SITE 1 [PROSITE] CK2_PHOSPHO_SITE 1 [PROSITE] PKC_PHOSPHO_SITE 1 [PROSITE] ASN_GLYCOSYLATION 1 [KW] Alpha_Beta SEQ MEVSHSTLEPEASFPPPFLSFLVYSKFILSHLFFLNASSPLAFLFLHSLWTGPMLWPLIK PRD ccccccccccccccccccchhhhhhhhhhhhhhhhccccchhhhhhhhccccccchhhhh SEQ AFSKKQSDSSNLHLVMQDVVKNMDΞGGKYTLIPIHSLIYSFSKYLVNI PRD hhhcccccccceeehhhhhhcccccccceeeeeccceeeecccccccc Prosite for DKFZphfbr2 16k22.1 PS00001 36- ->40 ASN GLYCOSYLATION PDOC00001 PS00004 64- ->68 CAMP PHOSPHO SITE PDOC00004 PS00005 63- ->66 PKC PHOSPHO SITE PDOC00005 PS00006 6- ->10 CK2 PHOSPHO SITE PDOC00006 PS00008 86- ->92 MYRISTYL PDOC00008 (No Pfam data available for DKFZphfbr2_16k22.1) DKFZphfbr2_16112 group: transmembrane protein DKFZphfbr2_16112 encodes a novel 267 amino acid protein with similarity to gallus gallus putative transmembrane protein E3-16 The novel protein contains one putative transmembrane domain. In chicken, E3-16 is expressed specifically in the inner ear. No informative BLAST results; no predictive prosite, pfam or SCOP motife The new protein can find application in studying the expression profile of brain-specific genes and as a new marker for neurons involved in perception of hearing. similarity to gallus putative transmembrane protein E3-16 complete cDNA, complete eds, EST hits potental start at Bp 73 matchs kozak consensus PyCCataG TRANSMEMBRANE 1 Sequenced by Qiagen Locus : unknown Insert length: 2042 bp Poly A stretch at pos. 2024, polyadenylation signal at pos. 2003 1 GGGGGCGGCG GAGGCAGAGA CCGAGGCTGC ACCGGCAGAG GCTGCGGGGC 51 GGACGCGCGG GCCGGCGCAG CCATGGTGAA GATTAGCTTC CAGCCCGCCG 101 TGGCTGGCAT CAAGGGCGAC AAGGCTGACA AGGCGTCGGC GTCGGCCCCT 151 GCGCCGGCCT CGGCCACCGA GATCCTGCTG ACGCCGGCTA GGGAGGAGCA 201 GCCCCCACAA CATCGATCCA AGAGGGGGGG CTCAGTGGGC GGCGTGTGCT 251 ACCTGTCGAT GGGCATGGTC GTGCTGCTCA TGGGCCTCGT GTTCGCCTCT 301 GTCTACATCT ACAGATACTT CTTCCTTGCG CAGCTGGCCC GAGATAACTT 351 CTTCCGCTGT GGTGTGCTGT ATGAGGACTC CCTGTCCTCC CAGGTCCGGA 401 CTCAGATGGA GCTGGAAGAG GATGTGAAAA TCTACCTCGA CGAGAACTAC 451 GAGCGCATCA ACGTGCCTGT GCCCCAGTTT GGCGGCGGTG ACCCTGCAGA 501 CATCATCCAT GACTTCCAGC GGGGTCTGAC TGCGTACCAT GATATCTCCC 551 TGGACAAGTG CTATGTCATC GAACTCAACA CCACCATTGT GCTGCCCCCT 601 CGCAACTTCT GGGAGCTCCT CATGAACGTG AAGAGGGGGA CCTACCTGCC 651 GCAGACGTAC ATCATCCAGG AGGAGATGGT GGTCACGGAG CATGTCAGTG 701 ACAAGGAGGC CCTGGGGTCC TTCATCTACC ACCTGTGCAA CGGGAAAGAC 751 ACCTACCGGC TCCGGCGCCG GGCAACGCGG AGGCGGATCA ACAAGCGTGG 801 GGCCAAGAAC TGCAATGCCA TCCGCCACTT CGAGAACACC TTCGTGGTGG 851 AGACGCTCAT CTGCGGGGTG GTGTGAGGCC CTCCTCCCCC AGAACCCCCT 901 GCCGTGTTCC TCTTTTCTTC TTTCCGGCTG CTCTCTGGCC CTCCTCCTTC 951 CCCCTGCTTA GCTTGTACTT TGGACGCGTT TCTATAGAGG TGACATGTCT 1001 CTCCATTCCT CTCCAACCCT GCCCACCTCC CTGTACCAGA GCTGTGATCT 1051 CTCGGTGGGG GGCCCATCTC TGCTGACCTG GGTGTGGCGG AGGGAGAGGC 1101 GATGCTGCAA AGTGTTTTCT GTGTCCCACT GTCTTGAAGC TGGGCCTGCC 1151 AAAGCCTGGG CCCACAGCTG CACCGGCAGC CCAAGGGGAA GGACCGGTTG 1201 GGGGAGCCGG GCATGTGAGG CCCTGGGCAA GGGGATGGGG CTGTGGGGGC 1251 GGGGCGGCAT GGGCTTCAGA AGTATCTGCA CAATTAGAAA AGTCCTCAGA 1301 AGCTTTTTCT TGGAGGGTAC ACTTTCTTCA CTGTCCCTAT TCCTAGACCT 1351 GGGGCTTGAG CTGAGGATGG GACGATGTGC CCAGGGAGGG ACCCACCAGA 1401 GCACAAGAGA AGGTGGCTAC CTGGGGGTGT CCCAGGGACT CTGTCAGTGC 1451 CTTCAGCCCA CCAGCAGGAG CTTGGAGTTT GGGGAGTGGG GATGAGTCCG 1501 TCAAGCACAA CTGTTCTCTG AGTGGAACCA AAGAAGCAAG GAGCTAGGAC 1551 CCCCAGTCCT GCCCCCCAGG AGCACAAGCA GGGTCCCCTC AGTCAAGGCA 1601 GTGGGATGGG CGGCTGAGGA ACGGGGCAGG CAAGGTCACT GCTCAGTCAC 1651 GTCCACGGGG GACGAGCCGT GGGTTCTGCT GAGTAGGTGG AGCTCATTGC 1701 TTTCTCCAAG CTTGGAACTG TTTTGAAAGA TAACACAGAG GGAAAGGGAG 1751 AGCCACCTGG TACTTGTCCA CCCTGCCTCC TCTGTTCTGA AATTCCATCC 1801 CCCTCAGCTT AGGGGAATGC ACCTTTTTCC CTTTCCTTCT CACTTTTGCA 1851 TGTTTTTACT GATCATTCGA TATGCTAACC GTTCTCAGCC CTGAGCCTTG 1901 GAGAGGAGGG CTGTAACGCC TTCAGTCAGT CTCTGGGGAT GAAACTCTTA 1951 AATGCTTTGT ATATTTTCTC AATTAGATCT CTTTTCAGAA GTGTCTATAG 2001 AACAATAAAA ATCTTTTACT TCTGAAAAAA AAAAAAAAAA AA BLAST Results o BLAST result Medlme entries 96325063: Isolation of markers for chondro-osteogenic differentiation using cDNA library subtraction. Molecular cloning and characterization of a gene belonging to a novel multigene family of integral membrane proteins. Peptide information for frame 1 ORF from 73 bp to 873 bp; peptide length: 267 Category: similarity to known protein 1 MVKISFQPAV AGIKGDKADK ASASAPAPAS ATEILLTPAR EEQPPQHRSK 51 RGGSVGGVCY LSMGMVVLLM GLVFASVYIY RYFFLAQLAR DNFFRCGVLY 101 EDSLSSQVRT QMELEEDVKI YLDENYERIN VPVPQFGGGD PADIIHDFQR 151 GLTAYHDISL DKCYVIELNT TIVLPPRNFW ELLMNVKRGT YLPQTYIIQE 201 EMVVTEHVSD KEALGSFIYH LCNGKDTYRL RRRATRRRIN KRGAKNCNAI 251 RHFENTFVVE TLICGVV BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_16112, frame 1 SWISSNEW:ITMB_CHICK INTEGRAL MEMBRANE PROTEIN 2B (TRANSMEMBRANE PROTEIN E3-16)., N = 1, Score = 573, P = 1.4e-55 SWIΞSNEW:ITMB_MOUSE INTEGRAL MEMBRANE PROTEIN 2B (E25B PROTEIN)., N = 1, Score = 559, P = 4.2e-54 SWISSNEW:ITMA_HUMAN INTEGRAL MEMBRANE PROTEIN 2A (E25 PROTEIN)., N = 1, Score = 452, P = 9.1e-43 >SWISSNEW:ITMB_CHICK INTEGRAL MEMBRANE PROTEIN 2B (TRANSMEMBRANE PROTEIN E3-16) . Length = 2 6 2 HSPs: Score = 573 (86.0 bits), Expect = 1.4e-55, P = 1.4e-55 Identities = 118/264 (44%), Positives = 175/264 (66%) Query: 1 MVKISFQPAVAGIKGDKADKASASAPAPASATEILLTPAREEQPPQHRSKRGGΞVGGVCY 60 MVK+SF A+A + A+K ++ ++L+ P + + P+ G C+ Sbjct: 1 MVKVSFNSALA--HKEAANKEEENS QVLILPP-DAKEPEDVVVPAGHKRAWCW 50 Query: 61 -LSMGMVVLLMGLVFASVYIYRYFFLAQLARDNFFRCGVLY-EDSLS SQVRTQM- 112 + G+ +L G++ Y+Y+YF Q + CG+ Y ED LS +Q+++ Sbjct: 51 CMCFGLAFMLAGVILGGAYLYKYFAFQQ GGVYFCGIKYIEDGLSLPESGAQLKSARY 107 Query: 113 -ELEEDVKIYLDENYERINVPVPQFGGGDPADIIHDFQRGLTAYHDISLDKCYVIELNTT 171 +E++++I +E+ E I+VPVP+F DPADI+HDF R LTAY D+SLDKCYVI LNT+ Sbjct: 108 HTIEQNIQILEEEDVEFISVPVPEFADSDPADIVHDFHRRLTAYLDLSLDKCYVIPLNTS 167 Query: 172 IVLPPRNFWELLMNVKRGTYLPQTYIIQEEMVVTEHVSDKEALGSFIYHLCNGKDTYRLR 231 +V+PP+NF ELL+N+K GTYLPQ+Y+I E+M+VT+ + + + LG FIY LC GK+TY+L+ Sbjct: 168 VVMPPKNFLELLINIKAGTYLPQSYLIHEQMIVTDRIENVDQLGFFIYRLCRGKETYKLQ 227 Query: 232 RRATRRRINKRGAKNCNAIRHFENTFVVETLIC 264 R+ + I KR A NC IRHFEN F +ETLIC Sbjct: 228 RKEAMKGIQKREAVNCRKIRHFENRFAMETLIC 260 Pedant information for DKFZphfbr2_16112, frame 1 Report for DKFZphfbr2_16112.1 [LENGTH] 267 [MW] 30223.94 [pi] 8.16 [HOMOL] SWISSNEW:ITMB_CHICK INTEGRAL MEMBRANE PROTEIN 2B (TRANSMEMBRANE PROTEIN E3-16) le-49 [PROSITE] PRENYLATION 1 [PROSITE] MYRISTYL 5 [PROSITE] CAMP_PHOSPHO_SITE 2 [PROSITE] CK2_PHOSPHO_SITE 3 [PROSITE] TYR_PHOSPHO_SITE 1 [PROSITE] PKC_PHOSPHO_SITE 4 [PROSITE] ASN_GLYCOSYLATION 1 [KW] TRANSMEMBRANE 1 [KW] LOW COMPLEXITY 15.36 % SEQ MVKISFQPAVAGIKGDKADKASASAPAPASATEILLTPAREEQPPQHRSKRGGSVGGVCY SEG xxxxxxxxxxxxxxxx PRD ccccccccchhhhhhhhhhhhhhhhhccccccceeecccccccccccccccccccccchh MEM MMMMMMMMM SEQ LSMGMVVLLMGLVFAΞVYIYRYFFLAQLARDNFFRCGVLYEDSLSSQVRTQMELEEDVKI SEG .. xxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhcchhhhhhhhhhccceeeeeecccccccchhhhhhhhhhhhh MEM MMMMMMMMMMMMMMMMMMM SEQ YLDENYERINVPVPQFGGGDPADIIHDFQRGLTAYHDISLDKCYVIELNTTIVLPPRNFW SEG PRD hhcccceeeeccccccccccccchhhhhhhhhhhhhhhcccceeeeeccceeecccchhh MEM SEQ ELLMNVKRGTYLPQTYIIQEEMVVTEHVSDKEALGSFIYHLCNGKDTYRLRRRATRRRIN SEG xxxxxxxxxxxx PRD hhhhhhcccccccceeeeehhhhhhhccccchhhhhheeeccccchhhhhhhhhhhhhhh MEM SEQ KRGAKNCNAIRHFENTFVVETLICGVV SEG xx PRD hhhhccceeeecccchhhhhheeeccc MEM Prosite for DKFZphfbr2_16112.1 PS00001 169->173 ASN_GLYCOSYLATION PDOC00001 PS00004 187->191 CAMP_PHOSPHO_SITE PDOC00004 PS00004 232->236 CAMP_PHOSPHO_SITE PDOC00004 PS00005 49->52 PKC_PHOSPHO_SITE PDOC00005 PS00005 209->212 PKC_PHOSPHO_SITE PDOC00005 PS00005 227->230 PKC_PHOSPHO_SITE PDOC00005 PS00005 235->238 PKC_PHOSPHO_SITE PDOC00005 PS00006 30->34 CK2_PHOSPHO_SITE PDOC00006 PS00006 110->114 CK2_PHOSPHO_SITE PDOC00006 PS00006 209->213 CK2_PHOSPHO_SITE PDOC00006 PS00007 119->127 TYR_PHOSPHO_SI E PDOC00007 PΞ00008 52->58 MYRISTYL PDOC00008 PS00008 53->59 MYRISTYL PDOC00008 PS00008 71->77 MYRISTYL PDOC00008 PS00008 138->144 MYRISTYL PDOC00008 PS00008 243->249 MYRISTYL PDOC00008 PS00294 264->268 PRENYLATION PDOC00266 (No Pfam data available for DKFZphfbr2_16112.1) DKFZphfbr2_22f21 group: brain derived DKFZphfbr2_22f21 encodes a novel 567 amino acid protein with weak similarity to C. elegans cosmide C18C4.5 No informative BLAST results; no predictive prosite, pfam or SCOP motife The new protein can find application in studying the expression profile of brain-specific genes . weak similarity to C. elegans C18C4.5 EST HSAA6531/HSAA5273/ defines splice variant, or unspliced cDNA additional -180 Bp at position 250 Sequenced by AGOWA Locus: /map="311.4 cR from top of Chrl4 linkage group" Insert length: 1910 bp Poly A stretch at pos. 1887, polyadenylation signal at pos. 1867 1 TGGGCCCTTA GCAACGGCCT GGCGACGGTT TCCTTGCTGC TGCAGCCCCC 51 GTCGGCTCCT CTTTTCCAGT CCTCCACTGC CGGGGCTGGG CCCGGCCGCG 101 GGAAGGACCG AAGGGGATAC AGCGTGTCCC TGCGGCGGCT GCAAGAGGAC 151 TAAGCATGGA TGGCAGCCGG AGAGTCAGAG CAACCTCTGT CCTTCCCAGA 201 TATGGTCCAC CGTGCCTATT TAAAGGACAC TTGAGCACCA AAAGTAATGC 251 TGCAGTAGAC TGCTCGGTTC CAGTAAGCAT GAGTACCAGC ATAAAGTATG 301 CAGACCAACA ACGAAGAGAG AAACTCAAAA AGGAATTAGC ACAATGTGAA 351 AAAGAGTTCA AATTAACTAA AACTGCAATG CGAGCCAATT ATAAAAATAA 401 TTCCAAGTCA CTTTTTAATA CCTTACAAGA GCCCTCAGGC GAACCGCAAA 451 TTGAGGATGA CATGTTAAAA GAAGAAATGA ATGGATTTTC ATCCTTTGCA 501 AGGTCACTAG TACCCTCTTC AGAGAGACTA CACCTAAGTC TACATAAATC 551 CAGTAAAGTC ATCACAAATG GTCCTGAGAA GAACTCCAGT TCCTCCCCGT 601 CCAGTGTGGA TTATGCAGCC TCCGGGCCCC GGAAACTGAG CTCTGGAGCC 651 CTGTATGGCA GAAGGCCCAG AAGCACATTC CCAAATTCCC ACCGGTTTCA 701 GTTAGTCATT TCGAAAGCAC CCAGTGGGGA TCTTTTGGAT AAACATTCTG 751 AACTCTTTTC TAACAAACAA TTGCCATTCA CTCCTCGCAC TTTAAAAACA 801 GAAGCAAAAT CTTTCCTGTC ACAGTATCGC TATTATACAC CTGCCAAAAG 851 AAAAAAGGAT TTTACAGATC AACGGATAGA AGCTGAAACC CAGACTGAAT 901 TAAGCTTTAA ATCTGAGTTG GGGACAGCTG AGACTAAAAA CATGACAGAT 951 TCAGAAATGA ACATAAAGCA GGCATCTAAT TGTGTGACAT ATGATGCCAA 1001 AGAAAAAATA GCTCCTTTAC CTTTAGAAGG GCATGACTCA ACATGGGATG 1051 AGATTAAGGA TGATGCTCTT CAGCATTCCT CACCAAGGGC AATGTGTCAG 1101 TATTCCCTGA AGCCCCCTTC AACTCGTAAA ATCTACTCTG ATGAAGAAGA 1151 ACTGTTGTAT CTGAGTTTCA TTGAAGATGT AACAGATGAA ATTTTGAAAC 1201 TTGGTTTATT TTCAAACAGG TTTTTAGAAC GACTGTTCGA GCGACATATA 1251 AAACAAAATA AACATTTGGA GGGGGAAAAA ATGCGCCACC TGCTGCATGT 1301 CCTGAAAGTA GACTTAGGCT GCACATCGGA GGAAAACTCG GTAAAGCAAA 1351 ATGATGTTGA TATGTTGAAT GTATTTGATT TTGAAAAGGC TGGGAATTCA 1401 GAACCAAATA AATTAAAAAA TGAAAGTGAA GTAACAATTC AGCAGGAACG 1451 TCAACAATAC CAAAAGGCTT TGGATATGTT ATTGTCGGCA CCAAAGGATG 1501 AGAACGAGAT ATTCCCTTCA CCAACTGAAT TTTTCATGCC TATTTATAAA 1551 TCAAAGCATT CAGAAGGGGT TATAATTCAA CAGGTGAATG ATGAAACAAA 1601 TCTTGAAACT TCAACTTTGG ATGAAAATCA TCCAAGTATT TCAGACAGTT 1651 TAACAGATCG GGAAACTTCT GTGAATGTCA TTGAAGGTGA TAGTGACCCT 1701 GAAAAGGTTG AGATTTCAAA TGGATTATGT GGTCTTAACA CATCACCCTC 1751 CCAATCTGTT CAGTTCTCCA GTGTCAAAGG CGACAATAAT CATGACATGG 1801 AGTTATCAAC TCTTAAAATC ATGGAAATGA GCATTGAGGA CTGCCCTTTG 1851 GATGTTTAAT CTTCATTAAT AAATACCTCA AATGGCCAGT AAAAAAAAAA 1901 AAAAAAAAAA BLAST Results Entry HS477360 from database EMBL: human STS WI-14643. Length = 418 Minus Strand HSPs: Score = 1850 (277.6 bits), Expect = 2.5e-77, P = 2.5e-77 Identities = 392/405 (96%), Positives = 392/405 (96%), Strand = Minus / Plus Medlme entries No Medlme entry Peptide information for frame 3 ORF from 156 bp to 1856 bp; peptide length: 567 Category: similarity to unknown protein 1 MDGSRRVRAT SVLPRYGPPC LFKGHLSTKΞ NAAVDCSVPV SMSTSIKYAD 51 QQRREKLKKE LAQCEKEFKL TKTAMRANYK NNSKSLFNTL QEPSGEPQIE 101 DDMLKEEMNG FSSFARSLVP SSERLHLSLH KSSKVITNGP EKNSSSSPSS 151 VDYAASGPRK LSSGALYGRR PRSTFPNSHR FQLVISKAPS GDLLDKHSEL 201 FSNKQLPFTP RTLKTEAKSF LSQYRYYTPA KRKKDFTDQR IEAETQTELS 251 FKSELGTAET KNMTDSEMNI KQASNCVTYD AKEKIAPLPL EGHDSTWDEI 301 KDDALQHSSP RAMCQYSLKP PSTRKIYSDE EELLYLSFIE DVTDEILKLG 351 LFSNRFLERL FERHIKQNKH LEGEKMRHLL HVLKVDLGCT SEENSVKQND 401 VDMLNVFDFE KAGNSEPNKL KNESEVTIQQ ERQQYQKALD MLLΞAPKDEN 451 EIFPSPTEFF MPIYKSKHSE GVIIQQVNDE TNLETSTLDE NHPSISDSLT 501 DRETSVNVIE GDSDPEKVEI SNGLCGLNTS PSQSVQFSSV KGDNNHDMEL 551 STLKIMEMSI EDCPLDV BLASTP hits Entry CEC18C4_3 from database TREMBL: "C18C4.5"; Caenorhabditis elegans cosmid C18C4. Length = 1091 Score = 98 (34.5 bits), Expect = 0.29, P = 0.25 Identities = 105/470 (22%), Positives = 192/470 (40%) Alert BLASTP hits for DKFZphfbr2_22f21, frame 3 No Alert BLASTP hits found Pedant information for DKFZphfbr2_22f21, frame 3 Report for DKFZphfbr2_22 21.3 [LENGTH] 567 [MW] 64120.02 [pi] 5.68 [PROSITE] AMIDATION 1 [PROSITE] MYRISTYL 3 [PROSITE] CAMP_PHOSPHO_SITE 1 [PROSITE] CK2_PHOSPHO_SITE 16 [PROSITE] PKC_PHOSPHO_SITE 18 [PROSITE] ASN_GLYCOSYLATION 4 [KW] All_Alpha [KW] LOW COMPLEXITY 1.23 % SEQ MDGSRRVRATSVLPRYGPPCLFKGHLSTKSNAAVDCSVPVSMSTSIKYADQQRREKLKKE SEG PRD cccccceeeeeeccccccccccccccccccceeeecccccccchhhhhhhhhhhhhhhhh SEQ LAQCEKEFKLTKTAMRANYKNNSKSLFNTLQEPSGEPQIEDDMLKEEMNGFSSFARSLVP SEG PRD hhhhhhhhhhhhhhhhhhhccccccceeecccccccchhhhhhhhhhhccccccceeecc SEQ SSERLHLSLHKSSKVITNGPEKNSSSSPΞSVDYAASGPRKLSSGALYGRRPRSTFPNSHR SEG xxxxxxx PRD ccchhhhhhhhceeeecccccccccccccccccccccccccccccccccccccccccccc SEQ FQLVISKAPSGDLLDKHSELFSNKQLPFTPRTLKTEAKSFLSQYRYYTPAKRKKDFTDQR SEG PRD cceeeeeccccccccccccccccccccccccchhhhhhhhhhhhhccccccchhhhhhhh SEQ IEAETQTELSFKSELGTAETKNMTDSEMNIKQASNCVTYDAKEKIAPLPLEGHDSTWDEI SEG PRD hhhhhhhhhhhhhhccccccccccchhhhhhhccceeehhhhhhcccccccccccccccc SEQ KDDALQHSSPRAMCQYSLKPPSTRKIYSDEEELLYLSFIEDVTDEILKLGLFSNRFLERL SEG PRD cccccccccccccccccccccccccccchhhhhhhhhhhhhhhhhhhhhhccchhhhhhh SEQ FERHIKQNKHLEGEKMRHLLHVLKVDLGCTSEENSVKQNDVDMLNVFDFEKAGNSEPNKL SEG PRD hhhhhhhhhhcccchhhhhhhhhccccccccccccccccccccceeeecccccccccccc SEQ KNESEVTIQQERQQYQKALDMLLSAPKDENEIFPSPTEFFMPIYKSKHSEGVIIQQVNDE SEG PRD hhhhhhhhhhhhhhhhhhhhhhhcccccccccccccccccccccccccccceeeeecccc SEQ TNLETSTLDENHPSISDSLTDRETSVNVIEGDSDPEKVEISNGLCGLNTSPSQSVQFSSV SEG PRD ccccccccccccccccccccccccceeecccccccceeeeccccccccccccceeeeecc SEQ KGDNNHDMELSTLKIMEMSIEDCPLDV SEG PRD ccccccchhhhhhhhhhhhhccccccc Prosite for DKFZphfbr2_22f21.3 PS00001 81->85 ASN_GLYCOSYLATION PDOC00001 PS00001 143->147 ASN_GLYCOSYLATION PDOC00001 PS00001 262->266 ASN_GLYCOSYLATION PDOC00001 PS00001 422->426 ASN_GLYCOSYLATION PDOC00001 PS00004 159->163 CAMP_PHOSPHO_SITE PDOC00004 PS00005 4->7 PKC_PHOSPHO_SITE PDOC00005 PS00005 27->30 PKC_PHOSPHO_SITE PDOC00005 PS00005 45->48 PKC_PHOSPHO_ΞITE PDOC00005 PS00005 122->125 PKC_PHOSPHO_SITE PDOC00005 PS00005 132->135 PKC_PHOSPHO_SITE PDOC00005 PS00005 178->181 PKC_PHOSPHO_SITE PDOC00005 PS00005 202->205 PKC_PHOΞPHO_SITE PDOC00005 PS00005 209->212 PKC_PHOSPHO_SITE PDOC00005 PS00005 212->215 PKC_PHOSPHO_SITE PDOC00005 PS00005 250->253 PKC_PHOSPHO_SITE PDOC00005 PS00005 309->312 PKC_PHOSPHO_SITE PDOC00005 PS00005 317->320 PKC_PHOSPHO_SITE PDOC00005 PS00005 322->325 PKC_PHOSPHO_SI E PDOC00005 PS00005 353->356 PKC_PHOSPHO_SITE PDOC00005 PS00005 395->398 PKC_PHOSPHO_SITE PDOC00005 PS00005 500->503 PKC_PHOSPHO_SITE PDOC00005 PS00005 539->542 PKC_PHOSPHO_SITE PDOC00005 PS00005 552->555 PKC_PHOSPHO_SITE PDOC00005 PS00006 89->93 CK2_PHOSPHO_SITE PDOC00006 PS00006 149->153 CK2_PHOSPHO_SITE PDOC00006 PS00006 245->249 CK2_PHOSPHO_SITE PDOC00006 PS00006 264->268 CK2_PHOSPHO_SITE PDOC00006 PS00006 295->299 CK2_PHOSPHO_SITE PDOC00006 PS00006 328->332 CK2_PHOSPHO_SITE PDOC00006 PS00006 337->341 CK2_PHOSPHO_ΞITE PDOC00006 PΞ00006 390->394 CK2_PHOSPHO_SITE PDOC00006 PS00006 455->459 CK2_PHOSPHO_SITE PDOC00006 PS00006 481->485 CK2_PHOSPHO_SITE PDOC00006 PS00006 486->490 CK2_PHOSPHO_SITE PDOC00006 PΞ00006 494->498 CK2_PHOSPHO_SITE PDOC00006 PS00006 498->502 CK2_PHOSPHO_SITE PDOC00006 PS00006 500->504 CK2_PHOSPHO_SITE PDOC00006 PS00006 513->517 CK2_PHOSPHO_SITE PDOC00006 PS00006 559->563 CK2_PHOSPHO_SITE PDOC00006 PS00008 164->170 MYRISTYL PDOC00008 PS00008 256-> 2 62 MYRISTYL PDOC00008 PS00008 350->356 MYRISTYL PDOC00008 PS00009 167->171 AMIDATION PDOC00009 (No Pfam data available for DKFZphfbr2_22f21.3) DKFZphfbr2_22hl3 group: transmembrane protein DKFZphfbr2_22hl3 encodes a novel 520 ammo acid protein, with similarity to Drosophila melanogaster EG:39E1.3. The protein contains an ATP/GTP A Prosite pattern (P-loop) . This loop interacts with one of the phosphate groups of a A or G nucleotide. It is found m numerous ATP- or GTP-binding proteins, such as ATP synthase alpha and beta subunits, Myosin heavy chains, Kinesin heavy chains and kinesin-like proteins, Dynamins and dynamin-like proteins, several kinases, DNA and RNA helicases, GTP-bind g elongation factors and the Ras family of GTP-bmding proteins. Additionally, the novel protein contains one putative transmembran domain. No informative BLAST results; no predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of brain-specific genes and as a new marker for neuronal cells. AC004780_1, differences to predicted genmodel membrane regions: 1 AC004780_1, differences to predicted genmodel complete cDNA, complete eds, EST hits on genomic level encoded by AC004780, differences to predicted genmodel 1 TRANSMEMBRANE 1 Sequenced by AGOWA Locus : unknown Insert length: 2292 bp Poly A stretch at pos. 2272, polyadenylation signal at pos. 2255 1 GGGGGAGGGA ACTGATCTCA GCTCGGGCCC GCGTTACATC CTCCTCCTCT 51 TCTTCCTTCG GCCCAGCTTT CCTTAGGGGC TGCAACCCGG ACGCCGAGGC 101 CGGTTTCGGA GTGGGGAGTG CCCATTTTCT CTCCTTCCCA CGTTCCTGGC 151 CCCCAGACGC CATTTGCAGG CGGGTGGCTT GGGTCAGCCT CCCCGCCCCC 201 ACCCGACTCC CGTCACGGGA GAGCGCACAC CGCGCCCCGA GAACCAATCA 251 GCAGCCGCGT TAGGTAACCA TGTCTGAGTC TGGACACAGT CAGCCTGGAC 301 TCTATGGGAT AGAGCGGCGG CGACGGTGGA AGGAGCCTGG CTCTGGTGGC 351 CCCCAGAATC TCTCTGGGCC TGGTGGTCGG GAGAGGGACT ACATTGCACC 401 ATGGGAAAGA GAGAGAAGGG ATGCCAGCGA AGAGACAAGC ACTTCCGTCA 451 TGCAGAAAAC CCCCATCATC CTCTCAAAAC CTCCAGCAGA GCGGTCAAAA 501 CAGCCACCAC CTCCAACAGC CCCTGCTGCC CCGCCTGCTC CAGCCCCTCT 551 GGAGAAGCCC ATCGTTCTCA TGAAGCCACG GGAGGAGGGG AAGGGGCCTG 601 TGGCCGTGAC AGGTGCCTCT ACCCCTGAGG GCACCGCCCC ACCACCCCCT 651 GCAGCCCCTG CGCCACCCAA GGGGGAGAAG GAGGGGCAGA GACCCACACA 701 GCCTGTGTAC CAGATCCAGA ACCGGGGCAT GGGCACTGCC GCACCAGCAG 751 CCATGGACCC TGTCGTGGGT CAGGCCAAAC TACTGCCCCC AGAGCGCATG 801 AAGCACAGCA TCAAGTTGGT GGATGACCAG ATGAATTGGT GTGACAGTGC 851 CATCGAGTAC CTGTTGGATC AGACTGATGT GTTGGTGGTT GGTGTCCTGG 901 GCCTCCAGGG GACAGGCAAG TCCATGGTCA TGTCATTGTT GTCAGCCAAC 951 ACTCCAGAGG AGGACCAGAG GACTTATGTT TTCCGGGCCC AGAGCGCTGA 1001 AATGAAGGAA CGAGGGGGCA ACCAGACCAG TGGCATCGAC TTCTTTATTA 1051 CCCAAGAACG GATTGTTTTC CTGGACACAC AGCCCATCCT GAGCCCTTCT 1101 ATCCTAGACC ATCTCATCAA TAATGACCGC AAACTGCCTC CAGAGTACAA 1151 CCTTCCCCAC ACTTACGTTG AAATGCAGTC ACTCCAGATT GCTGCCTTCC 1201 TTTTCACGGT CTGCCATGTG GTGATTGTTG TCCAGGACTG GTTCACAGAC 1251 CTCAGTCTCT ACAGGTTCCT GCAGACAGCA GAGATGGTGA AGCCCTCCAC 1301 CCCATCCCCC AGCCACGAGT CCAGCAGCTC ATCGGGCTCC GATGAAGGCA 1351 CCGAGTACTA CCCCCACCTA GTCTTCTTGC AGAACAAAGC TCGCCGAGAG 1401 GACTTCTGTC CTCGGAAGCT GCGGCAGATG CACCTGATGA TTGACCAGCT 1451 CATGGCCCAC TCCCACCTGC GTTACAAGGG AACTCTGTCC ATGTTACAAT 1501 GCAATGTCTT CCCGGGGCTT CCACCTGACT TCCTGGACTC TGAGGTCAAC 1551 TTATTCCTGG TACCCTTCAT GGACAGTGAA GCAGAGAGTG AAAACCCACC 1601 AAGAGCAGGA CCTGGTTCCA GCCCACTCTT CTCCCTGCTG CCTGGGTATC 1651 GTGGCCACCC CAGTTTCCAG TCCTTGGTGA GCAAGCTCCG GAGCCAAGTG 1701 ATGTCCATGG CCCGGCCACA GCTGTCACAC ACGATCCTCA CCGAGAAGAA 1751 CTGGTTCCAC TACGCTGCCC GGATCTGGGA TGGGGTGAGA AAGTCCTCTG 1801 CTCTGGCAGA GTACAGCCGC CTGCTGGCCT GAGGCCAAGG AGAGGAATGT 1851 CATGCAGGGG ACCTCCTGGG TCCGCAGTGT ACTGCGAGGG AGCACAGATG 1901 TCCATCCCCC GCTGGGGTGG AGAGCGGCAG CAGGCCTGAT GGATGAGGGA 1951 TCGTGGCTTC CCGGCCCAGA GACATGAGGT GTCCAGGGCC AGGCCCCCCA 2001 CCCTCAGTTG GGGCTGTTCC GGGGGTGACT GTGAGCGATC CCACCCCAAA 2051 CCTGAGATGG GGTAGCCCGT CCTGTGTCCT CCACAGGGAC AAGCAGTGGG 2101 AGGAGTCTGA ATGGTCACCA GGAAGCCCGG GCTCCATCTT GACCTCCTTT 2151 TTCAGGGACA GGAGCAACAG GCCCCTCTTC CCTGACTCTA AGCCCTTCCC 2201 TGTAAGGTGA GGCAGGGTCT GGAGAGCTCT TTATTGGAAC AGATCTGGTG 2251 GTTCAAATAA ACACAGTCAT GCAAAAAAAA AAAAAAAAAA AA BLAST Results Entry AC004780 from database EMBL: Homo sapiens chromosome 19, cosmid F17127, complete sequence. Score = 2616, P = O.Oe+00, identities = 524/525 15 exons Bp 8031-31789 Medlme entries No Medlme entry Peptide information for frame 3 ORF from 270 bp to 1829 bp; peptide length: 520 Category: similarity to unknown protein Prosite motifs: ATP GTP A (211-219) 1 MΞESGHSQPG LYGIERRRRW KEPGSGGPQN LSGPGGRERD YIAPWERERR 51 DASEETSTSV MQKTPIILSK PPAERSKQPP PPTAPAAPPA PAPLEKPIVL 101 MKPREEGKGP VAVTGASTPE GTAPPPPAAP APPKGEKEGQ RPTQPVYQIQ 151 NRGMGTAAPA AMDPVVGQAK LLPPERMKHS IKLVDDQMNW CDSAIEYLLD 201 QTDVLVVGVL GLQGTGKSMV MSLLSANTPE EDQRTYVFRA QSAEMKERGG 251 NQTSGIDFFI TQERIVFLDT QPILSPSILD HLINNDRKLP PEYNLPHTYV 301 EMQSLQIAAF LFTVCHVVIV VQDWFTDLSL YRFLQTAEMV KPSTPSPSHE 351 SSSSSGSDEG TEYYPHLVFL QNKARREDFC PRKLRQMHLM IDQLMAHSHL 401 RYKGTLSMLQ CNVFPGLPPD FLDSEVNLFL VPFMDSEAES ENPPRAGPGS 451 SPLFSLLPGY RGHPSFQSLV SKLRSQVMSM ARPQLSHTIL TEKNWFHYAA 501 RIWDGVRKSS ALAEYSRLLA BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_22hl3, frame 3 TREMBL:AC004780_1 product: "F17127_l"; Homo sapiens chromosome 19, cosmid F17127, complete sequence., N = 2, Score = 1264, P = 1.3e-231 TREMB :CEY54E2A_1 gene: "Y54E2A.2"; Caenorhabditis elegans cosmid Y54E2A, N = 2, Score = 219, P = 1.4e-15 >TREMBL:AC004780_1 product: "F17127_l"; Homo sapiens chromosome 19, cosmid F17127, complete sequence. Length = 528 HSPs: Score = 1264 (189.6 bits), Expect = 1.3e-231, Sum P(2) = 1.3e-231 Identities = 254/302 (84%), Positives = 264/302 (87%) Query: 46 ERERRDASEETSTSVMQKTPIILSKPPAERSKQPPPPTAPAAPPAPAPLEKPIVLMKPRE 105 E+ER D+ + S +Q+T + R + P + A APLEKPIVLMKPRE Sbjct: 39 EKER-DSDSDFΞP—LQQTEGCQRRDKHFRHAENPHHPLKTSSRA-APLEKPIVLMKPRE 94 Query: 106 EGKGPVAVTGASTPEGTAPPPPAAPAPPKGEKEGQRPTQPVYQIQNRGMGTAAPAAMDPV 165 EGKGPVAVTGASTPEGTAPPPPAAPAPPKGEKEGQRPTQPVYQIQNRGMGTAAPAAMDPV Sbjct: 95 EGKGPVAVTGASTPEGTAPPPPAAPAPPKGEKEGQRPTQPVYQIQNRGMGTAAPAAMDPV 154 Query: 166 VGQAKLLPPERMKHSIKLVDDQMNWCDSAIEYLLDQTDVLVVGVLGLQGTGKSMVMSLLS 225 VGQAKLLPPERMKHSIKLVDDQMNWCDSAIEYLLDQTDVLVVGVLGLQGTGKSMVMSLLS Sbjct: 155 VGQAKLLPPERMKHSIKLVDDQMNWCDSAIEYLLDQTDVLVVGVLGLQGTGKSMVMSLLS 214 Query: 226 ANTPEEDQRTYVFRAQSAEMKERGGNQTSGIDFFITQERIVFLDTQPILΞPSILDHLINN 285 ANTPEEDQRTYVFRAQSAEMKERGGNQTSGIDFFITQERIVFLDTQPILSPSILDHLINN Sbjct: 215 ANTPEEDQRTYVFRAQSAEMKERGGNQTSGIDFFITQERIVFLDTQPILSPSILDHLINN 274 Query: 286 DRKLPPEYNLPHTYVEMQSLQIAAFLFTVCHVVIVVQDWFTDLSLYRFLQTAEMVKPSTP 345 DRKLPPEYNLPHTYVEMQSLQIAAFLFTVCHVVIVVQDWFTDLSLYR K ++ Sbjct: 275 DRKLPPEYNLPHTYVEMQSLQIAAFLFTVCHVVIVVQDWFTDLSLYRLWDLGCKCKΞNSH 334 Query: 346 SP 347 SP Sbjct: 335 SP 336 Score = 993 (149.0 bits), Expect = 1.3e-231, Sum P(2) = 1.3e-231 Identities == 189/189 (100%), Positives = 189/189 (100%) Query: 332 RFLQTAEMVKPSTPSPSHESSSSSGSDEGTEYYPHLVFLQNKARREDFCPRKLRQMHLMI 391 RFLQTAEMVKPSTPSPSHESSSSSGSDEGTEYYPHLVFLQNKARREDFCPRKLRQMHLMI Sbjct: 340 RFLQTAEMVKPΞTPSPSHESSSSSGSDEGTEYYPHLVFLQNKARREDFCPRKLRQMHLMI 399 Query: 392 DQLMAHSHLRYKGTLSMLQCNVFPGLPPDFLDSEVNLFLVPFMDSEAESENPPRAGPGSS 451 DQLMAHSHLRYKGTLSMLQCNVFPGLPPDFLDΞEVNLFLVPFMDSEAESENPPRAGPGSS Sbjct: 400 DQLMAHSHLRYKGTLSMLQCNVFPGLPPDFLDSEVNLFLVPFMDSEAESENPPRAGPGSS 459 Query: 452 PLFSLLPGYRGHPSFQSLVSKLRSQVMSMARPQLSHTILTEKNWFHYAARIWDGVRKSΞA 511 PLFSLLPGYRGHPSFQSLVSKLRSQVMSMARPQLSHTILTEKNWFHYAARIWDGVRKSSA Sbjct: 460 PLFSLLPGYRGHPSFQSLVSKLRSQVMSMARPQLSHTILTEKNWFHYAARIWDGVRKSSA 519 Query: 512 LAEYSRLLA 520 LAEYSRLLA Sbjct: 520 LAEYSRLLA 528 Pedant information for DKFZphfbr2_22hl3, frame 3 Report for DKFZphfbr2_22hl3.3 [LENGTH] 520 [MW] 57650.81 [pi] 6.52 [HOMOL] TREMBL:AC004780_1 product: "F17127_l"; Homo sapiens chromosome 19, cosmid F17127, complete sequence. 0.0 [PROSITE] A P_GTP_A 1 [PROSITE] MYRISTYL 8 [PROSITE] CAMP_PHOSPHO_SITE [PROSITE] CK2_PHOSPHO_SITE [PROSITE] GLYCOSAMINOGLYCAN 1 [PROSITE] PKC_PHOSPHO_SITE 3 [PROSITE] ASN_GLYCOSYLATION 2 [KW] TRANSMEMBRANE 1 [KW] LOW COMPLEXITY 11.73 % SEQ MSESGHSQPGLYGIERRRRWKEPGSGGPQNLSGPGGRERDYIAPWERERRDASEETSTSV SEG PRD cccccccccccccccccccccccccccccccccccccceeeeehhhhhhhhhccccccee MEM SEQ MQKTPIILSKPPAERSKQPPPPTAPAAPPAPAPLEKPIVLMKPREEGKGPVAVTGASTPE SEG xxxxxxxxxxxxxxx PRD eeccceeecccccccccccccccccccccccccccceeeeeccccccccceeeecccccc MEM SEQ GTAPPPPAAPAPPKGEKEGQRPTQPVYQIQNRGMGTAAPAAMDPVVGQAKLLPPERMKHS SEG .. xxxxxxxxxxx PRD cccccccccccccccccccccccceeeeeeccccccccccccceeecceeecccchhhhh MEM SEQ IKLVDDQMNWCDSAIEYLLDQTDVLVVGVLGLQGTGKSMVMSLLSANTPEEDQRTYVFRA SEG xxxxxxxxxxxxxxxxxxx PRD hhhhcccchhhhhhhhhhccccceeeeeecccccccchhhhhhhhccccchhhhhheeee MEM SEQ QSAEMKERGGNQTSGIDFFITQERIVFLDTQPILSPSILDHLINNDRKLPPEYNLPHTYV SEG PRD hhhhhhhcccccceeeeeeeecceeeeeeccccccccccccccccccccccccccccchh MEM SEQ EMQSLQIAAFLFTVCHVVIVVQDWFTDLSLYRFLQTAEMVKPSTPSPSHESSSSSGSDEG SEG xxxxxxxxxxxxxxxx ... PRD hhhhhhhhhhhhhhhheeeeeeeccchhhhhhhhhhhhhhhccccccccccccccccccc MEM MMMMMMMMMMMMMMMMMMMMMMM SEQ TEYYPHLVFLQNKARREDFCPRKLRQMHLMIDQLMAHSHLRYKGTLSMLQCNVFPGLPPD SEG PRD cccccceeeehhhhhhhcccccchhhhhhhhhhhhhhhhhhccccccccccccccccccc MEM SEQ FLDSEVNLFLVPFMDSEAESENPPRAGPGΞSPLFSLLPGYRGHPSFQSLVSKLRSQVMSM SEG PRD chhhhhheeeeeccccccccccccccccccccceeeccccccccchhhhhhhhhhhhhhh MEM SEQ ARPQLSHTILTEKNWFHYAARIWDGVRKSSALAEYSRLLA SEG PRD hhhhhhhheeeccchhhhhhhhhhhhcchhhhhhhhhccc MEM Prosite for DKFZphfbr2_22hl3.3 PS00001 30->34 ASN_GLYCOSYLATION PDOC00001 PS00001 251->255 ASN_GLYCOΞYLATION PDOC00001 PS00002 32->36 GLYCOSAMINOGLYCAN PDOC00002 PS00004 507->511 CAMP_PHOSPHO_SITE PDOC00004 PS00005 180->183 PKC_PHOSPHO_SITE PDOC00005 PS00005 215->218 PKC_PHOSPHO_SITE PDOC00005 PS00005 491->494 PKC_PHOSPHO_SITE PDOC00005 PS00006 117->121 CK2_PHOΞPHO_SITE PDOC00006 PS00006 193->197 CK2_PHOSPHO_SITE PDOC00006 PS00006 22 8->232 CK2_PHOSPHO_ΞITE PDOC00006 PS00006 254->258 CK2_PHOSPHO_SITE PDOC00006 PS00006 277->281 CK2_PHOSPHO_SITE PDOC00006 PS00006 298->302 CK2_PHOSPHO_SITE PDOC00006 PS00006 355->359 CK2_PHOSPHO_SITE PDOC00006 PS00006 436->440 CK2_PHOSPHO_SITE PDOC00006 PS00008 26->32 MYRISTYL PDOC00008 PS00008 139->145 MYRISTYL PDOC00008 PΞ00008 153->159 MYRISTYL PDOC00008 PS00008 211->217 MYRISTYL PDOC00008 PS00008 214->220 MYRISTYL PDOC00008 PS00008 249->255 MYRISTYL PDOC00008 PS00008 356->362 MYRISTYL PDOC00008 PS00008 505->511 MYRISTYL PDOC00008 PS000Ϊ7 211->219 ATP GTP A PDOC00017 (No Pfam data available for DKFZphfbr2 22hl3.3) DKFZphfbr2_22ι4 group: brain derived DKFZphfbr2_22ι4.1 encodes a novel 228 amino acid protein with similarity to the N-termmus of human p52rIPK. No informative BLAST results; no predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of brain-specific genes . similarity to Human P52rIPK N-terminus complete cDNA, complete eds, few EST hits function of P52rIPK, repressor of p58IPK protein kinase inhibitor upstream regulator of interferon induced proteins Sequenced by AGOWA Locus: unknown Insert length: 4748 bp Poly A stretch at pos. 4726, polyadenylation signal at pos. 4709 1 TGGGTCCGGT CCTAGGGTCA CACCCACCGC AGGGTCTGGC TTGGTACAGT 51 TGGGTGCATG CAGAAGTAGG TGGAGCTGCT GTTGCAGCCT TGAGAGAGTT 101 TTATTGTAAA ACTCTTGTAA TTTATAGTAA TCGGAGGGGA AAACACCTCT 151 TCCTTTTAAT TGCTCTGAGG ACCGCTGCCA AAGAAACGCA GTAGATCCGC 201 TCCCTCTTGG GGGCGGGGAG AAAGAACGGG TTGTGTCCGC CATGTTGGTG 251 AAGTCAAGCG AAGGCGACTA GAGCTCCAGG AGGGCCAGTT CTGTGGGCTC 301 TAGTCGGCCA TATTAATAAA GAGAAAGGGA AGGCTGACCG TCCTTCGCCT 351 CCGCCCCCAC ATACACACCC CTTCTTCCCA CTCCGCTCTC ACGACTAAGC 401 TCTCACGATT AAGGCACGCC TGCCTCGATT GTCCAGCCTC TGCCAGAAGA 451 AAGCTTAGCA GCCAGCGCCT CAGTAGAGAC CTAAGGGCGC TGAATGAGTG 501 GGAAAGGGAA ATGCCGACCA ATTGCGCTGC GGCGGGCTGT GCCACTACCT 551 ACAACAAGCA CATTAACATC AGCTTCCACA GGTTTCCTTT GGATCCTAAA 601 AGAAGAAAAG AATGGGTTCG CCTGGTTAGG CGCAAAAATT TTGTGCCAGG 651 AAAACACACT TTTCTTTGTT CAAAGCACTT TGAAGCCTCC TGTTTTGACC 701 TAACAGGACA AACTCGACGA CTTAAAATGG ATGCTGTTCC AACCATTTTT 751 GATTTTTGTA CCCATATAAA GTCTATGAAA CTCAAGTCAA GGAATCTTTT 801 GAAGAAAAAC AACAGTTGTT CTCCAGCTGG ACCATCTAAT TTAAAATCAA 851 ACATTAGTAG TCAGCAAGTA CTACTTGAAC ACAGCTATGC CTTTAGGAAT 901 CCTATGGAGG CAAAAAAGAG GATCATTAAA CTGGAAAAAG AAATAGCAAG 951 CTTAAGAAGA AAAATGAAAA CTTGCCTACA AAAGGAACGC AGAGCAACTC 1001 GAAGATGGAT CAAAGCCACG TGTTTGGTAA AGAATTTAGA AGCAAATAGT 1051 GTATTACCTA AAGGTACATC AGAACACATG TTACCAACTG CCTTAAGCAG 1101 TCTTCCCTTG GAAGATTTTA AGATCCTTGA ACAAGATCAA CAAGATAAAA 1151 CACTGCTAAG TCTAAATCTA AAACAGACCA AGAGTACCTT CATTTAAATT 1201 TAGCTTGCAC AGAGCTTGAT GCCTATCCTT CATTCTTTTC AGAAGTAAAG 1251 ATAATTATGG CACTTATGCC AAAATTCATT ATTTAATAAA GTTTTACTTG 1301 AAGTAACATT ACTGAATTTG TGAAGACTTG ATTACAAAAG AATAAAAAAC 1351 TTCATATGGA AATTTTATTT GAAAATGAGT GGAAGTGCCT TACATTAGAA 1401 TTACGGACTT AAAAATTTTG CTAATAAATT GTGTGTTTGA AAGGTGTTTT 1451 TTGTTTTTGT CTTTTTAAAC TACTGTTAAA AGAACAGCTT ATGATAAGTA 1501 ATATGTTTAA CTTAGAGAAG AATTTTTTCC TGTACCAAAG TTGGCATATT 1551 GCATTCTAAA TAAGATGCTA AATAAGAGTT AACCAACATT CAACATGACC 1601 TTAAAACTGC TGGGTTTTGT ATTAATTAAA TTATAATTGG CACTGTGATT 1651 TGAAAAATTT ATAGAAAAAA AGGTACAGGG CAAGTTTTTA AATTAAAACT 1701 TTCTATATTT TGTTTTACCA GTAAAAGTGA GCTTATCATG GCCTCTCTCA 1751 TAAGAATGAT TTTAAAATAG GTTGTAAAAT ATTTTGAAAA TATTTGAATG 1801 TGAAGTACCA TTGAGTCATC CAAACTAGGT AAGGCCTCAA GTACTTTAAA 1851 CTAGTAAAAT CTAGTAGCTG ATAATATTCA CCTAAGTAAG TGTTGTAAAA 1901 TAATTCAGAG TTCAGGACCT AGCTTAGATA AATGTATACT ACTCTTTTTC 1951 TCATAGTAAA AATCTTACAT TTCCAACTTC AAAATTGGTG CTTCCATATT 2001 TGTTGATAAC CAAAACTCCT AAGGTTTTTT GTTTTCTTTT TAACTACTTT 2051 CCAAATGCAT ACTATACCTC AGAAATAGTG TATCAATATA GTGGGCTTTT 2101 TTTTTCCTCT TCATAAACCC ACAGTAAAAT TTAATCACAG GAAACTACTT 2151 ATATCTTCAC ACTTTGTATT GATAACTTAA AATGGCATCA GTTTATCTTA 2201 GACATCAGCT TGCTTTTTAT CTCCTTTTTT AGTGAGTGAA ATAGAGCAAC 2251 TAGCATGCCT GTGTTCCCAG CTACTTGGGA GGCTAAGGTG GGAAGATCAA 2301 TTGAACCTAG GAGGTTGAGG CTATAGTGAG CTGTGATTGC ACGACTGCAC 2351 TCCAGCCTGG GCAATGGAGT GAGACTCCTG TCTCTAAAAC AGCAACAACA 2401 AAAATAAAGC AACCATAGTG CATAAGGGAA ATTAAATGTT CCCTATAGAA 2451 ATATGTGTAT GTCTGTGATA GTGGTATGCA AATGCTAATT ATTTTATAAA 2501 ATAAAAGTTC AGAACTATTC TTATCATTGC CACTTGAACA ATTAAAGGGT 2551 TTGCTTTATT TCACTAATGT TTAATAGGAA CCCTTTGCTT CAAACAGCTT 2601 TGTTGAAATC ATGTAAAAAT TTGTTAATAG AGAATCAAGT TATTTAACTC 2651 AACTTATTTA ATTCAAGCTT GTGATACTAA CATACAAAGG TAGCATAAAC 2701 CAAGTCATAA ATTGCTGTAA TCTTTCCTGT AGAGTAATAG CTACTTCATG 2751 ATTTTTTTAA AAATTTCATT TTTTTGCTAT TTAGGATTGC ATTTGCTTGG 2801 CTCCTAGTAA CAATTCTTTT ACAGTATTAG CACTCTCTTT ACTAAGGAAT 2851 GCCTCCCAAG GAAATGCAAA GGTAGGAAAA GTCTCTTAGA ATGCCCATGA 2901 GGTATTTAAA ACAGATATTT ATGAAAATCT TTTTGTGAAT GTTATAAATC 2951 TTGCTAGTTA TTTTATCTTT ATCTTAAGTA TTAGATGTAG TTCCTTGGAA 3001 TTGTCATTAC ATATTTATTT TTTTCTAGTG TGGTTTCAAA TAACTTTTTG 3051 CCAACATATA ATCATCATCA AACATTCACT GACCATATCT ATTTTATAAC 3101 TCAAAATAAG TTGGACAAAT AATCATTTTA ATAAAAACTA TTTTTTCCAA 3151 GTATAACCAC TGTCATGTGG TTCACCCTTC ACCCCAGATA CAAAACACTT 3201 ATTTGTGTAG CCCAGTTCCC ATCTACAGTA ATACCTTGAA ACCTTAATAA 3251 ATTTTAAAAA TCATAAAAAT AAAATATTGT AAAATACAAC AAATTTTGGA 3301 CAAGGTTACT TCATCTTCAT TCATTATTAC CTGACAGTAT TAAACTACTA 3351 CTCAATAATT TTAGAGTAAA CTTTTCTGTG TTTTCCCCGT GATTTTCATT 3401 GTGCTGTCCT GACAACATGC TCCAAACTCT TTGCATCAAA TTGTTTTATT 3451 AACATACATT TGTCTACCTT AAAACTAGCT TTATTCACAG AGAAAGACCT 3501 AAAAGGAGTC TATTAAAATG CTGCTTTCAG TTTGATAGTT TTTTTTTTAA 3551 TCACTCTGAC CATAAACTAA CTGAAATTAT AATGGATTTT TTTTCCTCTC 3601 CCGGTCACAA CACAGATCTT CTGTTCATTT GTTCTCTGTC TACTGGGCAC 3651 CAACCTCTAC AAAGAACCAG CCAAAGGCTA GGTACTTGAT ATAAAAAGGA 3701 ATATTACATT ATTTTCTGCC CTCAAGTTGC TCTATCTCCT GAAAGAAACA 3751 AGTAATATTT ATAATACAAT ATGATAAATG CTACAAAAGA AATAGCTGTA 3801 AAGTCCTTTG GTAAATGCTG TTGAATTGGA ATTCAGTAAG AACTATAAAC 3851 TGTAGACCTT TTTATAATCA AATGCTTTTG TCTTGAAACA AAACAGATTC 3901 CTCCTTATAT TGACTTAGCA AAGGAGGTAC AAGGACATTG GCATTTGACC 3951 TGAATTATGG TGTTTTATTG AATGAGCTAT AAGACAACAT TTTTACCCTT 4001 TAAAATGAAC ACTGAACAAA TGTGTTAATG GTATCTTTGT TAAAAGGAAA 4051 ACATAGCTAT AAATAAAATA CTACATCGAA ATCCAGCACT GGAGTTCATT 4101 TGAAATTTGA TATTTTGTGT AAAGTAACAA ACCTATTAAC ACAGATTTTT 4151 AAAATAACTC AGAATCGTAT AAAGCACTTT GGTACTTATT TGTTCTCTTT 4201 TCCCTTACAT TCTGTGTGGT AGGTGGTATT ATCTCTGATT TACACATGAA 4251 GACATCCTTG TTAATGCAAT TTATTTATTC ATTCGGGCAT TTACTGTGTG 4301 CCAACTTGCA AAAGGAATAG AAATGTCTGT GATCTAGATA GTTCTAGATT 4351 GAACATAGAT TTTCTGCCAA CAAATCCTCT CTGCTGTTCA CATTATCCTT 4401 TGTTTAACGT ATGAACCAGG TTACTAAAAT AGGATAAATC ATGTGTCTTA 4451 GAATATGAAA ATAGTAAGGT CTTTGAGGTC ACTTGATCTT CTCTAAGTAG 4501 ACTTTATAAT ATTGTGTTTT ATCTCATTTC TCAATATTAG AATACGGGTA 4551 GATTTTAATT TTGCTATAAT ATAGGAAATG GTTCATCTTT GTACCAAAAT 4601 ATTGCATTCT TCTGATATTT AGACAGTTGG AAACTTTCTA AAATTGAGGA 4651 TTTTGTAGTG TATACTAAAT AATTGCATAT TCAAAAAAAT GTATTCTGAG 4701 TATGGTGATA TTAAACATTT TTCCCCAAAA AAAAAAAAAA AAAAAAAA BLAST Results No BLAST result Medline entries 98107671: Regulation of mterferon-induced protein kinase PKR: modulation of P58IPK inhibitory function by a novel protein, P52rIPK Peptide information for frame 1 ORF from 511 bp to 1194 bp; peptide length: 228 Category: similarity to known protein 1 MPTNCAAAGC ATTYNKHINI ΞFHRFPLDPK RRKEWVRLVR RKNFVPGKHT 51 FLCΞKHFEAS CFDLTGQTRR LKMDAVPTIF DFCTHIKSMK LKSRNLLKKN 101 NSCSPAGPSN LKSNISSQQV LLEHSYAFRN PMEAKKRIIK LEKEIASLRR 151 KMKTCLQKER RATRRWIKAT CLVKNLEANS VLPKGTSEHM LPTALSSLPL 201 EDFKILEQDQ QDKTLLSLNL KQTKSTFI BLASTP hits Entry AF007393_1 from database TREMBL: product: "P52rIPK"; Homo sapiens P52rIPK mRNA, complete eds. Score = 166, P = 2.5e-ll, identities = 40/106, positives = 56/106 Alert BLASTP hits for DKFZphfbr2_22ι4, frame 1 No Alert BLASTP hits found Pedant information for DKFZphfbr2_22ι4, frame 1 Report for DKFZphfbr2_22ι4.1 [LENGTH] 228 [MW] 26259.94 [pi] 10.17 [HOMOL] TREMBL:AF007393_1 product: 'P52rIPK"; Homo sapiens P52rIPK mRNA, complete eds. le-09 [PROSITE] MYRISTYL 1 [PROSITE] CAMP_PHOΞPHO_SITE 1 [PROSITE] CK2_PHOSPHO_SITE 2 [PROSITE] PKC_PHOSPHO_SITE 4 [PROSITE] ASN_GLYCOSYLATION 3 [KW] All_Alpha [KW] LOW COMPLEXITY 7.02 % SEQ MPTNCAAAGCATTYNKHINISFHRFPLDPKRRKEWVRLVRRKNFVPGKHTFLCSKHFEAS SEG PRD cccccccccccccccccccceeeecccccchhhhhhhhhhhhhcccccceeehhhhhhhh SEQ CFDLTGQTRRLKMDAVPTIFDFCTHIKSMKLKSRNLLKKNNSCSPAGPSNLKSNISSQQV SEG xxxxxxxxxxxxxxxx PRD cccccccccccccccccceeeeccccchhhhhhhhhhhccccccccccccccccccchhh SEQ LLEHSYAFRNPMEAKKRIIKLEKEIASLRRKMKTCLQKERRATRRWIKATCLVKNLEANS SEG PRD hhhhcccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhheeeecccccc SEQ VLPKGTSEHMLPTALSSLPLEDFKILEQDQQDKTLLSLNLKQTKSTFI SEG PRD cccccccccccccccccccccchhhhhhcccccccccccccccccccc Prosite for DKFZphfbr2_22ι4.1 PS00001 19->23 ASN GLYCOSYLATION PDOC00001 PS00001 100->104 ASN GLYCOSYLATION PDOC00001 PS00001 114-M18 ASN GLYCOSYLATION PDOC00001 PS00004 160->164 CAMP PHOSPHO SITE PDOC00004 PS00005 68->71 PKC PHOSPHO SITE PDOC00005 PS00005 88->91 PKC PHOSPHO SITE PDOC00005 PS00005 147->150 PKC PHOSPHO SITE PDOC00005 PΞ00005 163->166 PKC PHOSPHO SITE PDOC00005 PS00006 60->64 CK2 PHOSPHO SITE PDOC00006 PS00006 78->82 CK2 PHOSPHO SITE PDOC00006 PS00008 9->15 MYRISTYL PDOC00008 (No Pfam data available for DKFZphfbr2 22ι4.1) DKFZphfbr2_22k3 group: brain derived DKFZphfbr2_22k3 encodes a novel 538 amino acid protein with weak similarity to extensins. No informative BLAST results; no predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of brain-specific genes . weak similarity to extensins complete cDNA, complete eds, few EST hits CpG Island in 5' UTR complete cDNA Sequenced by AGOWA Locus : unknown Insert length: 2775 bp Poly A stretch at pos. 2755, polyadenylation signal at pos. 2718 1 GGGGCTGCCC GCGCGCTCCA CGGTGCAGAG CTCTAAGCGC GCGGGCTGGC 51 AGGCTGCGGC GCGTCAAGGT CAGCCTGGAG CTGGGTGGCG GCCTGCCTGG 101 GGGCGGGGGA CCCTACTGGA GGCCCGGGCT GGGGCCTCCC AGCGCCTCGG 151 CCATATTGAA TAGCTTCGAC TGGACCGTCT TTGTCTGCGA AGTCCTGTCC 201 CAAGTTCCAG CCGCGTCCCT GGGGCCTGGG GCAGGAAGAG TCGCTGGCAG 251 CCCGCGCGCC CCAACTTGGA GCTGGGACAC CACGTTTCCA GCTTGGAGTG 301 GGCCTTGAGC CTTGGGACTG ACCTCGCCCC CGGCTCACGT AGGCATCCTG 351 GAAATTGATT CCCCCAAGTC CTTGGTGGGG GAGCCGGACT TGGTCAAGAC 401 TGTACTTGTT GCAGGCGAAG AGATTGGAGG CGTTTGGCTC GTCCCTGGCT 451 AGGGAGGTGA GACTCTCCGG TCAGCGTTGC TGGAACTCCC CCCATCCAGT 501 CCCTCCCTCA AGACTAAGGG CTACAGTAGT TTGTTGGGGC TCATTGCCCC 551 CTCACCCCAG ATATCACCCT GGAGATCTTA AAGACTCTCG AGAAAAGCCA 601 CGTGGGGGGC TGGTTCCCCT GGGGCTTCCT GCCGTCCCCC GACTGCCTCA 651 TTCTTTGGAG CGTCCCCGAT GTCTGCAAAG ATGTGGATTT GGACGTCCTC 701 GTGGAAGCCC TAAAGCCCGT GGGGACATTT AAGAAGATCG GCAAGGTGTT 751 CCGCAAGGAG GAGGACTCCA CGGTGGGGAT GCTGCAGATC GGGGAGGACG 801 TCGACTATTT GCTCATCCCC CGGGAGGTCA GGCTGGCTGG GGGCGTCTGG 851 AGAGTCATCT CTAAGCCCGC CACCAAGGAA GCAGAATTTC GGGAGCGGCT 901 GACCCAGTTC CTGGAAGAAG AGGGCCGCAC CCTGGAGGAC GTGGCCCGCA 951 TCATGGAGAA GAGCACCCCG CACCCGCCCC AGCCCCCCAA AAAGCCCAAG 1001 GAGCCCCGAG TGAGGAGGAG AGTGCAGCAG ATGGTGACTC CTCCGCCCCG 1051 GCTGGTCGTG GGCACGTACG ACAGCAGCAA CGCCAGCGAC AGCGAGTTCA 1101 GCGACTTCGA GACCTCCAGA GACAAGAGCC GCCAGGGCCC GCGGCGGGGC 1151 AAGAAGGTGC GCAAAATGCC CGTCAGCTAC CTGGGCAGCA AGTTCCTGGG 1201 AAGCGACCTG GAGAGTGAGG ATGATGAGGA ACTGGTCGAG GCCTTCCTCC 1251 GGCGACAGGA GAAGCAGCCC AGCGCGCCGC CTGCCCGCCG CCGCGTCAAC 1301 CTGCCAGTGC CCATGTTTGA GGACAACCTG GGGCCTCAGC TGTCCAAAGC 1351 GGACAGGTGG CGGGAGTATG TCAGCCAGGT GTCCTGGGGG AAGCTGAAGC 1401 GGAGGGTGAA GGGTTGGGCG CCGAGGGCGG GCCCCGGGGT GGGCGAGGCC 1451 CGGCTGGCCT CCACCGCAGT GGAGAGCGCA GGGGTATCAT CGGCGCCAGA 1501 GGGCACCAGC CCGGGGGATC GCTTGGGAAA CGCGGGAGAT GTTTGTGTGC 1551 CCCAGGCTTC CCCTAGGCGA TGGAGGCCCA AGATCAACTG GGCCTCCTTT 1601 CGGCGCCGCA GGAAGGAGCA GACAGCACCC ACAGGTCAGG GGGCAGACAT 1651 CGAGGCTGAT CAGGGGGGAG AGGCTGCAGA TAGTCAAAGG GAAGAGGCCA 1701 TAGCTGACCA GCGGGAAGGG GCTGCAGGTA ATCAGAGGGC TGGGGCCCCA 1751 GCTGACCAGG GGGCAGAGGC TGCAGATAAT CAGAGGGAAG AGGCTGCAGA 1801 TAATCAGAGG GCAGGGGCCC CAGCTGAGGA GGGGGCAGAG GCTGCAGATA 1851 ACCAGAGGGA AGAGGCTGCA GATAATCAGA GGGCAGAGGC CCCAGCTGAC 1901 CAGAGGTCAC AGGGCACAGA TAACCACAGG GAAGAGGCTG CAGATAATCA 1951 GAGGGCGGAG GCCCCAGCTG ACCAGGGGTC AGAGGTTACA GATAATCAAA 2001 GGGAAGAGGC CGTACATGAC CAGAGGGAAA GGGCCCCAGC TGTCCAGGGT 2051 GCAGATAATC AGAGGGCACA GGCCCGGGCT GGCCAGAGGG CAGAGGCTGC 2101 ACATAATCAG AGGGCAGGGG CCCCAGGTAT CCAGGAAGCT GAAGTCTCAG 2151 CTGCCCAAGG GACCACAGGA ACAGCTCCAG GAGCCAGGGC CCGGAAACAG 2201 GTCAAGACAG TGAGGTTCCA GACCCCTGGA CGCTTTTCGT GGTTTTGCAA 2251 GCGCCGGAGA GCCTTCTGGC ACACTCCCCG GTTGCCAACC CTGCCCAAGA 2301 GAGTCCCCAG GGCAGGAGAG GTCAGGAACC TCAGGGTGCT GAGGGCCGAG 2351 GCCAGAGCAG AAGCTGAGCA GGGAGAGCAA GAAGACCAGC TGTGAGGTGA 2401 GGGCTAGAGA CAGCCCACGG GCCCTCCCTC CAAGTGTGGG AGGGAGAGAT 2451 GCTCTGCCTC TGAACTTCAA AGTGGAGGTG GAGTGCTGGC CACGTCTCCA 2501 CCTAACAACC CTCTTTATTC TCTTGTTAAA GTTTTGTTCA TGCTTTGATT 2551 TTTTTTTAAA TTTTTTAGAG ACAGGGTCTC ACTCTGTTGC CCAGGCTGGA 2601 GTGCAGTGGC ATGATCATAA CTCACTGCAG CCTCAAACTT CTGGCCTCAA 2651 GTGATCCTCC TGCCTCGGCC TCCCAAAATG CTGGGATTAC AGATGTGAGC 2701 CACCACACAC ACCATCTGAT TAAAAAAAAA AAATACTGAT TCCCTGTAGC 2751 AACCCAAAAA AAAAAAAAAA AAAAA BLAST Results Entry HS164A7F from database EMBL: H. sapiens CpG island DNA genomic Msel fragment, clone 164a7, forward read cpgl64a7. ftla . Score = 740, P = 3.0e-25, identities = 150/151 Medlme entries No Medlme entry Peptide information for frame 2 ORF from 779 bp to 2392 bp; peptide length: 53£ Category: similarity to known protein 1 MLQIGEDVDY LLIPREVRLA GGVWRVISKP ATKEAEFRER LTQFLEEEGR 51 TLEDVARIME KSTPHPPQPP KKPKEPRVRR RVQQMVTPPP RLVVGTYDSS 101 NASDSEFSDF ETSRDKSRQG PRRGKKVRKM PVSYLGSKFL GSDLESEDDE 151 ELVEAFLRRQ EKQPSAPPAR RRVNLPVPMF EDNLGPQLSK ADRWREYVSQ 201 VSWGKLKRRV KGWAPRAGPG VGEARLASTA VESAGVSSAP EGTSPGDRLG 251 NAGDVCVPQA SPRRWRPKIN WASFRRRRKE QTAPTGQGAD IEADQGGEAA 301 DSQREEAIAD QREGAAGNQR AGAPADQGAE AADNQREEAA DNQRAGAPAE 351 EGAEAADNQR EEAADNQRAE APADQRSQGT DNHREEAADN QRAEAPADQG 401 SEVTDNQREE AVHDQRERAP AVQGADNQRA QARAGQRAEA AHNQRAGAPG 451 IQEAEVSAAQ GTTGTAPGAR ARKQVKTVRF QTPGRFSWFC KRRRAFWHTP 501 RLPTLPKRVP RAGEVRNLRV LRAEARAEAE QGEQEDQL BLASTP hits Entry RNU67136_1 from database TREMBL: "A-kinase anchoring protein AKAP150"; Rattus norvegicus A-kinase anchoring protein AKAP150 mRNA, complete eds. Rattus norvegicus (Norway rat) Length = 714 Score = 182 (64.1 bits), Expect = 1.2e-10, P = 1.2e-10 Identities = 73/257 (28%), Positives = 104/257 (40%) Alert BLASTP hits for DKFZphfbr2_22k3, frame 2 TREMBL :PFSANTY_1 product: "S-antigen"; Plasmodium falciparum KF1916 S-antigen gene, complete eds., N = 1, Score = 178, P = 3.7e-ll >TREMBL:PFSANTY_1 product: "S-antigen"; Plasmodium falciparum KF1916 S-antigen gene, complete eds. Length = 285 HSPs: Score = 178 (26.7 bits). Expect = 3.7e-ll, P = 3.7e-ll Identities = 60/217 (27%), Positives = 97/217 (44%) Query: 269 INWASFRRRRKEQTAPTGQGA-DIEADQGGEAADSQRE-EAIADQ REGAAGNQRAGA 323 +N + + + E G+G D E E +D+ E E I Q E A N+ AG+ Sbjct: 47 LNGKNGKGNKYEDLQEEGEGENDDEEHSNSEESDNDEENEIIVGQDGSNEKAGSNEEAGS 106 Query: 324 PADQGAEAADNQREEAADNQRAGAPAEEGA—EAADNQR EEAADNQRAEAPADQRS 377 G+ E+A N++AG+ E G+ EA N+ EEA N++A + S Sbjct: 107 NEKAGSNEEAGSNEKAGSNEKAGSNEEAGSNEEAGSNEEAGSNEEAGSNEKAGSNEKAGS 166 Query: 378 QGTDNHREEAADNQRAEAPADQGSEVTDNQREEAVHDQRERAPAVQGADNQRAQAR—AG 435 EEA N++A + + GS E+A +++ + G+ N++A + AG Sbjct: 167 NEKAGSNEEAGSNEKAGSNEEAGSNEKAGSNEKAGSNEKAGSNEEAGS-NEKAGSNEEAG 225 Query: 436 QRAEAAHNQRAGA PGIQEAEVSAAQGTTGTA-PGA 469 EA N+ AG+ G E + +G GT PG+ Sbjct: 226 SNEEAGSNEEAGSNEEAGSNEGSEAGTEGPKGTGGPGS 263 Score = 173 (26.0 bits), Expect = 1.5e-10, P = 1.5e-10 Identities = 51/190 (26%), Positives = 83/190 (43%) Query: 279 KEQTAPTGQ-GADIEADQGGEAADSQREEAIADQREGAAGNQRAGAPADQGAEAADNQRE 337 +E GQ G++ +A EA +++ A E A N++AG+ G+ E Sbjct: 83 EENEIIVGQDGSNEKAGSNEEAGSNEK AGSNEEAGSNEKAGSNEKAGSNEEAGSNE 138 Query: 338 EAADNQRAGAPAEEGAEAADNQREEAADNQRAEAPADQRSQGTDNHREEAADNQRAEAPA 397 EA N+ AG+ E G+ E+A N++A + + S EEA N++A + Sbjct: 139 EAGSNEEAGSNEEAGSNEKAGSNEKAGSNEKAGSNEEAGSNEKAGSNEEAGSNEKAGSNE 198 Query: 398 DQGSEVTDNQREEAVHDQRERAPAVQGADNQRAQARAGQRAEAAHNQRAGAPGIQEAEVS 457 GS EEA +++ + G++ + AG EA N+ AG+ EA Sbjct: 199 KAGSNEKAGSNEEAGSNEKAGSNEEAGSNEE AGSNEEAGSNEEAGSNEGSEAGTE 253 Query: 458 AAQGTTGTAPG 468 +GT G G Sbjct: 254 GPKGTGGPGSG 264 Score = 147 (22.1 bits), Expect = 1.6e-07, P = 1.6e-07 Identities = 40/168 (23%), Positives = 70/168 (41%) Query: 288 GADIEADQGGEAADSQR—EEAIADQREGAAGNQRAGAPADQGAEAADNQREEAADNQRA 345 G++ EA +A +++ A E A N+ AG+ + G+ E+A N++A Sbjct: 111 GSNEEAGSNEKAGSNEKAGΞNEEAGSNEEAGSNEEAGSNEEAGSNEKAGSNEKAGSNEKA 170 Query: 346 GAPAEEGAEAADNQREEAADNQRAEAPADQRSQGTDNHREEAADNQRAEAPADQGSEVTD 405 G+ E G+ EEA N++A + S EEA N++A + + GS Sbjct: 171 GSNEEAGSNEKAGSNEEAGSNEKAGSNEKAGSNEKAGSNEEAGSNEKAGSNEEAGSNEEA 230 Query: 406 NQREEAVHDQR—ERAPAVQGADNQRAQARAGQRAEAAHNQRAGAPGI 451 EEA ++ + G + + G E +HN++ I Sbjct: 231 GSNEEAGSNEEAGSNEGSEAGTEGPKGTGGPGSGGEHSHNKKKSKKSI 278 Score = 101 (15.2 bits), Expect = 2.5e-02, P = 2.4e-02 Identities = 26/100 (26%), Positives = 47/100 (47%) Query: 281 QTAPTGQGADIEADQGGEAADSQREEAIADQREGAAGNQRAGAPADQGAEAADNQREEAA 340 + A + + A + G EEA ++++ G+ N++AG+ G+ E+A Sbjct: 162 EKAGSNEKAGSNEEAGSNEKAGSNEEAGSNEKAGS--NEKAGSNEKAGSNEEAGSNEKAG 219 Query: 341 DNQRAGAPAEEGAEAADNQREEAADNQRAEAPADQRSQGT 380 N+ AG+ E G+ EEA N+ +EA + +GT Sbjct: 220 SNEEAGΞNEEAGSNEEAGSNEEAGSNEGSEA-GTEGPKGT 258 Pedant information for DKFZphfbr2_22k3, frame 2 Report for DKFZphfbr2_22 3.2 [LENGTH] 538 [MW] 59402.19 [pi] 8.72 [HOMOL] TREMBL:AF037364_1 gene: "MAI"; product: "paraneoplastic neuronal antigen MAI" Homo sapiens paraneoplastic neuronal antigen MAI (MAI) mRNA, complete eds. 4e-10 [PROSITE] AMIDATION 1 [PROSITE] MYRISTYL 12 [PROSITE] CK2_PHOSPHO_SITE 11 [PROSITE] PKC_PHOSPHO_SITE 6 [PROSITE] ASN_GLYCOSYLATION 1 [KW] All_Alpha [KW] LOW_COMPLEXITY 18.03 % SEQ MLQIGEDVDYLLIPREVRLAGGVWRVISKPATKEAEFRERLTQFLEEEGRTLEDVARIME SEG PRD cccccccccccccccccccccceeeeeeecccchhhhhhhhhhhhhhhccchhhhhhhhh SEQ KSTPHPPQPPKKPKEPRVRRRVQQMVTPPPRLVVGTYDSΞNASDSEFΞDFETΞRDKSRQG SEG xxxxxxxxxxxxxxxxxxx PRD hcccccccccccccccchhhhhhhhhccccceeeeecccccccccccccccccccccccc SEQ PRRGKKVRKMPVSYLGSKFLGSDLESEDDEELVEAFLRRQEKQPSAPPARRRVNLPVPMF SEG xxxxxxxxxxx PRD ccccccccccceeeccccccccccccchhhhhhhhhhhhhhccccccchhhhhccccccc SEQ EDNLGPQLSKADRWREYVSQVSWGKLKRRVKGWAPRAGPGVGEARLASTAVESAGVSSAP SEG PRD cccccccchhhhhhhhhheeeeccchhhhhhccccccccccchhhhhhhhhhhccccccc SEQ EGTSPGDRLGNAGDVCVPQAΞPRRWRPKINWASFRRRRKEQTAPTGQGADIEADQGGEAA SEG PRD cccccccccccccceeeecccccccccccchhhhhhhhhhhhhcccccchhhhhccchhh SEQ DSQREEAIADQREGAAGNQRAGAPADQGAEAADNQREEAADNQRAGAPAEEGAEAADNQR SEG xxxxxxxxxxxxx xxxxxxxxxxxx.... PRD hhhhhhhhhhhhhhhhhhhhhccccchhhhhhhhhhhhhhhhhhccccchhhhhhhhhhh SEQ EEAADNQRAEAPADQRSQGTDNHREEAADNQRAEAPADQGSEVTDNQREEAVHDQRERAP SEG PRD hhhhhhhhhhhhhhhhhcccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh SEQ AVQGADNQRAQARAGQRAEAAHNQRAGAPGIQEAEVSAAQGTTGTAPGARARKQVKTVRF SEG xxxxxxxxxxxxxx xxxxxxxxxxxxxx PRD hhccchhhhhhhhhhhhhhhhhhhhhhccchhhhhhhhhhccccccccchhhhhhhhhhh SEQ QTPGRFSWFCKRRRAFWHTPRLPTLPKRVPRAGEVRNLRVLRAEARAEAEQGEQEDQL SEG xxxxxxxxxxxxxx ... PRD cccccceeehhhhhhhccccccccccccccccccchhhhhhhhhhhhhhhhhhhhccc Prosite for DKFZphfbr2_22k3.2 PS00001 101->105 ASN_GLYCOSYLATION PDOC00001 PS00005 112->115 PKC_PHOSPHO_ΞITE PDOC00005 PS00005 261->264 PKC_PHOSPHO_SITE PDOC00005 PS00005 2 73->276 PKC_PHOSPHO_SITE PDOC00005 PS00005 302->305 PKC_PHOSPHO_SITE PDOC00005 PS00005 477->480 PKC_PHOSPHO_SITE PDOC00005 PS00005 499->502 PKC_PHOSPHO_SITE PDOC00005 PS00006 51->55 CK2_PHOΞPHO_SITE PDOC00006 PS00006 103->107 CK2_PHOSPHO_SITE PDOC00006 PS00006 108->112 CK2_PHOΞPHO_SITE PDOC00006 PS00006 112->116 CK2_PHOSPHO_SITE PDOC00006 PS00006 142->146 CK2_PHOSPHO_SITE PDOC00006 PS00006 146->150 CK2_PHOSPHO_SITE PDOC00006 PS00006 189->193 CK2_PHOSPHO_SITE PDOC00006 PS00006 229->233 CK2_PHOSPHO_SITE PDOC00006 PS00006 238->242 CK2_PHOSPHO_SITE PDOC00006 PS00006 244->248 CK2_PHOSPHO_SITE PDOC00006 PS00006 302->306 CK2_PHOSPHO_SITE PDOC00006 PS00008 95->101 MYRISTYL PDOC00008 PS00008 220->226 MYRISTYL PDOC00008 PS00008 242->248 MYRISTYL PDOC00008 PΞ00008 296->30 2 MYRISTYL PDOC00008 PS00008 314->320 MYRISTYL PDOC00008 PS00008 317->323 MYRISTYL PDOC00008 PS00008 328->334 MYRISTYL PDOC00008 PS00008 352->358 MYRISTYL PDOC00008 PS00008 400->406 MYRISTYL PDOC00008 PS00008 450->456 MYRISTYL PDOC00008 PS00008 461->467 MYRISTYL PDOC00008 PS00008 464->470 MYRISTYL PDOC00008 PS00009 123-M27 AMIDATION PDOC00009 (No Pfam data available for DKFZphfbr2_22k3.2) DKFZphfbr2_22k8 group: brain derived DKFZphfbr2_22k8 encodes a novel 172 amino acid protein without similarity to known proteins. No informative BLAST results; no predictive prosite, pfam or SCOP motife The new protein can find application studying the expression profile of bram-specific genes . unknown complete cDNA, complete eds, EST hits Sequenced by AGOWA Locus: /map="7" Insert length: 2789 bp Poly A stretch at pos. 2769, polyadenylation signal at pos. 2756 1 GGGGGAGCCA TGAGGCGCCA GCCTGCGAAG GTGGCGGCGC TGCTGCTCGG 51 GCTGCTCTTG GAGTGCACAG AAGCCAAAAA GCATTGCTGG TATTTCGAAG 101 GACTCTATCC AACCTATTAT ATATGCCGCT CCTACGAGGA CTGCTGTGGC 151 TCCAGGTGCT GTGTGCGGGC CCTCTCCATA CAGAGGCTGT GGTACTTCTG 201 GTTCCTTCTG ATGATGGGCG TGCTTTTCTG CTGCGGAGCC GGCTTCTTCA 251 TCCGGAGGCG CATGTACCCC CCGCCGCTGA TCGAGGAGCC AGCCTTCAAT 301 GTGTCCTACA CCAGGCAGCC CCCAAATCCC GGCCCAGGAG CCCAGCAGCC 351 GGGGCCGCCC TATTACACTG ACCCAGGAGG ACCGGGGATG AACCCTGTCG 401 GGAATTCCAC GGCAATGGCT TTCCAGGTCC CACCCAACTC ACCCCAGGGG 451 AGTGTGGCCT GCCCGCCCCC TCCAGCCTAC TGCAACACGC CTCCGCCCCC 501 GTACGAACAG GTAGTGAAGG CCAAGTAGTG GGGTGCCCAC GTGCAAGAGG 551 AGAGACAGGA GAGGGCCTTT CCCTGGCCTT TCTGTCTTCG TTGATGTTCA 601 CTTCCAGGAA CGGTCTCGTG GGCTGCTAAG GGCAGTTCCT CTGATATCCT 651 CACAGCAAGC ACAGCTCTCT TTCAGGCTTT CCATGGAGTA CAATATATGA 701 ACTCACACTT TGTCTCCTCT GTTGCTTCTG TTTCTGACGC AGTCTGTGCT 751 CTCACATGGT AGTGTGGTGA CAGTCCCCGA GGGCTGACGT CCTTACGGTG 801 GCGTGACCAG ATCTACAGGA GAGAGACTGA GAGGAAGAAG GCAGTGCTGG 851 AGGTGCAGGT GGCATGTAGA GGGGCCAGGC CGAGCATCCC AGGCAAGCAT 901 CCTTCTGCCC GGGTATTAAT AGGAAGCCCC ATGCCGGGCG GCTCAGCCGA 951 TGAAGCAGCA GCCGACTGAG CTGAGCCCAG CAGGTCATCT GCTCCAGCCT 1001 GTCCTCTCGT CAGCCTTCCT CTTCCAGAAG CTGTTGGAGA GACATTCAGG 1051 AGAGAGCAAG CCCCTTGTCA TGTTTCTGTC TCTGTTCATA TCCTAAAGAT 1101 AGACTTCTCC TGCACCGCCA GGGAAGGATA GCACGTGCAG CTCTCACCGC 1151 AGGATGGGGC CTAGAATCAG GCTTGCCTTG GAGGCCTGAC AGTGATCTGA 1201 CATCCACTAA GCAAATTTAT TTAAATTCAT GGGAAATCAC TTCCTGCCCC 1251 AAACTGAGAC ATTGCATTTT GTGAGCTCTT GGTCTGATTT GGAGAAAGGA 1301 CTGTTACCCA TTTTTTTGGT GTGTTTATGG AAGTGCATGT AGAGCGTCCT 1351 GCCCTTTGAA ATCAGACTGG GTGTGTGTCT TCCCTGGACA TCACTGCCTC 1401 TCCAGGGCAT TCTCAGGCCC GGGGGTCTCC TTCCCTCAGG CAGCTCCAGT 1451 GGTGGGTTCT GAAGGGTGCT TTCAAAACGG GGCACATCTG GCCGGGAAGT 1501 CACATGGACT CTTCCAGGGA GAGAGACCAG CTGAGGCGTC TCTCTCTGAG 1551 GTTGTGTTGG GTCTAAGCGG GTGTGTGCTG GGCTCCAAGG AGGAGGAGCT 1601 TGCTGGGAAA AGACAGGAGA AGTACTGACT CAACTGCACT GACCATGTTG 1651 TCATAATTAG AATAAAGAAG AAGTGGTCGG AAATGCACAT TCCTGGATAG 1701 GAATCACAGC TCACCCCAGG ATCTCACAGG TAGTCTCCTG AGTAGTTGAC 1751 GGCTAGCGGG GAGCTAGTTC CGCCGCATAG TTATAGTGTT GATGTGTGAA 1801 CGCTGACCTG TCCTGTGTGC TAAGAGCTAT GCAGCTTAGC TGAGGCGCCT 1851 AGATTACTAG ATGTGCTGTA TCACGGGGAA TGAGGTGGGG GTGCTTATTT 1901 TTTAATGAAC TAATCAGAGC CTCTTGAGAA ATTGTTACTC ATTGAACTGG 1951 AGCATCAAGA CATCTCATGG AAGTGGATAC GGAGTGATTT GGTGTCCATG 2001 CTTTTCACTC TGAGGACATT TAATCGGAGA ACCTCCTGGG GAATTTTGTG 2051 GGAGACACTT GGGAACAAAA CAGACACCCT GGGAATGCAG TTGCAAGCAC 2101 AGATGCTGCC ACCAGTGTCT CTGACCACCC TGGTGTGACT GCTGACTGCC 2151 AGCGTGGTAC CTCCCATGCT GCAGGCCTCC ATCTAAATGA GACAACAAAG 2201 CACAATGTTC ACTGTTTACA ACCAAGACAA CTGCGTGGGT CCAAACACTC 2251 CTCTTCCTCC AGGTCATTTG TTTTGCATTT TTAATGTCTT TATTTTTTGT 2301 AATGAAAAAG CACACTAAGC TGCCCCTGGA ATCGGGTGCA GCTGAATAGG 2351 CACCCAAAAG TCCGTGACTA AATTCCGTTT GTCTTTTTGA TAGCAAATTA 2401 TGTTAAGAGA CAGTGATGGC TAGGGCTCAA CAATTTTGTA TTCCCATGTT 2 451 TGTGTGAGAC AGAGTTTGTT TTCCCTTGAA CTTGGTTAGA ATTGTGCTAC 2 501 TGTGAACGCT GATCCTGCAT ATGGAAGTCC CACTTTGGTG ACATTTCCTG 2551 GCCATTCTTG TTTCCATTGT GTGGATGGTG GGTTGTGCCC ACTTCCTGGA 2601 GTGAGACAGC TCCTGGTGTG TAGAATTCCC GGAGCGTCCG TGGTTCAGAG 2651 TAAACTTGAA GCAGATCTGT GCATGCTTTT CCTCTGCAGC AATTGGCTCG 2701 TTTCTCTTTT TTGTTCTCTT TTGATAGGAT CCTGTTTCCT ATGTGTGCAA 2751 AATAAAAATA AATTTGGGCA AAAAAAAAAA AAAAAAAAA BLAST Results Entry HS671255 from database EMBL: human STS SHGC-11828. Length = 400 Minus Strand HSPs: Score = 1822 (273.4 bits), Expect = 4.8e-76, P = 4.8e-76 Identities = 382/397 (96%), Positives = 382/397 (96%), Medlme entries No Medlme entry Peptide information for frame 1 ORF from 10 bp to 525 bp; peptide length: 172 Category: putative protein Classification: unset 1 MRRQPAKVAA LLLGLLLECT EAKKHCWYFE GLYPTYYICR SYEDCCGSRC 51 CVRALSIQRL WYFWFLLMMG VLFCCGAGFF IRRRMYPPPL IEEPAFNVSY 101 TRQPPNPGPG AQQPGPPYYT DPGGPGMNPV GNSTAMAFQV PPNSPQGSVA 151 CPPPPAYCNT PPPPYEQVVK AK BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_22k8, frame 1 PIR:S14970 extensm class I (clone wl7-l) - tomato, N = 1, Score = 118, P = 2.3e-07 >PIR:S14970 extensin class I (clone wl7-l) - tomato Length = 132 HSPs: Score = 118 (17.7 bits), Expect = 2.3e-07, P = 2.3e-07 Identities = 30/82 (36%), Positives = 35/82 (42%) Query: 87 PPPLIEEPAFNVSYTRQPPNPGPGAQQPGPPYYTDPGGPGMNPVGNSTAMAFQVPPNSPQ 146 PPP P Y + PP P P P P YY P P +P + P SP Sbjct: 32 PPPSPSPPP—PYYYKSPPPPSPSP—PPPYYYKSPPPPDPSPPPPYYYKSPPPPSPSPP 87 Query: 147 GSVACPPPPAYCNTPPPP— EQV 168 PPPP Y + PPPP YE + Sbjct: 88 PPSPSPPPPTYSSPPPPPPFYENI 111 Score = 104 (15.6 bits), Expect = 6.9e-06, P = 6.9e-06 Identities = 28/78 (35%), Positives = 34/78 (43%) Query: 87 PPPLIEEPAFNVSYTRQPPNPGPGAQQPGPPYYTDPGGPGMNPVGNSTAMAFQVPPNSPQ 146 PP P + Y + PP P P P P YY P P +P ++ PP P Sbjct: 1 PPSPSPPPPY YYKΞPPPPSPSP—PPPYYYKSPPPPSPSP PPPYYYKSPP-PPS 51 Query: 147 GSVACPPPPAYCNTPPPP 164 S PPPP Y +PPPP Sbjct: 52 PS PPPPYYYKSPPPP 66 Score = 102 (15.3 bits), Expect = l.le-05, P = l.le-05 Identities = 30/78 (38%), Positives = 33/78 (42%) Query: 87 PPPLIEEPAFNVSYTRQPPNPGPGAQQPGPPYYTDPGGPGMNPVGNSTAMAFQVPPNSPQ 146 PPP P Y + PP P P P P YY P P +P S + PP P Sbjct: 48 PPPSPSPPP—PYYYKSPPPPDPSP—PPPYYYKSPPPPSPSPPPPSPS PP-PPT 97 Query: 147 GSVACPPPPAYCNTPPPP 164 S PPPP Y N P PP Sbjct: 98 YSSPPPPPPFYENIPLPP 115 Score = 95 (14.3 bits), Expect = 2.4e-04, P = 2.4e-04 Identities = 24/61 (39%), Positives = 29/61 (47%) Query 104 PPNPGPGAQQPGPPYYTDPGGPGMNPVGNSTAMAFQVPPNSPQGSVACPPPPAYCNTPPP 163 PP+P P P P YY P P +P ++ PP P S PPPP Y +PPP Sbjct 1 PPSPSP PPPYYYKSPPPPSPSP PPPYYYKSPP-PPSPS PPPPYYYKSPPP 49 Query 164 P 164 P Sbjct 50 P 50 Score = 68 (10.2 bits), Expect = 4.2e+00, P = 9.8e-01 Identities = 24/69 (34%), Positives = 29/69 (42%) Query: 87 PPPLIEEPAFNVSYTRQPP NPGPGAQQPGPPYYTDPGGPGMNPVGNSTAMAFQVPPN 143 PPP P Y PP +P P + P PP Y+ P P P + + PP Sbjct: 63 PPPPDPSPPPPYYYKSPPPPSPSPPPPSPSPPPPTYSSPPPPP—PFYENIPL PPV 116 Query: 144 SPQGSVACPPPP 155 S A PPPP Sbjct: 117 IGV-SYASPPPP 127 Peptide information for frame 3 ORF from 0 bp to 368 bp; peptide length: 123 Category: questionable ORF Classification: unset 1 GSHEAPACEG GGAAARAALG VHRSQKALLV FRRTLSNLLY MPLLRGLLWL 51 QVLCAGPLHT EAVVLLVPSD DGRAFLLRSR LLHPEAHVPP AADRGASLQC 101 VLHQAAPKSR PRSPAAGAAL LH BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_22k8, frame 3 No Alert BLASTP hits found Pedant information for DKFZphfbr2_22k8, frame 1 Report for DKFZphfbr2_22k8.1 [LENGTH] 172 [MW] 19194.47 [pi] 8.77 [KW] SIGNAL_PEPTIDE 23 [KW] TRANSMEMBRANE 1 [KW] LOW_COMPLEXITY 27.33 % SEQ MRRQPAKVAALLLGLLLECTEAKKHCWYFEGLYPTYYICRSYEDCCGSRCCVRALΞIQRL SEG xxxxxxx PRD ccchhhhhhhhhhhhhhhhhhhhhhcccccccccceeeeccccccccccchhhhhhhhhh MEM SEQ WYFWFLLMMGVLFCCGAGFFIRRRMYPPPLIEEPAFNVSYTRQPPNPGPGAQQPGPPYYT SEG xxxxxxxxxxxxxxxxxx PRD hhhhhhhhhhhhhccccceeeeecccccccccccccceeeeccccccccccccccccccc MEM .... MMMMMMMMMMMMMMMMM SEQ DPGGPGMNPVGNSTAMAFQVPPNSPQGSVACPPPPAYCNTPPPPYEQVVKAK SEG xxxxxx xxxxxxxxxxxxxxxx PRD ccccccccccccccceeecccccccccccccccccccccccccccccccccc MEM (No Prosite data available for DKFZphfbr2_22k8.1) (No Pfam data available for DKFZphfbr2_22k8.1) Pedant information for DKFZphfbr2_22k8, frame 3 Report for DKFZphfbr2_22k8.3 [LENGTH] 122 [MW] 12854.08 [pi] 10.27 [KW] All_Alpha [KW] LOW_COMPLEXITY 25.41 % SEQ GSHEAPACEGGGAAARAALGVHRΞQKALLVFRRTLSNLLYMPLLRGLLWLQVLCAGPLHT SEG .... xxxxxxxxxxxxxxxx PRD ccccccccccchhhhhhhhccccchhhhhhhhhhhhhhhccccccchhhhhhhhcccccc SEQ EAVVLLVPSDDGRAFLLRSRLLHPEAHVPPAADRGASLQCVLHQAAPKSRPRSPAAGAAL SEG xxxxxxxxxxxxxxx . PRD cceeeeeccccchhhhhhhhccccccccccccccchhhhhhhhhccccccccchhhhhhc SEQ LH SEG PRD cc (No Prosite data available for DKFZphfbr2_22k8.3) (No Pfam data available for DKFZphfbr2_22k8.3) DKFZphfbr2_23blO group: nucleic acid managment DKFZphfbr2_2blO encodes a novel 580 amino acid protein with strong similarity to rat RNA helicase HEL117. HEL117 is a DEAD/H box helicase, which co-localises with a splicing factor and thus seems to be involved in splicing. The new protein can find application in modulation of splicing. strong similarity to rat RNA helicase HEL117 complete cDNA, complete eds, EST hits Sequenced by AGOWA Locus: unknown Insert length: 2905 bp Poly A stretch at pos. 2885, no polyadenylation signal found 1 GGGGGCTCCG CTCCGCACCA CCAACCCCGG GCCGCAGTCC TGACGAGCGG 51 GTCAGGGCTT GTCGGGCGGA AGCCTGGCCT GGAGCCTGGA AGGGGGAGAC 101 GGCCCGAGCG GGAGCGGGAG CGGACGCGGC CTCAGTCCTG CGCGGAATAT 151 TGAAGGATGT TTGTTCCAAG ATCTCTAAAA ATCAAGAGGA ATGCTAATGA 201 TGATGGCAAA AGTTGTGTGG CTAAGATAAT TAAACCAGAC CCAGAAGACC 251 TTCAGTTGGA CAAAAGCAGA GATGTTCCCG TTGATGCTGT AGCTACAGAA 301 GCAGCCACAA TAGACAGGCA CATCAGCGAA TCATGCCCTT TCCCCAGCCC 351 AGGTGGCCAG TTGGCAGAGG TTCATTCAGT AAGTCCCGAG CAGGGTGCGA 401 AGGACAGCCA TCCTTCTGAA GAGCCCGTTA AGTCATTTTC CAAAACACAG 451 CGCTGGGCAG AACCAGGGGA ACCCATCTGT GTTGTCTGTG GTCGTTATGG 501 AGAGTATATC TGTGATAAGA CAGATGAAGA TGTGTGTAGT TTGGAGTGTA 551 AAGCGAAACA TCTTCTACAA GTTAAGGAAA AGGAAGAGAA ATCAAAACTC 601 AGCAATCCAC AGAAGGCTGA TTCTGAGCCA GAGTCTCCAC TGAATGCTTC 651 CTATGTCTAC AAAGAGCACC CCTTTATTTT GAACCTTCAG GAAGACCAGA 701 TTGAAAATCT TAAACAGCAG CTGGGAATTT TAGTTCAAGG GCAAGAAGTC 751 ACCAGGCCCA TTATTGACTT TGAACATTGT AGTCTCCCTG AGGTCTTAAA 801 TCACAACTTG AAGAAATCAG GCTATGAGGT GCCAACTCCC ATTCAAATGC 851 AGATGATTCC TGTGGGACTT CTGGGAAGAG ACATTCTGGC CAGTGCAGAT 901 ACTGGCTCAG GAAAAACAGC TGCTTTTCTT CTTCCTGTTA TCATGCGAGC 951 TTTATTCGAG AGCAAAACTC CATCTGCGCT CATTCTTACA CCAACCAGAG 1001 AGTTAGCCAT TCAGATAGAG AGACAAGCTA AAGAATTGAT GAGTGGCCTG 1051 CCACGCATGA AAACTGTGCT TCTTGTAGGG GGCTTACCCT TACCCCCACA 1101 GCTTTATCGT CTGCAACAAC ATGTTAAGGT TATCATAGCA ACCCCTGGGC 1151 GACTTCTGGA TATAATAAAG CAGAGCTCTG TAGAACTCTG TGGTGTAAAG 1201 ATTGTGGTAG TAGATGAAGC TGATACCATG TTAAAGATGG GTTTTCAACA 1251 ACAAGTGCTT GACATTTTGG AAAACATTCC TAATGATTGT CAGACCATTT 1301 TGGTTTCAGC CACAATTCCA ACTAGCATAG AACAGCTAGC AAGCCAGCTT 1351 CTGCATAATC CTGTGAGAAT TATCACTGGA GAAAAGAACC TACCTTGTGC 1401 CAATGTACGT CAGATTATTT TGTGGGTAGA AGACCCAGCC AAAAAGAAAA 1451 AATTATTTGA AATTTTAAAT GATAAGAAAC TCTTTAAGCC TCCAGTGTTA 1501 GTATTTGTGG ACTGCAAACT AGGAGCAGAT CTTTTGAGTG AAGCCGTTCA 1551 GAAAATCACA GGGCTGAAAA GCATATCTAT ACATTCGGAG AAGTCGCAAA 1601 TAGAAAGGAA AAACATATTG AAGGGATTAC TTGAAGGAGA CTATGAAGTT 1651 GTAGTGAGCA CAGGAGTCTT GGGACGAGGC CTAGACTTGA TCAGTGTCAG 1701 GCTGGTTGTC AATTTTGATA TGCCTTCAAG TATGGATGAG TATGTCCATC 1751 AGGAAAATAC CTACAAGTCT ACTTGGAGGA ATCCCCAGCA TTTTCAACAG 1801 GATGTCAGAA TGACCTTGGG CTATGTTGGC AAAGCACAAT GGGAAGAAGA 1851 CAACCAATTG AAGGTCAAAC TAGGCCTTAA AAAAAATTGT TCTTCCTAAA 1901 TGAAACTTTA TGTAAGACCC AAGCTTCCTT TATGTAAAAA TAGGATACTC 1951 ACTAGGCTTT GGGGCTGACA ATGGTTTTTA AATCTTGCTA ATCTTCCCTG 2001 GAATGAAACC AGCATGACTC AAAGAGAAAA AGAGAGTCTA TAATATTTTC 2051 TAATCCCTGA GTTCTTTTCT TTATATATTA AAAAGGATTA TTAGGCTGGG 2101 TGTGGTGGCT CACGCCTGTA ATCCCAGCAC TTTGGGAGGC CGAGGGGAGT 2151 GGATCACCTG AGTTCGAGAC CAGCCTAACC AACATGGAGA AACCCTGTCT 2201 CTACTAAAAA TACAAAATTA GCCAGGCGTG GTGGCGCATG CCTGTAATCC 2251 CAGCTACTCA GGAGGCTACA GCAGGAGAAT TGCTTGAACT CGGGAGGCAG 2301 AGCCAAGATC GCACCACTGC ACTCCAGCCT GGGCAACAAG AGTGAAACTC 2351 TGTCTCAAAA TAATATTAAT GATAATAATA ATAATAATAA TAGGGATTAC 2401 TTGCATAATT GTTCTTTTAA AATTATTGGC AGTATTGCTG AATGTATTTA 2451 GATTTTTTCA CCAAGTGACA ACAACTGAAT TCATAAAGAT TCATCAACAA 2501 GACCTGATAA AAAAAAATGT AAGCATATTA TAGTGGATAC TTCCAAGACT 2551 CTTGGTCTAA CATGTATTAG AAAGCAGAAG GAGCCCAGGC ACAGGGGCTC 2601 CCGCCGGTAA TCCCAAAGCT TTGGGAAGCC AAGGCAGGTG GATCGCTTGA 2651 GCTCAGGAGT TAGAGACCAG CCTGGGCAAC ATGGTGAAAT CCCGTCACCA 2701 CAAAAAAATG CAAAAATTAA CTGGGCGTGG TGGCATGCAC CTGTAGTCCC 2751 AGCTACTCTG GAGGCTGAGG TGAGGGGAAT CACCTGAGCC GGGGGAATCA 2801 CCTGAGCCCA GGGAAGTTGA GGCTGCTGTG AGCCATGGTC ATGACACTGC 2851 CCTCCAGCCT GGACAACAGA TTGAGACCCT GTCTCAAAAA AAAAAAAAAA 2901 AAAAA BLAST Results No BLAST result Medlme entries Medline: A putative mammalian RNA helicase with an arginme-serine-rich domain Peptide information for frame 1 ORF from 157 bp to 1896 bp; peptide length: 580 Category: strong similarity to known protein Prosite motifs: ATP_GTP_A (247-255) LEUCINE ZIPPER (298-320) 1 MFVPRSLKIK RNANDDGKSC VAKIIKPDPE DLQLDKSRDV PVDAVATEAA 51 TIDRHISESC PFPSPGGQLA EVHSVSPEQG AKDSHPSEEP VKSFSKTQRW 101 AEPGEPICVV CGRYGEYICD KTDEDVCSLE CKAKHLLQVK EKEEKSKLSN 151 PQKADSEPES PLNASYVYKE HPFILNLQED QIENLKQQLG ILVQGQEVTR 201 PIIDFEHCSL PEVLNHNLKK SGYEVPTPIQ MQMIPVGLLG RDILASADTG 251 SGKTAAFLLP VIMRALFESK TPSALILTPT RELAIQIERQ AKELMSGLPR 301 MKTVLLVGGL PLPPQLYRLQ QHVKVIIATP GRLLDIIKQS SVELCGVKIV 351 VVDEADTMLK MGFQQQVLDI LENIPNDCQT ILVSATIPTΞ IEQLASQLLH 401 NPVRIITGEK NLPCANVRQI ILWVEDPAKK KKLFEILNDK KLFKPPVLVF 451 VDCKLGADLL SEAVQKITGL KSISIHSEKS QIERKNILKG LLEGDYEVVV 501 STGVLGRGLD LISVRLVVNF DMPSSMDEYV HQENTYKSTW RNPQHFQQDV 551 RMTLGYVGKA QWEEDNQLKV KLGLKKNCSS BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_23blO, frame 1 PIR:A57514 RNA helicase HEL117 - rat, N = 2, Score = 615, P = 1.6e-60 TREMBL:AB018344_1 gene: "KIAA0801"; product: "KIAA0801 protein"; Homo sapiens mRNA for KIAA0801 protein, complete eds., N = 1, Score = 615, P = 2.8e-59 TREMBL :CEF01F1_1 gene: "F01F1.7"; Caenorhabditis elegans cosmid F01F1., N = 2, Score = 365, P = 1.9e-58 TREMBL:AF083255_1 product: "RNA helicase-related protein"; Homo sapiens RNA helicase-related protein mRNA, complete eds., N = 2, Score = 556, P = 1.5e-57 PIR:S14048 RNA helicase dbp2 - fission yeast (Schizosaccharomyces pombe), N = 1, Score = 591, P = 1.6e-57 >PIR:A57514 RNA helicase HEL117 - rat Length = 1,032 HSPs: Score = 615 (92.3 bits), Expect = 1.6e-60, Sum P(2) = 1.6e-60 Identities = 140/394 (35%), Positives = 236/394 (59%) Query: 144 EKSKLSNPQKADSEPESPLNASYVYKEHPFILNLQEDQIENLKQQL-GILVQGQEVTRPI 202 ++ KL P P ++ Y E P + + ++++ + ++ GI V+G+ +PI Sbjct: 313 KQRKLLEPVDHGKIEYEPFRKNF-YVEVPELAKMSQEEVNVFRLEMEGITVKGKGCPKPI 371 Query: 203 IDFEHCSLPEVLNHNLKKSGYEVPTPIQMQMIPVGLLGRDILASADTGSGKTAAFLLPV- 261 + C + + ++LKK GYE PTPIQ Q IP + GRD++ A TGSGKT AFLLP+ Sbjct: 372 KSWVQCGISMKILNSLKKHGYEKPTPIQTQAIPAIMSGRDLIGIAKTGSGKTIAFLLPMF 431 Query: 262 --IM—RALFESKTPSALILTPTRELAIQIERQAKELMSGLPRMKTVLLVGGLPLPPQLY 317 IM R+L E + P A+I+TPTRELA+QI ++ K+ L ++ V + GG + Q+ Sbjct: 432 RHIMDQRSLEEGEGPIAVIMTPTRELALQITKECKKFSKTLG-LRVVCVYGGTGISEQIA 490 Query: 318 RLQQHVKVIIATPGRLLDIIKQSΞ VELCGVKIVVVDEADTMLKMGFQQQVLDILENI 374 L++ ++I+ TPGR++D++ +S L V VV+DEAD M MGF+ QV+ I++N+ Sbjct: 491 ELKRGAEIIVCTPGRMIDMLAANSGRVTNLRRVTYVVLDEADRMFDMGFEPQVMRIVDNV 550 Query: 375 PNDCQTILVSATIPTSIEQLASQLLHNPVRIITGEKNLPCANVRQIILWVEDPAKKKKLF 434 D QT++ SAT P ++E LA ++L P+ + G +++ C++V Q ++ +E+ K KL Sbjct: 551 RPDRQTVMFSATFPRAMEALARRILSKPIEVQVGGRSVVCSDVEQQVIVIEEEKKFLKLL 610 Query: 435 EILNDKKLFKPPVLVFVDCKLGADLLSEAVQKITGLKSISIHSEKSQIERKNILKGLLEG 494 E+L + V++FVD + AD L + + + + +S+H Q +R +1+ G Sbjct: 611 ELLGHYQE-SGSVIIFVDKQEHADGLLKDLMRAS-YPCMSLHGGIDQYDRDSIINDFKNG 668 Query: 495 DYEVVVSTGVLGRGLDLISVRLVVNFDMPSSMDEYVHQ 532 +++V+T V RGLD+ + LVVN+ P+ ++YVH+ Sbjct: 669 TCKLLVATSVAARGLDVKHLILVVNYSCPNHYEDYVHR 706 Score = 37 (5.6 bits), Expect = 1.6e-60, Sum P(2) = 1.6e-60 Identities = 13/36 (36%), Positives = 17/36 (47%) Query: 132 KAKHLLQVKEKEE KSKLSNPQKADSEPESPLNA 164 KA++ + KEK E SK K D E E +A Sbjct: 113 KAENRSRSKEKAEGGDSSKEKKKDKDDKEDEKEKDA 148 Pedant information for DKFZphfbr2_23blO, frame 1 Report for DKFZphfbr2_23blO .1 [LENGTH] 580 [MW] 64572.24 [pi] 6.13 [HOMOL] TREMBL :CEF01F1 1 gene: "F01F1.7"; Caenorhabditis elegans cosmid F01F1. 8e-61 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YNL112w] 2e-53 [FUNCAT] 04.01.04 rrna processing [S. cerevisiae, YNL112w] 2e-53 [FUNCAT] 04.05.03 mrna processing (splicing) [S. cerevisiae, YPL119c] 5e-53 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YOR204w] 2e-49 [FUNCAT] 05.04 translation (initiation, elongation and termination) [S. cerevisiae, YOR204W] 2e-49 [ FUNCAT ] j mrna translation and ribosome biogenesis [H. influenzae, HI0231 RNA] 2e-46 [FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YLL008w] 3e-43 [FUNCAT] 04.99 other transcription activities [Ξ. cerevisiae, YDL160c] 4e-39 [FUNCAT] 1 genome replication, transcription, recombination and repair [H. influenzae HI0892] 3e-35 [FUNCAT] 04.05.01.07 chromatin modification [S. cerevisiae, YMR290c] 6e-34 [FUNCAT] 98 classification not yet clear-cut [S. cerevisiae, YOR046c] 3e-32 [FUNCAT] 09.01 biogenesis of cell wall [S. cerevisiae, YJL033w] 8e-30 [FUNCAT] 30.16 mitochondrial organization [S. cerevisiae, YDR194C] 5e-23 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YGL064c] le-16 [FUNCAT] r general function prediction [M. jannaschii, MJ1401] 5e-ll [FUNCAT] 11.10 cell death [S. cerevisiae, YMR190c] le-06 [FUNCAT] 03.19 recombination and dna repair [S. cerevisiae, YMR190c] le-06 [BLOCKS] BL00115B Eukaryotic RNA polymerase II heptapeptide repeat proteins [BLOCKS] BL00039D DEAD-box subfamily ATP-dependent helicases proteins [BLOCKS] BL00039C DEAD-box subfamily ATP-dependent helicases proteins [BLOCKS] BL00039B DEAD-box subfamily ATP-dependent helicases proteins [BLOCKS] BL00039A DEAD-box subfamily ATP-dependent helicases proteins [PIRKW] nucleus 6e-53 [PIRKW] RNA binding 9e-52 [PIRKW] DEAD box 2e-43 [PIRKW] transmembrane protein le-21 [PIRKW] DNA binding 5e-48 [PIRKW] ATP 4e-57 [PIRKW] purine nucleotide binding 2e-43 [PIRKW] P-loop 4e-57 [PIRKW] hydrolase 6e-42 [PIRKW] protein biosynthesis 2e-43 [PIRKW] ATP binding 2e-50 [SUPFAM] WW repeat homology le-49 [SUPFAM] translation initiation factor eIF-4A 2e-43 [SUPFAM] DEAD/H box helicase homology 4e-57 [SUPFAM] recQ helicase homology 8e-06 [SUPFAM] unassigned DEAD/H box helicases 4e-57 [SUPFAM] ATP-dependent RNA helicase DBP1 2e-53 [SUPFAM] ATP-dependent RNA helicase DHH1 6e-40 [SUPFAM] tobacco ATP-dependent RNA helicase DB10 le-49 [SUPFAM] Bloom's syndrome helicase 8e-06 [PROSITE] ATP_GTP_A 1 [PROSITE] LEUCINE_ZI PPER 1 [PROSITE] MYRISTYL 6 [PROSITE] CK2_PHOSPHO_SITE 8 [PROSITE] TYR_PHOSPHO_SITE 1 [PROSITE] PKC_PHOSPHO_SI E 7 [PROSITE] ASN_GLYCOSYLATION 1 [PFAM] Helicases conserved C-termmal domain [PFAM] DEAD and DEAH box helicases [KW] Alpha_Beta [KW] LOW COMPLEXITY 3.10 % SEQ MFVPRΞLKIKRNANDDGKSCVAKIIKPDPEDLQLDKSRDVPVDAVATEAATIDRHISEΞC SEG PRD ccccceeeeccccccccceeeeeeeeccccceeecccccccccchhhhhhhhhhhhcccc SEQ PFPSPGGQLAEVHSVSPEQGAKDSHPSEEPVKSFSKTQRWAEPGEPICVVCGRYGEYICD SEG PRD cccccccceeeeccccccccccccccccccccccccccccccccccceeeeccccceeec SEQ KTDEDVCSLECKAKHLLQVKEKEEKSKLSNPQKADΞEPESPLNASYVYKEHPFILNLQED SEG PRD cccccccchhhhhhhhhhhhhhccccccccccccccccccccccceeeccccccccchhh SEQ QIENLKQQLGILVQGQEVTRPIIDFEHCSLPEVLNHNLKKSGYEVPTPIQMQMIPVGLLG SEG PRD hhhhhhhhheeeeccccccccccccccccchhhhhhhhhhhccccccccccccceeeecc SEQ RDILASADTGΞGKTAAFLLPVIMRALFESKTPSALILTPTRELAIQIERQAKELMSGLPR SEG PRD cceeeeeccccccceeeehhhhhhhhcccccceeeeecchhhhhhhhhhhhhhhhccccc SEQ MKTVLLVGGLPLPPQLYRLQQHVKVIIATPGRLLDIIKQSSVELCGVKIVVVDEADTMLK SEG ... xxxxxxxxxxxxxxxxx PRD eeeeeeecccccchhhhhhhhheeeeeeccccchhhhhhheeeeeeeeeeeehhhhhhhh SEQ MGFQQQVLDILENIPNDCQTILVSATIPTSIEQLASQLLHNPVRIITGEKNLPCANVRQI SEG PRD cccchhhhhhhhhcccccceeeeecccchhhhhhhhhhhhceeeeeeeccccccccccce SEQ ILWVEDPAKKKKLFEILNDKKLFKPPVLVFVDCKLGADLLSEAVQKITGLKSISIHSEKS SEG PRD eeecccchhhhhhhhhhhhhccccceeeeeeecccchhhhhhhhhhhhccceeeccccch SEQ QIERKNILKGLLEGDYEVVVSTGVLGRGLDLISVRLVVNFDMPSSMDEYVHQENTYKSTW SEG PRD hhhhhhhhhhhccccceeeeehhhhhhcccceeeeeeeeecccccccceeeecccccccc SEQ RNPQHFQQDVRMTLGYVGKAQWEEDNQLKVKLGLKKNCSS SEG PRD ccccccchhhhhhhccccchhhhhhhhhhhhhhhcccccc Prosite for DKFZphfbr2 23bl0.1 PS00001 163->167 ASN_GLYCOSYLATION PDOC00001 PS00005 6->9 PKC_PHOΞPHO_SITE PDOC00005 PS00005 97->100 PKC_PHOSPHO_SITE PDOC00005 PS00005 251->254 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 477->480 PKC_PHOSPHO_SITE PDOC00005 PS00005 513->516 PKC_PHOSPHO_SITE PDOC00005 PS00005 535->538 PKC_PHOSPHO_SITE PDOC00005 PS00005 539->542 PKC_PHOSPHO_SITE PDOC00005 PS00006 122->126 CK2_PHOSPHO_SITE PDOC00006 PS00006 156->160 CK2_PHOSPHO_SITE PDOC00006 PS00006 209->213 CK2_PHOSPHO_SITE PDOC00006 PS00006 221->225 CK2_PHOSPHO_SITE PDOC00006 PS00006 340->344 CK2_PHOSPHO_SITE PDOC00006 PS00006 389->393 CK2_PHOSPHO_SITE PDOC00006 PS00006 480->484 CK2_PHOSPHO_SITE PDOC00006 PS00006 524->528 CK2_PHOSPHO_SITE PDOC00006 PS00007 489->497 TYR_PHOSPHO_SITE PDOC00007 PS00008 66->72 MYRISTYL PDOC00008 PS00008 80->86 MYRISTYL PDOC00008 PS00008 195->201 MYRISTYL PDOC00008 PS00008 250->256 MYRISTYL PDOC00008 PS00008 490->496 MYRISTYL PDOC00008 PS00008 573->579 MYRISTYL PDOC00008 PS00017 247->255 ATP_GTP_A PDOC00017 PS00029 298->320 LEUCINE ZIPPER PDOC00029 Pfam for DKFZphfbr2_23bl0.1 HMM_NAME DEAD and DEAH box helicases HMM *gLpPWILRnIyeMGFEkPTPIQQqAIPHLeGRDVMACAQTGSGKTAAF +LP+ + N+++ G+E PTPIQ+Q IP+ L GRD++A A TGSGKTAAF Query 209 SLPEVLNHNLKKSGYEVPTPIQMQMIPVGLLGRDILASADTGSGKTAAF 257 HMM HPMLQHIDwdPWpqpPQdPrALILAPTRELAMQIQEEcRkFgkHMnglR L+P++ + + + ++P ALIL+PTRELA+QI+++++++ + ++ ++ Query 258 LLPVIMRALFES—KTPS ALILTPTRELAIQIERQAKELMSGLPRMK 302 HMM ImcIYGGtnMRdQMRmLeRGpPHIVIATPGRLIDHIERgtldLDrleMLV ++++GG+++ +Q+ +L++ + ++IATPGRL+D+I++ ++ L ++++V Query 303 TVLLVGGLPLPPQLYRLQQHV-KVIIATPGRLLDIIKQSSVELCGVKIVV 351 HMM MDEADRMLDMGFIDQIRrIMrqlPMpwNRQTMMFSATMPdeIqELARrFM DEAD ML MGF++Q+ +1+ IP + QT++ SAT+P +I++LA ++ Query 352 VDEADTMLKMGFQQQVLDILENIP—NDCQTILVSATIPTSIEQLASQLL 399 HMM RNPIRInldMdElTtnEnlkQwYiyVerEMWKfdcLcrLIe* +NP+RI+ ++++L N++Q++ +VE + K +L+++++ Query 400 HNPVRIITGEKNLPCA-NVRQIILWVE-DPAKKKKLFEILN 438 HMM_NAME Helicases conserved C-terminal domain HMM *EιleeWLknl . GIrvmYIHGdMpQeERdelMddFNnGEynVLIcTDVgg ++L+E ++ G++ ++IH+ ++Q ER +I++ +G+Y V ++T V+G Query 458 DLLSEAVQKITGLKSISIHSEKSQIERKNILKGLLEGDYEVVVSTGVLG 506 HMM RGIDIPdVNHVINYDMPWNPEqYIQRIGRTgRIG* RG+D+++V++V+N+DMP +++ Y++ + T + Query 507 RGLDLISVRLVVNFDMPSSMDEYVH-QENTYKST 539 DKFZphfbr2_23b21 group: signal transduction DKFZphfbr2_23b21.1 encodes a novel 193 amino acid protein which is nearly identical to bovme neurocalcin. Neurocalcin is a Ca (2+) -binding protein with three putative Ca (2+) -binding domains (EF-hands). In cattle, 6 isoforms are differentially expressed in the central nervous system, retina and adrenal gland. Homology with recoverin indicates involvement in Ca2+ dependent activation of guanylate cyclase. The new protein can find application in modulating/blocking the guanylate cyclase-pathway . nearly identical to bovme neurocalcin eds complete cDNA Sequenced by AGOWA Locus: /map="574.6 cR from top of Chr8 linkage group" Insert length: 3300 bp Poly A stretch at pos. 3279, polyadenylation signal at pos. 3249 1 GGGGAGAATC TGGTGGATGC TGGACCTTGC TGCTGCTGCT ACTGCTGTTT 51 CCAGGGGCTG CAGAGCATGG ACTGTTAAAT CTTGCACTTC TTCTGAGTGA 101 GCTGAATTCT TGCCGCCAGG ATGGGGAAAC AGAACAGCAA GCTGCGCCCG 151 GAGGTCATGC AGGACTTGCT GGAAAGCACA GACTTTACAG AGCATGAGAT 201 CCAGGAATGG TATAAAGGCT TCTTGAGAGA CTGCCCCAGT GGACATTTGT 251 CAATGGAAGA GTTTAAGAAA ATATATGGGA ACTTTTTCCC TTATGGGGAT 301 GCTTCCAAAT TTGCAGAGCA TGTCTTCCGC ACCTTCGATG CAAATGGAGA 351 TGGGACAATA GACTTTAGAG AATTCATCAT CGCCTTGAGT GTAACTTCGA 401 GGGGGAAGCT GGAGCAGAAG CTGAAATGGG CCTTCAGCAT GTACGACCTG 451 GACGGAAATG GCTATATCAG CAAGGCAGAG ATGCTAGTGA TCGTGCAGGC 501 AATCTATAAG ATGGTTTCCT CTGTAATGAA AATGCCTGAA GATGAGTCAA 551 CCCCAGAGAA AAGAACAGAA AAGATCTTCC GCCAGATGGA CACCAATAGA 601 GACGGAAAAC TCTCCCTGGA AGAGTTCATC CGAGGAGCCA AAAGCGACCC 651 GTCCATTGTG CGCCTCCTGC AGTGCGACCC GAGCAGTGCC GGCCAGTTCT 701 GAGCCCTGCG CCCACCAATC GAATTGTAGA GCTGCTTGTG TTCCCTTTTG 751 ATTCTTCTTT TTAACAATTT TTTTTTTTTT TTGCCAAACA ATATCAATGG 801 TGATGCCGTC CCCTGTGCGG TCTGATGCGC CTTCCTCCGT GACGCCTTCA 851 GCCTCTTTTG TCGTGGATGC TTCGTGGGAA TGCCCAGAGC CCCAGTGTGC 901 TTGTGGAGAG CATGGACAGA CTTCGTGGTG TTCATTGTTT GATGATTTTT 951 AATCGTTACT ATTATTTCTT TTTATTCTAA TGTCTCTGTT CTAAAACGTA 1001 AGACTCGGGG GTTGGGGCAA AAGAAGGGAA ACCCATCCAG TCCTGTGATT 1051 CTATTGCAAG CTTCAAGGGG CTTTTGTTTG AAAGACAAAA CTCCCCACCT 1101 GGGTCTGTTG TCACACGTGC CGTAGGGGTG ATGGATGGCA CCGGATGCTG 1151 GATTCCCCAA GAACAAGTTA CCCTCTGGGG TGAGGCTATT CCAGCGAGCT 1201 GGGACATTTC CCCATGGGGG CCCACTCCCC TCTCTTCCCC AGCAGGCTGT 1251 AGTTTCTAAG CTGTGAACAT TTCAAGATAA ATTAACAGAG GAGAGGAAAA 1301 AGATGGCTCA GCTATTTTTT CACAGGTTTA CACTAGTTGA GCTAATATGC 1351 GTGTCTTTGG AAATTAAACA CAAATGGTAA CATATTCCAA AACCAGACCC 1401 ATCTTGTTGC CTATTGTGAT AAAATAAAAA GACGGCTGTA TATAACATAT 1451 TGGGTAATGC AGACCAAATT AAGTGTTTTG CCTTGTTTAA ATGAAATGCA 1501 TGTTTAGTGA GCACTAATAC AATCTTATTC CAGAAGACTG TTTTTAGTAG 1551 CTTATTGTGA AGTAAGACAA CTATAATGAA TGTCTGTCTT GTTTGGAAGT 1601 CATATCTGTC TTTGCACAAA TGTACCAATC GACAAGTATA TTTTATATAT 1651 TCCATAAAAA TACAAAGTAA CCCTGACTAG GGCCCAACTT TAATTTTGAA 1701 TGCATTTCCA GAGTGGCCAT GCCTAGAGGG CAGATGCAGA GCAGGTGGTA 1751 GTGGGACAGG ACAATTGGAG CACAGGAATG TTAACATGTA TGACAGGGGA 1801 CCAGTAGGGT GGTTTCCCTC TCAGGCCCAG CAGCCCATTG ACAGCATTAG 1851 ACTGGCGGCA TGGTGCTTTT CTGAGCAGAT CAATACTCTG CAGACTCGAA 1901 AAAACATCAC ATACATTCTT GGAACTTCCC AGTGGTTTAA TCTATGTGCA 1951 TGGTTAGGGA GCCAGGCCTG GAATATTCAG TTTCCCTGCC CCTGTTAAAG 2001 AATCAGAGGT TGGGCAGTCA TCAAATTCAT CATAAAGACA TGGGCAAGTG 2051 TGTCTGTGGT TTCCAAGGCC CCCCTATGGA GAATCCAAAA GTATTTTCCA 2101 TTGCCGTGCT CTTTGAATGC AGACTTCTAT TTCCAGAAGT GACAGCACAA 2151 GTCTGAGTTG CTGTTTGGTC TGGTGACCTC AGACACACTA ATTTGAATTG 2201 AAAGCTAAGA GTAAAAATTT GCTGGTTACA GGCGAGTCAT ACTCTTGCAA 2251 GTAGTTAGCA AAGGGAGGCC CAAATTCTCA AGGTTGTTGA TGGGGAACTT 2301 GCCACTAAGA GAAGGCAGAG AGGTCCCTAG TGGGTATATT TGCTGCCAAG 2351 CCACTTGCCA AAGAAGAGGA ACCACAGAAA GAGAGACATC ATGACCAGGA 2401 GAAAAATGTG ACTAGACATG CTAACCTCCA GGTTTTTATA TATGACTTGA 2451 GTCTGCTGTA ATTGGCAGCA GAAATCCAAA TTTGTATGGT AGACCAAAAA 2501 GAACCAAATC CATAGGGTGA AATTTTGAGA CCTAGACTCT GTAAAAATAA 2551 TCCTAGTCTT CCTCCAGGGG TCAGTTCCTC ACAGTGGTTC TGTACCAAAA 2601 CTTGCCAAAT TCCTCCATGG CCAAGTGTTA AAATCTGTGT TTGGAAAATA 2651 GCGAATTAAC CTAAGACACA GAAGGCAGAC TGGGTGAGGA GACCTAGCAT 2701 GCCCTATTGG CAGTGCTCAG GAGCTGCATC CCACTTTTCC CTGCTCTGAA 2751 TCGAAGTCCT AGTTCCTTCC TTTGATTCTC CTTTGGTAGG TGGAATCAGT 2801 TAATGTTTTG AGAAACCTGC CTGGGCTCTG CCCTTAGTCA TGACATCTCG 2851 CTGAGCCAGA CCCACTCTGT TCCTTGGAAC CTAGAGCTGG AGTGAGGAGT 2901 AGAGGTCTCC GGCTATTCCA GAAAGAAAAG TGAGCCACAT GCAGGCTGAT 2951 GAATGCCGAC ACTTCCAGAA TGTATAGAAA TAGTCCCTGT CCTGGCCTGC 3001 CACTGACCCT GTCTGTATTT TCTCGGAGGT TGTTTTTCTC CTTCTCCTTC 3051 CCAGGAAGGT CTTTGTATGT CGAATCCAGT GCACTCAAGT TTGGCCAAGG 3101 GACTCCACAG CACCCAGAGG ACTGCATGCC TCAAGGTTTA TGTCACTCCT 3151 CTGCTGGGCT GTTCATTGTC ATTGCTGTGT TCAGGGACCT TTGGAAATAA 3201 AACCTGTTCT GTCCCAAATA AAACCAGCCT GTGATGTTCA AGGGACTGGA 3251 ATAAAGTGGC TTACGACCTG AAGGATTCTA AAAAAAAAAA AAAAAAAAAA BLAST Results Entry HS431350 from database EMBL: human STS I-15914. Score = 1308, P = 3.1e-53, identities = 276/285 Entry HSG19929 from database EMBL: human STS A002C26. Score = 926, P = 1.5e-35, identities = 186/187 Entry AF052142 from database EMBL: Homo sapiens clone 24665 mRNA sequence. Score = 7378, P = O.Oe+00, identities = 1482/1487 3' UTR Medlme entries 93247712: Neurocalcin family: a novel calcium-bindmg protein abundant in bovine central nervous system. 94045365: Distinct regional localization of neurocalcin, a Ca (2+) -binding protein, m the bovine adrenal gland. 96407688: Crystallization and preliminary X-ray crystallographic studies of recombinant bovine neurocalcin delta. 96066284: Distribution pattern of three neural calcium-binding proteins (NCS-1, VILIP and recoverin) in chicken, bovine and rat retina. Peptide information for frame 1 ORF from 121 bp to 699 bp; peptide length: 193 Category: strong similarity to known protein Prosite motifs: EF_HAND (73-86) EF_HAND (109-122) EF HAND (157-170) 1 MGKQNSKLRP EVMQDLLEST DFTEHEIQEW YKGFLRDCPS GHLSMEEFKK 51 IYGNFFPYGD ASKFAEHVFR TFDANGDGTI DFREFIIALS VTSRGKLEQK 101 LKWAFSMYDL DGNGYISKAE MLVIVQAIYK MVSSVMKMPE DESTPEKRTE 151 KIFRQMDTNR DGKLSLEEFI RGAKSDPSIV RLLQCDPSSA GQF BLASTP hits Entry JH0616 from database PIR: neurocalcin (clone pCalN) - bovine Score = 1001, P = 5.2e-101, identities = 192/193, positives = 192/193 Entry GGU91630_1 from database TREMBL: product: "neurocalcin"; Gallus gallus neurocalcin mRNA, complete eds. Score = 998, P = l.le-100, identities = 191/193, positives = 192/193 Entry NECD_BOVIN from database SWIΞSPROT: NEUROCALCIN DELTA. Score = 996, P = 1.8e-100, identities = 191/192, positives = 191/192 Entry S47565 from database PIR: BDR-1 protein - human Score = 934, P = 6.6e-94, identities = 174/193, positives = 187/193 Entry 150676 from database PIR: gene Rem-1 protein - chicken >TREMBL:GGREM1_1 gene: "Rem-1"; G. gallus rem-1 mRNA Score = 933, P = 8.4e-94, identities = 174/193, positives = 186/193 Alert BLASTP hits for DKFZphfbr2_23b21, frame 1 No Alert BLASTP hits found Pedant information for DKFZphfbr2_23b21, frame 1 Report for DKFZphfbr2_23b21.1 [LENGTH] 193 [MW] 22215.30 [pi] 5.35 [HOMOL] PIR:JH0616 neurocalcin (clone pCalN) - bovme le-109 [FUNCAT] 98 classification not yet clear-cut [S. cerevisiae, YDR373w] 3e-54 [FUNCAT] 30.03 organization of cytoplasm [Ξ. cerevisiae, YKL190w] 2e-18 [FUNCAT] 03.07 pheromone response, mating-type determination, sex-specific proteins [S. cerevisiae, YKL190w] 2e-18 [FUNCAT] 03.01 cell growth [S. cerevisiae YKL190w] 2e-18 [FUNCAT] 13.04 homeostasis of other ions [S. cerevisiae, YKL190w] 2e-18 [FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YKL190w] 2e-18 [FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YBR109C] 0.001 [FUNCAT] 08.19 cellular import [S. cerevisiae, YBR109c] 0.001 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YBR109C] 0.001 [FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YBR109c] 0.001 [ FUNCAT] 10.02.99 other morphogenetic activities [S. cerevisiae, YBR109c] 0.001 [FUNCAT] 30.05 organization of centrosome [S. cerevisiae, YBR109c] 0.001 [BLOCKS] BL00018 [SCOP] dlrec 1.34 .1.5.18 Recoverin [bovine (Bos taurus) 8e-55 [SCOP] dl sa 1.34.1.5.17 Recoverin [human (Homo sapiens) 5e-58 [SCOP] dltcob_ 1.34.1.5.16 Calcineurin regulatory subunit (B-chain le-06 [SCOP] d2mysc_ 1.34.1.5.15 Myosin Regulatory Chain [chicken (Gallu 2e-29 [SCOP] dlscmc 1.34.1.5.14 Myosin Regulatory Chain [bay scallo 5e-33 [SCOP] d2mysb _ 1.._34..1.5.13 Myosin Essential Chain [chicken (Gallu 4e-26 [SCOP] dlscmb 1.34.1.5.12 Myosin Essential Chain [bay scallo 6e-27 [SCOP] dlclm_ 1.34.1.5.11 Calmodulin [Paramecium tetraurelia le-15 [SCOP] d4cln 1.34.1 5.10 Calmodulin [Drosophila melanogaster 2e-16 [SCOP] dlcfc 1.34.1.5.9 Calmodulin [African frog (Xenopus laevis) 2e-16 [SCOP] dlahr ' 11.34.1.5.8 Calmodulin [chicken gallus gallus 4e-16 [SCOP] d3cln 34.1.5 Calmodulin [rat (Rattus rattus) 2e-16 [SCOP] dltrcb_ 34.1.5 6 Calmodulin [bovme (Bos taurus) 8e-08 [SCOP] dlcll 34.1.5.5 Calmodulin [human (Homo sapiens) 2e-16 [SCOP] dlrtpl_ 34.1.4.5 Parvalbumin [rat (Rattus rattus) 8e-06 [SCOP] d5tnc 34.1.5.2 Troponin C [turkey (Meleagris gallopavo) 3e-13 [SCOP] dlpvaa_ 34.1...4,.3 Parvalbumin [pike (Esox lucius) 6e-06 [SCOP] dltnp x . 34.1.5.1 Troponin C [chicken (Gallus gallus) 9e-ll [EC] 2.7.1.107 Diacylglycerol kinase 2e-08 [PIRKW] blocked amino end le-100 [PIRKW] phosphotransferase 2e-08 [PIRKW] duplication 4e-17 [PIRKW] tandem repeat 7e-06 [PIRKW] heterodimer 4e-17 [PIRKW] heart 6e-09 [PIRKW] zinc 2e-08 [PIRKW] serine/threonine-specific protein kinase le-06 [PIRKW] muscle contraction le-08 [PIRKW] acetylated amino end 4e-09 [PIRKW] ATP 2e-08 [PIRKW] skeletal muscle 6e-09 [PIRKW] signal transduction le-91 [PIRKW] protein kinase 2e-08 [PIRKW] calcium binding le-100 [PIRKW] alternative splicing 2e-13 [PIRKW] methylated amino acid le-09 [PIRKW] thin filaments le-08 [PIRKW] lipoprotein le-101 [PIRKW] cardiac muscle 6e-09 [PIRKW] muscle 6e-09 [PIRKW] myristylation le-100 [PIRKW] EF hand le-101 [PIRKW] retina 2e-51 [SUPFAM] calcium-dependent protein kinase 2e-08 [SUPFAM] unassigned calmodulin-related proteins 8e-41 [SUPFAM] spec-related protein LpSl 7e-06 [SUPFAM] calmodulin repeat homology le-101 [SUPFAM] human diacylglycerol kinase 2e-08 [SUPFAM] protein kinase C zinc-binding repeat homology 2e-0£ [SUPFAM] protein kinase homology 2e-08 [SUPFAM] calmodulin le-101 [PROSITE] EF_HAND 3 [PROSITE] CK2_PHOSPHO_SITE 7 [PROSITE] PKC_PHOSPHO_SITE 3 [PFAM] EF hand [KW] All_Alpha [KW] 3D SEQ MGKQNSKLRPEVMQDLLESTDFTEHEIQEWYKGFLRDCPSGHLSMEEFKKIYGNFFPYGD lrec- HHHHHHHHHTTTTCCCHHHHHHHHHHHHHHTTTTEEEHHHHHHHHHHHTTTTC SEQ ASKFAEHVFRTFDANGDGTIDFREFIIALSVTSRGKLEQKLKWAFSMYDLDGNGYISKAE lrec- HHHHHHHHHHHH CEEEHHHHHHHHHHHHCCCGGGHHHHHHHHHTTTTCCCEEHHH SEQ MLVIVQAIYKMVSSVMKMPEDESTPEKRTEKIFRQMDTNRDGKLSLEEFIRGAKSDPSIV lrec- HHHHHHHHHHCCTTGGGCTTTTTCHHHHHHHHHHHHCCTTTTEECHHHHHHHHHHCHHHH SEQ RLLQCDPSSAGQF lrec- HHHCCCH Prosite for DKFZphfbr2_23b21.1 PS00005 92->95 PKC PHOSPHO SITE PDOC00005 PS00005 149->152 PKC PHOSPHO " " SITE PDOC00005 PS00005 158->161 PKC PHOSPHO " " SITE PDOC00005 PS00006 23->27 CK2 PHOSPHO " " SITE PDOC00006 PS00006 44->48 CK2 PHOSPHO " " SITE PDOC00006 PS00006 106->110 CK2 PHOSPHO " ' SITE PDOC00006 PS00006 117->121 CK2 PHOSPHO " " SITE PDOC00006 PS00006 143->147 CK2 PHOSPHO " " SITE PDOC00006 PS00006 158->162 CK2 PHOSPHO- " SITE PDOC00006 PS00006 165->169 CK2 PHOSPHO- " SITE PDOC00006 PS00018 73->86 EF HAND PDOC00018 PS00018 109->122 EF HAND PDOC00018 PS00018 157->170 EF HAND PDOC00018 Pfam for DKFZphfbr2_23b21.1 HMM_NAME EF hand HMM *MFrmMDkDGDGyIDFEEFmeMMkem* +FR +D +GDG+IDF EF+ +++ Query 68 VFRTFDANGDGTIDFREFIIALSVT 92 30.75 100 128 1 29 dkfzphfbr2_23b21.1 nearly identical to bovine neurocalcin Alignment to HMM consensus: Query *EIqEMFrmMDkDGDGyIDFEEFmeMMkem* ++++F+M+D DG+GYI++ E++++++++ dkfzphfbr2 100 KLKWAFSMYDLDGNGYISKAEMLVIVQAI 128 Query 176 1 29 dkfzphfbr2_23b21.1 nearly identical to bovine neurocalcin Alignment to HMM consensus: HMM *EIqEMFrmMDkDGDGyIDFEEFmeMMkem* +++FR MD+++DG+++ EEF++ K+ Query 148 RTEKIFRQMDTNRDGKLSLEEFIRGAKSD 176 DKFZphfbr2_23f2 group: brain derived DKFZphfbr2_23f2 encodes a novel 182 amino acid protein with weak similarity to S. pombe Vps29p. No informative BLAST results; no predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of brain-specific genes . similarity to Vps29p complete cDNA, complete eds, EST hits S. cerevisiae and S. pombe Vps29p are involved in vacuolar protein sorting part of the cDNA is encoded by HSAC2350, splice pattern 4 exons Sequenced by AGOWA Locus: /map="12q24" Insert length: 1016 bp Poly A stretch at pos. 996, polyadenylation signal at pos. 974 1 GAATGGGGAG GAGCCAGAGG AAGAGGGCGG CGACGGTGGT GGTGACTGAG 51 CGGAGCCCGG TGACAGGATG TTGGTGTTGG TATTAGGAGA TCTGCACATC 101 CCACACCGGT GCAACAGTTT GCCAGCTAAA TTCAAAAAAC TCCTGGTGCC 151 AGGAAAAATT CAGCACATTC TCTGCACAGG AAACCTTTGC ACCAAAGAGA 201 GTTATGACTA CCTCAAGACT CTGGCTGGTG ATGTTCATAT TGTGAGAGGA 251 GACTTCGATG AGAATCTGAA TTATCCAGAA CAGAAAGTTG TGACTGTTGG 301 ACAGTTCAAA ATTGGTCTGA TCCATGGACA TCAAGTTATT CCATGGGGAG 351 ATATGGCCAG CTTAGCCCTG TTGCAGAGGC AATTTGATGT GGACATTCTT 401 ATCTCGGGAC ACACACACAA ATCTGAAGCA TTTGAGCATG AAAATAAATT 451 CTACATTAAT CCAGGTTCTG CCACTGGGGC ATATAATGCC TTGGAAACAA 501 ACATTATTCC ATCATTTGTG TTGATGGATA TCCAGGCTTC TACAGTGGTC 551 ACCTATGTGT ATCAGCTAAT TGGAGATGAT GTGAAAGTAG AACGAATCGA 601 ATACAAAAAA CCTTAAAGCC AGGCCTGTCT TGATGATTTT TGGTTTTTTT 651 TCATTGTCCT GTTGAAATCA AGTAATTAAA CATTTAAGAG CCACAAAATT 701 GTATCACTTT TATAATATTT TGCAGTAAAA TATAATACCA TCTTCTCTGT 751 TAATACATAA TTGCTCCAAG CTTCCTGTAA ACTATAAGAA TATATTTAGT 801 TTACAGTATA TGGATTCTAT GAAAAAATGT CCACAACACA GTAATTGGTC 851 ACTTGTTAAG AAAAATTTAT CCTTGTAAGT ATCTTCAAAG TTGATATTTG 901 GAACTTTATT CCAAAAGTAG TGCATGTGGA GAAAGAATCT AGACTTTCTT 951 GTATACATTT TTCTCTTCTC CAGTAATAAA CAATTACCTT TCATTGAAAA 1001 AAAAAAAAAA AAAAAA BLAST Results Entry HSAC2350 from database EMBLNEW: Homo sapiens 12q24 PAC P424M6 Length = 167,217 Medlme entries No Medline entry Peptide information for frame 2 ORF from 68 bp to 613 bp; peptide length: 182 Category: similarity to known protein Prosite motifs: RGD (60-63) 1 MLVLVLGDLH IPHRCNSLPA KFKKLLVPGK IQHILCTGNL CTKESYDYLK 51 TLAGDVHIVR GDFDENLNYP EQKVVTVGQF KIGLIHGHQV IPWGDMASLA 101 LLQRQFDVDI LISGHTHKSE AFEHENKFYI NPGSATGAYN ALETNIIPSF 151 VLMDIQASTV VTYVYQLIGD DVKVERIEYK KP BLASTP hits Entry CEZK1128_6 from database TREMBL: "ZK1128.1"; Caenorhabditis elegans cosmid ZK1128 Length = 523 Score = 400 (140.8 bits), Expect = 2.3e-37, P = 2.3e-37 Identities = 81/150 (54%), Positives = 106/150 (70%) Entry S46793 from database PIR: hypothetical protein YHR012c - yeast (Saccharomyces cerevisiae) Length = 282 Score = 180 (63.4 bits), Expect = 3.7e-37, Sum P(3) = 3.7e-37 Identities = 35/71 (49%), Positives = 44/71 (61%) Entry AB011824_1 from database TREMBL: "Vps29"; Schizosaccharomyces pombe mRNA for Vps29, partial eds. Schizosaccharomyces pombe (fission yeast) Length = 176 Score = 189 (66.5 bits), Expect = 2.7e-27, Sum P(2) = 2.7e-27 Identities = 33/72 (45%), Positives = 50/72 (69%) Alert BLASTP hits for DKFZphfbr2_23f2, frame 2 No Alert BLASTP hits found Pedant information for DKFZphfbr2_23f2, frame 2 Report for DKFZphfbr2_23f2.2 [LENGTH] 182 [MW] 20445.84 [pi] 6.29 [HOMOL] TREMBL:CEZK1128_6 gene: "ZK1128.8"; Caenorhabditis elegans cosmid ZK1128 2e-51 [FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YHR012w] le-27 [FUNCAT] 08.13 vacuolar transport [S. cerevisiae, YHR012w] le-27 [FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YHR012w] le-27 [FUNCAT] 30.08 organization of golgi [S. cerevisiae, YHR012w] le-27 [FUNCAT] 09.25 vacuolar and lysosomal biogenesis [S. cerevisiae, YHR012w] le-27 [FUNCAT] r general function prediction [M. jannaschn, MJ0623] le-16 [BLOCKS] BL01269D [BLOCKS] BL01269A [PROSITE] RGD 1 [PROSITE] MYRISTYL 4 [PROSITE] PKC_PHOSPHO_SITE 1 [KW] Alpha_Beta SEQ MLVLVLGDLHIPHRCNSLPAKFKKLLVPGKIQHILCTGNLCTKEΞYDYLKTLAGDVHIVR PRD ccceeecccccccccccchhhhhhhhhhcceeeeeecccccchhhhhhhhhhhhceeeee SEQ GDFDENLNYPEQKVVTVGQFKIGLIHGHQVIPWGDMASLALLQRQFDVDILISGHTHKSE PRD cccccccccccceeeeeccceeeeecccccccccchhhhhhhhhhhcceeeeeccccccc SEQ AFEHENKFYINPGSATGAYNALETNIIPSFVLMDIQASTVVTYVYQLIGDDVKVERIEYK PRD ccccccccccccccccccccccccccccceeeeeccccceeeeeeeecccceeeeeeeec SEQ KP PRD cc Prosite for DKFZphfbr2_23f2.2 PS00005 116->119 PKC_PHOSPHO_ΞITE PDOC00005 PS00008 38->44 MYRISTYL PDOC00008 PS00008 83->89 MYRISTYL PDOC00008 PS00008 133->139 MYRISTYL PDOC00008 PS00008 137->143 MYRISTYL PDOC00008 PS00016 60->63 RGD PDOC00016 (No Pfam data available for DKFZphfbr2_23f2.2) DKFZphfbr2_23124 group: intracellular transport and trafficking DKFZphfbr2_23124.2 encodes a novel 348 amino acid protein with similarity to human glycoprotein gp36b and canine VIP36 glycoprotein. The vesicular protein VIP36 (36 kDa vesicular integral membrane protein) shows homology to leguminous plant lectins . The protein is localized to the Golgi apparatus, endosomal and vesicular structures and the plasma membrane. VIP36 binds to sugar residues of glycosphmgolipids and/or glycosylphosphatidyl-inositol anchors and might provide a l nk between the extracellular/lummal face of glycolipid rafts and the cytoplasmic protein segregation machinery. Gp36 is located within the endoplasmatic reticulum. For the novel protein, a lectin character is predicted. Due to the intracellular localisation of the homolog proteins, it should be involved in intracellular transport and trafficking. The new protein can find application in modulating/blocking intracellular transport and trafficking. strong similarity to human GP36b glycoprotein complete cDNA, complete eds, EST hits potential start at Bp 29 matches kozak consensua ANNatgG similarity to lectins, Sequenced by AGOWA Locus: /map="2" Insert length: 2416 bp Poly A stretch at pos. 2394, no polyadenylation signal found 1 GGGGGATGAA GGGTCGTTGG TGGGAAAGAT GGCGGCGACT CTGGGACCCC 51 TTGGGTCGTG GCAGCAGTGG CGGCGATGTT TGTCGGCTCG GGATGGGTCC 101 AGGATGTTAC TCCTTCTTCT TTTGTTGGGG TCTGGGCAGG GGCCACAGCA 151 AGTCGGGGCG GGTCAAACGT TCGAGTACTT GAAACGGGAG CACTCGCTGT 201 CGAAGCCCTA CCAGGGTGTG GGCACAGGCA GTTCCTCACT GTGGAATCTG 251 ATGGGCAATG CCATGGTGAT GACCCAGTAT ATCCGCCTTA CCCCAGATAT 301 GCAAAGTAAA CAGGGTGCCT TGTGGAACCG GGTGCCATGT TTCCTGAGAG 351 ACTGGGAGTT GCAGGTGCAC TTCAAAATCC ATGGACAAGG AAAGAAGAAT 401 CTGCATGGGG ATGGCTTGGC AATCTGGTAC ACAAAGGATC GGATGCAGCC 451 AGGGCCTGTG TTTGGAAACA TGGACAAATT TGTGGGGCTG GGAGTATTTG 501 TAGACACCTA CCCCAATGAG GAGAAGCAGC AAGAGCGGGT ATTCCCCTAC 551 ATCTCAGCCA TGGTGAACAA CGGCTCCCTC AGCTATGATC ATGAGCGGGA 601 TGGGCGGCCT ACAGAGCTGG GAGGCTGCAC AGCCATTGTC CGCAATCTTC 651 ATTACGACAC CTTCCTGGTG ATTCGCTACG TCAAGAGGCA TTTGACGATA 701 ATGATGGATA TTGATGGCAA GCATGAGTGG AGGGACTGCA TTGAAGTGCC 751 CGGAGTCCGC CTGCCCCGCG GCTACTACTT CGGCACCTCC TCCATCACTG 801 GGGATCTCTC AGATAATCAT GATGTCATTT CCTTGAAGTT GTTTGAACTG 851 ACAGTGGAGA GAACCCCAGA AGAGGAAAAG CTCCATCGAG ATGTGTTCTT 901 GCCCTCAGTG GACAATATGA AGCTGCCTGA GATGACAGCT CCACTGCCGC 951 CCCTGAGTGG CCTGGCCCTC TTCCTCATCG TCTTTTTCTC CCTGGTGTTT 1001 TCTGTATTTG CCATAGTCAT TGGTATCATA CTCTACAACA AATGGCAGGA 1051 ACAGAGCCGA AAGCGCTTCT ACTGAGCCCT CCTGCTGCCA CCACTTTTGT 1101 GACTGTCACC CATGAGGTAT GGAAGGAGCG GGCACTGGCC TGAGCATGCA 1151 GCCTGGAGAG TGTTCTTGTC TCTAGCAGCT GGTTGGGGAC TATATTCTGT 1201 CACTGGAGTT TTGAATGCAG GGACCCCGCA TTCCCATGGT TGTGCATGGG 1251 GACATCTAAC TCTGGTCTGG GAAGCCACCC ACCCCAGGGC AATGCTGCTG 1301 TGATGTGCCT TTCCCTGCAG TCCTTCCATG TGGGAGCAGA GGTGTGAAGA 1351 GAATTTACGT GGTTGTGATG CCAAAATCAC GGAACAGAAT TTCATAGCCC 1401 AGGCTGCCGT GTTGTTTGAC TCAGAAGGCC CTTCTACTTC AGTTTTGAAT 1451 CCACAAAGAA TTAAAAACTG GTAACACCAC AGGCTTTCTG ACCATCCATT 1501 CGTTGGGTTT TGCATTTGAC CCAACCCTCT GCCTACCTGA GGAGCTTTCT 1551 TTGGAAACCA GGATGGAAAC TTCTTCCCTG CCTTACCTTC CTTTCACTCC 1601 ATTCATTGTC CTCTCTGTGT GCAACCTGAG CTGGGAAAGG CATTTGGATG 1651 CCTCTCTGTT GGGGCCTGGG GCTGCAGAAC ACACCTGCGT TTCGCTGGCC 1701 TTCATTAGGT GGCCCTAGGG AGATGGCTTT CTGCTTTGGA TCACTGTTCC 1751 CTAGCATGGG TCTTGGGTCT ATTGGCATGT CCATGGCCTT CCCAATCAAG 1801 TCTCTTCAGG CCCTCAGTGA AGTTTGGCTA AAGGTTGGTG TAAAAATCAA 1851 GAGAAGCCTG GAAGACACCA TGGATGCCAT GGATTAGCTG TGCAACTGAC 1901 CAGCTCCAGG TTTGATCAAA CCAAAAGCAA CATTTGTCAT GTGGTCTGAC 1951 CATGTGGAGA TGTTTCTGGA CTTGCTAGAG CCTGCTTAGC TGCATGTTTT 2001 GTAGTTACGA TTTTTGGAAT CCCTCTTTGA GTGCTGAAAG TGTAAGGAAG 2051 CTTTCTTCTT ACACCTTGGG CTTGGATATT GCCCAGAGAA GAAATTTGGC 2101 TTTTTTTTCT TAATGGACAA GGGACAGTTG CTGTTCTCAT GTTCCAAGTC 2151 TGAGAGCAAC AGACCCTCAT CATCTGTGCC TGGAAGAGTT CACTGTCATT 2201 GAGCAGCACA GCCTGAGTGC TGGCCTCTGT CAACCCTTAT TCCACTGCCT 2251 TATTTGACAA GGGGTTACAT GCTGCTCACC TTACTGCCCT GGGATTAAAT 2301 CAGTTACAGG CCAGAGTCTC CTTGGAGGGC CTGGAACTCT GAGTCCTCCT 2351 ATGAACCTCT GTAGCCTAAA TGAAATTCTT AAAATCACCG ATGGAACCAA 2401 AAAAAAAAAA AAAAAA BLAST Results Entry HS622145 from database EMBL: human STS WI-6746. Score = 1079, P = 5.1e-43, identities = 219/223 Entry G42541 from database EMBLNEW: SHGC-58649 Human Homo sapiens STS genomic, sequence tagged site. Score = 1091, P = 1.7e-43, identities = 219/220 Medlme entries 94265253: A putative novel class of animal lectins m the secretory pathway homologous to leguminous lectins . 94208543: VIP36, a novel component of glycolipid rafts and exoeytic carrier vesicles in epithelial cells. Peptide information for frame 2 ORF from 29 bp to 1072 bp; peptide length: 348 Category: strong similarity to known protein 1 MAATLGPLGS WQQWRRCLSA RDGSRMLLLL LLLGSGQGPQ QVGAGQTFEY 51 LKREHSLSKP YQGVGTGSSS LWNLMGNAMV MTQYIRLTPD MQSKQGALWN 101 RVPCFLRDWE LQVHFKIHGQ GKKNLHGDGL AIWYTKDRMQ PGPVFGNMDK 151 FVGLGVFVDT YPNEEKQQER VFPYISAMVN NGSLSYDHER DGRPTELGGC 201 TAIVRNLHYD TFLVIRYVKR HLTIMMDIDG KHEWRDCIEV PGVRLPRGYY 251 FGTSSITGDL SDNHDVISLK LFELTVERTP EEEKLHRDVF LPSVDNMKLP 301 EMTAPLPPLS GLALFLIVFF SLVFSVFAIV IGIILYNKWQ EQSRKRFY BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_23124, frame 2 PIR:G01447 GP36b glycoprotein - human, N = 1, Score = 1001, P = 5.9e-101 SWIΞSPROT:VP36_CANFA VESICULAR INTEGRAL-MEMBRANE PROTEIN VIP36 PRECURSOR (VIP36)., N = 1, Score = 990, P = 8.6e-100 TREMB :CET04G9_2 gene: "T04G9.3"; Caenorhabditis elegans cosmid T04G9., N = 1, Score = 614, P = 6e-60 PIR:S42626 ER-golgi intermediate compartment protein - human, N = 2, Score = 397, P = le-42 >PIR:G01447 GP36b glycoprotein - human Length = 356 HSPs: Score = 1001 (150.2 bits), Expect = 5.9e-101, P = 5.9e-101 Identities = 197/356 (55%), Positives = 256/356 (71%) Query: 1 MAATLGPLGSWQQWRRCLSARDG SRMLLLLLLLGSGQGPQQVGAGQTFEYLK 52 MAA G + W RRCL R G + L LLLLLGS + G + E+LK Sbjct: 1 MAAE-GWIWRWGWGRRCLG-RPGLLGPGPGPTTPLFLLLLLGΞVTA--DITDGNS-EHLK 55 Query: 53 REHΞLSKPYQGVGTGSSSLWNLMGNAMVMTQYIRLTPDMQSKQGALWNRVPCFLRDWELQ 112 REHSL KPYQGVG+ S LW+ G+ M+ +QY+RLTPD +SK+G++WN PCFL+DWE+ Sbjct: 56 REHSLIKPYQGVGSSSMPLWDFQGSTMLTSQYVRLTPDERSKEGSIWNHQPCFLKDWEMH 115 Query: 113 VHFKIHGQGKKNLHGDGLAIWYTKDRMQPGPVFGNMDKFVGLGVFVDTYPNEEKQQERVF 172 VHFK+HG GKKNLHGDG+A+WYT+DR+ PGPVFG+ D F GL +F+DTYPN+E ERVF Sbjct: 116 VHFKVHGTGKKNLHGDGIALWYTRDRLVPGPVFGSKDNFHGLAIFLDTYPNDETT-ERVF 174 Query: 173 PYISAMVNNGSLSYDHERDGRPTELGGCTAIVRNLHYDTFLVIRYVKRHLTIMMDIDGKH 232 PYIS MVNNGSLSYDH +DGR TEL GCTA RN +DTFL +RY + LT+M D++ K+ Sbjct: 175 PYISVMVNNGSLSYDHSKDGRWTELAGCTADFRNRDHDTFLAVRYSRGRLTVMTDLEDKN 234 Query: 233 EWRDCIEVPGVRLPRGYYFGTSSITGDLSDNHDVISLKLFELTVERTPEEEKLHRDVFLP 292 EW++CI++ GVRLP GYYFG S+ TGDLSDNHD+IS+KLF+L VE TP+EE + P Sbjct: 235 EWKNCIDITGVRLPTGYYFGASAGTGDLSDNHDIISMKLFQLMVEHTPDEESIDWTKIEP 294 Query: 293 SVDNMKLPEMTAPLP PLSGLALFLIVFFSLVFSVFAIVIGIILYNKWQEQSRK 345 SV+ +K P+ P PL+G +FL++ +L+ V V+G +++ K QE++ K Sbjct: 295 SVNFLKSPKDNVDDPTGNFRΞGPLTGWRVFLLLLCALLGIVVCAVVGAVVFQKRQERN-K 353 Query: 346 RFY 348 RFY Sbjct: 354 RFY 356 Pedant information for DKFZphfbr2_23124, frame 2 Report for DKFZphfbr2_23124.2 [LENGTH] 348 [MW] 39711.10 [pi] 8.55 [HOMOL] PIR:G01447 GP 3 6b glycoprotein human le-101 [PIRKW] lectin 2e-37 [PIRKW] transmembrane protein 2e-37 [PIRKW] endoplasmic reticulum 2e-37 [PIRKW] Golgi apparatus 2e-37 [PROSITE] AMIDATION 1 [PROSITE] MYRISTYL 5 [PROSITE] CK2_PHOSPHO_SITE 2 [PROSITE] GLYCOSAMINOGLYCAN 1 [PROSITE] PKC_PHOSPHO_SITE 3 [PROSITE] ASN_GLYCOSYLATION 1 [KW] Alpha_Beta [KW] SIGNAL_PEPTIDE 39 [KW] LOW COMPLEXITY 7.76 % SEQ MAATLGPLGSWQQWRRCLSARDGΞRMLLLLLLLGSGQGPQQVGAGQTFEYLKREHSLSKP SEG xxxxxxx PRD ccccccccccccccccccccccchhhhhhhhhhhcccccccccccchhhhhhhhhhhccc SEQ YQGVGTGSSSLWNLMGNAMVMTQYIRLTPDMQSKQGALWNRVPCFLRDWELQVHFKIHGQ SEG PRD cccccccccceeecccccccccceeeeccchhhhhcccccccccchhhhhhhheeeeecc SEQ GKKNLHGDGLAIWYTKDRMQPGPVFGNMDKFVGLGVFVDTYPNEEKQQERVFPYISAMVN SEG PRD ccccccccceeeeeecccccccccccccccccceeeeeecccccccccccccceeeeeec SEQ NGSLSYDHERDGRPTELGGCTAIVRNLHYDTFLVIRYVKRHLTIMMDIDGKHEWRDCIEV SEG PRD ccccccccccccccccccccccccccccccceeeehhhhhhheeeeeccccccccccccc SEQ PGVRLPRGYYFGTSSITGDLSDNHDVISLKLFELTVERTPEEEKLHRDVFLPΞVDNMKLP SEG PRD cccccccccccccccccccccccchhhhhhhhhhhhhccccccccccccccccccccccc SEQ EMTAPLPPLSGLALFLIVFFSLVFSVFAIVIGIILYNKWQEQSRKRFY SEG xxxxxxxxxxxxxxxxxxxx PRD cccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccc Prosite for DKFZphfbr2_23124.2 PS00001 181->185 ASN_GLYCOSYLATION PDOC00001 PS00002 35->39 GLYCOSAMINOGLYCAN PDOC00002 PS00005 19->22 PKC PHOSPHO SITE PDOC00005 PS00005 268->271 PKC_PHOSPHO_SITE PDOC00005 PS00005 343->346 PKC_PHOSPHO_SITE PDOC00005 PS00006 19->23 CK2_PHOSPHO_SITE PDOC00006 PS00006 279->283 CK2_PHOSPHO_SITE PDOC00006 PS00008 43->49 MYRISTYL PDOC00008 PS00008 63->69 MYRISTYL PDOC00008 PΞ00008 65->71 MYRISTYL PDOC00008 PS00008 96->102 MYRISTYL PDOC00008 PS00008 198->204 MYRISTYL PDOC00008 PS00009 120->124 AMIDATION PDOC00009 (No Pfam data available for DKFZphfbr2_23124.2) DKFZphfbr2_23nl6 group: signal transduction DKFZphfbr2_23nl6.1 encodes a novel 292 ammo acid protein with weak similarity to putative phosphatιdylιnosιtol-4-phosphate 5-kmase of Arabidopsis thaliana. The novel proteins contains a WW domain which has been originally described as a short conserved region in a number of unrelated proteins, among them dystrophin, the gene responsible for Duchenne muscular dystrophy. The domain, which spans about 35 residues, is repeated up to 4 times m some proteins. It has been shown to bind proteins with particular proline-motifs, [AP] -P-P- [AP] -Y, and thus resembles somewhat SH3 domains. This domain is frequently associated with other domains typical for proteins in signal transduction processes. Examples of proteins containing the WW domain are Dystrophin, Utrophin, vertebrate YAP protein (binds the SH3 domain of the Yes oncoprotein) , murine NEDD-4 (embryonic development and differentiation of the central nervous system) , IQGAP (human GTPase activating protein acting on ras) . Therefore the new protein should be involved in intracellular signal transduction. The new protein can find application in modulating/blocking intracellular signal transduction pathways . similarity to putative phosphatιdylιnosιtol-4-phosphate 5-kιnase complete cDNA, complete eds, EST hits Sequenced by AGOWA Locus: unknown Insert length: 2936 bp Poly A stretch at pos. 2916, polyadenylation signal at pos. 2873 1 GGGGGCGCTC CCGAGAAAGA GTGAGGGCGC GACGCGCACC AACGGTGGAG 51 GGATGTTTCA GCAGCCCCTG AGAAGGAAGA GGAGGAAGCT GAGGGCCCGC 101 TGAGGGCGCA GGACCTGAGG GAGTCCTACA TCCAGCTCGT CCAGGGTGTG 151 CAGGAGTGGC AGGATGGTTG CATGTACCAG GGGGAGTTTG GGTTGAACAT 201 GAAGCTTGGA TATGGCAAAT TCTCTTGGCC CACAGGCGAG TCATACCATG 251 GGCAGTTTTA CCGGGACCAC TGCCATGGCC TGGGTACCTA CATGTGGCCA 301 GATGGCTCCA GTTTCACGGG CACATTTTAC CTCAGCCACC GAGAAGGCTA 351 CGGCACCATG TACATGAAGA CACGGCTTTT CCAGACTCAC TGCCACAACG 401 ACATTGTCAA CCTTCTCCTG GACTGTGGGG CCGACGTGAA CAAGTGCTCA 451 GATGAGGGTC TCACGGCACT CAGCATGTGT TTCCTCCTCC ACTACCCCGC 501 CCAGTCCTTC AAGCCCAATG TTGCTGAACG GACCATACCT GAGCCCCAGG 551 AACCTCCAAA ATTCCCAGTT GTTCCAATCC TTTCATCATC ATTTATGGAC 601 ACAAACCTGG AGTCTCTGTA CTATGAGGTG AACGTGCCTT CCCAGGGTAG 651 CTATGAGCTG AGGCCACCGC CAGCACCACT GCTCCTGCCA CGCGTCTCAG 701 GCAGCCACGA GGGCGGCCAC TTCCAGGACA CCGGGCAGTG TGGGGGGTCC 751 ATAGACCACA GGAGCAGCTC TCTGAAGGGG GACTCCCCGT TGGTGAAGGG 801 CAGCCTTGGC CATGTGGAAA GCGGGCTTGA GGACGTGTTG GGAGACACAG 851 ACCGGGGCAG TCTGTGCAGT GCTGAGACGA AATTTGAGTC CAACTTGTGT 901 GTGTGCGACT TCTCCATCGA GCTCTCGCAG GCCATGCTGG AGAGAAGCGC 951 CCAGTCCCAC AGCTTGCTGA AGATGGCCTC GCCCTCACCG TGCACCAGCA 1001 GCTTCGACAA AGGGACCATG CGGAGGATGG CGCTGTCCAT GATCGAGTAG 1051 GTCCTGGCAC CAGCTGGTGG GGGTGGAGGG CCACCATCAG GGCTGAATCC 1101 TATGCTCAGC AGACCCACGT CTCTTCCCTG TGCCAGTGGG AGGCGTTGTG 1151 TCTGGAGATG TGTGTCTGAA TGTGTGAGCA TCCCTGTGTC GGTGGCTCCA 1201 TGCCATGGCC AGCCCTGTGG GGGTGCCACG GTGACGGGCT GTTTTCAGTG 1251 CCACCCCAGC CCTGTGGGGG TGCCACGGTG ACGGGCTGTT TTCAGTACCA 1301 CGCCAGCCCT GCTTTGGCCT TTGGCACTGG CCTGAAGTGT CTCTGTGGGA 1351 GCCTCAGCAG GGGCCACTGT CAGGGGTCCT ATCCTAGCCA TAGTGCACGT 1401 GAGTGACACC TGCCTGGGCA GCTCTCACAC CCCTGCTGTC CACCCTGTCT 1451 ATACCAGTGT GTCTCAAAAT GTGGTCTATG CACCCCCGGG GGTCCAAGAC 1501 CCTTTCAGGG AGTCTGTGGG GTCAAAATGA TTCTCTTGAT AACCCTGAGA 1551 CTCTGTTAGC CTTCTCCTTG TGTTGATGTT GGTGGATGGT ATGAAGACAG 1601 GGCCGTGCAG ACCACCAGCC CCCAGCGTGC AGGGCAGCAG TGCCCGGCCT 1651 GCTTGGGGGC ATGGTATTCC TTCACCACGG TGTGCACTTG CGGGGATGCC 1701 TGTCTCACTG AAGAATGCCT TTGACTAAGC AGAAAAGCAA TGACAAATTG 1751 CATTAAATCT TGCTCCTTGC GTACACACCC CTCGAATATT CTGGGTCGGA 1801 AAACATGGGA AGGACACTGA TGTGTGTCTG CCACAGACCA AGGCACACCG 1851 CTTCCCCGCA AGAAGCGCTT CCCCCAGGGC CAGAGTAGCA ACAGAATGCG 1901 GCATCTTCCC AACCTCCTGC CCCATTTTTG ATTGGAAGAA TGACCACTGG 1951 TATGTGGCTG TTCATTCTCC TGAACACAGC CTGCCACTTT AAGGAAAACA 2001 TATGACACTA TTTGTTGCTG GCGAAATTTA CATTTTCAAG TGAATAGCAG 2051 AATTCTGGAC ACTTGCCACC ACCACCAAAA CCTTCATAGC TTCCCTTAAC 2101 TTTGAGACAT GGGTGTTCAG AGGTTTTTCA CGTGAGATGG CGTTAGCAGC 2151 GCAGTTTTGT GATACTGCCT GAAGACATGC CGACAGTGCC CAGATCTCTT 2201 CTATTGGTGA GCCAGCTTTT CCCACACGGC CAAGTTCTGA TGTTGAACCA 2251 TTGCCAGGTG GGTGAAGATC CATTGACAGT GAGAGGTGGG CCCGTGGGCT 2301 TCAGTGCAGC CAGGCGCAGA AGGCTGGTTC ATGAGTGTCC AGCTCCGCCA 2351 GGTAGCTAGC TCACCACCCC CAGCCTGGGT TCATGTAGTT CAAATAGGAA 2401 GACCACGATG ATCAGAAAGG CTGCTCAAAT ACTCCTTCGT CCAGCCGCGT 2451 ACCTGGGGGA GGCTGAATCT CCACTCACTT CCACCAAGGC TGTGCAGAGC 2501 AGATAGGGGA ATCCAGCAAA GGTGGAAAAC AGTGCCATCC TTCTCCCCAA 2551 CTGGTTTTGT TTTGTAAAAT AACTTTTTGT GACAGTGTTA CTTATTAGTA 2601 ACATGCAGTG GGTTTGTTAT GGTTAACAAG TTGGTGAGCA TTATTGAGAG 2651 GTGAAGCCAG CTGAGCTTCT GGGTTGGGTG GGGACTTGGA GAACTTTTGT 2701 GTCTAGCTAA AGGATTGTAA ATGCACCAAT CAATGCTCAG TGTCTAGCTA 2751 AAGGATTGTA AATGCACCAA TCAGCACTCT GTAAAATTGA CCAATCAGCG 2801 TTCTGTAAAA TGGACCAATC AGTGGTCTGT AAAATGGACC AGTCAGCAGG 2851 ATGTGGGCGG GGCCAAAAAA GGGAATAAAA GCTGGCCACC GCCAGGCTCC 2901 CCACCAGCCT GCAGCGAAAA AAAAAAAAAA AAAAAA BLAST Results No BLAST result Medlme entries No Medlme entry Peptide information for frame 1 ORF from 172 bp to 1047 bp; peptide length: 292 Category: similarity to unknown protein Prosite motifs: WW DOMAIN 1 (19-24) 1 MYQGEFGLNM KLGYGKFSWP TGESYHGQFY RDHCHGLGTY MWPDGSSFTG 51 TFYLSHREGY GTMYMKTRLF QTHCHNDIVN LLLDCGADVN KCSDEGLTAL 101 SMCFLLHYPA QSFKPNVAER TIPEPQEPPK FPVVPILSSS FMDTNLESLY 151 YEVNVPSQGS YELRPPPAPL LLPRVSGSHE GGHFQDTGQC GGSIDHRSSS 201 LKGDSPLVKG SLGHVESGLE DVLGDTDRGS LCSAETKFES NLCVCDFSIE 251 LSQAMLERSA QSHSLLKMAS PSPCTSSFDK GTMRRMALSM IE BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_23nl6, frame 1 TREMBL:AB005902_1 product: "AtPIP5Kl"; Arabidopsis thaliana mRNA for AtPIP5Kl, complete eds., N = 2, Score = 138, P = 1. le-06 TREMBL :AF019380_1 product: "putative phosphatιdylιnosιtol-4-phosphate 5-kmase"; Arabidopsis thaliana putative phosphatιdylmosιtol-4-phosphate 5-kιnase mRNA, complete eds., N = 2, Score = 138, P = 1.4e-06 PIR:T02098 probable phosphatιdylιnosιtol-4-phosphate 5-kιnase - Arabidopsis thaliana, N = 2, Score = 135, P = 6.7e-06 >TREMBL:AB005902_1 product: "AtPIP5Kl"; Arabidopsis thaliana mRNA for AtPIP5Kl, complete eds. Length = 683 HSPs: Score = 138 (20.7 bits), Expect = 1. le-06, Sum P(2) = 1. le-06 Identities = 23/61 (37%), Positives = 35/61 (57%) Query: 1 MYQGEFGLNMKLGYGKFSWPTGESYHGQFYRDHCHGLGTYMWPDGSSFTGTFYLSHREGY 60 MY+G++ G GKFSWP+G +Y G+F G GT+ DG ++ GT+ + G+ Sbjct: 34 MYEGDWKRGKASGKGKFSWPSGATYEGEFKSGRMEGFGTFTGADGDTYRGTWVADRKHGH 93 Query: 61 G 61 G Sbjct: 94 G 94 Score = 112 (16.8 bits), Expect = 9.7e-04, Sum P(2) = 9.7e-04 Identities = 19/51 (37%), Positives = 27/51 (52%) Query: 12 LGYGKFSWPTGESYHGQFYRDHCHGLGTYMWPDGSSFTGTFYLSHREGYGT 62 +G GK+ W G Y G + R G G + WP G+++ G F EG+GT Sbjct: 22 IGSGKYLWKDGCMYEGDWKRGKASGKGKFSWPSGATYEGEFKSGRMEGFGT 72 Score = 97 (14.6 bits), Expect = 4.4e-02, Sum P(2) = 4.3e-02 Identities = 19/60 (31%), Positives = 32/60 (53%) Query: 2 YQGEFGLNMKLGYGKFSWPTGESYHGQFYRDHCHGLGTYMWPDGSSFTGTFYLSHREGYG 61 Y+GEF G+G F+ G++Y G + D HG G + +G + GT+ + ++G G Sbjct: 58 YEGEFKSGRMEGFGTFTGADGDTYRGTWVADRKHGHGQKRYANGDFYEGTWRRNLQDGRG 117 Score = 93 (14.0 bits), Expect = 1.2e-01, Sum P(2) = l.le-01 Identities = 18/62 (29%), Positives = 34/62 (54%) Query: 2 YQGEFGLNMKLGYGKFSWPTGESYHGQFYRDHCHGLGTYMWPDGSSFTGTFYLSHREGYG 61 Y+G + + K G+G+ + G+ Y G + R+ G G Y+W +G+ +TG + + G G Sbjct: 81 YRGTWVADRKHGHGQKRYANGDFYEGTWRRNLQDGRGRYVWRNGNQYTGEWRIGVISGKG 140 Query: 62 TM 63 + Sbjct: 141 LL 142 Score = 91 (13.7 bits), Expect = 2.0e-01, Sum P(2) = 1.8e-01 Identities = 18/51 (35%), Positives = 24/51 (47%) Query: 2 YQGEFGLNMKLGYGKFSWPTGESYHGQFYRDHCHGLGTYMWPDGSSFTGTF 52 Y GE+ + + G G WP G Y G + G G + W DGSS G + Sbjct: 127 YTGEWRIGVISGKGLLVWPNGNRYEGLWENGIPKGNGVFTWSDGSSCVGAW 177 Score = 90 (13.5 bits), Expect = 2.6e-01, Sum P(2) = 2.3e-01 Identities = 17/60 (28%), Positives = 31/60 (51%) Query: 2 YQGEFGLNMKLGYGKFSWPTGESYHGQFYRDHCHGLGTYMWPDGSΞFTGTFYLSHREGYG 61 Y+G + N++ G G++ W G Y G++ G G +WP+G+ + G + +G G Sbjct: 104 YEGTWRRNLQDGRGRYVWRNGNQYTGEWRIGVISGKGLLVWPNGNRYEGLWENGIPKGNG 163 Score = 45 (6.8 bits), Expect = 1. le-06, Sum P(2) = 1. le-06 Identities = 14/62 (22%), Positives = 26/62 (41%) Query: 215 VESGLEDVLGDTDRGSLCSAETKFESNLCVCDF—SIELSQAMLERSAQSHSLLKMASPS 272 V+SG + G+ +C E+ E+ CD ++E S +R + + + Sbjct: 205 VDSGAGSLGGEKVFPRICIWESDGEAGDITCDIIDNVEASMIYRDRISVDRDGFRQFKKN 264 Query: 273 PC 274 PC Sbjct: 265 PC 266 Pedant information for DKFZphfbr2_23nl6, frame 1 Report for DKFZphfbr2_23nl6.1 [LENGTH] 292 [MW] 32214.44 [pi] 5.51 [HOMOL] TREMBL:AB005902_1 product: "AtPIP5Kl"; Arabidopsis thaliana mRNA for AtPIP5Kl, complete eds. 7e-08 [BLOCKS] BL01137A Hypothetical YBL055c/y jV family proteins [PROSITE] WW_DOMAIN_l 1 [PROSITE] MYRISTYL 5 [PROSITE] CK2_PHOSPHO_SITE 7 [PROSITE] PKC_PHOSPHO_SITE 5 [KW] Alpha_Beta [KW] LOW_COMPLEXITY 4.11 % SEQ MYQGEFGLNMKLGYGKFSWPTGESYHGQFYRDHCHGLGTYMWPDGSSFTGTFYLSHREGY SEG PRD cccccccccccccccceeeccccccccccccccccccccccccccccccceeeeeccccc SEQ GTMYMKTRLFQTHCHNDIVNLLLDCGADVNKCSDEGLTALSMCFLLHYPAQSFKPNVAER SEG PRD cccchhhhhheeeccccchhhhhcccccccccccccchhhhhhhhhccccccccccceee SEQ TIPEPQEPPKFPVVPILSSSFMDTNLESLYYEVNVPSQGSYELRPPPAPLLLPRVSGSHE SEG xxxxxxxxxxx PRD eccccccccceeeeeeeccccccccccceeeeeecccccccccccccccccccccccccc SEQ GGHFQDTGQCGGSIDHRSSSLKGDSPLVKGSLGHVESGLEDVLGDTDRGSLCSAETKFES SEG PRD cccccccccccccccccccccccccceeecccccccccccccccccccccceeeeecccc SEQ NLCVCDFSIELSQAMLERSAQSHSLLKMASPSPCTSSFDKGTMRRMALSMIE SEG PRD cccccchhhhhhhhhhhhhhhhhhhhcccccccccccccccchhhhhhhccc Prosite for DKFZphfbr2_23nl6.1 PS00005 55->58 PKC PHOSPHO SITE PDOC00005 PS00005 112->115 PKC PHOSPHO- " SITE PDOC00005 PΞ00005 200->203 PKC PHOSPHO " " SITE PDOC00005 PS00005 226->229 PKC PHOSPHO " " SITE PDOC00005 PS00005 282->285 PKC PHOSPHO " SITE PDOC00005 PS00006 55->59 CK2 PHOSPHO " " SITE PDOC00006 PS00006 121->125 CK2 PHOSPHO " SITE PDOC00006 PS00006 140->144 CK2 PHOSPHO " " SITE PDOC00006 PS00006 144->148 CK2 PHOSPHO " " SITE PDOC00006 PS00006 217->221 CK2 PHOSPHO " " SITE PDOC00006 PS00006 236->240 CK2 PHOSPHO " " SITE PDOC00006 PS00006 276->280 CK2 PHOSPHO " " SITE PDOC00006 PS00008 45->51 MYRISTYL PDOC00008 PS00008 86->92 MYRISTYL PDOC00008 PS00008 177->183 MYRISTYL PDOC00008 PS00008 188->194 MYRISTYL PDOC00008 PS00008 229->235 MYRISTYL PDOC00008 PS01159 19->44 WW DOMAIN 1 PDOC50020 (No Pfam data available for DKFZphfbr2_23nl6.1) DKFZphfbr2_23o24 group: brain derived DKFZphfbr2_23o24 encodes a novel 139 amino acid protein with similarity to CAAX-box proteins. The CAAX box is a prenyl group binding site found in a number of eukaryotic proteins, such as which is found in Ras- and ras-like proteins such as Rho, Rab, Rac, Ral, and Rap, as well as in nuclear lamins A and B, some G protein alpha and gamma subunits and some dnaJ-like proteins. These proteins are posttranslationally modified at this site by the attachment of either a farnesyl or a geranyl-geranyl group to a cysteine residue. No informative BLAST results; no predictive prosite, pfam or SCOP motife The new protein can find application in studying the expression profile of brain-specific genes . similarity to lectins complete cDNA, complete eds, EST hits Sequenced by AGOWA Locus: unknown Insert length: 3564 bp Poly A stretch at pos. 3541, no polyadenylation signal found 1 GAATGGCTCC GCAGATGGCC GGCACTGAGA GCCAGCAAGA AGCGGAGGAG 51 ATGGGCCTTC AGCAGGGGGT TGCGGGGGGA GCTTTAAACT GAGCCCTGTA 101 AACATGGCAG AACTGCTCAG TGGGAGACTC TCAGCACAGA CGGTCATGGG 151 GAAGTGAGTG CAGTTCATTT GTAATCTTGT TGTCGAGTTC TGGGTTTTTT 201 TTGTTTGTTT CGTAACTTTA AAGGTATGCA CTTTATATAG ATTTATTTAT 251 TTGCTGGGAC CGTTACTCAG AGTTCCTAGA AATGTACACA GCTTTTTTAC 301 CAGGGTTACT CCTCAGAATC ACTTGTCACT TCTTTAAATG AATGAATGAA 351 TGTGCCAGGC CCTATGCCTG GAGGTTGGGA GCTTCATCTA CATCACATTC 401 TAACAGGTGA CCACTGGGGT AAGCACTGTG TGACTGCAAA GCCAGGGTGT 451 GTTTCCATCA ACACCCAGAT GACCGTGCCT ATGTGCCCCT GTTGTCCTCC 501 CTCCAGGACT GCCTCCTCAC CCCACCCCTT TCTGCAGCTC CTCATCTAAA 551 CATCTCGCCT GGTGAGGTCA CGGCTTAGCC TGTTGGCCAG TGGCCCCACC 601 ACCATCCTTC CCCCTGTGCA GATTGGAGGA GGCCAGGTCT CTCCCCTTAG 651 CTCCTATGTC CCCTTCACCC CCCATGGCAC AGATGAGACA TTCACAGAGT 701 TTGCAGATGA TGGAAGAGAA GACTCCAGGT TGCCAGGTGT GTCCACTCTC 751 AGGAACCCCC AGCCCAAGCC TCACTGCTCG TGTTCCCAGC CAACCCCAGC 801 ACGGGGGATA CGCCGGTGCT GTTTCCCTGC TCAGATACAA CCAGTTACCA 851 GAAACGACCT CACCCCTCCA ACCACTTTCC AAGGTGCCAG GACAGAGAAG 901 CCCTTCACTG GCCCACCCAG GGCAGTTGAC AGAGGGATGC CCTCCTTGGA 951 GGGGAGCCTC ACCTCTACCC ACAGGGCCGC GGCCTTGTCC TGGATTCTCA 1001 CCGGGGCAGT CACGTCAGGA TGGAGAGGTC CCATGTCAGC CAGTTCTTTG 1051 GTGGGGGTCA TGTAGTCTGA AATGACCTGC CGATGGTCCA GGCTGAGCCA 1101 GGGAAGCTGA GCCTGGGTGC CTTTTTGGTG CCTACTCTGA CTTGAGTTGG 1151 ATTCATGCCA CAGACCCACC TTCTTGAGCA ACAACACATA TAGCCACCAA 1201 CACAAGAGCC AGGCACACAC TGAGCAGAGA AAGTCCCTGT CGCCTCACCA 1251 CCCAAAAACT CCAGCTTTGC AGAGACCAAG GTTCTTCTCT ACCTTTGCAG 1301 AAGCCTCTGT GACCAAACCC GGAGCTTGCC CTTCTGAGGC CTCTAGCATT 1351 TCTCCAGGTG TTTTTCAGAG GACTTGGTTT AAATTTGTTC ACCCCAAATG 1401 TGGTCTTTCC CGGATCATGA AAGGATCTGC CGCAAAGGTG AATCTGAGTC 1451 TCCTCAGAGT CATATGAGAC TGAAACTGCT TATAACATTT CCGTGACCTA 1501 ATAAGTCTTC CAAAAATGTA GGGTATTAAG AGTTTAGTGA CATTAAAAAG 1551 TTTAGTCGAA AATATCGTGA TTCAGGTATA TTTAGACATT TGATTCATGC 1601 CAAATTGCCA CTGTTAACAG AAAACACACC CCAAGCACAT TAATGCCTAG 1651 ATATTTCAAA CCCTTTTCTG CCCACACATT CTTAAAAATA ATATACTGAG 1701 AAATCTATAT ACAGGTTTTT TTTTAATTAG CTTGGAAAAG AGCAGTTGTA 1751 TTCTGTTTGA ACAGCTGCTA ATGTCAATTC CTGTGGGAAG AAAGACCAAA 1801 GAACATGGAG TTACACCAAG AATTTTAAAA CAAAGACGCT GTCCCTTTCC 1851 TGAGCACCGT GCAGCCAAGA CTGAGAGATC AGTCTGAGAC CTGTGATTAA 1901 GGAGTGTTTT CTACATAGCG TATAATTATG GAGCCACACA AGTGGGCCAT 1951 TACTCTGTTG AGTGCTTCAT GTTTGAGGTA TTTTCGTGTT CCAACTTACA 2001 TTAAAGTGTT TATAAAACAG GAAAAATCCA CGAGCAGGTA TTGACACTAT 2051 CCATATTAGA TCATCACAAA ATTATATATA TAGCAGAGTC ATAAACAATG 2101 AGAAACGGTC TTCCCACACT TGCTTTAAAT GGCCATGACC TAGTGTTTAG 2151 GGAAAGCAGT AAAATCAGCG AGGAGCTCGT GGGAAAAATG AGACGGGCCC 2201 TGAGGGGGTG ACTCATGGGC CAAGCAGGGC CACACAGGTA CCAGGCCGCC 2 251 ACGTCCTCTC CTGCCTCTCA CTCTCTGGAG ACTGGACTTC CTTTACTGCC 2301 TCCTTTCTGA CATTTCCTAG ACATCAGACT TTGCTACTTA GTACACAAAC 2351 GGGGTTCCCT TTTAAATTTG TTCACTCTAG TTAGCATTTG CAGAAGCTGT 2401 GAAAAATTAC AGAGAGATGA TGTGTTGGGT AAGAGATGGT TTAAAAGTCC 2451 AGCTTGCTGT TTTTCATTAA GTGTCTTGAA AATGAGTAAG TGGCGTTCCT 2501 GGAGGGGAAC AATCATATAA TTCCGCAGGG TGGGTCTAAA CTTGTTTTCT 2551 GATAGTGTTT AGCAGCTCAT GGCTCTGAGG GCACCTGATA ACACAGCAGC 2601 CAGGCGCTGA TGAGAAGTGT GTGCCAGACA GACCCGAGTG TGGCTTGGCT 2651 CTTGCCTTAT GTTCCTTTCT CTGTTCAGAG AAGCGTGAGA TGAGATTTTG 2701 TGATTATATT GCACTCCTTG GGCTGACTTT CCCATGCACA GAATGTTTTA 2751 CACATCCTGA TAGCTGAGCT GAAAATGCAA AGAGAAGGGA AAATGCCTTA 2801 AATTGTTCTG GCTAATTTAG AAGCAGCAGG CCTTGGAAGT CTTTGTCCTG 2851 TGTCCCTGAA CAAATCTTAT GGGAGCTCTG GTACCTATGC CAGAAAATGC 2901 ACATAGGCAC AACACTTTTA CATACACGTT CACACACCCC ACCCTTATGG 2951 AGAACTTTTT TCTAAATAAG AGAAAGAAAA ATTTTAAGAC TTACAAGTTA 3001 TGTTTAGGTA TTTTACATGG TTCAGAAAAC AAGACATGAA GCGGTATAAA 3051 CTGAGAAGTC TTGTTCCCAC AACCCCACGT GCCAGGTACA CATAACCATT 3101 TTTATTCACC TCTAGCTTGT GCTTCCAATG TTTGTTAGGC ATATGTAAAT 3151 AAGTGAATAG ATAAGCATTT CTCCCTCCTT TTGCTGACAT GAGTGGTGGC 3201 ATGTTTTGCC CCTGGCTTTT ATCCCTTGAC CCCATTCCAG TACCTAGAGA 3251 CCTGCTTCAT TTTTTTAGAT GTGTAATACT TCATGTGTGC GTGTGCCTTA 3301 GTGATTAACT CGTGCACTGT GCAGGGACAT CGGGCTGGGA TCAGTTTGTT 3351 CACTGATATA TACAGCGCTG CGGGAGATAC CCTCACATGT GTATCATTTG 3401 GTCCATGTGC AGGTGTGTCT GGAAGATAGA ATTCTAGGCG TAGAATTGAT 3451 AGGTTAAATG TATTTATAGG GAAAAAATCA ATATAAAACT TTGCGTGTAA 3501 TGATATTTGC GTGCTTTTTT TTTTAATTTT TTTACCCAAA TAGTAAAAAA 3551 AAAAAAAAAA AAAA BLAST Results No BLAST result Medline entries No Medlme entry Peptide information for frame 2 ORF from 656 bp to 1072 bp; peptide length: 139 Category: similarity to known protein 1 MSPSPPMAQM RHSQSLQMME EKTPGCQVCP LSGTPSPSLT ARVPSQPQHG 51 GYAGAVSLLR YNQLPETTSP LQPLSKVPGQ RSPSLAHPGQ LTEGCPPWRG 101 ASPLPTGPRP CPGFSPGQSR QDGEVPCQPV LWWGSCSLK BLASTP hits Entry CEEGAP7_1 from database TREMBL: gene: "EGAP7.1"; Caenorhabditis elegans cosmid EGAP7. Score = 123, P = 2.3e-07, identities = 35/103, positives = 44/103 Entry MMBPC35_1 from database TREMBL: Mouse carbohydrate binding protein 35 mRNA, 3 ' end. Score = 113, P = 2.2e-06, identities = 40/103, positives = 44/103 Entry A28651 from database PIR: galactose-specific lectin - mouse >TREMB : MMMAC2A_1 Mouse mRNA for Mac-2 antigen Score = 113, P = 2.2e-06, identities = 40/103, positives = 44/103 Alert BLASTP hits for DKFZphfbr2_23o24, frame 2 No Alert BLASTP hits found Pedant information for DKFZphfbr2_23o24, frame 2 Report for DKFZphfbr2_23o24.2 [LENGTH] 139 [MW] 14748.91 [pi] 8.90 [PROSITE] PRENYLATION 1 [PROSITE] MYRISTYL 1 [PROSITE] CK2_PHOSPHO_SITE 1 [PROSITE] PROKAR_LIPOPROTEIN 1 [PROSITE] PKC_PHOSPHO_SITE 1 [KW] All_Alpha SEQ MSPSPPMAQMRHSQSLQMMEEKTPGCQVCPLSGTPSPSLTARVPSQPQHGGYAGAVSLLR PRD cccchhhhhhhhhhhhhhhhhccccccccccccccccccccccccccccccchhhhhhhh SEQ YNQLPETTSPLQPLSKVPGQRSPSLAHPGQLTEGCPPWRGASPLPTGPRPCPGFSPGQSR PRD hhcccccccccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ QDGEVPCQPVLWWGSCSLK PRD ccccccccccccccccccc Prosite for DKFZphfbr2_23o24.2 PS00005 40->43 PKC_PHOSPHO_SITE PDOC00005 PS00006 119->123 CK2_PHOSPHO_SITE PDOC00006 PS00008 50->56 MYRISTYL PDOC00008 PS00013 126->137 PROKAR_LIPOPROTEIN PDOC00013 PS00294 136->140 PRENYLATION PDOC00266 (No Pfam data available for DKFZphfbr2_23o24.2) DKFZphfbr2_23o5 group: brain derived DKFZphfbr2_23o5 encodes a novel 360 ammo acid protein with no known similarity No informative BLAST results; no predictive prosite, pfam or SCOP motife The new protein can find application in studying the expression profile of brain-specific genes . unknown potential start at Bp 24 matchs Kozak consensus ANNatgG Sequenced by AGOWA Locus: /map="7q21-q22" Insert length: 1736 bp Poly A stretch at pos. 1714, polyadenylation signal at pos. 1680 1 GGGGGAGGAT CAAAGTAGGC AAGATGGCGT CGAGCGGCGG GGAGCCAGGG 51 AGTTTATTTG ATCACCACGT CCAGAGGGCG GTATGCGACA CACGGGCCAA 101 ATATCGAGAG GGACGACGGC CTCGTGCTGT GAAGGTATAT ACAATCAATT 151 TGGAATCTCA GTACTTATTA ATACAAGGAG TTCCTGCTGT GGGAGTCATG 201 AAGGAATTAG TTGAGCGATT CGCTTTATAT GGTGCAATTG AACAGTACAA 251 TGCTCTAGAT GAATACCCAG CAGAAGACTT TACTGAAGTT TATCTTATTA 301 AATTTATGAA CTTACAAAGT GCAAGGACAG CCAAGAGAAA AATGGATGAA 351 CAGAGTTTCT TCGGTGGATT GCTTCATGTG TGCTATGCTC CAGAATTTGA 401 AACAGTTGAA GAAACTAGAA AAAAACTACA AATGCGGAAG GCATATGTAG 451 TAAAAACTAC TGAAAATAAA GACCATTACG TGACAAAGAA GAAATTGGTT 501 ACAGAGCATA AAGACACAGA GGATTTTAGA CAAGACTTCC ACTCAGAGAT 551 GTCTGGATTT TGTAAAGCTG CTTTGAACAC TTCTGCAGGG AACTCAAATC 601 CTTATCTTCC GTATTCCTGT GAATTGCCTT TATGTTATTT CTCCTCAAAA 651 TGTATGTGTT CATCCGGGGG ACCTGTAGAC AGAGCACCAG ACTCCTCTAA 701 GGATGGTAGA AACCATCATA AAACAATGGG GCATTATAAC CACAATGACT 751 CTTTGCGGAA AACACAGATA AACTCTTTGA AAAACTCAGT GGCCTGCCCT 801 GGTGCACAAA AGGCTATTAC GTCTTCAGAG GCAGTTGACA GATTTATGCC 851 TAGGACAACA CAACTGCAGG AGCGCAAAAG AAGAAGAGAA GATGATCGTA 901 AACTTGGAAC TTTTCTTCAA ACAAACCCAA CTGGTAATGA GATTATGATT 951 GGACCTCTGT TACCAGACAT CTCTAAAGTG GATATGCACG ATGACTCATT 1001 GAATACAACG GCGAATTTAA TTCGGCATAA ACTTAAAGAG GTATTTCATC 1051 TGTGCCAAAG CCTCCAGAGG ACAAGCCAGA AGATGTACAT ACAAGTCATC 1101 CATTAAAACA AAGAAGAAGA ATATAGAGTG CCAGCAGCAA CTTAGTATTT 1151 TCTAAAAAGA ACATTTATTA TTTATTTTTA GCCTGTCATT TTAATTCTTC 1201 AAGAGATTTT ACTGCTGGTA TTTTTTGATG CACTCCTCTT TGTAATTTCA 1251 TTCAAGCCAT TTGTCTAAAG TCATTTCTTT GTTTTTTGGG AGATGGAGTC 1301 TTGCTCTGTT GCCCAGGCTG GAATGCAGTG GCGTGATCTC GGCTCACTGC 1351 AACCTCCACC TCCCGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCTGAGT 1401 ATCTGGGATT ACAGGCGTGC ACCACCATGC CTGGCTAAGT TTTGTGTTTT 1451 TTTTAGTAGA GATGGGTTTT CACCATATTG GTCAGGCTGG TCTCGAACTC 1501 CTGACCTTGT GATACACCTG CCTCAGCCTC CCAAAGGGAT GAGCCACCGC 1551 GCCTGGCCCA TTTCTTCTTT TTTTGACCCA TACTTAATGT TGCAGAAACT 1601 ATTCTTGTCA TAACATTATC TCTCATGTAC AGTAATTATA TGTAAATTAA 1651 TTGAAGCAAA TATGGAAACT TTACAATAGA AATAAAGATA GGCAGCCAGC 1701 GTCTGTTTCC AATTATAAAA AAAAAAAAAA AAAAAA BLAST Results Entry AC005156 from database EMBL: Homo sapiens PAC clone DJ1099C19 from 7q21-q22, complete sequence. Score = 2897, P = 2.4e-154, identities = 583/586 2 exons covering Bp 465-17 3 Medline entries No Medline entry Peptide information for frame 3 ORF from 24 bp to 1103 bp; peptide length: 360 Category: similarity to unknown protein 1 MASSGGEPGS LFDHHVQRAV CDTRAKYREG RRPRAVKVYT INLESQYLLI 51 QGVPAVGVMK ELVERFALYG AIEQYNALDE YPAEDFTEVY LIKFMNLQSA 101 RTAKRKMDEQ SFFGGLLHVC YAPEFETVEE TRKKLQMRKA YVVKTTENKD 151 HYVTKKKLVT EHKDTEDFRQ DFHSEMSGFC KAALNTΞAGN SNPYLPYSCE 201 LPLCYFSSKC MCSSGGPVDR APDSSKDGRN HHKTMGHYNH NDSLRKTQIN 251 SLKNSVACPG AQKAITSΞEA VDRFMPRTTQ LQERKRRRED DRKLGTFLQT 301 NPTGNEIMIG PLLPDISKVD MHDDSLNTTA NLIRHKLKEV FHLCQSLQRT 351 SQKMYIQVIH BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_23o5, frame 3 TREMBL:AC005824_10 gene: "F15K20.11"; Arabidopsis thaliana chromosome II BAC F15K20 genomic sequence, complete sequence., N = 2, Score = 114, P = 3.6e-ll >TREMBL:AC005824_10 gene: "F15K20.11"; Arabidopsis thaliana chromosome II BAC F15K20 genomic sequence, complete sequence. Length = 227 HSPs: Score = 114 (17.1 bits), Expect = 3.6e-ll, Sum P(2) = 3.6e-ll Identities = 21/41 (51%), Positives = 29/41 (70%) Query: 103 AKRKMDEQSFFGGLLHVCYAPEFETVEETRKKLQMRKAYVV 143 AKRK+DE SF G L + YAPE+E V +T+ KL+ R+ V+ Sbjct: 51 AKRKLDESΞFLGNRLQISYAPEYENVNDTKDKLESRRKEVL 91 Score = 107 (16.1 bits), Expect = 2.6e-10, Sum P(2) = 2.6e-10 Identities = 50/191 (26%), Positives = 83/191 (43%) Query: 103 AKRKMDEQSFFGGLLHVCYAPEFETVEETRKKLQMRKAYVVKTTENKDHYVTKKKLVTEH 162 AKRK+DE SF G L + YAPE+E V +T+ KL+ R+ V+ + T + VT+ Sbjct: 51 AKRKLDESSFLGNRLQISYAPEYENVNDTKDKLESRRKEVLARLNPQKEKSTSQ—VTKL 108 Query: 163 KDTEDFRQDFHSEMΞGFCKAALNTSAGNSNPYLPYSCELPLCYFSSKCMCSSGGPVDRAP 222 + D S + + GN+ P S + YF+S M + V Sbjct: 109 AGPALTQTDNVSSQRREMEYQFHR—GNA-PVTRVSSDQE—YFASSSMNQTVKTV 159 Query: 223 DSSKDGRNHHKTMGHYNHNDSLRKTQINSLKNSVACPGAQKAITSSEAVDRFMPRTTQLQ 282 K + + + +H + ++ N + P +Q S R P ++Q+Q Sbjct: 160 -REKLNKTREENISSLSHCKQIEESG-NQKRLQ PSSQTQPEESGNQKRLQP-SSQIQ 213 Query: 283 -ERKRRREDDRK 293 + KR R D+R+ Sbjct: 214 PDLKRTRVDNRR 225 Score = 102 (15.3 bits), Expect = 3.6e-ll, Sum P(2) = 3.6e-ll Identities = 22/55 (40%), Positives = 38/55 (69%) Query: 26 KYREGRRPRAVKVYTINLESQYLLIQGVPAVGVMKELVERFALYGAIEQY—NALDE 80 +Y++ P AV+VYT+ ES+Y++++ VPA+G +L+ F YG +E++ LDE Sbjct: 3 RYKD-ETP-AVRVYTVCDESRYMIVRNVPALGCGDDLMRLFMTYGEVEEFAKRKLDE 57 Pedant information for DKFZphfbr2_23o5, frame 3 Report for DKFZphfbr2_23o5.3 [LENGTH] 360 [MW] 41105.85 [pi] 8.89 [HOMOL] TREMBL:AC005824_10 gene: "F15K20.11"; Arabidopsis thaliana chromosome II BAC F15K20 genomic sequence, complete sequence. 5e-12 [PROSITE] AMIDATION 1 [PROSITE] MYRISTYL 2 [PROSITE] CK2 PHOSPHO SITE 7 [PROSITE] PKC_PHOSPHO_SITE 9 [PROSITE] ASN_GLYCOSYLATION 3 [KW] Alpha_Beta [KW] LOW COMPLEXITY 4.17 SEQ MASSGGEPGSLFDHHVQRAVCDTRAKYREGRRPRAVKVYTINLESQYLLIQGVPAVGVMK SEG PRD ccccccccceeeecceeeeehhhhhhhhhccccceeeeeeecccceeeeeeccccchhhh SEQ ELVERFALYGAIEQYNALDEYPAEDFTEVYLIKFMNLQSARTAKRKMDEQSFFGGLLHVC SEG PRD hhhhhhhhhhhhhhhhhhccccccceeeeeeehhhhhhhhhhhhhhhhhccccccceeee SEQ YAPEFETVEETRKKLQMRKAYVVKTTENKDHYVTKKKLVTEHKDTEDFRQDFHSEMΞGFC SEG PRD eccchhhhhhhhhhhhhhhhheeeeccccceeeeeeeeeeeccccchhhhhhhhhcccce SEQ KAALNTSAGNSNPYLPYSCELPLCYFSSKCMCΞSGGPVDRAPDSSKDGRNHHKTMGHYNH SEG PRD eeeeccccccccccccccccccceeecccccccccccccccccccccccccccccccccc SEQ NDSLRKTQINSLKNSVACPGAQKAITSSEAVDRFMPRTTQLQERKRRREDDRKLGTFLQT SEG xxxxxxxxxxxxxxx PRD cccceeeeccccccccccccceeeeecceeeeeccccchhhhhhhhhhhhccceeeeeec SEQ NPTGNEIMIGPLLPDISKVDMHDDSLNTTANLIRHKLKEVFHLCQSLQRTSQKMYIQVIH SEG PRD cccccceeeecccccccccccccccccchhhhhhhhhhhhhhhhhhhhhcchhhhhhccc Prosite for DKFZphfbr2_23o5.3 PS00001 185-M89 ASN_GLYCOSYLATION PDOC00001 PS00001 241->245 ASN_GLYCOSYLATION PDOC00001 PS00001 327->331 ASN_GLYCOSYLATION PDOC00001 PS00005 99->102 PKC_PHOSPHO_SITE PDOC00005 PS00005 102->105 PKC_PHOSPHO_SITE PDOC00005 PS00005 131->134 PKC_PHOSPHO_SITE PDOC00005 PS00005 154->157 PKC_PHOSPHO_SITE PDOC00005 PS00005 207->210 PKC_PHOSPHO_SITE PDOC00005 PS00005 224->227 PKC_PHOSPHO_SITE PDOC00005 PS00005 243->246 PKC_PHOSPHO_SITE PDOC00005 PS00005 251->254 PKC_PHOSPHO_SITE PDOC00005 PS00005 351->354 PKC_PHOSPHO_SITE PDOC00005 PS00006 4->8 CK2_PHOSPHO_SITE PDOC00006 PS00006 10->14 CK2_PHOSPHO_SITE PDOC00006 PS00006 127->131 CK2_PHOSPHO_SITE PDOC00006 PS00006 224->228 CK2_PHOSPHO_ΞITE PDOC00006 PS00006 266->270 CK2_PHOSPHO_SITE PDOC00006 PS00006 303->307 CK2_PHOSPHO_SITE PDOC00006 PS00006 317->321 CK2_PHOSPHO_SITE PDOC00006 PS00008 5->ll MYRISTYL PDOC00008 PS00008 260->266 MYRISTYL PDOC00008 PS00009 29->33 AMIDATION PDOC00009 (No Pfam data available for DKFZphfbr2_23o5.3) DKFZphfbr2_2a2 group: brain derived DKFZphfbr2_2a2.3 encodes a novel 167 amino acid protein with weak similarity to human 52K autoantigen Ro/SS-A The novel protein contains a C3HC4 Zinc finger "RING finger" motive. This domain is probably involved in mediating protein-protein interactions. Proteins containing a RING-finger are: mammalian V(D)J recombination activating protein (RAG1), mouse rpt-1, human rfp, human 52 Kd Ro/SS-A protein and others. No informative BLAST results; no predictive prosite, pfam or SCOP motife The new protein can find application in studying the expression profile of bram-specific genes . similarity to 52K autoantigen Ro/SS-A - human complete cDNA, complete eds, few EST hits Sequenced by Qiagen Locus : unknown Insert length: 1376 bp Poly A stretch at pos. 1355, polyadenylatioif signal at pos. 1340 1 GGGGACTCCA AATTAGAAAG GGGACGTCTA GTGGGTTGCC CGGGAGGGGT 51 GGCGGGAGCG GTCCTGGAAA TAATCTGTCC TCTGTCGCCG GGAACTGGCG 101 AGGTAGTTCC TTCGCGGTGG AGAGACCTGG AATGGCCAAA TATCAAGGTG 151 AAGTTCAAAG TTTGAAACTG GATGATGATT CAGTTATAGA AGGAGTAAGC 201 GACCAAGTAC TTGTGGCAGT TGTGGTCAGT TTCGCTTTGA TTGCTACCCT 251 GGTATATGCA CTTTTCAGAA ATGTACATCA AAACATTCAC CCAGAAAACC 301 AGGAGCTAGT AAGGGTACTT CGAGAACAGC TTCAAACAGA ACAGGATGCA 351 CCTGCTGCCA CTCGACAGCA GTTCTACACT GACATGTACT GTCCCATCTG 401 CCTGCACCAA GCCTCCTTCC CGGTGGAGAC CAACTGTGGA CATCTTTTTT 451 GTGGTGCCTG CATTATTGCT TACTGGCGAT ATGGTTCATG GCTTGGGGCA 501 ATCAGTTGTC CAATCTGTAG ACAAACGGTA ACCTTACTCC TAACAGTATT 551 TGGTGAAGAT GATCAGTCTC AGGATGTTCT GAGATTGCAT CAGGATATTA 601 ATGATTATAA CCGGAGATTC TCAGGGCAAC CCTGATCTAT TATGGAGAGA 651 ATTATGGATC TACCCACTTT ACTGAGGCAT GCATTCAGGG AAATGTTTTC 701 AGTCGGGGGC CTTTTCTGGA TGTTTCGCAT CAGGATAATA CTTTGTTTAA 751 TGGGAGCTTT TTTCTATCTT ATATCACCTC TAGATTTTGT ACCTGAAGCC 801 TTGTTTGGAA TTCTAGGCTT TCTAGATGAT TTCTTTGTCA TCTTTTTATT 851 GCTTATCTAC ATCTCTATTA TGTATCGAGA AGTGATAACC CAAAGGCTAA 901 CTAGATGAAA AAGGAAACAA AACTGAGTTT ACTAGGATAT CTGAGCTAAT 951 GTAGAACATC AAACAGAAGG ACCCATGGCA GTATAAAGCA ATGAAGCAAT 1001 GGAGTATTAT CTCACAAATA TAAAACCACT ATAAGACAAA CATTTGATTA 1051 TCATTTGACA AATACCTAGG TATAACTGGA ATTTTCATGT TTGAAGTTCT 1101 AATATTAAGT TTAGAATTAT AATGATCTAC AGTTGTATCT TGATTCTATG 1151 TTGTCTGGAA AAAATATGGA ATTATATAAA AAGGGATGCT TTTATATATT 1201 TTTCTTTTCC CCAGAATTAC TTAGATTAAT TAGATGTATA GTAAAATATT 1251 GTTAAATGTC AGTTTATCCA TCTTATCCTT CTCAGCAGGT ACCTATATGA 1301 TAATATATAG CTGTGAAACT CATCTAAATA TTTTTGTTCC AATAAAATAT 1351 TATATACTAA AAAAAAAAAA AAAAAA BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 3 ORF from 132 bp to 632 bp; peptide length: 167 Category: similarity to known protein Classification: unset Prosite motifs: ZINC FINGER C3HC4 (102-112) 1 MAKYQGEVQS LKLDDDSVIE GVSDQVLVAV VVSFALIATL VYALFRNVHQ 51 NIHPENQELV RVLREQLQTE QDAPAATRQQ FYTDMYCPIC LHQASFPVET 101 NCGHLFCGAC IIAYWRYGSW LGAISCPICR QTVTLLLTVF GEDDQSQDVL 151 RLHQDINDYN RRFSGQP BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_2a2, frame 3 TREMBL :CEY38F1A_8 gene: "Y38F1A.2"; Caenorhabditis elegans cosmid Y38F1A, N = 1, Score = 194, P = 2e-15 PIR:T05222 hypothetical protein F17I5.130 - Arabidopsis thaliana, N = 1, Score = 159, P = 1.4e-10 TREMBLNEW:AB025011_1 gene: "TRIF"; product: "Trif-d"; Mus musculus mRNA for Tπf-d, complete eds., N = 1, Score = 108, P = 2.6e-06 PIR:A37241 52K autoantigen Ro/SS-A - human, N = 1, Score = 115, P = 5e-05 >TREMBL:CEY38F1A_8 gene: "Y38F1A.2"; Caenorhabditis elegans cosmid Y38F1A Length = 283 HSPs: Score = 194 (29.1 bits), Expect = 2.0e-15, P = 2.0e-15 Identities = 52/149 (34%), Positives = 78/149 (52%) Query: 16 DSVIEGVSDQVLVAVVVSFALIATLVYALFRNVHQNIHPENQELVRVLREQLQTEQDAPA 75 D +E ++ Q+ +A+ V F ++ + A Q E R Q+ T++ Sbjct: 41 DPDVE-LATQITMAIAVIF-IVKAIFDAWQSRRRQRAASRMDENAE--RNQIITQRRISE 96 Query: 76 ATRQQFYTDMYCPICLHQASFPVETNCGHLFCGACIIAYWRYGSWLGA-ISCPICRQTVT 134 A Q + CPICL AΞFPV T+CGH+FC CII YW+ + C +CR T Sbjct: 97 ALHQSSHE CPICLANASFPVLTDCGHIFCCECIIQYWQQΞKAIVTPCDCAMCRSTFY 153 Query: 135 LLLTV FGEDDQSQDVLRLHQ-DINDYNRRFS 164 +LL V G +++ D ++ + I+DYNRRFS Sbjct: 154 MLLPVHWPTMGTSEETDDHIQENNIRIDDYNRRFS 188 Pedant information for DKFZphfbr2_2a2, frame 3 Report for DKFZphfbr2_2a2.3 [LENGTH] 167 [MW] 18941.65 [pi] 4.91 [HOMOL] TREMBL:CEY38F1A_8 gene: "Y38F1A.2" Caenorhabditis elegans cosmid Y38F1A le-13 [FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YDR265w] le-04 [FUNCAT] 30.19 peroxisomal organization [S. cerevisiae, YDR265w] le-04 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YLR323c] 2e-04 [BLOCKS] BL00518 Zinc finger, C3HC4 type, proteins [PROSITE] ZINC_FINGER_C3HC4 1 [PFAM] Zinc finger, C3HC4 type (RING finger) [KW] Irregular [KW] 3D [KW] LOW COMPLEXITY 6.59 % SEQ MAKYQGEVQSLKLDDDSVIEGVSDQVLVAVVVSFALIATLVYALFRNVHQNIHPENQELV SEG xxxxxxxxxxx lrmd- SEQ RVLREQLQTEQDAPAATRQQFYTDMYCPICLHQASFPVETNCGHLFCGACIIAYWRYGSW SEG lrmd HHHHHHBTTTTTEETTTEEEETTTEEEEHHHHH HHHHH SEQ LGAISCPICRQTVTLLLTVFGEDDQSQDVLRLHQDINDYNRRFSGQP SEG lrmd- HCCB-TTTTT. Prosite for DKFZphfbr2_2a2.3 PS00518 102->112 ZINC FINGER C3HC4 PDOC00449 Pfam for DKFZphfbr2_2a2.3 HMM_NAME Zinc fmger, C3HC4 type (RING finger) HMM *CPICFcTFQlDyPWPFdePmMlPCgHsFCypCIrrW CP CPIC L+ P++++CGH+FC +CI+ + CP Query 87 CPIC LHQ ASFPVETNCGHLFCGACIIAYWRYGSWLGAISCP 127 HMM mC* +C Query 128 IC 129 DKFZphfbr2_2bl7 group: transmembrane protein DKFZphfbr2_2bl7 encodes a novel 285 amino acid protein with similarity to D. melanogaster 30K protein . The protein contains 3 transmembrane regions. No informative BLAST results; no predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of brain-specific genes and as a new marker for neuronal cells. similarity to Drosophila hypothetical 30K protein complete cDNA, complete eds, EST hits TRANSMEMBRANE 3 Sequenced by Qiagen Locus : unknown Insert length: 1426 bp Poly A stretch at pos. 1345, polyadenylation signal at pos. 1330 1 GGGGGTATTT CCAAGGACTC CAAAGCGAGG CCGGGGACTG AAGGTGTGGG 51 TGTCGAGCCC TCTGGCAGAG GGTTAACCTG GGTCAAATGC ACGGATTCTC 101 ACCTCGTACA GTTACGCTCT CCCGCGGCAC GTCCGCGAGG ACTTGAAGTC 151 CTGAGCGCTC AAGTTTGTCC GTAGGTCGAG AGAAGGCCAT GGAGGTGCCG 201 CCACCGGCAC CGCGGAGCTT TCTCTGTAGA GCATTGTGCC TATTTCCCCG 251 AGTCTTTGCT GCCGAAGCTG TGACTGCCGA TTCGGAAGTC CTTGAGGAGC 301 GTCAGAAGCG GCTTCCCTAC GTCCCAGAGC CCTATTACCC GGAATCTGGA 351 TGGGACCGCC TCCGGGAGCT GTTTGGCAAA GATGAACAGC AGAGAATTTC 401 AAAGGACCTT GCTAATATCT GTAAGACGGC GGCTACAGCA GGCATCATTG 451 GCTGGGTGTA TGGGGGAATA CCAGCTTTTA TTCATGCTAA ACAACAATAC 501 ATTGAGCAGA GCCAGGCAGA AATTTATCAT AACCGGTTTG ATGCTGTGCA 551 ATCTGCACAT CGTGCTGCCA CACGAGGCTT CATTCGTTAT GGCTGGCGCT 601 GGGGTTGGAG AACTGCAGTG TTTGTGACTA TATTCAACAC AGTGAACACT 651 AGTCTGAATG TATACCGAAA TAAAGATGCC TTAAGCCATT TTGTAATTGC 701 AGGAGCTGTC ACGGGAAGTC TTTTTAGGAT AAACGTAGGC CTGCGTGGCC 751 TGGTGGCTGG TGGCATAATT GGAGCCTTGC TGGGCACTCC TGTAGGAGGC 801 CTGCTGATGG CATTTCAGAA GTACTCTGGT GAGACTGTTC AGGAAAGAAA 851 ACAGAAGGAT CGAAAGGCAC TCCATGAGCT AAAACTGGAA GAGTGGAAAG 901 GCAGACTACA AGTTACTGAG CACCTCCCTG AGAAAATTGA AAGTAGTTTA 951 CAGGAAGATG AACCTGAGAA TGATGCTAAG AAAATTGAAG CACTGCTAAA 1001 CCTTCCTAGA AACCCTTCAG TAATAGATAA ACAAGACAAG GACTGAAAGT 1051 GCTCTGAACT TGAAACTCAC TGGAGAGCTG AAGGGAGCTG CCATGTCCGA 1101 TGAATGCCAA CAGACAGGCC ACTCTTTGGT CAGCCTGCTG ACAAATTTAA 1151 GTGCTGGTAC CTGTGGTGGC AGTGGCTTGC TCTTGTCTTT TTCTTTTCTT 1201 TTTAACTAAG AATGGGGCTG TTGTACTCTC ACTTTACTTA TCCTTAAATT 1251 TAAATACATA CTTATGTTTG TATTAATCTA TCAATATATG CATACATGAA 1301 TATATCCACC CACCTAGATT TTAAGCAGTA AATAAAACAT TTCGCAAAAG 1351 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 1401 AAAAAAAAAA AAAAAAAAAA AAAAAA BLAST Results Entry HSG19630 from database EMBL: human STS A001T27. Score = 961, P = 1.2e-36, identities = 193/194 Medlme entries No Medlme entry Peptide information for frame 3 ORF from 189 bp to 1043 bp; peptide length: 285 Category: similarity to unknown protein 1 MEVPPPAPRS FLCRALCLFP RVFAAEAVTA DSEVLEERQK RLPYVPEPYY 51 PESGWDRLRE LFGKDEQQRI SKDLANICKT AATAGIIGWV YGGIPAFIHA 101 KQQYIEQSQA EIYHNRFDAV QSAHRAATRG FIRYGWRWGW RTAVFVTIFN 151 TVNTSLNVYR NKDALSHFVI AGAVTGSLFR INVGLRGLVA GGIIGALLGT 201 PVGGLLMAFQ KYSGETVQER KQKDRKALHE LKLEEWKGRL QVTEHLPEKI 251 ESSLQEDEPE NDAKKIEALL NLPRNPSVID KQDKD BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_2bl7, frame 3 PIR:JQ1024 hypothetical 30K protein (DmRP140 5' region) - fruit fly (Drosophila melanogaster), N = 1, Score = 312, P = 6.1e-28 >PIR:JQ1024 hypothetical 30K protein (DmRP140 5' region) - fruit fly (Drosophila melanogaster) Length = 261 HSPs: Score = 312 (46.8 bits), Expect = 6.1e-28, P = 6.1e-28 Identities = 68/231 (29%), Positives = 125/231 (54%) Query: 30 ADSEVLEERQKRLPYVPEPYYPESGWDRLRELFGKDEQQRISKDLANICKTAATAGIIGW 89 AD V +E + ++ E+G +RL+++F DE I +L ++ + +IG Sbjct: 23 ADEIVDKENKTYKAFLASKPPEETGLERLKQMFTIDEFGSIFSELNSVYQAGFLGFLIGA 82 Query: 90 VYGGIPAFIHAKQQYIEQSQAEIYHNRFDAVQSAHRAATRGFIRYGWRWGWRTAVFVTIF 149 +YGG+ A ++E +QA + + FDA + T F + G++WGWR +F T + Sbjct: 83 IYGGVTQSRVAYMNFMENNQATAFKSHFDAKKKLQDQFTVNFAKGGFKWGWRVGLFTTSY 142 Query: 150 NTVNTSLNVYRNKDALSHFVIAGAVTGSLFRINVGLRGLVAGGIIGALLGTPVGGLLMAF 209 + T ++VYR K ++ ++ AG++TGSL+++++GLRG+ AGGIIG LG G + Sbjct: 143 FGIITCMSVYRGKSSIYEYLAAGSITGSLYKVSLGLRGMAAGGIIGGFLGGVAGVTSLLL 202 Query: 210 QKYSGETVQERKQKDRKALHELKLEEWKGRLQVTEHLPEKIESSLQEDEPE 260 K SG +++E ++ ++K RL E++ + + +++ PE Sbjct: 203 MKASGTSMEE VRYWQYKWRLDRDENIQQAFKKLTEDENPE 242 Pedant information for DKFZphfbr2_2bl7, frame 3 Report for DKFZphfbr2_2bl7.3 [LENGTH] 285 [MW] 32177.88 [pi] 8.65 [HOMOL] PIR:JQ1024 hypothetical 30K protein (DmRP140 5' region) - fruit fly (Drosophila melanogaster) 7e-20 [PROSITE] MYRISTYL 7 [PROSITE] CK2_PHOSPHO_SITE 5 [PROSITE] ASN_GLYCOSYLATION 1 [KW] SIGNAL_PEPTIDE 25 [KW] TRANSMEMBRANE 3 [KW] LOW_COMPLEXITY 5.96 % SEQ MEVPPPAPRSFLCRALCLFPRVFAAEAVTADSEVLEERQKRLPYVPEPYYPESGWDRLRE SEG PRD cccccccceeeeeeeeeehhhhhhhhhhhhhhhhhhhhhhhhhccccccccccchhhhhh MEM SEQ LFGKDEQQRISKDLANICKTAATAGIIGWVYGGIPAFIHAKQQYIEQSQAEIYHNRFDAV SEG PRD hhcccchhhhhhhhhhhhhhhhcccceeeeccccchhhhhhhhhhhhhhhhhhhhhhhhh MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM SEQ QSAHRAATRGFIRYGWRWGWRTAVFVTIFNTVNTSLNVYRNKDALSHFVIAGAVTGSLFR SEG PRD hhhhhhhhhhhccccccccceeeeeeeeccccccceeecccccccceeeeecccccceee MEM MMMMMMMMMMMMMMMMMMMMMMMMMM M SEQ INVGLRGLVAGGIIGALLGTPVGGLLMAFQKYSGETVQERKQKDRKALHELKLEEWKGRL SEG .. xxxxxxxxxxxxxxxxx PRD eecccccccccceeeeeccccccchhhhhhhhccchhhhhhhhhhhhhhhhhhhhhhhhh MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMM SEQ QVTEHLPEKIESSLQEDEPENDAKKIEALLNLPRNPSVIDKQDKD SEG PRD ccccccccchhhhhccccccchhhhhhhhhhcccccceeeccccc MEM Prosite for DKFZphfbr2_2bl7.3 PS00001 153->157 ASN_GLYCOSYLATION PDOC00001 PS00006 53->57 CK2_PHOSPHO_SITE PDOC00006 PS00006 108->112 CK2_PHOSPHO_SITE PDOC00006 PS00006 216->220 CK2_PHOSPHO_SITE PDOC00006 PS00006 253->257 CK2_PHOSPHO_SITE PDOC00006 PS00006 277->281 CK2_PHOSPHO_SITE PDOC00006 PS00008 92->98 MYRISTYL PDOC00008 PS00008 172->178 MYRISTYL PDOC00008 PS00008 187->193 MYRISTYL PDOC00008 PS00008 191->197 MYRISTYL PDOC00008 PS00008 195->201 MYRISTYL PDOC00008 PS00008 199->205 MYRISTYL PDOC00008 PΞ00008 204->210 MYRISTYL PDOC00008 (No Pfam data available for DKFZphfbr2_2bl7.3) DKFZphfbr2 2b5 group: cell structure and motility DKFZphfbr2_2b5 encodes a novel 957 amino acid protein with strong similarity to collagens. The novel protein contains the typical (xxG)n repeat of collagen proteins and a Pfam von Willebrand factor type A domain. Therefore, the protein seems to be a new collagen alpha chain. The new protein can find application in modulation of connective tissue, bone and cartilage development and mamtainance. similarity to collagen proteins shows typical (xxG)n repeat of collagen proteins [PFAM] von Willebrand factor type A domain Sequenced by Qiagen Locus: /map="6" Insert length: 4160 bp Poly A stretch at pos. 4141, polyadenylation signal at pos. 4119 1 GGGGGCCCGC TGCAGGGAGA ACGGACTCCG GGCGGAGGGC AGCCAATCCG 51 TTTCAGCGCA GGTCTTGCTC GGGTTGGGCT TGCCACTGCC TGGAACATAC 101 CTGTCCCCCT GGCGCAACAC TCAGCTGGCT GCGACCGCAA CCCCGAGCCT 151 GGACACTGCG CCAGGAATCC TAAAACCAAA ATATTAGAAC GAAAACAGAA 201 ACATGGCTCA CTATATTACA TTTCTCTGCA TGGTTTTGGT GCTGCTTCTT 251 CAGAATTCTG TGTTAGCTGA AGATGGGGAA GTAAGATCAA GTTGTCG AC 301 TGCTCCGACA GATTTAGTTT TCATCTTAGA TGGCTCTTAT AGTGTTGGCC 351 CAGAAAACTT TGAAATAGTG AAAAAGTGGC TTGTCAATAT CACAAAAAAC 401 TTTGACATAG GGCCGAAGTT TATTCAAGTT GGAGTGGTTC AATATAGTGA 451 CTACCCTGTG CTGGAGATTC CTCTCGGAAG CTATGATTCA GGAGAACATT 501 TGACGGCAGC AGTGGAATCC ATACTCTACT TAGGAGGAAA CACAAAGACA 551 GGGAAGGCCA TCCAGTTTGC GCTCGATTAC CTTTTTGACA AGTCCTCACG 601 ATTTCTGACT AAGATAGCAG TGGTACTTAC GGATGGCAAG TCCCAAGATG 651 ACGTCAAGGA TGCAGCTCAA GCAGCAAGAG ATAGTAAGAT AACATTATTT 701 GCTATTGGTG TTGGTTCAGA AACAGAAGAT GCCGAACTTA GAGCTATTGC 751 CAACAAGCCT TCGTCTACTT ATGTGTTTTA TGTGGAAGAC TATATTGCAA 801 TATCCAAAAT AAGGGAAGTG ATGAAGCAGA AACTTTGTGA AGAATCTGTC 851 TGTCCAACAC GAATTCCAGT GGCAGCTCGT GATGAAAGGG GATTTGATAT 901 TCTTTTGGGT TTAGATGTAA ATAAAAAGGT TAAGAAAAGA ATACAGCTTT 951 CACCAAAAAA GATAAAAGGA TATGAAGTAA CATCAAAAGT TGATTTATCA 1001 GAACTCACAA GCAATGTTTT CCCAGAAGGT CTTCCTCCAT CATATGTATT 1051 TGTGTCTACT CAAAGATTTA AAGTCAAGAA AATTTGGGAT TTATGGAGAA 1101 TATTAACTAT TGATGGAAGG CCACAAATAG CAGTTACCTT AAATGGTGTG 1151 GACAAAATCT TATTATTTAC AACAACCAGC GTAATTAATG GCTCACAAGT 1201 GGTTACCTTT GCTAACCCTC AAGTTAAGAC GTTGTTTGAT GAAGGCTGGC 1251 ACCAAATTCG TCTCTTAGTA ACAGAACAAG ATGTGACTTT GTATATTGAT 1301 GACCAACAAA TTGAAAACAA GCCCTTACAT CCAGTTTTAG GGATCTTGAT 1351 CAATGGGCAA ACCCAAATTG GAAAATATTC TGGAAAAGAA GAAACTGTTC 1401 AGTTTGATGT CCAAAAGTTG CGAATCTACT GTGACCCAGA ACAGAACAAC 1451 CGGGAGACAG CATGTGAGAT TCCTGGATTT AATGGAGAGT GCCTTAATGG 1501 TCCCAGTGAT GTAGGTTCAA CTCCAGCTCC CTGTATTTGT CCTCCGGGAA 1551 AACCAGGACT TCAAGGCCCC AAAGGTGACC CTGGACTGCC TGGGAACCCT 1601 GGCTACCCTG GACAACCTGG TCAAGATGGT AAGCCTGGAT ATCAGGGAAT 1651 TGCAGGGACA CCAGGTGTTC CAGGATCTCC AGGAATACAA GGAGCTCGAG 1701 GACTACCAGG TTACAAAGGA GAACCAGGGC GAGATGGTGA CAAGGGTGAT 1751 CGTGGACTTC CTGGTTTTCC TGGGCTTCAT GGCATGCCAG GATCAAAGGG 1801 TGAAATGGGT GCCAAAGGAG ACAAAGGATC ACCTGGATTT TATGGCAAAA 1851 AGGGTGCAAA AGGTGAAAAG GGGAATGCTG GCTTCCCTGG CCTCCCTGGA 1901 CCTGCTGGAG AACCAGGAAG ACATGGAAAG GATGGATTAA TGGGTAGTCC 1951 CGGTTTCAAG GGAGAAGCAG GATCCCCTGG TGCTCCGGGG CAGGATGGAA 2001 CACGGGGAGA GCCTGGAATC CCAGGATTTC CTGGAAACCG AGGATTAATG 2051 GGCCAAAAGG GAGAAATTGG GCCTCCAGGA CAGCAAGGAA AAAAAGGAGC 2101 CCCAGGGATG CCTGGTTTAA TGGGAAGCAA TGGCTCACCA GGCCAGCCTG 2151 GAACACCGGG ATCTAAGGGA AGCAAAGGTG AACCTGGAAT TCAAGGGATG 2201 CCTGGGGCTT CAGGGCTCAA GGGAGAACCA GGAGCAACGG GTTCCCCAGG 2251 AGAACCAGGA TACATGGGTT TACCCGGGAT TCAAGGAAAA AAGGGGGACA 2301 AAGGAAATCA AGGTGAAAAA GGTATTCAGG GTCAAAAGGG AGAAAATGGA 2351 AGACAGGGAA TTCCAGGGCA ACAGGGAATT CAAGGCCATC ATGGTGCAAA 2401 AGGAGAGAGA GGTGAAAAGG GAGAACCTGG TGTCCGAGGT GCCATTGGAT 2451 CAAAAGGAGA ATCTGGGGTG GATGGCTTGA TGGGGCCCGC AGGTCCTAAG 2501 GGGCAACCTG GGGATCCAGG TCCTCAGGGA CCCCCAGGTT TGGATGGGAA 2551 GCCCGGAAGA GAGTTTTCAG AACAATTTAT TCGACAAGTT TGCACAGATG 2601 TAATAAGAGC CCAGCTACCA GTCTTACTTC AGAGTGGAAG AATTAGAAAT 2651 TGTGATCATT GCCTGTCCCA ACATGGCTCC CCGGGTATTC CTGGGCCACC 2701 TGGTCCGATA GGCCCAGAGG GTCCCAGAGG ATTACCTGGT TTGCCAGGAA 2751 GAGATGGTGT TCCTGGATTA GTGGGTGTCC CTGGACGTCC AGGTGTCAGA 2801 GGATTAAAAG GCCTACCAGG AAGAAATGGG GAAAAAGGGA GCCAAGGGTT 2851 TGGGTATCCT GGAGAACAAG GTCCTCCTGG TCCCCCAGGT CCAGAGGGCC 2901 CTCCTGGAAT AAGCAAAGAA GGTCCTCCAG GAGACCCAGG TCTCCCTGGC 2951 AAAGATGGAG ACCATGGAAA ACCTGGAATC CAAGGGCAAC CAGGCCCCCC 3001 AGGCATCTGC GACCCATCAC TATGTTTTAG TGTAATTGCC AGAAGAGATC 3051 CGTTCAGAAA AGGACCAAAC TATTAGTGTC TGATGCCTCA TTCAGCAGCC 3101 TAGGCATGGT GCTTTTTCTG TGGTCTTTTG CATCTCAGGA AGATAACCAA 3151 CAGTATCCCT TGAAAAGAAA CTTAAGTACC TCGGTGTTTT TATTTTTTTT 3201 TTCTTATGGA AAAAAATATA AAAGATCACA TATACTGATT TTAAAGGCTC 3251 CTCAGTCATT TGGAGCCCTT GGATTAGCAG CATTAATTAA ATCTCAAGGG 3301 TTTCTTGTAA AGTCCATTTA TGTTAATCAA AGTTGAATAT AAAAATCCAC 3351 CATTGCCTGT TAGCCAGTCA GTTTTAGTCA CTGTGAAATA TTTCACATTC 3401 AGCCTCCATG CAGTAGAGAT TTGAGTTTAA TTTCATGTCC ATGTGACTTT 3451 CATGTTTCCT ATCTCATAGC TCATGCTACT ACATAAGCCA AAACATGTAT 3501 CTCATCATTG GAAGTAAGAT CAGGGCTGAT ATTCACCTGG GATAGACAGT 3551 ATTGGTGAAC TACTCATTTA CTACAGTGTC TCAGCCTTGA TAAAGGGCAG 3601 TGGATTGCCT GTTGTTCGGT GTTGTGAATA GCACCTCTGA ATAAGATTAG 3651 AGTGTTTCTT AATTCATTTC AAACTCTAAA ATTAGATTAA TGGTGGTGCT 3701 AAGAAAGAGT ATTAATTACT TTGGGAATGG TCAAAATTAA CATTAAAAAC 3751 ATTTTAGACA AAAAGTTTCA TTGTACATTC AAAGAAAATG TAAGTTTGGA 3801 AGTACTAAAA GACTATTTTA TACTTGTTGA TTAATCGGAA TGTTTGTTGT 3851 ATGCCTTCAT TTTCCATTTC ACTTATATGT GCATGTCCAT ATATGTTAAT 3901 TTTCATTGTA GCAAAGCTAA TGGAAATAAA GCTAATGCTC TAGTTGAAAG 3951 AAAAGGAAAA CTCCTGAAAT CCTAGAATGT CTTGTTATTT TTAGCTGACT 4001 GTAAAATATT ATGAACAGTC TTTGTGTATT GTGCTTAATG CTTTTGTAAG 4051 AAACAGAATT TGAAATATTT CATCCTTGTC ATGCTCAAAA TTTTGTTACA 4101 TGCTTGTTAT TCAGAGTATA ATAAAGTTTT GTACAGGCCT GAAAAAAAAA 4151 AAAAAAAAAA BLAST Results Entry HS682J15 from database EMBLNEW: Human DNA sequence *** SEQUENCING IN PROGRESS *** from clone 682J15 Score = 6240, P = O.Oe+00, identities = 1256/1263 13 exons matching Bp 2015-4118 Entry HS708F5 from database EMBLNEW: Human DNA sequence *** SEQUENCING IN PROGRESS *** from clone 708F5 Score = 2775, P = 1.0e-221, identities = 739/912 10 exons matching Bp 5-1745 Medline entries No Medlme entry Peptide information for frame 2 ORF from 203 bp to 3073 bp; peptide length: 957 Category: similarity to known protein 1 MAHYITFLCM VLVLLLQNSV LAEDGEVRSS CRTAPTDLVF ILDGSYSVGP 51 ENFEIVKKWL VNITKNFDIG PKFIQVGVVQ YSDYPVLEIP LGSYDSGEHL 101 TAAVESILYL GGNTKTGKAI QFALDYLFDK SSRFLTKIAV VLTDGKSQDD 151 VKDAAQAARD SKITLFAIGV GSETEDAELR AIANKPSSTY VFYVEDYIAI 201 SKIREVMKQK LCEESVCPTR IPVAARDERG FDILLGLDVN KKVKKRIQLS 251 PKKIKGYEVT SKVDLSELTS NVFPEGLPPS YVFVSTQRFK VKKIWDLWRI 301 LTIDGRPQIA VTLNGVDKIL LFTTTSVING SQVVTFANPQ VKTLFDEGWH 351 QIRLLVTEQD VTLYIDDQQI ENKPLHPVLG ILINGQTQIG KYSGKEETVQ 401 FDVQKLRIYC DPEQNNRETA CEIPGFNGEC LNGPSDVGST PAPCICPPGK 451 PGLQGPKGDP GLPGNPGYPG QPGQDGKPGY QGIAGTPGVP GSPGIQGARG 501 LPGYKGEPGR DGDKGDRGLP GFPGLHGMPG SKGEMGAKGD KGSPGFYGKK 551 GAKGEKGNAG FPGLPGPAGE PGRHGKDGLM GSPGFKGEAG SPGAPGQDGT 601 RGEPGIPGFP GNRGLMGQKG EIGPPGQQGK KGAPGMPGLM GSNGSPGQPG 651 TPGSKGSKGE PGIQGMPGAS GLKGEPGATG SPGEPGYMGL PGIQGKKGDK 701 GNQGEKGIQG QKGENGRQGI PGQQGIQGHH GAKGERGEKG EPGVRGAIGS 751 KGESGVDGLM GPAGPKGQPG DPGPQGPPGL DGKPGREFSE QFIRQVCTDV 801 IRAQLPVLLQ SGRIRNCDHC LSQHGSPGIP GPPGPIGPEG PRGLPGLPGR 851 DGVPGLVGVP GRPGVRGLKG LPGRNGEKGS QGFGYPGEQG PPGPPGPEGP 901 PGISKEGPPG DPGLPGKDGD HGKPGIQGQP GPPGICDPSL CFSVIARRDP 951 FRKGPNY BLASTP hits Entry HSC0L7A1X_1 from database TREMBL: gene: "COL7A1"; product: "collagen type VII"; Homo sapiens (clones: CW52-2, CW27-6, CW15-2, CW26-5, 11-67) collagen type VII intergenic region and (COL7A1) gene, complete eds. Score = 949, P = 3.4e-122, identities = 237/553, positives = 281/553 Entry CA17_HUMAN from database SWISSPROT: COLLAGEN ALPHA l(VII) CHAIN PRECURSOR (LONG-CHAIN COLLAGEN) (LC COLLAGEN). >TREMBL: HSCOL7Al_l gene: "COL7A1"; product: "alpha-1 type VII collagen"; Human alpha-1 type VII collagen (COL7A1) mRNA, complete eds . Score = 949, P = 3.6e-122, identities = 237/553, positives = 281/553 Alert BLASTP hits for DKFZphfbr2_2b5, frame 2 No Alert BLASTP hits found Pedant information for DKFZphfbr2_2b5, frame 2 Report for DKFZphfbr2_2b5.2 [LENGTH] 957 [MW] 99413.38 [pi] 8.49 [HOMOL] PIR:A40020 collagen alpha l(XII) chain precursor - chicken 9e-90 [BLOCKS] BL01119B Copper-fist domain proteins [BLOCKS] BL00313B [BLOCKS] BL01113A Clq domain proteins [BLOCKS] BL00420A Speract receptor repeat proteins domain proteins [SCOP] dlzoob_ 3.45.1.1.1 Integrin CDlla/CD18 (LFA-1) [Human (Horn 2e-58 [SCOP] dlido 3.45.1.1.2 Integrin CR3 (CDllb/CD18) , alpha subunit [Huma 8e-62 [EC] 3.1.1.7 Acetylcholmesterase 7e-24 [PIRKW] blocked amino end le-43 [PIRKW] duplication 7e-46 [PIRKW] cornea le-35 [PIRKW] lung 2e-40 [PIRKW] leukocyte le-42 [PIRKW] skin le-40 [PIRKW] transmembrane protein le-37 [PIRKW] cartilage 3e-59 [PIRKW] hydroxylysine 4e-62 [PIRKW] connective tissue 3e-43 [PIRKW] triple helix 5e-82 [PIRKW] homotπmer 2e-37 [PIRKW] bone 6e-40 [PIRKW] Alport syndrome le-42 [PIRKW] lammin binding 2e-40 [PIRKW] liver 2e-40 [PIRKW] glycoprotein 5e-82 [PIRKW] carboxylic ester hydrolase 7e-24 [PIRKW] disulfide bond 7e-46 [PIRKW] cell binding 7e-46 [PIRKW] heterotrimer 4e-62 [PIRKW] calcium binding 8e-28 [PIRKW] alternative splicing 5e-82 [PIRKW] coiled coil 5e-82 [PIRKW] basement membrane 7e-46 [PIRKW] trimer 5e-82 [PIRKW] pyroglutamic acid 3e-43 [PIRKW] hydroxyprolme 4e-62 [PIRKW] extracellular matrix 5e-82 [PIRKW] chondroit sulfate proteoglycan 6e-41 [PIRKW] sulfoprotein 7e-39 [PIRKW] kidney le-42 [PIRKW] angiogenesis inhibitor 6e-36 [PIRKW] Ehlers-Danlos syndrome 2e-40 [SUPFAM] fibronect type III repeat homology 5e-82 [SUPFAM] scavenger receptor cyste e-rich domain homology le-37 [SUPFAM] C-type lectin homology 6e-30 [SUPFAM] collagen alpha 2(1) chain 5e-40 [SUPFAM] collagen alpha 1(1) chain 6e-44 [SUPFAM] fibrillar collagen carboxyl-terminal homology 6e-44 [SUPFAM] animal Kumtz-type proteinase inhibitor homology 2e-3£ [SUPFAM] fibronect n type II repeat homology 6e-21 [SUPFAM] complement Clq carboxyl-terminal homology le-38 [SUPFAM] collagen alpha 3 (VI) chain 2e-31 [SUPFAM] collagen alpha 1(IV) chain 7e-46 [SUPFAM] collagen alpha 1(VI) chain 2e-37 [SUPFAM] von Willebrand factor type C repeat homology 6e-44 [SUPFAM] unassigned collagens 4e-62 [SUPFAM] von Willebrand factor type A repeat homology 5e-82 [SUPFAM] collagen alpha 1 (XIV) chain 5e-82 [SUPFAM] pulmonary surfactant protein D 6e-30 [SUPFAM] collagen alpha 1 (V) chain 7e-39 [SUPFAM] collagen alpha l(VIII) chain le-38 [SUPFAM] EGF homology le-35 [PROSITE] AMIDATION 3 [PROSITE] MYRISTYL 14 [PROSITE] CK2_PHOSPHO_SITE 13 [PROSITE] PKC_PHOSPHO_SI E 8 [PROSITE] ASN_GLYCOSYLATION 2 [PFAM] von Willebrand factor type A domain [KW] Irregular [KW] 3D [KW] SIGNAL_PEPTIDE 23 [KW] LOW COMPLEXITY 24.24 % SEQ MAHYITFLCMVLVLLLQNSVLAEDGEVRSSCRTAPTDLVFILDGSYSVGPENFEIVKKWL SEG latzB CCCEEEEEEEECCCCCCHHHHHHHHHHH SEQ VNITKNFDIGPKFIQVGVVQYSDYPVLEIPLGSYDSGEHLTAAVESILYLGGNTKTGKAI SEG latzB HHHHHHCCBTTTTEEEEEEEETTTEEEEETTTTTTTHHHHHHHHHHCCCCCCCCCHHHHH SEQ QFALDYLFDKSSRFLTKIAVVLTDGKSQDDVKDAAQAARDSKITLFAIGVGSETEDAELR SEG latzB HHHHHHHHCCTTTTTEEEEEEEECCCTTTTHHHHHHHHHHHCEEEEEEEECCCCCHHHHH SEQ AIANKPSSTYVFYVEDYIAISKIREVMKQKLCEESVCPTRIPVAARDERGFDILLGLDVN SEG latzB HHHGGGGGGGCECCHHHHHHHHHCHHHHHHHH SEQ KKVKKRIQLSPKKIKGYEVTSKVDLSELTSNVFPEGLPPSYVFVSTQRFKVKKIWDLWRI SEG latzB SEQ LTIDGRPQIAVTLNGVDKILLFTTTSVINGSQVVTFANPQVKTLFDEGWHQIRLLVTEQD SEG latzB SEQ VTLYIDDQQIENKPLHPVLGILINGQTQIGKYSGKEETVQFDVQKLRIYCDPEQNNRETA SEG latzB SEQ CEI PGFNGECLNGPSDVGSTPAPCICPPGKPGLQGPKGDPGLPGNPGYPGQPGQDGKPGY SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx latzB SEQ QGIAGTPGVPGSPGIQGARGLPGYKGEPGRDGDKGDRGLPGFPGLHGMPGSKGEMGAKGD SEG xx latzB SEQ KGSPGFYGKKGAKGEKGNAGFPGLPGPAGEPGRHGKDGLMGSPGFKGEAGSPGAPGQDGT SEG xxxxxxxxxxxxx latzB SEQ RGEPGIPGFPGNRGLMGQKGEIGPPGQQGKKGAPGMPGLMGSNGSPGQPGTPGSKGSKGE SEG xxxxxxxxxxxxxxxxxxxxxx latzB SEQ PGIQGMPGASGLKGEPGATGSPGEPGYMGLPGIQGKKGDKGNQGEKGIQGQKGENGRQGI SEG xxxxxxxxxxxxxxxxxxxxx latzB SEQ PGQQGIQGHHGAKGERGEKGEPGVRGAIGSKGESGVDGLMGPAGPKGQPGDPGPQGPPGL SEG xxxxxxxxxxx xxxxxxxxxxxxxxxxxxxx latzB SEQ DGKPGREFSEQFIRQVCTDVIRAQLPVLLQSGRIRNCDHCLSQHGSPGIPGPPGPIGPEG SEG xxxxx xxxxxxxxxxxxxxxx latzB SEQ PRGLPGLPGRDGVPGLVGVPGRPGVRGLKGLPGRNGEKGSQGFGYPGEQGPPGPPGPEGP SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxx latzB SEQ PGISKEGPPGDPGLPGKDGDHGKPGIQGQPGPPGICDPSLCFSVIARRDPFRKGPNY SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx latzB Prosite for DKFZphfbr2_2b5.2 PS00001 62->66 ASN_GLYCOΞYLATION PDOC00001 PS00001 329->333 ASN_GLYCOSYLATION PDOC00001 PS00005 30->33 PKC_PHOSPHO_SITE PDOC00005 PS00005 116->119 PKC_PHOSPHO_SITE PDOC00005 PS00005 131->134 PKC_PHOSPHO_SITE PDOC00005 PS00005 250->253 PKC_PHOSPHO_SITE PDOC00005 PS00005 260->263 PKC_PHOSPHO_SITE PDOC00005 PS00005 286->289 PKC_PHOSPHO_SITE PDOC00005 PS00005 393->396 PKC_PHOSPHO_SITE PDOC00005 PS00005 811->814 PKC_PHOSPHO_SITE PDOC00005 PS00006 147->151 CK2_PHOSPHO_SITE PDOC00006 PS00006 172->176 CK2_PHOSPHO_SITE PDOC00006 PS00006 261->265 CK2_PHOSPHO_SITE PDOC00006 PS00006 343->347 CK2_PHOSPHO_SITE PDOC00006 PS00006 357->361 CK2_PHOSPHO_SITE PDOC00006 PS00006 393->397 CK2_PHOSPHO_ΞITE PDOC00006 PS00006 419->423 CK2_PHOSPHO_SITE PDOC00006 PS00006 531->535 CK2_PHOSPHO_SITE PDOC00006 PS00006 600->604 CK2_PHOSPHO_SITE PDOC00006 PS00006 657->661 CK2_PHOΞPHO_SITE PDOC00006 PS00006 681->685 CK2_PHOSPHO_SITE PDOC00006 PS00006 750->754 CK2_PHOSPHO_SITE PDOC00006 PS00006 754->758 CK2_PHOSPHO_SITE PDOC00006 PS00008 92->98 MYRISTYL PDOC00008 PS00008 112->118 MYRISTYL PDOC00008 PS00008 236->242 MYRISTYL PDOC00008 PS00008 276->282 MYRISTYL PDOC00008 PS00008 380->386 MYRISTYL PDOC00008 PS00008 494->500 MYRISTYL PDOC00008 PS00008 527->533 MYRISTYL PDOC00008 PS00008 596->602 MYRISTYL PDOC00008 PS00008 638->644 MYRISTYL PDOC00008 PS00008 650->656 MYRISTYL PDOC00008 PS00008 653->659 MYRISTYL PDOC00008 PS00008 665->671 MYRISTYL PDOC00008 PS00008 743->749 MYRISTYL PDOC00008 PS00008 746->752 MYRISTYL PDOC00008 PS00009 547->551 AMIDATION PDOC00009 PS00009 628->632 AMIDATION PDOC00009 PS00009 694->698 AMIDATION PDOC00009 Pfam for DKFZphfbr2_2b5.2 HMM_NAME von Willebrand factor type A domain HMM *DIVFLIDGSdSIGpqNFNrMKDFIeRMMERMDIgPDwIRVGVVQYSdNP D+VF++DGS S+GP NF+++K+ ++++ ++DIGP+ I+VGVVQYSD P Query 37 DLVFILDGSYSVGPENFEIVKKWLVNITKNFDIGPKFIQVGVVQYSDYP 85 HMM RqEmrFmFNDYQNKeEILQalqqMMyWMgggTNTGeAIQYVvrNMFweer E +++ Y + E++++A+ ++ ++GG T+TG AIQ++++++F +++ Query 86 VLE—IPLGSYDSGEHLTAAVESIL-YLGGNTKTGKAIQFALDYLFDKSS 132 HMM GmRWenvPQVMIIITDGRSQDDIRDpIneMrrmaGIqvFalGIGNhDNnn + +++++++TDG+SQDD++D+++++R+ 1+ FAIG+G Query 133 RF LTKIAVVLTDGKSQDDVKDAAQAARD-SKITLFAIGVGSETE— 175 HMM WeELRelASePdEdHVFyVdDFeeLdnMqeqL* +ELR IA++P++ +VFYV+D+ +++ ++E + Query 176 DAELRAIANKPSSTYVFYVEDYIAISKIREVM 207 DKFZphfbr2_2cl group: brain derived DKFZphfbr2_2cl encodes a novel 697 amino acid protein without similarity to known proteins. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of brain-specific genes . unknown complete cDNA, complete eds, EST hits Sequenced by Qiagen Locus : unknown Insert length: 3973 bp Poly A stretch at pos. 3914, polyadenylation signal at pos. 3900 1 GGGGGGATTT CGGCGGCGGA AACATGGCGG TCGCGGCCGG GCCGGTAACG 51 GAGAAAGTTT ACGCCGACAC TGGCCTGTAT TAGCGCGTAT GGCCTCGGGC 101 CCTCGTTCCC CAAGGCGTGC CGCCTCCCTG TTCTCAGTCG CAGGCTGAAG 151 CCTTGTCTGC TCTCCTCCTT TTTGGTTTGG TTTTGGAACT GACTCCGAGG 201 GTTGGGAGAG CGCGTTGGTG GCGACGGCCG AGTCAGATCA CTATAAACAA 251 AATTTCCACA AGAGAAAATG TTGAAATAGG AGTTGCGGAT ACATTGGATA 301 TACTGGATGA AATACAAGCG GTTAATTTTT GTAACGTGAG GGAAAAGCCC 351 ACATTGCTGG TTACATGTGT AAATCACTGC GTTATTGCTT TAGTCATTGT 401 CTCTATTTAG CAATGACAAG ACTGGAAGAA GTAAATAGAG AAGTGAACAT 451 GCATTCTTCA GTGCGGTATC TTGGCTATTT AGCCAGAATC AATTTATTGG 501 TTGCTATATG CTTAGGTCTA TACGTAAGAT GGGAAAAAAC AGCAAATTCC 551 TTAATTTTGG TAATTTTTAT TCTTGGTCTT TTTGTTCTTG GAATCGCCAG 601 CATACTCTAT TACTATTTTT CAATGGAAGC AGCAAGTTTA AGTCTCTCCA 651 ATCTTTGGTT TGGATTCTTG CTTGGCCTCC TATGTTTTCT TGATAATTCA 701 TCCTTTAAAA ATGATGTAAA AGAAGAATCA ACCAAATATT TGCTTCTAAC 751 ATCCATAGTG TTAAGGATAT TGTGCTCTCT GGTGGAGAGA ATTTCTGGCT 801 ATGTCCGTCA TCGGCCCACT TTACTAACCA CAGTTGAATT TCTGGAGCTT 851 GTTGGATTTG CCATTGCCAG CACAACTATG TTGGTGGAGA AGTCTCTGAG 901 TGTCATTTTG CTTGTTGTAG CTCTGGCTAT GCTGATTATT GATCTGAGAA 951 TGAAATCTTT CTTAGCTATT CCAAACTTAG TTATTTTTGC AGTTTTGTTA 1001 TTTTTTTCCT CATTGGAAAC TCCCAAAAAT CCGATTGCTT TTGCGTGTTT 1051 TTTTATTTGC CTGATAACTG ATCCTTTCCT TGACATTTAT TTTAGTGGAC 1101 TTTCAGTAAC TGAAAGATGG AAACCCTTTT TGTACCGTGG AAGAATTTGC 1151 AGAAGACTTT CAGTCGTTTT TGCTGGAATG ATTGAGCTTA CATTTTTTAT 1201 TCTTTCCGCA TTCAAACTTA GAGACACTCA CCTCTGGTAT TTTGTAATAC 1251 CTGGCTTTTC CATTTTTGGA ATTTTCAGGA TGATTTGTCA TATTATTTTT 1301 CTTTTAACTC TTTGGGGATT CCATACCAAA TTAAATGACT GCCATAAAGT 1351 ATATTTTACT CACAGGACAG ATTACAATAG CCTTGATAGA ATCATGGCAT 1401 CCAAAGGGAT GCGCCATTTT TGCTTGATTT CAGAGCAGTT GGTGTTCTTT 1451 AGTCTTCTTG CAACAGCGAT TTTGGGAGCA GTTTCCTGGC AGCCAACAAA 1501 TGGAATTTTC TTGAGCATGT TCCTAATCGT TTTGCCATTG GAATCCATGG 1551 CTCATGGGCT CTTCCATGAA TTGGGTAACT GTTTAGGAGG AACATCTGTT 1601 GGATATGCTA TTGTGATTCC CACCAACTTC TGCAGTCCTG ATGGTCAGCC 1651 AACACTGCTT CCCCCAGAAC ATGTACAGGA GTTAAATTTG AGGTCTACTG 1701 GCATGCTCAA TGCTATCCAA AGATTTTTTG CATATCATAT GATTGAGACC 1751 TATGGATGTG ACTATTCCAC AAGTGGACTG TCATTTGATA CTCTGCATTC 1801 CAAACTAAAA GCTTTCCTCG AACTTCGGAC AGTGGATGGA CCCAGACATG 1851 ATACGTATAT TTTGTATTAC AGTGGGCACA CCCATGGTAC AGGAGAGTGG 1901 GCTCTAGCAG GTGGAGATAC ACTACGCCTT GACACACTTA TAGAATGGTG 1951 GAGAGAAAAG AATGGTTCCT TTTGTTCCCG GCTTATTATC GTATTAGACA 2001 GCGAAAATTC AACCCCTTGG GTGAAAGAAG TGAGGAAAAT TAATGACCAG 2051 TATATTGCAG TGCAAGGAGC AGAGTTGATA AAAACAGTAG ATATTGAAGA 2101 AGCTGACCCG CCACAGCTAG GTGACTTTAC AAAAGACTGG GTAGAATATA 2151 ACTGCAACTC CTGTAATAAC ATCTGCTGGA CTGAAAAGGG ACGCACAGTG 2201 AAAGCAGTAT ATGGTGTGTC AAAACGGTGG AGTGACTACA CTCTGCATTT 2251 GCCAACGGGA AGCGATGTGG CCAAGCACTG GATGTTACAC TTTCCTCGTA 2301 TTACATATCC CCTAGTGCAT TTGGCAAATT GGTTATGCGG TCTGAACCTT 2351 TTTTGGATCT GCAAAACTTG TTTTAGGTGC TTGAAAAGAT TAAAAATGAG 2401 TTGGTTTCTT CCTACTGTGC TGGACACAGG ACAAGGCTTC AAACTTGTCA 2451 AATCTTAATT TGGACCCCAA AGCGGGATAT TAATAAGCAC TCATACTACC 2501 AATTATCACT AACTTGCCAT TTTTTGTATG CTGTATTTTT ATTTGTGGAA 2551 AATACCTTGC TACTTCTGTA GCTGCTCTCA CTTTGTCTTT TCTTAAGTAA 2601 TTATGGTATA TATAAGGCGT TGGGAAAAAA CATTTTATAA TGAAAGTATG 2651 TAGGGAGTCA AATGCTTACT GTAAATGCAT AAGAGACGTT AAAAATAACA 2701 CTGCACTTTC AGGAATGTTT GCTTATGGTC CTGATTAGAA AGAAACAGTT 2751 GTCTATGCTC TGCAATGGTC AATGATGAAT TACTAATGCC TTATTTTCTA 2801 GGCATATAAT AATAGTTTAG AGAATGTAGA CCAGATAAAT TTGTTTACTG 2851 TTTTAAGAAA ACTACCAGTT TACTTACAGA AGATTCTTTT TTCCAAACAG 2901 TAGGTTTCAT CCAAGACCAT TTGAAGAACT GCAAACTCTT TCTCTTAGAA 2951 AAGAAAGAGG GCAGCCTAAA ATAAACGCAA AATTTGCTTA TACTCCATCA 3001 CATTCAGATG TCTTGGTTGT GACTTATTAC CAGTGTGGCA GAGAACCCAA 3051 GTTACATTTT AGATCAAAAT ATTCTTTATG TAGGTATTGT TAAAAGGCTA 3101 GAGCCTACAA GTTGCTCTTC CATGCGTTGG TCAGGGGGCC CTGAAAACAC 3151 TGGTAATATT AAGAGTCTTT CTCAGGGTAA CTTAATGTTT TCTTAATGAA 3201 CAGTGTTTCC AGCTACAAAT TCTTCCAATA AATTGTCTTC CTTTTTGAAA 3251 AGTACTCTCA TAGAAGAAAT TTAGCAATTT CTCGTTGACT GACTCAGTCT 3301 ATTTTAAGTA TTCAGAAAAG ATTTTGATCC CCATTGAGTT AATGCTCTGC 3351 CTTGAAAATT ATTTTTCTGA TCCTTGTTAG TGATAACATT TTTTTTCTAC 3401 TGAAGGTCAG AGGATAGGAA ACAAGTATTT CTCTTCTGGT ATACATGTAA 3451 TGTATTCTGT AAAAAAGTAT TCATATTGGC AATTTTAGTT AGGCATAATA 3501 TTGTGGTTGT AATTTTTAAA ACTTAGTGTT TTGTCTGATT AAAGCAGGCA 3551 CTGATCAGGG TATCTCCTAA GAGGTAATTC ACTTCTTATT CCTTTCCAAT 3601 AATTATTACA TTCTAAATTT TCATCTATGA GAAATAACAA ACAAGAAGGG 3651 AATAGAATTA AATTGGGGTA TAATCTAATC TTCATTGTTT AAATGGTTTG 3701 CCTTCTCACC ATTGAAGCCA TTTTTTTATA GCCTCAGAAA GAGGAAATAA 3751 TGCCTCCACC ATTTTCTACC TGGTGACTTG AAAATTGAAC TTTTAAGTTA 3801 GGAAGAAGTT AGAGTCAGGG AACTTGTATA CCACTATCTA TGCAGCATTG 3851 TTATAGTCTG ATTATTTCTG TGTTTTGAAT ATGATTTTCC TAATGCTCTA 3901 AATAAAATTT TGTTAAAAAT CAAAAAAAAA AAAAAAAAAA CTTATCGATA 3951 CCGTCGACCT CGATGATGTC GAC BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 2 ORF from 365 bp to 2455 bp; peptide length: 697 Category: putative protein Classification: unset 1 MCKSLRYCFS HCLYLAMTRL EEVNREVNMH SSVRYLGYLA RINLLVAICL 51 GLYVRWEKTA NSLILVIFIL GLFVLGIASI LYYYFSMEAA ΞLSLSNLWFG 101 FLLGLLCFLD NSSFKNDVKE ESTKYLLLTS IVLRILCSLV ERISGYVRHR 151 PTLLTTVEFL ELVGFAIAST TMLVEKSLSV ILLVVALAML IIDLRMKSFL 201 AIPNLVIFAV LLFFSSLETP KNPIAFACFF ICLITDPFLD IYFSGLSVTE 251 RWKPFLYRGR ICRRLSVVFA GMIELTFFIL ΞAFKLRDTHL WYFVIPGFSI 301 FGIFRMICHI IFLLTLWGFH TKLNDCHKVY FTHRTDYNSL DRIMASKGMR 351 HFCLISEQLV FFSLLATAIL GAVSWQPTNG IFLSMFLIVL PLESMAHGLF 401 HELGNCLGGT SVGYAIVIPT NFCSPDGQPT LLPPEHVQEL NLRSTGMLNA 451 IQRFFAYHMI ETYGCDYSTS GLSFDTLHΞK LKAFLELRTV DGPRHDTYIL 501 YYSGHTHGTG EWALAGGDTL RLDTLIEWWR EKNGSFCSRL IIVLDSENST 551 PWVKEVRKIN DQYIAVQGAE LIKTVDIEEA DPPQLGDFTK DWVEYNCNSC 601 NNICWTEKGR TVKAVYGVSK RWSDYTLHLP TGSDVAKHWM LHFPRITYPL 651 VHLANWLCGL NLFWICKTCF RCLKRLKMSW FLPTVLDTGQ GFKLVKS BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_2cl, frame 2 PIR:A71148 hypothetical protein PH0395 - Pyrococcus horikoshn, N = 1, Score = 96, P = 0.12 >PIR:A71148 hypothetical protein PH0395 Pyrococcus horikoshn Length = 288 HSPs: Score = 96 (14.4 bits), Expect = 1.3e-01, P = 1.2e-01 Identities = 59/234 (25%), Positives = 116/234 (49%) Query: 77 IASILYYYFSMEAASLSLSNLWFGFLL--GL—LCFLDNSSFKNDVKEESTKYLLLTΞIV 132 ++ +LYY F+ A ++ L G+LL + L +L N + V+ + K + ++ Sbjct: 57 LSLVLYYLFAFSALK-TIIFLALGYLLMNSIYELGYLMNDTISRRVEGKVHKVRVKLTVF 115 Query: 133 LRILCSLVERISGYVRHRPTLLTTVEFLELVGFAIASTTMLVEKSLSVILLVVALAMLII 192 +L +L I YV ++ T+ FL+LVG ++ +L E +L ++ L+ L + Sbjct: 116 DSLLIALSRAI--YV VIFTLVFLKLVGLQYSTQVILAEVTLFLVFLLYDLTPKHV 168 Query: 193 DLRMKSFLAIPNLVIFAVLLFFSSLET-PKNPIAFACFFICLITDPFLDIYFSGLSVTER 251 M SF + + F +LL F T +N I + FI I F ++ + + Sbjct: 169 RTVMLSF-PLKFMKAFVLLLPFIITGTLVENVITLΞ—FILPIAVRFSQAHYLKTACKDN 225 Query: 252 WKPFLYRGRICRRLSVVFAGMIEL-TFFILSAFK-LRDTHLW-YFVIPGFSIFGIFRMIC 308 P ++ R+ R S+++ + L TF +L +F L +T L ++IP F++ + ++ Sbjct: 226 -PPRDFKRRV-ERFSMMYLQVTSLSTFTVLVSFVYLGNTDLLRQYLIP-FAVNVVLILLΞ 282 Query: 309 HI 310 ++ Sbjct: 283 YL 284 Pedant information for DKFZphfbr2_2cl, frame 2 Report for DKFZphfbr2_2cl .2 [LENGTH] 697 [MW] 79741.46 [pl] 8.41 [KW] TRANSMEMBRANE 11 [KW] LOW COMPLEXITY 9.76 % SEQ MCKSLRYCFSHCLYLAMTRLEEVNREVNMHΞΞVRYLGYLARINLLVAICLGLYVRWEKTA SEG PRD ccceeehhhhhhhhhhhhhhhhhhhhhhccceeeehhhhhhhhhhhhhhhhhhhcccccc MEM MMMMMMMMMMMMMMMMM SEQ NSLILVIFILGLFVLGIASILYYYFSMEAASLSLSNLWFGFLLGLLCFLDNSSFKNDVKE SEG ..xxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxx PRD ccceeeeccccchhhhhchhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccccccccc MEM ...MMMMMMMMMMMMMMMMM MMMMMMMMMMMMMMMMM SEQ ESTKYLLLTSIVLRILCSLVERISGYVRHRPTLLTTVEFLELVGFAIASTTMLVEKSLSV SEG xxxxxxxxxxxx xxxx PRD ccchhhhhhhhhhhhhhhhhhhceeeeccccccchhhhhhhhhhhhhhhhhhhhhhhhhh MEM ....MMMMMMMMMMMMMMMMM MMM SEQ ILLVVALAMLIIDLRMKSFLAIPNLVIFAVLLFFSSLETPKNPIAFACFFICLITDPFLD SEG xxxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhhhcccchhhhhhhhhhcccccccccchhhhhhhhhcccccee MEM MMMMMMMMMMMMMM ...MMMMMMMMMMMMMMMMM MMMMMMMMMMMMMMMMM. SEQ IYFSGLSVTERWKPFLYRGRICRRLSVVFAGMIELTFFILSAFKLRDTHLWYFVIPGFSI SEG PRD eeeccccccccccceeecccccccchhhhhhhhhhhhhhhhhhhccccceeeeeeccccc MEM MMMMMMMMMMMMMMMMM M SEQ FGIFRMICHIIFLLTLWGFHTKLNDCHKVYFTHRTDYNSLDRIMASKGMRHFCLISEQLV SEG PRD hhhhhhhhhhhhhhhhhcccccccceeeeeeeccccccchhhhhhhcccchhhhhhhhhh MEM MMMMMMMMMMMMMMMM MM SEQ FFSLLATAILGAVSWQPTNGIFLSMFLIVLPLESMAHGLFHELGNCLGGTSVGYAIVIPT SEG PRD hhhhhhhhhhhhcccccccchhhhhhhheeehhhhhhhhhhccccccccccceeeeeeec MEM MMMMMMMMMMMMMMM ....MMMMMMMMMMMMMMMMM SEQ NFCSPDGQPTLLPPEHVQELNLRSTGMLNAIQRFFAYHMIETYGCDYSTSGLSFDTLHSK SEG PRD ccccccccccccccccccccccccchhhhhhhhhhhhhhhhccccccccccccchhhhhh MEM SEQ LKAFLELRTVDGPRHDTYILYYSGHTHGTGEWALAGGDTLRLDTLIEWWREKNGSFCSRL SEG PRD hhhhhhhhhccccccceeeeeeccccccccceeeccccchhhhhhhhhhhhccccceeee MEM SEQ IIVLDSENSTPWVKEVRKINDQYIAVQGAELIKTVDIEEADPPQLGDFTKDWVEYNCNSC SEG PRD eeeeecccccccchhhhhhccceeeeccceeeeeeeecccccccccccccceeeeccccc MEM SEQ NNICWTEKGRTVKAVYGVSKRWSDYTLHLPTGSDVAKHWMLHFPRITYPLVHLANWLCGL SEG PRD cceeeecccceeeeeeeecccccceeeecccccchhhhhhhcccccccchhhhhhhhhcc MEM SEQ NLFWICKTCFRCLKRLKMSWFLPTVLDTGQGFKLVKS SEG PRD eeeeeehhhhhhhhhhhhhhcceeeeccccccccccc MEM (No Prosite data available for DKFZphfbr2_2cl .2) (No Pfam data available for DKFZphfbr2_2cl .2) DKFZphfbr2 2cl7 group: signal transduction DKFZphfbr2_ 2 cl7.3 encodes a novel 446 amino ac d protein with similarity to yeast YMR131C and mammalian retinoblastoma-binding protein RbAp46 The protein contains 1 WD-40 repeat, which is typical for the beta-transducm subunit of G- proteins . The beta subunits seem to be required for the replacement of GDP by GTP as well as for membrane anchoring and receptor recognition. The new protein can find application m modulating/blocking G-protein-dependent pathways. similarity to YMR131C and retinoblastoma-bmding protein RbAp46 complete cDNA, complete eds, EST hits Sequenced by Qiagen Locus: unknown Insert length: 2248 bp Poly A stretch at pos. 2230, polyadenylation signal at pos. 2200 1 TGGGGAAGAT GGCGGCGCGC AAGGGTCGGC GTCGCACGTG TGAAACCGGG 51 GAACCCATGG AAGCCGAGTC CGGCGACACA AGTTCCGAGG GCCCGGCCCA 101 GGTCTACCTG CCCGGCCGGG GGCCGCCGCT ACGCGAAGGG GAGGAGCTGG 151 TCATGGACGA GGAGGCCTAT GTGCTCTACC ACCGAGCGCA GACTGGCGCC 201 CCCTGTCTCA GCTTTGACAT AGTCCGGGAT CACCTGGGAG ACAACCGGAC 251 AGAGCTTCCT CTTACACTTT ACTTGTGTGC TGGGACCCAG GCTGAGAGCG 301 CCCAGAGCAA CAGACTGATG ATGCTTCGGA TGCACAATCT GCATGGGACA 351 AAGCCCCCAC CCTCAGAGGG CAGTGATGAA GAAGAAGAGG AGGAAGATGA 401 AGAGGATGAA GAAGAGCGGA AACCTCAGCT GGAGCTGGCC ATGGTGCCCC 451 ACTATGGTGG CATCAACCGA GTTCGGGTGT CATGGCTGGG TGAAGAGCCT 501 GTGGCTGGGG TGTGGTCAGA GAAGGGCCAG GTGGAGGTGT TTGCGCTGCG 551 GCGGCTTCTG CAGGTGGTGG AGGAGCCCCA GGCCCTGGCA GCCTTCCTCC 601 GGGATGAGCA GGCCCAAATG AAGCCCATCT TCTCCTTCGC TGGACACATG 651 GGCGAGGGCT TTGCCCTTGA CTGGTCCCCC CGGGTGACCG GTCGCCTGCT 701 GACCGGTGAC TGTCAAAAGA ACATCCACCT CTGGACACCT ACGGACGGCG 751 GCTCCTGGCA CGTGGACCAG CGGCCATTCG TGGGCCACAC ACGCTCTGTG 801 GAGGACCTGC AGTGGTCACC GACTGAGAAC ACGGTGTTTG CCTCCTGCTC 851 AGCTGACGCC TCCATCCGCA TCTGGGACAT CCGGGCAGCC CCCAGCAAGG 901 CCTGCATGCT CACCACAGTC ACCGCCCATG ATGGGGACGT CAATGTCATC 951 AGCTGGAGCC GCCGGGAGCC CTTCCTGCTC AGTGGCGGGG ATGATGGGGC 1001 CCTCAAGATC TGGGACCTTC GGCAGTTCAA GTCTGGTTCC CCAGTGGCCA 1051 CCTTCAAGCA GCACGTGGCC CCCGTGACCT CCGTCGAGTG GCACCCCCAG 1101 GACAGCGGGG TCTTTGCAGC CTCGGGTGCA GACCACCAGA TCACACAGTG 1151 GGACCTGGCA GTGGAGCGGG ACCCTGAGGC GGGCGACGTG GAGGCCGACC 1201 CCGGACTGGC CGACCTCCCG CAGCAGCTGC TGTTCGTGCA CCAGGGCGAG 1251 ACCGAGCTGA AGGAGCTGCA CTGGCACCCG CAGTGCCCAG GGCTCCTGGT 1301 CAGCACGGCG CTGTCAGGCT TCACCATCTT CCGCACCATC AGCGTCTGAG 1351 GCGTCCCACT GGCTCTGATC TTGCTTCCTG CTTGGAAACT GAAGTCGAAT 1401 TGGGCTCCCC TGGAAGGGGT TCATTCAGGT CTGTTGACTG AGACTGGCCG 1451 GCCTGTGGGC TGCCGTGATG GATTCTGTTT GACGTATTGT TCTCTAGAAG 1501 GCCTGGCTCT GATCCAGTGA CCCCTCTCAC CAAAGAACTC GGTTTAACCA 1551 GGGCTCTGTA AGACCACTCC CACCCAGAGA CTTGTGTGGC CTGGTGTGGC 1601 CTGTGTGTCG GATTCCTTCC TGTCAGCTGT GACCCATTTG ACCTGTGTCC 1651 CCAGAACCCA GTTTTTTGTT TGTTTGTTTG AGACGGAGTC TTGGTCTGTC 1701 GCCCAGGCTG GAGTGCAGTA GCACGATCTT GGCTCACTGC AACCTCCGCC 1751 TCCTGGGTTA AAGTGATTCT CTCAGCTCAG TCTCCCAGGT AGCTGGGATT 1801 ACAGGCATGT GCCACCACAC CCCGTTAATT TTTGTATTTT TAGTAGAGAC 1851 GGGGTTTCAC CATGTTGGCC AGGCTGGTCT CAAATTCTTG ATCTCAAGTG 1901 ATCTGTCCGC CCCGGCCTCC CAGAGTGCTG GGTTGGGATT ACAGGCGTGA 1951 GCCACCGCGT CCGGCTCAGG ACCCAGTTTT GGCTGCTGGT TCCCAGCAGG 2001 GGACTCGGGG GATATACAGT GGCTGCACCA AATTGGAGGT GTGGGTTCCT 2051 CCAACACAAT TTGCTTCTGC CCGTTGTCTT CCTGCCAGCT GGGTTTGGCC 2101 AGGATTTCTC CGTGTGGGGG CTACATGCGA CCCTCTCCCC TCCTCCCTGA 2151 CTTTAGAGGC TGGTGCTGTG TCGGGAGGAA GGTCAGGGCT CCTGAGCAGC 2201 AATAAAGGAC CAGGAAGAGG CCTGAGGTGG AAAAAAAAAA AAAAAAAA BLAST Results o BLAST result Medlme entries No Medline entry Peptide information for frame 3 ORF from 9 bp to 1346 bp; peptide length: 446 Category: similarity to known protein Classification: unset Prosite motifs: WD REPEATS (323-338) 1 MAARKGRRRT CETGEPMEAE SGDTSSEGPA QVYLPGRGPP LREGEELVMD 51 EEAYVLYHRA QTGAPCLSFD I RDHLGDNR TELPLTLYLC AGTQAESAQS 101 NRLMMLRMHN LHGTKPPPSE GSDEEEEEED EEDEEERKPQ LELAMVPHYG 151 GINRVRVSWL GEEPVAGVWS EKGQVEVFAL RRLLQVVEEP QALAAFLRDE 201 QAQMKPIFSF AGHMGEGFAL DWSPRVTGRL LTGDCQKNIH LWTPTDGGSW 251 HVDQRPFVGH TRSVEDLQWS PTENTVFASC SADASIRIWD IRAAPSKACM 301 LTTVTAHDGD VNVISWSRRE PFLLSGGDDG ALKIWDLRQF KSGSPVATFK 351 QHVAPVTSVE WHPQDSGVFA ASGADHQITQ WDLAVERDPE AGDVEADPGL 401 ADLPQQLLFV HQGETELKEL HWHPQCPGLL VSTALSGFTI FRTISV BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_2cl7, frame 3 TREMBL:AC005917_14 gene: "F3P11.14"; product: "putative WD-40 repeat protein"; Arabidopsis thaliana chromosome II BAC F3P11 genomic sequence, complete sequence., N = 1, Score = 910, P = 2.7e-91 PIR:S53061 hypothetical protein YMR131c - yeast (Saccharomyces cerevisiae), N = 1, Score = 691, P = 4.3e-68 PIR: 149367 retinoblastoma-bindmg protein mRbAp46 - mouse, N = 1, Score = 338, P = l.le-30 PIR: 139181 retinoblastoma-bindmg protein RbAp46 - human, N = 1, Score = 338, P = l.le-30 >TREMBL:AC005917_14 gene: "F3P11.14"; product: "putative WD-40 repeat protein"; Arabidopsis thaliana chromosome II BAC F3P11 genomic sequence, complete sequence. Length = 469 HSPs: Score = 910 (136.5 bits), Expect = 2.7e-91, P = 2.7e-91 Identities = 195/442 (44%), Positives = 259/442 (58%) Query: 18 EAESGDTSSEGPAQVYLPGRGPPLREGEELVMDEEAYVLYHRAQTGAPCLSFDIVRDHLG 77 EA S + S P +V+ PG L +GEEL D AY H G PCLSFDI+ D LG Sbjct: 18 EASSSEIPSI-PTRVWQPGVDT-LEDGEELQCDPSAYNSLHGFHVGWPCLΞFDILGDKLG 75 Query: 78 DNRTELPLTLYLCAGTQAESAQSNRLMMLRMHNLHGTKP PPSEGSDEEEEEEDEED- 133 NRTE P TLY+ AGTQAE A N + + ++ N+ G + P + G+ E+E+E+DE+D Sbjct: 76 LNRTEFPHTLYMVAGTQAEKAAHNSIGLFKITNVSGKRRDVVPKTFGNGEDEDEDDEDDS 135 Query: 134 EEERKPQLELAMVPHYGGINRVRVSWLGEEPVAGVWSEKGQVEVFALRRLLQ 185 E + P +++ V H+G +NR+R + W++ G V+V+ + L Sbjct: 136 DSDDDDGDEASKTPNIQVRRVAHHGCVNRIRAMPQNSH-ICVSWADSGHVQVWDMSSHLN 194 Query: 186 VVEEPQALAAFLRDEQAQMKPIFSFAGHMGEGFALDWSPRVTGRLLTGDCQKNIHLWTPT 245 + E + P+ +F+GH EG+A+DWSP GRLL+GDC+ IHLW P Sbjct: 195 ALAESETEGKDGTSPVLNQAPLVNFSGHKDEGYAIDWSPATAGRLLΞGDCKSMIHLWEPA 254 Query: 246 DGGSWHVDQRPFVGHTRSVEDLQWSPTENTVFASCSADASIRIWDIRAAPSKACMLTTVT 305 G SW VD PF GHT SVEDLQWSP E VFASCS D S+ +WDIR Ξ A + Sbjct: 255 SG-SWAVDPIPFAGHTASVEDLQWSPAEENVFASCSVDGSVAVWDIRLGKSPAL SFK 310 Query: 306 AHDGDVNVISWSRREPFLL-SGGDDGALKIWDLRQFKSGSPV-ATFKQHVAPVTSVEWHP 363 AH+ DVNVISW+R +L SG DDG I DLR K G V A F+ H P+TS+EW Sbjct: 311 AHNADVNVISWNRLASCMLASGSDDGTFSIRDLRLIKGGDAVVAHFEYHKHPITSIEWSA 370 Query: 364 QDSGVFAASGADHQITQWDLAVERDPE AGDVEADPGLADLPQQLLFVHQGETEL 417 ++ A + D+Q+T WDL++E+D E A E DLP QLLFVHQG+ +L Sbjct: 371 HEASTLAVTSGDNQLTIWDLSLEKDEEEEAEFNAQTKELVNTPQDLPPQLLFVHQGQKDL 430 Query: 418 KELHWHPQCPGLLVSTALSGFTIFRTISV 446 KELHWH Q PG+++STA GF I ++ Sbjct: 431 KELHWHNQIPGMIISTAGDGFNILMPYNI 459 Pedant information for DKFZphfbr2_2cl7, frame 3 Report for DKFZphfbr2_2cl7.3 [LENGTH] 446 [MW] 49447.38 [pi] 4.82 [HOMOL] TREMBL:AC005917_14 gene: "F3P11.14"; product: "putative WD-40 repeat protein" Arabidopsis thaliana chromosome II BAC F3P11 genomic sequence, complete sequence, le-90 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YMR131c] 4e-65 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YEL056w] 4e-15 [FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YEL056w] 4e-15 [FUNCAT] 06.07 protein modification (glycolsylation, acylation, myristylation, palmitylation, farnesylation and processing) [S. cerevisiae, YEL056w] 4e-15 [FUNCAT] 04.05.01.07 chromatin modification [S. cerevisiae, YBR195c] 2e-13 [FUNCAT] 10.04.09 regulation of g-protein activity [S. cerevisiae, YBR195c] 2e-13 [FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YBR195C] 2e-13 [ FUNCAT ] 03.16 dna synthesis and replication [S. cerevisiae, YBR195c] 2e-13 [ FUNCAT ] 09.13 biogenesis of chromosome structure [S. cerevisiae, YBR195c] 2e-13 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YPR178w] le-11 [FUNCAT] 04.05.03 mrna processing (splicing) [S. cerevisiae, YPR178w] le-11 [FUNCAT] 06.13 proteolysis [S. cerevisiae, YGL003c] 4e-09 [ FUNCAT ] 03.22 cell cycle control and mitosis [S. cerevisiae, YGL003c] 4e-09 [FUNCAT] 30.09 organization of intracellular transport vesicles [S. cerevisiae, YDL145c] 5e ■ 09 [FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YDL145c] 5e-09 [FUNCAT] 04.05.01.01 general transcription activities [S. cerevisiae, YBR198c TAF90 - TFIID subunit] 6e-09 [FUNCAT] 05.04 translation (initiation, elongation and termination) [S cerevisiae, YMR116C] 5e ■ 08 [FUNCAT] 02.16 fermentation [S. cerevisiae, YMR116c] 5e-08 [FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YLR429w] 3e-07 [FUNCAT] 30.19 peroxisomal organization [S. cerevisiae, YDR142c] 3e-06 [FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YDR142c] 3e-06 [FUNCAT] 08.10 peroxisomal transport [S. cerevisiae, YDR142c] 3e-06 [FUNCAT] 03.13 meiosis [S. cerevisiae, YLR129w] 4e-06 [FUNCAT] 08.01 nuclear transport [S. cerevisiae, YER107c] 4e-06 [FUNCAT] 03.01 cell growth [Ξ. cerevisiae, YKL021C] 4e-06 [FUNCAT] 04.07 rna transport [S. cerevisiae, YER107c] 4e-06 [FUNCAT] 03.25 cytokinesis [Ξ. cerevisiae, YCR057c] 2e-05 (FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YCR057c] 2e-05 [FUNCAT] 01.01.04 regulation of amino-acid metabolism [S. cerevisiae, YIL046w] 2e-05 [FUNCAT] 06.13.01 cytoplasmic degradation [S. cerevisiae, YIL046w] 2e-05 [FUNCAT] 04.01.04 rrna processing [S. cerevisiae, YLLOllw] 3e-05 [FUNCAT] 30.02 organization of plasma membrane [S. cerevisiae, YOR212w] 5e-05 [FUNCAT] 03.07 pheromone response, mating-type determination, sex-specific proteins [S. cerevisiae, YOR212w] 5e-05 [FUNCAT] 10.05.07 g-proteins [S. cerevisiae, YOR212w] 5e-05 [BLOCKS] BL00678 [SCOP] d2trcb_ 2 . 51. 3. 1. 1 Transducin (heterotrimeπc G protein) , gamm 5e-29 [PIRKW] plasma 6e-07 [PIRKW] duplication 4e-12 [PIRKW] hormone 6e-07 [PIRKW] transmembrane protein le-07 [PIRKW] stomach 6e-07 [PIRKW] actin binding le-07 [PIRKW] leucine zipper le-07 [PIRKW] signal transduction 2e-06 [PIRKW] heterotrimer 2e-06 [PIRKW] peripheral membrane protein 6e-07 [PIRKW] GTP binding 2e-06 [SUPFAM] WD repeat homology le-63 [SUPFAM] yeast coatomer complex alpha chain le-07 [SUPFAM] GTP-binding regulatory protein beta chain 4e-07 [SUPFAM] PRL1 protein 8e-09 [ SUPFAM] MSI 1 protein 4e- 12 [ SUPFAM] coatomer complex beta ' chain le-09 [ PROSITE] WD_REPEATS 1 [ PFAM] WD domain , G-beta repeats [ KW] All_Beta [ KW] 3D [KW] LOW COMPLEXITY 3.14 % SEQ MAARKGRRRTCETGEPMEAESGDTSSEGPAQVYLPGRGPPLREGEELVMDEEAYVLYHRA SEG IgotB SEQ QTGAPCLSFDIVRDHLGDNRTELPLTLYLCAGTQAESAQSNRLMMLRMHNLHGTKPPPSE SEG IgotB SEQ GSDEEEEEEDEEDEEERKPQLELAMVPHYGGINRVRVSWLGEEPVAGVWSEKGQVEVFAL SEG .. xxxxxxxxxxxxxx IgotB SEQ RRLLQVVEEPQALAAFLRDEQAQMKPIFSFAGHMGEGFALDWSPRVTGRLLTGDCQKNIH SEG IgotB EEECCCCCEEEEEETTT-TCEEEEEETTTEEE SEQ LWTPTDGGSWHVDQRPFVGHTRSVEDLQWSPTENTVFASCSADASIRIWDIRAAPSKACM SEG IgotB EEETTTT CEEEEEECCCCCEEEEEEETTTCE-EEEEETTTEEEEEETTT—TEEEE SEQ LTTVTAHDGDVNVISWSRREPFLLSGGDDGALKIWDLRQFKSGSPVATFKQHVAPVTSVE SEG IgotB EECBTTBTCCEEEEEETTTTTEEEEEETTTEEEEEE SEQ WHPQDSGVFAASGADHQITQWDLAVERDPEAGDVEADPGLADLPQQLLFVHQGETELKEL SEG IgotB SEQ HWHPQCPGLLVSTALSGFTIFRTISV SEG IgotB Prosite for DKFZphfbr2_2cl7.3 PS00678 323->338 WD REPEATS PDOC00574 Pfam for DKFZphfbr2_2cl7.3 HMM_NAME WD domain, G-beta repeats HMM *MrGHnnWVWCVaFSPDGrWFIvSGSWDgTCRLWD* ++GH+ V ++ +SP + +++S S D ++R+WD Query 257 FVGHTRSVEDLQWSPTENTVFASCSADASIRIWD 290 24.88 304 336 1 34 dkfzphfbr2_2cl7.3 similarity to YMR131c and retinoblastoma- bindmg protein RbAp46 Alignment to HMM consensus: Query *MrGHnnWVWCVaFSPDGrWFIvSGSWDgTCRLWD* + H+++V+ +++S + ++SG++DG +++WD dkfzphfbr2 304 VTAHDGDVNVISWSRREPF-LLΞGGDDGALKIWD 336 DKFZphfbr2_2cl8 group: brain associated DKFZphfbr2_2cl8 encodes a novel 302 ammo acid protein with weak similarity to cyclin- dependent kinase pl30-PITSLRE. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of brain-specific genes . weak similarity to cyclin-dependent kinase pl30-PITΞLRE complete cDNA, complete eds, EST hits Sequenced by Qiagen Locus: unknown Insert length: 2835 bp Poly A stretch at pos. 2817, polyadenylation signal at pos. 2796 1 TGGGGCGGAC GGCGAGGGAG TCCAGAGCCT TGAGCCCGGT GCTCCTCCCT 51 CGCGCAGCGG TGGCTCTGCG GCCGCTGGAG TAAACACTGC CTTTGTTCCC 101 TAGCGCCTCG TCTTTCGTCG CCCCGTGCCC TCACGCCGCC GGGCTCTGGC 151 CGGCCCGCCC TCGGTCCTTG AACCCCATTT CGGCTCGTGC CGTGCGGATG 201 CAGCTGCCGG GCCTGGGTTT GGGCATTGAG CGGGAGGAGG AGGAGGAGCG 251 GCGGCGCCTG GGCGGCATGC GATGGGGAAC TGCTGCTGGA CGCAGTGCTT 301 CGGACTGCTT CGCAAGGAAG CGGGGCGGCT GCAGCGAGTA GGCGGCGGCG 351 GAGGATCCAA GTATTTTAGA ACATGCTCAA GAGGTGAGCA CTTGACAATA 401 GAGTTTGAGA ATCTAGTAGA AAGTGATGAA GGGGAGAGCC CAGGAAGCAG 451 TCATAGGCCT CTTACTGAGG AAGAAATTGT TGACCTAAGA GAAAGGCATT 501 ATGATTCCAT TGCCGAAAAA CAAAAAGATC TTGATGAGAA AATTCAAAAA 551 GAGTTAGCCT TACAAGAAGA GAAGTTAAGA CTAGAAGAAG AAGCTTTATA 601 CGCTGCACAG CGTGAAGCAG CCAGGGCAGC AAAGCAGCGA AAGCTCTTGG 651 AGCAAGAAAG GCAGAGAATT GTGCAGCAAT ATCATCCTTC CAACAATGGA 701 GAATATCAAA GTTCAGGACC AGAAGATGAC TTCGAATCTT GTTTGAGAAA 751 TATGAAGTCA CAGTATGAAG TTTTTCGAAG TAGTAGACTC TCATCAGATG 801 CTACAGTTTT GACACCAAAT ACAGAAAGCA GTTGTGATTT AATGACCAAA 851 ACTAAATCAA CTAGTGGAAA TGACGACAGC ACATCCTTAG ATCTAGAGTG 901 GGAAGATGAA GAAGGAATGA ATAGAATGCT TCCAATGAGA GAACGTTCCA 951 AAACAGAGGA AGACATTCTA CGGGCAGCAC TTAAGTATAG CAACAAGAAG 1001 ACTGGAAGTA ATCCTACATC AGCCTCTGAT GATTCCAATG GGCTGGAGTG 1051 GGAAAATGAT TTTGTTAGTG CCGAAATGGA TGATAATGGA AATTCCGAGT 1101 ATTCTGGATT TGTAAATCCT GTATTAGAAC TGTCTGATTC TGGCATAAGG 1151 CATTCTGACA CAGATCAACA GACTCGATAG GGTAAAATTG TGTGACCTTG 1201 TTTATCAGTT ATGACCAAAT GTTAAAAACC AACTAGAATG TATAAGTGAT 1251 TGTGCTTAGC CTTTTTGTAA GGGAGATGTG TAAGAAACCA TGCTGTAAAT 1301 GCTTATTTTA TTACAAAGGA GTAGGGATGA TAGGATCTGA ATTGATACAG 1351 AATTAAGTGC AATTTCATCA TCTGCCTTCT GCTTTTCAAG ACCAATTTAA 1401 TGGTCCTGTC ATGTTACTGA TTAAATTTAC TTTGTCTTGT CTTTATAGCA 1451 TTTCTGTTTA CTATGGTAGA TTTCCACTTT CAATTTTTAA AATTAATTTT 1501 ACTTTGAATG ATTTATGAAG CCTATTTCAT TGTCTAACTA TGAAAATATT 1551 AAGACTTTTT TGTTAATTCT CAGCCGATGT GAAGGAAGCA TGAGGAGGGA 1601 TCGTCAGACT CAGATTTAGA ATAGTGTTCC CGTTTCCAGC ATTATTTATT 1651 TCTATGACTT CTTTGGATTT TATTATCTAA TAGTAAGTAC AGTTGATGTG 1701 GGTAGATGAC TCTAAGAAAT GCTGAAGTAT CGGCATTACA TGTGTTTATT 1751 TACATGTCCT AGTTTGATAA TGTTGATTCA ATCTGAACAA AAGATAATAT 1801 AAAAATAACC CTTCAGAGTT TGGACATTTC AAGTTGGTAA TAATAAAAAA 1851 TAATATTTAA GAAGATATAT ATATATATAT ATTTAGTTTT TTCCACTTCA 1901 TTTTACATGC CACTATATTG ACTTTAATTG ATATACAGTA TTAAGTTTTT 1951 AGGTGCCATT ATTTTTAAAA AATTCTATAT TTCCAATGAA CGATGTTAGA 2001 TTTTACACAG AACATATTCT CTGCATGATT TCAGAAAAGA AAATCTAAAA 2051 AGGTAATACG GGTATTTCAA ATAAAATCCT TTCTGGTATG AAAGGCTCCA 2101 TTGATTTTAT TAAGCCTTCC TTTACCTTGT AGTACAAGGT GCTTTAATGG 2151 GATAGAACTA AGCATATCAA TATCTATAAC TGCATTTTGT GCTAGACAAT 2201 TACTGTTCTT TTCTCTAAAA TGTATATGTC AATTTACAAG GCCAGGGATA 2251 GAAAACACTC CATAATTGCT TTCCTTGATT TTGCTGAGGA TTTGGTATGA 2301 TTTTAGTAAG CAAACTGTTT TTTGGTTTTT CCTTAATGTT TTTAATTTTT 2351 TTTCCTCTTG CAACAATGAC GGTGCATGTT CTTATAAATA TAGGAAGGTC 2401 CAGATATAAA TAGTAACCTA AAGTTCTTGC TGTGCTTAAA AAAAAAAATC 2451 ATGTGGCTCT TTCAATATTT GAACTGCTAA GCAATGACAT CTGTAGTTTT 2501 ATCTCCTTTT TTATGTCATA GAAATTAATA TGATACTTTA AATATGTAAA 2551 TATAATACAT TGGTAATGCT ATTATTTATA TCTGTCTTAA CATAATTTAA 2601 GTTGTAGCTG TGTCTTGGAA ATATTTTTAA GGTAATCTAT ATTCACATTG 2651 CCTGTGTTAA TGCTTTTTAA GGTTTGTATA CATCAGATGT ATATTTTTGG 2701 TTTGGCATAA GCTACGATTG TAATTTTTCT TGGCTTTTTG TTCATAAAGA 2751 ATTTTTTGAA GGAATGGTAA CAAATGGTAA TTTACAAATG GTTGTGAATA 2801 AACACATTTT TACACTTAAA AAAAAAAAAA AAAAA BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 2 ORF from 272 bp to 1177 bp; peptide length: 302 Category: similarity to known protein 1 MGNCCWTQCF GLLRKEAGRL QRVGGGGGSK YFRTCSRGEH LTIEFENLVE 51 SDEGESPGSS HRPLTEEEIV DLRERHYDSI AEKQKDLDEK IQKELALQEE 101 KLRLEEEALY AAQREAARAA KQRKLLEQER QRIVQQYHPS NNGEYQSSGP 151 EDDFESCLRN MKSQYEVFRS SRLSSDATVL TPNTESSCDL MTKTKSTSGN 201 DDSTSLDLEW EDEEGMNRML PMRERSKTEE DILRAALKYS NKKTGSNPTS 251 ASDDΞNGLEW ENDFVSAEMD DNGNSEYSGF VNPVLELSDS GIRHSDTDQQ 301 TR BLASTP hits Entry A55817 from database PIR: cyclin-dependent kinase pl30-PITSLRE - mouse Length = 783 Score = 123 (43.3 bits). Expect = 0.00013, P = 0.00013 Identities = 53/197 (26%), Positives = 96/197 (48%) Alert BLASTP hits for DKFZphfbr2_2cl8, frame 2 No Alert BLASTP hits found Pedant information for DKFZphfbr2_2cl8, frame 2 Report for DKFZphfbr2_2cl8.2 [LENGTH] 302 [MW] 34281.39 [pi] 4.73 [PROSITE] MYRISTYL 5 [PROSITE] CK2 PHOSPHO SITE 12 [PROSITE] TYR PHOSPHO SITE 2 [PROSITE] PKC PHOSPHO SITE 3 [KW] All Alpha [KW] LOW COMPLEXITY 13.58 % [KW] COILED COIL 13.58 % SEQ MGNCCWTQCFGLLRKEAGRLQRVGGGGGSKYFRTCSRGEHLTIEFENLVESDEGESPGSS SEG xxxxx PRD ccccccccchhhhhhhhhheeecccccccceeeeccccccchhhhhhhhccccccccccc COILS SEQ HRPLTEEEIVDLRERHYDSIAEKQKDLDEKIQKELALQEEKLRLEEEALYAAQREAARAA SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD ccchhhhhhhhhhhccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC SEQ KQRKLLEQERQRIVQQYHPSNNGEYQSSGPEDDFESCLRNMKSQYEVFRSSRLSSDATVL SEG xxxxxxx PRD hhhhhhhhhhhhhhhhccccccccccccccccchhhhhhhhhhhheeeeecccccceeee COILS CCCCCCCCC SEQ TPNTESSCDLMTKTKSTSGNDDSTSLDLEWEDEEGMNRMLPMRERSKTEEDILRAALKYS SEG PRD ccccccccccccccccccccccccchhhhhhhccccccchhhhhhhcchhhhhhhhhhhc COILS SEQ NKKTGSNPTSASDDSNGLEWENDFVSAEMDDNGNSEYSGFVNPVLELSDSGIRHSDTDQQ SEG PRD cccccccccccccccccccccccceeeecccccccccccccceeeecccccccccccccc COILS SEQ TR SEG PRD CC COILS Prosite for DKFZphfbr2_2cl8.2 PS00005 60->63 PKC PHOSPHO SITE PDOC00005 PS00005 170->173 PKC PHOSPHO " SITE PDOC00005 PS00005 240->243 PKC PHOSPHO " " SITE PDOC00005 PS00006 36->40 CK2 PHOSPHO " SITE PDOC00006 PS00006 65->69 CK2 PHOSPHO SITE PDOC00006 PS00006 79->83 CK2 PHOSPHO SITE PDOC00006 PS00006 148->152 CK2 PHOSPHO " " SITE PDOC00006 PS00006 163->167 CK2 PHOSPHO- " SITE PDOC00006 PS00006 186->190 CK2 PHOSPHO " " SITE PDOC00006 PS00006 198->202 CK2 PHOSPHO " " SITE PDOC00006 PS00006 204->208 CK2 PHOSPHO- " SITE PDOC00006 PS00006 226->230 CK2 PHOSPHO " " SITE PDOC00006 PS00006 228->232 CK2 PHOSPHO " SITE PDOC00006 PS00006 250->254 CK2 PHOSPHO ' SITE PDOC00006 PS00006 295->299 CK2 PHOSPHO " " SITE PDOC00006 PS00007 103->111 TYR PHOSPHO " SITE PDOC00007 PS00007 103->111 TYR PHOSPHO " SITE PDOC00007 PS00008 24->30 MYRISTYL PDOC00008 PS00008 25->31 MYRISTYL PDOC00008 PS00008 199->205 MYRISTYL PDOC00008 PS00008 245->251 MYRISTYL PDOC00008 PS00008 291->297 MYRISTYL PDOC00008 (No Pfam data available for DKFZphfbr2_2cl8.2) DKFZphfbr2_2dl5 group: di erentiation/development DKFZphfbr2_2dl5 encodes a novel 438 amino acid protein similarity to Mus musculus testis- specific Y-encoded-like protein (Tspyll) . The TSPY genes are arranged in clusters on the Y chromosome of many mammalian species. TSPY is believed to function in early spermatogenesis and is a candidate for GBY, the putative gonadoblastoma-inducing gene on the Y. The novel protein is a new member of the TSPY-SET- NAP1L1 family, which represents proteins closely related to TSPY. Therefore, the new protein seems to be involved in early spermatogenesis. The new protein can find application in modulating early spermatogenesis. strong similarity to testis-specific Y-encoded-like protein complete cDNA, complete eds, EST hits localisation: primer B does not match perfect Sequenced by Qiagen Locus: /map="729.2 cR from top of Chr6 linkage group" Insert length: 3229 bp Poly A stretch at pos. 3206, polyadenylation signal at pos. 3184 1 GGAGACTGTA GGGTGGGCGG TGCGAGCGGC GGTTAGCTCC CAGTTCGGCC 51 TCTGAGGAAA ACGGGCGTTC GCCTGCGGTT GGTCCGACTG TTAGCAACAT 101 GAGCGGCCTG GATGGGGTCA AGAGGACCAC TCCCCTCCAA ACCCACAGCA 151 TCATTATTTC TGACCAAGTC CCGAGCGACC AGGACGCACA CCAGTACCTG 201 AGGCTCCGCG ACCAAAGCGA GGCGACACAG GTGATGGCGG AGCCGGGTGA 251 GGGAGGCTCG GAGACCGTCG CGCTCCCGCC TTCACCGCCT TCAGAGGAGG 301 GGGGCGTACC CCAGGATCCC GCGGGCCGTG GCGGTACTCC CCAGATCCGA 351 GTTGTTGGGG GTCGCGGTCA TGTGGCGATC AAAGCCGGGC AGGAAGAGGG 401 CCAGCCTCCC GCCGAAGGCC TGGCAGCCGC TTCTGTGGTG ATGGCAGCCG 451 ACCGCAGCCT GAAAAAGGGC GTTCAGGGTG GAGAGAAGGC CCTAGAAATC 501 TGTGGCGCCC AGAGATCCGC GTCTGAGCTG ACGGCGGGGG CGGAGGCTGA 551 GGCGGAGGAG GTGAAGACAG GAAAGTGCGC CACCGTCTCA GCAGCCGTGG 601 CTGAGAGGGA GAGCGCTGAG GTGGTGGTGA AGGAAGGCCT GGCGGAGAAG 651 GAGGTAATGG AGGAGCAGAT GGAGGTAGAG GAGCAGCCGC CAGAAGGTGA 701 AGAAATAGAA GTGGCGGAGG AGGATAGATT GGAGGAGGAG GCGAGGGAGG 751 AAGAAGGGCC CTGGCCTTTG CATGAGGCTC TCCGCATGGA CCCTCTGGAG 801 GCCATCCAGC TGGAACTGGA CACTGTGAAT GCTCAGGCCG ACAGGGCCTT 851 CCAACAGCTG GAGCACAAGT TTGGGCGGAT GCGTCGACAC TACCTGGAGC 901 GGAGGAACTA CATCATTCAG AATATCCCGG GCTTCTGGAT GACTGCTTTT 951 CGAAACCACC CCCAGTTGTC CGCCATGATT AGGGGCCAAG ATGCAGAGAT 1001 GTTAAGGTAC ATAACCAATT TAGAGGTGAA GGAACTCAGA CACCCTAGAA 1051 CCGGTTGCAA GTTCAAGTTC TTCTTTAGAA GAAACCCCTA CTTCAGAAAC 1101 AAGCTGATTG TCAAGGAATA TGAGGTAAGA TCCTCCGGCC GAGTGGTGTC 1151 TCTTTCTACT CCAATTATAT GGCGCAGGGG GCATGAACCC CAGTCCTTCA 1201 TTCGCAGAAA CCAAGACCTC ATCTGCAGCT TCTTCACTTG GTTTTCAGAC 1251 CACAGCCTTC CAGAGTCCGA CAAAATTGCT GAGATTATTA AAGAGGATCT 1301 GTGGCCAAAT CCACTGCAAT ACTACCTGTT GCGTGAAGGA GTCCGTAGAG 1351 CCCGACGTCG CCCGCTAAGG GAGCCTGTAG AGATCCCCAG GCCCTTTGGG 1401 TTCCAGTCTG GTTAACATTT GCCCTTGGGA ATACTCCTGC ACAAGGTCTC 1451 CTACCACCTT CTGCTGGACC TGTGCTTGGG CATCAGCAAT GAGTATGCCT 1501 TCTATTGTGC TTTGTTTTTG CTGACTTTTC TGCACCCTGT TTCCTTTGGA 1551 TATTCAGTTC TCTCAACCTC AAGATTGAGA CGGTGGTGGG TATGCTTCTC 1601 CACTTCCATA TGACCTTCAT GCTGTTCTGG AATATCACAT GCTACGAGGT 1651 CATCCTTCAC ACTACTTGTA AGCCAAGCAA ATGATACTGT AGATTGTACT 1701 GCCTTTATCT GCACTGCTTG GACCCTGTTT ATTCCCAGGG CCTCTGAACT 1751 GGTTGCTGTC ACTTGGATTT CTAGCTTTGG GAGCCTGTTC CACCTACTCA 1801 GCTCTGCATT GAGCAGTATG GGCACATGCC CTGTGGACAG TTACTGGACG 1851 TTAATGAACT CAGAGGAGAA AAGCAGTGAG CCACTTGTTC TGTGTGATTT 1901 ATGGTACTTC ATTGCTCTTC CTTCACCTCT AGTCACTTTC TATTGCTACC 1951 TGCCCTACAT TGGCTCCTGC CAAGGTCCCT CTCTCTCCCT GTTTTCCTTT 2001 TTTTGAGACG GAGGACGGAG TCTTGCTCTG 2051 TCGCCCAGGT TGGAGTGCAG TGGCGCGATC TCGGCTCACT GCAACCTCCA 2101 CCTCCCGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTAGCTGGGA 2151 CTACAGGCGC GCGCCGCCAC GCCCGGCTAA TTTTTATATT TTTAGTAGAG 2201 ACGGGGTTTC ACCATGCTGG CCAGGCTGGT CTCGAACCCC GACCTCGTGA 2251 TCCGCCCTCC TTAGCCTCCC AATCCTCTCT TAAAAAAGTG ATAGCTCAGA 2301 AATATTTGTA AAAGCAAGGT TTTTATTTCA TTTTGGCTCT GTCATTTTCA 2351 GAGGCAAAGA AGTTGGCCTG TAAAATAGAG TGCTAGAGCT CTTACGCCCC 2401 TCCCCTTCTT CCCAACTTCC TACTTCCTAG CCCTTTTATC AACTCCTAGA 2451 ATAGTTAAAG AGAGACACAT CTAGATGGGA TGAAAGGTGC CCTAAGCAGG 2501 AGAAACTGAA CAAAAGGCTA GAGGCATGGG CCAGGTAAAA ATTGGGCCTA 2551 GAGTGAAGAC TGTGCTGCCG TTAAGAGCTT TCGAGGAAGG AGTACTTACT 2601 CCCCAATGAT GATGAATGGA GAAATACTTT TCAGGGAGAA TTGAAGGGGT 2651 TAAAGTGTTA AATATGTTGC CTAGACAAGG GTTCTTTAAA GAAAGACAGC 2701 GCAACTTTGA ATGCTTTCTT ACTTGTTTTG TGACCTAATT TATGTGGAAG 2751 ATTGTTATTT CATTAGGATT TAGTAAAATT TTTTTTTCTG ATTCTAAACT 2801 TATTGTGAAA ATTGAGCTGT ACAGATATTC TTTTGATTTC AATTGGGAAC 2851 ATTTGGAAGA ACAACAGTCT TACTTGCCTG TACAATATAG AGACATATGA 2901 ATAGTCATAA CAGTTTTCAA CTTGTTCTTG TTTCTGTTAA ACTATATTCC 2951 TAGAAACATA GTTTGAACAA CTTGGTCTTT GTTAGGCTTG TCAAATTGCC 3001 TTCATGGAAA AATAATCTAC AAAAGTATGG TTTAATTGAT TGTCTTACAT 3051 GATAATTTTC CCTGGCAACA ACTTAGTAAG TGATATATCT TTTTTCCTAA 3101 ATTGCTTAAA TACTGTGAAA TTGCTCTGAC AAATTGGAAG TGTACCATTG 3151 GCATATTTGT CTTCCTTTTT ATGCATGATG GTAAAATAAA AGCATGTTGT 3201 TCTGCTAAGA AAAAAAAAAA AAAAAAAAA BLAST Results Entry AF042181 from database EMBLNEW: Homo sapiens testis-specific Y-encoded-like protein (TSPYL) mRNA, partial eds. Score = 3411, P = 6.9e-148, identities = 685/687 Entry HS938343 from database EMBL: human STS WI-11947. Score = 1195, P = 2.1e-46, identities = 273/299 Medline entries 98399864: Murine and human TSPYL genes: novel members of the TSPY-SET-NAPILI family Peptide information for frame 3 ORF from 99 bp to 1412 bp; peptide length: 438 Category: strong similarity to known protein Classification: Differentiation/Development 1 MSGLDGVKRT TPLQTHSIII SDQVPSDQDA HQYLRLRDQS EATQVMAEPG 51 EGGSETVALP PSPPSEEGGV PQDPAGRGGT PQIRVVGGRG HVAIKAGQEE 101 GQPPAEGLAA ASVVMAADRS LKKGVQGGEK ALEICGAQRS ASELTAGAEA 151 EAEEVKTGKC ATVSAAVAER ESAEVVVKEG LAEKEVMEEQ MEVEEQPPEG 201 EEIEVAEEDR LEEEAREEEG PWPLHEALRM DPLEAIQLEL DTVNAQADRA 251 FQQLEHKFGR MRRHYLERRN YIIQNIPGFW MTAFRNHPQL SAMIRGQDAE 301 MLRYITNLEV KELRHPRTGC KFKFFFRRNP YFRNKLIVKE YEVRSSGRVV 351 SLSTPIIWRR GHEPQSFIRR NQDLICSFFT WFSDHSLPES DKIAEIIKED 401 LWPNPLQYYL LREGVRRARR RPLREPVEIP RPFGFQSG BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_2dl5, frame 3 TREMBL:AF042180_1 gene: "Tspyll"; product: "testis-specific Y-encoded-like protein"; Mus musculus testis-specific Y-encoded-like protein (Tspyll) mRNA, complete eds., N = 1, Score = 1202, P = 3.1e-122 TREMBL:AB018264_1 gene: "KIAA0721"; product: "KIAA0721 protein"; Homo sapiens mRNA for KIAA0721 protein, partial eds., N = 1, Score = 798, P = 2e-79 TREMBL :AB015345_1 gene: "HRIHFB2216"; Homo sapiens HRIHFB2216 mRNA, partial eds., N = 1, Score = 570, P = 2.9e-55 >TREMBL:AF042180_1 gene: "Tspyll"; product: "testis-speci ic Y-encoded-like protein"; Mus musculus testis-specific Y-encoded-like protein (Tspyll) mRNA, complete eds . Length = 379 HSPs: Score = 1202 (180.3 bits), Expect = 3.1e-122, P = 3.1e-122 Identities = 258/377 (68%), Positives = 283/377 (75%) Query: 62 SPPSEEGGVPQDPAGR GGTPQIRVVGGRGHVAIKAGQEE—GQP-P—AEGLAA 110 SP +EG D G GTP R + G G+ G P P EGL Sbjct: 3 SPERDEGTPVPDSRGHCDADTVSGTPDRRPLLGEEKAVTGEGRAGIVGSPAPRDVEGLVP 62 Query: 111 ASVVMAADRSLKK-GVQGGEKALEICGAQRSASELTAGAEAEAEEVKTGKCATVΞAAVAE 169 V AA + V+G A+ + ++ T GAE++A +VKT + TV+AA Sbjct: 63 QIRVAAARQGESPPSVRGPAAAVFVTPKYVEKAQETRGAESQARDVKT-EPGTVAAAA— 119 Query: 170 RESAEVVVKEGLAEKEVMEEQMEVEEQPPEGEEIEVAEEDRLEEEAREEEGPWPLHEALR 229 E +EV EE MEVE Q P GEE+E+ E EA EE GPW L LR Sbjct: 120 -EKSEVATPGS EEVMEVE-QKPAGEEMEMLEAΞGGVREAPEEAGPWHLGIDLR 170 Query: 230 MDPLEAIQLELDTVNAQADRAFQQLEHKFGRMRRHYLERRNYIIQNIPGFWMTAFRNHPQ 289 +PLEAIQLELDTVNAQADRAFQ LE KFGRMRRHYLERRNYIIQNIPGFWMTAFRNHPQ Sbjct: 171 RNPLEAIQLELDTVNAQADRAFQHLEQKFGRMRRHYLERRNYIIQNIPGFWMTAFRNHPQ 230 Query: 290 LSAMIRGQDAEMLRYITNLEVKELRHPRTGCKFKFFFRRNPYFRNKLIVKEYEVRSSGRV 349 LSAMIRG+DAEMLRY+T+LEVKELRHP+TGCKFKFFFRRNPYFRNKLIVKEYEVRSSGRV Sbjct: 231 LSAMIRGRDAEMLRYVTSLEVKELRHPKTGCKFKFFFRRNPYFRNKLIVKEYEVRSSGRV 290 Query: 350 VSLSTPIIWRRGHEPQSFIRRNQDLICSFFTWFSDHSLPESDKIAEIIKEDLWPNPLQYY 409 VSLSTPIIWRRGHEPQSFIRRNQDLICSFFTWFΞDHSLPESD+IAEIIKEDLWPNPLQYY Sbjct: 291 VSLSTPIIWRRGHEPQSFIRRNQDLICSFFTWFSDHSLPESDRIAEIIKEDLWPNPLQYY 350 Query: 410 LLREGVRRARRRPLREPVEIPRPFGFQSG 438 L REG+RR RRRP+REPVEIPRPFGFQSG Sbjct: 351 LCREGIRRPRRRPIREPVEIPRPFGFQSG 379 Pedant information for DKFZphfbr2_2dl5, frame 3 Report for DKFZphfbr2_2dl5.3 [LENGTH] 438 [MW] 49307.65 [pi] 5.36 [HOMOL] TREMBL:AF042180_1 gene: "Tspyll"; product: "testis-specific Y-encoded-like protein"; Mus musculus testis-specific Y-encoded-like protein (Tspyll) mRNA, complete eds. le- 107 [FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YKR048c] le-07 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YKR048c] le-07 [FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YKR048c] le-07 [FUNCAT] 09.13 biogenesis of chromosome structure [S. cerevisiae, YKR048c] le-07 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YKR048c] le-07 [BLOCKS] BL00376F [PIRKW] nucleus 6e-39 [PIRKW] DNA binding 3e-06 [PIRKW] phosphoprotein 6e-39 [PIRKW] alternative splicing 6e-39 [KW] Alpha_Beta [KW] LOW COMPLEXITY 22.83 % SEQ MSGLDGVKRTTPLQTHSIIIΞDQVPSDQDAHQYLRLRDQSEATQVMAEPGEGGSETVALP SEG x PRD ccccccccccccccceeeeecccccccccchhhhhhhhchhhhhcccccccccceeeecc SEQ PSPPSEEGGVPQDPAGRGGTPQIRVVGGRGHVAIKAGQEEGQPPAEGLAAASVVMAADRS SEG xxxxxxxxx PRD ccccccccccccccccccccceeeeecccceeeeecccccccccchhhhhhhhhhhhhcc SEQ LKKGVQGGEKALEICGAQRSASELTAGAEAEAEEVKTGKCATVSAAVAERESAEVVVKEG SEG xxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxx . PRD ccccccccccceeeccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh SEQ LAEKEVMEEQMEVEEQPPEGEEIEVAEEDRLEEEAREEEGPWPLHEALRMDPLEAIQLEL SEG .xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhccccchhhhhhhhhhhhhhhcccccccchhhhhhhhhhhhhhhh SEQ DTVNAQADRAFQQLEHKFGRMRRHYLERRNYIIQNIPGFWMTAFRNHPQLSAMIRGQDAE SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccceeeeecccccccccccccchhh SEQ MLRYITNLEVKELRHPRTGCKFKFFFRRNPYFRNKLIVKEYEVRSSGRVVSLSTPIIWRR SEG PRD hhhhhhhhhhhhhcccccceeeeeeeccccccchhhhhhccccccccccccccceeeecc SEQ GHEPQSFIRRNQDLICSFFTWFSDHSLPESDKIAEIIKEDLWPNPLQYYLLREGVRRARR SEG xxxxxxxxxxx PRD ccccchhhhhhcccccceeeeeccccccccchhhhhhhhhcccccceeeeccccchhhhh SEQ RPLREPVEIPRPFGFQSG SEG xxxxxxxx PRD hccccccccccccccccc (No Prosite data available for DKFZphfbr2_2dl5.3) (No Pfam data available for DKFZphfbr2_2dl5.3) DKFZphfbr2_2dl7 group: transmembrane proteins DKFZphfbr2_2dl7 encodes a novel 292 ammo acid protein with similarity to a C. elegans hypothetical protein. One transmembrane region is predicted for the protein. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of brain-specific genes and as a new marker for neuronal cells. similarity to C. elegans hypothetical protein TRANSMEMBRANE 1 Sequenced by Qiagen Locus : unknown Insert length: 1009 bp Poly A stretch at pos. 990, polyadenylation signal at pos. 969 1 TGGGCCTGTG GCTGGGGGCA GAGCTCAGAC TGTCTTCTGA AGATTGATGT 51 CTATTTCCTT GAGCTCTTTA ATTTTGTTGC CAATTTGGAT AAACATGGCA 101 CAAATCCAGC AGGGAGGTCC AGATGAAAAA GAAAAGACTA CCGCACTGAA 151 AGATTTATTA TCTAGGATAG ATTTGGATGA ACTAATGAAA AAAGATGAAC 201 CGCCTCTTGA TTTTCCTGAT ACCCTGGAAG GATTTGAATA TGCTTTTAAT 251 GAAAAGGGAC AGTTAAGACA CATAAAAACT GGGGAACCAT TTGTTTTTAA 301 CTACCGGGAA GATTTACACA GATGGAACCA GAAAAGATAC GAGGCTCTAG 351 GAGAGATCAT CACGAAGTAT GTATATGAGC TCCTGGAAAA GGATTGTAAT 401 TTGAAAAAAG TATCTATTCC AGTAGATGCC ACTGAGAGTG AACCAAAGAG 451 TTTTATCTTT ATGAGTGAGG ATGCTTTGAC AAATCCACAG AAACTGATGG 501 TTTTAATTCA TGGTAGTGGT GTTGTCAGGG CAGGGCAGTG GGCTAGAAGA 551 CTTATTATAA ATGAAGATCT GGACAGTGGC ACACAGATAC CGTTTATTAA 601 AAGAGCTGTG GCTGAAGGAT ATGGAGTAAT AGTACTAAAT CCCAATGAAA 651 ACTATATTGA AGTAGAAAAG CCGAAGATAC ACGTACAGTC ATCATCTGAT 701 AGTTCAGATG AACCAGCAGA AAAACGGGAA AGAAAAGATA AAGTTTCTAA 751 AGTAACAAAG AAGCGACGTG ATTTCTATGA GAAGTATCGT AACCCCCAAA 801 GAGAAAAAGA AATGATGCAA TTGTATATCA GAGTGAGTGA GATCACTACT 851 TTCCTTTACT ATTTTCTTTA CCTTGTATAT ATTTTATTAT ATGTAGATTG 901 TTTTGTTTTT CTTCAAGAAT ATTAATTTCT TTATTTGTCA TCATTTATTT 951 CCCATGGTCG TCTACTTGGA TTAAATGGGT TTTTAAATTC AAAAAAAAAA 1001 AAAAAAAAA BLAST Results Entry 189937 from database EMBL: Sequence 11 from patent US 5723315. Score = 1083, P = 2.2e-42, identities = 223/231 Entry 189938 from database EMBL: Sequence 12 from patent US 5723315. Score = 875, P = 7.4e-33, identities = 175/175 \ Medline entries No Medline entry Peptide information for frame 2 ORF from 47 bp to 922 bp; peptide length: 292 Category: similarity to unknown protein Classification: unset 1 MSISLSSLIL LPIWINMAQI QQGGPDEKEK TTALKDLLΞR IDLDELMKKD 51 EPPLDFPDTL EGFEYAFNEK GQLRHIKTGE PFVFNYREDL HRWNQKRYEA 101 LGEIITKYVY ELLEKDCNLK KVSIPVDATE SEPKΞFIFMS EDALTNPQKL 151 MVLIHGSGVV RAGQWARRLI INEDLDSGTQ IPFIKRAVAE GYGVIVLNPN 201 ENYIEVEKPK IHVQSSSDSS DEPAEKRERK DKVSKVTKKR RDFYEKYRNP 251 QREKEMMQLY IRVSEITTFL YYFLYLVYIL LYVDCFVFLQ EY BLASTP hits Entry S67436 from database PIR: hypothetical protein - fission yeast (Schizosaccharomyces pombe) Length = 266 Score = 112 (39.4 bits), Expect = 0.00037, P = 0.00037 Identities = 33/147 (22%), Positives = 69/147 (46%) Entry CEY75B8A_12 from database TREMBLNEW: gene: "Y75B8A.31"; Caenorhabditis elegans cosmid Y75B8A Score = 327, P = 1.5e-29, identities = 72/140, positives = 93/140 Alert BLASTP hits for DKFZphfbr2_2dl7, frame 2 No Alert BLASTP hits found Pedant information for DKFZphfbr2_2dl7, frame 2 Report for DKFZphfbr2_2dl7.2 [LENGTH] 292 [MW] 34260.50 [pi] 5.50 [HOMOL] TREMBLNEW:AF064782_1 product: "unknown"; Mus musculus clone pEN87 unknown mRNA, partial eds. le-119 [KW] SIGNAL_PEPTIDE 19 [KW] TRANSMEMBRANE 1 [KW] LOW_COMPLEXITY 10.96 % SEQ MSISLSSLILLPIWINMAQIQQGGPDEKEKTTALKDLLSRIDLDELMKKDEPPLDFPDTL SEG . xxxxxxxxxxxxxx PRD ccchhhhhhchhhhhhhccccccccccchhhhhhhhhhhhhcchhhhhhccccccccccc MEM SEQ EGFEYAFNEKGQLRHIKTGEPFVFNYREDLHRWNQKRYEALGEIITKYVYELLEKDCNLK SEG PRD hhhhhhcccccceeeecccccceeecccccccchhhhhhhhhhhhhhhhhhhhhhhhhhe MEM SEQ KVSIPVDATESEPKSFIFMSEDALTNPQKLMVLIHGSGVVRAGQWARRLIINEDLDSGTQ SEG PRD eeeccccccccccceeeeeeccccccccceeeeeecccccchhhhhcccccccccccccc MEM SEQ IPFIKRAVAEGYGVIVLNPNENYIEVEKPKIHVQSSSDSSDEPAEKRERKDKVSKVTKKR SEG PRD chhhhhhhhccceeeeeccccceeeeeccceeeeccccccccchhhhhhhhhhhhhhhhh MEM SEQ RDFYEKYRNPQREKEMMQLYIRVSEITTFLYYFLYLVYILLYVDCFVFLQEY SEG xxxxxxxxxxxxxxxxxx PRD hhhhhhhcccchhhhhhhhhhhhheeeeehhhhhhhhhhhhheeeeeeeccc MEM MMMMMMMMMMMMMMMMMMMMM (No Prosite data available for DKFZphfbr2_2dl7.2) (No Pfam data available for DKFZphfbr2_2dl7.2) DKFZphfbr2_2d20 group: brain derived DKFZphfbr2_2d20 encodes a novel 197 amino acid protein with similarity to Synechocystis sp. P74594 hypothetιcal32.8 kD protein. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of brain-specific genes . similarity to Synechocystis sp. (PCC 6803) complete cDNA, complete eds, EST hits potential start at bp 67 matches kozak consensus ANCatgG Sequenced by Qiagen Locus: unknown Insert length: 1787 bp Poly A stretch at pos. 1768, polyadenylation signal at pos. 1743 1 TGGGGCGGCC GCGGCGGGAA CATGGAGGAG CTGCTGAGGC GCGAGCTGGG 51 CTGCAGCTCT GTCAGGGCCA CGGGCCACTC GGGGGGCGGG TGCATCAGCC 101 AGGGCCGGAG CTACGACACG GATCAAGGAC GAGTGTTCGT GAAAGTGAAC 151 CCCAAGGCGG AGGCCAGAAG AATGTTTGAA GGTGAGATGG CAAGTTTAAC 201 TGCCATCCTG AAAACAAACA CGGTGAAAGT GCCCAAGCCC ATCAAGGTTC 251 TGGATGCCCC AGGCGGCGGG AGCGTGCTGG TGATGGAGCA CATGGACATG 301 AGGCATCTGA GCAGTCATGC TGCAAAGCTT GGAGCCCAGC TGGCCGATTT 351 ACACCTTGAT AACAAGAAGC TTGGAGAGAT GCGCCTGAAG GAGGCGGGCA 401 CAGTGTGGAG AGGAGGTGGG CAGGAGGAAC GGCCCTTTGT GGCCCGGTTT 451 GGATTTGACG TGGTGACGTG CTGTGGATAC CTCCCCCAGG TGAATGACTG 501 GCAGGAGGAC TGGGTCGTGT TCTATGCCCG GCAGCGCATT CAGCCCCAGA 551 TGGACATGGT GGAGAAGGAG TCTGGGGACA GGGAGGCCCT CCAGCTTTGG 601 TCTGCTCTGC AGTAAAAGAT CCCTGACCTG TTCCGTGACC TGGAGATCAT 651 CCCAGCCTTA CTCCACGGGG ACCTCTGGGG TGGAAACGTA GCAGAGGATT 701 CCTCTGGGCC GGTGATTTTT GACCCAGCTT CTTTCTACGG CCACTCGGAA 751 TATGAGCTGG CAATAGCTGG CATGTTTGGG GGCTTTAGCA GCTCCTTTTA 801 CTCCGCCTAC CACGGCAAAA TCCCCAAGGC CCCAGGATTC GAGAAGCGCC 851 TTCAGTTGTA TCAGCTCTTT CACTACTTGA ACCACTGGAA TCATTTTGGA 901 TCGGGGTACA GAGGATCCTC CCTGAACATC ATGAGGAATC TGGTCAAGTG 951 AGCGGGCCTT ACTCTGGAAG GAGGTCTCAG AGGTTTCTCC ACAGTCCTCT 1001 TCTGGGCAAA TTCTTGTTTC TTCACATGCC GGACTAGCTT AAGACCAATG 1051 CAGTAGCTTA TTTCCAAGCC TTGCAAAGTA TATAATATCT AAGAGGAAAG 1101 GTTTTGTCAT CCCAGCGTTG TCCACTTTGT GGGGCTTTGT AGGTAGACGG 1151 AGCCACACTA CAGGCAGGGT ATGAGCAGAG GGATGTATGG AGTGTGGGCG 1201 ACTCTGAGCC TCACTGCTGC TGCAAGGTGG GGAAACTGTA AGTGAACCCC 1251 TGTGGGTGCG GGGGAGGGTA TCCGGTGCGC AGGGAGGTGG CCAGCGCCCC 1301 CGGGCACTGC TGCTCATAGG TACCTTTCCG CTGCCTCCTC CCTGCTCTCC 1351 TGTGCAGGAA TGTCTCTGAG CTGTTCACGT TGATGCTTCT TGGTTGGCAA 1401 GACTTGGGTG TAGACATGAA ACCACCTTAC TAAAAGCGTC TTAAAATGAC 1451 CAATTCCAGA ATCAAGCGTA TTCCGTTTTC CTCCTGCATG ATCCCTGGGC 1501 CCTCCCGCAG GCTGAGCAAG TCTGTAAACT GATTCTGGGA GAAACCAAGC 1551 TGCTGGCCGT AGGATGTCCT TGGGTACATC CAGGAGTCTT CATTGCTTCT 1601 GTTATTACCC CGTCTCCTCT GCCATTTTCT ACAGCTTGCT GAGTTGTCAT 1651 TCCTTTGCAA CATTAAAATA CATGCTGAAC TCATATTTTT CCTTCCTTCA 1701 CTGTTGTAGT AAAGAGACAT ATTTCATGAA TGGCATTGAT GCTAATAAAC 1751 CCTTTGCCCA AAAATTTGAA AAAAAAAAAA AAAAAAA BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 1 ORF from 22 bp to 612 bp; peptide length: 197 Category: similarity to unknown protein Prosite motifs: LEUCINE ZIPPER (117-139) 1 MEELLRRELG CSSVRATGHS GGGCISQGRS YDTDQGRVFV KVNPKAEARR 51 MFEGEMASLT AILKTNTVKV PKPIKVLDAP GGGSVLVMEH MDMRHLSSHA 101 AKLGAQLADL HLDNKKLGEM RLKEAGTVWR GGGQEERPFV ARFGFDVVTC 151 CGYLPQVNDW QEDWVVFYAR QRIQPQMDMV EKESGDREAL QLWSALQ BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_2d20, frame 1 No Alert BLASTP hits found Pedant information for DKFZphfbr2_2d20, frame 1 Report for DKFZphfbr2_2d20.1 [LENGTH] 197 [MW] 21963.25 [pi] 6.96 [HOMOL] PIR:S76790 hypothetical protein - Synechocystis sp. (strain PCC 6803) 9e-12 [SUPFAM] hypothetical protein bl725 le-06 [PROSITE] LEUCINE_ZIPPER 1 [PROSITE] MYRISTYL 2 [PROSITE] GLYCOSAMINOGLYCAN 1 [PROSITE] PKC_PHOSPHO_SITE 2 [KW] Alpha_Beta SEQ MEELLRRELGCSSVRATGHSGGGCISQGRSYDTDQGRVFVKVNPKAEARRMFEGEMASLT PRD ccchhhhhccccceeeeccccccceeeccccccccceeeeeeccchhhhhhhhhhhhhhh SEQ AILKTNTVKVPKPIKVLDAPGGGSVLVMEHMDMRHLΞSHAAKLGAQLADLHLDNKKLGEM PRD hhhhhheeeeccceeeecccccceeeeecccccccchhhhhhhhhhhhhhhcccccchhh SEQ RLKEAGTVWRGGGQEERPFVARFGFDVVTCCGYLPQVNDWQEDWVVFYARQRIQPQMDMV PRD hhhhhccccccccccccceeeccccceeeccccccccccccchhhhhhhhhhhhhhhhhh SEQ EKEΞGDREALQLWSALQ PRD hhhccchhhhhhhhccc Prosite for DKFZphfbr2_2d20.1 PS00002 20->24 GLYCOSAMINOGLYCAN PDOC00002 PS00005 13->16 PKC_PHOSPHO_ΞITE PDOC00005 PS00005 67->70 PKC_PHOSPHO_SITE PDOC00005 PS00008 22->28 MYRISTYL PDOC00008 PS00008 104->110 MYRISTYL PDOC00008 PS00029 96->118 LEUCINE ZIPPER PDOC00029 (No Pfam data available for DKFZphfbr2_2d20.1) DKFZphfbr2_2gl8 group: brain derived DKFZphfbr2_2gl8 encodes a novel 229 amino acid protein with partial similarity to the humane dJ30M3.2 gene product. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of bram-specific genes . J30M3.2 extension of genmodel complete cDNA, complete eds, EST hits (mouse ESTs with >90% Identities) Sequenced by Qiagen Locus: /map="6p22.1-22" Insert length: 2444 bp Poly A stretch at pos. 24 5, no polyadenylation signal found 1 TGGTCGAGGG TCGACGGTAT CGATAAGTTT TTTTTTTTTT TTTTTTTTTT 51 TGGAAAGCAA GGATCACACT TCCCCCTCCC TGTTCCTTAA TCCCTTTTCT 101 AAAAAGGGGG GAAAATCCGG ATGGATTTTA GGGATTGGTC TGGTGTCAGC 151 TGTGTCTTAT TGCACACCTA AATCCTGATT ATAGGCTTTT CATTTCTCCG 201 CAAAGCCTTT ATTTTGGCAG TTAAGCCAAA TGTGTTTTCC AGAAAGTTAG 251 TTATTTTCTC CTCTTTCTTT CCTTTCTTTC CTCCCTTTTT CCCGTCTGAC 301 CCCAAACGTT ATTGTCCAAA CATGACTGGA CAGCAGCTTT TGTTTCTTGA 351 CCCTGTAATA TGACAGTCTG CTAATATTGA CAGAAGGTGC AGTTTTTGGG 401 TTATAGTCGT GATTTTCGCT AATCAATCAT ATTAGCAGGA AAAAAAATGA 451 CTTGTTTCTG TTGTACTTGA GTCTTAAGAA AAAGTGCCCA TAGTTTAGTG 501 ACAATTTCCA AAGGCTTTAG TACCACCTGT ATTTCAAAAT GGGGGACCCA 551 AACTCCCGGA AGAAACAAGC TCTGAACAGA CTACGTGCTC AGCTTAGAAA 601 GAAAAAAGAA TCTCTAGCTG ACCAGTTTGA CTTCAAGATG TATATTGCCT 651 TTGTATTCAA GGAGAAGAAG AAAAAGTCAG CACTTTTTGA AGTGTCTGAG 701 GTTATACCAG TCATGACAAA TAATTATGAA GAAAATATCC TGAAAGGTGT 751 GCGAGATTCC AGCTATTCCT TGGAAAGTTC CCTAGAGCTT TTACAGAAGG 801 ATGTGGTACA GCTCCATGCT CCTCGATATC AGTCTATGAG AAGGGATGTA 851 ATTGGCTGTA CTCAGGAGAT GGATTTCATT CTTTGGCCTC GGAATGATAT 901 TGAAAAAATC GTCTGTCTCC TGTTTTCTAG GTGGAAAGAA TCTGATGAGC 951 CTTTTAGGCC TGTTCAGGCC AAATTTGAGT TTCATCATGG TGACTATGAA 1001 AAACAGTTTC TGCATGTACT GAGCCGCAAG GACAAGACTG GAATCGTTGT 1051 CAACAATCCT AACCAGTCAG TGTTTCTCTT CATTGACAGA CAGCACTTGC 1101 AGACTCCAAA AAACAAAGCT ACAATCTTCA AGTTATGCAG CATCTGCCTC 1151 TACCTGCCAC AGGAACAGCT CACCCACTGG GCAGTTGGCA CCATAGAGGA 1201 TCACCTCCGT CCTTATATGC CAGAGTAGAG TACTGACCAG CAAAATGGAG 1251 AAGATCAGAG AATGCAGCAG CAGTTTTTTT TCTTGTTTTC TTACCACTTT 1301 ATTCTTTCAG AGTTTAAAGA AAATGGACTC ATGCACAGAA CACTATGCAT 1351 TTTGAAACTT GTTCATCCTG GATTTTTTTA AATCATTTTT ATCTCAGAAC 1401 TTAAACAAAA ATTAGATGTC GTGCACGGAC TGTGTGAAAG AAGATGCTTT 1451 GCATATTTGC TGCACTGCAT CAGTATCTTA CTAAAAATGT GAAATGAAAG 1501 GACTATTGTA CACTGAAATG CTTAAATGTA TCTGAAAGCA CAAGGTGATA 1551 CTCATTTTTA TGGTCTTCCC ATTTGTGCTG GTTTTTGCCT CTTTGACATC 1601 TGTCATCAGT ATTTAGAGGG TGAGAAGTGA ATGTAACAGG TATAAATAAC 1651 ATTTTTAAAA ACAATAACTT TGCTATAATC ACAGTTGTTC CAGAGCACTG 1701 TCAGATACAT TCTAATGACC AGAACTGGTT TAAAAAAAGA AAATACAACC 1751 ATGGGAAAGA AATCTTAAAT GAAAAACGCA TCTCATTGTA GGCATTTTTG 1801 CCTCATATTT TACTGGGCCA TGTTTGTTTC CTGGTACTCA TGTATTTTTT 1851 TTTTTTCCAG ATCTCTTTCC CCAAGTTGCT ATTGTAAGAG TATTCTGCTG 1901 CGTGTGGATG CAGTTATACA CATTAAAGCA GATCTGGAGT CTGAAGTAGC 1951 TATAAAGCAG CTATAAAACA GAAATACATG CATAGCTGCA GAAACCATGA 2001 TAGGTAGAGG ACTTTTCTTT TGGTTTTGTT TTGTTTTGTT TTGTTTTGTT 2051 TTTGGTTTTA CAGAGAAGAG ATTTTTATTA CAAAGAAAAA AATTCCAGTG 2101 AATTGTGCAG AAATGCTGGT TTTTACACCA TCCTAAAGAA AAACTTTACA 2151 AGGGTGTTTT GGAGTAGAAA AAAGGTTATA AAGTTGGAAT CTTAAATTGT 2201 AAAATTAACC ATTGAGTGTC AAAGTTCTAA AAGCAGAACT CATTTCGTGC 2251 AATGAACATA AGGAAAGACT ACTGTATAGG TTTTTTTTTT TCTCCTTTTA 2301 AATGAAGAAA AGCTTTGCTT AAGGGTTGCA TACTTTTATT GGAGTAAATC 2351 TGAATGATCC TACTCCTTTG GAGTAAGACT AGTGCTTACC AGTTTCCAAT 2401 TGTATTTAGC TTCTGTTGGA ATTTGAAAAA AAAAAAAAAA AAAA BLAST Results Entry HS338352 from database EMBL: human STS EST171398. Score = 1747, P = 3.0e-74, identities = 359/365 Entry HS447255 from database EMBL: human STS SHGC-10143. Score = 1717, P = 6.5e-73, identities = 365/383 Entry HS30M3 from database EMBLNEW: Human DNA sequence from clone 30M3 on chromosome 6p22.1-22.3. Contains three novel genes, one similar to C. elegans Y63D3A.4 and one similar to (predicted) plant, worm, yeast and archaea bacterial genes, and the first exon of the KIAA0319 gene. Contains ESTs, GSSs and putative CpG islands . Score = 6646, P = O.Oe+00, identities = 1344/1355 Medline entries No Medline entry Peptide information for frame 2 ORF from 539 bp to 1225 bp; peptide length: 229 Category: putative protein 1 MGDPNSRKKQ ALNRLRAQLR KKKESLADQF DFKMYIAFVF KEKKKKSALF 51 EVSEVIPVMT NNYEENILKG VRDSSYSLES SLELLQKDVV QLHAPRYQSM 101 RRDVIGCTQE MDFILWPRND IEKIVCLLFS RWKESDEPFR PVQAKFEFHH 151 GDYEKQFLHV LSRKDKTGIV VNNPNQSVFL FIDRQHLQTP KNKATIFKLC 201 SICLYLPQEQ LTHWAVGTIE DHLRPYMPE BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_2gl8, frame 2 TREMBLNEW:HS30M3_2 gene: "dJ30M3.2"; product: "dJ30M3.2 (novel protein)"; Human DNA sequence from clone 30M3 on chromosome 6p22.1-22.3. Contains three novel genes, one similar to C. elegans Y63D3A.4 and one similar to (predicted) plant, worm, yeast and archaea bacterial genes, and the first exon of the KIAA0319 gene. Contains ESTs, GSSs and putative CpG islands., N = 1, Score = 470, P = l.le-44 >TREMBLNEW:HS30M3_2 gene: "dJ30M3.2"; product: "dJ30M3.2 (novel protein)"; Human DNA sequence from clone 30M3 on chromosome 6p22.1-22.3. Contains three novel genes, one similar to C. elegans Y63D3A.4 and one similar to (predicted) plant, worm, yeast and archaea bacterial genes, and the first exon of the KIAA0319 gene. Contains ESTs, GSSs and putative CpG islands. Length = 86 HSPs: Score = 470 (70.5 bits), Expect = l.le-44, P = l.le-44 Identities = 86/86 (100%), Positives = 86/86 (100%) Query: 144 AKFEFHHGDYEKQFLHVLSRKDKTGIVVNNPNQSVFLFIDRQHLQTPKNKATIFKLCSIC 203 AKFEFHHGDYEKQFLHVLSRKDKTGIVVNNPNQSVFLFIDRQHLQTPKNKATIFKLCSIC Sbjct: 1 AKFEFHHGDYEKQFLHVLSRKDKTGIVVNNPNQSVFLFIDRQHLQTPKNKATIFKLCSIC 60 Query: 204 LYLPQEQLTHWAVGTIEDHLRPYMPE 229 LYLPQEQLTHWAVGTIEDHLRPYMPE Sbjct: 61 LYLPQEQLTHWAVGTIEDHLRPYMPE 86 Pedant information for DKFZphfbr2_2gl8, frame 2 Report for DKFZphfbr2_2gl8.2 [LENGTH] 229 [MW] 27083.42 [pi] 9.04 [HOMOL] TREMBL:HS30M3 2 gene "dJ30M3.2 " ; product : "dJ30M3 .2 (novel protein) ' Human DNA sequence from clone 30M3 on chromosome 6p22.1-22.3. Contains three novel genes, one similar to C. elegans Y63D3A.4 and one similar to (predicted) plant, worm, yeast and archaea bacterial genes, and the first exon of the KIAA0319 gene. Contains ESTs, GSSs and putative CpG islands. 6e-47 [PROSITE] MYRISTYL 2 [PROSITE] CAMP_PHOSPHO_SITE 2 [PROSITE] CK2_PHOSPHO_SITE 4 [PROSITE) TYR_PHOSPHO_SITE 1 [PROSITE] PKC_PHOSPHO_SITE 4 [PROSITE] ASN_GLYCOSYLATION 1 [KW] Alpha_Beta [KW] LOW COMPLEXITY 5.24 % SEQ MGDPNSRKKQALNRLRAQLRKKKESLADQFDFKMYIAFVFKEKKKKSALFEVSEVIPVMT SEG PRD cccccchhhhhhhhhhhhhhhhhhhhhhchhhhhhhhhhhhhhhhhhhhhhhhheeeeec SEQ NNYEENILKGVRDSSYSLESSLELLQKDVVQLHAPRYQΞMRRDVIGCTQEMDFILWPRND SEG xxxxxxxxxxxx PRD cchhhhhhhcccccccccchhhhhhhhhhhhhhccccccccceeecccccceeeecccch SEQ IEKIVCLLFSRWKESDEPFRPVQAKFEFHHGDYEKQFLHVLSRKDKTGIVVNNPNQSVFL SEG PRD hhhhhhhhhhhccccccccccccccccccccchhhhhhhhhhhcccceeeeccccceeee SEQ FIDRQHLQTPKNKATIFKLCSICLYLPQEQLTHWAVGTIEDHLRPYMPE SEG PRD eeecccccccccceeeeeeeeeeeeeccccccccceeeecccccccccc Prosite for DKFZphfbr2_2gl8.2 PS00001 175->179 ASN_GLYCOSYLATION PDOC00001 PS00004 22->26 CAMP_PHOΞPHO_SITE PDOC00004 PS00004 44->48 CAMP_PHOSPHO_SITE PDOC00004 PS00005 6->9 PKC_PHOSPHO_SITE PDOC00005 PS00005 99->102 PKC_PHOSPHO_SITE PDOC00005 PS00005 162->165 PKC_PHOSPHO_SITE PDOC00005 PS00005 189->192 PKC_PHOSPHO_SITE PDOC00005 PS00006 25->29 CK2_PHOSPHO_SITE PDOC00006 PS00006 80->84 CK2_PHOSPHO_SITE PDOC00006 PS00006 162->166 CK2_PHOSPHO_SITE PDOC00006 PS00006 218->222 CK2_PHOSPHO_SITE PDOC00006 PS00007 69->77 TYR_PHOSPHO_SITE PDOC00007 PS00008 70->76 MYRISTYL PDOC00008 PS00008 168->174 MYRISTYL PDOC00008 (No Pfam data available for DKFZphfbr2_2gl8.2) DKFZphfbr2_2hl group: brain derived DKFZphfbr2_2hl encodes a novel 180 amino acid protein with weak similarity to C. elegans D2007.4 protein No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of brain-specific genes . similarity to C. elegans D2007.4 protein CpG island m 5' region, complete cDNA Sequenced by Qiagen Locus : unknown Insert length: 957 bp Poly A stretch at pos. 939, polyadenylation signal at pos. 916 1 GGGGGTCCCT GACTTTATAT GGCTGCTCCT GGCGAGCGAC TGAGTCGTCC 51 GTGAGGAAAA AGAGGCGAGG CTTTTCCGAG ATCGTCTCAG CGATGGCGCT 101 TCGGTCGCGG TTTTGGGGGT TGTTCTCGGT TTGCAGGAAC CCTGGGTGCA 151 GGTTCGCAGC CCTGTCAACC AGCTCCGAGC CGGCAGCGAA ACCTGAAGTG 201 GACCCTGTGG AAAATGAAGC TGTCGCCCCA GAATTCACCA ACCGGAACCC 251 CCGGAACCTG GAGCTTTTGT CTGTAGCCAG GAAAGAGCGG GGCTGGCGGA 301 CGGTGTTTCC CTCCCGTGAG TTCTGGCACA GGTTGCGAGT TATAAGGACT 351 CAGCATCATG TAGAAGCACT TGTGGAGCAT CAGAATGGCA AGGTTGTGGT 401 TTCGGCCTCC ACTCGTGAGT GGGCTATTAA AAAGCACCTT TATAGTACCA 451 GAAATGTGGT GGCTTGTGAG AGTATAGGAC GAGTGCTGGC ACAGAGATGC 501 TTAGAGGCGG GAATCAACTT CATGGTCTAC CAACCAACCC CGTGGGAGGC 551 AGCCTCAGAC TCGATGAAAC GACTACAAAG TGCCATGACA GAAGGTGGTG 601 TGGTTCTACG GGAACCTCAG AGAATCTATG AATAAATGGA AGCATTAATT 651 GTTTTGAACA TGTAAATATA AATCTGTCAG CCACTACAGC CATCAAAAGA 701 GAGCATCTGG AAGAACAGCC AGCTTGGAAG TTTTACAGCA ATAATGTTGC 751 AGTGGAATAT TATTTGTAGT TAAGGTCATC CTCCTCCCCT TTCTGTTTTT 801 TTAAATCAAG AACTACGTTC TGCCCCTCTC TTGGGCTTCA GAAGCATCTA 851 AGAAAAGCAG TCATCAATTA TAATTAACTT TCAAAGGGCA AGTCAGAAGT 901 TGTTTATAAA TTACAAAATA AAGGCATATT ATGAACTCTA AAAAAAAAAA 951 AAAAAAA BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 3 ORF from 93 bp to 632 bp; peptide length: 16 Category: similarity to known protein Classif cation: unset 1 MALRSRFWGL FSVCRNPGCR FAALSTSSEP AAKPEVDPVE NEAVAPEFTN 51 RNPRNLELLS VARKERGWRT VFPΞREFWHR LRVIRTQHHV EALVEHQNGK 101 VVVSASTREW AIKKHLYSTR NVVACEΞIGR VLAQRCLEAG INFMVYQPTP 151 WEAASDSMKR LQSAMTEGGV VLREPQRIYE BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_2hl, frame 3 PIR:S44789 D2007.4 protein - Caenorhabditis elegans, N = 1, Score = 194, p = 2e-15 PIR:JC5753 ribosomal protein L18 - Vibrio proteolyticus, N = 1, Score = 121, P = 1. le-07 >PIR:S44789 D2007.4 protein - Caenorhabditis elegans Length = 170 HSPs: Score = 194 (29.1 bits), Expect = 2.0e-15, P = 2.0e-15 Identities = 51/134 (38%), Positives = 78/134 (58%) Query: 48 FTNRNPRNLELLSVARKERGWRTVFP—SREFWHRLRVIRTQHHVEA-LVEHQNGKVVVS 104 F NRNPRN EL+ G++ +R + +++ ++ + H E LV +Q+G VV+S Sbjct: 9 FVNRNPRNNELMGRQAPNTGYQFEKDRAARSYIYKVELVEGKSHREGRLVHYQDG-VVIS 67 Query: 105 ASTREWAIKKHLYSTRNVVACESIGRVLAQRCLEAGINFMVYQPTPWEAASDSMKRLQ— 162 AST+E +1 LYS + A +IGRVLA RCL++GI+F + T EA S + Sbjct: 68 ASTKEPSIASQLYSKTDTSAALNIGRVLALRCLQΞGIHFAMPGATK-EAIEKΞQHQTHFF 126 Query: 163 SAMTEGGVVLREPQRI 178 A+ E G+ L+EP + Sbjct: 127 KALEEEGLTLKEPAHV 142 Pedant information for DKFZphfbr2_2hl, frame 3 Report for DKFZphfbr2_2hl .3 [LENGTH] 180 [MW] 20576.57 [pi] 9.63 [HOMOL] PIR:S44789 D2007.4 protein - Caenorhabditis elegans 2e-13 [FUNCAT] j mrna translation and ribosome biogenesis [H. influenzae, HI0794] 2e-04 [SUPFAM] Escherichia col ribosomal protein L18 8e-06 [KW] Alpha_Beta SEQ MALRSRFWGLFSVCRNPGCRFAALSTSSEPAAKPEVDPVENEAVAPEFTNRNPRNLELLS PRD ccccccceeeeeeeecccccceeeecccccccccccccccceeeecccccccccchhhhh SEQ VARKERGWRTVFPSREFWHRLRVIRTQHHVEALVEHQNGKVVVSASTREWAIKKHLYSTR PRD hhhhcccccccchhhhhhhhhhccccchhhhhhhhhcccceeeeechhhhhhhhhhhhcc SEQ NVVACESIGRVLAQRCLEAGINFMVYQPTPWEAASDSMKRLQSAMTEGGVVLREPQRIYE PRD ccceeehhhhhhhhhhhhhcceeeeeccccchhhhhhhhhhhhhhhccceeecccccccc (No Prosite data available for DKFZphfbr2_2hl .3) (No Pfam data available for DKFZphfbr2_2hl .3) DKFZphfbr2_2hlO group: brain derived DKFZphfbr2_2hlO encodes a novel 220 amino acid protein without similarity to known proteins. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of bram-specific genes . unknown complete cDNA, complete eds, EST hits Sequenced by Qiagen Locus : unknown Insert length: 2176 bp Poly A stretch at pos. 2161, polyadenylation signal at pos. 2143 1 TGGGGAGTAT TCTAATTATA TTTTATATTT AATAAATTAT TTTTCTATTT 51 CTTTGTTATA TTAAGTTGCA CACTTGTTTC TTTTATCCAG AAAGTTTAGT 101 ATAATAAAAA TAGTTTTAAG ATTAACTGTG AATGTAAAGG AAAAGTATTA 151 TTAATTATTT CAGGAAATTG CAAGACCTAA CATGGCTGAA AGAGAAACAG 201 AAACATCAAA TTCTGAAAGT AAACAAGATA AAGCTGCTTC TTCAAAAGAA 251 AAAAATGGAT GTAATGCAAA TTCATTTGAA GGCTCATCAA CAACAAAAAG 301 TGAAGAAAGC ATAACAGTTT CAGATAAGGA AAATGAAACC TGTCTTGCAG 351 ACCAGGAAAC TGGCTCAAAA AACATCGTCA GTTGTGATTC AAATATTGGT 401 GCAGATAAAG TGGAAAAGAA AAAACAAATA CAACACGTTT GTCAGGAAAT 451 GGAGTTGAAG ATGTGCCAGA GTTCAGAAAA CATAATCTTA TCTGATCAGA 501 TTAAAGATCA CAACTCCAGT GAAGCCAGAT TTTCTTCAAA GAATATTAAG 551 GATTTGCGAT TAGCATCAGA TAATGTAAGC ATTGATCAGT TTTTGAGAAA 601 AAGACATGAA CCTGAATCTG TTAGTTCTGA TGTTAGCGAG CAAGGCAGTA 651 TTCATTTGGA ACCTCTGACT CCATCCGAGG TACTTGAGTA TGAAGCCACA 701 GAGATTCTTC AGAAAGGTAG TGGTGATCCT TCAGCCAAGA CTGATGAAGT 751 AGTGTCTGAT CAAACAGATG ACATTCCTGG AGGAAATAAC CCTAGCACAA 801 CAGAGGCAAC AGTAGACCTG GAAG TGAAA AAGAAAGAAG TTGAAATTAG 851 TCATTTTAAG TTTCAGTGTA CCAACGATAA GGGCATTTGG AACAGTGCTA 901 TCAGGTGAGC TCAGTGGTGC TGTTGTAGGT TCAGAAATGG AAATATGTAA 951 GGGAGGTCAC ACATACACTT TACCTGTATG TTCAACCTAT GTTATCAAAC 1001 AAACCAATTC ACCAATAATA GCATGATTAG TAGGGATTCC CAAAAAGTTT 1051 TTAAAAACAC GAACAGGATT TTAATGATAA TTAAATTTGC AGTGGAAAGG 1101 TCTCATTTAA TGGTTTTCAA GGAAATGGGA TTTGGTTGCT GACATGAATT 1151 GATGATATTA GTAATATTTA TAAAGCCTTT CAAACTTCCA TCAATCCTAA 1201 GCTAAAAATC TTTATTACCT GTATATCCTT TTCAGTTAAC TGAGAGGAAG 1251 GGATTTGGAA ACCATGTACT TTTGGGGAGT AATTGATTAA AAACAATGGC 1301 TGATTGGCAT TGTTAATGAA GGCTTTATTT GTGAGGATGA TGCTGGTAAA 1351 TGGAGCATGC TTAGAGTACT AAATTGATCT AATGAGAATT TGGATGAACA 1401 TAAACTTAAT TTTGGATTTA ATATAACATT CCAGTCAGAC GCATGTAAAC 1451 AGAATATTTG AATCTTTGTA CCTCCATACA AGTGTTAGCC TGCCAGGCTG 1501 TAAGCTTACC TTAATTAAAC TTTCAGTGAA AGTGGAATTA TTAAGATATA 1551 AATTTATATT TGTGCTTTTT GTCAGTGTGT AAGCTGTGTA GAAATTCTTT 1601 GATGTATTAG TTGTATTAAT GTAAAGTAGA AACCCATTGT TGAAACTCCT 1651 GTAGCTATTA TGCTTTTAAT ATTGTTTTAA TGTTCTTCCT TAGAAATAGG 1701 CCCATAAAAA TGGTCTGGAA GCCAAACCAA AGTATGGTAT AATGTAGATA 1751 TTGTAAAGCA GTAAACTGAA AACATGTCCT GGCATGTATT CAGCCATGTT 1801 TAAGTGACTT TTCTGTAATT GTAAAATAAA AACTTCAAAT GGGACCTAAA 1851 ACAGTGATGT AAAAGAACTG GTTTTGGAAA TTTAGCCTAA TTTATCTATA 1901 AGATGGCTGC TAAATTGATT TTTCAGTTCT TTTTATCATC TAAAATATAA 1951 TAGATATAGA AATGAATAAT ATGAAGAACA GTAGTTTGCT TTGAAATACT 2001 AATAAACTTT TATTTAAGAT GCTTCATTTT TACTTCTTAA AACGTGCTTT 2051 GGATTCTTAA ATTTTGTTTC ACTGAATGTT CAATGTTTTA AATGGCGATT 2101 AAAATACTCT GCTGTATATA GTAGTTTTTG AGTAAATATT TGCAATAAAA 2151 ATCTGCCCCC GAAAAAAAAA AAAAAA BLAST Results Entry G35287 from database EMBL: human STS SHGC-37375. Score = 2163, P = 2.8e-91, identities 437/441 Medlme entries No Medlme entry Peptide information for frame 2 ORF from 182 bp to 841 bp; peptide length: 220 Category: putative protein 1 MAERETETSN SESKQDKAAS SKEKNGCNAN SFEGSSTTKS EESITVSDKE 51 NETCLADQET GSKNIVSCDS NIGADKVEKK KQIQHVCQEM ELKMCQSSEN 101 IILSDQIKDH NSSEARFSSK NIKDLRLASD NVSIDQFLRK RHEPESVSSD 151 VSEQGSIHLE PLTPSEVLEY EATEILQKGS GDPSAKTDEV VSDQTDDIPG 201 GNNPSTTEAT VDLEDEKERS BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_2hlO, frame 2 No Alert BLASTP hits found Pedant information for DKFZphfbr2_2hlO, frame 2 Report for DKFZphfbr2_2hlO .2 [LENGTH] 220 [MW] 24109.02 [pi] 4.51 [FUNCAT] 04.99 other transcription activities [S. cerevisiae, YKR092c] 4e-05 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YKR092c] 4e-05 [PROSITE] MYRISTYL 3 [PROSITE] CK2_PHOSPHO_SITE 8 [PROSITE] PKC_PHOSPHO_SITE 5 [PROSITE] ASN_GLYCOSYLATION 3 [PFAM] TNFR/NGFR cysteine-rich region [KW] Alpha_Beta SEQ MAERETETSNSESKQDKAASSKEKNGCNANSFEGSSTTKSEESITVSDKENETCLADQET PRD cccccccccccccchhhhhhhhccccccccccccccccceeeeeeeeccccccccccccc SEQ GSKNIVSCDSNIGADKVEKKKQIQHVCQEMELKMCQSSENIILSDQIKDHNSSEARFSΞK PRD cccceeeecccccchhhhhhhhhhhhhhhhhhhhhhccceeeeccccccccccccccccc SEQ NIKDLRLASDNVSIDQFLRKRHEPESVΞSDVSEQGSIHLEPLTPSEVLEYEATEILQKGS PRD cchhhhhhcccchhhhhhhhcccccccccccccccceeecccccccchhhhhhhcccccc SEQ GDPΞAKTDEVVSDQTDDIPGGNNPSTTEATVDLEDEKERS PRD ccccccccccccccccccccccccccceeeehhhhhhccc Prosite for DKFZphfbr2_2hlO .2 PS00001 51->55 ASN_GLYCOSYLATION PDOC00001 PS00001 111->115 ASN_GLYCOSYLA ION PDOC00001 PS00001 131->135 ASN_GLYCOSYLATION PDOC00001 PS00005 20->23 PKC_PHOSPHO_SITE PDOC00005 PS00005 37->40 PKC_PHOSPHO_SITE PDOC00005 PS00005 47->50 PKC_PHOSPHO_SITE PDOC00005 PS00005 118->121 PKC_PHOSPHO_SITE PDOC00005 PS00005 184->187 PKC_PHOSPHO_SITE PDOC00005 PS00006 9->13 CK2_PHOSPHO_SITE PDOC00006 PS00006 13->17 CK2_PHOSPHO_SITE PDOC00006 PS00006 20->24 CK2_PHOSPHO_SITE PDOC00006 PS00006 38->42 CK2_PHOSPHO_SITE PDOC00006 PS00006 45->49 CK2_PHOSPHO_SITE PDOC00006 PS00006 47->51 CK2_PHOSPHO_SITE PDOC00006 PS00006 163->167 CK2_PHOSPHO_SITE PDOC00006 PS00006 205->209 CK2_PHOSPHO_SITE PDOC00006 PS00008 26->32 MYRISTYL PDOC00008 PS00008 34->40 MYRISTYL PDOC0000S PS00008 201->207 MYRISTYL PDOCOOOOE Pfam for DKFZphfbr2_2hlO .2 HMM_NAME TNFR/NGFR cysteine-rich region HMM *CpeG. tYtD.WNHvpqClpCtrCePEMGQYMvqPCTwTQNTVC* +E+ T +D +N ++C E G+ + +C+++ + Query 40 SEESITVSDKEN--ETC—LADQET—GSKNIVSCDSNIGADK 76 DKFZphfbr2 2ιl7 group: intracellular transport and trafficking DKFZphfbr2_2ιl7.3 encodes a novel 201 amino acid putative GTP-b ding protein related to RablB. Rab proteins are members of the Ras superfamily of GTPases. Rab proteins are localised to the cytoplasmic side of organelles and vesicles involved in the secretory (biosynthetic) and endocytotic pathways in eukaryotic cells. Rab proteins direct the targeting and fusion of transport vesicles to their acceptor membranes. RablB is essential for the intracellular transport of nascent low density lipoprotein (LDL) receptor. It is discussed as a universal mediator of endoplasmatic reticulum to Golgi transport of membrane glycoproteins in mammalian cells . The new protein can find clinical application m modulating the transport of glycoproteins inside cells, especially of the LDL receptor. Medline 96245776: Intracellular transport and maturation of nascent low density lipoprotein receptor is blocked by mutation in the Ras-related GTP-bmdmg protein, RAB1B strong similarity to rabl complete cDNA, complete eds, start at 47, EST hits Sequenced by Qiagen Locus : unknown Insert length: 1985 bp Poly A stretch at pos. 1901, polyadenylation signal at pos. 1859 1 GGGAGCAGAG TCGACTGGGA GCGACCGAGC GGGCCGCCGC CGCCGCCATG 51 AACCCCGAAT ATGACTACCT GTTTAAGCTG CTTTTGATTG GCGACTCAGG 101 CGTGGGCAAG TCATGCCTGC TCCTGCGGTT TGCTGATGAC ACGTACACAG 151 AGAGCTACAT CAGCACCATC GGGGTGGACT TCAAGATCCG AACCATCGAG 201 CTGGATGGCA AAACTATCAA ACTTCAGATC TGGGACACAG CGGGCCAGGA 251 ACGGTTCCGG ACCATCACTT CCAGCTACTA CCGGGGGGCT CATGGCATCA 301 TCGTGGTGTA TGACGTCACT GACCAGGAAT CCTACGCCAA CGTGAAGCAG 351 TGGCTGCAGG AGATTGACCG CTATGCCAGC GAGAACGTCA ATAAGCTCCT 401 GGTGGGCAAC AAGAGCGACC TCACCACCAA GAAGGTGGTG GACAACACCA 451 CAGCCAAGGA GTTTGCAGAC TCTCTGGGCA TCCCCTTCTT GGAGACGAGC 501 GCCAAGAATG CCACCAATGT CGAGCAGGCG TTCATGACCA TGGCTGCTGA 551 AATCAAAAAG CGGATGGGGC CTGGAGCAGC CTCTGGGGGC GAGCGGCCCA 601 ATCTCAAGAT CGACAGCACC CCTGTAAAGC CGGCTGGCGG TGGCTGTTGC 651 TAGGAGGGGC ACATGGAGTG GGACAGGAGG GGGCACCTTC TCCAGATGAT 701 GTCCCTGGAG GGGGGAGGAG GTACCTCCCT CTCCCTCTCC TGGGGCATTT 751 GAGTCTGTGG CTTTGGGGTG TCCTGGGCTC CCCATCTCCT TCTGGCCCAT 801 CTGCCTGCTG CCCTGAGCCC CGGTTCTGTC AGGGTCCCTA AGGGAGGACA 851 CTCAGGGCCT GTGGCCAGGC AGGGCGGAGG CCTGCTGTGC AGTTGCCTCT 901 AGGTGACTTT CCAAGATGCC CCCCTACACA CCTTTCTTTG GAACGAGGGC 951 TCTTCTGTCG GTGTCCCTCC CACCCCCATG TATGCTGCAC TGGGTTCTCT 1001 CCTTCTTCTT CCTGCTGTCC TGCCCAAGAA CTGAGGGTCT CCCCGGCCTC 1051 TACTGCCCTG GCTGCAGTCA GTGCCCAGGG CGAGGAATGT GGCCAGGGGA 1101 TCCAGGACCT GGGATCCAGG GCCCTGGGCT GGACCTCAGG ACAGGCATGG 1151 AGGCCACAGG GGCCCAGCAG CCCACCCTTT CCTCTCCCCA CTGCCTCCTC 1201 TCCCTTCCTA CACTCCCAGC TCGAGCCGTC CAGCTGCGGT GGGATCTGAG 1251 TATATCTAGG GCGGGTGGGC GGGTAGCAGT GCTGGGCCTG TGTCTTGAGC 1301 CTGGAGGGAG ACTGCTCCTG CCGCCCTCTG CCCTGCCGGA GACAGACCCA 1351 TGCGCTGCCT GCCCACCGTG CCCCTTTGTC CCCATGTCAG GCGGAGGCGG 1401 AAGGCCCACC GTGCCAGAGG CTGGGCACCA GCCTTAACCC TCACTCTGCT 1451 AGCACCTCCT CCCTTTCCCC AAGGTAGCAC ATCTGGCTCA CTCCCCACTC 1501 CGTCTCTGGA GCCCACCAGG GAAGGCCCTC ATCCCCTGCC GCTACTTCTC 1551 TGGGGAATGT GGGTTCCATC CAGGATTGGG GGCCTCTCTG CTCACCCACT 1601 CTGCACCCAG GATCCTAGTC CCCTGCCCTC TGGCACAGCT GCTTCCTGCA 1651 AGAAAGCAAG TCTTTGGTCT CCCTGAGAAG CCATGTCCCT CGTGCTGTCT 1701 CTTGCCTGTC CCACCTGTGC CCTGCCCTCC AGCTTGTATT TAAGTCCCTG 1751 GGCTGCCCCC TTGGGGTGCC CCCCGCTCCC AGGTTCCCCT CTGGTGTCAT 1801 GTCAGGCATT TTGCAAGGAA AAGCCACTTG GGGAAAGATG GAAAAGGACA 1851 AAAAAAATTA ATAAATTTCC ATTGGCCCTC GGGTGAGCTG AGGGTTTTTG 1901 CAAGGAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 1951 AAAAAAAAAA AAAAGAAAAA AAAAAAAAAA AAAAA BLAST Results No BLAST result Medline entries 91115900: A family of ras-like GTP-binding proteins expressed in electromotor neurons . Peptide information for frame 3 ORF from 48 bp to 650 bp; peptide length: 201 Category: strong similarity to known protein 1 MNPEYDYLFK LLLIGDSGVG KSCLLLRFAD DTYTESYIST IGVDFKIRTI 51 ELDGKTIKLQ IWDTAGQERF RTITSSYYRG AHGIIVVYDV TDQESYANVK 101 QWLQEIDRYA SENVNKLLVG NKSDLTTKKV VDNTTAKEFA DSLGIPFLET 151 SAKNATNVEQ AFMTMAAEIK KRMGPGAASG GERPNLKIDS TPVKPAGGGC 201 C BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_2ιl7, frame 3 SWISSPROT:RBlB_RAT RAS-RELATED PROTEIN RAB-1B., N = 1, Score = 1023, P = 2.7e-103 PIR:S06147 GTP-binding protein rablB - rat, N = 1, Score = 1013, P = 3.2e-102 SWISSPROT:RABl_DISOM RAS-RELATED PROTEIN ORAB-1. , N = 1, Score = 967, P = 2.4e-97 PIR:TVHUYP GTP-bind g protein Rabl - human, N = 1, Score = 966, P = 3e-97 >SWISΞPROT:RBlB_RAT RAS-RELATED PROTEIN RAB-1B. Length = 201 HSPs: Score = 1023 (153.5 bits), Expect = 2.7e-103, P = 2.7e-103 Identities = 197/201 (98%), Positives = 199/201 (99%) Query: 1 MNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQ 60 MNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQ Sbjct: 1 MNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYIΞTIGVDFKIRTIELDGKTIKLQ 60 Query: 61 IWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESYANVKQWLQEIDRYASENVNKLLVG 120 IWDTAGQERFRT+TSSYYRGAHGIIVVYDVTDQEΞYANVKQWLQEIDRYASENVNKLLVG Sbjct: 61 IWDTAGQERFRTVTSSYYRGAHGIIVVYDVTDQESYANVKQWLQEIDRYASENVNKLLVG 120 Query: 121 NKSDLTTKKVVDNTTAKEFADSLGIPFLETSAKNATNVEQAFMTMAAEIKKRMGPGAASG 180 NKSDLTTKKVVDNTTAKEFADSLG+PFLETSAKNATNVEQAFMTMAAEIKKRMGPGAASG Sbjct: 121 NKSDLTTKKVVDNTTAKEFADSLGVPFLETSAKNATNVEQAFMTMAAEIKKRMGPGAASG 180 Query: 181 GERPNLKIDSTPVKPAGGGCC 201 GERPNLKIDSTPVK A GGCC Sbjct: 181 GERPNLKIDSTPVKSASGGCC 201 Pedant information for DKFZphfbr2_2ιl7, frame 3 Report for DKFZphfbr2_2ιl7.3 [LENGTH] 201 [MW] 22171.25 [pi] 5.56 [HOMOL] SWISSPR0T:RB1B_RAT RAS-RELATED PROTEIN RAB-1B. le-112 [FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YFL038c] 2e-77 [FUNCAT] 30.08 organization of golgi [S. cerevisiae, YFL038c] 2e-77 [FUNCAT] 30.09 organization of intracellular transport vesicles [S. cerevisiae, YFL005w] 4e-57 [FUNCAT] 30.02 organization of plasma membrane [S. cerevisiae, YFL005w] 4e-57 [FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YFL005w] 4e-57 [FUNCAT] 08.19 cellular import [S. cerevisiae, YER031c] 8e-46 [FUNCAT] 08.13 vacuolar transport [S. cerevisiae, YER031c] 8e-46 [FUNCAT] 09.09 biogenesis of intracellular transport vesicles [S. cerevisiae, YGL210w] le-44 [FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YOR089c] le-30 [FUNCAT] 03.10 sporulation and germination [S. cerevisiae, YNL098c] 3e-25 [FUNCAT] 11.01 stress response [Ξ. cerevisiae, YNL098c] 3e-25 [FUNCAT] 03.99 other cell growth, cell division and dna synthesis activities [S. cerevisiae YNL098c] 3e-25 [FUNCAT] 01.03.13 regulation of nucleotide metabolism [Ξ. cerevisiae, YNL098C] 3e-25 [FUNCAT] 01.05.04 regulation of carbohydrate utilization [S. cerevisiae, YNL098c] 3e-25 [FUNCAT] 10.04.07 g-proteins [S. cerevisiae, YNL098c] 3e-25 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YNL098c] 3e-25 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YORlOlw] 9e-24 [FUNCAT] 11.10 cell death [S. cerevisiae, YORlOlw] 9e-24 [FUNCAT] 04.07 rna transport [S. cerevisiae, YOR185c] 4e-23 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YOR185c] 4e-23 [FUNCAT] 08.01 nuclear transport [S. cerevisiae, YOR185c] 4e-23 [FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YPR165w] 7e-17 [FUNCAT] 10.02.07 g-proteins [S. cerevisiae, YPR165w] le-11 [FUNCAT] 10.99 other signal-transduction activities [S. cerevisiae, YCR027c] le-16 [FUNCAT] 03.07 pheromone response, matmg-type determination, sex-specific proteins [S. cerevisiae, YLR229c] le-11 [FUNCAT] 10.05.07 g-proteins [S. cerevisiae, YLR229c] le-11 [FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YDL192w] 4e-10 [FUNCAT] 03.01 cell growth [S. cerevisiae, YNL180c] 9e-09 [FUNCAT] 06.07 protein modification (glycolsylation, acylation, myristylation, palmitylat on, farnesylation and processing) [S. cerevisiae, YPL051w] 3e-08 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YAL048c] 5e-05 [BLOCKS] BL01019A ADP-ribosylation factors family proteins [BLOCKS] BL01115A GTP-binding nuclear protein ran proteins [SCOP] dlplk 3.25.1.3.1 cH-p21 Ras protein [human (Homo sapiens) 2e-41 [SCOP] dlguaa_ 3.25.1.3.10 RaplA [Human (Homo sapiens) 5e-60 [SCOP] dlrrga_ 3.25.1.3.5 ADP-ribosylation factor 1 (ARFl) [rat (Rattu 2e-30 [SCOP] dlhura_ 3.25.1.3.4 ADP-ribosylation factor 1 (ARFl) [human (Horn 2e-33 [PIRKW] nucleus le-21 [PIRKW] membrane trafficking le-110 [PIRKW] oncogene le-25 [PIRKW] endoplasmic reticulum le-105 [PIRKW] phosphoprotein le-105 [PIRKW] glycoprotein 3e-25 [PIRKW] prenylated cysteine le-110 [PIRKW] signal transduction 4e-23 [PIRKW] transforming protein le-105 [PIRKW] purme nucleotide binding 2e-24 [PIRKW] alternative splicing 5e-26 [PIRKW] P-loop le-110 [PIRKW] lipoprotein le-110 [PIRKW] proto-oncogene 3e-27 [PIRKW] methylated carboxyl end 3e-27 [PIRKW] hydrolase 7e-25 [PIRKW] membrane protein le-105 [PIRKW] GTP binding le-110 [PIRKW] thiolester bond 5e-76 [PIRKW] Golgi apparatus le-105 [SUPFAM] ras transforming protein le-110 [PROSITE] ATP_GTP_A 1 [PROSITE] MYRISTYL 2 [PROSITE] CK2_PHOSPHO_SITE 5 [PROSITE] SIGMA54_INTERACT_1 1 [PROSITE] TYR_PHOSPHO_SITE 1 [PROSITE] GLYCOSAMINOGLYCAN 1 [PROSITE] PKC_PHOSPHO_SITE 4 [PROSITE] ASN_GLYCOSYLATION 3 [PFAM] Ras family (contains ATP/GTP binding P-loop) [KW] Alpha_Beta [KW] 3D SEQ MNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQ 22 lp- EEEEEEETTTTCHHHHHHHHHHCCCCCCCCCTTTEEEE-EEEEETTEEEEEE SEQ IWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESYANVKQWLQEIDRYASENVNKLLVG 22 lp- EEECTTTTTTCGGGHHHHHHCCEEEEEEETTBHHHHHHHHHHHHHHHHHHTTTTCEEEEE SEQ NKSDLTTKKVVDNTTAKEFADSLGIPFLETSAKNATNVEQAFMTMAAEIKKRMGPGAASG 22 lp- ETTTTCCC-CCCHHHHHHHHHHCCCCEEEETTTTTTTHHHHHHHHHHHHHH SEQ GERPNLKIDSTPVKPAGGGCC 221p- Prosite for DKFZphfbr2_2ιl7.3 PS00001 121->125 ASN_GLYCOSYLATION PDOC00001 PS00001 133->137 ASN_GLYCOSYLATION PDOC00001 PS00001 154->158 ASN__GLYCOSYLATION PDOC00001 PS00002 17->21 GLYCOSAMINOGLYCAN PDOC00002 PS00005 56->59 PKC_PHOSPHO_SITE PDOC00005 PS00005 126->129 PKC_PHOSPHO_SITE PDOC00005 PS00005 135->138 PKC_PHOSPHO_SITE PDOC00005 PS00005 151-M54 PKC_PHOSPHO_SITE PDOC00005 PS00006 32->36 CK2_PHOSPHO_SITE PDOC00006 PS00006 91->95 CK2_PHOSPHO_SITE PDOC00006 PS00006 135->139 CK2_PHOSPHO_SITE PDOC00006 PS00006 156->160 CK2_PHOSPHO_SITE PDOC00006 PS00006 179->183 CK2_PHOSPHO_SITE PDOC00006 PS00007 27->34 TYR_PHOSPHO_SITE PDOC00007 PS00008 18->24 MYRISTYL PDOC00008 PS00008 176->182 MYRISTYL PDOC00008 PS00017 15->23 ATP_GTP_A PDOC00017 PS00675 ll->25 SIGMA54 INTERACT 1 PDOC00579 Pfam for DKFZphfbr2_2ιl7.3 HMM_NAME Ras family (contains ATP/GTP binding P-loop) HMM *KLVLIGDSGVGKSCLLIRFTQNeFnEeYI PTIGvDFYtKTIEIDGKtIK KL+LIGDSGVGKSCLL+RF +++++E+YI+TIGVDF+++TIE+DGKTIK Query 10 KLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIK 58 HMM LQIWDTAGQERYRsMRPMYYRGAMGFMLVYDITNRqΞFENIrNWweEIrR LQIWDTAGQER+R+++++YYRGA+G+++VYD+T+++S+ N+++W++EI+R Query 59 LQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESYANVKQWLQEIDR 108 HMM HCDrDENVPIMLVGNKCDLEDQRQVStEEGQeFAREWGAIPFMETSAKTN +++ ENV ++LVGNK+DL +++V+ +++EFA+++G IPF+ETSAK++ Query 109 YAS—ENVNKLLVGNKSDLTTKKVVDNTTAKEFADSLG-IPFLETSAKNA 155 HMM iNVEEAFMEIvRellqrMqe.q.NqteNinidQpsrnrk... rCCCIM* +NVE+AFM+++ EI++RM+ +++E +N++ +S++ K +CC Query 156 TNVEQAFMTMAAEIKKRMGPGAASGGERPNLKIDSTPVKPAGGGCC-- 201 DKFZphfbr2_2kl9 group: brain derived DKFZphfbr2_2kl9 encodes a novel 303 amino acid protein with similarity to human KIAA0378 product . The protein contains a leucine zipper, which can mediate protem-protein-interaction. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can f nd application in studying the expression profile of brain-specific genes . similarity to KIAA0378 encoded by the genomic clones HS147M19/HS608E8 Sequenced by Qiagen Locus: unknown Insert length: 1931 bp Poly A stretch at pos. 1866, no polyadenylation signal found 1 GGGGGGGGCG CGCGGTGACA GCGCGGGGTT GGCGGCGTGG GACCCAGGGG 51 GCGACAGAGG CAGCAGCAGC CCGAGGCCTG AGGAGAGGAG ACCGGCGGCG 101 GCGGCAATGC TGGAGACCCT TCGCGAGCGG CTGCTGAGCG TGCAGCAGGA 151 TTTCACCTCC GGGCTGAAGA CTTTAAGTGA CAAGTCAAGA GAAGCAAAAG 201 TGAAAAGCAA ACCCAGGACT GTTCCATTTT TGCCAAAGTA CTCTGCTGGA 251 TTAGAATTAC TTAGCAGGTA TGAGGATACA TGGGCTGCAC TTCACAGAAG 301 AGCCAAAGAC TGTGCAAGTG CTGGAGAGCT GGTGGATAGC GAGGTGGTCA 351 TGCTTTCTGC GCACTGGGAG AAGAAAAAGA CAAGCCTCGT GGAGCTGCAA 401 GAGCAGCTCC AGCAGCTCCC AGCTTTAATC GCAGACTTAG AATCCATGAC 451 AGCAAATCTG ACTCATTTAG AGGCGAGTTT TGAGGAGGTA GAGAACAACC 501 TGCTGCATCT GGAAGACTTA TGTGGGCAGT GTGAATTAGA AAGATGCAAA 551 CATATGCAGT CCCAGCAACT GGAGAATTAC AAGAAAAATA AGAGGAAGGA 601 ACTTGAAACC TTCAAAGCTG AACTAGATGC AGAGCACGCC CAGAAGGTCC 651 TGGAAATGGA GCACACCCAG CAAATGAAGC TGAAGGAGCG GCAGAAGTTT 701 TTTGAGGAAG CCTTCCAGCA GGACATGGAG CAGTACCTGT CCACTGGCTA 751 CCTGCAGATT GCAGAGCGGC GAGAGCCCAT AGGCAGCATG TCATCCATGG 801 AAGTGAACGT GGACATGCTG GAGCAGATGG TCCTGATGGA CATATCGGAC 851 CAGGAGGCCC TGGACGTCTT CCTGAACTCT GGAGGAGAAG AGAACACTGT 901 GCTGTCCCCC GCCTTAGGTA GGGTTGACAA ACTTGCATTA GCTGAACCAG 951 GGCAGTATCG ATGCCACTCC CCTCCAAAGG TGAGACGTGA GAACCATCTG 1001 CCAGTCACTT ACGCATAAAC CCCCAAGCTC ACAGCCAGCT CCTGGCTCCC 1051 TAACCCCACG GTTCCACACG GCTGTGTGGC AGCTGCAACA GTGGTGTGGT 1101 TCCGTCATGA ATTCTTCTCA AAGATTTGAC ATGCTCCACT CCGGTAACTT 1151 TGGTGAGTTG AGAGCTTTCT TGTTTGTTTT CCCTCCTTTA CCATCCAGAA 1201 ATCCATTTGA GTCTGCTCCT TGTGGTTAAG GACTGGCGTT TGCAGGGAGG 1251 TGCGGACTCT CCTGCGGGGC TCACGGGAAA CTCTTCCCTC TTCGTGCGAC 1301 AGGCATTTAG GGGCGTGCCT GCCATGGGCA AAGCCATGGT GTGTGTTCAG 1351 CTCTTGGCCT GTGTTGTAAA CTTAGTTGCA CTTCAGTTCC TTTCATCCCT 1401 TCACAAAATT TTGTTTCACA TTCATGCAGC AAATATGGGC TGAGGTGCCA 1451 GACCTGTACC TGGGCTTGGT GCGTTTCAAA TTTCAGACCA GTTCTTTGGG 1501 CTGGGTCAAG GCAAAGCTCA GTCGTCCCAG CAGCACCTCA GCCATCTGTA 1551 GAAGGTTCTA CCATTACCAC GGTTTCAGCT TCCTCTAAAC TTCTCACCCG 1601 CTTCTCCTGG CAATCTGTCA GAACGGTGTC ATCCTGGGGA AGAGAAGGAG 1651 CTTGGGTGCA TTTGCCCTCA TCCTGAGAAG GCCAGAATAC TGGAGACCAG 1701 CGTGAACCCT CACCCAGAGT CAGGGGAAGA TTTAGAAACA GTGACACCTG 1751 CATATAGAAT TTTGATTCCT TGAAGAGCCT ATTTAGTTCC ATAAAATTGG 1801 AGAACTGCTG AAGGTCAGTA ATTCCGACTT TCTCAGCAGT GGTGTCTCTG 1851 AATTACTGCA AAGGGTAAAA AAAAAAAAAA AAAAAACTTA TCGATACCGT 1901 CGACCTCGAT GATGATGATG ATGATGTCGA C BLAST Results Entry HS147M19 from database EMBL: Homo sapiens DNA sequence from PAC 147M19 on chromosome 6p22.1-22.3. Contains an unknown gene, ESTs and GSSs. Score = 5540, P = 4.1e-275, identities = 1114/1120 3 exons 592-1884 Entry HS608E8 from database EMBL: Human DNA sequence *** SEQUENCING IN PROGRESS *** from clone 608E8 Score = 797, P = 1.2e-78, identities = 161/163 6 exons 1-592 Medline entries 90294724: The involucrin gene of the gibbon: The middle region shared by the hominoids Peptide information for frame 2 ORF from 107 bp to 1015 bp; peptide length: 303 Category: similarity to known protein Classification: unset Prosite motifs: LEUCINE ZIPPER (97-119) 1 MLETLRERLL SVQQDFTSGL KTLSDKSREA KVKSKPRTVP FLPKYSAGLE 51 LLSRYEDTWA ALHRRAKDCA SAGELVDSEV VMLSAHWEKK KTSLVELQEQ 101 LQQLPALIAD LESMTANLTH LEASFEEVEN NLLHLEDLCG QCELERCKHM 151 QSQQLENYKK NKRKELETFK AELDAEHAQK VLEMEHTQQM KLKERQKFFE 201 EAFQQDMEQY LSTGYLQIAE RREPIGSMSS MEVNVDMLEQ MVLMDISDQE 251 ALDVFLNSGG EENTVLSPAL GRVDKLALAE PGQYRCHSPP KVRRENHLPV 301 TYA BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_2kl9, frame 2 TREMBL :HSAB2376_1 gene: "KIAA0378"; Human mRNA for KIAA0378 gene, partial eds., N = 1, Score = 137, P = 4.8e-06 PIR: 137037 involucrin - common gibbon, N = 1, Score = 124, P = 7.4e-05 PIR:A57013 early endosome antigen 1 - human, N = 1, Score = 128, P = 9.5e-05 >TREMBL:HSAB2376_1 gene: "KIAA0378"; Human mRNA for KIAA0378 gene, partial eds . Length = 808 HSPs: Score = 137 (20.6 bits), Expect = 4.8e-06, P = 4.8e-06 Identities = 59/222 (26%), Positives = 103/222 (46%) Query: 2 LETLRERLLΞVQQDFTSGLKTL SDKSREAKVKS-KPRTVPFLPKYSAGLELLSRYED 57 L TL E L S ++ LK D+ R +++S + K +A L+ E Sbjct: 434 LATLEEAL-SEKERIIERLKEQRERDDRERLEEIESFRKENKDLKEKVNALQAELTEKES 492 Query: 58 TWAALHRRAKDCASAGELVDSEVVMLSAHWEKKKTSLVELQEQLQQLPALIADLESMTAN 117 + L A ASAG DS++ L E+KK +L+ QL++ I D M Sbjct: 493 SLIDLKEHASSLASAGLKRDSKLKSLEIAIEQKKEECSKLEAQLKKAHN-IEDDSRMNPE 551 Query: 118 LTHLEASFEEVENNLLHLEDLCG—QCELERCKHMQSQQLENYKKNKRK ELETFKAE 172 ++++ + D CG Q E++R + +++EN K +K K ELE+ Sbjct: 552 FAD QIKQLDKEASYYRDECGKAQAEVDRLLEIL-KEVENEKNDKDKKIAELESLTLR 607 Query: 173 LDAEHAQKVLEMEHTQQMKLKERQKFFEEAFQQDMEQYLSTGYLQIAE 220 + +KV ++H QQ++ K+ + EE +++ ++ +LQI E Sbjct: 608 HMKDQNKKVANLKHNQQLEKKKNAQLLEEVRRREDSMADNSQHLQIEE 655 Score = 100 (15.0 bits), Expect = 6.2e-02, P = 6.0e-02 Identities = 44/156 (28%), Positives = 76/156 (48%) Query: 57 DTWAALHRRAKDCASAGELVDSEVVMLSAHWEKKKTSLVELQEQLQQLPAL-IADLESMT 115 D A+ +R +C A VD + +L E +K + +L+ L + D Sbjct: 560 DKEASYYR—DECGKAQAEVDRLLEILK-EVENEKNDKDKKIAELESLTLRHMKDQNKKV 616 Query: 116 ANLTHLEASFEEVENNLLHLEDLCGQCE—LERCKHMQSQQLENYKKNKRKELETFKAEL 173 ANL H + E+ +N L LE++ + + + +H+Q ++L N + R+EL+ KA L Sbjct: 617 ANLKHNQ-QLEKKKNAQL-LEEVRRREDSMADNSQHLQIEELMNALEKTRQELDATKARL 674 Query: 174 DAEHAQKVLEME-HTQQMKLKERQKFFEEAFQQDMEQYLS 212 A Q + E E H +++ ER+K EE + E L+ Sbjct: 675 -ASTQQSLAEKEAHLANLRI-ERRKQLEEILEMKQEALLA 712 Pedant information for DKFZphfbr2_2kl9, frame 2 Report for DKFZphfbr2_2kl9.2 [LENGTH] 303 [MW] 34814.78 [pi] 5.23 [PROSITE] LEUCINE ZIPPER 1 [KW] All Alpha [KW] LOW COMPLEXITY 3.63 % [KW] COILED COIL 14.52 % SEQ MLETLRERLLSVQQDFTSGLKTLSDKSREAKVKΞKPRTVPFLPKYSAGLELLSRYEDTWA SEG PRD ccchhhhhhhhhhhccccccchhhhhhhhhhhhccccccccccchhhhhhhhhhhhchhh COILS SEQ ALHRRAKDCASAGELVDSEVVMLSAHWEKKKTSLVELQEQLQQLPALIADLESMTANLTH SEG xxxxxxxxxxx PRD hhhhhhhhchhhhhcccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCC SEQ LEASFEEVENNLLHLEDLCGQCELERCKHMQSQQLENYKKNKRKELETFKAELDAEHAQK SEG PRD hhhhhhhhhhhhhhhcccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCC SEQ VLEMEHTQQMKLKERQKFFEEAFQQDMEQYLSTGYLQIAERREPIGSMSSMEVNVDMLEQ SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcchhhhhhhcccccccccchhhhhhhhh COILS SEQ MVLMDISDQEALDVFLNSGGEENTVLSPALGRVDKLALAEPGQYRCHSPPKVRRENHLPV SEG PRD hhhhhhchhhhhhhhhccccccceeeccccccccceeeccccccccccccceeecccccc COILS SEQ TYA SEG PRD COILS Prosite for DKFZphfbr2_2kl9.2 PS00029 97->119 LEUCINE ZIPPER PDOC00029 (No Pfam data available for DKFZphfbr2_2kl9.2) DKFZphfbr2_2kl4 group: cell cycle DKFZphfbr2_2kl4 encodes a novel 335 ammo acid protein with strong similarity to rattus rattus IAG2 "implantation-associated protein" and the human N33 tumour-suppressor gene. Tumour-suppressor genes are known to be involved m the control of cell growth and division, interacting with proteins which control the cell cycle. The N33 gene is significantly methylated in tumour cells, a mechanism by which tumor-suppressor genes are inactivated in cancer. In addition, the novel protein contains a RGD cell attachment site. Therefore the novel protein is a new putative tumour-suppressor gene. The new protein can find application in modulating/blocking the cell cycle and in the therapy of tumours. strong similarity to human N33 tumor suppressor gene complete cDNA, complete eds, EST hits, potential start at Bp 30 matches kozak consensus ANCatgG potential transmembran protein (4 TM) similarity to yeast OST3p (oligosaccharyltransferase gamma chain) Sequenced by Qiagen Locus: unknown Insert length: 2241 bp Poly A stretch at pos. 2221, no polyadenylation signal found 1 TGGGACTTAT AGAAGGGAGA GGAGCGAACA TGGCAGCGCG TTGGCGGTTT 51 TGGTGTGTCT CTGTGACCAT GGTGGTGGCG CTGCTCATCG TTTGCGACGT 101 TCCCTCAGCC TCTGCCCAAA GAAAGAAGGA GATGGTGTTA TCAGAAAAGG 151 TTAGTCAGCT GATGGAATGG ACTAACAAAA GACCTGTAAT AAGAATGAAT 201 GGAGACAAGT TCCGTCGCCT TGTGAAAGCC CCACCGAGAA ATTACTCCGT 251 TATCGTCATG TTCACTGCTC TCCAACTGCA TAGACAGTGT GTCGTTTGCA 301 AGCAAGCTGA TGAAGAATTC CAGATCCTGG CAAACTCCTG GCGATACTCC 351 AGTGCATTCA CCAACAGGAT ATTTTTTGCC ATGGTGGATT TTGATGAAGG 401 CTCTGATGTA TTTCAGATGC TAAACATGAA TTCAGCTCCA ACTTTCATCA 451 ACTTTCCTGC AAAAGGGAAA CCCAAACGGG GTGATACATA TGAGTTACAG 501 GTGCGGGGTT TTTCAGCTGA GCAGATTGCC CGGTGGATCG CCGACAGAAC 551 TGATGTCAAT ATTAGAGTGA TTAGACCCCC AAATTATGCT GGTCCCCTTA 601 TGTTGGGATT GCTTTTGGCT GTTATTGGTG GACTTGTGTA TCTTCGAAGA 651 AGTAATATGG AATTTCTCTT TAATAAAACT GGATGGGCTT TTGCAGCTTT 701 GTGTTTTGTG CTTGCTATGA CATCTGGTCA AATGTGGAAC CATATAAGAG 751 GACCACCATA TGCCCATAAG AATCCCCACA CGGGACATGT GAATTATATC 801 CATGGAAGCA GTCAAGCCCA GTTTGTAGCT GAAACACACA TTGTTCTTCT 851 GTTTAATGGT GGAGTTACCT TAGGAATGGT GCTTTTGTGT GAAGCTGCTA 901 CCTCTGACAT GGATATTGGA AAGCGAAAGA TAATGTGTGT GGCTGGTATT 951 GGACTTGTTG TATTATTCTT CAGTTGGATG CTCTCTATTT TTAGATCTAA 1001 ATATCATGGC TACCCATACA GCTTTCTGAT GAGTTAAAAA GGTCCCAGAG 1051 ATATATAGAC ACTGGAGTAC TGGAAATTGA AAAACGAAAA TCGTGTGTGT 1101 TTGAAAAGAA GAATGCAACT TGTATATTCT GTATTACCTC TTTTTTTCAA 1151 GTGATTTAAA TAGTTAATCA TTTAACCAAA GAAGATGTGT AGTGCCTTAA 1201 CAAGCAATCC TCTGTCAAAA TCTGAGGTAT TTGAAAATAA TTATCCTCTT 1251 AACCTTCTCT TCCCAGTGAA CTTTATGGAA CATTTAATTT AGTACAATTA 1301 AGTATATTAT AAAAATTGTA AAACTACTAC TTTGTTTTAG TTAGAACAAA 1351 GCTCAAAACT ACTTTAGTTA ACTTGGTCAT CTGATCTTAT ATTGCCTTAT 1401 CCAAAGATGG GGAAAGTAAG TCCTGACCAG GTGTTCCCAC ATATGCCTGT 1451 TACAGATAAC TACATTAGGA ATTCATTCTT AGCTTCTTCA TCTTTGTGTG 1501 GATGTGTATA CTTTACGCAT CTTTCCTTTT GAGTAGAGAA ATTATGTGTG 1551 TCATGTGGTC TTCTGAAAAT GGAACACCAT TCTTCAGAGC ACACGTCTAG 1601 CCCTCAGCAA GACAGTTGTT TCTCCTCCTC CTTGCATATT TCCTACTGCG 1651 CTCCAGCCTG AGTGATAGAG TGAGACTCTG TCTCAAAAAA AAAGTATCTC 1701 TAAATACAGG ATTATAATTT CTGCTTGAGT ATGGTGTTAA CTACCTTGTA 1751 TTTAGAAAGA TTTCAGATTC ATTCCATCTC CTTAGTTTTC TTTTAAGGTG 1801 ACCCATCTGT GATAAAAATA TAGCTTAGTG CTAAAATCAG TGTAACTTAT 1851 ACATGGCCTA AAATGTTTCT ACAAATTAGA GTTTGTCACT TATTCCATTT 1901 GTACCTAAGA GAAAAATAGG CTCAGTTAGA AAAGGACTCC CTGGCCAGGC 1951 GCAGTGACTT ACGCCTGTAA TCTCAGCACT TTGGGAGGCC AAGGCAGGCA 2001 GATCACGAGG TCAGGAGTTC GAGACCATCC TGGCCAACAT GGTGAAACCC 2051 CGTCTCTACT AAAAATATAA AAATTAGCTG GGTGTGGTGG CAGGAGCCTG 2101 TAATCCCAGC TGCACAGGAG GCTGAGGCAC GAGAATCACT TGAACTCAGG 2151 AGATGGAGGT TTCAGTGAGC CGAGATCACG CCACTGCACT CCAGCCTGGC 2201 AACAGAGCGA GACTCCATCT CAAAAAAAAA AAAAAAAAAA A BLAST Results No BLAST result Medline entries 96299740: Structure and methylation-associated silencing of a gene within a homozygously deleted region of human chromosome band 8p22. 97243398: Tumour-suppressor genes in prostatic oncogenesis: a positional approach. 98334474: Concordant methylation of the ER and N33 genes in glioblastoma multiforme. Peptide information for frame 3 ORF from 30 bp to 1034 bp; peptide length: 335 Category: strong similarity to known protein 1 MAARWRFWCV SVTMVVALLI VCDVPSASAQ RKKEMVLSEK VSQLMEWTNK 51 RPVIRMNGDK FRRLVKAPPR NYSVIVMFTA LQLHRQCVVC KQADEEFQIL 101 ANSWRYSSAF TNRIFFAMVD FDEGSDVFQM LNMNSAPTFI NFPAKGKPKR 151 GDTYELQVRG FSAEQIARWI ADRTDVNIRV IRPPNYAGPL MLGLLLAVIG 201 GLVYLRRSNM EFLFNKTGWA FAALCFVLAM TSGQMWNHIR GPPYAHKNPH 251 TGHVNYIHGS SQAQFVAETH IVLLFNGGVT LGMVLLCEAA TSDMDIGKRK 301 IMCVAGIGLV VLFFSWMLSI FRSKYHGYPY SFLMS BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_2kl4, frame 3 TREMBL:RNAF855 _1 gene: "IAG2"; product: "implantation-associated protein"; Rattus norvegicus implantation-associated protein (IAG2) mRNA, partial eds., N = 1, Score = 1560, P = 3.4e-160 PIR:G02297 gene N33 protein - human, N = 1, Score = 1256, P = 5.6e-128 TREMBL:HSN33S11_1 gene: "N33"; product: "N33 protein form 2"; Human N33 protein form 2 (N33) gene, exon 11 and complete eds., N = 1, Score = 1252, P = 1.5e-127 >TREMBL:RNAF8554_1 gene: "IAG2"; product: "implantation-associated protein"; Rattus norvegicus implantation-associated protein (IAG2) mRNA, partial eds. Length = 308 HSPs: Score = 1560 (234.1 bits), Expect = 3.4e-160, P = 3.4e-160 Identities = 295/307 (96%), Positives = 299/307 (97%) Query: 29 AQRKKEMVLSEKVSQLMEWTNKRPVIRMNGDKFRRLVKAPPRNYSVIVMFTALQLHRQCV 88 AQRKKE VL EKV QLMEWTN+RPVIRMNGDKFR LVKAPPRNYSVIVMFTALQLHRQCV Sbjct: 2 AQRKKEKVLVEKVIQLMEWTNQRPVIRMNGDKFRPLVKAPPRNYSVIVMFTALQLHRQCV 61 Query: 89 VCKQADEEFQILANSWRYSSAFTNRIFFAMVDFDEGSDVFQMLNMNΞAPTFINFPAKGKP 148 VCKQADEEFQILAN WRYSSAFTNRIFFAMVDFDEGSDVFQMLNMNSAPTFINFP KGKP Sbjct: 62 VCKQADEEFQILANFWRYSSAFTNRIFFAMVDFDEGSDVFQMLNMNSAPTFINFPPKGKP 121 Query: 149 KRGDTYELQVRGFSAEQIARWIADRTDVNIRVIRPPNYAGPLMLGLLLAVIGGLVYLRRS 208 KR DTYELQVRGFSAEQIARWIADRTDVNIRVIRPPNYAGPLMLGLLLAVIGGLVYLRRS Sbjct: 122 KRADTYELQVRGFSAEQIARWIADRTDVNIRVIRPPNYAGPLMLGLLLAVIGGLVYLRRS 181 Query: 209 NMEFLFNKTGWAFAALCFVLAMTSGQMWNHIRGPPYAHKNPHTGHVNYIHGSSQAQFVAE 268 NMEFLFNKTGWAFAALCFVLAMTSGQMWNHIRGPPYAHKNPHTGHVNYIHGSSQAQFVAE Sbjct: 182 NMEFLFNKTGWAFAALCFVLAMTSGQMWNHIRGPPYAHKNPHTGHVNYIHGSSQAQFVAE 241 Query: 269 THIVLLFNGGVTLGMVLLCEAATSDMDIGKRKIMCVAGIGLVVLFFSWMLSIFRSKYHGY 328 THIVLLFNGGVTLGMVLLCEAA ΞDMDIGKR++MC+AGIGLVVLFFSWMLSIFRSKYHGY Sb ct: 242 THIVLLFNGGVTLGMVLLCEAAASDMDIGKRRMMCIAGIGLVVLFFSWMLSIFRSKYHGY 301 Query: 329 PYSFLMS 335 PYSFLMS Sbjct: 302 PYSFLMS 308 Pedant information for DKFZphfbr2_2kl4, frame 3 Report for DKFZphfbr2_2kl4.3 [LENGTH 335 [MW] 38036.83 [pi] 9.68 [HOMOL] TREMBL:RNAF8554_1 gene: "IAG2"; product: "implantation-associated protein"; Rattus norvegicus implantation-associated protein (IAG2) mRNA, partial eds. le-161 [FUNCAT] 30.07 organization of endoplasmatic reticulum [S. cerevisiae, YOR085w] 4e-14 [FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylation, farnesylation and processing) [S. cerevisiae, YOR085w] 4e-14 [FUNCAT] 01.05.01 carbohydrate utilization [S. cerevisiae, YOR085w] 4e-14 [EC] 2.4.1.119 Dolιchyl-dιphosphoolιgosacchaπde--proteιn glycosyltransferase le-12 [PIRKW] glycosyltransferase le-12 [PIRKW] transmembrane protein 6e-69 [PIRKW] hexosyltransferase le-12 [PROSITE] RGD 1 [PROSITE] MYRISTYL 4 [PROSITE] AMIDATION 1 [PROSITE] CK2_PH0SPH0_SITE 2 [PROSITE] PKC_PHOSPHO_SITE 4 [PROSITE] ASN_GLYCOSYLATION 2 [KW] SIGNAL_PEPTIDE 30 [KW] TRANSMEMBRANE 4 [KW] LOW COMPLEXITY 5.97 % SEQ MAARWRFWCVSVTMVVALLIVCDVPSASAQRKKEMVLSEKVSQLMEWTNKRPVIRMNGDK SEG PRD cccceeeeeeehhhhhhhhhhhccccccchhhhhhhhhhhhhhhhhhhccceeeeecccc MEM SEQ FRRLVKAPPRNYSVIVMFTALQLHRQCVVCKQADEEFQILANSWRYSSAFTNRIFFAMVD SEG PRD ceeeeeccccccceeeehhhhhhccceeeehhhhhhhhhhhhhcccccccccceeeeeec MEM SEQ FDEGSDVFQMLNMNSAPTFINFPAKGKPKRGDTYELQVRGFSAEQIARWIADRTDVNIRV SEG PRD cccccceeeecccccccceeeccccccccccceeeeeeeccchhhhhhhhhhhhheeeee MEM M SEQ IRPPNYAGPLMLGLLLAVIGGLVYLRRSNMEFLFNKTGWAFAALCFVLAMTSGQMWNHIR SEG xxxxxxxxxxxxxxxxxxxx PRD eccccccchhhhhhhhhhhcchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccceeec MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMM MMMMMMMMMMMMMMMMMMMMMMMM ... SEQ GPPYAHKNPHTGHVNYIHGSSQAQFVAETHIVLLFNGGVTLGMVLLCEAATSDMDIGKRK SEG PRD ccccccccccccceeeecccchhhhhhhheeeeeeccchhhhhhhhhhhhcccccccccc MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMM SEQ IMCVAGIGLVVLFFSWMLSIFRSKYHGYPYSFLMS SEG PRD eeeecccceeeeeehhhhhhhhhhccccccccccc MEM MMMMMMMMMMMMMMMMMMMMMMMMMM Prosite for DKFZphfbr2 2kl4.3 PS00001 71->75 ASN_GLYCOSYLATION PDOC00001 PS00001 215->219 ASN_GLYCOΞYLATION PDOC00001 PS00005 38->41 PKC_PHOSPHO_SITE PDOC00005 PS00005 48->51 PKC PHOSPHO SITE PDOC00005 PS00005 103-->106 PKC PHOSPHO SITE PDOC00005 PS00005 111- ->114 PKC PHOSPHO " SITE PDOC00005 PS00006 208- ->212 CK2 PHOSPHO " " SITE PDOC00006 PS00006 292- ->296 CK2 PHOSPHO- " SITE PDOC00006 PS00008 193- ->199 MYRISTYL PDOC00008 PS00008 233- ->239 MYRISTYL PDOC00008 PS00008 259- ->265 MYRISTYL PDOC00008 PS00008 278- ->284 MYRISTYL PDOC00008 PS00009 296- ->300 AMIDATION PDOC00009 PS00016 150- ->153 RGD PDOC00016 (No Pfam data available for DKFZphfbr2_2kl4.3) DKFZphfbr2_3cl8 group: nucleic acid management DKFZphfbr2_3cl8 encodes a novel 448 ammo acid protein with strong similarity to mus musculus RNA helicase and several RNA-dependent ATPases from the DEAD box family. RNA helicases comprise a large family of proteins that are involved in basic biological systems such as nuclear and mitochondrial splicing processes, RNA editing, rRNA processing, translation initiation, nuclear mRNA export, and mRNA degradation. RNA helicases are essential factors in cell development and differentiation, and some of them play a role m transcription and replication of viral single-stranded RNA genomes. The members of the largest subgroup, the DEAD and DEAH box proteins, exhibit a strong dependence of the unwinding activity on ATP hydrolysis. The novel protein contains a DEAD-box and is a new member of this subgroup. The new protein can find application in modulating RNA metabolism and gene expression. strong similarity to RNA helicase and RNA-dependent ATPase from the DEAD box family group helicases Summary DKFZphfbr2_3cl8 encodes a novel 448 ammo acid protein with similarity to DEAD-box subfamily ATP-dependent RNA helicases. Deletion of the yeast homolouge DBP5 is lethal. strong similarity to RNA helicase and RNA-dependent ATPase from the DEAD box family complete cDNA, EST hits complete eds ATG at Bp 109 Sequenced by AGOWA Locus: /map="87.50 cR from top of Chrl6 linkage group" Insert length: 1713 bp Poly A stretch at pos. 1696, no polyadenylation signal found 1 TGGGGTAGTG GGGCTGGAGC AGAGCCTGCC GCGAACCCCC GGAGCCCACG 51 ATCCCTCGTG CCATCCCTCG AATCCACCAG CACGAGCGTC CCACCCGCGC 101 CTGGGACCAT GGCCACTGAC TCATGGGCCC TGGCGGTGGA CGAGCAGGAA 151 GCTGCGGCTG AGTCGTTGAG CAACTTGCAT CTTAAGGAAG AGAAAATCAA 201 ACCAGATACC AATGGTGCTG TTGTCAAGAC CAATGCCAAT GCAGAGAAGA 251 CAGATGAAGA AGAGAAAGAG GACAGAGCTG CCCAGTCCTT ACTCAACAAG 301 CTGATCAGAA GCAACCTTGT TGATAACACA AACCAAGTGG AAGTCCTGCA 351 GCGGGATCCA AACTCCCCTC TGTACTCGGT GAAGTCTTTT GAAGAGCTTC 401 GGCTCCCACA GAACTTAATT GCCCAATCTC AGTCTGGTAC TGGTAAAACA 451 GCTGCCTTCG TGCTGGCCAT GCTTAGCCAA GTAGAACCTG CAAACAAATA 501 CCCCCAGTGT CTATGTCTCT CCCCAACGTA TGAGCTCGCC CTCCAAACAG 551 GAAAAGTGAT TGAACAAATG GGCAAATTTT ACCCTGAACT GAAGCTAGCT 601 TATGCTGTTC GAGGCAATAA ATTGGAAAGA GGCCAGAAGA TCAGTGAGCA 651 GATTGTCATT GGCACCCCTG GGACTGTGCT GGACTGGTGC TCCAAGCTCA 701 AGTTCATTGA TCCCAAGAAA ATCAAGGTGT TTGTTCTGGA TGAGGCTGAT 751 GTCATGATAG CCACTCAGGG CCACCAAGAT CAGAGCATCC GCATCCAGAG 801 GATGCTGCCC AGGAACTGCC AGATGCTGCT TTTCTCCGCC ACCTTTGAAG 851 ACTCTGTGTG GAAGTTTGCC CAGAAAGTGG TCCCAGACCC AAACGTTATC 901 AAACTGAAGC GTGAGGAAGA GACCCTGGAC ACCATCAAGC AGTACTATGT 951 CCTGTGCAGC AGCAGAGACG AGAAGTTCCA GGCCTTGTGT AACCTCTACG 1001 GGGCCATCAC CATTGCTCAA GCCATGATCT TCTGCCATAC TCGCAAAACA 1051 GCTAGTTGGC TGGCAGCAGA GCTCTCAAAA GAAGGCCACC AGGTGGCTCT 1101 GCTGAGTGGG GAGATGATGG TGGAACAGAG GGCTGCAGTG ATTGAGCGCT 1151 TCCGAGAGGG CAAAGAGAAG GTTTTGGTGA CCACCAACGT GTGTGCCCGC 1201 GGCATTGATG TTGAACAAGT GTCTGTCGTC ATCAACTTTG ATCTTCCCGT 1251 GGACAAGGAC GGGAATCCTG ACAATGAGAC CTACCTGCAC CGGATCGGGC 1301 GCACGGGCCG CTTTGGCAAG AGGGGCCTGG CAGTGAACAT GGTGGACAGC 1351 AAGCACAGCA TGAACATCCT GAACAGAATC CAGGAGCATT TTAATAAGAA 1401 GATAGAAAGA TTGGACACAG ATGATTTGGA CGAGATTGAG AAAATAGCCA 1451 ACTGAGAAGC TCCACCAGCC ACTGATGCCA GCCCTGGCAC TGCCCCTGCA 1501 CAGGAGACAA GTGCGTTCAG GGCACAGGCC CCGACATCAC CCCAAGGACA 1551 ACGGCACAAG TAGAGAGAAA CTACCTACCT CACTTCAAAT TATGTTTGGA 1601 CTTGACAAAA ATGTATGCAA ATGATGGGGG ATGGTAGAAA AAAATTATTT 1651 ACACAACCTT GGAAGATTAG GCATGAATAC ACAGAGATTT ACCTTTAAAA 1701 AAAAAAAAAA AAA BLAST Results Entry G36496 from database EMBL: SHGC-53094 Human Homo sapiens STS cDNA. Length = 459 Minus Strand HSPs: Score = 1693 (254.0 bits), Expect = 2.8e-70, P = 2.8e-70 Identities = 369/387 (95%), Positives = 369/387 (95%) Entry G44014 from database EMBLNEW: WIAF-3643-STS Human THudson SANGER Homo sapiens STS genomic, sequence tagged site. Score = 901, P = 2.3e-35, identities = 183/185 Medline entries 94192995: Gene 1994 Mar 25; 140 (2) : 171-177 Mouse erythroid cells express multiple putative RNA helicase genes exhibiting high sequence conservation from yeast to mammals. Peptide information for frame 1 ORF from 109 bp to 1452 bp; peptide length: 446 Category: strong similarity to known protein 1 MATDSWALAV DEQEAAAESL SNLHLKEEKI KPDTNGAVVK TNANAEKTDE 51 EEKEDRAAQS LLNKLIRSNL VDNTNQVEVL QRDPNSPLYS VKSFEELRLP 101 QNLIAQSQSG TGKTAAFVLA MLSQVEPANK YPQCLCLSPT YELALQTGKV 151 IEQMGKFYPE LKLAYAVRGN KLERGQKISE QIVIGTPGTV LDWCSKLKFI 201 DPKKIKVFVL DEADVMIATQ GHQDQSIRIQ RMLPRNCQML LFSATFEDSV 251 WKFAQKVVPD PNVIKLKREE ETLDTIKQYY VLCSSRDEKF QALCNLYGAI 301 TIAQAMIFCH TRKTASWLAA ELSKEGHQVA LLSGEMMVEQ RAAVIERFRE 351 GKEKVLVTTN VCARGIDVEQ VSVVINFDLP VDKDGNPDNE TYLHRIGRTG 401 RFGKRGLAVN MVDSKHSMNI LNRIQEHFNK KIERLDTDDL DEIEKIAN BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_3cl8, frame 1 PIR: 149731 RNA helicase - mouse, N = 2, Score = 1758, P = 3.8e-223 TREMBL :AF005239_1 gene: "Dbp80"; product: "DEAD-box helicase"; Drosophila melanogaster DEAD-box helicase (DbpδO) mRNA, complete eds., N = 2, Score = 1142, P = 1.8e-125 SWISSPROT:YB66_SCHPO PUTATIVE ATP-DEPENDENT RNA HELICASE C12C2.06., N = 2, Score = 911, P = 5.5e-103 PIR:S66920 probable RNA helicase CA5/6 - yeast (Saccharomyces cerevisiae), N = 2, Score = 887, P = 1.9e-98 >PIR:I49731 RNA helicase - mouse Length = 478 HSPs: Score = 1758 (263.8 bits), Expect = 3.8e-223, Sum P(2) = 3.8e-223 Identities = 338/349 (96%), Positives = 349/349 (100%) Query: 100 PQNLIAQSQSGTGKTAAFVLAMLSQVEPANKYPQCLCLSPTYELALQTGKVIEQMGKFYP 159 PQNLIAQSQSGTGKTAAFVLAMLS+VEPA++YPQCLCLSPTYELALQTGKVIEQMGKF+P Sbjct: 130 PQNLIAQSQSGTGKTAAFVLAMLSRVEPADRYPQCLCLSPTYELALQTGKVIEQMGKFHP 189 Query: 160 ELKLAYAVRGNKLERGQKISEQIVIGTPGTVLDWCSKLKFIDPKKIKVFVLDEADVMIAT 219 ELKLAYAVRGNKLERGQK+SEQIVIGTPGTVLDWCSKLKFIDPKKIKVFVLDEADVMIAT Sbjct: 190 ELKLAYAVRGNKLERGQKVSEQIVIGTPGTVLDWCSKLKFIDPKKIKVFVLDEADVMIAT 249 Query: 220 QGHQDQSIRIQRMLPRNCQMLLFSATFEDSVWKFAQKVVPDPNVIKLKREEETLDTIKQY 279 QGHQDQSIRIQR++PRNCQMLLFSATFEDSVWKFAQKVVPDPN+IKLKREEETLDTIKQY Sbjct: 250 QGHQDQSIRIQRIVPRNCQMLLFSATFEDSVWKFAQKVVPDPNIIKLKREEETLDTIKQY 309 Query: 280 YVLCSSRDEKFQALCNLYGAITIAQAMIFCHTRKTASWLAAELSKEGHQVALLΞGEMMVE 339 YVLC++R+EKFQALCNLYGAITIAQAMIFCHTRKTASWLAAELSKEGHQVALLSGEMMVE Sbjct: 310 YVLCNNREEKFQALCNLYGAITIAQAMIFCHTRKTASWLAAELSKEGHQVALLΞGEMMVE 369 Query: 340 QRAAVIERFREGKEKVLVTTNVCARGIDVEQVSVVINFDLPVDKDGNPDNETYLHRIGRT 399 QRAAVIERFREGKEKVLVTTNVCARGIDVEQVSVVINFDLPVDKDGNPDNETYLHRIGRT Sbjct: 370 QRAAVIERFREGKEKVLVTTNVCARGIDVEQVSVVINFDLPVDKDGNPDNETYLHRIGRT 429 Query: 400 GRFGKRGLAVNMVDSKHSMNILNRIQEHFNKKIERLDTDDLDEIEKIAN 448 GRFGKRGLAVNMVDSKHSMNILNRIQEHFNKKIERLDTDDLDEIEKIAN Sbjct: 430 GRFGKRGLAVNMVDSKHSMNILNRIQEHFNKKIERLDTDDLDEIEKIAN 478 Score : = 419 (62.9 bits), Expect = 3.8e-223, Sum P(2) = 3.8e-223 Identities : = 94/136 (69%), Positives = 104/136 (76%) Query: 1 MATDSWALAVDEQEAAAESLSNLHLKEEKIKPDTNGAVVKTNANAEKTDEEEKEDRAAQS 60 MATDSWALAVDEQEAA +S+S+L +KEEK K DTNG V+KT+ AEKT+EEEKEDRAAQS Sbjct: 1 MATDSWALAVDEQEAAVKSMSSLQIKEEKAKSDTNG-VIKTSTTAEKTEEEEKEDRAAQΞ 59 Query: 61 LLNKLIRSNLVDNTNQVEVLQRDPNSPLYSVKSFEELRL-PQNL IAQSQSGTGKTAA 116 LLNKLIRSNLVDNTNQVEVLQRDP+SPLYSVKSFEELRL PQ L A + K Sbjct: 60 LLNKLIRSNLVDNTNQVEVLQRDPSSPLYSVKSFEELRLKPQLLQGVYAMGFNRPSKIQE 119 Query: 117 FVLAMLSQVEPANKYPQ 133 L M+ P N Q Sbjct: 120 NALPMMLAEPPQNLIAQ 136 Pedant information for DKFZphfbr2_3cl8, frame 1 Report for DKFZphfbr2_3cl8.1 [LENGTH] 448 [MW] 50490.07 [pi] 5.83 [HOMOL] PIR:I49731 RNA helicase - mouse 0.0 [FUNCAT] 98 classification not yet clear-cut [S. cerevisiae, YOR046c] le-102 [FUNCAT] 04.01.04 rrna processing [S. cerevisiae, YDR021w] 2e-65 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YDR021w] 2e-65 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YJL138c] le-63 [FUNCAT] 05.04 translation (initiation, elongation and termination) [S. cerevisiae, YJL138C] le-63 [FUNCAT] 04.99 other transcription activities [S. cerevisiae, YDL160c] 2e-49 [FUNCAT] j mrna translation and ribosome biogenesis [H. influenzae, HI0231 RNA] 9e-4£ [FUNCAT] 04.05.03 mrna processing (splicing) [S. cerevisiae, YDL084w] le-43 [FUNCAT] 1 genome replication, transcription recombination and repair [H . in luenzae HI0892] 3e-39 [FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YLL008w] le-35 [FUNCAT] 09.01 biogenesis of cell wall [S. cerevisiae, YJL033w] 9e-27 [FUNCAT] 04.05.01.07 chromatin modification [S. cerevisiae, YMR290c] 8e-26 [FUNCAT] 30.16 mitochondrial organization [S. cerevisiae, YDR194C] le-23 [FUNCAT] r general function prediction [M. jannaschn, MJ1401] 9e-08 [FUNCAT] 11.10 cell death [S. cerevisiae, YMR190C] le-05 [FUNCAT] 03.19 recombination and dna repair [S. cerevisiae, YMR190c] le-05 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YIR002c] 7e-04 [BLOCKS] BL00039D DEAD-box subfamily ATP-dependent helicases proteins [BLOCKS] BL00039C DEAD-box subfamily ATP-dependent helicases proteins [BLOCKS] BL00039B DEAD-box subfamily ATP-dependent helicases proteins [BLOCKS] BL00039A DEAD-box subfamily ATP-dependent helicases proteins [PIRKW] nucleus 4e-64 [PIRKW] RNA binding le-64 [PIRKW] DEAD box 4e-64 [PIRKW] transmembrane protein 3e-22 [PIRKW] DNA binding 2e-32 [PIRKW] ATP le-101 [PIRKW] purine nucleotide binding 4e-64 [PIRKW] P-loop le-101 [PIRKW] hydrolase 4e-43 [PIRKW] protein biosynthesis le-64 [PIRKW] ATP binding 2e-35 [SUPFAM] WW repeat homology 3e-29 [SUPFAM] translation initiation factor eIF-4A le-64 [SUPFAM] DEAD/H box helicase homology le-101 [SUPFAM] DNA helicase recG 2e-06 [SUPFAM] unassigned DEAD/H box helicases le-101 [SUPFAM] ATP-dependent RNA helicase DBP1 9e-33 [SUPFAM] ATP-dependent RNA helicase DHH1 4e-48 [SUPFAM] tobacco ATP-dependent RNA helicase DB10 3e-29 [PROSITE] MYRISTYL 5 [PROSITE] AMIDATION 1 [PROSITE] CK2_PHOSPHO_SITE 6 [PROSITE] GLYCOSAMINOGLYCAN 1 [PROSITE] PKC_PHOSPHO_SITE 8 [PROSITE] ASN_GLYCOSYLATION 1 [PFAM] Helicases conserved C-terminal domain [PFAM] DEAD and DEAH box helicases [KW] Alpha_Beta SEQ MATDSWALAVDEQEAAAESLSNLHLKEEKIKPDTNGAVVKTNANAEKTDEEEKEDRAAQS PRD ccchhhhhhhhhhhhhhhhcccchhhhhhhcccccceeeeeehhhhhhhhhhhhhhhhhh SEQ LLNKLIRSNLVDNTNQVEVLQRDPNSPLYSVKSFEELRLPQNLIAQSQSGTGKTAAFVLA PRD hhhhhhhhhcccccceeeeeeccccccceeehhhhhhhhccceeeeeccccccchhhhhh SEQ MLSQVEPANKYPQCLCLSPTYELALQTGKVIEQMGKFYPELKLAYAVRGNKLERGQKISE PRD hhhhhhhhhccceeeeeccchhhhhhhhhhhhhhccccccccceeeccccchhhhhhhhe SEQ QIVIGTPGTVLDWCSKLKFIDPKKIKVFVLDEADVMIATQGHQDQSIRIQRMLPRNCQML PRD eeeecccccchhhhhhhhhhcccceeeeeecchhhhhhhccchhhhhhhhhhccccceee SEQ LFSATFEDSVWKFAQKVVPDPNVIKLKREEETLDTIKQYYVLCSSRDEKFQALCNLYGAI PRD eeeccccchhhhhhhhhhcccceeeehhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhch SEQ TIAQAMIFCHTRKTASWLAAELSKEGHQVALLSGEMMVEQRAAVIERFREGKEKVLVTTN PRD hhhhhheeecchhhhhhhhhhhhhccceeeeecccchhhhhhhhhhhhccccceeeeeec SEQ VCARGIDVEQVSVVINFDLPVDKDGNPDNETYLHRIGRTGRFGKRGLAVNMVDSKHSMNI PRD ccccccceeeeeeeeecccccccccccccceeeeeecccccccccceeeeeeeccchhhh SEQ LNRIQEHFNKKIERLDTDDLDEIEKIAN PRD hhhhhhhhhhhccccccccchhhhhccc Prosite for DKFZphfbr2_3cl8.1 PS00001 389->393 ASN_GLYCOSYLATION PDOC00001 PS00002 109->113 GLYCOSAMINOGLYCAN PDOC00002 PS00005 90->93 PKC_PHOSPHO_SITE PDOC00005 PS00005 111->114 PKC_PHOSPHO_SITE PDOC00005 PS00005 147->150 PKC_PHOSPHO_SITE PDOC00005 PS00005 226->229 PKC_PHOSPHO_SITE PDOC00005 PS00005 275->278 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 284->287 PKC_PHOSPHO_SITE PDOC00005 PS00005 311->314 PKC_PHOSPHO_SITE PDOC00005 PS00005 399->402 PKC_PHOSPHO_SITE PDOC00005 PS00006 48->52 CK2_PHOSPHO_SITE PDOC00006 PS00006 93->97 CK2_PHOSPHO_SITE PDOC00006 PS00006 123->127 CK2_PHOSPHO_SITE PDOC00006 PS00006 189->193 CK2_PHOSPHO_SITE PDOC00006 PS00006 245->249 CK2_PHOSPHO_SITE PDOC00006 PS00006 284->288 CK2_PHOSPHO_SITE PDOC00006 PS00008 110->116 MYRISTYL PDOC00008 PS00008 175->181 MYRISTYL PDOC00008 PS00008 185->191 MYRISTYL PDOC00008 PS00008 385->391 MYRISTYL PDOC00008 PS00008 406->412 MYRISTYL PDOC00008 PS00009 402->406 AMIDATION PDOC00009 Pfam for DKFZphfbr2_3cl8.1 HMM_NAME DEAD and DEAH box helicases HMM *gLpPWILRnIyeMGFEkPTPIQQqAIPιILeG.... RDVMACAQTGSGK ++ ++ +N ++ P E+ +++A++Q+G+GK Query 65 LIRSNLVDNTNQVEVLQRDPNSPLYΞVKSFEELRLPQNLIAQSQSGTGK 113 HMM TAAFlIPMLQHIDwdPWpqpPQdPrALILAPTRELAMQIQEEcRkFgkHM TAAF++ ML+++ + + PQ +L L+PT ELA+Q+ ++++++GK++ Query 114 TAAFVLAMLSQVEPAN—KYPQ CLCLSPTYELALQTGKVIEQMGKFY 158 HMM nglRImcIYGGtnMRdQMRmLeRGpPHIVIATPGRLIDHIER.gtldLDr + ++ + ++ ++ +++ +++ +IVI+TPG ++D + +D ++ Query 159 PELKLAYAVR GNKLERGQKISEQIVIGTPGTVLDWCSKLKFIDPKK 204 HMM IeMLVMDEADRMLD.MGFIDQIRrlMrqlPMpwNRQTMMFSATMPdelqE I+++V+DEAD M+ +G +DQ Rl R++P +N Q ++FSAT+ D++ + Query 205 IKVFVLDEADVMIATQGHQDQSIRIQRMLP—RNCQMLLFSATFEDSVWK 252 HMM LARrFMRNPIRInldMdElTtnEnlkQwYiyVerEMWKfdcLcrLIe* +A ++ +P I ++++E T++ +IKQ+Y+ + + ++KF +LC+L++ Query 253 FAQKVVPDPNVIKLKREEETLD-TIKQYYVLCSSRDEKFQALCNLYG 298 HMM_NAME Helicases conserved C-terminal domain HMM *EιleeWLknlGIrvmYIHGdMpQeERdeIMddFNnGEynVLIcTDVggR +L+ +L+++G +V+ + G M+ E+R ++++F++G+ +VL++T+V +R Query 316 SWLAAELSKEGHQVALLSGEMMVEQRAAVIERFREGKEKVLVTTNVCAR 364 HMM GIDIPdVNHVINYDM....PWNPEq.. YIQRIGRTgRIG* GID+++V++VIN+D+ + NP++ Y++RIGRTGR+G Query 365 GIDVEQVSVVINFDLPVDKDGNPDNETYLHRIGRTGRFG 403 Medline PMID: 10322435 "Unwinding RNA in DEAD-box proteins and related families." de la Cruz J, Kressler D, Linder P DKFZphfbr2 3fl6 group: brain derived DKFZphfbr2_3f16 encodes a novel 127 amino acid protein without similarity to known proteins. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of brain-specific genes . unknown complete cDNA, complete eds, EST hits Sequenced by AGOWA Locus : unknown Insert length: 1514 bp Poly A stretch at pos. 1454, polyadenylation signal at pos. 1434 1 GGGGGGACTG GAGAAGGGAG GCGGCGGGCG AAGCGCACGT CGAGCGGGGG 51 AGCGGCGCTG CCTGTGGAGA TCCGCGGAGG CCGACAGGAT TCGTTGGCTG 101 CCGTCCCCGC TGCTGTGCAT TGGGTTAAAA ACGACAACCA ACATCAGCCA 151 TGAAAGATCC AAGTCGCAGC AGTACTAGCC CAAGCATCAT CAATGAAGAT 201 GTGATTATTA ACGGTCATTC TCATGAAGAT GACAATCCAT TTGCAGAGTA 251 CATGTGGATG GAAAATGAAG AAGAATTCAA CAGACAAATA GAAGAGGAGT 301 TATGGGAAGA AGAATTTATT GAACGCTGTT TCCAAGAAAT GCTGGAAGAG 351 GAAGAAGAGC ATGAATGGTT TATTCCAGCT CGAGATCTCC CACAAACTAT 401 GGACCAAATC CAAGACCAGT TTAATGACCT TGTTATCAGT GAAGGCTCTT 451 CTCTGGAAGA TCTTGTGGTC AAGAGCAATC TGAATCCAAA TGCAAAGGAG 501 TTTGTTCCTG GGGTGAAGTA CGGAAATATT TGAGTAGACG GGGCCCTCTT 551 TTGGTGGATG TAGCACAATT TCCACACTGT GAAGGCAGTA TTAGAAGACT 601 TAATTGTAAA AGCACTCTTG TCACTGTGTT ACACTTATGC ATTGCCAAAG 651 TTTTTGTTAG TCTTGCATGC TTAATAAAAG TGCTGAGACT GTTACTAAGT 701 AAAAAGCTGT CAAACATTTA CTGAAAATAG AATTGGCCCC ATGGCTTGAT 751 GTGAAGACAG CAAGGAAAGA AGCACCAGTC AAGTTGTGAA CAAGCACCAA 801 ATTAAAAGAC CTAAACCTTA CCAAATTGTC TTTTTTTGAG GCTAATCTAT 851 CACTTGTTAA TGTCTAAACT TTAAAATCAG TACATTTAAT TTGAGTTCCA 901 ACTGTTAAGC ATATTTCTCA GACTTAAATT TGATTATGTC CCCATCAAAA 951 AGAATCTCCA TTTTCTGAAG GTCTGTTAGT TAATTTGAGA TAATTTGTTA 1001 AAGGCAAGTA TGTCATATTA CTGAGGCTAC AAGTTAGTCA GCAGATGAGT 1051 GCCAGTCCAG CCTTTTCCGG TATGTTATTG TTAGAAATAT TGAGTTCTAA 1101 TGTTACATCT GAGGAAGTAT GTAATTTGAG AATTGTAACT TCTAAGGGAT 1151 TCACTGCATC ATAGCTATGC CTGTATGGAG TCTAACATAT GACCAATACC 1201 AACCCATAAT CCAGCTGAAC AAAGATACTG TAACATTATG ATTTGAGTGG 1251 TGCTTTTCCT TGCTTTGTTA ACCATCACGA GAGTCTGCAG CACAACTTTT 1301 AACAAAGCTA GAACAGTTTT GGCTTCTTAA ACTTCATATT TGGGTAGGTT 1351 AAGCTGCCAT ACGTGTTCAG TGTGAATAGT GTTTAAGTTG AAAATATTGT 1401 AAAAAAATTA TATTTTTTCA AAAATATTTA AAAAAATAAA TAATAGTAGA 1451 ACTGAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAGAAAAA 1501 AAAAAAAAAA AAAA BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 3 ORF from 150 bp to 530 bp; peptide length: 127 Category: putative protein 1 MKDPSRSSTS PSIINEDVII NGHSHEDDNP FAEYMWMENE EEFNRQIEEE 51 LWEEEFIERC FQEMLEEEEE HEWFIPARDL PQTMDQIQDQ FNDLVISEGS 101 SLEDLVVKSN LNPNAKEFVP GVKYGNI BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_3f16, frame 3 No Alert BLASTP hits found Pedant information for DKFZphfbr2_3f16, frame 3 Report for DKFZphfbr2_3f16.3 [LENGTH] 127 [MW] 14998.41 [pi] 4.04 [BLOCKS] BL01269D [PROSITE] MYRISTYL 1 [PROSITE] CK2_PHOSPHO_SITE [KW] Alpha_Beta [KW] LOW COMPLEXITY 27.56 % SEQ MKDPSRSSTSPSIINEDVIINGHSHEDDNPFAEYMWMENEEEFNRQIEEELWEEEFIERC SEG xxxxxxxxxxxxxxxxxxxxxxx PRD ccccccccccccccccceeeecccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhh SEQ FQEMLEEEEEHEWFIPARDLPQTMDQIQDQFNDLVISEGSSLEDLVVKSNLNPNAKEFVP SEG xxxxxxxxxxxx PRD' hhhhhhhhhhhhhccccccccchhhhhhhhhcceeeecccccceeeeecccccccccccc SEQ GVKYGNI SEG PRD ccccccc Prosite for DKFZphfbr2_3f16.3 PS00006 24->28 CK2_PHOSPHO_SITE PDOC00006 PS00006 100->104 CK2_PHOSPHO_SITE PDOC00006 PS00008 121->127 MYRISTYL PDOC00008 (No Pfam data available for DKFZphfbr2 3fl6.3) DKFZphfbr2_3g8 group: metabolism DKFZphfbr2_3g8.1 encodes a novel 178 amino acid protein with similarity to yeast ARDl protein. In yeast, ARDl and NAT1, are required for the expression of an N-termmal protein acetyltransferase 1. NAT1 controls full repression of the silent mating type locus HML, sporulation and entry into GO. ARDl is involved in the assembly of the NAT 1-complex. The new protein could be part of this or an other NAT complex. The new protein can find application modulating NAT assembly and action and therefore be important in metabolism of drugs and environmental mutagens. strong similarity to N-TERMINAL ACETYLTRANSFERASE COMPLEX ARDl homolog complete cDNA, complete eds? start at Bp 40, EST hits Sequenced by AGOWA Locus: /map="20" Insert length: 1030 bp Poly A stretch at pos. 1013, no polyadenylation signal found 1 TGGGCTTGGC GAACGGTCTT CGGAAGCGGC GGCGGCGCGA TGACCACGCT 51 ACGGGCCTTT ACCTGCGACG ACCTGTTCCG CTTCAACAAC ATTAACTTGG 101 ATCCACTTAC AGAAACTTAT GGGATTCCTT TCTACCTACA ATACCTCGCC 151 CACTGGCCAG AGTATTTCAT TGTTGCAGTG GCACCTGGTG GAGAATTAAT 201 GGGTTATATT ATGGGTAAAG CAGAAGGCTC AGTAGCTAGG GAAGAATGGC 251 ACGGGCACGT CACAGCTCTG TCTGTTGCCC CAGAATTTCG ACGCCTTGGT 301 TTGGCTGCTA AACTTATGGA GTTACTAGAG GAGATTTCAG AAAGAAAGGG 351 TGGGTTTTTT GTGGATCTCT TTGTAAGAGT ATCTAACCAA GTTGCAGTTA 401 ACATGTACAA GCAGTTGGGC TACAGTGTAT ATAGGACGGT CATAGAGTAC 451 TATTCGGCCA GCAACGGGGA GCCTGATGAG GACGCTTATG ATATGAGGAA 501 AGCACTTTCC AGGGATACTG AGAAGAAATC CATCATACCA TTACCTCATC 551 CTGTGAGGCC TGAAGACATT GAATAACCCT GGGCAGTGGT TCTTAGGCAG 601 ATACTCTAGA TGCTTTATGG ACAATATTAT TTTCATTGGA TGATTCTGGA 651 GCTCTATTAG GAGAAAAGTA ATCATTTTAG GTCTTAAAGA CTTCAAGAAA 701 ATACAGGTTA TCAATTTATT TTAAATCTCA TTGTTTCCAG TTAGCAATAT 751 CATACCTATT AAAGCTGTTC ATTGTAACAA AATTCAATCA AAAAGGCAGC 801 TAGGTCAGAA GGAAACATAC CACTCTCATG GTTCATAGTA TTCACTGTAT 851 GTATGCTAGG GAAAAGACTT GCTCCAGTCT CCTCCTCAGT TCTGTGCCTG 901 AGAACCACTG CTGCATATAT TTGTTTTTAA ATTTTGTATT GAACTGTTAA 951 TTGAAGCTTT AAAAGCATAT ATGAAATGTA TAAATCTAAG ATGTATAATA 1001 CATTATTGAC TCCAAAAAAA AAAAAAAAAA BLAST Results Entry HSG0101 from database EMBL: human STS SHGC-35956. Length = 401 Minus Strand HSPs: Score = 1417 (212.6 bits), Expect = 9.3e-58, P = 9.3e-5S Identities = 301/311 (96%) Medline entries No Medline entry Peptide information for frame 1 ORF from 40 bp to 573 bp; peptide length: 178 Category: strong similarity to known protein 1 MTTLRAFTCD DLFRFNNINL DPLTETYGIP FYLQYLAHWP EYFIVAVAPG 51 GELMGYIMGK AEGSVAREEW HGHVTALSVA PEFRRLGLAA KLMELLEEIS 101 ERKGGFFVDL FVRVSNQVAV NMYKQLGYSV YRTVIEYYSA SNGEPDEDAY 151 DMRKALSRDT EKKSIIPLPH PVRPEDIE BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_3g8, frame 1 TREMBL :SPCC16C4_12 gene: "SPCC16C4.12"; product: "putative n-terminal acetyltransferase complex subunit"; S. pombe chromosome III cosmid C16C4., N = 1, Score = 475, P = 3.2e-45 SWISSPROT:ARDH_LEIDO N-TERMINAL ACETYLTRANSFERASE COMPLEX ARDl SUBUNIT HOMOLOG., N = 1, Score = 451, P = 1. le-42 PIR:S69021 hypothetical protein YPR131C - yeast (Saccharomyces cerevisiae), N = 1, Score = 382, P = 2.3e-35 >TREMBL:SPCC16C4_12 gene: "SPCC16C4.12"; product: "putative n-terminal acetyltransferase complex subunit"; S. pombe chromosome III cosmid cl6C4. Length = 180 HSPs: Score = 475 (71.3 bits), Expect = 3.2e-45, P = 3.2e-45 Identities = 96/165 (58%), Positives = 118/165 (71%) Query: 1 MTTLRAFTCDDLFRFNNINLDPLTETYGIPFYLQYLAHWPEYFIVAVAPGGE—LMGYIM 58 MT R F DLF FNNINLDPLTET+ I FYL YL WP +V + + LMGYIM Sbjct: 1 MTDTRKFKATDLFSFNNINLDPLTETFNISFYLSYLNKWPSLCVVQESDLSDPTLMGYIM 60 Query: 59 GKAEGSVAREEWHGHVTALSVAPEFRRLGLAAKLMELLEEISERKGGFFVDLFVRVSNQV 118 GK+EG+ +EWH HVTA++VAP RRLGLA +M+ LE + + FFVDLFVR SN + Sbjct: 61 GKSEGT—GKEWHTHVTAITVAPNSRRLGLARTMMDYLETVGNSENAFFVDLFVRASNAL 118 Query: 119 AVNMYKQLGYSVYRTVIEYYSASNGEPDEDAYDMRKALSRDTEKKSI 165 A++ YK LGYSVYR VI YYS +G+ DED++DMRK LSRD ++SI Sbjct: 119 AIDFYKGLGYSVYRRVIGYYSNPHGK-DEDSFDMRKPLSRDVNRESI 164 Pedant information for DKFZphfbr2_3g8, frame 1 Report for DKFZphfbr2_3g8.1 [LENGTH] 178 [MW] 20338.24 [pi] 5.06 [HOMOL] TREMBL:SPCC16C4_12 gene: "SPCC16C4.12"; product: "putative n-terminal acetyltransferase complex subunit"; S. pombe chromosome III cosmid cl6C4. 7e-47 [FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylation, farnesylation and processing) [S. cerevisiae, YPR131c] 6e-37 [ FUNCAT ] 01.06.07 lipid, fatty-acid and sterol utilization [S. cerevisiae, YHR013c] 4e-14 [FUNCAT] 30.03 organization of cytoplasm [S cerevisiae, YHR013c] 4e-14 [FUNCAT] 03.22 cell cycle control and mitosis [S cerevisiae, YHR013c] 4e-14 [FUNCAT] r general function prediction [M jannaschn, MJ1530] 6e-09 [PIRKW] acyltransferase le-12 [SUPFAM] arrest-defective protein 1 le-12 [SUPFAM] Escherichia coli peptide N-acetyltransferase riml le-07 [PROSITE] CK2_PHOSPHO_SITE 3 [PROSITE] PKC_PHOSPHO_SITE 3 [KW] Alpha_Beta SEQ MTTLRAFTCDDLFRFNNINLDPLTETYGIPFYLQYLAHWPEYFIVAVAPGGELMGYIMGK PRD ccccccccccchhhhhhcccccccccccchhhhhhcccccceeeeeeccccceeeehhhh SEQ AEGSVAREEWHGHVTALSVAPEFRRLGLAAKLMELLEEISERKGGFFVDLFVRVSNQVAV PRD hcccccccccccceeeeehhhhhhhhcchhhhhhhhhhhhhhccceeeeeeeecchhhhh SEQ NMYKQLGYSVYRTVIEYYSASNGEPDEDAYDMRKALSRDTEKKSIIPLPHPVRPEDIE PRD hhhhhhcccchhhhhhccccccccccchhhhhhhhhhhhhhhhhcccccccccccccc Prosite for DKFZphfbr2_3g8.1 PS00005 3->6 PKC_PHOSPHO_SITE PDOC00005 PS00005 100->103 PKC_PHOSPHO_SITE PDOC00005 PS00005 160->163 PKC_PHOSPHO_SITE PDOC00005 PS00006 8->12 CK2_PHOSPHO_SITE PDOC00006 PS00006 133->137 CK2_PHOSPHO_SITE PDOC00006 PS00006 141->145 CK2 PHOSPHO SITE PDOC00006 (No Pfam data available for DKFZphfbr2_3g8.1) DKFZphfbr2_312 group: brain derived DKFZphfbr2_312 encodes a novel 589 amino acid protein with weak similarity to S. cerevisiae ubiquitin-like protein DSK2. Pfam predicts for this protein similarity to the ubiquitin family; No informative BLAST results; No predictive prosite or SCOP motive The new protein can find application in studying the expression profile of brain-specific genes . similarity to ubiquitm-like protein DSK2 yeast complete cDNA, complete eds, EST hits Dsk2p is involved m spindel pole body SPB duplication, SPB = centomer strong similarity to HRIHFB2157 human mRNA Sequenced by AGOWA Locus: unknown Insert length: 2978 bp Poly A stretch at pos. 2958, polyadenylation signal at pos. 2924 1 GGGGGGAGGA AGCGGTGGCT GCTGCGGATG TCGGTGTGAG CGAGCGGCGC 51 CTGAACACAC GGCGGCTGCC GAGCGCCTGA CCCGGGCCTG CGCCAGAGCC 101 TGCACCGAGC TCCGGGGCCC CACACCCGCT ACGGTGGCCC TGCGCCCGTT 151 GCTACTGAGG CGGCGTGCTC TGCATTCTTC GCTGTCCAGG CCTGCCGGCT 201 CTGGTGTCTG CTGGCTCCTC CTTGCTCGCC TGCTCCCTCC TGCTTGCCTG 251 AGTCACCGCC GCCGCCGCCG CCACAGCCAT GGCCGAGAGT GGTGAAAGCG 301 GCGGTCCTCC GGGCTCCCAG GATAGCGCCG CCGGAGCCGA AGGTGCTGGC 351 GCCCCCGCGG CCGCTGCCTC CGCGGAGCCC AAAATCATGA AAGTCACCGT 401 GAAGACCCCG AAGGAAAAGG AGGAATTCGC CGTGCCCGAG AATAGCTCCG 451 TCCAGCAGTT TAAGGAAGAA ATCTCTAAAC GTTTTAAATC ACATACTGAC 501 CAACTTGTGT TGATATTTGC TGGAAAAATT TTGAAAGATC AAGATACCTT 551 GAGTCAGCAT GGAATTCATG ATGGACTTAC TGTTCACCTT GTCATTAAAA 601 CACAAAACAG GCCTCAGGAT CATTCAGCTC AGCAAACAAA TACAGCTGGA 651 GGCAATGTTA CTACATCATC AACTCCTAAT AGTAACTCTA CATCTGGTTC 701 TGCTACTAGC AACCCTTTTG GTTTAGGTGG CCTTGGGGGA CTTGCAGGTC 751 TGAGTAGCTT GGGTTTGAAT ACTACCAACT TCTCTGAACT ACAGAGTCAG 801 ATGCAGCGAC AACTTTTGTC TAACCCTGAA ATGATGGTCC AGATCATGGA 851 AAATCCCTTT GTTCAGAGCA TGCTCTCAAA TCCTGACCTG ATGAGACAGT 901 TAATTATGGC CAATCCACAA ATGCAGCAGT TGATACAGAG AAATCCAGAA 951 ATTAGTCATA TGTTGAATAA TCCAGATATA ATGAGACAAA CGTTGGAACT 1001 TGCCAGGAAT CCAGCAATGA TGCAGGAGAT GATGAGGAAC CAGGACCGAG 1051 CTTTGAGCAA CCTAGAAAGC ATCCCAGGGG GATATAATGC TTTAAGGCGC 1101 ATGTACACAG ATATTCAGGA ACCAATGCTG AGTGCTGCAC AAGAGCAGTT 1151 TGGTGGTAAT CCATTTGCTT CCTTGGTGAG CAATACATCC TCTGGTGAAG 1201 GTAGTCAACC TTCCCGTACA GAAAATAGAG ATCCACTACC CAATCCATGG 1251 GCTCCACAGA CTTCCCAGAG TTCATCAGCT TCCAGCGGCA CTGCCAGCAC 1301 TGTGGGTGGC ACTACTGGTA GTACTGCCAG TGGCACTTCT GGGCAGAGTA 1351 CTACTGCGCC AAATTTGGTG CCTGGAGTAG GAGCTAGTAT GTTCAACACA 1401 CCAGGAATGC AGAGCTTGTT GCAACAAATA ACTGAAAACC CACAACTGAT 1451 GCAAAACATG TTGTCTGCCC CCTACATGAG AAGCATGATG CAGTCACTAA 1501 GCCAGAATCC TGACCTTGCT GCACAGATGA TGCTGAATAA TCCCCTATTT 1551 GCTGGAAATC CTCAGCTTCA AGAACAAATG AGACAACAGC TCCCAACTTT 1601 CCTCCAACAA ATGCAGAATC CTGATACACT ATCAGCAATG TCAAACCCTA 1651 GAGCAATGCA GGCCTTGTTA CAGATTCAGC AGGGTTTACA GACATTAGCA 1701 ACGGAAGCCC CGGGCCTCAT CCCAGGGTTT ACTCCTGGCT TGGGGGCATT 1751 AGGAAGCACT GGAGGCTCTT CGGGAACTAA TGGATCTAAC GCCACACCTA 1801 GTGAAAACAC AAGTCCCACA GCAGGAACCA CTGAACCTGG ACATCAGCAG 1851 TTTATTCAGC AGATGCTGCA GGCTCTTGCT GGAGTAAATC CTCAGCTACA 1901 GAATCCAGAA GTCAGATTTC AGCAACAACT GGAACAACTC AGTGCAATGG 1951 GATTTTTGAA CCGTGAAGCA AACTTGCAAG CTCTAATAGC AACAGGAGGT 2001 GATATCAATG CAGCTATTGA AAGGTTACTG GGCTCCCAGC CATCATAGCA 2051 GCATTTCTGT ATCTTGAAAA AATGTAATTT ATTTTTGATA ACGGCTCTTA 2101 AACTTTAAAA TACCTGCTTT ATTTCATTTT GACTCTTGGA ATTCTGTGCT 2151 GTTATAAACA AACCCAATAT GATGCATTTT AAGGTGGAGT ACAGTAAGAT 2201 GTGTGGGTTT TTCTGTATTT TTCTTTTCTG GAACAGTGGG AATTAAGGCT 2251 ACTGCATGCA TCACTTCTGC ATTTATTGTA ATTTTTTAAA AACATCACCT 2301 TTTATAGTTG GGTGACCAGA TTTTGTCCTG CATCTGTCCA GTTTATTTGC 2351 TTTTTAAACA TTAGCCTATG GTAGTAATTT ATGTAGAATA AAAGCATTAA 2401 AAAGAAGCAA ATCATTTGCA CTCTATAATT TGTGGTACAG TATTGCTTAT 2451 TGTGACTTTG GCATGCATTT TTGCAAACAA TGCTGTAAGA TTTATACTAC 2501 TGATAATTTT GTTTTATTTG TATACAATAT AGAGTATGCA CATTTGGGAC 2551 TGCATTTCTG GAAACATACT GCAATAGGCT CTCTGAGCAA AACACCTGTA 2601 ACTAAAAAAG TGAAGATAAG AAAATACTCT TAAAGCTGAG TATTTCCTAA 2651 TTGTATAGAA TCTTACAGCA TCTTTGACAA ACATCTCCCA GCAAAAGTGC 2701 CGGTTAGTCA GGTTTGTTGA AAATACAGTA GAAAAGCTGA TTCTGGTTAT 2751 CTCTTTAAGG ACAATTAATT GTACAGACAC ATAATGTAAC ATTGTCTCAA 2801 CATTCATTCA CAGATTGACT GTAAATTACC TTAATCTTTG TGCAGACTGA 2851 AGGAACACTG TAGTATACCC CAAAGTGCAT TTGCCTAGGA CTTCTCAGCT 2901 TCTCCCATAG GTAGTTTAAC AGGCATTAAA ATTTGTAATT GAAATGTTGC 2951 TTTCACTCAA AAAAAAAAAA AAAAAAAA BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 3 ORF from 279 bp to 2045 bp; peptide length: 589 Category: similarity to known protein 1 MAESGESGGP PGSQDSAAGA EGAGAPAAAA SAEPKIMKVT VKTPKEKEEF 51 AVPENSSVQQ FKEEISKRFK SHTDQLVLIF AGKILKDQDT LSQHGIHDGL 101 TVHLVIKTQN RPQDHSAQQT NTAGGNVTTS STPNSNSTSG SATSNPFGLG 151 GLGGLAGLSS LGLNTTNFSE LQSQMQRQLL SNPEMMVQIM ENPFVQSMLS 201 NPDLMRQLIM ANPQMQQLIQ RNPEISHMLN NPDIMRQTLE LARNPAMMQE 251 MMRNQDRALS NLESIPGGYN ALRRMYTDIQ EPMLSAAQEQ FGGNPFASLV 301 SNTSSGEGΞQ PSRTENRDPL PNPWAPQTSQ SSSASSGTAS TVGGTTGSTA 351 SGTSGQSTTA PNLVPGVGAS MFNTPGMQSL LQQITENPQL MQNMLSAPYM 401 RSMMQSLSQN PDLAAQMMLN NPLFAGNPQL QEQMRQQLPT FLQQMQNPDT 451 LSAMSNPRAM QALLQIQQGL QTLATEAPGL IPGFTPGLGA LGSTGGSSGT 501 NGSNATPSEN TSPTAGTTEP GHQQFIQQML QALAGVNPQL QNPEVRFQQQ 551 LEQLSAMGFL NREANLQALI ATGGDINAAI ERLLGSQPS BLASTP hits Entry CE1_1 from database TREMBL: "F15C11.2"; Caenorhabditis elegans cosmid VF15C11L Length = 293 Score = 454 (159.8 bits), Expect = 4.4e-43, P = 4.4e-43 Identities = 81/162 (50%), Positives = 113/162 (69%) Entry S54583 from database PIR: ubiquitin-like protein DSK2 - yeast (Saccharomyces cerevisiae) Length = 373 Score = 278 (97.9 bits), Expect = 1.2e-23, P = 1.2e-23 Identities = 100/307 (32%), Positives = 155/307 (50%) Entry AB015344_1 from database TREMBLNEW: gene: "HRIHFB2157"; Homo sapiens HRIHFB2157 mRNA, partial eds. Score = 1135, P = 3.6e-115, identities = 227/301, positives = 253/301 Alert BLASTP hits for DKFZphfbr2_312, frame 3 No Alert BLASTP hits found Pedant information for DKFZphfbr2_312, frame 3 Report for DKFZphfbr2 312.3 [LENGTH] 589 [MW] 62489.22 [pi] 5.02 [HOMOL] TREMBL :AB015344_1 gene: "HRIHFB2157"; Homo sapiens HRIHFB2157 mRNA, partial cds. le-121 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YMR276w] 2e-17 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YMR276w] 2e-17 [BLOCKS] BL00299 Ubiquitin family proteins [SUPFAM] unassigned ubiquitin-related proteins 5e-16 [SUPFAM] ubiquitin homology 5e-16 [PROSITE] MYRISTYL 24 [PROSITE] CK2_PHOSPHO_SITE [PROSITE] GLYCOSAMINOGLYCAN [PROSITE] PKC_PHOSPHO_SITE [PROSITE] ASN_GLYCOSYLATION [PFAM] Ubiquitin family [KW] Irregular [KW] 3D [KW] LOW COMPLEXITY 23.43 % SEQ MAESGESGGPPGSQDSAAGAEGAGAPAAAAΞAEPKIMKVTVKTPKEKEEFAVPENSΞVQQ SEG ..xxxxxxxxxxx..xxxxxxxxxxxxxxxxxxx...xxxxxxxxxxxx laarA CEEEEEETTTCEEEECTTTTBHHH SEQ FKEEISKRFKΞHTDQLVLIFAGKILKDQDTLSQHGIHDGLTVHLVIKTQNRPQDHSAQQT SEG laarA HHHHHHHHHCCCGGGEEEEETTEECTTTTBGGGGCCTTTTEEEEEBC SEQ NTAGGNVTTSSTPNSNSTSGSATSNPFGLGGLGGLAGLSSLGLNTTNFSELQSQMQRQLL SEG ... xxxxxxxxxxxxxxxxxxxxxx ..xxxxxxxxxxxxxxxx laarA SEQ SNPEMMVQIMENPFVQSMLSNPDLMRQLIMANPQMQQLIQRNPEISHMLNNPDIMRQTLE SEG laarA SEQ LARNPAMMQEMMRNQDRALSNLESIPGGYNALRRMYTDIQEPMLSAAQEQFGGNPFASLV SEG laarA SEQ SNTSSGEGSQPSRTENRDPLPNPWAPQTSQSSSASSGTASTVGGTTGSTASGTSGQSTTA SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx laarA SEQ PNLVPGVGASMFNTPGMQSLLQQITENPQLMQNMLSAPYMRSMMQSLSQNPDLAAQMMLN SEG laarA SEQ NPLFAGNPQLQEQMRQQLPTFLQQMQNPDTLSAMSNPRAMQALLQIQQGLQTLATEAPGL SEG laarA SEQ IPGFTPGLGALGSTGGSSGTNGSNATPSENTSPTAGTTEPGHQQFIQQMLQALAGVNPQL SEG .... xxxxxxxxxxxxxxxxxxxxxxxx laarA SEQ QNPEVRFQQQLEQLSAMGFLNREANLQALIATGGDINAAIERLLGSQPS SEG laarA Prosite for DKFZphfbr2_312.3 PS00001 55->59 ASN_GLYCOSYLATION PDOC00001 PS00001 126->130 ASN_GLYCOSYLATION PDOC00001 PS00001 136->140 ASN_GLYCOSYLATION PDOC00001 PS00001 164->168 ASN_GLYCOSYLATION PDOC00001 PS00001 167->171 ASN_GLYCOSYLATION PDOC00001 PS00001 302->306 ASN_GLYCOSYLATION PDOC00001 PS00001 501->505 ASN_GLYCOSYLATION PDOC00001 PS00002 305->309 GLYCOSAMINOGLYCAN PDOC00002 PS00005 40->43 PKC_PHOSPHO_SITE PDOC00005 PS00005 43->46 PKC_PHOSPHO_SITE PDOC00005 PS00005 66->69 PKC_PHOSPHO_SITE PDOC00005 PS00006 43->47 CK2_PHOSPHO_SITE PDOC00006 PS00006 71->75 CK2_PHOSPHO_SITE PDOC00006 PS00006 181->185 CK2_PHOSPHO_SITE PDOC00006 PS00006 200->204 CK2_PHOSPHO_ΞITE PDOC00006 PS00006 260->264 CK2_PHOSPHO_SITE PDOC00006 PS00006 304->308 CK2_PHOSPHO_SITE PDOC00006 PS00006 312->316 CK2_PHOSPHO_SITE PDOC00006 PΞ00006 506->510 CK2_PHOΞPHO_SITE PDOC00006 PS00006 572->576 CK2_PHOSPHO_SITE PDOC00006 PS00008 8->14 MYRISTYL PDOC00008 PS00008 12->18 MYRISTYL PDOC00008 SO o o ca H U α. r o + : + M > > J ^ X + > + O Q X + + X + a σ 3 σ DKFZphfbr2_62bll group: signal transduction DKFZphfbr2_62bll .encodes a novel 655 amino ac d putative GTPase-activating protein, related to human chimaerins . The rac small GTPase is associated with type-I phosphatidylinositol 4-phosphate 5-kιnase and regulating the production of phosphatidylinositol 4, 5-bιsphosphate . The new protein is expected to activate p21rac-related small GTPases. The new protein can find clinical application in modulating/blocking the response to a cellular receptor. similarity to CHIMAERIN complete cDNA, complete eds, EST hits Sequenced by LMU Locus: /map="4" Insert length: 4593 bp Poly A stretch at pos. 4571, polyadenylation signal at pos. 4553 1 GGGGGAGTTT GAAGACAGAA AGGAAAGGGG AGAAACCTGC AGAGAGCATC 51 AAAGGATGGG GGGTGCTATA AAAGAAGCAG GGGGGTCCTT TGAAAGAAAT 101 CTATCATGCA CTGAAATGCT TTCTGGAGAA GGTGCCGTTA TTTTCCTCCC 151 CTCTTGCTCA GATGAAAGGA GCCAGCAAGG ACAGTCCTGA AATATTCCTC 201 AGGGGACTTT TTGTCATTGT TCCTCTTTCC TCTTGCACAG AGCTATTTGC 251 TGACCTTTCC AGAGGAATCT CAGTCCAGCT GAGAAGACAG TTCTTAATAA 301 AAACAAAAAA ATGCAAAAAC CAATTCCTGC TGTTTGAATG GGAATGGTAG 351 CTTGCTTGCT GCAGTTCTTT TCCTGTGACA TTTTGGAATG TCTGCAGAAA 401 CTTAAAAAAA AGAAAAAAAA AACCTTAAAA ACTCCCTGGA TTAGGCAAGA 451 GAAAAGGAAG TTTTTTTTTG CTAAACAGGA GTAAATGAGA GGTGGTAACT 501 TATCCCTAAG CCAGGACCTG GATGATCAAA ACCTTCAAAT TCTAGGGATC 551 AGCACTTCAA AAATAACAAG TAAACAAGCA TGAGGAGTGG CTGTTGGGTT 601 TCGCTCAGAG GCAGGTTTTA AAGGAAGCCA AAACCGGGTT CAGAACTTCA 651 GGCCTGTACG ATGCCTGAAG ACCGGAATTC TGGGGGGTGC CCGGCTGGTG 701 CCTTAGCCTC AACTCCTTTC ATCCCTAAAA CTACATACAG AAGAATCAAA 751 CGGTGTTTTA GTTTTCGGAA AGGCATTTTT GGACAGAAAC TGGAGGATAC 801 TGTTCGTTAT GAGAAGAGAT ATGGGAACCG TCTGGCTCCG ATGTTGGTGG 851 AGCAGTGCGT GGACTTTATC CGACAAAGGG GGCTGAAAGA AGAGGGTCTC 901 TTTCGACTGC CAGGCCAGGC TAATCTTGTT AAGGAGCTCC AAGATGCCTT 951 TGACTGTGGG GAGAAGCCAT CATTTGACAG CAACACAGAT GTACACACGG 1001 TGGCATCACT TCTTAAGCTG TACCTCCGAG AACTTCCAGA ACCAGTTATT 1051 CCTTATGCGA AGTATGAAGA TTTTTTGTCA TGTGCCAAAC TGCTCAGCAA 1101 GGAAGAGGAA GCAGGTGTTA AGGAATTAGC AAAGCAGGTG AAGAGTTTGC 1151 CAGTGGTAAA TTACAACCTC CTCAAGTATA TTTGCAGATT CTTGGATGAA 1201 GTACAGTCCT ACTCGGGAGT TAACAAAATG AGTGTGCAGA ACTTGGCAAC 1251 GGTCTTTGGT CCTAATATCC TGCGCCCCAA AGTGGAAGAT CCTTTGACTA 1301 TCATGGAGGG CACTGTGGTG GTCCAGCAGT TGATGTCAGT GATGATTAGC 1351 AAACATGATT GCCTCTTTCC CAAAGATGCA GAACTACAAA GCAAGCCCCA 1401 AGATGGAGTG AGCAACAACA ATGAAATTCA GAAGAAAGCC ACCATGGGGC 1451 TGTTACAGAA CAAGGAGAAC AATAACACCA AGGACAGCCC TAGTAGGCAG 1501 TGCTCCTGGG ACAAGTCTGA GTCACCCCAG AGAAGCAGCA TGAACAATGG 1551 ATCCCCCACA GCTCTATCAG GCAGCAAAAC CAACAGCCCA AAGAACAGTG 1601 TTCACAAGCT AGATGTGTCT AGAAGCCCCC CTCTCATGGT CAAAAAGAAC 1651 CCAGCCTTTA ATAAGGGTAG TGGGATAGTT ACCAATGGGT CCTTCAGCAG 1701 CAGTAATGCA GAAGGTCTTG AGAAAACCCA AACCACCCCC AATGGGAGCC 1751 TACAGGCCAG AAGGAGCTCT TCACTGAAGG TATCTGGTAC CAAAATGGGC 1801 ACGCACAGTG TACAGAATGG AACGGTGCGC ATGGGCATTT TGAACAGCGA 1851 CACACTCGGG AACCCCACAA ATGTTCGAAA CATGAGCTGG CTGCCAAATG 1901 GCTATGTGAC CCTGAGGGAT AACAAGCAGA AAGAACAAGC TGGAGAGTTA 1951 GGCCAGCACA ACAGACTGTC CACCTATGAT AATGTCCATC AACAGTTCTC 2001 CATGATGAAC CTTGATGACA AGCAGAGCAT TGACAGTGCT ACCTGGTCCA 2051 CTTCCTCCTG TGAAATCTCC CTCCCTGAGA ACTCCAACTC CTGTCGCTCT 2101 TCTACCACCA CCTGCCCAGA GCAAGACTTT TTTGGGGGGA ACTTTGAGGA 2151 CCCTGTTTTG GATGGGCCCC CGCAGGACGA CCTTTCCCAC CCCAGGGACT 2201 ATGAAAGCAA AAGTGACCAC AGGAGTGTGG GAGGTCGAAG TAGTCGTGCC 2251 ACCAGTAGCA GTGACAACAG TGAGACATTT GTGGGCAACA GCAGCAGCAA 2301 CCACAGTGCA CTGCACAGTT TAGTTTCCAG CCTGAAACAG GAAATGACCA 2351 AACAGAAGAT AGAGTATGAG TCCAGGATAA AGAGCTTAGA ACAGCGAAAC 2401 TTGACTTTGG AAACAGAAAT GATGAGCCTC CATGATGAAC TGGATCAGGA 2451 GAGGAAAAAG TTCACAATGA TAGAAATAAA AATGCGAAAT GCCGAGCGAG 2501 CAAAAGAAGA TGCCGAGAAA AGAAATGACA TGCTACAGAA AGAAATGGAG 2551 CAGTTTTTTT CCACGTTTGG AGAACTGACA GTGGAACCCA GGAGAACCGA 2601 GAGAGGAAAC ACAATATGGA TTCAGTGAGC CTGCTTTCGC CTGCTGTCTC 2651 TGATGGCTCT GGCAAGGACT CCAGGGATTC TGGTGGGATA TGACTTAGAA 2701 CCAGGTGGCT GGTCACCTGG ATGTACAGAA GTCTAACTGG TGAAGGAATA 2751 TCATTTACAG ACATTAAACA TCCATATCTG CAATGTGTAC CAAAGTTATA 2801 TCATGCCCCA TAATGCTACT GTCAAGTGTT ACAACTGGAT ATGTGTATAT 2851 AGAGTAGTTT TTCAAAAGTA AACTAAAAAT GAGAAGCATA TTTCAAGAAT 2901 TATTTTATTG CAAGTCTTGT ATTTAAATGT TAAATCAATA TGTTGTTGCA 2951 ATTTAGCTTG CTTTCAAGCT TCACCCCTTG CACTTAACAT AAGCTATTTT 3001 TGGCATTGTG TTATCATCGG CTTATTTTAT AGATCAATAT TTTTATTTCC 3051 CTTTTTTGCT GAGGAAATGA AGATAAGCAA AAATATAAAT ATATATATAA 3101 ATATATGAGT TATTAAAACC AGAAGAATAC TTTGTGGCTG TGCTGTTTGT 3151 GCCAATAGAC TTTGTCATGA CCAAAAAGAG AAATGTAAAT AGTTTTATAA 3201 AATACAGTCG AATCACCAGG AACCTTTGAG CTGCTTTTAA AATTCTTCCC 3 251 CTGGCACCAC TCAGTTTTGC TTTTGCGAGG CGATTTGACA TAGGAACTTT 3301 GAGACTCCAT GAGAAAGTCC CTTTCTGAGG CCCACTGTCT ACCTTGCCAG 3351 ATCCTCAGTG CGTATCGCCA ATGCAGGATG CTCCTTAGAA AAGAAAAAAT 3401 GGTAAAGGAT GGCATTTAAC GATTCAGGCT TTGAATTACT CTGTCCCTCT 3451 GGACCGAATC TCTTTAACTG CTGGATAGTT TTAGAGGAAT TCTCCTGCTA 3501 CTTAGGTACT GGGAAACAAT GCTTGCTAAA CCATGCCCAC GTGAGCACCT 3551 GTCTCCCACT CAAACCTCTC CCATCTCCCA ACAACTGCAC TTTAGAATAC 3601 CAGCAGTGAA ATGGTATTAC TGTTTCCCTC TGAGTGAAAC TGCTAGAGTA 3651 TATGTCACGT AGTGACATTT TTTTCTCACT CAGGCTATTG CCATCTGGGA 3701 TTCTCTCCCT ACTACAGCTG GCAAAGTTGG TTTGCAGCAA GAAGATAGTG 3751 GGAGGGGGCC AGGCTGCAGG AGAAGGAGAA AAGTTTAGAA GAAACAAACC 3801 ATTTTGCTTC TAATTTTGAC AGTATCACTT TCCTGTTAAA ACATACAATA 3851 ATTTTAAAAG GTGAATGCCT AAAGTTCCAA TTTTAGCAAA TATGGGAACC 3901 TCAGCAATGC TAATTTTCTA GAAAAACCCA GGGCTCTTTG GAGCTAGAGT 3951 TTTGGGAGAA CAGTTCTTCA CAATAAGGCA ATGGTTTTGA GAGGCCAGGC 4001 AAATAATCTT TCTCACCGTA GAACAAAAAG TTACAAAAGG CATAATCGGA 4051 AATAGAGACT ACATACTTGA GTTTATGGGG TTTGTGTTGT TTGAAGGTTC 4101 AATGCTTGCA TGTGTTTATT TATTTTCAAG AGGGAAAGTG GTCTGTACTG 4151 CTTTCATCCT TGCCACTGTC TTGCTTTTAT TTTTTACTCT CCCACTGAGC 4201 AAGCGTCTGT GGTCCTATGG TATCAACCAG TATCTTTATA GCAATAATTT 4251 CTTTAATTCC CTTTTCTCTC TCTTTCCAAT TATTTAACCA GTTACTTCCA 4301 CCTGGACATA CGATAGGAAA TTCAAACTCA AAATATGAAA ATTGATCTTA 4351 ATAACTCTCC CTTCATATCT TTTCACCTAT TTCCAGTCCT TATCATAGTT 4401 GATAAAAACC TCAGACTCAT CCAGAAAGCT ATATGATGCA CTAGTAAAAA 4451 AAACAAAGAT ATTTAAACTG CTTGGGTTCA AATGGTATAC AATTTGCCAG 4501 CTGTTACTGA ACCTTCTATG CATAACTTTT TTTTTCCTCT GTGCAATTGG 4551 AATAATAAAA ATACTACTCC CATAAAAAAA AAAAAAAAAA AAC BLAST Results Entry G38474 from database EMBLNEW: SHGC-58303 Human Homo sapiens STS genomic, sequence tagged site. Score = 2175, P = 1.2e-92, identities = 439/441 Medline entries 97476250: Beta2-ch maeπn is a high affinity receptor for the phorbol ester tumor promoters . Peptide information for frame 1 ORF from 661 bp to 2625 bp; peptide length: 655 Category: similarity to known protein 1 MPEDRNSGGC PAGALASTPF IPKTTYRRIK RCFSFRKGIF GQKLEDTVRY 51 EKRYGNRLAP MLVEQCVDFI RQRGLKEEGL FRLPGQANLV KELQDAFDCG 101 EKPSFDSNTD VHTVASLLKL YLRELPEPVI PYAKYEDFLS CAKLLSKEEE 151 AGVKELAKQV KSLPVVNYNL LKYICRFLDE VQSYSGVNKM SVQNLATVFG 201 PNILRPKVED PLTIMEGTVV VQQLMSVMIS KHDCLFPKDA ELQSKPQDGV 251 SNNNEIQKKA TMGLLQNKEN NNTKDSPSRQ CSWDKSESPQ RSSMNNGSPT 301 ALSGSKTNSP KNSVHKLDVS RSPPLMVKKN PAFNKGSGIV TNGSFSSSNA 351 EGLEKTQTTP NGSLQARRSS SLKVSGTKMG THSVQNGTVR MGILNSDTLG 401 NPTNVRNMSW LPNGYVTLRD NKQKEQAGEL GQHNRLSTYD NVHQQFSMMN 451 LDDKQSIDSA TWSTSSCEIS LPENSNSCRS STTTCPEQDF FGGNFEDPVL 501 DGPPQDDLSH PRDYESKSDH RSVGGRSSRA TSSSDNSETF VGNSSSNHSA 551 LHSLVSSLKQ EMTKQKIEYE ΞRIKSLEQRN LTLETEMMSL HDELDQERKK 601 FTMIEIKMRN AERAKEDAEK RNDMLQKEME QFFSTFGELT VEPRRTERGN 651 TIWIQ BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_62bll, frame 1 SWISSPROT:Y053_HUMAN HYPOTHETICAL PROTEIN KIAA0053., N = 3, Score = 661, P = 2.4e-89 TREMBL :HSU90908_1 product: "unknown"; Human clones 23549 and 23762 mRNA, complete eds., N = 1, Score = 348, P = l.le-29 PIR:S29128 N-chimerin - rat, N = 1, Score = 286, P = 2.8e-24 PIR:S29956 beta-chimerin - rat, N = 1, Score = 279, P = 1.6e-23 TREMBL:AB014572_1 gene: "KIAA0672"; product: "KIAA0672 protein"; Homo sapiens mRNA for KIAA0672 protein, complete eds., N = 1, Score = 314, P = le-24 >SWISSPROT:Y053_HUMAN HYPOTHETICAL PROTEIN KIAA0053. Length = 638 HSPs: Score = 661 (99.2 bits), Expect = 2.4e-89, Sum P(3) = 2.4e-89 Identities = 122/209 (58%), Positives = 160/209 (76%) Query: 38 GIFGQKLEDTVRYEKRYGNRLAPMLVEQCVDFIRQRGLKEEGLFRLPGQANLVKELQDAF 97 G+FGQ+L++TV YE+++G L P+LVE+C +FI + G EEG+FRLPGQ NLVK+L+DAF Sbjct: 148 GVFGQRLDETVAYEQKFGPHLVPILVEKCAEFILEHGRNEEGIFRLPGQDNLVKQLRDAF 207 Query: 98 DCGEKPSFDSNTDVHTVASLLKLYLRELPEPVIPYAKYEDFLSCAKLLSKEEEAGVKELA 157 D GE+PSFD +TDVHTVAΞLLKLYLR+LPEPV+P+++YE FL C +L + +E +EL Sbjct: 208 DAGERPSFDRDTDVHTVASLLKLYLRDLPEPVVPWSQYEGFLLCGQLTNADEAKAQQELM 267 Query: 158 KQVKSLPVVNYNLLKYICRFLDEVQSYSGVNKMSVQNLATVFGPNILRPKVEDPLTIMEG 217 KQ+ LP NY+LL YICRFL E+Q VNKMSV NLATV G N++R KVEDP IM G Sbjct: 268 KQLSILPRDNYΞLLSYICRFLHEIQLNCAVNKMSVDNLATVIGVNLIRSKVEDPAVIMRG 327 Query: 218 TVVVQQLMSVMISKHDCLFPKDAELQSKP 246 T +Q++M++MI H+ LFPK ++ P Sbjct: 328 TPQIQRVMTMMIRDHEVLFPKSKDIPLSP 356 Score = 210 (31.5 bits), Expect = 2.4e-89, Sum P(3) = 2.4e-89 Identities = 45/115 (39%), Positives = 73/115 (63%) Query: 531 TSSSDNSETFVGNSSSNHSALHSL VΞSLKQEMTKQKIEYESRIKSLEQRNLTLETEM 587 T +S NSET G +Ξ + SL V L++E+ QK YE +IK+LE+ N + ++ Sbjct: 523 TLASPNSETGPGKKNSGEEEIDSLQRMVQELRKEIETQKQMYEEQIKNLEKENYDVWAKV 582 Query: 588 MSLHDELDQERKKFTMIEIKMRNAERAKEDAEKRNDMLQKEMEQFFSTFGELTVE 642 + L++EL++E+KK +EI +RN ER++ED EKRN L++E+++F + E E Sbjct: 583 VRLNEELEKEKKKSAALEIΞLRNMERSREDVEKRNKALEEEVKEFVKSMKEPKTE 637 Score = 70 (10.5 bits), Expect = 1.2e-74, Sum P(3) = 1.2e-74 Identities = 28/121 (23%), Positives = 54/121 (44%) Query: 528 ΞRATSSSDNSETFVGNSΞSNHSALHSLVSSLKQE-MTKQKIEYESRIKSLEQRNL-TLET 585 S+ TS+ DN + G+ SAL S K + + E K+ + + +L+ Sbjct: 489 SQRTSTYDNVPSLPGSPGEEASALSSQACDSKGDTLASPNSETGPGKKNSGEEEIDSLQR 548 Query: 586 EMMSLHDELDQERKKFTMIEIKMRNAERAKEDAEKRNDMLQKEMEQFFSTFGELTVEPRR 645 + L E++ +++ M E +++N E+ D + L +E+E+ L + R Sbjct: 549 MVQELRKEIETQKQ MYEEQIKNLEKENYDVWAKVVRLNEELEKEKKKSAALEISLRN 605 Query: 646 TER 648 ER Sbjct: 606 MER 608 Score = 53 (8.0 bits), Expect = 2.4e-89, Sum P(3) = 2.4e-89 Identities = 31/111 (27%), Positives = 46/111 (41%) Query: 344 SFSSSNAEGLEKTQTTPNGSLQARRSSSLKVSGTKMGTHSVQNG TV--RMGILNSD 397 SFSS ++ + T T A S KV K G +Q+ T+ R L S Sbjct: 388 SFSSMTSDS-DTTSPTGQQPSDAFPEDSSKVPREKPGDWKMQSRKRTQTLPNRKCFLTSA 446 Query: 398 TLG-NPTNV RNMSWLPNGYVTLRDNKQKEQAGELGQ HNRLSTYDNV 442 G N + + +N W P+ + ++ + +L Q R STYDNV Sbjct: 447 FQGANSSKMEIFKNEFWSPSSEAKAGEGHRRTMSQDLRQLSDSQRTSTYDNV 498 Score = 53 (8.0 bits), Expect = 3.5e-14, Sum P(3) = 3.5e-14 Identities = 32/125 (25%), Positives = 56/125 (44%) Query: 242 LQSKPQDG VSNNNEIQKKATMGLLQNKEN--NNTKD SPSRQCSWDKSESPQRΞS 293 ++SK +D + +IQ+ TM ++++ E +KD SP Q + K RSS Sbjct: 314 IRSKVEDPAVIMRGTPQIQRVMTM-MIRDHEVLFPKSKDIPLSPPAQKNDPKKAPVARSS 372 Query: 2 94 MNNGSPTALSGSKTNSPKNSVHKLDVSRSPPLMVKKNPAFNKGSGIVTNGSFSSSNAEGL 353 + + L S+T+S + D + P + + AF + S V + Sbjct: 373 VGWDATEDLRISRTDSFSSMTSDSDTTS—PTGQQPSDAFPEDSSKVPREKPGDWKMQSR 430 Query: 354 EKTQTTPN 361 ++TQT PN Sbjct: 431 KRTQTLPN 438 Pedant information for DKFZphfbr2_62bll, frame 1 Report for DKFZphfbr2_62bll .1 [LENGTH] 655 [MW] 73394.60 [pi] 8.13 [HOMOL] SWISSPROT:Y053_HUMAN HYPOTHETICAL PROTEIN KIAA0053. 3e-71 [FUNCAT] 03.07 pheromone response, mating-type determination, sex-specific proteins [S. cerevisiae, YPL115c] le-16 [FUNCAT] 09.04 biogenesis of cytoskeleton [S. cerevisiae, YPL115C] le-16 [FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YPL115c] le-16 [FUNCAT] 10.02.09 regulation of g-protein activity [S. cerevisiae, YPL115c] le-16 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YER155c] 2e-16 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YER155C] 2e-16 [FUNCAT] 10.99 other signal-transduction activities [S. cerevisiae, YDR379w] 4e-16 [FUNCAT] 03.10 sporulation and germination [S. cerevisiae, YDL240w] 3e-15 [FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YOR134w] 2e-13 [FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YOR134w] 2e-13 [SCOP] dlrgp 1.83.1.1.1 p50 RhoGAP domain [human (Homo sapiens) 2e-46 [SCOP] dlpbwa_ 1.83.1.1.2 p85 alpha subunit RhoGAP domain [human (Hom 6e-37 [PIRKW] phosphotransferase 3e-13 [PIRKW] breakpoint cluster region 2e-20 [PIRKW] transmembrane protein 7e-14 [PIRKW] brain 2e-20 [PIRKW] alternative splicing 2e-20 [PIRKW] P-loop 9e-19 [PIRKW] cytoskeleton le-08 [SUPFAM] CDC24 homology 7e-21 [SUPFAM] bcr protein 7e-21 [SUPFAM] myosin motor domain homology 9e-19 [SUPFAM] pleckstπn repeat homology 2e-15 [SUPFAM] LIM metal-bindmg repeat homology 9e-15 [SUPFAM] protein kinase C zinc-binding repeat homology 5e-24 [PROSITE] MYRISTYL 16 [PROSITE] CAMP_PHOSPHO_SITE 3 [PROSITE] CK2_PHOSPHO_SITE 15 [PROSITE] TYR_PHOSPHO_SITE 2 [PROSITE] PKC_PHOSPHO_SITE 11 [PROSITE] ASN_GLYCOSYLATION 8 [KW] Irregular [KW] 3D [KW] LOW_COMPLEXITY 6.87 % [KW] COILED COIL 12.06 % SEQ MPEDRNSGGCPAGALASTPFIPKTTYRRIKRCFSFRKGIFGQKLEDTVRYEKRYGNRLAP SEG COILS 1rgp- C SEQ MLVEQCVDFIRQRGLKEEGLFRLPGQANLVKELQDAFDCGEKPSFDSNTDVHTVASLLKL SEG COILS lrgp- HHHHHHHHHHHHHHTTTTTTTTTCCCHHHHHHHHHHHHHCCCCCGGGCCCCHHHHHHHHH SEQ YLRELPEPVIPYAKYEDFLSCAKLLSKEEEAGVKELAKQVKΞLPVVNYNLLKYICRFLDE SEG COILS lrgp- HHHHTTTTTTTGGGHHHHHH TTTTCGGGHHHHHHHHHHHCCHHHHHHHHHHHHHHHH SEQ VQSYSGVNKMSVQNLATVFGPNILRPKVEDPLTIMEGTVVVQQLMSVMISKHDCLFPKDA SEG COILS lrgp- HHHHHHHHCCCHHHHHHHHGGGCC SEQ ELQSKPQDGVSNNNEIQKKATMGLLQNKENNNTKDSPSRQCSWDKSESPQRSSMNNGSPT SEG COILS lrgp- SEQ ALSGSKTNSPKNSVHKLDVSRΞPPLMVKKNPAFNKGSGIVTNGSFSSSNAEGLEKTQTTP SEG COILS lrgp- SEQ NGSLQARRSSSLKVSGTKMGTHSVQNGTVRMGILNSDTLGNPTNVRNMSWLPNGYVTLRD SEG COILS lrgp- SEQ NKQKEQAGELGQHNRLSTYDNVHQQFSMMNLDDKQΞIDSATWSTSSCEISLPENSNSCRS SEG xxxxxx COILS lrgp- SEQ STTTCPEQDFFGGNFEDPVLDGPPQDDLSHPRDYESKSDHRSVGGRSSRATSSSDNSETF SEG xxxxx xxxxxxxxxxxxxxxx ... COILS lrgp- SEQ VGNSSSNHSALHSLVSSLKQEMTKQKIEYESRIKSLEQRNLTLETEMMSLHDELDQERKK SEG .. xxxxxxxxxxxxxxxx COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC lrgp- SEQ FTMIEIKMRNAERAKEDAEKRNDMLQKEMEQFFSTFGELTVEPRRTERGNTIWIQ SEG COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC lrgp- Prosite for DKFZphfbr2_62bll .1 PS00001 271->275 ASN_GLYCOSYLATION PDOC00001 PS00001 342->346 ASN_GLYCOSYLATION PDOC00001 PS00001 361->365 ASN_GLYCOSYLATION PDOC00001 PS00001 386->390 ASN_GLYCOSYLATION PDOC00001 PS00001 407->411 ASN_GLYCOSYLATION PDOC00001 PS00001 543->547 ASN_GLYCOSYLATION PDOC00001 PS00001 547->551 AΞN_GLYCOSYLATION PDOC00001 PS00001 580->584 ASN_GLYCOS LATION PDOC00001 PS00004 258->262 CAMP_PHOSPHO_SITE PDOC00004 PS00004 367->371 CAMP_PHOSPHO_SITE PDOC00004 PS00004 599->603 CAMP_PHOSPHO_SITE PDOC00004 PS00005 25->28 PKC_PHOSPHO_SITE PDOC00005 PS00005 34->37 PKC_PHOSPHO_SITE PDOC00005 PS00005 47->50 PKC_PHOSPHO_SITE PDOC00005 PS00005 309->312 PKC_PHOSPHO_SITE PDOC00005 PS00005 371->374 PKC_PHOSPHO_SITE PDOC00005 PS00005 388->391 PKC_PHOSPHO_SITE PDOC00005 PS00005 417->420 PKC_PHOSPHO_SITE PDOC00005 PS00005 477->480 PKC_PHOSPHO_SITE PDOC00005 PS00005 527->530 PKC_PHOSPHO_SITE PDOC00005 PS00005 557->560 PKC_PHOSPHO_SITE PDOC00005 PS00005 646->649 PKC_PHOSPHO_SITE PDOC00005 PS00006 107-M11 CK2_PHOSPHO_SITE PDOC00006 PS00006 146->150 CK2_PHOSPHO_SITE PDOC00006 PS00006 213->217 CK2_PHOSPHO_SITE PDOC00006 PΞ00006 230->234 CK2_PHOSPHO_SITE PDOC00006 PS00006 348->352 CK2_PHOSPHO_SITE PDOC00006 PS00006 417->421 CK2_PHOSPHO_SITE PDOC00006 PS00006 437->441 CK2_PHOSPHO_SITE PDOC00006 PS00006 465->469 CK2_PHOSPHO_SITE PDOC00006 PS00006 470->474 CK2_PHOSPHO_SITE PDOC00006 PS00006 484->488 CK2_PHOSPHO_SITE PDOC00006 PS00006 516->520 CK2_PHOSPHO_SITE PDOC00006 PS00006 532->536 CK2 PHOSPHO SITE PDOC00006 SO o o ca H U α. o ooooooooooooooooooooo O ooooooooooooooooooooo ooooooooooooooooooooo ooooooooooooooooooooo o DKFZphfbr2_62flO group: intracellular transport and trafficking DKFZphfbr2_62f10 encodes a novel 320 amino acid protein with strong similarity to mammalian zinc transporter proteins. The novel proteins is a membrane protein, which should be involved in the transport of Zmc across the cell membrane. The Zn-T-transporters are membrane proteins that facilitates sequestration of zinc m endosomal vesicles. In the brain, ZnT-3 mRNA seems to be involved in the accumulation of zmc in synaptie vesicles. Zinc (Zn) is an essential element in normal development and metabolism. Recent studies show that in Alzheimer s disease, Zn functions as a double-edged sword, affording protection against Alzheimer s amyloid beta peptide (the major component of senile plaques) at low concentrations and enhancing toxicity at high concentrations by accelerated aggregation of the amyloid beta peptide. The new protein can find application in modulation of Zinc transport in neuronal cells, thus providing means for a modulation of Alzheimer's amyloid beta peptide plaque formation. strong similarity to zinc transporter proteins ; membrane regions : 5 Summary DKFZphfbr2_62f10 encodes a novel 320 amino acid protein with similarity to zinc transporter protein. The new protein can find clinical application m modulating Zn2+ uptake . strong similarity to zmc transporter proteins complete cDNA, complete eds, few EST hits Sequenced by LMU Locus : unknown Insert length: 5422 bp Poly A stretch at pos. 5397, polyadenylation signal at pos. 5381 1 GTCTAACTTT GGAAATATCA CCCTCATGCT GTCTTCCCAG GATGTCTCTC 51 TCCCTAAGTA AGGGATGTTA CTTCCTGGAG GGAATGCAGT GTTGGGAATC 101 TGAAGACCCA GCTTTGAGCT GAATTTGCTT TGTGATACCT GGAGAGAAGA 151 CGTGTTTTCT TGACAACAGC ACAGTACCTA GTGAGTTCAA CAACAACGAC 201 AACAACAGCC GCAGCTCATC CTGGCCGTCA TGGAGTTTCT TGAAAGAGCG 251 TATCTTGTGA ATGATAAAGC TGCCAAGATG TATGCTTTCA CACTAGAAAG 301 AAGGAGCTGC AAATGAACAC TTCATAGCAA TGTGGAACTC CAACAGAAAC 351 CGGTGAATAA AGATCAGTGT CCCAGAGAGA GACCAGAGGA GCTGGAGTCA 401 GGAGGCATGT ACCACTGCCA CAGTGGCTCC AAGCCCACAG AAAAGGGGGC 451 GAATGAGTAC GCCTATGCCA AGTGGAAACT CTGTTCTGCT TCAGCAATAT 501 GCTTCATTTT CATGATTGCA GAGGTCGTGG GTGGGCACAT TGCTGGGAGT 551 CTTGCTGTTG TCACAGATGC TGCCCACCTC TTAATTGACC TGACCAGTTT 601 CCTGCTCAGT CTCTTCTCCC TGTGGTTGTC ATCGAAGCCT CCCTCTAAGC 651 GGCTGACATT TGGATGGCAC CGAGCAGAGA TCCTTGGTGC CCTGCTCTCC 701 ATCCTGTGCA TCTGGGTGGT GACTGGCGTG CTAGTGTACC TGGCATGTGA 751 GCGCCTGCTG TATCCTGATT ACCAGATCCA GGCGACTGTG ATGATCATCG 801 TTTCCAGCTG CGCAGTGGCG GCCAACATTG TACTAACTGT GGTTTTGCAC 851 CAGAGATGCC TTGGCCACAA TCACAAGGAA GTACAAGCCA ATGCCAGCGT 901 CAGAGCTGCT TTTGTGCATG CCCCTGGAGA TCTATTTCAG AGTATCAGTG 951 TGCTAATTAG TGCACTTATT ATCTACTTTA AGCCAGAGTA TAAAATAGCC 1001 GACCCAATCT GCACATTCAT CTTTTCCATC CTGGTCTTGG CCAGCACCAT 1051 CACTATCTTA AAGGACTTCT CCATCTTACT CATGGAAGGT GTGCCAAAGA 1101 GCCTGAATTA CAGTGGTGTG AAAGAGCTTA TTTTAGCAGT CGACGGGGTG 1151 CTGTCTGTGC ACTGCCTGCA CATCTGGTCT CTAACAATGA ATCAAGTAAT 1201 TCTCTCAGCT CATGTTGCTA CAGCAGCCAG CCGGGACAGC CAAGTGGTTC 1251 GGAGAGAAAT TGCTAAAGCC CTTAGCAAAA GCTTTACGAT GCACTCACTC 1301 ACCATTCAGA TGGAATCTCC AGTTGACCAG GACCCCGACT GCCTTTTCTG 1351 TGAAGACCCC TGTGACTAGC TCAGTCACAC CGTCAGTTTC CCAAATTTGA 1401 CAGGCCACCT TCAAACATGC TGCTATGCAA TTTCTGCATC ATAGAAAATA 1451 AGGAACCAAA GGAAGAAATT CATGTCATGG TGCAATGCAT ATTTTATCTA 1501 TTTATTTAGT TCCATTCACC ATGAAGGAAG AGGCACTGAG ATCCATCAAT 1551 CAATTGGATT ATATACTGAT CAGTAGCTGT GTTCAATTGC AGGAATGTGT 1601 ATATAGATTA TTCCTGAGTG GAGCCGAAGT AACAGCTGTT TGTAACTATC 1651 GGCAATACCA AATTCATCTC CCTTCCAATA ATGCATCTTG AGAACACATA 1701 GGTAAATTTG AACTCAGGAA AGTCTTACTA GAAATCAGTG GAAGGGACAA 1751 ATAGTCACAA AATTTTACCA AAACATTAGA AACAAAAAAT AAGGAGAGCC 1801 AAGTCAGGAA TAAAAGTGAC TCTGTATGCT AACGCCACAT TAGAACTTGG 1851 TTCTCTCACC AAGCTGTAAT GTGATTTTTT TTTCTACTCT GAATTGGAAA 1901 TATGTATGAA TATACAGAGA AGTGCTTACA ACTAATTTTT ATTTACTTGT 1951 CACATTTTGG CAATAAATCC CTCTTATTTC TAAATTCTAA CTTGTTTATT 2001 TCAAAACTTT ATATAATCAC TGTTCAAAAG GAAATATTTT CACCTACCAG 2051 AGTGCTTAAA CACTGGCACC AGCCAAAGAA TGTGGTTGTA GAGACCCAGA 2101 AGTCTTCAAG AACAGCCGAC AAAAACATTC GAGTTGACCC CACCAAGTTG 2151 TTGCCACAGA TAATTTAGAT ATTTACCTGC AAGAAGGAAT AAAGCAGATG 2201 CAACCAATTC ATTCAGTCCA CGAGCATGAT GTGAGCACTG CTTTGTGCTA 2251 GACATTGGGC TTAGCACTGA AACTATAAAG AGGAATCAGA CGCAGCAAGT 2301 GCTTCTGTGT TCTGGTAGCA ACTCAACACT ATCTGTGGAG AGTAAACTGA 2351 AGATGTGCAG GCCAACATTC TGGAAATCCT ATGTCAGTGG GTTTGGTTTG 2401 GAACCTGGAC TTCTGCATTT TTAAAAGTTA CCCAGAGATG CTTCTAAAGA 2451 TGAGCCATAG TCTAGAAGAT TGTCAACCAC AGGAGTTCAT TGAGTGGGAC 2501 AGCTAGACAC ATACATTGGC AGTTACAATA GTATCATGAA TTGCAATGAT 2551 GTAGTGGGGT ATAAAAGGAA AGCGATGGAT ATTGCCGGAT GGGCATGGCC 2601 AGTGATGTTT CACGTCATTG AGGTGACAGC TCTGCTGGAC TTTGAATTAC 2651 ATATGGAGGC TCTCCAGGAA GACGAAGAAG AGAAGGACAT TCTAGGCAAA 2701 AAGAAGACTA GGCACAAGGC ACACTTATGT TTGTCTGTTA GCTTTTAGTT 2751 GAAAAAGCAA AATACATGAT GCAAAGAAAC CTCTCCACGC TGTGATTTTT 2801 AAAACTACAT ACTTTTTGCA ACTTTATGGT TATGAGTATT GTAGAGAACA 2851 GGAGATAGGT CTTAGATGAT TTTTATGTTG TTGTCAGACT CTAGCAAGGT 2901 ACTAGAAACC TAGCAGGCAT TAATAATTGT TGAGGCAATG ACTCTGAGGC 2951 TATATCTGGG CCTTGTCATT ATTTATCATT TATATTTGTA TTTTTTTCTG 3001 AAATTTGAGG GCCAAGAAAA CATTGACTTT GACTGAGGAG GTCACATCTG 3051 TGCCATCTCT GCAAATCAAT CAGCACCACT GAAATAACTA CTTAGCATTC 3101 TGCTGAGCTT TCCCTGCTCA GTAGAGACAA ATATACTCAT CCCCCACCTC 3151 AGTGAGCTTG TTTAGGCAAC CAGGATTAGA GCTGCTCAGG TTCCCAACGT 3201 CTCCTGCCAC ATCGGGTTCT CAAAATGGAA AGAATGGTTT ATGCCAAATC 3251 ACTTTTCCTG TCTGAAGGAC CACTGAATGG TTTTGTTTTT CCATATTTTG 3301 CATAGGACGC CCTAAAGACT AGGTGACTTG GCAAACACAC AAGTGTTAGT 3351 ATAATTCTTT GCTTCTGCTT CTTTTTGAAA ATCATGTTTA GATTTGATTT 3401 TAAGTCAGAA ATTCACTGAA TGTCAGGTAA TCATTATGGA GGGAGATTTG 3451 TGTGTCAACC AAAGTAATTG TCCCATGGCC CCAGGGTATT TCTGTTGTTT 3501 CCCTGAAATT CTGCTTTTTT AGTCAGCTAG ATTGAAAACT CTGAACAGTA 3551 GATGTTTATA TGGCAAAATG CAAGACAATC TATAAGGGAG ATTTTAAGGA 3601 TTTTGAGATG AAAAAACAGA TGCTACTCAG GGGCTTTATG GACCATCCAT 3651 CAATTCTGAA GTTCTGACTC TCCCATTACC CTTTCCCTGG TGTGGTCAGA 3701 ACTCCAGGTC ACTGGAAGTT AGTGGAATCA TGTAGTTGAA TTCTTTACTT 3751 CAAGACATTG TATTCTCTCC AGCTATCAAA ACATTAATGA TCTTTTATGT 3801 CTTTTTTTTG TTATTGTTAT ACTTTAAGTT CTGGGGTACA TGTGCGGAAC 3851 ATGTAGGTTT GTTACATAGG TATACATGTG CCATGGTGGT TTGCTGCACT 3901 CATCAACCTG TCATCTACAT TCTTTTATGT CTGTCTTTCA AAGCAACACT 3951 CTGTTCTTCT GAGTAGTGAA ATCAGGTCAA CTTTACCACC AGCCTCCATT 4001 TTTAATATGC TTCACCATCA TCCAGCACCT ACTTAAGATT TATCTAGGGC 4051 TCTGTGGTGA TGTTAGGACC CATAAAAGAA ATTTATGCCT TCCATATGTT 4101 TGGTTACAGA TGGGAAATGG GAATGTTGAA GGACATGAAA GAAAGGATGT 4151 TTACACATTA AGCATCAGTT CTGAAGCTAG ATTGTCTGAG TTTGAATCTT 4201 AGCTCTTCCC TTTATTAGCT CTGTGACCTC GAGCTAGTTA CTTAAATGCT 4251 CTGATCCTCT ATTTCCTGAT CAGTGAAACC TCCCTATTCA AATGTGTGAG 4301 AGTTTAATAA ATTAGGACAC TTAAAAATGT TGGAGCAGTG CATAGCATGT 4351 AGTGTTCAGT ACATGTTAAA TGTTGTTTTT TATTATGTAC AAACATGTGT 4401 GGGCACAGAA TTTTAAATCA TCTCAACTTT TGAGAAATTT TGAGTTATCA 4451 ACACCGTTCC CACAAGACAG TGGCAAAATT ATTGGTGAGA ATTAAACAGC 4501 TGTTTCTCAG AGGAAGCAAT GGAGGCTTGC TGGGATAAAG GCATTTACTG 4551 AGAGGCTGTT ACCTAGTGAG AGTGATGAAT TAATTAAAAT AGTCGAATCC 4601 CTTTCTGACT GTCTCTGAAA GCTTCCGCTT TTATCTTTGA AGAGCAGAAT 4651 TGTCACCCCA AGGACATTTA TTAATAAAAA GAACAACTGT CCAGTGCAAT 4701 GAAGGCAAAG TCATAGGTCT CCCAAGTCTT ACCCCATTCC TGTGAAATAT 4751 CAAGTTCTTG GCTTTTCTCT GTCATGTAGC CTCAACTTTC TCCGACCGGG 4801 TGCATTTCTT TCTCTGGTTT CTAAATTGCC AGTGGCAAAT TTGGATCACT 4851 TACTTAATAT CTGTTAAATT TTGTGACCCA ACAAAGTCTT TTAGCACTGT 4901 GGTGTCAAAA AGAAAAACAC CTCCCAGGCA TATACATTTT ATAGATTCCT 4951 GGAGAATGTT GCTCTCCAGC TCCATCCCCA CCCAATGAAA TATGATCCAG 5001 AGAGTCTTGC AAAGAGACAA GCCTCATTTT CCACAATTAG CTCTAAAGTG 5051 CCTCCAGGAA ATGATTTTCT CAGCTCATCT CTCTGTATTC CCTGTTTTGG 5101 ATCACAGGGC AATCTGTTTA AATGACTAAT TACAGAAATC ATTAAAGGCA 5151 CCAAGCAAAT GTCATCTCTG AATACACACA TCCCAAGCTT TACAAATCCT 5201 GCCTGGCTTG ACAGTGATGA GGCCACTTAA CAGTCCAGCG CAGGCGGATG 5251 TTAAAAAAAA TAAAAAGGTG ACCATCTGCG GTTTAGTTTT TTAACTTTCT 5301 GATTTCACAC TTAACGTCTG TCATTCTGTT ACTGGGCACC TGTTTAAATT 5351 CTATTTTAAA ATGTTAATGA GTGTTGTTTA AAATAAAATC AGGAAAGAGA 5401 GAAAAAAAAA AAAAAAAAAA AC BLAST Results o BLAST result Medline entries 97121493: ZnT-3, a putative transporter of zinc into synaptie vesicles. 96203098: ZnT-2, a mammalian protein that confers resistance to zmc by facilitating vesicular sequestration . Peptide information for frame 2 ORF from 407 bp to 1366 bp; peptide length: 320 Category: strong similarity to known protein 1 MYHCHSGSKP TEKGANEYAY AKWKLCSASA ICFIFMIAEV VGGHIAGSLA 51 VVTDAAHLLI DLTSFLLSLF SLWLSSKPPΞ KRLTFGWHRA EILGALLSIL 101 CIWVVTGVLV YLACERLLYP DYQIQATVMI IVSSCAVAAN IVLTVVLHQR 151 CLGHNHKEVQ ANASVRAAFV HAPGDLFQSI ΞVLISALIIY FKPEYKIADP 201 ICTFIFSILV LASTITILKD FSILLMEGVP KSLNYSGVKE LILAVDGVLS 251 VHCLHIWSLT MNQVILSAHV ATAASRDSQV VRREIAKALS KSFTMHSLTI 301 QMESPVDQDP DCLFCEDPCD BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_62f10, frame 2 PIR:S70632 zinc transporter ZnT-2 - rat, N = 1, Score = 884, P = 1.5e-88 TREMBL :MMU76007_1 gene: "ZnT-3"; product: "ZnT-3"; Mus musculus zmc transporter ZnT-3 (ZnT-3) mRNA, complete eds., N = 1, Score = 772, P = l.le-76 TREMBL:HSU76010_1 gene: "ZnT-3"; product: "ZnT-3"; Human putative zmc transporter ZnT-3 (ZnT-3) mRNA, complete eds., N = 1, Score = 74 2 , P = 1.6e-73 TREMBL :MMUZNT02_1 gene: "ZnT-3"; product: "zinc transporter"; Mus musculus zinc transporter (ZnT-3) gene, complete eds., N = 1, Score = 715, P = 1.2e-70 TREMBL :CET18D3_3 gene: "T18D3.3"; Caenorhabditis elegans cosmid T18D3, N = 1, Score = 699, P = 5.9e-69 >PIR:S70632 zinc transporter ZnT-2 - rat Length = 359 HSPs: Score = 884 (132.6 bits), Expect = 1.5e-88, P = 1.5e-88 Identities = 171/326 (52%), Positives = 230/326 (70%) Query: 2 YHCHSGSKPTEKGANEYAYAKWKLCSASAICFIFMIAEVVGGHIAGSLAVVTDAAHLLID 61 ++CH+ +E A+ KL ASAIC +FMI E++GG++A SLA++TDAAHLL D Sbjct: 34 HYCHAQKDSGSHPNΞEKQRARRKLYVASAICLVFMIGEIIGGYLAQΞLAIMTDAAHLLTD 93 Query: 62 LTSFLLSLFSLWLΞSKPPSKRLTFGWHRAEILGALLSILCIWVVTGVLVYLACERLLYPD 121 S L+SLFSLW+SS+P +K + FGW RAEILGALLS+L IWVVTGVLVYLA +RL+ D Sbjct: 94 FASMLISLFSLWVSSRPATKTMNFGWQRAEILGALLSVLSIWVVTGVLVYLAVQRLISGD 153 Query: 122 YQIQATVMIIVSSCAVAANIVLTVVLHQRCLGHNH KEVQANASVRAAFVHAPG 174 Y+I+ M+I S CAVA NI++ + LHQ GH+H + Q N SVRAAF+H G Sbjct: 154 YEIKGDTMLITSGCAVAVNIIMGLALHQSGHGHSHGHSHEDSΞQQQQNPSVRAAFIHVVG 213 Query: 175 DLFQSISVLISALIIYFKPEYKIADPICTFIFΞILVLASTITILKDFSILLMEGVPKSLN 234 DL QS+ VL++A IIYFKPEYK DPICTF+FSILVL +T+TIL+D ++LMEG PK ++ Sbjct: 214 DLLQSVGVLVAAYIIYFKPEYKYVDPICTFLFSILVLGTTLTILRDVILVLMEGTPKGVD 273 Query: 235 YSGVKELILAVDGVLSVHCLHIWSLTMNQVILSAHVATAASRDSQVVRREIAKALSKSFT 294 ++ VK L+L+VDGV ++H LHIW+LT+ Q +LS H+A A + D+Q V + L F Sbjct: 274 FTTVKNLLLSVDGVEALHSLHIWALTVAQPVLSVHIAIAQNVDAQAVLKVARDRLQGKFN 333 Query: 295 MHSLTIQMESPVDQDPDCLFCEDPCD 320 H++TIQ+ES + C C+ P + Sbjct: 334 FHTMTIQIESYSEDMKSCQECQGPSE 359 Pedant information for DKFZphfbr2_62f10, frame 2 Report for DKFZphfbr2_62f10.2 [LENGTH] 320 [MW] 35053.51 [pi] 6.48 [HOMOL] PIR:S70632 z c transporter ZnT-2 - rat 3e-84 [FUNCAT] 30.02 organization of plasma membrane [S. cerevisiae, YMR243c] 2e-16 [FUNCAT] 13.01 homeostasis of metal ions [S. cerevisiae, YMR243c] 2e-16 [FUNCAT] 08.19 cellular import [S. cerevisiae, YMR243c] 2e-16 , [FUNCAT] 11.07 detoxificaton [S. cerevisiae, YMR243c] 2e-16 [FUNCAT] 07.04.01 metal ion transporters (cu, fe, etc.) [S. cerevisiae, YMR243c] 2e-16 [FUNCAT] 08.04 mitochondrial transport [S. cerevisiae, YOR316c] 3e-13 [FUNCAT] 30.16 mitochondrial organization [Ξ. cerevisiae, YOR316c] 3e-13 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YDR205w] 4e-07 [PIRKW] transmembrane protein 2e-30 [PIRKW] mitochondrial inner membrane 6e-12 [PIRKW] mitochondrion 6e-12 [PIRKW] membrane protein le-11 [SUPFAM] zmc transporter ZnT-2 2e-30 [SUPFAM] membrane protein czcD le-11 [PROSITE] MYRISTYL 4 [PROSITE] CAMP_PHOSPHO_SITE 1 [PROSITE] CK2_PHOSPH0_SITE 1 [PROSITE] PR0KAR_LIP0PROTEIN 1 [PROSITE] TYR_PHOSPHO_SITE 1 [PROSITE] PKC_PHOSPHO_SITE 4 [PROSITE] ASN_GLYCOSYLATION 2 [KW] TRANSMEMBRANE 5 [KW] LOW COMPLEXITY 8.12 % SEQ MYHCHSGSKPTEKGANEYAYAKWKLCSASAICFIFMIAEVVGGHIAGSLAVVTDAAHLLI SEG xxx PRD cccccccccccccccchhhhhhhhhhhhhhhhhhhhhhhhcccchhhhhhhhhhhhhhhh MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMM SEQ DLTSFLLΞLFSLWLSSKPPSKRLTFGWHRAEILGALLΞILCIWVVTGVLVYLACERLLYP SEG xxxxxxxxxxxxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhccccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhc MEM MMMMMMMMMMMMM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM SEQ DYQIQATVMIIVSSCAVAANIVLTVVLHQRCLGHNHKEVQANASVRAAFVHAPGDLFQSI SEG PRD cccccccceeeehhhhhhhhhhhhhhhhhcccccccccccccchhhhhhhhhhhhhchhh MEM MMMMMMMMMMMMMMMMMMMMMMMMM MMMMMMMMMMMMMMMMMMMMM ... SEQ SVLISALIIYFKPEYKIADPICTFIFSILVLASTITILKDFSILLMEGVPKSLNYSGVKE SEG PRD hhhhhhhhhhcccceeeccchhhhhhhhhhhhhchhhhhhhheeeeeccccccchhhhhh MEM .. MMMMMMMMMMMMMMMMMMMM SEQ LILAVDGVLSVHCLHIWΞLTMNQVILSAHVATAASRDSQVVRREIAKALSKSFTMHSLTI SEG PRD hhhhhhceeecccceeeeeccchhhhheeeeeccccchhhhhhhhhhhhhhhhcccccee MEM SEQ QMESPVDQDPDCLFCEDPCD SEG PRD eeeccccccccccccccccc MEM Prosite for DKFZphfbr2_62f10.2 PS00001 162-5-166 ASN_GLYCOSYLATION PDOC00001 PS00001 234->238 ASN_GLYCOSYLATION PDOC00001 PS00004 81->85 CAMP_PHOSPHO_SITE PDOC00004 PS00005 11->14 PKC_PHOSPHO_SI E PDOC00005 PS00005 75->78 PKC PHOSPHO SITE PDOC00005 PS00005 80->83 PKC_PHOSPHO_SITE PDOC00005 PS00005 164->167 PKC_PHOSPHO_SITE PDOC00005 PS00006 304->308 CK2_PHOSPHO_SITE PDOC00006 PS00007 13->21 TYR_PHOSPHO_SITE PDOC00007 PS00008 7->13 MYRISTYL PDOC00008 PS00008 42->48 MYRISTYL PDOC00008 PS00008 94->100 MYRISTYL PDOC00008 PS00008 228->234 MYRISTYL PDOC00008 PS00013 125->136 PROKAR LIPOPROTEIN PDOC00013 (No Pfam data available for DKFZphfbr2_62f10.2) DKFZphfbr2 62nl0 group: brain derived DKFZphfbr2_62nlO encodes a novel 541 amino acid protein with similarity to Plasmodium vivax reticulocyte-binding protein 1. The novel protein contains one Leucine Zipper, involved in protem-protem-mteraction. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of brain-specific genes . similarity to reticulocyte-bind g protein complete cDNA, complete eds, EST hits Sequenced by LMU Locus: /map="13" Insert length: 3522 bp Poly A stretch at pos. 3503, polyadenylation signal at pos. 3479 1 GGGGCGTGTT GGCGGGATTC TGAACGCTGC CATGGCTCAG ACCGTGTAGA 51 ATGTTACATT GTCGCTCACT CTGCCCATCA CGTGCCACAT TTGCTTGGGG 101 AAGGTACGTC AGCCTGTCAT ATGCATCAAC AACCATGTAT TTTGTTCGAT 151 TTGTATTGAT TTGTGGTTGA AGAATAATAG CCAGTGTCCA GCTTGCAGAG 201 TCCCCATCAC TCCTGAAAAT CCTTGCAAAG AAATTATAGG AGGAACAAGT 251 GAAAGTGAAC CTATGCTAAG CCATACGGTC AGGAAGCATC TTCGGAAAAC 301 TAGACTTGAA TTACTACACA AAGAATATGA GGACGAAATA GATTGTTTAC 351 AGAAAGAAGT AGAAGAGCTT AAGAGTAAAA ATCTCAGCTT GGAGTCACAG 401 ATCAAAGCTA TTCTGGATCC TTTAACCTTG GTGCAGGGCA ACCAAAATGA 451 AGACAAACAT CTAGTCACAG ATAATCCAAG TATAATTAAC CCAGAAACTG 501 TAGCAGAGTG GAAGAAAAAA CTCAGAACAG CTAATGAAAT CTATGAAAAA 551 GTGAAAGATG ATGTGGATAA GCTAAAGGAG GCAAATAAAA AATTGAAATT 601 GGAAAATGGT GGTCTGGTGA GGGAGAATTT ACGACTGAAG GCTGAAGTTG 651 ATAACAGATC ACCTCAAAAG TTTGGAAGGT TTGCAGTTGC TGCTCTTCAG 701 TCCAAAGTAG AACAGTATGA GCGTGAAACC AATCGCCTCA AGAAAGCCCT 751 GGAACGAAGT GATAAGTATA TAGAGGAACT AGAATCTCAA GTTGCACAGC 801 TAAAAAATTC AAGTGAAGAG AAAGAGGCTA TGAATTCCAT TTGCCAGACA 851 GCACTTTCTG CAGATGGCAA AGGGAGCAAA GGCAGTGAGG AGGATGTGGT 901 GTCAAAGAAT CAAGGCGATA GTGCCAGAAA GCAGCCTGGC TCATCCACCT 951 CCAGTTCTTC TCACCTAGCG AAGCCTTCCA GCAGCAGACT GTGTGACACC 1001 AGTTCTGCAA GGCAGGAAAG TACCAGCAAA GCAGACCTTA ACTGTTCTAA 1051 GAACAAAGAC CTATATCAAG AACAGGTAGA AGTAATGTTA GATGTGACAG 1101 ATACAAGTAT GGATACTTAT TTGGAAAGAG AATGGGGGAA TAAACCAAGT 1151 GACTGTGTAC CCTACAAAGA TGAAGAACTT TATGATTTTC CAGCTCCTTG 1201 TACTCCTTTG TCCCTTAGTT GCCTTCAGCT CAGTACTCCA GAAAATAGAG 1251 AGAGCTCTGT GGTCCAAGCA GGAGGTTCCA AAAAGCACTC AAACCATCTC 1301 AGAAAATTGG TGTTTGATGA TTTTTGTGAT TCTTCAAATG TTTCTAATAA 1351 AGATTCTTCA GAAGATGATA TAAGTAGAAG TGAAAATGAG AAGAAATCAG 1401 AATGTTTTTC TTCCACAAAG ACAGGATTTT GGGACTGTTG TTCCACAAGC 1451 TATGCCCAAA ACTTAGATTT TGAAAGTTCA GAGGGGAACA CGATAGCAAA 1501 TTCTGTTGGA GAAATATCTT CAAAATTGAG TGAGAAATCA GGCTTATGTT 1551 TATCCAAAAG GTTGAATTCT ATTCGCTCTT TTGAAATGAA CCGGACAAGA 1601 ACATCCAGTG AAGCATCGAT GGATGCTGCT TACCTTGACA AAATCTCTGA 1651 GTTGGATTCA ATGATGTCAG AGTCAGACAA CAGCAAGAGC CCTTGTAATA 1701 ACGGTTTTAA GTCACTGGAT TTGGATGGGT TATCAAAGTC ATCTCAAGGC 1751 AGTGAATTTC TTGAGGAACC TGATAAGTTG GAAGAAAAAA CTGAGCTAAA 1801 CCTTTCCAAA GGTTCTCTAA CTAATGATCA GTTAGAAAAT GGAAGTGAAT 1851 GGAAACCCAC TTCTTTTTTT TCTCCTCTCT CCATCTGACC AAGAAATGAA 1901 TGAAGATTTT TCACTCCATT CCAGTTCTTG TCCAGTAACT AATGAAATCA 1951 AACCCCCAAG CTGCTTGTTT CAGACAGAGT TTTCCCAGGG CATTTTGTTA 2001 AGCAGTTCAC ATCGACTATT GGAAGATCAA AGATTTGGGT CATCTTTGTT 2051 TAAGATGTCC TCAGAGATGC ACAGTCTTCA TAACCACCTT CAGTCTCCTT 2101 GGTCTACTTC CTTTGTGCCT GAAAAGAGGA ATAAAAATGT GAATCAATCA 2151 ACAAAAAGAA AAATCCAGAG CAGCCTTTCC AGTGCCAGCC CATCAAAAGC 2201 AACTAAAAGT TGACTCATTA GAAAGGTGTC ATTTGTGGTT TTGTCCTGAG 2251 AGAAATAGAA AAGTTGTTAA AGTTACCTTT TTTCCTCATA AAAGTTCTAT 2301 ACAAATTGGA ATTGATAATC TTTAGTCAAG TATCAAGTCA GGATGGTGGA 2351 TTAACCTGTA CCCAGAATAC TTATTGTTCA TTTTGAAAAG ACTTTGTTCT 2401 TTTCATTTTT ATTTGGGAGT CTTTGTGACC AGAGAAGTTA GGGAGGAGGT 2451 TATTTTTGTG TTTTGGGGTT GGTTGGTTGG TTGGTTTTGT TTTTGGTTTT 2501 GTTTTTTTAC TGAATTTGAT ATGTATCTCG GTTGGATATA CATTGTTTTT 2551 TTAAAAAATG TTATTTAACT GTTAGATACA GTGGCCTGTT GATAAGCCCC 2601 ACTTGTCTTC AGAACTTGGA TTTCTTAAAT AAAACTTTTA GTGTTGTCTA 2651 TACACTGCTC AATAAGACAC TTGAGTTTAA GCTTTTCCCA GGGTGGAAAT 2701 TATTTTACCT GTCCCTTTTT ATTTATGTTT AGTGATGGCC TAGTTTTTCT 2751 GCAGGGCCAT GATGGAGAAA TAGCACTCTA GCCTTAGTCC AATATTGATT 2801 TACTTTCTTT TTTTAGGTTT TATGTATATG TTTGCATTTT TTAGCATTGT 2851 GTTTTGTCCA GTTTTGTGAA AATGTTCTGC TAGTATGAAA GAAAACATTT 2901 TCTATATGAA GACATTTGTT TTATGTTAGG TAGCTTACAT TTTCTCCTCT 2951 GCGTGTGTGT GTATGTGTGT AAAATCAGAA ATTTAGCATA CTATGGAAAG 3001 AAGGCATGGA GCACTTGGGT TTAGAGGAAC CTAAAACATC ATAGCTTCAT 3051 TGTTCCAGAT GTAACAGGTT TGAAAGAGCT CATCGCCAAG TTCTTGATCC 3101 ACTTGCATTC CAGGGGAGTT CTCTTTTGAG TAGTATGTTT CTTGTTTGCA 3151 TGTTCCTGTT CTTTGTGGAA ACTATGCATG GTAGCATTTT TGCTTGCTGT 3201 GTTTTCCATA CTTAAGAAAA AGAGGTTTCA GTTGGCTGAT AGAATATCTT 3251 TTATGTAGGA CAAAACTTTT CTGTGAAGAG TGTTGAGGGG GTGAAGATAG 3301 GTAAGAGGTA AGCACAATTT TTAATTTAGG CTCTGAAAAA GTGTATTGTT 3351 CTAAACGTAT TTGGTATGCC TATATAGGTC TTTAAAAATG GGTTTGTATG 3401 CTGTTTAATG TGCACTGAAC ATTTTACATT AATATTGTAC TGTTTTACAT 3451 TAATACTGCA TGCTTTTCTA TGTGAATTGA ATAAAGAATG TCATAAGCAC 3501 TGGAAAAAAA AAAAAAAAAA AA BLAST Results Entry HSΘ58254 from database EMBL: human STS SHGC-11774. Score = 1643, P = 8.0e-67, identities = 345/355 Entry HS513217 from database EMBL: human STS SHGC-14656. Score = 1193, P = 5.8e-46, identities = 241/244 Medline entries No Medline entry Peptide information for frame 2 ORF from 263 bp to 1885 bp; peptide length: 541 Category: similarity to known protein 1 MLSHTVRKHL RKTRLELLHK EYEDEIDCLQ KEVEELKSKN LSLESQIKAI 51 LDPLTLVQGN QNEDKHLVTD NPSIINPETV AEWKKKLRTA NEIYEKVKDD 101 VDKLKEANKK LKLENGGLVR ENLRLKAEVD NRSPQKFGRF AVAALQSKVE 151 QYERETNRLK KALERSDKYI EELESQVAQL KNSSEEKEAM NSICQTALSA 201 DGKGSKGSEE DVVSKNQGDS ARKQPGSSTS SSSHLAKPSS SRLCDTSSAR 251 QESTSKADLN CSKNKDLYQE QVEVMLDVTD TΞMDTYLERE WGNKPSDCVP 301 YKDEELYDFP APCTPLSLSC LQLSTPENRE SSVVQAGGSK KHSNHLRKLV 351 FDDFCDSSNV SNKDSSEDDI SRSENEKKSE CFSSTKTGFW DCCSTSYAQN 401 LDFESSEGNT IANSVGEISS KLSEKSGLCL SKRLNSIRSF EMNRTRTSSE 451 ASMDAAYLDK ISELDSMMSE SDNSKSPCNN GFKSLDLDGL SKSSQGSEFL 501 EEPDKLEEKT ELNLSKGSLT NDQLENGSEW KPTSFFSPLS I BLASTP hits Entry A42771 from database PIR: reticulocyte-binding protein 1 - Plasmodium vivax Score = 127, P = 3.7e-08, identities = 68/300, positives = 145/300 Entry RBP1_PLAVB from database SWISSPROT: RETICULOCYTE BINDING PROTEIN 1 PRECURSOR. Score = 127, P = 3.9e-08, identities = 68/300, positives = 145/300 Entry MMDSPPG_1 from database TREMBL: gene: "DSPP"; product: "dentin sialophosphoprotein"; Mus musculus DSPP gene Score = 160, P = 5.2e-08, identities = 87/373, positives = 146/373 Alert BLASTP hits for DKFZphfbr2_62nlO, frame 2 No Alert BLASTP hits found Pedant information for DKFZphfbr2_62nlO, frame 2 Report for DKFZphfbr2_62nlO .2 [LENGTH] 541 [MW] 60533.06 [pi] 5.10 [FUNCAT] 04.99 other transcription activities [S. cerevisiae, YKR092c] 3e-05 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YKR092c] 3e-05 [PROSITE] LEUCINE ZIPPER 1 [PROSITE] MYRISTYL 7 [PROSITE] CAMP PHOSPHO SITE 1 [PROSITE] CK2 PHOSPHO SITE 18 [PROSITE] PROKAR LIPOPROTEIN 1 [PROSITE] TYR PHOSPHO SITE 1 [PROSITE] PKC PHOSPHO SITE 14 [PROSITE] ASN GLYCOSYLATION 7 [KW] All Alpha [KW] LOW COMPLEXITY 9.24 % [KW] COILED COIL 22.55 % SEQ MLSHTVRKHLRKTRLELLHKEYEDEIDCLQKEVEELKSKNLSLESQIKAILDPLTLVQGN SEG PRD ccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccchhhhhhhcccccccccc COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC SEQ QNEDKHLVTDNPSI INPETVAEWKKKLRTANEI EKVKDDVDKLKEANKKLKLENGGLVR SEG xxxxxxxxxxxxxxxxxxxx PRD cccceeeeeccccccccchhhhhhhhhhhhhhhhhhhhchhhhhhhhhhhhhhcccceee COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC SEQ ENLRLKAEVDNRSPQKFGRFAVAALQSKVEQYERETNRLKKALERSDKYIEELESQVAQL SEG PRD ehhhhhhhhcccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC SEQ KNSSEEKEAMNSICQTALSADGKGSKGSEEDVVSKNQGDSARKQPGSSTSSSSHLAKPSS SEG xxxxxxxxxxxxxx PRD hcchhhhhhhhhhhhhhhccccccccccceeeeecccccccccccccccccccccccccc COILS CCCCCC SEQ SRLCDTSSARQESTSKADLNCSKNKDLYQEQVEVMLDVTDTSMDTYLEREWGNKPSDCVP SEG x PRD ccccccccccccccccccccccccchhhhhhhhhcccccccccchhhhhhhccccccccc COILS SEQ YKDEELYDFPAPCTPLSLSCLQLSTPENRESSVVQAGGSKKHSNHLRKLVFDDFCDSSNV SEG PRD cccccccccccccccccceeeecccccccceeeeeccccccccccccccccccccccccc COILS SEQ SNKDSSEDDISRΞENEKKSECFSSTKTGFWDCCSTSYAQNLDFESSEGNTIANSVGEISS SEG PRD cccccccchhhhhccccccccccccccccccccccccccccccccccccccccccccccc COILS SEQ KLSEKSGLCLSKRLNSIRSFEMNRTRTSSEASMDAAYLDKISELDSMMSESDNSKSPCNN SEG PRD ccccccccchhhhhcccccccccccchhhhhhhhhhhhhhhhhccccccccccccccccc COILS SEQ GFKSLDLDGLSKSSQGSEFLEEPDKLEEKTELNLSKGSLTNDQLENGSEWKPTSFFSPLS SEG .. xxxxxxxxxxxxxxx PRD ccccccccccccccccceeecccchhhhhhhhhccccccccccccccccccccccccccc COILS SEQ SEG PRD COILS Prosite for DKFZphfbr2_62nlO .2 PS00001 40->44 ASN_GLYCOSYLATION PDOC00001 PS00001 182->186 ASN_GLYCOSYLATION PDOC00001 PS00001 260->264 ASN GLYCOSYLATION PDOC00001 PS00001 359->363 ASN_GLYCOSYLATION PDOC00001 PS00001 43->447 ASN_GLYCOSYLATION PDOC00001 PS00001 513->517 ASN_GLYCOSYLATION PDOC00001 PS00001 526->530 ASN_GLYCOSYLATION PDOC00001 PS00004 340->344 CAMP_PHOSPHO_SITE PDOC00004 PS00005 5->8 PKC_PHOSPHO_SITE PDOC00005 PS00005 156->159 PKC_PHOSPHO_SITE PDOC00005 PS00005 166->169 PKC_PHOSPHO_SITE PDOC00005 PS00005 220->223 PKC_PHOSPHO_SITE PDOC00005 PS00005 240->243 PKC_PHOSPHO_SITE PDOC00005 PS00005 248->251 PKC_PHOSPHO_SITE PDOC00005 PS00005 254->257 PKC_PHOSPHO_SITE PDOC00005 PS00005 339->342 PKC_PHOSPHO_SITE PDOC00005 PS00005 361->364 PKC_PHOSPHO_SITE PDOC00005 PS00005 384->387 PKC_PHOSPHO_SITE PDOC00005 PS00005 419->422 PKC_PHOSPHO_SITE PDOC00005 PS00005 423->426 PKC_PHOSPHO_SITE PDOC00005 PS00005 431->434 PKC_PHOSPHO_SITE PDOC00005 PS00005 436->439 PKC_PHOSPHO_SITE PDOC00005 PS00006 13->17 CK2_PHOSPHO_SITE PDOC00006 PS00006 79->83 CK2_PHOSPHO_SITE PDOC00006 PS00006 89->93 CK2_PHOSPHO_SITE PDOC00006 PS00006 147->151 CK2_PHOSPHO_SITE PDOC00006 PS00006 183->187 CK2_PHOSPHO_SITE PDOC00006 PS00006 208->212 CK2_PHOSPHO_SITE PDOC00006 PS00006 255->259 CK2_PHOSPHO_SITE PDOC00006 PΞ00006 281->285 CK2_PHOSPHO_SITE PDOC00006 PS00006 285->289 CK2_PHOSPHO_SITE PDOC00006 PS00006 324->328 CK2_PHOSPHO_SITE PDOC00006 PS0O006 361->365 CK2_PHOSPHO_SITE PDOC00006 PS00006 365->369 CK2_PHOSPHO_SITE PDOC00006 PS00006 371->375 CK2_PHOSPHO_SITE PDOC00006 PS00006 373->377 CK2_PHOSPHO_SITE PDOC00006 PS0O006 414->418 CK2_PHOSPHO_SITE PDOC00006 PS00006 447->451 CK2_PHOSPHO_SITE PDOC00006 PS00006 462->466 CK2_PHOSPHO_SITE PDOC00006 PS00006 469->473 CK2_PHOSPHO_SITE PDOC00006 PS00007 294->302 TYR_PHOSPHO_SITE PDOC00007 PS00008 204->210 MYRISTYL PDOC00008 PS00008 226->232 MYRISTYL PDOC00008 PS00008 292->298 MYRISTYL PDOC00008 PS00008 408->414 MYRISTYL PDOC00008 PS00008 427->433 MYRISTYL PDOC00008 PS00008 489->495 MYRISTYL PDOC00008 PS0O008 517->523 MYRISTYL PDOC00008 PS00013 310->321 PROKAR_LIPOPROTEIN PDOC00013 PS00029 104->126 LEUCINE ZIPPER PDOC00029 (No Pfam data available for DKFZphfbr2_62nlO .2) DKFZphfbr2 62ol7 group: metabolism DKFZphfbr2_62ol7.2 encodes a novel 282 ammo acid protein with weak similarity to the apolipoprotein E receptor. The new protein contains a leucine zipper for protein-protein interaction, and three LDL- receptor class A domain (LDLRA_1) patterns. In LDL-receptors the class A domains form the binding site for LDL and calcium. The acidic residues between the fourth and sixth cysteines are important for high-affinity binding of positively charged sequences in LDLR's ligands. The new protein can find application m modulation of cholesterol binding and transport by LDL-receptors and LDL-bmding proteins similarity to apolipoprotein E receptor complete cDNA, complete eds, start at Bp 56 matches kozak consensus ANCatg EST hits Sequenced by LMU Locus : unknown Insert length: 1260 bp Poly A stretch at pos. 1240, polyadenylation signal at pos. 1218 1 GGGGGATAAG AGAGCGGTCT GGACAGCGCG TGGCCGGCGC CGCTGTGGGG 51 ACAGCATGAG CGGCGGTTGG ATGGCGCAGG TTGGAGCGTG GCGAACAGGG 101 GCTCTGGGCC TGGCGCTGCT GCTGCTGCTC GGCCTCGGAC TAGGCCTGGA 151 GGCCGCCGCG AGCCCGCTTT CCACCCCGAC CTCTGCCCAG GCCGCAGGCC 201 CCAGCTCAGG CTCGTGCCCA CCCACCAAGT TCCAGTGCCG CACCAGTGGC 251 TTATGCGTGC CCCTCACCTG GCGCTGCGAC AGGGACTTGG ACTGCAGCGA 301 TGGCAGCGAT GAGGAGGAGT GCAGGATTGA GCCATGTACC CAGAAAGGGC 351 AATGCCCACC GCCCCCTGGC CTCCCCTGCC CCTGCACCGG CGTCAGTGAC 401 TGCTCTGGGG GAACTGACAA GAAACTGCGC AACTGCAGCC GCCTGGCCTG 451 CCTAGCAGGC GAGCTCCGTT GCACGCTGAG CGATGACTGC ATTCCACTCA 501 CGTGGCGCTG CGACGGCCAC CCAGACTGTC CCGACTCCAG CGACGAGCTC 551 GGCTGTGGAA CCAATGAGAT CCTCCCGGAA GGGGATGCCA CAACCATGGG 601 GCCCCCTGTG ACCCTGGAGA GCGTCACCTC TCTCAGGAAT GCCACAACCA 651 TGGGGCCCCC TGTGACCCTG GAGAGTGTCC CCTCTGTCGG GAATGCCACA 701 TCCTCCTCTG CCGGAGACCA GTCTGGAAGC CCAACTGCCT ATGGGGTTAT 751 TGCAGCTGCT GCGGTGCTCA GTGCAAGCCT GGTCACCGCC ACCCTCCTCC 801 TTTTGTCCTG GCTCCGAGCC CAGGAGCGCC TCCGCCCACT GGGGTTACTG 851 GTGGCCATGA AGGAGTCCCT GCTGCTGTCA GAACAGAAGA CCTCGCTGCC 901 CTGAGGACAA GCACTTGCCA CCACCGTCAC TCAGCCCTGG GCGTAGCCGG 951 ACAGGAGGAG AGCAGTGATG CGGATGGGTA CCCGGGCACA CCAGCCCTCA 1001 GAGACCTGAG CTCTTCTGGC CACGTGGAAC CTCGAACCCG AGCTCCTGCA 1051 GAAGTGGCCC TGGAGATTGA GGGTCCCTGG ACACTCCCTA TGGAGATCCG 1101 GGGAGCTAGG ATGGGGAACC TGCCACAGCC AGAACCGAGG GGCTGGCCCC 1151 AGGCAGCTCC CAGGGGGTAG GACGGCCCTG TGCTTAAGAC ACTCCTGCTG 1201 CCCCGTCTGA GGGTGGCGAT TAAAGTTGCT TCACATCCTC AAAAAAAAAA 1251 AAAAAAAAAC BLAST Results No BLAST result Medlme entries No Medline entry Peptide information for frame 2 ORF from 56 bp to 901 bp; peptide length: 282 Category: similarity to known protein Classi ication: unset Prosite motifs: LDLRA_1 (67-90) LDLRA_1 ( 67 -90 ) LDLRA 1 ( 145-168 ) LEUCINE ZIPPER (17-39) 1 MSGGWMAQVG AWRTGALGLA LLLLLGLGLG LEAAASPLST PTSAQAAGPS 51 SGSCPPTKFQ CRTSGLCVPL TWRCDRDLDC SDGSDEEECR IEPCTQKGQC 101 PPPPGLPCPC TGVSDCSGGT DKKLRNCSRL ACLAGELRCT LSDDCIPLTW 151 RCDGHPDCPD SSDELGCGTN EILPEGDATT MGPPVTLESV TSLRNATTMG 201 PPVTLESVPS VGNATSSΞAG DQSGSPTAYG VIAAAAVLSA SLVTATLLLL 251 SWLRAQERLR PLGLLVAMKE SLLLSEQKTS LP BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_62ol7, frame 2 TREMBL:AF110520_6 product: "NG29"; Mus musculus major histocompatibility complex region NG27, NG28, RPS28, NADH oxidoreductase, NG29, KIFCl, Fas-binding protein, BINGl, tapasin, RalGDS-like, KE2, BING4, beta 1, 3-galactosyl transferase, and RPS18 genes, complete eds; Sacm21 gene, partial eds; and unknown gene., N 1, Score = 733, P = 1.5e-72 PIR:JE0237 apolipoprotein E receptor 2 precursor mouse, N = 2, Score = 290, P = l.le-26 TREMBL:HSZ75190_1 product: "apolipoprotein E receptor 2 906"; H. sapiens mRNA for apolipoprotein E receptor 2, N = 1, Score = 279, P = 1.8e-23 >TREMBL:AF110520_6 product: "NG29"; Mus musculus major histocompatibility complex region NG27, NG28, RPS28, NADH oxidoreductase, NG29, KIFCl, Fas-bindmg protein, BINGl, tapasin, RalGDS-like, KE2, BING4, beta 1, 3-galactosyl transferase, and RPS18 genes, complete eds; Sacra21 gene, partial eds; and unknown gene. Length = 260 HSPs: Score = 733 (110.0 bits) , Expect = 1.5e-72, P = 1.5e-72 Identities = 157/276 (56 ! I, Positives = 178/276 (64%) Query: 6 MAQVGAWRTGALGLALLLLLGLGLGLEAAASPLSTPTSAQAAGPSSGSCPPTKFQCRTSG 65 MA+ GA R ALGL L LL GL GLEAA +P T Q +G + SCP FQC TSG Sbjct: 1 MARGGAGRAVALGLVLRLLFGLRTGLEAAPAPAHT—RVQVSGSRADSCPTDTFQCLTSG 58 Query: 66 LCVPLTWRCDRDLDCSDGSDEEECRIEPCTQKGQCPPPPGLPCPCTGVSDCSGGTDKKLR 125 CVPL+WRCD D DCSDGSDEE+CRIE C Q GQC P LPC C +S CS +DK L Sbjct: 59 YCVPLSWRCDGDQDCSDGSDEEDCRIESCAQNGQCQPQSALPCSCDNISGCSDVSDKNL- 117 Query: 126 NCSRLACLAGELRCTLSDDCIPLTWRCDGHPDCPDSSDELGCGTNEILPEGDATTMGPPV 185 NCSR C EL C L D CIP TWRCDGHPDC DSSDEL C T+ Sbjct: 118 NCSRPPCQESELHCILDDVCIPHTWRCDGHPDCLDSSDELSCDTD T 163 Query: 186 TLESVTSLRNATTMGPPVTLESVPSVGNATSSSAGDQSGSPTAYGVIAAAAVLSASLVTA 245 ++ + NATT T+E+ S N T +SAGD S +P+AYGVIAAA VLSA LV+A Sbjct: 164 EIDKIFQEENATTTRISTTMENETSFRNVTFTSAGDSSRNPSAYGVIAAAGVLSAILVSA 223 Query: 246 TLLLLSWLRAQERLRPLGLLVAMKESLLLSEQKTSL 281 TLL+L LR Q L P GLLVA+KESLLLSE+KTSL Sbjct: 224 TLLILLRLRGQGYLPPPGLLVAVKESLLLSERKTSL 259 Pedant information for DKFZphfbr2_62ol7, frame 2 Report for DKFZphfbr2_62ol7.2 [LENGTH] 282 [MW] 28991.19 [pi] 4.61 [HOMOL] TREMBL :AF110520_6 product: "NG29"; Mus musculus major histocompatibility complex region NG27, NG28, RPS28, NADH oxidoreductase, NG29, KIFCl, Fas-binding protein, BINGl, tapasin, RalGDS-like, KE2, BING4, beta 1, 3-galactosyl transferase, and RPS18 genes, complete eds; Sacm21 gene, partial eds; and unknown gene. 5e-55 [BLOCKS] BL01209 LDL-receptor class A (LDLRA) domain proteins [SCOP] dlaj 7.11.1.1.1 Ligand-binding domain of low-density lipoprotei 2e-10 [PIRKW] duplication le-19 ['PIRKW] tandem repeat le-15 [PIRKW] heterodimer 6e-18 [PIRKW] endocytosis 4e-18 [PIRKW] heparan sulfate 2e-12 [PIRKW] VLDL le-19 [PIRKW] transmembrane protein le-19 [PIRKW] coated pits 4e-18 [PIRKW] fatty acid metabolism le-19 [PIRKW] G protein-coupled receptor le-10 [PIRKW] receptor le-19 [PIRKW] glycoprotein le-19 [PIRKW] lipid transport 4e-18 [PIRKW] LDL 5e-14 [PIRKW] calcium binding 6e-18 [PIRKW] extracellular protein 6e-13 [PIRKW] alternative splicing le-19 [PIRKW] extracellular matrix 3e-10 [PIRKW] chondroit sulfate proteoglycan 2e-12 [PIRKW] cholesterol 4e-18 [SUPFAM] leucine-rich alpha-2-glycoproteιn repeat homology le-10 [SUPFAM] LDL receptor YWTD-containing repeat homology le-19 [SUPFAM] trypsin homology 6e-13 [SUPFAM] alpha-2-macroglobulιn receptor 6e-18 [SUPFAM] LDL receptor le-19 [SUPFAM] LDL receptor ligand-binding repeat homology le-19 [SUPFAM] EGF homology le-19 [PROSITE] LDLRA_13 [PROSITE] LEUCINE_ZIPPER 1 [PFAM] Low-density lipoprotein receptor domain class A [PFAM] TNFR/NGFR cysteine-rich region [KW] SIGNAL_PEPTIDE 31 [KW] TRANSMEMBRANE 1 [KW] LOW_COMPLEXITY 22.34 % SEQ MSGGWMAQVGAWRTGALGLALLLLLGLGLGLEAAASPLSTPTSAQAAGPSSGSCPPTKFQ SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD cccccccccccchhhhhhhhhhhhhhhhhhhhhhhhccccccccccccccccccccceee MEM SEQ CRTSGLCVPLTWRCDRDLDCSDGSDEEECRIEPCTQKGQCPPPPGLPCPCTGVSDCSGGT SEG xxxxxxxxxxx PRD ecccccceeeeecccccccccccccccccccccccccccccccccccccccccccccccc MEM SEQ DKKLRNCSRLACLAGELRCTLSDDCIPLTWRCDGHPDCPDSSDELGCGTNEILPEGDATT SEG PRD cccccccccccccccceeeccccccccccccccccccccccccccccccccccccccccc MEM SEQ MGPPVTLESVTSLRNATTMGPPVTLESVPSVGNATSSSAGDQSGSPTAYGVIAAAAVLSA SEG xxxxxxxx PRD ccccccccccccccccccccccccccccccccccccccccccccccchhhhhhhhhhhhh MEM MMMMMMM SEQ SLVTATLLLLSWLRAQERLRPLGLLVAMKESLLLSEQKTSLP SEG xxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhhcccchhhhhhhhhhhhhcccccc MEM MMMMMMMMMM Prosite for DKFZphfbr2_62ol7.2 PS01209 67->90 LDLRA_1 PDOC00929 PΞ01209 67->90 LDLRA_1 PDOC00929 PS01209 145->168 LDLRA_1 PDOC00929 PS00029 17->39 LEUCINE ZIPPER PDOC00029 Pfam for DKFZphfbr2_62ol7.2 HMM_NAME TNFR/NGFR cysteine-rich region HMM *CpeGtYtD.WNHvpqClpC.trCePEMGQYMvqPCTwTQNT.VC* CP+ ++ + + C+P RC+ ++ +C + ++ +C Query 54 CPPTKFQCRTS--GLCVPLTWRCDR—DL DCSDGSDEEEC 89 HMM_NAME Low-density lipoprotein receptor domain class A HMM *tTCeGPDEFQCgSGeMRCIPMsWvCDGDpDCeDWSDEWPeNChp* C P +FQC+++ C+P+ W+CD D DC D+SDE E+C+ Query 52 GSCP-PTKFQCRTSG-LCVPLTWRCDRDLDCSDGSDE—EECRI 91 54.99 (bits) f: 130 t: 169 Target: dkfzphfbr2_62ol7.2 similarity to apolipoprotein E receptor Alignment to HMM consensus: Query *tTCeGPDEFQCgSGeMRCIPMsWvCDGDpDCeDWSDEWPeNChp* C + E +C + CIP+ W+CDG PDC D SDE ++C+ dkfzphfbr2 130 LACL-AGELRCTLΞD-DCIPLTWRCDGHPDCPDSSDE—LGCGT 169 DKFZphfbr2_64al5 group: nucleic acid management DKFZphfbr2_64al5 encodes a novel 255 amino acid protein with strong similarity to inorganic pyrophosphatases Inorganic pyrophosphatase (EC 3.6.1.1) (PPase) is the enzyme responsible for the hydrolysis of pyrophosphate (PPi) which is formed as the product of the many biosynthetic reactions that utilize ATP. All known PPases require the presence of divalent metal cations, with magnesium conferring the highest activity. The new protein can find application as a new enzyme for biotechnologic processes. strong similarity to inorganic pyrophosphatases unspliced Intron 212-256 see EST HS1190948 Sequenced by Qiagen Locus : unknown Insert length: 1188 bp Poly A stretch at pos. 1170, polyadenylation signal at pos. 1151 1 GGGGGTTGGG GACCAGTGCA GGGACCGGGT CGCGCCGTGC TATGGCCCTG 51 TACCACACTG AGGAGCGCGG CCAGCCCTGC TCGCAGAATT ACCGCCTCTT 101 CTTTAAGAAT GTAACTGGTC ACTACATTTC CCCCTTTCAT GATATTCCTC 151 TGAAGGTGAA CTCTAAAGAG GACACTGAGG CTCAAGGCAT TTTTATAGAC 201 TTGTCTAAGA TCTGGAAAAT GGCATTCCTA TGAAGAAAGC ACGAAATGAT 251 GAATATGAGA ATCTGTTTAA TATGATTGTA GAAATACCTC GGTGGACAAA 301 GGCTAAAATG GAGATTGCCA CCAAGGAGCC AATGAATCCC ATTAAACAAT 351 ATGTAAAGGA TGGAAAGCTA CGCTATGTGG CGAATATCTT CCCTTACAAG 401 GGTTATATAT GGAATTATGG TACCCTCCCT CAGACTTGGG AAGATCCCCA 451 TGAAAAAGAT AAGAGCACGA ACTGCTTTGG AGATAATGAT CCTATTGATG 501 TTTGCGAAAT AGGCTCAAAG ATTCTTTCTT GTGGAGAAGT TATTCATGTG 551 AAGATCCTTG GAATTTTGGC TCTTATTGAT GAAGGTGAAA CAGATTGGAA 601 ATTAATTGCT ATCAATGCGA ATGATCCTGA AGCCTCAAAG TTTCATGATA 651 TTGATGATGT TAAGAAGTTC AAACCGGGTT ACCTGGAAGC TACTCTTAAT 701 TGGTTTAGAT TATGTAAGGT ACCAGATGGA AAACCAGAAA ACCAGTTTGC 751 TTTTAATGGA GAATTCAAAA ACAAGGCTTT TGCTCTTGAA GTTATTAAAT 801 CCACTCATCA ATGTTGGAAA GCATTGCTTA TGAAGAACTG TAATGGAGGA 851 GCTACAAATT GCACAAACGT GCAGATATCT GATAGCCCTT TCCGTTGCAC 901 TCAAGAGGAA GCAAGATCAT TAGTTGAATC GGTATCATCT TCACCAAATA 951 AAGAAAGTAA TGAAGAAGAG CAAGTGTGGC ACTTCCTTGG CAAGTGATTG 1001 AAACATCTGA AATTCTGCTG TCAAGATTCC CATCTCTAAG GACTCCAAGA 1051 CTCTTTTTCC CCAAGTGCTA GAGACAAGGG GGTCTATGAG CATTTACTGA 1101 CTTCCTGTTA AAACTTCATT TTTTCAAACT TTTTGAGCTA TGCAATATAT 1151 AAATAAACAG TAAGAATTTT AAAAAAAAAA AAAAAAAA BLAST Results Entry HSPPASEMR from database EMBL: H. sapiens partial mRNA for pyrophosphatase. Score = 1706, P = 1.6e-70, identities = 342/343 Medlme entries No Medline entry Peptide information for frame 2 ORF from 230 bp to 994 bp; peptide length: 255 Category: strong similarity to known protein Classification: unset Prosite motifs: PPASE (85-92) 1 MKKARNDEYE NLFNMIVEIP RWTKAKMEIA TKEPMNPIKQ YVKDGKLRYV 51 ANIFPYKGYI WNYGTLPQTW EDPHEKDKST NCFGDNDPID VCEIGSKILS 101 CGEVIHVKIL GILALIDEGE TDWKLIAINA NDPEASKFHD IDDVKKFKPG 151 YLEATLNWFR LCKVPDGKPE NQFAFNGEFK NKAFALEVIK STHQCWKALL 201 MKNCNGGATN CTNVQISDSP FRCTQEEARS LVEΞVSSSPN KESNEEEQVW 251 HFLGK BLASTP hits Entry IPYR_KLULA from database SWISSPROT: INORGANIC PYROPHOSPHATASE (EC 3.6.1.1) (PYROPHOSPHATE PHOSPHO- HYDROLASE) (PPASE) . Score = 689, P = 6.0e-68, identities = 128/248, positives = 170/248 Entry A45153 from database PIR: inorganic pyrophosphatase (EC 3.6.1.1) - bovine Score = 862, P = 2.8e-86, identities = 146/226, positives = 190/226 Entry AF085600_1 from database TREMBLNEW: gene: "Nurf-38"; product: "inorganic pyrophosphatase NURF-38"; Drosophila melanogaster inorganic pyrophosphatase NURF-38 (Nurf-38) gene, complete eds. Score = 731, P = 2.1e-72, identities = 134/248, positives = 177/248 Entry PWBY from database PIR: inorganic pyrophosphatase (EC 3.6.1.1) - yeast (Saccharomyces cerevisiae) Score = 688, P = 7.7e-68, identities = 133/251, positives = 174/251 Alert BLASTP hits for DKFZphfbr2_64al5, frame 2 SWISSPROT: I PYR_DROME INORGANIC PYROPHOSPHATASE (EC 3.6.1.1) (PYROPHOSPHATE PHOSPHO- HYDROLASE) (PPASE)., N = 1, Score = 731, P = 2.4e-72 >SWISSPROT:IPYR_DROME INORGANIC PYROPHOSPHATASE (EC 3.6.1.1) (PYROPHOSPHATE PHOSPHO- HYDROLASE) (PPASE) . Length = 290 HSPs: Score = 731 (109.7 bits), Expect = 2.4e-72, P = 2.4e-72 Identities = 134/248 (54%), Positives = 177/248 (71%) Query: 7 DEYENLFNMIVEIPRWTKAKMEIATKEPMNPIKQYVKDGKLRYVANIFPYKGYIWNYGTL 66 +E + ++NM+VE+PRWT AKMEI+ K PMNPIKQ +K GKLR+VAN FP+KGYIWNYG L Sbjct: 40 NEEKTIYNMVVEVPRWTNAKMEISLKTPMNPIKQDIKKGKLRFVANCFPHKGYIWNYGAL 99 Query: 67 PQTWEDPHEKDKSTNCFGDNDPIDVCEIGSKILSCGEVIHVKILGILALIDEGETDWKLI 126 PQTWE+P + ST C GDNDPIDV EIG ++ G+V+ VK+LG ALIDEGETDWK+I Sbjct: 100 PQTWENPDHIEPSTGCKGDNDPIDVIEIGYRVAKRGDVLKVKVLGQFALIDEGETDWKII 159 Query: 127 AINANDPEASKFHDIDDVKKFKPGYLEATLNWFRLCKVPDGKPENQFAFNGEFKNKAFAL 186 AI+ NDP ASK +DI DV ++ PG L AT+ WF++ K+PDGKPENQFAFNG+ KN FA Sbjct: 160 AIDVNDPLASKVNDIADVDQYFPGLLRATVEWFKIYKIPDGKPENQFAFNGDAKNADFAN 219 Query: 187 EVIKSTHQCWKALLMKNCNGGATNCTNVQISDSPFRCTQEEARS-LVESVSSSPNKESNE 245 +1 TH+ W+ L+ ++ G+ + TN+ +S +EEA L E+ +E ++ Sbjct: 220 TIIAETHKFWQNLVHQSPASGSISTTNITNRNSEHVIPKEEAEKILAEAPDGGQVEEVSD 279 Query: 246 EEQVWHFL 253 WHF+ Sbjct: 280 TVDTWHFI 287 Peptide information for frame 3 ORF from 42 bp to 230 bp; peptide length: 63 Category: strong similarity to known protein Classification: unset 1 MALYHTEERG QPCSQNYRLF FKNVTGHYIS PFHDIPLKVN SKEDTEAQGI 51 FIDLSKIWKM AFL BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_64al5, frame 3 SWISSPROT :IPYR_DROME INORGANIC PYROPHOSPHATASE (EC 3.6.1.1) (PYROPHOSPHATE PHOSPHO- HYDROLASE) (PPASE)., N = 1, Score = 118, P = 8.8e-07 PIR:A45153 inorganic pyrophosphatase (EC 3.6.1.1) - bovine, N = 1, Score = 113, P = 3. le-06 TREMBLNEW:AF108211_1 product: "cytosolic inorganic pyrophosphatase"; Homo sapiens cytosolic inorganic pyrophosphatase mRNA, partial eds., N = 1, Score = 106, P = 1.8e-05 >SWISSPROT:IPYR_DROME INORGANIC PYROPHOSPHATASE (EC 3.6.1.1) (PYROPHOSPHATE PHOSPHO- HYDROLASE) (PPASE) . Length = 290 HSPs: Score = 118 (17.7 bits), Expect = 8.8e-07, P = 8.8e-07 Identities = 23/43 (53%), Positives = 29/43 (67%) Query: 1 MALYHTEERGQPCSQNYRLFFKNVTGHYISPFHDIPLKVNSKE 43 MALY T E+G Ξ +Y L+FKN G+ ISP HDIPL N ++ Sbjct: 1 MALYETVEKGAKNSPSYSLYFKNKCGNVISPMHDIPLYANEEK 43 Pedant information for DKFZphfbr2_64al5, frame 2 Report for DKFZphfbr2_64al5.2 [LENGTH] 255 [MW] 29177.34 [pi] 5.67 [HOMOL] TREMBLNEW: AF108211_1 product: "cytosolic inorganic pyrophosphatase"; Homo sapiens cytosolic inorganic pyrophosphatase mRNA, partial eds. 2e-93 [FUNCAT] 01.04.01 phosphate utilization [S. cerevisiae, YBROllc] 9e-73 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YBROllc] 9e-73 [FUNCAT] 02.99 other energy generation activities [S. cerevisiae, YMR267w] le-58 [FUNCAT] 30.16 mitochondrial organization [S. cerevisiae, YMR267w] le-58 [FUNCAT] 1 genome replication, transcription, recombination and repair [M. genitalium, MG351] le-06 [FUNCAT] g carbohydrate metabolism and transport [H. influenzae, HI0124] 2e-06 [BLOCKS] BL00387D [BLOCKS] BL00387C [BLOCKS] BL00387B [BLOCKS] BL00387A [SCOP] dlwgja_ 2.29.5.1.1 Inorganic pyrophosphatase [baker's yeas le-113 [EC] 3.6.1.1 Inorganic pyrophosphatase 7e-92 [PIRKW] mitochondrion 3e-57 [PIRKW] hydrolase 7e-92 [PIRKW] homodimer 2e-71 [SUPFAM] inorganic pyrophosphatase 7e-92 [PROSITE PPASE 1 [KW] Alpha_Beta [KW] 3D [KW] LOW COMPLEXITY 6.27 % SEQ MKKARNDEYENLFNMIVEIPRWTKAKMEIATKEPMNPIKQYVKDGKLRYVANIFPYKGYI SEG lhukB EGGGCEEEEEEEETTTbCBCEEETTTTTTTCEEECEETTEECBCCBBTTBTTbT SEQ WNYGTLPQTWEDPHEKDKSTNCFGDNDPIDVCEIGSKILSCGEVIHVKILGILALIDEGE SEG lhukB CEEEETTTTCBTTTTEETTTTEECCCBCCEEEECCCCCCTTTEEEEEEEEEEEEETTTTB SEQ TDWKLIAINANDPEASKFHDIDDVKKFKPGYLEATLNWFRLCKVPDGKPENQFAFNGEFK SEG lhukB CEEEEEEEETTTTTGGGCCCHHHHHHHTTTHHHHHHHHHHHHCGGGCCCCCCBCGGGCCB SEQ NKAFALEVIKSTHQCWKALLMKNCNGGATNCTNVQISDSPFRCTQEEARSLVESVSSSPN SEG xxxxxxxxx lhukB CHHHHHHHHHHHHHHHHHHHHCTTTTTTTCCCBTTTTTTT SEQ KESNEEEQVWHFLGK SEG xxxxxxx lhukB Prosite for DKFZphfbr2_64al5.2 PS00387 85->92 PPASE PDOC00325 (No Pfam data available for DKFZphfbr2_64al5.2) Pedant information for DKFZphfbr2_64al5, frame 3 Report for DKFZphfbr2_64al5.3 [LENGTH] 63 [MW] 7405.54 [pi] 6.81 [HOMOL] SWISSPROT :IPYR_DROME INORGANIC PYROPHOSPHATASE (EC 3.6.1.1) (PYROPHOSPHATE PHOSPHO- HYDROLASE) (PPASE) . le-06 [EC] 3.6.1.1 Inorganic pyrophosphatase 5e-06 [PIRKW] hydrolase 5e-06 [SUPFAM] inorganic pyrophosphatase 5e-06 [KW] All_Beta SEQ MALYHTEERGQPCSQNYRLFFKNVTGHYISPFHDIPLKVNSKEDTEAQGIFIDLSKIWKM PRD cccccccccccccccceeeeeecccccccccccccccccccccccccceeeechhhhhhh SEQ AFL PRD CCC (No Prosite data available for DKFZphfbr2_64al5.3) (No Pfam data available for DKFZphfbr2_64al5.3) DKFZphfbr2_64cl6 group: brain derived DKFZphfbr2_64al6.2 encodes a novel 101 amino acid protein without similarity to known proteins . No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of bram-specific genes . unknown complete cDNA, complete eds, EST hits Sequenced by Qiagen Locus: /map="745_A_2; 756_F_2; 842_C_2" Insert length: 1866 bp Poly A stretch at pos. 1848, polyadenylation signal at pos. 1829 1 GGGCGCGGCG CCGGAGGAGG AAGTGGTGAG GTTGTTGCTC CTTCAGCGCC 51 TATCGCTGGC TCTTGGGGCG CAGAGAGGGG CCGCAGTCTC CGCGGCTGCG 101 TCGAGCTCCC TTGCAGTCCC CTCCATGTTC CCCGGCGCCA CTACTCCCCT 151 TCCTAAGGCC GCCGCTTACC CCGGGGTCTA TGGAAGTAAT GGAAGGACCC 201 CTCAACCTGG CTCATCAACA GAGCAGACGA GCAGACCGTT TATTAGCTGC 251 AGGCAAATAC GAAGAGGCTA TTTCTTGTCA CAAAAAGGCT GCAGCATATC 301 TTTCTGAAGC CATGAAGCTG ACACAGTCAG AGCAGGCTCA TCTTTCACTG 351 GAATTGCAAA GGGATAGCCA TATGAAACAG CTCCTCCTCA TCCAAGAGAG 401 ATGGAAAAGG GCCCAGCGTG AAGAAAGATT GAAAGCCCAG CAGAACACAG 451 ACAAGGATGC AGCTGCCCAT CTTCAGACAT CTCACAAACC CTCTGCAGAG 501 GATGCAGAGG GCCAGAGTCC CCTTTCTCAG AAGTACAGCC CTTCCACAGA 551 GAAATGCCTG CCTGAGATTC AGGGGATCTT TGACAGGGAT CCAGACACAC 601 TACTTTATTT ACTTCAGCAA AAGAGTGAGC CAGCAGAGCC ATGTATTGGA 651 AGCAAAGCCC CAAAAGATGA TAAAACAATT ATAGAGGAGC AGGCAACCAA 701 AATTGCAGAT TTGAAGAGGC ATGTGGAATT CCTTGTGGCT GAGAATGAAA 751 GATTAAGGAA AGAAAATAAA CAACTAAAGG CTGAAAAGGC CAGACTTCTA 801 AAAGGTCCAA TAGAAAAGGA GCTGGATGTA GATGCTGATT TTGTAGAAAC 851 GTCAGAGTTA TGGAGCTTGC CACCACATGC AGAAACTGCT ACAGCCTCCT 901 CAACCTGGCA GAAGTTCGCA GCAAATACTG GGAAAGCCAA GGACATTCCA 951 ATCCCCAATC TTCCTCCCTT GGATTTTCCA TCTCCAGAAC TTCCTCTTAT 1001 GGAGCTCTCT GAGGATATTC TGAAAGGACT TATGAATAAT TAAAATGGAA 1051 GGCCACAGAA AAGGGGAAAA GAGGAAATAA TACAGTAATC GTTAATCCAG 1101 CAAAAAGAAA TGAAAAGGGA AAACCACATA GAAGGGTAAT CCCGGAAATG 1151 CTTCATCTGG TGGACTGTGG GAGCAGAGGC ATTGCCAGGA CTTGGGAAAC 1201 AGTCACTGTG AAATGCGCTG CGTATCTCAT TCACTCACTT CAGCTAATGA 1251 CTCCGACTTG GCAGACGCTA AACTCATGGA GGTTCGGTTT CTCCTGATAC 1301 AAACCAAATG GCTACCTGGA AGAATTTCTT TCAAGCAACA GTTATTTTTC 1351 TTATCTTCAG GGTTAAAATG TATAAAAGTT ATGTGTAATT AATCTATAAT 1401 GCCATAAATG ATAATGCAAA ACCTAAATAA TATGGTGGCC GGAGGGGCTG 1451 CCTTATATTT GAAACATGCT TTCTATCATG CATTGACTGT ATGCATTTTG 1501 TTAATGCACA TTCTGTTTGT TTAAGGTGTG TGAGATACAC ACCTTTCTAG 1551 ATGAAACTAT ATGTGCCACA CTTTGCACTA CTCATAATGA TAACCTCAAG 1601 ACTATCAGAA GAAATATTTA AATTTCCATT TTATGAAGAA AGGAACCAAA 1651 TTATTATGCT TTTTAAAACA AATTACCAGT TTACATAATT AATCAGGGTG 1701 CATTTTAAGT TCTAACTTCG TTTATTGTAT AATGCATCAT TTGAAAATAC 1751 CAAGGAGGAA ATACCCTTTG TTTTTAATGA TGCAAGAGTG GACGTAATGC 1801 TAGTTGGCAG TATTTTATTG TAAGAAATCA ATAAAGTAAT TGTGTTTTAA 1851 AAAAAAAAAA AAAAAA BLAST Results Entry HS286143 from database EMBL: human STS WI-6844. Score = 1460, P = 3.4e-61, identities = 292/292 Medline entries No Medline entry Peptide information for. frame 2 ORF from the beginning to 304 bp; peptide length: 102 Category: questionable ORF Classification: unset 1 GAAPEEEVVR LLLLQRLSLA LGAQRGAAVS AAASSSLAVP SMFPGATTPL 51 PKAAAYPGVY GSNGRTPQPG SSTEQTSRPF ISCRQIRRGY FLSQKGCSIS 101 F BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_64cl6, frame 2 No Alert BLASTP hits found Peptide information for frame 3 ORF from 180 bp to 1040 bp; peptide length: 287 Category: putative protein Classification: unset Prosite motifs: LEUCINE_ZIPPER (178-200) LEUCINE ZIPPER (185-207) 1 MEVMEGPLNL AHQQΞRRADR LLAAGKYEEA ISCHKKAAAY LSEAMKLTQS 51 EQAHLSLELQ RDSHMKQLLL IQERWKRAQR EERLKAQQNT DKDAAAHLQT 101 SHKPSAEDAE GQSPLSQKYS PSTEKCLPEI QGIFDRDPDT LLYLLQQKSE 151 PAEPCIGSKA PKDDKTIIEE QATKIADLKR HVEFLVAENE RLRKENKQLK 201 AEKARLLKGP IEKELDVDAD FVETSELWSL PPHAETATAS STWQKFAANT 251 GKAKDIPIPN LPPLDFPSPE LPLMELSEDI LKGLMNN BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_64cl6, frame 3 No Alert BLASTP hits found Pedant information for DKFZphfbr2_64cl6, frame 2 Report for DKFZphfbr2_64cl6.2 [LENGTH] 101 [MW] 10469.94 [pi] 10.18 [KW] All Alpha [KW] LOW COMPLEXITY 29.70 % SEQ GAAPEEEVVRLLLLQRLSLALGAQRGAAVSAAASSSLAVPSMFPGATTPLPKAAAYPGVY SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD ccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccccccccccccccccccccccc SEQ GSNGRTPQPGSSTEQTSRPFISCRQIRRGYFLSQKGCSISF SEG PRD ccccccccccccccccccccchhhhhccccccccccccccc (No Prosite data available for DKFZphfbr2_64cl6.2) (No Pfam data available for DKFZphfbr2_64cl6.2) Pedant information for DKFZphfbr2 64cl6, frame 3 Report for DKFZphfbr2_64cl6.3 [LENGTH] 287 [MW] 32343.79 [pi] 5.61 [PROSITE] LEUCINE ZIPPER 2 [KW] All Alpha [KW] COILED COIL 14.98 % SEQ MEVMEGPLNLAHQQSRRADRLLAAGKYEEAISCHKKAAAYLSEAMKLTQSEQAHLSLELQ PRD ccccchhhhhhhhhhhhhhhhhhhcchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS SEQ RDSHMKQLLLIQERWKRAQREERLKAQQNTDKDAAAHLQTSHKPSAEDAEGQSPLSQKYS PRD hhcchhhhhhhhhhhhhhhhhhhhhhhhccccchhhhhhhcccccccccccccccccccc COILS SEQ PSTEKCLPEIQGIFDRDPDTLLYLLQQKSEPAEPCIGSKAPKDDKTIIEEQATKIADLKR PRD cccccccchhhhhcccccchhhhhhhhhcccccccccccccccchhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCC SEQ HVEFLVAENERLRKENKQLKAEKARLLKGPIEKELDVDADFVETSELWSLPPHAETATAS PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhcccccccccccccccccccccccccccccccc COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCC SEQ STWQKFAANTGKAKDIPIPNLPPLDFPSPELPLMELSEDILKGLMNN PRD hhhhhhhhhcccccccccccccccccccccchhhhhhhhhhhhhccc COILS Prosite for DKFZphfbr2_64cl6.3 PS00029 178->200 LEUCINE_ZIPPER PDOC00029 PS00029 185->207 LEUCINE ZIPPER PDOC00029 (No Pfam data available for DKFZphfbr2_64cl6.3) DKFZphfbr2 64c4 group: brain derived DKFZphfbr2_64c4 encodes a novel 467 ammo acid protein with similarity to A. thaliana T08H3.5 No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of bram-specific genes . similarity to A. thaliana T08I13.5 complete cDNA, complete eds, EST hits on genomic level encoded by AC005043 11 exons Sequenced by Qiagen Locus : unknown Insert length: 1559 bp Poly A stretch at pos. 1540, no polyadenylation signal found 1 TGGGACCGCC GGAAGTTTCT GCCGCGGCTT TGCGGGGACG GGGGAGTGGT 51 AGTGGGGGCT GCAGCTGCCG GACCCAGGCG CGATGGCTAC GGGCGCGGAT 101 GTACGGGACA TTCTAGAACT CGGGGGTCCA GAAGGGGATG CAGCCTCTGG 151 GACCATCAGC AAGAAGGACA TTATCAACCC GGACAAGAAA AAATCCAAGA 201 AGTCCTCTGA GACACTGACT TTCAAGAGGC CCGAGGGCAT GCACCGGGAA 251 GTCTATGCCT TGCTCTACTC TGACAAGAAG GATGCACCCC CACTGCTACC 301 CAGTGACACT GGCCAGGGAT ACCGTACAGT GAAGGCCAAG TTGGGCTCCA 351 AGAAGGTGCG GCCTTGGAAG TGGATGCCAT TCACCAACCC GGCCCGCAAG 401 GACGGAGCAA TGTTCTTCCA CTGGCGACGT GCAGCGGAGG AGGGCAAGGA 451 CTACCCCTTT GCCAGGTTCA A AAGACTGT GCAGGAGCCT GTGTACTCGG 501 AGCAGGAGTA CCAGCTTTAT CTCCACGATA ATGCTTGGAC TAAGGCAGAA 551 ACTGACCACC TCTTTGACCT CAGCCGCCGC TTTGACCTGC GTTTTGTTGT 601 TATCCATGAC CGGTATGACC ACCAGCAGTT CAAGAAGCGT TCTGTGGAAG 651 ACCTGAAGGA GCGGTACTAC CACATCTGTG CTAAGCTTGC CAACGTGCGG 701 GCTGTGCCAG GCACAGACCT TAAGATACCA GTATTTGATG CTGGGCACGA 751 ACGACGGCGG AAGGAACAGC TTGAGCGTCT CTACAACCGG ACCCCAGAGC 801 AGGTGGCAGA GGAGGAGTAC CTGCTACAGG AGCTGCGCAA GATTGAGGCC 851 CGGAAGAAGG AGCGGGAGAA ACGCAGCCAG GACCTGCAGA AGCTGATCAC 901 AGCGGCAGAC ACCACTGCAG AGCAGCGGCG CACGGAACGC AAGGCCCCCA 951 AAAAGAAGCT ACCCCAGAAA AAGGAGGCTG AGAAGCCGGC TGTTCCTGAG 1001 ACTGCAGGCA TCAAGTTTCC AGACTTCAAG TCTGCAGGTG TCACGCTGCG 1051 GAGCCAACGG ATGAAGCTGC CAAGCTCTGT GGGACAGAAG AAGATCAAGG 1101 CCCTGGAACA GATGCTGCTG GAGCTTGGTG TGGAGCTGAG CCCGACACCT 1151 ACGGAGGAGC TGGTGCACAT GTTCAATGAG CTGCGAAGCG ACCTGGTGCT 1201 GCTCTACGAG CTCAAGCAGG CCTGTGCCAA CTGCGAGTAT GAGCTGCAGA 1251 TGCTGCGGCA CCGTCATGAG GCACTGGCCC GGGCTGGTGT GCTAGGGGGC 1301 CCTGCCACAC CAGCATCAGG CCCAGGCCCG GCCTCTGCTG AGCCGGCAGT 1351 GTCTGAACCC GGACTTGGTC CTGACCCCAA GGACACCATC ATTGATGTGG 1401 TGGGCGCACC CCTCACGCCC AATTCGAGAA AGCGACGGGA GTCGGCCTCC 1451 AGCTCATCTT CCGTGAAGAA AGCCAAGAAG CCGTGAGAGG CCCCACGGGG 1501 TGTGGGCGAC GCTGTTATGT AAATAGAGCT GCTGAGTTGG AAAAAAAAAA 1551 AAAAAAAAA BLAST Results Entry AC005043 from database EMBL: Homo sapiens clone NH0576N21; HTGS phase 1, 5 unordered pieces. Score = 1506, P = 4.6e-244, identities = 316/330 Medl e entries No Medline entry Peptide information for frame 2 ORF from 83 bp to 1483 bp; peptide length: 467 Category: similarity to unknown protein 1 MATGADVRDI LELGGPEGDA ASGTISKKDI INPDKKKSKK SSETLTFKRP 51 EGMHREVYAL LYSDKKDAPP LLPSDTGQGY RTVKAKLGΞK KVRPWKWMPF 101 TNPARKDGAM FFHWRRAAEE GKDYPFARFN KTVQEPVYSE QEYQLYLHDN 151 AWTKAETDHL FDLΞRRFDLR FVVIHDRYDH QQFKKRSVED LKERYYHICA 201 KLANVRAVPG TDLKIPVFDA GHERRRKEQL ERLYNRTPEQ VAEEEYLLQE 251 LRKIEARKKE REKRSQDLQK LITAADTTAE QRRTERKAPK KKLPQKKEAE 301 KPAVPETAGI KFPDFKSAGV TLRSQRMKLP SSVGQKKIKA LEQMLLELGV 351 ELSPTPTEEL VHMFNELRSD LVLLYELKQA CANCEYELQM LRHRHEALAR 401 AGVLGGPATP ASGPGPASAE PAVSEPGLGP DPKDTIIDVV GAPLTPNSRK 451 RRESASSSSS VKKAKKP BLASTP hits Entry ATAC2337_5 from database TREMBLNEW: gene: "T08I13.5"; Arabidopsis thaliana chromosome II BAC T08I13 genomic sequence, complete sequence. Score = 340, P = 2.6e-30, identities = 115/374, positives = 176/374 Entry YE8D_SCHPO from database SWISSPROT: HYPOTHETICAL 47.1 KD PROTEIN C9G1.13C IN CHROMOSOME I. Score = 221, P = 1.9e-20, identities = 67/192, positives = 97/192 Entry S64291 from database PIR: hypothetical protein YGR002c - yeast (Saccharomyces cerevisiae) Score = 202, P = 2.8e-13, identities = 71/260, positives = 124/260 Alert BLASTP hits for DKFZphfbr2_64c4, frame 2 No Alert BLASTP hits found Pedant information for DKFZphfbr2_64c4, frame 2 Report for DKFZphfbr2_64c4.2 [LENGTH] 467 [MW] 53007.60 [pi] 9.51 [HOMOL] TREMBL :ATAC2337_5 gene: "T08I13.5"; Arabidopsis thaliana chromosome II BAC T08I13 genomic sequence, complete sequence. 4e-29 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YGR002c] le-19 [PROSITE] MYRISTYL 1 [PROSITE] CAMP_PHOSPHO_SITE 4 [PROSITE] CK2_PHOSPHO_SITE 10 [PROSITE] TYR_PHOSPHO_SITE 3 [PROSITE] GLYCOSAMINOGLYCAN 1 [PROSITE] PKC_PHOSPHO_SITE 12 [PROSITE] ASNJ3-LYCOSYLATION 1 [KW] All_Alpha [KW] LOW_COMPLEXITY 20.13 % SEQ MATGADVRDILELGGPEGDAASGTISKKDIINPDKKKSKKSSETLTFKRPEGMHREVYAL SEG xxxxxxxxxxxxxxxxxx PRD ccceeeeeeeeeeccccccccccccccccccccccccccccccccccccccchhhhhhhh SEQ LYSDKKDAPPLLPSDTGQGYRTVKAKLGSKKVRPWKWMPFTNPARKDGAMFFHWRRAAEE SEG PRD hhhhccccccccccccccccccccccccccccccccccccccccccccchhhhhhhhhhc SEQ GKDYPFARFNKTVQEPVYSEQEYQLYLHDNAWTKAETDHLFDLSRRFDLRFVVIHDRYDH SEG PRD ccccccccccccccccchhhhhhhhhhhcchhhhhhhhhhhhhhhhccceeeeeeccccc SEQ QQFKKRΞVEDLKERYYHICAKLANVRAVPGTDLKIPVFDAGHERRRKEQLERLYNRTPEQ SEG PRD chhhhhhhhhhhhhhhhhhhhhhhhhcccccccccccchhhhhhhhhhhhhhhhhcchhh SEQ VAEEEYLLQELRKIEARKKEREKRSQDLQKLITAADTTAEQRRTERKAPKKKLPQKKEAE SEG xxxxxxxxxxxxxx xxxxxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhchhhhhhhhhhhhhhhhhhhhhhhh SEQ KPAVPETAGIKFPDFKSAGVTLRSQRMKLPSSVGQKKIKALEQMLLELGVELSPTPTEEL SEG xxx PRD hccccccccccccccccceeehhhhhhhccccccchhhhhhhhhhhhhhhhcccccchhh SEQ VHMFNELRSDLVLLYELKQACANCEYELQMLRHRHEALARAGVLGGPATPASGPGPASAE SEG xxxxxxxxxxxxxxxx PRD hhhhhhccchhhhhhhhhhhcccchhhhhhhhhhhhhhhhhccccccccccccccccccc SEQ PAVSEPGLGPDPKDTIIDVVGAPLTPNSRKRREΞASSSSSVKKAKKP SEG xxxxxxx xxxxxxxxxxxxxxxxxxx . PRD cccccccccccccceeeeeccccccccccccccccccccceeecccc Prosite for DKFZphfbr2_64c4.2 PS00001 130->134 ASN_GLYCOSYLATION PDOC00001 PS00002 412->416 GLYCOSAMI OGLYCAN PDOC00002 PS00004 35->39 CAMP_PHOSPHO_SITE PDOC00004 PS00004 39->43 CAMP_PHOSPHO_ΞITE PDOC00004 PS00004 184->188 CAMP_PHOSPHO_SITE PDOC00004 PS00004 451->455 CAMP_PHOSPHO_SITE PDOC00004 PS00005 26->29 PKC_PHOSPHO_SITE PDOC00005 PS00005 38->41 PKC_PHOSPHO_SITE PDOC00005 PS00005 46->49 PKC_PHOSPHO_SITE PDOC00005 PS00005 63->66 PKC_PHOSPHO_SITE PDOC00005 PS00005 82->85 PKC_PHOSPHO_SITE PDOC00005 PS00005 89->92 PKC_PHOSPHO_SITE PDOC00005 PS00005 164->167 PKC_PHOSPHO_SITE PDOC00005 PS00005 284->287 PKC_PHOSPHO_SITE PDOC00005 PS00005 321->324 PKC_PHOSPHO_SITE PDOC00005 PS00005 324->327 PKC_PHOSPHO_SITE PDOC00005 PS00005 448->451 PKC_PHOSPHO_SITE PDOC00005 PS00005 460->463 PKC_PHOSPHO_SITE PDOC00005 PS00006 3->7 CK2_PHOSPHO_SITE PDOC00006 PS00006 26->30 CK2_PHOSPHO_SITE PDOC00006 PΞ00006 132->136 CK2_PHOSPHO_SITE PDOC00006 PS00006 139->143 CK2_PHOSPHO_SITE PDOC00006 PS00006 153->157 CK2_PHOSPHO_SITE PDOC00006 PS00006 187->191 CK2_PHOSPHO_SITE PDOC00006 PS00006 273->277 CK2_PHOSPHO_SITE PDOC00006 PS00006 277->281 CK2_PHOSPHO_SITE PDOC00006 PS00006 355->359 CK2_PHOSPHO_SITE PDOC00006 PS00006 435->439 CK2_PHOSPHO_SITE PDOC00006 PS00007 131->139 TYR_PHOSPHO_SITE PDOC00007 PS00007 227->235 TYR_PHOSPHO_SITE PDOC00007 PS00007 116->125 TYR_PHOSPHO_SITE PDOC00007 PS00008 14->20 MYRISTYL PDOC00008 (No Pfam data available for DKFZphfbr2_64c4.2) DKFZphfbr2_64h6 group: brain derived DKFZphfbr2_64h6 encodes a novel 176 ammo acid protein with similarity to predicted yeast proteins . No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application m studying the expression profile of brain-specific genes . similarity to S. pombe SPBC337.09 and S. cerevisiae YER044c complete cDNA, complete eds accoring to YER044c/SPBC337.09, start at Bp 111, EST hits Sequenced by Qiagen Locus: /map="14" Insert length: 1212 bp Poly A stretch at pos. 1192, polyadenylation signal at pos. 1168 1 GGGCTGGAGC TGTCCTGGGG GAGCTTGTTT GCGGCAGCGG CTGCTGCTGC 51 CACTGCTGTG CTGGGGGCCC GGTCGCCAGG CAAAAAGCCC TCCCACGTTT 101 GAGGGGAGTC ATGAGCCGTT TCCTGAATGT GTTAAGAAGT TGGCTGGTTA 151 TGGTGTCCAT CATAGCCATG GGGAACACGC TGCAGAGCTT CCGAGACCAC 201 ACTTTTCTCT ATGAAAAGCT CTACACTGGC AAGCCAAACC TTGTGAATGG 251 CCTCCAAGCT CGGACCTTTG GGATCTGGAC GCTGCTCTCA TCAGTGATCC 301 GCTGCCTCTG TGCCATTGAC ATTCACAACA AGACGCTCTA TCACATCACA 351 CTCTGGACCT TCCTCCTTGC CCTGGGGCAT TTCCTCTCTG AGTTGTTTGT 401 CTATGGAACT GCAGCTCCCA CGATTGGCGT CCTGGCACCC CTGATGGTGG 451 CAAGTTTCTC CATCCTGGGT ATGCTGGTCG GGCTCCGGTA TCTAGAAGTA 501 GAACCAGTAT CCAGACAGAA GAAGAGAAAC TGAGGCCAGC ATTATCACCT 551 CCAGGACTTT CTCGTTTTCC ACCTTGGCCA TCTTCTTCCT TCGTCGTCTC 601 TCCCCTTTAA TTTCTTTTCT ATTCCATCAT CTGCCCTTTT ACTCACTTTT 651 AGCCTCTTTT TTTAATTTTT AAAATTTAAA GATATGCATA CTGAAAAGTA 701 TATAACATGT ACGTACAATT TAAAGAATAA TTTTAAAGTG AATACTACGT 751 AACTCCATCC AAGTCAAGAA ATTGCCAGCT TCTCGGAAGC CCACTGTGTC 801 TCCTTCCCCT ACCTGCAACC TCTTCCAGGC TCCCTTTTCC AGCCTTCCCC 851 TTTTTCCCTT TTATTTTCAT GCCTTGATTT GACTTGTGTG GTGGGAACAT 901 GTGAACTATG AAACTTAAAC CTGCTGCCCA CCCAGAGCAG CTGTGACCAA 951 GGGCTGCCTC AAGGGGTTGT CCACGCAGGT TGGGCTCCTC TCTGCTGCTG 1001 GACCCAAGAC TCTGAACCTT CCAAGGGACA GGCAGTTCTT CTGAGAAGGG 1051 CTCCCCTGTG TGTGAGCAAG ACCACAGCTC TCCTTCTATC TACAGATGCA 1101 TGAGGGTTGG AAGAGTCTGG GCTGTTTTTA GACCTTCTGG TCAGCTGTAT 1151 TTGTGTAACA ACTTTTGTAA TAAATAGAAA AACCCTCTGC TCAAAAAAAA 1201 AAAAAAAAAA AA BLAST Results Entry G38566 from database EMBL: SHGC-64295 Human Homo sapiens STS genomic, sequence tagged site. Score = 1398, P = 1.4e-56, identities = 284/288 Medline entries No Medlme entry Peptide information for frame 3 ORF from 0 bp to 530 bp; peptide length: 177 Category: similarity to unknown protein Classification: unclassified 1 AGAVLGELVC GSGCCCHCCA GGPVARQKAL PRLRGVMSRF LNVLRSWLVM 51 VSIIAMGNTL QSFRDHTFLY EKLYTGKPNL VNGLQARTFG IWTLLSSVIR 101 CLCAIDIHNK TLYHITLWTF LLALGHFLSE LFVYGTAAPT IGVLAPLMVA 151 SFSILGMLVG LRYLEVEPVS RQKKRN BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_64h6, frame 3 TREMBL :SPBC337_9 gene: "SPBC337.09"; product: "conserved hypothetical protein"; S. pombe chromosome II cosmid c337., N = 1, Score = 224, P = 1.4e-18 PIR:S50547 hypothetical protein YER044c - yeast (Saccharomyces cerevisiae), N = 1, Score = 192, P = 3.4e-15 >TREMBL:SPBC337_9 gene: "SPBC337.09"; product: "conserved hypothetical protein"; S.pombe chromosome II cosmid c337. Length = 136 HSPs: Score = 224 (33.6 bits), Expect = 1.4e-18, P = 1.4e-18 Identities = 49/113 (43%), Positives = 74/113 (65%) Query: 42 NVLRSWLVMVSIIAMGNTLQSFRDHTFLYEKLYTGKPNLVNGLQARTFGIWTLLSΞVIRC 101 +++ W V+VS+ A+ NT+QSF L +++Y+ N VNGLQ RTFGIWTLLS+++R Sbjct: 11 SLVAKWNVVVSVAALFNTVQSFLTPK-LTKRVYSNT-NEVNGLQGRTFGIWTLLSAIVRF 68 Query: 102 LCAIDIHNKTLYHITLWTFLLALGHFLSELFVYGTAAPTIGVLAPLMVASFSI 154 CA I N +Y + T+ LA HFLSE ++ T G+L+P++V++ SI Sbjct: 69 YCAYHITNPDVYFLCQCTYYLACFHFLSEWLLFRTTNLGPGLLSPIVVSTVSI 121 Pedant information for DKFZphfbr2_64h6, frame 3 Report for DKFZphfbr2_64h6.3 [LENGTH] 176 [MW] 19359.31 [pi] 9.53 [HOMOL] TREMBL :SPBC337_9 gene: "SPBC337.09"; product: "conserved hypothetical protein" S. pombe chromosome II cosmid c337. 2e-17 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YER044c] 7e-16 [KW] TRANSMEMBRANE 2 [KW] LOW COMPLEXITY 7.39 ? SEQ AGAVLGELVCGSGCCCHCCAGGPVARQKALPRLRGVMSRFLNVLRSWLVMVSI IAMGNTL SEG xxxxxxxxxxxxx PRD ccceeeeeeeeccceeeeccccccccccccccccchhhhhhhhhhhhhhheeeecccccc MEM MMMMMMMMMMMMMMMMM .... SEQ QSFRDHTFLYEKLYTGKPNLVNGLQARTFGIWTLLSSVIRCLCAIDIHNKTLYHITLWTF SEG PRD ccccchhhhhhhhhhcccccccccccccccchhhhhhhhhhhhhhhccccceeeehhhhh MEM SEQ LLALGHFLSELFVYGTAAPTIGVLAPLMVASFSILGMLVGLRYLEVEPVSRQKKRN SEG PRD hhhhhhhhhhhhhhhccccccccccceeehhhhhhhhhhhheeeeecccccccccc MEM MMMMMMMMMMMMMMMMM (No Prosite data available for DKFZphfbr2_64h6.3) (No Pfam data available for DKFZphfbr2_64h6.3) DKFZphfbr2_64jl8 group: Intracellular transport and trafficking DKFZphfbr2_624jl8.1 encodes a novel 180 amino acid protein nearly identical to the microsomal signal peptidase 23 kd subunit of cams familiaris, gallus gallus and C. elegans. The new protein is identical to canine and chicken microsomal signal peptidase 23 kd subunit. The canine microsomal signal peptidase is a protein complex comprised of five subunits (25, 22/23, 21, 18, and 12 kDa) . The 23kDa subunit is tightly associated with the 18- and 21-kDa subunits, that are integral membrane proteins. The new protein can find application in modulation of protein transport into microsomal compartments and as a tool for proteomic analysis. strong similarity to dog signal peptidase (EC 3.4.99.-) complete cDNA, complete eds, potential start at Bp 109, EST hits, Sequenced by Qiagen Locus : unknown Insert length: 690 bp Poly A stretch at pos. 666, polyadenylation signal at pos. 646 1 GCCGGAACGC GCGCACCGCA GACGGCGCGG ATCGCAGGGA GCCGGTCCGC 51 CGCCGGAACG GGAGCCTGGG TGTGCGTGTG GAGTCCGGAC TCGTGGGAGA 101 CGATCGCGAT GAACACGGTG CTGTCGCGGG CGAACTCACT GTTCGCCTTC 151 TCGCTGAGCG TGATGGCGGC GCTCACCTTC GGCTGCTTCA TCACCACCGC 201 CTTCAAAGAC AGGAGCGTCC CGGTGCGGCT GCACGTCTCG CGGATCATGC 251 TAAAAAATGT AGAAGATTTC ACTGGACCTA GAGAAAGAAG TGATCTGGGA 301 TTTATCACAT CTGATATAAC TGCTGATCTA GAGAATATAT TTGATTGGAA 351 TGTTAAGCAG TTGTTTCTTT ATTTATCAGC AGAATATTCA ACAAAAAATA 401 ATGCTCTGAA CCAAGTTGTC CTATGGGACA AGATTGTTTT GAGAGGTGAT 451 AATCCGAAGC TGCTGCTGAA AGATATGAAA ACAAAATATT TTTTCTTTGA 501 CGATGGAAAT GGTCTCAAGG GAAACAGGAA TGTCACTTTG ACCCTGTCTT 551 GGAACGTCGT ACCAAATGCT GGAATTCTAC CTCTTGTGAC AGGATCAGGA 601 CACGTATCTG TCCCATTTCC AGATACATAT GAAATAACGA AGAGTTATTA 651 AATTATTCTG AATTTGAAAC AAAAAAAAAA AAAAAAAAAA BLAST Results No BLAST result Medline entries 89034208: cDNA-deπved primary structure of the glycoprotein component of canine microsomal signal peptidase complex. Peptide information for frame 1 ORF from 109 bp to 648 bp; peptide length: 180 Category: strong similarity to known protein Prosite motifs: TONB_DEPENDENT_REC_l (1-58) RGD (148-151) 1 MNTVLSRANS LFAFSLSVMA ALTFGCFITT AFKDRSVPVR LHVSRIMLKN 51 VEDFTGPRER SDLGFITSDI TADLENIFDW NVKQLFLYLS AEYSTKNNAL 101 NQVVLWDKIV LRGDNPKLLL KDMKTKYFFF DDGNGLKGNR NVTLTLSWNV 151 VPNAGILPLV TGSGHVSVPF PDTYEITKSY BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_64j 18, frame 1 No Alert BLASTP hits found Pedant information for DKFZphfbr2_64j 18, frame 1 Report for DKFZphfbr2_64j 18.1 [LENGTH] 180 [MW] 20253.39 [pi] 8.66 [HOMOL] PIR:A31788 signal peptidase (EC 3.4.99.-) (SPC 22/23) - dog le-100 [FUNCAT] 30.07 organization of endoplasmatic reticulum [S. cerevisiae, YLR066w] 6e-15 [FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylation, farnesylation and processing) [S. cerevisiae, YLR066w] 6e-15 [PIRKW] transmembrane protein 2e-92 [PIRKW] glycoprotein 2e-92 [PIRKW] hydrolase 2e-92 [PROSITE] RGD 1 [PROSITE] MYRISTYL 2 [PROSITE] PROKAR_LIPOPROTEIN 1 [PROSITE] TONB_DEPENDENT_REC_l 1 [PROSITE] PKC_PHOSPHO_SITE 1 [PROSITE] ASN_GLYCOSYLATION 1 [KW] Alpha_Beta [KW] SIGNAL PEPTIDE 32 SEQ. MNTVLSRANSLFAFSLSVMAALTFGCFITTAFKDRSVPVRLHVSRIMLKNVEDFTGPRER PRD ccccccchhhhhhhhhhhhhhhhhhhhhheeecccccceeehhhhhhhhhhhhccccccc SEQ SDLGFITSDITADLENIFDWNVKQLFLYLSAEYSTKNNALNQVVLWDKIVLRGDNPKLLL PRD ccccchhhhhhhhccccccchhhhhhhhhhhhhhhccccceeeeeeeceeecccchhhhh SEQ KDMKTKYFFFDDGNGLKGNRNVTLTLSWNVVPNAGILPLVTGSGHVSVPFPDTYEITKSY PRD hhcccceeeeecccccccccceeeeeeeecccccceeeeeccccceeeeccccccccccc Prosite for DKFZphfbr2_64 j 18.1 PS00001 141->145 ASN_GLYCOSYLATION PDOC00001 PS00005 94->97 PKC_PHOSPHO_SITE PDOC00005 PS00008 25->31 MYRISTYL PDOC00008 PS00008 135->141 MYRISTYL PDOC00008 PS00013 16->27 PROKAR_LIPOPROTEIN PDOC00013 PS00016 112-M15 RGD PDOC00016 PS00430 l->22 TONB DEPENDENT REC 1 PDOC00354 (No Pfam data available for DKFZphfbr2_64 l8.1) DKFZphfbr2_64k24 group: transmembrane proteins DKFZphfbr2_64k24 encodes a novel 412 am o acid protein with weak similarity to several known proteins . The novel protein contains 5 transmembrane regions. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of brain-specific genes and as a new marker for neuronal cells. similarity to AMAC1 "testicular condensing enzyme" ; membrane regions: 5 Summary DKFZphfbr2_64k24 encodes a novel 412 ammo acid protein, with similarity to AMAC1"; product: "testicular condensing enzyme similarity to AMAC1 "testicular condensing enzyme" complete cDNA, complete eds, EST hits Sequenced by Qiagen Locus : unknown Insert length: 1958 bp Poly A stretch at pos. 1939, polyadenylation signal at pos. 1918 1 GGGCCCGCCT CGATTTTCCC AGGCGAGGGC ACGCCCGCGT CAGTCGCCTC 51 CGGGGCACCT TCCTCGCCAC GACACGCAGG TAACCGGGCC CCGGGAGCCG 101 GTCGGCGGCG GCGGACTGGG ACCTTGATCC TGCCTGCCCG GCCGCCCGAC 151 AAGGGAATGA GAGCGGACCC CGAACTCCAC ACACCCGCGT TTAGCCGCCA 201 CACCTAAGGG GCAGAACAGT CTTTTTGGGT AAGGGCCGGG CTGGGGGCGA 251 CGCGCCCCGC CCGCTTTGCA GACTTCGGGG TGCTCTGCAC GACGCCTGAA 301 AGGCCGCGGG GCCCGCATTT CTCTGTGCTG CCCTCCTGGA GAACCGGGAC 351 ACGGGGACGG GAGGGCCAGC ATCGGCTACG GCCCGGTTTC CCGTTTCTTT 401 CCTCTGTCGC GTCTGGGCCC TCCTGCAGCG TCCATGATGA AGGCCAGGGG 451 CTGTTGCTTT CCTCTCGCCC AGTAGCCAAC CCAAGCAAGG GAATTAATTA 501 TCTGAAGAAA TGGATACTTC TCCCTCCAGA AAATATCCAG TTAAAAAACG 551 GGTGAAAATA CATCCCAACA CAGTGATGGT GAAATATACT TCTCATTATC 601 CCCAGCCTGG CGATGATGGA TATGAAGAAA TCAATGAAGG CTATGGGAAT 651 TTTATGGAGG AAAATCCAAA GAAAGGTCTG CTGAGTGAAA TGAAAAAAAA 701 AGGGAGAGCT TTCTTTGGAA CCATGGATAC CCTACCTCCA CCAACAGAAG 751 ACCCAATGAT CAATGAGATT GGACAATTCC AGAGCTTTGC AGAAAAAAAC 801 ATTTTTCAAT CCCGAAAAAT GTGGATAGTG CTGTTTGGAT CTGCTTTGGC 851 TCATGGATGT GTAGCTCTTA TCACTAGGCT TGTTTCTGAT CGGTCTAAAG 901 TTCCATCTCT AGAACTGATT TTTATCCGTT CTGTTTTTCA GGTCTTATCT 951 GTGTTAGTTG TGTGTTACTA TCAGGAGGCC CCCTTTGGAC CCAGTGGATA 1001 CAGATTACGA CTCTTCTTTT ATGGTGTATG CAATGTCATT TCTATCACTT 1051 GTGCTTATAC ATCATTTTCA ATAGTTCCTC CCAGCAATGG GACCACTATG 1101 TGGAGAGCCA CAACTACAGT CTTCAGTGCC ATTTTGGCTT TTTTACTCGT 1151 AGATGAGAAA ATGGCTTATG TTGACATGGC TACAGTTGTT TGCAGCATCT 1201 TAGGTGTTTG TCTTGTCATG ATCCCAAACA TTGTTGATGA AGACAATTCT 1251 TTGTTAAATG CCTGGAAAGA AGCCTTTGGG TACACCATGA CTGTGATGGC 1301 TGGACTGACC ACTGCTCTCT CAATGATAGT ATACAGATCC ATCAAGGAGA 1351 AGATCAGCAT GTGGACTGCG CTGTTTACTT TTGGTTGGAC TGGGACAATT 1401 TGGGGAATAT CTACTATGTT TATTCTTCAA GAACCCATCA TCCCATTAGA 1451 TGGAGAAACC TGGAGTTATC TCATTGCTAT ATGTGTCTGT TCTACTGCAG 1501 CATTCTTAGG AGTTTATTAT GCCTTGGACA AATTCCATCC AGCTTTGGTT 1551 AGCACAGTAC AACATTTGGA GATTGTGGTA GCTATGGTCT TGCAGCTTCT 1601 CGTGCTGCAC ATATTTCCTA GCATCTATGA TGTTTTTGGA GGGGTAATCA 1651 TTATGATTAG TGTTTTTGTC CTTGCTGGCT ATAAACTTTA CTGGAGGAAT 1701 TTAAGAAGGC AGGACTACCA GGAAATACTA GACTCTCCCA TTAAATGAAT 1751 ACCTGATTAT TATTGTCTCA TTAATGTTCA GTTATTAATA TGTATACTGC 1801 CATTTTAATG TTTACCTATG AATGTCTTTT GTGTTATATA ACTGACAGAG 1851 TGCTATAAAA TATATAATAT ATACAAATGC AGAAAATTTA TTCTAGTCTA 1901 ATATATTCAA ATACAAATAT TAAATATATG AAATACGTTA AAAAAAAAAA 1951 AAAAAAAA BLAST Results o BLAST result Medline entries No Medline entry Peptide information for frame 3 ORF from 510 bp to 1745 bp; peptide length: 412 Category: similarity to known protein 1 MDTSPSRKYP VKKRVKIHPN TVMVKYTSHY PQPGDDGYEE INEGYGNFME 51 ENPKKGLLSE MKKKGRAFFG TMDTLPPPTE DPMINEIGQF QSFAEKNIFQ 101 SRKMWIVLFG SALAHGCVAL ITRLVSDRSK VPSLELIFIR SVFQVLSVLV 151 VCYYQEAPFG PSGYRLRLFF YGVCNVISIT CAYTSFΞIVP PSNGTTMWRA 201 TTTVFSAILA FLLVDEKMAY VDMATVVCSI LGVCLVMIPN IVDEDNSLLN 251 AWKEAFGYTM TVMAGLTTAL SMIVYRSIKE KISMWTALFT FGWTGTIWGI 301 STMFILQEPI IPLDGETWSY LIAICVCSTA AFLGVYYALD KFHPALVSTV 351 QHLEIVVAMV LQLLVLHIFP SIYDVFGGVI IMISVFVLAG YKLYWRNLRR 401 QDYQEILDSP IK BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_64k24, frame 3 TREMBLNEW:AF016712_1 gene: "AMACl"; product: "testicular condensing enzyme"; Mus musculus testicular condensing enzyme (AMACl) mRNA, complete eds., N = 1, Score = 191, P = 1.9e-12 TREMBL :BMAJ733_6 product: "hypothetical protein"; Bacillus megaterium bgaM gene, N = 1, Score = 137, P = 1.6e-06 PIR:G71841 hypothetical protein jhpll55 - Helicobacter pylori (strain J99), N = 1, Score = 129, P = 1.3e-05 >TREMBLNEW:AF016712_1 gene: "AMACl"; product: "testicular condensing enzyme"; Mus musculus testicular condensing enzyme (AMACl) mRNA, complete eds . Length = 362 HSPs: Score = 191 (28.7 bits), Expect = 1.9e-12, P = 1.9e-12 Identities = 39/105 (37%), Positives = 66/105 (62%) Query: 289 FTFGWTGTIWGISTMFILQEPIIPLDGETWSYLIAICVCSTAAFLGVYYALDKFHPALVS 348 F FG G + + +F+LQ P++P D +WS ++A+ + + +F+ V YA+ K HPALV Sbjct: 248 FLFGLVGLMVSVPGLFVLQTPVLPQDTLSWSCVVAVGLLALVSFVCVSYAVTKAHPALVC 307 Query: 349 TVQHLEIVVAMVLQLLVLH—IFPSIYDVFGGVIIMISVFVLAGYKL 393 V H E+VVA++LQ VL+ + PS D+ G +++ Ξ+ ++ L Sbjct: 308 AVLHSEVVVALMLQYYVLYETVAPS--DIMGAGVVLGSIAIITAQNL 352 Pedant information for DKFZphfbr2_64k24, frame 3 Report for DKFZphfbr2_64k24.3 [LENGTH] 412 [MW] 46449.87 [pi] 6.99 [HOMOL] TREMBL:AF016712_1 gene: "AMACl"; product: "testicular condensing enzyme"; Mus musculus testicular condensing enzyme (AMACl) mRNA, complete eds. 8e-14 [PROSITE] MYRISTYL 6 [PROSITE] CK2_PHOSPHO_SITE 3 [PROSITE] PKC_PHOSPHO_SITE 4 [PROSITE] ASN_GLYCOSYLATION 1 [KW] TRANSMEMBRANE 5 SEQ MDTSPSRKYPVKKRVKIHPNTVMVKYTSHYPQPGDDGYEEINEGYGNFMEENPKKGLLSE PRD ccccccccccccceeeecccceeeeeecccccccccceeeeecccccccccccccchhhh MEM SEQ MKKKGRAFFGTMDTLPPPTEDPMINEIGQFQSFAEKNIFQSRKMWIVLFGSALAHGCVAL PRD hhhhcceeecccccccccccccceeeecccchhhhhhhhccceeeeeeeccccchhhhhc MEM SEQ ITRLVSDRSKVPSLELIFIRSVFQVLSVLVVCYYQEAPFGPSGYRLRLFFYGVCNVISIT PRD chhhhhccccccccchhhhhhhhhhhheeeeeeeccccccccceeeeeeeecceeeeeee MEM MMMMMMMMMMMMMMMMM SEQ CAYTSFSIVPPSNGTTMWRATTTVFΞAILAFLLVDEKMAYVDMATVVCSILGVCLVMIPN PRD eccceeeeccccccceeeeeehhhhhhhhhhhhhhhhheeeeeeeeeeeeeeeeeeeecc MEM SEQ IVDEDNSLLNAWKEAFGYTMTVMAGLTTALSMIVYRSIKEKISMWTALFTFGWTGTIWGI PRD cccccchhhhhhhhhhhheeeeeeehhhhhhhcchhhhhhhhhhhhccccccccceeeec MEM MMMMMMMMMMMMMMMMMMM SEQ STMFILQEPIIPLDGETWSYLIAICVCSTAAFLGVYYALDKFHPALVSTVQHLEIVVAMV PRD ceeeeeecccccccccceeeeeccchhhhhhhhhccccccccccchhhhhhhhhhhhhhh MEM MMMMMMMMMM MMMMMMMMMMMMMMMMMMMMMMM MMMMMMMMMMMMMM SEQ LQLLVLHIFPSIYDVFGGVIIMISVFVLAGYKLYWRNLRRQDYQEILDSPIK PRD hhhhhhhhhccccccceeeeeeeeeecccccchhhhhhhhhhhhhhhccccc MEM MMMMMMM....MMMMMMMMMMMMMMMMMMMMM Prosite for DKFZphfbr2_64k24.3 PS00001 193->197 ASN_GLYCOSYLATION PDOC00001 PS00005 6->9 PKC_PHOSPHO_SITE PDOC00005 PS00005 101->104 PKC_PHOSPHO_SITE PDOC00005 PS00005 126->129 PKC_PHOSPHO_SITE PDOC00005 PS00005 277->280 PKC_PHOSPHO_ΞITE PDOC00005 PS00006 92->96 CK2_PHOSPHO_SITE PDOC00006 PS00006 277->281 CK2_PHOSPHO_SITE PDOC00006 PS00006 371->375 CK2_PHOSPHO_SITE PDOC00006 PS00008 70->76 MYRISTYL PDOC00008 PS00008 88->94 MYRISTYL PDOC00008 PS00008 110->116 MYRISTYL PDOC00008 PS00008 265->271 MYRISTYL PDOC00008 PS00008 295->301 MYRISTYL PDOC00008 PS00008 334->340 MYRISTYL PDOC00008 (No Pfam data available for DKFZphfbr2_64k24.3) DKFZphfbr2 6al7 group: brain derived DKFZphfbr2_6al7 encodes a novel 100 amino acid protein with very weak similarity to human finger protein zfOCl. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of brain-specific genes . complete cDNA, complete eds, EST hits Sequenced by AGOWA Locus : unknown Insert length: 1424 bp Poly A stretch at pos. 1405, polyadenylation signal at pos. 1389 1 GGGACTGAGG GGGTGGGCTT ACTCCCTGGG CAGTCTTGGG GGCCAGAGCT 51 GAGGCCAGTC CATATTACAG TGGCTGGGCT GTTTTTTTCA GTAGCCCCTA 101 GCATTGGCTG GGATTCCTGT TCCTGGGTGC GCCTCCACCT CCCTTCTGAT 151 GCTTCCTGGC TATGGTGGGG TGGGAACCTC AGTTTCCCCC AAAGTCTTCC 201 CTGGATGCTG GCTTCAGGTT GAAGACCCTG GTTCTTCCAG TTCCTCACGG 251 GTTAGGTAGG GGCTCCTGCA TCACCTTCAG AATCAGTTCC AACCCCCACT 301 CTCCTTAGGC TTTGTGCTCT GCTCTGCCCT GCCAGGCTGC CCTTGTCCAT 351 GTGAGTAGCA TGGGCGGGTG GTGGGGACGG CAGTGGTGAT GAAGGGGGTG 401 CACCACAGGC CTCATGAAGC AGTTCCCACA TGGGCGTGTG GCTGGGGCGT 451 GGCCACCACA GAGCACATGG CTGTGTCTAG GCGCAAGCAC TTTAGCAGTA 501 TCTGTTTACA TGCGCAAGGA TCAAGCCGAC TACCTGTGCT GTCTACTGGG 551 ACAGCAGTCT CCGAGCTACT CCGTACCTCC CTCTGCCAGG TCGTGGAGTT 601 AGGCCCCAGT CCCTACTTGT CACTGGTTCC CACTGTGCTC CTAACTGTGC 651 AGCACCTGGG AGCTCTGGCC TGGGGCTGGA GGCCCTGGTA GGAGCTGCAG 701 TTGGAGGCCG TTCTGTGCCC AGCAGCGGTG AGCGGCTCCC ATGGGCCCTG 751 TGTCTGCAGG GAGCCAGGGC TGCGGCACAT GTGCTGTGAA ACTGGCACCC 801 ACCTGGCGTG CTGCTGCCGC CACTTGCTTC CTGCAGCACC TCCTACCCTG 851 CTCCGTGTCC TCCCTCTCCC CGCGCCTGGC TCAGGAGTGC TGGAAAAGCT 901 CACGCCTCGG CCTGGGAGCC TGGCCTCTTG ATATACCTCG AGCTTCCCCT 951 GTGCTCCCCA GCCCCAGGAC CACTGGCCCC TTGGCCTGAG GGGCTGGGGG 1001 CCCCACGACC TGCAGCGTCG AGTCCGGGAG AGAGCCCGGA GCGGCGTGCC 1051 ATCTCGGCTC GGCCTTGCTG AGAGCCTCCG CCCTGGCTTT CTCCCTGTCT 1101 GGTTTCAGTG GCTCACGTTG GTGCTACACA GCTAGAATAG ATATATTTAG 1151 AGAGAGAGAT ATTTTTAAGA CAAAGCCCAC AATTAGCTGT CCTTTAACAC 1201 CGCAGAACCC CCTCCCAGAA GAAGAGCGAT CCCTCGGACG GTCCGGGCGG 1251 GCACCCTCAG CCGGGCTCTT TGCAGAAGCA GCACCGCTGA CTGTGGGCCC 1301 GGCCCTCAGA TGTGTACATA TACGGCTATT TCCTATTTTA CTGTTCTTCA 1351 GATTTAGTAC TTGTAAATAA ACACACACAT TAAGGAGAGA TTAAACATTT 1401 TTGCCAAAAA AAAAAAAAAA AAAA BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 2 ORF from 389 bp to 688 bp; peptide length: 100 Category: putative protein 1 MKGVHHRPHE AVPTWACGWG VATTEHMAVS RRKHFSSICL HAQGΞSRLPV 51 LSTGTAVSEL LRTSLCQVVE LGPSPYLSLV PTVLLTVQHL GALAWGWRPW BLASTP hits Entry S70007 from database PIR : fmger protein zfOCl - human ( fragment ) Length = 183 Score = 62 (21.8 bits), Expect = 0.24, Sum P(2) = 0.22 Identities = 18/47 (38%), Positives = 24/47 (51%) Alert BLASTP hits for DKFZphfbr2_6al7, frame 2 No Alert BLASTP hits found Pedant information for DKFZphfbr2_6al7, frame 2 Report for DKFZphfbr2_6al7.2 [LENGTH] 100 [MW] 10944.82 [pi] 9.49 [PROSITE] MYRISTYL 2 [PROSITE] PKC_PHOSPHO_SITE [KW] Alpha Beta SEQ MKGVHHRPHEAVPTWACGWGVATTEHMAVSRRKHFSSICLHAQGSSRLPVLSTGTAVSEL PRD cccccccccccccccccccccchhhhhhhhhhcccccceeeccccccceeecccchhhhh SEQ LRTΞLCQVVELGPSPYLSLVPTVLLTVQHLGALAWGWRPW PRD hhhhheeeeecccccceeecchhhhhhhhhchhhhhcccc Prosite for DKFZphfbr2_6al7.2 PS00005 30->33 PKC_PHOSPHO_SITE PDOC00005 PS00005 45->48 PKC_PHOSPHO_SITE PDOC00005 PS00008 20->26 MYRISTYL PDOC00008 PS00008 54->60 MYRISTYL PDOC00008 (No Pfam data available for DKFZphfbr2_6al7.2) DKFZphfbr2_6b24 group: metabolism DKFZphfkd2 6b24 encodes a novel 334 amino acid protein with similarity to several bacterial dTDP-4-dehydrorhamnose reductases (EC 1.1.1.133). The novel protein seems to be a human enzyme similar to dTDP-4-dehydrorhamnose reductases. EC 1.1.1.133 catalises the reaction: dTDP-6-deoxy-L-mannose + NADP(+) <=> dTDP-4-dehydro-6-deoxy- L-mannose + NADPH. The new protein can find application in modulation of rhamnose metabolism and as a new enzyme for biotechnologic production processes. similar to dTDP-6-deoxy-L-mannose-dehydrogenases complete cDNA, EST hits, complete eds Nucleotide sugars metabolism seems to be a dehydrogenase localisation: region of primer A missing Sequenced by AGOWA Locus: /map="5" Insert length: 2054 bp Poly A stretch at pos. 2028, polyadenylation signal at pos. 2015 1 GGGGGAGGCC CGCGTCGATC CTGGGTTGGA GGAGGTGGCG GCCGCTGAGG 51 CTGCGGCGTG AAGACGGCGG GCATGGTGGG GCGGGAGAAA GAGCTCTCTA 101 TACACTTTGT TCCCGGGAGC TGTCGGCTGG TGGAGGAGGA AGTTAACATC 151 CCTAATAGGA GGGTTCTGGT TACTGGTGCC ACTGGGCTTC TTGGCAGAGC 201 TGTACACAAA GAATTTCAGC AGAATAATTG GCATGCAGTT GGCTGTGGTT 251 TCAGAAGAGC AAGACCAAAA TTTGAACAGG TTAATCTGTT GGATTCTAAT 301 GCAGTTCATC ACATCATTCA TGATTTTCAG CCCCATGTTA TAGTACATTG 351 TGCAGCAGAG AGAAGACCAG ATGTTGTAGA AAATCAGCCA GATGCTGCCT 401 CTCAACTTAA TGTGGATGCT TCTGGGAATT TAGCAAAGGA AGCAGCTGCT 451 GTTGGAGCAT TTCTCATCTA CATTAGCTCA GATTATGTAT TTGATGGAAC 501 AAATCCACCT TACAGAGAGG AAGACATACC AGCTCCCCTA AATTTGTATG 551 GCAAAACAAA ATTAGATGGA GAAAAGGCTG TCCTGGAGAA CAATCTAGGA 601 GCTGCTGTTT TGAGGATTCC TATTCTGTAT GGGGAAGTTG AAAAGCTCGA 651 AGAAAGTGCA GTGACTGTTA TGTTTGATAA AGTGCAGTTC AGCAACAAGT 701 CAGCAAACAT GGATCACTGG CAGCAGAGGT TCCCCACACA TGTCAAAGAT 751 GTGGCCACTG TGTGCCGGCA GCTAGCAGAG AAGAGAATGC TGGATCCATC 801 AATTAAGGGA ACCTTTCACT GGTCTGGCAA TGAACAGATG ACTAAGTATG 851 AAATGGCATG TGCAATTGCA GATGCCTTCA ACCTCCCCAG CAGTCACTTA 901 AGACCTATTA CTGACAGCCC TGTCCTAGGA GCACAACGTC CGAGAAATGC 951 TCAGCTTGAC TGCTCCAAAT TGGAGACCTT GGGCATTGGC CAACGAACAC 1001 CATTTCGAAT TGGAATCAAA GAATCACTTT GGCCTTTCCT CATTGACAAG 1051 AGATGGAGAC AAACGGTCTT TCATTAGTTT ATTTGTGTTG GGTTCTTTTT 1101 TTTTTTAAAT GAAAAGTATA GTATGTGGCC CTTTTTAAAG AACAAAGGAA 1151 ATAGTTTTGT ATGAGTACTT TAATTGTGAC TCTTAGGATC TTTCAGGTAA 1201 ATGATGCTCT TGCACTAGTG AAATTGTCTA AAGAAACTAA AGGGCAGTCA 1251 TGCCCTGTTT GCAGTAATTT TTCTTTTTAT CATTATGTTT GTCCTGGCTA 1301 AACTTGGAGT TTGAGTATAG TAAATTATGA TCCTTAAATA TTTGAGGGTC 1351 AGGATGAAGC AGATCTGCTG TAGACTTTTC AGATGAAATT GTTCATTCTC 1401 GTAACCTCCA TATTTTCAGG ATTTTTGAAG CTGTTGACCA TTTCATGTTG 1451 ATTATTTTAA ATTGTGTGGA ATAGTATAAA AATCATTGGT GTTCATTATT 1501 TGCTTTGCCT GAGCTCAGAT CAAAATGTTT GAAGAAAGGA ACTTTATTTT 1551 TGCAAGTTAC GTACAGTTTT TATGCTTGAG ATATTTCAAC ATGTTATGTA 1601 TATTGGAACT TCTACAGCTT GATGCCTCCT GCTTTTATAG CAGTTTATGG 1651 GGAGCACTTG AAAGAGCGTG TGTACATGTA TTTTTTTTCT AGGCAAACAT 1701 TGAATGCAAA CGTGTATTTT TTTAATATAA ATATATAACT GTCCTTTTCA 1751 TCCCATGTTG CCGCTAAGTG ATATTTCATA TGTGTGGTTA TACTCATAAT 1801 AATGGGCCTT GTAAGTCTTT TCACCATTCA TGAATAATAA TAAATATGTA 1851 CTGCTGGCAT GTAATGCTTA GTTTTCTTGT ATTTACTTCT TTTTTTTAAA 1901 TGTAAGGACC AAACTTCTAA ACTAATTGTT CTTTTGTTGC TTTAATTTTT 1951 AAAAATTACA TTCTTCTGAT GTAACATGTG ATACATACAA AAGAATATAG 2001 TTTAATATGT ATTGAAATAA AACACAATAA AATTAAAAAA AAAAAAAAAA 2051 AAAA BLAST Results Entry G37115 from database EMBL: SHGC-56899 Human Homo sapiens STS genomic. Score = 446, P = 4.6e-14, identities = 90/91 Medline entries 99109950: The metabolism of 6-deoxyhexoses in bacterial and animal cells . Peptide information for frame 1 ORF from 73 bp to 1074 bp; peptide length: 334 Category: similarity to known protein 1 MVGREKELSI HFVPGSCRLV EEEVNIPNRR VLVTGATGLL GRAVHKEFQQ 51 NNWHAVGCGF RRARPKFEQV NLLDSNAVHH IIHDFQPHVI VHCAAERRPD 101 VVENQPDAAS QLNVDASGNL AKEAAAVGAF LIYISSDYVF DGTNPPYREE 151 DIPAPLNLYG KTKLDGEKAV LENNLGAAVL RIPILYGEVE KLEESAVTVM 201 FDKVQFSNKS ANMDHWQQRF PTHVKDVATV CRQLAEKRML DPSIKGTFHW 251 SGNEQMTKYE MACAIADAFN LPSSHLRPIT DSPVLGAQRP RNAQLDCSKL 301 ETLGIGQRTP FRIGIKESLW PFLIDKRWRQ TVFH BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_6b24, frame 1 PIR:T00104 probable dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133) - Actinobacillus actinomycetemcomitans, N = 1, Score = 293, P = 6.4e-26 TREMBL :SSU51197_21 gene: "rhsD"; product: "dTDP-6-deoxy-L-mannose-dehydrogenase"; Sphingomonas S88 sphingan polysaccharide synthesis (spsG) , (spsS), (spsR) , glycosyl transferase (spsQ) , (spsl), glycosyl transferase (spsK), glycosyl transferase (spsL), (sps ), (spsF), (spsD), (spsC), (spsE), Urf 32, Urf 26, ATP-bindmg cassette trans>., N = 1, Score = 291, P = le-25 SWISSPROT :RFBD_RHISN PROBABLE DTDP-4-DEHYDRORHAMNOSE REDUCTASE (EC 1.1.1.133) (DTDP-4-KETO- L-RHAMNOSE REDUCTASE) (DTDP-6-DEOXY-L-MANNOSE DEHYDROGENASE) (DTDP-L- RHAMNOSE SYNTHETASE) . , N = 1, Score = 283, P = 7.4e-25 >PIR:T00104 probable dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133) - Actinobacillus actinomycetemcomitans Length = 294 HSPs: Score = 293 (44.0 bits), Expect = 6.4e-26, P = 6.4e-26 Identities = 89/276 (32%), Positives = 151/276 (54%) Query: 30 RVLVTGATGLLGRAVHKEFQQNNWHAVGCGFRRARPKFEQVNLLDSNAVHHIIHDFQPHV 89 R+L+TGA G LGR++ K N + V F ++++ + + V II F+P+V Sbjct: 3 RLLITGAGGQLGRSLAKLLVDNGRYEV LALDFSELDITNKDMVFSIIDSFKPNV 56 Query: 90 IVHCAAERRPDVVENQPDAASQLNVDASGNLAKEAAAVGAFLIYIΞSDYVFDG-TNPPYR 148 I++ AA D E + +A +NV LA+ A + ++++S+DYVFDG + Y+ Sbjct: 57 IINAAAYTSVDQAELEVSSAYSVNVRGVQYLAEAAIRHNSAILHVSTDYVFDGYKSGKYK 116 Query: 149 EEDIPAPLNLYGKTKLDGEKAVLENNLGAAVLRIPILYGEVEKLEESAVTVMFDKVQFSN 208 E Dl PL +YGK+K +GE+ +L + + +LR +GE + V M ++ + Sbjct: 117 ETDIIHPLCVYGKΞKAEGERLLLTLSPKSIILRTΞWTFGEYGN NFVKTML-RLAKNR 172 Query: 209 KSANMDHWQQRFPTHVKDVATVCRQLAEKRMLDPSIK-GTFHWSGNEQMTKYEMACAIAD 267 + Q PT+ D+A+V Q+AEK ++ ++K G +H++G ++ Y+ A Al D Sbjct: 173 DILGVVADQIGGPTYSGDIASVLIQIAEKIIVGETVKYGIYHFTGEPCVSWYDFAIAIFD 232 Query: 268 AF NLPSSHLRPITDSPVLGAQRPRNAQLDCSKLE-TLGI 305 N+P + D P L A+RP N+ LD +K++ GI Sbjct: 233 EAVAQKVLENVPLVNAITTADYPTL-AKRPANSCLDLTKIQQAFGI 277 Pedant information for DKFZphfbr2_6b24, frame 1 Report for DKFZphfbr2_6b24.1 [LENGTH] 334 [MW] 37551.98 [pi] 6.90 [HOMOL] PIR:T00104 probable dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133) - Actinobacillus actinomycetemcomitans 6e-25 [FUNCAT] 01.06.01 lipid, fatty-acid and sterol biosynthesis [S. cerevisiae, YGLOOlc] 6e-04 [EC] 1.1.1.133 dTDP-4-dehydrorhamnose reductase 2e-16 [PIRKW] lipopolysacchaπde biosynthesis 2e-16 [PIRKW] NADP 2e-16 [PIRKW] oxidoreductase 2e-16 [PIRKW] streptomycin biosynthesis le-19 [SUPFAM] dTDP-dihydrostreptose synthase le-20 [PROSITE] MYRISTYL 1 [PROSITE] CK2_PHOSPHO_SITE 4 [PROSITE] PKC_PHOSPHO_SITE 3 [PROSITE] ASN_GLYCOSYLATION 1 [KW] Alpha_Beta SEQ MVGREKELSIHFVPGSCRLVEEEVNIPNRRVLVTGATGLLGRAVHKEFQQNNWHAVGCGF PRD cccccceeeccccccceeeeecccccccceeeeeccccchhhhhhhhhhhccceeeeecc SEQ RRARPKFEQVNLLDSNAVHHIIHDFQPHVIVHCAAERRPDVVENQPDAASQLNVDASGNL PRD cccccccccccccchhhhhhhhhhhccceeeehhhhhhhhhhhhhhhhhhhhhhccchhh SEQ AKEAAAVGAFLIYISSDYVFDGTNPPYREEDIPAPLNLYGKTKLDGEKAVLENNLGAAVL PRD hhhhhhhhheeeeeeccccccccccccccccccccccccchhhhhhhhhccccccceeee SEQ RIPILYGEVEKLEESAVTVMFDKVQFSNKSANMDHWQQRFPTHVKDVATVCRQLAEKRML PRD eeeeeecccccccchhhhhhhhhhhhhccceeeccccccccccchhhhhhhhhhhhhhhh SEQ DPSIKGTFHWSGNEQMTKYEMACAIADAFNLPSSHLRPITDSPVLGAQRPRNAQLDCSKL PRD cccccceeeeccccccchhhhhhhhhhhhhcccccccccccccccccccccccchhhhhh SEQ ETLGIGQRTPFRIGIKESLWPFLIDKRWRQTVFH PRD hhhhccccchhhhhhhhhhhhhhhhhhhhhcccc Prosite for DKFZphfbr2_6b24.1 PS00001 208->212 ASN_GLYCOSYLATION PDOC00001 PS00005 16->19 PKC_PHOSPHO_ΞITE PDOC00005 PS00005 207->210 PKC_PHOSPHO_SITE PDOC00005 PS00005 243->246 PKC_PHOSPHO_SITE PDOC00005 PS00006 162->166 CK2_PHOSPHO_SITE PDOC00006 PS00006 251->255 CK2_PHOSPHO_SITE PDOC00006 PS00006 257->261 CK2_PHOSPHO_SITE PDOC00006 PS00006 298->302 CK2_PHOSPHO_SITE PDOC00006 PS00008 314->320 MYRISTYL PDOC00008 (No Pfam data available for DKFZphfbr2_6b24.1) DKFZphfbr2_6ι20 group: brain derived DKFZphfbr2_6ι20 encodes a novel 296 ammo acid protein with similarity to ribosomal protein L15 precursor of S. cerevisiae mitochondria. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of brain-specific genes . similarity to ribosomal protein L15 precursor, mitochondrial complete cDNA, complete eds, EST hits potential miochondrial L15 ribosomal protein Sequenced by AGOWA Locus: /map="377.5 cR from top of Chr8 linkage group" Insert length: 1122 bp Poly A stretch at pos. 1099, polyadenylation signal at pos. 1071 1 GGGGGCCCTT GAAAGTTCTT GGATCTGCGG GTTATGGCCG GTCCCTTGCA 51 GGGCGGTGGG GCCCGGGCCC TGGACCTACT CCGGGGCCTG CCGCGTGTGA 101 GCCTGGCCAA CTTAAAGCCG AATCCCGGCT CCAAGAAACC GGAGAGAAGA 151 CCAAGAGGTC GGAGAAGAGG TAGAAAATGT GGCAGAGGCC ATAAAGGAGA 201 AAGGCAAAGA GGAACCCGGC CCCGCTTGGG CTTTGAGGGA GGCCAGACTC 251 CATTTTACAT CCGAATCCCA AAATACGGGT TTAACGAAGG ACATAGTTTC 301 AGACGCCAGT ATAAGCCTAT GAGTCTCAAT AGACTGCAGT ATCTTATTGA 351 TTTGGGTCGT GTTGATCCTA GTCAACCTAT TGACTTAACC CAGCTTGTCA 401 ATGGGAGAGG TGTGACCATC CAGCCACTTA AAAGGGATTA TGATGTCCAG 451 CTGGTTGAGG AGGGTGCTGA CACCTTTACG GCAAAAGTTA ATATTGAAGT 501 ACAGTTGGCT TCAGAACTAG CTATTGCTGC CATTGAAAAA AATGGTGGTG 551 TTGTTACTAC AGCCTTCTAT GATCCAAGAA GTCTGGACAT TGTATGCAAA 601 CCTGTTCCAT TCTTTCTTCG TGGACAACCC ATTCCAAAAA GAATGCTTCC 651 ACCAGAAGAA CTGGTACCAT ATTACACTGA TGCAAAGAAC CGTGGGTACC 701 TGGCGGATCC TGCCAAATTT CCTGAAGCAC GACTTGAACT CGCCAGGAAG 751 TATGGTTATA TCTTACCTGA TATCACTAAA GATGAACTCT TCAAAATGCT 801 CTGTACTAGG AAGGATCCAA GGCAGATTTT CTTTGGTCTT GCTCCAGGAT 851 GGGTGGTGAA TATGGCCGAT AAGAAAATCC TAAAACCTAC AGATGAAAAT 901 CTCCTTAAGT ATTATACCTC ATGAATTCCC GTCCAAGGAA GCAGAGTTGT 951 TAAAGAGTAC TGGAATAGGG GCTGAAGGAT CTATATTCCC TTATTGCATT 1001 TTCCTTATGT ATAATTTTCC AGATGGTGAT GTTACTTTTC AGTGTACTCA 1051 TATGTCTCAT TTTCATCTAA AATTAAATGG CAGGAAACAA GGACTGCATA 1101 GAGAAAAAAA AAAAAAAAAA AA BLAST Results Entry HΞ500354 from database EMBL: human STS WI-12392. Length = 426 Minus Strand HSPs: Score = 1791 (268.7 bits), Expect = l.le-74, P = l.le-74 Identities = 375/384 (97%) Medline entries No Medline entry Peptide information for frame 1 ORF from 34 bp to 921 bp; peptide length: 296 Category: strong similarity to known protein 1 MAGPLQGGGA RALDLLRGLP RVSLANLKPN PGSKKPERRP RGRRRGRKCG 51 RGHKGERQRG TRPRLGFEGG QTPFYIRIPK YGFNEGHSFR RQYKPMSLNR 101 LQYLIDLGRV DPSQPIDLTQ LVNGRGVTIQ PLKRDYDVQL VEEGADTFTA 151 KVNIEVQLAS ELAIAAIEKN GGVVTTAFYD PRSLDIVCKP VPFFLRGQPI 201 PKRMLPPEEL VPYYTDAKNR GYLADPAKFP EARLELARKY GYILPDITKD 251 ELFKMLCTRK DPRQIFFGLA PGWVVNMADK KILKPTDENL LKYYTS BLASTP hits Entry S63258 from database PIR: ribosomal protein L15 precursor, mitochondrial - yeast (Saccharomyces cerevisiae) Length = 322 Score = 259 (91.2 bits), Expect = 2.0e-22, P = 2.0e-22 Identities = 71/200 (35%), Positives = 106/200 (53%) Entry H70161 from database PIR: ribosomal protein L15 (rplO) - Lyme disease spirochete Length = 145 Score = 173 (60.9 bits), Expect = 4.8e-13, P = 4.8e-13 Identities = 45/140 (32%), Positives = 73/140 (52%) Alert BLASTP hits for DKFZphfbr2_6ι20, frame 1 No Alert BLASTP hits found Pedant information for DKFZphfbr2_6ι20, frame 1 Report for DKFZphfbr2_6ι20.1 [LENGTH] 296 [MW] 33495.98 [pi] 9.98 [HOMOL] TREMBL :AF067212_1 gene: "F37F2.1"; Caenorhabditis elegans cosmid F37F2. le-3. [FUNCAT] 05.01 ribosomal proteins [S. cerevisiae, YNL284c] 7e-15 [FUNCAT] 30.16 mitochondrial organization [S. cerevisiae, YNL284C] 7e-15 [FUNCAT] j mrna translation and ribosome biogenesis [M. genitalium, MG169] le-06 [BLOCKS] BL00475D [BLOCKS] BL00475B Ribosomal protein L15 proteins [PIRKW] ribosome 2e-13 [PIRKW] mitochondrion 2e-13 [PIRKW] protein biosynthesis 2e-13 [SUPFAM] Escherichia coll ribosomal protein L15 4e-06 [PROSITE] MYRISTYL 3 [PROSITE] AMIDATION 2 [PROSITE] CK2_PHOSPHO_SITE 2 [PROSITE] PKC_PHOSPHO_SITE 4 [KW] Alpha_Beta [KW] LOW COMPLEXITY 12.50 % SEQ MAGPLQGGGARALDLLRGLPRVSLANLKPNPGΞKKPERRPRGRRRGRKCGRGHKGERQRG SEG xxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxx... PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ TRPRLGFEGGQTPFYIRlPKYGFNEGHSFRRQYKPMSLNRLQYLIDLGRVDPSQPIDLTQ SEG PRD ccccccccccccceeeeeccccccccccccccccccchhhhhhhhhccccccccccccee SEQ LVNGRGVTIQPLKRDYDVQLVEEGADTFTAKVNIEVQLASELAIAAIEKNGGVVTTAFYD SEG PRD ecccceeeeccccccceeeeeeccccccchhhhhhhhhhhhhhhhhhhhccceeeeeecc SEQ PRSLDIVCKPVPFFLRGQPIPKRMLPPEELVPYYTDAKNRGYLADPAKFPEARLELARKY SEG PRD ccccccccccccccccccccccccccccccccccccccccccccccchhhhhhhhhhhhh SEQ GYILPDITKDELFKMLCTRKDPRQIFFGLAPGWVVNMADKKILKPTDENLLKYYTS SEG PRD cccccccchhhhhhhhhcccccceeeeeccccceeeeccceeecccchhhhhcccc Prosite for DKFZphfbr2_6ι20.1 PS00005 33->36 PKC_PHOSPHO_SITE PDOC00005 PS00005 88->91 PKC PHOSPHO SITE PDOC00005 PS00005 149->152 PKC_PHOSPHO_SITE PDOC00005 PS00005 258->261 PKC_PHOSPHO_SITE PDOC00005 PS00006 248->252 CK2_PHOSPHO_SITE PDOC00006 PS00006 258->262 CK2_PHOSPHO_SITE PDOC00006 PS00008 8->14 MYRISTYL PDOC00008 PS00008 171->177 MYRISTYL PDOC00008 PS00008 268->274 MYRISTYL PDOC00008 PS00009 41->45 AMIDATION PDOC00009 PS00009 45->49 AMIDATION PDOC00009 (No Pfam data available for DKFZphfbr2_6ι20.1) DKFZphfbr2_6ol7 group: nucleic acid management DKFZphfbr2_6ol7 encodes a novel 455 ammo acid protein with strong similarity to DEAD-box ATP- dependent RNA helicases YHR065c and T26G10.1. The S. cerevisiae protein YHR065c is required for maturation of the 35S RNA primary transcript . The new protein can find application in modulating rRNA maturation. strong similar to RNA helicases complete cDNA, complete eds, EST hits probable start at Bp 27 matchs kozak consensus ANNatgG involved in maturation of r-RNA " YHR065c/Rrp3p is involved in maturation of the 35S primary transcript Drslp cold-sensitive mutation has slow 27S to 25S pre-rRNA conversion and is deficient m 60S ribosomal subunits Sequenced by AGOWA Locus : unknown Insert length: 1840 bp Poly A stretch at pos. 1815, polyadenylation signal at pos. 1793 1 GGGGACTTCC GGAGACCTCA CACAAGATGG CGGCACCCGA GGAACACGAT 51 TCTCCGACCG AAGCGTCCCA GCCGATTGTG GAAGAGGAGG AAACTAAAAC 101 ATTTAAAGAC CTGGGTGTGA CAGATGTGTT GTGTGAAGCT TGTGACCAGT 151 TGGGATGGAC AAAACCCACC AAGATTCAGA TTGAAGCTAT TCCTTTGGCC 201 TTACAAGGTC GTGATATCAT TGGGCTTGCA GAAACTGGCT CTGGAAAGAC 251 AGGCGCCTTT GCTTTGCCCA TTCTAAACGC ACTGCTGGAG ACCCCGCAGC 301 GTTTGTTTGC CCTAGTTCTT ACCCCGACTC GGGAGCTGGC CTTTCAGATC 351 TCAGAGCAGT TTGAAGCCCT GGGGTCCTCT ATTGGAGTGC AGAGTGCTGT 401 GATTGTAGGT GGAATTGATT CAATGTCTCA ATCTTTGGCC CTTGCAAAAA 451 AACCACATAT AATAATAGCA ACTCCTGGTC GACTGATTGA CCACTTGGAA 501 AATACGAAAG GTTTCAACTT GAGAGCTCTC AAATACTTGG TCATGGATGA 551 AGCCGACCGA ATACTGAATA TGGATTTTGA GACAGAGGTT GACAAGATCC 601 TCAAAGTGAT TCCTCGAGAT CGGAAAACAT TCCTCTTCTC TGCCACCATG 651 ACCAAGAAGG TTCAAAAACT TCAGCGAGCA GCTCTGAAGA ATCCTGTGAA 701 ATGTGCCGTT TCCTCTAAAT ACCAGACAGT TGAAAAATTA CAGCAATATT 751 ATATTTTTAT TCCCTCTAAA TTCAAGGATA CCTACCTGGT TTATATTCTA 801 AATGAATTGG CTGGAAACTC CTTTATGATA TTCTGCAGCA CCTGTAATAA 851 TACCCAGAGA ACAGCTTTGC TACTGCGAAA TCTTGGCTTC ACTGCCATCC 901 CCCTCCATGG ACAAATGAGT CAGAGTAAGC GCCTAGGATC CCTTAATAAG 951 TTTAAGGCCA AGGCCCGTTC CATTCTTCTA GCAACTGACG TTGCCAGCCG 1001 AGGTTTGGAC ATACCTCATG TAGATGTGGT TGTCAACTTT GACATTCCTA 1051 CCCATTCCAA GGATTACATC CATCGAGTAG GTCGAACAGC TAGAGCTGGG 1101 CGCTCCGGAA AGGCTATTAC TTTTGTCACA CAGTATGATG TGGAACTCTT 1151 CCAGCGCATA GAACACTTAA TTGGGAAGAA ACTACCAGGT TTTCCAACAC 1201 AGGATGATGA GGTTATGATG CTGACAGAAC GCGTCGCTGA AGCCCAAAGG 1251 TTTGCCCGAA TGGAGTTAAG GGAGCATGGA GAAAAGAAGA AACGCTCGCG 1301 AGAGGATGCT GGAGATAATG ATGACACAGA GGGTGCTATT GGTGTCAGGA 1351 ACAAGGTGGC TGGAGGAAAA ATGAAGAAGC GGAAAGGCCG TTAATCACTT 1401 TTATGAAGGC TCGAGTTCTG CTGTTCTGTA AAAGAAAATT GGAGAATGAA 1451 ACCTGCTCCA ACAGAGATCA TGAGACTGAA ATTGGTCAGA ATTGTGTCCA 1501 GAATGTGCTC AGCTAATTCA GTATTCTTCC CCATTCTGGG TTGGAGTTTA 1551 CTGCAGAGTA ATTCTTACAG TGCTGATGTC AAGACTGTTA CTGTTCTTCG 1601 ACTTTGATTC CTTGCTCATG ACATGAGTAG GGTGTGCTCT TCTGTCACTT 1651 CACACAGACC TTTTGCCTTT TTTAGCTGCA AGTCAAGGAC TAGGTTGATG 1701 ATGCCCATGA CCTGTAATTG TAAAGAAGCT TGGACATCTG CAAATGATAT 1751 TTAAACCATC TTGGCTTGTG CTTTATTCAA ACTAATGTGA AACAATAAAT 1801 TTAAATATTA TTTTTAAAAG AAAAAAAAAA AAAAAAAAAA BLAST Results o BLAST result Medline entries o Medlme entry Peptide information for frame 3 ORF from 27 bp to 1391 bp; peptide length: 455 Category: strong similarity to known protein 1 MAAPEEHDSP TEASQPIVEE EETKTFKDLG VTDVLCEACD QLGWTKPTKI 51 QIEAIPLALQ GRDIIGLAET GSGKTGAFAL PILNALLETP QRLFALVLTP 101 TRELAFQISE QFEALGSSIG VQSAVIVGGI DSMSQSLALA KKPHIIIATP 151 GRLIDHLENT KGFNLRALKY LVMDEADRIL NMDFETEVDK ILKVIPRDRK 201 TFLFSATMTK KVQKLQRAAL KNPVKCAVSS KYQTVEKLQQ YYIFIPSKFK 251 DTYLVYILNE LAGNSFMIFC STCNNTQRTA LLLRNLGFTA IPLHGQMSQS 301 KRLGSLNKFK AKARSILLAT DVASRGLDIP HVDVVVNFDI PTHSKDYIHR 351 VGRTARAGRΞ GKAITFVTQY DVELFQRIEH LIGKKLPGFP TQDDEVMMLT 401 ERVAEAQRFA RMELREHGEK KKRΞREDAGD NDDTEGAIGV RNKVAGGKMK 451 KRKGR BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_6ol7, frame 3 PIR:S40731 ATP-dependent RNA helicase homolog T26G10.1 - Caenorhabditis elegans, N = 1, Score = 1497, P = 1.6e-153 PIR:S46713 hypothetical protein YHR065c - yeast (Saccharomyces cerevisiae), N = 1, Score = 1154, P = 3.6e-117 TREMBL :ATH010462_1 gene: "RH10"; product: "RNA helicase"; Arabidopsis thaliana mRNA for DEAD box RNA helicase, RH10, N = 1, Score = 1122, P = 8.9e-114 TREMBL :AC002985_2 product: "R27090_2"; Human DNA from chromosome 19-specιfιc cosmid R27090, genomic sequence, complete sequence., N = 1, Score = 950, P = 1.5e-95 >PIR:S40731 ATP-dependent RNA helicase homolog T26G10.1 - Caenorhabditis elegans Length = 489 HSPs: Score = 1497 (224.6 bits), Expect = 1.6e-153, P = 1.6e-153 Identities = 283/442 (64%), Positives = 364/442 (82%) Query: 19 EEEETKTFKDLGVTDVLCEACDQLGWTKPTKIQIEAIPLALQGRDIIGLAETGSGKTGAF 78 E+ + K+F +LGV+ LC+AC +LGW KP+KIQ A+P ALQG+D+IGLAETGSGKTGAF Sbjct: 39 EDVKEKSFAELGVSQPLCDACQRLGWMKPSKIQQAALPHALQGKDVIGLAETGSGKTGAF 98 Query: 79 ALPILNALLETPQRLFALVLTPTRELAFQISEQFEALGSSIGVQSAVIVGGIDSMSQΞLA 138 A+P+L +LL+ PQ F LVLTPTRELAFQI +QFEALGS IG+ +AVIVGG+D +Q++A Sbjct: 99 AIPVLQSLLDHPQAFFCLVLTPTRELAFQIGQQFEALGSGIGLIAAVIVGGVDMAAQAMA 158 Query: 139 LAKKPHIIIATPGRLIDHLENTKGFNLRALKYLVMDEADRILNMDFETEVDKILKVIPRD 198 LA++PHII+ATPGRL+DHLENTKGFNL+ALK+L+MDEADRILNMDFE E+DKILKVIPR+ Sbjct: 159 LARRPHIIVATPGRLVDHLENTKGFNLKALKFLIMDEADRILNMDFEVELDKILKVIPRE 218 Query: 199 RKTFLFSATMTKKVQKLQRAALKNPVKCAVSSKYQTVEKLQQYYIFIPSKFKDTYLVYIL 258 R+T+LFSATMTKKV KL+RA+L++P + +VSS+Y+TV+ L+Q+YIF+P+K+K+TYLVY+L Sbjct: 219 RRTYLFSATMTKKVSKLERASLRDPARVSVSSRYKTVDNLKQHYIFVPNKYKETYLVYLL 278 Query: 259 NELAGNSFMIFCSTCNNTQRTALLLRNLGFTAIPLHGQMSQSKRLGΞLNKFKAKARSILL 318 NE AGNS ++FC+TC T + A++LR LG A+PLHGQMΞQ KRLGSLNKFK+KAR IL+ Sbjct: 279 NEHAGNSAIVFCATCATTMQIAVMLRQLGMQAVPLHGQMSQEKRLGSLNKFKSKAREILV 338 Query: 319 ATDVASRGLDIPHVDVVVNFDIPTHSKDYIHRVGRTARAGRSGKAITFVTQYDVELFQRI 378 TDVA+RGLDIPHVD+V+N+D+P+ SKDY+HRVGRTARAGRSG AIT VTQYDVE +Q+I Sbjct: 339 CTDVAARGLDIPHVDMVINYDMPSQSKDYVHRVGRTARAGRSGIAITVVTQYDVEAYQKI 398 Query: 379 EHLIGKKLPGFPTQDDEVMMLTERVAEAQRFARMELREHGEKKK RSREDAGDNDD 433 E +GKKL + ++EVM+L ER EA AR+E++E EKKK R +D GD ++ Sbjct: 399 EANLGKKLDEYKCVENEVMVLVERTQEATENARIEMKEMDEKKKSGKKRRQNDDFGDTEE 458 Query: 434 TEGAIGVRNKVAGGKMKKRKGR 455 + G + K GG+ GR Sbjct: 459 SGGRFKMGIKSMGGRGGSGGGR 480 Pedant information for DKFZphfbr2_6ol7, frame 3 Report for DKFZphfbr2_6ol7.3 [LENGTH] 455 [MW] 50646.80 [pi] 9.18 [HOMOL] PIR:S40731 ATP-dependent RNA helicase homolog T26G10.1 - Caenorhabditis elegans le-167 [FUNCAT] 04.01.04 rrna processing [S. cerevisiae, YHR065C] le-127 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YHR065c] le-127 [FUNCAT] 04.99 other transcription activities [Ξ. cerevisiae, YHR169w] 2e-79 [FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YLLOOδw] le-71 [FUNCAT] 04.05.01.07 chromatin modification [S. cerevisiae, YMR290c] 4e-66 [FUNCAT] j mrna translation and ribosome biogenesis [H. influenzae, HI0231 RNA] le-63 [FUNCAT] 09.01 biogenesis of cell wall [S. cerevisiae, YJL033w] le-58 [FUNCAT] 04.05.03 mrna processing (splicing) [S. cerevisiae, YDL084w] le-55 [FUNCAT] 05.04 translation (initiation, elongation and termination) [S. cerevisiae, YOR204w] 5e- 55 [ FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YOR204w] 5e-55 [FUNCAT] 1 genome replication, transcription, recombination and repair [H. influenzae, HI0892] 9e-48 [FUNCAT] 98 classification not yet clear-cut [S. cerevisiae, YLR276c] 2e-45 [FUNCAT] 30.16 mitochondrial organization [S. cerevisiae, YDR194c] 4e-42 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YGL064c] 7e-16 [ FUNCAT ] 03.19 recombination and dna repair [S. cerevisiae, YMR190c] 7e-12 [FUNCAT] 11.10 cell death [S. cerevisiae, YMR190c] 7e-12 [ FUNCAT ] r general function prediction [M. jannaschn, MJ1401] 5e-06 [BLOCKS] BL00175B Phosphoglycerate mutase family phosphohistidme proteins [BLOCKS] BL00039D DEAD-box subfamily ATP-dependent helicases proteins [BLOCKS] BL00039C DEAD-box subfamily ATP-dependent helicases proteins [BLOCKS] BL00039B DEAD-box subfamily ATP-dependent helicases proteins [BLOCKS] BL00039A DEAD-box subfamily ATP-dependent helicases proteins [PIRKW] nucleus 4e-60 [PIRKW] RNA binding 7e-69 [PIRKW] DEAD box 7e-69 [PIRKW] transmembrane protein 9e-41 [PIRKW] DNA binding 3e-55 [PIRKW] recF recombination pathway 3e-ll [PIRKW] ATP le-126 [PIRKW] purme nucleotide binding 7e-69 [PIRKW] P-loop le-126 [PIRKW] hydrolase le-55 [PIRKW] protein biosynthesis 7e-69 [PIRKW] ATP binding 3e-61 [SUPFAM] ATP-dependent RNA helicase eIF-4A 8e-06 [SUPFAM] WW repeat homology 4e-58 [SUPFAM] translation initiation factor eIF-4A 7e-69 [SUPFAM] DEAD/H box helicase homology le-126 [SUPFAM] recQ helicase homology 5e-12 [SUPFAM] ATP-dependent RNA helicase homology 8e-06 [SUPFAM] unassigned DEAD/H box helicases le-126 [SUPFAM] ATP-dependent RNA helicase DBP1 4e-60 [SUPFAM] ATP-dependent RNA helicase DHH1 le-58 [SUPFAM] recQ protein 3e-ll [SUPFAM] tobacco ATP-dependent RNA helicase DB10 4e-58 [SUPFAM] Bloom's syndrome helicase 5e-12 [PROSITE] DEAD_ATP_HELICASE 1 [PROSITE] ATP_GTP_A 1 [PROSITE] MYRISTYL 5 [PROSITE] AMIDATION 1 [PROSITE] CAMP_PHOSPHO_SITE 1 [PROSITE] CK2_PHOΞPHO_SITE 6 [PROSITE] PKC_PHOSPHO_SITE 9 [PROSITE] ASN_GLYCOSYLATION 1 [PFAM] Helicases conserved C-terminal domain [PFAM] DEAD and DEAH box helicases [KW] Alpha_Beta SEQ MAAPEEHDSPTEASQPIVEEEETKTFKDLGVTDVLCEACDQLGWTKPTKIQIEAIPLALQ PRD cccccccccccccccchhhhhhhhhhhccccchhhhhhhhhhcccccccccccccccccc SEQ GRDIIGLAETGSGKTGAFALPILNALLETPQRLFALVLTPTRELAFQISEQFEALGSSIG PRD ccceeeeeccccccceeehhhhhhhhcccccceeeeeeccchhhhhhhhhhhhhhhhhcc SEQ VQSAVIVGGIDSMSQSLALAKKPHIIIATPGRLIDHLENTKGFNLRALKYLVMDEADRIL PRD eeeeeeeccchhhhhhhhhhccceeeeeccccccccccccccccccccceeehhhhhhhh SEQ NMDFETEVDKILKVIPRDRKTFLFSATMTKKVQKLQRAALKNPVKCAVSSKYQTVEKLQQ PRD hhcchhhhhhhhhhcccchhhhhhhhccchhhhhhhhhhhccceeeeeecccccchhhhh SEQ YYIFIPSKFKDTYLVYILNELAGNSFMIFCSTCNNTQRTALLLRNLGFTAIPLHGQMSQS PRD hhhhhhhhhhhhhhhhhhhhhccceeeeeeecchhhhhhhhhhhhcccceeeccccchhh SEQ KRLGSLNKFKAKARSILLATDVASRGLDIPHVDVVVNFDIPTHSKDYIHRVGRTARAGRS PRD hhhhhhhhhhhhhhhcchhhhhhhhcccccceeeeeecccccccceeeeecccccccccc SEQ GKAITFVTQYDVELFQRIEHLIGKKLPGFPTQDDEVMMLTERVAEAQRFARMELREHGEK PRD cceeeeeecchhhhhhhhhhhhhhhhccccccchhhhhhhhhhhhhhhhhhhhhhhhhhh SEQ KKRSREDAGDNDDTEGAIGVRNKVAGGKMKKRKGR PRD hhhhccccccccccccccccccccccccccccccc Prosite for DKFZphfbr2_6ol7.3 PS00001 274->278 ASN_GLYCOSYLATION PDOC00001 PS00004 421->425 CAMP_PHOSPHO_SITE PDOC00004 PS00005 25->28 PKC_PHOSPHO_SITE PDOC00005 PS00005 72->75 PKC_PHOSPHO_SITE PDOC00005 PS00005 209->212 PKC_PHOSPHO_SITE PDOC00005 PS00005 229->232 PKC_PHOSPHO_SITE PDOC00005 PS00005 276->279 PKC_PHOSPHO_SITE PDOC00005 PS00005 300->303 PKC_PHOSPHO_SITE PDOC00005 PS00005 354->357 PKC_PHOSPHO_SITE PDOC00005 PS00005 360->363 PKC_PHOSPHO_SITE PDOC00005 PS00005 400->403 PKC_PHOSPHO_SITE PDOC00005 PS00006 9->13 CK2_PHOSPHO_SITE PDOC00006 PS00006 25->29 CK2_PHOSPHO_SITE PDOC00006 PS00006 186->190 CK2_PHOSPHO_SITE PDOC00006 PS00006 368->372 CK2_PHOSPHO_SITE PDOC00006 PS00006 391->395 CK2_PHOSPHO_SITE PDOC00006 PS00006 424->428 CK2_PHOSPHO_SITE PDOC00006 PS00008 66->72 MYRISTYL PDOC00008 PS00008 71->77 MYRISTYL PDOC00008 PS00008 116->122 MYRISTYL PDOC00008 PS00008 120->126 MYRISTYL PDOC00008 PS00008 128->134 MYRISTYL PDOC00008 PS00009 382->386 AMIDATION PDOC00009 PS00017 68->76 ATP_GTP_A PDOC00017 PS00039 172->181 DEAD ATP HELICASE PDOC00039 Pfam for DKFZphfbr2_6ol7.3 HMM_NAME DEAD and DEAH box helicases HMM *gLpPWILRnIyeMGFEkPTPIQQqAIPιILeGRDVMACAQTGSGKTAAF G ++ ++++++++G++KPT+IQ +AIP++L+GRD+++ A TGSGKT+AF Query 30 GVTDVLCEACDQLGWTKPTKIQIEAIPLALQGRDIIGLAETGSGKTGAF HMM HPMLQHIDwdPWpqpPQdPrALILAPTRELAMQIQEEcRkFgkHMnglR ++P+L ++++P + ++AL+L+PTRELA QI+E+++++G++++ ++ Query 79 ALPILNALLETP QR-LFALVLTPTRELAFQISEQFEALGSSIG-VQ 122 HMM ImcIYGGtnMRdQMRmLeRGpPHIVIATPGRLIDHIER.gtldLDrleML +++I+GG + + Q L+++P HI+IATPGRLIDH+E+ ++L+++++L Query 123 SAVIVGGIDSMSQSLALAKKP-HIIIATPGRLIDHLENTKGFNLRALKYL 171 HMM VMDEADRMLDMGFIDQIRrlMrqIPMpwNRQTMMFSATMPdelqELARrF VMDEADR+L+M+F+ ++++I++ IP ++R T +FSATM++++Q+L+R+ Query 172 VMDEADRILNMDFETEVDKILKVIP—RDRKTFLFSATMTKKVQKLQRAA 219 HMM MRNPIRInldMdElTtnEnlkQwYiyVerEMWKfdcLcrLIe* ++NP+ ++ ++++T++ ++Q+YI+++ + K +L+++++ Query 220 LKNPVKCAVSSKYQTVE-KLQQYYIFIP-SKFKDTYLVYILN 259 HMM_NAME Helicases conserved C-terminal domain HMM *EιleeWLknlGIrvmYIHGdMpQeERdeIMddFNnGEynVLIcTDVggR ++ + L+NLG++++ +HG+M+Q +R+ +++F++ +L++TDV++R Query 277 QRTALLLRNLGFTAIPLHGQMSQSKRLGSLNKFKAKARSILLATDVAΞR 325 HMM GIDIPdVNHVINYDMPWNPEqYIQRIGRTgRIG* G+DIP V++V+N+D+P ++ +YI+R+GRT+R+G Query 326 GLDIPHVDVVVNFDIPTHSKDYIHRVGRTARAG 358 DKFZphfbr2_71o20 group: brain derived DKFZphfbr2_71o20 encodes a novel 232 amino acid protein without similarity to known proteins. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of brain-specific genes . unknown complete cDNA, complete eds, EST hits on genomic level encoded by AC006186 (3 exons) Sequenced by GBF Locus: /map="10q22.1" Insert length: 1768 bp Poly A stretch at pos. 1742, polyadenylation signal at pos. 1726 1 GGGGGCAGCA GGCCAAGGGG GAGGTGCGAG CGTGGACCTG GGACGGGTCT 51 GGGCGGCTCT CGGTGGTTGG CACGGGTTCG CACACCCATT CAAGCGGCAG 101 GACGCACTTG TCTTAGCAGT TCTCGCTGAC CGCGCTAGCT GCGGCTTCTA 151 CGCTCCGGCA CTCTGAGTTC ATCAGCAAAC GCCCTGGCGT CTGTCCTCAC 201 CATGCCTAGC CTTTGGGACC GCTTCTCGTC GTCGTCCACC TCCTCTTCGC 251 CCTCGTCCTT GCCCCGAACT CCCACCCCAG ATCGGCCGCC GCGCTCAGCC 301 TGGGGGTCGG CGACCCGGGA GGAGGGGTTT GACCGCTCCA CGAGCCTGGA 351 GAGCTCGGAC TGCGAGTCCC TGGACAGCAG CAACAGTGGC TTCGGGCCGG 401 AGGAAGACAC GGCTTACCTG GATGGGGTGT CGTTGCCCGA CTTCGAGCTG 451 CTCAGTGACC CTGAGGATGA ACACTTGTGT GCCAACCTGA TGCAGCTGCT 501 GCAGGAGAGC CTGGCCCAGG CGCGGCTGGG CTCTCGACGC CCTGCGCGCC 551 TGCTGATGCC TAGCCAGTTG GTAAGCCAGG TGGGCAAAGA ACTACTGCGC 601 CTGGCCTACA GCGAGCCGTG CGGCCTGCGG GGGGCGCTGC TGGACGTCTG 651 CGTGGAGCAG GGCAAGAGCT GCCACAGCGT GGGCCAGCTG GCACTCGACC 701 CCAGCCTGGT GCCCACCTTC CAGCTGACCC TCGTGCTGCG CCTGGACTCA 751 CGACTCTGGC CCAAGATCCA GGGGCTGTTT AGCTCCGCCA ACTCTCCCTT 801 CCTCCCTGGC TTCAGCCAGT CCCTGACGCT GAGCACTGGC TTCCGAGTCA 851 TCAAGAAGAA GCTGTACAGC TCGGAACAGC TGCCCATTGA GGAGTGTTGA 901 ACTTCAACCT GAGGGGGCCG ACAGTGCCCT CCAAGACAGA GACGACTGAA 951 CTTTTGGGGT GGAGACTAGA GGCAGGAGCT GAGGGACTGA TTCCAGTGGT 1001 TGGAAAACTG AGGCAGCCAC CTAAAGTGGA GGTGGGGGAA TAGTGTTTCC 1051 CAGGAAGCTC ATTGAGTTGT GTGCGGGTGG CTGTGCATTG GGGACACATA 1101 CCCCTCAGTA CTGTAGCATG AAACAAAGGC TTAGGGGCCA ACAAGGCTTC 1151 CAGCTGGATG TGTGTGTAGC ATGTACCTTA TTATTTTTGT TACTGACAGT 1201 TAACAGTGGT GTGACATCCA GAGAGCAGCT GGGCTGCTCC CGCCCCAGCC 1251 TGGCCCAGGG TGAAGGAAGA GGCACGTGCT CCTCAGAGCA GCCGGAGGGA 1301 AGGGGGAGGT CGGAGGTCGT GGAGGTGGTT TGTGTATCTT ACTGGTCTGA 1351 AGGGACCAAG TGTGTTTGTT GTTTGTTTTG TATCTTGTTT TTCTGATCGG 1401 AGCATCACTA CTGACCTGTT GTAGGCAGCT ATCTTACAGA CGCATGAATG 1451 TAAGAGTAGG AAGGGGTGGG TGTCAGGGAT CACTTGGGAT CTTTGACACT 1501 TGAAAAATTA CACCTGGCAG CTGCGTTTAA GCCTTCCCCC ATCGTGTACT 1551 GCAGAGTTGA GCTGGCAGGG GAGGGGCTGA GAGGGTGGGG GCTGGAACCC 1601 CTTCCCGGGA GGAGTGCCAT CTGGGTCTTC CATCTAGAAC TGTTTACATG 1651 AAGATAAGAT ACTCACTGTT CATGAATACA CTTGATGTTC AAGTATTAAG 1701 ACCTATGCAA TATTTTTTAC TTTTCTAATA AACATGTTTG TTAAAACAAA 1751 AAAAAAAAAA AAAAAAAA BLAST Results Entry AC006186 from database EMBLNEW: *** SEQUENCING IN PROGRESS *** Homo sapiens chromosome 10 clone CRI-JC2048 map 10q22.1; HTGS phase 1, 4 unordered pieces. Score = 6512, P = O.Oe+00, identities = 1326/1345 3 exons Medlme entries No Medlme entry Peptide information for frame 1 ORF from 202 bp to 897 bp; peptide length: 232 Category: putative protein 1 MPSLWDRFSS SSTSSSPSSL PRTPTPDRPP RSAWGSATRE EGFDRSTSLE 51 SSDCESLDSS NSGFGPEEDT AYLDGVSLPD FELLSDPEDE HLCANLMQLL 101 QESLAQARLG SRRPARLLMP SQLVSQVGKE LLRLAYSEPC GLRGALLDVC 151 VEQGKSCHSV GQLALDPSLV PTFQLTLVLR LDSRLWPKIQ GLFSSANSPF 201 LPGFSQSLTL STGFRVIKKK LYSSEQLPIE EC BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_71o20, frame 1 No Alert BLASTP hits found Pedant information for DKFZphfbr2_71o20, frame 1 Report for DKFZphfbr2_71o20.1 [LENGTH] 232 [MW] 25354.60 [pi] 4.87 [PROSITE] MYRISTYL 2 [PROSITE] CK2_PHOSPHO_SITE 6 [PROSITE] GLYCOSAMINOGLYCAN 1 [PROSITE] PKC_PHOSPHO_SITE 1 [KW] All_Alpha [KW] LOW COMPLEXITY 17.67 SEQ MPSLWDRFSSSSTSΞSPSSLPRTPTPDRPPRSAWGSATREEGFDRSTSLESSDCESLDSS SEG xxxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxx PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ NSGFGPEEDTAYLDGVSLPDFELLΞDPEDEHLCANLMQLLQESLAQARLGSRRPARLLMP SEG XX PRD cccccccccccccccccccceeeccccccchhhhhhhhhhhhhhhhhhccccccceeecc SEQ SQLVSQVGKELLRLAYSEPCGLRGALLDVCVEQGKSCHSVGQLALDPSLVPTFQLTLVLR SEG PRD ccccchhhhhhhhhhhcccccchhhhhhhhccccccccccccccccccccchhhhhhccc SEQ LDSRLWPKIQGLFSSANSPFLPGFSQSLTLSTGFRVIKKKLYSSEQLPIEEC SEG PRD cccccccccccccccccccccccccceeeecccccccccccccccccccccc Prosite for DKFZphfbr2_71o20.1 PS00002 62->66 GLYCOSAMINOGLYCAN PDOC00002 PS00005 111->114 PKC_PHOSPHO_SITE PDOC00005 PS00006 3->7 CK2_PHOSPHO_SITE PDOC00006 PS00006 38->42 CK2_PHOSPHO_SITE PDOC00006 PS00006 47->51 CK2_PHOSPHO_SITE PDOC00006 PS00006 52->56 CK2_PHOΞPHO_SI E PDOC00006 PS00006 77->81 CK2_PHOSPHO_SITE PDOC00006 PS00006 85->89 CK2_PHOSPHO_SITE PDOC00006 PS00008 141->147 MYRISTYL PDOC00008 PS00008 191->197 MYRISTYL PDOC00008 (No Pfam data available for DKFZphfbr2_71o20.1) DKFZphfbr2_72bl8 group: nucleic acid management DKFZphfbr2_72bl8 encodes a novel 715 amino acid protein w th similarity to E. coli DNA-damage- mducibile protein dmP and other proteins induced by DNA-damage. The novel protein is similar to dinP of E. coli, yqjH of B. subtilis, dinP of M. tuberculosis and T19K24.15 of A. thaliana. The dinB/P pathway is a second SOS-pathway in E. coli. Therefore the new gene seems to be involved in DNA repair. The new protein can find application in modulating DNA repair and mutagenesis. similarity to DNA damage induced genes complete cDNA, complete eds, potential start at Bp 49, EST hits localisation primer site B is missing 1 Sequenced by LMU Locus: /map="416.0 cR from top of Chrl8 linkage group"?? Insert length: 2475 bp Poly A stretch at pos. 2452, polyadenylation signal at pos. 2431 1 GGGGGAGGAA GGCGGCGGCG ACGACGAGGA AGACGCCGAG GCCTGGGCCA 51 TGGAACTGGC GGACGTGGGG GCGGCAGCCA GCTCGCAGGG AGTTCATGAT 101 CAAGTGTTGC CCACACCAAA TGCTTCATCC AGAGTCATAG TACATGTGGA 151 TCTGGATTGC TTTTATGCAC AAGTAGAAAT GATCTCAAAT CCAGAGCTAA 201 AAGACAAACC TTTAGGGGTT CAACAGAAAT ATTTGGTGGT TACCTGCAAC 251 TATGAAGCTA GGAAACTTGG AGTTAAGAAA CTTATGAATG TCAGAGATGC 301 AAAAGAAAAG TGTCCACAGT TGGTATTAGT TAATGGAGAA GACCTGACCC 351 GCTACAGAGA AATGTCTTAT AAGGTTACAG AATTACTGGA AGAATTTAGT 401 CCAGTTGTTG AGAGACTTGG ATTTGATGAA AATTTTGTGG ATCTAACAGA 451 AATGGTTGAG AAGAGACTAC AGCAGCTGCA AAGTGATGAA CTTTCTGCGG 501 TGACTGTGTC GGGTCATGTA TACAATAATC AGTCTATAAA CCTGCTTGAC 551 GTCTTGCACA TCAGACTACT TGTTGGATCT CAGATTGCAG CAGAGATGCG 601 GGAAGCCATG TATAATCAGT TGGGGCTCAC TGGCTGTGCT GGAGTGGCTT 651 CTAATAAACT GTTGGCAAAA TTAGTTTCTG GTGTCTTTAA ACCAAATCAA 701 CAAACAGTCT TATTACCTGA AAGTTGTCAA CATCTTATTC ATAGTTTGAA 751 TCACATAAAG GAAATACCTG GTATTGGCTA TAAAACTGCC AAATGTCTTG 801 AAGCACTGGG TATCAATAGT GTGCGTGATC TCCAAACCTT TTCACCCAAA 851 ATTTTAGAAA AAGAATTAGG AATTTCAGTT GCTCAGCGTA TCCAAAAGCT 901 CAGTTTTGGA GAGGATAACT CCCCTGTGAT ACTCTCAGGA CCACCTCAGT 951 CCTTTAGTGA AGAAGATTCA TTTAAAAAAT GTACATCTGA AGTTGAAGCT 1001 AAAAATAAGA TTGAAGAACT ACTTGCTAGT CTTTTAAACA GAGTATGCCA 1051 AGATGGAAGG AAGCCTCATA CAGTGAGATT AATAATCCGT CGGTATTCCT 1101 CTGAGAAGCA CTATGGTCGT GAGAGTCGTC AGTGCCCTAT TCCTTCACAT 1151 GTAATTCAGA AATTAGGGAC AGGAAATTAT GATGTGATGA CCCCAATGGT 1201 TGATATACTT ATGAAACTTT TTCGAAATAT GGTGAATGTG AAGATGCCAT 1251 TTCACCTTAC CCTTCTAAGT GTGTGCTTCT GCAACCTTAA AGCACTAAAT 1301 ACTGCTAAGA AAGGGCTTAT TGATTATTAT TTAATGCCAT CATTATCAAC 1351 TACTTCACGC TCTGGCAAGC ACAGTTTTAA AATGAAAGAC ACTCATATGG 1401 AAGATTTTCC CAAAGACAAA GAAACAAACC GGGATTTCCT ACCAAGTGGA 1451 AGAATTGAAA GTACAAGAAC TAGGGAGTCT CCACTAGATA CCACAAATTT 1501 TTCTAAAGAA AAAGACATTA ATGAATTCCC ACTCTGTTCA CTTCCTGAAG 1551 GTGTTGACCA AGAAGTCTCC AAGCAGCTTC CAGTAGATAT TCAAGAAGAA 1601 ATCCTTTCTG GAAAATCTAG GGAAAAATTT CAAGGGAAAG GAAGTGTGAG 1651 TTGTCCATTA CATGCCTCTA GAGGAGTATT ATCTTTCTTT TCTAAAAAAC 1701 AAATGCAAGA TATTCCCATA AATCCTAGAG ATCATTTATC CAGTAGCAAA 1751 CAGGTATCCT CTGTATCTCC TTGTGAACCG GGAACATCAG GCTTTAATAG 1801 CAGTAGTTCT TCTTACATGT CTAGCCAAAA GGATTATTCA TATTATTTAG 1851 ATAATAGATT AAAAGATGAA CGAATAAGTC AAGGACCTAA AGAACCTCAA 1901 GGATTCCACT TTACAAATTC AAACCCTGCT GTGTCTGCTT TTCATTCATT 1951 TCCAAACTTG CAGAGTGAGC AACTTTTCTC CAGAAACCAC ACTACAGATA 2001 GCCATAAGCA AACAGTAGCA ACAGACTCTC ATGAAGGACT TACAGAAAAT 2051 AGAGAGCCAG ATTCTGTTGA TGAGAAAATT ACTTTCCCTT CTGACATTGA 2101 TCCTCAAGTT TTCTATGAAC TACCAGAAGC AGTACAAAAG GAACTGCTGG 2151 CAGAGTGGAA GAGAACAGGA TCAGATTTCC ACATTGGACA TAAATAAGCA 2201 TATTCAGCAA AAAGGTCTGA AAAGCAAGGG AATACCATTA TTTTCGGATT 2251 AGCGGTTTAT TAAGCTCTTC TATATTAAAC ACTAATAGAT ATTCAATAAC 2301 GGAGTAAACT GTTCCAGATA AAGCAAGAAT AGTTGCAAGA AGTAAATTCT 2351 GGCACAAAGC GTAAAAATAT AACAGAAGAA ATAATGTAAA ATACTATCTT 2401 TTATGTCTAA AGCCATTTTA TATTACTTTT CAATAAAAAG AATATCATGG 2451 TCAAAAAAAA AAAAAAAAAA AAAAC BLAST Results Entry HS086339 from database EMBL: human STS WI-11064. Score = 1523, P = 3.0e-64, identities = 327/343 Medline entries No Medline entry Peptide information for frame 2 ORF from 50 bp to 2194 bp; peptide length: 715 Category: similarity to known protein 1 MELADVGAAA SSQGVHDQVL PTPNASSRVI VHVDLDCFYA QVEMISNPEL 51 KDKPLGVQQK YLVVTCNYEA RKLGVKKLMN VRDAKEKCPQ LVLVNGEDLT 101 RYREMSYKVT ELLEEFSPVV ERLGFDENFV DLTEMVEKRL QQLQSDELSA 151 VTVSGHVYNN QSINLLDVLH IRLLVGSQIA AEMREAMYNQ LGLTGCAGVA 201 SNKLLAKLVS GVFKPNQQTV LLPESCQHLI HΞLNHIKEIP GIGYKTAKCL 251 EALGINSVRD LQTFSPKILE KELGISVAQR IQKLSFGEDN SPVILSGPPQ 301 SFSEEDSFKK CTSEVEAKNK IEELLASLLN RVCQDGRKPH TVRLIIRRYS 351 SEKHYGRESR QCPIPSHVIQ KLGTGNYDVM TPMVDILMKL FRNMVNVKMP 401 FHLTLLSVCF CNLKALNTAK KGLIDYYLMP SLSTTSRSGK HSFKMKDTHM 451 EDFPKDKETN RDFLPSGRIE STRTRESPLD TTNFSKEKDI NEFPLCSLPE 501 GVDQEVSKQL PVDIQEEILS GKSREKFQGK GSVSCPLHAS RGVLSFFSKK 551 QMQDIPINPR DHLSSSKQVS SVSPCEPGTS GFNSSSSSYM SSQKDYSYYL 601 DNRLKDERIS QGPKEPQGFH FTNSNPAVSA FHSFPNLQΞE QLFSRNHTTD 651 SHKQTVATDS HEGLTENREP DSVDEKITFP SDIDPQVFYE LPEAVQKELL 701 AEWKRTGSDF HIGHK BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_72bl8, frame 2 PIR:H64747 DNA-damage-inducibile protein dinP - Escherichia coli, N = 2, Score = 212, P = 4.2e-27 PIR:H69963 DNA-damage repair protein homolog yqjH - Bacillus subtilis, N = 2, Score = 230, P = 5.2e-26 >PIR:H69963 DNA-damage repair protein homolog yqjH - Bacillus subtilis Length = 414 HSPs: Score = 230 (34.5 bits), Expect = 5.2e-26, Sum P(2) = 5.2e-26 Identities = 47/112 (41%), Positives = 73/112 (65%) Query: 27 SRVIVHVDLDCFYAQVEMISNPELKDKPLGV QQKYLVVTCNYEARKLGVKKLMNV 81 SR+I H+D++ FYA VEM +P L+ KP+ V ++K +VVTC+YEAR GVK M V Sbjct: 5 SRIIFHIDMNSFYASVEMAYDPALRGKPVAVAGNVKERKGIVVTCSYEARARGVKTTMPV 64 Query: 82 RDAKEKCPQLVLVNGEDLTRYREMSYKVTELLEEFSPVVERLGFDENFVDLTE 134 AK CP+L+++ + RYR S + +L E++ +VE + DE ++D+T+ Sbjct: 65 WQAKRHCPELIVLP-PNFDRYRNSSRAMFTILREYTDLVEPVSIDEGYMDMTD 116 Score = 137 (20.6 bits), Expect = 5.2e-26, Sum P(2) = 5.2e-26 Identities = 43/148 (29%), Positives = 75/148 (50%) Query: 178 QIAAEMREAMYNQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNHIK 237 + A E++ + +L L G+A NK LAK+ S + KP T+L ++ L + Sbjct: 125 ETAKEIQSRLQKELLLPSSIGIAPNKFLAKMASDMKKPLGITILRKRQVPDILWPLP-VG 183 Query: 238 EIPGIGYKTAKCLEALGINSVRDLQTFSPKILEKELGIΞVAQRIQKLSFGEDNSPVILSG 297 E+ G+G KTA+ L+ LGI+++ +L L++ LGI+ R++ + G ++PV Sbjct: 184 EMHGVGKKTAEKLKGLGIHTIGELAAADEHSLKRLLGIN-GPRLKNKANGIHHAPV 238 Query: 298 PPQSFSEEDΞFKKCTSEVEAKNKIEELL 325 P+ E S ++ + EELL Sbj ct : 239 DPERIYEFKSVGNSSTLSHDSSDEEELL 266 Pedant information for DKFZphfbr2_72bl8 , frame 2 Report for DKFZphfbr2_72bl 8 . 2 [ LENGTH] 715 [MW] 80300 . 63 [pi ] 6 . 37 [HOMOL] TREMBL:SPBC16A3_11 gene: "SPBC16A3.il"; product: "hypothetical protein"; S. pombe chromosome II cosmid cl6A3. 5e-30 [FUNCAT] 11.04 dna repair (direct repair, base excision repair and nucleotide excision repair) [Ξ. cerevisiae, YDR419w] 2e-15 [FUNCAT] 1 genome replication, transcription, recombination and repair [M. gemtalium, MG360] 3e-13 [PIRKW] SOS mutagenesis 2e-ll [PIRKW] DNA repair 2e-ll [PIRKW] induced mutagenesis 2e-ll [SUPFAM] umuC protein 3e-29 [PROSITE] MYRISTYL 6 [PROSITE] AMIDATION 1 [PROSITE] CAMP_PHOSPHO_SITE 2 [PROSITE] CK 2 _PHOSPHO_SITE 15 [PROSITE] PROKAR_LIPOPROTEIN 1 [PROSITE] TYR_PHOΞPHO_SITE 2 [PROSITE] PKC_PHOSPHO_SITE 21 [PROSITE] ASN_GLYCOSYLATION 5 [KW] Alpha_Beta [KW] LOW_COMPLEXITY 4.20 % SEQ MELADVGAAASSQGVHDQVLPTPNASSRVIVHVDLDCFYAQVEMISNPELKDKPLGVQQK SEG PRD ccceeeeeeecccccceeeccccccceeeeeeeccchhhhhhhhhccccccccceeeecc SEQ YLVVTCNYEARKLGVKKLMNVRDAKEKCPQLVLVNGEDLTRYREMSYKVTELLEEFSPVV SEG PRD ceeeehhhhhhhhhhcccchhhhhhhhccceeeeccccccchhhhhhhhhhhhhhhccce SEQ ERLGFDENFVDLTEMVEKRLQQLQSDELSAVTVSGHVYNNQSINLLDVLHIRLLVGSQIA SEG PRD eeeccchhhhhhhhhhhhhhhhhhccccceeeeeccccccchhhhhhhhhhhhhhhhhhh SEQ AEMREAMYNQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPESCQHLIHSLNHIKEIP SEG PRD hhhhhhhhhhhcceeeeccchhhhhhhhhhhhhcccceeeeecchhhhhhhhhccccccc SEQ GIGYKTAKCLEALGINSVRDLQTFSPKILEKELGISVAQRIQKLSFGEDNSPVILSGPPQ SEG PRD ccchhhhhhhhhhccccchhhhhhhhhhhhhhccchhhhhhhhhhcccccceeeeccccc SEQ SFSEEDSFKKCTΞEVEAKNKIEELLASLLNRVCQDGRKPHTVRLIIRRYSSEKHYGRESR SEG PRD ccccccccccchhhhhhhhhhhhhhhhhhhhhhhccccccceeeehhhhhhhhhhhcccc SEQ QCPIPSHVIQKLGTGNYDVMTPMVDILMKLFRNMVNVKMPFHLTLLSVCFCNLKALNTAK SEG PRD ccccccceeeeccccccccchhhhhhhhhhhhhhhhhcccceeeeeeeeechhhhhhhhh SEQ KGLIDYYLMPSLSTTSRSGKHSFKMKDTHMEDFPKDKETNRDFLPΞGRIESTRTRESPLD SEG PRD hhhheeeecccccccccccccceeeccccccccccccccccccccccccccccccccccc SEQ TTNFSKEKDINEFPLCSLPEGVDQEVSKQLPVDIQEEILSGKSREKFQGKGSVΞCPLHAS SEG PRD cccccccccccccccccccchhhhhhhhhhhhhhhhhhhcccceeeeecccccccchhhh SEQ RGVLSFFSKKQMQDIPINPRDHLSSSKQVSSVSPCEPGTSGFNSSSSSYMSSQKDYSYYL SEG xxxxxxxxxx xxxxxxxxxxxxxxxxxxxx . PRD hcccccccccccccccccccccccccccccccccccccccccccccccccccccchhhhh SEQ DNRLKDERIΞQGPKEPQGFHFTNSNPAVSAFHSFPNLQSEQLFSRNHTTDΞHKQTVATDS SEG PRD hhhhhhhhhhcccccccceeeeccccceeecccccccchhhhhhhccccccceeeeeecc SEQ HEGLTENREPDSVDEKITFPSDIDPQVFYELPEAVQKELLAEWKRTGSDFHIGHK SEG PRD ccccccccccccccccccccccccceeehhhhhhhhhhhhhhhhhcccccccccc w w w cQ cn ω cn w cn w cn tn cn ω tn cn co ω cn tΛ w w cn w cn cn cn w w o o OoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoooooooooo o OoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOoOo^ooooooooo OOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOO'O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O hh-- » >000000000000OOOOO0OOOOOOO0OOOO<O 000 O 0 O O 0 O O O 0 O O O O 0 O O O 00 O O 000 O 0 O O O O O ' — — _ iii^? o∞o ccoo oαDD ccoo o∞o cαoϊ -^-j ~^-4 θo <_rι crt ccτrιt σσt cmrι ιc^ cΛ σ C^ π lJi l.π Λ Λ μ μ μ μ DKFZphfbr2_72dl3 group: brain derived DKFZphfbr2_72dl3 encodes a novel 165 amino acid protein without similarity to known proteins. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of brain-specific genes . unknown seems to be testis specific 9 of 10 EST hits are from testis l brarys Sequenced by LMU Locus : unknown Insert length: 723 bp Poly A stretch at pos. 704, no polyadenylation signal found 1 AGGGGGGGTA TGGGGGAGGG GGAGACTCTG CAGGAGCCTA ATTCCCCACT 51 CTGAGCTCAC CCTTCTGTCT GCCCGGGCCC TACCCCTTCC CCTACTCTCA 101 CCCTTATAAT CCTTTTCAGC ACTAGGTCTT CCCGTCACCT CCACCTCTCT 151 CCATGACCCG GCTCTGCTTA CCCAGACCCG AAGCACGTGA GGATCCGATC 201 CCAGTTCCTC CAAGGGGCCT GGGTGCTGGG GAGGGGTCAG GTAGTCCAGT 251 GCGTCCACCT GTATCCACCT GGGGCCCTAG CTGGGCCCAG CTCCTGGACA 301 GTGTCCTATG GCTGGGGGCA CTAGGACTGA CAATCCAGGC AGTCTTTTCC 351 ACCACTGGCC CAGCCCTGCT GCTGCTTCTG GTCAGCTTCC TCACCTTTGA 401 CCTGCTCCAT AGGCCCGCAG GTCACACTCT GCCACAGCGC AAACTTCTCA 451 CCAGGGGCCA GAGTCAGGGG GCCGGTGAAG GTCCTGGACA GCAGGAGGCT 501 CTACTCCTGC AAATGGGTAC AGTCTCAGGA CAACTTAGCC TCCAGGACGC 551 ACTGCTGCTG CTGCTCATGG GGCTGGGCCC GCTCCTGAGA GCCTGTGGCA 601 TGCCCTTGAC CCTGCTTGGC CTGGCTTTCT GCCTCCATCC TTGGGCCTGA 651 GAGCCCCTCC CCACAACTCA GTGTCCTTCA AATATACAAT GACCACCCTT 701 CTTCAAAAAA AAAAAAAAAA AAC BLAST Results Entry HS860F19 from database EMBLNEW: Human DNA sequence *** SEQUENCING IN PROGRESS *** from clone 860F19 Score = 2059, P = l.le-85, identities = 423/434 2 exons Medline entries No Medline entry Peptide information for frame 3 ORF from 153 bp to 647 bp; peptide length: 165 Category: putative protein Classification: no clue 1 MTRLCLPRPE AREDPIPVPP RGLGAGEGSG SPVRPPVSTW GPSWAQLLDS 51 VLWLGALGLT IQAVFSTTGP ALLLLLVSFL TFDLLHRPAG HTLPQRKLLT 101 RGQSQGAGEG PGQQEALLLQ MGTVSGQLSL QDALLLLLMG LGPLLRACGM 151 PLTLLGLAFC LHPWA BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_72dl3, frame 3 No Alert BLASTP hits found Pedant information for DKFZphfbr2_72dl3 , frame 3 Report for DKFZphfbr2_72dl3 . 3 [ LENGTH ] 165 [MW ] 17393 . 73 [pi ] 7 . 80 [BLOCKS] BL00068A Malate dehydrogenase proteins [KW] TRANSMEMBRANE 2 [KW] LOW COMPLEXITY 29.70 % SEQ MTRLCLPRPEAREDPIPVPPRGLGAGEGSGSPVRPPVSTWGPSWAQLLDSVLWLGALGLT SEG PRD ccccccccccccccccccccccccccccccccccccccccccchhhhhhhhhhhcccccc MEM SEQ IQAVFSTTGPALLLLLVSFLTFDLLHRPAGHTLPQRKLLTRGQSQGAGEGPGQQEALLLQ SEG xxxxxxxxxxxxxx xxxxxxxxxxxxxxx .... PRD eeeecccccchhhhhhhhhhhhhhccccccccccccccccccccccccccccchhhhhhh MEM MMMMMMMMMMMMMMMMM SEQ MGTVSGQLSLQDALLLLLMGLGPLLRACGMPLTLLGLAFCLHPWA SEG xxxxxxxxxxxxxxxxxxxx PRD hcccccchhhhhhhhhhhhccchhhhhcccccchhhhhhhccccc MEM MMMMMMMMMMMMMMMMM (No Prosite data available for DKFZphfbr2_72dl3.3) (No Pfam data available for DKFZphfbr2 72dl3.3) DKFZphfbr2_72112 group: nucleic acid management Summary DKFZphfbr2_72112 encodes a novel 344 amino acid protein with similarity to YDR126w and other S. cerevisiae proteins. The novel protein contains a myc-type, helix-loop-helix dimerization domain signature. This helix-loop-helix domain mediates protein dimerization and has been found in proteins such as the myc family of cellular oncogenes, proteins involved in myogenesis and vertebrate proteins that bind specific DNA sequences in various lmmunoglobulin chains enhancers. Therefore, the protein could be a novel DNA-bindmg protein. The new protein can application in modulating gene expression. similarity to YDR126w ; membrane regions: 2 similarity to YDR126w complete cDNA complete eds, EST hits Sequenced by LMU Locus: unknown Insert length: 1270 bp Poly A stretch at pos. 1251, no polyadenylation signal found 1 GGGGGCGCCC GGGAGGCGCC GGAGCCCAGC GGCTGGCGCC AGATCCAGGC 51 TCCTGGAAGA ACCATGTCCG GCAGCTACTG GTCATGCCAG GCACACACTG 101 CTGCCCAAGA GGAGCTGCTG TTTGAATTAT CTGTGAATGT TGGGAAGAGG 151 AATGCCAGAG CTGCCGGCTG AAAATTACCC AACCAAGAGA AATCTGCAGG 201 ATGGACTTTC TGGTCCTCTT CTTGTTCTAC CTGGCTTCGG TGCTGATGGG 251 TCTTGTTCTT ATCTGCGTCT GCTCGAAAAC CCATAGCTTG AAAGGCCTGG 301 CCAGGGGAGG AGCACAGATA TTTTCCTGTA TAATTCCAGA ATGTCTTCAG 351 AGAGCCGTGC ATGGATTGCT TCATTACCTT TTCCATACGA GAAACCACAC 401 CTTCATTGTC CTGCACCTGG TCTTGCAAGG GATGGTTTAT ACTGAGTACA 451 CCTGGGAAGT ATTTGGCTAC TGTCAGGAGC TGGAGTTGTC CTTGCATTAC 501 CTTCTTCTGC CCTATCTGCT GCTAGGTGTA AACCTGTTTT TTTTCACCCT 551 GACTTGTGGA ACCAATCCTG GCATTATAAC AAAAGCAAAT GAATTATTAT 601 TTCTTCATGT TTATGAATTT GATGAAGTGA TGTTTCCAAA GAACGTGAGG 651 TGCTCTACTT GTGATTTAAG GAAACCAGCT CGATCCAAGC ACTGCAGTGT 701 GTGTAACTGG TGTGTGCACC GTTTCGACCA TCACTGTGTT TGGGTGAACA 751 ACTGCATCGG GGCCTGGAAC ATCAGGTACT TCCTCATCTA CGTCTTGACC 801 TTGACGGCCT CGGCTGCCAC CGTCGCCATT GTGAGCACCA CTTTTCTGGT 851 CCACTTGGTG GTGATGTCAG ATTTATACCA GGAGACTTAC ATCGATGACC 901 TTGGACACCT CCATGTTATG GACACGGTCA TTCTTATTCA GTACCTGTTC 951 CTGACTTTTC CACGGATTGT CTTCATGCTG GGCTTTGTCG TGGTCCTGAG 1001 CTTCCTCCTG GGTGGCTACC TGTTGTCTGT CCTGTATCTG GCGGCCACCA 1051 ACCAGACTAC TAACGAGTGG TACAGAGGTG TCTGGGCCTG GTGCCAGCGT 1101 TGTCCCCTTG TGGCCTGGCC TCCGTCAGCA GAGCCCCAAG TCCACCGGAA 1151 CATTCACTCC CATGGGCTTC GGAGCAACCT TCAAGAGATC TTTCTACCTG 1201 CCTTTCCATG TCATGAGAGG AAGAAACAAG AATGACAAGT GTATGACTGC 1251 CAAAAAAAAA AAAAAAAAAC BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 3 ORF from 201 bp to 1232 bp; peptide length: 344 Category: similarity to unknown protein 1 MDFLVLFLFY LASVLMGLVL ICVCSKTHSL KGLARGGAQI FSCIIPECLQ 51 RAVHGLLHYL FHTRNHTFIV LHLVLQGMVY TEYTWEVFGY CQELELSLHY 101 LLLPYLLLGV NLFFFTLTCG TNPGIITKAN ELLFLHVYEF DEVMFPKNVR 151 CSTCDLRKPA RSKHCSVCNW CVHRFDHHCV WVNNCIGAWN IRYFLIYVLT 201 LTASAATVAI VSTTFLVHLV VMSDLYQETY IDDLGHLHVM DTVILIQYLF 251 LTFPRIVFML GFVVVLSFLL GGYLLSVLYL AATNQTTNEW YRGVWAWCQR 301 CPLVAWPPSA EPQVHRNIHS HGLRSNLQEI FLPAFPCHER KKQE BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_72112, frame 3 TREMBL :SPBC13G1_7 gene: "SPBC13G1.07"; product: "hypothetical protein"; S. pombe chromosome II cosmid cl3Gl., N = 2, Score = 247, P = 1.4e-22 TREMBL:CED2021_3 gene: "D2021.2"; Caenorhabditis elegans cosmid D2021., N = 1, Score = 209, P = 9e-17 TREMBL :CEC43H6_2 gene: "C43H6.7"; Caenorhabditis elegans cosmid C43H6., N = 1, Score = 206, P = 5.2e-15 PIR:S52691 probable membrane protein YDR126w - yeast (Saccharomyces cerevisiae), N = 1, Score = 207, P = 8.4e-15 PIR:E71607 metal binding protein (DHHC domain) PFB0725C - malaria parasite (Plasmodium falciparum), N = 1, Score = 182, P = l.le-13 >TREMBL:SPBC13G1_7 gene: "SPBC13G1.07"; product: "hypothetical protein"; S. pombe chromosome II cosmid cl3Gl. Length = 356 HSPs: Score = 247 (37.1 bits), Expect = 1.4e-22, Sum P(2) = 1.4e-22 Identities = 55/148 (37%), Positives = 85/148 (57%) Query: 52 AVHGLLHYLFHTRNH--TFIVLHLVLQGM VYTEYTWEVFGYCQELELSLHYLLLPY 105 A+ L +Y+ + N F+ L L+ G+ +Y + F + + L +LLPY Sbjct: 64 AMRSLSNYVLYKNNPLVVFLYLALITIGIASFFIYGSΞLTQKFSIIDWISV-LTSVLLPY 122 Query: 106 LLLGVNLFFFTLTCGTNPGIITKANELLFLHVYEFD-EVMFPKNVRCSTCDLRKPARSKH 164 ++L+ + +NPG I N + +D ++ FP +CSTC KPARSKH Sbjct: 123 ISLY IAAKSNPGKIDLKNWNEASRRFPYDYKIFFPN—KCSTCKFEKPARSKH 173 Query: 165 CSVCNWCVHRFDHHCVWVNNCIGAWNIRYFLIYVL 199 C +CN CV +FDHHC+W+NNC+G N RYF +++L Sbjct: 174 CRLCNICVEKFDHHCIWINNCVGLNNARYFFLFLL 208 Score = 43 (6.5 bits), Expect = 1.4e-22, Sum P(2) = 1.4e-22 Identities = 10/35 (28%), Positives = 17/35 (48%) Query: 257 VFMLGFVV-VLSFLLGGYLLSVLYLAATNQTTNEW 290 VF++ + VL L GY ++Y T + +W Sbjct: 254 VFLISLICSVLVLCLLGYEFFLVYAGYTTNESEKW 288 Pedant information for DKFZphfbr2_72112, frame 3 Report for DKFZphfbr2_72112.3 [LENGTH] 344 [MW] 39677.23 [pi] 7.26 [HOMOL] TREMBL :SPBC13G1_7 gene: "SPBC13G1.07"; product: "hypothetical protein"; S. pombe chromosome II cosmid C13G1. 3e-17 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YDR126w] le-16 [FUNCAT] 03.07 pheromone response, mating-type determination, sex-specific proteins [S. cerevisiae, YDR264c] 8e-05 [FUNCAT] 10.05.99 other pheromone response activities [S. cerevisiae, YDR264C] 8e-05 [PIRKW] transmembrane protein 4e-15 [SUPFAM] ankyrin repeat homology le-10 [SUPFAM] unassigned ankyrin repeat proteins le-10 [PROSITE] MYRISTYL 4 [PROSITE] CK2_PHOSPHO_SITE 3 [PROSITE] PKC_PHOSPHO_SITE 1 [PROSITE] ASN_GLYCOSYLATION 2 [KW] SIGNAL_PEPTIDE 30 [KW] TRANSMEMBRANE 2 [KW] LOW COMPLEXITY 16.57 SEQ MDFLVLFLFYLASVLMGLVLICVCSKTHSLKGLARGGAQIFSCIIPECLQRAVHGLLHYL SEG PRD ccchhhhhhhhhhhhhhheeeeeeccccceeeeecccceeeeeeehhhhhhhhhhhheee MEM SEQ FHTRNHTFIVLHLVLQGMVYTEYTWEVFGYCQELELSLHYLLLPYLLLGVNLFFFTLTCG SEG xxxxxxxxxxxxxxxxxxx PRD ecccchhhhhhhhhhccchhhhhhhheeeeccceeehhhhhhhhhhhhhhcccceeeecc MEM MMMMMMMMMMMMMMMMMMMMMMMMM SEQ TNPGIITKANELLFLHVYEFDEVMFPKNVRCSTCDLRKPARSKHCSVCNWCVHRFDHHCV SEG PRD ccccccccccchhhhhhhhhcccccccceeeecccccccccccccccceeeecccccccc MEM M MMMMMMMMMMMMMMMMMMMMMMMMMMMM SEQ WVNNCIGAWNIRYFLIYVLTLTASAATVAIVSTTFLVHLVVMSDLYQETYIDDLGHLHVM SEG xxxxxxxxxxxxxxxxx PRD cccccccccccchhhhhhhhhccchhhhhhhhhhhhhhhhhccccccccccccccccchh MEM SEQ DTVILIQYLFLTFPRIVFMLGFVVVLΞFLLGGYLLSVLYLAATNQTTNEWYRGVWAWCQR SEG xxxxxxxxxxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhccccccccceeecccchhhhhhhhhcccchhhhhhhhhhhcccc MEM SEQ CPLVAWPPSAEPQVHRNIHSHGLRSNLQEIFLPAFPCHERKKQE SEG PRD cccccccccccccceeecccccccccceeeeecccccccccccc MEM Prosite for DKFZphfbr2_72112.3 PS00001 65->69 ASN_GLYCOSYLATION PDOC00001 PS00001 284->288 ASN_GLYCOSYLATION PDOC00001 PS00005 29->32 PKC_PHOSPHO_SITE PDOC00005 PS00006 152->156 CK2_PHOSPHO_SITE PDOC00006 PS00006 229->233 CK2_PHOSPHO_SITE PDOC00006 PS00006 286->290 CK2_PHOSPHO_SI E PDOC00006 PS00008 32->38 MYRISTYL PDOC00008 PS00008 77->83 MYRISTYL PDOC00008 PS00008 120->126 MYRISTYL PDOC00008 PS00008 322->328 MYRISTYL PDOC00008 (No Pfam data available for DKFZphfbr2_72112.3) DKFZphfbr2_72ml6 group: unknown DKFZphfbr2_72ml6 encodes a novel 287 am o acid protein without similarity to known proteins. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of brain-specific genes . unknown complete cDNA, complete eds, EST hits Sequenced by LMU Locus: /map="26.2 cR from top of Chrl6 linkage group" Insert length: 1462 bp Poly A stretch at pos. 1441, polyadenylation signal at pos. 1421 1 GGGGAGGACC GGAGGACCGA GGACAGAAAG ATTGGTGGAC AGGAGCAGCG 51 GCCGGTGGGG AGGGCGCTCG GCGGCGGCCT GCGGCCATGG CCACCGTGAT 101 GGCAGCGACG GCGGCGGAGC GGGCGGTGCT GGAGGAGGAG TTCCGCTGGC 151 TGCTGCACGA CGAGGTGCAC GCTGTGTTGA AGCAGCTGCA GGACATCCTC 201 AAGGAGGCCT CTCTGCGCTT CACTCTGCCG GGCTCCGGCA CTGAGGGGCC 251 CGCCAAGCAA GAGAACTTCA TCCTAGGCAG CTGTGGCACA GACCAGGTGA 301 AGGGTGTGCT GACTCTGCAG GGGGATGCCC TCAGCCAGGC GGATGTGAAC 351 CTGAAGATGC CCCGGAACAA CCAGCTGCTG CACTTCGCCT TCCGGGAGGA 401 CAAGCAGTGG AAGCTGCAGC AGATCCAGGA TGCCAGAAAC CATGTGAGCC 451 AAGCCATTTA CCTGCTTACC AGCCGGGACC AGAGCTACCA GTTCAAGACG 501 GGCGCTGAGG TCCTCAAGCT GATGGACGCA GTGATGCTGC AGCTGACCAG 551 AGCCCGAAAC CGGCTCACCA CCCCCGCCAC CCTCACCCTC CCCGAGATCG 601 CCGCCAGCGG CCTCACGCGG ATGTTCGCCC CTGCCCTGCC GTCCGACCTG 651 CTGGTCAACG TCTACATCAA CCTCAACAAG CTCTGCCTCA CGGTGTACCA 701 GCTGCATGCC CTGCAGCCCA ACTCCACCAA GAACTTCCGC CCAGCTGGGG 751 GCGCGGTGCT GCATAGCCCT GGGGCCATGT TCGAGTGGGG CTCTCAGCGC 801 CTGGAGGTGA GCCACGTGCA CAAAGTGGAG TGCGTGATCC CCTGGCTCAA 851 CGACGCCCTG GTCTACTTCA CCGTCTCCCT GCAGCTCTGC CAGCAGCTTA 901 AGGACAAGAT CTCCGTGTTC TCCAGCTACT GGAGCTACAG ACCCTTCTGA 951 TCACAGCACC CAGGAGCTTG TCTCCAGGAA GGCGGCCCCG TCCCCTACTC 1001 ATACCCACCA CAGAGCACCA GCCAGTGCCA ACGCCAGGCT GCTATTTATC 1051 TCCCTATCCC ACCCCCTACC CCACCTAACA CATTTGCACT GCCGGGAATG 1101 GACACTGGAA GTGCCAGGAG GAAGGAAGGC TGGTTTGGTG GGGTAGTGGG 1151 GAGGTCAGGG AGGCGGGGCC AAGGGTGTCC CACATTCCCA ACACCGCCCT 1201 CTGATCACCA TGGGAATCTT TGGACTCAGG ACAGGGCCAG GCGCAGGGCT 1251 CTCCCTCCTC TCCCCTTCGC TGTCCCCTCC CCCTGGAGGG CATGGTGTCG 1301 GGGGGTGGCA CTGAGCTATG AGTCCCGGGG ATGGTGAGGA ACGCCACAGA 1351 CAGAGCCACC CTAGGAGTGA GTATAGTGCT GGTGACTGTG TTTCATAGCC 1401 CCAGTCCAGG GCTGTCTAAG AAATAAAGAT CATCAGACTC CAAAAAAAAA 1451 AAAAAAAAAA AC BLAST Results Entry HS604351 from database EMBL: human STS WI-18474. Score = 1178, P = 1.5e-48, identities = 250/268 Medline entries No Medlme entry Peptide information for frame 3 ORF from 87 bp to 947 bp; peptide length: 287 Category: similarity to unknown protein 1 MATVMAATAA ERAVLEEEFR WLLHDEVHAV LKQLQDILKE ASLRFTLPGS 51 GTEGPAKQEN FILGSCGTDQ VKGVLTLQGD ALSQADVNLK MPRNNQLLHF 101 AFREDKQWKL QQIQDARNHV SQAIYLLTSR DQSYQFKTGA EVLKLMDAVM 151 LQLTRARNRL TTPATLTLPE IAASGLTRMF APALPSDLLV NVYINLNKLC 201 LTVYQLHALQ PNSTKNFRPA GGAVLHSPGA MFEWGSQRLE VSHVHKVECV 251 IPWLNDALVY FTVSLQLCQQ LKDKISVFSS YWSYRPF BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_72ml6, frame 3 No Alert BLASTP hits found Pedant information for DKFZphfbr2_72ml6, frame 3 Report for DKFZphfbr2_72ml6.3 [LENGTH] 287 [MW] 32254.40 [pi] 8.30 [HOMOL] TREMBL:AF025459_2 gene: "H14A12.3"; Caenorhabditis elegans cosmid H14A12. 3e-14 [PROSITE] MYRISTYL 1 [PROSITE] CK2_PHOSPHO_SITE 6 [PROSITE] PKC_PHOSPHO_SI E 5 [PROSITE] ASN_GLYCOSYLATION 1 [KW] Alpha_Beta [KW] LOW COMPLEXITY 6.27 ' SEQ MATVMAATAAERAVLEEEFRWLLHDEVHAVLKQLQDILKEASLRFTLPGSGTEGPAKQEN SEG xxxxxxxxxxxxxxxxxx PRD ccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccccccccccchhhh SEQ FILGSCGTDQVKGVLTLQGDALSQADVNLKMPRNNQLLHFAFREDKQWKLQQIQDARNHV SEG PRD hhccccccceeeeeeeeccccchhhhhhhcccccchhhhhhhhhchhhhhhhhhhhhchh SEQ SQAIYLLTSRDQSYQFKTGAEVLKLMDAVMLQLTRARNRLTTPATLTLPEIAASGLTRMF SEG PRD hhhhhhhhccccceeecchhhhhhhhhhhhhhhhhhhhhhcccccccccccccccccccc SEQ APALPSDLLVNVYINLNKLCLTVYQLHALQPNSTKNFRPAGGAVLHSPGAMFEWGSQRLE SEG PRD cccccccceeeeehhhhhhhhhhheeeecccccccccccccceeecccccccccccccee SEQ VSHVHKVECVIPWLNDALVYFTVSLQLCQQLKDKISVFSSYWSYRPF SEG PRD eeeeeeeeeeeecccceeeeeeehhhhhhhhhhhhheeeeeeeeccc Prosite for DKFZphfbr2_72ml6.3 PS00001 212->216 ASN_GLYCOSYLATION PDOC00001 PS00005 42->45 PKC_PHOSPHO_SITE PDOC00005 PS00005 128->131 PKC_PHOSPHO_SITE PDOC00005 PS00005 213->216 PKC_PHOSPHO_SITE PDOC00005 PS00005 236->239 PKC_PHOSPHO_SITE PDOC00005 PS00005 283->286 PKC_PHOSPHO_SITE PDOC00005 PS00006 8->12 CK2_PHOSPHO_SITE PDOC00006 PS00006 50->54 CK2_PHOSPHO_SITE PDOC00006 PS00006 83->87 CK2_PHOΞPHO_SITE PDOC00006 PS00006 128->132 CK2_PHOSPHO_SITE PDOC00006 PS00006 138->142 CK2_PHOSPHO_SITE PDOC00006 PS00006 167-M71 CK2_PHOSPHO_SITE PDOC00006 PS00008 64->70 MYRISTYL PDOC00008 (No Pfam data available for DKFZphfbr2_72ml6.3) DKFZphfbr2_72nl2 group: brain derived DKFZphfbr2_72nl2 encodes a novel 117 amino acid protein with similarity to a protein with conserved sequence in bacteria and eukaπota. The novel protein is very similar to human MM46, human and rat gangliosiode expression factor- 2 (GEF2), C. elegans 14.8 kD protein C32D5.9 and Laccaria bicolor symbiosis-related protein LBU93506_1. The function of this highly conserved proteins is not known. The new protein can find application in studying the expression profile of brain-specific genes . strong similarity to rat GANGLIOSIDE EXPRESSION FACTOR 2 (GEF-2) complete cDNA, complete eds, EST hits Sequenced by LMU Locus: /map="12" Insert length: 1880 bp Poly A stretch at pos. 1859, polyadenylation signal at pos. 1830 1 GGGGGCCGGT ATTTCTCCAT CTGGCTCTCC TCTACCTCCA GGCAGGCTCA 51 CCCGAGATCC CCGCCCCGAA CCCCCCCTGC ACACTCGGCC CAGCGCTGTT 101 GCCCCCGGAG CGGACGTTTC TGCAGCTATT CTGAGCACAC CTTGACGTCG 151 GCTGAGGGAG CGGGACAGGG TCAGCGGCGA AGGAGGCAGG CCCCGCGCGG 201 GGATCTCGGA AGCCCTGCGG TGCATCATGA AGTTCCAGTA CAAGGAGGAC 251 CATCCCTTTG AGTATCGGAA AAAGGAAGGA GAAAAGATCC GGAAGAAATA 301 TCCGGACAGG GTCCCCGTGA TTGTAGAGAA GGCTCCAAAA GCCAGGGTGC 351 CTGATCTGGA CAAGAGGAAG TACCTAGTGC CCTCTGACCT TACTGTTGGC 401 CAGTTCTACT TCTTAATCCG GAAGAGAATC CACCTGAGAC CTGAGGACGC 451 CTTATTCTTC TTTGTCAACA ACACCATCCC TCCCACCAGT GCTACCATGG 501 GCCAACTGTA TGAGGACAAT CATGAGGAAG ACTATTTTCT GTATGTGGCC 551 TACAGTGATG AGAGTGTCTA TGGGAAATGA GTGGTTGGAA GCCCAGCAGA 601 TGGGAGCACC TGGACTTGGG GGTAGGGGAG GGGTGTGTGT GCGCGACATG 651 GGGAAAGAGG GTGGCTCCCA CCGCAAGGAG ACAGAAGGTG AAGACATCTA 701 GAAACATTAC ACCACACACA CCGTCATCAC ATTTTCACAT GCTCAATTGA 751 TATTTTTTGC TGCTTCCTCG GCCCAGGGAG AAAGCATGTC AGGACAGAGC 801 TGTTGGATTG GCTTTGATAG AGGAATGGGG ATGATGTAAG TTTACAGTAT 851 TCCTGGGGTT TAATTGTTGT GCAGTTTCAT AGATGGGTCA GGAGGTGGAC 901 AAGTTGGGGC CAGAGATGAT GGCAGTCCAG CAGCAACTCC CTGTGCTCCC 951 TTCTCTTTGG GCAGAGATTC TATTTTTGAC ATTTGCACAA GACAGGTAGG 1001 GAAAGGGGAC TTGTGGTAGT GGACCATACC TGGGGACCAA AAGAGACCCA 1051 CTGTAATTGA TGCATTGTGG CCCCTGATCT TCCCTGTCTC ACACTTCTTT 1101 TCTCCCATCC CGGTTGCAAT CTCACTCAGA CATCACAGTA CCACCCCAGG 1151 GGTGGCAGTA GACAACAACC CAGAAATTTA GACAGGGATC TCTTACCTTT 1201 GGAAAATAGG GGTTAGGCAT GAAGGTGGTT GTGATTAAGA AGATGGTTTT 1251 GTTATTAAAT AGCATTAAAC TGGAATTGAC AAGAGTGTTG AGCATCCCTG 1301 TCTAACCTGC TCTTTCTCTT TGGTGCCCCT TATCTCACCC CTTCCTTGGA 1351 ATTTAATAAG TCTCAGGCAT TTCCAATTGT AGACTAAAAC CACTCTTAGC 1401 ATCTCCTCTA GTATTTTCCA TGTATCAGGA AAGAGGTGTC TTATGTAGGG 1451 AGGGGGCAAG TATGAAGTAA GGTAATTATA TACTACTCTC ATTCAGGATT 1501 CTTGCTCCCA TGCTGCTGTC CCTTCAGGCT CACATGCACA GGAATGCTAC 1551 ATGATGGCCA GCTGCTTCCC TCCTTGGTTA TCATCCACTG CAGCTGCTAG 1601 TTAGAAAGGT TTGGAGGGAT GACTTTTAGT AAATCATGGG GATTTTATTG 1651 ATTTATTTTC ACTTTTGGGA TTTTGTGGGG TGGGAGTGGG GAGCAGGAAT 1701 TGCACTCAGA CATGACATTT CAATTCATCT CTGCTAATGA AAAGGGTTCT 1751 TTCTCTTGGG GGAAATGTGT GTGTCAGTTC TGTCAGCTGC AAGTTCTTGT 1801 ATAATGAAGT CAATGCCATC AGGCCAAGGA AATAAAATAA TTGCTTACCT 1851 TAAAAATCGA AAAAAAAAAA AAAAAAAAAC BLAST Results Entry HS418210 from database EMBL: human STS SHGC-10496. Score = 1916, P = 4.0e-80, identities = 394/400 Entry AC006514 from database EMBLNEW: *** SEQUENCING IN PROGRESS *** Homo sapiens; HTGS phase 1, 68 unordered pieces . Score = 610 , P = 2 .7e-16, identities = 128 /134 4 exons Medl e entries No Medlme entry Peptide information for frame 2 ORF from 227 bp to 577 bp; peptide length: 117 Category: strong similarity to known protein 1 MKFQYKEDHP FEYRKKEGEK IRKKYPDRVP VIVEKAPKAR VPDLDKRKYL 51 VPSDLTVGQF YFLIRKRIHL RPEDALFFFV NNTIPPTSAT MGQLYEDNHE 101 EDYFLYVAYS DESVYGK BLASTP hits Entry YQD9_CAEEL from database SWISSPROT: HYPOTHETICAL 14.8 KD PROTEIN C32D5.9 IN CHROMOSOME II. Score = 496, P = 1.8e-47, identities = 91/116, positives = 105/116 Entry SYRP_LACBI from database SWISSPROT: SYMBIOSIS-RELATED PROTEIN. Score = 390, P = 3.1e-36, identities = 68/117, positives = 94/117 Entry LBU93506_1 from database TREMBL: product: "symbiosis-related protein"; Laccaria bicolor symbiosis-related protein mRNA, partial eds. Score = 390, P = 3.1e-36, identities = 68/117, positives = 94/117 Entry GEF2_RAT from database SWISSPROT: GANGLIOSIDE EXPRESSION FACTOR 2 (GEF-2) . Score = 373, P = 2.0e-34, identities = 71/116, positives = 88/116 Alert BLASTP hits for DKFZphfbr2_72nl2, frame 2 TREMBLNEW:AF044671_1 product: "MM46"; Homo sapiens MM46 mRNA, complete eds., N = 1, Score = 549, P = 4.7e-53 SWISSPROT:GEF2_HUMAN GANGLIOSIDE EXPRESSION FACTOR 2 (GEF-2)., N = 1, Score = 373, P = 2.1e-34 >TREMBLNEW:AF044671_1 product: "MM46"; Homo sapiens MM46 mRNA, complete eds. Length = 117 HSPs: Score = 549 (82.4 bits), Expect = 4.7e-53, P = 4.7e-53 Identities = 101/116 (87%), Positives = 110/116 (94%) Query: 1 MKFQYKEDHPFEYRKKEGEKIRKKYPDRVPVIVEKAPKARVPDLDKRKYLVPSDLTVGQF 60 MKF YKE+HPFE R+ EGEKIRKKYPDRVPVIVEKAPKAR+ DLDK+KYLVPSDLTVGQF Sbjct: 1 MKFVYKEEHPFEKRRSEGEKIRKKYPDRVPVIVEKAPKARIGDLDKKKYLVPSDLTVGQF 60 Query: 61 YFLIRKRIHLRPEDALFFFVNNTIPPTSATMGQLYEDNHEEDYFLYVAYSDEΞVYG 116 YFLIRKRIHLR EDALFFFVNN IPPTSATMGQLY+++HEED+FLY+AYSDESVYG Sbjct: 61 YFLIRKRIHLRAEDALFFFVNNVIPPTSATMGQLYQEHHEEDFFLYIAYSDESVYG 116 Pedant information for DKFZphfbr2_72nl2, frame 2 Report for DKFZphfbr2_72nl2.2 [LENGTH] 117 [MW] 14044.07 [pi] 8.67 [HOMOL] TREMBL:AF044671_1 product: "MM46"; Homo sapiens MM46 mRNA, complete eds. le-56 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YBL078c] 4e-36 [FUNCAT] 08.22 cytoskeleton-dependent transport [S. cerevisiae, YBL078c] 4e-36 [FUNCAT] 06.13.04 lysosomal and vacuolar degradation [S. cerevisiae, YBL078c] 4e-36 [SUPFAM] hypothetical protein YBL078C 8e-35 [PROSITE] ASN_GLYCOSYLATION 1 [KW] Alpha_Beta SEQ MKFQYKEDHPFEYRKKEGEKIRKKYPDRVPVIVEKAPKARVPDLDKRKYLVPSDLTVGQF PRD cccccccccchhhhhhhhhhhhhhccccceeeeeccccccccccccceeecccccchhhh SEQ YFLIRKRIHLRPEDALFFFVNNTIPPTSATMGQLYEDNHEEDYFLYVAYSDESVYGK PRD hhhhhhhhhhccccceeeeecccccccchhhhhhhhhccccceeeeeeecccccccc Prosite for DKFZphfbr2_72nl2.2 PS00001 81->85 ASN_GLYCOSYLATION PDOC00001 (No Pfam data available for DKFZphfbr2_72nl2.2) DKFZphfbr2_78c24 group: signal transduction DKFZphfbr2_78c24 encodes a novel 563 ammo acid protein with strong similarity to guanylate- bindmg proteins (GBPs) . GBPs were originally described as proteins that are strongly induced by mterferons and are capable of binding to agarose-immobilized guanine nucleotides. hGBPl, the first of two members of this protein family in humans, represents a novel type of GTPase. The novel protein contains an ATP/GTP-binding site motif A (P-loop) and a RGD cell attachment site. It seems to be a new member of the GBP-family and shows a splicing pattern not described previously. The new protein can find application in modulating/blocking the response of cells to mterferons . strong similarity to guanine nucleotide-binding protein 1/2 but different "splice variant" aa 211-245 of GBP1/2 missing Sequenced by MediGenomix Locus: unknown Insert length: 2952 bp Poly A stretch at pos. 2927, polyadenylation signal at pos. 2914 1 CAGTTTCATT AGGCTCTGAA GCCATTACAA AGGTTGCTTA ACTTCTAATT 51 ATTTGATCAC TGAGGAAAAT CCAGAAAGCT ACACAACACT GAAGGGGTGA 101 AATAAAAGTC CAGCGATCCA GCGAAAGAAA AGAGAAGTGA CAGAAACAAC 151 TTTACCTGGA CTGAAGATAA AAGCACAGAC AAGAGAACAA TGCCCTGGAC 201 ATGGCTCCAG AGATCCACAT GACAGGCCCA ATGTGCCTCA TTGAGAACAC 251 TAATGGGGAA CTGGTGGCGA ATCCAGAAGC TCTGAAAATC CTGTCTGCCA 301 TTACACAGCC TGTGGTGGTG GTGGCAATTG TGGGCCTCTA CCGCACAGGA 351 AAATCCTACC TGATGAACAA GCTAGCTGGG AAGAATAAGG GCTTCTCTCT 401 GGGCTCCACA GTGAAATCTC ACACCAAAGG AATCTGGATG TGGTGTGTGC 451 CTCACCCCAA AAAGCCAGAA CACACCTTAG TCCTGCTTGA CACTGAGGGC 501 CTGGGAGATG TAAAGAAGGG TGACAACCAG AATGACTCCT GGATCTTCAC 551 CCTGGCCGTC CTCCTGAGCA GCACTCTCGT GTACAATAGC ATGGGAACCA 601 TCAACCAGCA GGCTATGGAC CAACTGTACT ATGTGACAGA GCTGACACAT 651 CGAATCCGAT CAAAATCCTC ACCTGATGAG AATGAGAATG AGGATTCAGC 701 TGACTTTGTG AGCTTCTTCC CAGATTTTGT GTGGACACTG AGAGATTTCT 751 CCCTGGACTT GGAAGCAGAT GGACAACCCC TCACACCAGA TGAGTACCTG 801 GAGTATTCCC TGAAGCTAAC GCAAGGTAAC AGGAAGCTTG CCCAGCTTGA 851 GAAACTACAA GATGAAGAGC TGGACCCTGA ATTTGTGCAA CAAGTAGCAG 901 ACTTCTGTTC CTACATCTTT AGCAATTCCA AAACTAAAAC TCTTTCAGGA 951 GGCATCAAGG TCAATGGGCC TTGTCTAGAG AGCCTAGTGC TGACCTATAT 1001 CAATGCTATC AGCAGAGGGG ATCTGCCCTG CATGGAGAAC GCAGTCCTGG 1051 CCTTGGCCCA GATAGAGAAC TCAGCCGCAG TGCAAAAGGC TATTGCCCAC 1101 TATGACCAGC AGATGGGCCA GAAGGTGCAG CTGCCCGCAG AAACCCTCCA 1151 GGAGCTGCTG GACCTGCACA GGGTTAGTGA GAGGGAGGCC ACTGAAGTCT 1201 ATATGAAGAA CTCTTTCAAG GATGTGGACC ATCTGTTTCA AAAGAAATTA 1251 GCGGCCCAGC TAGACAAAAA GCGGGATGAC TTTTGTAAAC AGAATCAAGA 1301 AGCATCATCA GATCGTTGCT CAGCTTTACT TCAGGTCATT TTCAGTCCTC 1351 TAGAAGAAGA AGTGAAGGCG GGAATTTATT CGAAACCAGG GGGCTATTGT 1401 CTCTTTATTC AGAAGCTACA AGACCTGGAG AAAAAGTACT ATGAGGAACC 1451 AAGGAAGGGG ATACAGGCTG AAGAGATTCT GCAGACATAC TTGAAATCCA 1501 AGGAGTCTGT GACCGATGCA ATTCTACAGA CAGACCAGAT TCTCACAGAA 1551 AAGGAAAAGG AGATTGAAGT GGAATGTGTA AAAGCTGAAT CTGCACAGGC 1601 TTCAGCAAAA ATGGTGGAGG AAATGCAAAT AAAGTATCAG CAGATGATGG 1651 AAGAGAAAGA GAAGAGTTAT CAAGAACATG TGAAACAATT GACTGAGAAG 1701 ATGGAGAGGG AGAGGGCCCA GTTGCTGGAA GAGCAAGAGA AGACCCTCAC 1751 TAGTAAACTT CAGGAACAGG CCCGAGTACT AAAGGAGAGA TGCCAAGGTG 1801 AAAGTACCCA ACTTCAAAAT GAGATACAAA AGCTACAGAA GACCCTGAAA 1851 AAAAAAACCA AGAGATATAT GTCGCATAAG CTAAAGATCT AAACAACAGA 1901 GCTTTTCTGT CATCCTAACC CAAGGCATAA CTGAAACAAT TTTAGAATTT 1951 GGAACAAGTG TCACTATATT TGATAATAAT TAGATCTTGC ATCATAACAC 2001 TAAAAGTTTA CAAGAACATG CAGTTCAATG ATCAAAATCA TGTTTTTTCC 2051 TTAAAAAGAT TGTAAATTGT GCAACAAAGA TGCATTTACC TCTGTACCAA 2101 CAGAGGAGGG ATCATGAGTT GCCACCACTC AGAAGTTTAT TCTTCCAGAC 2151 GACCAGTGGA TACTGAGGAA AGTCTTAGGT AAAAATCTTG GGACATATTT 2201 GGGCACTGGT TTGGCCAAGT GTACAATAGG TCCCAATATC AGAAACAACC 2251 ATCCTAGCTT CCTAGGGAAG ACAGTGTACA GTTCTCCATT ATATCAAGGC 2301 TACAAGGTCT ATGAGCAATA ATGTGATTTC TGGACATTGC CCATGGATAA 2351 TTCTCACTGA TGGATCTCAA GCTAAAGCAA ACCATCTTAT ACAGAGATCT 2401 AGAATCTTAT ATTTTCCATA GGAAGGTAAA GAAATCATTA GCAAGAGTAG 2451 GAATTGAATC ATAAACAAAT TGGCTAATGA AGAAATCTTT TCTTTCTTGT 2501 TCAATTCATC TAGATTATAA CCTTAATGTG ACACCTGAGA CCTTTAGACA 2551 GTTGACCCTG AATTAAATAG TCACATGGTA ACAATTATGC ACTGTGTAAT 2601 TTTAGTAATG TATAACATGC AATGATGCAC TTTAACTGAA GATAGAGACT 2651 ATGTTAGAAA ATTGAACTAA TTTAATTATT TGATTGTTTT AATCCTAAAG 2701 CATAAGTTAG TCTTTTCCTG ATTCTTAAAG GTCATACTTG AAATCCTGCC 2751 AATTTTCCCC AAAGGGAATA TGGAATTTTT TTTGACTTTC TTTTGAGCAA 2801 TAAAATAATT GTCTTGCCAT TACTTAGTAT ATGTAGACTT CATCCCAATT 2851 GTCAAACATC CTAGGTAAGT GGTTGACATT TCTTACAGCA ATTACAGATT 2901 ATTTTTGAAC TAGAAATAAA CTAAACTAGA AACAAAAAAA AAAAAAAAAA 2951 AA BLAST Results No BLAST result Medline entries No Medlme entry Peptide information for frame 3 ORF from 201 bp to 1889 bp; peptide length: 563 Category: strong similarity to known protein Classification: Cell signaling/communication Prosite motifs: RGD (272-275) ATP GTP A (45-53) 1 MAPEIHMTGP MCLIENTNGE LVANPEALKI LSAITQPVVV VAIVGLYRTG 51 KSYLMNKLAG KNKGFSLGST VKSHTKGIWM WCVPHPKKPE HTLVLLDTEG 101 LGDVKKGDNQ NDSWIFTLAV LLΞSTLVYNS MGTINQQAMD QLYYVTELTH 151 RIRSKSSPDE NENEDΞADFV SFFPDFVWTL RDFSLDLEAD GQPLTPDEYL 201 EYSLKLTQGN RKLAQLEKLQ DEELDPEFVQ QVADFCSYIF SNSKTKTLSG 251 GIKVNGPCLE SLVLTYINAI SRGDLPCMEN AVLALAQIEN SAAVQKAIAH 301 YDQQMGQKVQ LPAETLQELL DLHRVSEREA TEVYMKNSFK DVDHLFQKKL 351 AAQLDKKRDD FCKQNQEASS DRCSALLQVI FSPLEEEVKA GIYSKPGGYC 401 LFIQKLQDLE KKYYEEPRKG IQAEEILQTY LKSKESVTDA ILQTDQILTE 451 KEKEIEVECV KAESAQASAK MVEEMQIKYQ QMMEEKEKSY QEHVKQLTEK 501 MERERAQLLE EQEKTLTSKL QEQARVLKER CQGESTQLQN EIQKLQKTLK 551 KKTKRYMSHK LKI BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_78c24, frame 3 PIR:A41268 guanine nucleotide-bindmg protein 1 - human, N = 2, Score = 1306, P = 4.9e-238 PIR:A46459 macrophage-activation gene-1 protein mag-1 - mouse, N = 2, Score = 942, P = 8.9e-184 PIR:S70524 guanine nucleotide-binding protein 2 - human, N = 2, Score = 1131, P = 4.1e-210 TREMBL :AF077007_1 gene: "Gbp2"; product: "lnterferon-mduced guanylate binding protein GBP-2"; Mus musculus lnterferon-induced guanylate binding protein GBP-2 (Gbp2) mRNA, complete eds., N = 2, Score = 904, P = 1.2e-179 >PIR:A41268 guanine nucleotide-bmdmg protein 1 - human Length = 592 HSPs: Score = 1306 (195.9 bits), Expect = 4.9e-238, Sum P(2) = 4.9e-238 Identities = 264/332 (79%), Positives = 288/332 (86%) Query: 211 RKLAQLEKLQDEELDPEFVQQVADFCSYIFSNSKTKTLSGGIKVNGPCLESLVLTYINAI 270 RKLAQLEKLQDEELDPEFVQQVADFCSYIFSNSKTKTLSGGI+VNGP LESLVLTY+NAI Sbjct: 245 RKLAQLEKLQDEELDPEFVQQVADFCSYIFSNSKTKTLSGGIQVNGPRLESLVLTYVNAI 304 Query: 271 SRGDLPCMENAVLALAQIENSAAVQKAIAHYDQQMGQKVQLPAETLQELLDLHRVSEREA 330 S GDLPCMENAVLALAQIENSAAVQKAIAHY+QQMGQKVQLP E+LQELLDLHR SEREA Sb j ct: 305 SSGDLPCMENAVLALAQIENSAAVQKAIAHYEQQMGQKVQLPTESLQELLDLHRDSEREA 364 Query: 331 TEVYMKNSFKDVDHLFQKKLAAQLDKKRDDFCKQNQEASSDRCSALLQVIFSPLEEEVKA 390 EV++++SFKDVDHLFQK+LAAQL+KKRDDFCKQNQEASSDRCS LLQVIFSPLEEEVKA Sbjct: 365 IEVFIRSSFKDVDHLFQKELAAQLEKKRDDFCKQNQEASSDRCSGLLQVIFSPLEEEVKA 424 Query: 391 GIYΞKPGGYCLFIQKLQDLEKKYYEEPRKGIQAEEILQTYLKSKESVTDAILQTDQILTX 450 GIYSKPGGY LF+QKLQDL+KKYYEEPRKGIQAEEILQTYLKSKES+TDAILQTDQ LT Sbjct: 425 GIYSKPGGYRLFVQKLQDLKKKYYEEPRKGIQAEEILQTYLKSKESMTDAILQTDQTLTE 484 Query: 451 XXXXXXXXXXXXXSAQASAKMVEEMQIKYQQMMEEKEKSYQEHVKQLTEKMXXXXXXXXX 510 SAQASAKM++EMQ K +QMME+KE+SYQEH+KQLTEKM Sbjct: 485 KEKEIEVERVKAESAQASAKMLQEMQRKNEQMMEQKERSYQEHLKQLTEKMENDRVQLLK 544 Query: 511 XXXKTLTSKLQEQARVLKERCQGESTQLQNEI 542 +TL KLQEQ ++LKE Q ES ++NEI Sbjct: 545 EQERTLALKLQEQEQLLKEGFQKESRIMKNEI 576 Score = 1012 (151.8 bits), Expect = 4.9e-238, Sum P(2) 4.9e-238 Identities = 194/211 (91%), Positives = 200/211 (94%) Query: 1 MAPEIHMTGPMCLIENTNGELVANPEALKILSAITQPVVVVAIVGLYRTGKSYLMNKLAG 60 MA EIHMTGPMCLIENTNG L+ANPEALKILSAITQP+VVVAIVGLYRTGKSYLMNKLAG Sbjct: 1 MASEIHMTGPMCLIENTNGRLMANPEALKILSAITQPMVVVAIVGLYRTGKSYLMNKLAG 60 Query: 61 KNKGFSLGSTVKSHTKGIWMWCVPHPKKPEHTLVLLDTEGLGDVKKGDNQNDSWIFTLAV 120 K KGFSLGSTV+SHTKGIWMWCVPHPKKP H LVLLDTEGLGDV+KGDNQNDSWIF LAV Sbjct: 61 KKKGFSLGSTVQSHTKGIWMWCVPHPKKPGHILVLLDTEGLGDVEKGDNQNDSWIFALAV 120 Query: 121 LLSSTLVYNSMGTINQQAMDQLYYVTELTHRIRSKSSPDENENE--DSADFVSFFPDFVW 178 LLSST VYNS+GTINQQAMDQLYYVTELTHRIRSKSSPDENENE DSADFVSFFPDFVW Sbjct: 121 LLSSTFVYNSIGTINQQAMDQLYYVTELTHRIRSKSSPDENENEVEDSADFVSFFPDFVW 180 Query: 179 TLRDFSLDLEADGQPLTPDEYLEYSLKLTQG 209 TLRDFSLDLEADGQPLTPDEYL YΞLKL +G Sbjct: 181 TLRDFSLDLEADGQPLTPDEYLTYSLKLKKG 211 Pedant information for DKFZphfbr2_78c24, frame 3 Report for DKFZphfbr2_78c24.3 [LENGTH] 563 [MW] 64127.72 [pi] 5.45 [HOMOL] PIR:A41268 guanine nucleotide-bmd ng protein 1 - human 0.0 [SUPFAM] guanine nucleotide-bindmg protein 1 0.0 [PROSITE] ATP_GTP_A 1 [PROSITE] RGD 1 [KW] TRANSMEMBRANE 1 [KW] LOW_COMPLEXITY 6.75 % [KW] COILED COIL 10.48 % SEQ MAPEIHMTGPMCLIENTNGELVANPEALKILSAITQPVVVVAIVGLYRTGKSYLMNKLAG SEG PRD cccccccccceeeeeccccchhhhhhhhhhhhhhhcceeeeeeeecccccchhhhhhhhh COILS MEM MMMMMMMMMMMMMMMMM SEQ KNKGFSLGSTVKSHTKGIWMWCVPHPKKPEHTLVLLDTEGLGDVKKGDNQNDSWIFTLAV SEG PRD cccccccccccccccceeeeeecccccccceeeeeeeccccccccccccccchhhhhhhh COILS MEM SEQ LLSSTLVYNSMGTINQQAMDQLYYVTELTHRIRSKSSPDENENEDSADFVSFFPDFVWTL SEG PRD hhhhheeeccccchhhhhhhhhhhhhhhhhhhhhcccccccccccccceeeeccceeeeh COILS MEM SEQ RDFSLDLEADGQPLTPDEYLEYSLKLTQGNRKLAQLEKLQDEELDPEFVQQVADFCSYIF SEG PRD hhhhhhhhccccccccchhhhhhhhhhccchhhhhhhhhhhhhcccchhhhhhhhhhhhc COILS MEM SEQ SNΞKTKTLSGGIKVNGPCLESLVLTYINAISRGDLPCMENAVLALAQIENSAAVQKAIAH SEG PRD cccceeeccccccccccchhhhhhhhhhhcccccccchhhhhhhhhhhhhhhhhhhhhhh COILS MEM SEQ YDQQMGQKVQLPAETLQELLDLHRVSEREATEVYMKNSFKDVDHLFQKKLAAQLDKKRDD SEG PRD hhhhhhhhhhcccchhhhhhhhhhhhhhhhhhhhhhccccchhhhhhhhhhhhhhhhhhh COILS MEM SEQ FCKQNQEASSDRCSALLQVIFSPLEEEVKAGIYSKPGGYCLFIQKLQDLEKKYYEEPRKG SEG PRD hhhhhhchhhhhhhhhhhhhhhhhhhhhhcccccccccceeehhhhhhhhhhhhhccccc COILS MEM SEQ IQAEEILQTYLKSKESVTDAILQTDQILTEKEKEIEVECVKAESAQASAKMVEEMQIKYQ SEG xxxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS MEM SEQ QMMEEKEKSYQEHVKQLTEKMERERAQLLEEQEKTLTSKLQEQARVLKERCQGESTQLQN SEG xxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccchhhhhhhh COILS cccccccccccccccccccccccccccccc cccccccccccccccccccccc MEM SEQ EIQKLQKTLKKKTKRYMSHKLKI SEG .. xxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhhhccc COILS CCCCCCC MEM Prosite for DKFZphfbr2_78c24.3 PS00016 272->275 RGD PDOC00016 PS00017 45->53 ATP GTP A PDOC00017 (No Pfam data available for DKFZphfbr2_78c24.3) DKFZphfbr2_78dl3 group: brain derived DKFZphfbr2_78dl3 encodes a novel 259 amino acid protein with similarity to C. elegans putative protein from cosmid K08B12. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of brain-specific genes . similarity to C. elegans K08B12.3 Sequenced by MediGenomix Locus: /map="338.4 cR from top of Chrl8 linkage group" Insert length: 2195 bp Poly A stretch at pos. 2175, polyadenylation signal at pos. 2156 1 CGTCCGTCGG GCAGCAGCGG GGCTGTCTAT CCCGGCTGAG GACCCGCGGC 51 CAGTGCGGGT GGCTGGCTTT GCCATTAGCG GGGGCCTTTC CTGAGGACGG 101 CGTACGGAGT GTGGGGAATG AAGGATGGCA GCATGCCGTG CATTAAAAGC 151 TGTTTTGGTA GATCTCAGTG GCACACTTCA CATTGAAGAT GCAGCTGTGC 201 CAGGCGCACA GGAAGCTCTT AAAAGGTTAC GTGGTGCTTC TGTAATCATT 251 AGGTTTGTGA CCAATACAAC CAAAGAGAGC AAGCAAGACC TGTTAGAAAG 301 GTTGAGAAAA TTGGAATTTG ATATCTCTGA AGATGAAATA TTCACATCTC 351 TGACTGCAGC CAGAAGTTTA CTAGAGCGGA AACAAGTCAG ACCCATGCTG 401 CTAGTTGATG ATCGGGCACT ACCTGATTTC AAAGGAATAC AAACAAGTGA 451 TCCTAATGCT GTGGTCATGG GATTGGCACC AGAACATTTT CATTATCAAA 501 TTCTGAATCA AGCATTCCGG TTACTCCTGG ATGGAGCACC TCTGATAGCA 551 ATCCACAAAG CCAGGTATTA CAAGAGGAAA GATGGCTTAG CCCTGGGGCC 601 TGGACCATTT GTGACTGCTT TAGAGTATGC CACAGATACC AAAGCCACAG 651 TCGTGGGGAA ACCAGAGAAG ACGTTCTTTT TGGAAGCATT GCGGGGCACT 701 GGCTGTGAAC CTGAGGAGGC TGTCATGATA GGAGATGATT GCAGGGATGA 751 TGTTGGTGGG GCTCAAGATG TCGGCATGCT GGGCATCTTA GTAAAGACTG 801 GGAAATATCG AGCATCAGAT GAAGAAAAAA TTAATCCACC TCCTTACTTA 851 ACTTGTGAGA GTTTCCCTCA TGCTGTGGAC CACATTCTGC AGCACCTATT 901 GTGAAGCAAT GTGTGCATCT GAAGCAACTT GAAATGCAGC TTCTTATTGT 951 CTGGAATGAA TCCCTTACCA ACTCAGTGCC AGCATCGGTA GACACCAGTC 1001 AGTGCTGATC GCTTTTTAAC CCTCTTTTGT TGTGCATTAA TTAGAAAGAA 1051 AGGTATTGAA TTGCGGCTAG CCAGTAAGCC TTGCTAATCT CTTTTATTTT 1101 GTAACTGAAG ATGAGACCCA AAGAAAGGGA AAGCTGAGAT TTTGTGCCAT 1151 TCCTTTTAAA ATATTCATCA GGTTAGGTGG GGCTGTGGGG GAAAAGCTAC 1201 TACAGGGAAG AGTGTTCTCT GCTGTCTCTT CACTGGAAAA CAGGGAGGGG 1251 GGATTTCAGA CTGTGAAGAA AGTTGAATGG TGGTTTTTAA ATTATAAAGT 1301 AATGTATTAA AAGGTGCATT AGGCTGTAGT TCTAATATTG AGTTCAACTG 1351 TGAAATCCAT CAGATGTGCC AAATGGAGAA GACAGAAAGC AACAAAGTGA 1401 ATTGTTCTTT AGCCCAAGTG GTACAGTGAA TTTGCTTTAA CAGATGTTGA 1451 AAACTAAATT TTCTACTGTA TTCCCAGCAC GGGTGACTTC TTTTTCTCTT 1501 CATTAGCCAG AGATGACTAA TTTAAATTTA GAACCAGATT TTAATTTAAA 1551 TTAATATTTC CATTAATAAC CTACTCATTG CAGATACCTA TTATACTGTG 1601 TAACAGTTGT TTTGGAAATT TTATGTAAAA TTAAAACTAT CAGTATTTTA 1651 CAGATGTTTT AATTAGACAT TGTTATTAAC AGGAACAGTG CAGAAACTAG 1701 AATCAAGCCT TATAATATCT TATAGACCAT GCATTTTTGA AGTTAGTGTC 1751 CACTAGGGTC CTATTAACTG TACATTTGCA AGATTTCATT ATTTTTGCCT 1801 CTGACACTAT GGGAAAAATT TTTTAGAAGC TATTGGGACA GATTCAAGCT 1851 TTTATGCACT TGGTTACTAC AGCTGTAAAA TGAAATCTCG TCTTGTAGCA 1901 TGGATTATTC TTCTCATGTT AAACCCACCA AAATAAAGGG GACTAAATAG 1951 GTAATGATTT TCCTAGTGCA TTTGCATACT GTGATAATCC TGGGCCTTGC 2001 AATAGTTCTA CAGGGCTCTT GGGCATTGAA TTATTAGGAT GTAATTGTAC 2051 ATCATTGTAG TGTTCACCTT ATTGAAGCTC ACTCTGATGT TAATGAGCTT 2101 CGGGTTTTGA TGCTTGTTTA GAGATCAGCA GTCTTGGATG GGAGGGAACA 2151 AAGCTAAATA AATGTTAGTT TGGTGAAAAA AAAAAAAAAA AAAAA BLAST Results Entry HS599355 from database EMBL: human STS WI-13484. Score = 1262, P = 3.6e-52, identities = 274/289 Medline entries No Medline entry Peptide information for frame 2 ORF from 125 bp to 901 bp; peptide length: 259 Category: similarity to unknown protein Classification: no clue 1 MAACRALKAV LVDLSGTLHI EDAAVPGAQE ALKRLRGASV IIRFVTNTTK 51 ESKQDLLERL RKLEFDISED EIFTSLTAAR SLLERKQVRP MLLVDDRALP 101 DFKGIQTSDP NA VMGLAPE HFHYQILNQA FRLLLDGAPL IAIHKARYYK 151 RKDGLALGPG PFVTALEYAT DTKATVVGKP EKTFFLEALR GTGCEPEEAV 201 MIGDDCRDDV GGAQDVGMLG ILVKTGKYRA SDEEKINPPP YLTCESFPHA 251 VDHILQHLL BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_78dl3, frame 2 TREMBL :CEUK08B12_1 gene: "K08B12.3"; Caenorhabditis elegans cosmid K08B12., N = 1, Score = 609, P = 2.2e-59 TREMBL:CEC13C4_5 gene: "C13C4.4"; Caenorhabditis elegans cosmid C13C4, N = 1, Score = 408, P = 4.4e-38 >TREMBL:CEUK08B12_1 gene: "K08B12.3"; Caenorhabditis elegans cosmid K08B12. Length = 257 HSPs: Score = 609 (91.4 bits), Expect = 2.2e-59, P = 2.2e-59 Identities = 132/251 (52%), Positives = 172/251 (68%) Query: 7 LKAVLVDLSGTLHIEDAAVPGAQEALKRLRGASVIIRFVTNTTKESKQDLLERLRKLEFD 66 + +VL+DLΞGT+HIE+ A+PGAQ AL+ LR + + +FVTNTTKESK+ L +RL F Sbjct: 4 ISSVLIDLSGTIHIEEFAIPGAQTALELLRQHAKV-KFVTNTTKESKRLLHQRLINCGFK 62 Query: 67 IΞEDEIFTSLTAARSLLERKQVRPMLLVDDRALPDFKGIQTSDPNAVVMGLAPEHFHYQI 126 + ++EIFTSLTAAR L+ + Q RP +VDDRA+ DF+GI T DPNAVV+GLAPE F+ Sbjct: 63 VEKEEIFTSLTAARDLIVKNQYRPFFIVDDRAMEDFEGISTDDPNAVVIGLAPEKFNDTT 122 Query: 127 LNQAFRLLLDG-APLIAIHKARYYKRKDGLALGPGPFVTALEYATDTKATVVGKPEKTFF 185 L AFRL+ + A LIAI+K RY++ GL LGPG +V LEY+ +AT+VGKP K FF Sbjct: 123 LTHAFRLIKEKKASLIAINKGRYHQTNAGLCLGPGTYVAGLEYSAGVEATIVGKPNKLFF 182 Query: 186 LEALRGTG--CEPEEAVMIGDDCRDDVGGAQDVGMLGILVKTGKYRASDEEKINPPPYLT 243 AL+ + AVMIGDD DD GA +GM ILVKTGK+R DE K+ Sbjct: 183 ESALQSLNENVDFSSAVMIGDDVNDDALGAIKIGMRAILVKTGKFRDGDELKVKN V 238 Query: 244 CESFPHAVDHILQH 257 SF AV+ I+++ Sbjct: 239 ANSFVDAVNMIIEN 252 Pedant information for DKFZphfbr2_78dl3, frame 2 Report for DKFZphfbr2_78dl3.2 [LENGTH] 259 [MW] 28536.04 [pi] 5.84 [HOMOL] TREMBL :CEUK08B12_1 gene: "K08B12.3"; Caenorhabditis elegans cosmid K08B12. 3e- 62 [FUNCAT] r general function prediction [M. jannaschn, MJ1437] 3e-05 [SUPFAM] nagD protein 4e-18 [KW] Alpha_Beta SEQ MAACRALKAVLVDLSGTLHIEDAAVPGAQEALKRLRGASVIIRFVTNTTKESKQDLLERL PRD ccccccceeeeeecccceeeecccccchhhhhhhhhhccceeeeeeccccchhhhhhhhh SEQ RKLEFDISEDEIFTSLTAARSLLERKQVRPMLLVDDRALPDFKGIQTSDPNAVVMGLAPE PRD hhhccccccceeeehhhhhhhhhhhhccceeeeeechhhhhhccccccccceeeeecccc SEQ HFHYQILNQAFRLLLDGAPLIAIHKARYYKRKDGLALGPGPFVTALEYATDTKATVVGKP PRD chhhhhhhhhhhhhhccceeeeeccccccccccccccccccchhhhhhhhccceeeeccc SEQ EKTFFLEALRGTGCEPEEAVMIGDDCRDDVGGAQDVGMLGILVKTGKYRASDEEKINPPP PRD cchhhhhhhhhhccccceeeeecccchhhhhhhhhccceeeeeeeccccccccccccccc SEQ YLTCESFPHAVDHILQHLL PRD cccccchhhhhhhhhhccc (No Prosite data available for DKFZphfbr2_78dl3.2) (No Pfam data available for DKFZphfbr2_78dl3.2) DKFZphfbr2_78k24 group: metabolism DKFZphfbr2_78k24 encodes a novel 372 ammo acid protein with similarity to Mus musculus ubiquitin specific protease UBP43. The novel protein contains a Prosite ubiquitin carboxyl-terminal hydrolases family 2 signature 2. Ubiquitin carboxyl-terminal hydrolases (EC 3.1.2.15) (UCH) (deubiquitinat g enzymes) are thiol proteases that recognize and hydrolyze the peptide bond at the C-terminal glycine of ubiquitin. These enzymes are involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins. The new protein can find application in modulation of protein stability/degradation in cells. Ubiquitin carboxyl-terminal hydrolases family 2 signature 2. strong similarity to mouse ubiquitin specific protease UBP43 Sequenced by MediGenomix Locus: unknown Insert length: 1874 bp Poly A stretch at pos. 1852, polyadenylation signal at pos. 1836 1 AGTCCCGACG TGGAACTCAG CAGCGGAGGC TGGACGCTTG CATGGCGCTT 51 GAGAGATTCC ATCGTGCCTG GCTCACATAA GCGCTTCCTG GAAGTGAAGT 101 CGTGCTGTCC TGAACGCGGG CCAGGCAGCT GCGGCCTGGG GGTTTTGGAG 151 TGATCACGAA TGAGCAAGGC GTTTGGGCTC CTGAGGCAAA TCTGTCAGTC 201 CATCCTGGCT GAGTCCTCGC AGTCCCCGGC AGATCTTGAA GAAAAGAAGG 251 AAGAAGACAG CAACATGAAG AGAGAGCAGC CCAGAGAGCG TCCCAGGGCC 301 TGGGACTACC CTCATGGCCT GGTTGGTTTA CACAACATTG GACAGACCTG 351 CTGCCTTAAC TCCTTGATTC AGGTGTTCGT AATGAATGTG GACTTCACCA 401 GGATATTGAA GAGGATCACG GTGCCCAGGG GAGCTGACGA GCAGAGGAGA 451 AGCGTCCCTT TCCAGATGCT TCTGCTGCTG GAGAAGATGC AGGACAGCCG 501 GCAGAAAGCA GTGCGGCCCC TGGAGCTGGC CTACTGCCTG CAGAAGTGCA 551 ACGTGCCCTT GTTTGTCCAA CATGATGCTG CCCAACTGTA CCTCAAACTC 601 TGGAACCTGA TTAAGGACCA GATCACTGAT GTGCACTTGG TGGAGAGACT 651 GCAGGCCCTG TATACGATCC GGGTGAAGGA CTCCTTGATT TGCGTTGACT 701 GTGCCATGGA GAGTAGCAGA AACAGCAGCA TGCTCACCCT CCCACTTTCT 751 CTTTTTGATG TGGACTCAAA GCCCCTGAAG ACACTGGAGG ACGCCCTGCA 801 CTGCTTCTTC CAGCCCAGGG AGTTATCAAG CAAAAGCAAG TGCTTCTGTG 851 AGAACTGTGG GAAGAAGACC CGTGGGAAAC AGGTCTTGAA GCTGACCCAT 901 TTGCCCCAGA CCCTGACAAT CCACCTCATG CGATTCTCCA TCAGGAATTC 951 ACAGACGAGA AAGATCTGCC ACTCCCTGTA CTTCCCCCAG AGCTTGGATT 1001 TCAGCCAGAT CCTTCCAATG AAGCGAGAGT CTTGTGATGC TGAGGAGCAG 1051 TCTGGAGGGC AGTATGAGCT TTTTGCTGTG ATTGCGCACG TGGGAATGGC 1101 AGACTCCGGT CATTACTGTG TCTACATCCG GAATGCTGTG GATGGAAAAT 1151 GGTTCTGCTT CAATGACTCC AATATTTGCT TGGTGTCCTG GGAAGACATC 1201 CAGTGTACCT ACGGAAATCC TAACTACCAC TGGCAGGAAA CTGCATATCT 1251 TCTGGTTTAC ATGAAGATGG AGTGCTAATG GAAATGCCCA AAACCTTCAG 1301 AGATTGACAC GCTGTCATTT TCCATTTCCG TTCCTGGATC TACGGAGTCT 1351 TCTAAGAGAT TTTGCAATGA GGAGAAGCAT TGTTTTCAAA CTATATAACT 1401 GAGCCTTATT TATAATTAGG GATATTATCA AAATATGTAA CCATGAGGCC 1451 CCTCAGGTCC TGATCAGTCA GAATGGATGC TTTCACCAGC AGACCCGGCC 1501 ATGTGGCTGC TCGGTCCTGG GTGCTCGCTG CTGTGCAAGA CATTAGCCCT 1551 TTAGTTATGA GCCTGTGGGA ACTTCAGGGG TTCCCAGTGG GGAGAGCAGT 1601 GGCAGTGGGA GGCATCTGGG GGCCAAAGGT CAGTGGCAGG GGGTATTTCA 1651 GTATTATACA ACTGCTGTGA CCAGACTTGT ATACTGGCTG AATATCAGTG 1701 CTGTTTGTAA TTTTTCACTT TGAGAACCAA CATTAATTCC ATATGAATCA 1751 AGTGTTTTGT AACTGCTATT CATTTATTCA GCAAATATTT ATTGATCATC 1801 TCTTCTCCAT AAGATAGTGT GATAAACACA GTCATGAATA AAGTTATTTT 1851 CCACAAAAAA AAAAAAAAAA AAAA BLAST Results Entry AC005500 from database EMBL: , complete sequence. Score = 859, P = 5.7e-143, identities = 175/179 8 exons matching Bp 317-1230 Medlme entries 99182491: A novel ubiquitm-specific protease, UBP43, cloned from leukemia fusion protein AMLl-ETO-expressing mice, functions in hematopoietic cell differentiation. Peptide information for frame 1 ORF from 160 bp to 1275 bp; peptide length: 372 Category: strong similarity to known protein Classification: Protein management Prosite motifs: UCH 2 2 (302-320) 1 MSKAFGLLRQ ICQSILAESS QSPADLEEKK EEDSNMKREQ PRERPRAWDY 51 PHGLVGLHNI GQTCCLNSLI QVFVMNVDFT RILKRITVPR GADEQRRSVP 101 FQMLLLLEKM QDSRQKAVRP LELAYCLQKC NVPLFVQHDA AQLYLKLWNL 151 IKDQITDVHL VERLQALYTI RVKDSLICVD CAMESSRNSS MLTLPLSLFD 201 VDSKPLKTLE DALHCFFQPR ELSSKSKCFC ENCGKKTRGK QVLKLTHLPQ 251 TLTIHLMRFS IRNSQTRKIC HSLYFPQSLD FSQILPMKRE SCDAEEQSGG 301 QYELFAVIAH VGMADSGHYC VYIRNAVDGK WFCFNDSNIC LVSWEDIQCT 351 YGNPNYHWQE TAYLLVYMKM EC BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_78k24, frame 1 TREMBLNEW:AF069502_1 product: "ubiquitin specific protease UBP43"; Mus musculus ubiquitin specific protease UBP43 mRNA, complete eds., N = 1, Score = 1367, P = le-139 SWISSPROT :UBPE_DROME UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 64E (EC 3.1.2.15) (UBIQUITIN THIOLESTERASE 64E) (UBIQUITIN-SPECIFIC PROCESSING PROTEASE 64E) (DEUBIQUITINATING ENZYME 64E) . , N = 2, Score = 248, P = 5.3e-33 >TREMBLNEW:AF069502_1 product: "ubiquitin specific protease UBP43"; Mus musculus ubiquitin specific protease UBP43 mRNA, complete eds. Length = 368 HSPs: Score = 1367 (205.1 bits), Expect = 1.0e-139, P = 1.0e-139 Identities = 262/369 (71%), Positives = 295/369 (79%) Query: 1 MSKAFGLLRQICQSILAESSQSPADLEEKKEEDSNMKREQPRERPRAWDYPHGLVGLHNI 60 M K FGLLR+ CQS++AE Q A LEE E KR R+ AWD PHGLVGLHNI Sbjct: 1 MGKGFGLLRKPCQSVVAEPQQYSA-LEE—ERTMKRKRVLSRDLCSAWDSPHGLVGLHNI 57 Query: 61 GQTCCLNSLIQVFVMNVDFTRILKRITVPRGADEQRRSVPFQMLLLLEKMQDSRQKAVRP 120 GQTCCLNSL+QVF+MN+DF ILKRITVPR A+E++RSVPFQ+LLLLEKMQDSRQKA+ P Sbjct: 58 GQTCCLNSLLQVFMMNMDFRMILKRITVPRSAEERKRSVPFQLLLLLEKMQDSRQKALLP 117 Query: 121 LELAYCLQKCNVPLFVQHDAAQLYLKLWNLIKDQITDVHLVERLQALYTIRVKDSLICVD 180 EL CLQK NVPLFVQHDAAQLYL +WNL KDQITD L ERLQ L+TI ++SLICV Sbjct: 118 TELVQCLQKYNVPLFVQHDAAQLYLTIWNLTKDQITDTDLTERLQGLFTIWTQESLICVG 177 Query: 181 CAMESSRNSSMLTLPLSLFDVDSKPLKTLEDALHCFFQPRELSSKSKCFCENCGKKTRGK 240 C ESSR S +LTL L LFD D+KPLKTLEDAL CF QP+EL+S C CE CG+KT K Sbjct: 178 CTAESSRRSKLLTLSLPLFDKDAKPLKTLEDALRCFVQPKELASSDMC-CETCGEKTPWK 236 Query: 241 QVLKLTHLPQTLTIHLMRFSIRNSQTRKICHSLYFPQSLDFSQILPMKRESCDAEEQSGG 300 QVLKLTHLPQTLTIHLMRFS RNS+T KICHS+ FPQSLDFSQ+LP + + D +EQS Sbjct: 237 QVLKLTHLPQTLTIHLMRFSARNSRTEKICHSVNFPQSLDFSQVLPTEEDLGDTKEQSEI 296 Query: 301 QYELFAVIAHVGMADSGHYCVYIRNAVDGKWFCFNDSNICLVSWEDIQCTYGNPNYHWQE 360 YELFAVIAHVGMAD GHYC YIRN VDGKWFCFNDS++C V+W+D+QCTYGN Y W+E Sbjct: 297 HYELFAVIAHVGMADFGHYCAYIRNPVDGKWFCFNDSHVCWVTWKDVQCTYGNHRYRWRE 356 Query: 361 TAYLLVYMK 369 TAYLLVY K Sbjct: 357 TAYLLVYTK 365 Pedant information for DKFZphfbr2_78k24, frame 1 Report for DKFZphfbr2_78k24.1 [LENGTH] 372 [MW] 43011.12 [pi] 8.05 [HOMOL] TREMBLNEW:AF069502_1 product: "ubiquitin specific protease UBP43 Mus musculus ubiquitin specific protease UBP43 mRNA, complete eds. le-151 [FUNCAT] 06.13 proteolysis [S. cerevisiae, YMR304w] 3e-19 [FUNCAT] 06.13.01 cytoplasmic degradation [S. cerevisiae, YJL197w] 3e-16 [FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylation, farnesylation and processing) [S. cerevisiae, YMR223w] le-15 [FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YNL186w] 6e-12 [FUNCAT] 03.10 sporulation and germination [S. cerevisiae, YDR069c] 9e-ll [FUNCAT] 10.03.99 other osmosensing activities [S. cerevisiae, YDR069c] 9e-ll [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YDR069c] 9e-ll [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YDR069c] 9e-ll [FUNCAT] 09.25 vacuolar and lysosomal biogenesis [S. cerevisiae, YDR069c] 9e-ll [BLOCKS] BL00582A Ribosomal protein L33 proteins [BLOCKS] BL00972E [BLOCKS] BL00972D [BLOCKS] BL00972A [EC] 2.4.2.29 Queuine tRNA-ribosyltransferase le-06 [PIRKW] pentosyltransferase le-06 [PIRKW] glycosyltransferase le-06 [PIRKW] tRNA modification le-06 [PIRKW] alternative splicing 7e-ll [PIRKW] hydrolase 7e-06 [SUPFAM] deubiquinatmg enzyme SSV7 2e-09 [PROSITE] UCH_2_21 [PFAM] Ubiquitin carboxyl-terminal hydrolases family 2 [PFAM] Ubiquitin carboxyl-terminal hydrolases family 2 [KW] Alpha_Beta SEQ MSKAFGLLRQICQSILAESSQSPADLEEKKEEDSNMKREQPRERPRAWDYPHGLVGLHNI PRD cccceeechhhhhhhhcccccccchhhhhhhhcccccccccccccccccccccccccccc SEQ GQTCCLNSLIQVFVMNVDFTRILKRITVPRGADEQRRSVPFQMLLLLEKMQDSRQKAVRP PRD cceeehhhhhhhhhcccchhhhhhhcccccccccchhhhhhhhhhhhhhhhhhhhccccc SEQ LELAYCLQKCNVPLFVQHDAAQLYLKLWNLIKDQITDVHLVERLQALYTIRVKDSLICVD PRD hhhhhccccccccchhhhhhhhhhhhhhhhhhhhhhcchhhhhhhhhhhhhhhhheeeee SEQ CAMESSRNSSMLTLPLSLFDVDSKPLKTLEDALHCFFQPRELSSKSKCFCENCGKKTRGK PRD ccccccccccccccccccccccccchhhhhhhhhhhhhhcccccccceeecccccccccc SEQ QVLKLTHLPQTLTIHLMRFSIRNSQTRKICHSLYFPQSLDFSQILPMKRESCDAEEQSGG PRD cceeeecccchhhhhhhhhhhccchhhhhccccccccccccccccccccccccccccccc SEQ QYELFAVIAHVGMADSGHYCVYIRNAVDGKWFCFNDSNICLVSWEDIQCTYGNPNYHWQE PRD eeeeeeeeeeeccccccceeeeeecccccceeeeccceeeeeecccccccccccccchhh SEQ TAYLLVYMKMEC PRD hhhhhhhhhccc Prosite for DKFZphfbr2_78k24.1 PS00973 302->320 UCH 2 2 PDOC00750 Pfam for DKFZphfbr2_78k24.1 HMM_NAME Ubiquitin carboxyl-terminal hydrolases family 2 HMM *GIqNlGNTCYMNSIIQCL* G+ N+G TC +NS+IQ+ Query 56 GLHNIGQTCCLNSLIQVF 73 HMM_NAME Ubiquitm carboxyl-terminal hydrolases family 2 HMM *YdLYgVICHYGntldyGHYWaYVKNenhHRWkWYYFDDEtV* Y+L++VI H G D+GHY +Y++N ++KW++F+D+++ Query 302 YELFAVIAHVG-MADSGHYCVYIRNAV--DGKWFCFNDSNI 339 DKFZphfbr2_78n23 group: brain derived DKFZphfbr2_78n23 encodes a novel 329 amino acid protein with similarity to A. thaliana F26P21.80 protein. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of brain-specific genes . similarity to A. thaliana F26P21.80 Sequenced by MediGenomix Locus: /map="89.1 cR from top of Chrl9 linkage group" Insert length: 1447 bp Poly A stretch at pos. 1374, polyadenylation signal at pos. 1353 1 TACAACTTCC GGCTGTAAAG ATGGCGGCTT CCTAGTGAGT CGGCGGCTGA 51 CTTAGAAGGA GGTTCAGGCT ACGGTGAGCC GAAGCCACAC AGGAGCCATG 101 GAAGTGGCAG AGCCCAGCAG CCCCACTGAA GAGGAGGAGG AGGAAGAGGA 151 GCACTCGGCA GAGCCTCGGC CCCGCACTCG CTCCAATCCT GAAGGGGCTG 201 AGGACCGGGC AGTAGGGGCA CAGGCCAGCG TGGGCAGCCG CAGCGAGGGT 251 GAGGGTGAGG CCGCCAGTGC TGATGATGGG AGCCTCAACA CTTCAGGAGC 301 CGGCCCTAAG TCCTGGCAGG TGCCCCCGCC AGCCCCTGAG GTCCAAATTC 351 GGACACCAAG GGTCAACTGT CCAGAGAAAG TGATTATCTG CCTGGACCTG 401 TCAGAGGAAA TGTCACTGCC AAAGCTGGAG TCGTTCAACG GCTCCAAAAC 451 CAACGCCCTC AATGTCTCTC AGAAGATGAT TGAGATGTTC GTGCGGACAA 501 AACACAAGAT CGACAAAAGC CACGAGTTTG CACTGGTGGT GGTGAACGAT 551 GACACGGCCT GGCTGTCTGG CCTGACCTCC GACCCCCGCG AGCTCTGTAG 601 CTGCCTCTAT GATCTGGAGA CGGCCTCCTG TTCCACCTTC AATCTGGAAG 651 GACTTTTCAG CCTCATCCAG CAGAAAACTG AGCTTCCGGT CACAGAGAAC 701 GTGCAGACGA TTCCCCCGCC ATATGTGGTC CGCACCATCC TTGTCTACAG 751 CCGTCCACCT TGCCAGCCCC AGTTCTCCTT GACGGAGCCC ATGAAGAAAA 801 TGTTCCAGTG CCCATATTTC TTCTTTGACG TTGTTTACAT CCACAATGGC 851 ACTGAGGAGA AGGAGGAGGA GATGAGTTGG AAGGATATGT TTGCCTTCAT 901 GGGCAGCCTG GATACCAAGG GTACCAGCTA CAAGTATGAG GTGGCACTGG 951 CTGGGCCAGC CCTGGAGTTG CACAACTGCA TGGCGAAACT GTTGGCCCAC 1001 CCCCTGCAGC GGCCTTGCCA GAGCCATGCT TCCTACAGCC TGCTGGAGGA 1051 GGAGGATGAA GCCATTGAGG TTGAGGCCAC TGTCTGAACC ATCCCTGTAC 1101 ATCTGCACCT TCTTGTGCAA GGAAGTCCTT GGCCTAAAGC CTTGGTTCTC 1151 AAACTGGGTT CCTTGGGACC TCCGGGGTGG GGGGGTTCCA GGAGGCACGT 1201 AGGGTACCTT GCAGGGTCCT AGGAGGGAAA CCCAGGATTC CAGGAGGGAT 1251 CCCAGGAACT GTGGGCACCC ATTTTCTGTG TCTCCCAGCC CATTTCCACT 1301 CCTAGTTTGT CATGGATAAT TTTTGTTCTT CCCTGTGTGA TTTTTGCCAT 1351 CAAAATAAAA ATTTGAGACT CGTTAAAAAA AAAAAAAAAA AAAAAAAAAA 1401 AAAAAAAAAA AAAAAAAAAA AAAAAAGAAA AAAAAAAAAA AAAAAAA BLAST Results Entry HS806352 from database EMBL: human STS EST192543. Score = 1285, P = 2.5e-51, identities 263/266 Medl e entries No Medline entry Peptide information for frame 2 ORF from 98 bp to 1084 bp; peptide length: 329 Category: similarity to unknown protein Classification: no clue 1 MEVAEPSSPT EEEEEEEEHS AEPRPRTRSN PEGAEDRAVG AQASVGSRSE 51 GEGEAASADD GSLNTSGAGP KSWQVPPPAP EVQIRTPRVN CPEKVIICLD 101 LSEEMSLPKL ESFNGSKTNA LNVSQKMIEM FVRTKHKIDK SHEFALVVVN 151 DDTAWLSGLT SDPRELCSCL YDLETASCST FNLEGLFSLI QQKTELPVTE 201 NVQTIPPPYV VRTILVYSRP PCQPQFSLTE PMKKMFQCPY FFFDVVYIHN 251 GTEEKEEEMS WKDMFAFMGS LDTKGTSYKY EVALAGPALE LHNCMAKLLA 301 HPLQRPCQSH ASYSLLEEED EAIEVEATV BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_78n23, frame 2 PIR:T05304 hypothetical protein F26P21.80 - Arabidopsis thaliana, N = 1, Score = 142, P = 1.5e-07 >PIR:T05304 hypothetical protein F26P21.80 - Arabidopsis thaliana Length = 264 HSPs: Score = 142 (21.3 bits), Expect = 1.5e-07, P = 1.5e-07 Identities = 56/216 (25%), Positives = 97/216 (44%) Query: 93 EKVIICLDL-SEEMSLPKLESFNGSKTNALNVSQKMIEMFVRTKHKIDKSHEFALVVVND 151 E ++IC+D+ +E M K NG + ++ I +F+ K 1+ H FA + Sbjct: 26 EDILICIDVDAEΞMVEMKTTGTNGRPLIRMECVKQAIILFIHNKLSINPDHRFAFATLAK 85 Query: 152 DTAWLSG-LTSDPRELCSCLYDLE-TASCSTFNLEGLFSLIQQKTELPVTENVQTIPPPY 209 AWL TSD + L L S S +L LF Q+ ++ +N Sbjct: 86 SAAWLKKEFTSDAESAVASLRGLSGNKΞSSRADLTLLFRAAAQEAKVSRAQN R 138 Query: 210 VVRTILVYSRPPCQPQFΞLTEPMKKMFQCPYFFFDVVYIHNGTEEKEEEMSWKDMF-AFM 268 + R IL+Y R +P P+ + F DV+Y+H ++ + +D++ + + Sbjct: 139 IFRVILIYCRSSMRPTHEW—PLNQKL FTLDVMYLH DKPSPDNCPQDVYDSLV 189 Query: 269 GSLD--TKGTSYKYEVALAGPALELHNCMAKLLAHPLQRPCQ 308 +++ ++ Y +E G A + M+ LL HP QR Q Sbjct: 190 DAVEHVSEYEGYIFESG-QGLARSVFKPMSMLLTHPQQRCAQ 230 Pedant information for DKFZphfbr2_78n23, frame 2 Report for DKFZphfbr2_78n23.2 [LENGTH] 329 [MW] 36560.10 [pi] 4.60 [HOMOL] PIR:T05304 hypothetical protein F26P21.80 - Arabidopsis thaliana 7e-07 [KW] Alpha_Beta [KW] LOW_COMPLEXITY 9.73 % SEQ MEVAEPSSPTEEEEEEEEHSAEPRPRTRSNPEGAEDRAVGAQASVGSRSEGEGEAASADD SEG . xxxxxxxxxxxxxxxxxxxxxx PRD cccccccccchhhhhhhhhhhccccccccccccchhhhhhhhhhhccccccccccccccc SEQ GSLNTSGAGPKSWQVPPPAPEVQIRTPRVNCPEKVIICLDLSEEMSLPKLESFNGSKTNA SEG PRD ccccccccccccccccccccceeeccccccccceeeeeccccccccccccccccccccee SEQ LNVSQKMIEMFVRTKHKIDKSHEFALVVVNDDTAWLSGLTSDPRELCΞCLYDLETASCST SEG PRD ehhhhhhhhhhhhhhhccccccceeeeeeccchhhhhcccccchhhhhhhhhcccccccc SEQ FNLEGLFSLIQQKTELPVTENVQTIPPPYVVRTILVYSRPPCQPQFSLTEPMKKMFQCPY SEG PRD hhhhhhhhhhhhhhhhhhhhhcccccccccceeeeeeecccccccccccchhhhhheeee SEQ FFFDVVYIHNGTEEKEEEMSWKDMFAFMGSLDTKGTSYKYEVALAGPALELHNCMAKLLA SEG PRD eeeeeeeeccccchhhhhhhhhhhhhhhhcccccccceeeeecccccchhhhhhhhhhhh SEQ HPLQRPCQSHASYSLLEEEDEAIEVEATV SEG xxxxxxxxxx ... PRD hcccccccccchhhhhhhhhhhhhhhccc (No Prosite data available for DKFZphfbr2_78n23.2) (No Pfam data available for DKFZphfbr2_78n23.2) DKFZphfbr2_7a24 group: brain derived DKFZphfbr2_7a24 encodes a novel 142 ammo acid protein with similarity to the C-terminal part of transforming growth factor-beta activated kinases. The novel protein shows only similarity to the C-termmus of such kinases; no kinase domain is present . No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of brain-specific genes . similarity to C-termmus of TGF-beta-activated kinase complete CDNA, complete eds, EST hits Sequenced by GBF Locus: unknown Insert length: 1697 bp No poly A stretch found, no polyadenylation signal found 1 GGGGAGAGAG GGGTTGTGAA GGGAAGCGGA AGGGAAGGGA AGGGAGGTCC 51 CGTGGGACGC TGGGGTCTGG GGTAGAGCAG GTAGCAGCGT GCTGCCCTGA 101 CAGCTGTCTC CGCTCCTCAG ATTGTCAGTG GCTGCTATGC AGCAGGTGCA 151 GCCTGGTCTC TCACTGAGTC TCTACTCCAC AAAGGCAACG ACTGGCCAAG 201 GCAGTGGCTG GCTCTGGGTT ACACAAGTGC AGACACTCAA CTAAGTGAGC 251 TGGAAGACCC AGGAGAAGGC GGAGGCTCAG GTGCCCACAT GATCAGCACA 301 GCCAGGGTAC CTGCTGACAA GCCTGTACGC ATCGCCTTTA GCCTCAATGA 351 CGCCTCAGAT GATACACCCC CTGAAGACTC CATTCCTTTG GTCTTTCCAG 401 AATTAGACCA GCAGCTACAG CCCCTGCCGC CTTGTCATGA CTCCGAGGAA 451 TCCATGGAGG TGTTCAGACA GCACTGCCAA ATAGCAGAAG AATACCTTGA 501 GGTCAAAAAG GAAATCACCC TGCTTGAGCA AAGGAAGAAG GAGCTCATTG 551 CCAAGTTAGA TCAGGCAGAA GAGGAGAAGG TGGATGCTGC TGAGCTGGTT 601 CGGGAATTCG AGGCTCTGAC GGAGGAGAAT CGGACGTTGA GGTTGGCCCA 651 GTCTCAATGT GTGGAACAAC TGGAGAAACT TCGAATACAG TATCAGAAGA 701 GGCAGGGCTC GTCCTAACTT TAAATTTTTC AGTGTGAGCA TACGAGGCTG 751 ATGACTGCCC TGTGCTGGCC AAAAGATTTT TATTTTAAAT GAATAGTGAG 801 TCAGATCTAT TGCTTCTCTG TATTACCCAC ATGACAACTG TCTATAATGA 851 GTTTACTGCT TGCCAGCTTC TAGCTTGAGA GAAGGGATAT TTTAAATGAG 901 ATCATTAACG TGAAACTATT ACTAGTATAT GTTTTTGGAG ATCAGAATTC 951 TTTTCCAAAG ATATATGTTT TTTTCTTTTT TAGGAAGATA TGATCATGCT 1001 GTACAACAGG GTAGAAAATG GTAAAAATAG ACTATTGACT GACCCAGCTA 1051 AGAATCGCGG GCTGAGCAGA GTTAAACCAT GGGACAAACC CATAACATGT 1101 TCACCATAGT TTCACGTATG TGTATTTTTA AATTTCATGC CTTTAATATT 1151 TCAAATATGC TCAAATTTAA ACTGTCAGAA ACTTCTCTGC ATGTATTTAT 1201 ATTTGCCAGA GTATAAACTT TTATACTCTG ATTTTTATCC TTCAATGATT 1251 GATTATACTA AGAATAAATG GTCACATATC CTAAAAGCTT CTTCATGAAA 1301 TTATTAGCAG AAACCATGTT TGAAACCAAA GCACATTTGC CAATGCTAAC 1351 TGGCTGTTGT AATAATAAAC AGATAAGGCT GCATTTGCTT CATGCCATGT 1401 GACCTCACAG TAAACATCTC TGCCTTTGCC TGTGTGTGTT CTGGGGGAGG 1451 GGGGACATGG AAAAATATTG TTTGGACATT ACTTGGGTGA GTGCCCATGA 1501 AGACATCAGT GAACTTGTAA CTATTGTTTT GTTTTGGATT TAAGGAGATG 1551 TTTTAGATCA GTAACAGCTA ATAGGAATAT GCGAGTAAAT TCAGAATTGA 1601 AACAATTTCT CCTTGTTCTA CCTATCACCA CATTTTCTCA AATTGAACTC 1651 TTTGTTATAT GTCCATTTCT ATTCATGTAA CTTCTTTTTC ATTAAAC BLAST Results No BLAST result Medline entries 98130593: Role of TAK1 and TAB1 in BMP signaling in early Xenopus development . Peptide information for frame 1 ORF from 289 bp to 714 bp; peptide length: 142 Category: similarity to known protein 1 MISTARVPAD KPVRIAFSLN DASDDTPPED SIPLVFPELD QQLQPLPPCH 51 DSEESMEVFR QHCQIAEEYL EVKKEITLLE QRKKELIAKL DQAEEEKVDA 101 AELVREFEAL TEENRTLRLA QSQCVEQLEK LRIQYQKRQG SS BLASTP hits Entry U92030_l from database TREMBL: product: "TAK1"; Xenopus laevis TGF-beta-activated kinase TAK1 mRNA, complete eds. Score = 343, P = 1.3e-30, identities = 69/143, positives = 104/143 Entry AB009356_1 from database TREMBL: product: "TGF-beta activated kinase la"; Homo sapiens mRNA for TGF-beta activated kinase la, complete eds. Score = 339, P = 2.6e-30, identities = 67/143, positives = 104/143 Entry MMPK_1 from database TREMBL: product: "TAK1 (TGF-beta-activated kinase)"; Mouse mRNA for TAK1 (TGF-beta-activated kinase), complete eds. Score = 339, P = 2.6e-30, identities = 67/143, positives = 104/143 Entry AB009357_1 from database TREMBL: product: "TGF-beta activated kinase lb"; Homo sapiens mRNA for TGF-beta activated kinase lb, complete eds. Score = 339, P = 3.2e-30, identities = 67/143, positives = 104/143 Entry AB009358_1 from database TREMBL: product: "TGF-beta activated kinase lc"; Homo sapiens mRNA for TGF-beta activated kinase lc, complete eds. Score = 144, P = 3.8e-09, identities = 30/67, positives = 47/67 Alert BLASTP hits for DKFZphfbr2_7a24, frame 1 PIR:JC5955 transforming growth factor-beta activated kinase (EC -.-.-.-) la - Human, N = 1, Score = 339, P = 3e-30 >PIR:JC5955 transforming growth factor-beta activated kinase (EC -.-.-.-) la - Human Length = 579 HSPs: Score = 339 (50.9 bits), Expect = 3.0e-30, P = 3.0e-30 Identities = 67/143 (46%), Positives = 104/143 (72%) Query: 1 MISTARVPADKPVRI-AFSLNDASDDTPPEDSIPLVFPELDQQLQPLPPCHDSEESMEVF 59 MI+T+ ++KP R ++ +D++D ++SIP+ + LD QLQPL PC +S+ESM VF Sbjct: 437 MITTSGPTSEKPTRSHPWTPDDSTDTNGSDNSIPMAYLTLDHQLQPLAPCPNSKESMAVF 496 Query: 60 RQHCQIAEEYLEVKKEITLLEQRKKELIAKLDQAEEEKVDAAELVREFEALTEENRTLRL 119 QHC++A+EY++V+ El LL QRK+EL+A+LDQ E+++ + + LV+E + L +EN++L Sbjct: 497 EQHCKMAQEYMKVQTEIALLLQRKQELVAELDQDEKDQQNTSRLVQEHKKLLDENKSLST 556 Query: 120 AQSQCVEQLEKLRIQYQKRQGSS 142 QC +QLE +R Q QKRQG+Ξ Sbjct: 557 YYQQCKKQLEVIRSQQQKRQGTS 579 Pedant information for DKFZphfbr2_7a24, frame 1 Report for DKFZphfbr2_7a24.1 [LENGTH] 142 [MW] 16377.53 [pi] 4.64 [HOMOL] TREMBL :U92030_1 product: "TAK1"; Xenopus laevis TGF-beta-activated kinase TAK1 mRNA, complete eds. 6e-26 [PROSITE] CK2_PHOSPHO_SITE 3 [PROSITE] PKC_PHOSPHO_SITE 2 [PROSITE] ASN_GLYCOSYLATION 1 [PFAM] TNFR/NGFR cysteme-rich region [KW] All_Alpha [KW] LOW_COMPLEXITY 7.04 % [KW] COILED COIL 33.10 % SEQ MISTARVPADKPVRIAFSLNDASDDTPPEDSIPLVFPELDQQLQPLPPCHDSEESMEVFR SEG xxxxxxxxxx PRD ccccccccccccccccccccccccccccccccccchhhhhhhhcccccccccchhhhhhh COILS SEQ QHCQIAEEYLEVKKEITLLEQRKKELIAKLDQAEEEKVDAAELVREFEALTEENRTLRLA SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccchhhhh COILS ...CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC SEQ QΞQCVEQLEKLRIQYQKRQGSS SEG PRD hhhhhhhhhhhhhhhhhhhccc COILS Prosite for DKFZphfbr2_7a24.1 PS00001 114->118 ASN GLYCOSYLATION PDOC00001 PS00005 4->7 PKC PHOSPHO SITE PDOC00005 PS00005 116->119 PKC PHOSPHO SITE PDOC00005 PS00006 18->22 CK2 PHOSPHO SITE PDOC00006 PS00006 26->30 CK2 PHOSPHO SITE PDOC00006 PS00006 77->81 CK2 PHOSPHO SITE PDOC00006 Pfam for DKFZphfbr2_7a24.1 HMM_NAME TNFR/NGFR cysteine-rich region HMM *CpeGtYtDWNHvpqClpCtrCePEMGQYMvqPCTwTQNTVC* C++++ + + +Q C++ E+ ++++++ T + ++ Query 49 CHDSEESMEVF-RQH—CQIAEE—YLEVKKEITLLEQRKK 84 DKFZphfbr2_7e22 group: brain derived DKFZphfbr2_7e22.2 encodes a novel 286 ammo acid protein similar to b561 cytochromes The new protein shows strong similarity to B561 cytochromes, but contains no heme binding site. In addition, a myc-type, helix-loop-helix dimerization domain domain is present. This helix-loop-helix domain mediates protein dimerization and has been found in proteins such as the myc family of cellular oncogenes, proteins involved in myogenesis and vertebrate proteins that bind specific DNA sequences in various lmmunoglobulin chains enhancers. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of bram-specific genes . strong similarity to cytochrome b561 complete cDNA, complete eds, EST hits Sequenced by GBF Locus : unknown Insert length: 4254 bp Poly A stretch at pos. 4234, polyadenylation signal at pos. 4217 1 GGGGACTACC CAGAGGGCTG CCGCCGCCTC TCCAAGTTCT TGTGGCCCCC 51 GCGGTGCGGA GTATGGGGCG CTGATGGCCA TGGAGGGCTA CCGGCGCTTC 101 CTGGCGCTGC TGGGGTCGGC ACTGCTCGTC GGCTTCCTGT CGGTGATCTT 151 CGCCCTCGTC TGGGTCCTCC ACTACCGAGA GGGGCTTGGC TGGGATGGGA 201 GCGCACTAGA GTTTAACTGG CACCCAGTGC TCATGGTCAC CGGCTTCGTC 251 TTCATCCAGG GCATCGCCAT CATCGTCTAC AGACTGCCGT GGACCTGGAA 301 ATGCAGCAAG CTCCTGATGA AATCCATCCA TGCAGGGTTA AATGCAGTTG 351 CTGCCATTCT TGCAATTATC TCTGTGGTGG CCGTGTTTGA GAACCACAAT 401 GTTAACAATA TAGCCAATAT GTACAGTCTG CACAGCTGGG TTGGACTGAT 451 AGCTGTCATA TGCTATTTGT TACAGCTTCT TTCAGGTTTT TCAGTCTTTC 501 TGCTTCCATG GGCTCCGCTT TCTCTCCGAG CATTTCTCAT GCCCATACAT 551 GTTTATTCTG GAATTGTCAT CTTTGGAACA GTGATTGCAA CAGCACTTAT 601 GGGATTGACA GAGAAACTGA TTTTTTCCCT GAGAGATCCT GCATACAGTA 651 CATTCCCGCC AGAAGGTGTT TTCGTAAATA CGCTTGGCCT TCTGATCCTG 701 GTGTTCGGGG CCCTCATTTT TTGGATAGTC ACCAGACCGC AATGGAAACG 751 TCCTAAGGAG CCAAATTCTA CCATTCTTCA TCCAAATGGA GGCACTGAAC 801 AGGGAGCAAG AGGTTCCATG CCAGCCTACT CTGGCAACAA CATGGACAAA 851 TCAGATTCAG AGTTAAACAA TGAAGTAGCA GCAAGGAAAA GAAACTTAGC 901 TCTGGATGAG GCTGGGCAGA GATCTACCAT GTAAAATGTT GTAGAGATAG 951 AGCCATATAA CGTCACGTTT CAAAACTAGC TCTACAGTTT TGCTTCTCCT 1001 ATTAGCCATA TGATAATTGG GCTATGTAGT ATCAATATTT ACTTTAATCA 1051 CAAAGGATGG TTTCTTGAAA TAATTTGTAT TGATTGAGGC CTATGAACTG 1101 ACCTGAATTG GAAAGGATGT GATTAATATA AATAATAGCA GATATAAATT 1151 GTGGTTATGT TACCTTTATC TTGTTGAGGA CCACAACATT AGCACGGTGC 1201 CTTGTGCAGA ATAGATACTC AATATGTGAA TATGTGTCTA CTAGTAGTTA 1251 ATTGGATAAA CTGGCAGCAT CCCTGGCCTG TTGTCATGCA GTCATTTCCT 1301 GTTAATTCTG GGAGACAATG ATTTCACAAC TAGAGGGAAG CAGTCCTAAA 1351 AGTTTAAAAT CCGATAAGGA ATATCTGGGA CAGGGTTTAG ATCATGACTC 1401 TACACAGATA CCATGATGAG AGTATATTAA AGAAATTTAG GAAAGCACCT 1451 GGTTCCTTTC TCCCCATGCC TGCCTTCTGC TCCCTCCCCA GCTGGTTTGG 1501 GCTCAAATTG TCCCTGGAGA CTAGGGTTTA TGTTAGGGTA TTGATAGATT 1551 AGAGCAGGTG GTTGAAGAGA TCTTCTCTGG TCAGACTTGG AAGAATTTCC 1601 AAAAGTGAAG TTAGCCCCAA GACTTCCCTA GGGTTGATGT ACTTTATGAT 1651 CCAGATGCTA AACTTCTTAG AATGAAAATA TGCTTCAACA CTTAAGTAGC 1701 ATACACTGCC CTACAAACCT CAGAGAGCAC TTTTCCCCAA GTTCTTGTTT 1751 TTATTTTTGA AAGTACTCAC ACAGCACTTA CTATGCTCCA AACACTCCTC 1801 TAAGCACTTT ACACATATTA GCTCATTCAG TCCCCAGACA GACGGGATGA 1851 AGTAGGTATT GTTACTGTTC CCATTTTACA GGTGAGAGAT TTGAAGCCTG 1901 GGGAGGCTAG TAACTCACCC CAAGGTCACA CGGCTCATAC ATGGTGGGAC 1951 TGAGACTCAG ATGCAGGCAG TCTGGCACCT CAGTCTGGAT TCTAACCATT 2001 TCACTAAGCT ATTTTTGTCT TGTACTACTT TGACCCACCC CTGAATAAAC 2051 CTCAATTGCT GGAGTGGGGT GTAGTTATTA AAGGGATGCT TTTTACCTTT 2101 TGCTGTCTGC TGTGGCAGAT TCCCCAGATA ACCAAGGAAA AGGGGCCACC 2151 CATACCTGGA AATAGGCCAT AGGGCCCCTA CTACTGCCAA CAAGCCATGG 2201 CCTACCTTGA CACTTGTTTG ATCTTAAAAT TGTGTCTTGG TAACAAAAGA 2251 TTTGGACAGG CATATCTGTA GCTTTCAAGT TAATTAATTG CAATATTTTT 2301 TTCTTCAGGA TTTTAGCTGC TGAACAACTT TCAGTTTGGA GCTAAAAGAG 2351 ACCTGTCTCA TGGTCTGCCC TTCCCTGGGG CAATAGCTAG GGTCTTTCCT 2401 GATTTTTATG GAATTTTAGG GGATATTTTG AGCTTTGGGT TCTCAGTAGT 2451 GAATTGAGAC TTGGAGGTGA CTTTTCATGT TTGGAGTATC ATCTCTGTCT 2501 GGGCTCTGGG CTGACAAATT AAAACCTAGA GTAGTGCTTA TGCTGAAATG 2551 ATACTTTTCA TTTTTTGGTT GATTTTTTTG CCTTCCCTTC AATTTTAAAC 2601 TGAAGCATTT TAATGTGGGT AGAAACTCTA CACCAAATAC ACTAAACATT 2651 TTGGTGCTTA GTGGATTTCT TTTTAGGTAA CTGGTACTTA CTTCCAAAGA 2701 CTGAATACAA GCCACACTCC ATCATATCCC TTAAACTTCA TGAAAAACCA 2751 TTCAAGATCC CCTTGCTGCA ACACTGTTCT CTTCTTCTCT ACTAAATTCT 2801 ATTTCCAAAA TTGGTAATAG AGCCAGAAGG ATCCCCAGTA CCCAGCCCTC 2851 TGCCTGGCAC AAAGTGGTAG CACAATTAAA TTCAGTATGG GTGGAGCATG 2901 GTACAGTCTT GGTGCCATAG AAGGAGTAGT TGCATAGTCA CACATCATTT 2951 GATAAGTTGG ATGTTCCATT ACATAGAGGA ACACAAAATT CCAGGGTTTT 3001 TGGAGGAAGG GATTAGATAG CGACTAAGCC GCCAGAATTG AGGTGGCCAT 3051 TCCTTTTTGT ATAGGCTAAG AAACAGGTTA TCAGTGAAAA GTTAATTATG 3101 GCTTTGGCAC TAGAATAGCA CTGTTGCAAA GTATTTAAGC ACCCCCCATC 3151 TCAGCCCTTT ATTTTATCTT TCATGTGGGC TAATGTGAGG ATAATCTTAC 3201 AGATATTATA GGAATTTCTT TTCTATCTTT ATGAAAACAA CGTATATAAA 3251 ATATATCTAG AAAACCTTTG TTTGAGACTC TTATTTAATG GGCTTTTGAT 3301 TCTAATGATA ATTGTACCTT TATCTTTCAA AAGCTGATAT TTCCTACCTA 3351 AGCATCTCCC GAGAAAAATA TCTCATTAAA AAGCCCATAA ATAATAGGGG 3401 AGAAGAAAGC CTTAGGTATC AATTCCAAAA CAGTGATTGA AATTTCCCAA 3451 AATAATTATG GCTTCTGTCA TCTCCAGAGA TAATCTGGCT TGGTTTACCC 3501 CATAATCTAA TTTCAGAAAA GAAAGCTTTA TTTTAACACT CATCTGAATC 3551 AACATTAAAG CCTTTTCTCT CAAAGCGTTT ATTGAGAAAC TCAAATGAAT 3601 ATACTTTTTG AATTACTGTC ATCAAAAGTG TACGGCTTCC TGTGCTGCTT 3651 GTGTCAAATG GAACCTGCCC TCTAAAGCAC TTTCTTTCCT TTACTTGCGT 3701 GGTTTCATGT AAGCTGTGCT GTTTAGAAAC AACATCTCAG ACTTTACAAA 3751 GAAATGACAA AGAAGGCAAT TGCACTTTTT AAGGGATATC GACAAGCAGT 3801 TTCTGTTTTC TAAAGGACAA AATACAGAGT GTGTGTCATT TTTAATTAGA 3851 TTCTTTCCCC TGCTGAGTTG GAAATTCCAG TGCAGCACTG ATTGACCACA 3901 GTTGCCAATC TAAAAGCACA AAGACAGAAG TAAAGCTTTA TGCTAATTTT 3951 ATTTCAATAT GATAGAAAAT TTATCTTGGT ATGTCCTTTT TTAGATAACT 4001 CCAGCAGGAA ACTGTAACTG CTATGTCTTT AGGAAAACGT AGAAGAAAGA 4051 ACATTATTAT TCTTTAATTC CTACAAGGTA CTTGAAAACC TTAAGTGAAA 4101 AAGATTTCTA TCTTTTTATC TTGGCGCATT TATGGAAAAA ATATTAACTG 4151 TCCTGAATAT TTTATAATTT TGTAGGAAAA ATATGCATCT ATTTTTTCTT 4201 GACTTCTTTT ATATAGTAAT AAAAGTTATT TTGGAAAAAA AAAAAAAAAA 4251 AAAA BLAST Results Entry HSG20626 from database EMBL: human STS A005Z27. Score = 860, P = 3.0e-32, identities = 176/181 Medline entries 89030633: The structure of cytochrome b561, a secretory vesicle-specific electron transport protein. Peptide information for frame 2 ORF from 74 bp to 931 bp; peptide length: 286 Category: strong similarity to known protein Classification: unset 1 MAMEGYRRFL ALLGSALLVG FLSVIFALVW VLHYREGLGW DGΞALEFNWH 51 PVLMVTGFVF IQGIAIIVYR LPWTWKCSKL LMKSIHAGLN AVAAILAIIS 101 VVAVFENHNV NNIANMYSLH SWVGLIAVIC YLLQLLSGFS VFLLPWAPLS 151 LRAFLMPIHV YSGIVIFGTV IATALMGLTE KLIFSLRDPA YSTFPPEGVF 201 VNTLGLLILV FGALIFWIVT RPQWKRPKEP NSTILHPNGG TEQGARGSMP 251 AYSGNNMDKS DSELNNEVAA RKRNLALDEA GQRSTM BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_7e22, frame 2 SWISSPROT:C561 SHEEP CYTOCHROME B561 (CYTOCHROME B-561)., N = 1, Score = 460, P = 1.3e-43 PIR:S01167 cytochrome b561 - bovine, N = 1, Score = 457, P = 2.7e-43 SWISSPROT:C561_PIG CYTOCHROME B561 (CYTOCHROME B-561)., N = 1, Score 452, P = 9.1e-43 PIR:S53321 cytochrome B561 - human, N = 1, Score = 451, P = 1.2e-42 >SWISSPROT:C561_SHEEP CYTOCHROME B561 (CYTOCHROME B-561) Length = 252 HSPs: Score = 460 (69.0 bits), Expect = 1.3e-43, P = 1.3e-43 Identities = 96/218 (44%), Positives = 131/218 (60%) Query 18 LVGFLSVIFALVWVLHYREGLGWDGSALEFNWHPVLMVTGFVFIQGIAIIVYRLPWTWKC 77 L+G V W+ YR G+ W+ SAL+FN HP+ MV G VF+QG A++VYR+ Sbjct 23 LLGLTVVAMTGAWLGMYRGGIAWE-SALQFNVHPLCMVIGLVFLQGDALLVYRV—FRNE 79 Query 78 SKLLMKSIHAGLNAVAAILAIISVVAVFENHNVNNIANMYSLHSWVGLIAVICYLLQLLS 137 +K K +H L+ A ++A++ +VAVFE+H A++YSLHSW G++ + Q L Sbjct 80 AKRTTKVLHGLLHVFAFVIALVGLVAVFEHHRKKGYADLYSLHSWCGILVFALFFAQWLV 139 Query 138 GFSVFLLPWAPLSLRAFLMPIHVYSGIVIFGTVIATALMGLTEKLIFSLRDPAYSTFPPE 197 GFΞ FL P A SLR+ P HV+ G IF +ATAL+GL E L+F L YSTF PE Sbjct 140 GFSFFLFPGASFSLRSRYRPQHVFFGAAIFLLSVATALLGLKEALLFEL-GTKYSTFEPE 198 Query 198 GVFVNTLGLLILVFGALIFWIVTRPQWKRPKEPNSTIL 235 GV N LGLL+ F ++ +I+TR WKRP + L Sbjct 199 GVLANVLGLLLAAFATVVLYILTRADWKRPLQAEEQAL 236 Pedant information for DKFZphfbr2_7e22, frame 2 Report for DKFZphfbr2_7e22.2 [LENGTH] 286 [MW] 31638.58 [pi] 9.12 [HOMOL] SWISSPROT :C561_SHEEP CYTOCHROME B561 (CYTOCHROME B-561) . 4e-40 [PIRKW] transmembrane protein 9e-40 [KW] SIGNAL_PEPTIDE 40 [KW] TRANSMEMBRANE 5 [KW] LOW COMPLEXITY 4.90 % SEQ MAMEGYRRFLALLGSALLVGFLSVIFALVWVLHYREGLGWDGSALEFNWHPVLMVTGFVF SEG PRD ccchhhhhhhhhhhhhhhhhhhhcchhhhhhhhhccccccccccccccccchhhhhhhhh MEM MMMMMMMMMMMM SEQ IQGIAIIVYRLPWTWKCSKLLMKSIHAGLNAVAAILAIISVVAVFENHNVNNIANMYSLH SEG xxxxxxxxxxxxxx PRD ccccceeeeeccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccccccccceeecc MEM MMMMMMMMMMMMMM MMMMMMMMMMMMMMMMMMMMMMMMM SEQ SWVGLIAVICYLLQLLSGFSVFLLPWAPLSLRAFLMPIHVYSGIVIFGTVIATALMGLTE SEG PRD cccchhhhhhhhhhhhhhheeeeccccccccccccccceeeeeeeeeeehhhhhhhhhhh MEM ....MMMMMMMMMMMMMMMMMMMMM MMMMMMMMMMMMMMMMMMMMM ... SEQ KLIFSLRDPAYSTFPPEGVFVNTLGLLILVFGALIFWIVTRPQWKRPKEPNSTILHPNGG SEG PRD hhhhhhhccccccccccchhhhhhhhhhhhhhhheeeeeecccccccccccccccccccc MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM SEQ TEQGARGSMPAYSGNNMDKSDSELNNEVAARKRNLALDEAGQRSTM SEG PRD cccccccccccccccccccccchhhhhhhhhhhhhhhhhhhhcccc MEM (No Prosite data available for DKFZphfbr2_7e22.2) (No Pfam data available for DKFZphfbr2 7e22.2) DKFZphfbr2_7j4 group: brain derived DKFZphfbr2_7 4 encodes a novel 233 amino acid protein without similarity to known proteins. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of brain-specific genes . unknown complete cDNA, complete eds, 1 EST h t Sequenced by GBF Locus : unknown Insert length: 1050 bp Poly A stretch at pos. 1027, polyadenylation signal at pos. 1007 1 GGGGACACAA AGGGGTGGTC ACCCTGCCCT CACCTTGACC TGTAAGTTGC 51 CTAGGACAGT GGCCTGGTCC CAGGGGCTGT TGTGGGGAGT TGAAGAACAC 101 CCTGGCCTCC TCCATCATGT CGGCCAAGAG GGCAGAATTG AAGAAAACAC 151 ATCTGTGCAA GAACTACAAG GCAGTTTGCC TGGAATTGAA GCCAGAGCCG 201 ACCAAAACAT TTGATTACAA AGCAGTTAAA CAAGAAGGGC GGTTTACCAA 251 AGCAGGAGTG ACACAGGACC TAAAGAATGA ACTCAGGGAA GTGAGAGAAG 301 AGCTCAAGGA GAAAATGGAG GAGATAAAAC AGATAAAGGA TCTAATGGAC 351 AAGGATTTTG ATAAACTTCA CGAATTTGTG GAAATTATGA AGGAAATGCA 401 GAAAGATATG GATGAGAAGA TGGACATTTT AATAAATACA CAGAAGAACT 451 ATAAGCTTCC CCTTAGAAGA GCACCAAAGG AGCAGCAGGA ACTCAGGCTG 501 ATGGGAAAGA CTCACAGAGA ACCACAGCTC AGGCCCAAGA AAATGGATGG 551 AGCCAGTGGA GTCAATGGAG CACCCTGTGC TCTTCACAAG AAGACGATGG 601 CACCACAAAA AACAAAACAG GGCTCACTGG ATCCCCTTCA TCACTGTGGG 651 ACCTGCTGCG AGAAATGTTT GTTGTGTGCT CTAAAGAACA ACTACAATCG 701 GGGGAACATT CCTTCAGAGG CCTCAGGCCT TTACAAAGGT GGAGAGGAGC 751 CAGTGACCAC CCAACCTTCT GTGGGCCACG CTGTGCCTGC CCCAAAGTCC 801 CAGACTGAGG GAAGGTGAAG CTTAACTGCC AGCTTGAAAT GAGAGTAAAG 851 AAGATACAGA GCAAACAGTG TTTCAGAAAC TGTCCTGCCC TGGGTGTGAT 901 TCTTTGGCTT CAATTTGAAG GAGGAGGAAT GATGGGATTT CATATTTTAT 951 TTCACACCAG TTCCTCCTTG TTTCATCTCT TTGCTAAGCT GGCTGCTTCT 1001 ACCATCTAAT AAATAATTGG CCAAGTTAAA AAAAAAAAAA AAAAAAAAAA BLAST Results No BLAST result Medl e entries No Medline entry Peptide information for frame 3 ORF from 117 bp to 815 bp; peptide length: 233 Category: putative protein 1 MSAKRAELKK THLCKNYKAV CLELKPEPTK TFDYKAVKQE GRFTKAGVTQ 51 DLKNELREVR EELKEKMEEI KQIKDLMDKD FDKLHEFVEI MKEMQKDMDE 101 KMDILINTQK NYKLPLRRAP KEQQELRLMG KTHREPQLRP KKMDGASGVN 151 GAPCALHKKT MAPQKTKQGS LDPLHHCGTC CEKCLLCALK NNYNRGNIPS 201 EASGLYKGGE EPVTTQPΞVG HAVPAPKSQT EGR BLASTP hits Entry JC2223 from database PIR: major surface glycoprotein 3 - Pneumocystis carinii (fragment) Score = 109, P = 3.5e-04, identities = 41/136, positives = 67/136 Alert BLASTP hits for DKFZphfbr2_7j4, frame 3 TREMBLNEW: PCP115C_1 product: "P115C"; Pneumocystis carinii mRNA for P115C, partial sequence., N = 1, Score = 109, P = 0.00024 >TREMBLNEW:PCP115C_1 product: "P115C"; Pneumocystis carmii mRNA for P115C, partial sequence. Length = 196 HSPs: Score = 109 (16.4 bits), Expect = 2.4e-04, P = 2.4e-04 Identities = 41/134 (30%), Positives = 67/134 (50%) Query: 14 CKN-YKAVCLELKPEPTKTFDYKAVKQEGRFTKA-GVTQDLKNELREVREELKEKMEEIK 71 CK K C ELK + K VK+ TK G ++LK+++++ E KE++E K Sbjct: 22 CKTELKKYCEELKEADGLKVNDK-VKEICDDTKRDGKCKELKDKVKKELETFKEELE—K 78 Query: 72 QIKDLMDKDFDKLHEFVEIMKEMQKDMDEKMDILINTQKNYKLPLRRAPKEQQELRLMGK 131 +KD+ D++ +K E +++E D D K + + + YKL +R E LR +GK Sbjct: 79 ALKDIKDENCEKYEEKCILLEETNHD-DVKKNCVKLREGCYKLKRKRVA-EDLLLRALGK 136 Query: 132 THREPQLRPKKMDGAS 147 + + K D S Sbjct: 137 DVKNGECEKKMKDVCS 152 Pedant information for DKFZphfbr2_7j4, frame 3 Report for DKFZphfbr2_7j4.3 [LENGTH] 233 [MW] 26533.95 [pi] 9.18 [PROSITE] MYRISTYL 3 [PROSITE] CK2 PHOSPHO SITE 3 [PROSITE] PKC PHOSPHO SITE 3 [KW] All Alpha [KW] LOW COMPLEXITY 14.59 % [KW] COILED COIL 13.73 % SEQ MSAKRAELKKTHLCKNYKAVCLELKPEPTKTFDYKAVKQEGRFTKAGVTQDLKNELREVR SEG xxxxxxxxx PRD ccchhhhhhhhhhccchhhhhhhcccccccccccceeecccccccccccchhhhhhhhhh COILS CCCCCCCCCCCC SEQ EELKEKMEEIKQIKDLMDKDFDKLHEFVEIMKEMQKDMDEKMDILINTQKNYKLPLRRAP SEG xxxxxxxx xxxxxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhcchhhhhhhhhhhhhhhhhhchhhhhhhhhcccccccccccc COILS CCCCCCCCCCCCCCCCCCCC SEQ KEQQELRLMGKTHREPQLRPKKMDGASGVNGAPCALHKKTMAPQKTKQGSLDPLHHCGTC SEG PRD hhhhhhhhhccccccccccccccccccccccccchhhhhhcccccccccccccccccccc COILS SEQ CEKCLLCALKNNYNRGNIPSEASGLYKGGEEPVTTQPSVGHAVPAPKSQTEGR SEG PRD chhhhhhhccccccccccccccccccccccccccccccccccccccccccccc COILS Prosite for DKFZphfbr2_7j4.3 PS00005 2->5 PKC_PHOSPHO_SITE PDOC00005 PS00005 108->111 PKC_PHOSPHO_SITE PDOC00005 PS00005 132->135 PKC_PHOSPHO_SITE PDOC00005 PS00006 132->136 CK2_PHOSPHO_SITE PDOC00006 PS00006 179->183 CK2_PHOΞPHO_SITE PDOC00006 PS00006 228->232 CK2_PHOSPHO_SITE PDOC00006 PS00008 151->157 MYRISTYL PDOC00008 PS00008 196->202 MYRISTYL PDOC00008 PΞ00008 204->210 MYRISTYL PDOC00008 (No Pfam data available for DKFZphfbr2_7j4.3) DKFZphfbr2_82c20 group: transmembrane protein DKFZphfbr2_82c20 encodes a novel 492 amino acid protein with very weak similarity to C. elegans cosmid D1007. The novel protein contains 7 transmembrane regions . No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of brain-specific genes and as a new marker for neuronal cells. similarity to C. elegans D1007.5 ; membrane regions : 7 Summary DKFZphfbr2_82c20 encodes a novel 492 ammo acid protein with similarity to a hypothetical C. elegans protein. similarity to C. elegans D1007.5 complete cDNA (Bp 1-100 GC ritch) , complete eds, potential start at Bp 128 matches Kozak consensus PyNNatgG, EST hits, localisation? primer B of STS doesn't match perfect! TRANSMEMBRANE 7 Sequenced by DKFZ Locus: /map="109.9 cR from top of Chrl linkage group"??? Insert length: 1804 bp Poly A stretch at pos. 1794, no polyadenylation signal found 1 CGGCGGGAGC GCGCGGCTGA TACCCGGGAC TGGGCTGCGG CGGTTAGTCC 51 TCTCCCGGCC GCCGTCGCCT CCGACATATT GCTCGCAGGA GCTGCGGCGG 101 CGAAGCGGAG AGCACCGGGG GGAGGAGATG GGAGGACGAA GAGGTCCCAA 151 CAGGACATCT TACTGTCGAA ATCCGCTCTG TGAGCCGGGA TCCTCGGGGG 201 GCTCTAGTGG AAGCCACACT TCCAGTGCAT CGGTGACCAG TGTTCGTTCC 251 CGCACCAGGA GCAGTTCTGG AACAGGCCTC TCCAGCCCTC CTCTGGCCAC 301 CCAAACTGTT GTGCCTCTAC AGCACTGCAA GATCCCCGAG CTGCCAGTCC 351 AGGCCAGCAT TCTGTTTGAG TTGCAGCTCT TCTTCTGCCA GCTCATAGCA 401 CTCTTCGTCC ACTACATCAA CATCTACAAG ACAGTGTGGT GGTATCCACC 451 TTCCCACCCA CCCTCCCACA CCTCCCTGAA CTTCCATCTG ATCGACTTCA 501 ACTTGCTGAT GGTGACCACC ATCGTTCTGG GCCGCCGCTT CATTGGGTCC 551 ATCGTGAAGG AGGCCTCTCA GAGGGGGAAG GTCTCCCTCT TTCGCTCCAT 601 CCTGCTGTTC CTCACTCGCT TCACCGTTCT CACGGCAACA GGCTGGAGTC 651 TGTGCCGATC CCTCATCCAC CTCTTCAGGA CCTACTCCTT CCTGAACCTC 701 CTGTTCCTCT GCTATCCGTT TGGGATGTAC ATTCCGTTCC TGCAGCTGAA 751 TTGCGACCTC CGCAAGACAA GCCTCTTCAA CCACATGGCC TCCATGGGGC 801 CCCGGGAGGC GGTCAGTGGC CTGGCAAAGA GCCGGGACTA CCTCCTGACA 851 CTGCGGGAGA CGTGGAAGCA GCACACAAGA CAGCTGTATG GCCCGGACGC 901 CATGCCCACC CATGCCTGCT GCCTGTCACC CAGCCTCATC CGCAGTGAGG 951 TGGAGTTCCT CAAGATGGAC TTCAACTGGC GCATGAAGGA AGTGCTCGTC 1001 AGCTCCATGC TGAGCGCCTA CTATGTGGCC TTTGTGCCTG TCTGGTTCGT 1051 GAAGAACACA CATTACTATG ACAAGCGCTG GTCCTGTGAA CTCTTCCTGC 1101 TGGTGTCCAT CAGCACCTCC GTGATCCTCA TGCAGCACCT GCTGCCTGCC 1151 AGCTACTGTG ACCTGCTGCA CAAGGCCGCC GCCCATCTGG GCTGTTGGCA 1201 GAAGGTGGAC CCAGCGCTGT GCTCCAACGT GCTGCAGCAC CCGTGGACTG 1251 AAGAATGCAT GTGGCCGCAG GGCGTGCTGG TGAAGCACAG CAAGAACGTC 1301 TACAAAGCCG TAGGCCACTA CAACGTGGCT ATCCCCTCTG ACGTCTCCCA 1351 CTTCCGCTTC CATTTCTTTT TCAGCAAACC TCTGCGGATC CTCAACATCC 1401 TCCTGCTGCT GGAGGGCGCT GTCATTGTCT ATCAGCTGTA CTCCCTAATG 1451 TCCTCTGAAA AGTGGCACCA GACCATCTCG CTGGCCCTCA TCCTCTTCAG 1501 CAACTACTAT GCCTTCTTCA AGCTGCTCCG GGACCGCTTG GTATTGGGCA 1551 AGGCCTACTC ATACTCTGCT AGCCCCCAGA GAGACCTGGA CCACCGTTTC 1601 TCCTGAGCCC TGGGGTCACC TCAGGGACAG CGTCCAGGCT TCAGCCAAGG 1651 GCTCCCTGGC AAGGGGCTGT TGGGTAGAAG TGGTGGTGGG GGGGACAAAA 1701 GACAAAAAAA TCCACCAGAG CTTTGTATTT TTGTTACGTA CTGTTTCTTT 1751 GATAATTGAT GTGATAAGGA AAAAAGTCCT ATTTTTATAC TCCCAAAAAA 1801 AAAA BLAST Results Entry HS285343 from database EMBL: human STS WI-17488. Score = 1225, P = 1.3e-50, identities = 263/281 Medline entries No Medline entry Peptide information for frame 2 1 MGGRRGPNRT SYCRNPLCEP GSSGGSSGSH TSSASVTSVR SRTRSSSGTG 51 LSSPPLATQT VVPLQHCKIP ELPVQAΞILF ELQLFFCQLI ALFVHYINIY 101 KTVWWYPPSH PPSHTSLNFH LIDFNLLMVT TIVLGRRFIG SIVKEASQRG 151 KVSLFRSILL FLTRFTVLTA TGWSLCRSLI HLFRTYΞFLN LLFLCYPFGM 201 YIPFLQLNCD LRKTSLFNHM ASMGPREAVS GLAKSRDYLL TLRETWKQHT 251 RQLYGPDAMP THACCLSPSL IRSEVEFLKM DFNWRMKEVL VSSMLSAYYV 301 AFVPVWFVKN THYYDKRWSC ELFLLVSIST SVILMQHLLP ASYCDLLHKA 351 AAHLGCWQKV DPALCSNVLQ HPWTEECMWP QGVLVKHSKN VYKAVGHYNV 401 AIPSDVSHFR FHFFFSKPLR ILNILLLLEG AVIVYQLYSL MSSEKWHQTI 451 SLALILFSNY YAFFKLLRDR LVLGKAYSYS ASPQRDLDHR FS ORF from 128 bp to 1603 bp; peptide length: 492 Category: similarity to unknown protein Prosite motifs: LEUCINE_ZIPPER (210-232) LEUCINE ZIPPER (210-232) BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_82c20, frame 2 TREMBL :CEAF3151_8 gene: "D1007.5"; Caenorhabditis elegans cosmid D1007., N = 2, Score = 247, P = 4.6e-29 >TREMBL:CEAF3151_8 gene: "D1007.5"; Caenorhabditis elegans cosmid D1007. Length = 512 HSPs: Score = 247 (37.1 bits), Expect = 4.6e-29, Sum P(2) = 4.6e-29 Identities = 58/204 (28%), Positives = 102/204 (50%) Query: 291 VSSMLSAYYVAFVPVWFVKNTHYYDKRWSCELFLLVSISTSVILMQHLLPAΞYCDLLHKA 350 +S ML +V F + ++ W C+L ++V ++ + + +L P +Y DLLH+A Sbjct: 299 LSIMLPCIFVPFKTSQGIPQKILINEVWECQLAIVVGLTAFSLYVAYLSPLNYLDLLHRA 358 Query: 351 AAHLGCWQKVD-PAL CSNVLQHPWTEECMWPQGVLVKHSKN-VYKAVGHYNV 400 A HLG W +++ P + + PW+E C++ G V+ Y+A ++ Sbjct: 359 AIHLGSWHQIEGPRIGHTGSMSSAPTPWΞEFCLYNDGETVQMPDGRCYRAKSSNSIRTVA 418 Query: 401 AIPSDVSHFRFHFFFSKPLRILNILLLLEGAVIVYQLYSLMSSEKWHQTISLALILFSNY 460 A P H F KP ++NI+ E +1 Q + L+ + W ++ L++F+NY Sbjct: 419 AHPESSRHNTFFKVLRKPNNLINIMCSFEFLLIFIQFWMLVLTNDWQHIVTFVLLMFANY 478 Query: 461 YAFFKLLRDRLVLGKAYΞYSASPQRDL 487 F KL +D+++L + Y S Q DL Sbjct: 479 LLFAKLFKDKIILSRIYEPS QEDL 502 Score = 178 (26.7 bits), Expect = 4.3e-21, Sum P(2) = 4.3e-21 Identities = 50/179 (27%), Positives = 90/179 (50%) Query: 262 HACCLSPSLIRSEVEFLKMDFNWRMKEVLVSSMLSAYYVAFVPVWFV—KNTHYYDKR-- 317 H C SP+ IR E++ L D R+K+ + + + +A+ +P FV K + ++ Sbjct: 262 HMCSDSPAQIREEIQVLIDDLVLRVKKSIFAGVSTAFLSIMLPCIFVPFKTSQGIPQKIL 321 Query: 318 WSCELFLLVSISTSVILMQHLLPASYCDLLHKAAAHLGCWQKVD-PAL CSNV 368 W C+L ++V ++ + + +L P +Y DLLH+AA HLG W +++ P + + Sbjct: 322 INEVWECQLAIVVGLTAFSLYVAYLSPLNYLDLLHRAAIHLGSWHQIEGPRIGHTGSMSS 381 Query: 369 LQHPWTEECMWPQGVLVKHSKN-VYKAVGHYNV-AIPSDVSHFRFHFFFSKPLRILNILL 426 PW+E C++ G V+ Y+A ++ + + R + FF K LR N L+ Sbjct: 382 APTPWSEFCLYNDGETVQMPDGRCYRAKSSNSIRTVAAHPESSRHNTFF-KVLRKPNNLI 440 Score = 146 (21.9 bits), Expect = 4.6e-29, Sum P(2) = 4.6e-29 Identities = 34/86 (39%), Positives = 50/86 (58%) Query: 52 SSPPLATQTVVPLQHCKIPELP-VQASILFELQLFFCQLIALFVHYINIYKTVWWYPPSH 110 +S P A+ + + H P++ Q + FE LF ++ALF+ Y+NIYKT+WW P S+ Sbjct: 19 ASIPRASGVTLSV-HPIWPDIQFTQGELFFECTLFLYSVLALFLQYLNIYKTLWWLPKSY 77 Query: 111 PPSHTSLNFHLIDFNLLMVTTIVLGRR 137 H SL FHLI+ L ++LG R Sbjct: 78 --WHYSLKFHLINPYFLSCVGLLLGWR 102 Score = 39 (5.9 bits), Expect = 6.8e-18, Sum P(2) = 6.8e-18 Identities = 12/41 (29%), Positives = 20/41 (48%) Query: 154 LFRSILLFLTRFTVLTATGWSLCRSLIHLFRTYΞFLNLLFL 194 L+ + LFL ++ + T W L +S H + +N FL Sbjct: 53 LYSVLALFL-QYLNIYKTLWWLPKSYWHYSLKFHLINPYFL 92 Pedant information for DKFZphfbr2_82c20, frame 2 Report for DKFZphfbr2_82c20.2 [LENGTH] 492 [MW] 56274.05 [pl] 9.51 [HOMOL] TREMB :CEAF3151 8 gene: "D1007.5"; Caenorhabditis elegans cosmid D1007. 4e-31 [PROSITE] LEUCINE_ZIPPER 1 [PROSITE] AMIDATION 2 [PROSITE] MYRISTYL 5 [PROSITE] CAMP_PHOSPHO_SITE 2 [PROSITE] CK2_PHOSPHO_SITE 3 [PROSITE] GLYCOSAMINOGLYCAN 1 [PROSITE] PKC_PHOSPHO_SITE 5 [PROSITE] ASN_GLYCOSYLATION 1 [KW] TRANSMEMBRANE 7 [KW] LOW COMPLEXITY 8.74 SEQ MGGRRGPNRTSYCRNPLCEPGSSGGSSGSHTSSASVTSVRSRTRSSSGTGLSSPPLATQT SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD ccccccccccccccccccccccccccccccccccccceeeccccccccccccccccccee MEM SEQ VVPLQHCKIPELPVQASILFELQLFFCQLIALFVHYINIYKTVWWYPPSHPPSHTSLNFH SEG PRD eeeccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhheeeeeccccccccceeeeee MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM MMMMMMMMM SEQ LIDFNLLMVTTIVLGRRFIGSIVKEASQRGKVΞLFRSILLFLTRFTVLTATGWSLCRSLI SEG PRD eeehhhhhhhhhhhhheeeehhhhhhhcccchhhhhhhhhhhhhhhhhhcccchhhhhhh MEM MMMMMMMMMMMMMMMMM MMMMMMMMMMMMMMMMMMMMMMMMMM SEQ HLFRTYSFLNLLFLCYPFGMYIPFLQLNCDLRKTSLFNHMASMGPREAVSGLAKSRDYLL SEG PRD hhhhhhhhheeeeeeecccccceeeeccccchhhhhhhhhhccchhhhhhhhhhhhhhhh MEM SEQ TLRETWKQHTRQLYGPDAMPTHACCLSPSLIRSEVEFLKMDFNWRMKEVLVSSMLSAYYV SEG PRD hhhhhhhhhhhhccccccccccccccccchhhhhhhhhhhhhhhhhhhhhhhcchhhhhh MEM MMMMMMMMMMMMMMMMM SEQ AFVPVWFVKNTHYYDKRWSCELFLLVSISTSVILMQHLLPASYCDLLHKAAAHLGCWQKV SEG PRD heeeeeeeeccccccchhhhhhhhhhhcchhhhhhhhhhccchhhhhhhhhhhhhhhccc MEM MMMMMMMMM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM SEQ DPALCSNVLQHPWTEECMWPQGVLVKHSKNVYKAVGHYNVAIPSDVSHFRFHFFFSKPLR SEG xx PRD ccccccccccccccceeecccceeeeeccceeeeccccccccccccccceeeeeecccch MEM MMMMMMMMMM SEQ ILNILLLLEGAVIVYQLYSLMSSEKWHQTISLALILFSNYYAFFKLLRDRLVLGKAYSYS SEG xxxxxxxx PRD hhhhhhhhhhheeeeehhhhhcccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccc MEM MMMMMMMMMMMMMMMMMMM MMMMMMMMMMMMMMMMMMMMM SEQ ASPQRDLDHRFS SEG PRD ccchhhhhhccc MEM Prosite for DKFZphfbr2_82c20.2 PS00001 8->12 ASN_GLYCOSYLATION PDOC00001 PS00002 47->51 GLYCOSAMINOGLYCAN PDOC00002 PS00004 212->216 CAMP_PHOSPHO_SITE PDOC00004 PS00004 316->320 CAMP_PHOSPHO_SITE PDOC00004 PS00005 38->41 PKC_PHOΞPHO_SITE PDOC00005 PS00005 147->150 PKC_PHOSPHO_SITE PDOC00005 PS00005 241->244 PKC_PHOSPHO_SITE PDOC00005 PS00005 245->248 PKC_PHOSPHO_SITE PDOC00005 PS00005 443->446 PKC_PHOSPHO_SITE PDOC00005 PS00006 241->245 CK2_PHOSPHO_SITE PDOC00006 PS00006 273->277 CK2_PHOSPHO_SITE PDOC00006 PS00006 342->346 CK2_PHOSPHO_SITE PDOC00006 PS00008 21->27 MYRISTYL PDOC00008 PS00008 24->30 MYRISTYL PDOC00008 PS00008 28->34 MYRISTYL PDOC00008 PS00008 48->54 MYRISTYL PDOC00008 PS00008 231->237 MYRISTYL PDOC00008 PS00009 2->6 AMIDATION PDOC00009 PS00009 134->138 AMIDATION PDOC00009 PS00029 168->190 LEUCINE ZIPPER PDOC00029 (No Pfam data available for DKFZphfbr2_82c20.2) DKFZphfbr2 82el7 group: transmembrane protein DKFZphfbr2_82el7 encodes a novel 311 amino acid protein with very weak similarity to C. elegans cosmid R01B10. The novel protein contains 6 transmembrane regions. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application n studying the expression profile of brain-specific genes and as a new marker for neuronal cells. similarity to C. elegans "R01B10.5" ; membrane regions: 6 Summary DKFZphfbr2_82el7 encodes a novel 311 amino acid protein with similarity to a hypothetical C. elegans protein. similarity to C. elegans "R01B10.5" cDNA, EST HS763158 extendes the sequence, complete eds, EST hits six potential transmembrane domains Sequenced by DKFZ Locus: /map="779_C_?; 818_A_1; 877_C_1; 734_C_12; 760_E_11; 171.7 cR from top of Chrl4 linkage group" Insert length: 1618 bp Poly A stretch at pos. 1608, polyadenylation signal at pos. 1588 1 CTGATCTAGT GCTTCTCGAA AAAAACCTTC AGGCGGCCCA TGGCTGTCGA 51 TATTCAACCA GCATGCCTTG GACTTTATTG TGGGAAGACC CTATTATTTA 101 AAAATGGCTC AACTGAAATA TATGGAGAAT GTGGGGTATG CCCAAGAGGA 151 CAGAGAACGA ATGCACAGAA ATATTGTCAG CCTTGCACAG AATCTCCTGA 201 ACTTTATGAT TGGCTCTATC TTGGATTTAT GGCAATGCTT CCTCTGGTTT 251 TACATTGGTT CTTCATTGAA TGGTACTCGG GGAAAAAGAG TTCCAGCGCA 301 CTTTTCCAAC ACATCACTGC ATTATTTGAA TGCAGCATGG CAGCTATTAT 351 CACCTTACTT GTGAGTGATC CAGTTGGTGT TCTTTATATT CGTTCATGTC 401 GAGTATTGAT GCTTTCTGAC TGGTACACGA TGCTTTACAA CCCAAGTCCA 451 GATTACGTTA CCACAGTACA CTGTACTCAT GAAGCCGTCT ACCCACTATA 501 TACCATTGTA TTTATCTATT ACGCATTCTG CTTGGTATTA ATGATGCTGC 551 TCCGACCTCT TCTGGTGAAG AAGATTGCAT GTGGGTTAGG GAAATCTGAT 601 CGATTTAAAA GTATTTATGC TGCACTTTAC TTCTTCCCAA TTTTAACCGT 651 GCTTCAGGCA GTTGGTGGAG GCCTTTTATA TTACGCCTTC CCATACATTA 701 TATTAGTGTT ATCTTTGGTT ACTCTGGCTG TGTACATGTC TGCTTCTGAA 751 ATAGAGAACT GCTATGATCT TCTGGTCAGA AAGAAAAGAC TTATTGTTCT 801 CTTCAGCCAC TGGTTACTTC ATGCCTATGG AATAATCTCC ATTTCCAGAG 851 TGGATAAACT TGAGCAAGAT TTGCCCCTTT TGGCTTTGGT ACCTACACCA 901 GCCCTTTTTT ACTTGTTCAC TGCAAAATTT ACCGAACCTT CAAGGATACT 951 CTCAGAAGGA GCCAATGGAC ACTGAGTGTA GACATGTGAA ATGCCAAAAA 1001 CCTGAGAAGT GCTCCTAATA AAAAAGTAAA TCAATCTTAA CAGTGTATGA 1051 GAACTATTCT ATCATATATG GGAACAAGAT TGTCAGTATA TCTTAATGTT 1101 TGGGTTTGTC TTTGTTTTGT TTATGGTTAG ACTTACAGAC TTGGAAAATG 1151 CAAAACTCTG TAATACTCTG TTACACAGGG TAATATTATC TGCTACACTG 1201 GAAGGCCGCT AGGAAGCCCT TGCTTCTCTC AACAGTTCAG CTGTTCTTTA 1251 GGGCAAAATC ATGTTTCTGT GTACCTAGCA ATGTGTTCCC ATTTTATTAA 1301 GAAAAGCTTT AACACGTGTA ATCTGCAGTC CTTAACAGTG GCGTAATTGT 1351 ACGTACCTGT TGTGTTTCAG TTTGTTTTTC ACCTATAATG AATTGTAAAA 1401 ACAAACATAC TTGTGGGGTC TGATAGCAAA CATAGAAATG ATGTATATTG 1451 TTTTTTGTTA TCTATTTATT TTCATCAATA CAGTATTTTG ATGTATTGCA 1501 AAAATAGATA ATAATTTATA TAACAGGTTT TCTGTTTATA GATTGGTTCA 1551 AGATTTGTTT GGATTATTGT TCCTGTAAAG AAAACAATAA TAAAAAGCTT 1601 ACCTACATAA AAAAAAAA BLAST Results Entry HS981146 from database EMBL: human STS WI-6253. Length = 208 Minus Strand HSPs: Score = 1040 (156.0 bits), Expect = 1.9e-40, 1.9e-40 Identities = 208/208 (100%), Positives = 208/208 (100%), Strand = Minus / Plus Entry HSG20716 from database EMBL: human STS A006D06. Length = 195 Minus Strand HSPs: Score = 975 (146.3 bits), Expect = 1.8e-37, P = 1.8e-37 Identities = 195/195 (100%), Positives = 195/195 (100%), Strand = Minus / Plus Medline entries No Medline entry Peptide information for frame 1 1 MAVDIQPACL GLYCGKTLLF KNGSTEIYGE CGVCPRGQRT NAQKYCQPCT 51 ESPELYDWLY LGFMAMLPLV LHWFFIEWYS GKKSSSALFQ HITALFECSM 101 AAIITLLVSD PVGVLYIRSC RVLMLSDWYT MLYNPSPDYV TTVHCTHEAV 151 YPLYTIVFIY YAFCLVLMML LRPLLVKKIA CGLGKSDRFK SIYAALYFFP 201 ILTVLQAVGG GLLYYAFPYI ILVLSLVTLA VYMSASEIEN CYDLLVRKKR 251 LIVLFSHWLL HAYGIISISR VDKLEQDLPL LALVPTPALF YLFTAKFTEP 301 SRILSEGANG H ORF from 40 bp to 972 bp; peptide length: 311 Category: similarity to unknown protein BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_82el7, frame 1 TREMBL:AF068718_5 gene: "R01B10.5"; Caenorhabditis elegans cosmid R01B10., N = 1, Score = 399, P = 1.4e-36 >TREMBL:AF068718_5 gene: "R01B10.5"; Caenorhabditis elegans cosmid R01B10. Length = 670 HSPs: Score = 399 (59.9 bits), Expect = 1.4e-36, P = 1.4e-36 Identities = 95/280 (33%), Positives = 152/280 (54%) Query: 2 AVDIQPACLGLYCGKTLLFKN GSTEIYGECGVCPRGQRTNAQKYCQPC 49 A IQP+CLG +CG+T+L N GST + CG C G R NA C+ C Sbjct: 292 ASTIQPSCLG-FCGRTVLVGNYSEDVEATTTAAGSTSL-SRCGPCSFGYRNNAMSICESC 349 Query: 50 TESPELYDWLYLGFMAMLPLVLHWFFIEWYSGKKSSSALFQ HITALFECSMAAIITL 106 + YDW+YL F+A+LPL+LH FI + K + ++ ++ + E +A +1 + Sbjct: 350 DTPLQPYDWMYLLFIALLPLLLHMQFIR-IARKYCRTRYYEVSEYLCVILENVIACVIAV 408 Query: 107 LVSDPVGVLYIRSCRVLMLSDWYTMLYNPSPDYVTTVHCTHEAVYPLYTIVFIYYAFCLV 166 L+ P ++ C + +WY YNP Y T+ CT+E V+PLY+I FI++ + Sbjct: 409 LIYPPRFTFFLNGCSKTDIKEWYPACYNPRIGYTKTMRCTYEVVFPLYSITFIHHLILIG 468 Query: 167 LMMLLRPLLVKKIACGLGKSDRFKSIYAALYFFPILTVLQAVGGGLLYYAFPYIILVLSL 226 +++LR L + L K+ K YAA+ PIL V+ AV G+++Y FPYI+L+ SL Sbjct: 469 SILVLRSTLYCVL LYKTYNGKPFYAAIVSVPILAVIHAVLSGVVFYTFPYILLIGSL 525 Query: 227 VTLAVYMSASEIENCYDLLVR KKRLIVLFSHWLLHAYGIISI 268 + +++ +++VR LI L L+ ++G+I+I Sbjct: 526 WAMCFHLALEGKRPLKEMIVRIATSPTHLIFLSITMLMLΞFGVIAI 571 Pedant information for DKFZphfbr2_82el7, frame 1 Report for DKFZphfbr2_82el7.1 [LENGTH] 311 [MW] 35239.14 [pi] 7.91 [HOMOL] TREMBL:AF068718_5 gene: "R01B10.5"; Caenorhabditis elegans cosmid R01B10. 9e-36 [PROSITE] AMIDATION 1 [PROSITE] MYRISTYL 3 [PROSITE] CAMP_PHOSPHO_SITE 1 [PROSITE] CK2_PHOSPHO_SITE 3 [PROSITE] PKC_PHOSPHO_SITE 4 [PROSITE] ASN_GLYCOSYLATION 1 [KW] TRANSMEMBRANE 6 [KW] LOW_COMPLEXITY 7.72 % SEQ MAVDIQPACLGLYCGKTLLFKNGSTEIYGECGVCPRGQRTNAQKYCQPCTESPELYDWLY SEG PRD cccccccccccccccceeeeccccceeecccccccccccccceeecccccccccchhhhh MEM MMMMMM SEQ LGFMAMLPLVLHWFFIEWYSGKKSSSALFQHITALFECSMAAIITLLVSDPVGVLYIRSC SEG PRD hhhhhhhhhhhhhhhhhhhccccchhhhhhhhhhhhhhhhhhhhhhhccccceeeeeece MEM MMMMMMMMMMMMMMMM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM... SEQ RVLMLSDWYTMLYNPSPDYVTTVHCTHEAVYPLYTIVFIYYAFCLVLMMLLRPLLVKKIA SEG xxxxxxxxxxxx .... PRD eeeeecceeeeecccccceeeeeeeceeeeeeeeceeeeehhhhhhhhhhhhhhhhhhee MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM... SEQ CGLGKSDRFKSIYAALYFFPILTVLQAVGGGLLYYAFPYIILVLSLVTLAVYMSASEIEN SEG PRD eecccccchhhhhhhhhhhccccccccccccceeeecceeeeehhhhhhhhhhhhhhhhh MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM SEQ CYDLLVRKKRLIVLFSHWLLHAYGIISISRVDKLEQDLPLLALVPTPALFYLFTAKFTEP SEG xxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhhhhhcccceeeechhhhhhceeeeeecccceeeeeeeccccc MEM MMMMMMMMMMMMMMMMMMMMM MMMMMMMMMMMMMMMMMMMMM SEQ SRILSEGANGH SEG PRD ceeeeeccccc MEM MM Prosite for DKFZphfbr2_82el7.1 PS00001 22->26 ASN_GLYCOSYLATION PDOC00001 PS00004 82->86 CAMP_PHOSPHO_SITE PDOC00004 PS00005 80->83 PKC_PHOSPHO_SITE PDOC00005 PS00005 119->122 PKC_PHOSPHO_SITE PDOC00005 PS00005 186->189 PKC_PHOSPHO_ΞITE PDOC00005 PS00005 294->297 PKC_PHOSPHO_SITE PDOC00005 PS00006 234->238 CK2_PHOSPHO_SITE PDOC00006 PS00006 236->240 CK2_PHOSPHO_SITE PDOC00006 PS00006 269->273 CK2_PHOSPHO_SITE PDOC00006 PS00008 11->17 MYRISTYL PDOC00008 PS00008 37->43 MYRISTYL PDOC00008 PS00008 182->188 MYRISTYL PDOC00008 PS00009 80->84 AMIDATION PDOC00009 (No Pfam data available for DKFZphfbr2_82el7.1) DKFZphfbr2_82e4 group: signal transduction DKFZphfbr2_82e4 encodes a novel 473 amino acid protein with strong similarity to the calmodulin-bmding proteins. The novel protein is similar to human and rat Ca2+/calmodulιn-dependent protein kinase (EC 2.7.1.123), rat calmodulin-bmding protein, calmodulin binding protein kinase of Fugu rupies and Rattus norvegicus calcium/calmodulm-dependent protein kinase I. Calmodulin is the archetype of the family of calcium-modulated proteins of which nearly 20 members have been found. Calmodulin is involved m regulation of growth and cell cycle as well as in signal transduction and the synthesis and release of neurotransmitters. The novel protein seems to be involved in calmodulm-mediated pathways m human neuronal cells. The new protein can find clinical application m modulating/blocking calmodulin-mediated pathways m human neuronal cells . strong similarity to calmodulin-bmding proteins complete cDNA, complete eds, EST hits splice variant in comparison to rat 156542 ESTs HSZZ54543/HS1141907 define splice variant see also DKFZphfbr2_82g20 unspliced form Sequenced by DKFZ Locus: /map="200.5 cR from top of Chr3 linkage group" Insert length: 2923 bp Poly A stretch at pos. 2913, polyadenylation signal at pos. 2890 1 ATGCTGGAGG TTCGCTAGCC GAAGCGGCTG CATCTGGCGC CGCGTCTGCC 51 CCGCGTGCTC GGAGCGGATT CTGCCCGCCG TCCCCGGAGC CCTCGGCGCC 101 CCGCTGAGCC CGCGATCACT TCCTCCCTGT GACCAACCGG CGCTGCAGGT 151 TAGAGCCTGG CAATGCCGTT TGGGTGTGTG ACTCTGGGTG ACAAGAAGAA 201 CTATAACCAG CCATCGGAGG TGACTGACAG ATATGATTTG GGACAGGTCA 251 TCAAGACTGA GGAGTTTTGT GAAATCTTCC GGGCCAAGGA CAAGACGACA 301 GGCAAGCTGC ACACCTGCAA GAAGTTCCAG AAGCGGGACG GCCGCAAGGT 351 GCGGAAAGCT GCCAAGAACG AGATAGGCAT CCTCAAGATG GTGAAGCATC 401 CCAACATCCT ACAGCTGGTG GATGTGTTTG TGACCCGCAA GGAGTACTTT 451 ATCTTCCTGG AGCTGGCCAC GGGGAGGGAG GTGTTTGACT GGATCCTGGA 501 CCAGGGCTAC TACTCGGAGC GAGACACAAG CAACGTGGTA CGGCAAGTCC 551 TGGAGGCCGT GGCCTATTTG CACTCACTCA AGATCGTGCA CAGGAATCTC 601 AAGCTGGAGA ACCTGGTTTA CTACAACCGG CTGAAGAACT CGAAGATTGT 651 CATCAGTGAC TTCCATCTGG CTAAGCTAGA AAATGGCCTC ATCAAGGAGC 701 CCTGTGGGAC CCCCGAGTAT CTGGGCAACC CACCTTTCTA TGAGGAGGTG 751 GAAGAAGATG ATTATGAGAA CCATGATAAG AATCTCTTCC GCAAGATCCT 801 GGCTGGTGAC TATGAGTTTG ACTCTCCATA TTGGGATGAT ATTTCGCAGG 851 CAGCCAAAGA CCTGGTCACA AGGCTGATGG AGGTGGAGCA AGACCAGCGG 901 ATCACTGCAG AAGAGGCCAT CTCCCATGAG TGGATTTCTG GCAATGCTGC 951 TTCTGATAAG AACATCAAGG ATGGTGTCTG TGCCCAGATT GAAAAGAACT 1001 TTGCCAGGGC CAAGTGGAAG AAGGCTGTCC GAGTGACCAC CCTCATGAAA 1051 CGGCTCCGGG CACCAGAGCA GTCCAGCACG GCTGCAGCCC AGTCGGCCTC 1101 AGCCACAGAC ACTGCCACCC CCGGGGCTGC AGGTGGGGCC ACAGCTGCAG 1151 CTGCGAGTGG AGCTACCTCA GCCCCTGAGG GTGATGCTGC TCGTGCTGCA 1201 AAGAGTGATA ATGTGGCCCC CGCAGACCGT AGTGCCACCC CAGCCACAGA 1251 TGGAAGTGCC ACCCCAGCCA CTGATGGCAG TGTCACCCCA GCCACCGATG 1301 GAAGCATCAC TCCAGCCACT GATGGGAGTG TCACCCCAGC CACTGACAGG 1351 AGCGCTACTC CAGCCACTGA TGGGAGAGCC ACACCAGCCA CAGAAGAGAG 1401 CACTGTGCCC ACCACCCAAA GCAGTGCCAT GCTGGCCACC AAGGCAGCTG 1451 CCACCCCTGA GCCGGCTATG GCCCAGCCGG ACAGCACAGC CCCAGAGGGC 1501 GCCACAGGCC AGGCTCCACC CTCTAGTAAA GGGGAAGAGG CTGCTGGTTA 1551 TGCCCAGGAG TCTCAAAGGG AGGAGGCCAG CTGAGTAGGC AGCCTGGTGA 1601 GGGGGGGCAG GGGATGGGCA GGAGGGTGGG AGAGTGGATG AGGGGCTTCT 1651 CACTGTACAT AGAGTCACTG GCATGATGCC CTCGCTCCCC CATGCCCCCA 1701 CATCCCAGTG GGGCATAACT AGGGGTCACG GGAGAGCAGT CTCGTCTCCT 1751 GTGTGTATGT GTGTGAGTGG TGGGCAGGCC AGTGGCAGGG CCGGCCCCAG 1801 CCCCTGCATG GATTCCTTGT GGCTTTTCTG TCTTTTGCTA GCTTCACCAG 1851 TTTCTGTTCC TTGTGGGATG CTGCTCTAGG GATACTCAGG GGGCTCCTGC 1901 TCTCCTTCCC CTTCCCTTCT TGCCTCACCA TTCCCCTAGG CAGGCCCTGC 1951 AGGTCCCACA CTCTCCCAGG CCCTAAACTT GGGCGGCCTT GCCCTGAGAG 2001 CTGGTCCTCC AGCGAGGCCC TGTCAGCGGT CTTAGGCTCC TGCACATGAA 2051 GGTGTGTGCC TGTGGTGTGT GGGCTGCTCT AGGAGCAGAT ACAGGCTGGT 2101 ATAGAGGATG CAGAAAGGTA GGGCAGTATG TTTAAGTCCA GACTTGGCAC 2151 ATGGCTAGGG ATACTGCTCA CTAGCTGTGG AGGTCCTCAG GAGTGGAGAG 2201 AATGAGTAGG AGGGCAGAAG CTTCCATTTT TGTCCTTCCT AAGACCCTGT 2251 TATTTGTGTT ATTTCCTGCC TTTCCGAGTC CTGCAGTGGG CTGCCCTGTA 2301 CCCTGAACCT CATGAGCCTC TAAGGGAAAG GAGGAACAAT TAGGACGTGG 2351 CAATGAGACC TGGCAGGGCA GAGTACAAGC CCAGCACCCA GTGTCCCAGC 2401 CTTACTGGGT CCTTACCCTG GGCCAAACAG GGAGGGCTGA TACCTCCTTG 2451 CTCTTCCTAG ATGCCCACCT CCTACAATCT CAGCCCACAA GTCCTCTCCA 2501 CCCTAGGGGG CTTGCTGCAT GGCAATAACT CATAATCTGA TTTGGAGGTT 2551 TGCCCTTTAC AGGGGCAGAT TTTCTGCTCA GTTCAACAAT GAAATGAAGA 2601 GGAACTCCCT CTTTCTACAG CTCACTTCTA TCAGAGGCCC AGGTGCCTCA 2651 GAGCCACATT GAGTTGCTTT TTCTGGGATG AGGAAGTAGG GTTAAACTCC 2701 CCAGTTTCCT GAGGGAGGCT CCTGACAGGT GCCCTTTGTC AGACCCTACC 2751 ACAGCCTGGA TAGGCAGCCA CATTGGTCCT CGCCCTTGCT CGGCACTCCG 2801 TGGTGGTCCT GCCCTTCTCC CTGCATGCCT GTGGGTCTGC TCTGGTGTGT 2851 GAAGGTCGGT GGGTTAACTG TGTGCCTACT GAACCTGGCA AATAAACATC 2901 ACCCTGCAAA GCCAAAAAAA AAA BLAST Results Entry HS452352 from database EMBL: human STS WI-15318. Length = 350 Minus Strand HSPs: Score = 1547 (232.1 bits), Expect = 5.2e-63, P = 5.2e-63 Identities = 331/348 (95%), Positives = 331/348 (95%), Strand = Minus / PI Medline entries 94110847: J Neurosci 1994 Jan; 14 ( 1) : 1-13 1G5 : a calmodulin-bmdmg, vesicle-associated, protein kinase-like protein enriched in forebrain neuπtes. Godbout M, Erlander MG, Hasel KW, Danielson PE, Wong KK, Battenberg EL, Foye PE, Bloom FE, Sutcliffe JG Peptide information for frame 1 1 MPFGCVTLGD KKNYNQPSEV TDRYDLGQVI KTEEFCEIFR AKDKTTGKLH 51 TCKKFQKRDG RKVRKAAKNE IGILKMVKHP NILQLVDVFV TRKEYFIFLE 101 LATGREVFDW ILDQGYYSER DTSNVVRQVL EAVAYLHSLK IVHRNLKLEN 151 LVYYNRLKNS KIVISDFHLA KLENGLIKEP CGTPEYLGNP PFYEEVEEDD 201 YENHDKNLFR KILAGDYEFD SPYWDDISQA AKDLVTRLME VEQDQRITAE 251 EAISHEWISG NAASDKNIKD GVCAQIEKNF ARAKWKKAVR VTTLMKRLRA 301 PEQSSTAAAQ SASATDTATP GAAGGATAAA ASGATSAPEG DAARAAKSDN 351 VAPADRSATP ATDGSATPAT DGSVTPATDG SITPATDGSV TPATDRSATP 401 ATDGRATPAT EESTVPTTQS SAMLATKAAA TPEPAMAQPD STAPEGATGQ 451 APPSSKGEEA AGYAQESQRE EAS ORF from 163 bp to 1581 bp; peptide length: 473 Category: strong similarity to known protein BLASTP hits Entry S50193 from database PIR: Ca2+/calmodulιn-dependent protein kinase (EC 2.7.1.123) I - rat Length = 374 Score = 371 (130.6 bits), Expect = 2.2e-66, Sum P(2) = 2.2e-66 Identities = 74/176 (42%), Positives = 115/176 (65%) Entry S57347 from database PIR: Ca2+/calmodulm-dependent protein kinase (EC 2.7.1.123) I - human Length = 370 Score = 369 (129.9 bits), Expect = 4.6e-66, Sum P(2) = 4.6e-66 Identities = 74/176 (42%), Positives = 114/176 (64%) Alert BLASTP hits for DKFZphfbr2_82e4, frame 1 PIR: 156542 calmodulin-bmdmg protein - rat, N = 2, Score = 1246, P 4e-228 TREMBLNEW: FRU010348_3 product: "calmodulin binding protein kinase"; Fugu rubripes UBEl-like gene, PRGFR2 gene and gene encoding calmodulin binding protein kinase, clone 168J21, N = 2, Score = 846, P = 2.6e-139 TREMBL :RNPRKI_1 product: "protein kinase I"; Rattus norvegicus calcium/calmodulm-dependent protein kinase I mRNA, complete eds., N = 2, Score = 364, P = 5. le-63 >PIR: 156542 calmodulm-binding protein Length = 504 HSPs: Score = 1246 (186.9 bits), Expect = 4.0e-228, Sum P(2) 4.0e-228 Identities = 255/289 (88%), Positives = 259/289 (89%) Query: 188 GNPPFYEEVEEDDYENHDKNLFRKILAGDYEFDSPYWDDISQAAKDLVTRLMEVEQDQRI 247 GNPPFYEEVEEDDYENHDKNLFRKILAGDYEFDSPYWDDISQAAKDLVTRLMEVEQDQRI Sbjct: 216 GNPPFYEEVEEDDYENHDKNLFRKILAGDYEFDSPYWDDISQAAKDLVTRLMEVEQDQRI 275 Query: 248 TAEEAISHEWISGNAASDKNIKDGVCAQIEKNFARAKWKKAVRVTTLMKRLRAPEQSSTA 307 TAEEAISHEWISGNAASDKNIKDGVCAQIEKNFARAKWKKAVRVTTLMKRLRAPEQS TA Sbjct: 276 TAEEAISHEWISGNAASDKNIKDGVCAQIEKNFARAKWKKAVRVTTLMKRLRAPEQSGTA 335 Query: 308 AAQSASATDTATPGAAGGATAAAASGATSAPE GDAARAAKSDNVAPADRSAT 359 A +D ATPGAAGGA AAAA GA A GDA AAKSD++A ADRSAT Sbjct: 336 AT SDAATPGAAGGAVAAAAGGAAPASGASATVGTGGDAGCAAKSDDMASADRSAT 390 Query: 360 PATDGSATPATDGSVTPATDGSITPATDGSVTPATDRSATPATDGRATPATEESTVPTTQ 419 PATDGSATPATDGSVTPATDGSITPATDGSVTPATDRSATPATDGRATPATEESTVP Q Sbjct: 391 PATDGSATPATDGSVTPATDGSITPATDGSVTPATDRSATPATDGRATPATEESTVPAAQ 450 Query: 420 SSAMLATKAAATPEPAMAQPDSTAPEGATGQAPPSSKGEEAAGYAQESQREEAS 473 SSA A KAAATPEPA+AQPDSTA EGATGQAPPSSKGEEA G AQESQR E S Sbjct: 451 SSAAPAAKAAATPEPAVAQPDSTALEGATGQAPPSSKGEEATGCAQESQRVETS 504 Score = 978 (146.7 bits), Expect = 4.0e-228, Sum P(2) = 4.0e-228 Identities = 186/187 (99%), Positives = 187/187 (100%) Query: 1 MPFGCVTLGDKKNYNQPSEVTDRYDLGQVIKTEEFCEIFRAKDKTTGKLHTCKKFQKRDG 60 MPFGCVTLGDKKNYNQPSEVTDRYDLGQV+KTEEFCEIFRAKDKTTGKLHTCKKFQKRDG Sbjct: 1 MPFGCVTLGDKKNYNQPSEVTDRYDLGQVVKTEEFCEIFRAKDKTTGKLHTCKKFQKRDG 60 Query: 61 RKVRKAAKNEIGILKMVKHPNILQLVDVFVTRKEYFIFLELATGREVFDWILDQGYYSER 120 RKVRKAAKNEIGILKMVKHPNILQLVDVFVTRKEYFIFLELATGREVFDWILDQGYYSER Sbjct: 61 RKVRKAAKNEIGILKMVKHPNILQLVDVFVTRKEYFIFLELATGREVFDWILDQGYYSER 120 Query: 121 DTSNVVRQVLEAVAYLHSLKIVHRNLKLENLVYYNRLKNSKIVISDFHLAKLENGLIKEP 180 DTSNVVRQVLEAVAYLHSLKIVHRNLKLENLVYYNRLKNSKIVISDFHLAKLENGLIKEP Sbjct: 121 DTSNVVRQVLEAVAYLHSLKIVHRNLKLENLVYYNRLKNSKIVISDFHLAKLENGLIKEP 180 Query: 181 CGTPEYL 187 CGTPEYL Sbjct: 181 CGTPEYL 187 Pedant information for DKFZphfbr2_82e4, frame 1 Report for DKFZphfbr2_82e4.1 [LENGTH] 473 [MW] 5120 8.89 [pi] 5.30 [HOMOL] PIR: 156542 calmodulin-bindmg protein - rat 0.0 [FUNCAT] 30.0 3 organization of cytoplasm [S. cerevisiae, YFR014c] 4e-30 [FUNCAT] 10.9 9 other signal-transduction activities [S. cerevisiae, YFR014c] 4e-30 [FUNCAT] 03.0 1 cell growth [S. cerevisiae, YFR014c] 4e-30 [FUNCAT] 30.1 0 nuclear organization [S. cerevisiae, YKLlOlw] 2e-26 [FUNCAT] 0 3 .2 2 cell cycle control and mitosis [S. cerevisiae, YKLlOlw] 2e-26 [FUNCAT] 11.0 dna repair (direct repair, base excision repair and nucleotide excision repair) [S. cerevisiae, YDLlOlc] 8e-26 [FUNCAT] 98 class fication not yet clear-cut [S. cerevisiae, YCL024w] 5e-24 [FUNCAT] 03.2 5 cytokinesis [S. cerevisiae, YDR507c] 7e-23 [FUNCAT] 03.0 4 budding, cell polarity and filament formation [S. cerevisiae, YDR507c] 7e-23 [FUNCAT] 03.22.01 cell cycle check point proteins [S. cerevisiae, YPL153c] le-21 [FUNCAT] 03.19 recombination and dna repair [S. cerevisiae, YPL153c] le-21 [FUNCAT] 11.01 stress response [S. cerevisiae, YDR477w] 3e-19 [FUNCAT] 01.05.04 regulation of carbohydrate utilization [S. cerevisiae, YDR477w] 3e-19 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YPL141c] le-16 [FUNCAT] 03. 16 dna synthesis and replication [S. cerevisiae, YMROOlc] 3e-16 [FUNCAT] 03. 13 meiosis [S. cerevisiae, YOR351c] le-15 [FUNCAT] 30.02 organization of plasma membrane [S. cerevisiae, YDR122w] 3e-14 [ FUNCA ] 10.03.11 key kinases [S. cerevisiae, YCR073c] 6e-ll [FUNCAT] 09.01 biogenesis of cell wall [S. cerevisiae, YNR031c] 8e-ll [FUNCAT] 10.02.11 key kinases [S. cerevisiae, YJL095w] 2e-09 [FUNCAT] 03.07 pheromone response, matmg-type determination, sex-specific proteins [S. cerevisiae, YLR362w] le-08 [FUNCAT] 10.05.11 key kinases [S. cerevisiae, YLR362w] le-08 [FUNCAT] 10.04.11 key kinases [S. cerevisiae, YLR362w] le-08 [FUNCAT] 02.19 metabolism of energy reserves (glycogen, trehalose) [S. cerevisiae, YPL031c] 7e-08 [FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YPL031c] 7e-08 [FUNCAT] 01.04.04 regulation of phosphate utilization [S. cerevisiae, YPL031c] 7e-08 [FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylation, farnesylation and processing) [S. cerevisiae, YFL033c] le-07 [FUNCAT] 04.99 other transcription activities [S. cerevisiae, YFL033c] le-07 [FUNCAT] 10.05.09 regulation of g-protem activity [S. cerevisiae, YBL016w] 5e-07 [FUNCAT] 05.07 translational control [S. cerevisiae, YDR283c] 8e-07 [FUNCAT] 01.06.10 regulation of lipid, fatty-acid and sterol biosynthesis [S. cerevisiae, YHR079C] 5e-06 [FUNCAT] 30.07 organization of endoplasmatic reticulum [S. cerevisiae, YHR079C] 5e-06 [FUNCAT] 30.01 organization of cell wall [S. cerevisiae, YIR019c] le-05 [FUNCAT] 30.90 extracellular/secretion proteins [S. cerevisiae, YIR019c] le-05 [FUNCAT] 01.05.01 carbohydrate utilization [S. cerevisiae, YIR019c] le-05 [FUNCAT] 04.05.01.01 general transcription activities [S. cerevisiae, YDL108w] le-05 [FUNCAT] 01.02.04 regulation of nitrogen and sulphur utilization [Ξ. cerevisiae, YNL183C] 8e -05 [FUNCAT] 08.99 other intracellular-transport activities [S. cerevisiae, YNL183c] 8e-05 [FUNCAT] 03.10 sporulation and germination [S. cerevisiae, YDR523C] 2e-04 [FUNCAT] c energy conversion [M. genitalium, MG109] 3e-04 [BLOCKS] BL00107A Protein kinases ATP-binding region proteins [BLOCKS] BL00939F [SCOP] dlgol 1 9 MAP kinase Erk2 [rat Rattus norvegicus 3e-62 [SCOP] dlwfc 1 8 MAP kinase p38 [human (Homo sapiens) 5e-59 [SCOP] dlkoa_2 1 7 (1-350) Twitchm, kinase domain [Caenorhabditi le-75 [SCOP] dlkoba_ 1 6 Twitchin, kinase domain [California sea har le-72 [SCOP] dlphk 1 5 gamma-subunit of glycogen phosphorylase kinas 4e-65 [SCOP] dlirk 2 4 insulin receptor [Human (Homo sapiens) 2e-56 [SCOP] dlapme_ 1 4 cAMP-dependent PK, catalytic subunit [mouse (Mu 4e-71 [SCOP] dlfgka_ 5.1.1 2 3 Fibroblast growth factor receptor 1 [human (Hom le-50 [SCOP] dlydre_ 5.1.1.1 3 cAMP-dependent PK, catalytic subunit [bovme (Bo 3e-70 [SCOP] dlfmk_3 5.1.1.2 2 (168-437) c-src tyrosine kinase [human (Hom 5e-49 [SCOP] dlcdkb_ 5.1.1.1.2 cAMP-dependent PK, catalytic subunit [pig (Su 2e-72 [SCOP] d2hcka3 5.1.1.2.1 (167-437) Haemopoetic cell kinase Hck [huma 5e-46 [SCOP] dlcsn 5.1.1.1.11 Casein kιnase-1, CK1 [Schizosaccharomyces pombe 9e-42 [SCOP] dljsua_ 5.1.1.1.1 Cyclin-dependent PK [Human (Homo sapiens) le-56 [SCOP] dlckιa_ 5.1.1.1.10 Casein kιnase-1, CK1 [rat (Rattus norvegicus) 9e-52 [EC] 2.7.1.38 Phosphorylase kinase 3e-29 [EC] 2.7.1.123 Ca2+/calmodulιn-dependent protein kinase 8e-66 [EC] 2.7.1.128 [Acetyl-CoA carboxylase] kinase 2e-17 [EC] 2.7.1.117 Myosm-light-chain kinase 2e-38 [EC] 2.7.1.109 [Hydroxymethylglutaryl-CoA reductase (NADPH) ] kinase 2e-17 [EC] 2.7.1.37 Protein kinase 6e-28 [PIRKW] phosphotransferase 8e-66 [PIRKW] nucleus 2e-24 [PIRKW] transferase 8e-30 [PIRKW] calcium 2e-27 [PIRKW] duplication 4e-19 [PIRKW] tandem repeat 2e-31 [PIRKW] phorbol ester binding le-16 [PIRKW] zinc le-16 [PIRKW] cell cycle control 2e-20 [PIRKW] serme/threonine-specific protein kinase 8e-66 [PIRKW] phospholipid binding le-16 [PIRKW] autophosphorylation 8e-66 [PIRKW] brain le-14 [PIRKW] heterotetramer 2e-16 [PIRKW] polymer 3e-29 [PIRKW] mitosis 2e-20 [PIRKW] magnesium le-22 [PIRKW] ATP 8e-66 [PIRKW] alternative initiators le-29 [PIRKW] phosphoprotem 8e-66 [PIRKW] apoptosis 2e-31 [PIRKW] glycoprotein 4e-19 [PIRKW] skeletal muscle 3e-28 [PIRKW] protein kinase 2e-28 [PIRKW] testis 3e-28 [PIRKW] signal transduction le-21 [PIRKW] cAMP binding le-16 [PIRKW] purine nucleotide binding 5e-25 [PIRKW] structural protein 4e-19 [PIRKW] calcium binding 3e-45 [PIRKW] alternative splicing 3e-45 [PIRKW] P-loop 5e-25 [PIRKW] lipoprotein 2e-16 [PIRKW] cardiac muscle 4e-19 [PIRKW] muscle 3e-28 [PIRKW] myristylation 2e-16 [PIRKW] EF hand 5e-29 [PIRKW] cell division 2e-38 [PIRKW] calmodulin binding 8e-66 [PIRKW] smooth muscle 7e-31 [SUPFAM] fibronectin type III repeat homology 7e-31 [SUPFAM] immunoglobulin homology 7e-31 [SUPFAM] ribosomal protein S6 kinase II 3e-26 [SUPFAM] calcium-dependent protein kinase 5e-29 [SUPFAM] AMP-activated protein k ase 7e-22 [SUPFAM] protein kinase akt le-14 [SUPFAM] protein kinase SPK1 3e-20 [SUPFAM] unassigned Ser/Thr or Tyr-specific protein kinases 2e-36 [SUPFAM] Ca2+/calmodulιn-dependent protein kmase 3e-45 [SUPFAM] calmodulin repeat homology 5e-29 [SUPFAM] protein kinase DUN1 2e-24 [SUPFAM] Dictyostelium cAMP-dependent protein kinase catalytic chain le-14 [SUPFAM] death-associated protein kmase 2e-31 [SUPFAM] myosm-light-chain kinase, nonmuscle le-29 [SUPFAM] pleckstπn repeat homology le-14 [SUPFAM] ankyrin repeat homology 2e-31 [SUPFAM] protein kinase homology 8e-66 [SUPFAM] Ca2+/calmodulm-dependent protein kinase II 8e-36 [SUPFAM] twitchin le-18 [SUPFAM] protein kinase C zinc-binding repeat homology le-16 [SUPFAM] titin 4e-19 [SUPFAM] protein kinase cdrl 2e-20 [SUPFAM] kinase-related transforming protein 2e-38 [SUPFAM] Ca2+/calmodulm-dependent protein kinase I 8e-66 [SUPFAM] kinase interaction domain homology 2e-24 [SUPFAM] protein kinase C mu le-16 [PROSITE] AMIDATION 1 [PROSITE] MYRISTYL 3 [PROSITE] CK2_PHOSPHO_SITE 10 [PROSITE] TYR_PHOSPHO_SITE 2 [PROSITE] PKC_PHOSPHO_SITE 11 [PFAM] Eukaryotic protein kinase domain [KW] All_Alpha [KW] 3D [KW] LOW COMPLEXITY 7.40 % SEQ MPFGCVTLGDKKNYNQPSEVTDRYDLGQVIKTEEFCEIFRAKDKTTGKLHTCKKFQKRDG SEG la06- CEETTTGGGCEEEEEECBCGGGGGEEEEEETTTTCEEEEEEEEC SEQ RKVRKAAKNEIGILKMVKHPNILQLVDVFVTRKEYFIFLELATGREVFDWILDQGYYSER SEG la06- HHHHHHHHHCCTTTBCCEEEEEEETTEEEEEECCCCCEEHHHHHHHTTTTBHH SEQ DTSNVVRQVLEAVAYLHSLKIVHRNLKLENLVYYNRLKNSKIVISDFHLAKLENGLIKEP SEG la06- HHHHHHHHHHHHHHHHHHHCCCTTTTTTTTEEECCCTTTTCEEECCCTTTTCHHHHHCCC SEQ CGTPEYLGNPPFYEEVEEDDYENHDKNLFRKILAGDYEFDSPYWDDISQAAKDLVTRLME SEG la06- HHHHHHHCCTTTTTT THHHHHHHHHCCCCCCTTTTTTTTCHHHHHHHHHHCT SEQ VEQDQRITAEEAISHEWISGNAASDKNIKDGVCAQIEKNFARAKWKKAVRVTTLMKRLRA SEG la06- TTGGGCCCHHHHHHTTTTTTCCCCCCBHHHHHHHHHHHHHCCTTTTTTBTHHHHHHHC .. SEQ PEQSSTAAAQSASATDTATPGAAGGATAAAASGATSAPEGDAARAAKΞDNVAPADRSATP SEG ..xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx la06- SEQ ATDGSATPATDGSVTPATDGSITPATDGSVTPATDRSATPATDGRATPATEESTVPTTQS SEG la06- SEQ SAMLATKAAATPEPAMAQPDSTAPEGATGQAPPSSKGEEAAGYAQESQREEAS SEG la06- Prosite for DKFZphfbr2_82e4.1 PΞ00005 21->24 PKC PHOSPHO SITE PDOC00005 PS00005 46->49 PKC PHOSPHO " -SITE PDOC00005 PS00005 51->54 PKC PHOSPHO " " SITE PDOC00005 PS00005 91->94 PKC PHOSPHO SITE PDOC00005 PS00005 103->106 PKC PHOSPHO SITE PDOC00005 PS00005 118->121 PKC PHOSPHO " " SITE PDOC00005 PS00005 138->141 PKC PHOSPHO- " SITE PDOC00005 PS00005 264->267 PKC PHOSPHO " " SITE PDOC00005 PS00005 394->397 PKC PHOSPHO " " SITE PDOC00005 PS00005 454->457 PKC PHOSPHO- SITE PDOC00005 PS00005 467->470 PKC PHOSPHO " " SITE PDOC00005 PS00006 7->ll CK2 PHOSPHO SITE PDOC00006 PS00006 91->95 CK2 PHOSPHO SITE PDOC00006 PS00006 103-M07 CK2 PHOSPHO SITE PDOC00006 PS00006 118->122 CK2 PHOSPHO " SITE PDOC00006 PS00006 248->252 CK2 PHOSPHO " SITE PDOC00006 PS00006 313->317 CK2 PHOSPHO " SITE PDOC00006 PS00006 336->340 CK2 PHOSPHO " SITE PDOC00006 PS00006 442->446 CK2 PHOSPHO " SITE PDOC00006 PΞ00006 455->459 CK2 PHOSPHO " SITE PDOC00006 PS00006 467->471 CK2 PHOSPHO " SITE PDOC00006 PS00007 456->464 TYR PHOSPHO " SITE PDOC00007 PS00007 1 2 7->136 TYR PHOSPHO " SITE PDOC00007 PS00008 260->266 MYRISTYL PDOC00008 PS00008 321->327 MYRISTYL PDOC00008 PS00008 324->330 MYRISTYL PDOC00008 PS00009 59->63 AMIDATION PDOC00009 Pfam for DKFZphfbr2_82e4.1 HMM_NAME Eukaryotic protein kinase domain HMM ► YeigRiIGeGsFGtVYkCiWr.TGelVAIKIIkkrsms FlREIq Y +G++I F ++++++++ TG++ K++ KR+ + +EI Query 24 YDLGQVIKTEEFCEIFRAKDKTTGKLHTCKKFQKRDGRKVRKAAKNEIG 72 HMM IMRrLnHPNIIRFYDwFedddDHIYMIMEYMeGGDLFDYIrrngpMsEwe I+++++HPNI+++ D+F + +++ + +E++ G + FD+I ++G++SE++ Query 73 ILKMVKHPNILQLVDVFV-TRKEYFIFLELATGREVFDWILDQGYYSERD 121 HMM IrflMyQILrGMeYLHSMgllHRDLKPENILIDeN...gqIKIcDFGLAR ++++Q+L++++YLHS +I+HR LK EN+ + ++ I I+DF LA+ Query 122 TSNVVRQVLEAVAYLHSLKIVHRNLKLENLVYYNRLKNSKIVISDFHLAK 171 HMM qMnnYerMttfCGTPWY* + N ++ + CGTP+Y Query 172 LEN—GLIKEPCGTPEY 186 HMM *GepPFyd dnMemlmrliqrfrrpfWpnCSeElyDFMr G PPFY+ + +++I++++++F +P+W+ +S ++D+++ Query ιε GNPPFYEEVEEDDYENHDKNLFRKILAGDYEFDSPYWDDISQAAKDLVT 236 HMM wCWnyDPekRPTFrQILnHPWF* +++++ ++R+T+++++ H W+ Query 237 RLMEVEQDQRITAEEAISHEWI 258 DKFZphfbr2_82gl4 group: transmembrane protein DKFZphfbr2_82gl4 encodes a novel 208 ammo acid proline-rich protein without similarity to known proteins. The protein contains one transmembrane domain. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of bram-specific genes and as a new marker for neuronal cells. unknown prolm rich protein membrane regions : 1 Summary DKFZphfbr2_82gl4 encodes a novel 208 ammo acid protein. unknown prolin rich protein complete cDNA, complete eds, EST hits TRANSMEMBRANE 1 Sequenced by DKFZ Locus: /map="26.2 cR from top of Chrl6 linkage group" Insert length: 2059 bp Poly A stretch at pos. 2049, polyadenylation signal at pos. 2024 1 AGAAGTGCGA CTGCCAGCTG CCGAGGCGTT CGGTCCTGCT GTTGCGGCCG 51 CTGCCCCAGG GCTGCGGGGA CGCTCCCGGA GCCCTGCCTG TCCCCTGTCC 101 ATCCAGGCCA GCAGCTGAAG GAGCCTCACC TGCCTCCCTT CTCTGAGTAG 151 CACGGATTTG AGGAGAAGCA GCGAAGATGT CCAGCGAGCC TCCCCCTCCT 201 TATCCTGGGG GCCCCACAGC CCCACTTCTG GAAGAGAAAA GTGGAGCCCC 251 GCCCACCCCA GGCCGTTCCT CCCCAGCTGT GATGCAGCCC CCTCCAGGCA 301 TGCCACTGCC CCCTGCGGAC ATTGGCCCCC CACCCTATGA GCCGCCGGGT 351 CACCCAATGC CCCAGCCTGG CTTCATCCCA CCACACATGA GTGCAGATGG 401 CACCTACATG CCTCCGGGTT TCTACCCTCC TCCAGGCCCC CACCCACCCA 451 TGGGCTACTA CCCCCCAGGG CCCTACACGC CAGGGCCCTA CCCTGGCCCT 501 GGGGGCCACA CAGCCACAGT CCTGGTCCCT TCAGGAGCTG CCACCACGGT 551 GACAGTGCTG CAGGGAGAGA TCTTTGAGGG AGCGCCTGTG CAGACGGTGT 601 GTCCCCACTG CCAGCAGGCC ATCGCCACCA AGATCTCCTA CGAGATTGGC 651 TTGATGAATT TCGTGCTGGG TTTCTTCTGT TGCTTCATGG GATGTGATCT 701 GGGCTGCTGC CTGATCCCCT GCCTCATCAA TGACTTCAAG GATGTGACGC 751 ACACATGCCC CAGCTGCAAA GCCTACATCT ACACGTACAA GCGCCTGTGC 801 TAACGGAGCT GGGACTCGGG ACTCCCCCGC CTGTCAGTCT GGCCCCCTGT 851 GCTTTGCTCC CTGCGCTCAG TGGTCACTTT CCCGCTCCCA CTTGGGGCTG 901 GGAGCCGTGC CACCATCCCC TAGAAGTCCT GTCCTCTTCA CCCTGCCCTA 951 CCTGAGCCGC TGACTCTTCT GGCAAAAATT CTGTTGGGAT TTAAGGCCAA 1001 GGGTCAGTGG GTGGCAGGGG GCTGGCAATG AGCTTGTGTG TTGTTGGTCT 1051 GCTTGGTGTG TGTGATCGGG AAGATAAGCT GGGAGGGGTC TCCTGCTGGG 1101 GTCCTGATGC CTCTGTTTCC AAACAAGGTA CAGGTTCAGT CCAGACTCTT 1151 TCCCCCTGGG ACCAACAGCA GCCAGAGCAG TTAGCCAGTT AGTCCCCAGG 1201 CCTGTGGCCA CAGGCGTTTC TGACCTGCTG GGCCGAGAAT GGGTAAGTTG 1251 TCTGGAGTCA GGTGGGCCCA CGTAGGACAG GGTCACAAAG CCTGGGTTTG 1301 TTTCTGGGTA CTTTGCGCCT CTGGGGTGCT AGAGGTGGGG CATGGTGGCT 1351 GGAAGTAAAA CTGCCAACTC TGGCCCTCAG AACTCTCAGG TATAGAAGCC 1401 CAGGATGTCT AATACCCTGT CCCAGTGCCC GAGAGCTGCC TGGTGTCAGG 1451 TAGAGAGGAC ACTGTACCTG GGTGAATGAT CAGACCCTGG TAGCTAAGAA 1501 GGAACTTGTC CCTTTGAGTC AGTGTGCAGA CCCCCTTTCA GGCCATGCCT 1551 CTGTGAACCC TGTATTGCTG GGGCCGGAAG GAGCCCCTGA GCCTAGCCCC 1601 TTCCCGTCTG CCCTGTGTCC TCACTGCGTG TGGGTATGAC CTCTGCCTGG 1651 TGGCTGGTGT ATCCCAACTG GGCAAGAGAT GGCAGAGGGT CCCCCTTGTG 1701 GGTGCGCTTG GATGTGCAGA GCCTTCTCCA TGGATTTTCT TCCCTGTAAG 1751 TGCCGGGCCC CCCACCCCAG CTGACAGGCT GTTGCTGTGC CTGCTCACAC 1801 CTGCTCCTGC AGGCACACTG GGCTAGGGAC GAGGAAGGAG CAGCCACAAG 1851 TGGTAGAACT GCCTTGGTGG ACACCAGCCT CGCCCTGTCT TTATTTCCTG 1901 AATGGTTTGT GAACTTGCTC ACCTGGACCA CTGTATCCTG CCACTGTCCT 1951 TCCTGGTCTC GCACTGCCAC TGCATGGCCT CCTGTCACTG TGAATCGTGG 2001 CCCAGTCTCA GTTTGTAGTT TCTCATTAAA TTGGCCCTTT CACTCCCCCA 2051 AAAAAAAAA BLAST Results Entry HS727347 from database EMBL: human STS WI-16589. Length = 275 Plus Strand HSPs: Score = 1365 (204.8 bits), Expect = 3.0e-55, P = 3.0e-55 Identities = 275/276 (99%), Positives = 275/276 (99%), Strand = Plus / PI Medline entries No Medlme entry Peptide information for frame 3 1 MSSEPPPPYP GGPTAPLLEE KSGAPPTPGR SSPAVMQPPP GMPLPPADIG 51 PPPYEPPGHP MPQPGFIPPH MSADGTYMPP GFYPPPGPHP PMGYYPPGPY 101 TPGPYPGPGG HTATVLVPSG AATTVTVLQG EIFEGAPVQT VCPHCQQAIA 151 TKISYEIGLM NFVLGFFCCF MGCDLGCCLI PCLINDFKDV THTCPSCKAY 201 IYTYKRLC ORF from 177 bp to 800 bp; peptide length: 208 Category: similarity to known protein BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_82gl4, frame 3 PIR:S57447 HPBRII-7 protein - human, N = 1, Score = 206, P = 8.4e-16 PIR:A47655 spliceosome-associated protein SAP 62 - human, N = 1, Score = 198, P = 4.3e-15 >PIR:S57447 HPBRII-7 protein - human Length = 551 HSPs: Score = 206 (30.9 bits), Expect = 8.4e-16, P = 8.4e-16 Identities = 57/115 (49%), Positives = 62/115 (53%) Query: 5 PPPPYPGGPTAPLLEEKSGAPPTPGRSSPAVMQPPPGMPLPPADIGPP PYEP 56 PPPP+P G T P G P PG P PPPG LPP GPP P P Sbjct: 226 PPPPFPAGQTPP--RPPLGPPGPPGPPGP PPPGQVLPPPLAGPPNRGDRPPPPVLF 279 Query: 57 PGHPMPQP—GFIPPHMSADGTYMP-PGFYPPPGPHPPM-GYYPP-GPYTPGPYPGPGGH 111 PG P QP G +PP G P PG+ PPPGP PP G PP GP+ P P PGP G Sbjct: 280 PGQPFGQPPLGPLPP GPPPPVPGYGPPPGPPPPQQGPPPPPGPFPPRP-PGPLGP 333 Query: 112 TATVLVP 118 T+ P Sbjct: 334 PLTLAPP 340 Score = 177 (26.6 bits), Expect = 1. le-12, P = 1. le-12 Identities = 55/120 (45%), Positives = 61/120 (50%) Query: 5 PPPPYPGGPTAP—LLEEKSGAPPTPG-RSSPAVM QP PPGMPLPPADIGPPPYE 55 P PP P GP P +L PP G R P V+ QP PP PLPP GPPP Sbjct: 244 PGPPGPPGPPPPGQVLPPPLAGPPNRGDRPPPPVLFPGQPFGQPPLGPLPP GPPP-P 299 Query: 56 PPGHPMPQPGFIPPHMSADGTYMPPGFYPP--PGP-HPPMGYYPPGPYTPGPYPG PG 109 PG+ P PG PP G PPG +PP PGP PP+ PP P+ PGP PG P Sbjct: 300 VPGYG-PPPGPPPPQQ GPPPPPGPFPPRPPGPLGPPLTLAPP-PHLPGPPPGAPPPA 354 Query: 110 GHTATVLVP 118 H P Sbjct: 355 PHVNPAFFP 363 Score = 168 (25.2 bits), Expect = 1. le-11, P = 1. le-11 Identities = 47/118 (39%), Positives = 51/118 (43%) Query: 5 PPPPYPG-GPTAPLLEEKSGAPPTPGRSSPAVMQP—PPGMPLPPADI-GPPPYEPPGHP 60 PPPP PG GP + G PP PG P P PP PP + GPPP PP P Sbjct: 296 PPPPVPGYGPPPGPPPPQQGPPPPPGPFPPRPPGPLGPPLTLAPPPHLPGPPPGAPPPAP 355 Query: 61 MPQPGFIPPHMSADGTYMPPGFYPPPGPHPPMGYYPPGPYTPGPYPGPGGHTATVLVPSG 120 P F PP ++ MP P P P G PP PY G Y PG T P Sbjct: 356 HVNPAFFPPPTNSG MPTSDSRGPPPTDPYGR-PP-PYDRGDYGPPGREMDTARTPLS 410 Query: 121 AA 122 A Sbjct: 411 EA 412 Score = 156 (23.4 bits), Expect = 2. le-10, P = 2. le-10 Identities = 44/103 (42%), Positives = 50/103 (48%) Query: 6 PPPYPGGPTAPLLEEKSGAPPT-PGRSSPAVMQPPPGMPLPPADIGPPPYEPPGHPMPQP 64 P PGG P G PP P +P +PP G P PP GPPP PG +P P Sbjct: 208 PGAVPGGDRFPGPAGPGGPPPPFPAGQTPP—RPPLGPPGPPGPPGPPP PGQVLPPP 262 Query: 65 GFIPPHMSADGTYMPPGFYP-PPGPHPPMGYYPPGPYTP GPYPGP 108 PP+ D PP +P P PP+G PPGP P GP PGP Sbjct: 263 LAGPPNRG-DRP-PPPVLFPGQPFGQPPLGPLPPGPPPPVPGYGPPPGP 309 Score = 121 (18.2 bits), Expect = 5.2e-05, P = 5.2e-05 Identities = 40/90 (44%), Positives = 45/90 (50%) Query: 23 GAPPTPGRSSPAVMQPP-PGMPLPPAD-IGPP-PYEPPGHPMPQPG-FIPPHMSADGTYM 78 G PG + P PP P PP +GPP P PPG P P PG +PP ++ Sbjct: 213 GGDRFPGPAGPGGPPPPFPAGQTPPRPPLGPPGPPGPPG-P-PPPGQVLPPPLAG 265 Query: 79 PP—GFYPPPG PHPPMGYYPPGPYTPGPYPG-PG 109 PP G PPP P P G P GP PGP P PG Sbjct: 266 PPNRGDRPPPPVLFPGQPFGQPPLGPLPPGPPPPVPG 302 Pedant information for DKFZphfbr2_82gl4, frame 3 Report for DKFZphfbr2_82gl4.3 [LENGTH] 208 [MW] 21862.47 [pi] 5.55 [PROSITE] MYRISTYL 3 [PROSITE] PKC_PHOSPHO_SITE 2 [KW] TRANSMEMBRANE 1 [KW] LOW_COMPLEXITY 39.90 % SEQ MSSEPPPPYPGGPTAPLLEEKSGAPPTPGRSSPAVMQPPPGMPLPPADIGPPPYEPPGHP SEG ....xxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD ccccccccccccccchhhhhhccccccccccccccccccccccccccccccccccccccc MEM SEQ MPQPGFIPPHMSADGTYMPPGFYPPPGPHPPMGYYPPGPYTPGPYPGPGGHTATVLVPSG SEG xxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD ccccccccccccccccccccccccccccccccccccccccccccccccccceeeeecccc MEM SEQ AATTVTVLQGEIFEGAPVQTVCPHCQQAIATKISYEIGLMNFVLGFFCCFMGCDLGCCLI SEG PRD cceeeeeeeeeeecccceeeeccchhhhhhhhhhhhhhhceeeeeeeeeecccccceeec MEM MMMMMMMMMMMMM SEQ PCLINDFKDVTHTCPSCKAYIYTYKRLC SEG . PRD eeeecccccccccccccceeeeeeeccc MEM MMMM Prosite for DKFZphfbr2 82gl4.3 PS00005 196->199 PKC_PHOSPHO_SITE PDOC00005 PS00005 203->206 PKC_PHOSPHO_SITE PDOC00005 PS00008 109->115 MYRISTYL PDOC00008 PS00008 120->126 MYRISTYL PDOC00008 PΞ00008 172->178 MYRISTYL PDOC00008 (No Pfam data available for DKFZphfbr2_82gl4.3) DKFZphfbr2_82ιl7 group: signal transduction DKFZphtes2_82ιl7 encodes a novel 334 ammo acid protein with similarity to the plasma membrane substrate for the cAMP-dependent protein kinase. The novel protein s a transmembrane protein with strong similarity to the phospholemman protein, a membrane substrate for the cAMP-dependent protein kinase. It seems to serve as a chloride channel or as a chloride-channel regulator. The new protein can find application in modulating/blocking cAMP-dependent protein kinase- dependent pathways . similarity to plasma membrane substrate for cAMP-dependent protein kinase complete cDNA, complete eds, EST hits potential start at Bp 31 matches Kozak consensus PyNNatgG might be a SODIUM/POTASSIUM-TRANSPORTING ATPASE TRANSMEMBRANE 1 Sequenced by DKFZ Locus: /map="ll; 920_E_12; 786_(A, H)_ll; (797, 802)_(E, H)_7" Insert length: 1647 bp Poly A stretch at pos. 1637, polyadenylation signal at pos. 1615 1 AGTCTCGGAG GGGACCGGCT GTGCAGACGC CATGGAGTTG GTGCTGGTCT 51 TCCTCTGCAG CCTGCTGGCC CCCATGGTCC TGGCCAGTGC AGCTGAAAAG 101 GAGAAGGAAA TGGACCCTTT TCATTATGAT TACCAGACCC TGAGGATTGG 151 GGGACTGGTG TTCGCTGTGG TTCTCTTCTC GGTTGGGATC CTCCTTATCC 201 TAAGTCGCAG GTGCAAGTGC AGTTTCAATC AGAAGCCCCG GGCCCCAGGA 251 GATGAGGAAG CCCAGGTGGA GAACCTCATC ACCGCCAATG CAACAGAGCC 301 CCAGAAAGCA GAGAACTGAA GTGCAGCCAT CAGGTGGAAG CCTCTGGAAC 351 CTGAGGCGGC TGCTTGAACC TTTGGATGCA AATGTCGATG CTTAAGAAAA 401 CCGGCCACTT CAGCAACAGC CCTTTCCCCA GGAGAAGCCA AGAACTTGTG 451 TGTCCCCCAC CCTATCCCCT CTAACACCAT TCCTCCACCT GATGATGCAA 501 CTAACACTTG CCTCCCCGCT GCAGCCTGTG GTCCTGCCCA CCTCCCGTGA 551 TGTGTGTGTG TGTGTGTGTG TGTGTGACTG TGTGTGTTTG CTAACTGTGG 601 TCTTTGTGGC TACTTGTTTG TGGATGGTAT TGTGTTTGTT AGTGAACTGT 651 GGACTCGCTT TCCCAGGCAG GGGCTGAGCC ACACGGCCAT CTGCTCCTCC 701 CTGCCCCCGT GGCCCTCCAT CACCTTCTGC TCCTAGGAGG CTGCTTGTTG 751 CCCGAGACCA GCCCCCTCCC CTGATTTAGG GATGCGTAGG GTAAGAGCAC 801 GGGCAGTGGT CTTCAGTCGT CTTGGGACCT GGGAAGGTTT GCAGCACTTT 851 GTCATCATTC TTCATGGACT CCTTTCACTC CTTTAACAAA AACCTTGCTT 901 CCTTATCCCA CCTGATCCCA GTCTGAAGGT CTCTTAGCAA CTGGAGATAC 951 AAAGCAAGGA GCTGGTGAGC CCAGCGTTGA CGTCAGGCAG GCTATGCCCT 1001 TCCGTGGTTA ATTTCTTCCC AGGGGCTTCC ACGAGGAGTC CCCATCTGCC 1051 CCGCCCCTTC ACAGAGCGCC CGGGGATTCC AGGCCCAGGG CTTCTACTCT 1101 GCCCCTGGGG AATGTGTCCC CTGCATATCT TCTCAGCAAT AACTCCATGG 1151 GCTCTGGGAC CCTACCCCTT CCAACCTTCC CTGCTTCTGA GACTTCAATC 1201 TACAGCCCAG CTCATCCAGA TGCAGACTAC AGTCCCTGCA ATTGGGTCTC 1251 TGGCAGGCAA TAGTTGAAGG ACTTCCTGTT CCGTTGGGGC CAGCACACCG 1301 GGATGGATGG AGGGAGAGCA GAGGCCTTTG CTTCTCTGCC TACGTCCCCT 1351 TAGATGGGCA GCAGAGGCAA CTCCCGCATC CTTTGCTCTG CCTGTCAGTG 1401 GTCAGAGCGG TGAGCGAGGT GGGTTGGAGA CTCAGCAGGC TCCGTGCAGC 1451 CCTTGGGAAC AGTGAGAGGT TGAAGGTCAT AACGAGAGTG GGAACTCAAC 1501 CCAGATCCCG CCCCTCCTGT CCTCTGTGTT CCCGCGGAAA CCAACCAAAC 1551 CGTGCGCTGT GACCCATTGC TGTTCTCTGT ATCGTGACCT ATCCTCAACA 1601 ACAACAGAAA AAAGGAATAA AATATCCTTT GTTTCCTAAA AAAAAAA BLAST Results Entry HS31455 from database EMBL: human STS WI-2739. Length = 103 Minus Strand HSPs: Score = 487 (73.1 bits), Expect = 4.4e-14, P = 4.4e-14 Identities = 101/104 (97%), Positives = 101/104 (97%), Strand = Minus / Plus frame shift in primer binding site Medlme entries 91250422: Purification and complete sequence determination of the major plasma membrane substrate for cAMP-dependent protein kinase and protein kinase C in myocardium. 95091702: Protein kinase C and cyclic AMP-dependent protein kinase phosphorylate phospholemman, an insulin and adrenaline-regulated membrane phosphoprotem, at specific sites in the carboxy terminal domain. 95138184: Mat-8, a novel phospholemman-like protein expressed in human breast tumors, induces a chloride conductance in Xenopus oocytes. Peptide information for frame 2 1 MELVLVFLCS LLAPMVLASA AEKEKEMDPF HYDYQTLRIG GLVFAVVLFS 51 VGILLILSRR CKCSFNQKPR APGDEEAQVE NLITANATEP QKAEN ORF from 32 bp to 316 bp; peptide length: 95 Category: strong similarity to known protein BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_82ιl7, frame 2 SWISSPROT :PLM_HUMAN PHOSPHOLEMMAN PRECURSOR., N = 1, Score = 196, P = 1.2e-15 TREMBL: AF091390_1 product: "phospholemman precursor"; Mus musculus phospholemman precursor, gene, complete eds., N = 1, Score = 187, P = 1. le-14 PIR:A40533 cAMP-dependent protein kinase major membrane substrate precursor - dog, N = 1, Score = 189, P = 6.5e-15 SWISSPROT :PLM_RAT PHOSPHOLEMMAN PRECURSOR., N = 1, Score = 185, P = 1.7e-14 >SWISSPROT:PLM_HUMAN PHOSPHOLEMMAN PRECURSOR. Length = 92 HSPs: Score = 196 (29.4 bits), Expect = 1.2e-15, P = 1.2e-15 Identities = 43/85 (50%), Positives = 56/85 (65%) Query: 4 VLVFLCSLLAPMVLASAAEKEKEMDPFHYDYQTLRIGGLVFAVVLFSVGILLILSRRCKC 63 +LVF LL + AE KE DPF YDYQ+L+IGGLV A +LF +GIL++LSRRC+C Sbjct: 7 ILVFCVGLLT MAKAESPKEHDPFTYDYQSLQIGGLVIAGILFILGILIVLSRRCRC 62 Query: 64 SFNQKPRA--PGDEEAQVENLITANAT 88 FNQ+ R P +EE + 1 +T Sbjct: 63 KFNQQQRTGEPDEEEGTFRSSIRRLST 89 Pedant information for DKFZphfbr2_82ιl7, frame 2 Report for DKFZphfbr2_82ιl7.2 [LENGTH] 95 [MW] 10542.37 [pi] 5.05 [HOMOL] SWISSPROT: PLM_HUMAN PHOSPHOLEMMAN PRECURSOR. 3e-15 [BLOCKS] BL01310 [EC] 3.6.1.37 Na+/K+-exchangmg ATPase 6e-08 [PIRKW] transmembrane protein le-09 [PIRKW] hydrolase 6e-08 [PROSITE] ATP1G1_PLM_MAT8 1 [PROSITE] MYRISTYL 1 [PROSITE] CK2_PHOSPHO_SITE 1 [PROSITE] TYR_PHOSPHO_SITE 1 [PROSITE] PKC_PHOSPHO_SITE 2 [PROSITE] ASN_GLYCOSYLATION 1 [KW] Alpha_Beta [KW] SIGNAL PEPTIDE 19 SEQ MELVLVFLCSLLAPMVLASAAEKEKEMDPFHYDYQTLRIGGLVFAWLFSVGILLILSRR PRD ccchhhhhhhhhhccccccccccccccccccceeeeecccceeeehhhhhhheeeeehhh SEQ CKCSFNQKPRAPGDEEAQVENLITANATEPQKAEN PRD hhhcccccccccccchhhhhhhhhhhccccccccc Prosite for DKFZphfbr2_82ιl7.2 PS00001 86->90 ASN_GLYCOSYLATION PDOC00001 PS00005 36->39 PKC_PHOSPHO_SITE PDOC00005 PS00005 58->61 PKC_PHOSPHO_SITE PDOC00005 PS00006 19->23 CK2_PHOSPHO_SITE PDOC00006 PS00007 25->33 TYR_PHOSPHO_SITE PDOC00007 PS00008 41->47 MYRISTYL PDOC00008 PS01310 28->42 ATP1G1 PLM MAT8 PDOC01014 (No Pfam data available for DKFZphfbr2_82ιl7.2) DKFZphfbr2_82ι24 group: nucleic acid management DKFZphfbr2_82ι24 encodes a novel 547 ammo acid protein with similarity to DEAD-box superfamily ATP-dependent helicases. RNA helicases comprise a large family of proteins that are involved in basic biological systems such as nuclear and mitochondrial splicing processes, RNA editing, rRNA processing, translation initiation, nuclear mRNA export, and mRNA degradation. RNA helicases are essential factors m cell development and differentiation, and some of them play a role in transcription and replication of viral single-stranded RNA genomes. The members of the largest subgroup, the DEAD and DEAH box proteins, exhibit a strong dependence of the unwinding activity on ATP hydrolysis . The novel protein contains a DEAD-box an ATP/GTP-b dmg site motif A (P-loop, interacting with one of the phophate groups of the nucleotide) and a leucine zipper. Mutations in the closely related Drosophila Hlc gene result in lethality in homozygotes. Therefore the new protein seems to be critical involved in RNA processing in eukaπontic c ells. The new protein can find application in modulating RNA metabolism and gene expression. strong similarity to DEAD-box subfamily ATP-dependent helicase complete cDNA, complete eds, EST hits potential Start at Bp 9 matches Kozak consensus PyNNatgG, [PFAM] Helicases conserved C-terminal domain [PFAM] DEAD and DEAH box helicases Sequenced by DKFZ Locus: /map="720_A_3; 758_H_4; 772_E_3; 804_A_5; 175.5 cR from topFT of Chr7 linkage group" Insert length: 1860 bp Poly A stretch at pos. 1850, polyadenylation signal at pos. 1829 1 AGCAGCGCCA TGGAGGACTC TGAAGCACTG GGCTTCGAAC ACATGGGCCT 51 CGATCCCCGG CTCCTTCAGG CTGTCACCGA TCTGGGCTGG TCGCGACCTA 101 CGCTGATCCA GGAGAAGGCC ATCCCACTGG CCCTAGAAGG GAAGGACCTC 151 CTGGCTCGGG CCCGCACGGG CTCCGGGAAG ACGGCCGCTT ATGCTATTCC 201 GATGCTGCAG CTGTTGCTCC ATAGGAAGGC GACAGGTCCG GTGGTAGAAC 251 AGGCAGTGAG AGGCCTTGTT CTTGTTCCTA CCAAGGAGCT GGCACGGCAA 301 GCACAGTCCA TGATTCAGCA GCTGGCTACC TACTGTGCTC GGGATGTCCG 351 AGTGGCCAAT GTCTCAGCTG CTGAAGACTC AGTCTCTCAG AGAGCTGTGC 401 TGATGGAGAA GCCAGATGTG GTAGTAGGGA CCCCATCTCG CATATTAAGC 451 CACTTGCAGC AAGACAGCCT GAAACTTCGT GACTCCCTGG AGCTTTTGGT 501 GGTGGACGAA GCTGACCTTC TTTTTTCCTT TGGCTTTGAA GAAGAGCTCA 551 AGAGTCTCCT CTGTCACTTG CCCCGGATTT ACCAGGCTTT TCTCATGTCA 601 GCTACTTTTA ACGAGGACGT ACAAGCACTC AAGGAGCTGA TATTACATAA 651 CCCGGTTACC CTTAAGTTAC AGGAGTCCCA GCTGCCTGGG CCAGACCAGT 701 TACAGCAGTT TCAGGTGGTC TGTGAGACTG AGGAAGACAA ATTCCTCCTG 751 CTGTATGCCC TGCTCAAGCT GTCATTGATT CGGGGCAAGT CTCTGCTCTT 801 TGTCAACACT CTAGAACGGA GTTACCGGCT ACGCCTGTTC TTGGAACAGT 851 TCAGCATCCC CACCTGTGTG CTCAATGGAG AGCTTCCACT GCGCTCCAGG 901 TGCCACATCA TCTCACAGTT CAACCAAGGC TTCTACGACT GTGTCATAGC 951 AACTGATGCT GAAGTCCTGG GGGCCCCAGT CAAGGGCAAG CGTCGGGGCC 1001 GAGGGCCCAA AGGGGACAAG GCCTCTGATC CGGAAGCAGG TGTGGCCCGG 1051 GGCATAGACT TCCACCATGT GTCTGCTGTG CTCAACTTTG ATCTTCCCCC 1101 AACCCCTGAG GCCTACATCC ATCGAGCTGG CAGGACAGCA CGCGCTAACA 1151 ACCCAGGCAT AGTCTTAACC TTTGTGCTTC CCACGGAGCA GTTCCACTTA 1201 GGCAAGATTG AGGAGCTTCT CAGTGGAGAG AACAGGGGCC CCATTCTGCT 1251 CCCCTACCAG TTCCGGATGG AGGAGATCGA GGGCTTCCGC TATCGCTGCA 1301 GGGATGCCAT GCGCTCAGTG ACTAAGCAGG CCATTCGGGA GGCAAGATTG 1351 AAGGAGATCA AGGAAGAGCT TCTGCATTCT GAGAAGCTTA AGACATACTT 1401 TGAAGACAAC CCTAGGGACC TCCAGCTGCT GCGGCATGAC CTACCTTTGC 1451 ACCCCGCAGT GGTGAAGCCC CACCTGGGCC ATGTTCCTGA CTACCTGGTT 1501 CCTCCTGCTC TCCGTGGCCT GGTACGCCCT CACAAGAAGC GGAAGAAGCT 1551 GTCTTCCTCT TGTAGGAAGG CCAAGAGAGC AAAGTCCCAG AACCCACTGC 1601 GCAGCTTCAA GCACAAAGGA AAGAAATTCA GACCCACAGC CAAGCCCTCC 1651 TGAGGTTGTT GGGCCTCTCT GGAGCTGAGC ACATTGTGGA GCACAGGCTT 1701 ACACCCTTCG TGGACAGGCG AGGCTCTGGT GCTTACTGCA CAGCCTGAAC 1751 AGACAGTTCT GGGGCCGGCA GTGCTGGGCC CTTTAGCTCC TTGGCACTTC 1801 CAAGCTGGCA TCTTGCCCCT TGACAACAGA ATAAAAATTT TAGCTGCCCC 1851 AAAAAAAAAA BLAST Results Entry HSG05793 from database EMBL: human STS WI-6581. Length = 206 Minus Strand HSPs: Score = 992 (148.8 bits), Expect = 6.0e-38, P = 6.0e-38 Identities = 204/208 (98%), Positives = 204/208 (98%), Strand = Minus / PI Entry AC004938 from database EMBL: Homo sapiens clone DJ0971C03; HTGS phase 1, 18 unordered pieces. Score = 1269, P = 6.5e-202, identities = 269/282 12 exons Bp -87920-93706 (matching 1-1497) Medline entries No Medlme entry Peptide information for frame 1 ORF from 10 bp to 1650 bp; peptide length: 547 Category: strong similarity to known protein Classification: Nucleic acid management Prosite motifs: ATP_GTP_A (51-59) LEUCINE ZIPPER (149-171) 1 MEDSEALGFE HMGLDPRLLQ AVTDLGWSRP TLIQEKAIPL ALEGKDLLAR 51 ARTGSGKTAA YAIPMLQLLL HRKATGPVVE QAVRGLVLVP TKELARQAQS 101 MIQQLATYCA RDVRVANVSA AEDSVSQRAV LMEKPDVVVG TPSRILSHLQ 151 QDSLKLRDSL ELLVVDEADL LFSFGFEEEL KSLLCHLPRI YQAFLMSATF 201 NEDVQALKEL ILHNPVTLKL QESQLPGPDQ LQQFQVVCET EEDKFLLLYA 251 LLKLSLIRGK SLLFVNTLER SYRLRLFLEQ FSIPTCVLNG ELPLRSRCHI 301 ISQFNQGFYD CVIATDAEVL GAPVKGKRRG RGPKGDKASD PEAGVARGID 351 FHHVSAVLNF DLPPTPEAYI HRAGRTARAN NPGIVLTFVL PTEQFHLGKI 401 EELLSGENRG PILLPYQFRM EEIEGFRYRC RDAMRSVTKQ AIREARLKEI 451 KEELLHSEKL KTYFEDNPRD LQLLRHDLPL HPAVVKPHLG HVPDYLVPPA 501 LRGLVRPHKK RKKLSSSCRK AKRAKSQNPL RSFKHKGKKF RPTAKPS BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfbr2_82ι24, frame 1 TREMBL:AF017777_10 gene: "hlc"; product: "helicase"; Drosophila melanogaster tweety (tty) , flightless (fli), dodo (dod) , penguin (pen), small optic lobes (sol), innocent bystander (iby), waclaw (waw), bobby sox (bbx) , sluggish (slg) , helicase (hlc) , misato (mst) , and la costa (lcs) genes, complete eds., N = 1, Score = 1230, P = 3.2e-125 TREMBL:SPCC1494_6 gene: "SPCC1494.06c"; product: "atp dependent helicase"; S. pombe chromosome II cosmid C1494., N = 2, Score = 753, P = 2.5e-113 PIR:S51412 hypothetical protein YLR276c - yeast (Saccharomyces cerevisiae), N = 2, Score = 711, P = 8.2e-117 TREMBL :AF025451_2 gene: "C24H12.4"; Caenorhabditis elegans cosmid C24H12., N = 2, Score = 564, P = 2.7e-99 >TREMBL:AF017777_10 gene: "hlc"; product: "helicase"; Drosophila melanogaster tweety (tty), flightless (fli), dodo (dod), penguin (pen), small optic lobes (sol), innocent bystander (iby), waclaw (waw), bobby sox (bbx), sluggish (slg), helicase (hlc), misato (mst), and la costa (lcs) genes, complete eds. Length = 560 HSPs: Score = 1230 (184.5 bits), Expect = 3.2e-125, P = 3.2e-125 Identities = 251/497 (50%), Positives = 344/497 (69%) Query: 9 FEHMGLDPRLLQAVTDLGWSRPTLIQEKAIPLALEGKDLLARARTGSGKTAAYAIPMLQL 68 F + LD R+L+AV LGW +PTLIQ AIPL LEGKD++ RARTGSGKTA YA+P++Q Sb j ct: 11 FHELELDQRILKAVAQLGWQQPTLIQSTAIPLLLEGKDVVVRARTGSGKTATYALPLIQK 70 Query: 69 LLHRKATGPVVEQAVRGLVLVPTKELARQAQSMIQQLATYCARDVRVANVS-AAEDΞVSQ 127 +L+ K EQ V +VL PTKEL RQ++ +I+QL C + VRVA+++ ++ D+V+Q Sbjct: 71 ILNSKLNAS—EQYVSAVVLAPTKELCRQSRKVIEQLVESCGK VRVADIADSSNDTVTQ 128 Query: 128 RAVLMEKPDVVVGTPSRILSHLQQDSLKLRDSLELLVVDEADLLFSFGFEEELKSLLCHL 187 R L E PD+VV TP+ +L++ + S+ +E LVVDEADL+F++G+E++ K L+ HL Sbjct: 129 RHALSESPDIVVATPANLLAYAEAGSVVDLKHVETLVVDEADLVFAYGYEKDFKRLIKHL 188 Query: 188 PRIYQAFLMSATFNEDVQALKELILHNPVTLKLQESQLPGPDQLQQFQVVCETEEDKFLL 247 P IYQA L+SAT +DV +K L L+NPVTLKL+E +L DQL +++ E E DK + Sbjct: 189 PPIYQAVLVSATLTDDVVRMKGLCLNNPVTLKLEEPELVPQDQLSHQRILAE-ENDKPAI 247 Query: 248 LYALLKLSLIRGKSLLFVNTLERSYRLRLFLEQFSIPTCVLNGELPLRSRCHIISQFNQG 307 LYALLKL LIRGKS++FVN+++R Y++RLFLEQF I CVLN ELP R H ISQFN+G Sbjct: 248 LYALLKLRLIRGKSIIFVNSIDRCYKVRLFLEQFGIRACVLNSELPANIRIHTISQFNKG 307 Query: 308 FYDCVIATDAEVLGAPVKGKRRGRGPKGDKASDPEAGVARGIDFHHVSAVLNFDLPPTPE 367 YD +IA+D + P G + K ++ D E+ +RGIDF V+ V+NFD P Sbjct: 308 TYDIIIASDEHHMEKP—GGKSATNRKSPRSGDMESSASRGIDFQCVNNVINFDFPRDVT 365 Query: 368 AYIHRAGRTARANNPGIVLTFVLPTEQFHLGKIEELL SGENRGPILLPYQFRMEEI 423 +YIHRAGRTAR NN G VL+FV E +E+ L + + 1+ YQF+MEE+ Sbjct: 366 SYIHRAGRTARGNNKGSVLΞFVSMKESKVNDSVEKKLCDSFAAQEGEQIIKNYQFKMEEV 425 Query: 424 EGFRYRCRDAMRSVTKQAIREARLKEIKEELLHSEKLKTYFEDNPRDLQLLRHDLPLHPA 483 E FRYR +D R+ T+ A+ + R++EIK E+L+ EKLK +FE+N RDLQ LRHD PL Sbjct: 426 ESFRYRAQDCWRAATRVAVHDTRIREIKIEILNCEKLKAFFEENKRDLQALRHDKPLRAI 485 Query: 484 VVKPHLGHVPDYLVPPALRGLV 505 V+ HL +P+Y+VP AL+ +V Sbjct: 486 KVQSHLSDMPEYIVPKALKRVV 507 Pedant information for DKFZphfbr2_82ι24, frame 1 Report for DKFZphfbr2_82ι24.1 [LENGTH] 547 [MW] 61589.88 [pi] 9.34 [HOMOL] TREMBL:AF017777_10 gene: "hlc"; product: "helicase"; Drosophila melanogaster tweety (tty) flightless (fli), dodo (dod), penguin (pen), small optic lobes (sol), innocent bystander (ι:by), waclaw (waw), bobby sox (bbx), sluggish (slg), helicase (hlc), misato (mst), and la costa (lcs) genes, complete eds. le-121 [FUNCAT] 98 classification not yet clear-cut [S. cerevisiae, YLR276c] le-109 [FUNCAT] mrna translation and ribosome biogenesis [H. influenzae, HI0231 RNA] 2e-42 [FUNCAT] 04.01.04 rrna processing [S. cerevisiae, YLL008w] 8e-40 [FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YLL008w] 8e-40 [FUNCAT) 30.10 nuclear organization [S. cerevisiae, YLL008w] 8e-40 [FUNCAT] 05.04 translation (initiation, elongation and termination) [ S . cerevisiae YKR059w] 3e-39 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YKR059w ] 3e-39 [FUNCAT] 04.99 other transcription activities [S. cerevisiae, YDL160c ] 3e-35 [FUNCAT] 04.05.03 mrna processing (splicing) [S. cerevisiae, YPL119c ] 3e-29 [FUNCAT] 04.05.01.07 chromatin modification [S. cerevisiae, YMR290c ] 4e-29 [FUNCAT] 1 genome replication, transcription, recombination and repair [H. influenzae HI0892] le-27 [FUNCAT] 09.01 biogenesis of cell wall [S. cerevisiae, YJL033w] 2e-27 [FUNCAT] 30.16 mitochondrial organization [S. cerevisiae, YDR194c] 4e-21 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YGL064C] le-05 [BLOCKS] BL00039D DEAD-box subfamily ATP-dependent helicases proteins [BLOCKS] BL00039C DEAD-box subfamily ATP-dependent helicases proteins [BLOCKS] BL00039B DEAD-box subfamily ATP-dependent helicases proteins [BLOCKS] BL00039A DEAD-box subfamily ATP-dependent helicases proteins [PIRKW] nucleus 4e-34 [PIRKW] RNA binding 7e-41 [PIRKW] DEAD box 2e-38 [PIRKW] transmembrane protein 9e-20 [PIRKW] DNA binding 8e-23 [PIRKW] ATP le-107 [PIRKW] purine nucleotide binding 2e-38 [PIRKW] P-loop le-107 [PIRKW] hydrolase 2e-35 [PIRKW] protein biosynthesis 2e-38 [PIRKW] ATP binding 7e-43 [SUPFAM] WW repeat homology le-26 [SUPFAM] DEAD/H box helicase homology le-107 [SUPFAM] unassigned DEAD/H box helicases le-107 [SUPFAM] ATP-dependent RNA helicase DBP1 3e-31 [SUPFAM] ATP-dependent RNA helicase DHH1 2e-35 [SUPFAM] translation initiation factor eIF-4A 2e-38 [SUPFAM] tobacco ATP-dependent RNA helicase DB10 le-26 [PROSITE] ATP_GTP_A 1 [PROSITE] LEUCINE_ZIPPER 1 [PFAM] Helicases conserved C-termmal domain [PFAM] DEAD and DEAH box helicases [KW] Alpha_Beta [KW] LOW COMPLEXITY 9.87 % SEQ MEDSEALGFEHMGLDPRLLQAVTDLGWSRPTLIQEKAIPLALEGKDLLARARTGSGKTAA SEG PRD ccccccccccccccchhhhhhhhhhccccccccccccccccccccceeeeecccccccee SEQ YAIPMLQLLLHRKATGPVVEQAVRGLVLVPTKELARQAQSMIQQLATYCARDVRVANVSA SEG PRD ehhhhhhhhhhhcccccccccceeeeeeccchhhhhhhhhhhhhhhhhhhcceeeeeecc SEQ AEDSVSQRAVLMEKPDVVVGTPSRILSHLQQDSLKLRDSLELLVVDEADLLFSFGFEEEL SEG xxxxxxxxxxxx PRD ccchhhhhhhhhcccceeeeccccchhhhhhcccccchhhhhhhhhhhhhhhhhcchhhh SEQ KSLLCHLPRIYQAFLMSATFNEDVQALKELILHNPVTLKLQESQLPGPDQLQQFQVVCET SEG PRD hhhhhhccchhhhhhhhhccchhhhhhhhhhhcccceeeeeccccccchhhhhhhhhhhh SEQ EEDKFLLLYALLKLSLIRGKSLLFVNTLERSYRLRLFLEQFSIPTCVLNGELPLRSRCHI SEG xxxxxxxxxxx PRD hhhhhhhhhhhhhhhhccceeeeeeehhhhhhhhhhhhhhcccceeeccccchhhhhhhh SEQ ISQFNQGFYDCVIATDAEVLGAPVKGKRRGRGPKGDKASDPEAGVARGIDFHHVSAVLNF SEG xxxxxxxxxxxxx PRD hhhhhccceeeeeeccccccccccccccccccccccccccccccccccccccceeeeeec SEQ DLPPTPEAYIHRAGRTARANNPGIVLTFVLPTEQFHLGKIEELLSGENRGPILLPYQFRM SEG PRD ccccccceeeeccccccccccccceeeeeecchhhhhhhhhhhhhhhccccccccccchh SEQ EEIEGFRYRCRDAMRSVTKQAIREARLKEIKEELLHSEKLKTYFEDNPRDLQLLRHDLPL SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccchhhhhhhhccc SEQ HPAVVKPHLGHVPDYLVPPALRGLVRPHKKRKKLSSSCRKAKRAKSQNPLRSFKHKGKKF SEG xxxxxxxxxxxxxxxxxx PRD cccccccccccccceeeccccccccccccccccccchhhhhhcccccccccccccccccc SEQ RPTAKPS SEG PRD ccccccc Prosite for DKFZphfbr2_82ι24.1 PS00017 51->59 ATP_GTP_A PDOC00017 PS00029 149->171 LEUCINE ZIPPER PDOC00029 Pfam for DKFZphfbr2_82ι24.1 HMM_NAME DEAD and DEAH box helicases HMM *gLpPWILRnIyeMGFEkPTPIQQqAIPιILeGRDVMACAQTGSGKTAAF GL+P +L +++++G+++PT IQ++AIP++LEG+D++A+A TGSGKTAA+ Query 13 GLDPRLLQAVTDLGWSRPTLIQEKAIPLALEGKDLLARARTGSGKTAAY 61 HMM HPMLQHIDwdP... WpqpPQdPrALILAPTRELAMQIQEEcRkFgkHMn +IPMLQ +++ + + + +R+L+L+PT ELA+Q Q +++++ ++ Query 62 AIPMLQLLLHRKATGPVVEQA-VRGLVLVPTKELARQAQSMIQQLATYCA 110 HMM g.IRImcIYGGtnMRdQMRmLeRGpPHIVIATPGRLIDHIERgtldLDr. +R++ + + Q +L+++P ++V++TP R++ H+++ +L+L++ Query 111 RDVRVANVSAAEDSVSQRAVLMEKP-DVVVGTPSRILSHLQQDSLKLRDS 159 HMM IeMLVMDEADRMLDMGFIDQIRrlMrqlPMpwNRQTMMFSATMPdelqEL +E LV DEAD +++ GF++++ ++ ++P + Q + SAT+ +++Q L Query 160 LELLVVDEADLLFSFGFEEELKSLLCHLP—RIYQAFLMSATFNEDVQAL 207 HMM ARrFMRNPIRInldMdElTtnEnlkQwYiyVerEMWKfdcLcrLIe* + +++NP+ + + +++L + ++Q+ +++E E++KF +L+ L++ Query 208 KELILHNPVTLKLQESQLPGPDQLQQFQVVCETEEDKFLLLYALLK 253 HMM_NAME Helicases conserved C-terminal domain HMM *EιleeWLknlGIrvmYIHGdMpQeERdeIMddFNnGEynVLIcTDV... +L+ +L++ I+++++ G +P + R 1+ +FN+G Y++ I+TD+ Query 272 YRLRLFLEQFSIPTCVLNGELPLRSRCHIISQFNQGFYDCVIATDAEVL 320 HMM ggRGIDIPdVNHVINYDMPWNPEqYI +RGID+ V+ V N+D+P +PE YI Query 321 GAPVKGKRRGRGPKGDKASDPEAGVARGIDFHHVSAVLNFDLPPTPEAYI 370 HMM QRIGRTgRIG* +R+GRT+R++ Query 371 HRAGRTARAN 380 DKFZphfbr2_82ml6 group: brain derived DKFZphfbr2_82ml6 encodes a novel 289 amino acid protein with very weak similarity to A. haliana F28A23. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of brain-specific genes . similarity to A. thaliana F28A23.140 complete cDNA, complete eds, few EST hits many ATGs in front of the ORF TRANSMEMBRANE 1 Sequenced by DKFZ Locus: /map="4" Insert length: 2715 bp Poly A stretch at pos. 2705, polyadenylation signal at pos. 2687 1 AGAGGAGGGG AGAGGACTGG GGAGCCGAGC CAGAGCCGGG CTGCCTGCCA 51 CCCGGCTGCT CGTCCGCTAG CTGGGGAGGA GCGCTCCACC CGCAACTGAC 101 AAAGGATGGG AGAATGCCCG CGCCCCGGGA TGCCGGCCGC ACGCAGCCTG 151 GCGGCCGCCT GAGCTACTTC ACCCTCCGCC GGTAAGTGAC TGCAAACATC 201 ATTCATTCAA TCAGCCTCAC TGGGAGCCCC TTCTCTCCGG CTGGTAGTCC 251 TGGGCGGCTT GTCCCTGATC CCGAGCGGGG CTTGGCACAG CATCAGCCCT 301 GGAGGGCAGG CAGCAGGTGC CTTTGCCTGG TGGGTCCACT GGGGAGCGTG 351 GCTGGGGTTC GCGGCGGGTG CTGCCACCCA ACCTGCGGGC GGCGGGCTCG 401 CCCAGTAGGC GCCTCTCTGG TGAGAGGAGG CGGCTCCAGC CCGCATCCTG 451 GGGTAGTTGC TACTATTGGC CCCCAGCGCC CGCTCTGCGC GCGCGCCGTT 501 TCTGGCGGAT CCCCAGTGCG CGGCGCGCTG TTTACACCGG CGTGGTACTA 551 GTCACGGAGC CGCACCCCTC GGAAAGCGCG GAGTCGATGA CAGCCACTTC 601 ACAGGCTCAC GCGCTCCTAG TGTGGGCTTG AAGGGGACGG GGACCGATTA 651 CCAAAGGAGA GCGCTGAGTA CGGAAGACAC AGGGCAGCCT TTGTCTTGGG 701 TTTAGCGCTG ATGCGCTCAA CCCTGAGTCG GGTTCACTGC AACTGTTGTG 751 TCCGATTTCG GTTCCCTGCA ACCGCCCTCC TGGGCGAGAG ATGTCATTGT 801 GTTCCTGCGG CCAGCGGGAC TGAGAGCTGG GACTTAAGAC GCCAGGAGGG 851 TCCTGCGCTC ACGGGAAATG TACCCCAAAA GAACTCTGAG AGAATATACT 901 CAACTGTCCT GCTGTGATTA AACAAGACTG CTGTATTTTA ATTTCAGAAA 951 TTGAAAAGGG ATAGGAGGAA GGGGAAAATG CTGGGCTGGT GTGAAGCGAT 1001 AGCCCGTAAC CCTCACAGAA TTCCAAACAA CACGCGAACA CCCGAGATCT 1051 CAGGGGATTT GGCTGACGCC TCACAAACCT CCACATTGAA TGAAAAATCC 1101 CCAGGGCGAT CTGCAAGTCG ATCAAGTAAC ATTTCAAAAG CAAGCAGCCC 1151 AACAACAGGG ACAGCTCCCA GGAGCCAGTC AAGGTTGTCT GTCTGTCCAT 1201 CCACTCAGGA CATCTGCAGA ATCTGTCACT GCGAAGGGGA TGAAGAGAGC 1251 CCCCTCATCA CACCCTGTCG CTGCACTGGG ACACTGCGCT TTGTCCACCA 1301 GTCCTGCCTC CACCAGTGGA TAAAGAGCTC AGATACACGC TGCTGTGAGC 1351 TCTGCAAGTA TGACTTCATA ATGGAGACCA AGCTCAAACC CCTCCGGAAG 1401 TGGGAGAAAC TACAGATGAC CACAAGTGAA AGGAGGAAAA TATTCTGCTC 1451 TGTCACATTC CACGTAATCG CGATCACCTG TGTGGTTTGG TCTTTGTATG 1501 TATTGATAGA CCGGACAGCG GAGGAAATCA AGCAAGGCAA TGACAATGGT 1551 GTCCTTGAAT GGCCATTTTG GACAAAACTG GTTGTGGTAG CCATTGGCTT 1601 CACAGGAGGT CTTGTCTTCA TGTACGTACA GTGTAAAGTC TATGTTCAGT 1651 TGTGGCGCAG GCTGAAGGCC TACAACCGTG TGATCTTTGT ACAAAATTGC 1701 CCAGACACTG CCAAAAAACT GGAGAAGAAC TTCTCATGTA ATGTAAACAC 1751 AGACATCAAA GATGCTGTGG TAGTGCCTGT ACCACAAACA GGTGCAAATT 1801 CACTGCCATC TGCAGAGGGT GGCCCCCCTG AAGTTGTATC AGTCTGATGG 1851 AACCTGTTGG GAGTTTCTTC ACCGAAGAAT ATCTTTCTAG CCCTCAGCCA 1901 CTACAAATGA CAGAAGTGAC CTTGAATTAT TTACTCCCTT CAGCTCCTCC 1951 TTTCTCCTAC TGACACATTT TTCCTGACTT TGTTCAAAGA GGAAAGGAGA 2001 AAAACAAACA AACAGACCAA ATGCCCAGGA GCCCATGAAG TAATAGCGTA 2051 AAGTAAAGTA TGATATGGAA ATGTGAAGTT TGCAAGAGAA TGATTTCCAA 2101 GACAATTAAG AACTACTGGG GCAATGAATG CTTTTAGGCA GTAATCAAAG 2151 ATTAAATGGA CCCATGATAC TCTTCTTCAC AGTAACAGGG GAAAAGTTCA 2201 AGAATACAGA CTTGAATTGC GATGTGTATT ACTTCTAGGG CCTTGTAATG 2251 TTAACTGTCT CATCTGGAAA TAATAACTAA CATATTTGGT TTTAAGCCTG 2301 AAATTGTCTG CATTATCCCT AAGTCACATT GGAAGTGAAC TTGGAGGATG 2351 CATATTTTGA TATGCTTTGA CAGCTAACAG ATTTGTATGG TTTAGTGGAG 2401 TCTGGTTATT TTGACAGATG CATGTTTTTT TTAAATAGAT GCAATATACA 2451 TTTGAAGACA TTGATATTTG GAATTAATTA TGTTTGTTTA AGTCACGCAA 2501 AAGATTTTCA GAAAATGTTC GGATATAATT AGCTCTGTTA AATACCCACA 2551 GAACTGTTAT CAGGTCTTAT ATTTATTTTC ATCTGGTTCC TCTAATACAG 2601 TGCTGTCCAA TAGAAACACA ACAGCCACAA ATGCAGGCCA CAGATGCAAA 2651 TATTTAACTT CCCAGTAGCC CTATTTTAAA AAGTAAAAAT AAATGTTTGT 2701 TTGTTAAAAA AAAAA BLAST Results Entry G37457 from database EMBLNEW: SHGC-57357 Human Homo sapiens STS genomic. Length = 458 Plus Strand HSPs: Score = 2116 (317.5 bits), Expect = 4.3e-91, P = 4.3e-91 Identities = 444/456 (97%) Medline entries No Medline entry Peptide information for frame 3 1 MLGWCEAIAR NPHRIPNNTR TPEISGDLAD ASQTSTLNEK SPGRSAΞRSS 51 NISKASSPTT GTAPRSQSRL SVCPSTQDIC RICHCEGDEE SPLITPCRCT 101 GTLRFVHQSC LHQWIKSSDT RCCELCKYDF IMETKLKPLR KWEKLQMTTS 151 ERRKIFCSVT FHVIAITCVV WSLYVLIDRT AEEIKQGNDN GVLEWPFWTK 201 LVVVAIGFTG GLVFMYVQCK VYVQLWRRLK AYNRVIFVQN CPDTAKKLEK 251 NFSCNVNTDI KDAVVVPVPQ TGANSLPSAE GGPPEVVSV ORF from 978 bp to 1844 bp; peptide length: 289 Category: similarity to unknown protein BLASTP hits Entry AB011169_1 from database TREMBL: gene: "KIAA0597"; product: "KIAA0597 protein"; Homo sapiens mRNA for KIAA0597 protein, partial eds. Score = 188, P = 6.0e-12, identities = 30/54, positives = 38/54 Entry SPBC14F5_7 from database TREMBL: gene: "SPBC14F5.07"; product: "hypothetical protein"; S. pombe chromosome II cosmid cl4F5. Score = 185, P = 1.9e-ll, identities = 29/53, positives = 38/53 Entry CEY57A10B_1 from database TREMBL: gene: "Y57A10B.1"; Caenorhabditis elegans cosmid Y57A10B Score = 171, P = 2.6e-10, identities = 40/107, positives = 58/107 Alert BLASTP hits for DKFZphfbr2_82ml6, frame 3 TREMBL :ATF28A23_14 gene: "F28A23.140"; product: "putative protein"; Arabidopsis thaliana DNA chromosome 4, BAC clone F28A23 (ESSAII project), N = 1, Score = 198, P = 3.4e-13 >TREMBL:ATF28A23_14 gene: "F28A23.140"; product: "putative protein"; Arabidopsis thaliana DNA chromosome 4, BAC clone F28A23 (ESSAII project) Length = 1,051 HSPs: Score = 198 (29.7 bits), Expect = 3.4e-13, P = 3.4e-13 Identities = 38/103 (36%), Positives = 61/103 (59%) Query: 28 LADASQTSTLNEKSPGRSASRS-SNISKASSPTTGTAPRSQSRLSVCPSTQDICRICHCE 86 +++ S +S+ + SP +++ SN+ A S TG+ +D+CRIC Sbjct: 20 VSEPSVSSSΞSSSSPNQASPNPFSNMDPAVSTATGSRYVDDDE DEEDVCRICRNP 74 Query: 87 GDEESPLITPCRCTGTLRFVHQΞCLHQWIKSSDTRCCELCKYDF 130 GD ++PL PC C+G+++FVHQ CL QW+ S+ R CE+CK+ F Sbjct: 75 GDADNPLRYPCACSGSIKFVHQDCLLQWLNHSNARQCEVCKHPF 118 Pedant information for DKFZphfbr2_82ml 6 , frame 3 Report for DKFZphfbr2_82ml 6 . 3 [ LENGTH ] 289 [MW] 32308 . 36 [pi ] 8 . 76 [HOMOL] PIR:T00268 hypothetical protein KIAA0597 - human (fragment) 9e-14 [FUNCAT] 04.99 other transcription activities [S. cerevisiae, YIL030c] 4e-09 [PIRKW] transmembrane protein 9e-08 [PROSITE] MYRISTYL 1 [PROSITE] CK2_PHOSPHO_SITE 4 [PROSITE] TYR_PHOSPHO_SITE 1 [PROSITE] PKC_PHOΞPHO_SITE 3 [PROSITE] ASN_GLYCOSYLATION 3 [KW] Alpha_Beta [KW] LOW COMPLEXITY 6.57 % SEQ MLGWCEAIARNPHRIPNNTRTPEISGDLADASQTSTLNEKSPGRSASRSSNISKASSPTT SEG xxxxxxxxxxxxxxxxxxx .. PRD ccchhhhhhccccccccccccccccchhhhhhhhhccccccccccccccccccccccccc SEQ GTAPRSQSRLSVCPSTQDICRICHCEGDEESPLITPCRCTGTLRFVHQSCLHQWIKSSDT SEG PRD ccccccccccccccccceeeeeeecccccccccccccccccceeeeehhhhhhhhhcccc SEQ RCCELCKYDFIMETKLKPLRKWEKLQMTTSERRKIFCSVTFHVIAITCVVWSLYVLIDRT SEG PRD ceeeeeeehhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccc SEQ AEEIKQGNDNGVLEWPFWTKLVVVAIGFTGGLVFMYVQCKVYVQLWRRLKAYNRVIFVQN SEG PRD ccccccccccceeehhhhheeeeeeecccccceeeeehhhhhhhhhhhhhhhheeeeeee SEQ CPDTAKKLEKNFSCNVNTDIKDAVVVPVPQTGANSLPSAEGGPPEVVSV SEG PRD ccchhhhhhccccccccccceeeeeeecccccccccccccccccccccc Prosite for DKFZphfbr2_82ml6.3 PS00001 17->21 ASN_GLYCOSYLATION PDOC00001 PS00001 51->55 ASN_GLYCOSYLATION PDOC00001 PS00001 251->255 ASN_GLYCOSYLATION PDOC00001 PS00005 102-M 05 PKC_PHOSPHO_SITE PDOC00005 PS00005 150->153 PKC_PHOS PHO_S ITE PDOC00005 PS00005 244 ->247 PKC_PHOS PHO_S ITE PDOC00005 PS00006 36->40 CK2_PHOS PHO_S ITE PDOC00006 PS00006 75->79 CK2_PHOSPHO_SITE PDOC00006 PS00006 148 ->152 CK2_PHOSPHO_SITE PDOC00006 PS00006 180-M84 CK2_PHOSPHO_SITE PDOC00006 PS00007 121 -> 129 TYR_PHOSPHO_S ITE PDOC00007 PS00008 187 ->193 MYRISTYL PDOC00008 (No Pfam data available for DKFZphfbr2_82ml6.3) DKFZphfbr2_82m6 group: signal transduction DKFZphfbr2_82m6.3 encodes a novel 654 amino acid protein with similarity to murine sphingosine kinase . Sphingosine kinase is a new type of lipid kinase, which is regulated by growth factors. The enzyme phosphorylates sphingosine, which subsequently exerts intracellular and extracellular actions. Intracellulary, sphingosine 1-phosphate (SPP) promotes proliferation and inhibits apoptosis. In yeast, survival of cells exposed to heat shock indicates is dependend on SPP. Extracellulary, SPP inhibits cell motility and influences cell morphology, effects that appear to be mediated by the G protein-coupled receptor EDG1. The new protein can find application in modulating/blocking the shingosine kinase intracellular signal transmission pathway. strong similarity to mouse "sphingosine kinase" complete cDNA, complete eds, EST hits, YLR260w/YOR171c Lcb5p/Lcb4p = long chain base kinases, involved in biosynthesis of sphingol pids Sequenced by DKFZ Locus : unknown Insert length: 2875 bp Poly A stretch at pos. 2865, polyadenylation signal at pos. 2838 1 AGTGTTGGAG GTGAGGAGGC GGGGCTGGCA GGGCTAGTCG GGGCATCTGG 51 AAATTTCCGA CCCCACGCTT CGGGCGTTTC CTTATCAGGT TCACCGCTCC 101 CTGATCTCGC GCTGCACTTC GTAGGCGCAG CCGCTGCTTG GGAAGTCCTA 151 CTTAAGAGCT GAAGGTCAGG CCAGGACAGT GAGACCTGAC TCCTTGCTCC 201 TACCAGCCTA CTATGGCTTA AGACCCAGGG CCAGGGTCCC GTTGATGTAA 251 CAGAGCAGAG GACCAGCAGA TGAATGGACA CCTTGAAGCA GAGGAGCAGC 301 AGGACCAGAG GCCAGACCAG GAGCTGACCG GGAGCTGGGG CCACGGGCCT 351 AGGAGCACCC TGGTCAGGGC TAAGGCCATG GCCCCGCCCC CACCGCCACT 401 GGCTGCCAGC ACCTCGCTCC TCCATGGCGA GTTTGGCTCC TACCCAGCCC 451 GAGGCCCACG CTTTGCCCTC ACCCTTACAT CGCAGGCCCT GCACATACAG 501 CGGCTGCGCC CCAAACCTGA AGCCAGGCCC CGGGGTGGCC TGGTCCCGTT 551 GGCCGAGGTC TCAGGCTGCT GCACCCTGCG AAGCCGCAGC CCCTCAGACT 601 CAGCGGCCTA CTTCTGCATC TACACCTACC CTCGGGGCCG GCGCGGGGCC 651 CGGCGCAGAG CCACTCGCAC CTTCCGGGCA GATGGGGCCG CCACCTACGA 701 AGAGAACCGT GCCGAGGCCC AGCGCTGGGC CACTGCCCTC ACCTGTCTGC 751 TCCGAGGACT GCCACTGCCC GGGGATGGGG AGATCACCCC TGACCTGCTA 801 CCTCGGCCGC CCCGGTTGCT TCTATTGGTC AATCCCTTTG GGGGTCGGGG 851 CCTGGCCTGG CAGTGGTGTA AGAACCACGT GCTTCCCATG ATCTCTGAAG 901 CTGGGCTGTC CTTCAACCTC ATCCAGACAG AACGACAGAA CCACGCCCGG 951 GAGCTGGTCC AGGGGCTGAG CCTGAGTGAG TGGGATGGCA TCGTCACGGT 1001 CTCGGGAGAC GGGCTGCTCC ATGAGGTGCT GAACGGGCTC CTAGATCGCC 1051 CTGACTGGGA GGAAGCTGTG AAGATGCCTG TGGGCATCCT CCCCTGCGGC 1101 TCGGGCAACG CGCTGGCCGG AGCAGTGAAC CAGCACGGGG GATTTGAGCC 1151 AGCCCTGGGC CTCGACCTGT TGCTCAACTG CTCACTGTTG CTGTGCCGGG 1201 GTGGTGGCCA CCCACTGGAC CTGCTCTCCG TGACGCTGGC CTCGGGCTCC 1251 CGCTGTTTCT CCTTCCTGTC TGTGGCCTGG GGCTTCGTGT CAGATGTGGA 1301 TATCCAGAGC GAGCGCTTCA GGGCCTTGGG CAGTGCCCGC TTCACACTGG 1351 GCACGGTGCT GGGCCTCGCC ACACTGCACA CCTACCGCGG ACGCCTCTCC 1401 TACCTCCCCG CCACTGTGGA ACCTGCCTCG CCCACCCCTG CCCATAGCCT 1451 GCCTCGTGCC AAGTCGGAGC TGACCCTAAC CCCAGACCCA GCCCCGCCCA 1501 TGGCCCACTC ACCCCTGCAT CGTTCTGTGT CTGACCTGCC TCTTCCCCTG 1551 CCCCAGCCTG CCCTGGCCTC TCCTGGCTCG CCAGAACCCC TGCCCATCCT 1601 GTCCCTCAAC GGTGGGGGCC CAGAGCTGGC TGGGGACTGG GGTGGGGCTG 1651 GGGATGCTCC GCTGTCCCCG GACCCACTGC TGTCTTCACC TCCTGGCTCT 1701 CCCAAGGCAG CTCTACACTC ACCCGTCTCC GAAGGGGCCC CCGTAATTCC 1751 CCCATCCTCT GGGCTCCCAC TTCCCACCCC TGATGCCCGG GTAGGGGCCT 1801 CCACCTGCGG CCCGCCCGAC CACCTGCTGC CTCCGCTAGG CACCCCGCTG 1851 CCCCCAGACT GGGTGACGCT GGAGGGGGAC TTTGTGCTCA TGTTGGCCAT 1901 CTCGCCCAGC CACCTAGGCG CTGACCTGGT GGCAGCTCCG CATGCGCGCT 1951 TCGACGACGG CCTGGTGCAC CTGTGCTGGG TGCGTAGCGG CATCTCGCGG 2001 GCTGCGCTGC TGCGCCTTTT CTTGGCCATG GAGCGTGGTA GCCACTTCAG 2051 CCTGGGCTGT CCGCAGCTGG GCTACGCCGC GGCCCGTGCC TTCCGCCTAG 2101 AGCCGCTCAC ACCACGCGGC GTGCTCACAG TGGACGGGGA GCAGGTGGAG 2151 TATGGGCCGC TACAGGCACA GATGCACCCT GGCATCGGTA CACTGCTCAC 2201 TGGGCCTCCT GGCTGCCCGG GGCGGGAGCC CTGAAACTAA ACAAGCTTGG 2251 TACCCGCCGG GGGCGGGGCC TACATTCCAA TGGGGCGGAG CCTGAGCTAG 2301 GGGGTGTGGC CTGGCTGCTA GAGTTGTGGT GGCAGGGGCC CTGGCCCCGT 2351 CTCAGGATTG CGCTCGCTTT CATGGGACCA GACGTGATGC TGGAAGGTGG 2401 GCGTCGTCAC GGTTAAAGAG AAATGGGCTC GTCCCGAGGG TAGTGCCTGA 2451 TCAATGAGGG CGGGGCCTGG CGTCTGATCT GGGGCCGCCC TTACGGGGCA 2501 GGGCTCAGTC CTGACGCTTG CCACCTGCTC CTACCCGGCC AGGATGGCTG 2551 AGGGCGGAGT CTATTTTACG CGTCGCCCAA TGACAGGACC TGGAATGTAC 2601 TGGCTGGGGT AGGCCTCAGT GAGTCGGCCG GTCAGGGCCC GCAGCCTCGC 2651 CCCATCCACT CCGGTGCCTC CATTTAGCTG GCCAATCAGC CCAGGAGGGG 2701 CAGGTTCCCC GGGGCCGGCG CTAGGATTTG CACTAATGTT CCTCTCCCCG 2751 CGGGTGGGGG CGGGGAAATT CATATCCCCT GTTCGTCTCA TGCGCGTCCT 2801 CCGTCCCCAA TCTAAAAAGC AATTGAAAAG GTCTATGCAA TAAAGGCAGT 2851 CGCTTCATTC CTCTCAAAAA AAAAA BLAST Results No BLAST result Medline entries 99045661: Tumor necrosis factor-alpha induces adhesion molecule expression through the sphingosine kinase pathway. 98395082: Molecular cloning and functional characterization of murine sphingosine kinase. 98241633: Purification and characterization of rat kidney sphingosine kinase. 99178622: Sphingosine 1-phosphate: a prototype of a new class of second messengers . Peptide information for frame 3 1 MNGHLEAEEQ QDQRPDQELT GSWGHGPRST LVRAKAMAPP PPPLAASTSL 51 LHGEFGSYPA RGPRFALTLT SQALHIQRLR PKPEARPRGG LVPLAEVSGC 101 CTLRSRSPSD SAAYFCIYTY PRGRRGARRR ATRTFRADGA ATYEENRAEA 151 QRWATALTCL LRGLPLPGDG EITPDLLPRP PRLLLLVNPF GGRGLAWQWC 201 KNHVLPMISE AGLSFNLIQT ERQNHARELV QGLSLSEWDG IVTVSGDGLL 251 HEVLNGLLDR PDWEEAVKMP VGILPCGSGN ALAGAVNQHG GFEPALGLDL 301 LLNCSLLLCR GGGHPLDLLS VTLASGSRCF SFLSVAWGFV SDVDIQSERF 351 RALGSARFTL GTVLGLATLH TYRGRLSYLP ATVEPASPTP AHSLPRAKSE 401 LTLTPDPAPP MAHSPLHRSV SDLPLPLPQP ALASPGSPEP LPILSLNGGG 451 PELAGDWGGA GDAPLSPDPL LSSPPGSPKA ALHSPVSEGA PVIPPSSGLP 501 LPTPDARVGA STCGPPDHLL PPLGTPLPPD WVTLEGDFVL MLAISPSHLG 551 ADLVAAPHAR FDDGLVHLCW VRSGISRAAL LRLFLAMERG SHFSLGCPQL 601 GYAAARAFRL EPLTPRGVLT VDGEQVEYGP LQAQMHPGIG TLLTGPPGCP 651 GREP ORF from 270 bp to 2231 bp; peptide length: 654 Category: similarity to known protein BLASTP hits Entry SPAC4A8_7 from database TREMBL: gene: "SPAC4A8.07c"; product: "hypothetical protein"; S. pombe chromosome I cosmid c4A8. Score = 301, P = 7.9e-32, identities = 68/190, positives = 109/190 Entry CEC34C6_3 from database TREMBLNEW: product: "C34C6.5"; Caenorhabditis elegans cosmid C34C6 >TREMBL:CEC34C6_3 product: "C34C6.5"; Caenorhabditis elegans cosmid C34C6 Score = 273, P = 9.0e-29, identities = 78/265, positives = 142/265 Entry S67059 from database PIR: hypothetical protein YOR171c - yeast (Saccharomyces cerevisiae) >TREMBL:SC55021_9 gene: "03615"; product: "03615p"; Saccharomyces cerevisiae cosmid pUOA1258 from chromosome 15R. >TREMBL: SCYOR170W_2 S. cerevisiae chromosome XV reading frame ORF YOR170w Score = 253, P = 2.0e-25, identities = 70/234, positives = 116/234 Entry S51398 from database PIR: hypothetical protein YLR260w - yeast (Saccharomyces cerevisiae) >TREMBL:SCL8479_4 gene: "YLR260W"; product: "Ylr260wp"; Saccharomyces cerevisiae chromosome XII cosmid 8479. Score = 251, P = 1.0e-24, identities = 62/198, positives = 103/198 Alert BLASTP hits for DKFZphfbr2_82m6, frame 3 TREMBL:AF068749_1 gene: "SPHKlb"; product: "sphingosine kinase"; Mus musculus sphingosine kinase (SPHKlb) mRNA, complete eds., N = 2, Score = 615, P = 1.2e-92 TREMBL:AF068748_1 gene: "SPHKla"; product: "sphingosine kinase"; Mus musculus sphingosine kinase (SPHKla) mRNA, partial eds., N = 2, Score = 616, P = 2e-92 TREMBL:ATF18E5_16 gene: "F18E5.160"; product: "putative protein"; Arabidopsis thaliana DNA chromosome 4, BAC clone F18E5 (ESSAII project), N = 2, Score = 370, P = 6.8e-33 >TREMBL:AF068748_1 gene: "SPHKla"; product: "sphingosine kinase"; Mus musculus sphingosine kinase (SPHKla) mRNA, partial eds. Length = 504 HSPs: Score = 616 (92.4 bits), Expect = 2.0e-92, Sum P(2) = 2.0e-92 Identities = 128/260 (49%), Positives = 173/260 (66%) Query: 154 ATALTCLLRGLPLPGDGEITPDLLPRPPRLLLLVNPFGGRGLAWQWCKNHVLPMISEAGL 213 A C L + E LLPRP R+L+L+NP GG+G A Q ++ V P + EA + Sbjct: 110 APVAPCQREPRDLAMEPECPRGLLPRPCRVLVLLNPQGGKGKALQLFQSRVQPFLEEAEI 169 Query: 214 SFNLIQTERQNHARELVQGLSLSEWDGIVTVSGDGLLHEVLNGLLDRPDWEEAVKMPVGI 273 +F LI TER+NHARELV L WD + +SGDGL+HEV+NGL++RPDWE A++ P+ Sbjct: 170 TFKLILTERKNHARELVCAEELGHWDALAVMSGDGLMHEVVNGLMERPDWETAIQKPLCS 229 Query: 274 LPCGSGNALAGAVNQHGGFEPALGLDLLLNCSLLLCRGGGHPLDLLSVTLASGSRCFSFL 333 LP GSGNALA +VN + G+E DLL+NC+LLLCR P++LLS+ ASG R +Ξ L Sbjct: 230 LPGGSGNALAASVNHYAGYEQVTNEDLLINCTLLLCRRRLSPMNLLSLHTASGLRLYSVL 289 Query: 334 SVAWGFVSDVDIQSERFRALGSARFTLGTVLGLATLHTYRGRLSYLPA-TVEPASPTPAH 392 S++WGFV+DVD++SE++R LG RFT+GT LA+L Y+G+L+YLP TV AS PA Sbjct: 290 SLSWGFVADVDLESEKYRRLGEIRFTVGTFFRLASLRIYQGQLAYLPVGTV—ASKRPAS 347 Query: 393 SL-PRAKSELTLTPDPAPPMAH 413 +L + + L P P +H Sbjct: 348 TLVQKGPVDTHLVPLEEPVPSH 369 Score = 324 (48.6 bits), Expect = 2.0e-92, Sum P(2) = 2.0e-92 Identities = 72/160 (45%), Positives = 100/160 (62%) Query: 499 LPLPTPDARVGASTC GPPDHLLPPLGTPLPPDWVTL-EGDFVLMLAISPΞHLGADLV 554 LP+ T ++ AST GP D L PL P+P W + E DF+L+L + +HL ++L Sbjct: 335 LPVGTVASKRPASTLVQKGPVDTHLVPLEEPVPSHWTVVPEQDFLLVLVLLHTHLSSELF 394 Query: 555 AAPHARFDDGLVHLCWVRSGISRAALLRLFLAMERGSHFSLGCPQLGYAAARAFRLEPLT 614 AAP R + G++HL +VR+G+SRAALLRLFLAM++G H L CP L + AFRLEP + Sbjct: 395 AAPMGRCEAGVMHLFYVRAGVSRAALLRLFLAMQKGKHMELDCPYLVHVPVVAFRLEPRS 454 Query: 615 PRGVLTVDGEQVEYGPLQAQMHPGIGTLLTGPPGCP-GRE 653 RGV +VDGE + +Q Q+HP ++ G P GR+ Sbjct: 455 QRGVFSVDGELMVCEAVQGQVHPNYLWMVCGSRDAPSGRD 494 Score = 37 (5.6 bits), Expect = 3.6e-62, Sum P(2) = 3.6e-62 Identities = 8/20 (40%), Positives = 9/20 (45%) Query: 459 GAGDAPLSPDPLLSSPPGSP 478 G+ DAP D PP P Sbjct: 485 GSRDAPSGRDSRRGPPPEEP 504 Pedant information for DKFZphfbr2_82m6, frame 3 Report for DKFZphfbr2 82m6.3 [LENGTH] 654 [MW] , 69207.45 [pi] 6.47 [HOMOL] TREMBL:AF068749_1 gene: "SPHKlb"; product: "sphingosine kinase"; Mus musculus sphingosine kinase (SPHKlb) mRNA, complete eds. 2e-50 [FUNCAT] 01.06.01 lipid, fatty-acid and sterol biosynthesis [S. cerevisiae, YLR260w] 4e-20 [PROSITE] AMIDATION 1 [PROSITE] CAMP_PHOSPHO_SITE 1 [PROSITE] MYRISTYL 12 [PROSITE] CK2_PHOSPHO_SITE 6 [PROSITE] TYR_PHOSPHO_SITE 1 [PROSITE] GLYCOSAMINOGLYCAN 1 [PROSITE] PKC_PHOSPHO_SITE 8 [PROSITE] ASN_GLYCOSYLATION 1 [KW] Alpha_Beta [KW] LOW_COMPLEXITY 20.18 % SEQ MNGHLEAEEQQDQRPDQELTGSWGHGPRSTLVRAKAMAPPPPPLAASTSLLHGEFGSYPA SEG xxxxxxxxxxxxx PRD ccchhhhhhhhcccccceeecccccccceeehhhhhccccccceeeceeeeccccccccc SEQ RGPRFALTLTSQALHIQRLRPKPEARPRGGLVPLAEVSGCCTLRΞRSPSDSAAYFCIYTY SEG PRD cccceeehhhhhhhhhhhhhccccccccccceeeeeeeceeeeeecccccceeeeeeeec SEQ PRGRRGARRRATRTFRADGAATYEENRAEAQRWATALTCLLRGLPLPGDGEITPDLLPRP SEG .xxxxxxxxxxxxxxxxxxxxx xxxxx PRD ccccchhhhhhhhhhhcccchhhhhhhhhhhhhhhhhhhhhccccccccccccccccccc SEQ PRLLLLVNPFGGRGLAWQWCKNHVLPMISEAGLSFNLIQTERQNHARELVQGLSLSEWDG SEG xxxxxx PRD ceeeeeeecccccchhhhhhhhhhhhhhhhhccchhhhhhhhhhhhhhhhhhhhccccce SEQ IVTVSGDGLLHEVLNGLLDRPDWEEAVKMPVGILPCGSGNALAGAVNQHGGFEPALGLDL SEG xxxxx PRD eeeecccccceeeccccccccchhhhhccceeeccccccccccccccccccccchhhhhh SEQ LLNCSLLLCRGGGHPLDLLSVTLASGSRCFSFLSVAWGFVSDVDIQSERFRALGSARFTL SEG xxxxxxxxxxxxx PRD hhhhhhccccccccccceeeeeeccccceeeeeeeeccccceeeehhhhhhhhhhhhhhc SEQ GTVLGLATLHTYRGRLSYLPATVEPASPTPAHSLPRAKSELTLTPDPAPPMAHSPLHRSV SEG PRD hhhhhhhhhhhhcccccccccccccccccccccccccccccccccccccccccccccccc SEQ SDLPLPLPQPALASPGSPEPLPILSLNGGGPELAGDWGGAGDAPLSPDPLLSSPPGSPKA SEG ..xxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxx PRD ccccccccccccccccccccceeeeeccccccccccccccccccccccccccccccccce SEQ ALHSPVΞEGAPVIPPSSGLPLPTPDARVGASTCGPPDHLLPPLGTPLPPDWVTLEGDFVL SEG xx xxxxxxxxxxxxxxx PRD eeccccccccccccccccccccccccccccccccccccccccccccccccccccccccee SEQ MLAISPSHLGADLVAAPHARFDDGLVHLCWVRSGISRAALLRLFLAMERGSHFSLGCPQL SEG PRD eeeeecccccccccccccccccccceeeeeeeccchhhhhhhhhhhhhcccceeecccch SEQ GYAAARAFRLEPLTPRGVLTVDGEQVEYGPLQAQMHPGIGTLLTGPPGCPGREP SEG \ xxxxxxxxxxxxxxx ... PRD hhhhhhhhhhccccccceeeeccceeecccccccccccccceeecccccccccc Prosite for DKFZphfbr2_82m6.3 PS00001 303- ->307 ASN GLYCOSYLATION PDOC00001 PS00002 245- ->249 GLYCOSAMINOGLYCAN PDOC00002 PS00004 129- ->133 CAMP PHOSPHO SITE PDOC00004 PS00005 102- ->105 PKC PHOSPHO SITE PDOC00005 PS00005 134- ->137 PKC PHOSPHO SITE PDOC00005 PS00005 220- ->223 PKC PHOSPHO SITE PDOC00005 PS00005 347- ->350 PKC PHOSPHO SITE PDOC00005 PS00005 355- ->358 PKC PHOSPHO SITE PDOC00005 PS00005 371- ->374 PKC PHOSPHO SITE PDOC00005 PS00005 477- ->480 PKC PHOSPHO SITE PDOC00005 PS00005 614- ->617 PKC PHOSPHO SITE PDOC00005 PS00006 107- ->111 CK2 PHOSPHO SITE PDOC00006 o o CO H U α. co co r— 0000000000 oo o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o υ o o o o o υ o υ o o υ o o o o o o o o o o o o o o o o o o o o o Q Q Q a ω u a o a a ^^ ^^^ r ∞co cocoωcoω∞co co co oo o OOOOOOOOOOOOOOOOOOO OOOOOOOOOOOOOOOOOOO o OOOOOOOOOOOOOOOOOOO OOOOOOOOOOOOOOOOOOO o DKFZphfkd2_lj9 group: kidney derived DKFZphfkd2_lj9.3 encodes a novel 105 ammo acid protein with high similarity to Xenopus laevis XLCL2 protein. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of kidney-specific genes . strong similarity to XLCL2 protein, African clawed frog complete cDNA, complete eds, EST hits Sequenced by LMU Locus: unknown Insert length: 2955 bp Poly A stretch at pos. 2935, polyadenylation signal at pos. 2915 1 GGGGGGGGCT GAGTGCTCAG TGGAGAGCGG GGAGTTGTGT CCACCTTGCC 51 GACGTCGCTA GCCGTGGGGC TGTCCTGGGA AGGCGGACGG CGAGCGCCCG 101 GTGTCCGCAC TCGGCCGCCT GCCGTGCCCG TCTGCGCCCG TGTCATCCTC 151 ACTCGGGACG CAGGGACCGT TTTTAAATCA CAGGGGCGTG TGTCAGCCTG 201 CCCTAGGACT TCATGTCTAT ATATTTCCCC ATTCACTGCC CCGACTATCT 251 GAGATCGGCC AAGATGACTG AGGTGATGAT GAACACCCAG CCCATGGAGG 301 AGATCGGCCT CAGCCCCCGC AAGGATGGCC TTTCCTACCA GATCTTCCCA 351 GACCCGTCAG ATTTTGACCG CCGCTGCAAA CTGAAGGACC GTCTGCCCTC 401 CATAGTGGTG GAACCCACAG AAGGGGAGGT GGAGAGCGGG GAGCTCCGGT 451 GGCCCCCTGA GGAGTTCCTG GTCCAGGAGG ATGAGCAAGA TAACTGCGAA 501 GAGACAGCGA AAGAAAATAA AGAGCAGTAG AGTCCCTGTG GACTCCCATG 551 GGTCATACCA GCCAGCATCT GTTCCTGAAC TGTGTTTTTC CCATCATGAC 601 GGAAGAAGAG AGTGAGCCGC AATTGTTCTG AAAATGTCAA ACGAGGCTTC 651 TGTTTTGCAC CTGCAGATCA CCGAGTTGGT TTTCTTTTCT TTTCTTGCCT 701 TTTTTTTTTT TTTGAAATTT GCCGAGCAGT GGAGCCCTCT GACAATTTGC 751 AAGGCCCTCT GAGAAAGGAA GCTGCTTAGA GCCAGGGGGT TAGTGGGTGA 801 GGGGAGCGAG TGCTGTTTTT GAGATCATTA TCTGAACTCA GGCAGCCTAG 851 TAGAGGCAGT GGTGGGATTC CAATGGGTCT TGGTGGGTGG GAGGTGGGGC 901 ATGTGCAAAG CAAGCAAGGA ACATTTGGGG TAAGAAAACA AACATGAGGC 951 AAAAGAAAAA ATACATGTTT TTAAGAAAAC ATTGAGCAGA GAACTGCAGC 1001 CAGGATGCGC TCAGCAGACA TTCACTCTGG CCGCTGGGAC ATCAGAAAAC 1051 AAAGTCTTCA TCTCTCTCTC CAGTTTCACC CACCCCACCC TTTGCTTTCA 1101 TTTCAGGTGT GTTGGTCTAT ATGACAGGGA GGAGAGTAAA GGAGAGCAGG 1151 AGCAATTGGC TGCCTGCAAA GCCAGCTGGA GGTGAAGTGC AGGAAAGGAA 1201 AGGTCACCCC ATTCTACTCC ATGGCCTCTC TGCTCCCAGC TGTGGTAGGC 1251 TCACATAGCC AGTGTGATCG GTTTTTAAGA GGCAGTGCTT TTCAGCTTTT 1301 CTCCCTGATA TATCCATTTT GCTTCCCAGC ACTTTTTAGG AGTAGTGAGA 1351 GCACTTCCTG CCCTTGTTGG AAGCCCCAGG GTGGACACTC AGCACGAAGG 1401 TCTCTCCCTT AACTGCTGCC CTTCCAAGAC TTGCTCCCGA GATGGAGTGG 1451 GCGTGGTCTT CCAGGCTGGC CCTTCCTTCT CCTCACCGCC ACCTTCCCTG 1501 CCCCAGCCCC AGCAGCCATG GGTACATGGG TCCCCAGCTC ACCTATGGAT 1551 TCCCGCCAGT CTGCCCAGCT GCAGTACTCA CGCCCCATGG GGGATCTTGG 1601 TCTGTTTTTC TTGTGGGAGC CTAGTGGAGA GCAGACGTGG CTTTTTATGT 1651 GTCTTGTTGG GGAGGTGACT TGCATGGTGG GGACAAGGCT GTCGTGGCAA 1701 CCTTGGGATC GAGTTTGAGA CTAAAGGATG TCATGAGATC CCTGGCTTCT 1751 CCCCATGTTG TTCCCGGACA AGGGCAGAAG GGAGGCATGG CAAGGGACCT 1801 CTGCTGTCCT TACTCAACAG TGGTCCTCAT CCCTCCCCAC CTCCCACTGC 1851 TTCCTGCAAG GGCACCAGTT GTATGAGAAA GTTGGCCTTT GGACTTAGGA 1901 TTTCTTATTG TAGCTAAGAG CCATCTGAAG CAGCAGGTTG CAGGACAAAT 1951 GCTTCAGTCC GCCGAGAGCA GTACCGTGTG GCCAAGAGGT GGACTCAGAG 2001 CCTTCCTTGA GCTAAACTCG GCCAACCAAG GCACGCAGCA TGTCCCCTCA 2051 GGTCTCCAGT CAGTCCAGGT TGACCCTCAG TTCTGGACGT GTGTATATAG 2101 CTGTATTTAA TACCTCAAGG TCATTGTGGC TCTGGGGATG CCAGGGCAGG 2151 AGGACGAGGG TGCGCTGTGG ACACAGCAGT CCGCGGAATT CCGTTCTGGG 2201 AAGCCAATGG TCGCCGGCAC CCCTTGCTTC CTCCCTCTGT TGTCTGCCTG 2251 TGTGACACAC ATCAATGGCA ATAACTTCTT CCAACTCCTC GCAGAAGTGG 2301 GAGAGGCCGG CAGCCTGCAC CGAGAGGGGC TTTCCTCTCT CTTGCTCCCC 2351 GCTTCGTTCT GTTTTGGCTG CAGAGAGTGG TTCATCCATA CTCTCATTCC 2401 CTCGCCTCCC CTTGTGGACG GGGGTCTTGC CTTTTCAATT CCTGTGTTTT 2451 GGTGTCTTCC CTTATCTGCT ACCCTGAATC ACCTGTCCTG GTCTTGCTGT 2501 GTGATGGGAA CATGCTTGTA AACTGCGTAA CAAATCTACT TTGTGTATGT 2551 GTCTGTTTAT GGGGGTGGTT TATTATTTTT GCTGGTCCCT AGACCACTTT 2601 GTATGACCGT TTGCAGTCTG AGCAGGCCAG GGGCTGACAG CTAATGTCAG 2651 GACCCTCAGC GGTGGAGCCT GCTGGGGGGA CCCAGCTGCT CTTGGACAAG 2701 TGGCTGAGCT CCTATCTGGC CTCCTCTTTT CAAGTAATTT 2751 GTGTGTATTT CTAACTGATT GTATTGAAAA AATTCCTAGT ATTTCAGTAA 2801 AAATGCCTGT TGTGAGATGA ACCTCCTGTA ACTTCTATCT GTTCTTTTTT 2851 GAGGCTCAGG GAGAAACTAG CATTTTTTTT TTTCCAAACT ACTTTTTGTC 2901 ACTGTGACAG TTGTAAATAA AGTTTGAAAA TGCTCAAAAA AAAAAAAAAA 2951 AAAAC BLAST Results Entry HSG19750 from database EMBL: human STS A001X24. Score = 1050, P = 1.9e-39, identities = 212/213 Entry HSG20267 from database EMBL: human STS A005C12. Score = 610, P = 4. le-19, identities = 122/122 Medline entries No Medlme entry Peptide information for frame 3 ORF from 213 bp to 527 bp; peptide length: 105 Category: strong similarity to known protein Classification: unset 1 MSIYFPIHCP DYLRSAKMTE VMMNTQPMEE IGLSPRKDGL SYQIFPDPSD 51 FDRRCKLKDR LPSIVVEPTE GEVESGELRW PPEEFLVQED EQDNCEETAK 101 ENKEQ BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfkd2_lj 9, frame 3 PIR:S52241 XLCL2 protein - African clawed frog, N = 1, Score = 443, P = 8e-42 PIR:S52241 XLCL2 protein - African clawed frog, N = 1, Score = 443, P = 8.2e-42 >PIR:S52241 XLCL2 protein - African clawed frog Length = 102 HSPs: Score = 443 (66.5 bits), Expect = 8.0e-42, P = 8.0e-42 Identities = 80/104 (76%), Positives = 95/104 (91%) Query: 1 MSIYFPIHCPDYLRSAKMTEVMMNTQPMEEIGLSPRKDGLSYQIFPDPSDFDRRCKLKDR 60 MS+++PIHC DYLRSA+MTEV+MNTQ M+EIGLSPRKD SYQIFPDPSDF+R CKLKDR Sbjct: 1 MSVFYPIHCTDYLRSAEMTEVIMNTQSMDEIGLSPRKD—SYQIFPDPSDFERCCKLKDR 58 Query: 61 LPSIVVEPTEGEVESGELRWPPEEFLVQEDEQDNCEETAKENKE 104 LPSIVVEPTEG+VESGELRWPPEEF+V ED++ C++T KEN++ Sbjct: 59 LPSIVVEPTEGDVESGELRWPPEEFVVDEDKEGTCDQTKKENEQ 102 Pedant information for DKFZphfkd2_lj 9, frame 3 Report for DKFZphf d2_lj 9.3 [LENGTH] 105 [MW] 12269.78 [pi] 4.40 [HOMOL] PIR:S52241 XLCL2 protein - African clawed frog 5e-44 [KW] Alpha_Beta SEQ MSIYFPIHCPDYLRSAKMTEVMMNTQPMEEIGLSPRKDGLSYQIFPDPSDFDRRCKLKDR PRD cccccccccccchhhhhhhhhhhhcccccccccccccccceeeecccccccchhhhhhhc SEQ LPSIVVEPTEGEVESGELRWPPEEFLVQEDEQDNCEETAKENKEQ PRD ccceeeecccccccccccccccccceeeccccchhhhhhhhhccc (No Prosite data available for DKFZphfkd2_l 9.3) (No Pfam data available for DKFZphfkd2_lj9.3) DKFZphfkd2_24al5 group: transmembrane protein DKFZphfkd2_24al5 encodes a novel amino acid protein with similarity to C. elegans cosmid R07G3. The novel protein contains 1 transmembrane region. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of kidney-specific genes and as a new marker for kidney cells. similarity to C. elegans R07G3.8 membrane regions : 1 Summary DKFZphfkd2_24al5 encodes a novel 323 ammo acid protein, with similarity to C. elegans R07G3.8. similarity to C. elegans R07G3.8 complete cDNA, complete eds, EST hits Sequenced by GBF Locus: unknown Insert length: 1513 bp Poly A stretch at pos. 1494, no polyadenylation signal found 1 GGGGTACTCG GCGGCGGCGG AGCGGGCGGC AGAGCAGGGC GGCGGCGACT 51 CGCAGGGTAC CACCATCTTA AGGACAGAAA AGCTACAGGA CTCTAGGAGG 101 CCACCGTCCT GATTTGGGAA GTCCAACTTA CTTTGGCCAG ACAGCAGCTA 151 AGCTGGTTCA TCCCATCAGC CTGGATTGGT GAAACTGAAT CACAGGAGAT 201 ATTTCCAGGT TTGCTGGGAT GGGAAACCTG CTCAAAGTCC TTACCAGGGA 251 AATTGAAAAC TATCCACACT TTTTCCTGGA TTTTGAAAAT GCTCAGCCTA 301 CAGAAGGAGA GAGAGAAATC TGGAACCAGA TCAGCGCCGT CCTTCAGGAT 351 TCTGAGAGCA TCCTTGCAGA CCTGCAGGCT TACAAAGGCG CAGGCCCAGA 401 GATCCGAGAT GCAATTCAAA ATCCCAATGA CATTCAGCTT CAAGAAAAAG 451 CTTGGAATGC GGTGTGCCCT CTTGTTGTGA GGCTAAAGAG ATTTTACGAG 501 TTTTCCATTA GACTAGAAAA AGCTCTTCAG AGTTTATTGG AATCTCTGAC 551 TTGTCCACCC TACACACCAA CCCAACACCT GGAAAGGGAA CAGGCCCTGG 601 CAAAGGAGTT TGCCGAAATT TTACATTTTA CCCTTCGATT CGATGAGCTG 651 AAGATGAGGA ACCCGGCTAT TCAGAATGAC TTCAGCTACT ACAGAAGAAC 701 AATCAGTCGC AACCGCATCA ACAACATGCA CCTAGACATT GAGAATGAAG 751 TCAATAATGA GATGGCCAAT CGAATGTCCC TCTTCTATGC AGAAGCCACG 801 CCAATGCTGA AAACCCTTAG CAATGCCACA ATGCACTTTG TCTCTGAAAA 851 CAAAACTCTG CCAATAGAGA ACACCACAGA CTGCCTCAGC ACAATGACAA 901 GTGTCTGTAA AGTCATGCTG GAAACTCCGG AGTACAGAAG TAGGTTTACG 951 AGTGAAGAGA CCCTGATGTT CTGCATGAGG GTGATGGTGG GAGTCATCAT 1001 CCTCTATGAC CATGTCCACC CTGTGGGAGC TTTCTGCAAG ACATCCAAGA 1051 TCGATATGAA AGGCTGCATA AAAGTTTTGA AGGAGCAGGC CCCAGACAGT 1101 GTGGAGGGGC TGCTAAATGC CCTCAGGTTC ACTACAAAGC ACTTGAACGA 1151 TGAATCAACT TCCAAACAGA TTCGAGCAAT GCTTCAGTAG AGCTCTGCTC 1201 AAAGAAGAGG ATCTATGTGC TGACCTCAGA AGATGTATAT GTTTACATAA 1251 TTTAATACAG ATTGATGTTA ATACTTGTGT ATTTACATAA CCGTTTCCTT 1301 CTTGTCACTG AAATATATGG ACCTTAATTT GTATCCTGAC TGACTCAACC 1351 CAGCAGAGCA TAAATTGACT TGAGAGCCTT ACCTTTGATG TCTGAAATGA 1401 AACCCCCTTC TCCAAAGGCA AAATTCGGAG ACTTTGATCT TTGCTACTGG 1451 AGTCCTTTAA CAACATCTAT AACGATAAAA AATTCCTAAT TGTCAAAAAA 1501 AAAAAAAAAA AAA BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 3 ORF from 219 bp to 1187 bp; peptide length: 323 Category: similarity to unknown protein 1 MGNLLKVLTR EIENYPHFFL DFENAQPTEG EREIWNQISA VLQDSESILA 51 DLQAYKGAGP EIRDAIQNPN DIQLQEKAWN AVCPLVVRLK RFYEFSIRLE 101 KALQSLLESL TCPPYTPTQH LEREQALAKE FAEILHFTLR FDELKMRNPA 151 IQNDFSYYRR TISRNRINNM HLDIENEVNN EMANRMSLFY AEATPMLKTL 201 SNATMHFVSE NKTLPIENTT DCLSTMTSVC KVMLETPEYR SRFTSEETLM 251 FCMRVMVGVI ILYDHVHPVG AFCKTSKIDM KGCIKVLKEQ APDSVEGLLN 301 ALRFTTKHLN DESTSKQIRA MLQ BLASTP hits Entry CER07G3_7 from database TREMBL: gene: "R07G3.8"; Caenorhabditis elegans cosmid R07G3. Score = 544, P = 1.4e-52, identities = 119/323, positives 186/323 Alert BLASTP hits for DKFZphfkd2_24al5, frame 3 No Alert BLASTP hits found Pedant information for DKFZphfkd2_24al5, frame 3 Report for DKFZphfkd2_24al5.3 [LENGTH] 323 [MW] 37313.06 [pi] 5.71 [HOMOL] TREMBL :CER07G3 7 gene: "R07G3.8"; Caenorhabditis elegans cosmid R07G3. 4e-54 [PROSITE] MYRISTYL 1 [PROSITE] CK2_PHOSPHO_SITE 4 [PROSITE] TYR_PHOSPHO_SITE 1 [PROSITE] PKC_PHOSPHO_SITE 5 [PROSITE] ASN_GLYCOSYLATION 3 [KW] TRANSMEMBRANE 1 SEQ MGNLLKVLTREIENYPHFFLDFENAQPTEGEREIWNQISAVLQDSESILADLQAYKGAGP PRD ccccchhhhhhhhcccceeecccccccchhhhhhhhhhhhhhhcchhhhhhhhhhccccc MEM SEQ EIRDAIQNPNDIQLQEKAWNAVCPLVVRLKRFYEFSIRLEKALQSLLESLTCPPYTPTQH PRD hhhhhhccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccccccchhh MEM SEQ LEREQALAKEFAEILHFTLRFDELKMRNPAIQNDFSYYRRTISRNRINNMHLDIENEVNN PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccchhhhhhccchhhhhhhhhhhhhhhh MEM SEQ EMANRMSLFYAEATPMLKTLSNATMHFVSENKTLPIENTTDCLSTMTSVCKVMLETPEYR PRD hhhhhhhhhhhhccchhhhhhhhceeecccccccccccccceeeeehhhhhhhhcccccc MEM SEQ SRFTSEETLMFCMRVMVGVIILYDHVHPVGAFCKTSKIDMKGCIKVLKEQAPDSVEGLLN PRD cccccchhhhhhhhhhhheeeeeeeccccccccccccccchhhhhhhhhccccchhhhhh MEM MMMMMMMMMMMMMMMMMMMMM SEQ ALRFTTKHLNDESTSKQIRAMLQ PRD hhhhhhcccccccchhhhhhccc MEM Prosite for DKFZphfkd2_24al5.3 PS00001 202->206 ASN_GLYCOSYLATION PDOC00001 PS00001 211->215 ASN_GLYCOSYLATION PDOC00001 PS00001 218->222 ASN_GLYCOSYLATION PDOC00001 PS00005 96->99 PKC_PHOSPHO_SITE PDOC00005 PS00005 138->141 PKC_PHOSPHO_SITE PDOC00005 PS00005 275->278 PKC_PHOSPHO_SITE PDOC00005 PS00005 305->308 PKC PHOSPHO SITE PDOC00005 PS00005 314->317 PKC_PHOSPHO_SITE PDOC00005 PS00006 28->32 CK2_PHOSPHO_SITE PDOC00006 PS00006 105->109 CK2_PHOSPHO_SITE PDOC00006 PS00006 244->248 CK2_PHOSPHO_SITE PDOC00006 PS00006 276->280 CK2_PHOSPHO_SITE PDOC00006 PS00007 231->240 TYR_PHOSPHO_SITE PDOC00007 PS00008 297->303 MYRISTYL PDOC00008 (No Pfam data available for DKFZphf d2_24al5.3) DKFZphfkd2_24bl5 group: metabolism DKFZphfkd2_24bl5 encodes a novel 612 ammo acid protein with similarity to bacterial and yeast phosphoglucomutase and phosphomannomutases. The novel protein contains a phosphoserme signature typical for phosphoglucomutase (EC 5.4.2.2) or phosphomannomutase (EC 5.4.2.8). Thus, the protein seems to be taking part in the conversion of hexose phosphates. The new protein can find application in modulation of hexose metabolism pathways and as a new enzyme for biotechnologic production processes. similarity to phosphomannomutases complete cDNA, complete eds, EST hits potential start at bp 30 matches kozak consensus PyCNatgG, Sequenced by GBF Locus: map="158.8 cR from top of Chr4 linkage group" Insert length: 2204 bp Poly A stretch at pos. 2186, no polyadenylation signal found 1 GGGCTCTGCA GCGGTAGCAC AAGCTCAGCG ATGGCGGCTC CAGAAGGCAG 51 CGGTCTAGGC GAGGACGCCC GGCTGGACCA GGAGACCGCC CAGTGGCTGC 101 GCTGGGACAA GAATTCCTTA ACTTTGGAGG CAGTGAAACG ACTAATAGCA 151 GAAGGTAATA AAGAAGAACT ACGAAAATGT TTTGGGGCCC GAATGGAGTT 201 TGGGACAGCT GGCCTCCGAG CTGCTATGGG ACCTGGAATT TCTCGTATGA 251 ATGACTTGAC CATCATCCAG ACTACACAGG GATTTTGCAG ATACCTGGAA 301 AAACAATTCA GTGACTTAAA GCAGAAAGGC ATCGTGATCA GTTTTGACGC 351 CCGAGCTCAT CCATCCAGTG GGGGTAGCAG CAGAAGGTTT GCCCGACTTG 401 CTGCAACCAC ATTTATCAGT CAGGGGATTC CTGTGTACCT CTTTTCTGAT 451 ATAACGCCAA CCCCCTTTGT GCCCTTCACA GTATCACATT TGAAACTTTG 501 TGCTGGAATC ATGATAACTG CATCTCACAA TCCAAAGCAG GATAATGGTT 551 ATAAGGTCTA TTGGGATAAT GGAGCTCAGA TCATTTCTCC TCACGATAAA 601 GGGATTTCTC AAGCTATTGA AGAAAATCTA GAACCGTGGC CTCAAGCTTG 651 GGACGATTCT TTAATTGATA GCAGTCCACT TCTCCACAAT CCGAGTGCTT 701 CCATCAATAA TGACTACTTT GAAGACCTTA AAAAGTACTG TTTCCACAGG 751 AGCGTGAACA GGGAGACAAA GGTGAAGTTT GTGCACACCT CTGTCCATGG 801 GGTGGGTCAT AGCTTTGTGC AGTCAGCTTT CAAGGCTTTT GACCTTGTTC 851 CTCCTGAGGC TGTTCCTGAA CAGAGAGATC CGGATCCTGA GTTTCCAACA 901 GTGAAATACC CGAATCCCGA AGAGGGGAAA GGTGTCTTGA CTTTGTCTTT 951 TGCTTTGGCT GACAAAACCA AGGCCAGAAT TGTTTTAGCT AACGACCCGG 1001 ATGCTGATAG ACTTGCTGTG GCAGAAAAGC AAGACAGTGG TGAATGGAGG 1051 GTGTTTTCAG GCAATGAGTT GGGGGCCCTC CTGGGCTGGT GGCTTTTTAC 1101 ATCTTGGAAA GAGAAGAACC AGGATCGCAG TGCTCTCAAA GACACGTACA 1151 TGTTGTCCAG CACCGTCTCC TCCAAAATCT TGCGGGCCAT TGCCTTAAAG 1201 GAAGGTTTTC ATTTTGAGGA AACATTAACT GGCTTTAAGT GGATGGGAAA 1251 CAGAGCCAAA CAGCTAATAG ACCAGGGGAA AACTGTTTTA TTTGCATTTG 1301 AAGAAGCTAT TGGATACATG TGCTGCCCTT TTGTTCTGGA CAAAGATGGA 1351 GTCAGTGCCG CTGTCATAAG TGCAGAGTTG GCTAGCTTCC TAGCAACCAA 1401 GAATTTGTCT TTGTCTCAGC AACTAAAGGC CATTTATGTG GAGTATGGCT 1451 ACCATATTAC TAAAGCTTCC TATTTTATCT GCCATGATCA AGAAACCATT 1501 AAGAAATTAT TTGAAAACCT CAGAAACTAC GATGGAAAAA ATAATTATCC 1551 AAAAGCTTGT GGCAAATTTG AAATTTCTGC CATTAGGGAC CTTACAACTG 1601 GCTATGATGA TAGCCAACCT GATAAAAAAG CTGTTCTTCC CACTAGTAAA 1651 AGCAGCCAAA TGATCACCTT CACCTTTGCT AATGGAGGCG TGGCCACCAT 1701 GCGCACCAGT GGGACAGAGC CCAAAATCAA GTACTATGCA GAGCTGTGTG 1751 CCCCACCTGG GAACAGTGAT CCTGAGCAGC TGAAGAAGGA ACTGAATGAA 1801 CTGGTCAGTG CTATTGAAGA ACATTTTTTC CAGCCACAGA AGTACAATCT 1851 GCAGCCAAAA GCAGACTAAA ATAGTCCAGC CTTGGGTATA CTTGCATTTA 1901 CCTACAATTA AGCTGGGTTT AACTTGTTAA GCAATATTTT TAAGGGCCAA 1951 ATGATTCAAA ACATCACAGG TATTTATGTG TTTTACAAAG ACCTACATTC 2001 CTCATTGTTT CATGTTTGAC CTTTAAGGTG AAAAAAGAAA ATGGCCAAAC 2051 CCAACAAACT AACATTCCTA CTAAAAAGTT GAGCTTGGAC ATATTTTGAA 2101 TTTTTGTAAG TGAAGATTTT TAAACTGACT AACTTAAAAA AATAGATTGT 2151 AATTGATGTG CCTTAATTTG CATAAATCAT AAATGTAAAA AAAAAAAAAA 2201 AAAA BLAST Results Entry HS705145 from database EMBL: human STS WI-6820. Score = 1261, P = 3.6e-52, identities = 253/254 Medline entries No Medline entry Peptide information for frame 1 ORF from 31 bp to 1866 bp; peptide length: 612 Category: strong similarity to known protein 1 MAAPEGSGLG EDARLDQETA QWLRWDKNSL TLEAVKRLIA EGNKEELRKC 51 FGARMEFGTA GLRAAMGPGI SRMNDLTIIQ TTQGFCRYLE KQFSDLKQKG 101 IVISFDARAH PSSGGSSRRF ARLAATTFIS QGIPVYLFSD ITPTPFVPFT 151 VSHLKLCAGI MITASHNPKQ DNGYKVYWDN GAQIISPHDK GISQAIEENL 201 EPWPQAWDDS LIDSSPLLHN PSASINNDYF EDLKKYCFHR SVNRETKVKF 251 VHTSVHGVGH SFVQSAFKAF DLVPPEAVPE QRDPDPEFPT VKYPNPEEGK 301 GVLTLSFALA DKTKARIVLA NDPDADRLAV AEKQDSGEWR VFSGNELGAL 351 LGWWLFTSWK EKNQDRSALK DTYMLSSTVS SKILRAIALK EGFHFEETLT 401 GFKWMGNRAK QLIDQGKTVL FAFEEAIGYM CCPFVLDKDG VSAAVISAEL 451 ASFLATKNLS LSQQLKAIYV EYGYHITKAS YFICHDQETI KKLFENLRNY 501 DGKNNYPKAC GKFEISAIRD LTTGYDDSQP DKKAVLPTSK SSQMITFTFA 551 NGGVATMRTΞ GTEPKIKYYA ELCAPPGNSD PEQLKKELNE LVSAIEEHFF 601 QPQKYNLQPK AD BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfkd2_24bl5, frame 1 TREMBL :CEY43F4B_5 gene: "Y43F4B.5"; Caenorhabditis elegans cosmid Y43F4B, N = 1, Score = 1431, P = 1.6e-146 TREMBL :SPCC1840_5 gene: "SPCC1840.05c"; product: "similarity to phosphomannomutases"; S. pombe chromosome III cosmid cl840., N = 1, Score = 1210, P = 4.2e-123 PIR:S54585 hypothetical protein YMR278w - yeast (Saccharomyces cerevisiae), N = 1, Score = 1046, P = le-105 PIR:A71299 probable phosphomannomutase (manB) - syphilis spirochete, N = 1, Score = 697, P = 9.7e-69 >TREMBL:CEY43F4B_5 gene: "Y43F4B.5"; Caenorhabditis elegans cosmid Y43F4B Length = 595 HSPs: Score = 1431 (214.7 bits), Expect = 1.6e-146, P = 1.6e-146 Identities = 285/598 (47%), Positives = 393/598 (65%) Query: 13 ARLDQETAQWLRWDKNΞLTLEAVKRLIAEGNKEELRKCFGARMEFGTAGLRAAMGPGIΞR 72 A+LD++ A WL WDKN +++L+ E N + L+ R+ FGTAG+R+ M G R Sbjct: 6 AKLDKQVADWLAWDKNDKNRNEIQKLVDEKNVDALKARMDTRLVFGTAGVRSPMQAGFGR 65 Query: 73 MNDLTIIQTTQGFCRYLEKQFSDLKQKGIVISFDARAHPSSGGSSRRFARLAATTFISQG 132 +NDLTIIQ T GF R++ + K G+ I FD R + SRRFA L+A F+ Sbjct: 66 LNDLTIIQITHGFARHMLNVYGQPKN-GVAIGFDGRYN ΞRRFAELSANVFVRNN 118 Query: 133 IPVYLFSDITPTPFVPFTVSHLKLCAGIMITAΞHNPKQDNGYKVYWDNGAQIISPHDKGI 192 IPVYLFS+++PTP V + L AG++ITASHNPK+DNGYK YW NGAQII PHD I Sbjct: 119 IPVYLFSEVSPTPVVSWATIKLGCDAGLIITASHNPKEDNGYKAYWSNGAQIIGPHDTEI 178 Query: 193 SQAIEENLEPWPQAWDDSLIDSSPLLHNPSASINNDYFEDLKKYCFHRSVNRETKVKFVH 252 + E +P + WD S + SSPL H+ 1+ YFE K F R +N T +KF + Sbjct: 179 VRIKEAEPQPRDEYWDLSELKSSPLFHSADVVID-PYFEVEKSLNFTREINGSTPLKFTY 237 Query: 253 TSVHGVGHSFVQSAFKAFDLVPPE—AVPEQRDPDPEFPTVKYPNPEEGKGVLTLSFALA 310 ++ HG+G+ + + F F +V EQ+DP+P+FPT+ +PNPEEG+ VLTL+ A Sbjct: 238 SAFHGIGYHYTKRMFAEFGFPASΞFISVAEQQDPNPDFPTIPFPNPEEGRKVLTLAMETA 297 Query: 311 DKTKARIVLANDPDADRLAVAEKQDSGEWRVFSGNELGALLGWWLFTSWKEKNQDRSALK 370 DK + ++LANDPDADR+ +AEKQ GEWRVF+GNE+GAL+ WW++T+W++ N + A K Sbjct: 298 DKNGSTVILANDPDADRIQMAEKQKDGEWRVFTGNEMGALITWWIWTNWRKANPNADASK 357 Query: 371 DTYMLSSTVSSKILRAIALKEGFHFEETLTGFKWMGNRAKQLIDQGKTVLFAFEEAIGYM 430 Y+L+S VSS+I++ IA EGF E TLTGFKWMGNRA++L G V+ A+EE+IGYM Sbjct: 358 -VYILNSAVSSQIVKTIADAEGFKNETTLTGFKWMGNRAEELRADGNQVILAWEESIGYM 416 Query: 431 CCP-FVLDKDGVSAAVISAELASFLATKNLSLSQQLKAIYVEYGYHITKASYFICHDQET 489 P +DKDGVSAA + AE+A+FL + SL QL A+Y YG+H+ +++Y++ E Sbjct: 417 —PGHTMDKDGVSAAAVFAEIAAFLHAEGKSLQDQLYALYNRYGFHLVRSTYWMVPAPEV 474 Query: 490 IKKLFENLRNYDGKNNYPKACGKFEISAIRDLTTGYDDSQPDKKAVLPTSKSSQMITFTF 549 KKLF LR D K +P G+ E++++RDLT GYD+S+PD K VLP S SS+M+TF Sbjct: 475 TKKLFSTLRA-DLK—FPTKIGEAEVASVRDLTIGYDNSKPDNKPVLPLSTSSEMVTFFL 531 Query: 550 ANGGVATMRTSGTEPKIKYYAELCAPPGNS—DPEQLKKELNELVSAIEEHFFQPQKYNL 607 G V T+R SGTEPKIKYY EL PG + D E + E+++L + +PQ++ L Sbjct: 532 KTGSVTTLRASGTEPKIKYYIELITAPGKTQNDLESVISEMDQLEKDVVATLLRPQQFGL 591 Query: 608 QPK 610 P+ Sbjct: 592 IPR 594 Pedant information for DKFZphfkd2_24bl5, frame 1 Report for DKFZphfkd2_24bl5.1 [LENGTH] 612 [MW] 68311.58 [pi] 6.28 [HOMOL] TREMBL :CEY43F4B_5 gene: "Y43F4B.5"; Caenorhabditis elegans cosmid Y43F4B le-157 [FUNCAT] 01.05.01 carbohydrate utilization [S. cerevisiae, YMR278w] le-111 [FUNCAT] g carbohydrate metabolism and transport [H. influenzae, HI0740] 3e-66 [FUNCAT] c energy conversion [M. genitalium, MG053] 4e-50 [FUNCAT] m outer membrane and cell wall [H. influenzae, HI1463] 2e-04 [BLOCKS] BL00607D cAMP phosphodiesterases class-II proteins [BLOCKS] BL00710 Phosphoglucomutase and phosphomannomutase phosphoserme signa [EC] 5.4.2.8 Phosphomannomutase 3e-56 [EC] 5.4.2.2 Phosphoglucomutase le-09 [PIRKW] lsomerase 3e-56 [PIRKW] intramolecular transferase 3e-56 [SUPFAM] Methanobacteπum thermoautotrophicum phosphomannomutase le-06 [SUPFAM] probable phosphorylating protein ureC 9e-06 [PROSITE] PGM_PMM 1 [PROSITE] MYRISTYL 10 [PROSITE] LIPOCALIN 2 [PROSITE] CK2_PHOSPHO_SITE 9 [PROSITE] GLYCOSAMINOGLYCAN 1 [PROSITE] PKC_PHOSPHO_SITE 8 [PROSITE] ASN_GLYCOSYLATION 1 [PFAM] Phosphoglucomutase and phosphomannomutase phosphoserme [KW] Alpha_Beta SEQ MAAPEGSGLGEDARLDQETAQWLRWDKNΞLTLEAVKRLIAEGNKEELRKCFGARMEFGTA PRD ccccccccccchhhhhhhhhhhhhhhccchhhhhhhhhhhhcchhhhhhhhhhhhccccc SEQ GLRAAMGPGISRMNDLTIIQTTQGFCRYLEKQFSDLKQKGIVISFDARAHPSSGGSSRRF PRD cccccccccccccceeeeeehhhhhhhhhhhhcccccceeeeeecccccccccccchhhh SEQ ARLAATTFISQGIPVYLFSDITPTPFVPFTVSHLKLCAGIMITASHNPKQDNGYKVYWDN PRD hhhhhhhhhhccceeeeeccccccccchhhhhhhcccceeeeeeccccccccceeeeecc SEQ GAQIISPHDKGISQAIEENLEPWPQAWDDSLIDSSPLLHNPSASINNDYFEDLKKYCFHR PRD ccccccccchhhhhhhhhhhhhhcccccccccccccccccccchhhhhhhhhhhhhhhcc SEQ SVNRETKVKFVHTSVHGVGHSFVQSAFKAFDLVPPEAVPEQRDPDPEFPTVKYPNPEEGK PRD ccccccceeeeeeeccccccchhhhhhhhhcccccccccccccccccccccccccccchh SEQ GVLTLSFALADKTKARIVLANDPDADRLAVAEKQDSGEWRVFSGNELGALLGWWLFTSWK PRD hhhhhhhhhhhhhcceeeeeccccccceeeeecccccceeeecccchhhhhhhhhhhhhh SEQ EKNQDRSALKDTYMLSSTVSSKILRAIALKEGFHFEETLTGFKWMGNRAKQLIDQGKTVL PRD hcccccccccceeeeeeeehhhhhhhhhhhcccceeeeeccccchhhhhhhhhhccceee SEQ FAFEEAIGYMCCPFVLDKDGVSAAVISAELASFLATKNLSLSQQLKAIYVEYGYHITKAS PRD hhhhhccccccccccccccchhhhhhhhhhhhhhhhhccchhhhhhhhhhhhcccccccc SEQ YFICHDQETIKKLFENLRNYDGKNNYPKACGKFEISAIRDLTTGYDDSQPDKKAVLPTSK PRD eeeccchhhhhhhhhhhhhhhcccccccccchhhhhhhcccccccccccccccccccccc SEQ SSQMITFTFANGGVATMRTSGTEPKIKYYAELCAPPGNSDPEQLKKELNELVSAIEEHFF PRD ccceeeeeecccceeeeecccccccceeeeeeccccccchhhhhhhhhhhhhhhhhhhhh SEQ QPQKYNLQPKAD PRD cccccccccccc Prosite for DKFZphfkd2_24bl5.1 PS00001 458->462 ASNJ3LYCOSYLATION PDOC00001 PS00002 7->ll GLYCOSAMINOGLYCAN PDOC00002 PS00005 116->119 PKC_PHOSPHO_SITE PDOC00005 PS00005 117->120 PKC_PHOΞPHO_SITE PDOC00005 PS00005 290->293 PKC_PHOSPHO_SITE PDOC00005 PS00005 358->361 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 380->383 PKC_PHOSPHO_SITE PDOC00005 PS00005 489->492 PKC_PHOSPHO_SITE PDOC00005 PS00005 538->541 PKC_PHOΞPHO_SITE PDOC00005 PS00005 556->559 PKC_PHOSPHO_SITE PDOC00005 PS00006 186->190 CK2_PHOSPHO_SITE PDOC00006 PS00006 210->214 CK2_PHOSPHO_SITE PDOC00006 PS00006 343->347 CK2_PHOSPHO_SITE PDOC00006 PS00006 358->362 CK2_PHOSPHO_SITE PDOC00006 PS00006 523->527 CK2_PHOSPHO_SITE PDOC00006 PS00006 528->532 CK2_PHOSPHO_SITE PDOC00006 PS00006 560->564 CK2_PHOSPHO_SITE PDOC00006 PS00006 579->583 CK2_PHOSPHO_SITE PDOC00006 PS00006 593->597 CK2_PHOSPHO_SITE PDOC00006 PS00008 6->12 MYRISTYL PDOC00008 PS00008 61->67 MYRISTYL PDOC00008 PS00008 100->106 MYRISTYL PDOC00008 PS00008 159->165 MYRISTYL PDOC00008 PS00008 191-M97 MYRISTYL PDOC00008 PS00008 257->263 MYRISTYL PDOC00008 PS00008 344->350 MYRISTYL PDOC00008 PS00008 348->354 MYRISTYL PDOC00008 PS00008 440->446 MYRISTYL PDOC00008 PS00008 552->558 MYRISTYL PDOC00008 PS00710 159->174 PGM_PMM PDOC00589 PS00213 346->358 LIPOCALIN PDOC00187 PS00213 344->358 LIPOCALIN PDOC00187 Pfam for DKFZphf d2_24bl5.1 HMM_NAME Phosphoglucomutase and phosphomannomutase phosphoserme HMM *GvnVIdIGQNGMMPTPMIYFaIRTYKhmcmggGIMITaSHNPGGPDnDN G+ V + ++PTP + F + H+++ +GIMITASHNP DN Query 132 GIPVYLFS--DITPTPFVPFTVS HLKLCAGIMITASHNP—KQ-DN 172 HMM GIK* G+K Query 173 GYK 175 DKFZphfkd2_24e23 group: kidney derived DKFZphfkd2_24e23 encodes a novel 198 amino acid protein without similarity to known proteins. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application m studying the expression profile of kidney-specific genes. unknown complete cDNA, complete eds, 1 EST hit, many ATGs in front of the ORF Sequenced by GBF Locus : unknown Insert length: 1723 bp Poly A stretch at pos. 1695, no polyadenylation signal found 1 GGGGGATTTT CGATCATGAC AACGATAGCA ATTGATATAC CTTCAAAATA 51 CGTGTCCAGT GAGTGTTGAT TGTGTGTGGT TTCTCTAGGA GACCGTGTTC 101 ATGCAACACA GCATTATTTC ACCGCCTTTA CCCCAGCTTC TTCATACACA 151 TGCACTTGTC AAGGGCTCTT TGGCTGAAGA GAAGTTAGAA GTTTCCAGAT 201 ATGGAGGGGT ATTTTCAGCA GATATGCCCA CCGCCATGGT TTTGTCAGCT 251 CTGTAGGGTG GTCTTGCACC CTGCTCACTG CTGGCATCAC CTGAGCCTAT 301 GGCAGATACC CAGTGCTGCC CGCCACCATG TGAATTCATC AGCTCTGCAG 351 GCACAGACCT TGCACTAGGA ATGGGCTGGG ACGCCACCCT CTGCCTCTTA 401 CCATTCACTG GGTTTGGCAA GTGTGCTGGG ATCTGGAATC ACATGGATGA 451 GGAACCCGAT AATGGTGACG ACCGAGGTAG CAGGCGAACC ACTGGCCAGG 501 GCAGGAAGTG GGCAGCTCAC GGGACTATGG CTGCACCGCG GGTTCATACC 551 GACTACCATC CTGGAGGTGG GAGCGCATGC TCATCTGTAA AAGTCCGGTC 601 CCACGTTGGA CACACCGGGG TCTTCTTCTT TGTTGACCAG GATCCTCTGG 651 CAGTGTCTTT AACAAGCCAG AGTCTGATCC CACCGCTCAT AAAGCCAGGG 701 TTGTTGAAAG CTTGGGGCTT CCTCCTCCTC TGTGCGCAGC CCTCAGCAAA 751 CGGTCACAGC CTGTGCTGTC TGCTGTACAC CGACTTGGTA TCATCCCATG 801 AACTGTCCCC CTTTCGTGCT CTGTGCTTAG GGCCCTCTGA TGCCCCATCT 851 GCCTGCGCTT CCTGCAACTG TTTAGCAAGC ACCTATTATC TATAGGGTGC 901 TGGGGTGCTG GGCGAGGCCA ATCGCTCCTA TTACTTTCTG CCCTGGGGAC 951 GTCCTGTTTT CCCACCTACC CCTGTAACGC CTCTGCTCTG CCTTCCCATC 1001 TGCGGGGCTA ACGCCATCCC ACAAGGGCTG GGCTGTCCGT TCAGAAGAGA 1051 AACTGGGAAG GGGCCTTGAG GACCTGTGTC CAGGCAGGGT GGACAAGGGC 1101 TTTGTGCAGG GAGCTCCTCT CCCATCTTTG TGTCCTGACA GCCGTGACCG 1151 TGACCCCTCA AAGCAGAGCC AGTAGTGATC AGTATCCTGC TGCTTCAAGC 1201 CTGCACGGTC CTCTTCTCCT CTCCGCACAT CTGCATGCCT GTCAAACCCA 1251 GAGTAGTTTG GGGCCTGGTA AACAGAGGGA AGTTGGCTGG AGGAGGCCAG 1301 TCAGGAGTGC AAGAACCCCG CGTACTCTGT CCCACGTGGA TAAAGTCTCT 1351 AATTCCAGTC TGAGGTGAAT TCTTAGAGAG TGCTTTCATT TAATGTTTGC 1401 TTTATGCATT TCCCCTGCAG CTGTGACTAA TTGTGGAACA GCATACATTT 1451 TGTTTTGAGA CTCTCTTGAG ATTTTTCTGG CAGTGTAAGG TCTACACCAT 1501 TTTCCTCTCA GCATCAGAGA AGGCAGAAAG CAAGAGAAAG GAATGCAATG 1551 TGAGCAAGGC CAGGCACACT TGTGCTACTG CAGTTGGCAA GAATGGAGTC 1601 TAATCCCAGC ACTTTGGGAG GCCGAGGCGG GTGGATCACC TGAGGTCAGG 1651 AATTTGAGAC CAACCTGGCC AACATGTTGA AACCTCGTCT GTACTAAAAA 1701 TACAAAAAAA AAAAAAAAAA AAA BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 2 ORF from 299 bp to 892 bp; peptide length: 198 Category: putative protein 1 MADTQCCPPP CEFISSAGTD LALGMGWDAT LCLLPFTGFG KCAGIWNHMD 51 EEPDNGDDRG SRRTTGQGRK WAAHGTMAAP RVHTDYHPGG GSACSSVKVR 101 SHVGHTGVFF FVDQDPLAVS LTSQSLIPPL IKPGLLKAWG FLLLCAQPSA 151 NGHSLCCLLY TDLVSSHELS PFRALCLGPS DAPSACASCN CLASTYYL BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfkd2_24e23, frame 2 No Alert BLASTP hits found Pedant information for DKFZphfkd2_24e23, frame 2 Report for DKFZphfkd2 24e23.2 [LENGTH] 198 [MW] 20948.98 [pi] 6.01 [PROSITE] MYRISTYL 5 [PROSITE] AMIDATION 1 [PROSITE] CAMP PHOSPHO SITE [PROSITE] CK2 PHOSPHO SITE [PROSITE] PKC PHOSPHO SITE [KW] All Beta [KW] LOW COMPLEXITY 6.06 % SEQ MADTQCCPPPCEFISSAGTDLALGMGWDATLCLLPFTGFGKCAGIWNHMDEEPDNGDDRG SEG PRD ccccccccccccccccccccccccccccceeeeeccccccceeeeccccccccccccccc SEQ SRRTTGQGRKWAAHGTMAAPRVHTDYHPGGGSACSSVKVRSHVGHTGVFFFVDQDPLAVS SEG PRD cccccccccccccccccccceeeeecccccccccceeeeeeeccccceeeeeccccceee SEQ LTSQSLIPPLIKPGLLKAWGFLLLCAQPSANGHSLCCLLYTDLVSSHELSPFRALCLGPS SEG xxxxxxxxxxxx PRD eccccccccccccchhhhhhhhhhhccccccccceeeeeeeeeccccccccceeeecccc SEQ DAPSACASCNCLASTYYL SEG PRD cccccccccccccccccc Prosite for DKFZphf d2_24e23.2 PS00004 62->66 CAMP_PHOSPHO_SITE PDOC00004 PS00005 61->64 PKC_PHOSPHO_SITE PDOC00005 PS00005 96->99 PKC_PHOSPHO_SITE PDOC00005 PS00006 165->169 CK2_PHOSPHO_SITE PDOC00006 PS00008 18->24 MYRISTYL PDOC00008 PS00008 60->66 MYRISTYL PDOC00008 PS00008 89->95 MYRISTYL PDOC00008 PS00008 91->97 MYRISTYL PDOC00008 PS00008 134->140 MYRISTYL PDOC00008 PS00009 67->71 AMIDATION PDOC00009 (No Pfam data available for DKFZphfkd2 24e23.2) DKFZphfkd2_24n20 group: intracellular transport and trafficking DKFZphf d2_24n20.3 encodes a novel 366 amino acid protein with similarity to human epsδ binding protein e3Bl and spectrins . The new protein contains an Src homology domain 3 and is similar to human eps8 SH3 domain binding protein 1 (e3Bl) and spectrins. Eps8 is a substrate of receptor tyrosine kinases involved in mitogenic signaling. Spectrin is part of the submembrane cytoskeletal network in the human erythrocyte ghost. Nonerythroid spectrins are proposed to have roles in cell adhesion, establishment of cell polarity, and attachment of other cytoskeletal structures to the plasma membrane. The new protein seems to be part of the signalling pathway between tyrosine kinases and the membrane/cyto skeleton. The new protein can find application in modulating cell adhesion/motility and membrane/cyto skeleton structure and dynamics. strong similarity to eps8 binding protein e3Bl complete cDNA, complete eds, few EST hits potential start at Bp 300, but there are ATGs in other frames in 5 ' region of the cDNA Sequenced by GBF Locus: /map="17" Insert length: 1719 bp Poly A stretch at pos. 1699, polyadenylation signal at pos. 1680 1 GGGGACAGCT GCCCCGACCT TGGCTTCCTC TGCTGGGTGG GATTGGGGGC 51 TGGGCCCCCA AATGGGCCCC TGGCTTCCCC CTTCCTCTGG GCAGGGGACA 101 GAGAGACACA GGCTCGGGGA GCAGGACTGA CTTCCTCTTG TCCCGGAATG 151 AGCATGCCTG CCCTTTGCAA GCAGGTTTGG GTCTCACGCA GAGGAAACCA 201 AAAGCAATAA GAGGGAGGGA AGGCAGAGCA ACCAATCAAG GGCAGGGTGA 251 GACTCAAAAC GAGCGGGCTC CCTGGGGAGC CAGACAGAGG CTGGGGGTGA 301 TGGCGGAGCT ACAGCAGCTG CAGGAGTTTG AGATCCCCAC TGGCCGGGAG 351 GCTCTGAGGG GCAACCACAG TGCCCTGCTG CGGGTCGCTG ACTACTGCGA 401 GGACAACTAT GTGCAGGCCA CAGACAAGCA GAAGGCGCTG GAGGAGACCA 451 TGGCCTTCAC TACCCAGGCA CTGGCCAGCG TGGCCTACCA GGTGGGCAAC 501 CTGGCCGGGC ACACTCTGCG CATGTTGGAC CTGCAGGGGG CCGCCCTGCG 551 GCAGGTGGAA GCCCGTGTAA GCACGCTGGG CCAGATGGTG AACATGCATA 601 TGGAGAAGGT GGCCCGAAGG GAGATCGGCA CCTTAGCCAC TGTCCAGCGG 651 CTGCCCCCCG GCCAGAAGGT CATCGCCCCA GAGAACCTAC CCCCTCTCAC 701 GCCCTACTGC AGGAGACCCC TCAACTTTGG CTGCCTGGAC GACATTGGCC 751 ATGGGATCAA GGACCTCAGC ACGCAGCTGT CAAGAACAGG CACCCTGTCT 801 CGAAAGAGCA TCAAGGCCCC TGCCACACCC GCCTCCGCCA CCTTGGGGAG 851 ACCGCCCCGG ATTCCCGAGC CAGTGCACCT GCCGGTGGTG CCCGACGGCA 901 GACTCTCCGC CGCCTCCTCT GCGTCTTCCC TGGCCTCGGC CGGCAGCGCC 951 GAAGGTGTCG GTGGGGCCCC CACGCCCAAG GGGCAGGCAG CACCTCCAGC 1001 CCCACCTCTC CCCAGCTCCT TGGACCCACC TCCTCCACCA GCAGCCGTCG 1051 AGGTGTTCCA GCGGCCTCCC ACGCTGGAGG AGTTGTCCCC ACCCCCACCG 1101 GACGAAGAGC TGCCCCTGCC ACTGGACCTG CCTCCTCCTC CACCCCTGGA 1151 TGGAGATGAA TTGGGGCTGC CTCCACCCCC ACCAGGATTT GGGCCTGATG 1201 AGCCCAGCTG GGTGCCTGCC TCATACTTGG AGAAAGTGGT GACACTGTAC 1251 CCATACACCA GCCAGAAGGA CAATGAGCTC TCCTTCTCTG AGGGCACTGT 1301 CATCTGTGTC ACTCGCCGCT ACTCCGATGG CTGGTGCGAG GGCGTCAGCT 1351 CGGAGGGGAC TGGATTCTTC CCTGGGAACT ATGTGGAGCC CAGCTGCTGA 1401 CAGCCCAGGG CTCTCTGGGC AGCTGATGTC TGCACTGAGT GGGTTTCATG 1451 AGCCCCAAGC CAAAACCAGC TCCAGTCACA GCTGGACTGG GTCTGCCCAC 1501 CTCTTGGGCT GTGAGCTGTG TTCTGTCCTT CCTCCCATCG GAGGGAGAAG 1551 GGGTCCTGGG GAGAGAGAAT TTATCCAGAG GCCTGCTGCA GATGGGGAAG 1601 AGCTGGAAAC CAAGAAGTTT GTCAACAGAG GACCCCTACT CCATGCAGGA 1651 CAGGGTCTCC TGCTGCAAGT CCCAACTTTG AATAAAACAG ATGATGTCCA 1701 AAAAAAAAAA AAAAAAAAA BLAST Results Entry AC004797 from database EMBL: Homo sapiens chromosome 17, clone hRPC.62_0_9, complete sequence. Score = 2316, P = 5.9e-255, identities = 464/465 7 exons Bp 93317-110902 Medline entries 97163405: Isolation and characterization of e3Bl, an eps8 binding protein that regulates cell growth. 98256293: Identification of a candidate human spectrin Src homology 3 domain-binding protein suggests a general mechanism of association of tyrosine kinases with the spectrm-based membrane skeleton. Peptide information for frame 3 ORF from 300 bp to 1397 bp; peptide length: 366 Category: strong similarity to known protein 1 MAELQQLQEF EIPTGREALR GNHSALLRVA DYCEDNYVQA TDKQKALEET 51 MAFTTQALAS VAYQVGNLAG HTLRMLDLQG AALRQVEARV STLGQMVNMH 101 MEKVARREIG TLATVQRLPP GQKVIAPENL PPLTPYCRRP LNFGCLDDIG 151 HGIKDLSTQL SRTGTLSRKS IKAPATPASA TLGRPPRIPE PVHLPVVPDG 201 RLSAASSASS LASAGSAEGV GGAPTPKGQA APPAPPLPSS LDPPPPPAAV 251 EVFQRPPTLE ELSPPPPDEE LPLPLDLPPP PPLDGDELGL PPPPPGFGPD 301 EPSWVPASYL EKVVTLYPYT SQKDNELSFS EGTVICVTRR YSDGWCEGVS 351 SEGTGFFPGN YVEPSC BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfkd2_24n20, frame 3 No Alert BLASTP hits found Pedant information for DKFZphf d2_24n20, frame 3 Report for DKFZphfkd2_24n20.3 [LENGTH] 366 [MW] 38947.21 [pi] 4.93 [HOMOL] TREMBL:U87166 1 gene: "SSH3BP1"; product: "spectrm SH3 domain binding protein Homo sapiens spectrm SH3 domain binding protein 1 (SSH3BP1) mRNA, complete eds. 3e-48 [FUNCAT] 10.99 other signal-transduction activities [S. cerevisiae, YGR136w] 9e-06 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YGR136w] 9e-06 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YPR154w] 3e-05 [FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YDR388w] 2e-04 [FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YDR388w] 2e-04 [FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YDR162c] 4e-04 [BLOCKS] BL50002B Src homology 3 (SH3) domain proteins profile [SUPFAM] SH3 homology 6e-17 [PROSITE] MYRISTYL 6 [PROSITE] CAMP_PHOSPHO_SITE 1 [PROSITE] CK2_PHOΞPHO_SITE 6 [PROSITE] PKC_PHOSPHO_SITE 8 [PROSITE] ASN_GLYCOSYLATION 1 [PFAM] Src homology domain 3 [KW] Irregular [KW] 3D [KW] LOW COMPLEXITY 24.04 % SEQ MAELQQLQEFEIPTGREALRGNHSALLRVADYCEDNYVQATDKQKALEETMAFTTQALAS SEG laboA SEQ VAYQVGNLAGHTLRMLDLQGAALRQVEARVSTLGQMVNMHMEKVARREIGTLATVQRLPP SEG laboA SEQ GQKVIAPENLPPLTPYCRRPLNFGCLDDIGHGIKDLSTQLSRTGTLSRKSIKAPATPASA SEG laboA SEQ TLGRPPRIPEPVHLPVVPDGRLSAASSASSLASAGSAEGVGGAPTPKGQAAPPAPPLPSS SEG xxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxx laboA SEQ LDPPPPPAAVEVFQRPPTLEELSPPPPDEELPLPLDLPPPPPLDGDELGLPPPPPGFGPD SEG xxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx laboA SEQ EPSWVPASYLEKVVTLYPYTSQKDNELSFSEGTVICVTRRYSDGWCEGVSSEGTGFFPGN SEG xx laboA EECCCBCCCTTTBCCBTTTEEEEEEEETTTTEEEEEETTEEEEEEGG SEQ YVEPSC SEG laboA GEEE.. Prosite for DKFZphfkd2_24n20.3 PS00001 22->26 ASN_GLYCOSYLATION PDOC00001 PS00004 339->343 CAMP_PHOSPHO_SITE PDOC00004 PS00005 14->17 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 41->44 PKC_PHOSPHO_SITE PDOC00005 PS00005 72->75 PKC_PHOSPHO_SITE PDOC00005 PS00005 167->170 PKC_PHOSPHO_SITE PDOC00005 PS00005 170->173 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 225->228 PKC_PHOSPHO_SITE PDOC00005 PS00005 321->324 PKC_PHOSPHO_SITE PDOC00005 PS00005 338->341 PKC_PHOSPHO_SITE PDOC00005 PS00006 14->18 CK2_PHOSPHO_SITE PDOC00006 PS00006 239->243 CK2_PHOSPHO_SITE PDOC00006 PS00006 258->262 CK2_PHOSPHO_SITE PDOC00006 PS00006 308->312 CK2_PHOSPHO_SITE PDOC00006 PS00006 321->325 CK2_PHOSPHO_SITE PDOC00006 PS00006 328->332 CK2_PHOSPHO_SITE PDOC00006 PS00008 21->27 MYRISTYL PDOC00008 PS00008 66->72 MYRISTYL PDOC00008 PS00008 94->100 MYRISTYL PDOC00008 PS00008 110->116 MYRISTYL PDOC00008 PS00008 215->221 MYRISTYL PDOC00008 PS00008 332->338 MYRISTYL PDOC00008 Pfam for DKFZphf d2_24n20.3 HMM_NAME Src homology domain 3 HMM *pyVIALYDYqAqdpDELSFkEGDIIιIIEdsDD.WWrgRnnnTNGQEGW ++V+ LY+Y++Q ++ELSF EG +1 + + D W++G + +G+ Query 311 EKVVTLYPYTSQKDNELSFSEGTVICVTRRYSDGWCEGVSSE GTGF 356 HMM IPSNYVEPi* +P NYVEP Query 357 FPGNYVEPS 365 DKFZphfkd2_24p5 group: intracellular transport and trafficking DKFZphfkd2_24p5 encodes a novel 811 amino acid protein which is a novel splice variant of human ankyrin G. The ankyrin 3 gene encodes a novel ankyrin, which is expressed m multiple tissues, with very high expression at the axonal initial segment and nodes of Ranvier of neurons in the central and peripheral nervous systems. Ankyrin G shows several tissue-specific alternative mRNA processing. The different ankyrin G proteins participate in maintenance/targeting of ion channels and cell adhesion molecules to nodes of Ranvier and axonal initial segments. The new protein can find application in modulating the structure and membrane topology of Ranvier nodes and other neuronal cell membranes. Human ankyrin G (ANK-3) new splice variant splice variant potential frame shift at 2720 was checked see BLASTX Sequenced by EMBL Locus: /map="10q21" Insert length: 3470 bp Poly A stretch at pos. 3459, no polyadenylation signal found 1 AGCTTTAAAA GGATGTCTGC GAAGTGGTCA AAAGGATCTT AACCTCAATT 51 AAGTGGGGTT TTTTAAAAAG ATTTTTTGGG GGGCCTGAAA TTTTGAAAAT 101 CTTCGAACTC TGAGTGGGGA AAGATGTATA ATTCCTCAAT TGCCTACGAG 151 GATATCAAGA TGCTGAGAGG AATTCAGCGG TGGTGAAGAG AGTGGATACA 201 AACCAGGGAT TGGTTTCCTT GAGCTGTTTT GGAGGTTGAT TCTAAATCAC 251 TGCTTAAGGA ATTCCTGGAA ACATCAGGAA AACATTTGAT CATCCAAGCC 301 TAGTGGAAAT GGCTTTACCG CAGAGTGAAG ATGCAATGAC CGGGGACACA 351 GACAAATATC TTGGGCCACA GGACCTTAAG GAATTGGGTG ATGATTCCCT 401 GCCTGCAGAG GGTTACATGG GCTTTAGTCT CGGAGCGCGT TCTGCCAGCC 451 TCCGCTCCTT CAGTTCGGAT GGGTCTTACA CCTTGAACAG AAGCTCCTAT 501 GCACGGGACA GCATGATGAT TGAAGAACTC CTCGTGCCAT CCAAAGAGCA 551 GCATCTAACA TTCACAAGGG AATTTGATTC AGATTCTCTT AGACATTACA 601 GCTGGGCTGC AGACACCTTA GACAATGTCA ATCTTGTTCC AAGCCCCATT 651 CATTCTGGGT TTCTGGTTAG CTTTATGGTG GACGCGAGAG GGGGCTCCAT 701 GAGAGGAAGC CGTCATCACG GGATGAGAAT CATCATTCCT CCACGCAAGT 751 GTACGGCCCC CACTCGAATC ACCTGCCGTT TGGTAAAGAG ACATAAACTG 801 GCCAACCCAC CCCCCATGGT GGAAGGAGAG GGATTAGCCA GTAGGCTGGT 851 AGAAATGGGT CCTGCAGGGG CACAATTTTT AGGCCCTGTC ATAGTGGAAA 901 TCCCTCACTT TGGGTCCATG AGAGGAAAAG AGAGAGAACT CATTGTTCTT 951 CGAAGTGAAA ATGGTGAAAC TTGGAAGGAG CATCAGTTTG ACAGCAAAAA 1001 TGAAGATTTA ACCGAGTTAC TTAATGGCAT GGATGAAGAA CTTGATAGCC 1051 CAGAAGAGTT AGGGAAAAAG CGTATCTGCA GGATTATCAC GAAAGATTTC 1101 CCCCAGTATT TTGCAGTGGT TTCCCGGATT AAGCAGGAAA GCAACCAGAT 1151 TGGTCCTGAA GGTGGAATTC TGAGCAGCAC CACAGTGCCC CTTGTTCAAG 1201 CATCTTTCCC AGAGGGTGCC CTAACTAAAA GAATTCGAGT GGGCCTCCAG 1251 GCCCAGCCTG TTCCAGATGA AATTGTGAAA AAGATCCTTG GAAACAAAGC 1301 AACTTTTAGC CCAATTGTCA CTGTGGAACC AAGAAGACGG AAATTCCATA 1351 AACCAATCAC AATGACCATT CCGGTGCCCC CGCCCTCAGG AGAAGGTGTA 1401 TCCAATGGAT ACAAAGGGGA CACTACACCC AATCTGCGTC TTCTCTGTAG 1451 CATTACAGGG GGCACTTCGC CTGCTCAGTG GGAAGACATC ACAGGAACAA 1501 CTCCTTTGAC GTTTATAAAA GATTGTGTCT CCTTTACAAC CAATGTTTCA 1551 GCCAGATTTT GGCTTGCAGA CTGCCATCAA GTTTTAGAAA CTGTGGGGTT 1601 AGCCACGCAA CTGTACAGAG AATTGATATG TGTTCCATAT ATGGCCAAGT 1651 TTGTTGTTTT TGCCAAAATG AATGATCCCG TAGAATCTTC CTTGCGATGT 1701 TTCTGCATGA CAGATGACAA AGTGGACAAA ACTTTAGAGC AACAAGAGAA 1751 TTTTGAGGAA GTCGCAAGAA GCAAAGATAT TGAGGTTCTG GAAGGAAAAC 1801 CTATTTATGT TGATTGTTAT GGAAATTTGG CCCCACTTAC CAAAGGAGGA 1851 CAGCAACTTG TTTTTAACTT TTATTCTTTC AAAGAAAATA GACTGCCATT 1901 TTCCATCAAG ATTAGAGACA CCAGCCAAGA GCCCTGTGGT CGTCTGTCTT 1951 TTCTGAAAGA ACCAAAGACA ACAAAAGGAC TGCCTCAAAC AGCGGTTTGC 2001 AACTTAAATA TCACTCTGCC AGCACATAAA AAGATTGAGA AAACAGATGG 2051 ACGACAGAGC TTCGCATCCT TAGCTTTACG TAAGCGCTAC AGCTACTTGA 2101 CTGAGCCTGG AATGAGTCCA CAGAGTCCAT GTGAACGGAC AGATATCAGG 2151 ATGGCAATAG TAGCCGATCA CCTGGGACTT AGTTGGACAG AACTGGCAAG 2201 GGAACTGAAT TTTTCAGTGG ATGAAATCAA TCAAATACGT GTGGAAAATC 2251 CAAATTCTTT AATTTCTCAG AGCTTCATGT TTTTAAAAAA ATGGGTTACC 2301 AGAGACGGAA AAAATGCCAC AACTGATGCC TTAACTTCGG TCTTGACAAA 2351 AATTAATCGA ATAGATATAG TGACACTGCT AGAAGGACCA ATATTTGATT 2401 ATGGAAATAT TTCAGGCACC AGAAGTTTTG CAGATGAGAA CAATGTTTTC 2451 CATGACCCTG TTGATGGTTA TCCTTCCCTT CAAGTGGAAC TGGAAACCCC 2501 CACAGGGTTG CACTACACAC CACCTACCCC TTTCCAGCAA GATGATTATT 2551 TTAGTGATAT CTCTAGCATA GAATCTCCCC TTAGAACCCC TAGTAGACTG 2601 AGTGATGGGC TAGTGCCTTC CCAGGGGAAC ATAGAGCATT CCGCAGATGG 2651 ACCTCCAGTC GTAACTGCAG AAGACGCTTC CTTAGAAGAC AGCAAACTGG 2701 AAGACTCAGT GCCTTTAACA GAAATGCCTG AAGCAGTGAT GTAGATGAGA 2751 GCCAGTTGGA GAATGTATGT CTGAGTTGGC AGAATGAGAC ATCAAGTGGA 2801 AACCTAGAGT CCTGCGCTCA AGCTCGAAGA GTAACTGGTG GGTTACTAGA 2851 TCGACTGGAT GACAGCCCTG ACCAGTGTAG AGATTCCATT ACCTCATATC 2901 TCAAAGGAGA AGCTGGCAAA TTTGAAGCAA ATGGAAGCCA TACAGAAATC 2951 ACTCCAGAAG CAAAGACAAA ATCTTACTTT CCAGAATCCC AAAATGATGT 3001 AGGAAAACAG AGTACCAAGG AAACTCTGAA ACCAAAAATA CATGGATCTG 3051 GTCATGTTGA AGAACCAGCA TCACCACTAG CAGCATATCA GAAATCTCTA 3101 GAAGAAACCA GCAAGCTTAT AATAGAAGAG ACTAAACCCT GTGTGCCTGT 3151 CAGTATGAAA AAGATGAGTA GGACTTCTCC AGCAGATGGC AAGCCAAGGC 3201 TTAGCCTCCA TGAAGAAGAG GGGTCCAGTG GGTCTGAGCA AAAGCAGGGA 3251 GAAGGTTTTA AGGTGAAAAC GAAGAAAGAA ATCCGGCATG TGGAAAAGAA 3301 GAGCCACTCG TAACAGCGAA CGGTCAGTCA AGGATCATAA GTTTTTACTG 3351 CCAGTATTGA GAAATTCGTG GAAGAAATGT CAGCAGGAAG TAAAAATTCA 3401 CCGAGAAGTG TGTGTGTGTT CGCTGCTTCC ACACATTAAT GGCATGATTT 3451 TTTTTATGCA AAAAAAAAAA BLAST Results Entry MMANK3A_1 from database TREMBL: Ank3"; product: "ankyrin 3"; Mus mu. +3 4022 0.0 Entry HS13616 from database EMBL: Human ankyrin G (ANK-3) mRNA, complete eds. Length = 14,770 Plus Strand HSPs: Score = 8505 (1276.1 bits), Expect = 0.0, Sum P 3) = 0.0 Identities = 1799/1873 (96%) Medline entries 95394457: Chromosomal localization of the ankyπnG gene (ANK3/Ank3) to human 10q21 and mouse 10. 95138209: A new ankyrin gene with neural-specific isoforms localized at the axonal initial segment and node of Ranvier Peptide information for frame 3 ORF from 309 bp to 2741 bp; peptide length: 811 Category: known protein Classification: unset 1 MALPQSEDAM TGDTDKYLGP QDLKELGDDS LPAEGYMGFS LGARSASLRS 51 FSSDGSYTLN RSSYARDSMM IEELLVPSKE QHLTFTREFD SDSLRHYSWA 101 ADTLDNVNLV PSPIHSGFLV SFMVDARGGS MRGSRHHGMR IIIPPRKCTA 151 PTRITCRLVK RHKLANPPPM VEGEGLASRL VEMGPAGAQF LGPVIVEIPH 201 FGSMRGKERE LIVLRSENGE TWKEHQFDSK NEDLTELLNG MDEELDSPEE 251 LGKKRICRII TKDFPQYFAV VSRIKQESNQ IGPEGGILSS TTVPLVQASF 301 PEGALTKRIR VGLQAQPVPD EIVKKILGNK ATFSPIVTVE PRRRKFHKPI 351 TMTIPVPPPS GEGVSNGYKG DTTPNLRLLC SITGGTSPAQ WEDITGTTPL 401 TFIKDCVSFT TNVSARFWLA DCHQVLETVG LATQLYRELI CVPYMAKFVV 451 FAKMNDPVES SLRCFCMTDD KVDKTLEQQE NFEEVARSKD IEVLEGKPIY 501 VDCYGNLAPL TKGGQQLVFN FYSFKENRLP FSIKIRDTSQ EPCGRLSFLK 551 EPKTTKGLPQ TAVCNLNITL PAHKKIEKTD GRQSFASLAL RKRYSYLTEP 601 GMSPQSPCER TDIRMAIVAD HLGLSWTELA RELNFSVDEI NQIRVENPNS 651 LISQSFMFLK KWVTRDGKNA TTDALTSVLT KINRIDIVTL LEGPIFDYGN 701 ISGTRΞFADE NNVFHDPVDG YPSLQVELET PTGLHYTPPT PFQQDDYFSD 751 ISSIESPLRT PSRLSDGLVP SQGNIEHSAD GPPVVTAEDA SLEDSKLEDS 801 VPLTEMPEAV M BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfkd2_24p5, frame 3 TREMBL:MMANK3A_1 gene: "Ank3"; product: "ankyrin 3"; Mus musculus epithelial ankyrin 3 (Ank3) 5kb isoform mRNA, complete eds., N = 1, Score = 4022, P = 0 TREMBL :MMANK3B_3 gene: "Ank3"; product: "ankyrin 3"; Mus musculus epithelial ankyrin 3 (7kb isoform) mRNA, complete eds., N = 1, Score = 4005, P = 0 TREMBL :MMANK3B_4 gene: "Ank3"; product: "ankyrin 3"; Mus musculus epithelial ankyrin 3 (7kb isoform) mRNA, complete eds., N = 1, Score = 4005, P = 0 >TREMBL : MMANK3A_1 gene: "Ank3"; product: "ankyrin 3"; Mus musculus epithelial ankyrin 3 (Ank3) 5kb isoform mRNA, complete eds. Length = 1,094 HSPs: Score = 4022 (603.5 bits), Expect = O.Oe+00, P = O.Oe+00 Identities = 769/805 (95%), Positives = 783/805 (97%) Query: 1 MALPQSEDAMTGDTDKYLGPQDLKELGDDSLPAEGYMGFSLGARSASLRSFSSDGSYTLN 60 MALP SEDA+TGDTDKYLGPQDLKELGDDSLPAEGY+GFSLGARSASLRSFSSD SYTLN Sbjct: 1 MALPHSEDAITGDTDKYLGPQDLKELGDDSLPAEGYVGFSLGARSASLRΞFSSDRSYTLN 60 Query: 61 RSSYARDSMMIEELLVPSKEQHLTFTREFDSDSLRHYSWAADTLDNVNLVPSPIHSGFLV 120 RSSYARDSMMIEELLVPSKEQHLTFTREFDSDSLRHYSWAADTLDNVNLV SP+HSGFLV Sbjct: 61 RSSYARDSMMIEELLVPSKEQHLTFTREFDSDSLRHYSWAADTLDNVNLVΞSPVHSGFLV 120 Query: 121 SFMVDARGGSMRGSRHHGMRIIIPPRKCTAPTRITCRLVKRHKLANPPPMVEGEGLASRL 180 SFMVDARGGSMRGSRHHGMRIIIPPRKCTAPTRITCRLVKRHKLANPPPMVEGEGLASRL Sbjct: 121 SFMVDARGGΞMRGSRHHGMRIIIPPRKCTAPTRITCRLVKRHKLANPPPMVEGEGLASRL 180 Query: 181 VEMGPAGAQFLGPVIVEIPHFGSMRGKERELIVLRSENGETWKEHQFDSKNEDLTELLNG 240 VEMGPAGAQFLGPVIVEIPHFGSMRGKERELIVLRSENGETWKEHQFDSKNEDL ELLNG Sbjct: 181 VEMGPAGAQFLGPVIVEIPHFGSMRGKERELIVLRSENGETWKEHQFDSKNEDLAELLNG 240 Query: 241 MDEELDSPEELGKKRICRIITKDFPQYFAVVSRIKQESNQIGPEGGILSSTTVPLVQASF 300 MDEELDSPEELG KRICRIITKDFPQYFAVVSRIKQESNQIGPEGGILSSTTVPLVQASF Sbjct: 241 MDEELDSPEELGTKRICRIITKDFPQYFAVVSRIKQESNQIGPEGGILSSTTVPLVQASF 300 Query: 301 PEGALTKRIRVGLQAQPVPDEIVKKILGNKATFSPIVTVEPRRRKFHKPITMTIPVPPPS 360 PEGALTKRIRVGLQAQPVP+E VKKILGNKATFSPIVTVEPRRRKFHKPITMTIPVPPPS Sbjct: 301 PEGALTKRIRVGLQAQPVPEETVKKILGNKATFSPIVTVEPRRRKFHKPITMTIPVPPPS 360 Query: 361 GEGVSNGYKGDTTPNLRLLCSITGGTSPAQWEDITGTTPLTFIKDCVSFTTNVSARFWLA 420 GEGVSNGYKGD TPNLRLLCSITGGTSPAQWEDITGTTPLTFIKDCVSFTTNVSARFWLA Sbjct: 361 GEGVΞNGYKGDATPNLRLLCSITGGTSPAQWEDITGTTPLTFIKDCVSFTTNVSARFWLA 420 Query: 421 DCHQVLETVGLATQLYRELICVPYMAKFVVFAKMNDPVESSLRCFCMTDDKVDKTLEQQE 480 DCHQVLETVGLA+QLYRELICVPYMAKFVVFAK NDPVESSLRCFCMTDD+VDKTLEQQE Sbjct: 421 DCHQVLETVGLASQLYRELICVPYMAKFVVFAKTNDPVESSLRCFCMTDDRVDKTLEQQE 480 Query: 481 NFEEVARSKDIEVLEGKPIYVDCYGNLAPLTKGGQQLVFNFYSFKENRLPFSIKIRDTSQ 540 NFEEVARSKDIEVLEGKPIYVDCYGNLAPLTKGGQQLVFNFYSFKENRLPFSIKIRDTSQ Sbjct: 481 NFEEVARSKDIEVLEGKPIYVDCYGNLAPLTKGGQQLVFNFYSFKENRLPFSIKIRDTSQ 540 Query: 541 EPCGRLΞFLKEPKTTKGLPQTAVCNLNITLPAHKKIEKTDGRQSFASLALRKRYSYLTEP 600 EPCGRLSFLKEPKTTKGLPQTAVCNLNITLPAHKK EK D RQSFASLALRKRYSYLTEP Sbjct: 541 EPCGRLSFLKEPKTTKGLPQTAVCNLNITLPAHKKAEKADRRQSFASLALRKRYSYLTEP 600 Query: 601 GMSPQSPCERTDIRMAIVADHLGLSWTELARELNFSVDEINQIRVENPNSLISQSFMFLK 660 MSPQSPCERTDIRMAIVADHLGLSWTELARELNFSVDEINQIRVENPNSLISQSFM LK Sbjct: 601 SMSPQSPCERTDIRMAIVADHLGLSWTELARELNFSVDEINQIRVENPNSLISQSFMLLK 660 Query: 661 KWVTRDGKNATTDALTSVLTKINRIDIVTLLEGPIFDYGNISGTRSFADENNVFHDPVDG 720 KWVTRDGKNATTDALTSVLTKINRIDIVTLLEGPIFDYGNISGTRSFADENNVFHDPVDG Sbjct: 661 KWVTRDGKNATTDALTSVLTKINRIDIVTLLEGPIFDYGNISGTRSFADENNVFHDPVDG 720 Query: 721 YPSLQVELETPTGLHYTPPTPFQQDDYFSDISSIESPLRTPSRLSDGLVPSQGNIEHSAD 780 +PS QVELETP GL++TPP PFQQDD+FSDISSIESP RTPSRLSDGLVPSQGNIEH Sbjct: 721 HPSFQVELETPMGLYWTPPNPFQQDDHFSDISSIESPFRTPSRLSDGLVPSQGNIEHPTG 780 Query: 781 GPPVVTAEDASLEDSKLEDSVPLTE 805 GPPVVTAED SLEDSK++DSV +T+ Sbjct: 781 GPPWTAEDTSLEDSKMDDSVTVTD 805 Pedant information for DKFZphfkd2_24p5, frame 3 Report for DKFZphfkd2_24p5.3 [LENGTH] 811 [MW] 90104.66 [pi] 5.40 [HOMOL] TREMBL :MMANK3A_1 gene: "Ank3"; product: "ankyrin 3"; Mus musculus epithelial ankyrin 3 (Ank3) 5kb isoform mRNA, complete eds. 0.0 [BLOCKS] BL50017B Death domain proteins profile [PIRKW] phosphoprotein 0.0 [PIRKW] alternative splicing 0.0 [PIRKW] peripheral membrane protein 0.0 [PIRKW] cytoskeleton 0.0 [SUPFAM] ankyrin 0.0 [SUPFAM] ankyrin repeat homology 0.0 [SUPFAM] unassigned ankyrin repeat proteins 0.0 [KW] TRANSMEMBRANE 2 [KW] LOW COMPLEXITY 1.73 % SEQ MALPQSEDAMTGDTDKYLGPQDLKELGDDSLPAEGYMGFSLGARSASLRSFSSDGSYTLN SEG PRD cccccccccccccccccccccccccccccccccccccccccccccceeeeeccccccccc MEM SEQ RSSYARDSMMIEELLVPSKEQHLTFTREFDSDSLRHYSWAADTLDNVNLVPSPIHSGFLV SEG PRD cccchhhhhhhhheeeehhhhhhhhhhhccccccccccccccccccccccccccccceee MEM MMMMMMMMMMMM SEQ ΞFMVDARGGSMRGSRHHGMRIIIPPRKCTAPTRITCRLVKRHKLANPPPMVEGEGLASRL SEG xxxxxxxxxxxxxx PRD eeeeeccccccccccccceeeecccccccccceeeeehhhhhccccccccccccccccee MEM MMMMMMMMMMMMMMMM M SEQ VEMGPAGAQFLGPVIVEIPHFGSMRGKERELIVLRSENGETWKEHQFDSKNEDLTELLNG SEG PRD eecccccceeeceeeeeeccccccccccceeeeeeccccceeeeeccccccchhhhhhhc MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMM SEQ MDEELDSPEELGKKRICRIITKDFPQYFAVVSRIKQESNQIGPEGGILSSTTVPLVQASF SEG PRD cccccchhhhhhhhheeeeeeccccceeeeehhhhhcccccccccccccceeeeeeeccc MEM SEQ PEGALTKRIRVGLQAQPVPDEIVKKILGNKATFSPIVTVEPRRRKFHKPITMTIPVPPPS SEG PRD ccchhhhhhhhhhhhhccccceeeeccccccccccceeeccccccccccceeeecccccc MEM SEQ GEGVSNGYKGDTTPNLRLLCSITGGTSPAQWEDITGTTPLTFIKDCVSFTTNVSARFWLA SEG PRD ccccccccccccccceeeeeeeeccccccccccccccceeeeeeccccccccccceeeec MEM SEQ DCHQVLETVGLATQLYRELICVPYMAKFVVFAKMNDPVESSLRCFCMTDDKVDKTLEQQE SEG PRD cchhhhhhhhhhhhhhhhhhhhcchhhhhheeecccchhhhhhhhccccchhhhhhhhhc MEM SEQ NFEEVARSKDIEVLEGKPIYVDCYGNLAPLTKGGQQLVFNFYSFKENRLPFSIKIRDTSQ SEG PRD cceeecccceeeeeeccceeeeecccccccchhhhhhhhhchhhhhhhcceeeeeecccc MEM SEQ EPCGRLSFLKEPKTTKGLPQTAVCNLNITLPAHKKIEKTDGRQSFASLALRKRYSYLTEP SEG ' PRD ccccceeeeccccccccccccccccccccccccccccccccchhhhhhhhhhhhheeecc MEM SEQ GMSPQΞPCERTDIRMAIVADHLGLSWTELARELNFSVDEINQIRVENPNSLISQSFMFLK SEG PRD ccccccchhhhhhhhhhhhhhhccchhhhhhhhhhhhhhcceeeeecccchhhhhhhhhh MEM SEQ KWVTRDGKNATTDALTSVLTKINRIDIVTLLEGPIFDYGNISGTRSFADENNVFHDPVDG SEG PRD hhhhcccccccchhhhhhhhhhcceeeeeeeccccccccccccccccccccccccccccc MEM SEQ YPSLQVELETPTGLHYTPPTPFQQDDYFSDISΞIESPLRTPSRLSDGLVPSQGNIEHSAD SEG PRD cccceeeeeccccccccccccccccccccceeeccccccccccccccccccccccccccc MEM SEQ GPPVVTAEDASLEDSKLEDSVPLTEMPEAVM SEG PRD ccceeeecccccccccccccccccccccccc MEM (No Prosite data available for DKFZphfkd2_24p5.3) (No Pfam data available for DKFZphfkd2_24p5.3) DKFZphfkd2_3ιl3 group: transmembrane protein DKFZphfkd2_3ιl3 encodes a novel 406 ammo acid protein with C. elegans cosmid Y37D8A and A. thaliana H71412 hypothetical protein. The novel protein contains 3 transmembrane regions. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of kidney-specific genes and as a new marker for kidney cells. similarity to A. thaliana and C. elegans; membrane regions : 3 complete cDNA, complete eds, EST hits Sequenced by BMFZ Locus: /map="17" Insert length: 2052 bp Poly A stretch at pos. 2032, no polyadenylation signal found 1 AGTGACGTGA GCGGGTTCCG GTTGTCTGGA GCCCAGCGGC GGGTGTGAGA 51 GTCCGTAAGG AGCAGCTTCC AGGATCCTGA GATCCGGAGC AGCCGGGGTC 101 GGAGCGGCTC CTCAAGAGTT ACTGATCTAT GAAATGGCAG AGAATGGAAA 151 AAATTGTGAC CAGAGACGTG TAGCAATGAA CAAGGAACAT CATAATGGAA 201 ATTTCACAGA CCCCTCTTCA GTGAATGAAA AGAAGAGGAG GGAGCGGGAA 251 GAAAGGCAGA ATATTGTCCT GTGGAGACAG CCGCTCATTA CCTTGCAGTA 301 TTTTTCTCTG GAAATCCTTG TAATCTTGAA GGAATGGACC TCAAAATTAT 351 GGCATCGTCA AAGCATTGTG GTGTCTTTTT TACTGCTGCT TGCTGTGCTT 401 ATAGCTACGT ATTATGTTGA AGGAGTGCAT CAACAGTATG TGCAACGTAT 451 AGAGAAACAG TTTCTTTTGT ATGCCTACTG GATAGGCTTA GGAATTTTGT 501 CTTCTGTTGG GCTTGGAACA GGGCTGCACA CCTTTCTGCT TTATCTGGGT 551 CCACATATAG CCTCAGTTAC ATTAGCTGCT TATGAATGCA ATTCAGTTAA 601 TTTTCCCGAA CCACCCTATC CTGATCAGAT TATTTGTCCA GATGAAGAGG 651 GCACTGAAGG AACCATTTTT TTGTGGAGTA TCATCTCAAA AGTTAGGATT 701 GAAGCCTGCA TGTGGGGTAT CGGTACAGCA ATCGGAGAGC TGCCTCCATA 751 TTTCATGGCC AGAGCAGCTC GCCTCTCAGG TGCTGAACCA GATGATGAAG 801 AGTATCAGGA ATTTGAAGAG ATGCTGGAAC ATGCAGAGTC TGCACAAGAC 851 TTTGCCTCCC GGGCCAAACT GGCAGTTCAA AAACTAGTAC AGAAAGTTGG 901 ATTTTTTGGA ATTTTGGCCT GTGCTTCAAT TCCAAATCCT TTATTTGATC 951 TGGCTGGAAT AACGTGTGGA CACTTTCTGG TACCTTTTTG GACCTTCTTT 1001 GGTGCAACCC TAATTGGAAA AGCAATAATA AAAATGCATA TCCAGAAAAT 1051 TTTTGTTATA ATAACATTCA GCAAGCACAT AGTGGAGCAA ATGGTGGCTT 1101 TCATTGGTGC TGTCCCCGGC ATAGGTCCAT CTCTGCAGAA GCCATTTCAG 1151 GAGTACCTGG AGGCTCAACG GCAGAAGCTT CACCACAAAA GCGAAATGGG 1201 CACACCACAG GGAGAAAACT GGTTGTCCTG GATGTTTGAA AAGTTGGTCG 1251 TTGTCATGGT GTGTTACTTC ATCCTATCTA TCATTAACTC CATGGCACAA 1301 AGTTATGCCA AACGAATCCA GCAGCGGTTG AACTCAGAGG AGAAAACTAA 1351 ATAAGTAGAG AAAGTTTTAA ACTGCAGAAA TTGGAGTGGA TGGGTTCTGC 1401 CTTAAATTGG GAGGACTCCA AGCCGGGAAG GAAAATTCCC TTTTCCAACC 1451 TGTATCAATT TTTACAACTT TTTTCCTGAA AGCAGTTTAG TCCATACTTT 1501 GCACTGACAT ACTTTTTCCT TCTGTGCTAA GGTAAGGTAT CCACCCTCGA 1551 TGCAATCCAC CTTGTGTTTT CTTAGGGTGG AATGTGATGT TCAGCAGCAA 1601 ACTTGCAACA GACTGGCCTT CTGTTTGTTA CTTTCAAAAG GCCCACATGA 1651 TACAATTAGA GAATTCCCAC CGCACAAAAA AAGTTCCTAA GTATGTTAAA 1701 TATGTCAAGC TTTTTAGGCT TGTCACAAAT GATTGCTTTG TTTTCCTAAG 1751 TCATCAAAAT GTATATAAAT TATCTAGATT GGATAACAGT CTTGCATGTT 1801 TATCATGTTA CAATTTAATA TTCCATCCTG CCCAACCCTT CCTCTCCCAT 1851 CCTCAAAAAA GGGCCATTTT ATGATGCATT GCACACCCTC TGGGGAAATT 1901 GATCTTTAAA TTTTGAGACA GTATAAGGAA AATCTGGTTG GTGTCTTACA 1951 AGTGAGCTGA CACCATTTTT TATTCTGTGT ATTTAGGATG AAGTCTTGAA 2001 AAAAACTTTA TAAAGACATC TTTAATCATT CCAAAAAAAA AAAAAAAAAA 2051 AA BLAST Results Entry AC004686 from database EMBL: *** SEQUENCING IN PROGRESS *** Homo sapiens chromosome 17, clone hRPC.1073_F_15; HTGS phase 1, 8 unordered pieces. Score = 4142, P = 6.1e-199, identities = 830/832 Medline entries " ^ No Medline entry Peptide information for frame 2 ORF from 134 bp to 1351 bp; peptide length: 406 Category: similarity to unknown protein 1 MAENGKNCDQ RRVAMNKEHH NGNFTDPSSV NEKKRREREE RQNIVLWRQP 51 LITLQYFSLE ILVILKEWTS KLWHRQSIVV SFLLLLAVLI ATYYVEGVHQ 101 QYVQRIEKQF LLYAYWIGLG ILSSVGLGTG LHTFLLYLGP HIASVTLAAY 151 ECNSVNFPEP PYPDQIICPD EEGTEGTIFL WSIISKVRIE ACMWGIGTAI 201 GELPPYFMAR AARLSGAEPD DEEYQEFEEM LEHAESAQDF ASRAKLAVQK 251 LVQKVGFFGI LACASIPNPL FDLAGITCGH FLVPFWTFFG ATLIGKAIIK 301 MHIQKIFVII TFSKHIVEQM VAFIGAVPGI GPSLQKPFQE YLEAQRQKLH 351 HKSEMGTPQG ENWLSWMFEK LVVVMVCYFI LSIINSMAQS YAKRIQQRLN 401 SEEKTK BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfkd2_3ιl3, frame 2 TREMBL :CEY37D8A_20 gene: "Y37D8A.22"; Caenorhabditis elegans cosmid Y37D8A, N = 1, Score = 905, P = 8.8e-91 TREMBL:ATAC98_2 gene: "YUP8H12.2"; Arabidopsis thaliana chromosome 1 YAC yUP8H12 complete sequence., N = 1, Score = 470, P = l.le-44 PIR:H71412 hypothetical protein - Arabidopsis thaliana, N = 1, Score = 293, P = 6e-24 >TREMBL:CEY37D8A_20 gene: "Y37D8A.22"; Caenorhabditis elegans cosmid Y37D8A Length = 457 HSPs: Score = 905 (135.8 bits), Expect = 8.8e-91, P = 8.8e-91 Identities = 167/317 (52%), Positives = 228/317 (71%) Query: 38 REERQNIVLWRQPLITLQYFSLEILVILKEWTSKLWHRQSIVVSFLLLLAVLIATYYVEG 97 R ER+ IV WR+P I + Y +EI + E K+ +++++ + + + + Y+ G Sbjct: 93 RMERETIVFWRRPHIVIPYALMEIAHLAVELFFKILAHKTVLLLTAISIGLAVYGYHAPG 152 Query: 98 VHQQYVQRIEKQFLLYAYWIGLGILSSVGLGTGLHTFLLYLGPHIASVTLAAYECNSVNF 157 HQ++VQ IEK L +++W+ LG+LSS+GLG+GLHTFL+YLGPHIA+VT+AAYEC S++F Sbjct: 153 AHQEHVQTIEKHILWWSWWVLLGVLSSIGLGSGLHTFLIYLGPHIAAVTMAAYECQSLDF 212 Query: 158 PEPPYPDQIICPDEEGTEGTIFLWSIISKVRIEACMWGIGTAIGELPPYFMARAARLSGA 217 P+PPYP+ I CP + + F W I++KVR+E+ +WG GTA+GELPPYFMARAAR+SG Sbjct: 213 PQPPYPESIQCPSTKSSIAVTF-WQIVAKVRVESLLWGAGTALGELPPYFMARAARIΞGQ 271 Query: 218 EPDDEEYQEFEEMLE-HAESAQD FASRAKLAVQKLVQKVGFFGILACASIPNPLFD 272 EPDDEEY+EF E++ ES D RAK V+ + ++GF GIL ASIPNPLFD Sbjct: 272 EPDDEEYREFLELMNADKESDADQKLSIVERAKSWVEHNIHRLGFPGILLFASIPNPLFD 331 Query: 273 LAGITCGHFLVPFWTFFGATLIGKAIIKMHIQKIFVIITFSKHIVEQMVAFIGAVPGIGP 332 LAGITCGHFLVPFW+FFGATLIGKA++KMH+Q FVI+ FS H E V + +P +GP Sbjct: 332 LAGITCGHFLVPFWSFFGATLIGKALVKMHVQMGFVILAFSDHHAENFVKILEKIPAVGP 391 Query: 333 SLQKPFQEYLEAQRQKLH 350 +++P + LE QR+ LH Sbjct: 392 YIRQPISDLLEKQRKALH 409 Pedant information for DKFZphfkd2_3ιl3, frame 2 Report for DKFZphf d2_3ιl3.2 [LENGTH] 406 [MW] 46298.17 [pi] 6.47 [HOMOL] TREMBL :CEY37D8A_20 gene: "Y37D8A.22"; Caenorhabditis elegans cosmid Y37D8A le- 79 [PROSITE] MYRISTYL 10 [PROSITE] CK2_PHOSPHO_SITE 3 [PROSITE] PKC_PHOSPHO_SITE 1 [PROSITE] ASNJΞLYCOSYLATION 1 [KW] TRANSMEMBRANE 3 [KW] LOW COMPLEXITY 9.85 % SEQ MAENGKNCDQRRVAMNKEHHNGNFTDPSSVNEKKRREREERQNIVLWRQPLITLQYFSLE SEG xxxxxxxxxx PRD ccccccchhhhhhhhhhhccccccccccccchhhhhhhhhhhhhhhhccccchhhhhhhh MEM MMMMMMMMMMMMMMMMMMMMMMMMMMM SEQ ILVILKEWTSKLWHRQSIVVSFLLLLAVLIATYYVEGVHQQYVQRIEKQFLLYAYWIGLG SEG xxxx PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhheeeccchhhhhhhhhhhhhhhhhhhhhh MEM MM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM SEQ ILSSVGLGTGLHTFLLYLGPHIASVTLAAYECNSVNFPEPPYPDQIICPDEEGTEGTIFL SEG xxxxxxxxxxx PRD hccccccccceeeeeeeccchhhhhhhhhhhccccccccccccccccccccccccceeee MEM SEQ WSIISKVRIEACMWGIGTAIGELPPYFMARAARLSGAEPDDEEYQEFEEMLEHAESAQDF SEG xxxxxxxxxxxxxxx PRD eehhhhhhhhhhhhhccccccccccchhhhhhhhcccccchhhhhhhhhhhhhhhhhhhh MEM SEQ ASRAKLAVQKLVQKVGFFGILACASIPNPLFDLAGITCGHFLVPFWTFFGATLIGKAIIK SEG PRD hhhhhhhhhhhhhhhcceeeeeeeecccccccccccccccceeeeeeehhhhhhhhhhhh MEM MMMMMMMMMMMMMMMMMMMMM SEQ MHIQKIFVIITFSKHIVEQMVAFIGAVPGIGPSLQKPFQEYLEAQRQKLHHKSEMGTPQG SEG PRD hhhhheeeeeeechhhhhhhhhhhhccccccccchhhhhhhhhhhhhhhhhhcccccccc MEM SEQ ENWLSWMFEKLVVVMVCYFILSIINSMAQSYAKRIQQRLNSEEKTK SEG PRD cchhhhhhhhhheeehhhhhhhhhhhhhhhhhhhhhhhhhhhcccc MEM Prosite for DKFZphfkd2_3ιl3.2 PS00001 23->27 ASN_GLYCOSYLATION PDOC00001 PS00005 69->72 PKC_PHOSPHO_SITE PDOC00005 PS00006 29->33 CK2_PHOSPHO_SITE PDOC00006 PS00006 215->219 CK2_PHOSPHO_SITE PDOC00006 PS00006 236->240 CK2_PHOSPHO_SITE PDOC00006 PS00008 120->126 MYRISTYL PDOC00008 PS00008 126-M32 MYRISTYL PDOC00008 PS00008 173->179 MYRISTYL PDOC00008 PS00008 195->201 MYRISTYL PDOC00008 PS00008 197->203 MYRISTYL PDOC00008 PS00008 259->265 MYRISTYL PDOC00008 PS00008 275->281 MYRISTYL PDOC00008 PS00008 325->331 MYRISTYL PDOC00008 PS00008 329->335 MYRISTYL PDOC00008 PS00008 356->362 MYRISTYL PDOC00008 (No Pfam data available for DKFZphfkd2_3ιl3.2) DKFZphfkd2_3ol7 group: metabolism DKFZphfkd2_3ol7 encodes a novel 72 amino acid protein with similarity to bos taurus NADH- ubiquinone oxidoreductase B33 subunit (EC 1.6.5.3) (EC 1.6.99.3). NADH:ubιqumone oxidoreductase is the first enzyme in the respiratory electron transport chain of mitochondria. It is a a membrane-bound multi-subunit protein. The bovine heart enzyme contains about 40 different polypeptides. The novel protein is the human orthologue of bovme B22. The new protein can find application in modulation of the respiratory electron transport chain pathways of mitochondria. strong similarity to bovme NADH-UBIQUINONE OXIDOREDUCTASE B22 subunit complete cDNA, complete eds, EST hits, in frame stop codon at -274 will be checked ESTs HS1291620/AA883920 show no stop codon at this side Sequenced by BMFZ Locus: unknown Insert length: 693 bp Poly A stretch at pos. 670, polyadenylation signal at pos. 659 1 CAGCAGGCGT GCAGTTTCCC GGCTCTCCGC GCGGCCGGGG AAGGTCAGCG 51 CCGTAATGGC GTTCTTGGCG TCGGGACCCT ACCTGACCCA TCAGCAAAAG 101 GTGTTGCGGC TTTATAAGCG GGCGCTACGC CACCTCGAGT CGTGGTGCGT 151 CCAGAGAGAC AAATACCGAT ACTTTGCTTG TTTGATGAGA GCCCGGTTTG 201 AAGAACATAA GAATGAAAAG GATATGGCGA AGGCCACCCA GCTGCTGAAG 251 GAGGCCGAGG AAGAATTCTG GTAACGTCAG CATCCACAGC CATACATCTT 301 CCCTGACTCT CCTGGGGGCA CCTCCTATGA GAGATACGAT TGCTACAAGG 351 TCCCAGAATG GTGCTTAGAT GACTGGCATC CTTCTGAGAA GGCAATGTAT 401 CCTGATTACT TTGCCAAGAG AGAACAGTGG AAGAAACTGC GGAGGGAAAG 451 CTGGGAACGA GAGGTTAAGC AGCTGCAGGA GGAAACGCCA CCTGGTGGTC 501 CTTTAACTGA AGCTTTGCCC CCTGCCCGAA AGGAAGGTGA TTTGCCCCCA 551 CTGTGGTGGT ATATTGTGAC CAGACCCCGG GAGCGGCCCA TGTAGAAAGA 601 GAGAGACCTC ATCTTTCATG CTTGCAAGTG AAATATGTTA CAGAACATGC 651 ACTTGCCCTA ATAAAAAATC AGTAAAAAAA AAAAAAAAAA AAA BLAST Results Entry S28256 from database PIR: NADH dehydrogenase (ubiquinone) (EC 1.6.5.3) chain CI-B22 - bovine >TREMBL:MIBTCIB22_1 gene: "CI-B22"; product: "NADH-ubiquinone oxidoreductase complex B22 subunit"; B. taurus mitochondrion cI-B22 mRNA for B22 subunit of the NADH-ubiqumone oxidoreductase complex Score = 933, P = 5.2e-93, identities = 163/179, positives = 172/179, frame +2 Medline entries 92389317 Sequences of 20 subunits of NADH: ubiquinone oxidoreductase from RT bovme heart mitochondria. Application of a novel strategy for RT sequencing proteins using the polymerase chain reaction Peptide information for frame 2 ORF from 56 bp to 271 bp; peptide length: 72 Category: strong similarity to known protein 1 MAFLASGPYL THQQKVLRLY KRALRHLESW CVQRDKYRYF ACLMRARFEE 51 HKNEKDMAKA TQLLKEAEEE FW*RQHPQPY IFPDSPGGTS YERYDCYKVP 101 EWCLDDWHPS EKAMYPDYFA KREQWKKLRR ESWEREVKQL QEETPPGGPL 151 TEALPPARKE GDLPPLWWYI VTRPRERPM BLASTP hits Sequences producing significant alignments: (bits) Value sp|Q02369|NI2M_BOVIN|0D36CE17281FB735 (NDUFB9.. ) NADH-UBIQUINONE... 141 7e-34 tr|U41534 | Q18036 I D34BCCB6E8FBCD5F (C16A3.4) SIMILAR TO NADH-UBIQ... 53 3e-07 >sp|Q02369|NI2M_BOVIN|0D36CE17281FB735 (NDUFB9.. ) NADH-UBIQUINONE OXIDOREDUCTASE B22 SUBUNIT (EC 1.6.5.3) (EC 1.6.99.3) (COMPLEX I-B22) (CI-B22) . [BOS TAURUS] Length = 178 Score = 141 bits (351), Expect = 7e-34 Identities = 63/71 (88%), Positives = 68/71 (95%) Query: 2 AFLASGPYLTHQQKVLRLYKRALRHLESWCVQRDKYRYFACLMRARFEEHKNEKDMAKAT 61 AFL+SG YLTHQQKVLRLYKRALRHLESWC+ RDKYRYFACL+RARF+EHKNEKDM KAT Sbjct: 1 AFLSSGAYLTHQQKVLRLYKRALRHLESWCIHRDKYRYFACLLRARFDEHKNEKDMVKAT 60 Query: 62 QLLKEAEEEFW 72 QLL+EAEEEFW Sbjct: 61 QLLREAEEEFW 71 >tr|U41534 | Q18036 | D34BCCB6E8FBCD5F (C16A3.4) SIMILAR TO NADH-UBIQUINONE OXIDOREDUCTASE B22. [CAENORHABDITIS ELEGANS] Length = 163 Score = 52.7 bits (124), Expect = 3e-07 Identities = 25/64 (39%), Positives = 41/64 (64%), Gaps = 1/64 (1%) Query 10 LTHQQKVLRLYKRALRHLESWCVQRD-KYRYFACLMRARFEEHKNEKDMAKATQLLKEAE 68 L+H+QKV RLYKR LR +++W + + R+ C++RARF+ + +E D K+ LL + Sbjct 12 LSHRQKVTRLYKRCLREVDNWYGGNNLEVRFQKCIIRARFDANADEVDTRKSQILLADGC 71 Query 69 EEFW 72 + W Sbjct 72 RQLW 75 Alert BLASTP hits for DKFZphfkd2_3ol7, frame 2 No Alert BLASTP hits found Pedant information for DKFZphfkd2_3ol7, frame 2 Report for DKFZphfkd2_3ol7.2 [LENGTH] 72 [MW] 8839.28 [pi] 9.26 [HOMOL] PIR:S28256 NADH dehydrogenase (ubiquinone) (EC 1.6.5.3) chain CI-B22 - bovme 2e-34 [KW] All_Alpha SEQ MAFLASGPYLTHQQKVLRLYKRALRHLESWCVQRDKYRYFACLMRARFEEHKNEKDMAKA PRD ccccccccchhhhhhhhhhhhhhhhhhhhhhccchhhhhhhhhhhhhhhhhcchhhhhhh SEQ TQLLKEAEEEFW PRD hhhhhhhhhccc (No Prosite data available for DKFZphfkd2_3ol7.2 ) (No Pfam data available for DKFZphfkd2_3ol7.2) DKFZphfkd2 46a6 group: kidney derived DKFZphf d2_46a6 encodes a novel 315 amino acid protein without similarity to known proteins. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of kidney-specific genes . unknown complete cDNA, complete eds, EST hits Sequenced by MediGenomix Locus: /map="228.6 cR from top of Chrl5 linkage group" Insert length: 2774 bp Poly A stretch at pos. 2751, polyadenylation signal at pos. 2732 1 CTCGCGAGCG CAGCTATGGC TGCTGGCGTA CCCTGTGCGT TAGTCACCAG 51 CTGCTCCTCC GTCTTCTCAG GAGACCAGCT GGTCCAACAT ACCCTTGGAA 101 CAGAAGATCT TATTGTGGAA GTGACTTCCA ATGATGCTGT GAGATTTTAT 151 CCCTGGACCA TTGATAATAA ATACTATTCA GCAGACATCA ATCTATGTGT 201 GGTGCCAAAC AAATTTCTTG TTACTGCAGA GATTGCAGAA TCTGTCCAAG 251 CATTTGTGGT TTACTTTGAC AGCACACGAA AATCGGGCCT TGATAGTGTC 301 TCCTCATGGC TTCCACTGGC AAAAGCATGG TTACCTGAGG TGATGATCTT 351 GGTCTGCGAT AGAGTGTCTG AAGATGGTAT AAACCGACAA AAAGCTCAAG 401 AATGGAGCCT CAAACATGGC TTTGAATTGG TAGAACTTAG TCCAGAGGAG 451 TTGCCTGAGG AGGATGATGA CTTCCCAGAA TCTACAGGAG TAAAGCGAAT 501 TGTCCAAGCC CTGAATGCCA ATGTGTGGTC CAATGTAGTG ATGAAGAATG 551 ATAGGAACCA AGGCTTTAGC CTTCTCAACT CATTGACTGG AACAAACCAT 601 AGCATTGGGT CAGCAGATCC CTGTCACCCA GAGCAACCCC ATTTGCCAGC 651 AGCAGATAGT ACTGAATCCC TCTCTGATCA TCGGGGTGGT GCATCTAACA 701 CAACAGATGC CCAGGTTGAT AGCATTGTGG ATCCCATGTT AGATCTGGAT 751 ATTCAAGAAT TAGCCAGTCT TACCACTGGA GGAGGAGATG TGGAGAATTT 801 TGAAAGACCC TTTTCAAAGT TAAAGGAAAT GAAAGACAAG GCTGCGACGC 851 TTCCTCATGA GCAAAGAAAA GTGCATGCAG AAAAGGTGGC CAAAGCATTC 901 TGGATGGCAA TCGGGGGAGA CAGAGATGAA ATTGAAGGCC TTTCATCTGA 951 TGGAGAGCAC TGAATTATTC ATACTAGGGT TTGACCAACA AAGATGCTAG 1001 CTGTCTCTGA GATACCTCTC TACTCAGCCC AGTCATATTT TGCCAAAATT 1051 GCCCTTATCA TGTTGGCTGC CTGACTTGTT TATAGGGTCC CCTTAATTTT 1101 AGTTTTTAGT AGGAGGTTAA GGAGAAATCT TTTTTTTCCT CAGTATATTG 1151 TAAGAGAGTG AGGAATACAG TGATAGTAAT GAGTGAGGAT TTCTTAAATA 1201 TACTTTTTTT TTGTTCTAGG AATGAGGGTA GGATAAATCT CAGAGGTCTG 1251 TGTGATTTAC TCAAGTTGAA GACAACCTCC AGGCCATTCC TGGTCAACCT 1301 TTTAAGTAGC ATTTCCAGCA TTCACACTTG ATACTGCACA TCAGGAGTTG 1351 TGTCACCTTT CCTGGGTGAT TTGGGTTTTC TCCATTCAAG GAGCTTGTAG 1401 CTCTGAGCTA TGATGCTTTT ATTGGGAGGA AAGGAGGCAG CTGCAGAATT 1451 GATGTGAGCT ATGTGGGGCC GAAGTCTCAG CCCGCAGCTA AGTCTCTACC 1501 TAAGAAAATG CCTCTGGGCA TTCTTTTGAA GTATAGTGTC TGAGCTCATG 1551 CTAGAAAGAA TCAAAAAGCC AGTGTGGATT TTTAGGCTGT AATAAATGAG 1601 GCAAAGGATT TCTATTCCAG TGGGAAGGAA ACCTCTCTAC TGAGTTGTGG 1651 GGGATATGTT GTATGTTAGA GAGAACCTTA AGGAGTCCTT GTATGGGCCA 1701 TGGAGACAGT ATGTGATAAC ATACCGTGAT TTTCATGAAG AAATTCTTCT 1751 GTCCTAGAGT TCTCCCCTGC TGCTTGAGAT GCCAGAGCTG TGTTGTTGCA 1801 CACCTGCAAA ACAAGGCACA TTTCCCCCTT TCTCTTTAAA GCCAAAGAGA 1851 GATCACTGCC AAAGTGGGAG CACTAAGGGG TGGGTGGGGA AGTGAAATGT 1901 TAGGCGATGA ATTCCTGAGC ACCTTGTTTT TCTTCCAAGG TTCGTAGCTC 1951 CTCTCTGCCC TTCCAAGCCT GTAACCTCGG AGGACTATCT TTTGTTCTCT 2001 ATCCTTTGTC TTGTTAGAGT GGGTCAGCCC CAGAGGAACT GATAAGCAAA 2051 TGGCAAGTTT TTAAAGGAAG AGTGGAAAGT ACTGCAAATA AAAATCCTTA 2101 TTTGTTTTTG TAGACTTTGT AATGCATATC ATTAGCCCTC ACTGTGATCA 2151 TTACTGCTGT GGCTCTGAAC TGGCACATAG TACAGTGGAT GGAAGGTGCC 2201 CGCACACCAG CTGAGAACTG GTTCTGGCCT AGGTGGGCTC TAGAACCATT 2251 TACACAGCAT GAAAGAAACA GGTTGGGTTA GGAGCAGAAA GAAATAAGGC 2301 TCACACCCCT CCAGACACTA CCTTATAAGC ACTGCAGAAC CTGAAACAGA 2351 TGGCAGAAGG AATGGAATGC TACAGGGGCC AGCAGGAGTG ACCACAGGGA 2401 GGGGACAGCT CAGTGACTGG AGCATTCAGG AAGAGGCTTT CCAGGGAACA 2451 CTGGACATTG CTTAGTGACC TTTTGTTCCT TTTTTTTTTT TTTTCTTTTA 2501 CTGTTCTGAA AGACTTTGAG TCTGTGGTTC ACCACCAGCC CATCAGTGTT 2551 TCTTTGAGGT GATTGCATTA GGGAAGTTGG CTCTGGGATT GCAAAAAAAA 2601 AAAAAAGGTG GAACATGTTT TCCTTAAAAG ATGGAAGGTT TTAGAAAATA 2651 TACTAGGCCA TCTGGTTAGA AAAAACAGAC CAGACTAGAA AAAGCTGTGA 2701 ATTTGATTTT GTAGATTAAA CAAAGCCAGA TGATTAAAAT GTGATTTATT 2751 TATAAAAAAA AAAAAAAAAA AAAA BLAST Results Entry HS463358 from database EMBL: human STS WI-14364. Length = 472 Minus Strand HSPs: Score = 1605 (240.8 bits), Expect = 5.0e-68, P = 5.0e-68 Identities = 347/361 (96%) Medline entries No Medlme entry Peptide information for frame 1 ORF from 16 bp to 960 bp; peptide length: 315 Category: putative protein Classification: unset 1 MAAGVPCALV TSCSSVFSGD QLVQHTLGTE DLIVEVTSND AVRFYPWTID 51 NKYYSADINL CVVPNKFLVT AEIAESVQAF VVYFDSTRKS GLDSVSSWLP 101 LAKAWLPEVM ILVCDRVSED GINRQKAQEW SLKHGFELVE LSPEELPEED 151 DDFPESTGVK RIVQALNANV WSNVVMKNDR NQGFSLLNSL TGTNHSIGSA 201 DPCHPEQPHL PAADSTESLS DHRGGASNTT DAQVDSIVDP MLDLDIQELA 251 SLTTGGGDVE NFERPFSKLK EMKDKAATLP HEQRKVHAEK VAKAFWMAIG 301 GDRDEIEGLS SDGEH BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfkd2_46a6, frame 1 PIR:T04362 probable GTP-bindmg protein yptm3 - maize, N = 1, Score = 87, P = 0.21 PIR:S71585 GTP-bindmg protein GB2 - Arabidopsis thaliana, N = 1, Score = 86, P = 0.27 >PIR:T04362 probable GTP-bmding protein yptm3 - maize Length = 210 HSPs: Score = 87 (13.1 bits), Expect = 2.4e-01, P = 2.1e-01 Identities = 34/160 (21%), Positives = 67/160 (41%) Query: 48 TIDNKYYSADINLCVVPNKFL-VTAEIAESVQAFVVYFDSTRKSGLDSVSSWLPLAKAWL 106 TIDNK I F +T ++ +D TR+ + ++SWL A+ Sbjct: 49 TIDNKPIKLQIWDTAGQESFRSITRSYYRGAAGALLVYDITRRETFNHLASWLEDARQHA 108 Query: 107 PE VMIL--VCDRVSEDGINRQKAQEWSLKHGFELVELSPEELPEEDDDFPESTGVKR 161 VM++ CD ++ ++ ++++ +HG +E S + ++ F ++ G Sbjct: 109 NANMTVMLIGNKCDLSHRRAVSYEEGEQFAKEHGLVFMEASAKTAQNVEEAFIKTAGT— 166 Query: 162 IVQALNANVWSNVVMKNDRNQGFSLLNSLTGTNHSIGSADPC 203 I + + ++ N G+++ NS G Ξ A C Sbjct: 167 IYKKIQDGIFDVSNESNGIKVGYAVPNSSGGGAGSSSQAGGC 208 Pedant information for DKFZphf d2_46a6, frame 1 Report for DKFZphfkd2_46a6.1 [LENGTH] 315 [MW] 34505.54 [pi] 4.55 [KW] Alpha_Beta [KW] LOW COMPLEXITY 6.67 SEQ MAAGVPCALVTSCSSVFSGDQLVQHTLGTEDLIVEVTΞNDAVRFYPWTIDNKYYSADINL SEG PRD cccccceeeeecccccccccceeeeccccceeeeeeccccceeeecccccccccccccee SEQ CVVPNKFLVTAEIAESVQAFVVYFDSTRKSGLDSVΞSWLPLAKAWLPEVMILVCDRVSED SEG PRD eeecccchhhhhhhhhhheeeeeeecccccccccccccccccccccccceeeeccccccc SEQ GINRQKAQEWSLKHGFELVELSPEELPEEDDDFPESTGVKRIVQALNANVWSNVVMKNDR SEG xxxxxxxxxxxxxxxxxxxxx PRD cchhhhhhhhhhcccceeeeccccccccccccccccccchhhhhhhhcccceeeeeeccc SEQ NQGFSLLNSLTGTNHSIGSADPCHPEQPHLPAADSTESLSDHRGGASNTTDAQVDΞIVDP SEG PRD ccccccccccccccccccccccccccccccccccccccccccccccccccccccccccch SEQ MLDLDIQELASLTTGGGDVENFERPFSKLKEMKDKAATLPHEQRKVHAEKVAKAFWMAIG SEG PRD hhhhhhhhhhhcccccccccccchhhhhhhhhhhhhhhccchhhhhhhhhhhhhhhhhhc SEQ GDRDEIEGLSSDGEH SEG PRD ccccccccccccccc (No Prosite data available for DKFZphfkd2_46a6.1) (No Pfam data available for DKFZphfkd2_46a6.1) DKFZphfkd2_46bl0 group: kidney derived DKFZphfkd2_46bl0.1 encodes a novel 315 ammo acid protein with similarity to C. elegans cosmide F25B5.3 The novel protein contains a HTH-LYSR-family PROSITE pattern. Proteins of the lysR family are bacterial transcriptional regulatory proteins which bind DNA using a helix-turn-helix motif. Most of these proteins are transcription activators and usually negatively regulate their own expression. They all possess a potential 'helix-turn-helix ' DNA-bmding motif m their N- terminal section. The ' helix-turn-helix ' motif is missing in DKFZphfkd2_46a6.1. No informative BLAST results, no predictive PFAM or SCOP motive. The new protein can find application in studying the expression profile of kidney-specific genes . similarity to C. elegans F25B5.3 complete cDNA, complete eds, EST hits Sequenced by MediGenomix Locus: unknown Insert length: 1285 bp Poly A stretch at pos. 1266, no polyadenylation signal found 1 CAGTCTACGC GAGCTGCCTG TTTTTTTCCT GCTTGGACGC GCATGAGGGC 51 CCCGTCCATG GACCGCGCGG CCGTGGCGAG GGTGGGCGCG GTAGCGAGCG 101 CCAGCGTGTG CGCCCTGGTG GCGGGGGTGG TGCTGGCTCA GTACATATTC ^ 151 ACCTTGAAGA GGAAGACGGG GCGGAAGACC AAGATCATCG AGATGATGCC 201 AGAATTCCAG AAAAGTTCAG TTCGAATCAA GAACCCTACA AGAGTAGAAG 251 AAATTATCTG TGGTCTTATC AAAGGAGGAG CTGCCAAACT TCAGATAATA 301 ACGGACTTTG ATATGACACT CAGTAGATTT TCATATAAAG GGAAAAGATG 351 CCCAACATGT CATAATATCA TTGACAACTG TAAGCTGGTT ACGGATGAAT 401 GTAGAAAAAA GTTATTGCAA CTAAAGGAAA AATATTACGC TATTGAAGTT 451 GATCCTGTTC TTACTGTAGA AGAGAAGTAC CCTTATATGG TGGAATGGTA 501 TACTAAATCA CATGGTTTGC TTGTTCAGCA AGCTTTACCA AAAGCTAAAC 551 TTAAAGAAAT TGTGGCAGAA TCTGACGTTA TGCTCAAAGA AGGATATGAG 601 AATTTCTTTG ATAAGCTCCA ACAACATAGC ATCCCCGTGT TCATATTTTC 651 GGCTGGAATC GGCGATGTAC TAGAGGAAGT TATTCGTCAA GCTGGTGTTT 701 ATCATCCCAA TGTCAAAGTT GTGTCCAATT TTATGGATTT TGATGAAACT 751 GGGGTGCTCA AAGGATTTAA AGGAGAACTA ATTCATGTAT TTAACAAACA 801 TGATGGTGCC TTGAGGAATA CAGAATATTT CAATCAACTA AAAGACAATA 851 GTAACATAAT TCTTCTGGGA GACTCCCAAG GAGACTTAAG AATGGCAGAT 901 GGAGTGGCCA ATGTTGAGCA CATTCTGAAA ATTGGATATC TAAATGATAG 951 AGTGGATGAG CTTTTAGAAA AGTACATGGA CTCTTATGAT ATTGTTTTAG 1001 TACAAGATGA ATCATTAGAA GTAGCCAACT CTATTTTACA GAAGATTCTA 1051 TAAACAAGCA TTCTCCAAGA AGACCTCTCT CCTGTGGGTG CAATTGAACT 1101 GTTCATCCGT TCATCTTGCT GAGAGACTTA TTTATAATAT ATCCTTACTC 1151 TCGAAGTGTT CCCTTTGTAT AACTGAAGTA TTTTCAGATA TGGTGAATGC 1201 ATTGACTGGA AGCTCCTTTT CTCCACCTCT CTCAACACAC TCCTCACCGT 1251 ATCTTTTAAC CCATTTAAAA AAAAAAAAAA AAAAA BLAST Results No BLAST result Medlme entries No Medline entry Peptide information for frame 1 ORF from 43 bp to 1050 bp; peptide length: 336 Category: similarity to unknown protein Classif cation: unset Prosite motifs: HTH LYSR FAMILY (16-47) 1 MRAPSMDRAA VARVGAVASA SVCALVAGVV LAQYIFTLKR KTGRKTKIIE 51 MMPEFQKSSV RIKNPTRVEE IICGLIKGGA AKLQIITDFD MTLSRFSYKG 101 KRCPTCHNII DNCKLVTDEC RKKLLQLKEK YYAIEVDPVL TVEEKYPYMV 151 EWYTKSHGLL VQQALPKAKL KEIVAESDVM LKEGYENFFD KLQQHSIPVF 201 IFSAGIGDVL EEVIRQAGVY HPNVKVVSNF MDFDETGVLK GFKGELIHVF 251 NKHDGALRNT EYFNQLKDNS NIILLGDSQG DLRMADGVAN VEHILKIGYL 301 NDRVDELLEK YMDSYDIVLV QDESLEVANS ILQKIL BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfkd2_46bl0, frame 1 SWISSPROT :YQT3_CAEEL HYPOTHETICAL 42.0 KD PROTEIN F25B5.3 IN CHROMOSOME III., N = 1, Score = 524, P = 2.2e-50 TREMBL :AC005499_12 gene: "T6A23.12"; Arabidopsis thaliana chromosome II BAC T6A23 genomic sequence, complete sequence., N = 2, Score = 194, P = 1.4e-26 >SWISSPROT:YQT3_CAEEL HYPOTHETICAL 42.0 KD PROTEIN F25B5.3 IN CHROMOSOME III. Length = 376 HSPs: Score = 524 (78.6 bits), Expect = 2.2e-50, P = 2.2e-50 Identities = 112/300 (37%), Positives = 174/300 (58%) Query: 44 RKTKIIEMMPEFQ—KSSVRIKNPTRVEEIICGLIKGGAAKLQIITDFDMTLSRFSYK-G 100 +KT ++ ++ + + + + +PT V + ++ GGA K +I+DFD TLSRF+ + G Sbjct: 73 KKTDVVPLLMNYLLGEEQILVADPTAVAAKLRKMVVGGAGKTVVISDFDYTLSRFANEQG 132 Query: 101 KRCPTCHNIID-NCKLVTDECRKKLLQLKEKYYAIEVDPVLTVEEKYPYMVEWYTKSHGL 159 +R T H + D N + E +K + LK KYY IE P LT+EEK P+M +W+ SH L Sbjct: 133 ERLSTTHGVFDDNVMRLKPELGQKFVDLKNKYYPIEFSPNLTMEEKIPHMEKWWGTSHSL 192 Query: 160 LVQQALPKAKLKEIVAESDVMLKEGYENFFDKLQQHSIPVFIFSAGIGDVLEEVIRQA-G 218 +V + K +++ V +S ++ K+G E+F + L H+IP+ IFSAGIG+++E ++Q G Sbjct: 193 IVNEKFSKNTIEDFVRQSRIVFKDGAEDFIEALDAHNIPLVIFSAGIGNIIEYFLQQKLG 252 Query: 219 VYHPNVKVVSNFMDFDETGVLKGFKGELIHVFNKHDGAL-RNTEYFNQLKDNSNIILLGD 277 N +SN + FDE F LIH F K+ + + T +F+ + N+ILLGD Sbjct: 253 AIPRNTHFISNMILFDEDDNACAFSEPLIHTFCKNSSVIQKETSFFHDIAGRVNVILLGD 312 Query: 278 SQGDLRMADGVANVEHILKIGYLNDRVDEL—LEKYMDSYDIVLVQDESLEVANSILQKI 335 S GD+ M GV LK+GY N +D+ L+ Y + YDIVL+ D +L VA 1+ I Sbjct: 313 SMGDIHMDVGVERDGPTLKVGYYNGSLDDTAALQHYEEVYDIVLIHDPTLNVAQKIVDII 372 Pedant information for DKFZphfkd2_46bl0, frame 1 Report for DKFZphfkd2_46bl0.1 [LENGTH] 336 [MW] 37948.37 [pi] 6.67 [HOMOL] SWISSPROT :YQT3_CAEEL HYPOTHETICAL 42.0 KD PROTEIN F25B5.3 IN CHROMOSOME III. 3e-51 [PROSITE] HTH_LYΞR_FAMILY 1 [KW] TRANSMEMBRANE 2 [KW] LOW COMPLEXITY 7.44 % SEQ MRAPSMDRAAVARVGAVASASVCALVAGVVLAQYIFTLKRKTGRKTKIIEMMPEFQKSSV SEG xxxxxxxxxxxxxxxxxxxxxxxxx PRD cccchhhhhcchhhhhhheeehhhhhhhhhhhhhhhhhhhhhccceeeehhhhhhhhhee MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM SEQ RIKNPTRVEEIICGLIKGGAAKLQIITDFDMTLSRFSYKGKRCPTCHNIIDNCKLVTDEC SEG PRD eecccchhhhhhhhhhccccceeeeecccccceeeecccccccccccccccccchhhhhh MEM SEQ RKKLLQLKEKYYAIEVDPVLTVEEKYPYMVEWYTKSHGLLVQQALPKAKLKEIVAESDVM SEG PRD hhhhhhhhhhhheeeccccccccccchhhhhhccccchhhhhhccchhhhhhhhhhhhcc MEM SEQ LKEGYENFFDKLQQHSIPVFIFSAGIGDVLEEVIRQAGVYHPNVKVVSNFMDFDETGVLK SEG PRD ccccchhhhhhhhhcccceeeeecccchhhhhhhhhhcccccceeeeeecccccccccee MEM MMMMMMMMMMMMMMMMMMM SEQ GFKGELIHVFNKHDGALRNTEYFNQLKDNΞNIILLGDSQGDLRMADGVANVEHILKIGYL SEG PRD eccceeeeeeecccccccccchhhhhhhhceeeeecccccccccccccccccceeeeeec MEM SEQ NDRVDELLEKYMDSYDIVLVQDESLEVANSILQKIL SEG PRD cchhhhhhhhhhhhheeeeeecchhhhhhhhhhccc MEM Prosite for DKFZphfkd2_46bl0.1 PS00044 16->47 HTH_LYSR_FAMILY PDOC00043 (No Pfam data available for DKFZphfkd2_46bl0.1) DKFZphfkd2_46dl3 group: kidney derived DKFZphfkd2_46dl3 encodes a novel 506 amino acid protein with weak similarity to KE03 protein The novel protein contains a RGD site. No informative BLAST results; No predictive prosite, pfam or SCOP motive The new protein can find application in studying the expression profile of kidney-specific genes . similarity to KE03 protein complete cDNA, complete eds, EST hits Sequenced by MediGenomix Locus: /map="227.6 cR from top of Chrl linkage group" Insert length: 3346 bp Poly A stretch at pos. 3328, polyadenylation signal at pos. 330E 1 CTCTCGCGAG AGGAGCAAGA GGAAGATGGC CGTGCCCTGT TTTTCGGTGT 51 AAGGCAGCAG ACGGCGGCTG CGACGGCGAG ACTGAGATCC TGGTGTCGTG 101 GGCACCTGAG TTCTAGCTTC CCCCAGCGAG CGCGCGTCCC TTCGTGCCTA 151 GGCGAGAGCC GGCTCTTCCC CGGGAGATGC GTTTGTCCCA GGCTCGGGGG 201 CTCAGTGGGA GTTCATGCTG CGCTGGAGGC TCTTGGCCAC CGCTCTAATC 251 GCCTTGTGCC GCCGCAGCGC CAGCTCCGTC GCCAGCGGTG AGCCTCCCGA 301 TTCCCCCCCT TGCCCCTGGC GGCGGCGATG ACCGGGGAGA AGATCCGCTC 351 ACTGCGGAGG GACCACAAGC CCAGCAAAGA AGAAGGGGAC CTGCTGGAGC 401 CCGGGGATGA AGAAGCGGCG GCTGCCCTCG GCGGTACCTT TACCAGAAGC 451 AGGATTGGCA AGGGCGGCAA AGCTTGTCAT AAGATCTTCA GTAACCATCA 501 CCACCGGCTA CAGCTGAAGG CAGCTCCGGC CTCCTCCAAT CCCCCCGGCG 551 CCCCGGCTCT GCCGCTGCAC AATTCCTCCG TGACTGCCAA CTCCCAGTCC 601 CCGGCCCTTC TGGCCGGCAC CAACCCCGTT GCTGTCGTCG CGGATGGAGG 651 CAGTTGCCCC GCACACTACC CGGTGCACGA GTGCGTCTTC AAGGGGGATG 701 TGAGGAGACT CTCCTCTCTC ATCCGCACGC ACAATATCGG GCAGAAAGAT 751 AATCACGGAA ATACTCCTTT ACACCTTGCT GTGATGTTAG GAAATAAAGT 801 TACAGCTCTT TTGAGGAAGC TTAAGCAGCA ATCCAGGGAA AGTGTTGAAG 851 AAAAACGACC TCGATTATTA AAAGCCCTGA AAGAGCTAGG TGACTTTTAT 901 CTAGAACTTC ACTGGGATTT TCAAAGCTGG GTGCCTTTAC TTTCCCGAAT 951 TCTGCCTTCC GATGCATGTA AAATATACAA ACAAGGTATC AATATCAGGC 1001 TTGACACAAC TCTCATAGAC TTTACTGACA TGAAGTGCCA ACGAGGGGAT 1051 CTAAGCTTCA TTTTCAATGG GGATGCGGCG CCCTCTGAAT CTTTTGTAGT 1101 ATTAGACAAT GAACAAAAAG TTTATCAGCG AATACATCAT GAGGAATCAG 1151 AGATGGAAAC AGAAGAAGAG GTGGATATTT TAATGAGCAG TGATATTTAC 1201 TCTGCAACTT TATCAACAAA ATCAATTTCT TTCACGCGTG CCCAGACAGG 1251 ATGGCTTTTT CGGGAAGATA AAACAGAAAG AGTAGGAAAC TTTTTGGCAG 1301 ACTTTTACCT GGTGAATGGA CTTGTTATAG AATCAAGGAA AAGAAGAGAA 1351 CATCTCAGTG AAGAGGATAT TCTTCGAAAT AAGGCCATCA TGGAGAGTTT 1401 GAGTAAAGGT GGAAACATAA TGGAACAGAA TTTTGAGCCG ATTCGAAGAC 1451 AGTCTCTTAC ACCGCCTCCT CAGAACACTA TTACATGGGA AGAATATATA 1501 TCTGCTGAAA ATGGAAAAGC TCCTCATCTG GGTAGAGAAT TGGTGTGCAA 1551 AGAGAGTAAG AAAACGTTTA AAGCTACGAT AGCCATGAGC CAGGAATTTC 1601 CCTTAGGGAT AGAGTTATTA TTGAATGTTT TAGAAGTAGT AGCTCCCTTC 1651 AAGCACTTTA ACAAGCTTAG AGAATTTGTT CAGATGAAGC TTCCTCCAGG 1701 CTTTCCTGTA AAATTAGATA TACCTGTGTT TCCCACAATC ACAGCCACTG 1751 TGACTTTTCA GGAGTTTCGA TACGATGAAT TTGATGGCTC CATCTTTACT 1801 ATACCTGATG ACTACAAGGA AGACCCAAGC CGTTTTCCTG ATCTTTAACT 1851 GACGTGGAAA AGGATGCCGT CTAACCAAGG AAAGAAAATA CAGAGACCCT 1901 AGAAGTGGAT CCAAATAGAA GGGACAAATG CTTTCAGTGA AGAAAAGGGA 1951 ATTACACATT GAATCGACAC ATCAGTAATA CGATACAGTG AAATGGGCCT 2001 CTAATAAGAA TTTCAGCGAG TTTTCTGATG TGCCATTTTT TGTCTTTTTA 2051 AAAATATACA TATTATAAAT GTAATAGTTT GACACATTAA TGACCCTAAG 2101 ACCTGCGTAT GTGAAGCAGC TATGAGTGCT GTGATTTGTT TTTAAAAATT 2151 TTTACACTTC TTGTTGAAAT ATATATGCAT ATAAATATAT CTATATCTAT 2201 ATCTATATCT AAAACACTCC TGGACCATTA ACGTAAATTA AATGTCTTAA 2251 GAGATATGGA GCCCTTTTAA ACTTGTCATC TTTATGCAAG GTGACATTTA 2301 TAAATATTCC TTCGAGCTTT GTTTTCATAA AATGTAAACT ATGTAACATT 2351 ATGTATAGTT CAGTAATTTG AATGTTTGTT CAATATAATG AACTAGAAGG 2401 AATGCAATTT TCTGTAGATG AATGAACCAA ATGGTAACCA TTAAACAATT 2451 GCATTTATAT GTTGCAATAC ATTTCAGAAG GAGCGTTCAC TCTGCAGGGA 2501 ATAAGGTACC TCCTTTAGCA CCTTAGTGCA ATTCATTGTG GTGCTATTTG 2551 TTTTTACCTG AATGTTTGTT ACTAATCTTC CTTTCATAGA ACCTCTATTT 2601 TTTTTTTTTC TAAACTTGAG TTTGAGTCCT TGTTATGGTC ATCATAAGGT 2651 AATGGTTAGC ATGTTTAAAG ATATTCCTCT TCCAAATCTC AGCACTTTAA 2701 AAAAAAATCC AAATTTTTAA ACTTGCTTCC TAATAAGTAC ACATCGGTCT 2751 GATTATTTTG TTTGTTTTTA GTAGAATATG GATGCATTGG TGTCAGTTTT 2801 AAAAAACAAT ACACATATTT TGGACAACCC TACATATTTA ATCCTTTCAA 2851 AATAAGATAA AAACATTTTA TATGCTAACA GAATATATTT GTTACAAGTT 2901 AAAGTCCAGA AGTATACACA AGATTGATTA CTCCTATTAT TTTTTTTAAA 2951 TCACAGGAAA ATATTGATTT CATTGTCTCC AAAGTGATAA AATCTTGTAT 3001 TACTCATTTT TGCACTTAAA ATTTTTCTTA TTTATTCCAA GGTGGTTTGA 3051 AGGTCCAAGT ATGAAAATAA ATTAGGGGGA TTAATGTATA ACAGTTATAA 3101 AGTATCATGT TGTATTAAAG AGCTTACTTA GATTGATGTT TTTAAAATGT 3151 ATCCTGATGA ATGTCTCAAG AATGCATCTG TCAAGTTTTT TAGACTGACC 3201 AGTAGCTTAA ACTTTTTTCA GGATTTTAGG TAATTTGAAA GGAGTTTAGA 3251 GACCCTTATT GAAAATATGA TTTAAAAATC CAAAGCATAA ACCGTAAGAA 3301 AAATTTTAAA TAAACATCTT TAAAGCTGAA AAAAAAAAAA AAAAAA BLAST Results Entry HS121353 from database EMBL: human STS WI-14729. Score = 1697, P = 1.9e-69, identities 363/379 Medline entries No Medline entry Peptide information for frame 1 ORF from 328 bp to 1845 bp; peptide length: 506 Category: similarity to unknown protein 1 MTGEKIRSLR RDHKPSKEEG DLLEPGDEEA AAALGGTFTR SRIGKGGKAC 51 HKIFSNHHHR LQLKAAPASS NPPGAPALPL HNSSVTANSQ SPALLAGTNP 101 VAVVADGGSC PAHYPVHECV FKGDVRRLSS LIRTHNIGQK DNHGNTPLHL 151 AVMLGNKVTA LLRKLKQQSR ESVEEKRPRL LKALKELGDF YLELHWDFQS 201 WVPLLSRILP SDACKIYKQG INIRLDTTLI DFTDMKCQRG DLSFIFNGDA 251 APSESFVVLD NEQKVYQRIH HEESEMETEE EVDILMSSDI YSATLSTKSI 301 SFTRAQTGWL FREDKTERVG NFLADFYLVN GLVIESRKRR EHLSEEDILR 351 NKAIMESLSK GGNIMEQNFE PIRRQSLTPP PQNTITWEEY ISAENGKAPH 401 LGRELVCKES KKTFKATIAM SQEFPLGIEL LLNVLEVVAP FKHFNKLREF 451 VQMKLPPGFP VKLDIPVFPT ITATVTFQEF RYDEFDGSIF TIPDDYKEDP 501 SRFPDL BLASTP hits Entry CEC01F1_3 from database TREMBL: gene: "C01F1.6"; Caenorhabditis elegans cosmid C01F1. Score = 371, P = 4.5e-61, identities = 69/138, positives = 96/138 Entry CEC18F10_9 from database TREMBL: gene: "C18F10.7"; Caenorhabditis elegans cosmid C18F10. Score = 383, P = 3.4e-39, identities = 103/349, positives = 182/349 Entry AF064604_1 from database TREMBL: product: "KE03 protein"; Homo sapiens KE03 protein mRNA, partial eds. Score = 348, P = 8.3e-32, identities = 95/295, positives = 148/295 Alert BLASTP hits for DKFZphfkd2_46dl3, frame 1 No Alert BLASTP hits found Pedant information for DKFZphf d2_46dl3, frame 1 Report for DKFZphfkd2_46dl3.1 [LENGTH] 506 [MW] 57003.12 [pi] 6.40 [HOMOL] TREMBL :CEC18F10_9 gene: "C18F10.7"; Caenorhabditis elegans cosmid C18F10. 2e-35 [BLOCKS] BL01288E [PROSITE] RGD 1 [PROSITE] MYRISTYL 7 [PROSITE] CAMP_PHOSPHO_SITE 2 [PROSITE] CK2_PHOΞPHO_SITE 9 [PROSITE] PKC_PHOSPHO_SITE 6 [PROSITE] ASN_GLYCOΞYLATION 1 [KW] Alpha_Beta [KW] LOW COMPLEXITY 7.51 ' SEQ MTGEKIRSLRRDHKPSKEEGDLLEPGDEEAAAALGGTFTRSRIGKGGKACHKIFSNHHHR SEG xxxxxxxxxxxx PRD ccceeeeeccccccccccccccccccchhhhhhhccccccccccccceeeeeeecchhhh SEQ LQLKAAPASSNPPGAPALPLHNSSVTANSQSPALLAGTNPVAVVADGGSCPAHYPVHECV SEG .... xxxxxxxxxxxxxxxx PRD hhhhhhccccccccceeecccccccccccccceeecccccceeeecccccccccccceee SEQ FKGDVRRLSSLIRTHNIGQKDNHGNTPLHLAVMLGNKVTALLRKLKQQSRESVEEKRPRL SEG PRD eccchhhhhhhhhhcccccccccccccceeeecccchhhhhhhhhhhhcchhhhhhhhhh SEQ LKALKELGDFYLELHWDFQSWVPLLSRILPSDACKIYKQGINIRLDTTLIDFTDMKCQRG SEG PRD hhhhhhccccceeehhhhhccceeeeccccccceeeeeccceeeeeeeeecccccccccc SEQ DLSFIFNGDAAPSESFVVLDNEQKVYQRIHHEESEMETEEEVDILMSSDIYSATLSTKSI SEG xxxxxxxxxx PRD ceeeeeccccceeeeeeeecccceeeehhhhhhhhhhhhhhhhhhhhccceeeecccccc SEQ SFTRAQTGWLFREDKTERVGNFLADFYLVNGLVIESRKRREHLSEEDILRNKAIMESLSK SEG PRD eeeecccceeeecccchhhhhhheeeeeeeeeeeeehhhhhhhhhhhhhhhhhhhhhhhc SEQ GGNIMEQNFEPIRRQSLTPPPQNTITWEEYISAENGKAPHLGRELVCKESKKTFKATIAM SEG PRD cceeeccccccccccccccccccccccccccccccccccccccccchhhhhhhhhhhhhh SEQ SQEFPLGIELLLNVLEVVAPFKHFNKLREFVQMKLPPGFPVKLDIPVFPTITATVTFQEF SEG PRD hhcccchhhhhhhhhhhhhhhhhhhhhhhhhhhhcccccceeeeeeeeeeehhhhhhhcc SEQ RYDEFDGSIFTIPDDYKEDPSRFPDL SEG PRD cccccccceeeccccccccccccccc Prosite for DKFZphfkd 2 _46dl3.1 PS00001 82->86 ASN_GLYCOSYLATION PDOC00001 PS00004 126->130 CAMP_PHOSPHO_SITE PDOC00004 PS00004 373->377 CAMP_PHOSPHO_SITE PDOC00004 PS00005 8->ll PKC_PHOSPHO_SITE PDOC00005 PS00005 296->299 PKC_PHOSPHO_SITE PDOC00005 PS00005 316->319 PKC_PHOSPHO_SITE PDOC00005 PS00005 336->339 PKC_PHOSPHO_SITE PDOC00005 PS00005 410->413 PKC_PHOSPHO_SITE PDOC00005 PS00005 413->416 PKC_PHOSPHO_SITE PDOC00005 PS00006 16->20 CK2_PHOSPHO_SITE PDOC00006 PS00006 172->176 CK2_PHOSPHO_SITE PDOC00006 PS00006 228->232 CK2_PHOSPHO_SITE PDOC00006 PS00006 274->278 CK2_PHOSPHO_SITE PDOC00006 PS00006 278->282 CK2_PHOSPHO_SITE PDOC00006 PS00006 344->348 CK2_PHOSPHO_SITE PDOC00006 PS00006 386->390 CK2_PHOSPHO_SITE PDOC00006 PS00006 476->480 CK2_PHOSPHO_SITE PDOC00006 PS00006 491->495 CK2_PHOSPHO_ΞITE PDOC00006 PS00008 35->41 MYRISTYL PDOC00008 PS00008 46->52 MYRISTYL PDOC00008 PS00008 108->114 MYRISTYL PDOC00008 PS00008 138->144 MYRISTYL PDOC00008 PS00008 155->161 MYRISTYL PDOC00008 PS00008 320->326 MYRISTYL PDOC00008 PS00008 487->493 MYRISTYL PDOC00008 PS00016 239->242 RGD PDOC00016 (No Pfam data available for DKFZphfkd2_46dl3.1) DKFZphfkd2_46j20 group: metabolism DKFZphfkd2_346j20 encodes a novel 224 amino acid protein similar to 2-hydroxyhepta-2, 4-dιene- 1,7-dιoate isomerase. The new protein seems to be the human ortholog of 2-hydroxyhepta-2, 4-dιene-l, 7-dιoate isomerase . The new protein can find application in modulating the homoprotocatechuate degradative pathway and as a enzyme for biotechnologic production processes. strong similarity to 2-hydroxyhepta-2, 4-dιene-l, 7-dιoate isomerase complete cDNA, complete eds, EST hits, potential start at Bp 16 matches kozak consensus ANCatgG strong similarity to proteins of worm plant archea and bacteria 2-hydroxyhepta-2, 4-dιene-l, 7-dιoate isomerase is part of the tyrosine metabolism (degradation of tyrosine late step) EC 5.3.1.- complete eds according to similar C. elegans and A. thaliana protein Sequenced by MediGenomix Locus : unknown Insert length: 1706 bp Poly A stretch at pos. 1686, polyadenylation signal at pos. 1667 1 CACTTGATGG GAATCATGGC AGCATCCAGG CCATTGTCCC GCTTCTGGGA 51 GTGGGGAAAG AACATCGTCT GCGTGGGGAG GAACTACGCG GACCACGTCA 101 GGGAGATGCG CAGCGCGGTG TTGAGCGAGC CCGTGCTGTT CCTGAAGCCG 151 TCCACGGCCT ACGCGCCCGA GGGCTCGCCC ATCCTCATGC CCGCGTACAC 201 TCGCAACCTG CACCACGAGC TGGAGCTGGG CGTGGTGATG GGCAAGCGCT 251 GCCGCGCAGT CCCCGAGGCT GCGGCCATGG ACTACGTGGG CGGCTATGCC 301 CTGTGCCTGG ATATGACCGC CCGGGACGTG CAGGACGAGT GCAAGAAGAA 351 GGGGCTGCCC TGGACTCTGG CGAAGAGCTT CACGGCGTCC TGCCCGGTCA 401 GCGCGTTCGT GCCCAAGGAG AAGATCCCTG ACCCTCACAA GCTGAAGCTC 451 TGGCTCAAGG TCAACGGCGA ACTCAGACAG GAGGGTGAGA CATCCTCCAT 501 GATTTTTTCC ATCCCCTACA TCATCAGCTA TGTTTCTAAG ATCATAACCT 551 TGGAAGAAGG AGATATTATC TTGACTGGGA CGCCAAAGGG AGTTGGACCG 601 GTTAAAGAAA ACGATGAGAT CGAGGCTGGC ATACACGGGC TGGTCAGTAT 651 GACATTTAAA GTGGAAAAGC CAGAATATTG AGTTATTTCT TAACAAGTTT 701 CGAGAGAGAA GGGAGCAAGA CAAGAGCAAG CAACGGCTAT TAAATGTCAC 751 AATCCTTTAA TTAGAAACCA TTTATTGGCC GGACGCGGTG GCTCACGCCT 801 GTAATCGCAG CACTTTGGGA GGCCGAGGCG GGCGGCTCAC GACGTCAGGA 851 GATCCAGACC ATCTTGGCTA ACAGGGTGAA ACCCCGTCTC TACTAAAAAT 901 ACAAAAAATT AGCCGGGCGT GGTGGCGGGC GCCTGTAGTC CCAGCTACTC 951 TGGAGGCTGA GGCAGGAGAA TCAATTGAAC CCGGGAGGCG GAGCTTACAG 1001 TGAGCTGAGA TTGCGCCACT GTACTCCTGG GCAACAGCGA GACTCCGTCT 1051 CAAAAAAAAA AAAAAAAAAA AGAAACCATT TATTTTAAAA ATGATTAGAT 1101 TGCTATGCCT CAACTCATAG AAGATGAACC CTTCAAGAAA ACGTGAAGTA 1151 GAACGGGTGG GCCAGAAATG AAAACAGGCA AGTAAAGTAT TTCTTCGGAA 1201 AACATTTTAT CAAACCAAAT GTTAAAAAGA CTTTCCTTTT GTAAAACTGG 1251 ATTAGAGAAG ACTTTTCAGT GGGTTATCTC TAGGATGATC AGTAGTTCAG 1301 CACTTAAAAA CTGCAGAGAA AACTGAAAGT TATGTTCCAG ATAACTTTCC 1351 GTTGTTTACC AAATTTTCTT AGATTTGGTC ATCATCAGGA AGCATTTGTA 1401 AAAATAAAAA TCTCCACAAA TTACTGGCCC ATCTCGGACT TGCTGAATCA 1451 ATTTGATAGG ATTAATCTCC AGTGAAGCTG TGTTTACAGG GCATTCCAAG 1501 TGATTCTTAT CAGGAAATGT GAAAAACACT CCTGTACATA ATCGGTTAAT 1551 TTAAAATTTT ACTTAATAAG TGAACAAGTA ATGAAGATTT CACCTGTTTA 1601 CTTAGGGTAT CTACCCAGAC CCATCGATTC TGAGTTCGGG AGATGATTTT 1651 GAAATTACTG TTTTCCAAAT AAAGGTGCTC CCTTCCAAAA AAAAAAAAAA 1701 AAAAAA BLAST Results No BLAST result Medline entries 94039092: Purification, nucleotide sequence and some properties of a bifunctional isomerase/deearboxylase from the homoprotocatechuate degradative pathway of Escherichia coli C. Peptide information for frame 1 ORF from 7 bp to 678 bp; peptide length: 224 Category: strong similarity to known protein 1 MGIMAASRPL SRFWEWGKNI VCVGRNYADH VREMRSAVLS EPVLFLKPST 51 AYAPEGSPIL MPAYTRNLHH ELELGVVMGK RCRAVPEAAA MDYVGGYALC 101 LDMTARDVQD ECKKKGLPWT LAKSFTASCP VSAFVPKEKI PDPHKLKLWL 151 KVNGELRQEG ETΞSMIFSIP YIISYVSKII TLEEGDIILT GTPKGVGPVK 201 ENDEIEAGIH GLVSMTFKVE KPEY BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfkd2_46j20, frame 1 PIR:S44919 ZK688.3 protein - Caenorhabditis elegans, N = 1, Score = 537, P = 8.7e-52 PIR:D71109 probable 2-hydroxyhepta-2, 4-dιene-l, 7-dιoate isomerase - Pyrococcus horikoshn, N = 1, Score = 529, P = 6.1e-51 PIR:C71425 hypothetical protein - Arabidopsis thaliana, N = 1, Score = 519, P = 7e-50 PIR:A64864 probable 2-hydroxyhepta-2, 4-dιene-l, 7-dιoate isomerase bll80 - Escherichia coli, N = 1, Score = 474, P = 4.1e-45 >PIR:S44919 ZK688.3 protein - Caenorhabditis elegans Length = 214 HSPs: Score = 537 (80.6 bits), Expect = 8.7e-52, P = 8.7e-52 Identities = 99/211 (46%), Positives = 138/211 (65%) Query: 10 LSRFWEWGKNIVCVGRNYADHVREMRSAVLSEPVLFLKPSTAYAPEGSPILMPAYTRNLH 69 L+ F IVCVGRNY DH E+ +A+ +P+LF+K ++ EG PI+ P +NLH Sbjct: 4 LAGFRNLATKIVCVGRNYKDHALELGNAIPKKPMLFVKTVNSFIVEGEPIVAPPGCQNLH 63 Query: 70 HELELGVVMGKRCRAVPEAAAMDYVGGYALCLDMTARDVQDECKKKGLPWTLAKSFTASC 129 E+ELGVV+ K+ + ++ AMDY+GGY + LDMTARD QDE KK G PW LAKSF SC Sbjct: 64 QEVELGVVISKKASRISKSDAMDYIGGYTVALDMTARDFQDEAKKAGAPWFLAKSFDGSC 123 Query: 130 PVSAFVPKEKIPDPHKLKLWLKVNGELRQEGETSSMIFSIPYIISYVSKIITLEEGDIIL 189 P+ F+P IP+PH ++L+ K+NG+ +Q T MIF IP ++ Y ++ TLE GD++L Sbjct: 124 PIGGFLPVSDIPNPHDVELFCKINGKDQQRCRTDVMIFDIPTLLEYTTQFFTLEVGDVVL 183 Query: 190 TGTPKGVGPVKENDEIEAGIHGLVΞMTFKVE 220 TGTP GV + D IE G+ ++ F V+ Sbjct: 184 TGTPAGVTKINSGDVIEFGLTDKLNSKFNVQ 214 Pedant information for DKFZphfkd2_46j20, frame 1 Report for DKFZphf d2_46j 20.1 [LENGTH] 224 [MW] 24843.07 [pi] 6.96 [HOMOL] PIR:S44919 ZK688.3 protein - Caenorhabditis elegans 8e-55 [FUNCAT] r general function prediction [M. jannaschn, MJ1656] 9e-40 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YNL168c] 4e-38 [EC] 5.3.3.10 5-Carboxymethyl-2-hydroxymuconate delta-isomerase le-35 [PIRKW] isomerase le-35 [PIRKW] intramolecular oxidoreductase le-35 [SUPFAM] 2-hydroxyhepta-2, 4-dιene-l, 7-dιoate isomerase le-46 [PROSITE] MYRISTYL 4 [PROSITE] AMIDATION 1 [PROSITE] CK2_PHOSPHO_SITE 2 [PROSITE] PKC_PHOSPHO_SITE 3 [KW] Alpha_Beta SEQ MGIMAASRPLSRFWEWGKNIVCVGRNYADHVREMRSAVLSEPVLFLKPSTAYAPEGSPIL PRD cccccccccchhhhhhcceeeeeecchhhhhhhhhccccccceeeecccccccccccccc SEQ MPAYTRNLHHELELGVVMGKRCRAVPEAAAMDYVGGYALCLDMTARDVQDECKKKGLPWT PRD cccccchhhhhhheeeccccccccchhhhhhhheeeeeeccchhhhhhhhhhhhcccccc SEQ LAKSFTASCPVSAFVPKEKIPDPHKLKLWLKVNGELRQEGETSSMIFSIPYIISYVSKII PRD cccccccccccceeeecccccccccceeeeecccccccccccccceeechhhhhhhhhhh SEQ TLEEGDIILTGTPKGVGPVKENDEIEAGIHGLVSMTFKVEKPEY PRD hccccceeeeccccccccccccceeeeeeccccccccccccccc Prosite for DKFZphfkd2_46j 20.1 PS00005 104->107 PKC_PHOSPHO_SITE PDOC00005 PS00005 192->195 PKC_PHOSPHO_SITE PDOC00005 PS00005 216->219 PKC_PHOSPHO_SITE PDOC00005 PS00006 104->108 CK2_PHOSPHO_SITE PDOC00006 PS00006 181->185 CK2_PHOSPHO_SITE PDOC00006 PS00008 2->8 MYRISTYL PDOC00008 PS00008 75->81 MYRISTYL PDOC00008 PS00008 116->122 MYRISTYL PDOC00008 PS00008 191->197 MYRISTYL PDOC00008 PS00009 78->82 AMIDATION PDOC00009 (No Pfam data available for DKFZphfkd2_46 20.1) DKFZphfkd2_46kl9 group: transcription factors DKFZphfkd2_46kl9.3 encodes a novel 130 ammo acid protein similar to rat Dcoh, a bifunctional protein-bmding transcriptional co-activator . Dcoh is a bifunctional protein, complexed with biopterin. It serves as dimerization cofactor of hepatocyte nuclear factor-1 and catalyzes the dehydration of the biopterin cofactor of phenylalanine hydroxylase . The new protein can find application in modulating/blocking the expression of genes controlled by the hepatocyte nuclear factor-1. strong similarity to pterm-4-alpha-carbιnolamιne dehydratase potential start at Bp 102 according to similar proteins, both genomic sequences are from chromosome 5, Sequenced by MediGenomix Locus: map="5" Insert length: 5641 bp Poly A stretch at pos. 5617, polyadenylation signal at pos. 5598 1 CAGCCCTCGG CAGACGGCCA ATGGCGGCGG TGCTCGGGGC GCTCGGGGCG 51 ACGCGGCGCT TGTTGGCGGC GCTGCGAGGC CAGAGCCTAG GGCTAGCGGC 101 CATGTCATCA GGTACTCACA GGTTGATTGC AGAGGAGAGG AACCAAGCTA 151 TACTTGACCT TAAAGCAGCA GGATGGTCGG AATTAAGTGA GAGAGATGCC 201 ATCTACAAAG AATTCTCCTT CCACAATTTT AATCAGGCAT TTGGCTTTAT 251 GTCCCGAGTT GCCCTACAAG CAGAGAAGAT GAATCATCAC CCAGAATGGT 301 TCAATGTATA CAACAAGGTC CAGATAACTC TCACCTCACA TGACTGTGGT 351 GAACTGACCA AAAAAGATGT GAAGCTGGCC AAGTTTATTG AAAAAGCAGC 401 TGCTTCTGTG TGATTTCTTC CAAAATACAT AAGTCTGAGA GGCTAAACTT 451 GATGGCTGTG TTAACATATG TCACGTGTAG CACAGTGGAG AAAGCAGGAT 501 ATGGCTCATA ATGACAGTGG TGAAGACCTG CGAATGAAGT TGCTAGTTAA 551 CACCTACATT AGGGTTTGAC ATAGGTCTAT GTTATGGGTC GCTGCATCTG 601 CTGGAACTCA CAGACTTTAC TATAGAGAAT CAAAGATCCC GTATCCGAAG 651 TCTATGGAAA TGCTCATGGT GGTAAATTCC AACAGAATGA AACACCAAAC 701 TTGCTTAAAG TAACTCACGT TTCAATTTGA AAGAGATATT GTCAAAATTG 751 GAGGCCCCCA GGTTCCTGTC TGTTCCAAAT CTTTGCATGA TGACAGTGGT 801 TTCTCTGATG TGGTAAGCTT TGGCTTTCTT CTGTTTTCTT TCTAAAAGAT 851 CACTGGAGTA GAGAGGAGTT AAACAGACAT GACCTTTGAC CTCTTGCATG 901 ACCTCCACAG ATAGCAAACC GGGCCGACAC ATGGTTGACG ATGTCCTTTT 951 CTACAATGAA GTTAATGAAA GTTCTGAAAA TAGTGATTAC TTTCTGACAT 1001 TGATAGGATT TAGGAAACCT CTGGATAAAT AGCTTAAGCA TGGCTGTTTA 1051 TGTTTTTGCT ATAGACAAAA AGCAGCAGCA TGTACATTGT ATTTGGACAC 1101 AAGCCTGCCT CGGTTAATAT ATTGAACTAT TGGACCACTA GGGTTAGTAG 1151 GGAGCGGTCT GTACACTTTC TGATTCAGCA TTCAGAAACA TTCTAGGTGG 1201 ACTCTGTAGC TTTCAGTTTT GTAAAGTTAT CGGAAAAACA TCGGGAGGGT 1251 TTGGCCATCA TATGTGAGCT TTGTGTTTCA ATGCCAGTTA CTCAGGATTA 1301 GTAAATTAAT GACTGTCCAG AGGACTTCAG GGTCACCAAG CTGCTGCACC 1351 TGCCATTGGC TGACTCTCCC CGGCTATCTG TGGCTGAGAT GGTGCTGCTT 1401 AGGTCACGCA GAGCATGAGC TGCTGCTGAA AGGGCACAGG AGATGGCCCT 1451 TGGGCTTCTC ATCCCAGGAT GCCTGCCCTG CCCACCAATC CATGAGAAGA 1501 TATGTATGAT TTCAGTAGGC CCTGGATCAG CTTGTCACCT CTGGTTTCCT 1551 GTTTGCTTTC CACTCACTCA GCTGGAGTTT CATTTCCAGA CTAAAGTCTT 1601 CATCATTGGC TTCAGAAACA GCATTCATCT GTGGCTGTGC TGATGTAGTA 1651 CACCAAGAAC AACTGGGCTC TTCTCTGTCA CTTTCAGTGG GCTACCTTCC 1701 CTCACCTCTC CAAGCAGCAT GAAAGAATTC TTTACATTTT TAATCTCTTT 1751 TTTGTTTTTC CCTGAAAGTA TGCTTTGGTG CTTAAAGAGA GAAGTCACAA 1801 AAGTATACTA CTGAGTTTCC TGGAGATGAA ATCCTGTTGT CCCTAGCTAT 1851 GTGAATGAGC ACAGGGATCC CTGATGCCAT TATTTTGTAT ATTCATACGG 1901 CACACACTTA CTGAGGGCCT TCTGTGTGCC CTAGGGGATT GAGCACAGTG 1951 ACATATCAGG GCAGGTAGAA ACAGATGGAG AGCTGATGCG GGCTGTCTTA 2001 GAGCAGCTGC CCCAGGAGGC CCCTGTGGAT GGATGTTGGG CAGGAGCCCT 2051 GAGACGTTAG GGGCATATAA CTAAAGGACA TAGCAGGAGT TATAGGAGGA 2101 GCTGATCCCT GAGGGAAACA ATGAAGACGG AGAAGATGGG GCTAAAGTTT 2151 GAATTGTGGG GACATTAATC ACGGTGATTC TTAAAACTTT GCTGTTGATG 2201 ATTTTAAATG GAGAAAATGA GTACGTAAGA TGTTATTTCC CAGTTCAGTA 2251 TATAGGTTGC CCACAAAGTA TTTTCCTACC ATGAATGGTC ATATATACTT 2301 GTTGTAGAAT ACCAGGGACA GCAGAGATGG TGGGGTAGTT ACTTCCTTTT 2351 CTTACAGCCC AAGAACTTTG GTGTCCAGGA GATTGACCAA TTTAGCCACT 2401 GAGCATTTAA TACAACACAG GGCTACCCAG ATCCCACTGT CCTGATTTGC 2451 CCTGAAAGCC AAAGGAGTCA GGAGAAGGTG AGTGGGGTGA ATATATTAAT 2501 CCTGAGAGTT GAACAGAGCA AAAATCCCTA TTACTTTTGT ACTTAAAACA 2551 TCTCTGCCAC ATGTGCTCAC TCTTTATATT CTGTTTAGGT GGTTTATATG 2601 TGCACATCCC ATCCTATGCC TGCAGTTAGC CAACTCAGGG TTTATATTGC 2651 CTCCTTTCTT TTTTTCTTTT TTTTAAGAGA TGGGGTCTCG 2701 TTCTGTCATG CAGACTGGAG TGCAGTGGTG TGATCACAGC TCATTGTAAC 2751 CTCCAACGCC TGGACTGAAG TGATCCTCCT GCCTTGGCCT CTCTGGTAGC 2801 TGGGACTACA GGTGCATGCC ACCACACCCA CCTAATTTTT TTTATTTTTA 2851 TTTTTTGTAG AGACAGTCTC ACTATCTTGC TCGGGCTGGT CCTGAACTCC 2901 TGGGCTCAAG TTATCTTGCT GCCTCAGCCT CCCATGGGTA ATCTTTATTT 2951 CCTTTTTTTT TTTTTTTTGG AGATGGAGTT TCGCTCTTGT CGCCCAGGCT 3001 GGAGTGCAAT GGCACGATCT TGGCTCACTG CAGTCTCCAC CTCCTGGGTT 3051 CAGGTGATTC TCCATCCTCG GCCTACTGAG TAGCTGAGAT TACAGGCAAC 3101 TGCCACCATG CGCGGCTAAT TTGTGTATTT TTTTTTAGTA AGAGATGGGG 3151 TTTCGCCATG TTGGCCGGAC TGGTCTTAGA CTCCTGACCT CAAGCGACCT 3201 GCCTGCCTTG GCCTCCCAAA GTGCTGGGAT TACAGGCATG AGCCGCTATG 3251 CCTCGTCGCT GATTTTTATT TCTTATTTTT TTTTTAGAGA TGGGGGTCTC 3301 ACTATGCTGC TCAGGCTGAT CTCAAACTCC TGGCCTCAAG TGATCCTCCC 3351 ACCTTAGCCT CCCAAGTTGC TGGGATTATA AGTGTGAGCC ACTATCCCTA 3401 CCTCACTATT ACCTTCTTTG CTTCTCTTGT TTTCTTTTGT TCTAAGTCAA 3451 ACCCATCACA ATCTTTTCTT GTCCTTCCAG GTGTTTTCCA GTGCTGTGCC 3501 CTGGATGTGC TCTCTTTCTC TTAGAGCCCA GAGAACTTGC TTTTCCCCCT 3551 TATATATGAC CCTTAACTTT TTCTAACACA TTATTAAGGG CCTGTGTCTA 3601 TCAGCTGGGG GCACTTCTTG AAGGGAGGGC CTTTGTGTGG TCTGTTTCTA 3651 GTGACTTCCA GCTTTAACCC AGAGCCTCAT GATTGCTGGG TGCCCATAGC 3701 CTTTTTGCTG AATGGAGGCA CTCAGTCTCC TTGGGAAGAG AGAATCCATG 3751 ATAGACCCAC TTGGGAGCTC CCCACTTCAG GGGCCTACAC ACTGGTAATG 3801 CAACAGAATG CCCAAGAGTG ACCTCATAAA GCAAGGATTC CCTTCGTGGC 3851 CCCTTCTCTG CTGCCTCTCA GAATCCAGAC GCTAAGGAAA ATCCCTAAGC 3901 AGAGATTTTC TGTTGGATGC TAAAAGCAAG GAATAAAAGT TGAAAATTTG 3951 GAAAATGTCT CAACACCGTC ACCAGCGCCA CTCGAGAGTC ATTTCTAGTT 4001 CACCAGTTGA CACTACATCG GTGGGATTTT GCCCAACATT CAAGAAATTT 4051 AAGTAAATAT TATCTATCTC CATTGCCTGT TAAGAAATGT GCTAGTAGAA 4101 GTGTGAGGGC AGGGTGTCAG TGTTCTCTCA GCCTCTTCCC TCAGATACTC 4151 GTCTGCTTAC CAAAATAAGT TGCATGTCCT TGACAATCTG GTTTCTATGA 4201 TTGGTGAGGC TGGCATGCTA TTACCTTTAT GTGCCCTGTA GACTTGAATG 4251 ACCAGTTTGA CCAGTTTGAC TGTTAGATAA TCAGAAGGCT TTTCTCTTTT 4301 TTTATAATAG ACCCCATCTC AAATCAGATA ATGAAAATTA CATATCTTGA 4351 TATATTAGAA AAGTATATAC ATTCTGGCTG GGCACGGTGG CTCACGCCTG 4401 TAATCCCTGC ACTTTGAGAG GCTGGGGCGG ATCACTTGAG GTCAGGAGTT 4451 TGAGACCGGC CTGGCCAGCG TGGCGAAACC CCATCTCTAC TAAAAATACA 4501 CAGATTAGCC CGGAGTGATG GTGTGCACCT GTTGTCCCAG CTACTCAGGA 4551 TGCTGAGGCA GGAGAATCCC TTTAACCTGG GGGGCGAAGG TTGCAGTGAG 4601 CCAGGATTGC ACCACTGCAC TCCAGCCTGG GTGACGGAAC GGGACTCTGT 4651 CTCAGAAAAA AAAAAAAAGA AGAGGAAAAA GAAAAATATA TATTCTATAT 4701 TTTTTTAACT TATGAGAATG TGTTCATTTC ATTTGTAACA TATAATGGGA 4751 AACAGTAATA CGTACTCTGA GAAAAATTGC AAAGCACAGA TAAATGGAAA 4801 TAAACAGGAA AAAGAATCAC CTATAACCTC ACCATCCATA GACAGACACT 4851 GTTAAAATTT TGGCATATTT CCTGCTGATT TTTTCTACTG CTGATTTTTG 4901 CACAGGTGAG ATAATTTTGA ACAGAGAATT TTGTATCTTT GGTTTTTGTG 4951 TTTCGCTGCA CACAAAAACA AAAGATATAA AAATGGATCA TAAACATTTT 5001 TCTAAATCCT GAAAAGTGCA TAGACATATT TTAGTGCCTG TATTTCACAA 5051 GATGGACATA CCATAATTTA CTTACACAGT CCTTTTTGTT AGATGTTTAA 5101 GTTGTTTTCA AGCTTCTCAG TGCTGGAAAA AATACTGAGA TAGACATGTT 5151 TAGTTGAAGT TATTTCATTT CAGGTTATAT TATCTTGGGT CAGAGAATGA 5201 ATGGTTCTCA GGCTTTTCAA AAGAGCTGGT CAGTTTTTAT GCCTCTGGCA 5251 GTTTTTGAGA GTGCTCAATC ATACTACACT GTTGCCAGCA TTAGATCTTA 5301 TCACATTTAA GTCATTGCTA ATTTTATAAA CAAAAACAAT GGTTTTACTT 5351 TGCATCTCCC TGATTGGTGT TGCTGTAGAA CATATTTGGA GAAGTTTGTT 5401 TGTCTTTGGT GTTTATTCCA TGAATAGATT GTGTGCCCAT TTTCTCTTGG 5451 GGTATTCAGT TTTTTATTAC TGATGTGAGC ATGTGTATGG GTGATTATTT 5501 GATGATTATC AGTTTTGCTT AGTAGACTGG CAATATTTAG TCTTGCTGTC 5551 ACTGTGTTCC CAGTGCCAAC TAGATTGCTT GATATGTAGT TGCCACTCAA 5601 TAAAGATTTG TTGAGTCAAT GAAAAAAAAA AAAAAAAAAA A BLAST Results Entry AC004764 from database EMBL: Homo sapiens chromosome 5, PI clone 255g5 (LBNL H61), complete sequence. Score = 11057, P = 0.0e+00, identities = 2217/2224 Bp 428-5625 of cDNA == Bp 2912-8107 of AC004764 Entry HSAC1555 from database EMBL: Homo sapiens (subclone l_d8 from BAC H75) DNA sequence, complete sequence . Score = 575, P = 5.1e-30, identities = 115/115 Bp -240- 430 of cDNA == HSAC1555 splice pattern Medlme entries 93186787: Phenylalanine hydroxylase-stimulating protem/pterm-4 alpha-carbinolamine dehydratase from rat and human liver. Purification, characterization, and complete ammo acid sequence . 93101632: Identity of 4a-carbιnolamιne dehydratase, a component of the phenylalanine hydroxylation system, and DCoH, a transregulator of homeodomain proteins. 95242099: Crystal structure of DCoH, a bifunctional, protein-binding transcriptional coactivator Peptide information for frame 3 ORF from 21 bp to 410 bp; peptide length: 130 Category: strong similarity to known protein 1 MAAVLGALGA TRRLLAALRG QSLGLAAMSS GTHRLIAEER NQAILDLKAA 51 GWSELSERDA IYKEFSFHNF NQAFGFMSRV ALQAEKMNHH PEWFNVYNKV 101 QITLTSHDCG ELTKKDVKLA KFIEKAAASV BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfkd2_46kl9, frame 3 No Alert BLASTP hits found Pedant information for DKFZphfkd2_46kl9, frame 3 Report for DKFZphfkd2_46kl9.3 [LENGTH] 130 [MW] 14377.56 [pi] 9.17 [HOMOL] PIR:A47189 pterιn-4-alpha-carbmolamιne dehydratase (EC 4.2.1.96) - rat 4e-34 [FUNCAT] 01.07.99 other vitamin, cofactor, and prosthetic group activities [S. cerevisiae, YHL018w] 5e-04 [SCOP] dldchg_ 4.38.1.1.1 Pterιn-4a-carbmolamιne dehydratas 4e-50 [EC] 4.2.1.96 Tetrahydrobiopterin dehydratase 6e-34 [PIRKW] nucleus 6e-34 [PIRKW] carbon-oxygen lyase 6e-34 [PIRKW] homotetramer 6e-34 [PIRKW] hydro-lyase 6e-34 [PIRKW] cytosol 6e-34 [PIRKW] acetylated amino end 6e-34 [PIRKW] homodimer 6e-34 [SUPFAM] pterιn-4-alpha-carbιnolamme dehydratase 6e-34 [PROSITE] MYRISTYL 2 [PROSITE] CK2_PHOSPHO_ΞITE 3 [PROSITE] PKC_PHOSPHO_SITE 4 [KW] Alpha_Beta [KW] 3D [KW] LOW COMPLEXITY 14.62 % SEQ MAAVLGALGATRRLLAALRGQSLGLAAMSSGTHRLIAEERNQAILDLKAAGWSELSERDA SEG . xxxxxxxxxxxxxxxxxxx ldchB CCCCHHHHHHHHHHHHHHCCEEECCCCE SEQ IYKEFSFHNFNQAFGFMSRVALQAEKMNHHPEWFNVYNKVQITLTSHDCGELTKKDVKLA SEG ldchB EEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEETTTEEEEEECBTTTTBTCCHHHHHH SEQ KFIEKAAASV SEG ldchB HHHHHHHHHH Prosite for DKFZphfkd2_46kl9.3 PS00005 11->14 PKC_PHOSPHO_SITE PDOC00005 PS00005 32->35 PKC_PHOSPHO_SITE PDOC00005 PS00005 56->59 PKC_PHOSPHO_SITE PDOC00005 PS00005 113->116 PKC_PHOSPHO_SITE PDOC00005 PS00006 56->60 CK2_PHOSPHO_SITE PDOC00006 PS00006 105->109 CK2_PHOSPHO_ΞITE PDOC00006 PS00006 113->117 CK2_PHOSPHO_SITE PDOC00006 PS00008 6->12 MYRISTYL PDOC00008 PS00008 20->26 MYRISTYL PDOC00008 (No Pfam data available for DKFZphfkd2_46kl9.3) DKFZph kd2 46m4 group: signal transduction DKFZphfkd2_46m4.3 encodes a novel 198 amino acid putative GTP-binding protein related to the SAR-1 family of Ras superfamily members. SARI proteins are involved in vesicular transport between the endoplasmic reticulum and the Golgi apparatus . The new protein can find clinical application in modulating the transport of vesicles to the Golgi Apparatus, thus enabling post-translational modifications of the vesicles contents. Blocking of the molecule is expected to result modulation/blocking of secretory pathways. nearly identical to mouse GTP-bindmg protein complete cDNA, complete eds, EST hits Sequenced by MediGenomix Locus: /map="438.9 cR from top of ChrlO linkage group" Insert length: 2996 bp Poly A stretch at pos. 2969, polyadenylation signal at pos. 2958 1 ACATCCGGCG AGTAGCTGGC GGTCCCGGGT GCTGCTGGTT AGTGTGCTCT 51 GAGGGAGGGT CCGAGCCAGC CGCTGTTTTG CCGGAGGAGC CCCTCAGGCC 101 GTAGTAAGCA TTAATAATGT CTTTCATCTT TGAGTGGATC TACAATGGCT 151 TCAGCAGTGT GCTCCAGTTC CTAGGACTGT ACAAGAAATC TGGAAAACTT 201 GTATTCTTAG GTTTGGATAA TGCAGGCAAA ACCACTCTTC TTCACATGCT 251 CAAAGATGAC AGATTGGGCC AACATGTTCC AACACTACAT CCGACATCAG 301 AAGAGCTAAC AATTGCTGGA ATGACCTTTA CAACTTTTGA TCTTGGTGGG 351 CACGAGCAAG CACGTCGCGT TTGGAAAAAT TATCTCCCAG CAATTAATGG 401 GATTGTCTTT CTGGTGGACT GTGCAGATCA TTCTCGCCTC GTGGAATCCA 451 AAGTTGAGCT TAATGCTTTA ATGACTGATG AAACAATATC CAATGTGCCA 501 ATCCTTATCT TGGGTAACAA AATTGACAGA ACAGATGCAA TCAGTGAAGA 551 AAAACTCCGT GAGATATTTG GGCTTTATGG ACAGACCACA GGAAAGGGGA 601 ATGTGACCCT GAAGGAGCTG AATGCTCGCC CCATGGAAGT GTTCATGTGC 651 AGTGTGCTCA AGAGGCAAGG TTACGGCGAG GGTTTCCGCT GGCTCTCCCA 701 GTATATTGAC TGATGTTTGG ACGGTGAAAA TAAAAGAGTT TTACTTCTCT 751 GGACTGATCC TATTCACAGC TTCCTCATGA ACTTTTCTAA TAGAACAAGG 801 ATAGCTCTCC AACCATGTCT GGCGTTGAGA AGCCAAGAGT CTCTGTCAAC 851 TCTCTCATTG CCCAGTGGTG ACATGTGCTC TTCTCCACAC TGTTGGGAGG 901 TAATGCTGCC CCACGTGCTG GTGCAGGTCA GTATCCTGGG ACTTGGAAGC 951 TGGCAGGATT TGCCGGGTAA AGCTGTATGC CATCATGGGG CACCTGAAAA 1001 GAAAAACACG TCTCACCACT GTGGTTGATT CAAAAGAAAG TGATTCTATT 1051 TTTTAAAGAA AGCGTTGTTA ATGTAATTGG TATCCCTCCT AACTTTTTGA 1101 GTTCACAATT TACTTGGTCC AGAGTTTTCT ATTCTTTTTT TTTTTTTAAA 1151 CTAATGAATG ACATTTAGAT ACTTCATAAA ATTATGAACA GATATGGAGG 1201 CCAGAGCTCA TTTGGGTAAA CTTACTCCTG CTGAGTTAGC AGGTTGGTGA 1251 GAGAAGCTCC CCTGAGCTCA CCTGTCTCTC TGACTGCCTT GGAGTAGGTG 1301 GCATAACCTT GTGCACAGAG AACTAGAAAA GGGGCAGAAC CCCGGCCTTG 1351 CAGTTGTGGC AGGTTTCCAC TGTGGTAAGC TAGGTTCATT CCTCATCAAG 1401 GAATGTGTAG CAGATTGTTC ACTGTGGAGG AGGTAATTAT AGAATGGGTT 1451 ATTGTTGTTA TTCTTACTCA TGAAGTTACA GATTTTAGCC AGTCTTTGCT 1501 TTTATACTTT TGTGAAATTT AATTTCTCTC TATAGCACCT TCCTTTTTCG 1551 TTTTCAGTTA TCAAAAGTGA CTTTGACCTC ATAAGAGAGT TGAGAACATC 1601 TCTCGTGTCA CATACTGCAG GTGCATCAGT TACTTTTGCA CAGATTCTAG 1651 GGGGACATTT TTCTGAATAG GAAGACAGGA CAAAGTTAAC AGCTTAAGGG 1701 CTCTTAATTC TGTGAGTTGA GGACTTAAAA GTATTGTAGC ATTTGTTTGG 1751 ATCCATGAAA AATGTATTCA GTGGGCTTTA AAATTTCCAT TTGCAGAATT 1801 TGGTCTCTCA GGCTGTTTGG GAGCTCTTTT TTTTACATTT TTTCTCCTTT 1851 GACACCTATT TTATTGGTGT TTAAAGTAAA GGTTAACATC TGTAGCTTTT 1901 CCAGGTTTTT TTTTTTTTTT TTGATATGAA ATTGTCTTTC TCCATTGCAG 1951 AAATAAGCTA GGGAAACACT AACCCAAAAA CTTTCTGTAG AGCTGTTCCT 2001 TTGGAGGCAG CATCACTTAT TGGCAGTAAA GACTCAGTAT AAAAGCACCA 2051 GCATCCCTAC TTGGGTGATG GGGATTAATT TTATAGCATT CCATTTTCCT 2101 AGTGCCACAT GTGAAATTGG ATTTTGATGA TCTTAATCTA TATTCTACCC 2151 TTATAATAAA AGATCAAAAG ATATATCTCC TATGAACAGA TTGGAGATAG 2201 GAGATGAAAA GTTGGGAGGA TGCCTTTATT CTAATGTGAG GGTAGGGAAA 2251 ATGTGGATAA CATTACTGGG GTGAAGGAGG CATTGTTCTT TAGTTGGAGT 2301 TCTCATTTTT ATTCTCCAGT ACTGACTTGT GGGGAAAGCA TACTTTTTCA 2351 CTGCCAGGTA CTGAATGCAG AGGCTCAGTG AAGTATATAT GTGGGAAGTG 2401 CATGCATTTC GTTTATTAGC AAACATAGCT GGATTAAGAC GAAGTTGTTG 2451 GTTTGGAAAG GGGTTAAAGC CTTAAGTGAA CAAATCTAGC TAACAGTGAA 2501 TGAACTAGGT AATATAACTT GCATATTTTT AATTTCCTTT GGTTAAAGGT 2551 CCCCCATACT TCTCTGTTCG GAGACATGAG AAGTATGATT ACTTCAGTGT 2601 TAGTTTTCTT AATTTTTTTT TTCCCCTATT TGTCCCTTGT CACTTTGTTG 2651 CAAGCTAGAA ATCTGTGGGT TATACATAGG GCAGCTCTTT GCGAAAGTGG 2701 TTTATTCCAC TGGAGAAAGG GGATTGAAAA TCAGTTAGAA CCAATGTATT 2751 TCTTGCCCCA CGGAACACTA TTCCTATAAG ATAGCTGAAA GAAGCTGCTG 2801 TGAGGAGCTC AGCTCCAACA CAGGATCAGC ACCTTGTATA GGAATTCCCA 2851 TGAATTATGA CTTCTCATTC TGTTTTATCA GAGTGCATAT ATGTCCTACT 2901 TCAGGAAAAG TAAAACAGTC ATTTACGAAA GAAAGTCAAT CTGTATCCTA 2951 AGCATTTTAA TAAAAAGTTA AAACAAAAAA AAAAAAAAAA AAAAAA BLAST Results Entry HS679348 from database EMBL: human STS WI-16722. Length = 265 Minus Strand HSPs: Score = 1242 (186.4 bits), Expect = 2.8e-50, P = 2.8e-50 Identities = 260/265 (98%) Medline entries 94085558: Molecular analysis of SARl-related cDNAs from a mouse pituitary cell line. Peptide information for frame 3 ORF from 117 bp to 710 bp; peptide length: 196 Category: strong similarity to known protein 1 MSFIFEWIYN GFSSVLQFLG LYKKSGKLVF LGLDNAGKTT LLHMLKDDRL 51 GQHVPTLHPT SEELTIAGMT FTTFDLGGHE QARRVWKNYL PAINGIVFLV 101 DCADHSRLVE SKVELNALMT DETISNVPIL ILGNKIDRTD AISEEKLREI 151 FGLYGQTTGK GNVTLKELNA RPMEVFMCSV LKRQGYGEGF RWLSQYID BLASTP hits Entry S39543 from database PIR: GTP-binding protein - mouse Length = 198 Score = 1029 (362.2 bits), Expect = 5.1e-104, P = 5.1e-104 Identities = 197/198 (99%), Positives = 198/198 (100%) Entry SARA_MOUSE from database SWISSPROT: GTP-BINDING PROTEIN SARA. Length = 198 Score = 1012 (356.2 bits), Expect = 3.2e-102, P = 3.2e-102 Identities = 195/198 (98%), Positives = 196/198 (98%) Entry CEZK180_4 from database TREMBL: gene: "ZK180.4"; Caenorhabditis elegans cosmid ZK180. Length = 193 Score = 679 (239.0 bits), Expect = 6.3e-67, P = 6.3e-67 Identities = 125/197 (63%), Positives = 161/197 (81%) Alert BLASTP hits for DKFZphfkd2_46m4, frame 3 No Alert BLASTP hits found Pedant information for DKFZphf d2_46m4, frame 3 Report for DKFZphfkd2_46m4.3 [LENGTH] 198 [MW] 22367.00 [pi] 6.21 [HOMOL] PIR:S39543 GTP-binding protein - mouse le-112 [FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YPL218w] le-58 [FUNCAT] 30.09 organization of intracellular transport vesicles [S. cerevisiae, YPL218w] le-58 [FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YOR094w] 2e-23 [FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylation, farnesylation and processing) [S. cerevisiae, YPL051w] 4e-22 [FUNCAT] 30.08 organization of golgi [S. cerevisiae, YDL192w] 3e-20 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YBR164c] 3e-19 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YMR138w] 2e-09 [FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YMR138w] 2e-09 [FUNCAT] 98 classification not yet clear-cut [S. cerevisiae, YHR168w] 7e-05 [FUNCAT] 30.02 organization of plasma membrane [S. cerevisiae, YHR005c] le-04 [FUNCAT] 30.07 organization of endoplasmatic reticulum [S. cerevisiae, YKL154w] le-04 [FUNCAT] 03.07 pheromone response, mating-type determination, sex-specific proteins [S cerevisiae, YHR005c] le-04 [FUNCAT] 10.05.07 g-protems [S. cerevisiae, YHR005c] le-04 [FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YKL154w] le-04 [FUNCAT] 08.19 cellular import [S. cerevisiae, YMLOOlw] 3e-04 [BLOCKS] BL00395A Alanine racemase pyridoxal-phosphate attachment site proteins [BLOCKS] BL01019B ADP-ribosylation factors family proteins [BLOCKS] BL01019A ADP-ribosylation factors family proteins [BLOCKS] BL01020D SARI family proteins [BLOCKS] BL01020C SARI family proteins [BLOCKS] BL01020B SARI family proteins [BLOCKS] BL01020A SARI family proteins [SCOP] dlplj_ 3.25.1.3.1 cH-p21 Ras protein [human (Homo sapiens) 7e-36 [SCOP] dlguaa_ 3.25.1.3.10 RaplA [Human (Homo sapiens) 8e-40 [SCOP] dlrrf 3.25.1.3.5 ADP-ribosylation factor 1 (ARFl) [rat (Rattu 2e-55 [SCOP] dlhurb_ 3.25.1.3.4 ADP-ribosylation factor 1 (ARFl) [human (Hom le-58 [SCOP] dlgota2 3.25.1.3.3 (1-54,171-326) Transduem (alpha subunit) [ra 2e-33 [SCOP] dltadb2 3.25.1.3.2 (1-30,152-316) Transduem (alpha subunit 6e-36 [PIRKW] glycoprotein 4e-19 [PIRKW] monomer le-16 [PIRKW] P-loop 3e-64 [PIRKW] lipoprotein 4e-19 [PIRKW] GTP binding 3e-64 [SUPFAM] ADP-ribosylation factor 5e-22 [PROSITE] ATP_GTP_A 1 [PROSITE] MYRISTYL 3 [PROSITE] SARI 1 [PROSITE] CK2_PHOSPHO_SITE 4 [PROSITE] PKC_PHOSPHO_SITE 3 [PROSITE] ASNJ3LYCOSYLATION 1 [PFAM] ADP-ribosylation factors Arf family) (contains ATP/GTP binding P-loop) [KW] Alpha_Beta [KW] 3D SEQ MSFIFEWIYNGFSSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPT lhurA TTTTTCCCCEEEEEETTTTCHHHHHHHHCCCCEEEEEEETTEE SEQ ΞEELTIAGMTFTTFDLGGHEQARRVWKNYLPAINGIVFLVDCADHSRLVESKVELNALMT lhurA EEEEEETTEEEEEEETTTTTTTCCCHHHHHHCEEEEEEEEETTTTTHHHHHHHHHHHHHH SEQ DETISNVPILILGNKIDRTDAISEEKLREIFGLYGQTTGKGNVTLKELNARPMEVFMCSV lhurA TTTTTTTEEEEEEETTTTTTTCCHHHHHHHHCGG SEQ LKRQGYGEGFRWLΞQYID lhurA Prosite for DKFZphfkd2_46m4.3 PS00001 162->166 ASN_GLYCOSYLATION PDOC00001 PS00005 25->28 PKC_PHOSPHO_SITE PDOC00005 PS00005 158->161 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 164->167 PKC_PHOSPHO_SITE PDOC00005 PS00006 60->64 CK2_PHOSPHO_SITE PDOC00006 PS00006 72->76 CK2_PHOΞPHO_SITE PDOC00006 PS00006 111->115 CK2_PHOSPHO_SITE PDOC00006 PS00006 164->168 CK2_PHOSPHO_SITE PDOC00006 PS00008 32->38 MYRISTYL PDOC00008 PS00008 68->74 MYRISTYL PDOC00008 PS00008 155->161 MYRISTYL PDOC00008 PS00017 32->40 A P_GTP_A PDOC00017 PS01020 171->197 SARI PDOC00782 Pfam for DKFZphfkd2_46m4.3 HMM_NAME ADP-ribosylation factors (Arf family) (contains ATP/GTP binding P-loop) HMM *GMgWfsIFrkMWGlWNKEMRILMLGLDNAGKTTILYMLKlgEIVTTIPT ++ FS++++++GL++K++++++LGLDNAGKTT+L+MLK++++ +++PT Query 9 -YNGFSSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPT 56 HMM IGFNVETVeYKNIKFNVWDVGGQdsIRPYWRHYYpNTDGIIWVVDSaDRD +++++E++++ +++F+++D+GG++++R++W++Y P+++GI+++VD+AD++ Query 57 LHPTSEELTIAGMTFTTFDLGGHEQARRVWKNYLPAINGIVFLVDCADHS 106 HMM RMeEaKqELHaMLNEEELrDAPlLIFANKQDLPgAMSesEIREaLGLHel R+ E+K+EL+A++++E ++++P+LI++NK+D+ +A+SE+++RE+ GL+ + Query 107 RLVESKVELNALMTDETISNVPILILGNKIDRTDAISEEKLREIFGLYGQ 156 HMM RCn RPWYIQMCCAVtGEGLYEGMDWLSNYInkRkK* +++ RP++++MC++++++G++EG++WLS+YI Query 157 TTGKGNVTLKELNARPMEVFMCSVLKRQGYGEGFRWLSQYI 197 DKFZphfkd2_47a4 group: transcription factor DKFZphfkd2_47a4.1 encodes a novel 280 ammo acid protein with similarity to zinc fmger proteins. The new protein is a putative transcription factor with one C2H2 zinc fingers. The new protein can find application m modulating/blocking the expression of genes controlled by this transcription factor. similarity to C. elegans F46B6.7 potential frame shift at 1092, will be checked see BLASTX Sequenced by MediGenomix Locus: map="7q31" Insert length: 1756 bp Poly A stretch at pos. 1737, no polyadenylation signal found 1 CCCTTTTCTT TTCTGCCGGG TAATGGCTGC TTCCAAGACC CAGGGGGCTG 51 TCGCCCGAAT GCAGGAAGAC CGTGATGGGA GCTGCAGCAC AGTCGGGGGT 101 GTAGGTTATG GGGTAAGGAT TGTATCCTGG AGCCGCTTTC CCTGCCAGAA 151 AGTCCAGGTG GCACCACCAC TTTAGAAGGT TCTCCATCTG TGCCTTGTAT 201 TTTCTGTGAA GAACATTTTC CTGTGGCTGA ACAAGACAAA CTTCTGAAGC 251 ACATGATTAT TGAGCATAAG ATTGTCATAG CTGATGTCAA GTTGGTTGCT 301 GATTTCCAAA GGTACATTTT ATATTGGAGG AAAAGGTTCA CTGAACAGCC 351 CATCACAGAT TTTTGTAGTG TAATAAGAAT TAATTCCACT GCTCCATTTG 401 AAGAACAAGA GAATTATTTT TTGTTATGTG ACGTTTTACC AGAAGATAGA 451 ATTCTTAGAG AAGAGCTTCA GAAACAGAGA CTGAGAGAAA TTCTGGAACA 501 ACAGCAGCAA GAACGAAATG ATAACAATTT TCATGGCGTT TGTATGTTTT 551 GCAATGAAGA ATTCCTTGGA AACAGATCTG TTATTTTGAA CCACATGGCC 601 AGAGAACATG CTTTCAACAT TGGATTGCCA GACAACATTG TAAACTGCAA 651 TGAATTTTTG TGTACATTAC AGAAAAAGCT TGACAATTTG CAGTGCTTGT 701 ACTGTGAGAA GACCTTCAGG GGCAAAAATA CACTTAAAGA TCACATGAGG 751 AAAAAACAGC ATCGTAAGAT TAATCCTAAG AACAGAGAAT ATGACAGATT 801 TTATGTCATC AATTATTTGG AACTTGGAAA ATCGTGGGAG GAAGTTCAGT 851 TGGAAGATGA TCGGGAGTTG CTGGACCATC AGGAAGATGA CTGGTCTGAT 901 TGGGAAGAAC ACCCTGCCTC TGCAGTCTGC TTATTTTGTG AAAAGCAAGC 951 AGAAACAATT GAGAAGTTGT ATGTCCACAT GGAGGATGCA CACGAATTTG 1001 ATCTTCTCAA AATAAAGTCA GAACTTGGAT TAAATTTCTA TCAGCAAGTG 1051 AAACTGGTCA ATTTTATTCG GAGGCAAGTT CACCAATGCA GATGATGGCT 1101 GCCATGTGAA GTTCAAATCC AAAGCAGACT TAAGAACTCA CATGGAAGAA 1151 ACTAAACACA CTTCGCTGCT CCCCGATAGA AAGACGTGGG ATCAACTGGA 1201 GTATTATTTT CCAACCTATG AAAATGACAC TCTCCTGTGT ACACTATCTG 1251 ACAGTGAAAG TGACCTGACA GCTCAGGAAC AAAATGAAAA TGTTCCCATC 1301 ATCAGTGAAG ATACATCTAA ACTGTATGCT TTGAAACAAA GCAGTATTTT 1351 GAACCAGTTG CTACTATAAG AGTACTTGAA AACCTAGAAG AAACTACCAC 1401 AGAAGCAATT TTTCATGTTT TTCTCCTATG AGACAGATAT GAAAGAACAA 1451 TTTAAATTTG AACATCAACA AAAGATTGGT CCTTGGTGAA ATAAACTTTT 1501 CAAAAATGAA TGTTCTTTTC AAAAAATAAA GTAGAAAAAT GCACTTACTA 1551 AGAACATGAA AAAAAAATGA AGTAGGAAAA TAAGATGAAG ACTTTGTATT 1601 TTGGCTGTAA AGTTTTATTG TGTGATCATC TTAAATTATC TCACTTCATT 1651 AAACTCATAA TTATATA AG AAGTA ATGT CAATTACAAA GAAATGAAAT 1701 GTTCAAATTA TTTATAAACC TGATTTTTCA ATCAGCGAAA AAAAAAAAAA 1751 AAAAAA BLAST Results Entry AC004112 from database EMBL: Homo sapiens BAC clone RG313E03 from 7q31, complete sequence. Score = 2660, P = 3.0e-241, identities = 534/535 > 10 exons Entry AC004111 from database EMBL: Homo sapiens BAC clone RG103H13 from 7q31, complete sequence. Score = 598, P = 5.8e-17, identities = 128/137 1 exon Medline entries No Medline entry Peptide information for frame 1 ORF from 253 bp to 1092 bp; peptide length: 280 Category: similarity to unknown protein 1 MIIEHKIVIA DVKLVADFQR YILYWRKRFT EQPITDFCSV IRINSTAPFE 51 EQENYFLLCD VLPEDRILRE ELQKQRLREI LEQQQQERND NNFHGVCMFC 101 NEEFLGNRSV ILNHMAREHA FNIGLPDNIV NCNEFLCTLQ KKLDNLQCLY 151 CEKTFRGKNT LKDHMRKKQH RKINPKNREY DRFYVINYLE LGKSWEEVQL 201 EDDRELLDHQ EDDWSDWEEH PASAVCLFCE KQAETIEKLY VHMEDAHEFD 251 LLKIKSELGL NFYQQVKLVN FIRRQVHQCR BLASTP hits Entry CEF46B6_6 from database TREMBLNEW: product: "F46B6.7"; Caenorhabditis elegans cosmid F46B6 >TREMBL:CEF46B6_6 product: "F46B6.7"; Caenorhabditis elegans cosmid F46B6 Score = 630, P = l.le-61, identities = 123/289, positives = 183/289 Entry AF059531_1 from database TREMBLNEW: gene: "PRMT3"; product: "protein arginine N-methyltransferase 3"; Homo sapiens protein arginine N-methyltransferase 3 (PRMT3) mRNA, partial eds. >TREMBL:AF059531_1 gene: "PRMT3"; product: "protein arginine N-methyltransferase 3"; Homo sapiens protein arginine N-methyltransferase 3 (PRMT3) mRNA, partial eds. Score = 120, P = 1.5e-04, identities = 23/78, positives = 42/78 Entry YB9M_YEAST from database SWISSPROT: 34.7 KD PROTEIN IN SHM1-MRPL37 INTERGENIC REGION. Score = 112, P = 4.6e-04, identities = 43/165, positives = 71/165 Alert BLASTP hits for DKFZphfkd2_47a4, frame 1 No Alert BLASTP hits found Pedant information for DKFZphfkd2_47a4, frame 1 Report for DKFZphfkd2_47a4.1 [LENGTH] 280 [MW] 33921.94 [pi] 5.63 [HOMOL] TREMBL :CEF46B6_5 gene: "F46B6.7"; Caenorhabditis elegans cosmid F46B6 le-56 [BLOCKS] BL01032B Protein phosphatase 2C proteins [BLOCKS] BL00028 Zinc finger, C2H2 type, domain proteins [PROSITE] MYRISTYL 1 [PROSITE] ZINC_FINGER_C2H2 1 [PROSITE] CAMP_PHOSPHO_SITE 1 [PROSITE] CK2_PHOSPHO_SITE 3 [PROSITE] TYR_PHOSPHO_SI E 2 [PROSITE] PKC_PHOSPHO_SITE 2 [PROSITE] ASN_GLYCOSYLATION 2 [PFAM] Z nc finger, C2H2 type [KW] Alpha_Beta [KW] LOW COMPLEXITY 8.21 % SEQ MIIEHKIVIADVKLVADFQRYILYWRKRFTEQPITDFCSVIRINSTAPFEEQENYFLLCD SEG PRD cccccceeehhhhhhhhhhhhhhhhhhhhhhhcccceeeeeeccccccchhhhheeeecc SEQ VLPEDRILREELQKQRLREILEQQQQERNDNNFHGVCMFCNEEFLGNRSVILNHMAREHA SEG xxxxxxxxxxxxxxxxxxxxxxx PRD ccccchhhhhhhhhhhhhhhhhhhhhhhhcccceeeeeeccccccccceeeehhhhhhhh SEQ FNIGLPDNIVNCNEFLCTLQKKLDNLQCLYCEKTFRGKNTLKDHMRKKQHRKINPKNREY SEG PRD hcccccccccchhhhhhhhhhhhhhhhheeecccccccchhhhhhhhhhhcccccccccc SEQ DRFYVINYLELGKSWEEVQLEDDRELLDHQEDDWSDWEEHPASAVCLFCEKQAETIEKLY SEG PRD ceeeeeeeeccccchhhhhhhhcchhhhhhcccccccccccccccchhhhhhhhhhhhhh SEQ VHMEDAHEFDLLKIKSELGLNFYQQVKLVNFIRRQVHQCR SEG PRD hhhhhhhhhhhhhhhhhhcchhhhhhhhhhhhhhhhcccc Prosite for DKFZphfkd2_47a4.1 PS00001 44->48 ASN_GLYCOSYLATION PDOC00001 PS00001 107->111 ASN_GLYCOSYLATION PDOC00001 PS00004 27->31 CAMP_PHOSPHO_SITE PDOC00004 PS00005 154->157 PKC_PHOSPHO_SITE PDOC00005 PS00005 160->163 PKC_PHOSPHO_SITE PDOC00005 PS00006 160->164 CK2_PHOSPHO_SITE PDOC00006 PS00006 194->198 CK2_PHOSPHO_SITE PDOC00006 PS00006 215->219 CK2_PHOSPHO_SITE PDOC00006 PS00007 178->185 TYR_PHOSPHO_SITE PDOC00007 PS00007 13->22 TYR_PHOSPHO_SITE PDOC00007 PS00008 124->130 MYRISTYL PDOC00008 PS00028 148-M71 ZINC FINGER C2H2 PDOC00028 Pfam for DKFZphfkd2_47a4.1 HMM_NAME Zmc finger, C2H2 type HMM *CpwPDCgKtFrrwsNLrRHMR..T.H* C + C+KTFR + +L+ HMR H Query 148 CLY--CEKTFRGKNTLKDHMRKK-QH 170 DKFZphfkd2_4b6 group: kidney derived DKFZphfkd2_4b6 encodes a novel 133 amino acid protein with similarity to Homo sapiens clone 25003 partial CDS. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of kidney-specific genes . similarity to Homo sapiens clone 25003 complete cDNA, complete eds, few EST hits Sequenced by GBF Locus : unknown Insert length: 1936 bp Poly A stretch at pos. 1916, polyadenylation signal at pos. 1890 1 GGGAGACTTG CAATGAAGTT AGAATGAACA GGAGGAGTCT GCAGCTTTTC 51 AGTGCCTGGG ATAACTATAG TTTAAAGATC ATTGTGTAAA ATAGGATTTT 101 TAGTCAGCAT GCATTGTTTT AAACCGACTA ACTGATAGCC TAAAACTTTA 151 TTTTTGCATT TTGCCAATCC TTGGAGTTTT GTTTTGCAGA ATTAAGAAAA 201 AAATGAATGT ATGATCATCT GAAAAGGGCT TTCTCTCAAT CCCACTTCAT 251 GGCATGACCT CTGCTGGATC ATTAGTTCTA GCCAGAGAAG TAGCAAAGGA 301 ACATGACGTC TGAGACCTCC CTTCCCTCAT CAGTGGGGCT GACTGAGCTG 351 GGGGCTTGAA GCCGGAGGTA ACCTTTCCTG TCGAATGTTT CTTTAGAGAA 401 TGGCAATGGT CTCTGCGATG TCCTGGGTCC TGTATTTGTG GATAAGTGCT 451 TGTGCAATGC TACTCTGCCA TGGATCCCTT CAGCACACTT TCCAGCAGCA 501 TCACCTGCAC AGACCAGAAG GAGGGACGTG TGAAGTGATA GCAGCACACC 551 GATGTTGCAA CAAGAATCGC ATTGAGGAGC GGTCACAAAC AGTAAAGTGT 601 TCCTGTCTAC CTGGAAAAGT GGCTGGAACA ACAAGAAACC GGCCTTCTTG 651 CGTCGATGCC TCCATAGTGA TTTGGAAATG GTGGTGTGAG ATGGAGCCTT 701 GCCTAGAAGG AGAAGAATGT AAGACACTCC CTGACAATTC TGGATGGATG 751 TGCGCAACAG GCAACAAAAT TAAGACCACG AGAATTCACC CAAGAACCTA 801 ACAGAAGCAT TTGTGGTAGT AAAGGAAAAC CAACCCTCTG GAAAATACAT 851 TTTGAGAATC TCAAACATCT CACATATATA CAAGCCAAAT GGATTTCTTA 901 CTTGCACTTT GACTGGCTAC CAGATAATCA CAGTGCGTTT AGTGTGTGTA 951 ACGAAATATC CTACAGTGAG AAGACACAGC GTTTTGGCAT CACCATGGAA 1001 AGTGGGCTTA AAAAAGGGTC TTCTCAGTGA AATTTTTGGG CATCATGAAG 1051 AACGATCAAC TATCTTCTAA TTTGAATCTA TAGTTACTTT GTACCATTTG 1101 AAATATATGT ATATATATAT ATATAATATT TTGAAATATT ATCTATTCTC 1151 TTCAAGAAAT GAACAGTACC ACAGTTTGAG ACGGCTGGTG TACCCCTTTG 1201 AGTTTTGGAT GTTTTGTCTG TTTTGCTTTG TTTTGTTAGT CATTTCTTTT 1251 TCTAACGGCA AGGAAGATAT GTGCCCTTTT GAGAATTCAA GATGGCACTG 1301 ACACGGGAAG GCCAGCTACA GGTGGACTCC TGGAATTTGA GGCATCATAA 1351 TGATACTGAA TCAAGAACTT CCTTCTGCTT CTACCAGATG GCCCAAGGAA 1401 GCACATCGTC CTGTTTTATT GCTTTCTACC CTGTGCAATA TTAGCATGCA 1451 AGCTTGGCTT ACATAGTCAT ACTTTATATT CAATTGATAT ATAATAACCG 1501 TTCTAACCTC TTCCAGGAAA ATATTTTTAG AACTACTAGC TTTTCCACTT 1551 AGAAGAAAAT GAGGATTCTT AAGGGAGCCA CTCCACCATG CTATTAAGAC 1601 TCTGGCAGAG TTATGGGTAG GATATGGATC CCTACATGAA TAAGTCCTGT 1651 AAATACAATG TCTTAAGGCT TTGTATAGCT GTCCTAGACT GCAGAAATGT 1701 CCTCTGATTA AATCCAAAGT CTGGCATCGT TAACTACATA GTGCTGTAGC 1751 AACAAGTCTT ATCATGGCAT CTCTTTCTAT GTTTGGTTTG CTTTTTCCAA 1801 GAGTATTCAG GTCTCCTCTT GTGAGATAGG AAGGCCATGA AAACAATTAG 1851 ATTTCAAGAT GATCTATGTG ACCAAATGTT GGACAGCCCT ATTAAAGTGG 1901 TAAACAACTT CTTTCTAAAA AAAAAAAAAA AAAAAA BLAST Results o BLAST result Medl e entries o Medlme entry Peptide information for frame 1 ORF from 400 bp to 798 bp; peptide length: 133 Category: similarity to unknown protein Classification: no clue 1 MAMVSAMSWV LYLWISACAM LLCHGSLQHT FQQHHLHRPE GGTCEVIAAH 51 RCCNKNRIEE RSQTVKCSCL PGKVAGTTRN RPSCVDASIV IWKWWCEMEP 101 CLEGEECKTL PDNSGWMCAT GNKIKTTRIH PRT BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfkd2_4b6, frame 1 TREMBLNEW:AF131851_1 product: "Unknown"; Homo sapiens clone 25003 mRNA sequence, partial eds., N = 1, Score = 242, P = 1.7e-20 >TREMBLNEW:AF131851_1 product: "Unknown"; Homo sapiens clone 25003 mRNA sequence, partial eds. Length = 165 HSPs: Score = 242 (36.3 bits), Expect = 1.7e-20, P = 1.7e-20 Identities = 44/89 (49%), Positives = 58/89 (65%) Query: 42 GTCEVIAAHRCCNKNRIEERSQTVKCSCLPGKVAGTTRNRPSCVDASIVIWKWWCEMEPC 101 GTCE++ R ++ R QT +C+C G++AGTTR RP+CVDA 1+ K WC+M PC Sbjct: 76 GTCEIVTLDRDSΞQPRRTIARQTARCACRKGQIAGTTRARPACVDARIIKTKQWCDMLPC 135 Query: 102 LEGEECKTLPDNSGWMCAT-GNKIKTTRI 129 LEGE C L + SGW C G +IKTT + Sbjct: 136 LEGEGCDLLINRSGWTCTQPGGRIKTTTV 164 Pedant information for DKFZphf d2_4b6, frame 1 Report for DKFZphf d2_4b6.1 [LENGTH] 133 [MW] 15030.64 [pi] 8.49 [HOMOL] TREMBLNEW:AF131851_1 product: "Unknown"; Homo sapiens clone 25003 mRNA sequence, partial eds. 4e-20 [KW] Alpha_Beta [KW] SIGNAL_PEPTIDE 26 SEQ MAMVSAMSWVLYLWISACAMLLCHGSLQHTFQQHHLHRPEGGTCEVIAAHRCCNKNRIEE PRD ccchhhhhhhhhhhhhhhhhhhhccccchhhhhhhcccccccceeeeeeecccccchhhh SEQ RSQTVKCSCLPGKVAGTTRNRPΞCVDASIVIWKWWCEMEPCLEGEECKTLPDNSGWMCAT PRD hhhhhhccccccccccccccccccceeeeeehhhhhhccccccccceeeecccccceeec SEQ GNKIKTTRIHPRT PRD ccccccccccccc (No Prosite data available for DKFZphfkd2_4b6.1) (No Pfam data available for DKFZphfkd2_4b6.1) DKFZphfkd2_4c8 group: kidney derived DKFZphfkd2_4c8 encodes a novel 153 amino acid protein with partial similarity to huntington's associated protein HAP1. The novel protein contains a leucine zipper involved in protem-protem interaction. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application m studying the expression profile of kidney-specific genes . similarity to KIAA0549 and HAP1 potential frame shift at Bp -1350-1500 will be checked Sequenced by GBF Locus : unknown Insert length: 3182 bp Poly A stretch at pos. 3162, polyadenylation signal at pos. 3135 1 GGGCTTCCCC CATAGAATTT TTCTTTTCAT TGCCCACTTT ACTGTTTTGG 51 CTCCAGACTG TCGTTAAGAA TGTACAGCCT AATTCTGGTG TGTTTCGGGA 101 TATTCTTCTG TCCAGTATTC TGGAAGGGCG GGGAGGCATG GCAGCGTTTT 151 ACTTGACGTT GATGGTGCTG TGAAGTCCAT TCTTTCCTCT GCAAGACTAC 201 TGACTATGCA GAAATTTATC GAAGCGGATT ATTATGAACT AGACTGGTAT 251 TATGAAGAAT GCTCGGATGT TTTATGTGCT GAAAGAGTTG GCCAGATGAC 301 TAAGACATAT AATGACATAG ATGCTGTCAC TCGGCTTCTT GAGGAGAAAG 351 AGCGGGATTT AGAATTGGCC GCTCGCATCG GCCAGTCGTT GTTGAAGAAG 401 AACAAGACCC TAACCGAGAG GAACGAGCTG CTGGAGGAGC AGGTGGAACA 451 CATCAGGGAG GAGGTGTCTC AGCTCCGGCA TGAGCTGTCC ATGAAGGATG 501 AGCTGCTTCA GTTCTACACC AGCGCAGCGG AGGAGAGTGA GCCCGAGTCC 551 GTTTGCTCAA CCCCGTTGAA GAGGAATGAG TCGTCCTCCT CAGTCCAGAA 601 TTACTTTCAT TTGGATTCTC TTCAAAAGAA GCTGAAAGAC CTTGAAGAGG 651 AGAATGTTGT ACTTCGATCC GAGGCCAGCC AGCTGAAGAC AGAGACCATC 701 ACCTATGAGG AGAAGGAGCA GCAGCTGGTC AATGACTGCG TGAAGGAGCT 751 GAGGGATGCC AATGTCCAGA TTGCTAGTAT CTCAGAGGAA CTGGCCAAGA 801 AGACGGAAGA TGCTGCCCGC CAGCAAGAGG AGATCACACA CCTGCTATCG 851 CAAATAGTTG ATTTGCAGAA AAAGGCAAAA GCTTGCGCAG TGGAAAATGA 901 AGAACTTGTC CAGCATCTGG GGGCTGCTAA GGATGCCCAG CGGCAGCTCA 951 CAGCCGAGCT GCGTGAGCTG GAGGACAAGT ACGCAGAGTG CATGGAGATG 1001 CTGCATGAGG CGCAGGAGGA GCTGAAGAAC CTCCGGAACA AAACCATGCC 1051 CAATACCACG TCTCGGCGCT ACCACTCACT GGGCCTGTTT CCCATGGATT 1101 CCTTGGCAGC AGAGATTGAG GGAACGATGC GCAAGGAGCT GCAGTTGGAA 1151 GAGGCCGAGT CTCCAGACAT CACTCACCAG AAGCGTGTCT TTGAGACAGT 1201 AAGAAACATC AACCAGGTTG TCAAGCAGAG ATCTCTGACC CCTTCTCCCA 1251 TGAACATCCC CGGCTCCAAC CAGTCCTCGG CCATGAACTC CCTCCTGTCC 1301 AGCTGCGTCA GCACCCCCCG GTCCAGCTTC TACGGCAGCG ACATAGGCAA 1351 CGTCGTCCTC GACAACAAGA CCAACAGCAT CATTCTGGAA ACAGAGGCAG 1401 CCGACCTGGG AAACGATGAG CGGAGTAAGA AGCCGGGGAC GCCGGGCACC 1451 CCCAGGCTCC CACGACCTGG AGACGGCGCT GAGGCGGCTG TCCCTGCGCC 1501 GGGAGAACTA CCTCTCGGAG AGGAGGTTCT TTGAGGAGGA GCAAGAGAGG 1551 AAGCTCCAGG AGCTGGCGGA GAAGGGCGAG CTGCGCAGCG GCTCCCTCAC 1601 ACCCACTGAG AGCATCATGT CCCTGGGCAC GCACTCCCGC TTCTCCGAGT 1651 TCACCGGCTT CTCTGGCATG TCCTTCAGCA GCCGCTCCTA CCTGCCTGAG 1701 AAGCTCCAGA TCGTGAAGCC GCTGGAAGGT GATCACGCGG GGCCTCGGCC 1751 CCTCTCTGTC CTCCTGGGGG ACTCCCTTTG GTCCCTGATC CACCTGCGGA 1801 AGGCGGGGCA CCTCTGTCAC GCCTACTCCT TTTTCTTCCG CGACAGCCAC 1851 CCGCGCTGCT GGTTTGAGTT CCTCTGAGGG TGGTGCTCAG CCTAGGCCTC 1901 CGTCCCTCCC CTCTGGCTGG CAGGTGTGAC AATGCACACA TAGGCCATGA 1951 AACTCGCCGA GGAAAGACAA GCATGTGCAC TGTGGTCTTC TAGTTCTTTC 2001 CTTTGCCTTT AGAACCTTAG AAATAAAAAC TTTTGTGGCG GTAGAGGCAC 2051 TGCTAACTGA TTCAAAAATT AATTAGGTTT TGCCTGTGGG TGTGAGGAAT 2101 GCAGAAAATT AATGCTTTAG CTTTTCTGCA GTTTTGGTGT CGGGGAGAGG 2151 TTCCAAGCAA ACTCTATTAA ATGGGGATTT TTTTTTCCCC ATAACCACCT 2201 GAATGTGATT TGTGGGCTTA TGTGTTCTGA TTTGAACTTC ATATAGCAAG 2251 GTTGTGGCTT TTGGCAGATG CAGTATGTTC TGAGCGCGGC TCCTAGAGTC 2301 TACAATTTGG AGTCCAGGAA GGGGTGGCTG TGGAGACAAG TGAGTTTTGT 2351 ACCTCCGTAA GCCACCCTTT TTCAGGGTCA GTTCATGTGT TAGTATCAGG 2401 GGCATCTCAG ATGATTAAAC TCATGGGAAA AACTTCCTCC TTCCCTCTCT 2451 CCCTCTTGCC CTCCTGCCTC TTTTTTTTTT TTTTTTTTTT AATTTGGGCA 2501 CTTATAAAAT GTTTTCCCTC TACCTGCTGC TACTCTGCCA AGAGCCACCA 2551 AGTGCTTATA TTTTTCATTT TTTACTCCTT TAGTTTGGAA AGCCATATAC 2601 GTTTGAGAAG GTGTTTTAAA ACTCTGTGTT ACACTTACGA TGCAAAGCCA 2651 AATCAGAACT TCTGTAAGGC AGAACTTTCC CAACTTTAAA AAAATTATTG 2701 TCCCCTCTAG GAGCCTTCTT AGACGTTTTT TCCTAATCAC CCCCCAAAGA 2751 CATTTTAATA CCACATATAT ATTGTTTATG TACTATATGT ATATACATAA 2801 ACAATACATA AGCAATACAT CTGTGGTATT AAAATTAAAA AGAATCCAAT 2851 TATGTTTACC TCAAAAGAAC CTGTTTTTGC TTCTTGGGAG CAATATTGCC 2901 CCTGTGAGAC TGCATGCTAT AAGGTAAGGT TGTGCTTGTT AAAGACCCAA 2951 GACATGACTG GGTTCCACAG TCTCCAAAGG AAGAGGGTGG GCTAGTTTGT 3001 TTTTATTATT ATTTTAAAAT TGTATAATTG GGGTCTTTCT TAGAGTTCAG 3051 AAAAGGTATA GCTTACTCTT TTTTAATTGT TTATTTAGTT GTAAGCTTAG 3101 TGATTGTTTT CTGATCCACA TTGTGTGTGT TCTTCAATAA AATCTTTCAT 3151 TTCTGCAATT TTAAAAAAAA "AAAAAAAAAA AA BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 2 ORF from 206 bp to 1531 bp; peptide length: 442 Category: similarity to known protein Classification: unset Prosite motifs: LEUCINE ZIPPER (139-161) 1 MQKFIEADYY ELDWYYEECS DVLCAERVGQ MTKTYNDIDA VTRLLEEKER 51 DLELAARIGQ SLLKKNKTLT ERNELLEEQV EHIREEVSQL RHELSMKDEL 101 LQFYTSAAEE SEPESVCSTP LKRNESSSSV QNYFHLDSLQ KKLKDLEEEN 151 VVLRSEASQL KTETITYEEK EQQLVNDCVK ELRDANVQIA SISEELAKKT 201 EDAARQQEEI THLLSQIVDL QKKAKACAVE NEELVQHLGA AKDAQRQLTA 251 ELRELEDKYA ECMEMLHEAQ EELKNLRNKT MPNTTSRRYH SLGLFPMDSL 301 AAEIEGTMRK ELQLEEAESP DITHQKRVFE TVRNINQVVK QRSLTPSPMN 351 IPGSNQSSAM NSLLSSCVST PRSSFYGSDI GNVVLDNKTN SIILETEAAD 401 LGNDERSKKP GTPGTPRLPR PGDGAEAAVP APGELPLGEE VL BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfkd2_4c8, frame 2 PIR:S72555 huntingtin-associated protein HAP1 - human (fragment), N = 1, Score = 234, P = 8.6e-19 TREMBL :CEUT27A3_7 gene: "T27A3.1"; Caenorhabditis elegans cosmid T27A3., N = 1, Score = 226, P = 9.9e-16 PIR:S67495 huntingtin-associated protein HAP1-A - rat, N = 1, Score = 215, P = 1.6e-14 >PIR:S72555 huntingtin-associated protein HAP1 - human (fragment) Length = 320 HSPs: Score = 234 (35.1 bits), Expect = 8.6e-19, P = 8.6e-19 Identities = 66/189 (34%), Positives = 110/189 (58%) Query: 109 EESEPESVCSTPLKRNE—SSSSVQNYFH LDSLQKKLKDLEEENVVLRSEASQLKTE 163 EE+E + C+ P + S ++ + H L++LQ+KL+ LEEEN LR EASQL T Sbjct: 28 EEAEEDLQCAHPCDAPKLISQEALLHQHHCPQLEALQEKLRLLEEENHQLREEASQLDT- 86 Query: 164 TITYEEKEQQLVNDCVKELRDANVQIASISEELAKKTEDAARQQEEITHLLSQIVDLQKK 223 E++EQ L+ +CV++ +A+ Q+A +SE L + E+ RQQ+E+ L +Q++ LQ++ Sbjct: 87 LEDEEQMLILECVEQFSEASQQMAELSEVLVLRLENYERQQQEVARLQAQVLKLQQR 143 Query: 224 AKACAVENEELVQHLGAAKDAQRQLTAE—LRELEDKYAECME--MLHEAQEELKNL-RN 278 + E E+L + L + K+ Q QL E L ++ AE + + + + + RN Sbjct: 144 CRMYGAETEKLQKQLASEKEIQMQLQEEETLPGFQETLAEELRTSLRRMISDPVYFMERN 203 Query: 279 KTMP--NTTSRRY 289 MP +T+S RY Sbjct: 204 YEMPRGDTSSLRY 216 Peptide information for frame 3 ORF from 1416 bp to 1874 bp; peptide length: 153 Category: similarity to known protein Classification: unset 1 MSGVRSRGRR APPGSHDLET ALRRLSLRRE NYLSERRFFE EEQERKLQEL 51 AEKGELRSGS LTPTESIMSL GTHSRFSEFT GFSGMSFSSR SYLPEKLQIV 101 KPLEGDHAGP RPLSVLLGDS LWSLIHLRKA GHLCHAYΞFF FRDSHPRCWF 151 EFL BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfkd2_4c8, frame 3 TREMBL:AB011121_1 gene: "KIAA0549"; product: "KIAA0549 protein"; Homo sapiens mRNA for KIAA0549 protein, partial eds., N = 1, Score = 252, P = 5.5e-21 >TREMBL:AB011121_1 gene: "KIAA0549"; product: "KIAA0549 protein"; Homo sapiens mRNA for KIAA0549 protein, partial eds. Length = 469 HSPs: Score = 252 (37.8 bits), Expect = 5.5e-21, P = 5.5e-21 Identities = 57/98 (58%), Positives = 69/98 (70%) Query 8 GRRAPPGSHDLETALRRLSLRRENYLSERRFFEEEQERKLQELAEKGELRSGSLTPTESI 67 G+ P G DL TAL RLSLRR+NYLSE++FF EE +RK+Q LA++ E SG +TPTES+ Sbjct 27 GQPGPSGDSDLATALHRLSLRRQNYLSEKQFFAEEWQRKIQVLADQKEGVSGCVTPTESL 86 Query 68 MSLGTHSRFSEFTGFSGMSFSSRSYLPEKLQIVKPLEG 105 SL T SE T S S R ++PEKLQIVKPLEG Sbjct 87 ASLCTTQ—SEITDLSSAΞ-CLRGFMPEKLQIVKPLEG 121 Pedant information for DKFZphfkd2_4c8, frame 2 Report for DKFZphfkd2_4c8.2 [LENGTH] 442 [MW] 50020.14 [pi] 4.77 [HOMOL] TREMBL :AF040723_1 product: "neuroanl' Homo sapiens neuroanl mRNA, complete eds. 5e-29 [FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YDL058w] 5e-08 [FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YIL149c] 5e-08 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YDL058w] 5e-08 [FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YILl38c] 6e-08 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YGR130c] 2e-07 [ FUNCATj 09.10 nuclear biogenesis [S. cerevisiae, YDR356w] le-06 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YDR356w] le-06 [FUNCAT] 1 genome replication, transcription, recombination and repair [M. annaschii MJ1643] le-06 [FUNCAT] 08.22 cytoskeleton-dependent transport [S. cerevisiae, YHR023w MYOl - myosιn-1 soform] 3e-06 [FUNCAT] 03.25 cytokinesis [S. cerevisiae, YHR023w MYOl - myosιn-1 isoform] 3e-06 [FUNCAT] 11.04 dna repair (direct repair, base excision repair and nucleotide excision repair) [S. cerevisiae, YKR095w] 4e-06 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YKR095w] 4e-06 [FUNCAT] 03.13 meiosis [S. cerevisiae, YNL250w] 2e-05 [FUNCAT] 03.19 recombination and dna repair [S. cerevisiae, YNL250w] 2e-05 [FUNCAT] 08.99 other intracellular-transport activities [S. cerevisiae, YNL079c] 5e-05 FUNCAT] 03.01 cell growth [S. cerevisiae, YNL079c] 5e-05 FUNCAT] 03.07 pheromone response, mating-type determination, sex-specific proteins [S. cerevisiae, YNL079c] 5e-05 FUNCAT] 10.05.99 other pheromone response activities [S. cerevisiae, YHR158c] e-04 FUNCAT] 30.13 organization of chromosome structure [S. cerevisiae, YDR285w] le-04 FUNCAT] 30.09 organization of intracellular transport vesicles [S. cerevisiae, YNL272C] 3e-04 FUNCAT] 08.16 extracellular transport [S. cerevisiae, YNL272c] 3e-04 BLOCKS] BL01289B BLOCKS] BL00415M Synapsms proteins EC] 3.6.1.32 Myosin ATPase 2e-07 PIRKW] tandem repeat 2e-07 PIRKW] heterodimer le-06 PIRKW] endocytosis 9e-07 PIRKW] heart le-06 PIRKW] transmembrane protein 4e-07 PIRKW] zinc finger 9e-07 PIRKW] metal binding 9e-07 PIRKW] DNA binding 3e-06 PIRKW] muscle contraction 2e-07 PIRKW] acetylated ammo end 3e-06 PIRKW] actin binding 2e-07 PIRKW] mitosis le-06 PIRKW] microtubule binding le-06 PIRKW] ATP 2e-07 PIRKW] chromosomal protein le-06 PIRKW] receptor 3e-08 PIRKW] thick filament 2e-07 PIRKW] phosphoprotem 8e-06 PIRKW] glycoprotein 3e-08 PIRKW] skeletal muscle 3e-06 PIRKW] DNA condensation le-06 PIRKW] alternative splicing 2e-06 PIRKW] coiled coil 2e-07 PIRKW] P-loop 2e-07 PIRKW] heptad repeat 4e-07 PIRKW] methylated amino acid 2e-07 PIRKW] peripheral membrane protein 9e-07 PIRKW] cardiac muscle 6e-06 PIRKW] hydrolase 2e-07 PIRKW] muscle 2e-06 PIRKW] cytoskeleton 2e-06 PIRKW] Golgi apparatus 4e-07 PIRKW] calmodulin binding 9e-07 SUPFAM] myosin motor domain homology 2e-07 SUPFAM] tropomyosm TPM1 2e-06 SUPFAM] giantm 4e-07 SUPFAM] protein kinase C zinc-binding repeat homology 2e-06 SUPFAM] human early endosome antigen 1 9e-07 SUPFAM] unassigned kmesm-related proteins 4e-07 SUPFAM] M5 protein 8e-08 SUPFAM] cytoskeletal keratin 3e-06 SUPFAM] myosin heavy chain 2e-07 SUPFAM] conserved hypothetical P115 protein le-06 SUPFAM] centromere protein E le-06 SUPFAM] pleckstπn repeat homology 2e-06 SUPFAM] kinesin motor domain homology 4e-07 PROSITE] LEUCINE_ZIPPER 1 KW] All_Alpha KW] LOW_COMPLEXITY 6.79 % KW] COILED COIL 27.15 % SEQ MQKFIEADYYELDWYYEECSDVLCAERVGQMTKTYNDIDAVTRLLEEKERDLELAARIGQ SEG xxxxxxxxxxxxxxx ... PRD ccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS C SEQ SLLKKNKTLTERNELLEEQVEHIREEVSQLRHELSMKDELLQFYTSAAEESEPESVCSTP SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC SEQ LKRNESSSSVQNYFHLDSLQKKLKDLEEENVVLRSEASQLKTETITYEEKEQQLVNDCVK SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC SEQ ELRDANVQIASISEELAKKTEDAARQQEEITHLLSQIVDLQKKAKACAVENEELVQHLGA SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCC SEQ AKDAQRQLTAELRELEDKYAECMEMLHEAQEELKNLRNKTMPNTTSRRYHSLGLFPMDSL SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC SEQ AAEIEGTMRKELQLEEAESPDITHQKRVFETVRNINQVVKQRSLTPSPMNIPGSNQSSAM SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccccccchhhh COILS SEQ NSLLSSCVSTPRSSFYGSDIGNVVLDNKTNSIILETEAADLGNDERSKKPGTPGTPRLPR SEG xxxxxxxxxxx PRD hhhhhcccccccccccccccceeeeeccccceeecccccccccccccccccccccccccc COILS SEQ PGDGAEAAVPAPGELPLGEEVL SEG xxxx PRD cccccccccccccccccccccc COILS Prosite for DKFZphfkd2_4c8.2 PS00029 139->161 LEUCINE_ZIPPER PDOC00029 (No Pfam data available for DKFZphfkd2_4c8.2) Pedant information for DKFZphfkd2_4c8, frame 3 Report for DKFZphfkd2_4c8.3 [LENGTH] 153 [MW] 17642.03 [pi] 9.38 [HOMOL] TREMBL:AB011121_1 gene: "KIAA0549"; product: "KIAA0549 protein"; Homo sapiens mRNA for KIAA0549 protein, partial eds. 2e-12 [KW] Alpha_Beta [KW] LOW_COMPLEXITY 12.42 % SEQ MSGVRSRGRRAPPGSHDLETALRRLSLRRENYLSERRFFEEEQERKLQELAEKGELRSGS SEG xxxxxxxxxxxxxxxxxxx PRD cccccccccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccc SEQ LTPTESIMSLGTHSRFSEFTGFSGMSFSSRSYLPEKLQIVKPLEGDHAGPRPLSVLLGDS SEG PRD cccccceeeccccceeeccccccccccccccccchhhhhhhhcccccccccceeeeeccc SEQ LWSLIHLRKAGHLCHAYSFFFRDSHPRCWFEFL SEG PRD chhhhhhhhhcccccceeeeecccccccccccc (No Prosite data available for DKFZphfkd2_4c8.3) (No Pfam data available for DKFZphfkd2_4c8.3) DKFZphfkd2_4kl4 group: intracellular transport and trafficking DKFZphfkd2_4kl4.3 encodes a novel 254 amino acid putative GTP-bmdmg protein nearly identical to Rab6. Rab proteins are members of the Ras superfamily of GTPases. Rab proteins are localised to the cytoplasmic side of organelles and vesicles involved in the secretory (biosynthetic) and endocytotic pathways in eukaryotic cells. Rab proteins direct the targeting and fusion of transport vesicles to their acceptor membranes. rab6 is a ubiquitous ras-like GTPase involved in mtra-Golgi transport. The new protein can find application in modulating the transport of vesicles inside the Golgi apparatus . strong similarity to Rab6 complete cDNA, complete eds, EST hits Sequenced by GBF Locus: unknown Insert length: 3084 bp Poly A stretch at pos. 3061, polyadenylation signal at pos. 3043 1 GGGGCACTCA GCAGGTTGGG CTGCGGCGGC GGCGGCTGGG GAAGCCGAAG 51 CGCCGCGCGT GAGAGATCCC GGATACATCT GCGGTTTGGG CTCCGCCACC 101 CTCCGTCTCT CTCCCGCAGG TCTCTGAGCC GGGTGCGGAA GGAGGGAACG 151 GCCCTAGCCT TGGGAAGCCA AAGCACACCC CTGGCTCCCG CCGACACCGC 201 CCTCCTTCCC TTCCCAGCCG CGGGCCTCGC TCCGTGCTCG GCTACTCTGC 251 CGGGAGGCGG CGGCGGCTGC CAGTCTGTGG CGAGCCCTGC TGCCCTCCAG 301 CCGGGCTTCT CCAGCCGGGC TCCTCCACCG GCCCTTGCAG GGGCACAGAG 351 AGCTCGGCGC CCGCCCTTCC GCTCGCCTTT TTCGTCAGCC GGCTGGAGGA 401 GCATCGGTCC GGGAGGTCTC TGGGCTGAGG CGGCGACAGC TCCTCTAGTT 451 CCACCATGTC CGCGGGCGGA GACTTCGGGA ATCCGCTGAG GAAATTCAAG 501 CTGGTGTTCC TGGGGGAGCA AAGCGTTGCA AAGACATCTT TGATCACCAG 551 ATTCAGGTAT GACAGTTTTG ACAACACCTA TCAGGCAATA ATTGGCATTG 601 ACTTTTTATC AAAAACTATG TACTTGGAGG ATGGAACAAT CGGGCTTCGG 651 CTGTGGGATA CGGCGGGTCA GGAACGTCTC CGTAGCCTCA TTCCCAGGTA 701 CATCCGTGAT TCTGCTGCAG CTGTAGTAGT TTACGATATC ACAAATGTTA 751 ACTCATTCCA GCAAACTACA AAGTGGATTG ATGATGTCAG AACAGAAAGA 801 GGAAGTGATG TTATCATCAC GCTAGTAGGA AATAGAACAG ATCTTGCTGA 851 CAAGAGGCAA GTGTCAGTTG AGGAGGGAGA GAGGAAAGCC AAAGGGCTGA 901 ATGTTACGTT TATTGAAACT AGGGCAAAAA CTGGATACAA TGTAAAGCAG 951 CTCTTTCGAC GTGTAGCAGC AGCTTTGCCG GGAATGGAAA GCACACAGGA 1001 CGGAAGCAGA GAAGACATGA GTGACATAAA ACTGGAAAAG CCTCAGGAGC 1051 AAACAGTCAG CGAAGGGGGT TGTTCCTGCT ACTCTCCCAT GTCATCTTCA 1101 ACCCTTCCTC AGAAGCCCCC TTACTCTTTC ATTGACTGCA GTGTGAATAT 1151 TGGCTTGAAC CTTTTCCCTT CATTAATAAC GTTTTGCAAT TCATCATTGC 1201 TGCCTGTCTC GTGGAGGTGA TCTATTAGCT TCACAAGCAC AAAAAAAGTC 1251 AGCGTCTTCA TTATTTATAT TTTACAAAAA GCCAAATTAT TTCAGCATAT 1301 TCCGGTGATA ACTTTAAAAA TTAGATACAT TTTCTTAACA TTTTTTTCTT 1351 TTTTAATGTT ATGATAATGT ACTTCAAAAT GATGGAAATC TCAACAGTAT 1401 GAGTATGGCT TGGTTAACGA GCAGTATGTT CACAGCCTGC TTTATCTCTC 1451 CTTGCTCTTC TCACCTCTCC CTTACCCCGT TCCCTATTTC CGTGTTCTTA 1501 CCTAGCCTCC CCCCACTTCC TCAAAACAAA CAAGAGATGG CAAAGCAGCA 1551 GTCCGACCAA GCCCACTGGA ATTATCCTTT AATTTTACAG ATACCACTTG 1601 CTGTAGGCTG TGGACCAAGA TGTCCAGAAT TATTCTTGAG CACTGATGTA 1651 AATTACTTAG ATCTTCTTTG AGGTCAGAAT TCAGCGATCA CGGTAGGCAG 1701 TGCTTGAATG AGAAAAGCCT CCTGGTGCAT CTTCAAAATG AGTCCTAAAG 1751 AACATACTGA GTACTTATAA GTAGCAGAAC ATAAAATGTA TTTCTGACTA 1801 ACACAAATGG TCCTTTCACA TGTGCTTTAT TAGACTCTGG GAGAGAAAAG 1851 TAACCAAGTG CTTCAGAACA GGTTTTTAGT ATTTACTTCT TCATGGTAAG 1901 ATAATGAAGT TCTAATGAAC TATTTCTCCC AAGGTTTTAA AATTGTCAAG 1951 AGTTATTCTG TTTGTTTAAA AAGTAAGAAA CCTCTGTAAG CAATAGATTT 2001 TGCTTGGGTT TTCTTTCTTA AAAAAATAAT ACTATGCAGG CAAGACACCA 2051 TAAAAGTTTA ATTCCTTACA GAAGAACCAG TGGAAGAATT TAAATTTGGC 2101 ACTACGATCA AAACTACTGA ATTAGCAGAA ATAACGATAT CTAAAGCTTA 2151 CCAGCAAAAG AACCCTCAGC AGAATAGCAA AAACTTTGCT CAGGACATTT 2201 GAGGTCAAAT TGAAGACGGA AGACGGAAAC CGGAAACCGT TTTCTTGTAA 2251 GCCCCTAGAG GCAGATCAGG TAAGCATACA TAGTAGAGGG AAAGGAGAGA 2301 ATGGAAATAA AACTGAATAT TATGCAGATT TATGCCTTAT TTTTTAGCAT 2351 TTTTTAAGGT TGGGTCTTTC AGGCTGGTTT TGGTTTGTAT TAGATCTGTA 2401 TAGTTTAGTG ATTTAGTTTT ATATTTAAGC TACGATTAAT ATTTTTTCTT 2451 TGGCGATATT TCTTTGCTTT TTTTTTTTAA CAACTTTCCA TTTTTAGATG 2501 TTTCGTTGAA TCTATTTAGA GCTTCACCAT GGCAATATGT ATTTCCCTTA 2551 AAACACTGCA AACAAATATA CTAGGAGTGT GCCCTTTTAA TCTTTACTAG 2601 TTATTGTGAG ACTGCTGTGT AAGCTAATAA ACACATTTGT AAAAACATTG 2651 TTTGCAGGAA GAAAACTTCG AGTTACAGGT CAGGAAAAGC CTGCTGAATT 2701 TATGTTGTAA ACGTTACTTA ACACAGTATA AAGATGAAAA GACAACAAAA 2751 GTATCTTCAT ACTTCCTCAT CCCCTCATTG CAACAAAACC TTAAACTGGG 2801 AGAACCTTAG TCCCCTCTCT TTCCTCTTCC TCCTCCACTT CCCACTTATT 2851 GCCACTTTGT AATATTCAGA GAGCACTTGG ATTATGGATC TGAATAGAGA 2901 AATGCTTACA GATAATCATT AGCCCACATA CCAGTAACTT ATACTTAAAG 2951 ATGGGATGGA GTTATAAAGT GCTTTTATAA TCCAATATAA TTGCTAAAGG 3001 CAAGGGTTGA CTCTTTGTTT TATTTTGACA TGGCATGTCC TGAAATAAAT 3051 ATTGGTTCAC TATGAAAAAA AAAAAAAAAA AAAA BLAST Results No BLAST result Medline entries 98382468: Rab proteins. 97203146: GTP-bound forms of rab6 induce the redistribution of Golgi proteins into the endoplasmic reticulum. Peptide information for frame 3 ORF from 456 bp to 1217 bp; peptide length: 254 Category: strong similarity to known protein Classif cation: unset Prosite motifs: BACTERIAL OPΞIN RET (45-57) 1 MΞAGGDFGNP LRKFKLVFLG EQSVAKTSLI TRFRYDSFDN TYQAIIGIDF 51 LSKTMYLEDG TIGLRLWDTA GQERLRSLIP RYIRDSAAAV VVYDITNVNS 101 FQQTTKWIDD VRTERGSDVI ITLVGNRTDL ADKRQVSVEE GERKAKGLNV 151 TFIETRAKTG YNVKQLFRRV AAALPGMEST QDGSREDMSD IKLEKPQEQT 201 VSEGGCΞCYS PMSSSTLPQK PPYSFIDCSV NIGLNLFPSL ITFCNSSLLP 251 VSWR BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphf d2_4kl4, frame 3 PIR:G34323 GTP-binding protein Rab6 - human, N = 1, Score = 944, P = 6.5e-95 TREMBL:CET25G12_2 gene: "T25G12.4"; Caenorhabditis elegans cosmid T25G12., N = 1, Score = 756, P = 5.4e-75 TREMBL : NTNTRAF_1 gene: "Nt-rab6"; Nicotiana tabacum SRI Nt-rab6 mRNA, complete eds., N = 1, Score = 698, P = 7.6e-69 TREMBL :D8431 _1 product: "rab6"; Drosophila melanogaster mRNA for rab6, complete eds., N = 1, Score = 836, P = 1.9e-83 PIR:T01588 small GTP-binding protein F16B22.10 - Arabidopsis thaliana, N = 1, Score = 704, P = 1.8e-69 >PIR:G34323 GTP-bmdmg protein Rab6 - human Length = 208 HSPs: Score = 944 (141.6 bits), Expect = 6.5e-95, P = 6.5e-95 Identities = 186/208 (89%), Positives = 190/208 (91%) Query: 1 MSAGGDFGNPLRKFKLVFLGEQSVAKTSLITRFRYDΞFDNTYQAIIGIDFLSKTMYLEDG 60 MS GGDFGNPLRKFKLVFLGEQSV KTSLITRF YDSFDNTYQA IGIDFLSKTMYLED Sbjct: 1 MSTGGDFGNPLRKFKLVFLGEQSVGKTSLITRFMYDSFDNTYQATIGIDFLSKTMYLEDR 60 Query: 61 TIGLRLWDTAGQERLRSLIPRYIRDSAAAVVVYDITNVNSFQQTTKWIDDVRTERGSDVI 120 T+ L+LWDTAGQER RSLIP YIRDS AVVVYDITNVNSFQQTTKWIDDVRTERGSDVI Sbjct: 61 TVRLQLWDTAGQERFRSLIPSYIRDSTVAVVVYDITNVNSFQQTTKWIDDVRTERGSDVI 120 Query: 121 ITLVGNRTDLADKRQVSVEEGERKAKGLNVTFIETRAKTGYNVKQLFRRVAAALPGMEST 180 I LVGN+TDLADKRQVS+EEGERKAK LNV FIET AK GYNVKQLFRRVAAALPGMEST Sbjct: 121 IMLVGNKTDLADKRQVSIEEGERKAKELNVMFIETSAKAGYNVKQLFRRVAAALPGMEST 180 Query: 181 QDGΞREDMSDIKLEKPQEQTVSEGGCSC 208 QD SREDM DIKLEKPQEQ VSEGGCSC Sbjct: 181 QDRSREDMIDIKLEKPQEQPVSEGGCSC 208 Pedant information for DKFZphfkd2_4kl4, frame 3 Report for DKFZphfkd2_4kl4.3 [LENGTH] 254 [MW] 28385.29 [pi] 7.58 [HOMOL] PIR:G34323 GTP-bmding protein Rab6 - human le-102 [FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YLR262c] 7e-60 [FUNCAT] 30.08 organization of golgi [S. cerevisiae, YLR262c] 7e-60 [FUNCAT] 30.09 organization of intracellular transport vesicles [S. cerevisiae, YOR089C] 2e ■ 33 [FUNCAT] 08.19 cellular import [S. cerevisiae, YOR089c] 2e-33 [FUNCAT] 08.13 vacuolar transport [S. cerevisiae, YOR089c] 2e-33 [FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YOR089c] 2e-33 [FUNCAT] 09.09 biogenesis of intracellular transport vesicles [S. cerevisiae, YGL210W] 3e •28 [FUNCAT] 30.02 organization of plasma membrane [S. cerevisiae, YFL005w] 8e-27 [FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YFL005w] 8e-27 [FUNCAT] 01.05.04 regulation of carbohydrate utilization [S. cerevisiae, YORlOlw] 2e-21 [FUNCAT] 11.10 cell death [S. cerevisiae, YORlOlw] 2e-21 [FUNCAT] 01.03.13 regulation of nucleotide metabolism [S. cerevisiae, YORlOlw] 2e-21 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YORlOlw] 2e-21 [FUNCAT] 03.99 other cell growth, cell division and dna synthesis activities [S. cerevisiae, YORlOlw] 2e-21 [FUNCAT] 10.04.07 g-proteins [S. cerevisiae, YORlOlw] 2e-21 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YNL098C] 6e-19 [FUNCAT] 11.01 stress response [S. cerevisiae, YNL098c] 6e-19 [FUNCAT] 03.10 sporulation and germination [S. cerevisiae, YNL098C] 6e-19 [FUNCAT] 04.07 rna transport [S. cerevisiae, YOR185c] 6e-16 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YOR185c] 6e-16 [FUNCAT] 08.01 nuclear transport [S. cerevisiae, YOR185C] 6e-16 [FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YPR165w] 4e-13 [FUNCAT] 10.02.07 g-protems [S. cerevisiae, YPR165w] 4e-13 [FUNCAT] 10.99 other signal-transduction activities [S. cerevisiae, YCR027c] 2e-09 [FUNCAT] 10.05.07 g-protems [S. cerevisiae, YLR229C] 8e-08 [FUNCAT] 03.07 pheromone response, mating-type determination, sex-specific proteins [Ξ. cerevisiae, YLR229C] 8e-08 [FUNCAT] 03.01 cell growth [S. cerevisiae, YNL180c] le-05 [FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YOR094w] 5e-05 [BLOCKS] BL01115A GTP-bmding nuclear protein ran proteins [SCOP] dlas3_2 3.29.1.4.12 Transduem (alpha subunit), insertion domai le-32 [SCOP] dlmhl 3.29.1.4.2 Racl [Human (Homo sapiens) 2e-51 [SCOP] d5p21 3.29.1. 1 cH-p21 Ras protein [human (Homo sapiens) 7e-53 [SCOP] dlhura_ 3.29.1. 8 ADP-ribosylation factor 1 (ARFl) [human (Hom le-46 [SCOP] dla2kc_ 3.29.1. 5 Ran Nuclear transport factor-2 (NTF2) [Do 6e-60 [PIRKW] nucleus 2e-14 [PIRKW] cell cycle control 5e-15 [PIRKW] membrane trafficking 3e-71 [PIRKW] endoplasmic reticulum le-29 [PIRKW] phosphoprotein le-29 [PIRKW] prenylated cysteine 2e-36 [PIRKW]. signal transduction 5e-15 [PIRKW] transforming protein 5e-30 [PIRKW] puπne nucleotide binding le-26 [PIRKW] alternative splicing le-18 [PIRKW] P-loop 3e-71 [PIRKW] lipoprotein 2e-36 [PIRKW] proto-oncogene le-20 [PIRKW] methylated carboxyl end le-20 [PIRKW] membrane protein le-29 [PIRKW] GTP binding 3e-71 [PIRKW] thiolester bond le-29 [PIRKW] Golgi apparatus le-29 [SUPFAM] ras transforming protein le-76 [PROSITE] BACTERIAL_OPSIN_RET 1 [PFAM] Ras family (contains ATP/GTP binding P-loop) [KW] Alpha_Beta [KW] 3D SEQ MSAGGDFGNPLRKFKLVFLGEQSVAKTSLITRFRYDSFDNTYQAIIGIDFLSKTMYLEDG lkao- CCEEEEEEECTTTTCHHHHHHHHHHCCCCCCCTTTTC-EEEEEEEEETTE SEQ TIGLRLWDTAGQERLRSLIPRYIRDSAAAVVVYDITNVNSFQQTTKWIDDVRTERGSDVI lkao- EEEEEEEECCTTTTCHHHHHHHHHHCCEEEEEEETTTHHHHHHHHHHHHHHHHHTTTCCC SEQ ITLVGNRTDLADKRQVSVEEGERKAKGLNVTFIETRAKTGYNVKQLFRRVAAALPGMEST lkao- EEEEEETTTTGGGCCCCHHHHHHHHHHHCCCEEECTTTTHHHHHHHHHHH SEQ QDGSREDMSDIKLEKPQEQTVSEGGCΞCYSPMSSSTLPQKPPYSFIDCSVNIGLNLFPSL lkao- SEQ ITFCNSSLLPVSWR lkao- Prosite for DKFZphfkd2_4kl4.3 PS00327 45->57 BACTERIAL OPSIN RET PDOC00291 Pfam for DKFZphfkd2_4kl4.3 HMM_NAME Ras family (contains ATP/GTP binding P-loop) HMM *KLVLIGDSGVGKSCLLIRFTQNeFnEeYIPTIGvDFYtKTIEIDGKtIK KLV++G+ +V K++L RF +++F++ Y + IG+DF++KT+++++ TI Query 15 KLVFLGEQSVAKTSLITRFRYDSFDNTYQAIIGIDFLSKTMYLEDGTIG 63 HMM LQIWDTAGQERYRsMRPMYYRGAMGFMLVYDITNRqSFENIrNWweEIrR L +WDTAGQER RS+ P Y+R++ ++++VYDITN SF+ ++W++++R+ Query 64 LRLWDTAGQERLRSLIPRYIRDSAAAVVVYDITNVNSFQQTTKWIDDVRT 113 HMM HCDrDENVPIMLVGNKCDLEDQRQVStEEGQeFAREWGAIPFMETSAKTN + ++V+I LVGN +DL+D+RQVS EEG+ A+ ++ + F+ET AKT+ Query 114 ERG—SDVIITLVGNRTDLADKRQVSVEEGERKAKGLN-VTFIETRAKTG 160 HMM iNVEEAFMEIvRellqrMqe.q.NqteNinidQpsrnrk....rCCCIM* +NV++ F +++ +++ +++ + +++++++I+ ++++ + +C+ + Query 161 YNVKQLFRRVAAALPGMESTQDGSREDMSDIKLEKPQEQTVSEGGCS-C 208 DKFZphfkd2_4mll group: transmembrane protein DKFZphfbr2-4mll encodes a novel 159 ammo acid protein with weak similarity to the putative membrane protein YMR034c of S. cerevisiae. The novel protein contains 4 transmembrane regions . No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of kidney-specific genes and as a new marker of neuronal cells. weak similarity to YMR034c complete cDNA, complete eds, no EST hits Sequenced by GBF Locus : unknown Insert length: 1749 bp Poly A stretch at pos. 1727, polyadenylation signal at pos. 1713 1 GGGGTCCTCA AAGCCGCCGG AGCAACCCCC AGGTCTTTAC TTTACAATCG 51 GCAATTTGAC TTGCTCTGCT GCATGTCTGG AGGGACCAAG GAAAGTGTGG 101 AGACGCTCCA AGGATTAGGT GATCGGAGCT TGAAAAGAAA AAAAGCCAAA 151 CAAATAAACA AAACCCACCC ACCCTAACGA ATATGAGGCT GCTGGAGAGA 201 ATGAGGAAAG ACTGGTTCAT GGTCGGAATA GTGCTGGCGA TCGCTGGAGC 251 TAAACTGGAG CCGTCCATAG GGGTGAATGG GGGACCACTG AAGCCAGAAA 301 TAACTGTATC CTACATTGCT GTTGCAACAA TATTCTTTAA CAGTGGACTA 351 TCATTGAAAA CAGAGGAGCT GACCAGTGCT TTGGTGCATC TAAAACTGCA 401 TCTTTTTATT CAGATCTTTA CTCTTGCATT CTTCCCAGCA ACAATATGGC 451 TTTTTCTTCA GCTTTTATCA ATCACACCCA TCAACGAATG GCTTTTAAAA 501 GGTTTGCAGA CAGTAGGTTG CATGCCTCCG CCTGTGTCTT CTGCAGTGAT 551 TTTAACCAAG GCAGTTGGTG GAAATGAGGC AGCTGCAATA TTTAATTCAG 601 CCTTTGGAAG TTTTTTGGTA AGTAAACATA GTTTAACTTG TCTATTACAA 651 CTTTTGCTGT GATATTGTGT ATATGAAAGA TTTAGTGAAA GCTGGATTTG 701 TTTTACTCTT TGGTTAAGTA TAAAAATTGT TGAATCTTTT CATGTGCCAG 751 TATCCATACC CTGAAGAAAA GTAGTTAATG AATAAAGCAA ATGTTCTCTT 801 ACAATATATT TTGGAGGTTT GGATTTTAAA ATTCCATTTA ATGAATTCAA 851 GGAATCAATT AAAACACTAT GTGTCTCCTT ATAGAGGTTA TGTCAATATA 901 TTGATCATTT AATGAGGTCT TTTAGATTAT TATTATTTTG TATCATGGGA 951 CTGAGGATTT TGAAAAGGAA ACATGACCCA GCTGGTCAGA AAGGGAATGC 1001 TAATTTACTT GTTGACATGC CATTTATTTT GTACATTTCA CTGTCAAAGA 1051 AGCTACTGGC TTGGATGCTT CTGAGAAATC TATGTGAGAA AAAATTTGAA 1101 AGGAAGATAT GACTAATGAG TAATTTGCAA GTAAATGTTG TATCTATATA 1151 TATATATATA TAAAGATTCA AAAGTAGTTC AGCTTTCATA AGTAGAACCA 1201 ATATAAGGAC GTTGTTTTAG CATTTTTAAT CATTATTTTT AAATAAATGA 1251 TGTAACAGAG GCTTGATTTG TGTTATGAAA GATTGAGAAA CTAAATTTTC 1301 TGTTGATTTA ATTTTTTTGT GCCTTAAAAC TTTGTTAAAT TCCTGAAGTT 1351 AATTATCATA TTGTACTTTT TGGGGCATAA CTCATTAGCA GATATGTAGT 1401 GCAGTGATTT ACAAATAATT GAGAGTAAAA TCAGTGATGT ATAAACTAGT 1451 TCATGAGTCT AGGTAAAATA TCAATTACCT CTGTTTAAAA TGCTCTGTTA 1501 ATTATTATTG TATGTATTTA AATGTAGTTA AAGCTTTTAA ACATGTTGTT 1551 ACATAGTGTT AATTCTACAC AGTGCTACAC AGCTTTTAGT GTCACATAGC 1601 CTTACAGAGT TTATAATGAT GTAGCATCTG CAAAATATAT GCATAGCTTA 1651 TATCCTATTT TTATAGAGCC AGTAATGGTT TTTGTGATGC TGTATTACTT 1701 CTGGGTTTTA GACAATAAAG TCTGTTTAAC AAAAAAAAAA AAAAAAAAA BLAST Results o BLAST result Medline entries o Medline entry Peptide information for frame 3 ORF from 183 bp to 659 bp; peptide length: 159 Category: similarity to unknown protein 1 MRLLERMRKD WFMVGIVLAI AGAKLEPSIG VNGGPLKPEI TVSYIAVATI 51 FFNSGLSLKT EELTSALVHL KLHLFIQIFT LAFFPATIWL FLQLLSITPI 101 NEWLLKGLQT VGCMPPPVSS AVILTKAVGG NEAAAIFNSA FGSFLVSKHS 151 LTCLLQLLL BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphfkd2_4mll, frame 3 PIR:S53951 probable membrane protein YMR034c - yeast (Saccharomyces cerevisiae), N = 1, Score = 171, P = 3.2e-12 PIR:A65015 yfeH protein - Escherichia coli (strain K-12), N = 1, Score = 131, P = 4.2e-08 >PIR:S53951 probable membrane protein YMR034c - yeast (Saccharomyces cerevisiae) Length = 434 HSPs: Score = 171 (25.7 bits), Expect = 3.2e-12, P = 3.2e-12 Identities = 38/144 (26%), Positives = 72/144 (50%) Query: 5 ERMRKDWFMVGIVLAIAGAKLEPSIGVNGGPLKPEITVSYIAVATIFFNSGLSLKTEELT 64 E ++ WF + + + I A+ P+ +GG +K + ++ Y VA IF SGL +K+ L Sbjct: 18 EFLKSQWFFICLAILIVIARFAPNFARDGGLIKGQYSIGYGCVAWIFLQSGLGMKSRSLM 77 Query: 65 SALVHLKLHLFIQIFTLAFFPATIWLF LQLLSITPINEWLLKGLQTVGCMPPPVSSA 121 + +++ + H I + + + ++ F ++ + I++W+L GL P V+S Sbjct: 78 ANMLNWRAHATILVLSFLITSSIVYGFCCAVKAANDPKIDDWVLIGLILTATCPTTVASN 137 Query: 122 VILTKAVGGNEAAAIFNSAFGSFL 145 VI+T GGN + G+ L Sbjct: 138 VIMTTNAGGNSLLCVCEVFIGNLL 161 Pedant information for DKFZphfkd2_4mll, frame 3 Report for DKFZphfkd2_4mll .3 [LENGTH] 159 [MW] 17282.92 [pi] 9.06 [HOMOL] PIR:S53951 probable membrane protein YMR034C - yeast (Saccharomyces cerevisiae) 5e-12 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YMR034c] 2e-13 [PROSITE] MYRISTYL 2 [PROSITE] PKC_PHOSPHO_SITE 1 [KW] TRANSMEMBRANE 4 SEQ MRLLERMRKDWFMVGIVLAIAGAKLEPSIGVNGGPLKPEITVSYIAVATI FFNSGLSLKT PRD ccchhhhhhhhhhhhhhhhhhhhhcccccccccccccceeeeeeeccccccccccchhhh MEM MMMMMMMMMMMMMMMMMMMMMMMMMM MMMMMMMMMMMMMMMMMMMMMM .. SEQ EELTSALVHLKLHLFIQIFTLAFFPATIWLFLQLLSITPINEWLLKGLQTVGCMPPPVSΞ PRD hhhhhhhhhhhhhhhhhhhhhhccchhhhhhhhhhccccchhhhhhhheeeecccccccc MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM SEQ AVILTKAVGGNEAAAIFNSAFGSFLVSKHSLTCLLQLLL PRD ceeeeeccccchhhhhhhcccccceeecceeeeeeeccc MEM MMMMMMMMMMMMMMMMMMMMMMMMMMM Prosite for DKFZphf d2_4mll .3 PS00005 57->60 PKC_PHOSPHO_SITE PDOC00005 PS00008 15->21 MYRISTYL PDOC00008 PS00008 129->135 MYRISTYL PDOC00008 (No Pfam data available for DKFZphfkd2_4mll .3) PAGE INTENTIONALLY LEFT BLANK DKFZphutel_17k7 group: uterus derived DKFZphutel_17k7 encodes a novel 520 amino acid protein with weak similarity to S. Cerevisiae Fipl. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of uterus-specific genes . similarity to S. cerevisiae Fipl complete cDNA, complete eds, EST hits Sequenced by BMFZ Locus: unknown Insert length: 1914 bp Poly A stretch at pos. 1897, polyadenylation signal at pos. 1867 1 CGGACGCGTG GGCGGACGCG TGGGGCCTTC CTGGGATTGG AGTCTCGAGC 51 TTTCTTCGTT CGTTCGCCGG CGGGTTCGCG CCCTTCTCGC GCCTCGGGGC 101 TGCGAGGCTG GGGAAGGGGT TGGAGGGGGC TGTTGATCGC CGCGTTTAAG 151 TTGCGCTCGG GGCGGCCATG TCGGCCGGCG AGGTCGAGCG CCTAGTGTCG 201 GAGCTGAGCG GCGGGACCGG AGGGGATGAG GAGGAAGAGT GGCTCTATGG 251 CGATGAAAAT GAAGTTGAAA GGCCAGAAGA AGAAAATGCC AGTGCTAATC 301 CTCCATCTGG AATTGAAGAT GAAACTGCTG AAAATGGTGT ACCAAAACCG 351 AAAGTGACTG AGACCGAAGA TGATAGTGAT AGTGACAGCG ATGATGATGA 401 AGATGATGTT CATGTCACTA TAGGAGACAT TAAAACGGGA GCACCACAGT 451 ATGGGAGTTA TGGTACAGCA CCTGTAAATC TTAACATCAA GACAGGGGGA 501 AGAGTTTATG GAACTACAGG GACAAAAGTC AAAGGAGTAG ACCTTGATGC 551 ACCTGGAAGC ATTAATGGAG TTCCACTCTT AGAGGTAGAT TTGGATTCTT 601 TTGAAGATAA ACCATGGCGT AAACCTGGTG CTGATCTTTC TGATTATTTT 651 AATTATGGGT TTAATGAAGA TACCTGGAAA GCTTACTGTG AAAAACAAAA 701 GAGGATACGA ATGGGACTTG AAGTTATACC AGTAACCTCT ACTACAAATA 751 AAATTACGGT ACAGCAGGGA AGAACTGGAA ACTCAGAGAA AGAAACTGCC 801 CTTCCATCTA CAAAAGCTGA GTTTACTTCT CCTCCTTCTT TGTTCAAGAC 851 TGGGCTTCCA CCGAGCAGGA GATTACCTGG GGCAATTGAT GTTATCGGTC 901 AGACTATAAC TATCAGCCGA GTAGAAGGCA GGCGACGGGC AAATGAGAAC 951 AGCAACATAC AGGTCCTTTC TGAAAGATCT GCTACTGAAG TAGACAACAA 1001 TTTTAGCAAA CCACCTCCGT TTTTCCCTCC AGGAGCTCCT CCCACTCACC 1051 TTCCACCTCC TCCATTTCTT CCACCTCCTC CGACTGTCAG CACTGCTCCA 1101 CCTCTGATTC CACCACCGGG TTTTCCTCCT CCACCAGGCG CTCCACCTCC 1151 ATCTCTTATA CCAACAATAG AAAGTGGACA TTCCTCTGGT TATGATAGTC 1201 GTTCTGCACG TGCATTTCCA TATGGCAATG TTGCCTTTCC CCATCTTCCT 1251 GGTTCTGCTC CTTCGTGGCC TAGTCTTGTG GACACCAGCA AGCAGTGGGA 1301 CTATTATGCC AGAAGAGAGA AAGACCGAGA TAGAGAGAGA GACAGAGACA 1351 GAGAGCGAGA CCGTGATCGG GACAGAGAAA GAGAACGCAC CAGAGAGAGA 1401 GAGAGGGAGC GTGATCACAG TCCTACACCA AGTGTTTTCA ACAGCGATGA 1451 AGAACGATAC AGATACAGGG AATATGCAGA AAGAGGTTAT GAGCGTCACA 1501 GAGCAAGTCG AGAAAAAGAA GAACGACATA GAGAAAGACG ACACAGGGAG 1551 AAAGAGGAAA CCAGACATAA GTCTTCTCGA AGTAATAGTA GACGTCGCCA 1601 TGAAAGTGAA GAAGGAGATA GTCACAGGAG ACACAAACAC AAAAAATCTA 1651 AAAGAAGCAA AGAAGGAAAA GAAGCGGGCA GTGAGCCTGC CCCTGAACAG 1701 GAGAGCACCG AAGCTACACC TGCAGAATAG GCATGGTTTT GGCCTTTTGT 1751 GTATATTAGT ACCAGAAGTA GATACTATAA ATCTTGTTAT TTTTCTGGAT 1801 AATGTTTAAG AAATTTACCT TAAATCTTGT TCTGTTTGTT AGTATGAAAA 1851 GTTAACTTTT TTTCCAAAAT AAAAGAGTGA ATTTTTCATG TTAAGTTAAA 1901 AAAAAAAAAA AAAA BLAST Results o BLAST result Medline entries o Medlme entry Peptide information for frame 3 ORF from 168 bp to 1727 bp; peptide length: 520 Category: similarity to known protein 1 MSAGEVERLV SELSGGTGGD EEEEWLYGDE NEVERPEEEN ASANPPSGIE 51 DETAENGVPK PKVTETEDDS DSDSDDDEDD VHVTIGDIKT GAPQYGSYGT 101 APVNLNIKTG GRVYGTTGTK VKGVDLDAPG SINGVPLLEV DLDSFEDKPW 151 RKPGADLSDY FNYGFNEDTW KAYCEKQKRI RMGLEVIPVT STTNKITVQQ 201 GRTGNSEKET ALPSTKAEFT SPPSLFKTGL PPSRRLPGAI DVIGQTITIS 251 RVEGRRRANE NSNIQVLSER SATEVDNNFS KPPPFFPPGA PPTHLPPPPF 301 LPPPPTVSTA PPLIPPPGFP PPPGAPPPSL IPTIESGHSS GYDSRSARAF 351 PYGNVAFPHL PGSAPSWPSL VDTSKQWDYY ARREKDRDRE RDRDRERDRD 401 RDRERERTRE RERERDHSPT PSVFNSDEER YRYREYAERG YERHRASREK 451 EERHRERRHR EKEETRHKSS RSNSRRRHES EEGDSHRRHK HKKSKRSKEG 501 KEAGSEPAPE QESTEATPAE BLASTP hits Entry AF016427_4 from database TREMBL: gene: "F32D1.9"; Caenorhabditis elegans cosmid F32D1. Score = 392, P = 1.8e-36, identities = 156/519, positives = 212/519 Entry S62454 from database PIR: hypothetical protein SPAC22G7.10 - fission yeast (Schizosaccharomyces pombe) Score = 246, P = 2.0e-22, identities = 62/163, positives = 91/163 Entry A56545 from database PIR: FIP1 protein - yeast (Saccharomyces cerevisiae) Score = 186, P = 2.9e-16, identities = 56/206, positives = 92/206 Alert BLASTP hits for DKFZphutel_17k7, frame 3 TREMBLNEW :AF109907_1 product: "S164"; Homo sapiens S164 gene, partial eds; PSl and hypothetical protein genes, complete eds; and S171 gene, partial eds., N = 2, Score = 236, P = 1.5e-16 >TREMBLNEW:AF109907_1 product: "S164"; Homo sapiens S164 gene, partial eds; PSl and hypothetical protein genes, complete eds; and S171 gene, partial eds . Length = 735 HSPs: Score = 236 (35.4 bits), Expect = 1.5e-16, Sum P(2) = 1.5e-16 Identities = 51/120 (42%), Positives = 76/120 (63%) Query: 383 REKDRDRERDRDRERDRDRDRERERTRERERERDHSPTPSVFNSDEERYRYREYA ER 439 REK+++RER+R+R+RDRDR +ER+R R+RER+RD S + +++R R RE + ER Sbjct: 227 REKEKERERERERDRDRDRTKERDRDRDRERDRDRDRERSS-DRNKDRSRSREKSRDRER 285 Query: 440 GYERHRASREKEERHRER-RHREKEETRHKSSRSNSRRRHESEEGDSHRRHKHKKSKRSK 498 ER R + ER RER R RE+E R + + +R E +E D++ R K ++ R K Sbjct: 286 EREREREREREREREREREREREREREREREREKDKKRDREEDEEDAYERRKLERKLREK 345 Query: 499 E 499 E Sbjct: 346 E 346 Score = 214 (32.1 bits), Expect = 4.4e-14, Sum P(2) = 4.4e-14 Identities = 50/133 (37%), Positives = 75/133 (56%) Query: 383 REKDRDR-ERDRDRERDRDRDRERERTRERERERDHSPTPSVFNS-DEERYRYREYAERG 440 RE++R+R ER+R+RER+R+R++E+ER RERER+RD T D ER R R+ ER Sbjct: 208 RERERERREREREREREREREKEKERERERERDRDRDRTKERDRDRDRERDRDRD-RERS 266 Query: 441 YERHRASREKEERHRERRHREKEETRHKSSRSNSRRRHESEEGDSHRRHKHKKSKRSKEG 500 +R++ E+ R+R RE+E R + R R R E + R + ++ K K Sbjct: 267 SDRNKDRSRSREKSRDRE-RERERERERE-REREREREREREREREREREREREKDKKRD 324 Query: 501 KEAGSEPAPEQESTE 515 +E E A E+ E Sbjct: 325 REEDEEDAYERRKLE 339 Score = 214 (32.1 bits), Expect = 4.4e-14, Sum P(2) = 4.4e-14 Identities = 55/141 (39%), Positives = 80/141 (56%) Query: 383 REKDRDR-ERDRDRERDRDRDRERERTRERERERDHSPTPSVFNS-DEERYRYREYAERG 440 RE++R+R ER+R+RER+R+R++E+ER RERER+RD T D ER R R+ ER Sbjct: 208 RERERERREREREREREREREKEKERERERERDRDRDRTKERDRDRDRERDRDRD-RERS 266 Query: 441 YERHR-ASREKEE-RHRER-RHREKEETRHKSSRSNSRRRHESEEGDSHRRHKHKKSKRS 497 +R++ SR +E+ R RER R RE+E R + R E E R K KK R Sbjct: 267 SDRNKDRSRSREKSRDREREREREREREREREREREREREREREREREREREKDKKRDRE 326 Query: 498 KEGKEAGSEPAPEQESTEATPA 519 ++ ++A E++ E A Sbjct: 327 EDEEDAYERRKLERKLREKEAA 348 Score = 210 (31.5 bits), Expect = 1.2e-13, Sum P(2) = 1.2e-13 Identities = 59/142 (41%), Positives = 78/142 (54%) Query: 383 REKDRDRERDRDRERDRDRDRERERTRERERERDHSPTPSVFNS DEERYRYREYAER 439 RE++RDR+RDR +ERDRDRDRER+R R+RER D + S D ER R RE ER Sbjct: 235 RERERDRDRDRTKERDRDRDRERDRDRDRERSSDRNKDRSRSREKSRDRERERERE-RER 293 Query: 440 GYERHRA-SREKE-ERHRER-RHREKEETRHKSS RSNSRRRHESEEGDSHRRH 489 ER R RE+E ER RER R REK++ R + R R+ +E R Sbjct: 294 EREREREREREREREREREREREREKDKKRDREEDEEDAYERRKLERKLREKEAAYQERL 353 Query: 490 KHKKSKRSKEGKEAGSEPAPEQE 512 K+ + + K+ +E E E+E Sbjct: 354 KNWEIRERKKTREYEKEAEREEE 376 Score = 205 (30.8 bits), Expect = 4.4e-13, Sum P(2) = 4.4e-13 Identities = 59/149 (39%), Positives = 83/149 (55%) Query: 372 DTSKQWDYYARREKDRDR—ERDRDRERDRDRDRERERTRERERERDHSPTPSVFNSDEE 429 + K+ + R++DRDR ERDRDR+R+RDRDR+RER+ +R ++R S S D E Sbjct: 228 EKEKERERERERDRDRDRTKERDRDRDRERDRDRDRERSSDRNKDRSRSREKS RDRE 284 Query: 430 RYRYREYAERGYERHRA-SREKE-ERHRER-RHREKEETRHKSS RSNSRRRHE 479 R R RE ER ER R RE+E ER RER R REK++ R + R R+ Sbjct: 285 RERERE-REREREREREREREREREREREREREREKDKKRDREEDEEDAYERRKLERKLR 343 Query: 480 SEEGDSHRRHKHKKSKRSKEGKEAGSEPAPEQE 512 +E R K+ + + K+ +E E E+E Sbjct: 344 EKEAAYQERLKNWEIRERKKTREYEKEAEREEE 376 Score = 202 (30.3 bits), Expect = 9.6e-13, Sum P(2) = 9.6e-13 Identities = 49/117 (41%), Positives = 70/117 (59%) Query: 383 REKDRDRERDRDRERDRDRDRERERTRERERERDHSPTPSVFNSDEERYRYREYAERGYE 442 REK RDRER+R+RER+R+R+RERER RERERER+ D++R R E E YE Sbjct: 277 REKSRDRERERERERERERERERERERERERERERERERER-EKDKKRDR-EEDEEDAYE 334 Query: 443 RHRASREKEERHRERRHREKEETRHKSSRSNSRR-RHESEEGDSHRRHKHKKSKRSKE 499 R + E++ R +E ++E+ + R +R E+E + RR K++KR KE Sbjct: 335 RRKL—ERKLREKEAAYQERLKNWEIRERKKTREYEKEAEREEERRREMAKEAKRLKE 390 Score = 183 (27.5 bits), Expect = 1.2e-10, Sum P(2) = 1.2e-10 Identities = 52/141 (36%), Positives = 79/141 (56%) Query: 372 DTSKQWDYY-ARREKDRDR-ERDRDRERDRDRDRERERTRERERERDHSPTPSVFNSDEE 429 DT K+ + ++EK+R E++R RER+R+R+RERER RERERER+ ++E Sbjct: 178 DTHKKLEEEKGKKEKERQEIEKER-RERERERERERER-RERERERERER EREKE 230 Query: 430 RYRYREYAERGYERHRASREKEERHRER RHREKEETRHKSSRSNSRRRHESEEGDSH 486 + R RE ER +R R +R RER R RE+ R+K RS SR + E + Sbjct: 231 KERERE-RERDRDRDRTKERDRDRDRERDRDRDRERSSDRNKD-RSRSREKSRDRERERE 288 Query: 487 RRHKHKKSKRSKEGKEAGSEPAPEQE 512 R + ++ + + +E E E+E Sbjct: 289 RERERERERERERERERERERERERE 314 Score = 171 (25.7 bits), Expect = 2.5e-09, Sum P(2) = 2.5e-09 Identities = 49/150 (32%), Positives = 78/150 (52%) Query: 383 REKDRDRERDRDRERDRDRDRERERTRERERERDHSPTPSVFNSDEERYRYREYAERGYE 442 RE++R+RER+R+RER+R+R+RERER RERERER+ +E+ Y R+ + E Sbjct: 285 REREREREREREREREREREREREREREREREREKDKKRDREEDEEDAYERRKLERKLRE 344 Query: 443 RHRASREK EERHRERRHR EKEETRHKSSRSNSRRRHES-EEGDSHRRH-KH 491 + A +E+ ER + R + E+EE R + ++R E E+ D R K+ Sbjct: 345 KEAAYQERLKNWEIRERKKTREYEKEAEREEERRREMAKEAKRLKEFLEDYDDDRDDPKY 404 Query: 492 KKSKRSKEGKEAGSEPAPEQESTE 515 +K R +E + E ++E E Sbjct: 405 YRGSALQKRLRDREKEMEADERDRKREKEE 434 Score = 162 (24.3 bits), Expect = 2.4e-08, Sum P(2) = 2.4e-08 Identities = 45/141 (31%), Positives = 74/141 (52%) Query: 372 DTSKQWDYYARREKDRDRERDRDRERDRDRDRERERTRERERERDHSPTPSVFNSDEERY 431 + SK D + + E+++ ++ +E +++R RERER RERERER + ER Sbjct: 172 EISKFRDTHKKLEEEKGKKEKERQEIEKER-RERERERERERERRERERER--ERERERE 228 Query: 432 RYREYAERGYERHRASREKEERHRER-RHREKEETRHKSSRSNSRRRHESEEGDSHRRHK 490 + +E ER ER R +ER R+R R R+++ R +SS N R E+ R + Sbjct: 229 KEKE-RERERERDRDRDRTKERDRDRDRERDRDRDRERSSDRNKDRSRSREKSRDRERER 287 Query: 491 HKKSKRSKEGKEAGSEPAPEQE 512 ++ +R +E +E E E+E Sbjct: 288 ERERERERE-RERERERERERE 308 Score = 137 (20.6 bits), Expect = 1.2e-05, Sum P(2) = 1.2e-05 Identities = 48/152 (31%), Positives = 68/152 (44%) Query: 364 APSWPSLVDTSKQWDYYARREKDRDR-ERDRDRERDRDRDRERERTRERERERDHSPTPS 422 AP P + T + + E RD R+ + RD + E E+ + +E+ER Sbjct: 143 APLIPYPLITKEDINAIEMEEDKRDLISREISKFRDTHKKLEEEKGK-KEKERQEIEKER 201 Query: 423 VFNSDEERYRYREYAERGYERHRA-SREKE-ERHRER-RHREKEETRHKS-SRSNSRRRH 478 + ER R RE ER ER R REKE ER RER R R+++ T+ + R R R Sbjct: 202 R-ERERERERERERREREREREREREREKEKERERERERDRDRDRTKERDRDRDRERDRD 260 Query: 479 ESEEGDSHRRHKHKKSKRSKEGKEAGSEPAPEQE 512 E S R +S+ +E E E+E Sbjct: 261 RDRERSSDRNKDRΞRΞREKSRDRERERERERERE 294 Score = 126 (18.9 bits), Expect = 1.8e-04, Sum P(2) = 1.8e-04 Identities = 41/149 (27%), Positives = 66/149 (44%) Query: 375 KQWDYYARREKDRDRERDRDRERDRDRDRERERTRERERERDHSPT PSVFNSD--EE 429 K W+ R+K R+ E++ +RE +R R+ +E R +E D+ P + ++ Sbjct: 354 KNWEI-RERKKTREYEKEAEREEERRREMAKEAKRLKEFLEDYDDDRDDPKYYRGSALQK 412 Query: 430 RYRYREYAERGYERHRASREKEERHRERR HREKEETRHKSSRSNSRRRHEΞ—E 481 R R RE ER R REKEE R+ H + + + + RRR + Sbjct: 413 RLRDREKEMEADERDR-KREKEELEEIRQRLLAEGHPDPDAELQRMEQEAERRRQPQIKQ 471 Query: 482 EGDSHRRHKHKKSKRSKEGKEAGSEPAPEQE 512 E +S + K+ K K + E PEQ+ Sbjct: 472 EPESEEEEEEKQEKEEKREEPMEEEEEPEQK 502 Score = 124 (18.6 bits), Expect = 3.0e-04, Sum P(2) = 3.0e-04 Identities = 41/141 (29%), Positives = 65/141 (46%) Query: 380 YARREKDRD-RERDRDRERDRDRDRERERTRERERERDHSPTPSVFNSDEERYRYREYAE 438 Y R K+ + RER + RE +++ +RE ER RE +E + + D++R + Y Sbjct: 349 YQERLKNWEIRERKKTREYEKEAEREEERRREMAKEAKRLKE-FLEDYDDDRDDPKYYRG 407 Query: 439 RGYERHRASREKEERHRER-RHREKEETRHKSSRSNSRRRHESEEGDSHRRHKHKKSKRS 497 ++ REKE ER R REKEE R + H + + R + + +R Sbjct: 408 SALQKRLRDREKEMEADERDRKREKEELEEIRQRLLAEG-HPDPDAELQRMEQEAERRRQ 466 Query: 498 KEGKEAGSEPAPEQESTEATPAE 520 + K+ EP E+E E E Sbjct: 467 PQIKQ EPESEEEEEEKQEKE 486 Score = 121 (18.2 bits), Expect = 6.2e-04, Sum P(2) = 6.2e-04 Identities = 43/149 (28%), Positives = 67/149 (44%) Query: 364 APSWPSLVDTSKQWDYYARREKDRDR-ERDRDRERDRDRDRERERTRERERERDHSPTPS 422 AP P + T + + E RD R+ + RD + E E+ + +E+ER Sbjct: 143 APLIPYPLITKEDINAIEMEEDKRDLISREISKFRDTHKKLEEEKGK-KEKERQEIEKE- 200 Query: 423 VFNSDEERYRYREYAERGYERHRASREKEERHRERRHREKEETRHKSSRSNSRRRHESEE 482 + ER R RE R ER R RE+E + R RE+E R + R+ R R E Sbjct: 201 —RRERERERERERERRERERER-EREREREKEKERERERERDRDRD-RTKERDRDRDRE 256 Query: 483 GDSHRRHKHKKSKRSKEGKEAGSEPAPEQE 512 D R + + S R+K+ + E + ++E Sbjct: 257 RDRDR-DRERSSDRNKD-RΞRSREKSRDRE 284 Score = 105 (15.8 bits), Expect = 3.1e-02, Sum P(2) = 3.1e-02 Identities = 25/73 (34%), Positives = 33/73 (45%) Query: 4 2 8 EERYRYREYAERGYERHRASREKE-ERHRERRHREKEETRHKSSRSNSRRRHESEEGDSH 486 EE +E + E+ R RE+E ER RERR RE+E R + R E E Sbjct: 184 EEEKGKKEKERQEIEKERRERERERERERERREREREREREREREKEKERERERERDRDR 243 Query: 487 RRHKHKKSKRSKE 499 R K + R +E Sbjct: 244 DRTKERDRDRDRE 256 Score = 105 (15.8 bits), Expect = 3.1e-02, Sum P(2) = 3. le-02 Identities = 31/87 (35%), Positives = 45/87 (51%) Query: 382 RREKDRDRERDRDRERDRDRDRER-ERTRERERERDHSPTPSVFNSDEERYRYREYAERG 440 +R +DR++E + D ERDR R++E E R+R H P P D E R + AER Sbjct: 412 KRLRDREKEMEAD-ERDRKREKEELEEIRQRLLAEGH-PDP DAELQRMEQEAERR 464 Query: 441 YERHRASREKEERHRERRHREKEETRHK 468 + + +E E E +EKEE R + Sbjct: 465 -RQPQIKQEPESEEEEEEKQEKEEKREE 491 Score = 46 (6.9 bits), Expect = 1.5e-16, Sum P(2) = 1.5e-16 Identities = 13/49 (26%), Positives = 21/49 (42%) Query: 54 AENGVPKPKVTETEDDSDSDSDDDEDDVHVTIGDIKTGAPQYGSYGTAP 102 A NG +P+ +D+ D + D + G 1+ +Y S AP Sbjct: 70 ASNGNARPETVTNDDEEALDEETKRRDQMIK-GAIEVLIREYSSELNAP 117 Score = 46 (6.9 bits), Expect = 1.8e-04, Sum P(2) = 1.8e-04 Identities = 14/53 (26%), Positives = 21/53 (39%) Query: 30 ENEVERPEEENASANPPSGIEDETAENGVPKPKVTETEDDSDSDSDDDEDDVH 82 + E ER E E E E + + E E D D ++DE+D + Sbjct: 282 DRERERERERERERERERERERER-EREREREREREREKDKKRDREEDEEDAY 333 Score = 44 (6.6 bits), Expect = 2.0e-13, Sum P(2) = 2.0e-13 Identities = 13/60 (21%), Positives = 21/60 (35%) Query: 20 DEEEEWLYGDENEVERPEEENASANPPSGIEDETAENGVPKPKVTETEDDSDSDSDDDED 79 ++E + + + E ER E + E K + E E D D D + D Sbjct: 191 EKERQEIEKERRERERERERERERREREREREREREREKEKERERERERDRDRDRTKERD 250 Pedant information for DKFZphutel_17k7, frame 3 Report for DKFZphutel_17k7.3 [LENGTH] 520 [MW] 58375.30 [pi] 5.41 [HOMOL] PIR:S62454 hypothetical protein SPAC22G7.10 - fission yeast (Schizosaccharomyces pombe) 3e-18 [FUNCAT] 04.05.05 mrna processing (5'-end, 3'-end processing and mrna degradation) [S. cerevisiae, YJR093c] 2e-13 [FUNCAT] 3 300..1100 nnuucclleeaarr oorrggaanniizzaattiioonn [Ξ. cerevisiae, YJR093c] 2e-13 [PROSITE] MYRISTYL 9 [PROSITE] AMIDATION 1 [PROSITE] CK2 PHOSPHO SITE 18 [PROSITE] TYR PHOSPHO SITE 2 [PROSITE] PKC PHOSPHO SITE 12 [PROSITE] ASN GLYCOSYLATION 2 [KW] Alpha Beta [KW] LOW COMPLEXITY 35. .00 % SEQ MSAGEVERLVSELΞGGTGGDEEEEWLYGDENEVERPEEENASANPPSGIEDETAENGVPK SEG xxxxxxxxxx PRD cccchhhhhhhhcccccccccccccccccccccccccccccccccccccccccccccccc SEQ PKVTETEDDSDSDSDDDEDDVHVTIGDIKTGAPQYGSYGTAPVNLNIKTGGRVYGTTGTK SEG ... xxxxxxxxxxxxxxxxx PRD cceeeecccccccccccccceeeeeccccccccccccccccceeeeeecccceeeccccc SEQ VKGVDLDAPGSINGVPLLEVDLDSFEDKPWRKPGADLSDYFNYGFNEDTWKAYCEKQKRI SEG PRD ceeeccccccccccceeeeccccccccccccccccccccccccccccchhhhhhhhhhhh SEQ RMGLEVIPVTSTTNKITVQQGRTGNSEKETALPSTKAEFTSPPSLFKTGLPPΞRRLPGAI SEG PRD hhhheeeeeccccceeeeeeecccccccccccccceeeeccccceeeecccccccccccc SEQ DVIGQTITISRVEGRRRANENSNIQVLSERSATEVDNNFSKPPPFFPPGAPPTHLPPPPF SEG xxxxxxxxxxxxxxxxxxx PRD ccccceeeeeecccccccccccceeecccccccccccccccccccccccccccccccccc SEQ LPPPPTVSTAPPLIPPPGFPPPPGAPPPSLIPTIESGHSSGYDSRSARAFPYGNVAFPHL SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccceeeccc SEQ PGSAPSWPSLVDTSKQWDYYARREKDRDRERDRDRERDRDRDRERERTRERERERDHSPT SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx .... PRD ccccccccceeeccccchhhhhhhhhhccccccccccccccchhhhhhhhhhhhcccccc SEQ PSVFNSDEERYRYREYAERGYERHRASREKEERHRERRHREKEETRHKSSRSNSRRRHES SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD cccccccchhhhhhhhhhhhchhhhhhhhhhhhhhhhhhhhhhhhhhhcccccccccccc SEQ EEGDSHRRHKHKKSKRSKEGKEAGΞEPAPEQESTEATPAE SEG xx .. xxxxxxxxxxxxxx PRD cccccccccccccccccccccccccccccccccccccccc Prosite for DKFZphutel_17k7.3 PS00001 40->44 ASN_GLYCOSYLATION PDOC00001 PS00001 278->282 ASN_GLYCOSYLATION PDOC00001 PS00005 169->172 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 193->196 PKC_PHOSPHO_SITE PDOC00005 PS00005 206->209 PKC_PHOSPHO_SITE PDOC00005 PS00005 214->217 PKC_PHOSPHO_SITE PDOC00005 PS00005 233->236 PKC_PHOSPHO_SITE PDOC00005 PS00005 268->271 PKC_PHOSPHO_SITE PDOC00005 PS00005 346->349 PKC_PHOSPHO_SITE PDOC00005 PS00005 373->376 PKC_PHOSPHO_SITE PDOC00005 PS00005 469->472 PKC_PHOSPHO_SITE PDOC00005 PS00005 474->477 PKC_PHOSPHO_SITE PDOC00005 PS00005 485->488 PKC_PHOSPHO_SITE PDOC00005 PS00005 494->497 PKC_PHOSPHO_SITE PDOC00005 PS00006 2->6 CK2_PHOSPHO_SITE PDOC00006 PS00006 17->21 CK2_PHOSPHO_SITE PDOC00006 PS00006 47->51 CK2_PHOSPHO_SITE PDOC00006 PS00006 64->68 CK2_PHOSPHO_SITE PDOC00006 PS00006 66->70 CK2_PHOSPHO_ΞITE PDOC00006 PS00006 70->74 CK2_PHOSPHO_SITE PDOC00006 PS00006 72->76 CK2_PHOSPHO_SITE PDOC00006 PS00006 74->78 CK2_PHOSPHO_SITE PDOC00006 PS00006 84->88 CK2_PHOSPHO_SITE PDOC00006 PS00006 144->148 CK2_PHOSPHO_SITE PDOC00006 PS00006 206->210 CK2_PHOSPHO_SITE PDOC00006 PS00006 215->219 CK2_PHOSPHO_SITE PDOC00006 PS00006 250->254 CK2_PHOSPHO_SITE PDOC00006 PS00006 271->275 CK2_PHOSPHO_SITE PDOC00006 PS00006 273->277 CK2_PHOSPHO_SITE PDOC00006 PS00006 340->344 CK2_PHOSPHO_SITE PDOC00006 PS00006 369->373 CK2_PHOSPHO_SITE PDOC00006 PS00006 426->430 CK2_PHOSPHO_SITE PDOC00006 PS00007 434->442 TYR_PHOSPHO_SITE PDOC00007 PS00007 152->161 TYR_PHOSPHO_SITE PDOC00007 PS00008 15->21 MYRISTYL PDOC00008 PS00008 96->102 MYRISTYL PDOC00008 PS00008 115->121 MYRISTYL PDOC00008 PS00008 130->136 MYRISTYL PDOC00008 PS00008 154->160 MYRISTYL PDOC00008 PS00008 229->235 MYRISTYL PDOC00008 PS00008 244->250 MYRISTYL PDOC00008 PS00008 289->295 MYRISTYL PDOC00008 PS00008 362->368 MYRISTYL PDOC00008 PS00009 253->257 AMIDATION PDOC00009 (No Pfam data available for DKFZphutel_17k7.3) DKFZphutel_18cl2 group: uterus derived DKFZphutel_18cl2 encodes a novel 378 amino acid protein nearly identical to human WUGSC : H_DJ0872F07.1 protein. The novel protein has an additional N-terminal domain, which is not present in WUGSC : H_DJ0872F07.1. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of uterus-specific genes . nearly identical to human WUGSC : H_DJ0872F07.1 protein on genomic level encoded by AC004537, 10 exons the predicted protein sequence AC004537_1 is only partialy o.k. first exon wasn't predicted there are additional exons predicted (BLASTX/EST-BLAST shows that the cDNA is only party spliced) intron -1216-3540//-3577-5059 Sequenced by AGOWA Locus: map="7q31" Insert length: 6005 bp Poly A stretch at pos. 5980, polyadenylation signal at pos. 5968 1 AGCGGGTGCT GCTAGCGGAG GCGCCATATT GGAGGGGACA AAACTCCGGC 51 GACAGCGAGT GACACAAATA AACCCCTGGA CCCCCTTGTT CCCTCAGCTC 101 TAAGGGCCGC GATGTTGTAC CTAGAAGACT ATCTGGAAAT GATTGAGCAG 151 CTTCCTATGG ATCTGCGGGA CCGCTTCACG GAAATGCGCG AGATGGACCT 201 GCAGGTGCAG AATGCAATGG ATCAACTAGA ACAAAGAGTC AGTGAATTCT 251 TTATGAATGC AAAGAAAAAT AAACCTGAGT GGAGGGAAGA GCAAATGGCA 301 TCCATCAAAA AAGACTACTA TAAAGCTTTG GAAGATGCAG ATGAGAAGGT 351 TCAGTTGGCA AACCAGATAT ATGACTTGGT AGATCGACAC TTGAGAAAGC 401 TGGATCAGGA ACTGGCTAAG TTTAAAATGG AGCTGGAAGC TGATAATGCT 451 GGAATTACAG AAATATTAGA GAGGCGATCT TTGGAATTAG ACACTCCTTC 501 ACAGCCAGTG AACAATCACC ATGCTCATTC ACATACTCCA GTGGAAAAAA 551 GGAAATATAA TCCAACTTCT CACCATACGA CAACAGATCA TATTCCTGAA 601 AAGAAATTTA AATCTGAAGC TCTTCTATCC ACCCTTACGT CAGATGCCTC 651 TAAGGAAAAT ACACTAGGTT GTCGAAATAA TAATTCCACA GCCTCTTCTA 701 ACAATGCCTA CAATGTGAAT TCCTCCCAAC CTCTGGGATC CTATAACATT 751 GGCTCGTTAT CTTCAGGAAC TGGTGCAGGG GCAATTACCA TGGCAGCTGC 801 TCAAGCAGTT CAGGCTACAG CTCAGATGAA GGAGGGACGA AGAACATCAA 851 GTTTAAAAGC CAGTTATGAA GCATTTAAGA ATAATGACTT TCAGTTGGGA 901 AAAGAATTTT CAATGGCCAG GGAAACAGTT GGCTATTCAT CATCTTCGGC 951 ACTTATGACA ACATTAACAC AGAATGCCAG TTCATCAGCA GCCGACTCAC 1001 GGAGTGGTCG AAAGAGCAAA AACAACAACA AGTCTTCAAG CCAGCAGTCA 1051 TCATCTTCCT CCTCCTCTTC TTCCTTATCA TCGTGTTCTT CATCATCAAC 1101 TGTTGTACAA GAAATCTCTC AACAAACAAC TGTAGTGCCA GAATCTGATT 1151 CAAATAGTCA GGTTGATTGG ACTTACGACC CAAATGAACC TCGATACTGC 1201 ATTTGTAATC AGGTAAAAGT CTGTTATATC TATAAAAGTA TAATCTGAAT 1251 AAACTAGAAG GAAGAGAACT ATTTCATTTT TAAGCACTTT TTTAAACTCA 1301 CTTAAAATAC CTTTGCTTTA TTTGTATACT TTTCTCCCCC TTCTTACAAA 1351 AGTGACATTT GCTGTAAATA CTGAGTATAA AGAAAAATGT TACCCATAAT 1401 CCTAGCCCTC AGATACAACC TGTAACTAAA CATTTTTGGT ATACCACTAC 1451 CATATACCTC ATGTGCACAT TGGCTGCCTT AATAAAATAC AACAGACTGG 1501 GTAGCTTAAA CAACAGAAAA TAATTTTCTC ACAGGTATGA AGGCTGGGAA 1551 GTCCAAGATC AAGGTGTCCA CTGACTCAGT TCTGGAGGAG GGCTCCCTTC 1601 CTAGATGGAG ACTGCTGCCT TCTCACCGGG TCCTCACATG ATAGAGGGAG 1651 AAAGAGTGTG CTCTGGTGTC TTTTCTTATA AGGGCACCAG CCTTGTCAGA 1701 GTAGGACCCC ACTCTATGAC CTCATTTAAC CTTTACCACC TCCTCACAGG 1751 CCCTGTTTCC AATTATAGTC ACGTTGGGGG TTAGGGCTTC AACATATGAT 1801 TTTGAGACAT AAGCTTGCAT TTCATAACAC GTGTCTATGC AGATTTGCAC 1851 ATGCATGTGT GTATAAGTTT GTCAGTAGGA ACCACAGTGT ATACTTTCTT 1901 GTTACTGGCT TTTTTCTCTA AATCAGGTAT ACCGAACATG ATTTTTCTTT 1951 AAGATCATAT TTTTAATTTT CACATAGTTA TCTCTTATGC CATCCAGTGT 2001 AGTTTTCTTA ACCAATACCT AGCTATAGAT TATATTAGTG GTTTTAATTT 2051 GTTTGAAATT AGGGATAATA TTACGATAGG CATTTTTTAA ATGTAATCCA 2101 TTTTATACAT CTAATTTCTT GGATAATCTT TTAGAAATAA AATTAGGCTG 2151 TAAATATTTG ACAGACACCA AAATATATTT TCTAGAAATT TATTACCAAA 2201 AATTAATAAA CATACCGGTT TACTAAACCC TGTCCAACAC TGGATATTAT 2251 TTTCTTTTAA AAACTAAGTA CCAATTTGGT AGTTTTATAT TATGATTGTT 2301 TTAAATACAC TAGTATTATT GAAGTTGGAC ATTTTTTGAC CATTTTTGTT 2351 TTTTACATTA TGAATCGACT CCTAATGGTG TCGGCTGATT TTTCTATTGT 2401 TTTTGTTATG TACTCTAAAT ATTTGCTTGA TTTAGTTTTT TAAAAATAAT 2451 TCTAAAATTT TAATTTTATG TAGTTATGAC TGTTAATTTT TTTTTATGAA 2501 GCAAGCCATG GATTATATAC TTAGAAGGGC TTTCTCTTTG GCTCTTCTTT 2551 CTACAAAAAA TTGTCTTGTA TAATATTTTC TCCTAGTTTT TATATGGTTT 2601 TGTCTAGTTC TTTGCATGCT TCAGTTTCTT CACATTTAAG ACTTAGTCTA 2651 TCAGCAGATT ATTGTGTCTA ACAGTATGAG TTGCCAGTCT GATTTTTAAA 2701 AATTTTAACA ATTTGTTAGC TGTTCCACTA TCACCCGATA AACATTTTTC 2751 AGTACAAATG ATAGAAAAGC ATATCCTGTA TCCTGACAAC AAAAGTAGAT 2801 TACTTGCAAA AGAACAAAAT CAGACTGAAC CTAGAGTTTT CCTCTGTAAC 2851 ACTAAAAAAC TAGAAGGTGA TGGAATATGT CTGTAGAGCT TTCAGGGAAA 2901 AATTAAGAGC CCCCAAAAAC TTGATATTCA GAGAAGTTAT TTCTCTGCAT 2951 AGGACCATGT AAATATATTT TCACTCATGC AGAGAATCAG AAGATATGCC 3001 ATCTAGTTAA TCCTGTCTGA AAAATTATTC AATCCACTGA GAACTTCAGT 3051 GAACTCAAGA ATTAGCAAGT TATGCCCTAA AGTGCTGGTG ATGAAGAGCA 3101 AAAGAAAAAT GAGAAAGGAC ATAAAATAGA TAAGTTTAGA AGTTTCAAGG 3151 AAGGAGACTA TTAATTGCAA AAATATATAT GACCTAATGT GACCCAAGAA 3201 GTAAAAACTT TCAGTAAGTA AATAATCAAG AAAGGAACTT AAAATTTTTA 3251 CAATAAGAAC TACCCAGAAA GATGACTCCT TCATCCGGGT GATTTATATG 3301 TCAAGTTCTT CCAGACTTCT GAAGGGCAGA TAATTCCTGT GCATTTCTTC 3351 CCACCCTTGC CCCACCCTGC CCAAAAGAGT ATTTCAGGAA AAAATTATTA 3401 TACCTTGATT CTCAATGTAA TTGTATATTC AGTGTATTTC CCTTTATTTT 3451 CCAGCAGTAT CATACATAAA CAGTTAATTG GTATCTAGGT GTTTGTTACA 3501 TAGTCATAAT AAAGACATTT AATTTTTTTT AACTAGGTAT CTTATGGTGA 3551 GATGGTGGGA TGTGATAACC AAGATGTAAG TATTACATTT TTCTATTTAG 3601 GAATGAAAAA AATCACAGGT TGTTATTACT TGAATATTTG TCTTATTTGC 3651 TGTATGGTTT GGTCTAAGAA AACAGGTTTG CAGGTATATT AGTTATGTTA 3701 TGCTAATGCT AGAATATTCC TCTTCAAAAT AGGGTAGTGT CCCTTAATGT 3751 GTTCCCTATT TTAATTTTTA AAGCTAATTT TATGGTTTTA TGTGCAGATT 3801 GTCTCAGAAG TGTTATGTTG TATGAAAATT ATAAATACCC TCCTTTCCCT 3851 TTACTAAAAA ATACTGTGTT TACTAGAATC CAGTTCATTT ATCACATTGA 3901 AGAAATGGAA TTTTAAAACA ATTCATTCTT TCAGGCTGCA CCGTGCTAAA 3951 GTGAAGGGTG GGATAATTGA GGATCTAATG TGAGATTATC TTCCTCTCAT 4001 GAGTATAATA TTTTTTCCTG TACTCTGCAG GTGTCAGCTG ATAAGAGCCA 4051 CCCCTGATCT AAAAAGTAAA GGAAATTTGA AAGGAAGGAA TTCTTGGTTT 4101 TTAGGAGACT TAATTTTAGT TAGAGATACG TTTTTTATTC AATACTGAGA 4151 ATATTGTTGT CTAGTAATTT TGACTCCCTC CTTATTTAGT AGTGACAGGA 4201 TCCTAAGATT AACAAGAGTT TTAAATTTGT AAAACAATCT GAAGATTGAG 4251 GGAGCTGGCT AGGTGCATTA AAATGTGTAC TTTTCCTAGA CCTGATAGGG 4301 TTACAGCAAC ATGCTCACGT AGATTGGGAC AGAGCCTCCT TCTGTTTCCC 4351 TGTCTAGAAT CCCTTGTAGG CTGTTTGTGG TTGTTGCAAA AACAATATTG 4401 CCCAACCATT TCAAGAACAT CACTGTAAAC TCTTCTGGGG CAGTTAGTGA 4451 AAATGATGAA TGAGATTTCT ATGAGTACCA GCATCATGCT TCTCTGATTC 4501 TTCTTATTCC CAGTTGTGCT CTTCTGAGTG CTAAGACTTT CATGAAAGAG 4551 TTTTCTGCTT AATATGTTTC AAAGAGGAAT AATTTTTCTC TACATTTCAA 4601 GGAATAGAAA CACCCACGTA GGAAATGCAG GGCATAAGAC ATAAATTAAT 4651 GTCTTTAATT ACAATCAGCT TATTCTACTT TATGAGACAG CAAATAAGGC 4701 TGACTATTAA ATAAAATCTT AAGTTATATT TACCTTCTAC ATAGAAGATT 4751 CATCCCACTT CTTTTTGCCC TTGAAAGCTG AAAACTAGTG AATTTTCATT 4801 CATTAGGATG AGGGGACTAG ATTACATGGA CCTCAGGATT CTTGAAGATG 4851 CATAATTTTT CTGTGCCTTC ATTTCCTCAT TCCTGAAGCT TATCATTTAG 4901 TCTAAATGAT GTCTAAATAA TCTAGATCTA AAAATTCTGA TGTCACACAT 4951 CTAATTATTG TTAAATTAAA TGGATTATTC AGTCTCCTGA GCATATTTTA 5001 ATATACTCTC TTGTCTTCAG AAGTACTGAA AACTTGTTTT TTGCAATTTT 5051 GCTTTCTAGT GCCCTATAGA ATGGTTCCAT TATGGCTGCG TTGGATTGAC 5101 AGAGGCACCA AAAGGCAAAT GGTACTGTCC ACAGTGCACT GCTGCAATGA 5151 AGAGAAGAGG CAGCAGACAC AAATAAAGGT GGTCCTTTTG TTTGATGAAG 5201 AAATAAACTT CAGCTGAAGA TTTTATATAG GACTTTAAAA AGAAGAGAAG 5251 AGAAAGAAGA AACAATGCAT TTCCAGGCAA CCACTTAAAG GATTTACATA 5301 GACAATCCTA TAAGATCTTG AACTTGAATT TTATGGGTTG TATTTTAATA 5351 ATGTAAGTAA ATTATTTATG CACTCCTGGT GTGCTATGAA TATTATTCCA 5401 GTTAGCCTTG GATTATTTCA GTGGCCAACA TATGCAGACA TTTGTACTCC 5451 TCAACCATTT TCTCAAAGTA ATGGGCATTC TATGATTTAG ACTTCAAGGA 5501 ATTCCAATGA TGAAGATTTT AAGGAAAGTA TTTTATATTC AACAGGTATA 5551 TTCTGCTGCA TGTACTGTAC TCCAGAGCTG TTATGTAACA CTGTATATAA 5601 ATGGTTGCAA AAAAAAAAAA AAGTCAGTGC TTCTAAAAAG AATTTAAGAT 5651 AATGGTTTTT AAAATGCCTT TATAATAAGC TTTGTTTCTT TGTGAAACTA 5701 ATTCAGCAGG CTGAAGGAAA TGGTTCATGT GATAATGTGG GCTGGTATCC 5751 TCTAGAGTAC CTGGGTACAT AAACAGAAAC TCCTGTAGGT AAAAAGTAAT 5801 TTGTGCCATT AGTCTTTCTA TGTTTCTGCA TCCAGATAGA GTGCAGTTCA 5851 TGAGGGAGGG GGCGGGGGAC TGAAGGGGAA AGGGCGTTAA AGTGATACAT 5901 TTTTATACCA AATGTGTTTA TTTTTTTGTG CAAGTAATCC TTAAAATTGC 5951 AATTGTATTA GGTGTTAAAA TAAAGTTTTT AAAAAATTAA AAAAAAAAAA 6001 AAAAA BLAST Results Entry HSG20547 from database EMBL: HSG20547| human STS A005W09. Length = 154 Minus Strand HSPs: Score = 770 (115.5 bits), Expect = 2.9e-26, P = 2.9e-26 Identities = 154/154 (100%) Medline entries 98101645: The candidate tumour suppressor p33INGl cooperates with p53 in cell growth control. Peptide information for frame 1 ORF from 112 bp to 1245 bp; peptide length: 378 Category: similarity to known protein 1 MLYLEDYLEM IEQLPMDLRD RFTEMREMDL QVQNAMDQLE QRVSEFFMNA 51 KKNKPEWREE QMASIKKDYY KALEDADEKV QLANQIYDLV DRHLRKLDQE 101 LAKFKMELEA DNAGITEILE RRSLELDTPS QPVNNHHAHS HTPVEKRKYN 151 PTSHHTTTDH IPEKKFKΞEA LLSTLTSDAS KENTLGCRNN NSTASSNNAY 201 NVNSSQPLGS YNIGSLSSGT GAGAITMAAA QAVQATAQMK EGRRTSSLKA 251 SYEAFKNNDF QLGKEFSMAR ETVGYSSSSA LMTTLTQNAS SSAADSRSGR 301 KSKNNNKSSS QQSSSSSSSS SLSSCSSSST VVQEISQQTT VVPESDSNSQ 351 VDWTYDPNEP RYCICNQVKV CYIYKSII BLASTP hits Entry AF044076_1 from database TREMBL: "INGl"; product: "candidate tumor suppressor p33INGl"; Homo sapiens candidate tumor suppressor p33INGl (INGl) mRNA, complete eds. Homo sapiens (human) Length = 279 Score = 162 (57.0 bits), Expect = l.le-09, P = l.le-09 Identities = 48/183 (26%), Positives = 92/183 (50%) Entry AC004537_1 from database TREMBL: gene: "WUGSC :H_DJ0872F07.1"; Homo sapiens PAC clone DJ0872F07 from 7q31, complete sequence. Score = 1814, P = 3.7e-187, identities = 358/358, positives = 358/356 Entry CEY51H1A_1 from database TREMBL: gene: "Y51H1A.4"; Caenorhabditis elegans cosmid Y51H1A Score = 213, P = 3.7e-15, identities = 37/123, positives = 82/123 Alert BLASTP hits for DKFZphutel_18cl2, frame 1 No Alert BLASTP hits found Pedant information for DKFZphutel_18cl2, frame 1 Report for DKFZphutel_18cl2.1 [LENGTH] 378 [MW] 42275.72 [pi] 5.72 [HOMOL] TREMBL:AC004537_1 gene: "WUGSC :H_DJ0872F07.1"; Homo sapiens PAC clone DJ0872F07 from 7q31, complete sequence, le-157 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YHR090c] 8e-05 [FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YNL097c] 2e-04 [PROSITE] MYRISTYL 3 [PROSITE] AMIDATION 2 [PROSITE] CAMP_PHOSPHO_SITE 1 [PROSITE] CK2_PHOSPHO_SITE 4 [PROSITE] PROKAR_LIPOPROTEIN 1 [PROSITE] GLYCOSAMINOGLYCAN 1 [PROSITE] PKC_PHOSPHO_SITE 3 [PROSITE] ASN_GLYCOSYLATION 5 [KW] All_Alpha [KW] LOW COMPLEXITY 20.63 % [KW] COILED COIL 7.94 % SEQ MLYLEDYLEMIEQLPMDLRDRFTEMREMDLQVQNAMDQLEQRVSEFFMNAKKNKPEWREE SEG PRD ccchhhhhhhhhhhccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccchhhhh COILS SEQ QMASIKKDYYKALEDADEKVQLANQIYDLVDRHLRKLDQELAKFKMELEADNAGITEILE SEG PRD hhhhhhhhhhhhhhhchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccchhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCC SEQ RRSLELDTPSQPVNNHHAHSHTPVEKRKYNPTSHHTTTDHIPEKKFKSEALLSTLTSDAS SEG PRD hhccccccccccccccccccccccccccccccccccccccccchhhhhhhhhhhhhcccc COILS SEQ KENTLGCRNNNSTASSNNAYNVNSSQPLGΞYNIGSLSSGTGAGAITMAAAQAVQATAQMK SEG xxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxx.. PRD cccccccccccccccccccccccccccccccccccccccccchhhhhhhhhhhhhhhhhh COILS SEQ EGRRTSSLKASYEAFKNNDFQLGKEFSMARETVGYSSSSALMTTLTQNASSSAADSRSGR SEG xxxxxxxxxxxx PRD hccccccccchhhhhhccccccccccccccccccccccceeeeecccccccccccccccc COILS SEQ KSKNNNKSSSQQSSSSSSSSSLSSCSSSSTVVQEISQQTTVVPESDSNSQVDWTYDPNEP SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD ccccccccccccccccccccceeecccccccccccccccccccccccccceeeecccccc COILS SEQ RYCICNQVKVCYIYKSII SEG PRD eeeeceeeeeeeeeeccc COILS Prosite for DKFZphutel_18cl2.1 PS00001 190->194 ASN_GLYCOSYLATION PDOC00001 PS00001 191->195 ASN_GLYCOSYLATION PDOC00001 PS00001 203->207 ASN_GLYCOSYLATION PDOC00001 PS00001 288->292 ASN_GLYCOSYLATION PDOC00001 PΞ00001 306->310 ASN_GLYCOSYLATION PDOC00001 PS00002 218->222 GLYCOSAMINOGLYCAN PDOC00002 PS00004 243->247 CAMP_PHOSPHO_SITE PDOC00004 PS00005 64->67 PKC_PHOSPHO_SITE PDOC00005 PS00005 247->250 PKC_PHOSPHO_SITE PDOC00005 PS00005 298->301 PKC_PHOSPHO_SITE PDOC00005 PΞ00006 142->146 CK2_PHOSPHO_SITE PDOC00006 PS00006 156->160 CK2_PHOSPHO_SITE PDOC00006 PS00006 292->296 CK2_PHOΞPHO_SITE PDOC00006 PS00006 349->353 CK2_PHOSPHO_SITE PDOC00006 PS00008 186->192 MYRISTYL PDOC00008 PS00008 214->220 MYRISTYL PDOC00008 PS00008 219->225 MYRISTYL PDOC00008 PS00009 241->245 AMIDATION PDOC00009 PS00009 298->302 AMIDATION PDOC00009 PS00013 315->326 PROKAR LIPOPROTEIN PDOC00013 (No Pfam data available for DKFZphutel 18cl2.1) DKFZphutel_18ιl9 group: transcription factors DKFZphutel_18ιl9 encodes a novel 759 ammo acid protein with similarity to the SREBP-2 mutant sterol regulatory element binding protem-2 of Cricetulus gπseus. The SREBP-2 protein is embedded in the membranes of the nucleus and endoplasmic reticulum. In cholesterol-depleted cells the proteins are cleaved to release soluble NH2-termmal fragments that enter the nucleus and activate genes encoding the low density lipoprotein receptor and enzymes of cholesterol synthesis. The new protein is a putative transcription factor capable of protein-protein interaction via a lim domain and additionally shows similarity to the common sunflower transcription factor SF3. The new protein can find application in modulating/blocking the expression of genes involved m lipid metabolism. similarity to transcription factor ΞF3 complete cDNA, complete eds, EST hits strong similarity to mutated SREBP-2 of hamster, similarity is not to SREP-2 part of protein but to the unknown part of the fusion protein Sequenced by AGOWA Locus: /map=12 Insert length: 3664 bp Poly A stretch at pos. 3647, polyadenylation signal at pos. 3636 1 GCGCTAGGTA GAGCGCCGGG ACCTGTGACA GGGCTGGTAG CAGCGCAGAG 51 GAAAGGCGGC TTTTAGCCAG GTATTTCAGT GTCTGTAGAC AAGATGGAAT 101 CATCTCCATT TAATAGACGG CAATGGACCT CACTATCATT GAGGGTAACA 151 GCCAAAGAAC TTTCTCTTGT CAACAAGAAC AAGTCATCGG CTATTGTGGA 201 AATATTCTCC AAGTACCAGA AAGCAGCTGA AGAAACAAAC ATGGAGAAGA 251 AGAGAAGTAA CACCGAAAAT CTCTCCCAGC ACTTTAGAAA GGGGACCCTG 301 ACTGTGTTAA AGAAGAAGTG GGAGAACCCA GGGCTGGGAG CAGAGTCTCA 351 CACAGACTCT CTACGGAACA GCAGCACTGA GATTAGGCAC AGAGCAGACC 401 ATCCTCCTGC TGAAGTGACA AGCCACGCTG CTTCTGGAGC CAAAGCTGAC 451 CAAGAAGAAC AAATCCACCC CAGATCTAGA CTCAGGTCAC CTCCTGAAGC 501 CCTCGTTCAG GGTCGATATC CCCACATCAA GGACGGTGAG GATCTTAAAG 551 ACCACTCAAC AGAAAGTAAA AAAATGGAAA ATTGTCTAGG AGAATCCAGG 601 CATGAAGTAG AAAAATCAGA AATCAGTGAA AACACAGATG CTTCGGGCAA 651 AATAGAGAAA TATAATGTTC CGCTGAACAG GCTTAAGATG ATGTTTGAGA 701 AAGGTGAACC AACTCAAACT AAGATTCTCC GGGCCCAAAG CCGAAGTGCA 751 AGTGGAAGGA AGATCTCTGA AAACAGCTAT TCTCTAGATG ACCTGGAAAT 801 AGGCCCAGGT CAGTTGTCAT CTTCTACATT TGACTCGGAG AAAAATGAGA 851 GTAGACGAAA TCTGGAACTT CCACGCCTCT CAGAAACCTC TATAAAGGAT 901 CGAATGGCCA AGTACCAGGC AGCTGTGTCC AAACAAAGCA GCTCAACCAA 951 CTATACAAAT GAGCTGAAAG CCAGTGGTGG CGAAATCAAA ATTCATAAAA 1001 TGGAGCAAAA GGAGAATGTG CCCCCAGGTC CTGAGGTCTG CATCACCCAT 1051 CAGGAAGGGG AAAAGATTTC TGCAAATGAG AATAGCCTGG CAGTCCGTTC 1101 CACCCCTGCC GAAGATGACT CCCGTGACTC CCAGGTTAAG AGTGAGGTTC 1151 AACAGCCTGT CCATCCCAAG CCACTAAGTC CAGATTCCAG AGCCTCCAGT 1201 CTTTCTGAAA GTTCTCCTCC CAAAGCAATG AAGAAGTTTC AGGCACCTGC 1251 AAGAGAGACC TGCGTGGAAT GTCAGAAGAC AGTCTATCCA ATGGAGCGTC 1301 TCTTGGCCAA CCAGCAGGTG TTTCACATCA GCTGCTTCCG TTGCTCCTAT 1351 TGCAACAACA AACTCAGTCT AGGAACATAT GCATCTTTAC ATGGAAGAAT 1401 CTATTGTAAG CCTCACTTCA ATCAACTCTT TAAATCTAAG GGCAACTATG 1451 ATGAAGGCTT TGGGCACAGA CCACACAAGG ATCTATGGGC AAGCAAAAAT 1501 GAAAACGAAG AGATTTTGGA GAGACCAGCC CAGCTTGCAA ATGCAAGGGA 1551 GACCCCTCAC AGCCCAGGGG TAGAAGATGC CCCTATTGCT AAGGTGGGTG 1601 TCCTGGCTGC AAGTATGGAA GCCAAGGCCT CCTCTCAGCA GGAGAAGGAA 1651 GACAAGCCAG CTGAAACCAA GAAGCTGAGG ATCGCCTGGC CACCCCCCAC 1701 TGAACTTGGA AGTTCAGGAA GTGCCTTGGA GGAAGGGATC AAAATGTCAA 1751 AGCCCAAATG GCCTCCTGAA GACGAAATCA GCAAGCCCGA AGTTCCTGAG 1801 GATGTCGATC TAGATCTGAA GAAGCTAAGA CGATCTTCTT CACTGAAGGA 1851 AAGAAGCCGC CCATTCACTG TAGCAGCTTC ATTTCAAAGC ACCTCTGTCA 1901 AGAGCCCAAA AACTGTGTCC CCACCTATCA GGAAAGGCTG GAGCATGTCA 1951 GAGCAGAGTG AAGAGTCTGT GGGTGGAAGA GTTGCAGAAA GGAAACAAGT 2001 GGAAAATGCC AAGGCTTCTA AGAAGAATGG GAATGTGGGA AAAACAACCT 2051 GGCAAAACAA AGAATCTAAA GGAGAGACAG GGAAGAGAAG TAAGGAAGGT 2101 CATAGTTTGG AGATGGAGAA TGAGAATCTT GTAGAAAATG GTGCAGACTC 2151 CGATGAAGAT GATAACAGCT TCCTCAAACA ACAATCTCCA CAAGAACCCA 2201 AGTCTCTGAA TTGGTCGAGT TTTGTAGACA ACACCTTTGC TGAAGAATTC 2251 ACTACTCAGA ATCAGAAATC CCAGGATGTG GAACTCTGGG AGGGAGAAGT 2301 GGTCAAAGAG CTCTCTGTGG AAGAACAGAT AAAGAGAAAT CGGTATTATG 2351 ATGAGGATGA GGATGAAGAG TGACAAATTG CAATGATGCT GGGCCTTAAA 2401 TTCATGTTAG TGTTAGCGAG CCACTGCCCT TTGTCAAAAT GTGATGCACA 2451 TAAGCAGGTA TCCCAGCATG AAATGTAATT TACTTGGAAG TAACTTTGGA 2501 AAAGAATTCC TTCTTAAAAT CAAAAACAAA ACAAAAAAAC ACAAAAAACA 2551 CATTCTAAAT ACTAGAGATA ACTTTACTTA AATTCTTCAT TTTAGCAGTG 2601 ATGATATGCG TAAGTGCTGT AAGGCTTGTA ACTGGGGAAA TATTCCACCT 2651 GATAATAGCC CAGATTCTAC TGTATTCCCA AAAGGCAATA TTAAGGTAGA 2701 TAGATGATTA GTAGTATATT GTTACACACT ATTTTGGAAT TAGAGAACAT 2751 ACAGAAGGAA TTTAGGGGCT TAAACATTAC GACTGAATGC ACTTTAGTAT 2801 AAAGGGCACA GTTTGTATAT TTTTAAATGA ATACCAATTT AATTTTTTAG 2851 TATTTACCTG TTAAGAGATT ATTTAGTCTT TAAATTTTTT AGGTTAATTT 2901 TCTTGCTGTG ATATATATGA GGAATTTACT ACTTTATGTC CTGCTCTCTA 2951 AACTACATCC TGAACTCGAC GTCCTGAGGT ATAATACAAC AGAGCACTTT 3001 TTGAGGCAAT TGAAAAACCA ACCTACACTC TTCGGTGCTT AGAGAGATCT 3051 GCTGTCTCCC AAATAAGCTT TTGTATCTGC CAGTGAATTT ACTGTACTCC 3101 AAATGATTGC TTTCTTTTCT GGTGATATCT GTGCTTCTCA TAATTACTGA 3151 AAGCTGCAAT ATTTTAGTAA TACCTTCGGG ATCACTGTCC CCCATCTTCC 3201 GTGTTAGAGC AAAGTGAAGA GTTTAAAGGA GGAAGAAGAA AGAACTGTCT 3251 TACACCACTT GAGCTCAGAC CTCTAAACCC TGTATTTCCC TTATGATGTC 3301 CCCTTTTTGA GACACTAATT TTTAAATACT TACTAGCTCT GAAATATATT 3351 GATTTTTATC ACAGTATTCT CAGGGTGAAA TTAAACCAAC TATAGGCCTT 3401 TTTCTTGGGA TGATTTTCTA GTCTTAAGGT TTGGGGACAT TATAAACTTG 3451 AGTACATTTG TTGTACACAG TTGATATTCC AAATTGTATG GATGGGAGGG 3501 AGAGGTGTCT TAAGCTGTAG GCTTTTCTTT GTACTGCATT TATAGAGATT 3551 TAGCTTTAAT ATTTTTTAGA GATGTAAAAC ATTCTGCTTT CTTAGTCTTA 3601 CCTAGTCTGA AACATTTTTA TTCAATAAAG ATTTTAATTA AAATTTGAAA 3651 AAAAAAAAAA AAAA BLAST Results Entry HS512217 from database EMBL: human STS SHGC-14654. Length = 250 Minus Strand HSPs: Score = 1202 (180.3 bits), Expect = 1.8e-46, P = 1.8e-46 Identities = 242/244 (99%) Medline entries 95263566: Three different rearrangements in a single intron truncate sterol regulatory element binding proteιn-2 and produce sterol-resistant phenotype in three cell lines. Role of introns in protein evolution. 93258417: Characterization of a pollen-specific cDNA from sunflower encoding a zinc finger protein. Peptide information for frame 1 ORF from 94 bp to 2370 bp; peptide length: 759 Category: similarity to known protein 1 MESSPFNRRQ WTSLSLRVTA KELSLVNKNK SSAIVEIFSK YQKAAEETNM 51 EKKRSNTENL SQHFRKGTLT VLKKKWENPG LGAESHTDSL RNSSTEIRHR 101 ADHPPAEVTS HAASGAKADQ EEQIHPRSRL RSPPEALVQG RYPHIKDGED 151 LKDHSTESKK MENCLGESRH EVEKSEISEN TDASGKIEKY NVPLNRLKMM 201 FEKGEPTQTK ILRAQSRSAS GRKISENSYS LDDLEIGPGQ LSSSTFDSEK 251 NESRRNLELP RLSETSIKDR MAKYQAAVSK QSSSTNYTNE LKASGGEIKI 301 HKMEQKENVP PGPEVCITHQ EGEKISANEN SLAVRSTPAE DDSRDSQVKS 351 EVQQPVHPKP LSPDSRASSL ΞESSPPKAMK KFQAPARETC VECQKTVYPM 401 ERLLANQQVF HISCFRCSYC NNKLSLGTYA SLHGRIYCKP HFNQLFKSKG 451 NYDEGFGHRP HKDLWASKNE NEEILERPAQ LANARETPHS PGVEDAPIAK 501 VGVLAASMEA KASSQQEKED KPAETKKLRI AWPPPTELGS SGSALEEGIK 551 MSKPKWPPED EISKPEVPED VDLDLKKLRR SSSLKERSRP FTVAASFQST 601 SVKSPKTVSP PIRKGWSMSE QSEESVGGRV AERKQVENAK ASKKNGNVGK 651 TTWQNKESKG ETGKRSKEGH SLEMENENLV ENGADSDEDD NSFLKQQSPQ 701 EPKSLNWSSF VDNTFAEEFT TQNQKSQDVE LWEGEVVKEL SVEEQIKRNR 751 YYDEDEDEE BLASTP hits Entry CG22818_1 from database TREMBL: "SREBP-2"; product: "mutant sterol regulatory element binding protem-2"; Cricetulus griseus SRD-2 mutant sterol regulatory element binding proteιn-2 (SREBP-2) mRNA, complete eds. Cricetulus griseus (Chinese hamster) Length = 839 Score = 1502 (528.7 bits), Expect = 3.9e-154, P = 3.9e-154 Identities = 290/380 (76%), Positives = 322/380 (84%) Entry S28507 from database PIR: transcription factor SF3 - common sunflower Length = 219 Score = 212 (74.6 bits), Expect = 6.3e-18, Sum P(2) = 6.3e-18 Identities = 36/82 (43%), Positives = 55/82 (67%) Entry NTLIMDOM_l from database TREMBL: "SF3"; product: "LIM-domain SF3 protein"; N.tabacum mRNA for LIM-domain protein Nicotiana tabacum (common tobacco) Length = 189 Score = 216 (76.0 bits), Expect = 1.0e-16, P = 1.0e-16 Identities = 42/94 (44%), Positives = 57/94 (60%) Alert BLASTP hits for DKFZphutel_18ιl9, frame 1 No Alert BLASTP hits found Pedant information for DKFZphutel_18ιl9, frame 1 Report for DKFZphutel_18ιl9.1 [LENGTH] 759 [MW] 85225.57 [pi] 6.41 [HOMOL] TREMBL :CG22818_1 gene: "SREBP-2"; product: "mutant sterol regulatory element binding prote n-2"; Cricetulus griseus SRD-2 mutant sterol regulatory element binding protein- 2 (SREBP-2) mRNA, complete eds. le-151 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YLR257w] 3e-05 [FUNCAT] 05.04 translation (initiation, elongation and termination) [S. cerevisiae, YGR162W TIF4631 - mRNA cap-binding protein] le-04 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YGR162w TIF4631 mRNA cap-bmding protein] le-04 [BLOCKS] BL00478B [PIRKW] zinc finger 9e-16 [PIRKW] DNA binding 9e-16 [SUPFAM] LIM metal-binding repeat homology 9e-16 [PROSITE] MYRISTYL 6 [PROSITE] LIM_DOMAIN_l 1 [PROSITE] AMIDATION 2 [PROSITE] CAMP_PHOSPHO_SITE 4 [PROSITE] CK2_PHOSPHO_SITE 28 [PROSITE] TYR_PHOSPHO_SITE 2 [PROSITE] PKC_PHOSPHO_SITE 15 [PROSITE] ASN_GLYCOSYLATION 6 [PFAM] LIM domain containing proteins [KW] Irregular [KW] 3D [KW] LOW COMPLEXITY 5.53 % SEQ MESSPFNRRQWTSLΞLRVTAKELSLVNKNKSSAIVEIFSKYQKAAEETNMEKKRSNTENL SEG lctl- SEQ SQHFRKGTLTVLKKKWENPGLGAESHTDSLRNSSTEIRHRADHPPAEVTSHAASGAKADQ SEG lctl- SEQ EEQIHPRSRLRSPPEALVQGRYPHIKDGEDLKDHSTESKKMENCLGESRHEVEKSEISEN SEG lctl- SEQ TDASGKIEKYNVPLNRLKMMFEKGEPTQTKILRAQSRΞASGRKISENSYSLDDLEIGPGQ SEG lctl- SEQ LSSSTFDSEKNESRRNLELPRLSETSIKDRMAKYQAAVSKQSSSTNYTNELKASGGEIKI SEG lctl- SEQ HKMEQKENVPPGPEVCITHQEGEKISANENSLAVRSTPAEDDSRDSQVKSEVQQPVHPKP SEG x lctl- SEQ LSPDSRASSLSESSPPKAMKKFQAPARETCVECQKTVYPMERLLANQQVFHISCFRCSYC SEG xxxxxxxxxxxxxxxx lctl- ETTTTEEETTTCEEEETTEEEETTTTBTTTT SEQ NNKLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHRPHKDLWASKNENEEILERPAQ SEG lctl- TCBCBTTBEEEETTEEEETTTTTTTTTTCCTTTTTTTCTTT SEQ LANARETPHSPGVEDAPIAKVGVLAASMEAKASSQQEKEDKPAETKKLRIAWPPPTELGS SEG lctl- SEQ SGSALEEGIKMSKPKWPPEDEISKPEVPEDVDLDLKKLRRSSSLKERSRPFTVAASFQST SEG xxxxxxxxxxxxxxxxxx lctl- SEQ SVKSPKTVSPPIRKGWSMSEQSEESVGGRVAERKQVENAKASKKNGNVGKTTWQNKESKG SEG lctl- SEQ ETGKRSKEGHSLEMENENLVENGADSDEDDNSFLKQQSPQEPKSLNWSSFVDNTFAEEFT SEG lctl- SEQ TQNQKSQDVELWEGEVVKELSVEEQIKRNRYYDEDEDEE SEG xxxxxxx lctl- Prosite for DKFZphutel_18ιl9.1 PS00001 29->33 ASN_GLYCOΞYLATION PDOC00001 PS00001 59->63 ASN_GLYCOSYLATION PDOC00001 PΞ00001 92->96 ASN_GLYCOSYLATION PDOC00001 PS00001 251->255 ASN_GLYCOSYLATION PDOC00001 PS00001 286->290 ASN_GLYCOSYLATION PDOC00001 PS00001 706->710 ASN_GLYCOSYLATION PDOC00001 PS00004 52->56 CAMP_PHOSPHO_SITE PDOC00004 PS00004 65->69 CAMP_PHOSPHO_SITE PDOC00004 PS00004 222->226 CAMP_PHOSPHO_SITE PDOC00004 PS00004 579->583 CAMP_PHOSPHO_SITE PDOC00004 PS00005 15->18 PKC_PHOSPHO_SITE PDOC00005 PS00005 19->22 PKC_PHOSPHO_SITE PDOC00005 PS00005 89->92 PKC_PHOSPHO_SITE PDOC00005 PS00005 158->161 PKC_PHOSPHO_SITE PDOC00005 PS00005 184->187 PKC_PHOSPHO_SITE PDOC00005 PS00005 220->223 PKC_PHOSPHO_SITE PDOC00005 PS00005 248->251 PKC_PHOSPHO_SITE PDOC00005 PS00005 253->256 PKC_PHOSPHO_SITE PDOC00005 PS00005 266->269 PKC_PHOSPHO_SITE PDOC00005 PS00005 525->528 PKC_PHOSPHO_SITE PDOC00005 PS00005 583->586 PKC_PHOSPHO_SITE PDOC00005 PS00005 601->604 PKC_PHOSPHO_SITE PDOC00005 PS00005 604->607 PKC_PHOSPHO_SITE PDOC00005 PS00005 642->645 PKC_PHOSPHO_ΞITE PDOC00005 PS00005 662->665 PKC_PHOSPHO_SITE PDOC00005 PS00006 19->23 CK2_PHOSPHO_SITE PDOC00006 PS00006 48->52 CK2_PHOSPHO_SITE PDOC00006 PS00006 55->59 CK2_PHOSPHO_SITE PDOC00006 PS00006 85->89 CK2_PHOSPHO_SITE PDOC00006 PS00006 93->97 CK2_PHOSPHO_SITE PDOC00006 PS00006 132->136 CK2_PHOSPHO_SITE PDOC00006 PS00006 168-M72 CK2_PHOSPHO_SITE PDOC00006 PS00006 230->234 CK2_PHOSPHO_ΞITE PDOC00006 PS00006 244->248 CK2_PHOSPHO_SITE PDOC00006 PS00006 2 66->270 CK2_PHOΞPHO_SITE PDOC00006 PS00006 294->298 CK2_PHOSPHO_SITE PDOC00006 PS00006 318->322 CK2_PHOSPHO_SITE PDOC00006 PS00006 326->330 CK2_PHOSPHO_SITE PDOC00006 PS00006 337->341 CK2 PHOSPHO SITE PDOC00006 PS00006 369-->373 CK2 PHOSPHO SITE PDOC00006 PS00006 389- ->393 CK2 PHOSPHO " " SITE PDOC00006 PS00006 467- ->471 CK2 PHOSPHO " " SITE PDOC00006 PS00006 514- ->518 CK2 PHOSPHO " " SITE PDOC00006 PS00006 543- ->547 CK2 PHOSPHO " " SITE PDOC00006 PS00006 563- ->567 CK2 PHOSPHO " " SITE PDOC00006 PS00006 583- ->587 CK2 PHOSPHO " " SITE PDOC00006 PS00006 617- ->621 CK2 PHOSPHO " " SITE PDOC00006 PS00006 658- ->662 CK2 PHOSPHO " " SITE PDOC00006 PS00006 686- ->690 CK2 PHOSPHO " " SITE PDOC00006 PS00006 698- ->702 CK2 PHOSPHO " " SITE PDOC00006 PS00006 709- ->713 CK2 PHOSPHO " " SITE PDOC00006 PS00006 714- ->718 CK2 PHOSPHO " " SITE PDOC00006 PS00006 741- ->745 CK2 PHOSPHO " " SITE PDOC00006 PΞ00007 223- ->230 TYR PHOSPHO " " SITE PDOC00007 PS00007 222- ->230 TYR PHOSPHO " " SITE PDOC00007 PS00008 2 3 9- ->245 MYRISTYL PDOC00008 PS00008 427- ->433 MYRISTYL PDOC00008 PS00008 502- ->508 MYRISTYL PDOC00008 PS00008 539- ->545 MYRISTYL PDOC00008 PS00008 548- ->554 MYRISTYL PDOC00008 PS00008 627- ->633 MYRISTYL PDOC00008 PS00009 220- ->224 AMIDATION PDOC00009 PS00009 662- ->666 AMIDATION PDOC00009 PS00478 390- ->425 LIM DOMAIN ] PDOC00382 Pfam for DKFZphutel_18ιl9.1 HMM_NAME LIM domain containing proteins HMM *CagCNrpIyDREιvMRAMNKvWHpECFrCcdCqqPLtegdeFYErDGrI C C++++Y+ E++ A+ V+H++CFRC+ C+ L+ G+ + ++ GRI Query 390 CVECQKTVYPMERLL-ANQQVFHISCFRCSYCNNKLSLGT-YASLHGRI 436 HMM YCKhDYYrrFg* YCK+++ ++F+ Query 437 YCKPHFNQLFK 447 DKFZphutel_18ι4 group: uterus derived DKFZphutel_18ι4 encodes a novel 220 amino acid protein without similarity to known proteins. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of uterus-specific genes . weak similarity to C. elegans D2085.2 complete cDNA, complete eds, few EST hits Sequenced by AGOWA Locus: /map="7q31" Insert length: 1568 bp Poly A stretch at pos. 1551, polyadenylation signal at pos. 1523 1 GCCGAGCGGA GAGGGTAGAG ACGGGGTTTC ACCGTGTTAG CCAAGATGGT 51 CTCGATCTCC TGACCTCGTG ATCCGCCCGC CTCGGCCTCC CAAAGTGCTG 101 GGATTACAGG CGTGAGCCAC TGCGCCCGGC CTGTTGTACA GTTATTAAAG 151 TTATCATTTA ACATGGAAGA AGATGAGTTC ATTGGAGAAA AAACATTCCA 201 ACGTTATTGT GCAGAATTCA TTAAACATTC ACAACAGATA GGTGATAGTT 251 GGGAATGGAG ACCATCAAAG GACTGTTCTG ATGGCTACAT GTGCAAAATA 301 CACTTTCAAA TTAAGAATGG GTCTGTGATG TCACATCTAG GAGCATCTAC 351 CCATGGACAG ACATGTCTTC CCATGGAGGA GGCTTTCGAG CTACCCTTGG 401 ATGATTGTGA AGTGATTGAA ACTGCAGCAG CGTCCGAAGT GATTAAATAT 451 GAGTATCATG TCTTATATTC CTGTAGCTAC CAAGTGCCTG TACTTTACTT 501 TAGGGCAAGC TTTTTAGATG GGAGACCTTT AACTCTGAAG GACATATGGG 551 AAGGAGTTCA TGAGTGCTAT AAGATGCGAC TGCTACAGGG ACCATGGGAC 601 ACTATTACGC AACAGGAACA TCCAATACTT GGGCAACCCT TTTTTGTACT 651 TCATCCCTGC AAGACGAATG AATTCATGAC TCCTGTATTA AAGAATTCTC 701 AGAAAATCAA TAAGAATGTC AACTATATCA CATCATGGCT GAGCATTGTA 751 GGGCCAGTTG TTGGGCTGAA TCTACCTCTG AGTTATGCCA AAGCAACGTC 801 TCAGGATGAA CGAAATGTCC CTTAACAAGA TTCTTCTATT GAGTTTAGGA 851 ATTGCGGCAC GAAGAATGCC AAGAGTTTAC CTGGCCAGCC CTGGCTTTAA 901 TAGGACTGAT ACCATGGAAT ATTTCATCTC ACCAAGATGT GACATGGATT 951 ATTTTTCCCT TGGACACAAA TGTCTACAGC AACTGATGTT TGATAGGCTG 1001 AATGTTTAGA AGAAACACTT CAAAGGGATA CATCATGGCC AGGCATGGTG 1051 GCTCACACCT GTAATCCAAG CACTTTGGGA GGCCAAGGTG GGAGCATCAC 1101 TTGATCCTGG GAGTTCGAGA CCAGCCTGGG CAACATGGTG AAACCCTGTC 1151 GGTACAAAAA AATACAAAAA TTTGCCTGTT TATGGTGGTG TGTTCCTGTA 1201 GTCCCAGCTC CCCAGGAGGC TGAGGTGGGA GGTTGGCTTT AACCCAGGAG 1251 GCAGAGGTTG CAGTGAGCTG AGACTGTGCC ACTGCAGTCC AGCCTGGGTG 1301 ACAGAGCCAG ACACTGTCTC GGGAAAAAAA AAAAAAAAAA AAAGACACAT 1351 CACTATAAAT AGCAAAAAAA CAAATCTAAC TTATTAA AC TAGGAATACC 1401 AACATTATTA GGGCACTTGC AGGTTATTCT TTTCTAGGCC AAGTACTTCA 1451 CTTCCATTTG TCTGACATGG AGATTGAGGG AGAAATGTAT TTGTGTGTTC 1501 ATTTTAATGT AAGATATATA AAAATTAAAT TACTGGATTT ACCTGTCCCT 1551 GAAAAAAAAA AAAAAAAA BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 1 ORF from 163 bp to 822 bp; peptide length: 220 Category: similarity to unknown protein 1 MEEDEFIGEK TFQRYCAEFI KHSQQIGDSW EWRPSKDCSD GYMCKIHFQI 51 KNGSVMSHLG ASTHGQTCLP MEEAFELPLD DCEVIETAAA SEVIKYEYHV 101 LYSCSYQVPV LYFRASFLDG RPLTLKDIWE GVHECYKMRL LQGPWDTITQ 151 QEHPILGQPF FVLHPCKTNE FMTPVLKNSQ KINKNVNYIT SWLSIVGPVV 201 GLNLPLSYAK ATSQDERNVP BLASTP hits Entry CED2085_2 from database TREMBL: "D2085.2"; Caenorhabditis elegans cosmid D2085 Length = 173 Score = 167 (58.8 bits), Expect = 1. le-12, P = 1. le-12 Identities = 36/121 (29%), Positives = 64/121 (52%) Alert BLASTP hits for DKFZphutel_18ι4, frame 1 No Alert BLASTP hits found Pedant information for DKFZphutel_18ι4, frame 1 Report for DKFZphutel_18ι4.1 [LENGTH] 220 [MW] 25278.99 [pi] 5.34 [HOMOL] TREMBL :CED2085_2 gene: "D2085.2"; Caenorhabditis elegans cosmid D2085 2e-ll [BLOCKS] BL00221E [PROSITE] MYRISTYL 2 [PROSITE] CK2_PHOSPHO_SITE [PROSITE] PKC_PHOSPHO_SITE [PROSITE] ASN_GLYCOSYLATION [KW] Alpha_Beta SEQ MEEDEFIGEKTFQRYCAEFIKHSQQIGDSWEWRPSKDCSDGYMCKIHFQIKNGΞVMSHLG PRD cccccccchhhhhhhhhhhhhhhhcccccccccccccccceeeeeeeeeeeccceeeeec SEQ ASTHGQTCLPMEEAFELPLDDCEVIETAAAΞEVIKYEYHVLYSCSYQVPVLYFRASFLDG PRD cccccccchhhhhhhhccccceeehhhhhchhhhhhhheeeeccccceeeeeeecccccc SEQ RPLTLKDIWEGVHECYKMRLLQGPWDTITQQEHPILGQPFFVLHPCKTNEFMTPVLKNΞQ PRD cccccchhhhhhhhhhhhhhhhccccccccccccccccceeeeccccccccccccccccc SEQ KINKNVNYITSWLSIVGPVVGLNLPLSYAKATSQDERNVP PRD ccccccccccccceeeeccccccccceeeecccccccccc Prosite for DKFZphutel_18ι4.1 PS00001 52->56 ASN_GLYCOSYLATION PDOC00001 PS00005 124->127 PKC_PHOSPHO_SITE PDOC00005 PS00005 179->182 PKC_PHOSPHO_SITE PDOC00005 PS00006 116-M20 CK2_PHOSPHO_SITE PDOC00006 PS00006 124->128 CK2_PHOSPHO_SITE PDOC00006 PS00006 149->153 CK2_PHOSPHO_SITE PDOC00006 PS00006 212->216 CK2_PHOSPHO_SITE PDOC00006 PS00008 53->59 MYRISTYL PDOC00008 PS00008 131->137 MYRISTYL PDOC00008 (No Pfam data available for DKFZphutel_18ι4.1) DKFZphutel 1811 group: nucleic acid management DKFZphtes3_15jl8 encodes a novel 184 amino acid protein with similarity to S. cerevisiae putative ribosomal protein YHR148w. The novel protein is similar to several 40S ribosomal proteins and therefore seems to part of the corresponding ribosome subunit. The new protein can find application in modulation of ribosome assembly, structure and function. strong similarity to S. cerevisiae YHR148w complete cDNA, complete eds, EST hits, potential start at Bp 45 matchs kozak consensus ANNatgG gene disruption of YHR148w is lethal 1 Sequenced by AGOWA Locus : unknown Insert length: 1076 bp Poly A stretch at pos. 1035, polyadenylation signal at pos. 1006 1 GCGCGCTCTC AGCTTCGGGT CCTGCGGCTG CGGCTGCCGC CATCATGGTG 51 CGGAAGCTTA AGTTCCACGA GCAGAAGCTG CTGAAGCAGG TGGACTTCCT 101 GAACTGGGAG GTCACCGACC ACAACCTGCA CGAGCTGCGC GTGCTGCGGC 151 GTTACCGGCT GCAGCGGCGG GAGGACTACA CGCGCTACAA CCAGCTGAGC 201 CGTGCCGTGC GTGAGCTGGC GCGGCGCCTG CGCGACCTGC CCGAACGCGA 251 CCAGTTCCGC GTGCGCGCTT CGGCCGCGCT GCTGGACAAG CTGTATGCTC 301 TCGGCTTGGT GCCCACGCGC GGTTCGCTGG AGCTCTGCGA CTTCGTCACG 351 GCCTCGTCCT TCTGCCGCCG CCGCCTCCCC ACCGTGCTCC TCAAGCTGCG 401 CATGGCGCAG CACCTTCAGG CTGCCGTGGC CTTTGTGGAG CAAGGGCACG 451 TACGCGTGGG CCCTGACGTG GTTACCGACC CCGCCTTCCT TGTCACGCGC 501 AGCATGGAGG ACTTTGTCAC TTGGGTGGAC TCGTCCAAGA TCAAGCGGCA 551 CGTGCTAGAG TACAATGAGG AGCGCGATGA CTTCGATCTG GAAGCCTAGC 601 GGATCTCCCA CTTTGCATGG CTGTCTTTTA CAGATGGGAA AACTGAGGCC 651 TGATGCTGGA GATTCTATGA GGGTGCTCTC CTCAAGGGTA TCAGACGGTC 701 GTAGGTTCTT AAGAATTTGA TTCATCAGTG GCAGGCCATG CATAGAGCCA 751 CGGGAGGTGC GTCCTTGTTT TCCAGGAAAT GTTCTTAGAA CTTGGACTAC 801 TGATTATTAA TTGACTGTGC CTTGGGAAAC AGTGGGAAGT AACTTGGTGC 851 AGCACTGGGG TATTGTTGGA CTGGTTCAAT TCGTTTAACT CGAATTCTTG 901 CTCCTGGCCG TGGTTAAGCT GTGTACAGAT GATGGAGAGT TTGGCCTCAA 951 GTTTTTATAA ACTGAGCGAG ACTAGTGTTC AGGATCTCCT CCCTTGTTTA 1001 AATGTCAATA AATGCCCCAA CTGCTTTGTA AGCTCAAAAA AAAAAAAAAA 1051 AAAAAAAAAA AAAAAAAAAA AAAAAA BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 3 ORF from 45 bp to 596 bp; peptide length: 184 Category: strong similarity to known protein 1 MVRKLKFHEQ KLLKQVDFLN WEVTDHNLHE LRVLRRYRLQ RREDYTRYNQ 51 LSRAVRELAR RLRDLPERDQ FRVRASAALL DKLYALGLVP TRGSLELCDF 101 VTASSFCRRR LPTVLLKLRM AQHLQAAVAF VEQGHVRVGP DVVTDPAFLV 151 TRSMEDFVTW VDSSKIKRHV LEYNEERDDF DLEA BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphutel_1811, frame 3 No Alert BLASTP hits found Pedant information for DKFZphutel_1811, frame 3 Report for DKFZphutel_1811.3 [LENGTH] 184 [MW] 21850.21 [pi] 9.54 [HOMOL] PIR:S33911 probable ribosomal protein YHR148w - yeast (Saccharomyces cerevisiae) 4e-•47 [ FUNCAT] 05.01 ribosomal proteins [S. cerevisiae, YHR148w] 2e-48 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YPL081w] 5e-07 [FUNCAT] j mrna translation and ribosome biogenesis [M. jannaschn, MJ0190] 8e-05 [BLOCKS] BL00632 [PIRKW] cytosol le-07 [PIRKW] ribosome le-07 [PIRKW] protein biosynthesis le-07 [SUPFAM] rat ribosomal protein S9 le-07 [PROSITE] MYRISTYL 1 [PROSITE] CK2_PHOSPHO_SITE 2 [PROSITE] TYR_PHOSPHO_SITE 1 [PROSITE] PKC_PHOSPHO_SITE 1 [PFAM] Ribosomal protein S4 [KW] All_Alpha [KW] LOW COMPLEXITY 6.52 % SEQ MVRKLKFHEQKLLKQVDFLNWEVTDHNLHELRVLRRYRLQRREDYTRYNQLSRAVRELAR SEG xxxxxxxxxxxx PRD ccchhhhhhhhhhhhhhhhhcccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh SEQ RLRDLPERDQFRVRASAALLDKLYALGLVPTRGSLELCDFVTASSFCRRRLPTVLLKLRM SEG PRD hhhhhccccchhhhhhhhhhhhhhhhccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhh SEQ AQHLQAAVAFVEQGHVRVGPDVVTDPAFLVTRSMEDFVTWVDSSKIKRHVLEYNEERDDF SEG PRD hhhhhhhhhhhhhhhccccceeecccceeeeeccccceeeeeccchhhhhhhhhcccccc SEQ DLEA SEG PRD Prosite for DKFZphutel_1811.3 PS00005 163->166 PKC_PHOSPHO_ΞITE PDOC00005 PS00006 153->157 CK2_PHOSPHO_SITE PDOC00006 PS00006 159->163 CK2_PHOSPHO_SITE PDOC00006 PS00007 41->49 TYR_PHOSPHO_SITE PDOC00007 PS00008 87->93 MYRISTYL PDOC00008 Pfam for DKFZphutel_1811.3 HMM_NAME Ribosomal protein S4 HMM *MSR.YRGPRWKIIRRPGElPWLTnK tklmrkYC..lRPgQHgWR M+R ++ +++K+++++++L W ++++R Y R+++ ++ Query 1 MVRKLKFHEQKLLKQVDFLNWEVTDHNLHELRVLRRYRLQRREDYTRYN 49 HMM qRktLsKIRRmSQYrlRLQEKQKLRFMYGNItERQLRRYvRiaEdKRKID Q + +R +++ + L+E + +R +++++L++++ +++ L Query 50 QLSR—AVRELARRLRDLPERDQFRVRASAALLDKLYALGLVP-TRGSLE 96 HMM YsTGenLMQILEMRLDNIVFRMGMAPTIHHARQLINHRHIRVNdRIVNIP ++ + ++++RL++++ ++ MA ++A+ +++++H+RV++ +V++P Query 97 LCDFVTASSFCRRRLPTVLLKLRMAQHLQAAVAFVEQGHVRVGPDVVTDP 146 HMM SYiCRPNDiISIRDkqrMQsHIkWmeSPegrmRPNHLErNnkkYeGtlN ++++++ + +++++W++ S+ ++R+ + Y+ + Query 147 AFLVTRS M EDFVTWVDSSK IKRHVLEYNEERD 178 HMM rllEReWiplklNElLVVEY* +++ + Query 179 DFDLE 183 DKFZphutel_19fl9 group: transmembrane protein DKFZphutel_19f19 encodes a novel 204 ammo acid protein with similarity to murine p24 protein. Murine p24 is expressed only in brain where it is localized exclusively in neurons. It seems to be a neuron-specific membrane protein localised in intracellular organelles of highly differentiated neural cells and may play a role in the neural organelle transport system. As p24, the novel protein contains 2 transmembrane regions, but it contains not the sequence homologous to the microtubule-binding domain of microtubule-associated proteins present in p24. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application m studying the expression profile of uterus-specific genes and as a new marker for uterine cells. similarity to mouse P24 protein ; membrane regions : 2 Summary DKFZphutel_19f19 encodes a novel 204 amino acid protein, with similarity to mouse P24 protein. similarity to mouse P24 protein complete cDNA, complete eds, EST hits, 2 TM-domams Sequenced by AGOWA Locus: /map=14.8 cR from top of Chr20 linkage group Insert length: 2042 bp Poly A stretch at pos. 1958, polyadenylation signal at pos. 1940 1 GCAGGCAGAG AGATGAGGAA ACTGAGACCC AGAAAGGTGG AAGCACTTGT 51 CTAAGGTCAC GCCTCCAGGA AGCAGTGTGT CCACGACTCC AGTCCAAGTG 101 GTCAGGCTCC AGAGCCCACA GTCCCAGGGG TCCATGATGC CGAGCTGCAA 151 TCGTTCCTGC AGCTGCAGCC GCGGCCCCAG CGTGGAGGAT GGCAAGTGGT 201 ATGGGGTCCG CTCCTACCTG CACCTCTTCT ATGAGGACTG TGCAGGCACT 251 GCTCTCAGCG ACGACCCTGA GGGACCTCCG GTCCTGTGCC CCCGCCGGCC 301 CTGGCCCTCA CTGTGTTGGA AGATCAGCCT GTCCTCGGGG ACCCTGCTTC 351 TGCTGCTGGG TGTGGCGGCT CTGACCACTG GCTATGCAGT GCCCCCCAAG 401 CTGGAGGGCA TCGGTGAGGG TGAGTTCCTG GTGTTGGATC AGCGGGCAGC 451 CGACTACAAC CAGGCCCTGG GCACCTGTCG CCTGGCAGGC ACAGCGCTCT 501 GTGTGGCAGC TGGAGTTCTG CTCGCCATCT GCCTCTTCTG GGCCATGATA 551 GGCTGGCTGA GCCAGGACAC CAAGGCAGAG CCCTTGGACC CCGAAGCCGA 601 CAGCCACGTG GAGGTCTTCG GGGATGAGCC AGAGCAGCAG TTGTCACCCA 651 TTTTCCGCAA TGCCAGTGGC CAGTCATGGT TCTCGCCACC CGCCAGCCCC 701 TTTGGGCAAT CTTCTGTGCA GACTATCCAG CCCAAGAGGG ACTCCTGAGC 751 TGCCCACATG GCCTAAGATG TGGGTCCTGG ATCCTTCCCC CTTCTCACCA 801 TAACCCCCTC TCAGTGTTTC CCCAACTTCT CCCTTTAGAG CCCAACTCCA 851 GGTCAAATCT GGAGCTCAAA TCCCAGTGCT CCCTCCCCAG GAGTGGGGCC 901 CCAACTCTTC CAAGATACCA GCATTCCTCA AGTCCTCCCA AAACTTCCTA 951 CCCACACCCT CTTCCCAAGG CCCTCAGGGG CAGAAAACAT CTCCTTCAAC 1001 CCGTCCCCAC TCCTTCCTCT GCATGACCTT GGGCAAACCC TTGCCCTTTC 1051 AAGCCATCAG CTCCTGCCTC TCTGCCATGA GGGCTTTGGA TCAGATTCCT 1101 CTTCTCGCCA GGATGAGGAC ACGCACTGCC CTCCATAGAC ACAGATGAAG 1151 GGGTGGGGGT CATTCAGCTC GAATGGGTCC CAGATGCTCA CTTGGCCTTT 1201 CCCTGCAGGA TGAGTGAAGA CGTTTGCCTC TCACAGTGTG TCTTCTACCT 1251 GCATTTTGGC ATCAGAGCCC CCCAGCCCAC CCACCACAGG CAATTACTAG 1301 CCCTAGTTGA TAGGTGAGGT GGGTGAAGAA GGCTGGAGGT GACATGTCCG 1351 AGGTCACACA ACAAAGCAGC ATGCAGGAAC TAGAAACACA TCTTCAGCCT 1401 CCTCCTGGGC CAGCTCTTGT GCTACAGGTG GGGCGGAGCC AGCCCCTCAC 1451 CTTCCTGGTT CCCTGAGGGT CCTCAGGGTG GAGGACAGGT TTGGCCCAGA 1501 AAGACTAGCC AGAGGCCTGA TGGTCCCAGG TGGCTCTGGA TATACTTTGG 1551 ATATGGATTT AAATGGTCTC TAAGAGCCGG GGGTAGGGGG CAGGAAAAGT 1601 GGGTTGTCTT TGCCCCTCAA AGTCCACCTA CCTAGAAACC AAGCCCACGG 1651 TCTTGGCCGT GACCCTGATA ATAAATGGGC TCTCTCAGAG GCGCCAGCCC 1701 CTCCCTCCCC AGCCGGAGGC GTCATCTCTC TTCTGTACCA CTAGAGGGAG 1751 CTCTGATGCA GCTGGAGAGC AGCGCTCAAG GCTCTCGCCC CTCCCCTCCC 1801 TAACCCTTAC CTTCAGTCTC CACCAGCCTG AAGGGCCTCC TAGGGGATCC 1851 TCAGGCGGCC CCCACCAGGG CACACCCTAC TGTCCTTGTG CCTCACGCCC 1901 CCTCCTCATC CTGCACCCCT TCCATCCCAC CTTCCCTTTC AATAAACAGC 1951 TGGGATGGAA AAAAAAAAAA AGAAAAAAAA AAAAAAAAAA AAAAAAAAAA 2001 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AA BLAST Results Entry HS417348 from database EMBL: human STS WI-14697. Length = 290 Minus Strand HSPs: Score = 1254 (188.2 bits), Expect = 3.0e-50, P = 3.0e-50 Identities = 262/273 (95%) Medline entries 97334404: A newly identified membrane protein localized exclusively m intracellular organelles of neurons. Peptide information for frame 2 ORF from 134 bp to 745 bp; peptide length: 204 Category: similarity to known protein 1 MMPSCNRSCS CSRGPSVEDG KWYGVRSYLH LFYEDCAGTA LSDDPEGPPV 51 LCPRRPWPSL CWKISLSSGT LLLLLGVAAL TTGYAVPPKL EGIGEGEFLV 101 LDQRAADYNQ ALGTCRLAGT ALCVAAGVLL AICLFWAMIG WLSQDTKAEP 151 LDPEADSHVE VFGDEPEQQL SPIFRNASGQ SWFSPPASPF GQSSVQTIQP 201 KRDS BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphutel_19f19, frame 2 TREMBL:MMP2000_1 product: "P24 protein"; Mouse mRNA for P24 protein, complete eds., N = 1, Score = 295, P = 3.8e-26 >TREMBL:MMP2000_1 product: "P24 protein" Mouse mRNA for P24 protein, complete eds. Length = 196 HSPs: Score = 295 (44.3 bits), Expect = 3.8e-26, P = 3.8e-26 Identities = 58/139 (41%), Positives = 81/139 (58%) Query: 2 MPSCNRSCSCSRGPSVEDGKW YGVRSYLHLFYEDCAGTALSDDPEGPPVLCPRRPWP 58 M SC+ +C R + +G + YGVRSYLH FYEDC + + + P R W Sbjct: 1 MTSCSNTCGSRRAQADTEGGYQQRYGVRSYLHQFYEDCTASIWEYEDDFQIQRΞPNR-WS 59 Query: 59 SLCWKISLSSGTLLLLLGVAALTTGYAVPPKLEGIGEGEFLVLDQRAADYNQALGTCRLA 118 S+ WK+ L SGT+ ++LG+ L G+ VPPK+E GE +F+V+D A YN AL TC+LA Sbjct: 60 ΞVFWKVGLIΞGTVFVILGLTVLAVGFLVPPKIEAFGEADFMVVDTHAVKYNGALDTCKLA 119 Query: 119 GTALCVAAGVLLAICLFWAM 138 G L G +A CL ++ Sbjct: 120 GAVLFCIGGTSMAGCLLMSV 139 Pedant information for DKFZphutel_19f19, frame 2 Report for DKFZphutel_19f19.2 [LENGTH] 204 [MW] 21983.07 [pi] 4.69 [HOMOL] TREMBL :MMP2000_1 product: "P24 protein"; Mouse mRNA for P24 protein, complete cds. 7e-19 [PROSITE] MYRISTYL 4 [PROSITE] CAMP_PHOSPHO_SITE 1 [PROSITE] CK2_PHOSPHO_SITE 3 [PROSITE] PKC_PHOSPHO_SITE 1 [PROSITE] ASN_GLYCOSYLATION 2 [KW] TRANSMEMBRANE 2 [KW] LOW_COMPLEXITY 10.29 % SEQ MMPSCNRSCSCSRGPΞVEDGKWYGVRSYLHLFYEDCAGTALSDDPEGPPVLCPRRPWPSL SEG PRD cccccccccccccccccccccceeehhhhhccccccccccccccccccccccccccccce MEM MM SEQ CWKISLSSGTLLLLLGVAALTTGYAVPPKLEGIGEGEFLVLDQRAADYNQALGTCRLAGT SEG .... xxxxxxxxxxxxxxxxxxxxx PRD eeeeeccccceeecccceeeecccccccccccccccceeeecccccccchhhhhhhhchh MEM MMMMMMMMMMMMMMMMMMMMMMMMMMM MMMMMMM SEQ ALCVAAGVLLAICLFWAMIGWLSQDTKAEPLDPEADSHVEVFGDEPEQQLSPIFRNASGQ SEG PRD hhhhhhhhhhhhhhhhhhhhhhccccccccccccccceeeeccccccccccccccccccc MEM MMMMMMMMMMMMMMMMMMMMMM SEQ SWFSPPASPFGQSSVQTIQPKRDS SEG PRD ccccccccccccceeeeccccccc MEM Prosite for DKFZphutel_19f19.2 PS00001 6->10 ASN_GLYCOSYLATION PDOC00001 PS00001 176->180 ASN_GLYCOSYLATION PDOC00001 PS00004 201->205 CAMP_PHOSPHO_SITE PDOC00004 PS00005 114->117 PKC_PHOSPHO_SITE PDOC00005 PS00006 16->20 CK2_PHOSPHO_SITE PDOC00006 PS00006 146->150 CK2_PHOSPHO_SITE PDOC00006 PS00006 157->161 CK2_PHOSPHO_SITE PDOC00006 PS00008 38->44 MYRISTYL PDOC00008 PΞ00008 92->98 MYRISTYL PDOC00008 PS00008 119->125 MYRISTYL PDOC00008 PS00008 127->133 MYRISTYL PDOC00008 (No Pfam data available for DKFZphutel_19f19.2 ) DKFZphutel_19gl9 group: uterus derived DKFZphutel_19gl9 encodes a novel 400 ammo acid protein, with strong but partial similarity to a bovine elastin-related protein expressed m fetal calf ligamentum nuchae. The novel protein contains 2 RGD cell attachment sites. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of uterus-specific genes and as a new marker for uterine cells. similarity to bovine elastin fragment complete cDNA, complete eds, EST hits Sequenced by AGOWA Locus: map=54.9 cR from top of Chr3 linkage group Insert length: 3244 bp Poly A stretch at pos. 3227, polyadenylation signal at pos. 3216 1 GTAACTGCAG TAAGTCCCGC TTGGCCCTGG AGTCCACGCG GATTTTCGAA 51 GCTGGGGCTG GCAAGAGGCC GCTGGACACC ACGCTCCAGT CGTCAGCCCA 101 CTTCCTAGCT GAACAGCGCG AGGCGGCGGC AGCGAGCCGG GTCCCACCAT 151 GGCCGCGAAT TATTCCAGTA CCAGTACCCG GAGAGAACAT GTCAAAGTTA 201 AAACCAGCTC CCAGCCAGGC TTCCTGGAAC GGCTGAGCGA GACCTCGGGT 251 GGGATGTTTG TGGGGCTCAT GGCCTTCCTG CTCTCCTTCT ACCTAATTTT 301 CACCAATGAG GGCCGCGCAT TGAAGACGGC AACCTCATTG GCTGAGGGGC 351 TCTCGCTTGT GGTGTCTCCT GACAGCATCC ACAGTGTGGC TCCGGAGAAT 401 GAAGGAAGGC TGGTGCACAT CATTGGCGCC TTACGGACAT CCAAGCTTTT 451 GTCTGATCCA AACTATGGGG TCCATCTTCC GGCTGTGAAA CTGCGGAGGC 501 ACGTGGAGAT GTACCAATGG GTAGAAACTG AGGAGTCCAG GGAGTACACC 551 GAGGATGGGC AGGTGAAGAA GGAGACGAGG TATTCCTACA ACACTGAATG 601 GAGGTCAGAA ATCATCAACA GCAAAAACTT CGACCGAGAG ATTGGCCACA 651 ATAACCCCAG TGCCATGGCA GTGGAGTCAT TCACGGCAAC AGCCCCCTTT 701 GTCCAAATTG GCAGGTTTTT CCTCTCGTCA GGCCTCATCG ACAAAGTCGA 751 CAACTTCAAG TCCCTGAGCC TATCCAAGCT GGAGGACCCT CATGTGGACA 801 TCATTCGCCG TGGAGACTTT TTCTACCACA GCGAAAATCC CAAGTATCCA 851 GAGGTGGGAG ACTTGCGTGT CTCCTTTTCC TATGCTGGAC TGAGCGGCGA 901 TGACCCTGAC CTGGGCCCAG CTCACGTGGT CACTGTGATT GCCCGGCAGC 951 GGGGTGACCA GCTAGTCCCA TTCTCCACCA AGTCTGGGGA TACCTTACTG 1001 CTCCTGCACC ACGGGGACTT CTCAGCAGAG GAGGTGTTTC ATAGAGAACT 1051 AAGGAGCAAC TCCATGAAGA CCTGGGGCCT GCGGGCAGCT GGCTGGATGG 1101 CCATGTTCAT GGGCCTCAAC CTTATGACAC GGATCCTCTA CACCTTGGTG 1151 GACTGGTTTC CTGTTTTCCG AGACCTGGTC AACATTGGCC TGAAAGCCTT 1201 TGCCTTCTGT GTGGCCACCT CGCTGACCCT GCTGACCGTG GCGGCTGGCT 1251 GGCTCTTCTA CCGACCCCTG TGGGCCCTCC TCATTGCCGG CCTGGCCCTT 1301 GTGCCCATCC TTGTTGCTCG GACACGGGTG CCAGCCAAAA AGTTGGAGTG 1351 AAAAGACCCT GGCACCCGCC CGACACCTGC GTGAGCCCTA GGATCCAGGT 1401 CCTCTCTCAC CTCTGACCCA GCTCCATGCC AGAGCAGGAG CCCCGGTCAA 1451 TTTTGGACTC TGCACCCCCT CTCCTCTTCA GGGGCCAGAC TTGGCAGCAT 1501 GTGCACCAGG TTGGTGTTCA CCAGCTCATG TCTTCCCCAC ATCTCTTCTT 1551 GCCAGTAAGC AGCTTTGGTG GGCAGCAGCA GCCATGAATG GCAAGCTGAC 1601 AGCTTCTCCT GCTGTTTCCT TCCTCTCTTG GACTGAGTGG GTACGGCCAG 1651 CCACTCAGCC CATTGGCAGC TGACAACGCA GACACGCTCT ACGGAGGCCT 1701 GCTGATAAAG GGCTCAGCCT TGCCGTGTGC TGCTTCTCAT CACTGCACAC 1751 AAGTGCCATG CTTTGCCACC ACCACCAAGC ACATCTGTGA TCCTGAAGGG 1801 CGGCCGTTAG TCATTACTGC TGAGTCCTGG GTCACCAGCA GACACACTGG 1851 GCATGGACCC CTCAAAGCAG GCACACCCAA AACACAAGTC TGTGGCTAGA 1901 ACCTGATGTG GTGTTTAAAA GAGAAGAAAC ACTGAAGATG TCCTGAGGAG 1951 AAAAGCTGGA CATATACTGG GCTTCACACT TATCTTATGG CTTGGCAGAA 2001 TCTTTGTAGT GTGTGGGATC TCTGAAGGCC CTATTTAAGT TTTTCTTCGT 2051 TACTTTGCTG CTTCATGTGT ACTTTCCTAC CCCAAGAGGA AGTTTTCTGA 2101 AATAAGATTT AAAAACAAAA CAAAAAAAAC ACTTAATATT TCAGACTGTT 2151 ACAGGAAACA CCCTTTAGTC TGTCAGTTGA ATTCAGAGCA CTGAAAGGTG 2201 TTAAATTGGG GTATGTGGTT TGATTGATAA AAAGTTACCT CTCAGTATTT 2251 TGTGTCACTG AGAAGCTTTA CAATGGATGC TTTTGAAACA AGTATCAGCA 2301 AAAGGATTTG TTTTCACTCT GGGAGGAGAG GGTGGAGAAA GCACTTGCTT 2351 TCATCCTCTG GCATCGGAAA CTCCCCTATG CACTTGAAGA TGGTTTAAAA 2401 GATTAAAGAA ACGATTAAGA GAAAAGGTTG GAAGCTTTAT ACTAAATGGG 2451 CTCCTTCATG GTGACGCCCC GTCAACCACA ATCAAGAACT GAGGCCTGAG 2501 GCTGGTTGTA CAATGCCCAC GCCTGCCTGG CTGCTTTCAC CTGGGAGTGC 2551 TTTCGATGTG GGCACCTGGG CTTCCTAGGG CTGCTTCTGA GTGGTTCTTT 2601 CACGTGTTGT GTCCATAGCT TTAGTCTTCC TAAATAAGAT CCACCCACAC 2651 CTAAGTCACA GAATTTCTAA GTTCCCCAAC TACTCTCACA CCCTTTTAAA 2701 GATAAAGTAT GTTGTAACCA GGATGTCTTA AATGATTCTT TGTGTACCTT 2751 TTCTGTCATA TTCAGAAACC GTTTTGTGCC TGCTGGGAGT AATTCCTTTA 2801 GCAATTAAGT ATTTGGTAGC TGAATAAGGG GTCAGAACTT CTGAAACCAG 2851 AGATCTGTAA TCATCTCTAT TGGCCTGGGG TGCCTGTGCT ATAAATGAGT 2901 TTCTTCACAT GAAAAACACA GCCAGCCCAA GATGACTTAT CTGGGTTTAG 2951 GATTCAATAG TATTCACTAA CTGCTTATTA CATGAGCAAT TTCATCAAAT 3001 CTCCAAACTC TTAAAGGATG CTTTCGGAAA ACACGCTGTA TACCTAGATG 3051 ATGACTAAAT GCAAAATCCT TGGGCTTTGG TTTTTTTCTA GTAAGGATTT 3101 TAAATAACTG CCGACTTCAA AAGTGTTCTT AAAACGAAAG ATAATGTTAA 3151 GAAAAATTTG AAAGCTTTGG AAAACCAAAT TTGTAATATC ATTGTATTTT 3201 TTATTAAAAG TTTTGTAATA AATTTCTAAA AAAAAAAAAA AAAA BLAST Results Entry HS545355 from database EMBL: human STS WI-14815. Length = 436 Minus Strand HSPs: Score = 2040 (306.1 bits), Expect = 6.2e-86, P = 6.2e-86 Identities = 420/426 (98%) Entry HS932147 from database EMBL: human STS WI-8531. Length = 341 Minus Strand HSPs: Score = 1705 (255.8 bits), Expect = 4.7e-70, P = 4.7e-70 Identities = 341/341 (100%) Medline entries 86051793: Bovine elastm cDNA clones: evidence for the occurrence of a new elastin-related protein in fetal calf ligamentum nuchae. Peptide information for frame 2 ORF from 149 bp to 1348 bp; peptide length: 400 Category: similarity to known protein 1 MAANYSSTST RREHVKVKTS SQPGFLERLS ETSGGMFVGL MAFLLSFYLI 51 FTNEGRALKT ATSLAEGLSL VVΞPDSIHSV APENEGRLVH IIGALRTSKL 101 LSDPNYGVHL PAVKLRRHVE MYQWVETEES REYTEDGQVK KETRYSYNTE 151 WRSEIINSKN FDREIGHNNP SAMAVESFTA TAPFVQIGRF FLSSGLIDKV 201 DNFKSLSLSK LEDPHVDIIR RGDFFYHSEN PKYPEVGDLR VSFSYAGLSG 251 DDPDLGPAHV VTVIARQRGD QLVPFSTKSG DTLLLLHHGD FSAEEVFHRE 301 LRSNSMKTWG LRAAGWMAMF MGLNLMTRIL YTLVDWFPVF RDLVNIGLKA 351 FAFCVATSLT LLTVAAGWLF YRPLWALLIA GLALVPILVA RTRVPAKKLE BLASTP hits Entry 145887 from database PIR: elastm - bovine (fragment) Length = 40 Score = 131 (46.1 bits), Expect = 4.9e-08, P = 4.9e-08 Identities = 31/41 (75%), Positives = 34/41 (82%) Alert BLASTP hits for DKFZphutel_19gl9, frame 2 No Alert BLASTP hits found Pedant information for DKFZphutel_19gl9, frame 2 Report for DKFZphutel_19gl9.2 [LENGTH] 400 [MW] 44831.53 [pi ] 7.23 [HOMOL] PIR:I45887 elastin bovine (fragment) le-06 [PROSITE] RGD 2 [PROSITE] MYRISTYL 3 [PROSITE] CAMP_PHOSPHO_SITE [PROSITE] CK2_PHOSPHO_SITE [PROSITE] TYR_PHOSPHO_SITE [PROSITE] PKC_PHOSPHO_SITE [PROSITE] ASN_GLYCOSYLATION [KW] TRANSMEMBRANE 4 SEQ MAANYSSTSTRREHVKVKTSSQPGFLERLSETSGGMFVGLMAFLLSFYLIFTNEGRALKT PRD ccceeecccceeeeeeeecccccceeeecccccccchhhhhhhhhhheeeeecccchhhh MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM.. SEQ ATSLAEGLSLVVSPDSIHSVAPENEGRLVHIIGALRTSKLLSDPNYGVHLPAVKLRRHVE PRD hhhhhccceeeeccccceeeeccccceeeeeeeeeeceeeccccccccccchhhhhhhhh MEM SEQ MYQWVETEEΞREYTEDGQVKKETRYSYNTEWRSEIINSKNFDREIGHNNPSAMAVESFTA PRD hheeehhhhheeecccccccceeeccccccceeeeeeccccceeecccccceeeeeeecc MEM M SEQ TAPFVQIGRFFLSSGLIDKVDNFKSLΞLSKLEDPHVDIIRRGDFFYHSENPKYPEVGDLR PRD ccceeeeeeeeeccccccccccceeeeeeeccccceeeeecccceeecccccccccccee MEM MMMMMMMMMMMMMMMMM SEQ VSFSYAGLSGDDPDLGPAHVVTVIARQRGDQLVPFSTKSGDTLLLLHHGDFSAEEVFHRE PRD eeccccccccccccccceeeeeeeeecccccccccccccceeeeeecccccchhhhhhhh MEM SEQ LRSNSMKTWGLRAAGWMAMFMGLNLMTRILYTLVDWFPVFRDLVNIGLKAFAFCVATSLT PRD hhccccccccchhhhhhhhhhhchhhhhhhhheeecccccccccccceeeeeeeeehhhh MEM MMMMMMMMMMMMMMMMMMMMMMMM MMMM SEQ LLTVAAGWLFYRPLWALLIAGLALVPILVARTRVPAKKLE PRD hhhhhccceeehhhhhhhhhhhhchhhhhhhhcccccccc MEM MMMMMMMMMMMMMMMMMMMMMMMMM Prosite for DKFZphutel_19gl9.2 PS00001 4->8 ASN_GLYCOSYLATION PDOC00001 PS00004 140-5-144 CAMP_PHOSPHO_SITE PDOC00004 PS00005 9->12 PKC_PHOSPHO_SITE PDOC00005 PS00005 10->13 PKC_PHOSPHO_SITE PDOC00005 PS00005 97->100 PKC_PHOSPHO_SITE PDOC00005 PS00005 276->279 PKC_PHOSPHO_SITE PDOC00005 PS00005 305->308 PKC_PHOSPHO_SITE PDOC00005 PS00006 10->14 CK2_PHOSPHO_SITE PDOC00006 PS00006 63->67 CK2_PHOSPHO_SITE PDOC00006 PS00006 209->213 CK2_PHOSPHO_SITE PDOC00006 PS00006 249->253 CK2_PHOSPHO_SITE PDOC00006 PS00006 292->296 CK2_PHOSPHO_SITE PDOC00006 PS00006 332->336 CK2_PHOSPHO_SITE PDOC00006 PS00007 220->227 TYR_PHOSPHO_SITE PDOC00007 PS00007 99->107 TYR_PHOSPHO_SITE PDOC00007 PS00008 35->41 MYRISTYL PDOC00008 PS00008 93->99 MYRISTYL PDOC00008 PS00008 310->316 MYRISTYL PDOC00008 PS00016 221->224 RGD PDOC00016 PS00016 268->271 RGD PDOC00016 (No Pfam data available for DKFZphutel_19gl9.2) DKFZphutel_19g22 group: cell structure and motility DKFZphutel_19g22 encodes a novel 390 ammo acid protein with very strong similarity to tuftelin/enamelin . Tuftelin/enamelin are matrix proteins of the teeth. As other proteins involved m calcification, these proteins are also expressed in the uterus matrix. The new protein can find application in modulation of tissue-calcification, especially the uterus . complete cDNA, complete eds start at Bp 51, EST hits in 3' UTR, human homolog of mouse tuftelin tuftelin is descriebed as a matrix protein of teeth but it seems also to be pressend in the uterus matrix Sequenced by AGOWA Locus : unknown Insert length: 3110 bp Poly A stretch at pos. 3093, polyadenylation signal at pos. 3071 1 GCAGACAGCG GGGTGGACAA GTGGCGTGTG TGCTGCGACC CCGAGGGAAG 51 ATGAACGGGA CGCGGAACTG GTGTACCCTG GTGGACGTGC ACCCAGAGGA 101 CCAGGCGGCG GGCAGCGTGG ACATTCTCAG GCTGACTCTC CAGGGTGAAC 151 TGACAGGAGA TGAACTTGAA CACATAGCCC AGAAGGCGGG CAGGAAGACC 201 TATGCCATGG TGTCCAGCCA CTCAGCTGGT CATTCTCTGG CTTCAGAACT 251 GGTGGAGTCC CATGATGGAC ATGAGGAGAT CATTAAGGTG TACTTGAAGG 301 GGAGGTCTGG AGACAAGATG ATTCACGAGA AGAATATTAA CCAGCTGAAG 351 AGTGAGGTCC AGTACATCCA GGAGGCCAGG AACTGCCTAC AGAAGCTCCG 401 GGAGGATATA AGTAGCAAGC TTGACAGGAA CCTAGGAGAT TCTCTCCATC 451 GACAGGAGAT ACAGGTGGTG CTAGAAAAGC CAAATGGCTT TAGTCAGAGT 501 CCCACAGCCC TGTACAGCAG CCCACCTGAG GTGGACACCT GTATAAATGA 551 GGATGTTGAG AGCTTGAGGA AGACGGTGCA GGACTTGCTG GCCAAGCTTC 601 AGGAGGCCAA GCGGCAACAC CAGTCAGACT GTGTGGCTTT TGAGGTCACA 651 CTCAGCCGGT ACCAGAGGGA AGCAGAACAA AGTAATGTGG CCCTTCAGAG 701 AGAGGAGGAC AGAGTGGAGC AGAAAGAGGC AGAAGTCGGA GAGCTGCAGA 751 GGCGCTTGCT AGGGATGGAG ACGGAGCATC AGGCCTTACT GGCGAAAGTG 801 AGGGAAGGGG AGGTGGCCCT AGAGGAACTT CGGAGCAACA ATGCTGACTG 851 CCAAGCAGAA CGAGAAAAGG CTGCTACCCT GGAAAAGGAA GTGGCCGGGT 901 TGCGGGAGAA GATCCACCAC TTGGATGACA TGCTCAAGAG CCAGCAGCGG 951 AAAGTCCGGC AAATGATAGA GCAGCTCCAG AATTCAAAAG CTGTGATCCA 1001 GTCAAAGGAC GCCACCATCC AGGAGCTCAA GGAGAAAATC GCCTATCTGG 1051 AGGCAGAGAA TTTAGAGATG CATGACCGGA TGGAACACCT GATAGAAAAA 1101 CAAATCAGTC ATGGCAACTT CAGCACCCAG GCCCGGGCCA AGACAGAGAA 1151 CCCGGGCAGT ATTAGGATAT CCAAGCCGCC TAGCCCGAAG CCCATGCCTG 1201 TCATCCGAGT GGTGGAAACC TGAGCTGCCT GGAGATGGTT GCTGCCATTG 1251 CTGCTGCCTC TGCCTCGGAG AAGCCCACTG CCCCTGTTGG CTGTTAACAC 1301 TGCCTTTGAC TTCCTGACTG TCCCCTGGCT GCACCCAGGA CTTCGGGCTC 1351 CTGTGTCTCA CCATTCCCAA GCCCCTGGCC ACTCTAAGCT GGGCAGACGG 1401 AGCACGAGCA CCTATTCAAG GCACTGCAGC CCTTTGGAAG ACATTGTCCT 1451 GCAAGCAGGA GCCAGGGCAA TATCTATATT CCTACAGTGA CTATTTTTCT 1501 CTGTAGAGAG CCTCCCTTCT GTTGTAGACT GGACTCTGGC TGCGCCATAA 1551 GCCAGGCCTT CATCAGATTG GGAGAGGTGA CAAGATTTGC CTCAGCCCTA 1601 AAAGCTGGAG ACACAGATGT CCAGAGTGAT TGGAGAATGT CCTGGGGGAA 1651 TGAAGTTCCT TCCACAAACA CAGCTCAGTT CTTAGCAACA AACTGTTTGT 1701 TTTTCTACTT GCTCCATCTG CAGCCTACGC TGCCCTGGCC TCCTGCAGAC 1751 AGATAGTGGG GTTACCTGGC AAGGCCTGGT GAGAGCCAGT GAACCTAAGC 1801 TTTGACTGGG TGGCCTTGTC TTTCTGGGGA GGAGGGAATG TACATTCAGG 1851 GAGTAGCCTT TTGCGGAAAA ATTCTCTAGG GCTACAGACA GTCATGTGTG 1901 ACTTCTCTCT GCTGTGAAAA CTCCCAGAGT CTCTTTAGGG ATTTTCCCTA 1951 AGGTGTACCA CCAGGCACAC CTCAGTCTTC TTGACCCAGA GCCTGAAAAC 2001 TGTTTTCACT GGGTTCCACC AGTCCCAGCA AAATCCTCTT TGTATTTATT 2051 TTGCTAAGTT ATTGGTGGTT TTGCTTACAT CTCATGATTG ATATAATACC 2101 AAAGTTCTAT AGCCTTCTCT TGCAGTATTT GGATTTGCTT GAAACCGGGA 2151 AAACTGTTCC CATTAGGCTT GTTAATGTCA GAGTGACACT ATTATGAATC 2201 TTTCTCTCCC TTTCCTCTGC CTGTTTCTTC TCTCTTTCTC CTTCAAACTT 2251 GCTCTGCAGC TAAGGAAGGT GAGTCTACTT TCCCTGAGGC TTTGGGGTCA 2301 GAGTATATGT TGTTTGGAGA AAGAGGGCAA TCAGGACTCT' TCTGGGACCC 2351 AGATGAGTTC TTCACTAGCC CTTCTGAACC CCTTGCTCCA TAATTGGTCT 2401 TTTATCCTGG CTCTGAATGA CCCTGCAGGT CATCATGGTT TTCTTTTTTT 2451 ATTGTTTTTT TTTTTTTCTG AGACAGAGTC TCACTCTGTC ACCCAGGCTG 2501 GAGTGCAGTG GCGCGATCTC AGCTCACTGC AACCTCTGCC TCCCGGATTT 2551 AAGCGATTCT TCTGCCTCAG CCTCCCGAGT AGCTGGGACT ACAGGTGTGC 2601 CACCACGCCT GGCTGATTTT TGTATTTTTA GTAGAGATGG GGTTTCACCA 2651 TACTGGCTAG GCTGGTCTCG AATTCCTGAC CTCAGGTGAT CCACCCACCT 2701 CGGCTTCCCA AAGTGCTAGG ATTATAGGCT TGAGCTACTG TGCCCGGCCC 2751 ATGGTGTTTT TCTTTAGGGC TCTTCCTACA GCCTTGAGAA GTAGATAGGC 2801 ATCAGAGTAT GGTACTATAG GAATCAGAAA AATTCAAAAC AAATGTGGAT 2851 TAAGTGTTTA GGCTCTATGT GGCTCACGCA GCCAGAATCC TTAAGTCTGT 2901 GTGTTTCTGT GTCTCAAGAC TGGGCTCACA TTCTGGCTTT GTCCATAACA 2951 ATGCTCTGGG ATTTCAGGGA GTTCCCTCAT TTGTAAAATG AGGGGGTCAG 3001 AGCAGGTGAT ATCCATGTTT CTTCCCTTTC TGATATTGTT GTCTGTGGCA 3051 TATTCTTTGT ATGGCGAATT TAATAAATTA TATTAATGTG TCTAAAAAAA 3101 AAAAAAAAAA BLAST Results No BLAST result Medline entries 98200312: Tuftelιn--aspects of protein and gene structure 97228909: Timing of the expression of enamel gene products during mouse tooth development . 91340750: Sequencing of bovine enamelm ("tuftelin") a novel acidic enamel protein. Peptide information for frame 3 ORF from 51 bp to 1220 bp; peptide length: 390 Category: strong similarity to known protein 1 MNGTRNWCTL VDVHPEDQAA GSVDILRLTL QGELTGDELE HIAQKAGRKT 51 YAMVSSHSAG HSLASELVES HDGHEEIIKV YLKGRSGDKM IHEKNINQLK 101 SEVQYIQEAR NCLQKLREDI SSKLDRNLGD SLHRQEIQVV LEKPNGFSQS 151 PTALYSSPPE VDTCINEDVE SLRKTVQDLL AKLQEAKRQH QSDCVAFEVT 201 LSRYQREAEQ SNVALQREED RVEQKEAEVG ELQRRLLGME TEHQALLAKV 251 REGEVALEEL RSNNADCQAE REKAATLEKE VAGLREKIHH LDDMLKSQQR 301 KVRQMIEQLQ NSKAVIQSKD ATIQELKEKI AYLEAENLEM HDRMEHLIEK 351 QISHGNFSTQ ARAKTENPGS IRISKPPSPK PMPVIRVVET BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphutel_19g22, frame 3 No Alert BLASTP hits found Pedant information for DKFZphutel_19g22, frame 3 Report for DKFZphutel_19g22.3 [LENGTH] 390 [MW] 44264.09 [pi] 5.68 [HOMOL] TREMBL:AF047704_1 product: "tuftelin"; Mus musculus tuftelin mRNA, complete cds. 0.0 [FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YDL058w] 2e-ll [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YDL058w] 2e-ll [ FUNCAT] 1 genome replication, transcription, recombination and repair [M. jannaschn, MJ1643] 7e-ll [FUNCAT] 09.13 biogenesis of chromosome structure [S. cerevisiae, YLR086w] le-08 [FUNCAT] 03.22.01 cell cycle check point proteins [S. cerevisiae, YGL086w] 6e-08 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YGL086w] 6e-08 [ FUNCAT] 03.13 meiosis [S. cerevisiae, YNL250w] 7e-08 [FUNCAT] 03.19 recombination and dna repair [S. cerevisiae, YNL250w] 7e-08 [FUNCAT] 11.04 dna repair (direct repair, base excision repair and nucleotide excision repair) [S. cerevisiae, YKR095w] le-07 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YDR285w] 2e-07 [FUNCAT] 30.13 organization of chromosome structure [S. cerevisiae, YDR285w] 2e-07 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YOR216c] le-05 [FUNCAT] 01.03.16 polynucleotide degradation [S. cerevisiae, YNL243w] le-04 [FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YNL243w] le-04 [FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YNL243w] le-04 [FUNCAT] 03.07 pheromone response, mat g-type determination, sex-specific proteins [S. cerevisiae, YNL243w] le-04 [FUNCAT] 08.19 cellular import [S. cerevisiae, YNL243w] le-04 [FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YNL243w] le-04 [FUNCAT] 08.22 cytoskeleton-dependent transport [S. cerevisiae, YHR023w MYOl - myosιn-1 isoform] 4e-04 [FUNCAT] 03.25 cytokinesis [S. cerevisiae, YHR023w MYOl - myosιn-1 isoform] 4e-04 [FUNCAT] 09.10 nuclear biogenesis [S. cerevisiae, YDR356w] 4e-04 [FUNCAT] 30.05 organization of centrosome [S. cerevisiae, YMR294w] 7e-04 [EC] 3.6.1.32 Myosin ATPase 8e-09 [PIRKW] blocked amino end le-07 [PIRKW] nucleus le-06 [PIRKW] citrulline le-07 [PIRKW] tandem repeat 8e-09 [PIRKW] heterodimer 3e-06 [PIRKW] DNA repair 2e-06 [PIRKW] heart 8e-09 [PIRKW] endocytosis 3e-07 [PIRKW] transmembrane protein 4e-10 [PIRKW] zinc fmger 3e-07 [PIRKW] metal binding 3e-07 [PIRKW] muscle contraction 8e-09 [PIRKW] acetylated amino end le-06 [PIRKW] actin binding 8e-09 [PIRKW] microtubule binding le-06 [PIRKW] cell division control le-06 [PIRKW] ATP 8e-09 [PIRKW] chromosomal protein 3e-06 [PIRKW] thick filament 8e-09 [PIRKW] phosphoprotem le-145 [PIRKW] skeletal muscle 8e-09 [PIRKW] calcium binding le-07 [PIRKW] meiosis 2e-06 [PIRKW] alternative splicing 7e-08 [PIRKW] DNA condensation 3e-06 [PIRKW] coiled coil 4e-10 [PIRKW] P-loop 8e-09 [PIRKW] heptad repeat le-07 [PIRKW] methylated ammo acid 8e-09 [PIRKW] lmmunoglobulm receptor 2e-06 [PIRKW] peripheral membrane protein 3e-07 [PIRKW] cardiac muscle 8e-09 [PIRKW] hydrolase 8e-09 [PIRKW] muscle 7e-08 [PIRKW] EF hand le-07 [PIRKW] cytoskeleton 7e-08 [PIRKW] hair le-07 [PIRKW] smooth muscle 7e-08 [PIRKW] calmodulin binding 3e-07 [SUPFAM] conserved hypothetical P115 protein 2e-09 [SUPFAM] myosin heavy chain 8e-09 [SUPFAM] RAD50 protein 2e-06 [SUPFAM] calmodulin repeat homology le-07 [SUPFAM] myosin motor domain homology 8e-09 [SUPFAM] alpha-actmin actin-binding domain homology le-06 [SUPFAM] tropomyosm 7e-08 [SUPFAM] protein-tyrosine kinase ret 3e-07 [SUPFAM] plectm le-06 [SUPFAM] tπchohyalin le-07 [SUPFAM] pleckstrin repeat homology 2e-06 [SUPFAM] ribosomal protein S10 homology le-06 [SUPFAM] protein kinase homology 3e-07 [SUPFAM] protein kinase C zinc-binding repeat homology 2e-06 [SUPFAM] giantin 4e-06 [SUPFAM] kinesin-related protein KLPA le-06 [SUPFAM] kinesin motor domain homology le-06 [SUPFAM] human early endosome antigen 1 3e-07 [SUPFAM] M5 protein 2e-06 [PROSITE] MYRISTYL 1 [PROSITE] AMIDATION 1 [PROSITE] CK2 PHOSPHO_SITE 6 [PROSITE] PKC PHOSPHO SITE 4 [PROSITE] ASN GLYCOSYLATION 2 [KW] All Alpha [KW] LOW COMPLEXITY 4.62 [KW] COILED COIL 35.13 SEQ MNGTRNWCTLVDVHPEDQAAGSVDILRLTLQGELTGDELEHIAQKAGRKTYAMVSSHSAG SEG PRD cccccceeeeeeeccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS SEQ HSLASELVESHDGHEEIIKVYLKGRΞGDKMIHEKNINQLKSEVQYIQEARNCLQKLREDI SEG PRD hhhhhhhhhhhhhhhhhhhhhhhccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS SEQ SSKLDRNLGDSLHRQEIQVVLEKPNGFSQSPTALYSSPPEVDTCINEDVESLRKTVQDLL SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCC SEQ AKLQEAKRQHQSDCVAFEVTLSRYQREAEQSNVALQREEDRVEQKEAEVGELQRRLLGME SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCC CCCCCCCCCCCCCCCCCCCCCCCCCC SEQ TEHQALLAKVREGEVALEELRSNNADCQAEREKAATLEKEVAGLREKIHHLDDMLKΞQQR SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CC CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC SEQ KVRQMIEQLQNSKAVIQSKDATIQELKEKIAYLEAENLEMHDRMEHLIEKQISHGNFSTQ SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCC CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC SEQ ARAKTENPGSIRISKPPSPKPMPVIRVVET SEG xxxxxxxxxxxxxxxxx ... PRD hhcccccccceeeecccccccccceeeccc COILS Prosite for DKFZphutel_19g22.3 PS00001 2->6 ASN_GLYCOSYLATION PDOC00001 PS00001 356->360 ASN_GLYCOSYLATION PDOC00001 PS00005 121->124 PKC_PHOSPHO_SITE PDOC00005 PS00005 171->174 PKC_PHOSPHO_SITE PDOC00005 PS00005 370->373 PKC_PHOSPHO_SITE PDOC00005 PS00005 378->381 PKC_PHOSPHO_SITE PDOC00005 PS00006 9->13 CK2_PHOSPHO_SITE PDOC00006 PS00006 35->39 CK2_PHOSPHO_SITE PDOC00006 PS00006 122->126 CK2_PHOSPHO_ΞITE PDOC00006 PS00006 157->161 CK2_PHOSPHO_SITE PDOC00006 PS00006 175->179 CK2_PHOSPHO_SITE PDOC00006 PS00006 322->326 CK2_PHOSPHO_SITE PDOC00006 PS00008 355->361 MYRISTYL PDOC00008 PS00009 46->50 AMIDATION PDOC00009 (No Pfam data available for DKFZphutel_19g22.3) DKFZphutel_19hl7 group: intracellular transport and trafficking DKFZphutel_19hl7 encodes a novel 879 amino acid protein, with similarity to N.crassa osbP oxysterol-bmding protein. The novel protein contains a oxysterol-binding protein family signature. Mammalian oxysterol- binding protein (OSBP) is a protein binds a variety of oxysterols (oxygenated derivatives of cholesterol) . OSBP seems to play a complex role in the regulation of sterol metabolism. OSBP is a cytosolic/Golgi receptor for oxysterols such as 25-hydroxycholesterol, and thus a potential target of siphingomyelm turnover and cholesterol mobilization at the plasma membrane and/or Golgi apparatus. Therefore, the new protein seems to be involved in oxysterol metabolism. The new protein can find application in modulating the response of cells to oxysterols. The protein can be used as marker for the golgi system. The Protein might be used to direct drugs to the golgi system m response to oxidative stess. strong similarity to C. elegans ZK1086.1 and oxysterol-binding proteins complete cDNA, complete eds, few EST hits similarity to proteins involved m steroid biosynthesis Sequenced by AGOWA Locus: unknown Insert length: 3828 bp Poly A stretch at pos. 3811, polyadenylation signal at pos. 3784 1 GCCCGCGCGC CCGGCCGGCC CGGAGCACCG AGCTCGCGGC ACGGTAGGAG 51 AAGCCCCCGA GCGCCCACAG CATGAAGGAG GAGGCCTTCC TCCGGCGCCG 101 CTTCTCCCTG TGTCCACCTT CCTCCACCCC TCAGAAAGTC GACCCCCGGA 151 AGCTCACCCG GAACTTGCTC CTCAGCGGAG ACAATGAGCT CTACCCACTC 201 AGCCCAGGGA AGGACATGGA GCCCAACGGC CCGTCGCTGC CCAGGGATGA 251 AGGGCCCCCG ACCCCAAGCT CTGCCACGAA GGTGCCACCG GCAGAGTACA 301 GGCTGTGCAA CGGGTCAGAC AAGGAATGTG TGTCCCCCAC CGCCAGGGTC 351 ACCAAGAAGG AGACTCTCAA GGCGCAGAAG GAGAACTACC GGCAGGAGAA 401 GAAGCGCGCC ACACGGCAGC TGCTCAGCGC TCTGACAGAC CCCAGCGTGG 451 TCATCATGGC TGACAGCCTG AAGATCCGCG GCACCCTGAA GAGCTGGACC 501 AAGCTGTGGT GCGTGCTGAA GCCGGGGGTG CTGCTCATCT ACAAGACGCC 551 CAAGGTGGGC CAGTGGGTGG GCACGGTGCT GCTGCACTGC TGCGAGCTCA 601 TCGAGCGGCC CTCCAAGAAG GACGGCTTCT GCTTCAAGCT CTTCCACCCG 651 CTGGATCAGT CCGTCTGGGC CGTGAAGGGC CCCAAAGGTG AGAGCGTGGG 701 CTCCATCACA CAGCCCCTGC CCAGCAGCTA CCTGATCTTC AGGGCCGCCT 751 CCGAGTCAGA TGGTCGCTGC TGGCTGGACG CCCTGGAGCT GGCCCTGCGC 801 TGCTCTAGCC TACTGAGACT GGGCACCTGC AAGCCGGGCC GAGACGGGGA 851 GCCAGGGACC TCGCCAGACG CATCACCCTC ATCGCTCTGT GGGCTGCCAG 901 CCTCAGCCAC TGTCCACCCA GACCAAGACC TGTTCCCACT GAACGGGTCT 951 TCCCTGGAGA ACGATGCATT CTCAGACAAG TCGGAGAGAG AGAACCCTGA 1001 GGAGTCAGAT ACCGAGACCC AGGACCATAG CCGGAAGACG GAGAGTGGCA 1051 GCGACCAGTC AGAGACCCCT GGGGCCCCGG TGCGGAGAGG GACCACCTAT 1101 GTGGAGCAGG TCCAGGAGGA GCTGGGGGAG CTGGGCGAGG CGTCCCAGGT 1151 GGAGACAGTG TCAGAGGAGA ACAAGAGTCT GATGTGGACC CTGCTGAAGC 1201 AGCTACGGCC AGGCATGGAC CTGTCCCGCG TGGTGCTACC CACGTTCGTA 1251 CTGGAGCCGC GCTCCTTCCT GAACAAGCTC TCCGACTACT ACTACCACGC 1301 AGACCTGCTC TCCAGGGCTG CGGTGGAGGA GGATGCCTAC AGCCGCATGA 1351 AGCTGGTGCT GCGGTGGTAC CTGTCTGGCT TCTACAAGAA GCCCAAGGGA 1401 ATCAAGAAGC CGTACAACCC CATCCTGGGG GAGACCTTCC GCTGCTGCTG 1451 GTTCCACCCG CAGACTGACA GCCGCACATT CTACATAGCA GAGCAGGTGT 1501 CCCACCACCC GCCCGTGTCT GCCTTCCACG TCAGCAACCG GAAGGACGGC 1551 TTCTGCATCA GTGGCAGCAT CACAGCCAAG TCCAGGTTTT ATGGGAACTC 1601 GCTGTCGGCG CTGCTGGACG GCAAAGCCAC GCTCACCTTC CTGAACCGAG 1651 CCGAGGATTA CACCCTTACC ATGCCCTACG CCCACTGCAA AGGAATCCTG 1701 TATGGCACGA TGACCCTGGA GCTGGGTGGG AAGGTCACCA TCGAGTGTGC 1751 GAAGAACAAC TTCCAGGCCC AGCTGGAATT CAAACTCAAG CCCTTCTTCG 1801 GGGGTAGCAC CAGCATCAAC CAGATCTCGG GAAAGATCAC GTCGGGAGAG 1851 GAAGTCCTGG CGAGCCTCAG TGGCCACTGG GACAGGGACG TGTTTATCAA 1901 GGAGGAAGGG AGCGGAAGCA GTGCGCTTTT CTGGACCCCG AGCGGGGAGG 1951 TCCGCAGACA GAGGCTGAGG CAGCACACGG TGCCGCTGGA GGAGCAGACG 2001 GAGCTGGAGT CCGAGAGGCT CTGGCAGCAC GTCACCAGGG CCATCAGCAA 2051 GGGCGACCAG CACAGGGCCA CACAGGAGAA GTTTGCACTG GAGGAGGCAC 2101 AGCGGCAGCG GGCCCGTGAG CGGCAGGAGA GCCTCATGCC CTGGAAGCCG 2151 CAGCTGTTCC ACCTGGACCC CATCACCCAG GAGTGGCACT ACCGATACGA 2201 GGACCACAGC CCCTGGGACC CCCTGAAGGA CATCGCCCAG TTTGAGCAAG 2251 ACGGGATCCT GCGGACCTTG CAGCAGGAGG CCGTGGCCCG CCAGACCACC 2301 TTCCTGGGCA GCCCAGGGCC CAGGCACGAG AGGTCTGGCC CAGACCAGCG 2351 GCTTCGCAAG GCCAGCGACC AGCCCTCCGG CCACAGCCAG GCCACGGAGA 2401 GCAGCGGATC CACGCCTGAG TCCTGCCCAG AGCTCTCAGA CGAGGAGCAG 2451 GATGGTGACT TTGTCCCTGG CGGTGAGAGC CCATGCCCTC GGTGCAGGAA 2501 GGAGGCGCGG CGGCTGCAGG CCCTGCACGA GGCCATCCTC TCCATCCGAG 2551 AGGCCCAGCA GGAGCTGCAC AGGCACCTCT CGGCCATGCT GAGCTCCACG 2601 GCACGGGCAG CACAGGCACC GACCCCAGGC CTCCTGCAGA GCCCCCGATC 2651 CTGGTTCCTG CTCTGCGTGT TCCTGGCGTG TCAGCTGTTC ATTAACCACA 2701 TCCTCAAATA GGAGCCCTGG GGGCAGAGCT CCTGGCCAGT CCCGAGCCCT 2751 CCCTCCCAGG CACCCAGCAC TTTAAGCCTG CTCCATGGAG GCAGAGAGGC 2801 CCGGCAAGCA CAGCCACTGT GACGGGGAGT CCAGGCGCAG GAGGGACCCG 2851 GGGCCACAAG GCGCTGCGGG CCCAGGTGTG CTGGGCCCCT CTCAGGGGCA 2901 CTGGCCTCTC TGCAGGGCCT TCCGCCCAGC GCTGGCCTTA ATGCTAAAGC 2951 CAAATGCAGC TTCTGCTGTG CGACGCACTC CTGGCCATCT TGCCGTGTCA 3001 CCCCCTGTCC GGCCTCCACT TGCCATGGGG GATGGATGGA TTTAGGGTGG 3051 GAGGGCCTGT GGGGGCCCTG GACAGTCACA CCCCAGCAGC AGTGAGTGGG 3101 CAGGTTTGGA GGAGCAGCCA GGGAGCCCCG AGTGGCCCAG GAGTCCCCCC 3151 ACACACAGAT GCATAGGCCT GCCTTCCGGA GACCCTGTCC ACATTGCCGG 3201 GACCACCCTG GTGGGGCCAC TGGTGGGTGC CAGGGACAGG TTAGGGCCAC 3251 TCTGGGGAAG GCATTTTGGT TTTTTATTCC ACGCTCTGCT GTTTGGATGG 3301 GAGCCCCACA GAGGCAGGTC CTGGAACCAC CCCACCCCCA CACCTGGACG 3351 CTCGCTCTGG TGGGGGCACA CGCAGGTGGA GGTGGTTGTG GGTGCAGGTG 3401 TGTGCAGGGG TGTGGGGGGC GCAGGGGTGT GGCTTAGCTG GCCCCGCACC 3451 CAGGCCGGGG AGGCTCAAGT TCGCCACTTT ACTCAGACCG ATGCACAGTC 3501 TTCCCATTTT ACACTTTTTT AATAAACATA ATTGCAATAT TTTAGGTGGG 3551 CTGCGAGCTG CAGTCAGCCT TCACGTCTGG CCTCAGTCCC CGTGTCAGTG 3601 CCGCTCTGCG TGTGCGTGTG CGCGTGTGTG AGCCTCTACA CATATATATA 3651 TGTACAGAGC CTTAAACCAC ATCGTGGCGG TGCCGTCTGA GCTGTAGCGG 3701 GTGGCTTTGT TTCCAGTTTT TGTACCCGTG TCCTTGTCTC CCCTCCTCCC 3751 CCATCTGGGG ATGTGTCTGT GTTCCACACC TTGAAATAAA CAGACACATA 3801 CGTGTTCTCT TAAAAAAAAA AAAAAAAA BLAST Results No BLAST result Medline entries 98315477: The pleckstπn homology domain of oxysterol-binding protein recognises a determinant specific to Golgi membranes . 98146266: A Drosophila homologue of oxysterol binding protein (OSBP) —implications for the role of OSBP. 98146266: A Drosophila homologue of oxysterol binding protein (OSBP) --implications for the role of OSBP. Peptide information for frame 3 ORF from 72 bp to 2708 bp; peptide length: 879 Category: strong similarity to known protein 1 MKEEAFLRRR FSLCPPSSTP QKVDPRKLTR NLLLSGDNEL YPLSPGKDME 51 PNGPSLPRDE GPPTPSSATK VPPAEYRLCN GSDKECVSPT ARVTKKETLK 101 AQKENYRQEK KRATRQLLSA LTDPSVVIMA DSLKIRGTLK SWTKLWCVLK 151 PGVLLIYKTP KVGQWVGTVL LHCCELIERP SKKDGFCFKL FHPLDQSVWA 201 VKGPKGESVG SITQPLPSSY LIFRAASESD GRCWLDALEL ALRCSSLLRL 251 GTCKPGRDGE PGTSPDASPS SLCGLPASAT VHPDQDLFPL NGSSLENDAF 301 SDKSERENPE ESDTETQDHS RKTESGSDQS ETPGAPVRRG TTYVEQVQEE 351 LGELGEASQV ETVSEENKSL MWTLLKQLRP GMDLSRVVLP TFVLEPRSFL 401 NKLSDYYYHA DLLSRAAVEE DAYSRMKLVL RWYLSGFYKK PKGIKKPYNP 451 ILGETFRCCW FHPQTDSRTF YIAEQVSHHP PVSAFHVSNR KDGFCISGSI 501 TAKSRFYGNS LSALLDGKAT LTFLNRAEDY TLTMPYAHCK GILYGTMTLE 551 LGGKVTIECA KNNFQAQLEF KLKPFFGGST SINQISGKIT SGEEVLASLS 601 GHWDRDVFIK EEGSGSSALF WTPSGEVRRQ RLRQHTVPLE EQTELESERL 651 WQHVTRAISK GDQHRATQEK FALEEAQRQR ARERQESLMP WKPQLFHLDP 701 ITQEWHYRYE DHSPWDPLKD IAQFEQDGIL RTLQQEAVAR QTTFLGSPGP 751 RHERSGPDQR LRKASDQPSG HSQATESSGS TPESCPELSD EEQDGDFVPG 801 GESPCPRCRK EARRLQALHE AILSIREAQQ ELHRHLSAML SSTARAAQAP 851 TPGLLQSPRS WFLLCVFLAC QLFINHILK BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphutel_19hl7, frame 3 TREMBL :CEZK1086_2 gene: "ZK1086.1"; Caenorhabditis elegans cosmid ZK1086, N = 1, Score = 1495, P = 2.7e-153 PIR:S25324 hypothetical protein YKR003w - yeast (Saccharomyces cerevisiae), N = 2, Score = 574, P = 8.5e-57 TREMBL:CEAF195_7 gene: "C32F10.1"; Caenorhabditis elegans cosmid C32F10., N = 1, Score = 588, P = 8.6e-57 PIR:S46796 hypothetical protein YKR003w homolog YHROOlw - yeast (Saccharomyces cerevisiae), N = 1, Score = 585, P = 1.9e-56 TREMBL :NCOSBP_l gene: "osbP"; product: "oxysterol-binding protein"; N.crassa mRNA for putative oxysterol-binding protein, N = 1, Score = 571, P = 7e-55 TREMBL: AB017026_1 product: "oxysterol-binding protein"; Mus musculus mRNA for oxysterol-b dmg protein, complete eds., N = 2, Score = 328, P = 3e-35 >TREMBL:CEZK1086_2 gene: "ZK1086.1"; Caenorhabditis elegans cosmid ZK1086 Length = 751 HSPs: Score = 1495 (224.3 bits), Expect = 2.7e-153, P = 2.7e-153 Identities = 327/663 (49%), Positives = 430/663 (64%) Query: 129 MADSLKIRGTLKSWTKLWCVLKPGVLLIYKTPKV—GQWVGTVLLHCCELIERPSKKDGF 186 MAD+LKIRG LK W + +CVLKPG+L++YK K G WVGTVLL+ CELIERPSKKDGF Sbjct: 1 MADTLKIRGALKRWNRYYCVLKPGLLILYKHKKADRGDWVGTVLLNHCELIERPSKKDGF 60 Query: 187 CFKLFHPLDQSVWAVKGPKGESVGSIT-QPLPSSYLIFRAASESDGRCWLDALELALRCS 245 CFKLFHP+D S+W +GP G+S GS T PL +S+LI RA S+ GRCW+DALEL+ +C+ Sbjct: 61 CFKLFHPMDMSIWGNRGPLGQSFGSFTLNPLNTSFLICRAPSDQAGRCWMDALELSFKCT 120 Query: 246 SLLRLGTCKPGRDGEPGTSPDASPSSLCGLPASATVHPDQDLFPLNGSSLENDAFΞDK-S 304 LL+ T D + G D+S + G + + D D G A Ξ+ + Sbjct: 121 GLLKK-TMNE-LDDKNG DSSMND—GQRDESRMSRDSD GDDTRELAVSETDA 168 Query: 305 ERENPEESDTETQDHSRKTESGSDQSETPGAPVRRGTT YVEQVQEELGELGEAΞQVE 361 E+ E D + +DH E G SET +R T ++ +E G G S E Sbjct: 169 EKHFQEIDDVQDEDH EDGK-MSETSDT-IREAFTESAWIPSPKEVFGPDG—SLTE 220 Query: 362 TVSEENKSLMWTLLKQLRPGMDLSRVVLPTFVLEPRSFLNKLSDYYYHADLLSRAAVEED 421 V EENKSL+WTLLKQ+RPGMDLS+VVLPTF+LEPRSFL KL+DYYYHADL+S A E D Sbjct: 221 EVGEENKSLIWTLLKQIRPGMDLSKVVLPTFILEPRSFLEKLADYYYHADLISEAVAEPD 280 Query: 422 AYSRMKLVLRWYLSGFYKKPKGIKKPYNPILGETFRCCWFHPQTDSRTFYIAEQVSHHPP 481 + R+ V +++LSGFYKKPKG+KKPYNPILGETFRC W HP S TFY+AEQVSHHPP Sbjct: 281 PFQRIVKVTKFFLSGFYKKPKGLKKPYNPILGETFRCKWEHPD-GSTTFYMAEQVSHHPP 339 Query: 482 VSAFHVSNRKDGFCISGSITAKSRFYGNSLSALLDGKATLTFLNRAEDYTLTMPYAHCKG 541 VS+ ++NRK GF ISG+I AKS++YGNSLSA+L GK LT LN E Y + +PYA+CKG Sbjct: 340 VSSLFITNRKAGFNISGTILAKSKYYGNSLSAILAGKLRLTLLNLGETYIVNLPYANCKG 399 Query: 542 ILYGTMTLELGGKVTIECAKNNFQAQLEFKLKPFFGGSTSINQISGKITSGEEVLASLSG 601 1+ GTMT+ELGG+V IEC K ++ L+FKLKP GG+ NQI G I G + LAS+ G Sbjct: 400 IMIGTMTMELGGEVNIECEKTGYRTTLDFKLKPMLGGA--YNQIEGΞIKYGSDRLASIEG 457 Query: 602 HWDRDVFIKEEGSGSSALFWTPSGEVRRQRLRQHTVPLEEQTELESERLWQHVTRAISKG 661 WD + IK G W P+ EV + RL ++ + ++EQ E ES +LW+HVT AIS Sbjct: 458 AWDGVIRIK—GPDGKKELWNPTPEVIKTRLPRYEINMDEQGEWESAKLWRHVTEAISNE 515 Query: 662 DQHRATQEKFALEEAQRQRARERQESLMPWKPQLFHLDPITQEWHYRYEDHSPWDPLKDI 721 DQ++AT+EK ALE QR RA+ S +P + + F ++ Y + D+ PWD Dl Sbjct: 516 DQYKATEEKTALENDQRARAK SGIPHETKFFKKQH-GDDYVYIHADYRPWDNNNDI 570 Query 722 AQFEQDGILRTLQQEAVAR--QTTFLGSPGPRHERSGPDQRLRKASDQPSGHSQATESSG 779 Q E + +++T+ + + + + LGS E S D+ + +P + + Sbjct 571 QQIENNYVVKTISRHSKRKTGNSEQLGSDNTS-EASESDEEVI EPKIKKKEIVPAK 625 Query 780 STPESCPELSDE 791 S P + PE++DE Sbjct 626 SKPIT-PEVADE 636 Pedant information for DKFZphutel_19hl7, frame 3 Report for DKFZphutel_19hl7.3 [LENGTH] 879 [MW] 98616.79 [pi] 7.29 [HOMOL] TREMBL:CEZK1086_2 gene: 'ZK1086.1"; Caenorhabditis elegans cosmid ZK1086 le-157 [ FUNCAT ] 01.06.16 lipid and fatty-acid binding [S. cerevisiae, YHROOlw] 3e-55 [FUNCAT] 01.06.01 lipid, fatty-acid and sterol biosynthesis [S. cerevisiae, YHROOlw] 3e-55 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YPL145C] 3e-23 [FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YPL145c] 3e-23 [FUNCAT] 04.05.01.07 chromatin modification [S. cerevisiae, YAR044w] 5e-20 [BLOCKS] BL00168F [BLOCKS] BL01013D Oxysterol-binding protein family proteins [BLOCKS] BL01013C Oxysterol-binding protein family proteins [BLOCKS] BL01013B Oxysterol-binding protein family proteins [BLOCKS] BL01013A Oxysterol-bmding protein family proteins [PIRKW] transmembrane protein le-19 [SUPFAM] pleckstrm repeat homology 8e-18 [SUPFAM] ankyrin repeat homology le-19 [SUPFAM] unassigned ankyrin repeat proteins le-19 [PROSITE] MYRISTYL 12 [PROSITE] CAMP_PHOSPHO_SITE 6 [PROSITE] OSBP 1 [PROSITE] CK2_PHOSPHO_SITE 21 [PROSITE] PROKAR_LIPOPROTEIN 1 [PROSITE] TYR_PHOSPHO_SITE 2 [PROSITE] PKC_PHOSPHO_SITE 20 [PROSITE] ASN_GLYCOSYLATION 3 [PFAM] PH (pleckstrm homology) domain [KW] TRANSMEMBRANE 1 [KW] LOW_COMPLEXITY 2.96 % [KW] COILED COIL 3.53 % SEQ MKEEAFLRRRFSLCPPSSTPQKVDPRKLTRNLLLSGDNELYPLSPGKDMEPNGPSLPRDE SEG PRD ccchhhhhhhhccccccccccccccccccccccccccccccccccccccccccccccccc COILS MEM SEQ GPPTPSSATKVPPAEYRLCNGSDKECVSPTARVTKKETLKAQKENYRQEKKRATRQLLSA SEG PRD cccccccccccccceeeecccccceeeeccccchhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCC MEM SEQ LTDPSVVIMADSLKIRGTLKSWTKLWCVLKPGVLLIYKTPKVGQWVGTVLLHCCELIERP SEG PRD hcccceeeecccccccccccccceeeeeeccceeeeecccccccceeeeecccccccccc COILS CCC MEM SEQ SKKDGFCFKLFHPLDQSVWAVKGPKGESVGΞITQPLPSSYLIFRAASESDGRCWLDALEL SEG PRD ccccceeeeecccccceeeeecccccceeecccccccceeeeeeehhhhhhhhhhhhhhh COILS MEM SEQ ALRCSSLLRLGTCKPGRDGEPGTSPDASPSSLCGLPASATVHPDQDLFPLNGSSLENDAF SEG PRD hhhhhhhhhhhhcccccccccccccccccccccccccccccccccccccccccccccccc COILS MEM SEQ SDKSERENPEESDTETQDHSRKTESGSDQSETPGAPVRRGTTYVEQVQEELGELGEASQV SEG xxxxxxxxxxxxx .... PRD cccccccccccccccccccccccccccccccccccccccccccchhhhhhhhhccccccc COILS MEM SEQ ETVSEENKSLMWTLLKQLRPGMDLSRVVLPTFVLEPRSFLNKLSDYYYHADLLSRAAVEE SEG PRD cccccccchhhhhhhhhhcccccceeeccceeeecccchhhhhhhhhccccccccccccc COILS MEM SEQ DAYSRMKLVLRWYLSGFYKKPKGIKKPYNPILGETFRCCWFHPQTDSRTFYIAEQVSHHP SEG PRD chhhhhhhhhhhhhhhcccccccccccccccccceeeeeecccccccceeeeeccccccc COILS MEM SEQ PVSAFHVSNRKDGFCISGSITAKSRFYGNSLSALLDGKATLTFLNRAEDYTLTMPYAHCK SEG PRD cceeeeecccccccccccccccccccccccccccccceeeeeeccccceeeeccccceee COILS MEM SEQ GILYGTMTLELGGKVTIECAKNNFQAQLEFKLKPFFGGSTSINQISGKITSGEEVLASLS SEG PRD eeeeeccccccccceeeeeccccccceeeecccccccccccceeeeeccccccceeeeec COILS MEM SEQ GHWDRDVFIKEEGSGSSALFWTPSGEVRRQRLRQHTVPLEEQTELESERLWQHVTRAISK SEG PRD cccccceeeeeccccceeeeeccccccccccccccccccchhhhhhhhhhhhhhhhhhhh COILS MEM SEQ GDQHRATQEKFALEEAQRQRARERQESLMPWKPQLFHLDPITQEWHYRYEDHSPWDPLKD SEG xxxxxxxxxxxxx PRD cchhhhhhhhhhhhhhhhhhhhhhhhhhccccccccccccccccceeeeccccccccchh COILS MEM SEQ IAQFEQDGILRTLQQEAVARQTTFLGSPGPRHERSGPDQRLRKASDQPSGHSQATESSGS SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhccccccccccccchhhhhcccccccccccccccccc COILS MEM SEQ TPESCPELSDEEQDGDFVPGGEΞPCPRCRKEARRLQALHEAILSIREAQQELHRHLSAML SEG PRD ccccccccccccccccccccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS MEM SEQ SSTARAAQAPTPGLLQSPRSWFLLCVFLACQLFINHILK SEG PRD hhhhhhhcccccccccccceeeeehhhhhhhhhhhhccc COILS MEM MMMMMMMMMMMMMMMMM . Prosite for DKFZphutel_19hl7.3 PS00001 80->84 ASN_GLYCOSYLATION PDOC00001 PS00001 291->295 ASN_GLYCOSYLATION PDOC00001 PS00001 367->371 ASN_GLYCOSYLATION PDOC00001 PS00004 9->13 CAMP_PHOSPHO_SITE PDOC00004 PS00004 26->30 CAMP_PHOSPHO_SITE PDOC00004 PS00004 95->99 CAMP_PHOSPHO_ΞITE PDOC00004 PΞ00004 111->115 CAMP_PHOSPHO_SITE PDOC00004 PS00004 338->342 CAMP_PHOSPHO_SITE PDOC00004 PS00004 762->766 CAMP_PHOSPHO_SITE PDOC00004 PS00005 82->85 PKC_PHOSPHO_SITE PDOC00005 PS00005 90->93 PKC_PHOSPHO_SITE PDOC00005 PS00005 94->97 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 98->101 PKC_PHOSPHO_SITE PDOC00005 PS00005 132->135 PKC_PHOSPHO_SITE PDOC00005 PS00005 138->141 PKC_PHOSPHO_SITE PDOC00005 PS00005 159->162 PKC_PHOSPHO_SITE PDOC00005 PS00005 181->184 PKC_PHOSPHO_SITE PDOC00005 PS00005 252->255 PKC PHOSPHO SITE PDOC00005 1313 13131313 13131313131313131313 1313 U 13 13 U 1313 13131013 U 13131313131313131313 1313131313 r O 13131 r. 2 c W OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT OT CO OT CO OT OT CO OT OT CO CO OT OT OT CO 2 2 O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o o σ o o o o O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O O J A j C> C0 ∞ CO ∞ ∞ C0 CO ∞ ∞ ∞ OT -J -J C O"ι CTl C^ CTl T> σt CJ^ σι Crι 3^ ^ DKFZphutel_19jll group: uterus derived DKFZphutel_19jll encodes a novel 708 amino acid protein with C-terminal similarity to several known proteins, such as human KIAA0231 or murine ras binding protein Sur8. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of uterus-specific genes . Strong similarity to KIAA0231, similarity to ras binding protein Sur8 EST AA854189 extendes the sequence (294 Bp) , with this sequence complete cDNA, Sequenced by AGOWA Locus : unknown Insert length: 2343 bp Poly A stretch at pos. 2323, polyadenylation signal at pos. 2295 1 GCTCCTGCTA ACCCCATCAC TGTGGAAATG AAAGGCCTGA AGACAGATTT 51 GGACCTTCAG CAGTACAGCT TTATAAATCA GATGTGTTAT GAGCGAGCCC 101 TCCACTGGTA TGCCAAGTAT TTCCCTTACC TTGTCCTCAT CCATACCCTG 151 GTCTTTATGC TCTGCAGTAA CTTTTGGTTC AAATTCCCTG GTTCCAGCTC 201 CAAAATAGAA CATTTCATCT CCATTCTGGG GAAGTGTTTT GACTCTCCTT 251 GGACCACACG GGCTTTATCT GAAGTGTCTG GGGAGGACTC AGAAGAAAAG 301 GACAACAGGA AGAACAACAT GAACAGGTCC AACACCATCC AATCTGGTCC 351 AGAAGGCAGC CTGGTCAACT CTCAGTCTTT AAAGTCCATT CCTGAGAAGT 401 TTGTAGTTGA TAAATCCACT GCAGGGGCTC TGGATAAAAA GGAAGGTGAG 451 CAGGCTAAGG CCTTATTTGA GAAGGTGAAG AAGTTCAGGC TGCATGTGGA 501 AGAAGGTGAT ATTCTATATG CCATGTATGT TCGCCAGACT GTACTTAAAG 551 TTATCAAATT CCTAATCATC ATTGCATATA ATAGTGCTCT GGTTTCCAAG 601 GTCCAGTTTA CAGTGGACTG TAATGTGGAC ATTCAGGACA TGACTGGATA 651 TAAAAACTTT TCTTGCAATC ATACCATGGC ACACTTGTTC TCAAAACTGT 701 CCTTTTGCTA TCTGTGCTTT GTTAGTATCT ATGGATTGAC GTGCCTTTAT 751 ACCTTATACT GGCTGTTCTA CCGTTCTCTA CGGGAATATT CCTTTGAGTA 801 TGTCCGTCAG GAGACTGGAA TTGATGATAT TCCAGATGTG AAAAATGACT 851 TTGCTTTTAT GCTTCATATG ATAGATCAGT ATGACCCTCT CTATTCCAAG 901 AGATTTGCAG TGTTCCTGTC TGAAGTCAGT GAAAACAAAT TAAAGCAGCT 951 GAACTTAAAT AACGAATGGA CTCCTGATAA ACTGAGGCAG AAGCTACAGA 1001 CAAATGCCCA TAATCGACTG GAATTGCCTC TTATCATGCT CTCTGGCCTT 1051 CCAGACACTG TTTTTGAAAT CACAGAGTTG CAATCTCTAA AACTTGAAAT 1101 CATTAAGAAC GTAATGATAC CAGCCACCAT TGCACAGCTA GACAATCTTC 1151 AAGAGCTCTC TCTGCACCAG TGTTCTGTCA AAATCCACAG TGCGGCGCTC 1201 TCTTTCCTGA AGGAAAACCT CAAGGTCTTG AGCGTCAAGT TTGATGACAT 1251 GAGGGAACTC CCCCCCTGGA TGTATGGGCT CCGAAATCTG GAAGAGCTGT 1301 ACCTAGTTGG CTCTCTAAGT CATGATATTT CCAGAAATGT CACCCTTGAG 1351 TCTCTGCGGG ATCTCAAAAG CCTTAAAATT CTCTCTATCA AAAGCAACGT 1401 TTCCAAAATC CCTCAGGCAG TGGTTGATGT TTCCAGCCAT CTCCAGAAGA 1451 TGTGCATACA TAATGATGGC ACCAAGCTGG TGATGCTCAA CAACTTAAAG 1501 AAGATGACCA ATCTGACAGA GCTGGAGCTG GTCCACTGTG ACCTGGAGCG 1551 TATTCCTCAT GCTGTGTTCA GCCTACTCAG CCTCCAGGAA TTGGACCTGA 1601 AGGAAAACAA TCTGAAATCT ATAGAAGAAA TCGTTAGCTT TCAGCACTTA 1651 AGAAAGTTGA CAGTGCTAAA ACTGTGGCAT AACAGCATCA CCTACATCCC 1701 AGAGCATATA AAGAAACTCA CCAGCCTGGA ACGCCTGTCC TTTAGTCACA 1751 ATAAAATAGA GGTGCTGCCT TCCCACCTCT TCCTATGCAA CAAGATCCGA 1801 TACTTGGACT TATCGTACAA TGACATTCGA TTTATCCCCC CTGAAATTGG 1851 AGTTCTACAA AGTTTACAGT ATTTTTCCAT CACATGTAAC AAAGTGGAAA 1901 GCCTTCCAGA TGAACTCTAC TTCTGCAAGA AACTTAAAAC TCTGAAGATT 1951 GGAAAAAACA GCCTATCTGT ACTTTCACCG AAAATTGGAA ATTTGCTATT 2001 TCTTTCCTAC TTAGATGTAA AAGGTAATCA CTTTGAAATC CTCCCTCCTG 2051 AACTGGGTGA CTGTCGGGCT CTGAAGCGAG CTGGTTTAGT TGTAGAAGAT 2101 GCTCTGTTTG AAACTCTGCC TTCTGACGTC CGGGAGCAAA TGAAAACAGA 2151 ATAACTTATT TTTCGTTAAA GTTTGACTGA AACACGCTTC TACCAAATAC 2201 AGTATAAATA ATTAGGTAGT CTTAATGCCT TTCCTATTTT TTTTTCCTTT 2251 TCACACAAAA TGTACACAAA GATCGCGTAA GGAGTATGTA TTTTTAATAA 2301 AAATTTAATT GTATTTTTTC AATATTAAAA AAAAAAAAAA AAA BLAST Results o BLAST result Medline entries 96421675: Characterization of densin-180, a new brain-specific synaptie protein of the O-sialoglycoprotem family. 98337190: SUR-8, a conserved Ras-bmding protein with leucine-rich repeats, positively regulates Ras-mediated signaling in C. elegans . Peptide information for frame 1 ORF from 28 bp to 2151 bp; peptide length: 708 Category: similarity to known protein Classification: Cell signaling/communication 1 MKGLKTDLDL QQYSFINQMC YERALHWYAK YFPYLVLIHT LVFMLCSNFW 51 FKFPGSΞSKI EHFISILGKC FDSPWTTRAL SEVSGEDSEE KDNRKNNMNR 101 SNTIQSGPEG SLVNSQSLKS IPEKFVVDKS TAGALDKKEG EQAKALFEKV 151 KKFRLHVEEG DILYAMYVRQ TVLKVIKFLI IIAYNSALVS KVQFTVDCNV 201 DIQDMTGYKN FSCNHTMAHL FSKLSFCYLC FVSIYGLTCL YTLYWLFYRS 251 LREYSFEYVR QETGIDDIPD VKNDFAFMLH MIDQYDPLYS KRFAVFLΞEV 301 SENKLKQLNL NNEWTPDKLR QKLQTNAHNR LELPLIMLSG LPDTVFEITE 351 LQSLKLEIIK NVMIPATIAQ LDNLQELSLH QCSVKIHSAA LSFLKENLKV 401 LSVKFDDMRE LPPWMYGLRN LEELYLVGSL SHDISRNVTL ESLRDLKSLK 451 ILSIKSNVSK IPQAVVDVSS HLQKMCIHND GTKLVMLNNL KKMTNLTELE 501 LVHCDLERIP HAVFSLLSLQ ELDLKENNLK SIEEIVSFQH LRKLTVLKLW 551 HNSITYIPEH IKKLTSLERL SFSHNKIEVL PSHLFLCNKI RYLDLSYNDI 601 RFIPPEIGVL QSLQYFSITC NKVESLPDEL YFCKKLKTLK IGKNSLSVLS 651 PKIGNLLFLS YLDVKGNHFE ILPPELGDCR ALKRAGLVVE DALFETLPSD 701 VREQMKTE BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphutel_19j 11, frame 1 TREMBL :HSD984_1 gene: "KIAA0231"; Human mRNA for KIAA0231 gene, partial eds., N = 1, Score = 1408, P = 4.5e-144 TREMBL :AF054827_1 gene: "soc-2"; product: "leucine-rich repeat protein SOC-2"; Caenorhabditis elegans leucine-rich repeat protein SOC-2 (soc-2) mRNA, complete eds., N = 1, Score = 304, P = 5.7e-24 TREMBL :RNU66707_1 product: "densm-180"; Rattus norvegicus densm-180 mRNA, complete eds., N = 1, Score = 311, P = 7.4e-24 TREMBL :AF068921_1 product: "Ras-bmding protein SUR-8"; Mus musculus Ras-bmding protein SUR-8 mRNA, complete eds., N = 1, Score = 302, P = l.le-23 >TREMBL:HSD984_1 gene: "KIAA0231"; Human mRNA for KIAA0231 gene, partial eds . Length = 476 HSPs: Score = 1408 (211.3 bits), Expect = 4.5e-144, P = 4.5e-144 Identities = 265/471 (56%), Positives = 361/471 (76%) Query: 237 LTCLYTLYWLFYRSLREYSFEYVRQETGIDDIPDVKNDFAFMLHMIDQYDPLYSKRFAVF 296 LT Y+L+W+ SL++YSFE +R+++ DIPDVKNDFAF+LH+ DQYDPLYSKRF++F Sbjct: 1 LTSSYSLWWMLRSSLKQYSFEALREKSNYSDIPDVKNDFAFILHLADQYDPLYSKRFSIF 60 Query: 297 LSEVSENKLKQLNLNNEWTPDKLRQKLQTNAHNRLELPLIMLSGLPDTVFEITELQSLKL 356 LSEVSENKLKQ+NLNNEWT +KL+ KL NA +++EL L ML+GLPD VFE+TE++ L L Sbjct: 61 LSEVSENKLKQINLNNEWTVEKLKSKLVKNAQDKIELHLFMLNGLPDNVFELTEMEVLSL 120 Query: 357 EIIKNVMIPATIAQLDNLQELSLHQCSVKIHSAALSFLKENLKVLSVKFDDMRELPPWMY 416 E+I V +P+ ++QL NL+EL ++ S+ + AL+FL+ENLK+L +KF +M ++P W++ Sb j ct: 121 ELIPEVKLPSAVSQLVNLKELRVYHSSLVVDHPALAFLEENLKILRLKFTEMGKIPRWVF 180 Query: 417 GLRNLEELYLVGSLSHDISRNVTLESLRDLKSLKILSIKSNVSKIPQAVVDVSSHLQKMC 476 L+NL+ELYL G + + + LE +DLK+L+ L +KS++S+IPQ V D+ LQK+ Sbjct: 181 HLKNLKELYLSGCVLPEQLSTMQLEGFQDLKNLRTLYLKSSLSRIPQVVTDLLPSLQKLS 240 Query: 477 IHNDGTKLVMLNNLKKMTNLTELELVHCDLERIPHAVFSLLSLQELDLKENNLKSIEEIV 536 + N+G+KLV+LNNLKKM NL LEL+ CDLERIPH++FSL +L ELDL+ENNLK++EEI+ Sbjct: 241 LDNEGSKLVVLNNLKKMVNLKSLELISCDLERIPHSIFSLNNLHELDLRENNLKTVEEII 300 Query: 537 SFQHLRKLTVLKLWHNSITYIPEHIKKLTSLERLSFSHNKIEVLPΞHLFLCNKIRYLDLS 596 SFQHL+ L+ LKLWHN+I YIP I L++LE+LS HN IE LP LFLC K+ YLDLS Sbjct: 301 SFQHLQNLSCLKLWHNNIAYIPAQIGALSNLEQLSLDHNNIENLPLQLFLCTKLHYLDLS 360 Query: 597 YNDIRFIPPEIGVLQSLQYFSITCNKVESLPDELYFCKKLKTLKIGKNSLSVLSPKIGNL 656 YN + FIP El L +LQYF++T N +E LPD L+ CKKL+ L +GKNSL LSP +G L Sbjct: 361 YNHLTFIPEEIQYLSNLQYFAVTNNNIEMLPDGLFQCKKLQCLLLGKNSLMNLSPHVGEL 420 Query: 657 LFLSYLDVKGNHFEILPPELGDCRALKRAGLVVEDALFETLPSDVREQMKT 707 L++L++ GN+ E LPPEL C++LKR L+VE+ L TLP V E+++T Sbjct: 421 ΞNLTHLELIGNYLETLPPELEGCQSLKRNCLIVEENLLNTLPLPVTERLQT 471 Pedant information for DKFZphutel_19jll, frame 1 Report for DKFZphutel_19j 11.1 [LENGTH] 708 [MW] 81812.82 [pi] 7.55 [HOMOL] TREMBL:HSD984_1 gene: "KIAA0231"; Human mRNA for KIAA0231 gene, partial eds. le-149 [FUNCAT] 30.02 organization of plasma membrane [S. cerevisiae, YJL005w] 3e-17 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YJL005w] 3e-17 [FUNCAT] 10.04.03 second messenger formation [S. cerevisiae, YJL005w] 3e-17 [FUNCAT] 01.03.10 metabolism of cyclic and unusual nucleotides [Ξ. cerevisiae, YJL005w] 3e-17 [ FUNCAT ] 03.10 sporulation and germination [S. cerevisiae, YJL005w] 3e-17 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YKL193c] 3e-09 [FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylation, farnesylation and processing) [S. cerevisiae, YKL193c] 3e-09 [FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YAL021c] 9e-08 [FUNCAT] 01.05.04 regulation of carbohydrate utilization [S. cerevisiae, YAL021c] 9e-08 [ FUNCAT ] 01.01.04 regulation of amino-acid metabolism [S. cerevisiae, YAL021c] 9e-08 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YOR353c] 3e-07 [BLOCKS] BL00868F [BLOCKS] BL00985B Spermadhesins family proteins [EC] 3.4.17.3 Lysine carboxypeptidase le-08 [EC] 4.6.1.1 Adenylate cyclase 3e-18 [PIRKW] blocked amino end le-10 [PIRKW] phosphotransferase le-09 [PIRKW] nucleus 6e-08 [PIRKW] duplication 3e-18 [PIRKW] platelet le-10 [PIRKW] tandem repeat 7e-16 [PIRKW] keratan sulfate 7e-07 [PIRKW] metallo-carboxypeptidase le-08 [PIRKW] transmembrane protein le-10 [PIRKW] serme/threonine-specific protein kinase le-09 [PIRKW] autophosphorylation le-09 [PIRKW] cartilage 7e-07 [PIRKW] connective tissue 7e-07 [PIRKW] magnesium le-09 [PIRKW] cAMP biosynthesis 3e-18 [PIRKW] ATP le-09 [PIRKW] receptor le-09 [PIRKW] leucine zipper 3e-13 [PIRKW] glycoprotein 5e-12 [PIRKW] extracellular matrix 7e-07 [PIRKW] chondro tin sulfate proteoglycan 7e-07 [PIRKW] cell adhesion le-08 [PIRKW] hydrolase le-08 [PIRKW] sulfoprotein 7e-07 [PIRKW] membrane protein le-08 [PIRKW] phosphorus-oxygen lyase 3e-18 [PIRKW] collagen binding 7e-07 [SUPFAM] leucine-rich alpha-2-glycoprotem repeat homology 3e-21 [SUPFAM] chaoptm le-08 [SUPFAM] gelsolm repeat homology 3e-21 [SUPFAM] protein kinase homology le-09 [SUPFAM] protein kinase Xa21 le-09 [SUPFAM] fibromodulin 4e-12 [SUPFAM] yeast adenylate cyclase catalytic domain homology 3e-18 [SUPFAM] yeast adenylate cyclase 3e-18 [KW] TRANSMEMBRANE 3 [KW] LOW_COMPLEXITY 1.41 % SEQ MKGLKTDLDLQQYSFINQMCYERALHWYAKYFPYLVLIHTLVFMLCSNFWFKFPGSSSKI SEG PRD ccccchhhhhhhhhhhhhhhhhhhhhhhhhhccchhhhhhhhhhhccceeeeccccccee MEM MMMMMMMMMMMMMMMMM SEQ EHFISILGKCFDSPWTTRALSEVSGEDSEEKDNRKNNMNRSNTIQΞGPEGSLVNSQSLKS SEG PRD eeeeeeeecccccccceeeeecccccccccccccccccccccccccccccceeeeccccc MEM SEQ IPEKFVVDKSTAGALDKKEGEQAKALFEKVKKFRLHVEEGDILYAMYVRQTVLKVIKFLI SEG PRD cccceeecccccccccchhhhhhhhhhhhhhhhhhhhcccceeeehhhhhhhhhhhhhhh MEM MMMMMMMMM SEQ IIAYNSALVSKVQFTVDCNVDIQDMTGYKNFSCNHTMAHLFSKLSFCYLCFVSIYGLTCL SEG PRD hhhhcchhhhheeeeeccccccccccccccccccchhhhhhhhheeeeeeeeeeccceee MEM MMMMMMMM MMMMMMMMMMMMMMMMM SEQ YTLYWLFYRSLREYΞFEYVRQETGIDDIPDVKNDFAFMLHMIDQYDPLYSKRFAVFLSEV SEG PRD hhhhhhhhhhhhhhhhhhhhhhccccccccccchhhhhhhhhhcccchhhhhhhhhhhhh MEM SEQ SENKLKQLNLNNEWTPDKLRQKLQTNAHNRLELPLIMLSGLPDTVFEITELQSLKLEIIK SEG .. xxxxxxxxxx PRD hhhhhhhhhccccccccchhhhhhhhhhhhhhhhhhhcccccccchhhhhhhhhhhhhhh MEM SEQ NVMIPATIAQLDNLQELSLHQCSVKIHSAALSFLKENLKVLSVKFDDMRELPPWMYGLRN SEG PRD hccccccchhhhhhhhhhhhccccccccccccchhhhhhhhhhccccccccccccchhhh MEM SEQ LEELYLVGSLSHDIΞRNVTLESLRDLKSLKILSIKSNVSKIPQAVVDVSSHLQKMCIHND SEG PRD hhhhhhccccccccccccccchhhhhhhhhhhhcccccccccccchhhhhhhhhhhcccc MEM SEQ GTKLVMLNNLKKMTNLTELELVHCDLERIPHAVFSLLSLQELDLKENNLKSIEEIVSFQH SEG PRD ceeeecccccccchhhhhhhhhccccccccccchhhhhhhhhhhccccccccccccccch MEM SEQ LRKLTVLKLWHNSITYIPEHIKKLTSLERLSFSHNKIEVLPSHLFLCNKIRYLDLSYNDI SEG PRD hhhhhhhcccccceeecccccchhhhhheeeccccceeecccccchhhhhhhhhhccccc MEM SEQ RFIPPEIGVLQSLQYFSITCNKVESLPDELYFCKKLKTLKIGKNSLSVLSPKIGNLLFLS SEG PRD cccccccchhhhhhhhhhhccccccccccccchhhhhcccccccceeecccccccchhhh MEM SEQ YLDVKGNHFEILPPELGDCRALKRAGLVVEDALFETLPSDVREQMKTE SEG PRD hhhccccccccccccchhhhhhhhheeeeccccccccccccccccccc MEM (No Prosite data available for DKFZphutel_19j 11.1) (No Pfam data available for DKFZphutel_19j 11.1) DKFZphutel_lι2 group: transcription factor DKFZphutel_lι2 encodes a novel 594 amino acid protein similar to signal transducing proteins. The protein contains 2 WD-40 repeats, which is typical for the beta-transducin subunit of G- proteins . In addition, the protein contains a C3HC4 zinc finger and a leucine zipper. The beta subunits seem to be required for the replacement of GDP by GTP as well as for membrane anchoring and receptor recognition. Due to the zmc finger the novel protein seems to be a new molecule involved in signal transduction and transcription. The new protein can find application in modulating/blocking gene expression of genes controlled by this molecule. similarity to Dictostelium myosin heavy chain kinase complete cDNA, complete eds, EST hits [PFAM] Zinc finger, C3HC4 type (RING fmger) [PFAM] WD domain, G-beta repeats [SCOP] dltbgc_ 2.46.3.1.1 betal-subumt of the signal-transducing G protei 3e-07 Sequenced by BMFZ Locus: /map="16pl3.3" Insert length: 3584 bp Poly A stretch at pos. 3555, polyadenylation signal at pos. 3537 1 GGGCGGGAGG TGCTTCCCAA GGACCGTAGA TGCCTCTCTA GAGCATGAGC 51 TCAGGCAAGA GTGCCCGCTA CAACCGCTTC TCCGGGGGGC CCAGCAATCT 101 TCCCACCCCA GACGTCACCA CAGGGACCAG AATGGAAACG ACCTTCGGAC 151 CCGCCTTTTC AGCCGTCACC ACCATCACAA AAGCTGACGG GACCAGCACC 201 TACAAGCAGC ACTGCAGGAC AGCATGCCCC CCATCAGCAC TCCCCGCCGC 251 TCCGACTCCG CCATCTCTGT CCGCTCCCTG CACTCAGAGT CCAGCATGTC 301 TCTGCGCTCC ACATTCTCAC TGCCCGAGGA GGAGGAGGAG CCGGAGCCAC 351 TGGTGTTTGC GGAGCAGCCC TCGGTGAAGC TGTGCTGTCA GCTCTGCTGC 401 AGCGTCTTCA AAGACCCCGT GATCACCACG TGTGGGCACA CGTTCTGTAG 451 GAGATGCGCC TTGAAGTCAG AGAAGTGTCC CGTGGACAAC GTCAAACTGA 501 CCGTGGTGGT GAACAACATC GCGGTGGCCG AGCAGATCGG GGAGCTCTTC 551 ATCCACTGCC GGCACGGCTG CCGGGTAGCG GGCAGCGGGA AGCCCCCCAT 601 CTTTGAGGTG GACCCCCGAG GGTGCCCCTT CACCATCAAG CTCAGCGCCC 651 GGAAGGACCA CGAGGGCAGC TGTGACTACA GGCCTGTGCG GTGTCCCAAC 701 AACCCCAGCT GCCCCCCGCT GCTCAGGATG AACCTGGAGG CCCACCTCAA 751 GGAGTGCGAG CACATCAAAT GCCCCCACTC CAAGTACGGG TGCACGTTCA 801 TCGGGAACCA GGACACTTAC GAGACCCACC TGGAGACTTG CCGCTTCGAG 851 GGCCTGAAGG AGTTTCTGCA GCAGACGGAT GACCGCTTCC ACGAGATGCA 901 CGTGGCTCTG GCCCAGAAGG ACCAGGAGAT CGCCTTCCTG CGCTCCATGC 951 TGGGAAAGCT CTCGGAGAAG ATCGACCAGC TAGAGAAGAG CCTGGAGCTC 1001 AAGTTTGACG TCCTGGACGA AAACCAGAGC AAGCTCAGCG AGGACCTCAT 1051 GGAGTTCCGG CGGGACGCAT CCATGTTAAA TGACGAGCTG TCCCACATCA 1101 ACGCGCGGCT GAACATGGGC ATCCTAGGCT CCTACGACCC TCAGCAGATC 1151 TTCAAGTGCA AAGGGACCTT TGTGGGCCAC CAGGGCCCTG TGTGGTGTCT 1201 CTGCGTCTAC TCCATGGGTG ACCTGCTCTT CAGTGGCTCC TCTGACAAGA 1251 CCATCAAGGT GTGGGACACA TGTACCACCT ACAAGTGTCA GAAGACACTG 1301 GAGGGCCATG ATGGCATCGT GCTGGCTCTC TGCATCCAGG GGTGCAAACT 1351 CTACAGCGGC TCTGCAGACT GCACCATCAT TGTGTGGGAC ATCCAGAACC 1401 TGCAGAAGGT GAACACCATC CGGGCCCATG ACAACCCGGT GTGCACGCTG 1451 GTCTCCTCAC ACAACGTGCT CTTCAGCGGC TCCCTGAAGG CCATCAAGGT 1501 CTGGGACATC GTGGGCACTG AGCTGAAGTT GAAGAAGGAG CTCACAGGCC 1551 TCAACCACTG GGTGCGGGCC CTGGTGGCTG CCCAGAGCTA CCTGTACAGC 1601 GGCTCCTACC AGACAATCAA GATCTGGGAC ATCCGAACCC TTGACTGCAT 1651 CCACGTCCTG CAGACGTCTG GTGGCAGCGT CTACTCCATT GCTGTGACAA 1701 ATCACCACAT TGTCTGTGGC ACCTACGAGA ACCTCATCCA CGTGTGGGAC 1751 ATTGAGTCCA AGGAGCAGGT GCGGACCCTC ACGGGCCACG TGGGCACCGT 1801 GTATGCCCTG GCGGTCATCT CGACGCCAGA CCAGACCAAA GTCTTCAGTG 1851 CATCCTACGA CCGGTCCCTC AGGGTCTGGA GTATGGACAA CATGATCTGC 1901 ACGCAGACCC TGCTGCGTCA CCAGGGCAGT GTCACCGCGC TGGCTGTGTC 1951 CCGGGGCCGA CTCTTCTCAG GGGCTGTGGA TAGCACTGTG AAGGTTTGGA 2001 CTTGCTAACA GGATCCAGGC CAGGCTGTGG TTTCCCCTGA ACCAGCCCTG 2051 GACCTTTCTG AGCCAGGCTG GCCACATGGG GTGGTCTCGG GGTTTCTGCC 2101 TGCCCCGTGG GCATAGGTGG ACAGGCTCTG GCAGCCGGGC AGTGCCCTCC 2151 CCGTCCCATG CTCGGCGAGC CTCCCTCTAC TCGGCACTGT CCTTGCTGCC 2201 CAGCCCCTCT CTGGGTGCCA GGTACGACGC TTGCCCCGGC CCACCCTCCA 2251 TCCCCACCCT CCATCCCCAC CCTAGATGGA GCGAGGGCCT TTTTACTCAC 2301 CTTTTCTACC GTTTTTAGAC TGTATGTAGA TTTGGTTACC TCCTGGTTGA 2351 AATAAATGCT CCACAGACTG TGGCTGTGAG TGGGGACAGC TCCTCGGGAC 2401 AAGGGGGCTG TGTGTGGCCT TGAGGTTGGT GTGCACAGGC ACTGGCTGCT 2451 GTGAGTGGGG GGGCATGGGG CAGTTTCCTT TGGTGGACCC CAGGACTTCG 2501 GCCCACTCCG GGGCCTCCCC TCCCTGCTAG GAGGCAACTC GTCACACCCA 2551 AGCTGCTGGC CTCCAGTCCC ATCTCCCCCA ACACATGTGC CCCCAAAAAG 2601 TGAGCCAGGC ACCTCTGTTT CCTGCTGTTT ATTGACAGCC GACGGCAGCG 2651 CCTTGCCCAG ACCTCCCCTG CCCACCTGCT GGAGCCCAGC CTGTGCCGCC 2701 CTCTGAGGAG AGGCCTGGGG GGACAGCTGG GCACGTCCAC TCGCAGGGAA 2 751 ACACGGGGTG AGACAGCAGG AAGGGGCCCT GCACGCCGGG ACGCCACCTC 2801 CGCCAGCCGC CTCCACCCGC CCCACACCAC AATCGCTGGT TTTCGGCATT 2851 TTTTAAATTT TTTTTTTAAG AAACGTCAAA GTTGTGCCCA ACACTGTGGA 2901 TCAGCAAACA CGATAGAGGA GACCAGTCAG TACTTCTTGG AGGGGGCAGG 2951 AGGAGAGAGG AAAAGGGAGG GCGAGAATGA CCACACAACA CAGCCTTGGA 3001 CCATGAGCAG AAGCGTCCGT GGGAACTCCA CTGGGGTGGA TGGGCTGCCT 3051 GCACAGCCCC TGGAGAGGGG GCCAGGCACA CCCTCAGAGG AGCTGCAAGC 3101 CCGTGGCCTG GCCTGCTACA TGCCCTGCTT CCACGTGGCT GCCACGCTGA 3151 CACACCCACA TTCACCAAAC CCACCCGCGC CCTGGGACGC AGCCACGCCA 3201 GGAGGAGGAC ACGGCCGCCG AGAGCAAGGC ACAACCTCGA GTTCTTGGGG 3251 CGCAGAGAAC TTAGGAGAGA AGCACGGAGG AGCCCCCGGC AGAGCACCCG 3301 CCCCCGGGCC CCAGCCTTCC ACCTGTGCTA GCAGCCTGGG GCCTCCACTC 3351 TGGCCGGAGG AAGGACCGCA GGCAGACAGC CTGGGCCTCT AACAGCTTTT 3401 GTCCGGAGCT AGACTTCGTG TCCTTTCAGT TGGTAAATGG TTTTCTATAG 3451 AATCAATAAT ATTTCTTTCT TTAAATATAT ATTTGTTAAA GTTATACCTT 3501 TTTGTTTCTC TGGGGAAATC CGCCTCAGCT CATTCCCAAT AAATTAATAC 3551 TCTTGATAAA AAAAAAAAAA AGAAAAAAAA AAAA BLAST Results Entry HSBE from database EMBL: Homo sapiens (clone exon trap d5) chromosome 16pl3.3 gene, exon. Score = 2375, P = 7. le-101, identities = 475/475 Entry HSBD from database EMBL: Homo sapiens (clone exon trap d32) chromosome 16pl3.3 gene, exon. Score = 876, P = 3.0e-31, identities = 176/177 Medl e entries 95122486: Structural analysis of myosin heavy chain kinase A from Dictyostelium. Evidence for a highly divergent protein kinase domain, an ammo-terminal coiled-coil domain, and a domain homologous to the beta-subumt of heterotπmeric G proteins . 96149460: Dictyostelium myosin heavy chain kinase A regulates myosin localization during growth and development. 97277316: Identification of a protein kinase from Dictyostelium with homology to the novel catalytic domain of myosin heavy chain kinase A. 96009891: A gene responsible for vegetative incompatibility in the fungus Podospora anser a encodes a protein with a GTP-bindmg motif and G beta homologous domain. Peptide information for frame 2 ORF from 224 bp to 2005 bp; peptide length: 594 Category: similarity to known protein Prosite motifs: ZINC_FINGER_C3HC4 (70-80) LEUCINE_ZIPPER (436-458) LEUCINE_ZIPPER (436-458) G_BETA_REPEATS (335-355) G BETA REPEATS (376-391) 1 MPPISTPRRS DSAISVRSLH ΞESSMSLRST FSLPEEEEEP EPLVFAEQPS 51 VKLCCQLCCS VFKDPVITTC GHTFCRRCAL KSEKCPVDNV KLTVVVNNIA 101 VAEQIGELFI HCRHGCRVAG SGKPPIFEVD PRGCPFTIKL SARKDHEGSC 151 DYRPVRCPNN PSCPPLLRMN LEAHLKECEH IKCPHSKYGC TFIGNQDTYE 201 THLETCRFEG LKEFLQQTDD RFHEMHVALA QKDQEIAFLR SMLGKLSEKI 251 DQLEKSLELK FDVLDENQSK LSEDLMEFRR DASMLNDELS HINARLNMGI 301 LGSYDPQQIF KCKGTFVGHQ GPVWCLCVYS MGDLLFSGSS DKTIKVWDTC 351 TTYKCQKTLE GHDGIVLALC IQGCKLYSGS ADCTIIVWDI QNLQKVNTIR 401 AHDNPVCTLV SSHNVLFSGS LKAIKVWDIV GTELKLKKEL TGLNHWVRAL 451 VAAQSYLYSG SYQTIKIWDI RTLDCIHVLQ TSGGSVYSIA VTNHHIVCGT 501 YENLIHVWDI ESKEQVRTLT GHVGTVYALA VISTPDQTKV FSASYDRSLR 551 VWSMDNMICT QTLLRHQGSV TALAVSRGRL FSGAVDSTVK VWTC BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphutel_lι2, frame 2 SWISSPROT :KMHB_DICDI MYOSIN HEAVY CHAIN KINASE B (EC 2.7.1.129) (MHCK B)., N = 1, Score = 419, P = 3.6e-37 SWISSPROT :HETl_PODAN VEGETATIBLE INCOMPATIBILITY PROTEIN HET-E-1., N = 1, Score = 392, P = 3.1e-33 SWISSPROT :YDJ5_SCHPO HYPOTHETICAL 67.1 KD TRP-ASP REPEATS CONTAINING PROTEIN C57A10.05C IN CHROMOSOME I., N = 1, Score = 357, P = 4.1e-30 TREMBL:AF032878_1 gene: "slimb"; product: "Slimb"; Drosophila melanogaster Slimb (slimb) mRNA, complete eds., N = 1, Score = 347, P = 1.7e-29 >SWISΞPROT:KMHB_DICDI MYOSIN HEAVY CHAIN KINASE B (EC 2.7.1.129) (MHCK B) . Length = 732 HSPs: Score = 419 (62.9 bits), Expect = 3.6e-37, P = 3.6e-37 Identities = 96/268 (35%), Positives = 158/268 (58%) Query: 325 CLCVYSMGDLLFSGSSDKTIKVWD-TCTTYKCQKTLEGHDGIVLALCIQGCKLYSGSADC 383 C+C +LLF+G SD +I+V+D +C +TL+GH+G V ++C L+SGS+D Sbjct: 467 CIC DNLLFTGCSDNSIRVYDYKSQNMECVQTLKGHEGPVESICYNDQYLFSGSSDH 522 Query: 384 TIIVWDIQNLQKVNTIRAHDNPVCTLVSSHNVLFSGSL-KAIKVWDIVGTELKLKKELTG 442 +1 VWD++ L+ + T+ HD PV T++ + LFSGS K IKVWD+ L+ K L Sbjct: 523 SIKVWDLKKLRCIFTLEGHDKPVHTVLLNDKYLFSGSSDKTIKVWDL—KTLECKYTLES 580 Query: 443 LNHWVRALVAAQSYLYSGSY-QTIKIWDIRTLDCIHVLQTΞGGSVYSIAVTNHHIVCGTY 501 V+ L + YL+SGS +TIK+WD++T C + L+ V +1 + ++ G+Y Sbjct: 581 HARAVKTLCISGQYLFSGSNDKTIKVWDLKTFRCNYTLKGHTKWVTTICILGTNLYSGSY 640 Query: 502 ENLIHVWDIESKEQVRTLTGHVGTVYALAVISTPDQTKVFSASYDRSLRVWSMDNMICTQ 561 + I VW+++S E TL GH V + + D+ +F+AS D ++++W ++ + C Sbjct: 641 DKTIRVWNLKSLECSATLRGHDRWVEHMVIC DKL-LFTASDDNTIKIWDLETLRCNT 696 Query: 562 TLLRHQGSVTALAVSRGR—LFSGAVDSTVKVW 592 TL H +V LAV + + S + D +++VW Sbjct: 697 TLEGHNATVQCLAVWEDKKCVISCSHDQSIRVW 729 Score = 415 (62.3 bits). Expect = 1.2e-36, P = 1.2e-36 Identities = 113/303 (37%), Positives = 166/303 (54%) Query: 255 KSLEL-KFDVLDENQSKLSEDLMEFRRDASMLNDEL-SHINARLNMGILGS YD 305 KS++L K ++L N+ K S +L + ++ + SH+ N+ G YD Sbjct: 427 KSIDLEKPEILINNKKKESINLETIKLIETIKGYHVTSHLCICDNLLFTGCSDNSIRVYD 486 Query: 306 -PQQIFKCKGTFVGHQGPVWCLCVYSMGDLLFSGSΞDKTIKVWDTCTTYKCQKTLEGHDG 364 Q +C T GH+GPV +C Y+ LFSGSSD +IKVWD +C TLEGHD Sbjct: 487 YKSQNMECVQTLKGHEGPVESIC-YN-DQYLFSGSSDHSIKVWDL-KKLRCIFTLEGHDK 543 Query: 365 IVLALCIQGCKLYSGSADCTIIVWDIQNLQKVNTIRAHDNPVCTLVSSHNVLFSGSL-KA 423 V + + L+SGS+D TI VWD++ L+ T+ +H V TL S LFSGS K Sbjct: 544 PVHTVLLNDKYLFSGSSDKTIKVWDLKTLECKYTLESHARAVKTLCISGQYLFSGSNDKT 603 Query: 424 IKVWDIVGTELKLKKELTGLNHWVRALVAAQSYLYSGSY-QTIKIWDIRTLDCIHVLQTS 482 IKVWD+ + L G WV + + LYSGSY +TI++W++++L+C L+ Sbjct: 604 IKVWDL—KTFRCNYTLKGHTKWVTTICILGTNLYSGSYDKTIRVWNLKSLECSATLRGH 661 Query: 483 GGSVYSIAVTNHHIVCGTYENLIHVWDIESKEQVRTLTGHVGTVYALAVISTPDQTKVFS 542 V + + + + + +N I +WD+E+ TL GH TV LAV D+ V S Sbjct: 662 DRWVEHMVICDKLLFTASDDNTIKIWDLETLRCNTTLEGHNATVQCLAVWE—DKKCVIS 719 Query: 543 ASYDRSLRVW 552 S+D+S+RVW Sbjct: 720 CSHDQSIRVW 729 Score = 262 (39.3 bits), Expect = 3.2e-19, P = 3.2e-19 Identities == 60/184 (32%), Positives = 109/184 (59%) Query: 352 TYKCQKTLEGHDGIVLALCIQGCKLYSGSADCTIIVWDI--QNLQKVNTIRAHDNPVCTL 409 T K +T++G+ + LCI L++G +D +1 V+D QN++ V T++ H+ PV ++ Sbjct: 450 TIKLIETIKGYH-VTSHLCICDNLLFTGCSDNSIRVYDYKSQNMECVQTLKGHEGPVESI 508 Query: 410 VSSHNVLFSGSLK-AIKVWDIVGTELKLKKELTGLNHWVRALVAAQSYLYSGSY-QTIKI 467 + LFSGS +IKVWD+ +L+ L G + V ++ YL+SGS +TIK+ Sbjct: 509 CYNDQYLFSGSSDHSIKVWDL--KKLRCIFTLEGHDKPVHTVLLNDKYLFSGSSDKTIKV 566 Query: 468 WDIRTLDCIHVLQTSGGSVYSIAVTNHHIVCGTYENLIHVWDIESKEQVRTLTGHVGTVY 527 WD++TL+C + L++ +V ++ ++ ++ G+ + I VWD+++ TL GH V Sbjct: 567 WDLKTLECKYTLESHARAVKTLCIΞGQYLFSGSNDKTIKVWDLKTFRCNYTLKGHTKWVT 626 Query: 528 ALAVIST 534 + ++ T Sbjct: 627 TICILGT 633 Score : = 173 (26.0 bits), Expect = 1.7e-09, P = 1.7e-09 Identities ; . 43/118 (36%), Positives = 65/118 (55%) Query: 310 FKCKGTFVGHQGPVWCLCVYSMGDLLFSGSSDKTIKVWDTCTTYKCQKTLEGHDGIVLAL 369 F+C T GH V +C+ +G L+SGS DKTI+VW+ + +C TL GHD V + Sbjct: 612 FRCNYTLKGHTKWVTTICI—LGTNLYSGSYDKTIRVWNL-KSLECSATLRGHDRWVEHM 668 Query: 370 CIQGCKLYSGSADCTIIVWDIQNLQKVNTIRAHDNPV-CTLVSSH —VLFSGSLKAIKV 426 I L++ S D TI +WD++ L+ T+ H+ V C V V+ ++I+V Sbjct: 669 VICDKLLFTASDDNTIKIWDLETLRCNTTLEGHNATVQCLAVWEDKKCVISCSHDQSIRV 728 Query: 427 W 427 W Sbjct: 729 W 729 Pedant information for DKFZphutel_lι2, frame 2 Report for DKFZphutel_lι2.2 [LENGTH] 594 [MW] 66541.94 [pi] 6.64 [HOMOL] SWISSPROT :KMHB DICDI MYOSIN HEAVY CHAIN KINASE B (EC 2.7.1.129) (MHCK B) . 3e-37 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YIL046w] 5e-21 [FUNCAT] 06.13.01 cytoplasmic degradation [S. cerevisiae, YIL046w] 5e-21 [FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YIL046w] 5e-21 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YIL046w] 5e-21 [FUNCAT] 01.01.04 regulation of amino-acid metabolism [S. cerevisiae, YIL046w] 5e-21 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YCR072c beta-transducm family] 2e-15 [FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YFL009w] le-14 [FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YFL009w] le-14 [FUNCAT] 03.10 sporulation and germination [S. cerevisiae, YFL009w] le-14 [FUNCAT] 03.16 dna synthesis and replication [Ξ. cerevisiae, YFL009w] le-14 [FUNCAT] 30.09 organization of intracellular transport vesicles [S. cerevisiae, YDL145C] le-13 [FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YDL145c] le-13 [FUNCAT] 04.05.03 mrna processing (splicing) [S. cerevisiae, YPR178w] 2e-ll [FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YPR178w] 2e-ll [FUNCAT] 04.05.01.01 general transcription activities [S. cerevisiae, YBR198c TAF90 - TFIID subunit] 3e-ll [FUNCAT] 03.13 meiosis [S. cerevisiae, YLR129w] 8e-09 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YCR057c] 2e-07 [FUNCAT] 03.25 cytokinesis [S. cerevisiae, YCR057c] 2e-07 [FUNCAT] 02.16 fermentation [S. cerevisiae, YMR116c] 5e-07 [FUNCAT] 05.04 translation (initiation, elongation and termination) [S. cerevisiae, YMR116C] 5e-07 [FUNCAT] 06.13 proteolysis [S. cerevisiae, YGL003c] 3e-06 [FUNCAT] 03.01 cell growth [S. cerevisiae, YKL021c] 2e-04 [FUNCAT] 01.03.07 deoxyribonucleotide metabolism [S. cerevisiae, YOR269w] 2e-04 [FUNCAT] 30.02 organization of plasma membrane [S. cerevisiae, YOR212w] 0.001 [ FUNCAT ] 10.05.07 g-proteins [S. cerevisiae, YOR212w) 0.001 [FUNCAT] 03.07 pheromone response, mating-type determination, sex-specific proteins [S. cerevisiae, YOR212w] 0.001 [BLOCKS] BL00678 [BLOCKS] BL00518 Zinc finger, C3HC4 type, proteins [SCOP] dltbgd_ 2.46.3.1.1 betal-subunit of the signal-transducmg 3e-10 [EC] 2.7.1.129 Myosin-heavy-cham kinase 3e-26 [PIRKW] phosphotransferase 3e-26 [PIRKW] nucleus le-06 [PIRKW] plasma 9e-08 [PIRKW] duplication 3e-25 [PIRKW] hormone 9e-08 [PIRKW] zinc 3e-09 [PIRKW] cell cycle control 4e-13 [PIRKW] transmembrane protein 3e-12 [PIRKW] zinc finger le-08 [PIRKW] stomach 9e-08 [PIRKW] DNA binding 9e-06 [PIRKW] autophosphorylation 3e-26 [PIRKW] phosphoprotem 3e-26 [PIRKW] signal transduction 5e-08 [PIRKW] heterotrimer 5e-08 [PIRKW] coiled coil 3e-26 [PIRKW] multimer 3e-26 [PIRKW] transcription regulation 4e-10 [PIRKW] GTP binding 5e-08 [SUPFAM] chromobox homology 9e-06 [SUPFAM] RING finger homology 3e-09 [SUPFAM] coatomer complex beta' chain le-07 [SUPFAM] WD repeat homology 3e-26 [SUPFAM] yeast coatomer complex alpha chain 3e-12 [SUPFAM] GTP-bindmg regulatory protein beta chain 5e-08 [SUPFAM] PRL1 protein 2e-09 [PROSITE] WD_REPEA S 2 [PROSITE] LEUCINE_ZIPPER 1 [PROSITE] MYRISTYL 14 [PROSITE] CK2_PHOSPHO_SITE 4 [PROSITE] ZINC_FINGER_C3HC4 1 [PROSITE] PKC_PHOSPHO_SITE 18 [PROSITE] ASN_GLYCOSYLATION 1 [PFAM] Zmc finger, C3HC4 type (RING finger) [PFAM] WD domain, G-beta repeats [KW] Irregular [KW] 3D [KW] LOW_COMPLEXITY 6.23 % [KW] COILED COIL 6.73 % SEQ MPPISTPRRSDSAISVRSLHSESSMSLRSTFSLPEEEEEPEPLVFAEQPSVKLCCQLCCS SEG xxxxxxxxxxxxxx .... xxxxxxxxx COILS lgg2B SEQ VFKDPVITTCGHTFCRRCALKSEKCPVDNVKLTVVVNNIAVAEQIGELFIHCRHGCRVAG SEG COILS lgg2B SEQ SGKPPIFEVDPRGCPFTIKLSARKDHEGSCDYRPVRCPNNPSCPPLLRMNLEAHLKECEH SEG COILS lgg2B SEQ IKCPHSKYGCTFIGNQDTYETHLETCRFEGLKEFLQQTDDRFHEMHVALAQKDQEIAFLR SEG COILS cccccccccccccc lgg2B SEQ SMLGKLSEKIDQLEKSLELKFDVLDENQSKLSEDLMEFRRDASMLNDELSHINARLNMGI SEG COILS cccccccccccccccccccccccccc lgg2B SEQ LGSYDPQQIFKCKGTFVGHQGPVWCLCVYSMGDLLFSGSSDKTIKVWDTCTTYKCQKTLE SEG COILS lgg2B . EECCCCCCEEEEEETTTTCEEEEEETTTEEEEEEG-GGCEEEEEEE SEQ GHDGIVLALCIQGCKLYSGSADCTIIVWDIQNLQKVNTIRAHDNPVCTLVSSHNVLFSGS SEG COILS lgg2B CCCCCEEEEEETTCEEEEEETTTCEEEEETTTTEEEEEE-CTTTTCCCEEE. SEQ LKAIKVWDIVGTELKLKKELTGLNHWVRALVAAQSYLYSGSYQTIKIWDIRTLDCIHVLQ SEG xxxxxxxxxxxxx COILS lgg2B SEQ TSGGSVYSIAVTNHHIVCGTYENLIHVWDIESKEQVRTLTGHVGTVYALAVISTPDQTKV SEG COILS lgg2B SEQ FSASYDRSLRVWSMDNMICTQTLLRHQGSVTALAVSRGRLFSGAVDSTVKVWTC SEG COILS lgg2B Prosite for DKFZphutel_lι2.2 PS00001 267->271 ASN_GLYCOSYLATION PDOC00001 PS00005 6->9 PKC_PHOSPHO_SITE PDOC00005 PS00005 15->18 PKC_PHOSPHO_SITE PDOC00005 PS00005 26->29 PKC_PHOSPHO_SITE PDOC00005 PS00005 50->53 PKC_PHOSPHO_SITE PDOC00005 PS00005 82->85 PKC_PHOSPHO_SITE PDOC00005 PS00005 121->124 PKC_PHOSPHO_SITE PDOC00005 PS00005 137->140 PKC_PHOΞPHO_SITE PDOC00005 PS00005 141->144 PKC_PHOSPHO_SITE PDOC00005 PS00005 205->208 PKC_PHOSPHO_SITE PDOC00005 PS00005 247->250 PKC_PHOSPHO_SITE PDOC00005 PS00005 340->343 PKC_PHOSPHO_SITE PDOC00005 PS00005 343->346 PKC_PHOSPHO_SITE PDOC00005 PS00005 352->355 PKC_PHOSPHO_SITE PDOC00005 PS00005 398->401 PKC_PHOSPHO_SITE PDOC00005 PS00005 420->423 PKC_PHOSPHO_SITE PDOC00005 PS00005 464->467 PKC_PHOSPHO_SITE PDOC00005 PS00005 548->551 PKC_PHOSPHO_SITE PDOC00005 PS00005 588->591 PKC_PHOSPHO_SITE PDOC00005 PS00006 32->36 CK2_PHOSPHO_SITE PDOC00006 PS00006 201->205 CK2_PHOSPHO_SITE PDOC00006 PS00006 330->334 CK2_PHOSPHO_SITE PDOC00006 PS00006 533->537 CK2_PHOSPHO_SITE PDOC00006 PS00008 115->121 MYRISTYL PDOC00008 PS00008 133->139 MYRISTYL PDOC00008 PS00008 194->200 MYRISTYL PDOC00008 PS00008 299->305 MYRISTYL PDOC00008 PS00008 314->320 MYRISTYL PDOC00008 PS00008 364->370 MYRISTYL PDOC00008 PS00008 379->385 MYRISTYL PDOC00008 PS00008 419->425 MYRISTYL PDOC00008 PS00008 460->466 MYRISTYL PDOC00008 PS00008 484->490 MYRISTYL PDOC00008 PS00008 499->505 MYRISTYL PDOC00008 PS00008 524->530 MYRISTYL PDOC00008 PS00008 568->574 MYRISTYL PDOC00008 PS00008 583->589 MYRISTYL PDOC00008 PS00518 70->80 ZINC_FINGER_C3HC4 PDOC00449 PS00029 436->458 LEUCINE_ZIPPER PDOC00029 PS00678 335->350 WD_REPEATS PDOC00574 PS00678 376->391 WD REPEATS PDOC00574 Pfam for DKFZphutel_lι2.2 HMM_NAME WD domain, G-beta repeats HMM *MrGHnnWVWCVaFSPDGrWFIvSGSWDgTCRLWD* ++GH ++VWC+ + G + ++SGS D+T+++WD Query 316 FVGHQGPVWCLCVYSMGDL-LFSGSSDKTIKVWD 348 22.93 519 553 1 34 dkfzphutel_lι2.2 similarity to Dictostelium myosin heavy chain kinase Alignment to HMM consensus: Query *MrGHnnWVWCVaF.. SPDGrWFIvSGSWDgTCRLWD* ++GH ++V+++A+ +PD ++S+S D+++R+W+ dkfzphutel 519 LTGHVGTVYALAVISTPDQTK-VFSASYDRSLRVWS 553 HMM_NAME Zinc fmger, C3HC4 type (RING finger) HMM *CPICFcTFQlDyPWPFdePmMlPCgHsFCypCIrrW..CPmC* C++C + F++P++++CGH+FC+ C +++ CP+ Query 55 CQLC CSV FKDPVITTCGHTFCRRCALKSEKCPVD DKFZphutel 20bl 9 group : metabolism DKFZphute l_20bl 9 encodes a novel 486 amino acid protein with similarity to bacterial sarcosine oxidases ( EC 1 . 5 . 3 . 1 . ) The novel protein seems to be a novel enzyme with sarcosine oxidase activity . The new protein can find application in modulation of sarcosine metabolism and as a new enzyme for biotechnologic production processes . similarity to sarcosine oxidases membrane regions : 1 Summary DKFZphutel_20bl 9 encodes a novel 486 amino acid protein, with similarity to sarcosine oxidases . similarity to sarcosine oxidases complete cDNA'' , complete eds potential start at Bp 48 , EST hits , Sequenced by AGOWA Locus : unknown Insert length : 1967 bp Poly A stretch at pos . 1950 , no polyadenylation signal found 1 AGCGAGGCAG CAGTGCAGCT TTCAGAGGGT CCGGGCTCAG AGGGGTTATG 51 ATTCGGAGGG TTCTGCCGCA CGGCATGGGC CGGGGCCTCT TGACCCGGAG 101 GCCAGGCACG CGCAGAGGAG GCTTTTCTCT GGACTGGGAT GGAAAGGTGT 151 CTGAGATTAA GAAGAAGATC AAGTCGATCC TGCCTGGAAG GTCCTGTGAT 201 CTACTGCAAG ACACCAGCCA CCTGCCTCCC GAGCACTCGG ATGTGGTGAT 251 CGTGGGAGGT GGGGTGCTTG GCTTGTCTGT GGCCTATTGG CTGAAGAAGC 301 TGGAGAGCAG ACGAGGTGCT ATTCGAGTGC TAGTGGTGGA ACGGGACCAC 351 ACGTATTCAC AGGCCTCCAC TGGGCTCTCA GTAGGTGGGA TTTGTCAGCA 401 GTTCTCATTG CCTGAGAACA TCCAGCTCTC CCTCTTTTCA GCCAGCTTTC 451 TACGGAACAT CAATGAGTAC CTGGCCGTAG TCGATGCTCC TCCCCTGGAC 501 CTCCGGTTCA ACCCCTCGGG CTACCTCTTG CTGGCTTCAG AAAAGGATGC 551 TGCAGCCATG GAGAGCAACG TGAAAGTGCA GAGGCAGGAG GGAGCCAAAG 601 TTTCTCTGAT GTCTCCTGAT CAGCTTCGGA ACAAGTTTCC CTGGATAAAC 651 ACAGAGGGAG TGGCTTTGGC GTCTTATGGG ATGGAGGACG AAGGTTGGTT 701 TGACCCCTGG TGTCTGCTCC AGGGGCTTCG GCGAAAGGTC CAGTCCTTGG 751 GAGTCCTTTT CTGCCAGGGA GAGGTGACAC GTTTTGTCTC TTCATCTCAA 801 CGCATGTTGA CCACAGATGA CAAAGCGGTG GTCTTGAAAA GGATCCATGA 851 AGTCCATGTG AAGATGGACC GCAGCCTGGA GTACCAGCCT GTGGAATGCG 901 CCATTGTGAT CAACGCAGCC GGAGCCTGGT CTGCGCAAAT CGCAGCACTG 951 GCTGGTGTTG GAGAGGGGCC GCCTGGCACC CTGCAGGGCA CCAAGCTACC 1001 TGTGGAGCCG AGGAAAAGGT ATGTGTATGT GTGGCACTGC CCCCAGGGAC 1051 CAGGCCTAGA GACTCCGCTT GTTGCAGACA CCAGTGGAGC CTATTTTCGC 1101 CGGGAAGGAT TAGGTAGCAA CTACCTAGGT GGTCGTAGCC CCACTGAGCA 1151 GGAAGAACCG GACCCGGCGA ACCTGGAAGT GGACCATGAT TTCTTCCAGG 1201 ACAAGGTGTG GCCCCATTTG GCCCTGAGGG TCCCAGCTTT TGAGACTCTG 1251 AAGGTTCAGA GCGCCTGGGC CGGCTATTAC GACTACAACA CCTTTGACCA 1301 GAATGGCGTG GTGGGCCCCC ACCCGCTAGT TGTCAACATG TACTTTGCTA 1351 CTGGCTTCAG TGGTCACGGG CTCCAGCAGG CCCCTGGCAT TGGGCGAGCT 1401 GTAGCAGAGA TGGTACTGAA GGGCAGGTTC CAGACCATCG ACCTGAGCCC 1451 CTTCCTCTTT ACCCGCTTTT ACTTGGGAGA GAAGATCCAG GAGAACAACA 1501 TCATCTGAGC ATGTGTGCTC TGCACTGGCT CCACTGGCTT GCATCCTGGC 1551 TGTGTTCACA GCCTTGTTTG CTGCTTCCAT CTTCCCCAGT ACTGTGCCAG 1601 GCCTTCTCCC CCTCCCCAGT GTCCTCTCCT CTCAGGCAGG CCATTGCACC 1651 CATATGGCTG GGCAGGCACA GGCAGTGAGG CCGAGGCCAA TAGCGAGTGA 1701 TGAGCGGGAT CCTAGGACTG ATCTGTAGCC CATGCTGATG TCACCCACCA 1751 GGGCAATCCA TCTGGAGGCC TGAGCACCCT GGCCCAGGAC TGGCTTCATC 1801 CTGGCACTGA CCAGGAAAGA CTGCCTCTGA CCCTCTTAGC AGACAGAGCC 1851 CAGGCATGGG AGCACTCTGG GGCAGCCTGG CTCAGGTTTA TTGATTTTCG 1901 TCTGTTTACC CTATCCATTA ATCAATACAT GTAATTAACT CCTTCCCTCC 1951 AAAAAAAAAA AAAAAAA BLAST Results o BLAST result Medline entries No Medline entry Peptide information for frame 3 ORF from 48 bp to 1505 bp; peptide length: 486 Category: similarity to known protein 1 MIRRVLPHGM GRGLLTRRPG TRRGGFSLDW DGKVSEIKKK IKSILPGRSC 51 DLLQDTSHLP PEHSDVVIVG GGVLGLSVAY WLKKLESRRG AIRVLVVERD 101 HTYSQASTGL SVGGICQQFS LPENIQLSLF SASFLRNINE YLAVVDAPPL 151 DLRFNPSGYL LLASEKDAAA MESNVKVQRQ EGAKVSLMΞP DQLRNKFPWI 201 NTEGVALASY GMEDEGWFDP WCLLQGLRRK VQSLGVLFCQ GEVTRFVSSS 251 QRMLTTDDKA VVLKRIHEVH VKMDRSLEYQ PVECAIVINA AGAWSAQIAA 301 LAGVGEGPPG TLQGTKLPVE PRKRYVYVWH CPQGPGLETP LVADTSGAYF 351 RREGLGSNYL GGRSPTEQEE PDPANLEVDH DFFQDKVWPH LALRVPAFET 401 LKVQSAWAGY YDYNTFDQNG VVGPHPLVVN MYFATGFSGH GLQQAPGIGR 451 AVAEMVLKGR FQTIDLSPFL FTRFYLGEKI QENNII BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphutel_20bl9, frame 3 TREMBL :CEM04B2_4 gene: "M04B2.4"; Caenorhabditis elegans cosmid M04B2, N = 1, Score = 801, P = 9.2e-80 PIR:B71184 probable sarcosine oxidase - Pyrococcus horikoshn, N = 2, Score = 194, P = 2e-26 PIR:B69284 sarcosine oxidase, subunit beta (soxB) homolog - Archaeoglobus fulgidus, N = 3, Score = 189, P = 8.2e-22 TREMBL: AF042732_1 gene: "Bb"; product: "unknown protein"; Anopheles gambiae (Bb) gene, partial eds; and TU37B2 (TU37B2) and diphenol oxιdase-A2 (Dox-A2) genes, complete eds., N = 1, Score = 386, P = 8.7e-36 PIR:F71008 probable sarcosine oxidase - Pyrococcus horikoshn, N = 2, Score = 200, P = 4e-25 >TREMBL:CEM04B2_4 gene: "M04B2.4"; Caenorhabditis elegans cosmid M04B2 Length = 527 HSPs: Score = 801 (120.2 bits), Expect = 9.2e-80, P = 9.2e-80 Identities = 171/433 (39%), Positives = 260/433 (60%) Query: 61 PEHSDVVIVGGGVLGLSVAYWLKKLESRRGAIRVLVVERDHTYSQASTGLSVGGICQQFS 120 P +++VI+GGG+ G S A+WLK+ R +V+VVE + ++++ST LS GGI QQFS Sbjct: 91 PYRAEIVIIGGGLSGSSTAFWLKE-RFRDEDFKVVVVENNDVFTKSSTMLSTGGITQQFS 149 Query: 121 LPENIQLSLFSASFLRNINEYLAVVDAPPLDLRFNPSGYLLLA-SEKDAAAMESNVKVQR 179 +PE + +SLF+ FLR+ E+L ++D+ D+ F P+GYL LA ++++ M S KVQ Sbjct: 150 IPEFVDMSLFTTEFLRHAGEHLRILDSEQPDINFFPTGYLRLAKTDEEVEMMRSAWKVQI 209 Query: 180 QEGAKVSLMSPDQLRNKFPWINTEGVALASYGMEDEGWFDPWCLLQGLRRKVQSLGVLFC 239 + GAKV L+S D+L ++P++N + V LAS G+E+EG D W LL +R K +LGV + Sbjct: 210 ERGAKVQLLSKDELTKRYPYMNVDDVLLASLGVENEGTIDTWQLLSAIREKNITLGVQYV 269 Query: 240 QGEVTRFVSSSQRM LTTDDKAVVLKRIHEVHVKMDRS-LEYQPVECAIVI 288 +GEV F R T D+ + +RI V V+ + +P+ +++ Sbjct: 270 KGEVEGFQFERHRASSEVHAFGDDATADENKLRAQRISGVLVRPQMNDASARPIRAHLIV 329 Query: 289 NAAGAWSAQIAALAGVGEGPPGTLQGTKLPVEPRKRYVYVWHCPQGPGLETPLVADTS-G 347 NAAG W+ Q+A +AG+G+G G L +P++PRKR V+V P P + P + D S G Sbjct: 330 NAAGPWAGQVAKMAGIGKGT-GLL-AVPVPIQPRKRDVFVIFAPDVPS-DLPFIIDPSTG 386 Query: 348 AYFRREGLGSNYLGGRSPTEQEEP—DPANLEVDHDFFQDKVWPHLALRVPAFETLKVQS 405 + R+ G +L GR+P+++E+ D +NL+VD+D F K+WP L RVP F+T KV+S Sbjct: 387 VFCRQTDSGQTFLVGRTPSKEEDAKRDHSNLDVDYDDFYQKIWPVLVDRVPGFQTAKVKS 446 Query: 406 AWAGYYDYNTFDQNGVVGPHPLVVNMYFATGFSGHGLQQAPGIGRAVAEMVLKGRFQTID 465 AW+GY D NTFD V+G HPL N++ GF G+ + RA AE + G + ++ Sbjct: 447 AWSGYQDINTFDDAPVIGEHPLYTNLHMMCGFGERGVMHSMAAARAYAERIFDGAYINVN 506 Query: 466 LSPFLFTRFYLGEKIQE 482 L F R + I E Sbjct: 507 LRKFDMRRIVKMDPITE 523 Pedant information for DKFZphutel_20bl9, frame 3 Report for DKFZphutel_20bl9.3 [LENGTH] 486 [MW] 53811.85 [pi] 7.66 [HOMOL] TREMBL :CEM04B2_4 gene: "M04B2.4' Caenorhabditis elegans cosmid M04B2 le-78 [FUNCAT] c energy conversion [H. influenzae, HI0499] 8e-05 [BLOCKS] BL00677A D-amino acid oxidases proteins [BLOCKS] BL00623A GMC oxidoreductases proteins [BLOCKS] BL01304A [EC] 1.5.99.2 Dimethylglycine dehydrogenase 2e-07 [PIRKW] flavoprotem 2e-07 [PIRKW] oxidoreductase 2e-07 [PROSITE] MYRISTYL 12 [PROSITE] CK2_PHOSPHO_SITE 5 [PROSITE] GLYCOSAMINOGLYCAN 1 [PROSITE] PKC_PHOSPHO_SITE 6 [KW] TRANSMEMBRANE 1 [KW] LOW COMPLEXITY 7.00 % SEQ MIRRVLPHGMGRGLLTRRPGTRRGGFSLDWDGKVSEIKKKIKSILPGRSCDLLQDTSHLP SEG xxxxxxxxxxxxxxx xxxxxxx PRD ccceeecccccceeecccccccccccccccccchhhhhhhhhhccccccceeeccccccc MEM SEQ PEHΞDVVIVGGGVLGLSVAYWLKKLESRRGAIRVLVVERDHTYSQASTGLSVGGICQQFS SEG xxxxxxxxxxx PRD cccceeeeeccccchhhhhhhhhhhhhhcccceeeeeeccccccccccccccccceeeec MEM MMMMMMMMMMMMMMMMM SEQ LPENIQLSLFSASFLRNINEYLAVVDAPPLDLRFNPSGYLLLASEKDAAAMESNVKVQRQ SEG PRD ccchhhhhhhhhhhhhhhhhhhhhhhccccceeecccceeeehhhhhhhhhhhhhhhhhh MEM SEQ EGAKVSLMSPDQLRNKFPWINTEGVALASYGMEDEGWFDPWCLLQGLRRKVQSLGVLFCQ SEG PRD cccceeecccchhhhhhccccccccccccccccccccccccchhhhhhhhhhhheeeeec MEM SEQ GEVTRFVSSSQRMLTTDDKAVVLKRIHEVHVKMDRSLEYQPVECAIVINAAGAWSAQIAA SEG PRD ceeeeecccccccccccchhhhhhhhhheeeecccccccccceeeeeeecccchhhhhhh MEM SEQ LAGVGEGPPGTLQGTKLPVEPRKRYVYVWHCPQGPGLETPLVADTSGAYFRREGLGSNYL SEG PRD hhccccccccccccccccccccceeeeeeecccccccccceeeccccceeeeccccccee MEM SEQ GGRSPTEQEEPDPANLEVDHDFFQDKVWPHLALRVPAFETLKVQSAWAGYYDYNTFDQNG SEG PRD ecccccccccccccccccccchhhhhhhhhhhhhhcchhhhhhhhhhheeeeeccccccc MEM SEQ VVGPHPLVVNMYFATGFSGHGLQQAPGIGRAVAEMVLKGRFQTIDLSPFLFTRFYLGEKI SEG PRD cccccccccceeeecccccccccchhhhhhhhhhhhhhccceeeeccccccccccccccc MEM SEQ QENNII SEG PRD CCCCCC MEM OOOOOOOOOOOOOOOOOOOOOOOO OOOOOOOOOOOOOOOOOOOOOOOO OOOOOOOOOOOOOOOOOOOOOOOO OOOOOOOOOOOOOOOOOOOOOOOO DKFZphutel_20g21 group: signal transduction DKFZphutel_20g21 encodes a novel 861 amino ac d protein with partial similarity to human ras inhibitor and other ras inhibitor proteins. Ras is a signal transducting molecule involved m the receptor tyrosine kinase/RAS/Map kinase signalling cascade. Ras proteins bind GDP/GTP and show intrinsic GTPase activity. Mutations in ras, which change aa 12, 13 or 61 activate the potential of ras to transform cultured cells and are implicated in a variety of human tumours. The novel protein seems to be a new ras inhibitor protein. The new protein can find application m modulating/blocking ras dependent signal transduction pathways . Ras inhibitor additional 1188 Bp at 5' and 1107 at 3' end in comparison to 122483 Sequenced by AGOWA Locus : unknown Insert length: 4137 bp Poly A stretch at pos. 4116, no polyadenylation signal found 1 GGGAGAACTG AAACAGGAGA TGGTGCGGAC AGATGTCAAC CTGGAAAATG 51 GCCTGGAACC CGCTGAAACC CACAGCATGG TAAGACACAA GGATGGTGGC 101 TATTCCGAGG AAGAGGACGT GAAGACCTGT GCCCGGGACT CAGGCTATGA 151 CAGCCTCTCC AACAGGCTCA GCATCTTGGA CCGGCTCCTC CACACCCACC 201 CCATATGGCT GCAGCTGAGT CTGAGTGAGG AGGAGGCAGC AGAGGTCCTG 251 CAGGCCCAGC CTCCGGGGAT CTTCCTGGTT CATAAATCTA CCAAGATGCA 301 GAAGAAAGTC CTCTCCCTCC GCCTGCCCTG TGAATTTGGG GCCCCACTCA 351 AGGAATTTGC CATAAAGGAA AGCACATACA CCTTTTCCCT GGAAGGCTCA 401 GGAATCAGTT TCGCAGATTT ATTCCGGCTC ATTGCTTTCT ACTGCATCAG 451 CAGGGATGTT CTACCATTTA CCTTGAAGTT GCCTTATGCC ATTTCAACAG 501 CCAAGTCGGA GGCTCAGCTT GAAGAACTGG CCCAGATGGG ACTAAATTTC 551 TGGAGCTCCC CAGCTGACAG CAAACCCCCG AACCTTCCAC CTCCCCATAG 601 GCCTCTTTCC TCCGACGGTG TCTGTCCTGC CTCCCTGCGT CAGCTCTGCC 651 TTATAAATGG AGTGCATTCT ATCAAAACCA GGACGCCTTC AGAGCTGGAG 701 TGCAGCCAGA CCAACGGGGC CCTGTGCTTT ATTAATCCCC TTTTCTTGAA 751 AGTGCACAGC CAGGACCTCA GTGGAGGCCT GAAACGGCCG AGCACAAGGA 801 CTCCCAACGC GAATGGCACG GAGCGGACTC GGTCCCCCCC ACCCAGGCCC 851 CCGCCACCCG CTATTAATAG TCTCCACACA AGCCCTCGGC TGGCCAGGAC 901 TGAAACCCAG ACGAGCATGC CAGAAACAGT CAACCATAAC AAACATGGGA 951 ACGTAGCTCT GCCTGGAACG AAACCAACTC CCATCCCTCC ACCCCGGCTG 1001 AAGAAGCAGG CTTCTTTTCT GGAAGCAGAG GGCGGTGCAA AGACCTTGAG 1051 CGGCGGCCGG CCGGGCGCAG GCCCGGAGCT GGAGCTGGGC ACAGCTGGCA 1101 GCCCAGGTGG GGCCCCGCCT GAGGCCGCCC CGGGGGATTG CACAAGGGCC 1151 CCGCCGCCCA GCTCTGAATC ACGGCCCCCG TGCCATGGAG GCCGGCAGCG 1201 GCTGAGCGAC ATGAGCATTT CTACTTCCTC CTCCGACTCG CTGGAGTTCG 1251 ACCGGAGCAT GCCTCTGTTT GGCTACGAGG CGGACACCAA CAGCAGCCTG 1301 GAGGACTACG AGGGGGAAAG TGACCAAGAG ACCATGGCGC CCCCCATCAA 1351 GTCCAAAAAG AAAAGGAGCA GCTCCTTCGT GCTGCCCAAG CTCGTCAAGT 1401 CCCAGCTGCA GAAGGTGAGC GGGGTGTTCA GCTCCTTCAT GACCCCGGAG 1451 AAGCGGATGG TCCGCAGGAT CGCCGAGCTT TCCCGGGACA AATGCACCTA 1501 CTTCGGGTGC TTAGTGCAGG ACTACGTGAG CTTCCTGCAG GAGAACAAGG 1551 AGTGCCACGT GTCCAGCACC GACATGCTGC AGACCATCCG GCAGTTCATG 1601 ACCCAGGTCA AGAACTATTT GTCTCAGAGC TCGGAGCTGG ACCCCCCCAT 1651 CGAGTCGCTG ATCCCTGAAG ACCAAATAGA TGTGGTGCTG GAAAAAGCCA 1701 TGCACAAGTG CATCTTGAAG CCCCTCAAGG GGCATGTGGA GGCCATGCTG 1751 AAGGACTTTC ACATGGCCGA TGGCTCATGG AAGCAACTCA AGGAGAACCT 1801 GCAGCTTGTG CGGCAGAGGA ATCCGCAGGA GCTGGGGGTC TTCGCCCCGA 1851 CCCCTGATTT TGTGGATGTG GAGAAAATCA AAGTCAAGTT CATGACCATG 1901 CAGAAGATGT ATTCGCCGGA AAAGAAGGTC ATGCTGCTGC TGCGGGTCTG 1951 CAAGCTCATT TACACGGTCA TGGAGAACAA CTCAGGGAGG ATGTATGGCG 2001 CTGATGACTT CTTGCCAGTC CTGACCTATG TCATAGCCCA GTGTGACATG 2051 CTTGAATTGG ACACTGAAAT CGAGTACATG ATGGAGCTCC TAGACCCATC 2101 GCTGTTACAT GGAGAAGGAG GCTATTACTT GACAAGCGCA TATGGAGCAC 2151 TTTCTCTGAT AAAGAATTTC CAAGAAGAAC AAGCAGCGCG ACTGCTCAGC 2201 TCAGAAACCA GAGACACCCT GAGGCAGTGG CACAAACGGA GAACCACCAA 2251 CCGGACCATC CCCTCTGTGG ACGACTTCCA GAATTACCTC CGAGTTGCAT 2301 TTCAGGAGGT CAACAGTGGT TGCACAGGAA AGACCCTCCT TGTGAGACCT 2351 TACATCACCA CTGAGGATGT GTGTCAGATC TGCGCTGAGA AGTTCAAGGT 2401 GGGGGACCCT GAGGAGTACA GCCTCTTTCT CTTCGTTGAC GAGACATGGC 2451 AGCAGCTGGC AGAGGACACT TACCCTCAAA AAATCAAGGC GGAGCTGCAC 2501 AGCCGACCAC AGCCCCACAT CTTCCACTTT GTCTACAAAC GCATCAAGAA 2551 CGATCCTTAT GGCATCATTT TCCAGAACGG GGAAGAAGAC CTCACCACCT 2601 CCTAGAAGAC AGGCGGGACT TCCCAGTGGT GCATCCAAAG GGGAGCTGGA 2651 AGCCTTGCCT TCCCGCTTCT ACATGCTTGA GCTTGAAAAG CAGTCACCTC 2701 CTCGGGGACC CCTCAGTGTA GTGACTAAGC CATCCACAGG CCAACTCGGC 2751 CAAGGGCAAC TTTAGCCACG CAAGGTAGCT GAGGTTTGTG AAACAGTAGG 2801 ATTCTCTTTT GGCAATGGAG AATTGCATCT GATGGTTCAA GTGTCCTGAG 2851 ATTGTTTGCT ACCTACCCCC AGTCAGGTTC TAGGTTGGCT TACAGGTATG 2901 TATATGTGCA GAAGAAACAC TTAAGATACA AGTTCTTTTG AATTCAACAG 2951 CAGATGCTTG CGATGCAGTG CGTCAGGTGA TTCTCACTCC TGTGGATGGC 3001 TTCATCCCTG CCTTCCTTCC TTTCTTTTTC CTTTTTTTTT TTTTTTTTTT 3051 TTTTTACAAA GAGCCTTCAT GTTTTTATAT ATTTCATAGA AATTTTTATA 3101 GCAGTTGCAG GTAAACTGTC AGGATTGGTT TTAAAATATT TTTGTAACTT 3151 TAAAATATTC TATAATTATG CATGTGATTT TAACATTTAA TATTCAAAAA 3201 TAAATCTCTT GCTGGATTTG AGAGTATTGC ATTTTTAAAG TCTCTCTTCT 3251 GTAACTGGAT GTTTTGGCAA CTTTGTGGGG AGAGACTGCT GGATTTCTTA 3301 AAGCAACGTA TTCCTGACAC TGGCCACAGA ATGCCTTTGG AAATCGGATG 3351 TACTGTTCTC TTGTTCACGT TTAGTGGTGT TTTGCTGTTT TGTTTTTTAA 3401 ACAAATGATG CTGAGAATAA GGAGAGAAAT GAATGTAGAG AGAGGTAGAG 3451 AGAGAAATAT GAACTCTAAC AAAGGACTGA GGAGTGCAGT CTGCTGGTTC 3501 AGGCTCTTCA AAAGATGTAG AAAAAGAGAT AGAAGGAACC ACCTATGCTT 3551 AAAATACTGT AAATATGCAG TGAGGTTTGG CAAAATCTAT TCCATGTGTG 3601 ATTTGCTTGT AGAAACAATT TTGAAAGCCC CTTGAGGAAA ATAAAAATCA 3651 AGAAGAACAC TTTTCTCCCT TTTCCATACA AATTAAAACT TAACAGCATC 3701 AAATTATTGG GACCAGAAAC CAAGTAATGT ATAATGTGGC TTTTGTTGAG 3751 TTAAATAAGA TGCTATATAA TGGAGAAGAA TTTGAAAATG CACAAAAAAA 3801 TCAATCTACA TTATCAGAAC CTGCAGTGAA ATTAAACTTA TGTTAAATAA 3851 AACCAGTTTG CAGGTGCACA AACTATGAGG GTCTTGTATC CACGTAACAC 3901 AGGTAGT AC AAAAACATGT TATTGTACTG TGTAAAGATG CATAGTCATC 3951 TCATTTGGTT GGCTTTGTAC CTTGTACCTT TTTTAGCCTT GGCTTTTGTT 4001 GAACTAGAAC CCTCAGCACA TACTGTGTTG TACTTTTGTA AATGATTTTT 4051 TAAATGGAAT TTTGCACATA ATACATTGTA ATACTGTATG ATAATCATGT 4101 GTGAAAATAA TTTTTGAAAT AAAAAAAAAA AAAAAAA BLAST Results Entry 122483 from database EMBL: Sequence 15 from patent US 5527896. Length = 1829 Plus Strand HSPs: Score = 9097 (1364.9 bits), Expect = 0.0, P = 0.0 Identities = 1821/1823 (99%), Positives = 1821/1823 (99%) Medlme entries No Medline entry Peptide information for frame 2 ORF from 20 bp to 2602 bp; peptide length: 861 Category: known protein Classification: Cell signaling/communication 1 MVRTDVNLEN GLEPAETHSM VRHKDGGYSE EEDVKTCARD SGYDSLSNRL 51 SILDRLLHTH PIWLQLSLSE EEAAEVLQAQ PPGIFLVHKS TKMQKKVLSL 101 RLPCEFGAPL KEFAIKESTY TFSLEGSGIS FADLFRLIAF YCISRDVLPF 151 TLKLPYAIST AKSEAQLEEL AQMGLNFWSS PADSKPPNLP PPHRPLSΞDG 201 VCPASLRQLC LINGVHSIKT RTPSELECSQ TNGALCFINP LFLKVHSQDL 251 SGGLKRPSTR TPNANGTERT RSPPPRPPPP AINSLHTSPR LARTETQTSM 301 PETVNHNKHG NVALPGTKPT PIPPPRLKKQ ASFLEAEGGA KTLSGGRPGA 351 GPELELGTAG SPGGAPPEAA PGDCTRAPPP SSESRPPCHG GRQRLSDMSI 401 STSSSDΞLEF DRSMPLFGYE ADTNSSLEDY EGESDQETMA PPIKSKKKRS 451 SSFVLPKLVK SQLQKVSGVF SSFMTPEKRM VRRIAELSRD KCTYFGCLVQ 501 DYVSFLQENK ECHVSSTDML QTIRQFMTQV KNYLSQSSEL DPPIESLIPE 551 DQIDVVLEKA MHKCILKPLK GHVEAMLKDF HMADGSWKQL KENLQLVRQR 601 NPQELGVFAP TPDFVDVEKI KVKFMTMQKM YSPEKKVMLL LRVCKLIYTV 651 MENNSGRMYG ADDFLPVLTY VIAQCDMLEL DTEIEYMMEL LDPSLLHGEG 701 GYYLTSAYGA LSLIKNFQEE QAARLLSSET RDTLRQWHKR RTTNRTIPSV 751 DDFQNYLRVA FQEVNSGCTG KTLLVRPYIT TEDVCQICAE KFKVGDPEEY 801 SLFLFVDETW QQLAEDTYPQ KIKAELHSRP QPHIFHFVYK RIKNDPYGII 851 FQNGEEDLTT S BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphutel_20g21, frame 2 TREMBL: RNU80076_1 product: "RIN1"; Rattus norvegicus RIN1 mRNA, complete eds., N = 3, Score = 606, P = 6.8e-97 PIR:A38637 Ras interactor RIN1 - human, N = 3, Score = 587, P = 1.9e-92 TREMBL :HSRASINL_1 product: "ras inhibitor"; Human ras inhibitor mRNA, 3' end., N = 2, Score = 592, P = 9.8e-61 SWISSPROT -RIN1_HUMAN RAS INTERACTION/INTERFERENCE PROTEIN 1 (RAS INHIBITOR JC99) (FRAGMENT)., N = 2, Score = 587, P = 4.1e-60 PIR:B38637 Ras inhibitor (clone JC265) - human (fragment), N = 1, Score = 2446, P = 4.6e-254 >PIR:B38637 Ras inhibitor (clone JC265) human (fragment) Length = 471 HSPs: Score = 2446 (367.0 bits), Expect = 4.6e-254, P = 4.6e-254 Identities = 471/471 (100%), Positives = 471/471 (100%) Query: 391 GRQRLSDMSISTSSSDSLEFDRSMPLFGYEADTNSSLEDYEGESDQETMAPPIKSKKKRS 450 GRQRLSDMSISTSSSDSLEFDRSMPLFGYEADTNSSLEDYEGESDQETMAPPIKSKKKRS Sbjct: 1 GRQRLSDMSISTΞSSDSLEFDRSMPLFGYEADTNSSLEDYEGESDQETMAPPIKSKKKRS 60 Query: 451 SΞFVLPKLVKSQLQKVSGVFSSFMTPEKRMVRRIAELSRDKCTYFGCLVQDYVSFLQENK 510 SSFVLPKLVKSQLQKVSGVFSSFMTPEKRMVRRIAELSRDKCTYFGCLVQDYVSFLQENK Sbjct: 61 ΞSFVLPKLVKSQLQKVSGVFSSFMTPEKRMVRRIAELSRDKCTYFGCLVQDYVSFLQENK 120 Query: 511 ECHVSSTDMLQTIRQFMTQVKNYLSQSSELDPPIESLIPEDQIDVVLEKAMHKCILKPLK 570 ECHVSSTDMLQTIRQFMTQVKNYLSQSSELDPPIESLIPEDQIDVVLEKAMHKCILKPLK Sbjct: 121 ECHVSSTDMLQTIRQFMTQVKNYLSQSSELDPPIESLIPEDQIDVVLEKAMHKCILKPLK 180 Query: 571 GHVEAMLKDFHMADGΞWKQLKENLQLVRQRNPQELGVFAPTPDFVDVEKIKVKFMTMQKM 630 GHVEAMLKDFHMADGSWKQLKENLQLVRQRNPQELGVFAPTPDFVDVEKIKVKFMTMQKM Sbjct: 181 GHVEAMLKDFHMADGSWKQLKENLQLVRQRNPQELGVFAPTPDFVDVEKIKVKFMTMQKM 240 Query: 631 YSPEKKVMLLLRVCKLIYTVMENNSGRMYGADDFLPVLTYVIAQCDMLELDTEIEYMMEL 690 YSPEKKVMLLLRVCKLIYTVMENNSGRMYGADDFLPVLTYVIAQCDMLELDTEIEYMMEL Sbjct: 241 YSPEKKVMLLLRVCKLIYTVMENNSGRMYGADDFLPVLTYVIAQCDMLELDTEIEYMMEL 300 Query: 691 LDPSLLHGEGGYYLTSAYGALSLIKNFQEEQAARLLSSETRDTLRQWHKRRTTNRTIPSV 750 LDPSLLHGEGGYYLTSAYGALSLIKNFQEEQAARLLSSETRDTLRQWHKRRTTNRTIPSV Sbjct: 301 LDPSLLHGEGGYYLTSAYGALSLIKNFQEEQAARLLSSETRDTLRQWHKRRTTNRTIPSV 360 Query: 751 DDFQNYLRVAFQEVNSGCTGKTLLVRPYITTEDVCQICAEKFKVGDPEEYSLFLFVDETW 810 DDFQNYLRVAFQEVNSGCTGKTLLVRPYITTEDVCQICAEKFKVGDPEEYSLFLFVDETW Sbjct: 361 DDFQNYLRVAFQEVNSGCTGKTLLVRPYITTEDVCQICAEKFKVGDPEEYSLFLFVDETW 420 Query: 811 QQLAEDTYPQKIKAELHSRPQPHIFHFVYKRIKNDPYGIIFQNGEEDLTTS 861 QQLAEDTYPQKIKAELHΞRPQPHIFHFVYKRIKNDPYGIIFQNGEEDLTTS Sbjct: 421 QQLAEDTYPQKIKAELHSRPQPHIFHFVYKRIKNDPYGIIFQNGEEDLTTS 471 Pedant information for DKFZphutel_20g21, frame 2 Report for DKFZphutel_20g21.2 [LENGTH] 861 [MW] 96380.26 [pi] 6.15 [HOMOL] PIR:B38637 Ras inhibitor (clone JC265) - human (fragment) 0.0 [FUNCAT] 08.13 vacuolar transport [S. cerevisiae, YML097c] 3e-10 [ FUNCAT ] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YML097c] 3e-10 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YML097c] 3e-10 [FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YML097c] 3e-10 [PIRKW] alternative splicing 3e-59 [SUPFAM] Ras interactor RIN1 3e-59 [KW] All_Alpha [KW] LOW_COMPLEXITY 11.27 % SEQ MVRTDVNLENGLEPAETHSMVRHKDGGYSEEEDVKTCARDSGYDSLΞNRLSILDRLLHTH SEG PRD ccccceeeccccccccceeeeeecccccccccceeeeeeccccccchhhhhhhhhhhhhh SEQ PIWLQLSLSEEEAAEVLQAQPPGIFLVHKSTKMQKKVLSLRLPCEFGAPLKEFAIKESTY SEG ... xxxxxxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhhccccceeeeechhhhhhhhhhhcccccccccceeeeeeecc SEQ TFSLEGSGISFADLFRLIAFYCISRDVLPFTLKLPYAISTAKSEAQLEELAQMGLNFWSS SEG PRD ceeecccccchhhhhhhhhhhhhcceeeeeecccchhhhhhhhhhhhhhhhhhccccccc SEQ PADSKPPNLPPPHRPLSSDGVCPASLRQLCLINGVHSIKTRTPSELECSQTNGALCFINP SEG xxxxxxxxxx PRD cccccccccccccccccccccccchhhhhhcccccccccccccccccccccccceeeecc SEQ LFLKVHSQDLSGGLKRPΞTRTPNANGTERTRSPPPRPPPPAINSLHTSPRLARTETQTSM SEG xxxxxxxx PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ PETVNHNKHGNVALPGTKPTPIPPPRLKKQASFLEAEGGAKTLSGGRPGAGPELELGTAG SEG xxxxxxxxxxx xx PRD eeeeeccccccccccccccccccccchhhhhhhhhhhccccccccccccccceeeeeccc SEQ SPGGAPPEAAPGDCTRAPPPΞSESRPPCHGGRQRLΞDMSISTSSSDSLEFDRSMPLFGYE SEG xxxxxxxxxxxx xxxxxxxxxx xxxxxxxxxxxxxxxxxx PRD ccccccccccccccccccccccccccccccccccccccccccccccceeeccccccceee SEQ ADTNSSLEDYEGESDQETMAPPIKSKKKRSSSFVLPKLVKSQLQKVSGVFSSFMTPEKRM SEG xxxxxxxxx PRD cccccccccccccccccccccccccccccccccccccchhhhhhhhhhhhhhhhcchhhh SEQ VRRIAELSRDKCTYFGCLVQDYVSFLQENKECHVSSTDMLQTIRQFMTQVKNYLSQSSEL SEG PRD hhhhhhhhhhchhhhhhhhhhhhhhhhcccccccccchhhhhhhhhhhhhhhhhhhhhcc SEQ DPPIEΞLIPEDQIDVVLEKAMHKCILKPLKGHVEAMLKDFHMADGSWKQLKENLQLVRQR SEG PRD ccccccccccchhhhhhhhhhhhhccccchhhhhhhhhhhhhccccchhhhhhhhhhhhh SEQ NPQELGVFAPTPDFVDVEKIKVKFMTMQKMYSPEKKVMLLLRVCKLIYTVMENNSGRMYG SEG PRD ccccccccccccccchhhhhhhhhhhhhhhccccchhhhhhhhhhhhhhhhhhhcccccc SEQ ADDFLPVLTYVIAQCDMLELDTEIEYMMELLDPSLLHGEGGYYLTSAYGALSLIKNFQEE SEG PRD cccccccceeecccccchhhhhhhhhhhhhhcccccccccceeeeehhhhhhhhhhhhhh SEQ QAARLLSSETRDTLRQWHKRRTTNRTIPSVDDFQNYLRVAFQEVNSGCTGKTLLVRPYIT SEG PRD hhhhhhhhhhhhhhhhhhhhhhhcccccccchhhhhhhhhhhccccccceeeeecccccc SEQ TEDVCQICAEKFKVGDPEEYSLFLFVDETWQQLAEDTYPQKIKAELHSRPQPHIFHFVYK SEG PRD chhhhhhhhhheeecccccceeeeehhhhhhcccccccchhhhhhhhhccccceeeehhh SEQ RIKNDPYGIIFQNGEEDLTTS SEG PRD hhccccceeeeeccccccccc (No Prosite data available for DKFZphutel_20g21.2) (No Pfam data available for DKFZphutel_20g21.2) DKFZphutel 20hl3 group: intracellular transport and trafficking DKFZphutel_20hl3 encodes a novel 955 ammo acid protein with similarity to alpha-adaptins . Adaptms are components of the adaptor complexes which link clathrm to receptors in coated vesicles. The alpha-adaptins, which are found exclusively in endocytic coated vesicles, separate into two bands on SDS gels, designated A and C. The novel protein is very similar to both alpha adaptin A and C. The novel protein is a new human alpha-adaptin. The new protein can find application in modulating endocytosis and vesicle trafficking in cells . strong similarity to alpha-adaptins complete cDNA, complete eds start at Bp 78, EST hits Sequenced by AGOWA Locus: unknown Insert length: 3352 bp Poly A stretch at pos. 3297, polyadenylation signal at pos. 3279 1 GCGCCCGGTC CCCGCTTGCC AGCCCCCGCT GCTCTGTGCC CTGTCCGGCC 51 AGGCCTGGAG CCGACACCAC CGCCATCATG CCGGCCGTGT CCAAGGGCGA 101 TGGGATGCGG GGGCTCGCGG TGTTCATCTC CGACATCCGG AACTGTAAGA 151 GCAAAGAGGC GGAAATTAAG AGAATCAACA AGGAACTGGC CAACATCCGC 201 TCCAAGTTCA AAGGAGACAA AGCCTTGGAT GGCTACAGTA AGAAAAAATA 251 TGTGTGTAAA CTGCTTTTCA TCTTCCTGCT TGGCCATGAC ATTGACTTTG 301 GGCACATGGA GGCTGTGAAT CTGTTGAGTT CCAATAAATA CACAGAGAAG 351 CAAATAGGTT ACCTGTTCAT TTCTGTGCTG GTGAACTCGA ACTCGGAGCT 401 GATCCGCCTC ATCAACAACG CCATCAAGAA TGACCTGGCC AGCCGCAACC 451 CCACCTTCAT GTGCCTGGCC CTGCACTGCA TCGCCAACGT GGGCAGCCGG 501 GAGATGGGCG AGGCCTTTGC CGCTGACATC CCCCGCATCC TGGTGGCCGG 551 GGACAGCATG GACAGTGTCA AGCAGAGTGC GGCCCTGTGC CTCCTTCGAC 601 TGTACAAGGC CTCGCCTGAC CTGGTGCCCA TGGGCGAGTG GACGGCGCGT 651 GTGGTACACC TGCTCAATGA CCAGCACATG GGTGTGGTCA CGGCCGCCGT 701 CAGCCTCATC ACCTGTCTCT GCAAGAAGAA CCCAGATGAC TTCAAGACGT 751 GCGTCTCTCT GGCTGTGTCG CGCCTGAGCC GGATCGTCTC CTCTGCCTCC 801 ACCGACCTCC AGGACTACAC CTACTACTTC GTCCCAGCAC CCTGGCTCTC 851 GGTGAAGCTC CTGCGGCTGC TGCAGTGCTA CCCGCCTCCA GAGGATGCGG 901 CTGTGAAGGG GCGGCTGGTG GAATGTCTGG AGACTGTGCT CAACAAGGCC 951 CAGGAGCCCC CCAAATCCAA GAAGGTGCAG CATTCCAACG CCAAGAACGC 1001 CATCCTCTTC GAGACCATCA GCCTCATCAT CCACTATGAC AGTGAGCCCA 1051 ACCTCCTGGT TCGGGCCTGC AACCAGCTGG GCCAGTTCCT GCAGCACCGG 1101 GAGACCAACC TGCGCTACCT GGCCCTGGAG AGCATGTGCA CGCTGGCCAG 1151 CTCCGAGTTC TCCCATGAAG CCGTCAAGAC GCACATTGAC ACCGTCATCA 1201 ATGCCCTCAA GACGGAGCGG GACGTCAGCG TGCGGCAGCG GGCGGCTGAC 1251 CTCCTCTACG CCATGTGTGA CCGGAGCAAT GCCAAGCAGA TCGTGTCGGA 1301 GATGCTGCGG TACCTGGAGA CGGCAGACTA CGCCATCCGC GAGGAGATCG 1351 TCCTGAAGGT GGCCATCCTG GCCGAGAAGT ACGCCGTGGA CTACAGCTGG 1401 TACGTGGACA CCATCCTCAA CCTCATCCGC ATTGCGGGCG ACTACGTGAG 1451 TGAGGAGGTG TGGTACCGTG TGCTACAGAT CGTCACCAAC CGTGATGACG 1501 TCCAGGGCTA TGCCGCCAAG ACCGTCTTTG AGGCGCTCCA GGCCCCTGCC 1551 TGTCACGAGA ACATGGTGAA GGTTGGCGGC TACATCCTTG GGGAGTTTGG 1601 GAACCTGATT GCTGGGGACC CCCGCTCCAG CCCCCCAGTG CAGTTCTCCC 1651 TGCTCCACTC CAAGTTCCAT CTGTGCAGCG TGGCCACGCG GGCGCTGCTG 1701 CTGTCCACCT ACATCAAGTT CATCAACCTC TTCCCCGAGA CCAAGGCCAC 1751 CATCCAGGGC GTCCTGCGGG CCGGCTCCCA GCTGCGCAAT GCTGACGTGG 1801 AGCTGCAGCA GCGAGCCGTG GAGTACCTCA CCCTCAGCTC AGTGGCCAGC 1851 ACCGACGTCC TGGCCACGGT GCTGGAGGAG ATGCCGCCCT TCCCCGAGCG 1901 CGAGTCGTCC ATCCTGGCCA AGCTGAAACG CAAGAAGGGG CCAGGGGCCG 1951 GCAGCGCCCT GGACGATGGC CGGAGGGACC CCAGCAGCAA CGACATCAAC 2001 GGGGGCATGG AGCCCACCCC CAGCACTGTG TCGACGCCCT CGCCCTCCGC 2051 CGACCTCCTG GGGCTGCGGG CAGCCCCTCC CCCGGCAGCA CCCCCGGCTT 2101 CTGCAGGAGC AGGGAACCTT CTGGTGGACG TCTTCGATGG CCCGGCCGCC 2151 CAGCCCAGCC TGGGGCCCAC CCCCGAGGAG GCCTTCCTCA GCCCAGGTCC 2201 TGAGGACATC GGCCCTCCCA TTCCGGAAGC CGATGAGTTG CTGAATAAGT 2251 TTGTGTGTAA GAACAACGGG GTCCTGTTCG AGAACCAGCT GCTGCAGATC 2301 GGAGTCAAGT CAGAGTTCCG ACAGAACCTG GGCCGCATGT ATCTCTTCTA 2351 TGGCAACAAG ACCTCGGTGC AGTTCCAGAA TTTCTCACCC ACTGTGGTTC 2401 ACCCGGGAGA CCTCCAGACT CAGCTGGCTG TGCAGACCAA GCGCGTGGCG 2451 GCGCAGGTGG ACGGCGGCGC GCAGGTGCAG CAGGTGCTCA ATATCGAGTG 2501 CCTGCGGGAC TTCCTGACGC CCCCGCTGCT GTCCGTGCGC TTCCGGTACG 2551 GTGGCGCCCC CCAGGCCCTC ACCCTGAAGC TCCCAGTGAC CATCAACAAG 2601 TTCTTCCAGC CCACCGAGAT GGCGGCCCAG GATTTCTTCC AGCGCTGGAA 2651 GCAGCTGAGC CTCCCTCAAC AGGAGGCGCA GAAAATCTTC AAAGCCAACC 2701 ACCCCATGGA CGCAGAAGTT ACTAAGGCCA AGCTTCTGGG GTTTGGCTCT 2751 GCTCTCCTGG ACAATGTGGA CCCCAACCCT GAGAACTTCG TGGGGGCGGG 2801 GATCATCCAG ACTAAAGCCC TGCAGGTGGG CTGTCTGCTT CGGCTGGAGC 2851 CCAATGCCCA GGCCCAGATG TACCGGCTGA CCCTGCGCAC CAGCAAGGAG 2901 CCCGTCTCCC GTCACCTGTG TGAGCTGCTG GCACAGCAGT TCTGAGCCCT 2951 GGACTCTGCC CCGGGGGATG TGGCCGGCAC TGGGCAGCCC CTTGGACTGA 3001 GGCAGTTTTG GTGGATGGGG GACCTCCACT GGTGACAGAG AAGACACCAG 3051 GGTTTGGGGG ATGCCTGGGA CTTTCCTCCG GCCTTTTGTA TTTTTATTTT 3101 TGTTCATCTG CTGCTGTTTA CATTCTGGGG GGTTAGGGGG AGTCCCCCTC 3151 CCTCCCTTTC CCCCCCAAGC ACAGAGGGGA GAGGGGCCAG GGAAGTGGAT 3201 GTCTCCTCCC CTCCCACCCC ACCCTGTTGT AGCCCCTCCT ACCCCCTCCC 3251 CATCCAGGGG CTGTGTATTA TTGTGAGCGA ATAAACAGAG AGACGCTAAA 3301 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 3351 AA BLAST Results No BLAST result Medl e entries 89155572: Cloning of cDNAs encoding two related 100-kD coated vesicle proteins (alpha-adaptins) . 97431776: Alpha-adaptm, a marker for endocytosis, is expressed m complex patterns during Drosophila development . Peptide information for frame 3 ORF from 78 bp to 2942 bp; peptide length: 955 Category: strong similarity to known protein 1 MPAVSKGDGM RGLAVFISDI RNCKSKEAEI KRINKELANI RSKFKGDKAL 51 DGYSKKKYVC KLLFIFLLGH DIDFGHMEAV NLLSSNKYTE KQIGYLFISV 101 LVNSNSELIR LINNAIKNDL ASRNPTFMCL ALHCIANVGS REMGEAFAAD 151 IPRILVAGDS MDSVKQSAAL CLLRLYKASP DLVPMGEWTA RVVHLLNDQH 201 MGVVTAAVSL ITCLCKKNPD DFKTCVSLAV SRLSRIVSSA STDLQDYTYY 251 FVPAPWLSVK LLRLLQCYPP PEDAAVKGRL VECLETVLNK AQEPPKSKKV 301 QHSNAKNAIL FETISLIIHY DSEPNLLVRA CNQLGQFLQH RETNLRYLAL 351 ESMCTLAΞSE FSHEAVKTHI DTVINALKTE RDVSVRQRAA DLLYAMCDRS 401 NAKQIVSEML RYLETADYAI REEIVLKVAI LAEKYAVDYS WYVDTILNLI 451 RIAGDYVSEE VWYRVLQIVT NRDDVQGYAA KTVFEALQAP ACHENMVKVG 501 GYILGEFGNL IAGDPRSSPP VQFSLLHSKF HLCSVATRAL LLSTYIKFIN 551 LFPETKATIQ GVLRAGSQLR NADVELQQRA VEYLTLSSVA STDVLATVLE 601 EMPPFPERES SILAKLKRKK GPGAGSALDD GRRDPSSNDI NGGMEPTPST 651 VSTPSPSADL LGLRAAPPPA APPASAGAGN LLVDVFDGPA AQPSLGPTPE 701 EAFLSPGPED IGPPIPEADE LLNKFVCKNN GVLFENQLLQ IGVKSEFRQN 751 LGRMYLFYGN KTSVQFQNFS PTVVHPGDLQ TQLAVQTKRV AAQVDGGAQV 801 QQVLNIECLR DFLTPPLLSV RFRYGGAPQA LTLKLPVTIN KFFQPTEMAA 851 QDFFQRWKQL SLPQQEAQKI FKANHPMDAE VTKAKLLGFG SALLDNVDPN 901 PENFVGAGII QTKALQVGCL LRLEPNAQAQ MYRLTLRTSK EPVSRHLCEL 951 LAQQF BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphutel_20hl3, frame 3 PIR:B30111 alpha-adaptm C - mouse, N = 1, Score = 3990, P = 0 PIR:S11276 alpha-adaptm c - rat, N = 1, Score = 3987, P = 0 SWISSPROT :ADAC_RAT ALPHA-ADAPTIN C (CLATHRIN ASSEMBLY PROTEIN COMPLEX 2 ALPHA-C LARGE CHAIN) (100 KD COATED VESICLE PROTEIN C) (PLASMA MEMBRANE ADAPTOR HA2/AP2 ADAPTIN ALPHA C SUBUNIT)., N = 1, Score = 3982, P = 0 SWISSPROT :ADAC_MOUSE ALPHA-ADAPTIN C (CLATHRIN ASSEMBLY PROTEIN COMPLEX 2 ALPHA-C LARGE CHAIN) (100 KD COATED VESICLE PROTEIN C) (PLASMA MEMBRANE ADAPTOR HA2/AP2 ADAPTIN ALPHA C SUBUNIT)., N = 1, Score = 3976, P = 0 TREMBL :AB020706_1 gene: "KIAA0899"; product: "KIAA0899 protein"; Homo sapiens mRNA for KIAA0899 protein, partial eds., N = 1, Score = 3932, P = 0 >PIR:B30111 alpha-adaptm C - mouse Length = 938 HSPs: Score = 3990 (598.6 bits), Expect = O.Oe+00, P = O.Oe+00 Identities = 787/955 (82%), Positives = 858/955 (89%) Query: 1 MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVC 60 MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVC Sbjct: 1 MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVC 60 Query: 61 KLLFIFLLGHDIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDL 120 KLLFIFLLGHDIDFGHMEAVNLLSSN+YTEKQIGYLFIΞVLVNSNSELIRLINNAIKNDL Sbjct: 61 KLLFIFLLGHDIDFGHMEAVNLLSSNRYTEKQIGYLFISVLVNSNSELIRLINNAIKNDL 120 Query: 121 ASRNPTFMCLALHCIANVGSREMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASP 180 ASRNPTFM LALHCIANVGSREM EAFA +IP+ILVAGD+MDSVKQSAALCLLRLY+ SP Sbjct: 121 ASRNPTFMGLALHCIANVGSREMAEAFAGEIPKILVAGDTMDΞVKQSAALCLLRLYRTSP 180 Query: 181 DLVPMGEWTARVVHLLNDQHMGVVTAAVSLITCLCKKNPDDFKTCVSLAVSRLSRIVSSA 240 DLVPMG+WT+RVVHLLNDQH+GVVTAA SLIT L +KNP++FKT VSLAVSRLSRIV+SA Sbjct: 181 DLVPMGDWTSRVVHLLNDQHLGVVTAATSLITTLAQKNPEEFKTSVSLAVSRLSRIVTSA 240 Query: 241 STDLQDYTYYFVPAPWLΞVKLLRLLQCYPPPEDAAVKGRLVECLETVLNKAQEPPKSKKV 300 STDLQDYTYYFVPAPWLSVKLLRLLQCYPPP D AV+GRL ECLET+LNKAQEPPKSKKV Sbjct: 241 STDLQDYTYYFVPAPWLSVKLLRLLQCYPPP-DPAVRGRLTECLETILNKAQEPPKSKKV 299 Query: 301 QHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSE 360 QHSNAKNA+LFE ISLIIH+DSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSE Sbjct: 300 QHSNAKNAVLFEAISLIIHHDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSE 359 Query: 361 FSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAI 420 FSHEAVKTHI+TVINALKTERDVSVRQRA DLLYAMCDRSNA+QIV+EML YLETADY+I Sbjct: 360 FSHEAVKTHIETVINALKTERDVSVRQRAVDLLYAMCDRSNAQQIVAEMLSYLETADYSI 419 Query: 421 REEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAA 480 REEIVLKVAILAEKYAVDY+WYVDTILNLIRIAGDYVSEEVWYRV+QIV NRDDVQGYAA Sbjct: 420 REEIVLKVAILAEKYAVDYTWYVDTILNLIRIAGDYVSEEVWYRVIQIVINRDDVQGYAA 479 Query: 481 KTVFEALQAPACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRAL 540 KTVFEALQAPACHEN+VKVGGYILGEFGNLIAGDPRSSP +QF+LLHSKFHLCSV TRAL Sbjct: 480 KTVFEALQAPACHENLVKVGGYILGEFGNLIAGDPRSSPLIQFNLLHSKFHLCSVPTRAL 539 Query: 541 LLSTYIKFINLFPETKATIQGVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLE 600 LLSTYIKF+NLFPE KATIQ VLR+ SQL+NADVELQQRAVEYL LS+VASTD+LATVLE Sbjct: 540 LLSTYIKFVNLFPEVKATIQDVLRSDSQLKNADVELQQRAVEYLRLSTVASTDILATVLE 599 Query: 601 EMPPFPERESSILAKLKRKKGPGAGSALDDGRRDPSSNDINGGMEPTP STVSTPSPS 657 EMPPFPERESSILAKLK+KKGP + L++ +R+ S D+NGG EP P S STPSPS Sbjct: 600 EMPPFPERESSILAKLKKKKGPSTVTDLEETKRERSI-DVNGGPEPVPASTSAASTPSPS 658 Query: 658 ADLLGLRAAPP-PAAPPASAGAGNLLVDVFDGPAAQPSLGPTPEEAFLSPGPEDIGPPIP 716 ADLLGL A PP P PP S+G G LLVDVF A+ ++ P L+PG ED Sbjct: 659 ADLLGLGAVPPAPTGPPPSSGGG-LLVDVFSDSAS--AVAP LAPGSEDN 704 Query: 717 EADELLNKFVCKNNGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQNFSPTVVHP 776 +FVCKNNGVLFENQLLQIG+KSEFRQNLGRM++FYGNKTS QF NF+PT++ Sbjct: 705 FARFVCKNNGVLFENQLLQIGLKSEFRQNLGRMFIFYGNKTSTQFLNFTPTLICA 759 Query: 777 GDLQTQLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGAPQALTLKLP 836 DLQT L +QTK V VDGGAQVQQV+NIEC+ DF P+L+++FRYGG Q +++KLP Sbjct: 760 DDLQTNLNLQTKPVDPTVDGGAQVQQVVNIECISDFTEAPVLNIQFRYGGTFQNVSVKLP 819 Query: 837 VTINKFFQPTEMAAQDFFQRWKQLSLPQQEAQKIFKANHPMDAEVTKAKLLGFGSALLDN 896 +T+NKFFQPTEMA+QDFFQRWKQLS PQQE Q IFKA HPMD E+TKAK++GFGSALL+ Sbjct: 820 ITLNKFFQPTEMASQDFFQRWKQLSNPQQEVQNIFKAKHPMDTEITKAKIIGFGSALLEE 879 Query: 897 VDPNPENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF 955 VDPNP NFVGAGII TK Q+GCLLRLEPN QAQMYRLTLRTSK+ VS+ LCELL++QF Sbjct: 880 VDPNPANFVGAGIIHTKTTQIGCLLRLEPNLQAQMYRLTLRTSKDTVSQRLCELLSEQF 938 Pedant information for DKFZphutel_20hl3, frame 3 Report for DKFZphutel_20hl3.3 [LENGTH] 955 [MW] 105361.97 [pi] 7.75 [HOMOL] PIR:A30111 alpha-adaptin A - mouse 0.0 [FUNCAT] 30.09 organization of intracellular transport vesicles [S. cerevisiae, YBL037w] 5e-67 [FUNCAT] 08.19 cellular import [S. cerevisiae, YBL037w] 5e-67 [FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YBL037w] 5e-67 [FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YDR238c] 4e-04 [PIRKW] heterodimer 0.0 [PIRKW] transmembrane protein le-65 [PIRKW] membrane trafficking 0.0 [PIRKW] receptor 0.0 [SUPFAM] beta-adaptin 5e-16 [PROSITE] MYRISTYL 7 [PROSITE] IG_MHC 1 [PROSITE] AMIDATION 1 [PROSITE] CK2_PHOSPHO_SITE 11 [PROSITE] TYR_PHOSPHO_SITE 3 [PROSITE] PKC_PHOSPHO_SITE 15 [PROSITE] ASN_GLYCOSYLATION 1 [KW] All_Alpha [KW] LOW_COMPLEXITY 6.81 % SEQ MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVC SEG PRD ccccccccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccccchhhhhhh SEQ KLLFIFLLGHDIDFGHMEAVNLLSΞNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDL SEG PRD hhhhhhhcccccccchhhhhhhhhcccccchhhhhhhhhhhhhcchhhhhhhhhhhhhcc SEQ ASRNPTFMCLALHCIANVGSREMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASP SEG PRD cccccchhhhhhhhhhccchhhhhhhhhhhhhheeeccccchhhhhhhhhhhhhhhhhcc SEQ DLVPMGEWTARVVHLLNDQHMGVVTAAVSLITCLCKKNPDDFKTCVSLAVSRLSRIVSSA SEG PRD cccccccchhhhhhhhhcccceeeehhhhhhhhhhhcccchhhhhhhhhhhhhhhhhhcc SEQ STDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAVKGRLVECLETVLNKAQEPPKSKKV SEG PRD ccccccceeeecccchhhhhhhhhhhhhcccccchhhhhhhhhhhhhhhhhhhccccccc SEQ QHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSE SEG PRD cccccchhhhhhhhhhhhhcccccceeeeehhhhhhhhhhccccceeeehhhhhhhhhcc SEQ FSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAI SEG PRD cchhhhhhhhhhhhhhhccccchhhhhhhhhhhhhhhcccchhhhhhhhhhhhhhcccch SEQ REEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAA SEG PRD hhhhhhhhhhhhhhhhccchhhhhhhhhhhhhhccccchhhhhhhheeeccccchhhhhh SEQ KTVFEALQAPACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRAL SEG PRD hhhhhhhhhhcccccceeeeeeeecccccccccccccccchhhhhhhhhhhcccchhhhh SEQ LLSTYIKFINLFPETKATIQGVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLE SEG PRD hhhhhhhhhhccccchhhhhhhhhhhcccchhhhhhhhhhhhhhhhhccchhhhhhhhhh SEQ EMPPFPERESSILAKLKRKKGPGAGSALDDGRRDPSSNDINGGMEPTPSTVSTPSPSADL SEG xxxxxxxxxxxxxxx PRD hccccccchhhhhhhhhhccccccccccccccccccccccccccccccccccccccccce SEQ LGLRAAPPPAAPPASAGAGNLLVDVFDGPAAQPSLGPTPEEAFLΞPGPEDIGPPIPEADE SEG xxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxx. PRD eecccccccccccccccccceeeeeeccccccccccccccceeecccccccccccccccc SEQ LLNKFVCKNNGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQNFSPTVVHPGDLQ SEG PRD cceeeeeccccccchhhhhhhhcchhhhhccccceeeccccccccccccceeeeccchhh SEQ TQLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGAPQALTLKLPVTIN SEG xxxxxxxxxxxxxx PRD hhhhhhhhcccccccccchhhhhhhhhhccccccccceeeeeeccccccccccccccccc SEQ KFFQPTEMAAQDFFQRWKQLSLPQQEAQKIFKANHPMDAEVTKAKLLGFGSALLDNVDPN SEG PRD cccccchhhhhhhhhhhhhhhchhhhhhhhhhhcccchhhhhhhhhhccccceeeecccc SEQ PENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF SEG PRD ccceeeceeeeeccccceeeeecccchhhhhhhhhhhccccchhhhhhhhhhccc Prosite for DKFZphutel_20hl3.3 PS00001 760->764 ASN_GLYCOSYLATION PDOC00001 PS00005 54->57 PKC_PHOSPHO_SITE PDOC00005 PS00005 85->88 PKC_PHOSPHO_SITE PDOC00005 PS00005 89->92 PKC_PHOSPHO_SITE PDOC00005 PS00005 163->166 PKC_PHOSPHO_SITE PDOC00005 PS00005 189->192 PKC_PHOSPHO_SITE PDOC00005 PS00005 258->261 PKC_PHOSPHO_SITE PDOC00005 PS00005 297->300 PKC_PHOSPHO_SITE PDOC00005 PS00005 379->382 PKC_PHOSPHO_SITE PDOC00005 PS00005 384->387 PKC_PHOSPHO_SITE PDOC00005 PS00005 470->473 PKC_PHOSPHO_SITE PDOC00005 PS00005 787->790 PKC_PHOSPHO_SITE PDOC00005 PS00005 819->822 PKC_PHOSPHO_SITE PDOC00005 PS00005 832->835 PKC_PHOSPHO_SITE PDOC00005 PS00005 935->938 PKC_PHOSPHO_SITE PDOC00005 PS00005 938->941 PKC_PHOSPHO_SITE PDOC00005 PS00006 5->9 CK2_PHOSPHO_SITE PDOC00006 PS00006 104->108 CK2_PHOSPHO_SITE PDOC00006 PS00006 368->372 CK2_PHOSPHO_SITE PDOC00006 PS00006 379->383 CK2_PHOSPHO_SITE PDOC00006 PS00006 470->474 CK2_PHOSPHO_SITE PDOC00006 PS00006 482->486 CK2_PHOSPHO_SITE PDOC00006 PS00006 597->601 CK2_PHOSPHO_SITE PDOC00006 PS00006 626->630 CK2_PHOSPHO_SITE PDOC00006 PS00006 636->640 CK2_PHOSPHO_SITE PDOC00006 PS00006 698->702 CK2_PHOSPHO_SITE PDOC00006 PS00006 938->942 CK2_PHOSPHO_SITE PDOC00006 PS00007 388->395 TYR_PHOSPHO_SITE PDOC00007 PS00007 411->419 TYR_PHOSPHO_SITE PDOC00007 PS00007 434->443 TYR_PHOSPHO_SITE PDOC00007 PS00008 202->208 MYRISTYL PDOC00008 PS00008 508->514 MYRISTYL PDOC00008 PS00008 561->567 MYRISTYL PDOC00008 PS00008 623->629 MYRISTYL PDOC00008 PS00008 759->765 MYRISTYL PDOC00008 PS00008 826->832 MYRISTYL PDOC00008 PS00008 908->914 MYRISTYL PDOC00008 PS00009 630->634 AMIDATION PDOC00009 PS00290 127->134 IG MHC PDOC00262 (No Pfam data available for DKFZphutel_20hl3.3) DKFZphutel_20mll group: cell cycle DKFZphutel_20mll encodes a novel 225 amino acid protein with similarity to yeast sds22 and protein phosphatase-1 regulatory subunits. sds22 is a regulatory polypeptide of protein phosphatase-1 that is required for the completion of mitosis in both fission and budding yeast. The novel protein seems to be a new regulator protein for protein phosphatase-1. The new protein can find application in modulating/blocking the activity of protein phosphatase-1 and in modulating the cell cycle. similarity to suppressor protein sds22 complete cDNA, complete eds, EST hits localisation? only a part of the STS matches Sequenced by AGOWA Locus: /map="17"? Insert length: 5822 bp Poly A stretch at pos. 5803, polyadenylation signal at pos. 5786 1 GGGCGCTTGG TTCCCCAGCA ACCGGGAGAC GCGTCTGCTG CGTGGAACCG 51 CCGAGTTCCC AGCGCTTGAG AAGGAAAATT CTGGATCTGT TATCTGTGAG 101 GAGGCCACTC CGTTGACAGT TGTGTAAAAC TCTGCTGCTT TCCCCAGCTC 151 CAACCTCTCT GGTCTTCAAC AACACTATCA TCAGGGAAAA CGTGGGGGAA 201 GATGAACCAG CCGTGCAACT CGATGGAGCC GAGGGTGATG GACGATGACA 251 TGCTCAAGCT GGCCGTCGGG GACCAGGGCC CCCAGGAGGA GGCCGGGCAG 301 CTGGCCAAGC AGGAGGGCAT CCTCTTCAAG GATGTCCTGT CCCTGCAGCT 351 GGACTTTCGG AACATCCTCC GCATAGACAA CCTCTGGCAG TTTGAGAACT 401 TGAGGAAGCT GCAGCTGGAC AATAACATCA TTGAGAAGAT CGAGGGCCTG 451 GAGAACCTCG CACACCTGGT CTGGCTGGAT CTGTCTTTCA ACAACATTGA 501 GACCATCGAG GGGCTGGACA CACTGGTGAA CCTGGAGGAC CTGAGCTTGT 551 TCAACAACCG GATCTCCAAG ATCGACTCCC TGGACGCCCT CGTCAAGCTG 601 CAGGTGTTGT CGCTGGGCAA CAACCGGATT GACAACATGA TGAACATCAT 651 CTACCTCCGG CGGTTCAAGT GCCTGCGGAC GCTCAGCCTC TCTAGGAACC 701 CTATCTCTGA GGCAGAGGAT TACAAGATGT TCATCTGTGC CTACCTTCCT 751 GACCTCATGT ACCTGGACTA CCGGCGCATT GATGACCACA CAGCAAGTGT 801 CTCCCTCTCA GTCTCCCAGC CCTGTGAGAC AGATTCCTCA AGCCCCCAGG 851 TTTCTTGGAA AAGGGGCATT GAAGAGTAGC TTCCCCTGCC CACAACTAGG 901 AGAGAAAGGG CAGCTCCCTC TTCCTAATCC CTTTACCTGA CTCTGTCAGA 951 GTGATTCCAG CAGCACCCTT GTAAGTACTG TTTTGTGTGC GTTCCCAGGG 1001 GCCAGGCCTC TTCCACACAC TGTCCCAGGG CCACCTCACA GCCATCCTGC 1051 ACTGTCTAGT TTTCCAGATG AAGAAGCTGA GGAGGGCTGG GAGCAGTGGC 1101 TCACGCCTGT AATCCCAGCA CTTTGAGAGG CTGAGGCGGG AGGATCGCTT 1151 GAGCCAAGGA GTTCAAGACC AGCCTGGGCA ACATAGGGAG ACCCCATCTC 1201 TACAGAAACT ACCAAAATTA GCCAGGTGTG GTGGCACACA CCAGTAATCC 1251 TGGCTACTCA CAAGGCCGAG GTAGAAGAAT CGCTTGAGAC TAGGAGTTTG 1301 AGGCTGCAGT GAACTAAGAA GATGCCATTG CACTCCAGCC TGGGCAACAG 1351 AGTGAAAAAA TTAAAAAATT AGAAAAGAAA AGAAGTTGAG GAGGCCCAAG 1401 GAGGGCAAGC AGCCAGGATC ACTGGCTCAA GGCCAAGCCA GGATTCACCC 1451 TAAGTTGGTG TCATCCCAGG AGCAATATTA ACAGCTGAGC TCCAGAGGGA 1501 ACCAGGCCAT CAGAGGCTCA GGCCTGGCTC TCAGGGGCAG AGTCAGGGCT 1551 GGAGGTAGAG ACCTGAGTGT CATCTGAGGA TTGCCAATTG GCAGTAGTTG 1601 AAGCCATGGT ACAGGTGGGA TCACCTGGGG CACATGGAGT GAGCTGGGGG 1651 ACGGGGACTA AGTTCTAGAG GTGCCAGCAT TCCTGGCCAG GTACAGGGGG 1701 ATGAGCCAGT GCGGTGGAGA GAGCCAAGGG CCAGACCCTC GTGACCAGCC 1751 CTATGGCCTC ACTCTACCTC TGTCCTGTTG TCCTCCTTCC CTAAAAGAGG 1801 GCCAGAAGGC CTGCTGAGGG CTGTTGGGAG TGAGAGAGCA AGTCCTCTGT 1851 GGAGAACACC CAGTCTGGGG CGAGGGGAGC GCTCCATTGC TGTGGCTCCT 1901 GCCCTGGAGA TGGCCCCGGG AACCCCAGCC TGCCACGCTG CCTTCCGCTC 1951 CTCCTGGTCT TTCCCTGATT TCCCTGCGCT CACAAAAACC TGGTGAGGGT 2001 CATCAGGAGA TGGGCATTCT CATCCACGAG ACCTCATGGC TTTCACAGCC 2051 TTCATGCAGG CCCCTGTGCA ACACCCCTGC CCATGCGCGG GAGGCTGCAG 2101 CATGGCAGAG GCGGCATGGC AGAGGCGGTG TGGCTCGGAG GAACCTCTGG 2151 TAACAATGCC ACTCCCGTTC CCTGGTCAGA AAAAGCTTGC GGAGGCTAAG 2201 CACCAGTACA GCATCGACGA GCTGAAGCAC CAGGAGAACC TGATGCAGGC 2251 CCAGCTGGAG GACGAGCAGG CGCAGCGGGA GGAGCTAGAG AAGCACAAGA 2301 CTGCGTTTGT GGAACACCTG AATGGCTCCT TCCTGTTTGA CAGCATGTAC 2351 GCTGAGGACT CAGAGGGCAA CAATCTGTCC TACCTGCCTG GTGTCGGTGA 2401 GCTCCTTGAG ACCTACAAGG ACAAGTTTGT CATCATCTGC GTGAATATTT 2451 TTGAGTATGG CCTGAAACAG CAGGAGAAGC GGAAAACAGA GCTTGACACC 2501 TTCAGTGAAT GTGTCCGTGA GGCCATCCAG GAAAACCAGG AGCAGGGCAA 2551 ACGCAAGATT GCCAAATTCG AGGAGAAGCA CTTGTCGAGT TTAAGTGCCA 2601 TTCGAGAGGA GTTGGAACTG CCCAACATTG AGAAGATGAT CCTAGAATGC 2651 AGTGCTGACA TCAGTGAGTT GTTCGATGCG CTCATGACGC TGGAGATGCA 2701 GCTGGTGGAG CAGCTGGAGG TAAGGCTGGG CCCTGGGCAC AAGTGCCAGA 2751 ATCTGGCGAT GCAGCTGCAC ATCCATAGGT GAACTGTAGC CTTCATGGGC 2801 ACGCCTCTGC TGGAAACGTC CAGCACGACT CAGCGTGGCA GGCTGTAGCT 2851 TTCTTGCTCA TCAGTCCTGT TTGCTTTTAT TACATTTTAA TCATTTACAT 2901 TGGAAGTGAT TCTTGTGGAA AATGAGAGGT GAGCTCATTC TTCTGAAATG 2951 GTCCCCCTAT CCTGGAAGTC AGTGGGGAGA GGTTTTTGAT TAGACCCCTG 3001 GAGCTATCCG GGTACTCTAA AGGCAAAGCG CACCCCCACT TGGGGACCAA 3051 ACAAAGACCC CTCCGCATTG CAGCCTGCAG TTGCCGCTTC TCAGGTGACG 3101 TGAGGAGGCT GCAACTCAGC ACTAAGTAGT GAAAATGAAA AGCGCCGCTG 3151 TCTGAAATTC ATTAGCAGCC AGAGTATGTG TTACAAGGCA GCGGAGGCTG 3201 GGAGTCTGAA GTGGTGTGAT GAATTGAACC TCATCGGATG CTGCTGTGGC 3251 TGGGCCAAGT GATAGCACCT AATCAATTCC TCACACGTCA AGTGACACCT 3301 CAGACATGGG ATAGATTTCC CCATCACATC ACAGGGCAGG TGCTCCCTCC 3351 CTGCTGGAGA GCACAGGCAC TGCAGAAGCA GCGCACAGTG CCAGGGGCGA 3401 GTGAGGCAGC AGCTCCCAGC CTTTTCAGGC ACGGAGATTG CCTTTCAACA 3451 TCCAAACATT TCCCAGAACC CATGTGCCAT CCTACTTGTA TTACTGGTGG 3501 CCAGAAAGCC ACAAGCGCAA TCATGCTTTT CAATGACCCT ATTTTTATTC 3551 ACGAGAACAG CACATACATG TGTTTGAAAA TTATGTGAGG TGCTCACTCT 3601 GCAGACAGTA CTCACATTCC TATAGATTCC ACCCCTGCCC ACCTTGCAGC 3651 CCCTGGAGTC TATAGCAGAT GGGAGTGGGG CACTCCGAGA GTGGCAGGCC 3701 TGGAGATCAC ATCTTCCATT GTTCCTTCAA TCAACACTAA CTCCCATTTG 3751 GGCCTTAGGT GCCTTGCTAA GCACCACAAA ACAGCAACTA ACTGAAAGAG 3801 ATCTGGAGTG CCAGCCCGCT CCTACTGAGG GCCTCCTCTC TGTCAGGCAC 3851 CTTGCAAAGC ATTTTGTGTG AAGTGACTCA TTTAACCTCA CCACAACGCC 3901 ACAACGCAGG GATTATGCAG GTAACCTATT TCCCAGATGA GGAAGATAAG 3951 GCCCAAGGAG GTGAAATGCC TTTCCCAGAG TTACACAGAG TGCTGGAGCT 4001 GGGAATACTG ACCCAGGCAG TCTAGCTCTT AACAGCTCAC TCCACTGTTT 4051 CCCTGGAGGT GATGCACAGA TGTCACTGGG AAACCCAAAG GAGAGGGGGT 4101 TGGCTGTGTG TGTGTGTGTT GGGCAGGCAG GTAAGGGGAG TAAGACCAGG 4151 ACAAGTGTTC CTGGCAAAGT TCCGGTGACA GCATTAAACA TTCAGATGGT 4201 GAGGGAGTTA ATATGGTTGG AGAACAACAA CTTTAGAGAG AGCAGAGGGG 4251 TCAGTTCACA ACCATCTGCT CAGGAGGGTC AAGATGGGTG GTCTTTATGC 4301 TGAAGGTCTG TGATTAGAGG AGCTGGTTGC TAAATTTTGA GGAGTACCTT 4351 TTGCTCTGTG CTGGACATCT AAATATGCAT GTTAACTGTG TTCTTTAACA 4401 TTTCCAGGAG ACTATAAACA TGTTTGAAAG GAACATTGTT GACATGGTAG 4451 GACTGTTTAT CGAAAATGTC CAAAGCCTAT ATCCTTTCTG TGATGACCTT 4501 CCCCATGGGG AGGTGCTACA GAGCCCCTGG GCTTGTCCCG GCCTCTGGAC 4551 AAAAGAATGT TCCACAGGGT CTGAGGAGGT TTCCCGACCC TCAGAACAAT 4601 GATGGCCTGG TTAGAGCTGT GGTTTGGATG CCCAGAGGGA CAACATCCAA 4651 ACTGTTTGCA GTAGGCTCCC AGCATGATTG TTCTCATATG AGTGATGTTC 4701 ACTAGGAAAT GACGCCCCCT GTGTTGCAGG CAAGCACACT CTGGGGTTGA 4751 GGCAACCCCC ACGTGGAAGA CACTATAAGG AGTACATCAG GTGAAATGTT 4801 AGGGTGAGGA GCCAACATCG GAGCATGGCC AACCCTTCTT CCACCCGAAC 4851 TCAGGGCACT CCACATGGGG CAAACTGCTG TGCTCCAGCT AGCAGCAGCC 4901 CTGTGGTCCT GCCCTCCTGG GGCTCACAGT CCCTCAGGGA GACAAGTTGT 4951 AGAGGCAACA AGTGGTGCCA AATGCACAGG GTGAGAAGCA GTTAACCCAG 5001 AGGCCAGGAG CCTCCATGCA GGAGGGAGAG AAGAGTGTGA TGGCAGGGGC 5051 CGAGGGTCCG TCCGAGGTGT GGGGCAGGGG CAGGGAGTCG AGGAAGGCCC 5101 AGGGTTCGGA GCTTGTGAGT GGACGGTGCT GCCAGCCAGA ATTTCCGAGC 5151 TCGCCTTGGG CCCTTAAAGT CTGTCTCCCG CCGTCTGAGA GCATCAGGGA 5201 CGCGCCGGGC CTGCTCCTCC CGGGCCTTTG CTTAACTCGG GGCTGCACGA 5251 TGGCTCAGTG CCGGGACCTG GAGAATCACC ACCACGAGAA GCTCCTGGAG 5301 ATCTCTATCA GCACCCTGGA GAAGATTGTC GAGGGCGACC TGGACGAGGA 5351 CCTGCCTAAC GACCTGCGCG CGCTTTTTGT CGATAAAGAT ACGATTGTTA 5401 ATGCTGTCGG GGCATCGCAC GACATCCACC TCCTGAAGAT TGACAATCGA 5451 GAAGATGAGC TGGTGACCAG AATCAACTCT TGGTGTACAC GTTTAATAGA 5501 CAGGATTCAC AAGGATGAGA TCATGAGGAA CCGCAAGCGC GTGAAGGAGA 5551 TCAATCAGTA CATCGACCAC ATGCAGAGCG AACTGGACAA CCTGGAATGT 5601 GGCGACATCC TAGACTAGAT GAATGTCAGC CACAGGAGCT TCTTCAAAAC 5651 ATAGCACCAG CCCCAGCCAG GAGAAGGAAG TGCACACGCC TCACCCGCAC 5701 CTCTAGAGAG TTGCTGGGCA TCTCTCAACC GCGATCCCCA ACACCATTCT 5751 TCCCCCACCC CTGGAAAAAC TTCCAAAAGT AGAGAAAATA AAGGACTCAT 5801 TTCACAAAAA AAAAAAAAAA AA BLAST Results Entry HS1292248 from database EMBL: human STS SHGC-53917. Score = 874, P = 3.3e-33, identities = 180/185 Medline entries No Medline entry Peptide information for frame 1 ORF from 202 bp to 876 bp; peptide length: 225 Category: similarity to known protein 1 MNQPCNSMEP RVMDDDMLKL AVGDQGPQEE AGQLAKQEGI LFKDVLSLQL 51 DFRNILRIDN LWQFENLRKL QLDNNIIEKI EGLENLAHLV WLDLSFNNIE 101 TIEGLDTLVN LEDLSLFNNR ISKIDSLDAL VKLQVLSLGN NRIDNMMNII 151 YLRRFKCLRT LSLSRNPISE AEDYKMFICA YLPDLMYLDY RRIDDHTASV 201 SLSVSQPCET DSSSPQVSWK RGIEE BLASTP hits Entry S68209 from database PIR: sds22 protein homolog - human >TREMBL:HSSDS22MR_1 gene: "sds22"; product: "yeast sds22 homolog"; H. sapiens sds22-lιke mRNA Score = 234, P = 1.2e-19, identities = 61/143, positives = 93/143 Entry A38439 from database PIR: suppressor protein sds22(+) - fission yeast (Schizosaccharomyces pombe) >TREMBL:SPSDS22_1 gene: "sds22+"; S. pombe sds22+ gene, complete eds. Score = 208, P = 5.6e-17, identities = 52/127, positives = 71/127 Entry S43988 from database PIR: protein suppressor sds22 - fission yeast (Schizosaccharomyces pombe) >SWISΞPROT:SD22_SCHPO PROTEIN PHOSPHATASES PPl REGULATORY SUBUNIT SDS22. >TREMBL:SPAC4A8_12 gene: "sds22"; product: "phosphatases ppl regulatory subunit"; S. pombe chromosome I cosmid c4A8. Score = 208, P = 8.5e-17, identities = 52/127, positives = 71/127 Entry CEK10D2_5 from database TREMBL: gene: "K10D2.1"; Caenorhabditis elegans cosmid K10D2. Score = 214, P = 3.6e-16, identities = 50/125, positives = 75/125 Alert BLASTP hits for DKFZphutel_20mll, frame 1 No Alert BLASTP hits found Pedant information for DKFZphutel_20mll, frame 1 Report for DKFZphutel_20mll .1 [LENGTH] 225 [MW] 25955 [pi] 4.63 [HOMOL] PIR:S 68209 sds22 protein homolog - human le-18 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YKL193c] 2e-ll [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YKL193c] 2e-ll [FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylation, famesylation and processing) [S. cerevisiae, YKL193c] 2e-ll [FUNCAT] 30.05 organization of centrosome [S. cerevisiae, YOR373w] 2e-06 [FUNCAT] 01.03 .10 metabolism of cyclic and unusual nucleotides [S. cerevisiae, YJL005w] 3e-05 [FUNCAT] 03.10 sporulation and germination [S cerevisiae, YJL005w] 3e-05 [FUNCAT] 30.02 organization of plasma membrane [S. cerevisiae, YJL005w] 3e-05 [FUNCAT] 10.04.03 second messenger formation [S. cerevisiae, YJL005w] 3e-05 [FUNCAT] 04.07 rna transport [S. cerevisiae, YPL169c] 9e-04 [ FUNCAT ] 04.05.01.04 transcriptional control [S. cerevisiae, YCR065w] 9e-04 [EC] 4.6.1.1 Adenylate cyclase 2e-06 [PIRKW] nucleus 5e-16 [PIRKW] duplication 2e-06 [PIRKW] tandem repeat 2e-06 [PIRKW] cAMP biosynthesis 2e-06 [PIRKW] glycoprotein 2e-06 [PIRKW] phosphorus-oxygen lyase 2e-06 [SUPFAM] leucine-rich alpha-2-glycoprotein repeat homology 5e-16 [SUPFAM] fibromodulin 3e-07 [SUPFAM] yeast adenylate cyclase catalytic domain homology 2e-06 [SUPFAM] yeast adenylate cyclase 2e-06 [PROSITE] CK2_PHOSPHO_SITE 2 [PROSITE] PKC PHOSPHO SITE 1 [KW] All_Alpha SEQ MNQPCNSMEPRVMDDDMLKLAVGDQGPQEEAGQLAKQEGILFKDVLSLQLDFRNILRIDN PRD ccccccccccccccchhhhhhcccccchhhhhhhhhhhchhhhhhhhhcccccccccccc SEQ LWQFENLRKLQLDNNIIEKIEGLENLAHLVWLDLSFNNIETIEGLDTLVNLEDLSLFNNR PRD hhhhhhhhhhhhcccccccccccchhhhhhhhcccccccccccccchhhhhhhhhccccc SEQ ISKIDΞLDALVKLQVLSLGNNRIDNMMNIIYLRRFKCLRTLSLSRNPISEAEDYKMFICA PRD cccchhhhhhhhhhhhhccccccccccccccchhhhhhhhhcccccccccchhhhhhhhh SEQ YLPDLMYLDYRRIDDHTASVSLSVSQPCETDSSSPQVSWKRGIEE PRD hhcccccccccccccchhhhhhhhccccccccccccccccccccc Prosite for DKFZphutel_20mll .1 PS00005 218->221 PKC_PHOSPHO_SITE PDOC00005 PS00006 122->126 CK2_PHOSPHO_SITE PDOC00006 PS00006 169->173 CK2 PHOSPHO SITE PDOC00006 (No Pfam data available for DKFZphutel_20mll .1) DKFZphutel_20m24 group: metabolism DKFZphutel_20m24 encodes a novel 611 amino acid protein with similarity to a hypothetical C. elegans protein and to yeast Alg9 protein. This protein is a putative mannosyl transferase that is involved in the assembly of the core oligosaccharide Glc3Man9GlcNAc2. The new protein can find application in modulation of glycosylation of proteins and as a new enzyme for biotechnologic production processes. strong similarity to S. cerevisiae Alg9p complete cDNA, complete eds, potential start at Bp 23, few EST hits Alg9 is involved in the assembly of the core oligosaccharide Glc3Man9GlcNAc2 HSAC381 corresponding genomic DNA (2 exons) HSB8954 corresponding genomic DNA (1 exon ) Sequenced by AGOWA Locus: /map="ll" Insert length: 1986 bp Poly A stretch at pos. 1966, polyadenylation signal at pos. 1949 1 TTCTTTTTTC CCCAGGCTTG CCATGGCTAG TCGAGGGGCT CGGCAGCGCC 51 TGAAGGGCAG CGGGGCCAGC AGTGGGGATA CGGCCCCGGC TGCGGACAAG 101 CTGCGGGAGC TGCTGGGCAG CCGAGAGGCG GGCGGCGCGG AGCACCGGAC 151 CGAGTTATCT GGGAACAAAG CAGGACAAGT CTGGGCACCT GAAGGATCTA 201 CTGCTTTCAA GTGTCTGCTT TCAGCAAGGT TATGTGCTGC TCTCCTGAGC 251 AACATCTCTG ACTGTGATGA AACATTCAAC TACTGGGAGC CAACACACTA 301 CCTCATCTAT GGGGAAGGGT TTCAGACTTG GGAATATTCC CCAGCATATG 351 CCATTCGCTC CTATGCTTAC CTGTTGCTTC ATGCCTGGCC AGCTGCATTT 401 CATGCAAGAA TTCTACAAAC TAATAAGATT CTTGTGTTTT ACTTTTTGCG 451 ATGTCTTCTG GCTTTTGTGA GCTGTATTTG TGAACTTTAC TTTTACAAGG 501 CTGTGTGCAA GAAGTTTGGG TTGCACGTGA GTCGAATGAT GCTAGCCTTC 551 TTGGTTCTCA GCACTGGCAT GTTTTGCTCA TCATCAGCAT TCCTTCCTAG 601 TAGCTTCTGT ATGTACACTA CGTTGATAGC CATGACTGGA TGGTATATGG 651 ACAAGACTTC CATTGCTGTG CTGGGAGTAG CAGCTGGGGC TATCTTAGGC 701 TGGCCATTCA GTGCAGCTCT TGGTTTACCC ATTGCCTTTG ATTTGCTGGT 751 CATGAAACAC AGGTGGAAGA GTTTCTTTCA TTGGTCGCTG ATGGCCCTCA 801 TACTATTTCT GGTGCCTGTG GTGGTCATTG ACAGCTACTA TTATGGGAAG 851 TTGGTGATTG CACCACTCAA CATTGTTTTG TATAATGTCT TTACTCCTCA 901 TGGACCTGAT CTTTATGGTA CAGAACCCTG GTATTTCTAT TTAATTAATG 951 GATTTCTGAA TTTCAATGTA GCCTTTGCTT TGGCTCTCCT AGTCCTACCA 1001 CTGACTTCTC TTATGGAATA CCTGCTGCAG AGATTTCATG TTCAGAATTT 1051 AGGCCACCCG TATTGGCTTA CCTTGGCTCC AATGTATATT TGGTTTATAA 1101 TTTTCTTCAT CCAGCCTCAC AAAGAGGAGA GATTTCTTTT CCCTGTGTAT 1151 CCACTTATAT GTCTCTGTGG CGCTGTGGCT CTCTCTGCAC TTCAGAAATG 1201 TTACCACTTT GTGTTTCAAC GATATCGCCT GGAGCACTAT ACTGTGACAT 1251 CGAATTGGCT GGCATTAGGA ACTGTCTTCC TGTTTGGGCT CTTGTCATTT 1301 TCTCGCTCTG TGGCACTGTT CAGAGGATAT CACGGGCCCC TTGATTTGTA 1351 TCCAGAATTT TACCGAATTG CTACAGACCC AACCATCCAC ACTGTCCCAG 1401 AAGGCAGACC TGTGAATGTC TGTGTGGGAA AAGAGTGGTA TCGATTTCCC 1451 AGCAGCTTCC TTCTTCCTGA CAATTGGCAG CTTCAGTTCA TTCCATCAGA 1501 GTTCAGAGGT CAGTTACCAA AACCTTTTGC AGAAGGACCT CTGGCCACCC 1551 GGATTGTTCC TACTGACATG AATGACCAGA ATCTAGAAGA GCCATCCAGA 1601 TATATTGATA TCAGTAAATG CCATTATTTA GTGGATTTGG ACACCATGAG 1651 AGAAACACCC CGGGAGCCAA AATATTCATC CAATAAAGAA GAATGGATCA 1701 GCTTGGCCTA TAGACCATTC CTTGATGCTT CTAGATCTTC AAAGCTGCTG 1751 CGGGCATTCT ATGTCCCCTT CCTGTCAGAT CAGTATACAG TGTACGTAAA 1801 CTACACCATC CTCAAACCCC GGAAAGCAAA GCAAATCAGG AAGAAAAGTG 1851 GAGGTTAGCA ACACACCTGT GGCCCCAAAG GACAACCATC TTGTTAACTA 1901 TTGATTCCAG TGACCTGACT CCCTGCAAGT CATCGCCTGT AACATTTGTA 1951 ATAAAGGTCT TCTGACATGA AAAAAAAAAA AAAAAA BLAST Results Entry HSAC381 from database EMBL: Homo sapiens chromosome 11 pac pDJ159ol, complete sequence. Length = 42,771 Entry HSB8954 from database EMBL: cSRL-50A3-u cSRL flow sorted Chromosome 11 specific cosmid Homo sapiens genomic clone CSRL-50A3. Length = 601 Medl e entries 96293493: Stepwise assembly of the lipid-l nked oligosaccharide in the endoplasmic reticulum of Saccharomyces cerevisiae: identification of the ALG9 gene encoding a putative mannosyl transferase. Peptide information for frame 2 ORF from 23 bp to 1855 bp; peptide length: 611 Category: strong similarity to known protein 1 MASRGARQRL KGSGASSGDT APAADKLREL LGSREAGGAE HRTELSGNKA 51 GQVWAPEGST AFKCLLSARL CAALLSNIΞD CDETFNYWEP THYLIYGEGF 101 QTWEYSPAYA IRSYAYLLLH AWPAAFHARI LQTNKILVFY FLRCLLAFVS 151 CICELYFYKA VCKKFGLHVS RMMLAFLVLS TGMFCSSSAF LPSSFCMYTT 201 LIAMTGWYMD KTSIAVLGVA AGAILGWPFS AALGLPIAFD LLVMKHRWKS 251 FFHWSLMALI LFLVPVVVID SYYYGKLVIA PLNIVLYNVF TPHGPDLYGT 301 EPWYFYLING FLNFNVAFAL ALLVLPLTSL MEYLLQRFHV QNLGHPYWLT 351 LAPMYIWFII FFIQPHKEER FLFPVYPLIC LCGAVALSAL QKCYHFVFQR 401 YRLEHYTVTS NWLALGTVFL FGLLSFSRSV ALFRGYHGPL DLYPEFYRIA 451 TDPTIHTVPE GRPVNVCVGK EWYRFPSSFL LPDNWQLQFI PSEFRGQLPK 501 PFAEGPLATR IVPTDMNDQN LEEPSRYIDI SKCHYLVDLD TMRETPREPK 551 YSSNKEEWIS LAYRPFLDAS RSSKLLRAFY VPFLSDQYTV YVNYTILKPR 601 KAKQIRKKSG G BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphutel_20m24, frame 2 SWISSPROT :YTH3_CAEEL HYPOTHETICAL 75.5 KD PROTEIN C14A4.3 IN CHROMOSOME II., N = 1, Score = 957, P = 2.7e-96 PIR:Ξ63177 mannosyl transferase (EC 2.4.1.-) - yeast (Saccharomyces cerevisiae), N = 1, Score = 533, P = 2.3e-51 SWISSPROT :YTH3_CAEEL HYPOTHETICAL 75.5 KD PROTEIN C14A4.3 IN CHROMOSOME II., N = 1, Score = 957, P = 2.7e-96 PIR:S63177 mannosyl transferase (EC 2.4.1.-) - yeast (Saccharomyces cerevisiae), N = 1, Score = 533, P = 2.3e-51 >SWISSPROT:YTH3_CAEEL HYPOTHETICAL 75.5 KD PROTEIN C14A4.3 IN CHROMOSOME II. Length = 653 HSPs: Score = 957 (143.6 bits), Expect = 2.7e-96, P = 2.7e-96 Identities = 206/514 (40%), Positives = 296/514 (57%) Query: 48 NKAGQVWAPEGSTAFKCLLSARLCAALLSNISDCDETFNYWEPTHYLIYGEGFQTWEYSP 107 N W + FK LLS R+ A+ I+DCDE +NYWEP H +YGEGFQTWEYSP Sbjct: 43 NNPDNDWPFSFGSVFKMLLSIRISGAIWGIINDCDEVYNYWEPLHLFLYGEGFQTWEYSP 102 Query: 108 AYAIRSYAYLLLHAWPAAFHARILQTNKILVFYFLRCLLAFVSCICELYFYKAVCKKFGL 167 YAIRSY Y+ LH PA+ A + KI+VF +R + + E Y + A+CKK + Sbjct: 103 VYAIRSYFYIYLHYIPASLFANLFGDTKIVVFTLIRLTIGLFCLLGEYYAFDAICKKINI 162 Query: 168 HVSRMMLAFLVLSTGMFCSSSAFLPSSFCMYTTLIAMTGWYMDKTSIAVLGVAAGAILGW 227 R + F + S+GMF +S+AF+PSSFCM T + + + + + VA ++GW Sbjct: 163 ATGRFFILFSIFSSGMFLASTAFVPSSFCMAITFYILGAYLNENWTAGIFCVAFSTMVGW 222 Query: 228 PFSAALGLPIAFDLLVMKHRWKSFFHWSLMALILFLVPVVVIDSYYYGKLVIAPLNIVLY 287 PFSA LGLPI D+L++K F SL+ + V+ DS+Y+GK V+APLNI LY Sbjct: 223 PFSAVLGLPIVADMLLLKGLRIRFILTSLVIGLCIGGVQVITDSHYFGKTVLAPLNIFLY 282 Query: 288 NVFTPHGPDLYGTEPWYFYLINGFLNFNVAFALALLVLPLTSLMEYLLQRFHVQNLGHPY 347 NV + GP LYG EP FY+ N F N+N+ A PL+ + Y + + Q+ Sbjct: 283 NVVSGPGPSLYGEEPLSFYIKNLFNNWNIVIFAAPFGFPLS--LAYFTKVWMΞQDRNVAL 340 Query: 348 WLTLAPMYI WFIIFFIQPHKEERFLFPVYPLICLCGAVALSALQKCYHFVFQR 400 + AP+ + W +IF Q HKEERFLFP+YP I A+AL A + ++ Sbjct: 341 YQRFAPIILLAVTTAAWLLIFGSQAHKEERFLFPIYPFIAFFAALALDATNR LCLKK 397 Query: 401 YRLEHYTVTSNWLALGTVFLFGLLSFSRSVALFRGYHGPLDLYPEFYRIATDPTIHTVPE 460 ++ N L++ + F +LS SR+ ++ Y +++Y T+ T + Sbjct: 398 LGMD NILSILFILCFAILSAΞRTYSIHNNYGSHVEIYRSLNAELTNRT-NFKNF 450 Query: 461 GRPVNVCVGKEWYRFPSSFLLPDNW QLQFIPSEFRGQLPKPFAEGPL ATRI 511 P+ VCVGKEW+RFPSSF +P +++FI SEFRG LPKPF + TR Sbjct: 451 HDPIRVCVGKEWHRFPSSFFIPQTVSDGKKVEMRFIQSEFRGLLPKPFLKSDKLVEVTRH 510 Query: 512 VPTDMNDQNLEEPSRYIDIΞKCHYLVDLDTMRETPREPKYSSNKEEW 558 +PT+MN+ N EE SRY+D+ C Y+VD+D M ++ REP + ++ + Sbjct: 511 IPTEMNNLNQEEISRYVDLDSCDYVVDVD-MPQSDREPDFRKMRQNY 556 Pedant information for DKFZphutel_20m24, frame 2 Report for DKFZphutel_20m24.2 [LENGTH] 611 [MW] 69863.78 [pi] 8.91 [HOMOL] SWISSPROT :YTH3_CAEEL HYPOTHETICAL 75.5 KD PROTEIN C14A4.3 IN CHROMOSOME II. 2e- 93 [FUNCAT] 09.01 biogenesis of cell wall [S. cerevisiae, YNL219c] 4e-69 [FUNCAT] 01.06.01 lipid, fatty-acid and sterol biosynthesis [S. cerevisiae, YNL219c] 4e-69 [FUNCAT] 01.05.01 carbohydrate utilization [S. cerevisiae, YNL219c] 4e-69 [PIRKW] glycosyltransferase 9e-68 [PIRKW] transmembrane protein 9e-68 [PIRKW] hexosyltransferase 9e-68 [PROSITE] MYRISTYL 9 [PROSITE] CAMP_PHOSPHO_SITE 1 [PROSITE] CK2_PHOSPHO_SITE 7 [PROSITE] PKC_PHOSPHO_SITE 6 [PROSITE] ASN_GLYCOSYLATION 2 [KW] TRANSMEMBRANE 7 [KW] LOW COMPLEXITY 6.71 % SEQ MASRGARQRLKGSGASSGDTAPAADKLRELLGSREAGGAEHRTELΞGNKAGQVWAPEGST SEG PRD ccchhhhhhhcccccccccccchhhhhhhhhccccccccccceeecccccccccccccch MEM MMMMMM SEQ AFKCLLSARLCAALLΞNISDCDETFNYWEPTHYLIYGEGFQTWEYSPAYAIRSYAYLLLH SEG ... xxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhccccceeeccccceeeeeccccceeecccchhhhhhhhhhhc MEM MMMMMMMMMMMMMMMMM M SEQ AWPAAFHARILQTNKILVFYFLRCLLAFVSCICELYFYKAVCKKFGLHVSRMMLAFLVLS SEG PRD cchhhhhhhhhcchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcc MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMM SEQ TGMFCSSSAFLPSSFCMYTTLIAMTGWYMDKTSIAVLGVAAGAILGWPFSAALGLPIAFD SEG xxxxxxxxxxxxx PRD cceeeeccccccchhhhhhhhhhhhcccccccceeeeeehhhhhhccceeeeeecchhhh MEM MMMMMMMMMMMMMM SEQ LLVMKHRWKSFFHWSLMALILFLVPVVVIDSYYYGKLVIAPLNIVLYNVFTPHGPDLYGT SEG PRD hhhhhhhhhhhhhhhhhhhhhheeeeeeeecccccccccccceeeeeeeecccccccccc MEM MMMMMMM. MMMMMMMMMMMMMMMMMMMMM SEQ EPWYFYLINGFLNFNVAFALALLVLPLTΞLMEYLLQRFHVQNLGHPYWLTLAPMYIWFII SEG xxxxxxxxxxxxxxx PRD cceeeeeecccccchhhhhhhhhhhhchhhhhhhhhhhhccccccceeeeehhhhhhhhh MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM SEQ FFIQPHKEERFLFPVYPLICLCGAVALΞALQKCYHFVFQRYRLEHYTVTSNWLALGTVFL SEG PRD hhcccchhhhhhcccceeehhhhhhhhhhhhhhhhhhhhhhhhheeeeccchhhhhhhee MEM MMMMMMMMMMMMMMMMMMMMM MMMMMMMMMMMMMMMMMMMMM . SEQ FGLLSFSRSVALFRGYHGPLDLYPEFYRIATDPTIHTVPEGRPVNVCVGKEWYRFPSSFL SEG PRD eehhhhhhhheeecccccccccccceeeeccccccceeecccceeeeeeccccccccccc MEM SEQ LPDNWQLQFIPSEFRGQLPKPFAEGPLATRIVPTDMNDQNLEEPSRYIDISKCHYLVDLD SEG PRD ccccceeeecccccccccccccccccceeeeccccccccccccccceeeeeeceeeeecc MEM SEQ TMRETPREPKYSSNKEEWISLAYRPFLDASRSSKLLRAFYVPFLSDQYTVYVNYTILKPR SEG PRD cccccccccccchhhhhhhhhhhhhhhhhhhhhhheeeeeeeeecceeeeeeeeeecccc MEM SEQ KAKQIRKKSGG SEG PRD hhhhhhccccc MEM Prosite for DKFZphutel_20m2 .2 PS00001 77->81 ASN_GLYCOSYLATION PDOC00001 PS00001 593->597 ASN_GLYCOSYLATION PDOC00001 PS00004 606->610 CAMP_PHOSPHO_SITE PDOC00004 PS00005 67->70 PKC_PHOSPHO_SITE PDOC00005 PS00005 133->136 PKC_PHOSPHO_SITE PDOC00005 PS00005 541->544 PKC_PHOSPHO_SITE PDOC00005 PS00005 545->548 PKC_PHOSPHO_SITE PDOC00005 PS00005 553->556 PKC_PHOSPHO_SITE PDOC00005 PS00005 572->575 PKC_PHOSPHO_SITE PDOC00005 PS00006 16->20 CK2_PHOSPHO_SITE PDOC00006 PS00006 79->83 CK2_PHOSPHO_SITE PDOC00006 PS00006 329->333 CK2_PHOSPHO_SITE PDOC00006 PS00006 457->461 CK2_PHOSPHO_SITE PDOC00006 PS00006 541->545 CK2_PHOSPHO_SITE PDOC00006 PS00006 545->549 CK2_PHOSPHO_SITE PDOC00006 PS00006 553->557 CK2_PHOSPHO_SITE PDOC00006 PS00008 12->18 MYRISTYL PDOC00008 PS00008 14->20 MYRISTYL PDOC00008 PS00008 32->38 MYRISTYL PDOC00008 PS00008 47->53 MYRISTYL PDOC00008 PS00008 166->172 MYRISTYL PDOC00008 PS00008 182-M88 MYRISTYL PDOC00008 PS00008 218->224 MYRISTYL PDOC00008 PS00008 222->228 MYRISTYL PDOC00008 PΞ00008 234->240 MYRISTYL PDOC00008 (No Pfam data available for DKFZphutel 20m24.2) DKFZphutel_21dl5 group: uterus derived DKFZphutel_21dl5 encodes a novel 191 amino acid protein without similarity to known proteins. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application m studying the expression profile of testis-specific genes . unknown Sequenced by MediGenomix Locus: /chromosome="3" Insert length: 5292 bp Poly A stretch at pos. 5273, polyadenylation signal at pos. 5252 1 CTCCCACTAG TGTATGCCTT AATGGTGCCG CTCTTGTCCG CGTCTACGCT 51 TGGGACCTTG GCTTCTGACT TGGAGAGTGT ACAGCTCTGC CCGACGGCAA 101 CCCAGCTTGG GAAGAGAAGC CCCAGCGTGG GCTGGGGCTC AAGGCGCAGG 151 AAGGCCGAGC CCGGCGCGGA CGCAGGCGGC TCCGGGCGGG CTCAGCACCC 201 CCAGGCACCG TCTCCTAGTG ACCGCGGCGC TCGCGGGCCT GGCGGCCGTT 251 GTCCGGGCGA CTGCGCAGCG CGGGCACCCC CGCGGCCCCT CCCCTGGGCG 301 CGCGCGCGAC CTGGGTGCCA TGGCGGCAGC GGCGGTGACA GGCCAGCGGC 351 CTGAGACCGC GGCGGCCGAG GAGGCCTCGA GGCCGCAGTG GGCGCCGCCA 401 GACCACTGCC AGGCTCAGGC GGCGGCCGGG CTGGGCGACG GCGAGGACGC 451 ACCGGTGCGT CCGCTGTGCA AGCCCCGCGG CATCTGCTCG CGCGCCTACT 501 TCCTGGTGCT GATGGTGTTC GTGCACCTGT ACCTGGGTAA CGTGCTGGCG 551 CTGCTGCTCT TCGTGCACTA CAGCAACGGC GACGAAAGCA GCGATCCCGG 601 GCCCCAACAC CGTGCCCAGG GCCCCGGGCC CGAGCCCACC TTAGGTCCCC 651 TCACCCGGCT GGAGGGCATC AAGGTGAGGA CCTCCCTGCC CCGCCGCGCT 701 CCAGGCCCTG CACGGCTGAG CCCGAGAGGA CCGGCGCTCA GCCCGGGTCC 751 CCACGCTGCC CCCGGCGCTG CTCTGCGTCG GTCCCGCGCG CTCCCACTCA 801 CTCGCCTGCT GTCGCTCTCC GGGCCGGGGC GACTTGGCCC TTTTTGGGCA 851 GCGCGGTCTG GCGCCCCAGC TGCCCGCTGT GCGCCTTTTC CTTAGGTGGG 901 GCACGAGCGT AAGGTCCAGC TGGTCACCGA CAGGGATCAC TTCATCCGAA 951 CCCTCAGCCT CAAGCCGCTG CTCTTCGAAA TCCCCGGCTT CCTGACTGAT 1001 GAAGAGTGTC GGCTCATCAT CCATCTGGCG CAGATGAAGG GGTTACAGCG 1051 CAGCCAGATC CTGCCTACTG AAGAGTATGA AGAGGCAATG AGCACTATGC 1101 AGGTCAGCCA GCTGGACCTC TTCCGGCTGC TGGACCAGAA CCGTGATGGG 1151 CACCTTCAGC TCCGTGAGGT TCTGGCCCAG ACTCGCCTGG GAAATGGATG 1201 GTGGATGACT CCAGAGAGCA TTCAGGAGAT GTACGCCGCG ATCAAGGCTG 1251 ACCCTGATGG TGACGGTGAG CTCACACCTC TGCACAGTCC TATCCCCGTG 1301 AGCCTCCTGC CCACTCCCAG GTGCACAATT TTGAAAACTT GGGCCCTTCC 1351 CCCACAGCCA GGCAGCCTCT CTGCACCCCT TTATAGTGGC CAGAGATGGG 1401 GAGGTGAAGA TCCAGCCTTG CTTTTTACCC CTGGGAAGTA GGCAGGCAGC 1451 CAGGCCCCCC GTTCCCCTTG GTGATGGTCT CGAGGGCAGT TCTTGGAGAC 1501 CCTTTTGATA ACATCAGGCA GAGTTGAGAG CCTGGGGACA GGAAGTAGGG 1551 CTGCTAGTTG GCAGAGAACA GAGTGGGTGG AGCAGGAGCA AGGCGACAGT 1601 GAGGCCAGCT AGAGCTTGGC TGTTTACCCT GCTCCATCCA TCTCTCCAGC 1651 CAGACACGAG GTCCACCCCA GCAGACAGCT TCCCTGGTCT AAGTGAGGTC 1701 TCCCTTGCCT TCCTCTTGTC CACCTGGAGT CATGCCGAAG CGCCTAAAAT 1751 GGTAGTGCTG CTACCTGTGC TAACTGCTGG GGAGGGGTGG GCAGGGAAGC 1801 TGTCATGCAA GTGGTGCCCC CTCTGGTAAT AACTCTCAGG AGGTTTCTGA 1851 GGTGTGGTCA TCACCCTCAT GCCCAAATTC TGGACCAAGA GAGGAAGATA 1901 CAGCAGTTAG AAAGGACTTG GAACAGTGGC TTTGCGGCTG GTGAACCAGA 1951 GTGAAGAATC TGGCCGTGAC CTGGCTGCCA CACTGCTATA GGCCCCAGAA 2001 CAGAGGTGGT GACAGTCTCA CAGCCCTTGA ATGTCCCCCA CCCTCAGAGG 2051 AATCTGGGCC AAAGAGTGGA AGGTGATGTC CTTGGGTCAG CCAGAATAAC 2101 ATGGAGCAAA GATACCAACT ACTCTTCCAG AACCCCAAGA GGGTAGAACC 2151 CCTGCTTAAT GGTTTGAGCA GGGACAGTGG AGAATGTTCT CATGAGAGGG 2201 GGTGGCCTGA CTTTCGTTGC TAAGTGGGCT GGTAACGCAG TAGGCAGGGC 2251 TGGCGAAGTA GGTTCCACCC AGGATGAAAC CTGGGGTCAT GAGGAACTCC 2301 CCGGGGGCTG GCCCTGCTTG CACCCTGGCG TATGTATGTA AGGCCCTGGA 2351 TGAGGCCCAG CACTGCCTGC TCTCTCCTCA CCCTCCACAG GCCGGAGAGT 2401 GGCCACCACT CTATATAGCC AGGCTGGAAG GCCAGGGTCC TGGCCATATG 2451 GCTCAAGCTT CCTTTGGAGA ACCTTCTCTG GCCACTCTAA TAGGGGGTGG 2501 GCCTCTTTCT TCTTAGGGCC AAATTAGGGC TTAAACTGAG AAAAGGAACT 2551 GCTCTGGGTC TTCCTGTAAG GCCTGATGTG ACAGAAACCA GGTTCATCTG 2601 ACCCAAAAGT CCAGGTGGGG GACAAGTGTA CAAGGCCCCT CAGTGCCTGA 2651 GGTCAGGGGC TGCTGCTGCC TTTGGGGTAG GTAGGGAAGT GCAGCCTGCC 2701 ACTGTTGCCT CCCAATATGG GCTTGGTGGG CATTGATGGT GGGTGCCCTG 2751 TGCAGGAGTG CTGAGTCTGC AGGAGTTCTC CAACATGGAC CTTCGGGACT 2801 TCCACAAGTA CATGAGGAGC CACAAGGCAG AGTCCAGTGA GCTGGTGCGG 2851 AACAGCCACC ATACCTGGCT CTACCAGGGT GAGGGTGCCC ACCACATCAT 2901 GCGTGCCATC CGCCAGAGGT GAGCACCTGA AGCTGTTCTC ACTGGAGCAG 2951 GGGGAGAAGA CTGGGCAGGG CCTCCACAGA AGTCCTTGTC TGGGGCCAAG 3001 AGGACAGAAT GGATTAACCC ATTTGGGATT AAGTTCCATT TGTTAGACCA 3051 GGATTGGGAC CCACTGAAAG ACAGGCAATT AACAAAGGCA AATTAGCCCT 3101 CCTTGCAGGC ACACAATGGG CAACTGGGGT TAGATAGAGA TTGAGCACTT 3151 CTTTCTGATT AGATAAATGA CCTCTTATCT TTGACCCCTT ATCTGACCCC 3201 GTCACAGCAG GAAAAGGGTT TTTAAATAAA CAACTTTCTT CCAGGGAGGA 3251 GGACCTCAGG ACTCCCCGCC CCCTTTATTT AGTGGAAATG TCAACATTTC 3301 CACATAGCAG GTGTCTCTGT CTTTGGCATC TGAGGGAGAA GGATCATCAT 3351 GAGTAACCCC CTCCTGCTCT TACAGGGCCA GTCTGAGATG GCTTAAGGGA 3401 CTTCCAGGGG AGGTGGGTAG GGGCAAAGCT TGTGGCAGGC CTAGGGTCCA 3451 CCTTGGCCAG CTCCTTCAGA TCACCACCTT GCCTGGGGCT GCCCAGCCAA 3501 ATGCCTGCTG CCCACCAGGG TGCTGCGCCT CACTCGCCTG TCGCCTGAGA 3551 TCGTGGAGCT CAGCGAGCCG CTGCAGGTTG TTCGATATGG TGAGGGGGGC 3601 CACTACCATG CCCACGTGGA CAGTGGGCCT GTGTACCCAG AGACCATCTG 3651 CTCCCATACC AAGCTGGTAG CCAACGAGTC TGTACCCTTC GAGACCTCCT 3701 GCCGGCAAGT ATCTCCCAAC TGGGGGCTGC CTTCAATCCT CAGACCAGGA 3751 ACACCCATGA CACAGGCACA GCCCTGCACT GTGGGCGTGC CCCTTGGCAT 3801 GGGGCCAGGA GATCACTGGG TTATCCCGGT TAGTGATGCC CTCACCTCTC 3851 CCCACAAGTT GTTTACCCAA TGGCTGGAAA GGGGTGGCTA CTGGTCATCG 3901 TGACCACTGG AGTCAACACA GACTGATGTA CCCACAGACA CCAAAACTTG 3951 CCCCCTGAGT TCTGAAGCAA GGGGCAAGGC TGGGCCCCTA GCTTGTCCTG 4001 CCCATTCCTC CAGGTGTTGA TCTTGATTCC ACTTAGAGAA GCTGAAGCTG 4051 TGCCTCCCTC CCCTGTCAAG CCAGTTCTTT CCTCTTCAGG TGGCTGTTCT 4101 GGCCCAGCCC CTTCCCATCC CCAAGGAGCC CTTCAGCGCG CCCTGTTGCT 4151 TCTGCTAGCC TACCTTTCCC TGCCAGGCCC TTGCTCAGGG CCATGGCATT 4201 TAACTAAGTG CACCTGTGAT CTTGGCCAAA AAACCATTGC AACTCACAGT 4251 AAGAGACTGG GTTTCGGGGA AGGAGGGGCT AGGGACATTT TGGCACTGGC 4301 CTGCCCTATT GTCTCCCATC CTAGTCTGTC CTGGTCCCTG GCAACAGGAA 4351 CCTGGGCAGC TTATCCTGCC CACAGGTAAG CCCCTGGGAG CATCCACAAC 4401 TGGGGACCTG CTCAGTGCCC CCCCTGCCTT ACAGCTACAT GACAGTGCTG 4451 TTTTATTTGA ACAACGTCAC TGGTGGGGGC GAGACTGTTT TCCCTGTAGC 4501 AGATAACAGA ACCTACGATG AAATGGTAAG GGTCAACTGG GCTATTACTC 4551 TTGTGGGCTG GCAGGGGCTT AGACAAGTGA AGTACACACC TCTCCAGGTC 4601 TAAGGATGTG GGCCCAAATT ATTCCTTGGG CATATCTGGT TGGTTTCCCT 4651 TTGGTCACCC TTGGCTGGCC TGGCCATAGA GTGGGGACAG GTTGAACACC 4701 CCACCACCCT GCTGCCCACA GAGTCTGATT CAGGATGACG TGGACCTCCG 4751 TGACACACGG AGGCACTGTG ACAAGGGAAA CCTGCGTGTC AAGCCCCAAC 4801 AGGGCACAGC AGTCTTCTGG TACAACTACC TGCCTGATGG GCAAGGTTGG 4851 GTGGGTGACG TAGACGACTA CTCGCTGCAC GGGGGCTGCC TGGTCACGCG 4901 CGGCACCAAG TGGATTGCCA ACAACTGGAT TAATGTGGAC CCCAGCCGAG 4951 CGCGGCAAGC GCTGTTCCAA CAGGAGATGG CCCGCCTTGC CCGAGAAGGG 5001 GGCACCGACT CACAGCCCGA GTGGGCTCTG GACCGGGCCT ACCGCGATGC 5051 GCGCGTGGAA CTCTGAGGGA AGAGTTAGCC CCGGTTCCCA GCCGCGGGTC 5101 GCCAGTTGCC CAAGATCAGG GGTCCGGCTG TCCTTCTGTC CTGCTGCAGA 5151 CTAAAGGTCT GGCCAATGTC TTGCCCCACC CCGCCAGCCG CGATACGGCG 5201 CAGTTCCTAT ATTCATGTTA TTTATTGTGT ACTGACTCCA TCTGCCCCGT 5251 CAAATAAAAA ACCACAAGGT TCGAAAAAAA AAAAAAAAAA GG BLAST Results Entry HSU64252 from database EMBL: Human STS sequence NOTI-225. Score = 959, P = 1.2e-36, identities = 195/199 Medline entries No Medline entry Peptide information for frame 1 ORF from the beginning to 351 bp; peptide length: 118 Category: questionable ORF Classification: no clue 1 LPLVYALMVP LLSASTLGTL AΞDLESVQLC PTATQLGKRS PSVGWGSRRR 51 KAEPGADAGG ΞGRAQHPQAP SPSDRGARGP GGRCPGDCAA RAPPRPLPWA 101 RARPGCHGGS GGDRPAA BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphutel_21dl5, frame 1 No Alert BLASTP hits found Peptide information for frame 2 ORF from 320 bp to 892 bp; peptide length: 191 Category: putative protein Classification: no clue 1 MAAAAVTGQR PETAAAEEAS RPQWAPPDHC QAQAAAGLGD GEDAPVRPLC 51 KPRGICSRAY FLVLMVFVHL YLGNVLALLL FVHYSNGDES SDPGPQHRAQ 101 GPGPEPTLGP LTRLEGIKVR TSLPRRAPGP ARLSPRGPAL SPGPHAAPGA 151 ALRRSRALPL TRLLSLSGPG RLGPFWAARS GAPAARCAPF P BLASTP hits No BLASTP h ts available Alert BLASTP hits for DKFZphutel_21dl5, frame 2 PIR:EDBE75 immediate-early protein IE175 - human herpesvirus 1, N = 2, Score = 106, P = 0.0067 >PIR:EDBE75 immediate-early protein IE175 - human herpesvirus 1 Length = 1,298 HSPs: Score = 106 (15.9 bits), Expect = 6.7e-03, Sum P(2) = 6.7e-03 Identities = 36/103 (34%), Positives = 44/103 (42%) Query 87 GDESSDPGPQHRAQGPGPEPTLGPLTRLEGIKVRTSLPRRA-PGPARLS-PRGPALSPGP 144 G + PGP G GP P P T+ G S R P PA S P GP +P Sbjct 726 GRKRKSPGPARPPGGGGPRP PKTKKSGADAPGSDARAPLPAPAPPSTPPGPEPAPAQ 782 Query 145 HAAPGAALRRSRALPLT-RLLSLSGPGRLGPFWAARSGAPAARCAP 189 AAP AA ++R P+ GP LG W + P+ AP Sbjct 783 PAAPRAAAAQARPRPVAVSRRPAEGPDPLGG-WRRQPPGPΞHTAAP 827 Score = 40 (6.0 bits), Expect = 6.7e-03, Sum P(2) = 6.7e-03 Identities = 8/21 (38%), Positives = 9/21 (42%) Query: 28 DHCQAQAAAGLGDGEDAPVRP 48 DH + A G G AP P Sbjct: 212 DHAREARAVGRGPSSAAPAAP 232 Pedant information for DKFZphutel_21dl5, frame 1 Report for DKFZphutel_21dl5.1 [LENGTH] 117 [MW] 11797.32 [pi] 10.68 [KW] Irregular [KW] SIGNAL PEPTIDE 22 [KW] LOW COMPLEXITY 38.46 % SEQ LPLVYALMVPLLSASTLGTLASDLESVQLCPTATQLGKRΞPSVGWGSRRRKAEPGADAGG SEG xxxxxxxxxxxxxx PRD cccccccccccccccccccchhhhhhhhcccccccccccccccccccccccccccccccc SEQ SGRAQHPQAPSPSDRGARGPGGRCPGDCAARAPPRPLPWARARPGCHGGSGGDRPAA SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD ccccccccccccccccccccccccccccccccccccccccccccccccccccccccc (No Prosite data available for DKFZphutel_21dl5.1) (No Pfam data available for DKFZphutel_21dl5.1) Pedant information for DKFZphutel_21dl5, frame 2 Report for DKFZphutel_21dl5.2 [LENGTH] 191 [MW] 19916.88 [pi] 10.43 [KW] TRANSMEMBRANE 1 [KW] LOW_COMPLEXITY 29.84 % SEQ MAAAAVTGQRPETAAAEEASRPQWAPPDHCQAQAAAGLGDGEDAPVRPLCKPRGICSRAY SEG PRD ccceeeeccccchhhhhhhhhccccccchhhhhhhhcccccccccccccccccccchhhh MEM SEQ FLVLMVFVHLYLGNVLALLLFVHYSNGDESSDPGPQHRAQGPGPEPTLGPLTRLEGIKVR SEG xxxxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhhhhccccccccccccccccccccccccccccccccceeeeee MEM MMMMMMMMMMMMMMMMM SEQ TSLPRRAPGPARLSPRGPALSPGPHAAPGAALRRSRALPLTRLLSLSGPGRLGPFWAARS SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxx ..xxxx PRD eeccccccccccccccccccccccccccchhhhhhhcccccceeecccccccchhhhhhc MEM SEQ GAPAARCAPFP SEG xxxxxxxxx .. PRD ccccccccccc MEM (No Prosite data available for DKFZphutel_21dl5.2) (No Pfam data available for DKFZphutel_21dl5.2) DKFZphutel_22d2 group: signal transduction DKFZphutel_22d2 encodes a novel 580 amino acid putative GTP-binding protein related to the ras protein. Additionally, the putative protein contains an EF-hand for calcium-bmdmg. G-protems are involved in various signal transduction pathways, transferring the signal of a cellular receptor to an intracellular signal cascade. The new protein can find clinical application n modulating/blocking the response to a cellular receptor. similarity to GTP-binding proteins complete cDNA, complete eds, potential start at Bp 64, EST hits complete eds according to K08F11.5 and YAL048c Sequenced by BMFZ Locus: /map="17" Insert length: 3247 bp Poly A stretch at pos. 3230, no polyadenylation signal found 1 CTCCTGGTGA GAGGAGTCCA CTCCGTGCGT GCGGGCGGAG GCCGGCCCCC 51 GAGAGCCGCC GACATGAAGA AAGACGTGCG GATCCTGCTG GTGGGAGAAC 101 CTAGAGTTGG GAAGACATCA CTGATTATGT CTCTGGTCAG TGAAGAATTT 151 CCAGAAGAGG TTCCTCCCCG GGCAGAAGAA ATCACCATTC CAGCTGATGT 201 CACCCCAGAG AGAGTTCCAA CACACATTGT AGATTACTCA GAAGCAGAAC 251 AGAGTGATGA ACAACTTCAT CAAGAAATAT CTCAGGCTAA TGTCATCTGT 301 ATAGTGTATG CCGTTAACAA CAAGCATTCT ATTGATAAGG TAACAAGTCG 351 ATGGATTCCT CTCATAAATG AAAGAACAGA CAAAGACAGC AGGCTGCCTT 401 TAATATTGGT TGGGAACAAA TCTGATCTGG TGGAATATAG TAGTATGGAG 451 ACCATCCTTC CTATTATGAA CCAGTATACA GAAATAGAAA CCTGTGTGGA 501 GTGTTCAGCG AAAAACCTGA AGAACATATC AGAGCTCTTT TATTACGCAC 551 AGAAAGCTGT TCTTCATCCT ACAGGGCCCC TGTACTGCCC AGAGGAGAAG 601 GAGATGAAAC CAGCTTGTAT AAAAGCCCTT ACTCGTATAT TTAAAATATC 651 TGATCAAGAT AATGATGGTA CTCTCAATGA TGCTGAACTC AACTTCTTTC 701 AGAGGATTTG TTTCAACACT CCATTAGCTC CTCAAGCTCT GGAGGATGTC 751 AAGAATGTAG TCAGAAAACA TATAAGTGAT GGTGTGGCTG ACAGTGGGTT 801 GACCCTGAAA GGTTTTCTCT TTTTACACAC ACTTTTTATC CAGAGAGGGA 851 GACACGAAAC TACTTGGACT GTGCTTCGAC GATTTGGTTA TGATGATGAC 901 CTGGATTTGA CACCTGAATA TTTGTTCCCC CTGCTGAAAA TACCTCCTGA 951 TTGCACTACT GAATTAAATC ATCATGCATA TTTATTTCTC CAAAGCACCT 1001 TTGACAAGCA TGATTTGGAT AGAGACTGTG CTTTGTCACC TGATGAGCTT 1051 AAAGATTTAT TTAAAGTTTT CCCTTACATA CCTTGGGGGC CAGATGTGAA 1101 TAACACAGTT TGTACCAATG AAAGAGGCTG GATAACCTAC CAGGGATTCC 1151 TTTCCCAGTG GACGCTCACG ACTTATTTAG ATGTACAGCG GTGCCTGGAA 1201 TATTTGGGCT ATCTAGGCTA TTCAATATTG ACTGAGCAAG AGTCTCAAGC 1251 TTCAGCTGTT ACAGTGACAA GAGATAAAAA GATAGACCTG CAGAAAAAAC 1301 AAACTCAAAG AAATGTGTTC AGATGTAATG TAATTGGAGT GAAAAACTGT 1351 GGGAAAAGTG GAGTTCTTCA GGCTCTTCTT GGAAGAAACT TAATGAGGCA 1401 GAAGAAAATT CGTGAAGATC ATAAATCCTA CTATGCGATT AACACTGTTT 1451 ATGTATATGG ACAAGAGAAA TACTTGTTGT TGCATGATAT CTCAGAATCG 1501 GAATTTCTAA CTGAAGCTGA AATCATTTGT GATGTTGTAT GCCTGGTATA 1551 TGATGTCAGC AATCCCAAAT CCTTTGAATA CTGTGCCAGG ATTTTTAAGC 1601 AACACTTTAT GGACAGCAGA ATACCTTGCT TAATCGTAGC TGCAAAGTCA 1651 GACCTGCATG AAGTTAAACA AGAATACAGT ATTTCACCTA CTGATTTCTG 1701 CAGGAAACAC AAAATGCCTC CACCACAAGC CTTCACTTGC AATACTGCTG 1751 ATGCCCCCAG TAAGGATATC TTTGTTAAAT TGACAACAAT GGCCATGTAT 1801 CCGTAAGTAC TTGCTGTCTT CATTTTCATG TTGCATGGTT CATAACATTG 1851 CATGCCATTA TTAGCCATGA AGGGAATATC TTTGTCACAT AGGAATTGTT 1901 CAGCAACAGA AAGATACTTT GTAATGAGAA GGTACAAATT TGAGTAAATG 1951 CAAGTTTGGT TTGAATGCCA TAATAAAATG ATATAAACAG TGCTTCTGAC 2001 AATATCTGTA TATTTTTGAG CAGGCTGTAA CTATCTTAAT AGAATAGTAC 2051 AATAAAACAC AACCCCCCAC CCAGCATTAA AAAATAGTTT TACTGGAATA 2101 AAATGGGTTT GGCATCATGT TGTTTTATGC TTATAAAGCA TTTTCATATG 2151 AACAGAAAGT TTATATTTTT CTGTTTTTGA CCTTAGGTAT ATGAAGTTTT 2201 CTAAAATATT TTATTAATTT ATGTTGAAAT TGTGGGTATG CTTCAGTTAG 2251 GATATGTCTT TTTTAAGTGC TGTAAAGAGT AGTTGTAATT GGAATTTCTA 2301 CTGTATAAAT GTTTTACATT AAGTGTTACG AGCCACAAAT TTCATGTACA 2351 TTTATTATAT ATCTATACAT GCATATGCAC AAGCACATAA CTGTGGTCAT 2401 CTCTGTAGTT TACTAACTGC CTTAAAATTG CATGGTTCTT AATGGCATTC 2451 GCCTCAAGTA GTGTGTTTGT ATAAATTCTG TTTTGTAACA AAATAGTTTT 2501 TCAGGCAGTG CGTTTCTCAG GACTTTATAG CTTATTCTAC TTATTCTTAT 2551 GTTAGTCTCT AAATTATTTT TCTTCTTATG AAAACTACAG TGTAACACAG 2601 AGTAATAATC AAACATTGCT ATAAACCAAG AATGACATTT TTCAAAAAGG 2651 TGTTGATTTG TACAGATTTT TAAAGTCAGT TAACTTTACT GCTATTTTAT 2701 TACCTAATAC TTTTTTTAGA TGCAACAAAC CCTTGAATTT CTATTTGTAT 2751 TCGAAGACAA GTCATTCCTA TTATTATAGA ATAACCAAAA CCTTATTTAT 2801 GTTTTACCTT TGCTTTAAAA CTCTCATGTA TGTTATCTAC AGAGAGGATC 2851 ATTACAGAGA CAGACTCTCC CGAGACATGG GCCACACTGA TAGAATAGAG 2901 AATTTGAGAA AAATCTGGGT CTTTCTAAAA ACTGCTTTGT AAGTTACTTT 2951 TTCTTTATGA CTTCTGTGGG ATTTTGTTGA TATTTTCTTA GAGAATGACC 3001 AAATCTCCTT TCTTGCCATA ATTAACATTT AGTAATTATG TAGAAACGCA 3051 CTGCTTGGTC AGGCTTCCTG CCTAGCTATA TATTACGTTG TCTTCCTTAC 3101 TACATAAATG TACTTCTTTA ATCTTGTGAT TACAGTAACT GCAAGTGTGT 3151 TTTTACATCT GCATTTTTAA AACATTTTAC TGTAATTCTG TTGTGTGTGT 3201 GTGTGTTATA TGATAAATGT ACATACATGG AAAAAAAAAA AAAAAAA BLAST Results Entry AC004527 from database EMBL: *** SEQUENCING IN PROGRESS *** NFl-related locus, Direct Submission; HTGS phase 1, 10 unordered pieces. Score = 1899, P = l.le-78, identities = 387/396 Entry HS148355 from database EMBL: human STS SHGC-31220. Score = 1826, P = 7.5e-78, identities 388/406 Medline entries No Medline entry Peptide information for frame 1 ORF from 64 bp to 1803 bp; peptide length: 580 Category: similarity to known protein 1 MKKDVRILLV GEPRVGKTSL IMΞLVSEEFP EEVPPRAEEI TIPADVTPER 51 VPTHIVDYSE AEQSDEQLHQ EISQANVICI VYAVNNKHSI DKVTSRWIPL 101 INERTDKDSR LPLILVGNKS DLVEYSSMET ILPIMNQYTE IETCVECSAK 151 NLKNISELFY YAQKAVLHPT GPLYCPEEKE MKPACIKALT RIFKISDQDN 201 DGTLNDAELN FFQRICFNTP LAPQALEDVK NVVRKHISDG VADSGLTLKG 251 FLFLHTLFIQ RGRHETTWTV LRRFGYDDDL DLTPEYLFPL LKIPPDCTTE 301 LNHHAYLFLQ STFDKHDLDR DCALSPDELK DLFKVFPYIP WGPDVNNTVC 351 TNERGWITYQ GFLSQWTLTT YLDVQRCLEY LGYLGYSILT EQESQASAVT 401 VTRDKKIDLQ KKQTQRNVFR CNVIGVKNCG KSGVLQALLG RNLMRQKKIR 451 EDHKSYYAIN TVYVYGQEKY LLLHDISESE FLTEAEIICD VVCLVYDVSN 501 PKSFEYCARI FKQHFMDSRI PCLIVAAKSD LHEVKQEYSI SPTDFCRKHK 551 MPPPQAFTCN TADAPΞKDIF VKLTTMAMYP BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphutel_22d2, frame 1 TREMBL :CEUK08F11_3 gene: "K08F11.5"; Caenorhabditis elegans cosmid K08F11., N = 1, Score = 1357, P = l.le-138 TREMBL :SPCC320_4 gene: "SPCC320.04c"; product: "hypothetical protein" S. pombe chromosome III cosmid c320., N = 1, Score = 889, P = 4.4e-89 TREMBL :CEUC47C12_3 gene: "C47C12.4"; Caenorhabditis elegans cosmid C47C12., N = 2, Score = 408, P = 5.6e-74 PIR:S51971 probable membrane protein YAL048c - yeast (Saccharomyces cerevisiae), N = 1, Score = 677, P = 1.3e-66 >TREMBL:CEUK08F11_3 gene: "K08F11.5"; Caenorhabditis elegans cosmid K08F11. Length = 625 HSPs: Score = 1357 (203.6 bits), Expect = l.le-138, P = l.le-138 Identities = 263/582 (45%), Positives = 380/582 (65%) Query: 4 DVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPADVTPERVPTHIVDYSEAEQ 63 DVRI+L+G+ GKTSL+MSL+ +E+ + VP R + + IPADVTPE V T IVD S E+ Sbjct: 9 DVRIVLIGDEGCGKTSLVMΞLLEDEWVDAVPRRLDRVLIPADVTPENVTTSIVDLSIKEE 68 Query: 64 SDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLINERTDKDSRLPLILVGNKSDLV 123 + + El QANVIC+VY+V ++ ++D + ++W+PLI + + P+ILVGNKSD Sbjct: 69 DENWIVΞEIRQANVICVVYSVTDESTVDGIQTKWLPLIRQSFGEYHETPVILVGNKSDGT 128 Query: 124 EYSSMETILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMKP 183 ++ + ILPIM TE+ETCVECSA+ +KN+SE+FYYAQKAV++PT PLY + K++ Sbjct: 129 A-NNTDKILPIMEANTEVETCVECSARTMKNVSEIFYYAQKAVIYPTRPLYDADTKQLTD 187 Query: 184 ACIKALTRIFKISDQDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHISDGVAD 243 KAL R+FKI D+DNDG L+D ELN FQ++CF PL ALEDVK V DGVA+ Sbjct: 188 RARKALIRVFKICDRDNDGYLSDTELNDFQKLCFGIPLTSTALEDVKRAVSDGCPDGVAN 2 47 Query: 244 SGLTLKGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTELNH 303 L L GFL+LH LFI+RGRHETTW VLR+FGY+ L L+ +YL+P + IP C+TEL+ Sbjct: 248 DSLMLAGFLYLHLLFIERGRHETTWAVLRKFGYETSLKLSEDYLYPRITIPVGCSTELΞP 307 Query: 304 HAYLFLQSTFDKHDLDRDCALSPDELKDLFKVFPYIPWGPDVNNTVCTNERGWITYQGFL 363 F+ + F+K+D D+D LSP EL++LF V P D + TN+RGW+TY G++ Sbjct: 308 EGVQFVSALFEKYDEDKDGCLSPSELQNLFSVCPVPVITKDNILALETNQRGWLTYNGYM 367 Query: 364 SQWTLTTYLDVQRCLEYLGYLGYSILTEQESQAS AVTVTRDKKIDLQKKQTQRNVF 419 + W +TT +++ + E L YLG+ + +A ++ VTR++K DL+ T R VF Sbjct: 368 AYWNMTTLINLTQTFEQLAYLGFPVGRSGPGRAGNTLDSIRVTRERKKDLENHGTDRKVF 427 Query: 420 RCNVIGVKNCGKSGVLQALLGRNLMRQKKIREDHKSYYAINTVYVYGQEKYLLLHDI 476 +C V+G K+ GK+ +Q+L GR + +1 H S + IN V V + KYLLL ++ Sbjct: 428 QCLVVGAKDAGKTVFMQSLAGRGMADVAQIGRRH-SPFVINRVRVKEESKYLLLREVDVL 486 Query: 477 SESEFLTEAEIICDVVCLVYDVSNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQ 536 S + L E DVV +YD+SNP SF +CA +++++F ++ PC+++A K + EV Q Sbjct: 487 SPQDALGSGETSADVVAFLYDISNPDSFAFCATVYQKYFYRTKTPCVMIATKVEREEVDQ 546 Query: 537 EYΞISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYP 580 + + P +FCR+ ++P P F+ S IF +L MA+YP Sbjct: 547 RWEVPPEEFCRQFELPKPIKFSTGNIGQSSSPIFEQLAMMAVYP 590 Pedant information for DKFZphutel_22d2, frame 1 Report for DKFZphutel_22d2.1 [LENGTH] 580 [MW] 66541.61 [pi] 5.56 [HOMOL] TREMBL:CEUK08F11_3 gene: "K08F11.5"; Caenorhabditis elegans cosmid K08F11. le- 149 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YAL048c] 5e-81 [FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YKR055w] 3e-ll [FUNCAT] 03.99 other cell growth, cell division and dna synthesis activities [S. cerevisiae, YNL098c] 8e-09 [FUNCAT] 10.04.07 g-proteins [S. cerevisiae, YNL098c] 8e-09 [FUNCAT] 03.10 sporulation and germination [S. cerevisiae, YNL098c] 8e-09 [FUNCAT] 11.01 stress response [S. cerevisiae, YNL098c] 8e-09 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YNL098c] 8e-09 [FUNCAT] 01.03.13 regulation of nucleotide metabolism [S. cerevisiae, YNL098c] 8e-09 [ FUNCAT ] 01.05.04 regulation of carbohydrate utilization [S. cerevisiae, YNL098c] 8e-09 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YORlOlw] 4e-08 [FUNCAT] 11.10 cell death [S. cerevisiae, YORlOlw] 4e-08 [FUNCAT] 10.02.07 g-proteins [S. cerevisiae, YPR165w] 7e-08 [FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YPR165w] 7e-08 [FUNCAT] 30.08 organization of golgi [S. cerevisiae, YPR165w] 7e-08 [FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YFL005w] 9e-08 [FUNCAT] 30.09 organization of intracellular transport vesicles [S. cerevisiae, YFL005w] 9e -08 [FUNCAT] 30.02 organization of plasma membrane [S. cerevisiae, YFL005w] 9e-06 [FUNCAT] 08.13 vacuolar transport [S. cerevisiae, YNL093w] le-07 [FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YNL093w] le-07 [FUNCAT] 08.19 cellular import [S. cerevisiae, YNL093w] le-07 [FUNCAT] 10.05.07 g-proteins [S. cerevisiae, YLR229C] 8e-07 [FUNCAT] 03.07 pheromone response, mating-type determination, sex-specific proteins [S. cerevisiae, YLR229c] 8e-07 [FUNCAT] 10.99 other signal-transduction activities [S. cerevisiae, YCR027c] 3e-06 [FUNCAT] 09.09 biogenesis of intracellular transport vesicles [S. cerevisiae, YGL210w] 9e-04 [BLOCKS] BL00410A Dynamin family proteins [SCOP] dlplk 3.25.1.3.1 cH-p21 Ras protein [human (Homo sapiens) 2e-42 [SCOP] dlguaa_ 3.25.1.3.10 RaplA [Human (Homo sapiens) 5e-59 [PIRKW] transmembrane protein le-79 [PIRKW] membrane trafficking 2e-06 [PIRKW] acetylated am o end 3e-09 [PIRKW] prenylated cysteine 3e-09 [PIRKW] signal transduction le-07 [PIRKW] transforming protein 3e-09 [PIRKW] immediate-early protein 8e-06 [PIRKW] alternative splicing 4e-08 [PIRKW] P-loop le-10 [PIRKW] lipoprotein 7e-10 [PIRKW] proto-oncogene 3e-09 [PIRKW] methylated carboxyl end 3e-09 [PIRKW] membrane protein 3e-09 [PIRKW] GTP binding le-10 [PIRKW] thiolester bond 7e-10 [SUPFAM] ras transforming protein le-10 [PROSITE] ATP_GTP_A 2 [PROSITE] MYRISTYL 3 [PROSITE] EF_HAND 1 [PROSITE] CAMP_PHOSPHO_SITE 1 [PROSITE] CK2_PHOSPHO_SITE 14 [PROSITE] TYR_PHOSPHO_SITE 4 [PROSITE] PKC_PHOSPHO_SITE 5 [PROSITE] ASN_GLYCOSYLATION 3 [PFAM] Ras family (contains ATP/GTP binding P-loop) [KW] Irregular [KW] 3D SEQ MKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPADVTPERVPTHIVDYSE ljai- ...EEEEEEEETTTTCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEETTEEEEEEEEECCC SEQ AEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLINERTDKDSRLPLILVGNKS ljai- CGGGHHHHHHHHHHTTEEEEEEETTTHHHHHHH-HHHHHHHHHHHCTTT-TCEEEEEETT SEQ DLVEYSSMETILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKE ljai- TTTTTTTTHHHHHHHHHHHCCCE-EECTTTTTTTHHHHHH SEQ MKPACIKALTRIFKISDQDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHIΞDG SEQ VADSGLTLKGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTE SEQ LNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFKVFPYIPWGPDVNNTVCTNERGWITYQ ljai- SEQ GFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAVTVTRDKKIDLQKKQTQRNVFR SEQ CNVIGVKNCGKSGVLQALLGRNLMRQKKIREDHKSYYAINTVYVYGQEKYLLLHDISESE SEQ FLTEAEIICDVVCLVYDVSNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEYSI SEQ SPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYP Prosite for DKFZphutel 22d2.1 PS00001 118->122 ASN_GLYCOSYLATION PDOC00001 PS00001 154->158 ASN_GLYCOSYLATION PDOC00001 PS00001 346->350 AΞN_GLYCOSYLATION PDOC00001 PS00004 411->415 CAMP_PHOSPHO_SITE PDOC00004 PS00005 94->97 PKC_PHOSPHO_SITE PDOC00005 PS00005 105->108 PKC PHOSPHO SITE PDOC00005 PS00005 148->151 PKC PHOSPHO_ SITE PDOC00005 PS00005 247->250 PKC PHOSPHO^ " SITE PDOC00005 PS00005 414->417 PKC PHOSPHO " " SITE PDOC00005 PS00006 59->63 CK2 PHOSPHO " " SITE PDOC00006 PS00006 105->109 CK2 PHOSPHO " " SITE PDOC00006 PS00006 126->130 CK2 PHOSPHO "" " SITE PDOC00006 PS00006 139->143 CK2 PHOSPHO_ " SITE PDOC00006 PS00006 143->147 CK2 PHOSPHO " " SITE PDOC00006 PS00006 196->200 CK2 PHOSPHO " " SITE PDOC00006 PS00006 203->207 CK2 PHOSPHO " SITE PDOC00006 PS00006 311->315 CK2 PHOSPHO " " SITE PDOC00006 PS00006 325->329 CK2 PHOSPHO " " SITE PDOC00006 PS00006 370->374 CK2 PHOSPHO " " SITE PDOC00006 PS00006 390->394 CK2 PHOSPHO " " SITE PDOC00006 PS00006 477->481 CK2 PHOSPHO " SITE PDOC00006 PS00006 483->487 CK2 PHOSPHO " " SITE PDOC00006 PS00006 541->545 CK2 PHOSPHO ~ " SITE PDOC00006 PS00007 153->161 TYR PHOSPHO_ " SITE PDOC00007 PS00007 376->384 TYR PHOSPHO " SITE PDOC00007 PS00007 153->162 TYR PHOSPHO " SITE PDOC00007 PS00007 448->457 TYR PHOSPHO " " SITE PDOC00007 PS00008 240->246 MYRISTYL PDOC00008 PS00008 425->431 MYRISTYL PDOC00008 PS00008 433->439 MYRISTYL PDOC00008 PS00017 11->19 ATP GTP A PDOC00017 PS00017 425->433 ATP GTP A PDOC00017 PΞ00018 197->210 EF HAND PDOC00018 Pfam for DKFZphutel 22d2.1 HMM_NAME Ras family (contains ATP/GTP binding P-loop) HMM *KLVLIGDSGVGKSCLLIRFTQNeFnEeYIPTIGvDFYtKTIEIDGKtIK ++L+G+ VGK++L ++ EF+EE +P ++ T ++ +++ Query 6 RILLVGEPRVGKTSLIMSLVSEEFPEE-VPPR-AEEITIPADVTPERVP 52 HMM LQIWDTAGQERYRsMRPMYYRGAMGFMLVYDITNRqSFENIr.NWweEIr I D E+ + + + +A+++ +VY+++N+ S ++++ +W++ 1+ Query 53 THIVDYSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLIN 102 HMM RHCDrDENVPIMLVGNKCDLEDQRQVStEEGQeFAREWGAIPFMETSAKT + D+D+ P +LVGNK+DL + ++T + +E+SAK+ Query 103 ERTDKDSRLPLILVGNKSDLVEYΞSMETILPIMNQYTEI-ETCVECSAKN 151 HMM NiNVEEAFMEIvRellqrMqeqNqteNimdQpsrnrkrCCCIM* N+ E F+ + +++L + +++ +++++ + C+ Query 152 LKNISELFYYAQKAVLHPT GPLYCPEEKEMK-PACI— 186 DKFZphutel_22el2 group: signal transduction DKFZphutel_22el2 encodes a novel 92 amino acid protein, with similarity to yeast, C. elegans, Drosophila and mammalian proteins. The Drosophila cm and mammalian cornicon proteins are part of a signal transduction pathway involving hte EGF-receptor. The new protein can find application in modulating the cornichon modulated signal transduction way and also the EGF receptor signaling processes. strong similarity to S. cerevisiae YGL054c and cornichon complete cDNA, complete eds, EST hits cornicon is requiered for signal transduction in the EGF-receptor signal processing Sequenced by BMFZ Locus: unknown Insert length: 519 bp Poly A stretch at pos. 499, no polyadenylation signal found 1 GTCGGGGCAT CCGAGCGGGT TTGACGGAAG GAGCGGCGGC GACGGAGGAG 51 GAGGATGGAG GCGGTGGTGT TCGTCTTCTC TCTCCTCGAT TGTTGCGCGC 101 TCATCTTCCT CTCGGTCTAC TTCATAATTA CATTGTCTGA TTTAGAATGT 151 GATTACATTA ATGCTAGATC ATGTTGCTCA AAATTAAACA AGTGGGTAAT 201 TCCAGAATTG ATTGGCCATA CCATTGTCAC TGTATTACTG CTCATGTCAT 251 TGCACTGGTT CATCTTCCTT CTCAACTTAC CTGTTGCCAC TTGGAATATA 301 TATCGTATGA TCTTAGCTTT GATAAATGAC TGAAGCTGGA GAAGCCGTGG 351 TTGAAGTCAG CCTACACTAC AGTGCACAGT TGAGGAGCCA GAGACTTCTT 401 AAATCATCCT TAGAACCGTG ACCATAGCAG TATATATTTT CCTCTTGGAA 451 CAAAAAACTA TTTTTGCTGT ATTTTTACCA TATAAAGTAT TTAAAAAACA 501 TGAAAAAAAA AAAAAAAAA BLAST Results No BLAST result Medline entries 95300228: cornichon and the EGF receptor signaling process are necessary for both anterior-posterior and dorsal-ventral pattern formation in Drosophila. Peptide information for frame 1 ORF from 55 bp to 330 bp; peptide length: 92 Category: strong similarity to known protein 1 MEAVVFVFSL LDCCALIFLS VYFIITLSDL ECDYINARSC CSKLNKWVIP 51 ELIGHTIVTV LLLMSLHWFI FLLNLPVATW NIYRMILALI ND BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphutel_22el2, frame 1 PIR:S64058 probable membrane protein YGL054c - yeast (Saccharomyces cerevisiae), N = 2, Score = 185, p = 5.7e-17 TREMBL :SPAC2C4_5 gene: "SPAC2C4.05"; product: "cornichon homolog"; S. pombe chromosome I cosmid c2C4. , N = 1, Score = 163, P = 3.7e-12 PIR:S46084 probable membrane protein YBR210w - yeast (Saccharomyces cerevisiae), N = 1, Score = 162, P = 4.8e-12 TREMBL:AF104398_1 product: "cornichon"; Homo sapiens cornichon mRNA, complete eds., N = 1, Score = 141, P = 8e-10 SWISSPROT :CNI_DROVI CORNICHON PROTEIN., N = 1, Score = 139, P = 1.3e-09 >PIR:S64058 probable membrane protein YGL054c - yeast (Saccharomyces cerevisiae) Length = 138 HSPs: Score = 185 (27.8 bits), Expect = 5.7e-17, Sum P(2) = 5.7e-17 Identities = 35/85 (41%), Positives = 56/85 (65%) Query: 1 MEAVVFVFSLLDCCALIFLSVYFIITLSDLECDYINARSCCSKLNKWVIPELIGHTIVTV 60 M A +F+ +++ C +F V+F I +DLE DYIN CSK+NK + PE H +++ Sbjct: 1 MGAWLFILAVVVNCINLFGQVHFTILYADLEADYINPIELCSKVNKLITPEAALHGALSL 60 Query: 61 LLLMSLHWFIFLLNLPVATWNIYRM 85 L L++ +WF+FLLNLPV +N+ ++ Sbjct: 61 LFLLNGYWFVFLLNLPVLAYNLNKI 85 Score = 37 (5.6 bits), Expect = 5.7e-17, Sum P(2) = 5.7e-17 Identities = 7/9 (77%), Positives = 9/9 (100%) Query: 82 IYRMILALI 90 +YRMI+ALI Sbjct: 123 LYRMIMALI 131 Pedant information for DKFZphutel_22el2, frame 1 Report for DKFZphutel_22el2.1 [LENGTH] 92 [MW] 10614.98 [pi] 5.04 [HOMOL] PIR:S64058 probable membrane protein YGL054c - yeast (Saccharomyces cerevisiae) 5e-14 [FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YGL054c] 2e-15 [PIRKW] transmembrane protein 2e-ll [PROSITE] CK2_PHOSPHO_SITE 3 [KW] SIGNAL_PEPTIDE 33 [KW] TRANSMEMBRANE 2 SEQ MEAVVFVFSLLDCCALIFLSVYFIITLSDLECDYINARSCCSKLNKWVIPELIGHTIVTV PRD ccchhhhhhhhhhhhhhhhhhhheeeccccccccccccccccccceeehhhhhhhhhhhh MEM MMMMMMMMMM SEQ LLLMSLHWFIFLLNLPVATWNIYRMILALIND PRD hhhhhhhheeecccccchhhhhhhhhhhhccc MEM MMMMMMMMMMMMMMMMMMM .. MMMMMMM .... Prosite for DKFZphutel 22el2.1 PS00006 9->13 CK2 PHOSPHO SITE PDOC00006 PS00006 26->30 CK2 PHOSPHO SITE PDOC00006 PS00006 28->32 CK2 PHOSPHO SITE PDOC00006 (No Pfam data available for DKFZphutel_22el2.1) DKFZphutel_22n2 group: uterus derived DKFZphutel_22n2 encodes a novel 304 amino acid protein without similarity to known proteins. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of uterus-specific genes . unknown complete cDNA, complete eds, EST hits Sequenced by BMFZ Locus: /map="553.3 cR from top of Chrll linkage group" Insert length: 1556 bp Poly A stretch at pos. 1534, no polyadenylation signal found 1 ACAACAGGCT GGTTGCTTGG CGTGGAATCC TAAAGTGGCC TGGCTTTGAG 51 ACTGGAGTGA GACCCCAGCC CTAGGCTGGG GTTCTTTCCA TTATAGAGGA 101 GACGGATTCA GAAGGGCTAC AGACCAAGGT TGTTGAAAAC CAGACATATG 151 ATGAGCGTCT AGAGATTAAC GACTCCGAAG AGGTTGCAAG TATTTATACT 201 CCAACCCCAA GACACCAAGG ACTTCCTCGT TCTGCCCATC TTCCTAACAA 251 GGCTATGGCT GATAACAGCA GTGATGAGTG TGAAGAGGAA AATAACAAGG 301 AGAAGAAGAA GACCTCACAG TTGACACCTC AACGGGGCTT TAGTGAAAAT 351 GAGGATGACG ATGATGATGA TGATGATTCA TCTGAAACTG ATTCTGATTC 401 TGATGATGAT GATGAAGAGC ATGGAGCCCC TCTGGAAGGG GCCTATGACC 451 CTGCAGACTA TGAGCATTTG CCAGTTTCTG CTGAAATTAA GGAACTCTTC 501 CAGTACATCA GTAGGTACAC ACCTCAGTTG ATTGACCTGG ACCACAAACT 551 GAAGCCTTTC ATTCCTGATT TTATCCCAGC TGTCGGGGAT ATTGATGCAT 601 TCTTAAAGGT CCCACGTCCT GATGGAAAGC CTGACAACCT TGGCCTATTG 651 GTATTGGATG AACCTTCTAC AAAGCAGTCA GACCCTACGG TGCTCTCACT 701 CTGGTTAACA GAGAATTCTA AGCAGCACAA CATCACACAA CATATGAAAG 751 TAAAAAGCCT AGAAGATGCA GAAAAGAATC CCAAAGCCAT TGACACGTGG 801 ATTGAGAGCA TCTCTGAATT ACACCGTTCT AAGCCCCCTG CGACTGTGCA 851 CTACACCAGG CCCATGCCCG ACATTGACAC GCTGATGCAG GAATGGTCCC 901 CGGAGTTTGA AGAGCTTTTG GGCAAGGTAA GCCTGCCCAC GGCAGAGATT 951 GATTGCAGCC TGGCAGAGTA CATTGACATG ATCTGTGCCA TTCTAGACAT 1001 CCCTGTCTAC AAGAGTCGGA TCCAGTCCCT CCATCTGCTC TTTTCCCTCT 1051 ACTCAGAATT CAAGAACTCA CAGCATTTTA AAGCTCTCGC TGAAGGCAAG 1101 AAAGCATTCA CTCCTTCATC CAATTCCACC TCCCAAGCTG GAGACATGGA 1151 GACATTAACC TTCAGCTGAG ACACTTCCCA AGCTGCTGTT TCAAGGCTGA 1201 GCTGGCCCCT CTGCCCCAGC TGAGATGGAC AGATCGTTGT CAGCTACTTG 1251 ATGTCCTTGC CCATGCCACA GCTTGGCTCA GGGGCAGTGC ATGTCCTGCT 1301 GCCCTCTCTG CCAGAGGGCA CAGAACATGT TTGTTTAATG AACCTGCCTG 1351 CCTCAGATTG CTGTCCCCGG GGAGTTAATG CATCTACACC ACTGTGGGGA 1401 TTTGAGTTAT AAGAATTGGA ATTTCTGAGA TCCCATGGAG GTTAGATTGG 1451 GAGGAAAGCT TAAAAGATGT CCTTTTTGTG AGAGGGATGG AATTGTTTTC 1501 TTTCATTCGT AAAGTTAGTG AGTAAAGATT TTATAAATCA AAAAAAAAAA 1551 AAAAAA BLAST Results Entry HS188252 from database EMBL: human STS WI-12265. Score = 2554, P = 4.1e-109, identities 556/587 Medline entries No Medline entry Peptide information for frame 3 ORF from 255 bp to 1166 bp; peptide length: 304 Category: putative protein 1 MADNSSDECE EENNKEKKKT SQLTPQRGFS ENEDDDDDDD DSSETDSDSD 51 DDDEEHGAPL EGAYDPADYE HLPVSAEIKE LFQYISRYTP QLIDLDHKLK 101 PFIPDFIPAV GDIDAFLKVP RPDGKPDNLG LLVLDEPSTK QSDPTVLSLW 151 LTENSKQHNI TQHMKVKSLE DAEKNPKAID TWIESISELH RSKPPATVHY 201 TRPMPDIDTL MQEWSPEFEE LLGKVSLPTA EIDCΞLAEYI DMICAILDIP 251 VYKSRIQSLH LLFSLYSEFK NSQHFKALAE GKKAFTPSSN STSQAGDMET 301 LTFS BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphutel_22n2, frame 3 PIR:S38149 SIS2 protein - yeast (Saccharomyces cerevisiae), N = 1, Score = 132, P = le-05 >PIR:S38149 SIS2 protein - yeast (Saccharomyces cerevisiae) Length = 562 HSPs: Score = 132 (19.8 bits), Expect = 1.0e-05, P = 1.0e-05 Identities = 24/63 (38%), Positives = 35/63 (55%) Query: 3 DNSSDECEEENNKEKKKTSQLTPQRGFSENEDDDDDDDDSSETDΞDSDDDDEEHGAPLEG 62 + DE EEE++ E++ T +++DDDDDDDD + D D DDD++E A G Sbjct: 497 EEDDDEDEEEDDDEEEDTEDKNENNNDDDDDDDDDDDDDDDDDDDDDDDDEDEDEAETPG 556 Query: 63 AYD 65 D Sbjct: 557 IID 559 Score = 122 (18.3 bits). Expect = 1.4e-04, P = 1.4e-04 Identities = 20/52 (38%), Positives = 33/52 (63%) Query: 4 NSSDECEEENNKEKKKTSQLTPQRGFSENEDDDDDDDDSSETDSDSDDDDEE 55 N+ +E ++E+ +E + T + + N+DDDDDDDD + D D DDDD++ Sbjct: 494 NNEEEDDDEDEEEDDDEEEDTEDKNENNNDDDDDDDDDDDDDDDDDDDDDDD 545 Pedant information for DKFZphutel_22n2, frame 3 Report for DKFZphutel_22n2.3 [LENGTH] 304 [MW] 34285.85 [pi] 4.37 [PROSITE] AMIDATION 1 [PROSITE] CAMP_PHOSPHO_SITE 2 [PROSITE] CK2_PHOSPHO_SITE 10 [PROSITE] PKC_PHOSPHO_SITE 1 [PROSITE] ASN_GLYCOSYLATION 3 [KW] All_Alpha [KW] LOW_COMPLEXITY 11.84 % SEQ MADNSSDECEEENNKEKKKTSQLTPQRGFSENEDDDDDDDDSSETDSDSDDDDEEHGAPL SEG xxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxx PRD ccccccchhhhhhchhhhhhcccccccccccccccccccccccccccccccccccccccc SEQ EGAYDPADYEHLPVΞAEIKELFQYISRYTPQLIDLDHKLKPFIPDFIPAVGDIDAFLKVP SEG PRD ccccccccccccchhhhhhhhhhhhhhhccccccccccccccccccccccccccceeecc SEQ RPDGKPDNLGLLVLDEPSTKQSDPTVLSLWLTENSKQHNITQHMKVKSLEDAEKNPKAID SEG PRD ccccccccceeeeecccccccccccchhhhhhccccccccccccchhhhhhhhcccccch SEQ TWIESISELHRSKPPATVHYTRPMPDIDTLMQEWSPEFEELLGKVSLPTAEIDCSLAEYI SEG PRD hhhhhhhhhhcccccceeeeecccccchhhhhhcccchhhhhccccccccccchhhhhhh SEQ DMICAILDIPVYKSRIQΞLHLLFSLYSEFKNSQHFKALAEGKKAFTPSSNSTSQAGDMET SEG PRD hhhhhhhcccchhhhhhhhhhhhhhhhhhhcchhhhhhhhcccccccccccccccccccc SEQ LTFS SEG .... PRD cccc Prosite for DKFZphutel_22n2.3 PS00001 4->8 ASN_GLYCOSYLATION PDOC00001 PS00001 159->163 ASN_GLYCOSYLATION PDOC00001 PS00001 290->294 ASN_GLYCOSYLATION PDOC00001 PS00004 17->21 CAMP_PHOSPHO_SITE PDOC00004 PS00004 18->22 CAMP_PHOSPHO_SITE PDOC00004 PS00005 138->141 PKC_PHOSPHO_SITE PDOC00005 PS00006 5->9 CK2_PHOSPHO_SITE PDOC00006 PS00006 30->34 CK2_PHOSPHO_SITE PDOC00006 PS00006 43->47 CK2_PHOSPHO_SITE PDOC00006 PS00006 45->49 CK2_PHOSPHO_SITE PDOC00006 PS00006 47->51 CK2_PHOSPHO_SITE PDOC00006 PS00006 49->53 CK2_PHOSPHO_SITE PDOC00006 PS00006 168->172 CK2_PHOSPHO_SITE PDOC00006 PS00006 181->185 CK2_PHOSPHO_SITE PDOC00006 PS00006 185->189 CK2_PHOSPHO_SITE PDOC00006 PS00006 235->239 CK2_PHOSPHO_SITE PDOC00006 PS00009 280->284 AMIDATION PDOC00009 (No Pfam data available for DKFZphutel_22n2.3) DKFZphutel_22o2 group: uterus derived DKFZphutel_22o2 encodes a novel 537 amino acid protein without similarity to known proteins. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of uterus-specific genes . similarity to S. pombe SPBC3E7.03c complete cDNA, complete eds, EST hits Sequenced by BMFZ Locus: map="llpl5.5" Insert length: 2714 bp Poly A stretch at pos. 2695, polyadenylation signal at pos. 2677 1 GCAGGGCACG GTGGGGGCTG AGATCGTTTC CTGTTGGAAC TTCTGGCCCA 51 AGAAGCGCGG GTCACAAGGA GAGGGGTCAG TTCGGTTCAG AGCGACTCAG 101 CCCCTCGACT CGGGTCTTAA AACCTCCGAG CCGCCAGTTC TGCCTCAGGC 151 CGCGCCCCCT TAAAGCGCCA CCAGACGCTG CGCCCCGTTA AAGCGCCACC 201 AGACGCCGCG CCCCGTCCCG GCCTCCCCCG CGCGCTGGCG CGGGGCTTTC 251 TGGGCCAGGG CGGGGCCGGC GAACTGCGGC CCGGAACGGC TGAGGAAGGG 301 CCCGTCCCGC CTTCCCCGGC GCGCCATGGA GCCCCGGGCG GTTGCAGAAG 351 CCGTGGAGAC GGGTGAGGAG GATGTGATTA TGGAAGCTCT GCGGTCATAC 401 AACCAGGAGC ACTCCCAGAG CTTCACGTTT GATGATGCCC AACAGGAGGA 451 CCGGAAGAGA CTGGCGGAGC TGCTGGTCTC CGTCCTGGAA CAGGGCTTGC 501 CACCCTCCCA CCGTGTCATC TGGCTGCAGA GTGTCCGAAT CCTGTCCCGG 551 GACCGCAACT GCCTGGACCC GTTCACCAGC CGCCAGAGCC TGCAGGCACT 601 AGCCTGCTAT GCTGACATCT CTGTCTCTGA GGGGTCCGTC CCAGAGTCCG 651 CAGACATGGA TGTTGTACTG GAGTCCCTCA AGTGCCTGTG CAACCTCGTG 701 CTCAGCAGCC CTGTGGCACA GATGCTGGCA GCAGAGGCCC GCCTAGTGGT 751 GAAGCTCACA GAGCGTGTGG GGCTGTACCG TGAGAGGAGC TTCCCCCACG 801 ATGTCCAGTT CTTTGACTTG CGGCTCCTCT TCCTGCTAAC GGCACTCCGC 851 ACCGATGTGC GCCAGCAGCT GTTTCAGGAG CTGAAAGGAG TGCGCCTGCT 901 AACTGACACA CTGGAGCTGA CGCTGGGGGT GACTCCTGAA GGGAACCCCC 951 CACCCACGCT CCTTCCTTCC CAAGAGACTG AGCGGGCCAT GGAGATCCTC 1001 AAAGTGCTCT TCAACATCAC CCTGGACTCC ATCAAGGGGG AGGTGGACGA 1051 GGAAGACGCT GCCCTTTACC GACACCTGGG GACCCTTCTC CGGCACTGTG 1101 TGATGATCGC TACTGCTGGA GACCGCACAG AGGAGTTCCA CGGCCACGCA 1151 GTGAACCTCC TGGGGAACTT GCCCCTCAAG TGTCTGGATG TTCTCCTCAC 1201 CCTGGAGCCA CATGGAGACT CCACGGAGTT CATGGGAGTG AATATGGATG 1251 TGATTCGTGC CCTCCTCATC TTCCTAGAGA AGCGTTTGCA CAAGACACAC 1301 AGGCTGAAGG AGAGTGTAGC TCCCGTGCTG AGCGTGCTGA CTGAATGTGC 1351 CCGGATGCAC CGCCCAGCCA GGAAGTTCCT GAAGGCCCAG GGATGGCCAC 1401 CTCCCCAGGT GCTGCCCCCT CTGCGGGATG TGAGGACACG GCCTGAGGTT 1451 GGGGAGATGC TGCGGAACAA GCTTGTCCGC CTCATGACAC ACCTGGACAC 1501 AGATGTGAAG AGGGTGGCTG CCGAGTTCTT GTTTGTCCTG TGCTCTGAGA 1551 GTGTGCCCCG ATTCATCAAG TACACAGGCT ATGGGAATGC TGCTGGCCTT 1601 CTGGCTGCCA GGGGCCTCAT GGCAGGAGGC CGGCCCGAGG GCCAGTACTC 1651 AGAGGATGAG GACACAGACA CAGATGAGTA CAAGGAAGCC AAAGCCAGCA 1701 TAAACCCTGT GACCGGGAGG GTGGAGGAGA AGCCGCCTAA CCCTATGGAG 1751 GGCATGACAG AGGAGCAGAA GGAGCACGAG GCCATGAAGC TGGTGACCAT 1801 GTTTGACAAG CTCTCCAGGA ACAGAGTCAT CCAGCCAATG GGGATGAGTC 1851 CCCGGGGTCA TCTTACGTCC CTGCAGGATG CCATGTGCGA GACTATGGAG 1901 CAGCAGCTCT CCTCGGACCC TGACTCGGAC CCTGACTGAG GATGGCAGCT 1951 CTTCTGCTCC CCCATCAGGA CTGGTGCTGC TTCCAGAGAC TTCCTTGGGG 2001 TTGCAACCTG GGGAAGCCAC ATCCCACTGG ATCCACACCC GCCCCCACTT 2051 CTCCATCTTA GAAACCCCTT CTCTTGACTC CCGTTCTGTT CATGATTTGC 2101 CTCTGGTCCA GTTTCTCATC TCTGGACTGC AACGGTCTTC TTGTGCTAGA 2151 ACTCAGGCTC AGCCTCGAAT TCCACAGACG AAGTACTTTC TTTTGTCTGC 2201 GCCAAGAGGA ATGTGTTCAG AAGCTGCTGC CTGAGGGCAG GGCCTACCTG 2251 GGCACACAGA AGAGCATATG GGAGGGCAGG GGTTTGGGTG TGGGTGCACA 2301 CAAAGCAAGC ACCATCTGGG ATTGGCACAC TGGCAGAGCC AGTGTGTTGG 2351 GGTATGTGCT GCACTTCCCA GGGAGAAAAC CTGTCAGAAC TTTCCATACG 2401 AGTATATCAG AACACACCCT TCCAAGGTAT GTATGCTCTG TTGTTCCTGT 2451 CCTGTCTTCA CTGAGCGCAG GGCTGGAGGC CTCTTAGACA TTCTCCTTGG 2501 TCCTCGTTCA GCTGCCCACT GTAGTATCCA CAGTGCCCGA GTTCTCGCTG 2551 GTTTTGGCAA TTAAACCTCC TTCCTACTGG TTTAGACTAC ACTTACAACA 2601 AGGAAAATGC CCCTCGTGTG ACCATAGATT GAGATTTATA CCACATACCA 2651 CACATAGCCA CAGAAACATC ATCTTGAAAT AAAGAAGAGT TTTGGACAAA 2701 AAAAAAAAAA AAAA BLAST Results Entry AF015416 from database EMBL: Homo sapiens chromosome 11 from llpl5.5 region, complete sequence. Score = 3356, P = 2.0e-144, identities = 672/673 Entry HS263253 from database EMBL: human STS SHGC-15914. Score = 1143, P = 9.0e-46, identities = 245/255 Medline entries No Medline entry Peptide information for frame 2 ORF from 326 bp to 1936 bp; peptide length: 537 Category: similarity to unknown protein 1 MEPRAVAEAV ETGEEDVIME ALRSYNQEHS QSFTFDDAQQ EDRKRLAELL 51 VSVLEQGLPP SHRVIWLQSV RILSRDRNCL DPFTSRQSLQ ALACYADISV 101 SEGSVPESAD MDVVLESLKC LCNLVLSSPV AQMLAAEARL VVKLTERVGL 151 YRERSFPHDV QFFDLRLLFL LTALRTDVRQ QLFQELKGVR LLTDTLELTL 201 GVTPEGNPPP TLLPSQETER AMEILKVLFN ITLDSIKGEV DEEDAALYRH 251 LGTLLRHCVM IATAGDRTEE FHGHAVNLLG NLPLKCLDVL LTLEPHGDST 301 EFMGVNMDVI RALLIFLEKR LHKTHRLKES VAPVLSVLTE CARMHRPARK 351 FLKAQGWPPP QVLPPLRDVR TRPEVGEMLR NKLVRLMTHL DTDVKRVAAE 401 FLFVLCΞESV PRFIKYTGYG NAAGLLAARG LMAGGRPEGQ YSEDEDTDTD 451 EYKEAKASIN PVTGRVEEKP PNPMEGMTEE QKEHEAMKLV TMFDKLSRNR 501 VIQPMGMSPR GHLTSLQDAM CETMEQQLSS DPDSDPD BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphutel_22o2, frame 2 TREMBL: SPBC3E7_3 gene: "SPBC3E7.03c"; product: "hypothetical protein"; S. pombe chromosome II cosmid c3E7. , N = 1, Score = 112, P = 0.0023 >TREMBL:SPBC3E7_3 gene: "SPBC3E7.03c"; product: "hypothetical protein"; S. pombe chromosome II cosmid c3E7. Length = 362 HSPs: Score = 112 (16.8 bits), Expect = 2.3e-03, P = 2.3e-03 Identities = 71/289 (24%), Positives = 124/289 (42%) Query: 215 SQETERAM-EILKVLFNITLDSIKGEVDEEDAALYRHLGTLLRHCVMIATAGDRTEEFHG 273 SQ+ E + EIL++LF 1+ S E DE+ L L+ + + Sbjct: 12 ΞQDNEMVLTEILRLLFPISKRSYLKEEDEQKILL LVIEIWASSLNNNPNSPLRW 65 Query: 274 HAVN-LLG-NLPLKCLDVLLTLEPHGDSTEFMGVNMDVIRALLIFLEKRLHKTH RL 327 HA N LL NL L LD + + T + +1 + +LEK L+ + Sbjct: 66 HATNALLSFNLQLLSLDQAIYVSEIACQT LQSILISREVEYLEKGLNLCFDIAAKY 121 Query: 328 KESVAPVLSVLTECARMHRPARKFLKAQGWPPPQVLPPLRDVRTRP-EVGEMLRNKLVRL 386 + ++ P+L++L + +L P D R + + G+ R L+RL Sbjct: 122 QNTLPPILAILLSLLSFFNIKQNL SMLLFPTNDDRKQSLQKGKSFRCLLLRL 173 Query: 387 MT-HLDTDVKRVAAEFLFVLCSESVPRFIKYTGYGNAAGLLAARGLMAGGRPEGQYΞ 442 +T + + A L LC + + G G A G+ M P + + Sbjct: 174 LTIPIVEPIGTYYASLLNELCDGDSQQIARIFGAGYAMGISQHSETMPFPSPLSKAASPV 233 Query: 443 -EDEDTDTDEYKEAKASINPVTGRV—EEKPPNPMEGMTEEQKEHEAMKLVTMFDKLSRN 499 + + +E +I+P+TG + +E +++E+KE EA +L +F +L +N Sbjct: 234 FQKNSRGQENTEENNLAIDPITGSMCTNRNKSQRLE-LSQEEKEREAERLFYLFQRLEKN 292 Query: 500 RVIQ 503 IQ Sbjct: 293 STIQ 296 Pedant information for DKFZphutel_22o2, frame 2 Report for DKFZphutel_22o2.2 [LENGTH] 537 [MW] 6037 2.53 [pi] 5.20 [BLOCKS] BL00 415L Synapsms proteins [PROSITE] MYRI STYL 4 [PROSITE] CK2 PHOSPHO_SITE 13 [PROSITE] PKC PHOSPHO_SITE 10 [PROSITE] ASN GLYCOSYLATION 1 [KW] All Alpha [KW] LOW ' COMPLEXITY 9.50 % SEQ MEPRAVAEAVETGEEDVIMEALRSYNQEHSQSFTFDDAQQEDRKRLAELLVSVLEQGLPP SEG PRD ccchhhhhhhhhccchhhhhhhhhhccccccceeeccchhhhhhhhhhhhhhhhhccccc SEQ SHRVIWLQSVRILSRDRNCLDPFTSRQSLQALACYADISVSEGSVPESADMDVVLESLKC SEG PRD cceeeeeccccccccccccccccchhhhhhhhhhhhceeeeccccccccchhhhhhhhhh SEQ LCNLVLSSPVAQMLAAEARLVVKLTERVGLYRERSFPHDVQFFDLRLLFLLTALRTDVRQ SEG xxxxxxxxxxxxxxx ... PRD hhhhccccchhhhhhhhhhhhhhhhhccccccccccccccchhhhhhhhhhhhhhhhhhh SEQ QLFQELKGVRLLTDTLELTLGVTPEGNPPPTLLPSQETERAMEILKVLFNITLDSIKGEV SEG PRD hhhhhhchhhhhhhhhhhhccccccccccccccchhhhhhhhhhhhhhhhhhccccchhh SEQ DEEDAALYRHLGTLLRHCVMIATAGDRTEEFHGHAVNLLGNLPLKCLDVLLTLEPHGDST SEG PRD hhhhhhhhhhhhhhhhhhhhccccccccccccceeeeecccccccceeeeeeeccccccc SEQ EFMGVNMDVIRALLIFLEKRLHKTHRLKESVAPVLSVLTECARMHRPARKFLKAQGWPPP SEG PRD eeeehhhhhhhhhhhhhhhhhhhhhhccccceeeehhhhhhhhhchhhhhhhhccccccc SEQ QVLPPLRDVRTRPEVGEMLRNKLVRLMTHLDTDVKRVAAEFLFVLCSESVPRFIKYTGYG SEG xxx PRD cccccccccccchhhhhhhhhhhhhhhhhccchhhhhhhhhhhhhhcccccceeeecccc SEQ NAAGLLAARGLMAGGRPEGQYSEDEDTDTDEYKEAKASINPVTGRVEEKPPNPMEGMTEE SEG xxxxxxxxxxxxxxx xxxxxxxxx PRD chhhhhhhhhccccccccccccccccccchhhhhhhhhccccccceeecccccccchhhh SEQ QKEHEAMKLVTMFDKLSRNRVIQPMGMSPRGHLTSLQDAMCETMEQQLSSDPDSDPD SEG xxxxxxxxx PRD hhhhhhhhhhhhhhhcccccccccccccccccchhhhhhhhhhhhhhhhcccccccc Prosite for DKFZphutel_22o2.2 PS00001 230->234 ASN GLYCOSYLATION PDOC00001 PS00005 61->64 PKC _PHOSPHO_SITE PDOC00005 PS00005 69->72 PKC PHOSPHO_SITE PDOC00005 PΞ00005 84->87 PKC ~ PHOSPHO_SITE PDOC00005 PS00005 117-M20 PKC PHOSPHO_SITE PDOC00005 PS00005 145->148 PKC _ PHOSPHO_SITE PDOC00005 PS00005 218->221 PKC " PHOSPHO_SITE PDOC00005 PS00005 235->238 PKC ~ PHOSPHO_SITE PDOC00005 PS00005 324->327 PKC PHOSPHO_SITE PDOC00005 PS00005 463->466 PKC " PHOSPHO_SITE PDOC00005 PS00005 508->511 PKC PHOSPHO_SITE PDOC00005 PS00006 12->16 CK2 " PHOSPHO_SITE PDOC00006 PS00006 34->38 CK2 PHOSPHO_SITE PDOC00006 PS00006 52->56 CK2 " PHOSPHO_SITE PDOC00006 PS00006 99->103 CK2 PHOSPHO_SITE PDOC00006 PS00006 104->108 CK2 " PHOSPHO_SITE PDOC00006 PS00006 263->267 CK2 " PHOSPHO_SITE PDOC00006 PS00006 371->375 CK2 " PHOSPHO SITE PDOC00006 PS00006 388->392 CK2_PHOSPHO_SITE PDOC00006 PS00006 442->446 CK2_PHOSPHO_SITE PDOC00006 PS00006 447->451 CK2_PHOSPHO_SITE PDOC00006 PS00006 491->495 CK2_PHOSPHO_SITE PDOC00006 PS00006 515->519 CK2_PHOSPHO_SITE PDOC00006 PS00006 530->534 CK2_PHOΞPHO_SITE PDOC00006 PS00008 57->63 MYRISTYL PDOC00008 PS00008 420->426 MYRISTYL PDOC00008 PS00008 424->430 MYRISTYL PDOC00008 PS00008 430->436 MYRISTYL PDOC00008 (No Pfam data available for DKFZphutel_22o2.2) DKFZphutel_23el3 group: metabolism DKFZphtes3_15j 18 encodes a novel 148 amino acid protein with similarity to 27K heat shock proteins . The novel protein contains a serine protease of the subtilase family w th an aspartic acid- conta nmg active site. Subtilases are an extensive family of serine proteases whose catalytic activity is provided by a charge relay system similar to that of the trypsin family of serine proteases but which evolved by independent convergent evolution. The sequence around the residues involved in the catalytic triad (aspartic acid, serine and histidine) are completely different from that of the analogous residues in the trypsin serine proteases. Thus the novel protein is a new member of this family. The new protein can find application in modulation of proteinase activity in cells and as a new enzyme for proteomics and biotechnologic production processes. heat shock protein HSP27 strong similarity to heat shock 27K proteins complete cDNA, complete eds, EST hits Sequenced by EMBL Locus: /map="578.9 cR from top of Chrl2 linkage group" Insert length: 1854 bp Poly A stretch at pos. 1831, polyadenylation signal at pos. 1810 1 GGTTTATTAA GCTCCTGGCT CCGCTCTAGA CCTCAGCGGT TCTGGCTGCC 51 AGCCTGGGCA GCCTGGGAAG CCTGGGAGGA CGGTGGCTTG CCGGTCTGTC 101 GTGAGGCAGT GCGGACGGGG ACCCTCTGGG ATTCTGCTGG ATCTGCCCCG 151 GGGGTTACCT TTGGGGGCTG GGACCCCAGT CGAGGGGACA CAACCGTCCC 201 TGGCAGTGGT TGGTTCTGCT TCTCCCTGCA GAAAAGCAGC ATTTTCGGAA 251 GCTGAAGAAT AAGCTAGCCC AGCCACACCA CCTTGTTGTG TGACCTTGGG 301 CAGGTGGTTC TGTCTCTCTG AGCCTCTGTT TCTCTCTGAG CTGAGCAGCC 351 ACCATGGCTG ACGGTCAGAT GCCCTTCTCC TGCCACTACC CAAGCCGCCT 401 GCGCCGAGAC CCCTTCCGGG ACTCTCCCCT CTCCTCTCGC CTGCTGGATG 451 ATGGCTTTGG CATGGACCCC TTCCCAGACG ACTTGACAGC CTCTTGGCCC 501 GACTGGGCTC TGCCTCGTCT CTCCTCCGCC TGGCCAGGCA CCCTAAGGTC 551 GGGCATGGTG CCCCGGGGCC CCACTGCCAC CGCCAGGTTT GGGGTGCCTG 601 CCGAGGGCAG GACCCCCCCA CCCTTCCCTG GGGAGCCCTG GAAAGTGTGT 651 GTGAATGTGC ACAGCTTCAA GCCAGAGGAG TTGATGGTGA AGACCAAAGA 701 TGGATACGTG GAGGTGTCTG GCAAACATGA AGAGAAACAG CAAGAAGGTG 751 GCATTGTTTC TAAGAACTTC ACAAAGAAAA TCCAGCTTCC TGCAGAGGTG 801 GATCCTGTGA CAGTATTTGC CTCACTTTCC CCAGAGGGTC TGCTGATCAT 851 CGAAGCTCCC CAGGTCCCTC CTTACTCAAC ATTTGGAGAG AGCAGTTTCA 901 ACAACGAGCT TCCCCAGGAC AGCCAGGAAG TCACCTGTAC CTGAGATGCC 951 AGTACTGGCC CATCCTTGTT TTGTCCCCAA CCCTAGGGCT TCTCTGATTC 1001 CAGGATACAT TACTTTAGCT GAACTCAGAT TTAGTGCAAG TAAAATGTTA 1051 GAGGGTGCGG GGGTGAGGAC TGACCACAGA TTCCCTGGAT AGTGTAGTGG 1101 TAGATTTCTC CACAGGATAG CGCAATTGGC AAATCATGCT TGGTTGTGTT 1151 AGGCCAAAAT ACTAGTTTTG CTTTCTTTAC CTTTTCTATC TTGATGAAAA 1201 TGTTGCACAT TCTATAGTTG CAAAACACAT AAAAGGGGAC TTAACATTTC 1251 ACGTTGTATC TTACTTGCAG TGAATGCAAG GGTTACTTTT CTCTGGGGAC 1301 CTCCCCCATC ACCCAGGTTC CTACTCTGGG CTCCCGATTC CCATGGCTCC 1351 CAAACCATGC CGCATGGTTT GGTTAATGAA ACCCAGTAGC TAACCCCACT 1401 GTGCTTCCAC ATGCCTGGCC TAAAATGGGT GATATACAGG TCTTATATCC 1451 CCATATGGAA TTTATCCATC AACCACATAA AAACAAACAG TGCCTTCTGC 1501 CCTCTGCCCA GATGTGTCCA GCACGTTCTC AAAGTTTCCA CATTAGCACT 1551 CCCTAAGGAC GCTGGGAGCC TGTCAGTTTA TGATCTGACC TAGGTCCCCC 1601 CTTTCTTCTG TCCCCTGTGT TTAAGTCGGG ATTTTTACAG AGGGAGCTGT 1651 CTCCAGACAG CTCCATCAGG AACCAAGCAA AGGCCAGATA GCCTGACAGA 1701 TAGGCTAGTG GTATTGTGTA TATGGGCGGG ACGTGTGTGT CATTATTATT 1751 TGAGTTATGC TGTTGTTTAG GGGTAAATAA CAGTAAATAA TTAATAATAA 1801 TAATAATAAT AATAAAGGAG CTGACGTTCT TAAAAAAGAA AAAAAAAAAA 1851 AAAA BLAST Results Entry HS286348 from database EMBL: human STS TIGR-A002J47. Score = 510, P = 1.2e-16, identities = 102/102 Medline entries 95394379: Cloning and sequencing of a cDNA encoding the canine HSP27 protein. 94110260: Physiological and pathological changes in levels of the two small stress proteins, HSP27 and alpha B crystallin, in rat hindlimb muscles Peptide information for frame 3 ORF from 354 bp to 941 bp; peptide length: 196 Category: strong similarity to known protein Prosite motifs: SUBTILASE ASP (28-39) 1 MADGQMPFSC HYPSRLRRDP FRDSPLSSRL LDDGFGMDPF PDDLTASWPD 51 WALPRLSSAW PGTLRΞGMVP RGPTATARFG VPAEGRTPPP FPGEPWKVCV 101 NVHSFKPEEL MVKTKDGYVE VSGKHEEKQQ EGGIVSKNFT KKIQLPAEVD 151 PVTVFASLSP EGLLIIEAPQ VPPYSTFGES SFNNELPQDS QEVTCT BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphutel_23el3, frame 3 PIR:JC4244 heat-shock 27K protein - dog, N = 1, Score = 304, P = 4.3e-27 PIR:JN0924 heat shock 27 protein - rat, N = 1, Score = 301, P = 8.9e-27 TREMBL :MM03561_1 product: "heat shock protein HSP27"; Mus musculus heat shock protein HSP27 internal deletion variant b mRNA, complete eds., N = 1, Score = 301, P = 8.9e-27 >PIR:JC4244 heat-shock 27K protein - dog Length = 209 HSPs: Score = 304 (45.6 bits), Expect = 4.3e-27, P = 4.3e-27 Identities = 80/182 (43%), Positives = 102/182 (56%) Query: 1 MADGQMPFSC-HYPSRLRRDPFRD-SPLSSRLLDDGFGMDPFPDDLTASWPDWALPRLSS 58 M + ++PFS PS DPFRD P SRL D FG+ P++ W W S Sbjct: 1 MTERRVPFSLLRSPSW DPFRDWYPAHSRLFDQAFGLPRLPEE WAQWFG HS 50 Query: 59 AWPGTLRSGMVP RGPTATARFGVPAEGR—TPPPFPG EPWKVCVNVHSF 105 WPG +R +P GP A A PA R + G + W+V ++V+ F Sbjct: 51 GWPGYVRP—IPPAVEGPAAAAAAAAPAYSRALSRQLSSGVSEIRQTADRWRVSLDVNHF 108 Query: 106 KPEELMVKTKDGYVEVSGKHEEKQQEGGIVSKNFTKKIQLPAEVDPVTVFASLSPEGLLI 165 PEEL VKTKDG VE++GKHEE+Q E G +S+ T K LP VDP V +SLSPEG L Sbjct: 109 APEELTVKTKDGVVEITGKHEERQDEHGYISRRLTPKYTLPPGVDPTLVSSSLSPEGTLT 168 Query: 166 IEAPQVPPYSTFGE 179 +EAP P + E Sbjct: 169 VEAPMPKPATQSAE 182 Pedant information for DKFZphutel_23el3, frame 3 Report for DKFZphutel_23el3.3 [LENGTH] 196 [MW] 21604.37 [pi] 5.00 [HOMOL] PIR:JC4244 heat-shock 27K protein - dog 3e-22 [BLOCKS] BL01031C [PIRKW] blocked ammo end le-13 [PIRKW] acetylated ammo end 4e-13 [PIRKW] phosphoprotem 7e-21 [PIRKW] glycoprotein 2e-ll [PIRKW] heat shock 7e-21 [PIRKW] molecular chaperone 4e-13 [PIRKW] alternative splicing le-19 [PIRKW] eye lens 6e-14 [PIRKW] stress-induced protein 7e-21 [SUPFAM] alpha-crystallin 7e-21 [PROSITE] SUBTILASE_ASP 1 [PROSITE] MYRISTYL 2 [PROSITE] CK2_PHOSPHO_SITE 2 [PROSITE] PKC_PHOSPHO_SITE 6 [PROSITE] ASN_GLYCOSYLATION 1 [PFAM] Heat shock hsp20 proteins [KW] All_Beta [KW] LOW COMPLEXITY 7.14 % SEQ MADGQMPFΞCHYPSRLRRDPFRDSPLSSRLLDDGFGMDPFPDDLTASWPDWALPRLSSAW SEG xxxxxxxxxxxxxx PRD ccccccccccccccccccccccccccchhhhhcccccccccccccccccccccccccccc SEQ PGTLRSGMVPRGPTATARFGVPAEGRTPPPFPGEPWKVCVNVHSFKPEELMVKTKDGYVE SEG PRD cccccccccccccchhhhhhhhccccccchhhhhhheeeeeecccccceeeeecccceee SEQ VSGKHEEKQQEGGIVSKNFTKKIQLPAEVDPVTVFAΞLSPEGLLIIEAPQVPPYSTFGES SEG PRD eccchhhhhcccceeeeccccccccccccccceeeecccccceeeeeccccccccccccc SEQ SFNNELPQDSQEVTCT SEG PRD cccccccccceeeccc Prosite for DKFZphutel_23el3.3 PS00001 138->142 ASN_GLYCOSYLATION PDOC00001 PS00005 27->30 PKC_PHOSPHO_SITE PDOC00005 PS00005 63->66 PKC_PHOSPHO_SITE PDOC00005 PS00005 76->79 PKC_PHOSPHO_SITE PDOC00005 PS00005 104->107 PKC_PHOSPHO_SITE PDOC00005 PS00005 122->125 PKC_PHOSPHO_SITE PDOC00005 PS00005 140->143 PKC_PHOSPHO_SITE PDOC00005 PS00006 47->51 CK2_PHOSPHO_SITE PDOC00006 PS00006 176->180 CK2_PHOSPHO_SITE PDOC00006 PS00008 62->68 MYRISTYL PDOC00008 PS00008 132->138 MYRISTYL PDOC00008 PS00136 28->39 SUBTILASE ASP PDOC00125 Pfam for DKFZphutel_23el3.3 HMM_NAME Heat shock hsp20 proteins HMM *AMMrpPWDWRE DpDHFeVrMDMPGFKPEEIKVkVEDNNVLvIeG A P++ R + ++V++++ FKPEE+ VK+ D+ +++++G Query 77 ARFGVPAEGR-TPPPFPGEPWKVCVNVHSFKPEELMVKTKDG-YVEVSG 123 HMM EHEREEEREDDkWWWHERIYRHFMRRFrLPENVDpDqlkAsMSdNGVLTI +HE E++ + + ++ F +++LP +VDP + AS+S++G+L I Query 124 KHE EKQQ EGGIVSKNFTKKIQLPAEVDPVTVFASLSPEGLLII 166 HMM TVPKpEP* ++P ++P Query 167 EAPQVPP 173 DKFZphutel_23gll group: uterus derived DKFZphutel_23gll encodes a novel 256 amino acid protein with similarity to S. pombe SPAC31G5.12c and S. cerevisiae Maflp. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application m studying the expression profile of uterus-specific genes . similarity to SPAC31G5.12c and Maflp complete cDNA, complete eds, EST hits Sequenced by EMBL Locus : unknown Insert length: 1674 bp Poly A stretch at pos. 1664, polyadenylation signal at pos. 1644 1 GGGGGAGGCG GAGGTCGCTC GCTCGCTCGC TCGGCTCGCT GACTCGCCGG 51 AGCGCTCTGT GGCGGTCGGC GGCAGGTCGG TCGCGAGAGC GGGCTCTGTG 101 GAAGGGGGCG AGGCTATGTC GCGGTGGCAG CCCGGATGGG CCGGCAGGGC 151 CGGGAGTAAC GGGACGTCGC CGCGGAGCTT CTTCCCCCGG ATACAGTGCG 201 GCCCGAGCGG AGGCCGCGGC GCCGCCCTCC GATCTTGAAG AGCCCGCGCT 251 GCGCGGAGCC CGCCCCCGCC TGCGCACCGG CACCGACGCG GAGCGACCAG 301 CCCAGCCAGA CCCGGCCCGG CGCGGCCTGA TCTAACCCAG CCAGGCAGGC 351 AATACTAGCC CCTCTGGAGC ACGGAGCTCC TTCCCCAAAG ACATGAAGCT 401 ATTGGAGAAC TCGAGCTTTG AAGCCATCAA CTCACAGCTG ACTGTGGAGA 451 CCGGAGATGC CCACATCATT GGCAGGATTG AGAGCTACTC ATGTAAGATG 501 GCAGGAGACG ACAAACACAT GTTCAAGCAG TTCTGCCAGG AGGGCCAGCC 551 CCACGTGCTG GAGGCACTTT CTCCACCCCA GACTTCAGGA CTGAGCCCCA 601 GCAGACTCAG CAAAAGCCAA GGCGGTGAGG AGGAGGGCCC CCTCAGTGAC 651 AAGTGCAGCC GCAAGACCCT CTTCTACCTG ATTGCCACGC TCAATGAGTC 701 CTTCAGGCCT GACTATGACT TCAGCACAGC CCGCAGCCAT GAGTTCAGCC 751 GGGAGCCCAG CCTTAGCTGG GTGGTGAATG CAGTCAACTG CAGTCTGTTC 801 TCAGCTGTGC GGGAGGACTT CAAGGATCTG AAACCACAGC TGTGGAACGC 851 GGTGGACGAG GAGATCTGCC TGGCTGAATG TGACATCTAC AGCTATAACC 901 CAGACTTGGA CTCAGATCCC TTCGGGGAGG ATGGTAGCCT CTGGTCCTTC 951 AACTACTTCT TCTACAACAA GCGGCTCAAG CGAATCGTCT TCTTTAGCTG 1001 CCGTTCCATC AGTGGCTCCA CCTACACACC CTCAGAGGCA GGCAACGAGC 1051 TGGACATGGA GCTGGGGGAG GAGGAGGTGG AGGAAGAAAG CAGAAGCAGG 1101 GGCAGTGGGG CCGAGGAGAC CAGCACCATG GAGGAGGACA GGGTCCCAGT 1151 GATCTGTATT TGATGAGGAG GAGCCGAGGC CCCAGCTTCA TCCAGCTTCA 1201 ACCAATGCCT GGACCTGTCC ACCTGAGAGG CCCCTGGGGC CTCCCCAGCT 1251 GCTGGCCAGA CCCTGGCGCT GCCACAGTCC TGGCACTGCC CAAGGCCATA 1301 CCTGCCTAGC CCTTTGGCTC CATCCTGTGG ATGCCCACTC ACCCCTCAGA 1351 CTCCTGCTGC CCATGCTGTG GCCGGACTTG TCAGCAGGGG GCCTGGTGGG 1401 AGGAGCGACT GCCCTGCCCA AATGAACTGC CACAGCAGGG ACAGCTGGAC 1451 CGCAGAGTTT ATTTTTGTAT TTCTACTGGG CCTGCACACT CCAGCCCAAA 1501 GGGTCTGTGG CCGGAGGCCC CACGAGCAGG CCCCAGCAGT CACCGGCTCT 1551 GGTCTTGGGC CGGCCCCGGT GCCCACCTGT ACCCCCACCT CGCCCATTTG 1601 GCCGCGTGCA CTGAGTGTCA CTTTGCTGCA GCTCGTTTCT TTCCAATAAA 1651 AGTTTCTGTG ACTTAAAAAA AAAA BLAST Results No BLAST result Medlme entries No Medline entry Peptide information for frame 3 ORF from 393 bp to 1160 bp; peptide length: 256 Category: similarity to known protein 1 MKLLENSSFE AINSQLTVET GDAHIIGRIE SYSCKMAGDD KHMFKQFCQE 51 GQPHVLEALS PPQTSGLSPS RLSKSQGGEE EGPLSDKCSR KTLFYLIATL 101 NESFRPDYDF STARSHEFSR EPSLSWVVNA VNCSLFSAVR EDFKDLKPQL 151 WNAVDEEICL AECDIYSYNP DLDSDPFGED GΞLWSFNYFF YNKRLKRIVF 201 FSCRSISGST YTPSEAGNEL DMELGEEEVE EESRΞRGSGA EETSTMEEDR 251 VPVICI BLASTP hits Entry SPAC31G5_12 from database TREMBL: gene: "SPAC31G5.12c"; product: "hypothetical protein"; S. pombe chromosome I cosmid c31G5. Score = 272, P = 9.3e-24, identities = 51/127, positives = 80/127 Entry SPD656_1 from database TREMBL: product: "ORF N150"; Yeast DNA for bfr2+ proteιn/padl+ protem/sksl+ protein, ORF N313, ORF N150, complete eds, and for ORF N118, partial eds . Score = 263, P = 8.4e-23, identities = 50/127, positives = 79/127 Entry S50986 from database PIR: MAF1 protein - yeast (Saccharomyces cerevisiae) >SWIΞSPROT :MAF1_YEAST MAF1 PROTEIN. >TREMBL : SC19492_1 gene: "MAF1"; product: "Maflp"; Saccharomyces cerevisiae Maflp (MAF1) gene, complete eds. >TREMBL:SC8119_11 gene: "MAFlp"; product: "Maflp"; S. cerevisiae chromosome IV cosmid 8119. Score = 180, P = 2.3e-17, identities = 43/133, positives = 75/133 Entry AF098499_2 from database TREMBL: gene: "C43H8.2"; Caenorhabditis elegans cosmid C43H8. Score = 263, P = 9.2e-23, identities = 78/252, positives = 118/252 Alert BLASTP hits for DKFZphutel_23gll, frame 3 No Alert BLASTP hits found Pedant information for DKFZphutel_23gll, frame 3 Report for DKFZphutel_23gll .3 [LENGTH] 256 [MW] 28869.95 [pi] 4.51 [HOMOL] TREMBL :SPAC31G5_12 gene: "SPAC31G5.12c"; product: "hypothetical protein"; S. pombe chromosome I cosmid C31G5. 4e-23 [FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YDR005c] 6e-13 [PROSITE] MYRISTYL 3 [PROSITE] CK2_PHOSPHO_SITE 5 [PROSITE] PKC_PHOSPHO_SITE 6 [PROSITE] ASN_GLYCOSYLATION 3 [KW] All_Alpha [KW] LOW_COMPLEXITY 7.81 % SEQ MKLLENSSFEAINSQLTVETGDAHIIGRIESYSCKMAGDDKHMFKQFCQEGQPHVLEALS SEG PRD cccccchhhhhhhhhhhhccccceeeeecccchhhhhccchhhhhhhhhcccceeeeccc SEQ PPQTSGLSPSRLSKSQGGEEEGPLSDKCSRKTLFYLIATLNEΞFRPDYDFSTARSHEFSR SEG PRD cccccccccccccccccccccccccccchhhhhhhhhhhhcccccccccccccccccccc SEQ EPSLSWVVNAVNCSLFSAVREDFKDLKPQLWNAVDEEICLAECDIYSYNPDLDSDPFGED SEG PRD ccccccchhhhhhhhhhhhhchhhhhhhhhhhhhhhhccccccceeeccccccccccccc SEQ GSLWΞFNYFFYNKRLKRIVFFSCRSISGSTYTPSEAGNELDMELGEEEVEEESRSRGSGA SEG xxxxxxxxxxxxxxxxxx PRD ccceeeceeechhhhhhhhhhhccccccccccccccccchhhhhhhhhhhhhhccccccc SEQ EETSTMEEDRVPVICI SEG XX PRD cccccccccceeeccc Prosite for DKFZphutel_23gll .3 PS00001 6->10 ASN_GLYCOSYLATION PDOC00001 PS00001 101->105 ASN_GLYCOSYLATION PDOC00001 PS00001 132->136 ASN_GLYCOSYLATION PDOC00001 PS00005 33->36 PKC_PHOSPHO_SITE PDOC00005 PS00005 85->88 PKC_PHOSPHO_SITE PDOC00005 PS00005 89->92 PKC_PHOSPHO_SITE PDOC00005 PS00005 103->106 PKC_PHOSPHO_SITE PDOC00005 PS00005 112->115 PKC_PHOSPHO_SITE PDOC00005 PS00005 202->205 PKC_PHOSPHO_SITE PDOC00005 PS00006 7->ll CK2_PHOSPHO_SITE PDOC00006 PS00006 99->103 CK2_PHOSPHO_SITE PDOC00006 PS00006 212->216 CK2_PHOSPHO_SITE PDOC00006 PS00006 238->242 CK2_PHOSPHO_SITE PDOC00006 PS00006 244->248 CK2_PHOSPHO_SITE PDOC00006 PS00008 66->7 2 MYRISTYL PDOC00008 PS00008 181-M87 MYRISTYL PDOC00008 PS00008 239->245 MYRISTYL PDOC00008 (No Pfam data available for DKFZphutel_23gll .3) DKFZphutel_24cl9 group: transmembrane protein DKFZphutel_24cl9 encodes a novel 195 amino ac d protein without similarity to known proteins. The novel protein contains 1 transmembrane region. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of uterus-specific genes and as a new marker for uterine cells. unknown membrane regions: 1 Summary DKFZphutel_24cl9 encodes a novel 195 amino acid protein, with no similarity to known proteins. unknown complete cDNA, complete eds, EST hits TRANSMEMBRANE 1 Sequenced by Qiagen Locus: unknown Insert length: 769 bp Poly A stretch at pos. 746, polyadenylation signal at pos. 735 1 ACGAGTCAGC CAAAGATGGC TGCGCCCAGG TAATTTGAGC AAAGGCCACA 51 GTGAACTCCG GCGTGGCTGA GGAAGACCGG AGGAGGCACC CACAGGCTGC 101 TGGGAGGAGA GCATAAGGCT CAAAATGGAA AATCATAAAT CCAATAATAA 151 GGAAAACATA ACAATTGTTG ATATATCCAG AAAAATTAAC CAGCTTCCAG 201 AAGCAGAAAG GAATCTACTT GAAAATGGAT CGGTTTATGT TGGATTAAAT 251 GCTGCTCTTT GTGGCCTCAT AGCAAACAGT CTTTTTCGAC GCATCTTGAA 301 TGTGACAAAG GCTCGCATAG CTGCTGGCTT ACCAATGGCA GGGATACCTT 351 TTCTTACAAC AGACTTAACT TACAGATGTT TTGTAAGTTT TCCTTTGAAT 401 ACAGGTGATT TGGATTGTGA AACCTGTACC ATAACACGGA GTGGACTGAC 451 TGGTCTTGTT ATTGGTGGTC TATACCCTGT TTTCTTGGCT ATACCTGTAA 501 ATGGTGGTCT AGCAGCCAGG TATCAATCAG CTCTGTTACC ACACAAAGGG 551 AACATCTTAA GTTACTGGAT TAGAACTTCT AAGCCTGTCT TTAGAAAGAT 601 GTTATTTCCT ATTTTGCTCC AGACTATGTT TTCAGCATAC CTTGGGTCTG 651 AACAATATAA ACTACTTATA AAGGCCCTTC AGTTATCTGA ACCTGGCAAA 701 GAAATTCACT GATTTTAAAC AAATATGTAA ACAAAAATAA AATGGTAAAA 751 ACAAAAAAAA AAAAAAAAA BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 2 ORF from 125 bp to 709 bp; peptide length: 195 Category: putative protein 1 MENHKSNNKE NITIVDISRK INQLPEAERN LLENGSVYVG LNAALCGLIA 51 NSLFRRILNV TKARIAAGLP MAGIPFLTTD LTYRCFVSFP LNTGDLDCET 101 CTITRSGLTG LVIGGLYPVF LAIPVNGGLA ARYQSALLPH KGNILSYWIR 151 TSKPVFRKML FPILLQTMFS AYLGSEQYKL LIKALQLSEP GKEIH BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphutel_24cl9, frame 2 No Alert BLASTP hits found Pedant information for DKFZphutel_24cl9, frame 2 Report for DKFZphutel_24cl9.2 [LENGTH] 195 [MW] 21527.45 [pi] 9.36 [PROSITE] MYRISTYL 6 [PROSITE] CK2_PHOSPHO_SITE [PROSITE] PKC_PHOSPHO_SITE [PROSITE] ASN_GLYCOSYLATION [KW] TRANSMEMBRANE 1 SEQ MENHKSNNKENITIVDISRKINQLPEAERNLLENGSVYVGLNAALCGLIANSLFRRILNV PRD cccccccccceeeeeehhhhhhccchhhhhhhccccceeeecchhhhhhhhhhhhhhhhh MEM SEQ TKARIAAGLPMAGIPFLTTDLTYRCFVSFPLNTGDLDCETCTITRSGLTGLVIGGLYPVF PRD hhhhhhhccccccceeeeecccccccccccccccccccccccccccccceeeecccceee MEM MMMMMMMMMMMMMM SEQ LAIPVNGGLAARYQSALLPHKGNILSYWIRTSKPVFRKMLFPILLQTMFSAYLGSEQYKL PRD eeeccccccchhhhhhccccccceeeeeeecccchhhhhchhhhhhhhhhhhhcchhhhh MEM MMM SEQ LIKALQLSEPGKEIH PRD hhhhhhhcccccccc MEM Prosite for DKFZphutel J _24cl9.2 PS00001 11->15 ASN_GLYCOSYLATION PDOC00001 PS00001 34->38 ASN_GLYCOSYLATION PDOC00001 PS00001 59->63 ASN_GLYCOSYLATION PDOC00001 PS00005 18->21 PKC_PHOSPHO_SITE PDOC00005 PS00005 82->85 PKC_PHOSPHO_SITE PDOC00005 PS00005 151->154 PKC_PHOSPHO_SITE PDOC00005 PS00006 13->17 CK2_PHOSPHO_SITE PDOC00006 PS00008 40->46 MYRISTYL PDOC00008 PS00008 47->53 MYRISTYL PDOC00008 PS00008 68->74 MYRISTYL PDOC00008 PΞ00008 110->116 MYRISTYL PDOC00008 PS00008 127->133 MYRISTYL PDOC00008 PS00008 142->148 MYRISTYL PDOC00008 (No Pfam data available for DKFZphutel_24cl9.2) DKFZphutel 24ell group: intracellular transport and trafficking DKFZphutel_24ell encodes a novel 226 ammo acid protein, with similarity to human/mouse golgi 4-transmembrane spanning transporter MTP. MTP may function in the transport of nucleosides and/or nucleoside derivatives between the cytosol and the lumen of an intracellular membrane- bound compartment. Thus, the novel protein also seems to be involved in nucleotide sugar transport . The new protein can find application in modulating the transport of nucleosides and/or nucleoside derivatives between the cytosol and the lumen of an intracellular membrane-bound compartments . Similarity to 4-TRANSMEMBRANE SPANNING TRANSPORTER MTP complete cDNA, complete eds, EST hits potential start at 184, TRANSMEMBRANE 4 function in the transport of nucleosides and/or nucleoside derivatives between the cytosol and the lumen of an intracellular membrane-bound compartment 9 Sequenced by Qiagen Locus: /map="8" Insert length: 2005 bp Poly A stretch at pos. 1988, polyadenylation signal at pos. 1963 1 ACGCGTCCGG CAGAAGCTCG GAGCTCTCGG GGTATCGAGG AGGCAGGCCC 51 GCGGGCGCAC GGGCGAGCGG GCCGGGAGCC GGAGCGGCGG AGGAGCCGGC 101 AGCAGCGGCG CGGCGGGCTC CAGGCGAGGC GGTCGACGCT CCTGAAAACT 151 TGCGCGCGCG CTCGCGCCAC TGCGCCCGGA GCGATGAAGA TGGTCGCGCC 201 CTGGACGCGG TTCTACTCCA ACAGCTGCTG CTTGTGCTGC CATGTCCGCA 251 CCGGCACCAT CCTGCTCGGC GTCTGGTATC TGATCATCAA TGCTGTGGTA 301 CTGTTGATTT TATTGAGTGC CCTGGCTGAT CCGGATCAGT ATAACTTTTC 351 AAGTTCTGAA CTGGGAGGTG ACTTTGAGTT CATGGATGAT GCCAACATGT 401 GCATTGCCAT TGCGATTTCT CTTCTCATGA TCCTGATATG TGCTATGGCT 451 ACTTACGGAG CGTACAAGCA ACGCGCAGCC TGGATCATCC CATTCTTCTG 501 TTACCAGATC TTTGACTTTG CCCTGAACAT GTTGGTTGCA ATCACTGTGC 551 TTATTTATCC AAACTCCATT CAGGAATACA TACGGCAACT GCCTCCTAAT 601 TTTCCCTACA GAGATGATGT CATGTCAGTG AATCCTACCT GTTTGGTCCT 651 TATTATTCTT CTGTTTATTA GCATTATCTT GACTTTTAAG GGTTACTTGA 701 TTAGCTGTGT TTGGAACTGC TACCGATACA TCAATGGTAG GAACTCCTCT 751 GATGTCCTGG TTTATGTTAC CAGCAATGAC ACTACGGTGC TGCTACCCCC 801 GTATGATGAT GCCACTGTGA ATGGTGCTGC CAAGGAGCCA CCGCCACCTT 851 ACGTGTCTGC CTAAGCCTTC AAGTGGGCGG AGCTGAGGGC AGCAGCTTGA 901 CTTTGCAGAC ATCTGAGCAA TAGTTCTGTT ATTTCACTTT TGCCATGAGC 951 CTCTCTGAGC TTGTTTGTTG CTGAAATGCT ACTTTTTAAA ATTTAGATGT 1001 TAGATTGAAA ACTGTAGTTT TCAACATATG CTTTGCTAGA ACACTGTGAT 1051 AGATTAACTG TAGAATTCTT CCTGTACGAT TGGGGATATA ACGGGCTTCA 1101 CTAACCTTCC CTAGGCATTG AAACTTCCCC CAAATCTGAT GGACCTAGAA 1151 GTCTGCTTTT GTACCTGCTG GGCCCCAAAG TTGGGCATTT TTCTCTCTGT 1201 TCCCTCTCTT TTGAAAATGT AAAATAAAAC CAAAAATAGA CAACTTTTTC 1251 TTCAGCCATT CCAGCATAGA GAACAAAACC TTATGGAAAC AGGAATGTCA 1301 ATTGTGTAAT CATTGTTCTA ATTAGGTAAA TAGAAGTCCT TATGTATGTG 1351 TTACAAGAAT TTCCCCCACA ACATCCTTTA TGACTGAAGT TCAATGACAG 1401 TTTGTGTTTG GTGGTAAAGG ATTTTCTCCA TGGCCTGAAT TAAGACCATT 1451 AGAAAGCACC AGGCCGTGGG AGCAGTGACC ATCTACTGAC TGTTCTTGTG 1501 GATCTTGTGT CCAGGGACAT GGGGTGACAT GCCTCGTATG TGTTAGAGGG 1551 TGGAATGGAT GTGTTTGGCG CTGCATGGGA TCTGGTGCCC CTCTTCTCCT 1601 GGATTCACAT CCCCACCCAG GGCCCGCTTT TACTAAGTGT TCTGCCCTAG 1651 ATTGGTTCAA GGAGGTCATC CAACTGACTT TATCAAGTGG AATTGGGATA 1701 TATTTGATAT ACTTCTGCCT AACAACATGG AAAAGGGTTT TCTTTTCCCT 1751 GCAAGCTACA TCCTACTGCT TTGAACTTCC AAGTATGTCT AGTCACCTTT 1801 TAAAATGTAA ACATTTTCAG AAAAATGAGG ATTGCCTTCC TTGTATGCGC 1851 TTTTTACCTT GACTACCTGA ATTGCAAGGG ATTTTTATAT ATTCATATGT 1901 TACAAAGTCA GCAACTCTCC TGTTGGTTCA TTATTGAATG TGCTGTAAAT 1951 TAAGTCGTTT GCAATTAAAA CAAGGTTTGC CCACATCCAA AAAAAAAAAA 2001 AAAAA BLAST Results Entry HS012351 from database EMBL: human STS SHGC-31823. Score = 1629, P = 3. 1e-67 , identities = 343/354 Medline entries 96199248- Identification of a novel membrane transporter associated with intracellular membranes by phenotypic complementation in the yeast Saccharomyces cerevisiae. Peptide information for frame 1 ORF from 184 bp to 861 bp; peptide length: 226 Category: strong similarity to known protein 1 MKMVAPWTRF YSNSCCLCCH VRTGTILLGV WYLIINAWL LILLSALADP 51 DQYNFSSSEL GGDFEFMDDA NMCIAIAISL LMILICAMAT YGAYKQRAAW 101 IIPFFCYQIF DFALNMLVAI TVLIYPNSIQ EYIRQLPPNF PYRDDVMSVN 151 PTCLVLIILL FISIILTFKG YLIΞCVWNCY RYINGRNSSD VLVYVTΞNDT 201 TVLLPPYDDA TVNGAAKEPP PPYVSA BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphutel_24ell, frame 1 SWISSPROT:MTRP_HUMAN GOLGI 4-TRANSMEMBRANE SPANNING TRANSPORTER MTP (KIAA0108)., N = 1, Score = 551, P = 2.9e-53 SWISSPROT:MTRP_MOUΞE GOLGI 4-TRANSMEMBRANE SPANNING TRANSPORTER MTP., N = 1, Score = 539, P = 5.3e-52 TREMBL :HS304981_1 product: "E3 protein"; Human retmoic acid-mducible E3 protein mRNA, complete eds., N = 1, Score = 127, P = 3.4e-06 >SWISSPROT:MTRP_HUMAN GOLGI 4-TRANSMEMBRANE SPANNING TRANSPORTER MTP (KIAA0108) . Length = 233 HSPs: Score = 551 (82.7 bits), Expect = 2.9e-53, P = 2.9e-53 Identities = 102/221 (46%), Positives = 148/221 (66%) Query: 9 RFYSNSCCLCCHVRTGTILLGVWYLIINAVVLLILLSALADPDQY NFSSSELGGDF- 64 RFYS CC CCHVRTGTI+LG WY+++N ++ ++L + P+ N +G + Sbjct: 13 RFYSTRCCGCCHVRTGTIILGTWYMVVNLLMAILLTVEVTHPNSMPAVNIQYEVIGNYYS 72 Query: 65 -EFMDDANMCIAIAISLLMILICAMATYGAYKQRAAWIIPFFCYQIFDFALNMLVAITVL 123 E M D N C+ A+S+LM +1 +M YGA + W+IPFFCY++FDF L+ LVAI+ L Sbjct: 73 SERMAD-NACVLFAVSVLMFIISSMLVYGAISYQVGWLIPFFCYRLFDFVLSCLVAISSL 131 Query: 124 IYPNSIQEYIRQLPPNFPYRDDVMSVNPTCLVLIILLFISIILTFKGYLISCVWNCYRYI 183 Y I+EY+ QLP +FPY+DD+++++ +CL+ I+L+F ++ + FK YLI+CVWNCY+YI Sbjct: 132 TYLPRIKEYLDQLP-DFPYKDDLLALDSSCLLFIVLVFFALFIIFKAYLINCVWNCYKYI 190 Query: 184 NGRNSSDVLVYVTSN-DTTVLLPPYDDATVNGAAKEPPPPYVSA 226 N RN ++ VY +LP Y+ A V KEPPPPY+ A Sbjct: 191 NNRNVPEIAVYPAFEAPPQYVLPTYEMA-VKMPEKEPPPPYLPA 233 Pedant information for DKFZphutel_24ell, frame 1 Report for DKFZphutel_24ell .1 [LENGTH] 226 [MW] 25419.11 [pi] 4.65 [HOMOL] SWISSPROT:MTRP HUMAN GOLGI 4-TRANSMEMBRANE SPANNING TRANSPORTER MTP (KIAA0108) 5e-40 [PROSITE] CK2_PHOSPHO_SITE [PROSITE] TYR_PHOSPHO_SITE [PROSITE] PKC_PHOSPHO_SITE [PROSITE] ASN_GLYCOSYLATION [KW] SIGNAL_PEPTIDE 49 [KW] TRANSMEMBRANE 2 [KW] LOW COMPLEXITY 20.80 % SEQ MKMVAPWTRFYSNSCCLCCHVRTGTILLGVWYLIINAVVLLILLSALADPDQYNFSSSEL SEG xxxxxxxxxxxxxxxx PRD ccceeeeeeecccceeeeeeeeccceeecceeehhhhhhhhhhhhhhcccccceeecccc MEM SEQ GGDFEFMDDANMCIAIAISLLMILICAMATYGAYKQRAAWIIPFFCYQIFDFALNMLVAI SEG xxxxxxxxxxxxxxxxxx PRD ccccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhchhhhhhhhhhhhhhhhhhh MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMM SEQ TVLIYPNSIQEYIRQLPPNFPYRDDVMSVNPTCLVLIILLFISIILTFKGYLISCVWNCY SEG xxxxxxxxxxxxx PRD hhhcccchhhhhhhhcccccccccceeeeccccceeehhhhhhhhhhhhhheeeeeeeee MEM MMMMMM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM.... SEQ RYINGRNSSDVLVYVTSNDTTVLLPPYDDATVNGAAKEPPPPYVSA SEG PRD eecccccccceeeeeecccccccccccccccccccccccccccccc MEM Prosite for DKFZphutel_24ell .1 PS00001 54->58 ASN GLYCOSYLATION PDOC00001 PS00001 187->191 ASN GLYCOSYLATION PDOC00001 PS00001 198->202 ASN GLYCOSYLATION PDOC00001 PS00005 167->170 PKC PHOSPHO SITE PDOC00005 PS00006 56->60 CK2 PHOSPHO SITE PDOC00006 PS00006 128->132 CK2 PHOSPHO SITE PDOC00006 PS00006 196->200 CK2 PHOSPHO SITE PDOC00006 PS00007 186->195 TYR PHOSPHO SITE PDOC00007 (No Pfam data available for DKFZphutel_24ell .1) DKFZphutel_24j6 group: cell structure and motility DKFZphutesl_24j 6 encodes a novel 571 amino acid protein with strong similarity to rat cell adhesion regulator (CARD . The novel protein is very similar to Carl and thus seems to be involved in regulation cell- cell adhesion. It contains a RGD cell attachment site. The new protein can find application in modulation of cell-cell-adhesion. strong similarity to rat CAR1 A. thaliana T19C21.5 complete cDNA, complete eds, EST hits potential frame shift at Bp 1241 according to CAR1 but frame shift might be m CAR1 sequence 1 ESTs T73366 AA362984 confirm this sequence Sequenced by Qiagen Locus: /map="939.9 cR from top of Chr2 linkage group" Insert length: 3333 bp Poly A stretch at pos. 3316, no polyadenylation signal found 1 ACGCGTCCGA GCTGGCTCAG GGCGTCCGCT AGGCTCGGAC GACCTGCTGA 51 GCCTCCCAAA CCGCTTCCAT AAGGCTTTGC CTTTCCAACT TCAGCTACAG 101 TGTTAGCTAA GTTTGGAAAG AAGGAAAAAA GAAAATCCCT GGGCCCCTTT 151 TCTTTTGTTC TTTGCCAAAG TCGTCGTTGT AGTCTTTTTG CCCAAGGCTG 201 TTGTGTTTTT AGAGGTGCTA TCTCCAGTTC CTTGCACTCC TGTTAACAAG 251 CACCTCAGCG AGAGCAGCAG CAGCGATAGC AGCCGCAGAA GAGCCAGCGG 301 GGTCGCCTAG TGTCATGACC AGGGCGGGAG ATCACAACCG CCAGAGAGGA 351 TGCTGTGGAT CCTTGGCCGA CTACCTGACC TCTGCAAAAT TCCTTCTCTA 401 CCTTGGTCAT TCTCTCTCTA CTTGGGGAGA TCGGATGTGG CACTTTGCGG 451 TGTCTGTGTT TCTGGTAGAG CTCTATGGAA ACAGCCTCCT TTTGACAGCA 501 GTCTACGGGC TGGTGGTGGC AGGGTCTGTT CTGGTCCTGG GAGCCATCAT 551 CGGTGACTGG GTGGACAAGA ATGCTAGACT TAAAGTGGCC CAGACCTCGC 601 TGGTGGTACA GAATGTTTCA GTCATCCTGT GTGGAATCAT CCTGATGATG 651 GTTTTCTTAC ATAAACATGA GCTTCTGACC ATGTACCATG GATGGGTTCT 701 CACTTCCTGC TATATCCTGA TCATCACTAT TGCAAATATT GCAAATTTGG 751 CCAGTACTGC TACTGCAATC ACAATCCAAA GGGATTGGAT TGTTGTTGTT 801 GCAGGAGAAG ACAGAAGCAA ACTAGCAAAT ATGAATGCCA CAATACGAAG 851 GATTGACCAG TTAACCAACA TCTTAGCCCC CATGGCTGTT GGCCAGATTA 901 TGACATTTGG CTCCCCAGTC ATCGGCTGTG GCTTTATTTC GGGATGGAAC 951 TTGGTATCCA TGTGCGTGGA GTACGTCCTG CTCTGGAAGG TTTACCAGAA 1001 AACCCCAGCT CTAGCTGTGA AAGCTGGTCT TAAAGAAGAG GAAACTGAAT 1051 TGAAACAGCT GAATTTACAC AAAGATACTG AGCCAAAACC CCTGGAGGGA 1101 ACTCATCTAA TGGGTGTGAA AGACTCTAAC ATCCATGAGC TTGAACATGA 1151 GCAAGAGCCT ACTTGTGCCT CCCAGATGGC TGAGCCCTTC CGTACCTTCC 1201 GAGATGGATG GGTCTCCTAC TACAACCAGC CTGTGTTTCT GGCTGGCATG 1251 GGTCTTGCTT TCCTTTATAT GACTGTCCTG GGCTTTGACT GCATCACCAC 1301 AGGGTACGCC TACACTCAGG GACTGAGTGG TTCCATCCTC AGTATTTTGA 1351 TGGGAGCATC AGCTATAACT GGAATAATGG GAACTGTAGC TTTTACTTGG 1401 CTACGTCGAA AATGTGGTTT GGTTCGGACA GGTCTGATCT CAGGATTGGC 1451 ACAGCTTTCC TGTTTGATCT TGTGTGTGAT CTCTGTATTC ATGCCTGGAA 1501 GCCCCCTGGA CTTGTCCGTT TCTCCTTTTG AAGATATCCG ATCAAGGTTC 1551 ATTCAAGGAG AGTCAATTAC ACCTACCAAG ATACCTGAAA TTACAACTGA 1601 AATATACATG TCTAATGGGT CTAATTCTGC TAATATTGTC CCGGAGACAA 1651 GTCCTGAATC TGTGCCCATA ATCTCTGTCA GTCTGCTGTT TGCAGGCGTC 1701 ATTGCTGCTA GAATCGGTCT TTGGTCCTTT GATTTAACTG TGACACAGTT 1751 GCTGCAAGAA AATGTAATTG AATCTGAAAG AGGCATTATA AATGGTGTAC 1801 AGAACTCCAT GAACTATCTT CTTGATCTTC TGCATTTCAT CATGGTCATC 1851 CTGGCTCCAA ATCCTGAAGC TTTTGGCTTG CTCGTATTGA TTTCAGTCTC 1901 CTTTGTGGCA ATGGGCCACA TTATGTATTT CCGATTTGCC CAAAATACTC 1951 TGGGAAACAA GCTCTTTGCT TGCGGTCCTG ATGCAAAAGA AGTTAGGAAG 2001 GAAAATCAAG CAAATACATC TGTTGTTTGA GACAGTTTAA CTGTTGCTAT 2051 CCTGTTACTA GATTATATAG AGCACATGTG CTTATTTTGT ACTGCAGAAT 2101 TCCAATAAAT GGCTGGGTGT TTTGCTCTGT TTTTACCACA GCTGTGCCTT 2151 GAGAACTAAA AGCTGTTTAG GAAACCTAAG TCAGCAGAAA TTAACTGATT 2201 AATTTCCCTT ATGTTGAGGC ATGGAAAAAA AATTGGAAAA GAAAAACTCA 2251 GTTTAAATAC GGAGACTATA ATGATAACAC TGAATTCCCC TATTTCTCAT 2301 GAGTAGATAC AATCTTACGT AAAAGAGTGG TTAGTCACGT GAATTCAGTT 2351 ATCATTTGAC AGATTCTTAT CTGTACTAGA ATTCAGATAT GTCAGTTTTC 2401 TGCAAAACTC ACTCTTGTTC AAGACTAGCT AATTTATTTT TTTGCATCTT 2451 AGTTATTTTT AAAAACAAAT TCTTCAAGTA TGAAGACTAA ATTTTGATAA 2501 CTAATATTAT CCTTATTGAT CCTATTGATC TTAAGGTATT TACATGTATG 2551 TGGAAAAACA AAACACTTAA CTAGAATTCT CTAATAAGGT TTATGGTTTA 2601 GCTTAAAGAG CACCTTTGTA TTTTTATTAT CAGATGGGGC AACATATTGT 2651 ATGAAGCATA TGTAGCACTT CACAGCATGG TTATCATGTA AGCTGCAGGT 2701 AGAAGCAAAG CTGTAAAGTA GATTTATCAC ACAATGACTG CATACAGACT 2751 TCAAATATGT CAATAGTTTG GTCATAGAAC CTAGAAGCCA AAAGCCACAC 2801 AGAAGGGCAA GAATCCCAAT TTAACTCATG TTATCATCAT TAGTGATCTG 2851 TGTTGTAGAA CATGAGGGTG TAAGCCTTCA GCCTGGCAAG TTACATGTAG 2901 AAAGCCCACA CTTGTGAAGG TTTTGTTTTA CAAATCACTT GATTTAACAC 2951 ACTCAGGTAG AATATTTTTA TTTTTACTGT TTTATACCCA GAAGTTATTT 3001 CTACATTGTT CTACAGCAAG AATATTCATA AAAGTATCCC TTTCAAATGC 3051 CTTTGAGAAG AATAGAAGAA AAAAAGTTTG TATATATTTT AAAAAATTGT 3101 TTTAAAAGTC AGTTTGCAAC ATGTCTGTAC CAAGATGGTA CTTTGCCTTA 3151 ACCGTTTATA TGCACTTTCA TGGAGACTGC AATACGTTGC TATGAGCACT 3201 TTCTTTATCC TTGGAGTTTA ATCCTTTGCT TCATCTTTCT ACAGTATGAC 3251 ATAATGATTT GCTATGTTGT AAAATCTTTG TAAAAAATTT CTATATAAAA 3301 ATATTTTGAA AATCTTAAAA AAAAAAAAAA AAA BLAST Results Entry HS389210 from database EMBL: human STS SHGC-10164. Score = 1592, P = 1.5e-64, identities = 346/364 Entry HS933343 from database EMBL: human STS WI-16551. Score = 1193, P = 5.7e-46, identities = 241/244 Medline entries No Medline entry Peptide information for frame 3 ORF from 315 bp to 2027 bp; peptide length: 571 Category: strong similarity to known protein 1 MTRAGDHNRQ RGCCGSLADY LTSAKFLLYL GHSLSTWGDR MWHFAVSVFL 51 VELYGNSLLL TAVYGLVVAG SVLVLGAIIG DWVDKNARLK VAQTSLVVQN 101 VSVILCGIIL MMVFLHKHEL LTMYHGWVLT SCYILIITIA NIANLASTAT 151 AITIQRDWIV VVAGEDRSKL ANMNATIRRI DQLTNILAPM AVGQIMTFGS 201 PVIGCGFISG WNLVSMCVEY VLLWKVYQKT PALAVKAGLK EEETELKQLN 251 LHKDTEPKPL EGTHLMGVKD SNIHELEHEQ EPTCASQMAE PFRTFRDGWV 301 SYYNQPVFLA GMGLAFLYMT VLGFDCITTG YAYTQGLSGS ILSILMGASA 351 ITGIMGTVAF TWLRRKCGLV RTGLISGLAQ LSCLILCVIS VFMPGSPLDL 401 SVSPFEDIRS RFIQGESITP TKIPEITTEI YMSNGSNSAN IVPETSPESV 451 PIISVSLLFA GVIAARIGLW SFDLTVTQLL QENVIESERG IINGVQNSMN 501 YLLDLLHFIM VILAPNPEAF GLLVLISVSF VAMGHIMYFR FAQNTLGNKL 551 FACGPDAKEV RKENQANTSV V BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphutel_24j 6, frame 3 TREMBLNEW :U76714_1 gene: "CARl"; product: "cell adhesion regulator"; Rattus norvegicus cell adhesion regulator (CARl) mRNA, complete eds., N = 1, Score = 1472, P = 7.2e-151 TREMBL:AC004683_5 gene: "T19C21.5"; Arabidopsis thaliana chromosome II BAC T19C21 genomic sequence, complete sequence., N = 2, Score = 437, P = 2.8e-60 TREMBL: AF039046_2 gene: "R09B5.4"; Caenorhabditis elegans cosmid R09B5., N = 2, Score = 323, P = 1.5e-43 >TREMBLNEW:U76714_1 gene: "CARl"; product: "cell adhesion regulator"; Rattus norvegicus cell adhesion regulator (CARl) mRNA, complete eds. Length = 405 HSPs: Score = 1472 (220.9 bits), Expect = 7.2e-151, P = 7.2e-151 Identities = 288/319 (90%), Positives = 297/319 (93%) Query: 1 MTRAGDHNRQRGCCGSLADYLTSAKFLLYLGHSLSTWGDRMWHFAVSVFLVELYGNSLLL 60 MT++ D Q GCCGΞLA+YLTSAKFLLYLGHSLSTWGDRMWHFAVSVFLVELYGNSLLL Sbjct: 1 MTKSRDQTHQEGCCGΞLANYLTSAKFLLYLGHSLSTWGDRMWHFAVSVFLVELYGNSLLL 60 Query: 61 TAVYGLVVAGSVLVLGAIIGDWVDKNARLKVAQTSLVVQNVSVILCGIILMMVFLHKHEL 120 TAVYGLVVAGSVLVLGAIIGDWVDKNARLKVAQTSLVVQNVSVILCGIILMMVFLHK+EL Sbjct: 61 TAVYGLVVAGSVLVLGAIIGDWVDKNARLKVAQTSLVVQNVSVILCGIILMMVFLHKNEL 120 Query: 121 LTMYHGWVLTSCYILIITIANIANLASTATAITIQRDWIVVVAGEDRSKLANMNATIRRI 180 L MYHGWVLT CYILIITIANIANLASTATAITIQRDWIVVVAGE+RS+LA+MNATIRRI Sbjct: 121 LNMYHGWVLTVCYILIITIANIANLASTATAITIQRDWIVVVAGENRSRLADMNATIRRI 180 Query: 181 DQLTNILAPMAVGQIMTFGSPVIGCGFISGWNLVSMCVEYVLLWKVYQKTPALAVKAGLK 240 DQLTNILAPMAVGQIMTFGSPVIGCGFIΞGWNLVSMCVEY LLWKVYQKTPALAVKA LK Sbjct: 181 DQLTNILAPMAVGQIMTFGSPVIGCGFISGWNLVSMCVEYFLLWKVYQKTPALAVKAALK 240 Query: 241 EEETELKQLNLHKDTEPKPLEGTHLMGVKDSNIHELEHEQEPTCASQMAEPFRTFRDGWV 300 EE+ELKQL KDTEPKPLEGTHLMG KDSNI ELE EQEPTCASQ+AEPFRTFRDGWV Sbjct: 241 VEESELKQLTSPKDTEPKPLEGTHLMGEKDSNIRELECEQEPTCASQIAEPFRTFRDGWV 300 Query: 301 SYYNQPVFLAGMGLAF-LY 318 SYYNQPVFL G F LY Sbjct: 301 SYYNQPVFLGWHGPGFPLY 319 Pedant information for DKFZphutel_24 6, frame 3 Report for DKFZphutel_24j 6.3 [LENGTH] 571 [MW] 62542.72 [pi] 6.08 [HOMOL] TREMBL :U76714_1 gene: "CARl"; product: "cell adhesion regulator"; Rattus norvegicus cell adhesion regulator (CARl) mRNA, complete eds. le-141 [BLOCKS] BL00341D [PROSITE] MYRISTYL 15 [PROSITE] MITOCH_CARRIER 1 [PROSITE] CK2_PHOSPHO_SITE 6 [PROSITE] PROKAR_LIPOPROTEIN 1 [PROSITE] PKC_PHOSPHO_SITE 4 [PROSITE] ASN_GLYCOSYLATION 4 [PFAM] Lammm B (Domain IV) [KW] TRANSMEMBRANE 4 [KW] LOW COMPLEXITY 8.76 % SEQ MTRAGDHNRQRGCCGSLADYLTSAKFLLYLGHSLSTWGDRMWHFAVSVFLVELYGNSLLL SEG PRD ccccccccccccccccchhhhhhhheeeeccceeecccchhhhhhhhheeeeecccccee MEM MMMMMMMMMMMMM SEQ TAVYGLVVAGSVLVLGAIIGDWVDKNARLKVAQTSLVVQNVSVILCGIILMMVFLHKHEL SEG . xxxxxxxxxxxxxxxx PRD ehhhhhhhccceeeeccccccchhhhhhhhhhhhheeeccchhhhhhhhhhhhhhhhhhh MEM MMMMMMMMMMMMMMMM MMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMM SEQ LTMYHGWVLTSCYILIITIANIANLASTATAITIQRDWIVVVAGEDRSKLANMNATIRRI SEG xxxxxxxxxxxxxxxxxxxxx PRD hhcccccchhhhhhhhhhhhhhhhhhhhhheeeeccceeeeeeccccchhhhhhhhhhhh MEM MMMMMMM SEQ DQLTNILAPMAVGQIMTFGSPVIGCGFISGWNLVSMCVEYVLLWKVYQKTPALAVKAGLK SEG PRD hhhhhhccceeeceeeeeecceeeeeeeeccchhhhhhhhhhhhhhhcccchhhhhhhhh MEM SEQ EEETELKQLNLHKDTEPKPLEGTHLMGVKDSNIHELEHEQEPTCASQMAEPFRTFRDGWV SEG PRD hhhhhhhhhhccccccccccceeeeeecccccccccccccccccccccccccccccccee MEM SEQ SYYNQPVFLAGMGLAFLYMTVLGFDCITTGYAYTQGLSGSILSILMGASAITGIMGTVAF SEG PRD eeecceeeecccchhhhhhcccccceeeeeeeeccccceeeeeeecccceeeeehhhhhh MEM SEQ TWLRRKCGLVRTGLISGLAQLSCLILCVISVFMPGSPLDLSVSPFEDIRSRFIQGESITP SEG XXX PRD hhhhhhccccccccchhhhhhhhhhhhhhhhcccccccccccccchhhhhhccccccccc MEM SEQ TKIPEITTEIYMSNGSNΞANIVPETSPESVPIISVSLLFAGVIAARIGLWΞFDLTVTQLL SEG xxxxxxxxxx PRD ccccccceeeeecccccccccccccccccceeeeeehhhhhhhhhhcccchhhhhhhhhh MEM MMMMMMMMMMMMMMMMMMMMMMMM SEQ QENVIEΞERGIINGVQNΞMNYLLDLLHFIMVILAPNPEAFGLLVLISVSFVAMGHIMYFR SEG PRD hhhhhccccceeeecccchhhhhhhhhhheeeeeccccccceeeeeeeeccccccceeee MEM MMMMMMMMMMMMMMMMMMMMMMMMMMM ... SEQ FAQNTLGNKLFACGPDAKEVRKENQANTSVV SEG PRD eecccccceeeeccccchhhhhhhhcccccc MEM Prosite for DKFZphutel_24j 6.3 PS00001 100->104 ASN_GLYCOSYLATION PDOC00001 PS00001 174->178 ASN_GLYCOSYLATION PDOC00001 PS00001 434->438 ASN_GLYCOSYLATION PDOC00001 PS00001 567->571 ASN_GLYCOSYLATION PDOC00001 PS00005 23->26 PKC_PHOSPHO_SITE PDOC00005 PS00005 176->179 PKC_PHOSPHO_SITE PDOC00005 PS00005 294->297 PKC_PHOSPHO_SITE PDOC00005 PS00005 487->490 PKC_PHOSPHO_SITE PDOC00005 PS00006 16->20 CK2_PHOSPHO_SITE PDOC00006 PS00006 36->40 CK2_PHOSPHO_SITE PDOC00006 PS00006 294->298 CK2_PHOSPHO_SITE PDOC00006 PS00006 396->400 CK2_PHOSPHO_SITE PDOC00006 PS00006 403->407 CK2_PHOSPHO_SITE PDOC00006 PS00006 445->449 CK2_PHOSPHO_SITE PDOC00006 PS00008 12->18 MYRISTYL PDOC00008 PS00008 65->71 MYRISTYL PDOC00008 PS00008 76->82 MYRISTYL PDOC00008 PS00008 193->199 MYRISTYL PDOC00008 PS00008 267->273 MYRISTYL PDOC00008 PS00008 311->317 MYRISTYL PDOC00008 PS00008 336->342 MYRISTYL PDOC00008 PS00008 339->345 MYRISTYL PDOC00008 PS00008 353->359 MYRISTYL PDOC00008 PS00008 368->374 MYRISTYL PDOC00008 PS00008 373->379 MYRISTYL PDOC00008 PS00008 435->441 MYRISTYL PDOC00008 PS00008 461->467 MYRISTYL PDOC00008 PS00008 490->496 MYRISTYL PDOC00008 PS00008 494->500 MYRISTYL PDOC00008 PS00013 122->133 PROKAR_LIPOPROTEIN PDOC00013 PS00215 404->414 MITOCH CARRIER PDOC00189 Pfam for DKFZphutel_24j 6.3 HMM_NAME Lammm B (Domain IV) HMM *YWRlPERFLGDQvTsYGGkLe* Y+R + LG+++ + G + + Query 538 YFRFAQNTLGNKLFACGPDAK 558 DKFZphutel_2h3 group: differentiation/development DKFZphutel_2h3 encodes a novel 267 amino acid protein, with similarity to ITM2 (integral membrane protein 2) of chicken and mouse. The novel protein contains a prenyl group binding site (CAAX box) and seems to be post- translationally modified by the attachment of either a farnesyl or a geranyl-geranyl group. The similar gallus G. protein E25 a marker for chondro-osteogemc differentiation. The new protein can find application as a useful marker for chondro-osteogemc cell differentiation and for the modulation of chondro-osteogemc cell differentiation. strong similarity to mouse E25 and gallus E3-16 complete cDNA, EST hits complete eds according to E25 start at Bp 56 putative transmembrane protein (1 TM) Sequenced by AGOWA Locus : unknown Insert length: 2033 bp Poly A stretch at pos. 2007, polyadenylation signal at pos. 1986 1 GGACCGAGGC TGCACCGGCA GAGGCTGCGG GGCGGACGCG CGGGCCGGCG 51 CAGCCATGGT GAAGATTAGC TTCCAGCCCG CCGTGGCTGG CATCAAGGGC 101 GACAAGGCTG ACAAGGCGTC GGCGTCGGCC CCTGCGCCGG CCTCGGCCAC 151 CGAGATCCTG CTGACGCCGG CTAGGGAGGA GCAGCCCCCA CAACATCGAT 201 CCAAGAGGGG GAGCTCAGTG GGCGGCGTGT GCTACCTGTC GATGGGCATG 251 GTCGTGCTGC TCATGGGCCT CGTGTTCGCC TCTGTCTACA TCTACAGATA 301 CTTCTTTCTT GCACAGCTGG CCCGAGATAA CTTCTTCCGC TGTGGTGTGC 351 TGTATGAGGA CTCCCTGTCC TCCCAGGTCC GGACTCAGAT GGAGCTGGAA 401 GAGGATGTGA AAATCTACCT CGACGAGAAC TACGAGCGCA TCAACGTGCC 451 TGTGCCCCAG TTTGGCGGCG GTGACCCTGC AGACATCATC CATGACTTCC 501 AGCGGGGTCT GACTGCGTAC CATGATATCT CCCTGGACAA GTGCTATGTC 551 ATCGAACTCA ACACCACCAT TGTGCTGCCC CCTCGCAACT TCTGGGAGCT 601 CCTCATGAAC GTGAAGAGGG GGACCTACCT GCCGCAGACG TACATCATCC 651 AGGAGGAGAT GGTGGTCACG GAGCATGTCA GTGACAAGGA GGCCCTGGGG 701 TCCTTCATCT ACCACCTGTG CAACGGGAAA GACACCTACC GGCTCCGGCG 751 CCGGGCAACG CGGAGGCGGA TCAACAAGCG TGGGGCCAAG AACTGCAATG 801 CCATCCGCCA CTTCGAGAAC ACCTTCGTGG TGGAGACGCT CATCTGCGGG 851 GTGGTGTGAG GCCCTCCTCC CCCAGAACCC CCTGCCGTGT TCCTCTTTTC 901 TTCTTTCCAG CTGCTCTCTG GCCCTCCTCC TTCCCCCTGC TTAGCTTGTA 951 CTTTGGACGC GTTTCTATAG AGGTGACATG TCTCTCCATT CCTCTCCAAC 1001 CCTGCCCACC TCCCTGTACC AGAGCTGTGA TCTCTCGGTG GGGGGCCCAT 1051 CTCTGCTGAC CTGGGTGTGG CGGAGGGAGA GGCGATGCTG CAAAGTGTTT 1101 TCTGTGTCCC ACTGTCTTGA AGCTGGGCCT GCCAAAGCCT GGGCCCACAG 1151 CTGCACCGGC AGCCCAAGGG GAAGGACCGG TTGGGGGAGC CGGGCATGTG 1201 AGGCCCTGGG CAAGGGGATG GGGCTGTGGG GGCGGGGCGG CATGGGCTTC 1251 AGAAGTATCT GCACAATTAG AAAAGTCCTC AGAAGCTTTT TCTTGGAGGG 1301 TACACTTTCT TCACTGTCCC TATTCCTAGA CCTGGGGCTT GAGCTGAGGA 1351 TGGGACGATG TGCCCAGGGA GGGACCCACC AGAGCACAAG AGAAGGTGGC 1401 TACCTGGGGG TGTCCCAGGG ACTCTGTCAG TGCCTTCAGC CCACCAGCAG 1451 GAGCTTGGAG TTTGGGGAGT GGGGATGAGT CCGTCAAGCA CAACTGTTCT 1501 CTGAGTGGAA CCAAAGAAGC AAGGAGCTAG GACCCCCAGT CCTGCCCCCC 1551 AGGAGCACAA GCAGGGTCCC CTCAGTCAAG GCAGTGGGAT GGGCGGCTGA 1601 GGAACGGGGC AGGCAAGGTC ACTGCTCAGT CACGTCCACG GGGGACGAGC 1651 CGTGGGTTCT GCTGAGTAGG TGGAGCTCAT TGCTTTCTCC AAGCTTGGAA 1701 CTGTTTTGAA AGATAACACA GAGGGAAAGG GAGAGCCACC TGGTACTTGT 1751 CCACCCTGCC TCCTCTGTTC TGAAATTCCA TCCCCCTCAG CTTAGGGGAA 1801 TGCACCTTTT TCCCTTTCCT TCTCACTTTT GCATGTTTTT ACTGATCATT 1851 CGATATGCTA ACCGTTCTCA GCCCTGAGCC TTGGAGAGGA GGGCTGTAAC 1901 GCCTTCAGTC AGTCTCTGGG GATGAAACTC TTAAATGCTT TGTATATTTT 1951 CTCAATTAGA TCTCTTTTCA GAAGTGTCTA TAGAACAATA AAAATCTTTT 2001 ACTTCTGAAA AAAAAAAAAA AAAAGGGCGG CCG BLAST Results Entry B64417 from database EMBL: CIT-HSP-2023A7.TR CIT-HSP Homo sapiens genomic clone 2023A7. Length = 715 Plus Strand HSPs : Score = 1546 (232.0 bits), Expect = 7.8e-64, P = 7.8e-64 Identities = 310/311 (99%) Medline entries 96325063: Isolation of markers for chondro-osteogemc differentiation using cDNA library subtraction. Molecular cloning and characterization of a gene belonging to a novel multigene family of integral membrane proteins . Peptide information for frame 2 ORF from 56 bp to 856 bp; peptide length: 267 Category: strong similarity to known protein 1 MVKISFQPAV AGIKGDKADK ASASAPAPAS ATEILLTPAR EEQPPQHRSK 51 RGSSVGGVCY LSMGMVVLLM GLVFASVYIY RYFFLAQLAR DNFFRCGVLY 101 EDSLSSQVRT QMELEEDVKI YLDENYERIN VPVPQFGGGD PADIIHDFQR 151 GLTAYHDISL DKCYVIELNT TIVLPPRNFW ELLMNVKRGT YLPQTYIIQE 201 EMVVTEHVSD KEALGSFIYH LCNGKDTYRL RRRATRRRIN KRGAKNCNAI 251 RHFENTFVVE TLICGVV BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphutel_2h3, frame 2 SWISSNEW:ITMB_CHICK INTEGRAL MEMBRANE PROTEIN 2B (TRANSMEMBRANE PROTEIN E3-16)., N = 1, Score = 573, P = 1.3e-55 SWISSNEW:ITMB_MOUSE INTEGRAL MEMBRANE PROTEIN 2B (E25B PROTEIN)., N = 1, Score = 560, P = 3.2e-54 SWISSNEW:ITMA_HUMAN INTEGRAL MEMBRANE PROTEIN 2A (E25 PROTEIN)., N = 1, Score = 456, P = 3.3e-43 >SWISSNEW:ITMB_CHICK INTEGRAL MEMBRANE PROTEIN 2B (TRANSMEMBRANE PROTEIN E3-16) . Length = 262 HSPs: Score = 573 (86.0 bits), Expect = 1.3e-55, P = 1.3e-55 Identities = 117/264 (44%), Positives = 172/264 (65%) Query: 1 MVKISFQPAVAGIKGDKADKASASAPAPASATEILLTPAREEQPPQHRSKRGSSVGGVCY 60 MVK+SF A+A + A+K ++ ++L+ P ++P G Sbjct: 1 MVKVSFNSALA—HKEAANKEEENS QVLILPPDAKEPEDVVVPAGHKRAWCWC 51 Query: 61 LSMGMVVLLMGLVFASVYIYRYFFLAQLARDNFFRCGVLY-EDSLS ΞQVRTQM-- 112 + G+ +L G++ Y+Y+YF Q + CG+ Y ED LS +Q+++ Sbjct: 52 MCFGLAFMLAGVILGGAYLYKYFAFQQ GGVYFCGIKYIEDGLSLPESGAQLKSARYH 108 Query: 113 ELEEDVKIYLDENYERINVPVPQFGGGDPADIIHDFQRGLTAYHDISLDKCYVIELNTTI 172 +E++++I +E+ E I+VPVP+F DPADI+HDF R LTAY D+SLDKCYVI LNT++ Sbjct: 109 TIEQNIQILEEEDVEFISVPVPEFADSDPADIVHDFHRRLTAYLDLSLDKCYVIPLNTSV 168 Query: 173 VLPPRNFWELLMNVKRGTYLPQTYIIQEEMVVTEHVSDKEALGSFIYHLCNGKDTYRLRR 232 V+PP+NF ELL+N+K GTYLPQ+Y+I E+M+VT+ + + + LG FIY LC GK+TY+L+R Sbjct: 169 VMPPKNFLELLINIKAGTYLPQSYLIHEQMIVTDRIENVDQLGFFIYRLCRGKETYKLQR 228 Query: 233 RATRRRINKRGAKNCNAIRHFENTFVVETLIC 264 + + I KR A NC IRHFEN F +ETLIC Sbjct: 229 KEAMKGIQKREAVNCRKIRHFENRFAMETLIC 260 Pedant information for DKFZphutel_2h3, frame 2 Report for DKFZphutel_2h3.2 [LENGTH] 267 [MW] 30253.96 [pi] 8.16 [HOMOL] SWISSNEW:ITMB_CHICK INTEGRAL MEMBRANE PROTEIN 2B (TRANSMEMBRANE PROTEIN E3-16) le-49 [PROSITE] MYRISTYL 4 [PROSITE) PRENYLATION 1 [PROSITE] CAMP_PHOSPHO_ΞITE 3 [PROSITE] CK2_PHOSPHO_SITE 3 [PROSITE] TYR_PHOSPHO_SITE 1 [PROSITE] PKC_PHOSPHO_SITE 4 [PROSITE] ASN_GLYCOSYLATION 1 [KW] TRANSMEMBRANE 1 [KW] LOW COMPLEXITY 15.36 % SEQ MVKISFQPAVAGIKGDKADKASASAPAPASATEILLTPAREEQPPQHRSKRGSSVGGVCY SEG xxxxxxxxxxxxxxxx PRD ccccccccchhhhhhhhhhhhhhhhhccccccceeecccccccccccccccccccccchh MEM MMMM SEQ LSMGMVVLLMGLVFASVYIYRYFFLAQLARDNFFRCGVLYEDSLSSQVRTQMELEEDVKI SEG .. xxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhcchhhhhhhhhhccceeeeeecccccccchhhhhhhhhhhhh MEM MMMMMMMMMMMMMMMMMMMMMMMM SEQ YLDENYERINVPVPQFGGGDPADIIHDFQRGLTAYHDISLDKCYVIELNTTIVLPPRNFW SEG PRD hhcccceeeeccccccccccccchhhhhhhhhhhhhhhcccceeeeeccceeecccchhh MEM SEQ ELLMNVKRGTYLPQTYIIQEEMVVTEHVSDKEALGSFIYHLCNGKDTYRLRRRATRRRIN SEG xxxxxxxxxxxx PRD hhhhhhcccccccceeeeehhhhhhhccccchhhhhheeeccccchhhhhhhhhhhhhhh MEM SEQ KRGAKNCNAIRHFENTFVVETLICGVV SEG xx PRD hhhhccceeeecccchhhhhheeeccc MEM Prosite for DKFZphutel_2h3.2 PS00001 169->173 ASN_GLYCOSYLATION PDOC00001 PS00004 50->54 CAMP_PHOSPHO_SITE PDOC00004 PS00004 187-M91 CAMP_PHOSPHO_SITE PDOC00004 PS00004 232->236 CAMP_PHOSPHO_SITE PDOC00004 PS00005 49->52 PKC_PHOSPHO_SITE PDOC00005 PS00005 209->212 PKC_PHOSPHO_SITE PDOC00005 PS00005 227->230 PKC_PHOSPHO_SITE PDOC00005 PS00005 235->238 PKC_PHOSPHO_SITE PDOC00005 PS00006 30->34 CK2_PHOSPHO_SITE PDOC00006 PS00006 110->114 CK2_PHOSPHO_SITE PDOC00006 PS00006 209->213 CK2_PHOSPHO_SITE PDOC00006 PS00007 119->127 TYR_PHOSPHO_SITE PDOC00007 PS00008 52->58 MYRISTYL PDOC00008 PS00008 71->77 MYRISTYL PDOC00008 PS00008 138->144 MYRISTYL PDOC00008 PS00008 243->249 MYRISTYL PDOC00008 PS00294 264->268 PRENYLATION PDOC00266 (No Pfam data available for DKFZphutel 2h3.2) DKFZphmcfl lall group: transmembrane protein DKFZphmcfl_lall encodes a novel 393 ammo acid protein with weak similarity to S. pombe SPBC29A3_3 protein and S. cerevisiae putative membrane protein YDR255c. The novel protein contains 1 transmembrane region. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of mammary carcinoma- specific genes and as a new marker for mammary carcinoma cells. similarity to YDR255c and SPBC29A3.03c membrane regions : 1 Summary DKFZphmcfl_lall encodes a novel 393 amino acid protein, with similarity to YDR255c and SPBC29A3.03c . similarity to YDR255C and SPBC29A3.03c complete cDNA, complete eds, EST hits potential start at Bp 110 matches kozak consensus Sequenced by DKFZ Locus: /map="542.7 cR from top of Chr5 linkage group" Insert length: 1819 bp Poly A stretch at pos. 1808, no polyadenylation signal found 1 CCCGGCCCAG CCCCCGAAGA GCCGCCTCAG CCGGGGGGAG TTGCTCGGAC 51 TCAAACGTCC AGTCCTCGTG CGACCGCGCT GGGTCGGAAG TGAGCAGGCT 101 GAGGCCACCA TGGAGCAGTG TGCGTGCGTG GAGAGAGAGC TGGACAAGGT 151 CCTGCAGAAG TTCCTGACCT ACGGGCAGCA CTGTGAGCGG AGCCTGGAGG 201 AGCTGCTGCA CTACGTGGGC CAGCTGCGGG CTGAGCTGGC CAGCGCAGCC 251 CTCCAGGGGA CCCCTCTCTC AGCCACCCTC TCTCTGGTGA TGTCACAGTG 301 CTGCCGGAAG ATCAAAGATA CGGTGCAGAA ACTGGCTTCG GACCATAAGG 351 ACATTCACAG CAGTGTATCC CGAGTGGGCA AAGCCATTGA CAGGAACTTC 401 GACTCTGAGA TCTGTGGTGT TGTGTCAGAT GCGGTGTGGG ACGCGCGGGA 451 ACAGCAGCAG CAGATCCTGC AGATGGCCAT CGTGGAACAC CTGTATCAGC 501 AGGGCATGCT CAGCGTGGCC GAGGAGCTGT GCCAGGAATC AACGCTGAAT 551 GTGGACTTGG ATTTCAAGCA GCCTTTCCTA GAGTTGAATC GAATCCTGGA 601 AGCCCTGCAC GAACAAGACC TGGGTCCTGC GTTGGAATGG GCCGTCTCCC 651 ACAGGCAGCG CCTGCTGGAA CTCAACAGCT CCCTGGAGTT CAAGCTGCAC 701 CGACTGCACT TCATCCGCCT CTTGGCAGGA GGCCCCGCGA AGCAGCTGGA 751 GGCCCTCAGC TATGCTCGGC ACTTCCAGCC CTTTGCTCGG CTGCACCAGC 801 GGGAGATCCA GGTGATGATG GGCAGCCTGG TGTACCTGCG GCTGGGCTTG 851 GAGAAGTCAC CCTACTGCCA CCTGCTGGAC AGCAGCCACT GGGCAGAGAT 901 CTGTGAGACC TTTACCCGGG ACGCCTGTTC CCTGCTGGGG CTTTCTGTGG 951 AGTCCCCCCT TAGCGTCAGC TTTGCCTCTG GCTGTGTGGC GCTGCCTGTG 1001 TTGATGAACA TCAAGGCTGT GATTGAGCAG CGGCAGTGCA CTGGGGTCTG 1051 GAATCACAAG GACGAGTTAC CGATTGAGAT TGAACTAGGC ATGAAGTGCT 1101 GGTACCACTC CGTGTTCGCT TGCCCCATCC TCCGCCAGCA GACGTCAGAT 1151 TCCAACCCTC CCATCAAGCT CATCTGTGGC CATGTTATCT CCCGAGATGC 1201 ACTCAATAAG CTCATTAATG GAGGAAAGCT GAAGTGTCCC TACTGTCCCA 1251 TGGAGCAGAA CCCGGCAGAT GGGAAACGCA TCATATTCTG ATTCCTACCT 1301 GGAAGGAATT TTGTTGAAAG GGGTTTTCAC CTGTGAGCCT TGGTCTGTCT 1351 CGGTAGGGTG GTCAACTTCA GTGGACTGTG GTTGGTTTCA GAGCGCCTGG 1401 CTGAGGAGTT CCACTGAGGG GAGCACTGGA GCAGCCCTTT GGCAGAGGCT 1451 GAGGAGGGAG ATGGACCAGC CCACGCCTGG CACCTGGCTC CATGGCATAA 1501 GGAAAGGGAG ATGCTGGCCT CTGTGCTCCT GCTGTCTTTT CCTGTTTCTG 1551 TTTGCGTTTG ACTTAGTAGC AACCGACAGA GTGGCAAGGG ATTTGGTCTT 1601 CAGCAGTAGA CATCCTTCCA CCCCTGCCCT CAGCCAAGTC TCTTGCTGCC 1651 ATGCCAATGC TATGTCCACC CTTGCCCCTC GGCCCAAGAG TGTCCAGCGG 1701 TGGCCCACCT CTTCCTCCCA CTACAGCCTC AACAGTATGT ACCATCTCCC 1751 ACTGTAAATA GTCCCAGTTA GAACGGAATG CCGTTGTTTT ATAACTTTGA 1801 ACAAATGTAA AAAAAAAAA BLAST Results Entry HS579359 from database EMBL: human STS WI-6350. Score = 1027, P = 9.9e-40, identities = 207/209 Medline entries No Medline entry Peptide information for frame 2 ORF from 110 bp to 1288 bp; peptide length: 393 Category: similarity to unknown protein 1 MEQCACVERE LDKVLQKFLT YGQHCERSLE ELLHYVGQLR AELASAALQG 51 TPLSATLSLV MSQCCRKIKD TVQKLASDHK DIHSSVSRVG KAIDRNFDSE 101 ICGVVSDAVW DAREQQQQIL QMAIVEHLYQ QGMLSVAEEL CQESTLNVDL 151 DFKQPFLELN RILEALHEQD LGPALEWAVS HRQRLLELNS ΞLEFKLHRLH 201 FIRLLAGGPA KQLEALSYAR HFQPFARLHQ REIQVMMGSL VYLRLGLEKS 251 PYCHLLDSSH WAEICETFTR DACSLLGLSV ESPLSVSFAS GCVALPVLMN 301 IKAVIEQRQC TGVWNHKDEL PIEIELGMKC WYHSVFACPI LRQQTSDSNP 351 PIKLICGHVI SRDALNKLIN GGKLKCPYCP MEQNPADGKR IIF BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphmcfl_lall , frame 2 TREMBL: SPBC29A3_3 gene: "SPBC29A3.03c"; product: "hypothetical protein"; S. pombe chromosome II cosmid c29A3., N = 2, Score = 302, P = 3.4e-42 PIR:S67312 probable membrane protein YDR255c - yeast (Saccharomyces cerevisiae), N = 1, Score = 271, P = 5.3e-22 TREMBL :CET07D1_2 gene: "T07D1.2"; Caenorhabditis elegans cosmid T07D1., N = 1, Score = 193, P = 5.6e-13 >TREMBL:SPBC29A3_3 gene: "SPBC29A3.03c"; product: "hypothetical protein"; S. pombe chromosome II cosmid c29A3. Length = 398 HSPs: Score = 302 (45.3 bits), Expect = 3.4e-42, Sum P(2) = 3.4e-42 Identities = 55/142 (38%), Positives = 89/142 (62%) Query: 252 YCHLLDSSHWAEICETFTRDACSLLGLSVESPLSVSFASGCVALPVLMNIKAVIEQRQCT 311 Y +LD W + F R+ C+ LG+S+ESPL + +G +ALP+L+ + ++++++ Sbjct: 258 YIDVLDLD-WKSLELLFVREFCAALGMSLESPLDIVVNAGAIALPILLKMSSIMKKKHTE 316 Query: 312 GVWNHKDELPIEIELGMKCWYHSVFACPILRQQTSDSNPPIKLICGHVISRDALNKLING 371 W + ELP+EI L +HSVF CP+ ++Q ++ NPP+ + CGHVI +++L +L Sbjct: 317 —WTSQGELPVEIFLPSSYHFHSVFTCPVSKEQATEENPPMMMSCGHVIVKESLRQLΞRN 374 Query: 372 G—KLKCPYCPMEQNPADGKRIIF 393 G + KCPYCP E AD R+ F Sbjct: 375 GSQRFKCPYCPNENVAADAIRVYF 398 Score = 161 (24.2 bits), Expect = 3.4e-42, Sum P(2) = 3.4e-42 Identities = 51/221 (23%), Positives = 102/221 (46%) Query: 22 GQHCERSLEELLHYVGQLRAELASAALQGTPLSATLSLVMSQCCRKIKDTVQKLASDHKD 81 G C L EL + + + L+ P ++ LV C K + L K Sbjct: 15 GNKCLAKLNEL ESILKDAKKSCLKD-PTTSMKELVA—CSEKTQQVFDDLKRTEKK 67 Query: 82 IHSSVSRVGKAIDRNFDΞEICGVVSDAVWDAREQQQQILQMAIVEHLYQQGMLSVAEELC 141 H+S++R GK +++ F+ ++ + + +++++++ + A+ H ++QG + +A C Sbjct: 68 FHTSLNRFGKTLEKKFNFDLEDIKLHSSFESKKRE IDTALSLHFFRQGDVELAHLFC 124 Query: 142 QESTLNVDLDFKQPFLELNRILEALHEQDLGPALEWAVSHRQRLLELNSSLEFKLHRLHF 201 +E+ + + F L I++ + ++DL +EWA R L SSLE+ L + Sbjct: 125 KEAGIEEPSESLHVFTLLKSIVQGIRDKDLKLPIEWASQCRGYLERKGSSLEYTLQKYRL 184 Query: 202 IRLLAGGPAKQL-EALSYAR-HFQPFARLHQREIQVMMGSLVY 242 + K + A+ Y R + F + H +IQ M +L + Sbjct: 185 VSNYL—TTKDIMAAIRYCRTNMAEFQKKHLADIQKTMIALFF 225 Pedant information for DKFZphmcfl_lall, frame 2 Report for DKFZphmcfl_lall .2 LENGTH] 393 MW] 44414.77 pi] 6.15 HOMOL] TREMBL :SPBC29A3 3 gene: "SPBC29A3.03c"; product: "hypothetical protein" pombe chromosome II cosmid c29A3. 2e-39 FUNCAT] 99 unclassified proteins [S. cerevisiae, YDR255c] 8e-23 PIRKW] transmembrane protein 2e-21 PROSITE] MYRISTYL 2 PROSITE] AMIDATION 1 PROSITE] CK2_PHOSPHO_SITE 3 PROSITE] PROKAR_LIPOPROTEIN 1 PROSITE] TYR_PHOSPHO_SITE 3 PROSITE] PKC_PHOSPHO_SITE 1 PROSITE] ASN_GLYCOSYLATION 1 KW] TRANSMEMBRANE 1 SEQ MEQCACVERELDKVLQKFLTYGQHCERSLEELLHYVGQLRAELASAALQGTPLSATLSLV PRD ccceeehhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccchhhhh MEM SEQ MSQCCRKIKDTVQKLASDHKDIHSSVSRVGKAIDRNFDSEICGVVSDAVWDAREQQQQIL PRD hhhhhhhhhhhhhhhhhhhhhhcccccccchhhhhccccceeeechhhhhhhhhhhhhhh MEM SEQ QMAIVEHLYQQGMLSVAEELCQESTLNVDLDFKQPFLELNRILEALHEQDLGPALEWAVS PRD hhhhhhhhhhhccchhhhhhhhhhhccccccccchhhhhhhhhhhhhhccccchhhhhhh MEM SEQ HRQRLLELNSSLEFKLHRLHFIRLLAGGPAKQLEALSYARHFQPFARLHQREIQVMMGSL PRD hhhhhhhcccchhhhhhhhhhhhhhccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhh MEM SEQ VYLRLGLEKSPYCHLLDSSHWAEICETFTRDACSLLGLSVESPLSVSFASGCVALPVLMN PRD hhcccccccccccccccchhhhhhhhhhhhhhhhhhhhcccccceeeecccccchhhhhh MEM MMMMMMMMMMMMMMMMMMMMMMM SEQ IKAVIEQRQCTGVWNHKDELPIEIELGMKCWYHSVFACPILRQQTSDSNPPIKLICGHVI PRD hhhhhhhhhhhcccccccccceeeeeccceeeeeeeecchhhhhccccccccccccceee MEM MMMMMM SEQ SRDALNKLINGGKLKCPYCPMEQNPADGKRIIF PRD eehhhhhhhccccccccccccccchhhhhcccc MEM Prosite for DKFZphmcfl_lall .2 PS00001 189->193 ASN_GLYCOSYLATION PDOC00001 PΞ00005 180->183 PKC_PHOSPHO_SITE PDOC00005 PS00006 28->32 CK2_PHOSPHO_SITE PDOC00006 PS00006 135->139 CK2_PHOSPHO_SITE PDOC00006 PS00006 190->194 CK2_PHOSPHO_SITE PDOC00006 PS00007 211->219 TYR_PHOSPHO_SITE PDOC00007 PS00007 27->36 TYR_PHOSPHO_SITE PDOC00007 PS00007 244->253 TYR_PHOSPHO_SITE PDOC00007 PS00008 37->43 MYRISTYL PDOC00008 PS00008 50->56 MYRISTYL PDOC00008 PS00009 387->391 AMIDATION PDOC00009 PS00013 282->293 PROKAR LIPOPROTEIN PDOC00013 (No Pfam data available for DKFZphmcfl_lall .2) DKFZphmcfl_lc23 group: mammary carcinoma derived DKFZphmcfl_lc23.1 encodes a novel 311 amino acid proline rich protein. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application m studying the expression profile of mamma carcinoma- specific genes. unknown, proline rich protein complete cDNA, complete eds? potential start at Bp 50, EST hits Sequenced by DKFZ Locus: unknown Insert length: 3077 bp Poly A stretch at pos. 3067, polyadenylation signal at pos. 3048 1 AACTGGCCCC CTCCCCCACC CCCTGCCCCT GAGGAGCAGG ACCTGTCCAT 51 GGCTGACTTC CCCCCACCAG AGGAGGCTTT TTTCTCTGTG GCCAGCCCTG 101 AGCCTGCAGG CCCTTCAGGC TCCCCAGAGC TTGTCAGCTC CCCGGCTGCT 151 TCGTCCTCCT CAGCTACTGC TTTGCAGATT CAGCCCCCGG GTAGCCCAGA 201 CCCTCCTCCA GCTCCGCCAG CCCCAGCTCC TGCTAGTTCC GCCCCAGGGC 251 ATGTGGCCAA GCTCCCTCAG AAGGAACCGG TGGGCTGTAG CAAGGGTGGT 301 GGGCCTCCCA GGGAGGACGT AGGTGCGCCC CTGGTCACGC CCTCGCTCCT 351 GCAGATGGTG CGGCTGCGCT CCGTGGGTGC TCCAGGAGGG GCTCCCACCC 401 CAGCACTGGG GCCATCGGCC CCCCAGAAAC CACTGCGAAG GGCCCTGTCA 451 GGGCGGGCCA GCCCAGTGCC TGCCCCCTCC TCAGGGCTCC ATGCTGCGGT 501 CCGACTCAAG GCCTGCAGCC TGGCCGCCAG TGAAGGCCTC TCAAGTGCTC 551 AGCCCAACGG ACCGCCTGAG GCAGAGCCAC GGCCTCCCCA GTCCCCTGCC 601 TCAACGGCCA GTTTCATCTT CTCCAAGGGC TCTAGGAAGC TGCAGCTGGA 651 GCGGCCCGTG TCCCCTGAGA CCCAGGCTGA CCTCCAGCGG AATCTGGTGG 701 CAGAACTCCG GAGCATCTCA GAGCAGCGGC CACCCCAGGC CCCAAAGAAG 751 TCACCTAAGG CTCCCCCACC TGTGGCCCGC AAGCCGTCTG TGGGAGTCCC 801 CCCACCCGCC TCCCCCAGTT ACCCTCGAGC TGAGCCCCTT ACTGCTCCTC 851 CCACCAATGG GCTCCCTCAC ACCCAGGACA GGACTAAGAG GGAGCTGGCG 901 GAGAATGGAG GTGTCCTGCA GCTGGTGGGC CCAGAGGAGA AGATGGGCCT 951 CCCGGGCTCA GACTCACAGA AAGAGCTGGC CTGACCACCA GGCACCTCAC 1001 TGGCACTGCT GACCCATCCC AGAAACACAA TCTCAGGGAC CCGAGCAGCT 1051 CCAAGGACGA GAGGATACAG CAGACACAAC CTAATAGAGA GGGCGCCTGC 1101 AGCCTTAACC TCCACGGCCT TCGATACTTA TGCAAGCCTG GTGTTGCTCC 1151 TGTCCTCAGA GTCATCCTGC GCTCATGCCT TTTCCCGAAT GGGTTCACCT 1201 CTGGCAGTTG CCGCTTCAGT CTTGGCCTTA GCCTCATCTT GAAGTGGGTA 1251 GCTGGCGGGA GAGGGTGGCT GCGCCCCCTG CTGGCCCTGA GGCTGCAGAG 1301 TTGGGAGCAG GACACCTCAC CTGAGTTTCA TTTTTTTTCA TGTCCAAACC 1351 ATGCACATAC TATAGTCCAG AATCAAAGCA CTTTTGAAAA GTGGCTGCAT 1401 GGCCATCCTC CAGGGCCCAG GAAGTTGCAT TCCAAGGGCC TGTTTACATG 1451 GCAGCAGAAT CCATCCCCGG CAGTCAGCCC ATAGCTTGGG ACCAGTCTGT 1501 GCCCTCCTGC CCAGTCCAGT TTACTCCTCT TGGTTCCTGA AGGTGGCCAA 1551 GTCATTGTGT TCCCACAGGC TTCTCTAGGC TGGGGGCAGG TGTGGGGCTG 1601 TGGAATTCCA AAGCACAAAA GGTGCAGAGG GGATTGGCCT TCCTGTGCCT 1651 CAACTCACCA ACCACCCTCC TGCCTTCCAG TTCTGCCAGG TGCTCCATGC 1701 TGGGGACAAG TAGGAGACTG CCAGGGCCCA AAGAAATGGG TGAGCAGTAG 1751 AGTCATCTCG GGGCACTTGG CAGTGTCAAG CACCTGCCCC TTGCCTCCTT 1801 GACCACACTG GGGTGGGTGG GCCCCCAGCA CTTCAGAGGC AGGAGCCTTT 1851 GGGCTGAGCA AGCACTGAGG AGGTGGATGG AAGGGAGCAT CTGGAGGGGG 1901 GGAGCTTCCT TGAGCAGTGG GCCCAGGCCT GGCCCTCCAC ACTTCATTCT 1951 CTGACCTTTC TCTCTCCTCA TTTCGGTGCA TGTCCTTTCT GCAGCTGCCT 2001 TTCAGCACAG GTGGTTCCAC TGGGGGCAGC TAACGCTGAG TGACAAGGAT 2051 GGGAAGCCAC AGGTGCATTT TACTCAAGTC TTCTCTAGTC AATGAGGGGC 2101 ACCCAGTGCT TCTAGGGCAG GCTGGGTGGT GGTCCCCTAG GTATCAGCCT 2151 CTCTTACTGT ACTCTCCGGG AATGTTAACC TTTCTATTTT CAGCCTGTGC 2201 CACCTGTCTA GGCAAGCTGG CTTCCCCATT GGCCCCTGTG GGTCCACAGC 2251 AGCGTGGCTG CCCCCCAGGG CCACCGCTTC TTTCTTGATC CTCTTTCCTT 2301 AACAGTGACT TGGGCTTGAG TCTGGCAAGG AACCTTGCTT TTAGCTTCAC 2351 CACCAAGGAG AGAGGTTGAC ATGACCTCCC CGCCCCCTCA CCAAGGCTGG 2401 GAACAGAGGG GATGTGGTGA GAGCCAGGTT CCTCTGGCCC TCTCCAGGGT 2451 GTTTTCCACT AGTCACTACT GTCTTCTCCT TGTAGCTAAT CAATCAATAT 2501 TCTTCCCTTG CCTGTGGGCA GTGGAGAGTG CTGCTGGGTG TACGCTGCAC 2551 CTGCCCACTG AGTTGGGGAA AGAGGATAAT CAGTGAGCAC TGTTCTGCTC 2601 AGAGCTCCTG ATCTACCCCA CCCCCTAGGA TCCAGGACTG GGTCAAAGCT 2651 GCATGAAACC AGGCCCTGGC AGCAACCTGG GAATGGCTGG AGGTGGGAGA 2701 GAACCTGACT TCTCTTTCCC TCTCCCTCCT CCAACATTAC TGGAACTCTA 2751 TCCTGTTAGG ATCTTCTGAG CTTGTTTCCC TGCTGGGTGG GACAGAGGAC 2801 AAAGGAGAAG GGAGGGTCTA GAAGAGGCAG CCCTTCTTTG TCCTCTGGGG 2851 TAAATGAGCT TGACCTAGAG TAAATGGAGA GACCAAAAGC CTCTGATTTT 2901 TAATTTCCAT AAAATGTTAG AAGTATATAT ATACATATAT ATATTTCTTT 2951 AAATTTTTGA GTCTTTGATA TGTCTAAAAA TCCATTCCCT CTGCCCTGAA 3001 GCCTGAGTGA GACACATGAA GAAAACTGTG TTTCATTTAA AGATGTTAAT 3051 TAAATGATTG AAACTTGAAA AAAAAAA BLAST Results No BLAST result Medline entries No Medlme entry Peptide information for frame 1 ORF from 49 bp to 981 bp; peptide length: 311 Category: putative protein Classification: unset 1 MADFPPPEEA FFSVASPEPA GPSGSPELVS SPAASSSSAT ALQIQPPGSP 51 DPPPAPPAPA PASSAPGHVA KLPQKEPVGC SKGGGPPRED VGAPLVTPSL 101 LQMVRLRSVG APGGAPTPAL GPSAPQKPLR RALSGRASPV PAPSSGLHAA 151 VRLKACSLAA SEGLSSAQPN GPPEAEPRPP QSPASTASFI FSKGSRKLQL 201 ERPVSPETQA DLQRNLVAEL RSISEQRPPQ APKKSPKAPP PVARKPSVGV 251 PPPASPSYPR AEPLTAPPTN GLPHTQDRTK RELAENGGVL QLVGPEEKMG 301 LPGSDSQKEL A BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphmcfl_lc23, frame 1 PIR:S49915 extensm-like protein - maize, N = 1, Score = 215, P = 6.1e-15 PIR:A28996 prolme-rich protein M14 precursor - mouse, N = 1, Score = 191, P = 3.8e-13 >PIR:S49915 extensin-like protein - maize Length = 1, 188 HSPs: Score = 215 (32.3 bits), Expect = 6.1e-15, P = 6.1e-15 Identities = 81/269 (30%), Positives = 115/269 (42%) Query: 5 PPPEEAFFS VASPEPAGPSGSPELVSSPAASSSSATALQIQPPGSP—DPPP A 55 PPP S V SP P P SP PA +SS ++ PP +P PPP + Sbjct: 598 PPPPAPVASPPPPVKSPPPPTPVASPP PPAPVASSPPPMKSPPPPTPVSSPPPPEKS 654 Query: 56 PPAPAPASSAPGHVAKLPQKEPVGCSKGGGPPREDVGAPLVTPSLLQMVRLRSVGAPGGA 115 PP P PA S P + P P K PP + + P + PS + P Sbjct: 655 PPPPPPAKSTPPP-EEYPT—PPTSVKSSPPPEKSLPPPTLIPSPPPQEKPTPPSTPSKP 711 Query: 116 PTPALGPSAPQKPLRRA-LSGRASPVPAPSSGLHAAVRLKACSLAASEGLSSAQPNGPPE 174 P+ PS P++P+ + ++SP PAP S +LA S + + PP Sbjct: 712 PSSPEKPSPPKEPVSSPPQTPKSSPPPAPVSSPPPTPVSSPPALAPVSSPPSVKSSPPPA 771 Query: 175 AEPRPPQSPAΞTASFIFSKGSRKLQLERPV-SPETQADLQRNLVAELRSISEQRPPQAPK 233 PP +P +S +Q+ P +P++ L V+ + + PP AP Sbjct: 772 PLSSPPPAPQVKΞS PPPVQVSSPPPAPKSSPPLAP—VSSPPQVEKTSPPPAPL 823 Query: 234 KSPKAPPPVARKPSVGV—PPPASPSYPRAEPLTAPPTNGLP 273 SP p + p v v ppp s p P+++PP P Sbjct: 824 SSPPLAPK-SSPPHVVVSSPPPVVKSSPPPAPVSSPPLTPKP 864 Score = 206 (30.9 bits), Expect = 9. le-14, P = 9. le-14 Identities = 82/261 (31%), Positives = 108/261 (41%) Query: 17 PEPAG-PSGSPELVΞΞPAASS SSATALQIQPPGSPDPPPAP PAPAPASSAPGHV 69 P P G P SP + PAAS+ S T + P P+P P P P P P +P Sbjct: 410 PTPGGGPPSSP-VPGKPAASAPMPSPHTPPDVSPEPLPEPSPVPAPAPMPMPTPHSPPAD 468 Query: 70 AKLPQKEPV-GCSKGGGPPREDVGAPLVTPSLLQMVRLRSVGAPGGAPTPALGPSAPQKP 128 +P PV G S P V P + +V+L AP G+P P + ++P P Sbjct: 469 DYVPPTPPVPGKΞPPATSPSPQVQPPAASTPPPSLVKLSPPQAPVGSPPPPVKTTSPPAP 528 Query: 129 LRRALSGRASPVPAPSSGLHAAVRLKACSLAASEGLSSAQPNGPPEAEPRPPQSPASTAS 188 + G SP P P S + +K+ A G + P PPE P PP AS Sbjct: 529 I GSPSP-PPPVSVVSPPPPVKSPPPPAPVG SPP—PPEKSPPPPAPVASPPP 577 Query: 189 FIFSKGSRKLQLERPVSPETQADLQRNLVAELRSISEQRPPQAPKKSPKAPPPVARKPS- 247 + S L P P ++ VA + PP P SP P PVA P Sbjct: 578 PVKSPPPPTLVASPP—PPVKSPPPPAPVASPPPPVKSPPPPTPVASPPPPAPVASSPPP 635 Query: 248 VGVPPP ASPSYPRAEPLTAPPTNGLPHTQD 277 + PPP +SP P P PP P ++ Sbjct: 636 MKSPPPPTPVSSPPPPEKSPPPPPPAKSTPPPEE 669 Score = 202 (30.3 bits), Expect = 2.9e-13, P = 2.9e-13 Identities = 81/254 (31%), Positives = 110/254 (43%) Query: 16 SPEPAGPSGSPELV—SSP—AASSSSATALQIQPPGSP-DPPPAPPAPAPASSAPGHVA 70 SP PA P SP L SSP SS ++ PP +P PP P PA S P HV+ Sbjct: 817 SPPPA-PLSSPPLAPKSSPPHVVVSSPPPVVKSSPPPAPVSSPPLTPKPA SPPAHVS 872 Query: 71 KLPQ KEPVGCSKGGGPPREDVGAPLVTPSLLQMVRLRSVGAPGGAPTPALGPSAPQ 126 P+ P + PP E +P TP L ++S P +P + P + Sbjct: 873 SPPEVVKPSTPPAPTTVISPPSEPKSSPPPTPVSLPPPIVKSSPPPAMVSΞPPMTPKSSP 932 Query: 127 KPLRRAL SGRASPVPAPSSGLHAAVRLKACSLAASEGLSSAQPNGPPEAEPRPPQSP 183 P+ + + ++SP PAP S A K+ A L P PPE + PP +P Sbjct: 933 PPVVVSSPPPTVKSSPPPAPVSSPPATP—KSSPPPAPVNL P—PPEVKSSPPPTP 984 Query: 184 ASTASFIFSKGSRKLQLERPVSPETQADLQRNLVAELRSISEQRPPQAPKKSPKAPPPVA 243 S+ + P PE ++ V+ + PP AP SP PPPV Sbjct: 985 VSSPPPAPKSSPPPAPMSΞPPPPEVKSPPPPAPVSSPPPPVKSPPPPAPVSSP—PPPVK 1042 Query: 244 RKPS VGVPPPASPΞYPRAEPLTAPP 268 P V PPP S P P+++PP Sbjct: 1043 SPPPPAPVSSPPPPVKSPPPPAPISSPP 1070 Score = 190 (28.5 bits), Expect = 7.9e-12, P = 7.9e-12 Identities = 74/264 (28%), Positives = 111/264 (42%) Query: 5 PPPEEAFFSVASPEPAGPSGSPELVSSPAAS-SSSATALQIQPPGSPDPPPAPPAPAPAS 63 PPP S PE + P P + P + T+++ PP PP P+P Sbjct: 639 PPPPTPVSSPPPPEKSPPPPPPAKSTPPPEEYPTPPTSVKSSPPPEKSLPPPTLIPSPPP 698 Query: 64 SAPGHVAKLPQKEPVGCSKGGGPPREDVGAPLVTPSLLQMVRLRSVGAPGGAPTPALGPS 123 P K P K PP+E V +P TP V +P PTP P Sbjct: 699 QEKPTPPSTPSKPPSSPEKPS-PPKEPVSSPPQTPK—SSPPPAPVSSP—PPTPVSSPP 753 Query: 124 APQKPLRRALSGRASPVPAPSSGLHAAVRLKACSLAASEGLSSAQPNGPPEAEPRPPQSP 183 A P+ S ++SP PAP S A ++K+ + + + P PP + PP +P Sbjct: 754 A-LAPVSSPPSVKSSPPPAPLSSPPPAPQVKS SPPPVQVSSP—PPAPKΞSPPLAP 806 Query: 184 ASTASFIFSKGSRKLQLERP-VSPETQADLQRNLVAELRSISEQRPPQAPKKSPKAPPPV 242 S+ + L P ++P++ +V+ + + PP AP SP P Sbjct: 807 VSSPPQVEKTSPPPAPLSSPPLAPKSSPP—HVVVSSPPPVVKSSPPPAPVSSPPLTPKP 864 Query: 243 ARKPS-VGVPP PASPSYPR AEPLTAPP 268 A P+ V PP P++P P +EP ++PP Sbjct: 865 ASPPAHVSSPPEVVKPSTPPAPTTVISPPSEPKSSPP 901 Score = 189 (28.4 bits). Expect = l.Oe-ll, P = l.Oe-11 Identities = 86/271 (31%), Positives = 112/271 (41%) Query: 5 PPPEEAFFSVASPEPAGPSGSPEL-VSSP—AASSSSATALQIQPPG—SPDPPPAP 56 PPP A S P P S P + VSSP A SS A PP PPPAP Sbjct: 768 PPP--APLSSPPPAPQVKSSPPPVQVSSPPPAPKSSPPLAPVSSPPQVEKTSPPPAPLSS 825 Query: 57 PAPAPASSAPGHVAKLPQKEPVGCSKGGGPPREDVGAPLVTPSLLQMVRLRSVGAPGGAP 116 P AP SS P V P PV S PP V +P +TP V +P Sbjct: 826 PPLAPKSSPPHVVVSSPP—PVVKSS PPPAPVSSPPLTPKPASPPA—HVSSPPEVV 878 Query: 117 TPALGPSAPQKPLRRALSGRASPVPAPSSGLHAAVRLKAC-SLAASEGL SSAQP 169 P+ P AP + ++SP P P S V+ ++ +S + SS P Sb j ct: 879 KPST-PPAPTTVISPPSEPKSSPPPTPVSLPPPIVKSSPPPAMVSSPPMTPKSSPPPWV 937 Query: 170 -NGPPEAEPRPPQSPASTASFIFSKGSRKLQLERPVSPETQADLQRNLVAELRSISEQRP 228 + PP + PP +P S+ + P PE ++ V+ + P Sb j ct: 938 SSPPPTVKSSPPPAPVSSPPATPKSSPPPAPVNLP-PPEVKSSPPPTPVSSPPPAPKSSP 996 Query: 229 PQAPKKΞPKAPPPVARKPS VGVPPPASPSYPRAEPLTAPP 268 P AP SP PPP + P V PPP S P P+++PP Sbjct: 997 PPAPMSSP—PPPEVKSPPPPAPVSΞPPPPVKSPPPPAPVSSPP 1038 Score = 181 (27.2 bits), Expect = 8.8e-ll, P = 8.8e-ll Identities = 73/277 (26%), Positives = 105/277 (37%) Query: 3 DFPPPEEAFFSVASPEPAGPSGSPELVSSPAASSSSATALQIQPP GSPDPP PA 55 D+ PP V P S SP+ V PAAΞ+ + +++ PP GSP PP + Sbjct: 469 DYVPPTPP VPGKSPPATSPSPQ-VQPPAASTPPPSLVKLSPPQAPVGSPPPPVKTTS 524 Query: 56 PPAPAPASSAPGHVAKL PQKEPVGCSKGGGPPREDVGAPLVTPSLLQMVRLRSVGA 111 PPAP + S P V+ + P K P + G PP + P P ++S Sbjct: 525 PPAPIGSPSPPPPVSVVΞPPPPVKSPPPPAPVGSPPPPEKSPPPPAPVASPPPPVKSPPP 584 Query: 112 PG—GAPTPALGPSAPQKPLRRA LSGRASPVPAPSSGLHAAVRLKACSLAASEGLSS 166 P +P P + P P+ + P P S A V + + + Sbjct: 585 PTLVASPPPPVKSPPPPAPVASPPPPVKSPPPPTPVASPPPPAPVASSPPPMKSPPPPTP 644 Query: 167 AQPNGPPEAEPRPPQSPASTASFIFSKGSRKLQLERPVSPETQADLQRNLVAELRSISEQ 226 PPE P PP PA + + ++ PE L+ + Sbjct: 645 VSSPPPPEKSP-PPPPPAKSTPPPEEYPTPPTSVKSSPPPEKSLP-PPTLIPSPPPQEKP 702 Query: 227 RPPQAPKKSPKAPP-PVARKPSVGVPPPASPSYPRAEPLTAPP 268 PP P K P +P P K V PP S P P+++PP Sbjct: 703 TPPSTPSKPPSSPEKPSPPKEPVSSPPQTPKSΞPPPAPVSSPP 745 Score = 177 (26.6 bits), Expect = 2.6e-10, P = 2.6e-10 Identities = 78/ 2 64 (29%), Positives = 105/264 (39%) Query: 5 PPPEEAFFSVASPEPAGP SGSPELVSSPAASSSSATALQIQPPGSP—DPPPAP— 56 PPP +P+PA P S PE+V P+ + T I PP P PPP P Sbjct: 850 PPPAPVSSPPLTPKPASPPAHVSSPPEVVK-PSTPPAPTTV—ISPPSEPKSSPPPTPVS 906 Query: 57 -PAPAPASSAPGHVAKLPQKEPVGCSKGGGPPREDVGAPLVTPSLLQMVRLRSVGAPGGA 115 P P SS P + P P PP V +P P++ V +P Sbjct: 907 LPPPIVKSSPPPAMVSSPPMTPKS SPPPVVVSSP—PPTVKSSPPPAPVSSPPAT 959 Query: 116 PTPALGPSAPQKPLRRALSGRASPVPAPSSGLHAAVRLKACSLAASEGLSSAQPNGPPEA 175 P + P+ P ++SP P P S A + S +SS P PPE Sbjct: 960 PKSSPPPAPVNLPPPEV KSSPPPTPVSSPPPAPK SSPPPAPMSSP-P—PPEV 1009 Query: 176 EPRPPQSPASTASFIFSKGSRKLQLERPVSPETQADLQRNLVAELRSISEQRPPQAPKKS 235 + PP +P S+ + P P ++ V+ + PP AP S Sbjct: 1010 KSPPPPAPVSSPPPPVKSPPPPAPVSSP-PPPVKSPPPPAPVSSPPPPVKSPPPPAPISS 1068 Query: 236 PKAPPPVARKPS VGVPPPASPSYPRAEPLTAPP 268 P PPPV P V PPP S P P+++PP Sbjct: 1069 P—PPPVKSPPPPAPVSSPPPPVKSPPPPAPVSSPP 1102 Score = 177 (26.6 bits), Expect = 2.6e-10, P = 2.6e-10 Identities = 82/267 (30%), Positives = 110/267 (41%) Query: 17 PEPAG-PSGSPELVSSPAASS SSATALQIQPPGSPDPPPAP PAPAPASSAPGHV 69 P P G P SP + PAAS+ S T + P P+P P P P P P +P Sbjct: 410 PTPGGGPPSSP-VPGKPAASAPMPSPHTPPDVSPEPLPEPSPVPAPAPMPMPTPHSPPAD 468 Query: 70 AKLPQKEPV-GCSKGGGPPREDVGAPLVTPSLLQMVRLRSVGAPGGAPTPALGPSAPQKP 128 +P PV G S P V P + +V+L AP G+P P + ++P P Sbjct: 469 DYVPPTPPVPGKSPPATSPSPQVQPPAASTPPPSLVKLSPPQAPVGSPPPPVKTTSPPAP 528 Query: 129 LRRALSGRASPVPAPSSGLHAAVRLKACSLAASEGLSSAQPNGPPEAEPRPPQSPASTAS 188 + G SP P P S + +K+ A G + P PPE P PP AS Sbjct: 529 I GSPSP-PPPVSVVSPPPPVKSPPPPAPVG SPP—PPEKSPPPPAPVASPPP 577 Query: 189 FIFSKGSRKLQLERPV SPETQADLQRNLVAELRS ISEQRPPQA PK 233 + S L P SP A + + ++S ++ PP P Sbjct: 578 PVKSPPPPTLVASPPPPVKSPPPPAPVA-SPPPPVKSPPPPTPVASPPPPAPVASSPPPM 636 Query: 234 KSPKAPPPVARKP SVGVPPPASPSYPRAEPLTAPPTN 270 KSP P PV+ P PPP + S P E PPT+ Sbjct: 637 KSPPPPTPVSSPPPPEKSPPPPPPAKSTPPPEEYPTPPTS 676 Score = 170 (25.5 bits), Expect = 1.6e-09, P = 1.6e-09 Identities = 78/279 (27%), Positives = 108/279 (38%) Query: 5 PPPEEAFFSVASPEPAGPSGSPELVSSPAASSSSATALQIQPPGSPDPPPAPPAPAPASS 64 PP S S + P +P + P SS A+ PP +P +PP P SS Sbjct: 883 PPAPTTVISPPSEPKSSPPPTPVSLPPPIVKSSPPPAMVSSPPMTPKS—SPP-PVVVSS 939 Query: 65 APGHVAKLPQKEPVGCSKGGGPPREDVGAPLVTPSLLQMVRLRSVGAPG—GAPTPALGP 122 P V P PV PP +P P L ++S P +P PA Sbjct: 940 PPPTVKSSPPPAPVS SPPATPKSSPPPAPVNLPPPEVKSSPPPTPVSSPPPAPKS 994 Query: 123 SAPQKPLRRALSG--RASPVPAPSSGLHAAVRLKACSLAASEGLSSAQPNGPPEAEPRPP 180 S P P+ ++ P PAP S V+ S +SS P PP + PP Sbjct: 995 SPPPAPMSSPPPPEVKSPPPPAPVSSPPPPVK SPPPPAPVSS--P--PPPVKSPPP 1046 Query: 181 QSPASTASFIFSKGSRKLQLERPVSPETQADLQRNLVAELRSISEQRPPQAPKKSPKAPP 240 +P S+ + P P ++ V+ + PP AP SP PP Sbjct: 1047 PAPVΞSPPPPVKSPPPPAPISSP-PPPVKSPPPPAPVSSPPPPVKSPPPPAPVSSP—PP 1103 Query: 241 PVARKPS VGVPPPAS PSYPRAEPLTAPPTNGLPHTQDRTKREL 283 P+ P V PPPA PS P P+++PP P + ++ L Sbjct: 1104 PIKSPPPPAPVΞSPPPAPVKPPSLPPPAPVSSPPPVVTPAPPKKEEQSL 1152 Score = 169 (25.4 bits). Expect = 2. le-09, P = 2. le-09 Identities = 75/266 (28%), Positives = 104/266 (39%) Query: 3 DFPPPEEAFFSVASPEPAGPSGSPELVSSPAASSSSATALQIQPP GSPDPP PA 55 D+ PP V P S SP+ V PAAS+ + +++ PP GSP PP + Sbjct: 469 DYVPPTPP VPGKSPPATSPSPQ-VQPPAASTPPPSLVKLSPPQAPVGSPPPPVKTTS 524 Query: 56 PPAPAPASSAPGHVAKLPQKEPVGCSKGGGPPREDVGAPLVTPSLLQMVRLRSVGAPGGA 115 PPAP + S P V+ + PV PP VG+P P V +P Sbjct: 525 PPAPIGSPSPPPPVSVVSPPPPVKSP PPPAPVGSP—PPPEKSPPPPAPVASP 575 Query: 116 PTPALGPSAPQKPLRRALSGRASPVPAPSSGLHAAVRLKACSLAASEGLSSAQPNGPPEA 175 P P P P ++ P PAP + V+ S ++S P P + Sbjct: 576 PPPVKSPPPPTLVASPPPPVKSPPPPAPVASPPPPVK SPPPPTPVASPPPPAPVAS 631 Query: 176 EPRPPQSPASTASFIFSKGSRKLQLERPVSPETQADLQRNLVAELRSISEQRPPQAPKKS 235 P P +SP K P P S+ PP+ Sbjct: 632 SPPPMKSPPPPTPVSSPPPPEKSP—PPPPPAKSTPPPEEYPTPPTSVKSSPPPEKSLPP 689 Query: 236 PK APPPVARK—PSVGVPPPASPSYPRA—EPLTAPP 268 P +PPP + PS PP+SP P EP+++PP Sbjct: 690 PTLIPSPPPQEKPTPPSTPSKPPΞSPEKPSPPKEPVSSPP 729 Score = 168 (25.2 bits), Expect = 2.7e-09, P = 2.7e-09 Identities = 75/267 (28%), Positives = 102/267 (38%) Query: 2 ADFPPPEEAFFSVASPE-PAGPSGSPELVSSPAASSSSATALQIQPPGSPDPP-PAPPAP 59 A PPP + ++ P+ P G P +SP A S + SP PP +PP P Sbjct: 496 ASTPPP—SLVKLΞPPQAPVGSPPPPVKTTSPPAPIGSPSPPPPVΞVVSPPPPVKSPPPP 553 Query: 60 APASSAPGHVAKLPQKEPVGCSKGGGPPREDVGAPLVTPSLLQMVRLRSVGAPGGAPTPA 119 AP S P P PV PP + P + S V+ AP +P P Sbjct: 554 APVGSPPPPEKSPPPPAPVASPP PPVKΞPPPPTLVASPPPPVKSPPPPAPVASPPPP 610 Query: 120 LGPSAPQKPLRRALSGRASPVPAPSSGLHAAVRLKACSL-AASEGLSSAQPNGPPEAEPR 178 + P P+ + P PAP + ++ +S P PP A+ Sbjct: 611 VKSPPPPTPVA SPPPPAPVASSPPPMKSPPPPTPVSSPPPPEKSPPPPPPAKST 664 Query: 179 PP--QSPASTASFIFSKGSRKLQLERPV SPETQADLQRNLVAELRSISEQRPPQAPK 233 PP + P S S K L P SP Q S ++P +P Sbjct: 665 PPPEEYPTPPTSVKSSPPPEK-SLPPPTLIPSPPPQEKPTPPSTPSKPPSSPEKP—SPP 721 Query: 234 KSPKAPPPVARKPSVGVPPPASPSYPRAEPLTAPP 268 K P + PP K S PPPA S P P+++PP Sbjct: 722 KEPVSSPPQTPKSS PPPAPVSSPPPTPVSSPP 753 Score = 166 (24.9 bits), Expect = 4.6e-09, P = 4.6e-09 Identities = 81/268 (30%), Positives = 108/268 (40%) Query: 5 PPPEEAF FSVASPEPAGPSGSPE-LVSSPAAΞSSS ATALQIQPPGSPDPPP-- 54 PPPE++ VASP P Ξ P LV+SP S A PP PPP Sbjct: 560 PPPEKSPPPPAPVASPPPPVKSPPPPTLVASPPPPVKSPPPPAPVASPPPPVKSPPPPTP 619 Query: 55 --APPAPAPASSAPGHVAKLPQKEPVGC SKGGGPPREDVGAPLVTPSLLQMVRLRS 108 +PP PAP +S+P + P PV K PP P ++S Sbjct: 620 VASPPPPAPVASSPPPMKSPPPPTPVSΞPPPPEKSPPPPPPAKSTPPPEEYPTPPTSVKS 679 Query: 109 VGAPGGA-PTPALGPSAPQKPLRRALSGRASPVPAPSSGLHAAVRLKACSLAASEGLSSA 167 P + P P L PS P P + + ++P PSS + + Ξ SS Sbjct: 680 SPPPEKSLPPPTLIPSPP—PQEKP-TPPSTPSKPPSSPEKPSPPKEPVSSPPQTPKSSP 736 Query: 168 QPNGPPEAEPRPPQSPASTASFIFSKGSRKLQLERPVSPETQADLQRNLVAELRSISEQR 227 P P P SP + A + S Ξ K P +P + + + + Sbjct: 737 PPAPVSSPPPTPVΞSPPALAP-VSSPPSVKSS--PPPAPLSSPPPAPQVKSSPPPVQVSS 793 Query: 228 PPQAPKKSPKAPPPVARKPSVGVPPPASPSYPRAEPLTAPP 268 PP APK SP P+A P V PP + P PL++PP Sbjct: 794 PPPAPKSSP PLA--P-VSΞPPQVEKTSPPPAPLSSPP 827 Score = 165 (24.8 bits), Expect = 6.0e-09, P = 6.0e-09 Identities = 79/264 (29%), Positives = 105/264 (39%) Query: 5 PPPEEAFFSVASPEPAG-PSGSP--ELVSSPAASSSSATALQIQPPGSPDPPP-APPAPA 60 PPP + + + P P G PS P +VS P S P GSP PP +PP PA Sbjct: 517 PPPVK TTSPPAPIGSPSPPPPVSVVSPPPPVKSPPPPA PVGSPPPPEKSPPPPA 570 Query: 61 PASSAPGHVAKLPQKEPVGCSKG GGPPREDVGAP LVTPSLLQMVRLRSVGAPGG 114 P +S P V P V PP V +P + +P V AP Sbjct: 571 PVASPPPPVKSPPPPTLVASPPPPVKSPPPPAPVAΞPPPPVKSPPPPTPVASPPPPAPVA 630 Query: 115 APTPALGPSAPQKPLRRALSGRASPVPAP SSGLHAAVRLKACSLAASEGLSSAQPNG 171 + P + P P+ SP P P S+ S+ +S + P Sbjct: 631 ΞSPPPMKSPPPPTPVSSPPPPEKSPPPPPPAKSTPPPEEYPTPPTSVKSSPPPEKSLP— 688 Query: 172 PPEAEPRPPQSPASTASFIFSKGSRKLQLERPVSPETQADLQRNLVAELRSISEQRPPQA 231 PP P PP T SK P SPE + + V+ + PP A Sbjct: 689 PPTLIPSPPPQEKPTPPΞTPSKP PSSPEKPSP-PKEPVSSPPQTPKSSPPPA 739 Query: 232 PKKSPKAPPPVARKPSVGV—PPPASPSYPRAEPLTAPP 268 P SP P PV+ P++ PP+ S P PL++PP Sbjct: 740 PVSSPP-PTPVSSPPALAPVSSPPSVKSSPPPAPLSSPP 777 Score = 162 (24.3 bits), Expect = 1.3e-08, P = 1.3e-08 Identities = 76/27 2 ( 2 7%), Positives = 99/ 2 7 2 (36%) Query: 2 ADFPPPEEAFFSVASPEPAG-PSGSPELVSSPAASSSSATALQIQPPGSPDPPPAPPAPA 60 A P P SPEP PS P P + S A PP P P +PPA + Sbjct: 427 ASAPMPSPHTPPDVSPEPLPEPSPVPAPAPMPMPTPHSPPADDYVPPTPPVPGKSPPATS 486 Query: 61 PASSAPGHVAKLPQKEPVGCSKGGGPPREDVGAPLVTPSLLQMVRLRSVGAPGGAPTP-- 118 P+ A P V S PP+ VG+P P V+ S AP G+P+P Sbjct: 487 PSPQVQPPAASTPPPSLVKLS PPQAPVGSP—PPP VKTTSPPAPIGSPSPPP 536 Query: 119 ALGPSAPQK-PLRRALSGRASPVPAPSSGLHAAVRLKACSLAASEGLSSAQPNGPPE 174 + P P K P A G SP P S A S + + PP Sbjct: 537 PVSVVSPPPPVKSPPPPAPVG—SPPPPEKSPPPPAPVASPPPPVKΞPPPPTLVASPPPP 594 Query: 175 AEPRPPQSPAΞTASFIFSKGSRKLQLERPVSPETQADLQRNLVAELRSISEQRPPQAPKK 234 + PP +P ++ + P P A + + PP P+K Sbjct: 595 VKSPPPPAPVASPPPPVKSPPPPTPVASPPPPAPVASSPPPMKSPPPPTPVSSPPP-PEK 653 Query: 235 SPKAPPPVARKPSVGVPPPASPSYPRAEPLTAPPTNGLP 273 SP PPP P PP P+ P + + PP LP Sbjct: 654 SPPPPPPAKSTP PPEEYPTPPTSVKSSPPPEKSLP 688 Score = 159 (23.9 bits), Expect = 2.8e-08, P = 2.8e-08 Identities = 77/264 (29%), Positives = 103/264 (39%) Query: 5 PPPEEAFFSVASPEPAGPSGSPELVSSPAASSSSATALQIQPPGSP—DPPPAP PAP 59 PPP V+SP P P SP P SS ++ PP +P PP P P P Sbjct: 916 PPPA MVSSP-PMTPKSSPP PVVVSSPPPTVKSSPPPAPVSSPPATPKSΞPPP 966 Query: 60 APASSAPGHVAKLPQKEPVGCSKGGGPPREDVGAPLVTPSLLQMVRLRSVGAPGGAPTPA 119 AP + P V P PV S P AP+ +P + V+ AP +P P Sbjct: 967 APVNLPPPEVKSSPPPTPVS-SPPPAPKSSPPPAPMSSPPPPE-VKSPPPPAPVSSPPPP 1024 Query: 120 LGPSAPQKPLRRALΞG-RASPVPAPSSGLHAAVRLKACSLAASEG LSSAQPNGPPEA 175 + P P+ ++ P PAP S V+ S + S P P + Sbjct: 1025 VKSPPPPAPVSSPPPPVKSPPPPAPVSSPPPPVKSPPPPAPIΞSPPPPVKSPPPPAPVSS 1084 Query: 176 EPRPPQSPASTASFIFSKGSRKLQLERPVSPETQADLQRNLVAELRSISEQRPPQAPKKS 235 P P +SP A S ++ P P A + A ++ S PP AP S Sbjct: 1085 PPPPVKSPPPPAPV SSPPPPIKSPPPP APVSSPPPAPVKPPS--LPPPAPVSS 1135 Query: 236 PK--APPPVARKPSVGVPPPA-SPSYPRAEPLTAPP 268 P P +K +PPPA S P + PP Sbjct: 1136 PPPVVTPAPPKKEEQSLPPPAESQPPPSFNDIILPP 1171 Score = 143 (21.5 bits), Expect = 1.8e-06, P = 1.8e-06 Identities = 59/179 (32%), Positives = 77/179 (43%) Query: 3 DFPPPEEAFFSVASPEP-AGPSGSPELVSSPAASSSSATA-LQIQPPGSP—DPPP A 55 + PPPE Ξ P P + P +P+ PA SS ++ PP +P PPP + Sbjct: 970 NLPPPEVK—SSPPPTPVSSPPPAPKSSPPPAPMSSPPPPEVKSPPPPAPVSSPPPPVKS 1027 Query: 56 PPAPAPASSAPGHVAKLPQKEPVGCΞKGGGPPREDVGAPLVTPSLLQMVRLRSVGAPGGA 115 PP PAP SS P V P PV PP + P Ξ V+ AP + Sbjct: 1028 PPPPAPVSSPPPPVKSPPPPAPVSSPP PPVKSPPPPAPISSPPPPVKSPPPPAPVSS 1084 Query: 116 PTPALGPSAPQKPLRRALSG-RASPVPAPSSGLHAAVRLKACSLAASEGLSSAQPNGPPE 174 P P + P P+ ++ P PAP S A +K SL +SS P PP Sbjct: 1085 PPPPVKSPPPPAPVSSPPPPIKSPPPPAPVSSPPPAP-VKPPSLPPPAPVSS—P—PPV 1139 Query: 175 AEPRPPQ 181 P PP+ Sbjct: 1140 VTPAPPK 1146 Score = 133 (20.0 bits), Expect = 2.3e-05, P = 2.3e-05 Identities = 50/132 (37%), Positives = 59/132 (44%) Query: 1 MADFPPPEEAFFSVASPEPAGP-SGSPELVSSP AASSSSATALQIQPPGSP—DPPP 54 M+ PPPE V SP P P S P V SP A SS ++ PP +P PPP Sbjct: 1001 MSSPPPPE VKSPPPPAPVSSPPPPVKSPPPPAPVSSPPPPVKSPPPPAPVSSPPP 1055 Query: 55 APPAPAPASSAPGHVAKLPQKEPVGCSKG GGPPREDVGAPLVTPSLLQMVRLRS 108 +PP PAP SS P V P PV PP V +P P + Sbjct: 1056 PVKSPPPPAPISSPPPPVKSPPPPAPVSSPPPPVKSPPPPAPVSSP—PPPIKSPPPPAP 1113 Query: 109 VGAPGGAPT—PALGPSAP 125 V +P AP P+L P AP Sbjct: 1114 VSSPPPAPVKPPSLPPPAP 1132 Score = 110 (16.5 bits), Expect = 8.0e-03, P = 8.0e-03 Identities = 41/121 (33%), Positives = 49/121 (40%) Query: 5 PPPEEAFFS VASPEPAGP-SGSPELVSSP AASSSSATALQIQPPGSP—DPPP 54 PPP S V SP P P S P V SP A SS ++ PP +P PPP Sbjct: 1060 PPPPAPISSPPPPVKSPPPPAPVSSPPPPVKΞPPPPAPVSSPPPPIKSPPPPAPVSSPPP 1119 Query: 55 AP PAPAPASSAPGHVAKLPQKEPVGCSKGGGPPREDVGAPLVTPSLLQMVRLRS 108 AP P PAP SS P V P K+ + PP E P +L + Sbjct: 1120 APVKPPSLPPPAPVSSPPPVVTPAPPKKE EQSLPPPAESQPPPSFNDIILPPIMANK 1176 Query: 109 VGAP 112 +P Sbjct: 1177 YASP 1180 Score = 108 (16.2 bits), Expect = 1.3e-02, P = 1.3e-02 Identities = 46/155 (29%), Positives = 67/155 (43%) Query: 114 GAPTPALGPSAPQKPLRRALSGRASPVPAPSSGLHAAVR-LKACΞ-LAASEGLSSAQPNG 171 G PTP GP + P + A S +P+P+P + + L S + A + P+ Sbjct: 408 GYPTPGGGPPSSPVPGKPAAS APMPSPHTPPDVSPEPLPEPΞPVPAPAPMPMPTPHS 464 Query: 172 PPEAEPRPPQSPASTASFIFSKGSRKLQLERPVSPETQ ADLQRNLVAELRSISEQR 227 PP + PP P S + S ++Q +P + Q + + + Sbjct: 465 PPADDYVPPTPPVPGKSPPATSPSPQVQPPAASTPPPSLVKLSPPQAPVGSPPPPVKTTS 524 Query: 228 PPQAPKKSPKAPPPVARKPSVGVPPPASPSYPRAEPLTAPP 268 PP AP SP PPPV SV PPP S P P+ +PP Sbjct: 525 PP-APIGSPSPPPPV SVVSPPPPVKSPPPPAPVGSPP 560 Pedant information for DKFZphmcfl_lc23, frame 1 Report for DKFZphmcfl_lc23.1 [LENGTH] 311 [MW] 31534.58 [pi] 9.48 [KW] All Alpha [KW] LOW COMPLEXITY 38.59 % SEQ MADFPPPEEAFFSVASPEPAGPSGSPELVSSPAASSSSATALQIQPPGSPDPPPAPPAPA SEG xxxxxxxxxxxxxxx..xxxxxxxxxxxx....xxxxxxxxxxxxxxx PRD ccccccccccccccccccccccccccccccccccccccceeeeecccccccccccccccc SEQ PASSAPGHVAKLPQKEPVGCSKGGGPPREDVGAPLVTPSLLQMVRLRSVGAPGGAPTPAL SEG xxxxxx xxxxxxxxxxx PRD cccccccccccccccccccccccccccccccccccchhhhhhhhhhhccccccccccccc SEQ GPSAPQKPLRRALSGRASPVPAPSSGLHAAVRLKACSLAASEGLSSAQPNGPPEAEPRPP SEG xxxxx xxxxxxxxxxxxx PRD cccccchhhhhhhhhcccccccccchhhhhhhhhhhhhhhhccccccccccccccccccc SEQ QSPASTASFIFSKGSRKLQLERPVSPETQADLQRNLVAELRΞISEQRPPQAPKKSPKAPP SEG xxxxx xxxxxxxxxxxxxxx PRD ccccccceeeecccchhhhhccccccchhhhhhhhhhhhhhhhccccccccccccccccc SEQ PVARKPSVGVPPPASPSYPRAEPLTAPPTNGLPHTQDRTKRELAENGGVLQLVGPEEKMG SEG xxxxxxxxxxxxxxxxxxxxxxx PRD ccccccccccccccccccccccccccccccccccccchhhhhhhcccceeeccccccccc SEQ LPGSDΞQKELA SEG PRD ccccccccccc (No Prosite data available for DKFZphmcfl_lc23.1) (No Pfam data available for DKFZphmcfl_lc23.1) DKFZphmcfl_lel5 group: transmembrane protein DKFZphmcfl_lel5 encodes a novel 454 ammo acid protein with similarity to C. elegans proteins and transporter proteins . The novel protein is similar to the PTR2 family of proton/oligopeptide symporter proteins and the D-xylose-proton symporter. Thus, the protein is a transporter of a so far unknown compound . The new protein can find application as a new transporter in eukaryotic cells, e.g. in drug transport into cells. similarity to D-XYLOΞE TRANSPORTER membrane regions : 9 complete cDNA, complete eds, EST hits matchs cDNA encoding cell growth inhibiting factor (E12646) Sequenced by DKFZ Locus : unknown Insert length: 1957 bp Poly A stretch at pos. 1947, polyadenylation signal at pos. 1929 1 GGTGCAGCGC CCGGGCTGAG CGACAGCAAG TGCAGCGGGC TCCTACCCCG 51 GGTGAGGGGT GGCCTCCGCG TGGGATCGTG CCCTCTTCAG CCCGCTCCTG 101 TCCCCGACAT CACGTGTATT CCGCACGTCC CCTCCGCGCT GTGTGTCTAC 151 TGAGACGGGG AGGCGTGACA GGGCCCGGGT CCCTTCTCAG TGGTGCTCTG 201 TGCTTCAGGG CAAGCTCCCC GTCTCCGGGC GCACTTCCCT CGCCTGTGTT 251 CGGTCCATCC TCCTTTCTCC AGCCTCCTCC CCTCGCAGGT GGGATCGTCG 301 GTGGGACCGG AGCGCGGGCG GGCGCGGCCC CCCGGGACCA TGGCCGGGTC 351 CGACACCGCG CCCTTCCTCA GCCAGGCGGA TGACCCGGAC GACGGGCCAG 401 TGCCTGGCAC CCCGGGGTTG CCAGGGTCCA CGGGGAACCC GAAGTCCGAG 451 GAGCCCGAGG TCCCGGACCA GGAGGGGCTG CAGCGCATCA CCGGCCTGTC 501 TCCCGGCCGT TCGGCTCTCA TAGTGGCGGT GCTGTGCTAC ATCAATCTCC 551 TGAACTACAT GGACCGCTTC ACCGTGGCTG TGTTCATCTC CAGTTACATG 601 GTGTTGGCAC CTGTGTTTGG CTACCTGGGT GACAGGTACA ATCGGAAGTA 651 TCTCATGTGC GGGGGCATTG CCTTCTGGTC CCTGGTGACA CTGGGGTCAT 701 CCTTCATCCC CGGAGAGCAT TTCTGGCTGC TCCTCCTGAC CCGGGGCCTG 751 GTGGGGGTCG GGGAGGCCAG TTATTCCACC ATCGCGCCCA CTCTCATTGC 801 CGACCTCTTT GTGGCCGACC AGCGGAGCCG GATGCTCAGC ATCTTCTACT 851 TTGCCATTCC GGTGGGCAGT GGTCTGGGCT ACATTGCAGG CTCCAAAGTG 901 AAGGATATGG CTGGAGACTG GCACTGGGCT CTGAGGGTGA CACCGGGTCT 951 AGGAGTGGTG GCCGTTCTGC TGCTGTTCCT GGTAGTGCGG GAGCCGCCAA 1001 GGGGAGCCGT GGAGCGCCAC TCAGATTTGC CACCCCTGAA CCCCACCTCG 1051 TGGTGGGCAG ATCTGAGGGC TCTGGCAAGA AATCTCATCT TTGGACTCAT 1101 CACCTGCCTG ACCGGAGTCC TGGGTGTGGG CCTGGGTGTG GAGATCAGCC 1151 GCCGGCTCCG CCACTCCAAC CCCCGGGCTG ATCCCCTGGT CTGTGCCACT 1201 GGCCTCCTGG GCTCTGCACC CTTCCTCTTC CTGTCCCTTG CCTGCGCCCG 1251 TGGTAGCATC GTGGCCACTT ATATTTTCAT CTTCATTGGA GAGACCCTCC 1301 TGTCCATGAA CTGGGCCATC GTGGCCGACA TTCTGCTGTA CGTGGTGATC 1351 CCTACCCGAC GCTCCACCGC CGAGGCCTTC CAGATCGTGC TGTCCCACCT 1401 GCTGGGTGAT GCTGGGAGCC CCTACCTCAT TGGCCTGATC TCTGACCGCC 1451 TGCGCCGGAA CTGGCCCCCC TCCTTCTTGT CCGAGTTCCG GGCTCTGCAG 1501 TTCTCGCTCA TGCTCTGCGC GTTTGTTGGG GCACTGGGCG GCGCAGCCTT 1551 CCTGGGCACC GCCATCTTCA TTGAGGCCGA CCGCCGGCGG GCACAGCTGC 1601 ACGTGCAGGG CCTGCTGCAC GAAGCAGGGT CCACAGACGA CCGGATTGTG 1651 GTGCCCCAGC GGGGCCGCTC CACCCGCGTG CCCGTGGCCA GTGTGCTCAT 1701 CTGAGAGGCT GCCGCTCACC TACCTGCACA TCTGCCACAG CTGGCCCTGG 1751 GCCCACCCCA CGAAGGGCCT GGGCCTAACC CCTTGGCCTG GCCCAGCTTC 1801 CAGAGGGACC CTGGGCCGTG TGCCAGCTCC CAGACACTAC ATGGGTAGCT 1851 CAGGGGAGGA GGTGGGGGTC CAGGAGGGGG ATCCCTCTCC ACAGGGGCAG 1901 CCCCAAGGGC TCGGTGCTAT TTGTAACGGA ATAAAATTTG TAGCCAGAAA 1951 AAAAAAA BLAST Results Entry E12646 from database EMBL: cDNA encoding cell growth inhibiting factor. Score = 3046, P = 2.2e-131, identities = 640/659 Medline entries No Medline entry Peptide information for frame 1 ORF from 340 bp to 1701 bp; peptide length: 454 Category: similarity to known protein 1 MAGΞDTAPFL SQADDPDDGP VPGTPGLPGS TGNPKSEEPE VPDQEGLQRI 51 TGLSPGRSAL IVAVLCYINL LNYMDRFTVA VFISSYMVLA PVFGYLGDRY 101 NRKYLMCGGI AFWSLVTLGS SFIPGEHFWL LLLTRGLVGV GEASYSTIAP 151 TLIADLFVAD QRSRMLSIFY FAIPVGSGLG YIAGSKVKDM AGDWHWALRV 201 TPGLGVVAVL LLFLVVREPP RGAVERHSDL PPLNPTSWWA DLRALARNLI 251 FGLITCLTGV LGVGLGVEIS RRLRHSNPRA DPLVCATGLL GSAPFLFLSL 301 ACARGSIVAT YIFIFIGETL LSMNWAIVAD ILLYVVIPTR RSTAEAFQIV 351 LSHLLGDAGS PYLIGLISDR LRRNWPPSFL SEFRALQFSL MLCAFVGALG 401 GAAFLGTAIF IEADRRRAQL HVQGLLHEAG STDDRIVVPQ RGRSTRVPVA 451 SVLI BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphmcfl_lel5, frame 1 TREMBL :CEC13C4_1 gene: "C13C4.5"; Caenorhabditis elegans cosmid C13C4, N = 3, Score = 441, P = 5.2e-76 TREMBL :CEC39E9_10 gene: "C39E9.10"; Caenorhabditis elegans cosmid C39E9, N = 2, Score = 449, P = 8.2e-69 TREMBL :CEF09A5_1 gene: "F09A5.1"; Caenorhabditis elegans cosmid F09A5, N = 3, Score = 413, P = 9.1e-60 TREMBL:ATF6H11_18 gene: "F6H11.180"; product: "predicted protein"; Arabidopsis thaliana DNA chromosome 5, BAC clone F6H11 (ESSAII project), N = 3, Score = 193, P = 2.5e-24 SWISSPROT :XYLT_LACBR D-XYLOSE-PROTON SYMPORT (D-XYLOSE TRANSPORTER)., N = 1, Score = 180, P = 7.9e-ll >TREMBL:CEC39E9_10 gene: "C39E9.10"; Caenorhabditis elegans cosmid C39E9 Length = 488 HSPs: Score = 449 (67.4 bits), Expect = 8.2e-69, Sum P(2) = 8.2e-69 Identities = 88/204 (43%), Positives = 125/204 (61%) Query: 58 SALIVAVLCYINLLNYMDRFTVAVFISSYMVLAPVFGYLGDRYNRKYLMCGGIAFWSLVT 117 + ++ V Y N+ + + VF+ S+MV +PV GYLGDR+NRK++M G+ W Sbjct: 29 AGVLTQVQTYYNISDSLGGLIQTVFLISFMVFSPVCGYLGDRFNRKWIMIIGVGIWLGAV 88 Query: 118 LGSSFIPGEHFWLLLLTRGLVGVGEASYSTIAPTLIADLFVADQRSRMLSIFYFAIPVGS 177 LGSΞF+P HFWL L+ R VG+GEASYS +AP+LI+D+F +RS + IFYFAIPVGS Sbjct: 89 LGSSFVPANHFWLFLVLRSFVGIGEASYSNVAPSLISDMFNGQKRSTVFMIFYFAIPVGS 148 Query: 178 GLGYIAGSKVKDMAGDWHWALRVTPGLGVVAVLLLFLVVREPPRGAVER HSDLPPL 233 GLG+I GS V + G W W +RV+ G++ ++ L L EP RGA ++ D+ Sbjct: 149 GLGFIVGSNVATLTGHWQWGIRVSAIAGLIVMIALVLFTYEPERGAADKAMGESKDVVVT 208 Query: 234 NPTSWWADLRALARNLIFGLITCLTG 259 T++ DL L + L+ C G Sbjct: 209 TNTTYLEDLVILLKTPT—LVACTWG 232 Score = 267 (40.1 bits), Expect = 8.2e-69, Sum P(2) = 8.2e-69 Identities = 74/212 (34%), Positives = 113/212 (53%) Query: 249 LIFGLITCLTGVLGVGLGVEISRRL RHSNPRADPLVCATGLLGSAPFLFLSL 300 L FG IT G++GV G +S+ L R RA PLV G L +APFL + + Sbjct: 277 LYFGAITTAGGLIGVIFGSMLSKWLVAGWGPFRRLQTDRAQPLVAGGGALLAAPFLLIGM 336 Query: 301 ACARGSIVATYIFIFIGETLLSMNWAIVADILLYVVIPTRRSTAEAFQIVLSHLLGDAGS 360 S+V YI IF G T + NW + D+L V+ P RRSTA ++ +++SHL GDA Sbjct: 337 IFGDKSLVLLYIMIFFGITFMCFNWGLNIDMLTTVIHPNRRSTAFSYFVLVSHLFGDASG 396 Query: 361 PYLIGLISDRLRRN—WPPSFLΞEFRALQFSLMLCAFVGALGGAAFLGTAIFIEADRR— 416 PYLIGLISD +R +P ++ +L + C + L + +++ + +DR+ Sbjct: 397 PYLIGLIΞDAIRHGSTYPKD QYHSLVSATYCCVALLLLSAGLYFVSSLTLVΞDRKKF 453 Query: 417 RAQLHVQGLLHEA—GSTD—DRIVVPQRGRSTRV 447 RA++ + L + STD +RI + S+R+ Sbjct: 454 RAEMGLDDLQΞKPIRTSTDSLERIGINDDVASSRL 488 Score = 70 (10.5 bits), Expect = 5.9e-24, Sum P(2) = 5.9e-24 Identities = 25/89 (28%), Positives = 41/89 (46%) Query: 62 VAVLCYINLLNYMDRFTVAVFISSYMVLAPVFGYLGDRYNRKYLMCGGIAFWSLVT—LG 119 V L +NLLNY+DR+TVA ++ + LG +L+ +S V LG Sbjct: 11 VTALFVVNLLNYVDRYTVAGVLTQVQTYYNISDSLGGLIQTVFLI—SFMVFSPVCGYLG 68 Query: 120 SSFIPGEHFWLLLLTRGLVGVGEASYSTIAP 150 F W++++ G + +G S+ P Sbjct: 69 DRF NRKWIMIIGVG-IWLGAVLGSSFVP 95 Pedant information for DKFZphmcfl_lel5, frame 1 Report for DKFZphmcfl_lel5.1 [LENGTH] 454 [MW] 49013.35 [pi] 7.66 [HOMOL] TREMBL :CEC13C4_1 gene: "C13C4.5"; Caenorhabditis elegans cosmid C13C4 2e-51 [BLOCKS] BL01022D [PROSITE] MYRISTYL 11 [PROSITE] CAMP_PHOSPHO_SITE 1 [PROSITE] CK2_PHOSPHO_SITE 3 [PROSITE] PROKAR_LIPOPROTEIN 1 [PROSITE] GLYCOSAMINOGLYCAN 1 [PROSITE] PKC_PHOSPHO_SITE 4 [KW] TRANSMEMBRANE 8 [KW] LOW_COMPLEXITY 15.42 % SEQ MAGSDTAPFLSQADDPDDGPVPGTPGLPGSTGNPKSEEPEVPDQEGLQRITGLSPGRSAL SEG xxxxxxxxxxxxxxxx PRD cccccceeeeeecccccccccccccccccccccccccccccccccceeeecccccchhhh MEM MMMMMMMMMMMMMMMMMMMMMMM SEQ IVAVLCYINLLNYMDRFTVAVFISSYMVLAPVFGYLGDRYNRKYLMCGGIAFWSLVTLGS SEG PRD hhhhhhhhccccccccceeeeeehhhhheeeecccccccccceeeeeeeccceeeeeecc MEM MMMMMM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM SEQ SFIPGEHFWLLLLTRGLVGVGEASYSTIAPTLIADLFVADQRΞRMLSIFYFAIPVGSGLG SEG xxxxxxxxxxxx PRD cccccchhhhhhhhhhccccccceeeeecceeeccccccccchhhhheeeeeecccccce MEM MMMMMMMMMMMMMMMM MMMMMMMMMMMMMMMM SEQ YIAGΞKVKDMAGDWHWALRVTPGLGVVAVLLLFLVVREPPRGAVERHSDLPPLNPTSWWA SEG xxxxxxxxxxxxx PRD eeecccccccccccceeeeeeccchhhhhhhhhhhhcccccchhhhhccccccccccchh MEM MMMMMMMMM SEQ DLRALARNLIFGLITCLTGVLGVGLGVEISRRLRHSNPRADPLVCATGLLGSAPFLFLSL SEG xxxxxxxxxxxxxxxx PRD hhhhhhhhhhhhheeeecccceeehhhhhhhhhhccccccceeecccceeeecccceeec MEM MMMMMMMMMMMMMMMMMMMMMMMMM SEQ ACARGSIVATYIFIFIGETLLSMNWAIVADILLYVVIPTRRSTAEAFQIVLSHLLGDAGS SEG PRD ccccchhhhheeeeeeccccccccchhhhhhheeeeeccccchhhhhhcccccccccccc MEM MMMM MMMMMMMMMMMMMMMMMMMMM MMMMMMMMMMMMM SEQ PYLIGLISDRLRRNWPPSFLSEFRALQFSLMLCAFVGALGGAAFLGTAIFIEADRRRAQL SEG xxxxxxxxxxxxx PRD ceeehhhhhhhhcccccchhhhhhhhhhhhhhhhhhhhhhhccccceeeeeehhhhhhhh MEM MMMMMMMM MM SEQ HVQGLLHEAGSTDDRIVVPQRGRSTRVPVASVLI SEG PRD hhhhhhhhccccceeeeeeccccccceeeeeccc MEM MMMMMMMMMMMMMMMMMMMMMMMMMMM Prosite for DKFZphmcfl_lel5.1 PS00002 177->181 GLYCOSAMINOGLYCAN PDOC00002 PS00004 340->344 CAMP_PHOSPHO_SITE PDOC00004 PS00005 270->273 PKC_PHOSPHO_SITE PDOC00005 PS00005 339->342 PKC_PHOSPHO_SITE PDOC00005 PS00005 368->371 PKC_PHOSPHO_SITE PDOC00005 PS00005 444->447 PKC_PHOSPHO_SITE PDOC00005 PS00006 11->15 CK2_PHOSPHO_SITE PDOC00006 PS00006 342->346 CK2_PHOSPHO_SITE PDOC00006 PS00006 431->435 CK2_PHOΞPHO_SITE PDOC00006 PS00008 26->32 MYRISTYL PDOC00008 PS00008 32->38 MYRISTYL PDOC00008 PS00008 52->58 MYRISTYL PDOC00008 PS00008 139->145 MYRISTYL PDOC00008 PS00008 176->182 MYRISTYL PDOC00008 PS00008 252->258 MYRISTYL PDOC00008 PS00008 262->268 MYRISTYL PDOC00008 PS00008 266->272 MYRISTYL PDOC00008 PS00008 288->294 MYRISTYL PDOC00008 PS00008 305->311 MYRISTYL PDOC00008 PS00008 397->403 MYRISTYL PDOC00008 PΞ00013 292->303 PROKAR LIPOPROTEIN PDOC00013 (No Pfam data available for DKFZphmcfl_lel5.1) DKFZphmcfl_lgl3 group: mammary carcinoma derived DKFZphmcfl_lgl3 encodes a novel 573 amino acid protein with very weak similarity to the human KIAA0543 protein and Musca domestica hermes transposase. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of mammary carcinoma- specific genes. similarity to KIAA0766 commplete cDNA, complete eds, few EST hits on genomic level encoded by AC005020, no splicing, genomic? Sequenced by DKFZ Locus : unknown Insert length: 2210 bp Poly A stretch at pos. 2200, polyadenylation signal at pos. 2176 1 GAAACCTGAT CTCATAAAAC CTAGGTCACA AAGGACAGCC CTGCAAAACA 51 GACCCTATTT GGATCAAGTG AGCCAGTTCC TGGAACCTGA ATAATGACTC 101 CTGAATCAAG GGATACTACA GATTTGTCTC CAGGGGGTAC CCAGGAGATG 151 GAAGGCATCG TGATAGTGAA GGTGGAGGAG GAAGATGAAG AAGACCATTT 201 TCAAAAGGAA AGAAACAAAG TAGAGTCATC GCCACAAGTT CTCAGTCGCT 251 CTACAACTAT GAATGAGAGA GCCTTATTGT CATCGTATTT AGTTGCATAT 301 AGAGTGGCAA AAGAGAAAAT GGCTCACACA GCGGCTGAAA AAATTATCCT 351 TCCAGCATGT ATGGACATGG TACGGACAAT TTTTGATGAC AAATCAGCTG 401 ATAAACTAAG AACTATACCT CTTAGTGATA ATACAATATC TCGTCGAATC 451 TGTACGATTG CAAAACATTT GGAAGCAATG CTTATTACAC GGCTGCAGTC 501 CGGTATAGAC TTTGCAATCC AACTCGATGA GAGCACTGAT ATTGCAAGTT 551 GTCCCACACT CTTGGTTTAT GTCAGATATG TGTGGCAAGA TGATTTTGTA 601 GAGGATCTCT TATGTTGTTT AAATTTAAAT TCACATATAA CTGGATTAGA 651 TTTATTTACT GAATTAGAAA ACTGCCTTCT TGGTCAGTAT AAATTAAACT 701 GGAAACATTG TAAAGGAATT TCAAGTGATG GAACAGCAAA TATGACCGGA 751 AAACACAGCA GACTTACTGA AAAATTGTTA GAAGCAACCC ACAACAATGC 801 TGTTTGGAAT CACTGTTTTA TTCATCGAGA AGCTTTGGTA TCCAAAGAAA 851 TTTCACCAAG TCTGATGGAT GTATTGAAAA ATGCAGTGAA AACTGTTAAT 901 TTTATTAAAG GAAGCTCACT GAATAGCCGA CTTCTCGAAA TATTTTGTTC 951 AGAGATTGGA GTGAACCACA CCCACTTATT GTTTCATACA GAAGTTCGTT 1001 GGCTTTCTCA AGGAAAAGTA TTGAGCAGAG TATATGAACT CAGGAACGAG 1051 ATTTACATTT TTCTCGTTGA AAAGCAATCT CATTTGGCAA ATATTTTTGA 1101 AGACGACATT TGGGTAACAA AATTGGCATA TTTAAGTGAT ATTTTTGGCA 1151 TTCTTAATGA ATTAAGCCTG AAAATGCAGG GGAAAAACAA TGATATATTT 1201 CAGTATCTTG AACATATTCT AGGATTCCAA AAGACGTTAT TATTGTGGCA 1251 AGCAAGACTT AAAAGTAACC GCCCTAGCTA CTATATGTTT CCAACATTAT 1301 TGCAACACAT CGAAGAGAAC ATTATTAATG AAGACTGCTT AAAAGAAATA 1351 AAA AGAGA TATTGTTGCA TCTCACTTCT TTGTCTCAAA CTTTTAATTA 1401 TTACTTTCCG GAAGAGAAAT TTGAATCATT AAAGGAAAAT ATTTGGATGA 1451 AAGATCCATT TGCTTTTCAA AACCCAGAAT CAATAATTGA GTTAAACTTG 1501 GAGCCTGAAG AAGAGAATGA ATTATTGCAG CTCAGTTCAT CATTCACACT 1551 AAAGAATTAT TATAAGATAT TAAGTTTATC AGCATTTTGG ATTAAGATTA 1601 AAGATGACTT TCCACTGCTA AGTAGGAAGA GTATATTGCT GTTACTACCA 1651 TTCACAACTA CATATTTGTG TGAACTAGGA TTTTCAATCT TGACACGGTT 1701 AAAAACAAAG AAGAGAAATA GGCTCAATAG TGCACCAGAT ATGCGGGTAG 1751 CATTATCTTC ATGTGTTCCT GACTGGAAGG AACTTATGAA CAGACAAGCA 1801 CACCCATCAC ATTAAATACA AACTTTACAA AATTCTGTGT ATAGCCAGGT 1851 GTGGTGGCTT ACGCCTGTAA TCCCAGCAGT GGGAGACCGA GGTGGGCAGA 1901 TCACTTGAGT TCAAGACCAG CCTGGCCAAC ATGGTGAAAC CCCATCTCTA 1951 CTAAAAATAG AAACCTTAGC CAGGCGTGGT GGCACATGCC TGCAGTCCCA 2001 GTTACTTGGG TGCCTGAGGC AGGAGAATCT CTTAAACCAG GAAGGCAGAG 2051 ATTGCAGTGA GCTGAGATAA TCCCACTGCA TTCCAGCCTG GGCAACAGCG 2101 TGAGACTTCA TCTCAAAAAA AAAAAATTGT ATTTGTACTT TTAAAGGGAT 2151 TTTGCAGTAT GTTGTAGTTA AACGTTAATA AAATTATATT TGTAATTAGG 2201 AAAAAAAAAA BLAST Results Entry AC005020 from database EMBL: Homo sapiens clone GS259H13; HTGS phase 1, 4 unordered pieces. Score = 9110, P = O.Oe+00, identities = 1822/1822 Medline entries No Medline entry Peptide information for frame 1 ORF from 94 bp to 1812 bp; peptide length: 573 Category: similarity to unknown protein 1 MTPESRDTTD LSPGGTQEME GIVIVKVEEE DEEDHFQKER NKVESSPQVL 51 SRSTTMNERA LLSSYLVAYR VAKEKMAHTA AEKIILPACM DMVRTIFDDK 101 SADKLRTIPL SDNTISRRIC TIAKHLEAML ITRLQSGIDF AIQLDESTDI 151 ASCPTLLVYV RYVWQDDFVE DLLCCLNLNS HITGLDLFTE LENCLLGQYK 201 LNWKHCKGIS SDGTANMTGK HSRLTEKLLE ATHNNAVWNH CFIHREALVS 251 KEISPSLMDV LKNAVKTVNF IKGSSLNSRL LEIFCSEIGV NHTHLLFHTE 301 VRWLSQGKVL SRVYELRNEI YIFLVEKQSH LANIFEDDIW VTKLAYLSDI 351 FGILNELSLK MQGKNNDIFQ YLEHILGFQK TLLLWQARLK SNRPSYYMFP 401 TLLQHIEENI INEDCLKEIK LEILLHLTSL SQTFNYYFPE EKFESLKENI 451 WMKDPFAFQN PESIIELNLE PEEENELLQL SSSFTLKNYY KILΞLSAFWI 501 KIKDDFPLLS RKSILLLLPF TTTYLCELGF SILTRLKTKK RNRLNSAPDM 551 RVALSSCVPD WKELMNRQAH PSH BLASTP hits Entry AC004877_3 from database TREMBLNEW: gene: "WUGSC :H_DJ0751H13.2"; product: "KIAA0543 protein"; Homo sapiens PAC clone DJ0751H13 from 7q35-qter, complete sequence. Score = 86, P = 4.4e-03, identities = 46/179, positives = 78/179 Entry MD36211_1 from database TREMBL: product: "Hermes transposase"; Musca domestica Hermes transposase gene, complete eds. Score = 105, P = 3.0e-02, identities = 101/465, positives = 202/465 Alert BLASTP hits for DKFZphmcfl_lgl3, frame 1 TREMBL :AB018309_1 gene: "KIAA0766"; product: "KIAA0766 protein"; Homo sapiens mRNA for KIAA0766 protein, complete eds., N = 1, Score = 300, P = l.le-23 >TREMBL:AB018309_1 gene: "KIAA0766"; product: "KIAA0766 protein"; Homo sapiens mRNA for KIAA0766 protein, complete eds. Length = 607 HSPs: Score = 300 (45.0 bits), Expect = l.le-23, P = l.le-23 Identities = 120/485 (24%), Positives = 229/485 (47%) Query: 89 CMD-MVRTIFDDKSADKLRTIPLSDNTISRRICTIAKHLEAMLITRLQSGIDFAIQLDES 147 CM+ ++R + + L+ + LS + +RI +1 ++L L R + +++ LD+ Sbjct: 124 CMEVLLREVLPEH-VSVLQGVDLSPDITRQRILSIDRNLRNQLFNRARDFKAYSLALDDQ 182 Query: 148 TDIASCPTLLVYVRYVWQD-DFVEDLLCCLNLNSHIT-GLDLFTELENCLLGQYKLNWKH 205 +A LLV++R V + + EDLL +NL H + G + LE+ L L+ + Sbjct: 183 AFVAYENYLLVFIRGVGPELEVQEDLLTIINLTHHFSVGALMSAILES—LQTAGLSLQR 240 Query: 206 CKGISSDGTANMTGKHSRLTEKLLEATHNNAVWN—HC—FIHREALVSKEISPSLMDVL 261 G+++ T M G++S L + E + WN H F+H E L S ++ + ++ Sbjct: 241 MVGLTTTHTLRMIGENSGLVSYMREKAVSPNCWNVIHYSGFLHLELLSSYDVDVN—QII 298 Query: 262 KNAVKTVNFIKGSSLNSRLLEIFCSEIGVNHTHLLFHTEVR-WLSQGKVLSRVYELRNEI 320 + + IK + + +E H + + WL +GK L ++ LR E+ Sbjct: 299 NTISEWIVLIKTRGVRRPEFQTLLTESESEHGERVNGRCLNNWLRRGKTLKLIFSLRKEM 358 Query: 321 YIFLVEKQSHLANIFEDDIWVTKLAYLSDIFGILNELSLKMQGKNNDIFQYLEHILGFQK 380 FLV + + F D W+ +L Dl L ELS +++ +HI F+ Sb j ct: 359 EAFLVSVGATTVH-FSDKQWLCDFGFLVDIMEHLRELSEELRVSKVFAAAAFDHICTFEV 417 Query: 381 TLLLWQARLKSNRPSYYMFPTLLQHIEE NIINEDCLKEIKLEILLHLTSLSQTFNY 436 L L+Q ++ + FP L + ++E N +E + ++++ L + F Sbjct: 418 KLNLFQRHIEEKNLTD—FPALREVVDELKQQNKEDEKIFDPDRYQMVI—CRLQKEFER 473 Query: 437 YFPEEKFESLKENIWM-KDPFAFQNPESIIELNLEPEEENELLQLSSSFTLKNYYKILSL 495 +F + +F +K+++ + +PF F+ + I + +E L +L ++ L N Y+I L Sbjct: 474 HFKDLRF—IKKDLELFSNPFNFKPEYAPISVRVE LTKLQANTNLWNEYRIKDL 525 Query: 496 SAFWIKIK-DDFPLLSRKSILLLLPFTTTYLCELGFSILTRLKTKKRNRLNSA PDMR 551 F+ + + +P++ + + F + +CE FS LTR + L R Sbjct: 526 GQFYAGLSAESYPIIKGVACKVASLFDSNQICEKAFSYLTRNQHTLSQPLTDEHLQALFR 585 Query: 552 VALSSCVPDWKELMNRQAHPSH 573 VA + P W +L+ R+ + S+ Sbjct: 586 VATTEMEPGWDDLV-RERNESN 606 Score : = 290 (43.5 bits), Expect = 1.5e-22, P = 1.5e-22 Identities : = 120/485 (24%), Positives = 228/485 (47%) Query: 89 CMD-MVRTIFDDKSADKLRTIPLSDNTISRRICTIAKHLEAMLITRLQSGIDFAIQLDES 147 CM+ ++R + + L+ + LS + +RI +1 ++L L R + +++ LD+ Sbjct: 124 CMEVLLREVLPEH-VSVLQGVDLSPDITRQRILSIDRNLRNQLFNRARDFKAYSLALDDQ 182 Query: 148 TDIASCPTLLVYVRYVWQD-DFVEDLLCCLNLNSHIT-GLDLFTELENCLLGQYKLNWKH 205 +A LLV++R V + + EDLL +NL H + G + LE+ L L+ + Sbjct: 183 AFVAYENYLLVFIRGVGPELEVQEDLLTIINLTHHFSVGALMΞAILES—LQTAGLSLQR 240 Query: 206 CKGISSDGTANMTGKHSRLTEKLLEATHNNAVWNHCFIHREALVSKEISPSLMDV-LKNA 264 G+++ T M G++S L + E + WN IH + E+ S DV + Sbjct: 241 MVGLTTTHTLRMIGENSGLVSYMREKAVSPNCW —VIHYSGFLHLELLSSY-DVDVNQI 297 Query: 265 VKTVN FIKGSSLNSRLLEIFCSEIGVNHTHLLFHTEVR-WLSQGKVLSRVYELRNE 319 + T++ IK + + +E H + + WL +GK L ++ LR E Sbjct: 298 INTISEWIVLIKTRGVRRPEFQTLLTESESEHGERVNGRCLNNWLRRGKTLKLIFSLRKE 357 Query: 320 IYIFLVEKQSHLANIFEDDIWVTKLAYLSDIFGILNELSLKMQGKNNDIFQYLEHILGFQ 379 + FLV + + F D W+ +L Dl L ELS +++ +HI F+ Sbjct: 358 MEAFLVSVGATTVH-FSDKQWLCDFGFLVDIMEHLRELSEELRVSKVFAAAAFDHICTFE 416 Query: 380 KTLLLWQARLKSNRPSYYMFPTLLQHIEENIINEDCLKEIKL EILLHLTSLSQTFN 435 L L+Q ++ + FP L + ++E + + ++ K+ + + L + F Sbjct: 417 VKLNLFQRHIEEKNLTD—FPALREVVDE—LKQQNKEDEKIFDPDRYQMVICRLQKEFE 472 Query: 436 YYFPEEKFESLKENIWM-KDPFAFQNPESIIELNLEPEEENELLQLSSSFTLKNYYKILS 494 +F + +F +K+++ + +PF F+ + I + +E L +L ++ L N Y+I Sbjct: 473 RHFKDLRF—IKKDLELFSNPFNFKPEYAPISVRVE LTKLQANTNLWNEYRIKD 524 Query: 495 LSAFWIKIK-DDFPLLSRKSILLLLPFTTTYLCELGFSILTRLKTKKRNRLNSA PDM 550 L F+ + + +P++ + + F + +CE FS LTR + L Sbjct: 525 LGQFYAGLSAESYPIIKGVACKVASLFDSNQICEKAFSYLTRNQHTLSQPLTDEHLQALF 584 Query: 551 RVALSSCVPDWKELMNRQAHPSH 573 RVA + P W +L+ R+ + S+ Sbjct: 585 RVATTEMEPGWDDLV-RERNESN 606 Pedant information for DKFZphmcfl_lgl3, frame 1 Report for DKFZphmcf1 lgl3.1 [LENGTH] 573 [MW] 66276.85 [pi] 5.82 [HOMOL] TREMBL :AB018309_1 gene: "KIAA0766" product: "KIAA0766 protein"; Homo sapiens mRNA for KIAA0766 protein, complete eds. le-18 [PROSITE] MYRISTYL 3 [PROSITE] CK2_PHOSPHO_SITE 10 [PROSITE] TYR_PHOSPHO_SITE 1 [PROSITE] PKC_PHOSPHO_SITE 9 [PROSITE] AΞN_GLYCOSYLATION 2 [KW] All_Alpha [KW] LOW COMPLEXITY 8.90 % SEQ MTPESRDTTDLSPGGTQEMEGIVIVKVEEEDEEDHFQKERNKVESSPQVLSRSTTMNERA SEG xxxxxxx PRD ccccccccccccccccccceeeeeeeeccccchhhhhhhhhhcccccceeecccchhhhh SEQ LLSSYLVAYRVAKEKMAHTAAEKI ILPACMDMVRTI FDDKSADKLRTI PLSDNTISRRIC SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhchhhhhhhhhhcccccceeeeecccchhhhhhh SEQ TIAKHLEAMLITRLQSGIDFAIQLDESTDIASCPTLLVYVRYVWQDDFVEDLLCCLNLNS SEG PRD hhhhhhhhhhhhhhhhhheeeccccccccccccccceeeeeeeccchhhhhhhhhhccce SEQ HITGLDLFTELENCLLGQYKLNWKHCKGISSDGTANMTGKHSRLTEKLLEATHNNAVWNH SEG PRD eeeehhhhhhhhhhhhhhhccccccccccccccceeeecccchhhhhhhhhhccccceee SEQ CFIHREALVSKEISPSLMDVLKNAVKTVNFIKGSSLNSRLLEIFCSEIGVNHTHLLFHTE SEG PRD hhhhhhhhhhhhcccchhhhhhhhhhhheeecccccchhhhhhhhhhccccchhhhhhhh SEQ VRWLSQGKVLSRVYELRNEIYIFLVEKQSHLANIFEDDIWVTKLAYLSDIFGILNELSLK SEG PRD cccccccchhhhhhhhhhhhhhhhhhhhchhhhhcccceeehhhhhhhhhhhhhhhhhhh SEQ MQGKNNDIFQYLEHILGFQKTLLLWQARLKSNRPSYYMFPTLLQHIEENIINEDCLKEIK SEG xxxxx PRD hhccccccchhhhhhhhhhhhhhhhhhhhhcccccccccchhhhhhhhhhhhcchhhhhh SEQ LEILLHLTSLSQTFNYYFPEEKFESLKENIWMKDPFAFQNPEΞIIELNLEPEEENELLQL SEG xxxxx xxxxxxxxxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhccchhhhhhhhhhhhhcccccccccccceeecccchhhhhhhhh SEQ SSSFTLKNYYKILSLSAFWIKIKDDFPLLSRKSILLLLPFTTTYLCELGFSILTRLKTKK SEG XXX xxxxxxxxxxx PRD hhcccchhhhhhhhhhhhhcccccccccchhhhhhhhhccceeeeehhhhhhhhhhhhhh SEQ RNRLNSAPDMRVALSSCVPDWKELMNRQAHPSH SEG PRD hcccccccccceeeccccccchhhhhhhccccc Prosite for DKFZphmcfl_lgl3.1 PS00001 216->220 ASN_GLYCOSYLATION PDOC00001 PS00001 291->295 AΞN_GLYCOSYLATION PDOC00001 PS00005 116->119 PKC_PHOSPHO_SITE PDOC00005 PS00005 218->221 PKC_PHOSPHO_SITE PDOC00005 PS00005 225->228 PKC_PHOSPHO_SITE PDOC00005 PS00005 358->361 PKC_PHOSPHO_SITE PDOC00005 PS00005 391->394 PKC_PHOSPHO_SITE PDOC00005 PS00005 445->448 PKC_PHOSPHO_SITE PDOC00005 PS00005 485->488 PKC_PHOSPHO_SITE PDOC00005 PS00005 510->513 PKC_PHOSPHO_SITE PDOC00005 PS00005 538->541 PKC_PHOSPHO_SITE PDOC00005 PS00006 55->59 CK2_PHOSPHO_SITE PDOC00006 PS00006 79->83 CK2_PHOSPHO_SITE PDOC00006 PS00006 95->99 CK2_PHOSPHO_SITE PDOC00006 PS00006 136->140 CK2_PHOSPHO_SITE PDOC00006 PS00006 183->187 CK2_PHOSPHO_SITE PDOC00006 PS00006 189->193 CK2_PHOSPHO_SITE PDOC00006 PS00006 256->260 CK2_PHOSPHO_SITE PDOC00006 PS00006 445->449 CK2_PHOSPHO_SITE PDOC00006 PS00006 463->467 CK2_PHOSPHO_SITE PDOC00006 PS00006 546->550 CK2_PHOSPHO_SITE PDOC00006 PS00007 364->372 TYR_PHOSPHO_SITE PDOC00007 PS00008 137->143 MYRISTYL PDOC00008 PS00008 273->279 MYRISTYL PDOC00008 PS00008 289->295 MYRISTYL PDOC00008 (No Pfam data available for DKFZphmcfl_lgl3.1) DKFZphtes3_14g5 group: testes derived DKFZphtes3_14g5 encodes a novel 379 amino acid protein with strong similarity to murine cell growth regulating nucleolar protein LYAR. The novel protein is very similar to murine Ly-1 antibody reactive clone protein (LYAR) . It contains a ATP/GTP-binding site motif A (P-loop, interacts with one of the phosphate groups of a ATP/GTP nucleotide), but not the zinc finger motif and and nuclear localization signals of lyar . No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . strong similarity to cell growth regulating nucleolar protein LYAR, of mouse complete cDNA, complete eds, EST hits Sequenced by BMFZ Locus: unknown Insert length: 1503 bp Poly A stretch at pos. 1467, polyadenylation signal at pos. 1440 1 CCCAGAGGTC CGACCTGGGA GGCTGGGGCT CAGAGAGCAA TGTTTGCTGT 51 CTTCCATTGG AGTGACTGAA TTTCTACATG ACGGCTTTTT GACAAGACTT 101 AAAACCTGTC TTGGATAGAG AATATTTAGC CATTTACCTA AAAATGGTAT 151 TTTTTACATG CAATGCATGT GGTGAATCAG TGAAGAAAAT ACAAGTGGAA 201 AAGCATGTGT CTGTTTGCAG AAACTGTGAA TGCCTTTCTT GCATTGACTG 251 CGGTAAAGAT TTCTGGGGCG ATGACTATAA AAACCACGTG AAATGCATAA 301 GTGAAGATCA GAAGTATGGT GGCAAAGGCT ATGAAGGTAA AACCCACAAA 351 GGCGACATCA AACAGCAGGC GTGGATTCAG AAAATTAGTG AATTAATAAA 401 GAGACCCAAT GTCAGCCCCA AAGTGAGAGA ACTTTTAGAG CAAATTAGTG 451 CTTTTGACAA CGTTCCCAGG AAAAAGGCAA AATTTCAGAA TTGGATGAAG 501 AACAGTTTAA AAGTTCATAA TGAATCCATT CTGGACCAGG TGTGGAATAT 551 CTTTTCTGAA GCTTCCAACA GCGAACCAGT CAATAAGGAA CAGGATCAAC 601 GGCCACTCCA CCCAGTGGCA AATCCACATG CAGAAATCTC CACCAAGGTT 651 CCAGCCTCCA AAGTGAAAGA CGCCGTGGAA CAGCAAGGGG AGGTGAAGAA 701 GAATAAAAGA GAAAGAAAGG AAGAACGGCA GAAGAAAAGG AAAAGAGAAA 751 AGAAAGAACT AAAGTTAGAA AACCACCAGG AAAACTCAAG GAATCAGAAG 801 CCTAAGAAGC GCAAAAAGGG ACAGGAGGCT GACCTTGAGG CTGGTGGGGA 851 GGAAGTCCCT GAGGCCAATG GCTCTGCAGG GAAGAGGAGC AAGAAGAAGA 901 AGCAGCGCAA GGACAGCGCC AGTGAGGAAG AGGCACGCGT GGGCGCAGGG 951 AAGAGGAAGC GGAGGCACTC GGAAGTTGAA ACAGATTCTA AGAAGAAAAA 1001 GATGAAGCTC CCAGAGCATC CTGAGGGCGG AGAACCAGAA GACGATGAGG 1051 CTCCTGCAAA AGGTAAATTC AACTGGAAGG GAACTATTAA AGCAATTCTG 1101 AAACAGGCCC CAGACAATGA AATAACCATC AAAAAGCTAA GGAAAAAGGT 1151 TTTAGCTCAG TACTACACAG TGACAGATGA GCATCACAGA TCCGAAGAGG 1201 AACTCCTGGT CATCTTTAAC AAGAAAATCA GCAAGAACCC TACCTTTAAG 1251 TTATTAAAGG ACAAAGTCAA GCTTGTGAAA TGAACATTTG TGTATTTAAA 1301 AATTGAATCC ATTCTGCTGA CTTCTTCCTT TCACTGCTGT TTATAAAATG 1351 TGTAATGAAT TCTAACAACT CAAATTTTGC TTTTTGAAGC TGTATTTTTA 1401 AGTTAAGAAA ATATATTTTT GGTATAACTT TTATGAGAAA AATAAAATAT 1451 ATTCTGGTCC AAACTTCAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 1501 AAA BLAST Results No BLAST result Medline entries 93259460: LYAR, a novel nucleolar protein with zinc fmger DNA-bindmg motifs, is involved in cell growth regulation. Peptide information for frame 3 ORF from 144 bp to 1280 bp; peptide length: 379 Category: strong similarity to known protein Classification: Cell division Prosite motifs: ATP GTP A (60-68) 1 MVFFTCNACG ESVKKIQVEK HVSVCRNCEC LΞCIDCGKDF WGDDYKNHVK 51 CISEDQKYGG KGYEGKTHKG DIKQQAWIQK ISELIKRPNV SPKVRELLEQ 101 ISAFDNVPRK KAKFQNWMKN SLKVHNESIL DQVWNIFSEA SNSEPVNKEQ 151 DQRPLHPVAN PHAEISTKVP ASKVKDAVEQ QGEVKKNKRE RKEERQKKRK 201 REKKELKLEN HQENSRNQKP KKRKKGQEAD LEAGGEEVPE ANGSAGKRSK 251 KKKQRKDSAS EEEARVGAGK RKRRHSEVET DSKKKKMKLP EHPEGGEPED 301 DEAPAKGKFN WKGTIKAILK QAPDNEITIK KLRKKVLAQY YTVTDEHHRS 351 EEELLVIFNK KIΞKNPTFKL LKDKVKLVK BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_14g5, frame 3 PIR:A40683 cell growth regulating nucleolar protein LYAR - mouse, N = 1, Score = 1410, P = 2.7e-144 SWISSPROT :YQ58_CAEEL HYPOTHETICAL 28.5 KD PROTEIN C16C10.8 IN CHROMOSOME III., N = 1, Score = 381, P = 2.9e-35 TREMBL:AC003058_18 gene: "F27F23.18"; product: "putative RNA-bindmg protein"; Arabidopsis thaliana chromosome II BAC F27F23 genomic sequence, complete sequence., N = 3, Score = 139, P = 4e-15 PIR:S70049 nucleic acid-bmding protein YCR087c-a - yeast (Saccharomyces cerevisiae), N = 1, Score = 164, P = 1.4e-ll >PIR:A40683 cell growth regulating nucleolar protein LYAR - mouse Length = 388 HSPs: Score = 1410 (211.6 bits), Expect = 2.7e-144, P = 2.7e-144 Identities = 275/388 (70%), Positives = 317/388 (81%) Query: 1 MVFFTCNACGESVKKIQVEKHVSVCRNCECLSCIDCGKDFWGDDYKNHVKCISEDQKYGG 60 MVFFTCNACGESVKKIQVEK VS CRNCECLSCIDCGKDFWGDDYK+HVKCISE QKYGG Sbjct: 1 MVFFTCNACGESVKKIQVEKQVSNCRNCECLSCIDCGKDFWGDDYKSHVKCISEGQKYGG 60 Query: 61 KGYEGKTHKGDIKQQAWIQKISELIKRPNVSPKVRELLEQISAFDNVPRKKAKFQNWMKN 120 KGYE KTHKGD KQQAWIQKI+ELIK+PNVSPKVRELL+QISAFDNVP KKAKFQNWMKN Sbjct: 61 KGYEAKTHKGDAKQQAWIQKINELIKKPNVSPKVRELLQQISAFDNVPIKKAKFQNWMKN 120 Query: 121 ΞLKVHNESILDQVWNIFSEASNSEPVNKEQDQRPLHPVANPHAEIS-TKVPASKVKDAVE 179 SLKVH++S+L+QVW+IFSEAS+SE ++Q Q P H A PHAE+ TKVP++K E Sbjct: 121 SLKVHSDSVLEQVWDIFSEASSSE QDQQQPPSH-TAKPHAEMPITKVPSAKTNGTTE 176 Query: 180 QQGEVKKNKRERKEERQKKRKREKKELKLENHQENSRNQKPKKRKKGQEADLEAGGEEVP 239 +Q E KKNKRERKEERQK RK+EKKELKLENHQEN R QKPKKRKK QEA EA GE+ Sbjct: 177 EQTEAKKNKRERKEERQKNRKKEKKELKLENHQENLRGQKPKKRKKNQEAGHEAAGEDGA 236 Query: 240 EANG SAGKRSKKKKQRKDSASEEEA RVGAGKRKR-RHSEVETDSKKKKM 287 + +G G+ S++ R E+ A + AGKRKR +HS E+ KKKKM Sbjct: 237 DGSGPPEKKKAQGGQASEEGADRNGGPGEDRAEGQTKTAAGKRKRPKHSGAESGYKKKKM 296 Query: 288 KLPEHPEGGEPEDDEAPAKGKFNWKGTIKAILKQAPDNEITIKKLRKKVLAQYYTVTDEH 347 KLPE PE GE +D EAP+KGKFNWKGTIKA+LKQAPDNEI++KKL+KKV+AQY+ V ++ Sbjct: 297 KLPEQPEEGEAKDHEAPSKGKFNWKGTIKAVLKQAPDNEISVKKLKKKVIAQYHAVMNDT 356 Query: 348 HRSEEELLVIFNKKISKNPTFKLLKDKVKLVK 379 EEELL IFN+KIS+NPTFK+LKD+VKL+K Sbjct: 357 SHHEEELLAIFNRKISRNPTFKVLKDRVKLLK 388 Pedant information for DKFZphtes3_14g5, frame 3 Report for DKFZphtes3_14g5 . 3 [ LENGTH ] 379 [MW ] 43634 . 03 [pi ] 9 . 59 [HOMOL] PIR:A40683 cell growth regulating nucleolar protein LYAR - mouse le-122 [FUNCAT] 04.99 other transcription activities [S. cerevisiae, YCR087c-a] 2e-ll [BLOCKS] BL00603D Thymidine kinase cellular-type proteins [BLOCKS] BL00530C [PROSITE] ATP_GTP_A 1 [KW] All_Alpha [KW] LOW_COMPLEXITY 18.73 % SEQ MVFFTCNACGESVKKIQVEKHVSVCRNCECLSCIDCGKDFWGDDYKNHVKCISEDQKYGG SEG PRD ccccccccccccchhhhhhhheeecccccceeeccccccccccccccceeeeeccccccc SEQ KGYEGKTHKGDIKQQAWIQKISELIKRPNVSPKVRELLEQISAFDNVPRKKAKFQNWMKN SEG PRD cccccccccchhhhhhhhhhhhhhhhhccchhhhhhhhhhhhcccccchhhhhhhhhhhc SEQ SLKVHNEΞILDQVWNIFSEASNSEPVNKEQDQRPLHPVANPHAEISTKVPASKVKDAVEQ SEG PRD cccccchhhhhhhhhhhhhhhcchhhhhhhhcccccccccccccceeecccccchhhhhh SEQ QGEVKKNKRERKEERQKKRKREKKELKLENHQENSRNQKPKKRKKGQEADLEAGGEEVPE SEG .... xxxxxxxxxxxxxxxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccccccchhhhhhchhhhhccccccc SEQ ANGSAGKRSKKKKQRKDSASEEEARVGAGKRKRRHSEVETDΞKKKKMKLPEHPEGGEPED SEG ..xxxxxxxxxxxxxxxxxx xxxxxxxxxxx PRD cccccccchhhhhhhhccchhhhhhhhhcccccccccccccchhhhhhcccccccccccc SEQ DEAPAKGKFNWKGTIKAILKQAPDNEITIKKLRKKVLAQYYTVTDEHHRSEEELLVIFNK SEG xxxxx PRD cccccceeeehhhhhhhhhhhccccccchhhhhhhhhhhhhhhccchhhhhhhhhhhhhh SEQ KISKNPTFKLLKDKVKLVK SEG xxxxxxxxxxx PRD ccccccchhhhhhhhhccc Prosite for DKFZphtes3_14g5.3 PS00017 60->68 ATP GTP A PDOC00017 (No Pfam data available for DKFZphtes3_14g5.3) DKFZphtes3 14h21 group: nucleic acid management DKFZphtes3_14h21 encodes a novel 648 amino acid protein with strong similarity to mus musculus RNA helicase and several RNA-dependent ATPases from the DEAD box family. RNA helicases comprise a large family of proteins that are involved in basic biological systems such as nuclear and mitochondrial splicing processes, RNA editing, rRNA processing, translation initiation, nuclear mRNA export, and mRNA degradation. RNA helicases are essential factors in cell development and differentiation, and some of them play a role in transcription and replication of viral single-stranded RNA genomes. The members of the largest subgroup, the DEAD and DEAH box proteins, exhibit a strong dependence of the unwinding activity on ATP hydrolysis. The novel protein contains a DEAD-box and a ATP/GTP-bindmg site motif A (P-loop) and is a new member of this subgroup. The new protein can find application in modulating RNA metabolism and gene expression. strong similarity to RNA helicases start at Bp 33 matches Kozak consensus ACNatg Sequenced by BMFZ Locus : unknown Insert length: 2200 bp Poly A stretch at pos. 2166, polyadenylation signal at pos. 2140 1 CAACGACGTC GGACGCGCCC CTTCTTGGAA CAATGTCCCA CCACGGAGGA 51 GCTCCCAAGG CCTCTACGTG GGTCGTTGCT AGTCGGCGAA GCTCGACAGT 101 GTCCCGAGCG CCAGAGAGGA GGCCGGCGGA GGAGTTGAAT CGAACAGGTC 151 CTGAGGGATA TAGTGTCGGC AGAGGTGGTC GCTGGAGAGG CACCTCTAGG 201 CCCCCGGAGG CCGTGGCCGC TGGTCACGAG GAACTGCCGC TGTGTTTTGC 251 TTTGAAGAGC CACTTTGTTG GCGCGGTAAT CGGTCGTGGT GGGTCAAAAA 301 TAAAGAATAT ACAAAGTACA ACAAACACCA CAATCCAAAT AATACAAGAA 351 CAACCAGAAT CATTAGTCAA AATTTTTGGC AGCAAGGCAA TGCAAACGAA 401 AGCAAAAGCA GTGATAGACA ATTTTGTTAA AAAGCTAGAA GAAAATTACA 451 ATTCAGAATG CGGAATTGAT ACTGCATTCC AACCTTCTGT TGGAAAAGAT 501 GGAAGCACAG ATAACAATGT TGTTGCAGGA GATCGGCCAT TGATAGATTG 551 GGATCAAATT AGAGAGGAAG GTTTGAAATG GCAAAAAACA AAGTGGGCAG 601 ATTTACCACC AATTAAGAAA AACTTTTATA AAGAGTCCAC TGCCACAAGT 651 GCCATGTCAA AAGTAGAAGC AGATAGTTGG AGGAAAGAAA ATTTTAATAT 701 AACGTGGGAT GACTTGAAGG ATGGGGAGAA ACGACCTATC CCCAATCCTA 751 CCTGCACATT TGATGACGCC TTTCAATGTT ATCCTGAGGT TATGGAAAAC 801 ATTAAAAAGG CAGGTTTTCA AAAGCCAACA CCTATTCAGT CACAGGCATG 851 GCCCATTGTG TTGCAAGGAA TAGATCTTAT AGGAGTAGCC CAGACTGGAA 901 CAGGAAAGAC ATTGTGTTAT TTAATGCCTG GATTTATTCA TCTGGTCCTT 951 CAACCCAGCC TTAAAGGTCA AAGGAATAGA CCCGGCATGT TAGTTCTAAC 1001 TCCCACTCGG GAATTAGCAC TTCAAGTAGA AGGAGAATGT TGCAAATATT 1051 CATATAAAGG GCTTCGGAGT GTTTGTGTAT ATGGTGGTGG AAATAGAGAT 1101 GAACAAATAG AAGAGCTTAA AAAAGGTGTA GATATCATAA TTGCAACTCC 1151 CGGAAGATTG AATGATCTGC AAATGAGTAA CTTCGTCAAT CTGAAGAATA 1201 TAACCTACTT GGTTTTAGAT GAAGCAGACA AGATGTTGGA CATGGGATTT 1251 GAACCCCAGA TAATGAAGAT TTTGTTAGAT GTGCGCCCAG ATAGGCAGAC 1301 AGTTATGACC AGTGCTACAT GGCCTCATTC AGTTCATCGC CTCGCACAAT 1351 CTTATTTGAA AGAACCAATG ATTGTCTATG TTGGTACATT GGATCTAGTT 1401 GCTGTAAGTT CAGTGAAGCA AAATATAATT GTAACCACCG AGGAAGAGAA 1451 ATGGAGTCAC ATGCAAACTT TTCTACAGAG TATGTCATCC ACAGACAAAG 1501 TCATTGTCTT CGTTTCTCGA AAAGCTGTTG CGGATCACTT ATCAAGTGAC 1551 CTAATACTTG GAAATATATC AGTAGAGTCT CTGCATGGAG ATAGAGAACA 1601 GAGAGATCGG GAGAAAGCAT TAGAGAACTT TAAAACAGGC AAAGTGAGAA 1651 TACTAATTGC AACTGATCTA GCCTCTAGAG GACTTGATGT CCATGACGTT 1701 ACACATGTCT ATAATTTTGA CTTTCCACGG AATATTGAAG AATACGTACA 1751 CCGAATAGGG CGCACGGGAA GAGCAGGGAG GACTGGTGTT TCCATTACAA 1801 CTTTGACTAG AAATGATTGG AGGGTTGCCT CTGAATTGAT TAATATTCTG 1851 GAAAGAGCAA ATCAGAGTAT TCCAGAGGAG CTTGTATCAA TGGCTGAGAG 1901 GTTTGAGGCA CATCAACGGA AAAGGGAAAT GGAAAGAAAA ATGGAAAGAC 1951 CTCAAGGAAG GCCCAAGAAG TTTCATTAAT GTCTTCTGTA CTAGTGGGGT 2001 AGAGAATTCA AGATTTTTTA GAAATATAGT AAGACAGAAG TATTGGACAT 2051 GTTGGCAGTA TGAAGAGACC GGACTGATTT GACTGATTCT TAAAATAATA 2101 GTGTTTGAAA ATATAGAATC CAGTGTTTTA TACTTTCTTT AATAAAAATA 2151 GAAGTATTTA AACTTGAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 3 ORF from 33 bp to 1976 bp; peptide length: 64£ Category: strong similarity to known protein Classification: Nucleic acid management Prosite motifs: ATP_GTP_A (286-294) DEAD ATP HELICASE (394-403) 1 MSHHGGAPKA STWVVASRRS STVSRAPERR PAEELNRTGP EGYSVGRGGR 51 WRGTSRPPEA VAAGHEELPL CFALKSHFVG AVIGRGGSKI KNIQSTTNTT 101 IQIIQEQPES LVKIFGSKAM QTKAKAVIDN FVKKLEENYN SECGIDTAFQ 151 PSVGKDGSTD NNVVAGDRPL IDWDQIREEG LKWQKTKWAD LPPIKKNFYK 201 ESTATSAMSK VEADSWRKEN FNITWDDLKD GEKRPIPNPT CTFDDAFQCY 251 PEVMENIKKA GFQKPTPIQS QAWPIVLQGI DLIGVAQTGT GKTLCYLMPG 301 FIHLVLQPSL KGQRNRPGML VLTPTRELAL QVEGECCKYS YKGLRΞVCVY 351 GGGNRDEQIE ELKKGVDIII ATPGRLNDLQ MSNFVNLKNI TYLVLDEADK 401 MLDMGFEPQI MKILLDVRPD RQTVMTSATW PHSVHRLAQS YLKEPMIVYV 451 GTLDLVAVSS VKQNIIVTTE EEKWSHMQTF LQSMSSTDKV IVFVSRKAVA 501 DHLSSDLILG NISVESLHGD REQRDREKAL ENFKTGKVRI LIATDLASRG 551 LDVHDVTHVY NFDFPRNIEE YVHRIGRTGR AGRTGVSITT LTRNDWRVAS 601 ELINILERAN QSIPEELVSM AERFEAHQRK REMERKMERP QGRPKKFH BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_14h21, frame 3 TREMBL :CEY54G11A_9 gene: "Y54G11A.3"; Caenorhabditis elegans cosmid Y54G11A, N = 1, Score = 1008, P = 1. le-101 TREMBL :SPBP8B7_16 gene: "dbp2"; "SPBP8B7.16c"; product: "p68-lιke protein."; S. pombe chromosome II pi p8B7., N = 1, Score = 971, P = 9.1e-98 PIR:S13757 RNA helicase DBP2 - yeast (Saccharomyces cerevisiae), N = 1, Score = 970, P = 1.2e-97 PIR:S14048 RNA helicase dbp2 - fission yeast (Schizosaccharomyces pombe), N = 1, Score = 961, P = le-96 PIR:A57514 RNA helicase HEL117 - rat, N = 2, Score = 888, P = 7.8e-91 >TREMBL:CEY54G11A_9 gene: "Y54G11A.3"; Caenorhabditis elegans cosmid Y54G11A Length = 504 HSPs: Score = 1008 (151.2 bits), Expect = 1. le-101, P = 1. le-101 Identities = 211/473 (44%), Positives = 298/473 (63%) Query: 174 DQIREEGLKWQKTKWADLPPIKKNFYKESTATSAMSKVEADSWRKENFNITWDDLKDGEK 233 D++++E W K PI ++ YK +S + + ++ Sbjct: 23 DRLKDENFSWMK PIVRDLYKIPNEQKNLSPEQLQELYTNGGVMKVYPFREEST 75 Query: 234 RPIPNPTCTFDDAFQCYPEVMENIKKAGFQKPTPIQSQAWPIVLQGIDLIGVAQTGTGKT 293 IP P +F+ AF +M I+K GF+KP+PIQSQ WP++L G D IGV+QTG+GKT Sbjct: 76 VKIPPPVNSFEQAFGSNASIMGEIRKNGFEKPSPIQSQMWPLLLSGQDCIGVSQTGSGKT 135 Query: 294 LCYLMPGFIHLVLQPSL KGQRNRPGMLVLTPTRELALQVEGECCKYSYKGLRΞVC 348 L +L+P +H+ Q + + Q+ P _+LVL+PTRELA Q+EGE KYSY G +ΞVC Sbjct: 136 LAFLLPALLHIDAQLAQYEKNDEEQKPSPFVLVLSPTRELAQQIEGEVKKYSYNGYKSVC 195 Query: 349 VYGGGNRDEQIEELKKGVDIIIATPGRLNDLQMSNFVNLKNITYLVLDEADKMLDMGFEP 408 +YGGG+R EQ+E + GV+I+IATPGRL DL ++L ++TY+VLDEAD+MLDMGFE Sbjct: 196 LYGGGSRPEQVEACRGGVEIVIATPGRLTDLSNDGVISLASVTYVVLDEADRMLDMGFEV 255 Query: 409 QIMKILLDVRPDRQTVMTSATWPHSVHRLAQSYLKEPMIVYVGTLDLVAVSSVKQNIIVT 468 I +IL ++RPDR +TSATWP V +L Y KE ++ G+LDL + SV Q Sbjct: 256 AIRRILFEIRPDRLVALTSATWPEGVRKLTDKYTKEAVMAVNGSLDLTSCKSVTQFFEFV 315 Query: 469 TEEEKW SHMQTFLQSMSSTD-KVIVFVSRKAVADHLSSDLILGNISVESLHGDREQR 524 + ++ + FL + + K+I+FV K +ADHLSSD + 1+ + LHG R Q Sbjct: 316 PHDSRFLRVCEIVNFLTAAHGQNYKMIIFVKSKVMADHLSSDFCMKGINSQGLHGGRSQS 375 Query: 525 DREKALENFKTGKVRILIATDLASRGLDVHDVTHVYNFDFPRNIEEYVHRIGRTGRAGRT 584 DRE +L ++G+V+IL+ATDLASRG+DV D+THV N+DFP +IEEYVHR+GRTGRAGR Sbjct: 376 DREMSLNMLRSGEVQILVATDLASRGIDVPDITHVLNYDFPMDIEEYVHRVGRTGRAGRK 435 Query: 585 GVSITTLTRNDWRVASELINILERANQSIPEELVSMAERFEAHQRKREMERKMERPQGRP 644 G +++ L ND LI ILE++ Q +P++L AE++ K + R RP R Sbjct: 436 GEAMSFLWWNDRSNFEGLIQILEKSEQEVPDQLRRDAEKYRL KCQSGRDGPRPSFRN 492 Query: 645 KK 646 K Sbjct: 493 NK 494 Pedant information for DKFZphtes3_14h21, frame 3 Report for DKFZphtes3_14h21.3 [LENGTH] 648 [MW] 72873.51 [pi] 8.84 [HOMOL] TREMBL :CEY54G11A_9 gene: "Y54G11A.3"; Caenorhabditis elegans cosmid Y54G11A le- 101 [FUNCAT] 04.01.04 rrna processing [S. cerevisiae, YNL112w] 2e-97 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YNL112w] 2e-97 [FUNCAT] 04.05.03 mrna processing (splicing) [S. cerevisiae, YPL119C] 4e-72 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YOR204w] 2e-70 [FUNCAT] 05.04 translation (initiation, elongation and termination) [S. cerevisiae, YOR204w] 2e- 70 [ FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YBR237w] le-61 [FUNCAT] 1 genome replication, transcription, recombination and repair [H. influenzae, HI0892] 2e-49 [FUNCAT] j mrna translation and ribosome biogenesis [H. influenzae, HI0231 RNA] le- 48 [FUNCAT] 04.99 other transcription activities [S. cerevisiae, YDL160C] 9e- -45 [FUNCAT] 04.05.01.07 chromatin modification [S. cerevisiae, YMR290C] 3e- -44 [FUNCAT] 09.01 biogenesis of cell wall [S. cerevisiae, YJL033w] 2e- -36 [FUNCAT] 98 classification not yet clear-cut [S. cerevisiae, YOR046C] 7e- -32 [FUNCAT] 30.16 mitochondrial organization [S. cerevisiae, YDR194C] 2e- -28 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YGL064c] 5e-10 [FUNCAT] 11.10 cell death [S. cerevisiae, YMR190c] 2e-08 [FUNCAT] 03.19 recombination and dna repair [S. cerevisiae, YMR190c] 2e-08 [FUNCAT] r general function prediction [M. jannaschn, MJ1401] le-07 [BLOCKS] BL00039D DEAD-box subfamily ATP-dependent helicases proteins (BLOCKS] BL00039C DEAD-box subfamily ATP-dependent helicases proteins [BLOCKS] BL00039B DEAD-box subfamily ATP-dependent helicases proteins [BLOCKS] BL00039A DEAD-box subfamily ATP-dependent helicases proteins [PIRKW] nucleus 4e-96 [PIRKW] RNA binding 3e-87 [PIRKW] DEAD box 5e-50 [PIRKW] transmembrane protein 4e-27 [PIRKW] DNA binding 3e-67 [PIRKW] recF recombination pathway 3e-10 [PIRKW] ATP 4e-96 [PIRKW] purine nucleotide binding 5e-50 [PIRKW] P-loop 4e-96 [PIRKW] hydrolase 9e-45 [PIRKW] protein biosynthesis 5e-50 [PIRKW] ATP binding le-61 [SUPFAM] WW repeat homology 8e-88 [SUPFAM] DEAD/H box helicase homology 4e-96 [SUPFAM] unassigned DEAD/H box helicases 7e-87 [SUPFAM] ATP-dependent RNA helicase DBP1 4e-96 [SUPFAM] ATP-dependent RNA helicase DHH1 2e-43 [SUPFAM] recQ protein 3e-10 [SUPFAM] Bloom's syndrome helicase 5e-07 [SUPFAM] translation initiation factor eIF-4A 5e-50 [SUPFAM] recQ helicase homology 3e-10 [SUPFAM] tobacco ATP-dependent RNA helicase DB10 8e-88 [PROSITE] DEAD ATP HELICASE 1 [PROSITE] ATP_GTP_A 1 [PFAM] Helicases conserved C-terminal domain [PFAM] KH domain family of RNA binding proteins [PFAM] DEAD and DEAH box helicases [KW] Alpha_Beta [KW] LOW COMPLEXITY 8.49 % SEQ MSHHGGAPKASTWVVASRRSSTVSRAPERRPAEELNRTGPEGYSVGRGGRWRGTSRPPEA SEG xxxxxxxxxxxxxxxxx PRD cccccccccceeeeeecccccccccccccccccccccccccccccccccccccccccccc SEQ VAAGHEELPLCFALKSHFVGAVIGRGGSKIKNIQSTTNTTIQIIQEQPESLVKIFGSKAM SEG xxxxxxxxxxxxxxx PRD cccccccccchhhhhcccceeeecccccccccccccccceeeeecccccceeeeeccchh SEQ QTKAKAVIDNFVKKLEENYNSECGIDTAFQPSVGKDGSTDNNVVAGDRPLIDWDQIREEG SEG PRD hhhhhhhhhhhhhhhhhhhccccccccccccccccccccccccccccccccccccccccc SEQ LKWQKTKWADLPPIKKNFYKESTATSAMSKVEADSWRKENFNITWDDLKDGEKRPIPNPT SEG PRD chhhhhhhcccccccccccccccccchhhhhhhhhhhhhhheeeeecccccccccccccc SEQ CTFDDAFQCYPEVMENIKKAGFQKPTPIQSQAWPIVLQGIDLIGVAQTGTGKTLCYLMPG SEG PRD ccccccccccchhhhhhhhhhcccccccccccccccccccceeeeeecccccceeeecce SEQ FIHLVLQPSLKGQRNRPGMLVLTPTRELALQVEGECCKYSYKGLRSVCVYGGGNRDEQIE SEG PRD eeeeccccccccccccceeeeeccchhhhhhhhhhhhhhhccceeeeeeccccccchhhh SEQ ELKKGVDIIIATPGRLNDLQMSNFVNLKNITYLVLDEADKMLDMGFEPQIMKILLDVRPD SEG PRD hhhhceeeeeeccccchhhhhhhccccccceeeehhhhhhhhhcccchhhhhhhhhhccc SEQ RQTVMTSATWPHSVHRLAQSYLKEPMIVYVGTLDLVAVSSVKQNIIVTTEEEKWSHMQTF SEG PRD ceeeeeecccchhhhhhhhhhhhheeeeeecccccccccccceeehhhhhchhhhhhhhh SEQ LQSMSSTDKVIVFVSRKAVADHLSSDLILGNISVESLHGDREQRDREKALENFKTGKVRI SEG PRD hhhhcccceeeeeeehhhhhhhhhhhhhhcccceeecccccchhhhhhhhhhhhccccee SEQ LIATDLASRGLDVHDVTHVYNFDFPRNIEEYVHRIGRTGRAGRTGVSITTLTRNDWRVAS SEG xxxxxxxxxxxx PRD eeehhhhhhcccccceeeeeeeccccccccceeeecccccccccceeeeeeccccchhhh SEQ ELINILERANQΞIPEELVSMAERFEAHQRKREMERKMERPQGRPKKFH SEG xxxxxxxxxxx PRD hhhhhhhhhccccchhhhhhhhhhhhhhhhhhhhhhhccccccccccc Prosite for DKFZphtes3_14h21.3 PS00017 286->294 ATP_GTP_A PDOC00017 PS00039 394->403 DEAD ATP HELICASE PDOC00039 Pfam for DKFZphtes3_14h21.3 HMM_NAME DEAD and DEAH box helicases HMM *gLpPWILRnIyeMGFEkPTPIQQqAIPιILeGRDVMACAQTGΞGKTAAF P++++NI+++GF KPTPIQ+QA+PI+L+G D+++ AQTG+GKT+++ Query 248 QCYPEVMENIKKAGFQKPTPIQSQAWPIVLQGIDLIGVAQTGTGKTLCY 296 HMM HPMLQHIDwdPWpqpPQd.. PrALILAPTRELAMQIQEEcRkFgkHMng L+P ++H+ +P +++ Q+ P +L+L+PTRELA+Q++ EC K+++ + Query 297 LMPGFIHLVLQP-SLKGQRNRPGMLVLTPTRELALQVEGECCKYSYK-G- 343 HMM IRImcIYGGtnMRdQMRmLeRGpPHIVIATPGRLIDHIERgtldLDrleM +R++C+YGG N ++Q+++L++G+ +I+IATPGRL D+ +++ ++L++I++ Query 344 LRSVCVYGGGNRDEQIEELKKGV-DIIIATPGRLNDLQMSNFVNLKNITY 392 HMM LVMDEADRMLDMGFIDQIRrlMrqIPMpwNRQTMMFSATMPdelqELARr LV+DEAD+MLDMGF++QI++I+ ++ ++RQT+M SAT+P ++ +LA Query 393 LVLDEADKMLDMGFEPQIMKILLDVR—PDRQTVMTSATWPHSVHRLAQS 440 HMM FMRNPIRInld.MdElTtnEnlkQwYiyVerEMWKfdcLcrLIe* ++++P + ++ D +++ +KQ +1+ E++K + ++++ Query 441 YLKEPMIVYVGTLDLVAVS-SVKQNIIVTT-EEEKWSHMQTFLQ 482 HMM_NAME KH domain family of RNA binding proteins HMM *rIιIPedhMGMIIGKGGsNIRqIREEYgvrINIPdecCeDstdRIITIt + + ++++G++IG+GGΞ I++I++ ++++I I++E+ + + + I Query 71 CFALKSHFVGAVIGRGGSKIKNIQΞTTNTTIQIIQEQ-P ESLVKIF 115 HMM G* G Query 116 G 116 HMM_NAME Helicases conserved C-termmal domain HMM *EιleeWLknl....GIrvmYIHGdMpQeERdelMddFNnGEynVLIcTD + +++ L+ + +I+V ++HGD++Q++R++++++F++G+ ++LI+TD Query 497 KAVADHLSSDLILGNISVESLHGDREQRDREKALENFKTGKVRILIATD 545 HMM VggRGIDIPdVNHVINYDMPWNPEqYIQRIGRTgRIG* +++RG+D+ DV HV+N+D+P+N+E Y++RIGRTGR+G Query 546 LASRGLDVHDVTHVYNFDFPRNIEEYVHRIGRTGRAG 582 DKFZphtes3_14pl4 group: testes derived DKFZphtes3_14pl4 encodes a novel 159 amino acid protein without similarity to known proteins. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . unknown complete cDNA, complete eds, few EST hits Sequenced by BMFZ Locus : unknown Insert length: 3969 bp Poly A stretch at pos. 3948, polyadenylation signal at pos. 3927 1 GAAGCCCAGG CTCTCCTTAG TTGACTGTGT GTTAATCACC CAGCAATTTC 51 ATTACTCAAC AGCTCTCCAG AGTTGCACAT TACAGCTGGG GTAGAAATTG 101 GGTGCTGAAG GCCAGGCAGA GCATTTGGCT GTAGGGAGGC CGATCCTCCT 151 CGGGCCTGTT ACCGGCGGGT CTTTGTTCTT AGACCTGGGG TTCTTGGCCT 201 CACGGATTCC AAGGAATGGA ACGTTGGGCC ATGCGTGTGA ACGAGCTCTA 251 TGTCGATGAC CCAGACAAGG ACAGCGGTGG CAAGATCGAC GTCAGTCTGA 301 ACATCAGTTT ACCCAATCTG CACTGCGAGT TGGTTGGGCT TGACATTCAG 351 GATGAGATGG GCAGGCACGA AGTGGGCCAC ATCGACAACT CCATGAAGAT 401 CCCGCTGAAC AATGGGGCAG GCTGCCGCTT CGAGGGGCAG TTCAGCATCA 451 ACAAGGTATG GAAGCCCTGC CTCAGCCCTT TCTACCTGCT CCCCTTTCCT 501 GCTGTCTCCC CGCTCCCTGG AAACTGGTTG TGGAGGCACT CACTCGACCT 551 GACCCTGACA CAGCCCCCAG CAAGCGAGGG TTCGTGTCCA GCTGCCTGGC 601 CGTTCCTGCT GAGAATCTGG ATGGGGGTCC AGGCTCCCTG GGGTTTTAAG 651 CCCCTGATGG CTGGTTCAGG AAGGAGCTAC TCTTCTCTCC AGTGAGGGGG 701 ACAATGATGA GAAGACCTGA GGATTTGCAG CCCCCAGCCC TGGGTTCAAG 751 TCCCAGCTCT ACCCCTTCTT GGCCCCTACA AGTCACTTGA CCCATCTTAG 801 GCTGAGGGTG TGATGGCGAT AATAGTATCA CGATACCACC CACTTCACAA 851 AGTTTGTGTG GGGATTAAAT GAGCTAATGC AGATTCATTC ATTCAGAAAA 901 ATTTTTGAAT GGCACGTTCT GTGTTCCAGG GTCGGTGATA GGCTCTGGGG 951 CAGCGTTCCT GGGCTGGTGG GGCTCCCATT CTGGTAGAGG GAGACAGTCT 1001 ACAAACCAGA AAGCATCAGG GATGCTAAGT GCAGTGATGA GGAATAAAGC 1051 CAAGGGGAGT GAGATGAGGT GGGCTTGAAA GTACCTTGTC CGCTCAGAAG 1101 GACCATTCAA GGTTCACTGT TGTTTTGTCC TCAGAACCAG GAGCTTCAGA 1151 TCCTAAGTCA AGTGGGTGAA CGCAGTGCCC TTGGGAGGGC CGAGGCACCC 1201 GGTGGCAGCT GGCAGGGTTT TGCTCAGCAC GTGCCGGCCT TCCTCGAAGC 1251 TCGGTACTGT CACAGTGGAG CCTCTCAACA ACGCTGTGAG GCAGCACCAT 1301 TTGACAGGTT AGGATGCTGG GGCCCAGAGA GGTTAAGTGT CTTGCCCGAG 1351 GTCACACAGC TATCTGCATG TCCCACAACT CCCCTTCCCA GCCCCAGCCA 1401 AACTGAGCCA CTGGCCACTC CTGGCTTCTC CTTGTCCCTC CTGCAGCCTC 1451 TGCTCAGAAC GCCCTTCCTC CAGACCCTGA CACCTGAGCT GGGGTTGCAA 1501 AGTCACTGGC CACATCCAGC CCAAAGATAA ATTTTGTTTG TCCAGTATAG 1551 CATTTAACTG CATCAGAACC AGTATGAAAA GACCAGGAAT CCAGATTTCT 1601 GGCTTTTAAA AGTCAGAGGC TCTCACTACA CTGGGTCCGT GTTCCCGCTA 1651 TGACAATGAC CTGGCACCAA TGGGCAGTGT TCCCCTTTAG AGAGGGTGTG 1701 TGCTGTCCCT TCCCACAGTC CCTGGCAGGC GGCTGGAAGG CCAGGCCTGG 1751 TCATCTGTCA AGCAGGGTGG ACTTCTTACG TGACAGTTCA GGGCTCCCTT 1801 AAGTGCTAAA GCAGAAGCTG CAAGGCTTTC TTAAGGTTTC GAGTGTTGCT 1851 GGGAGAAATC TGCTGCATGT TGTGGGTTAA AGGGAGTCTC TCACCAGCCC 1901 AGGCCCTCAG. GAGGAGGAGA TACCAGGAGG CAGGGATGCT GGGGGTCGTG 1951 GTTCACTGGG * GGCTCTCTCT GCCCATGAGC TGCCACACAG CACCTTTGCC 2001 ATGCCCCGTA ATTTGGATTT TATGGTGGTT GTGATGGAAA GCCATTTGAG 2051 GGTTTTGAAC AGGGAGGCAA TGTAATCAGA TTTATGCCTT AGAACTGGAC 2101 TATCCAATAG GTTGCCACCA GCCACATAAG GCTATTTAAA TTAATTCAAA 2151 TTAAATGTAC AATTCAGTCA CTCATTCTCA TCAACCACAT TTCAAGTGCT 2201 CAAAGCCACG TGCTGGCTAG GGGCCACAGC GTTAGACAGT GCAGAGAGAA 2251 AGCACTTCCA TCGCTGAGGA AAGTTCTGCT GGACCGCACA CCCTTAGAAG 2301 GATGGCTCTG GTGGCCGGGC GCGGTGGCTC AAACCTGTAA TCCCAGCACT 2351 TTGGGAGGCC GAGGTGGGTG GATCACGAGG TCAGGAGATC GAGACCATCC 2401 CGGCTAACAT GGTGAAACCC TGCCTCTACT AAAAATACAA AAAAAAACAA 2451 AATTAGCCGG GCGTGGTTGC GGGCACCTGT AGTCCCAGCT ACTCAGGAGG 2501 CTGAGGCGGG AGAATGGCAT GAACCCGGGA GGTGGAGCTT GCAGTGAGCC 2551 AAGATCGTAC CACTGCACTC CAGTCTGGGC GACAGAGTGA GACTCCATCT 2601 CAAAACAAAC AAAAAAAGGA TGGGGCTGGG CTGGAGAGGG TGGCAGGCAG 2651 TGGTTGTGGC AGTGGAGCTG GGGAGATGTG GTCGGATTAG GGAGGTAGAA 2701 TCAATAAGAC TCAGTGAAGA ATCGGATGTG GGGGTAAGGG CACATGTGGA 2751 AGCAAAGAAA CCTTTGACGT CTTTGTCTTG ACAACCGGGT GGTCCTGTTT 2801 CTAGACATGG AAGCTTAGAA AAGCCTGGAG TCTGTGGGAA GTAGGTAGGG 2851 CTGGGCACTG GTCATTCCAC TCTGGTTTCC TTTGGGGTTC CCATTAGGTG 2901 TCTACAGGGA GAGGTGAAAT TGGAAGTTGG AGGTGTGGAG AGTTCAGGAG 2951 AGGGTTCTGG ACCACAGATG TTGAGGTGGG AGTCATTAGT GAATAGATGA 3001 TGTTGGAAGT CATGGGTCCT CAGAGTGGGG GCTCCTTAAG CCTCCAGGCC 3051 AGCAGCATCA GCATCACCTG GGAGATTGTT AGGAATGCAG ATTCTCAGGC 3101 CCCCCTAAGA CCCACCGACT CTGTGCTAGA ACAAGCGCCC CTCAGAGATT 3151 CTGATGCCAC TGAAGTTTGA GGAGCATTGG TTTAAGCAAG ATTACCTACG 3201 GAGAGGCTGT AGATCCGTGT TCTAAACCTG GGGTCCACAG ACACCCCCAA 3251 GAAGAGCGGA TTGAATGCAA GAGATCTATG AAGTTGGATG GGGGAAAAAT 3301 TGACATCTTT ATTTTTGCTA AACTCGATCT AAAGTTTAGC ATTTCCATCT 3351 GCGATGAATG TAGGCCACAA ACCACAGTAG TATTAGCAGT GCCTGGGACC 3401 TCCTCAACAA CAGAAATTGC CGGTATTTAT AGCACGTTAC AGTTGTTGCA 3451 GATAATTTCC AGAGACTGTT TATATGCACC ACTGTTTTAA AATTACGGTG 3501 ATTGGCCAGG TGCAGTGGCT CACACCTGTA ATCCCAGCAC TTTGGGAGGC 3551 CAAAGTGGGT GGATCACTTG AGGAGTTCAA GACCAGCCTG GTCAACATGT 3601 CAAAACCCTG TATCTACAAA AAAATACAAA AGTTAACCAA GCCTATGCTT 3651 GTAGTCACAG CTACTCGGGA GGCCGAGGTG GGAGGGTCTT CTGAGCCCAG 3701 GGAGGTAGAG GCTTCAGTGA GCTGAGATCG CACCACCACA CTCCAGCCTG 3751 GGTGACAGAG TGAAACCCTT AATCAATCAG TCAATAAAAA TTACAGTAAT 3801 TATTAGACCC ACCACTAGGT CATCTTATTT GATGCATCAG TAAAGCAGCA 3851 TATTCAAATG TGGATTTTTA AATATTTTAA TTACTATTTA AATATCTCTT 3901 TACTTTGTAA TCCTATGCAT TTTACGCATT AAAACATTTT AAGCATTTAA 3951 AAAAAAAAAA AAAAAAAAA BLAST Results No BLAST result Medlme entries No Medlme entry Peptide information for frame 3 ORF from 216 bp to 692 bp; peptide length: 159 Category: putative protein Classification: no clue 1 MERWAMRVNE LYVDDPDKDS GGKIDVSLNI SLPNLHCELV GLDIQDEMGR 51 HEVGHIDNSM KIPLNNGAGC RFEGQFSINK VWKPCLSPFY LLPFPAVSPL 101 PGNWLWRHSL DLTLTQPPAS EGSCPAAWPF LLRIWMGVQA PWGFKPLMAG 151 ΞGRSYSSLQ BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_14pl4, frame 3 No Alert BLASTP hits found Pedant information for DKFZphtes3_14pl4, frame 3 Report for DKFZphtes3_14pl4.3 [LENGTH] 159 [MW] 17778.55 [pi] 5.74 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YAL042w] 5e-04 [KW] Alpha_Beta SEQ MERWAMRVNELYVDDPDKDSGGKIDVSLNISLPNLHCELVGLDIQDEMGRHEVGHIDNSM PRD ccchhhhhhhhccccccccccceeeeeeccccccccceeeehhhhhhcccceeecccccc SEQ KIPLNNGAGCRFEGQFSINKVWKPCLSPFYLLPFPAVSPLPGNWLWRHSLDLTLTQPPAS PRD eeecccccceeecccccccccccccccccccccccccccccccccccccccccccccccc SEQ EGSCPAAWPFLLRIWMGVQAPWGFKPLMAGSGRSYSSLQ PRD ccccccchhhhhhhhhhhccccccccccccccccccccc (No Prosite data available for DKFZphtes3_14pl4.3) (No Pfam data available for DKFZphtes3_14pl4.3) DKFZphtes3_14p7 group: testes derived DKFZphtes3_14p7 encodes a novel 702 ammo acid protein with very weak similarity to kinesm associated protein KAP3. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes. weak similarity to kinesin associated protein KAP3 complete cDNA, complete eds, few EST hits Sequenced by BMFZ Locus: unknown Insert length: 2497 bp Poly A stretch at pos. 2424, polyadenylation signal at pos. 2400 1 GGAATCCAAA GAAACAGTTA TGATGGGGGA CTCTATGGTG AAAATAAATG 51 GGATTTATTT AACAAAATCA AATGCTATTT GCCACTTAAA GAGTCACCCA 101 CTTCAGCTAA CTGATGATGG AGGCTTCAGT GAAATAAAGG AGCAAGAAAT 151 GTTCAAAGGA ACAACATCTT TACCATCTCA TCTCAAGAAT GGAGGGGACC 201 AGGGGAAGAG ACATGCGAGG GCCTCATCAT GCCCCAGTAG CTCAGACCTG 251 AGCAGGCTGC AAACCAAAGC AGTCCCAAAA GCTGACCTGC AAGAAGAGGA 301 CGCAGAAATA GAAGTAGACG AAGTCTTTTG GAATACAAGG ATTGTACCGA 351 TTTTGCGTGA ATTAGAAAAG GAAGAAAACA TTGAAACGGT TTGTGCTGCT 401 TGCACACAAC TTCATCATGC TTTAGAGGAA GGAAACATGC TTGGAAATAA 451 ATTTAAGGGA AGAAGTATTC TCCTGAAGAC CCTGTGTAAA CTAGTTGATG 501 TTGGTTCAGA CTCGCTCAGC CTTAAACTTG CAAAAATAAT TCTAGCACTT 551 AAAGTGAGTA GAAAGAATCT TCTTAATGTC TGCAAACTTA TATTTAAAAT 601 TAGCAGGAAT GAGAAGAATG ATTCTTTGAT TCAAAATGAC AGCATTCTGG 651 AATCATTATT GGAGGTACTA AGAAGTGAAG ACCTGCAAAC TAACATGGAA 701 GCTTTTTTAT ACTGTATGGG GTCTATAAAG TTCATTTCTG GAAATCTGGG 751 ATTTCTTAAT GAAATGATCA GCAAAGGTGC TGTGGAAATA CTGATAAATT 801 TGATAAAACA AATAAATGAG AACATCAAGA AATGTGGTAC ATTTTTGCCT 851 AATTCGGGCC ACTTGCTAGT CCAGGTGACT GCTACATTGA GAAACTTGGT 901 TGATTCATCA TTAGTAAGAA GTAAGTTCCT AAACATCAGT GCCCTTCCCC 951 AGCTCTGCAC GGCAATGGAA CAGTACAAGG GTGACAAGGA CGTCTGTACC 1001 AATATTGCCA GAATATTCAG CAAACTTACT TCTTACCGTG ACTGCTGCAC 1051 AGCCTTGGCC AGCTATTCCA GATGTTATGC CTTATTTCTG AATCTAATTA 1101 ACAAATACCA GAAGAAGCAG GATTTAGTCG TCCGTGTTGT TTTTATTCTT 1151 GGCAACCTGA CGGCAAAAAA TAACCAGGCT CGTGAACAAT TTTCCAAAGA 1201 GAAAGGGAGC ATCCAAACTC TGCTGTCATT ATTCCAGACG TTCCATCAGC 1251 TGGATCTGCA TTCCCAGAAG CCGGTGGGCC AACGAGGCGA GCAGCACAGG 1301 GCGCAGAGGC CGCCGTCAGA GGCAGAGGAC GTGCTCATCA AGCTGACTCG 1351 TGTGCTGGCC AACATTGCCA TCCACCCGGG CGTGGGCCCG GTGCTGGCCG 1401 CCAACCCGGG GATAGTGGGC CTGCTCCTGA CCACGCTGGA ATACAAGTCA 1451 CTTGATGATT GTGAGGAGCT GGTGATCAAT GCTACAGCGA CAATCAACAA 1501 TTTATCTTAC TACCAAGTGA AGAATTCCAT AATTCAAGAC AAAAAGCTAT 1551 ATATTGCTGA ATTGCTCTTA AAGCTTCTTG TCAGTAACAA CATGGATGGA 1601 ATCCTGGAGG CTGTGCGTGT TTTCGGAAAT CTCTCCCAGG ACCATGATGT 1651 CTGCGATTTC ATTGTGCAGA ACAATGTCCA CAGGTTCATG ATGGCGCTGC 1701 TGGATGCTCA GCATCAGGAT ATCTGCTTTT CTGCCTGTGG TGTTCTCCTC 1751 AATCTCACTG TGGATAAAGA CAAGCGTGTC ATCTTGAAAG AAGGAGGTGG 1801 CATTAAAAAG TTAGTGGACT GTTTAAGAGA TTTGGGTCCT ACTGATTGGC 1851 AGCTGGCCTG CTTGGTTTGT AAAACTTTAT GGAACTTCAG TGAAAACATC 1901 ACTAATGCTT CGTCATGTTT TGGAAATGAA GACACCAACA CACTCTTACT 1951 CTTGCTCTCA TCATTTTTAG ATGAAGAACT AGCACTGGAT GGCAGTTTTG 2001 ATCCAGACCT AAAAAACTAT CACAAACTCC ATTGGGAAAC AGAATTCAAA 2051 CCTGTGGCAC AGCAGCTTCT AAACCGAATT CAGAGACATC ACACCTTCCT 2101 GGAACCCCTG CCCATTCCCT CTTTCTAACA TGATGCAGAT TAACAGTAGA 2151 AACGAGAACT CACGTCTCCC TCATTCTTAA GAACTGGTAA CAAACGTGAA 2201 CATTTTTTTC AGCATTAACA AATGTGGAAA GTTTTTCAAG AACTGGTTTT 2251 AGTGAGTAGC TGAAGTATTT TTTAAAATTA AGCATTTCTT CTTGTTAGGT 2301 ATTATGGAAA AATGAATATA CACATTATAT TTCCTGTTGA GAGAAATGTA 2351 AGATGAAAAT ATGTGCATTT TCAAGTAAAT GACTTTTTCT TCTATTCTCT 2401 ATTAAACAAT TTAGTTCTAG TCTTAAAAAA AAAAAAAAAA AAAAAAAAAA 2451 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAA BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 2 ORF from 20 bp to 2125 bp; peptide length: 702 Category: putative protein 1 MMGDSMVKIN GIYLTKSNAI CHLKSHPLQL TDDGGFSEIK EQEMFKGTTS 51 LPSHLKNGGD QGKRHARASS CPSSSDLSRL QTKAVPKADL QEEDAEIEVD 101 EVFWNTRIVP ILRELEKEEN IETVCAACTQ LHHALEEGNM LGNKFKGRSI 151 LLKTLCKLVD VGSDSLSLKL AKIILALKVS RKNLLNVCKL IFKISRNEKN 201 DSLIQNDΞIL ESLLEVLRSE DLQTNMEAFL YCMGSIKFIS GNLGFLNEMI 251 SKGAVEILIN LIKQINENIK KCGTFLPNSG HLLVQVTATL RNLVDSSLVR 301 SKFLNISALP QLCTAMEQYK GDKDVCTNIA RIFSKLTSYR DCCTALASYS 351 RCYALFLNLI NKYQKKQDLV VRVVFILGNL TAKNNQAREQ FSKEKGSIQT 401 LLSLFQTFHQ LDLHSQKPVG QRGEQHRAQR PPSEAEDVLI KLTRVLANIA 451 IHPGVGPVLA ANPGIVGLLL TTLEYKSLDD CEELVINATA TINNLSYYQV 501 KNSIIQDKKL YIAELLLKLL VSNNMDGILE AVRVFGNLSQ DHDVCDFIVQ 551 NNVHRFMMAL LDAQHQDICF SACGVLLNLT VDKDKRVILK EGGGIKKLVD 601 CLRDLGPTDW QLACLVCKTL WNFSENITNA SSCFGNEDTN TLLLLLSSFL 651 DEELALDGSF DPDLKNYHKL HWETEFKPVA QQLLNRIQRH HTFLEPLPIP 701 SF BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_14p7, frame 2 TREMBL :MMD367_1 product: "KAP3B"; Mus musculus mRNA for KAP3B, complete eds., N = 2, Score = 97, P = 0.00039 >TREMBL:MMD367_1 product: "KAP3B"; Mus musculus mRNA for KAP3B, complete eds. Length = 772 HSPs: Score = 97 (14.6 bits), Expect = 3.9e-04, Sum P(2) = 3.9e-04 Identities = 45/163 (27%), Positives = 77/163 (47%) Query: 442 LTRVLANIAIHPGVGPVLAANPGIVGLLLTTLEYKSLDDCEELVINATATINNLSYYQVK 501 L +++ NI+ H G P VG L + S D+ EE VI T+ NL+ + Sbjct: 483 LMKMIRNISQHDG—PTKNLFIDYVGDLAAQI SSDEEEEFVIECLGTLANLTIPDLD 537 Query: 502 -NSIIQDKKLYIAELLLKLLVSNNMDG-ILEAVRVFGNLSQDHDVCDFIVQNNVHRFMMA 559 ++++ KL + L KL D +LE V + G +Ξ D + ++ + ++ Sbjct: 538 WELVLKEYKL-VPFLKDKLKPGAAEDDLVLEVVIMIGTVSMDDSCAALLAKSGIIPALIE 596 Query: 560 LLDAQHQDICFSACGVLL NLTVDKDKR-VILKEGGGIKKLVDCLRD 604 LL+AQ +D F C ++ + + R VI+KE L+D + D Sbjct: 597 LLNAQQEDDEF-VCQIIYVFYQMVFHQATRDVIIKETQAPAYLIDLMHD 644 Score = 77 (11.6 bits). Expect = 3.9e-04, Sum P(2) = 3.9e-04 Identities = 42/178 (23%), Positives = 82/178 (46%) Query: 169 KLAKIILALKVSRKNLLNVCK-LIFKISRNEKNDSLIQNDSILESLLEVLRSEDLQTNME 227 K K L V ++ LL V L+ ++ + + + ++N +1+ L++ L + N E Sbjct: 263 KTFKKYQGLVVKQEQLLRVALYLLLNLAEDTRTELKMRNKNIVHMLVKALDRD NFE 318 Query: 228 AFLYCMGSIKFISGNLGFLNEMISKGAVEILINLIKQINENIKKCGTFLPNSGHLLVQVT 287 + + +K +S + N+M+ VE L+ +1 +E++ L + + Sbjct: 319 LLILVVSFLKKLSIFMENKNDMVEMDIVEKLVKMIPCEHEDL LNITLR 366 Query: 288 ATLRNLVDSSLVRSKFLNISALPQLCTAM—EQYKGDKDVCT—NIARI—FSKLTSYRD 341 L D+ L R+K + + LP+L + E YK +C +1+ F + +Y D Sbjct: 367 LLLNLSFDTGL-RNKMVQVGLLPKLTALLGNENYK-QIAMCVLYHISMDDRFKSMFAYTD 424 Query: 342 CCTAL 346 C L Sbjct: 425 CIPQL 429 Score = 69 (10.4 bits). Expect = 2.6e+00, Sum P(2) = 9.2e-01 Identities = 35/146 (23%), Positives = 70/146 (47%) Query: 512 IAELLLKLLVSNNMDGILEAVRVFGNLSQDHDVCDFIVQNNVHRFMMALLDAQHQDICFS 571 I +L+K L +N + ++ V LS + + +V+ ++ ++ ++ +H+D+ Sbjct: 304 IVHMLVKALDRDNFELLILVVSFLKKLSIFMENKNDMVEMDIVEKLVKMIPCEHEDLLNI 363 Query: 572 ACGVLLNLTVDKDKRVILKEGGGIKKLVDCLRDLGPTDW-QLACLVCKTLWNFSENITNA 630 +LLNL+ D R + + G + KL L G ++ Q+A +C L++ Ξ + Sbjct: 364 TLRLLLNLSFDTGLRNKMVQVGLLPKLTALL GNENYKQIA—MC-VLYHISMD-DRF 416 Query: 631 SSCFGNEDT-NTLLLLLSSFLDEELALD 657 S F D L+ +L DE + L+ Sbjct: 417 KSMFAYTDCIPQLMKMLFECSDERIDLE 444 Score = 68 (10.2 bits), Expect = 3.2e-03, Sum P(2) = 3.2e-03 Identities = 18/58 (31%), Positives = 30/58 (51%) Query: 190 LIFKISRNEKN-DSLIQNDSILESLLEVLRΞE DLQTNMEAFLYCMGΞIKFIΞG 241 LI +++RN N + L+ N++ L +L VLR + +L TN+ +C S G Sbjct: 155 LILQLARNPDNLEELLLNETALGALARVLREDWKQSVELATNIIYIFFCFSSFSHFHG 212 Score = 65 (9.8 bits), Expect = 6.4e+00, Sum P(2) = l.Oe+00 Identities = 26/122 (21%), Positives = 53/122 (43%) Query: 283 LVQVTATLRNL VDSSLVRSKFLNISALPQLCTAMEQYKGDKDVCTNIARIFSKLTS 338 +++ TL NL +D LV ++ +P L ++ + D+ + I S Sbjct: 521 VIECLGTLANLTIPDLDWELVLKEY KLVPFLKDKLKPGAAEDDLVLEVV-IMIGTVS 576 Query: 339 YRDCCTALAΞYSRCYALFLNLINKYQKKQDLVVRVVFILGNLTAKNNQAREQFSKEKGSI 398 D C AL + Ξ + L+N Q+ + V +++++ + + R+ KE + Sbjct: 577 MDDSCAALLAKSGIIPALIELLNAQQEDDEFVCQIIYVFYQMVF-HQATRDVIIKETQAP 635 Query: 399 QTLLSL 404 L+ L Sbjct: 636 AYLIDL 641 Score = 65 (9.8 bits), Expect = 6.4e+00, Sum P(2) = 1.0e+00 Identities = 44/177 (24%), Positives = 79/177 (44%) Query: 481 CE-ELVINATATIN-NLSYYQ-VKNSIIQDKKLYIAELLLKLLVSNNMDGILEAVRVFGN 537 CE E ++N T + NLS+ ++N ++Q + + L LL + N I A+ V + Sbjct: 355 CEHEDLLNITLRLLLNLSFDTGLRNKMVQ VGLLPKLTALLGNENYKQI—AMCVLYH 409 Query: 538 LSQDHDVCD-FIVQNNVHRFMMALLDAQHQDICFSACGVLLNLTVDKDKRVILKEGGGIK 596 +S D F + + + M L + + 1 +NL +K ++ EG G+K Sbjct: 410 ISMDDRFKSMFAYTDCIPQLMKMLFECSDERIDLELISFCINLAANKRNVQLICEGNGLK 469 Query: 597 KLVDCLRDLGPTDWQLACLVCKTLWNFSENITNASSCFGNEDTNTLLLLLSSFLDEELAL 656 L+ R L D L+ K + N S++ + F + L +SS +EE + Sbjct: 470 MLMK—RALKLKD PLLMKMIRNISQHDGPTKNLF-IDYVGDLAAQISSDEEEEFVI 522 Query: 657 D 657 + Sbjct: 523 E 523 Score = 61 (9.2 bits), Expect = 1.6e-02, Sum P(2) = 1.6e-02 Identities = 20/66 (30%), Positives = 34/66 (51%) Query: 304 LNISALPQLCTAM-EQYKGDKDVCTNIARIFSKLTSYRDCCTALASYSRCYALFLNLINK 362 LN +AL L + E +K ++ TNI IF +S+ + Y + AL +N+I+ Sbjct: 171 LNETALGALARVLREDWKQSVELATNIIYIFFCFSSFSHFHGLITHY-KIGALCMNIIDH 229 Query: 363 YQKKQDL 369 K+ +L Sbjct: 230 ELKRHEL 2 36 Pedant information for DKFZphtes3_14p7, frame 2 Report for DKFZphtes3_14p7.2 [LENGTH] 708 [MW] 79266.35 [pi] 6.57 [FUNCAT] 30.25 vacuolar and lysosomal organization [S. cerevisiae, YEL013w] 3e-04 [FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YEL013w] 3e-04 [FUNCAT] 09.25 vacuolar and lysosomal biogenesis [S. cerevisiae, YEL013w] 3e-04 [BLOCKS] BL00923F Aspartate and glutamate racemases proteins [BLOCKS] BL00288B Tissue inhibitors of metalloproteinases proteins [PROSITE] MYRISTYL 9 [PROSITE] AMIDATION 1 [PROSITE] CK2_PHOSPHO_SITE 12 [PROSITE] PKC_PHOSPHO_SITE 7 [PROSITE] ASN_GLYCOSYLATION 11 [KW] Alpha_Beta [KW] LOW COMPLEXITY 7.49 % SEQ ESKETVMMGDSMVKINGIYLTKΞNAICHLKSHPLQLTDDGGFSEIKEQEMFKGTTSLPSH SEG PRD cccceeeecccceeeccccccccceeeeecccccccccccccchhhhhhhhccccccccc SEQ LKNGGDQGKRHARASSCPΞSSDLSRLQTKAVPKADLQEEDAEIEVDEVFWNTRIVPILRE SEG xxxxxxxxxx PRD cccccccchhhhhhcccccccchhhhhhhccccchhhhhhhhhhhcccccceeehhhhhh SEQ LEKEENIETVCAACTQLHHALEEGNMLGNKFKGRSILLKTLCKLVDVGSDSLSLKLAKII SEG xxxxxxxxxx PRD hhhhhcchhhhhhhhhhhhhhhhcccccccccccccchhhhhheeeeccccchhhhhhhh SEQ LALKVSRKNLLNVCKLIFKISRNEKNDSLIQNDSILESLLEVLRSEDLQTNMEAFLYCMG SEG xxxx PRD hhhhhhhhhhhhhhhhhccccccccccccccccchhhhhhhhhhhccchhhhhhhhhhcc SEQ SIKFISGNLGFLNEMISKGAVEILINLIKQINENIKKCGTFLPNSGHLLVQVTATLRNLV SEG PRD ceeeeccccchhhhhhhcchhhhhhhhhhhhhcccccccccccccceeeeeehhhhhhhh SEQ DSSLVRSKFLNISALPQLCTAMEQYKGDKDVCTNIARIFSKLTSYRDCCTALASYSRCYA SEG PRD ccchhhhheeeeccchhhhhhhhhhccccceeeehhhhhhhhhhcccchhhhhhhhhhhh SEQ LFLNLINKYQKKQDLVVRVVFILGNLTAKNNQAREQFSKEKGSIQTLLSLFQTFHQLDLH SEG PRD hhhhhhhhhhhhhhhheeeeeeeccccccchhhhhhhhhhhchhhhhhhhhhhhhhhhcc SEQ SQKPVGQRGEQHRAQRPPSEAEDVLIKLTRVLANIAIHPGVGPVLAANPGIVGLLLTTLE SEG PRD ccccccccccccccccccccchhhhhhhhhhhhhhhccccccceeeccccchhhhhhhhh SEQ YKSLDDCEELVINATATINNLSYYQVKNSIIQDKKLYIAELLLKLLVSNNMDGILEAVRV SEG xxxxxxxxxxxxx PRD hhccccchhhhhhhhheeeecccccccceeeehhhhhhhhhhhhhhhccccchhhhhhhh SEQ FGNLSQDHDVCDFIVQNNVHRFMMALLDAQHQDICFSACGVLLNLTVDKDKRVILKEGGG SEG PRD cccccccccceeeeeecchhhhhhhhhhhhcccceeeecceeeeeeecccceeeeecccc SEQ IKKLVDCLRDLGPTDWQLACLVCKTLWNFSENITNASSCFGNEDTNTLLLLLSSFLDEEL SEG xxxxxxxxxxxxx PRD hhhhhhhhhccccccccchhhhhhhhccccccccccccccccccccceeeehhhhhhhhh SEQ ALDGSFDPDLKNYHKLHWETEFKPVAQQLLNRIQRHHTFLEPLPIPSF SEG xxx PRD hhccccccccchhhhhhhhhhchhhhhhhhhhhhhhhheeeecccccc Prosite for DKFZphtes3_14p7.2 PS00001 206- ->210 ASN GLYCOSYLATION PDOC00001 PS00001 212- ->216 ASN GLYCOSYLATION PDOC00001 PΞ00001 311- ->315 ASN GLYCOSYLATION PDOC00001 PS00001 385- ->389 ASN GLYCOSYLATION PDOC00001 PS00001 493- ->497 ASN GLYCOSYLATION PDOC00001 PS00001 500- ->504 ASN GLYCOSYLATION PDOC00001 PS00001 543- ->547 ASN GLYCOSYLATION PDOC00001 PS00001 584- ->588 ASN GLYCOSYLATION PDOC00001 PS00001 628- ->632 ASN GLYCOSYLATION PDOC00001 PS00001 632- ->636 ASN GLYCOSYLATION PDOC00001 PS00001 635- ->639 ASN GLYCOSYLATION PDOC00001 PS00005 173- ->176 PKC PHOSPHO SITE PDOC00005 PS00005 186- ->189 PKC PHOSPHO SITE PDOC00005 PS00005 241- ->244 PKC PHOSPHO SITE PDOC00005 o o ca H U α. o o o o o o o o o υ α o α o Q o o o o o ω ω ω ω ω ω ω ω ω ω ω ω ω ω OT OT OT OT OT OT OT OT OT OT OT OT OT I I I I I I I I I l l o o o o o O O o o o 0 o o o x OT OT OT OT OT OT OT OT OT OT OT O O O O O O O O υ I l l l l I I I 1 I I υ υ ΓM x, x υ. o υ υ υ υ o υ o o ιθιθ OO DKFZphtes3 15al3 group: testes derived DKFZphtes3_15al3 encodes a novel 387 amino acid protein with weak similarity to S. cerevisiae Hopl. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . similarity to S. cerevisiae Hopl complete cDNA, complete eds, potential start codon at Bp 116, 3 EST hits S. cerevisiae Hoplp is a meiosis-specific protein Sequenced by GBF Locus: unknown Insert length: 1848 bp Poly A stretch at pos. 1766, no polyadenylation signal found 1 GGAAAGCGCA TGCGCGTCGG GCACAGCGCG TGCAGCCTCG TGCAGCTCTT 51 CTGGTCTCCG GCGCCCGCCC CTCAGACGTA ATGTTGAATT AAAGAAAATA 101 CTTTATCAGA AGAAGATGGC CACTGCCCAG TTGCAGAGGA CTCCCATGAG 151 TGCACTGGTA TTTCCCAATA AGATATCAAC TGAACACCAG TCTTTGGTGT 201 TAGTGAAGAG GCTTCTAGCA GTTTCAGTAT CCTGTATCAC GTATTTGAGG 251 GGAATATTCC CAGAATGCGC TTATGGAACA AGATATCTAG ATGATCTTTG 301 TGTCAAAATA CTGAGAGAAG ATAAAAATTG CCCAGGATCT ACACAGTTAG 351 TGAAATGGAT GCTAGGATGT TATGATGCTT TACAGAAAAA ATATGTATAC 401 ACAAACCCAG AAGATCCTCA GACAATTTCA GAATGTTACC AATTCAAATT 451 CAAATACACC AATAATGGAC CACTCATGGA CTTCATAAGT AAAAACCAAA 501 GCAACGAATC TAGCATGTTG TCTACTGACA CCAAGAAAGC AAGCATTCTC 551 CTCATTCGCA AGATTTATAT CCTAATGCAA AATCTGGGGC CTTTACCTAA 601 TGATGTTTGT TTGACCATGA AACTTTTTTA CTATGATGAA GTTACACCCC 651 CAGATTACCA GCCTCCCGGT TTTAAGGATG GTGATTGTGA AGGAGTTATA 701 TTTGAAGGGG AACCTATGTA TTTAAATGTG GGAGAAGTCT CAACACCTTT 751 TCACATCTTC AAAGTAAAAG TGACCACTGA GAGAGAACGA ATGGAAAATA 801 TTGACTCAAC TATACTATCA CCAAAACAAA TAAAAACACC ATTTCAAAAA 851 ATCCTGAGGG ACAAAGATGT AGAAGATGAA CAGGAGCATT ATACAAGTGA 901 TGATTTGGAC ATTGAAACTA AAATGGAAGA ACAGGAAAAA AACCCTGCAT 951 CTTCTGAACT TGAAGAACCA AGTTTAGTTT GTGAGGAAGA TGAAATTATG 1001 AGGTCTAAAG AAAGTCCAGA TCTTTCTATT TCTCATTCTC AGGTTGAGCA 1051 GTTAGTCAAT AAAACATCTG AACTTGATAT GTCTGAAAGC AAAACAAGAA 1101 GTGGAAAAGT CTTTCAGAAT AAAATGGCAA ATGGAAATCA ACCAGTAAAA 1151 TCTTCCAAAG AAAATCGGAA GAGAAGTCAA CATGAATCTG GGAGAATAGT 1201 CCTCCATCAC TTTGATTCTT CTAGTCAAGA GTCAGTGCCA AAAAGGAGAA 1251 AGTTTAGTGA ACCAAAGGAA CATATATAAA AATTATTTTT GTTCTGCAGG 1301 CTTGCAGAGT TCTTCTCACC ATTTAAACTG AAGGACCCTA TATTATATTT 1351 CCCTAACTCT GAAGATGTAT ATGTAGTTTA AAGCAGTTTG TACACTAAAA 1401 CTAAGTTTTT GGCTGACTGT CATATTGTGG TCCTTAATCT TGAGATAAAT 1451 CCAATAGAAC TTTTGAATAA AAGCAAAAGT ACAAATGTCA TAATTGATTC 1501 GGTAATAAGT AAAATTTCAA AATTGATTTT GTTCATTACC TACTTAATAT 1551 TTCCTTTAAA TATATACTAA CTGTTAAGGC CCTCTAATGC CATTTTTCTA 1601 AACAGTAATG TTTACTTTGG TATTAAAATT TGGTATGGAT TCACTTTTTA 1651 CTTATGTTAA AATTATACCA TTTAACTGGC TCTTTTGTCA TTGTGCTGTT 1701 ATTAAAACAA TGTTCTTCAA TATTTTGACA TAATGTATTA ACATTTTAAT 1751 ATATAATGTA CAATTTAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAGG 1801 GGCGGCCGCT CTAGAGGATC CAAGCTTACG TACAAAAAAA AAAAAAGG BLAST Results o BLAST result Medline entries o Medlme entry Peptide information for frame 2 ORF from 116 bp to 1276 bp; peptide length: 387 Category: similarity to known protein 1 MATAQLQRTP MSALVFPNKI STEHQSLVLV KRLLAVSVSC ITYLRGIFPE 51 CAYGTRYLDD LCVKILREDK NCPGSTQLVK WMLGCYDALQ KKYVYTNPED 101 PQTISECYQF KFKYTNNGPL MDFISKNQSN ESSMLSTDTK KASILLIRKI 151 YILMQNLGPL PNDVCLTMKL FYYDEVTPPD YQPPGFKDGD CEGVIFEGEP 201 MYLNVGEVST PFHIFKVKVT TERERMENID STILSPKQIK TPFQKILRDK 251 DVEDEQEHYT SDDLDIETKM EEQEKNPASS ELEEPSLVCE EDEIMRSKES 301 PDLSISHSQV EQLVNKTSEL DMSESKTRSG KVFQNKMANG NQPVKSSKEN 351 RKRSQHESGR IVLHHFDSSS QESVPKRRKF SEPKEHI BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_15al3, frame 2 TREMBL :ATAC2130_3 product: "F1N21.3"; The sequence of BAC F1N21 from Arabidopsis thaliana chromosome 1, complete sequence., N = 1, Score = 274, P = 5.7e-22 TREMBL :SC9877_9 gene: "hopl"; S. cerevisiae chromosome IX cosmid 9877., N = 2, Score = 126, P = 7. le-09 PIR:A34691 meiosis-specific protein HOP1 - yeast (Saccharomyces cerevisiae), N = 2, Score = 126, P = 7.8e-08 >TREMBL:ATAC2130_3 product: "F1N21.3"; The sequence of BAC F1N21 from Arabidopsis thaliana chromosome 1, complete sequence. Length = 562 HSPs: Score = 274 (41.1 b ts), Expect = 5.7e-22, P = 5.7e-22 Identities = 84/290 (28%), Positives = 145/290 (50%) Query: 22 TEHQSLVLVKRLLAVSVSCITYLRGIFPECAYGTRYLDDLCVKILREDKNCPGSTQLVKW 81 TE SL+L + LL +++ I+Y+RG+FPE + + + L +KI + S +L+ W Sbjct: 11 TEQDSLLLTRNLLRIAIFNISYIRGLFPEKYFNDKSVPALDMKIKKLMPMDAESRRLIDW 70 Query: 82 M-LGCYDALQKKYVYT NPEDPQTISECYQFKFKYTNNGP--LMDFIΞK—NQSN 130 M G YDALQ+KY+ T D I E Y F F Y+++ +M I++ N+ N Sbjct: 71 MEKGVYDALQRKYLKTLMFSICETVDGPMIEE-YSFSFSYSDSDSQDVMMNINRTGNKKN 129 Query: 131 ESSMLST DTKKASILLIRKIYILMQNLGPLPNDVCLTMKLFYYDEVTPPDYQPP 184 ST + ++ ++R + LM+ L +P++ + MKL YYD+VTPPDY+PP Sbjct: 130 GGIFNSTADITPNQMRSSACKMVRTLVQLMRTLDKMPDERTIVMKLLYYDDVTPPDYEPP 189 Query: 185 GFKD—GDCEGVIFEGEPMYLNVGEVSTPFHIFKVKVTT ERERMENIDSTILS 235 F+ D ++ P+ + +G V++ + +KV + E + M++ D + Sbjct: 190 FFRGCTEDEAQYVWTKNPLRMEIGNVNSKHLVLTLKVKSVLDPCEDENDDMQD-DGKSIG 248 Query: 236 PKQIKTPFQKILRDKDVEDEQEHY TSDDLDIETKMEEQEKNPAΞSE 281 P + Q D ++ QE+ DD D E ++ ++PA +E Sbjct: 249 PDSVHDD-QPSDSDSEISQTQENQFIVAPVEKQDDDDGEVDEDDNTQDPAENE 300 Pedant information for DKFZphtes3_15al3, frame 2 Report for DKFZphtes3_15al3.2 [LENGTH] 387 [MW] 44417.64 [pi] 5.57 [HOMOL] TREMBL :ATAC2130_3 product: "F1N21.3"; The sequence of BAC F1N21 from Arabidopsis thaliana chromosome 1, complete sequence. 9e-23 [FUNCAT] 09.13 biogenesis of chromosome structure [S. cerevisiae, YIL072w] 7e-ll [FUNCAT] 03.19 recombination and dna repair [S. cerevisiae, YIL072w] 7e-ll [FUNCAT] 03.13 meiosis [S. cerevisiae, YIL072w] 7e-ll [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YIL072w] 7e-ll [PIRKW] nucleus 2e-09 [ PIRKW] zmc finger 2e-09 [PIRKW] DNA binding 2e-09 [PROSITE] MYRISTYL 1 [PROSITE] CAMP_PHOSPHO_SITE 3 [PROSITE] CK2_PHOSPHO_SITE 12 [PROSITE] PKC_PHOSPHO_SITE 7 [PROSITE] ASN_GLYCOSYLATION 3 [KW] Alpha_Beta SEQ MATAQLQRTPMSALVFPNKISTEHQSLVLVKRLLAVSVSCITYLRGIFPECAYGTRYLDD PRD cccccccccccccccccccchhhhhhhhhhhhhhhhhhhhheeeeecccccccccccchh SEQ LCVKILREDKNCPGSTQLVKWMLGCYDALQKKYVYTNPEDPQTISECYQFKFKYTNNGPL PRD hhhhhhhccccccccccccccccchhhhhhhhhhhcccccccchhhhhheeeeeccccce SEQ MDFISKNQSNESSMLSTDTKKASILLIRKIYILMQNLGPLPNDVCLTMKLFYYDEVTPPD PRD eeeecccccccceeecccchhhhhhhhhhhhhhhhhcccccccccceeeeeeeeeccccc SEQ YQPPGFKDGDCEGVIFEGEPMYLNVGEVΞTPFHIFKVKVTTERERMENIDSTILSPKQIK PRD cccccccccccceeeeeccceeeeeccccccceeeeeecccchhhhhcccccccccchhh SEQ TPFQKILRDKDVEDEQEHYTSDDLDIETKMEEQEKNPASSELEEPSLVCEEDEIMRΞKES PRD hhhhhhhhhhhhhhhhhhhcccchhhhhhhhhhhcccccccccccccchhhhhhhhhhcc SEQ PDLSISHSQVEQLVNKTSELDMSESKTRSGKVFQNKMANGNQPVKSSKENRKRSQHESGR PRD ccccccchhhhhhhhhhcccccccccccccceeeeeccccccccchhhhhhhhhhcccce SEQ IVLHHFDSSSQESVPKRRKFSEPKEHI PRD eeeeecccccccccccccccccccccc Prosite for DKFZphtes3_15al3.2 PS00001 127->131 ASN_GLYCOSYLATION PDOC00001 PS00001 130->134 ASN_GLYCOSYLATION PDOC00001 PS00001 315->319 ASN_GLYCOSYLATION PDOC00001 PS00004 140-M44 CAMP_PHOSPHO_SITE PDOC00004 PS00004 351->355 CAMP_PHOSPHO_SITE PDOC00004 PS00004 378->382 CAMP_PHOSPHO_SITE PDOC00004 PS00005 139-M42 PKC_PHOSPHO_SITE PDOC00005 PS00005 167->170 PKC_PHOSPHO_SITE PDOC00005 PS00005 221->224 PKC_PHOSPHO_SITE PDOC00005 PS00005 235->238 PKC_PHOSPHO_SITE PDOC00005 PS00005 329->332 PKC_PHOSPHO_SITE PDOC00005 PS00005 346->349 PKC_PHOSPHO_SITE PDOC00005 PS00005 358->361 PKC_PHOSPHO_SITE PDOC00005 PS00006 96->100 CK2_PHOSPHO_SITE PDOC00006 PS00006 103->107 CK2_PHOSPHO_SITE PDOC00006 PS00006 177->181 CK2_PHOSPHO_SITE PDOC00006 PS00006 221->225 CK2_PHOSPHO_SITE PDOC00006 PS00006 260->264 CK2_PHOSPHO_SITE PDOC00006 PS00006 268->272 CK2_PHOSPHO_SITE PDOC00006 PS00006 280->284 CK2_PHOSPHO_SITE PDOC00006 PS00006 308->312 CK2_PHOSPHO_SITE PDOC00006 PS00006 318->322 CK2_PHOSPHO_SITE PDOC00006 PΞ00006 346->350 CK2_PHOSPHO_SITE PDOC00006 PS00006 354->358 CK2_PHOSPHO_SITE PDOC00006 PS00006 369->373 CK2_PHOSPHO_SITE PDOC00006 PS00008 84->90 MYRISTYL PDOC00008 (No Pfam data available for DKFZphtes3 15al3.2) DKFZphtes3_15c24 group: metabolism DKFZphtes3_15c24 encodes a novel 404 amino acid protein with strong similarity to 2- hydroxyacid dehydrogenases . The novel protein contains a D-isomer specific 2-hydroxyacιd dehydrogenases signature. Proteins with such a signature have similar enzymatic activities: D-lactate dehydrogenase (EC 1.1.1.28), catalyzes the reduction of D-lactate to pyruvate. D-glycerate dehydrogenase (EC 1.1.1.29) catalyzes the reduction of hydroxypyruvate to glycerate. 3-phosphoglycerate dehydrogenase (EC 1.1.1.95), catalyzes the oxidation of D-3-phosphoglycerate to 3-phosphohydroxypyruvate . Therefore the novel protein is a new 2-hydroxyacιd dehydrogenase. The new protein can find application in modulation of 2-hydroxyacιd dehydrogenases-dependent pathways and as a new enzyme for biotechnologic production processes. strong similarity to C. elegans T03F1.1 potential start at Bp 55 matches kozak consensus PyCCatgG Sequenced by GBF Locus : unknown Insert length: 1956 bp Poly A stretch at pos. 1929, polyadenylation signal at pos. 1903 1 CGAAGGCGGC GGCGAAGGCC CGGGCTGGGA GCGTTGGCGG CCGGAGTCCC 51 AGCCATGGCG GAGTCTGTGG AGCGCCTGCA GCAGCGGGTC CAGGAGCTGG 101 AGCGGGAACT TGCCCAGGAG AGGAGTCTGC AGGTCCCGAG GAGCGGCGAC 151 GGAGGGGGCG GCCGGGTCCG CATCGAGAAG ATGAGCTCAG AGGTGGTGGA 201 TTCGAATCCC TACAGCCGCT TGATGGCATT GAAACGAATG GGAATTGTAA 251 GCGACTATGA GAAAATCCGT ACCTTTGCCG TAGCAATAGT AGGTGTTGGT 301 GGAGTAGGTA GTGTGACTGC TGAAATGCTG ACAAGATGTG GCATTGGTAA 351 GTTGCTACTC TTTGATTATG ACAAGGTGGA ACTAGCCAAT ATGAATAGAC 401 TTTTCTTCCA ACCTCATCAA GCAGGATTAA GTAAAGTTCA AGCAGCAGAA 451 CATACTCTGA GGAACATTAA TCCTGATGTT CTTTTTGAAG TACACAACTA 501 TAATATAACC ACAGTGGAAA ACTTTCAACA TTTCATGGAT AGAATAAGTA 551 ATGGTGGGTT AGAAGAAGGA AAACCTGTTG ATCTAGTTCT TAGCTGTGTG 601 GACAATTTTG AAGCTCGAAT GACAATAAAT ACAGCTTGTA ATGAACTTGG 651 ACAAACATGG ATGGAATCTG GGGTCAGTGA AAATGCAGTT TCAGGGCATA 701 TACAGCTTAT AATTCCTGGA GAATCTGCTT GTTTTGCGTG TGCTCCACCA 751 CTTGTAGTTG CTGCAAATAT TGATGAAAAG ACTCTGAAAC GAGAAGGTGT 801 TTGTGCAGCC AGTCTTCCTA CCACTATGGG TGTGGTTGCT GGGATCTTAG 851 TACAAAACGT GTTAAAGTTT CTGTTAAATT TTGGTACTGT TAGTTTTTAC 901 CTTGGATACA ATGCAATGCA GGATTTTTTT CCTACTATGT CCATGAAGCC 951 AAATCCTCAG TGTGATGACA GAAATTGCAG GAAGCAGCAG GAGGAATATA 1001 AGAAAAAGGT AGCAGCACTG CCTAAACAAG AGGTTATACA AGAAGAGGAA 1051 GAGATAATCC ATGAAGATAA TGAATGGGGT ATTGAGCTGG TATCTGAGGT 1101 TTCAGAAGAG GAACTGAAAA ATTTTTCAGG TCCAGTTCCA GACTTACCTG 1151 AAGGAATTAC AGTGGCATAC ACAATTCCAA AAAAGCAAGA AGATTCTGTC 1201 ACTGAGTTAA CAGTGGAAGA TTCTGGTGAA AGCTTGGAAG ACCTCATGGC 1251 CAAAATGAAG AATATGTAGA TAATGGACTG GGATATATTG TATTTCTCAT 1301 GTTAAAGCCT CTTCCCTTGA AATTAAAAAA AAATTTTAAC TGATAAAACT 1351 TAGGGCAACA TTAATTAATG TATATTCTTA CCTGAATTGT TATACTTTTT 1401 GAAAATCCTG TGACTTGCCT GTTTCTCCCC GCTCCAACGA AATCATTAAC 1451 TCTCCTAAAA TGTGTTTCAT TCTAGTAAGA AAACCTCAAA GGATATTGTA 1501 GGATATAAAT CTTACTTGAA AACATAGCTG TTGAAATGTT TTGGCCTTTT 1551 GGAGTGGGGG AAGGACAAAT CTGATCCTGT AATCTTTTTC TTTCCAGTAA 1601 TCCCTTGTGT CTGTTGCATG AGGACATGGA CAATAAAGTA GTATATGATC 1651 CTCAGATACA GGGAGAAGGA CAAGGCATAC AGCTTATTGA TTAGAGCTGG 1701 CAAGCATCTG CTCATTATGT TTGGAATTGC TTTCTATAAG AAAATTGCCC 1751 ACTACTACTA ACTTGATCAA CAATGAATTC AAAATAGTTA ACCTATGAAA 1801 TAACATCCTC TCAAATGTTT GCTGATGAAG TACAAGTTGA AATGTAGTTA 1851 TTGGAAAAGT CTGTAACCTG TGGATCATAT ATATTCAAAG TGAGACAAAG 1901 GCAAATAAAA AGCAGCTATT TTCATGAATA GACAAAAAAA AAAAAAAAAA 1951 AAAAAG BLAST Results o BLAST result Medline entries No Medline entry Peptide information for frame 1 ORF from 55 bp to 1266 bp; peptide length: 404 Category: similarity to unknown protein Classification: Metabolism Prosite motifs: D 2 HYDROXYACID DH 1 (76-105) 1 MAESVERLQQ RVQELERELA QERSLQVPRS GDGGGGRVRI EKMSSEVVDS 51 NPYSRLMALK RMGIVSDYEK IRTFAVAIVG VGGVGSVTAE MLTRCGIGKL 101 LLFDYDKVEL ANMNRLFFQP HQAGLSKVQA AEHTLRNINP DVLFEVHNYN 151 ITTVENFQHF MDRISNGGLE EGKPVDLVLS CVDNFEARMT INTACNELGQ 201 TWMESGVSEN AVSGHIQLII PGESACFACA PPLVVAANID EKTLKREGVC 251 AASLPTTMGV VAGILVQNVL KFLLNFGTVS FYLGYNAMQD FFPTMSMKPN 301 PQCDDRNCRK QQEEYKKKVA ALPKQEVIQE EEEIIHEDNE WGIELVSEVΞ 351 EEELKNFSGP VPDLPEGITV AYTIPKKQED SVTELTVEDS GESLEDLMAK 401 MKNM BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_15c24, frame 1 TREMBL :CEUT03F1_11 gene: "T03F1.1"; Caenorhabditis elegans cosmid T03F1., N = 1, Score = 1204, P = 1.9e-122 TREMBL :ATAC98_3 gene: "YUP8H12.3"; Arabidopsis thaliana chromosome 1 YAC yUP8H12 complete sequence., N = 1, Score = 733, P = 1.5e-72 PIR:A69319 thiamine biosynthesis protein (thiF) homolog - Archaeoglobus fulgidus, N = 1, Score = 218, P = 1.8e-17 TREMBL :AF022796_4 gene: "moeB"; product: "MoeB"; Staphylococcus carnosus molybdenum cofactor biosynthetic gene cluster, complete sequence., N = 1, Score = 220, P = 3.7e-16 >TREMBL:CEUT03F1_11 gene: "T03F1.1"; Caenorhabditis elegans cosmid T03F1. Length = 419 HSPs: Score = 1204 (180.6 bits), Expect = 1.9e-122, P = 1.9e-122 Identities = 241/367 (65%), Positives = 293/367 (79%) Query: 37 RVRIEKMSSEVVDSNPYSRLMALKRMGIVSDYEKIRTFAVAIVGVGGVGSVTAEMLTRCG 96 R +IEK+S+EVVDSNPYSRLMAL+RMGIV++YE+IR VA+VGVGGVGSV AEMLTRCG Sbjct: 48 RQKIEKLSAEVVDSNPYSRLMALQRMGIVNEYERIREKTVAVVGVGGVGSVVAEMLTRCG 107 Query: 97 IGKLLLFDYDKVELANMNRLFFQPHQAGLSKVQAAEHTLRNINPDVLFEVHNYNITTVEN 156 IGKL+LFDYDKVE+ANMNRLF+QP+QAGLSKV+AA TL ++NPDV EVHN+NITT++N Sbjct: 108 IGKLILFDYDKVEIANMNRLFYQPNQAGLSKVEAARDTLIHVNPDVQIEVHNFNITTMDN 167 Query: 157 FQHFMDRISNGGLEEGKPVDLVLSCVDNFEARMTINTACNELGQTWMESGVSENAVSGHI 216 F F++RI G L +GK +DLVLSCVDNFEARM +N ACNE Q WMESGVSENAVSGHI Sbjct: 168 FDTFVNRIRKGSLTDGK-IDLVLSCVDNFEARMAVNMACNEENQIWMESGVSENAVSGHI 226 Query: 217 QLIIPGESACFACAPPLVVAANIDEKTLKREGVCAASLPTTMGVVAGILVQNVLKFLLNF 276 Q I PG++ACFAC PPLVVA+ IDE+TLKR+GVCAASLPTTM WAG LV N LK+LLNF Sbjct: 227 QYIEPGKTACFACVPPLVVASGIDERTLKRDGVCAASLPTTMAVVAGFLVMNTLKYLLNF 286 Query: 277 GTVSFYLGYNAMQDFFPTMSMKPNPQCDDRNCRKQQEEYKKKVAALPKQ-EV-IQEEEEI 334 G VS Y+GYNA+ DFFP S+KPNP CDD +C ++Q+EY++KVA P EV + EEE + Sbjct: 287 GEVSQYVGYNALSDFFPRDSIKPNPYCDDSHCLQRQKEYEEKVANQPVDLEVEVPEEETV 346 Query: 335 IHEDNEWGIELVSEVSEEELKNFSGPVPDLPEGITVAYTIPKKQEDSVTELTVEDSGESL 394 +HEDNEWGIELV+E SE + S + G+ AY P K+ D+ TEL+ + + Sbjct: 347 VHEDNEWGIELVNE-SEPSAEQSSSL—NAGTGLKFAYE-PIKR-DAQTELSPAQA—AT 399 Query: 395 EDLMAKMKN 403 D M +K+ Sbjct: 400 HDFMKSIKD 408 Pedant information for DKFZphtes3_15c24, frame 1 Report for DKFZphtes3_15c24.1 [LENGTH] 404 [MW] 44863.36 [pi] 4.79 [HOMOL] TREMBL:CEUT03F1_11 gene: "T03F1.1"; Caenorhabditis elegans cosmid T03F1. le-115 [FUNCAT] h cofactor metabolism [H. influenzae, HI1449] 2e-08 [FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylation, farnesylation and processing) [S. cerevisiae, YDR390c UBA2 - El-like] 4e-07 [FUNCAT] 04.05.05 mrna processing (5 '-end, 3' -end processing and mrna degradation) [S. cerevisiae YDR390C UBA2 - El-like] 4e-07 [FUNCAT] 06.13.01 cytoplasmic degradation [S. cerevisiae, YDR390c UBA2 - El-like] 4e-07 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YDR390c UBA2 - El-like] 4e-07 [FUNCAT] 11.01 stress response [S. cerevisiae, YKL210w UBA1 - El-like] 2e-06 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YKL210w UBA1 - El-like] 2e-06 [BLOCKS] BL01042A Homoserme dehydrogenase proteins [PIRKW] thiamme pyrophosphate le-07 [PIRKW] molybdenum 5e-07 [PIRKW] molybdopterin biosynthesis 5e-07 [SUPFAM] molybdopteπn biosynthesis protein moeB 2e-12 [PROSITE] D_2_HYDROXYACID_DH_l 1 [KW] TRANSMEMBRANE 1 [KW] LOW COMPLEXITY 8.66 % SEQ MAESVERLQQRVQELERELAQERSLQVPRSGDGGGGRVRIEKMSSEVVDSNPYSRLMALK SEG PRD ccchhhhhhhhhhhhhhhhhhhhhhhcccccccccccceeeccccccccccchhhhhhhc MEM SEQ RMGIVSDYEKIRTFAVAIVGVGGVGSVTAEMLTRCGIGKLLLFDYDKVELANMNRLFFQP SEG xxxxxxxxx PRD cccccchhhhhhhheeeeecccccchhhhhhhhhhcccceeeecccccchhhhhhhhhhc MEM MMMMMMMMMMMMMMMMMMMMMM SEQ HQAGLSKVQAAEHTLRNINPDVLFEVHNYNITTVENFQHFMDRISNGGLEEGKPVDLVLS SEG PRD ccccchhhhhhhhhhhhccccceeeeeccccccchhhhhhhhhhhcccccccccceeeee MEM SEQ CVDNFEARMTINTACNELGQTWMESGVSENAVSGHIQLIIPGESACFACAPPLVVAANID SEG PRD cccchhhhhhhhhhhhhhccccccccccccccccceeeeccccccceeeccccccccccc MEM SEQ EKTLKREGVCAASLPTTMGVVAGILVQNVLKFLLNFGTVSFYLGYNAMQDFFPTMSMKPN SEG PRD ccccccccccccccccchhhhhhhhhhhhhhhhhcccceeeccccccccccccccccccc MEM SEQ PQCDDRNCRKQQEEYKKKVAALPKQEVIQEEEEIIHEDNEWGIELVSEVSEEELKNFSGP SEG xxxxxxxxxxxxxxx...xxxxxxxxxxx PRD ccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccceeeeeehhhhhhhhhcccc MEM SEQ VPDLPEGITVAYTIPKKQEDSVTELTVEDSGESLEDLMAKMKNM SEG PRD ccccccceeeeeeehhhhhhhheeeeeccccchhhhhhhhhccc MEM Prosite for DKFZphtes3_15c24.1 PS00065 76->105 D_2_HYDROXYACID_DH_l PDOC00063 (No Pfam data available for DKFZphtes3_15c24.1) DKFZphtes3_15c6 group: transmembrane protein DKFZphtes3_15c6 encodes a novel 118 amino acid protein without similarity to known proteins. The novel protein contains 1 transmembrane region. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes and as a new marker for testicular cells. unknown complete cDNA, complete eds, EST hits Sequenced by GBF Locus : unknown Insert length: 1283 bp Poly A stretch at pos. 1264, no polyadenylation signal found 1 GAGACACTGA GCCCCGAGAC AGTGAGTGGT GGCCTCACTG CTCTGCCCGG 51 CACCCTGTCA CCTCCACTTT GCCTTGTTGG AAGTGACCCA GCCCCCTCCC 101 CTTCCATTCT CCCACCTGTT CCCCAGGACT CACCCCAGCC CCTGCCTGCC 151 CCTGAGGAAG AAGAGGCACT CACCACTGAG GACTTTGAGT TGCTGGATCA 201 GGGGGAGCTG GAGCAGCTGA ATGCAGAGCT GGGCTTGGAG CCAGAGACAC 251 CGCCAAAACC CCCTGATGCT CCACCCCTGG GGCCCGACAT CCATTCTCTG 301 GTACAGTCAG ACCAAGAAGC TCAGGCCGTG GCAGAGCCAT GAGCCAGCCG 351 TTGAGGAAGG AGCTGCAGGC ACAGTAGGGC TTCCTGGCTA GGAGTGTTGC 401 TGTTTCCTCC TTTGCCTACC ACTCTGGGGT GGGGCAGTGT GTGGGGAAGC 451 TGGCTGTCGG ATGGTAGCTA TTCCACCCTC TGCCTGCCTG CCTGCCTGCT 501 GTCCTGGGCA TGGTGCAGTA CCTGTGCCTA GGATTGGTTT TAAATTTGTA 551 AATAATTTTC CATTTGGGTT AGTGGATGTG AACAGGGCTA GGGAAGTCCT 601 TCCCACAGCC TGCGCTTGCC TCCCTGCCTC ATCTCTATTC TCATTCCACT 651 ATGCCCCAAG CCCTGGTGGT CTGGCCCTTT CTTTTTCCTC CTATCCTCAG 701 GGACCTGTGC TGCTCTGCCC TCATGTCCCA CTTGGTTGTT TAGTTGAGGC 751 ACTTTATAAT TTTTCTCTTG TCTTGTGTTC CTTTCTGCTT TATTTCCCTG 801 CTGTGTCCTG TCCTTAGCAG CTCAACCCCA TCCTTTGCCA GCTCCTCCTA 851 TCCCGTGGGC ACTGGCCAAG CTTTAGGGAG GCTCCTGGTC TGGGAAGTAA 901 AGAGTAAACC TGGGGCAGTG GGTCAGGCCA GTAGTTACAC TCTTAGGTCA 951 CTGTAGTCTG TGTAACCTTC ACTGCATCCT TGCCCCATTC AGCCCGGCCT 1001 TTCATGATGC AGGAGAGCAG GGATCCCGCA GTACATGGCG CCAGCACTGG 1051 AGTTGGTGAG CATGTGCTCT CTCTTGAGAT TAGGAGCTTC CTTACTGCTC 1101 CTCTGGGTGA TCCAAGTGTA GTGGGACCCC CTACTAGGGT CAGGAAGTGG 1151 ACACTAACAT CTGTGCAGGT GTTGACTTGA AAAATAAAGT GTTGATTGGC 1201 TAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAGGGCGGCC GCTCTAGAGG 1251 ATCCAAGCTT ACGTAAAAAA AAAAAAAAAA AAG BLAST Results NO BLAST result Medline entries No Medline entry Peptide information for frame 2 ORF from 461 bp to 814 bp; peptide length: 118 Category: putative protein 1 MVAIPPSACL PACCPGHGAV PVPRIGFKFV NNFPFGLVDV NRAREVLPTA 51 CACLPASSLF SFHYAPSPGG LALSFSSYPQ GPVLLCPHVP LGCLVEALYN 101 FSLVLCSFLL YFPAVSCP BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_15c6, frame 2 PIR:S54250 ribosomal protein L2 - Arabidopsis thaliana, N = 1, Score = 76, P = 0.33 >PIR:S54250 ribosomal protein L2 - Arabidopsis thaliana Length = 258 HSPs: Score = 76 (11.4 bits), Expect = 4.0e-01, P = 3.3e-01 Identities = 30/91 (32%), Positives = 4/91 (48%) Query: 15 PGHGAVPVPRIGFKFVNNFPFGLVDVNRAREVLPTACACLPASSLFSFHYAPSPGGLALS 74 PG GA P+ R+ F+ PF + +E+ A C P ΞSL+ A G L Sbjct: 52 PGRGA-PLARVTFRH PFRF KKQKELFVAAEVCTPVSSLYCGKKATLVVGNVLP 103 Query: 75 FSSYPQGPVLLCP HV-PLGCLVEALYNFSLVL 105 S P+G V+ C HV G L A ++++V+ Sbjct: 104 LRSIPEGAVV-CNVEHHVGDRGVLARASGDYAIVI 137 Pedant information for DKFZphtes3_15c6, frame 2 Report for DKFZphtes3_15c6.2 [LENGTH] 118 [MW] 12413.79 [pi] 7.53 [PROSITE] LEUCINE_ZIPPER 1 [PROSITE] MYRISTYL 1 [PROSITE] ASN_GLYCOSYLATION [KW] TRANSMEMBRANE 1 SEQ MVAIPPSACLPACCPGHGAVPVPRIGFKFVNNFPFGLVDVNRAREVLPTACACLPAΞSLF PRD cccccccccccccccccccccccccceeeecccccceeehhhhhhccccceeeccccccc MEM SEQ SFHYAPSPGGLALSFSSYPQGPVLLCPHVPLGCLVEALYNFSLVLCSFLLYFPAVSCP PRD eeecccccccceeeeecccccccccccccccchhhhhhhcchhhhhhhhccccccccc MEM MMMMMMMMMMMMMMMMM. Prosite for DKFZphtes3_15c6.2 PS00001 100->104 ASN_GLYCOSYLATION PDOC00001 PS00008 70->76 MYRISTYL PDOC00008 PS00029 84->106 LEUCINE ZIPPER PDOC00029 (No Pfam data available for DKFZphtes3_15c6.2) DKFZphtes3_15gl4 group: testes derived DKFZphtes3_15gl4 encodes a novel 701 amino acid protein with weak similarity to S. cerevisiae hypothetical protein YOR243c. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes. similarity to YOR243c complete cDNA, complete eds, potential start codon at Bp 35, EST hits Sequenced by GBF Locus : unknown Insert length: 3495 bp Poly A stretch at pos. 3462, no polyadenylation signal found 1 GCCTTCCACT GAACCGAGGC ACTGTTATAG AAGAATGGAA GAAGATACAG 51 ATTATAGAAT CAGGTTTAGT TCTTTGTGTT TCTTTAATGA TCACGTTGGA 101 TTTCATGGCA CTATAAAAAG CTCACCAAGT GACTTTATTG TTATTGAAAT 151 TGATGAACAG GGACAGTTAG TTAATAAGAC CATCGATGAG CCTATTTTCA 201 AGATTAGTGA AATACAACTT GAGCCAAATA ATTTTCCCAA AAAACCAAAA 251 CTAGATCTTC AAAATCTGTC CTTAGAAGAT GGAAGAAACC AAGAAGTTCA 301 TACTTTGATT AAGTACACTG ATGGTGACCA AAATCATCAG TCTGGTTCAG 351 AAAAGGAAGA TACTATCGTT GATGGAACTT CCAAATGTGA AGAAAAAGCT 401 GATGTTTTAA GCTCCTTTTT GGATGAAAAA ACTCATGAGT TACTGAATAA 451 TTTTGCCTGT GATGTAAGAG AGAAGTGGCT TTCTAAAACA GAGCTAATTG 501 GACTACCTCC TGAATTCTCA ATAGGCAGAA TCCTTGACAA AAACCAGAGG 551 GCTAGTTTAC ACAGTGCCAT TAGGCAGAAA TTTCCATTTT TAGTAACTGT 601 AGGAAAAAAC AGTGAAATTG TTGTAAAACC AAATCTTGAA TATAAAGAAC 651 TTTGTCATTT GGTATCTGAA GAGGAAGCAT TTGACTTTTT TAAATATTTG 701 GATGCAAAGA AAGAAAATTC CAAATTTACC TTTAAACCTG ATACAAACAA 751 AGACCACAGA AAAGCTGTCC ACCATTTTGT CAACAAAAAG TTTGGAAACC 801 TTGTGGAAAC CAAATCTTTT TCTAAAATGA ATTGCAGTGC TGGTAATCCG 851 AATGTGGTGG TAACAGTAAG ATTTCGGGAA AAAGCACACA AACGTGGGAA 901 AAGGCCTCTT TCTGAATGCC AAGAAGGAAA AGTTATATAT ACAGCTTTTA 951 CCCTACGAAA GGAAAACCTG GAAATGTTTG AAGCGATTGG TTTTTTAGCT 1001 ATCAAACTTG GTGTTATTCC TTCGGATTTT AGTTATGCAG GCCTTAAAGA 1051 CAAGAAAGCC ATCACCTATC AAGCAATGGT TGTTAGAAAA GTGACTCCAG 1101 AGAGGTTGAA AAATATTGAA AAAGAAATTG AAAAGAAAAG AATGAATGTC 1151 TTTAATATTC GGTCTGTAGA TGATTCCCTG AGACTTGGTC AGCTCAAAGG 1201 AAATCACTTT GATATTGTCA TTAGAAATTT AAAAAAACAA ATAAATGATT 1251 CTGCAAACCT GAGGGAGAGA ATTATGGAAG CAATAGAAAA TGTTAAGAAA 1301 AAAGGCTTTG TGAATTACTA TGGACCACAG AGATTTGGGA AGGGAAGGAA 1351 AGTTCACACA GACCAAATTG GACTAGCTTT GCTGAAGAAT GAAATGATGA 1401 AAGCCATAAA ATTGTTTCTT ACACCAGAAG ACTTGGATGA TCCTGTAAAT 1451 AGAGCAAAGA AGTATTTTCT TCAAACTGAG GATGCTAAAG GCACACTTTC 1501 ATTGATGCCT GAATTCAAAG TGCGTGAGAG AGCATTGTTG GAGGCATTGC 1551 ACCGCTTTGG CATGACCGAG GAAGGTTGTA TCCAGGCATG GTTCTCTTTA 1601 CCCCATTCCA TGCGCATATT CTATGTTCAC GCATATACCA GCAAAATTTG 1651 GAATGAGGCA GTATCTTACA GACTTGAAAC CTATGGAGCA AGAGTAGTGC 1701 AGGGTGATTT GGTCTGTTTG GATGAAGACA TTGATGACGA GAATTTCCCA 1751 AATAGTAAAA TTCACCTGGT AACTGAAGAG GAGGGATCAG CTAATATGTA 1801 TGCAATACAT CAGGTGGTTC TTCCAGTACT TGGATACAAT ATTCAGTACC 1851 CGAAGAACAA AGTAGGGCAG TGGTACCATG ACATACTTAG CAGAGATGGA 1901 CTACAGACAT GTAGGTTTAA AGTACCTACT CTGAAACTGA ATATACCAGG 1951 TTGCTATAGA CAGATTTTGA AACATCCCTG TAATCTCTCA TACCAACTAA 2001 TGGAAGATCA TGACATTGAT GTCAAAACGA AAGGTTCCCA CATTGATGAA 2051 ACAGCTTTGT CTCTTTTGAT CTCTTTTGAT CTTGATGCTT CATGCTATGC 2101 TACCGTTTGT CTGAAGGAAA TAATGAAGCA TGACGTTTAA AACTGATACC 2151 CTTGGTATAA CCATATATAT GTCACCCTTT CCTGTTTTTG AAATTATTGA 2201 TCAGAACAAT ATACAAGGGA AATGCCATAC CTCTGTTTGT GATAGATACC 2251 CCAGAGTAGT TATTACCTCT TTGTGAGATA AGTAATCTTT GATGAAGATT 2301 GAAATACAAT TTCTCATCCA ATTTTTATAT CTTGGCATAC GCTGACCCTC 2351 TTGACCATTT GTAATTTTTT CATATTATCT AAAACAGGTG TTAGAGTCAG 2401 ACAGATTCAT TCTTAGATTC TAGCTCTGAC ACTTACTAGT GATTTTGAGT 2451 ATGTTGTTGA TTTTTTTGTG TGTGGTTACT GATAGAATCA AGACAATTAC 2501 AACTTCATAA ATGACAAATA ATAGGATTAT CTCCACATTT TCTGTTGCTG 2551 GAGGAACAAA ACATTGTGCC CATTTGAAAA TTTTAATTTT TGTTGGTTTA 2601 ACTATCCCAC ATTATAAATC ATCCTTCACC ATTTTATATC AGTTAAATAT 2651 GGGTGTGTTG GGGAGGAATG ACTGGCATGT AGACATGTAT TGATTTAGGA 2701 AGATCTGAGC ATTTCTTTCA TTGTTGGTAA GATATAATGA TGAAATTTAA 2751 AAAGCAGTAT GGAGCATTAT ATATCAGTAA TGTGATATAT ATACTTAAGC 2801 CAGTTTAACC ATTTTGGGAA ATGTTAGCAT TAGGAAATAA AATCCAAAAG 2851 AAGGAAGAGA AGCTATATGC AATGCAAAAT TTGCTTATTG CAATATTTTC 2901 ATATACAGAC ACTAAAAACA GTTTTCAAAG TCCAGCATTA CGTAACTAAA 2951 GTAAGTAAAA TGATGTGTAT CAACTTGATG GTAAAATATG TAGTTATTTA 3001 AAAAAGCAAT GAACAATTTA GTTTCATGAG AAAATGTTGC CCCCTAAAAG 3051 TAGAACACAT ATGTTACAAC TGCAATAATA CTCTGAATTC ATCTTTCACA 3101 AATAAGAGAC ATGTTAGCAT AGTGATTAAA AGCACAGATA TTGGAGACAA 3151 ACTAACCCAG TTTGAACCCT GGCACTGCCA CGTATAGCAC TGCAGCCTTG 3201 GGAAAGTTAT TTAAACTCAT GGGCTTCAGT TTCAACATCT GTAAAATGGG 3251 CATGTTAACA TTGCCTACCT CATAGGATTA CTGTGAGAAT TTTCTAAGTT 3301 AATATATGTA AAGCAACTTT AAAAAGTGCC TGGCACTTAG TTATTGTTAA 3351 GTAAGTGTCT GCAGATGCAA GTTTGGAAGA GAAAAGCAAA TAAATGAAAA 3401 TCCCTTCCTG TTAAGATGAA AAAAAAAAAA AAAAAAAAAA AAAAAAGGGG 3451 CGGCCGCTCA AGATGAAAAA AAAAAAAAAA AAAAAAAAAA AAAGG BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 2 ORF from 35 bp to 2137 bp; peptide length: 701 Category: similarity to unknown protein 1 MEEDTDYRIR FSSLCFFNDH VGFHGTIKSS PSDFIVIEID EQGQLVNKTI 51 DEPIFKISEI QLEPNNFPKK PKLDLQNLSL EDGRNQEVHT LIKYTDGDQN 101 HQSGSEKEDT IVDGTSKCEE KADVLSSFLD EKTHELLNNF ACDVREKWLS 151 KTELIGLPPE FSIGRILDKN QRASLHSAIR QKFPFLVTVG KNSEIVVKPN 201 LEYKELCHLV SEEEAFDFFK YLDAKKENSK FTFKPDTNKD HRKAVHHFVN 251 KKFGNLVETK SFSKMNCSAG NPNVVVTVRF REKAHKRGKR PLSECQEGKV 301 IYTAFTLRKE NLEMFEAIGF LAIKLGVIPS DFSYAGLKDK KAITYQAMVV 351 RKVTPERLKN IEKEIEKKRM NVFNIRSVDD SLRLGQLKGN HFDIVIRNLK 401 KQINDSANLR ERIMEAIENV KKKGFVNYYG PQRFGKGRKV HTDQIGLALL 451 KNEMMKAIKL FLTPEDLDDP VNRAKKYFLQ TEDAKGTLSL MPEFKVRERA 501 LLEALHRFGM TEEGCIQAWF SLPHSMRIFY VHAYTSKIWN EAVSYRLETY 551 GARVVQGDLV CLDEDIDDEN FPNSKIHLVT EEEGSANMYA IHQVVLPVLG 601 YNIQYPKNKV GQWYHDILSR DGLQTCRFKV PTLKLNIPGC YRQILKHPCN 651 LSYQLMEDHD IDVKTKGSHI DETALΞLLIS FDLDASCYAT VCLKEIMKHD 701 V BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_15gl4, frame 2 TREMBL :SPBC1A45P_10 gene: "SPBC1A4.09"; product: "hypothetical protein"; S. pombe chromosome II cosmid clA4 left hand region 1-26184 bp Originates from chimeric cosmid., N = 3, Score = 511, P = 2.9e-57 PIR:S67136 hypothetical protein YOR243c - yeast (Saccharomyces cerevisiae), N = 2, Score = 516, P = 7.3e-54 SWISSPROT :YQ4B_CAEEL HYPOTHETICAL 64.6 KD PROTEIN B0024.ll IN CHROMOSOME V., N = 2, Score = 386, P = 2.1e-34 >PIR:S67136 hypothetical protein YOR243c - yeast (Saccharomyces cerevisiae) Length = 676 HSPs: Score = 516 (77.4 bits), Expect = 7.3e-54, Sum P(2) = 7.3e-54 Identities = 151/498 (30%), Positives = 245/498 (49%) Query: 191 KNSEIVVKPNLEYKELCHLVSEEEAFDFFK-YLDAKKENSKFTFKPDTNKDHRKAVHHFV 249 + E V P L +L + EE+ Y A K + F+ +K R +H + Sbjct: 109 RRQEFNVDPELR-NQLVEIFGEEDVLKIESVYRTANKMETAKNFE DKSVRTKIHQLL 164 Query: 250 NKKFGNLVETKSFSKMNCSAGNPNVVVTVRFREKAHK-RGKRPLSECQEG-KVIYTAFTL 307 + F N +E+ + N +EK ++ R + G + FTL Sbjct: 165 REAFKNELESVTTDTNTFKIARSNRNSRTNKQEKINQTRDANGVENWGYGPSKDFIHFTL 224 Query: 308 RKENLEMFEAIGFLAIKLGVIPSD-FSYAGLKDKKAITYQAMVVRKVTPERLKNIEKEIE 366 KEN + EA+ + KL +PS YAG KD++A+T Q + + K+ +RL + + + Sbjct: 225 HKENKDTMEAVNVIT-KLLRVPSRVIRYAGTKDRRAVTCQRVSISKIGLDRLNALNRTL- 282 Query: 367 KKRMNVFNIRSVDDSLRLGQLKGNHFDIVIRNLKKQINDSANLRERIMEAIENVKKKGFV 426 K M + N D SL LG LKGN F +VIR++ N +L E + +++ + GF+ Sbjct: 283 -KGMIIGNYNFSDASLNLGDLKGNEFVVVIRDVTTG-NSEVSLEEIVSNGCKSLSENGFI 340 Query: 427 NYYGPQRFGKGRKVHTDQIGLALLKNEMMKAIKLFLTPEDLDDPVNR-AKKYFLQTEDAK 485 NY+G QRFG + T IG LL + KA +L L+ +D P ++ A+K + +T+DA Sbjct: 341 NYFGMQRFGTF-SISTHTIGRELLLSNWKKAAELILSDQDNVLPKSKEARKIWAETKDAA 399 Query: 486 GTLSLMPEFKVRERALLEALHRFGMTEEGCIQ—AWFS LPHSMRIFYVHAYTSKIW 539 L MP + E ALL +L E+G A+++ +P ++R YVHAY S +W Sbjct: 400 LALKQMPRQCLAENALLYSLSNQRKEEDGTYSENAYYTAIMKIPRNLRTMYVHAYQSYVW 459 Query: 540 NEAVSYRLETYGARVVQGDLVC LDEDIDDENFPNS KIHLVTEEEGS 585 N S R+E +G ++V GDLV L IDDE+F + VT+E+ Sbjct: 460 NSIASKRIELHGLKLVVGDLVIDTSEKSPLISGIDDEDFDEDVREAQFIRAKAVTQEDID 519 Query: 586 ANMYAIHQVVLPVLGYNIQYPKNK-VGQWYHDILSRDGLQTCRFKVPTLKLNIPGCYRQI 644 + Y + VVLP G+++ YP N+ + Q Y DIL D + + ++ G YR + Sbjct: 520 SVKYTMEDVVLPSPGFDVLYPSNEELKQLYVDILKADNMDPFNMRRKVRDFSLAGΞYRTV 579 Query: 645 LKHPCNLSYQLMEDHDIDVKTKGSHID 671 ++ P +L Y+++ D + + +D Sbjct: 580 IQKPKSLEYRIIHYDDPSQQLVNTDLD 606 Score = 86 (12.9 bits), Expect = 3.2e-01, Sum P(2) = 2.8e-01 Identities = 40/160 (25%), Positives = 77/160 (48%) Query: 22 GFHGTIKSSPSDFIVIEIDEQGQLVNKTIDEPIFKISEIQLEPNNFPKKPKLDLQNLSLE 81 GF G IK +DF+V EID++G++++ T D+ FK+ + +P K +++ + S E Sbjct: 55 GFRGQIKQRYTDFLVNEIDQEGKVIHLT-DKG-FKMPK KPQR—SKEEVNAEKES-E 106 Query: 82 DGRNQEVHTLIKYTDGDQNHQSGS—EKEDTI-VDGTSKCEEKADVLSSFLDEKTHELLN 138 R QE + D + +Q +ED + ++ + K + +F D+ ++ Sbjct: 107 AARRQEFNV DPELRNQLVEIFGEEDVLKIESVYRTANKMETAKNFEDKSVRTKIH 161 Query: 139 NFACDVREKWLSKTELIGLPPE-FSIGRILDKNQRASLHSAIRQ 181 +RE + ++ E + F I R ++N R + I Q Sbjct: 162 QL LREAFKNELESVTTDTNTFKIARS-NRNSRTNKQEKINQ 201 Score = 58 (8.7 bits), Expect = 7.3e-54, Sum P(2) = 7.3e-54 Identities = 10/23 (43%), Positives = 17/23 (73%) Query: 676 SLLISFDLDASCYATVCLKEIMK 698 ++++ F L S YAT+ L+E+MK Sbjct: 638 AVVLKFQLGTSAYATMALRELMK 660 Pedant information for DKFZphtes3_15gl4, frame 2 Report for DKFZphtes3_15gl4.2 [LENGTH] 701 [MW] 80700.96 [pi] 7.31 [HOMOL] PIR:S67136 hypothetical protein YOR243c - yeast (Saccharomyces cerevisiae) 2e- 51 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YOR243c] 8e-53 [BLOCKS] BL01268C [BLOCKS] BL01268B [BLOCKS] BL01268A [SUPFAM] hypothetical protein HI0701 3e-06 [PROSITE] MYRISTYL 7 [PROSITE] AMIDATION 2 [PROSITE] CAMP_PHOSPHO_SITE 1 [PROSITE] CK2_PHOSPHO_SITE 16 [PROSITE] TYR_PHOSPHO_SITE 1 [PROSITE] PKC_PHOSPHO_SITE 13 [PROSITE] ASN_GLYCOSYLATION 5 [KW] Alpha_Beta SEQ MEEDTDYRIRFSSLCFFNDHVGFHGTIKSSPSDFIVIEIDEQGQLVNKTIDEPIFKISEI PRD ccccceeeeeecceeecccccccceeeeecccceeeeeecccceeeeeccccceeeeeee SEQ QLEPNNFPKKPKLDLQNLSLEDGRNQEVHTLIKYTDGDQNHQSGSEKEDTIVDGTSKCEE PRD cccccccccccccccccccccccccccccceeeeccccccccccccceeeeeecccccch SEQ KADVLSSFLDEKTHELLNNFACDVREKWLSKTELIGLPPEFSIGRILDKNQRASLHSAIR PRD hhhhhhhhhhhhhhhhhhhcchhhhhhhhhhheeecccccceeeeeeecchhhhhhhhhh SEQ QKFPFLVTVGKNSEIVVKPNLEYKELCHLVSEEEAFDFFKYLDAKKENSKFTFKPDTNKD PRD hhccceeeecccceeeecccchhhhhhhhhhhhhhhhhhhhhhcccccceeeecccccch SEQ HRKAVHHFVNKKFGNLVETKSFSKMNCSAGNPNVVVTVRFREKAHKRGKRPLSECQEGKV PRD hhhhhhhhhhhhhhheeeeecccceeeecccccceeeechhhhhhhhcccccccccccce SEQ IYTAFTLRKENLEMFEAIGFLAIKLGVIPSDFSYAGLKDKKAITYQAMVVRKVTPERLKN PRD eeeeeeeeccccchhhhhhhhhhhhcccccceeeccccchhhhhhhheeeccccchhhhh SEQ IEKEIEKKRMNVFNIRSVDDSLRLGQLKGNHFDIVIRNLKKQINDSANLRERIMEAIENV PRD hhhhhhhhhheeeeeeccccccccccccccceeeeeehhhhhccccchhhhhhhhhhhhh SEQ KKKGFVNYYGPQRFGKGRKVHTDQIGLALLKNEMMKAIKLFLTPEDLDDPVNRAKKYFLQ PRD hhcccccccccccccccccccchhhhhhhhhhhhhhhhhhhhcccccccchhhhhhhhhh SEQ TEDAKGTLSLMPEFKVRERALLEALHRFGMTEEGCIQAWFSLPHSMRIFYVHAYTSKIWN PRD hcccchhhhhhhhhhhhhhhhhhhhhhcccccchhhhhhhhcccchhhhhhhhhhhhhhh SEQ EAVSYRLETYGARVVQGDLVCLDEDIDDENFPNSKIHLVTEEEGSANMYAIHQVVLPVLG PRD hhhhhhhhhhcceeeccceeeeccccccccccccccceeecccccccccccceeeccccc SEQ YNIQYPKNKVGQWYHDILSRDGLQTCRFKVPTLKLNIPGCYRQILKHPCNLSYQLMEDHD PRD cccccccccchhhhhhhhhhccccccccccccccccccchhhhhhhhccchhhhhhhhcc SEQ IDVKTKGSHIDETALSLLISFDLDASCYATVCLKEIMKHDV PRD ceeeccccchhhhhhheeeeeecccccchhhhhhhhhhccc Prosite for DKFZphtes3_15gl4.2 PS00001 47->51 ASN_GLYCOSYLATION PDOC00001 PS00001 77->81 ASN_GLYCOSYLATION PDOC00001 PS00001 266->270 AΞN_GLYCOSYLATION PDOC00001 PS00001 404->408 AΞN_GLYCOSYLATION PDOC00001 PS00001 650->654 ASN_GLYCOSYLATION PDOC00001 PS00004 351->355 CAMP_PHOSPHO_SITE PDOC00004 PS00005 26->29 PKC_PHOSPHO_SI E PDOC00005 PS00005 105->108 PKC_PHOSPHO_SITE PDOC00005 PS00005 115->118 PKC_PHOSPHO_SITE PDOC00005 PS00005 232->235 PKC_PHOSPHO_SITE PDOC00005 PS00005 237->240 PKC_PHOSPHO_SITE PDOC00005 PS00005 277->280 PKC_PHOSPHO_SITE PDOC00005 PS00005 306->309 PKC_PHOSPHO_SITE PDOC00005 PS00005 381->384 PKC_PHOSPHO_SITE PDOC00005 PS00005 525->528 PKC_PHOSPHO_SITE PDOC00005 PS00005 535->538 PKC_PHOSPHO_SITE PDOC00005 PS00005 544->547 PKC_PHOSPHO_SITE PDOC00005 PS00005 625->628 PKC_PHOSPHO_ΞITE PDOC00005 PS00005 632->635 PKC_PHOSPHO_SITE PDOC00005 PS00006 30->34 CK2_PHOSPHO_SITE PDOC00006 PS00006 49->53 CK2_PHOSPHO_SITE PDOC00006 PS00006 79->83 CK2_PHOSPHO_SITE PDOC00006 PS00006 95->99 CK2_PHOSPHO_SITE PDOC00006 PS00006 103->107 CK2_PHOSPHO_SITE PDOC00006 PS00006 105->109 CK2_PHOSPHO_SITE PDOC00006 PS00006 110->114 CK2_PHOSPHO_SITE PDOC00006 PS00006 116->120 CK2_PHOSPHO_SITE PDOC00006 PS00006 127->131 CK2_PHOSPHO_SITE PDOC00006 PS00006 150->154 CK2_PHOSPHO_SITE PDOC00006 PS00006 211->215 CK2_PHOSPHO_SITE PDOC00006 PS00006 237->241 CK2_PHOSPHO_SITE PDOC00006 PΞ00006 377->381 CK2_PHOSPHO_SITE PDOC00006 PS00006 463->467 CK2_PHOSPHO_SITE PDOC00006 PS00006 580->584 CK2_PHOSPHO_SITE PDOC00006 PS00006 668->672 CK2_PHOSPHO_SITE PDOC00006 PS00007 537->546 TYR_PHOSPHO_SITE PDOC00007 PS00008 25->31 MYRISTYL PDOC00008 PS00008 43->49 MYRISTYL PDOC00008 PS00008 114->120 MYRISTYL PDOC00008 PS00008 326->332 MYRISTYL PDOC00008 PS00008 385->391 MYRISTYL PDOC00008 PS00008 514->520 MYRISTYL PDOC00008 PΞ00008 622->628 MYRISTYL PDOC00008 PS00009 287->291 AMIDATION PDOC00009 PS00009 436->440 AMIDATION PDOC00009 (No Pfam data available for DKFZphtes3_15gl4.2) DKFZphtes3_15hl group: testes derived DKFZphtes3_15hl encodes a novel 672 amino acid protein with very weak similarity to several proteins . No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes. similarity to Hsp70/Hsp90 organizing protein complete cDNA, complete eds, no EST hits Sequenced by GBF Locus : unknown Insert length: 2277 bp Poly A stretch at pos. 2252, polyadenylation signal at pos. 2226 1 AAACCAGATA GAGGTTCTCC AGCTTTTCTT TGATTGTCTC TGCTTTAGCG 51 TCTCTAAATC CGGTCACCAT GTCGGACCCC GAAGGCGAGA CCTTGCGAAG 101 CACCTTTCCC TCTTATATGG CCGAAGGCGA GCGGCTCTAC CTGTGCGGGG 151 AATTTTCTAA AGCCGCGCAG AGCTTCAGCA ACGCTCTTTA CCTTCAGGAT 201 GGAGACAAGA ACTGCCTGGT TGCTCGCTCA AAGTGCTTCC TGAAGATGGG 251 AGACTTGGAG AGATCCCTGA AGGATGCTGA GGCTTCGCTC CAGAGTGACC 301 CAGCTTTCTG TAAGGGGATT TTGCAAAAGG CTGAGACACT GTACACCATG 351 GGAGACTTTG AGTTTGCCTT GGTATTCTAT CATCGAGGCT ACAAGCTGAG 401 GCCTGATCGG GAATTCAGAG TTGGCATTCA GAAAGCCCAG GAAGCCATCA 451 ACAACTCAGT GGGAAGTCCT TCTTCCATTA AGCTGGAGAA CAAAGGGGAC 501 CTCTCCTTCT TAAGCAAGCA GGCTGAGAAT ATAAAAGCCC AGCAGAAGCC 551 TCAGCCCATG AAACACCTCT TACACCCCAC CAAGGGAGAG CCCAAGTGGA 601 AGGCCTCGCT CAAGAGTGAG AAGACTGTCC GCCAGCTTCT GGGGGAGCTC 651 TACGTGGACA AAGAGTATTT GGAGAAGCTC CTATTGGATG AAGACCTGAT 701 CAAAGGCACC ATGAAGGGCG GCCTGACTGT GGAGGACCTC ATCATGACGG 751 GCATCAACTA CCTGGATACT CACAGCAACT TCTGGAGGCA GCAGAAGCCG 801 ATCTACGCCA GGGAGCGGGA CCGGAAGCTG ATGCAAGAGA AATGGCTGCG 851 GGACCACAAA CGCCGTCCCT CACAGACAGC CCATTACATC CTCAAGAGCC 901 TGGAGGACAT TGATATGTTG CTCACAAGTG GCAGTGCTGA AGGGAGTCTT 951 CAGAAAGCTG AGAAAGTGCT GAAGAAGGTA CTGGAATGGA ACAAGGAAGA 1001 GGTACCCAAC AAGGATGAAC TGGTTGGAAA CTTGTATAGC TGCATAGGGA 1051 ATGCCCAGAT TGAGCTGGGG CAGATGGAGG CAGCCCTGCA GAGCCACAGA 1101 AAGGACCTGG AGATCGCCAA GGAATATGAC CTTCCTGATG CAAAATCGAG 1151 AGCCCTTGAC AACATTGGCA GAGTTTTTGC CAGAGTTGGG AAATTCCAGC 1201 AAGCCATTGA CACGTGGGAA GAAAAGATCC CTCTGGCAAA AACCACCCTG 1251 GAGAAGACCT GGCTGTTCCA CGAGATCGGC CGCTGCTACT TGGAGCTGGA 1301 CCAGGCCTGG CAGGCCCAGA ATTATGGCGA GAAGTCCCAG CAGTGTGCCG 1351 AGGAGGAAGG GGACATTGAG TGGCAACTGA ATGCCAGTGT TCTGGTGGCC 1401 CAGGCACAAG TGAAGCTGAG AGACTTCGAG TCAGCCGTGA ACAATTTTGA 1451 GAAGGCCCTG GAGAGAGCAA AGCTTGTGCA TAACAACGAG GCGCAGCAGG 1501 CCATCATCAG TGCCTTGGAC GATGCCAACA AGGGTATCAT CAGAGAACTG 1551 AGGAAAACCA ACTACGTGGA GAATCTCAAA GAAAAAAGCG AGGGAGAAGC 1601 TTCACTGTAT GAAGATAGAA TAATAACAAG AGAGAAGGAC ATGAGGAGAG 1651 TGAGAGATGA GCCCGAGAAG GTGGTGAAGC AGTGGGACCA TAGTGAGGAT 1701 GAGAAAGAGA CAGATGAGGA CGATGAGGCT TTTGGGGAAG CTCTGCAGAG 1751 CCCAGCAAGC GGAAAGCAGA GTGTGGAAGC AGGAAAAGCC AGAAGCGATT 1801 TGGGAGCAGT TGCCAAGGGC CTGTCAGGAG AATTAGGCAC AAGATCAGGA 1851 GAAACAGGCA GGAAGCTACT AGAAGCTGGC AGAAGAGAGT CAAGAGAAAT 1901 TTATAGGAGG CCTTCGGGAG AATTAGAGCA AAGACTCTCA GGAGAATTCA 1951 GCAGACAGGA ACCAGAAGAA CTAAAGAAAC TTTCAGAAGT GGGCAGAAGA 2001 GAGCCAGAAG AACTGGGAAA AACACAATTT GGAGAAATAG GAGAAACGAA 2051 AAAAACAGGA AATGAGATGG AAAAGGAATA TGAATGAAGC CATCGGTAGA 2101 GATGAGGATC AGGAAGCTGG TGTTCAGAGG GATCATGGGA TTTTATTAAA 2151 CTGGATTTTC AAGCGATTTG TCTGTTATAG GAAAAATGAG GGTTTTACTT 2201 CTGCTGCTTT CCATCACTAT TTTGCCATTA AATAGGTGTC TTTCACTCTT 2251 GCAAAAAAAA AAAAAAAAAA AAAAAAA BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 3 ORF from 69 bp to 2084 bp; peptide length: 672 Category: similarity to known protein 1 MSDPEGETLR ΞTFPSYMAEG ERLYLCGEFS KAAQSFSNAL YLQDGDKNCL 51 VARSKCFLKM GDLERSLKDA EASLQSDPAF CKGILQKAET LYTMGDFEFA 101 LVFYHRGYKL RPDREFRVGI QKAQEAINNS VGSPSSIKLE NKGDLSFLSK 151 QAENIKAQQK PQPMKHLLHP TKGEPKWKAS LKSEKTVRQL LGELYVDKEY 201 LEKLLLDEDL IKGTMKGGLT VEDLIMTGIN YLDTHΞNFWR QQKPIYARER 251 DRKLMQEKWL RDHKRRPSQT AHYILKSLED IDMLLTSGSA EGSLQKAEKV 301 LKKVLEWNKE EVPNKDELVG NLYSCIGNAQ IELGQMEAAL QSHRKDLEIA 351 KEYDLPDAKS RALDNIGRVF ARVGKFQQAI DTWEEKIPLA KTTLEKTWLF 401 HEIGRCYLEL DQAWQAQNYG EKSQQCAEEE GDIEWQLNAS VLVAQAQVKL 451 RDFESAVNNF EKALERAKLV HNNEAQQAII SALDDANKGI IRELRKTNYV 501 ENLKEKSEGE AΞLYEDRIIT REKDMRRVRD EPEKVVKQWD HSEDEKETDE 551 DDEAFGEALQ SPASGKQSVE AGKARSDLGA VAKGLSGELG TRSGETGRKL 601 LEAGRRESRE IYRRPSGELE QRLSGEFSRQ EPEELKKLSE VGRREPEELG 651 KTQFGEIGET KKTGNEMEKE YE BLASTP hits Entry AF039202_1 from database TREMBL: product: "Hsp70/Hsp90 organizing protein"; Cricetulus griseus Hsp70/Hsp90 organizing protein mRNA, complete eds. Score = 149, P = 5.3e-07, identities = 42/160, positives = 74/160 Entry AI09782_1 from database TREMBL: product: "myosin heavy chain"; Argopecten irradians myosin heavy chain mRNA, complete eds. Score = 155, P = 6. le-07, identities = 140/623, positives = 256/623 Entry S56658 from database PIR: stress-induced protein stil - soybean Score = 156, P = 9.7e-08, identities = 41/153, positives = 72/153 Alert BLASTP hits for DKFZphtes3_15hl, frame 3 No Alert BLASTP hits found Pedant information for DKFZphtes3_15hl, frame 3 Report for DKFZphtes3_15hl .3 [LENGTH] 672 [MW] 76655.61 [pi] 5.49 [HOMOL] PIR:S56658 stress-induced protein stil - soybean 6e-10 [SUPFAM] tetratricopeptide repeat homology le-07 [PROSITE] MYRISTYL 7 [PROSITE] AMIDATION 3 [ PROSITE] CAMP_PHOSPHO_SITE 4 [PROSITE] CK2_PHOSPHO_SITE 15 [PROSITE] TYR_PHOSPHO_ΞITE 1 [PROSITE] PKC_PHOSPHO_SITE 11 [PROSITE] ASN_GLYCOSYLATION 2 [KW] All_Alpha [KW] LOW_COMPLEXITY 4.76 % SEQ MSDPEGETLRΞTFPSYMAEGERLYLCGEFSKAAQSFSNALYLQDGDKNCLVARSKCFLKM SEG PRD cccccccceeeccccccccccccccccchhhhhhhhhhhhhhccccceeehhhhhhhhhh SEQ GDLERSLKDAEASLQSDPAFCKGILQKAETLYTMGDFEFALVFYHRGYKLRPDREFRVGI SEG PRD hcchhhhhhhhhhhhhccchhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccchhhhhh SEQ QKAQEAINNSVGSPSSIKLENKGDLSFLSKQAENIKAQQKPQPMKHLLHPTKGEPKWKAS SEG PRD hhhhhhhhhhhhhhhhhhhhccchhhhhhhchhhhhhhcccchhhhhhcccccccchhhh SEQ LKSEKTVRQLLGELYVDKEYLEKLLLDEDLIKGTMKGGLTVEDLIMTGINYLDTHSNFWR SEG xxxxxxxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhhhhhhhhhcccchhhhhhhhhhhhhhhhhccccccccccccc SEQ QQKPIYARERDRKLMQEKWLRDHKRRPSQTAHYILKSLEDIDMLLTSGSAEGSLQKAEKV SEG PRD cchhhhhhhhhhhhhhhhhhhhccccccchhhhhhhhhhhheeeeeccccchhhhhhhhh SEQ LKKVLEWNKEEVPNKDELVGNLYSCIGNAQIELGQMEAALQSHRKDLEIAKEYDLPDAKS SEG PRD hhhhhhhhcccccccceeecccccccchhhhhhhhhhhhhhhhhhhhhhhhhcccccchh SEQ RALDNIGRVFARVGKFQQAIDTWEEKIPLAKTTLEKTWLFHEIGRCYLELDQAWQAQNYG SEG PRD hhhccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccchhhhhhhhhhhhhhh SEQ EKSQQCAEEEGDIEWQLNAΞVLVAQAQVKLRDFESAVNNFEKALERAKLVHNNEAQQAII SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccchhhhhh SEQ SALDDANKGIIRELRKTNYVENLKEKSEGEASLYEDRIITREKDMRRVRDEPEKVVKQWD SEG x PRD hhhhccchhhhhhhhhhhhhhhhhhhcccchhhhhhhhhhhhhhhhhhhhccceeeeecc SEQ HSEDEKETDEDDEAFGEALQSPAΞGKQSVEAGKARSDLGAVAKGLSGELGTRSGETGRKL SEG xxxxxxxxxxxxx PRD ccccccccccchhhhhhhcccccccchhhhhccccccceeeeecccccccccccccchhh SEQ LEAGRRESREIYRRPSGELEQRLSGEFSRQEPEELKKLSEVGRREPEELGKTQFGEIGET SEG PRD hhhcccccceeeeccccchhhhhcccccchhhhhhhhhhhcccccccccccccccccccc SEQ KKTGNEMEKEYE SEG PRD cccccccccccc Prosite for DKFZphtes3_15hl .3 PS00001 128->132 ASN_GLYCOSYLATION PDOC00001 PS00001 438->442 ASN_GLYCOSYLATION PDOC00001 PS00004 265->269 CAMP_PHOSPHO_SITE PDOC00004 PS00004 605->609 CAMP_PHOSPHO_SITE PDOC00004 PΞ00004 613->617 CAMP_PHOSPHO_SITE PDOC00004 PS00004 636->640 CAMP_PHOΞPHO_SITE PDOC00004 PS00005 8->ll PKC_PHOSPHO_SITE PDOC00005 PS00005 66->69 PKC_PHOSPHO_SITE PDOC00005 PS00005 136->139 PKC_PHOΞPHO_SITE PDOC00005 PS00005 180->183 PKC_PHOSPHO_SITE PDOC00005 PS00005 183->186 PKC_PHOSPHO_SITE PDOC00005 PS00005 186->189 PKC_PHOSPHO_SITE PDOC00005 PS00005 214->217 PKC_PHOSPHO_SITE PDOC00005 PS00005 342->345 PKC_PHOSPHO_SITE PDOC00005 PS00005 564->567 PKC_PHOΞPHO_SITE PDOC00005 PS00005 596->599 PKC_PHOSPHO_SITE PDOC00005 PS00005 660->663 PKC_PHOSPHO_SITE PDOC00005 PS00006 2->6 CK2_PHOSPHO_SITE PDOC00006 PS00006 66->70 CK2_PHOSPHO_SITE PDOC00006 PS00006 93->97 CK2_PHOSPHO_SITE PDOC00006 PS00006 171->175 CK2_PHOSPHO_SITE PDOC00006 PS00006 220->224 CK2_PHOSPHO_SITE PDOC00006 PS00006 277->281 CK2_PHOSPHO_SITE PDOC00006 PS00006 382->386 CK2_PHOSPHO_SITE PDOC00006 PS00006 392->396 CK2_PHOSPHO_SITE PDOC00006 PS00006 481->485 CK2_PHOSPHO_SITE PDOC00006 PS00006 507->511 CK2_PHOSPHO_SITE PDOC00006 PS00006 512->516 CK2_PHOSPHO_SITE PDOC00006 PS00006 542->546 CK2_PHOSPHO_SITE PDOC00006 PS00006 548->552 CK2_PHOSPHO_SITE PDOC00006 PS00006 628->632 CK2_PHOSPHO_SITE PDOC00006 PS00006 663->667 CK2_PHOSPHO_SITE PDOC00006 PS00007 506->515 TYR_PHOSPHO_SITE PDOC00007 PS00008 119->125 MYRISTYL PDOC00008 PS00008 132->138 MYRISTYL PDOC00008 PS00008 213->219 MYRISTYL PDOC00008 PS00008 288->294 MYRISTYL PDOC00008 PS00008 320->326 MYRISTYL PDOC00008 PS00008 334->340 MYRISTYL PDOC00008 PS00008 590->596 MYRISTYL PDOC00008 PS00009 596->600 AMIDATION PDOC00009 PS00009 603->607 AMIDATION PDOC00009 PS00009 641->645 AMIDATION PDOC00009 (No Pfam data available for DKFZphtes3_15hl .3) DKFZphtes3_15ι5 group: cell structure and motility DKFZphtes3_15ι5 encodes a novel 717 amino acid protein with similarity to radial spokehead proteins . The novel protein is similar to the Chlamydomonas reinhardtn radial spokehead protein of flagella or axoneme and to the Strongylocentrotus purpuratus sea urchin spermatozoa protein p63. This protein is important for the maintenance of a planar form of sperm flagellar beating. In addition, the novel protein contains a transferrin signature 1 for iron-binding. The new protein seems to be a part of the human radial spoke heads in spermatozoa. BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in modulating the structure of the human spermatozoa radia spoke head and modulation of sperm motility in men. strong similarity to "radial spokehead" proteins complete cDNA, complete eds, 1 EST hit (from a testis library) "radial spokehead" part of flagella in Chlamydomona, this protein seems to be part of the sperm motor or tail Sequenced by GBF Locus : unknown Insert length: 2478 bp Poly A stretch at pos. 2452, polyadenylation signal at pos. 2433 1 CACCCTGGCC CGCTCCCCGC GCCCTCCACG GGTAACGGCC CCCTCTCTCG 51 GTGCTCAGAA ACCGGCGGTG TCGACAGGTG GCTCTCGCTT GGCCTCCTTG 101 TCTGCAAGCC TTTCTCCTAG AGATCTGTGC CTCCTGGCGA ACCATGGGAG 151 ACCTGCCGCC CTACCCTGAG CGCCCTGCCC AGCAGCCTCC GGGCCGGAGG 201 ACTTCTCAGG CCTCCCAGAG GCGGCACAGT CGGGACCAAG CTCAGGCCCT 251 GGCAGCGGAC CCCGAGGAGA GGCAGCAGAT ACCTCCAGAC GCCCAGCGAA 301 ACGCCCCTGG TTGGTCACAG AGGGGCAGCC TGTCCCAACA GGAGAACTTG 351 CTGATGCCCC AGGTCTTCCA GGCTGAGGAA GCCCGGCTGG GTGGCATGGA 401 GTACCCATCT GTGAACACGG GCTTTCCCTC AGAGTTCCAG CCTCAGCCTT 451 ACTCTGATGA AAGCAGGATG CAGGTCGCCG AGCTCACCAC CAGCCTAATG 501 CTGCAGCGGC TCCAGCAGGG CCAAAGCAGC CTGTTCCAGC AACTGGACCC 551 CACCTTCCAG GAGCCCCCAG TCAACCCCTT GGGCCAGTTC AACCTCTACC 601 AGACAGACCA GTTCTCTGAA GGTGCCCAGC ACGGGCCTTA CATAAGGGAT 651 GACCCTGCCC TTCAGTTCTT GCCCTCTGAG CTGGGCTTCC CACACTACAG 701 TGCCCAGGTG CCTGAGCCCG AGCCTCTGGA GCTGGCCGTG CAGAACGCCA 751 AGGCCTACCT GCTGCAGACC AGCATCAATT GCGACCTCAG CCTGTACGAG 801 CACCTGGTAA ATCTGCTGAC CAAGATCCTG AACCAGCGGC CTGAGGACCC 851 CTTGTCTGTC CTGGAGTCTC TGAACCGCAC CACGCAGTGG GAGTGGTTCC 901 ACCCCAAGCT GGACACGCTG CGGGACGACC CCGAGATGCA GCCCACCTAC 951 AAGATGGCGG AGAAACAGAA GGCGCTGTTC ACCCGGAGTG GAGGCGGCAC 1001 TGAAGGCGAA CAGGAGATGG AGGAGGAGGT GGGGGAGACA CCAGTGCCCA 1051 ACATCATGGA GACTGCCTTC TACTTCGAGC AGGCCGGCGT CGGCCTGAGC 1101 TCGGACGAGA GCTTCCGCAT TTTCCTGGCC ATGAAACAGC TGGTGGAGCA 1151 GCAGCCCATC CACACCTGTC GCTTCTGGGG CAAGATCCTG GGAATCAAAC 1201 GCAGCTACCT GGTGGCCGAG GTGGAATTCC GGGAGGGCGA GGAGGAGGCA 1251 GAGGAGGAGG AGGTGGAGGA GATGACGGAA GGTGGCGAGG TCATGGAGGC 1301 GCACGGCGAG GAGGAGGGCG AGGAGGACGA GGAGAAGGCC GTGGACATCG 1351 TCCCTAAGTC CGTATGGAAG CCGCCGCCCG TGATCCCCAA GGAGGAGAGC 1401 CGCTCAGGCG CCAACAAGTA CCTGTACTTT GTGTGCAACG AGCCGGGCCT 1451 GCCATGGACG CGGCTGCCCC ACGTCACTCC AGCCCAGATC GTGAACGCCC 1501 GAAAGATCAA GAAGTTCTTC ACAGGCTACC TGGACACGCC AGTCGTCAGC 1551 TACCCACCCT TCCCGGGCAA CGAGGCCAAC TACCTGCGGG CCCAGATAGC 1601 CCGCATCTCG GCCGCCACGC AGGTCAGCCC GCTGGGCTTC TACCAGTTTA 1651 GTGAGGAGGA GGGCGACGAG GAGGAGGAAG GTGGTGCTGG GCGCGACTCC 1701 TACGAGGAGA ACCCGGACTT CGAGGGCATC CCCGTGCTGG AGCTGGTCGA 1751 CTCCATGGCC AACTGGGTGC ATCACACACA GCACATCCTG CCGCAGGGCC 1801 GCTGCACTTG GGTGAACCCT TTGCAGAAGA CAGAGGAGGA GGAGGACCTG 1851 GGGGAGGAGG AAGAGAAGGC AGATGAGGGG CCAGAGGAGG TGGAGCAGGA 1901 GGTTGGCCCC CCACTGCTAA CGCCACTTTC AGAAGATGCA GAAATCATGC 1951 ACCTGGCACC CTGGACCACC CGCCTGTCCT GCAGCCTCTG CCCGCAGTAC 2001 TCAGTGGCCG TTGTGCGCTC CAACCTCTGG CCCGGGGCCT ATGCCTATGC 2051 CAGTGGCAAA AAGTTTGAGA ACATCTACAT CGGCTGGGGT CACAAGTACA 2101 GCCCCGAGAG CTTCAACCCG GCCCTGCCAG CCCCCATTCA ACAAGAGTAC 2151 CCCAGTGGCC CAGAGATCAT GGAGATGAGT GACCCCACAG TGGAAGAGGA 2201 GCAGGCTCTG AAAGCAGCCC AGGAACAAGC CCTGGGAGCC ACAGAGGAGG 2251 AGGAGGAGGG CGAGGAGGAG GAGGAGGGCG AGGAGACAGA TGACTGAGGC 2301 CCACCCTCTA GCCACTTTCC CCAAGCAGGT AGATAGCAAA TTTCCCCTTA 2351 GAGGTAGTTA GCATGGATTA TATTTTCACT ATGTGCTTCC TGTCCCCAGA 2401 GGGCAGGGAT AGAAAAGGAA GGCAACTGCT TCAAATAAAA TTCCTCCACG 2451 GCATTAAAAA AAAAAAAAAA AAAAAAAG BLAST Results No BLAST result Medline entries 86251010: Molecular cloning and expression of flagellar radial spoke and dynein genes of Chlamydomona 81142496: Radial spokes of Chlamydomonas flagella: polypeptide composition and phosphorylation of stalk components. 9450971: Molecular cloning and characterization of a radial spoke head protein of sea urchin sperm axonemes: involvement of the protein m the regulation of sperm motility. Peptide information for frame 3 ORF from 144 bp to 2294 bp; peptide length: 717 Category: strong similarity to known protein 1 MGDLPPYPER PAQQPPGRRT SQASQRRHSR DQAQALAADP EERQQIPPDA 51 QRNAPGWSQR GSLSQQENLL MPQVFQAEEA RLGGMEYPSV NTGFPSEFQP 101 QPYSDESRMQ VAELTTSLML QRLQQGQSSL FQQLDPTFQE PPVNPLGQFN 151 LYQTDQFΞEG AQHGPYIRDD PALQFLPSEL GFPHYSAQVP EPEPLELAVQ 201 NAKAYLLQTS INCDLSLYEH LVNLLTKILN QRPEDPLSVL ESLNRTTQWE 251 WFHPKLDTLR DDPEMQPTYK MAEKQKALFT RSGGGTEGEQ EMEEEVGETP 301 VPNIMETAFY FEQAGVGLSS DESFRIFLAM KQLVEQQPIH TCRFWGKILG 351 IKRSYLVAEV EFREGEEEAE EEEVEEMTEG GEVMEAHGEE EGEEDEEKAV 401 DIVPKSVWKP PPVIPKEESR SGANKYLYFV CNEPGLPWTR LPHVTPAQIV 451 NARKIKKFFT GYLDTPVVSY PPFPGNEANY LRAQIARISA ATQVSPLGFY 501 QFSEEEGDEE EEGGAGRDSY EENPDFEGIP VLELVDSMAN WVHHTQHILP 551 QGRCTWVNPL QKTEEEEDLG EEEEKADEGP EEVEQEVGPP LLTPLSEDAE 601 IMHLAPWTTR LSCSLCPQYS VAVVRSNLWP GAYAYASGKK FENIYIGWGH 651 KYSPESFNPA LPAPIQQEYP SGPEIMEMSD PTVEEEQALK AAQEQALGAT 701 EEEEEGEEEE EGEETDD BLASTP hits Entry U73123_l from database TREMBL: product: "radial spokehead"; Strongylocentrotus purpuratus radial spokehead mRNA, complete eds . Score = 1604, P = 7.4e-165, identities = 303/523, positives = 395/523 Entry B44498 from database PIR: radial spoke protein 6 - Chlamydomonas reinhardtn Score = 386, P = 3.4e-45, identities = 105/264, positives 138/264 Alert BLASTP hits for DKFZphtes3_15ι5, frame 3 No Alert BLASTP hits found Pedant information for DKFZphtes3_15ι5, frame 3 Report for DKFZphtes3_15ι5.3 [LENGTH] 717 [MW] 80913.61 [pi] 4.36 [HOMOL] TREMBL:U73123_1 product: "radial spokehead"; Strongylocentrotus purpuratus radial spokehead mRNA, complete eds. le-130 [PROSITE] TRANSFERRING 1 [PROSITE] MYRISTYL 5 [PROSITE] AMIDATION 2 [PROSITE] CAMP_PHOSPHO_SITE 2 [PROSITE] CK2_PHOSPHO_SITE 14 [PROSITE] TYR_PHOSPHO_SITE 1 [PROSITE] GLYCOSAMINOGLYCAN 1 [PROSITE] PKC_PHOSPHO_SITE [PROSITE] ASN SLYCOSYLATION [KW] All_Alpha [KW] LOW COMPLEXITY 21.48 % SEQ MGDLPPYPERPAQQPPGRRTSQASQRRHSRDQAQALAADPEERQQIPPDAQRNAPGWSQR SEG .... xxxxxxxxxxxx PRD ccccccccccccccccccccchhhhhhhhhhhhhhhhhcccccccccccccccccccccc SEQ GSLSQQENLLMPQVFQAEEARLGGMEYPSVNTGFPSEFQPQPYSDESRMQVAELTTSLML SEG xxxx PRD cccchhhhhhhhhhhhhhhhhhccccccccccccccccccccccchhhhhhhhhhhhhhh SEQ QRLQQGQSSLFQQLDPTFQEPPVNPLGQFNLYQTDQFSEGAQHGPYIRDDPALQFLPSEL SEG xxxxxxxxxxxxxx PRD hhhhhccccccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ GFPHYSAQVPEPEPLELAVQNAKAYLLQTSINCDLSLYEHLVNLLTKILNQRPEDPLSVL SEG PRD ccccccccccccccchhhhhhhhhhhhhhccccccchhhhhhhhhhhhhhhccccchhhh SEQ ESLNRTTQWEWFHPKLDTLRDDPEMQPTYKMAEKQKALFTRSGGGTEGEQEMEEEVGETP SEG xxxxxxxxxxxxxxxx .. PRD hhhchhhhhccccccccccccccccchhhhhhhhhhhhhhhcccccchhhhhhhhhcccc SEQ VPNIMETAFYFEQAGVGLSSDESFRIFLAMKQLVEQQPIHTCRFWGKILGIKRSYLVAEV SEG xxx PRD ccchhhhhhhhhhccccccchhhhhhhhhhhhhhhhhccchhhhhhhhcccchhhhhhhh SEQ EFREGEEEAEEEEVEEMTEGGEVMEAHGEEEGEEDEEKAVDIVPKSVWKPPPVIPKEEΞR SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhcccccccccccccchhhhheeeeecccccccccccccccccc SEQ SGANKYLYFVCNEPGLPWTRLPHVTPAQIVNARKIKKFFTGYLDTPVVSYPPFPGNEANY SEG PRD cccceeeeeeeccccccccccccccchhhhhhhhhhhhhhcccccccccccccccchhhh SEQ LRAQIARISAATQVSPLGFYQFSEEEGDEEEEGGAGRDSYEENPDFEGIPVLELVDSMAN SEG xxxxxxxxxxxxx PRD hhhhhhhhhhhhccccccceeeeccccccccccccccccccccccccccceeeecchhhh SEQ WVHHTQHILPQGRCTWVNPLQKTEEEEDLGEEEEKADEGPEEVEQEVGPPLLTPLSEDAE SEG xxxxxxxxxxxxxxxxxxxxxxxxxxx PRD hhhcccccccccceeechhhhhhhhhccccchhhhhcccccccccccccccccccccccc SEQ IMHLAPWTTRLSCSLCPQYSVAVVRSNLWPGAYAYASGKKFENIYIGWGHKYSPESFNPA SEG PRD cccccccccccccccccccceeeeeeccccceeeecccccceeeeeeccccccccccccc SEQ LPAPIQQEYPSGPEIMEMSDPTVEEEQALKAAQEQALGATEEEEEGEEEEEGEETDD SEG xxxxxxxxxxxxxx ... xxxxxxxxxxxxxx ... PRD cccccccccccccceeeeccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccc Prosite for DKFZphtes3_15ι5.3 PS00001 244->248 ASN_GLYCOSYLATION PDOC00001 PS00002 282->286 GLYCOSAMINOGLYCAN PDOC00002 PS00004 18->22 CAMP_PHOSPHO_SITE PDOC00004 PS00004 26->30 CAMP_PHOSPHO_SITE PDOC00004 PS00005 24->27 PKC_PHOSPHO_SITE PDOC00005 PS00005 58->61 PKC_PHOSPHO_SITE PDOC00005 PS00005 258->261 PKC_PHOSPHO_SITE PDOC00005 PS00005 268->271 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 323->326 PKC_PHOSPHO_SITE PDOC00005 PS00005 341->344 PKC_PHOSPHO_SITE PDOC00005 PS00005 608->611 PKC_PHOSPHO_SITE PDOC00005 PS00005 637->640 PKC_PHOSPHO_SITE PDOC00005 PS00006 64->68 CK2_PHOSPHO_SITE PDOC00006 PS00006 137->141 CK2 PHOSPHO SITE PDOC00006 ?! ?: ?! ?: ?: to ro ro to ro to ro 1 1 1 1 1 1 en cn ω cn en ω en en 131313 K K K cn cn en en en en Ui SO 00 DKFZphtes3_15jl8 group: testes derived DKFZphtes3_15j 18 encodes a novel 148 amino acid protein without similarity to known proteins. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . unknown complete cDNA, complete eds, few EST hits Sequenced by GBF Locus : unknown Insert length: 905 bp Poly A stretch at pos. 839, polyadenylation signal at pos. 815 1 GTGATTCATA TGCTTCCATA GCAGGTGTCT GCTTCTGAGC CAAGCTCCCA 51 GGGCAGCGGA GCAGGCACCA ACCAGCATCC CAGGGGAGGG CACAGCTTGT 101 CCAGCTGGGA TGTTTGGGTG CCCTGTGAGA TGCCCCAAGC CACCAACCCA 151 GCTTATCTCA GGAGAAGCCT CGGCGGCCCG TCTGCCGGCC TGGAGAGATG 201 TGCTACAGCA GCCGGGGGTG GGGGGAGAGG GTGGGCTTAG AATCTCTTGG 251 CAGGGAGCCC CCAAGAGCAG GGTGAGACCT GCCTTCATTT CACCTGTCCC 301 CTTCACAGTT CTGCAAAGCC AGCATTATCA TCCCTTTTCA GAAGGAGTGG 351 GCACTCAGGT GGAATGCCTC ACCCCAGTCC TGCGGCTGGA AAGCGATATG 401 GCCAGGACTG CACCCCACCC CTCATCCCTG CACCCCTTCC CTGCCTGGGA 451 TTCCTCCAGC CCTGTGCACT GTGGAGCGCC TCTGCCTTCC GCTCATGGAG 501 GTTTCCCAAG GGCACGCGCT GAGGGCAGCT GGTCTCAGCC TGGGGCCGGG 551 TCCTAGTAAC TGTCTCTCTT TGCTTTCCAG CCAGTGTTTT GGGGTTTGAA 601 GTTGGAATCT TCAGCTACTG TCAAGAACAG CCACAAAAAT GTGTCACGAT 651 CAAGATCTTT GAGAGTCCAC CAATCAGGAG GCGTCTGTGA CAGTCGCTGT 701 CTTCTCAGAA CAGAATCCAC ACCCAGGATT CAACCCAAAT GATTTCTCAT 751 CAGGTGATTC TTGGTTGTAG CAAAGTTCAT GTGAATGTGG GTGAGTTTCT 801 GTTATGAATG TGGTCAATAA ATGTTATTTG TGAAACTCTA AAAAAAAAAA 851 AAAAAAAAAG GGCGGCCGCT CTAGAGGATC CAAGCTTACG TACGCGAAAA 901 AAAAG BLAST Results No BLAST result Medlme entries No Medline entry Peptide information for frame 2 ORF from 110 bp to 553 bp; peptide length: 148 Category: putative protein 1 MFGCPVRCPK PPTQLISGEA SAARLPAWRD VLQQPGVGGE GGLRISWQGA 51 PKSRVRPAFI SPVPFTVLQS QHYHPFSEGV GTQVECLTPV LRLESDMART 101 APHPSSLHPF PAWDSSSPVH CGAPLPSAHG GFPRARAEGS WSQPGAGS BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_15j 18, frame 2 No Alert BLASTP hits found Pedant information for DKFZphtes3_15 18, frame 2 Report for DKFZphtes3_15 18.2 [LENGTH] 148 [MW] 15665.78 [pl] 8.91 [PROSITE] MYRISTYL 3 [PROSITE] CK2_PHOSPHO_SITE [KW] Irregular SEQ MFGCPVRCPKPPTQLISGEASAARLPAWRDVLQQPGVGGEGGLRISWQGAPKSRVRPAFI PRD cccccccccccccccccccccccchhhhhhhccccccccccceeeeeccccccccccccc SEQ SPVPFTVLQSQHYHPFSEGVGTQVECLTPVLRLESDMARTAPHPSSLHPFPAWDSSSPVH PRD cccceeeeeccccccccccccccccccchhhhhhhhcccccccccccccccccccccccc SEQ CGAPLPSAHGGFPRARAEGSWSQPGAGS PRD cccccccccccccccccccccccccccc Prosite for DKFZphtes3_15j 18.2 PS00006 82->86 CK2_PHOSPHO_SITE PDOC00006 PS00008 38->44 MYRISTYL PDOC00008 PS00008 42->48 MYRISTYL PDOC00008 PS00008 49->55 MYRISTYL PDOC00008 (No Pfam data available for DKFZphtes3_15j 18.2) DKFZphtes3_15j3 group: nucleic acid management DKFZphtes3_15 3 encodes a novel 743 amino acid protein with similarity to proteins with unknown function. The novel protein contains a RNA recognition motif, predicted by Pfam and therefore binds to RNA. The protein is similar to YGR276c, a ribonuclease H of S. cerevisiae. Thus, the protein seems to a new RNA-modificating protein. The new protein can find application in modulating the RNA metabolism in human cells and as a tool for biotechnologic manipulations . "44M2.3"; product, differences to genmodel, similarity to ribonuclease H complete cDNA, complete eds, EST hits YGR276c = ribonuclease H differences to genmodel of 44M2.3 Sequenced by GBF Locus: /map="16pll .2" Insert length: 2695 bp Poly A stretch at pos. 2601, polyadenylation signal at pos. 2579 1 GCGGTTGTTG TTGGCAGCTG TGGCTAAGGA GGGGAGAACC TCTGCTCCCC 51 GCCCGTCTTC TCTTCTGCGT TTCCCGGGCT AGGGGGCGTG GGGAGTGGTT 101 TTAGGCGGCG AAGCCGCTCG GCAGCACCTT CCTTCTTTGC CAGGCAGACG 151 CCCGTTGTAG CCGTTGGGGA ACCGTTGAGA ATCCGCCATG GAGCCAGAGA 201 GGGAAGGGAC CGAGAGACAC CCCAGGAAGG TCAGGGAAAG CAGGCAGGCC 251 CCAAATAAGC TGGTCGGGGC AGCTGAGGCG ATGAAAGCCG GTTGGGATCT 301 CGAGGAGAGT CAGCCCGAGG CCAAGAAAGC CCGCTTATCT ACCATTTTAT 351 TTACTGACAA CTGTGAAGTA ACCCATGACC AGCTGTGTGA ATTGCTGAAG 401 TATGCAGTTC TGGGCAAATC CAATGTTCCA AAACCCAGCT GGTGCCAGCT 451 TTTTCATCAA AACCACCTAA ACAACGTAGT GGTTTTTGTT CTGCAGGGAA 501 TGAGTCAGCT ACACTTTTAC AGGTTCTATT TGGAGTTTGG ATGTCTTCGA 551 AAAGCATTCA GACATAAATT CCGCTTGCCT CCACCATCAT CTGATTTTCT 601 AGCTGATGTT GTTGGGCTAC AAACTGAACA AAGAGCTGGA GATCTGCCCA 651 AGACAATGGA AGGGCCTTTA CCTTCTAATG CAAAAGCCGC CATCAACCTT 701 CAGGATGATC CCATCATTCA AAAGTATGGC TCTAAGAAAG TGGGCTTGAC 751 CAGATGCCTT CTGACAAAGG AGGAAATGAG AACGTTTCAC TTTCCATTAC 801 AAGGTTTTCC TGATTGTGAA AACTTTTTAC TTACCAAATG TAATGGTTCT 851 ATAGCAGACA ATAGTCCTCT CTTTGGACTT GACTGTGAAA TGTGCCTCAC 901 ATCCAAGGGG AGAGAGCTAA CACGCATCTC ACTGGTTGCT GAAGGAGGCT 951 GCTGTGTTAT GGATGAACTG GTCAAACCTG AAAACAAGAT TCTGGACTAC 1001 CTCACCAGCT TTTCGGGAAT CACGAAGAAG ATTCTTAACC CAGTGACGAC 1051 CAAACTCAAA GATGTACAGA GGCAGTTAAA AGCACTGCTT CCTCCTGATG 1101 CTGTGTTAGT GGGCCACTCC TTAGATTTGG ATCTCAGAGC ACTGAAAATG 1151 ATACATCCAT ATGTTATTGA TACATCGTTG CTTTATGTCA GAGAGCAGGG 1201 CAGAAGATTT AAGCTCAAGT TCTTAGCCAA AGTTATTTTG GGGAAGGATA 1251 TACAGTGTCC AGACAGACTT GGTCATGATG CCACAGAAGA TGCTAGAACA 1301 ATCCTTGAAT TGGCTCGGTA TTTCCTTAAG CATGGCCCAA AAAAGATTGC 1351 AGAACTAAAT CTAGAAGCAC TAGCTAATCA CCAAGAAATA CAAGCAGCAG 1401 GCCAAGAGCC TAAAAACACA GCAGAAGTAC TTCAGCACCC AAACACAAGT 1451 GTTTTAGAAT GCTTGGATTC AGTGGGTCAG AAGCTTCTTT TTTTGACCCG 1501 GGAGACAGAT GCTGGTGAAC TTCCATCTTC CAGAAATTGT CAAACTATTA 1551 AGTGTCTTTC AAATAAAGAG GTTCTTGAGC AGGCCAGAGT GGAAATCCCC 1601 CTGTTTCCCT TCAGCATTGT TCAGTTCTCT TTTAAGGCCT TTTCACCTGT 1651 CCTCACTGAG GAGATGAACA AAAGGATGAG GATCAAGTGG ACAGAGATAT 1701 CAACTGTCTA TGCTGGGCCA TTTAGCAAAA ATTGCAATCT CAGGGCTCTG 1751 AAGAGGCTGT TTAAAAGCTT TGGCCCAGTC CAGTCAATGA CTTTTGTTCT 1801 TGAAACCCGT CAGGTGCAGA GGCCTGTGAC AGAGCTCACG CTTGATTGTG 1851 ACACCCTCGT GAATGAGCTG GAAGGAGATT CTGAAAACCA AGGCTCTATA 1901 TATCTGTCTG GAGTGAGTGA AACCTTCAAA GAACAGCTAT TGCAGGAGCC 1951 CCGCCTCTTT CTTGGCCTGG AAGCTGTGAT CTTGCCTAAA GATCTTAAAA 2001 GTGGAAAGCA GAAAAAATAC TGTTTCCTGA AATTCAAAAG TTTTGGCAGT 2051 GCCCAGCAGG CCCTCAACAT TCTCACAGGC AAGGACTGGA AGCTGAAAGG 2101 CAGGCATGCC CTAACCCCCA GGCACCTCCA TGCCTGGCTC AGAGGCTTAC 2151 CACCTGAATC AACAAGGCTC CCAGGGCTTC GTGTTGTACC TCCCCCCTTT 2201 GAACAGGAGG CCTTGCAGAC TCTGAAACTG GACCACCCGA AGATAGCAGC 2251 CTGGCGCTGG AGCCGGAAGA TTGGAAAGCT CTACAACAGC TTGTGCCCGG 2301 GCACTCTCTG CCTCATCCTG CTGCCAGGAA CCAAGAGCAC TCATGGTTCA 2351 CTCTCTGGTC TAGGACTGAT GGGAATAAAA GAGGAAGAAG AAAGCGCTGG 2401 CCCAGGCCTG TGTTCGTGAG TCGGCCTGCC ATGTTTCCAT GTGCCATTTC 2451 TTACCCCTTG TAGGCAATGG CAAAGAATGT GGTCAGGCTG TAGCCTCCCC 2501 AACCAGCAGA CAGTTTTATG GAAACTTGGT ATAGCAGCTA AAAGAGTTTA 2551 GTTTGTTTAT ATGGCATGTA TAAGTTTTCA ATAAATGCCT AAAGTTCAAG 2601 CATAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 2651 AGGGCGGCCG CTCTAAAGGA TCCAAGCTTA CGTACGCGAA AAAAG BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 2 ORF from 188 bp to 2416 bp; peptide length: 743 Category: similarity to known protein 1 MEPEREGTER HPRKVRESRQ APNKLVGAAE AMKAGWDLEE SQPEAKKARL 51 STILFTDNCE VTHDQLCELL KYAVLGKSNV PKPSWCQLFH QNHLNNVVVF 101 VLQGMSQLHF YRFYLEFGCL RKAFRHKFRL PPPSSDFLAD VVGLQTEQRA 151 GDLPKTMEGP LPSNAKAAIN LQDDPIIQKY GSKKVGLTRC LLTKEEMRTF 201 HFPLQGFPDC ENFLLTKCNG SIADNSPLFG LDCEMCLTSK GRELTRISLV 251 AEGGCCVMDE LVKPENKILD YLTSFSGITK KILNPVTTKL KDVQRQLKAL 301 LPPDAVLVGH SLDLDLRALK MIHPYVIDTS LLYVREQGRR FKLKFLAKVI 351 LGKDIQCPDR LGHDATEDAR TILELARYFL KHGPKKIAEL NLEALANHQE 401 IQAAGQEPKN TAEVLQHPNT SVLECLDSVG QKLLFLTRET DAGELPSSRN 451 CQTIKCLSNK EVLEQARVEI PLFPFSIVQF SFKAFSPVLT EEMNKRMRIK 501 WTEISTVYAG PFSKNCNLRA LKRLFKSFGP VQSMTFVLET RQVQRPVTEL 551 TLDCDTLVNE LEGDSENQGS IYLSGVSETF KEQLLQEPRL FLGLEAVILP 601 KDLKSGKQKK YCFLKFKSFG SAQQALNILT GKDWKLKGRH ALTPRHLHAW 651 LRGLPPESTR LPGLRVVPPP FEQEALQTLK LDHPKIAAWR WSRKIGKLYN 701 SLCPGTLCLI LLPGTKSTHG SLSGLGLMGI KEEEESAGPG LCS BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_15j3, frame 2 TREMBL:AC004381_4 gene: "44M2.3"; product: "Unknown gene product"; Homo sapiens Chromosome 16 BAC clone CIT987SK-44M2, complete sequence., N = 2, Score = 1827, P = 2.1e-284 TREMBL:AF016430_4 gene: "C05C8.5"; Caenorhabditis elegans cosmid C05C8., N = 2, Score = 370, P = 1.7e-34 PIR:S64609 hypothetical protein YGR276c - yeast (Saccharomyces cerevisiae), N = 2, Score = 334, P = 1.8e-27 TREMBLNEW :SPAC637_9 gene: "SPAC637.09"; product: "putative exonuclease"; S. pombe chromosome I cosmid c637., N = 3, Score = 326, P = 2.8e-27 >TREMBL:AC004381_4 gene: "44M2.3"; product: "Unknown gene product"; Homo sapiens Chromosome 16 BAC clone CIT987SK-44M2, complete sequence. Length = 547 HSPs: Score = 1827 (274.1 bits), Expect = 2.1e-284, Sum P(2) = 2.1e-284 Identities = 358/373 (95%), Positives = 358/373 (95%) Query: 105 MSQLHFYRFYLEFGCLRKAFRHKFRLPPPSSDFLADVVGLQTEQRAGDLPKTMEGPLPSN 164 MSQLHFYRFYLEFGCLRKAFRHKFRLPPPSSDFLADVVGLQTEQRAGDLPKTMEGPLPΞN Sbjct: 1 MSQLHFYRFYLEFGCLRKAFRHKFRLPPPSSDFLADVVGLQTEQRAGDLPKTMEGPLPΞN 60 Query: 165 AKAAINLQDDPIIQKYGSKKVGLTRCLLTKEEMRTFHFPLQGFPDCENFLLTKCNGSIAD 224 AKAAINLQDDPIIQKYGSKKVGLTRCLLTKEEMRTFHFPLQGFPDCENFLLTKCNGSIAD Sbjct: 61 AKAAINLQDDPIIQKYGSKKVGLTRCLLTKEEMRTFHFPLQGFPDCENFLLTKCNGSIAD 120 Query: 225 NSPLFGLDCEM CLTSKGRELTRISLVAEGGCCVMDELVKPENKIL 269 NSPLFGLDCEM CLTSKGRELTRISLVAEGGCCVMDELVKPENKIL Sbjct: 121 NSPLFGLDCEMARTTFNFSIGVLQAECLTSKGRELTRISLVAEGGCCVMDELVKPENKIL 180 Query: 270 DYLTSFSGITKKILNPVTTKLKDVQRQLKALLPPDAVLVGHSLDLDLRALKMIHPYVIDT 329 DYLTSFSGITKKILNPVTTKLKDVQRQLKALLPPDAVLVGHSLDLDLRALKMIHPYVIDT Sbjct: 181 DYLTSFSGITKKILNPVTTKLKDVQRQLKALLPPDAVLVGHSLDLDLRALKMIHPYVIDT 240 Query: 330 SLLYVREQGRRFKLKFLAKVILGKDIQCPDRLGHDATEDARTILELARYFLKHGPKKIAE 389 SLLYVREQGRRFKLKFLAKVILGKDIQCPDRLGHDATEDARTILELARYFLKHGPKKIAE Sbjct: 241 SLLYVREQGRRFKLKFLAKVILGKDIQCPDRLGHDATEDARTILELARYFLKHGPKKIAE 300 Query: 390 LNLEALANHQEIQAAGQEPKNTAEVLQHPNTSVLECLDΞVGQKLLFLTRETDAGELPSSR 449 LNLEALANHQEIQAAGQEPKNTAEVLQHPNTSVLECLDSVGQKLLFLTRETDAGELPSΞR Sbjct: 301 LNLEALANHQEIQAAGQEPKNTAEVLQHPNTSVLECLDSVGQKLLFLTRETDAGELPSSR 360 Query: 450 NCQTIKCLSNKEV 462 NCQTIKCLSNKEV Sbjct: 361 NCQTIKCLSNKEV 373 Score = 929 (139.4 bits), Expect = 2.1e-284, Sum P(2) = 2.1e-284 Identities : = 175/179 (97%), Positives = 177/179 (98%) Query: 538 LETRQVQRPVTELTLDCDTLVNELEGDSENQGSIYLSGVSETFKEQLLQEPRLFLGLEAV 597 L ++VQRPVTELTLDCDTLVNELEGDSENQGSIYLSGVSETFKEQLLQEPRLFLGLEAV Sbjct: 368 LSNKEVQRPVTELTLDCDTLVNELEGDSENQGSIYLSGVSETFKEQLLQEPRLFLGLEAV 427 Query: 598 ILPKDLKSGKQKKYCFLKFKSFGSAQQALNILTGKDWKLKGRHALTPRHLHAWLRGLPPE 657 ILPKDLKSGKQKKYCFLKFKSFGSAQQALNILTGKDWKLKGRHALTPRHLHAWLRGLPPE Sbjct: 428 ILPKDLKSGKQKKYCFLKFKSFGSAQQALNILTGKDWKLKGRHALTPRHLHAWLRGLPPE 487 Query: 658 STRLPGLRVVPPPFEQEALQTLKLDHPKIAAWRWSRKIGKLYNSLCPGTLCLILLPGTK 716 STRLPGLRVVPPPFEQEALQTLKLDHPKIAAWRWSRKIGKLYNSLCPGTLCLILLPGTK Sbjct: 488 STRLPGLRVVPPPFEQEALQTLKLDHPKIAAWRWSRKIGKLYNΞLCPGTLCLILLPGTK 546 Pedant information for DKFZphtes3_15j3, frame 2 Report for DKFZphtes3_15j3.2 [LENGTH] 743 [MW] 83536.58 [pi] 8.87 [HOMOL] TREMBL:AC004381_4 gene: "44M2.3"; product: "Unknown gene product"; Homo sapiens Chromosome 16 BAC clone CIT987SK-44M2, complete sequence. 0.0 [FUNCAT] 01.03.16 polynucleotide degradation [S. cerevisiae, YGR276c] 4e-30 [ FUNCAT] 99 unclassified proteins [S. cerevisiae, YLR107w] 3e-13 [FUNCAT] 05.04 translation (initiation, elongation and termination) [S. cerevisiae, YGL094C] le-10 [FUNCAT] 04.05.05 mrna processing (5'-end, 3'-end processing and mrna degradation) [S. cerevisiae, YGL094C] le-10 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YOL080C] 2e-10 [PROSITE] MYRISTYL 5 [PROSITE] AMIDATION 1 [PROSITE] CK2_PHOΞPHO_SITE 8 [PROSITE] TYR_PHOSPHO_SITE 1 [PROSITE] GLYCOSAMINOGLYCAN 1 [PROSITE] PKC_PHOSPHO_SITE 16 (PROSITE] AΞN_GLYCOSYLATION 2 [PFAM] RNA recognition motif, (aka RRM, RBD, or RNP domain) [KW] Alpha_Beta SEQ MEPEREGTERHPRKVRESRQAPNKLVGAAEAMKAGWDLEESQPEAKKARLSTILFTDNCE PRD ccchhhhhccccchhhhhhhhcchhhhhhhhhhccccccccccchhhhhhccccccccce SEQ VTHDQLCELLKYAVLGKSNVPKPSWCQLFHQNHLNNVVVFVLQGMSQLHFYRFYLEFGCL PRD eehhhhhhhhhhhhhcccccccccceeeeccccccceeeeeeecchhhhhhhhhhhhhhh SEQ RKAFRHKFRLPPPΞSDFLADVVGLQTEQRAGDLPKTMEGPLPSNAKAAINLQDDPIIQKY PRD hhhhhhhhccccccccchhhhhhhhhhhhccccccccccccccchhhhhhhhcccccccc SEQ GSKKVGLTRCLLTKEEMRTFHFPLQGFPDCENFLLTKCNGSIADNSPLFGLDCEMCLTSK PRD ccccccchhhhhhhhhhhhhhccccccccccceeeeccccccccccceeeeccccccccc SEQ GRELTRISLVAEGGCCVMDELVKPENKILDYLTSFSGITKKILNPVTTKLKDVQRQLKAL PRD cchhhhheeeecccceeeeeeeccccceeecccccccccccccccccchhhhhhhhhhhh SEQ LPPDAVLVGHSLDLDLRALKMIHPYVIDTSLLYVREQGRRFKLKFLAKVILGKDIQCPDR PRD hccceeeecccchhhhhhhhhhhhccccceeeeccccccchhhhhhhhhhhhhhcccccc SEQ LGHDATEDARTILELARYFLKHGPKKIAELNLEALANHQEIQAAGQEPKNTAEVLQHPNT PRD ccccchhhhhhhhhhhhhhhhcccceeeeehhhhhhhhhhhhhhccccccceeeeecccc SEQ SVLECLDSVGQKLLFLTRETDAGELPSSRNCQTIKCLSNKEVLEQARVEIPLFPFSIVQF PRD ceeeeeeccccceeeeeecccccccccccccceeeeecchhhhhhhhhhccccccceeee SEQ SFKAFSPVLTEEMNKRMRIKWTEISTVYAGPFSKNCNLRALKRLFKSFGPVQSMTFVLET PRD eeeceeeehhhhhhhhhhhhheeeeeecccccccchhhhhhhhhhhccccceeeehhhhh SEQ RQVQRPVTELTLDCDTLVNELEGDSENQGSIYLSGVSETFKEQLLQEPRLFLGLEAVILP PRD cccccccccccccchhhhhhcccccccccccccccchhhhhhhhhhhhcccccceeeeec SEQ KDLKSGKQKKYCFLKFKSFGSAQQALNILTGKDWKLKGRHALTPRHLHAWLRGLPPESTR PRD ccccccccceeeeeeeecccchhhhhhhhhccccccccccccccchhhhhhccccccccc SEQ LPGLRVVPPPFEQEALQTLKLDHPKIAAWRWSRKIGKLYNSLCPGTLCLILLPGTKSTHG PRD ccccccccccchhhhhhhhhhcchhhhhhhhhhhhhheeeeccccceeeeeccccccccc SEQ SLSGLGLMGIKEEEESAGPGLCS PRD cccccccchhhhhhccccccccc Prosite for DKFZphtes3_15j3.2 PS00001 219->223 ASN_GLYCOSYLATION PDOC00001 PS00001 419->423 ASN_GLYCOSYLATION PDOC00001 PS00002 723->727 GLYCOSAMINOGLYCAN PDOC00002 PS00005 8->ll PKC_PHOSPHO_SITE PDOC00005 PS00005 182->185 PKC_PHOSPHO_SITE PDOC00005 PS00005 238->241 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 279->282 PKC_PHOΞPHO_SITE PDOC00005 PΞ00005 287->290 PKC_PHOSPHO_SITE PDOC00005 PS00005 447->450 PKC_PHOSPHO_SITE PDOC00005 PS00005 453->456 PKC_PHOSPHO_SITE PDOC00005 PS00005 458->461 PKC_PHOSPHO_SITE PDOC00005 PS00005 481->484 PKC_PHOSPHO_SITE PDOC00005 PS00005 579->582 PKC_PHOΞPHO_SITE PDOC00005 PS00005 605->608 PKC_PHOSPHO_SITE PDOC00005 PS00005 630->633 PKC_PHOSPHO_SITE PDOC00005 PS00005 643->646 PKC_PHOSPHO_SITE PDOC00005 PS00005 658->661 PKC_PHOΞPHO_SITE PDOC00005 PS00005 678->681 PKC_PHOSPHO_SITE PDOC00005 PS00005 692->695 PKC_PHOSPHO_SITE PDOC00005 PS00006 41->45 CK2_PHOSPHO_SITE PDOC00006 PS00006 193->197 CK2_PHOSPHO_SITE PDOC00006 PS00006 221->225 CK2_PHOSPHO_SITE PDOC00006 PS00006 371->375 CK2_PHOSPHO_SITE PDOC00006 PS00006 421->425 CK2_PHOSPHO_SITE PDOC00006 PS00006 458->462 CK2_PHOSPHO_SITE PDOC00006 PS00006 579->583 CK2_PHOSPHO_SITE PDOC00006 PS00006 630->634 CK2_PHOSPHO_SITE PDOC00006 PS00007 370->379 TYR_PHOSPHO_SITE PDOC00007 PS00008 27->33 MYRISTYL PDOC00008 PS00008 186->192 MYRISTYL PDOC00008 PS00008 575->581 MYRISTYL PDOC00008 PS00008 714->720 MYRISTYL PDOC00008 PS00008 720->726 MYRISTYL PDOC00008 PS00009 337->341 AMIDATION PDOC00009 Pfam for DKFZphtes3_15j3.2 HMM_NAME RNA recognition motif, (aka RRM, RBD, or RNP domain) HMM *IYVGNLPWDtTEEDLrDlFsQFGpIvsIrMMrDReTGRSRGFAFVEFED IY+ +++ +T +E+L + + F + + + +++D G+ + ++F +F++ Query 571 IYLSGVS-ETFKEQLLQEPRLFLGLEAVILPKDLKSGKQKKYCFLKFKS 618 HMM EEDAekAIdeMNG..meFmGRrlRV* +A+ A+ + G ++ GR + Query 619 FGSAQQALNILTGKDWKLKGRHALT 643 DKFZphtes3 15kll group: signal transduction DKFZphtes3_15kll encodes a novel 958 amino acid protein C-terminal identical with human KIAA0781 protein and high similarity to protein kinases. The novel protein contains a protein kinase ATP-binding region signature and a serine/threomne protein kinase active-site signature. The related murine kinase was cloned from the myocardium of the developing heart. The new protein can find application m modulation of intracellular signal pathways dependent on this kinase. KIAA0781, 5' extension complete cDNA, complete eds, potential start at Bp 97, EST hits Sequenced by GBF Locus: /map="ll" Insert length: 4868 bp Poly A stretch at pos. 4798, polyadenylation signal at pos. 4776 1 GAGCAAGCGG AGCGGCCGTC GCCCAAGCCA AGCCGCGCTG CCAACCCTCC 51 CGCCCGCCCG CGCTCCTGTC CGCCGTGTCT AGCAGCGGGG CCCAGCATGG 101 TCATGGCGGA TGGCCCGAGG CACTTGCAGC GCGGGCCGGT CCGGGTGGGG 151 TTCTACGACA TCGAGGGCAC GCTGGGCAAG GGCAACTTCG CTGTGGTGAA 201 GCTGGGGCGG CACCGGATCA CCAAGACGGA GGTGGCAATA AAAATAATCG 251 ATAAGTCTCA GCTGGATGCA GTGAACCTTG AGAAAATCTA CCGAGAAGTA 301 CAAATAATGA AAATGTTAGA CCACCCTCAC ATAATCAAAC TTTATCAGGT 351 AATGGAGACC AAAAGTATGT TGTACCTTGT GACAGAATAT GCCAAAAATG 401 GAGAAATTTT TGACTATCTT GCTAATCATG GCCGGTTAAA TGAGTCTGAA 451 GCCAGGCGAA AATTCTGGCA AATCCTGTCT GCTGTTGATT ATTGTCATGG 501 TCGGAAGATT GTGCACCGTG ACCTCAAAGC TGAAAATCTC CTGCTGGATA 551 ACAACATGAA TATCAAAATA GCAGATTTCG GTTTTGGAAA TTTCTTTAAA 601 AGTGGTGAAC TGCTGGCAAC ATGGTGTGGC AGCCCCCCTT ATGCAGCCCC 651 AGAAGTCTTT GAAGGGCAGC AGTATGAAGG ACCACAGCTG GACATCTGGA 701 GTATGGGAGT TGTTCTTTAT GTCCTTGTCT GTGGAGCTCT GCCCTTTGAT 751 GGACCGACTC TTCCAATTTT GAGGCAGAGG GTTCTGGAAG GAAGATTCCG 801 GATTCCGTAT TTCATGTCAG AAGATTGCGA GCACCTTATC CGAAGGATGT 851 TGGTCCTAGA CCCATCCAAA CGGCTAACCA TAGCCCAAAT CAAGGAGCAT 901 AAATGGATGC TCATAGAAGT TCCTGTCCAG AGACCTGTTC TCTATCCACA 951 AGAGCAAGAA AATGAGCCAT CCATCGGGGA GTTTAATGAG CAGGTTCTGC 1001 GACTGATGCA CAGCCTTGGA ATAGATCAGC AGAAAACCAT TGAGTCTTTG 1051 CAGAACAAGA GCTATAACCA CTTTGCTGCC ATTTATTTCT TGTTGGTGGA 1101 GCGCCTGAAA TCACATCGGA GCAGTTTCCC AGTGGAGCAG AGACTTGATG 1151 GCCGCCAGCG TCGGCCTAGC ACCATTGCTG AGCAAACAGT TGCCAAGGCA 1201 CAGACTGTGG GGCTCCCAGT GACCATGCAT TCACCGAACA TGAGGCTGCT 1251 GCGATCTGCC CTCCTCCCCC AGGCATCCAA CGTGGAGGCC TTTTCATTTC 1301 CAGCATCTGG CTGTCAGGCG GAAGCTGCAT TCATGGAAGA AGAGTGTGTG 1351 GACACTCCAA AGGTCAATGG CTGTCTGCTT GACCCTGTGC CTCCTGTCCT 1401 GGTGCGGAAG GGATGCCAGT CACTGCCCAG CAACATGATG GAGACCTCCA 1451 TTGACGAAGG GCTGGAGACA GAAGGAGAGG CCGAGGAAGA CCCCGCTCAT 1501 GCCTTTGAGG CATTTCAGTC CACACGCAGC GGGCAGAGAC GGCACACTCT 1551 GTCAGAAGTG ACCAATCAAC TGGTCGTGAT GCCTGGGGCA GGGAAAATTT 1601 TCTCCATGAA TGACAGCCCC TCCCTTGACA GTGTGGACTC TGAGTATGAT 1651 ATGGGGTCTG TTCAGAGGGA CCTGAACTTT CTGGAAGACA ACCCTTCCCT 1701 TAAGGACATC ATGTTAGCCA ATCAGCCTTC ACCCCGCATG ACATCTCCCT 1751 TCATAAGCCT GAGACCTACC AACCCAGCCA TGCAGGCTCT GAGCTCCCAG 1801 AAACGAGAGG TCCACAACAG GTCTCCAGTG AGCTTCAGAG AGGGCCGCAG 1851 AGCATCAGAT ACCTCCCTCA CCCAGGGAAT TGTAGCATTT AGACAACATC 1901 TTCAGAATCT GGCTAGAACC AAAGGAATTC TAGAGTTGAA CAAAGTGCAG 1951 TTGTTGTATG AACAAATAGG ACCGGAGGCA GACCCTAACC TGGCGCCGGC 2001 GGCTCCTCAG CTCCAGGACC TTGCTAGCAG CTGCCCTCAG GAAGAAGTTT 2051 CTCAGCAGCA GGAAAGCGTC TCCACTCTCC CTGCCAGCGT GCATCCCCAG 2101 CTGTCCCCAC GGCAGAGCCT GGAGACCCAG TACCTGCAGC ACAGACTCCA 2151 GAAGCCCAGC CTTCTGTCAA AGGCCCAGAA CACCTGTCAG CTTTATTGCA 2201 AAGAACCACC GCGGAGCCTT GAGCAGCAGC TGCAGGAACA TAGGCTCCAG 2251 CAGAAGCGAC TCTTTCTTCA GAAGCAGTCT CAACTGCAGG CCTATTTTAA 2301 TCAGATGCAG ATAGCAGAGA GCTCCTACCC ACAGCCAAGT CAGCAGCTGC 2351 CCCTTCCCCG CCAGGAGACT CCACCGCCTT CTCAGCAGGC CCCACCGTTC 2401 AGCCTGACCC AGCCCCTGAG CCCCGTCCTG GAGCCTTCCT CCGAGCAGAT 2451 GCAATACAGC CCTTTCCTCA GCCAGTACCA AGAGATGCAG CTTCAGCCCC 2501 TGCCCTCCAC TTCCGGTCCC CGGGCTGCTC CTCCTCTGCC CACGCAGCTA 2551 CAGCAGCAGC AGCCGCCACC GCCACCACCC CCTCCACCAC CACGACAGCC 2601 AGGAGCTGCC CCAGCCCCCT TACAGTTCTC CTATCAGACT TGTGAGCTGC 2651 CAAGCGCTGC TTCCCCTGCG CCAGACTATC CCACTCCCTG TCAGTATCCT 2701 GTGGATGGAG CCCAGCAGAG CGACCTAACG GGGCCAGACT GTCCCAGAAG 2751 CCCAGGACTG CAAGAGGCCC CCTCCAGCTA CGACCCACTA GCCCTCTCTG 2801 AGCTACCTGG ACTCTTTGAT TGTGAAATGC TAGACGCTGT GGATCCACAA 2851 CACAACGGGT ATGTCCTGGT GAATTAGTCT CAGCACAGGA ATTGAGGTGG 2901 GTCAGGTGAA GGAAGAGTGT ATGTTCCTAT TTTTATTCCA GCCTTTTAAA 2951 TTTAAAGCTT ATTTTCTTGC CCTCTCCCTA ACGGGGAGAA ATCGAGCCAC 3001 CCAACTGGAA TCAGAGGGTC TGGCTGGGGT GGATGTTGCT TCCTCCTGGT 3051 TCTGCCCCAC CACAAAGTTT TCTGTGGCAA GTGCTGGAAC ATAGTTGTAG 3101 GCTGAGGCTC CTGCCCTTCG GTCGAGTGGA GCAAGCTCTC GAGGGCAGCA 3151 CTGACAAATG TGTTCCTAAG AAGACATTCA GACCCAGGTC TTATGCAGGA 3201 TTACATCCGT TTATTATCAA GGGCAACCTT GGTGAAAGCA GAAAGGGTGT 3251 GTGCTATTGC ATATATATGG GGGAAAAGGC AATATATTTT TCACTGAAGC 3301 TGAGCAACCA CATATTGCTA CAAGGCAAAT CAAGAAGACA TCAGGAAATC 3351 AGATGCACAG GAAATAAAGG AAAGCTGTGC TTTGTCATTG AATCCTAAGT 3401 TCTTAGCTGC TGATGCAAGT TGTCCCCCAA GGCCATCACA AAGCAGTGGG 3451 GCATGAGCTG TGTTTCAGGG GCCACTAAAT AACAGCTGGT ACTGACCCCA 3501 GAAACCGCCT TCATCTCCAT TCGGAAGCAG GTGACACACC CCTTCAGAAG 3551 GTGCCCTGGG TTGCCGAGTG TCAGAATATA CTCAGGACTC CAGAGGTGTC 3601 ACACGTGGAA CTGACAGGAG ACCCGCCACC GTGGAGGCAG GGGGCAAGAA 3651 ACTCAAGAAC GCATCAAGAG CACCAGCCCT GGGCCAGGGA AGACAGGCTC 3701 TTCCTGCAGT TTCTCGTGGA CACTGCTGGC TTGCGGGCAG TCGGTCTCCA 3751 GGGTACCTGT TGTCTCTTTT CCGATGTAAT AACTACTTTG ACCTTACACT 3801 ATATGTTGCT AGTAGTTTAT TGAGCTTTGT ATATTTGGAC AGTTTCATAT 3851 AGGGCTTAGA GATTTTAAGG ACATGATAAA TGAACTTTTC TGTCCCATGT 3901 GAAGTGGTAG TGCGGTGCCT TTCCCCCAGA TCATGCTTTA ATTCTTTCTT 3951 TTCTGTAGAA ACCAACAGTT TCCATTTATG TCAATGCTAA ATCCAAAGTC 4001 ACTTCAGAGT TTGTTTTCCA CCATGTGGGA ATCAGCATTC TTAATTTCGT 4051 TAAAGTTTTG ACTTGTAATG AAATGTTCAA GTATTACAGC AATATTCAAA 4101 GAAAGAACCA CAGATGTGTT AACCATTTAA GCAGATCATC TGCCAAACAT 4151 TATATTACTA ATAAAACTTA ACCAACACTT ACAATTCAGT CATCAAAGTA 4201 AGTAAAAATT AGATGCTACA GCTAGCTAAC TGTATCCCTA GAAATGATGA 4251 ATAATTTGCC ATTTGGACAG TTAACATCCA GGTGTTACAA AGTCAGTGTT 4301 AATTCTAAAG ATGATCATTT CTGCCCTTTA GAATGGCTTG TCCCATCAGC 4351 AGATGAATGT GTTAAGCACA AAGCATCTTC CTTAAAGCAC AAAGAGAGGG 4401 ACTAACTGAT GCTGCATCTA GAAAACACCT TTAAGTTGCC TTTCCTCTTT 4451 GTAGTTAGCG TTCAGGCAGG TGACGTGTGG AAAGTCTAGG GGGTTCCATT 4501 CTGGCCATGC GAGCCCAGCT CCTACCAACG TCGGTAACTT GAGCAGTCCC 4551 TGTTGCTGGC CAGAGACTGC CTGGTCGCCA GCGCTCACCA TGGGTGCCAG 4601 GATGCTTCGC AGAGGCACTG TGCTCACGGT TGGACTTGGT GTCAGTGGGA 4651 AAGGGCAGTG TGGGGACTGT CATTTTTGTG ATTTAATAAC ACACAGTGAA 4701 AATCCAGGAA GAATGAATTA AGCTTCTTCT GGGAGTTGTT TATTCCTGCT 4751 CGTGCTTAAG ATTGATGATT TCGTGAAATA AAGAACATCA TTTCATTTAA 4801 AAAAAAAAAA AAAAAAAGGG CGGCCGCTCT AGAGGATCCA AGCTTACGTA 4851 CGCGTGAAAA AAAAAAAG BLAST Results Entry HSG4921 from database EMBL: human STS SHGC-37164. Score = 1605, P = 1.9e-66, identities = 349/369 Entry AB018324 from database EMBL: Homo sapiens mRNA for KIAA0781 protein, partial eds. Score = 10725, P = O.Oe+00, identities = 2145/2145 Medline entries No Medline entry Peptide information for frame 1 ORF from the beginning to 2874 bp; peptide length: 959 Category: known protein 1 EQAERPSPKP SRAANPPARP RSCPPCLAAG PSMVMADGPR HLQRGPVRVG 51 FYDIEGTLGK GNFAVVKLGR HRITKTEVAI KIIDKSQLDA VNLEKIYREV 101 QIMKMLDHPH IIKLYQVMET KSMLYLVTEY AKNGEIFDYL ANHGRLNESE 151 ARRKFWQILS AVDYCHGRKI VHRDLKAENL LLDNNMNIKI ADFGFGNFFK 201 SGELLATWCG SPPYAAPEVF EGQQYEGPQL DIWSMGVVLY VLVCGALPFD 251 GPTLPILRQR VLEGRFRIPY FMSEDCEHLI RRMLVLDPSK RLTIAQIKEH 301 KWMLIEVPVQ RPVLYPQEQE NEPSIGEFNE QVLRLMHSLG IDQQKTIESL 351 QNKSYNHFAA IYFLLVERLK SHRSSFPVEQ RLDGRQRRPS TIAEQTVAKA 401 QTVGLPVTMH SPNMRLLRSA LLPQASNVEA FSFPASGCQA EAAFMEEECV 451 DTPKVNGCLL DPVPPVLVRK GCQSLPSNMM ETSIDEGLET EGEAEEDPAH 501 AFEAFQSTRS GQRRHTLSEV TNQLVVMPGA GKIFSMNDSP SLDSVDSEYD 551 MGSVQRDLNF LEDNPSLKDI MLANQPSPRM TSPFISLRPT NPAMQALSSQ 601 KREVHNRΞPV SFREGRRASD TSLTQGIVAF RQHLQNLART KGILELNKVQ 651 LLYEQIGPEA DPNLAPAAPQ LQDLASSCPQ EEVSQQQESV STLPASVHPQ 701 LSPRQSLETQ YLQHRLQKPS LLSKAQNTCQ LYCKEPPRSL EQQLQEHRLQ 751 QKRLFLQKQS QLQAYFNQMQ IAESSYPQPΞ QQLPLPRQET PPPSQQAPPF 801 SLTQPLSPVL EPSSEQMQYS PFLSQYQEMQ LQPLPSTSGP RAAPPLPTQL 851 QQQQPPPPPP PPPPRQPGAA PAPLQFSYQT CELPSAASPA PDYPTPCQYP 901 VDGAQQSDLT GPDCPRSPGL QEAPSSYDPL ALSELPGLFD CEMLDAVDPQ 951 HNGYVLVN BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_15kll, frame 1 No Alert BLASTP hits found Pedant information for DKFZphtes3_15kll, frame 1 Report for DKFZphtes3_15kll .1 [LENGTH] 926 [MW] 103915.77 [pi] 5.70 [HOMOL] TREMBL:AB018324_1 gene: "KIAA0781"; product: "KIAA0781 protein"; Homo sapiens mRNA for KIAA0781 protein, partial eds. 0.0 [FUNCAT] 01.05.04 regulation of carbohydrate utilization [S. cerevisiae, YDR477w] 8e-76 [FUNCAT] 11.01 stress response [S. cerevisiae, YDR477w] 8e-76 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YDR477w] 8e-76 [FUNCAT] 98 classification not yet clear-cut [S. cerevisiae, YCL024w] 4e-58 [FUNCAT] 03.25 cytokinesis [S. cerevisiae, YDR507c] 3e-56 [FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YDR507c] 3e-56 [FUNCAT] 30.02 organization of plasma membrane [S. cerevisiae, YDR122w] le-53 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YKLlOlw] 3e-53 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YKLlOlw] 3e-53 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YPL141c] 5e-51 [FUNCAT] 03.19 recombination and dna repair [S. cerevisiae, YPL153c] 3e-42 [FUNCAT] 03.22.01 cell cycle check point proteins [S. cerevisiae, YPL153c] 3e-42 [FUNCAT] 10.99 other signal-transduction activities [S. cerevisiae, YPL153c] 3e-42 [FUNCAT] 11.04 dna repair (direct repair, base excision repair and nucleotide excision repair) [S. cerevisiae, YPL153c] 3e-42 [FUNCAT] 03.01 cell growth [S. cerevisiae, YFR014c] 5e-42 [FUNCAT] 03.16 dna synthesis and replication [S. cerevisiae, YMROOlc] 2e-34 [FUNCAT] 03.10 sporulation and germination [S. cerevisiae, YGL180w] le-27 [FUNCAT] 08.13 vacuolar transport [S. cerevisiae, YGL180w] le-27 [FUNCAT] 06.13.04 lysosomal and vacuolar degradation [S. cerevisiae, YGL180w] le-27 [FUNCAT] 10.02.11 key kinases [S. cerevisiae, YBL105c] 3e-26 [FUNCAT] 04.99 other transcription activities [S. cerevisiae, YER129w] 3e-26 [FUNCAT] 02.19 metabolism of energy reserves (glycogen, trehalose) [S. cerevisiae, YPL031C] le-23 [ FUNCAT] 01 04.04 regulation of phosphate utilization [S. cerevisiae, YPL031c] le-23 [FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YPL031C] le-23 [FUNCAT] 03.13 meiosis [S. cerevisiae, YOR351c] 2e-23 [FUNCAT] 10.05.11 key kinases [S. cerevisiae, YHL007c] 8e-21 [ FUNCAT] 03.07 pheromone response, mating-type determination, sex-specific proteins [S. cerevisiae, YHL007c] 8e-21 [FUNCAT] 09.01 biogenesis of cell wall [S. cerevisiae, YPL140c] 2e-20 [FUNCAT] 10.03.11 key kinases [S. cerevisiae, YLR113w] le-20 [FUNCAT] 04.05.01.01 general transcription activities [S. cerevisiae, YDL108w] 3e-19 [FUNCAT] 10.05.09 regulation of g-protem activity [S. cerevisiae, YBLOlδw] 2e-18 [FUNCAT] 10.04.11 key kinases [S. cerevisiae, YLR362w] 3e-18 [FUNCAT] 04.03.99 other trna-transcription activities [S. cerevisiae, YOR061w] 4e-18 [FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylation, farnesylation and processing) [S. cerevisiae, YFL033c] 4e-17 [FUNCAT] 05.07 translational control [S. cerevisiae, YDR283c] 2e-16 [FUNCAT] 01.02.04 regulation of nitrogen and sulphur utilization [S. cerevisiae, YNL183c] 2e-14 [FUNCAT] 08.99 other intracellular-transport activities [S. cerevisiae, YNL183c] 2e-14 [FUNCAT] 09.04 biogenesis of cytoskeleton [S. cerevisiae, YNL020C] 5e-14 [FUNCAT] c energy conversion [M. gemtalium, MG109] 2e-12 [FUNCAT] 30.09 organization of intracellular transport vesicles [S. cerevisiae, YBR097w) le -10 [FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YBR097w] le-10 [FUNCAT] 30.08 organization of golgi [S. cerevisiae, YBR097w] le-10 [FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YBR097w] le-10 [FUNCAT] 10.04.99 other nutritional-response activities [S. cerevisiae, YJR059w] 4e-09 [FUNCAT] 01.06.10 regulation of lipid, fatty-acid and sterol biosynthesis [S. cerevisiae YHR079c] le-07 [FUNCAT] 30.07 organization of endoplasmatic reticulum [S. cerevisiae, YHR079c] le-07 [FUNCAT] 08.19 cellular import [S. cerevisiae, YNL154c] 2e-04 [BLOCKS] BL00415A Synapsins proteins [BLOCKS] BL00239B Receptor tyrosine kinase class II proteins [BLOCKS] BL00107A Protein kinases ATP-binding region proteins [SCOP] dlgol 1.1.1. MAP kinase Erk2 [rat Rattus norvegicus 3e-78 [SCOP] dlwfc 1.1.1.8 MAP kinase p38 [human (Homo sapiens) le-81 [SCOP] dlkoa_2 1.1.1.7 (1-350) Twitchm, kinase domain [Caenorhabditi 5e-89 [SCOP] dlkoba_ 1.1. .1.6 Twitchm, kinase domain [California sea har 5e-86 [SCOP] dlphk 1.1..1.5 gamma-subumt of glycogen phosphorylase kinas 3e-80 [SCOP] dlirk 1.1.2.4 insulin receptor [Human (Homo sapiens) 6e-70 [SCOP] dlapme_ 1.1.1.4 cAMP-dependent PK, catalytic subunit [mouse (Mu le-95 [SCOP] dlfgka_ 1.1.2.3 Fibroblast growth factor receptor 1 [human (Hom 7e-71 [SCOP] dlydse_ 1.1.1.3 cAMP-dependent PK, catalytic subunit [bovine (Bo 2e-96 [SCOP] dlfmk_3 1.1.2.2 (168-437) c-src tyrosine kinase [human (Hom 2e-72 [SCOP] dlcdka_ 1.1.1.2 cAMP-dependent PK, catalytic subunit [pig (Su 5e-97 [SCOP] d2hckb3 1.1.2.1 (167-437) Haemopoetic cell kinase Hck [huma 2e-68 [SCOP] dlcsn 1.1.1.11 Casein kmase-1, CK1 [Schizosaccharomyces pombe 3e-53 [SCOP] dl sua_ 1.1.1.1 Cyclin-dependent PK [Human (Homo sapiens) 3e-78 [SCOP] dlckιa__ 1.1.1.10 Casein kmase-1, CKl [rat (Rattus norvegicus) le-58 [EC] 2.7.1.117 Myosin-l ght-chain kinase 3e-49 [EC] 2. 7.1.109 [Hydroxymethylglutaryl-CoA reductase (NADPH) ] kinase 4e-78 [EC] 2 7.1.38 Phosphorylase kinase 3e-41 [EC] 2 7.1.37 Protein kinase 7e-45 [EC] 2 7.1.123 Ca2+/calmodulιn-dependent protein kinase 5e-42 [EC] 2 7.1.128 [Acetyl-CoA carboxylase] kinase 4e-78 [PIRKW] phosphotransferase 3e-93 [PIRKW] nucleus 2e-74 ' [PIRKW] calcium 2e-40 [PIRKW] transferase 3e-33 [PIRKW] duplication 2e-32 [PIRKW] tandem repeat 7e-45 [PIRKW] phorbol ester binding 4e-33 [PIRKW] zinc 4e-33 [PIRKW] ion transport le-32 [PIRKW] cell cycle control le-45 [PIRKW] seπne/threonme-specific protein kmase 2e-97 [PIRKW] oncogene le-34 [PIRKW] phospholipid binding 2e-32 [PIRKW] autophosphorylation 2e-74 [PIRKW] brain 6e-36 [PIRKW] heterotetramer 8e-38 [PIRKW] mitosis le-45 [PIRKW] polymer 5e-41 [PIRKW] magnesium 6e-80 [PIRKW] ATP 2e-97 [PIRKW] polyprotein le-34 [PIRKW] alternative initiators 2e-31 [PIRKW] phosphoprotein 2e-74 [PIRKW] apoptosis 8e-38 [PIRKW] cGMP binding 4e-33 [PIRKW] glycoprotein 3e-36 [PIRKW] skeletal muscle 8e-38 [PIRKW] protein kinase 2e-50 [PIRKW] testis 5e-41 [PIRKW] cAMP binding 8e-38 [PIRKW] transforming protein 4e-33 [PIRKW] purme nucleotide binding 7e-52 [PIRKW] calcium binding 7e-45 [PIRKW] alternative splicing 5e-42 [PIRKW] P-loop 7e-52 [PIRKW] lipoprotein 8e-38 [PIRKW] proto-oncogene 4e-33 [PIRKW] segmentation le-34 [PIRKW] core protein le-34 [PIRKW] muscle 8e-38 [PIRKW] myristylation 8e-38 [PIRKW] EF hand 7e-45 [PIRKW] cell division 3e-49 [PIRKW] homodimer le-32 [PIRKW] calmodulin binding 5e-42 [SUPFAM] ribosomal protein S6 kinase II le-34 [SUPFAM] calcium-dependent protein kinase 7e-45 [SUPFAM] AMP-activated protein kinase 6e-80 [SUPFAM] protein kmase akt 3e-36 [SUPFAM] protein kinase SPK1 7e-41 [SUPFAM] unassigned Ser/Thr or Tyr-specific protein kinases 8e-99 [SUPFAM] Ca2+/calmodulιn-dependent protein kinase 5e-42 [SUPFAM] calmodulin repeat homology 7e-45 [SUPFAM] cAMP receptor protein cyclic nucleotide-bmding domain homology 3e-33 [SUPFAM] protein kmase DUN1 6e-36 [SUPFAM] protein kinase C zeta 4e-33 [SUPFAM] Dictyostelium cAMP-dependent protein kinase catalytic chain 2e-34 [SUPFAM] death-associated protein kinase 8e-38 [SUPFAM] pleckstrm repeat homology 3e-36 [SUPFAM] ankyrin repeat homology 8e-38 [SUPFAM] protein kinase homology 8e-99 [SUPFAM] Ca2+/calmodulιn-dependent protein kinase II 6e-38 [SUPFAM] protein kinase C zinc-binding repeat homology 4e-33 [SUPFAM] protein kinase C delta 2e-32 [SUPFAM] cGMP-dependent protein kinase 3e-33 [SUPFAM] protein kinase cdrl le-45 [SUPFAM] kinase-related transforming protein 2e-50 [SUPFAM] Ca2+/calmodulιn-dependent protein kinase I 8e-42 [SUPFAM] kinase interaction domain homology 7e-41 [SUPFAM] gag-akt polyprotein le-34 [PROSITE] PROTEIN_KINASE_ATP 1 [PROSITE] MYRISTYL 3 [PROSITE] AMIDATION 2 [PROSITE] CAMP_PHOSPHO_SITE 4 [PROSITE] CK2_PHOSPHO_SITE 15 [PROSITE] TYR_PHOSPHO_SITE 2 [PROSITE] PKC_PHOSPHO_SITE 10 [PROSITE] ASN_GLYCOSYLATION 2 [PROSITE] PROTEIN_KINASE_ST 1 [PFAM] Eukaryotic protein kinase domain [KW] Irregular [KW] 3D [KW] LOW COMPLEXITY 12.31 % SEQ MVMADGPRHLQRGPVRVGFYDIEGTLGKGNFAVVKLGRHRITKTEVAIKIIDKSQLDAVN SEG IctpE EEECTTTEEEEEEEETTTTEEEEEEEEEHHHHHHHC SEQ LEKIYREVQIMKMLDHPHIIKLYQVMETKSMLYLVTEYAKNGEIFDYLANHGRLNESEAR SEG IctpE HHHHHHHHHHHHCCCTTTBCCEEEEEEETTEEEEEEECTTTTBHHHHHHHHCCCCHHHHH SEQ RKFWQILSAVDYCHGRKIVHRDLKAENLLLDNNMNIKIADFGFGNFFKSGELLATWCGSP SEG IctpE HHHHHHHHHHHHHHHCCEECCCCCGGGEEETTTTCEEECCTTTTEETT-TTBC-CCCCCG SEQ PYAAPEVFEGQQYEGPQLDIWSMGVVLYVLVCGALPFDGPTLPILRQRVLEGRFRIPYFM SEG IctpE GGCCHHHHHCCCBC-HHHHHHHHHHHHHHHHHCCTTTTTTTHHHHHHHHHHCCCCCTTTT SEQ SEDCEHLIRRMLVLDPSKRLTIAQIKEHKWMLIEVPVQRPVLYPQEQENEPSIGEFNEQV SEG IctpE CHHHHHHHHHTTTTTGGGTTTHHHHHHCGG SEQ LRLMHSLGIDQQKTIESLQNKSYNHFAAIYFLLVERLKSHRSSFPVEQRLDGRQRRPSTI SEG IctpE SEQ AEQTVAKAQTVGLPVTMHSPNMRLLRSALLPQASNVEAFSFPASGCQAEAAFMEEECVDT SEG IctpE SEQ PKVNGCLLDPVPPVLVRKGCQSLPSNMMETSIDEGLETEGEAEEDPAHAFEAFQSTRSGQ SEG xxxxxxxxxxx IctpE SEQ RRHTLSEVTNQLVVMPGAGKIFSMNDSPSLDSVDSEYDMGSVQRDLNFLEDNPSLKDIML SEG IctpE ON O HMM *YeιgRιIGeGsFGtVYkCιWr .TGelVAIKIIkkrsms F1REI Y I++++G+G+F++V+++++R T +VAIKII+K++++ + RE+ Query 20 YDIEGTLGKGNFAVVKLGRHRITKTEVAIKIIDKSQLDAVNLEKIYREV 68 HMM qlMRrLnHPNIIRFYDwFedddDHIYMIMEYMeGGDLFDYIrrngpMsEw QIM++L+HP+II++Y ++E +++ +Y+++EY+ +G++FDY+ ++G+++E Query 69 QIMKMLDHPHIIKLYQVME-TKSMLYLVTEYAKNGEIFDYLANHGRLNES 117 HMM elrflMyQILrGMeYLHSMgllHRDLKPENILIDeNgqlKIcDFGLARqM E+R+ ++QIL++++Y+H ++I+HRDLK+EN+L+D+N++IKI+DFG+ ++ Query 118 EARRKFWQILSAVDYCHGRKIVHRDLKAENLLLDNNMNIKIADFGFGNFF 167 HMM nnYerMttfCGTPWYMMAPEVIImg.nyYttkVDMWSFGCILWEMMTGep +++E++ T CG+P+Y APEV +G +Y +++ D+WS+G++L+ +++G + Query 168 KSGELLATWCGSPPYA-APEV-FEGQQYEGPQLDIWSMGVVLYVLVCGAL 215 HMM PFyddnMemlmrliqrfrrpfWpnCSeElyDFMrwCWnyDPekRPTFrQI PF++ ++ + + +++ R+++++ +SE++ +++R+++ +DP+KR+T+ QI Query 216 PFDGPTLPILRQRVLEGRFRIPYFMSEDCEHLIRRMLVLDPSKRLTIAQI 265 HMM LnHPWF* +H W+ Query 266 KEHKWM 271 DKFZphtes3_17flO group: testes derived DKFZphtes3_15jl8 encodes a novel 710 ammo acid protein with weak similarity to neurofilament proteins . No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . similarity to neurofilament proteins Sequenced by GBF Locus : unknown Insert length: 2533 bp Poly A stretch at pos. 2507, no polyadenylation signal found 1 CTTCAGTTCA ACTAAAAATG GACAGATCTC AGCAGACCAG CCGTACAGGA 51 TACTGGACCA TGATGAACAT CCCCCCTGTA GAAAAAGTGG ACAAGGAACA 101 ACAGACATAC TTTAGTGAAT CAGAAATAGT GGTTATTTCC AGGCCAGATA 151 GTTCTTCTAC AAAGTCAAAG GAAGATGCCC TGAAACATAA ATCGTCGGGA 201 AAGATTTTTG CTAGTGAACA CCCTGAATTT CAACCAGCAA CAAACAGCAA 251 TGAAGAAATT GGGCAGAAAA ATATCAGCAG AACTTCATTT ACTCAGGAGA 301 CTAAAAAAGG TCCCCCAGTA CTTTTAGAAG ATGAGCTTAG GGAAGAAGTA 351 ACTGTACCTG TTGTACAAGA AGGTTCTGCT GTTAAAAAAG TGGCTTCTGC 401 TGAAATAGAG CCTCCATCAA CAGAAAAATT CCCAGCTAAA ATACAGCCTC 451 CATTAGTTGA AGAGGCCACT GCTAAAGCGG AGCCCAGACC TGCTGAAGAG 501 ACCCATGTCC AAGTACAGCC ATCAACTGAA GAGACTCCTG ATGCTGAGGC 551 AGCCACTGCA GTTGCGGAGA ATTCTGTTAA AGTTCAGCCT CCACCTGCTG 601 AAGAGGCCCC TTTAGTGGAG TTTCCTGCTG AAATTCAGCC TCCATCAGCT 651 GAAGAGTCTC CTTCTGTAGA GCTTCTGGCT GAAATTCTGC CTCCATCAGC 701 TGAAGAGTCC CCTTCAGAAG AGCCTCCTGC TGAAATTCTG CCTCCACCAG 751 CTGAAAAGTC TCCTTCAGTA GAGCTTCTTG GTGAAATTCG GTCTCCCTCA 801 GCACAAAAGG CTCCCATTGA AGTACAGCCT TTACCAGCTG AGGGCGCCCT 851 TGAAGAGGCC CCAGCTAAAG TAGAGCCTCC CACTGTTGAA GAGACCCTTG 901 CTGAAGTTCA GCCTCTATTA CCTGAAGAGG CTCCTAGAGA AGAGGCTCGA 951 GAACTTCAGC TTTCAACAGC TATGGAGACC CCTGCAGAAG AGGCTCCTAC 1001 TGAATTTCAG TCTCCATTAC CTAAAGAGAC CACTGCAGAA GAGGCCTCTG 1051 CTGAAATTCA GCTTCTAGCA GCTACGGAGC CTCCTGCAGA TGAAACTCCT 1101 GCCGAAGCTC GGTCTCCACT ATCTGAGGAG ACTTCTGCAG AAGAGGCTCA 1151 TGCTGAAGTT CAATCTCCAT TAGCTGAAGA GACCACTGCA GAAGAGGCCT 1201 CTGCTGAAAT TCAGCTTCTA GCAGCTATAG AGGCTCCTGC AGATGAAACT 1251 CCTGCTGAAG CTCAGTCTCC ACTATCTGAG GAGACTTCTG CAGAAGAGGC 1301 TCCTGCTGAA GTTCAGTCTC CATCAGCTAA GGGAGTTTCT ATAGAAGAGG 1351 CCCCTCTTGA GCTTCAGCCT CCATCAGGTG AAGAGACCAC TGCAGAAGAG 1401 GCCTCTGCTG CAATTCAGCT TCTAGCAGCT ACAGAGGCTT CTGCAGAAGA 1451 GGCTCCTGCT GAAGTTCAGC CTCCACCAGC TGAGGAGGCC CCCGCTGAAG 1501 TTCAGCCTCC ACCAGCTGAG GAGGCCCCCG CTGAAGTTCA GCCTCCACCA 1551 GCTGAGGAGG CCCCCGCTGA AGTTCAGCCT CCACCAGCTG AGGAGGCCCC 1601 CGCTGAAGTT CAGCCTCCAC CAGCTGAGGA GGCCCCCGCT GAAGTTCAGC 1651 CTCCACCAGC TGAGGAGGCC CCCTCTGAAG TTCAGCCTCC ACCAGCTGAG 1701 GAGGCCCCTG CTGAAGTTCA GTCTCTACCA GCTGAGGAGA CTCCTATAGA 1751 AGAGACCCTT GCTGCAGTAC ACTCTCCCCC AGCTGATGAT GTCCCTGCAG 1801 AAGAGGCCTC CGTTGACAAA CATTCCCCAC CAGCTGATTT GCTTCTGACT 1851 GAGGAGTTTC CTATAGGAGA GGCCTCTGCT GAAGTTTCAC CTCCACCATC 1901 TGAACAAACC CCTGAAGATG AGGCTCTGGT AGAGAATGTG TCTACAGAAT 1951 TTCAGTCACC GCAGGTGGCA GGAATTCCAG CAGTAAAATT AGGATCGGTT 2001 GTTTTGGAAG GTGAAGCAAA ATTTGAAGAG GTTTCAAAAA TCAATTCTGT 2051 CCTTAAAGAT TTGTCTAATA CCAATGATGG ACAGGCTCCC ACTCTTGAAA 2101 TAGAAAGTGT TTTTCATATA GAATTAAAAC AACGTCCTCC TGAACTGTAG 2151 TCAGGTTGTA CCTAAGCTAG CAATCAGAAG CTACATGGTT TTGGAAGAAC 2201 ATACTTTAGA AAAGGGTGGG CAGCAGGAAG TAGCTTTGTC AATAAGGCAA 2251 ATTAAAGGGG ACCCCAAGAC TTGGAATACA GGTTGGAAAA TGAACAATAA 2301 AAACTGTAGC AGCATAAAAT TACTTGTGTT AATTTCATTC AAATTTATGG 2351 CATGAAAAAT ACCTATTTTG AAAGTAAGTT TATAATTGAA AAAAATTGCT 2401 TAAAATATCC TTCCTACAGT AAACTTGTTG ACACGAGTAA AGTTTAATCT 2451 GCAGCCATCT TTTCTTGTCT TTGCCTTCCC TTTATAAGTA AATATAGTTT 2501 CTAGTGGAAA AAAAAAAAAA AAAAAAAAAA AAA BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 3 ORF from 18 bp to 2147 bp; peptide length: 710 Category: similarity to known protein Classification: unclassified 1 MDRSQQTSRT GYWTMMNIPP VEKVDKEQQT YFSESEIVVI SRPDSSSTKS 51 KEDALKHKSS GKIFASEHPE FQPATNSNEE IGQKNISRTS FTQETKKGPP 101 VLLEDELREE VTVPVVQEGS AVKKVASAEI EPPSTEKFPA KIQPPLVEEA 151 TAKAEPRPAE ETHVQVQPST EETPDAEAAT AVAENSVKVQ PPPAEEAPLV 201 EFPAEIQPPS AEESPSVELL AEILPPSAEE SPSEEPPAEI LPPPAEKSPS 251 VELLGEIRSP SAQKAPIEVQ PLPAEGALEE APAKVEPPTV EETLAEVQPL 301 LPEEAPREEA RELQLSTAME TPAEEAPTEF QΞPLPKETTA EEASAEIQLL 351 AATEPPADET PAEARSPLSE ETSAEEAHAE VQSPLAEETT AEEASAEIQL 401 LAAIEAPADE TPAEAQSPLS EETSAEEAPA EVQSPSAKGV SIEEAPLELQ 451 PPSGEETTAE EASAAIQLLA ATEASAEEAP AEVQPPPAEE APAEVQPPPA 501 EEAPAEVQPP PAEEAPAEVQ PPPAEEAPAE VQPPPAEEAP AEVQPPPAEE 551 APSEVQPPPA EEAPAEVQSL PAEETPIEET LAAVHSPPAD DVPAEEASVD 601 KHSPPADLLL TEEFPIGEAS AEVSPPPSEQ TPEDEALVEN VSTEFQSPQV 651 AGIPAVKLGΞ VVLEGEAKFE EVSKINSVLK DLSNTNDGQA PTLEIESVFH 701 IELKQRPPEL BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_17f10, frame 3 PIR:A37221 neurofilament triplet H protein - rat, N = 1, Score = 480, P = 7.4e-43 TREMBL :RNNFLH_1 Rat heavy neurofilament subunit (NF-H) mRNA, 3' end., N = 1, Score = 475, P = le-42 >PIR:A37221 neurofilament triplet H protein - rat Length = 1,072 HSPs: Score = 480 (72.0 bits), Expect = 7.4e-43, P = 7.4e-43 Identities = 185/622 (29%), Positives = 320/622 (51%) Query: 33 SESEIVVISRPDSSSTKSKEDALKHKSSGKIFASEHPEFQPATNSNEEIGQKNISRTSFT 92 SE +1 V+ + + + +E + + + ++ E E Q E G + + TS Sbjct: 436 SEEKIKVVEKSEKETVIVEEQTEEIQVTEEVTEEEDKEAQGEEEEEAEEGGEEAATTSPP 495 Query: 93 QETKKGPPVLLEDELREEVTVPVVQEGSAVKKVASAEIEPPSTEKFPAKIQPPLVEEATA 152 E P + ++EE P + A K + AE + P+ K PA+++ P ++ A Sbjct: 496 AEEAASPEKETKSPVKEEAKSPAEAKSPAEAK-SPAEAKSPAEVKSPAEVKSPAEAKSPA 554 Query: 153 KAEPRPAEETHVQVQPSTEETPDAEAATAVAENSVKVQPPPAEEAP-LVEFPAEIQPPSA 211 +A+ PAE V+ P+T ++P + A A++ +V+ P ++P + PAE + P+ Sbjct: 555 EAKS-PAE VK-SPATVKSPAEAKSPAEAKSPAEVKSPATVKSPGEAKSPAEAKSPAE 609 Query: 212 EESP-SVELLAEILPPSAEESPSE-EPPAEILPPPAEKSPS-VELLGEIRSPSAQKAPIE 268 +SP + AE P++ +SP E + PAE P KSP+ V+ E +SP+ K+P+ Sbjct: 610 VKSPVEAKSPAEAKSPAΞVKSPGEAKSPAEAKSPAEVKSPATVKSPVEAKSPAEVKSPVT 669 Query: 269 VQPLPAEGALEEAPAKVEPPTVEETLAEVQPLLPEEAPREEARELQLSTAMETPAE-EAP 327 V+ PAE ++P +V+ P ++ +E + ++P E A+ ++PAE ++P Sbjct: 670 VKS-PAEA KSPVEVKSPASVKSPSEAKSPAGAKSPAE-AKS PVVAKSPAEAKSP 721 Query: 328 TEFQSPLPKETTAEEASAEIQLLAATEPPAD-ETPAEARSPLSEETSAEEAHAEVQS 383 E + P ++ AE S A + PA+ ++PAEA+SP+ E S E+A + V+ Sbjct: 722 AEAKPPAEAKSPAEAKSP AEAKSPAEAKSPAEAKSPV-EVKSPEKAKSPVKEGAK 775 Query: 384 PLAEETTAEEASAEIQLLAAIEAPAD-ETPAEAQSPLSEET-SAEEAPA-EVQSPSAKGV 440 LAE + E+A + ++ 1+ PA+ ++P +A+SP+ EE S E+A +V+SP AK Sbjct: 776 SLAEAKSPEKAKSPVK—EEIKPPAEVKSPEKAKSPMKEEAKSPEKAKTLDVKSPEAKTP 833 Query: 441 SIEEA—PLELQPPSGEETTA-EEASAAIQLLAATEAΞA EEAPAEVQPPPAEEAPAE 494 + EEA P +++ P ++ A EEA + + TE A EE + V+ A+E P + Sbjct: 834 AKEEAKRPADIRSPEQVKΞPAKEEAKSPEKEETRTEKVAPKKEEVKSPVEEVKAKEPPKK 893 Query: 495 VQPPPAEEAP-AEVQPPPAEEAPAEVQPPPAEEAPAEVQPPPAEEAPAEVQPPPAEEAPS 553 V+ P EV+ +EAP E Q P AEE + P +++P E + EEA Sbjct: 894 VEEEKTPATPKTEVKESKKDEAPKEAQKPKAEEKEPLTEKP—KDSPGEAKK EEAKE 948 Query: 554 EVQPPPAEEAPAEV QSLP AEETPIEETL—AAVHSPPADDVPAEEASVD-KHS 603 + P EE PA++ ++ P AE+ +E + P ++VPA D K Sbjct: 949 KKAAAPEEETPAKLGVKEEAKPKEKAEDAKAKEPSKPSEKEKPKKEEVPAAPEKKDTKEE 1008 Query: 604 PPADLLLTEEFPIGEASAEVSPP—PSEQT-PEDEALVENVSTEFQSPQ 649 + EE P +A A+ P E + P+ E ++ ST+ + Q Sbjct: 1009 KTTESKKPEEKPKMQAKAKEEDKGLPQEPSKPKTEKAEKSSSTDQKDSQ 1057 Score = 473 (71.0 bits), Expect = 4.8e-42, P = 4.8e-42 Identities = 184/628 (29%), Positives = 310/628 (49%) Query: 18 IPPVEKVDKEQQTYFSESEIVVISRP DSSSTKSKEDALKHKSSGKIFASEHPEFQPA 74 I VEK +KE ++E + ++ + E+ + + G+ A+ P + A Sbjct: 440 IKVVEKSEKETVIVEEQTEEIQVTEEVTEEEDKEAQGEEEEEAEEGGEEAATTSPPAEEA 499 Query: 75 TNSNEEIGQKNISRTSFTQETKKGPPVLLEDELREEVTVPVVQEGSAVKKVASAEIEPPS 134 + +E + + + + K P E + E P + A K + AE + P+ Sbjct: 500 ASPEKET-KSPVKEEAKSPAEAKSPA EAKSPAEAKSPAEVKSPAEVK-SPAEAKSPA 554 Query: 135 TEKFPAKIQPPLVEEATAKAEPRPAEETHVQVQ-PΞTEETPDAEAATAVAENSVKVQPPP 193 K PA+++ P ++ A+A+ ++ +V+ P+T ++P + A A++ +V+ P Sbjct: 555 EAKSPAEVKSPATVKSPAEAKSPAEAKSPAEVKSPATVKSPGEAKSPAEAKSPAEVKSPV 614 Query: 194 AEEAPL-VEFPAEIQPPSAEESPS-VELLAEILPPSAEESPSE-EPPAEILPPPAEKSPS 250 ++P + PA ++ P +SP+ + AE+ P+ +SP E + PAE+ P KSP+ Sbjct: 615 EAKSPAEAKSPASVKSPGEAKSPAEAKSPAEVKSPATVKSPVEAKSPAEVKSPVTVKSPA 674 Query: 251 -VELLGEIRSPSAQKAPIEVQ-PLPAEGALE-EAPAKVEPPTVEETLAEVQPLLPEEAPR 307 + E++SP++ K+P E + P A+ E ++P + P ++ AE +P ++P Sbjct: 675 EAKSPVEVKSPASVKSPSEAKSPAGAKΞPAEAKSPVVAKSPAEAKSPAEAKPPAEAKSPA 734 Query: 308 EEARELQLSTAME--TPAE-EAPTEFQSP LP-KE TTAEEASAEIQLLAATE-- 354 E + + E +PAE ++P E +SP P KE + AE S E E Sbjct: 735 EAKSPAEAKSPAEAKSPAEAKSPVEVKSPEKAKSPVKEGAKSLAEAKSPEKAKSPVKEEI 794 Query: 355 -PPAD-ETPAEARSPLSEET-SAEEAHA-EVQSPLAEETTAEEAS—AEIQLLAAIEAPA 408 PPA+ ++P +A+SP+ EE S E+A +V+SP A+ EEA A+I+ +++PA Sbjct: 795 KPPAEVKSPEKAKSPMKEEAKSPEKAKTLDVKSPEAKTPAKEEAKRPADIRSPEQVKSPA 854 Query: 409 DETPAEAQSPLΞEETSAEE-APA--EVQSPSAKGVSIEEAPLELQPPSGEETTAEEASAA 465 E EA+SP EET E+ AP EV+SP +EE + +PP E EE + A Sbjct: 855 KE EAKSPEKEETRTEKVAPKKEEVKSP VEEVKAK-EPPKKVE EEKTPA 901 Query: 466 IQLLAATEASAEEAPAEVQPPPAEEAPAEVQPPPAEEAPAEVQPPPAEEAPAEVQPPPAE 525 E+ +EAP E Q P AEE + P +++P E + A+E A P E Sbjct: 902 TPKTEVKESKKDEAPKEAQKPKAEEKEPLTEKP--KDSPGEAKKEEAKEKKAAA PEE 956 Query: 526 EAPAEV QPPPAEEAPAEVQPPPAEEAPSEVQPPPAEEAPAEVQSLPAEETPIEETL 581 E PA++ + P E+A P++ PSE + P EE PA + +E E+ Sbjct: 957 ETPAKLGVKEEAKPKEKAEDAKAKEPSK—PSEKEKPKKEEVPAAPEKKDTKEEKTTESK 1014 Query: 582 AAVHSPPADDVPAEEASVDKHSPPADLL-LTEEFPIGEASAEVSPPPSEQTPEDEA 636 P EE DK P TE+ ++ + PSE+ PED+A Sbjct: 1015 KPEEKPKMQAKAKEE DKGLPQEPSKPKTEKAEKSSSTDQKDSQPSEKAPEDKA 1067 Score = 421 (63.2 bits), Expect = 3.7e-36, P = 3.7e-36 Identities = 162/540 (30%), Positives = 275/540 (50%) Query: 135 TEKFPAKIQPPLVEEATAKAEPR PAEETHVQVQPSTEETPDAEAATAVAENSVKV 189 TE P KI P + K+E + +E+ V V+ TEE E T E + Sbjct: 419 TEGLP-KI-PSMSTHIKVKSEEKIKVVEKSEKETVIVEEQTEEIQVTEEVTE—EEDKEA 474 Query: 190 QPPPAEEAPLVEFPAEIQPPSAEESPSVELLAEILPPSAEE—SPSE-EPPAEILPPPAE 246 Q EEA A P AEE+ S E E P EE SP+E + PAE P Sbjct: 475 QGEEEEEAEEGGEEAATTSPPAEEAASPE—KETKΞPVKEEAKSPAEAKSPAEAKSPAEA 532 Query: 247 KSPSVELLGEIRSPSAQKAPIEVQPLPAEGALEEAPAKVEPPTVEETLAEVQPLLPEEAP 306 KSP+ E++SP+ K+P E + PAE ++PA+V+ P ++ AE + ++P Sbjct: 533 KSPA EVKSPAEVKSPAEAKS-PAEA KSPAEVKSPATVKSPAEAKSPAEAKSP 583 Query: 307 REEARELQLSTAME—TPAE-EAPTEFQSPLPKETTAEEAS-AEIQLLAATEPPAD-ETP 361 E + + E +PAE ++P E +SP+ ++ AE S A ++ + PA+ ++P Sbjct: 584 AEVKSPATVKSPGEAKSPAEAKSPAEVKSPVEAKSPAEAKSPASVKSPGEAKΞPAEAKSP 643 Query: 362 AEARSPLSEETSAE-EAHAEVQΞPLAEETTAEEASAEIQLLAAIEAPAD-ETPAEAQSPL 419 AE +SP + ++ E ++ AEV+SP+ ++ AE A + ++ +++PA ++P+EA+SP Sbjct: 644 AEVKSPATVKSPVEAKSPAEVKSPVTVKSPAE-AKSPVE VKSPASVKSPSEAKSP- 697 Query: 420 SEETSAEEAPAEVQSPS-AKGVΞIEEAPLELQPPSGEETTAEEASAAIQLLAATEASAEE 478 + ++PAE +SP AK + ++P E +PP+ ++ AE S A A + A A+ Sbjct: 698 AGAKSPAEAKSPVVAKSPAEAKSPAEAKPPAEAKSPAEAKSPAE AKSPAEAK- 749 Query: 479 APAEVQPPPAEEAPAEVQPPPAEEAP—AEVQPPPAEEAPA--EVQPPPAEEAPAEVQPP 534 +PAE + P ++P + + P E A AE + P ++P E++PP ++P + + P Sbjct: 750 SPAEAKSPVEVKSPEKAKSPVKEGAKSLAEAKSPEKAKSPVKEEIKPPAEVKSPEKAKSP 809 Query: 535 PAEEAPAEVQPPPAEEAPSEVQPPPAEEA—PAEVQSLPAEETPIEETLAAVHSPPADDV 592 EEA + + + E + P EEA PA+++S ++P +E SP ++ Sbjct: 810 MKEEAKSPEKAKTLDVKSPEAKTPAKEEAKRPADIRSPEQVKSPAKEE AKSPEKEET 866 Query: 593 PAEEASVDKHS--PPADLLLTEEFPIGEASAEVSPPPSEQTPEDEALVENVSTEFQSPQV 650 E+ + K P + + +E P + E P + +T E+ + E Q P+ Sbjct: 867 RTEKVAPKKEEVKSPVEEVKAKEPP—KKVEEEKTPATPKTEVKESKKDEAPKEAQKPKA 924 Query: 651 AGIPAVKLGSVVLEGEAKFEEVSK 674 + GEAK EE + Sbjct: 925 EEKEPLTEKPKDSPGEAKKEEAKE 948 Score = 406 (60.9 bits), Expect = 1.7e-34, P = 1.7e-34 Identities = 123/390 (31%), Positives = 213/390 (54%) Query: 308 EEARELQLSTAMETPAEEAPTEFQSPLPKETTAEEASAEIQLLAATEPPADETPA EA 364 E+ E+Q++ E EE E Q +E AEE E A T PPA+E + E Sbjct: 455 EQTEEIQVT EEVTEEEDKEAQGE—EEEEAEEGGEEA ATTSPPAEEAASPEKET 506 Query: 365 RSPLSEETSAEEAHAEVQSPLAEETTAEEAS-AEIQLLAAIEAPAD-ETPAEAQSPLSEE 422 +SP+ EE + AE +SP ++ AE S AE++ A +++PA+ ++PAEA+SP + Sbjct: 507 KSPVKEEAKSP AEAKSPAEAKSPAEAKSPAEVKSPAEVKSPAEAKSPAEAKSPAEVK 563 Query: 423 TSAE-EAPAEVQSPS-AKGVSIEEAPLELQPPSGEETTAEEASAAIQLLAATEASAEEAP 480 + A ++PAE +SP+ AK + ++P ++ P GE + EA + ++ + EA ++P Sbjct: 564 SPATVKSPAEAKSPAEAKSPAEVKSPATVKSP-GEAKSPAEAKSPAEVKSPVEA KSP 619 Query: 481 AEVQPPPAEEAPAEVQPPPAEEAPAEVQPPPAEEAPAEVQPPPAEEAPAEVQPPPAEEAP 540 AE + P + ++P E + P ++PAEV+ P ++P E + P ++P V+ P ++P Sbjct: 620 AEAKSPASVKSPGEAKSPAEAKSPAEVKSPATVKSPVEAKSPAEVKΞPVTVKSPAEAKSP 679 Query: 541 AEVQPPPAEEAPSEVQPPPAEEAPAEVQSLPAEETPIEETLAAVHSPPAD-DVPAEEASV 599 EV+ P + ++PSE + P ++PAE +S ++P E A PPA+ PAE S Sbjct: 680 VEVKSPASVKSPSEAKSPAGAKSPAEAKSPVVAKSPAEAKSPAEAKPPAEAKSPAEAKSP 739 Query: 600 DKHSPPADLLLTEEFPIGEASAEVSPPPSEQTPEDEALVENVSTEFQSPQVAGIPAVKLG 659 + PA+ E ++ EV P ++P E ++++ E +SP+ A P VK Sbjct: 740 AEAKSPAEAKSPAE AKSPVEVKSPEKAKSPVKEG-AKSLA-EAKSPEKAKSP-VK-E 792 Query: 660 SVVLEGEAKFEEVSKINSVLKDLSNTNDGQAPTLEIES 697 + E K E +K S +K+ + + + +A TL+++S Sbjct: 793 EIKPPAEVKSPEKAK—ΞPMKEEAKSPE-KAKTLDVKS 827 Score = 255 (38.3 bits), Expect = 5.5e-18, P = 5.5e-18 Identities = 124/420 (29%), Positives = 199/420 (47%) Query: 252 ELLGEIRSPSAQKAPIEVQPLPA EGALEEAPAKVEPPTVEETLAEVQPLLPEEAP 306 ELLG+I+ A +A + + A AL E A++E TV+ TL + Sbjct: 236 ELLGQIQGCGAAQAQAQAEARDALKCDVTSALREIRAQLEGHTVQSTLQSEEWFRVRLDR 295 Query: 307 REEARELQLSTAMETPAEEAPTEFQSPLPKETTAEEASAEIQLLAATEPPADETPAEARS 366 EA ++ + AM + EE TE++ L TT E++ L +T+ + +E Sbjct: 296 LSEAAKVN-TDAMRSAQEEI-TEYRRQLQARTT ELEALKSTKESLERQRSELED 347 Query: 367 PLSEE-TSAEEAHAEVQSPLAEETTAEEASA—EIQLLAAIEAPAD-ETPAEAQSPLSEE 422 + S ++A ++ + L T E A+ E Q L ++ D E A + EE Sbjct: 348 RHQVDMASYQDAIQQLDNEL-RNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEE 406 Query: 423 TSAEEAPAEV QSPS-AKGVSIE-EAPLELQPPSGEETT-AEEASAAIQLLA-A 471 P+ + PS + + ++ E +++ Ξ +ET EE + IQ+ Sb j ct: 407 CRIGFGPSPFSLTEGLPKIPSMSTHIKVKΞEEKIKVVEKSEKETVIVEEQTEEIQVTEEV 466 Query: 472 TEASAEEAPAEVQPPPAEEAPAEVQP—PPAEEAPA EVQPPPAEEA—PAEVQPPPA 524 TE +EA E + AEE E PPAEEA + E + P EEA PAE + P Sbjct: 467 TEEEDKEAQGE-EEEEAEEGGEEAATTSPPAEEAASPEKETKSPVKEEAKSPAEAKSPAE 525 Query: 525 EEAPAEVQPPPAEEAPAEVQPPPAEEAPSEVQPPPAEEAPAEVQSLPAE-ETPIE-ETLA 582 ++PAE + P ++PAEV+ P ++P+E + P ++PA V+S PAE ++P E ++ A Sbjct: 526 AKSPAEAKSPAEVKSPAEVKSPAEAKSPAEAKSPAEVKSPATVKS-PAEAKSPAEAKSPA 584 Query: 583 AVHSPPADDVPAEEASVDKHSPPADLLLTEEFPIGEASAEVSPPPSEQTP-EDEALVENV 641 V SP P E S + PA++ E ++ AE P S ++P E ++ E Sbjct: 585 EVKSPATVKSPGEAKSPAEAKSPAEVKSPVE AKSPAEAKSPASVKSPGEAKSPAEAK 641 Query: 642 S-TEFQSPQVAGIP 654 S E +SP P Sbjct: 642 SPAEVKSPA VKSP 655 Score = 253 (38.0 bits), Expect = 9.0e-18, P = 9.0e-18 Identities = 115/364 (31%), Positives = 166/364 (45%) Query: 110 EVTVPVVQEGSAVKKVASAEIEPPSTEKFPAKIQPPLVEEATAKAEPRPAE-ETHVQVQ- 167 E PVV + A K + AE +PP+ K PA+ + P ++ A+A+ PAE ++ V+V+ Sbjct: 705 EAKSPVVAKSPAEAK-SPAEAKPPAEAKSPAEAKSPAEAKSPAEAKS-PAEAKΞPVEVKS 762 Query: 168 PSTEETPDAEAATAVAE—NSVKVQPPPAEEA—PL-VEFPAEIQPPSAEE—SPSVELL 220 P ++P E A ++AE + K + P EE P V+ P + + P EE SP Sbjct: 763 PEKAKSPVKEGAKSLAEAKSPEKAKSPVKEEIKPPAEVKSPEKAKSPMKEEAKSPEKAKT 822 Query: 221 AEILPPSAEESPSEEP—PAEILPPPAEKSPSVELLGEIRSPSAQKAPIE-VQPLPAE— 275 ++ P A+ EE PA+I P KSP+ E E +SP ++ E V P E Sbjct: 823 LDVKSPEAKTPAKEEAKRPADIRSPEQVKSPAKE EAKSPEKEETRTEKVAPKKEEVK 879 Query: 276 GALEEAPAKVEPPTVEETLAEVQPLLPEEAPREEARELQLSTAMETPAEEA-P-TEFQSP 333 +EE AK P VEE E P P+ +E ++ A + AEE P TE Sbjct: 880 SPVEEVKAKEPPKKVEE EKTPATPKTEVKESKKDEAPKEAQKPKAEEKEPLTEKPKD 936 Query: 334 LPKETTAEEASAEIQLLAATEPPADETPAE—ARSPLSEETSAEEAHA-EVQSPLAEETT 390 P E EEA + AA P +ETPA+ + + AE+A A E P +E Sbjct: 937 SPGEAKKEEAKEK KAAA—PEEETPAKLGVKEEAKPKEKAEDAKAKEPSKPSEKEKP 991 Query: 391 A-EEASAEIQLLAAIEAPADETPAEAQSPLSEETSAEEAPAEVQSPSA-KGVSIEEAPLE 448 EE A + E E+ + P + + EE Q PS K E++ Sbjct: 992 KKEEVPAAPEKKDTKEEKTTESKKPEEKPKMQAKAKEEDKGLPQEPSKPKTEKAEKSSST 1051 Query: 449 LQPPSGEETTAEEASAA 465 Q S A E AA Sbjct: 1052 DQKDSQPSEKAPEDKAA 1068 Pedant information for DKFZphtes3_17f10, frame 3 Report for DKFZphtes3_17f10.3 [LENGTH] 710 [MW] 75131.94 [pi] 4.02 [KW] All_Alpha [KW] LOW_COMPLEXITY 34.08 % SEQ MDRSQQTSRTGYWTMMNIPPVEKVDKEQQTYFSESEIVVISRPDSSSTKSKEDALKHKSS SEG PRD cccccccccccccccccccceeehhhhhhhccccceeeeeccccccccchhhhhhhhccc SEQ GKIFASEHPEFQPATNSNEEIGQKNISRTSFTQETKKGPPVLLEDELREEVTVPVVQEGS SEG PRD cceeecccccccccccccccccccccccccceeeecccccchhhhhhhhhheeeeccccc SEQ AVKKVASAEIEPPSTEKFPAKIQPPLVEEATAKAEPRPAEETHVQVQPSTEETPDAEAAT SEG xxxxxxxxxxx PRD chhhhhhhccccccccccccccccchhhhhhhhhccccccceeeecccccccccchhhhh SEQ AVAENSVKVQPPPAEEAPLVEFPAEIQPPSAEESPSVELLAEILPPSAEESPSEEPPAEI SEG xxxx xxxxxxxxxxxxxxxxxxxx PRD hhhhhcccccccccccceeeeccccccccccccccchhhhhhcccccccccccccccccc SEQ LPPPAEKΞPSVELLGEIRSPSAQKAPIEVQPLPAEGALEEAPAKVEPPTVEETLAEVQPL SEG xxxxxx xxxxxxxxxxxxx xxx PRD cccccccccccccccccccccccccccccccccchhhhhcccccccccchhhhhhhhhhc SEQ LPEEAPREEARELQLSTAMETPAEEAPTEFQSPLPKETTAEEASAEIQLLAATEPPADET SEG xxxxxxxxxxxxxxx....xxxxxxxxxx xxxxxxxxxx PRD ccccchhhhhhhhhhhhhhccccccccccccccccchhhhhhhhhhhhhhhhcccccccc SEQ PAEARSPLSEETSAEEAHAEVQSPLAEETTAEEASAEIQLLAAIEAPADETPAEAQSPLS SEG xxxx....xxxxxxxxxxxx xxxxxxxxxxxx xxxx PRD cccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccccccccc SEQ EETSAEEAPAEVQSPSAKGVSIEEAPLELQPPSGEETTAEEASAAIQLLAATEASAEEAP SEG xxxxxxxxxxx xxxxxxxxxxx xxxxxxxx PRD chhhhhcccccccccccceeecccccccccccccchhhhhhhhhhhhhhhhhhhhhhhhc SEQ AEVQPPPAEEAPAEVQPPPAEEAPAEVQPPPAEEAPAEVQPPPAEEAPAEVQPPPAEEAP SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ AEVQPPPAEEAPSEVQPPPAEEAPAEVQSLPAEETPIEETLAAVHSPPADDVPAEEASVD SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD ccccccccccccccccccccccccccccccccccchhhhhhhhhcccccccccccccccc SEQ KHSPPADLLLTEEFPIGEASAEVSPPPΞEQTPEDEALVENVSTEFQSPQVAGIPAVKLGS SEG PRD cccccceeeeeccccccccccccccccccccccchhhhhccccccccccccccccccccc SEQ VVLEGEAKFEEVSKINSVLKDLSNTNDGQAPTLEIESVFHIELKQRPPEL SEG PRD eeeehhhhhhhhccceeeeeeccccccccceeeehhhhhhhhhhcccccc (No Prosite data available for DKFZphtes3_17f10.3) (No Pfam data available for DKFZphtes3_17f10.3) DKFZphtes3_17117 group: metabolism DKFZphtes3_17117 encodes a novel 626 ammo acid protein with similarity to transketaloases (EC 2.2.1.1) . The novel protein contains a ATP/GTP-binding site motif A (P-loop) . It is a new testis- specific transketolase. Transketolase requires thiamin pyrophosphate as cofactor and shows a wide specificity for both reactants, e.g. converts hydroxypyruvate and R-CHO into CO(2) and R- CHOH-CO-CH(2)OH. The new protein can find application in modulation of metabolic pathways involving this transketolase activity and as a new enzyme for biotechnologic production processes. strong similarity to transketolases few EST hits (all from testis or pooled librarys containing testis) testis specific transketolase? Sequenced by GBF Locus : unknown Insert length: 2688 bp Poly A stretch at pos. 2649, polyadenylation signal at pos. 2630 1 GACAAAAGAG AGATGATGGC CAACGACGCC AAGCCCGACG TGAAGACCGT 51 GCAGGTGCTG CGGGACACAG CCAACCGCCT GCGGATCCAT TCCATCAGGG 101 CCACGTGTGC CTCTGGTTCT GGCCAGCTCA CGTCGTGCTG CAGTGCAGCG 151 GAGGTCGTGT CTGTCCTCTT CTTCCACACG ATGAAGTATA AACAGACAGA 201 CCCAGAACAC CCGGACAACG ACCGGTTCAT CCTCTCCAGG GGACATGCTG 251 CTCCTATCCT CTATGCTGCT TGGGTGGAGG TGGGTGACAT CAGTGAATCT 301 GACTTGCTGA ACCTGAGGAA ACTTCACAGC GACTTGGAGA GACACCCTAC 351 CCCGCGATTG CCGTTTGTTG ACGTGGCAAC AGGGTCCCTA GGTCAGGGAT 401 TAGGTACTGC ATGTGGAATG GCTTATACTG GCAAGTACCT TGACAAGGCC 451 AGCTACCGGG TGTTCTGCCT TATGGGAGAT GGCGAATCCT CAGAAGGCTC 501 TGTGTGGGAG GCTTTTGCTT TTGCCTCCCA CTACAACTTG GACAATCTCG 551 TGGCGGTCTT CGACGTGAAC CGCTTGGGAC AAAGTGGCCC TGCACCCCTT 601 GAGCATGGCG CAGACATCTA CCAGAATTGC TGTGAAGCCT TTGGATGGAA 651 TACTTACTTA GTGGATGGCC ATGATGTGGA GGCCTTGTGC CAAGCATTTT 701 GGCAAGCAAG TCAAGTGAAG AACAAGCCTA CTGCTATAGT TGCCAAGACC 751 TTCAAAGGTC GGGGTATTCC AAATATTGAG GATGCAGAAA ATTGGCATGG 801 AAAGCCAGTG CCAAAAGAAA GAGCAGATGC AATTGTCAAA TTAATTGAGA 851 GTCAGATACA GACCAATGAG AATCTCATAC CAAAATCGCC TGTGGAAGAC 901 TCACCTCAAA TAAGCATCAC AGATATAAAA ATGACCTCCC CACCTGCTTA 951 CAAAGTTGGT GACAAGATAG CTACTCAGAA AACATATGGT TTGGCTCTGG 1001 CTAAACTGGG CCGTGCAAAT GAAAGAGTTA TTGTTCTGAG TGGTGACACG 1051 ATGAACTCCA CCTTTTCTGA GATATTCAGG AAAGAACACC CTGAGCGTTT 1101 CATAGAGTGT ATTATTGCTG AACAAAACAT GGTAAGTGTG GCACTAGGCT 1151 GTGCTACACG TGGTCGAACC ATTGCTTTTG CTGGTGCTTT TGCTGCCTTT 1201 TTTACTAGAG CATTCGATCA GCTCCGAATG GGAGCCATTT CTCAAGCCAA 1251 TATCAACCTT ATTGGTTCCC ACTGTGGGGT ATCCACTGGA GAAGATGGAG 1301 TCTCCCAGAT GGCCCTGGAG GATCTAGCCA TGTTCCGAAG CATTCCCAAT 1351 TGTACTGTTT TCTATCCAAG TGATGCCATC TCGACAGAGC ATGCTATTTA 1401 TCTAGCCGCC AATACCAAGG GAATGTGCTT CATTCGAACC AGCCAACCAG 1451 AAACTGCAGT TATTTATACC CCACAAGAAA ATTTTGAGAT TGGCCAGGCC 1501 AAGGTGGTCC GCCACGGTGT CAATGATAAA GTCACAGTAA TTGGAGCTGG 1551 AGTTACTCTC CATGAAGCCT TAGAAGCTGC TGACCATCTT TCTCAACAAG 1601 GTATTTCTGT CCGTGTCATC GACCCATTTA CCATTAAACC CCTGGATGCC 1651 GCCACCATCA TCTCCAGTGC AAAAGCCACA GGCGGCCGAG TTATCACAGT 1701 GGAGGATCAC TACAGGGAAG GTGGCATTGG AGAAGCTGTT TGTGCAGCTG 1751 TCTCCAGGGA GCCTGATATC CTTGTTCATC AACTGGCAGT GTCAGGAGTG 1801 CCTCAACGTG GGAAAACTAG TGAATTGCTG GATATGTTTG GAATCAGTAC 1851 CAGACACATT ATAGCAGCCG TAACACTTAC TTTAATGAAG TAAACTAGGC 1901 TTATTTCTAA AAAGTCAAGT CTATTGGCTT TGGCCCAAAA GCACTGGTAT 1951 CTTTGTATTA AATTCATGTT TATTGTCACA AAACCATTAT TTATACCTAT 2001 ACAGTTGTAC TGTTTCTTTT AAAGCAAAGC CATTTAACAT CTTTCTTCAT 2051 TCCTAATTTG GAAATTAAAG TTTACCTTTC TGTTAATCTA TGTATAAATG 2101 TTACTCTGAG TTATTAATGT GGATTTTAAA ATTGTAAGCA ATAGAATAGG 2151 AAATAAAACA ACTACCTAAT ACAAATATTT CTGATAAGAC TACAAATATC 2201 TGACTGAGCT GGGGATTAAA GTAGAGGTAA CTGTATCTTA AATGAGTATG 2251 ATTTCCTTGT AAGTTAAAAA AATTGAAATT TAATTGTAGA CTTCAATAGT 2301 CCAAGTTTTG AAGGATGTTT GAGCTTTTGT ATAATGCCAT TTATACCTGC 2351 AGTTTTACAG ATAATGTTTG ACTGCAGTTG CCTTGGAAAT TCCTCCAAAG 2401 TTTGCCTTCA TCTCTCCTCT ACAGTTTGGA GGTGATGGTG CAGCAGTGGA 2451 ACATCTCTTG ATGCACCACA CTACTTGTGT TCTGTGAAGT GATGAAAGTA 2501 TAACTGGTTC TAGTTTGCAC ACTACACACA TAGTTTTGTG AAGCTTCAGA 2551 AATGTTTTTT CTTTTCCTTG TGGCCAAACC AGTTTGTTAA TCTGATTATA 2601 TTCATCTGCT AATGATACTA AAGTTAATGT AATAAAGCAT TTAAAAATCA 2651 GAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAA BLAST Results No BLAST result Medlme entries 96214928: Amplification of the transketolase gene in desensitization-resistant mutant YI mouse adrenocortical tumor cells. 99123875: Properties and functions of the thiamm diphosphate dependent enzyme transketolase . Peptide information for frame 1 ORF from 13 bp to 1890 bp; peptide length: 626 Category: strong similarity to known protein Classification: Metabolism Prosite motifs: ATP GTP A (595-603) 1 MMANDAKPDV KTVQVLRDTA NRLRIHSIRA TCASGSGQLT ΞCCSAAEVVS 51 VLFFHTMKYK QTDPEHPDND RFILSRGHAA PILYAAWVEV GDISESDLLN 101 LRKLHSDLER HPTPRLPFVD VATGSLGQGL GTACGMAYTG KYLDKASYRV 151 FCLMGDGESS EGSVWEAFAF ASHYNLDNLV AVFDVNRLGQ SGPAPLEHGA 201 DIYQNCCEAF GWNTYLVDGH DVEALCQAFW QASQVKNKPT AIVAKTFKGR 251 GIPNIEDAEN WHGKPVPKER ADAIVKLIEΞ QIQTNENLIP KSPVEDSPQI 301 SITDIKMTSP PAYKVGDKIA TQKTYGLALA KLGRANERVI VLSGDTMNST 351 FSEIFRKEHP ERFIECIIAE QNMVSVALGC ATRGRTIAFA GAFAAFFTRA 401 FDQLRMGAIS QANINLIGSH CGVSTGEDGV SQMALEDLAM FRSIPNCTVF 451 YPSDAISTEH AIYLAANTKG MCFIRTSQPE TAVIYTPQEN FEIGQAKVVR 501 HGVNDKVTVI GAGVTLHEAL EAADHLSQQG ISVRVIDPFT IKPLDAATII 551 SSAKATGGRV ITVEDHYREG GIGEAVCAAV SREPDILVHQ LAVSGVPQRG 601 KTSELLDMFG ISTRHIIAAV TLTLMK BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_17117, frame 1 SWISSPROT :TKT_MOUΞE TRANSKETOLASE (EC 2.2.1.1) (TK) (P68)., N = 1, Score = 2222, P = 2.5e-230 SWISSPROT :TKT_RAT TRANSKETOLASE (EC 2.2.1.1) (TK) . , N = 1, Score = 2202, P = 3.3e-228 TREMBL:RN09256_1 product: "transketolase"; Rattus norvegicus Sprague-Dawley transketolase mRNA, complete eds., N = 1, Score = 2202, P = 3.3e-228 SWISSPROT :TKT_HUMAN TRANSKETOLASE (EC 2.2.1.1) (TK) . , N = 1, Score = 2200, P = 5.3e-228 >SWISSPROT:TKT_MOUSE TRANSKETOLASE (EC 2.2.1.1) (TK) (P68). Length = 623 HSPs: Score = 2222 (333.4 bits), Expect = 2.5e-230, P = 2.5e-230 Identities = 417/614 (67%), Positives = 501/614 (81%) Query: 7 KPDVKTVQVLRDTANRLRIHSIRATCASGSGQLTSCCSAAEVVSVLFFHTMKYKQTDPEH 66 KPD + +Q L+DTANRLRI SI+AT A+GSG TSCCSAAE+++VLFFHTM+YK DP + Sbjct: 6 KPDQQKLQALKDTANRLRISSIQATTAAGSGHPTSCCSAAEIMAVLFFHTMRYKALDPRN 65 Query: 67 PDNDRFILSRGHAAPILYAAWVEVGDISESDLLNLRKLHSDLERHPTPRLPFVDVATGSL 126 P NDRF+LS+GHAAPILYA W E G + E++LLNLRK+ SDL+ HP P+ F DVATGSL Sbjct: 66 PHNDRFVLSKGHAAPILYAVWAEAGFLPEAELLNLRKISSDLDGHPVPKQAFTDVATGSL 125 Query: 127 GQGLGTACGMAYTGKYLDKASYRVFCLMGDGESSEGSVWEAFAFASHYNLDNLVAVFDVN 186 GQGLG ACGMAYTGKY DKASYRV+C++GDGE SEGSVWEA AFA Y LDNLVA+FD+N Sbjct: 126 GQGLGAACGMAYTGKYFDKASYRVYCMLGDGEVSEGSVWEAMAFAGIYKLDNLVAIFDIN 185 Query: 187 RLGQSGPAPLEHGADIYQNCCEAFGWNTYLVDGHDVEALCQAFWQASQVKNKPTAIVAKT 246 RLGQS PAPL+H DIYQ CEAFGW+T +VDGH VE LC+AF QA K++PTAI+AKT Sbjct: 186 RLGQSDPAPLQHQVDIYQKRCEAFGWHTIIVDGHSVEELCKAFGQA KHQPTAIIAKT 242 Query: 247 FKGRGIPNIEDAENWHGKPVPKERADAIVKLIESQIQTNENLIPKSPVEDSPQISITDIK 306 FKGRGI IED E WHGKP+PK A+ I++ I SQ+Q+ + ++ P ED+P + I +1+ Sbjct: 243 FKGRGITGIEDKEAWHGKPLPKNMAEQIIQEIYSQVQSKKKILATPPQEDAPSVDIANIR 302 Query: 307 MTSPPAYKVGDKIATQKTYGLALAKLGRANERVIVLSGDTMNSTFSEIFRKEHPERFIEC 366 M +PP+YKVGDKIAT+K YGLALAKLG A++R+I L GDT NSTFSE+F+KEHP+RFIEC Sbjct: 303 MPTPPSYKVGDKIATRKAYGLALAKLGHASDRIIALDGDTKNSTFSELFKKEHPDRFIEC 362 Query: 367 IIAEQNMVSVALGCATRGRTIAFAGAFAAFFTRAFDQLRMGAISQANINLIGSHCGVSTG 426 IAEQNMVS+A+GCATR RT+ F FAAFFTRAFDQ+RM AIS++NINL GSHCGVS G Sbjct: 363 YIAEQNMVSIAVGCATRDRTVPFCSTFAAFFTRAFDQIRMAAISESNINLCGSHCGVSIG 422 Query: 427 EDGVSQMALEDLAMFRSIPNCTVFYPSDAISTEHAIYLAANTKGMCFIRTSQPETAVIYT 486 EDG SQMALEDLAMFRS+P TVFYPSD ++TE A+ LAANTKG+CFIRTS+PE A+IY+ Sbjct: 423 EDGPSQMALEDLAMFRSVPMSTVFYPSDGVATEKAVELAANTKGICFIRTSRPENAIIYS 482 Query: 487 PQENFEIGQAKVVRHGVNDKVTVIGAGVTLHEALEAADHLSQQGISVRVIDPFTIKPLDA 546 E+F++GQAKVV +D+VTVIGAGVTLHEAL AA+ L + IS+RV+DPFTIKPLD Sbjct: 483 NNEDFQVGQAKVVLKSKDDQVTVIGAGVTLHEALAAAESLKKDKISIRVLDPFTIKPLDR 542 Query: 547 ATIISSAKATGGRVITVEDHYREGGIGEAVCAAVSREPDILVHQLAVSGVPQRGKTSELL 606 1+ SA+AT GR++TVEDHY EGGIGEAV AAV EP + V +LAVS VP+ GK +ELL Sbjct: 543 KLILDSARATKGRILTVEDHYYEGGIGEAVSAAVVGEPGVTVTRLAVSQVPRSGKPAELL 602 Query: 607 DMFGISTRHIIAAV 620 MFGI 1+ AV Sbjct: 603 KMFGIDKDAIVQAV 616 Pedant information for DKFZphtes3_17117, frame 1 Report for DKFZphtes3_17117.1 [LENGTH] 626 [MW] 67877.52 [pi] 5.90 [HOMOL] SWISSPROT :TKT_MOUSE TRANSKETOLASE (EC 2.2.1.1) (TK) (P68) . 0.0 [FUNCAT] m outer membrane and cell wall [M jannaschn, MJ0681] 3e-48 [FUNCAT] g carbohydrate metabolism and transport [H. influenzae, HI1023] 9e-36 [FUNCAT] 01.05.01 carbohydrate utilization [S. cerevisiae, YPR074c] 5e- -32 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YPR074C] 5e- -32 [FUNCAT] 02.07 pentose-phosphate pathway [S. cerevisiae, YPR074c] 5e- -32 [FUNCAT] 01.01.01 ammo-acid biosynthesis [S. cerevisiae, YPR074C] 5e- -32 [FUNCAT] l lipid metabolism [H. influenzae HI1439] 3e-17 [FUNCAT] c energy conversion [H. influenzae, HI1233] 2e-09 [FUNCAT] 02.01 glycolysis [S. cerevisiae, YBR221C PDB1 - pyruvate dehydrogenase] 2e-05 [FUNCAT] 30.16 mitochondrial organization [S. cerevisiae, YBR221c PDB1 - pyruvate dehydrogenase] 2e-05 [BLOCKS] BL00801F [BLOCKS] BL00801E [BLOCKS] BL00801D Transketolase proteins [BLOCKS] BL00801C Transketolase proteins [BLOCKS] BL00801B Transketolase proteins [BLOCKS] BL00801A Transketolase proteins [SCOP] dltrka2 3.28.1.2.1 Transketolase Transketolase, C-terminal domai le-21 [EC] 1.2.4.1 Pyruvate dehydrogenase (lipoamide) 8e-ll [EC] 1.2.4.4 3-Methyl-2-oxobutanoate dehydrogenase (lipoamide) 4e-10 [EC] 2.2.1.1 Transketolase 0.0 [EC] 2.2.1.3 Formaldehyde transketolase le-20 [PIRKW] transferase 0.0 [PIRKW] flavoprotein 2e-07 [PIRKW] Calvin cycle le-40 [PIRKW] heterotetramer 2e-07 [PIRKW] pentose phosphate pathway 0.0 [PIRKW] magnesium le-40 , [PIRKW] thiamine pyrophosphate 0.0 [PIRKW] oxidoreductase 7e-12 [PIRKW] fatty acid biosynthesis 4e-10 [PIRKW] mitochondrion 2e-07 [PIRKW] peroxisome le-20 [PIRKW] homodimer le-40 [SUPFAM] pyruvate dehydrogenase (lipoamide) alpha chain le-06 [SUPFAM] pyruvate dehydrogenase (lipoamide) beta chain 7e-12 [SUPFAM] ferredoxm 2 [4Fe-4S] -related protein 8e-47 [SUPFAM] thiamine pyrophosphate-binding domain homology 0.0 [SUPFAM] pyruvate dehydrogenase (lipoamide) 6e-08 [SUPFAM] ferredoxm 2[4Fe-4S] homology 8e-47 [SUPFAM] hypothetical protein C2814 2e-21 [SUPFAM] transketolase 0.0 [PROSITE] ATP_GTP_A 1 [PFAM] Transketolase [KW] Alpha_Beta [KW] 3D [KW] LOW COMPLEXITY 3.04 % SEQ MMANDAKPDVKTVQVLRDTANRLRIHSIRATCASGSGQLTSCCSAAEVVΞVLFFHTMKYK SEG IngsB HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH-HHCCCT SEQ QTDPEHPDNDRFILSRGHAAPILYAAWVEVGDISESDLLNLRKLHSDLERHPTPRLPFVD SEG IngsB TTTTTTTTTCEEEETTGGGHHHHHHHHHHHCTTCHHHHHTTTTTTTTTTTTTTTTTTTTC SEQ VATGSLGQGLGTACGMAYTGKYLDKASYRVFCLMGDGESSEGSVWEAFAFASHYNLDNLV SEG IngsB CCCCTTTHHHHHHHHHHHHHHHHCBTTBTTEEEECHHHHHCHHHHHHHHHHHHHCTTTEE SEQ AVFDVNRLGQSGPAPLEHGADIYQNCCEAFGWNTYLVDGHDVEALCQAFWQASQVKNKPT SEG IngsB EEEEECCEETTEEGGGCCCCCHHHHH-HHHCCEEEETTTTTHHHHHHHHHHHHHTTTTCE SEQ AIVAKTFKGRGIPNIEDAENWHGKPVPKERADAIVKLIESQIQTNENLIPKSPVEDSPQI SEG IngsB EEEEECTTTTTTCCHHHHHHHHHHTCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH SEQ SITDIKMTSPPAYKVGDKIATQKTYGLALAKLGRANERVIVLSGDTMNSTFSEIFRKEHP SEG IngsB HHHHHHHHHTCCCTTTTCBCHHHHHHHHHHHHHTTTTTEEEEETTTHHHHCCTTCEECCG SEQ ERFIECIIAEQNMVSVALGCATRGRTIAFAGAFAAFFTRAFDQLRMGAISQANINLIGSH SEG xxxxxxxxxxxxxxxxxxx IngsB GCEEETTTTHHHHHHHHHHHHHHTTTTEEEEEEGGGGGGGHHHHHHHHHHCTTTEEEEEC SEQ CGVSTGEDGVSQMALEDLAMFRSIPNCTVFYPSDAISTEHAIYLAANTKGMCFIRTSQPE SEG IngsB CCGGGTTTTTTTTCCHHHHHHHCTTTTEEECCCCHHHHHHHHHHHTTTTCEEEECCCCCB SEQ TAVIYTPQENFEIGQAK VRHGVNDKVTVIGAGVTLHEALEAADHLSQQGISVRVIDPFT SEG IngsB CCTTTTCHHHHHCC-CEEEETTTTTTEEEEECCHHHHHHHHHHHHHHHHCCCEEEE.... SEQ IKPLDAATIISSAKATGGRVITVEDHYREGGIGEAVCAAVSREPDILVHQLAVSGVPQRG SEG IngsB SEQ KTSELLDMFGISTRHIIAAVTLTLMK SEG IngsB Prosite for DKFZphtes3_17117.1 PS00017 595->603 ATP GTP A PDOC00017 Pfam for DKFZphtes3_17117.1 HMM_NAME Trans etolase HMM *vNtIRιLaMDAVEKANSGHPGaPMGMAPMAHVLWqrMMRHNPNDPrWPN +N++RI ++ A + +SG ++++++A++ VL++++M+++++DP P+ Query 20 ANRLRIHSIRATCASGSGQLTSCCSAAEVVSVLFFHTMKYKQTDPEHPD 68 HMM RDRFVLSNGHaCMLLYsMWHLyGYDMpMWDLkQFRQWHSrTPGHPEIgHT +DRF+LΞ GHA+++LY+ W + G ++++DL+++R++HS++ +HP ++ Query 69 NDRFILSRGHAAPILYAAWVEVGD-ISESDLLNLRKLHSDLERHPTPRLP 117 HMM PGVEVTTGPLGQGIaNaVWMAIAERnLAATYNRPGFDIfDHYTYCFMGDG ++ +V+TG+LGQG++ +++++Y++++ D+++++++C+MGDG Query 118 FV-DVATGSLGQGLG TACGMAYTGKYLDKASYRVFCLMGDG 157 HMM CLMEGISWEACSLAGHMqLGNWIaFYDDNrlSIDGdTdlWFqEDtYakRF + +EG++WEA ++A+H++L+N++A +D NR++++G++++ + D+Y+ + Query 158 ESSEGSVWEAFAFASHYNLDNLVAVFDVNRLGQSGPAPLEHGADIYQNCC 207 HMM EAYGWHVIEVEnDGHDvEelcaAIEeAKaekDRPTLIiCRTVIGYGSPNk EA+GW++ +V DGHDVE++C A+ +A +K++PT+I ++T++G+G+PN Query 208 EAFGWNTYLV—DGHDVEALCQAFWQASQVKNKPTAIVAKTFKGRGIPNI 255 HMM QGTHdWHGAPLGeD* ++ + WHG+P +++ Query 256 EDAENWHGKPVPKE 269 HMM *PqWePnddkIATRKASQqaLeaiGPaLPEfWGGSADLTPSNLTrWKGmv P++++ +DKIAT K+++ AL+++G A +++ +S+D+ +S++++++ ++ Query 311 PAYKV-GDKIATQKTYGLALAKLGRANERVIVLSGDTMNSTFSEIFRKE 358 HMM WFMPPSISTDCynGNWsGRYIHYGIREHgMgAIMNGIAIHGgNFRPYGGT + + R+I++ I+E++M++++ G+A++G+ ++++ G Query 359 H PERFIECIIAEQNMVSVALGCATRGR-TIAFAGA 392 HMM FMMFyDYARPAIRMAALMelPVIWVWTHDSIGLGEDGPTHQPVEHLAHFR F++F+++A++++RM A++ +++++++H++++ GEDG +++++E+LA+FR Query 393 FAAFFTRAFDQLRMGAISQANINLIGSHCGVSTGEDGVSQMALEDLAMFR 442 HMM alPNMsVWRPCDgNETayAWylAvERehTPtiLILSRQNLPQIErNPrqf +IPN +V++P+D+ T+ A YLA+++++ +++++S ++ +++++ P + Query 443 SIPNCTVFYPSDAISTEHAIYLAANTKGM-CFIRTΞQPETAVIYT-PQEN 490 HMM ekvaRGGYVLkDmdnePDVILIATGSEMELAvaAAKlLadEGIkaRVVSM ++++++++V + + + V++I++G+++++A++AA+ L+ +GI +RV+++ Query 491 FEIGQAKVVRHGVN—DKVTVIGAGVTLHEALEAADHLSQQGISVRVIDP 538 HMM PCTeWFD kQDeEYReSVLPDhVPqRVaVEmGvtWCWYKYVGqq ++++++D + ++++R +++DH++ +++++++V ++ +++ + Query 539 FTIKPLDAATIISSAKATGGRVITVEDHYR-EGGIGEAVCAAVSREPDIL 587 HMM GalfGMNrFGESSGKAPpevLYkMFGFTPENI* + +++ +++ ++ +L+ MFG+ +1 Query 588 VHQLAVSGVPQR GKTSELLDMFGISTRHI 616 DKFZphtes3_17nl2 group: transcription factors DKFZphtes3_17nl2.1 encodes a novel 804 amino acid protein which is nearly identical to mouse and trout SOX-LZ . Sox proteins belong to the HMG box superfamily of DNA-bindmg proteins and are involved in the regulation of developmental processes as germ layer formation, organ development and cell type specification. Deletion or mutation of Sox proteins often results in developmental defects and congenital disease in humans. Sox proteins perform their function m a complex interplay with other transcription factors in a manner highly dependent on cell type and promoter context. The new protein is related to the SOX-LZ protein and contains an additional leucin-zipper . The new protein can find application in modulating/blocking the expression of SOX-controlled genes . nearly identical to mouse SOX-LZ complete cDNA, complete eds, few EST hits mouse and trout SOX-LZ, involved in spermatogenesis Sequenced by GBF Locus: unknown Insert length: 2802 bp Poly A stretch at pos. 2692, polyadenylation signal at pos. 2660 1 GGGATAGGAA AGATGAAAGG TCATGGTGAG CTTCAAGGAC ATGAAAGGTT 51 GTTGTCTCAT GTAACAATAG TAGATTGTTT TTTTTCCTAA TATTTCTAGC 101 CAGCCCCTAA GTCAGGTGAT GGAACAAATA CCTACAGTTT AGTCAGGTGA 151 AACAGGAGTG GGTGGAGGAA GGAAAGAAGA AAAATGGGAA GAATGTCTTC 201 CAAGCAAGCC ACCTCTCCAT TTGCCTGTGC AGCTGATGGA GAGGATGCAA 251 TGACCCAGGA TTTAACCTCA AGGGAAAAGG AAGAGGGCAG TGATCAACAT 301 GTGGCCTCCC ATCTGCCTCT GCACCCCATA ATGCACAACA AACCTCACTC 351 TGAGGAGCTA CCAACACTTG TCAGTACCAT TCAACAAGAT GCTGACTGGG 401 ACAGCGTTCT GTCATCTCAG CAAAGAATGG AATCAGAGAA TAATAAGTTA 451 TGTTCCCTAT ATTCCTTCCG AAATACCTCT ACCTCACCAC ATAAGCCTGA 501 CGAAGGGAGT CGGGACCGTG AGATAATGAC CAGTGTTACT TTTGGAACCC 551 CAGAGCGCCG CAAAGGGAGT CTTGCCGATG TGGTGGACAC ACTGAAACAG 601 AAGAAGCTTG AGGAAATGAC TCGGACTGAA CAAGAGGATT CCTCCTGCAT 651 GGAAAAACTA CTTTCAAAAG ATTGGAAGGA AAAAATGGAA AGACTAAATA 701 CCAGTGAACT TCTTGGAGAA ATTAAAGGTA CACCTGAGAG CCTGGCAGAA 751 AAAGAACGGC AGCTCTCCAC CATGATTACC CAGCTGATCA GTTTACGGGA 801 GCAGCTACTG GCAGCGCATG ATGAACAGAA AAAACTGGCA GCGTCACAAA 851 TTGAGAAACA ACGGCAGCAA ATGGACCTTG CTCGCCAACA GCAAGAACAG 901 ATTGCGAGAC AACAGCAGCA ACTTCTGCAA CAGCAGCACA AAATTAATCT 951 CCTGCAGCAA CAGATCCAGG TTCAGGGTCA CATGCCTCCG CTCATGATCC 1001 CAATTTTTCC ACATGACCAG CGGACTCTGG CAGCAGCTGC TGCTGCCCAA 1051 CAGGGATTCC TCTTCCCCCC TGGAATAACA TACAAACCAG GTGATAACTA 1101 CCCCGTACAG TTCATTCCAT CAACAATGGC AGCTGCTGCT GCTTCTGGAC 1151 TCAGCCCTTT ACAGCTCCAG CAGCTCTATG CCGCTCAGCT GGCCAGCATG 1201 CAGGTGTCAC CTGGAGCAAA GATGCCATCA ACTCCACAGC CACCAAACAC 1251 AGCAGGGACG GTCTCACCTA CTGGGATAAA AAATGAAAAG AGAGGGACCA 1301 GCCCTGTAAC TCAAGTTAAG GATGAAGCAG CAGCACAGCC TCTGAATCTC 1351 TCATCCCGAC CCAAGACAGC AGAGCCTGTA AAGTCCCCAA CGTCTCCCAC 1401 CCAGAACCTC TTCCCAGCCA GCAAAACCAG CCCTGTCAAT CTGCCAAACA 1451 AAAGCAGCAT CCCTAGCCCC ATTGGAGGAA GCCTGGGAAG AGGATCCTCT 1501 TTAGGTAAAT GGAAAAGTCA ACACCAGGAA GAGACTTACG AATTAGATAT 1551 CCTATCTAGT CTCAACTCCC CTGCCCTTTT TGGGGATCAG GATACAGTGA 1601 TGAAAGCCAT TCAGGAGGCG CGGAAGATGC GAGAGCAGAT CCAGCGGGAG 1651 CAACAGCAGC AACAGCCACA TGGTGTTGAC GGGAAACTGT CCTCCATAAA 1701 TAATATGGGG CTGAACAGCT GCAGGAATGA AAAGGAAAGA ACGCGCTTTG 1751 AGAATTTGGG GCCCCAGTTA ACGGGAAAGT CAAATGAAGA TGGAAAACTG 1801 GGCCCAGGTG TCATCGACCT TACTCGGCCA GAAGATGCAG AGGGAAGTAA 1851 AGCAATGAAT GGCTCTGCAG CTAAACTACA GCAGTATTAT TGTTGGCCAA 1901 CAGGAGGTGC CACTGTGGCT GAAGCACGAG TCTACAGGGA CGCCCGCGGC 1951 CGTGCCAGCA GCGAGCCACA CATTAAGCGA CCAATGAATG CATTCATGGT 2001 TTGGGCAAAG GATGAGAGGA GAAAAATCCT TCAGGCCTTC CCCGACATGC 2051 ATAACTCCAA CATTAGCAAA ATCTTAGGAT CTCGCTGGAA ATCAATGTCC 2101 AACCAGGAGA AGCAACCTTA TTATGAAGAG CAGGCCCGGC TAAGCAAGAT 2151 CCACTTAGAG AAGTACCCAA ACTATAAATA CAAACCCCGA CCGAAACGCA 2201 CCTGCATTGT TGATGGCAAA AAGCTTCGGA TTGGGGAGTA TAAGCAACTG 2251 ATGAGGTCTC GGAGACAGGA GATGAGGCAG TTCTTTACTG TGGGGCAACA 2301 GCCTCAGATT CCAATCACCA CAGGAACAGG TGTTGTGTAT CCTGGTGCTA 2351 TCACTATGGC AACTACCACA CCATCGCCTC AGATGACATC TGACTGCTCT 2401 AGCACCTCGG CCAGCCCGGA GCCCAGCCTC CCGGTCATCC AGAGCACTTA 2451 TGGTATGAAG ACAGATGGCG GAAGCCTAGC TGGAAATGAA ATGATCAATG 2501 GAGAGGATGA AATGGAAATG TATGATGACT ATGAAGATGA CCCCAAATCA 2551 GACTATAGCA GTGAAAATGA AGCCCCGGAG GCTGTCAGTG CCAACTGAGG 2601 AGTTTTTGTT TGCTGAATTA AAGTACTCTG ACATTTCACC CCCCTCCCCA 2651 ACAAAGAGTT ATTAAAGAGC CCGCATGCAT TTGTGGCTCC ACAATTAAAA 2701 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 2751 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 2801 AA BLAST Results NO BLAST result Medline entries 95311974: A gene that is related to SRY and is expressed in the testes encodes a leucine zipper-containing protein. 96032826: The Sry-related HMG box-containing gene Sox6 is expressed in the adult testis and developing nervous system of the mouse. Peptide information for frame 1 ORF from 184 bp to 2595 bp; peptide length: 804 Category: strong similarity to known protein 1 MGRMSSKQAT SPFACAADGE DAMTQDLTSR EKEEGSDQHV ASHLPLHPIM 51 HNKPHSEELP TLVSTIQQDA DWDΞVLSSQQ RMESENNKLC SLYSFRNTST 101 SPHKPDEGSR DREIMTSVTF GTPERRKGSL ADVVDTLKQK KLEEMTRTEQ 151 EDSSCMEKLL SKDWKEKMER LNTSELLGEI KGTPESLAEK ERQLSTMITQ 201 LISLREQLLA AHDEQKKLAA SQIEKQRQQM DLARQQQEQI ARQQQQLLQQ 251 QHKINLLQQQ IQVQGHMPPL MIPIFPHDQR TLAAAAAAQQ GFLFPPGITY 301 KPGDNYPVQF IPSTMAAAAA SGLSPLQLQQ LYAAQLASMQ VSPGAKMPST 351 PQPPNTAGTV SPTGIKNEKR GTSPVTQVKD EAAAQPLNLS ΞRPKTAEPVK 401 SPTSPTQNLF PAΞKTSPVNL PNKSSIPSPI GGSLGRGSSL GKWKSQHQEE 451 TYELDILSSL NSPALFGDQD TVMKAIQEAR KMREQIQREQ QQQQPHGVDG 501 KLSSINNMGL NSCRNEKERT RFENLGPQLT GKSNEDGKLG PGVIDLTRPE 551 DAEGSKAMNG SAAKLQQYYC WPTGGATVAE ARVYRDARGR ASSEPHIKRP 601 MNAFMVWAKD ERRKILQAFP DMHNSNISKI LGSRWKSMSN QEKQPYYEEQ 651 ARLSKIHLEK YPNYKYKPRP KRTCIVDGKK LRIGEYKQLM RSRRQEMRQF 701 FTVGQQPQIP ITTGTGVVYP GAITMATTTP SPQMTSDCSS TSASPEPSLP 751 VIQSTYGMKT DGGSLAGNEM INGEDEMEMY DDYEDDPKSD YSSENEAPEA 801 VSAN BLASTP hits Entry MMSOXLZ2_l from database TREMBL: product: "SOX-LZ"; Mouse mRNA for SOX-LZ, complete eds. Score = 3910, P = O.Oe+00, identities = 764/801, positives = 774/801 Entry 151083 from database PIR: SOX-LZ - rainbow trout Score = 1774, P = l.le-287, identities = 365/532, positives = 431/532 Entry S59121 from database PIR: SOX6 protein - mouse Score = 2319, P = 1.2e-240, identities = 489/660, positives = 527/660 Entry AB006330_1 from database TREMBL: gene: "mSox5L"; product: "SOX5"; Mus musculus mSox5L mRNA, complete eds . Score = 1212, P = 8.9e-209, identities = 274/457, positives = 324/457 Entry MMU010604_1 from database TREMBL: gene: "sox5"; product: "L-Sox5 protein"; Mus musculus mRNA for transcription factor L-Sox5 Score = 879, P = 4.2e-195, identities = 190/281, positives = 218/281 Alert BLASTP hits for DKFZphtes3_17nl2, frame 1 No Alert BLASTP hits found Pedant information for DKFZphtes3_17nl2, frame 1 Report for DKFZphtes3_17nl2.1 [LENGTH] 804 [MW] 89332.69 [pi] 6.97 [HOMOL] TREMBL :MMSOXLZ2 1 product: "SOX-LZ"; Mouse mRNA for SOX-LZ, complete eds. 0.0 [FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YKL032c] 8e-07 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YKL032c] 8e-07 [FUNCAT] 01.07.07 regulation of vitamins, cofactors, and prosthetic groups [S. cerevisiae, YPR065W] 5e-06 [FUNCAT] 03.04 budding, cell polarity and filament formation [Ξ. cerevisiae, YBR089c-a] 7e-06 [FUNCAT] 30.13 organization of chromosome structure [S. cerevisiae, YBR089c-a] 7e-06 [FUNCAT] 03.01 cell growth [S. cerevisiae, YBR089c-a] 7e-06 [FUNCAT] 03.16 dna synthesis and replication [S. cerevisiae, YMR072w] 2e-04 [FUNCAT] 30.16 mitochondrial organization [S. cerevisiae, YMR072w] 2e-04 [SCOP] dlhmf 1.20.1.1.1 HMG1, fragments A and B [rat/hamster (Rattu le-13 [SCOP] dllefa_ 1.20.1.1.6 Lymphoid enhancer-binding factor, LEFl [mous 4e-15 [SCOP] dlhrya_ 1.20.1.1.4 SRY [Human (Homo sapiens) le-11 [PIRKW] DNA binding 4e-94 [PIRKW] T-cell receptor 4e-07 [PIRKW] leucine zipper le-38 [PIRKW] alternative splicing 2e-07 [PIRKW] transcription factor 4e-16 [PIRKW] transcription regulation le-12 [SUPFAM] HMG box homology 0.0 [SUPFAM] unassigned HMG box proteins 4e-94 [PROSITE] ATP_GTP_A 1 [PROSITE] LEUCINE_ZIPPER 1 [PROSITE] MYRISTYL 6 [PROSITE] AMIDATION 1 [PROSITE] CAMP_PHOSPHO_SITE 2 [PROSITE] CK2_PHOSPHO_SITE 14 [PROSITE] PKC_PHOSPHO_SITE 10 [PROSITE] ASN_GLYCOSYLATION 6 [PFAM] HMG (high mobility group) box [KW] Irregular [KW] 3D [KW] LOW_COMPLEXITY 13.81 % [KW] COILED COIL 3.48 % SEQ MGRMSSKQATSPFACAADGEDAMTQDLTSREKEEGSDQHVASHLPLHPIMHNKPHSEELP SEG COILS lnhm- SEQ TLVSTIQQDADWDSVLSSQQRMESENNKLCSLYSFRNTSTSPHKPDEGSRDREIMTSVTF SEG COILS lnhm- SEQ GTPERRKGSLADVVDTLKQKKLEEMTRTEQEDSSCMEKLLSKDWKEKMERLNTSELLGEI SEG COILS lnhm- SEQ KGTPESLAEKERQLSTMITQLISLREQLLAAHDEQKKLAASQIEKQRQQMDLARQQQEQI SEG xxxxxxxxxxxxxxx COILS CCCCCC lnhm- SEQ ARQQQQLLQQQHKINLLQQQIQVQGHMPPLMIPIFPHDQRTLAAAAAAQQGFLFPPGITY SEG xxxxxxxxxxxxxxxxxxxxxx xxxxxx COILS CCCCCCCCCCCCCCCCCCCCCC lnhm- SEQ KPGDNYPVQFIPSTMAAAAASGLSPLQLQQLYAAQLASMQVSPGAKMPSTPQPPNTAGTV SEG xxxxxxxxxxxx COILS lnhm- SEQ SPTGIKNEKRGTSPVTQVKDEAAAQPLNLSSRPKTAEPVKSPTSPTQNLFPASKTSPVNL SEG COILS lnhm- SEQ PNKSSIPSPIGGSLGRGSSLGKWKSQHQEETYELDILSSLNSPALFGDQDTVMKAIQEAR SEG ... xxxxxxxxxxxxxxxxxx COILS lnhm- SEQ KMREQIQREQQQQQPHGVDGKLSSINNMGLNSCRNEKERTRFENLGPQLTGKSNEDGKLG SEG .. xxxxxxxxxxxx COILS lnhm- SEQ PGVIDLTRPEDAEGSKAMNGSAAKLQQYYCWPTGGATVAEARVYRDARGRASΞEPHIKRP SEG COILS lnhm- CCC SEQ MNAFMVWAKDERRKILQAFPDMHNSNISKILGSRWKSMSNQEKQPYYEEQARLSKIHLEK SEG X COILS lnhm- CCCHHHHHHHHHHHHHHHTTTTCCHHHHHHHHHHHTTTTTTHHHHHHHHHHHHHHHHHHH SEQ YPNYKYKPRPKRTCIVDGKKLRIGEYKQLMRSRRQEMRQFFTVGQQPQIPITTGTGVVYP SEG xxxxxxxxxxxx COILS lnhm- HHHTTTTTTT SEQ GAITMATTTPSPQMTSDCSSTSASPEPSLPVIQΞTYGMKTDGGSLAGNEMINGEDEMEMY SEG xxxxxxx COILS lnhm- SEQ DDYEDDPKSDYΞSENEAPEAVSAN SEG xxxxxx COILS lnhm- Prosite for DKFZphtes3_17nl2.1 PS00001 97->101 ASN_GLYCOSYLATION PDOC00001 PS00001 172-M76 ASN_GLYCOSYLATION PDOC00001 PS00001 388->392 ASN_GLYCOSYLATION PDOC00001 PS00001 422->426 ASN_GLYCOSYLATION PDOC00001 PS00001 559->563 ASN_GLYCOSYLATION PDOC00001 PΞ00001 626->630 ASN_GLYCOSYLATION PDOC00001 PS00004 126->130 CAMP_PHOSPHO_SITE PDOC00004 PS00004 369->373 CAMP_PHOSPHO_ΞITE PDOC00004 PS00005 5->8 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 28->31 PKC_PHOSPHO_SITE PDOC00005 PS00005 94->97 PKC_PHOSPHO_SITE PDOC00005 PS00005 136->139 PKC_PHOSPHO_SITE PDOC00005 PS00005 203->206 PKC_PHOSPHO_SITE PDOC00005 PS00005 299->302 PKC_PHOSPHO_SITE PDOC00005 PS00005 390->393 PKC_PHOSPHO_SITE PDOC00005 PS00005 512->515 PKC_PHOSPHO_SITE PDOC00005 PS00005 530->533 PKC_PHOSPHO_SITE PDOC00005 PS00005 692->695 PKC_PHOSPHO_SITE PDOC00005 PS00006 28->32 CK2_PHOSPHO_SITE PDOC00006 PS00006 129->133 CK2_PHOSPHO_SITE PDOC00006 PS00006 146->150 CK2_PHOSPHO_SITE PDOC00006 PS00006 148->152 CK2_PHOSPHO_SITE PDOC00006 PS00006 154->158 CK2_PHOSPHO_SITE PDOC00006 PS00006 186->190 CK2_PHOSPHO_SITE PDOC00006 PS00006 203->207 CK2_PHOSPHO_SITE PDOC00006 PS00006 221->225 CK2_PHOSPHO_SITE PDOC00006 PS00006 520->524 CK2_PHOSPHO_SITE PDOC00006 PS00006 533->537 CK2_PHOSPHO_SITE PDOC00006 PS00006 547->551 CK2_PHOSPHO_SITE PDOC00006 PS00006 577->581 CK2_PHOSPHO_SITE PDOC00006 PS00006 639->643 CK2_PHOSPHO_SITE PDOC00006 PS00006 793->797 CK2_PHOSPHO_SITE PDOC00006 PS00008 182->188 MYRISTYL PDOC00008 PS00008 431->437 MYRISTYL PDOC00008 PS00008 437->443 MYRISTYL PDOC00008 PS00008 509->515 MYRISTYL PDOC00008 PS00008 575->581 MYRISTYL PDOC00008 PS00008 762->768 MYRISTYL PDOC00008 PS00009 677->681 AMIDATION PDOC00009 PS00017 526->534 ATP_GTP_A PDOC00017 PS00029 187->209 LEUCINE ZIPPER PDOC00029 Pfam for DKFZphtes3_17nl2.1 HMM_NAME HMG (high mobility group) box HMM *PKRPMNAYMLWMQEMRekIKaENPNdMhNtEISKMιGEMWKnMsEEEKm +KRPMNA+M+W+++ R+KI + P DMHN++ISK++G +WK+MS +EK+ Query 597 IKRPMNAFMVWAKDERRKILQAFP-DMHNSNISKILGSRWKSMSNQEKQ 644 HMM PYEdMAeeEKqRYMKEMPeYK* PY+++ +++ + +++ +P+YK Query 645 PYYEEQARLSKIHLEKYPNYK 665 DKFZphtes3 17nl8 group: intracellular transport and trafficking DKFZphtes3_17nl8 encodes a novel 782 amino acid protein with weak partial similarity to known proteins . The novel protein contains a ATP/GTP-binding site motif A (P-loop) and a TonB-dependent receptor protein signature 1. In E. coli, the tonB protein interacts with outer membrane receptor proteins that mediate uptake of specific substrates into the periplasmic space. In the absence of tonB these receptors bind their substrates but do not carry out active transport. The novel protein seems to be involved m ATP-dependent transport of substances into the cell. The new protein can find application in modulation of cell-permeability and transport of suitable substrates into the cell. unknown receptor protein containes TONB_DEPENDENT_REC_l Pattern and ATP_GTP_A Pattern, Sequenced by GBF Locus: unknown Insert length: 2853 bp Poly A stretch at pos. 2806, no polyadenylation signal found 1 GTCCTTTTAA GTCAGTAAAT TGAACTAAGT CGGTTATTCG GCAAGCAGTT 51 CCTATAAAAA ACTACATGGC TAAGGTTCTT AATGATTGAC CACAAGCAGA 101 TCTTTCACCC TCGGATCTCT AGCTACAAAA GGTCCCCACA CTGAAGAAGC 151 CACTACCTCC ACCACCACCA GCACCACCAC GTCCAGTGCT GCTGGCAACC 201 ACTGGGGCAG CCAAGCGCTC CACCCTCTCT CCCACCATGG CCCGTCAGGT 251 GCGCACCCAC CAGGAGACCC TGAACAGGTT TCAGCAGCAG TCCATCCACC 301 TGCTGACGGA GCTCCTCAGA CTGAAGATGA AGGCCATGGT GGAGTCTATG 351 TCGGTGGGTG CCAACCCCTT GGACATCACC AGGCGCTTTG TGGAGGCCAG 401 CCAGCTCCTC CACCTCAATG CCAAGGAGAT GGCCTTCAAC TGCCTGATCA 451 GCACAGCCGG GAGAAGTGGC TACAGCAGCG GACAGTTGTG GAAAGAGTCC 501 CTCGCAAACA TGTCCGCCAT TGGGGTGAAC TCGCCTTACC AGCTGATCTA 551 CCACTCTTCC ACAGCCTGTC TGAGCTTTTC TCTCTCTGCT GGAAAAGAAG 601 CCAAGAAGAA AATAGGCAAA TCTAGAACTA CAGAAGATGT CAGCATGCCG 651 CCCCTGCATC GAGGAGTGGG AACCCCTGCC AACAGCCTGG AGTTCAGCGA 701 CCCCTGCCCT GAGGCCCGGG AGAAGCTGCA GGAGTTGTGT CGCCACATAG 751 AAGCTGAAAG GGCCACATGG AAAGGGAGGA ATATCTCCTA CCCCATGATC 801 TTACGAAACT ACAAGGCAAA GATGCCCTCT CATCTAATGT TGGCCCGCAA 851 AGGAGACTCT CAGACCCCGG GTTTACATTA CCCTCCCACT GCAGGTGCTC 901 AGACTCTCAG CCCCACCTCT CACCCATCTT CTGCCAACCA TCATTTCAGT 951 CAGCATTGTC AAGAGGGGAA GGCACCCAAG AAGGCCTTCA AGTTTCATTA 1001 CACCTTCTAT GATGGCTCCT CCTTCGTTTA CTATCCCTCT GGAAACGTCG 1051 CTGTATGTCA GATCCCCACA TGCTGCAGAG GGAGAACCAT CACCTGCCTC 1101 TTTAATGACA TACCTGGATT CTCCTTGCTG GCCCTATTCA ATACTGAAGG 1151 CCAGGGCTGT GTTCACTACA ACCTAAAAAC CAGTTGCCCA TATGTCTTAA 1201 TCTTGGATGA GGAAGGTGGG ACCACCAATG ACCAGCAGGG CTATGTAGTC 1251 CACAAGTGGA GCTGGACTTC CAGGACAGAG ACCCTGCTTT CCCTGGAATA 1301 CAAGGTGAAT GAGGAAATGA AACTAAAGGT ACTGGGACAG GACTCCATCA 1351 CAGTCACCTT CACCTCCCTG AATGAGACAG TAACACTCAC TGTGTCGGCC 1401 AACAATTGTC CCCATGGAAT GGCATATGAC AAACGGCTGA ACCGCAGAAT 1451 CAGCAACATG GACGACAAGG TGTATAAGAT GAGCCGAGCC CTGGCTGAGA 1501 TCAAGAAGCG GTTTCAGAAG ACAGTGACTC AGTTCATTAA TTCTATCTTG 1551 CTGGCCGCAG GTCTGTTTAC CATTGAATAT CCCACCAAAA AGGAGGAGGA 1601 AGAATTTGTT CGGTTCAAGA TGAGATCCAG AACTCATCCC GAGCGGCTCC 1651 CCAAGCTAAG TTTATACTCA GGAGAAAGTC TTTTACGATC TCAGTCAGGC 1701 CACCTGGAAT CCTCAATTGC AGAGACTTTG AAGGATGAGC CTGAGTCTGC 1751 TCCTGTGAGC CCAGTTCGGA AGACCACCAA AATCCACACC AAAGCCAAGG 1801 TCACATCCAG AGGGAAGGCC CGCGAGGGGC GCAGCCCCAC CAGGTGGGCG 1851 GCCTTGCCCT CAGACTGCCC GCTGGTGCTG CGGAAGCTCA TGCTCAAGGA 1901 AGACACCCGT GCTGGCTGCA AGTGCCTGGT GAAGGCGCCC CTGGTCTCTG 1951 ACGTGGAGCT GGAGCGCTTC CTGTTGGCGC CCCGAGACCC CAGCCAAGTG 2001 CTGGTGTTTG GGATCATCTC AAGCCAGAAC TACACCAGCA CTGGGCAGCT 2051 CCAGTGGCTG CTGAACACTC TCTACAACCA CCAGCAGCGG GGCCGTGGCT 2101 CCCCCTGCAT CCAGTGCCGG TATGACTCCT ACCGCCTGCT GCAGTATGAC 2151 CTGGACAGCC CCCTGCAGGA GGACCCTCCC CTGATGGTGA AGAAGAACTC 2201 TGTGGTGCAG GGGATGATTC TGATGTTTGC CGGGGGGAAG CTCATTTTTG 2251 GGGGCCGTGT TTTGAATGGA TATGGCCTCA GCAAGCAGAA TCTGCTGAAA 2301 CAGATCTTCC GGTCTCAACA GGATTACAAG ATGGGCTACT TCCTGCCGGA 2351 TGACTACAAA TTCAGTGTTC CCAACTCTGT CCTGAGCCTG GAGGATTCTG 2401 AATCAGTCAA GAAAGCCGAG TCAGAAGATA TCCAAGGAAG CAGCTCCTCA 2451 TTGGCCCTGG AAGACTATGT GGAtJAAGGAG TTATCTCTGG AGGCTGAGAA 2501 GACAAGAGAG CCTGAAGTGG AGCTACATCC TCTCAGCAGG GACAGCAAGA 2551 TAACTAGTTG GAAGAAGCAG GCCTfJtAAGA AGTAGCGCCA TCCTGGCAGC 2601 AGCCAAGTGA GCCAGGCCCC GGCCCGGGGT GCTGGGGCTT CTTGCCAGCC 2651 CAGCCCTGCC TCCCCGGTCT CCCACCCTGΓ CCTCCAAGCT TCTATAATAA 2701 ACCAGCGGGC CTCCAGCATT GGGGTGAGGC TCTGGGGAAG GACAAAAAAA 2751 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAGGG 2801 CGGCCGAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAGGGCGG 2851 CCG BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 3 ORF from 237 bp to 2582 bp; peptide length: 782 Category: putative protein Prosite motifs: ATP_GTP_A (122-130) TONB DEPENDENT REC 1 (1-44) 1 MARQVRTHQE TLNRFQQQSI HLLTELLRLK MKAMVESMΞV GANPLDITRR 51 FVEASQLLHL NAKEMAFNCL ISTAGRSGYS SGQLWKESLA NMSAIGVNSP 101 YQLIYHSSTA CLSFSLSAGK EAKKKIGKSR TTEDVSMPPL HRGVGTPANS 151 LEFSDPCPEA REKLQELCRH IEAERATWKG RNISYPMILR NYKAKMPSHL 201 MLARKGDSQT PGLHYPPTAG AQTLSPTSHP SSANHHFSQH CQEGKAPKKA 251 FKFHYTFYDG SSFVYYPSGN VAVCQIPTCC RGRTITCLFN DIPGFSLLAL 301 FNTEGQGCVH YNLKTSCPYV LILDEEGGTT NDQQGYVVHK WSWTSRTETL 351 LSLEYKVNEE MKLKVLGQDS ITVTFTSLNE TVTLTVSANN CPHGMAYDKR 401 LNRRISNMDD KVYKMSRALA EIKKRFQKTV TQFINSILLA AGLFTIEYPT 451 KKEEEEFVRF KMRSRTHPER LPKLΞLYSGE SLLRΞQSGHL ESSIAETLKD 501 EPESAPVSPV RKTTKIHTKA KVTSRGKARE GRSPTRWAAL PSDCPLVLRK 551 LMLKEDTRAG CKCLVKAPLV SDVELERFLL APRDPSQVLV FGIISSQNYT 601 STGQLQWLLN TLYNHQQRGR GSPCIQCRYD SYRLLQYDLD SPLQEDPPLM 651 VKKNSVVQGM ILMFAGGKLI FGGRVLNGYG LSKQNLLKQI FRSQQDYKMG 701 YFLPDDYKFS VPNSVLSLED SESVKKAESE DIQGSSSSLA LEDYVEKELS 751 LEAEKTREPE VELHPLSRDS KITSWKKQAS KK BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_17nl8, frame 3 No Alert BLASTP hits found Pedant information for DKFZphtes3_17nl8, frame 3 Report for DKFZphtes3_17nl8.3 [LENGTH] 782 [MW] 88030.16 [pi] 9.22 [BLOCKS] BL00286 Squash family of serme protease inhibitors proteins [PROSITE] ATP_GTP_A 1 [PROSITE] MYRISTYL 4 [PROSITE] CAMP_PHOSPHO_SITE 3 [PROSITE] CK2_PHOSPHO_SITE 14 [PROSITE] PROKAR_LIPOPROTEIN 1 [PROSITE] TONB_DEPENDENT_REC_l 1 [PROSITE] PKC_PHOSPHO_ΞITE 10 [PROSITE] ASN_GLYCOSYLATION 4 [KW] Alpha_Beta SEQ MARQVRTHQETLNRFQQQSIHLLTELLRLKMKAMVESMSVGANPLDITRRFVEASQLLHL PRD ccchhhhhhhhhhhchhhhhhhhhhhhhhhhhhhhhhhhcccccchhhhhhhhhhhhhhh SEQ NAKEMAFNCLISTAGRSGYSSGQLWKESLANMSAIGVNSPYQLIYHSSTACLSFSLSAGK PRD hhhhhhhhhhhhcccccccccccchhhhhhhhhcccccccceeeecccceeeecccccch SEQ EAKKKIGKSRTTEDVSMPPLHRGVGTPANSLEFSDPCPEAREKLQELCRHIEAERATWKG PRD hhhhhhhcccccccccccccccccccccccccccccccchhhhhhhhhhhhhhhhhhhhc SEQ RNISYPMILRNYKAKMPSHLMLARKGDΞQTPGLHYPPTAGAQTLSPTSHPSSANHHFSQH PRD cccccchhhhhhhhcccccceeeccccccccccccccccccccccccccccccccccccc SEQ CQEGKAPKKAFKFHYTFYDGSSFVYYPSGNVAVCQIPTCCRGRTITCLFNDIPGFSLLAL PRD ccccccchhhhheeeecccccceeeecccceeeeeccccccceeeeeeccccccceeeee SEQ FNTEGQGCVHYNLKTSCPYVLILDEEGGTTNDQQGYVVHKWSWTSRTETLLSLEYKVNEE PRD ecccccceeeeeccccccceeeeecccccccccceeeeeeecccchhhhhhhhhhhhhhh SEQ MKLKVLGQDSITVTFTSLNETVTLTVSANNCPHGMAYDKRLNRRISNMDDKVYKMSRALA PRD hhhhhhccceeeeeeccccceeeeeeecccccccchhhhhhhhhhhcccchhhhhhhhhh SEQ EIKKRFQKTVTQFINSILLAAGLFTIEYPTKKEEEEFVRFKMRSRTHPERLPKLSLYSGE PRD hhhhhhhhhhhhhhhhhhhhcccceeecccchhhhhhhhhhhccccccccccceeeeccc SEQ SLLRSQSGHLESSIAETLKDEPESAPVSPVRKTTKIHTKAKVTSRGKAREGRSPTRWAAL PRD eeeecccccchhhhhhhhhccccccccccccccccccceeeeeccccccccccccccccc SEQ PSDCPLVLRKLMLKEDTRAGCKCLVKAPLVSDVELERFLLAPRDPSQVLVFGIISSQNYT PRD ccccchhhhhhhhhhhhhhhhhhhhccccchhhhhhhhhccccccceeeeeeeecccccc SEQ STGQLQWLLNTLYNHQQRGRGSPCIQCRYDSYRLLQYDLDSPLQEDPPLMVKKNSVVQGM PRD ccchhhhhhhhhhhhhcccccccceeeecccccceeecccccccccccccccccchhhhh SEQ ILMFAGGKLIFGGRVLNGYGLSKQNLLKQIFRSQQDYKMGYFLPDDYKFSVPNSVLSLED PRD heeeccccccccccccccccccchhhhhhhhhhhhhccccccccccceeecccceeeccc SEQ SESVKKAESEDIQGSSSSLALEDYVEKELSLEAEKTREPEVELHPLSRDSKITSWKKQAS PRD chhhhhhhhcccccccccchhhhhhhhhhhhhhhhhcccceeeccccccccccccccccc SEQ KK PRD cc Prosite for DKFZphtes3_17nl8.3 PS00001 91->95 ASN_GLYCOSYLATION PDOC00001 PS00001 182->186 ASN_GLYCOSYLATION PDOC00001 PS00001 379->383 ASN_GLYCOSYLATION PDOC00001 PS00001 598->602 ASN_GLYCOSYLATION PDOC00001 PS00004 403->407 CAMP_PHOSPHO_SITE PDOC00004 PS00004 511->515 CAMP_PHOSPHO_SITE PDOC00004 PS00004 652->656 CAMP_PHOSPHO_SITE PDOC00004 PS00005 48->51 PKC_PHOSPHO_SITE PDOC00005 PS00005 177->180 PKC_PHOSPHO_SITE PDOC00005 PS00005 344->347 PKC_PHOSPHO_SITE PDOC00005 PS00005 450->453 PKC_PHOSPHO_SITE PDOC00005 PS00005 497->500 PKC_PHOSPHO_SITE PDOC00005 PS00005 513->516 PKC_PHOSPHO_SITE PDOC00005 PS00005 523->526 PKC_PHOSPHO_SITE PDOC00005 PS00005 631->634 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 723->726 PKC_PHOSPHO_SITE PDOC00005 PS00005 774->777 PKC_PHOSPHO_SITE PDOC00005 PS00006 7->ll CK2_PHOSPHO_SITE PDOC00006 PS00006 131->135 CK2_PHOSPHO_SITE PDOC00006 PS00006 256->260 CK2_PHOSPHO_SITE PDOC00006 PS00006 329->333 CK2_PHOSPHO_ΞITE PDOC00006 PS00006 345->349 CK2_PHOSPHO_SITE PDOC00006 PS00006 377->381 CK2_PHOSPHO_SITE PDOC00006 PS00006 406->410 CK2_PHOSPHO_SITE PDOC00006 PS00006 450->454 CK2_PHOSPHO_SITE PDOC00006 PS00006 466->470 CK2_PHOSPHO_SITE PDOC00006 PS00006 493->497 CK2_PHOSPHO_SITE PDOC00006 PS00006 497->501 CK2_PHOSPHO_SITE PDOC00006 PS00006 571->575 CK2_PHOSPHO_SITE PDOC00006 PS00006 693->697 CK2_PHOΞPHO_SITE PDOC00006 PS00006 717->721 CK2_PHOSPHO_SITE PDOC00006 PS00008 145->151 MYRISTYL PDOC00008 PS00008 327->333 MYRISTYL PDOC00008 PS00008 592->598 MYRISTYL PDOC00008 PS00008 734->740 MYRISTYL PDOC00008 PS00013 101->112 PROKAR_LIPOPROTEIN PDOC00013 PS00017 122->130 ATP_GTP_A PDOC00017 PS00430 l->44 TONB DEPENDENT REC 1 PDOC00354 (No Pfam data available for DKFZphtes3_17nl8.3) DKFZphtes3_18f3 group: testes derived DKFZphtes3_18f3 encodes a novel 248 amino acid protein with partial similarity to human TNF- mducible protein CG12-1. The novel protein contains two leucine zippers. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . similarity to TNF-mducible protein CG12-1 Sequenced by MediGenomix Locus : unknown Insert length: 4608 bp Poly A stretch at pos. 4570, polyadenylation signal at pos. 4550 1 GACAGAAGTG AATGGGAATG GAGAGGCCGG CGGCCCGGGA GCCGCATGGG 51 CCCGACGCGC TGCGGCGCTT CCAGGGACTG CTGCTGGACC GCCGAGGCCG 101 GCTGCACCGC CAGGTGCTGC GCCTGCGCGA GGTGGCCCGG CGCCTGGAGC 151 GCCTGCGCAG GCGCTCCCTC GTAGCCAACG TGGCCGGCAG CTCGCTGAGC 201 GCAACGGGCG CCCTCGCCGC CATCGTGGGG CTCTCGCTCA GCCCGGTCAC 251 CCTGGGGACC TCGCTGCTGG TGTCGGCCGT GGGGCTGGGG GTGGCCACAG 301 CCGGAGGGGC CGTCACCATC ACGTCCGATC TCTCGCTGAT CTTCTGCAAC 351 TCCCGGGAGC TGCGGAGGGT GCAGGAGATC GCGGCCACCT GCCAGGACCA 401 GATGCGAGAG ATCCTGAGCT GCCTCGAGTT TTTCTGCCGC TGGCAGGGCT 451 GCGGGGACCG CCAGCTGCTG CAGTGCGGGA GGAACGCCTC CATCGCCCTG 501 TACAATTCTG TCTACTTCAT CGTCTTCTTT GGCTCACGTG GCTTCCTCAT 551 CCCCAGGCGG GCGGAGGGGG ACACCAAGGT TAGCCAGGCC GTGCTGAAGG 601 CCAAGATTCA GAAACTGGCC GAGAGCCTGG AGTCCTGCAC CGGGGCTCTG 651 GACGAACTCA GCGAGCAGCT GGAGTCTCGG GTTCAGCTCT GCACCAAGTC 701 CAGTCGTGGC CACGACCTCA AGATCTCTGC TGACCAGCGT GCAGGGCTGT 751 TTTTCTGAGA ACATCCTTTC CCCCTAATGA CCGAGGCCAG CAAATCATCC 801 TCATGGGATG CTCCAGAATT TGTAGCTCCC TTAGGAAAAC ACCAAGCTGG 851 GTTAGGAGCC GAAGGCAAAG GATGAGAAAA ACTGTTTTTG AAGTGGGCAG 901 GTCCCCAAAG CCCTTCTTTT CCCATCACTG TGACATCTGC CTGGGCTTGA 951 GTGCTACGGA CTTTTCAGTC TTCCTAGTGG AAAAATGTGA CCCAAAAACT 1001 CCTTTTCCTT TATCAAAAAC TTTCTGTCTA AACACAGCTG GGCAGGCACT 1051 CCTGTTTTAA AGTTATTTCG GGGTCCCTGA CCCTGCCCTG GTGGCTTGGC 1101 CTGAGACTGG AGAGAGTGCC ATCCTCTGGG TCCTCTCCAA GTCCTACTAG 1151 TCTTTGAAGT CCTCAAAATG TGCGTGAGGA AGGCATTTGC CTCTATTCCA 1201 GAATTTCTGA TACAAAGAAC TCCAGAATCC AGAGCAAATC AGCCCTTCTC 1251 TGAACGTTGT AGGATGGTTC AGAACCCAGA GAGGACCCTG GTGCTGATAT 1301 CTCCTCCTCT TCCCTTTCCC CTCAGCTTAC TTACTCCCAG ATGCGGCCTG 1351 GGTATGAAGT AGGCCTTTCC TGAGTGGCTC CCAATCCAGT CCTCCAAGTA 1401 CTCAGAGGGG AAGCCCGTGA AGCCGTCATC TAAGTCCTGC TCCCTCACAT 1451 GAAGCTGAGG GCCAGATAGA TGGAGCGACT GCCAACTTCA TTTCCCGACA 1501 TCATTGTGTT CAGAAGAGAG TGATGGGTTT TGAGTTAGAC AGTCCTGGGC 1551 TTGAGACAGG CTTTGTCACT ACTGTGTGAG TGTAGCCACC TAATCTCTCT 1601 GAGACTGTGT AAAACAAAGA TGATAAAATC TCACCCTGTT GTGAGATATT 1651 AAATGAGCCA AAGTGCCTAG CATGATGGTG CTGGCTCATA TAGTGTAGTC 1701 CCTGGAATGG CAAATTAACA TCACCCAGGA ACTTGTTAGA AAGGCAAATT 1751 CTTGGACACA ACCCTCCTGA TTTATGGAAT CAGAAACTCT GGCTGTGGGG 1801 CCCAGCAACC TGAGTTTAAA CAATTTCTCT GGGTGGTTCT GCGGCACACT 1851 AAGGTTTGAA AATCACTACA ACAAATGCTA ACTTCTAATC CCCTTGATGA 1901 GCTTTCACGA AGTCTCACGG CTTCTCTAGG GACTCCATGG TCTTCAGAGT 1951 CGTTCACAGA TGACCAAGGA CAGACTGTGT CCCAGAAGCC AAAATGAGAG 2001 AGAGAGAGAG AGCACGCGTA CGTGCACCCT GGGGCAGTGT CTCACCGTAT 2051 GAATAAGGGA TGTAACACTA AAAGCCCATT AGGGGGCAGT GTTTCCCGCC 2101 TGTTGTAGAA ACTGGTACAG AAAGGATCCT ATATGAAGTT CCTGAAACTG 2151 ACCTTTGTCT ATTATTACCT TCTCTGAAAA GTGCCAGTCC ATGTATTTTT 2201 TATTTATTTT AAGTTTGTAA TTTAATTTTT AATTATTGTT TAGTGTTTGC 2251 ATTTAATTTT ATTTAATCAC CACATTTAGA AAATAATAAG AGCAAGTTTC 2301 TAAATGGGAG ACTGCTGAGG CTCTTTGCAA GAGATGAGAT TAAGTTTGAG 2351 TTTCTAAGGC AGGGCATGAG CTGGAAATAG CATTGCTTTC CTTGATTGTC 2401 TCTCTCCTTC AGGGAGATTC TTTTTCTCTA GTGTTTTAAG TGATCCTTTG 2451 AAGTAAGTGT GGAGAGTCTT GAATGGCAAG ACCAGGAGCT GAGTTTAAGC 2501 TTGTAATGGA AGCTTGCATT GTGGGATATA TAACTGAGGA AGCATATTTA 2551 TCCTGAAGGT ATTTTGCCAG AAGGTATCAC TTGACCTGGA AAAGGAATCT 2601 ATTTAGTTCA GGAAAGATAA AAAGTTTAGA GGTATGTGAA GGAAGCACTT 2651 AGAACTTGCA AGCCTGATGT CCTATCAAGT TATGTCTTCT GGGTGACAGA 2701 CAAAATAGCT TGTCTTATGG TGGTGATGTG TTGCATTTTC ACTTTGGGGT 2751 CTGTAAGAAA CTGTCAGTGA AAATATGTAC AATTCCTTCA ATTTCCATTC 2801 TTAACAACTG TAATGTTGAA AAATAAGTTG AAAAGTCTTT GGGACCATAC 2851 ATGCAAAAAC GGTGCCTCTG TTACTTAATT ATTTAATATT CTATAAATGT 2901 ACCCAATCTG TCCGCACCCT TCCCAGTGAT GGGGCAGTAT GTCTGAGGAA 2951 GTATAATTTC AGTACTGGGG TCGGGGAGAG GAGGTGATGT TTCTACATTT 3001 TTATTTTTTC TATAAATTGC AATTGGTCTG TATGCTGGTT TATTTTGAAA 3051 TTTATATTGG TTTCTTTTCA AGCTGGTGTC ATCTCCTAGA CTGTTTCACC 3101 CAGATGCTAG CATTTTTTTT TTTTTTGAGA CAGAGTCTCA CTCTGTCACC 3151 TAGGCTGGAG TTGCAGTGGT TTGATCTCGG CTCACTGCAA CCTCCGACTC 3201 CTGGGTTCAA GCAATTCTTC TGCCTCAGCC TCCTGAGTAG CTGGGATTAC 3251 AGATGTGCAC CAGCACACCC GGCTAATTTT TTGTATTTTT AGTAGAGACA 3301 GGGTTTCGCC ATGTTGGCCA GGCTGGTCTT GAACTCCTGG CCTTATGTGA 3351 TCCGCCCACC TTGGCTTCCC AAAGTGCTGG GATTACAGGC ATGAGCCACC 3401 TCGCCTGGCC AGATGCTAGC ATTTTAGATC AAACAATTCA TTTTAGATGA 3451 ATTGTTTTGT TTCACAATCA TTTTAAATCA TTTTAGAATG TACTTCACAT 3501 TATTAGTTGT GTTATGGCAT AAAGGTACAA CCATTCCCTA ACTCCATCTT 3551 TTATTAATGC TTAAGTTTAA ATTATATTCT TCCAATGCCT AAGCTATTCC 3601 CTAGAATTAA ACTGGGCACT TTTGGAAGCA GCAACAGTAA CAGCAGCAGC 3651 AAACTTTTCC TCTCATATTT TGGGTGTATC AAAAGTTCTA GACTTTTGAA 3701 GTTATGATTT CAGTGGCCCA CTTTATTTCT AAGGAAGAGT GTCTACTTTG 3751 GAACGATACT TTGCACATAG TAGGAACTCA AGAAATACAT TTGAATAATT 3801 ATAATTAACT GTTTAGCTAT CTTAATGAGA ATTTGTTGAC AACAAAAGAT 3851 CATCCATCGC CTTATGTGTG AGTAAGATTG GAGCCTCTAT CAAGATTTAG 3901 TCAAGTTCAG TTAGATTGAT TCTAGAAACA AATATTTATT TCTTTCTTTT 3951 ACGGGGATGT GAATAAGGCT TTTCCTTAAG GCCTTCATTC TTTAAACAAA 4001 CAGGTTGAAA TGGTATGTTG TAAAAGAGAA GACGGGAGAG AGGTATTTAG 4051 ATGATAAGTG TACTTCACAA AAATGCCAAA GTTTGAAAAA TAGGTATGTT 4101 TGTTCTAAAT GTTTAAGTGC TTCTCTGTTA GGTTCTGGGG CTTGCAATCA 4151 TTTGAATTGT TCTGTTTCAC AATAAAGGAG ATTCACTGGG TTCTGCATTT 4201 TCAGGATTCA ATAGAACTGC TCCATTAAAA AAATAATCCT TAGCAAGCAT 4251 TCGAATCCTA ACTGCTTTGA TGCACTTGCC CTCGGGCACC TGTCATTTCC 4301 AATATGGTAG GTGTCAAAGT CAAAAGTATT TACTGGGAGA AAAAAGAGAG 4351 GAGTGGTTGT AGAAGTCTCC CTAAATCAGA CATGTCAAGC AATCAGCCAA 4401 CGTGGTGTAT TTCTCATTCA ATATTTTAGT GTGAATTGAG ACACTGAGAT 4451 AAAGACATCG TGCAGAGATA AATGGGGATA CAGTTAAATG TAGCAACTCT 4501 TGAGTTCATT TTTTCCCACT GTAGCAAAAT TAATGCTTTC TCTTTATTGA 4551 AATAAATTGC TCATTCCTCC AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 4601 AAAAAAGG BLAST Results Entry HSG27587 from database EMBL: human STS SHGC-32548. Score = 1951, P = 9.0e-101, identities = 411/425 Entry HS073350 from database EMBL: human STS EST303564. Score = 1417, P = 8.7e-58, identities = 285/287 Medline entries No Medline entry Peptide information for frame 2 ORF from the beginning to 580 bp; peptide length: 194 Category: questionable ORF Classification: no clue BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_18f3, frame 2 PIR:CGB01S collagen alpha 1(1) chain - bovme (fragments), N = 1, Score = 155, P = 4.5e-10 TREMBL :HSCG1PA1_1 gene: "COL1A1"; Human proalpha 1 (I) chain of type I procollagen mRNA (partial)., N = 1, Score = 155, P = 6.5e-10 >PIR : CGB01S collagen alpha 1 ( 1 ) chain - bovine ( fragments ) Length = 779 HSPs : Score = 155 (23.3 bits), Expect = 4.5e-10, P = 4.5e-10 Identities = 60/152 (39%), Positives = 67/152 (44%) Query: 7 GEAGGPGAAWARRAAALPGTAA—GPPRPAAPPGA--APARGGPAPGAPAQALPRSQRGR 62 G+ G PG + AR PG GPP PA P GA AP G A A P SQ Sbjct: 230 GDLGAPGPSGARGERGFPGERGVEGPPGPAGPRGANGAPGNDGAKGDAGAPGAPGSQGAP 289 Query: 63 QLAERNGRPRRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGRHHHVRSLADLLQLPGAAE 122 L G P RGA PG GD +GA G + G VR L + PG A Sbjct: 290 GL QGMPGE-RGAAGLPGPKGDRGDAGPKGADGAPGKDG VRGLTGPIGPPGPAG 341 Query: 123 GAGDRGHL-P-GP DARDPELPRVFLPLAGLRGPPAA 156 GD+G P GP D +P P P AG GPP A Sbjct: 342 APGDKGEAGPSGPAGTRGAPGDRGEPGPPG P-AGFAGPPGA 381 Score = 121 (18.2 bits), Expect = 5.4e-05, P = 5.4e-05 Identities = 52/154 (33%), Positives = 60/154 (38%) Query: 7 GEAGGPGAAWARRAAALPGTAAGPPRPAAPPGAAPARG GPAPGAPAQALPRSQRG 61 G G PGAA R P AGPP P P G ++G GPA G P + P G Sbjct: 434 GATGFPGAA-GRVGPPGPSGNAGPPGPPGPAGKEGSKGPRGETGPA-GRPGEVGPPGPPG 491 Query: 62 RQLAERNGRPRRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGRHHHVRSLADLLQLPGAA 121 A G P G PG PG RG G +RG R L PG + Sbjct: 492 P—AGEKGAPGAD-GPAGAPGTPGPQGIAGQRGVVGLPGQRGE RGFPGL PGPS 541 Query: 122 EGAGDRGHLPGPDARDPELPRVFLPLAGLRGPPAAAVRE 160 G +G R P P + GL GPP + RE Sbjct: 542 GEPGKQGPSGASGERGPPGP MGPPGLAGPPGESGRE 577 Score = 117 (17.6 bits), Expect = 1.8e-04, P = 1.8e-04 Identities = 52/148 (35%), Positives = 62/148 (41%) Query: 7 GEAGGPGAAWARRAAALPGTAAGPPRPAA PPGAAPARGGPAPGAPAQALPRSQRG-R 62 G G PG AR +A PG A G P A PPG + GP PG P A +G R Sbjct: 416 GNVGAPGPKGARGSAGPPG-ATGFPGAAGRVGPPGPS-GNAGP-PGPPGPAGKEGSKGPR 472 Query: 63 QLAERNGRPRRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGRH--HHVRSLADLLQLPGA 120 GRP G + PG PG GA G G + ++ LPG Sbjct: 473 GETGPAGRP GEVGPPGPPGPAGEKGAPGADGPAGAPGTPGPQGIAGQRGVVGLPGQ 528 Query: 121 AEGAGDRGH--LPGPDARDPEL-PRVFLPLAGLRGPP 154 G+RG LPGP + P +G RGPP Sbjct: 529 R GERGFPGLPGPSGEPGKQGPS GASGERGPP 559 Score = 117 (17.6 bits), Expect = 1.8e-04, P = 1.8e-04 Identities = 54/162 (33%), Positives = 64/162 (39%) Query: 7 GEAGGPGAAWARRAAALPGT—AAGPPRPAAPPGAAPARG—GPA—PGAPAQALPRSQR 60 G G PG + PG A+GP P PPG G G A PG P + P + Sbjct: 29 GPPGAPGPQGFQGPPGEPGEPGASGPMGPRGPPGPPGKNGDDGEAGKPGRPGERGPPGPQ 88 Query: 61 G-RQLAERNGRP—RRHRGALAQPGHPGDLAAGVGRGAGGGHΞRRGRHHHV—RSLADLL 115 G R L G P + HRG G GD +G G G + R L Sbjct: 89 GARGLPGTAGLPGMKGHRGFSGLDGAKGDAGPAGPKGEPGSPGENGAPGQMGPRGLPGFP 148 Query: 116 QLPGAA--EG-AGDRGHLPGPDARDPELPRVFLPLAGLRGPPAAA 157 GAA G AG+RG +PGP P AG +GPP A Sbjct: 149 GPKGAAGEPGKAGERG-VPGPPGAVG--PAGKDGEAGAQGPPGPA 190 Score = 113 (17.0 bits), Expect = 5.4e-04, P = 5.4e-04 Identities = 54/148 (36%), Positives = 58/148 (39%) Query: 7 GEAGGPGAAWARRAAALPGTA AGPPRPAAP PGAAPARGGPAP-GAPAQALPR 57 G AG PGA A PG A AGPP PA P PG G P P GA A P Sbjct: 374 GFAGPPGADGQPGAKGEPGDAGAKGDAGPPGPAGPAGPPGPIGNVGAPGPKGARGSAGPP 433 Query: 58 ΞQRGRQLAERNGRPRRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGRHHHVRSLADLLQL 117 G A P G PG PG +G G GR V Sbjct: 434 GATGFPGAAGRVGPPGPSGNAGPPGPPGPAGKEGSKGPRGETGPAGRPGEVGP 486 Query: 118 PGAAEGAGDRGHLPGPD—ARDPELPRVFLPLAGLRG 152 PG AG++G PG D A P P +AG RG Sbjct: 487 PGPPGPAGEKG-APGADGPAGAPGTPGP-QGIAGQRG 521 Score = 110 (16.5 bits), Expect = 1.3e-03, P = 1.2e-03 Identities = 54/151 (35%), Positives = 60/151 (39%) Query: 7 GEAGGPGAAWARRAAALPGTAAGPPRPAAPPG—AAPAR-GGPAP-GAPAQALPRSQRGR 62 GE G G A + LPG A GPP A PG P G P P GA + +RG Sbjct: 194 GERGEQGPAGSPGFQGLPGPA-GPPGEAGKPGEQGVPGDLGAPGPSGARGERGFPGERGV 252 Query: 63 QLAERNGRPRRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGRHHHVRΞLADLLQLPGAAE 122 + PR GA G GD A G+ G +G R A L PG Sbjct: 253 EGPPGPAGPRGANGAPGNDGAKGDAGAPGAPGSQGAPGLQGMPGE-RGAAGL PGPK- 307 Query: 123 GAGDRGHLPGPDARD—PELPRVFLPLAGLRGPPAAA 157 GDRG GP D P V L G GPP A Sbjct: 308 —GDRGDA-GPKGADGAPGKDGV-RGLTGPIGPPGPA 340 Score = 109 (16.4 bits), Expect = 1.7e-03, P = 1.7e-03 Identities = 55/154 (35%), Positives = 60/154 (38%) Query: 4 NGN-GEAGGPGAAWARRAAALPGTAAGPPRPAAPPGAAPARG-GPAPGAPAQALPRSQRG 61 NG+ GEAG PG R P A G P A PG RG GA A P +G Sbjct: 67 NGDDGEAGKPGRP-GERGPPGPQGARGLPGTAGLPGMKGHRGFSGLDGAKGDAGPAGPKG 125 Query: 62 RQLAE-RNGRPRRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGRHHHVRΞL ADLL 115 + NG P + G PG PG A G G G V A Sbjct: 126 EPGSPGENGAPGQ-MGPRGLPGFPGPKGAAGEPGKAGERGVPGPPGAVGPAGKDGEAGAQ 184 Query: 116 QLPGAAEGAGDRGHLPGPDARDPELPRVFLPLAGLRGPPAAA 157 PG A AG+RG GP A P F L G GPP A Sbjct: 185 GPPGPAGPAGERGE-QGP-AGΞPG FQGLPGPAGPPGEA 220 Score = 104 (15.6 bits), Expect = 6.6e-03, P = 6.6e-03 Identities = 44/131 (33%), Positives = 49/131 (37%) Query: 2 EVNGNGEAGGPGAAWARRAAALPGTAAGPPRPAAPPGAAPARGGPAP-GAPAQALPRSQR 60 E GE G PG R LPG GP A PG A RG P P GA A + Sbjct: 126 EPGSPGENGAPGQMGPR GLPGFP-GPKGAAGEPGKAGERGVPGPPGAVGPAGKDGEA 181 Query: 61 GRQLAERNGRPRRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGRHHHVRSLADLLQLPGA 120 G Q P RG G PG G+ G G G+ DL PG Sbjct: 182 GAQGPPGPAGPAGERGEQGPAGSPG--FQGLP-GPAGPPGEAGKPGEQGVPGDL-GAPGP 237 Query: 121 AEGAGDRGHLPG 132 + G+RG PG Sbjct: 238 SGARGERG-FPG 248 Score = 104 (15.6 bits), Expect = 6.6e-03, P = 6.6e-03 Identities = 43/131 (32%), Positives = 55/131 (41%) Query: 7 GEAGGPGAAWARRAAALPGTAAGPPRPAAPPGAAPARGGPAPGAPAQALPRSQRGRQLAE 66 GEAG G A R A PG G P P P G A GP PGA Q + + G A+ Sbjct: 347 GEAGPSGPAGTRGA PGDR-GEPGPPGPAGFA GP-PGADGQPGAKGEPGDAGAK 397 Query: 67 RNGRPRRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGRHHHVRSLADLLQLPGAAEGAGD 126 + P G PG G++ A +GA G G + A + PG + AG Sbjct: 398 GDAGPPGPAGPAGPPGPIGNVGAPGPKGARGSAGPPGATGFPGA-AGRVGPPGPΞGNAGP 456 Query: 127 RGHLPGPDARD 137 G PGP ++ Sbjct: 457 PGP-PGPAGKE 466 Score = 104 (15.6 bits). Expect = 6.6e-03, P = 6.6e-03 Identities = 56/162 (34%), Positives = 62/162 (38%) Query: 7 GEAGGPGAAWARRAAALPGTAA—GPPRPAAPPGAAPARGGPAPGAPAQALPRSQRGRQL 64 G G PGA A G GP P P G A ARG P P Q PR +G Sbjct: 608 GPPGAPGAPGPVGPAGKSGDRGETGPAGPIGPVGPAGARG PAGP-QG-PRGBKGZTG 662 Query: 65 AERNGRPRRHRG ALAQPGHPGDLAAGVGRGAGGGHSRRGRHHHVRSLA-DLLQ-LPG 119 + + + HRG PG PG GA G RG S D L LPG Sbjct: 663 ZZGBRGIKGHRGFSGLQGPPGPPGSPGEQGPSGASGPAGPRGPPGSAGSPGKDGLNGLPG 722 Query: 120 AAEGAGDRGHL—PGPDARDPELPRVFLPLAGLRGPPAAAVREERLHRPVQ 168 G RG GP A P P P G GPP+ L +P Q Sbjct: 723 PIGPPGPRGRTGDAGP-AGPPGPPG P-PGPPGPPSGGYDLSFLPQPPQ 768 Score = 101 (15.2 bits), Expect = 1.5e-02, P = 1.5e-02 Identities = 49/148 (33%), Positives = 55/148 (37%) Query: 7 GEAGGPGAAWARRAAALPGTAAGPPRPAAPPGAAPARGGPAPGAPA QALPRSQRGR 62 G AG PG A R PG A GP A G A A+G P P PA + P G Sbjct: 152 GAAGEPGKAGERGVPGPPG-AVGP AGKDGEAGAQGPPGPAGPAGERGEQGPAGSPGF 207 Query: 63 QLAERNGRPRRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGRHHHVRSLADLLQLPGAAE 122 Q P G + G PGDL A G G RG R + PG A Sbjct: 208 QGLPGPAGPPGEAGKPGEQGVPGDLGAP GPSGARGERGFPGE-RGVEGP PGPAG 260 Query: 123 GAGDRGHLPGPDARDPELPRVFLPLAGLRGPP 154 G G PG D + P G +G P Sbjct: 261 PRGANG-APGNDGAKGDAGAPGAP—GSQGAP 289 Score = 100 (15.0 bits), Expect = 1.9e-02, P = 1.9e-02 Identities = 40/130 (30%), Positives = 48/130 (36%) Query: 7 GEAGGPGAAWARRAAALPG —AAGPPRPAAPPGAAPARG—GPA—PGAPAQALPRSQR 60 G G PG + PG A+GP P PPG G G A PG P + P + Sbjct: 29 GPPGAPGPQGFQGPPGEPGEPGASGPMGPRGPPGPPGKNGDDGEAGKPGRPGERGPPGPQ 88 Query: 61 G-RQLAERNGRP--RRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGRHHHVRSLADLLQL 117 G R L G P + HRG G GD +G G G + L Sbjct: 89 GARGLPGTAGLPGMKGHRGFΞGLDGAKGDAGPAGPKGEPGSPGENGAPGQMGPRG-LPGF 147 Query: 118 PGAAEGAGDRG 128 PG AG+ G Sbjct: 148 PGPKGAAGEPG 158 Score = 99 (14.9 bits), Expect = 2.5e-02, P = 2.5e-02 Identities = 53/156 (33%), Positives = 61/156 (39%) Query: 7 GEAGGPGAAWARRA AALPGT—AAGPPRPAAPPGAAPARG—GPA PGAPAQAL 55 G G PGA R A PG A G P P P G + RG GPA P PA A Sbjct: 587 GRDGSPGAKGDRGETGPAGAPGPPGAPGAPGPVGPAGKSGDRGETGPAGPIGPVGPAGAR 646 Query: 56 PRSQRGRQLAERNGRPRRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGRHHHV 108 PR +G + + + HRG G PG + +G G G Sbjct: 647 GPAGPQGPRGBKGZTGZZGBRGIKGHRGFSGLQGPPGPPGSPGEQGPSGASGPAGPRGP- 705 Query: 109 RΞLADLLQLPGAAEGAGDRG—HLPGPDARDPELPRVFLPLAGLRGPP 154 PG+A G G LPGP P PR AG GPP Sbjct: 706 PGSAGSPGKDGLNGLPGPIG—PPGPRGRTGDAGPAGPP 742 Score = 98 (14.7 bits), Expect = 3.3e-02, P = 3.3e-02 Identities = 51/158 (32%), Positives = 58/158 (36%) Query: 7 GEAGGPGAAWARRAAALPGTA AGPPRPAAPPGAAPARGGPAP-GAPAQALPRSQR 60 G G G R AA LPG AGP PG RG P G P A + Sbjct: 287 GAPGLQGMPGERGAAGLPGPKGDRGDAGPKGADGAPGKDGVRGLTGPIGPPGPAGAPGDK 346 Query: 61 GRQLAERNGRPRRHRGA LAQPGHPGDLAAGVGRGAGGGHSRRGRHHHVRSLADLLQL 117 G A +G P RGA +PG PG GA G +G + D Sbjct: 347 GE—AGPSG-PAGTRGAPGDRGEPGPPGPAGFAGPPGADGQPGAKGEPGDAGAKGDAGP- 402 Query: 118 PGAAEGAGDRGHLPGPDARDPELPRVFLPLAGLRGPPAAAVR 159 PG A AG G + A P+ R G G P AA R Sbjct: 403 PGPAGPAGPPGPIGNVGAPGPKGARGSAGPPGATGFPGAAGR 444 Score = 96 (14.4 bits), Expect = 5.7e-02, P = 5.5e-02 Identities = 46/152 (30%), Positives = 57/152 (37%) Query: 6 NGEAGGPGAAWARRAAALPGTAA—GPPRPAAPPGAAPARGGPAPGAPA-QALPRΞQRGR 62 +G G PGA + PG G PA PG A G P P PA ++ R + G Sbjct: 574 SGREGAPGAEGSPGRDGSPGAKGDRGETGPAGAPGPPGAPGAPGPVGPAGKSGDRGETGP 633 Query: 63 QLAERNGRPRRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGRHHHVRSLADLLQLPGAAE 122 P RG G G+ +G G RG H R + L PG Sbjct: 634 AGPIGPVGPAGARGPAGPQGPRGB KGZTGZZGBRGIKGH-RGFΞGLQGPPGPPG 686 Query: 123 GAGDRGHLPGPDARDPELPRVFLPLAGLRGPPAAA 157 G++G P A P AG RGPP +A Sbjct: 687 SPGEQG--PS-GASGP AGPRGPPGSA 709 Score = 94 (14.1 bits), Expect = 9.7e-02, P = 9.2e-02 Identities = 45/134 (33%), Positives = 56/134 (41%) Query: 24 PGTAAGPPRPAAPPGAAPARGGPA-PGAPAQALPRSQRGRQLAERNGRPRRHR—GALAQ 80 P G P P PG +G P PG P + P RG G P ++ G + Sbjct: 21 PSGPRGLPGPPGAPGPQGFQGPPGEPGEPGASGPMGPRGPP GPPGKNGDDGEAGK 75 Query: 81 PGHPGDLAA-GV—GRGAGGGHSRRGRHHHVRSLADLLQLPGAAEGAGDRGH—LPGPDA 135 PG PG+ G RG G G H R + L G A AG +G PG + Sbjct: 76 PGRPGERGPPGPQGARGLPGTAGLPGMKGH-RGFSGLDGAKGDAGPAGPKGEPGSPGENG 134 Query: 136 RDPEL-PRVFLPLAGLRGPPAAA 157 ++ PR LP G GP AA Sbjct: 135 APGQMGPRG-LP—GFPGPKGAA 154 Score = 92 (13.8 bits), Expect = 1.7e-01, P = 1.5e-01 Identities = 52/155 (33%), Positives = 58/155 (37%) Query: 7 GEAGGPGAAWARRAAALPGTAAGPPRPAAPPGAAPARGGP-APGAPAQALPRΞQRGRQLA 65 GEAG G A R A G GPP PA G A G P A G P A + G Sbjct: 347 GEAGPSGPAGTRGAPGDRGEP-GPPGPAGFAGPPGADGQPGAKGEPGDAGAKGDAGPPGP 405 Query: 66 ERNGRPRRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGR--HHHVRSLADLLQLPGAA— 121 P G + PG G + GA G GR A PG A Sbjct: 406 AGPAGPPGPIGNVGAPGPKGARGSAGPPGATGFPGAAGRVGPPGPSGNAGPPGPPGPAGK 465 Query: 122 EGA-GDRGHLPGPDARDPELPRVFLP-LAGLRGPPAA 156 EG+ G RG GP R E+ P AG +G P A Sbjct: 466 EGSKGPRGET-GPAGRPGEVGPPGPPGPAGEKGAPGA 501 Score = 92 (13.8 bits), Expect = 1.7e-01, P = 1.5e-01 Identities = 51/156 (32%), Positives = 57/156 (36%) Query: 7 GEAGGPGAAWARRA AALPGT—AAGPPRPAAPPGAAPARGGPAPGAPAQAL-PRSQR 60 G G PGA R A PG A G P P P G + RG P P + P R Sbjct: 587 GRDGSPGAKGDRGETGPAGAPGPPGAPGAPGPVGPAGKSGDRGETGPAGPIGPVGPAGAR 646 Query: 61 GRQLAERNGRPRRHRGALAQPGHPGDLA-AGVG—RGAGGGHSRRGRH—HHVRSLADLL 115 G A G PR +G + G G G +G G G A Sbjct: 647 GP--AGPQG-PRGBKGZTGZZGBRGIKGHRGFSGLQGPPGPPGSPGEQGPSGASGPAGPR 703 Query: 116 QLPGAAEGAGDRG—HLPGPDARDPELPRVFLPLAGLRGPP 154 PG+A G G LPGP P PR AG GPP Sbjct: 704 GPPGSAGSPGKDGLNGLPGPIG—PPGPRGRTGDAGPAGPP 742 Score = 90 (13.5 bits), Expect = 2.8e-01, P = 2.5e-01 Identities = 45/134 (33%), Positives = 53/134 (39%) Query: 7 GEAGGPGAAWARRAAALPGTAAGPPRPAAPPGAAPARGGPAPGAPAQALPRSQRGRQ-LA 65 G G PG A + A G A P P P G A RG G P Q R +RG L Sbjct: 485 GPPGPPGPAGEKGAPGADGPAGAPGTPG-PQGIAGQRG--VVGLPGQ RGERGFPGLP 538 Query: 66 ERNGRPRRH--RGALAQPGHPGDLA AGV GR-GAGGGHSRRGRHHHVRSLADL 114 +G P + GA + G PG + AG GR GA G GR + D Sbjct: 539 GPSGEPGKQGPSGASGERGPPGPMGPPGLAGPPGESGREGAPGAEGSPGRDGSPGAKGDR 598 Query: 115 LQL-PGAAEGAGDRGHLPGP 133 + P A G PGP Sbjct: 599 GETGPAGAPGPPGAPGAPGP 618 Score = 83 (12.5 bits), Expect = 1.8e+00, P = 8. 3 e-01 Identities = 49/156 (31%), Positives = 56/156 (35%) Query: 7 GEAGGPGAAWARRAAALPGTAA—GPPRPAAPPGAAPARG—GPAP—GAPAQALPRSQR 60 G+AG GA A + G GPP PA PG G GPA GAP R + Sbjct: 311 GDAGPKGADGAPGKDGVRGLTGPIGPPGPAGAPGDKGEAGPSGPAGTRGAPGD RGEP 367 Query: 61 GRQLAERNGRPRRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGRHHHVRSLADLLQLPGA 120 G P G G PGD A G G G + ++ PG Sbjct: 368 GPPGPAGFAGPPGADGQPGAKGEPGDAGAKGDAGPPGPAGPAGPPGPIGNVG APGP 423 Query: 121 AEGAGDRGHLPGPDARDPELPRVFLP LAGLRGPPAAAVRE 160 G G PG RV P AG GPP A +E Sbjct: 424 KGARGSAGP-PGATGFPGAAGRVGPPGPSGNAGPPGPPGPAGKE 466 Score = 82 (12.3 bits). Expect = 2.3e+00, P = 9.0e-01 Identities = 46/148 (31%), Positives = 52/148 (35%) Query: 7 GEAGGPGAAWARRAAALPGTAAGPPRPAAPPGAAPARGGPAPGAPAQALPRSQRGRQLAE 66 G+AG PGA ++ A L G G A PG RG P A P R L Sbjct: 275 GDAGAPGAPGSQGAPGLQGMP-GERGAAGLPGPKGDRGDAGPKG-ADGAPGKDGVRGLTG 332 Query: 67 RNGRPRRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGRHHHVRSLADLLQLPGAAEGAGD 126 G P G PG G+ G G RG A PGA G Sbjct: 333 PIGPP GPAGAPGDKGEAGPSGPAGTRGAPGDRGEPGPPGP-AGFAGPPGADGQPGA 387 Query: 127 RGHLPGP-DARDPELPRVFLPLAGLRGPP 154 +G PG A+ P P AG GPP Sbjct: 388 KGE-PGDAGAKGDAGPPG—P-AGPAGPP 412 Peptide information for frame 3 ORF from 12 bp to 755 bp; peptide length: 248 Category: similarity to known protein Classification: unset Prosite motifs: LEUCINE_ZIPPER (17-39) LEUCINE ZIPPER (24-46) BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_18f3, frame 3 TREMBL :AF070675_1 product: "TNF-inducible protein CG12-1"; Homo sapiens TNF-inducible protein CG12-1 mRNA, complete eds., N = 1, Score = 135, P = le-06 TREMBL :HS6802_1 gene: "dJ6802.1"; product: "dJ6802.1"; Homo sapiens DNA sequence from PAC 6802 on chromosome 22. Contains apolipoprotein L, myosin heavy chain, ESTs, CA repeat, STS and GSS., N = 1, Score = 107, P = 0.0023 >TREMBL:AF070675_1 product: "TNF-inducible protein CG12-1"; Homo sapiens TNF-mducible protein CG12-1 mRNA, complete eds. Length = 331 HSPs: Score = 135 (20.3 bits), Expect = 1.0e-06, P = 1.0e-06 Identities = 30/103 (29%), Positives = 55/103 (53%) Query: 30 RLHRQVLRLREVARRLERLRRRSLVANVAGSSLSATGALAAIVGLSLSPVTLGTSLLVSA 89 ++ + +LR +A +E + R ++NV SS A + ++ GL L+P T GTSL ++A Sbjct: 91 KIQESIEKLRALANGIEEVHRGCTISNVVSSSTGAASGIMSLAGLVLAPFTAGTSLALTA 150 Query: 90 VGLGVATAGGAVTITSDL-SLIFCNSRELRRVQEIAATCQDQMR 132 G+G+ A IT+ + + +S E + AT D+++ Sbjct: 151 AGVGLGAASAVTGITTSIVEHSYTSSAEAE-ASRLTATSIDRLK 193 Pedant information for DKFZphtes3_18f3, frame 2 Report for DKFZphtes3_18f3.2 [LENGTH] 193 [MW] 19708.24 [pi] 11.90 [KW] All_Alpha [KW] LOW_COMPLEXITY 55.44 % SEQ TEVNGNGEAGGPGAAWARRAAALPGTAAGPPRPAAPPGAAPARGGPAPGAPAQALPRSQR SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx... PRD cccccccccccccchhhhhhhhhccccccccccccccccccccccccccccccchhhhhh SEQ GRQLAERNGRPRRHRGALAQPGHPGDLAAGVGRGAGGGHSRRGRHHHVRSLADLLQLPGA SEG xxxxxxxxxxxxxxxxxxxxxx PRD hhhhhhcccccccccccccccccccccccccccccccccccccccchhhhhhhhhccccc SEQ AEGAGDRGHLPGPDARDPELPRVFLPLAGLRGPPAAAVREERLHRPVQFCLLHRLLWLTW SEG xxxxxxxxxxxxx xxxxxxxxxx PRD ccccccccccccccccccccccccccccccccccchhhhhhhhcccchhhhhhhhhhhhc SEQ LPHPQAGGGGHQG SEG xxxxxxxxxxxxx PRD ccccccccccccc (No Prosite data available for DKFZphtes3_18f3.2) (No Pfam data available for DKFZphtes3_18f3.2) Pedant information for DKFZphtes3_18f3, frame 3 Report for DKFZphtes3_18 f 3 . 3 [LENGTH] 248 [MW] 27162.56 [pi] 9.92 [PROSITE] LEUCINE ZIPPER 2 [KW] TRANSMEMBRANE 1 [KW] LOW COMPLEXITY 30.65 % [KW] COILED COIL 12.10 % SEQ MGMERPAAREPHGPDALRRFQGLLLDRRGRLHRQVLRLREVARRLERLRRRΞLVANVAGS SEG XXXXXXXXXXXXXXXXXX . XXXXXXXXXXXXXXXXXXXX . . XXX PRD cccccccccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccc COILS MEM SEQ SLSATGALAAIVGLSLΞPVTLGTSLLVΞAVGLGVATAGGAVTITSDLSLIFCNSRELRRV SEG xxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxx PRD cchhhhhhhhhhhhcccccccccccccccccceeeeccceeeeeeceeeeecchhhhhhh COILS MEM MMMMMMMMMMMMMMMMM SEQ QEIAATCQDQMREILSCLEFFCRWQGCGDRQLLQCGRNASIALYNSVYFIVFFGSRGFLI SEG PRD hhhhhhhhhhhhhhhhhhhhhhhcccccchhhhhccccchhhhhcceeeeeecccccccc COILS MEM SEQ PRRAEGDTKVSQAVLKAKIQKLAESLESCTGALDELSEQLESRVQLCTKSSRGHDLKISA SEG PRD ccccccccchhhhhhhhhhhhhhhhhhhhhcchhhhhhhhhhhhhhhhcccccceeeehh COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCC MEM SEQ DQRAGLFF SEG PRD hhhhhccc COILS MEM Prosite for DKFZphtes3_18f3.3 PS00029 17->39 LEUCINE_ZIPPER PDOC00029 PS00029 24->46 LEUCINE ZIPPER PDOC00029 (No Pfam data available for DKFZphtes3_18f3.3) DKFZphtes3_1817 group: cell structure and motility DKFZphtes3_1817 encodes a novel 1050 amino acid protein with weak partial similarity to ankyrins . The novel protein contains an ATP/GTP-bindmg site motif A (P-loop) and an Ank repeat. Ankyrins are peripheral membrane proteins which interconnect integral proteins with the spectrin-based membrane skeleton. Thus the novel protein seems to be involved in coupling of cyto skeleton and cell membrane. The new protein can find application in modulation of cyto skeleton-membrane interactions. similarity to ankyrins Sequenced by MediGenomix Locus : unknown Insert length: 4501 bp Poly A stretch at pos. 4423, no polyadenylation signal found 1 GATCGCCGCG CGAGGGTGGT GGGCATCGAG GTCCCAGCAG CGGACGAGGG 51 AGGTGCCGCC GTCGCCCAGG ATGGGCTGGG AATGAAGCGA TGTAGCCTTT 101 TAAGAGATTT GCTCTGACCC ATCTGAAGTC CATATGGCTC TGTATGATGA 151 AGACCTCCTG AAAAATCCTT TCTATCTGGC TCTGCAAAAG TGCCGCCCTG 201 ACTTGTGCAG CAAAGTGGCC CAAATCCATG GCATTGTCTT AGTACCCTGC 251 AAAGGAAGCC TGTCGAGCAG CATCCAGTCT ACTTGTCAGT TTGAGTCCTA 301 CATTTTGATA CCTGTGGAAG AGCATTTTCA GACCTTAAAT GGAAAGGATG 351 TCTTTATTCA AGGGAACAGG ATTAAATTAG GAGCTGGTTT TGCCTGTCTT 401 CTCTCAGTGC CCATTCTCTT TGAAGAAACT TTCTACAATG AAAAAGAAGA 451 GAGTTTCAGC ATCCTGTGTA TAGCCCATCC TTTGGAAAAG AGAGAGAGTT 501 CAGAAGAGCC TTTGGCACCC TCAGATCCCT TTTCCCTGAA AACCATTGAA 551 GATGTGAGAG AGTTCTTGGG AAGACACTCC GAGCGATTTG ACAGGAACAT 601 CGCCTCTTTC CATCGAACAT TCCGAGAATG CGAGAGAAAG AGCCTCCGTC 651 ACCACATAGA CTCAGCGAAT GCTCTCTACA CCAAATGCCT CCAGCAGCTT 701 CTGAGGGACT CTCACCTGAA AATGCTCGCC AAGCAGGAGG CCCAGATGAA 751 CCTGATGAAG CAGGCAGTGG AGATATACGT CCATCATGAA ATTTACAACC 801 TGATCTTTAA ATACGTGGGG ACCATGGAGG CAAGTGAGGA TGCGGCCTTT 851 AACAAAATCA CAAGAAGCCT TCAAGATCTT CAGCAGAAAG ATATTGGTGT 901 GAAACCGGAG TTCAGCTTTA ACATACCTCG TGCCAAAAGA GAGCTGGCTC 951 AGCTGAACAA ATGCACCTCC CCACAGCAGA AGCTTGTCTG CTTGCGAAAA 1001 GTGGTGCAGC TCATTACACA GTCTCCAAGC CAGAGAGTGA ACCTGGAGAC 1051 CATGTGTGCT GATGATCTGC TATCAGTCCT GTTATACTTG CTTGTGAAAA 1101 CGGAGATCCC TAATTGGATG GCAAATTTGA GTTACATCAA AAACTTCAGG 1151 TTTAGCAGCT TGGCAAAGGA TGAACTGGGA TACTGCCTGA CCTCATTCGA 1201 AGCTGCCATT GAATATATTC GGCAAGGAAG CCTCTCTGCT AAACCCCCTG 1251 AGTCTGAGGG ATTTGGAGAC AGGCTGTTCC TTAAGCAGAG AATGAGCTTA 1301 CTCTCTCAGA TGACTTCGTC TCCCACCGAC TGCCTGTTTA AGCACATTGC 1351 ATCAGGTAAC CAGAAAGAAG TGGAGAGACT TCTGAGCCAA GAGGACCATG 1401 ATAAAGATAC CGTCCAAAAG ATGTGTCACC CTCTCTGCTT CTGCGATGAC 1451 TGTGAGAAAC TCGTCTCTGG GAGGTTGAAT GATCCCTCAG TTGTCACTCC 1501 ATTCTCCAGA GACGACAGGG GGCACACCCC TCTCCATGTG GCTGCTGTCT 1551 GTGGGCAGGC ATCCCTCATC GACCTCCTGG TTTCCAAGGG CGCCATGGTA 1601 AATGCCACAG ACTACCATGG GGCCACTCCG CTCCACCTGG CCTGTCAGAA 1651 GGGCTACCAG AGCGTGACGC TGCTGCTGCT GCACTACAAG GCCAGCGCGG 1701 AAGTGCAGGA CAACAATGGG AATACGCCAC TCCACCTGGC CTGCACCTAC 1751 GGCCACGAGG ACTGTGTGAA GGCTCTGGTT TACTACGACG TGGAGTCGTG 1801 CAGACTTGAC ATTGGCAATG AGAAAGGAGA CACCCCTCTA CACATTGCTG 1851 CCCGCTGGGG CTACCAAGGC GTCATAGAGA CATTGCTGCA GAACGGAGCG 1901 TCCACCGAGA TCCAGAACAG ACTGAAGGAG ACGCCCCTCA AGTGTGCATT 1951 AAACTCAAAG ATTCTGTCTG TAATGGAAGC CTATCACCTG TCCTTCGAGA 2001 GGAGGCAGAA GTCGTCCGAG GCCCCTGTGC AGTCCCCGCA GCGCTCCGTG 2051 GACTCCATCA GCCAAGAGTC CTCCACTTCC AGCTTCTCCT CCATGTCAGC 2101 CGGCTCAAGG CAGGAGGAGA CCAAGAAGGA CTACAGAGAG GTAGAAAAAC 2151 TTTTGAGAGC AGTTGCTGAT GGAGATCTAG AAATGGTGCG TTACCTGTTG 2201 GAATGGACAG AGGAGGACCT GGAGGATGCG GAGGACACTG TCAGTGCAGC 2251 AGACCCCGAA TTCTGTCACC CGTTGTGCCA GTGCCCCAAG TGTGCCCCAG 2301 CTCAGAAGAG GCTGGCGAAG GTTCCTGCCA GTGGGCTTGG TGTGAACGTG 2351 ACCAGCCAGG ACGGCTCCTC CCCGCTGCAT GTCGCCGCCC TGCACGGCCG 2401 GGCGGACCTC ATCCGCCTCC TGCTGAAGCA CGGGGCCAAC GCAGGTGCCA 2451 GGAACGCAGA CCAAGCCGTC CCGCTCCACC TGGCCTGCCA GCAGGGCCAC 2501 TTTCAGGTGG TGAAGTGTCT GTTAGATTCG AATGCAAAAC CCAATAAGAA 2551 GGACCTCAGT GGAAACACGC CCCTCATTTA CGCCTGCTCC GGTGGCCATC 2601 ACGAGCTTGT GGCACTGCTG CTACAGCACG GGGCCTCCAT TAACGCTTCT 2651 AACAATAAGG GCAACACAGC GCTGCACGAG GCTGTGATTG AAAAGCACGT 2701 CTTCGTGGTA GAGCTGCTTC TGCTCCACGG AGCGTCAGTT CAGGTGCTGA 2751 ACAAGCGGCA GCGCACGGCT GTAGACTGTG CTGAACAGAA TTCAAAAATA 2801 ATGGAATTGC TTCAGGTGGT ACCAAGCTGT GTTGCTTCAT TAGATGATGT 2851 GGCTGAAACT GACCGCAAGG AGTATGTCAC TGTTAAGATC AGGAAAAAAT 2901 GGAACTCAAA ACTGTATGAT CTACCAGATG AGCCTTTTAC AAGACAGTTT 2951 TACTTTGTCC ACTCAGCTGG TCAGTTTAAG GGAAAGACTT CAAGGGAGAT 3001 TATGGCAAGA GATAGAAGTG TCCCTAATTT AACCGAAGGT TCTTTGCATG 3051 AGCCAGGGAG GCAAAGTGTC ACACTGAGAC AGAATAACCT GCCAGCTCAG 3101 AGTGGATCTC ATGCTGCTGA GAAAGGCAAC AGCGACTGGC CAGAGAGGCC 3151 TGGACTGACA CAGACTGGCC CTGGACACAG ACGGATGCTG CGGAGACACA 3201 CGGTAGAGGA TGCGGTCGTG TCCCAGGGCC CGGAGGCTGC TGGCCCCCTC 3251 TCCACTCCCC AAGAGGTTAG TGCTTCCCGG TCCTAACAGG AATGAGGAGT 3301 TGTTGAACCC ACTGCTAGGA AGCAAGGATG CAACAAGATG ATGCTGAGCG 3351 TGAACACATC TGAGAACTAA ATGTGCTTCC ATGAGACTGG CTTGAGAAGT 3401 CTTCAGCACC AAGTTCCTGA AAGCTTTTCT GTGGCAGGAA AGAATGCAAC 3451 AAAAAAGTTA ACCACCACCA TCTCTCTCCT CTTCAAAGCT AATGAATACA 3501 ATTGAAACAG ACAAAAATTC CAGTAGCATC CAGATCCTTA AGCCAGAGGT 3551 GCATGCTTCT TTTTAAGTAT GAGGGTTTGT TGGTCACAGT GGGAGAGGTT 3601 TCACCACCGC ATTCTGACCT CCTCCTCCCA AAAGGTGCTA AACCTCTCTG 3651 ACCTGTGTAC ATTCACAAAC CACAGCTAGA ATTCCTCCAC CTAGGATTAA 3701 GCTGGAGAGA AGTAAGTAAT TTAGGTTTCA TGGTACTGTA GAGGCCAGGC 3751 TGAAATGTCA TATCTGAAGG AAGAAAGCAG CAGCTGGACA ATGTTTCTTT 3801 GCAAAGCAAC ACTCGAACCA AAAGATGCCT CAATCCCATT TTGATATTCA 3851 TTTTAGTGAA AGGATGCATC AGACCTGTTC CACATCATGC ACATGGGAAA 3901 GGGTGGTTAT CATTTTCCTT CTAACAAGTA GGTACAGATA TTCGGTTACT 3951 ACACGTGCAC CTGTAGCAGT ATTTCTAGAA ACATCCCTTT TTGTTGAGAA 4001 CCTCCCTTGA ATGTCTGTCA CACTCACACC TGACGGGATG GTTACTGGAT 4051 TAGAGAGTAG ATTTGGCACA TCTTTTCTTA GTCTTTTGAT TCAAATTCAA 4101 AACTTAACAG CACAAACCAG GTCAGAGTTA CTTTCGGTTA GAATTTATTG 4151 CCATTTATTC CTTTTTATAA ATTTCTATAG ATTATACTGT TATTTTTATG 4201 TTATTGGCCT AGAGCTACAC GTATATGGGT TTGTCCTGAG TCCGTTTTCA 4251 AATGACCTTG TGATAGGGAA ATGGTTTTGT CCATGTTCTT GGAAATACTT 4301 GTGTATGTAC AGAAGGAAGG GAGGGATTAT TTTTCTACAA AGTAATTTAT 4351 GATTTCTAAT TTTCTAATGT GCCTTGGATA TGTGCCAAAT GATGGAAAAG 4401 AAACAGTAAA CTTTATGATT CTTAAAAAAA AAAAAAAAAA AAAAAAAAAA 4451 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAG 4501 G BLAST Results No BLAST result • Medline entries No Medlme entry Peptide information for frame 2 ORF from 134 bp to 3283 bp; peptide length: 1050 Category: similarity to known protein Classification: Cell structure/motility Prosite motifs: ATP GTP A (945-953) 1 MALYDEDLLK NPFYLALQKC RPDLCSKVAQ IHGIVLVPCK GSLSSSIQST 51 CQFESYILIP VEEHFQTLNG KDVFIQGNRI KLGAGFACLL SVPILFEETF 101 YNEKEESFSI LCIAHPLEKR ESSEEPLAPS DPFSLKTIED VREFLGRHSE 151 RFDRNIASFH RTFRECERKS LRHHIDSANA LYTKCLQQLL RDSHLKMLAK 201 QEAQMNLMKQ AVEIYVHHEI YNLIFKYVGT MEASEDAAFN KITRSLQDLQ 251 QKDIGVKPEF SFNIPRAKRE LAQLNKCTSP QQKLVCLRKV VQLITQSPSQ 301 RVNLETMCAD DLLSVLLYLL VKTEIPNWMA NLSYIKNFRF ΞSLAKDELGY 351 CLTSFEAAIE YIRQGSLSAK PPESEGFGDR LFLKQRMSLL SQMTSSPTDC 401 LFKHIASGNQ KEVERLLSQE DHDKDTVQKM CHPLCFCDDC EKLVSGRLND 451 PSVVTPFSRD DRGHTPLHVA AVCGQASLID LLVΞKGAMVN ATDYHGATPL 501 HLACQKGYQS VTLLLLHYKA SAEVQDNNGN TPLHLACTYG HEDCVKALVY 551 YDVESCRLDI GNEKGDTPLH IAARWGYQGV IETLLQNGAS TEIQNRLKET 601 PLKCALNSKI LSVMEAYHLS FERRQKSSEA PVQSPQRSVD SISQESSTSS 651 FSSMSAGSRQ EETKKDYREV EKLLRAVADG DLEMVRYLLE WTEEDLEDAE 701 DTVSAADPEF CHPLCQCPKC APAQKRLAKV PASGLGVNVT SQDGSSPLHV 751 AALHGRADLI RLLLKHGANA GARNADQAVP LHLACQQGHF QVVKCLLDSN 801 AKPNKKDLSG NTPLIYACSG GHHELVALLL QHGASINASN NKGNTALHEA 851 VIEKHVFVVE LLLLHGASVQ VLNKRQRTAV DCAEQNSKIM ELLQVVPSCV 901 ASLDDVAETD RKEYVTVKIR KKWNSKLYDL PDEPFTRQFY FVHSAGQFKG 951 KTSREIMARD RSVPNLTEGS LHEPGRQSVT LRQNNLPAQS GSHAAEKGNS 1001 DWPERPGLTQ TGPGHRRMLR RHTVEDAVVΞ QGPEAAGPLS TPQEVSASRS BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_1817, frame 2 TREMBL:HSU43965_1 gene: "ANK3"; product: "ankyrin G119"; Human ankyrin G119 (ANK3) mRNA, complete ds., N = 2, Score = 287, P = 3.7e-21 PIR:I49502 ankyrin - mouse, N = 3, Score = 365, P = 2.2e-27 TREMBL : HSANKY_2 product: "alt. ankyrin (variant 2.2)"; Human mRNA for ankyrin (variant 2.1), N = 2, Score = 380, P = 7.3e-31 SWISSPROT :ANK1_HUMAN ANKYRIN R (ANKYRINS 2.1 AND 2.2) (ERYTHROCYTE ANKYRIN)., N = 2, Score = 380, P = 8.2e-31 PIR:SJHUK ankyrin 1, erythrocyte splice form 1 - human, N = 2, Score = 380, P = 8.2e-31 >TREMBL : HSANKY_2 product: "alt. ankyrin (variant 2.2)"; Human mRNA for ankyrin (variant 2.1) Length = 1,719 HSPs: Score = 380 (57.0 bits), Expect = 7.3e-31, Sum P(2) = 7.3e-31 Identities = 139/447 (31%), Positives = 207/447 (46%) Query: 462 RGHTPLHVAAVCGQASLIDLLVSKGAMVNATDYHGATPLHLACQKGYQΞVTLLLLHYKAS 521 +G+T LH+AA+ GQ ++ LV+ GA VNA G TPL++A Q+ + V LL A+ Sbjct: 77 KGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGAN 136 Query: 522 AEVQDNNGNTPLHLACTYGHEDCVKALVYYDVES-CRL 558 V +G TPL +A GHE+ V L+ Y + RL Sbjct: 137 QNVATEDGFTPLAVALQQGHENVVAHLINYGTKGKVRLPALHIAARNDDTRTAAVLLQND 196 Query: 559 DIGNEKGDTPLHIAARWGYQGVIETLLQNGASTEIQNRLKETPLKCALNSKILSVME 615 D+ ++ G TPLHIAA + V + LL GAS + TPL A S+ +V+ Sbjct: 197 PNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGASVNFTPQNGITPLHIA—SRRGNVIM 254 Query: 616 AYHLSFERRQKSSEAPVQSPQRSVDSISQESSTS-SFSSMSAGSR-QEETKKDYREVEKL 673 L +R + E + + ++ S + G+ Q +TK + Sbjct: 255 V-RLLLDRGAQI-ETKTKDELTPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHM- 311 Query: 674 LRAVADGD-LEMVRYLLEWTEEDLEDAEDTVSAADPEFCHPLCQCPKCAPAQKRLAKVPA 732 A GD L+ VR LL++ E ++D T+ P H C R+AKV Sbjct: 312 AAQGDHLDCVRLLLQYDAE-IDDI—TLDHLTP--LHVAAHC GHHRVAKVLL 358 Query: 733 S-GLGVNVTSQDGSSPLHVAALHGRADLIRLLLKHGANAGARNADQAVPLHLACQQGHFQ 791 G N + +G +PLH+A ++ LLLK GA+ A PLH+A GH Sbjct: 359 DKGAKPNSRALNGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLP 418 Query: 792 VVKCLLDSNAKPNKKDLSGNTPLIYACSGGHHELVALLLQHGASINASNNKGNTALHEAV 851 +VK LL A PN ++ TPL A GH E+ LLQ+ A +NA T LH A Sbjct: 419 IVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAA 478 Query: 852 IEKHVFVVELLLLHGASVQVLNKRQRTAVDCAEQNSKIMELLQVV 896 H +V+LLL + A+ + T + A + + +L ++ Sbjct: 479 RIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALL 523 Score = 378 (56.7 bits), Expect = 1.2e-30, Sum P(2) = 1.2e-30 Identities = 130/447 (29%), Positives = 195/447 (43%) Query: 465 TPLHVAAVCGQASLIDLLVSKGAMVNATDYHGATPLHLACQKGYQSVTLLLLHYKASAEV 524 TPLH AA G + ++L+ GA + A +G +P+H+A Q + LLL Y A + Sbjct: 274 TPLHCAARNGHVRISEILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDD 333 Query: 525 QDNNGNTPLHLACTYGHEDCVKALVYYDVE SCR 557 + TPLH+A GH K L+ + +C+ Sbjct: 334 ITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLLKT 393 Query: 558 LDIGNEKGDTPLHIAARWGYQGVIETLLQNGASTEIQNRLKETPLKCALNSKILSVM 614 +D E G TPLH+A+ G+ +++ LLQ GAS + N ETPL A + V Sb j ct: 394 GASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAGHTEVA 453 Query: 615 EAYHLSFERRQKSSEAPVQΞPQRSVDSISQESSTSSFΞSMSAGSRQEETKKDYREVEKLL 674 + Y L + + + Q+P I + +A T L Sbjct: 454 K-YLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLENNANPNLATTAGH TPLH 508 Query: 675 RAVADGDLEMVRYLLEWTEEDLEDAEDTVSAADPEFCHPLCQCPKCAPAQKRLAKVPASG 734 A +G +E V LLE ++ A T P H + K A+ L + Sbjct: 509 IAAREGHVETVLALLE KEASQACMTKKGFTP--LHVAAKYGKVRVAELLLER D 559 Query: 735 LGVNVTSQDGSSPLHVAALHGRADLIRLLLKHGANAGARNADQAVPLHLACQQGHFQVVK 794 N ++G +PLHVA H D+++LLL G + + + PLH+A +Q +V + Sbjct: 560 AHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQVEVAR 619 Query: 795 CLLDSNAKPNKKDLSGNTPLIYACSGGHHELVALLLQHGASINASNNKGNTALHEAVIEK 854 LL N + + G TPL A GH E+VALLL A+ N N G T LH E Sbjct: 620 SLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEG 679 Query: 855 HVFVVELLLLHGASVQVLNKRQRTAVDCAEQ--NSKIMELL 893 HV V ++L+ HG V + T + A N K+++ L Sbjct: 680 HVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFL 720 Score = 367 (55.1 bits), Expect = 1.8e-29, Sum P(2) = 1.8e-29 Identities = 131/489 (26%), Positives = 210/489 (42%) Query: 404 HIAS—GNQKEVERLLSQEDHDKDTVQKMCHPL-CFCDDCEKLVSGRLNDPΞVVTPFSRD 460 HIAS GN V LL + + + PL C + +S L D ++ Sbjct: 244 HIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRISEILLDHGAPIQ-AKT 302 Query: 461 DRGHTPLHVAAVCGQASLIDLLVSKGAMVNATDYHGATPLHLACQKGYQSVTLLLLHYKA 520 G +P+H+AA + LL+ A ++ TPLH+A G+ V +LL A Sbjct: 303 KNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGA 362 Query: 521 SAEVQDNNGNTPLHLACTYGHEDCVKALVYYDVESCRLDIGNEKGDTPLHIAARWGYQGV 580 + NG TPLH+AC H ++ L+ +D E G TPLH+A+ G+ + Sbjct: 363 KPNSRALNGFTPLHIACKKNHVRVMELLLK TGASIDAVTESGLTPLHVASFMGHLPI 419 Query: 581 IETLLQNGASTEIQNRLKETPLKCAL NSKILSVMEAYHLSFERRQKSSEAPVQSPQR 637 ++ LLQ GAS + N ETPL A ++++ + + K + P+ R Sbjct: 420 VKNLLQRGASPNVSNVKVETPLHMAARAGHTEVAKYLLQNKAKVNAKAKDDQTPLHCAAR 479 Query: 638 SVDSISQESSTSSFSSMSAGSRQEETKKDYREVEKLLRAVADGDLEMVRYLLEWTE 693 ++ + E++ + + +AG VE +L + + +T Sbjct: 480 IGHTNMVKLLLENNANPNLATTAGHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTP 539 Query: 694 EDLEDAEDTVSAAD PEFCHPLCQ CP-KCAPAQKRLAKVPA SGLGVNVTS 741 + V A+ HP P A L V G + + Sbjct: 540 LHVAAKYGKVRVAELLLERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPA 599 Query: 742 QDGSSPLHVAALHGRADLIRLLLKHGANAGARNADQAVPLHLACQQGHFQVVKCLLDSNA 801 +G +PLH+AA + ++ R LL++G +A A + PLHLA Q+GH ++V LL A Sbjct: 600 WNGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQA 659 Query: 802 KPNKKDLSGNTPLIYACSGGHHELVALLLQHGASINASNNKGNTALHEAVIEKHVFVVEL 861 N + SG TPL GH + +L++HG ++A+ G T LH A ++ +V+ Sbjct: 660 NGNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKF 719 Query: 862 LLLHGASVQVLNK 874 LL H A V K Sbjct: 720 LLQHQADVNAKTK 732 Score = 345 (51.8 bits), Expect = 4.2e-27, Sum P(2) = 4.2e-27 Identities = 146/506 (28%), Positives = 233/506 (46%) Query: 404 HIAS--GNQKEVERLLSQEDHDKDTVQK MCHPLCFCDDCEKLVSGRLNDPSVVTPFS 458 H+AS G+ K V LL +E + T +K H +++V +N + V + Sbjct: 50 HLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQ-DEVVRELVNYGANVN—A 106 Query: 459 RDDRGHTPLHVAAVCGQASLIDLLVSKGAMVNATDYHGATPLHLACQKGYQSVTLLLLHY 518 + +G TPL++AA ++ L+ GA N G TPL +A Q+G+++V L++Y Sbjct: 107 QSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVALQQGHENVVAHLINY 166 Query: 519 KASAEVQDNNGNTP-LHLACTYGHEDCVKALVYYDVESCRLDIGNEKGDTPLHIAARWGY 577 +V+ P LH+A ++D A V + D+ ++ G TPLHIAA + Sbjct: 167 GTKGKVR LPALHIAAR—NDDTRTAAVLLQNDP-NPDVLSKTGFTPLHIAAHYEN 218 Query: 578 QGVIETLLQNGASTEIQNRLKETPLKCAL NSKILSVMEAYHLSFERRQKSSEAPVQS 634 V + LL GAS + TPL A N ++ ++ E + K P+ Sbjct: 219 LNVAQLLLNRGASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHC 278 Query: 635 PQRSVDSISQESSTSSFSSMSAGSRQEETKKDYREVEKLLRAVADGD-LEMVRYLLEWTE 693 R+ E + + A +TK + A GD L+ VR LL++ Sbjct: 279 AARNGHVRISEILLDHGAPIQA KTKNGLΞPIHM AAQGDHLDCVRLLLQYDA 329 Query: 694 EDLEDAE-DTVSAAD-PEFC--HPLCQC PK CAPAQKRLAK 729 E ++D D ++ C H + + P C R+ + Sbjct: 330 E-IDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVME 388 Query: 730 VPA-SGLGVNVTSQDGSSPLHVAALHGRADLIRLLLKHGANAGARNADQAVPLHLACQQG 788 + +G ++ ++ G +PLHVA+ G +++ LL+ GA+ N PLH+A + G Sb ct: 389 LLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRGASPNVSNVKVETPLHMAARAG 448 Query: 789 HFQVVKCLLDSNAKPNKKDLSGNTPLIYACSGGHHELVALLLQHGASINASNNKGNTALH 848 H +V K LL + AK N K TPL A GH +V LLL++ A+ N + G+T LH Sbjct: 449 HTEVAKYLLQNKAKVNAKAKDDQTPLHCAARIGHTNMVKLLLENNANPNLATTAGHTPLH 508 Query: 849 EAVIEKHVFVVELLLLHGASVQVLNKRQRTAVDCAEQNSKIM—ELL 893 A E HV V LL AS + K+ T + A + K+ ELL Sbjct: 509 IAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELL 555 Score = 243 (36.5 bits), Expect = 1.6e-14, Sum P(2) = 1.6e-14 Identities = 64/199 (32%), Positives = 97/199 (48%) Query: 404 HIAS—GNQKEVERLLSQEDHDKDTVQKMCHPLCFCDDCEKLVSGRLNDPSVVTPFSRDD 461 H+A+ G + E LL ++ H + PL L +L P +P S Sbjct: 541 HVAAKYGKVRVAELLLERDAHPNAAGKNGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAW 600 Query: 462 RGHTPLHVAAVCGQASLIDLLVSKGAMVNATDYHGATPLHLACQKGYQSVTLLLLHYKAS 521 G+TPLH+AA Q + L+ G NA G TPLHLA Q+G+ + LLL +A+ Sbjct: 601 NGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQAN 660 Query: 522 AEVQDNNGNTPLHLACTYGHEDCVKALVYYDVESCRLDIGNEKGDTPLHIAARWGYQGVI 581 + + +G TPLHL GH L+ + V +D G TPLH+A+ +G ++ Sbjct: 661 GNLGNKSGLTPLHLVAQEGHVPVADVLIKHGV MVDATTRMGYTPLHVASHYGNIKLV 717 Query: 582 ETLLQNGASTEIQNRLKETPL 602 + LLQ+ A + +L +PL Sbjct: 718 KFLLQHQADVNAKTKLGYSPL 738 Score = 242 (36.3 bits), Expect = 5.0e-29, Sum P(2) = 5.0e-29 Identities = 63/176 (35%), Positives = 92/176 (52%) Query: 734 GLGVNVTSQDGSSPLHVAALHGRADLIRLLLKHGANAGARNADQAVPLHLACQQGHFQVV 793 G VN T Q+G +PLH+A+ G ++RLLL GA + D+ PLH A + GH ++ Sbjct: 229 GASVNFTPQNGITPLHIASRRGNVIMVRLLLDRGAQIETKTKDELTPLHCAARNGHVRIS 288 Query: 794 KCLLDSNAKPNKKDLSGNTPLIYACSGGHHELVALLLQHGASINASNNKGNTALHEAVIE 853 + LLD A K +G +P+ A G H + V LLLQ+ A 1+ T LH A Sbjct: 289 EILLDHGAPIQAKTKNGLSPIHMAAQGDHLDCVRLLLQYDAEIDDITLDHLTPLHVAAHC 348 Query: 854 KHVFVVELLLLHGA—SVQVLNKRQRTAVDCAEQNSKIMELLQVVPSCVASLDDVAET 909 H V ++LL GA + + LN + C + + ++MELL AS+D V E+ Sbjct: 349 GHHRVAKVLLDKGAKPNSRALNGFTPLHIACKKNHVRVMELLLKTG ASIDAVTES 403 Score = 242 (36.3 bits), Expect = 3.3e-14, Sum P(2) = 3.3e-14 Identities = 80/284 (28%), Positives = 129/284 (45%) Query: 404 HIAS—GNQKEVERLLSQEDHDKDTVQKMCHPLCFCDDCEKLVΞGRLNDPSVVTPFSRDD 461 HIA+ G+ + V LL +E +K PL K+ L P + Sbjct: 508 HIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAHPNAAGK 567 Query: 462 RGHTPLHVAAVCGQASLIDLLVSKGAMVNATDYHGATPLHLACQKGYQSVTLLLLHYKAS 521 G TPLHVA ++ LL+ +G ++ ++G TPLH+A ++ V LL Y S Sbjct: 568 NGLTPLHVAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQVEVARSLLQYGGS 627 Query: 522 AEVQDNNGNTPLHLACTYGHEDCVKALVYYDVESCRLDIGNEKGDTPLHIAARWGYQGVI 581 A + G TPLHLA GH + V L+ ++GN+ G TPLH+ A+ G+ V Sbjct: 628 ANAESVQGVTPLHLAAQEGHAEMVALLLSKQANG NLGNKSGLTPLHLVAQEGHVPVA 684 Query: 582 ETLLQNGASTEIQNRLKETPLKCAL NSKILΞVMEAYHLSFERRQKSSEAPV-QSPQR 637 + L+++G + R+ TPL A N K++ + + + K +P+ Q+ Q+ Sbjct: 685 DVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQ 744 Query: 638 S-VDSISQ—ESSTSSFSSMSAGSRQEETKK—DYREVEKLLRAVAD 679 D ++ ++ Ξ S G+ K Y V +L+ V D Sbjct: 745 GHTDIVTLLLKNGASPNEVSSDGTTPLAIAKRLGYISVTDVLKVVTD 791 Score = 235 (35.3 bits), Expect = 7.9e-34, Sum P(2) = 7.9e-34 Identities = 58/165 (35%), Positives = 83/165 (50%) Query: 734 GLGVNVTSQDGSSPLHVAALHGRADLIRLLLKHGANAGARNADQAVPLHLACQQGHFQVV 793 G N S G +PLH+AA G A+++ LLL AN N PLHL Q+GH V Sbjct: 625 GGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQANGNLGNKSGLTPLHLVAQEGHVPVA 684 Query: 794 KCLLDSNAKPNKKDLSGNTPLIYACSGGHHELVALLLQHGASINASNNKGNTALHEAVIE 853 L+ + G TPL A G+ +LV LLQH A +NA G + LH+A + Sbjct: 685 DVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLLQHQADVNAKTKLGYSPLHQAAQQ 744 Query: 854 KHVFVVELLLLHGASVQVLNKRQRTAVDCAEQNS—KIMELLQVV 896 H +V LLL +GAS ++ T + A++ + ++L+VV Sbjct: 745 GHTDIVTLLLKNGASPNEVΞSDGTTPLAIAKRLGYISVTDVLKVV 789 Score = 233 (35.0 bits), Expect = 7.9e-34, Sum P(2) = 7.9e-34 Identities = 67/202 (33%), Positives = 100/202 (49%) Query: 404 HIAS-GNQKEVERLLSQEDHDKDTVQKMCH—PLCFCDDC-EKLVSGRLNDPSVVTPFSR 459 H+A+ G+ + RLL Q D + D + + H PL C V+ L D P SR Sbjct: 310 HMAAQGDHLDCVRLLLQYDAEIDDIT-LDHLTPLHVAAHCGHHRVAKVLLDKGA-KPNSR 367 Query: 460 DDRGHTPLHVAAVCGQASLIDLLVSKGAMVNATDYHGATPLHLACQKGYQSVTLLLLHYK 519 G TPLH+A +++LL+ GA ++A G TPLH+A G+ + LL Sbjct: 368 ALNGFTPLHIACKKNHVRVMELLLKTGASIDAVTESGLTPLHVASFMGHLPIVKNLLQRG 427 Query: 520 ASAEVQDNNGNTPLHLACTYGHEDCVKALVYYDVESCRLDIGNEKGDTPLHIAARWGYQG 579 AS V + TPLH+A GH + K L+ +++ + TPLH AAR G+ Sbjct: 428 ASPNVSNVKVETPLHMAARAGHTEVAKYLLQ NKAKVNAKAKDDQTPLHCAARIGHTN 484 Query: 580 VIETLLQNGASTEIQNRLKETPLKCA 605 +++ LL+N A+ + TPL A Sbjct: 485 MVKLLLENNANPNLATTAGHTPLHIA 510 Score = 226 (33.9 bits), Expect = 7.0e-33, Sum P(2) = 7.0e-33 Identities = 53/153 (34%), Positives = 83/153 (54%) Query: 743 DGSSPLHVAALHGRADLIRLLLKHGANAGARNADQAVPLHLACQQGHFQVVKCLLDSNAK 802 +G +PLH+AA + ++ R LL++G +A A + PLHLA Q+GH ++V LL A Sbjct: 601 NGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQGVTPLHLAAQEGHAEMVALLLSKQAN 660 Query: 803 PNKKDLSGNTPLIYACSGGHHELVALLLQHGASINASNNKGNTALHEAVIEKHVFVVELL 862 N + SG TPL GH + +L++HG ++A+ G T LH A ++ +V+ L Sbjct: 661 GNLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFL 720 Query: 863 LLHGASVQVLNKRQRTAVDCAEQ--NSKIMELL 893 L H A V K + + A Q ++ I+ LL Sbjct: 721 LQHQADVNAKTKLGYSPLHQAAQQGHTDIVTLL 753 Score = 198 (29.7 bits), Expect = 2.5e-ll, Sum P(2) = 2.5e-ll Identities = 51/157 (32%), Positives = 82/157 (52%) Query: 737 VNVTSQDGSSPLHVAALHGRADLIRLLLKHGANAGARNADQAVPLHLACQQGHFQVVKCL 796 + T++ G++ LH+AAL G+ +++R L+ +GAN A++ PL++A Q+ H +VVK L Sbjct: 71 LETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFL 130 Query: 797 LDSNAKPNKKDLSGNTPLIYACSGGHHELVALLLQHGASINASNNKGNTALHEAVIEKHV 856 L++ A N G TPL A GH +VA L+ +G ALH A Sbjct: 131 LENGANQNVATEDGFTPLAVALQQGHENVVAHLINYGTK GKVRLPALHIAARNDDT 186 Query: 857 FVVELLLLHGASVQVLNKRQRTAVDCAE—QNΞKIMELL 893 +LL + + VL+K T + A +N + +LL Sbjct: 187 RTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLL 225 Score = 186 (27.9 bits), Expect = 6.6e-29, Sum P(2) = 6.6e-29 Identities = 55/143 (38%), Positives = 68/143 (47%) Query: 463 GHTPLHVAAVCGQASLIDLLVSKGAMVNATDYHGATPLHLACQKGYQSVTLLLLHYKASA 522 GHTPLH+AA G + L+ K A G TPLH+A + G V LLL A Sb j ct: 503 GHTPLHIAAREGHVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAHP 562 Query: 523 EVQDNNGNTPLHLACTYGHEDCVKALVYYDVESCRLDIGNEKGDTPLHIAARWGYQGVIE 582 NG TPLH+A + + D VK L+ S N G TPLHIAA+ V Sbjct: 563 NAAGKNGLTPLHVAVHHNNLDIVKLLLPRG-GSPHSPAWN—GYTPLHIAAKQNQVEVAR 619 Query: 583 TLLQNGASTEIQNRLKETPLKCA 605 +LLQ G S ++ TPL A Sbjct: 620 SLLQYGGSANAESVQGVTPLHLA 642 Score = 182 (27.3 bits), Expect = 2.9e-28, Sum P(2) = 2.9e-28 Identities = 54/185 (29%), Positives = 89/185 (48%) Query: 738 NVTSQDGSSPLHVAALHGRADLIRLLLKHGANAGARNADQAVPLHLACQQGHFQVVKCLL 797 N+ ++ G +PLH+ A G + +L+KHG A PLH+A G+ ++VK LL Sbjct: 662 NLGNKSGLTPLHLVAQEGHVPVADVLIKHGVMVDATTRMGYTPLHVASHYGNIKLVKFLL 721 Query: 798 DΞNAKPNKKDLSGNTPLIYACSGGHHELVALLLQHGASINASNNKGNTALHEAVIEKHVF 857 A N K G +PL A GH ++V LLL++GAS N ++ G T L A ++ Sbjct: 722 QHQADVNAKTKLGYSPLH Q AAQQGHTDIVTLLLKNGASPNEVSSDGTTPLAIAKRLGYIS 781 Query: 858 VVELLLLHGASVQVLNKRQRTAVDCAEQNSKIMELLQVVPSCVASLDDVAETDRKEYVTV 917 V ++L + V ++ V + S P V + DV+E + +E ++ Sbjct: 782 VTDVLKV VTDETSFVLVSDKHRMS FPETVDEILDVSEDEGEELISF 827 Query: 918 KIRKK 922 K ++ Sbjct: 828 KAERR 832 Score = 180 (27.0 bits), Expect = 5.0e-29, Sum P(2) = 5.0e-29 Identities = 41/121 (33%), Positives = 67/121 (55%) Query: 486 GAMVNATDYHGATPLHLACQKGYQSVTLLLLHYKASAEVQDNNGNTPLHLACTYGHEDCV 545 G +N + +G LHLA ++G+ + + LLH + E GNT LH+A G ++ V Sbjct: 35 GVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVV 94 Query: 546 KALVYYDVESCRLDIGNEKGDTPLHIAARWGYQGVIETLLQNGASTEIQNRLKETPLKCA 605 + LV Y ++ ++KG TPL++AA+ + V++ LL+NGA+ + TPL A Sbjct: 95 RELVNY GANVNAQSQKGFTPLYMAAQENHLEVVKFLLENGANQNVATEDGFTPLAVA 151 Query: 606 L 606 L Sbjct: 152 L 152 Score = 166 (24.9 bits), Expect = 3.4e-06, Sum P(2) = 3.4e-06 Identities = 89/318 (27%), Positives = 140/318 (44%) Query: 448 LNDPSVVTPFSRDDRGHTPLHVAAVCGQASLIDLLVSKGAMVNATDYHGATPLHLACQKG 507 L + + V ++DD+ TPLH AA G +++ LL+ A N G TPLH+A ++G Sbjct: 457 LQNKAKVNAKAKDDQ—TPLHCAARIGHTNMVKLLLENNANPNLATTAGHTPLHIAAREG 514 Query: 508 YQSVTLLLLHYKASAEVQDNNGNTPLHLACTYGHEDCVKALVYYD 552 + L LL +AS G TPLH+A YG + L+ D Sbjct: 515 HVETVLALLEKEASQACMTKKGFTPLHVAAKYGKVRVAELLLERDAHPNAAGKNGLTPLH 574 Query: 553 —VESCRLDI GNE KGDTPLHIAARWGYQGVIETLLQNGASTEIQNRL 597 V LDI G+ G TPLHIAA+ V +LLQ G S ++ Sbjct: 575 VAVHHNNLDIVKLLLPRGGSPHSPAWNGYTPLHIAAKQNQVEVARSLLQYGGSANAESVQ 634 Query: 598 KETPLKCALNSKILSVMEAYHLSFERRQKSSEAPVQΞPQRSVDSISQESSTSSFSSM-SA 656 TPL A M A LS +Q + +S + ++QE + Sbjct: 635 GVTPLHLAAQEGHAE-MVALLLS KQANGNLGNKSGLTPLHLVAQEGHVPVADVLIKH 690 Query: 657 GSRQEETKKDYREVEKLLRAVADGDLEMVRYLLEWTEEDLEDAEDTVSAADPEFCHPLCQ 716 G + T + L A G++++V++LL+ + D+ +A+ + + PL Q Sbjct: 691 GVMVDATTR— GYTPLHVASHYGNIKLVKFLLQH-QADV-NAKTKLGYΞ PLHQ 740 Query: 717 CPKCAPAQKRLAKVPASGLGVNVTSQDGSSPLHVA 751 + + + +G N S DG++PL +A Sbjct: 741 AAQQGHTDI-VTLLLKNGASPNEVSSDGTTPLAIA 774 Score = 162 (24.3 bits), Expect = 1.8e-07, Sum P(2) = 1.8e-07 Identities = 48/149 (32%), Positives = 71/149 (47%) Query: 737 VNVTSQDGSSPLHVAALHGRADLIRLLLKHGANAGARNADQAVPLHLACQQGHFQVVKCL 796 V D ++ AA G D L++G + N + LHLA ++GH ++V L Sbjct: 5 VGFREADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVEL 64 Query: 797 LDSNAKPNKKDLSGNTPLIYACSGGHHELVALLLQHGASINASNNKGNTALHEAVIEKHV 856 L GNT L A G E+V L+ +GA++NA + KG T L+ A E H+ Sbjct: 65 LHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHL 124 Query: 857 FVVELLLLHGASVQVLNKRQRTAVDCAEQ 885 VV+ LL +GA+ V + T + A Q Sbjct: 125 EVVKFLLENGANQNVATEDGFTPLAVALQ 153 Score = 158 (23.7 bits), Expect = 5.7e-26, Sum P(2) = 5.7e-26 Identities = 38/135 (28%), Positives = 65/135 (48%) Query: 460 DDRGHTPLHVAAVCGQASLIDLLVSKGAMVNATDYHGATPLHLACQKGYQSVTLLLLHYK 519 + G LH+A+ G ++ L+ K ++ T G T LH+A G V L++Y Sbjct: 42 NQNGLNGLHLASKEGHVKMVVELLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYG 101 Query: 520 ASAEVQDNNGNTPLHLACTYGHEDCVKALVYYDVESCRLDIGNEKGDTPLHIAARWGYQG 579 A+ Q G TPL++A H + VK L+ ++ E G TPL +A + G++ Sbjct: 102 ANVNAQSQKGFTPLYMAAQENHLEVVKFLLE NGANQNVATEDGFTPLAVALQQGHEN 158 Query: 580 VIETLLQNGASTEIQ 594 V+ L+ G +++ Sbjct: 159 VVAHLINYGTKGKVR 173 Score = 115 (17.3 bits), Expect = 1.8e-21, Sum P(2) = 1.8e-21 Identities = 37/119 (31%), Positives = 58/119 (48%) Query: 497 ATPLHLACQKGYQSVTLLLLHYKASAEVQ—DNNGNTPLHLACTYGHEDCVKALVYYDVE 554 AT A + G ++ L H + ++ + NG LHLA GH V L++ ++ Sbjct: 13 ATΞFLRAARΞG—NLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVELLHKEII 70 Query: 555 SCRLDIGNEKGDTPLHIAARWGYQGVIETLLQNGASTEIQNRLKETPLKCALNΞKILSVM 614 L+ +KG+T LHIAA G V+ L+ GA+ Q++ TPL A L V+ Sbjct: 71 LETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENHLEVV 127 Query: 615 E 615 + Sbjct: 128 K 128 Score = 106 (15.9 bits), Expect = 1.8e-01, Sum P(2) = 1.6e-01 Identities = 34/121 (28%), Positives = 54/121 (44%) Query: 769 NAGARNADQAVPLHLACQQGHFQVVKCLLDSNAKPNKKDLSGNTPLIYACSGGHHELVAL 828 + G R AD A A + G+ L + N + +G L A GH ++V Sbjct: 4 SVGFREADAATSFLRAARSGNLDKALDHLRNGVDINTCNQNGLNGLHLASKEGHVKMVVE 63 Query: 829 LLQHGASINASNNKGNTALHEAVIEKHVFVVELLLLHGASVQVLNKRQRTAVDCAEQNSK 888 LL + + KGNTALH A + VV L+ +GA+V +++ T + A Q + Sbjct: 64 LLHKEIILETTTKKGNTALHIAALAGQDEVVRELVNYGANVNAQSQKGFTPLYMAAQENH 123 Query: 889 I 889 + Sbjct: 124 L 124 Score = 40 (6.0 bits), Expect = 1.6e-14, Sum P(2) 1.6e-14 Identities = 11/56 (19%), Positives = 23/56 (41%) Query: 622 ERRQKSSEAPVQSPQRSVDSISQESSTSSFSSMSAGSRQEETKKDYREVEKLLRAV 677 +RRQ+ E VQ + + + Q + + Q ++ +K++R V Sbjct: 1614 DRRQQGQEEQVQEAKNTFTQVVQGNEFQNIPGEQVTEEQFTDEQGNIVTKKIIRKV 1669 Score = 38 (5.7 bits), Expect = 2.6e-14, Sum P(2) = 2.6e-14 Identities = 6/12 (50%), Positives = 10/12 (83%) Query: 806 KDLSGNTPLIYA 817 +D++G T L+YA Sbjct: 1186 EDITGTTKLVYA 1197 Pedant information for DKFZphtes3_1817, frame 2 Report for DKFZphtes3_1817.2 [LENGTH] 1050 [MW] 117013.72 [pl] 6.47 [HOMOL] TREMBL : DMANKY_1 product: "ankyrin"; Drosophila melanogaster ankyrin mRNA, complete eds . 2e-45 [FUNCAT] 08.19 cellular import [S. cerevisiae, YOR034c] 5e-13 [FUNCAT] 10.05.99 other pheromone response activities [S. cerevisiae, YDR264c] 3e-12 [FUNCAT] 03.07 pheromone response, mating-type determination, sex-specific proteins [ S. cerevisiae, YDR264c] 3e-12 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YIL112w] 2e-ll [FUNCAT] 06.13.01 cytoplasmic degradation [S. cerevisiae, YGR232w] 8e-10 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YIR033w] 2e-08 [FUNCAT] 04.05.01.07 chromatin modification [S. cerevisiae, YIR033w] 2e-08 [FUNCAT] 01.04.04 regulation of phosphate utilization [S. cerevisiae, YGR233c] 3e-08 [FUNCAT] 08.13 vacuolar transport [S. cerevisiae, YML097c] 5e-05 [FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YML097c] 5e-05 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YML097C] 5e-05 [FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YML097c] 5e-05 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YERlllc] 3e-04 [ FUNCA ] 04.05.01.04 transcriptional control [S. cerevisiae, YERlllc] 3e-04 [BLOCKS] BL00901A Cysteine synthase/cystathionme beta-synthase P-phosphate att [SCOP] dlawcb_ 1.91.3.1.2 GA binding protein (GABP) alpha GA bindini 4e-12 [EC] 3.1.3.53 Myosm-light-chain-phosphatase le-12 [PIRKW] phosphotransferase le-19 [PIRKW] nucleus le-13 [PIRKW] potassium channel 5e-15 [PIRKW] early protein 2e-13 [PIRKW] tumor suppressor le-09 [PIRKW] duplication le-14 [PIRKW] tandem repeat le-19 [PIRKW] heterodimer le-14 [PIRKW] potassium transport 5e-15 [PIRKW] cell cycle control le-10 [PIRKW] serine/threon e-specific protein kmase le-19 [PIRKW] transmembrane protein 5e-15 [PIRKW] transport protein 5e-15 [PIRKW] DNA binding 2e-ll [PIRKW] oncogene le-08 [PIRKW] ATP le-19 [PIRKW] protein kinase inhibitor le-09 [PIRKW] voltage-gated ion channel 5e-15 [PIRKW] phosphoprotein 4e-38 [PIRKW] apoptosis le-19 [PIRKW] liver 4e-09 [PIRKW] mtegπn binding 3e-16 [PIRKW] differentiation 2e-12 [PIRKW] transforming protein le-08 [PIRKW] alternative splicing le-40 [PIRKW] coiled coil le-14 [PIRKW] peripheral membrane protein 2e-38 [PIRKW] transcription factor 4e-16 [PIRKW] transcription regulation 2e-16 [PIRKW] nucleotide binding 5e-15 [PIRKW] phosphoric monoester hydrolase le-12 [PIRKW] cytoskeleton 8e-39 [PIRKW] calmodulin binding le-19 [PIRKW] smooth muscle le-12 [SUPFAM] ankyrin le-40 [SUPFAM] death-associated protein kinase le-19 [SUPFAM] ankyrin repeat homology le-40 [SUPFAM] protein kinase homology le-19 [SUPFAM] vaccinia virus 27.4K Hindlll-C protein homology 3e-07 [SUPFAM] mt-3 transforming protein le-08 [SUPFAM] unassigned ankyrin repeat proteins 2e-38 [SUPFAM] notch protein 2e-12 [SUPFAM] fowlpox virus BamHI-ORF7 protein 2e-13 [SUPFAM] rel homology 2e-ll [SUPFAM] EGF homology 2e-12 [PROSITE] ATP_GTP_A 1 [PFAM] Ank repeat [KW] Irregular [KW] 3D [KW] LOW_COMPLEXITY 3.05 % SEQ MALYDEDLLKNPFYLALQKCRPDLCSKVAQIHGIVLVPCKGSLSSSIQSTCQFESYILIP SEG lawcB SEQ VEEHFQTLNGKDVFIQGNRIKLGAGFACLLSVPILFEETFYNEKEESFSILCIAHPLEKR SEG lawcB SEQ ESSEEPLAPSDPFSLKTIEDVREFLGRHSERFDRNIASFHRTFRECERKSLRHHIDSANA SEG lawcB SEQ LYTKCLQQLLRDSHLKMLAKQEAQMNLMKQAVEIYVHHEIYNLIFKYVGTMEAΞEDAAFN SEG lawcB SEQ KITRSLQDLQQKDIGVKPEFSFNIPRAKRELAQLNKCTSPQQKLVCLRKVVQLITQSPSQ SEG lawcB SEQ RVNLETMCADDLLSVLLYLLVKTEIPNWMANLSYIKNFRFSSLAKDELGYCLTSFEAAIE SEG xxxxxxxxxx lawcB SEQ YIRQGSLSAKPPESEGFGDRLFLKQRMSLLSQMTSSPTDCLFKHIASGNQKEVERLLSQE SEG lawcB SEQ DHDKDTVQKMCHPLCFCDDCEKLVSGRLNDPSVVTPFSRDDRGHTPLHVAAVCGQASLID SEG lawcB SEQ LLVSKGAMVNATDYHGATPLHLACQKGYQSVTLLLLHYKASAEVQDNNGNTPLHLACTYG SEG lawcB SEQ HEDCVKALVYYDVESCRLDIGNEKGDTPLHIAARWGYQGVIETLLQNGASTEIQNRLKET SEG lawcB SEQ PLKCALNSKILSVMEAYHLSFERRQKSSEAPVQSPQRSVDSISQESSTSSFSSMSAGSRQ SEG xxxxxxxxxxxxxxxxxxxxxx . lawcB SEQ EETKKDYREVEKLLRAVADGDLEMVRYLLEWTEEDLEDAEDTVSAADPEFCHPLCQCPKC SEG lawcB SEQ APAQKRLAKVPASGLGVNVTSQDGSSPLHVAALHGRADLIRLLLKHGANAGARNADQAVP SEG lawcB CHHHHHHHHHHHCCHHHHHHHHHHCCCC-CCTTTTCCH SEQ LHLACQQGHFQVVKCLLDSNAKPNKKDLSGNTPLIYACSGGHHELVALLLQHGASINASN SEG lawcB HHHHHHHCCHHHHHHHHHCCCTTTTCTTTTCCHHHHHHHHTTHHHHHHHHHCCCTTTTEE SEQ NKGNTALHEAVIEKHVFVVELLLLHGASVQVLNKRQRTAVDCAEQNSKIMELLQVVPΞCV SEG lawcB TTTEEHHHHHHHHCCHHHHHHHHHHCCTTTTCBTTTBCHHHHHHHHCCHHHHHC SEQ ASLDDVAETDRKEYVTVKIRKKWNSKLYDLPDEPFTRQFYFVHSAGQFKGKTSREIMARD SEG lawcB SEQ RSVPNLTEGSLHEPGRQΞVTLRQNNLPAQSGSHAAEKGNSDWPERPGLTQTGPGHRRMLR SEG lawcB SEQ RHTVEDAVVSQGPEAAGPLSTPQEVSASRS SEG lawcB Prosite for DKFZphtes3_1817.2 PΞ00017 945->953 ATP GTP A PDOC00017 Pfam for DKFZphtes3_1817.2 HMM_NAME Ank repeat HMM *GyTPLHIAARyNNvEMVrlLLQHGADIN* G+TPLH+AA ++ ++++LL+++GA +N Query 463 GHTPLHVAAVCGQASLIDLLVSKGAMVN 490 32.12 (bits) f: 496 t: 523 Target: dkfzphtes3_1817.2 similarity to ankyrins Alignment to HMM consensus : Query *GyTPLHIAARyNNvEMVrlLLQHGADIN* G TPLH+A++ + ++ LLL + A+ dkfzphtes3 496 GATPLHLACQKGYQSVTLLLLHYKASAE 523 Query f: 529 t: 556 Target: dkfzphtes3_1817.2 similarity to ankyrins Alignment to HMM consensus: HMM *GyTPLHIAARyNNvEMVrlLLQHGADIN* G+TPLH+A+ Y+++++V+ L+ + Query 529 GNTPLHLACTYGHEDCVKALVYYDVESC 556 42.65 (bits) f: 565 t: 592 Target: dkfzphtes3_1817.2 similarity to ankyrins Alignment to HMM consensus: Query *GyTPLHIAARyNNvEMVrlLLQHGADIN* G+TPLHIAAR + +++ LLQ+GA+ dkfzphtes3 565 GDTPLHIAARWGYQGVIETLLQNGAΞTE 592 Query f: 744 t: 771 Target: dkfzphtes3_1817.2 similarity to ankyrins Alignment to HMM consensus: HMM *GyTPLHIAARyNNvEMVrlLLQHGADIN* G +PLH+AA +++ +++RLLL+HGA+ Query 744 GSSPLHVAALHGRADLIRLLLKHGANAG 771 36.38 (bits) f: 777 t: 804 Target: dkfzphtes3_1817.2 similarity to ankyrins Alignment to HMM consensus: Query *GyTPLHIAARyNNvEMVrlLLQHGADIN* PLH+A+++++ ++V+ LL+ +A +N dkfzphtes3 777 QAVPLHLACQQGHFQVVKCLLDSNAKPN 804 Query f: 810 t: 837 Target: dkfzphtes3_1817.2 similarity to ankyrins Alignment to HMM consensus : HMM *GyTPLHIAARyNNvEMVrlLLQHGADIN* G+TPL++A+ ++ E+V LLLQHGA+IN Query 810 GNTPLIYACSGGHHELVALLLQHGASIN 837 44.62 (bits) f: 843 t: 870 Target: dkfzphtes3_1817.2 similarity to ankyrins Alignment to HMM consensus: Query *GyTPLHIAARyNNvEMVrlLLQHGADIN* G+T+LH A+++ +V +V+LLL HGA++ dkfzphtes3 843 GNTALHEAVIEKHVFVVELLLLHGASVQ 870 DKFZphtes3_19fl9 group: testes derived DKFZphtes3_19f19 encodes a novel 254 amino acid protein with weak similarity to S. cerevisiae protein YFL046w. The protein contains a RGD cell attachment site. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . similarity to YFL046w localisation: 3 STS match perfect but HS1292427 matches to chromosome 4 Sequenced by MediGenomix Locus: /map="405.0/.3 cR from top of Chrll linkage group" Insert length: 1395 bp Poly A stretch at pos. 1367, no polyadenylation signal found 1 GGGACCACGG TGGCGCCTGC GCTGGGAGGT GAGCTTGTGA CAGAGCGAAA 51 ACTACAATTC CCAGCATTCC TGTGGTGCCA GAACTACCTT GCCCGAAAGC 101 CTGTGCGAGA TTTACCCCGT CTTCCGCCTC CCTCCCACCG GAAAACTCTG 151 AGGACATGAA TAGTCGCCAG GCTTGGCGGC TCTTTCTCTC CCAAGGCAGA 201 GGAGATCGTT GGGTTTCAAG GCCCCGCGGG CATTTCTCGC CGGCCCTGCG 251 GAGAGAGTTC TTCACTACCA CAACCAAGGA GGGATATGAT AGGCGGCCAG 301 TGGATATAAC TCCTTTAGAA CAAAGGAAAT TAACTTTTGA TACCCATGCA 351 TTGGTTCAGG ACTTGGAAAC TCATGGATTT GACAAAACAC AAGCAGAAAC 401 AATTGTATCA GCGTTAACTG CTTTATCAAA TGTCAGCCTG GATACTATCT 451 ATAAAGAGAT GGTCACTCAA GCTCAACAGG AAATAACAGT ACAACAGCTA 501 ATGGCTCATT TGGATGCTAT CAGGAAAGAC ATGGTCATCC TAGAGAAAAG 551 TGAATTTGCA AATCTGAGAG CAGAGAATGA GAAAATGAAA ATTGAATTAG 601 ACCAAGTTAA GCAACAACTA ATGCATGAAA CCAGTCGAAT CAGAGCAGAT 651 AATAAACTGG ATATCAACTT AGAAAGGAGC AGAGTAACAG ATATGTTTAC 701 AGATCAAGAA AAGCAACTTA TGGAAACAAC TACAGAATTT ACAAAAAAGG 751 ATACTCAAAC CAAAAGTATT ATTTCAGAGA CCAGTAATAA AATTGACGCT 801 GAAATTGCTT CCTTAAAAAC ACTGATGGAA TCTAACAAAC TTGAGACAAT 851 TCGTTATCTT GCAGCTTCGG TGTTTACTTG CCTGGCAATA GCATTGGGAT 901 TTTATAGATT CTGGAAGTAG TATTAATGCT CATCCTGCTG TGGCTGTTGG 951 CTTCTTAGAA CACCAAACCG GGAGAGATTT ACTTTGAACA TTGTCAGTTG 1001 CAGCAAAAAT TTACTACACA AGATTATTCG AAGTGTATAC GGACTAAAAG 1051 AGGAAGTGTT TTAGAATGAG AAGAGATACT GTGTCTTTAT TGTGTGTGTG 1101 TGAGTGCAGG TGTGTGTCTT TATTATATTG AAAAGCTGTC ACTCAGACCT 1151 GGTTTGAGAT AGAAGAGCAT TTTGTCCTTT TGATAGTTAA TAGAAATTGA 1201 ACCAGAGTTT TCTTATGTTT GCTTGAACAG TTGTGTAAAT CATACAGGAT 1251 TTTGTGGGTA TTGGTTGAAT ATTTGTAAAC CATTCCCTAG CCTACATATT 1301 TATTACTGAA TTAACTTTCC TGATAACCAT TGCATAATTA CATTTTTCTA 1351 TAAAATGAAA GATTATTACA ACAAAAAAAA AAAAAAAAAA AAAAA BLAST Results Entry HS419346 from database EMBL: human STS WI-13569. Score = 2154, P = 8.6e-91, identities = 446/459 Entry HS1292427 from database EMBL: human STS SHGC-50338. Score = 1737, P = 7.2e-72, identities = 359/369 Entry HS253344 from database EMBL: human STS WI-13893. Score = 1578, P = 1.0e-64, identities = 358/397 Medline entries No Medline entry Peptide information for frame 3 ORF from 156 bp to 917 bp; peptide length: 254 Category: similarity to unknown protein Classification: no clue Prosite motifs: RGD (15-18) 1 MNSRQAWRLF LSQGRGDRWV SRPRGHFSPA LRREFFTTTT KEGYDRRPVD 51 ITPLEQRKLT FDTHALVQDL ETHGFDKTQA ETIVSALTAL SNVSLDTIYK 101 EMVTQAQQEI TVQQLMAHLD AIRKDMVILE KSEFANLRAE NEKMKIELDQ 151 VKQQLMHETΞ RIRADNKLDI NLERSRVTDM FTDQEKQLME TTTEFTKKDT 201 QTKSIISETS NKIDAEIASL KTLMESNKLE TIRYLAASVF TCLAIALGFY 251 RFWK BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_19f19, frame 3 SWISSPROT :YAN8_SCHPO HYPOTHETICAL 24.6 KD PROTEIN C3H1.08 IN CHROMOSOME I., N = 1, Score = 144, P = 8.4e-09 PIR:S56209 probable membrane protein YFL046w - yeast (Saccharomyces cerevisiae), N = 1, Score = 138, P = 5.4e-08 >SWISSPROT:YAN8_ΞCHPO HYPOTHETICAL 24.6 KD PROTEIN C3H1.08 IN CHROMOSOME I. Length = 211 HSPs: Score = 144 (21.6 bits), Expect = 8.4e-09, P = 8.4e-09 Identities = 34/121 (28%), Positives = 67/121 (55%) Query: 70 LETHGFDKTQAETIVSALTALSNVSLDTIYKEMVTQAQQE-ITVQQLMAHLDAIRKDMVI 128 LE G+ AETI + + ++ +L + K + +A+QE ++ QQ L IRK + Sbjct: 46 LEQAGYSVKNAETITNLMRTITGEALTELEKNIGFKAKQESVSFQQKRTFLQ-IRKYLET 104 Query: 129 LEKSEFANLRAENEKMKIELDQVKQQLMHETSRIRADNKLDINLERSRVTDMFTDQEKQL 188 +E++EF +R ++K+ E+++ K L + ++ +L++NLE+ R+ D T + + Sbjct: 105 IEENEFDKVRKSSDKLINEIEKTKSSLREDVKTALSEVRLNLNLEKGRMKDAATSRNTNI 164 Query: 189 ME 190 E Sbjct: 165 HE 166 Pedant information for DKFZphtes3_19f19, frame 3 Report for DKFZphtes3_19f19.3 [LENGTH] 254 [MW] 29505.73 [pi] 6.99 [HOMOL] PIR:S56209 probable membrane protein YFL046w - yeast (Saccharomyces cerevisiae) 2e-10 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YFL046w] 8e-12 [PROSITE] RGD 1 [KW] TRANSMEMBRANE 1 [KW] LOW_COMPLEXITY 5.12 % [KW] COILED COIL 11.02 % SEQ MNSRQAWRLFLSQGRGDRWVΞRPRGHFSPALRREFFTTTTKEGYDRRPVDITPLEQRKLT SEG PRD ccchhhhhhhhhccccceeeeccccccchhhhhhheeeeccccccccccccchhhhhhcc COILS MEM SEQ FDTHALVQDLETHGFDKTQAETIVSALTALSNVSLDTIYKEMVTQAQQEITVQQLMAHLD SEG PRD chhhhhhhhhhhcccccchhhhhhhhhhhhhhchhhhhhhhhhhhhhhhhhhhhhhhhhh COILS MEM SEQ AIRKDMVILEKSEFANLRAENEKMKIELDQVKQQLMHETSRIRADNKLDINLERSRVTDM SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCC MEM SEQ FTDQEKQLMETTTEFTKKDTQTKSIISETSNKIDAEIASLKTLMESNKLETIRYLAASVF SEG xxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhcccccccceeeeehhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS MEM MMMMMMM SEQ TCLAIALGFYRFWK SEG PRD hhhhhhhhhhhccc COILS MEM MMMMMMMMMM.... Prosite for DKFZphtes3_19f19.3 PS00016 15->18 RGD PDOC00016 (No Pfam data available for DKFZphtes3_19f19.3) DKFZphtes3_19jl7 group: testes derived DKFZphtes3_19jl7 encodes a novel 436 amino acid protein with partial similarity to C. elegans Y40B1A.2 protein. The novel protein contains two Prosite WW/rsp5/WWP domain signatures. The WW domain (or rsp5 or WWP domain) has been originally discovered as a short conserved region in a number of unrelated proteins, such as dystrophin, utrophin, vertebrate YAP protein, mouse NEDD-4 and yeast RSP5. The domain is repeated up to 4 times in some proteins. It has been shown to bind proteins with particular prolme-motifs, [AP] -P-P- [AP] -Y, and thus resembles somewhat SH3 domains. It appears to contain beta-strands grouped around four conserved aromatic positions; generally Trp. The name WW or WWP derives from the presence of these Trp as well as that of a conserved Pro. It is frequently associated with other domains typical for proteins in signal transduction processes. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . similarity to C. elegans Y40B1A.2 there are two long ORFs in this cDNA according to EST: HS12146/HΞ75086/AA923755/MMAA17335 remaining intron at Bp 1506-1733 Sequenced by MediGenomix Locus : unknown Insert length: 2762 bp Poly A stretch at pos. 2740, no polyadenylation signal found 1 ATTCTCAGCC AAATTTTTTT ATTTTTTGCA GAATCAGTGT GCAAGGTGGT 51 TTATAAGATA ATGGAGTGGT TTTTTTTTGT GTTTAGTGTG ATTTGTTATC 101 AGGAGTCTTA TTGTAACGCT TAAGCATTAG GTTTTTTGTC TGAGAAACTT 151 TAAAGAGTAA AGCAGAATTG AAAGTGGAAA TTTTAATTTT GTAAGTTCAT 201 AAAATTTAAT GATAATACAC CAAAGTTTAT GTTTAAATTA GGGAGTTTAA 251 GGTTTCAATT CTTTCTCTTT TTTTTTGGGG GGGTGATGTT TTACAGGCAC 301 TTAAGTATTC ATCGAAGAGT CACCCCAGTA GCGGTGATCA CAGACATGAA 351 AAGATGCGAG ACGCCGGAGA TCCTTCACCA CCAAATAAAA TGTTGCGGAG 401 ATCTGATAGT CCTGAAAACA AATACAGTGA CAGCACAGGT CACAGTAAGG 451 CCAAAAATGT GCATACTCAC AGAGTTAGAG AGAGGGATGG TGGGACCAGT 501 TACTCTCCAC AAGAAAATTC ACACAACCAC AGTGCTCTTC ATAGTTCAAA 551 TTCACATTCT TCTAATCCAA GCAATAACCC AAGCAAAACT TCAGATGCAC 601 CTTATGATTC TGCAGATGAC TGGTCTGAGC ATATTAGCTC TTCTGGGAAA 651 AAGTACTACT ACAATTGTCG AACAGAAGTT TCACAATGGG AAAAACCAAA 701 AGAGTGGCTT GAAAGAGAAC AGAGACAAAA AGAAGCAAAC AAGATGGCAG 751 TCAACAGCTT CCCAAAAGAT AGGGATTACA GAAGAGAGGT GATGCAAGCA 801 ACAGCCACTA GTGGGTTTGC CAGTGGAATG GAAGACAAGC ATTCCAGTGA 851 TGCCAGTAGT TTGCTCCCAC AGAATATTTT GTCTCAAACA AGCAGACACA 901 ATGACAGAGA CTACAGACTG CCAAGAGCAG AGACTCACAG TAGTTCTACG 951 CCAGTACAGC ACCCCATCAA ACCAGTGGTT CATCCAACTG CTACCCCAAG 1001 CACTGTTCCT TCTAGTCCAT TTACGCTACA GTCTGATCAC CAGCCAAAGA 1051 AATCATTTGA TGCTAATGGA GCATCTACTT TATCAAAACT GCCTACACCC 1101 ACATCTTCTG TCCCTGCACA GAAAACAGAA AGAAAAGAAT CTACATCAGG 1151 AGACAAACCC GTATCACATT CTTGCACAAC TCCTTCCACG TCTTCTGCCT 1201 CTGGACTGAA CCCCACATCT GCACCTCCAA CATCTGCTTC AGCGGTCCCT 1251 GTTTCTCCTG TTCCACAGTC GCCAATACCT CCCTTACTTC AGGACCCAAA 1301 TCTTCTTAGA CAATTGCTTC CTGCTTTGCA AGCCACGCTG CAGCTTAATA 1351 ATTCTAATGT GGACATATCT AAAATAAATG AAGTTCTTAC AGCAGCTGTG 1401 ACACAAGCCT CACTGCAGTC TATAATTCAT AAGTTTCTTA CTGCTGGACC 1451 ATCTGCTTTC AACATAACGT CTCTGATTTC TCAAGCTGCT CAGCTCTCTA 1501 CACAAGATAT CCCTCTTCAT GAAGGTATCC AAATGGAGAG AGATACACAT 1551 AGGAGCAAAT GGGAAGTGAA AGGGTCACTT TGTCAGAAAG CTGATAAACA 1601 GCAGGAATGC CTTGTCTGGA ATGGAAGTAT AATGGTGCAA AGACTCTTGC 1651 AACCCTCTGG CTAGCCTCAT GAGCAGGAGA CTGCGTGGGA TACCTGGGCC 1701 TAAATGTAGA ATAAGAAAGA AGAAATAAGG ATGCCCAGCC ATCTAATCAG 1751 TCTCCGATGT CTTTAACATC TGATGCGTCA TCCCCAAGAT CATATGTTTC 1801 TCCAAGAATA AGCACACCTC AAACTAACAC AGTCCCTATC AAACCTTTGA 1851 TCAGTACTCC TCCTGTTTCA TCACAGCCAA AGGTTAGTAC TCCAGTAGTT 1901 AAGCAAGGAC CAGTGTCACA GTCAGCCACA CAGCAGCCTG TAACTGCTGA 1951 CAAGCAGCAA GGTCATGAAC CTGTCTCTCC TCGAAGTCTT CAGCGCTCAA 2001 GCCAGAGAAG TCCATCACCT GGTCCCAATC ATACTTCTAA TAGTAGTAAT 2051 GCATCAAATG CAACAGTTGT ACCACAGAAT TCTTCTGCCC GATCCACGTG 2101 TTCATTAACG CCTGCACTAG CAGCACACTT CAGTGAAAAT CTCATAAAAC 2151 ACGTTCAAGG ATGGCCTGCA GATCATGCAG AGAAGCAGGC ATCAAGATTA 2201 CGCGAAGAAG CGCATAACAT GGGAACTATT CACATGTCCG AAATTTGTAC 2251 TGAATTAAAA AATTTAAGAT CTTTAGTCCG AGTATGTGAA ATTCAAGCAA 2301 CTTTGCGAGA GCAAAGGATA CTATTTTTGA GACAACAAAT TAAGGAACTT 2351 GAAAAGCTAA AAAATCAGAA TTCCTTCATG GTGTGAAGAT GTGAATAATT 2401 GCACATGGTT TTGAGAACAG GAACTGTAAA TCTGTTGCCC AATCTTAACA 2451 TTTTTGAGCT GCATTTAAGT AGACTTTGGA CCGTTAAGCT GGGCAAAGGA 2501 AATGACAAGG GGACGGGGTC TGTGAGAGTC AATTCAGGGG AAAGATACAA 2551 GATTGATTTG TAAAACCCTT GAAATGTAGA TTTCTTGTAG ATGTATCCTT 2601 CACGTTGTAA ATATGTTTTG TAGAGTGAAG CCATGGGAAG CCATGTGTAA 2651 CAGAGCTTAG ACATCCAAAA CTAATCAATG CTGAGGTGGC TAAATACCTA 2701 GCCTTTTACA TGTAAACCTG TCTGCAAAAT TAGCTTTTTT AAAAAAAAAA 2751 AAAAAAAAAA AA BLAST Results Entry AC005876 from database EMBLNEW: Homo sapiens chromosome 10 clone CIT987ΞK-1188I5 map lOpll .2-10pl2.1, complete sequence. Score = 2130, P = O.Oe+00, identities = 426/426 12 exons matching Bp 492-2740 Medline entries No Medlme entry Peptide information for frame 2 ORF from 1757 bp to 2383 bp; peptide length: 209 Category: questionable ORF Classification: no clue 1 MSLTSDASSP RSYVSPRIST PQTNTVPIKP LISTPPVSSQ PKVSTPVVKQ 51 GPVSQSATQQ PVTADKQQGH EPVSPRSLQR SSQRSPSPGP NHTSNSSNAS 101 NATVVPQNSS ARSTCSLTPA LAAHFSENLI KHVQGWPADH AEKQASRLRE 151 EAHNMGTIHM SEICTELKNL RSLVRVCEIQ ATLREQRILF LRQQIKELEK 201 LKNQNSFMV BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_19j 17, frame 2 No Alert BLASTP hits found Peptide information for frame 3 ORF from 354 bp to 1661 bp; peptide length: 436 Category: similarity to unknown protein Classification: unclassified Prosite motifs: WW_DOMAIN_l (90-116) WW DOMAIN 1 (90-116) 1 MRDAGDPSPP NKMLRRSDSP ENKYSDSTGH SKAKNVHTHR VRERDGGTSY 51 SPQENSHNHS ALHSSNSHSS NPSNNPSKTS DAPYDSADDW SEHISSSGKK 101 YYYNCRTEVS QWEKPKEWLE REQRQKEANK MAVNSFPKDR DYRREVMQAT 151 ATSGFASGME DKHSSDASSL LPQNILSQTS RHNDRDYRLP RAETHSSSTP 201 VQHPIKPVVH PTATPSTVPS SPFTLQSDHQ PKKSFDANGA STLSKLPTPT 251 SSVPAQKTER KESTSGDKPV SHSCTTPSTS SASGLNPTSA PPTSASAVPV 301 SPVPQSPIPP LLQDPNLLRQ LLPALQATLQ LNNSNVDISK INEVLTAAVT 351 QASLQSIIHK FLTAGPSAFN ITSLISQAAQ LSTQDIPLHE GIQMERDTHR 401 SKWEVKGSLC QKADKQQECL VWNGSIMVQR LLQPSG BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_19j 17, frame 3 TREMBL :CEY40B1A_2 gene: "Y40B1A.2"; Caenorhabditis elegans cosmid Y40B1A, N = 1, Score = 144, P = 1.8e-09 >TREMBL:CEY40B1A_2 gene: "Y40B1A.2"; Caenorhabditis elegans cosmid Y40B1A Length = 120 HSPs: Score = 144 (21.6 bits), Expect = 1.8e-09, P = 1.8e-09 Identities = 30/67 (44%), Positives = 43/67 (64%) Query: 90 WSEHISSSGKKYYYNCRTEVSQWEKPKEW-LEREQRQKEANKMAVNSFPK DRDYRRE 145 W+E +SSSGK YYYN +TE+SQW+KP EW E +++ K VN P+ DR Y Sbjct: 11 WTEQMSSSGKMYYYNKKTEISQWDKPAEWPAEGGSAERDKPKGGVNEKPRFAEDR-YNEY 69 Query: 146 VMQATATS 153 + Q +++S Sbjct: 70 IGQLΞSSS 77 Pedant information for DKFZphtes3_19j 17, frame 2 Report for DKFZphtes3_19jl7.2 [LENGTH] 209 [MW] 22873.85 [pi] 9.95 [KW] All_Alpha [KW] LOW_COMPLEXITY 13.40 % SEQ MSLTSDASSPRSYVSPRISTPQTNTVPIKPLISTPPVSSQPKVSTPVVKQGPVSQSATQQ SEG PRD ccccccccccccccccccccccceeeeccccccccccccccccccceeeccccccccccc SEQ PVTADKQQGHEPVSPRSLQRΞSQRSPSPGPNHTSNSSNASNATVVPQNSSARSTCSLTPA SEG xxxxxxxxxxxxxxx..xxxxxxxxxxxxx PRD cccccccccccccccccccccccccccccccccccccccccceeeeccccccccccchhh SEQ LAAHFSENLIKHVQGWPADHAEKQASRLREEAHNMGTIHMSEICTELKNLRSLVRVCEIQ SEG PRD hhhhhhcchhhhhhccccchhhhhhhhhhhhhhhcccchhhhhhhhhhhhhhhhhhhhhh SEQ ATLREQRILFLRQQIKELEKLKNQNSFMV SEG PRD hhhhhhhhhhhhhhhhhhhhhhhcccccc (No Prosite data available for DKFZphtes3_19j 17.2) (No Pfam data available for DKFZphtes3_19j 17.2) Pedant information for DKFZphtes3_19j 17, frame 3 Report for DKFZphtes3_19 l7.3 [LENGTH] 436 [MW] 47716.62 [pi] 8.71 [HOMOL] TREMBL:CEY40B1A_2 gene: "Y40B1A.2"; Caenorhabditis elegans cosmid Y40B1A 6e-0S [FUNCAT] 04.05.03 mrna processing (splicing) [Ξ. cerevisiae, YKL012w] 2e-04 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YKL012w] 2e-04 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YPR152c] 6e-04 [BLOCKS] BL01159 WW/rsp5/WWP domain proteins [PROSITE] WW_DOMAIN_l 2 [PFAM] WW/rsp5/WWP domain containing proteins [KW] All_Alpha [KW] LOW COMPLEXITY 22.48 % SEQ MRDAGDPSPPNKMLRRSDSPENKYSDSTGHSKAKNVHTHRVRERDGGTSYSPQENSHNHS SEG xxxxxx PRD ccccccccccccccccccccccccccccccccccccceeeeeeccccccccccccccccc SEQ ALHSSNSHSSNPSNNPSKTSDAPYDSADDWSEHISSSGKKYYYNCRTEVSQWEKPKEWLE SEG xxxxxxxxxxxxxxxxx PRD ccccccccccccccccccccccccccccccceeeccccceeeeeeccccccccccchhhh SEQ REQRQKEANKMAVNSFPKDRDYRREVMQATATSGFASGMEDKHSSDASSLLPQNILSQTS SEG PRD hhhhhhhhhhhhcccccccchhhhhhhhhhcccccccccccccccccccccccccccccc SEQ RHNDRDYRLPRAETHSSSTPVQHPIKPVVHPTATPSTVPSSPFTLQSDHQPKKSFDANGA SEG xxxxxxxxxxxxxxxx PRD ccccccccccccccccccccccccceeeeccccccccccccccccccccccccccccccc SEQ STLSKLPTPTSSVPAQKTERKESTSGDKPVSHSCTTPSTSSASGLNPTSAPPTSASAVPV SEG xxxxxxxxxxxx xxxxxxxxxxxxxx PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ SPVPQSPIPPLLQDPNLLRQLLPALQATLQLNNSNVDIΞKINEVLTAAVTQASLQSIIHK SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD cccccccccccccccchhhhhhhhhhhhhhhcccccccccchhhhhhhhhhhhhhhhhhh SEQ FLTAGPSAFNITSLISQAAQLΞTQDIPLHEGIQMERDTHRSKWEVKGSLCQKADKQQECL SEG PRD hhcccccceeehhhhhhhhhhhccccccccccccccccccceeeecccchhhhhhhccee SEQ VWNGSIMVQRLLQPSG SEG PRD eeccchhhhhhccccc Prosite for DKFZphtes3_19j 17.3 PS01159 90->116 WW_DOMAIN_l PDOC50020 PS01159 90->116 WW DOMAIN 1 PDOC50020 Pfam for DKFZphtes3_19j 17.3 HMM_NAME WW/rsp5/WWP domain containing proteins HMM *LPsGWEeHWDpsGRpWYYWNHETkTTQWEpP* + ++W EH++ SG+ YY+N T+ +QWE+P Query 86 SADDWSEHISSSGKK-YYYNCRTEVΞQWEKP 115 DKFZphtes3_lcl group : signal transduction DKFZphtes3_lcl encodes a novel 632 amino acid putative GTPase-activating protein, related to drosophila rotund transcript and human n-chimaerin . rac small GTPase is associated with type-I phosphatidylinositol 4-phosphate 5-kιnase and regulating the production of phosphatidylinositol 4 , 5-bιsphosphate . The new protein is expected to activate p21rac-related small GTPases . The new protein can find application in modulating/blocking the response to a cellular receptor . similarity to GTPase-activating proteins complete cDNA, complete eds , EST hits Sequenced by DKFZ Locus : unknown Insert length : 3237 bp Poly A stretch at pos . 3227 , no polyadenylation signal found 1 GCGAAGTGAA GGGTGGCCCA GGTGGGGCCA GGCTGACTGA ATGTATCTCC 51 TAGCTATGGA CTAAATAATA CATGGGGGGA AATAAACAAG TATTCATGAG 101 GGTGAAAATG TGACCCAGCA GGAAAATTAC AACTATTTTC AATTGACGTT 151 GAATAGGATG AGTCATGGAA TTTAAGTGAT TTACTGAAGA TTATACTACT 201 GGTAGATAGA AGAGCTAAAG AAAGATGGAT ACTATGATGC TGAATGTGCG 251 GAATCTGTTT GAGCAGCTTG TGCGCCGGGT GGAGATTCTC AGTGAAGGAA 301 ATGAAGTCCA ATTTATCCAG TTGGCGAAGG ACTTTGAGGA TTTCCGTAAA 351 AAGTGGCAGA GGACTGACCA TGAGCTGGGG AAATACAAGG ATCTTTTGAT 401 GAAAGCAGAG ACTGAGCGAA GTGCTCTGGA TGTTAAGCTG AAGCATGCAC 451 GTAATCAGGT GGATGTAGAG ATCAAACGGA GACAGAGAGC TGAGGCTGAC 501 TGCGAAAAGC TGGAACGACA GATTCAGCTG ATTCGAGAGA TGCTCATGTG 551 TGACACATCT GGCAGCATTC AACTAAGCGA GGAGCAAAAA TCAGCTCTGG 601 CTTTTCTCAA CAGAGGCCAA CCATCCAGCA GCAATGCTGG GAACAAAAGA 651 CTATCAACCA TTGATGAATC TGGTTCCATT TTATCAGATA TCAGCTTTGA 701 CAAGACTGAT GAATCACTGG ATTGGGACTC TTCTTTGGTG AAGACTTTCA 751 AACTGAAGAA GAGAGAAAAG AGGCGCTCTA CTAGCCGACA GTTTGTTGAT 801 GGTCCCCCTG GACCTGTAAA GAAAACTCGT TCCATTGGCT CTGCAGTAGA 851 CCAGGGGAAT GAATCCATAG TTGCAAAAAC TACAGTGACT GTTCCCAATG 901 ATGGCGGGCC CATCGAAGCT GTGTCCACTA TTGAGACTGT GCCATATTGG 951 ACCAGGAGCC GAAGGAAAAC AGGTACTTTA CAACCTTGGA ACAGTGACTC 1001 CACCCTGAAC AGCAGGCAGC TGGAGCCAAG AACTGAGACA GACAGTGTGG 1051 GCACGCCACA GAGTAATGGA GGGATGCGCC TGCATGACTT TGTTTCTAAG 1101 ACGGTTATTA AACCTGAATC CTGTGTTCCA TGTGGAAAGC GGATAAAATT 1151 TGGCAAATTA TCTCTGAAGT GTCGAGACTG TCGTGTGGTC TCTCATCCAG 1201 AATGTCGGGA CCGCTGTCCC CTTCCCTGCA TTCCTACCCT GATAGGAACA 1251 CCTGTCAAGA TTGGAGAGGG AATGCTGGCA GACTTTGTGT CCCAGACTTC 1301 TCCAATGATC CCCTCCATTG TTGTGCATTG TGTAAATGAG ATTGAGCAAA 1351 GAGGTCTGAC TGAGACAGGC CTGTATAGGA TCTCTGGCTG TGACCGCACA 1401 GTAAAAGAGC TGAAAGAGAA ATTCCTCAGA GTGAAAACTG TACCCCTCCT 1451 CAGCAAAGTG GATGATATCC ATGCTATCTG TAGCCTTCTA AAAGACTTTC 1501 TTCGAAACCT CAAAGAACCT CTTCTGACCT TTCGCCTTAA CAGAGCCTTT 1551 ATGGAAGCAG CAGAAATCAC AGATGAAGAC AACAGCATAG CTGCCATGTA 1601 CCAAGCTGTT GGTGAACTGC CCCAGGCCAA CAGGGACACA TTAGCTTTCC 1651 TCATGATTCA CTTGCAGAGA GTGGCTCAGA GTCCACATAC TAAAATGGAT 1701 GTTGCCAATC TGGCTAAAGT CTTTGGCCCT ACAATAGTGG CCCATGCTGT 1751 GCCCAATCCA GACCCAGTGA CAATGTTACA GGACATCAAG CGTCAACCCA 1801 AGGTGGTTGA GCGCCTGCTT TCCTTGCCTC TGGAGTATTG GAGTCAGTTC 1851 ATGATGGTGG AGCAAGAGAA CATTGACCCC CTACATGTCA TTGAAAACTC 1901 AAATGCCTTT TCAACACCAC AGACACCAGA TATTAAAGTG AGTTTACTGG 1951 GACCTGTGAC CACTCCTGAA CATCAGCTTC TCAAGACTCC TTCATCTAGT 2001 TCCCTGTCAC AGAGAGTCCG TTCCACCCTC ACCAAGAACA CTCCTAGATT 2051 TGGGAGCAAA AGCAAGTCTG CCACTAACCT AGGACGACAA GGCAACTTTT 2101 TTGCTTCTCC AATGCTCAAG TGAAGTCACA TCTGCCTGTT ACTTCCCAGC 2151 ATTGACTGAC TATAAGAAAG GACACATCTG TACTCTGCTC TGCAGCCTCC 2201 TGTACTCATT ACTACTTTTA GCATTCTCCA GGCTTTTACT CAAGTTTAAT 2251 TGTGCATGAG GGTTTTATTA AAACTATATA TATCTCCCCT TCCTTCTCCT 2301 CAAGTCACAT AATATCAGCA CTTTGTGCTG GTCATTGTTG GGAGCTTTTA 2351 GATGAGACAT CTTTCCAGGG GTAGAAGGGT TAGTATGGAA TTGGTTGTGA 2401 TTCTTTTTGG GGAAGGGGGT TATTGTTCCT TTGGCTTAAA GCCAAATGCT 2451 GCTCATAGAA TGATCTTTCT CTAGTTTCAT TTAGAACTGA TTTCCGTGAG 2501 ACAATGACAG AAACCCTACC TATCTGATAA GATTAGCTTG TCTCAGGGTG 2551 GGAAGTGGGA GGGCAGGGCA AAGAAAGGAT TAGACCAGAG GATTTAGGAT 2601 GCCTCCTTCT AAGAACCAGA AGTTCTCATT CCCCATTATG AACTGAGCTA 2651 TAATATGGAG CTTTCATAAA AATGGGATGC ATTGAGGACA GAACTAGTGA 2701 TGGGAGTATG CGTAGCTTTG ATTTGGATGA TTAGGTCTTT AATAGTGTTG 2751 AGTGGCACAA CCTTGTAAAT GTGAAAGTAC AACTCGTATT TATCTCTGAT 2801 GTGCCGCTGG CTGAACTTTG GGTTCATTTG GGGTCAAAGC CAGTTTTTCT 2851 TTTAAAATTG AATTCATTCT GATGCTTGGC CCCCATACCC CCAACCTTGT 2901 CCAGTGGAGC CCAACTTCTA AAGGTCAATA TATCATCCTT TGGCATCCCA 2951 ACTAACAATA AAGAGTAGGC TATAAGGGAA GATTGTCAAT ATTTTGTGGT 3001 AAGAAAAGCT ACAGTCATTT TTTCTTTGCA CTTTGGATGC TGAAATTTTT 3051 CCCATGGAAC ATAGCCACAT CTAGATAGAT GTGAGCTTTT TCTTCTGTTA 3101 AAATTATTCT TAATGTCTGT AAAAACGATT TTCTTCTGTA GAATGTTTGA 3151 CTTCGTATTG ACCCTTATCT GTAAAACACC TATTTGGGAT AATATTTGGA 3201 AAAAAAGTAA ATAGCTTTTT CAAAATGAAA AAAAAAA BLAST Results Entry U82984 from database EMBLEST : Homo sapiens DRES 56 mRNA sequence. Score = 8775, P = O.Oe+00, identities = 1757/1758 matches 3 ' end Medline entries 93074974: Developmental regulation and neuronal expression of the mRNA of rat n-chimaeπn, a p21rac GAP:cDNA sequence. 93024458: A Drosophila rotund transcript expressed during spermatogenesis and imaginal disc morphogenesis encodes a protein which is similar to human Rac GTPase-activating (racGAP) proteins. Peptide information for frame 3 ORF from 225 bp to 2120 bp; peptide length: 632 Category: similarity to known protein 1 MDTMMLNVRN LFEQLVRRVE ILSEGNEVQF IQLAKDFEDF RKKWQRTDHE 51 LGKYKDLLMK AETERSALDV KLKHARNQVD VEIKRRQRAE ADCEKLERQI 101 QLIREMLMCD TSGSIQLSEE QKSALAFLNR GQPSSSNAGN KRLSTIDESG 151 SILSDISFDK TDESLDWDSS LVKTFKLKKR EKRRSTSRQF VDGPPGPVKK 201 TRSIGSAVDQ GNESIVAKTT VTVPNDGGPI EAVSTIETVP YWTRSRRKTG 251 TLQPWNSDST LNSRQLEPRT ETDSVGTPQS NGGMRLHDFV SKTVIKPESC 301 VPCGKRIKFG KLSLKCRDCR VVSHPECRDR CPLPCIPTLI GTPVKIGEGM 351 LADFVSQTSP MIPSIVVHCV NEIEQRGLTE TGLYRISGCD RTVKELKEKF 401 LRVKTVPLLS KVDDIHAICS LLKDFLRNLK EPLLTFRLNR AFMEAAEITD 451 EDNSIAAMYQ AVGELPQANR DTLAFLMIHL QRVAQSPHTK MDVANLAKVF 501 GPTIVAHAVP NPDPVTMLQD IKRQPKVVER LLSLPLEYWS QFMMVEQENI 551 DPLHVIENSN AFSTPQTPDI KVSLLGPVTT PEHQLLKTPS SSSLSQRVRS 601 TLTKNTPRFG SKSKSATNLG RQGNFFASPM LK BLASTP hits Entry CEK08E3_4 from database TREMBLNEW: gene: "K08E3.6"; Caenorhabditis elegans cosmid K08E3 Score = 452, P = 2.6e-48, identities = 126/377, positives = 189/377 Entry A48122 from database PIR: GTPase-activatmg protein Rac homolog, splice form clone pel.7 - fruit fly (Drosophila melanogaster) (fragment) Score = 480, P = 9.2e-46, identities = 111/270, positives = 155/270 Entry B48122 from database PIR: GTPase-activat g protein Rac homolog, splice form clone pcl.7d - fruit fly (Drosophila melanogaster) Score = 480, P = 9.2e-46, identities = 111/270, positives = 155/270 Entry DM22539_1 from database TREMBL: gene: "rotund"; product: "rnracGAP"; Drosophila melanogaster rnracGAP (rotund) gene, complete eds. Score = 480, P = 9.2e-46, identities = 111/270, positives = 155/270 Entry S29128 from database PIR: N-chimerm - rat Score = 336, P = 8.8e-30, identities = 86/253, positives 128/253 Alert BLASTP hits for DKFZphtes3_lcl, frame 3 No Alert BLASTP hits found Pedant information for DKFZphtes3_lcl, frame 3 Report for DKFZphtes3_lcl .3 [LENGTH] 632 [MW] 71026.84 [pi] 9.08 [HOMOL] PIR:B48122 GTPase-activatmg protein Rac homolog, splice form clone pcl.7d - fruit fly (Drosophila melanogaster) 2e-46 [FUNCAT] 10.99 other signal-transduction activities [S. cerevisiae, YBR260C] 3e-12 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YER155C] 2e-ll [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YER155C] 2e-ll [FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YER155c] 2e-ll [FUNCAT] 03.10 sporulation and germination [S. cerevisiae, YDL240w] 3e-09 [FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YOR134w] 4e-09 [ FUNCAT ] 06.10 assembly of protein complexes [S. cerevisiae, YOR134w] 4e-09 [FUNCAT] 03.07 pheromone response, mating-type determination, sex-specific proteins [S. cerevisiae, YOR127w] 5e-09 [FUNCAT] 09.04 biogenesis of cytoskeleton [S. cerevisiae, YPL115C] 3e-08 [FUNCAT] 10.02.09 regulation of g-protein activity [S. cerevisiae, YPL115c] 3e-08 [BLOCKS] BL00479B Phorbol esters / diacylglycerol binding domain proteins [BLOCKS] BL00479A Phorbol esters / diacylglycerol binding domain proteins [SCOP] dlpbwa_ 1.83.1.1.2 p85 alpha subunit RhoGAP domain [human (Hom le-55 [SCOP] dlrgp 1.83.1.1.1 p50 RhoGAP domain human (Homo sapiens) le-49 [PIRKW] breakpoint cluster region le-19 [PIRKW] transmembrane protein 7e-08 [PIRKW] brain 3e-22 [PIRKW] alternative splicing le-19 [PIRKW] P-loop 2e-25 [SUPFAM] CDC24 homology 3e-22 [SUPFAM] bcr protein 3e-22 [SUPFAM] myosin motor domain homology 2e-25 [SUPFAM] pleckstrm repeat homology 4e-10 [SUPFAM] LIM metal-b dmg repeat homology 2e- 09 [SUPFAM] protem kinase C zmc-binding repeat homology 5e-29 [PROSITE] MYRISTYL 6 [PROSITE] AMIDATION 1 [PROSITE] CAMP_PHOSPHO_SITE 3 [PROSITE] CK2_PHOSPHO_SITE 13 [PROSITE] TYR_PHOSPHO_SITE 2 [PROSITE] PKC_PHOSPHO_SI E 9 [PROSITE] ASN_GLYCOSYLATION 1 [PROSITE] DAG PE BINDING DOMAIN 1 [PFAM] Phorbol esters / diacylglycerol binding domain [KW] Irregular [KW] 3D [KW] LOW_COMPLEXITY 2.22 % [KW] COILED COIL 8.54 % SEQ MDTMMLNVRNLFEQLVRRVEILSEGNEVQFIQLAKDFEDFRKKWQRTDHELGKYKDLLMK SEG COILS cccccccccccc Irgp- SEQ AETERSALDVKLKHARNQVDVEIKRRQRAEADCEKLERQIQLIREMLMCDTSGSIQLΞEE SEG COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC Irgp- SEQ QKSALAFLNRGQPSSSNAGNKRLSTIDESGSILΞDISFDKTDESLDWDSSLVKTFKLKKR SEG COILS irgp- SEQ EKRRSTSRQFVDGPPGPVKKTRΞIGSAVDQGNEΞIVAKTTVTVPNDGGPIEAVSTIETVP SEG COILS irgp- SEQ YWTRΞRRKTGTLQPWNSDSTLNSRQLEPRTETDSVGTPQSNGGMRLHDFVSKTVIKPESC SEG COILS Irgp- SEQ VPCGKRIKFGKLSLKCRDCRVVSHPECRDRCPLPCIPTLIGTPVKIGEGMLADFVSQTSP SEG COILS irgp- SEQ MIPSIVVHCVNEIEQRGLTETGLYRISGCDRTVKELKEKFLRVKTVPLLSKVDDIHAICS SEG COILS Irgp- .CCHHHHHHHHHHHHHHTTTTTTTTTCCCHHHHHHHHHHHHHCCCCCG-GGCCCCHHHHH SEQ LLKDFLRNLKEPLLTFRLNRAFMEAAEITDEDNSIAAMYQAVGELPQANRDTLAFLMIHL SEG COILS Irgp- HHHHHHHHTTTTTTTGGGHHHHHHTTTT-CGGGHHHHHHHHHHHCCHHHHHHHHHHHHHH SEQ QRVAQSPHTKMDVANLAKVFGPTIVAHAVPNPDPVTMLQDIKRQPKVVERLLSLPLEYWS SEG COILS Irgp- HHHHHHHHHCCCHHHHHHHHGGGCC SEQ QFMMVEQENIDPLHVIENSNAFSTPQTPDIKVSLLGPVTTPEHQLLKTPSSSSLSQRVRS SEG xxxxxxxxxxx COILS irgp- SEQ TLTKNTPRFGSKSKSATNLGRQGNFFASPMLK SEG xxx COILS irgp- Prosite for DKFZphtes3_lcl .3 PS00001 212->216 ASN_GLYCOSYLATION PDOC00001 PS00004 141->145 CAMP_PHOSPHO_SITE PDOC00004 PS00004 182->186 CAMP_PHOSPHO_SITE PDOC00004 PS00004 246->250 CAMP_PHOSPHO_SITE PDOC00004 PS00005 63->66 PKC_PHOSPHO_SITE PDOC00005 PS00005 174->177 PKC_PHOSPHO_SITE PDOC00005 PS00005 186->189 PKC_PHOSPHO_SITE PDOC00005 PS00005 245->248 PKC_PHOSPHO_SITE PDOC00005 PS00005 313->316 PKC_PHOSPHO_SITE PDOC00005 PS00005 392->395 PKC_PHOSPHO_SITE PDOC00005 PS00005 435->438 PKC_PHOSPHO_SITE PDOC00005 PS00005 595->598 PKC_PHOSPHO_SITE PDOC00005 PS00005 606->609 PKC_PHOSPHO_SITE PDOC00005 PS00006 47->51 CK2_PHOSPHO_SITE PDOC00006 PS00006 66->70 CK2_PHOSPHO_SITE PDOC00006 PS00006 144->148 CK2_PHOΞPHO_SITE PDOC00006 PS00006 206->210 CK2_PHOSPHO_SITE PDOC00006 PS00006 234->238 CK2_PHOSPHO_SITE PDOC00006 PS00006 270->274 CK2_PHOSPHO_SITE PDOC00006 PS00006 323->327 CK2_PHOSPHO_SITE PDOC00006 PS00006 387->391 CK2_PHOSPHO_SITE PDOC00006 PS00006 392->396 CK2_PHOSPHO_SITE PDOC00006 PS00006 410->414 CK2_PHOSPHO_SITE PDOC00006 PS00006 449->453 CK2_PHOSPHO_SITE PDOC00006 PS00006 489->493 CK2_PHOSPHO_SITE PDOC00006 PS00006 579->583 CK2_PHOSPHO_SITE PDOC00006 PS00007 46->55 TYR_PHOSPHO_SITE PDOC00007 PS00007 376->385 TYR_PHOSPHO_SITE PDOC00007 PS00008 131->137 MYRISTYL PDOC00008 PS00008 150->156 MYRISTYL PDOC00008 PS00008 276->282 MYRISTYL PDOC00008 PS00008 377->383 MYRISTYL PDOC00008 PS00008 388->394 MYRISTYL PDOC00008 PS00008 623->629 MYRISTYL PDOC00008 PS00009 303->307 AMIDATION PDOC00009 PS00479 287->336 DAG PE BINDING DOMAIN PDOC00379 Pfam for DKFZphtes3_lcl .3 HMM_NAME Phorbol esters / diacylglycerol binding domain HMM *HrFmrHTFrqPTWCDHCgeFIWGWgKQGYQCQnCgMNCHKRCHelVPmm H+F+ +T + P +C CG +1 +GK ++C +C+++ H +C+ + P Query 287 HDFVSKTVIKPESCVPCGKRI-KFGKLSLKCRDCRVVSHPECRDRCPLP 334 HMM C* C Query 335 C 335 DKFZphtes3_lgl3 group: intracellular transport and trafficking DKFZp DKFZphtes3_lgl3 encodes a novel 1007 amino acid protein with similarity to human 256 kD golgin. The new protein contains 7 leucme zippers and seems to be involved in protein-protein- mteraction in the golgi apparatus. The very similar rat cpl51 shows haploid-specific transcription in mus musculus testis. The new protein can find application m modulating protein traffic in the golgi apparatus, especially in human haploid germ cells. similarity to 256 kD golgi, strong similarity to rat "cpl51" 21 exons encoded on AC004682 EST from a testis library, two mouse ESTs of a testis cDNA library, rat cpl51 shows haploid-specific transcription 1 testis or haploid-specific transcription Sequenced by DKFZ Locus: map="16q22.2" Insert length: 3405 bp Poly A stretch at pos. 3394, polyadenylation signal at pos. 3373 1 GGGATAGGGG ATGTGGTTTG TTACAAAGGA TGAGTATTTT GATAGCTTCT 51 CATTCCTTGA ACTATTCTGC AGGTTTATAA CAAAGCTCAG AAAATACTAA 101 AGGTTAAAGG AGAATTGAGA GCTGCCAAGG AAATGAAAGA TGAGGCGGGG 151 GAGAGAGACA GAGAAGTGAG CAGCCTGAAC AGCAAGCTGT TAAGCCTGCA 201 ACTTGACATC AAGAATCTGC ACGATGTCTG CAAGAGACAG AGGAAGACCT 251 TGCAGGACAA TCAGCTCTGC ATGGAGGAGG CAATGAACAG CAGCCACGAC 301 AAGAAGCAAG CACAGGCATT AGCATTCGAG GAGTCAGAGG TGGAATTTGG 351 GTCCAGTAAA CAGTGTCATC TGAGACAACT CCAGCAACTG AAGAAAAAAT 401 TGCTGGTCCT TCAACAAGAA CTGGAGTTTC ACACAGAGGA GTTGCAGACT 451 TCTTACTATT CTCTCCGCCA GTATCAGTCC ATCCTAGAGA AGCAGACTTC 501 CGACCTGGTT CTTCTGCACC ATCACTGCAA ACTGAAAGAA GATGAGGTGA 551 TTCTCTATGA GGAGGAAATG GGAAATCACA ACGAGAACAC AGGGGAGAAG 601 CTCCATTTGG CGCAGGAGCA ACTCGCCTTG GCCGGGGACA AGATCGCCTC 651 TCTAGAGAGG AGCTTAAACC TCTACAGGGA TAAATACCAG TCTTCCCTGA 701 GCAACATCGA GTTACTAGAA TGCCAAGTGA AGATGTTGCA GGGGGAACTC 751 GGCGGGATCA TGGGTCAGGA GCCTGAGAAC AAGGGTGATC ATTCAAAGGT 801 ACGGATATAC ACTTCTCCTT GCATGATTCA AGAGCATCAG GAGACTCAGA 851 AACGACTGTC TGAAGTCTGG CAAAAGGTCT CTCAACAGGA TGATCTCATT 901 CAAGAACTTC GAAATAAGCT GGCCTGCAGT AACGCTTTGG TTCTGGAGCG 951 TGAAAAGGCT TTGATAAAAC TACAAGCCGA TTTTGCTTCC TGTACAGCCA 1001 CCCACAGATA CCCTCCTAGC TCCTCAGAAG AGTGTGAAGA CATCAAAAAG 1051 ATACTGAAGC ACTTGCAGGA GCAGAAAGAC AGCCAGTGCC TGCATGTGGA 1101 GGAGTACCAG AACCTGGTGA AGGATCTGCG CGTGGAACTA GAGGCCGTGT 1151 CGGAACAGAA GAGAAACATC ATGAAGGACA TGATGAAGCT GGAGCTGGAC 1201 CTGCACGGAC TGCGGGAGGA GACATCTGCC CACATTGAGA GGAAGGATAA 1251 GGACATCACC ATCCTGCAGT GCCGGCTGCA GGAGCTGCAG CTGGAGTTCA 1301 CCGAGACCCA AAAGCTCACT TTGAAGAAAG ACAAGTTCCT CCAAGAGAAA 1351 GATGAGATGC TGCAAGAGCT GGAGAAGAAA CTGACACAGG TTCAGAACAG 1401 CCTCCTGAAA AAGGAGAAGG AGCTGGAGAA GCAGCAGTGC ATGGCCACAG 1451 AACTTGAAAT GACAGTCAAG GAGGCTAAGC AGGACAAGTC CAAGGAGGCG 1501 GAGTGCAAGG CCCTGCAGGC TGAGGTCCAG AAGCTGAAGA ACAGTCTCGA 1551 AGAGGCCAAG CAGCAGGAGA GGCTGGCTGC TCAGCAAGCA GCCCAGTGCA 1601 AAGAAGAGGC TGCACTGGCA GGCTGTCACC TGGAGGACAC CCAGAGGAAA 1651 CTGCAGAAGG GTCTCCTCCT GGACAAGCAG AAGGCAGACA CCATCCAGGA 1701 ACTACAGAGA GAACTTCAGA TGCTGCAGAA GGAGTCCTCG ATGGCTGAGA 1751 AGGAACAAAC CTCCAACAGA AAACGGGTGG AGGAGCTGTC ATTAGAACTC 1801 TCTGAAGCCC TGAGGAAGCT TGAAAATTCA GACAAGGAAA AGAGGCAGCT 1851 TCAGAAGACA GTGGCTGAGC AGGATATGAA AATGAATGAC ATGCTTGATC 1901 GTATCAAGCA CCAGCACAGG GAGCAAGGCT CCATCAAATG CAAGTTAGAA 1951 GAAGATCTTC AGGAGGCCAC AAAGCTTCTG GAGGACAAAC GGGAGCAGTT 2001 GAAGAAGAGC AAAGAGCATG AGAAGCTGAT GGAGGGAGAA CTTGAAGCTT 2051 TGCGGCAGGA ATTTAAAAAG AAAGACAAGA CGTTGAAAGA GAATTCCAGA 2101 AAGTTGGAGG AAGAAAATGA GAATCTCCGA GCAGAGCTAC AGTGTTGTTC 2151 TACACAACTG GAATCCTCTC TCAACAAATA CAACACCAGC CAGCAAGTCA 2201 TCCAAGACTT GAATAAAGAG ATAGCCCTTC AGAAGGAGTC CTTAATGAGC 2251 CTGCAGGCCC AGCTGGACAA AGCTCTGCAG AAGGAGAAGC ACTATCTCCA 2301 GACTACCATC ACCAAAGAAG CCTATGATGC ATTATCCCGG AAGTCAGCCG 2351 CCTGCCAGGA TGACCTGACA CAAGCCCTCG AGAAGCTCAA TCACGTGACC 2401 TCAGAGACAA AGAGCCTGCA GCAAAGCTTG ACACAGACCC AAGAGAAGAA 2451 AGCTCAGCTG GAAGAGGAAA TCATTGCTTA TGAGGAAAGG ATGAAAAAGC 2501 TCAATACGGA ATTAAGAAAA CTGCGGGGCT TCCACCAGGA GAGTGAGCTG 2551 GAGGTGCACG CCTTTGACAA GAAGCTAGAG GAGATGAGCT GCCAGGTGCT 2601 GCAGTGGCAG AAGCAACACC AGAATGACCT CAAGATGCTG GCAGCCAAAG 2651 AGGAGCAGCT CAGGGAGTTC CAGGAGGAGA TGGCCGCCTT AAAAGAGAAC 2701 CTCCTTGAGG ACGATAAGGA GCCCTGCTGC CTGCCCCAGT GGTCTGTGCC 2751 CAAAGACACC TGTAGGCTCT ACCGAGGGAA TGATCAGATT ATGACCAACT 2801 TGGAGCAATG GGCAAAACAG CAGAAGGTCG CCAATGAGAA ACTAGGAAAC 2851 CAGCTCCGAG AGCAGGTGAA CTACATTGCC AAGCTGAGTG GCGAAAAGGA 2901 CCACCTCCAC AGTGTAATGG TCCACTTGCA GCAGGAAAAC AAGAAGCTGA 2951 AGAAGGAGAT AGAAGAGAAG AAGATGAAAG CCGAGAACAC AAGGCTATGC 3001 ACCAAAGCCC TAGGCCCGAG CAGAACGGAG TCCACACAGA GGGAGAAAGT 3051 GTGCGGCACC TTGGGCTGGA AGGGGTTGCC CCAGGATATG GGTCAAAGAA 3101 TGGACCTCAC CAAGTACATC GGGATGCCCC ACTGCCCGGG TTCCTCATAC 3151 TGCTAGAATC CACATCTAGC CCTGAGCAGC ATTTCCACGG GTGTTTCTTC 3201 AGAGGACAGT GAGTTCCCAG CCCTCCCTCT CTCTTGACCT GGATCAGCTC 3251 TTACAGGAGT ATATCACGGT CCCAGCCTAT TTTGCAAGAC ACTAACTTTT 3301 GTTGAGTTTT GTCCACTTCC TGCCATGGAG TGAGCTTTAG AACCATACTA 3351 CCATCTCCAG GCCCAAACTC TGAAATAAAG ACATGAGCAT GAGCAAAAAA 3401 AAAAA BLAST Results Entry AC004682 from database EMBLNEW: Homo sapiens Chromosome 16 BAC clone CIT987SK-A-259H10, complete sequence . Score = 1291, P = O.Oe+00, identities = 265/272 Medline entries No Medline entry Peptide information for frame 1 ORF from 133 bp to 3153 bp; peptide length: 1007 Category: similarity to known protein Prosite motifs: LEUCINE_ZIPPER (83-105) LEUCINE_ZIPPER (90-112) LEUCINE_ZIPPER (97-119) LEUCINE_ZIPPER (104-126) LEUCINE_ZIPPER (403-425) LEUCINE_ZIPPER (410-432) LEUCINE ZIPPER (918-940) 1 MKDEAGERDR EVSSLNSKLL SLQLDIKNLH DVCKRQRKTL QDNQLCMEEA 51 MNSSHDKKQA QALAFEESEV EFGΞSKQCHL RQLQQLKKKL LVLQQELEFH 101 TEELQTSYYS LRQYQSILEK QTSDLVLLHH HCKLKEDEVI LYEEEMGNHN 151 ENTGEKLHLA QEQLALAGDK IASLERSLNL YRDKYQSSLS NIELLECQVK 201 MLQGELGGIM GQEPENKGDH SKVRIYTSPC MIQEHQETQK RLSEVWQKVS 251 QQDDLIQELR NKLACSNALV LEREKALIKL QADFASCTAT HRYPPSSSEE 301 CEDIKKILKH LQEQKDSQCL HVEEYQNLVK DLRVELEAVS EQKRNIMKDM 351 MKLELDLHGL REETSAHIER KDKDITILQC RLQELQLEFT ETQKLTLKKD 401 KFLQEKDEML QELEKKLTQV QNSLLKKEKE LEKQQCMATE LEMTVKEAKQ 451 DKSKEAECKA LQAEVQKLKN SLEEAKQQER LAAQQAAQCK EEAALAGCHL 501 EDTQRKLQKG LLLDKQKADT IQELQRELQM LQKESSMAEK EQTΞNRKRVE 551 ELSLELSEAL RKLENSDKEK RQLQKTVAEQ DMKMNDMLDR IKHQHREQGS 601 IKCKLEEDLQ EATKLLEDKR EQLKKSKEHE KLMEGELEAL RQEFKKKDKT 651 LKENSRKLEE ENENLRAELQ CCSTQLESSL NKYNTSQQVI QDLNKEIALQ 701 KESLMSLQAQ LDKALQKEKH YLQTTITKEA YDALSRKSAA CQDDLTQALE 751 KLNHVTSETK SLQQSLTQTQ EKKAQLEEEI IAYEERMKKL NTELRKLRGF 801 HQESELEVHA FDKKLEEMSC QVLQWQKQHQ NDLKMLAAKE EQLREFQEEM 851 AALKENLLED DKEPCCLPQW SVPKDTCRLY RGNDQIMTNL EQWAKQQKVA 901 NEKLGNQLRE QVNYIAKLSG EKDHLHSVMV HLQQENKKLK KEIEEKKMKA 951 ENTRLCTKAL GPSRTESTQR EKVCGTLGWK GLPQDMGQRM DLTKYIGMPH 1001 CPGSSYC BLASTP hits Entry HS417401_1 from database TREMBL: product: "trans-Golgi p230"; Human trans-Golgi p230 mRNA, complete eds . Score = 411, P = 3.9e-34, identities = 212/862, positives = 420/862 Entry SCINTANA_1 from database TREMBL: Saccharomyces cerevisiae integrin analogue gene, complete eds. Score = 404, P = 6.2e-34, identities = 199/897, positives = 423/897 Entry HS6802_2 from database TREMBL: gene: "MYH9"; product: "dJ6802.2"; Homo sapiens DNA sequence from PAC 6802 on chromosome 22. Contains apolipoprotein L, myosin heavy chain, ESTs, CA repeat, STS and GSS. Score = 404, P = 1.9e-33, identities = 231/1028, positives = 469/1028 Entry AF092090_1 from database TREMBL: product: "cpl51"; Rattus norvegicus cpl51 mRNA, partial eds. Score = 2523, P = 3.0e-262, identities = 506/733, positives = 611/733 Alert BLASTP hits for DKFZphtes3_lgl3, frame 1 TREMBL :HSGOLGIN_l product: "256 kD golgin"; H. sapiens mRNA for golgm, N = 1, Score = 411, P = 4.4e-34 TREMBL:HS417401_1 product: "trans-Golgi p230"; Human trans-Golgi p230 mRNA, complete eds., N = 1, Score = 411, P = 4.5e-34 TREMBL :SCINTANA_1 Saccharomyces cerevisiae integrin analogue gene, complete eds., N = 1, Score = 404, p = 7. le-34 >TREMBL:HSGOLGIN_l product: "256 kD golgm"; H. sapiens mRNA for golgin Length = 2,185 HSPs: Score = 411 (61.7 bits), Expect = 4.4e-34, P = 4.4e-34 Identities = 212/816 (25%), Positives = 420/816 (51%) Query: 145 EMGNHNEN-TGEKLHLAQEQLALAGDKIASLERSLNLYRDKYQSSLSNIELLECQVKMLQ 203 +M + E+ G L +EQL ++ +ERSL+ YR KY ++ ++L+ + K LQ Sbjct: 119 DMDSEAEDLVGNSDSLNKEQLI QRLRRMERSLSSYRGKYSELVTAYQMLQREKKKLQ 175 Query: 204 GELGGIMGQEPENKGDHSKVRIYTSPCMIQEHQETQKRLSEVWQ-KVSQQDDLIQELRNK 262 G 1+ Q D S Rl +Q Q+ +K L E + + ++D I L+ + Sbjct: 176 G ILSQSQ DKSLRRIAELREELQMDQQAKKHLQEEFDASLEEKDQYISVLQTQ 227 Query: 263 LAC SNALVLEREKALIKLQADFASCTATHRYPPSSSEEC-ED—IKKILKHLQE 313 ++ + + ++ K L +L+ A P S E ED K L+ LQ+ Sbjct: 228 VSLLKQRLRNGPMNVDVLKPLPQLEPQ-AEVFTKEENPESDGEPVVEDGTSVKTLETLQQ 286 Query: 314 QKDSQ CLH-VEEYQNLVKDLRVELEAVSEQKRNIMKDMMKLELDLHGLREETSA 366 + Q C ++ ++ L E EA+ EQ ++++ K++ DLH + E+T Sbjct: 287 RVKRQENLLKRCKETIQSHKEQCTLLTSEKEALQEQLDERLQELEKIK-DLH-MAEKTKL 344 Query: 367 HIERKDKDITILQCRLQELQLEFTETQKLTLKKDKFLQEKDEMLQELEKKLTQV—QNSL 424 + +D I Q Q+ + ET++ + + L+ K+E + +L ++ Q+ Q Sbjct: 345 ITQLRDAKNLIEQLE-QDKGMVIAETKR QMHETLEMKEEEIAQLRSRIKQMTTQGEE 400 Query: 425 LKKEKELEKQQCMATELEMTVKEAKQDKSKEAECKALQAEVQKLKNSLEEAKQQERLAAQ 484 L+++KE + ++ ELE + A+ K++EA K L+AE+ + ++E+ ++ER++ Q Sbjct: 401 LREQKE-KSERAAFEELEKALSTAQ—KTEEARRK-LKAEMDEQIKTIEKTSEEERISLQ 456 Query: 485 QA-AQCKEEAA-LAGCHLEDTQRKLQKGLLLDKQKADTIQELQRELQMLQKESSMAEKEQ 542 Q ++ K+E + E+ KLQK L +K+ A QEL ++LQ ++E E+ + Sbjct: 457 QELSRVKQEVVDVMKKSSEEQIAKLQK--LHEKELARKEQELTKKLQTRERE--FQEQMK 512 Query: 543 TSNRKRVEELSLELSEALRKLENSDKEKRQLQKT—VAEQDMKMNDMLDRIKHQHREQGS 600 + K E L++S+ + E+ E+ +LQK + E + K+ D+ + Sbjct: 513 VALEKSQSEY-LKISQEKEQQESLALEELELQKKAILTESENKLRDLQQEAETYRTRILE 571 Query: 601 IKCKLEEDLQEATKLLED KREQLKKSKEHEKLMEG ELEALR-QEFKKKDKTL 651 ++ LE+ LQE +D + E+ K +KE ++E ELE+L+ Q+ + L Sbjct: 572 LESSLEKSLQENKNQSKDLAVHLEAEKNKHNKEITVMVEKHKTELESLKHQQDALWTEKL 631 Query: 652 KENSRKLEEENENLRAELQCCSTQLESSL-NKYNTSQQVIQDLNKE IALQKESLMS 706 + ++ + E E LR + C + E+ L +K Q I+++N++ + +++ L S Sbjct: 632 QVLKQQYQTEMEKLREK CEQEKETLLKDKEIIFQAHIEEMNEKTLEKLDVKQTELES 688 Query: 707 LQAQLDKALQKEKHYLQT—TITKEAYDALSRKSAACQDDLTQALEKLNHVTSETKSLQQ 764 L ++L + L K +H L+ ++ K+ D + ++ A D+ Q V S K + Sbjct: 689 LSSELSEVL-KARHKLEEELSVLKDQTDKMKQELEAKMDE—QKNHHQQQVDSIIKEHEV 745 Query: 765 SLTQTQEKKAQLEEEIIAYEERMKKLNTELRKLRGFHQESELEVHAFDKKLEEMSCQVLQ 824 S+ +T+ KA L+++I E +K+ + L++ + + E ++ + +L++ S ++ Sbjct: 746 SIQRTE--KA-LKDQINQLELLLKERDKHLKEHQAHVENLEADIKRSEGELQQASAKLDV 802 Query: 825 WQKQHQNDLKMLAAKEEQLREFQEEMAALKENLLEDDKEPCCLPQW SVPKDTC-R 878 +Q +Q+ A EQ + ++E++A L++ LL+ + E L + + KD C Sbjct: 803 FQS-YQS ATHEQTKAYEEQLAQLQQKLLDLETERILLTKQVAEVEAQKKDVCTE 855 Query: 879 LYRGNDQIMTNLEQWAKQQKVANEKLGNQLREQVNYIAKLS-GEKDHLHSVMVHLQQENK 937 L Q+ ++Q KQ +K+ + QV Y +KL G K+ + + +++EN Sbjct: 856 LDAHKIQVQDLMQQLEKQNSEMEQKVKSLT—QV-YESKLEDGNKEQEQTKQILVEKENM 912 Query: 938 KLK-KEIEEKKMKAENTRLCTK 958 L+ +E ++K+++ +L K Sbjct: 913 ILQMREGQKKEIEILTQKLSAK 934 Score = 338 (50.7 bits), Expect = 3. le-26, P = 3. le-26 Identities = 216/953 (22%), Positives = 468/953 (49%) Query: 2 KDEAGERDRE—VSSLNS-KLL-SLQLDIKNLHDVCKRQRKTLQDN-QLCM EEAM 51 K+E E D E V Ξ K L +LQ +K ++ KR ++T+Q + + C +EA+ Sbjct: 260 KEENPEΞDGEPVVEDGTSVKTLETLQQRVKRQENLLKRCKETIQSHKEQCTLLTSEKEAL 319 Query: 52 NSSHDKKQAQALAFEESEVEFGSSKQCHLRQ LQQLK—KKLLVLQQELEFHTEELQ 105 D++ + ++ + + LR ++QL+ K +++ + + + H E L+ Sbjct: 320 QEQLDERLQELEKIKDLHMAEKTKLITQLRDAKNLIEQLEQDKGMVIAETKRQMH-ETLE 378 Query: 106 TSYYSLRQYQSILEKQTSDLVLLHHHCKLKEDEVILYEEEMGNHNENTGEKLHLAQEQL- 164 + Q +S +++ T+ L K K + E E +T +K A+ +L Sbjct: 379 MKEEEIAQLRSRIKQMTTQGEELREQ-KEKSERAAFEELEKAL STAQKTEEARRKLK 434 Query: 165 ALAGDKIASLERSLNLYRDKYQSSLSNI—ELLECQVKMLQGELGGIMGQEPENKGDHSK 222 A ++I ++E++ R Q LS + E+++ K + ++ + Q+ K K Sbjct: 435 AEMDEQIKTIEKTSEEERISLQQELSRVKQEVVDVMKKSSEEQIAKL--QKLHEKELARK 492 Query: 223 VRIYTSPCMIQEHQETQKRLSEVWQKVSQQDDLIQELRNKLACSNALVLEREKALIKLQA 282 + T +E +E Q+++ +K SQ + L ++ + +L LE ++LQ Sbjct: 493 EQELTKKLQTRE-REFQEQMKVALEK-SQΞEYL—KISQEKEQQESLALEE LELQK 544 Query: 283 DFASCTATHRYPPSΞSEECEDIKKILKHLQEQKDSQCLHVEEYQNLVKDLRVELEAV-SE 341 A T + +E E + + L+ + ++E +N KDL V LEA ++ Sbjct: 545 K-AILTEΞENKLRDLQQEAETYRTRILELESSLEKS LQENKNQSKDLAVHLEAEKNK 600 Query: 342 QKRNIMKDMMKLELDLHGLREETSAHIERKDKDITI-LQCRLQELQLEFTETQKLTLKKD 400 + 1 + K + +L L+ + A K + + Q +++L+ E E +K TL KD Sbjct: 601 HNKEITVMVEKHKTELESLKHQQDALWTEKLQVLKQQYQTEMEKLR-EKCEQEKETLLKD 659 Query: 401 K FLQEKDEM-LQELEKKLTQVQNSLLKKEKELEKQQCMATELEMTVKEAKQDKS 453 K ++E +E L++L+ K T+++ SL + E+ K + E E++V + + DK Sbjct: 660 KEIIFQAHIEEMNEKTLEKLDVKQTELE-SLSSELSEVLKARHKLEE-ELSVLKDQTDKM 717 Query: 454 K-EAECKALQAEVQKLKNSLEEAKQQERLAAQQAAQC-KEEAALAGCHLEDTQRKLQKGL 511 K E E K + + + ++ ++ ++ Q+ + K++ L++ + L++ Sbjct: 718 KQELEAK-MDEQKNHHQQQVDSIIKEHEVSIQRTEKALKDQINQLELLLKERDKHLKEHQ 776 Query: 512 L-LDKQKADTIQELQRELQMLQKESSMAEKEQTSNRKRVEELSLELSEALRKLENSDKEK 570 ++ +AD 1+ + ELQ + + + Q++ ++ + +L++ +KL + + E+ Sbjct: 777 AHVENLEAD-IKRSEGELQQASAKLDVFQΞYQSATHEQTKAYEEQLAQLQQKLLDLETER 835 Query: 571 RQLQKTVAEQDMKMNDM LD--RIKHQHREQGSIK—CKLEEDLQEATKLLEDKREQL 623 L K VAE + + D+ LD +1+ Q Q K ++E+ ++ T++ E K E Sbjct: 836 ILLTKQVAEVEAQKKDVCTELDAHKIQVQDLMQQLEKQNSEMEQKVKΞLTQVYESKLEDG 895 Query: 624 KKSKEHEK—LMEGELEALRQEFKKKDKTLKENSRKLEEENENLRAELQCCSTQLESSLN 681 K +E K L+E E L+ +K K ++ ++KL + +++ + T+ ++ Sbjct: 896 NKEQEQTKQILVEKENMILQMREGQK-KEIEILTQKLSAKEDSIHILNEEYETKFKNQEK 954 Query: 682 KYNTSQQVIQDLNKEIALQKESLMSLQAQLDKALQKEKHYLQTTITKEAYDALSRKSAAC 741 K +Q +++ + + K+ L+ +A+L K L E L+ + ++ ++A + A Sbjct: 955 KMEKVKQKAKEMQETL KKKLLDQEAKLKKEL--ENTALELSQKEKQFNAKMLEMAQA 1009 Query: 742 QD-DLTQALEKLNHVTSETKSLQQSLTQTQEKKAQLEEEIIAYEERMKKLNTELRKLRGF 800 ++ A+ +L T++ + ++ SLT+ + +L + I +E KKLN + +L+ Sbjct: 1010 NΞAGISDAVSRLE—TNQKEQIE-SLTEVHRR—ELNDVISIWE KKLNQQAEELQEI 1061 Query: 801 HQESELEVHAFDKKLEEMSCQVLQW—QKQHQNDLKMLAAKEEQLREFQEEMAALKENLL 858 H E+++ ++++ E+ ++L + +K+ N ++ KEE +++ + L+E L Sb j ct: 1062 H EIQLQEKEQEVAELKQKILLFGCEKEEMNK-EITWLKEEGVKQ-DTTLNELQEQLK 1116 Query: 859 EDDKEPCCLPQWSVPKDTCRLYRGNDQIMTNLEQ—WAKQQKVANEKLGNQLREQVNYI- 915 + L Q K L + + +L++ + ++Q V + L + + +V+ + Sbjct: 1117 QKSAHVNSLAQ-DETKLKAHLEKLEVDLNKSLKENTFLQEQLVELKMLAEEDKRKVSELT 1175 Query: 916 AKLSGEKDHLHSVMVHLQQENKKLK-KEIEEKKMKAE 951 +KL + S+ ++ NK L+ K +E KK+ E Sbjct: 1176 SKLKTTDEEFQSLKSSHEKSNKSLEDKSLEFKKLSEE 1212 Score = 337 (50.6 bits), Expect = 4.0e-26, P = 4.0e-26 Identities = 215/951 (22%), Positives = 433/951 (45%) Query: 10 REVSSLNSKLLSLQLDIKNLHDVCKRQRKTLQDNQLCMEEAMNSSHDKKQAQALAFEESE 69 +E + +++L L+ ++ K Q K L + EA + H+K+ + E+ + Sbjct: 560 QEAETYRTRILELESSLEKSLQENKNQSKDLAVHL EAEKNKHNKEIT—VMVEKHK 613 Query: 70 VEFGSSKQCHLRQLQQLKKKLLVLQQELEFHTEELQTSYYSLRQYQSILEKQTSDLVLLH 129 E S K H +Q +KL VL+Q+ + E+L+ Q + L K +++ Sbjct: 614 TELESLK—H-QQDALWTEKLQVLKQQYQTEMEKLREK CEQEKETLLKD-KEIIFQA 666 Query: 130 HHCKLKE DEVILYEEEMGNHNENTGEKL HLAQEQLALAGDKIASLERSLNLYRD 183 H ++ E +++ + + E+ + + E L H +E+L++ D+ +++ L D Sbjct: 667 HIEEMNEKTLEKLDVKQTELESLSSELSEVLKARHKLEEELSVLKDQTDKMKQELEAKMD 726 Query: 184 K YQSSLSNIELLECQVKMLQGE—LGGIMGQEPENKGDHSKVRIYTSPCMIQEHQE 237 + +Q + +I + E +V + + E L + Q + K + ++ + Sbjct: 727 EQKNHHQQQVDSI-IKEHEVSIQRTEKALKDQINQLELLLKERDK-HLKEHQAHVENLEA 784 Query: 238 TQKRLΞEVWQKVSQQDDLIQELRNKLACSNALVLEREKALIKLQADFASCTATHRYPPSS 297 KR Q+ S + D+ Q ++ ++ E+ L +LQ T R Sbjct: 785 DIKRSEGELQQASAKLDVFQSYQS ATHEQTKAYEEQLAQLQQKLLDLE-TERIL 837 Query: 298 SEECEDIKKILKHLQEQKDSQCLHVEEYQNLVKDLRVELEAVSEQKRNIMKDMMKL-ELD 356 + K + ++ QK C ++ ++ V+DL +LE + + +K + ++ E Sbjct: 838 LTKQVAEVEAQKKDVCTELDAHKIQVQDLMQQLEKQNSEMEQKVKSLTQVYESK 891 Query: 357 LH-GLREETSAHIERKDKDITILQCRL-QELQLEFTETQKLTLKKDKF—LQEKDEM-LQ 411 L G +E+ +K+ ILQ R Q+ ++E TQKL+ K+D L E+ E + Sbjct: 892 LEDGNKEQEQTKQILVEKENMILQMREGQKKEIEIL-TQKLSAKEDSIHILNEEYETKFK 950 Query: 412 ELEKKLTQVQNSLLK KEKELEKQQCMATELEMTVKEAKQDKSKEAECKALQAEVQ 466 EKK+ +V+ + K+K L+++ + ELE T E Q K K+ K L+ Q Sbjct: 951 NQEKKMEKVKQKAKEMQETLKKKLLDQEAKLKKELENTALELSQ-KEKQFNAKMLEM-AQ 1008 Query: 467 KLKNSLEEAKQQERLAAQQAAQCKEEAALAGCHLEDTQRKLQKGLLLDKQKADTIQELQR 526 + +A RL Q Q + + L D +K L Q+A+ +QE+ Sbjct: 1009 ANSAGISDAVS—RLETNQKEQIESLTEVHRRELNDVISIWEKKL NQQAEELQEIH- 1062 Query: 527 ELQMLQKESSMAEKEQT SNRKRV EELSLELSEALRKLENSDKEKRQLQ 574 E+Q+ +KE +AE +Q K + +E ++ L +L+ K+K Sbjct: 1063 EIQLQEKEQEVAELKQKILLFGCEKEEMNKEITWLKEEGVKQDTTLNELQEQLKQKSAHV 1122 Query: 575 KTVAEQDMKMNDMLDRIKHQHREQGSIKCKLEEDLQEATKLLEDKREQLKKSKEHEKLME 634 ++A+ + K+ L++++ + L+E L E L E+ + ++ + K + Sbjct: 1123 NSLAQDETKLKAHLEKLEVDLNKSLKENTFLQEQLVELKMLAEEDKRKVSELTSKLKTTD 1182 Query: 635 GELEALRQEFKKKDKTLKENSRKLEEENENLRAELQCCSTQLESSLNKYNTSQQVIQDLN 694 E ++L+ +K +K+L++ S + ++ +E L +L C + E+ L T++ + + Sbjct: 1183 EEFQSLKSSHEKSNKSLEDKSLEFKKLSEELAIQLDICCKKTEALLEA-KTNELINISSS 1241 Query: 695 KEIALQKESLMSLQAQLDKALQKEKHYLQTTITKEAYDALSRKSAACQDDLT QALE 750 K A+ + Q + K KE ++T E +A R+ Q+ L QA Sbjct: 1242 KTNAILSR-ISHCQHRTTKV—KEALLIKTCTVSEL-EAQLRQLTEEQNTLNISFQQATH 1297 Query: 751 KLNHVTSETKSLQQSLTQTQEKKAQLEEEIIAYEERMKKLN TELRK—LRGFHQESE 805 +L ++ KS++ + +K L++E ++ + T+L+K + + Sbjct: 1298 QLEEKENQIKSMKADIESLVTEKEALQKEGGNQQQAASEKESCITQLKKELSENINAVTL 1357 Query: 806 LEVHAFDKKLE—EMΞCQVLQWQKQHQNDLKMLAAKEEQLREFQEEMAALKENLLEDDKE 863 ++ +KK+E +S Q+ Q QN + L+ KE + +++ K LL D + Sbjct: 1358 MKEELKEKKVEISSLSKQLTDLNVQLQNSIS-LSEKEAAISΞLRKQYDEEKCELL-DQVQ 1415 Query: 864 PCCLPQWSVPKDTCRLYRGNDQIMTNLEQWAKQQKVANEKLGNQLRE QVNYIAKLSG 920 ++ K+ D +W K+ + + N ++E Q+ +K + Sbjct: 1416 DLSFKVDTLΞKEKISALEQVDDWSNKFSEWKKKAQSRFTQHQNTVKELQIQLELKSKEAY 1475 Query: 921 EKDH-LHSVMVHLQQENKK LKKEIEEKKMKAE 951 EKD ++ + L Q+NK+ LK E+E+ K K E Sbjct: 1476 EKDEQINLLKEELDQQNKRFDCLKGEMEDDKSKME 1510 Score = 332 (49.8 bits), Expect = 1.4e-25, P = 1.4e-25 Identities = 209/953 (21%), Positives = 438/953 (45%) Query: 1 MKDEAGERDREVSSLNSKLLSLQLDIKNLHDVCKRQRKTLQDNQLCMEEAMNS SHD 56 MK + E+ ++ L+ K L+ + + + + R+R+ + ++ +E++ + S + Sbjct: 470 MKKSSEEQIAKLQKLHEKELARK-EQELTKKLQTREREFQEQMKVALEKSQSEYLKISQE 528 Query: 57 KKQAQALAFEESEVEFGSSKQCHLRQLQQLKKKLLVLQQELEFHTEELQTSYYSLRQYQS 116 K+Q ++LA EE E++ K+ L + + KL LQQE E + + SL + Sbjct: 529 KEQQESLALEELELQ KKAILTESEN KLRDLQQEAETYRTRILELESSLEKSLQ 581 Query: 117 ILEKQTSDLVLLHHHCKLKEDE—VILYEE EMGNHNENT—GEKLHLAQEQLALA 167 + Q+ DL + K K ++ ++ E+ E H ++ EKL + ++Q Sbjct: 582 ENKNQSKDLAVHLEAEKNKHNKEITVMVEKHKTELESLKHQQDALWTEKLQVLKQQYQTE 641 Query: 168 GDKIASL—ERSLNLYRDK YQSSLS—NIELLECQVKMLQGELGGIMGQEPENKGDH 220 +K+ + L +DK +Q+ + N + LE ++ + Q EL + + E Sbjct: 642 MEKLREKCEQEKETLLKDKEIIFQAHIEEMNEKTLE-KLDVKQTELESLSSELSEVLKAR 700 Query: 221 SKVRIYTSPCMIQEHQETQKRLSEVWQKVSQQDDLIQELRNKLACSNALVLEREKALIKL 280 K+ S ++++ +T K E+ K+ +Q + Q+ + + + + ++R + +K Sbjct: 701 HKLEEELS—VLKD--QTDKMKQELEAKMDEQKNHHQQQVDSIIKEHEVΞIQRTEKALKD 756 Query: 281 QADFASCTATHR—YPPSSSEECEDIKKILKHLQEQKDSQCLHVEEYQNLVKDLRVELEA 338 Q + R + E+++ +K + + ++ +Q+ + +A Sbjct: 757 QINQLELLLKERDKHLKEHQAHVENLEADIKRSEGELQQASAKLDVFQSYQSATHEQTKA 816 Query: 339 VSEQKRNIMKDMMKLELDLHGLREETSAHIERKDKDITILQCRLQELQLEFTETQKLTLK 398 EQ + + ++ LE + L ++ A +E + KD+ C EL + Q L + Sbjct: 817 YEEQLAQLQQKLLDLETERILLTKQV-AEVEAQKKDV CT—ELDAHKIQVQDLMQQ 869 Query: 399 KDKFLQEKDEMLQELEKKLTQVQNΞLLKK-EKELEKQQCMATELEMTVKEAKQDKSKEAE 457 +K + EM Q++ K LTQV S L+ KE E+ + + E E + + ++ + KE E Sbjct: 870 LEK QNSEMEQKV-KSLTQVYESKLEDGNKEQEQTKQILVEKENMILQMREGQKKEIE 925 Query: 458 C—KALQAEVQKLKNSLEEAKQQERLAAQQAAQCKEEAALAGCHLEDTQRK—LQKGLLL 513 + L A+ + EE + + + ++ + K++A +++T +K L + L Sbjct: 926 ILTQKLSAKEDΞIHILNEEYETKFKNQEKKMEKVKQKAK EMQETLKKKLLDQEAKL 981 Query: 514 DKQKADTIQEL-QRELQMLQKESSMAEKEQTSNRKRVEELSLELSEALRKLENSDKEKRQ 572 K+ +T EL Q+E Q K MA+ V L E + L ++ +R+ Sbjct: 982 KKELENTALELSQKEKQFNAKMLEMAQANSAGISDAVSRLETNQKEQIESL—TEVHRRE 1039 Query: 573 LQKTVAEQDMKMNDMLDRIKHQHREQGSIKCKLEEDLQEATKLLEDKREQLKKS KE 628 L ++ + K+N + ++ H Q K + +L++ L ++E++ K KE Sbjct: 1040 LNDVISIWEKKLNQQAEELQEIHEIQLQEKEQEVAELKQKILLFGCEKEEMNKEITWLKE 1099 Query: 629 HEKLMEGELEALRQEFKKKDKTLKENSRKLEEENENLRAELQCCSTQLESSLNKYNTSQQ 688 + L L+++ K+K + NS L ++ L+A L+ L SL + Q+ Sbjct: 1100 EGVKQDTTLNELQEQLKQKSAHV--NS--LAQDETKLKAHLEKLEVDLNKSLKENTFLQE 1155 Query: 689 VIQDLNKEIALQKESLMSLQAQL DKALQ—KEKHYLQTTITKEA YDALSRKSAA 740 + +L K + L ++L D+ Q K H ++ + LS + A Sbjct: 1156 QLVELKMLAEEDKRKVSELTSKLKTTDEEFQSLKSSHEKSNKSLEDKSLEFKKLSEE-LA 1214 Query: 741 CQDDL TQAL EKLNHVTSETKSLQQSLTQTQEKKAQLEEEIIAYEERMKKL 790 Q D+ T+AL E +N +S+T ++ ++ Q + +++E ++ + +L Sbjct: 1215 IQLDICCKKTEALLEAKTNELINISSSKTNAILSRISHCQHRTTKVKEALLIKTCTVSEL 1274 Query: 791 NTELRKLRGFHQESELEVHAFDKKLEEMSCQVLQWQKQHQNDLKMLAAKEEQLREFQEEM 850 +LR+L + +LEE Q+ K + D++ L ++E L Q+E Sbjct: 1275 EAQLRQLTEEQNTLNISFQQATHQLEEKENQI KSMKADIESLVTEKEAL QKEG 1327 Query: 851 AALKENLLEDDKEPCCLPQWSVPKDTCRLYRGNDQIMTNLEQWAKQQKVANEKLGNQLRE 910 + +KE C + Q + K+ N +T +++ K++KV L QL + Sbjct: 1328 G—NQQQAASEKESC-ITQ—LKKELSE NINAVTLMKEELKEKKVEISSLSKQLTD 1378 Query: 911 QVNYIAKLSGEKDHLHSVMVHLQQENKKLKKEIEEKKMKAE 951 Q+ LS ++ + S+ +E +L ++++ K + Sbjct: 1379 LNVQLQNSISLSEKEAAISSLRKQYDEEKCELLDQVQDLSFKVD 1422 Score = 329 (49.4 bits), Expect = 2.9e-25, P = 2.9e-25 Identities = 226/941 (24%), Positives = 444/941 (47%) Query: 61 QALAFEESEVE—FGSSKQCHLRQLQQLKKKLLVLQQELEFHTEELQTSYYSLRQYQSIL 118 Q L E+ +++ S+ LR++ +L+++L + QQ + EE S QY S+L Sbjct: 165 QMLQREKKKLQGILSQSQDKSLRRIAELREELQMDQQAKKHLQEEFDASLEEKDQYISVL 224 Query: 119 EKQTSDLVLLHHHCKLKEDEV ILYEEEMGNHNENT GEKL HLAQEQLALA 167 + Q S L + + D + + + E+ EN GE + + + L Sbjct: 225 QTQVSLLKQRLRNGPMNVDVLKPLPQLEPQAEVFTKEENPESDGEPVVEDGTSVKTLETL 284 Query: 168 GDKIASLERSLNLYRDKYQSSLSNIELLECQVKMLQGELGGIMGQEPENKGDHSKVRIYT 227 ++ E L ++ QS LL + + LQ +L + QE E D + + Sbjct: 285 QQRVKRQENLLKRCKETIQSHKEQCTLLTSEKEALQEQLDERL-QELEKIKD LHMAE 340 Query: 228 SPCMIQEHQETQKRLSEVWQKVSQQDDLIQELRNKLACSNALVLEREKALIKLQADFASC 287 +1 + ++ + + ++ Q +1 E + ++ L ++ E+ + +L++ Sbjct: 341 KTKLITQLRDAKNLIEQLEQDKGM VIAETKRQM--HETLEMKEEE-IAQLRSRIKQM 394 Query: 288 TATH RYPPSSSEEC—EDIKKILKHLQEQKDSQCLHVEEYQNLVKDL RVE 335 T R SE E+++K L Q+ ++++ E +K + R+ Sbjct: 395 TTQGEELREQKEKSERAAFEELEKALSTAQKTEEARRKLKAEMDEQIKTIEKTSEEERIS 454 Query: 336 LEA-VSEQKRNIMKDMMKL—ELDLHGLREETSAHIERKDKDITILQCRLQELQLEFTET 392 L+ +S K+ ++ D+MK E + L++ + RK++++T +LQ + EF E Sbjct: 455 LQQELSRVKQEVV-DVMKKSSEEQIAKLQKLHEKELARKEQELTK KLQTREREFQEQ 510 Query: 393 QKLTLKKDKFLQEKDEMLQELEKKLTQVQNSLLKKEKELEKQQCMATELEMTVKEAKQDK 452 K+ L+K + E ++ QE E+ Q SL +E EL+K+ + TE E +++ +Q+ Sbjct: 511 MKVALEKSQ—SEYLKISQEKEQ QESLALEELELQKKAIL-TESENKLRDLQQE- 561 Query: 453 SKEAECKALQAEVQKLKNSLEEAKQQER LAAQQAAQCKEEAALAGCHLEDTQR-K 506 ++ + L+ E L+ SL+E K Q + L A++ KE + H + + K Sbjct: 562 AETYRTRILELE-SSLEKSLQENKNQSKDLAVHLEAEKNKHNKEITVMVEKHKTELESLK 620 Query: 507 LQKGLLLDKQKADTIQELQRELQMLQKESSMAEKEQTSNRKRVEELSLELSEALRK-LEN 565 Q+ L ++ Q+ Q E++ L +E EKE K + + E K LE Sbjct: 621 HQQDALWTEKLQVLKQQYQTEMEKL-REKCEQEKETLLKDKEII-FQAHIEEMNEKTLEK 678 Query: 566 SDKEKRQLQKTVAEQDMKMNDMLDRIKHQHREQGSI-KCKLEEDLQEA-TKLLEDKR—E 621 D ++ +L+ +E ++++L + +H+ E+ S+ K + ++ QE K+ E K + Sbjct: 679 LDVKQTELESLSSE LSEVL-KARHKLEEELSVLKDQTDKMKQELEAKMDEQKNHHQ 733 Query: 622 QLKKS—KEHEKLMEGELEALRQEFKKKDKTLKENSRKLEEEN ENLRAELQCCSTQL 676 Q S KEHE ++ +AL+ + + + LKE + L+E ENL A+++ +L Sbjct: 734 QQVDSIIKEHEVSIQRTEKALKDQINQLELLLKERDKHLKEHQAHVENLEADIKRSEGEL 793 Query: 677 ESSLNKYNTSQQVIQDLNKEIALQKESLMSLQAQLDKALQKEKHYLQTTITKEAYDALSR 736 + + K + Q +++ +E L LQ +L L+ E+ L TK+ + ++ Sbjct: 794 QQASAKLDVFQSYQSATHEQTKAYEEQLAQLQQKL-LDLETERILL TKQVAEVEAQ 848 Query: 737 KSAACQD DLTQALEKLNHVTSETKSLQQSLTQTQEKKAQ—LEEEIIAYEE 785 K C + DL Q LEK N SE + +SLTQ E K + +E+ + Sbjct: 849 KKDVCTELDAHKIQVQDLMQQLEKQN SEMEQKVKSLTQVYESKLEDGNKEQEQTKQI 905 Query: 786 RMKKLNTELRKLRGFHQESELEVHAFDKKLEEMSCQVL—QWQKQHQNDLKMLAAKEEQL 843 ++K N L+ G Q+ E+E+ +E S +L +++ + +N K + +++ Sbjct: 906 LVEKENMILQMREG—QKKEIEILTQKLSAKEDSIHILNEEYETKFKNQEKKMEKVKQKA 963 Query: 844 REFQEEMAALKENLLEDDKEPCCLPQWSVPKDTCRLYRGNDQIMTNLEQWAKQQKV 899 +E QE LK+ LL+ + + L + + L + Q + + A+ Sbjct: 964 KEMQE TLKKKLLDQEAK LKK-ELENTALELSQKEKQFNAKMLEMAQANSAGIΞD 1016 Query: 900 ANEKLGNQLREQVNYIAKLSG-EKDHLHSVMVH-LQQENKKLKK—EIEEKKMKAENTRL 955 A +L +EQ+ + ++ E + + S+ L Q+ ++L++ EI+ ++ + E L Sbjct: 1017 AVSRLETNQKEQIESLTEVHRRELNDVISIWEKKLNQQAEELQEIHEIQLQEKEQEVAEL 1076 Query: 956 CTKALGPSRTESTQREKVCGTLGWKGLPQD 985 K L E + K L +G+ QD Sbjct: 1077 KQKIL-LFGCEKEEMNKEITWLKEEGVKQD 1105 Score = 326 (48.9 bits), Expect = 6.0e-25, P = 6.0e-25 Identi ties = 220/907 (24%), Positives = 444/907 (48%) Query: 67 ESEVEFGSSKQCHLRQLQQLKKKLLVLQQELEFHTEELQTSYYSLRQYQSILE KQTS 123 E+E G+S + QL Q +++ EL T+Y L++ + L+ Q+ Sbjct: 123 EAEDLVGNΞDSLNKEQLIQRLRRMERSLSSYRGKYSELVTAYQMLQREKKKLQGILSQSQ 182 Query: 124 DLVLLHHHCKLKEDEVILYEEEMGNHNENTGEKLHLAQEQLALAGDKIASLERSLNLYRD 183 D L +L+E+ + +++ H + E+ + E+ 1+ L+ ++L + Sbjct: 183 DKSL-RRIAELREE--LQMDQQAKKHLQ EEFDASLEE KDQYISVLQTQVSLLKQ 233 Query: 184 KYQSSLSNIELLECQVKMLQGELGGIMGQE-PENKG DHSKVR-IYTSPCMIQEHQ 236 + ++ N+++L+ + L+ + +E PE+ G D + V+ + T ++ + Sbjct: 234 RLRNGPMNVDVLK-PLPQLEPQAEVFTKEENPEΞDGEPVVEDGTΞVKTLETLQQRVKRQE 292 Query: 237 ETQKRLSEVWQKVSQQDDLIQELRNKLACSNALVLEREKALIKLQADFASCTATHRYPPS 296 KR E Q +Q L+ K A L ER + L K++ D T Sbjct: 293 NLLKRCKETIQSHKEQCTLLTS—EKEALQEQLD-ERLQELEKIK-DLHMAEKTKLIT— 346 Query: 297 SSEECEDIKKILKHLQEQKDSQCLHVEEYQNLVKDLRVELEAVSEQKRNIMKDMMKLELD 356 + D K +++ L++ K + E + + + L ++ E ++ Q R+ +K M + Sbjct: 347 QLRDAKNLIEQLEQDKGM— IAETKRQMHETLEMKEEEIA-QLRSRIKQMTTQGEE 400 Query: 357 LHGLREETS-AHIERKDKDITILQCRLQE LQLEFTETQKLTLKKDKFLQEKDEMLQ 411 L +E++ A E +K ++ Q + +E L+ E E K T++K +E+ + Q Sbjct: 401 LREQKEKSERAAFEELEKALSTAQ-KTEEARRKLKAEMDEQIK-TIEKTSE-EERISLQQ 457 Query: 412 ELEKKLTQVQNSLLKK-EKELEKQQCMATELEMTVKEAKQDKSKEAECKALQAEVQKLKN 470 EL + +V + + K E+++ K Q + E E+ KE Q+ +K+ + + + + Q +K Sbjct: 458 ELSRVKQEVVDVMKKSSEEQIAKLQKLH-EKELARKE--QELTKKLQTREREFQEQ-MKV 513 Query: 471 SLEEAKQQERLAAQQAAQCKEEAALAGCHLEDTQRKLQ-KGLLLD-KQKADTIQELQREL 528 +LE++ Q E L Q + +E AL L+ + + L D +Q+A+T + EL Sbjct: 514 ALEKS-QSEYLKISQEKEQQESLALEELELQKKAILTESENKLRDLQQEAETYRTRILEL 572 Query: 529 QMLQKESSMAEKEQTSNRKRVEELSLELSEALRKLENS-DKEKRQLQKTVAEQDMKMNDM 587 + E S+ E + S V L E ++ +++ +K K +L+ +QD + Sbjct: 573 ES-SLEKSLQENKNQSKDLAVH-LEAEKNKHNKEITVMVEKHKTELESLKHQQDALWTEK 630 Query: 588 LDRIKHQHR-EQGSIKCKLEEDLQEATKLLEDKRE--QLKKSKEHEKLMEGELEALRQEF 644 L +K Q++ E ++ K E QE LL+DK Q + +EK +E +L+ + E Sbjct: 631 LQVLKQQYQTEMEKLREKCE QEKETLLKDKEIIFQAHIEEMNEKTLE-KLDVKQTEL 686 Query: 645 KKKDKTLKE— SR-KLEEENENLRAELQCCSTQLESSLNKY-NTSQQVIQDLNKE—IA 698 + L E +R KLEEE L+ + +LE+ +++ N QQ + + KE ++ Sbjct: 687 ESLSSELSEVLKARHKLEEELSVLKDQTDKMKQELEAKMDEQKNHHQQQVDSIIKEHEVS 746 Query: 699 LQK-ESLMSLQA-QLDKAL-QKEKHYLQTTITKEAYDALSRKΞ AACQDDLTQAL 749 +Q+ E + Q QL+ L +++KH + E +A ++S A+ + D+ Q+ Sbjct: 747 IQRTEKALKDQINQLELLLKERDKHLKEHQAHVENLEADIKRSEGELQQASAKLDVFQSY 806 Query: 750 EKLNHVTSETKSLQQSLTQTQEKKAQLEEEIIAYEERMKKLNTELRKLRGFHQEΞELEVH 809 + H +TK+ ++ L Q Q+K LE E I +++ ++ + + + +++V Sbjct: 807 QSATH—EQTKAYEEQLAQLQQKLLDLETERILLTKQVAEVEAQKKDVCTELDAHKIQVQ 864 Query: 810 AFDKKLEEMSCQVLQWQKQHQN—DLKMLAAKEEQLREFQEEMAALKENLL EDDKE 863 ++LE+ + ++ Q K + K+ +EQ E +++ KEN++ E K+ Sbjct: 865 DLMQQLEKQNSEMEQKVKSLTQVYEΞKLEDGNKEQ—EQTKQILVEKENMILQMREGQKK 922 Query: 864 PC-CLPQ-WSVPKDTCRLYRGNDQIMTNLE-QWAKQQKVANE—KLGNQLREQV-NYIAK 917 L Q S +D+ + N++ T + Q K +KV + ++ L++++ + AK Sbjct: 923 EIEILTQKLSAKEDSIHIL—NEEYETKFKNQEKKMEKVKQKAKEMQETLKKKLLDQEAK 980 Query: 918 LSGEKDHLHSVMVHLQQENKKLKKEIEEKKMKAENTRLCTKALGPSRTESTQREKV 973 L K L + + L Q+ K+ ++ E M N+ + A+ SR E+ Q+E++ Sbjct: 981 L KKELENTALELSQKEKQFNAKMLE—MAQANSAGISDAV—SRLETNQKEQI 1029 Score = 318 (47.7 bits). Expect = 4.4e-24, P = 4.4e-24 Identities = 184/827 (22%), Positives = 405/827 (48%) Query: 1 MKDEAGERDREVSSLNSKLLSLQLDIKNLHDVCKRQRKTLQDNQLCMEEAMNSSHDKK-Q 59 ++ E G + + S S + L+ ++ + ++ L++ ++ + D Q Sbjct: 1323 LQKEGGNQQQAASEKESCITQLKKELSENINAVTLMKEELKEKKVEISSLSKQLTDLNVQ 1382 Query: 60 AQ-ALAFEESEVEFGSSKQCHLRQLQQLKKKLLVLQQELEFHTEELQTSYYS-LRQYQS- 116 Q +++ E E S + +Q + K +LL Q+L F + L Ξ L Q Sbjct: 1383 LQNSISLSEKEAAISSLR KQYDEEKCELLDQVQDLSFKVDTLSKEKISALEQVDDW 1438 Query: 117 ILE-KQTSDLVLLHHHCKLKEDEVILYEEEMGNHNENTGEKLHLAQEQLALAGDKIA 172 E K+ + H +KE ++ L + + ++ E+++L +E+L + Sbjct: 1439 SNKFSEWKKKAQSRFTQHQNTVKELQIQLELKSKEAYEKD—EQINLLKEELDQQNKRFD 1496 Query: 173 SLERSLNLYRDKYQSSLSNIEL-LECQVKMLQGELGGIMGQEP-ENKGDHSKVRIYTSPC 230 L+ + + K + SN+E L+ Q + EL + Q+ E + + ++ Y Sbjct: 1497 CLKGEMEDDKSKMEKKESNLETELKSQTARIM-ELEDHITQKTIEIESLNEVLKNYNQQK 1555 Query: 231 MIQEHQETQKRLSEVWQKVSQQDDLIQELRNKLACSNALVLEREKALIKLQADFASCTAT 290 I EH+E ++L + ++D+ ++E K+ L LE + +K + + Sbjct: 1556 DI-EHKELVQKLQHFQELGEEKDNRVKEAEEKI LTLENQVYSMKAELETKKKELE 1609 Query: 291 HRYPPSSSEECEDIKKILKHLQEQKDSQCLHVE-EYQNLVKDLRVELEAVSEQKRNIMKD 349 H S+E E++K + L+ + ++ ++ + + + ++ +L + E+K ++ Sbjct: 1610 HVNLSVKΞKE-EELKALEDRLESESAAKLAELKRKAEQKIAAIKKQLLSQMEEK EE 1664 Query: 350 MMKLELDLHGLREETSAHIERKDKDITILQCRLQELQLEFTETQKL--TLKKDKFLQEKD 407 K + H E + ++ +++++ IL+ +L+ ++ +ET + + K E++ Sbjct: 1665 QYKKGTESH—LSELNTKLQEREREVHILEEKLKSVESSQSETLIVPRSAKNVAAYTEQE 1722 Query: 408 EM LQEL-EKKLTQVQNSLLKKEKEL EKQQCMATELEMTVK-EAKQDKSKE 455 E +Q+ E+K++ +Q +L +KEK L EK++ +++ EM + + + K + Sbjct: 1723 EADSQGCVQKTYEEKISVLQRNLTEKEKLLQRVGQEKEETVSSHFEMRCQYQERLIKLEH 1782 Query: 456 AECKAL—QAEVQKLKNSLEEAKQQERLAAQQAAQCK—EEAALAGCHLEDTQRKLQKGL 511 AE K Q+ + L+ LEE ++ L Q + + + A +LE+ +QK L Sbjct: 1783 AEAKQHEDQSMIGHLQEELEEKNKKYSLIVAQHVEKEGGKNNIQAKQNLENVFDDVQKTL 1842 Query: 512 LLDKQKADTIQELQRELQMLQKESSMAEKEQTSNRKRVEELS--LELSEALRKLENSDKE 569 ++K T Q L+++++ L +S + +++ +R +EEL+ E +AL++++ +K Sbjct: 1843 QEKELTCQILEQKIKEL—DSCLVRQKEV-HRVEMEELTSKYEKLQALQQMDGRNKP 1896 Query: 570 KRQLQKTVAEQD MKMNDMLDRIKHQHREQGSIKCKLEEDLQEATKLLEDKREQLKK- 625 L++ E+ + +L ++ QH + E + Q+ K + ++ L+ Sbjct: 1897 TELLEENTEEKSKSHLVQPKLLSNMEAQHNDLEFKLAGAEREKQKLGKEIVRLQKDLRML 1956 Query: 626 SKEHEKLMEGELEALRQEFKKKDKTLKENSRKLEEENENLRAELQCCSTQLESSLNKYNT 685 KEH++ ELE L++E+ + E K+++E E+L EL+ ST L+ + ++NT Sbjct: 1957 RKEHQQ ELEILKKEYDQ EREEKIKQEQEDL--ELKHNST-LKQLMREFNT 2003 Query: 686 S-QQVIQDLNKEIALQKESLMSLQAQLDKALQKEKHYLQTTITKEAYDALSRKSAACQDD 744 Q Q+L I ++A+L ++ Q+E + L I E D L R +A ++ Sbjct: 2004 QLAQKEQELEMTIKETINKAQEVEAELLESHQEETNQLLKKIA-EKDDDLKR-TAKRYEE 2061 Query: 745 LTQALEKLNHVTSETKSLQQSLTQTQEKKAQ-LEEEIIAYEERMK—KLNTELRKLRGFH 801 + A E+ +T++ + LQ L + Q+K Q LE+E + + +L T+L + Sbjct: 2062 ILDAREE—EMTAKVRDLQTQLEELQKKYQQKLEQEENPGNDNVTIMELQTQLAQKTTLI 2119 Query: 802 QESELEVHAFDKKLEEMSCQVLQWQK 827 +S+L+ F +++ + ++ +++K Sbjct: 2120 SDSKLKEQEFREQIHNLEDRLKKYEK 2145 Score = 316 (47.4 bits), Expect = 7.1e-24, P = 7.1e-24 Identities = 213/977 (21%), Positives = 454/977 (46%) Query: 4 EAGERD-REVSSLNSKLLΞLQLD-IKNLHDVCKRQRKTLQDNQLCMEEAMNSSHDKKQAQ 61 E R+ +V S+ K L+ Q + ++ +H++ + Q K + +L + + ++ + Sbjct: 1034 EVHRRELNDVISIWEKKLNQQAEELQEIHEI-QLQEKEQEVAELKQKILLFGCEKEEMNK 1092 Query: 62 ALAFEESEVEFGSSKQCHLRQLQ-QLKKKLL VLQQE--LEFHTEELQTSYYSLRQY 114 + + + E G + L +LQ QLK+K + Q E L+ H E+L+ + Sbjct: 1093 EITWLKEE GVKQDTTLNELQEQLKQKSAHVNSLAQDETKLKAHLEKLEVDLNKSLKE 1149 Query: 115 QSILEKQTΞDLVLLHHHCKLKEDEV ILYEEEMGNHNENTGEKLHLAQEQLALAGDKI 171 + L++Q +L +L K K E+ + +E +++ EK + + E +L K+ Sbjct: 1150 NTFLQEQLVELKMLAEEDKRKVSELTSKLKTTDEEFQSLKSSHEKΞNKSLEDKSLEFKKL 1209 Query: 172 AS-LERSLNLYRDKYQSSLS—NIELLECQVKMLQGELGGIMGQEPENKGDHSKVRIYTS 228 + L L++ K ++ L EL+ L I +++ K + Sbjct: 1210 SEELAIQLDICCKKTEALLEAKTNELINISSSKTNAILSRI—SHCQHRTTKVKEALLIK 1267 Query: 229 PCMIQEHQ ETQKRLSEVWQKVSQQ-DDLIQELRNKLACSNALVLEREKALIKL 280 C + E + E Q L+ +Q+ + Q ++ ++++ A +LV E+E L Sbjct: 1268 TCTVSELEAQLRQLTEEQNTLNISFQQATHQLEEKENQIKSMKADIESLVTEKEA L 1323 Query: 281 QADFASCTATHRYPPSSSEECEDIKKILKHLQEQKDSQCLHVEEYQNLVKDLRVELEAVS 340 Q + + + S E C I ++ K L E ++ L EE +K+ +VE+ ++S Sbjct: 1324 QKEGGN QQQAASEKESC—ITQLKKELSENINAVTLMKEE LKEKKVEISSLS 1373 Query: 341 EQKRNIMKDMMKLELDLHGLREETSAHIERKDKDITILQCRLQEL--QLEFTETQKLT-L 397 +Q ++ + + L S+ ++ D++ L ++Q+L +++ +K++ L Sbjct: 1374 KQLTDLNVQLQN-SISLSEKEAAISΞLRKQYDEEKCELLDQVQDLSFKVDTLSKEKISAL 1432 Query: 398 KK-DKFLQEKDEMLQELEKKLTQVQNSLLKKEKELEKQQCMATELEMTV KEAKQDKΞ 453 ++ D + + E ++ + + TQ QN++ + + +LE + A E + + KE ++ Sbjct: 1433 EQVDDWSNKFSEWKKKAQSRFTQHQNTVKELQIQLELKSKEAYEKDEQINLLKEELDQQN 1492 Query: 454 KEAECKALQAEVQKLKNSLEEAKQQERLAAQQAAQCKEEAALAGCHLE-DTQRKLQKGLL 512 K +C + E K K +E+ + L +Q A + E + +E ++ ++ K Sbjct: 1493 KRFDCLKGEMEDDKSKMEKKESNLETELKSQTARIMELEDHITQKTIEIESLNEVLKNY- 1551 Query: 513 LDKQKADTIQELQRELQMLQKESSMAEKEQTSNRKRVEELΞLELSEALRKLENSDKEKRQ 572 ++QK +EL ++LQ Q+ + +++ L ++ +LE KE Sbjct: 1552 -NQQKDIEHKELVQKLQHFQELGEEKDNRVKEAEEKILTLENQVYSMKAELETKKKELEH 1610 Query: 573 LQKTVAEQDMKMNDMLDRIKHQHREQ-GSIKCKLEEDLQEATKLL EDKREQLKKSK 627 + +V ++ ++ + DR++ + + +K K E+ + K L E+K EQ KK Sbjct: 1611 VNLSVKΞKEEELKALEDRLESESAAKLAELKRKAEQKIAAIKKQLLSQMEEKEEQYKKGT 1670 Query: 628 EHEKLMEGELEALRQEFKKKDKTLKENSRKLEE-ENENL RAELQCCSTQLESSLNK 682 E EL QE +++ L+E + +E ++E L A+ T+ E + ++ Sbjct: 1671 ESHL SELNTKLQEREREVHILEEKLKSVESSQΞETLIVPRSAKNVAAYTEQEEADSQ 1727 Query: 683 YNTSQQVIQDLNKEIALQKESLMSLQAQLDKALQKEKHYLQTTITKEAYDALSRKSA 739 T ++ I L + + +KE L+ Q +K H+ +E L A Sbjct: 1728 GCVQKTYEEKISVLQRNLT-EKEKLLQRVGQ-EKEETVSSHFEMRCQYQERLIKLEHAEA 1785 Query: 740 ACQDDLTQALEKLNHVTSET--KSLQQSLTQTQEKKAQLEEEIIAYEERMKKLNTELRKL 797 +D Q++ + H+ E K+ + SL Q + + + I ++ ++ + +++K Sbjct: 1786 KQHED—QSM—IGHLQEELEEKNKKYSLIVAQHVEKEGGKNNIQAKQNLENVFDDVQKT 1841 Query: 798 RGFHQESELEVHAFDKKLEEM-SCQVLQWQKQHQNDLKMLAAKEEQLREFQEEMAALKEN 856 QE EL ++K++E+ SC V Q ++ H+ +++ L +K E+L+ Q+ K Sbjct: 1842 L QEKELTCQILEQKIKELDSCLVRQ-KEVHRVEMEELTSKYEKLQALQQMDGRNKPT 1897 Query: 857 -LLEDDKEPCCLPQWSVPKDTCRLYRGNDQIMTNLEQWAKQQKVANEKLGNQLREQVNYI 915 LLE++ E PK + ++ + L A+++K +KLG ++ + Sbjct: 1898 ELLEENTEEKSKSHLVQPKLLSNMEAQHNDLEFKLAG-AEREK QKLGKEIVRLQKDL 1953 Query: 916 AKLΞGE-KDHLHSVMVHLQQENK-KLKKEIEEKKMKAENTRLCTKALGPSRTESTQREK 972 L E + L + QE + K+K+E E+ ++K +T + + T+ Q+E+ Sbjct: 1954 RMLRKEHQQELEILKKEYDQEREEKIKQEQEDLELKHNST—LKQLMREFNTQLAQKEQ 2010 Score = 301 (45.2 bits), Expect = 2.9e-22, P = 2.9e-22 Identities = 221/952 (23%), Positives = 441/952 (46%) Query: 1 MKDEAGERDREVSSLNSKLLSLQLDIKNLHDVCKRQRKTLQDNQL CMEEAMNSSHD- 56 +K A E R+VS L SKL + + ++L ++ K+L+D L + E + D Sbjct: 1160 LKMLAEEDKRKVSELTSKLKTTDEEFQSLKSSHEKSNKSLEDKSLEFKKLSEELAIQLDI 1219 Query: 57 —KKQAQALAFEESE-VEFGSΞK-QCHLRQLQQLKKKLLVLQQELEFHT EELQTSYY 109 KK L + +E + SSK L ++ + + +++ L T EL+ Sbjct: 1220 CCKKTEALLEAKTNELINISSSKTNAILSRISHCQHRTTKVKEALLIKTCTVSELEAQLR 1279 Query: 110 SLRQYQSILEKQTSDLVLLHHHCKLKEDEVILYEEEMGNHNENTGEKLHLAQE QLAL 166 L + Q+ L H + KE+++ + ++ EK L +E Q Sbjct: 1280 QLTEEQNTLNISFQQAT HQLEEKENQIKSMKADI ESLVTEKEALQKEGGNQQQA 1333 Query: 167 AGDKIASLERSLNLYRDKYQSSLSNIELLECQVKMLQGELGGIMGQEPENKGDHSKVRIY 226 A +K E + + + +++ + L++ ++K + E+ + Q + V++ Sbjct: 1334 ASEK ESCITQLKKELSENINAVTLMKEELKEKKVEISSLSKQLTD LNVQLQ 1384 Query: 227 TSPCMIQEHQETQKRLSEVWQKVSQQDDLIQELRNKLACSNALVLEREKALIKLQADFAS 286 Ξ + ++ + ++ + D +Q+L K+ + L E+ AL ++ D+++ Sbjct: 1385 NSISLSEKEAAISSLRKQYDEEKCELLDQVQDLSFKV DTLSKEKISALEQVD-DWSN 1440 Query: 287 CTATHRYPPSS—SEECEDIKKILKHLQEQKDSQCLHVEEYQNLVKD LRVE-LE 337 + + S ++ +K++ L E K + +E NL+K+ R + L+ Sbjct: 1441 KFSEWKKKAQSRFTQHQNTVKELQIQL-ELKSKEAYEKDEQINLLKEELDQQNKRFDCLK 1499 Query: 338 AVSEQKRNIM-KDMMKLELDLHGLRE ETSAHIERKDKDITILQCRLQEL-QLEFTET 392 E ++ M K LE +L E HI +K +1 L L+ Q + E Sbjct: 1500 GEMEDDKSKMEKKESNLETELKSQTARIMELEDHITQKTIEIESLNEVLKNYNQQKDIEH 1559 Query: 393 QKLTLKKDKFLQ EKDEMLQELEKKLTQVQNSLLKKEKELEKQQCMATELEMTVKEAK 449 ++L K F + EKD ++E E+K+ ++N + + ELE ++ + ++VK Sbjct: 1560 KELVQKLQHFQELGEEKDNRVKEAEEKILTLENQVYSMKAELETKKKELEHVNLSVK 1616 Query: 450 QDKSKEAECKALQAEVQKLKNSLEEAKQQERLAAQQAAQCKEEAALAGCHLEDTQRKLQK 509 SKE E KAL+ ++ S + + +R A Q+ A K++ +E+ + + +K Sbjct: 1617 SKEEELKALEDRLES—ESAAKLAELKRKAEQKIAAIKKQLL SQMEEKEEQYKK 1668 Query: 510 GLLLDKQKADT-IQELQRELQMLQKESSMAEKEQTSNRKRVEELSLELSEALRKLENSDK 568 G + +T +QE +RE+ +L+++ E Q+ + S + A + E +D Sbjct: 1669 GTESHLSELNTKLQEREREVHILEEKLKSVESSQSETL—IVPRSAKNVAAYTEQEEADS 1726 Query: 569 E KRQLQK-TVAEQDMKMND-MLDRIKHQHREQGSIKCKLEEDLQEATKLLEDKREQ 622 + K +K +V ++++ + +L R+ Q +E+ ++ E Q +L+ K E Sbjct: 1727 QGCVQKTYEEKISVLQRNLTEKEKLLQRVG-QEKEE-TVSΞHFEMRCQYQERLI—KLEH 1782 Query: 623 LKKSKEHE-KLMEGEL-EALRQEFKKKDKTLKENSRKLEEENENLRAELQCCSTQLESSL 680 + +K+HE + M G L E L ++ KK + ++ K E N++A+ LE Sbjct: 1783 AE-AKQHEDQSMIGHLQEELEEKNKKYSLIVAQHVEK-EGGKNNIQAK QNLE 1832 Query: 681 NKYNTSQQVIQDLNKEIALQKESLMSLQAQLDKAL—QKEKHYLQTTITKEAYDALSR-K 737 N ++ Q+ +Q+ KE+ Q L +LD L QKE H ++ Y+ L + Sbjct: 1833 NVFDDVQKTLQE—KELTCQ—ILEQKIKELDSCLVRQKEVHRVEMEELTSKYEKLQALQ 1888 Query: 738 SAACQDDLTQALEKLNHVTSETKSLQQSLTQTQEKKAQ-LEEEIIAYEERMKKLNTEL— 794 ++ T+ LE+ S++ +Q L E + LE ++ E +KL E+ Sbjct: 1889 QMDGRNKPTELLEENTEEKSKSHLVQPKLLSNMEAQHNDLEFKLAGAEREKQKLGKEIVR 1948 Query: 795 —RKLRGFHQESELEVHAFDKKLEEMSCQVLQWQKQHQNDLKMLAAKEEQLREFQEEMAA 852 + LR +E + E+ K+ ++ + ++ Q+Q +LK + ++ +REF ++A Sbjct: 1949 LQKDLRMLRKEHQQELEILKKEYDQEREEKIK-QEQEDLELKHNSTLKQLMREFNTQLAQ 2007 Query: 853 LKENLLEDDKEPCCLPQWSVPKDTCRLYRGNDQIMTNLEQWAKQQKVANEKLGNQLREQV 912 ++ L KE Q V + + Q TN Q K K+A EK + R Sbjct: 2008 KEQELEMTIKETINKAQ-EVEAELLESH QEETN—QLLK--KIA-EKDDDLKRTAK 2057 Query: 913 NYIAKLSGEKDHLHSVMVHLQQENKKLKKEIEEKKMKAEN 952 Y L ++ + + + LQ + ++L+K+ ++K + EN Sbjct: 2058 RYEEILDAREEEMTAKVRDLQTQLEELQKKYQQKLEQEEN 2097 Score = 300 (45.0 bits), Expect = 3.7e-22, P = 3.7e-22 Identities = 195/961 (20%), Positives = 435/961 (45%) Query: 1 MKDEAGERDREVΞSLNSKLLSLQLDIKN—LHDVCKRQRKTLQDNQLCMEEAMNSSHDKK 58 +KD+ + +N K L +LD+K L + + L+ +EE ++ D+ Sbjct: 657 LKDKEIIFQAHIEEMNEKTLE-KLDVKQTELESLSSELSEVLKARHK-LEEELSVLKDQT 714 Query: 59 QAQALAFEESEVEFGSSKQCHLRQLQQLKKKLLV-LQQELEFHTEELQTΞYYSLRQYQSI 117 +E E + K H +Q+ + K+ V +Q+ + +++ L++ Sbjct: 715 DKMK QELEAKMDEQKNHHQQQVDSIIKEHEVSIQRTEKALKDQINQLELLLKERDKH 771 Query: 118 LEKQTSDLVLLHHHCKLKEDEVILYEEEMG NHNENTGEKLHLAQEQLALAGDKIASL 174 L++ + + L K E E+ ++ ++ T E+ +EQLA K+ L Sbjct: 772 LKEHQAHVENLEADIKRSEGELQQASAKLDVFQSYQSATHEQTKAYEEQLAQLQQKLLDL 831 Query: 175 ERSLNLYRDKYQSSLSNIELLECQVKMLQGELGGIMGQ-EPENKGDHSKVRIYTSPCMIQ 233 E L + + + + ++ + ++ +M Q E +N KV+ T Sbjct: 832 ETERILLTKQVAEVEAQKKDVCTELDAHKIQVQDLMQQLEKQNSEMEQKVKSLTQ-VYEΞ 890 Query: 234 EHQETQKRLSEVWQKVSQQDDLIQELRN KLACSNALVLEREKALIKLQADFASCTA 289 + ++ K + Q + +++++I ++R ++ + +E ++ L ++ + Sbjct: 891 KLEDGNKEQEQTKQILVEKENMILQMREGQKKEIEILTQKLSAKEDSIHILNEEYET 947 Query: 290 THRYPPSSSEECEDIKKILKHLQEQKDSQCLHVEEYQNLVKDLRVELEAVSEQKRNIMKD 349 ++ + ++ E +K+ K +QE + L E L K+L +S++++ Sbjct: 948 —KFK-NQEKKMEKVKQKAKEMQETLKKKLLDQEA—KLKKELENTALELSQKEKQFNAK 1002 Query: 350 MMKL-ELDLHGLREETSA-HIERKDKDITILQCRLQELQLEFTETQKLTLKKDKFLQEKD 407 M+++ + + G+ + S +K++ ++ + +EL + +K ++ + LQE Sbjct: 1003 MLEMAQANSAGIΞDAVΞRLETNQKEQIESLTEVHRRELNDVIΞIWEKKLNQQAEELQEIH 1062 Query: 408 EM-LQELEKKLTQVQNSLLK KEKELEKQQCMATE LEMTVKEAKQD-KSKEAEC 458 E+ LQE E+++ +++ +L +++E+ K+ E + T+ E ++ K K A Sbjct: 1063 EIQLQEKEQEVAELKQKILLFGCEKEEMNKEITWLKEEGVKQDTTLNELQEQLKQKSAHV 1122 Query: 459 KALQAEVQKLKNSLEEAKQQERLAAQQAAQCKEEAALAGCHLEDTQRKLQKGLLLDKQKA 518 +L + KLK LE+ + + ++ +E+ E+ +RK+ + L K K Sbjct: 1123 NSLAQDETKLKAHLEKLEVDLNKSLKENTFLQEQLVELKMLAEEDKRKVSE—LTSKLKT 1180 Query: 519 DTIQELQRELQMLQKESSMAEKEQTSNRKRVEELSLELSEALRKLENSDKEKRQLQKTVA 578 T +E Q +K + E + +K EEL+++L +K E + K + + Sbjct: 1181 -TDEEFQSLKSSHEKSNKSLEDKSLEFKKLSEELAIQLDICCKKTEALLEAKTN—ELIN 1237 Query: 579 EQDMKMNDMLDRIKH-QHREQGSIKCKLEEDLQEATKLLEDKREQLKKSKEHEKLMEGEL 637 K N +L Rl H QHR K++E L T + + QL++ E + + Sbjct: 1238 ISSSKTNAILSRISHCQHRTT KVKEALLIKTCTVSELEAQLRQLTEEQNTLNISF 1292 Query: 638 EALRQEFKKKD KTLKENSRKLEEENENLR AELQCCSTQLESSL 680 + + ++K+ K++K + L E E L+ +E + C TQL+ L Sbjct: 1293 QQATHQLEEKENQIKSMKADIESLVTEKEALQKEGGNQQQAASEKESCITQLKKELSENI 1352 Query: 681 NKYNTSQQVIQDLNKEIALQKESLMSLQAQLDKALQ-KEKHYLQTTITKEAYDALSRKSA 739 N ++ +++ EI+ + L L QL ++ EK +++ K+ YD + Sbjct: 1353 NAVTLMKEELKEKKVEISSLSKQLTDLNVQLQNSISLSEKEAAISSLRKQ-YDEEKCELL 1411 Query: 740 ACQDDLTQALEKLN-HVTSETKSLQQSLTQTQEKKAQLEEEIIAYEERMKKLNTELR-KL 797 DL+ ++ L+ S + + + E K + + ++ +K+L +L K Sbjct: 1412 DQVQDLSFKVDTLSKEKISALEQVDDWSNKFSEWKKKAQSRFTQHQNTVKELQIQLELKS 1471 Query: 798 RGFHQESELEVHAFDKKLEEMSCQVLQWQKQHQNDLKMLAAKEEQLR-EFQEEMAALKEN 856 + +++ E +++ ++L++ + + + + ++D + KE L E + + A + E Sbjct: 1472 KEAYEKDE-QINLLKEELDQQNKRFDCLKGEMEDDKSKMEKKEΞNLETELKΞQTARIME- 1529 Query: 857 LLEDDKEPCCLPQWSVPKDTCRLYRGNDQIMTNLEQWAKQQKVANEKLGNQLREQVNYIA 916 LED + + T + N+ ++ N Q QK K +L +++ + Sbjct: 1530 -LEDH ITQKTIEIESLNE-VLKNYNQ QKDIEHK ELVQKLQHFQ 1570 Query: 917 KLSGEKDH LHSVMVHLQQENKKLKKEIEEKKMKAENTRLCTKA 959 +L EKD+ ++ L+ + +K E+E KK + E+ L K+ Sbjct: 1571 ELGEEKDNRVKEAEEKILTLENQVYSMKAELETKKKELEHVNLSVKS 1617 Score = 298 (44.7 bits), Expect = 6.1e-22, P = 6.1e- 2 2 Identities = 207/886 (23%), Positives = 412/886 (46%) Query: 47 MEEAMNSSHDKKQAQALAFEESEVEFGSSKQCHLRQLQQLKKKLLVLQQELEFHTEELQT 106 + E N+ + Q EE E + S K ++ L + LQ+E + Sbjct: 1281 LTEEQNTLNISFQQATHQLEEKENQIKSMKA DIESLVTEKEALQKEGGNQQQAASE 1336 Query: 107 SYYSLRQYQSILEKQTSDLVLLHHHCKLKEDEVILYEEEMGNHNENTGEKLHLAQEQLAL 166 + Q + L + + + L+ K K+ E+ +++ + N + L++++ A Sbjct: 1337 KESCITQLKKELSENINAVTLMKEELKEKKVEISSLΞKQLTDLNVQLQNSISLSEKEAA- 1395 Query: 167 AGDKIASLERSLNLYRDKYQSSLSNIELLECQVKMLQGELGGIMGQEPENKGDHSKVRIY 226 I+SL + Y ++ L ++ L +V L E + Q + S+ + Sbjct: 1396 ISSLRKQ YDEEKCELLDQVQDLSFKVDTLSKEKISALEQVDDWSNKFSEWK-K 1447 Query: 227 TSPCMIQEHQETQKRLS EVWQKVSQQDDLIQEL—RNK-LACSNALVLE 272 + +HQ T K L E ++K Q + L +EL +NK C + + Sbjct: 1448 KAQSRFTQHQNTVKELQIQLELKSKEAYEKDEQINLLKEELDQQNKRFDCLKGEMEDDKS 1507 Query: 273 -REKALIKLQADFASCTAT HRYPPSSSEECEDIKKILKHLQEQKDSQCLHVEEYQN 327 EK L+ + Ξ TA + + E E + ++LK+ +QKD E++ Sbjct: 1508 KMEKKESNLETELKSQTARIMELEDHITQKTIEIESLNEVLKNYNQQKDI EHKE 1561 Query: 328 LVKDLRVELEAVSEQKRNIMKDMMKLELDLHGLREETSAHIERKDKDI—TILQCRLQEL 385 LV+ L+ + + E+K N +K+ + L L A +E K K++ L + +E Sbjct: 1562 LVQKLQ-HFQELGEEKDNRVKEAEEKILTLENQVYSMKAELETKKKELEHVNLSVKSKEE 1620 Query: 386 QLEFTETQKLTLKKDKFLQEKDEMLQELEKKLTQVQNSLLKKEKELEKQQCMATELEMTV 445 +L+ E + L+ + + E+ ++ E+K+ ++ LL + +E E+Q TE ++ Sbjct: 1621 ELKALEDR LESES-AAKLAELKRKAEQKIAAIKKQLLSQMEEKEEQYKKGTESHLSE 1676 Query: 446 KEAKQDKSKEAECKALQAEVQKLKNSLEEAKQQERLAAQQAAQCK-EEAALAGCHLEDTQ 504 K + +E E L+ +++ +++S E R A AA + EEA GC + + Sbjct: 1677 LNTKLQE-REREVHILEEKLKSVESSQSETLIVPRSAKNVAAYTEQEEADSQGCVQKTYE 1735 Query: 505 RKLQKGLLLDKQKADTIQELQRELQMLQKESSMAEKEQTSNRKRVEELSLELSEALRKLE 564 K+ +L + + + LQR Q +KE +++ + R + +E ++L A K Sbjct: 1736 EKIS VLQRNLTEKEKLLQRVGQ--EKEETVSSHFEM--RCQYQERLIKLEHAEAKQH 1788 Query: 565 NSDKEKRQLQKTVAEQDMKMNDMLDRIKHQHREQG—SIKCK—LE EDLQ E 611 LQ+ + E++ K + ++ +H +E G +1+ K LE +D+Q E Sbjct: 1789 EDQSMIGHLQEELEEKNKKYSLIV—AQHVEKEGGKNNIQAKQNLENVFDDVQKTLQEKE 1846 Query: 612 AT-KLLEDKREQLKKSKEHEKLMEG-ELEALRQEFKKKDKTLKENSR KLEEENENL 665 T ++LE K ++L +K + E+E L +++K + + R +L EEN Sbjct: 1847 LTCQILEQKIKELDSCLVRQKEVHRVEMEELTSKYEKLQALQQMDGRNKPTELLEENTEE 1906 Query: 666 RAELQCCSTQLESSLN-KYNTSQQVIQDLNKEIALQKESLMSLQAQLDKALQKEKHYLQT 724 +++ +L S++ ++N + + +E + ++ LQ L + L+KE H + Sbjct: 1907 KSKSHLVQPKLLΞNMEAQHNDLEFKLAGAEREKQKLGKEIVRLQKDL-RMLRKE-HQQEL 1964 Query: 725 TITKEAYDALSRKSAACQDDLTQALEKLNHVTSETKSLQQSLTQTQEKKAQLEEEIIAYE 784 I K+ YD R+ Q+ + LE L H ++ + +++ TQ +K+ +LE I + Sbjct: 1965 EILKKEYDQ-EREEKIKQEQ—EDLE-LKHNSTLKQLMREFNTQLAQKEQELEMTI K 2017 Query: 785 ERMKKLNTELRKLRGFHQESELEVHAFDKKLEEMSCQVLQWQKQHQNDLKMLAAKEEQLR 844 E + K +L HQE E + KK+ E + + K+++ ++L A+EE++ Sbjct: 2018 ETINKAQEVEAELLESHQE ETNQLLKKIAEKDDDLKRTAKRYE EILDAREEEMT 2071 Query: 845 EFQEEMAALKENLLEDDKEPCCLPQWSVP-KDTCRLYRGNDQIMTNLEQWAKQQKVANEK 903 ++ E L + ++ L Q P D + ++ T L Q K +++ K Sbjct: 2072 AKVRDLQTQLEELQKKYQQK—LEQEENPGNDNVTIM ELQTQLAQ—KTTLISDSK 2123 Query: 904 LGNQ-LREQVNYIA-KLSGEKDHLHSVMV-HL 932 L Q REQ++ + +L + ++++ V HL Sbjct: 2124 LKEQEFREQIHNLEDRLKKYEKNVYATTVGHL 2155 Score = 280 (42.0 bits), Expect = 5.2e-20, P = 5.2e-20 Identities = 209/938 (22%), Positives = 432/938 (46%) Query: 3 DEAGERDREVS-SLNSKLLSLQLDIKN-LHDVC-KRQRKTLQDNQLCMEEAM-NSSHDKK 58 ++ ++ +E+ +L KLL + +K L + + +K Q N +E A NS+ Sbjct: 957 EKVKQKAKEMQETLKKKLLDQEAKLKKELENTALELSQKEKQFNAKMLEMAQANΞAGISD 1016 Query: 59 QAQALAFEESEVEFGSSKQCHLRQLQQLKKKLLVLQQELEFHTEELQTSYYSLRQYQSIL 118 L + E + S + H R+L + + + +++L EELQ + ++ + Sbjct: 1017 AVSRLETNQKE-QIESLTEVHRRELNDV ISIWEKKLNQQAEELQ-EIHEIQLQEK— 1069 Query: 119 EKQTSDLV—LLHHHCKLKE-DEVILYEEEMGNHNENTGEKLHLAQEQLALAGDKIASLE 175 E++ ++L +L C+ +E ++ I + +E G + T +L +Q + + +A E Sbjct: 1070 EQEVAELKQKILLFGCEKEEMNKEITWLKEEGVKQDTTLNELQEQLKQKSAHVNSLAQDE 1129 Query: 176 RSLNLYRDKYQSSLSNIELLECQVKMLQGELGGI--MGQEPENKGDHSKVRIYTSPCMIQ 233 L + +K + L N L E LQ +L + + +E + K ++ T+ Q Sbjct: 1130 TKLKAHLEKLEVDL-NKSLKENT—FLQEQLVELKMLAEEDKRKVSELTSKLKTTDEEFQ 1186 Query: 234 E HQETQKRLSEVWQKVSQQDDLIQELRNKL--AC—SNALVLEREKALIKLQADFA 285 H+++ K L + K + L +EL +L C + AL+ + LI + + Sbjct: 1187 SLKSSHEKSNKSLED KSLEFKKLSEELAIQLDICCKKTEALLEAKTNELINISSSKT 1243 Query: 286 SCTATH-RYPPSSSEECEDIKKILKHLQEQKDΞQCLHVEEYQNLVKDLRVELEAVSEQKR 344 + + + + + ++ I + ++Q + E QN + + E+K Sbjct: 1244 NAILSRISHCQHRTTKVKEALLIKTCTVSELEAQLRQLTEEQNTLNISFQQATHQLEEKE 1303 Query: 345 NIMKDMMKLELD-LHGLREETSAHIERKDKDITILQCRLQELQLEFTET-QKLTLKKDKF 402 N +K M K +++ L +E + + + + + +L+ E +E +TL K++ Sbjct: 1304 NQIKSM-KADIESLVTEKEALQKEGGNQQQAASEKESCITQLKKELSENINAVTLMKEE- 1361 Query: 403 LQEKDEMLQELEKKLTQVQNSLLKKEKELEKQQCMATELEMTVKEAKQDKSKEAECKALQ 462 L+EK + L K+LT + N L+ L +++ + L E K + + + L Sbjct: 1362 LKEKKVEISSLSKQLTDL-NVQLQNSISLSEKEAAISΞLRKQYDEEKCELLDQVQ—DLS 1418 Query: 463 AEVQKLKNSLEEAKQQERLAAQQAAQCKEEAALAGCHLEDTQRKLQKGLLLDKQKA 518 +V L A +Q + + ++ K++A ++T ++LQ L L ++A Sbjct: 1419 FKVDTLSKEKISALEQVDDWSNKFSEWKKKAQSRFTQHQNTVKELQIQLELKSKEAYEKD 1478 Query: 519 DTIQELQRELQMLQKESSMAEKEQTSNRKRVEELSLELSEALRKLENSDKEKRQLQKTVA 578 + I L+ EL K + E ++ ++E+ L +L++ +L+ + Sbjct: 1479 EQINLLKEELDQQNKRFDCLKGEMEDDKSKMEKKESNLET ELKSQTARIMELEDHIT 1535 Query: 579 EQDMKMNDMLDRIKHQHREQGSIKCK-LEEDLQEATKLLEDKREQLKKSKEHEKLMEGEL 637 ++ +++ + + +K+ + +Q 1+ K L + LQ +L E+K ++K+++E +E ++ Sbjct: 1536 QKTIEIESLNEVLKN-YNQQKDIEHKELVQKLQHFQELGEEKDNRVKEAEEKILTLENQV 1594 Query: 638 EALRQEFKKKDKTLKENSRKLEEENENLRAELQCCSTQLES-SLNKYNTSQQVIQDLNKE 696 +++ E + K K L+ + ++ + E L+A L+ +LES S K ++ + ++ Sbjct: 1595 YSMKAELETKKKELEHVNLSVKSKEEELKA-LE DRLESESAAKL AELKRKAEQK 1647 Query: 697 IALQKESLMSLQAQLDKALQKEKHYLQTTITKEAYDALΞRKSAACQDDLTQALEKLNHVT 756 IA K+ L+S Q++ +KE+ Y + T + L+ K + ++ EKL V Sbjct: 1648 IAAIKKQLLS QME EKEEQYKKGT--ESHLSELNTKLQEREREVHILEEKLKSVE 1699 Query: 757 S ET KSLQQSLTQTQEKKAQLEEEII-AYEERMKKLNTELRKLRGFHQESELEV 808 S ET +S + T++++A + + YEE++ L L E E + Sbjct: 1700 SSQSETLIVPRSAKNVAAYTEQEEADSQGCVQKTYEEKISVLQRNLT EKEKLL 1752 Query: 809 HAFDKKLEEMSCQVLQWQKQHQNDLKMLAAKEEQLREFQEEMAALKENLLEDDKEPCCLP 868 ++ EE + + Q+Q L L E + E Q + L+E L E +K+ + Sb j ct: 1753 QRVGQEKEETVSSHFEMRCQYQERLIKLEHAEAKQHEDQSMIGHLQEELEEKNKKYSLIV 1812 Query: 869 QWSVPKDTCRLYRGNDQIMTNLEQ-WAKQQKVANEK-LGNQLREQ-VNYIAKLSGEKDHL 925 V K+ + N Q NLE + QK EK L Q+ EQ + + + + Sbjct: 1813 AQHVEKEGGK NNIQAKQNLENVFDDVQKTLQEKELTCQILEQKIKELDSCLVRQKEV 1869 Query: 926 HSV-MVHLQQENKKLK 940 H V M L + +KL+ Sbjct: 1870 HRVEMEELTSKYEKLQ 1885 Score = 227 (34.1 bits), Expect = 2.5e-14, P = 2.5e-14 Identities = 160/716 (22%), Positives = 318/716 (44%) Query: 233 QEHQETQKRLSEVWQKVSQQDDLIQE-LRNKLACSNALV-LEREKALIKL-QADFASCTA 289 +E +TQ ++ +V + L + ++ L S++ L R + L + D S TA Sbjct: 53 RESGDTQSFAQKLQLRVPSVESLFRSPIKESLFRSSSKESLVRTSSRESLNRLDLDSSTA 112 Query: 290 THRYPPSSSEECEDIKKILKHLQEQKDSQCLHVEEYQNLVKDLRVELEAVSEQKRNIMKD 349 + P E ED+ L +++ Q L + + R + + + + ++ Sbjct: 113 SFDPPSDMDSEAEDLVGNΞDSLNKEQLIQRLR—RMERSLSSYRGKYSELVTAYQMLQRE 170 Query: 350 MMKLELDLHGLREETSAHIERKDKDIT-ILQCRLQELQLEFTETQKLTLKKDKFLQEKDE 408 KL+ G+ ++ +DK + I + R +ELQ++ + L + D L+EKD+ Sbjct: 171 KKKLQ GILSQS QDKSLRRIAELR-EELQMDQQAKKHLQEEFDASLEEKDQ 219 Query: 409 MLQELEKKLTQVQNSLLKKEKELEKQQCMATELEMTVKEAKQDKSKEAECKALQAE V 465 + L+ +++ ++ L ++ + + +LE + ++++ E++ + + + V Sb j ct: 220 YISVLQTQVSLLKQRLRNGPMNVDVLKPLP-QLEPQAEVFTKEENPESDGEPVVEDGTΞV 278 Query: 466 QKLKNSLEEAKQQERLA—AQQAAQC-KEEAALAGCHLEDTQRKLQKGLL-LDKQKADTI 521 + L+ + K+QE L ++ Q KE+ L E Q +L + L L+K K + Sb j ct: 279 KTLETLQQRVKRQENLLKRCKETIQSHKEQCTLLTSEKEALQEQLDERLQELEKIKDLHM 338 Query: 522 QELQRELQMLQKESSMAEKEQTSNRKRVEELSLELSEALRKLENΞDKEKRQLQKTVAEQD 581 E + + L+ ++ E+ + + E ++ E L E + R K + Q Sbjct: 339 AEKTKLITQLRDAKNLIEQLEQDKGMVIAETKRQMHETLEMKEEEIAQLRSRIKQMTTQG 398 Query: 582 MKMNDMLDRIKHQHREQGSIKCKLEEDLQEAT-KLLEDKREQLK KSKEHEKL-MEGE 636 ++ + ++ + E+ + +EA KL + EQ+K K+ E E++ ++ E Sbjct: 399 EELREQKEKSERAAFEELEKALSTAQKTEEARRKLKAEMDEQIKTIEKTSEEERISLQQE 458 Query: 637 LEALRQEFKK-KDKTLKENSRKLEEENENLRAELQCCSTQLESSLNKYNTSQQVIQDLNK 695 L ++QE K+ +E KL++ +E EL +L L T ++ Q+ K Sbjct: 459 LSRVKQEVVDVMKKSΞEEQIAKLQKLHEK ELARKEQELTKKLQ TREREFQEQMK 512 Query: 696 EIALQKESLMSLQAQLDKALQKEKHYLQTTITKEAYDALSRKSAACQDDLTQALEKLN-H 754 +AL+K L+ +K Q+ + + K+A S DL Q E Sbjct: 513 -VALEKSQSEYLKISQEKEQQESLALEELELQKKAILTESENKLR DLQQEAETYRTR 568 Query: 755 VTSETKSLQQSLTQTQEKKAQLEEEIIAYEERMKKLNTELRKLRGFHQESELEV—HAFD 812 + SL++SL QE K Q ++ + E K N E+ + H+ +ELE H D Sbjct: 569 ILELESSLEKSL QENKNQSKDLAVHLEAEKNKHNKEITVMVEKHK-TELESLKHQQD 624 Query: 813 KKLEEMSCQVLQWQKQHQNDLKMLAAKEEQLRE FQEEMAALKENLLED-DK 862 E QVL+ +Q+Q +++ L K EQ +E FQ + + E LE D Sbjct: 625 ALWTE-KLQVLK—QQYQTEMEKLREKCEQEKETLLKDKEIIFQAHIEEMNEKTLEKLDV 681 Query: 863 EPCCLPQWSVPKDTCRLYRGNDQIMTNLEQWAKQQKVANEKLGNQLREQVNYIAKLSGEK 922 + L S+ + + + ++ L Q ++L ++ EQ N+ + Sbjct: 682 KQTELE—SLSSELSEVLKARHKLEEELSVLKDQTDKMKQELEAKMDEQKNHHQQQVDSI 739 Query: 923 DHLHSVMVHLQQENKKLKKEIEEKKM 948 H V + Q+ K LK +1 + ++ Sbjct: 740 IKEHEVSI—QRTEKALKDQINQLEL 763 Score = 183 (27.5 bits), Expect = 1.3e-09, P = 1.3e-09 Identities = 132/584 (22%), Positives = 251/584 (42%) Query: 409 MLQELEKKLTQVQNSLLKKEKELEKQQCMATELEMTVKEAK-QDKSKEAECKALQAEVQK 467 M ++L++K+++ Q L + + +T M + + ++ E + Q Sbjct: 1 MFKKLKQKISEEQQQLQQALAPAQASSNSSTPTRMRSRTSSFTEQLDEGTPNRESGDTQS 60 Query: 468 LKNSLE-EAKQQERLAAQQAAQCKEEAALAGCHLEDTQRKLQKGLLLDKQKA—DTIQEL 524 L+ E L + ++ + + R+ L LD A D ++ Sbjct: 61 FAQKLQLRVPSVESLFRSPIKESLFRSSSKESLVRTSSRESLNRLDLDSΞTASFDPPSDM 120 Query: 525 QRELQMLQKESSMAEKEQTSNRKRVEELSL ELSEALRKLENSDKEKRQLQKTVAE 579 E + L S KEQ R R E SL + SE + + +EK++LQ +++ Sbjct: 121 DSEAEDLVGNSDSLNKEQLIQRLRRMERSLSSYRGKYSELVTAYQMLQREKKKLQGILSQ 180 Query: 580 -QDMKMNDMLDRIKHQHREQGSIKCKLEE DLQEATK LLEDKREQLKKSKEHEKL 632 QD + + + + +Q + K EE L+E + +L+ + LK+ + + Sbjct: 181 SQDKSLRRIAELREELQMDQQAKKHLQEEFDASLEEKDQYISVLQTQVSLLKQRLRNGPM 240 Query: 633 MEGELEALRQ-EFKKKDKTLKENSRKLEE ENENLRAELQCCSTQLESSLNKYNTSQQ 688 L+ L Q E + + T +EN E E+ L+ +++ N ++ Sbjct: 241 NVDVLKPLPQLEPQAEVFTKEENPESDGEPVVEDGTSVKTLETLQQRVKRQENLLKRCKE 300 Query: 689 VIQDLNKEIALQKESLMSLQAQLDKALQKEKHYLQTTITKEAYDALSRKSAACQDDLTQA 748 IQ ++ L +LQ QLD+ LQ E ++ E +++ A +L + Sbjct: 301 TIQSHKEQCTLLTSEKEALQEQLDERLQ-ELEKIKDLHMAEKTKLITQLRDA—KNLIEQ 357 Query: 749 LEK-LNHVTSETKSLQQSLTQTQEKKAQLEEEIIAYEERMKKLNTELRKLRGFHQESELE 807 LE+ V +ETK + + +T E K EEEI R+K++ T+ +LR Q+ + E Sbjct: 358 LEQDKGMVIAETK RQMHETLEMK EEEIAQLRSRIKQMTTQGEELR—EQKEKSE 409 Query: 808 VHAFDKKLEEMSCQVLQWQKQHQNDLKMLAAKEEQLREFQ EEMAALKENLLEDDKE 863 AF EE+ + QK + K+ A +EQ++ + EE +L++ L +E Sbjct: 410 RAAF EELEKALΞTAQKTEEARRKLKAEMDEQIKTIEKTSEEERISLQQELSRVKQE 465 Query: 864 PCCLPQWSVPKDTCRLYRGNDQIMTNLEQ-WAKQQKVANEKLGNQLR EQVNYIAK 917 + + S + +L + +++ + EQ K+ + + Q++ Q Y+ K Sbjct: 466 VVDVMKKSSEEQIAKLQKLHEKELARKEQELTKKLQTREREFQEQMKVALEKSQSEYL-K 524 Query: 918 LSGEKDHLHSVMVH-LQQENKKLKKEIEEK KMKAENTRLCTKALGPSRTESTQREK 972 +S EK+ S+ + L+ + K + E E K + +AE R L S +S Q K Sbjct: 525 ISQEKEQQESLALEELELQKKAILTESENKLRDLQQEAETYRTRILELESSLEKSLQENK 584 Pedant information for DKFZphtes3_lgl3, frame 1 Report for DKFZphtes3_lgl3.1 [LENGTH] 1007 [MW] 117480.77 [pi] 5.90 [HOMOL] TREMBL:AF092090_1 product: "cpl51"; Rattus norvegicus cpl51 mRNA, partial eds. 0.0 [FUNCAT) 30.03 organization of cytoplasm [S. cerevisiae, YDL058w] 5e-15 [FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YDL058w] 5e-15 [FUNCAT] 09.10 nuclear biogenesis [S. cerevisiae, YDR356w] le-11 [FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YDR356w] le-11 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YDR356w] le-11 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YKR095w] le-08 [FUNCAT] 11.04 dna repair (direct repair, base excision repair and nucleotide excision repair) [S. cerevisiae, YKR095w] le-08 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YLR309c] le-08 [FUNCAT] 1 genome replication, transcription, recombination and repair [M. annaschn, MJ1322] 4e-06 [FUNCAT] 09.13 biogenesis of chromosome structure [S. cerevisiae, YLR086w] 9e-06 [FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YHR023w MYOl - myosm-1 isoform] 3e-04 [FUNCAT] 08.22 cytoskeleton-dependent transport [S. cerevisiae, YHR023w MYOl - myosιn-1 isoform] 3e-04 [FUNCAT] 03.25 cytokinesis [S. cerevisiae, YHR023w MYOl - myosιn-1 isoform] 3e-04 [FUNCAT] 98 classification not yet clear-cut [S. cerevisiae, YJR134c] 5e-04 [EC] 3.6.1.32 Myosin ATPase le-16 [PIRKW] nucleus 3e-10 [PIRKW] phosphotransferase 6e-09 [PIRKW] duplication 2e-06 [PIRKW] citrullme 2e-12 [PIRKW] tandem repeat le-16 [PIRKW] endocytosis 2e-13 [PIRKW] heart 8e-13 [PIRKW] transmembrane protein le-13 [PIRKW] serine/threonine-specific protein kinase 6e-09 [PIRKW] zmc fmger 2e-13 [PIRKW] metal binding 2e-13 [PIRKW] DNA binding 4e-12 [PIRKW] muscle contraction le-16 [PIRKW] acetylated ammo end le-11 [PIRKW] actin binding le-16 [PIRKW] mitosis 5e-15 [PIRKW] microtubule binding 5e-15 [PIRKW] ATP le-16 [PIRKW] thick filament le-16 [PIRKW] phosphoprotein 4e-16 [PIRKW] skeletal muscle 2e-14 [PIRKW] calcium binding 2e-12 [PIRKW] alternative splicing le-16 [PIRKW] coiled coil le-16 [PIRKW] P-loop le-16 [PIRKW] heptad repeat 3e-10 [PIRKW] methylated amino acid le-16 [PIRKW] lmmunoglobulin receptor 2e-06 [PIRKW] peripheral membrane protein 2e-13 [PIRKW] cardiac muscle 8e-13 [PIRKW] hydrolase le-16 [PIRKW] microtubule 3e-10 [PIRKW] muscle 8e-13 [PIRKW] EF hand 2e-12 [PIRKW] cytoskeleton 2e-15 [PIRKW] hair 2e-12 [PIRKW] calmodulin binding 2e-13 [PIRKW] Golgi apparatus 3e-10 [SUPFAM] myosin heavy chain le-16 [SUPFAM] conserved hypothetical P115 protein le-07 [SUPFAM] centromere protein E 5e-15 [SUPFAM] unassigned Ser/Thr or Tyr-specific protein kinases 6e-09 [SUPFAM] calmodulin repeat homology 2e-12 [SUPFAM] myosin motor domain homology le-16 [SUPFAM] alpha-actimn actin-bmdmg domain homology 2e-07 [SUPFAM] plect 2e-07 [SUPFAM] trichohyalin 2e-12 [SUPFAM] pleckstrm repeat homology 8e-08 [SUPFAM] ribosomal protein S10 homology 2e-07 [SUPFAM] giantin 3e-13 [SUPFAM] protein kinase homology 6e-09 [SUPFAM] protein kinase C zmc-bmding repeat homology 8e-08 [SUPFAM] kinesin motor domain homology 5e-15 [SUPFAM] human early endosome antigen 1 2e-13 [SUPFAM] M5 protein le-07 [PROSITE] LEUCINE_ZIPPER 7 [PROSITE] MYRISTYL 2 [PROSITE] CAMP_PHOSPHO_SITE 2 [PROSITE] CK2 PHOSPHO_SITE 20 [PROSITE] TYR_PHOSPHO_SITE 1 [PROSITE] PKC_PHOSPHO_SITE 16 [PROSITE] ASN_GLYCOSYLATION 2 [KW] All_Alpha [KW] LOW_COMPLEXITY 15.00 % [KW] COILED COIL 42.40 % SEQ MKDEAGERDREVSSLNSKLLSLQLDIKNLHDVCKRQRKTLQDNQLCMEEAMNSSHDKKQA SEG xxxxxxxxxxxx PRD ccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCC SEQ QALAFEESEVEFGΞSKQCHLRQLQQLKKKLLVLQQELEFHTEELQTSYYSLRQYQSILEK SEG xxxxxxxxxxxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC SEQ QTSDLVLLHHHCKLKEDEVILYEEEMGNHNENTGEKLHLAQEQLALAGDKIASLERSLNL SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC SEQ YRDKYQSSLSNIELLECQVKMLQGELGGIMGQEPENKGDHSKVRIYTSPCMIQEHQETQK SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCCC SEQ RLSEVWQKVSQQDDLIQELRNKLACSNALVLEREKALIKLQADFASCTATHRYPPSSSEE SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS SEQ CEDIKKILKHLQEQKDSQCLHVEEYQNLVKDLRVELEAVΞEQKRNIMKDMMKLELDLHGL SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC SEQ REETSAHIERKDKDITILQCRLQELQLEFTETQKLTLKKDKFLQEKDEMLQELEKKLTQV SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCC CCCCCCCCCCCCCC SEQ QNSLLKKEKELEKQQCMATELEMTVKEAKQDKSKEAECKALQAEVQKLKNSLEEAKQQER SEG ... xxxxxxxxxx xxxxxxxx PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC SEQ LAAQQAAQCKEEAALAGCHLEDTQRKLQKGLLLDKQKADTIQELQRELQMLQKESSMAEK SEG xxxxxxxxxxxxxxxx xxxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCC SEQ EQTSNRKRVEELSLELSEALRKLENSDKEKRQLQKTVAEQDMKMNDMLDRIKHQHREQGS SEG xxxxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC SEQ IKCKLEEDLQEATKLLEDKREQLKKSKEHEKLMEGELEALRQEFKKKDKTLKENSRKLEE SEG xxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCC SEQ ENENLRAELQCCSTQLEΞSLNKYNTSQQVIQDLNKEIALQKESLMSLQAQLDKALQKEKH SEG xxxxxxxxx PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCC CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC SEQ YLQTTITKEAYDALSRKSAACQDDLTQALEKLNHVTSETKSLQQSLTQTQEKKAQLEEEI SEG xxxxxxxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCCCCCC SEQ IAYEERMKKLNTELRKLRGFHQESELEVHAFDKKLEEMSCQVLQWQKQHQNDLKMLAAKE SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCCCCCC SEQ EQLREFQEEMAALKENLLEDDKEPCCLPQWSVPKDTCRLYRGNDQIMTNLEQWAKQQKVA SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS SEQ NEKLGNQLREQVNYIAKLSGEKDHLHSVMVHLQQENKKLKKEIEEKKMKAENTRLCTKAL SEG xxxxxxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC SEQ GPSRTESTQREKVCGTLGWKGLPQDMGQRMDLTKYIGMPHCPGSSYC SEG PRD cchhhhhhhhhhhhhhhhcccccccccchhhhhheeecccccccccc COILS Prosite for DKFZphtes3_lgl3.1 PS00001 52->56 ASN_GLYCOSYLATION PDOC00001 PS00001 684->688 ASN_GLYCOSYLATION PDOC00001 PS00004 240->244 CAMP_PHOSPHO_SITE PDOC00004 PS00004 415->419 CAMP_PHOSPHO_SITE PDOC00004 PS00005 74->77 PKC_PHOSPHO_SITE PDOC00005 PS00005 110->113 PKC_PHOSPHO_SITE PDOC00005 PS00005 238->241 PKC_PHOSPHO_ΞITE PDOC00005 PS00005 290->293 PKC_PHOΞPHO_SITE PDOC00005 PS00005 392->395 PKC_PHOSPHO_SITE PDOC00005 PS00005 396->399 PKC_PHOSPHO_SITE PDOC00005 PS00005 444->447 PKC_PHOSPHO_SITE PDOC00005 PS00005 503->506 PKC_PHOSPHO_SITE PDOC00005 PS00005 544->547 PKC_PHOSPHO_SITE PDOC00005 PS00005 566->569 PKC_PHOSPHO_SITE PDOC00005 PS00005 600->603 PKC_PHOSPHO_SITE PDOC00005 PS00005 650->653 PKC_PHOSPHO_SITE PDOC00005 PS00005 655->658 PKC_PHOSPHO_SITE PDOC00005 PS00005 735->738 PKC_PHOSPHO_SITE PDOC00005 PS00005 876->879 PKC_PHOSPHO_SITE PDOC00005 PS00005 968->971 PKC_PHOSPHO_SITE PDOC00005 PS00006 39->43 CK2_PHOSPHO_SITE PDOC00006 PS00006 53->57 CK2_PHOSPHO_SITE PDOC00006 PS00006 68->72 CK2_PHOSPHO_SITE PDOC00006 PS00006 116-M20 CK2_PHOSPHO_SITE PDOC00006 PS00006 190->194 CK2_PHOSPHO_SITE PDOC00006 PS00006 250->254 CK2_PHOSPHO_SITE PDOC00006 PS00006 296->300 CK2_PHOSPHO_SITE PDOC00006 PS00006 439->443 CK2_PHOSPHO_SITE PDOC00006 PS00006 444->448 CK2_PHOSPHO_SITE PDOC00006 PS00006 471->475 CK2_PHOSPHO_SITE PDOC00006 PS00006 520->524 CK2_PHOSPHO_SITE PDOC00006 PS00006 536->540 CK2_PHOSPHO_SITE PDOC00006 PΞ00006 566->570 CK2_PHOSPHO_SITE PDOC00006 PS00006 576->580 CK2_PHOSPHO_SITE PDOC00006 PS00006 650->654 CK2_PHOSPHO_SITE PDOC00006 PS00006 674->678 CK2_PHOSPHO_SITE PDOC00006 PS00006 804->808 CK2_PHOSPHO_SI E PDOC00006 PS0000Θ 888->892 CK2_PHOSPHO_SITE PDOC00006 PS00006 963->967 CK2_PHOSPHO_SITE PDOC00006 PS00006 968->972 CK2_PHOSPHO_SITE PDOC00006 PS00007 135->143 TYR_PHOSPHO_SITE PDOC00007 PΞ00008 207->213 MYRISTYL PDOC00008 PS00008 599->605 MYRISTYL PDOC00008 PS00029 83->105 LEUCINE_ZIPPER PDOC00029 PS00029 90->112 LEUCINE_ZIPPER PDOC00029 PS00029 97->119 LEUCINE_ZIPPER PDOC00029 PS00029 104->126 LEUCINE_ZIPPER PDOC00029 PS00029 403->425 LEUCINE_ZIPPER PDOC00029 PS00029 410->432 LEUCINE_ZIPPER PDOC00029 PS00029 918->940 LEUCINE ZIPPER PDOC00029 (No Pfam data available for DKFZphtes3_lgl3.1) DKFZphtes3_lkll group: cell structure and motility DKFZphtes3_lkll encodes a novel 589 amino acid protein with strong similarity to Mus musculus actin-binding protein (ENC-1) . Ectoderm-neural cortex-1 protein (ENC-1) is an early and highly specific marker of neural induction in vertebrates. The protein is related to the kelch family proteins and is expressed during early gastrulation in the prospective neuroectodermal region of the epiblast and later m development throughout the nervous system (NS) . ENC-1 functions as an actin-binding protein organising the actin cytoskeleton during neural differentiation and development of the NS . The novel protein is highly similar to ENC-1. The new protein can find application in modulation of cyto skeleton organisation in human testicular cells. strong similarity to mouse ENC-1 complete cDNA, compete eds, EST hits Sequenced by DKFZ Locus: unknown Insert length: 3525 bp Poly A stretch at pos. 3515, polyadenylation signal at pos. 3499 1 GGTGGAGAGC CGGCCGACGG GAGCCGCGGC GGAGCCTGTT GAGCTCGCGC 51 GGGCTGCCGG GAGTGGTCTC TGAGGCGGCG GCGGCGGCGG GGATCGTCTC 101 CGGCACTGGC GCACCATGTC GGTCAGTGTC CATGAGACCC GCAAGTCGCG 151 GAGCAGCACG GGGTCCATGA ACGTCACCCT CTTCCACAAG GCCTCCCACC 201 CGGACTGTGT GCTGGCCCAC CTCAACACGC TTCGCAAGCA CTGCATGTTC 251 ACCGACGTCA CACTCTGGGC GGGCGACCGT GCCTTCCCCT GTCACCGTGC 301 CGTGCTGGCC GCCTCTAGCC GCTATTTTGA GGCCATGTTC AGCCATGGCC 351 TTCGGGAGAG CCGGGATGAC ACTGTCAACT TCCAGGACAA CCTGCACCCG 401 GAGGTGCTGG AGCTGCTGCT GGACTTTGCC TACTCCTCAC GCATCGCCAT 451 CAACGAGGAG AACGCTGAGT CACTGCTGGA GGCAGGCGAC ATGCTGCAGT 501 TCCACGATGT GCGGGATGCT GCCGCCGAGT TCCTGGAGAA GAACCTTTTC 551 CCCTCCAACT GCCTGGGCAT GATGCTGCTC TCGGACGCCC ACCAGTGCCG 601 CCGGCTGTAT GAGTTCTCCT GGCGCATGTG CCTGGTGCAC TTTGAGACGG 651 TGAGGCAGAG CGAGGACTTC AACAGCCTGT CCAAGGACAC ACTGCTGGAC 701 CTCATCTCGA GTGATGAGCT GGAGACCGAG GACGAGCGGG TGGTCTTCGA 751 GGCCATCCTC CAGTGGGTGA AGCACGACCT GGAGCCACGG AAGGTCCACT 801 TGCCCGAGCT CCTCCGCAGC GTGCGTCTGG CCTTGCTGCC GTCCGACTGC 851 CTGCAGGAGG CCGTCTCCAG CGAGGCCCTC CTCATGGCAG ACGAGCGCAC 901 CAAGCTTATC ATGGATGAGG CCCTGCGCTG CAAGACCAGG ATCCTGCAGA 951 ATGATGGCGT GGTCACCAGC CCCTGTGCCC GGCCACGCAA GGCGGGCCAC 1001 ACGCTACTCA TCCTGGGGGG CCAGACCTTC ATGTGTGACA AGATCTACCA 1051 GGTGGACCAC AAGGCCAAGG AGATCATCCC CAAGGCCGAC CTGCCCAGCC 1101 CCCGGAAGGA GTTCAGCGCC TCAGCGATCG GCTGCAAGGT CTATGTGACG 1151 GGGGGCAGGG GCTCCGAGAA CGGGGTCTCC AAGGATGTCT GGGTGTACGA 1201 CACCGTACAT GAGGAATGGT CCAAGGCGGC GCCCATGCTG ATTGCCCGCT 1251 TTGGCCATGG CTCAGCTGAG CTGGAGAACT GCCTCTATGT GGTGGGGGGA 1301 CACACATCCC TGGCAGGGGT CTTCCCGGCC TCGCCTTCTG TCTCCCTGAA 1351 ACAAGTGGAG AAATACGACC CTGGGGCCAA CAAGTGGATG ATGGTGGCCC 1401 CCTTGCGGGA TGGCGTCAGC AATGCCGCAG TGGTGAGTGC CAAGCTGAAG 1451 CTCTTTGTTT TCGGAGGAAC CAGCATCCAC CGGGACATGG TGTCCAAGGT 1501 CCAGTGCTAT GACCCCTCGG AGAACAGGTG GACGATCAAG GCCGAGTGCC 1551 CCCAGCCTTG GCGGTACACA GCCGCTGCCG TCCTGGGCAG CCAGATCTTC 1601 ATCATGGGAG GTGACACGGA ATTCACAGCC GCCTCGGCCT ACCGCTTTGA 1651 CTGTGAGACC AACCAGTGGA CGCGGATTGG GGACATGACT GCCAAGCGCA 1701 TGTCCTGCCA TGCCCTGGCT TCCGGCAACA AGCTCTATGT GGTCGGGGGC 1751 TACTTTGGGA CCCAGAGGTG TAAGACTCTG GACTGCTATG ACCCCACTTC 1801 AGATACATGG AACTGCATCA CCACAGTGCC CTACTCACTT ATCCCCACGG 1851 CCTTTGTCAG CACCTGGAAG CACCTGCCCG CGTGAGGAGC ACCTGCTGAG 1901 CCCAGCCAGA CCGCGGCCTT CAGTGTCACA GCGTGGCCTT GCTTGTCTGC 1951 CACAGCGGGA GCTAAGCCGG CCCTGGGCCA GCACTCCGAG AGGTGGAAGG 2001 GGCCCTGCCA GCTCTGGGGA GCAGCAGCCT TGGGCTGTTC TGAGCTTTAG 2051 GCAAGAGAAG AGAAGCATCT CTTGCATCCG TGCCCCTGGG GGCCTCTTCA 2101 GCTTTGCAGT GGTTTGTGGG AAGACATACC TCCCAGAGGG GCATGGACTG 2151 CCACCAGGAC TGACCCTGGC GTCGGGGAGA AGGACACTTG CAGAGCCTTG 2201 AGATCACCTG TTTGGCAGGT CCTGGACTGG GGCCGGGCAG GCAGGGGCAG 2251 GGAGGCGCCC CGGGTGGGCT TTGGGGCTGC GGCACTGCCA CACATCCTTT 2301 CCCTCCTGGC CTGCCCTGCT GGGGCTCTAC TGCCATCTAT AGATGGTGTC 2351 CTGGGCCTGG GAAACTAGGT TCCCAGGGGT TGAGACCAGA AAGGTGACCA 2401 AGACAGATTT TTTAAGGTGC AGAAACTGCA GGGGGGCCTC AGTGACATCC 2451 ATGAGGCCTT ATTAGCAAAG GACACCCAGA CCTCCAAGGT TTGTGGGCCC 2501 CTTCCACAAA GCTGTAAGTC CCAGCCCACC TACTCAGGGC CTTGCTCAGT 2551 GCTGTGGCCC GGTGGGGACA CAGTTGCTCG TGGCCACTCA GTGGAGCTGG 2601 GCCTGCAGCA GACTCAAGGC TCCGAGTGCC CTGGGGGTCA CCCCTCCCCT 2651 CCCCTCCTCA GAGCCCACCC TGAGAGGCAG CAGTGACCCC CATGGCACAC 2701 ACCTGCCAAC AGCACTGGGG GCTTCTCCCC AGGAGACCAC GCTGCCCTCC 2751 AAGACCAGGA GCAGCTGTGA GCTGGAGACA GCAGAGGGAC CCCAGGGTGT 2801 CCCCTGCAGA TCCCACCAGG GCCGCATCCA TCTCAGTGTG GAGGACAGTG 2851 ACGGGACCCT CACCATCCTC TTGCGTTTTG GCCCCCATTT GCTCCCTGAG 2901 CTCCAAGATA AGAATGGCCC CGAGAGAACT GCTGAACATT TGTTCATTGC 2951 TGTCACCTCC TGAGTCACTG GGGTCCCTCA CCAGCACCTC CCTGACACCT 3001 GGGCTATGGA GAGGTTGGCG CCTGTCAGTG ACCATCCTAA TGCCTCTCGC 3051 TCACTCCCAA GCCACCATTT GAGAGGGAGG GGTGTTGGTG CCCTGACAGG 3101 GACTGGGCAG GGTGTCCAAA CTTGGGGCTT CCCAGGCACC TGCAGTGTGA 3151 ACACTGCTTG GCTGGCTCAA GATTAGGGCC GCGGAGGGGG CTGTGCACAT 3201 ACCAGTTACT TAAGCAGCCA CGAGTGTCCC CCATGCCTTG GTGCGGGTCC 3251 TGGAGGCCTC TTGGGGGTGG GACCTTTGGG CAGGGTTTGC CCACTGACGC 3301 GCCCGCCATG GGGCACTGGC TGCATGGGGC TCCTTGGACC CTGTAGACCC 3351 AGCAGGAGCC TGGCCGCGGG GACTGCAGGG AGGGTGCCTG GACCCGTGGG 3401 GTTGCTTCAT TGAGATAAAG CACACTTATC ACATAGCACA AAGGACGTGC 3451 CATGGTGCTT TCCCCAAAAG TTGTGTTGCT TTTATCAGTT TTCTAACTTA 3501 ATAAAAAGAG TTGAGAAAAA AAAAA BLAST Results No BLAST result Medline entries 98350113: Cloning of human ENC-1 and evaluation of its expression and regulation in nervous system tumors. 97252647: ENC-1: a novel mammalian kelch-related gene specifically expressed in the nervous system encodes an actin-bmdmg protein. 98234394: NRP/B, a novel nuclear matrix protein, associates with pllO(RB) and is involved in neuronal differentiati Peptide information for frame 2 ORF from 116 bp to 1882 bp; peptide length: 589 Category: strong similarity to known protein Classification: Cell structure/motility 1 MSVSVHETRK SRSSTGSMNV TLFHKASHPD CVLAHLNTLR KHCMFTDVTL 51 WAGDRAFPCH RAVLAASSRY FEAMFSHGLR ESRDDTVNFQ DNLHPEVLEL 101 LLDFAYSSRI AINEENAESL LEAGDMLQFH DVRDAAAEFL EKNLFPSNCL 151 GMMLLSDAHQ CRRLYEFSWR MCLVHFETVR QSEDFNSLSK DTLLDLISSD 201 ELETEDERVV FEAILQWVKH DLEPRKVHLP ELLRSVRLAL LPSDCLQEAV 251 SSEALLMADE RTKLIMDEAL RCKTRILQND GVVTSPCARP RKAGHTLLIL 301 GGQTFMCDKI YQVDHKAKEI IPKADLPSPR KEFSASAIGC KVYVTGGRGS 351 ENGVSKDVWV YDTVHEEWSK AAPMLIARFG HGSAELENCL YVVGGHTSLA 401 GVFPASPSVS LKQVEKYDPG ANKWMMVAPL RDGVSNAAVV SAKLKLFVFG 451 GTSIHRDMVS KVQCYDPSEN RWTIKAECPQ PWRYTAAAVL GSQIFIMGGD 501 TEFTAASAYR FDCETNQWTR IGDMTAKRMS CHALASGNKL YVVGGYFGTQ 551 RCKTLDCYDP TSDTWNCITT VPYSLIPTAF VSTWKHLPA BLASTP hits Entry MMU65079__1 from database TREMBL: gene: "ENC-1"; product: "actin-bindmg protein"; Mus musculus actin-binding protein (ENC-1) mRNA, complete eds. Score = 2402, P = 1.9e-249, identities = 440/589, positives = 513/589 Entry AF059611_1 from database TREMBLNEW: gene: "NRPB"; product: "nuclear matrix protein NRP/B"; Homo sapiens nuclear matrix protein NRP/B (NRPB) mRNA, complete eds. Score = 2400, P = 3.0e-249, identities = 440/589, positives = 512/589 Entry AF010314_1 from database TREMBL: gene: "PIG10"; product: "PiglO"; Homo sapiens PiglO (PIG10) mRNA, complete eds . Score = 1745, P = 7.8e-180, identities = 335/507, positives = 403/507 Entry KELC_DROME from database SWISSPROT: RING CANAL PROTEIN (KELCH PROTEIN) . >TREMBL : DMRCPA_1 product: "ring canal protein"; Drosophila melanogaster ring canel protein and ORF2 mRNA, complete eds. Score = 672, P = 3.9e-66, identities = 168/536, positives = 257/536 Alert BLASTP hits for DKFZphtes3_lkll, frame 2 No Alert BLASTP hits found Pedant information for DKFZphtes3_lkll, frame 2 Report for DKFZphtes3_lkll .2 [LENGTH] 589 [MW] 65923.45 [pi] 6.10 [HOMOL] TREMBL:MMU65079_1 gene: "ENC-1"; product: "actm-binding protein"; Mus musculus actm-binding protein (ENC-1) mRNA, complete eds. 0.0 [FUNCAT] 10.05.99 other pheromone response activities [S. cerevisiae, YHR158c] 2e-09 [BLOCKS] BL01016D Glycoprotease family proteins [PIRKW] zmc f ger le-08 [PIRKW] DNA binding le-08 [PIRKW] transcription factor le-08 [SUPFAM] POZ domain homology 3e-68 [SUPFAM] vaccinia virus 59K Hindlll-C protein le-15 [SUPFAM] A55R protein 5e-29 [SUPFAM] hypothetical protein YHR158c 4e-08 [SUPFAM] A55R protein middle region homology 5e-29 [SUPFAM] myxoma virus M9-R protein le-14 [SUPFAM] A55R protein carboxyl-terminal homology 5e-29 [KW] Alpha_Beta SEQ MSVSVHETRKSRSSTGSMNVTLFHKASHPDCVLAHLNTLRKHCMFTDVTLWAGDRAFPCH PRD cccccccccccccccccceeeeeeccccchhhhhhhhhhhhhhhhheeeeeecccchhhh SEQ RAVLAASSRYFEAMFSHGLRESRDDTVNFQDNLHPEVLELLLDFAYSSRIAINEENAESL PRD hcccccccccccccccccchhhhhheeeeccccchhhhhhhhhhhhccceeehhhhhhhh SEQ LEAGDMLQFHDVRDAAAEFLEKNLFPSNCLGMMLLSDAHQCRRLYEFSWRMCLVHFETVR PRD hhhhhhhhhhhhhhhhhhhhhhhccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhh SEQ QSEDFNSLSKDTLLDLISSDELETEDERVVFEAILQWVKHDLEPRKVHLPELLRSVRLAL PRD hhhhhhhhhhhhhhhhhhcccccchhhhhhhhhhhhhhhhhccchhhhhhhhhhhhhhhc SEQ LPSDCLQEAVSSEALLMADERTKLIMDEALRCKTRILQNDGVVTSPCARPRKAGHTLLIL PRD ccchhhhhhhhhhhhhcccchhhhhhhhhhhhhhhhcccccccccccccccccceeeeee SEQ GGQTFMCDKIYQVDHKAKEIIPKADLPSPRKEFSASAIGCKVYVTGGRGSENGVSKDVWV PRD cccccccceeeeeccccccccccccccccccceeeeeeceeeeeecccccccccceeeee SEQ YDTVHEEWSKAAPMLIARFGHGΞAELENCLYVVGGHTSLAGVFPASPΞVSLKQVEKYDPG PRD cccccccccccccccccccccceeeccceeeeecccccccccccccccccccceeecccc SEQ ANKWMMVAPLRDGVΞNAAVVSAKLKLFVFGGTSIHRDMVSKVQCYDPSENRWTIKAECPQ PRD ccceeeeccccccccceeeeeccceeeeeccccccccccceeeecccccccccccccccc SEQ PWRYTAAAVLGSQIFIMGGDTEFTAASAYRFDCETNQWTRIGDMTAKRMSCHALASGNKL PRD ccccceeeeecceeeeecccccccccceeecccccccceeeccccccccceeeeecccee SEQ YVVGGYFGTQRCKTLDCYDPTSDTWNCITTVPYSLIPTAFVSTWKHLPA PRD eeecccccccccccccccccccccceeeeeccccccceeeeeecccccc (No Prosite data available for DKFZphtes3_lkll .2) (No Pfam data available for DKFZphtes3 lkll.2) DKFZphtes3_ln3 group: signal transduction DKFZphtes3_ln3 encodes a novel 1196 ammo acid protein with similarity to S. pombe Tupl protein. The protein contains 1 WD-40 repeat, which is typical for the beta-transducin subunit of G- protems . The beta subunits seem to be required for the replacement of GDP by GTP as well as for membrane anchoring and receptor recognition. In addition, a RGD site is present. The new protein can find application in modulating/blocking G-protein-dependent pathways. similarity to Tuplp complete cDNA, complete eds, EST hits Sequenced by DKFZ Locus: /map="6q24" Insert length: 5277 bp Poly A stretch at pos. 5267, polyadenylation signal at pos. 5244 1 GCTGCATAAA GCTGAGAGAT GCCTACAGCT GAGAGTGAAG CAAAAGTAAA 51 AACCAAAGTT CGCTTTGAAA AATTGCTTAA GACCCACAGT GATCTAATGC 101 GTGAAAAGAA AAAACTGAAG AAAAAACTTG TCAGGTCTGA AGAAAACATC 151 TCACCTGACA CTATTAGAAG CAATCTTCAC TATATGAAAG AAACTACAAG 201 TGATGATCCC GACACTATTA GAAGCAATCT TCCCCATATT AAAGAAACTA 251 CAAGTGATGA TGTAAGTGCT GCTAACACTA ACAACCTGAA GAAGAGCACG 301 AGAGTCACTA AAAACAAATT GAGGAACACA CAGTTAGCAA CTGAAAATCC 351 TAATGGTGAT GCTAGTGTAG AGGAAGACAA ACAAGGAAAG CCAAATAAAA 401 AGGTGATAAA GACGGTGCCC CAGTTGACTA CACAAGACCT GAAACCGGAA 451 ACTCCTGAGA ATAAGGTTGA TTCTACACAC CAGAAAACAC ATACAAAGCC 501 ACAGCCAGGC GTTGATCATC AGAAAAGTGA GAAGGCAAAT GAGGGAAGAG 551 AAGAGACTGA TTTAGAAGAG GATGAAGAAT TGATGCAAGC ATATCAGTGC 601 CATGTAACTG AAGAAATGGC AAAGGAGATT AAGAGGAAAA TAAGAAAGAA 651 ACTGAAAGAA CAGTTGACTT ACTTTCCCTC AGATACTTTA TTCCATGATG 701 ACAAACTAAG CAGTGAAAAA AGGAAAAAGA AAAAGGAAGT TCCAGTCTTC 751 TCTAAAGCTG AAACAAGTAC ATTGACCATC TCTGGTGACA CAGTTGAAGG 801 TGAACAAAAG AAAGAATCTT CAGTTAGATC AGTTTCTTCA GATTCTCATC 851 AAGATGATGA AATAAGCTCA ATGGAACAAA GCACAGAAGA CAGCATGCAA 901 GATGATACAA AACCTAAACC AAAAAAAACA AAAAAGAAGA CTAAAGCAGT 951 TGCAGATAAT AATGAAGATG TTGATGGTGA TGGTGTTCAT GAAATAACAA 1001 GCCGAGATAG CCCGGTTTAT CCCAAATGTT TGCTTGATGA TGACCTTGTC 1051 TTGGGAGTTT ACATTCACCG AACTGATAGA CTTAAGTCAG ATTTTATGAT 1101 TTCTCACCCA ATGGTAAAAA TTCATGTGGT TGATGAGCAT ACTGGTCAAT 1151 ATGTCAAGAA AGATGATAGT GGACGGCCTG TTTCATCTTA CTATGAAAAA 1201 GAGAATGTGG ATTATATTCT TCCTATTATG ACCCAGCCAT ATGATTTTAA 1251 ACAGTTAAAA TCAAGACTTC CAGAGTGGGA AGAACAAATT GTATTTAATG 1301 AAAATTTTCC CTATTTGCTT CGAGGCTCTG ATGAGAGTCC TAAAGTCATC 1351 CTGTTCTTTG AGATTCTTGA TTTCTTAAGC GTGGATGAAA TTAAGAATAA 1401 TTCTGAGGTT CAAAACCAAG AATGTGGCTT TCGGAAAATT GCCTGGGCAT 1451 TTCTTAAGCT TCTGGGAGCC AATGGAAATG CAAACATCAA CTCAAAACTT 1501 CGCTTGCAGC TATATTACCC ACCTACTAAG CCTCGATCCC CATTAAGTGT 1551 TGTTGAGGCA TTTGAATGGT GGTCAAAATG TCCAAGAAAT CATTACCCAT 1601 CAACACTGTA CGTAACTGTA AGAGGACTGA AAGTTCCAGA CTGTATAAAG 1651 CCATCTTACC GCTCTATGAT GGCTCTTCAG GAGGAAAAAG GTAAACCAGT 1701 GCATTGTGAA CGTCACCATG AGTCAAGCTC AGTAGACACA GAACCTGGAT 1751 TAGAAGAGTC AAAGGAAGTA ATAAAGTGGA AACGACTCCC TGGGCAGGCT 1801 TGCCGTATCC CAAACAAACA CCTCTTCTCA CTAAATGCAG GAGAACGAGG 1851 ATGTTTTTGT CTTGATTTCT CCCACAATGG AAGAATATTA GCAGCAGCTT 1901 GTGCCAGCCG GGATGGATAT CCAATTATTT TATATGAAAT TCCTTCTGGA 1951 CGTTTCATGA GAGAATTGTG TGGCCACCTC AATATCATTT ATGATCTTTC 2001 CTGGTCAAAA GATGATCACT ACATCCTTAC TTCATCATCT GATGGCACTG 2051 CCAGGATATG GAAAAATGAA ATAAACAATA CAAATACTTT CAGAGTTTTA 2101 CCTCATCCTT CTTTTGTTTA CACGGCTAAA TTCCATCCAG CTGTAAGAGA 2151 GCTAGTAGTT ACAGGATGCT ATGATTCCAT GATACGGATA TGGAAAGTTG 2201 AGATGAGAGA AGATTCTGCC ATATTGGTCC GACAGTTTGA TGTTCACAAA 2251 AGTTTTATCA ACTCACTTTG TTTTGATACT GAAGGTCATC ATATGTATTC 2301 AGGAGATTGT ACAGGGGTGA TTGTTGTTTG GAATACCTAT GTCAAGATTA 2351 ATGATTTGGA ACATTCAGTG CACCACTGGA CTATAAATAA GGAAATTAAA 2401 GAAACTGAGT TTAAGGGAAT TCCAATAAGT TATTTGGAGA TTCATCCCAA 2451 TGGAAAACGT TTGTTAATCC ATACCAAAGA CAGTACTTTG AGAATTATGG 2501 ATCTCCGGAT ATTAGTAGCA AGGAAGTTTG TAGGAGCAGC AAATTATCGG 2551 GAGAAGATTC ATAGTACTTT GACTCCATGT GGGACTTTTC TGTTTGCTGG 2601 AAGTGAGGAT GGTATAGTGT ATGTTTGGAA CCCAGAAACA GGAGAACAAG 2651 TAGCCATGTA TTCTGACTTG CCATTCAAGT CACCCATTCG AGACATTTCT 2701 TATCATCCAT TTGAAAATAT GGTTGCATTC TGTGCATTTG GGCAAAATGA 2751 GCCAATTCTT CTGTATATTT ACGATTTCCA TGTTGCCCAG CAGGAGGCTG 2801 AAATGTTCAA ACGCTACAAT GGAACATTTC CATTACCTGG AATACACCAA 2851 AGTCAAGATG CCCTATGTAC CTGTCCAAAA CTACCCCATC AAGGCTCTTT 2901 TCAGATTGAT GAATTTGTCC ACACTGAAAG TTCTTCAACG AAGATGCAGC 2951 TAGTAAAACA GAGGCTTGAA ACTGTCACAG AGGTGATACG TTCCTGTGCT 3001 GCAAAAGTCA ACAAAAATCT CTCATTTACT TCACCACCAG CAGTTTCCTC 3051 ACAACAGTCT AAGTTAAAGC AGTCAAACAT GCTGACCGCT CAAGAGATTC 3101 TACATCAGTT TGGTTTCACT CAGACCGGGA TTATCAGCAT AGAAAGAAAG 3151 CCTTGTAACC ATCAGGTAGA TACAGCACCA ACGGTAGTGG CTCTTTATGA 3201 CTACACAGCG AATCGATCAG ATGAACTAAC CATCCATCGC GGAGACATTA 3251 TCCGAGTGTT TTTCAAAGAT AATGAAGACT GGTGGTATGG CAGCATAGGA 3301 AAGGGACAGG AAGGTTATTT TCCAGCTAAT CATGTGGCTA GTGAAACACT 3351 GTATCAAGAA CTGCCTCCTG AGATAAAGGA GCGATCCCCT CCTTTAAGCC 3401 CTGAGGAAAA AACTAAAATA GAAAAATCTC CAGCTCCTCA AAAGCAATCA 3451 ATCAATAAGA ACAAGTCCCA GGACTTCAGA CTAGGCTCAG AATCTATGAC 3501 ACATTCTGAA ATGAGAAAAG AACAGAGCCA TGAGGACCAA GGACACATAA 3551 TGGATACACG GATGAGGAAG AACAAGCAAG CAGGCAGAAA AGTCACTCTA 3601 ATAGAGTAAA GAATTGAAGA AAAGTTAAGA GCTGCCGAAA TGCACAGAGG 3651 TGAAAATGAC AAACCAAATG GAATTTCTCT TCAGAGTTCA GAATTTTCAG 3701 ATACTAAGGA GGAAGAAAGG ATCCACTACT TCTTGTTCTT ATGAATGACT 3751 CTAGAAAAAT CAGAATCAAG TTGTGGGTGG AAAAATCAAC GTGGCCTTTG 3801 AGTTCAGTTG TTATAAACCA TTGTGACTAT TGTTGGTCAA AGTATTGGTA 3851 CTTATATTGT TAGTAATTGC ATCATAATTA CATTACCAGT GTTGGAAAAC 3901 TAATGAAGAA AACACTGTAA TTGCTACTCA GCAAATGTGA ATAAAAGGTG 3951 TTTGCGTTAT TAGGATGTCT GTTAAGTAAT CATTTAATAT TATTATATTG 4001 GTAATGGTTG TATGTGTGAT GCTATGCCCA GAATATGAAG TATCTGTTTT 4051 TGAAATTCAC TTTATTTAAA AGATAAGCAG CTGACTGGGC ACGGTGCCTC 4101 ATGCCTGTAA TCCTAGCACC TTGGGAGGCT GAGGCAGGTG GATCACCTAA 4151 GGTCAGGAGT TCAACAACAC CAGCCTGACC AACATGGTGA AACCCCATCT 4201 CTACTAAAAA TACAAAAATC AGCCGGGTCT CATGGCAGGC ACCTGTAATC 4251 CCATCTACTG AGGCAGGAGA ATTGCTTGAC CCAGGAGGCA GAGGTTGCAG 4301 TGAGCCAAGA TCACGCCATT GCACTCCAGC CTGGGGGACA GAGCAAGACT 4351 CTATCTCCAA AAAACAAAAA AGATAAGCAG CTTTAGAATA TGGCGCATTC 4401 AAAACAGTCT CAGTAACAAA GACATTAAAA GAAAACAATT TACTTTCTAA 4451 TTAAAATTTT GTGTTTCTTA AGATCAAATC ATATAGGTAA CTTCATAGAC 4501 CTAAATTAAA AGTGATTTTT GGCTGGACTG GCAACAATGT TCCCAATGTC 4551 TTTACTTTTT AAAAAAGGCT TTTCATATTT AAGCACATAC CTATTTTGTA 4601 GACTTACATT GTTTAATATT TATTTTAATC TTAATATTTT TACATTATTA 4651 TATTGCATTA TTTATTTTTT CTAAGTTCCA GAATAATAGT GTCATTATTA 4701 TAGACTATAT GTTTTGAAGT TTGATATTAT AATGGGATAT TCATTTTTTG 4751 TTCTTTTCTT GACTCCTTTC TCAAGTGTGT GATAAGGTCT GCTGATAAAA 4801 TATTTAACCC CAAGAAAGTG AAAACTAATA TAAAATTAGA AAGACCTATC 4851 CAAATTAGAC AGTCAATTCC ATTAAAATAA GAAGTGAGAA AAACAATGTT 4901 GGGCATTGAG GTGTAAATTT TGCCCAGATG TATACCCAGT GTGAAATATC 4951 TTCTAATAAA AATATATTTG GCTCTTATCC CTGCACATGT AGAGGCATAA 5001 AAATTGGTAA ACATGTCCCG CTGTGTAGAA CTTTAAAAAA AAGGCATTTT 5051 TGAAAGTGTT GAGTGGCACT GATAACTGGT GAAGCCTACA GCCATCCGCC 5101 CAAAAGTCTG TTCTGATGGC ACTGAGTTTT CATTGTTCTG GATGTATAAG 5151 TCTGTGTGTC AGGTACAGCT GGGCCCAGCC AGCTTGAGTC ACTCTTGTAC 5201 AAGCTTGTTT TTTTCTGTCT TGTGAATGCA CTTGATAATT TAAAAATAAA 5251 AATATCTGTT TCTCTGCAAA AAAAAAA BLAST Results Entry HS32B1 from database EMBL: Human DNA sequence *** SEQUENCING IN PROGRESS *** from clone 32B1 Score = 4445, P = O.Oe+00, identities = 889/889 Entry U93816 from database EMBL: Human exon-trapped sequence from 6q24. Score = 965, P = 4.0e-35, identities = 193/193 Medline entries No Medline entry Peptide information for frame 1 ORF from 19 bp to 3606 bp; peptide length: 1196 Category: similarity to known protein 1 MPTAESEAKV KTKVRFEKLL KTHSDLMREK KKLKKKLVRS EENISPDTIR 51 SNLHYMKETT SDDPDTIRSN LPHIKETTSD DVSAANTNNL KKSTRVTKNK 101 LRNTQLATEN PNGDASVEED KQGKPNKKVI KTVPQLTTQD LKPETPENKV 151 DSTHQKTHTK PQPGVDHQKS EKANEGREET DLEEDEELMQ AYQCHVTEEM 201 AKEIKRKIRK KLKEQLTYFP SDTLFHDDKL SSEKRKKKKE VPVFSKAETS 251 TLTISGDTVE GEQKKESSVR SVSSDSHQDD EISSMEQSTE DSMQDDTKPK 301 PKKTKKKTKA VADNNEDVDG DGVHEITSRD SPVYPKCLLD DDLVLGVYIH 351 RTDRLKSDFM ISHPMVKIHV VDEHTGQYVK KDDSGRPVSS YYEKENVDYI 401 LPIMTQPYDF KQLKSRLPEW EEQIVFNENF PYLLRGSDES PKVILFFEIL 451 DFLSVDEIKN NSEVQNQECG FRKIAWAFLK LLGANGNANI NSKLRLQLYY 501 PPTKPRSPLS VVEAFEWWSK CPRNHYPSTL YVTVRGLKVP DCIKPSYRSM 551 MALQEEKGKP VHCERHHESS SVDTEPGLEE SKEVIKWKRL PGQACRIPNK 601 HLFSLNAGER GCFCLDFSHN GRILAAACAS RDGYPIILYE IPSGRFMREL 651 CGHLNIIYDL SWSKDDHYIL TSSSDGTARI WKNEINNTNT FRVLPHPSFV 701 YTAKFHPAVR ELVVTGCYDS MIRIWKVEMR EDSAILVRQF DVHKSFINSL 751 CFDTEGHHMY ΞGDCTGVIVV WNTYVKINDL EHSVHHWTIN KEIKETEFKG 801 IPISYLEIHP NGKRLLIHTK DSTLRIMDLR ILVARKFVGA ANYREKIHST 851 LTPCGTFLFA GSEDGIVYVW NPETGEQVAM YSDLPFKSPI RDISYHPFEN 901 MVAFCAFGQN EPILLYIYDF HVAQQEAEMF KRYNGTFPLP GIHQSQDALC 951 TCPKLPHQGS FQIDEFVHTE SSSTKMQLVK QRLETVTEVI RSCAAKVNKN 1001 LSFTSPPAVS SQQSKLKQSN MLTAQEILHQ FGFTQTGIIS IERKPCNHQV 1051 DTAPTVVALY DYTANRSDEL TIHRGDIIRV FFKDNEDWWY GSIGKGQEGY 1101 FPANHVASET LYQELPPEIK ERSPPLSPEE KTKIEKSPAP QKQSINKNKS 1151 QDFRLGSESM THSEMRKEQS HEDQGHIMDT RMRKNKQAGR KVTLIE BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_ln3, frame 1 TREMBL :U92792_1 gene: "tupl"; product: "Tupl"; Schizosaccharomyces pombe general transcriptional repressor Tupl (tupl) mRNA, complete eds., N = 1, Score = 186, P = le-10 TREMBL: AF104258_1 gene: "Pmc733"; product: "putative copper-mducible 35.6 kDa protein"; Festuca rubra putative copper-inducible 35.6 kDa protein (Pmc733) mRNA, complete eds., N = 1, Score = 235, P = 4.6e-18 TREMBL :SPAC3H5_8 gene: "SPAC3H5.08c"; product: "beta-transducm"; S. pombe chromosome I cosmid c3H5. , N = 2, Score = 231, P = 2e-14 PIR:T02533 hypothetical protein F13M22.17 - Arabidopsis thaliana, N = 2, Score = 228, P = le-13 TREMBL :AF104258_1 gene: "Pmc733"; product: "putative copper-inducible 35.6 kDa protein"; Festuca rubra putative copper-inducible 35.6 kDa protein (Pmc733) mRNA, complete eds., N = 1, Score = 235, P = 4.6e-18 TREMBL :SPAC3H5_8 gene: "SPAC3H5.08c"; product: "beta-transducin"; S. pombe chromosome I cosmid c3H5., N = 2, Score = 231, P = 2e-14 TREMBL :CER03E1_1 gene: "R03E1.1"; Caenorhabditis elegans cosmid R03E1, N = 1, Score = 215, P = 2.3e-13 SWISSPROT :YZLL_CAEEL HYPOTHETICAL 43.1 KD TRP-ASP REPEATS CONTAINING PROTEIN K04G11.4 IN CHROMOSOME X., N = 1, Score = 203, P = 7. le-13 >TREMBL:AF104258_1 gene: "Pmc733"; product: "putative copper-mducible 35.6 kDa protein"; Festuca rubra putative copper-mducible 35.6 kDa protein (Pmc733) mRNA, complete eds. Length = 321 HSPs: Score = 235 (35.3 bits), Expect = 4.6e-18, P = 4.6e-18 Identities = 59/225 (26%), Positives = 111/225 (49%) Query: 647 MRELCGHLNIIYDLSWSKDDHYILTSSSDGTARIWKNEINNTNTFRVLPHPSFVYTAKFH 706 + E GH + I DLSWSK+ +L++S D T R+W ++ + +V H ++V +F+ Sbjct: 63 VHEFYGHGDAILDLSWSKNGD-LLSASMDKTVRLW—QVGRDSCLKVFSHTNYVTCVQFN 119 Query: 707 PAVRELVVTGCYDSMIRIWKVEMREDSAILVRQFDVHKSFINSLCFDTEGHHMYSGDCTG 766 P +TGC D ++RIW V LV + K + ++C+ +G +G TG Sbjct: 120 PTNGNYFITGCIDGLVRIWDVRK CLVVDWANSKEIVTAVCYRPDGKGAVAGTITG 174 Query: 767 VIVVWNTYVKINDLEHSVHHWTINKEIKETEFKGIPISYLEIHPNGKRLLIHTKDSTLRI 826 ++ +LE V ++N K + + Y P K+L++ + D+ +RI Sbjct: 175 NCRYYDASENRLELESQV SLNGRKKSLHKRIVGFQYCPSDP—KKLMVTSGDAQVRI 229 Query: 827 MDLRILVARKFVGAANYREKIHSTLTPCGTFLFAGSEDGIVYVWN 871 +D +++ + G + ++ + TP G + + S+D +Y+WN Sbjct: 230 LDGAHVISN-YKGLQS-SSQVARSFTPDGDHIVSASDDΞRIYMWN 272 Pedant information for DKFZphtes3_ln3, frame 1 Report for DKFZphtes3_ln3.1 [LENGTH] 1196 [MW] 137114.70 [pi] 6.79 [HOMOL] SWISSPROT :YKY4_CAEEL HYPOTHETICAL 40.4 KD TRP-ASP REPEATS CONTAINING PROTEIN C14B1.4 IN CHROMOSOME III. 8e-21 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YKL121w] 2e-ll [FUNCAT] 04.05.01.01 general transcription activities [S. cerevisiae, YBR198c TAF90 - TFIID subunit] 4e-10 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YBR198C TAF90 - TFIID subunit] 4e-10 [FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YPR178w] le-08 [FUNCAT] 04.05.03 mrna processing (splicing) [S. cerevisiae, YPR178w] le-08 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YDR364c] 4e-08 [FUNCAT] 03.16 dna synthesis and replication [S. cerevisiae, YDR364c] 4e-08 [FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YDL145c] 9e-08 [FUNCAT] 30.09 organization of intracellular transport vesicles [S. cerevisiae, YDL145C] 9e-08 [FUNCAT] 04 . . 0 5.01.04 transcriptional control [S. cerevisiae, YCR084c] 2e-07 [FUNCAT] 10 . . 9 9 other signal-transduction activities [S. cerevisiae, YHL002w] 7e-07 [FUNCAT] 98 c.lassification not yet clear-cut [S. cerevisiae, YFR024c-a] 2e-06 [FUNCAT] 02 , . 1 6 fermentation [S. cerevisiae, YMR116c] 4e-06 [FUNCAT] 30 . . 0, 3 organization of cytoplasm [S. cerevisiae, YMR116c] 4e-06 [FUNCAT] 05 . . 0 4 translation (initiation, elongation and termination) [S. cerevisiae, YMR116C ] 4e-06 [FUNCAT] 03 , . 1 0 sporulation and germination [S. cerevisiae, YFL009w] 4e-05 [FUNCAT] 03 , . 0 4 budding, cell polarity and filament formation [S. cerevisiae, YFL009w] 4e-05 [FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YFL009w] 4e-05 [FUNCAT] 03.01 cell growth [S. cerevisiae, YCR088w] 6e-05 [FUNCAT] 03.25 cytokinesis [S. cerevisiae, YCR057c] 7e-05 [BLOCKS] BL00024H [SCOP] dltbgd_ 2.46 3.1.1 betal-subunit of the signal-transducmg 3e-91 [SCOP] dlgfc 2.21 2.1.9 Growth factor receptor-bound protein 2 (GRB2), N 4e-14 [SCOP] dlfmk_l 2.21 2.1.8 (1-64) c-src tyrosine kinase [human (Hom Se-15 [SCOP] dladδbl 2.21 2.1.7 (1-63) Hemapoetic cell kinase Hck [human (Hom 3e-15 [SCOP] dllckal 2.21 2.1.16 (1-54) p56-lck tyrosine kinase, SH3 domain [huma le-13 [SCOP] dlqwea_ 2.21 2.1.15 Src kinase, SH3 domain [Avian sarcoma virus 2e-15 [SCOP] dlshg_ 2.21 2.1.6 alpha-Spectnn, SH3 domain [chicken (Gallu 2e-13 [SCOP] dlprmc 2.21 2.1.13 Src kinase, SH3 domain [chicken (Gallus gallus) 2e-15 [SCOP] dlhsq_ 21.2.1.12 Phospholipase C, SH3 domain [human (Hom 2e-13 [SCOP] dlaboa 21.2.1.3 Abl tyrosine kinase, SH3 domain [Mouse (Mu 3e-13 [SCOP] dlefna 21.2.1.2 Fyn, SH3 domain [human (Homo sapiens) 2e-15 [SCOP] dlsema 21.2.1.11 Growth factor receptor-bound protein 2 (GRB2) , N le-13 [SCOP] dlgbqa 21.2.1.10 Growth factor receptor-bound protein 2 (GRB2), N 3e-16 [SCOP] dlckaa_ 21.2.1.1 C-Crk, N-terminal SH3 domain [mouse (Mu 3e-15 [EC] 3.1.4.3 Phospholipase C 2e-07 [EC] 3.1.4.11 l-Phosphatιdylιnosιtol-4, 5-bιsphosphate phosphodiesterase 7e-07 [EC] 3.6.1.32 Myosin ATPase 7e-07 [EC] 2 . 7 . 1 . 112 Protein-tyrosme kinase 8e-06 [PIRKW] nucleus 2e-08 [PIRKW] phosphotransferase 8e-06 [PIRKW] plasma 4e-07 [PIRKW] duplication 4e-07 [PIRKW] phosphoric diester hydrolase 2e-07 [PIRKW] tandem repeat 7e-07 [PIRKW] hormone 4e-07 [PIRKW] transmembrane protein 2e-06 [PIRKW] stomach 4e-07 [PIRKW] actm binding 7e-07 [PIRKW] ATP 7e-07 [PIRKW] phosphoprotem le-01 [PIRKW] signal transduction 7e-09 [PIRKW] heterotπmer 7e-09 [PIRKW] P-loop le-01 [PIRKW] hydrolase 7e-07 [PIRKW] transcription regulation 5e-06 [PIRKW] GTP binding 7e-09 [SUPFAM] l-phosphatιdylιnosιtol-4, 5-bisphosphate phosphodiesterase II 2e-07 [SUPFAM] SH3 homology 2e-07 [SUPFAM] SH2 homology 2e-07 [SUPFAM] protozoan myosin heavy chain IB 7e-07 [SUPFAM] myosin motor domain homology 7e-07 [SUPFAM] pleckstrm repeat homology 2e-07 [SUPFAM] protem-tyrosme kinase src 8e-06 [SUPFAM] WD repeat homology 3e-12 [SUPFAM] l-phosphatιdylιnosιtol-4, 5-bisphosphate phosphodiesterase domain Y homology 2e- 07 [SUPFAM] protein kinase homology 8e-06 [SUPFAM] l-phosphatιdylιnosιtol-4, 5-bisphosphate phosphodiesterase domain X homology 2e- 07 [SUPFAM] GTP-bindmg regulatory protein beta chain 7e-09 [SUPFAM] yeast coatomer complex alpha Cham 4e-07 [PROSITE] RGD 1 [PROSITE] MYRISTYL 6 [PROSITE] AMIDATION 2 [PROSITE] CAMP_PHOSPHO_SITE 4 [PROSITE] CK2_PHOSPHO_SITE 25 [PROSITE] TYR_PHOSPHO_SITE 4 [PROSITE] PKC_PHOSPHO_SITE 19 [PROSITE] ASN_GLYCOΞYLATION 6 [PFAM] Src homology domain 3 [PFAM] WD domain, G-beta repeats [KW] Irregular [KW] 3D [KW] LOW_COMPLEXITY 5.77 % [KW] COILED COIL 2.42 % SEQ MPTAESEAKVKTKVRFEKLLKTHSDLMREKKKLKKKLVRSEENIΞPDTIRSNLHYMKETT SEG xxxxxxxx COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCC IgotB SEQ SDDPDTIRSNLPHIKETTSDDVSAANTNNLKKSTRVTKNKLRNTQLATENPNGDASVEED SEG COILS IgotB SEQ KQGKPNKKVIKTVPQLTTQDLKPETPENKVDSTHQKTHTKPQPGVDHQKSEKANEGREET SEG XXX COILS IgotB SEQ DLEEDEELMQAYQCHVTEEMAKEIKRKIRKKLKEQLTYFPSDTLFHDDKLSSEKRKKKKE SEG XXXXXXXX XXXXXXXXXXXXXXX XXXXXXXXXXXX COILS IgotB SEQ VPVFSKAETSTLTISGDTVEGEQKKESSVRSVΞSDSHQDDEISSMEQSTEDSMQDDTKPK SEG XXXXXXXXXX xxxx COILS IgotB SEQ PKKTKKKTKAVADNNEDVDGDGVHEITSRDSPVYPKCLLDDDLVLGVYIHRTDRLKSDFM SEG xxxxxxxxx COILS IgotB SEQ ISHPMVKIHVVDEHTGQYVKKDDSGRPVSSYYEKENVDYILPIMTQPYDFKQLKSRLPEW SEG COILS IgotB SEQ EEQIVFNENFPYLLRGSDESPKVILFFEILDFLSVDEIKNNSEVQNQECGFRKIAWAFLK SEG COILS IgotB SEQ LLGANGNANINΞKLRLQLYYPPTKPRSPLSVVEAFEWWSKCPRNHYPSTLYVTVRGLKVP SEG COILS IgotB SEQ DCIKPSYRSMMALQEEKGKPVHCERHHESSSVDTEPGLEESKEVIKWKRLPGQACRIPNK SEG COILS IgotB SEQ HLFSLNAGERGCFCLDFSHNGRILAAACASRDGYPIILYEIPSGRFMRELCGHLNIIYDL SEG COILS IgotB CEEEEEECCCCCEEEE SEQ SWSKDDHYILTSSSDGTARIWKNEINNTNTFRVLPHPSFVYTAKFHPAVRELVVTGCYDS SEG COILS IgotB EETTTTTEEEEEETTTEEEEEETT—TTCEEEEEETTTCEEEEEETTT-TCEEEEEETTT SEQ MIRIWKVEMREDSAILVRQFDVHKSFINSLCFDTEGHHMYSGDCTGVIVVWNTYVKINDL SEG COILS IgotB EEEEEETTTTTBTTEEEEEEECCCCCE-EEEEEEETTEEEEEETTTEEEEEE SEQ EHΞVHHWTINKEIKETEFKGIPISYLEIHPNGKRLLIHTKDSTLRIMDLRILVARKFVGA SEG COILS IgotB SEQ ANYREKIHSTLTPCGTFLFAGSEDGIVYVWNPETGEQVAMYSDLPFKSPIRDISYHPFEN SEG COILS IgotB SEQ MVAFCAFGQNEPILLYIYDFHVAQQEAEMFKRYNGTFPLPGIHQSQDALCTCPKLPHQGS SEG COILS IgotB SEQ FQIDEFVHTESSSTKMQLVKQRLETVTEVIRSCAAKVNKNLSFTSPPAVSSQQSKLKQSN SEG COILS IgotB SEQ MLTAQEILHQFGFTQTGIISIERKPCNHQVDTAPTVVALYDYTANRSDELTIHRGDIIRV SEG COILS IgotB SEQ FFKDNEDWWYGSIGKGQEGYFPANHVASETLYQELPPEIKERSPPLSPEEKTKIEKSPAP SEG COILS IgotB SEQ QKQSINKNKSQDFRLGSESMTHSEMRKEQSHEDQGHIMDTRMRKNKQAGRKVTLIE SEG COILS IgotB Prosite for DKFZphtes3_ln3.1 PS00001 460->464 ASN_GLYCOSYLATION PDOC00001 PS00001 686->690 AΞN_GLYCOSYLATION PDOC00001 PS00001 934->938 ASN_GLYCOSYLATION PDOC00001 PS00001 1000->1004 ASN_GLYCOSYLATION PDOC00001 PS00001 1065->1069 ASN_GLYCOSYLATION PDOC00001 PS00001 1148->1152 ASN_GLYCOSYLATION PDOC00001 PS00004 91->95 CAMP_PHOSPHO_SITE PDOC00004 PS00004 264->268 CAMP_PHOSPHO_SITE PDOC00004 PS00004 305->309 CAMP_PHOSPHO_SITE PDOC00004 PS00004 1190->1194 CAMP_PHOSPHO_SITE PDOC00004 PS00005 48->51 PKC_PHOSPHO_SITE PDOC00005 PS00005 66->69 PKC_PHOSPHO_SITE PDOC00005 PS00005 93->96 PKC_PHOSPHO_SITE PDOC00005 PS00005 170->173 PKC_PHOSPHO_SITE PDOC00005 PS00005 232->235 PKC_PHOSPHO_SITE PDOC00005 PS00005 268->271 PKC_PHOSPHO_SITE PDOC00005 PS00005 304->307 PKC_PHOSPHO_SITE PDOC00005 PS00005 327->330 PKC_PHOSPHO_SITE PDOC00005 PS00005 352->355 PKC_PHOSPHO_SITE PDOC00005 PS00005 384->387 PKC_PHOSPHO_SITE PDOC00005 PS00005 440->443 PKC_PHOSPHO_SITE PDOC00005 PS00005 533->536 PKC_PHOSPHO_SITE PDOC00005 PS00005 546->549 PKC_PHOSPHO_SITE PDOC00005 PS00005 643->646 PKC_PHOSPHO_SITE PDOC00005 PS00005 677->680 PKC_PHOSPHO_SITE PDOC00005 PS00005 690->693 PKC_PHOSPHO_SITE PDOC00005 PS00005 702->705 PKC PHOSPHO SITE PDOC00005 PS00005 823->826 PKC PHOSPHO SITE PDOC00005 PS00005 973->976 PKC PHOSPHO " " SITE PDOC00005 PS00006 22->26 CK2 PHOSPHO " " SITE PDOC00006 PS00006 59->63 CK2 PHOSPHO " " SITE PDOC00006 PS00006 77->81 CK2 PHOSPHO " " SITE PDOC00006 PS00006 116->120 CK2 PHOSPHO " " SITE PDOC00006 PS00006 137->141 CK2 PHOSPHO " " SITE PDOC00006 PS00006 180->184 CK2 PHOSPHO " " SITE PDOC00006 PS00006 245->249 CK2 PHOSPHO " " SITE PDOC00006 PS00006 276->280 CK2 PHOSPHO " " SITE PDOC00006 PS00006 283->287 CK2 PHOSPHO " " SITE PDOC00006 PS00006 288->292 CK2 PHOSPHO " " SITE PDOC00006 PS00006 292->296 CK2 PHOSPHO " " SITE PDOC00006 PS00006 327->331 CK2 PHOSPHO " " SITE PDOC00006 PS00006 390->394 CK2 PHOSPHO " " SITE PDOC00006 PS00006 454->458 CK2 PHOSPHO " " SITE PDOC00006 PS00006 510->514 CK2 PHOSPHO " " SITE PDOC00006 PΞ00006 570->574 CK2 PHOSPHO " " SITE PDOC00006 PS00006 663->667 CK2 PHOSPHO " " SITE PDOC00006 PS00006 672->676 CK2 PHOSPHO " SITE PDOC00006 PS00006 804->808 CK2 PHOSPHO " " SITE PDOC00006 PS00006 985->989 CK2 PHOSPHO " SITE PDOC00006 PS00006 1023->1027 CK2 PHOSPHO " " SITE PDOC00006 PS00006 1127->1131 CK2 PHOSPHO " " SITE PDOC00006 PS00006 1132->1136 CK2 PHOSPHO " " SITE PDOC00006 PS00006 1161->1165 CK2 PHOSPHO " " SITE PDOC00006 PS00006 1170-M174 CK2 PHOSPHO " " SITE PDOC00006 PS00007 1083->1091 TYR PHOSPHO " SITE PDOC00007 PS00007 211->219 TYR PHOSPHO " SITE PDOC00007 PS00007 1083->1091 TYR PHOSPHO " " SITE PDOC00007 PS00007 210->219 TYR PHOSPHO " " SITE PDOC00007 PS00008 483->489 MYRISTYL PDOC00008 PS00008 577->583 MYRISTYL PDOC00008 PS00008 716->722 MYRISTYL PDOC00008 PS00008 800->806 MYRISTYL PDOC00008 PS00008 861->867 MYRISTYL PDOC00008 PS00008 941->947 MYRISTYL PDOC00008 PS00009 811->815 AMIDATION PDOC00009 PS00009 1188-M192 AMIDATION PDOC00009 PS00016 1074->1077 RGD PDOC00016 Pfam for DKFZphtes3_ln3.1 HMM_NAME WD domain, G-beta repeats HMM *MrGHnnWVWCVaFSPDGrWFIvSGSWDgTCRLWD* + GH+N ++++++S D ++ I+++S DGT R+W Query 650 LCGHLNIIYDLSWSKDDHY-ILTSSSDGTARIWK 682 HMM_NAME Src homology domain 3 HMM *pyVIALYDYqAqdpDELSFkEGDIIiIIEdsDD.WWrgRnnnTNGQEGW P+V+ALYDY+A+++DEL++ +GDII + ++++ WW+G GQEG+ Query 1054 PTVVALYDYTANRSDELTIHRGDIIRVFFKDNEDWWYGSIGK—GQEGY 1100 HMM IPSNYVEPi* +P+N V+ + Query 1101 FPANHVASE 1109 DKFZphtes3_20c21 group: testes derived DKFZphtes3_20c21 encodes a novel 708 ammo acid protein without similarity to known proteins. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application m studying the expression profile of testis-specific genes . unknown Sequenced by MediGenomix Locus: /map="22qll.2-12.2" Insert length: 3997 bp Poly A stretch at pos. 3877, polyadenylation signal at pos. 3853 1 GGTAGGCGGG GCGGCGCGTG ACCTAAGGCC TCTCTGCCGC GCGCGCAGGT 51 ACGGGGCAGA AGTCGCAGGT ACCCAGCTGC TGCCCACGTT TCTGGTCCAG 101 AGTCCCGAAC CCCGAGCACT GGGATGCCTG GCTACTCCGA GCCAAGGCAC 151 TGATGTTTGA ACTGGAAACT TCAAAACGTT TAATAAGAGT CTTCAGGATG 201 GGTTTGAACT AGACAAGCTA GAAATTTCTT TAGAACACCA GCTCTAGCAT 251 GCATCTCCCA CTTTTGGCTT TCCTGGAGAG GAGCTTGAAG AGGTGGTTCT 301 GCAGACAGCC ACAGTGATAC TCAGGAAACC AGAGGAATGG ATTTGACTTT 351 TCTGCTAGGA TTCTTTGTTA TAGTTTCTCC CTGAGTTGTA AGAGGCATGG 401 AAATATACAT GAAACTGAAG AACCTGCAAG GAAGGGAAGT GGAACTTTCC 451 ATGCTGAGTG AAAACTAACC AAGTGGCAGT TGTGACTGAA AACACTGAAA 501 CCTACCACGT CCAGATTCAC TGGATTGGGG GATAGAGGAA CGGTCACAGC 551 TAGGGAGAAA GAAGTGATAC CGGAAAAGAA AACCTAAATG AAGAGAATGA 601 GGATGACTGC ACAGTAGATG GCCACCTCTA CCTCCACAGA GGCAAAGTCA 651 GCCTCGTGGT GGAATTATTT TTTTCTTTAT GATGGTTCCA AGGTAAAGGA 701 AGAAGGCGAT CCAACAAGAG CTGGCATTTG TTACTTTTAT CCTTCCCAGA 751 CCCTGCTAGA CCAACAGGAG TTGCTTTGTG GACAGATTGC TGGAGTTGTC 801 CGCTGTGTTT CTGACATTTC TGACTCTCCT CCTACTCTTG TTCGTCTGAG 851 AAAACTGAAG TTTGCCATAA AAGTTGATGG AGATTACCTT TGGGTGCTGG 901 GCTGTGCTGT GGAGCTCCCT GATGTCAGCT GCAAGCGGTT TCTGGATCAG 951 CTAGTTGGAT TCTTTAATTT TTACAATGGA CCTGTTTCCC TAGCTTATGA 1001 GAACTGTTCT CAGGAAGAAC TGAGCACGGA GTGGGACACC TTCATCGAGC 1051 AAATTCTGAA AAACACCAGT GATCTGCATA AGATTTTCAA TTCCCTCTGG 1101 AACTTGGACC AAACTAAAGT GGAGCCCCTG TTGTTGCTGA AGGCAGCCCG 1151 CATTCTGCAG ACCTGCCAGC GCTCGCCTCA CATTCTCGCT GGCTGCATCC 1201 TCTATAAAGG ACTGATTGTC AGCACCCAAC TCCCGCCCTC CCTCACCGCC 1251 AAGGTCCTGC TTCACCGAAC AGCACCTCAG GAGCAGAGAC TCCCTACGGG 1301 AGGGGATGCC CCGCAGGAAC ATGGAGCGGC ATTGCCCCCG AATGTCCAGA 1351 TTATCCCTGT TTTTGTGACC AAAGAGGAAG CCATTAGTCT CCACGAGTTC 1401 CCGGTGGAAC AGATGACAAG GTCTCTAGCA TCTCCAGCAG GACTCCAGGA 1451 TGGTTCAGCC CAGCACCATC CAAAGGGTGG GAGCACATCT GCCCTGAAAG 1501 AAAACGCCAC TGGCCATGTG GAATCCATGG CCTGGACCAC CCCAGATCCC 1551 ACATCCCCTG ACGAAGCTTG TCCAGATGGC AGGAAGGAGA ACGGATGCTT 1601 GTCTGGCCAT GATCTGGAGA GCATCAGGCC CGCAGGACTG CACAACTCTG 1651 CCAGGGGTGA GGTTCTTGGC CTCAGCTCCT CCCTGGGGAA GGAACTAGTC 1701 TTTCTCCAAG AAGAACTCGA CTTGTCTGAA ATCCACATTC CAGAGGCTCA 1751 GGAAGTGGAA ATGGCCTCAG GTCATTTTGC CTTCCTACAT GTGCCTGTTC 1801 CAGATGGCAG GGCTCCTTAC TGCAAGGCAT CTCTCAGCGC CTCCAGCAGC 1851 CTGGAACCCA CGCCTCCTGA GGACACAGCC ATCAGCAGCT TGCGCCCTCC 1901 CTCTGCTCCT GAGATGCTGA CCCAGCATGG AGCCCAAGAG CAGGTCGAAG 1951 ACCATCCTGG CCATAGCAGC CAAGCCCCCA TTCCCAGAGC AGACCCTCTC 2001 CCCAGAAGGA CCCGCAGGCC CTTGTTATTG CCTCGCTTAG ATCCAGGACA 2051 GAGAGGAAAC AAGCTTCCCA CGGGGGAACA AGGCCTGGAT GAGGATGTTG 2101 ATGGGGTCTG TGAAAGCCAC GCAGCCCCTG GTCTGGAATG CAGTTCAGGC 2151 TCAGCAAACT GTCAGGGTGC TGGCCCCTCT GCAGATGGAA TCAGCTCCAG 2201 GCTGACACCA GCAGAGTCCT GCATGGGGCT CGTGAGGATG AATCTCTACA 2251 CTCACTGCGT CAAAGGGCTG ATGCTGTCCC TGCTGGCTGA GGAGCCGCTG 2301 CTGGGAGACA GCGCAGCCAT AGAGGAAGTG TACCACAGCA GCCTGGCTTC 2351 ACTGAATGGG CTGGAAGTCC ACCTGAAAGA GACGCTGCCC AGGGATGAGG 2401 CAGCCTCCAC GAGCAGCACC TACAACTTCA CATATTACGA CCGCATTCAG 2451 AGCTTGCTGA TGGCAAACCT GCCGCAGGTG GCCACCCCGC ATGATCGCCG 2501 CTTCCTCCAG GCCGTCAGCC TGATGCATAG CGAATTTGCC CAGCTGCCCG 2551 CGCTTTATGA AATGACTGTC AGAAATGCCT CCACGGCTGT GTACGCCTGT 2601 TGCAACCCCA TCCAGGAGAC ATATTTCCAG CAGCTGGCAC CTGCAGCACG 2651 GAGCTCCGGC TTCCCAAACC CTCAGGATGG CGCCTTCAGC CTCTCCGGCA 2701 AAGCAAAGCA GAAGCTGCTG AAGCACGGGG TGAACTTGCT CTGAACTGCA 2751 CCCAGGAGGT GACTGGGAAG GAGAAAACCA GCAAAGGAAG CTCTGCCTTT 2801 TATAATTGAA AAGGCCCCTC TATTTTATTT TTCTTGAAAA CATTCCCTTT 2851 TTTAGGAACC AAATGATATT TGAGTTTTTG TTATTCCTTT TGCAGATTGG 2901 GATGTGTTTT GGGGGCAGGG GTTAGTTCTT CAGGTCGGCA GACCCAGAGC 2951 ACTTGATAAA GAACTGTATT TAATCGGTAG TGTTGGGGCC GGGACGGGCT 3001 TGGCTCCCTC TCTGCCATAC TGAGCCTGAG GTATTTCATA TCTCCTGCTG 3051 TTCCATCCCA GCTTGAATTG GTGCCACAAG CTTCCAAGTT GGCATTTTTT 3101 CTAGAACCTG ATCGTCCACT AGCCCAGAGT GTGTGTGTTC AACCCCCACA 3151 CCAGGTGGTG GTAGGCGGTG TGACTGCACA GCGAGGTGCC GGATCTGTGA 3201 GCAGGCCGAC TCCACTCCCA CGCCGCAGGT AGGTTTCTCC AGTGCGCTCT 3251 TGCTGGGAGG TCCGGATCGT TCCTGCAGGG AAGCGGCAGC ACACGGAGAC 3301 CACTTGGTTG AATTCTGTTG GAACTCTACT CAAATCTAGG GGCGTCTTCT 3351 TTGGACCCAC AATGGGGGCA AGCCTTAATA ATATGGAAGG GAGTTTGGGC 3401 TTTAGAGATC CCTTTATAAA AGCTCTGGGG GCTGAGCCCT GAGAATTCAG 3451 TGACAACAGG ACCAACCTGC GCTGCCTTTG ACTACAAGTG GGCCGTGCAG 3501 CTGGTTCCTC TCGAGCGAGT GTCCCTAAAT AGGAGTTTAC AAGATGTCTG 3551 GGGGTAAAAG CACTGTGCTT TTCAGTGGTG GCTGCGTGAA AGGGAGCGAC 3601 ACTCAGCTGT GTGTTCCTGG GCTTGTGTGG TACTTAGAAC CTCAGTTCTA 3651 TTACGTTATA GTCAGACATT TTTTTGACAG TATGAGACAG ACTGCAGGAT 3701 GAAAATATTT GTCAAAATCT TAACTGAATG TTTACTGGAA GTACTTGAGA 3751 TTCCATTTGA GAGTTGTATT GTTAATAATT TCATGTCAGT GAACTGATAT 3801 CTGATGTTTA TGATATGGTG TCTTTTTCTT GAAACAAGCT TCCAAGGGCT 3851 AGAAATAAAA TAGCCAAAAA ATGCTGGAAA AAAAAAAAAA AAAAAAAAAA 3901 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 3951 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAA BLAST Results Entry HS1048E9 from database EMBLNEW: Human DNA sequence from clone 1048E9 on chromosome 22qll.2-12.2 Contains pseudogene similar to ribosomal protein S3A and part of a gene similar to C. elegans protein CE02118, ESTs, STS, GΞΞ. Score = 6540, P = O.Oe+00, identities = 1308/1308 -14 exons Medline entries No Medline entry Peptide information for frame 3 ORF from 618 bp to 2741 bp; peptide length: 708 Category: putative protein Classification: no clue 1 MATSTSTEAK SASWWNYFFL YDGSKVKEEG DPTRAGICYF YPSQTLLDQQ 51 ELLCGQIAGV VRCVSDISDS PPTLVRLRKL KFAIKVDGDY LWVLGCAVEL 101 PDVSCKRFLD QLVGFFNFYN GPVSLAYENC SQEELSTEWD TFIEQILKNT 151 SDLHKIFNSL WNLDQTKVEP LLLLKAARIL QTCQRSPHIL AGCILYKGLI 201 VSTQLPPSLT AKVLLHRTAP QEQRLPTGGD APQEHGAALP PNVQIIPVFV 251 TKEEAISLHE FPVEQMTRSL ASPAGLQDGS AQHHPKGGST ΞALKENATGH 301 VESMAWTTPD PTSPDEACPD GRKENGCLSG HDLESIRPAG LHNSARGEVL 351 GLSSSLGKEL VFLQEELDLS EIHIPEAQEV EMASGHFAFL HVPVPDGRAP 401 YCKASLSASS SLEPTPPEDT AISSLRPPSA PEMLTQHGAQ EQVEDHPGHS 451 SQAPIPRADP LPRRTRRPLL LPRLDPGQRG NKLPTGEQGL DEDVDGVCES 501 HAAPGLECSS GSANCQGAGP SADGISSRLT PAEΞCMGLVR MNLYTHCVKG 551 LMLSLLAEEP LLGDSAAIEE VYHSSLASLN GLEVHLKETL PRDEAASTSS 601 TYNFTYYDRI QSLLMANLPQ VATPHDRRFL QAVSLMHSEF AQLPALYEMT 651 VRNASTAVYA CCNPIQETYF QQLAPAARΞS GFPNPQDGAF SLSGKAKQKL 701 LKHGVNLL BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_20c21, frame 3 No Alert BLASTP hits found Pedant information for DKFZphtes3 20c21, frame 3 Report for DKFZphtes3 20c21.3 [ LENGTH ] 708 [MW] 76900 . 23 [pi] 5.30 [KW] Alpha Beta [KW] LOW COMPLEXITY 6 . 36 % SEQ MATSTSTEAKSASWWNYFFLYDGSKVKEEGDPTRAGICYFYPSQTLLDQQELLCGQIAGV SEG . xxxxxxxxxxxx PRD ccccccccccccccceeeeeccccccccccccccccceeeeccchhhhhhhhhhhcccee SEQ VRCVSDISDSPPTLVRLRKLKFAIKVDGDYLWVLGCAVELPDVSCKRFLDQLVGFFNFYN SEG PRD eeeeeeccccccchhhhhhhhheeeeccceeeeeeeeeecccccchhhhhhhhheeeecc SEQ GPVSLAYENCSQEELSTEWDTFIEQILKNTSDLHKIFNSLWNLDQTKVEPLLLLKAARIL SEG PRD ccccccccccchhhhhhhhhhhhhhhhhhcchhhhhhhcccccccccchhhhhhhhhhhh SEQ QTCQRSPHILAGCILYKGLIVSTQLPPSLTAKVLLHRTAPQEQRLPTGGDAPQEHGAALP SEG PRD hhhhccccchhhhhhhcccccccccccchhhhhhhhhccccccccccccccccccccccc SEQ PNVQIIPVFVTKEEAISLHEFPVEQMTRSLASPAGLQDGSAQHHPKGGSTSALKENATGH SEG PRD ccceeeeeeeecccceeeccccchhhhhhhccccccccccccccccccchhhhhhhcccc SEQ VESMAWTTPDPTSPDEACPDGRKENGCLSGHDLESIRPAGLHNSARGEVLGLSΞSLGKEL SEG PRD ccccccccccccccccccccccccccccccccccccccccccccccceeeeeccccchhh SEQ VFLQEELDLΞEIHIPEAQEVEMASGHFAFLHVPVPDGRAPYCKASLSASSSLEPTPPEDT SEG PRD hhhhhhhcccccccccchhhhhhccceeeeeecccccccceeeccccccccccccccccc SEQ AISSLRPPSAPEMLTQHGAQEQVEDHPGHSSQAPIPRADPLPRRTRRPLLLPRLDPGQRG SEG xxxxxxxxxxxxxxxxxxxxx .... PRD cccccccccchhhhhhccccceeecccccccccccccccccccccccccccccccccccc SEQ NKLPTGEQGLDEDVDGVCESHAAPGLECSSGSANCQGAGPSADGISSRLTPAESCMGLVR SEG PRD ccccccccccccccccccccccccccccccccccccccccccccccccccccccceeeee SEQ MNLYTHCVKGLMLSLLAEEPLLGDSAAIEEVYHSSLASLNGLEVHLKETLPRDEAASTSS SEG xxxxxxxxxxxx PRD ceeeeeeehhhhhhhhhccccccchhhhhhhhhhccccccchhhhhhhcccccccccccc SEQ TYNFTYYDRIQSLLMANLPQVATPHDRRFLQAVSLMHSEFAQLPALYEMTVRNAΞTAVYA SEG PRD ccceeeehhhhhhhhhcccccccccchhhhhhhhhhhhhhhcchhhhhhhhhccceeeee SEQ CCNPIQETYFQQLAPAARSSGFPNPQDGAFSLSGKAKQKLLKHGVNLL SEG PRD eccchhhhhhhhhhhhhhhcccccccccceeecchhhhhhhhhccccc (No Prosite data available for DKFZphtes3_20c21.3) (No Pfam data available for DKFZphtes3_20c21.3) DKFZphtes3_20k2 group: signal transduction DKFZphtes3_20k2 encodes a novel 839 amino acid protein with strong similarity to rat vamlloid receptor subtype 1. VRl seems to play an important role m the activation and sensitization of nociceptors. It is the receptor for e.g. capsaicin, a selective activator of nociceptors, a natural product of capsicum peppers. The novel protein is the human orthologue of rat VRl. The new protein can find application as a target for the development of new nociception- modulating drugs . strong similarity to rat vamlloid receptor subtype 1 Sequenced by MediGenomix Locus : unknown Insert length: 4187 bp Poly A stretch at pos. 4154, polyadenylation signal at pos. 4135 1 GGCTCAGGCA GGCCTGGCCC AGAGTCACGC TGGCAACCAC GAGTTTGGGA 51 AGCAGTCGTA TTCTCTCTCT CTCTCTCTCT CTCTCAGTAT CCATGACAGT 101 GTGATGGAGA GTCTCTGCCG TGCCATCTGG GATGCAAACC GTCCCTGTGT 151 CCCCCACGTC CAGGCCGTAG ATGCTCCCCG CCGGTCAGTC ACTTAGTCGT 201 CAGATCGCCC GTCCTGGTAT CACAGTGCTT CTGTTCAGGT TGCACACTGG 251 GCCACAGAGG ATCCAGCAAG GATGAAGAAA TGGAGCAGCA CAGACTTGGG 301 GGCAGCTGCG GACCCACTCC AAAAGGACAC CTGCCCAGAC CCCCTGGATG 351 GAGACCCTAA CTCCAGGCCA CCTCCAGCCA AGCCCCAGCT CTCCACGGCC 401 AAGAGCCGCA CCCGGCTCTT TGGGAAGGGT GACTCGGAGG AGGCTTTCCC 451 GGTGGATTGC CCTCACGAGG AAGGTGAGCT GGACTCCTGC CCGACCATCA 501 CAGTCAGCCC TGTTATCACC ATCCAGAGGC CAGGAGACGG CCCCACCGGT 551 GCCAGGCTGC TGTCCCAGGA CTCTGTCGCC GCCAGCACCG AGAAGACCCT 601 CAGGCTCTAT GATCGCAGGA GTATCTTTGA AGCCGTTGCT CAGAATAACT 651 GCCAGGATCT GGAGAGCCTG CTGCTCTTCC TGCAGAAGAG CAAGAAGCAC 701 CTCACAGACA ACGAGTTCAA AGACCCTGAG ACAGGGAAGA CCTGTCTGCT 751 GAAAGCCATG CTCAACCTGC ATGACGGACA GAACACCACC ATCCCCCTGC 801 TCCTGGAGAT CGCGCGGCAA ACGGACAGCC TGAAGGAGCT TGTCAACGCC 851 AGCTACACGG ACAGCTACTA CAAGGGCCAG ACAGCACTGC ACATCGCCAT 901 CGAGAGACGC AACATGGCCC TGGTGACCCT CCTGGTGGAG AACGGAGCAG 951 ACGTCCAGGC TGCGGCCCAT GGGGACTTCT TTAAGAAAAC CAAAGGGCGG 1001 CCTGGATTCT ACTTCGGTGA ACTGCCCCTG TCCCTGGCCG CGTGCACCAA 1051 CCAGCTGGGC ATCGTGAAGT TCCTGCTGCA GAACTCCTGG CAGACGGCCG 1101 ACATCAGCGC CAGGGACTCG GTGGGCAACA CGGTGCTGCA CGCCCTGGTG 1151 GAGGTGGCCG ACAACACGGC CGACAACACG AAGTTTGTGA CGAGCATGTA 1201 CAATGAGATT CTGATCCTGG GGGCCAAACT GCACCCGACG CTGAAGCTGG 1251 AGGAGCTCAC CAACAAGAAG GGAATGACGC CGCTGGCTCT GGCAGCTGGG 1301 ACCGGGAAGA TCGGGGTCTT GGCCTATATT CTCCAGCGGG AGATCCAGGA 1351 GCCCGAGTGC AGGCACCTGT CCAGGAAGTT CACCGAGTGG GCCTACGGGC 1401 CCGTGCACTC CTCGCTGTAC GACCTGTCCT GCATCGACAC CTGCGAGAAG 1451 AACTCGGTGC TGGAGGTGAT CGCCTACAGC AGCAGCGAGA CCCCTAATCG 1501 CCACGACATG CTCTTGGTGG AGCCGCTGAA CCGACTCCTG CAGGACAAGT 1551 GGGACAGATT CGTCAAGCGC ATCTTCTACT TCAACTTCCT GGTCTACTGC 1601 CTGTACATGA TCATCTTCAC CATGGCTGCC TACTACAGGC CCGTGGATGG 1651 CTTGCCTCCC TTTAAGATGG AAAAAATTGG AGACTATTTC CGAGTTACTG 1701 GAGAGATCCT GTCTGTGTTA GGAGGAGTCT ACTTCTTTTT CCGAGGGATT 1751 CAGTATTTCC TGCAGAGGCG GCCGTCGATG AAGACCCTGT TTGTGGACAG 1801 CTACAGTGAG ATGCTTTTCT TTCTGCAGTC ACTGTTCATG CTGGCCACCG 1851 TGGTGCTGTA CTTCAGCCAC CTCAAGGAGT ATGTGGCTTC CATGGTATTC 1901 TCCCTGGCCT TGGGCTGGAC CAACATGCTC TACTACACCC GCGGTTTCCA 1951 GCAGATGGGC ATCTATGCCG TCATGATAGA GAAGATGATC CTGAGAGACC 2001 TGTGCCGTTT CATGTTTGTC TACATCGTCT TCTTGTTCGG GTTTTCCACA 2051 GCGGTGGTGA CGCTGATTGA AGACGGGAAG AATGACTCCC TGCCGTCTGA 2101 GTCCACGTCG CACAGGTGGC GGGGGCCTGC CTGCAGGCCC CCCGATAGCT 2151 CCTACAACAG CCTGTACTCC ACCTGCCTGG AGCTGTTCAA GTTCACCATC 2201 GGCATGGGCG ACCTGGAGTT CACTGAGAAC TATGACTTCA AGGCTGTCTT 2251 CATCATCCTG CTGCTGGCCT ATGTAATTCT CACCTACATC CTCCTGCTCA 2301 ACATGCTCAT CGCCCTCATG GGTGAGACTG TCAACAAGAT CGCACAGGAG 2351 AGCAAGAACA TCTGGAAGCT GCAGAGAGCC ATCACCATCC TGGACACGGA 2401 GAAGAGCTTC CTTAAGTGCA TGAGGAAGGC CTTCCGCTCA GGCAAGCTGC 2451 TGCAGGTGGG GTACACACCT GATGGCAAGG ACGACTACCG GTGGTGCTTC 2501 AGGGTGGACG AGGTGAACTG GACCACCTGG AACACCAACG TGGGCATCAT 2551 CAACGAAGAC CCGGGCAACT GTGAGGGCGT CAAGCGCACC CTGAGCTTCT 2601 CCCTGCGGTC AAGCAGAGTT TCAGGCAGAC ACTGGAAGAA CTTTGCCCTG 2651 GTCCCCCTTT TAAGAGAGGC AAGTGCTCGA GATAGGCAGT CTGCTCAGCC 2701 CGAGGAAGTT TATCTGCGAC AGTTTTCAGG GTCTCTGAAG CCAGAGGACG 2751 CTGAGGTCTT CAAGAGTCCT GCCGCTTCCG GGGAGAAGTG AGGACGTCAC 2801 GCAGACAGCA CTGTCAACAC TGGGCCTTAG GAGACCCCGT TGCCACGGGG 2851 GGCTGCTGAG GGAACACCAG TGCTCTGTCA GCAGCCTGGC CTGGTCTGTG 2901 CCTGCCCAGC ATGTTCCCAA ATCTGTGCTG GACAAGCTGT GGGAAGCGTT 2951 CTTGGAAGCA TGGGGAGTGA TGTACATCCA ACCGTCACTG TCCCCAAGTG 3001 AATCTCCTAA CAGACTTTCA GGTTTTTACT CACTTTACTA AACAGTTTGG 3051 ATGGTCAGTC TCTACTGGGA CATGTTAGGC CCTTGTTTTC TTTGATTTTA 3101 TTCTTTTTTT TGAGACAGAA TTTCACTCTT CTCACCCAGG CTGGAATGCA 3151 GTGGCACAAT TTTGGCTCCC TGCAACCTCC GCCTCCTGGA TTCCAGCAAT 3201 TCTCCTGCCT CGGCTTCCCA AGTAGCTGGG ATTACAGGCA CGTGCCACCA 3251 TGTCTGGCTA ATTTTTTGTA TTTTTTTAAT AGATATGGGG TTTCGCCATG 3301 TTGGCCAGGC TGGTCTCGAA CTCCTGACCT CAGGTGATCC GCCCACCTCG 3351 GCCTCCCAAA GTGCTGGGAT TACAGGTGTG AGCCTCCACA CCTGGCTGTT 3401 TTCTTTGATT TTATTCTTTT TTTTTTTTCT GTGAGACAGA GTTTCACTCT 3451 TGTTGCCCAG GCTGGAGTGC AGTGGTGTGA TCTTGGCTCA CTGCAACCTC 3501 TGCCTCCCGG GTTCAAGCGA TTCTTCTGCT TCAGTCTCCC AAGTAGCTTG 3551 GATTACAGGT GAGCACTACC ACGCCCGGCT AATTTTTGTA TTTTTAATAG 3601 AGACGGGGTT TCACCATGTT GGCCAGGCTG GTCTCGAACT CTTGACCTCA 3651 GGTGATCTGC CCGCCTTGGC CTCCCAAAGT GCTGGGATTA CAGGTGTGAG 3701 CCGCTGCGCT CGGCCTTCTT TGATTTTATA TTATTAGGAG CAAAAGTAAA 3751 TGAAGCCCAG GAAAACACCT TTGGGAACAA ACTCTTCCTT TGATGGAAAA 3801 TGCAGAGGCC CTTCCTCTCT GTGCCGTGCT TGCTCCTCTT ACCTGCCCGG 3851 GTGGTTTGGG GGTGTTGGTG TTTCCTCCCT GGAGAAGATG GGGGAGGCTG 3901 TCCCACTCCC AGCTCTGGCA GAATCAAGCT GTTGCAGCAG TGCCTTCTTC 3951 ATCCTTCCTT ACGATCAATC ACAGTCTCCA GAAGATCAGC TCAATTGCTG 4001 TGCAGGTTAA AACTACAGAA CCACATCCCA AAGGTACCTG GTAAGAATGT 4051 TTGAAAGATC TTCCATTTCT AGGAACCCCA GTCCTGCTTC TCCGCAATGG 4101 CACATGCTTC CACTCCATCC ATACTGGCAT CCTCAAATAA ACAGATATGT 4151 ATACATATAA AAAAAAAAAA AAAAAAAAAA AAAAAAA BLAST Results No BLAST result Medline entries 99288727: Recent advances in neuropharmacology of cutaneous nociceptors. 99231880: A non-pungent triprenyl phenol of fungal origin, scutigeral, stimulates rat dorsal root ganglion neurons via interaction at vamlloid receptors . Peptide information for frame 2 ORF from 272 bp to 2788 bp; peptide length: 839 Category: strong similarity to known protein Classification: Cell signaling/communication 1 MKKWSSTDLG AAADPLQKDT CPDPLDGDPN SRPPPAKPQL STAKSRTRLF 51 GKGDSEEAFP VDCPHEEGEL DSCPTITVSP VITIQRPGDG PTGARLLSQD 101 SVAASTEKTL RLYDRRSIFE AVAQNNCQDL ESLLLFLQKS KKHLTDNEFK 151 DPETGKTCLL KAMLNLHDGQ NTTIPLLLEI ARQTDSLKEL VNASYTDSYY 201 KGQTALHIAI ERRNMALVTL LVENGADVQA AAHGDFFKKT KGRPGFYFGE 251 LPLSLAACTN QLGIVKFLLQ NSWQTADISA RDSVGNTVLH ALVEVADNTA 301 DNTKFVTSMY NEILILGAKL HPTLKLEELT NKKGMTPLAL AAGTGKIGVL 351 AYILQREIQE PECRHLSRKF TEWAYGPVHS SLYDLSCIDT CEKNΞVLEVI 401 AYSSSETPNR HDMLLVEPLN RLLQDKWDRF VKRIFYFNFL VYCLYMIIFT 451 MAAYYRPVDG LPPFKMEKIG DYFRVTGEIL SVLGGVYFFF RGIQYFLQRR 501 PΞMKTLFVDΞ YSEMLFFLQS LFMLATVVLY FSHLKEYVAS MVFSLALGWT 551 NMLYYTRGFQ QMGIYAVMIE KMILRDLCRF MFVYIVFLFG FSTAVVTLIE 601 DGKNDSLPSE STSHRWRGPA CRPPDSSYNS LYSTCLELFK FTIGMGDLEF 651 TENYDFKAVF IILLLAYVIL TYILLLNMLI ALMGETVNKI AQESKNIWKL 701 QRAITILDTE KSFLKCMRKA FRSGKLLQVG YTPDGKDDYR WCFRVDEVNW 751 TTWNTNVGII NEDPGNCEGV KRTLSFSLRΞ SRVSGRHWKN FALVPLLREA 801 SARDRQSAQP EEVYLRQFSG ΞLKPEDAEVF KSPAASGEK BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_20k2, frame 2 TREMBL:AF029310_1 product: "vamlloid receptor subtype 1"; Rattus norvegicus vamlloid receptor subtype 1 mRNA, complete eds., N = 1, Score = 3760, P = 0 TREMBLNEW:AB015231_1 product: "stretch-inhibitable nonselective channel (SIC)"; Rattus norvegicus mRNA for stretch-inhibitable nonselective channel (SIC), complete eds., N = 2, Score = 2090, P = 2e-219 >TREMBL:AF029310_1 product: "vamlloid receptor subtype 1"; Rattus norvegicus vamlloid receptor subtype 1 mRNA, complete eds. Length = 838 HSPs: Score = 3760 (564.1 bits), Expect = O.Oe+00, P = O.Oe+00 Identities = 721/839 (85%), Positives = 773/839 (92%) Query: 1 MKKWSSTDLGAAADPLQKDTCPDPLDGDPNSRPPPAKPQLSTAKSRTRLFGKGDSEEAFP 60 M++ +S D + P Q+++C DP D DPN +PPP KP + T +SRTRLFGKGDSEEA P Sbjct: 1 MEQRASLDSEESESPPQENSCLDPPDRDPNCKPPPVKPHIFTTRSRTRLFGKGDSEEASP 60 Query: 61 VDCPHEEGELDSCPTITVSPVITIQRPGDGPTGARLLSQDSVAASTEKTLRLYDRRSIFE 120 +DCP+EEG L SCP ITVS V+TIQRPGDGP R SQDSV+A EK RLYDRRSIF+ Sbjct: 61 LDCPYEEGGLASCPIITVSSVLTIQRPGDGPASVRPSSQDSVSAG-EKPPRLYDRRSIFD 119 Query: 121 AVAQNNCQDLESLLLFLQKΞKKHLTDNEFKDPETGKTCLLKAMLNLHDGQNTTIPLLLEI 180 AVAQ+NCQ+LESLL FLQ+SKK LTD+EFKDPETGKTCLLKAMLNLH+GQN TI LLL++ Sbjct: 120 AVAQSNCQELESLLPFLQRSKKRLTDSEFKDPETGKTCLLKAMLNLHNGQNDTIALLLDV 179 Query: 181 ARQTDSLKELVNASYTDSYYKGQTALHIAIERRNMALVTLLVENGADVQAAAHGDFFKKT 240 AR+TDSLK+ VNASYTDSYYKGQTALHIAIERRNM LVTLLVENGADVQAAA+GDFFKKT Sbjct: 180 ARKTDSLKQFVNASYTDSYYKGQTALHIAIERRNMTLVTLLVENGADVQAAANGDFFKKT 239 Query: 241 KGRPGFYFGELPLSLAACTNQLGIVKFLLQNSWQTADISARDSVGNTVLHALVEVADNTA 300 KGRPGFYFGELPLSLAACTNQL IVKFLLQNSWQ ADISARDSVGNTVLHALVEVADNT Sb j ct: 240 KGRPGFYFGELPLSLAACTNQLAIVKFLLQNSWQPADIΞARDSVGNTVLHALVEVADNTV 299 Query: 301 DNTKFVTSMYNEILILGAKLHPTLKLEELTNKKGMTPLALAAGTGKIGVLAYILQREIQE 360 DNTKFVTSMYNEILILGAKLHPTLKLEE+TN+KG+TPLALAA +GKIGVLAYILQREI E Sbjct: 300 DNTKFVTSMYNEILILGAKLHPTLKLEEITNRKGLTPLALAASSGKIGVLAYILQREIHE 359 Query: 361 PECRHLSRKFTEWAYGPVHSSLYDLSCIDTCEKNSVLEVIAYSSSETPNRHDMLLVEPLN 420 PECRHLSRKFTEWAYGPVHSSLYDLSCIDTCEKNSVLEVIAYSSSETPNRHDMLLVEPLN Sbjct: 360 PECRHLSRKFTEWAYGPVHSSLYDLSCIDTCEKNSVLEVIAYSSSETPNRHDMLLVEPLN 419 Query: 421 RLLQDKWDRFVKRIFYFNFLVYCLYMIIFTMAAYYRPVDGLPPFKMEK-IGDYFRVTGEI 479 RLLQDKWDRFVKRIFYFNF VYCLYMIIFT AAYYRPV+GLPP+K++ +GDYFRVTGEI Sbjct: 420 RLLQDKWDRFVKRIFYFNFFVYCLYMIIFTAAAYYRPVEGLPPYKLKNTVGDYFRVTGEI 479 Query: 480 LSVLGGVYFFFRGIQYFLQRRPSMKTLFVDSYSEMLFFLQSLFMLATVVLYFSHLKEYVA 539 LSV GGVYFFFRGIQYFLQRRPS+K+LFVDSYSE+LFF+QSLFML +VVLYFS KEYVA Sbjct: 480 LSVSGGVYFFFRGIQYFLQRRPSLKSLFVDSYΞEILFFVQSLFMLVSVVLYFSQRKEYVA 539 Query: 540 SMVFSLALGWTNMLYYTRGFQQMGIYAVMIEKMILRDLCRFMFVYIVFLFGFSTAVVTLI 599 SMVFSLA+GWTNMLYYTRGFQQMGIYAVMIEKMILRDLCRFMFVY+VFLFGFSTAVVTLI Sbjct: 540 SMVFSLAMGWTNMLYYTRGFQQMGIYAVMIEKMILRDLCRFMFVYLVFLFGFSTAVVTLI 599 Query: 600 EDGKNDSLPSESTSHRWRGPACRPPDSSYNSLYSTCLELFKFTIGMGDLEFTENYDFKAV 659 EDGKN+SLP EST H+ RG AC+P +SYNSLYSTCLELFKFTIGMGDLEFTENYDFKAV Sb j ct: 600 EDGKNNSLPMESTPHKCRGSACKP-GNSYNSLYSTCLELFKFTIGMGDLEFTENYDFKAV 658 Query: 660 FIILLLAYVILTYILLLNMLIALMGETVNKIAQESKNIWKLQRAITILDTEKSFLKCMRK 719 FIILLLAYVILTYILLLNMLIALMGETVNKIAQESKNIWKLQRAITILDTEKSFLKCMRK Sbjct: 659 FIILLLAYVILTYILLLNMLIALMGETVNKIAQESKNIWKLQRAITILDTEKSFLKCMRK 718 Query: 720 AFRSGKLLQVGYTPDGKDDYRWCFRVDEVNWTTWNTNVGIINEDPGNCEGVKRTLSFSLR 779 AFRSGKLLQVG+TPDGKDDYRWCFRVDEVNWTTWNTNVGIINEDPGNCEGVKRTLSFSLR Sb j ct: 719 AFRSGKLLQVGFTPDGKDDYRWCFRVDEVNWTTWNTNVGIINEDPGNCEGVKRTLSFSLR 778 Query: 780 SSRVSGRHWKNFALVPLLREASARDRQSAQPEEVYLRQFSGSLKPEDAEVFKSPAASGEK 839 S RVSGR+WKNFALVPLLR+AS RDR + Q EEV L+ ++GSLKPEDAEVFK GEK Sbjct: 779 SGRVSGRNWKNFALVPLLRDASTRDRHATQQEEVQLKHYTGSLKPEDAEVFKDSMVPGEK 838 Pedant information for DKFZphtes3_20k2, frame 2 Report for DKFZphtes3_20k2 .2 [LENGTH] 839 [MW] 94950 . 75 [pi ] 6 . 90 [HOMOL] TREMBL:AF029310_1 product: "vamlloid receptor subtype 1"; Rattus norvegicus vamlloid receptor subtype 1 mRNA, complete eds. 0.0 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YIL112w] 4e-05 [PIRKW] alternative splicing 3e-06 [PIRKW] peripheral membrane protein 3e-06 [SUPFAM] ankyrin repeat homology 3e-06 [SUPFAM] unassigned ankyrin repeat proteins 3e-06 [PFAM] Ank repeat [KW] TRANSMEMBRANE 4 SEQ MKKWSSTDLGAAADPLQKDTCPDPLDGDPNSRPPPAKPQLSTAKSRTRLFGKGDSEEAFP PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc MEM SEQ VDCPHEEGELDΞCPTITVSPVITIQRPGDGPTGARLLΞQDSVAASTEKTLRLYDRRSIFE PRD cccccccccccccccccceeeeeeecccccccceeeccccccccccchhhhhhhhhhhhh MEM SEQ AVAQNNCQDLESLLLFLQKSKKHLTDNEFKDPETGKTCLLKAMLNLHDGQNTTIPLLLEI PRD hhhhcchhhhhhhhhhhhhhcccccccccccccccchhhhhhhhhhccccccchhhhhhh MEM SEQ ARQTDSLKELVNASYTDSYYKGQTALHIAIERRNMALVTLLVENGADVQAAAHGDFFKKT PRD hhhcccccccccccccccccccchhhhhhhhhcchhhhhhhhhccceeeccccccccccc MEM SEQ KGRPGFYFGELPLSLAACTNQLGIVKFLLQNSWQTADISARDSVGNTVLHALVEVADNTA PRD ccccceeeccccchhhhhhcchhhhhhhhhcccccccccccccccchhhhhhhhhhcccc MEM SEQ DNTKFVTSMYNEILILGAKLHPTLKLEELTNKKGMTPLALAAGTGKIGVLAYILQREIQE PRD chhhhhhhhhhhhhhhccccccceeeeeecccccccchhhhhhhcchhhhhhhhhhhhhc MEM SEQ PECRHLSRKFTEWAYGPVHSSLYDLSCIDTCEKNSVLEVIAYSSSETPNRHDMLLVEPLN PRD ccccchhhhhheeeccceeeeeeeccccccccccccceeeeeccccccccceeeeehhhh MEM SEQ RLLQDKWDRFVKRIFYFNFLVYCLYMIIFTMAAYYRPVDGLPPFKMEKIGDYFRVTGEIL PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccccccccccccchhhhhhhhc MEM MMMMMMMMMMMMMMMMM SEQ SVLGGVYFFFRGIQYFLQRRPSMKTLFVDSYSEMLFFLQSLFMLATVVLYFSHLKEYVAS PRD cccceeeeeecchhhhhhhhheeeeeeccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhh MEM MMMMMMMMMMMMMMMMM SEQ MVFSLALGWTNMLYYTRGFQQMGIYAVMIEKMILRDLCRFMFVYIVFLFGFSTAVVTLIE PRD hhhhhhhhhhhhheeecccccccchhhhhhhhhhhhhhhhhhhheeecccccceeeeeec MEM MMMMMMMMMMMMMMMMM. SEQ DGKNDSLPSESTSHRWRGPACRPPDSSYNSLYSTCLELFKFTIGMGDLEFTENYDFKAVF PRD cccccccccccccccccccccccccccccchhhhhhhhhhhhhccccchhhhhhhhhhhh MEM MM SEQ IILLLAYVILTYILLLNMLIALMGETVNKIAQESKNIWKLQRAITILDTEKSFLKCMRKA PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh MEM MMMMMMMMMMMMMMM SEQ FRSGKLLQVGYTPDGKDDYRWCFRVDEVNWTTWNTNVGIINEDPGNCEGVKRTLSFSLRS PRD hhcceeeeeecccccccccceeeeeeecccccccccceeeecccccccceeeeeeeeeec MEM SEQ SRVSGRHWKNFALVPLLREASARDRQSAQPEEVYLRQFSGSLKPEDAEVFKSPAASGEK PRD ccccccccccchhhhhhhhhhhhhhhcccccceeeeecccccccccceeeecccccccc MEM (No Prosite data available for DKFZphtes3_20k2.2) Pfam for DKFZphtes3_20k2.2 HMM_NAME Ank repeat HMM *GyTPLHIAARyNNvEMVrlLLQHGADIN* G+T+LHIA +++N+ +V LL+++GAD+ Query 202 GQTALHIAIERRNMALVTLLVENGADVQ 229 DKFZphtes3_2013 group: transmembrane protein DKFZphtes3_2013 encodes a novel 595 amino acid protein with partial similarity to the IL-17 receptor. The novel protein contains one transmembrane region. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes and as a new marker for testicular cells. similarity to IL-17 receptor Sequenced by MediGenomix Locus : unknown Insert length: 2406 bp Poly A stretch at pos. 2345, no polyadenylation signal found 1 GCCTCAGGTG TTCCTGCGTT GTTTGTCAGT GGAGAGCAGG GAGTGGGGCC 51 AGCCAGCAGA AACAGTGGGC TGTACAACAT CACCTTCAAA TATGACAATT 101 GTACCACCTA CTTGAATCCA GTGGGGAAGC ATGTGATTGC TGACGCCCAG 151 AATATCACCA TCAGCCAGTA TGCTTGCCAT GACCAAGTGG CAGTCACCAT 201 TCTTTGGTCC CCAGGGGCCC TCGGCATCGA ATTCCTGAAA GGATTTCGGG 251 TAATACTGGA GGAGCTGAAG TCGGAGGGAA GACAGTGCCA ACAACTGATT 301 CTAAAGGATC CGAAGCAGCT CAACAGTAGC TTCAAAAGAA CTGGAATGGA 351 ATCTCAACCT TTCCTGAATA TGAAATTTGA AACGGATTAT TTCGTAAAGG 401 TTGTCCCTTT TCCTTCCATT AAAAACGAAA GCAATTACCA CCCTTTCTTC 451 TTTAGAACCC GAGCCTGTGA CCTGTTGTTA CAGCCGGACA ATCTAGCTTG 501 TAAACCCTTC TGGAAGCCTC GGAACCTGAA CATCAGCCAG CATGGCTCGG 551 ACATGCAGGT GTCCTTCGAC CACGCACCGC ACAACTTCGG CTTCCGTTTC 601 TTCTATCTTC ACTACAAGCT CAAGCACGAA GGACCTTTCA AGCGAAAGAC 651 CTGTAAGCAG GAGCAAACTA CAGAGATGAC CAGCTGCCTC CTTCAAAATG 701 TTTCTCCAGG GGATTATATA ATTGAGCTGG TGGATGACAC TAACACAACA 751 AGAAAAGTGA TGCATTATGC CTTAAAGCCA GTGCACTCCC CGTGGGCCGG 801 GCCCATCAGA GCCGTGGCCA TCACAGTGCC ACTGGTAGTC ATATCGGCAT 851 TCGCGACGCT CTTCACTGTG ATGTGCCGCA AGAAGCAACA AGAAAATATA 901 TATTCACATT TAGATGAAGA GAGCTCTGAG TCTTCCACAT ACACTGCAGC 951 ACTCCCAAGA GAGAGGCTCC GGCCGCGGCC GAAGGTCTTT CTCTGCTATT 1001 CCAGTAAAGA TGGCCAGAAT CACATGAATG TCGTCCAGTG TTTCGCCTAC 1051 TTCCTCCAGG ACTTCTGTGG CTGTGAGGTG GCTCTGGACC TGTGGGAAGA 1101 CTTCAGCCTC TGTAGAGAAG GGCAGAGAGA ATGGGTCATC CAGAAGATCC 1151 ACGAGTCCCA GTTCATCATT GTGGTTTGTT CCAAAGGTAT GAAGTACTTT 1201 GTGGACAAGA AGAACTACAA ACACAAAGGA GGTGGCCGAG GCTCGGGGAA 1251 AGGAGAGCTC TTCCTGGTGG CGGTGTCAGC CATTGCCGAA AAGCTCCGCC 1301 AGGCCAAGCA GAGTTCGTCC GCGGCGCTCA GCAAGTTTAT CGCCGTCTAC 1351 TTTGATTATT CCTGCGAGGG AGACGTCCCC GGTATCCTAG ACCTGAGTAC 1401 CAAGTACAGA CTCATGGACA ATCTTCCTCA GCTCTGTTCC CACCTGCACT 1451 CCCGAGACCA CGGCCTCCAG GAGCCGGGGC AGCACACGCG ACAGGGCAGC 1501 AGAAGGAACT ACTTCCGGAG CAAGTCAGGC CGGTCCCTAT ACGTCGCCAT 1551 TTGCAACATG CACCAGTTTA TTGACGAGGA GCCCGACTGG TTCGAAAAGC 1601 AGTTCGTTCC CTTCCATCCT CCTCCACTGC GCTACCGGGA GCCAGTCTTG 1651 GAGAAATTTG ATTCGGGCTT GGTTTTAAAT GATGTCATGT GCAAACCAGG 1701 GCCTGAGAGT GACTTCTGCC TAAAGGTAGA GGCGGCTGTT CTTGGGGCAA 1751 CCGGACCAGC CGACTCCCAG CACGAGAGTC AGCATGGGGG CCTGGACCAA 1801 GACGGGGAGG CCCGGCCTGC CCTTGACGGT AGCGCCGCCC TGCAACCCCT 1851 GCTGCACACG GTGAAAGCCG GCAGCCCCTC GGACATGCCG CGGGACTCAG 1901 GCATCTATGA CTCGTCTGTG CCCTCATCCG AGCTGTCTCT GCCACTGATG 1951 GAAGGACTCT CGACGGACCA GACAGAAACG TCTTCCCTGA CGGAGAGCGT 2001 GTCCTCCTCT TCAGGCCTGG GTGAGGAGGA ACCTCCTGCC CTTCCTTCCA 2051 AGCTCCTCTC TTCTGGGTCA TGCAAAGCAG ATCTTGGTTG CCGCAGCTAC 2101 ACTGATGAAC TCCACGCGGT CGCCCCTTTG TAACAAAACG AAAGAGTCTA 2151 AGCATTGCCA CTTTAGCTGC TGCCTCCCTC TGATTCCCCA GCTCATCTCC 2201 CTGGTTGCAT GGCCCACTTG GAGCTGAGGT CTCATACAAG GATATTTGGA 2251 GTGAAATGCT GGCCAGTACT TGTTCTCCCT TGCCCCAACC CTTTACCGGA 2301 TATCTTGACA AACTCTCCAA TTTTCTAAAA TGATATGGAG CTCTGAAAAA 2351 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 2401 AAAAAA BLAST Results o BLAST result Medline entries No Medline entry Peptide information for frame 1 ORF from 346 bp to 2130 bp; peptide length: 595 Category: similarity to known protein Classification: unclassified 1 MESQPFLNMK FETDYFVKVV PFPSIKNESN YHPFFFRTRA CDLLLQPDNL 51 ACKPFWKPRN LNISQHGSDM QVSFDHAPHN FGFRFFYLHY KLKHEGPFKR 101 KTCKQEQTTE MTSCLLQNVS PGDYIIELVD DTNTTRKVMH YALKPVHSPW 151 AGPIRAVAIT VPLVVISAFA TLFTVMCRKK QQENIYSHLD EESSESSTYT 201 AALPRERLRP RPKVFLCYSS KDGQNHMNVV QCFAYFLQDF CGCEVALDLW 251 EDFSLCREGQ REWVIQKIHE SQFIIVVCSK GMKYFVDKKN YKHKGGGRGS 301 GKGELFLVAV SAIAEKLRQA KQSSSAALSK FIAVYFDYSC EGDVPGILDL 351 STKYRLMDNL PQLCSHLHSR DHGLQEPGQH TRQGSRRNYF RSKSGRSLYV 401 AICNMHQFID EEPDWFEKQF VPFHPPPLRY REPVLEKFDS GLVLNDVMCK 451 PGPESDFCLK VEAAVLGATG PADSQHESQH GGLDQDGEAR PALDGSAALQ 501 PLLHTVKAGS PSDMPRDSGI YDSSVPSSEL SLPLMEGLST DQTETSSLTE 551 SVSSSSGLGE EEPPALPΞKL LSSGSCKADL GCRSYTDELH AVAPL BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_2013, frame 1 TREMBL :U58917_1 product: "IL-17 receptor"; Homo sapiens IL-17 receptor mRNA, complete eds., N = 1, Score = 215, P = 4.7e-14 TREMBL :MM31993_1 product: "interleukin 17 receptor"; Mus musculus interleukin 17 receptor mRNA, complete eds., N = 2, Score = 152, P = 1. le-13 >TREMBL:U58917_1 product: "IL-17 receptor"; Homo sapiens IL-17 receptor mRNA, complete eds. Length = 866 HSPs: Score = 215 (32.3 bits), Expect = 4.7e-14, P = 4.7e-14 Identities = 85/284 (29%), Positives = 131/284 (46%) Query: 213 KVFLCYSSKDGQNHMNVVQCFAYFLQDFCGCEVALDLWEDFSLCREGQREWV-IQK I 268 KV++ YS+ D +++VV FA FL CG EVALDL E+ ++ G WV QK + Sbjct: 379 KVWIIYSA-DHPLYVDVVLKFAQFLLTACGTEVALDLLEEQAISEAGVMTWVGRQKQEMV 437 Query: 269 HESQFIIVVCSKGMKY FVDKKNYXXXXXXXXXXXXELFLVAVSAIAEXXXXXXXXX 324 + IIV+CS+G + + + +LF A++ I Sbjct: 438 ESNSKIIVLCSRGTRAKWQALLGRGAPVRLRCDHGKPVGDLFTAAMNMILPDFKRPACFG 497 Query: 325 XXXXXXFIAVYF-DYSCEGDVPGILDLSTKYRLMDNLPQLCSHLHSRDHGLQEPGQHTRQ 383 ++ YF + SC+GDVP + + +Y LMD ++ + +D + +PG+ R Sbjct: 498 T YVVCYFSEVSCDGDVPDLFGAAPRYPLMDRFEEV--YFRIQDLEMFQPGRMHRV 550 Query: 384 G—SRRNYFRSKSGRSLYVAICNMHQFIDEEPDWFEKQFV PFHPPPLR YREPV 434 G S NY RS GR L A+ + PDWFE + + P L + EP+ Sbjct: 551 GELSGDNYLRSPGGRQLRAALDRFRDWQVRCPDWFECENLYSADDQDAPSLDEEVFEEPL 610 Query: 435 LEKFDSGLVLNDVMCKPGPESDFCLKVEAAVLGATGPADSQHESQHGGLDQDGEARP 491 L +G+V + + P S CL ++ V G G A H L G+ P Sb j ct: 611 LPP-GTGIVKRAPLVRE-PGSQACLAIDPLV-GEEGGAAVAKLEPH—LQPRGQPAP 662 Pedant information for DKFZphtes3_2013, frame 1 Report for DKFZphtes3_2013.1 [ LENGTH] 595 [MW] 66847 . 05 [pi ] 6 . 27 [ HOMOL] TREMBL : MM31993 1 product "interleukin 17 receptor " ; Mus musculus interleukin 17 receptor mRNA, complete eds . 2e- 14 [ BLOCKS ] BL00740A MAM domain proteins [ BLOCKS ] BL01224 B N-acetyl-gamma-glutamyl-phosphate reductase proteins [ KW] TRANSMEMBRANE 1 [ KW] LOW_COMPLEXITY 13 . 61 % SEQ MESQPFLNMKFETDYFVKVVPFPSIKNESNYHPFFFRTRACDLLLQPDNLACKPFWKPRN SEG PRD ccccccccccccccceeeeeccccccccccceeeeeeceeeeeeeccccccccccccccc MEM SEQ LNISQHGSDMQVSFDHAPHNFGFRFFYLHYKLKHEGPFKRKTCKQEQTTEMTSCLLQNVS SEG PRD eeeecccccceeeecccccccceeeeeehhhhhhcccchhhhhhhhhhhhhhhhhhcccc MEM SEQ PGDYIIELVDDTNTTRKVMHYALKPVHSPWAGPIRAVAITVPLVVISAFATLFTVMCRKK SEG PRD ccceeeeeeccccccccccccccccccccccccceeeeccchhhhhhhhhhhhhhhhhhh MEM MMMMMMMMMMMMMMMMM SEQ QQENIYSHLDEESSESSTYTAALPRERLRPRPKVFLCYΞSKDGQNHMNVVQCFAYFLQDF SEG xxxxxxx xxxxxxxxxx PRD hhhhhhhhhcccccccceeeeccccccccccceeeeeeecccccchhhhhhhhhhhhhhc MEM SEQ CGCEVALDLWEDFSLCREGQREWVIQKIHESQFIIVVCSKGMKYFVDKKNYKHKGGGRGS SEG xxxxxxxxx PRD ccchhhhhhhhccccccccchhhhhhhhhhheeeeeeeeccceeeeeccccccccccccc MEM SEQ GKGELFLVAVSAIAEKLRQAKQSSSAALSKFIAVYFDYSCEGDVPGILDLSTKYRLMDNL SEG XXX xxxxxxxxxxxxxx PRD ccceeeeehhhhhhhhhhhhhhcchhhhhhhheeeeccccccccccccccchhhhhhccc MEM SEQ PQLCSHLHSRDHGLQEPGQHTRQGSRRNYFRSKSGRSLYVAICNMHQFIDEEPDWFEKQF SEG PRD cchhhhhhcccccccccccccccccceeeeccccccceeeeeeceeeecccccceeeeee MEM SEQ VPFHPPPLRYREPVLEKFDSGLVLNDVMCKPGPESDFCLKVEAAVLGATGPADSQHESQH SEG PRD eecccccccccceeeeeccccceeeeecccccccccchhhhhhhhhhccccccccccccc MEM SEQ GGLDQDGEARPALDGSAALQPLLHTVKAGSPSDMPRDΞGIYDSSVPSSELΞLPLMEGLST SEG xxxxxxxxxxxxxxxxx . PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccchh MEM SEQ DQTETSSLTESVSSSSGLGEEEPPALPSKLLSSGSCKADLGCRSYTDELHAVAPL SEG .. xxxxxxxxxxxxxxxxxxxx PRD hhhhhhhhheeecccccccccccccccceeeccccceeeccccccccceeeeccc MEM (No Prosite data available for DKFZphtes3_2013.1) (No Pfam data available for DKFZphtes3_2013.1) DKFZphtes3_20ml8 group: nucleic acid management DKFZphtes3_20ml8 encodes a novel 132 amino acid protein with similarity to the S. cerevisiae mitochondrial carrier protein RIM2. The novel protein contains a leucine zipper and a Prosite mitochondrial energy transfer proteins signature. It is member of a family of substrate carrier proteins which are found in the inner mitochondrial membrane and are involved m energy transfer. The RIM2/MRS12 gene encodes a predicted protein of 377 ammo acids that is essential for mitochondrial DNA metabolism and proper cell growth. Inactivation of this gene causes the total loss of mitochondrial DNA and, compared to wild-type rhoo controls, a slow-growth phenotype on media containing glucose. The novel protein seems to be the human orthologue of this protein. The new protein can find application in modulation of mitochondrial DNA replication and maintenance. similarity to carrier protein RIM2 Sequenced by MediGenomix Locus : unknown Insert length: 3572 bp Poly A stretch at pos. 3530, polyadenylation signal at pos. 3510 1 GCCGCGGGGA GGGCTGTGCC GGTTGCTTTC TGCAGCCGCA TCTCGGCCAG 51 CTCTCCTCGC CGTCCCCGGG GCGCTGTGCG TCTCCAGTCC GGGACCGAAG 101 CCGCCTGCCG TAGCGGGCGG CCAGATCCGC GTCCCGCCTC AGCGGCCGGA 151 GGACATGCGG GAGAGAGAAT GAGCCAGAGG GACACGCTGG TGCATCTGTT 201 TGCCGGAGGA TGTGGTGGTA CAGTGGGAGC TATTCTGACA TGTCCACTGG 251 AAGTTGTAAA AACACGACTG CAGTCATCTT CTGTGACGCT TTATATTTCT 301 GAAGTTCAGC TGAACACCAT GGCTGGAGCC AGTGTCAACC GAGTAGTGTC 351 TCCCGGACCT CTTCATTGCC TAAAGGTGAT CTTGGAAAAA GAAGGGCCTC 401 GTTCCTTGTT TAGAGGACTA GGCCCCAATT TAGTGGGGGT AGCCCCTTCC 451 AGAGCAATAT ACTTTGCTGC TTATTCAAAC TGCAAGGAAA AGTTGAATGA 501 TGTATTTGAT CCTGATTCTA CCCAAGTACA TATGATTTCA GCTGCAATGG 551 CAGGTATGAA TGTATAATAT TAAAAAAAAA AAAAACTTTC TGAAACCTAG 601 AGGCTTAATA TTGAATTATA AGTTTGTAGT GAAAAGTTGA TGATTAATGT 651 GCTTTTCATT GATTAGATGA TTTTTACGTT TATCGATATA AACCAAATTA 701 GGTATATGTA AAATCTGTCA TCAGTTGACA TTTTTGTAGT CAGGAGTTTA 751 CATGCTAGGG TACAAGTAAT ATATTTATAT TGCCTTGTGT AGTCCACTGA 801 ATGTTTAGTG ATCATTGTTA ACAGTTTTAA GAATCCAACC ATAATTACAC 851 TATAAATAAG TTATGGAGCT GTAATTTACT CTTCTCTCCT CAATTTCTGT 901 TAGTGCCTTT TCCCTTTTTG CTGCATGTTT TGGCTTCTGT CTGAAATGTG 951 TCGGCAATTC TTGGTAAAGT ATTCATTTTG TCCTGTGCTC AAATGCTGAA 1001 ATTTTTGTGA GTGATGTATT ATTATTGACA ATTCAGTTAC TATGTGTATT 1051 TTTTAAAATT GTTTATTATT CTACATAATT CACACTAGAC AGCACCTGAA 1101 ATTTAGACAC TGGCTATGTG TACATGCTTA CTATAGAAAT GTTTCCAGGA 1151 ACTCTCTGTT TCTGTCATCA CTGATAAGTA TATATGATTC TGAATTAAAA 1201 TAACTAGTTT TAGGTCTTTA CCCTGCCATA AAGATAAACA GTTGGTTTGA 1251 CCAATCTGGT TCTGGAATCA TTTGCTGCTA TGCATGTTAG ACAAAGCCAC 1301 GAACTTTGAT TTTCCATTGA AAATTCTCCC TAATATCTGA GATTTATTGT 1351 ATATTTACTC ATATCTCACA TTTTCAAATT ATGCTGTAAC TTTATAAACT 1401 GTAGCTGCTT TCATCAGCTA TTGATCAATA AATTGAATGT CAATTATGTG 1451 CTTAATAATG AGTGCCTTAA ACTGTTAAAC ACTTTTGGTT TAGAAATAAA 1501 GTGAATCAAT TTGACCTATA TACTTCATGA AGTAAGTAAG TTTGAAATAC 1551 AAATTTCTGA AAGGTCAATA GCCCTTATCG TATTACAAAT TGTTTTTAAG 1601 GCTTTTTGTA TTTATTAATT GTCAGTTGAT TCACTGAAGC TTTAAAACTG 1651 GAAGGGACAA TCCAAAGGTC AAAAGAGTGA AATACAATCA TTTACCAATA 1701 AGGAAACCTT GGGCAAATTA TGTAATTTAT GTGAACCTCT CTTAGCTTAC 1751 CCATGGAATG AGTCAAGTGG TCTACATAGA TTTGGATTTT GAGAATTAGT 1801 TCTTTCATTT AGTGTTATAG AGATTATCTT GTTACAACTA GAATTATTTT 1851 TAATGTAATT TTTACAGATG TTGAATATTA GTAGATAGGA TTTTTCCCCT 1901 ACGAATTTGG ATGTAAGGTA AAGGTTGGTG GCCAGTGACA AACCTTATAA 1951 CCACTTTATC AGGTTCTTTA AAAATATATT TGTGAATTAC CAGTGATTAT 2001 GTTTTTGGCT TATAACCTCA GATAATTATA AAGAAATGTT AATCTTATTT 2051 GAAAGAATTG GAATCTAGAA AGTTAGATGA GCAGTCATTT TATATTGATA 2101 TTTGTTATAT CAGTATAGCA AATGCAGAGG TTCAGAATAT CTTTATTTCC 2151 ACTGGAACAT CTTATTTCAT TAGAGTATCT CATCAGAATT TATTACTGTA 2201 TTTGTATCAC ATTGCAAAGA ATTTCAGTAG AATTGTCAGT TTGCACTTTT 2251 TTCTCAAATG TGTACAAATG TTAACATATA GTTCATTTTT ATCTGTACAT 2301 TGATGCCATT TCCCAACTTG AATTCCTCAA GTTTTGGTAA ACTTACAATC 2351 TCATACTTGT TCAGAGGTTA TTGCACTGTA CACTTACTGT GTAGAAAATA 2401 CTGTTTGAAT TTGTTTGCAG TTACATTGTT CTGAGAACTG TGCTCTCAGA 2451 GCTTCTGTGC ACTATTCATG AGCATTAACA CTTAGCCTTG CAGTTTTATA 2501 CATAACTATA TGGTTAGTAA AACTGAATGG TCCAATGCAG ACTCATTAAA 2551 GTAGGCTTTT GCCCCCTTTG TTCTTGAAAT AATCTAGACC AGATTACTCG 2601 GGGTTTTTTT TAGGATTATT TTTATAGGTC TAAATATGAA TGATTTGGGG 2651 GTATGAAGTA CTTAAAGATA GTTCTGTGAA AAATCATTTT CAGCTGTCTA 2701 TTCAAGGGAA AAAATGCTAA CCTTGTCACT TTACTACACA AAACCACACT 2751 AAAATAAACC ATTAATGATA CTGCCTGCAA GATTTTAACA CACCAGATAG 2801 CACACACATT AAGGATTTAT AAGGCACTGT ACGTAATTTT TATTCCAAGT 2851 GACCTCTCAA TTCATTTTCA TTTTGCATTT TATCCATATG AACTCATGTT 2901 TAATTTAGAT AATAAAAATT TATTTTATTA AAAGGACAGT TTATTTAAAG 2951 TGGGTCTTTT TATTTGTTGT AGTGCATACT ATAAGAATTT GTAAGCCTCT 3001 AAAGTTGAGC TATAAATTTT CATGCATTAA AAATTTGTTT CAGTTGTGAG 3051 GATATTTAAT CAGATTAAAT AATGTTGACT CTTAATATTT TGCCTGCCTT 3101 TTTTTTCTCC TACACATGAC CTTTGACAGA CTAAGTATAT CTCAGCTATT 3151 GAGGGTATCT GTTTTGTTGC CTGTATATTT TGTTTAAATT AACTTGTATA 3201 TTCCTTTGTA TACACCTAGG CACAGATGTA TGCAAAAAAA ATTTGTTAAA 3251 TTACTTCTTT CTTTATACTA ATTCTCAATT TTTAAAAGAT TTTATCTGGC 3301 ATGTATATAC TTTTATATAG AACATTATAA ATGTAAAGGA AATGAATTCT 3351 AATTTTAATT GGATTATGTA TTCATACAGT TATTCTCAAT TTTTAAAATA 3401 CTAATAATGT AATCATTGAA TGTTTCCTAC ATACGTAGTG GGTTTTATTT 3451 GCTCACAGCA TACAGTTATT TTTCAATTTA TGTTTTTCTA TTAGACTTAA 3501 ATTTCATTAT AATAAAGGCT TTTACTCATT AAATACAAAA AAAAAAAAAA 3551 AAAAAAAAAA AAAAAAAAAA AA BLAST Results No BLAST result Medlme entries No Medl e entry 95198680: Overexpression of a novel member of the mitochondrial carrier family rescues defects m both DNA and RNA metabolism m yeast mitochondria. Peptide information for frame 1 ORF from 169 bp to 564 bp; peptide length: 132 Category: similarity to known protein Classification: Intacellular transport and traffic Prosite motifs: LEUCINE_ZIPPER (27-49) MITOCH CARRIER (26-36) 1 MSQRDTLVHL FAGGCGGTVG AILTCPLEVV KTRLQSSSVT LYISEVQLNT 51 MAGASVNRVV SPGPLHCLKV ILEKEGPRSL FRGLGPNLVG VAPSRAIYFA 101 AYSNCKEKLN DVFDPDSTQV HMISAAMAGM NV BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_20ml8, frame 1 PIR:Ξ44092 probable carrier protein c2 - Caenorhabditis elegans, N = 2, Score = 147, P = 1.5e-19 PIR:S36081 probable carrier protein RIM2, mitochondrial - yeast (Saccharomyces cerevisiae), N = 1, Score = 230, P = 6.2e-19 >PIR:S36081 probable carrier protein RIM2, mitochondrial - yeast (Saccharomyces cerevisiae) Length = 377 HSPs: Score = 230 (34.5 bits), Expect = 6.2e-19, P = 6.2e-19 Identities = 55/133 (41%), Positives = 80/133 (60%) Query: 8 VHLFAGGCGGTVGAILTCPLEVVKTRLQSSS-VTLYISEVQLNTMAGA SVNRVVSP 62 VH AGG GG GA++TCP ++VKTRLQS + Y S+ +N G+ S+N V+ Sbjct: 54 VHFVAGGIGGMAGAVVTCPFDLVKTRLQSDIFLKAYKSQA-VNISKGSTRPKSINYVIQA 112 Query: 63 GP LHCLKVILEKEGPRSLFRGLGPNLVGVAPSRAIYFAAYSNCKEKLNDVFD—P 115 G L + + ++EG RSLF+GLGPNLVGV P+R+I F Y K+ F+ Sbjct: 113 GTHFKETLGIIGNVYKQEGFRSLFKGLGPNLVGVIPARSINFFTYGTTKDMYAKAFNNGQ 172 Query: 116 DSTQVHMIΞAAMAG 129 ++ +H+++AA AG Sbjct: 173 ETPMIHLMAAATAG 186 Score = 77 (11.6 bits), Expect = l.le+00, P = 6.8e-01 Identities = 25/88 (28%), Positives = 39/88 (44%) Query: 3 QRDTLVHLFAGGCGGTVGAILTCPLEVVKTRLQSSΞVTLYISEVQLNTMAGASVNRVVSP 62 Q ++HL A G A T P+ ++KTR VQL+ SV + + Sbjct: 172 QETPMIHLMAAATAGWATATATNPIWLIKTR VQLDKAGKTSVRQYKNS 2 IS Query: 63 GPLHCLKVILEKEGPRSLFRGLGPNLVG 90 CLK ++ EG L++GL + +G Sbjct: 220 WD--CLKSVIRNEGFTGLYKGLSASYLG 245 Score = 71 (10.7 bits), Expect = 6.6e+00, P = l.Oe+00 Identi .ties : = 28/91 (30%), Positives = 45/91 (49%) Query: 12 AGGCGGTVGAILTCPLEVVKTRLQSSSVTLYISEVQLNTMAGASVNRVVSPGPLHCLKVI 71 + G V +1 T P EVV+TRL+ + + N G R + G + KVI Sbjct: 294 SAGLAKFVASIATYPHEVVRTRLRQTP KEN G KRKYT-GLVQΞFKVI 338 Query: 72 LEKEGPRSLFRGLGPNLVGVAPSRAIYFAAY 102 +++EG S++ GL P+L+ P+ I F + Sbjct: 339 IKEEGLFSMYSGLTPHLMRTVPNSIIMFGTW 369 Pedant information for DKFZphtes3_20ml8, frame 1 Report for DKFZphtes3_20ml8.1 [LENGTH) 132 [MW] 13993 .36 [pl] 8.42 [HOMOL] PIR:S 36081 probable carrier protein RIM2, mitochondrial - yeast (Saccharomyces cerevisiae) 7e-19 [ FUNCAT ] 07 . 16 purine and pyπmidme transporters [S. cerevisiae, YBR192w] 3e-20 [FUNCAT] 08 . 04 mitochondrial transport [S. cerevisiae, YBR192w] 3e-20 [ FUNCAT] 30 . 16 mitochondrial organization [S. cerevisiae, YBR192w] 3e-20 [FUNCAT] 02 . 13 respiration [S. cerevisiae, YBR192w] 3e-20 [FUNCAT] 01 . 05 .07 carbohydrate transport [S. cerevisiae, YPR021c] 3e-10 [FUNCAT] 07 . 07 sugar and carbohydrate transporters [S. cerevisiae, YPR021c] 3e-10 [FUNCAT] 07 . 99 other transport facilitators [S. cerevisiae, YEL006w] le-09 [FUNCAT] 01 . 07 .10 transport of vitamins, cofactors, and prosthetic groups [S. cerevisiae, YIL006w] 3e-09 [ FUNCAT] 07.04 .07 amon transporters (cl, so4, po4, etc.) [S. cerevisiae, YKL120w] 2e-08 [FUNCAT] 01.03.19 nucleotide transport [S. cerevisiae, YPROllc] 3e-08 [FUNCAT] 04.05.03 mrna processing (splicing) [S. cerevisiae, YKR052c] 4e-08 [FUNCAT] 01.05.04 regulation of carbohydrate utilization [S. cerevisiae, YJR095w] 2e-07 [FUNCAT] 01.01.07 ammo-acid transport [S. cerevisiae, YOR130c] 5e-05 [FUNCAT] 07.10 amino-acid transporters [S. cerevisiae, YOR130c] 5e-05 [FUNCAT] 01.04.07 phosphate transport [S. cerevisiae, YJR077c] 7e-05 [FUNCAT] 13.04 homeostasis of other ions [S. cerevisiae, YJR077c] 7e-05 [BLOCKS] BL00215B Mitochondrial energy transfer proteins [BLOCKS] BL00215A Mitochondrial energy transfer proteins [PIRKW] duplication 6e-09 [PIRKW] transmembrane protein 6e-09 [PIRKW] mitochondrial inner membrane 4e-07 [PIRKW] transport protein 5e-06 [PIRKW] mitochondrion 7e-08 [PIRKW] chloroplast 3e-08 [SUPFAM] Btl protein 3e-08 [SUPFAM] ADP, ATP carrier protein repeat homology 4e-09 [SUPFAM] Caenorhabditis probable carrier protein c2 4e-09 [SUPFAM] probable carrier protein YPR021c 6e-09 [PROSITE] LEUCINE_ZIPPER 1 [PROSITE] MITOCH_CARRIER 1 [PFAM] Mitochondrial carrier proteins [KW] Alpha_Beta SEQ MSQRDTLVHLFAGGCGGTVGAILTCPLEVVKTRLQSSSVTLYISEVQLNTMAGASVNRVV PRD cccccceeeecccccccceeeeeecchhhhhhhhhhhccccccccccccccccccccccc SEQ SPGPLHCLKVILEKEGPRSLFRGLGPNLVGVAPSRAIYFAAYSNCKEKLNDVFDPDSTQV PRD cccchhhhhhhhhhcccceeeeccccceeeecccceeeeeehhhhhhhhhcccccccccc SEQ HMISAAMAGMNV PRD chhhhhhhcccc Prosite for DKFZphtes3_20ml8.1 PS00029 27->49 LEUCINE_ZIPPER PDOC00029 PS00215 26->36 MITOCH CARRIER PDOC00189 Pfam for DKFZphtes3_20ml8.1 HMM_NAME Mitochondrial carrier proteins HMM *pFwkdFLAGGIAGmMeHTvMFPIDtIKTRMQlQgEMpM.. ahpR +++++++AGG +G + +++++P++++KTR+Q++ ++ + ++ Query 5 DTLVHLFAGGCGGTVGAILTCPLEVVKTRLQSS-SVTLYISEVQLNTMA 52 HMM YkGMIdCFRwIwkNEGWRGLWRGLgANvIRYIPqWalRFGFY G+++C++ I+++EG+R+L+RGLG+N+++++P +AI+F+ Y Query 53 GASVNRVVSPGPLHCLKVILEKEGPRSLFRGLGPNLVGVAPSRAIYFAAY 102 HMM EFMKeMFiDyfgeddnyWmWFwmnYMaGs* +KE ++D F++ D++++++ + +MAG+ Query 103 SNCKEKLNDVFDP-DSTQVHMISAAMAGM 130 DKFZphtes3_21d4 group: signal transduction DKFZphtes3_21d4 encodes a novel 464 ammo acid putative GTP exchanging factor related to RCCl. RCCl (regulator of chromosome condensation) is a eukaryotic protein which binds to chromatin and interacts with ran, a nuclear GTP-binding protein. RCCl promotes the exchange of bound GDP with GTP, acting as a guamne-nucleotide dissociation stimulator. The new protein can find application in the regulation of gene expression by activition of nuclear GTP-binding proteins. The X-linked retinitis pigmentosa is a result of a defect GTPase regulator, which contains a RCCl-type repeat. similarity to RCCl-like G exchanging factor RLG complete cDNA, complete eds, EST hits Sequenced by LMU Locus: /map="20" Insert length: 2321 bp Poly A stretch at pos. 2293, polyadenylation signal at pos. 2262 1 GGGTCACGCA AGATGGCGGC GCCCAGAGGC TGCTGAGGCG CGGAACGGAG 51 GATGGCGCTG GTGGCGTTGG TGGCTGGGGC TCGGCTGGGG CGGCGGCTGA 101 GCGGGCCGGG GCTGGGGCGA GGGCACTGGA CGGCGGCCAG GCGCTCCCGG 151 AGCCGGCGCG AAGCGGCAGA AGCCGAGGCG GAGGTGCCCG TGGTCCAGTA 201 CGTGGGCGAG CGCGCTGCCC GCGCCGATCG CGTCTTCGTG TGGGGCTTCA 251 GCTTCTCGGG GGCGCTGGGC GTGCCTTCCT TTGTGGTGCC CAGCTCCGGG 301 CCCGGGCCCC GCGCCGGCGC CCGACCGCGC CGCAGGATCC AGCCCGTGCC 351 CTATCGCCTG GAGCTGGACC AAAAGATTTC ATCTGCTGCT TGCGGCTATG 401 GATTCACACT GCTGTCCTCT AAGACTGCGG ATGTTACGAA AGTCTGGGGG 451 ATGGGACTCA ACAAAGATTC TCAGCTTGGA TTTCACAGGA GCCGGAAAGA 501 TAAAACGAGG GGCTACGAGT ATGTGTTGGA GCCCTCACCC GTCTCCCTGC 551 CTCTGGACAG ACCTCAGGAG ACACGGGTGC TGCAGGTCTC CTGCGGCCGA 601 GCTCACTCTC TTGTGTTGAC TGACAGGGAA GGAGTCTTCA GCATGGGAAA 651 CAATTCTTAT GGGCAATGTG GAAGAAAGGT GGTCGAAAAT GAAATTTACA 701 GTGAAAGTCA CAGAGTCCAC AGGATGCAGG ACTTCGATGG CCAGGTGGTC 751 CAGGTCGCCT GTGGTCAGGA TCATAGTCTG TTCCTGACGG ATAAAGGAGA 801 AGTCTATTCT TGTGGATGGG GTGCTGATGG GCAAACAGGT CTGGGTCACT 851 ACAATATCAC CAGCTCGCCC ACCAAGCTGG GTGGAGACCT GGCGGGAGTG 901 AACGTTATCC AAGTTGCCAC CTACGGTGAT TGCTGCCTGG CCGTGTCCGC 951 CGACGGAGGA CTTTTTGGTT GGGGAAACTC GGAGTACCTG CAGCTGGCCT 1001 CTGTCACTGA CTCCACACAG GTGAATGTGC CCCGCTGCTT ACACTTCTCA 1051 GGAGTGGGGA AGGTGCGACA GGCTGCATGC GGTGGCACGG GCTGTGCAGT 1101 GTTAAACGGA GAAGGACATG TTTTTGTCTG GGGCTATGGA ATTCTTGGGA 1151 AAGGTCCAAA CCTAGTGGAA AGTGCCGTCC CTGAAATGAT TCCACCCACT 1201 CTCTTTGGCT TGACGGAGTT CAACCCAGAA ATCCAGGTTT CCCGCATCCG 1251 ATGTGGACTC AGCCACTTTG CTGCACTGAC CAACAAAGGA GAGCTGTTTG 1301 TATGGGGCAA GAACATCCGA GGGTGCCTGG GAATCGGTCG CCTGGAGGAC 1351 CAGTATTTCC CATGGAGGGT GACGATGCCT GGGGAGCCTG TGGACGTGGC 1401 ATGTGGCGTG GACCACATGG TGACCCTGGC CAAGTCATTC ATCTAAACCT 1451 CCCTCACCTG CTTGGGCGGC CCCGTCCCGG GAACCACTGG CACTCCTTGG 1501 CAGAGGCCAG CGCGTGGCCA GCCCCCCGGG GTTCTTGGAT GGTGGTGGCG 1551 GAGGACCCTG CGTGCAGTGT GACGCTCTGT CCTGAATCCC TTAGCGGGTA 1601 CCTACCAGGA GGATCAGGGC AAGGTCCCTC TCCAGCTGCA GGTGAGGCCT 1651 GCGGAACTCA GCTTGGATGG CAGCCTTTGG TGGGCCGCTG TGGCCCGCAC 1701 GTCTCTGTTC TCTCCAAGTA ACATGCGACG GTGTCTGGTG TCACGTCTCG 1751 CCTGAGAAGC CCGTCTTAGG AAAGCTTAGC TTGAACACAG TGCTCGGGAG 1801 GTTTCTGCTC TGTCTGTCAT GGCAGTCTCT TGGTTTGTGT CTGGCCAAGG 1851 CCATGCGTGT GCCTCGGACC GAGCCCCAGC TTAGGCGAGG GAGTCAGGCT 1901 GGCTTCGGCC CTCGGTTTTC ATTCAGGCCA CCCTGCTCAT GGCCCTTCCT 1951 GGCCGCCTGC CACACCGCAA GCTCGCTGGG GGGACACTAG AAGCACCGTG 2001 GCCTGGGATT CCATCTGGAG CTGTCCGCAG GCACCAGCCC CAGCCTCCCA 2051 CCACGCTCAC TGCCTGGCTT GGAAAAGTTA AGAAGCCCCT CAGGAAGAGA 2101 ATCGAGGCTA AGTTCCTCTG CGCCGAGGGC CCCGAGCATA TCCGCCAAGG 2151 CTCAGCTGCA GTGCCAGGCG GAGGAGGAAG ATCCAGAAAT TGTGAACAAT 2201 GTTTGATTTA GTAGCGTGAC TTGCCTTTCC CTTTAAAAAC ATCTTTTACA 2251 AATCTGTCTT GGAATAAAGT CTATTTTCTG CCTTTTGGTT TTTAAAAAAA 2301 AAAAAAAAAA AAAAAAAAAA A BLAST Results Entry HS203358 from database EMBL: human STS SHGC-31781. Score = 1748, P = l.le-72, identities = 376/394 Medline entries No Medline entry Peptide information for frame 1 ORF from 52 bp to 1443 bp; peptide length: 464 Category: similarity to known protein 1 MALVALVAGA RLGRRLSGPG LGRGHWTAAR RSRSRREAAE AEAEVPVVQY 51 VGERAARADR VFVWGFSFSG ALGVPSF VP SSGPGPRAGA RPRRRIQPVP 101 YRLELDQKIS SAACGYGFTL LSSKTADVTK VWGMGLNKDS QLGFHRSRKD 151 KTRGYEYVLE PSPVSLPLDR PQETRVLQVS CGRAHSLVLT DREGVFSMGN 201 NSYGQCGRKV VENEIYSEΞH RVHRMQDFDG QVVQVACGQD HΞLFLTDKGE 251 VYΞCGWGADG QTGLGHYNIT SSPTKLGGDL AGVNVIQVAT YGDCCLAVSA 301 DGGLFGWGNS EYLQLASVTD STQVNVPRCL HFSGVGKVRQ AACGGTGCAV 351 LNGEGHVFVW GYGILGKGPN LVESAVPEMI PPTLFGLTEF NPEIQVSRIR 401 CGLSHFAALT NKGELFVWGK NIRGCLGIGR LEDQYFPWRV TMPGEPVDVA 451 CGVDHMVTLA KSFI BLASTP hits Entry CEW09G3_5 from database TREMBLNEW: gene: "W09G3.3"; Caenorhabditis elegans cosmid W09G3 Score = 395, P = 9.3e-37, identities = 111/330, positives = 165/330 Entry Y032_HUMAN from database SWISSPROT: HYPOTHETICAL PROTEIN KIAA0032. Score = 309, P = 1.0e-24, identities = 96/308, positives = 143/308 Entry B38919 from database PIR: hypothetical protein 2 - human (fragment) Score = 309, P = 1.0e-24, identities = 96/308, positives = 143/308 Entry AF060219_1 from database TREMBLNEW: product: "RCCl-like G exchanging factor RLG"; Homo sapiens RCCl-like G exchanging factor RLG mRNA, complete eds. Score = 273, P = 4.0e-21, identities = 84/262, positives = 124/262 Entry S71752 from database PIR: giant protein p619 - human Score = 282, P = 1. le-19, identities = 86/287, positives = 144/287 Alert BLASTP hits for DKFZphtes3_21d4, frame 1 No Alert BLASTP hits found Pedant information for DKFZphtes3_21d4, frame 1 Report for DKFZphtes3_21d4.1 [LENGTH] 464 [MW] 49997.08 [pi] 8.74 [HOMOL] TREMBL :CEW09G3_5 gene: "W09G3.3"; Caenorhabditis elegans cosmid W09G3 5e-34 [FUNCAT] 04.07 rna transport [S. cerevisiae, YGL097w] 2e-09 [FUNCAT] 03.07 pheromone response, matmg-type determination, sex-specific proteins [S. cerevisiae, YGL097w] 2e-09 [FUNCAT] 08.01 nuclear transport [S. cerevisiae, YGL097w] 2e-09 [FUNCAT] 04.05.05 mrna processing (5'-end, 3'-end processing and mrna degradation) [S. cerevisiae, YGL097w] 2e-09 [FUNCAT] 04.01.04 rrna processing [S. cerevisiae, YGL097w] 2e-09 [FUNCAT] 04.03.03 trna processing [S. cerevisiae, YGL097w] 2e-09 [FUNCAT] 30.03 organization of cytoplasm [Ξ. cerevisiae, YGL097w] 2e-09 [FUNCAT] 30.04 organization of cytoskeleton [Ξ. cerevisiae, YAL020c] 4e-06 [BLOCKS] BL00870I [BLOCKS] BL00625B Regulator of chromosome condensation (RCCl) proteins [BLOCKS] BL00625A Regulator of chromosome condensation (RCCl) proteins [PIRKW] blocked ammo end 3e-16 [PIRKW] nucleus 3e-16 [PIRKW] duplication 4e-08 [PIRKW] tandem repeat 3e-16 [PIRKW] DNA binding 3e-16 [PIRKW] mitosis 3e-16 [PIRKW] leucine zipper 3e-21 [SUPFAM] pheromone response pathway component SRM1 4e-08 [SUPFAM] WD repeat homology 3e-21 [PROSITE] MYRISTYL 7 [PROSITE] RCC1_2 2 [PROSITE] AMIDATION 2 [PROSITE] CAMP_PHOSPHO_SITE 1 [PROSITE] CK2_PHOSPHO_SITE 5 [PROSITE] TYR_PHOSPHO_SITE 2 [PROSITE] GLYCOSAMINOGLYCAN 3 [PROSITE] PKC_PHOSPHO_SITE 7 [PROSITE] ASN_GLYCOSYLATION 2 [PFAM] Regulator of chromosome condensation (RCCl) [KW] All_Beta [KW] LOW COMPLEXITY 13.58 % SEQ MALVALVAGARLGRRLSGPGLGRGHWTAARRSRSRREAAEAEAEVPWQYVGERAARADR SEG . xxxxxxxxxxxxxxxxxxxxxxx ...xxxxxxxxxxxxxxxxx PRD ccchhhhhhhhhheeeccccccccchhhhhhhhhhhhhhhhhhhceeeeeehhhhhhhhh SEQ VFVWGFSFSGALGVPΞFVVPSSGPGPRAGARPRRRIQPVPYRLELDQKISSAACGYGFTL SEG xxxxxxxxxxxxxxxxxxxxxxx PRD eeeeccccccccccceeeeeccccccccccccccccccccchhhhhhhheeeccccceee SEQ LΞSKTADVTKVWGMGLNKDSQLGFHRSRKDKTRGYEYVLEPSPVSLPLDRPQETRVLQVS SEG PRD eecccccceeeeccccccccccccccccccccccceeeeeccccccccccccccceeeee SEQ CGRAHSLVLTDREGVFSMGNNSYGQCGRKVVENEIYSESHRVHRMQDFDGQVVQVACGQD SEG PRD cccceeeeeeccceeeeeccccccccccccccccccccccccccccccceeeeeeecccc SEQ HSLFLTDKGEVYSCGWGADGQTGLGHYNITSSPTKLGGDLAGVNVIQVATYGDCCLAVΞA SEG PRD eeeeeecccceeeecccccccccccccccccccccccccccceeeeeeecccceeeeeec SEQ DGGLFGWGNSEYLQLASVTDSTQVNVPRCLHFSGVGKVRQAACGGTGCAVLNGEGHVFVW SEG PRD ccceeeeccccccccccccccccccccccccccccceeeeeccccceeeeeecccceeee SEQ GYGILGKGPNLVESAVPEMIPPTLFGLTEFNPEIQVSRIRCGLSHFAALTNKGELFVWGK SEG PRD cccccccccccccccccccccceeeeeeecccceeeeeeecccceeeeeecccceeeecc SEQ NIRGCLGIGRLEDQYFPWRVTMPGEPVDVACGVDHMVTLAKSFI SEG PRD cccccccccccccccccceeecccceeeeecccccccccccccc Prosite for DKFZphtes3_21d4.1 PΞ00001 200->204 ASN_GLYCOSYLATION PDOC00001 PS00001 268->272 ASN_GLYCOSYLATION PDOC00001 PS00002 17->21 GLYCOSAMINOGLYCAN PDOC00002 PS00002 82->86 GLYCOSAMINOGLYCAN PDOC00002 PS00002 333->337 GLYCOSAMINOGLYCAN PDOC00002 PS00004 14->18 CAMP_PHOSPHO_SITE PDOC00004 PS00005 34->37 PKC_PHOSPHO_SITE PDOC00005 PS00005 122->125 PKC_PHOSPHO_SITE PDOC00005 PS00005 147->150 PKC_PHOSPHO_SITE PDOC00005 PS00005 190->193 PKC_PHOSPHO_SITE PDOC00005 PS00005 219->222 PKC_PHOSPHO_SITE PDOC00005 PS00005 246->249 PKC_PHOSPHO_SITE PDOC00005 PS00005 410->413 PKC_PHOSPHO_SITE PDOC00005 PS00006 34->38 CK2_PHOSPHO_SITE PDOC00006 PS00006 147->151 CK2_PHOSPHO_SITE PDOC00006 PS00006 190->194 CK2_PHOSPHO_SITE PDOC00006 PS00006 290->294 CK2_PHOSPHO_SITE PDOC00006 PS00006 317->321 CK2 PHOSPHO SITE PDOC00006 PS00007 209->217 TYR_PHOSPHO_SITE PDOC00007 PS00007 208->217 TYR_PHOSPHO_SITE PDOC00007 PS00008 9->15 MYRISTYL PDOC00008 PS00008 20->26 MYRISTYL PDOC00008 PS00008 133->139 MYRISTYL PDOC00008 PS00008 238->244 MYRISTYL PDOC00008 PS00008 277->283 MYRISTYL PDOC00008 PS00008 302->308 MYRISTYL PDOC00008 PS00008 344->350 MYRISTYL PDOC00008 PS00009 12->16 AMIDATION PDOC00009 PS00009 206->210 AMIDATION PDOC00009 PΞ00626 179->190 RCC1_2 PDOC00544 PS00626 235->246 RCCl 2 PDOC00544 Pfam for DKFZphtes3_21d4.1 HMM_NAME Regulator of chromosome condensation (RCCl) HMM *IAaGqHHTVCLTqDGRVYtWG* +A GQ+H++ LT++G VY++G Query 235 VACGQDHSLFLTDKGEVYSCG 255 DKFZphtes3_21jl5 group: transcription factors DKFZphtes3_21jl5 encodes a novel 898 ammo acid protein with similarity human NY-CO-33 protein. NY-CO-33 is a protein recognised by autologous antibodies of human colon cancer patients. The novel protein contains 4 C2H2 Zinc fingers and is a new putativ transcription factor. The new protein can find application in modulating/blocking the expression of genes controlled by this transcription factor. strong similarity to "NY-CO-33" complete cDNA, complete eds, potential start at bp 27, EST hits Sequenced by LMU Locus : unknown Insert length: 4407 bp Poly A stretch at pos. 4321, polyadenylation signal at pos. 4301 1 CGCTGCAGCA GGTGTCACAG AGCCGCATGC TCCCGGAGCC CAGCCTCTTC 51 AGCACCGTGC AGCTGTACCG GCAGAGCAGC AAGCTCTATG GCTCCATCTT 101 CACGGGGGCC AGCAAGTTCC GCTGTAAGGA CTGCAGCGCT GCCTACGACA 151 CCCTGGTGGA GTTGACAGTG CACATGAACG AGACGGGGCA TTACCGCGAC 201 GACAACCATG AGACCGATAA CAACAACCCC AAGCGCTGGT CCAAGCCTCG 251 CAAACGCTCC TTGCTGGAAA TGGAAGGGAA GGAAGACGCC CAGAAGGTGC 301 TGAAGTGCAT GTACTGTGGC CACTCCTTTG AGTCCCTGCA GGATTTGAGT 351 GTCCATATGA TCAAAACAAA ACACTACCAA AAAGTGCCTC TGAAGGAACC 401 CGTCACTCCT GTCGCCGCCA AAATCATCCC TGCCACTCGG AAGAAAGCTT 451 CCCTGGAGCT GGAGCTCCCC AGCTCCCCAG ATTCCACAGG TGGAACCCCC 501 AAAGCCACCA TCTCAGACAC CAACGATGCA CTTCAGAAGA ACTCCAACCC 551 TTACATCACG CCAAATAATC GGTACGGCCA CCAGAATGGG GCCAGCTATG 601 CATGGCACTT TGAGGCCCGG AAGTCGCAGA TCCTGAAGTG CATGGAGTGT 651 GGGAGCTCGC ATGACACCCT GCAGGAGCTC ACTGCCCACA TGATGGTCAC 701 TGGCCACTTC ATCAAGGTCA CCAACTCTGC TATGAAAAAG GGGAAGCCCA 751 TTGTGGAGAC GCCTGTCACA CCTACCATCA CAACCCTGCT GGATGAGAAG 801 GTCCAGTCCG TGCCCCTGGC AGCCACCACC TTCACGTCCC CCTCCAATAC 851 ACCTGCCAGC ATCTCCCCAA AACTGAATGT GGAGGTCAAG AAGGAAGTCG 901 ACAAGGAGAA AGCGGTCACT GACGAGAAAC CTAAGCAAAA AGACAAGCCT 951 GGCGAAGAAG AGGAGAAGTG TGACATCTCT TCCAAATACC ATTACTTGAC 1001 TGAAAATGAC TTAGAAGAGA GTCCCAAGGG GGGGCTTGAT ATCCTCAAAT 1051 CCTTGGAAAA CACAGTGACA TCCGCAATCA ACAAGGCCCA GAACGGCACT 1101 CCTAGCTGGG GGGGCTATCC CAGCATCCAT GCCGCCTACC AACTTCCCAA 1151 CATGATGAAG TTGTCCCTGG GCTCGTCGGG GAAGAGCACG CCCCTGAAAC 1201 CCATGTTTGG CAACAGTGAG ATTGTCTCCC CGACGAAAAA CCAGACCCTG 1251 GTCTCTCCAC CCAGCAGCCA GACGTCCCCC ATGCCCAAGA CAAACTTTCA 1301 TGCCATGGAG GAGCTGGTGA AAAAGGTCAC TGAGAAAGTT GCCAAAGTGG 1351 AGGAGAAGAT GAAGGAGCCG GATGGGAAGC TTTCCCCGCC CAAGCGGGCC 1401 ACTCCCTCCC CATGTAGCAG CGAAGTCGGG GAACCCATCA AGATGGAGGC 1451 ATCCAGCGAT GGGGGCTTCC GCAGCCAGGA GAACAGCCCC AGCCCCCCGC 1501 GGGATGGGTG CAAGGATGGG AGCCCCCTCG CTGAGCCGGT GGAGAATGGC 1551 AAGGAGCTGG TGAAGCCCCT AGCCAGCAGT TTGAGTGGCA GCACGGCCAT 1601 CATCACCGAC CACCCGCCTG AACAGCCTTT TGTTAACCCT TTGAGCGCCC 1651 TGCAGTCAGT CATGAACATT CACCTGGGCA AGGCCGCCAA GCCCTCCCTG 1701 CCTGCCCTGG ACCCCATGAG CATGCTTTTC AAGATGAGCA ACAGCCTGGC 1751 GGAGAAGGCT GCTGTGGCCA CCCCGCCGCC CCTGCAGTCC AAGAAGGCAG 1801 ACCACCTCGA CCGCTATTTC TACCACGTCA ACAACGACCA GCCCATAGAC 1851 TTGACAAAAG GGAAGAGTGA CAAAGGCTGC TCCTTGGGTT CAGTGCTTCT 1901 GTCACCCACG TCCACAGCCC CGGCAACCTC CTCATCCACG GTGACAACGG 1951 CAAAGACATC TGCCGTCGTA TCATTCATGT CAAACTCGCC GCTACGCGAG 2001 AATGCCTTGT CAGATATATC CGATATGCTG AAGAACTTGA CAGAGAGCCA 2051 CACGTCAAAA TCCTCCACTC CTTCCAGCAT CTCCGAGAAG TCTGACATTG 2101 ACGGGGCCAC TCTGGAGGAG GCTGAGGAGT CGACGCCCGC CCAGAAGAGG 2151 AAGGGCCGCC AGTCAAACTG GAACCCCCAG CACCTCCTGA TCCTCCAGGC 2201 CCAGTTTGCC GCCAGCCTCC GGCAGACCTC AGAAGGGAAG TACATCATGT 2251 CAGACCTGAG CCCCCAGGAG CGGATGCATA TCTCCAGGTT CACCGGGCTG 2301 TCCATGACCA CCATCAGCCA CTGGCTGGCC AACGTGAAAT ACCAGCTTCG 2351 AAGGACAGGT GGAACAAAGT TCCTCAAAAA CTTGGACACT GGCCACCCCG 2401 TCTTCTTTTG TAACGATTGT GCGTCCCAAA TCAGGACTCC TTCCACGTAC 2451 ATCAGTCACC TAGAGTCACA CTTAGGCTTC CGGCTACGGG ACTTATCCAA 2501 ACTGTCCACC GAACAGATTA ACAGTCAGAT AGCACAAACC AAGTCACCGT 2551 CAGAAAAAAT GGTGACGTCC TCCCCCGAGG AAGACCTGGG GACTTCCTAT 2601 CAGTGCAAAC TTTGCAATCG GACCTTTGCC AGCAAGCACG CTGTTAAACT 2651 TCACCTTAGC AAAACACACG GGAAATCTCC GGAAGACCAC CTTCTGTATG 2701 TCTCTGAGTT AGAGAAGCAG TAGCATTTGC TTTTGATAGA AAGGACTGCA 2751 GTTTGCTTTG AGGGAAACTG TGGAAGGCAC CTTCAGGCCC CCTCTGACTT 2801 GTTGTTCTTG GCACATGTTC TTATTTTAAC TGCAGAGAAT CACTCTGGGC 2851 TGGACTGTTT TGTATAACTG TACAGTGTTT AATAGAGGTG CATAATCAGC 2901 TGTTGTTACT GGTAAAATAT GAAGGTTAAA ATGCAGTGGT AAGTGTTTGG 2951 AACTTTGTGT AAACGGGATT TAGTTGTGAG CATCCTCCCG ATGCTTCAAG 3001 CTGCATGCAT TAACAGACAG TTTAATTAAG CATTTATAAC GGAATCAGGC 3051 ACACCTTTTC CACGAGACTC GAGTGTGCTG GCATTTCTCA CCCTTTCATC 3101 TTTAGCCCTC TGAGTACTTT GAAGCACTTT TGCATTAATT TGGTTAAAAA 3151 ATAAAATAAA ATAATAATAA TGTATGAAGC TCTGTTTTTT AAACTCCTTA 3201 CCAGCTTAGT TATAATGAAT AATATGAACC TCCATTTATG CAGGTCTGCA 3251 GGGGTATAAC ACGCCTTGAA ATTTAAAAGA ATATTATTTT CACATTGAAA 3301 CATAGATGTA TATATTGTAT AGATTTCAGA CTCTCTTATG AAAAAAAATG 3351 TGATTGTGGT TAAATGACCT TTTTCTTGCA TTTATAGCAA CAGTGTTTTA 3401 TGCACCTGCT ATGCTCTGGG CATAAGCTGT GCCTATGTAT AGTGTATATT 3451 TCTTTTTTTC TTTTTTTTAA GGTCTATGGG TTTTGTTTTT TACATGCAAA 3501 CATTGTAAAT TATACAGAAG ATACCACAGA TAGCATTTAT AAAGTATACA 3551 GAAACATTAT CTGAAAGCAA AGTATGATAG TTTGTTTTGC TATACAGTAC 3601 ATCTATATTG ATAGAGGTTC ATGTTTAAAT TATACATATT TATTAGCATC 3651 ATATTGTCAT TTGTTTTGAG CAGTCTGAAT AAACGAGACC GGGAAAGACA 3701 TCCCTGGCAG GCATCAGAAC TATTTTGCAC ATGATTTTTA AAGGTATTTA 3751 TTAGAAATCA AAGAACACTC AAAATAAACT CAGTGCTCAA AGGGTTAAGT 3801 CTATTTGAAA AGGTTAAAAA AAAGAACAAA AAAAAAAAAA GAACTTGTAC 3851 TGTATTTCCT AAACATTGAT AAAGCCTTTA AAATGTTTGT ACTGTAATAC 3901 TTTGCTTAAA AGTCATGAGG CATTCTGTGA TCCAACCTCT TTCACTTATT 3951 TATAAGCCCT CTTGGTTGCT ATTCCATATT GTAGGATGCC TTTCTATTTC 4001 AATTGGTAAC TTTCTGTTTT GTTCTTCCTA ATTATTCTCC CAAGATCCCA 4051 CACTGCAGCT TTATCTTTAG GCTTATGAAA GGTAACCCGT GGTTACCGGC 4101 TCTCCAAGTG ATTCTGTTCT TCTCCATTTT TGGCAGTTAA TTTGCAGAAG 4151 TAACTGACAG CTGACACCAT ATGAGAACCT TTGTATAAAA TATTGGCATG 4201 TAAACAGCAC AGACACCGTA ACACACTCTG TGCCCTGTTT GGTTGTTGAC 4251 AATGAAGCAC CATTATGTGA CTCTTCATAT AACCCTTTTT TCTACGGCAG 4301 CATTAAAATT GTCTTTTTGC TATAAAAAAA AAAAAAAAAA AAAAAAAAAA 4351 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 4401 AAAAAAA BLAST Results No BLAST result Medline entries No Medlme entry Peptide information for frame 3 ORF from 27 bp to 2720 bp; peptide length: 898 Category: strong similarity to known protein 1 MLPEPSLFST VQLYRQSSKL YGSIFTGASK FRCKDCSAAY DTLVELTVHM 51 NETGHYRDDN HETDNNNPKR WSKPRKRSLL EMEGKEDAQK VLKCMYCGHS 101 FESLQDLSVH MIKTKHYQKV PLKEPVTPVA AKIIPATRKK ASLELELPSS 151 PDSTGGTPKA TISDTNDALQ KNSNPYITPN NRYGHQNGAS YAWHFEARKS 201 QILKCMECGS SHDTLQELTA HMMVTGHFIK VTNSAMKKGK PIVETPVTPT 251 ITTLLDEKVQ SVPLAATTFT SPSNTPASIS PKLNVEVKKE VDKEKAVTDE 301 KPKQKDKPGE EEEKCDISSK YHYLTENDLE ESPKGGLDIL KSLENTVTSA 351 INKAQNGTPS WGGYPSIHAA YQLPNMMKLS LGSSGKSTPL KPMFGNSEIV 401 SPTKNQTLVS PPSSQTSPMP KTNFHAMEEL VKKVTEKVAK VEEKMKEPDG 451 KLSPPKRATP SPCSSEVGEP IKMEASSDGG FRSQENSPSP PRDGCKDGSP 501 LAEPVENGKE LVKPLASSLS GSTAIITDHP PEQPFVNPLS ALQSVMNIHL 551 GKAAKPSLPA LDPMSMLFKM SNΞLAEKAAV ATPPPLQSKK ADHLDRYFYH 601 VNNDQPIDLT KGKSDKGCSL GSVLLSPTST APATSSSTVT TAKTSAVVSF 651 MSNSPLRENA LSDISDMLKN LTESHTSKSS TPΞΞISEKSD IDGATLEEAE 701 ESTPAQKRKG RQSNWNPQHL LILQAQFAAS LRQTΞEGK I MSDLSPQERM 751 HISRFTGLSM TTISHWLANV KYQLRRTGGT KFLKNLDTGH PVFFCNDCAS 801 QIRTPSTYIS HLESHLGFRL RDLSKLSTEQ INSQIAQTKS PSEKMVTSSP 851 EEDLGTSYQC KLCNRTFASK HAVKLHLSKT HGKSPEDHLL YVSELEKQ BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_21j 15, frame 3 TREMBL:AF039698_1 gene: "NY-CO-33"; product: "antigen NY-CO-33"; Homo sapiens antigen NY-CO-33 (NY-CO-33) mRNA, complete eds., N = 1, Score = 1039, P = 5.5e-105 PIR:A38437 probable homeotic protein tsh - fruit fly (Drosophila melanogaster), N = 3, Score = 158, P = 7.2e-09 TREMBL:CE33058_1 gene: "unc-89"; product: "UNC-89"; Caenorhabditis elegans UNC-89 (unc-89) gene, complete eds., N = 2, Score = 175, P = 3.3e-07 >TREMBL:AF039698_1 gene: "NY-CO-33"; product: "antigen NY-CO-33"; Homo sapiens antigen NY-CO-33 (NY-CO-33) mRNA, complete eds. Length = 687 HSPs: Score = 1039 (155.9 bits), Expect = 5.5e-105, P = 5.5e-105 Identities = 244/504 (48%), Positives = 319/504 (63%) Query: 170 QKNSNPYITPNNRYGHQNGASYAWHFEARKΞQILKCMECGSSHDTLQELTAHMMVTGHFI 229 QK +NPY+TPNNRYG+QNGASY W FEARK+QILKCMECGSSHDTLQ+LTAHMMVTGHF+ Sbjct: 14 QKAANPYVTPNNRYGYQNGASYTWQFEARKAQILKCMECGSSHDTLQQLTAHMMVTGHFL 73 Query: 230 KVTNSAMKKGKPIVETPVTPTITTLLDEKVQSVPLAATTFTS-PSNT PASISPKLN 284 KVT SA KKGK +V PV ++EK+QS+PL TT T P+++ P S + Sbjct: 74 KVTTSASKKGKQLVLDPV VEEKIQSIPLPPTTHTRLPASSIKKQPDSPAGΞTT 126 Query: 285 VEVKKEVDKEKA-VTDEKPKQKDKPGEEEEKCDISSKYHYLTENDLEESPKGGLDILKSL 343 E KKE +KEK V + K K++ + EK + S+ Y YL E DL++SPKGGLDILKΞL Sbjct: 127 SEEKKEPEKEKPPVAGDAEKIKEEΞEDSLEKFEPSTLYPYLREEDLDDSPKGGLDILKSL 186 Query: 344 ENTVTSAINKAQNGTPSWGGYPSIHAAYQLPNMMKLSLGSSGKSTPLKPMF-GNSEIVSP 402 ENTV++AI+KAQNG PΞWGGYPSIHAAYQLP +K L ++ +S ++P + G + +Ξ Sbjct: 187 ENTVSTAISKAQNGAPSWGGYPSIHAAYQLPGTVK-PLPAAVQSVQVQPSYAGGVKSLΞS 245 Query: 403 TKNQTLVSPPSSQTSPMPKTNFHAMEELVKKVTEKV-AKVEEKMKEPDGKLSPPKRATPS 461 ++ L+ P S T P K+N AMEELV+KVT KV K EE+ E + K S K A S Sbjct: 246 AEHNALLHSPGSLTPPPHKSNVSAMEELVEKVTGKVNIKKEERPPEKE-KSSLAKAA—S 302 Query: 462 PCSSEVGEPIKMEASSDGGFRSQENSPSPPRDGCKDGSPLAEPVENGKELVKPLASSLSG 521 P + E + K E S + Q+ P K PL NG E +K ++ Sbjct: 303 PIAKENKDFPKTEEVSG KPQKKGPEAETWEAKKEGPLDVHTPNGTEPLKAKVTNGCN 359 Query: 522 STAIITDHPPEQPFVNPLSALQSVMNIHLGKAAKPSLPALDPMSMLFKMSNSLAEKAAVA 581 + II DH PE F+NPLSALQS+MN HLGK +KP P+LDP++ML+K+SNS+ +K Sbjct: 360 NLGIIMDHSPEPSFINPLSALQSIMNTHLGKVSKPVSPSLDPLAMLYKISNSMLDKPVYP 419 Query: 582 TPPPLQSKKADHLDRYFYHVNNDQPIDLTKGKSDK-GCSLGSVLLSPTSTAPATSSSTVT 640 P K+AD +DRY+Y N+DQPIDLTK K+ S+ + SP + S + Sbjct: 420 ATPV KQADAIDRYYYE-NSDQPIDLTKSKNKPLVSSVADSVASPLRESALMDISDMV 475 Query: 641 TAKTSAVVΞFMSN-SPLRENALSDISDMLKNLTE 673 T + S S + E + +D Ξ + L E Sbjct: 476 KNLTGRLTPKSSTPSTVSEKSDADGΞSFEEALDE 509 Score = 865 (129.8 bits), Expect = 7.4e-95, P = 7.4e-95 Identities = 211/434 (48%), Positives = 268/434 (61%) Query: 447 EPDGKLSPPKRATPSPCSSEVG—EPIKMEASSDGGFRSQENSPSPPRDG-CKDGSPLAE 503 E + L P TP P S V E + + + + +E P + K SP+A+ Sbjct: 247 EHNALLHSPGSLTPPPHKSNVSAMEELVEKVTGKVNIKKEERPPEKEKSSLAKAASPIAK 306 Query: 504 P-VE—NGKELVK-PLASSLSGSTAIITD-HPPE—QPFVNPLSALQSVMNIHLG 551 P E +GK K P A + D H P +P ++ + + I + Sbjct: 307 ENKDFPKTEEVSGKPQKKGPEAETWEAKKEGPLDVHTPNGTEPLKAKVTNGCNNLGIIMD 366 Query: 552 KAAKPSLPALDPMSMLFKMSNSLAEKAAVATPPPLQSKKADHLDRYFYHVNN DQPID 608 + +PS ++P+S L + N+ K + P L D L Y ++N D+P+ Sbjct: 367 HSPEPSF—INPLSALQSIMNTHLGKVSKPVSPSL DPL-AMLYKISNSMLDKPV- 417 Query: 609 LTKGKSDKGCSLGSVLLSPTSTAPATSSSTVTTAKTSAVVSFMSNSPLRENALSDISDML 668 K S P + + S+V ++ SPLRE+AL DISDM+ Sbjct: 418 -YPATPVKQADAIDRYYYENSDQPIDLTKSKNKPLVSSVADSVA-SPLRESALMDISDMV 475 Query: 669 KNLTESHTΞKSSTPSSISEKΞDIDGATLEEA-EESTPAQKRKGRQSNWNPQHLLILQAQF 727 KNLT T KSSTPS++SEKSD DG++ EEA +E +P KRKGRQSNWNPQHLLILQAQF Sbjct: 476 KNLTGRLTPKSSTPSTVSEKSDADGSSFEEALDELSPVHKRKGRQSNWNPQHLLILQAQF 535 Query: 728 AASLRQTSEGKYIMSDLSPQERMHISRFTGLSMTTISHWLANVKYQLRRTGGTKFLKNLD 787 A+SLR+T+EGKYIMSDL PQER+HIS+FTGLSMTTISHWLANVKYQLRRTGGTKFLKNLD Sbjct: 536 ASSLRETTEGKYIMSDLGPQERVHISKFTGLSMTTISHWLANVKYQLRRTGGTKFLKNLD 595 Query: 788 TGHPVFFCNDCASQIRTPSTYISHLESHLGFRLRDLΞKLSTEQINSQIAQTKSPSEKMV- 846 TGHPVFFCNDCASQ RT STYISHLE+HLGF L+DLSKL QI Q +K + K + Sbjct: 596 TGHPVFFCNDCASQFRTASTYISHLETHLGFSLKDLSKLPLNQIQEQQNVSKVLTNKTLG 655 Query: 847 -TSSPEEDLGTSYQCKLCNRTFASK 870 + EEDLG+++QCKLCNRTFA + Sbjct: 656 PLGATEEDLGSTFQCKLCNRTFAKQ 680 Score = 98 (14.7 bits), Expect = 7.4e-95, P = 7.4e-95 Identities = 32/95 (33%), Positives = 47/95 (49%) Query: 90 KVLKCMYCGHSFESLQDLSVHMIKTKHYQKVPL KEPVT-PVAAKIIPATRKKAS 142 ++LKCM CG S ++LQ L+ HM+ T H+ KV K+ V PV + I + + Sbjct: 45 QILKCMECGSSHDTLQQLTAHMMVTGHFLKVTTSASKKGKQLVLDPVVEEKIQSIPLPPT 104 Query: 143 LELELPSS PDSTGGTPKATISDTNDALQKNSNP 175 LP+S PDS G+ T S+ +K P Sbjct: 105 THTRLPASSIKKQPDSPAGS TTSEEKKEPEKEKPP 139 Score = 81 (12.2 bits), Expect = 4.6e-93, P = 4.6e-93 Identities = 13/29 (44%), Positives = 20/29 (68%) Query: 28 ASKFRCKDCSAAYDTLVELTVHMNETGHY 56 A +C +C +++DTL +LT HM TGH+ Sbjct: 44 AQILKCMECGSSHDTLQQLTAHMMVTGHF 72 Pedant information for DKFZphtes3_21j 15, frame 3 Report for DKFZphtes3_21j 15.3 [LENGTH] 898 [MW] 98486.72 [pi] 8.61 [HOMOL] TREMBL:AF039698_1 gene: "NY-CO-33"; product: "antigen NY-CO-33"; Homo sapiens antigen NY-CO-33 (NY-CO-33) mRNA, complete eds. 0.0 [BLOCKS] BL00028 Zmc finger, C2H2 type, domain proteins [PIRKW] zmc fmger le-06 [PIRKW] DNA binding le-06 [PIRKW] transcription regulation le-06 [PROSITE] MYRISTYL 9 [PROSITE] ZINC_FINGER_C2H2 4 [PROSITE] CAMP_PHOSPHO_SITE 5 [PROSITE] CK2_PHOSPHO_SITE 19 [PROSITE] TYR_PHOSPHO_SITE 2 [PROSITE] PKC_PHOSPHO_SITE 15 [PROSITE] ASN_GLYCOSYLATION 4 [PFAM] Zinc finger, C2H2 type [KW] Alpha_Beta [KW] LOW_COMPLEXITY 11.36 % SEQ MLPEPSLFSTVQLYRQSSKLYGSIFTGASKFRCKDCSAAYDTLVELTVHMNETGHYRDDN SEG PRD ccccceeeeeeeeccccceeeeeeeccccceeecccchhhhhhhhhhhcccccccccccc SEQ HETDNNNPKRWSKPRKRSLLEMEGKEDAQKVLKCMYCGHSFESLQDLSVHMIKTKHYQKV SEG PRD cccccccccccccccchhhhhhhccchhhhhhhhhcccccchhhhheeeeeeeecceeee SEQ PLKEPVTPVAAKIIPATRKKASLELELPSSPDSTGGTPKATISDTNDALQKNSNPYITPN SEG xxxxxxxxxx PRD eccccccceeeeeeehhhhhhhhhhcccccccccccccceeeeccchhhhhccccccccc SEQ NRYGHQNGASYAWHFEARKSQILKCMECGSSHDTLQELTAHMMVTGHFIKVTNSAMKKGK SEG PRD ccccccccchhhhhhhhhhhhhhhhccccccccchhhhhhhhhhhceeeeeccccccccc SEQ PIVETPVTPTITTLLDEKVQSVPLAATTFTSPSNTPASISPKLNVEVKKEVDKEKAVTDE SEG xxxxxxxxxxxxx xxxxxxxxxxxxxxxx PRD ccccccccccchhhhhhhhccccccccccccccccccccccccccccccccchhhhhhcc SEQ KPKQKDKPGEEEEKCDISSKYHYLTENDLEESPKGGLDILKSLENTVTSAINKAQNGTPS SEG x PRD ccccccccccccccchhhhhhhhhhhcccccccccchhhhhhhhhhhhhhhhhhcccccc SEQ WGGYPSIHAAYQLPNMMKLSLGSSGKSTPLKPMFGNSEIVSPTKNQTLVSPPSSQTSPMP SEG PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ KTNFHAMEELVKKVTEKVAKVEEKMKEPDGKLSPPKRATPSPCSSEVGEPIKMEASSDGG SEG xxxxxxxxxxxxxxxxxxxx PRD ccchhhhhhhhhhhhhhhhhhhhhhcccccccccccccccccccccccccceeeeecccc SEQ FRSQENSPSPPRDGCKDGSPLAEPVENGKELVKPLASSLSGSTAIITDHPPEQPFVNPLS SEG PRD cccccccccccccccccccccccccccccccccccccccccceeeeeccccccccccccc SEQ ALQSVMNIHLGKAAKPSLPALDPMSMLFKMSNSLAEKAAVATPPPLQSKKADHLDRYFYH SEG PRD chhhhhhcccccccccccccchhhhhhhhhhhhhhccccccccccccccccccccceeee SEQ VNNDQPIDLTKGKSDKGCSLGSVLLSPTSTAPATSSSTVTTAKTSAVVSFMSNSPLRENA SEG xxxxxxxxxxxxxxxxxxxxxxx PRD ecccccceeecccccccccccceeecccccccccccceeeeceeeeeeeeccccccchhh SEQ LSDISDMLKNLTESHTSKSSTPSSISEKSDIDGATLEEAEESTPAQKRKGRQSNWNPQHL SEG xxxxxxxxxxxxxxxxxx PRD hhhhhhhhhhhhcccccccccccceeecccccchhhhhhhhccchhhhhhcccccccchh SEQ LILQAQFAASLRQTSEGKYIMSDLSPQERMHISRFTGLSMTTISHWLANVKYQLRRTGGT SEG PRD hhhhhhhhhhhhhccccceeecccccchhhhhhhhccccchhhhhhhhhhhhhhhhcccc SEQ KFLKNLDTGHPVFFCNDCASQIRTPSTYISHLESHLGFRLRDLSKLSTEQINSQIAQTKS SEG PRD ceeecccccccceeecccceeeecccchhhhhhhhhhhhhhhhhcchhhhhhhhhhhhcc SEQ PSEKMVTSSPEEDLGTSYQCKLCNRTFASKHAVKLHLSKTHGKSPEDHLLYVSELEKQ SEG PRD ccceeeeccccccccceeehhhhhhhhhhhhhhhhhccccccccccceeeeeeecccc Prosite for DKFZphtes3_21jl5.3 PS00001 51->55 ASN_GLYCOSYLATION PDOC00001 PS00001 405->409 ASN_GLYCOSYLATION PDOC00001 PS00001 670->674 ASN_GLYCOSYLATION PDOC00001 PS00001 864->868 ASN_GLYCOSYLATION PDOC00001 PS00004 69->73 CAMP_PHOSPHO_SITE PDOC00004 PS00004 75->79 CAMP_PHOSPHO_SITE PDOC00004 PS00004 139->143 CAMP_PHOSPHO_SITE PDOC00004 PS00004 432->436 CAMP_PHOSPHO_SITE PDOC00004 PS00004 456->460 CAMP_PHOSPHO_SITE PDOC00004 PS00005 17->20 PKC_PHOSPHO_SI E PDOC00005 PS00005 137->140 PKC_PHOSPHO_SITE PDOC00005 PS00005 157->160 PKC_PHOSPHO_SITE PDOC00005 PS00005 280->283 PKC_PHOSPHO_SITE PDOC00005 PS00005 318->321 PKC_PHOSPHO_SITE PDOC00005 PS00005 332->335 PKC_PHOSPHO_SITE PDOC00005 PS00005 384->387 PKC_PHOSPHO_SITE PDOC00005 PS00005 435->438 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 588->591 PKC_PHOSPHO_SITE PDOC00005 PS00005 614->617 PKC_PHOSPHO_SITE PDOC00005 PS00005 641->644 PKC_PHOSPHO_SITE PDOC00005 PS00005 676->679 PKC_PHOSPHO_SITE PDOC00005 PS00005 686->689 PKC_PHOSPHO_SITE PDOC00005 PS00005 730->733 PKC_PHOSPHO_SITE PDOC00005 PS00005 842->845 PKC_PHOSPHO_SITE PDOC00005 PS00006 42->46 CK2_PHOSPHO_SITE PDOC00006 PS00006 78->82 CK2_PHOSPHO_SITE PDOC00006 PS00006 103->107 CK2_PHOSPHO_SITE PDOC00006 PS00006 149->153 CK2_PHOSPHO_SITE PDOC00006 PS00006 161->165 CK2_PHOSPHO_SITE PDOC00006 PS00006 210->214 CK2_PHOSPHO_SITE PDOC00006 PS00006 214->218 CK2_PHOSPHO_SITE PDOC00006 PS00006 253->257 CK2_PHOSPHO_SITE PDOC00006 PS00006 325->329 CK2_PHOSPHO_SITE PDOC00006 PS00006 573->577 CK2_PHOSPHO_SITE PDOC00006 PS00006 684->688 CK2_PHOSPHO_SITE PDOC00006 PS00006 689->693 CK2_PHOSPHO_SITE PDOC00006 PS00006 695->699 CK2_PHOSPHO_SITE PDOC00006 PS00006 745->749 CK2 PHOSPHO SITE PDOC00006 PS00006 810->814 CK2 PHOSPHO SITE PDOC00006 PS00006 840->844 CK2 PHOSPHO " " SITE PDOC00006 PS00006 848->852 CK2 PHOSPHO " " SITE PDOC00006 PS00006 884->888 CK2 PHOSPHO " SITE PDOC00006 PS00006 893->897 CK2 PHOSPHO " " SITE PDOC00006 PS00007 732->740 TYR PHOSPHO " " SITE PDOC00007 PS00007 883->892 TYR PHOSPHO " " SITE PDOC00007 PS00008 22->28 MYRISTYL PDOC00008 PS00008 156->162 MYRISTYL PDOC00008 PS00008 188->194 MYRISTYL PDOC00008 PS00008 362->368 MYRISTYL PDOC00008 PS00008 479->485 MYRISTYL PDOC00008 PS00008 494->500 MYRISTYL PDOC00008 PS00008 498->504 MYRISTYL PDOC00008 PS00008 617->623 MYRISTYL PDOC00008 PS00008 757->763 MYRISTYL PDOC00008 PS00028 795->816 ZINC FINGER C2H2 PDOC00028 PS00028 860->882 ZINC FINGER " " C2H2 PDOC00028 PS00028 33->56 ZINC FINGER " " C2H2 PDOC00028 PS00028 94->117 ZINC FINGER " " C2H2 PDOC00028 Pfam for DKFZphtes3_21jl5.3 HMM_NAME Zinc finger, C2H2 type HMM *CpwPDCgKtFrrwsNLrRHMR..T.H* C++ C ++ + +L+ HM+ H Query 33 CKD—CSAAYDTLVELTVHMNET-GH 55 26.69 (bits) f: 94 t: 116 Target: dkfzphtes3_21jl5.3 strong similarity to "NY-CO-33" Alignment to HMM consensus : Query *CpwPDCgKtFrrwsNLrRHMR..T.H* C + CG +F + +L HM+ H dkfzphtes3 94 CMY—CGHSFESLQDLSVHMIKT-KH 116 Query f: 795 t: 815 Target: dkfzphtes3_21j 15.3 strong similarity to "NY-CO-33" Alignment to HMM consensus: HMM *CpwPDCgKtFrrwsNLrRHMRTH* C++ C R++S+++ H+ +H Query 795 CND—CASQIRTPSTYISHLESH 815 27.12 (bits) f: 860 t: 881 Target: dkfzphtes3_21j 15.3 strong similarity to "NY-CO-33" Alignment to HMM consensus : Query *CpwPDCgKtFrrwsNLrRHMR.T .H* C+ C++TF +++ + H+ H dkfzphtes3 860 CKL—CNRTFASKHAVKLHLSK-TH 881 DKFZphtes3_21116 group: intracellular transport and trafficking DKFZphtes3_21116 encodes a novel 66 amino acid protein nearly identical to rat ribosome attached membrane protein 4 (ramp4) . The novel protein seems to be the human orthologe of rat ramp 4. Ramp4 is involved in the regulation of translocation of proteins into endoplasmic reticulum, e.g. of the MHC class II associated invariant (gamma) chain. The new protein can find application in modulation of protein translocation into the endoplasmic reticulum. identical to rat ribosome attached membrane protein 4 ORF Bp 316-513 (66 aa) see BLASTX Sequenced by LMU Locus : unknown Insert length: 2488 bp Poly A stretch at pos. 2464, polyadenylation signal at pos. 2442 1 CTTCCTCTTT CACTCCGCGC TCACGGCGGC GGCCAAAGCG GCGGCGACGG 51 CGGCGCGAGA ACGACCCGGC GGCCAGTTCT CTTCCTCCTG CGCACCTGCC 101 CCGCTCGGTC AGTCAGTCGG CGGCCGGCGC CCGGCTTGTG CTCAGACCTC 151 GCGCTTGCGG CGCCCAGGCC CAGCGGCCGT AGCTAGCGTC TGGCCTGAGA 201 ACCTCGGCGC TCCGGCGGCG CGGGCACCAC GAGCCGAGCC TCGCAGCGGC 251 TCCAGAGGAG GCAGGCGAGT GAGCGAGTCC GAGGGGTGGC CGGGGCAGGT 301 GGTGGCGCCG CGAAGATGGT CGCCAAGCAA AGGATCCGTA TGGCCAACGA 351 GAAGCACAGC AAGAACATCA CCCAGCGCGG CAACGTCGCC AAGACCTCGA 401 GAAATGCCCC CGAAGAGAAG GCGTCTGTAG GACCCTGGTT ATTGGCTCTC 451 TTCATTTTTG TTGTCTGTGG TTCTGCAATT TTCCAGATTA TTCAAAGTAT 501 CAGGATGGGC ATGTGAAGTG ACTGACCTTA AGATGTTTCC ATTCTCCTGT 551 GAATTTTAAC TTGAACTCAT TCCTGATGTT TGATACCCTG GTTGAAAACA 601 ATTCAGTAAA GCATCCTGCC TCAGAATGAC TTTCCTATCA TGCTTCATGT 651 GTCATTCCAA GGTTTCTTCA TGAGTCATTC CAAGTTTTCT AGTCCATACC 701 ACAGTGCCTT GCAAAAAACA CCACATGAAT AAAGCAATAA AATTTGATTG 751 TTAAGATACA GTAGTGGACC CTACTTATTC AGTCAATTAA GAGTAAGTTT 801 TTTTATGTGG TTATTAAAAC AGTATGAACA ATTAGTCTAA CTCTGCATAG 851 ACAGGGTCTA GATTTTGTTA ACCCAAATGT ATAACTGCAG TTAGCTTAAA 901 TTACAATTTG AAGTCTTGTG GTTTTTATAT AGCTAGGCAC TTTATTACTC 951 TTTTGAACTG AAAGCACACT CCCTTATAGG TTCATGTAAC TGTCCTGTAA 1001 TAAGGTGCTT ATAAATGGAA CAACTACACA GCCTAGTTTT GCCACAACCT 1051 TTAGCATCTA AAAAGTTTTA AAAGCTTCTA AATGTCTAAT ATAAAGGGAG 1101 ATGCTTATAG CCACAACATC TATTTTACCA ATATTGTTTC CATTACACTA 1151 CCTTGGATTT TGCATGAGTG AGTATAGTAA CCCAAGATGC CATAAAAAAA 1201 AACTTGATCG TTTTCTGACT TAATTAGTTA CTGTGGTTTC ACTAAAAGCT 1251 ACCGTGGTGG AGTGAAGTCA GTCAGGGAAG GTTTGTTTAT GTTACATTTA 1301 TTTCACCAGA ACTATTTTAA TATATCAAAG GGGTTTACTA TGCCAAACAA 1351 AATTCTAGGG AAAAATACTG CTAAAAATGG ATGCCTCATC AGAACATGCT 1401 GTTGAGTCCA ATGTGCCATA AGACATTTTA GCATGTTAAA TAGCACTTTT 1451 AATAGCAAAA AAAGGCACAT CAACTGCGAA GTTATCCTTA GTTTGCAAAT 1501 GCTTTTTCTA GATTAATGAT TTTTCAATCA TTAGGGTACT AGACACATCA 1551 GCCTAAAGTG GCATCTGGAA TTGAATGGAT TTACTGATAA TGATCAGTCT 1601 TTAGTCTTCC CTTTGTTATA TGACTTTATA GGTTATGATT GATCAAATTT 1651 ACGTTTTACT AATGGTAAGG GTGAGGGTCA TAGGGCAGGT TTTGGGTTTT 1701 CTAGTACTGT TGAAAACTGC AAGTATTGGC TATTTGTATA CTTAGCCATA 1751 ACTTGGTGAA AAAAAACCTG AGCAGTGTCT ATGTATTAAT GCGTTGGAAA 1801 GAAAGCTGCT TGTGTTTGCT TTGTTAATTG CCTCAGGATA TTTCTTTTAA 1851 AATAAGCTGT TTTAAGAGGA ACAGAAGGGA AATCTGCTAC CTAGTCTATA 1901 CACAGCGTGA ACCTCACAGG GGGCTTCTGA TACCCTCAAA CATGGAGAAC 1951 AGTAAGGGAG CAGAGTGGTT AAGGACTTTC AGGAACTTAA CTATTCTGGA 2001 ATAAGGAATG AATCAACTGA CCTTGGGCCA GCAGGTTTTT AACTAAATTG 2051 TTACTTGCCT TTCTCACCCA GTTAATCAGT CTCTGTACTT GTTTCCCTTT 2101 TTGAAACAAG TGTCTTGGTT AACTAATTCT GTTTTATGGT TGTGCTAAAT 2151 TCATAGCAGG TGCCTTATTC TTTGCTTTTA GTCAAACCAT TCCATATCAG 2201 AATTTTCCTT GGTTTACTAT AGATATTTGG CTTTAAGTTG TTGTTTGTGT 2251 TTTTTAATGT ACAATGTTCT GATAAATTTG ACTGTTAAAT TGCTATAGCT 2301 AGCAATCATT TTACATATGT AAAAAATTGC ATTCCCTTTG TATTTCATGT 2351 GTAATTCACC AATTAAGTGC AGTTTATATT CAGGTTGGAT TATGCATGTT 2401 TAGGTAAACG AAAGCTGTGT CTTACTTGAT TTATTCTTTA AAAATAAAGT 2451 TCCCTGAATA TTTGAAAAAA AAAAAAAAAA AAAAAAAA BLAST Results Entry HSCDN13 from database EMBL: H. sapiens (TL5) mRNA from LNCaP cell line Score = 1075, P = 5.8e-41, identities = 219/221 Entry AF100470_1 from database TREMBLNEW: gene: "RAMP4"; product: "ribosome attached membrane protein 4"; Rattus norvegicus ribosome attached membrane protein 4 (RAMP4) mRNA, complete eds . Score = 331, P = 3.9e-28, identities = 66/66, positives = 66/66, frame +1 Entry HSG19910 from database EMBL: human STS A002B48. Score = 530, P = 2.1e-17, identities = 108/109 Medline entries No Medline entry Peptide information for frame 1 ORF from 316 bp to 513 bp; peptide length: 66 Category: strong similarity to known protein Classification: Intacellular transport and traffic 1 MVAKQRIRMA NEKHSKNITQ RGNVAKTSRN APEEKASVGP WLLALFIFW 51 CGSAIFQIIQ SIRMGM BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_21116, frame 1 TREMBLNEW:RN0238236_1 gene: "ramp4"; product: "ribosome associated membrane protein RAMP4"; Rattus norvegicus mRNA for ribosome associated membrane protein RAMP4, N = 1, Score = 331, P = 6.2e-30 TREMBL:AF100470_1 gene: "RAMP4"; product: "ribosome attached membrane protein 4"; Rattus norvegicus ribosome attached membrane protein 4 (RAMP4) mRNA, complete eds., N = 1, Score = 331, P = 6.2e-30 >TREMBLNEW:RN0238236_1 gene: "ramp4"; product: "ribosome associated membrane protein RAMP4"; Rattus norvegicus mRNA for ribosome associated membrane protein RAMP4 Length = 75 HSPs: Score = 331 (49.7 bits), Expect = 6.2e-30, P = 6.2e-30 Identities = 66/66 (100%), Positives = 66/66 (100%) Query: 1 MVAKQRIRMANEKHSKNITQRGNVAKTΞRNAPEEKASVGPWLLALFIFVVCGSAIFQIIQ 60 MVAKQRIRMANEKHSKNITQRGNVAKTSRNAPEEKASVGPWLLALFIFVVCGSAIFQIIQ Sbjct: 10 MVAKQRIRMANEKHSKNITQRGNVAKTSRNAPEEKASVGPWLLALFIFVVCGSAIFQIIQ 69 Query: 61 SIRMGM 66 SIRMGM Sbjct: 70 SIRMGM 75 No Pedant data available DKFZphtes3_21n23 group: testes derived DKFZphtes3_15j 18 encodes a novel 148 amino acid protein with strong similarity to rat 7acomp protein. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . strong similarity to rat 7acomp protein on genomic level encoded by AF107885 Sequenced by LMU Locus : /map="14q24.3" Insert length: 3122 bp Poly A stretch at pos. 3070, polyadenylation signal at pos. 3045 1 GGAAAACCTC GTGGGCTCAG CCCGGGAGAA AGGGCCAGGG AAGTTGGGTG 51 GTTCTGTGCT TGGTCTGTCA ATGGAGGAGA TCAAAGTTTT ACGAAGGGTG 101 AAGGAGGAGA ATGATCGGCG AGGTGGATTT ATTCGCATAT TTCCTACATC 151 TGAGACATGG GAAATATATG GGTCCTACCT CGAGCATAAG ACCTCAATGA 201 ACTATATGCT GGCAACACGC CTCTTCCAGG ACAGGGGAAA CCCAAGAAGA 251 AGCTTATTGA CAGGAAGAAC ACGAATGACT GCTGATGGAG CGCCAGAATT 301 GAAGATAGAG AGTCTGAATT CAAAGGCCAA GCTGCATGCT GCACTTTACG 351 AGAGGAAGCT CCTGTCTCTG GAGGTGCGAA AACGTAGACG ACGGAGTAGC 401 AGATTGAGGG CAATGAGGCC AAAATACCCA GTGATTACCC AACCAGCTGA 451 AATGAATGTT AAAACTGAGA CAGAGAGTGA AGAGGAGGAA GAAGTCGCAT 501 TAGATAATGA AGATGAAGAA CAGGAGGCTT CCCAGGAGGA GTCTGCAGGA 551 TTTCTTAGAG AAAATCAAGC CAAATATACA CCCTCATTGA CAGCTTTGGT 601 AGAAAATACA CCCAAAGAAA ATTCCATGAA AGTTCGTGAA TGGAATAATA 651 AAGGTGGACA CTGCTGCAAA CTTGAGACTC AGGAGCTAGA GCCTAAATTT 701 AACCTGATGC AGATTCTTCA AGATAATGGC AATCTTAGCA AAATGCAGGC 751 CCGAATAGCA TTCTCTGCCT ATCTCCAGCA TGTTCAAATT CGCCTGATGA 801 AAGACAGTGG CGGTCAGACG TTCAGTGCCA GTTGGGCTGC CAAAGAGGAT 851 GAACAGATGG AGCTGGTTGT TCGTTTCCTC AAGCGAGCAT CAAATAACCT 901 CCAGCATTCA CTGAGGATGG TATTACCCAG TCGACGATTG GCACTTCTGG 951 AACGCAGAAG AATCCTGGCC CACCAGCTGG GTGACTTTAT CATTGTATAC 1001 AACAAGGAAA CAGAACAAAT GGCTGAAAAG AAATCAAAGA AGAAAGTTGA 1051 GGAAGAAGAG GAAGATGGGG TGAATATGGA AAACTTTCAG GAGTTCATCA 1101 GACAAGCAAG TGAGGCTGAA CTGGAGGAGG TGTTGACTTT TTATACCCAA 1151 AAGAACAAGT CTGCTAGTGT CTTCCTGGGG ACTCACTCTA AAATTTCTAA 1201 GAACAACAAC AATTATTCTG ATAGTGGGGC AAAAGGTGAT CACCCTGAGA 1251 CTATAATGGA AGAAGTGAAA ATAAAGCCAC CTAAACAGCA ACAGACGACA 1301 GAAATTCATT CTGATAAATT ATCTCGATTT ACCACTTCAG CAGAAAAAGA 1351 GGCAAAATTA GTTTATAGCA ATTCCTCCTC TGGTCCTACT GCTACTCTGC 1401 AGAAAATTCC CAACACCCAT TTGTCATCTG TTACAACCTC TGACCTCTCT 1451 CCAGGGCCTT GCCACCATTC TTCTTTATCT CAAATTCCTT CAGCTATCCC 1501 CAGCATGCCT CACCAGCCAA CAATTTTACT GAACACAGTC TCTGCCAGTG 1551 CTTCTCCCTG CCTACATCCC GGGGCACAGA ACATCCCAAG CCCTACTGGC 1601 CTGCCACGCT GTCGATCAGG AAGTCACACC ATTGGTCCCT TTTCTTCCTT 1651 CCAAAGTGCT GCACACATCT ATAGCCAGAA ACTGTCTCGT CCCTCTTCAG 1701 CAAAGGCAGG ATCGTGCTAT CTAAACAAGC ATCATTCAGG AATAGCCAAA 1751 ACACAAAAAG AGGGAGAAGA TGCTTCTTTA TATAGCAAAC GGTACAACCA 1801 AAGTATGGTT ACAGCTGAAC TTCAGCGGCT AGCTGAGAAG CAGGCAGCGA 1851 GACAGTATTC TCCATCCAGC CACATCAACC TCCTCACCCA ACAGGTAACA 1901 AACCTGAATT TGGCAACTGG CATCATAAAC AGAAGCAGTG CTTCAGCTCC 1951 CCCAACCCTC CGACCCATCA TCAGTCCTAG TGGCCCGACA TGGTCTACAC 2001 AGTCAGACCC CCAAGCTCCC GAGAATCACT CCAGCTCTCC TGGAAGCAGG 2051 AGCCTGCAGA CAGGGGGATT TGCCTGGGAA GGAGAAGTAG AAAACAACGT 2101 GTACAGCCAG GCTACAGGGG TGGTCCCCCA GCACAAGTAT CACCCCACAG 2151 CAGGCAGCTA TCAGCTTCAA TTTGCCCTGC AGCAACTTGA ACAACAAAAA 2201 CTTCAGTCCC GGCAGCTCCT GGACCAGAGT CGAGCCCGGC ACCAGGCAAT 2251 CTTTGGCAGC CAGACACTAC CTAACTCCAA TTTATGGACA ATGAATAATG 2301 GTGCAGGTTG TAGAATTTCC AGTGCCACAG CTAGTGGCCA GAAGCCAACC 2351 ACTCTGCCAC AAAAAGTGGT ACCACCTCCA AGTTCTTGCG CCTCCCTGGT 2401 TCCCAAACCC CCACCCAACC ACGAACAAGT GCTCAGAAGG GCAACATCCC 2451 AGAAAGCTTC CAATACCCGC TTCAGATCCT CCTTTCAAAA CTATTTGTGG 2501 TATTTCTTCC AAGCAGTCAG CTGAACTGAG GACGACAGCC TACAAACAAC 2551 TACATGCATC TGAACTGTCT CTTGTAAATG AGCTTTTTTC AGAGCCAGAA 2601 TCATACTCTC CAGGAAATAT GGAGAAAGAA ACCTGAGGAG ATTGAAGTTT 2651 GCCAGGCACA AGGGCAAAAC TCAGACTGAA TGAATTTGAA AGGGTGGGGC 2701 CAAAGATGTT GTAACCTGGG AGACTTCTCT GAAGAAAGAA AACTGTTTAA 2751 GAAACACAGA CTGAACTGCA GTACTTTTCC TTAAATAGCT GAGATGACCT 2801 TCTTTACCCT GGGCTTAGGT GATTCTCATC AGGGTGACCT GAGTGGAAGT 2851 TGGTGGTAAC GACTGTTCTG TGTCAGCACC CAGGACAGTG GTGTCTGTTA 2901 AGGCTGCCAG GGATTAGCAG GGAGGAAAGC CATCAGGACT GGGTAGCCTG 2951 GTAGCACCAA ATCCCAATTA ATGTTACCTG AACATGTGGT GAGGTCAGCC 3001 GTATGATGAA AGATGTTTAA GAGATTAATG TCAGAAGAAT ATGAAAATAA 3051 ACACCGGCTT AAAAAATGTT AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 3101 AAAAAAAAAA AAAAAAAAAA AA BLAST Results Entry AF107885 from database EMBL: Homo sapiens chromosome 14q24.3 clone BAC270M14 transforming growth factor-beta 3 (TGF-beta 3) gene, complete eds; and unknown genes. Score = 3042, P = 3.0e-219, identities = 610/612 5 exons matching 1893-3070 Medline entries No Medline entry Peptide information for frame 2 ORF from 71 bp to 2521 bp; peptide length: 817 Category: strong similarity to known protein 1 MEEIKVLRRV KEENDRRGGF IRIFPTSETW EIYGSYLEHK TSMNYMLATR 51 LFQDRGNPRR SLLTGRTRMT ADGAPELKIE SLNSKAKLHA ALYERKLLSL 101 EVRKRRRRSS RLRAMRPKYP VITQPAEMNV KTETESEEEE EVALDNEDEE 151 QEASQEESAG FLRENQAKYT PSLTALVENT PKENSMKVRE WNNKGGHCCK 201 LETQELEPKF NLMQILQDNG NLSKMQARIA FSAYLQHVQI RLMKDSGGQT 251 FSASWAAKED EQMELVVRFL KRASNNLQHS LRMVLPΞRRL ALLERRRILA 301 HQLGDFIIVY NKETEQMAEK KSKKKVEEEE EDGVNMENFQ EFIRQASEAE 351 LEEVLTFYTQ KNKSASVFLG THSKISKNNN NYSDSGAKGD HPETIMEEVK 401 IKPPKQQQTT EIHSDKLSRF TTSAEKEAKL VYSNSSSGPT ATLQKIPNTH 451 LSSVTTSDLS PGPCHHSSLS QIPSAIPSMP HQPTILLNTV SASASPCLHP 501 GAQNIPSPTG LPRCRSGSHT IGPFSSFQSA AHIYSQKLΞR PSSAKAGSCY 551 LNKHHSGIAK TQKEGEDASL YSKRYNQΞMV TAELQRLAEK QAARQYSPSS 601 HINLLTQQVT NLNLATGIIN RSSASAPPTL RPIISPSGPT WSTQSDPQAP 651 ENHSSSPGSR SLQTGGFAWE GEVENNVYSQ ATGVVPQHKY HPTAGSYQLQ 701 FALQQLEQQK LQSRQLLDQS RARHQAIFGS QTLPNSNLWT MNNGAGCRIS 751 SATASGQKPT TLPQKVVPPP SSCASLVPKP PPNHEQVLRR ATSQKASNTR 801 FRSSFQNYLW YFFQAVS BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_21n23, frame 2 TREMBL:AF064856_1 product: "7acomp protein"; Rattus sp. 7acomp protein mRNA, complete eds., N = 1, Score = 1845, P = 2.2e-190 TREMBL:AF107885_3 product: "unknown"; Homo sapiens chromosome 14q24.3 clone BAC270M14 transforming growth factor-beta 3 (TGF-beta 3) gene, complete eds; and unknown genes., N = 1, Score = 443, P = 5.3e-41 TREMBL:AF107885_4 product: "unknown"; Homo sapiens chromosome 14q24.3 clone BAC270M14 transforming growth factor-beta 3 (TGF-beta 3) gene, complete eds; and unknown genes., N = 1, Score = 265, P = 8.2e-22 >TREMBL : AF064856_1 product: "7acomp protein"; Rattus sp. 7acomp protein mRNA, complete eds. Length = 436 HSPs: Score = 1845 (276.8 bits), Expect = 2.2e-190, P = 2.2e-190 Identities = 369/435 (84%), Positives = 395/435 (90%) Query: 115 MRPKYPVITQPAEMNVKTETESEEEEEVALDNEDEEQEASQEESAGFLRENQAKYTPSLT 174 MRPKYPVIT PAEMN+KTETESEEEEEV LDNEDEEQEASQEESAG L ENQAKYTPSLT Sb j ct: 1 MRPKYPVITLPAEMNIKTETESEEEEEVGLDNEDEEQEASQEESAGSLAENQAKYTPSLT 60 Query: 175 ALVENTPKENSMKVREWNNKGGHCCKLETQELEPKFNLMQILQDNGNLSKMQARIAFSAY 234 +VEN+P+EN+MKV EW NKG CCK+ETQE E KFNLMQILQDNGNLSK+QAR+AFSAY Sb j ct: 61 VIVENSPRENAMKVAEWTNKGESCCKIETQEPESKFNLMQILQDNGNLSKVQARLAFSAY 120 Query: 235 LQHVQIRLMKDSGGQTFSAΞWAAKEDEQMELVVRFLKRASNNLQHSLRMVLPSRRLALLE 294 LQHVQ+RL KDSGGQT S SWAAKEDEQMELVVRFLKRAS+NLQHSLRMVLPΞRRLALLE Sbjct: 121 LQHVQVRLTKDSGGQTLSPSWAAKEDEQMELVVRFLKRASSNLQHSLRMVLPSRRLALLE 180 Query: 295 RRRILAHQLGDFIIVYNKETEQMAEKKSKKKVEEEEEDGVNMENFQEFIRQASEAELEEV 354 RRRILAHQLGDFI+VYNKETEQMAEKKΞKKK+EEEEEDGVN E+FQEFIRQASEAELEEV Sb j ct: 181 RRRILAHQLGDFIVVYNKETEQMAEKKSKKKLEEEEEDGVNAESFQEFIRQASEAELEEV 240 Query: 355 LTFYTQKNKSASVFLGTHSKISKNNNNYSDSGAKGDHPETIMEEVKIKPPKQQQTTEIHS 414 LTFYTQKNKSASVFLGTHSK SKN+++YSDSGAKGDHPETI +EVKIK PKQQQ TEIHS Sbjct: 241 LTFYTQKNKΞASVFLGTHSKSSKNSSSYSDSGAKGDHPETI-QEVKIKQPKQQQATEIHS 299 Query: 415 DKLSRFTTSAEKEAKLVYSNSSS--GPTATL-QKIPNTHLSSV-TTSDLSPGPCHHSSLS 470 DKLSRFTTSA KEAKLVY+N SS GP A L Q++P+THLSS+ TTS LS GP HHΞSLS Sbjct: 300 DKLSRFTTSAGKEAKLVYTNCSSFSGPAAVLLQRLPSTHLSSIITTSTLSSGPGHHSSLS 359 Query: 471 QIPSAIPSMPHQPTILLNTVSASASPCLHPGAQNIPSPTGLPRCRSGSHTIGPFSSFQSA 530 QI AIPSMPHQ +LLN V SASP +HPG N+ SP GLPRCRSGS+TIGPFSSFQSA Sbjct: 360 QISPAIPSMPHQSALLLNPVPDSAΞPPVHPGTPNV-SPAGLPRCRSGSYTIGPFΞΞFQSA 418 Query: 531 AHIYSQKLSRPSSAKAG 547 AHIYSQKLSRPSSAKAG Sbjct: 419 AHIYSQKLSRPSSAKAG 435 Pedant information for DKFZphtes3_21n23, frame 2 Report for DKFZphtes3_21n23.2 [LENGTH] 817 [MW] 91522.09 [pi] 9.32 [HOMOL] TREMBL: AF064856_1 product: "7acomp protein"; Rattus sp. 7acomp protein mRNA, complete eds. le-166 [PROSITE] MYRISTYL 6 [PROSITE] CAMP_PHOSPHO_SITE 4 [PROSITE] CK2_PHOSPHO_SITE 12 [PROSITE] TYR_PHOSPHO_SITE 1 [PROSITE] PKC_PHOSPHO_SITE 15 [PROSITE] ASN_GLYCOSYLATION 7 [KW] Alpha_Beta [KW] LOW COMPLEXITY 13.83 % SEQ MEEIKVLRRVKEENDRRGGFIRIFPTSETWEIYGSYLEHKTSMNYMLATRLFQDRGNPRR SEG PRD ccchhhhhhhhhhhccccceeeecccccceeeecceeeecccchhhhhhhhhhhcccccc SEQ SLLTGRTRMTADGAPELKIESLNSKAKLHAALYERKLLSLEVRKRRRRSΞRLRAMRPKYP SEG xxxxxxxxxxxxxxxxxxxx PRD ccccccceeeccccceeeeehhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccc SEQ VITQPAEMNVKTETESEEEEEVALDNEDEEQEASQEESAGFLRENQAKYTPSLTALVENT SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD ceeeccchhhhhhhhhhhhhhhhhcccchhhhhhhhhhhhhhhhhhhccccceeeeeccc SEQ PKENΞMKVREWNNKGGHCCKLETQELEPKFNLMQILQDNGNLSKMQARIAFSAYLQHVQI SEG PRD cccccceeeeeccccccccchhhhhhhccchhhhhhhcccchhhhhhhhhhhhhhhhhhh SEQ RLMKDSGGQTFSASWAAKEDEQMELVVRFLKRASNNLQHSLRMVLPSRRLALLERRRILA SEG xxxxxxxxxxxxxxx . PRD hhhhcccccceeehhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccchhhhhhhhhhhhh SEQ HQLGDFIIVYNKETEQMAEKKSKKKVEEEEEDGVNMENFQEFIRQASEAELEEVLTFYTQ SEG xxxxxxxxxxxxx PRD hhccceeeeeehhhhhhhhhhhhhhhhhhhccccchhhhhhhhhhhhhhhhhhhhhhhhh SEQ KNKSASVFLGTHSKISKNNNNYSDSGAKGDHPETIMEEVKIKPPKQQQTTEIHSDKLSRF SEG PRD ccccceeeecccccccccccccccccccccccchhhhhhhccccccceeeeecccccccc SEQ TTSAEKEAKLVYSNSSΞGPTATLQKIPNTHLSSVTTSDLSPGPCHHSSLSQIPSAIPSMP SEG PRD hhhhhhhheeeecccccccceeeecccccccccccccccccccccccccccccccccccc SEQ HQPTILLNTVSASASPCLHPGAQNIPSPTGLPRCRSGSHTIGPFSSFQSAAHIYSQKLSR SEG PRD cccceeeeccccccccccccccccccccccccccccccccccccccchhhhhhhhhhccc SEQ PSSAKAGΞCYLNKHHSGIAKTQKEGEDASLYSKRYNQSMVTAELQRLAEKQAARQYSPSS SEG PRD cccccccceeeecccccccccccccccceeeecchhhhhhhhhhhhhhhhhhhhhhcccc SEQ HINLLTQQVTNLNLATGIINRSSASAPPTLRPIISPSGPTWSTQSDPQAPENHSSSPGSR SEG .. xxxxxxxxxxxx PRD cccccccccccccccccccccccccccccceeeecccccccccccccccccccccccccc SEQ SLQTGGFAWEGEVENNVYSQATGVVPQHKYHPTAGSYQLQFALQQLEQQKLQSRQLLDQS SEG xxxxxxxxxxxxxxxxxxx ... PRD cccccccceeeeecccccccccccccccccccccchhhhhhhhhhhhhhhhhhhhhhhhh SEQ RARHQAIFGSQTLPNSNLWTMNNGAGCRISSATASGQKPTTLPQKVVPPPΞSCASLVPKP SEG PRD hhhhhhhhccccccccceeeeccccceeeeeeeccccccccccceeecccccceeecccc SEQ PPNHEQVLRRATSQKASNTRFRSSFQNYLWYFFQAVS SEG PRD cccchhhhhhhhhhhcccccccccccceeeeeeeccc Prosite for DKFZphtes3_21n23.2 PS00001 221->225 ASNJ3LYCOSYLATION PDOC00001 PS00001 362->366 ASN_GLYCOSYLATION PDOC00001 PS00001 381->385 ASN_GLYCOSYLATION PDOC00001 PS00001 434->438 ASN_GLYCOSYLATION PDOC00001 PS00001 576->580 ASN_GLYCOSYLATION PDOC00001 PS00001 620->624 ASN_GLYCOSYLATION PDOC00001 PS00001 652->656 ASN_GLYCOSYLATION PDOC00001 PS00004 106->110 CAMP_PHOSPHO_SITE PDOC00004 PS00004 107->111 CAMP_PHOSPHO_SITE PDOC00004 PS00004 271->275 CAMP_PHOSPHO_SITE PDOC00004 PS00004 789->793 CAMP_PHOSPHO_SITE PDOC00004 PS00005 64->67 PKC_PHOSPHO_SITE PDOC00005 PS00005 109-M12 PKC_PHOSPHO_SITE PDOC00005 PS00005 180->183 PKC_PHOSPHO_SITE PDOC00005 PS00005 185->188 PKC_PHOSPHO_SITE PDOC00005 PS00005 280->283 PKC_PHOSPHO_SITE PDOC00005 PS00005 287->290 PKC_PHOSPHO_SITE PDOC00005 PS00005 322->325 PKC_PHOSPHO_SITE PDOC00005 PS00005 359->362 PKC_PHOSPHO_SITE PDOC00005 PS00005 414->417 PKC_PHOSPHO_SITE PDOC00005 PS00005 535->538 PKC_PHOSPHO_SITE PDOC00005 PS00005 543->546 PKC_PHOSPHO_SITE PDOC00005 PS00005 561->564 PKC_PHOSPHO_SITE PDOC00005 PS00005 572->575 PKC_PHOSPHO_SITE PDOC00005 PS00005 629->632 PKC_PHOSPHO_SITE PDOC00005 PS00005 793->796 PKC_PHOSPHO_SITE PDOC00005 PS00006 35->39 CK2_PHOSPHO_SITE PDOC00006 PS00006 132->136 CK2_PHOSPHO_SITE PDOC00006 PS00006 134->138 CK2_PHOSPHO_SITE PDOC00006 PS00006 136->140 CK2_PHOSPHO_SITE PDOC00006 PS00006 154->158 CK2_PHOSPHO_SITE PDOC00006 PS00006 180->184 CK2_PHOSPHO_SITE PDOC00006 PS00006 347->351 CK2_PHOSPHO_SITE PDOC00006 PS00006 394->398 CK2_PHOSPHO_SITE PDOC00006 PS00006 422->426 CK2_PHOΞPHO_SITE PDOC00006 PS00006 455->459 CK2_PHOSPHO_SITE PDOC00006 PS00006 561->565 CK2_PHOSPHO_SITE PDOC00006 PS00006 643->647 CK2_PHOSPHO_SITE PDOC00006 PS00007 563->572 TYR_PHOSPHO_SITE PDOC00007 PS00008 195->201 MYRISTYL PDOC00008 PS00008 248->254 MYRISTYL PDOC00008 PS00008 510->516 MYRISTYL PDOC00008 PS00008 557->563 MYRISTYL PDOC00008 PS00008 746->752 MYRISTYL PDOC00008 PS00008 756->762 MYRISTYL PDOC00008 (No Pfam data available for DKFZphtes3_21n23.2) DKFZphtes3_22c23 group: testes derived DKFZphtes3_22c23 encodes a novel 223 amino acid protein without similarity to known proteins. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . unknown complete cDNA, complete eds, 3 EST hits (two from a testis library) Sequenced by LMU Locus: /map="9q34" Insert length: 1113 bp Poly A stretch at pos. 1073, polyadenylation signal at pos. 1055 1 GGTGGGCAAA GGCATCTTCC TCTGGGAAGG ACTGGCACAA GCACTTGGTC 51 CCTGGGTTGT GTGCCTGGGA GGCCGGGATC AGGGCTGGCC CTCTTTCTCC 101 CTGGCAAAGC AAAACCTCCC TTTTACTACT ATCAAGGGGA AGTAACTTGA 151 AGGTGCCTGT GGCAGGCAGC ACCTTGAGCC AACAGGAACC ATTGACATGC 201 GAGGCCCAGG GCAGGCAGAC TGTGCAGTGG CCATTGGGCG GCCCCTCGGG 251 GAGGTGGTGA CCCTCCGCGT CCTTGAGAGT TCTCTCAACT GCAGTGCGGG 301 GGACATGTTG CTGCTTTGGG GCCGGCTCAC CTGGAGGAAG ATGTGCAGGA 351 AGCTGTTGGA CATGACTTTC AGCTCCAAGA CCAACACGCT GGTGGTGAGG 401 CAGCGCTGCG GGCGGCCAGG AGGTGGGGTG CTGCTGCGGT ATGGGAGCCA 451 GCTTGCTCCT GAAACCTTCT ACAGAGAATG TGACATGCAG CTCTTTGGGC 501 CCTGGGGTGA AATCGTGAGC CCCTCGCTGA GTCCAGCCAC GAGTAATGCA 551 GGGGGCTGCC GGCTCTTCAT TAATGTGGCT CCGCACGCAC GGATTGCCAT 601 CCATGCCCTG GCCACCAACA TGGGCGCTGG GACCGAGGGA GCCAATGCCA 651 GCTACATCTT GATCCGGGAC ACCCACAGCT TGAGGACCAC AGCGTTCCAT 701 GGGCAGCAGG TGCTCTACTG GGAGTCAGAG AGCAGCCAGG CTGAGATGGA 751 GTTCAGCGAG GGCTTCCTGA AGGCTCAGGC CAGCCTGCGG GGCCAGTACT 801 GGACCCTCCA ATCATGGGTA CCGGAGATGC AGGACCCTCA GTCCTGGAAG 851 GGAAAGGAAG GAACCTGAGG GTCATTGAAC ATTTGTTCCG TGTCTGGCCA 901 GCCCTGGAGG GTTGACCCCT GGTCTCAGTG CTTTCCAATT CGAACTTTTT 951 CCAATCTTAG GTATCTACTT TAGAGTCTTC TCCAATGTCC AAAAGGCTAG 1001 GGGGTTGGAG GTGGGGACTC TGGAAAAGCA GCCCCCATTT CCTCGGGTAC 1051 CAATAAATAA AACATGCAGG CTGAAAAAAA AAAAAAAAAA AAAAAAAAAA 1101 AAAAAAAAAA AAA BLAST Results Entry HSAC1644 from database EMBL: Genomic sequence from Human 9q34, complete sequence. Score = 2072, P = 8.8e-225, identities = 422/430 5 exons Bp 41969-38232 Medline entries No Medlme entry Peptide information for frame 2 ORF from 197 bp to 865 bp; peptide length: 223 Category: putative protein 1 MRGPGQADCA VAIGRPLGEV VTLRVLESSL NCSAGDMLLL WGRLTWRKMC 51 RKLLDMTFSS KTNTLVVRQR CGRPGGGVLL RYGSQLAPET FYRECDMQLF 101 GPWGEIVSPS LSPATSNAGG CRLFINVAPH ARIAIHALAT NMGAGTEGAN 151 ASYILIRDTH SLRTTAFHGQ QVLYWESESS QAEMEFSEGF LKAQASLRGQ 201 YWTLQSWVPE MQDPQSWKGK EGT BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_22c23, frame 2 No Alert BLASTP hits found Pedant information for DKFZphtes3_22c23, frame 2 Report for DKFZphtes3_22c23.2 [LENGTH] 223 [MW] 24546.19 [pi] 8.57 [PROSITE] MYRISTYL 4 [PROSITE] CK2_PHOΞPHO_SITE [PROSITE] PKC_PHOSPHO_SI E [PROSITE] ASN_GLYCOSYLATION [KW] Alpha_Beta SEQ MRGPGQADCAVAIGRPLGEVVTLRVLESSLNCSAGDMLLLWGRLTWRKMCRKLLDMTFSS PRD ccccccccceeeecccccceeeeehhhhhcccccchhhhhhchhhhhhhhhhhhhhhccc SEQ KTNTLVVRQRCGRPGGGVLLRYGSQLAPETFYRECDMQLFGPWGEIVSPSLSPATSNAGG PRD ccceeeeeecccccccceeeeccccccchhhhhhhhhccccccceeeecccccccccccc SEQ CRLFINVAPHARIAIHALATNMGAGTEGANASYILIRDTHSLRTTAFHGQQVLYWESESS PRD ceeeeeecccceeehhhhhhhhccccccccceeeeeecccccceeecccceeeeeccccc SEQ QAEMEFSEGFLKAQASLRGQYWTLQSWVPEMQDPQSWKGKEGT PRD hhhhhhhcchhhhhhhhhhcccccccccccccccccccccccc Prosite for DKFZphtes3_22c23.2 PS00001 31->35 ASN_GLYCOSYLATION PDOC00001 PS00001 150->154 ASN_GLYCOSYLATION PDOC00001 PS00005 22->25 PKC_PHOSPHO_SITE PDOC00005 PS00005 45->48 PKC_PHOSPHO_SITE PDOC00005 PS00005 59->62 PKC_PHOSPHO_SITE PDOC00005 PS00005 161->164 PKC_PHOSPHO_SITE PDOC00005 PS00005 19θ->199 PKC_PHOSPHO_SITE PDOC00005 PS00005 216->219 PKC_PHOSPHO_SITE PDOC00005 PS00006 33->37 CK2_PHOSPHO_SITE PDOC00006 PS00006 180->184 CK2_PHOSPHO_SITE PDOC00006 PS00008 5->ll MYRISTYL PDOC00008 PS00008 145->151 MYRISTYL PDOC00008 PS00008 148->154 MYRISTYL PDOC00008 PS00008 199->205 MYRISTYL PDOC00008 (No Pfam data available for DKFZphtes3_22c23.2) DKFZphtes3_22g2 group: nucleic acid management DKFZphtes3_22g2encodes a novel 1230 ammo acid protein with nearly identical to rat TIP120. TATA-binding protein TBP is a central component for transcriptional regulation and is a target for various transcription regulators. TBP-interacting protein 120 (TIP120) is a protein interacting with the TATA-binding protein (TBP) . The novel protein is the human ortholog of rat TIP120. The novel TBP-binding protein is considered to participate in transcription regulation through the interaction with TBP. The new protein can find application m modulation of gene transcription. KIAA0829, complete eds, nearly identical to rat TIP120 complete cDNA, complete eds, EST hits, Sequenced by LMU Locus: /map="387.3 cR from top of Chrl2 linkage group" Insert length: 5387 bp Poly A stretch at pos. 5352, polyadenylation signal at pos. 5335 1 GGGAGCGAGT GCGGAGCGAG TGGGAGCGAG ACGGCCCTGA GTGGAAGTGT 51 CTGGCTCCCC GTAGAGGCCC TTCTGTACGC CCCGCCGCCC ATGAGCTCGT 101 TCTCACGCGA ACAGCGCCGT CGTTAGGCTG GCTCTGTAGC CTCGGCTTAC 151 CCCGGGACAG GCCCACGCCT CGCCAGGGAG GGGGCAGCCC GTCGAGGCGC 201 CTCCCTAGTC AGCGTCGGCG TCGCGCTGCG ACCCTGGAAG CGGGAGCCGC 251 CGCGAGCGAG AGGAGGAGCT CCAGTGGCGG CGGCGGCGGC GGCAGCGGCA 301 GCGGGCAGCA GCTCCAGCAG CGCCAGCAGG CGGGATCGAG GCCGTCAACA 351 TGGCGAGCGC CTCGTACCAC ATTTCCAATT TGCTGGAAAA AATGACATCC 401 AGCGACAAGG ACTTTAGGTT TATGGCTACA AATGATTTGA TGACGGAACT 451 GCAGAAAGAT TCCATCAAGT TGGATGATGA TAGTGAAAGG AAAGTAGTGA 501 AAATGATTTT GAAGTTATTG GAAGATAAAA ATGGAGAGGT ACAGAATTTA 551 GCTGTCAAAT GTCTTGGTCC TTTAGTGAGT AAAGTGAAAG AATACCAAGT 601 AGAGACAATT GTAGATACCC TCTGCACTAA CATGCTTTCT GATAAAGAAC 651 AACTTCGAGA CATTTCAAGT ATTGGTCTTA AAACAGTAAT TGGAGAACTT 701 CCTCCAGCTT CCAGTGGCTC TGCATTAGCT GCTAATGTAT GTAAAAAGAT 751 TACTGGACGT CTTACAAGTG CAATAGCAAA ACAGGAAGAT GTCTCTGTTC 801 AGCTAGAAGC CTTGGATATT ATGGCTGATA TGTTGAGCAG GCAAGGAGGA 851 CTTCTTGTTA ATTTCCATCC TTCAATTCTG ACCTGTCTAC TTCCCCAGTT 901 GACCAGCCCT AGACTTGCAG TGAGGAAAAG AACCATTATC GCTCTTGGCC 951 ATCTGGTTAT GAGCTGTGGA AATATAGTTT TTGTAGATCT TATTGAACAT 1001 CTGTTGTCAG AGTTGTCCAA AAATGATTCT ATGTCAACAA CAAGAACCTA 1051 CATACAATGT ATTGCTGCTA TTAGTAGGCA AGCTGGTCAT AGAATAGGTG 1101 AATACCTTGA GAAGATAATT CCTTTGGTGG TAAAATTTTG CAATGTAGAT 1151 GATGATGAAT TAAGAGAGTA CTGTATTCAA GCCTTTGAAT CATTTGTAAG 1201 AAGATGTCCT AAGGAAGTAT ATCCTCATGT TTCTACCATT ATAAATATTT 1251 GTCTTAAATA TCTTACCTAT GATCCAAATT ATAATTACGA TGATGAAGAT 1301 GAAGATGAAA ATGCAATGGA TGCTGATGGT GGTGATGATG ATGATCAAGG 1351 GAGTGATGAT GAATACAGTG ATGATGATGA CATGAGTTGG AAAGTGAGAC 1401 GTGCAGCTGC GAAGTGCTTG GATGCTGTAG TTAGCACAAG GCATGAAATG 1451 CTTCCAGAAT TCTACAAGAC CGTCTCTCCT GCACTAATAT CCAGATTTAA 1501 AGAGCGTGAA GAGAATGTAA AGGCAGATGT TTTTCACGCA TACCTTTCTC 1551 TTTTGAAGCA AACTCGTCCT GTACAAAGTT GGCTATGTGA CCCTGATGCA 1601 ATGGAGCAGG GAGAAACACC TTTAACAATG CTTCAGAGTC AGGTTCCCAA 1651 CATTGTTAAA GCTCTTCACA AACAGATGAA AGAAAAAAGT GTGAAGACCC 1701 GACAGTGTTG TTTTAACATG TTAACTGAGC TGGTAAATGT ATTACCTGGG 1751 GCCCTAACTC AACACATTCC TGTACTTGTA CCAGGAATCA TTTTCTCACT 1801 GAATGATAAA TCAAGCTCAT CGAATTTGAA GATCGATGCT TTGTCATGTC 1851 TATACGTAAT CCTCTGTAAC CATTCTCCTC AAGTCTTCCA TCCTCACGTT 1901 CAGGCTTTGG TTCCTCCAGT GGTGGCTTGT GTTGGAGACC CATTTTACAA 1951 AATTACATCT GAAGCACTTC TTGTTACTCA ACAGCTTGTC AAAGTAATTC 2001 GTCCTTTAGA TCAGCCTTCC TCGTTTGATG CAACTCCTTA TATCAAAGAT 2051 CTATTTACCT GTACCATTAA GAGATTAAAA GCAGCTGACA TTGATCAGGA 2101 AGTCAAGGAA AGGGCTATTT CCTGTATGGG ACAAATTATT TGCAACCTTG 2151 GAGACAATTT GGGTTCTGAC TTGCCTAATA CACTTCAGAT TTTCTTGGAG 2201 AGACTAAAGA ATGAAATTAC CAGGTTAACT ACAGTAAAGG CATTGACACT 2251 GATTGCTGGG TCACCTTTGA AGATAGATTT GAGGCCTGTT CTGGGAGAAG 2301 GGGTTCCTAT CCTTGCTTCA TTTCTTAGAA AAAACCAGAG AGCTTTGAAA 2351 CTGGGTACTC TTTCTGCCCT TGATATTCTA ATAAAAAACT ATAGTGACAG 2401 CTTGACAGCT GCCATGATTG ATGCAGTTCT AGATGAGCTC CCACCTCTTA 2451 TCAGCGAAAG TGATATGCAT GTTTCACAAA TGGCCATCAG TTTTCTTACC 2501 ACTTTGGCAA AAGTATATCC CTCCTCCCTT TCAAAGATAA GTGGATCCAT 2551 TCTCAATGAA CTTATTGGAC TTGTGAGATC ACCCTTATTG CAGGGGGGAG 2601 CTCTTAGTGC CATGCTAGAC TTTTTCCAAG CTCTGGTTGT CACTGGAACA 2651 AATAATTTAG GATACATGGA TTTGTTGCGC ATGCTGACTG GTCCAGTTTA 2701 CTCTCAGAGC ACAGCTCTTA CTCATAAGCA GTCTTATTAT TCCATTGCCA 2751 AATGTGTAGC TGCCCTTACT CGAGCATGCC CTAAAGAGGG ACCAGCTGTA 2801 GTAGGTCAGT TTATTCAAGA TGTCAAGAAC TCAAGGTCTA CAGATTCCAT 2851 TCGTCTCTTA GCTCTACTTT CTCTTGGAGA AGTTGGGCAT CATATTGACT 2901 TAAGTGGACA GTTGGAACTA AAATCTGTAA TACTAGAAGC TTTCTCATCT 2951 CCTAGTGAAG AAGTCAAATC AGCTGCATCC TATGCATTAG GCAGCATTAG 3001 TGTGGGCAAC CTTCCTGAAT ATCTGCCGTT TGTCCTGCAA GAAATAACTA 3051 GTCAACCCAA AAGGCAGTAT CTTTTACTTC ATTCCTTGAA GGAAATTATT 3101 AGCTCTGCAT CAGTGGTGGG CCTTAAACCA TATGTTGAAA ACATCTGGGC 3151 CTTATTACTA AAGCACTGTG AGTGTGCAGA GGAAGGAACC AGAAATGTTG 3201 TTGCTGAATG TCTAGGAAAA CTCACTCTAA TTGATCCAGA AACTCTCCTT 3251 CCACGGCTTA AGGGGTACTT GATATCAGGC TCATCATATG CCCGAAGCTC 3301 AGTGGTTACG GCTGTGAAAT TTACAATTTC TGACCATCCA CAACCTATTG 3351 ATCCACTGTT AAAGAACTGC ATAGGTGATT TCCTAAAAAC TTTGGAAGAC 3401 CCAGATTTGA ATGTGAGAAG AGTAGCCTTG GTCACATTTA ATTCAGCAGC 3451 ACATAACAAG CCATCATTAA TAAGGGATCT ATTGGATACT GTTCTTCCAC 3501 ATCTTTACAA TGAAACAAAA GTTAGAAAGG AGCTTATAAG AGAGGTAGAA 3551 ATGGGTCCAT TTAAACATAC GGTTGATGAT GGTCTGGATA TTAGAAAGGC 3601 AGCATTTGAG TGTATGTACA CACTTCTAGA CAGTTGTCTT GATAGACTTG 3651 ATATCTTTGA ATTTCTAAAT CATGTTGAAG ATGGTTTGAA GGACCATTAT 3701 GATATTAAGA TGCTGACATT TTTAATGTTG GTGAGACTGT CTACCCTTTG 3751 TCCAAGTGCA GTACTGCAGA GGTTGGACCG ACTTGTTGAG CCATTACGTG 3801 CAACATGTAC AACTAAGGTA AAGGCAAACT CAGTAAAGCA GGAGTTTGAA 3851 AAACAAGATG AATTAAAGCG ATCTGCCATG AGAGCAGTAG CAGCACTGCT 3901 AACCATTCCA GAAGCAGAGA AGAGTCCACT GATGAGTGAA TTCCAGTCAC 3951 AGATCAGTTC TAACCCTGAG CTGGCGGCTA TCTTTGAAAG TATCCAGAAA 4001 GATTCATCAT CTACTAACTT GGAATCAATG GACACTAGTT AGATGTTTGT 4051 TCACCATGGG GACCATTACA TATGACCATA CAATGCACTG AATTGACAGG 4101 TTAATCATAA GACATGGAAA GAGAAGTGTC TAAAAGCTTC AAAATGTTCC 4151 ACTTTTTTTT CCTTCATGGA GACTGTTTGT TTGGCTTTCT TCCATTGTTG 4201 TTTTTGTAGC ATTTATTTCA GAAATGTGTA TTTCCATAAT CCAGAGGTTG 4251 TAAAACCACT AGTGTTTTAG TGGTTACAGC AACATTTGAA ATGGAAACTA 4301 AAAGTTAGGA TTTTATGGAG TATGGAGATA GGGTCCAGTA TCTATTTACC 4351 CTGTAATGTT TAGGATTAAA ATGTTAAAAT TTTGTGACCA TGAATTTCTT 4401 TCTTTTATAA ATTTTCTCAT TTAAAAATCA AAAATCTTGC AAAACAAAAA 4451 CCATGTTTCT TTTTCTTGTA TAACTTTTTG TTTTCAGCAA CATAAATTGA 4501 TTTTTAGCTG GCAGACAAGA ATATCCATAT AAGATTTGTT AACCATTTCA 4551 GAGAGTTTGG CAATTTTTAA AAGATAATAA GGTATCATTT TTAAGTATGA 4601 AAATTAACAA TATCCCTGTT GCGCACACTA ATTTTGCATG AGTAAGTTTA 4651 CAAATATGTA TCGTCTGTAA AGCAGCATGT GCAGATTATT CATAATATAG 4701 AAGTTAAAAT AAGTATTAGT GCAATTTTCA GATATTTATT TTTGCACAGA 4751 AAACACATTA TCTGGAGAGA AAGAAAGGAG AATTTTTGAG ACTTGGGTTT 4801 TCTTAATGCC AGTGTGAATT TGCAGATGTT TTCAGAAAAT CAAGTCACAG 4851 TAACAATTTG CCACTTTTTT CTATTATAAA TCTTCTTACT TAAATTTTGA 4901 ATATTTAGTT TTTCTCAGTT ACCCATTTGT GTGTGTGTGA TTCCACTTAG 4951 AAATTCTTAA AACCAGATTT TTCTTTCATT CCGTTTGGAT GTCTACATTC 5001 CTTATCAAAG GATATAAATA CTGTGTATGC TTTTGAATTT TATTTTTAGG 5051 AAAATTCTGA AGCCAGCTAT CACAGGTTTG TTAGCTAATA ATAGTATTTT 5101 CTTTTAGTTG AGTTAGGTTT TTCCCCATCT CCTGTAGAGC GAATTTACAT 5151 ATTGTATTGG GTAAGTGTTC ACTACTTTTC CTGATTAAGG GATCTGTGCT 5201 GGGGAACAAA GCTTTTGCAG TACCTTATAT TGTAGTTAAA ATTTTATTTA 5251 ACATATCCTT CAGTGAGCTC ATTTCACACT GTAGCCTCTT CCTTAAAATT 5301 TGTGGTGCTC CTGTAACAGT AAGAACTAAT TCTGAAATAA AAGACATCTC 5351 CTAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAA BLAST Results Entry HS793345 from database EMBL: human STS WI-12457. Score = 1985, P = 1.3e-83, identities = 433/460 Medline entries 97127450: Molecular cloning of a novel 120-kDa TBP-mteracting protein. Peptide information for frame 2 ORF from 350 bp to 4039 bp; peptide length: 1230 Category: known protein Classification: Nucleic acid management 1 MASASYHISN LLEKMTSSDK DFRFMATNDL MTELQKDSIK LDDDΞERKVV 51 KMILKLLEDK NGEVQNLAVK CLGPLVSKVK EYQVETIVDT LCTNMLSDKE 101 QLRDISSIGL KTVIGELPPA SSGSALAANV CKKITGRLTS AIAKQEDVSV 151 QLEALDIMAD MLSRQGGLLV NFHPSILTCL LPQLTSPRLA VRKRTIIALG 201 HLVMSCGNIV FVDLIEHLLS ELSKNDSMST TRTYIQCIAA ISRQAGHRIG 251 EYLEKIIPLV VKFCNVDDDE LREYCIQAFE SFVRRCPKEV YPHVSTIINI 301 CLKYLTYDPN YNYDDEDEDE NAMDADGGDD DDQGΞDDEYS DDDDMSWKVR 351 RAAAKCLDAV VSTRHEMLPE FYKTVSPALI SRFKEREENV KADVFHAYLS 401 LLKQTRPVQS WLCDPDAMEQ GETPLTMLQS QVPNIVKALH KQMKEKSVKT 451 RQCCFNMLTE LVNVLPGALT QHIPVLVPGI IFSLNDKSSΞ SNLKIDALSC 501 LYVILCNHSP QVFHPHVQAL VPPVVACVGD PFYKITSEAL LVTQQLVKVI 551 RPLDQPSSFD ATPYIKDLFT CTIKRLKAAD IDQEVKERAI SCMGQIICNL 601 GDNLGSDLPN TLQIFLERLK NEITRLTTVK ALTLIAGSPL KIDLRPVLGE 651 GVPILASFLR KNQRALKLGT LSALDILIKN YSDSLTAAMI DAVLDELPPL 701 ISESDMHVSQ MAISFLTTLA KVYPΞSLSKI SGSILNELIG LVRSPLLQGG 751 ALSAMLDFFQ ALVVTGTNNL GYMDLLRMLT GPVYSQSTAL THKQSYYSIA 801 KCVAALTRAC PKEGPAVVGQ FIQDVKNSRS TDΞIRLLALL SLGEVGHHID 851 LSGQLELKSV ILEAFSSPSE EVKSAASYAL GSISVGNLPE YLPFVLQEIT 901 SQPKRQYLLL HSLKEIISSA SVVGLKPYVE NIWALLLKHC ECAEEGTRNV 951 VAECLGKLTL IDPETLLPRL KGYLISGSSY ARSSVVTAVK FTISDHPQPI 1001 DPLLKNCIGD FLKTLEDPDL NVRRVALVTF NSAAHNKPSL IRDLLDTVLP 1051 HLYNETKVRK ELIREVEMGP FKHTVDDGLD IRKAAFECMY TLLDSCLDRL 1101 DIFEFLNHVE DGLKDHYDIK MLTFLMLVRL STLCPSAVLQ RLDRLVEPLR 1151 ATCTTKVKAN SVKQEFEKQD ELKRΞAMRAV AALLTIPEAE KSPLMSEFQS 1201 QISSNPELAA IFESIQKDSΞ STNLESMDTS BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_22g2, frame 2 TREMBL:AB020636_1 gene: "KIAA0829"; product: "KIAA0829 protein"; Homo sapiens mRNA for KIAA0829 protein, partial eds., N = 1, Score = 5986, P = 0 TREMBL :RND6711_1 gene: "tιpl20"; product: "TIP120"; Rattus norvegicus mRNA for TIP120, complete eds., N = 1, Score = 6203, P = 0 >TREMBL:RND6711_1 gene: "tιpl20"; product: "TIP120"; Rattus norvegicus mRNA for TIP120, complete eds. Length = 1,230 HSPs: Score = 6203 (930.7 bits), Expect = O.Oe+00, P = O.Oe+00 Identities = 1227/1230 (99%), Positives = 1228/1230 (99%) Query: 1 MASASYHISNLLEKMTSSDKDFRFMATNDLMTELQKDSIKLDDDSERKVVKMILKLLEDK 60 MASASYHISNLLEKMTSSDKDFRFMATNDLMTELQKDSIKLDDDSERKVVKMILKLLEDK Sbjct: 1 MASASYHISNLLEKMTSSDKDFRFMATNDLMTELQKDSIKLDDDSERKVVKMILKLLEDK 60 Query: 61 NGEVQNLAVKCLGPLVSKVKEYQVETIVDTLCTNMLSDKEQLRDISSIGLKTVIGELPPA 120 NGEVQNLAVKCLGPLVSKVKEYQVETIVDTLCTNMLΞDKEQLRDISSIGLKTVIGELPPA Sbjct: 61 NGEVQNLAVKCLGPLVSKVKEYQVETIVDTLCTNMLSDKEQLRDISSIGLKTVIGELPPA 120 Query: 121 SSGSALAANVCKKITGRLTSAIAKQEDVSVQLEALDIMADMLSRQGGLLVNFHPSILTCL 180 SSGSALAANVCKKITGRLTSAIAKQEDVSVQLEALDIMADMLSRQGGLLVNFHPSILTCL Sbjct: 121 SSGSALAANVCKKITGRLTSAIAKQEDVSVQLEALDIMADMLSRQGGLLVNFHPSILTCL 180 Query: 181 LPQLTSPRLAVRKRTIIALGHLVMSCGNIVFVDLIEHLLSELSKNDSMSTTRTYIQCIAA 240 LPQLTSPRLAVRKRTIIALGHLVMΞCGNIVFVDLIEHLLSELSKNDSMSTTRTYIQCIAA Sbjct: 181 LPQLTSPRLAVRKRTIIALGHLVMSCGNIVFVDLIEHLLSELSKNDSMSTTRTYIQCIAA 240 Query: 241 ISRQAGHRIGEYLEKIIPLVVKFCNVDDDELREYCIQAFEΞFVRRCPKEVYPHVSTIINI 300 ISRQAGHRIGEYLEKIIPLVVKFCNVDDDELREYCIQAFEΞFVRRCPKEVYPHVSTIINI Sbjct: 241 ISRQAGHRIGEYLEKIIPLVVKFCNVDDDELREYCIQAFESFVRRCPKEVYPHVSTIINI 300 Query: 301 CLKYLTYDPNYNYDDEDEDENAMDADGGDDDDQGSDDEYSDDDDMSWKVRRAAAKCLDAV 360 CLKYLTYDPNYNYDDEDEDENAMDADGGDDDDQGSDDEYSDDDDMSWKVRRAAAKCLDAV Sbjct: 301 CLKYLTYDPNYNYDDEDEDENAMDADGGDDDDQGSDDEYSDDDDMSWKVRRAAAKCLDAV 360 Query: 361 VSTRHEMLPEFYKTVSPALISRFKEREENVKADVFHAYLSLLKQTRPVQSWLCDPDAMEQ 420 VSTRHEMLPEFYKTVSPALISRFKEREENVKADVFHAYLSLLKQTRPVQSWLCDPDAMEQ Sb j ct: 361 VSTRHEMLPEFYKTVSPALISRFKEREENVKADVFHAYLSLLKQTRPVQSWLCDPDAMEQ 420 Query: 421 GETPLTMLQSQVPNIVKALHKQMKEKSVKTRQCCFNMLTELVNVLPGALTQHIPVLVPGI 480 GETPLTMLQSQVPNIVKALHKQMKEKSVKTRQCCFNMLTELVNVLPGALTQHIPVLVPGI Sbjct: 421 GETPLTMLQSQVPNIVKALHKQMKEKSVKTRQCCFNMLTELVNVLPGALTQHIPVLVPGI 480 Query: 481 IFSLNDKSSSSNLKIDALSCLYVILCNHSPQVFHPHVQALVPPVVACVGDPFYKITSEAL 540 IFSLNDKSSΞSNLKIDALSCLYVILCNHSPQVFHPHVQALVPPVVACVGDPFYKITSEAL Sbjct: 481 IFSLNDKSSSSNLKIDALSCLYVILCNHSPQVFHPHVQALVPPVVACVGDPFYKITSEAL 540 Query: 541 LVTQQLVKVIRPLDQPSSFDATPYIKDLFTCTIKRLKAADIDQEVKERAISCMGQIICNL 600 LVTQQLVKVIRPLDQPSSFDATPYIKDLFTCTIKRLKAADIDQEVKERAISCMGQIICNL Sbjct: 541 LVTQQLVKVIRPLDQPSSFDATPYIKDLFTCTIKRLKAADIDQEVKERAIΞCMGQIICNL 600 Query: 601 GDNLGSDLPNTLQIFLERLKNEITRLTTVKALTLIAGSPLKIDLRPVLGEGVPILASFLR 660 GDNLG DL NTLQIFLERLKNEITRLTTVKALTLIAGSPLKIDLRPVLGEGVPILASFLR Sbjct: 601 GDNLGPDLSNTLQIFLERLKNEITRLTTVKALTLIAGSPLKIDLRPVLGEGVPILASFLR 660 Query: 661 KNQRALKLGTLSALDILIKNYSDSLTAAMIDAVLDELPPLISESDMHVSQMAISFLTTLA 720 KNQRALKLGTLSALDILIKNYΞDSLTAAMIDAVLDELPPLISESDMHVSQMAISFLTTLA Sbjct: 661 KNQRALKLGTLSALDILIKNYSDSLTAAMIDAVLDELPPLISEΞDMHVSQMAISFLTTLA 720 Query: 721 KVYPSSLSKISGSILNELIGLVRSPLLQGGALSAMLDFFQALVVTGTNNLGYMDLLRMLT 780 KVYPSΞLSKISGSILNELIGLVRSPLLQGGALSAMLDFFQALVVTGTNNLGYMDLLRMLT Sbjct: 721 KVYPSSLSKISGSILNELIGLVRSPLLQGGALSAMLDFFQALVVTGTNNLGYMDLLRMLT 780 Query: 781 GPVYSQSTALTHKQSYYSIAKCVAALTRACPKEGPAVVGQFIQDVKNSRSTDSIRLLALL 840 GPVYSQSTALTHKQSYYSIAKCVAALTRACPKEGPAVVGQFIQDVKNSRSTDSIRLLALL Sbjct: 781 GPVYSQΞTALTHKQSYYSIAKCVAALTRACPKEGPAVVGQFIQDVKNSRSTDSIRLLALL 840 Query: 841 SLGEVGHHIDLSGQLELKSVILEAFSSPSEEVKSAASYALGSISVGNLPEYLPFVLQEIT 900 SLGEVGHHIDLSGQLELKSVILEAFSSPSEEVKSAASYALGSISVGNLPEYLPFVLQEIT Sbjct: 841 SLGEVGHHIDLSGQLELKSVILEAFSSPSEEVKSAASYALGSISVGNLPEYLPFVLQEIT 900 Query: 901 SQPKRQYLLLHSLKEIISSASVVGLKPYVENIWALLLKHCECAEEGTRNVVAECLGKLTL 960 SQPKRQYLLLHSLKEIISSASVVGLKPYVENIWALLLKHCECAEEGTRNVVAECLGKLTL Sbjct: 901 SQPKRQYLLLHSLKEI ISSASVVGLKPYVENIWALLLKHCECAEEGTRNVVAECLGKLTL 960 Query: 961 IDPETLLPRLKGYLISGSSYARSSVVTAVKFTISDHPQPIDPLLKNCIGDFLKTLEDPDL 1020 IDPETLLPRLKGYLISGSSYARSSVVTAVKFTISDHPQPIDPLLKNCIGDFLKTLEDPDL Sbjct: 961 IDPETLLPRLKGYLISGSSYARSSVVTAVKFTISDHPQPIDPLLKNCIGDFLKTLEDPDL 1020 Query: 1021 NVRRVALVTFNSAAHNKPSLIRDLLDTVLPHLYNETKVRKELIREVEMGPFKHTVDDGLD 1080 NVRRVALVTFNSAAHNKPSLIRDLLD+VLPHLYNETKVRKELIREVEMGPFKHTVDDGLD Sbjct: 1021 NVRRVALVTFNSAAHNKPSLIRDLLDSVLPHLYNETKVRKELIREVEMGPFKHTVDDGLD 1080 Query: 1081 IRKAAFECMYTLLDSCLDRLDIFEFLNHVEDGLKDHYDIKMLTFLMLVRLΞTLCPSAVLQ 1140 IRKAAFECMYTLLDSCLDRLDIFEFLNHVEDGLKDHYDIKMLTFLMLVRLSTLCPSAVLQ Sbjct: 1081 IRKAAFECMYTLLDSCLDRLDIFEFLNHVEDGLKDHYDIKMLTFLMLVRLSTLCPSAVLQ 1140 Query: 1141 RLDRLVEPLRATCTTKVKANSVKQEFEKQDELKRSAMRAVAALLTIPEAEKSPLMSEFQS 1200 RLDRLVEPLRATCTTKVKANSVKQEFEKQDELKRSAMRAVAALLTI PEAEKSPLMSEFQS Sbjct: 1141 RLDRLVEPLRATCTTKVKANSVKQEFEKQDELKRSAMRAVAALLTIPEAEKSPLMSEFQS 1200 Query: 1201 QISSNPELAAIFESIQKDSSSTNLESMDTS 1230 QISSNPELAAIFESIQKDSSSTNLESMDTS Sbjct: 1201 QISSNPELAAIFESIQKDSSSTNLESMDTS 1230 Pedant information for DKFZphtes3_22g2, frame 2 Report for DKFZphtes3_22g2.2 [LENGTH] 1230 [MW] 136376.58 [pi] 5.52 [HOMOL] TREMBL :RND6711_1 gene: "tιpl20"; product: "TIP120"; Rattus norvegicus mRNA for TIP120, complete eds. 0.0 [KW] TRANSMEMBRANE 1 [KW] LOW_COMPLEXITY 5.28 % SEQ MASASYHISNLLEKMTSSDKDFRFMATNDLMTELQKDSIKLDDDSERKVVKMILKLLEDK SEG PRD cccccchhhhhhhhhcccccceeeeehhhhhhhhhcccccccccchhhhhhhhhhhhhcc MEM SEQ NGEVQNLAVKCLGPLVSKVKEYQVETIVDTLCTNMLSDKEQLRDISSIGLKTVIGELPPA SEG xxxx PRD ccccceeeeeeeeceeeeehhhhhhhhhhhhccchhhhhcccccccchhhhhhhhhcccc MEM SEQ SSGSALAANVCKKITGRLTSAIAKQEDVSVQLEALDIMADMLSRQGGLLVNFHPSILTCL SEG xxxxxxxx PRD cccccchhhhhhhccchhhhhhhccccchhhhhhhhhhhhhhhhhccceeeecchhhhhh MEM SEQ LPQLTSPRLAVRKRTIIALGHLVMSCGNIVFVDLIEHLLSELSKNDSMSTTRTYIQCIAA SEG PRD hcccccchhhhhhhhhhhheeeeecccceeehhhhhhhhhhhhccccchhhhhhhhhhhh MEM MMMMMMMMMMMMMMMMM SEQ ISRQAGHRIGEYLEKIIPLVVKFCNVDDDELREYCIQAFESFVRRCPKEVYPHVSTIINI SEG PRD hhhhcccccccchhhhhhhhheeeeccchhhhhhhhhhhhhhhhccccceeecchhhhhh MEM SEQ CLKYLTYDPNYNYDDEDEDENAMDADGGDDDDQGSDDEYSDDDDMSWKVRRAAAKCLDAV SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD hhhhhccccccccccccccccccccccccccccccccccccccchhhhhhhhhhhhhhhh MEM SEQ VSTRHEMLPEFYKTVSPALISRFKEREENVKADVFHAYLSLLKQTRPVQSWLCDPDAMEQ SEG PRD hhhhhhhhhhhhhhhhchhhhhhhhhhhhhhhhhhhhhhhhhhhhccceeeecccccccc MEM SEQ GETPLTMLQSQVPNIVKALHKQMKEKSVKTRQCCFNMLTELVNVLPGALTQHIPVLVPGI SEG PRD cccchhhhhhhhhhhhhhhhhhhhhhhhhcccchhhhhhhhhhhccccccccceeeecce MEM SEQ IFSLNDKSSSSNLKIDALSCLYVILCNHSPQVFHPHVQALVPPVVACVGDPFYKITSEAL SEG xxxxxxxxxxxxxxxx PRD eeeeccccccccchhhhhhhheeeeecccccccccceeeeecceeeeecccchhhhhhhh MEM SEQ LVTQQLVKVIRPLDQPSSFDATPYIKDLFTCTIKRLKAADIDQEVKERAISCMGQIICNL SEG PRD hhhhhhhhhhcccccccccccccchhhhhhhhhhhhhhccchhhhhhhhhhhhheeeecc MEM SEQ GDNLGSDLPNTLQIFLERLKNEITRLTTVKALTLIAGSPLKIDLRPVLGEGVPILASFLR SEG PRD cccccccccchhhhhhhhhcchhhhhhhhhhhheeeeccccccccceeehhhhhhhhhhh MEM SEQ KNQRALKLGTLSALDILIKNYSDSLTAAMIDAVLDELPPLISESDMHVSQMAISFLTTLA SEG PRD hhhhhhhhhhhhhhhhhhhccccchhhhhhhhhhhhcccccccchhhhhhhhhhhhhhhh MEM SEQ KVYPSSLSKISGSILNELIGLVRSPLLQGGALSAMLDFFQALVVTGTNNLGYMDLLRMLT SEG PRD cccccceeecchhhhhhhhhhhccccccchhhhhhhhhhhheeeecccccchhhhhhhhc MEM SEQ GPVYSQSTALTHKQSYYSIAKCVAALTRACPKEGPAVVGQFIQDVKNSRSTDSIRLLALL SEG PRD cccccccccchhhhhhhhhhhhhhhhhhhhcccchhhhhhhhhhhhcccccchhhhhhhh MEM SEQ SLGEVGHHIDLSGQLELKSVILEAFSSPSEEVKSAASYALGSISVGNLPEYLPFVLQEIT SEG PRD hccccccccccccccccceeeeeeccccchhhhhhhhhhhccccccccccchhhhhhhhh MEM SEQ SQPKRQYLLLHSLKEIISSAΞVVGLKPYVENIWALLLKHCECAEEGTRNVVAECLGKLTL SEG PRD cccchhhhhhhhhhhhhhcccceeehhhhhhhhhhhhhhhhcccccceeeeecccccccc MEM SEQ IDPETLLPRLKGYLISGSSYARSSVVTAVKFTIΞDHPQPIDPLLKNCIGDFLKTLEDPDL SEG PRD cccccccccccccccccccccchhhhhhhhhhhccccccccccchhhhhhhhhhhccccc MEM SEQ NVRRVALVTFNSAAHNKPSLIRDLLDTVLPHLYNETKVRKELIREVEMGPFKHTVDDGLD SEG PRD ccceeeeeeecccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccccccccccch MEM SEQ IRKAAFECMYTLLDSCLDRLDIFEFLNHVEDGLKDHYDIKMLTFLMLVRLSTLCPSAVLQ SEG PRD hhhhhhhhhhhhhhhccccccceeeecccccccccchhhhhhhhhhhhhhhhcccchhhh MEM SEQ RLDRLVEPLRATCTTKVKANSVKQEFEKQDELKRSAMRAVAALLTIPEAEKSPLMSEFQS SEG PRD hhhhhhhhhhhhhccccchhhhhhhhhhhhhhhhhhhhhhhhhhhcccccccccchhhhh MEM SEQ QISSNPELAAIFESIQKDSSSTNLESMDTS SEG PRD hhhccchhhhhhhhhhhccccccccccccc MEM (No Prosite data available for DKFZphtes3_22g2.2) (No Pfam data available for DKFZphtes3_22g2.2) DKFZphtes3_22nl3 group: testes derived DKFZphtes3_22nl3 encodes a novel 677 ammo acid protein without similarity to known proteins. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . dJ1042K10.3, complete Sequenced by LMU Locus: /map="22ql3.1-13.2" Insert length: 3353 bp Poly A stretch at pos. 3315, polyadenylation signal at pos. 3298 1 ATGGAACCAC TATCCCCACT GCCAAGTCCA CCCCCACACT CATTAAGCAA 51 AGCCAACCCA AGTCTGCCAG TGAGAAGTCA CAGCGCAGCA AGAAGGCCAA 101 GGAGCTGAAG CCAAAGGTGA AGAAGCTCAA GTACCACCAG TACATCCCCC 151 CGGACCAGAA GCAGGACAGG GGGGCACCCC CCATGGACTC ATCCTACGCC 201 AAGATCCTGC AGCAGCAGCA GCTCTTCCTC CAGCTGCAGA TCCTCAACCA 251 GCAGCAGCAG CAGCACCACA ACTACCAGGC CATCCTGCCT GCCCCGCCAA 301 AGTCAGCAGG CGAGGCCCTG GGAAGCAGCG GGACCCCCCC AGTACGCAGC 351 CTCTCCACTA CCAATAGCAG CTCCAGCTCG GGCGCCCCTG GGCCCTGTGG 401 GCTGGCACGT CAGAACAGCA CCTCACTGAC TGGCAAGCCG GGAGCCCTGC 451 CGGCCAACCT GGACGACATG AAGGTGGCAG AGCTGAAGCA GGAGCTGAAG 501 TTGCGATCAC TGCCTGTCTC GGGCACCAAA ACTGAGCTGA TTGAGCGCCT 551 TCGAGCCTAT CAAGACCAAA TCAGCCCTGT GCCAGGAGCC CCCAAGGCCC 601 CTGCCGCCAC CTCTATCCTG CACAAGGCTG GCGAGGTGGT GGTAGCCTTC 651 CCAGCGGCCC GGCTGAGCAC GGGGCCAGCC CTGGTGGCAG CAGGCCTGGC 701 TCCAGCTGAG GTGGTGGTGG CCACGGTGGC CAGCAGTGGG GTGGTGAAGT 751 TTGGCAGCAC GGGCTCCACG CCCCCCGTGT CTCCCACCCC CTCGGAGCGC 801 TCACTGCTCA GCACGGGCGA TGAAAACTCC ACCCCCGGGG ACACCTTTGG 851 TGAGATGGTG ACATCACCTC TGACGCAGCT GACCCTGCAG GCCTCGCCAC 901 TGCAGATCCT CGTGAAGGAG GAGGGCCCCC GGGCCGGGTC CTGTTGCCTG 951 AGCCCTGGGG GGCGGGCGGA GCTAGAGGGG CGCGACAAGG ACCAGATGCT 1001 GCAGGAGAAA GACAAGCAGA TCGAGGCGCT GACGCGCATG CTCCGGCAGA 1051 AGCAGCAGCT GGTGGAGCGG CTCAAGCTGC AGCTGGAGCA GGAGAAGCGA 1101 GCCCAGCAGC CCGCCCCCGC CCCCGCCCCC CTCGGCACCC CCGTGAAGCA 1151 GGAGAACAGC TTCTCCAGCT GCCAGCTGAG CCAGCAGCCC CTGGGCCCCG 1201 CTCACCCATT CAACCCCAGC CTGGCGGCCC CAGCCACCAA CCACATAGAC 1251 CCTTGTGCTG TGGCCCCAGG GCCCCCGTCC GTGGTGGTGA AGCAGGAAGC 1301 CTTGCAGCCT GAGCCCGAGC CGGTCCCCGC CCCCCAGTTG CTTCTGGGGC 1351 CTCAGGGCCC CGGCCTCATC AAGGGGGTTG CACCTCCCAC CCTCATCACC 1401 GACTCCACAG GGACCCACCT TGTCCTCACC GTGACCAATA AGAATGCAGA 1451 CAGCCCTGGC CTGTCCAGTG GGAGCCCCCA GCAGCCCTCG TCCCAGCCTG 1501 GCTCTCCAGC GCCTGCCCCC TCTGCCCAGA TGGACCTGGA GCACCCACTG 1551 CAGCCCCTCT TTGGGACCCC CACTTCTCTG CTGAAGAAGG AACCACCTGG 1601 CTATGAGGAA GCCATGAGCC AGCAGCCCAA ACAGCAGGAA AATGGTTCCT 1651 CAAGCCAGCA GATGGACGAC CTGTTTGACA TTCTCATTCA GAGCGGAGAA 1701 ATTTCAGCAG ATTTCAAGGA GCCGCCATCC CTGCCAGGGA AGGAGAAGCC 1751 ATCCCCGAAG ACAGTCTGTG GGTCCCCCCT GGCAGCACAG CCATCACCTT 1801 CTGCTGAGCT CCCCCAGGCT GCCCCACCTC CTCCAGGCTC ACCCTCCCTC 1851 CCTGGACGCC TGGAGGACTT CCTGGAGAGC AGCACGGGGC TGCCCCTGCT 1901 GACCAGTGGG CATGACGGGC CAGAGCCCCT TTCCCTCATT GACGACCTCC 1951 ATAGCCAGAT GCTGAGCAGC ACTGCCATCC TGGACCACCC CCCGTCACCC 2001 ATGGACACCT CGGAATTGCA CTTTGTTCCT GAGCCCAGCA GCACCATGGG 2051 CCTGGACCTG GCTGATGGCC ACCTGGACAG CATGGACTGG CTGGAGCTGT 2101 CGTCAGGTGG TCCCGTGCTG AGCCTAGCCC CCCTCAGCAC CACAGCCCCC 2151 AGCCTCTTCT CCACAGACTT CCTCGATGGC CATGATTTGC AGCTGCACTG 2201 GGATTCCTGC TTGTAGCTCT CTGGCTCAAG ACGGGGTGGG GAAGGGGCTG 2251 GGAGCCAGGG TACTCCAATG CGTGGCTCTC CTGCGTGATT CGGCCTCTCC 2301 ACATGGTTGT GAGTCTTGAC AATCACAGCC CCTGCTTTTT CCCTTCCCTG 2351 GGAGGCTAGA ACAGAGAAGC CCTTACTCCT GGTTCAGTGC CACGCAGGGC 2401 AGAGGAGAGC AGCTGTCAAG AAGCAGCCCT GGCTCTCACG CTGGGGTTTT 2451 GGACACACGG TCAGGGTCAG GGCCATTTCA GCTTGACCTC CTTTTTTGAG 2501 GTCAGGGGGC ACTGTCTGTC TGGCTACAAT TTGGCTAAGG TAGGTGAAGC 2551 CTGGCCAGGC GGGAGGCTTC TCTTCTGACC CAGGGCTGAG ACAGGTTAAG 2601 GGGTGAATCT CCTTCCTTTC TCTCCCTGCT TTGCTGTGAA GGGAGAAATT 2651 AGCCTGGGCC TCTACCCCCT ATTCCCTGTG TCTGCCAACC CCAGGATCCC 2701 AGGGCTCCCT GCCATTTTAG TGTCTTGGTG TAGTGTAACC ATTTAGTGGT 2751 TGGTGGCAAC AATTTTATGT ACAGGTGTAT ATACCTCTAT ATTATATATC 2801 GACATACATA TATATTTTTG GGGGGGGGCG GACAGGAGAT GGGTGCAACT 2851 CCCTCCCATC CTACTCTCAC AGAAGGGCCT GGATGCAAGG TTACCCTTGA 2901 GCTGTGTGCC ACAGTCTGGT GCCCAGTCTG GCATGCAGCT ACCCAGGCCC 2951 ACCCATCACG TGTGATTGAC ATGTAGGTAC CCTGCCACGG CCTATGCCCC 3001 ACCTGCCCTG CTTCCTGGCT CCTTATCAGT GCCATGAGGG CAGAGGTGCT 3051 ACCTGGCCTT CCTGCCAGGA GCTCTCCACC CACTCACATT CCGTCCCCGC 3101 CGCCTCACTG CAGCCAGCGT GGCCCTAGGA CAGGAGGAGC TTCGGGCCCA 3151 GCTTCACCCT GCGGTGGGGC TGAGGGGTGG CCATCTCCTG CCCTGGGGCC 3201 ACTGGCTTCA CATTCTGGGC TGACTCATAG GGGAGTAGGG GTGGAGTCAC 3251 CAAAACCAGT GCTGGGACAA AGATGGGGAA GGTGTGTGAA CTTTTTAAAA 3301 TAAACACAAA AACACAGGAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 3351 AAG BLAST Results Entry HS1042K10 from database EMBL: Human DNA sequence from clone 1042K10 on chromosome 22ql3.1-13.2. Contains the ADSL gene for Adenylosuccmate lyase (EC 4.3.2.2, Adenylosuccinase, ASL) and 4 novel genes (one with probable rabGAP domains and Src homology domain 3) . Contains ESTs, STSs, GSSs and a putative CpG island. Score = 7997, P = O.Oe+00, identities = 1617/1645 7 exons Medline entries No Medline entry Peptide information for frame 3 ORF from 183 bp to 2213 bp; peptide length: 677 Category: similarity to unknown protein Classification: unclassified 1 MDSSYAKILQ QQQLFLQLQI LNQQQQQHHN YQAILPAPPK SAGEALGSSG 51 TPPVRSLΞTT NSSSSSGAPG PCGLARQNST SLTGKPGALP ANLDDMKVAE 101 LKQELKLRSL PVSGTKTELI ERLRAYQDQI SPVPGAPKAP AATSILHKAG 151 EVVVAFPAAR LSTGPALVAA GLAPAEVVVA TVASSGVVKF GSTGSTPPVS 201 PTPSERSLLS TGDENSTPGD TFGEMVTSPL TQLTLQASPL QILVKEEGPR 251 AGSCCLSPGG RAELEGRDKD QMLQEKDKQI EALTRMLRQK QQLVERLKLQ 301 LEQEKRAQQP APAPAPLGTP VKQENSFSSC QLSQQPLGPA HPFNPSLAAP 351 ATNHIDPCAV APGPPSVVVK QEALQPEPEP VPAPQLLLGP QGPGLIKGVA 401 PPTLITDSTG THLVLTVTNK NADSPGLSSG SPQQPSSQPG SPAPAPSAQM 451 DLEHPLQPLF GTPTΞLLKKE PPGYEEAMSQ QPKQQENGSS SQQMDDLFDI 501 LIQSGEISAD FKEPPSLPGK EKPSPKTVCG SPLAAQPΞPS AELPQAAPPP 551 PGSPSLPGRL EDFLESSTGL PLLTSGHDGP EPLSLIDDLH SQMLSSTAIL 601 DHPPSPMDTS ELHFVPEPSS TMGLDLADGH LDSMDWLELS SGGPVLSLAP 651 LSTTAPSLFS TDFLDGHDLQ LHWDSCL BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_22nl3, frame 3 TREMBL:HS1042K10_6 gene: "dJ1042K10.3"; product: "dJ1042K10.3 (novel protein)"; Human DNA sequence from clone 1042K10 on chromosome 22ql3.1-13.2. Contains the ADSL gene for Adenylosuccmate lyase (EC 4.3.2.2, Adenylosuccinase, ASL) and 4 novel genes (one with probable rabGAP domains and Src homology domain 3) . Contains ESTs, STSs, GSSs and a putative CpG island., N = 1, Score = 1285, P = 4.9e-131 TREMBL :CEUK06A9_3 gene: "K06A9.1a"; Caenorhabditis elegans cosmid K06A9., N = 2, Score = 149, P = 1.3e-09 TREMBLNEW: SSI132828_1 product: "p210 protein"; Spermatozopsis similis mRNA for p210 protein, partial, N = 1, Score = 171, P = 2.8e-09 >TREMBL:HS1042K10_6 gene: "dJ1042κl0.3"; product: "dJ1042K10.3 (novel protein)"; Human DNA sequence from clone 1042K10 on chromosome 22ql3.1-13.2. Contains the ADSL gene for Adenylosuccmate lyase (EC 4.3.2.2, Adenylosuccinase, ASL) and 4 novel genes (one with probable rabGAP domains and Src homology domain 3) . Contains ESTs, STSs, GSSs and a putative CpG island. Length = 243 HSPs: Score = 1285 (192.8 bits), Expect = 4.9e-131, P = 4.9e-131 Identities = 243/243 (100%), Positives = 243/243 (100%) Query: 435 PSSQPGΞPAPAPSAQMDLEHPLQPLFGTPTSLLKKEPPGYEEAMSQQPKQQENGSSSQQM 494 PΞSQPGSPAPAPSAQMDLEHPLQPLFGTPTSLLKKEPPGYEEAMSQQPKQQENGSSSQQM Sbjct: 1 PSSQPGΞPAPAPSAQMDLEHPLQPLFGTPTSLLKKEPPGYEEAMΞQQPKQQENGΞSSQQM 60 Query: 495 DDLFDILIQΞGEISADFKEPPSLPGKEKPSPKTVCGSPLAAQPSPSAELPQAAPPPPGΞP 554 DDLFDILIQSGEISADFKEPPSLPGKEKPSPKTVCGSPLAAQPSPSAELPQAAPPPPGSP Sbjct: 61 DDLFDILIQSGEISADFKEPPSLPGKEKPSPKTVCGSPLAAQPSPSAELPQAAPPPPGSP 120 Query: 555 SLPGRLEDFLESSTGLPLLTSGHDGPEPLSLIDDLHSQMLSSTAILDHPPSPMDTSELHF 614 SLPGRLEDFLESSTGLPLLTSGHDGPEPLSLIDDLHΞQMLSSTAILDHPPSPMDTSELHF Sbjct: 121 SLPGRLEDFLESSTGLPLLTSGHDGPEPLSLIDDLHSQMLSSTAILDHPPSPMDTSELHF 180 Query: 615 VPEPSSTMGLDLADGHLDSMDWLELSSGGPVLSLAPLSTTAPSLFSTDFLDGHDLQLHWD 674 VPEPSSTMGLDLADGHLDSMDWLELSSGGPVLSLAPLSTTAPSLFSTDFLDGHDLQLHWD Sbjct: 181 VPEPSSTMGLDLADGHLDSMDWLELSSGGPVLSLAPLSTTAPSLFSTDFLDGHDLQLHWD 240 Query: 675 SCL 677 SCL Sbjct: 241 SCL 243 Pedant information for DKFZphtes3_22nl3, frame 3 Report for DKFZphtes3_22nl3.3 [LENGTH] 677 [MW] 70743.01 [pi] 4.93 [HOMOL] TREMBL :HS1042K10_6 gene: "dJ1042K10.3"; product: "dJ1042K10.3 (novel protein)' Human DNA sequence from clone 1042K10 on chromosome 22ql3.1-13.2. Contains the ADSL gene for Adenylosuccmate lyase (EC 4.3.2.2, Adenylosuccinase, ASL) and 4 novel genes (one with probable rabGAP domains and Src homology domain 3) . Contains ESTs, STSs, GSSs and a putative CpG island, le-111 [KW] TRANSMEMBRANE 1 [KW] LOW_COMPLEXITY 21.57 % [KW] COILED COIL 4.58 % SEQ MDSSYAKILQQQQLFLQLQILNQQQQQHHNYQAILPAPPKSAGEALGSSGTPPVRSLSTT SEG xxxxxxxxxxxxxxxxxxx xxxxx PRD ccchhhhhhhhhhhhhhhhhhhhhhhhcceeeeeecccccceeeecccccccceeecccc COILS MEM SEQ NSSSSSGAPGPCGLARQNSTSLTGKPGALPANLDDMKVAELKQELKLRSLPVSGTKTELI SEG xxxxx PRD cccccccccccceeecccccccccccccccccccchhhhhhhhhhhhhhcccccchhhhh COILS MEM SEQ ERLRAYQDQISPVPGAPKAPAATSILHKAGEVVVAFPAARLSTGPALVAAGLAPAEVVVA SEG xxxxxxxxxxxxxxxxx PRD hhhhhhhhhcccccccccccceeeeeeeccceeeeccccccccccccccccccceeeeee COILS MEM . MMMMMMMMMMMMMMMMMMMMMM SEQ TVASSGVVKFGSTGSTPPVSPTPSERSLLSTGDENSTPGDTFGEMVTSPLTQLTLQASPL SEG xxxxxxx .. xxxxxxxxxxxxxx PRD eeecccccccccccccccccccccceeeeccccccccccccccceeecccceeeecccce COILS MEM M. SEQ QILVKEEGPRAGSCCLSPGGRAELEGRDKDQMLQEKDKQIEALTRMLRQKQQLVERLKLQ SEG PRD eeeeeccccccccccccccccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCC MEM SEQ LEQEKRAQQPAPAPAPLGTPVKQENSFSSCQLSQQPLGPAHPFNPSLAAPATNHIDPCAV SEG xxxxxxxxxx PRD hhhhhhhhhcccccccccccccccccceeeeecccccccccccccceeeccccccccccc COILS ccccccc MEM SEQ APGPPSVVVKQEALQPEPEPVPAPQLLLGPQGPGLIKGVAPPTLITDSTGTHLVLTVTNK SEG xxxxxxxxxxxx PRD cccccceeeeeccccccccccccceeeccccccceeeeecccccccccccceeeeeeecc COILS MEM SEQ NADSPGLSSGSPQQPSSQPGSPAPAPSAQMDLEHPLQPLFGTPTSLLKKEPPGYEEAMSQ SEG ... xxxxxxxxxxxxxxxxxxxxxxxxxx PRD ccccccccccccccccccccccccccchhhhhhhhhcccccccccccccccccccccccc COILS MEM SEQ QPKQQENGSSSQQMDDLFDILIQSGEISADFKEPPSLPGKEKPSPKTVCGSPLAAQPSPS SEG xxxxxxxxxxx PRD ccccccccccccchhhhhhhhhcccccccccccccccccccccccccccccccccccccc COILS MEM SEQ AELPQAAPPPPGSPSLPGRLEDFLESSTGLPLLTSGHDGPEPLSLIDDLHSQMLSSTAIL SEG xxxxxxxxxxxxxxxxxx PRD ccccccccccccccccccccccccccccccccccccccccccccchhhhhhhhcccceee COILS MEM SEQ DHPPSPMDTSELHFVPEPSSTMGLDLADGHLDSMDWLELSSGGPVLSLAPLSTTAPSLFS SEG PRD ccccccccccccccccccccccccccccccccccceeeeccccceeeeeecccccccccc COILS MEM SEQ TDFLDGHDLQLHWDSCL SEG PRD cccccccceeecccccc COILS MEM (No Prosite data available for DKFZphtes3_22nl3.3) (No Pfam data available for DKFZphtes3_22nl3.3) DKFZphtes3 23111 group: intracellular transport and trafficking DKFZphtes3_23111 encodes a novel 186 amino acid protein nearly identical to mouse ADP- ribosylation-like factor homolog 6 (Arl6) . Protein secretion through the endoplasmic reticulum and the Golgi vesicular trafficking system is initiated by the binding of ADP-ribosylation factors (ARFs) to donor membranes, leading to recruitment of cocatomer, bud formation, and eventual vesicle release. ARFs are approximately 20-kDa GTPases that are active with bound GTP and inactive with GDP bound. The novel protein contains an ATP/GTP-bmding site motif A (P-loop) and seems to be a novel ARF. It seems to have an important role in vesicular transport and vesicular trafficking. The new protein can find application in modulating vesicle transport and trafficking in cells. nearly identical to mouse Arl6, ADP-ribosylation-like factor homolog start at Bp 15 matches kozak consensus ANNatgG Sequenced by LMU Locus : unknown Insert length: 717 bp Poly A stretch at pos. 689, no polyadenylation signal found 1 ATTTGAATCA CATTATGGGA TTGCTAGACA GACTTTCAGT CTTGCTTGGC 51 CTGAAGAAGA AGGAGGTTCA TGTTTTGTGC CTTGGGCTAG ATAATAGTGG 101 CAAAACGACG ATCATTAACA AACTTAAACC TTCAAATGCT CAATCTCAAA 151 ATATCCTTCC AACAATAGGA TTCAGCATAG AGAAATTCAA ATCATCCAGT 201 TTGTCATTTA CAGTGTTTGA CATGTCAGGT CAAGGAAGAT ACAGAAATCT 251 CTGGGAACAC TATTATAAAG AAGGCCAAGC TATTATTTTT GTCATTGATA 301 GTAGTGATAG ATTAAGAATG GTTGTGGCCA AAGAAGAACT CGATACTCTT 351 CTGAATCATC CAGATATTAA ACACCGTCGA ATTCCAATCT TATTCTTTGC 401 AAATAAAATG GATCTTAGAG ATGCAGTGAC ATCTGTAAAA GTGTCTCAGT 451 TGCTGTGTTT AGAGAACATC AAAGATAAAC CCTGGCATAT TTGTGCTAGT 501 GATGCCATAA AAGGAGAAGG CTTGCAAGAA GGTGTAGACT GGCTTCAAGA 551 TCAGATCCAG ACTGTGAAGA CATGAAAAGA TAATAGTTGG AAACCTCAGC 601 AATTTTCAAT TCAAGGAATC TATCTAAGAC AAATAGAATA CATTTTGTAA 651 AAGATGTTTA TGCATCAAAA AATATAATTT TCTGCTTGCA AAAAAAAAAA 701 AAAAAAAAAA AAAAAAG BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 3 ORF from 15 bp to 572 bp; peptide length: 186 Category: strong similarity to known protein Classification: Intacellular transport and traffic Prosite motifs: ATP GTP A (24-32) 1 MGLLDRLSVL LGLKKKEVHV LCLGLDNSGK TTIINKLKPS NAQSQNILPT 51 IGFSIEKFKS SSLSFTVFDM SGQGRYRNLW EHYYKEGQAI IFVIDSSDRL 101 RMVVAKEELD TLLNHPDIKH RRIPILFFAN KMDLRDAVTS VKVSQLLCLE 151 NIKDKPWHIC ASDAIKGEGL QEGVDWLQDQ IQTVKT BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_23111, frame 3 TREMBL:AF031903_1 gene: "Arl6"; product: "ADP-ribosylation-like factor homolog ARL6"; Mus musculus ADP-ribosylation-like factor homolog ARL6 (Arl6) mRNA, complete eds., N = 1, Score = 923, P = l.le-92 TREMBL :CEC38D4_5 gene: "C38D4.8"; Caenorhabditis elegans cosmid C38D4, N = 1, Score = 418, P = 3.6e-39 PIR:Ξ66337 ADP-ribosylation factor 1 - Chlamydomonas reinhardtn, N = 1, Score = 373, P = 2. le-34 SWISSPROT :ARF1_CHLRE ADP-RIBOSYLATION FACTOR 1., N = 1, Score = 372, P = 2.7e-34 >TREMBL:AF031903_1 gene: "Arl6"; product: "ADP-ribosylation-like factor homolog ARL6"; Mus musculus ADP-ribosylation-like factor homolog ARL6 (Arl6) mRNA, complete eds. Length = 186 HSPs: Score = 923 (138.5 bits). Expect = l.le-92, P = l.le-92 Identities = 178/186 (95%), Positives = 184/186 (98%) Query: 1 MGLLDRLSVLLGLKKKEVHVLCLGLDNSGKTTIINKLKPSNAQSQNILPTIGFSIEKFKS 60 MGLLDRLS LLGLKKKEVHVLCLGLDNSGKTTIINKLKPSNAQSQ+I+PTIGFSIEKFKS Sbjct: 1 MGLLDRLSGLLGLKKKEVHVLCLGLDNSGKTTIINKLKPSNAQSQDIVPTIGFSIEKFKS 60 Query: 61 SSLSFTVFDMSGQGRYRNLWEHYYKEGQAIIFVIDSSDRLRMVVAKEELDTLLNHPDIKH 120 SSLSFTVFDMSGQGRYRNLWEHYYK+GQAIIFVIDSSD+LRMVVAKEELDTLLNHPDIKH Sbjct: 61 SSLSFTVFDMSGQGRYRNLWEHYYKDGQAIIFVIDSSDKLRMVVAKEELDTLLNHPDIKH 120 Query: 121 RRIPILFFANKMDLRDAVTSVKVSQLLCLENIKDKPWHICAΞDAIKGEGLQEGVDWLQDQ 180 RRIPILFFANKMDLRD+VTSVKVΞQLLCLE+IKDKPWHICASDAIKGEGLQEGVDWLQDQ Sbjct: 121 RRIPILFFANKMDLRDSVTSVKVSQLLCLESIKDKPWHICASDAIKGEGLQEGVDWLQDQ 180 Query: 181 IQTVKT 186 IQ VKT Sbjct: 181 IQAVKT 186 Pedant information for DKFZphtes3_23111, frame 3 Report for DKFZphtes3_23111.3 [LENGTH] 186 [MW] 21097.69 [pi] 8.72 [HOMOL] TREMBL :AF031903_1 gene: "Arl6"; product: "ADP-ribosylation-like factor homolog ARL6"; Mus musculus ADP-ribosylation-like factor homolog ARL6 (Arl6) mRNA, complete eds. 4e-94 [FUNCAT] 30.08 organization of golgi [S. cerevisiae, YDL192w] le-36 [FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YDL192w] le-36 [FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YDL192w] le-36 [FUNCAT] 30.09 organization of intracellular transport vesicles [S. cerevisiae, YDL137w] 2e-36 [FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylation, farnesylation and processing) S. cerevisiae, YBR164c] 2e-32 [FUNCAT] 30.03 organization of cytoplasm S. cerevisiae, YBR164C] 2e-32 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YMR138w] 4e-19 [FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YMR138w] 4e-19 [FUNCAT] r general function prediction [M. jannaschn MJ1339] 2e-05 [FUNCAT] 30.02 organization of plasma membrane [S. cerevisiae, YHR005c] 4e-05 [FUNCAT] 03.07 pheromone response, matmg-type determination, sex-specific proteins [S. cerevisiae, YHR005c] 4e-05 [FUNCAT] 10.05.07 g-proteins [S. cerevisiae, YHR005C] 4e-05 [FUNCAT] 08.13 vacuolar transport [S. cerevisiae, YKR014c] 2e-04 [FUNCAT] 08.19 cellular import [S. cerevisiae, YKR014c] 2e-04 [FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YKR014c] 2e-04 [FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YFL005w] 4e-04 [BLOCKS] BL01288C [BLOCKS] BL01020C SARI family proteins [BLOCKS] BL01019C ADP-ribosylation factors family proteins [BLOCKS] BL01019B ADP-ribosylation factors family proteins [BLOCKS] BL01019A ADP-ribosylation factors family proteins [SCOP] dlas3_2 3.29.1.4.12 Transduem (alpha subunit), insertion domai 2e-45 [SCOP] dlmhl 3.29.1.4.2 Racl [Human (Homo sapiens) 2e-46 [SCOP] d5p 2 l 3.29.1.4.1 cH-p21 Ras protein [human (Homo sapiens) 5e-37 [SCOP] dlhura_ 3.29.1.4.8 ADP-ribosylation factor 1 (ARFl) [human (Hom 4e-61 [SCOP] dla2kc_ 3.29.1.4.5 Ran Nuclear transport factor-2 (NTF2) [Do 4e-33 [PIRKW] glycoprotein 2e-33 [PIRKW] monomer 3e-31 [PIRKW] P-loop 2e-35 [PIRKW] lipoprotein 2e-33 [PIRKW] GTP binding 2e-35 [SUPFAM] ADP-ribosylation factor 2e-35 [PROSITE] ATP_GTP_A 1 [PFAM] ADP-ribosylation factors (Arf family) (contains ATP/GTP binding P-loop) [KW] Alpha_Beta [KW] 3D [KW] LOW COMPLEXITY 5.91 % SEQ MGLLDRLSVLLGLKKKEVHVLCLGLDNSGKTTIINKLKPSNAQSQNILPTIGFSIEKFKS SEG .. xxxxxxxxxxx lhurA CCCCEEEEEETTTTCHHHHHHHHCCCCEEEE—EEETTEEEEEEEE SEQ SSLSFTVFDMSGQGRYRNLWEHYYKEGQAIIFVIDSSDRLRMVVAKEELDTLLNHPDIKH SEG lhurA TTEEEEEEETTTTTTTCCCHHHHHHCEEEEEEEEETTTTTHHHHHHHHHHHHHHTTTT— SEQ RRIPILFFANKMDLRDAVTSVKVSQLLCLENIKDKPWHICASDAIKGEGLQEGVDWLQDQ SEG lhurA TTTEEEEEEETTTTTTTCCHHHHHHHHCGGGTTTTCEEEEECBTTTTBTHHHHHHHHHHH SEQ IQTVKT SEG lhurA HHHHC. Prosite for DKFZphtes3_23111.3 PS00017 24->32 ATP GTP A PDOC00017 Pfam for DKFZphtes3_23111.3 HMM_NAME ADP-ribosylation factors (Arf family) (contains ATP/GTP binding P-loop) HMM *GMgWfsIFrkMWGlWNKEMRILMLGLDNAGKTTILYMLKlgE.. IVTTI MG++ ++ ++GL +KE+++L LGLDN+GKTTI+++LK+ ++ Query 1 -MGLLDRLSVLLGLKKKEVHVLCLGLDNSGKTTIINKLKPSNAQSQNIL 48 HMM PTIGFNVETVeYKNIKFNVWDVGGQdsIRPYWRHYYpNTDGIIWVVDSaD PTIGF +E+ + ++F+V+D GQ + R +W HYY + ++II+V+DS+D Query 49 PTIGFSIEKFKSSSLSFTVFDMSGQGRYRNLWEHYYKEGQAIIFVIDSSD 98 HMM RDRMeEaKqELHaMLNEEEL.. rDAPlLIFANKQDLPgAMSesEIREaLG R RM AK+EL+ +LN+ ++ R+ P+L FANK DL++A+++ +++ +L Query 99 RLRMVVAKEELDTLLNHPDIKHRRIPILFFANKMDLRDAVTSVKVSQLLC 148 HMM LHelRCnRPWYIQMCCAVtGEGLYEGMDWLSNYInkRkK* L++I+ + PW+I +++A++GEGL+EG DWL ++I+ K Query 149 LENIK-DKPWHICASDAIKGEGLQEGVDWLQDQIQTVKT 186 DKFZphtes3_23nl9 group: testes derived DKFZphtes3_23nl9 encodes a novel 387 amino acid protein with similarity to rat protein kinase C-mteracting RBCC protein 1. The novel protein contains not the RING-B box-coiled coil (RBCC) motif of RBCC protein 1, and thus is not a member of this subgroup of RING finger proteins. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . similarity to rat protein kinase C-interacting RBCC protein 1 start at Bp 209 matches kozak consensus PyNNatgG similarity to of C-termmal part to N-termmus of RBCK1 Sequenced by LMU Locus : unknown Insert length: 1579 bp Poly A stretch at pos. 1535, polyadenylation signal at pos. 1515 1 CGGAGACCCT CGGGCCGTGT CCATTTGTGG GCAAAGCCAG CGGGGCAGGC 51 TTGGCCAGAG TGCACCACTC GGCGCCGTCC CAGGCCCGAC GCTCTGGGCG 101 CGCCCGGAAC CCCAGGTTCG CGGCCCGTGT TTCCGACCGG CGGAGGGGGC 151 TCAGCGGCCC GATCCCACGG AAGCGCGCTC GGAGGGGTGG GACCCGGCCG 201 GACCGGAGAT GGCGCCGCCA GCGGGCGGGG CGGCGGCGGC GGCCTCGGAC 251 TTGGGCTCCG CCGCAGTGCT CTTGGCTGTG CACGCCGCGG TGAGGCCGCT 301 GGGCGCCGGG CCAGACGCCG AGGCACAGCT GCGGAGGCTG CAGCTGAGCG 351 CGGACCCTGA GAGGCCTGGG CGCTTCCGGC TGGAGCTGCT GGGCGCGGGA 401 CCTGGGGCGG TTAATTTGGA GTGGCCCCTG GAGTCAGTTT CCTACACCAT 451 CCGAGGCCCC ACCCAGCACG AGCTACAGCC TCCACCAGGA GGGCCTGGAA 501 CCCTCAGCCT GCACTTCCTC AACCCTCAGG AAGCTCAGCG GTGGGCAGTC 551 CTAGTCCGAG GTGCCACCGT GGAAGGACAG AATGGCAGCA AGAGCAACTC 601 ACCACCAGCC TTGGGCCCAG AAGCATGCCC TGTCTCCCTG CCCAGTCCCC 651 CGGAAGCCTC CACACTCAAG GGCCCTCCAC CTGAGGCAGA TCTTCCTAGG 701 AGCCCTGGAA ACTTGACGGA GAGAGAAGAG CTGGCAGGGA GCCTGGCCCG 751 GGCTATTGCA GGTGGAGACG AGAAGGGGGC AGCCCAAGTG GCAGCCGTCC 801 TGGCCCAGCA TCGTGTGGCC CTGAGTGTTC AGCTTCAGGA GGCCTGCTTC 851 CCACCTGGCC CCATCAGGCT GCAGGTCACA CTTGAAGACG CTGCCTCTGC 901 CGCATCCGCC GCGTCCTCTG CACACGTTGC CCTGCAGGTC CACCCCCACT 951 GCACTGTTGC AGCTCTCCAG GAGCAGGTGT TCTCAGAGCT CGGTTTCCCG 1001 CCAGCCGTGC AACGCTGGGT CATCGGACGG TGCCTGTGTG TGCCTGAGCG 1051 CAGCCTTGCC TCTTACGGGG TTCGGCAGGA TGGGGACCCT GCTTTCCTCT 1101 ACTTGCTGTC AGCTCCTCGA GAAGCCCCAG CCACAGGACC TAGCCCTCAG 1151 CACCCCCAGA AGATGGACGG GGAACTTGGA CGCTTGTTTC CCCCATCATT 1201 GGGGCTACCC CCAGGCCCCC AGCCAGCTGC CTCCAGCCTG CCCAGTCCAC 1251 TCCAGCCCAG CTGGTCCTGT CCTTCCTGCA CCTTCATCAA TGCCCCAGAC 1301 CGCCCTGGCT GTGAGATGTG TAGCACCCAG AGGCCCTGCA CTTGGGACCC 1351 CCTTGCTGCA GCTTCCACCT AGCAGCCACC AGAGGTTACA AGGGGAGAGT 1401 GGCCCTTCCC TCACAAGTCC GACATCTCCA GGCCCCCACT GAACTCCGGG 1451 GACCTCTACT GACTGCTTGC TGGGACAGTC ACCAGGGTTG GGGGGAAGGG 1501 CCACAAAATG AAACCATTAA AGACCCTTAA GAGCCAAAAA AAAAAAAAAA 1551 AAAAAAAAAA AAAAAAAAAA AAAAAAAAG BLAST Results No BLAST result Medlme entries No Medl e entry Peptide information for frame 2 ORF from 209 bp to 1369 bp; peptide length: 387 Category: similarity to known protein Classification: Cell signaling/communication 1 MAPPAGGAAA AASDLGSAAV LLAVHAAVRP LGAGPDAEAQ LRRLQLSADP 51 ERPGRFRLEL LGAGPGAVNL EWPLESVSYT IRGPTQHELQ PPPGGPGTLS 101 LHFLNPQEAQ RWAVLVRGAT VEGQNGSKSN SPPALGPEAC PVΞLPSPPEA 151 STLKGPPPEA DLPRSPGNLT EREELAGSLA RAIAGGDEKG AAQVAAVLAQ 201 HRVALSVQLQ EACFPPGPIR LQVTLEDAAS AASAASSAHV ALQVHPHCTV 251 AALQEQVFSE LGFPPAVQRW VIGRCLCVPE RSLASYGVRQ DGDPAFLYLL 301 ΞAPREAPATG PSPQHPQKMD GELGRLFPPS LGLPPGPQPA ASSLPSPLQP 351 SWSCPSCTFI NAPDRPGCEM CSTQRPCTWD PLAAAST BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_23nl9, frame 2 PIR:JC5983 protein kinase C-interacting RBCC protein 1 - rat, N = 1, Score = 353, P = 2.8e-32 TREMBL :AB011369_1 product: "RBCK2"; Rattus norvegicus mRNA for RBCK2, complete eds., N = 1, Score = 353, P = 2.8e-32 TREMBL :U67322_1 gene: "XAP4"; product: "HBV associated factor"; Human HBV associated factor (XAP4) mRNA, complete eds., N = 1, Score = 286, P = 8.5e-25 TREMBLNEW:AF124663_1 product: "UbcM4 interacting protein 28"; Mus musculus UbcM4 interacting protein 28 mRNA, complete eds., N = 1, Score = 367, P = 9.3e-34 >TREMBLNEW: AF124663_1 product: "UbcM4 interacting protein 28"; Mus musculus UbcM4 interacting protein 28 mRNA, complete eds. Length = 498 HSPs: Score = 367 (55.1 bits), Expect = 9.3e-34, P = 9.3e-34 Identities = 95/212 (44%), Positives = 129/212 (60%) Query: 175 LAGSLARAIAGGDEKGAAQVAAVLAQHRVALSVQLQEACFPPGPIRLQVTLEDAASAASA 234 +A SLARA+AGGDE+ A + A LA+ RV L VQ++ P IRL V++EDA Sbjct: 1 MALSLARAVAGGDEQAAIKYATWLAEQRVPLRVQVKPEVSPTQDIRLCVSVEDAYM 56 Query: 235 ASSAHVALQVHPHCTVAALQEQVFSELGFPPAVQRWVIGRCLCVPERSLASYGVRQDGDP 294 + + L V P TVA+L++ VF + GFPP++Q+WV+G+ L + +L S+G+R++GD Sbjct: 57 -HTVTIWLTVRPDMTVASLKDMVFLDYGFPPSLQQWVVGQRLARDQETLHSHGIRRNGDG 115 Query: 295 AFLYLLSAPREAPATGPSPQHPQK MDGELG—RLFPPSLG-LPPG-PQPAASSLP 345 A+LYLLSA T +PQ Q+ M +LG L S G L P P+P + P Sbjct: 116 AYLYLLSARN TSLNPQELQRQRQLRMLEDLGFKDLTLQSRGPLEPVLPKPRTNQEP 171 Query: 346 SPLQP—ΞWSCPSCTFINAPDRPGCEMCSTQRPCTW 379 +P P W CP CTFIN P RPGCEMC RP T+ Sbjct: 172 GQPDAAPESPPVGWQCPGCTFINKPTRPGCEMCCRARPETY 212 Pedant information for DKFZphtes3_23nl9, frame 2 Report for DKFZphtes3_23nl9.2 [LENGTH] 387 [MW] 39949.29 [pi] 5.53 [HOMOL] TREMBLNEW:AF124663_1 product: "UbcM4 interacting protein 28"; Mus musculus UbcM4 interacting protein 28 mRNA, complete eds. le-22 [BLOCKS] BL00578B [KW] Alpha_Beta [KW] LOW_COMPLEXITY 17.57 % SEQ MAPPAGGAAAAASDLGΞAAVLLAVHAAVRPLGAGPDAEAQLRRLQLSADPERPGRFRLEL SEG . xxxxxxxxxxxxxxxxxxxxxxxxxxx PRD cccccchhhhhhhhhhhhhhhhhhhhhccccccccchhhhhhhhhhhccccccccceeee SEQ LGAGPGAVNLEWPLESVSYTIRGPTQHELQPPPGGPGTLSLHFLNPQEAQRWAVLVRGAT SEG PRD ccccccceeecccceeeeeeccccccccccccccccceeeeeecccchhhhhheeeecce SEQ VEGQNGSKSNSPPALGPEACPVSLPSPPEASTLKGPPPEADLPRSPGNLTEREELAGSLA SEG PRD eecccccccccccccccccccccccccccccccccccccccccccccchhhhhhhhhhhh SEQ RAIAGGDEKGAAQVAAVLAQHRVALSVQLQEACFPPGPIRLQVTLEDAASAASAASSAHV SEG xxxxxxxxxxx .. PRD hhhhcccchhhhhhhhhhhhhhhhhhccccccccccccceeeccchhhhhhhhhhhhhee SEQ ALQVHPHCTVAALQEQVFSELGFPPAVQRWVIGRCLCVPERSLASYGVRQDGDPAFLYLL SEG PRD eeeccccchhhhhhhhhhhhccccccchhhhhhhhhhhhccccccccccccccceeeeec SEQ SAPREAPATGPSPQHPQKMDGELGRLFPPSLGLPPGPQPAASSLPSPLQPSWSCPSCTFI SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxx... PRD cccccccccchhhhhhhhhhhhccccccccccccccccccccccccccccccccccceee SEQ NAPDRPGCEMCSTQRPCTWDPLAAAST SEG PRD ccccccccccccccccccccceeeccc (No Prosite data available for DKFZphtes3_23nl9.2) (No Pfam data available for DKFZphtes3_23nl9.2) similarity to rat protein kinase C-mteracting RBCC protein 1 start at Bp 209 matches kozak consensus PyNNatgG similarity to of C-termmal part to N-terminus of RBCK1 Sequenced by LMU Locus: unknown Insert length: 1579 bp Poly A stretch at pos. 1535, polyadenylation signal at pos. 1515 1 CGGAGACCCT CGGGCCGTGT CCATTTGTGG GCAAAGCCAG CGGGGCAGGC 51 TTGGCCAGAG TGCACCACTC GGCGCCGTCC CAGGCCCGAC GCTCTGGGCG 101 CGCCCGGAAC CCCAGGTTCG CGGCCCGTGT TTCCGACCGG CGGAGGGGGC 151 TCAGCGGCCC GATCCCACGG AAGCGCGCTC GGAGGGGTGG GACCCGGCCG 201 GACCGGAGAT GGCGCCGCCA GCGGGCGGGG CGGCGGCGGC GGCCTCGGAC 251 TTGGGCTCCG CCGCAGTGCT CTTGGCTGTG CACGCCGCGG TGAGGCCGCT 301 GGGCGCCGGG CCAGACGCCG AGGCACAGCT GCGGAGGCTG CAGCTGAGCG 351 CGGACCCTGA GAGGCCTGGG CGCTTCCGGC TGGAGCTGCT GGGCGCGGGA 401 CCTGGGGCGG TTAATTTGGA GTGGCCCCTG GAGTCAGTTT CCTACACCAT 451 CCGAGGCCCC ACCCAGCACG AGCTACAGCC TCCACCAGGA GGGCCTGGAA 501 CCCTCAGCCT GCACTTCCTC AACCCTCAGG AAGCTCAGCG GTGGGCAGTC 551 CTAGTCCGAG GTGCCACCGT GGAAGGACAG AATGGCAGCA AGAGCAACTC 601 ACCACCAGCC TTGGGCCCAG AAGCATGCCC TGTCTCCCTG CCCAGTCCCC 651 CGGAAGCCTC CACACTCAAG GGCCCTCCAC CTGAGGCAGA TCTTCCTAGG 701 AGCCCTGGAA ACTTGACGGA GAGAGAAGAG CTGGCAGGGA GCCTGGCCCG 751 GGCTATTGCA GGTGGAGACG AGAAGGGGGC AGCCCAAGTG GCAGCCGTCC 801 TGGCCCAGCA TCGTGTGGCC CTGAGTGTTC AGCTTCAGGA GGCCTGCTTC 851 CCACCTGGCC CCATCAGGCT GCAGGTCACA CTTGAAGACG CTGCCTCTGC 901 CGCATCCGCC GCGTCCTCTG CACACGTTGC CCTGCAGGTC CACCCCCACT 951 GCACTGTTGC AGCTCTCCAG GAGCAGGTGT TCTCAGAGCT CGGTTTCCCG 1001 CCAGCCGTGC AACGCTGGGT CATCGGACGG TGCCTGTGTG TGCCTGAGCG 1051 CAGCCTTGCC TCTTACGGGG TTCGGCAGGA TGGGGACCCT GCTTTCCTCT 1101 ACTTGCTGTC AGCTCCTCGA GAAGCCCCAG CCACAGGACC TAGCCCTCAG 1151 CACCCCCAGA AGATGGACGG GGAACTTGGA CGCTTGTTTC CCCCATCATT 1201 GGGGCTACCC CCAGGCCCCC AGCCAGCTGC CTCCAGCCTG CCCAGTCCAC 1251 TCCAGCCCAG CTGGTCCTGT CCTTCCTGCA CCTTCATCAA TGCCCCAGAC 1301 CGCCCTGGCT GTGAGATGTG TAGCACCCAG AGGCCCTGCA CTTGGGACCC 1351 CCTTGCTGCA GCTTCCACCT AGCAGCCACC AGAGGTTACA AGGGGAGAGT 1401 GGCCCTTCCC TCACAAGTCC GACATCTCCA GGCCCCCACT GAACTCCGGG 1451 GACCTCTACT GACTGCTTGC TGGGACAGTC ACCAGGGTTG GGGGGAAGGG 1501 CCACAAAATG AAACCATTAA AGACCCTTAA GAGCCAAAAA AAAAAAAAAA 1551 AAAAAAAAAA AAAAAAAAAA AAAAAAAAG BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 2 ORF from 209 bp to 1369 bp; peptide length: 387 Category: similarity to known protein Classification: Cell signaling/communication 1 MAPPAGGAAA AASDLGSAAV LLAVHAAVRP LGAGPDAEAQ LRRLQLSADP 51 ERPGRFRLEL LGAGPGAVNL EWPLESVSYT IRGPTQHELQ PPPGGPGTLS 101 LHFLNPQEAQ RWAVLVRGAT VEGQNGSKSN SPPALGPEAC PVSLPSPPEA 151 STLKGPPPEA DLPRSPGNLT EREELAGSLA RAIAGGDEKG AAQVAAVLAQ 201 HRVALSVQLQ EACFPPGPIR LQVTLEDAAS AASAASSAHV ALQVHPHCTV 251 AALQEQVFSE LGFPPAVQRW VIGRCLCVPE RSLASYGVRQ DGDPAFLYLL 301 SAPREAPATG PSPQHPQKMD GELGRLFPPS LGLPPGPQPA ASSLPSPLQP 351 SWSCPSCTFI NAPDRPGCEM CSTQRPCTWD PLAAAST BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_23nl9, frame 2 PIR:JC5983 protein kinase C-interactmg RBCC protein 1 - rat, N = 1, Score = 353, P = 2.8e-32 TREMBL :AB011369_1 product: "RBCK2"; Rattus norvegicus mRNA for RBCK2, complete eds., N = 1, Score = 353, P = 2.8e-32 TREMBL :U67322_1 gene: "XAP4"; product: "HBV associated factor"; Human HBV associated factor (XAP4) mRNA, complete eds., N = 1, Score = 286, P = 8.5e-25 TREMBLNEW:AF124663_1 product: "UbcM4 interacting protein 28"; Mus musculus UbcM4 interacting protein 28 mRNA, complete eds., N = 1, Score = 367, P = 9.3e-34 >TREMBLNEW:AF124663_1 product: "UbcM4 interacting protein 28"; Mus musculus UbcM4 interacting protein 28 mRNA, complete eds. Length = 498 HSPs: Score = 367 (55.1 bits), Expect = 9.3e-34, P = 9.3e-34 Identities = 95/212 (44%), Positives = 129/212 (60%) Query: 175 LAGSLARAIAGGDEKGAAQVAAVLAQHRVALSVQLQEACFPPGPIRLQVTLEDAASAASA 234 +A SLARA+AGGDE+ A + A LA+ RV L VQ++ P IRL V++EDA Sbjct: 1 MALSLARAVAGGDEQAAIKYATWLAEQRVPLRVQVKPEVSPTQDIRLCVSVEDAYM 56 Query: 235 ASSAHVALQVHPHCTVAALQEQVFSELGFPPAVQRWVIGRCLCVPERSLASYGVRQDGDP 294 + + L V P TVA+L++ VF + GFPP++Q+WV+G+ L + +L S+G+R++GD Sbjct: 57 -HTVTIWLTVRPDMTVASLKDMVFLDYGFPPSLQQWVVGQRLARDQETLHSHGIRRNGDG 115 Query: 295 AFLYLLSAPREAPATGPSPQHPQK MDGELG—RLFPPSLG-LPPG-PQPAASSLP 345 A+LYLLSA T +PQ Q+ M +LG L S G L P P+P + P Sbjct: 116 AYLYLLSARN TSLNPQELQRQRQLRMLEDLGFKDLTLQSRGPLEPVLPKPRTNQEP 171 Query: 346 SPLQP—SWSCPSCTFINAPDRPGCEMCSTQRPCTW 379 +P P W CP CTFIN P RPGCEMC RP T+ Sbjct: 172 GQPDAAPESPPVGWQCPGCTFINKPTRPGCEMCCRARPETY 212 Pedant information for DKFZphtes3_23nl9, frame 2 Report for DKFZphtes3_23nl9.2 [LENGTH] 387 [MW] 39949.29 [pi] 5.53 [HOMOL] TREMBLNEW:AF124663_1 product: "UbcM4 interacting protein 28"; Mus musculus UbcM4 interacting protein 28 mRNA, complete eds. le-22 [BLOCKS] BL00578B [KW] Alpha_Beta [KW] LOW_COMPLEXITY 17.57 % SEQ MAPPAGGAAAAAΞDLGSAAVLLAVHAAVRPLGAGPDAEAQLRRLQLSADPERPGRFRLEL SEG .xxxxxxxxxxxxxxxxxxxxxxxxxxx PRD cccccchhhhhhhhhhhhhhhhhhhhhccccccccchhhhhhhhhhhccccccccceeee SEQ LGAGPGAVNLEWPLESVSYTIRGPTQHELQPPPGGPGTLSLHFLNPQEAQRWAVLVRGAT SEG PRD ccccccceeecccceeeeeeccccccccccccccccceeeeeecccchhhhhheeeecce SEQ VEGQNGSKSNSPPALGPEACPVSLPSPPEASTLKGPPPEADLPRSPGNLTEREELAGSLA SEG PRD eecccccccccccccccccccccccccccccccccccccccccccccchhhhhhhhhhhh SEQ RAIAGGDEKGAAQVAAVLAQHRVALSVQLQEACFPPGPIRLQVTLEDAASAASAASSAHV SEG xxxxxxxxxxx .. PRD hhhhcccchhhhhhhhhhhhhhhhhhccccccccccccceeeccchhhhhhhhhhhhhee SEQ ALQVHPHCTVAALQEQVFSELGFPPAVQRWVIGRCLCVPERSLASYGVRQDGDPAFLYLL SEG PRD eeeccccchhhhhhhhhhhhccccccchhhhhhhhhhhhccccccccccccccceeeeec SEQ SAPREAPATGPSPQHPQKMDGELGRLFPPSLGLPPGPQPAASSLPSPLQPSWSCPSCTFI SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxx... PRD cccccccccchhhhhhhhhhhhccccccccccccccccccccccccccccccccccceee SEQ NAPDRPGCEMCSTQRPCTWDPLAAAST SEG PRD ccccccccccccccccccccceeeccc (No Prosite data available for DKFZphtes3_23nl9.2) (No Pfam data available for DKFZphtes3_23nl9.2) DKFZphtes3_26g22 group: intracellular transport/trafficking DKFZphtes3 26g22 encodes a novel 898 amino acid protein with similarity to kmesms. The novel protein contains a ATP/GTP-bindmg site motif A (P-loop) and a kinesin motor domain signature. Kinesm is a microtubule-associated force-producing protein that play a role in organelle transport. It is an oligomeric complex composed of two heavy chains and two light chains. The kinesm motor activity is directed toward the microtubule ' s plus end. The heavy chain contains a large globular N-terminal domain which is responsible for the motor activity of kinesin, which is known to hydrolyze ATP and to bind and move on microtubules. Several proteins involved in chromosome segregation and cell divsion contain this motor domain, such as drosophila claret segregational protein (ncd) , Drosophila kinesin-like protein (nod) , human CENP-E and human mitotic kines -like proteιn-1 (MKLP-1). The novel protein is a new kinesin like proptem. The new protein can find application in modulating chromosome transport in mitosis and meiosis and modulation of cell division. strong similarity to kinesins Sequenced by EMBL Locus : unknown Insert length: 3032 bp No poly A stretch found, no polyadenylation signal found 1 CTGAAGCGCT GGGAGGCGGA CATTAAAGTG AAGTGGTTGC GGTAACCTGG 51 CCTGGGCCTG AAGTGAGTGA GAGGCACATG AAGAGAAGTA TTCAAGTATT 101 TATACAGATA GGAATCAAGA TAATCAACAA TGTCTGTCAC TGAGGAAGAC 151 CTGTGCCACC ATATGAAAGT AGTAGTTCGT GTACGTCCGG AAAACACTAA 201 AGAAAAAGCA GCTGGATTTC ATAAAGTGGT TCATGTTGTG GATAAACATA 251 TCCTAGTTTT TGATCCCAAA CAAGAAGAAG TCAGTTTTTT CCATGGAAAG 301 AAAACTACAA ATCAAAATGT TATAAAGAAA CAAAATAAGG ATCTTAAATT 351 TGTATTTGAT GCTGTTTTTG ATGAAACGTC AACTCAGTCA GAAGTTTTTG 401 AACACACTAC TAAGCCAATT CTTCGTAGTT TTTTGAATGG ATATAATTGC 451 ACAGTACTTG CCTATGGTGC CACTGGTGCT GGGAAGACCC ACACTATGCT 501 AGGATCAGCT GATGAACCTG GAGTGATGTA TCTAACAATG TTACACCTTT 551 ACAAATGCAT GGATGAGATT AAAGAAGAGA AAATATGTAG TACTGCAGTT 601 TCATATCTGG AGGTATATAA TGAACAGATT CGTGATCTCT TAGTAAATTC 651 AGGGCCACTT GCTGTCCGGG AAGATACCCA AAAAGGGGTG GTCGTTCATG 701 GACTTACTTT ACACCAGCCC AAATCCTCAG AAGAAATTTT ACATTTATTG 751 GATAATGGAA ACAAAAACAG GACACAACAT CCCACTGATA TGAATGCCAC 801 ATCTTCTCGT TCTCATGCTG TTTTCCAAAT TTACTTGCGA CAACAAGACA 851 AAACAGCAAG TATCAATCAA AATGTCCGTA TTGCCAAGAT GTCACTCATT 901 GACCTGGCAG GATCTGAGCG AGCAAGTACT TCCGGTGCTA AGGGGACCCG 951 ATTTGTAGAA GGCACAAATA TTAATAGATC ACTTTTAGCT CTTGGGAATG 1001 TCATCAATGC CTTAGCAGAT TCAAAGAGAA AGAATCAGCA TATCCCTTAC 1051 AGAAATAGTA AGCTTACTCG CTTGTTAAAG GATTCTCTTG GAGGAAACTG 1101 TCAAACTATA ATGATAGCTG CTGTTAGTCC TTCCTCTGTA TTCTACGATG 1151 ACACATATAA CACTCTTAAG TATGCTAACC GGGCAAAGGA CATTAAATCT 1201 TCTTTGAAGA GCAATGTTCT TAATGTCAAT AATCATATAA CTCAATATGT 1251 AAAGATCTGT AATGAGCAGA AGGCAGAGAT TTTATTGTTA AAAGAAAAAC 1301 TAAAAGCCTA TGAAGAACAG AAAGCCTTCA CTAATGAAAA TGACCAAGCA 1351 AAGTTAATGA TTTCAAACCC TCAGGAAAAA GAAATCGAAA GGTTTCAAGA 1401 AATCCTGAAC TGCTTGTTCC AGAATCGAGA AGAAATTAGA CAAGAATATC 1451 TGAAGTTGGA AATGTTACTT AAAGAAAATG AACTTAAATC ATTCTACCAA 1501 CAACAGTGCC ATAAACAAAT AGAAATGATG TGTTCTGAAG ACAAAGTAGA 1551 AAAGGCCACT GGAAAACGAG ATCATAGACT TGCAATGTTG AAAACTCGTC 1601 GCTCCTACCT GGAGAAAAGG AGGGAGGAGG AATTGAAGCA ATTTGATGAG 1651 AATACTAATT GGCTCCATCG TGTCGAAAAA GAAATGGGAC TCTTAAGTCA 1701 AAACGGTCAT ATTCCAAAGG AACTCAAGAA AGATCTTCAT TGTCACCATT 1751 TGCACCTCCA GAACAAAGAT TTGAAAGCAC AAATTAGACA TATGATGGAT 1801 CTAGCTTGTC TTCAGGAACA GCAACACAGG CAGACTGAAG CAGTATTGAA 1851 TGCTTTACTT CCAACCCTAA GAAAACAATA TTGCACATTA AAAGAAGCCG 1901 GCCTGTCAAA TGCTGCTTTT GAATCTGACT TCAAAGAGAT CGAACATTTG 1951 GTAGAGAGGA AAAAAGTGGT AGTTTGGGCT GACCAAACTG CCGAACAACC 2001 AAAGCAAAAC GATCTACCAG GGATTTCTGT TCTTATGACC TTTCCACAAC 2051 TTGGACCAGT TCAGCCTATT CCTTGTTGCT CATCTTCAGG TGGAACTAAT 2101 CTGGTTAAGA TTCCTACAGA AAAAAGAACT CGGAGAAAAC TAATGCCATC 2151 TCCCTTGAAA GGACAGCATA CTCTAAAGTC TCCACCATCT CAAAGTGTGC 2201 AGCTCAATGA TTCTCTTAGC AAAGAACTTC AGCCTATTGT ATATACACCA 2251 GAAGACTGTA GAAAAGCTTT TCAAAATCCG TCTACAGTAA CCTTAATGAA 2301 ACCATCATCA TTTACTACAA GTTTTCAGGC TATCAGCTCA AACATAAACA 2351 GTGATAATTG TCTGAAAATG TTGTGTGAAG TAGCTATCCC TCATAATAGA 2401 AGAAAAGAAT GTGGACAGGA GGACTTGGAC TCTACATTTA CTATATGTGA 2451 AGACATCAAG AGCTCGAAGT GTAAATTACC CGAACAAGAA TCACTACCAA 2501 ATGATAACAA AGACATTTTA CAACGGCTTG ATCCTTCTTC ATTCTCAACT 2551 AAGCATTCTA TGCCTGTACC AAGCATGGTG CCATCCTACA TGGCAATGAC 2601 TACTGCTGCC AAAAGGAAAC GGAAATTAAC AAGTTCTACA TCAAACAGTT 2651 CGTTAACTGC AGACGTAAAT TCTGGATTTG CCAAACGTGT TCGACAAGAT 2701 AATTCAAGTG AGAAGCACTT ACAAGAAAAC AAACCAACAA TGGAACATAA 2751 AAGAAACATC TGTAAAATAA ATCCAAGCAT GGTTAGAAAA TTTGGAAGAA 2801 ATATTTCAAA AGGAAATCTA AGATAAATCA CTTCAAAACC AAGCAAAATG 2851 AAGTTGATCA AATCTGCTTT TCAAAGTTTA TCAATACCCT TTCAAAAATA 2901 TATTTAAAAT CTTTGAAAGA AGACCCATCT TAAAGCTAAG TTTACCCAAG 2951 TACTTTCAGC AAGCAGAAAA ATGAAACTCT TTGTTTTCTT CTTTTGTGTT 3001 CTAAAAAAAT AAAATTTCAA AAGAAAAAAA AA BLAST Results No BLAST result Medlme entries No Medline entry Peptide information for frame 1 ORF from 130 bp to 2823 bp; peptide length: 898 Category: strong similarity to known protein Classification: Cell structure/motility Prosite motifs: ATP_GTP_A (113-121) KINESIN MOTOR DOMAIN1 (252-264) 1 MSVTEEDLCH HMKVVVRVRP ENTKEKAAGF HKVVHVVDKH ILVFDPKQEE 51 VSFFHGKKTT NQNVIKKQNK DLKFVFDAVF DETSTQSEVF EHTTKPILRS 101 FLNGYNCTVL AYGATGAGKT HTMLGSADEP GVMYLTMLHL YKCMDEIKEE 151 KICSTAVSYL EVYNEQIRDL LVNSGPLAVR EDTQKGVVVH GLTLHQPKSS 201 EEILHLLDNG NKNRTQHPTD MNATSSRSHA VFQIYLRQQD KTASINQNVR 251 IAKMSLIDLA GSERASTSGA KGTRFVEGTN INRSLLALGN VINALADSKR 301 KNQHIPYRNS KLTRLLKDSL GGNCQTIMIA AVSPSSVFYD DTYNTLKYAN 351 RAKDIKSSLK SNVLNVNNHI TQYVKICNEQ KAEILLLKEK LKAYEEQKAF 401 TNENDQAKLM ISNPQEKEIE RFQEILNCLF QNREEIRQEY LKLEMLLKEN 451 ELKSFYQQQC HKQIEMMCSE DKVEKATGKR DHRLAMLKTR RSYLEKRREE 501 ELKQFDENTN WLHRVEKEMG LLΞQNGHIPK ELKKDLHCHH LHLQNKDLKA 551 QIRHMMDLAC LQEQQHRQTE AVLNALLPTL RKQYCTLKEA GLSNAAFESD 601 FKEIEHLVER KKVVVWADQT AEQPKQNDLP GISVLMTFPQ LGPVQPIPCC 651 SSSGGTNLVK IPTEKRTRRK LMPSPLKGQH TLKSPPSQSV QLNDSLSKEL 701 QPIVYTPEDC RKAFQNPSTV TLMKPSSFTT SFQAISSNIN SDNCLKMLCE 751 VAIPHNRRKE CGQEDLDSTF TICEDIKSSK CKLPEQESLP NDNKDILQRL 801 DPSSFSTKHS MPVPSMVPSY MAMTTAAKRK RKLTSSTSNΞ SLTADVNSGF 851 AKRVRQDNSS EKHLQENKPT MEHKRNICKI NPSMVRKFGR NISKGNLR BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_26g22, frame 1 SWISSPROT :YB3D_SCHPO PUTATIVE KINESIN-LIKE PROTEIN C2F12.13., N = 3, Score = 874, P = 9e-93 TREMBL :DMU89264_1 product: "kinesin like protein 67a"; Drosophila melanogaster kinesin like protein 67a mRNA, complete eds., N = 1, Score = 880, P = 4.2e-88 TREMBL: SPBC649_1 gene: "SPBC649.01c"; product: "putative kmesin-like protein"; S. pombe chromosome II cosmid c649., N = 3, Score = 814, P = 9.8e-86 PIR:S64238 kines n-related protein KIP3 - yeast (Saccharomyces cerevisiae), N = 2, Score = 802, P = 2.5e-83 >TREMBL:DMU89264_1 product: "kinesm like protein 67a"; Drosophila melanogaster kinesm like protein 67a mRNA, complete eds . Length = 814 HSPs : Score = 880 (132.0 bits), Expect = 4.2e-88, P = 4.2e-88 Identities = 181/345 (52%), Positives = 238/345 (68%) Query: 11 HMKVVVRVRPENTKEKAAGFHKVVHVVDKHILVFDPKQEEVSFF-HGKKTTNQNVIKKQN 69 ++KV VRVRP N +E ++ V+D+ L+FDP +E+ FF G K +++ K+ N Sbjct: 8 NIKVAVRVRPYNVRELEQKQRSIIKVMDRSALLFDPDEEDDEFFFQGAKQPYRDITKRMN 67 Query: 70 KDLKFVFDAVFDETSTQSEVFEHTTKPILRSFLNGYNCTVLAYGATGAGKTHTMLGSADE 129 K L FD VFD ++ ++FE T P++ + LNGYNC+V YGATGAGKT TMLGS Sbjct: 68 KKLTMEFDRVFDIDNSNQDLFEECTAPLVDAVLNGYNCSVFVYGATGAGKTFTMLGSEAH 127 Query: 130 PGVMYLTMLHLYKCMDEIKEEKICSTAVSYLEVYNEQIRDLLVNSGPLAVREDTQKGVVV 189 PG+ YLTM L+ + + + VSYLEVYNE + +LL SGPL +RED GVVV Sbjct: 128 PGLTYLTMQDLFDKIQAQSDVRKFDVGVSYLEVYNEHVMNLLTKSGPLKLREDNN-GVVV 186 Query: 190 HGLTLHQPKSSEEILHLLDNGNKNRTQHPTDMNATSSRSHAVFQIYLRQQDKTASINQNV 249 GL L S+EE+L +L GN +RTQHPTD NA SSRSHA+FQ+++R ++ + V Sbjct: 187 SGLCLTPIYSAEELLRMLMLGNSHRTQHPTDANAESΞRSHAIFQVHIRITERKTDTKRTV 246 Query: 250 RIAKMSLIDLAGSERASTSGAKGTRFVEGTNINRSLLALGNVINALADSKRKNQHIPYRN 309 K+S+IDLAGSERA+++ G RF EG +IN+SLLALGN IN LAD + HIPYR+ Sbjct: 247 KLSMIDLAGSERAASTKGIGVRFKEGASINKSLLALGNCINKLADGLK HIPYRD 300 Query: 310 SKLTRLLKDSLGGNCQTIMIAAVΞPSSVFYDDTYNTLKYANRAKDI 355 S LTR+LKDSLGGNC+T+M+A VS SS+ Y+DTYNTLKYA+RAK I Sbjct: 301 SNLTRILKDSLGGNCRTLMVANVSMSSLTYEDTYNTLKYASRAKKI 346 Pedant information for DKFZphtes3_26g22, frame 1 Report for DKFZphtes3_26g22.1 [LENGTH] 898 [MW] 102281.63 [pi] 9.09 [HOMOL] SWISSPROT :YB3D_SCHPO PUTATIVE KINESIN-LIKE PROTEIN C2F12.13. 3e-97 [FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YGL216w] 2e-88 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YGL216w] 2e-88 [FUNCAT] 08.22 cytoskeleton-dependent transport [S. cerevisiae, YGL216w] 2e-88 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YGL216w] 2e-88 [FUNCAT] 09.10 nuclear biogenesis [S. cerevisiae, YPR141c] 5e-42 [FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YPR141c] 5e-42 [FUNCAT] 03.13 meiosis [S. cerevisiae, YPR141c] 5e-42 [FUNCAT] 11.01 stress response [S. cerevisiae, YPR141c] 5e-42 [ FUNCAT] 03.07 pheromone response, matmg-type determination, sex-specific proteins [ [S. cerevisiae, YPR141C] 5e-42 [FUNCAT] 30.05 organization of centrosome [S. cerevisiae, YPR141c] 5e-42 [FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YKL079w] 4e-28 [BLOCKS] BL00411H [BLOCKS] BL00411G [BLOCKS] BL00411F [BLOCKS] BL00411E Kinesin motor domain proteins [BLOCKS] BL00411C Kinesin motor domain proteins [BLOCKS] BL00411B Kinesm motor domain proteins [BLOCKS] BL00411A Kinesin motor domain proteins [SCOP] d2km.l 3.29.1.5.3 Kinesm [Rat (Rattus norvegicus) le-117 [SCOP] d3kar 3.29.1.5.4 Kinesin [Baker's yeast (Ξaccharomyce le-112 [PIRKW] nucleus 6e-87 [PIRKW] heterodimer 4e-68 [PIRKW] DNA binding 9e-60 [PIRKW] heterotetramer 2e-54 [PIRKW] mitosis 9e-60 [PIRKW] microtubule binding 4e-68 [PIRKW] ATP 6e-87 [PIRKW] phosphoprotem 5e-59 [PIRKW] heterotπmer 4e-68 [PIRKW] purme nucleotide binding le-26 [PIRKW] P-loop 6e-87 [PIRKW] colled coil 4e-68 [PIRKW] heptad repeat 3e-62 [PIRKW] methylated ammo acid 2e-54 [PIRKW] hydrolase 2e-54 [PIRKW] GTP binding le-60 [PIRKW] cell division 5e-57 [SUPFAM] kmesin-related protein KIP1 3e-50 [SUPFAM] kmesin-related protein CIN8 7e-33 [SUPFAM] kinesin heavy chain 2e-54 [SUPFAM] suppressor protein SMY1 le-26 [SUPFAM] kmesin-related protein KIF3 4e-68 [SUPFAM] kinesin-related protein KIF2 le-46 [SUPFAM] kinesin-related protein unc-104 7e-60 [SUPFAM] unassigned kmesin-related proteins 6e-87 [SUPFAM] centromere protein E 3e-54 [SUPFAM] kinesin-related protein KLP61F 5e-57 [SUPFAM] kmesin-related protein MKLP-1 2e-28 [SUPFAM] pleckstrm repeat homology 7e-60 [SUPFAM] kinesin-related protein KIF1B 4e-61 [SUPFAM] kinesin motor domain homology 6e-87 [SUPFAM] kmesin-related protein KLPA le-43 [SUPFAM] kinesin-related protein nodA le-30 [SUPFAM] kinesin-related protein Eg5 5e-59 [PROSITE] ATP_GTP_A 1 [PROSITE] KINESIN_MOTOR_DOMAINl 1 [PFAM] Kinesm motor domain [KW] Irregular [KW] 3D [KW] LOW COMPLEXITY 8.57 % SEQ MSVTEEDLCHHMKVVVRVRPENTKEKAAGFHKVVHVVDKHILVFDPKQEEVSFFHGKKTT SEG 3kar- TBEEE SEQ NQNVIKKQNKDLKFVFDAVFDETSTQSEVFEHTTKPILRSFLNGYNCTVLAYGATGAGKT SEG 3kar- EEEETTTTTTEEEEEETEEETTTTCHHHHHHHHHH-HHHGGGGCCCEEEEEECTTTTCHH SEQ HTMLGSADEPGVMYLTMLHLYKCMDEIKEEKICSTAVSYLEVYNEQIRDLLVNSGPLAVR SEG 3kar- HHHHTTTT—THHHHHHHHHHHHHHHHGGGCEEEEEEEEEEEETTEEEETT-TCCCCEEE SEQ EDTQKGVVVHGLTLHQPKSSEEILHLLDNGNKNRTQHPTDMNATΞSRSHAVFQIYLRQQD SEG 3kar- EETTTEEEEETTCCEEECCGGGHHHHHHHHHHHHCCTTTTCHHHHHHCEEEEEEEEEEEE SEQ KTASINQNVRIAKMSLIDLAGSERASTSGAKGTRFVEGTNINRSLLALGNVINALADSKR SEG 3kar- TTTTCEE EEEEEEEECCCCCCCCCC HHHHHHHHHHHHHHHHHHHHHHHHTTTT SEQ KNQHIPYRNSKLTRLLKDSLGGNCQTIMIAAVSPSSVFYDDTYNTLKYANRAKDIKSSLK SEG xxxxx 3kar- TTTCCTTTTTHHHHHHGGGCTTTTEEEEEEEECCCGGGHHHHHHHHHHHHH SEQ SNVLNVNNHITQYVKICNEQKAEILLLKEKLKAYEEQKAFTNENDQAKLMISNPQEKEIE SEG xxxxxxxx xxxxxxxxxxxxxxxxxxxxx 3kar- SEQ RFQEILNCLFQNREEIRQEYLKLEMLLKENELKSFYQQQCHKQIEMMCSEDKVEKATGKR SEG xxxxxxxxxxxxx 3kar- SEQ DHRLAMLKTRRSYLEKRREEELKQFDENTNWLHRVEKEMGLLSQNGHIPKELKKDLHCHH SEG xxxxxxxxxxx 3kar- SEQ LHLQNKDLKAQIRHMMDLACLQEQQHRQTEAVLNALLPTLRKQYCTLKEAGLSNAAFESD SEG XXX 3kar- SEQ FKEIEHLVERKKVVVWADQTAEQPKQNDLPGISVLMTFPQLGPVQPIPCCSSSGGTNLVK SEG 3kar- SEQ IPTEKRTRRKLMPSPLKGQHTLKSPPSQSVQLNDSLSKELQPIVYTPEDCRKAFQNPSTV SEG 3kar- SEQ TLMKPSSFTTSFQAISSNINSDNCLKMLCEVAIPHNRRKECGQEDLDSTFTICEDIKSSK SEG 3kar- SEQ CKLPEQESLPNDNKDILQRLDPSSFSTKHSMPVPSMVPSYMAMTTAAKRKRKLTSSTSNS SEG XXXXXXXXXXXXX 3kar- SEQ SLTADVNSGFAKRVRQDNSSEKHLQENKPTMEHKRNICKINPSMVRKFGRNISKGNLR SEG XXX 3kar- Prosite for DKFZphtes3_26g22.1 PS00017 113->121 ATP_GTP_A PDOC00017 PΞ00411 252->264 KINESIN MOTOR DOMAIN1 PDOC00343 Pfam for DKFZphtes3_26g22.1 HMM_NAME Kinesin motor domain HMM *RCRPlNeREιndgcscvVQWPpWtGyktvhnghegds R+RP N +E+++G +VV + + + + +++E S Query 17 RVRPENTKEKAAGFHKVVHVVD-KHILVFDPKQEEVSFFHGKKTTNQNV 64 HMM phksFtFDHVFWWncTQedVYdtvAHPIVDDcFhGYNCTIFAYGQ + F+FD VF+ ++TQ +V++ + PI+ ++++GYNCT++AYG Query 65 IKKQNKDLKFVFDAVFDETSTQSEVFEHTTKPILRSFLNGYNCTVLAYGA 114 HMM TGSGKTYTMMGpggehPDHmGIIPRcCHDIFdrldkfqekDhdFWhVkCS TG+GKT+TM G + D+ G+ + +++++ D + + + +S Query 115 TGAGKTHTMLG SADEPGVMYLTMLHLYKCMDEIK-EEKIC-STAVS 158 HMM YMEIYNEelYDLLCPnPqhMkpLnlHEHPNMGpYVqGCTEfHVcSYeDac Y+E+YNE+I+DLL+ N ++PL+++E+ G+ V G+T+ +S E+++ Query 159 YLEVYNEQIRDLLV-N SGPLAVREDTQKGVVVHGLTLHQPKSSEEIL 204 HMM hWIWqGnknRHVAaTnMNdhSSRSHtlFTIHVeQrHk..qcdehvcHSKM H+++ GNKNR+ +T MN++SSRSH++F+I ++Q K + V++ KM Query 205 HLLDNGNKNRTQHPTDMNATSSRSHAVFQIYLRQQDKTASINQNVRIAKM 254 HMM NLVDLAGSERvnrTGAEGQRIKEGcNINqSLttLGnVInaLaDgqTKYmY +L+DLAGSER++ +GA G+R+ EG+NIN+SL++LGNVINALAD + Query 255 SLIDLAGSERASTSGAKGTRFVEGTNINRSLLALGNVINALADSK 299 HMM gghgHIPYRDSKLTWILQDSLGGNcKTcMIACIWPadWNYEETLΞTLRYA +++HIPYR SKLT+LL+DSLGGNC T MIA+++P+ + Y++T +TL+YA Query 300 RKNQHIPYRNSKLTRLLKDSLGGNCQTIMIAAVSPSSVFYDDTYNTLKYA 349 HMM dRAKnlkNkPQINEDPcamalWRrYheQIqdMKhqL* +RAK+IK + N + + + +Y + + K++ Query 350 NRAKDIKSSLKSNVLNVN-NHITQYVKICNEQKAEI 384 DKFZphtes3_27dl group: metabolism DKFZphtes3_27dl encodes a novel 712 amino acid protein similar to ubiquitm-specific proteases (EC 3.1.2.15) . The novel protein contains both, a ubiquitin carboxyl-terminal hydrolases family 2 signature 1 and signature 2. Pfam predicts a new member of the ubiquitin carboxyl-terminal hydrolases family 2. The ubiquitin system is responsible for the turn over of proteins. Ubiquitin carboxyl-terminal hydrolases (EC 3.1.2.15) (UCH) (deubiquitinatmg enzymes) are thiol proteases that recognize and hydrolyze the peptide bond at the C-terimnal glycine of ubiquitin. These enzymes are involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins. The novel protein is a new member of the ubiquitin carboxyl-terminal hydrolases family 2, represented by proteins such as yeast UBP1-16, human tre-2, human isopeptidase T and others. The novel protein can find application m modulation of ubiquitin- and protein metabolism in cells . similarity to ubiquitm-specific proteases complete cDNA, complete eds, 4 EST hits Sequenced by GBF Locus : unknown Insert length: 2871 bp Poly A stretch at pos. 2836, no polyadenylation signal found 1 CCAAACCTGA AAGAGGTTGA TTTGTAATGA TTTGCAGGGG GGCACTGGAG 51 GCAGCGGCCA GGACTTTTCA CTTAGGAGAT CAGCATTTGC CCTGATGGAA 101 ACTGGGCGAT CCTGCAGGGA CTGACCTCTG AGTTATCCAA AGGCCGACCT 151 GGGGAAAGAC TGATTTTGAG GTTTTAATAG TTTTCAGATG CTTCAAGTGT 201 TGTGAACAGA GACTTGTTTG GATTATGCAT TTCTCAGCTA GACTAAATAA 251 ATGCTAGCAA TGGATACGTG CAAACATGTT GGGCAGCTGC AGCTTGCTCA 301 AGACCATTCC AGCCTCAACC CTCAGAAATG GCACTGTGTG GACTGCAACA 351 CGACCGAGTC CATTTGGGCT TGCCTTAGCT GCTCCCATGT TGCCTGTGGA 401 AGATATATTG AAGAGCATGC ACTCAAGCAC TTTCAAGAAA GCAGTCATCC 451 TGTTGCATTG GAGGTGAATG AGATGTACGT TTTTTGTTAC CTTTGTGATG 501 ATTATGTTCT GAATGATAAC GCAACTGGAG ACCTGAAGTT ACTACGACGT 551 ACATTAAGTG CCATCAAAAG TCAAAATTAT CACTGCACAA CTCGTAGTGG 601 GAGGTTTTTA CGGTCCATGG GTACAGGTGA TGATTCTTAT TTCTTACATG 651 ACGGTGCCCA ATCTCTGCTT CAAAGTGAAG ATCAACTGTA TACTGCTCTT 701 TGGCACAGGA GAAGGATACT AATGGGTAAA ATCTTTCGAA CATGGTTTGA 751 ACAATCACCC ATTGGAAGAA AAAAGCAAGA AGAACCATTT CAGGAGAAAA 801 TAGTAGTAAA AAGAGAAGTA AAGAAAAGAC GGCAGGAATT GGAGTATCAA 851 GTTAAAGCAG AATTGGAAAG TATGCCTCCA AGAAAGAGTT TACGTTTACA 901 AGGGCTCGCT CAGTCGACCA TAATAGAAAT AGTTTCTGTT CAGGTGCCAG 951 CACAAACGCC AGCATCACCA GCAAAAGATA AAGTACTCTC TACCTCAGAA 1001 AATGAAATAT CTCAAAAAGT CAGTGACTCC TCAGTTAAAC GAAGGCCAAT 1051 AGTAACTCCT GGTGTAACAG GATTGAGAAA TTTGGGAAAT ACTTGCTATA 1101 TGAATTCTGT TCTTCAGGTG TTGAGTCATT TACTTATTTT TCGACAATGT 1151 TTTTTAAAGC TTGATCTGAA CCAATGGCTG GCTATGACTG CTAGCGAGAA 1201 GACAAGATCT TGTAAGCATC CACCAGTCAC AGATACAGTA GTATATCAAA 1251 TGAATGAATG TCAGGAAAAA GATACAGGTT TTGTTTGCTC CAGACAATCA 1301 AGTCTGTCAT CAGGACTAAG TGGTGGAGCA TCAAAAGGTA GAAAGATGGA 1351 ACTTATTCAG CCAAAGGAGC CAACTTCACA GTACATTTCT CTTTGTCATG 1401 AATTGCATAC TTTGTTCCAA GTCATGTGGT CTGGAAAGTG GGCGTTGGTC 1451 TCACCATTTG CTATGCTACA CTCAGTGTGG AGACTCATTC CTGCCTTTCG 1501 TGGTTACGCC CAACAAGACG CTCAGGAATT TCTTTGTGAA CTTTTAGATA 1551 AAATACAACG TGAATTAGAG ACAACTGGTA CCAGTTTACC AGCTCTTATC 1601 CCCACTTCTC AAAGGAAACT CATCAAACAA GTTCTGAATG TTGTAAATAA 1651 CATTTTTCAT GGACAACTTC TTAGTCAGGT TACATGTCTT GCATGTGACA 1701 ACAAATCAAA TACCATAGAA CCTTTCTGGG ACTTGTCATT GGAGTTTCCA 1751 GAAAGGTATC AATGCAGTGG AAAAGATATT GCTTCCCAGC CATGTCTGGT 1801 TACTGAAATG TTGGCCAAAT TTACAGAAAC TGAAGCTTTA GAAGGAAAAA 1851 TCTACGTATG TGACCAGTGT AACTCAAAGC GTAGAAGGTT TTCCTCCAAA 1901 CCAGTTGTAC TCACAGAAGC CCAGAAACAA CTTATGATAT GCCACCTACC 1951 TCAGGTTCTC AGACTGCACC TCAAACGATT CAGGTGGTCA GGACGTAATA 2001 ACCGAGAGAA GATTGGTGTT CATGTTGGCT TTGAGGAAAT CTTAAACATG 2051 GAGCCCTATT GCTGCAGGGA GACCCTGAAA TCCCTCAGAC CAGAATGCTT 2101 TATCTATGAC TTGTCCGCGG TGGTGATGCA CCATGGGAAA GGATTTGGCT 2151 CAGGGCACTA CACTGCCTAC TGCTATAATT CTGAAGGAGG GTTCTGGGTA 2201 CACTGCAATG ATTCCAAACT AAGCATGTGC ACTATGGATG AAGTATGCAA 2251 GGCTCAAGCT TATATCTTGT TTTATACCCA ACGAGTTACT GAGAATGGAC 2301 ATTCTAAACT TTTGCCTCCA GAGCTCCTGT TGGGGAGCCA ACATCCCAAT 2351 GAAGACGCTG ATACCTCGTC TAATGAAATC CTTAGCTGAT CCAAAGACAA 2401 TGGGGTTTTC TTCCTGTGAT TTATATATAT ACTTTTTAAA AGACTGATGT 2451 ACCATTTTAA ACTTCATTTT TTCTTGTGAA TCAGTGTATA CTACATTTAT 2501 ACATTTTATA TCTAACAATT TTTTTTTTTT ACAAAGTATA AATGTATATA 2551 TCAACTGAAG GTAACTACTT TTTTCATATT TGGAGTTTTA AACTTTTGGT 2601 GTTTACCTCA GACTGATGTT ACCTCTTTTA TATTTTTATG TCTTAATTGG 2651 CTCGGATGAT GAACTTGTGC AATCTTCTAC CAACAAAGTT CAAGTGGCAT 2701 CATTTTATAT ACATGTATCT TTTTCAGGTA TTTTCTATAC AAATTCTTAA 2751 TAGATGGAAA ATTAGACTCT AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 2801 AAAAAAAAAA AAAAAAAAAA AAGGGGCGGC CGCTCTAAAA AAAAAAAAAA 2851 AAAAAAAAAA AAAAAAAAAG G BLAST Results No BLAST result Medline entries 98072201: Regulation of ubiquitin-dependent processes by deubiquitmating enzymes . 98431658: The ubiquitin system. Peptide information for frame 2 ORF from 251 bp to 2386 bp; peptide length: 712 Category: similarity to known protein Prosite motifs: UCH_2_1 (274-290) UCH_2_2 (619-638) UCH 2 2 (619-638) 1 MLAMDTCKHV GQLQLAQDHS SLNPQKWHCV DCNTTESIWA CLSCSHVACG 51 RYIEEHALKH FQESSHPVAL EVNEMYVFCY LCDDYVLNDN ATGDLKLLRR 101 TLSAIKSQNY HCTTRSGRFL RSMGTGDDSY FLHDGAQSLL QSEDQLYTAL 151 WHRRRILMGK IFRTWFEQSP IGRKKQEEPF QEKIVVKREV KKRRQELEYQ 201 VKAELESMPP RKSLRLQGLA QSTIIEIVSV QVPAQTPASP AKDKVLSTSE 251 NEISQKVSDS SVKRRPIVTP GVTGLRNLGN TCYMNSVLQV LΞHLLIFRQC 301 FLKLDLNQWL AMTASEKTRS CKHPPVTDTV VYQMNECQEK DTGFVCSRQS 351 SLSSGLSGGA SKGRKMELIQ PKEPTSQYIS LCHELHTLFQ VMWSGKWALV 401 SPFAMLHSVW RLIPAFRGYA QQDAQEFLCE LLDKIQRELE TTGTSLPALI 451 PTSQRKLIKQ VLNVVNNIFH GQLLSQVTCL ACDNKSNTIE PFWDLSLEFP 501 ERYQCSGKDI ASQPCLVTEM LAKFTETEAL EGKIYVCDQC NSKRRRFSSK 551 PVVLTEAQKQ LMICHLPQVL RLHLKRFRWS GRNNREKIGV HVGFEEILNM 601 EPYCCRETLK SLRPECFIYD LSAVVMHHGK GFGSGHYTAY CYNSEGGFWV 651 HCNDSKLSMC TMDEVCKAQA YILFYTQRVT ENGHSKLLPP ELLLGSQHPN 701 EDADTSSNEI LS BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_27dl, frame 2 PIR:S57591 hypothetical protein YMR223w - yeast (Saccharomyces cerevisiae), N = 4, Score = 218, P = 8.4e-38 SWISSPROT :UBPB_HUMAN UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 11 (EC 3.1.2.15) (UBIQUITIN THIOLESTERASE 11) (UBIQUITIN-SPECIFIC PROCESSING PROTEASE 13) (DEUBIQUITINATING ENZYME 11) (KIAA0055)., N = 2, Score = 300, P = 9.3e-31 TREMBL:AF079565_1 gene: "Ubp41"; product: "ubiquitm-specific protease UBP41"; Mus musculus ubiquitm-specific protease UBP41 (Ubp41) mRNA, complete eds., N = 3, Score = 187, P = 8.7e-30 PIR: 158376 hypothetical protein unp - mouse, N = 3, Score = 214, P = 1.2e-28 >SWISSPROT:UBPB_HUMAN UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 11 (EC 3.1.2.15) (UBIQUITIN THIOLESTERASE 11) (UBIQUITIN-SPECIFIC PROCESSING PROTEASE 13) (DEUBIQUITINATING ENZYME 11) (KIAA0055) . Length = 1,118 HSPs: Score = 300 (45.0 bits), Expect = 9.3e-31, Sum P(2) = 9.3e-31 Identities = 95/301 (31%), Positives = 149/301 (49%) Query: 381 LCHELHTLFQVMWΞGKWALVSPFAMLHSVWRLIPAFRGYAQQDAQEFLCELLDKIQREL- 439 + E + + +W+G++ +SP ++ ++ F GY+QQD+QE L L+D + +L Sbjct: 826 VAEEFGIIMKALWTGQYRYISPKDFKITIGKINDQFAGYSQQDSQELLLFLMDGLHEDLN 885 Query: 440 ETTGTSLPALIPTSQRKLIKQVLN—VVNNIFHGQLLSQVTCLACDNKSNT 488 E L + LN ++ +F GQ S V CL C KS T Sbjct: 886 KADNRKRYKEENNDHLDDFKAAEHAWQKHKQLNESIIVALFQGQFKΞTVQCLTCHKKSRT 945 Query: 489 IEPFWDLSLEFPERYQCSGKDIASQPCLVTEMLAKFTETEALEGKIYVCDQCNSKRRRFS 548 E F LSL +C+ +D CL + +K E + + + C C ++R Sbjct: 946 FEAFMYLSLPLASTSKCTLQD CL--RLFSK—EEKLTDNNRFYCSHCRARR 992 Query: 549 SKPVVLTEAQKQLMICHLPQVLRLHLKRFRWSGRNNREKIGVHVGFE-EILNMEPYCC— 605 ++ K++ I LP VL +HLKRF + GR ++K+ V F E L++ Y Sbjct: 993 DSLKKIEIWKLPPVLLVHLKRFSYDGRW-KQKLQTSVDFPLENLDLSQYVIGP 1044 Query: 606 RETLKSLRPECFIYDLSAVVMHHGKGFGSGHYTAYCYNSEGGFWVHCNDSKLSMCTMDEV 665 + LK Y+L +V H+G G GHYTAYC N+ W +D ++S ++ V Sbjct: 1045 KNNLKK YNLFSVSNHYG-GLDGGHYTAYCKNAARQRWFKFDDHEVSDISVSSV 1096 Query: 666 CKAQAYILFYTQ RVTE 681 + AYILFYT RVT+ Sbjct: 1097 KSΞAAYILFYTSLGPRVTD 1115 Score = 126 (18.9 bits), Expect = 9.3e-31, Sum P(2) = 9.3e-31 Identities = 41/116 (35%), Positives = 63/116 (54%) Query: 200 QVKAELESMPPR—KSLRLQGLAQSTIIEIVSVQVPAQTPASPAKDKVLSTSENEISQKV 257 Q+ AE + P + +S + Q+ 1+ + P TP ++K + EIS ++ Sbjct: 701 QIPAERDREPSKLKRSYSSPDITQA—IQEEEKRKPTVTPTVNRENKPTCYPKAEIS-RL 757 Query: 258 SDΞSVKR-RPIVT PGVTGLRNLGNTCYMNSVLQVLS HLLIF--RQCFLKLDLNQ 308 S S ++ P+ P +TGLRNLGNTCYMNS+LQ L HL + R C+ D+N+ Sbjct: 758 ΞASQIRNLNPVFGGSGPALTGLRNLGNTCYMNSILQCLCNAPHLADYFNRNCYQD-DINR 816 Score = 50 (7.5 bits), Expect = 8.3e-23, Sum P(2) = 8.3e-23 Identities = 29/106 (27%), Positives = 51/106 (48%) Query: 173 RKKQEEPFQEKIVVKREVKKRRQELEYQVKAELESMPPRKSLRLQGLAQSTIIEIVSVQV 232 + KQE+ +E+ +++ K R++E E + K + E+ + Q A+ + + S Q Sbjct: 475 KNKQEKELRERQQEEQKEKLRKEEQEQKAKKKQEA-EENEITEKQQKAKEEMEKKESEQA 533 Query: 233 PAQ TPASPAKD KVLSTSENEIS—QKVΞDSSVKRRPIVTPGV 272 + T A K+ K S SE+E S +K + KR P TP + Sbjct: 534 KKEDKETSAKRGKEITGVKRQSKSEHETSDAKKSVEDRGKRCP—TPEI 580 Score = 42 (6.3 bits), Expect = 5.7e-22, Sum P(2) = 5.7e-22 Identities = 13/58 (22%), Positives = 27/58 (46%) Query: 167 EQSPIGRKKQEEPFQEKIVVKREVKKRRQELEY-QVKAELESMPPRKSLRLQGLAQΞT 223 EQ +KKQE E +++ K+ ++ E Q K E + ++ + G+ + + Sbjct: 498 EQEQKAKKKQEAEENEITEKQQKAKEEMEKKESEQAKKEDKETΞAKRGKEITGVKRQS 555 Pedant information for DKFZphtes3_27dl, frame 2 Report for DKFZphtes3_27dl .2 [LENGTH] 712 [MW] 81155.71 [pi] 8.21 [HOMOL] SWISSPROT :UBPB_HUMAN UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 11 (EC 3.1.2.15) (UBIQUITIN THIOLESTERASE 11) (UBIQUITIN-SPECIFIC PROCESSING PROTEASE 13) (DEUBIQUITINATING ENZYME 11) (KIAA0055) . 4e-32 [FUNCAT] 06.13.01 cytoplasmic degradation [S. cerevisiae, YMR223w] 5e-33 [FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylation, farnesylation and processing) [S. cerevisiae, YMR223w] 5e-33 [ FUNCAT ] 06.13 proteolysis [S. cerevisiae, YBL067c] 3e-19 [ FUNCAT ] 10.03.99 other osmosensing activities [S. cerevisiae, YDR069c] 2e-17 [FUNCAT] 03.10 sporulation and germination [S. cerevisiae, YDR069c] 2e-17 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YDR069c] 2e-17 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YDR069c] 2e-17 [FUNCAT] 09.25 vacuolar and lysosomal biogenesis [S. cerevisiae, YDR069c] 2e-17 [FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YNL186w] 4e-17 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YHLOlOc] 3e-12 [BLOCKS] BL00970A Nuclear transition protein 2 proteins [BLOCKS] BL00972D [BLOCKS] BL00972C [BLOCKS] BL00972B [BLOCKS] BL00972A [EC] 3.1.2.15 Ubiquitin thiolesterase 5e-06 [PIRKW] alternative splicing 2e-ll [PIRKW] thiolester hydrolase 5e-06 [PIRKW] hydrolase le-14 [SUPFAM] RING finger homology 7e-ll [SUPFAM] deubiqumatmg enzyme SSV7 5e-16 [PROSITE] MYRISTYL 5 [PROSITE] AMIDATION 2 [PROSITE] CAMP_PHOSPHO_SITE 1 [PROSITE] CK2_PHOSPHO_SITE 10 [PROSITE] TYR_PHOSPHO_SITE 2 [PROSITE] UCH_2_21 [PROSITE] PKC_PHOSPHO_SITE 17 [PROSITE] ASN_GLYCOSYLATION 4 [PROSITE] UCH_2_11 [PFAM] Ubiquitin carboxyl-terminal hydrolases family 2 [PFAM] Ubiquitin carboxyl-terminal hydrolases family 2 [KW] Alpha_Beta [KW] LOW COMPLEXITY 4.92 % SEQ MLAMDTCKHVGQLQLAQDHSSLNPQKWHCVDCNTTESIWACLSCSHVACGRYIEEHALKH SEG PRD ccccccccchhhhhhhhcccccccccceeecccceeeeeeeccccccccchhhhhhhhhh SEQ FQESSHPVALEVNEMYVFCYLCDDYVLNDNATGDLKLLRRTLSAIKSQNYHCTTRSGRFL SEG PRD hhhhccceeecccceeeeeeccccccccccccchhhhhhhhhhhhhcccceeeccccccc SEQ RSMGTGDDSYFLHDGAQSLLQSEDQLYTALWHRRRILMGKIFRTWFEQSPIGRKKQEEPF SEG PRD cccccccccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhchhhhhhhhhhh SEQ QEKIVVKREVKKRRQELEYQVKAELESMPPRKΞLRLQGLAQSTIIEIVSVQVPAQTPASP SEG xxxxxxxxxxxxxxxx PRD hheeehhhhhhhhhhhhhhhhhhhhhhcccccccccccccccceeeeeeccccccccccc SEQ AKDKVLSTΞENEISQKVSDSSVKRRPIVTPGVTGLRNLGNTCYMNSVLQVLSHLLIFRQC SEG PRD ccccccccccccccccccccccccccccccccccccccccccchhhhhhhhhhhhhhhhh SEQ FLKLDLNQWLAMTASEKTRSCKHPPVTDTVVYQMNECQEKDTGFVCSRQSSLSSGLSGGA SEG xxxxxxxxxxxxxx PRD hhhhhhchhhhhhhhhhhhhhccccccceeehhhhhcccccccccccccccccccccccc SEQ SKGRKMELIQPKEPTSQYISLCHELHTLFQVMWSGKWALVSPFAMLHSVWRLIPAFRGYA SEG xxxxx PRD ccccceeecccccccchhhhhhhhhhhhhhhhhccceeeeccchhhhhhhhhhhccccch SEQ QQDAQEFLCELLDKIQRELETTGTSLPALIPTSQRKLIKQVLNVVNNIFHGQLLSQVTCL SEG PRD hhhhhhhhhhhhhhhhhhhhhhccccccccccchhhhhhhhhhhhhccccchhhhhhhhc SEQ ACDNKSNTIEPFWDLSLEFPERYQCSGKDIASQPCLVTEMLAKFTETEALEGKIYVCDQC SEG PRD cccccccccccccccccccccccccccccccccchhhhhhhhhhhhhhhhccceeecccc SEQ NSKRRRFSSKPVVLTEAQKQLMICHLPQVLRLHLKRFRWSGRNNREKIGVHVGFEEILNM SEG PRD ccccccccccchhhhhhhhhhhhhhchhhhhhhhhhhhhcccccccccceeeeccccccc SEQ EPYCCRETLKSLRPECFIYDLSAVVMHHGKGFGSGHYTAYCYNSEGGFWVHCNDSKLSMC SEG PRD ccccccccccccccceeeeeeeeeeeecccccccccceeeeccccccceeeecccccccc SEQ TMDEVCKAQAYILFYTQRVTENGHSKLLPPELLLGSQHPNEDADTSSNEILS SEG PRD cchhhhhhhhhhhhhheeeecccccccccccccccccccccccccccccccc DKFZphtes3_27k4 group: transmembrane protein Summary DKFZphtes3_27k4 encodes a novel 490 ammo acid protein with similarity to two hypothetical C. elegans proteins. The novel protein contains 10 transmembrane regions and a leucine zipper. It is a member of the new 10 trans-membrane domain containing protein family which is specific for multicellular eukariotes . No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes and as a new marker for testicular cells. strong similarity to C. elegans K07H8.2/ZK185.2 membrane regions: 10 complete cDNA, complete eds potential start at Bp 109, few EST hits Sequenced by GBF Locus : unknown Insert length: 1901 bp Poly A stretch at pos. 1866, no polyadenylation signal found 1 GTGATTTACC AGAAAAACCA AGAAGACAGG CACAAAAAAG CAAACGGCAT 51 TTGGCAAGAT GGATTATCAA CTGCAGTACA GACTTTTAGT AATAGATCTG 101 AGCAACACAT GGAGTATCAC AGTTTCTCAG AGCAGTCTTT TCATGCCAAT 151 AATGGGCACG CATCATCAAG CTGCAGCCAA AAGTATGATG ACTATGCCAA 201 TTATAATTAC TGTGATGGAA GGGAGACTTC AGAAACCACT GCCATGTTAC 251 AAGATGAAGA TATATCTAGT GATGGTGATG AAGATGCTAT TGTAGAAGTG 301 ACCCCAAAAT TACCAAAGGA ATCCAGTGGC ATCATGGCAT TGCAAATACT 351 TGTGCCCTTT TTGCTAGCTG GTTTTGGAAC AGTTTCAGCT GGCATGGTAC 401 TGGATATAGT ACAGCACTGG GAGGTGTTCA GAAAAGTTAC AGAAGTTTTC 451 ATTTTAGTCC CTGCACTTCT TGGTCTCAAA GGGAACTTGG AAATGACATT 501 GGCATCCAGA TTATCCACTG CAGTAAATAT TGGGAAGATG GATTCACCCA 551 TTGAAAAGTG GAACCTAATA ATTGGCAACT TGGCTTTAAA GCAGGTTCAG 601 GCAACAGTAG TGGGTTTTCT AGCAGCTGTG GCAGCAATTA TATTGGGCTG 651 GATTCCAGAA GGAAAATATT ACCTTGATCA TTCCATACTT CTGTGCTCTA 701 GCAGTGTGGC AACTGCCTTC ATTGCATCTC TTCTGCAGGG AATAATAATG 751 GTTGGGGTTA TCGTTGGTTC AAAGAAGACT GGTATAAATC CTGATAATGT 801 TGCTACACCC ATTGCTGCTA GTTTTGGCGA CCTTATAACT CTTGCCATAT 851 TGGCTTGGAT AAGTCAGGGC TTATACTCCT GTCTTGAGAC CTATTACTAC 901 ATTTCTCCAT TAGTTGGTGT ATTTTTCTTG GCTCTAACCC CTATTTGGAT 951 TATAATAGCT GCCAAACATC CAGCCACAAG AACAGTTCTC CACTCAGGCT 1001 GGGAGCCTGT CATAACAGCT ATGGTTATAA GTAGCATTGG GGGCCTTATT 1051 CTGGACACAA CTGTATCAGA CCCAAACTTG GTTGGGATTG TTGTTTACAC 1101 GCCAGTTATT AATGGTATTG GTGGTAATTT GGTGGCCATT CAGGCTAGCA 1151 GGATTTCTAC CTACCTCCAT TTACATAGCA TTCCAGGAGA ATTGCCTGAT 1201 GAACCCAAAG GTTGTTACTA CCCATTTAGA ACTTTCTTTG GTCCAGGAGT 1251 AAATAATAAG TCTGCTCAAG TTCTACTGCT TTTAGTGATT CCTGGACATT 1301 TAATTTTCCT CTACACTATT CATTTGATGA AAAGTGGTCA TACTTCTTTA 1351 ACTATAATCT TCATAGTAGT GTATTTATTT GGCGCTGTGT TACAGGTATT 1401 TACCTTGCTG TGGATTGCTG ACTGGATGGT CCATCACTTC TGGAGGAAAG 1451 GAAAGGACCC GGATAGTTTC TCCATCCCCT ACCTAACAGC ATTGGGTGAT 1501 CTGCTCGGGA CAGCTCTGTT AGCCTTAAGT TTTCATTTTC TTTGGCTTAT 1551 TGGAGATCGA GATGGAGATG TTGGAGACTA ATAAATTCTA CAAACTGCTC 1601 TCAAGTTACC AAGGAAGAAA ATACACGACA ACCACTTATG GCTCTTTTTC 1651 AAAACTCTTA AATCAGTAGT TTGACTTTTG CCAGGGTAAT CTTCAGTTGG 1701 CCCTGATTCA ATTAAATGGC CTTAATTTTT TTTTAAGGAA TTTGTGTCAA 1751 AACCAGAATG AAGAGTATTC GTGCTGCTTT TCATAGAATA AATGATAATT 1801 TGACATAGAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 1851 AAAAAAAAAA AAGGGGAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAGGG 1901 G BLAST Results o BLAST result Medline entries No Medlme entry Peptide information for frame 1 ORF from 109 bp to 1578 bp; peptide length: 490 Category: similarity to unknown protein 1 MEYHΞFSEQS FHANNGHASS SCSQKYDDYA NYNYCDGRET SETTAMLQDE 51 DISSDGDEDA IVEVTPKLPK ESSGIMALQI LVPFLLAGFG TVSAGMVLDI 101 VQHWEVFRKV TEVFILVPAL LGLKGNLEMT LASRLSTAVN IGKMDSPIEK 151 WNLIIGNLAL KQVQATVVGF LAAVAAIILG WIPEGKYYLD HSILLCSSSV 201 ATAFIASLLQ GIIMVGVIVG SKKTGINPDN VATPIAASFG DLITLAILAW 251 ISQGLYSCLE TYYYISPLVG VFFLALTPIW IIIAAKHPAT RTVLHSGWEP 301 VITAMVISSI GGLILDTTVS DPNLVGIVVY TPVINGIGGN LVAIQASRIS 351 TYLHLHSIPG ELPDEPKGCY YPFRTFFGPG VNNKSAQVLL LLVIPGHLIF 401 LYTIHLMKSG HTSLTIIFIV VYLFGAVLQV FTLLWIADWM VHHFWRKGKD 451 PDSFSIPYLT ALGDLLGTAL LALSFHFLWL IGDRDGDVGD BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_27k4, frame 1 TREMBL :AF036704_2 gene: "ZK185.2"; Caenorhabditis elegans cosmid ZK185., N = 1, Score = 730, P = 3.1e-72 TREMBL:AF047659_9 gene: "K07H8.2"; Caenorhabditis elegans cosmid K07H8., N = 1, Score = 940, P = 1.7e-94 >TREMBL:AF047659_9 gene: "K07H8.2"; Caenorhabditis elegans cosmid K07H8. Length = 507 HSPs: Score = 940 (141.0 bits), Expect = 1.7e-94, P = 1.7e-94 Identities = 204/412 (49%), Positives = 271/412 (65%) Query: 68 LPKESSGIMALQILVPFLLAGFGTVSAGMVLDIVQHWEVFRKVTEVFILVPALLGLKGNL 127 +P ESS ++ Q+L PF +AG G V AG+VL IV W +F ++ E+ ILVPALLGLKGNL Sbjct: 82 IPAESSYVLFFQVLFPFAVAGLGMVFAGLVLSIVVTWPLFEEIPEILILVPALLGLKGNL 141 Query: 128 EMTLASRLSTAVNIGKMDSPIEKWNLIIGNLALKQVQATVVGFLAAVAAIILGWIPEGKY 187 EMTLASRLST N+G MDS ++ +++I NLAL QVQATVV FLA+ A L +IP G + Sbjct: 142 EMTLASRLSTLANLGHMDSSKQRKDVVIANLALVQVQATVVAFLASAFAAALAFIPSGDF 201 Query: 188 YLDHSILLCSSSVATAFIASLLQGIIMVGVIVGSKKTGINPDNVATPIAASFGDLITLAI 247 H L+C+SS+ATA ASL+ ++MV VIV S+K INPDNVATPIAAΞ GDL TL + Sbjct: 202 DWAHGALMCASSLATACSASLVLSLLMVVVIVTSRKYNINPDNVATPIAASLGDLTTLTV 261 Query: 248 LAWISQGLYSCLETYYYISPLVGVFFLALTPIWIIIAAKHPATRTVLHSGWEPVITAMVI 307 LA+ T +++ +V V FL L P WI IA ++ T+ L++GW PVI +M+I Sbjct: 262 LAFFGSVFLKAHNTEΞWLNVIVIVLFLLLLPFWIKIANENEGTQETLYNGWTPVIMSMLI 321 Query: 308 SSIGGLILDTTVSDPNLVGIVVYTPVINGIGGNLVAIQASRISTYLHLHSIPGELPDEPK 367 SS GG IL+T V + + Y PV+NG+GGNL A+QASR+STY H G LP+E Sbjct: 322 SSAGGFILETAVRRYH--SLSTYGPVLNGVGGNLAAVQASRLSTYFHKAGTVGVLPNEWT 379 Query: 368 GCYYPF—RTFFGPGVNNKSAQVLLLLVIPGHLIFLYTIHLM KSGHTSLTIIFIVV 421 + R FF +++SA+VLLLLV+PGH+ F + I L K+ T +F + Sbjct: 380 VSRFTSVQRAFFSKEWDSRSARVLLLLVVPGHICFNFLIQLFTLTSKNNVTPHGPLFTSL 439 Query: 422 YLFGAVLQVFTLLWIADWMVHHFWRKGKDPDSFSIPYLTALGDLLGTALLALSF 475 Y+ A++QV LL++ +V W+ DPD+ IPYLTALGDLLGT LL + F Sbjct: 440 YMIAAIIQVVILLFVCQLLVALLWKWKIDPDNSVIPYLTALGDLLGTGLLFIVF 493 Pedant information for DKFZphtes3_27k4, frame 1 Report for DKFZphtes3_27k4.1 [LENGTH] 490 [MW] 53266.39 [pi] 5.29 [HOMOL] TREMBL :AF047659_9 gene: "K07H8.2"; Caenorhabditis elegans cosmid K07H8. 4e-94 [PROSITE] LEUCINE_ZIPPER 1 [PROSITE] MYRISTYL 7 [PROSITE] CAMP_PHOSPHO_SITE 1 [PROSITE] CK2_PHOSPHO_SITE 7 [PROSITE] PROKAR_LIPOPROTEIN 2 [PROSITE] TYR_PHOSPHO_SITE 1 [PROSITE] PKC_PHOSPHO_SITE 3 [PROSITE] ASN_GLYCOSYLATION 1 [KW] TRANSMEMBRANE 10 [KW] LOW COMPLEXITY 3.06 SEQ MEYHSFSEQSFHANNGHASSSCSQKYDDYANYNYCDGRETSETTAMLQDEDISSDGDEDA SEG PRD cccccccceeeccccccccccccccccccceeecccccccchhhhhhhhcccccccccee MEM SEQ IVEVTPKLPKESSGIMALQILVPFLLAGFGTVΞAGMVLDIVQHWEVFRKVTEVFILVPAL SEG PRD eeeeeccccccchhhhhhhhhhhhhhhcccchhhhhhhhhcchhhhhcccceeeeeeccc MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMM . MMMMMMMMMMMMMMM SEQ LGLKGNLEMTLASRLSTAVNIGKMDSPIEKWNLIIGNLALKQVQATVVGFLAAVAAIILG SEG PRD ccccchhhhhhhhhhhhhhccccccccccceeeehhhhhhhhhhhhhhhhhhhhhhhhhh MEM MMMMMMM MMMMMMMMMMMMMMMMM SEQ WIPEGKYYLDHSILLCSSSVATAFIASLLQGIIMVGVIVGSKKTGINPDNVATPIAASFG SEG PRD hcccceeecccceeehhhhhhhhhhhhhhhhhhhhheeeecccccccccccccccccccc MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM MMMMMM SEQ DLITLAILAWISQGLYSCLETYYYISPLVGVFFLALTPIWIIIAAKHPATRTVLHSGWEP SEG PRD cchhhhhhhhhhhhhhhhcceeeeehhhhhhhhhhchhhhhhhhccccccccchhhhhhh MEM MMMMMMMMMMMMMMM....MMMMMMMMMMMMMMMMMMMMM MMMMMM SEQ VITAMVISSIGGLILDTTVSDPNLVGIVVYTPVINGIGGNLVAIQASRISTYLHLHSIPG SEG PRD hcchhhhhhcceeeeccccccccceeeeeeceeeecccccceeeeehhhhhhhhhhcccc MEM MMMMMMMMMMMMMMMM SEQ ELPDEPKGCYYPFRTFFGPGVNNKSAQVLLLLVIPGHLIFLYTIHLMKΞGHTSLTI IFIV SEG PRD cccccccccccceeeeeccccchhhhhhhhhhccccchhhhhhhhcccccccceeeehhh MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMM...MMMMMMM SEQ VYLFGAVLQVFTLLWIADWMVHHFWRKGKDPDSFSIPYLTALGDLLGTALLALSFHFLWL SEG xxxxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhcccccccceeeeeecchhhhhhhhhhhhheeee MEM MMMMMMMMMMMMMMMMMMMMMM MMMMMMMMMMMMMMMMMMMMMMMM SEQ IGDRDGDVGD SEG PRD eecccccccc MEM MM Prosite for DKFZphtes3_27k4.1 PS00001 383->387 ASN_GLYCOSYLATION PDOC00001 PΞ00004 108->112 CAMP_PHOSPHO_SITE PDOC00004 PS00005 23->26 PKC_PHOSPHO_SITE PDOC00005 PS00005 65->68 PKC_PHOSPHO_SITE PDOC00005 PS00005 221->224 PKC_PHOSPHO_SITE PDOC00005 PS00006 5->9 CK2_PHOSPHO_SITE PDOC00006 PS00006 54->58 CK2_PHOSPHO_SITE PDOC00006 PS00006 146->150 CK2_PHOSPHO_SITE PDOC00006 PS00006 238->242 CK2_PHOSPHO_ΞITE PDOC00006 PS00006 257->261 CK2_PHOSPHO_SITE PDOC00006 PS00006 296->300 CK2_PHOSPHO_SITE PDOC00006 PS00006 318->322 CK2_PHOΞPHO_SITE PDOC00006 PS00007 25->33 TYR_PHOSPHO_SITE PDOC00007 PS00008 90->96 MYRISTYL PDOC00008 PS00008 122-M28 MYRISTYL PDOC00008 PS00008 216->222 MYRISTYL PDOC00008 PS00008 220->226 MYRISTYL PDOC00008 PS00008 254->260 MYRISTYL PDOC00008 PS00008 336->342 MYRISTYL PDOC00008 PS00008 339->345 MYRISTYL PDOC00008 PS00013 12->23 PROKAR_LIPOPROTEIN PDOC00013 PS00013 248->259 PROKAR_LIPOPROTEIN PDOC00013 PS00029 459->481 LEUCINE ZIPPER PDOC00029 (No Pfam data available for DKFZphtes3_27k4.1) DKFZphtes3_27ol4 group: testes derived DKFZphtes3_27ol4 encodes a novel 358 am o acid protein with similarity to C. elegans cosmid C55A6. The new protein contains a C3HC4 zmc finger (RING finger) signature. The ring fmger structure binds two atoms of zinc, and is involved in mediating protem-protem interactions. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application m studying the expression profile of testis-specific genes . similarity to C. elegans C55A6.1 complete cDNA, complete eds, EST hits Sequenced by GBF Locus: /map="6" Insert length: 2158 bp Poly A stretch at pos. 2137, polyadenylation signal at pos. 2120 1 CCGAGGCCAG AGAGAAAAGA CTGCGAGGTG GCCGCAGCTG TGGCCGGAGA 51 GCACAAAGAA TGAACCAGCA GTGGAAGAGA AAATACTGTA AGCTGGCTGA 101 CTGCTGGTGA AGAAAATGCT TTATTTTTGT GGCAGGCATC TGTGGGATCT 151 GTAATAGAAA TATATTGGAG TAATTCAAGA TTCTGTGGTT GGCCCTTTTG 201 ACTGCTCTCT CTACAGGTTT AATTTGGGCA TTTACTCATT TTCATGGCTC 251 CAAGGACCAT GTATGTGTTG GGGATCTTCA ATATTCATGT TATTTTCTCC 301 TTTGGTCTTA TATGATTGTT ACCTTTATGA AGCTTTAGTG ATTACAAAGC 351 ACTTTTTTTG TCCATTTTTA CCTGAGCTTT GTAAACTCTG ATTTGCAGGA 401 TGGCTGGCTG TGGTGAAATT GATCATTCAA TAAACATGCT TCCTACAAAC 451 AGGAAAGCGA ACGAGTCCTG TTCTAATACT GCACCTTCTT TAACCGTCCC 501 TGAATGTGCC ATTTGTCTGC AAACATGTGT TCATCCAGTC AGTCTGCCCT 551 GTAAGCACGT TTTCTGCTAT CTATGTGTAA AAGGAGCTTC ATGGCTTGGA 601 AAGCGGTGTG CTCTTTGTCG ACAAGAAATT CCCGAGGATT TCCTTGACAA 651 GCCAACCTTG TTGTCACCAG AAGAACTCAA GGCAGCAAGT AGAGGAAATG 701 GTGAATATGC ATGGTATTAT GAAGGAAGAA ATGGGTGGTG GCAGTACGAT 751 GAGCGCACTA GTAGAGAGCT GGAAGATGCT TTTTCCAAAG GTAAAAAGAA 801 CACTGAAATG TTAATTGCTG GCTTTCTGTA TGTCGCTGAT CTTGAAAACA 851 TGGTTCAATA TAGGAGAAAT GAACATGGAC GTCGCAGGAA GATTAAGCGA 901 GATATAATAG ATATACCAAA GAAGGGAGTA GCTGGACTTA GGCTAGACTG 951 TGATGCTAAT ACCGTAAACC TAGCAAGAGA GAGCTCTGCT GACGGAGCGG 1001 ACAGTGTATC AGCACAGAGT GGAGCTTCTG TTCAGCCCCT AGTGTCTTCT 1051 GTAAGGCCCC TAACATCAGT AGATGGTCAG TTAACAAGCC CTGCAACACC 1101 ATCCCCTGAT GCAAGCACTT CTCTGGAAGA CTCTTTTGCT CATTTACAAC 1151 TCAGTGGAGA CAACACAGCT GAAAGGAGTC ATAGGGGAGA AGGAGAAGAA 1201 GATCATGAAT CACCATCTTC AGGCAGGGTA CCAGCACCAG ACACCTCCAT 1251 TGAAGAAACT GAATCAGATG CCAGTAGTGA TAGTGAGGAT GTATCTGCAG 1301 TTGTTGCACA GCACTCCTTG ACCCAACAGA GACTTTTGGT TTCTAATGCA 1351 AACCAGACAG TACCCGATCG ATCAGATCGA TCGGGAACTG ATCGATCAGT 1401 AGCAGGGGGT GGAACAGTGA GTGTCAGTGT CAGATCTAGA AGGCCTGATG 1451 GACAGTGCAC AGTAACTGAA GTTTAAATAA AAATGTCTTC AGCTCCATGC 1501 TCAAGGTTGA AAGGGTTACC TGTAAATTTC TGCCCACATA ACATTATACT 1551 CATCCCTAGT AGTGCATTTT GGGAGTTGGG GTGGGAAGGG GTATGGGAAG 1601 GATAGACTCA TAATTAAAAT GTCTAACATG TCTCTGTTGA GAAATTTATT 1651 TAATGTAAGG AACTTGGGTG TTAATAGTTG AGAGCTGTTT AGTAATAACC 1701 CAGTTTTCTT GAGGTCTGTT TACTTTATAC TTTTTAAAAA CTTCTGTAGT 1751 TCTTTTGGCC AGTGTGTTTG TATTATCTGT GCATTAATGG TCCTCATCTG 1801 ACTCCTGCAT TGTGTCTTAT TTTTCTGCAT GGATTGGCAT AAGACCATTA 1851 CTAAAATTTG GCACCTGTGA GATGTTTGAT ATTATGAACA GGAAACATAA 1901 TTTAATGTAT GAATAGATGT GAATTTGGGA TTTCAAAATA GATGAATAAC 1951 AACTATTTTA TAGTAAAGTT ATTGAAATGG AAATGAAAAC AGCCAGTAAC 2001 TTATGTTTCA GAATGTTTGT AACACACTTC ATGGTGTTCC CATAGGCTTT 2051 GCTGTCTAGT CTTATAGTTT GAGGTTTTTT TGGTCTGCAT TTTTCTTTTT 2101 GATTACAAAA TTTATAATTT AATAAATACT AGAGTTTATC AAAAAAAAAA 2151 AAAAAAAG BLAST Results Entry HSG117 from database EMBL: human STS SHGC-36270. Score = 1148, P = 8.9e-45, identities = 240/250 Medl e entries No Medline entry Peptide information for frame 1 ORF from 400 bp to 1473 bp; peptide length: 358 Category: similarity to unknown protein Prosite motifs: ZINC FINGER C3HC4 (51-61) 1 MAGCGEIDHS INMLPTNRKA NESCSNTAPS LTVPECAICL QTCVHPVSLP 51 CKHVFCYLCV KGASWLGKRC ALCRQEIPED FLDKPTLLSP EELKAASRGN 101 GEYAWYYEGR NGWWQYDERT ΞRELEDAFSK GKKNTEMLIA GFLYVADLEN 151 MVQYRRNEHG RRRKIKRDII DIPKKGVAGL RLDCDANTVN LARESSADGA 201 DSVSAQSGAS VQPLVSSVRP LTSVDGQLTS PATPSPDAST SLEDSFAHLQ 251 LSGDNTAERS HRGEGEEDHE SPSSGRVPAP DTSIEETESD ASSDSEDVSA 301 VVAQHSLTQQ RLLVSNANQT VPDRSDRSGT DRSVAGGGTV SVSVRSRRPD 351 GQCTVTEV BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_27ol4, frame 1 TREMBL :CEC55A6_1 gene: "C55A6.1"; Caenorhabditis elegans cosmid C55A6, N = 2, Score = 165, P = 4.2e-15 SWISSPROT :YWZ6_CAEEL HYPOTHETICAL 39.3 KD PROTEIN C02B8.6 IN CHROMOSOME X., N = 2, Score = 136, P = 3. le-11 >TREMBL:CEC55A6_1 gene: "C55A6.1"; Caenorhabditis elegans cosmid C55A6 Length = 484 HSPs: Score = 165 (24.8 bits), Expect = 4.2e-15, Sum P(2) = 4.2e-15 Identities = 42/106 (39%), Positives = 61/106 (57%) Query: 75 QEIPEDFLDKPTLLSPEELKAASRGNGEYAWYYEGRN-GWWQYDERTSRELEDAFSKGKK 133 Q +P LD ++ PEE K Y W Y G+N GWW+++ R RE+E+A++ GK Sbjct: 93 QNVPALDLDA-SICDPEERK Y-WIYSGKNQGWWRFEPRNEREIEEAYNAGKC 142 Query: 134 NTEMLIAGFLYVADLENMVQYRRNEHGRRRKIKR DIID-IPKKGVAGL 180 + E++I G YV D +QY R + R +KR D D I KG+AG+ Sbjct: 143 HCEVVICGRPYVIDFHQFLQYPRGVPNQARHVKRVSADDFDGIGVKGLAGI 193 Score = 96 (14.4 bits), Expect = 4.2e-15, Sum P(2) = 4.2e-15 Identities = 19/54 (35%), Positives = 30/54 (55%) Query: 35 ECAICLQTCVHPVSLP-CKHVFCYLCVKGAS —LGKRCALCRQEIPEDFLDKPT 86 EC IC + P ++P C H FC++C+KG +G C +CR I + +P+ Sbjct: 11 ECPICQCKMIVPTTIPACGHKFCFICLKGVYMNDMGG-CPMCRGPIDSNIFAQPS 64 Pedant information for DKFZphtes3_27ol4, frame 1 Report for DKFZphtes3_27ol4.1 [LENGTH] 358 [MW] 38818.90 [pi] 5.17 [HOMOL] TREMBL :CEC55A6_1 gene: "C55A6.1"; Caenorhabditis elegans cosmid C55A6 2e-12 [FUNCAT] 11.04 dna repair (direct repair, base excision repair and nucleotide excision repair) [S. cerevisiae, YCR066w] 3e-04 [FUNCAT] 03.19 recombination and dna repair [S. cerevisiae, YCR066w] 3e-04 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YCR066w] 3e-04 [FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation. palmitylation, farnesylation and processing) [S. cerevisiae, YCR066w] 3e-04 [FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YDR265w] 4e-04 [FUNCAT] 30.19 peroxisomal organization [S. cerevisiae, YDR265w] 4e-04 [BLOCKS] BL00518 Zmc finger, C3HC4 type, proteins [PROSITE] MYRISTYL 2 [PROSITE] AMIDATION 3 [PROSITE] CAMP_PHOSPHO_SITE 1 [PROSITE] CK2_PHOSPHO_SITE 12 [PROSITE] TYR_PHOSPHO_SITE 1 [PROSITE] ZINC_FINGER_C3HC4 1 [PROSITE] PKC_PHOSPHO_SITE 9 [PROSITE] ASN_GLYCOSYLATION 2 [PFAM] Zinc finger, C3HC4 type (RING finger) [KW] Irregular [KW] 3D [KW] LOW COMPLEXITY 19.83 % SEQ MAGCGEIDHSINMLPTNRKANESCSNTAPSLTVPECAICLQTCVHPVSLPCKHVFCYLCV SEG lrmd- TTTTTEETTTEEEETTTEEEEHHHH SEQ KGASWLGKRCALCRQEIPEDFLDKPTLLSPEELKAASRGNGEYAWYYEGRNGWWQYDERT SEG lrmd- HHHHHHCCBTTTTTCBCGGG-CBCC SEQ SRELEDAFSKGKKNTEMLIAGFLYVADLENMVQYRRNEHGRRRKIKRDIIDIPKKGVAGL SEG xxxxxxxxxxxxxxx lrmd- SEQ RLDCDANTVNLARESSADGADSVSAQSGASVQPLVSSVRPLTSVDGQLTSPATPSPDAST SEG xxxxxxxxxxxx lrmd- SEQ SLEDSFAHLQLSGDNTAERSHRGEGEEDHESPΞSGRVPAPDTSIEETESDASSDSEDVSA SEG X xxxxxxxxxxxxxxxxxxxx lrmd- SEQ VVAQHSLTQQRLLVSNANQTVPDRSDRSGTDRSVAGGGTVSVSVRSRRPDGQCTVTEV SEG XXX xxxxxxxxxxxxxxxxxxxx lrmd- Prosite for DKFZphtes3_27ol4.1 PS00001 21->25 ASN_GLYCOΞYLATION PDOC00001 PS00001 318->322 ASN_GLYCOSYLATION PDOC00001 PS00004 132->136 CAMP_PHOSPHO_SITE PDOC00004 PS00005 16->19 PKC_PHOSPHO_SITE PDOC00005 PS00005 120->123 PKC_PHOSPHO_SITE PDOC00005 PS00005 217->220 PKC_PHOSPHO_SITE PDOC00005 PS00005 260->263 PKC_PHOSPHO_SITE PDOC00005 PS00005 274->277 PKC_PHOSPHO_SITE PDOC00005 PS00005 325->328 PKC_PHOSPHO_SITE PDOC00005 PS00005 330->333 PKC_PHOSPHO_SITE PDOC00005 PS00005 343->346 PKC_PHOSPHO_SITE PDOC00005 PS00005 346->349 PKC_PHOSPHO_SITE PDOC00005 PS00006 32->36 CK2_PHOSPHO_SITE PDOC00006 PS00006 89->93 CK2_PHOSPHO_SITE PDOC00006 PΞ00006 120->124 CK2_PHOSPHO_SITE PDOC00006 PS00006 195->199 CK2_PHOSPHO_SITE PDOC00006 PS00006 222->226 CK2_PHOSPHO_SITE PDOC00006 PS00006 240->244 CK2_PHOSPHO_SITE PDOC00006 PS00006 282->286 CK2_PHOSPHO_SITE PDOC00006 PS00006 287->291 CK2_PHOSPHO_ΞITE PDOC00006 PS00006 293->297 CK2_PHOSPHO_SITE PDOC00006 PS00006 320->324 CK2_PHOSPHO_SITE PDOC00006 PS00006 328->332 CK2_PHOSPHO_SITE PDOC00006 PS00006 354->358 CK2_PHOSPHO_SITE PDOC00006 PS00007 98->107 TYR_PHOSPHO_SITE PDOC00007 PS00008 329->335 MYRISTYL PDOC00008 PΞ00008 337->343 MYRISTYL PDOC00008 PS00009 66->70 AMIDATION PDOC00009 PS00009 130->134 AMIDATION PDOC00009 PS00009 159->163 AMIDATION PDOC00009 PS00518 51->61 ZINC FINGER C3HC4 PDOC00449 Pfam for DKFZphtes3_27ol4.1 HMM_NAME Zmc finger, C3HC4 type (RING f ger) HMM *CPICFcTFQlDyPWPFdePmMlPCgHsFCypCIrrW CPmC* C+IC L + P++LPC+H+FCY C++ C +C Query 36 CAIC LQT CVHPVSLPCKHVFCYLCVKGASWLGKRCALC 73 DKFZphtes3_28dl4 group: testes derived DKFZphtes3_28dl4 encodes a novel 97 amino acid protein without similarity to known proteins. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . unknown complete cDNA, complete eds, EST hits Sequenced by GBF Locus: unknown Insert length: 1279 bp Poly A stretch at pos. 1232, no polyadenylation signal found 1 GGAGCTCAGA AGTTGGGCAA AGGTCACAGC AGACTTCCTG AAAAGCAGAC 51 ACTGAGGAAC ACAGTGGAGA GCGGGAGTTC ACAGCGACGC AGCTGAGGAC 101 GACGCAGGAC CTCTCCCAAA GGTGCTGCAG CTCCAGCACC AGGGGCCAGG 151 GCTGCGGCGA CAGCAGCTCA GCAACCCTTG CTGTGCTCAA GTTCTTGGGG 201 ATTCAGAGCT AAGTTCAAAA TTTAGAAACA GTGCCTTAAA GACGGGCAAG 251 AAAACCCGGT GTGGGAGTCT GCTCATCTAT GGTTTGTTAC TGCTCTCGCT 301 TTGATATTCT TAAATTCCTA GGTACCAATG AAAAAGCCAA GTGAACGTGG 351 CAGAGTGAGG AGGAGACAGG AGCGTGTGCA CCTTCCATCT GTGAGAGGCA 401 CACTTCAGTC TGGGTTCAAG ATGCAGAATG GTGCCTACAG CAAAAAAAAA 451 AAAAACACCC TCCTCCCTTC TTTACCATTT GAATGGACAT TTTCCTTACC 501 TGTGATCCCA ACAGAAACAG ATCCAGACCT ATCATGTGAA GTCCACGTTC 551 CAGGATCAGA AGTAACCAGT TTATGGACTG AGCTTACACG GGAAAGTCTA 601 CCCCCGACTC CTTCTGGATA GTAACATACA CAGCTGCATA AAAACGTCTC 651 CAAGGGGACA TACGATGCAT TTGCTTGGTG TCCCAGCCAA GCTCCCCACC 701 GGCGACCTCA CTGTTCCTTA GAGCTCGAGA GCTCGTCTCC TATCAATCAG 751 AGAACCCCAT CAGCTGTGAC CAACAGAGCT GGAGCCCTCT GTGGAGGGAG 801 CTGACCCCAC ACACAGGACA GAGCAGAATC CTGATTATTT TACAAACTGC 851 AAACCTTCTG AGTAAGAAGA CAAAAATATA CATTCCAAGG TATCTGTAAA 901 GTGCTTGGAA GATGCAGACA GCTGCACCGA GGGGCTCTGA TCCATCCACA 951 CGCTGCGCTT TGCTGCGGTC ACACACACGG TCTCAGTCAC GTGATGGTTT 1001 TGCTTTTATT TCTTAAACGG CTGAGTGATA ATCCAGCTAG TGTGCAGTCA 1051 TTTCATACCT TTCAATGGGC GTCACCGCAG TGACGCTGCC CCAGCCCCAT 1101 GCTGAGGGCC GACACAATTC ACGGAACAGA TTCATCATAT TTGGTCTTTA 1151 TGTAAATAAT AAATGTTTTA AAATTGCCTA AATATAAAAA AAAAAAAAAA 1201 AAAAAAAAAA AAAAAAAAAA AAAGGGCGGC CGAAAAAAAA AAAAAAAAAA 1251 AAAAAAAAAA AAAAAAAAAA GGGCGGCCG BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 1 ORF from 328 bp to 618 bp; peptide length: 97 Category: putative protein 1 MKKPSERGRV RRRQERVHLP SVRGTLQSGF KMQNGAYSKK KKNTLLPSLP 51 FEWTFSLPVI PTETDPDLSC EVHVPGSEVT SLWTELTRES LPPTPSG BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_28dl4, frame 1 No Alert BLASTP hits found Pedant information for DKFZphtes3_28dl4, frame 1 Report for DKFZphtes3_28dl4.1 [LENGTH] 97 [MW] 10945.56 [pi] 9.80 [PROSITE] MYRISTYL 2 [PROSITE] CAMP_PHOSPHO_SITE 2 [PROSITE] CK2_PHOSPHO_SITE 2 [PROSITE] PKC_PHOSPHO_SITE 3 [KW] All_Alpha [KW] LOW COMPLEXITY 12.37 SEQ MKKPSERGRVRRRQERVHLPSVRGTLQSGFKMQNGAYSKKKKNTLLPSLPFEWTFSLPVI SEG xxxxxxxxxxxx PRD cccccchhhhhhhhhhhccccccccccccccccccccccccccccccccccccccccccc SEQ PTETDPDLSCEVHVPGSEVTSLWTELTRESLPPTPSG SEG PRD ccccccccceeeecccccchhhhhhhhhhcccccccc Prosite for DKFZphtes3_28dl4.1 PS00004 2->6 CAMP_PHOSPHO_SITE PDOC00004 PS00004 41->45 CAMP_PHOSPHO_SITE PDOC00004 PS00005 5->8 PKC_PHOSPHO_SITE PDOC00005 PS00005 21->24 PKC_PHOSPHO_SITE PDOC00005 PS00005 38->41 PKC_PHOSPHO_SITE PDOC00005 PS00006 62->66 CK2_PHOSPHO_SITE PDOC00006 PS00006 64->68 CK2_PHOSPHO_SITE PDOC00006 PS00008 24->30 MYRISTYL PDOC00008 PS00008 76->82 MYRISTYL PDOC00008 (No Pfam data available for DKFZphtes3_28dl4.1) DKFZphtes3_2all group: testes derived DKFZphtes3_2all encodes a novel 1048 amino acid protein with very weak similarity to mucins. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application m studying the expression profile of testis-specific genes . similarity to mucin complete cDNA, complete eds, EST hits Sequenced by EMBL Locus: unknown Insert length: 4082 bp Poly A stretch at pos. 4060, polyadenylation signal at pos. 4034 1 GAGGACTGCG AGCACAGCGG CGGCCGGGTG GCGGGGGTGA GTGGGGCCAG 51 CGGGGCTGGA CAGCAGCGGG CCCCGGGCGC CGCCGCCGCG ATCCCTCCCC 101 GCGCCCGCCG AGCACATCGC CGCCGCCGAG ATGGGCCCTC CGCGGCACCC 151 CCAGGCCGGC GAGATAGAAG CGGGCGGTGC GGGCGGCGGG CGGCGGCTAC 201 AGGTGGAAAT GAGTTCTCAA CAGTTTCCTC GGTTAGGAGC CCCTTCTACC 251 GGGCTGAGCC AGGCCCCTTC TCAGATTGCA AACAGTGGTT CTGCTGGATT 301 GATAAACCCA GCTGCTACAG TCAATGATGA ATCTGGTCGA GATTCTGAAG 351 TCAGTGCCAG GGAGCACATG AGTTCCAGCA GCTCCCTCCA GTCCCGGGAG 401 GAGAAGCAAG AGCCTGTTGT GGTAAGGCCC TATCCACAGG TGCAGATGTT 451 GTCGACACAC CATGCTGTCG CATCAGCCAC ACCTGTTGCA GTGACAGCCC 501 CGCCAGCACA CCTGACGCCA GCAGTGCCAC TTTCATTTTC GGAGGGACTT 551 ATGAAGCCGC CCCCGAAGCC CACCATGCCT AGCCGTCCCA TTGCTCCTGC 601 TCCACCTTCT ACCCTGTCAC TTCCCCCCAA GGTTCCAGGG CAGGTTACCG 651 TTACCATGGA GAGTAGCATC CCTCAAGCTT CAGCCATTCC TGTGGCAACA 701 ATCAGTGGAC AACAGGGCCA TCCCAGTAAC CTGCATCACA TCATGACTAC 751 AAATGTGCAA ATGTCTATCA TCCGCAGCAA TGCTCCTGGG CCCCCTCTTC 801 ACATTGGAGC TTCTCATTTA CCTCGAGGTG CAGCTGCTGC TGCTGTGATG 851 TCCAGTTCTA AAGTAACCAC AGTCCTGAGG CCGACCTCAC AGCTGCCAAA 901 TGCTGCTACT GCTCAGCCAG CAGTACAGCA CATCATTCAC CAACCAATCC 951 AGTCTCGGCC ACCTGTGACC ACCTCCAATG CCATCCCTCC TGCTGTGGTA 1001 GCAACTGTCT CAGCCACCAG AGCTCAGTCT CCAGTCATCA CTACGACAGC 1051 GGCGCATGCT ACTGATTCAG CACTTAGTAG GCCAACCTTG TCTATCCAGC 1101 ATCCTCCATC TGCAGCAATC AGTATTCAGC GTCCTGCCCA GTCACGAGAT 1151 GTCACAACAA GAATCACACT ACCATCTCAC CCTGCATTAG GGACGCCAAA 1201 ACAGCAGCTT CATACAATGG CTCAGAAAAC AATCTTCAGT ACTGGCACGC 1251 CAGTGGCTGC AGCCACAGTA GCACCTATTT TGGCAACCAA CACCATTCCT 1301 TCAGCGACCA CAGCTGGATC TGTGTCACAC ACGCAAGCTC CCACAAGTAC 1351 CATTGTTACC ATGACAGTAC CCTCCCATTC CTCCCATGCT ACTGCTGTGA 1401 CCACCTCAAA CATCCCAGTC GCCAAGGTGG TGCCCCAGCA GATCACGCAC 1451 ACTTCTCCTC GGATCCAGCC AGACTACCCT GCCGAGAGGA GTAGCCTGAT 1501 TCCCATCTCC GGACATCGGG CCTCTCCCAA TCCTGTGGCC ATGGAAACCC 1551 GAAGTGACAA CAGACCGTCT GTTCCCGTTC AGTTCCAATA TTTTTTGCCA 1601 ACTTACCCCC CTTCTGCATA CCCACTGGCG GCACATACCT ACACCCCAAT 1651 CACCAGTTCC GTGTCCACTA TCCGACAGTA TCCAGTTTCA GCTCAGGCTC 1701 CAAACTCTGC CATCACAGCT CAGACTGGTG TTGGGGTAGC GTCTACCGTC 1751 CACCTAAACC CCATGCAGTT GATGACAGTG GATGCATCGC ATGCTCGACA 1801 TATTCAAGGG ATCCAGCCAG CACCCATCAG TACCCAGGGT ATCCAGCCGG 1851 CCCCCATTGG GACCCCAGGG ATACAGCCTG CACCACTTGG CACACAGGGA 1901 ATTCACTCAG CAACCCCAAT CAACACACAA GGGCTTCAGC CTGCACCTAT 1951 GGGTACTCAG CAGCCTCAGC CTGAAGGAAA GACTTCAGCA GTGGTGTTGG 2001 CAGATGGAGC CACAATTGTG GCCAACCCTA TTAGCAATCC ATTCAGTGCT 2051 GCTCCAGCAG CAACAACCGT GGTGCAGACC CACAGCCAGA GTGCTAGCAC 2101 CAACGCTCCC GCCCAGGGCT CATCGCCACG GCCAAGCATA CTCCGGAAGA 2151 AACCTGCCAC AGATGGTGCC AAACCCAAGT CTGAAATCCA CGTGTCTATG 2201 GCCACTCCGG TCACTGTGTC CATGGAGACT GTATCCAATC AAAATAATGA 2251 TCAGCCTACC ATTGCCGTCC CTCCAACTGC CCAGCAGCCC CCACCGACCA 2301 TTCCAACTAT GATTGCAGCA GCCAGTCCCC CGTCACAACC AGCCGTTGCC 2351 CTTTCAACCA TTCCTGGAGC GGTCCCCATC ACTCCACCCA TCACCACCAT 2401 TGCAGCTGCA CCACCTCCAT CAGTCACTGT GGGTGGCAGT CTTTCCTCCG 2451 TCTTGGGCCC TCCCGTTCCT GAAATTAAAG TGAAAGAAGA AGTAGAACCA 2501 ATGGATATCA TGAGGCCAGT TTCTGCAGTT CCTCCACTGG CTACCAACAC 2551 TGTGTCTCCA TCTCTTGCAT TGCTGGCAAA CAACTTGTCC ATGCCTACAA 2601 GTGACCTACC ACCTGGTGCC TCCCCAAGGA AAAAGCCTCG AAAGCAACAG 2651 CATGTGATCT CAACAGAAGA AGGTGACATG ATGGAGACAA ACAGCACTGA 2701 TGATGAGAAG TCCACTGCCA AGAGTCTTCT GGTGAAGGCT GAGAAGCGCA 2751 AGTCTCCTCC CAAGGAGTAT ATTGATGAGG AAGGTGTGAG ATATGTCCCA 2801 GTGCGTCCAA GACCCCCCAT TACTTTGCTT CGTCACTATC GGAACCCCTG 2851 GAAAGCTGCT TACCACCACT TTCAGAGGTA CAGTGACGTC CGGGTCAAAG 2901 AGGAGAAGAA AGCTATGCTG CAGGAAATAG CTAATCAGAA AGGAGTATCC 2951 TGTCGTGCTC AAGGCTGGAA AGTCCACCTC TGTGCTGCCC AGTTACTACA 3001 GCTGACGAAT CTAGAACATG ATGTCTATGA AAGACTTACT AACCTGCAGG 3051 AAGGGATTAT CCCAAAGAAA AAAGCAGCAA CAGATGATGA TCTCCACCGA 3101 ATAAACGAAC TGATACAGGG AAATATGCAG AGGTGTAAAC TTGTGATGGA 3151 TCAAATCAGT GAAGCCAGAG ACTCCATGCT TAAGGTTTTA GATCATAAAG 3201 ACCGTGTCCT GAAGCTGCTT AACAAGAACG GGACTGTCAA AAAAGTGTCC 3251 AAATTGAAGC GAAAGGAAAA AGTCTAGACC CAGAACAATC AGGAGATTGG 3301 AAGCAAATTT ATGAAGAATG ATGGTGGGGG TGGGGGGAGG GTTTTGGTTT 3351 TTTCCAAAGT GGAACATTGA AATAAAGGAA GTGTTCCTTA GTTCCCGTGT 3401 GAAAGCAGAG GAACCCATGA CATCCAAGGG CGTGAAAGGA TCAGAGCTGA 3451 CTGGACATAG TGAGCTGCCT TCTTGCGTTC GGGTGCACCC CTGTTAAACC 3501 TGATCTGTGT CATAAGTGAC TCCGGATGCA TCAGTGTCCA CCAGTTGGAA 3551 GCAATGACAA GGATGGCTGG CTGGTGTTTT TCAGCCTTCC GGTTTATAGA 3601 CTGTATTTAT CTAGTGGATT CCTGCAGGCC CCATACTGAG CCTGGACTGA 3651 AAGTATCCAC TCGGACCATC TGTTATCTCT CTACACTGAA AATAAAACCT 3701 CTTCCACCCA CCCCATTCGG TTCTTCTGCC TGACCTTCAA ATGCCCATGT 3751 TGGCCTTTTA CAGCAGTGCC ACGGCACCAA GCGAGCTGCC ACATCTCACA 3801 CTCTAAAGGG TTTGAACTAT TAGTTCTTGT CATTTTTTAA AAAAAACCAT 3851 TCCCAAGTGA AATTGTTATA TCGTCTGTCT TGCGTGTGTC AGAACTGGGT 3901 TTTTGTGGAG GTTCAGAGCA GGCAACACCA TAAGTTGCTC TCAGATCCTT 3951 GTTCTGAAGT ACATTCTTGG TTATCTGTAC TTCTGTAGCT GGTGTGATGC 4001 TGTTAATTGT ATGTACCACA CATCTCCAGA CGTTAATAAA GGACTCAAAG 4051 AGGTTTTTGT AAAAAAAAAA AAAAAAAAAA AA BLAST Results No BLAST result Medline entries No Medl e entry Peptide information for frame 2 ORF from 131 bp to 3274 bp; peptide length: 1048 Category: similarity to known protein 1 MGPPRHPQAG EIEAGGAGGG RRLQVEMSSQ QFPRLGAPST GLSQAPSQIA 51 NSGSAGLINP AATVNDESGR DSEVΞAREHM SSSSSLQSRE EKQEPVVVRP 101 YPQVQMLSTH HAVASATPVA VTAPPAHLTP AVPLSFSEGL MKPPPKPTMP 151 SRPIAPAPPS TLSLPPKVPG QVTVTMESSI PQASAIPVAT ISGQQGHPSN 201 LHHIMTTNVQ MSIIRSNAPG PPLHIGASHL PRGAAAAAVM SSΞKVTTVLR 251 PTSQLPNAAT AQPAVQHIIH QPIQSRPPVT TSNAIPPAVV ATVSATRAQS 301 PVITTTAAHA TDSALSRPTL SIQHPPSAAI SIQRPAQSRD VTTRITLPSH 351 PALGTPKQQL HTMAQKTIFS TGTPVAAATV APILATNTIP SATTAGSVSH 401 TQAPTSTIVT MTVPSHSΞHA TAVTTSNIPV AKVVPQQITH TSPRIQPDYP 451 AERSSLIPIΞ GHRASPNPVA METRSDNRPS VPVQFQYFLP TYPPSAYPLA 501 AHTYTPITSS VSTIRQYPVS AQAPNSAITA QTGVGVASTV HLNPMQLMTV 551 DASHARHIQG IQPAPISTQG IQPAPIGTPG IQPAPLGTQG IHSATPINTQ 601 GLQPAPMGTQ QPQPEGKTSA VVLADGATIV ANPISNPFSA APAATTVVQT 651 HSQSASTNAP AQGSSPRPSI LRKKPATDGA KPKSEIHVSM ATPVTVSMET 701 VSNQNNDQPT IAVPPTAQQP PPTIPTMIAA ASPPSQPAVA LSTIPGAVPI 751 TPPITTIAAA PPPSVTVGGS LSSVLGPPVP EIKVKEEVEP MDIMRPVSAV 801 PPLATNTVSP SLALLANNLS MPTSDLPPGA SPRKKPRKQQ HVISTEEGDM 851 METNSTDDEK STAKSLLVKA EKRKSPPKEY IDEEGVRYVP VRPRPPITLL 901 RHYRNPWKAA YHHFQRYSDV RVKEEKKAML QEIANQKGVS CRAQGWKVHL 951 CAAQLLQLTN LEHDVYERLT NLQEGIIPKK KAATDDDLHR INELIQGNMQ 1001 RCKLVMDQIS EARDSMLKVL DHKDRVLKLL NKNGTVKKVS KLKRKEKV BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_2all, frame 2 SWISSPROT :MUC2_HUMAN MUCIN 2 PRECURSOR (INTESTINAL MUCIN 2)., N = 1, Score = 334, P = 2.4e-25 PIR:A43932 mucin 2 precursor, intestinal - human (fragments), N = 1, Score = 321, P = 3.2e-24 TREMBL :D88440_1 product: "high molecular mass nuclear antigen"; Gallus gallus mRNA for high molecular mass nuclear antigen, partial eds., N = 1, Score = 312, P = 8.3e-24 PIR:S48478 glucan 1, 4-alpha-glucosιdase (EC 3.2.1.3) - yeast (Saccharomyces cerevisiae), N = 1, Score = 300, P = 2.1e-22 >SWISSPROT:MUC2_HUMAN MUCIN 2 PRECURSOR (INTESTINAL MUCIN 2) . Length = 5,179 HSPs: Score = 334 (50.1 bits), Expect = 2.4e-25, P = 2.4e-25 Identities = 184/770 (23%), Positives = 263/770 (34%) Query: 96 VVVRPYPQVQMLSTHHAVASATPVAVTAPPAHL-TPAVPLSFSEGLMKPPPKPTMPSRPI 154 V P P T + + T V T P TP + + P P PT P Sbjct: 3471 VTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPT 3530 Query: 155 A-PAPPSTLSLPPKVP-GQVTVTMESSIPQASAIPVATISGQQGHPSNLHHIMTTNVQMS 212 P +T P P G T T + P T +G Q P+ TT V + Sbjct: 3531 TTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQT-PTTTPITTTTTVTPT 3589 Query: 213 IIRSNAPGP PLHIGASHLPRGAAAAA-VMSSSKVTTVLRPTSQLPNAATAQPAVQHI 268 + P P+ + P +++ +TT T T P I Sbjct: 3590 PTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPI 3649 Query: 269 IHQPIQSRPPVTTSNAIPPAVVATVSATRAQSPVITTTAAHATDSALSRPTLSIQHPPSA 328 + P T P T + T +P T T T + T++ P Sbjct: 3650 TTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVT PTPT 3706 Query: 329 AISIQRPAQΞRDVTTRITLPSHPALGTPKQQLHTMAQKT-IFSTGTPVAAAT—VAPILA 385 Q P + TT P+ GT + T + T TP T PI Sbjct: 3707 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 3766 Query: 386 TNTI-PSATTAGSVSHTQAPTSTIVTMT-VPSHSSHATAVTTSNIPVAKVVPQQITHTSP 443 T T+ P+ T G+ + T P +T T+T P+ + T TT V P T T Sbjct: 3767 TTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTP-TPTGT 3825 Query: 444 RIQPDYPAERSSLIPISGHRASPNPVAMETRSDNRPSVPVQFQYFL-PTYPPSAYPLAAH 502 + P ++ + +P P +T + + P+ + PT P+ Sbjct: 3826 QTPTTTPITTTTTVT PTPTPTGTQTPT TTPITTTTTVTPTPTPTG—TQTP 3874 Query: 503 TYTPITSSVS-TIRQYPVSAQAPNSA-ITAQTGVGVASTVHLNPMQLMTVDASHARHIQG 560 T TPIT++ + T P Q P + IT T V T Q T Sbjct: 3875 TTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP—TGTQTPTTTPITTTTTVT 3932 Query: 561 IQPAPISTQGIQPAPIGTPGI QPAPLGTQGIHSATPINTQGL QPAPMGTQQPQ- 613 P P TQ PI T P P GTQ + TPI T P P GTQ P Sbjct: 3933 PTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQ-TPTTTPITTTTTVTPTPTPTGTQTPTT 3991 Query: 614 -PEGKTΞAVVLADGATIVANPISNPFSAAPAAT-TVVQTHSQSASTNAPAQGSSPRPSIL 671 P T+ V T P + P + T T +Q+ +T ++ P+ Sbjct: 3992 TPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPT 4051 Query: 672 RKKPATDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVP PTAQQPPPTIPTMI 728 T P + TP +T + T P PT Q P T P Sbjct: 4052 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 4111 Query: 729 AAASPPSQPAVALSTIPGAVPITPPITTIAAAPPPΞ VTVGGSLSSVLGP-PVPEI 782 p+ T P PIT ττ+ P P+ T + ++ + p p p Sbjct: 4112 TTTVTPTPTPTGTQT-PTTTPITTT-TTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTG 4169 Query: 783 KVKEEVEPMDIMRPVSAVP-PLATNTVSPSLALLANNLSMPTSDLPPGASPRKKPRKQQH 841 P+ V+ P P T T P+ A + TS+ PP +S + R Sbjct: 4170 TQTPTTTPITTTTTVTPTPTPTGTQTGPPTHTΞTAPIAELTTSNPPPESSTPQTSRSTSΞ 4229 Query: 842 VISTEEGDMMET 853 + TE ++ T Sbjct: 4230 PL-TESTTLLST 4240 Score = 328 (49.2 bits), Expect = 1.0e-24, P = 1.0e-24 Identities = 180/745 (24%), Positives = 254/745 (34%) Query: 96 VVVRPYPQVQMLSTHHAVASATPVAVTAPPAHL-TPAVPLSFSEGLMKPPPKPTMPSRPI 154 V P P T + + T V T P TP + + P P PT P Sbjct: 3540 VTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPT 3599 Query: 155 A-PAPPSTLSLPPKVP-GQVTVTMESSIPQASAIPVATISGQQGHPSNLHHIMTTNVQMS 212 P +T P P G T T + P T +G Q P+ TT V + Sbjct: 3600 TTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQT-PTTTPITTTTTVTPT 3658 Query: 213 IIRSNAPGP PLHIGASHLPRGAAAAA-VMSSSKVTTVLRPTSQLPNAATAQPAVQHI 268 + P P+ + P +++ +TT T T P I Sbjct: 3659 PTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPI 3718 Query: 269 IHQPIQSRPPVTTSNAIPPAVVATVSATRAQSPVITTTAAHATDSALSRPTLSIQHPPSA 328 + P T P T + T +P T T T + T++ P Sbjct: 3719 TTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVT PTPT 3775 Query: 329 AISIQRPAQSRDVTTRITLPSHPALGTPKQQLHTMAQKT-IFSTGTPVAAAT—VAPILA 385 Q P + TT P+ GT + T + T TP T PI Sbjct: 3776 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 3835 Query: 386 TNTI-PSATTAGSVSHTQAPTSTIVTMT-VPSHSSHATAVTTSNIPVAKVVPQQITHTSP 443 T T+ P+ T G+ + T P +T T+T P+ + T TT V P T T Sbjct: 3836 TTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTP-TPTGT 3894 Query: 444 RIQPDYPAERSSLIPISGHRASPNPVAMETRSDNRPSVPVQFQYFL-PTYPPSAYPLAAH 502 + P ++ + +P P +T + + P+ + PT P+ Sbjct: 3895 QTPTTTPITTTTTVT PTPTPTGTQTPT TTPITTTTTVTPTPTPTG—TQTP 3943 Query: 503 TYTPITΞSVS-TIRQYPVSAQAPNSA-ITAQTGVGVASTVHLNPMQLMTVDASHARHIQG 560 T TPIT++ + T P Q P + IT T V T Q T Sbjct: 3944 TTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP—TGTQTPTTTPITTTTTVT 4001 Query: 561 IQPAPISTQGIQPAPIGTPGI QPAPLGTQGIHSATPINTQGL QPAPMGTQQPQ- 613 P P TQ PI T P P GTQ + TPI T P P GTQ P Sbjct: 4002 PTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQ-TPTTTPITTTTTVTPTPTPTGTQTPTT 4060 Query: 614 -PEGKTSAVVLADGATIVANPISNPFSAAPAAT-TVVQTHSQSASTNAPAQGSSPRPSIL 671 P T+ V T P + P + T T T +Q+ +T ++ P+ Sbjct: 4061 TPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPT 4120 Query: 672 RKKPATDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVP PTAQQPPPTIPTMI 728 T P + TP +T + T P PT Q P T P Sbjct: 4121 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 4180 Query: 729 AAASPPSQPAVALSTIPGAVPITPPITTIAAA-PPPSVTVGGSLSSVLGPPVPEIKVKEE 787 P+ T P T PI + + PPP + + S P + Sbjct: 4181 TTTVTPTPTPTGTQTGPPTHTSTAPIAELTTSNPPPESSTPQTSRSTSSPLTESTTLLST 4240 Query: 788 VEPMDIMRPVSAVPPLATNTVΞPSLALLANNLSMP—TSDLPPGASPR 833 + P M S PP +T T +P+ + LS P T+ PPG R Sbjct: 4241 LPPAIEM—TSTAPP-STPT-APTTTSGGHTLSPPPSTTTSPPGTPTR 4284 Score = 325 (48.8 bits), Expect = 2.2e-24, P = 2.2e-24 Identities = 186/782 (23%), Positives = 261/782 (33%) Query: 96 VVVRPYPQVQMLSTHHAVASATPVAVTAPPAHL-TPAVPLSFSEGLMKPPPKPTMPΞRPI 154 V P P T + + T V T P TP + + P P PT P Sbjct: 3494 VTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPT 3553 Query: 155 A-PAPPSTLSLPPKVP-GQVTVTMESSIPQASAIPVATISGQQGHPSNLHHIMTTNVQMS 212 P +T P P G T T + P T +G Q P+ TT V + Sbjct: 3554 TTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQT-PTTTPITTTTTVTPT 3612 Query: 213 IIRSNAPGP PLHIGASHLPRGAAAAA-VMSSSKVTTVLRPTSQLPNAATAQPAVQHI 268 + P P+ + P +++ +TT T T P I Sbjct: 3613 PTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPI 3672 Query: 269 IHQPIQSRPPVTTSNAIPPAVVATVΞATRAQSPVITTTAAHATDSALSRPTLSIQHPPSA 328 + P T P T + T +P T T T + T++ P Sbjct: 3673 TTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVT PTPT 3729 Query: 329 AISIQRPAQSRDVTTRITLPSHPALGTPKQQLHTMAQKT-IFSTGTPVAAAT—VAPILA 385 Q P + TT P+ GT + T + T TP T PI Sbjct: 3730 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 3789 Query: 386 TNTI-PSATTAGSVSHTQAPTSTIVTMT-VPSHSSHATAVTTSNIPVAKVVPQQITHTSP 443 T T+ P+ T G+ + T P +T T+T P+ + T TT V P T T Sbjct: 3790 TTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTP-TPTGT 3848 Query: 444 RIQPDYPAERSSLIPISGHRASPNPVAMETRSDNRPSVPVQFQYFL-PTYPPSAYPLAAH 502 + P ++ + +P P +T + + P+ + PT P+ Sbjct: 3849 QTPTTTPITTTTTVT PTPTPTGTQTPT TTPITTTTTVTPTPTPTG—TQTP 3897 Query: 503 TYTPITSSVS-TIRQYPVSAQAPNSA-ITAQTGVGVASTVHLNPMQLMTVDASHARHIQG 560 T TPIT++ + T P Q P + IT T V T Q T Sbjct: 3898 TTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP—TGTQTPTTTPITTTTTVT 3955 Query: 561 IQPAPISTQGIQPAPIGTPGI QPAPLGTQGIHSATPINTQGL QPAPMGTQQPQ- 613 P P TQ PI T P P GTQ + TPI T P P GTQ P Sbjct: 3956 PTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQ-TPTTTPITTTTTVTPTPTPTGTQTPTT 4014 Query: 614 -PEGKTSAVVLADGATIVANPISNPFSAAPAAT-TVVQTHSQSASTNAPAQGSΞPRPSIL 671 P T+ V T P + P + T T T +Q+ +T ++ P+ Sbjct: 4015 TPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPT 4074 Query: 672 RKKPATDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVP PTAQQPPPTIPTMI 728 T P + TP +T + P PT Q P T P Sbjct: 4075 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 4134 Query: 729 AAASPPSQPAVALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSSVLGPPVPEIKVKEEV 788 P+ T P PIT TT P P+ T G+ + P I V Sbjct: 4135 TTTVTPTPTPTGTQT-PTTTPIT TTTTVTPTPTPT—GTQT PTTTPITTTTTV 4184 Query: 789 EPMDIMRPVSAVPPLATNTVSPSLALLANNLSMPTSDLPPGASPRKKPRKQQHVISTEEG 848 P PP T+T +P L +N P S P + P + + + Sbjct: 4185 TPTPTPTGTQTGPPTHTST-APIAELTTSN-PPPESSTPQTSRSTSSPLTESTTLLSTLP 4242 Query: 849 DMMETNSTDDEKSTAKSLLVKAEKRKSPP 877 +E ST + SPP Sbjct: 4243 PAIEMTSTAPPSTPTAPTTTSGGHTLSPP 4271 Score = 324 (48.6 bits), Expect = 2.8e-24, P = 2.8e-24 Identities = 170/717 (23%), Positives = 248/717 (34%) Query: 95 PVVVRPYPQVQMLSTHHAVASATP—VAVTAPPAHLTPAVPLSFSEGLMKPPPKPTMPSR 152 P P P +T + +P T PP TP+ P++ + + P P+ P Sbjct: 1401 PPTTTPSPPPTTTTTLPPTTTPSPPTTTTTTPPPTTTPSPPITTTTTPL-PTTTPSPPIS 1459 Query: 153 PIAPAPPSTLSLPPKVPGQVTVTMESSIPQASAIPVATISGQQGHPSNLHHIMTTNVQMS 212 PP+T PP T S + P T + P I + Sbjct: 1460 TTTTPPPTTTPSPPTTTPSPPTTTPSPPTTTTTTPPPTTTPS PPMTTPITPPASTTT 1516 Query: 213 IIRSNAPGPPLHIGASHLPRGAAAAAVMSSSKVTTVLRPTSQ—LPNAATAQPAVQHIIH 270 + + P PP + P S T + PTS LP T P Sbjct: 1517 LPPTTTPSPPTTTTTTPPP TTTPSPPTTTPITPPTSTTTLPPTTTPSPPPTTTTT 1571 Query: 271 QPIQSRP-PVTTSNAIPPAVVATVSA-TRAQSPVITTTAAHATDSALSRPTLSIQHPPSA 328 P + P P TT+ PP + T T SP TTT + S PT + PP++ Sbjct: 1572 PPPTTTPSPPTTTTPSPPTITTTTPPPTTTPSPPTTTTTTPPPTTTPSPPTTTPITPPTS 1631 Query: 329 AISIQRPAQSRDVTTRITLPSHPALGTPKQQLHTMAQKTIFSTGTPVAAATVAPILATNT 388 ++ T T P P TP T I +T TP T + + T Sbjct: 1632 TTTLPPTTTPSPPPTTTTTP—PPTTTPSPPTTTTPSPPITTTTTPPPTTTPSSPITTTP 1689 Query: 389 IPSATTAGSVSHTQAPTSTIVTMTVPSHSSHATAV-TTSNIPVAKVVPQQITHTSPRIQP 447 P TT + S T P+S I T T PS ++ + TT P P T T + P Sbjct: 1690 SPPTTTMTTPSPTTTPSSPITTTTTPΞSTTTPSPPPTTMTTPSPTTTPSPPTTTMTTLPP 1749 Query: 448 DYPAERSSLIPISGHRASPNPVAMETRSDNRPSVPV-QFQYFLPTYPPSAY-P LA 500 + + P+ P T + P VP+ + +L + P+ + P L Sbjct: 1750 TTTSSPLTTTPLPPSITPPTFSPFSTTTPTTPCVPLCNWTGWLDSGKPNFHKPGGDTELI 1809 Query: 501 AHTYTPITSΞVSTIR—QYP-VSAQAPNSAITAQTGVG-VASTVHLNPMQLMTVDASHAR 556 P ++ + R YP V + VG + P ++ + A Sbjct: 1810 GDVCGPGWAANISCRATMYPDVPIGQLGQTVVCDVSVGLICKNEDQKPGGVIPM-AFCLN 1868 Query: 557 HIQGIQPAPISTQGIQPAPIGTPGIQ-PAPLGTQGIHSATPINTQGLQPAPMGTQQPQ— 613 + +Q TQ P + T + P P T I + T + P P GTQ P Sbjct: 1869 YEINVQCCECVTQ PTTMTTTTTENPTPPTTTPITTTTTVTPT PTPTGTQTPTTT 1922 Query: 614 PEGKTSAVVLADGATIVANPISNPFSAAPAAT-TVVQTHSQSASTNAPAQGSSPRPSILR 672 P T+ V T P + P + T T T +Q+ +T ++ P+ Sbjct: 1923 PITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP 1982 Query: 673 KKPATDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVP PTAQQPPPTIPTMIA 729 T P + TP +T + T P PT Q P T P Sbjct: 1983 TGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTT 2042 Query: 730 AASPPSQPAVALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSSVLGPPVPEIKVKEEVE 789 P+ T P PIT TT P P+ T G+ + P V Sbjct: 2043 TTVTPTPTPTGTQT-PTTTPIT TTTTVTPTPTPT—GTQTPTTTPITTTTTVTPTPT 2096 Query: 790 PMDIMRPVSAVPPLATNTVSPS 811 P P + P T TV+P+ Sbjct: 2097 PTGTQTPTTT-PITTTTTVTPT 2117 Score = 318 (47.7 bits), Expect = 1.2e-23, P = 1.2e-23 Identities = 174/717 (24%), Positives = 243/717 (33%) Query: 96 VVVRPYPQVQMLSTHHAVASATPVAVTAPPAHL-TPAVPLSFSEGLMKPPPKPTMPSRPI 154 V P P T + + T V T P TP + + P P PT P Sb j ct: 2068 VTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPT 2127 Query: 155 A-PAPPSTLSLPPKVP-GQVTVTMESSIPQASAIPVATISGQQGHPSNLHHIMTTNVQMS 212 P +T P P G T T + P T +G Q P+ TT V + Sbjct: 2128 TTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQT-PTTTPITTTTTVTPT 2186 Query: 213 IIRSNAPGP PLHIGASHLPRGAAAAA-VMSSSKVTTVLRPTSQLPNAATAQPAVQHI 268 + P P+ + P +++ +TT T T P I Sbjct: 2187 PTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPI 2246 Query: 269 IHQPIQSRPPVTTSNAIPPAVVATVSATRAQSPVITTTAAHATDSALSRPTLSIQHPPSA 328 + P T P T + T +P T T T + T++ P Sbjct: 2247 TTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVT PTPT 2303 Query: 329 AISIQRPAQΞRDVTTRITLPSHPALGTPKQQLHTMAQKT-IFSTGTPVAAAT—VAPILA 385 Q P + TT P+ GT + T + T TP T PI Sbjct: 2304 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 2363 Query: 386 TNTI-PSATTAGSVSHTQAPTSTIVTMT-VPSHSSHATAVTTΞNIPVAKVVPQQITHTSP 443 T T+ P+ T G+ + T P +T T+T P+ + T TT V P T T Sbjct: 2364 TTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTP-TPTGT 2422 Query: 444 RIQPDYPAERSSLIPISGHRASPNPVAMETRSDNRPSVPVQFQYFL-PTYPPSAYPLAAH 502 + P ++ + +P P +T + + P+ + PT P+ Sbjct: 2423 QTPTTTPITTTTTVT PTPTPTGTQTPT TTPITTTTTVTPTPTPTG—TQTP 2471 Query: 503 TYTPITSSVS-TIRQYPVSAQAPNSA-ITAQTGVGVASTVHLNPMQLMTVDASHARHIQG 560 T TPIT++ + T P Q P + IT T V T Q T Sbjct: 2472 TTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP—TGTQTPTTTPITTTTTVT 2529 Query: 561 IQPAPISTQGIQPAPIGTPGI QPAPLGTQGIHSATPINTQGL QPAPMGTQQPQ- 613 P P TQ PI T P P GTQ + TPI T P P GTQ P Sbjct: 2530 PTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQ-TPTTTPITTTTTVTPTPTPTGTQTPTT 2588 Query: 614 -PEGKTSAVVLADGATIVANPISNPFSAAPAAT-TVVQTHSQSASTNAPAQGSΞPRPSIL 671 P T+ V T P + P + T T T +Q+ +T ++ P+ Sbjct: 2589 TPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPT 2648 Query: 672 RKKPATDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVP PTAQQPPPTIPTMI 728 T P + TP +T + T P PT Q P T P Sbjct: 2649 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 2708 Query: 729 AAASPPSQPAVALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSSVLGPPVPEIKVKEEV 788 P+ T P PIT TT P P+ T G+ + P V Sbjct: 2709 TTTVTPTPTPTGTQT-PTTTPIT TTTTVTPTPTPT—GTQTPTTTPITTTTTVTPTP 2762 Query: 789 EPMDIMRPVSAVPPLATNTVSPS 811 P P + P T TV+P+ Sbjct: 2763 TPTGTQTPTTT-PITTTTTVTPT 2784 Score = 318 (47.7 bits), Expect = 1.2e-23, P = 1.2e-23 Identities = 174/717 (24%), Positives = 243/717 (33%) Query: 96 VVVRPYPQVQMLSTHHAVASATPVAVTAPPAHL-TPAVPLSFSEGLMKPPPKPTMPSRPI 154 V P P T + + T V T P TP + + P P PT P Sbjct: 2206 VTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPT 2265 Query: 155 A-PAPPSTLΞLPPKVP-GQVTVTMESSIPQASAIPVATISGQQGHPSNLHHIMTTNVQMS 212 P +T P P G T T + P T +G Q P+ TT V + Sbjct: 2266 TTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQT-PTTTPITTTTTVTPT 2324 Query: 213 IIRSNAPGP PLHIGASHLPRGAAAAA-VMSSSKVTTVLRPTSQLPNAATAQPAVQHI 268 + P P+ + P +++ +TT T T P I Sbjct: 2325 PTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPI 2384 Query: 269 IHQPIQSRPPVTTSNAIPPAVVATVSATRAQSPVITTTAAHATDSALSRPTLSIQHPPSA 328 + P T P T + T +P T T T + T++ P Sbjct: 2385 TTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVT PTPT 2441 Query: 329 AISIQRPAQSRDVTTRITLPSHPALGTPKQQLHTMAQKT-IFSTGTPVAAAT—VAPILA 385 Q P + TT P+ GT + T + T TP T PI Sb j ct: 2442 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 2501 Query: 386 TNTI-PSATTAGSVSHTQAPTSTIVTMT-VPSHSΞHATAVTTSNIPVAKVVPQQITHTSP 443 T T+ P+ T G+ + T P +T T+T P+ + T TT V P T T Sbjct: 2502 TTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTP-TPTGT 2560 Query: 444 RIQPDYPAERSSLIPISGHRASPNPVAMETRSDNRPSVPVQFQYFL-PTYPPSAYPLAAH 502 + p ++ + +p p +τ + + p+ + PT p+ Sbjct: 2561 QTPTTTPITTTTTVT PTPTPTGTQTPT TTPITTTTTVTPTPTPTG—TQTP 2609 Query: 503 TYTPITSSVS-TIRQYPVSAQAPNSA-ITAQTGVGVASTVHLNPMQLMTVDASHARHIQG 560 T TPIT++ + T P Q P + IT T V T Q T Sbjct: 2610 TTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP—TGTQTPTTTPITTTTTVT 2667 Query: 561 IQPAPISTQGIQPAPIGTPGI QPAPLGTQGIHSATPINTQGL QPAPMGTQQPQ- 613 P P TQ PI T P P GTQ + TPI T P P GTQ P Sbjct: 2668 PTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQ-TPTTTPITTTTTVTPTPTPTGTQTPTT 2726 Query: 614 -PEGKTSAVVLADGATIVANPISNPFSAAPAAT-TVVQTHSQSASTNAPAQGSΞPRPSIL 671 P T+ V T P + P + T T T +Q+ +T ++ P+ Sbjct: 2727 TPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPT 2786 Query: 672 RKKPATDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVP PTAQQPPPTIPTMI 728 T P + TP +T + T P PT Q P T P Sbjct: 2787 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 2846 Query: 729 AAASPPSQPAVALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSSVLGPPVPEIKVKEEV 788 P+ T P PIT TT P P+ T G+ + P V Sbjct: 2847 TTTVTPTPTPTGTQT-PTTTPIT TTTTVTPTPTPT—GTQTPTTTPITTTTTVTPTP 2900 Query: 789 EPMDIMRPVSAVPPLATNTVSPS 811 P P + P T TV+P+ Sbjct: 2901 TPTGTQTPTTT-PITTTTTVTPT 2922 Score = 318 (47.7 bits). Expect = 1.2e-23, P = 1.2e-23 Identities = 174/717 (24%), Positives = 243/717 (33%) Query: 96 VVVRPYPQVQMLSTHHAVASATPVAVTAPPAHL-TPAVPLSFSEGLMKPPPKPTMPSRPI 154 V P P T + + T V T P TP + + P P PT P Sbjct: 2321 VTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPT 2380 Query: 155 A-PAPPSTLSLPPKVP-GQVTVTMESSIPQASAIPVATISGQQGHPSNLHHIMTTNVQMS 212 P +T P P G T T + P T +G Q P+ TT V + Sbjct: 2381 TTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQT-PTTTPITTTTTVTPT 2439 Query: 213 IIRSNAPGP PLHIGASHLPRGAAAAA-VMSSSKVTTVLRPTSQLPNAATAQPAVQHI 268 + P P+ + P +++ +TT T T P I Sbjct: 2440 PTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPI 2499 Query: 269 IHQPIQSRPPVTTSNAIPPAVVATVSATRAQSPVITTTAAHATDSALSRPTLSIQHPPSA 328 + P T P T + T +P T T T + T++ P Sbjct: 2500 TTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVT PTPT 2556 Query: 329 AISIQRPAQSRDVTTRITLPSHPALGTPKQQLHTMAQKT-IFSTGTPVAAAT—VAPILA 385 Q P + TT P+ GT + T + T TP T PI Sbjct: 2557 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 2616 Query: 386 TNTI-PSATTAGΞVSHTQAPTSTIVTMT-VPSHSSHATAVTTSNIPVAKVVPQQITHTSP 443 T T+ P+ T G+ + T P +T T+T P+ + T TT V P T T Sbjct: 2617 TTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTP-TPTGT 2675 Query: 444 RIQPDYPAERSSLIPISGHRASPNPVAMETRSDNRPSVPVQFQYFL-PTYPPSAYPLAAH 502 + P ++ + +P P +T + + P+ + PT P+ Sbjct: 2676 QTPTTTPITTTTTVT PTPTPTGTQTPT TTPITTTTTVTPTPTPTG—TQTP 2724 Query: 503 TYTPITSSVS-TIRQYPVSAQAPNSA-ITAQTGVGVASTVHLNPMQLMTVDASHARHIQG 560 T TPIT++ + T P Q P + IT T V T Q T Sbjct: 2725 TTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP—TGTQTPTTTPITTTTTVT 2782 Query: 561 IQPAPISTQGIQPAPIGTPGI QPAPLGTQGIHSATPINTQGL QPAPMGTQQPQ- 613 P P TQ PI T P P GTQ + TPI T P P GTQ P Sbjct: 2783 PTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQ-TPTTTPITTTTTVTPTPTPTGTQTPTT 2841 Query: 614 -PEGKTSAVVLADGATIVANPISNPFΞAAPAAT-TVVQTHSQSASTNAPAQGSSPRPSIL 671 P T+ V T P + P + T T T +Q+ +T ++ P+ Sbjct: 2842 TPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPT 2901 Query: 672 RKKPATDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVP PTAQQPPPTIPTMI 728 T P + TP +T + T P PT Q P T P Sbjct: 2902 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 2961 Query: 729 AAASPPSQPAVALΞTIPGAVPITPPITTIAAAPPPSVTVGGSLSSVLGPPVPEIKVKEEV 788 P+ T P PIT TT P P+ T G+ + P V Sbjct: 2962 TTTVTPTPTPTGTQT-PTTTPIT TTTTVTPTPTPT—GTQTPTTTPITTTTTVTPTP 3015 Query: 789 EPMDIMRPVSAVPPLATNTVSPS 811 P P + P T TV+P+ Sb j ct: 3016 TPTGTQTPTTT-PITTTTTVTPT 3037 Score = 318 (47.7 bits). Expect = 1.2e-23, P = 1.2e-23 Identities = 174/717 (24%), Positives = 243/717 (33%) Query: 96 VVVRPYPQVQMLSTHHAVASATPVAVTAPPAHL-TPAVPLSFSEGLMKPPPKPTMPSRPI 154 V P P T + + T V T P TP + + P P PT P Sbjct: 2390 VTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPT 2449 Query: 155 A-PAPPSTLSLPPKVP-GQVTVTMESSIPQASAIPVATISGQQGHPSNLHHIMTTNVQMS 212 P +T P P G T T + P T +G Q P+ TT V + Sbjct: 2450 TTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQT-PTTTPITTTTTVTPT 2508 Query: 213 IIRSNAPGP PLHIGASHLPRGAAAAA-VMSSSKVTTVLRPTSQLPNAATAQPAVQHI 268 + P P+ + P +++ +TT T T P I Sbjct: 2509 PTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPI 2568 Query: 269 IHQPIQSRPPVTTSNAIPPAVVATVSATRAQSPVITTTAAHATDSALΞRPTLSIQHPPSA 328 + P T P T + T +P T T T + T++ P Sbjct: 2569 TTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVT PTPT 2625 Query: 329 AISIQRPAQSRDVTTRITLPSHPALGTPKQQLHTMAQKT-IFSTGTPVAAAT—VAPILA 385 Q P + TT P+ GT + T + T TP T PI Sbjct: 2626 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 2685 Query: 386 TNTI-PSATTAGSVSHTQAPTSTIVTMT-VPSHSSHATAVTTSNIPVAKVVPQQITHTSP 443 T τ+ P+ T G+ + T P +T T+T P+ + T TT V P T T Sbjct: 2686 TTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTP-TPTGT 2744 Query: 444 RIQPDYPAERSSLIPISGHRASPNPVAMETRSDNRPSVPVQFQYFL-PTYPPSAYPLAAH 502 + p ++ + +p p +τ + + p+ + PT p+ Sbjct: 2745 QTPTTTPITTTTTVT PTPTPTGTQTPT TTPITTTTTVTPTPTPTG—TQTP 2793 Query: 503 TYTPITSSVS-TIRQYPVSAQAPNSA-ITAQTGVGVASTVHLNPMQLMTVDASHARHIQG 560 T TPIT++ + T P Q P + IT T V T Q T Sbjct: 2794 TTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP—TGTQTPTTTPITTTTTVT 2851 Query: 561 IQPAPISTQGIQPAPIGTPGI QPAPLGTQGIHSATPINTQGL QPAPMGTQQPQ- "613 P P TQ PI T P P GTQ + TPI T P P GTQ P Sbjct: 2852 PTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQ-TPTTTPITTTTTVTPTPTPTGTQTPTT 2910 Query: 614 -PEGKTSAVVLADGATIVANPISNPFSAAPAAT-TVVQTHSQSASTNAPAQGSSPRPSIL 671 P T+ V T P + P + T T +Q+ +T ++ P+ Sbjct: 2911 TPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPT 2970 Query: 672 RKKPATDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVP PTAQQPPPTIPTMI 728 T P + TP +T + T P PT Q P T P Sbjct: 2971 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 3030 Query: 729 AAASPPSQPAVALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSSVLGPPVPEIKVKEEV 788 P+ T P PIT TT P P+ T G+ + P V Sbjct: 3031 TTTVTPTPTPTGTQT-PTTTPIT TTTTVTPTPTPT—GTQTPTTTPITTTTTVTPTP 3084 Query: 789 EPMDIMRPVSAVPPLATNTVSPS 811 P P + P T TV+P+ Sbjct: 3085 TPTGTQTPTTT-PITTTTTVTPT 3106 Score = 318 (47.7 bits), Expect = 1.2e-23, P = 1.2e-23 Identities = 174/717 (24%), Positives = 243/717 (33%) Query: 96 VVVRPYPQVQMLSTHHAVASATPVAVTAPPAHL-TPAVPLSFSEGLMKPPPKPTMPSRPI 154 V P P T + + T V T P TP + + P P PT P Sbjct: 2459 VTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPT 2518 Query: 155 A-PAPPSTLSLPPKVP-GQVTVTMESSIPQASAIPVATISGQQGHPSNLHHIMTTNVQMS 212 P +T P P G T T + P T +G Q P+ TT V + Sbjct: 2519 TTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQT-PTTTPITTTTTVTPT 2577 Query: 213 IIRSNAPGP PLHIGASHLPRGAAAAA-VMSSSKVTTVLRPTSQLPNAATAQPAVQHI 268 + P P+ + P +++ +TT T T P I Sbjct: 2578 PTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPI 2637 Query: 269 IHQPIQSRPPVTTSNAIPPAVVATVSATRAQSPVITTTAAHATDSALSRPTLSIQHPPSA 328 + P T P T + T +P T T T + T++ P Sbjct: 2638 TTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVT PTPT 2694 Query: 329 AISIQRPAQSRDVTTRITLPSHPALGTPKQQLHTMAQKT-IFSTGTPVAAAT—VAPILA 385 Q P + TT P+ GT + T + T TP T PI Sbjct: 2695 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 2754 Query: 386 TNTI-PSATTAGSVSHTQAPTSTIVTMT-VPSHSSHATAVTTSNIPVAK VPQQITHTSP 443 T T+ P+ T G+ + T P +T T+T P+ + T TT V P T T Sbjct: 2755 TTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTP-TPTGT 2813 Query: 444 RIQPDYPAERSΞLIPISGHRASPNPVAMETRSDNRPSVPVQFQYFL-PTYPPSAYPLAAH 502 + p ++ + +p p +τ + + p+ + PT p+ Sbjct: 2814 QTPTTTPITTTTTVT PTPTPTGTQTPT TTPITTTTTVTPTPTPTG--TQTP 2862 Query: 503 TYTPITSSVS-TIRQYPVSAQAPNSA-ITAQTGVGVASTVHLNPMQLMTVDASHARHIQG 560 T TPIT++ + T P Q P + IT T V T Q T Sbjct: 2863 TTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP—TGTQTPTTTPITTTTTVT 2920 Query: 561 IQPAPISTQGIQPAPIGTPGI QPAPLGTQGIHSATPINTQGL QPAPMGTQQPQ- 613 P P TQ PI T P P GTQ + TPI T P P GTQ P Sbjct: 2921 PTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQ-TPTTTPITTTTTVTPTPTPTGTQTPTT 2979 Query: 614 -PEGKTSAVVLADGATIVANPISNPFSAAPAAT-TVVQTHSQSASTNAPAQGSSPRPSIL 671 P T+ V T P + P + T T T +Q+ +T ++ P+ Sbjct: 2980 TPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPT 3039 Query: 672 RKKPATDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVP PTAQQPPPTIPTMI 728 T P + TP +T + T P PT Q P T P Sbjct: 3040 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 3099 Query: 729 AAASPPSQPAVALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSSVLGPPVPEIKVKEEV 788 P+ T P PIT TT P P+ T G+ + P V Sbjct: 3100 TTTVTPTPTPTGTQT-PTTTPIT TTTTVTPTPTPT—GTQTPTTTPITTTTTVTPTP 3153 Query: 789 EPMDIMRPVSAVPPLATNTVSPS 811 P P + P T TV+P+ Sbjct: 3154 TPTGTQTPTTT-PITTTTTVTPT 3175 Score = 318 (47.7 bits), Expect = 1.2e-23, P = 1.2e-23 Identities = 174/717 (24%), Positives = 243/717 (33%) Query: 96 VVVRPYPQVQMLSTHHAVASATPVAVTAPPAHL-TPAVPLSFSEGLMKPPPKPTMPSRPI 154 V P P T + + T V T P TP + + P P PT P Sbjct: 2528 VTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPT 2587 Query: 155 A-PAPPSTLSLPPKVP-GQVTVTMESSIPQASAIPVATISGQQGHPSNLHHIMTTNVQMS 212 P +T P P G T T + P T +G Q P+ TT V + Sbjct: 2588 TTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQT-PTTTPITTTTTVTPT 2646 Query: 213 IIRSNAPGP PLHIGASHLPRGAAAAA-VMSSSKVTTVLRPTSQLPNAATAQPAVQHI 268 + P P+ + P +++ +TT T T P I Sbjct: 2647 PTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPI 2706 Query: 269 IHQPIQSRPPVTTSNAIPPAVVATVSATRAQSPVITTTAAHATDSALSRPTLSIQHPPSA 328 + P T P T + T +P T T T + T++ P Sbjct: 2707 TTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVT PTPT 2763 Query: 329 AISIQRPAQSRDVTTRITLPSHPALGTPKQQLHTMAQKT-IFSTGTPVAAAT—VAPILA 385 Q P + TT P+ GT + T + T TP T PI Sbjct: 2764 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 2823 Query: 386 TNTI-PSATTAGSVSHTQAPTΞTIVTMT-VPSHSSHATAVTTSNIPVAKVVPQQITHTSP 443 T T+ P+ T G+ + T P +T T+T P+ + T TT V P T T Sbjct: 2824 TTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTP-TPTGT 2882 Query: 444 RIQPDYPAERSSLIPISGHRASPNPVAMETRSDNRPSVPVQFQYFL-PTYPPSAYPLAAH 502 + P ++ + +P P +T + + P+ + PT P+ Sbjct: 2883 QTPTTTPITTTTTVT PTPTPTGTQTPT TTPITTTTTVTPTPTPTG—TQTP 2931 Query: 503 TYTPITSSVS-TIRQYPVSAQAPNSA-ITAQTGVGVASTVHLNPMQLMTVDASHARHIQG 560 T TPIT++ + T P Q P + IT T V T Q T Sbjct: 2932 TTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP—TGTQTPTTTPITTTTTVT 2989 Query: 561 IQPAPISTQGIQPAPIGTPGI QPAPLGTQGIHSATPINTQGL QPAPMGTQQPQ- 613 P P TQ PI T P P GTQ + TPI T P P GTQ P Sbjct: 2990 PTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQ-TPTTTPITTTTTVTPTPTPTGTQTPTT 3048 Query: 614 -PEGKTSAVVLADGATIVANPIΞNPFSAAPAAT-TVVQTHSQΞASTNAPAQGSSPRPSIL 671 P T+ V T P + P + T T T +Q+ +T ++ P+ Sbjct: 3049 TPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPT 3108 Query: 672 RKKPATDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVP PTAQQPPPTIPTMI 728 T P + TP +T + T P PT Q P T P Sbjct: 3109 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 3168 Query: 729 AAASPPSQPAVALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSSVLGPPVPEIKVKEEV 788 P+ T P PIT TT P P+ T G+ + P V Sbjct: 3169 TTTVTPTPTPTGTQT-PTTTPIT TTTTVTPTPTPT--GTQTPTTTPITTTTTVTPTP 3222 Query: 789 EPMDIMRPVSAVPPLATNTVSPS 811 P P + P T TV+P+ Sbjct: 3223 TPTGTQTPTTT-PITTTTTVTPT 3244 Score = 318 (47.7 bits), Expect = 1.2e-23, P = 1.2e-23 Identities = 174/717 (24%), Positives = 243/717 (33%) Query: 96 VVVRPYPQVQMLSTHHAVAΞATPVAVTAPPAHL-TPAVPLSFSEGLMKPPPKPTMPΞRPI 154 V P P T + + T V T P TP + + P P PT P Sbjct: 3080 VTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPT 3139 Query: 155 A-PAPPSTLSLPPKVP-GQVTVTMESSIPQASAIPVATISGQQGHPSNLHHIMTTNVQMS 212 P +T P P G T T + P T +G Q P+ TT V + Sbjct: 3140 TTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQT-PTTTPITTTTTVTPT 3198 Query: 213 IIRSNAPGP PLHIGAΞHLPRGAAAAA-VMSSSKVTTVLRPTSQLPNAATAQPAVQHI 2 68 + P P+ + P +++ +TT T T P I Sbjct: 3199 PTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPI 3258 Query: 269 IHQPIQSRPPVTTSNAIPPAVVATVSATRAQSPVITTTAAHATDSALSRPTLSIQHPPSA 328 + P T P T + T +P T T T + T++ P Sbjct: 3259 TTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVT PTPT 3315 Query: 329 AISIQRPAQSRDVTTRITLPSHPALGTPKQQLHTMAQKT-IFSTGTPVAAAT—VAPILA 385 Q P + TT P+ GT + T + T TP T PI Sbjct: 3316 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 3375 Query: 386 TNTI-PSATTAGSVSHTQAPTSTIVTMT-VPSHSSHATAVTTSNIPVAKVVPQQITHTSP 443 T T+ P+ T G+ + T P +T T+T P+ + T TT V P T T Sbjct: 3376 TTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTP-TPTGT 3434 Query: 444 RIQPDYPAERSSLIPISGHRASPNPVAMETRSDNRPSVPVQFQYFL-PTYPPSAYPLAAH 502 + p ++ + +p p +τ + + p+ + PT p+ Sbjct: 3435 QTPTTTPITTTTTVT PTPTPTGTQTPT TTPITTTTTVTPTPTPTG—TQTP 3483 Query: 503 TYTPITSSVS-TIRQYPVSAQAPNΞA-ITAQTGVGVASTVHLNPMQLMTVDASHARHIQG 560 T TPIT++ + T P Q P + IT T V T Q T Sbjct: 3484 TTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP—TGTQTPTTTPITTTTTVT 3541 Query: 561 IQPAPISTQGIQPAPIGTPGI QPAPLGTQGIHSATPINTQGL QPAPMGTQQPQ- 613 P P TQ PI T P P GTQ + TPI T P P GTQ P Sbjct: 3542 PTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQ-TPTTTPITTTTTVTPTPTPTGTQTPTT 3600 Query: 614 -PEGKTSAVVLADGATIVANPISNPFSAAPAAT-TVVQTHSQΞASTNAPAQGSSPRPSIL 671 P T+ V T P + P + T T T +Q+ +T ++ P+ Sbjct: 3601 TPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPT 3660 Query: 672 RKKPATDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVP PTAQQPPPTIPTMI 728 T P + TP +T + T P PT Q P T P Sbjct: 3661 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 3720 Query: 729 AAASPPSQPAVALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSSVLGPPVPEIKVKEEV 788 P+ T P PIT TT P P+ T G+ + P V Sbjct: 3721 TTTVTPTPTPTGTQT-PTTTPIT TTTTVTPTPTPT—GTQTPTTTPITTTTTVTPTP 3774 Query: 789 EPMDIMRPVSAVPPLATNTVSPS 811 P P + P T TV+P+ Sbjct: 3775 TPTGTQTPTTT-PITTTTTVTPT 3796 Score = 313 (47.0 bits), Expect = 4.2e-23, P = 4.2e-23 Identities = 169/695 (24%), Positives = 245/695 (35%) Query: 96 VVVRPYPQVQMLSTHHAVASATPVAVTAPPAHL-TPAVPLSFSEGLMKPPPKPTMPSRPI 154 V P P T + + T V T P TP + + P P PT P Sbjct: 3655 VTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPT 3714 Query: 155 A-PAPPSTLSLPPKVP-GQVTVTMESSIPQASAIPVATISGQQGHPSNLHHIMTTNVQMS 212 P +T P P G T T + P T +G Q P+ TT + Sbjct: 3715 TTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQT-PTTTPITTTTTVTPT 3773 Query: 213 IIRSNAPGP PLHIGASHLPRGAAAAA-VMSSSKVTTVLRPTSQLPNAATAQPAVQHI 268 + P P+ + P +++ +TT T P I Sbjct: 3774 PTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPI 3833 Query: 269 IHQPIQSRPPVTTSNAIPPAVVATVSATRAQSPVITTTAAHATDSALSRPTLSIQHPPSA 328 + P T P T + T +P T T T + T++ P Sbjct: 3834 TTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVT PTPT 3890 Query: 329 AISIQRPAQSRDVTTRITLPΞHPALGTPKQQLHTMAQKT-IFSTGTPVAAAT--VAPILA 385 Q P + TT P+ GT + T + T TP T PI Sbjct: 3891 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 3950 Query: 386 TNTI-PSATTAGSVSHTQAPTSTIVTMT-VPSHSSHATAVTTSNIPVAKVVPQQITHTSP 443 T T+ P+ T G+ + T P +T T+T P+ + T TT V P T T Sbjct: 3951 TTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTP-TPTGT 4009 Query: 444 RIQPDYPAERSSLIPISGHRASPNPVAMETRSDNRPSVPVQFQYFL-PTYPPSAYPLAAH 502 + P ++ + +P P +T + + P+ + PT P+ Sbjct: 4010 QTPTTTPITTTTTVT PTPTPTGTQTPT TTPITTTTTVTPTPTPTG—TQTP 4058 Query: 503 TYTPITSSVS-TIRQYPVSAQAPNSA-ITAQTGVGVASTVHLNPMQLMTVDASHARHIQG 560 T TPIT++ + T P Q P + IT T V T Q T Sbjct: 4059 TTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP—TGTQTPTTTPITTTTTVT 4116 Query: 561 IQPAPISTQGIQPAPIGTPGI QPAPLGTQGIHSATPINTQGL QPAPMGTQQPQP 614 P P TQ PI T P P GTQ + TPI T P P GTQ P Sbjct: 4117 PTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQ-TPTTTPITTTTTVTPTPTPTGTQTPT- 4174 Query: 615 EGKTSAVVLADGATIVANPISNPFSAAPAATTVVQTHSQSASTNAPAQGSSPRPSILRKK 674 T+ + T+ P P T ++ ++N P + S+P+ S Sbjct: 4175 TTPITTT—TTVTPTPTPTGTQTGPPTHTSTAPIAELTTSNPPPESSTPQTSRSTSS 4229 Query: 675 PATDGAKPKSEIH—VSMATPVTVSMETVSNQNNDQPTIAVPP-TAQQPP—PTIPTMIA 729 P T+ S + + M + S T + T++ PP T PP PT T Sbjct: 4230 PLTESTTLLSTLPPAIEMTSTAPPSTPTAPTTTSGGHTLSPPPSTTTSPPGTPTRGTTTG 4289 Query: 730 AASPPSQPAVALSTI PGAVPITPP—ITTIAAAP-PPSVTVGGSLSSVLGPPVPEI 782 ++S P+ V +T P P++ P I T P P ΞV + L+ P E+ Sbjct: 4290 SSSAPTPSTVQTTTTSAWTPTPTPLSTPSIIRTTGLRPYPΞSVLICCVLNDTYYAPGEEV 4349 Score = 279 (41.9 bits), Expect = 1.8e-19, P = 1.8e-19 Identities = 138/540 (25%), Positives = 194/540 (35%) Query: 278 PVTTSNAIPPAVVATVSATRAQSPVITTTAAH ATDSALSRP—TLSIQHPPSAA 329 P+TT+ + P T + T +P+ TTT T + + P T + P Sbjct: 1946 PITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP 2005 Query: 330 ISIQRPAQSRDVTTRITLPSHPALGTPKQQLHTMAQKT-IFSTGTPVAAAT—VAPILAT 386 Q P + TT P+ GT + T + T TP T PI T Sbjct: 2006 TGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTT 2065 Query: 387 NTI-PSATTAGSVSHTQAPTSTIVTMT-VPSHSSHATAVTTSNIPVAKVVPQQITHTSPR 444 T+ P+ T G+ + T P +T T+T P+ + T TT V P T T + Sbjct: 2066 TTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTP-TPTGTQ 2124 Query: 445 IQPDYPAERSSLIPISGHRASPNPVAMETRSDNRPSVPVQFQYFL-PTYPPSAYPLAAHT 503 p ++ + +p p +τ + + p+ + PT p+ T Sbjct: 2125 TPTTTPITTTTTVT PTPTPTGTQTPT TTPITTTTTVTPTPTPTG—TQTPT 2173 Query: 504 YTPITSSVS-TIRQYPVSAQAPNSA-ITAQTGVGVASTVHLNPMQLMTVDASHARHIQGI 561 TPIT++ + T P Q P + IT T V T Q T Sbjct: 2174 TTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP—TGTQTPTTTPITTTTTVTP 2231 Query: 562 QPAPISTQGIQPAPIGTPGI QPAPLGTQGIHSATPINTQGL QPAPMGTQQPQ— 613 P P TQ PI T P P GTQ + TPI T P P GTQ P Sbjct: 2232 TPTPTGTQTPTTTPITTTTTVTPTPTPTGTQ-TPTTTPITTTTTVTPTPTPTGTQTPTTT 2290 Query: 614 PEGKTSAVVLADGATIVANPISNPFSAAPAAT-TVVQTHSQSASTNAPAQGSSPRPSILR 672 P + V T P + P + T T T +Q+ +T ++ P+ Sbjct: 2291 PITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP 2350 Query: 673 KKPATDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVP PTAQQPPPTIPTMIA 729 T P + TP +T + T P PT Q P T P Sbjct: 2351 TGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTT 2410 Query: 730 AAΞPPSQPAVALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSSVLGPPVPEIKVKEEVE 789 P+ T P PIT TT P P+ T G+ + P V Sbjct: 2411 TTVTPTPTPTGTQT-PTTTPIT TTTTVTPTPTPT—GTQTPTTTPITTTTTVTPTPT 2464 Query: 790 PMDIMRPVSAVPPLATNTVSPS 811 P P + P T TV+P+ Sbjct: 2465 PTGTQTPTTT-PITTTTTVTPT 2485 Score = 265 (39.8 bits), Expect = 5.8e-18, P = 5.8e-18 Identities = 179/746 (23%), Positives = 257/746 (34%) Query: 96 VVVRPYPQVQMLSTHHAVASATPVAVTAPPAHL-TPAVPLSFSEGLMKPPPKPTMPSRPI 154 V P P T + + T V T P TP + + P P PT P Sbjct: 3678 VTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPT 3737 Query: 155 A-PAPPSTLSLPPKVP-GQVTVTMESSIPQASAIPVATISGQQGHPSNLHHIMTTNVQMS 212 P +T P P G T T + P T +G Q P+ TT V + Sbjct: 3738 TTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQT-PTTTPITTTTTVTPT 3796 Query: 213 IIRSNAPGP PLHIGASHLPRGAAAAA-VMSSSKVTTVLRPTSQLPNAATAQPAVQHI 268 + P P+ + P +++ +TT T T P I Sbjct: 3797 PTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPI 3856 Query: 269 IHQPIQSRPPVTTSNAIPPAVVATVSATRAQSPVITTTAAHATDSALSRPTLSIQHPPSA 328 + P T P T + T +P T T T + T++ P Sbjct: 3857 TTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVT PTPT 3913 Query: 329 AISIQRPAQSRDVTTRITLPSHPALGTPKQQLHTMAQKT-IFΞTGTPVAAAT—VAPILA 385 Q P + TT P+ GT + T + T TP T PI Sbjct: 3914 PTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITT 3973 Query: 386 TNTI-PSATTAGSVSHTQAPTSTIVTMT-VPSHSΞHATAVTTSNIPVAKVVPQQITHTSP 443 T T+ P+ T G+ + T P +T T+T P+ + T TT V P T T Sbjct: 3974 TTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTP-TPTGT 4032 Query: 444 RIQPDYPAERSSLIPISGHRASPNPVAMETRSDNRPSVPVQFQYFL-PTYPPSAYPLAAH 502 + P ++ + +P P +T + + P+ + PT P+ Sbjct: 4033 QTPTTTPITTTTTVT PTPTPTGTQTPT TTPITTTTTVTPTPTPTG—TQTP 4081 Query: 503 TYTPITSSVS-TIRQYPVSAQAPNSA-ITAQTGVGVASTVHLNPMQLMTVDASHARHIQG 560 T TPIT++ + T P Q P + IT T V T Q T Sbjct: 4082 TTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTP—TGTQTPTTTPITTTTTVT 4139 Query: 561 IQPAPISTQGIQPAPIGTPGI QPAPLGTQGIHSATPINTQGL QPAPMGTQQPQP 614 P P TQ PI T P P GTQ + TPI T P P GTQ P Sbjct: 4140 PTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQ-TPTTTPITTTTTVTPTPTPTGTQTGPP 4198 Query: 615 EGKTSAVVLADGATIVANPISNPFSAAPA ATTVVQTHSQSA-STNAPA—QGSSPRP 668 TS +A+ T +NP P S+ P +T+ T S + ST PA S+ P Sbjct: 4199 T-HTSTAPIAELTT—SNP—PPESSTPQTSRSTSΞPLTESTTLLSTLPPAIEMTSTAPP 4253 Query: 669 SILRKKPATDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVPPTAQQPPPTIPTMI 728 S T G S + +P + ++ PT + T T PT Sbjct: 4254 STPTAPTTTSGGHTLSPPPSTTTSPPGTPTRGTTTGSSSAPTPSTVQTTTTSAWT-PTPT 4312 Query: 729 AAASPPSQPAVALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSΞVLGPPVPEIKVKEEV 788 ++P L P +V I + AP V G+ + E Sbjct: 4313 PLSTPSIIRTTGLRPYPSSVLICCVLNDTYYAPGEEV-YNGTYGDTCYFVNCSLSCTLEF 4371 Query: 789 EPMDIMRPVSAVPPLATNTVSPSLALLANNLSMPTSDLPPGASPRKKPRKQQH 841 S P + +T +PS ++ S PT P P P +Q++ Sbjct: 4372 YNWSCPSTPSPTPTPSKSTPTPΞKP—SSTPSKPTPGTKPPECPDFDPPRQEN 4422 Score = 254 (38.1 bits), Expect = 8.7e-17, P = 8.7e-17 Identities = 167/697 (23%), Positives = 245/697 (35%) Query: 115 ΞATPVAVTAPPAHLTPAVPLSFSEGLMKPPPK--PTMPSR-PIAPAPPSTLSLPPKV-PG 170 S + T PP TP+ P + + PPP P+ P+ PI P P ST +LPP P Sbjct: 1587 SPPTITTTTPPPTTTPSPPTTTTT TPPPTTTPSPPTTTPITP-PTSTTTLPPTTTPS 1642 Query: 171 QVTVTMESSIPQASAIPVATISGQQGHPSNLHHIMTTNVQMSIIRSNAPGPPLHIGASHL 230 T + P + P T + + TT I + P PP + Sbjct: 1643 PPPTTTTTPPPTTTPSPPTTTTPSPPITTTTTPPPTTTPSSPI—TTTPSPPTTTMTTPS 1700 Query: 231 PRGAAAAAVMSSSKVTTVLRPTSQLPNAATAQPAVQHIIHQPIQS-RPPVTTSNAIPPAV 289 P SS +TT P+S + P P + PP TT +PP Sbjct: 1701 P TTTPSSPITTTTTPSS TTTPSPPPTTMTTPSPTTTPSPPTTTMTTLPPTT 1751 Query: 290 VATVSATRAQSPVITT-TAAHATDSALSRPTLΞIQH PPSAAISIQRPAQSRDVTTR 344 ++ T P IT T + + + + P + + + S + +P ++ Sbjct: 1752 TSSPLTTTPLPPSITPPTFSPFSTTTPTTPCVPLCNWTGWLDSGKPNFHKPGGDTELIGD 1811 Query: 345 ITLPSHPALGTPKQQLHTMAQKTIFΞTGTPVAAATVAPILATN TIPSATTAGS 397 + P A + + ++ I G V ++ N IP A Sbjct: 1812 VCGPGWAANISCRATMYP—DVPIGQLGQTVVCDVSVGLICKNEDQKPGGVIPMAFCLNY 1869 Query: 398 VSHTQAPTSTI—VTMTVPSHSSHATAVTTSNIPVAKVVPQQITHTΞPRIQPDYPAERSS 455 + Q TMT + + + T TT+ I V T T + P ++ Sbjct: 1870 EINVQCCECVTQPTTMTTTT-TENPTPPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTT 1928 Query: 456 LIPISGHRASPNPVAMETRSDNRPSVPVQFQYFL-PTYPPSAYPLAAHTYTPITSSVS-T 513 + +P P +T + + P+ + PT P+ T TPIT++ + T Sbjct: 1929 TVT PTPTPTGTQTPT TTPITTTTTVTPTPTPTG— QTPTTTPITTTTTVT 1977 Query: 514 IRQYPVSAQAPNSA-ITAQTGVGVASTVHLNPMQLMTVDASHARHIQGIQPAPISTQGIQ 572 P Q P + IT T V T Q T P P TQ Sbjct: 1978 PTPTPTGTQTPTTTPITTTTTVTPTPTP— GTQTPTTTPITTTTTVTPTPTPTGTQTPT 2035 Query: 573 PAPIGTPGI QPAPLGTQGIHSATPINTQGL QPAPMGTQQPQ—PEGKTSAVVLA 624 PI T P P GTQ + TPI T P P GTQ P P T+ V Sbjct: 2036 TTPITTTTTVTPTPTPTGTQ-TPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPT 2094 Query: 625 DGATIVANPISNPFSAAPAAT-TVVQTHSQSASTNAPAQGSSPRPSILRKKPATDGAKPK 683 T P + P + T T T +Q+ +T ++ P+ T P Sbjct: 2095 PTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPI 2154 Query: 684 SEIHVSMATPVTVSMETVSNQNNDQPTIAVP PTAQQPPPTIPTMIAAASPPSQPAVA 740 + TP +T + T P PT Q P T P P+ Sbjct: 2155 TTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTG 2214 Query: 741 LSTIPGAVPITPPITTIAAAPPPSVTVGGΞLSSVLGPPVPEIKVKEEVEPMDIMRPVSAV 800 T P PIT TT P P+ T G+ + P V P P + Sbjct: 2215 TQT-PTTTPIT TTTTVTPTPTPT—GTQTPTTTPITTTTTVTPTPTPTGTQTPTTT- 2267 Query: 801 PPLATNTVSPS 811 P T TV+P+ Sbjct: 2268 PITTTTTVTPT 2278 Score = 243 (36.5 bits), Expect = 1.3e-15, P = 1.3e-15 Identities = 110/406 (27%), Positives = 154/406 (37%) Query: 121 VTAP-PAHLTPAVPLSFSEGLMKPPPKPTMPSRPIAPAPPSTLSLPPKVPGQVTVTMESS 179 +T P P TP+ P + + L P P+ P+ PP+T PP T + ++ Sbjct: 1396 ITTPSPPTTTPSPPPTTTTTL-PPTTTPSPPTTTTTTPPPTTTPSPPITT—TTTPLPTT 1452 Query: 180 IPQASAIPVATISGQQGHPSNLHHIMTTNVQMSIIRSNAPGPPLHIGASHLPRGAAAAAV 239 P P++T + P+ TT + P PP + P Sbjct: 1453 TPSP PISTTTTPP—PTTTPSPPTTTPSPP TTTPSPPTTTTTTPPP TT 1498 Query: 240 MΞSSKVTTVLRP TSQLPNAATAQPAVQHIIHQPIQSRP-PVTTSNAIPPAVVATVSA 295 S +TT + P T+ LP T P P + P P TT+ PP T+ Sbjct: 1499 TPSPPMTTPITPPASTTTLPPTTTPSPPTTTTTTPPPTTTPSPPTTTPITPPTSTTTLPP 1558 Query: 296 TRAQSPVITTTAAHATDSALSRPTLSIQHPPSAAISIQRPAQSRDV-TTRITLPSHPALG 354 T SP TTT + S PT + PP+ + P + TT T P P Sbjct: 1559 TTTPSPPPTTTTTPPPTTTPSPPTTTTPSPPTITTTTPPPTTTPSPPTTTTTTP—PPTT 1616 Query: 355 TPKQQLHTMAQKTIFSTGTPVAAATVAPILATNTIPSATTAGSVSHTQAPTSTIVTMTVP 414 TP T +T P T +P T T P TT S T P+ I T T P Sbjct: 1617 TPSPPTTTPITPPTSTTTLP-PTTTPSPPPTTTTTPPPTTTPSPPTTTTPSPPITTTTTP 1675 Query: 415 ΞHSSHATA-VTTSNIPVAKVVPQQITHTSPRIQPDYPAERSSLIPISGHRASPNPVAMET 473 ++ ++ +TT+ P + T SP P P ++ P S SP P M T Sbjct: 1676 PPTTTPSSPITTTPSPPTTTM TTPSPTTTPSSPITTTTT-PSSTTTPSPPPTTMTT 1730 Query: 474 RSDNR-PSVPVQFQYFLPTYPPSAYPLAAHTYTPITSSVSTIRQYPVSAQAPNS 526 S PS P LP S+ PL T TP+ S++ P S P + Sbjct: 1731 PSPTTTPSPPTTTMTTLPPTTTSS-PL TTTPLPPSITPPTFSPFSTTTPTT 1780 Score = 189 (28.4 bits). Expect = 8.0e-09, P = 8.0e-09 Identities = 92/374 (24%), Positives = 133/374 (35%) Query: 439 THTSPRIQPDYPAERSSLIPISGHRASPNPVAMETRSDNRPSVPVQFQYF-LPTYPPSAY 497 T + P P P ++ +P + + P PS P+ LPT PS Sbjct: 1398 TPSPPTTTPSPPPTTTTTLPPTTTPSPPTTTTTTPPPTTTPSPPITTTTTPLPTTTPSP- 1456 Query: 498 PLAAHTYTPITSSVSTIRQYPVSAQAPNSAITAQTGVGVASTVHLNPMQL-MTVDASHAR 556 P++ T P T++ S P S T T +T PM +T AS Sbjct: 1457 PISTTTTPPPTTTPΞPPTTTPSPPTTTPSPPTTTTTTPPPTTTPSPPMTTPITPPASTTT 1516 Query: 557 HIQGIQPAPISTQGIQPAPIGTPGIQPAPLGTQGIHΞATPINTQGLQPAPMGTQQPQPEG 616 P+P +T P P TP +P T I P +T L P T P P Sbjct: 1517 LPPTTTPSPPTTTTTTPPPTTTP SPPTTTPI—TPPTSTTTLPP TTTPSPPP 1566 Query: 617 KTSAVVLADGATIVANPISNPFSAAPAATTVVQTHΞQSAΞTNAP—AQGSSPRPSILRKK 674 T+ T +P P + P+ T+ T +T +P ++P P+ Sbjct: 1567 TTTTT PPPTTTPΞP PTTTTPSPPTITTTTPPPTTTPSPPTTTTTTPPPTTTPSP 1620 Query: 675 PATDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAV-PPTAQQPPPTIPTMIAA—A 731 P T P + + P T + PT PPT P P I T Sbjct: 1621 PTTTPITPPTS— TTLPPTTTPSPPPTTTTTPPPTTTPSPPTTTTPSPPITTTTTPPPT 1678 Query: 732 SPPSQPAVALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSSVLGPPV PEIKVK 785 + PS P + P TP TT ++P + T S ++ PP P Sb j ct: 1679 TTPSSPITTTPSPPTTTMTTPSPTTTPSSPITTTTTPSSTTTPSPPPTTMTTPSPTTTPS 1738 Query: 786 EEVEPMDIMRPVSAVPPLATNTVSPSL 812 M + P + PL T + PS+ Sb j ct: 1739 PPTTTMTTLPPTTTSSPLTTTPLPPSI 1765 Score = 185 (27.8 bits), Expect = 1.6e-09, P = 1.6e-09 Identities = 71/270 (26%), Positives = 99/270 (36%) Query: 563 PAPIΞTQGIQPAPIGTPGIQPAPLGTQGIHSATP INTQGLQPAPMGTQQPQ PEG 616 P+P +T P P TP P T + + TP I+T P P T P P Sbjct: 1422 PSPPTTTTTTPPPTTTPS-PPITTTTTPLPTTTPSPPISTT-TTPPPTTTPSPPTTTPSP 1479 Query: 617 KTSAVVLADGATIVANPISNPFSAAPAATTVVQTHSQSASTNAPAQGSSPRPSILRKKPA 676 T+ T P + P +P TT + T S +T P SP + P Sbjct: 1480 PTTTPSPPTTTTTTPPPTTTP SPPMTTPI-TPPASTTTLPPTTTPSPPTTTTTTPPP 1535 Query: 677 TDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVPPTAQQPPPTIPTMIAAASPPSQ 736 T P + TP+T T + P+ P T PPPT + PS Sbjct: 1536 TTTPSPPT TTPITPPTSTTTLPPTTTPS-PPPTTTTTPPPTTTPSPPTTTTPSP 1588 Query: 737 PAVALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSΞVLGPPVPEIKVKEEVEPMDIMRP 796 P + +T P +PP TT PPP+ T ++ + PP + P P Sbjct: 1589 PTITTTTPPPTTTPSPPTTT-TTTPPPTTTPSPPTTTPITPPTSTTTLPPTTTPSP—PP 1645 Query: 797 VSAVPPLATNTVSPSLALLANNLSMPTSDLPPGASP 832 + P T T SP + T+ PP +P Sbjct: 1646 TTTTTPPPTTTPSPPTTTTPSPPITTTTTPPPTTTP 1681 Score = 183 (27.5 bits), Expect = 3.4e-09, P = 3.4e-09 Identities = 91/390 (23%), Positives = 139/390 (35%) Query: 326 PSAAISIQRPAQSRDVTTR-ITLPSHPALGTPKQQLHTMAQKTIFSTGTPVAAATVAPIL 384 PS + P + T T PS P T T I +T TP+ T +P + Sbjct: 1399 PSPPTTTPSPPPTTTTTLPPTTTPSPPTTTTTTPPPTTTPSPPITTTTTPLPTTTPSPPI 1458 Query: 385 ATNTIPSATTAGSVSHTQAPTSTIVTMTVPSHSSHATAVTTSNIP—VAKVVPQQITHTS 442 +T T P TT S T P+ T + P+ ++ TT+ P + P T T Sbjct: 1459 STTTTPPPTTTPSPP-TTTPSPPTTTPSPPTTTTTTPPPTTTPSPPMTTPITPPASTTTL 1517 Query: 443 PRIQPDYPAERSSLIPISGHRASP NPVAMETRSDNRP--SVPVQFQYFLPTYPPSAY 497 P P ++ P SP P+ T + P + P T PP+ Sbjct: 1518 PPTTTPSPPTTTTTTPPPTTTPSPPTTTPITPPTΞTTTLPPTTTPSPPPTTTTTPPPTTT 1577 Query: 498 PLAAHTYTPITSSVSTIRQYPVSAQAPNSAITAQTGVGVASTVHLNPMQL-MTVDASHAR 556 P T TP +++T P + +P T T +T P +τ s Sbjct: 1578 PSPPTTTTPSPPTITTTTPPPTTTPΞPP TTTTTTPPPTTTPSPPTTTPITPPTSTTT 1634 Query: 557 HIQGIQPAPISTQGIQPAPIGTPGIQPAPLGTQGIHSATPINTQGLQPAPMGTQQPQPEG 616 P+P T P P TP P P T T T P P Sbjct: 1635 LPPTTTPSPPPTTTTTPPPTTTPS—P-PTTTTPSPPITTTTTPPPTTTPSSPITTTPSP 1691 Query: 617 KTSAVVLADGATIVANPISNPFSAAPAATTVVQTHSQSASTNAPAQGSSPRPSILRKKPA 676 T+ + T ++PI+ + P++TT + +T +P SP + + P Sbjct: 1692 PTTTMTTPSPTTTPSSPITT—TTTPSSTTTPSPPPTTMTTPΞPTTTPSPPTTTMTTLPP 1749 Query: 677 TDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVPP 715 T + P + + P +++ T S + PT P Sbjct: 1750 TTTSSPLT TTPLPPSITPPTFSPFSTTTPTTPCVP 1784 Score = 176 (26.4 bits), Expect = 1.8e-07, P = 1.8e-07 Identities = 101/402 (25%), Positives = 142/402 (35%) Query: 345 ITLPSHPALGTPKQQLHTMAQKTIFSTGTPVAAATVAPILATNTIPSATTAGΞVSHTQAP 404 IT PS P TP T +T +P T P T P TT + T P Sbjct: 1396 ITTPSPPTT-TPSPPPTTTTTLPPTTTPSPPTTTTTTPPPTTTPSPPITTTTTPLPTTTP 1454 Query: 405 TSTIVTMTVPSHSSHATAVTTS-NIPVAKVVPQQITHTSPRIQPDYPAERΞSLIPISGHR 463 + I T T P ++ + TT+ + P P T T+P P PI+ Sbjct: 1455 SPPISTTTTPPPTTTPSPPTTTPSPPTTTPSPPTTTTTTP—PPTTTPSPPMTTPITPP- 1511 Query: 464 ASPNPVAMETRSDNRPSVPVQFQYFLPTYPPSAYPLAAHTYTPITSSVSTIRQYPVSAQA 523 AS + T PS P T PP+ P + T TPIT ST P + + Sbjct: 1512 ASTTTLPPTTT PΞPPTTTT TTPPPTTTP-SPPTTTPITPPTΞTTTLPPTTTPS 1563 Query: 524 PNSAITAQ TGVGVASTVHLNPMQLMTVDASHARHIQGIQPAPISTQGIQPAPIGTP 579 P T T +T +P + T P+P +T P P TP Sbjct: 1564 PPPTTTTTPPPTTTPSPPTTTTPSPPTITTTTPPPTT TPSPPTTTTTTPPPTTTP 1618 Query: 580 G IQPAPLGTQGIHSAT PINTQGLQPAPMGTQQPQPEGKTSAVVLADGATIV 630 I P P T + T P T P P T P S + Sbjct: 1619 SPPTTTPITP-PTSTTTLPPTTTPSPPPTTTTTPPPTTTPΞPPTTTTPΞPPITTTTTPPP 1677 Query: 631 ANPISNPFSAAPAA-TTVVQTHSQSASTNAP-AQGSSPRPSILRKKPATDGAKPKSEIHV 688 S+P + P+ TT + T S + + ++P ++P + P T P Sbjct: 1678 TTTPΞSPITTTPSPPTTTMTTPSPTTTPSSPITTTTTPSSTTTPSPPPTTMTTPSP T 1734 Query: 689 SMATPVTVSMETVSNQNNDQPTIAVPPTAQQPPPTIPTMIAAASPPSQPAVALSTIPG 746 + +P T +M T+ P P PPT + + P+ P V L G Sbjct: 1735 TTPSPPTTTMTTLPPTTTSSPLTTTPLPPSITPPTFSPF--STTTPTTPCVPLCNWTG 1790 Score = 168 (25.2 bits), Expect = 9.3e-08, P = 9.3e-08 Identities = 89/387 (22%), Positives = 133/387 (34%) Query: 448 DYPAERΞSLIPISGHRASPNPVAMETRSDNRPSVPVQFQYFLPTYPPSAYPLAAHTYTPI 507 DY + P+ +P+P T + + P P PT PS P T P Sbjct: 1381 DYKIRVNCCWPMDKCITTPSP PTTTPSPP—PTTTTTLPPTTTPSP-PTTTTTTPPP 1434 Query: 508 TSSVS TIRQYPVSAQAPNSAITAQTGVGVASTVHLNPMQLMTVDASHARHIQGIQPA 564 T++ S T P+ P+ 1+ T +T P T + P+ Sbjct: 1435 TTTPSPPITTTTTPLPTTTPSPPIΞTTTTPPPTTT PSPPTTTPSPPTT TPS 1485 Query: 565 PISTQGIQPAPIGTPGI-QPAPLGTQGIHSATPINTQGLQPAPMGTQQPQ PEGKTSA 620 P +T P P TP P+ + P T P T P P T+ Sbjct: 1486 PPTTTTTTPPPTTTPSPPMTTPITPPASTTTLPPTTTPSPPTTTTTTPPPTTTPSPPTTT 1545 Query: 621 VVLADGATIVANPISNPFΞAAPAATTVVQTHSQSA-ΞTNAPAQGS ΞPRPSILRKKP 675 + +T P + P TT T + S +T P+ + +P P+ P Sbjct: 1546 PITPPTSTTTLPPTTTPSPPPTTTTTPPPTTTPSPPTTTTPSPPTITTTTPPPTTTPSPP 1605 Query: 676 ATDGAKPKSEIHVS—MATPVTVSMETVSNQNNDQPTIAVPPTAQQPPPTIPTMIAAASP 733 T P Ξ TP+T T + P+ P T PPPT + Sbjct: 1606 TTTTTTPPPTTTPSPPTTTPITPPTSTTTLPPTTTPS-PPPTTTTTPPPTTTPSPPTTTT 1664 Query: 734 PSQPAVALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSSVLGP PVPEIKVKEEVE 789 PS P +T P + PITT + P ++T ++ P P Sbjct: 1665 PSPPITTTTTPPPTTTPSSPITTTPSPPTTTMTTPSPTTTPSSPITTTTTPSSTTTPSPP 1724 Query: 790 PMDIMRPVSAVPPLATNTVSPSLALLANNLSMPTSDLPPGASP 832 P + P P T +L + + T+ LPP +P Sbjct: 1725 PTTMTTPΞPTTTPSPPTTTMTTLPPTTTSSPLTTTPLPPSITP 1767 Score = 154 (23.1 bits), Expect = 2.7e-06, P = 2.7e-06 Identities = 70/277 (25%), Positives = 92/277 (33%) Query: 565 PISTQGIQPAPIGTPGIQPAPLGTQGIHSATPINTQGLQPAPMGTQQPQPEGKTSAVVLA 624 PIST P P TP P P T + TP P T P P T + Sbjct: 1457 PISTT-TTPPPTTTPS—P-PTTTPSPPTTTPSPPTTTTTTPPPTTTPSPPMTTP—ITP 1510 Query: 625 DGATIVANPISNPFSAAPAATTVVQTHSQSASTNAP AQGSSPRPSILRKKPATDGA 680 +T P + P TT T + S T P ++ P+ P T Sbjct: 1511 PASTTTLPPTTTPSPPTTTTTTPPPTTTPSPPTTTPITPPTSTTTLPPTTTPSPPPTTTT 1570 Query: 681 KPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVPPTAQQ—PPPTIPTMIAAASPPSQPA 738 P S T T S T++ T PPT PPPT T + P P Sbjct: 1571 TPPPTTTPSPPTTTTPSPPTITTTTPPPTTTPSPPTTTTTTPPPTT-TPSPPTTTPITPP 1629 Query: 739 VALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSSVLGPPVPEIKVKEEVEPMDIMRPVS 798 + +T+P +PP TT PPP+ T ++ PP+ + Sbjct: 1630 TSTTTLPPTTTPSPPPTT-TTTPPPTTTPSPPTTTTPSPPITTTTTPPPTTTPSSPITTT 1688 Query: 799 AVPPLATNTV SPSLALLANNL--SMPTSDLPPGASPRKKP 836 pp T T +PS + S T PP P Sbjct: 1689 PSPPTTTMTTPSPTTTPSSPITTTTTPSSTTTPSPPPTTMTTPSP 1733 Score = 148 (22.2 bits), Expect = l.le-05, P = l.le-05 Identities = 62/254 (24%), Positives = 89/254 (35%) Query: 583 PAPLGTQGIHSATPINTQGLQPAPMGTQQPQPEGKTSAV VLADGATIVANPISNP 637 P+P T S P T L P T P P T+ + T P+ Sbjct: 1399 PSPPTTTP—SPPPTTTTTLPP TTTPSPPTTTTTTPPPTTTPSPPITTTTTPLPTT 1452 Query: 638 FSAAPAATTVVQTHSQSASTNAPAQGSSPRPSILRKKPATDGAKPKΞEIHVS—MATPVT 695 + P +TT T + + + P SP P+ P T P S M TP+T Sbjct: 1453 TPSPPISTTT—TPPPTTTPSPPTTTPSP-PTTTPSPPTTTTTTPPPTTTPSPPMTTPIT 1509 Query: 696 VSMETVSNQNNDQPTIAVPPTAQQPPPTIPTMIAAASPPSQPAVALSTIPGAVPITPPIT 755 T + P+ T PP T P+ + P P + +T+P +PP T Sbjct: 1510 PPASTTTLPPTTTPSPPTTTTTTPPPTTTPS—PPTTTPITPPTSTTTLPPTTTPSPPPT 1567 Query: 756 TIAAAPPPSVTVGGSLSSVLGPPVPEIKVKEEVEPMDIMRPVSAVPPLATNTVSPSLALL 815 T PPP+ T ++ PP + PP T P+ + Sbjct: 1568 T-TTTPPPTTTPSPPTTTTPSPPTITTTTPPPTTTPSPPTTTTTTPPPTTTPSPPTTTPI 1626 Query: 816 ANNLSMPTSDLPPGASPRKKP 836 S T+ LPP +P P Sb j ct: 1627 TPPTS—TTTLPPTTTPSPPP 1645 Score = 131 (19.7 bits), Expect = 1.2e-03, P = 1.2e-03 Identities = 112/492 (22%), Positives = 174/492 (35%) Query: 96 VVVRPYPQVQMLSTHHAVASATPVAVTAPPAHL-TPAVPLSFSEGLMKPPPKPTMPSRPI 154 V P P T + + T V T P TP + + P P PT P Sbjct: 3977 VTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPT 4036 Query: 155 A-PAPPSTLSLPPKVP-GQVTVTMESSIPQASAIPVATISGQQGHPSNLHHIMTTNVQMΞ 212 P +T P P G T T + P T +G Q P+ TT V + Sbjct: 4037 TTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQT-PTTTPITTTTTVTPT 4095 Query: 213 IIRSNAPGP PLHIGASHLPRGAAAAA-VMSSSKVTTVLRPTSQLPNAATAQPAVQHI 268 + P P+ + P +++ +TT T T P I Sbjct: 4096 PTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTPTTTPI 4155 Query: 269 IHQPIQSRPPVTTSNAIPPA—VVATVΞATRAQSPVITTTA—AHATDSALΞRPTLSIQH 324 + P T P + T + T +P T T H + + ++ T Ξ Sbjct: 4156 TTTTTVTPTPTPTGTQTPTTTPITTTTTVTPTPTPTGTQTGPPTHTSTAPIAELTTSNPP 4215 Query: 325 PPSAAISIQRPAQS—RDVTTRI-TLPSHPALGTPKQQLHTMAQKTIFSTGTPVAAATVA 381 P S+ R S + TT + TLP PA+ + T T + T T++ Sbjct: 4216 PESSTPQTΞRSTSSPLTESTTLLSTLP—PAI EMTSTAPPSTPTAPTTTSGGHTLS 4269 Query: 382 PILATNTIPSAT-TAGSVS-HTQAPTSTIVTMTVPSHSΞHATAVTTSNIPVAKVVPQQIT 439 P +T T P T T G+ + + APT + V T S A T + P++ P I Sbjct: 4270 PPPSTTTSPPGTPTRGTTTGSSSAPTPSTVQTTTTS AWTPTPTPLS—TPSIIR 4321 Query: 440 HTSPRIQPDYPAERSSLIPISGHRASPNP-VAMETRSDN RPSVPVQFQYFLPTYP- 493 T ++P YP+ ++ +P V T D S+ +++ + P Sbjct: 4322 TTG—LRP-YPSSVLICCVLNDTYYAPGEEVYNGTYGDTCYFVNCSLSCTLEFYNWSCPS 4378 Query: 494 -PSAYPLAAHTYTPITSSVSTIRQYPVSAQAPNSAITAQTGVGVASTVHLNPMQLMTVDA 552 PS P + + TP S S+ P P T L + T Sbjct: 4379 TPSPTPTPSKS-TPTPSKPSSTPSKPTPGTKPPECPDFDPPRQENETWWLCDCFMATCKY 4437 Query: 553 SHARHIQGIQ PAPISTQGIQPAPIGTP 579 ++ I ++ P P + G+QP + P Sbjct: 4438 NNTVEIVKVECEPPPMPTCSNGLQPVRVEDP 4468 Score = 117 (17.6 bits), Expect = 1.8e-02, P = 1.8e-02 Identities = 41/156 (26%), Positives = 55/156 (35%) Query: 710 TIAVPPTAQQPPPTIPTMIAAASPPSQPAVALSTIPGAVPITPPITTIAAAPPPSVTVGG 769 T + P T PPPT T + + PS P +T P +PPITT P P+ T Sbjct: 1398 TPSPPTTTPSPPPTTTTTLPPTTTPSPPTTTTTTPPPTTTPSPPITT-TTTPLPTTTPSP 1456 Query: 770 SLSSVLGPPVPEIKVKEEVEPMDIMRPVSAVPPLATNTVSPSLALLANNLSMPTSDLPPG 829 +S+ PP P P + P T T SP T+ PP Sbjct: 1457 PISTTTTPP PTTTPSPPTTTPSPPTTTPSPPTTTTTTP-PPTTTPSPPM 1504 Query: 830 ASPRKKPRKQQHVISTEEGDMMETNΞTDDEKSTAKS 865 +P P + T T +T +T S Sbjct: 1505 TTPITPPASTTTLPPTTTPSPPTTTTTTPPPTTTPS 1540 Score = 61 (9.2 bits), Expect = 1.6e-09, P = 1.6e-09 Identities = 23/93 (24%), Positives = 41/93 (44%) Query: 397 SVSHTQAPTSTIVTMTVPSHSSHATAVTTSNIPVAKVV PQQITHTSPRIQPDYPAE 452 S++ + +T T+T+P+ + T TT+ P + V P+ S I D+P+ Sbjct: 1257 SITTRPSTLTTFTTITLPTTPTSFTTTTTTTTPTSSTVLSTTPKLCCLWSDWINEDHPSS 1316 Query: 453 RSΞ LIPISGHRASPNPVAMETRSDNRPSVPVQ 484 S P G +P + E RS P + ++ Sbjct: 1317 GSDDGDREPFDGVCGAPEDI—ECRSVKDPHLSLE 1349 Score = 50 (7.5 bits), Expect = 8.0e-09, P = 8.0e-09 Identities = 16/41 (39%), Positives = 19/41 (46%) Query: 334 RPAQSRDVTTRITLPSHPALGTPKQQLHTMAQKTIFSTGTP 374 RP+ TT ITLP+ P T T T+ ST TP Sbjct: 1261 RPSTLTTFTT-ITLPTTPTSFTTTTTTTTPTSSTVLST-TP 1299 Score = 46 (6.9 bits), Expect = 5.4e-08, P = 5.4e-08 Identities = 24/106 (22%), Positives = 37/106 (34%) Query: 324 HPPSAAISIQRPAQSRDVTTRITLPSHPALGTPKQQLHTMAQKTIFSTGTPVAAATVAPI 383 +PP A++ + +S T + P G Q A G I Sbjct 1196 YPPGASVPTEETCKSCVCTNSSQVVCRPEEGKILNQTQDGAFCYWEICGPNGTVEKHFNI 1255 Query 384 LATNTIPSA-TTAGSVSHTQAPTSTIVTMTVPSHSSHATAVTTSNI 428 + T PS TT +++ PTS T T + +S TT + Sbjct 1256 CSITTRPSTLTTFTTITLPTTPTSFTTTTTTTTPTSSTVLSTTPKL 1301 Score = 44 (6.6 bits), Expect = 8.7e-08, P = 8.7e-08 Identities = 14/34 (41%), Positives = 17/34 (50%) Query: 478 RPSVPVQFQYF-LPTYPPΞAYPLAAHTYTPITSSV 511 RPS F LPT P S + T TP +S+V Sbjct: 1261 RPSTLTTFTTITLPTTPTS-FTTTTTTTTPTSSTV 1294 Pedant information for DKFZphtes3_2all, frame 2 Report for DKFZphtes3_2all .2 [LENGTH] 1048 [MW] 110324.04 [pi] 9.83 [HOMOL] PIR: 147141 gastric mucin (clone PGM-2A) - pig (fragment) 8e-15 [FUNCAT] 30.90 extracellular/secretion proteins [S. cerevisiae, YIR019c] le-09 [FUNCAT] 30.01 organization of cell wall [S. cerevisiae, YIR019c] le-09 [FUNCAT] 01.05.01 carbohydrate utilization [S. cerevisiae, YIR019c] le-09 [FUNCAT] 30.02 organization of plasma membrane [S. cerevisiae, YDR420w] 4e-09 [FUNCAT] 01.05.04 regulation of carbohydrate utilization [S. cerevisiae, YDR420w] 4e-09 [FUNCAT] 98 classification not yet clear-cut [S. cerevisiae, YJR151c] 4e-06 [FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YGR014w] le-05 [FUNCAT] 11.01 stress response [S. cerevisiae, YHL028w] le-04 [FUNCAT] 09.01 biogenesis of cell wall [S. cerevisiae, YHL028w] le-04 [EC] 3.2.1.3 Glucan 1, 4-alpha-glucosιdase 3e-08 [PIRKW] glycosidase 3e-08 [PIRKW] transmembrane protein 3e-08 [PIRKW] polysaccharide degradation 3e-08 [PIRKW] glycoprotein 9e-08 [PIRKW] calcium binding 9e-08 [PIRKW] hydrolase 3e-08 [PIRKW] cytoskeleton 7e-08 [SUPFAM] equine herpesvirus glycoprotein X 2e-07 [SUPFAM] yeast glucan 1, 4-alpha-glucosιdase homolog 3e-08 [SUPFAM] polymorphic epithelial mucin 7e-08 [SUPFAM] glucan 1, 4-alpha-glucosιdase homology 3e-08 [SUPFAM] equine herpesvirus 1 glycoprotein homology 2e-07 [PROSITE] MYRISTYL 9 [PROSITE] AMIDATION 1 [PROSITE] CAMP_PHOSPHO_SITE 2 [PROSITE] CK2_PHOSPHO_SITE 10 [PROSITE] PKC_PHOSPHO_SITE 12 [PROSITE] ASN_GLYCOSYLATION 3 [KW] Irregular [KW] LOW COMPLEXITY 20.04 % SEQ MGPPRHPQAGEIEAGGAGGGRRLQVEMSSQQFPRLGAPSTGLSQAPSQIANΞGSAGLINP SEG xxxxxxxxxxxx PRD ccccccccccccccccccccceeeeeeccccccccccccccccccccccccccccccccc SEQ AATVNDESGRDSEVSAREHMSSSSSLQSREEKQEPVVVRPYPQVQMLSTHHAVASATPVA SEG xxxxx xxxxxxxxxxxx PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ VTAPPAHLTPAVPLSFSEGLMKPPPKPTMPSRPIAPAPPSTLSLPPKVPGQVTVTMESSI SEG xxxxxxxxxxxxx xxxxxxxxx..xxxxxxxxxxxxxx PRD cccccccccccccccccccccccccccccccccccccccccccccccccccceeeccccc SEQ PQAΞAIPVATISGQQGHPΞNLHHIMTTNVQMSIIRΞNAPGPPLHIGASHLPRGAAAAAVM SEG xxxxx .. PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ SSSKVTTVLRPTSQLPNAATAQPAVQHIIHQPIQSRPPVTTΞNAIPPAVVATVSATRAQS SEG PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ PVITTTAAHATDSALSRPTLSIQHPPSAAISIQRPAQSRDVTTRITLPSHPALGTPKQQL SEG PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ HTMAQKTIFSTGTPVAAATVAPILATNTIPSATTAGSVSHTQAPTSTIVTMTVPSHSSHA SEG xxxxxxxxxx xxxxxx PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ TAVTTSNIPVAKVVPQQITHTSPRIQPDYPAERSSLIPISGHRASPNPVAMETRSDNRPS SEG xxxxxx PRD cccccccccccccccccccccccccccccccccccccccccccccccceeeecccccccc SEQ VPVQFQYFLPTYPPSAYPLAAHTYTPITSSVSTIRQYPVSAQAPNSAITAQTGVGVASTV SEG PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ HLNPMQLMTVDASHARHIQGIQPAPISTQGIQPAPIGTPGIQPAPLGTQGIHSATPINTQ SEG PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ GLQPAPMGTQQPQPEGKTSAVVLADGATIVANPISNPFSAAPAATTVVQTHSQSASTNAP SEG PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ AQGSSPRPSILRKKPATDGAKPKSEIHVSMATPVTVSMETVSNQNNDQPTIAVPPTAQQP SEG xxxxxxxxxxxxx PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ PPTIPTMIAAASPPSQPAVALSTIPGAVPITPPITTIAAAPPPSVTVGGSLSSVLGPPVP SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD cccccceeeccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ EIKVKEEVEPMDIMRPVSAVPPLATNTVSPSLALLANNLSMPTSDLPPGASPRKKPRKQQ SEG xxxxxxxxxx xxxxxxxxxx PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ HVISTEEGDMMETNSTDDEKSTAKSLLVKAEKRKSPPKEYIDEEGVRYVPVRPRPPITLL SEG xxxxxxxxxx .... PRD ccccccccccccccccccccchhhhhhhhhccccccccccccccccccccccccccccee SEQ RHYRNPWKAAYHHFQRYSDVRVKEEKKAMLQEIANQKGVSCRAQGWKVHLCAAQLLQLTN SEG PRD eeccccchhhhhhhccccchhhhhhhhhhhhhhhhhccceeecccceeehhhhhhhhhhc SEQ LEHDVYERLTNLQEGIIPKKKAATDDDLHRINELIQGNMQRCKLVMDQISEARDSMLKVL SEG PRD cchhhhhhhhhhhceeeeccccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh SEQ DHKDRVLKLLNKNGTVKKVSKLKRKEKV SEG xxxxxxxxxxxxx PRD hhhhhhhhhhccccceeeeeeeeccccc Prosite for DKFZphtes3_2all .2 PS00001 818->822 ASN_GLYCOSYLATION PDOC00001 PS00001 854->858 ASN_GLYCOSYLATION PDOC00001 PS00001 1033->1037 ASN_GLYCOSYLATION PDOC00001 PS00004 872->876 CAMP_PHOSPHO_SITE PDOC00004 PS00004 1037->1041 CAMP_PHOSPHO_SITE PDOC00004 PS00005 68->71 PKC_PHOSPHO_SITE PDOC00005 PS00005 75->78 PKC_PHOΞPHO_SITE PDOC00005 PS00005 242->245 PKC_PHOSPHO_SITE PDOC00005 PS00005 342->345 PKC_PHOSPHO_SITE PDOC00005 PS00005 355->358 PKC_PHOSPHO_SITE PDOC00005 PS00005 442->445 PKC_PHOSPHO_SITE PDOC00005 PS00005 513->516 PKC_PHOSPHO_SITE PDOC00005 PS00005 665->668 PKC_PHOSPHO_SITE PDOC00005 PS00005 831->834 PKC_PHOSPHO_SITE PDOC00005 PS00005 862->865 PKC_PHOSPHO_SITE PDOC00005 PS00005 940->943 PKC_PHOSPHO_SITE PDOC00005 PS00005 1035->1038 PKC_PHOSPHO_SITE PDOC00005 PS00006 63->67 CK2_PHOSPHO_SITE PDOC00006 PS00006 68->72 CK2_PHOSPHO_SITE PDOC00006 PS00006 75->79 CK2_PHOSPHO_SITE PDOC00006 PS00006 88->92 CK2_PHOΞPHO_SITE PDOC00006 PΞ00006 135->139 CK2_PHOSPHO_SITE PDOC00006 PS00006 473->477 CK2_PHOSPHO_SITE PDOC00006 PS00006 844->848 CK2_PHOSPHO_SITE PDOC00006 PS00006 855->859 CK2_PHOSPHO_SITE PDOC00006 PS00006 959->963 CK2_PHOSPHO_SITE PDOC00006 PS00006 984->988 CK2_PHOSPHO_SITE PDOC00006 PS00008 15->21 MYRISTYL PDOC00008 PS00008 16->22 MYRISTYL PDOC00008 PS00008 36->42 MYRISTYL PDOC00008 PS00008 233->239 MYRISTYL PDOC00008 PS00008 372->378 MYRISTYL PDOC00008 PS00008 533->539 MYRISTYL PDOC00008 PS00008 535->541 MYRISTYL PDOC00008 PS00008 590->596 MYRISTYL PDOC00008 PS00008 768->774 MYRISTYL PDOC00008 PS00009 19->23 AMIDATION PDOC00009 (No Pfam data available for DKFZphtes3_2all .2) DKFZphtes3_2al7 group: metabolism DKFZphtes3_2al7 encodes a novel 574 amino acid protein without similarity to known proteins. The novel protein contains a thiol protease cys pattern. Eukaryotic thiol proteases (EC 3.4.22.-) are a family of proteolytic enzymes containing an active site cysteine. Cathepsins belong to this protease family. The new protein can find application m modulation of proteolytic processes and as a new enzyme for proteomic analysis and biotechnologic production processes. unknown complete cDNA, complete eds, EST hits Sequenced by EMBL Locus: unknown Insert length: 2312 bp Poly A stretch at pos. 2300, polyadenylation signal at pos. 2273 1 GTTTTCACCT GATCATTAGA AACTAATGAA ACACCTTTTA AGTCTTATGA 51 ATTCAGGTTA CACTGTTTTC CAGATGCCTT GGCAGCTGGT ACAGGGCCTC 101 TGAAAAATGG AACCAAATTC TCTGAGGACT AAAGTCCCAG CTTTCTTATC 151 TGATTTGGGG AAGGCCACAT TGAGGGGAAT CAGAAAGTGT CCCCGATGTG 201 GCACATACAA TGGAACCCGG GGACTGAGCT GTAAGAACAA GACATGTGGA 251 ACCATATTCC GCTACGGTGC ACGCAAGCAG CCTAGTGTTG AAGCTGTCAA 301 AATCATTACA GGCTCTGATC TTCAGGTCTA CTCAGTGCGG CAAAGAGACC 351 GGGGCCCTGA TTACCGATGC TTTGTGGAGC TCGGGGTTTC AGAGACAACA 401 ATCCAGACAG TGGATGGGAC GATCATCACT CAGCTGAGCT CTGGACGGTG 451 TTATGTCCCC TCATGCCTGA AAGCTGCCAC TCAAGGCGTT GTGGAAAACC 501 AGTGCCAGCA CATCAAGCTG GCGGTGAACT GCCAGGCAGA GGCCACCCCT 551 CTGACCCTGA AGAGCTCGGT CCTGAATGCA ATGCAGGCCT CCCCGGAAAC 601 CAAACAGACC ATCTGGCAGT TGGCCACGGA ACCCACAGGT CCTCTGGTGC 651 AGAGAATTAC TAAAAACATC TTGGTGGTGA AATGCAAGGC AAGCCAGAAG 701 CACAGTTTGG GGTATTTGCA TACATCTTTT GTGCAGAAAG TCAGTGGCAA 751 AAGCTTGCCT GAGCGCCGCT TCTTCTGCTC CTGTCAGACT CTGAAATCGC 801 ACAAGTCAAA TGCCTCCAAG GATGAGACAG CCCAGAGATG CATTCATTTC 851 TTTGCTTGCA TCTGTGCCTT TGCCAGTGAT GAGACACTGG CTCAGGAATT 901 CTCAGACTTC CTAAATTTTG ATTCCAGCGG TCTTAAAGAG ATTATTGTAC 951 CCCAGTTAGG TTGCCATTCA GAATCAACAG TATCTGCTTG TGAGTCTACT 1001 GCCTCTAAGT CAAAGAAGAG GAGAAAGGAT GAAGTATCTG GTGCACAGAT 1051 GAACAGTTCA CTACTGCCTC AAGATGCAGT GAGCAGTAAT CTAAGGAAAA 1101 GTGGCCTGAA AAAGCCTGTG GTTGCTTCCT CGTTAAAAAG GCAGGCCTGT 1151 GGTCAGCTGT TAGATGAGGC ACAAGTGACT TTATCCTTCC AAGACTGGCT 1201 GGCCAGTGTC ACAGAACGCA TCCATCAAAC CATGCACTAT CAGTTTGATG 1251 GCAAACCAGA ACCATTGGTG TTCCACATTC CTCAGTCATT TTTTGATGCC 1301 CTGCAACAAA GAATATCTAT AGGAAGTGCA AAAAAACGGC TCCCCAACTC 1351 CACCACAGCT TTTGTTCGGA AAGATGCCTT GCCACTGGGA ACCTTTTCCA 1401 AGTATACTTG GCATATCACT AATATCCTGC AAGTTAAACA AATCTTAGAT 1451 ACCCCAGAGA TGCCCTTGGA AATCACCCGT AGCTTTATCC AGAACCGAGA 1501 TGGGACTTAT GAGCTATTTA AATGCCCTAA AGTGGAAGTA GAAAGCATAG 1551 CAGAAACCTA CGGTCGTATA GAAAAACAAC CAGTGCTGCG ACCCTTGGAA 1601 CTAAAAACTT TTCTCAAAGT TGGCAACACT TCCCCAGATC AAAAGGAGCC 1651 AACACCTTTC ATCATCGAGT GGATCCCAGA TATCCTTCCC CAATCTAAGA 1701 TTGGCGAGCT GCGGATCAAG TTTGAGTATG GCCACCACCG GAATGGGCAT 1751 GTGGCGGAGT ACCAAGACCA GCGGCCCCCC TTGGACCAGC CCTTGGAACT 1801 GGCCCCTCTG ACCACTATTA CTTTCCCTTA AAGCAAAACA AGATAATAAT 1851 CTTTTGCTGC TTAATTTGCA CATCCCCACC CCTTGACAAC TTTAAATGCT 1901 AGTTAGGCAC TTAGATGGCC CTGTTCCTTG GTAAACTGCT CTTAGCTAAG 1951 ATGCAAATTC TCAGTGCTTT CAAGTGGATT CTGTTGAAGA AAATCTCTTG 2001 TAAATAGCCT TTTTGATGCT GCTGTGTACA GTCTTCATTA TGCATTGGGC 2051 AGTATTTCTG GCTAGAGTTT TAAAAGGAAC AGAAAGAAAA CCAGCTTATT 2101 TTCCTTCTTA CGGACTCATC TTTAGCGTTT ATTTCAACCT TTTGCTAATT 2151 CTCTGAGAAA TCTGCAGCAC TCAGCCATAC ACCAACAGTG TTGGAAAGTT 2201 AACACCCTGG TTAGGGCAGA ATGTTAAAGA CCATCTTGGC AGAGTTCCAG 2251 CCACGCTCTT TATTCTGTTC TCAAATAAAG CAGTGTCACT AGTTTTTCCT 2301 AAAAAAAAAA AA BLAST Results o BLAST result Medlme entries No Medlme entry Peptide information for frame 2 ORF from 107 bp to 1828 bp; peptide length: 574 Category: putative protein 1 MEPNSLRTKV PAFLSDLGKA TLRGIRKCPR CGTYNGTRGL SCKNKTCGTI 51 FRYGARKQPS VEAVKIITGS DLQVYSVRQR DRGPDYRCFV ELGVSETTIQ 101 TVDGTIITQL SSGRCYVPSC LKAATQGVVE NQCQHIKLAV NCQAEATPLT 151 LKSSVLNAMQ ASPETKQTIW QLATEPTGPL VQRITKNILV VKCKASQKHS 201 LGYLHTSFVQ KVSGKSLPER RFFCSCQTLK SHKSNASKDE TAQRCIHFFA 251 CICAFASDET LAQEFSDFLN FDSSGLKEII VPQLGCHSEΞ TVSACESTAS 301 KSKKRRKDEV SGAQMNSSLL PQDAVSSNLR KSGLKKPVVA SSLKRQACGQ 351 LLDEAQVTLS FQDWLASVTE RIHQTMHYQF DGKPEPLVFH IPQSFFDALQ 401 QRISIGSAKK RLPNSTTAFV RKDALPLGTF SKYTWHITNI LQVKQILDTP 451 EMPLEITRSF IQNRDGTYEL FKCPKVEVES IAETYGRIEK QPVLRPLELK 501 TFLKVGNTSP DQKEPTPFII EWIPDILPQS KIGELRIKFE YGHHRNGHVA 551 EYQDQRPPLD QPLELAPLTT ITFP BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_2al7, frame 2 No Alert BLASTP hits found Pedant information for DKFZphtes3_2al7, frame 2 Report for DKFZphtes3_2al7.2 [LENGTH] 574 [MW] 64076.89 [pi] 9.15 [PROSITE] MYRISTYL 5 [PROSITE] CK2_PHOSPHO_SITE 9 [PROSITE] PKC_PHOSPHO_SITE 14 [PROSITE] ASN_GLYCOSYLATION 5 [PROSITE] THIOL_PROTEASE_CYS 1 [KW] Alpha_Beta SEQ MEPNSLRTKVPAFLSDLGKATLRGIRKCPRCGTYNGTRGLSCKNKTCGTIFRYGARKQPS PRD ccccccccccchhhhhcccchhhhhcccccccccccccccccccccccceeeeccccccc SEQ VEAVKIITGSDLQVYSVRQRDRGPDYRCFVELGVSETTIQTVDGTIITQLSSGRCYVPSC PRD ceeeeeeecccceeeeeccccccccceeeeeecccccceeeccceeeeeecccccccchh SEQ LKAATQGVVENQCQHIKLAVNCQAEATPLTLKSSVLNAMQASPETKQTIWQLATEPTGPL PRD hhhhhhhhcchhhhheeehhhhhhhcccccchhhhhhhhhcccchhhhhhhhhcccccch SEQ VQRITKNILVVKCKASQKHSLGYLHTSFVQKVSGKSLPERRFFCSCQTLKSHKSNASKDE PRD hhhhhhheeeeeecccccccccccceeeeeeecccccccceeeecccccccccccccccc SEQ TAQRCIHFFACICAFASDETLAQEFSDFLNFDSSGLKEIIVPQLGCHSESTVSACESTAS PRD hhhhhhhhhhhhhhhhhchhhhhhhhhhhccccccceeeeeecccccccceeeccccccc SEQ KSKKRRKDEVSGAQMNSSLLPQDAVSSNLRKSGLKKPVVASSLKRQACGQLLDEAQVTLS PRD ccchhhhhccccccccccccccccchhhhhhhccccceeehhhhhhhhhchhhhhhhhhh SEQ FQDWLASVTERIHQTMHYQFDGKPEPLVFHIPQSFFDALQQRISIGSAKKRLPNSTTAFV PRD hhhhhhhhhhhhhhhhhhhcccccccceeehhhhhhhhhhhhhhhhcccccccccceeee SEQ RKDALPLGTFSKYTWHITNILQVKQILDTPEMPLEITRSFIQNRDGTYELFKCPKVEVES PRD ecccccccccceeeeehhhhhhhhhhhccccccccceeeeeeccccceeeecccceeeeh SEQ IAETYGRIEKQPVLRPLELKTFLKVGNTSPDQKEPTPFIIEWIPDILPQSKIGELRIKFE PRD hhhhhhhhhccccccccccceeeeecccccccccccceeeeecccccccccccceeeeee 1313 ' , 1313 < o o o IT 1 n o n n o 3" DKFZphtes3_2dl5 group: testes derived DKFZphtes3_2dl5 encodes a novel 274 amino acid protein with similarity to C. elegans cosmid F25H2.1. The novel protein contains a Pfam predicted C2-domaιn. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . similarity to C. elegans F25H2.1 complete cDNA, complete eds, EST hits Sequenced by EMBL Locus : unknown Insert length: 3615 bp Poly A stretch at pos. 3603, polyadenylation signal at pos. 3578 1 GCGGCGGCCT CGAGGTGACA ACTGTCTCCG TCGCAGGCTC CGGCGGGGGC 51 GCAGGAGGTC GCCCGGCGCG TCACTGTCGG GTCGGCGAGC CACGGGGGCC 101 GCCGCAGCAC CATGGCGACC ACCGTCAGCA CTCAGCGCGG GCCGGTGTAC 151 ATCGGTGAGC TCCCGCAGGA CTTCCTCCGC ATCACGCCCA CACAGCAGCA 201 GCGGCAGGTC CAGCTGGACG CCCAGGCGGC CCAGCAGCTG CAGTACGGAG 251 GCGCAGTGGG CACCGTGGGC CGACTGAACA TCACGGTGGT ACAGGCAAAG 301 TTGGCCAAGA ATTACGGCAT GACCCGCATG GACCCCTACT GCCGACTGCG 351 CCTGGGCTAC GCGGTGTACG AGACGCCCAC GGCACACAAT GGCGCCAAGA 401 ATCCCCGCTG GAATAAGGTC ATCCACTGCA CGGTGCCCCC AGGCGTGGAC 451 TCTTTCTATC TCGAGATCTT CGATGAGAGA GCCTTCTCCA TGGACGACCG 501 CATTGCCTGG ACCCACATCA CCATCCCGGA GTCCCTGAGG CAGGGCAAGG 551 TGGAGGACAA GTGGTACAGC CTGAGCGGGA GGCAGGGGGA CGACAAGGAG 601 GGCATGATCA ACCTCGTCAT GTCCTACGCG CTGCTTCCAG CTGCCATGGT 651 GATGCCACCC CAGCCCGTGG TCCTGATGCC AACAGTGTAC CAGCAGGGCG 701 TTGGCTATGT GCCCATCACA GGGATGCCCG CTGTCTGTAG CCCCGGCATG 751 GTGCCCGTGG CCCTGCCCCC GGCCGCCGTG AACGCCCAGC CCCGCTGTAG 801 CGAGGAGGAC CTGAAAGCCA TCCAGGACAT GTTCCCCAAC ATGGACCAGG 851 AGGTGATCCG CTCCGTGCTG GAAGCCCAGC GAGGGAACAA GGATGCCGCC 901 ATCAACTCCC TGCTGCAGAT GGGGGAGGAG CCATAGAGCC TCTGCCTCGA 951 TGCCGTTTTG CCCCCGCTCT TTGGACACGC CGACCCGGCG CTCCCCAAGG 1001 AATGCTGTCC CAACAAGATT CCCGTGAAAG AGCACCCGTG TCGCCCCCTC 1051 CCGTGGACTT CTGTGCCGCC CCGTCCACAC CTGTTCTTGG GTGCATGTGG 1101 GTTTTCGGTT CCTGGCGGTC CAGGACGGGG CGGGGGCTCC CCTCCCATCT 1151 CGTGCTGGGA GGTCTCAGCG CGCTCTCCTG TCCCTGGGAC GTGCGTCTCT 1201 CCTTCTCATG CCGTTCTGGA AAATGCTCTT GCTGTAGAGA GCAGCTGCTT 1251 CTGCCAGGGT GTTGGAGGTG GTGGAGCGCC TTCCGATTCC ATTCATGGCA 1301 TTTTGTGATG TGATGTAATT GGAATAGAGC TGTTGATTTA AGGCACACAC 1351 AATCCCTCAC ACTGTGGGTT TTTTTTAGAA CTTCCCAGAC GAAAACTCAC 1401 GCCCTTGCCC TAACGCGCTT TGCTGTGAGC CTGGCCCCTG CCCAGGGCTT 1451 GGGTCTGGTG AGCTGAGCAG CTTCCTGTGG ATGGTGTGGG GCCGGCCTCT 1501 GGCCTGGCTC ACCTGGCCAC TGTCCAGCCA GCCTTGTGAC AGACTCCGGC 1551 CTGAAGGCAG AATGAACCCA CACCTGGAGT GAGGAAGGGG GCCTGGCACG 1601 GTTGGCCAGG CTCTGCCTGA TTGCCAGCCA GCGGGCATCT GAAGCCGGGT 1651 CCTTCGCCCG CCGGAGGCTG CCGTCCGTCT CTCCTGCTGC GCTCGTGCCA 1701 GCTCCGTGGG TGTCCTCCCA GGGAGCTTCT CTTCTCAACA GGCCTTGCGA 1751 GGCTGGGGTG AGAGGTGATA GAGGCAGCAC TGTGCATGAT TCCGAGAGGG 1801 TGTGGTGGCA CTGCCAGCCG ACTGCTGACA GCTTGGGAGC TGCTGTGCCC 1851 AGGACGTGGG TTCAGCGTGG GCGAGGAAAG CCTGGCGAGC GTGGCCCTGT 1901 AAAAGCTTTC TGAGGCGGGA GGCGCTCACT TACCTCTGAC TGCCTGGGCG 1951 CTGCGTGTAG CATCTTGGCC TACAGGACAG ATTTTAGGTG ACACCTGGTT 2001 ATGACAGTCA GAAATTTGAG AAGCTTCTCA CAAGTGATGC ACTTTAAATA 2051 ATCTGCATGC CATTGAGACA CCTGCATGTC TGGTGTTTGT GGTTCAAGTG 2101 TCTTGCCGCC GGCCTTCGGA TGTAAACCCA CTGATAACGG ACAGAAAGAG 2151 AATGCCCACA AGTGGGTCTT CTGTGGAAGA TGCAGAAGGA GGAAGTTAGT 2201 GCTTACATTT TAGTCTTTTT CTCCCTCAAA AAAATAGGTT AAGTTTCAGT 2251 GCCAGCTAGA AAATACTGCT TTCTGCCATC GATTGGGGGT GGTTTTTGTC 2301 AAATATACTG TTGATAAATA TTTATTTTTG TAAACTTGAA GTGTGTGGTG 2351 GCCGTGGGGG AGGGACATGC TGGCAGCAGG CGCCTTCTTC AGCTGTGGGT 2401 CCTAAAGGCC TTTGATCCTT TGAAGAAGAA AGACATGGTA TTTGTTCAGC 2451 AGACGCCGAC CACTCAGACG GAGGGGCCCC TGGGATTCCC TGTCTCAGAT 2501 GGCCTGGTCT TACGCCTGTG TAGATTTCTT CTCCATTGGG AATGAAGGTG 2551 TCAGGCGGGA CTGGAACGTT CTAGATGGTA TGTTCCGTGA TATTAACAAC 2601 TCTAACCCAG GACAGACCAC AAGCCACACT CAGAGGCCTC ACTGTGCTGG 2651 GGGCTTCGGT GTCCAGGCGC CCAGGTGTGG CCACCAGCAC CGGTTTCTGC 2701 CTTCGCGTTG CTGGGGTGCA GTGAGACTGC CACACGCGTG CACATGTGGC 2751 TCTGTGGGTG TCTCCTAGAG AGGACGTGGC CCCTGCTGCC AGCCCTTGAG 2801 CAGCCCGTGT GGGGGCCCGA GGGACCCACA CAGTGGGGGC CAGCCTCGCT 2851 GGAGGGAGAG CAACCCTTTG CCGATGACCA CGCTTGCCGC CATCTCTTAG 2901 TTTTCTTTTT CACAAGCGCT TTATTTTTTT AATAGACAAA TCACATTTTG 2951 CAAGGCCTTT AATTAAATAA GATTCTTCTT TCCTTCATTT TATGCTTTAT 3001 TTCCTGTTTG AAGGCTTACT GTAGAAGTGG CTTACTGTAG AAGCAGCTTG 3051 CTGAGCCCCT CCGAGCGGTC CCCAGAATTA GCTGGTTCAC AACCCCCACC 3101 CTCCCCCGCC CCCGCCTGTG TCAGGTGTGG ATGAGGTCGT CACACTCAGA 3151 AGGACAGGCT TGTCTGCCAG CTCACAAGGG GAGGCTGCAG TGGGTTTGGG 3201 AGCTGGGTTT AGGCCCCTGG TGTCTGAGGG CCCAGGCCTT GCCAGCCTCT 3251 GCTGCTCCTG CTCCTGGGTT TGAAGATGCA GGCCGATCGC CAGCTCCGTG 3301 GCAGCGGTCA CTAAGGACAG CCTGACTGTG CCATCTTGGA GCCTCAGGCG 3351 GGGCTCCGGA GATAGAAGAC AGGTCGCCGG AGGCTCCCCC TCCTCTCCTC 3401 TCCCCTCTGC AGATGCTCCC TGGGCGCTAC CCTGCAGGGT GCCAGGCAGG 3451 AGTGGTCTCA GAACGTGCGC TTCTGATTAT TTTACTGGGG TCCATTGTCC 3501 AGATTTTTCT TTGATTGTAA AATATATTTT TACTTTTTAG TCTTCTAATT 3551 TAATAAATGA TCCATATAAA AATAGAGAAA TAAAGTCCTT TAAGGGAAGG 3601 TTTAAAAAAA AAAAA BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 1 ORF from 112 bp to 933 bp; peptide length: 274 Category: similarity to unknown protein Classification: no clue 1 MATTVSTQRG PVYIGELPQD FLRITPTQQQ RQVQLDAQAA QQLQYGGAVG 51 TVGRLNITVV QAKLAKNYGM TRMDPYCRLR LGYAVYETPT AHNGAKNPRW 101 NKVIHCTVPP GVDSFYLEIF DERAFSMDDR IAWTHITIPE SLRQGKVEDK 151 WYSLSGRQGD DKEGMINLVM SYALLPAAMV MPPQPVVLMP TVYQQGVGYV 201 PITGMPAVCS PGMVPVALPP AAVNAQPRCS EEDLKAIQDM FPNMDQEVIR 251 SVLEAQRGNK DAAINSLLQM GEEP BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_2dl5, frame 1 TREMBL :CEF25H2_1 gene: "F25H2.1"; Caenorhabditis elegans cosmid F25H2, N = 1, Score = 385, P = 1. le-35 >TREMBL:CEF25H2_1 gene: "F25H2.1"; Caenorhabditis elegans cosmid F25H2 Length = 457 HSPs: Score = 385 (57.8 bits), Expect = 1. le-35, P = 1. le-35 Identities = 77/182 (42%), Positives = 118/182 (64%) Query: 4 TVSTQRGPVYIGELPQDFLRIT-PTQQQRQVQLDAQAAQQLQYGGAVGTVGRLNITVVQA 62 TV+ +R V +GELP FLR+ P QQ + ++ Q + + + T GRL++T+++A Sbjct: 5 TVAERRRQVLVGELPPHFLRLAVPIQQTAEPEI-VQP-RMVSFVPP-NTRGRLSVTILEA 61 Query: 63 KLAKNYGMTRMDPYCRLRLGYAVYETPTAHNGAKNPRWNKVIHCTVPPGVDSFYLEIFDE 122 L KNYG+ RMDPYCR+R+G ++T A + P WN+ ++ +P V+S Y++IFDE Sbjct: 62 NLVKNYGLVRMDPYCRVRVGNVEFDTNVAANAGRAPTWNRTLNAYLPMNVESIYIQIFDE 121 Query: 123 RAFSMDDRIAWTHITIPESLRQGKVEDKWYSLSGRQGDDKEGMINLVMSYAL—LPAAMV 180 +AF D+ IAW HI +P ++ G D+++ LSG+QG+ KEGMI+L S+A LP Sbjct: 122 KAFGPDEVIAWAHIMLPLAIFNGDNIDEYFQLSGQQGEGKEGMIHLHFSFAPIDLPLQQA 181 Query: 181 MPPQP 185 P +P Sbjct: 182 APAEP 186 Score = 92 (13.8 bits), Expect = 1.8e-01, P = 1.7e-01 Identities = 26/68 (38%), Positives = 38/68 (55%) Query: 194 QQGVGYVPITGMPAVCSPGMVPV—ALP--PAAVNAQPRCSEEDLKAIQDMFPNMDQEVI 249 QQG G + + +P +P+ A P PA +EED K IQ+MFP +D+EVI Sbjct: 156 QQGEGKEGMIHLHFSFAPIDLPLQQAAPAEPAPAPLPVEITEEDTKEIQEMFPIVDKEVI 215 Query: 250 RSVLEAQR 257 + +LE +R Sbjct: 216 KCILEERR 223 Pedant information for DKFZphtes3_2dl5, frame 1 Report for DKFZphtes3_2dl5.1 [LENGTH] 274 [MW] 30281.97 [pi] 5.68 [HOMOL] TREMBL :CEF25H2_1 gene: "F25H2.1"; Caenorhabditis elegans cosmid F25H2 4e-36 [PFAM] C2 domain [KW] Alpha_Beta [KW] LOW COMPLEXITY 16.42 % SEQ MATTVSTQRGPVYIGELPQDFLRITPTQQQRQVQLDAQAAQQLQYGGAVGTVGRLNITVV SEG xxxxxxxxxxxxxxxxx PRD cccccccccceeeeeccccceeeecccchhhhhhhhhhhhhhhhhcccccceeeeceeeh SEQ QAKLAKNYGMTRMDPYCRLRLGYAVYETPTAHNGAKNPRWNKVIHCTVPPGVDSFYLEIF SEG PRD hhhhhhhhcccccccchhhhheeeeeecccccccccccccceeeeeccccccceeeeeec SEQ DERAFSMDDRIAWTHITIPESLRQGKVEDKWYSLSGRQGDDKEGMINLVMSYALLPAAMV SEG xxxxxxxx PRD cccccccccceeeeccccccccccccccceeeeeccccccccccceeeeehhhhhhhhhc SEQ MPPQPVVLMPTVYQQGVGYVPITGMPAVCSPGMVPVALPPAAVNAQPRCSEEDLKAIQDM SEG xxxxxxxxxx xxxxxxxxxx PRD ccccceeeeeeeeecccccccccccceeecccccccccccceeeeccccchhhhhhhhhc SEQ FPNMDQEVIRSVLEAQRGNKDAAINSLLQMGEEP SEG PRD ccccchhhhhhhhhhhccccchhhhhhhhhhccc (No Prosite data available for DKFZphtes3_2dl5.1) Pfam for DKFZphtes3_2dl5.1 HMM_NAME C2 domain HMM *LtVrIIeARNLWkMDMnGfSDPYVKVdMdPdpkDtkKWKTkTιWNNGLN L++++++A+ + + M+ DPY+++ + + + +T T +N N Query 55 LNITVVQAKLAKNYGMT-RMDPYCRLRLGYAVY ETPTAHNGAKN 97 HMM PVWNEEeFvFedlPyPdlqrkMLRFaVWDWDRFSRBDFIGHCi* P+WN + +P + + ++++D+ FS +D 1+ + Query 98 PRWN-KVIHCT-VPPGVDSF YLEIFDERAFSMDDRIAWTH 135 DKFZphtes3_2el2 group: Transcription Factors DKFZphtes3_2el2 encodes a novel 849 ammo acid protein with similarity to Zmc finger proteins . The new protein is a putative transcription factor with three C2H2 zmc fingers. Additionally, a cytochrome C family heme-binding site signature is present m the protein, which is only found in cytochrom C related proteins. The new protein can find application m modulating/blocking the expression of genes controlled by this transcription factor. similarity to fmger proteins complete cDNA, complete eds, 5 EST hits Sequenced by EMBL Locus: unknown Insert length: 3205 bp Poly A stretch at pos. 3192, polyadenylation signal at pos. 3171 1 GGCACGGCCG GGTCCTGGCT GGCCAAACGA GGCTCGCGGA AGCAGCAGCC 51 GCCGCCTGAC CGCAGCTGGA TTTTGAAGAT TGATCCAAGG GACTGTATTA 101 ATTTCAGGAA TTGATTTGAA AGACACTGGC TCTGCCACTT AACAGCCATG 151 TAACCTTGGA TATGGAAGAA AGTAGCAGTG TTGCCATGTT GGTGCCAGAT 201 ATTGGGGAAC AGGAAGCTAT ACTGACTGCT GAAAGTATCA TCAGTCCTTC 251 ATTGGAAATT GATGAACAAA GAAAAACTAA ACCAGATCCA TTAATCCATG 301 TTATCCAGAA GTTAAGCAAG ATAGAAAAAT GAAAAGTCAC AAAAATGTCT 351 TTTAATTGGG AAGAAACGCC CACGTTCAAG TGCTGCAACA CACTCTCTTG 401 AAACCCAAGA ACTTTGTGAG ATTCCGGCTA AAGTAATCCA GTCACCTGCT 451 GCTGATACTA GAAGGGCTGA GATGTCACAA ACAAATTTTA CCCCTGACAC 501 TCTTGCCCAG AATGAAGGGA AGGCTATGTC TTATCAGTGT AGCCTTTGTA 551 AGTTTCTATC ATCATCCTTT TCCGTGTTAA AAGATCATAT TAAGCAACAT 601 GGTCAGCAAA ATGAAGTGAT ACTGATGTGC TCAGAGTGCC ATATTACATC 651 TAGAAGCCAG GAGGAACTTG AAGCCCACGT GGTGAATGAC CATGACAATG 701 ATGCCAATAT CCACACCCAA TCCAAAGCCC AACAGTGCGT AAGCCCCTCC 751 AGCTCTTTGT GTCGGAAAAC CACAGAAAGA AATGAAACCA TTCCAGATAT 801 CCCAGTAAGT GTGGACAATC TACAGACTCA TACTGTCCAA ACTGCATCTG 851 TGGCAGAAAT GGGTAGGAGG AAATGGTATG CATACGAACA GTACGGCATG 901 TATCGATGCT TGTTTTGTAG TTATACTTGT GGCCAGCAGA GAATGTTGAA 951 AACACACGCT TGGAAACATG CTGGGGAGGT TGATTGCTCC TATCCAATCT 1001 TTGAAAATGA AAATGAACCC CTAGGCCTGC TGGATTCTTC AGCAGCTGCT 1051 GCGCCTGGTG GGGTCGATGC AGTCGTCATT GCTATTGGAG AGAGTGAACT 1101 GAGTATCCAC AATGGGCCAT CAGTGCAAGT GCAGATTTGC AGCTCAGAAC 1151 AGTTATCATC TTCATCTCCT TTAGAACAGA GTGCAGAAAG AGGAGTACAC 1201 CTAAGTCAGT CAGTTACCCT GGACCCCAAT GAGGAAGAAA TGCTAGAAGT 1251 GATTTCTGAT GCAGAGGAGA ATCTGATTCC TGATAGCCTG CTTACATCAG 1301 CACAGAAAAT CATCAGCAGC AGCCCCAATA AAAAAGGGCA TGTTAACGTG 1351 ATAGTGGAGC GATTGCCAAG TGCTGAAGAA ACCCTTTCAC AGAAGCGCTT 1401 CCTCATGAAC ACTGAAATGG AAGAAGGGAA GGACCTGAGC CTGACAGAAG 1451 CTCAGATTGG GCGCGAAGGA ATGGATGATG TTTATCGTGC TGATAAATGT 1501 ACTGTTGATA TTGGGGGATT GATCATAGGC TGGAGCAGTT CAGAGAAAAA 1551 AGACGAGTTA ATGAATAAAG GCCTGGCTAC TGATGAGAAT GCCCCACCAG 1601 GCCGGAGAAG GACAAATTCT GAGTCTCTTC GATTACACTC ATTAGCTGCA 1651 GAAGCCCTTG TCACAATGCC TATAAGAGCT GCAGAGTTGA CAAGAGCCAA 1701 CCTGGGGCAC TATGGAGATA TAAACCTTTT AGATCCAGAT ACTAGTCAAA 1751 GGCAAGTAGA TAGTACATTG GCAGCGTACT CAAAAATGAT GTCGCCACTT 1801 AAAAACTCTT CAGATGGATT AACTAGTCTT AACCAAAGCA ACTCCACCTT 1851 GGTAGCACTC CCAGAGGGTA GGCAGGAATT GTCAGATGGG CAGGTTAAGA 1901 CAGGCATCAG CATGTCCTTA CTCACCGTCA TTGAAAAATT GAGAGAAAGG 1951 ACAGACCAAA ACGCTTCAGA CGATGACATT TTGAAAGAGT TGCAGGACAA 2001 CGCCCAGTGC CAACCCAACA GCGATACAAG TTTGTCCGGA AACAATGTGG 2051 TGGAATACAT CCCGAATGCT GAACGACCCT ACCGTTGCCG CCTGTGTCAC 2101 TACACAAGTG GCAACAAGGG CTACATCAAG CAGCACTTAC GAGTCCATCG 2151 ACAGAGACAG CCTTATCAGT GTCCTATCTG CGAGCACATA GCGGACAACA 2201 GCAAAGATTT GGAGAGTCAC ATGATCCACC ACTGTAAGAC AAGAATATAC 2251 CAGTGCAAGC AGTGTGAAGA ATCCTTCCAT TATAAGAGTC AATTGAGGAA 2301 CCATGAGAGA GAACAGCACA GTCTTCCAGA TACCTTGTCA ATAGCAACTT 2351 CTAATGAGCC AAGAATTTCC AGTGATACAG CTGATGGAAA ATGTGTCCAG 2401 GAAGGGAATA AGTCTTCAGT CCAGAAACAA TATAGATGTG ATGTGTGTGA 2451 TTATACAAGT ACAACATATG TTGGTGTCAG AAACCACAGG CGAATCCATA 2501 ACTCTGATAA GCCGTACAGA TGCTCTCTGT GTGGGTATGT GTGTAGCCAT 2551 CCTCCTTCTT TGAAGTCTCA TATGTGGAAA CATGCAAGTG ACCAAAATTA 2601 CAACTACGAA CAAGTAAACA AGGCTATTAA CGACGCGATT TCACAAAGTG 2651 GCAGAGTTCT GGGGAAATCC CCTGGAAAGA CTCAATTAAA GAGCAGTGAA 2701 GAGAGTGCAG ATCCCGTCAC TGGAAGTTCG GAAAATGCAG TGTCATCTTC 2751 AGAACTGATG TCCCAGACTC CCAGTGAAGT TCTGGGTACC AACGAGAATG 2801 AGAAACTGAG CCCTACAAGT AATACCTCAT ATAGTTTAGA AAAAATCTCC 2851 AGTCTGGCCC CTCCTAGCAT GGAGTACTGC GTTTTACTCT TCTGCTGTTG 2901 TATTTGTGGT TTTGAATCAA CCAGCAAAGA AAACCTCTTG GATCATATGA 2951 AAGAGCACGA GGGTGAAATT GTAAACATCA TCCTGAATAA GGACCACAAT 3001 ACAGCTCTAA ACACAAATTA GGTGGAATAA TGACTCGAGC AGGAAAGCAG 3051 TAGAAGAGGA TTCCTTCACC ACAGTTTCAC CTTTACGCTG TCAGACAACT 3101 TCCTGCCACA GAAGAAGTCG TTGATGTGAT TTTTGAGGAA ATGACAGATG 3151 TGACTTTGGA ACCAAACTTG TAATAAAAGG AATTCCAAAT GGAAAAAAAA 3201 AAAAA BLAST Results No BLAST result Medlme entries 90301500: Cloning and sequencing of a zmc finger cDNA expressed m mouse testis. 92310982: Zfp-37, a new murine zinc finger encoding gene, is expressed in a developmentally regulated pattern in the male germ line. Peptide information for frame 1 ORF from 472 bp to 3018 bp; peptide length: 849 Category: similarity to known protein 1 MSQTNFTPDT LAQNEGKAMS YQCSLCKFLS SSFSVLKDHI KQHGQQNEVI 51 LMCSECHITS RSQEELEAHV VNDHDNDANI HTQSKAQQCV SPSSSLCRKT 101 TERNETIPDI PVSVDNLQTH TVQTASVAEM GRRKWYAYEQ YGMYRCLFCS 151 YTCGQQRMLK THAWKHAGEV DCSYPIFENE NEPLGLLDSS AAAAPGGVDA 201 VVIAIGESEL SIHNGPSVQV QICSSEQLSS SSPLEQSAER GVHLSQSVTL 251 DPNEEEMLEV ISDAEENLIP DSLLTSAQKI ISSSPNKKGH VNVIVERLPS 301 AEETLSQKRF LMNTEMEEGK DLSLTEAQIG REGMDDVYRA DKCTVDIGGL 351 IIGWSSSEKK DELMNKGLAT DENAPPGRRR TNSESLRLHS LAAEALVTMP 401 IRAAELTRAN LGHYGDINLL DPDTSQRQVD STLAAYSKMM SPLKNSSDGL 451 TSLNQSNSTL VALPEGRQEL SDGQVKTGIS MSLLTVIEKL RERTDQNASD 501 DDILKELQDN AQCQPNSDTS LSGNNVVEYI PNAERPYRCR LCHYTSGNKG 551 YIKQHLRVHR QRQPYQCPIC EHIADNSKDL ESHMIHHCKT RIYQCKQCEE 601 SFHYKSQLRN HEREQHSLPD TLSIATΞNEP RISSDTADGK CVQEGNKSSV 651 QKQYRCDVCD YTSTTYVGVR NHRRIHNSDK PYRCSLCGYV CSHPPSLKSH 701 MWKHASDQNY NYEQVNKAIN DAISQSGRVL GKSPGKTQLK SSEESADPVT 751 GSSENAVSSS ELMSQTPSEV LGTNENEKLS PTSNTSYSLE KISSLAPPSM 801 EYCVLLFCCC ICGFESTSKE NLLDHMKEHE GEIVNIILNK DHNTALNTN BLASTP hits Entry S10245 from database PIR: finger protein, testis - mouse Score = 265, P = 8.4e-23, identities = 61/205, positives = 91/205 Entry S22954 from database PIR: fmger protein zfp-37 - mouse Score = 265, P = 9.1e-22, identities = 61/205, positives = 91/205 Entry AF031657_1 from database TREMBL: gene: "Zfp94"; product: "zmc-fmger protein 94"; Rattus norvegicus zinc-finger protein 94 (Zfp94) gene, partial eds. Score = 243, P = 1.6e-21, identities = 57/190, positives = 85/190 Alert BLASTP hits for DKFZphtes3_2el2, frame 1 No Alert BLASTP hits found Pedant information for DKFZphtes3_2el2, frame 1 Report for DKFZphtes3_2el2.1 [LENGTH] 849 [MW] 94325. 42 [pi] 5.47 [HOMOL] PIR:A5 4661 zmc finger protein ZNF41 - human (fragment) 2e-22 [FUNCAT) 04.05. 01.04 transcriptional control [Ξ. cerevisiae, YJL056c] 3e-09 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YJL056c] 3e-09 [FUNCAT] 04.03. 01 trna synthesis [Ξ. cerevisiae, YPR186c PZF1 - TFIIIA] le-07 [FUNCAT] 04.01. 01 rrna synthesis [S. cerevisiae, YPR186c PZF1 - TFIIIA] le-07 [FUNCAT] 04.99 other transcription activities [S. cerevisiae, YOR113w] 4e-07 [FUNCAT] 01.05. 04 regulation of carbohydrate utilization [S. cerevisiae, YGL209w] 2e-04 [FUNCAT] 13.04 homeostasis of other ions [S. cerevisiae, YNL027w] 2e-04 [FUNCAT] 11.01 stress response [S. cerevisiae, YMR037c] 3e-04 [BLOCKS] BL00028 Zinc finger, C2H2 type, domain proteins [SCOP] dlmeyg_ 9.6.1.1.1 a designed zinc fmger protein [syntheti 8e-06 [PIRKW] nucleus 8e-18 [PIRKW] RNA binding 5e-13 [PIRKW] duplication 7e-13 [PIRKW] tandem repeat le-21 [PIRKW] spermatogenesis 6e-16 [PIRKW] zinc 9e-21 [PIRKW] zinc finger le-21 [PIRKW] DNA binding le-21 [PIRKW] metal binding 3e-15 [PIRKW] phosphoprotein 5e-13 [PIRKW] leucine zipper le-13 [PIRKW] alternative splicing 6e-18 [PIRKW] eye lens 2e-16 [PIRKW] oocyte le-12 [PIRKW] transcription factor 6e-18 [PIRKW] segmentation 7e-13 [PIRKW] embryo le-12 [PIRKW] transcription regulation 2e-19 [PIRKW] homeobox 2e-08 [SUPFAM] POZ domain homology 7e-15 [SUPFAM] transcription factor Krueppel 7e-13 [SUPFAM] z c finger protein ZFP-36 le-21 [SUPFAM] homeobox homology 2e-08 [SUPFAM] unassigned homeobox proteins 2e-08 [PROSITE] CYTOCHROME_C 1 [PROSITE] MYRISTYL 10 [PROSITE] ZINC_FINGER_C2H2 3 [PROSITE] AMIDATION 2 [PROSITE] CAMP_PHOSPHO_SITE 2 [PROSITE] CK2_PHOSPHO_SITE 18 [PROSITE] TYR_PHOSPHO_SITE 3 [PROSITE] PKC_PHOSPHO_SITE 10 [PROSITE] ASN_GLYCOSYLATI0N 7 [PFAM] Zmc finger, C2H2 type [KW] Irregular [KW] 3D [KW] LOW COMPLEXITY 5.65 % SEQ MSQTNFTPDTLAQNEGKAMSYQCSLCKFLSSSFSVLKDHIKQHGQQNEVILMCSECHITS SEG xxxxxxxxxxxxxxx lmeyF SEQ RSQEELEAHVVNDHDNDANIHTQSKAQQCVSPSSSLCRKTTERNETIPDIPVSVDNLQTH SEG lmeyF SEQ TVQTASVAEMGRRKWYAYEQYGMYRCLFCSYTCGQQRMLKTHAWKHAGEVDCSYPIFENE SEG lmeyF SEQ NEPLGLLDΞSAAAAPGGVDAVVIAIGESELSIHNGPSVQVQICSSEQLSSSSPLEQSAER SEG xxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxx... lmeyF SEQ GVHLSQSVTLDPNEEEMLEVISDAEENLIPDSLLTSAQKIIΞSSPNKKGHVNVIVERLPS SEG lmeyF SEQ AEETLSQKRFLMNTEMEEGKDLSLTEAQIGREGMDDVYRADKCTVDIGGLIIGWSSSEKK SEG lmeyF SEQ DELMNKGLATDENAPPGRRRTNSESLRLHSLAAEALVTMPIRAAELTRANLGHYGDINLL SEG lmeyF SEQ DPDTSQRQVDSTLAAYSKMMSPLKNΞSDGLTSLNQSNSTLVALPEGRQELSDGQVKTGIS SEG lmeyF SEQ MSLLTVIEKLRERTDQNASDDDILKELQDNAQCQPNSDTΞLSGNNVVEYIPNAERPYRCR SEG lmeyF TTTEETT SEQ LCHYTSGNKGYIKQHLRVHRQRQPYQCPICEHIADNSKDLESHMIHHCKTRIYQCKQCEE SEG lmeyF TTTCEETTHHHHHHHHHHHHTTCCEEETTTTEEECCHHHHHHHHHHHHCCCCEEETTTTE SEQ SFHYKΞQLRNHEREQHSLPDTLSIATSNEPRISSDTADGKCVQEGNKSSVQKQYRCDVCD SEG lmeyF EECCHHHHHHHHHHHC SEQ YTSTTYVGVRNHRRIHNSDKPYRCSLCGYVCSHPPSLKSHMWKHASDQNYNYEQVNKAIN SEG lmeyF SEQ DAISQSGRVLGKSPGKTQLKSSEESADPVTGSSENAVSΞSELMSQTPSEVLGTNENEKLS SEG lmeyF SEQ PTSNTSYSLEKISSLAPPSMEYCVLLFCCCICGFESTSKENLLDHMKEHEGEIVNIILNK SEG lmeyF SEQ DHNTALNTN SEG lmeyF Prosite for DKFZphtes3_2el2.1 PS00001 104->108 ASN_GLYCOSYLATION PDOC00001 PS00001 445->449 ASN_GLYCOSYLATION PDOC00001 PS00001 454->458 ASN_GLYCOSYLATION PDOC00001 PS00001 457->461 ASN_GLYCOSYLATION PDOC00001 PS00001 497->501 ASN_GLYCOSYLATION PDOC00001 PS00001 646->650 ASN_GLYCOSYLATION PDOC00001 PS00001 784->788 ASN_GLYCOSYLATION PDOC00001 PS00004 98->102 CAMP_PHOSPHO_SITE PDOC00004 PS00004 378->382 CAMP_PHOSPHO_SITE PDOC00004 PS00005 59->62 PKC_PHOSPHO_SITE PDOC00005 PS00005 101->104 PKC_PHOSPHO_SITE PDOC00005 PS00005 306->309 PKC_PHOΞPHO_SITE PDOC00005 PS00005 357->360 PKC_PHOSPHO_SITE PDOC00005 PS00005 385->388 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 425->428 PKC_PHOSPHO_SITE PDOC00005 PS00005 678->681 PKC_PHOSPHO_SITE PDOC00005 PS00005 696->699 PKC_PHOSPHO_SITE PDOC00005 PS00005 726->729 PKC_PHOSPHO_SITE PDOC00005 PS00005 817->820 PKC_PHOSPHO_SITE PDOC00005 PS00006 62->66 CK2_PHOSPHO_SITE PDOC00006 PS00006 106->110 CK2_PHOSPHO_SITE PDOC00006 PS00006 126->130 CK2_PHOSPHO_SITE PDOC00006 PS00006 232->236 CK2_PHOSPHO_SITE PDOC00006 PS00006 262->266 CK2_PHOSPHO_SITE PDOC00006 PS00006 300->304 CK2_PHOSPHO_SITE PDOC00006 PS00006 314->318 CK2_PHOSPHO_ΞITE PDOC00006 PS00006 323->327 CK2_PHOSPHO_SITE PDOC00006 PS00006 355->359 CK2_PHOSPHO_SITE PDOC00006 PS00006 381->385 CK2_PHOSPHO_SITE PDOC00006 PS00006 485->489 CK2_PHOSPHO_SITE PDOC00006 PS00006 499->503 CK2_PHOSPHO_SITE PDOC00006 PS00006 617->621 CK2_PHOSPHO_SITE PDOC00006 PS00006 626->630 CK2_PHOSPHO_SITE PDOC00006 PS00006 741->745 CK2_PHOSPHO_SITE PDOC00006 PS00006 758->762 CK2_PHOSPHO_SITE PDOC00006 PS00006 766->770 CK2_PHOSPHO_SITE PDOC00006 PS00006 817->821 CK2 PHOSPHO SITE PDOC00006 PS00007 331->339 TYR_PHOSPHO_SITE PDOC00007 PS00007 703->711 TYR_PHOSPHO_SITE PDOC00007 PS00007 596->605 TYR_PHOSPHO_SITE PDOC00007 PS00008 142->148 MYRISTYL PDOC00008 PS00008 185->191 MYRISTYL PDOC00008 PS00008 196->202 MYRISTYL PDOC00008 PS00008 241->247 MYRISTYL PDOC00008 PS00008 349->355 MYRISTYL PDOC00008 PΞ00008 473->479 MYRISTYL PDOC00008 PS00008 478->484 MYRISTYL PDOC00008 PS00008 645->651 MYRISTYL PDOC00008 PΞ00008 751->757 MYRISTYL PDOC00008 PS00008 772->778 MYRISTYL PDOC00008 PS00009 130->134 AMIDATION PDOC00009 PS00009 376->380 AMIDATION PDOC00009 PΞ00028 146->167 ZINC_FINGER_C2H2 PDOC00028 PS00028 684->705 ZINC_FINGER_C2H2 PDOC00028 PS00028 595->617 ZINC_FINGER_C2H2 PDOC00028 PS00190 53->59 CYTOCHROME C PDOC00169 Pfam for DKFZphtes3_2el2.1 HMM_NAME Z c finger, C2H2 type HMM *CpwPDCgKtFrrwsNLrRHMR.T.H* C++ C+ T R++++L++H H Query 53 CSE—CHITSRSQEELEAHVVN-DH 74 23.25 (bits) f: 539 t: 559 Target: dkfzphtes3_2el2.1 similarity to fmger proteins Alignment to HMM consensus: Query *CpwPDCgKtFrrwsNLrRHMRTH* C C++T ++ ++H+R+H dkfzphtes3 539 CRL—CHYTSGNKGYIKQHLRVH 559 Query f: 567 t: 587 Target: dkfzphtes3_2el2.1 similarity to finger proteins Alignment to HMM consensus : HMM *CpwPDCgKtFrrwsNLrRHMRTH* CP+ C+ ++ +L+ HM+ H Query 567 CPI—CEHIADNSKDLESHMIHH 587 33.47 (bits) f: 595 t: 616 Target: dkfzphtes3_2el2.1 similarity to fmger proteins Alignment to HMM consensus: Query *CpwPDCgKtFrrwsNLrRHMR.T.H* C+ C+++F ++Ξ+LR+H R H dkfzphtes3 595 CKQ—CEESFHYKSQLRNHERE-QH 616 Query f: 656 t: 676 Target: dkfzphtes3_2el2.1 similarity to fmger proteins Alignment to HMM consensus: HMM *CpwPDCgKtFrrwsNLrRHMRTH* C++ C++T ++ R+H+R+H Query 656 CDV—CDYTSTTYVGVRNHRRIH 676 24.53 (bits) f: 684 t: 704 Target: dkfzphtes3_2el2.1 similarity to finger proteins Alignment to HMM consensus: Query *CpwPDCgKtFrrwsNLrRHMRTH* C+ CG++ +++ +L+ HM H dkfzphtes3 684 CΞL--CGYVCSHPPSLKSHMWKH 704 Query f: 809 t: 829 Target: dkfzphtes3_2el2.1 similarity to fmger proteins Alignment to HMM consensus: HMM *CpwPDCgKtFrrwsNLrRHMRTH* C + CG ++++NL HM+ H Query 809 CCI--CGFESTSKENLLDHMKEH 829 DKFZphtes3_2fl4 group: testes derived DKFZphtes3_2f14 encodes a novel 129 amino acid protein with very weak similarity to human omega protein. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . weak similarity to omega protein complete cDNA, complete eds, 1 EST hit Sequenced by EMBL Locus: unknown Insert length: 2353 bp Poly A stretch at pos. 2341, no polyadenylation signal found 1 GCAGATTCTC CAGGCCCAGC ATCTGCCTCA CCGTGGCCCC CCACAAGCCA 51 AGCGCCTGCC TTTCAGCAGC CTCTACACAC CCAGCTCCTG CCACCCAATG 101 GCTCTTTAGG CCAAGCTCAT ACCTCACGAT GATTTTTCCA GGCCCAACTT 151 TTGTCTCATG GCAACCTTCC CTGGCCAAGT TTCCACCTAT TTCCTGGCAG 201 CCTGGACAGG CCCAGGTCCT GCCACACACT GGCCTCTCTA CGCCCAGCTC 251 ATGCCTCACA GTGGCCTCTC CAGGCCCAGC TCCTGTCCCG GGACATCATC 301 TCCAGGCCCA AAACTTCCTC AAGTCGGCCT CTCCAGGCCC AGTTGCTGCC 351 TCCCGGCATT CTCTCCAGGC CTAGCTCTTC CTCCTGGCTG TATCTACAAG 401 ACCAACTCCT GCCTCACAAC AACCTTTTAT GGCTCAGCTC CTGCCCAACT 451 ACTGCCGGCC TTTGTAGGCC CAAAACTTCC TCAAGTCAAG CTCTTTAGGC 501 CCACCTTCTG CCTTGCAGTG GCCTGTACAG ACCCAGCTCT GGCTTGAGAA 551 CAGCCTCTGC AGGCCCTGCT CTTGCCTCTT AGCTCCCTCT CCAGGCCCAT 601 CTCTTGCCTC ACAGTGGCTT CCGTGGGCCA AGTTCCCGCC TGCCTCCCAG 651 CAGCCTCAAC AGGCCTAGCT CCTCCCTCAC AATGGCTTGT TTAGGTCCAG 701 TTGATGCCTC TGGCAACCTG TCCAGGCCCA GCTCCTGCCT CACACTGGCC 751 TCTCTAGGCC GAGGTCCTTT CTCATACTGG CCTGTTTAGG CCCAGCTCAT 801 TCCTCTTGTC ATCTCTCCAG GCCCAGCTTT TGCCTGTTGT TGGCCTCTAC 851 CTCACAGTGC ACCTTCCAGT CCCACCTCTT GCCTCACCAT GGCCTCCTCT 901 GACCAGGTTC CTGCCTTTCG GCAGCCTCTA CAGGCCTAGC TGCTGCCTCC 951 CAATGGCCTT TGTAGGCCAC GCTCATGCCT CACTGTGGCC TTTCCAGGCC 1001 TAGCTTTCGC TTTTTGGCCA CTCCAGGCCC AGAACTTCCC CCAGTCAGCC 1051 TCTCCAGGCC CAGCTCTTCC TCCCAGCAAC CTCTGCAGGC CCAAATCATC 1101 CTCAAATTGG CCTCTTCTTT CCCAGCTCCT GCCTCCTGGT GGCCTCTGAA 1151 GACCCAAATC GTCCTCCAGT TGGTTTTTCC AGGCCCAGCT CCTGCCTTTT 1201 GGTGGCCTCT CCAGGTGCAA AACTTCCTCC CATCAGCCTG TCCAGGCCCA 1251 GCTCATGCCT CTTGGTGGCC TTCTCAGGCC CTGCTTTTGA CTTGGTGGCC 1301 TCTTCAGGCC CAGAACTTGA ACTCAAGTCA GCCTCTCCAG GCCCAGCTCC 1351 TGCCTTCTTA AGGTCTGTAC AGGCCCAGCC TCTACCTCAC AGCGGACTCT 1401 CCACACCCAG CTCTTGCCTC ACTGTAGCCT CCCCAGTCCA AAACTCCTGC 1451 CTTTTGGCAG CTTCGACAAG CCCAGCTCCT GCCTTTCAAT GACCTCTTTA 1501 GGCCCCGCTC ATTCCTTACA ACGGCCTTTC CAGGCCCAGT TTTTCCCTTT 1551 TGGCGGCCTC TCCAGGCCCA GAACTTCCTC AAGTCGGCCT CTTTAGGCCC 1601 AGTTGCTGCC TCCTGGCATC CTCTGCAGGC CGAGCTCTTC CTCCCTGCTG 1651 TGTCTACAGG CCCAACTCCT GCCTCACAAC AACCTCCTTG GACTCAGCTT 1701 CTGCCCAGCT CCTGGTGGCC TTTGTAGGCT CAAAATTTTC TCAAATCAAG 1751 CTCTCCAGGC CTACTGTCAG CCTCGTGGCA GCCTAAACAG GCCCAGCTCC 1801 TGCCTGACAA TGGCCTCTCC AGGCTTTTCT CCTGCCTCGC AGCAGGCTTT 1851 CCAGGCCCAG CTCTTGCCTC ATGGTGGCCT TCCCCGGCCA TGTTCCTATC 1901 TGACTTCTGG CAGCCTCAAC CGGCCCAGCT TCTGCCTCAC ACTGGCCTCT 1951 CTAGGCCCAG CTCCTTTTTC ACAGTGGCCT CACTACGCCC ATCTCCTACC 2001 TCAGATCTGC CTCCCAAGAC CCAGCTCCTG TCTCATGGTG GTCTCTCTTA 2051 CACCAGCTCC TGCCTCACAA TGGCCTCGTC TGGCCCATCT TCTGCCTCAC 2101 AGTGGCCACT CAAGGCCCAT CTTTTGCCTC ATGGTAGCCT CTTCTGGTTT 2151 TGCTCTTGCC TCACAGTTGC CTCTTCCAGA TCCAGCTTTA AGCCTTTGAT 2201 GGTCAACAGC ATCAAGGAGC CTAAAGCTTC CCTGGACTCT CATTTGTTCA 2251 CTTTACAGCA GAGTGCCTTA GCAAAAACTG TCTCTTAACC TTGAGAGTGG 2301 ATTTCTGACA AATCGATAGT AAATTCTGCC TGTGTGGTTT CAAAAAAAAA 2351 AAA BLAST Results No BLAST result Medlme entries No Medline entry Peptide information for frame 2 ORF from 158 bp to 544 bp; peptide length: 129 Category: similarity to known protein 1 MATFPGQVST YFLAAWTGPG PATHWPLYAQ LMPHSGLSRP SSCPGTSSPG 51 PKLPQVGLSR PSCCLPAFSP GLALPPGCIY KTNSCLTTTF YGSAPAQLLP 101 AFVGPKLPQV KLFRPTFCLA VACTDPALA BLASTP hits Entry 170697 from database PIR: omega protein - human (fragment) Score = 79, P = 2.8e-03, identities = 32/94, positives = 38/94 Alert BLASTP hits for DKFZphtes3_2f14, frame 2 No Alert BLASTP hits found Pedant information for DKFZphtes3_2f14, frame 2 Report for DKFZphtes3_2f1 .2 [LENGTH] 129 [MW] 13421.76 [pi] 9.14 [PROSITE] MYRISTYL 2 [KW] Irregular [KW] LOW COMPLEXITY 10.85 % SEQ MATFPGQVSTYFLAAWTGPGPATHWPLYAQLMPHSGLSRPSSCPGTSSPGPKLPQVGLSR SEG xxxxxxxxxxxxxx PRD cccccccceeehhhhhcccccccccccccccccccccccccccccccccccccccccccc SEQ PSCCLPAFSPGLALPPGCIYKTNSCLTTTFYGSAPAQLLPAFVGPKLPQVKLFRPTFCLA SEG PRD cccccccccccccccccccccccccceeeccccccccccccccccccccccccccccccc SEQ VACTDPALA SEG PRD ccccccccc Prosite for DKFZphtes3_2f14.2 PS00008 6->12 MYRISTYL PDOC00008 PS00008 92->98 MYRISTYL PDOC00008 (No Pfam data available for DKFZphtes3_2f14.2 ) DKFZphtes3_2g7 group: testes derived DKFZphtes3_2g7 encodes a novel 359 ammo acid protein with similarity to neurofiliament proteins . No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . similarity to neurofilament proteins complete cDNA, complete eds, 6 EST hits (5 hits are out of a testis library) Sequenced by EMBL Locus: unknown Insert length: 1613 bp Poly A stretch at pos. 1595, polyadenylation signal at pos. 1557 1 GCCACACAGG CTCCTTGGAG TAAGAGTGTG AGAAACTGGA TGAAGACAGC 51 TGTATTCTTT TGGAAGCGTT CGAGATTGGT CTGTCTCTAC CAACTAAAAA 101 CTTCTAGCTT AAGTGCAGAG ATTTAAGGAG ATCAACAAAA ACTCAGTCTA 151 GACATATTAT GAGGCTGGGA GGGTATCAAC AGACTTGAGT TCTTGTCAGC 201 AAGATCACCT GCTTTTAATA TTGTCCTCAG GGTCTGAGCA CATCTGGAAG 251 TGAGGTCAAT CAAGTTAGAC CCCAAAAACT TTTGTGACAA CAGTGAAGAG 301 GGGAAAATAA ACACACCACA AACATGAACC TCAACCCCCC GACATCTGCT 351 CTTCAGATCG AGGGCAAAGG CAGCCATATT ATGGCTAGAA ATGTAAGCTG 401 CTTTCTAGTC AGGCACACCC CTCATCCCAG AAGAGTCTGC CACATCAAAG 451 GCTTGAATAA CATTCCAATC TGTACTGTGA ATGATGATGA GAATGCATTT 501 GGAACATTGT GGGAAGTTGG CCAGTCTAAC TACTTAGAGA AGAACAGGAT 551 ACCATTTGCC AATTGCAGTT ACCCCCCGAG CACTGCAGTC CAGAAGAGCC 601 CTGTAAGAGG AATGTCGCCA GCCCCAAACG GTGCCAAAGT GCCTCCACGG 651 CCTCATTCTG AGCCCAGTAG AAAAATTAAA GAGTGCTTCA AAACTTCCAG 701 TGAGAATCCC TTAGTAATTA AAAAGGAAGA AATTAAGGCC AAAAGACCAC 751 CATCACCTCC AAAGGCATGC TCTACTCCTG GCTCCTGTTC TTCAGGGATG 801 ACAAGTACCA AGAATGATGT GAAAGCAAAC ACCATTTGCA TACCAAACTA 851 TCTGGATCAG GAAATAAAAA TCCTGGCAAA GCTCTGTAGC ATTTTGCATA 901 CTGATTCTCT GGCAGAAGTT TTACAGTGGC TGCTTCATGC AACTTCAAAA 951 GAAAAAGAGT GGGTCTCAGC TTTGATTCAT TCTGAGCTTG CCGAGATAAA 1001 CCTGTTAACT CATCACAGAA GAAACACCTC AATGGAACCA GCAGCAGAGA 1051 CTGGGAAGCC ACCCACAGTT AAATCACCAC CCACAGTTAA ATTGCCCCCA 1101 AATTTTACTG CAAAATCAAA AGTGCTGACC AGAGATACAG AAGGGGATCA 1151 ACCAACCAGA GTGTCAAGTC AAGGATCTGA AGAAAACAAG GAAGTACCAA 1201 AAGAGGCTGA GCACAAGCCT CCACTACTTA TAAGAAGAAA TAATATGAAA 1251 ATACCTGTTG CAGAATATTT CAGCAAACCA AATTCTCCTC CCAGGCCTAA 1301 CACTCAGGAG AGTGGATCAG CAAAACCAGT GTCAGCAAGG AGTATACAAG 1351 AATACAACCT CTGTCCCCAA AGAGCATGTT ATCCTTCAAC ACACCGGAGG 1401 TAGAAGTTCT AGACTGGGTG AATTCTTTCA TGAATATGAG CTTCACATTT 1451 ACATCATCAA ATTATTTTTC AAATGAATAT TTTTGGTATT GAGGAATCAA 1501 GTGGTCCTCT TTATGGTGGC ACATGTAAAT CTAAAAATAC CTGTATGTAA 1551 TGCTACAAAT AAATATTACT GGAAATGATA TTTCCATTTG TAGTTAAAAA 1601 AAAAAAAAAA AAA BLAST Results No BLAST result Medline entries No Medlme entry Peptide information for frame 3 ORF from 324 bp to 1400 bp; peptide length: 359 Category: similarity to known protein 1 MNLNPPTSAL QIEGKGSHIM ARNVSCFLVR HTPHPRRVCH IKGLNNIPIC 51 TVNDDENAFG TLWEVGQSNY LEKNRIPFAN CSYPPSTAVQ KSPVRGMSPA 101 PNGAKVPPRP HSEPSRKIKE CFKTSSENPL VIKKEEIKAK RPPSPPKACS 151 TPGSCSSGMT STKNDVKANT ICIPNYLDQE IKILAKLCSI LHTDSLAEVL 201 QWLLHATSKE KEWVSALIHS ELAEINLLTH HRRNTSMEPA AETGKPPTVK 251 SPPTVKLPPN FTAKSKVLTR DTEGDQPTRV SSQGSEENKE VPKEAEHKPP 301 LLIRRNNMKI PVAEYFSKPN SPPRPNTQES GSAKPVSARS IQEYNLCPQR 351 ACYPSTHRR BLASTP hits Entry A43427 from database PIR: neurofilament triplet HI protein - rabbit (fragment) Score = 118, P = 5.6e-04, identities = 79/290, positives = 110/290 Entry RNNFH_1 from database TREMBL: Rat high molecular weight neurofilament (NF-H) protein mRNA, 3' end. Score = 115, P = 9.5e-04, identities = 69/281, positives = 100/281 Entry B43427 from database PIR: neurofilament protein H form H2 (repetitive region) - rabbit (fragment) Score = 111, P = 1.3e-03, identities = 64/269, positives = 102/269 Alert BLASTP hits for DKFZphtes3_2g7, frame 3 No Alert BLASTP hits found Pedant information for DKFZphtes3_2g7, frame 3 Report for DKFZphtes3_2g7.3 [LENGTH] 359 [MW] 39725.53 [pi] 9.45 [PROSITE] MYRISTYL 3 [PROSITE] CAMP_PHOSPHO_SITE 1 [PROSITE] CK2_PHOSPHO_SITE 9 [PROSITE] PKC_PHOSPHO_SITE 10 [PROSITE] ASN_GLYCOSYLATION 4 [KW] Alpha_Beta [KW] LOW_COMPLEXITY 4.18 % SEQ MNLNPPTSALQIEGKGSHIMARNVSCFLVRHTPHPRRVCHIKGLNNIPICTVNDDENAFG SEG PRD ccccccccceeecccccceeeeccceeeeecccccccccccccccccccccccccccccc SEQ TLWEVGQSNYLEKNRIPFANCSYPPSTAVQKSPVRGMSPAPNGAKVPPRPHSEPSRKIKE SEG PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccchhhhhh SEQ CFKTSSENPLVIKKEEIKAKRPPSPPKACSTPGSCSSGMTSTKNDVKANTICIPNYLDQE SEG PRD hcccccccceeeehhhhhhccccccccccccccccccccccccccccceeeeccccchhh SEQ IKILAKLCSILHTDSLAEVLQWLLHATSKEKEWVSALIHSELAEINLLTHHRRNTSMEPA SEG PRD hhhhhhhhhhcccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccccccc SEQ AETGKPPTVKSPPTVKLPPNFTAKSKVLTRDTEGDQPTRVSSQGSEENKEVPKEAEHKPP SEG .... xxxxxxxxxxxxxx PRD cccccccccccccccccccccccceeeeecccccccceeeeccccccccccccccccccc SEQ LLIRRNNMKIPVAEYFSKPNSPPRPNTQESGSAKPVSARSIQEYNLCPQRACYPΞTHRR SEG PRD eeeeccccccceeeeecccccccccccccccccccchhhhhhccccccccccccccccc Prosite for DKFZphtes3_2g7.3 PS00001 23->27 ASN_GLYCOSYLATION PDOC00001 PS00001 80->84 ASN_GLYCOSYLATION PDOC00001 PS00001 234->238 ASN GLYCOSYLATION PDOC00001 ^1 σ D α σ o α σ α o σ o a a D σ σ σ O o o o o o O o o o O o o o o o o o o o o o o o DKFZphtes3 2hl group: transmembrane protein DKFZphtes3_2hl encodes a novel 116 amino acid protein with weak similarity to C. elegans cosmid C13F10. The novel protein contains 1 transmembrane region. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes and as a new marker for testicular cells. similarity to C. elegans C13F10.5 TRANSMEMBRANE 1 Sequenced by EMBL Locus: /map="2" Insert length: 1156 bp Poly A stretch at pos. 1143, polyadenylation signal at pos. 1121 1 GGCCATCAAA ATAACTAAAC CATGTCATTT GGAGCAACAA AGCCACTGCG 51 GCCTCCATTT GGGCCAAGCT CTGACTGCAA TGATGCCTCT GCCCCGACCC 101 GGGCCTCGCT GTGACTGACA ATGCCGCTGC ATCTTTTCAG CAGTCATTGA 151 TGAGGAAGTA TCTACATCCT CCTTCCCACT ACCAGATTTT GCTTGGAGAA 201 AAGCAGTTTC CTGAAATAAT TCTGTGACGA GCTTCTTCCA CATTAGGACA 251 AAAATGCTGG AAGCGGCTCA GCCCCAGGGC AGCACATCAG AGACACCATG 301 GAACACAGCC ATTCCTCTGC CGTCGTGCTG GGACCAGTCT TTCCTGACCA 351 ATATCACCTT CTTGAAGGTT CTTCTCTGGT TGGTCCTGCT GGGACTGTTT 401 GTGGAACTGG AATTTGGCCT GGCATATTTT GTCCTGTCCT TGTTCTATTG 451 GATGTACGTC GGGACACGAG GCCCTGAAGA GAAGAAAGAG GGAGAGAAGA 501 GCGCCTACTC TGTGTTCAAT CCAGGCTGTG AAGCCATCCA GGGCACCCTG 551 ACTGCAGAGC AGTTGGAGCG CGAGTTACAG TTGAGACCCC TGGCAGGGAG 601 ATAGGACCCA GCTGTGCTGT CATGCAGCTA ACCTCTGATG TGGTCTTCCT 651 CACCATTGGC TATGGATTTG ATTTCAGGTG TATAGGACTA AGGGCAGCTT 701 GCGGGTTAGC TCTGTGACTG CATAGTTTTT CTACCTTCTT TCCCTGATCT 751 TTTGCTGCCA TTTGATCTTT GATAGTTTTG GTGAAACTCT CTAAAATACA 801 TTCACTGTGG GTCCGACGCA ATTTATAAAA ATTATGTACT CAAGAAGGGA 851 GACCTGTTTG TTTCATTTCT CATCTGTTTG GGAGATGATT TTAGAGCACT 901 AGAAAGGCAC TGGGGAGATT CTCAGCTTAA AACATCCAGC AGTTTGAAGT 951 ATGATTAGGT ACATCAGGGC TGCATTGTCA ATGTTCTCTT TAAGTCTTTT 1001 AACATTTATA GCAATTTTTT TTTTCCCGGA GAGTTTAGGT TGCAAGTTTT 1051 GGGTTTCTTG TTTGTTTTTG TTTTGCTTCC TGCTTTAATT CTTTAATTTT 1101 CAGTCATTAC TGGTATTGAA AAATAAAATA TCTTTAAAAC ATCAAAAAAA 1151 AAAAAA BLAST Results Entry HS313307 from database EMBL: human STS SHGC-16715. Score = 1222, P = 1.4e-48, identities = 248/251 Medline entries No Medlme entry Peptide information for frame 2 ORF from 254 bp to 601 bp; peptide length: 116 Category: similarity to unknown protein 1 MLEAAQPQGS TSETPWNTAI PLPSCWDQSF LTNITFLKVL LWLVLLGLFV 51 ELEFGLAYFV LSLFYWMYVG TRGPEEKKEG EKSAYSVFNP GCEAIQGTLT 101 AEQLERELQL RPLAGR BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_2hl, frame 2 TREMBL :CEUC13F10_2 gene: "C13F10.5"; Caenorhabditis elegans cosmid C13F10., N = 1, Score = 141, P = 8.2e-10 >TREMBL:CEUC13F10_2 gene: "C13F10.5"; Caenorhabditis elegans cosmid C13F10. Length = 171 HSPs: Score = 141 (21.2 bits), Expect = 8.2e-10, P = 8.2e-10 Identities = 32/82 (39%), Positives = 52/82 (63%) Query: 27 DQSFLTNITFLKVLLWLVLLGLFVELEFGLAYFVLSLFYWMYVGTRGPEEKKEGEKSAYS 86 +QS ++ T + V++++V L ++FG +F+LSL + Y T G ++ GE SAYS Sbjct: 90 EQSVVS—TRIAVVVYVVGQALAAWVQFGAVFFILSLILFTYWNT-G—RRRRGEMSAYS 144 Query: 87 VFNPGCEAIQGTLTAEQLEREL 108 VFN CE + G++TAE ER++ Sbjct: 145 VFNDNCERLAGSMTAEHFERDM 166 Pedant information for DKFZphtes3_2hl, frame 2 Report for DKFZphtes3_2hl .2 [LENGTH] 116 [MW] 13092.19 [pi] 4.64 [PROSITE] MYRISTYL 1 [PROSITE] CK2 PHOSPHO SITE 2 [PROSITE] TYR PHOSPHO SITE 2 [PROSITE] ASN GLYCOSYLATION 1 [KW] TRANSMEMBRANE 1 [KW] LOW COMPLEXITY 32 .76 % SEQ MLEAAQPQGSTSETPWNTAIPLPSCWDQSFLTNITFLKVLLWLVLLGLFVELEFGLAYFV SEG xxxxxxxxxxxxxxxxxxxxx .... PRD ccccccccccccccccccccccccccccccccchhhhhhhhhhhhhhhhhhhhhchhhhh MEM MMMMMMMMMMMMMMMMM SEQ LSLFYWMYVGTRGPEEKKEGEKSAYSVFNPGCEAIQGTLTAEQLERELQLRPLAGR SEG xxxxxxxxxxxxxxxxx .. PRD hhhhhhhhcccccchhhhhcccceeeecccccccccccchhhhhhhhhhccccccc MEM Prosite for DKFZphtes3_2hl .2 PS00001 33->37 ASN_GLYCOSYLATION PDOC00001 PS00006 10->14 CK2_PHOSPHO_SITE PDOC00006 PS00006 24->28 CK2_PHOSPHO_SITE PDOC00006 PS00007 78->86 TYR_PHOSPHO_SITE PDOC00007 PS00007 77->86 TYR_PHOSPHO_SITE PDOC00007 PS00008 97->103 MYRISTYL PDOC00008 (No Pfam data available for DKFZphtes3 2hl.2) DKFZphtes3_2hl5 group: testes derived DKFZphtes3_2hl5 encodes a novel 855 amino acid protein with very weak similarity to S. pombe cdc23. No informative BLAST results; no predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . similarity to cdc23 complete cDNA, complete eds, EST hits Sequenced by EMBL Locus : unknown Insert length: 4619 bp Poly A stretch at pos. 4598, polyadenylation signal at pos. 4589 1 GAAGGCGTCC CGGCATCGGC CAAGATTCTA CATTGCTCAT CTGGGCATCT 51 GAGCCTCCTT CGAAGTTTCC TGTCACAACT GTCCTCTTGA CAGCATGGAT 101 GAGGAGGAAG ACAATCTGTC TCTGCTGACC GCACTGCTGG AAGAAAATGA 151 GTCAGCCTTG GATTGTAATT CAGAAGAAAA TAACTTCTTG ACGCGGGAAA 201 ATGGCGAGCC CGACGCATTT GATGAGCTCT TTGATGCCGA CGGCGACGGT 251 GAATCTTATA CAGAAGAGGC TGATGATGGA GAAACAGGAG AGACAAGAGA 301 CGAAAAGGAA AATCTGGCCA CTCTCTTTGG AGATATGGAG GACTTAACAG 351 ATGAAGAAGA AGTTCCCGCA TCACAGTCAA CTGAAAATAG GGTCCTCCCT 401 GCTCCTGCCC CCAGGCGAGA GAAAACGAAT GAAGAGTTGC AAGAGGAATT 451 AAGGAATTTG CAAGAGCAAA TGAAGGCCTT ACAAGAGCAG CTAAAAGTAA 501 CAACAATTAA ACAGACAGCA AGCCCAGCCC GTCTGCAAAA ATCCCCTGAG 551 AAGTCTCCCC GGCCACCTCT TAAGGAGAGG AGAGTTCAGA GAATTCAGGA 601 GTCAACATGC TTTTCTGCGG AGCTTGATGT CCCTGCGCTA CCAAGAACCA 651 AGAGGGTGGC TCGAACACCA AAGCCTTCAC CTCCAGATCC CAAAAGCTCA 701 TCTTCAAGGA TGACAAGTGC ACCCTCCCAA CCCCTACAGA CGATTTCTCG 751 GAACAAACCT AGTGGGATAA CTAGAGGTCA AATTGTGGGG ACCCCAGGAA 801 GTTCTGGGGA AACGACTCAA CCCATCTGTG TGGAAGCCTT CTCTGGTCTG 851 CGGCTCAGGC GGCCTCGAGT ATCCTCCACA GAAATGAACA AGAAAATGAC 901 CGGCCGAAAA CTGATCAGAC TGTCTCAGAT CAAGGAAAAG ATGGCCAGAG 951 AGAAGCTGGA AGAAATAGAT TGGGTGACAT TTGGGGTTAT ATTGAAGAAG 1001 GTTACGCCAC AGAGTGTGAA TAGTGGAAAA ACCTTCAGCA TATGGAAACT 1051 GAATGATCTT CGTGACCTGA CACAATGTGT GTCCTTGTTC TTATTTGGAG 1101 AAGTTCACAA AGCGCTCTGG AAGACGGAGC AGGGGACTGT CGTAGGGATC 1151 CTCAATGCCA ACCCCATGAA GCCCAAGGAT GGTTCAGAGG AGGTGTGTTT 1201 ATCTATCGAT CATCCTCAGA AGGTCTTAAT TATGGGTGAA GCTCTTGACC 1251 TGGGAACCTG TAAAGCCAAG AAGAAGAATG GAGAGCCGTG CACGCAGACT 1301 GTGAATTTGC GTGACTGTGA GTACTGTCAG TACCATGTCC AGGCTCAGTA 1351 CAAGAAGCTC AGTGCAAAGC GTGCGGATCT GCAGTCCACC TTCTCTGGAG 1401 GACGAATTCC AAAGAAGTTT GCCCGCAGAG GCACCAGCCT CAAAGAACGG 1451 CTGTGCCAAG ATGGCTTTTA CTACGGAGGG GTTTCTTCTG CCTCGTATGC 1501 AGCTTCAATT GCAGCAGCTG TGGCTCCTAA GAAGAAGATT CAAACCACTC 1551 TGAGTAATCT GGTTGTTAAG GGCACAAACT TGATCATCCA GGAAACACGG 1601 CAAAAACTCG GAATACCCCA GAAGAGCCTG TCTTGCTCTG AGGAGTTCAA 1651 GGAACTGATG GACCTGCCGA CGTGTGGAGC CAGGAACTTA AAACAACATT 1701 TAGCCAAAGC CTCAGCTTCA GGGATTATGG GGAGCCCAAA ACCAGCCATC 1751 AAGTCCATCT CGGCCTCAGC ACTCTTGAAG CAACAGAAGC AGCGGATGTT 1801 GGAGATGAGG AGAAGGAAAT CAGAAGAAAT ACAGAAGCGA TTTCTGCAGA 1851 GCTCAAGTGA AGTTGAGAGC CCAGCTGTGC CATCTTCATC AAGACAGCCC 1901 CCTGCTCAGC CTCCACGGAC AGGATCCGAG TTCCCCAGGC TGGAGGGAGC 1951 CCCGGCCACA ATGACGCCCA AGCTGGGGCG AGGTGTCTTG GAAGGAGATG 2001 ATGTTCTCTT TTATGATGAG TCACCACCAC CAAGACCAAA ACTGAGTGCT 2051 TTAGCAGAAG CCAAAAAGTT AGCTGCTATC ACCAAATTAA GGGCAAAAGG 2101 CCAGGTTCTT ACAAAAACAA ACCCAAACAG CATTAAGAAG AAACAAAAGG 2151 ACCCTCAGGA CATCCTGGAG GTGAAGGAAC GTGTAGAAAA AAACACCATG 2201 TTTTCTTCTC AAGCTGAGGA TGAATTGGAG CCTGCCAGGA AAAAAAGGAG 2251 AGAACAACTT GCCTATCTGG AATCTGAGGA ATTTCAGAAA ATCCTAAAAG 2301 CAAAATCAAA ACACACAGGC ATCCTGAAAG AGGCCGAGGC TGAGATGCAG 2351 GAGCGCTACT TTGAGCCACT GGTGAAAAAA GAACAAATGG AAGAAAAGAT 2401 GAGAAACATC AGAGAAGTGA AGTGCCGTGT CGTGACATGC AAGACGTGCG 2451 CCTATACCCA CTTCAAGCTG CTGGAGACCT GCGTCAGTGA GCAGCATGAA 2501 TACCACTGGC ATGATGGTGT GAAGAGGTTT TTCAAATGTC CCTGTGGAAA 2551 CAGAAGCATC TCCTTGGACA GACTCCCGAA CAAGCACTGC AGTAACTGTG 2601 GCCTCTACAA ATGGGAACGG GACGGAATGC TAAAGGTATG CCATTTGCGT 2651 ACTAATTTTT GACTCCTTTT AGTGACCCAT GCTAATAATG TGGAACCATC 2701 TCCTATTAAA ATATTTTCAT TTTTCTAGGA AAAGACTGGT CCAAAGATAG 2751 GAGGAGAAAC TCTGTTACCA AGAGGAGAAG AACATGCTAA ATTTCTGAAC 2801 AGCCTTAAAT AACCCGAACT TCAGACATTT TCCCACAGAC TTCCTGGCCT 2851 CCTGTGACTC TGGAAAGCAA AGGATTGGCT GTGTATTGTC CATTGATTCC 2901 TGATTGACGC CGTCAAAAAC AAATGCTTGT TAAGCCCATA AGCTTTGCCT 2951 GCTTACTTTC TGCCATTGGG TTGGTTTGAT ACCACATTTA ACATTGACAT 3001 TTAAGTGGAA AACCAAGTTA TCATTGTCTT TTCTAAGCTC AGTGTGGATG 3051 ATTGCATTAC TTCATTCACT GAAGTTTTTG CCCAAAAATT GGAAGGTAAA 3101 CAGAGAGCTA TGTTTCTGTA TCTTTTGGTT ATAGAGTGTT CACTTCTTTA 3151 TCATAACAAA ATTCTAGTGT TTATACGAAC ACCCAGAGGC AAAAGAATTT 3201 GGCTTAATTC TCACTCCAGG TAAGTAGCTT AACTTCTGGG CTTCAGTTTT 3251 CTCATCTGTA AAATCAGGAA GATTGGACTA AGTGATCCTG AAATGTATTT 3301 TTTAGCACTG GATTTCTACA AATAATAAAA CTTTCCCATC TAGATAATGA 3351 TGATCACATA GTCTTGATGT ACGGACATTA AAAGCCAGAT TTCTTCATTC 3401 AATTCTGTTA TCTCTGTTTT ACTCTTTGAA ATTGATCAAG CCACTGAATC 3451 ACTTTGCATT TCAGTTTATA TATAGAGAGA GAAAGAAGGC TGTCTGCTCT 3501 TACATTATTG TGGAGCCCTG TGATAGAAAT ATGTAAAATC TCATATTATT 3551 TTTTTTTTAA TTTTTTTATT TTTTATGACA GGGTCTCACT ATGTCACCCT 3601 GGCTGGAGTG CAGTAGTGCG ATCGCGGCAC ACTGCAGCCT TGGCTTCCCT 3651 GGGCTCAAGC AGTCCTCCCA CCTCAGTCTC CCAAATAGCT AGGACTACAG 3701 GCGTGCGTGA CCAAGCCCAG CTAATTTTTG CATTTTTTGT AGAGATGGGG 3751 TTTTGCCATG TTGCTCAGGC TGGTCTCAAA CTCCTGAGCA CTAGCAATCC 3801 ACCCACCTCT GTTTCCAAAA AAAAAAAAAA AATGAAAGGT CAACCCCTAT 3851 GCAAATTACC ACAGCAAAGG TTTCATTCAG GAGATTCTTC CATCTGGGCA 3901 ACCTGGTTTT CCAAATATCA TTTGACCTAA GTGAATGTTG ATACTAGCTA 3951 AAGATTGGGT AAATTGGTTG AATTATTGTA TTGAAGCTTG AGCTGTAGCT 4001 AAAAGTAATT TAGGTTTCCC CTAAGATGTT ATTATGTTAG GGACATAACA 4051 CTTTTGGGAG GTTGTTGTGG GAGATGGTTG ATTTAGGTTT TCAAAAGCTA 4101 GAAATAAAAT TTACATGCCT TAGATTTCAT AAAATTCTGC TCTAATTGGG 4151 TGGAAGGTGC TGTATCTAAC TTGTGTTCCT CCTAAGGTTA TGTCCTAATA 4201 ACTATTCTTT TAGGAGTATA CTTCTACTTT ATAGAAGGTT GCTTTTCTTT 4251 TTAATTTTTT CTAACAAAGA AAAGAATAAA GTATTTATTA ATAAGAACCA 4301 GAAAGCACTT GAAACTGATG TTTTTAATGG CTCATTTAGG GTAGATTTAT 4351 TTATCTCATT AACTTAAAAC AGCTATGTGT ATGAAATAGG TCACAACAGA 4401 ACTTGAACAC CAGGTTGGTG TCTGAGCAAT CCCTTTCTTA TGGGAAAAAC 4451 AATGTTCTTG TTTGAACAGA GGGTATCATT GCAGTCAGTA TTCACGTGTA 4501 TATTGTTATA TAAGTTGTAT AATATGCTTG TAAAGGCTGA GGGTGAGCTG 4551 TATCTGGATG CCTTTTTACA ATTTGATTTT AACTTTTAAA ATAAATTTAA 4601 AACATAAAAA AAAAAAAAA BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 2 ORF from 95 bp to 2659 bp; peptide length: 855 Category: similarity to known protein Classification: Cell division 1 MDEEEDNLSL LTALLEENES ALDCNSEENN FLTRENGEPD AFDELFDADG 51 DGESYTEEAD DGETGETRDE KENLATLFGD MEDLTDEEEV PASQSTENRV 101 LPAPAPRREK TNEELQEELR NLQEQMKALQ EQLKVTTIKQ TASPARLQKS 151 PEKSPRPPLK ERRVQRIQES TCFSAELDVP ALPRTKRVAR TPKPSPPDPK 201 SSSSRMTSAP SQPLQTISRN KPSGITRGQI VGTPGSSGET TQPICVEAFΞ 251 GLRLRRPRVS STEMNKKMTG RKLIRLSQIK EKMAREKLEE IDWVTFGVIL 301 KKVTPQSVNS GKTFSIWKLN DLRDLTQCVS LFLFGEVHKA LWKTEQGTVV 351 GILNANPMKP KDGSEEVCLS IDHPQKVLIM GEALDLGTCK AKKKNGEPCT 401 QTVNLRDCEY CQYHVQAQYK KLSAKRADLQ STFSGGRIPK KFARRGTSLK 451 ERLCQDGFYY GGVSSASYAA SIAAAVAPKK KIQTTLSNLV VKGTNLIIQE 501 TRQKLGIPQK SLSCSEEFKE LMDLPTCGAR NLKQHLAKAS ASGIMGSPKP 551 AIKSISASAL LKQQKQRMLE MRRRKSEEIQ KRFLQSSSEV ESPAVPSSSR 601 QPPAQPPRTG ΞEFPRLEGAP ATMTPKLGRG VLEGDDVLFY DESPPPRPKL 651 SALAEAKKLA AITKLRAKGQ VLTKTNPNSI KKKQKDPQDI LEVKERVEKN 701 TMFSΞQAEDE LEPARKKRRE QLAYLESEEF QKILKAKSKH TGILKEAEAE 751 MQERYFEPLV KKEQMEEKMR NIREVKCRVV TCKTCAYTHF KLLETCVSEQ 801 HEYHWHDGVK RFFKCPCGNR SISLDRLPNK HCSNCGLYKW ERDGMLKVCH 851 LRTNF BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_2hl5, frame 2 TREMBLNEW: SPBC1347_10 gene: "cdc23"; "SPBC1347.10"; product: "cell division cycle protein 23"; S. pombe chromosome II cosmid C1347., N = 2, Score = 284, P = 7e-21 PIR:S48384 DNA43 protein - yeast (Saccharomyces cerevisiae), N = 2, Score = 203, P = 7e-12 TREMBL :SCDNA52A_1 gene: "DNA52"; Saccharomyces cerevisiae DNA52 gene, complete eds., N = 2, Score = 201, P = 7.9e-12 TREMBLNEW:AC006234_6 gene: "F5H14.6"; Arabidopsis thaliana chromosome II BAC F5H14 genomic sequence, complete sequence., N = 2, Score = 211, P = 1.7e-15 PIR:S48384 DNA43 protein - yeast (Saccharomyces cerevisiae), N = 2, Score = 203, P = 7.2e-12 >TREMBLNEW:SPBC1347_10 gene: "cdc23"; "SPBC1347.10"; product: "cell division cycle protein 23"; S. pombe chromosome II cosmid cl347. Length = 593 HSPs: Score = 284 (42.6 bits), Expect = 7.0e-21, Sum P(2) = 7.0e-21 Identities = 97/383 (25%), Positives = 186/383 (48%) Query: 109 EKTNEELQEELRNLQEQMKALQEQLKVTTIKQTASPARLQKSPEKSPRPPLKERRVQRIQ 168 E+ + +L+E + LQ Q+ +QE+ ++ + ++ AS + + PR P ++ RV + Sbjct: 8 EENDLDLEE—KRLQRQLNEIQEKKRLRSAQKEASSENAEVI—QVPRSPPQQVRVLTVS 63 Query: 169 ESTCFSAE LDVPALPRTKRVARTPKPSPPDPKSSSSRMTSAPSQP LQTIS 218 + + L + K V+ P P PK R+ A +Q L+T+ Sbjct: 64 SPSKLKSPKRLILGIDKGKTGKDVSLGKGPRGPLPKPFHERLAEARNQERKRSDKLKTMK 123 Query: 219 RNKPSGITRGQIVGTPGSSGETTQPI-C—VEAFSGLRLRRPRVSSTEMNKKMTGRKLIR 275 +N+ R + + G S E P+ C ++ +S + +Ξ + + G ++ Sbjct: 124 KNRKQSFQRKRNILEDGKΞEEEKFPMKCDEIDPYSRQAIVIRYISDEVAKENIGGNQVYL 183 Query: 276 LSQIKEKMAREKLE—EID-WVTFGVILKKV-TPQSVNSGKTFSIWKLNDLRDLTQCVSL 331 + Q+ + + K E E+D +V G++ T ++VN K + + L DL+ +C Sbjct: 184 IHQLLKLVRAPKFEAPEVDNYVVMGIVASNSGTRETVNGNK-YCMLTLTDLKWQLEC 239 Query: 332 FLFGEVHKALWKTEQGTVVGILNANPMKPKDGS-EEVCLSIDHPQKVLI-MGEALDLGTC 389 FLFG+ + WK + GTV+ +LN +KPK+ L +D VL+ +G + LG C Sbjct: 240 FLFGKAFERYWKIQSGTVIALLNPEVLKPKNPDIGRFSLKLDSEYDVLLEIGRSKHLGYC 299 Query: 390 KAKKKNGEPCTQTVNLRDCEYCQYHVQAQYKKLSAKRADLQSTFSGGRIPKKFARRGTSL 449 +++K+GE C ++ R + C+YHV ++ + R + S+ + P+ ARR Sbjct: 300 SSRRKSGELCKHWLDKRAGDVCEYHVDLAVQRSMSTRTEFASSMATMHEPR—ARR 353 Query: 450 KERLCQDGF—YYGGVSSASYAASIAAAVAPKKKIQT 484 ++R GF Y+ G ++ ++A + +QT Sbjct: 354 EKRFRGQGFQGYFAGEKYSAIPNAVAGLYDAEDAVQT 390 Score = 41 (6.2 bits), Expect = 7.0e-21, Sum P(2) = 7.0e-21 Identities = 12/43 (27%), Positives = 17/43 (39%) Query: 453 LCQDGFYYGGVSSASYAASIAAAVAPKKKIQTTLSNLVVKGTN 495 L +D S AS A++ K + SN + GTN Sb j ct: 465 LSKDSEIDSSTKKPSVLASFNASIMNPKSSLPSFSNSAILGTN 507 Score = 40 (6.0 bits), Expect = 8.9e-21, Sum P(2) = 8.9e-21 Identities = 13/26 (50%), Positives = 18/26 (69%) Query: 536 LAKASASGIMGSPKPAIKSISASALL 561 LA +AS IM +PK ++ S S SA+L Sbjct: 481 LASFNAS-IM-NPKSSLPSFSNSAIL 504 Pedant information for DKFZphtes3_2hl5, frame 2 Report for DKFZphtes3_2hl5.2 [LENGTH] 855 [MW] 96135.01 [pi] 8.96 [HOMOL] TREMBLNEW: SPBC1347_10 gene: "cdc23"; "SPBC1347.10"; product: "cell division cycle protein 23"; S. pombe chromosome II cosmid C1347. 5e-16 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YIL150c] le-11 [FUNCAT] 03.16 dna synthesis and replication [S. cerevisiae, YIL150c] le-11 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YIL150c] le-11 [KW] Alpha_Beta [KW] LOW_COMPLEXITY 12.05 % [KW] COILED COIL 4.21 % SEQ MDEEEDNLSLLTALLEENESALDCNSEENNFLTRENGEPDAFDELFDADGDGESYTEEAD SEG xxxxx PRD cccchhhhhhhhhhhhhhhhccccccccceeeeccccccccceeeecccccccceeeeec COILS SEQ DGETGETRDEKENLATLFGDMEDLTDEEEVPASQSTENRVLPAPAPRREKTNEELQEELR SEG xxxxxxxxxxxx xxxxxxxxx PRD cccccccccccchhhhhhcccccccceeeccccccccccccccccccchhhhhhhhhhhh COILS CCCCCCCCCCCCCC SEQ NLQEQMKALQEQLKVTTIKQTAΞPARLQKSPEKSPRPPLKERRVQRIQESTCFSAELDVP SEG xxxxx PRD hhhhhhhhhhhhhhhhhhhhhcccccccccccccccccccceeeeecccccccccccccc COILS CCCCCCCCCCCCCCCCCCCCCC SEQ ALPRTKRVARTPKPSPPDPKSSSΞRMTSAPSQPLQTISRNKPSGITRGQIVGTPGSSGET SEG xxxxxxxxxxxxx PRD cccccceeeecccccccccccchhhhhhhccccchhhhhhccccccceeeeecccccccc COILS SEQ TQPICVEAFSGLRLRRPRVSSTEMNKKMTGRKLIRLSQIKEKMAREKLEEIDWVTFGVIL SEG PRD cccccccccchhhhhhhcccchhhhhhhhhhhhhhhhhhhhhhhhhhhhccceeeeeeee COILS SEQ KKVTPQSVNSGKTFSIWKLNDLRDLTQCVSLFLFGEVHKALWKTEQGTVVGILNANPMKP SEG PRD cccccccccccceeeeeeeccchhhhhhheeeeecchhhhhhhhccceeeeecccccccc COILS SEQ KDGSEEVCLSIDHPQKVLIMGEALDLGTCKAKKKNGEPCTQTVNLRDCEYCQYHVQAQYK SEG PRD ccccceeeeecccccceeeccccccccccccccccccccceeecccccccchhhhhhhhh COILS SEQ KLSAKRADLQSTFSGGRIPKKFARRGTSLKERLCQDGFYYGGVSSASYAASIAAAVAPKK SEG xxxxxxxxxxxxxxxxxxx ... PRD hhhhhhhhhhhhccccccccccccccchhhhhhhccccccccccchhhhhhhhhhhhcch COILS SEQ KIQTTLSNLVVKGTNLIIQETRQKLGIPQKSLSCSEEFKELMDLPTCGARNLKQHLAKAS SEG PRD hhhhhhheeecccceeeehhhhhhhcccccccchhhhhhhhhhccccccchhhhhhhhhh COILS SEQ AΞGIMGSPKPAIKSISAΞALLKQQKQRMLEMRRRKSEEIQKRFLQSSSEVESPAVPSSSR SEG xxxxxxxxxxxxxxx PRD hhcccccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccccccccccccc COILS SEQ QPPAQPPRTGSEFPRLEGAPATMTPKLGRGVLEGDDVLFYDESPPPRPKLSALAEAKKLA SEG xxxxxxxx xxxxxxxxxxxx PRD ccccccccccccccccccccccccccccccccccceeeeeccccccchhhhhhhhhhhhh COILS SEQ AITKLRAKGQVLTKTNPNSIKKKQKDPQDILEVKERVEKNTMFSΞQAEDELEPARKKRRE SEG xxxxx PRD hhhhhhhhheeeeecccccccccccccchhhhhhhhhhhhccchhhhhhhhhhhhhhhhh COILS SEQ QLAYLESEEFQKILKAKSKHTGILKEAEAEMQERYFEPLVKKEQMEEKMRNIREVKCRVV SEG PRD hhhhhhhhhhhhhhhhhcccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhheee COILS SEQ TCKTCAYTHFKLLETCVSEQHEYHWHDGVKRFFKCPCGNRSISLDRLPNKHCSNCGLYKW SEG PRD eeecceeeeeeecccceeeccccccccceeeeeecccccccccccccccccccccceeec COILS SEQ ERDGMLKVCHLRTNF SEG PRD ccccccccccccccc COILS (No Prosite data available for DKFZphtes3_2hl5.2) (No Pfam data available for DKFZphtes3_2hl5.2) DKFZphtes3_2ι5 group: testes derived DKFZphtes3_2ι5 encodes a novel 151 amino acid protein with weak similarity to. C. elegans cosmid F20D12.3 No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . similarity to C. elegans F20D12.3 many ATGs in front of the start of the ORF, unspliced intron in 5' region? Sequenced by EMBL Locus : unknown Insert length: 2142 bp Poly A stretch at pos. 2121, polyadenylation signal at pos. 2102 1 GCAGTAAATA TGATATGAAA GAATTCTCTA ACTTGGGGGT GGCTTGTAAC 51 CTGTAATAAA AATATTGCTA AAATACCTTC TCTCACTTTG AAAAAGCATC 101 TGAGCAATCC TCAGTTATTG GTGAATTCTT ACCAGTGTTT AATTCCTCTC 151 TTTCCGTTAT GGTCTTAGTG TGGTTGTCCT GGTGTAGTAT TTCAAGAGGA 201 ACCTGCAGCA AGATGAAAAG AGAGTGGGAC TTGGAGCTAA GAACGTTTTT 251 GGCTTTAAGT GCTACGTTAA CTCATTAAAT TCTTAGTGAT CTTGGGGAAG 301 TCCCCTCACC AGTGTGAGCC TCAGTTTTCT TATCTAATAA GTAAGGATAA 351 TCTTACCCAC CTTATTGCGG GGGCCCGAGG ATTACATGAT TGGTGTAACA 401 GTAGCACCTT GTACATTTGA AAGGACTAAT ACCAGTGGAC TTTAACCTTG 451 GCTGGGCTTT GGAATTCTTG GTGGGACTTT TTAATCATGT AGATTCTCAG 501 GCCCCTGCCT GGCCTGTGGA ACCACAGACT CTATAGGTGG GCCCTTCCAG 551 AAGGCCTCAT GGGTGGTTCT CATGTGGAAC CTGTGTTGCA AGCCACTGCA 601 TGGTGTTACT GCTATTAACA TTAAAACTTA TATTTTCCTT ATTGTGTGGA 651 TATATCTGTG GTGTTTGCCC ATGTATACTT CATTTTACAT TTCTTAAAGA 701 ATAGAATGGA ATGGTTTTAA GCACGCTACA TTGTCCAGGT TATACCCACA 751 GAAGAGCTGT TGTGTAACAG AATCAGCATC ATACCTGAAT CATTTGTACA 801 TTGCATATAA GACTATGTCT AAGTAGAAGA TGCTATGAAA TCATGTCTGC 851 TGTGGGGCCA GGCATAATTA TGAATGTTAC TTAAGAGCAT AGGTGAGGTG 901 AGAAAAGGGA ATGTGACTAG TGTTTTAGTA TTTTCTTGGT GTGGGATGAA 951 GTATAATTCT TTTTTTTTTT TCTCAACAAA GCAGTAAAAC TAGAAAGAAG 1001 GAGAACTCTT CCCTCAAGAA TGGCTGTACC TTCATATCTA GAGGCACATT 1051 AAAAAAAAGA ACGTCTGTAC CTTAAAAATG GAGGTCATTT CATTGTGTTC 1101 ATTTTCAAGG TTGTTGTATG GCTCGGTCAG AACTTTCTGT TACCAGAAGA 1151 CACTCACATT CAGAATGCTC CATTTCAAGT GTGTTTCACA TCTTTACGGA 1201 ATGGCGGCCA CCTGCATATA AAAATAAAAC TTAGTGGAGA GATCACTATA 1251 AATACTGATG ATATTGATTT GGCTGGTGAT ATCATCCAGT CAATGGCATC 1301 ATTTTTTGCT ATTGAAGACC TTCAAGTAGA AGCGGATTTT CCTGTCTATT 1351 TTGAGGAATT ACGAAAGGTG CTAGTTAAGG TGGATGAATA TCATTCAGTG 1401 CATCAGAAGC TCAGTGCTGA TATGGCTGAT CATTCTAATT TGATCCGAAG 1451 TTTGCTGGTC GGAGCTGAGG ATGCTCGTCT GATGAGGGAC ATGAAAACAA 1501 TGAAGAGTCG TTATATGGAA CTCTATGACC TTAATAGAGA CTTGCTAAAT 1551 GGATATAAAA TTCGCTGTAA CAATCACACA GAGCTGTTGG GAAACCTCAA 1601 AGCAGTAAAT CAAGCAATTC AAAGAGCAGG TCGTCTGCGG GTTGGAAAAC 1651 CAAAGAACCA GGTGATCACT GCTTGTCGGG ATGCAATTCG AAGCAATAAC 1701 ATCAACACAC TGTTCAAAAT CATGCGAGTG GGGACAGCTT CTTCCTAGGT 1751 GAGGAAAATA CAGGTCATGA AGTTCCTGGC AAAGATTTTC TGTTAAAAAC 1801 CTATGCTGGT TTGCTTTGGA TCACACCCTG GTGAACCCCG GGTGCTAAGA 1851 ATGAAAATAA CCTTGGTGAG TTGTACAAAT TAAAGACAAA GAACTACATG 1901 TGAAGATAGA CTTGCTTTCT ATTTTTAAAT CAGTAGTAGT ACTGTTGCTG 1951 AATAATACTA GGTTTTTATG GAATAGGATG AATGCTTTTG AAGTATTAGG 2001 GCTTCAGAGT CCAATTTTGC TTATTTATGG TATATAAATA CATATTTTTT 2051 TCTTGAAATT GCAATTGAGT TTGTACTTTT CAAATAGATT ATCTACTTTT 2101 TCATTAAAAT GTAAAGATGT TAAAAAAAAA AAAAAAAAAA AA BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 3 ORF from 1293 bp to 1745 bp; peptide length: 151 Category: similarity to unknown protein Classification: no clue 1 MASFFAIEDL QVEADFPVYF EELRKVLVKV DEYHSVHQKL SADMADHSNL 51 IRSLLVGAED ARLMRDMKTM KSRYMELYDL NRDLLNGYKI RCNNHTELLG 101 NLKAVNQAIQ RAGRLRVGKP KNQVITACRD AIRSNNINTL FKIMRVGTAS 151 S BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_2ι5, frame 3 TREMBL :CEF20D12_1 gene: "F20D12.3"; Caenorhabditis elegans cosmid F20D12., N = 1, Score = 173, P = 4.5e-12 >TREMBL:CEF20D12_1 gene: "F20D12.3"; Caenorhabditis elegans cosmid F20D12. Length = 699 HSPs: Score = 173 (26.0 bits), Expect = 4.5e-12, P = 4.5e-12 Identities = 33/130 (25%), Positives = 72/130 (55%) Query: 20 FEELRKVLVKVDEYHSVHQKLSADMADHSNLIRSLLVGAEDARLMRDMKTMKSRYMELYD 79 F+E ++L ++D V +L+A++ + ++ +++ AED+ + ++ + Y+ L Sbjct: 569 FKEADEILEEIDPMTEVRDRLTAELQERQAAVKEIIIRAEDSIAIDNIPDARKFYIRLKA 628 Query: 80 LNRDLLNGYKIRCNNHTELLGNLKAVNQAIQRAGRLRVGKPKNQVITACRDAIRSNNINT 139 + ++R NN + +L+ +N+ 1+ RLRVG+P Q++ +CR Al +N Sbjct: 629 NDAAARQAAQLRWNNQERCVKΞLRRLNKIIENCSRLRVGEPGRQIVVSCRSAIADDNKQI 688 Query: 140 LFKIMRVGTA 149 + KI++ G + Sbjct: 689 ITKILQYGAS 698 Pedant information for DKFZphtes3_2ι5, frame 3 Report for DKFZphtes3_2ι5.3 [LENGTH] 151 [MW] 17304.07 [pi] 9.33 [HOMOL] TREMBL :CEF20D12_1 gene: "F20D12.3"; Caenorhabditis elegans cosmid F20D12. 2e-12 [KW] Alpha_Beta SEQ MASFFAIEDLQVEADFPVYFEELRKVLVKVDEYHSVHQKLSADMADHSNLIRSLLVGAED PRD ccceeeehhhhhhccccchhhhhhhhhhhhccchhhhhhhhhhhhhhhhhhhhhhhhhhh SEQ ARLMRDMKTMKΞRYMELYDLNRDLLNGYKIRCNNHTELLGNLKAVNQAIQRAGRLRVGKP PRD hhhhhccchhhhhheeeccchhhhhhheeeeeccchhhhhhhhhhhhhhhhhcccccccc SEQ KNQVITACRDAIRSNNINTLFKIMRVGTASS PRD cceeeeehhhhhhcccceeeeccceeecccc (No Prosite data available for DKFZphtes3_2ι5.3) (No Pfam data available for DKFZphtes3_2ι5.3) DKFZphtes3_2119 group: testes derived DKFZphtes3_2119 encodes a novel 166 amino acid protein without similarity to known proteins. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . unknown complete cDNA, complete eds, no EST hits Sequenced by EMBL Locus : unknown Insert length: 1079 bp Poly A stretch at pos. 1053, polyadenylation signal at pos. 1038 1 CCACAGGACA CACTGTTCCC AGGGCACAGA CACCCTGGGC TTTGGTTGGG 51 TCTTGGCCTC CAGGTAGGGC CCTGTTGGGC AGCGGGCAGC AACTCCTGAG 101 ACACTACTGT GATTCTTGGT GGTGGCTGTG GTAAAAAACC TGCAGGGCTA 151 GAGTTTGGGG TGAGATTCAG CAGTAACTGT GGCCTCTCCT AGTGACAGTA 201 TGTCACTCCC ACTCCCAGCA CGCATGCCCA CAGGCCACGG CCTCCACATC 251 ACAAACCCCC CACCAAGTTG CCCATCTATG GAGCAGCTCC CATACGGCAG 301 GGTCAGGCTC TTACCTCCAC CTCCAGGGCA CAGACAGGGG GAGCTCTGTC 351 TCACTGTAAG GCAATGAGGA GAGTTGAGGG CCCAGACCAG GCTAGGGGCC 401 ATCCCCTTTC CCGAGCAGGC CTCAGGGAAG GACCAGCCCC ATTCCCATCT 451 GACCTAGGTC TTAGCCCAGG AGCCTGCATA GGGAAGAAAG GACAGACAGG 501 GCCTCCTTAC TGGCTGACAC TCAGGAGGGG CTGGGGCAAG AGAGCAGAGG 551 GAGCGCAGGG CCAGGCAGGG GCTGCTGAGG ATCCATGGGA GCTCAGGGTG 601 CACAAGGGGG CTGCCCTTCC TGGGCTGCAG GCAGCATCCC TATGGGAGCT 651 GAGAAAGTCC AATCCTGAGA TGGGACAGTG CTGCCCAGGG GTGTGTGGCT 701 GGGCCCTGAC AACAGTCTCC CCAAAAGTGA CCACATCACC AGGCTCAGTT 751 CCAGGAAGGC TGAGAAGTGC CCAGTACACT GAGGATGCAC CTCAGTTACA 801 TAAAATAAAT GAAACTGGAG TACTAACGTA CAGTTTAAAG GTTATAGTTA 851 CTATTTTTAT ATGATATACT AGTAATTTTT GAATAGGGTA AACTTTAGGT 901 GTTTTGACAC CAAAAGAAAA CTACATGAGT TCATGCATGT GTTAAATTGC 951 TTTACTGTAG TAATCATTTA CATGTATATG TATATATGAA TATAATTATG 1001 GGCTCATTAA ATTTAAATAT TATAAATAGG TGACAAAGAA TAAAGTTAAC 1051 TGGAAAAAAA AAAAAAAAAA AAAAAAAAA BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 1 ORF from 364 bp to 861 bp; peptide length: 166 Category: putative protein Classification: no clue 1 MRRVEGPDQA RGHPLSRAGL REGPAPFPSD LGLSPGACIG KKGQTGPPYW 51 LTLRRGWGKR AEGAQGQAGA AEDPWELRVH KGAALPGLQA ASLWELRKSN 101 PEMGQCCPGV CGWALTTVSP KVTTSPGSVP GRLRSAQYTE DAPQLHKINE 151 TGVLTYSLKV IVTIFI BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_2119, frame 1 No Alert BLASTP hits found Pedant information for DKFZphtes3_2119, frame 1 Report for DKFZphtes3_2119.1 [LENGTH] 166 [MW] 17691.35 [pi] 9.54 [KW] All Beta [KW] LOW COMPLEXITY 7.23 % SEQ MRRVEGPDQARGHPLSRAGLREGPAPFPSDLGLSPGACIGKKGQTGPPYWLTLRRGWGKR SEG PRD ccccccccccccccccccccccccccccccccccccceeeccccccccceeeeecccccc SEQ AEGAQGQAGAAEDPWELRVHKGAALPGLQAASLWELRKSNPEMGQCCPGVCGWALTTVSP SEG xxxxxxxxxxxx PRD ccccccccccccccceeeeccccccccchhhhhhhhhhcccccccccccccceeeeeccc SEQ KVTTSPGSVPGRLRSAQYTEDAPQLHKINETGVLTYSLKVIVTIFI SEG PRD ccccccccccccccccccccccccceeeccccceeeehhhhhhccc (No Prosite data available for DKFZphtes3_2119.1) (No Pfam data available for DKFZphtes3_2119.1) DKFZphtes3_2ml8 group: nucleic acid management DKFZphtes3_2ml8 encodes a novel amino acid protein, with similarity to mouse Dhml . The protein seems to play a role in nucleotide metabolism, RNA metabolism, but also in DNA repair and cell cycle. The yeast homologue is a DNA strand exchange protein required for sporulation and homologous recombination. The novel protein can find application as multifunctional nuclease / exoribonuclease. nearly identical to mouse Dhml complete cDNA, complete eds, start at Bp 42, EST hits Sequenced by EMBL Locus: unknown Insert length: 3022 bp Poly A stretch at pos. 3004, polyadenylation signal at pos. 2981 1 CTCGTCAGCC GGTCGGCCGC CGCCTCCAGC CGTGTGCCGC TATGGGAGTC 51 CCGGCGTTCT TCCGCTGGCT CAGCCGCAAG TACCCGTCCA TCATAGTCAA 101 CTGCGTGGAA GAGAAGCCAA AAGAATGCAA TGGTGTAAAG ATTCCAGTTG 151 ATGCCAGTAA ACCTAATCCA AATGATGTGG AGTTTGATAA TCTGTATTTG 201 GATATGAATG GAATCATCCA TCCCTGTACT CATCCTGAAG ACAAACCAGC 251 ACCAAAAAAT GAAGATGAAA TGATGGTTGC AATTTTTGAG TACATTGACA 301 GACTTTTCAG TATTGTAAGA CCAAGAAGAC TTCTCTACAT GGCAATAGAT 351 GGAGTGGCAC CACGTGCTAA AATGAACCAG CAGCGTTCAA GGAGGTTCAG 401 GGCATCAAAA GAAGGAATGG AAGCAGCAGT CGAGAAGCAG CGAGTCAGGG 451 AAGAAATATT GGCAAAAGGT GGCTTTCTTC CTCCAGAAGA AATAAAAGAA 501 AGATTTGACA GCAACTGTAT TACACCAGGA ACTGAATTCA TGGACAATCT 551 TGCTAAATGC CTTCGCTATT ACATAGCTGA TCGTTTAAAT AATGACCCTG 601 GGTGGAAAAA TTTGACAGTT ATTTTATCTG ATGCTAGTGC TCCTGGTGAA 651 GGAGAACATA AAATCATGGA TTACATTAGA AGGCAAAGAG CCCAGCCTAA 701 CCATGACCCA AATACTCATC ATTGTTTATG TGGAGCAGAT GCTGATCTCA 751 TTATGCTTGG CCTTGCCACA CATGAACCGA ACTTTACCAT TATTAGAGAA 801 GAATTCAAAC CAAACAAGCC CAAACCATGT GGTCTTTGTA ATCAGTTTGG 851 ACATGAGGTC AAAGATTGTG AAGGTTTGCC AAGAGAAAAG AAGGGAAAGC 901 ATGATGAACT TGCCGATAGT CTTCCTTGTG CAGAAGGAGA GTTTATCTTC 951 CTTCGGCTTA ATGTTCTTCG TGAGTATTTG GAAAGAGAAC TCACAATGGC 1001 CAGCCTACCA TTCACATTTG ATGTTGAGAG GAGCATTGAT GACTGGGTTT 1051 TCATGTGCTT CTTTGTGGGA AATGACTTCC TCCCTCATTT GCCATCGTTA 1101 GAGATTAGGG AAAATGCAAT TGACCGTTTG GTTAACATAT ACAAAAATGT 1151 GGTACACAAA ACTGGGGGTT ACCTTACAGA AAGTGGTTAT GTCAATCTGC 1201 AAAGAGTACA GATGATCATG TTAGCAGTTG GTGAAGTTGA GGATAGCATT 1251 TTTAAAAAGA GAAAGGATGA TGAGGACAGT TTTAGAAGAC GACAGAAAGA 1301 AAAAAGAAAG AGAATGAAGA GAGATCAACC AGCTTTCACT CCTAGTGGAA 1351 TATTAACTCC TCATGCCTTG GGTTCAAGAA ATTCACCAGG TTCTCAAGTA 1401 GCCAGTAATC CGAGACAAGC AGCCTATGAA ATGAGGATGC AGAATAACTC 1451 TAGTCCTTCG ATATCTCCTA ATACGAGTTT CACATCTGAT GGCTCCCCGT 1501 CTCCATTAGG AGGAATTAAG CGAAAAGCAG AAGACAGTGA CAGTGAACCT 1551 GAGCCAGAGG ATAATGTCAG GTTATGGGAA GCTGGCTGGA AGCAGCGGTA 1601 CTACAAGAAC AAATTTGATG TGGATGCAGC TGATGAGAAA TTCCGTCGGA 1651 AAGTTGTGCA GTCGTACGTT GAAGGACTTT GCTGGGTTCT TAGATATTAT 1701 TACCAGGGCT GTGCTTCCTG GAAGTGGTAT TATCCATTTC ATTATGCACC 1751 ATTTGCTTCA GACTTTGAAG GCATTGCAGA CATGCCATCT GATTTTGAGA 1801 AGGGTACGAA ACCGTTTAAA CCACTAGAAC AACTTATGGG GGTATTTCCA 1851 GCTGCAAGTG GTAATTTTCT ACCTCCATCA TGGCGGAAGC TCATGAGTGA 1901 TCCTGATTCT AGTATAATTG ACTTCTATCC TGAAGATTTT GCTATTGATT 1951 TGAATGGGAA GAAATATGCA TGGCAAGGTG TTGCTCTCTT GCCATTCGTG 2001 GATGAGCGAA GGCTACGAGC TGCCCTAGAA GAGGTATACC CAGACCTCAC 2051 TCCAGAAGAG ACCAGAAGAA ACAGCCTTGG AGGTGATGTC TTATTTGTGG 2101 GGAAACATCA CCCACTCCAT GACTTCATTT TAGAGCTGTA CCAGACAGGT 2151 TCCACAGAGC CAGTGGAGGT ACCCCCTGAA CTATGTCATG GGATTCAAGG 2201 AAAGTTTTCT TTGGATGAAG AAGCCATTCT TCCAGATCAA ATAGTATGTT 2251 CTCCTGTTCC TATGTTAAGG GATCTGACAC AGAACACTGT AGTCAGTATT 2301 AATTTTAAAG ACCCACAGTT TGCTGAAGAT TACATTTTTA AAGCTGTAAT 2351 GCTTCCAGGA GCAAGAAAGC CAGCAGCAGT ACTGAAACCT AGTGACTGGG 2401 AAAAATCCAG CAATGGACGG CAGTGGAAGC CTCAGCTTGG CTTTAACCGT 2451 GACCGGAGGC CTGTGCACCT GGATCAGGCA GCCTTCAGGA CTTTGGGCCA 2501 TGTGATGCCA AGAGGCTCAG GAACTGGCAT TTACAGCAAT GCTGCACCAC 2551 CACCTGTGAC TTACCAGGGA AACTTATACA GGCCGCTTTT GAGAGGACAA 2601 GCCCAGATTC CAAAACTTAT GTCAAATATG AGGCCCCAGG ATTCCTGGCG 2651 AGGTCCTCCT CCCCTTTTCC AGCAGCAAAG GTTTGACAGA GGCGTTGGGG 2701 CTGAACCTCT GCTCCCATGG AACCGGATGC TGCAAACCCA GAATGCAGCC 2751 TTCCAGCCAA ACCAGTACCA GATGCTAGCT GGGCCTGGTG GGTATCCACC 2801 CAGACGAGAT GATCGTGGAG GGAGACAGGG ATATCCCAGA GAAGGAAGGA 2851 AATACCCTTT GCCACCACCC TCAGGAAGAT ACAATTGGAA TTAAGCTTTT 2901 GTAAAGCTTT CCCAAATCCT TTCATCATTC TACAGTTTTA TGCTATTTGT 2951 GGAAAGATTT CCTTCTCAAG TAGTAGTTTT TAATAAAACT ACAGTACTTT 3001 GTGTAAAAAA AAAAAAAAAA AA BLAST Results No BLAST result Medline entries 95192042: Characterization of cDNA encoding mouse homolog of fission yeast dhpl÷ gene: structural and functional conservation. 97361754: Cloning and characterization of mouse Dhm2 cDNA, a functional homolog of budding yeast SEP1. Peptide information for frame 3 ORF from 42 bp to 2891 bp; peptide length: 950 Category: strong similarity to known protein 1 MGVPAFFRWL SRKYPSIIVN CVEEKPKECN GVKIPVDASK PNPNDVEFDN 51 LYLDMNGIIH PCTHPEDKPA PKNEDEMMVA IFEYIDRLFS IVRPRRLLYM 101 AIDGVAPRAK MNQQRSRRFR ASKEGMEAAV EKQRVREEIL AKGGFLPPEE 151 IKERFDSNCI TPGTEFMDNL AKCLRYYIAD RLNNDPGWKN LTVILSDASA 201 PGEGEHKIMD YIRRQRAQPN HDPNTHHCLC GADADLIMLG LATHEPNFTI 251 IREEFKPNKP KPCGLCNQFG HEVKDCEGLP REKKGKHDEL ADSLPCAEGE 301 FIFLRLNVLR EYLERELTMA SLPFTFDVER ΞIDDWVFMCF FVGNDFLPHL 351 PSLEIRENAI DRLVNIYKNV VHKTGGYLTE SGYVNLQRVQ MIMLAVGEVE 401 DSIFKKRKDD EDSFRRRQKE KRKRMKRDQP AFTPSGILTP HALGSRNSPG 451 SQVASNPRQA AYEMRMQNNS SPSISPNTSF TSDGSPSPLG GIKRKAEDSD 501 SEPEPEDNVR LWEAGWKQRY YKNKFDVDAA DEKFRRKVVQ SYVEGLCWVL 551 RYYYQGCASW KWYYPFHYAP FASDFEGIAD MPSDFEKGTK PFKPLEQLMG 601 VFPAASGNFL PPΞWRKLMSD PDSΞIIDFYP EDFAIDLNGK KYAWQGVALL 651 PFVDERRLRA ALEEVYPDLT PEETRRNΞLG GDVLFVGKHH PLHDFILELY 701 QTGSTEPVEV PPELCHGIQG KFSLDEEAIL PDQIVCSPVP MLRDLTQNTV 751 VSINFKDPQF AEDYIFKAVM LPGARKPAAV LKPSDWEKΞS NGRQWKPQLG 801 FNRDRRPVHL DQAAFRTLGH VMPRGSGTGI YSNAAPPPVT YQGNLYRPLL 851 RGQAQIPKLM SNMRPQDSWR GPPPLFQQQR FDRGVGAEPL LPWNRMLQTQ 901 NAAFQPNQYQ MLAGPGGYPP RRDDRGGRQG YPREGRKYPL PPPSGRYNWN BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_2ml8, frame 3 PIR: 149635 mouse Dhml protein - mouse, N = 1, Score = 4765, P = 0 PIR:S43891 dhpl protein - fission yeast (Schizosaccharomyces pombe), N = 3, Score = 1172, P = 2e-197 PIR:S20126 exoribonuclease RAT1 (EC 3.1.11.-) - yeast (Saccharomyces cerevisiae), N = 2, Score = 1146, P = 3.8e-175 PIR:S72531 exonuclease II - fission yeast (Schizosaccharomyces pombe), N = 4, Score = 622, P = 4.2e-125 >PIR: 149635 mouse Dhml protein Length = 947 HSPs: Score = 4765 (714.9 bits), Expect = O.Oe+00, P = O.Oe+00 Identities = 884/930 (95%), Positives = 895/930 (96%) Query: 1 MGVPAFFRWLSRKYPSIIVNCVEEKPKECNGVKIPVDASKPNPNDVEFDNLYLDMNGIIH 60 MGVPAFFRWLSRKYPSIIVNCVEEKPKECNGVKIPVDASKPNPNDVEFDNLYLDMNGIIH Sb j ct: 1 MGVPAFFRWLSRKYPSIIVNCVEEKPKECNGVKIPVDASKPNPNDVEFDNLYLDMNGIIH 60 Query: 61 PCTHPEDKPAPKNEDEMMVAIFEYIDRLFSIVRPRRLLYMAIDGVAPRAKMNQQRSRRFR 120 PCTHPEDKPAPKNEDEMMVAIFEYIDRLF+IVRPRRLLYMAIDGVAPRAKMNQQRSRRFR Sbjct: 61 PCTHPEDKPAPKNEDEMMVAIFEYIDRLFNIVRPRRLLYMAIDGVAPRAKMNQQRSRRFR 120 Query: 121 ASKEGMEAAVEKQRVREEILAKGGFLPPEEIKERFDSNCITPGTEFMDNLAKCLRYYIAD 180 A K GMEAAVEKQRVREEILAKGGFLPPEEIKERFDSNCITPGTEFMDNLAKCLRYYIAD Sbjct: 121 AIKGGMEAAVEKQRVREEILAKGGFLPPEEIKERFDSNCITPGTEFMDNLAKCLRYYIAD 180 Query: 181 RLNNDPGWKNLTVILSDASAPGEGEHKIMDYIRRQRAQPNHDPNTHHCLCGADADLIMLG 240 RLNNDPGWKNLTVILSDASAPGEGEHKIMDYIRRQRAQPN DPNTHHCLCGADADLIMLG Sbjct: 181 RLNNDPGWKNLTVILSDASAPGEGEHKIMDYIRRQRAQPNQDPNTHHCLCGADADLIMLG 240 Query: 241 LATHEPNFTIIREEFKPNKPKPCGLCNQFGHEVKDCEGLPREKKGKHDELADSLPCAEGE 300 LATHEPNFTIIREEFKPNKPKPC LCNQFGHEVKDCEGLPREKKGKHDELADSLPCAEGE Sbjct: 241 LATHEPNFTIIREEFKPNKPKPCALCNQFGHEVKDCEGLPREKKGKHDELADΞLPCAEGE 300 Query: 301 FIFLRLNVLREYLERELTMASLPFTFDVERSIDDWVFMCFFVGNDFLPHLPSLEIRENAI 360 FIFLRLNVLREYLERELTMASLPF FDVERS DDW FMCFFVGNDFLPHLPSLEIRE Al Sbjct: 301 FIFLRLNVLREYLERELTMASLPFPFDVERSNDDWEFMCFFVGNDFLPHLPSLEIREGAI 360 Query: 361 DRLVNIYKNVVHKTGGYLTESGYVNLQRVQMIMLAVGEVEDSIFKKRKDDEDSFRRRQKE 420 DRLVNIYKNVVHKTGGYLTESGYVNLQRVQMIMLAVGEVEDSIFKKRKDDEDSFRRRQKE Sbjct: 361 DRLVNIYKNVVHKTGGYLTESGYVNLQRVQMIMLAVGEVEDΞIFKKRKDDEDSFRRRQKE 420 Query: 421 KRKRMKRDQPAFTPSGILTPHALGSRNSPGSQVAΞNPRQAAYEMRMQNNSSPSISPNTSF 480 KRKRMKRDQPAFTPSGILTPHALGSRNSPG QVASNPRQAAYEMRMQ NSSPSISPNTSF Sbjct: 421 KRKRMKRDQPAFTPSGILTPHALGSRNSPGCQVASNPRQAAYEMRMQRNSSPSISPNTSF 480 Query: 481 TSDGSPSPLGGIKRKAEDSDSEPEPEDNVRLWEAGWKQRYYKNKFDVDAADEKFRRKVVQ 540 SDGSPSPLGGI+RKAEDSDSEPEPEDNVRLWEAGWKQRYYKNKFDVDAADEKFRRKVVQ Sbjct: 481 ASDGSPSPLGGIRRKAEDSDSEPEPEDNVRLWEAGWKQRYYKNKFDVDAADEKFRRKVVQ 540 Query: 541 SYVEGLCWVLRYYYQGCASWKWYYPFHYAPFASDFEGIADMPSDFEKGTKPFKPLEQLMG 600 SYVEGLCWVLRYYYQGCASWKW YPFHYAPFASDFEGIADM S+FEKGTKPFKPLEQLMG Sbjct: 541 SYVEGLCWVLRYYYQGCASWKWLYPFHYAPFASDFEGIADMSSEFEKGTKPFKPLEQLMG 600 Query: 601 VFPAASGNFLPPSWRKLMSDPDSSIIDFYPEDFAIDLNGKKYAWQGVALLPFVDERRLRA 660 VFPAASGNFLPP+WRKLMSDPDSSIIDFYPEDFAIDLNGKKYAWQGVALLPFVDERRLRA Sbjct: 601 VFPAASGNFLPPTWRKLMSDPDSSIIDFYPEDFAIDLNGKKYAWQGVALLPFVDERRLRA 660 Query: 661 ALEEVYPDLTPEETRRNSLGGDVLFVGKHHPLHDFILELYQTGSTEPVEVPPELCHGIQG 720 ALEEVYPDLTPEE RRNSLGGDVLFVGK HPL DFILELYQTGSTEPV+VPPELCHGIQG Sbjct: 661 ALEEVYPDLTPEENRRNSLGGDVLFVGKLHPLRDFILELYQTGSTEPVDVPPELCHGIQG 720 Query: 721 KFSLDEEAILPDQIVCSPVPMLRDLTQNTVVSINFKDPQFAEDYIFKAVMLPGARKPAAV 780 FSLDEEAILPDQ VCSPVPMLRDLTQNT VSINFKDPQFAEDY+FKA MLPGARKPA V Sbjct: 721 TFSLDEEAILPDQTVCSPVPMLRDLTQNTAVSINFKDPQFAEDYVFKAAMLPGARKPATV 780 Query: 781 LKPSDWEKSSNGRQWKPQLGFNRDRRPVHLDQAAFRTLGHVMPRGSGTGIYSNAAPPPVT 840 LKP DWEKSSNGRQWKPQLGFNRDRRPVHLDQAAFRTLGHV PRGSGT +Y+N A P Sbjct: 781 LKPGDWEKSSNGRQWKPQLGFNRDRRPVHLDQAAFRTLGHVTPRGSGTSVYTNTALLPAN 840 Query: 841 YQGNLYRPLLRGQAQIPKLMSNMRPQDSWRGPPPLFQQQRFDRGVGAEPLLPWNRMLQTQ 900 YQGN YRPLLRGQAQIPKLMSNMRP+DSWRGPPPLFQQ RF+R VGAEPLLPWNRM+Q Q Sbjct: 841 YQGNNYRPLLRGQAQIPKLMSNMRPKDΞWRGPPPLFQQHRFERSVGAEPLLPWNRMIQNQ 900 Query: 901 NAAFQPNQYQMLAGPGGYPPRRDD-RGGRQ 929 NAAFQPNQYQML GPGGYPPRRDD RGGRQ Sbjct: 901 NAAFQPNQYQMLGGPGGYPPRRDDHRGGRQ 930 Pedant information for DKFZphtes3_2ml8, frame 3 Report for DKFZphtes3_2ml8.3 [LENGTH] 950 [MW] 108582.68 [pl] 7.26 [HOMOL] PIR: 149635 mouse Dhml protein - mouse 0.0 [ FUNCAT] 08.01 nuclear transport [S. cerevisiae, YOR048C] le-123 [FUNCAT] 04.01.04 rrna processing [S. cerevisiae, YOR048c] le-123 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YOR048c] le-123 [FUNCAT] 01.03.16 polynucleotide degradation [S. cerevisiae, YGL173c] 3e- 79 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YGL173c] 3e- 79 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YGL173c] 3e- 79 [PIRKW] nucleus le-126 [PIRKW] hydrolase le-122 [PIRKW] exoribonuclease le-122 [PROSITE] MYRISTYL 7 [PROSITE] AMIDATION 2 [PROSITE] CAMP_PHOSPHO_SITE 1 [PROSITE] CK2_PHOSPHO_SITE 12 [PROSITE] TYR_PHOSPHO_SITE 1 [PROSITE] GLYCOSAMINOGLYCAN 1 [PROSITE] PKC_PHOSPHO_SITE 8 [PROSITE] ASN_GLYCOSYLATION 4 [KW] TRANSMEMBRANE 1 [KW] LOW COMPLEXITY 6.21 % SEQ MGVPAFFRWLSRKYPSIIVNCVEEKPKECNGVKIPVDASKPNPNDVEFDNLYLDMNGIIH SEG PRD cccchhhhhhhhhcceeeeeeecccccccccccccccccccccccccccceeeeccceee MEM SEQ PCTHPEDKPAPKNEDEMMVAIFEYIDRLFSIVRPRRLLYMAIDGVAPRAKMNQQRSRRFR SEG PRD ccccccccccccchhhhhhhhhhhhhhhhhhhhcceeeeeeeccccchhhhhhhhhhhhh MEM SEQ ASKEGMEAAVEKQRVREEILAKGGFLPPEEIKERFDSNCITPGTEFMDNLAKCLRYYIAD SEG PRD hhhhhhhhhhhhhhhhhhhhhhcccccccccccccccccccccchhhhhhhhhhhhhhhh MEM SEQ RLNNDPGWKNLTVILSDASAPGEGEHKIMDYIRRQRAQPNHDPNTHHCLCGADADLIMLG SEG PRD hcccccccceeeeeeeccccccccchhhhhhhhhhhhccccccccccccccccccceeec MEM SEQ LATHEPNFTIIREEFKPNKPKPCGLCNQFGHEVKDCEGLPREKKGKHDELADSLPCAEGE SEG PRD ccccccccccccccccccccccceeeccccccccccccccchhhhhhhhhcccccccccc MEM SEQ FIFLRLNVLREYLERELTMASLPFTFDVERSIDDWVFMCFFVGNDFLPHLPSLEIRENAI SEG PRD ccchhhhhhhhhhhhhhhhhhhchhhhhhhhhhhheeeeeeccccccccccccccchhhh MEM MMMMMMMMMMMMMMMMMMM SEQ DRLVNIYKNVVHKTGGYLTESGYVNLQRVQMIMLAVGEVEDSIFKKRKDDEDSFRRRQKE SEG xxxxxx PRD hhhhhhhhhhhcccccccccccchhhhhhhhhhhhhhcchhhhhhhhhhhhhhhhhhhhh MEM SEQ KRKRMKRDQPAFTPΞGILTPHALGSRNSPGSQVASNPRQAAYEMRMQNNSSPΞISPNTSF SEG xxxxxxx xxxxxxxxxxxxx PRD hhhhhhhhcccccccccccccccccccccccchhhhhhhhhhhhhhhccccccccccccc MEM SEQ TSDGSPSPLGGIKRKAEDSDSEPEPEDNVRLWEAGWKQRYYKNKFDVDAADEKFRRKVVQ SEG XX xxxxxxxxxxx PRD ccccccccchhhhhhhhcccccccchhhhhhhhhhhhhhhhhhhhcccchhhhhhhhhhh MEM SEQ SYVEGLCWVLRYYYQGCASWKWYYPFHYAPFASDFEGIADMPSDFEKGTKPFKPLEQLMG SEG PRD hhhhhhheeeeeeccccccccccccccccccccccccccccccccccccccccchhhhhh MEM SEQ VFPAASGNFLPPSWRKLMSDPDSSIIDFYPEDFAIDLNGKKYAWQGVALLPFVDERRLRA SEG PRD hccccccccccccccccccccccceeeccccceeeccccccceeeeeeeeeccchhhhhh MEM SEQ ALEEVYPDLTPEETRRNSLGGDVLFVGKHHPLHDFILELYQTGΞTEPVEVPPELCHGIQG SEG PRD hhhhhccccchhhhhhcccccceeeeeecccchhhhhhhhhcccccceeecccccccccc MEM SEQ KFSLDEEAILPDQIVCSPVPMLRDLTQNTVVSINFKDPQFAEDYIFKAVMLPGARKPAAV SEG PRD cccccceeecccceeeccccccccccccceeeeecccccchhhhheeeccccccccccee MEM SEQ LKPSDWEKSSNGRQWKPQLGFNRDRRPVHLDQAAFRTLGHVMPRGSGTGIYSNAAPPPVT SEG PRD eccccccccccccccccccccccccccccchhhhhhhhhhcccccccccccccccccccc MEM SEQ YQGNLYRPLLRGQAQIPKLMSNMRPQDSWRGPPPLFQQQRFDRGVGAEPLLPWNRMLQTQ SEG PRD cccccchhhhhcccchhhhhcccccccccccccccchhhhhccccccccccccchhhhhh MEM SEQ NAAFQPNQYQMLAGPGGYPPRRDDRGGRQGYPREGRKYPLPPPSGRYNWN SEG xxxxxxxxxxxxxxxxxxxx PRD hcccccccceeecccccccccccccccccccccccccccccccccccccc MEM Prosite for DKFZphtes3_2ml8.3 PS00001 190->194 ASN_GLYCOSYLATION PDOC00001 PS00001 247->251 ASN_GLYCOSYLATION PDOC00001 PΞ00001 468->472 ASN_GLYCOSYLATION PDOC00001 PS00001 477->481 ASN_GLYCOSYLATION PDOC00001 PS00002 826->830 GLYCOSAMINOGLYCAN PDOC00002 PS00004 675->679 CAMP_PHOSPHO_SITE PDOC00004 PS00005 11->14 PKC_PHOSPHO_SITE PDOC00005 PS00005 116->119 PKC_PHOSPHO_SITE PDOC00005 PS00005 413->416 PKC_PHOSPHO_SITE PDOC00005 PS00005 559->562 PKC_PHOSPHO_SITE PDOC00005 PS00005 613->616 PKC_PHOSPHO_SITE PDOC00005 PS00005 674->677 PKC_PHOSPHO_SITE PDOC00005 PS00005 868->871 PKC_PHOSPHO_SITE PDOC00005 PS00005 944->947 PKC_PHOSPHO_SITE PDOC00005 PS00006 63->67 CK2_PHOSPHO_SITE PDOC00006 PS00006 331->335 CK2_PHOSPHO_ΞITE PDOC00006 PS00006 499->503 CK2_PHOSPHO_SITE PDOC00006 PS00006 501->505 CK2_PHOSPHO_SITE PDOC00006 PS00006 541->545 CK2_PHOSPHO_SITE PDOC00006 PS00006 573->577 CK2_PHOSPHO_SITE PDOC00006 PS00006 583->587 CK2_PHOSPHO_SITE PDOC00006 PS00006 619->623 CK2_PHOSPHO_SITE PDOC00006 PS00006 624->628 CK2_PHOSPHO_SITE PDOC00006 PS00006 670->674 CK2_PHOSPHO_SITE PDOC00006 PS00006 723->727 CK2_PHOSPHO_SITE PDOC00006 PS00006 784->788 CK2_PHOSPHO_SITE PDOC00006 PS00007 659->667 TYR_PHOSPHO_SITE PDOC00007 PS00008 125->131 MYRISTYL PDOC00008 PS00008 375->381 MYRISTYL PDOC00008 PS00008 450->456 MYRISTYL PDOC00008 PS00008 600->606 MYRISTYL PDOC00008 PS00008 825->831 MYRISTYL PDOC00008 PS00008 829->835 MYRISTYL PDOC00008 PS00008 926->932 MYRISTYL PDOC00008 PS00009 638->642 AMIDATION PDOC00009 PS00009 934->938 AMIDATION PDOC00009 (No Pfam data available for DKFZphtes3 2ml8.3) DKFZphtes3_2m20 group: testes derived DKFZphtes3_2m20 encodes a novel 183 amino acid protein without similarity to known proteins. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . group: unknown DKFZphtes3_2m20 encodes a novel ammo acid protein without similarity to known proteins. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . unknown EST hits are only from testis or uterus librarys remaining intron m3 ' UTR see EST-BLAST Sequenced by EMBL Locus: unknown Insert length: 1341 bp Poly A stretch at pos. 1320, polyadenylation signal at pos. 1300 1 GCAATCCAGG AGCTGAATGG TAACTCTTCC ACAAGCGAAA ACTGTTCGTG 51 AATACAAGCA AAAGGCCCCC CAAGAGGACC CCTGATATGA TCCAGCAGCC 101 TCGGGCCCCG CTGGTGTTGG AGAAGGCTTC TGGTGAAGGA TTTGGCAAAA 151 CCGCCGCTAT TATACAGCTC GCTCCTAAAG CTCCTGTTGA CCTGTGTGAG 201 ACAGAGAAAC TGAGGGCAGC CTTCTTTGCA GTCCCGTTGG AAATGAGAGG 251 GTCCTTCCTG GTGCTGCTCC TGAGGGAATG CTTCCGAGAC CTGAGCTGGC 301 TGGCACTCAT CCATAGCGTC CGTGGGGAGG CGGGGCTGCT GGTGACGAGT 351 ATCGTCCCGA AGACCCCGTT TTTCTGGGCC ATGCACATCA CTGAGGCTCT 401 GCACCAGAAC ATGCAGGCTC TGTTTAGCAC CCTGGCTCAG GCGGAGGAGC 451 AGCAGCCCTA CCTGGAGGCT CCACCGTTAT GCGCGGGACT CGCTGTCTGG 501 CAGAGTACCA CCTGGGGGAT TATGGACACG CCTGGAACAG GTGTTGGGTG 551 CTGGACAGGG TGGACACCTG GGCTGTGGTC ATGTTCATTG ATTTTGGACA 601 GTTGGCCACC ATCCCTGTGC AGTCTCTGCG CCAGCTAGAC AGCGACGACT 651 TCTGGACCAT CCCACCCCTG ACTCAGCCAT TCATGCTGGA GAAAGACATT 701 TTGAGTTCGT ATGAGGTTGT CCATCGAATC CTCAAAGGGA AAATCACTGG 751 TGCTTTGAAC TCGGCGGTAA CTGCTCCTGC ATCTAACTTG GCTGTTGTCC 801 CTCCACTCCT GCCCTTGGGG TGTCTGCAGC AGGCTGCTGC CTAGGCCTGG 851 ACACATTGCA CATCCTAAAG TTTGAAGAGT CTAAATAACG GGGCTTCCCT 901 CAGCATGTTC CCTCTCCTGT TTGCCACGGA TCCAGAGCCA CCTGCCCTGT 951 CTTCTCGTAC CCCTTTCACT CTTGAGGCCT GGGAGGTGAA AAAGGCCAGA 1001 CTGTGCCCAG GATTGATTCA ATTTTGCTTT TACTCCCAGC TTCCCTCTCA 1051 AAAGAGAGTG AAGTCTCATT TGTCATGTGT CTTCAGTTCC CCAACTTGGC 1101 ATGAACATTT GAACCAAACA TAGGAAACTA CCATTAGGTT GAAAGCCTGA 1151 GGCAGCTGGG ATGGTCTTTC TTGTGTCTCT TCTTTGCACC CCAGAGCATG 1201 ATATAAGTGG TCCTAACAGA TTCTGGATAA TGGAGAAGCC CTCTGCTGGT 1251 TTTCCTGGCA TTCCATGTAG AATAGGTAGA GAATATTTAA CCAATGAGCA 1301 AATAAATGTT GGCATGTTTC ATGAAAAAAA AAAAAAAAAA A BLAST Results o BLAST result Medlme entries o Medlme entry Peptide information for frame 2 ORF from 479 bp to 841 bp; peptide length: 121 Category: questionable ORF Classification: no clue 1 MRGTRCLAEY HLGDYGHAWN RCWVLDRVDT WAVVMFIDFG QLATIPVQSL 51 RQLDSDDFWT IPPLTQPFML EKDILSSYEV VHRILKGKIT GALNSAVTAP 101 ASNLAVVPPL LPLGCLQQAA A BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_2m20, frame 2 No Alert BLASTP hits found Peptide information for frame 3 ORF from 87 bp to 635 bp; peptide length: 183 Category: putative protein Classification: no clue 1 MIQQPRAPLV LEKASGEGFG KTAAIIQLAP KAPVDLCETE KLRAAFFAVP 51 LEMRGSFLVL LLRECFRDLS WLALIHSVRG EAGLLVTΞIV PKTPFFWAMH 101 ITEALHQNMQ ALFSTLAQAE EQQPYLEAPP LCAGLAVWQS TTWGIMDTPG 151 TGVGCWTGWT PGLWSCSLIL DSWPPSLCSL CAS BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_2m20, frame 3 No Alert BLASTP hits found Pedant information for DKFZphtes3_2m20, frame 2 Report for DKFZphtes3_2m20.2 [LENGTH] 121 [MW] 13436.69 [pi] 5.81 [KW] Alpha Beta SEQ MRGTRCLAEYHLGDYGHAWNRCWVLDRVDTWAVVMFIDFGQLATIPVQSLRQLDSDDFWT PRD ccchhhhhcccccccccccceeeecccccccceeeeeecccccccccccccccccccccc SEQ IPPLTQPFMLEKDILΞΞYEVVHRILKGKITGALNSAVTAPASNLAVVPPLLPLGCLQQAA PRD cccccchhhhhhhcchhhhhhhhhhcccccchhhhhhcccccceeeeccccccccccccc SEQ A PRD c (No Prosite data available for DKFZphtes3_2m20.2) (No Pfam data available for DKFZphtes3_2m20.2) Pedant information for DKFZphtes3_2m20, frame 3 Report for DKFZphtes3_2m20.3 [LENGTH] 183 [MW] 19971.49 [pi] 5.31 [KW] Alpha Beta SEQ MIQQPRAPLVLEKAΞGEGFGKTAAIIQLAPKAPVDLCETEKLRAAFFAVPLEMRGSFLVL PRD ccccccccceeeecccccccccccccccccccccchhhhhhhhhhhhhhhhhcchhhhhh SEQ LLRECFRDLSWLALIHSVRGEAGLLVTSIVPKTPFFWAMHITEALHQNMQALFSTLAQAE PRD hhhhhhcchhhhhhhhhhcccceeeeeeeccccchhhhhhhhhhhhhhhhhhhhhhhhhh SEQ EQQPYLEAPPLCAGLAVWQSTTWGIMDTPGTGVGCWTGWTPGLWSCSLILDSWPPSLCSL PRD hhhcccccccccccceeeecccceeecccccccccccccccccccceeeeccccccceee SEQ CAS PRD CCC (No Prosite data available for DKFZphtes3_2m20.3) (No Pfam data available for DKFZphtes3_2m20.3) DKFZphtes3_2n9 group: testes derived DKFZphtes3_2n9 encodes a novel 184 ammo acid protein with very weak similarity to Homo sapiens PAC clone DJ0771P04 from 7qll .21-qll .23. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . unknown on genomic level encoded by HS1186N24, no splice pattern but EST matches Sequenced by EMBL Locus : unknown Insert length: 1000 bp Poly A stretch at pos. 988, polyadenylation signal at pos. 970 1 CAACTTTTTA AAGATGTGAA TTGGACAGCC AGACTTGCTT ATTTGTCTGA 51 TATCTTCAGT ATTTTTTAAT GATCTTAATG CTTCTATGCA AGGGAAGAAT 101 GCAACTTATT TTTCAATGGC AGATAAAGTT GAAGGACAAA AACAGAAGTT 151 AGAAGCTTGG AAAAACAGAA TTTCTACAGA TTGTTATGAC ATGTTTCATA 201 ATTTAACAAC AATTATCAAT GAAGTAGGTA ATGATCTTGA TATTGCACAT 251 CTGCGAAAAG TTATCAGTGA ACATCTTACA AATTTGTTAG AATGTTTTGA 301 ATTTTATTTT CCATCAAAAG AAGATCCACG CATAGGAAAT TTGTGGATCC 351 AAAATCCATT TCTTTCATCA AAAGATAACT TAAATTTAAC TGTAACTCTA 401 CAGGATAAGT TGTTGAAGCT GGCTACCGAC GAAGGATTGA AAATCAGTTT 451 TGAAAATACA GCATCACTTC CTTCATTTTG GATAAAAGCT AAAAATGACT 501 ATCCTGAGCT TGCTGAGATT GCTTTAAAAT TGCTGCTTCT TTTCCCCTCA 551 ACATACCTCT GTGAGACCGG ATTCTCTACT TTAAGTGTTA TTAAAACAAA 601 ACATAGAAAC AGTTTAAATA TACATTATCC CCTGAGGTAG CATTGTCATC 651 AATCCAACCT AGATTAGACA AATTAACAAG CAAGAAGCAA GCTCACTTAT 701 CACATTAAAA GCTTTAAATA TTGATATGTA AGGTATTGGT TCAAAGTATG 751 CATATAAGCA TTGAGTGTGA GGAATTTGCT ATTTCACTTT AAACTTTCTG 801 TCTAGTTACA GTTATGGAAG TATGAGAAGT TATGAGTGAA ACAGCAATTT 851 TCTATATAAA TTGCCTATAT GTATATTTTC AATTAAGAAT GTGTACAGTT 901 TTTATAATTC TATTTTTCCT CATATTTGTC GTATTTATTA AAATATAATT 951 TTAAATCTGT TGATTCTAAT ATTAAAACAT TTGATCTTAA AAAAAAAAAA BLAST Results Entry HS1186N24 from database EMBLNEW: Human DNA sequence *** SEQUENCING IN PROGRESS *** from clone 1186N24 Score = 4921, P = 5.8e-215, identities = 989/992 Medline entries No Medlme entry Peptide information for frame 2 ORF from 86 bp to 637 bp; peptide length: 184 Category: similarity to unknown protein Classification: no clue 1 MQGKNATYFS MADKVEGQKQ KLEAWKNRIS TDCYDMFHNL TTIINEVGND 51 LDIAHLRKVI SEHLTNLLEC FEFYFPSKED PRIGNLWIQN PFLSSKDNLN 101 LTVTLQDKLL KLATDEGLKI SFENTASLPS FWIKAKNDYP ELAEIALKLL 151 LLFPSTYLCE TGFSTLSVIK TKHRNSLNIH YPLR BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_2n9, frame 2 TREMBLNEW:AC004883_3 gene: "WUGSC :H_DJ0771P04.2"; Homo sapiens PAC clone DJ0771P04 from 7qll .21-qll .23, complete sequence., N = 1, Score = 94, P = 0.042 >TREMBLNEW:AC004883_3 gene: "WUGSC :H_DJ0771P04.2"; Homo sapiens PAC clone DJ0771P04 from 7qll .21-qll .23, complete sequence. Length = 533 HSPs: Score = 94 (14.1 bits), Expect = 4.3e-02, P = 4.2e-02 Identities = 39/177 (22%), Positives = 75/177 (42%) Query: 1 MQGKNATYFSMADKVEGQKQKLEAWKNRISTDCYDMFHNLTTIINEVGNDLD-IAHLRKV 59 +QG + M D + KL W+ ++ + F L + L+ I + ++ Sbjct: 354 LQGHSQIVTQMYDLIRAFLAKLCLWETHLTRNNLAHFPTLKLASRNESDGLNYIPKIAEL 413 Query: 60 ISEHLTNLLECFEFYFPSKEDPRIGNLWIQNPFLSSKDNLNLTVTLQDKLLKLATDEGLK 119 +E L + F+ Y + + + +PF + D+++ LQ +++ L + LK Sbjct: 414 KTEFQKRLSD-FKLY ESELTL FSSPFSTKIDSVH—EELQMEVIDLQCNTVLK 4_63 Query: 120 ISFENTASLPSFWIKAKNDYPXXXXXXXXXXXXFPSTYLCETGFSTLSVIKTKHRNSL 177 ++ +P F+ YP F STY+CE FS + + KTK+ + L Sbjct: 464 TKYDKVG-IPEFYKYLWGSYPKYKHHCAKILSMFGSTYICEQLFSIMKLSKTKYCSQL 520 Pedant information for DKFZphtes3_2n9, frame 2 Report for DKFZphtes3_2n9.2 [LENGTH] 184 [MW] 21203.53 [pi] 6.52 [KW] Alpha_Beta [KW] LOW_COMPLEXITY 6.52 % SEQ MQGKNATYFSMADKVEGQKQKLEAWKNRISTDCYDMFHNLTTIINEVGNDLDIAHLRKVI SEG PRD ccccccchhhhhhhhhhhhhhhhhhhhhhcchhhhhcccceeecccccccchhhhhhhhh SEQ SEHLTNLLECFEFYFPSKEDPRIGNLWIQNPFLSSKDNLNLTVTLQDKLLKLATDEGLKI SEG PRD hhhhhhhhhhhhcccccccccccceeeeccccccccccceeeeehhhhhhhhhhhcccee SEQ SFENTASLPSFWIKAKNDYPELAEIALKLLLLFPSTYLCETGFSTLSVIKTKHRNSLNIH SEG xxxxxxxxxxxx PRD eecccccccceeeeecccchhhhhhhhhhhhhcccccccccccceeeeeecccccceeec SEQ YPLR SEG .... PRD cccc (No Prosite data available for DKFZphtes3_2n9.2) (No Pfam data available for DKFZphtes3_2n9.2) DKFZphtes3_30f4 group: testes derived DKFZphtes3_30f4 encodes a novel 192 amino acid protein without similarity to known proteins. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . unknown Sequenced by LMU Locus: /map="717.2-8 cR from top of Chr8 linkage group" Insert length: 1388 bp Poly A stretch at pos. 1330, polyadenylation signal at pos. 1310 1 CACTGAGCCC TCCTCAGATG GTTAGTGGCT TCCAACAGCC ATCAGGAGTG 51 TTTCTTGAAT GCCCCAGGTG TGGAGGACTT GGTCTGTGAC CACCTAGAAC 101 CCCAGAGCTG AACAGGAAGC CGTCCCTGCA GCAACAAGAG GGCTGGAAGG 151 GGGAGCTGCA GGCCACCCTC GGCTCTCCCA CTGCTGGGGC GGTGATGTTC 201 GGGTGACATG TTTGAAAAAT ACTCTTAAAG ATACCAACTG TTCCCTTATA 251 TGGCTAATGG TTTGTGCAGC CACCAGCGAT GGCGGCCCCT ATTAGAGACC 301 AGGTTTGTTA AAACACCAAA TATTGCTGTC CACACTAGAC ATTAACCGGC 351 TTCAGAAAAG ATGGACACCT TTTCCCACGC TGTTTCGCTT CTTAACTTTG 401 GTCCAGCTTT AGCCACCACA CAGCGTGTGA GGGACTGCTG CTGCGGAGTC 451 AGCCTCGTTT GTCCCTCCGC CTCCCACCAG CATGCGCCGC TTCTGAGAGA 501 CACCAGCTCC CTGCCTCCAA GCCTGGTGCC ACAGGCCTGT CGTGAGGGAC 551 CCCTGCTTCC GAGAGCTCCT GGGGGGGTTC TGCCCTTCAC CACCTGGGAG 601 AGGTGTCAGT TCAGTTCCGA GTTGAACAAG GCCCGTGCAC ACAGCATGTT 651 GGGGGCCCAG CCCAAAGTTC TTGTCACCTC CTCATGCAAA GCCAGCCATC 701 ACCCTCCGGC CAGAGCTCAA GGTGGCCCCT TGGCCAGCCC CTCCTTGGGT 751 CCTCCAGGAG GACTGAGCAC CCCTCCTAGC GGCATCCCTT GCCCTCCACA 801 GTGCTGCCAG GGGCACGTCG CTCTGTGCCG TGGACTGAGA CCATCCCCTG 851 GTGACAGAAT GACCCGTTTG TTGGAAATGC CTCGTTGCCA GAGAAACTCC 901 CCAGGCATCT CGGAACGAAA CTATTTAGTT CCATTGTGAA CTGGCCACGG 951 GACAGCTTTT TATCAACTTA TTAAGTTGGA GCACTGTAAT CGCGCTTGCT 1001 GAGTTAGCAG TGGTGGTAAG CGTGTGTTAA ACACATAATG TTACGTTTTA 1051 GGAGAGAGAG GTCGTAAGGA AGTGTCGTGT CGCTCATGAC TCTCTTCTAT 1101 TAGTTGGGTA ACAGTGGCCT CATGTTTGTG TCTGTGTGTA CACAGAGCCC 1151- TTAGGTTCTG CTCTGTTTCT TTGCCAGGTG AATGTTTGTG GCATGCGCTG 1201 CTGTCCGCGC CCCTCTGTCC TGCGCAGGGT TCAGCTGTGC GGCGCCCTGA 1251 TTTCCTCCAT GCACACAGAA CCTCCTTGTG TCTGTTTCTC TGTTCCTCTG 1301 TGGCTGACTC AATAAACTTT TCCCTCTGAC ATGAAAAAAA AAAAAAAAAA 1351 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAG BLAST Results Entry HS548358 from database EMBL: human STS EST67250. Score = 2126, P = 1.5e-89, identities = 444/472 Entry HS670351 from database EMBL: human STS WI-18501. Score = 2089, P = 7.1e-88, identities = 445/476 Medlme entries No Medline entry Peptide information for frame 1 ORF from 361 bp to 936 bp; peptide length: 192 Category: putative protein Classification: no clue 1 MDTFSHAVSL LNFGPALATT QRVRDCCCGV SLVCPSASHQ HAPLLRDTSS 51 LPPSLVPQAC REGPLLPRAP GGVLPFTTWE RCQFΞSELNK ARAHSMLGAQ 101 PKVLVTSSCK ASHHPPARAQ GGPLASPSLG PPGGLSTPPS GIPCPPQCCQ 151 GHVALCRGLR PSPGDRMTRL LEMPRCQRNS PGISERNYLV PL BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_30f4, frame 1 No Alert BLASTP hits found Pedant information for DKFZphtes3_30f4, frame 1 Report for DKFZphtes3_30f4.1 [LENGTH] 192 [MW] 20281.56 [pi] 9.21 [BLOCKS] BL01013C Oxysterol-binding protein family proteins [KW] All_Alpha [KW] LOW COMPLEXITY 10.94 % SEQ MDTFSHAVSLLNFGPALATTQRVRDCCCGVSLVCPSASHQHAPLLRDTSSLPPSLVPQAC SEG PRD ccchhhhheeecccccchhhhhhhhcccceeeeccccccccccccccccccccccccccc SEQ REGPLLPRAPGGVLPFTTWERCQFSSELNKARAHSMLGAQPKVLVTSSCKASHHPPARAQ SEG PRD cccccccccccccccccccccchhhhhhhhhhhhhhccccceeeeecccccccccccccc SEQ GGPLASPSLGPPGGLSTPPSGIPCPPQCCQGHVALCRGLRPSPGDRMTRLLEMPRCQRNS SEG xxxxxxxxxxxxxxxxxxxxx PRD cccccccccccccccccccccccccccccchhhhhhhhcccccccchhhhhccccccccc SEQ PGISERNYLVPL SEG PRD cccccccccccc (No Prosite data available for DKFZphtes3_30f4.1) (No Pfam data available for DKFZphtes3_30f4.1) DKFZphtes3_35b4 group: cell cycle DKFZphtes3_35b4 encodes a novel 1780 amino acid protein which is C-terminal identical to human M-phase phosphoprotem-1 (MPPl). The novel protein contains a N-termmal Pfam kinesm motor domain and a ATP/GTP-bindmg site motif A (P-loop). MPPl is expressed and phosphorylated in the metaphase. Therefore the novel protein seems to be involved in the mitotic spindle during cell division. The new protein can find application in modulation of the mitotic spindle. "M-phase phosphoproteιn-1" extension motor protein Sequenced by DKFZ Locus: /map="750_H_l; 758_H_7; 759_C_9; 8 7_D_4; 906_D_1; 931_D_3; 944_C_1; 750_G_12; 800_A_11; 512.1 cR from top of ChrlO linkage group" Insert length: 6284 bp No poly A stretch found, no polyadenylation signal found 1 ATCGCAGTGC TGCTCGCGGG TCTGGCTAGT CAGGCGAAGT TTGCAGAATG 51 GAATCTAATT TTAATCAAGA GGGAGTACCT CGACCATCTT ATGTTTTTAG 101 TGCTGACCCA ATTGCAAGGC CTTCAGAAAT AAATTTCGAT GGCATTAAGC 151 TTGATCTGTC TCATGAATTT TCCTTAGTTG CTCCAAATAC TGAGGCAAAC 201 AGTTTCGAAT CTAAAGATTA TCTCCAGGTT TGTCTTCGAA TAAGACCATT 251 TACACAGTCA GAAAAAGAAC TTGAGTCTGA GGGCTGTGTG CATATTCTGG 301 ATTCACAGAC TGTTGTGCTG AAAGAGCCTC AATGCATCCT TGGTCGGTTA 351 AGTGAAAAAA GCTCAGGGCA GATGGCACAG AAATTCAGTT TTTCCAAGGT 401 TTTTGGCCCA GCAACTACAC AGAAGGAATT CTTTCAGGGT TGCATTATGC 451 AACCAGTAAA AGACCTCTTG AAAGGACAGA GTCGTCTGAT TTTTACTTAC 501 GGGCTAACCA ATTCAGGAAA AACATATACA TTTCAAGGGA CAGAAGAAAA 551 TATTGGCATT CTGCCTCGAA CTTTGAATGT ATTATTTGAT AGTCTTCAAG 601 AAAGACTGTA TACAAAGATG AACCTTAAAC CACATAGATC CAGAGAATAC 651 TTAAGGTTAT CATCAGAACA AGAGAAAGAA GAAATTGCTA GCAAAAGTGC 701 ATTGCTTCGG CAAATTAAAG AGGTTACTGT GCATAATGAT AGTGATGATA 751 CTCTTTATGG AAGTTTAACT AACTCTTTGA ATATCTCAGA GTTTGAAGAA 801 TCCATAAAAG ATTATGAACA AGCCAACTTG AATATGGCTA ATAGTATAAA 851 ATTTTCTGTG TGGGTTTCTT TCTTTGAAAT TTACAATGAA TATATTTATG 901 ACTTATTTGT TCCTGTATCA TCTAAATTCC AAAAGAGAAA GATGCTGCGC 951 CTTTCCCAAG ACGTAAAGGG CTATTCTTTT ATAAAAGATC TACAATGGAT 1001 TCAAGTATCT GATTCCAAAG AAGCCTATAG ACTTTTAAAA CTAGGAATAA 1051 AGCACCAGAG TGTTGCCTTC ACAAAATTGA ATAATGCTTC CAGTAGAAGT 1101 CACAGCATAT TCACTGTTAA AATATTACAG ATTGAAGATT CTGAAATGTC 1151 TCGTGTAATT CGAGTCAGTG AATTATCTTT ATGTGATCTT GCTGGTTCAG 1201 AACGAACTAT GAAGACACAG AATGAAGGTG AAAGGTTAAG AGAGACTGGG 1251 AATATCAACA CTTCTTTATT GACTCTGGGA AAGTGTATTA ACGTCTTGAA 1301 GAATAGTGAA AAGTCAAAGT TTCAACAGCA TGTGCCTTTC CGGGAAAGTA 1351 AACTGACTCA CTATTTTCAA AGTTTTTTTA ATGGTAAAGG GAAAATTTGT 1401 ATGATTGTCA ATATCAGCCA ATGTTATTTA GCCTATGATG AAACACTCAA 1451 TGTATTGAAG TTCTCCGCCA TTGCACAAAA AGTTTGTGTC CCAGACACTT 1501 TAAATTCCTC TCAAGATAAA TTATTTGGAC CTGTCAAATC TTCTCAAGAT 1551 GTATCACTAG ACAGTAATTC AAACAGTAAA ATATTAAATG TAAAAAGAGC 1601 CACCATTTCA TGGGAAAATA GTCTAGAAGA TTTGATGGAA GACGAGGATT 1651 TGGTTGAGGA GCTAGAAAAC GCTGAAGAAA CTCAAAATGT GGAAACTAAA 1701 CTTCTTGATG AAGATCTAGA TAAAACATTA GAGGAAAATA AGGCTTTCAT 1751 TAGCCACGAG GAGAAAAGAA AACTGTTGGA CTTAATAGAA GACTTGAAAA 1801 AAAAACTGAT AAATGAAAAA AAGGAAAAAT TAACCTTGGA ATTTAAAATT 1851 CGAGAAGAAG TTACACAGGA GTTTACTCAG TATTGGGCTC AACGGGAAGC 1901 TGACTTTAAG GAGACTCTGC TTCAAGAACG AGAGATATTA GAAGAAAATG 1951 CTGAACGTCG TTTGGCTATC TTCAAGGATT TGGTTGGTAA ATGTGACACT 2001 CGAGAAGAAG CAGCGAAAGA CATTTGTGCC ACAAAAGTTG AAACTGAAGA 2051 AGCTACTGCT TGTTTAGAAC TAAAGTTTAA TCAAATTAAA GCTGAATTAG 2101 CTAAAACCAA AGGAGAATTA ATCAAAACCA AAGAAGAGTT AAAAAAGAGA 2151 GAAAATGAAT CAGATTCATT GATTCAAGAG CTTGAGACAT CTAATAAGAA 2201 AATAATTACA CAGAATCAAA GAATTAAAGA ATTGATAAAT ATAATTGATC 2251 AAAAAGAAGA TACTATCAAC GAATTTCAGA ACCTAAAGTC TCATATGGAA 2301 AACACATTTA AATGCAATGA CAAGGCTGAT ACATCTTCTT TAATAATAAA 2351 CAATAAATTG ATTTGTAATG AAACAGTTGA AGTACCTAAG GACAGCAAAT 2401 CTAAAATCTG TTCAGAAAGA AAAAGAGTAA ATGAAAATGA ACTTCAGCAA 2451 GATGAACCAC CAGCAAAGAA AGGGTCTATC CATGTTAGTT CAGCTATCAC 2501 TGAAGACCAA AAGAAAAGTG AAGAAGTGCG ACCGAACATT GCAGAAATTG 2551 AAGACATCAG AGTTTTACAA GAAAATAATG AAGGACTGAG AGCATTTTTA 2601 CTCACTATTG AGAATGAACT TAAAAATGAA AAGGAAGAAA AAGCAGAATT 2651 AAATAAACAG ATTGTTCATT TTCAGCAGGA ACTTTCTCTT TCTGAAAAAA 2701 AGAATTTAAC TTTAAGTAAA GAGGTCCAAC AAATTCAGTC AAATTATGAT 2751 ATTGCAATTG CTGAATTACA TGTGCAGAAA AGTAAAAATC AAGAACAGGA 2801 GGAAAAGATC ATGAAATTGT CAAATGAGAT AGAAACTGCT ACAAGAAGCA 2851 TTACAAATAA TGTTTCACAA ATAAAATTAA TGCACACGAA AATAGACGAA 2901 CTACGTACTC TTGATTCAGT TTCTCAGATT TCAAACATAG ATTTGCTCAA 2951 TCTCAGGGAT CTGTCAAATG GTTCTGAGGA GGATAATTTG CCAAATACAC 3001 AGTTAGACCT TTTAGGTAAT GATTATTTGG TAAGTAAGCA AGTTAAAGAA 3051 TATCGAATTC AAGAACCCAA TAGGGAAAAT TCTTTCCACT CTAGTATTGA 3101 AGCTATTTGG GAAGAATGTA AAGAGATTGT GAAGGCCTCT TCCAAAAAAA 3151 GTCATCAGAT TGAGGAACTG GAACAACAAA TTGAAAAATT GCAGGCAGAA 3201 GTAAAAGGCT ATAAGGATGA AAACAATAGA CTAAAGGAGA AGGAGCATAA 3251 AAACCAAGAT GACCTACTAA AAGAAAAAGA AACTCTTATA CAGCAGCTGA 3301 AAGAAGAATT GCAAGAAAAA AATGTTACTC TTGATGTTCA AATACAGCAT 3351 GTAGTTGAAG GAAAGAGAGC GCTTTCAGAA CTTACACAAG GTGTTACTTG 3401 CTATAAGGCA AAAATAAAGG AACTTGAAAC AATTTTAGAG ACTCAGAAAG 3451 TTGAACGTAG TCATTCAGCC AAGTTAGAAC AAGACATTTT GGAAAAGGAA 3501 TCTATCATCT TAAAGCTAGA AAGAAATTTG AAGGAATTTC AAGAACATCT 3551 TCAGGATTCT GTCAAAAACA CCAAAGATTT AAATGTAAAG GAACTCAAGC 3601 TGAAAGAAGA AATCACACAG TTAACAAATA ATTTGCAAGA TATGAAACAT 3651 TTACTTCAAT TAAAAGAAGA AGAAGAAGAA ACCAACAGGC AAGAAACAGA 3701 AAAATTGAAA GAGGAACTCT CTGCAAGCTC TGCTCGTACC CAGAATCTGA 3751 AAGCAGATCT TCAGAGGAAG GAAGAAGATT ATGCTGACCT GAAAGAGAAA 3801 CTGACTGATG CCAAAAAGCA GATTAAGCAA GTACAGAAAG AGGTATCTGT 3851 AATGCGTGAT GAGGATAAAT TACTGAGGAT TAAAATTAAT GAACTGGAGA 3901 AAAAGAAAAA CCAGTGTTCT CAGGAATTAG ATATGAAGCA GCGAACCATT 3951 CAGCAACTCA AGGAGCAGTT AAATAATCAG AAAGTGGAAG AAGCTATACA 4001 ACAGTATGAG AGAGCATGCA AAGATCTAAA TGTTAAAGAG AAAATAATTG 4051 AAGACATGCG AATGACACTA GAAGAACAGG AACAAACTCA GGTAGAACAG 4101 GATCAAGTGC TTGAGGCTAA ATTAGAGGAA GTTGAAAGGC TGGCCACAGA 4151 ATTGGAAAAA TGGAAGGAAA AATGCAATGA TTTGGAAACC AAAAACAATC 4201 AAAGGTCAAA TAAAGAACAT GAGAACAACA CAGATGTGCT TGGAAAGCTC 4251 ACTAATCTTC AAGATGAGTT ACAGGAGTCT GAACAGAAAT ATAATGCTGA 4301 TAGAAAGAAA TGGTTAGAAG AAAAAATGAT GCTTATCACT CAAGCGAAAG 4351 AAGCAGAGAA TATACGAAAT AAAGAGATGA AAAAATATGC TGAGGACAGG 4401 GAGCGTTTTT TTAAGCAACA GAATGAAATG GAAATACTGA CAGCCCAGCT 4451 GACAGAGAAA GATAGTGACC TTCAAAAGTG GCGAGAAGAA CGAGATCAAC 4501 TGGTTGCAGC TTTAGAAATA CAGCTAAAAG CACTGATATC CAGTAATGTA 4551 CAGAAAGATA ATGAAATTGA ACAACTAAAA AGGATCATAT CAGAGACTTC 4601 TAAAATAGAA ACACAAATCA TGGATATCAA GCCCAAACGT ATTAGTTCAG 4651 CAGATCCTGA CAAACTTCAA ACTGAACCTC TATCGACAAG TTTTGAAATT 4701 TCCAGAAATA AAATAGAGGA TGGATCTGTA GTCCTTGACT CTTGTGAAGT 4751 GTCAACAGAA AATGATCAAA GCACTCGATT TCCAAAACCT GAGTTAGAGA 4801 TTCAATTTAC ACCTTTACAG CCAAACAAAA TGGCAGTGAA ACACCCTGGT 4851 TGTACCACAC CAGTGACAGT TGAGATTCCC AAGGCTCGGA AGAGGAAGAG 4901 TAATGAAATG GAGGAGGACT TGGTGAAATG TGAAAATAAG AAGAATGCTA 4951 CACCCAGAAC TAATTTGAAA TTTCCTATTT CAGATGATAG AAATTCTTCT 5001 GTCAAAAAGG AACAAAAGGT TGCCATACGT CCATCATCTA AGAAAACATA 5051 TTCTTTACGG AGTCAGGCAT CCATAATTGG TGTAAACCTG GCCACTAAGA 5101 AAAAAGAAGG AACACTACAG AAATTTGGAG ACTTCTTACA ACATTCTCCC 5151 TCAATTCTTC AATCAAAAGC AAAGAAGATA ATTGAAACAA TGAGCTCTTC 5201 AAAGCTCTCA AATGTAGAAG CAAGTAAAGA AAATGTGTCT CAACCAAAAC 5251 GAGCCAAACG GAAATTATAC ACAAGTGAAA TTTCATCTCC TATTGATATA 5301 TCAGGCCAAG TGATTTTAAT GGACCAGAAA ATGAAGGAGA GTGATCACCA 5351 GATTATCAAA CGACGACTTC GAACAAAAAC AGCCAAATAA ATCACTTATG 5401 GAAATGTTTA ATATAAATTT TATAGTCATA GTCATTGGAA CTTGCATCCT 5451 GTATTGTAAA TATAAATGTA TATATTATGC ATTAAATCAC TCTGCATATA 5501 GATTGCTGTT TTATACATAG TATAATTTTA ATTCAATAAA TGAGTCAAAA 5551 TTTGTATATT TTTATAAGGC TTTTTTATAA TAGCTTCTTT CAAACTGTAT 5601 TTCCCTATTA TCTCAGACAT TGGATCAGTG AAGATCCTAG GAAAGAGGCT 5651 GTTATTCTCA TTTATTTTGC TATACAGGAT GTAATAGGTC AGGTATTTGG 5701 TTTACTTATA TTTAACAATG TCTTATGAAT TTTTTTTACT TTATCTGTTA 5751 TACAACTGAT TTTACATATC TGTTTGGATT ATAGCTAGGA TTTGGAGAAT 5801 AAGTGTGTAC AGATCACAAA ACATGTATAT ACATTATTTA GAAAAGATCT 5851 CAAGTCTTTA ATTAGAATGT CTCACTTATT TTGTAAACAT TTTGTGGGTA 5901 CATAGTACAT GTATATATTT ACGGGGTATG TGAGATGTTT TGACACAGGC 5951 ATGCAATGTG AAATACGTGT ATCATGGAGA ATGAGGTATC CATCCCCTCA 6001 AGCATTTTTC CTTTGAATTA CAGATAATCC AATTACATTC TTTAGATCAT 6051 TTAAAAATAT ACAAGTAAGT TATTATTGAT TATAGTCACT CTATTGTGCT 6101 ATCAGATAGT AGATCATTCT TTTTATCTTA TTTGTTTTTG TACCCATTAA 6151 CCATCCCCAC CTCCCCCTGC AACCGTCAGT ACCCTTACCA GCCACTGGTA 6201 ACCATTCTTC TACTCTGTAT GCCCATGAGG TCAATTGATT TTATTTTTAG 6251 ATCCCATAAA TAAATGAGAA CATGCAAAAA AAAA BLAST Results Entry HS898149 from database EMBL: human STS WI-9217. Score = 4247, P = 1.5e-187, identities = 855/862 Medline entries 94119956: Cloning of cDNAs for M-phase phosphoproteins recognized by the MPM2 monoclonal antibody and determination of the phosphorylated epitope. 98101856: Interaction of a Golgi-associated kmesin-like protein with Rab6. 95122643: Identification and partial characterization of mitotic centromere-associated kmesin, a kinesin-related protein that associates with centromeres during mitosis . Peptide information for frame 3 ORF from 48 bp to 5387 bp; peptide length: 1780 Category: known protein Classification: Cell structure/motility Prosite motifs: ATP GTP A (152-160) 1 MESNFNQEGV PRPSYVFSAD PIARPSEINF DGIKLDLSHE FSLVAPNTEA 51 NSFESKDYLQ VCLRIRPFTQ SEKELESEGC VHILDSQTVV LKEPQCILGR 101 LSEKSSGQMA QKFSFSKVFG PATTQKEFFQ GCIMQPVKDL LKGQSRLIFT 151 YGLTNSGKTY TFQGTEENIG ILPRTLNVLF DΞLQERLYTK MNLKPHRSRE 201 YLRLSSEQEK EEIASKSALL RQIKEVTVHN DSDDTLYGSL TNSLNISEFE 251 ESIKDYEQAN LNMANSIKFS VWVSFFEIYN EYIYDLFVPV SSKFQKRKML 301 RLSQDVKGYS FIKDLQWIQV SDSKEAYRLL KLGIKHQΞVA FTKLNNASSR 351 SHSIFTVKIL QIEDSEMSRV IRVSELSLCD LAGSERTMKT QNEGERLRET 401 GNINTΞLLTL GKCINVLKNS EKSKFQQHVP FRESKLTHYF QSFFNGKGKI 451 CMIVNISQCY LAYDETLNVL KFSAIAQKVC VPDTLNSSQD KLFGPVKSSQ 501 DVSLDSNSNS KILNVKRATI SWENSLEDLM EDEDLVEELE NAEETQNVET 551 KLLDEDLDKT LEENKAFISH EEKRKLLDLI EDLKKKLINE KKEKLTLEFK 601 IREEVTQEFT QYWAQREADF KETLLQEREI LEENAERRLA IFKDLVGKCD 651 TREEAAKDIC ATKVETEEAT ACLELKFNQI KAELAKTKGE LIKTKEELKK 701 RENESDSLIQ ELETSNKKII TQNQRIKELI NIIDQKEDTI NEFQNLKSHM 751 ENTFKCNDKA DTSSLIINNK LICNETVEVP KDSKSKICSE RKRVNENELQ 801 QDEPPAKKGS IHVSSAITED QKKSEEVRPN IAEIEDIRVL QENNEGLRAF 851 LLTIENELKN EKEEKAELNK QIVHFQQELS LSEKKNLTLS KEVQQIQSNY 901 DIAIAELHVQ KΞKNQEQEEK IMKLSNEIET ATRSITNNVS QIKLMHTKID 951 ELRTLDSVSQ ISNIDLLNLR DLSNGSEEDN LPNTQLDLLG NDYLVSKQVK 1001 EYRIQEPNRE NSFHSSIEAI WEECKEIVKA SΞKKSHQIEE LEQQIEKLQA 1051 EVKGYKDENN RLKEKEHKNQ DDLLKEKETL IQQLKEELQE KNVTLDVQIQ 1101 HVVEGKRALS ELTQGVTCYK AKIKELETIL ETQKVERSHS AKLEQDILEK 1151 ESIILKLERN LKEFQEHLQD SVKNTKDLNV KELKLKEEIT QLTNNLQDMK 1201 HLLQLKEEEE ETNRQETEKL KEELSAΞSAR TQNLKADLQR KEEDYADLKE 1251 KLTDAKKQIK QVQKEVSVMR DEDKLLRIKI NELEKKKNQC SQELDMKQRT 1301 IQQLKEQLNN QKVEEAIQQY ERACKDLNVK EKIIEDMRMT LEEQEQTQVE 1351 QDQVLEAKLE EVERLATELE KWKEKCNDLE TKNNQRSNKE HENNTDVLGK 1401 LTNLQDELQE SEQKYNADRK KWLEEKMMLI TQAKEAENIR NKEMKKYAED 1451 RERFFKQQNE MEILTAQLTE KDSDLQKWRE ERDQLVAALE IQLKALISSN 1501 VQKDNEIEQL KRIIΞETSKI ETQIMDIKPK RISSADPDKL QTEPLSTSFE 1551 ISRNKIEDGS VVLDSCEVST ENDQSTRFPK PELEIQFTPL QPNKMAVKHP 1601 GCTTPVTVEI PKARKRKSNE MEEDLVKCEN KKNATPRTNL KFPISDDRNS 1651 ΞVKKEQKVAI RPSSKKTYSL RSQASIIGVN LATKKKEGTL QKFGDFLQHS 1701 PSILQSKAKK IIETMSSSKL SNVEASKENV SQPKRAKRKL YTSEISSPID 1751 IΞGQVILMDQ KMKESDHQII KRRLRTKTAK BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_35b4, frame 3 TREMBL :U93121_1 product: "M-phase phosphoproteιn-1"; Human M-phase phosphoproteιn-1 mRNA, partial eds., N = 1, Score = 3743, P = 0 PIR:A36881 MPM2-reactιve phosphoprotein 1 - human (fragment), N = 2, Score = 2808, P = 2.5e-294 TREMBL:AF070672_1 product: "rabkinesinδ" ; Homo sapiens rabkmesinδ mRNA, complete eds., N = 2, Score = 680, P = 2.6e-99 >TREMBL:U93121_1 product: "M-phase phosphoproteιn-1"; Human M-phase phosphoproteιn-1 mRNA, partial eds. Length = 753 HSPs: Score = 3743 (561.6 bits), Expect = O.Oe+00, P = O.Oe+00 Identities = 752/753 (99%), Positives = 753/753 (100%) Query: 1028 VKASSKKSHQIEELEQQIEKLQAEVKGYKDENNRLKEKEHKNQDDLLKEKETLIQQLKEE 1087 VKASΞKKΞHQIEELEQQIEKLQAEVKGYKDENNRLKEKEHKNQDDLLKEKETLIQQLKEE Sbjct: 1 VKASSKKSHQIEELEQQIEKLQAEVKGYKDENNRLKEKEHKNQDDLLKEKETLIQQLKEE 60 Query: 1088 LQEKNVTLDVQIQHVVEGKRALSELTQGVTCYKAKIKELETILETQKVERSHSAKLEQDI 1147 LQEKNVTLDVQIQHVVEGKRALSELTQGVTCYKAKIKELETILETQKVERSHSAKLEQDI Sbjct: 61 LQEKNVTLDVQIQHVVEGKRALSELTQGVTCYKAKIKELETILETQKVERΞHSAKLEQDI 120 Query: 1148 LEKESIILKLERNLKEFQEHLQDSVKNTKDLNVKELKLKEEITQLTNNLQDMKHLLQLKE 1207 LEKESIILKLERNLKEFQEHLQDSVKNTKDLNVKELKLKEEITQLTNNLQDMKHLLQLKE Sbjct: 121 LEKESIILKLERNLKEFQEHLQDSVKNTKDLNVKELKLKEEITQLTNNLQDMKHLLQLKE 180 Query: 1208 EEEETNRQETEKLKEELSASSARTQNLKADLQRKEEDYADLKEKLTDAKKQIKQVQKEVS 1267 EEEETNRQETEKLKEELSASSARTQNLKADLQRKEEDYADLKEKLTDAKKQIKQVQKEVS Sbjct: 181 EEEETNRQETEKLKEELSASSARTQNLKADLQRKEEDYADLKEKLTDAKKQIKQVQKEVS 240 Query: 1268 VMRDEDKLLRIKINELEKKKNQCSQELDMKQRTIQQLKEQLNNQKVEEAIQQYERACKDL 1327 VMRDEDKLLRIKINELEKKKNQCSQELDMKQRTIQQLKEQLNNQKVEEAIQQYERACKDL Sbjct: 241 VMRDEDKLLRIKINELEKKKNQCSQELDMKQRTIQQLKEQLNNQKVEEAIQQYERACKDL 300 Query: 1328 NVKEKIIEDMRMTLEEQEQTQVEQDQVLEAKLEEVERLATELEKWKEKCNDLETKNNQRS 1387 NVKEKIIEDMRMTLEEQEQTQVEQDQVLEAKLEEVERLATELEKWKEKCNDLETKNNQRS Sbjct: 301 NVKEKIIEDMRMTLEEQEQTQVEQDQVLEAKLEEVERLATELEKWKEKCNDLETKNNQRS 360 Query: 1388 NKEHENNTDVLGKLTNLQDELQESEQKYNADRKKWLEEKMMLITQAKEAENIRNKEMKKY 1447 NKEHENNTDVLGKLTNLQDELQESEQKYNADRKKWLEEKMMLITQAKEAENIRNKEMKKY Sbjct: 361 NKEHENNTDVLGKLTNLQDELQESEQKYNADRKKWLEEKMMLITQAKEAENIRNKEMKKY 420 Query: 1448 AEDRERFFKQQNEMEILTAQLTEKDSDLQKWREERDQLVAALEIQLKALISSNVQKDNEI 1507 AEDRERFFKQQNEMEILTAQLTEKDSDLQKWREERDQLVAALEIQLKALISSNVQKDNEI Sbjct: 421 AEDRERFFKQQNEMEILTAQLTEKDSDLQKWREERDQLVAALEIQLKALISSNVQKDNEI 480 Query: 1508 EQLKRIISETΞKIETQIMDIKPKRISSADPDKLQTEPLSTSFEISRNKIEDGSVVLDSCE 1567 EQLKRIISETSKIETQIMDIKPKRISSADPDKLQTEPLSTSFEISRNKIEDGSVVLDSCE Sbjct: 481 EQLKRIISETSKIETQIMDIKPKRISSADPDKLQTEPLSTSFEISRNKIEDGSVVLDSCE 540 Query: 1568 VSTENDQSTRFPKPELEIQFTPLQPNKMAVKHPGCTTPVTVEIPKARKRKSNEMEEDLVK 1627 VSTENDQSTRFPKPELEIQFTPLQPNKMAVKHPGCTTPVTV+IPKARKRKSNEMEEDLVK Sbjct: 541 VSTENDQSTRFPKPELEIQFTPLQPNKMAVKHPGCTTPVTVKIPKARKRKSNEMEEDLVK 600 Query: 1628 CENKKNATPRTNLKFPISDDRNSΞVKKEQKVAIRPSSKKTYSLRSQASIIGVNLATKKKE 1687 CENKKNATPRTNLKFPISDDRNSSVKKEQKVAIRPSSKKTYSLRΞQASIIGVNLATKKKE Sbjct: 601 CENKKNATPRTNLKFPISDDRNSSVKKEQKVAIRPSSKKTYSLRSQASIIGVNLATKKKE 660 Query: 1688 GTLQKFGDFLQHSPSILQSKAKKIIETMSSSKLSNVEAΞKENVSQPKRAKRKLYTSEISS 1747 GTLQKFGDFLQHSPSILQSKAKKIIETMSSSKLSNVEASKENVSQPKRAKRKLYTSEISS Sbjct: 661 GTLQKFGDFLQHSPSILQSKAKKIIETMSSSKLSNVEASKENVSQPKRAKRKLYTΞEISS 720 Query: 1748 PIDISGQVILMDQKMKESDHQIIKRRLRTKTAK 1780 PIDIΞGQVILMDQKMKESDHQIIKRRLRTKTAK Sbjct: 721 PIDISGQVILMDQKMKESDHQIIKRRLRTKTAK 753 Score = 197 (29.6 bits), Expect = 2. le-11, P = 2. le-11 Identities = 114/542 (21%), Positives = 253/542 (46%) Query: 692 IKTKEELKKRENESDSLIQELETSNKKIITQNQRIKELINIIDQKEDTINEFQNLKSHM- 750 +K + + E + I++L+ K +N R+KE + ++D + E + L + Sbjct: 1 VKASSKKSHQIEELEQQIEKLQAEVKGYKDENNRLKEKEH—KNQDDLLKEKETLIQQLK 58 Query: 751 ENTFKCNDKADTS-SLIINNKLICNETVEVPKDSKSKICSERKRVNENELQQDEPPAK— 807 E + N D ++ K +E + K+KI E + + E + + AK Sb j ct: 59 EELQEKNVTLDVQIQHVVEGKRALSELTQGVTCYKAKI-KELETILETQKVERSHSAKLE 117 Query: 808 KGSIHVSSAITEDQKKSEEVRPNIAE-IEDIRVLQENNEGLRAFLLTIENELKNEK 862 + + S I + ++ +E + ++ + +++ + L L+ + + N L++ K Sbjct: 118 QDILEKESIILKLERNLKEFQEHLQDSVKNTKDLNVKELKLKEEITQLTNNLQDMKHLLQ 177 Query: 863 —EEKAELNKQIVH-FQQELSLSEKKNLTLSKEVQQIQSNYDIAIAELHVQKSKNQEQEE 919 EE+ E N+Q ++ELΞ S + L ++Q+ + +Y A+L K K + ++ Sb j ct: 178 LKEEEEETNRQETEKLKEELSASSARTQNLKADLQRKEEDY ADL KEKLTDAKK 230 Query: 920 KIMKLSNEIETATRSITNNVSQIKLMHTKIDEL-RTLDSVSQISNIDLLNLRDLSNGSEE 978 +1 ++ E+ S+ + + KL+ KI+EL + + SQ +D+ R + E+ Sbjct: 231 QIKQVQKEV ΞVMRD--EDKLLRIKINELEKKKNQCSQ—ELDMKQ-RTIQQLKEQ 280 Query: 979 DNLPNTQLDLLGNDYLVSKQVKEYRIQEPNRENSFHSSIEAIWEECKEIVKASSKKSHQI 1038 N N +++ Y + K+ ++E E+ ++E + E + K ++ Sbjct: 281 LN—NQKVEEAIQQY—ERACKDLNVKEKIIED-MRMTLEEQEQTQVEQDQVLEAKLEEV 335 Query: 1039 EELEQQIEKLQAEVKGYKDENNRLKEKEHKNQDDLLKEKETLIQQLKEELQEKNVT 1094 E L ++EK + + + +NN+ KEH+N D+L + L +L+E Q+ N Sbjct: 336 ERLATELEKWKEKCNDLETKNNQRΞNKEHENNTDVLGKLTNLQDELQESEQKYNADRKKW 395 Query: 1095 LDVQIQHVVEGKRA LSELTQGVTCYKAKIKELETILETQKVERSHSAKLEQDI 1147 L+ ++ + + K A + + + + + E+E IL Q E+ + ++ Sbjct: 396 LEEKMMLITQAKEAENIRNKEMKKYAEDRERFFKQQNEME-ILTAQLTEKDSDLQKWRE- 453 Query: 1148 LEKESIILKLERNLKEFQEHLQDSVKNTKDLNVKELK-LKEEITQLTNNLQDMKHLLQLK 1206 E++ ++ LE LK + +V+ KD +++LK + E +++ + D+K + Sbjct: 454 -ERDQLVAALEIQLKAL ISSNVQ—KDNEIEQLKRIIΞETSKIETQIMDIK PKR 504 Query: 1207 EEEEETNRQETEKLKEELSASSARTQN 1233 + ++ +TE L S + ++ Sbjct: 505 ISSADPDKLQTEPLSTSFEISRNKIED 531 Score = 186 (27.9 bits), Expect = 3.2e-10, P = 3.2e-10 Identities = 131/674 (19%), Positives = 294/674 (43%) Query: 673 LELKFNQIKAELAKTKGELIKT-KEELKKRENESDSLIQELETSNKKIITQNQRIKELIN 731 L+ K ++ + +L K K LI+ KEEL+++ D IQ + + + Q + Sbjct: 35 LKEKEHKNQDDLLKEKETLIQQLKEELQEKNVTLDVQIQHVVEGKRALSELTQGVTCYKA 94 Query: 732 IIDQKEDTINEFQNL-KSHMENTFKCNDKADTSSLIINNKLICNETVEVPKDSKSKICSE 790 I + E TI E Q + +SH + D + S+I+ + E E +DS Sbjct: 95 KIKELE-TILETQKVERSHSAKLEQ—DILEKESIILKLERNLKEFQEHLQDS VKN 147 Query: 791 RKRVNENELQ-QDEPPAKKGSIHVSSAITEDQKKSEEV-RPNIAEI-EDIRVLQENNEGL 847 K +N EL+ ++E ++ + + +++ EE R ++ E++ + L Sbjct: 148 TKDLNVKELKLKEEITQLTNNLQDMKHLLQLKEEEEETNRQETEKLKEELSASSARTQNL 207 Query: 848 RAFLLTIENELKNEKEEKAELNKQIVHFQQELSLSEKKNLTLSKEVQQI QSNYDI 902 +A L E + + KE+ + KQI Q+E+S+ ++ L ++ ++ Q + ++ Sbjct: 208 KADLQRKEEDYADLKEKLTDAKKQIKQVQKEVSVMRDEDKLLRIKINELEKKKNQCSQEL 267 Query: 903 AIAELHVQKSKNQEQEEKIMKLSNEIETATRΞITNNVSQIKLMHTKIDEL-RTLDSVSQI 961 + + +Q+ K Q +K+ + + E A + + I+ M ++E +T Q+ Sbjct: 268 DMKQRTIQQLKEQLNNQKVEEAIQQYERACKDLNVKEKIIEDMRMTLEEQEQTQVEQDQV 327 Query: 962 SNIDLLNLRDLSNGSEEDNLPNTQLDLLGNDYLVSKQVKEYRI—QEPNRENSFHSSIEA 1019 L + L+ E+ L+ N + + + N ++ S + Sbjct: 328 LEAKLEEVERLATELEKWKEKCNDLETKNNQRSNKEHENNTDVLGKLTNLQDELQESEQK 387 Query: 1020 IWEECKEIVKASSKKSHQIEELEQQIEKLQAEVKGYKDENNRLKEKEHKNQ—DDLLKEK 1077 + K+ ++ Q +E E K E+K Y ++ R +++++ + L EK Sbjct: 388 YNADRKKWLEEKMMLITQAKEAENIRNK EMKKYAEDRERFFKQQNEMEILTAQLTEK 444 Query: 1078 ETLIQQLKEELQEKNVTLDVQIQHVVEGKRALSELTQGVTCYKAKIKELETILETQKVER 1137 ++ +Q+ +EE + L++Q++ ++ + + ++ ++ET + K +R Sbjct: 445 DSDLQKWREERDQLVAALEIQLKALISSNVQKDNEIEQLKRIIΞETSKIETQIMDIKPKR 504 Query: 1138 SHSAKLEQDILEKESIILKLERNLKEFQEHLQDS VKNTKDLNVKELKLKEEITQLT 1193 SA ++ E S ++ RN E + DS +N + + +L+ + T L Sbjct: 505 ISSADPDKLQTEPLSTSFEISRNKIEDGSVVLDSCEVSTENDQSTRFPKPELEIQFTPLQ 564 Query: 1194 NNLQDMKH LLQLKEEEEETNRQETEKLKEEL-SASSARTQNLKADLQRKEEDYADLK 1249 N +KH + + + ++++ +++E+L + + + +L+ D + Sbjct: 565 PNKMAVKHPGCTTPVTVKIPKARKRKSNEMEEDLVKCENKKNATPRTNLKFPISDDRNSS 624 Query: 1250 EKLTDAKKQIKQVQKEVSVMRDEDKLLRIKINELEKKKNQCSQEL-DMKQRTIQQLKEQL 1308 K + K 1+ K+ +R + + I +N KKK Q+ D Q + L+ + Sbjct: 625 VK-KEQKVAIRPSSKKTYSLRSQASI—IGVNLATKKKEGTLQKFGDFLQHSPSILQSKA 681 Query: 1309 NNQKVEEAIQQYERACKDLNVKEKIIEDMR 1338 +K+ E + + + + + KE + + R Sbjct: 682 —KKIIETMSSSKLSNVEAS-KENVSQPKR 708 Score = 165 (24.8 bits), Expect = 5.8e-08, P = 5.8e-08 Identities = 140/626 (22%), Positives = 271/626 (43%) Query: 536 VEELENAEETQNVETKLLDEDLDKTLEENKAFISHEEKRKLLDLIEDLKKKLINEKKEK- 594 +EELE E E K +D + L+E + H+ + LL E L ++L E +EK Sbjct: 11 IEELEQQIEKLQAEVKGY-KDENNRLKEKE HKNQDDLLKEKETLIQQLKEELQEKN 65 Query: 595 LTLEFKIREEVT QEFTQYWAQREADFKE—TLLQEREILEENAERRLAIFKDLVG 647 +TL+ +1+ V E TQ +A KE T+L+ +++ E + +L +D++ Sbjct: 66 VTLDVQIQHVVEGKRALSELTQGVTCYKAKIKELETILETQKV-ERSHSAKLE—QDILE 122 Query: 648 KCDT REEAAKDICATKVETEEATACLELKFNQIKAELAKTKGELIKTKEELKKRENE 704 K E K+ ++ + T L +K ++K E+ + L K L+ +E E Sbjct: 123 KESIILKLERNLKEFQEHLQDSVKNTKDLNVKELKLKEEITQLTNNLQDMKHLLQLKEEE 182 Query: 705 SDSLIQELETSNKKIITQNQRIKELINIIDQKEDTINEFQNLKSHMENTFKCNDKADTSS 764 ++ QE E +++ + R + L + +KE+ + + + + K K + S Sbjct: 183 EETNRQETEKLKEELSAΞSARTQNLKADLQRKEEDYADLKEKLTDAKKQIKQVQK-EVSV 241 Query: 765 LIINNKLICNETVEVPKDSKSKICSERKRVNENELQQDEPPAKKGSIHVSSAITEDQKKS 824 + +KL+ + E+ K K CS+ + + +QQ + V Al + ++ Sbjct: 242 MRDEDKLLRIKINELEK—KKNQCSQELDMKQRTIQQLKEQLNNQK—VEEAIQQYERAC 297 Query: 825 EEVRPNIAEIEDIRVLQENNEGLRAFLLTIENELKNEKEEKAELNKQIVHFQQELSLSEK 884 +++ IED+R+ E E + + + L+ + EE L ++ ++++ + E Sbjct: 298 KDLNVKEKIIEDMRMTLEEQEQTQ VEQDQVLEAKLEEVERLATELEKWKEKCNDLET 354 Query: 885 KNLTLSKEVQQIQSNYDIAIAELHVQKSKNQEQEEKIMKLSNE-IETATRSITN N 938 KN S + + ++N D+ + +L + + QE E+K + +E IT N Sbjct: 355 KNNQRSNK—EHENNTDV-LGKLTNLQDELQESEQKYNADRKKWLEEKMMLITQAKEAEN 411 Query: 939 VSQIKLMHTKIDELRTLDSVSQISNIDL-LNLRD—LSNGSEEDNLPNTQLDLLGNDYLV 995 + ++ D R +++ + L +D L EE + L++ + Sbjct: 412 IRNKEMKKYAEDRERFFKQQNEMEILTAQLTEKDSDLQKWREERDQLVAALEIQLKALIS 471 Query: 996 SKQVKEYRIQEPNRENSFHSSIEA-IWE-ECKEIVKASSKKSHQIEELEQQIEKLQAEVK 1053 S K+ I++ R Ξ Ξ IE I + + K I A K Q E L E + +++ Sbjct: 472 SNVQKDNEIEQLKRIISETSKIETQIMDIKPKRISSADPDKL-QTEPLΞTSFEISRNKIE 530 Query: 1054 GYKDENNRLKEKEHKNQDDLLKEKE TLIQQLKEELQEKNVTLDVQIQHVVEGKRA 1108 + + +Q + E T +Q K ++ T V ++ KR Sbjct: 531 DGSVVLDSCEVSTENDQSTRFPKPELEIQFTPLQPNKMAVKHPGCTTPVTVKIPKARKRK 590 Query: 1109 LSELTQG-VTCYKAKIKELETILETQ-KVERSHSAKLEQDILEKES 1152 +E+ + V C K T L+ +R+ S K EQ + + S Sbjct: 591 ΞNEMEEDLVKCENKKNATPRTNLKFPISDDRNΞSVKKEQKVAIRPS 636 Score = 143 (21.5 bits), Expect = 1.3e-05, P = 1.3e-05 Identities = 164/684 (23%), Positives = 304/684 (44%) Query: 295 QKRKMLR-LSQDVKGYSFIKDLQWIQVSDSKEAYRLLKLGIKHQSVAFTKLNNASΞ 349 +K +++ L ++++ + D+Q V + K A L G+ +L Sbjct: 49 EKETLIQQLKEELQEKNVTLDVQIQHVVEGKRALSELTQGVTCYKAKIKELETILETQKV 108 Query: 350 -RSHSI-FTVKILQIEDSEMSRVIRVSELSLCDLAGSERTMKTQNEGE-RLRETGNINTS 406 RSHS IL+ E + + E L S + K N E +L+E T+ Sbjct: 109 ERSHSAKLEQDILEKESIILKLERNLKEFQE-HLQDSVKNTKDLNVKELKLKEEITQLTN 167 Query: 407 LLTLGKCINVLKNSEKSKFQQHVPFRESKLTHYFQSFFNGKGKICMIVNISQCYLAYDET 466 L K + LK E+ +Q + +L+ N K + + Y E Sbjct: 168 NLQDMKHLLQLKEEEEETNRQETEKLKEELSASSARTQNLKADL QRKEEDYADLKEK 224 Query: 467 LNVLKFSAIAQKVCVPDTLNSSQDKLFGPVKSSQDVΞLDSNSNSKILNVKRATISWENSL 526 L K I Q V ++ +DKL +K ++ + N S+ L++K+ TI Sbjct: 225 LTDAK-KQIKQ-VQKEVSVMRDEDKLLR-IKINE-LEKKKNQCSQELDMKQRTIQQLKEQ 280 Query: 527 EDLMEDEDLVEELENAEETQNVETKLLDEDLDKTLEENKAFISHEEKRKLLDL-IEDLKK 585 + + E+ +++ E A + NV+ K++ ED+ TLEE + + E+ ++L+ +E++++ Sbjct: 281 LNNQKVEEAIQQYERACKDLNVKEKII-EDMRMTLEEQEQ—TQVEQDQVLEAKLEEVER 337 Query: 586 KLIN-EK-KEKLT-LEFKIREEVTQEFTQYWAQREADFKETLLQEREILEE NAERR 638 EK KEK LE K + +E + K T LQ+ E+ E NA+R+ Sbjct: 338 LATELEKWKEKCNDLETKNNQRSNKEHEN NTDVLGKLTNLQD-ELQEΞEQKYNADRK 393 Query: 639 LAIFKDLVGKCDTREEAAKDICATKVETEEATACLELKFNQIKAELAKTKGELIKTKEEL 698 + + ++ T+ + A++I K E ++ E F Q + E+ +L + +L Sbjct: 394 KWLEEKMM—LITQAKEAENI-RNK-EMKKYAEDRERFFKQ-QNEMEILTAQLTEKDSDL 448 Query: 699 KKRENESDSLIQELETSNKKIITQN-QR IKELINIIDQKEDTINEFQNLKSHMENTF 754 +K E D L+ LE K +1+ N Q+ I++L II + + ++K ++ Sbjct: 449 QKWREERDQLVAALEIQLKALISSNVQKDNEIEQLKRIISETSKIETQIMDIKPKRISSA 508 Query: 755 KCNDKADTSSLIINNKLICN—ETVEVPKDSKSKICSERK RVNENELQ-QDEP—PA 806 DK T L + ++ N E V DS ++ +E R + EL+ Q P P Sbjct: 509 D-PDKLQTEPLSTSFEISRNKIEDGSVVLDS-CEVSTENDQSTRFPKPELEIQFTPLQPN 566 Query: 807 KKGSIH—VSSAITEDQKKSEEVRPNIAEIEDIRVLQENNEGLRA FLLTIENELKNE 861 K H ++ +T K+ + + N E + ++ + N R F ++ + + Sbjct: 567 KMAVKHPGCTTPVTVKIPKARKRKSNEMEEDLVKCENKKNATPRTNLKFPISDDRNSSVK 626 Query: 862 KEEKAEL NKQIVHFQQELSLSEKKNLTLSKEVQQIQSNYDIAIAELHVQKSKNQEQE 918 KE+K + +K+ + + S+ NL K+ +Q D + +SK ++ Sbjct: 627 KEQKVAIRPSSKKTYSLRSQASIIGV-NLATKKKEGTLQKFGDFLQHSPSILQSKAKKII 685 Query: 919 EKIM—KLSNEIETATRSITNNVSQIKLMHTKI—DELRT-LDSVSQISNID 965 E + KLSN +E + NVSQ K K+ E+ + +D Q+ +D Sbjct: 686 ETMSSSKLSN-VEASKE NVSQPKRAKRKLYTSEISΞPIDISGQVILMD 732 Score = 133 (20.0 bits), Expect = 1.6e-04, P = 1.6e-04 Identi .ties == 94/426 (22%), Positives = 188/426 (44%) Query: 527 EDLM-EDEDLVEELENAEETQNVETKLLDEDLDKTLEENKAFISHEEKRKLLDL-IEDLK 584 +DL+ E E L+++L+ + +NV LD + +E +A + I++L+ Sbjct: 44 DDLLKEKETLIQQLKEELQEKNVT LDVQIQHVVEGKRALSELTQGVTCYKAKIKELE 100 Query: 585 KKLINEKKEKLTLEFKIREEVTQ-EFTQYWAQREA-DFKETLLQEREILEENAERRLAIF 642 L +K E+ + K+ +++ + E +R +F+E L + ++ + L + Sbjct: 101 TILETQKVER-SHSAKLEQDILEKESIILKLERNLKEFQEHLQDSVKNTKDLNVKELKL- 158 Query: 643 KDLVGKCDTREEAAKDICATKVETEEATACLELKFNQIKAELAKTKGELIKTKEELKKRE 702 K+ + + + K + K E EE + ++K EL+ + K +L+++E Sbjct: 159 KEEITQLTNNLQDMKHLLQLKEEEEETN RQETEKLKEELSASSARTQNLKADLQRKE 215 Query: 703 NESDSLIQELETSNKKIITQNQRIKELINIIDQK-EDTINEFQNLKSHMENTFKCNDKA- 760 + L ++L T KK I Q Q+ ++ D+ INE + K+ + Sbjct: 216 EDYADLKEKL-TDAKKQIKQVQKEVSVMRDEDKLLRIKINELEKKKNQCSQELDMKQRTI 274 Query: 761 DTSSLIINNKLICNETVE VPKDΞ—KSKICSE-RKRVNENE LQQDEPPAKKGS 810 +NN+ + E ++ KD K KI + R + E E ++QD+ K Sbjct: 275 QQLKEQLNNQKV-EEAIQQYERACKDLNVKEKIIEDMRMTLEEQEQTQVEQDQVLEAKLE 333 Query: 811 IHVSSAITEDQKKSEEVRP-NIAEIEDIRVLQENNEGLRAFLLTIENELKNEKEEKAELN 869 V TE +K E+ + ENN + L +++EL+ E E+K + Sbjct: 334 -EVERLATELEKWKEKCNDLETKNNQRΞNKEHENNTDVLGKLTNLQDELQ-ESEQKYNAD 391 Query: 870 KQIVHFQQELSLSEKKNLTLSKEVQQIQSNYDIAIAELHVQKSKNQEQEEKIMKLSNEIE 929 ++ ++++ L +T +KE + I++ + K E E+ K NE+E Sbjct: 392 RK-KWLEEKMML ITQAKEAENIRNK EMKKYAEDRERFFKQQNEME 435 Query: 930 TATRSITNNVSQIKLMHTKIDEL 952 T +T S ++ + D+L Sbjct: 436 ILTAQLTEKDSDLQKWREERDQL 458 Pedant information for DKFZphtes3_35b4, frame 3 Report for DKFZphtes3_35b4.3 [LENGTH] 1780 [MW] 206176.77 [pi] 5.60 [HOMOL] TREMBL :U93121_1 product: "M-phase phosphoproteιn-1"; Human M-phase phosphoproteιn-1 mRNA, partial eds. 0.0 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YEL061c] 2e-37 [FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YEL061C] 2e-37 [FUNCAT] 08.22 cytoskeleton-dependent transport [S. cerevisiae, YELOδlc] 2e-37 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YELOδlc] 2e-37 [FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YDL058w] 7e-30 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YDL058w] 7e-30 [FUNCAT] 30.05 organization of centrosome [S. cerevisiae, YPR141c] 3e-23 [FUNCAT] 11.01 stress response [S. cerevisiae, YPR141c] 3e-23 [FUNCAT] 03.07 pheromone response, matmg-type determination, sex-specific proteins [S. cerevisiae, YPR141c] 3e-23 [FUNCAT] 03.13 meiosis [S. cerevisiae, YPR141c] 3e-23 [FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YPR141c] 3e-23 [FUNCAT] 09.10 nuclear biogenesis [S. cerevisiae, YPR141C] 3e-23 [FUNCAT] 11.04 dna repair (direct repair, base excision repair and nucleotide excision repair) [S. cerevisiae, YKR095w] le-21 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YLR309c] 6e-20 [FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YHR023w MYOl - myosιn-1 isoform] 4e-19 [FUNCAT] 03.25 cytokinesis [S. cerevisiae, YHR023w MYOl - myosιn-1 isoform] 4e-19 [FUNCAT] 03.19 recombination and dna repair [S. cerevisiae, YNL250w] le-15 [FUNCAT] 1 genome replication, transcription, recombination and repair [M. jannaschii MJ1322] 2e-14 [FUNCAT] 30.13 organization of chromosome structure [Ξ. cerevisiae, YDR285w] 2e-09 [FUNCAT] 09.04 biogenesis of cytoskeleton [S. cerevisiae, YKL179c] 3e-09 [FUNCAT] 09.13 biogenesis of chromosome structure [S. cerevisiae, YLR086w] 2e-07 [FUNCAT] 03.01 cell growth [S. cerevisiae, YNL079c] 2e-07 [FUNCAT] 08.99 other intracellular-transport activities [S. cerevisiae, YNL079c] 2e-07 [FUNCAT] 03.22.01 cell cycle check point proteins [S. cerevisiae, YGL086w] le-06 [FUNCAT] 10.05.99 other pheromone response activities [S. cerevisiae, YHR158c] 3e-06 [FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YDR217c] 4e-06 [FUNCAT] 98 classification not yet clear-cut [S. cerevisiae, YJR134c] 2e-05 [FUNCAT] 05.04 translation (initiation, elongation and termination) [S. cerevisiae, YAL035W] 2e-04 [FUNCAT] r general function prediction [M. jannaschii, MJ1254] 0.001 [BLOCKS] BL00387A [BLOCKS] BL00411H [BLOCKS] BL00411G [BLOCKS] BL00411F [BLOCKS] BL00411E Kinesin motor domain proteins [BLOCKS] BL00411D Kinesin motor domain proteins [BLOCKS] BL00411C Kinesin motor domain proteins [BLOCKS] BL00411B Kinesm motor domain proteins [BLOCKS] BL00411A Kmesin motor domain proteins [SCOP] d2kιn.l 3.29.1.5.3 Kmesin [Rat (Rattus norvegicus) 2e-68 [SCOP] d2tmab_ 1.105.4.1.1 Tropomyosin [rabbit (Oryetolagus cumeulus) 4e-05 [SCOP] d3kar 3.29.1.5.4 Kinesin [Baker's yeast (Saccharomyce 2e-09 [EC] 3.6.1.32 Myosin ATPase 5e-25 [PIRKW] nucleus 4e-27 [PIRKW] phosphotransferase 3e-16 [PIRKW] duplication 6e-20 [PIRKW] c trulline 6e-18 [PIRKW] tandem repeat 4e-24 [PIRKW] heterodimer 3e-28 [PIRKW] endocytosis le-23 [PIRKW] heart le-17 [PIRKW] transmembrane protein 2e-28 [PIRKW] serme/threo ne-specific protein kinase 3e-16 [PIRKW] zmc fmger le-23 [PIRKW] surface antigen 2e-16 [PIRKW] DNA binding le-25 [PIRKW] metal binding le-23 [PIRKW] muscle contraction 4e-24 [PIRKW] heterotetramer 4e-24 [PIRKW] acetylated amino end 2e-19 [PIRKW] actin binding 5e-25 [PIRKW] mitosis 3e-58 [PIRKW] microtubule binding 3e-58 [PIRKW] ATP 3e-58 [PIRKW] thick filament 4e-24 [PIRKW] phosphoprotein 9e-29 [PIRKW] leucine zipper le-12 [PIRKW] skeletal muscle 8e-24 [PIRKW] disulfide bond le-12 [PIRKW] heterotrimer le-29 [PIRKW] calcium binding 6e-18 [PIRKW] alternative splicing 4e-21 [PIRKW] P-loop 2e-63 [PIRKW] coiled coil 3e-58 [PIRKW] heptad repeat le-25 [PIRKW] methylated ammo acid 4e-24 [PIRKW] peripheral membrane protein le-23 [PIRKW] dimer le-12 [PIRKW] cardiac muscle le-17 [PIRKW] hydrolase 5e-25 [PIRKW] microtubule 6e-15 [PIRKW] muscle 7e-23 [PIRKW] membrane protein 6e-20 [PIRKW] GTP binding 8e-22 [PIRKW] EF hand 6e-18 [PIRKW] cell division le-25 [PIRKW] cytoskeleton 4e-24 [PIRKW] hair 6e-18 [PIRKW] Golgi apparatus 8e-24 [PIRKW] calmodulin binding le- 2 3 [SUPFAM] unassigned Ser/Thr or Tyr-specific protein kinases 3e-16 [SUPFAM] myosin motor domain homology 5e-25 [SUPFAM] alpha-actinin actin-bmding domain homology le-13 [SUPFAM] kinesin-related protein KIP1 9e-27 [SUPFAM] kmesin-related protein CIN8 4e-36 [SUPFAM] kinesin heavy chain 4e-24 [SUPFAM] plectm le-13 [SUPFAM] trichohyalm 6e-18 [SUPFAM] kinesm-related protein KIF3 le-29 [SUPFAM] kinesm-related protein KIF2 3e-20 [SUPFAM] ribosomal protein S10 homology le-13 [SUPFAM] giantin 8e-24 [SUPFAM] protein kinase homology 3e-16 [SUPFAM] protein kinase C zinc-bmding repeat homology 2e-13 [SUPFAM] kinesin-related protein unc-104 8e-26 [SUPFAM] human early endosome antigen 1 le-23 [SUPFAM] unassigned kmesin-related proteins le-28 [SUPFAM] Mycoplasma ge talium hypothetical protein MG218 4e-17 [SUPFAM] myosin heavy chain 5e-25 [SUPFAM] conserved hypothetical P115 protein 4e-20 [SUPFAM] centromere protein E 5e-24 [SUPFAM] calmodulin repeat homology 6e-18 [SUPFAM] kinesin-related protein KLP61F le-25 [SUPFAM] hypothetical protein MJ0914 3e-12 [SUPFAM] kinesin-related protein MKLP-1 2e-63 [SUPFAM] pleckstrm repeat homology 8e-26 [SUPFAM] hypothetical protein MJ1322 4e-13 [SUPFAM] kinesm-related protein KIF1B 3e-28 [SUPFAM] kinesin motor domain homology 2e-63 [SUPFAM] k esin-related protein KLPA 7e-25 [SUPFAM] kinesin-related protein nodA le-12 [SUPFAM] kinesin-related protein Eg5 5e-30 [PROSITE] ATP_GTP_A 1 [PFAM] Kinesin motor domain [KW] Irregular [KW] 3D [KW] LOW_COMPLEXITY 7.53 % [KW] COILED_COIL 19.78 % SEQ MESNFNQEGVPRPSYVFSADPIARPSEINFDGIKLDLSHEFSLVAPNTEANSFESKDYLQ SEG COILS 3kar- SEQ VCLRIRPFTQSEKELESEGCVHILDSQTVVLKEPQCILGRLSEKSSGQMAQKFSFSKVFG SEG COILS 3kar- SEQ PATTQKEFFQGCIMQPVKDLLKGQSRLIFTYGLTNSGKTYTFQGTEENIGILPRTLNVLF SEG COILS 3kar- SEQ DSLQERLYTKMNLKPHRSREYLRLSSEQEKEEIASKSALLRQIKEVTVHNDSDDTLYGSL SEG COILS 3kar- SEQ TNSLNISEFEESIKDYEQANLNMANSIKFSVWVSFFEIYNEYIYDLFVPVSSKFQKRKML SEG COILS 3kar- EEEEEEEEEEETTEEEETTTCC CCEE SEQ RLSQDVKGYSFIKDLQWIQVSDSKEAYRLLKLGIKHQSVAFTKLNNASSRSHSIFTVKIL SEG COILS 3kar- EEETTTTE-EEEETTCCEEECCGGGHHHHHHHHHHHHCCTTTTCHHHHHHCEEEEEEEEE SEQ QIEDΞEMSRVIRVSELSLCDLAGSERTMKTQNEGERLRETGNINTSLLTLGKCINVLKNS SEG COILS 3kar- E—EETTTTCEEEEEEEEEECCCCCCC CCCHHHHHHHHHHHHHHHHHHHHHHHHTT SEQ EKSKFQQHVPFRESKLTHYFQSFFNGKGKICMIVNISQCYLAYDETLNVLKFSAIAQKVC SEG COILS 3kar- TTTT—TCCTTTTTHHHHHHGGGCTTTTEEEEEEEECCCGGGHHHHHHHHHHHH SEQ VPDTLNSSQDKLFGPVKΞSQDVSLDSNSNSKILNVKRATISWENSLEDLMEDEDLVEELE SEG xxxxxxxxxxxxxxxxxx COILS 3kar- SEQ NAEETQNVETKLLDEDLDKTLEENKAFISHEEKRKLLDLIEDLKKKLINEKKEKLTLEFK SEG xxxxxxxxxxxxxxxxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxxxxxxx .. COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC 3kar- SEQ IREEVTQEFTQYWAQREADFKETLLQEREILEENAERRLAIFKDLVGKCDTREEAAKDIC SEG COILS CCCCCCC 3kar- SEQ ATKVETEEATACLELKFNQIKAELAKTKGELIKTKEELKKRENESDSLIQELETSNKKII SEG COILS ccccccccccccccc 3kar- SEQ TQNQRIKELINIIDQKEDTINEFQNLKSHMENTFKCNDKADTSSLIINNKLICNETVEVP SEG COILS CCCCCCCCCCCCCCC 3kar- SEQ KDSKSKICSERKRVNENELQQDEPPAKKGSIHVSSAITEDQKKSEEVRPNIAEIEDIRVL SEG COILS CCCC 3kar- SEQ QENNEGLRAFLLTIENELKNEKEEKAELNKQIVHFQQELSLSEKKNLTLSKEVQQIQSNY SEG xxxxxxxxxxxxxxxx COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC 3kar- SEQ DIAIAELHVQKSKNQEQEEKIMKLSNEIETATRSITNNVSQIKLMHTKIDELRTLDSVΞQ SEG COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC 3kar- SEQ ISNIDLLNLRDLSNGSEEDNLPNTQLDLLGNDYLVSKQVKEYRIQEPNRENSFHSSIEAI SEG COILS 3kar- SEQ WEECKEIVKASSKKSHQIEELEQQIEKLQAEVKGYKDENNRLKEKEHKNQDDLLKEKETL SEG xxxxxxxxxxxxx COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC 3kar- SEQ IQQLKEELQEKNVTLDVQIQHVVEGKRALSELTQGVTCYKAKIKELETILETQKVERSHS SEG COILS ccccccccccccccccccccccccccccccccc . . . . 3kar- SEQ AKLEQDILEKESIILKLERNLKEFQEHLQDΞVKNTKDLNVKELKLKEEITQLTNNLQDMK SEG COILS cccccccccccccccccccccccccccccc ccccccccccccccccccccccccc 3kar- SEQ HLLQLKEEEEETNRQETEKLKEELSASΞARTQNLKADLQRKEEDYADLKEKLTDAKKQIK SEG . xxxxxxxxxxxxxxxxxxx COILS ccccc cccccccccccccccccccccccccccccccc 3kar- SEQ QVQKEVSVMRDEDKLLRIKINELEKKKNQCSQELDMKQRTIQQLKEQLNNQKVEEAIQQY SEG COILS cccccccccccc 3kar- SEQ ERACKDLNVKEKIIEDMRMTLEEQEQTQVEQDQVLEAKLEEVERLATELEKWKEKCNDLE SEG xxxxxxxxxxxxxxxxx COILS CCCCCCCCCCCCCCCCCCCCCCCCCC 3kar- SEQ TKNNQRSNKEHENNTDVLGKLTNLQDELQESEQKYNADRKKWLEEKMMLITQAKEAENIR SEG COILS CC 3kar- SEQ NKEMKKYAEDRERFFKQQNEMEILTAQLTEKDΞDLQKWREERDQLVAALEIQLKALISSN SEG COILS 3kar- SEQ VQKDNEIEQLKRIISETSKIETQIMDIKPKRISSADPDKLQTEPLSTSFEISRNKIEDGS SEG COILS 3kar- SEQ VVLDSCEVSTENDQSTRFPKPELEIQFTPLQPNKMAVKHPGCTTPVTVEIPKARKRKSNE SEG COILS 3kar- SEQ MEEDLVKCENKKNATPRTNLKFPISDDRNSSVKKEQKVAIRPSSKKTYSLRSQAΞIIGVN SEG COILS 3kar- SEQ LATKKKEGTLQKFGDFLQHSPSILQSKAKKIIETMSΞSKLSNVEASKENVSQPKRAKRKL SEG COILS 3kar- SEQ YTSEISSPIDISGQVILMDQKMKESDHQIIKRRLRTKTAK SEG COILS 3kar- Prosite for DKFZphtes3_35b4.3 PS00017 152->160 ATP GTP A PDOC00017 Pfam for DKFZphtes3_35b4.3 HMM_NAME Kinesin motor domain HMM *RCRPlNeREιndgcscvVQWPpWtGyktvhnghegds phks R+RP+ + E++ + +V + ++++ ++ + ++ Query 64 RIRPFTQSEKELESEGCVHILDSQTVVLKEPQCILGRLSEKSSGQMAQK 112 HMM FtFDHVFWWncTQedVYdtvAHPIVDDcFhGYNCTIFAYGQTGSGKTYTM F+F +VF++++TQ++ +++ + V+D+++G IF+YG T SGKTYT Query 113 FSFSKVFGPATTQKEFFQGCIMQPVKDLLKGQSRLIFTYGLTNSGKTYTF 162 HMM MGpggehPDHmGIIPRcCHDIFdrldkfqekDhdFW G +++GI+PR+++ +FD++ + +++ Query 163 QG TEENIGILPRTLNVLFDSLQERL-YTKMNLKPHRSREYLRLSSE 207 HMM Query 208 QEKEEIASKSALLRQIKEVTVHNDSDDTLYGSLTNSLNISEFEESIKDYE 257 HMM hVkCSYMEIYNEelYDLLCPnP... qhMkpLnlHEHPN +V +S++EIYNE+IYDL +P++ Q++K L++ + + Query 258 QANLNMANSIKFSVWVSFFEIYNEYIYDLFVPVSSKFQKRKMLRLSQDVK 307 HMM MGpYVqGCTEfHVcSYeDachWIWqGnknRHVAaTnMNdhSSRSHtlFTI ++++++ V +A +++ +G K+ VA T++N SSRSH+IFT+ Query 308 GYSFIKDLQWIQVSDSKEAYRLLKLGIKHQSVAFTKLNNASSRSHSIFTV 357 HMM HVeQrHk . qcdehvcHSKMNLVDLAGSERvnrTGAEGQRIKEGcNINqSL ++ Q + + +++S ++L DLAGΞER+ +T+ EG RL+E +NIN SL Query 358 KILQIEDSEMSRVIRVSELSLCDLAGSERTMKTQNEGERLRETGNINTSL 407 HMM ttLGnVInaLaDgqTKYmYgghgHIPYRDSKLTWlLQDSLGGNcKTcMIA +TLG++IN+L + + + +H+P+R+SKLT+ +Q + G +K CMI+ Query 408 LTLGKCINVLKNSE KSKFQQHVPFRESKLTHYFQSFFNGKGKICMIV 454 HMM CIWPadWNYEETLSTLRYAdRAKnlkNkPQINEDPca* +1+ + Y+ETL++L++ + A+++ + ++N+++++ Query 455 NISQCYLAYDETLNVLKFSAIAQKVCVPDTLNSSQDK 491 DKFZphtes3_35b5 group: metabolism DKFZphtes3_35b5 encodes a novel 466 ammo acid protein, with similarity to bovme accessory subunit for vacuolar ATPase and rat C7-1 protein. The vacuolar proton-ATPase (V-ATPase) translocates protons into intracellular organelles or across the plasma membrane of specialized cells. The catalytic domain consists of a hexamer of 3 A subunits and 3 B subunits, plus accessory subunits C, D, and E. The rat homolog C7-1 seems to be enriched in aged adult rats in the frontal cortex. The novel protein can find application in modulating the v-ATPase activity in endocytic and secretory organelles. strong similarity to bovine vacuolar ATPase (EC 3.6.1.-) chain A complete cDNA, complete eds potential start at Bp 8 , EST hits matches perfect to 154197 hypothetical protein, but posess 186 aa additional at N-termmus Sequenced by DKFZ Locus : unknown Insert length: 2043 bp Poly A stretch at pos. 2033, polyadenylation signal at pos. 2012 1 GGCGGCCATG GCGACGGCTC GAGTGCGGAT GGGGCCGCGG TGCGCCCAGG 51 CGCTCTGGCG CATGCCGTGG CTGCCGGTGT TTTTGTCGTT GGCGGCGGCG 101 GCGGCGGCGG CAGCGGCGGA GCAGCAGGTC CCGCTGGTGC TGTGGTCGAG 151 TGACCGGGAC TTGTGGGCTC CTGCGGCCGA CACTCATGAA GGCCACATCA 201 CCAGCGACTT GCAGCTCTCT ACCTACTTAG ATCCCGCCCT GGAGCTGGGT 251 CCCAGGAATG TGCTGCTGTT CCTGCAGGAC AAGCTGAGCA TTGAGGATTT 301 CACAGCATAT GGCGGTGTGT TTGGAAACAA GCAGGACAGC GCCTTTTCTA 351 ACCTAGAGAA TGCCCTGGAC CTGGCCCCCT CCTCACTGGT GCTTCCTGCC 401 GTCGACTGGT ATGCAGTCAG CACTCTGACC ACTTACCTGC AGGAGAAGCT 451 CGGGGCCAGC CCCTTGCATG TGGACCTGGC CACCCTGCGG GAGCTGAAGC 501 TCAATGCCAG CCTCCCTGCT CTGCTGCTCA TTCGCCTGCC CTACACAGCC 551 AGCTCTGGTC TGATGGCACC CAGGGAAGTC CTCACAGGCA ACGATGAGGT 601 CATCGGGCAG GTCCTGAGCA CACTCAAGTC CGAAGATGTC CCATACACAG 651 CGGCCCTCAC AGCGGTCCGC CCTTCCAGGG TGGCCCGTGA TGTAGCCGTG 701 GTGGCCGGAG GGCTAGGTCG CCAGCTGCTA CAAAAACAGC CAGTATCACC 751 TGTGATCCAT CCTCCTGTGA GTTACAATGA CACCGCTCCC CGGATCCTGT 801 TCTGGGCCCA AAACTTCTCT GTGGCGTACA AGGACCAGTG GGAGGACCTG 851 ACTCCCCTCA CCTTTGGGGT GCAGGAACTC AACCTGACTG GCTCCTTCTG 901 GAATGACTCC TTTGCCAGGC TCTCACTGAC CTATGAACGA CTCTTTGGTA 951 CCACAGTGAC ATTCAAGTTC ATTCTGGCCA ACCGCCTCTA CCCAGTGTCT 1001 GCCCGGCACT GGTTTACCAT GGAGCGCCTC GAAGTCCACA GCAATGGCTC 1051 CGTCGCCTAC TTCAATGCTT CCCAGGTCAC AGGGCCCAGC ATCTACTCCT 1101 TCCACTGCGA GTATGTCAGC AGCCTGAGCA AGAAGGGTAG TCTCCTCGTG 1151 GCCCGCACGC AGCCCTCTCC CTGGCAGATG ATGCTTCAGG ACTTCCAGAT 1201 CCAGGCTTTC AACGTAATGG GGGAGCAGTT CTCCTACGCC AGCGACTGTG 1251 CCAGCTTCTT CTCCCCCGGC ATCTGGATGG GGCTGCTCAC CTCCCTGTTC 1301 ATGCTCTTCA TCTTCACCTA TGGCCTGCAC ATGATCCTCA GCCTCAAGAC 1351 CATGGATCGC TTTGATGACC ACAAGGGCCC CACTATTTCT TTGACCCAGA 1401 TTGTGTGACC CTGTGCCAGT GGGGGGGTTG AGGGTGGGAC GGTGTCCGTG 1451 TTGTTGCTTT CCCACCCTGC AGCGCACTGG ACTGAAGAGC TTCCCTCTTC 1501 CTACTGCAGC ATGAACTGCA AGCTCCCCTC AGCCCATCTT GCTCCCTCTT 1551 CAGCCCGCTG AGGAGCTTTC TTGGGCTGCC CCCATCTCTC CCAACAAGGT 1601 GTACATATTC TGCGTAGATG CTAGACCAAC CAGCTTCCCA GGGTTCGTCG 1651 CTGTGAGGCG TAAGGGACAT GAATTCTAGG GTCTCCTTTC TCCTTATTTA 1701 TTCTTGTGGC TACATCATCC CTGGCTGTGG ATAGTGCTTT TGTGTAGCAA 1751 ATGCTCCCTC CTTAAGGTTA TAGGGCTCCC TGAGTTTGGG AGTGTGGAAG 1801 TACTACTTAA CTGTCTGTCC TGCTTGGCTG CCGTTATCGT TTTCTGGTGA 1851 TGTTGTGCTA ACAATAAGAA GTACACGGGT TTATTTCTGT GGCCTGAGAA 1901 GGAAGGGACC TCCACGACAG GTGGGCTGGG TGCGATCGCC GGCTGTTTGG 1951 CATGTTCCCA CCGGGAGTGC CGGGCAGGAG CATGGGGTGC TTGGTTGTTT 2001 CCTTCCTAAT AAAATAAACG CGGGTCGCCA TGCAAAAAAA AAA BLAST Results No BLAST result Medline entries 95014142: A novel accessory subunit for vacuolar H(+) -ATPase from chromaffm granules . 97215246: Identification of a rat brain gene associated with aging by PCR differential display method. Peptide information for frame 2 ORF from 8 bp to 1405 bp; peptide length: 466 Category: strong similarity to known protein 1 MATARVRMGP RCAQALWRMP WLPVFLSLAA AAAAAAAEQQ VPLVLWSSDR 51 DLWAPAADTH EGHITSDLQL STYLDPALEL GPRNVLLFLQ DKLSIEDFTA 101 YGGVFGNKQD SAFSNLENAL DLAPSSLVLP AVDWYAVSTL TTYLQEKLGA 151 SPLHVDLATL RELKLNASLP ALLLIRLPYT ASSGLMAPRE VLTGNDEVIG 201 QVLSTLKSED VPYTAALTAV RPSRVARDVA VVAGGLGRQL LQKQPVSPVI 251 HPPVSYNDTA PRILFWAQNF SVAYKDQWED LTPLTFGVQE LNLTGSFWND 301 SFARLSLTYE RLFGTTVTFK FILANRLYPV SARHWFTMER LEVHSNGSVA 351 YFNASQVTGP SIYSFHCEYV SSLSKKGSLL VARTQPSPWQ MMLQDFQIQA 401 FNVMGEQFSY ASDCASFFSP GIWMGLLTSL FMLFIFTYGL HMILSLKTMD 451 RFDDHKGPTI SLTQIV BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_35b5, frame 2 TREMBL:AF035387_1 gene: "C7-1"; product: "C7-1 protein"; Rattus norvegicus C7-1 protein (C7-1) mRNA, complete eds., N = 1, Score = 2088, P = 3.8e-216 PIR:A55116 vacuolar ATPase (EC 3.6.1.-) chain Ac45 - bovme, N = 1, Score = 2011, P = 5.5e-208 PIR:I54197 hypothetical protein - human, N = 1, Score = 1464, P = 5.1e-150 >TREMBL:AF035387_1 gene: "C7-1"; product: "C7-1 protein"; Rattus norvegicus C7-1 protein (C7-1) mRNA, complete eds. Length = 463 HSPs: Score = 2088 (313.3 bits), Expect = 3.8e-216, P = 3.8e-216 Identities = 408/463 (88%), Positives = 426/463 (92%) Query: 4 ARVRMGPRCAQALWRMPWLPVFLSLAAAAAAAAAEQQVPLVLWSSDRDLWAPAADTHEGH 63 +R+R G R A LW + LSL A AAA AAEQQVPLVLWSSDRDLWAP ADTHEGH Sbjct: 8 SRIRTGTRWAPVLW LLLSLVAVAAAVAAEQQVPLVLWSSDRDLWAPVADTHEGH 61 Query: 64 ITSDLQLSTYLDPALELGPRNVLLFLQDKLSIEDFTAYGGVFGNKQDSAFSNLENALDLA 123 ITSD+QLSTYLDPALELGPRNVLLFLQDKLSIEDFTAYGGVFGNKQDSAFSNLENALDLA Sbjct: 62 ITSDMQLSTYLDPALELGPRNVLLFLQDKLΞIEDFTAYGGVFGNKQDSAFSNLENALDLA 121 Query: 124 PSSLVLPAVDWYAVSTLTTYLQEKLGASPLHVDLATLRELKLNASLPALLLIRLPYTASS 183 PSSLVLPAVDWYA+STLTTYLQEKLGASPLHVDLATL+ELKLNASLPALLLIRLPYTASS Sbjct: 122 PSSLVLPAVDWYAISTLTTYLQEKLGASPLHVDLATLKELKLNASLPALLLIRLPYTASS 181 Query: 184 GLMAPREVLTGNDEVIGQVLSTLKSEDVPYTAALTAVRPΞRVARDVAVVAGGLGRQLLQK 243 GLMAPREVLTGNDEVIGQVLSTL+SEDVPYTAALTAVRPSRVARDVA+VAGGLGRQLLQ Sbjct: 182 GLMAPREVLTGNDEVIGQVLSTLESEDVPYTAALTAVRPSRVARDVAMVAGGLGRQLLQT 241 Query: 244 QPVSPVIHPPVSYNDTAPRILFWAQNFSVAYKDQWEDLTPLTFGVQELNLTGSFWNDSFA 303 Q SP IHPPVΞYNDTAPRILFWAQNFSVAYKD+W+DLT LTFGV+ LNLTGSFWNDSFA Sbjct: 242 QVASPAIHPPVSYNDTAPRILFWAQNFSVAYKDEWKDLTSLTFGVENLNLTGSFWNDSFA 301 Query: 304 RLSLTYERLFGTTVTFKFILANRLYPVSARHWFTMERLEVHSNGSVAYFNASQVTGPSIY 363 LSLTYE LFG TVTFKFILA+R YPVSAR+WFTMERLE+HSNGSVA+FN ΞQVTGPSIY Sbjct: 302 MLSLTYEPLFGATVTFKFILASRFYPVSARYWFTMERLEIHSNGSVAHFNVSQVTGPSIY 361 Query: 364 SFHCEYVSSLSKKGSLLVARTQPSPWQMMLQDFQIQAFNVMGEQFSYASDCASFFSPGIW 423 SFHCEYVSSLSKKGSLLV PS WQM L +FQIQAFNV GEQFSYASDCA FFSPGIW Sbjct: 362 SFHCEYVSSLSKKGSLLVTNV-PSLWQMTLHNFQIQAFNVTGEQFΞYASDCAGFFSPGIW 420 Query: 424 MGLLTSLFMLFIFTYGLHMILSLKTMDRFDDHKGPTISLTQIV 466 MGLLT+LFMLFIFTYGLHMILSLKTMDRFDD KGPTI+LTQIV Sbjct: 421 MGLLTTLFMLFIFTYGLHMILSLKTMDRFDDRKGPTITLTQIV 463 Pedant information for DKFZphtes3_35b5, frame 2 Report for DKFZphtes3_35b5.2 [LENGTH] 466 [MW] 51621.44 [pi] 5.73 [HOMOL] TREMBL:AF035387 1 gene: "C7-1 product: "C7-1 protein"; Rattus norvegicus C7-1 protein (C7-1) mRNA, complete eds ;. 0.0 [PIRKW] hydrolase 0.0 [PROSITE] MYRISTYL 7 [PROSITE] CAMP PHOSPHO SITE 1 [PROSITE] CK2 PHOSPHO SITE 7 [PROSITE] TYR PHOSPHO SITE 1 [PROSITE] PKC PHOSPHO SITE 8 [PROSITE] ASN GLYCOSYLATION 7 [KW] SIGNAL PEPTIDE 38 [KW] TRANSMEMBRANE 1 [KW] LOW COMPLEXITY 11.59 % SEQ MATARVRMGPRCAQALWRMPWLPVFLSLAAAAAAAAAEQQVPLVLWSSDRDLWAPAADTH SEG xxxxxxxxx PRD ccceeeecccchhhhhhhcccchhhhhhhhhhhhhhhhhccceeeecccccccccccccc MEM SEQ EGHITSDLQLSTYLDPALELGPRNVLLFLQDKLSIEDFTAYGGVFGNKQDSAFSNLENAL SEG PRD ccccccchhhhhccccccccccccceeecccccccccccccccccccccchhhhhhhhcc MEM SEQ DLAPSSLVLPAVDWYAVSTLTTYLQEKLGASPLHVDLATLRELKLNASLPALLLIRLPYT SEG xxxxxxxxxxxxxxx ... PRD ccccccccccccceeeeehhhhhhhhhhccccchhhhhhhhhhhhhhcchhhhhhhcccc MEM SEQ ASSGLMAPREVLTGNDEVIGQVLSTLKSEDVPYTAALTAVRPSRVARDVAVVAGGLGRQL SEG xxxxxxxxxxxxxxxxxxxx .. PRD cccccceeeeeecccccchhhhhhhccccccchhhhhhhccccceeehhhhhccccchhh MEM SEQ LQKQPVSPVIHPPVSYNDTAPRILFWAQNFSVAYKDQWEDLTPLTFGVQELNLTGSFWND SEG PRD hhhhccccccccccccccccceeeeeccccceeeeccccccccceeeeeecccccccccc MEM SEQ SFARLSLTYERLFGTTVTFKFILANRLYPVSARHWFTMERLEVHSNGSVAYFNASQVTGP SEG PRD hhhhhhhhhhhhccceeeeeeecccccccccchhhhhhhhhhcccccceeeeeecccccc MEM SEQ SIYSFHCEYVSSLSKKGSLLVARTQPSPWQMMLQDFQIQAFNVMGEQFSYASDCASFFSP SEG xxxxxxxxxx PRD ceeeeeeeeeeecccccceeeeeccccchhhhhhhhheeeeccccccccccccccccccc MEM MMMMMM SEQ GIWMGLLTSLFMLFIFTYGLHMILΞLKTMDRFDDHKGPTISLTQIV SEG PRD ccchhhhhhhhhhhhhhhhhhhhhhhhhhhccccccccceeeeccc MEM MMMMMMMMMMMMMMMMMMMMMMM Prosite for DKFZphtes3_35b5.2 PS00001 166->170 ASN_GLYCOSYLATION PDOC00001 PS00001 257->261 AΞN_GLYCOSYLATION PDOC00001 PS00001 269->273 ASN GLYCOSYLATION PDOC00001 SO o o CO H U α. SO o oooooooooooooooooooooooooooo o oooooooooooooooooooooooooooo oooooooooooooooooooooooooooo oooooooooooooooooooooooooooo o DKFZphtes3_35e21 group: differentiation/development DKFZphtes3_35e21.2 encodes a novel 104 ammo acid putative interleukin precursor, related to mterleukm-7. Due to the close relationship to human ιnterleukιn-7, the novel interleukin is expected to act as a new growth factor for human B lineage cells. Additionally, the protein should induce the gene rearrangement of the T-cell receptor repertoire, leading to thymocyte commitment, and subsequently induce both cytotoxic T-cell- and lymphocyte-activated killer cells. This new interleukin could find clinical application in a variety of conditions of hematoly phopoietic failure and different tumours, because of its recruitment of B cell lineage cells, cytotoxic T-cell- and lymphocyte-activated killer cells. similarity to mterleukιn-7 precursor complete cDNA, complete eds, EST hits Sequenced by DKFZ Locus: unknown Insert length: 2095 bp Poly A stretch at pos. 2085, polyadenylation signal at pos. 2067 1 GGATGAAAGT GATTTAATTC ATTTTTAGAA TTTTTTTTTT GTTTTGTTTT 51 AGCAACATGC TGAACAACTA ATTTACTTTA AAAATAAGCC AGTTAAAACA 101 AAGGACGCTA AGCCCAAGTG GGGGGCAATA TTAGTCAGGA TCTTTGGGGT 151 CTAATTCCAG ACCAACTTTC AGAAGCACTT CTTTGTCTCT GTTCTCACCT 201 CTGCTGTCCC TCTCTTCCCT CATCCCCTAA GAGAGACAAA GATAAAAGCC 251 CACCTGCATC CCTAAGTCTT ACTGAGATCA GCCACCCCAG GGGAGAGAAA 301 CTGGATCTAC TTACAGCCAC CCCCTGTTTC CATCCATATA CTTACTTCCC 351 CCAATTTGCA TGTGATTATG GAAACAAGTC ATGCTCATGA AAGCAACTGT 401 AAAATAAAAG GTTATGGAGT AGTTCAGCAA CTTCTTCACA GCCAGCTTTG 451 TGGAGCTGGG GAGGACTTAG GGCCCATTGG AGTCTCTTAT GTGTACAGCT 501 TCAGGGCTGT CCCTTTCAGT TTGATTTTAA GCAATGCCTC ACTTCATAGC 551 TTAGGGGGTA AGGATTCCAT TCAGGTAGGT TGTCTAAAGG AACTAATGGG 601 ACCTCTCAGT GAATTAGCTG ACCAGATTTT AGGAAATCTT TTTAATTTCT 651 ATGATTTTCC TTCTCACATT TTGAAATGGT AAAATTGACT GGAAATAATT 701 TTTCTTGGTG CCTTATTGGT TTTCCTTGCA AACCTTTCTC ATATTTTCTC 751 ATGACCATTG CCAGTGACCA AGGCCCATGT GTGTGTTGTG TGTAATTGTG 801 GGCATGTACA AGCTTAAATA ACGTGCCGAC AGCACTGTTT CAAAGTTGGT 851 ATTCATTAGG CTGTTGCCTC CTGGGCTGGA GCTGCGCTAA TCCTGACACC 901 GGCTGCCAGG AGAAAACCTC ATGGATCACA CACCAAACCT TAATAACAGC 951 ATCCGTGACC TGCACTCTCC AGTACAGAAT GGGAACCCCA GAGCTAGGAA 1001 ATGTAGTTGT ATATTTTAAT GAACTGCTAC CCCAGCCAAA GAAGCTTCTT 1051 TCACTTTTGT GCTCTACAGA AAGCCCAAGG GGGGTAGGAG GGACAGAGCT 1101 TTGAATAACT GCTTTCTAAC ACTAAATGTG GCCAACAGGA CAGAGCACAT 1151 CACACGTATA GGCAGGTGTG AGGGACAGTG GCTAAGAATT GCCTGCTCCC 1201 TCTGCATGCT CTTTCTTGTT TCCAAAGTCC AATCAAGTGA TCCTGGGAAA 1251 CAAATCTGTC TGGATTGCGG AGGGTGGTTC TGAAAGAACT GCCAAGACGT 1301 TAAAGAAGGG TGAAGAGTAG GCAGAATATA AGTAGCTAAC CTGAGTCAAG 1351 ACTCTCAAAA GCTAGCAGCC TGATGACAAT AGGATTTATT TCAGCCAGGA 1401 TAGTGTCTGT CTGTGAGTGC ATCATTTTAA GACAGTATGA CTTCATGTTG 1451 TTACAAACTA TGTATAGTAT GTATGTTTTG TGGGTTGTAT ATATACATAA 1501 TATATATTAT ATATATATAT GAGAGATTTG GTGACTTTTG ATACGGGTTT 1551 GGTGCAGGTG AATTTATTAC TGAGCCAAAT GAGGCACATA CCGAGTCAGT 1601 AGTTGAAGTC CAGGGCATTC GATACTGTTT ATGATTTCCA TATATGTATA 1651 GTGCCTATCC CATGCTGTAG TCACTGTTAT GTTAAATCCA GAAGTTACAC 1701 TAGAGCCAGC GATACTTTAT TTGTAGACAA TCAATTTGAA TCCATATGTT 1751 ATTACTGGCA GATGATACAT GATTACAGTT CTGAATCTGT AACACTTACA 1801 AAAGGAAACC CAGAGCAGCT TGATGAGTTT TTGTTTCTGC TTCGTTCCTG 1851 GGAGTCAGTA GAAACAGCAG TTGTATGTGG TTATGTTAGT CTCAAGATAC 1901 TTAATTTGTT GACCTTACTT CAGAAAAATT TTGTATGTAT TATATTTGTG 1951 GGAAGGTAAA ATAATCATTT GAGATTTTTA TCAAATATGA AGATTAGTTA 2001 TTTATGAAAA ACAAAGAAAT GTCTATTTTT CTTTGTTCCC AATTAATGTA 2051 GATAAATTTT AAAATGCATT AAAGTAATGG TCCGGAAAAA AAAAA BLAST Results No BLAST result Medlme entries 89098903: Human interleukin 7: molecular cloning and growth factor activity on human and murine B-lmeage cells. Peptide information for frame 2 ORF from 368 bp to 679 bp; peptide length: 104 Category: similarity to known protein 1 METSHAHESN CKIKGYGVVQ QLLHSQLCGA GEDLGPIGVS YVYSFRAVPF 51 SLILSNAΞLH ΞLGGKDSIQV GCLKELMGPL SELADQILGN LFNFYDFPSH 101 ILKW BLASTP hits Entry B32223 from database PIR: ιnterleukm-7 precursor (clone 1) - human Score = 66, P = 7.0e-01, identities = 21/70, positives = 33/70 Alert BLASTP hits for DKFZphtes3_35e21, frame 2 PIR:B32223 mterleukm-7 precursor (clone 1) - human, N = 1, Score = 66, P = 0.72 TREMBL :PADAL1_1 gene: "dall"; P.abies dall mRNA, N = 2, Score = 59, P = 0.77 PIR:C32223 ιnterleukιn-7 precursor (clone 4) - human, N = 1, Score = 66, P = 0.79 TREMBL :PRU76726_1 gene: "PrMADS3"; product: "MADS-box protein"; Pinus radiata MADS-box protein (PrMADS3) mRNA, complete eds., N = 2, Score = 59, P = 0.94 >PIR:B32223 ιnterleukιn-7 precursor (clone 1) - human Length = 133 HSPs: Score = 66 (9.9 bits), Expect = 1.3e+00, P = 7.2e-01 Identities = 21/68 (30%), Positives = 33/68 (48%) Query: 39 VSYVYSFRAVPFSLIL SNASLHSLGGK—DSIQVGCLKELMGPLSELADQILGNL 91 VS+ Y F P L+L S+ + GK +S+ + + +L+ + E+ L N Sbjct: 4 VSFRYIFGLPPLILVLLPVASSDCDIEGKDGKQYESVLMVSIDQLLDSMKEIGΞNCLNNE 63 Query: 92 FNFYDFPSHI 101 FNF F HI Sbjct: 64 FNF--FKRHI 71 Pedant information for DKFZphtes3_35e21, frame 2 Report for DKFZphtes3_35e21.2 [LENGTH] 104 [MW] 11339.12 [pi] 5.87 [PROSITE] MYRISTYL 2 [PROSITE] PKC PHOSPHO SITE 1 [PROSITE] ASN_GLYCOSYLATION 1 [KW] Alpha_Beta SEQ METSHAHESNCKIKGYGVVQQLLHSQLCGAGEDLGPIGVSYVYSFRAVPFSLILSNASLH PRD ccchhhhhcccccccchhhhhhhhhhhcccccccccceeeeeeeccccceeeeecccccc SEQ SLGGKDSIQVGCLKELMGPLSELADQILGNLFNFYDFPSHILKW PRD cccccceeeccccccccccchhhhhhhhcccccccccccccccc Prosite for DKFZphtes3_35e21.2 PS00001 56- ->60 ASN GLYCOSYLATION PDOC00001 PS00005 44- ->47 PKC PHOSPHO_SITE PDOC00005 PS00008 63- ->69 MYRISTYL PDOC00008 PS00008 89- ->95 MYRISTYL PDOC00008 (No Pfam data available for DKFZphtes3_35e21.2) DKFZphtes3_35g6 group: testes derived DKFZphtes3_35g6 encodes a novel 482 amino acid protein with high partial similarity to H. sapiens chromosome 19, cosmid R27216. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . strong similarity to R27216_l complete cDNA, complete eds, EST hits Sequenced by DKFZ Locus: /map="15" Insert length: 3177 bp Poly A stretch at pos. 3167, polyadenylation signal at pos. 3148 1 GGAGGCAGCG CCGGCCTCCG GAGGCGGCCT GGGCGATGGC GGCGGAGTTT 51 TGTCCATAAC CTGGGCAACC GCGCAGCTGG AGGATGGCCT CACTCGGGCC 101 TGCCGCAGCT GGGGAGCAGG CGTCGGGGGC TGAGGCGGAG CCGGGCCCCG 151 CGGGGCCGCC GCCGCCGCCC TCACCGTCCT CTCTGGGGCC CCTGCTCCCC 201 CTGCAGCGGG AACCTCTCTA CAACTGGCAG GCGACCAAGG CGTCGCTGAA 251 GGAGCGCTTC GCCTTCCTCT TCAACTCGGA GCTGCTGAGC GATGTGCGCT 301 TCGTACTGGG CAAGGGTCGC GGCGCCGCCG CCGCTGGGGG CCCGCAGCGC 351 ATCCCCGCCC ACCGCTTCGT GCTGGCGGCC GGCAGCGCCG TCTTTGACGC 401 CATGTTCAAC GGCGGCATGG CCACCACGTC GGCCGAGATC GAGCTGCCGG 451 ACGTGGAGCC CGCAGCCTTC CTGGCGCTGC TGAGATTTCT ATATTCAGAT 501 GAAGTTCAAA TTGGTCCAGA AACAGTTATG ACCACTCTTT ATACTGCCAA 551 GAAATACGCA GTCCCAGCCT TGGAAGCACA CTGTGTAGAA TTTCTCACCA 601 AACATCTTAG GGCAGATAAT GCCTTTATGT TACTTACTCA GGCTCGATTA 651 TTTGATGAAC CTCAGCTTGC TAGTCTTTGT CTAGATACAA TAGACAAAAG 701 CACAATGGAT GCAATAAGTG CAGAAGGGTT TACTGATATT GATATAGATA 751 CACTCTGTGC AGTTTTAGAG AGAGACACAC TCAGTATTCG AGAAAGTCGA 801 CTTTTTGGAG CTGTTGTACG CTGGGCAGAA GCAGAATGTC AGAGACAACA 851 ATTACCTGTG ACTTTTGGGA ATAAACAAAA AGTTCTAGGA AAAGCACTTT 901 CCTTAATCCG GTTCCCACTG ATGACAATTG AGGAATTTGC AGCAGGTCCT 951 GCTCAATCTG GAATTTTGTC AGATCGTGAA GTGGTAAACC TCTTTCTTCA 1001 TTTTACTGTC AACCCTAAAC CCCGAGTTGA ATACATTGAC CGACCAAGAT 1051 GCTGTCTCAG GGGAAAGGAA TGCTGCATCA ATAGATTCCA GCAAGTAGAA 1101 AGCCGCTGGG GTTACAGTGG GACGAGTGAT CGAATCAGAT TCACAGTTAA 1151 TAGAAGGATC TCTATAGTTG GATTTGGCTT GTATGGATCT ATTCATGGCC 1201 CTACAGATTA TCAAGTGAAT ATACAGATCA TTGAATATGA GAAAAAGCAA 1251 ACCCTGGGAC AGAATGATAC CGGCTTTAGT TGTGATGGGA CAGCTAACAC 1301 ATTCAGGGTC ATGTTCAAGG AACCCATAGA GATCCTGCCC AATGTGTGCT 1351 ACACAGCATG TGCAACACTC AAAGGTCCAG ATTCCCACTA TGGCACAAAA 1401 GGATTGAAGA AAGTAGTGCA TGAGACACCT GCTGCAAGCA AGACTGTTTT 1451 TTTCTTTTTT AGTTCCCCTG GCAATAATAA TGGCACTTCA ATAGAAGATG 1501 GACAAATTCC AGAAATCATA TTTTATACAT AATTTAGCAT TATAATACAT 1551 CTTGGCTAAA TAATACCATA CAATCTAGTG TCAAAAACAT AAATGGCCAC 1601 AAAAAAGTAG TTTGAGTGTT ATGAATATTT AAAATTGTAA GATAAGAAAC 1651 AGTTTCTTAG AGCAGATAGA AAAATGCTTA TTTAAATCTT TGCATGATTT 1701 AAAAACAGAT TTTCCATTTT CTTACAACTT TAAGAGAAAA GAACTGGGTT 1751 TAATGGTTTA AAAAAAAGCA CAGCTTTTTC ACCTTCATCT TGTATAATTT 1801 CATAGATTGG CTGACTTAGG GTCTTTCAAT AGTTTGGGAA TTGAAAGATT 1851 CTTGTTATAT ATAGCTAGTT TGGGTTTGTT TTTGTTTTAA CTATTTTGAA 1901 GGTTAGGTGA GATGGGCAAA TAGGCTTAAC TATTTTGAAG GTTGGATGAA 1951 AAGAGATGGG TCAGTATTCC TACAGAATTC TTATTAACTC AAATAACTAA 2001 ATTTCAGAAA ATTAAGAAGC TGACTTTATA TTTGGTGGTT TGAAGTATCT 2051 TGTTGTTAGC ATTTGTAATA ATGCTAAAAA AGGCCTAATA AAATGCCCAA 2101 GAAAATATTC AGTGCATTTA TAGAGAAGGA TATTTTGTAG TAGTATAGTA 2151 ATGTGTTATG TAGTACAGTT TTAAAGCTAT AAATGGAATT TTGTGTAAAT 2201 TCACAAAAAT GTGATATAAA CAGGATCTAA GACTGGATTC CCTGTCACTA 2251 AACTGCACCA CTATACCTGT CTCTCTGTGT GGGGGACACT GCTGATGATT 2301 CCCAAGATTG AGATGATGAC GGTGATGACG ACTGGGTGAA CAGCCATCAC 2351 TTCAACATTG TGATAATCCT TCACAGCAAG AAACCGAATA AAATACTAAC 2401 ATTTCTAACA ACTGCTCTGA CATTGTAAAG AGATCCAACA GAATCACTCC 2451 TGCTGAAAAA TACGCTTTCT GCCACCTACA CATTTCTATT TAGGAAGTAA 2501 AATTTGCTTC ATGGTCATGA CCCCATTAGT CAGTGTTACA GCTGTGTTGG 2551 GGATAGGAAG TATATCTGGC AGATTGACAT TTATACACTT TTTTATAAAG 2601 CAGATTTTAA AATATAGTAA CATCCATTTT TTTCCCTTGA AAGTGATTCT 2651 CTTATAAAAA ATGAAAGTGG AGTTTAAGGT ATATCAAATC GTTGTGGAAG 2701 GTGATTAAAA ATCAAAATTC TTTTAAATAT CAACTTAATT TTTTCTAAGT 2751 AAGATACAAA AAATTTTCAT CTAAAGTAAT ATTTCACTTT ATATTGTAAA 2801 GAAGGTAGGT ATATTGGTGG CTGAGGTCTC TTGAAATTGC TAAAGGGAAA 2851 TTTTTCTATG GTAATGCTCT TACGGATATA AGCCTCAGTT AAATGGAATT 2901 ATCTATGGGA TGTGTGGTTC TGGTTAACTA AAAATTAACC AGTAAACACT 2951 CTGTAGTAAC CATTACAGAA AATACTTCTG CCTTAAAAAA TATGATATGC 3001 CAGAGATGAG TTAGTGTTTC TTGACGTTGG AGACCTATAA ATGCCTCATC 3051 TGTTGTACTG AACAATTGAA ACTGCATGCA GCCATAAAAG GGACAAGAAA 3101 CAGAACTGTT TACTAACTTT GGGACATCCC CTGGAGTTTT TAAAAATAAA 3151 TAAATATATA TATATATAAA AAAAAAA BLAST Results Entry G37753 from database EMBL: SHGC-63477 Human Homo sapiens STS genomic. Score = 1627, P = 3.0e-66, identities = 327/329 Entry G37752 from database EMBL: SHGC-63476 Human Homo sapiens STS genomic. Score = 1578, P = 6.2e-64, identities = 320/324 Medlme entries No Medline entry Peptide information for frame 3 ORF from 84 bp to 1529 bp; peptide length: 482 Category: similarity to unknown protein 1 MASLGPAAAG EQASGAEAEP GPAGPPPPPΞ PSSLGPLLPL QREPLYNWQA 51 TKASLKERFA FLFNSELLSD VRFVLGKGRG AAAAGGPQRI PAHRFVLAAG 101 SAVFDAMFNG GMATTSAEIE LPDVEPAAFL ALLRFLYSDE VQIGPETVMT 151 TLYTAKKYAV PALEAHCVEF LTKHLRADNA FMLLTQARLF DEPQLASLCL 201 DTIDKSTMDA ISAEGFTDID IDTLCAVLER DTLSIRESRL FGAVVRWAEA 251 ECQRQQLPVT FGNKQKVLGK ALSLIRFPLM TIEEFAAGPA QSGILΞDREV 301 VNLFLHFTVN PKPRVEYIDR PRCCLRGKEC CINRFQQVES RWGYSGTSDR 351 IRFTVNRRIS IVGFGLYGSI HGPTDYQVNI QIIEYEKKQT LGQNDTGFSC 401 DGTANTFRVM FKEPIEILPN VCYTACATLK GPDSHYGTKG LKKVVHETPA 451 ASKTVFFFFS SPGNNNGTSI EDGQIPEIIF YT BLASTP hits Entry AC005306_2 from database TREMBL: product: "R27216_l"; Homo sapiens chromosome 19, cosmid R27 2 16, complete sequence. Score = 1 2 98, P = 1.9e-13 2 , identities = 245/297, positives = 268/297 Entry CEF38H4_9 from database TREMBLNEW: gene: "F38H4.7"; Caenorhabditis elegans cosmid F38H4 Score = 1237, P = 5.6e-126, identities = 248/446, positives = 322/446 Entry AC004678_1 from database TREMBL: product: "R34094_l"; Homo sapiens chromosome 19, cosmid R34094, complete sequence. Score = 555, P = 1.0e-53, identities = 112/137, positives = 123/137 Alert BLASTP hits for DKFZphtes3_35g6, frame 3 No Alert BLASTP hits found Pedant information for DKFZphtes3_35g6, frame 3 Report for DKFZphtes3_35g6.3 [LENGTH] 482 [MW] 52771.47 [pi] 5.79 [HOMOL] TREMBL:AC005306_2 product: "R27216_l"; Homo sapiens chromosome 19, cosmid R27216, complete sequence, le-142 [BLOCKS] BL01075D Acetate and butyrate kinases family proteins [SUPFAM] POZ domain homology 3e-08 [SUPFAM] A55R protein middle region homology 5e-06 [SUPFAM] A55R protein 5e-06 [SUPFAM] A55R protein carboxyl-terminal homology 5e-06 [PROSITE] MYRISTYL [PROSITE] CAMP_PHOSPHO_SITE [PROSITE] CK2_PHOSPHO_SITE [PROSITE] TYR_PHOSPHO_SITE [PROSITE] PKC_PHOSPHO_SITE [PROSITE] ASN_GLYCOSYLATION [KW] Alpha_Beta [KW] LOW COMPLEXITY 11.20 % SEQ MASLGPAAAGEQASGAEAEPGPAGPPPPPSPΞSLGPLLPLQREPLYNWQATKASLKERFA SEG ....xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD cccccccchhhhhhhhcccccccccccccccccccccccccccccchhhhhhhhhhhhhh SEQ FLFNSELLSDVRFVLGKGRGAAAAGGPQRIPAHRFVLAAGSAVFDAMFNGGMATTSAEIE SEG xxxxxxxxxxx PRD hhhccccccceeeeecccccccccccccchhhhheeecccchhhhhhhhcchhhhhhhee SEQ LPDVEPAAFLALLRFLYSDEVQIGPETVMTTLYTAKKYAVPALEAHCVEFLTKHLRADNA SEG PRD ecccchhhhhhhhhhhhccceeechhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccch SEQ FMLLTQARLFDEPQLASLCLDTIDKSTMDAIΞAEGFTDIDIDTLCAVLERDTLSIRESRL SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccchhhhhhhhhhccccchhhhhh SEQ FGAVVRWAEAECQRQQLPVTFGNKQKVLGKALSLIRFPLMTIEEFAAGPAQSGILSDREV SEG PRD hhhhhhhhhhhhhhhhhccccchhhhhhhhhhhhhhcceeecccccccccccccchhhhh SEQ VNLFLHFTVNPKPRVEYIDRPRCCLRGKECCINRFQQVESRWGYSGTSDRIRFTVNRRIS SEG PRD hhhhheeeccccceeeeecccceeeccceeehhhhhhhhhccccccccccchhhhhceee SEQ IVGFGLYGSIHGPTDYQVNIQIIEYEKKQTLGQNDTGFSCDGTANTFRVMFKEPIEILPN SEG PRD eeeccccccccccchhhhhhhcchhhhhhhhccccccccccccccceeeeeccceeeccc SEQ VCYTACATLKGPDSHYGTKGLKKVVHETPAASKTVFFFFSSPGNNNGTSIEDGQIPEIIF SEG xxxxxx PRD ccceeeeecccccccccccceeeeeeeccccceeeeeeeecccccccccccccccceeec SEQ YT SEG PRD Prosite for DKFZphtes3_35g6.3 PS00001 394->398 ASN_GLYCOSYLATION PDOC00001 PS00001 466->470 ASN_GLYCOSYLATION PDOC00001 PS00004 357->361 CAMP_PHOSPHO_SITE PDOC00004 PS00004 387->391 CAMP_PHOSPHO_SITE PDOC00004 PS00005 54->57 PKC_PHOSPHO_SITE PDOC00005 PS00005 154-M57 PKC_PHOΞPHO_SITE PDOC00005 PS00005 234->237 PKC_PHOSPHO_SITE PDOC00005 PS00005 296->299 PKC_PHOSPHO_SITE PDOC00005 PS00005 348->351 PKC_PHOSPHO_SITE PDOC00005 PS00005 406->409 PKC_PHOSPHO_SITE PDOC00005 PS00005 428->431 PKC_PHOSPHO_SITE PDOC00005 PS00006 14->18 CK2_PHOSPHO_SITE PDOC00006 PS00006 54->58 CK2_PHOSPHO_SITE PDOC00006 PS00006 115->119 CK2_PHOSPHO_SITE PDOC00006 PS00006 206->210 CK2_PHOSPHO_SITE PDOC00006 PS00006 217->221 CK2_PHOSPHO_SITE PDOC00006 PS00006 234->238 CK2_PHOSPHO_SITE PDOC00006 PS00006 281->285 CK2_PHOSPHO_SITE PDOC00006 PS00006 296->300 CK2_PHOSPHO_SITE PDOC00006 PS00006 468->472 CK2_PHOSPHO_SITE PDOC00006 PS00007 430->437 TYR_PHOSPHO_SITE PDOC00007 PS00008 80->86 MYRISTYL PDOC00008 PS00008 110->116 MYRISTYL PDOC00008 PS00008 365->371 MYRISTYL PDOC00008 PS00008 392->398 MYRISTYL PDOC00008 PS00008 402->408 MYRISTYL PDOC00008 PS00008 463->469 MYRISTYL PDOC00008 (No Pfam data available for DKFZphtes3_35g6.3) DKFZphtes3_35kl6 group: metabolism DKFZphtes3_35kl6 encodes a novel 666 ammo acid protein with weak similarity to fatty acid-CoA synthetaseses/ligases . The novel protein contains a putative AMP-binding domain signature, which is present in enzymes, which act via an ATP-dependent covalent binding of AMP to their substrate. This domain is found in several CoA synthetases, such as acetate-CoA ligase (EC 6.2.1.1), long- chain-fatty-acid-CoA ligase (EC 6.2.1.3), bile acid-CoA ligase. Therefore it is a new fatty acid-CoA synthetasese/ligase with unknown substrate. The new protein can find application in modulation of fatty acid metabolism and as a new enzyme for biotechnologic production processes. similarity to acyl-CoA synthetase complete cDNA, complete eds, potential start codon at Bp 50, few EST hits, seems to be a testis specific cDNA, 5 of 6 EST hits are from testis deπeved librarys Sequenced by DKFZ Locus : unknown Insert length: 2520 bp Poly A stretch at pos. 2510, polyadenylation signal at pos. 2490 1 CAGATGTCCC AGCTCCAGTG CTGTGGAGCA TGGTTTCTGC ACACCTGGAA 51 TGACTGGAAC CCCAAAGACT CAAGAAGGAG CTAAAGATCT TGAAGTAGAC 101 ATGAATAAAA CAGAAGTTAC TCCCAGGCTG TGGACCACCT GTCGAGATGG 151 AGAAGTCCTT CTGAGGCTAT CCAAACACGG ACCAGGCCAT GAGACCCCGA 201 TGACCATCCC TGAATTTTTT CGAGAGTCAG TCAACCGATT TGGAACTTAT 251 CCAGCCCTCG CATCCAAGAA TGGCAAAAAG TGGGAAATTC TGAATTTCAA 301 CCAGTACTAT GAGGCTTGTC GGAAGGCTGC AAAATCCTTG ATCAAGCTGG 351 GTTTGGAGCG TTTCCACGGA GTTGGTATCC TGGGGTTTAA CTCTGCAGAG 401 TGGTTTATCA CTGCTGTTGG TGCCATCCTA GCCGGGGGTC TTTGTGTTGG 451 TATTTATGCC ACCAACTCTG CCGAGGCTTG TCAATATGTC ATCACTCATG 501 CCAAAGTGAA CATCTTGCTG GTTGAGAATG ATCAACAGTT ACAGAAAATC 551 CTTTCGATTC CACAGAGCAG CCTAGAGCCC CTAAAAGCGA TCATCCAGTA 601 CAGACTGCCA ATGAAGAAGA ACAACAACTT GTACTCTTGG GATGATTTCA 651 TGGAACTTGG CAGAAGTATC CCTGACACCC AACTGGAGCA GGTCATCGAG 701 AGCCAGAAGG CGAATCAATG CGCAGTGCTC ATCTACACTT CAGGGACCAC 751 AGGCATACCC AAGGGAGTGA TGCTCAGTCA TGACAACATC ACGTGGATTG 801 CAGGAGCAGT GACAAAGGAC TTTAAACTGA CAGACAAGCA TGAGACGGTG 851 GTTAGCTACC TCCCACTCAG CCATATTGCA GCACAGATGA TGGACATCTG 901 GGTACCCATA AAGATTGGGG CGCTCACATA CTTTGCTCAA GCAGATGCTC 951 TCAAGGGCAC CTTGGTAAGT ACTCTAAAGG AGGTAAAACC TACTGTCTTC 1001 ATTGGAGTGC CTCAAATTTG GGAGAAGATA CATGAGATGG TGAAGAAAAA 1051 TAGTGCCAAG TCCATGGGCT TGAAGAAGAA GGCATTCGTG TGGGCAAGAA 1101 ACATTGGCTT CAAGGTCAAC TCAAAAAAGA TGTTGGGGAA ATATAATACT 1151 CCCGTGAGCT ACCGCATGGC TAAGACTCTC GTGTTCAGCA AAGTCAAGAC 1201 ATCCCTTGGC TTGGATCACT GTCACTCTTT TATCAGTGGG ACTGCGCCCC 1251 TCAACCAAGA GACTGCCGAG TTCTTTCTAA GCTTGGACAT ACCTATAGGC 1301 GAGTTGTATG GGTTGAGTGA GAGCTCGGGA CCCCACACGA TATCCAACCA 1351 GAATAACTAC AGGCTTCTAA GCTGTGGCAA GATCTTGACT GGGTGTAAGA 1401 ATATGCTGTT CCAGCAGAAC AAGGATGGCA TTGGGGAGAT CTGCCTCTGG 1451 GGTAGGCACA TCTTCATGGG CTATCTGGAA AGTGAGACTG AAACTACAGA 1501 GGCCATCGAT GATGAAGGCT GGCTACACTC TGGGGATCTG GGCCAGCTGG 1551 ACGGTCTGGG TTTCCTCTAT GTCACCGGCC ACATCAAAGA AATCCTTATC 1601 ACTGCTGGTG GTGAAAATGT GCCCCCCATT CCTGTTGAGA CCTTGGTTAA 1651 GAAGAAGATC CCCATCATCA GTAACGCCAT GTTAGTAGGA GATAAACTGA 1701 AGTTTCTGAG CATGTTGCTG ACGCTGAAGT GTGAGATGAA TCAGATGAGC 1751 GGAGAACCTC TGGACAAGCT GAACTTCGAG GCCATCAACT TCTGTCGGGG 1801 TCTGGGCAGC CAGGCATCCA CCGTGACTGA GATGGTGAAG CAGCAAGACC 1851 CCCTGGTCTA CAAGGCCATC CAGCAAGGCA TCAATGCTGT GAACCAGGAA 1901 GCCATGAACA ATGCACAGAG GATTGAAAAG TGGGTCATCT TGGAGAAGGA 1951 CTTTTCCATC TATGGTGGAG AGCTAGGTCC AATGATGAAA CTTAAGAGAC 2001 ATTTTGTAGC CCAGAAATAC AAAAAACAAA TTGATCACAT GTACCACTGA 2051 CTGCTTTGAT GGAGCTGCTC TCAGCTGTTC TGATGCCTTC AGCAGGAAGA 2101 CCTCATTGCA ATAAGTGAAA TGCTGCTCTA GGTAGAAGCT CTCCCTGCTG 2151 TTTTTAAGAA GCCACATTCC TCATTGGTCA GTTTCTTGAT TGTTCGTCTG 2201 TTGGAGAGGT GCTCCCTAGA AGAACCTGCC ATACGTTTCA AAGCAATAAA 2251 ATCACTGTAT ATCTTTCTAA GGACCTTCAA GTCATGACTC CAGGGAAGCC 2301 TATTGGGAAG TCTACTAAAA ACTGCCTGAT TTACAAGAAA GACCTGAACT 2351 TGTGGGCTCC CATTTGATTT TTTTCTCCTC AGGGGACTCA GACATTAGAA 2401 AGAAAAAGCC TCACAGATTT GAAGAACTGG ACCCCCAAAT CAACTCACCT 2451 GCCTGGAAGC AACTGGGAAA CCCTTCCAAT AAGTCCTGAT AATAAAGCAC 2501 TTCAGGGTCC AAAAAAAAAA BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 2 ORF from 50 bp to 2047 bp; peptide length: 666 Category: similarity to known protein 1 MTGTPKTQEG AKDLEVDMNK TEVTPRLWTT CRDGEVLLRL ΞKHGPGHETP 51 MTIPEFFRES VNRFGTYPAL ASKNGKKWEI LNFNQYYEAC RKAAKSLIKL 101 GLERFHGVGI LGFNSAEWFI TAVGAILAGG LCVGIYATNS AEACQYVITH 151 AKVNILLVEN DQQLQKILSI PQSSLEPLKA IIQYRLPMKK NNNLYSWDDF 201 MELGRSIPDT QLEQVIESQK ANQCAVLIYT SGTTGIPKGV MLSHDNITWI 251 AGAVTKDFKL TDKHETVVSY LPLSHIAAQM MDIWVPIKIG ALTYFAQADA 301 LKGTLVSTLK EVKPTVFIGV PQIWEKIHEM VKKNSAKSMG LKKKAFVWAR 351 NIGFKVNSKK MLGKYNTPVS YRMAKTLVFS KVKTSLGLDH CHSFISGTAP 401 LNQETAEFFL SLDIPIGELY GLSESSGPHT ISNQNNYRLL SCGKILTGCK 451 NMLFQQNKDG IGEICLWGRH IFMGYLESET ETTEAIDDEG WLHSGDLGQL 501 DGLGFLYVTG HIKEILITAG GENVPPIPVE TLVKKKIPII SNAMLVGDKL 551 KFLSMLLTLK CEMNQMSGEP LDKLNFEAIN FCRGLGSQAS TVTEMVKQQD 601 PLVYKAIQQG INAVNQEAMN NAQRIEKWVI LEKDFSIYGG ELGPMMKLKR 651 HFVAQKYKKQ IDHMYH BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_35kl6, frame 2 TREMBL :AB014531_1 gene: "KIAA0631"; product: "KIAA0631 protein"; Homo sapiens mRNA for KIAA0631 protein, partial eds., N = 1, Score = 1641, P = 8.9e-169 PIR:E70937 probable fadD15 - Mycobacterium tuberculosis (strain H37RV) , N = 2, Score = 532, P = 3.6e-62 PIR:H64041 long-chain-fatty-acid—CoA ligase homolog - Haemophilus influenzae (strain Rd KW20) , N = 2, Score = 486, P = 6.5e-59 >TREMBL:AB014531_1 gene: "KIAA0631"; product: "KIAA0631 protein"; Homo sapiens mRNA for KIAA0631 protein, partial eds. Length = 634 HSPs: Score = 1641 (246.2 bits), Expect = 8.9e-169, P = 8.9e-169 Identities = 319/628 (50%), Positives = 440/628 (70%) Query: 38 LRLSKHGPGHETPMTIPEFFRESVNRFGTYPALASKNGKKWEILNFNQYYEACRKAAKSL 97 LR+ P + P T+ F E+++++G AL K KWE ++++QYY R+AAK Sbjct: 2 LRIDPSCP—QLPYTVHRMFYEALDKYGDLIALGFKRQDKWEHISYSQYYLLARRAAKGF 59 Query: 98 IKLGLERFHGVGILGFNSAEWFITAVGAILAGGLCVGIYATNSAEACQYVITHAKVNILL 157 +KLGL++ H V ILGFNS EWF +AVG + AGG+ GIY T+S EACQY+ N+++ Sbjct: 60 LKLGLKQAHSVAILGFNSPEWFFSAVGTVFAGGIVTGIYTTSSPEACQYIAYDCCANVIM 119 Query: 158 VENDQQLQKILSIPQSSLEPLKAIIQYRLPM-KKNNNLYSWDDFMELGRSIPDTQLEQVI 216 V+ +QL+KIL I L LKA++ Y+ P K N+Y+ ++FMELG +P+ L+ +1 Sbjct: 120 VDTQKQLEKILKI-WKQLPHLKAVVIYKEPPPNKMANVYTMEEFMELGNEVPEEALDAII 178 Query: 217 ESQKANQCAVLIYTSGTTGIPKGVMLSHDNITWIA—GAVTKDFKLTD-KHETVVSYLPL 273 ++Q+ NQC VL+YTSGTTG PKGVMLS DNITW A G+ D + + + E VVΞYLPL Sb j ct: 179 DTQQPNQCCVLVYTSGTTGNPKGVMLSQDNITWTARYGSQAGDIRPAEVQQEVVVSYLPL 238 Query: 274 SHIAAQMMDIWVPIKIGALTYFAQADALKGTLVSTLKEVKPTVFIGVPQIWEKIHEMVKK 333 SHIAAQ+ D+W 1+ GA FA+ DALKG+LV+TL+EV+PT +GVP++WEKI E +++ Sbjct: 239 SHIAAQIYDLWTGIQWGAQVCFAEPDALKGSLVNTLREVEPTSHMGVPRVWEKIMERIQE 298 Query: 334 NSAKSMGLKKKAFVWARNIGFKVNSKKMLGKYNTPVSYRMAKTLVFSKVKTSLGLDHCHS 393 +A+S +++K +WA ++ + N G P + R+A LV +KV+ +LG C Sbjct: 299 VAAQSGFIRRKMLLWAMSVTLEQNLT-CPGSDLKPFTTRLADYLVLAKVRQALGFAKCQK 357 Query: 394 FISGTAPLNQETAEFFLSLDIPIGELYGLSESSGPHTISNQNNYRLLSCGKILTGCKNML 453 G AP+ ET FFL L+I + YGLSE+SGPH +S+ NYRL S GK++ GC+ L Sbjct: 358 NFYGAAPMMAETQHFFLGLNIRLYAGYGLSETSGPHFMSSPYNYRLYSSGKLVPGCRVKL 417 Query: 454 FQQNKDGIGEICLWGRHIFMGYLESETETTEAIDDEGWLHSGDLGQLDGLGFLYVTGHIK 513 Q+ +GIGEICLWGR IFMGYL E +T EAID+EGWLH+GD G+LD GFLY+TG +K Sbjct: 418 VNQDAEGIGEICLWGRTIFMGYLNMEDKTCEAIDEEGWLHTGDAGRLDADGFLYITGRLK 477 Query: 514 EILITAGGENVPPIPVETLVKKKIPIISNAMLVGDKLKFLSMLLTLKCEMNQMSGEPLDK 573 E++ITAGGENVPP+P+E VK ++PI ISNAML+GD+ KFLSMLLTLKC ++ + + D Sbjct: 478 ELIITAGGENVPPVPIEEAVKMELPIISNAMLIGDQRKFLSMLLTLKCTLDPDTSDQTDN 537 Query: 574 LNFEAINFCRGLGSQASTVTEMVKQQDPLVYKAIQQGINAVNQEAMNNAQRIEKWVILEK 633 L +A+ FC+ +GS+A+TV+E+++++D VY+AI++GI VN A I+KW ILE+ Sbjct: 538 LTEQAVEFCQRVGSRATTVSEIIEKKDEAVYQAIEEGIRRVNMNAAARPYHIQKWAILER 597 Query: 634 DFSIYGGELGPMMKLKRHFVAQKYKKQIDHMY 665 DFSI GGELGP MKLKR V +KYK ID Y Sbjct: 598 DFSISGGELGPTMKLKRLTVLEKYKGIIDSFY 629 Pedant information for DKFZphtes3_35kl6, frame 2 Report for DKFZphtes3_35kl6.2 [LENGTH] 666 [MW] 74344.97 [pi] 8.67 [HOMOL] TREMBL:AB014531_1 gene: "KIAA0631" product: "KIAA0631 protein"; Homo sapiens mRNA for KIAA0631 protein, partial eds. le-176 [FUNCAT] l lipid metabolism [H. influenzae, HI0002] 2e-55 [FUNCAT] 08.10 peroxisomal transport [S. cerevisiae, YER015w] 2e-29 [FUNCAT] 30.19 peroxisomal organization [S. cerevisiae, YER015w] 2e-29 [FUNCAT] 01.06.13 lipid and fatty-acid transport [S. cerevisiae, YER015w] 2e-29 [FUNCAT] 01.06.07 lipid, fatty-acid and sterol utilization [S. cerevisiae, YER015w] 2e-29 [FUNCAT] 01.06.01 lipid, fatty-acid and sterol biosynthesis [S. cerevisiae, YMR246w] 2e-23 [FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylation, farnesylation and processing) [S. cerevisiae, YMR246w] 2e-23 [BLOCKS] BL00455 [SCOP] dllci 5.19.1.1.1 Luciferase [Firefly (Phontinus pyralis) le-49 [EC] 1.13.12.7 Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) 9e-17 [EC] 6.2.1.3 Long-chain-fatty-acid—CoA ligase 4e-34 [EC] 5.1.1.11 Phenylalanine racemase (ATP-hydrolysmg) 6e-08 [EC] 6.2.1.12 4-Coumarate—CoA ligase 8e-18 [PIRKW] duplication 6e-07 [PIRKW] phosphopantetheine 3e-12 [PIRKW] multifunctional enzyme 3e-06 [PIRKW] ligase 6e-08 [PIRKW] acid-thiol ligase 4e-34 [PIRKW] transmembrane protein 5e-22 [PIRKW] monooxygenase 9e-17 [PIRKW] hydrolase 4e-34 [PIRKW] peroxisome 9e-15 [PIRKW] antibiotic biosynthesis 3e-12 [PIRKW] isomerase 6e-08 [PIRKW] flavonoid biosynthesis le-17 [PIRKW] magnesium 9e-15 [PIRKW] ATP 5e-22 [PIRKW] oxidoreductase 9e-17 [PIRKW] liver 2e-31 [SUPFAM] alpha-ammoadipyl-cysteinyl-valine synthetase 3e-07 [SUPFAM] human long-chain-fatty-acid—CoA ligase 4e-34 [SUPFAM] gramicidin S synthetase I 6e-08 [SUPFAM] peptide synthetase ppsE 7e-06 [SUPFAM] gramicidin S synthetase I repeat homology 3e-12 [SUPFAM] peptide synthetase ppsD 2e-07 [SUPFAM] probable acyl-CoA ligase medium chain 2e-09 [SUPFAM] acetate—CoA ligase 8e-10 [SUPFAM] acetate—CoA ligase homology 4e-54 [SUPFAM] surfactin synthetase 3e-12 [SUPFAM] 4-coumarate—CoA ligase 8e-18 [SUPFAM] short-chain alcohol dehydrogenase homology 8e-07 [SUPFAM] acyl carrier protein homology 2e-29 [PROSITE] MYRISTYL 12 [PROSITE] AMP_BINDING 1 [PROSITE] AMIDATION 1 [PROSITE] CAMP_PHOSPHO_SITE 1 [PROSITE) CK2_PHOSPHO_SITE 9 [PROSITE] TYR_PHOSPHO_SITE 3 [PROSITE] PKC_PHOSPHO_SITE 10 [PROSITE] ASN_GLYCOSYLATION 2 [PFAM] AMP-binding enzymes [KW] Irregular [KW] 3D [KW] LOW COMPLEXITY 1.80 % SEQ MTGTPKTQEGAKDLEVDMNKTEVTPRLWTTCRDGEVLLRLSKHGPGHETPMTIPEFFREΞ SEG llci- SEQ VNRFGTYPALASKNGKKWEILNFNQYYEACRKAAKΞLIKLGLERFHGVGILGFNSAEWFI SEG SEQ TAVGAILAGGLCVGIYATNSAEACQYVITHAKVNILLVENDQQLQKILSIPQSSLEPLKA SEG SEQ IIQYRLPMKKNNNLYSWDDFMELGRSIPDTQLEQVIESQKANQCAVLIYTSGTTGIPKGV SEG SEQ MLSHDNITWIAGAVTKDFKLTDKHETVVSYLPLSHIAAQMMDIWVPIKIGALTYFAQADA SEG SEQ LKGTLVSTLKEVKPTVFIGVPQIWEKIHEMVKKNSAKSMGLKKKAFVWARNIGFKVNSKK SEG llci- SEQ MLGKYNTPVSYRMAKTLVFSKVKTSLGLDHCHSFISGTAPLNQETAEFFLSLDIPIGELY SEG llci- TTTTCEEETTTTCCCHHHHHHHHHHCCCCBCEE SEQ GLSESSGPHTISNQNNYRLLΞCGKILTGCKNMLFQQNKDGIGEICLWGRHIFMGYLESET SEG llci- ECGGGTTEEEECCCCCCEEEEETTTTEEEEETTTTTCEETTEEEEEETTTTCCEETTTHH SEQ ETTEAIDDEGWLHSGDLGQLDGLGFLYVTGHIKEILITAGGENVPPIPVETLVKKKIPII SEG xxxxxxxxxxxx llci- HHHHHBTTTTCEEEEEEEEETTTTCEEE ECEEETTEEECHHHHHHHHHHT-TTE ΞEQ SNAMLVGDKLKFLSMLLTLKCEMNQMSGEPLDKLNFEAINFCRGLGSQASTVTEMVKQQD SEG llci- EEEEEEE SEQ PLVYKAIQQGINAVNQEAMNNAQRIEKWVILEKDFSIYGGELGPMMKLKRHFVAQKYKKQ SEG SEQ IDHMYH SEG Prosite for DKFZphtes3_35kl6.2 PS00001 19->23 ASN_GLYCOSYLATION PDOC00001 PS00001 246->250 ASN_GLYCOSYLATION PDOC00001 PS00004 332->336 CAMP_PHOSPHO_SITE PDOC00004 PS00005 4->7 PKC_PHOSPHO_SITE PDOC00005 PS00005 24->27 PKC_PHOSPHO_SITE PDOC00005 PS00005 30->33 PKC_PHOSPHO_SITE PDOC00005 PS00005 218->221 PKC_PHOΞPHO_SITE PDOC00005 PS00005 261->264 PKC PHOSPHO SITE PDOC00005 PS00005 308->311 PKC PHOSPHO SITE PDOC00005 PS00005 335->338 PKC PHOSPHO- " SITE PDOC00005 PS00005 358->361 PKC PHOSPHO " " SITE PDOC00005 PS00005 370->373 PKC PHOSPHO " " SITE PDOC00005 PS00005 558->561 PKC PHOSPHO " " SITE PDOC00005 PS00006 30->34 CK2 PHOSPHO " " SITE PDOC00006 PS00006 52->56 CK2 PHOSPHO " SITE PDOC00006 PS00006 173->177 CK2 PHOSPHO " SITE PDOC00006 PS00006 196->200 CK2 PHOSPHO " " SITE PDOC00006 PΞ00006 206->210 CK2 PHOSPHO " SITE PDOC00006 PS00006 210->214 CK2 PHOSPHO " SITE PDOC00006 PS00006 308->312 CK2 PHOSPHO " SITE PDOC00006 PS00006 478->482 CK2 PHOSPHO " SITE PDOC00006 PΞ00006 591->595 CK2 PHOSPHO " SITE PDOC00006 PS00007 659->666 TYR PHOSPHO " SITE PDOC00007 PS00007 658->666 TYR PHOSPHO " SITE PDOC00007 PS00007 597->605 TYR PHOSPHO " SITE PDOC00007 PS00008 3->9 MYRISTYL PDOC00008 PS00008 65->71 MYRISTYL PDOC00008 PΞ00008 124->130 MYRISTYL PDOC00008 PS00008 130->136 MYRISTYL PDOC00008 PS00008 134->140 MYRISTYL PDOC00008 PS00008 235->241 MYRISTYL PDOC00008 PS00008 239->245 MYRISTYL PDOC00008 PS00008 303->309 MYRISTYL PDOC00008 PS00008 387->393 MYRISTYL PDOC00008 PS00008 421->427 MYRISTYL PDOC00008 PS00008 498->504 MYRISTYL PDOC00008 PS00008 586->592 MYRISTYL PDOC00008 PS00009 74->78 AMIDATION PDOC00009 PS00455 227->239 AMP BINDING PDOC00427 Pfam for DKFZphtes3_35kl6.2 HMM_NAME AMP-bindmg enzymes HMM *TYRELNERANRLARHLRsekGIrPGDιVgIMMDRSMWMIVaMLGIWKAG + + +E +A L+ +G VGI+ +S + ++ G + AG Query 82 NFNQYYEACRKAAKSLI-KLGLERFHGVGILGFNSAEWFITAVGAILAG 129 HMM GAYVPIDPeYPdERIqYMLEDSGArLLITQrh.... HmqRIPdemwwvdH G +V I +E QY++ ++ + +L+++ + + IP++++ + Query 130 GLCVGIYATNSAEACQYVITHAKVNILLVENDQQLQKILSIPQSSLEPLK 179 HMM IiviDWe WddlWWHedeeNpqpWvdPeDLAYIIY +I++ + + ++++ + E ++ ++++ A +IY Query 180 AIIQYRLPMKKNNNLYSWDDFMELGRSIPDTQLEQVIESQKANQCAVLIY 229 HMM TSGTTGKPKGVMIEHrNIvNycqWMnWRYgMteeDDRILWFtSDpYWFDa TSGTTG PKGVM++H NI+ + +++ +T+ +++ + + ++ A Query 230 TSGTTGIPKGVMLSHDNITWIAGAVTKDFKLTDKHETVVSYLP-LSHIAA 278 HMM SVWDMFWpLLnGaTLYIpPeEtRrDPerWWqYIqRHglTWWylTPSMFRM +++D++ P+ GA Y + ++ + ++++ ++T+ ++P +++ Query 279 QMMDIWVPIKIGALTYFAQADAL—KGTLVSTLKEVKPTVFIGVPQIWEK 326 HMM LMpd + + Query 327 IHEMVKKNSAKSMGLKKKAFVWARNIGFKVNSKKMLGKYNTPVSYRMAKT 376 HMM psLRhVMFgGEpLsPehWdWWRkrfgfkgRIINMYWPT ++ + +++G PL++E+++ ++ + ++I Y+ + Query 377 LVFSKVKTSLGLDHCHSFISGTAPLNQETAEFFL-ΞLD—IPIGELYGLS 423 HMM ETTVWtTwMrliPdepeqWrwiPIGRPIpNTqWYIMDdnMQIQPiGViGE E++ T+ + + R +++G+ + + + + +N G IGE Query 424 ESSGPHTISNQNN—Y RLLSCGKILTGCKNMLFQQN KDG-IGE 463 HMM LYIgGWPGVARGYWNRPELTEERFipNPFWPGEYRrGWNrRMYRTGDLAR +++ G ++ GY+ + +T E+ + ++ ++GDL++ Query 464 ICLWG-RHIFMGYLESETETTEAIDDEGW LHSGDLGQ 499 HMM WlPDGnlEYLGRID.DQVKIRGYRIELGEIEhqLr.qHPglqEAVV* + G+++ G I + G+++ + +E+ + ++P 1+ A Query 500 LDGLGFLYVTGHIKEILITAGGENVPPIPVETLVKKKIPIISNAML 545 DKFZphtes3_35k24 group: transmembrane protein DKFZphtes3_35k24 encodes a novel 514 amino ac d protein without similarity to known proteins. The novel protein contains 5 transmembrane regions. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes and as a new marker for testicular cells. unknown ; membrane regions : 5 Summary DKFZphtes3_35k24 encodes a novel 514 amino acid protein. No homolouges found in bacteria yeast and C. elegans, specific for mammalians? unknown complete cDNA, complete eds, few EST hits Sequenced by DKFZ Locus : unknown Insert length: 2706 bp Poly A stretch at pos. 2696, polyadenylation signal at pos. 2675 1 CCGTGTGCAG TCGCCCCGCG CCCCGCGCGA CCCTTCGGGT AAACTACGAA 51 CTGGGAGTTC TGAAGAATGG GTAAAGACTT TCGTTACTAT TTCCAGCATC 101 CCTGGTCTCG CATGATTGTG GCTTACTTGG TGATCTTCTT TAACTTCTTA 151 ATATTTGCGG AGGACCCAGT TTCTCATAGC CAAACAGAAG CCAATGTTAT 201 TGTTGTTGGA AACTGTTTTT CATTTGTTAC AAATAAATAC CCTAGAGGAG 251 TTGGCTGGAG GATTTTGAAG GTGCTTCTAT GGCTACTTGC CATTCTCACA 301 GGACTAATAG CTGGCAAATT TCTGTTCCAT CAGCGTTTGT TTGGTCAGTT 351 GCTCCGATTA AAAATGTTTC GAGAAGATCA TGGGTCGTGG ATGACAATGT 401 TCTTCAGCAC AATTCTCTTT CTCTTCATAT TTTCTCACAT ATACAACACG 451 ATTCTTCTAA TGGATGGGAA CATGGGAGCA TATATCATTA CAGACTATAT 501 GGGCATCCGA AATGAAAGTT TCATGAAATT AGCTGCAGTA GGGACCTGGA 551 TGGGGGACTT TGTCACAGCT TGGATGGTCA CTGATATGAT GCTTCAGGAC 601 AAACCCTATC CTGACTGGGG AAAATCAGCA AGAGCTTTCT GGAAGAAAGG 651 AAATGTTAGG ATCACTTTAT TCTGGACAGT TCTTTTTACT CTGACGTCTG 701 TGGTTGTACT TGTGATTACA ACGGACTGGA TCAGCTGGGA CAAGCTGAAT 751 CGGGGATTTT TGCCCAGTGA TGAAGTTTCC AGAGCATTCC TTGCTTCTTT 801 TATCTTGGTC TTTGACCTTC TTATTGTGAT GCAGGACTGG GAATTCCCAC 851 ATTTCATGGG AGATGTTGAT GTAAATCTCC CTGGTTTGCA CACCCCTCAC 901 ATGCAGTTCA AGATTCCTTT CTTCCAGAAA ATCTTCAAGG AGGAATATCG 951 TATTCACATA ACAGGCAAAT GGTTTAACTA TGGAATTATC TTCCTCGTCT 1001 TGATTTTGGA TCTTAATATG TGGAAGAACC AAATATTTTA TAAACCTCAT 1051 GAATATGGGC AATATATCGG CCCGGGGCAG AAGATATATA CAGTGAAAGA 1101 CTCAGAAAGT TTAAAAGATT TGAACAGAAC CAAGCTATCC TGGGAATGGA 1151 GGTCCAATCA CACTAACCCT CGGACTAATA AAACATATGT TGAGGGAGAC 1201 ATGTTCTTAC ACAGCAGGTT CATAGGAGCC AGTCTTGATG TCAAGTGTCT 1251 GGCCTTTGTT CCAAGCCTGA TAGCCTTTGT GTGGTTTGGA TTCTTTATTT 1301 GGTTCTTTGG ACGATTTTTG AAAAATGAGC CACGCATGGA GAATCAAGAC 1351 AAAACTTACA CTCGCATGAA AAGAAAATCT CCATCAGAAC ATAGCAAAGA 1401 CATGGGAATC ACTCGAGAAA ACACCCAGGC TTCAGTAGAA GACCCCTTGA 1451 ATGACCCTTC TTTGGTTTGC ATCAGGTCTG ACTTCAATGA GATCGTCTAC 1501 AAGTCTTCCC ACCTAACCTC GGAAAACTTG AGCTCACAGT TGAACGAATC 1551 TACTAGTGCA ACAGAAGCTG ATCAAGACCC AACGACTTCT AAAAGTACAC 1601 CTACGAACTA GACTCGGAGA TAGACTTGGA GATAACACAA AAAGCAACCT 1651 TGAGTGTAAC TTTAAAAATT TAGTCTTTCC TTTTGTATAT GTAAGGTTTA 1701 CGTAGTGTTA GGTAAAAATA TGAACAATGC CACAACGGTG CTCAACATGC 1751 TTTTTCTAGG ATTCATTGTT TTCTATTTGT ATTATAATAC ACGTGCCTAC 1801 TGTATACTCA ACAGTCCTCT AGAGATTGCT TTTCACAATT GCACAAGCTA 1851 TTACTGACTT TACAGCATAG TGGAAGATTA GCTGATGACC CATGTATCTG 1901 ATGTTCAACC ATAGTGGTGC CTTGAGACAT TAAACTGTTT TTAACTGTAC 1951 CAGAAATGAA GTGTGGAACA GTTACCTAAC CTATTTCACA TGGGCGTTTT 2001 GTATACAACT ATTTTGATCT ACACTTGATG TCTGAGCAGA AAACAGAAAT 2051 AGCTAAATGT GACTCAGGAA GTATCTCTTG GTTTCTTATT CAGCAGCAGA 2101 GTTGGTGACT TTGACAACTG GACTGCAGAG AAACATGGTG ATCACCTTTT 2151 AATTTTTATT GGCTGTCTGC CAAATATAAA TACAGATGCA AAATTCAGTA 2201 ATAGGAGATC CATAACCCAA CATGGGTCAC TACTCGTGAA ATGTGACTTT 2251 CTCCCACCAG TAATTGAAAT GAGGTGATGA TACCTAATTA TGTTTTCCTA 2301 ATTAAAGATA AATTGCTACT TGATTAAAAA TCCTGCCCTT CACCTTTGGG 2351 AACAAAGGTT AAGAGACACA GTTGGGCGAA CTCTCAAATT TATTGGCATT 2401 TACACAAAGT CCCAGACAAC CAAGGAACTG AAGTTTTCAT CATATGAGAG 2451 CAGCACATCC CACCATTTAC AATATTCGTA TATCTTTCTG CAAATATGGC 2501 TCTGGATAGT GAAAATTGAA AAACATATGC CAACCCTGAG CAAGGGAACT 2551 CCTCAAAAAA TCATGCAGCG GAACCTTGTC AGGTAGAGAA GCCGTGCATG 2601 AAAGAATTTG TTTAATGTCT TGTTTTGCGT ATGTGTTTTT TGTTTTTGTT 2651 TTTTAAGAAC TAAATATTGC ACATTAATAA ATAAGAATTA TACAGCAAAA 2701 AAAAAA BLAST Results No BLAST result Medlme entries No Medline entry Peptide information for frame 1 ORF from 67 bp to 1608 bp; peptide length: 514 Category: putative protein 1 MGKDFRYYFQ HPWSRMIVAY LVIFFNFLIF AEDPVSHSQT EANVIWGNC 51 FSFVTNKYPR GVGWRILKVL LWLLAILTGL IAGKFLFHQR LFGQLLRLKM 101 FREDHGSWMT MFFSTILFLF IFSHIYNTIL LMDGNMGAYI ITDYMGIRNE 151 SFMKLAAVGT WMGDFVTAWM VTDMMLQDKP YPDWGKSARA FWKKGNVRIT 201 LFWTVLFTLT ΞVVVLVITTD WISWDKLNRG FLPSDEVSRA FLASFILVFD 251 LLIVMQDWEF PHFMGDVDVN LPGLHTPHMQ FKIPFFQKIF KEEYRIHITG 301 KWFNYGIIFL VLILDLNMWK NQIFYKPHEY GQYIGPGQKI YTVKDSESLK 351 DLNRTKLSWE WRSNHTNPRT NKTYVEGDMF LHSRFIGASL DVKCLAFVPS 401 LIAFVWFGFF IWFFGRFLKN EPRMENQDKT YTRMKRKSPS EHSKDMGITR 451 ENTQASVEDP LNDPSLVCIR SDFNEIVYKS SHLTSENLSS QLNESTSATE 501 ADQDPTTSKS TPTN BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_35k24, frame 1 No Alert BLASTP hits found Pedant information for DKFZphtes3_35k24, frame 1 Report for DKFZphtes3_35k24.1 [LENGTH] 514 [MW] 60185.03 [pi] 8.67 [PROSITE] MYRISTYL 5 [PROSITE] CAMP_PHOSPHO_SITE 1 [PROSITE] CK2_PHOSPHO_SITE 8 [PROSITE] TYR_PHOSPHO_SITE 1 [PROSITE] PKC_PHOSPHO_SITE 7 [PROSITE] ASN_GLYCOSYLATION 6 [KW] ΞIGNAL_PEPTIDE 32 [KW] TRANSMEMBRANE 5 [KW] LOW COMPLEXITY 15.37 % SEQ MGKDFRYYFQHPWSRMIVAYLVIFFNFLIFAEDPVSHΞQTEANVIVVGNCFΞFVTNKYPR SEG PRD cccceeeeeecccchhhhhhhhhhhhhhhhccccccccccceeeeeecccceeeeccccc MEM SEQ GVGWRILKVLLWLLAILTGLIAGKFLFHQRLFGQLLRLKMFREDHGSWMTMFFSTILFLF SEG xxxxxxxxxxxxxxxxx xxxxxxxxxxxx PRD cchhhhhhhhhhhhhhhhhchhhhhhhhhhhhhhhhhhhhhhhccccceeeehhhhhhhh MEM MMMMMMMMMMMMMMMMM MMMMM SEQ IFSHIYNTILLMDGNMGAYIITDYMGIRNESFMKLAAVGTWMGDFVTAWMVTDMMLQDKP SEG xxx PRD hhhhhhhhhhccccccceeeeecccccchhhhhhhhhhccccccccchhhhhhhhhhccc MEM MMMMMMMMMMMM SEQ YPDWGKSARAFWKKGNVRITLFWTVLFTLTSVVVLVITTDWISWDKLNRGFLPSDEVSRA SEG xxxxxxxxxxxxxxxxxxxxx PRD cccccchhhhhhhcccceeehhhhhhhhhhhheeeeecccccccccccccccccchhhhh MEM MMMMMMMMMMMMMMMMM M SEQ FLASFILVFDLLIVMQDWEFPHFMGDVDVNLPGLHTPHMQFKIPFFQKIFKEEYRIHITG SEG xxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhcccccccccccccccccccccccccchhhhhhhhhhhhhhcccc MEM MMMMMMMMMMMMMMMM SEQ KWFNYGIIFLVLILDLNMWKNQIFYKPHEYGQYIGPGQKIYTVKDSESLKDLNRTKLSWE SEG PRD ccceeeeeehhhhhhhcccccceeeccccccccccccceeeeecccccccccccchhhhh MEM SEQ WRSNHTNPRTNKTYVEGDMFLHSRFIGASLDVKCLAFVPSLIAFVWFGFFIWFFGRFLKN SEG xxxxxxxxxxxxxx ... PRD hhcccccccccccccccchhhhhhccccccceeeeeehhhhheeeeccceeeeeeeeccc MEM MMMMMMMMMMMMMMMMM SEQ EPRMENQDKT TRMKRKSPSEHSKDMGITRENTQASVEDPLNDPSLVCIRSDFNEIVYKS SEG PRD cccccccccchhhhhhccccccccccceeeccccccccccccccceeeeccccceeeeec MEM SEQ SHLTSENLSSQLNESTSATEADQDPTTSKSTPTN SEG PRD cccccccccccccccccccccccccccccccccc MEM Prosite for DKFZphtes3_35k24.1 PS00001 149->153 ASN_GLYCOSYLATION PDOC00001 PS00001 353->357 ASN_GLYCOSYLATION PDOC00001 PS00001 364->368 ASN_GLYCOSYLATION PDOC00001 PΞ00001 371->375 ASN_GLYCOSYLATION PDOC00001 PS00001 487->491 ASN_GLYCOSYLATION PDOC00001 PS00001 493->497 ASN_GLYCOSYLATION PDOC00001 PS00004 435->439 CAMP_PHOSPHO_SITE PDOC00004 PS00005 55->58 PKC_PHOSPHO_SITE PDOC00005 PS00005 187->190 PKC_PHOSPHO_SITE PDOC00005 PS00005 299->302 PKC_PHOSPHO_SITE PDOC00005 PS00005 342->345 PKC_PHOSPHO_SITE PDOC00005 PS00005 348->351 PKC_PHOΞPHO_SITE PDOC00005 PS00005 370->373 PKC_PHOSPHO_SITE PDOC00005 PS00005 507->510 PKC_PHOSPHO_SITE PDOC00005 PS00006 38->42 CK2_PHOSPHO_SITE PDOC00006 PS00006 342->346 CK2_PHOSPHO_SITE PDOC00006 PS00006 348->352 CK2_PHOSPHO_SITE PDOC00006 PS00006 373->377 CK2_PHOSPHO_SITE PDOC00006 PS00006 438->442 CK2_PHOSPHO_SITE PDOC00006 PS00006 456->460 CK2_PHOSPHO_SITE PDOC00006 PS00006 497->501 CK2_PHOSPHO_SITE PDOC00006 PS00006 499->503 CK2_PHOSPHO_SITE PDOC00006 PS00007 326->334 TYR_PHOSPHO_SITE PDOC00007 PS00008 48->54 MYRISTYL PDOC00008 PS00008 79->85 MYRISTYL PDOC00008 PS00008 106->112 MYRISTYL PDOC00008 PS00008 134->140 MYRISTYL PDOC00008 PS00008 159->165 MYRISTYL PDOC00008 (No Pfam data available for DKFZphtes3_35k24.1) DKFZphtes3_35nl2 group: metabolism DKFZphtes3_35nl2 encodes a novel 315 amino acid protein with strong similarity to ADP, ATP carrier T (ANT) proteins. The novel protein contains three mitochondrial energy transfer signatures and is closely related to the ADP/ATP translocator, or ademne nucleotide translocator (ANT) , a protein most abundant in mitochondria. In its functional state, it is a homodimer of 30-kD subunits embedded asymmetrically m the inner mitochondrial membrane. The dimer forms a gated pore through which ADP is moved from the matrix into the cytoplasm. The new protein can find application in modulation of ADP-transport and energy metabolism in cells/mitochondria . strong similarity to ADP/ATP carrier proteins EST hits to mouse and drosophila Sequenced by DKFZ Locus : unknown Insert length: 1803 bp Poly A stretch at pos. 1793, polyadenylation signal at pos. 1772 1 AGCGTCCCAA GAGCCACTTT CTCGCCAGTA CGATGCTGCA GCGGTTTTCC 51 GGTTTTCCGC TTCCCTTCAT CGTAGCTCCC GTACTCATTT TTAGCCACTG 101 CTGCCGGTTT TTATATCCTT CTCCATCATG CATCGTGAGC CTGCGAAAAA 151 GAAGGCAGAA AAGCGGCTGT TTGACGCCTC ATCCTTCGGG AAGGACCTTC 201 TGGCCGGCGG AGTCGCGGCA GCTGTGTCCA AGACAGCGGT GGCGCCCATC 251 GAGCGGGTGA AGCTGCTGCT GCAGGTGCAG GCGTCGTCGA AGCAGATCAG 301 CCCCGAGGCG CGGTACAAAG GCATGGTGGA CTGCCTGGTG CGGATTCCTC 351 GCGAGCAGGG TTTCTTCAGT TTTTGGCGTG GCAATTTGGC AAATGTTATT 401 CGGTATTTTC CAACACAAGC TCTAAACTTT GCTTTTAAGG ACAAATACAA 451 GCAGCTATTC ATGTCTGGAG TTAATAAAGA AAAACAGTTC TGGAGGTGGT 501 TTTTGGCAAA CCTGGCTTCT GGTGGAGCTG CTGGGGCAAC ATCCTTATGT 551 GTAGTATATC CTCTAGATTT TGCCCGAACC CGATTAGGTG TCGATATTGG 601 AAAAGGTCCT GAGGAGCGAC AATTCAAGGG TTTAGGTGAC TGTATTATGA 651 AAATAGCAAA ATCAGATGGA ATTGCTGGTT TATACCAAGG GTTTGGTGTT 701 TCAGTACAGG GCATCATTGT GTACCGAGCC TCTTATTTTG GAGCTTATGA 751 CACAGTTAAG GGTTTATTAC CAAAGCCAAA GAAAACTCCA TTTCTTGTCT 801 CCTTTTTCAT TGCTCAAGTT GTGACTACAT GCTCTGGAAT ACTTTCTTAT 851 CCCTTTGACA CAGTTAGAAG ACGTATGATG ATGCAGAGTG GTGAGGCTAA 901 ACGGCAATAT AAAGGAACCT TAGACTGCTT TGTGAAGATA TACCAACATG 951 AAGGAATCAG TTCCTTTTTT CGTGGCGCCT TCTCCAATGT TCTTCGCGGT 1001 ACAGGGGGTG CTTTGGTGTT GGTATTATAT GATAAAATTA AAGAATTCTT 1051 TCATATTGAT ATTGGTGGTA GGTAATCGGG AGAGTAAATT AAGAAATAAC 1101 ATGGATTTAA CTTGTTAAAC ATACAAATTA CATAGCTGCC ATTTGCATAC 1151 ATTTTGATAG TGTTATTGTC TGTATTTTGT TAAAGTGCTA GTTCTGCAAT 1201 AAAGCATACA TTTTTTCAAG AATTTAAATA CTAAAAATCA GATAAATGTG 1251 GATTTTCCTC CCACTTAGAC TCAAACACAT TTTAGTGTGA TATTTCATTT 1301 ATTATAGGTA GTATATTTTA ATTTGTTAGT TTAAAATTCT TTTTATGATT 1351 AAAAATTAAT CATATAATCC TAGATTAATG CTGAAATCTA GGAAATGAAA 1401 GTAGCGTCTT TTAAATTGCT ATTCATTTAA TATACCTGTT TTCCCATCTT 1451 TTGAAGTCAT ATGGTATGAC ATATTTCTTA AAAGCTTATC AATAGATGTC 1501 ATCATATGTG TAGGCAGAAA TAAGCTTTGT TCTATATCTC TTCTAAGACA 1551 GTTGTTATTA CTGTGTATAA TATTTACAGT ATCAGCCTTT GATTATAGAT 1601 GTGATCATTT AAAATTTGAT AATGACTTTA GTGACATTAT AAAACTGAAA 1651 CTGGAAAATA AAATGGCTTA TCTGCTGATG TTTATCTTTA AAATAAATAA 1701 AATCTTGCTA GTGTGAATAT ATCTTAGAAC AAAAGGTATC CTCTTGAAAA 1751 TTAGTTTGTA TATTTTGTTG ACAATAAAGG AAGCTTAACT GTTAAAAAAA 1801 AAA BLAST Results No BLAST result Medline entries 96289608: Molecular biological and quantitative abnormalities of ADP/ATP carrier protein in cardiomyopathic hamsters. Peptide information for frame 2 ORF from 128 bp to 1072 bp; peptide length: 315 Category: strong similarity to known protein Classification: Metabolism Prosite motifs: MITOCH_CARRIER (40-50) MITOCH_CARRIER (145-155) MITOCH CARRIER (242-252) 1 MHREPAKKKA EKRLFDASSF GKDLLAGGVA AAVSKTAVAP IERVKLLLQV 51 QASSKQISPE ARYKGMVDCL VRIPREQGFF SFWRGNLANV IRYFPTQALN 101 FAFKDKYKQL FMSGVNKEKQ FWRWFLANLA SGGAAGATSL CVVYPLDFAR 151 TRLGVDIGKG PEERQFKGLG DCIMKIAKSD GIAGLYQGFG VSVQGIIVYR 201 ASYFGAYDTV KGLLPKPKKT PFLVSFFIAQ VVTTCSGILS YPFDTVRRRM 251 MMQSGEAKRQ YKGTLDCFVK IYQHEGISSF FRGAFSNVLR GTGGALVLVL 301 YDKIKEFFHI DIGGR BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_35nl2, frame 2 PIR:S37210 ADP, ATP carrier protein TI - mouse, N = 1, Score = 1127, P = 2.7e-114 PIR:A44778 ADP, ATP carrier protein TI - human, N = 1, Score = 1125, P = 4.4e-114 TREMBL :DMADPATPT_2 product: "ADP/ATP translocase"; Drosophila melanogaster gene encoding ADP/ATP translocase, N = 1, Score = 1124, P = 5.6e-114 PIR:XWBO ADP, ATP carrier protein TI - bovine, N = 1, Score = 1121, P = 1.2e-113 >PIR:S37210 ADP, ATP carrier protein TI - mouse Length = 298 HSPs: Score = 1127 (169.1 bits), Expect = 2.7e-114, P = 2.7e-114 Identities = 214/293 (73%), Positives = 248/293 (84%) Query: 17 ASSFGKDLLAGGVAAAVSKTAVAPIERVKLLLQVQASSKQISPEARYKGMVDCLVRIPRE 76 A SF KD LAGG+AAAVSKTAVAPIERVKLLLQVQ +SKQIS E +YKG++DC+VRIP+E Sbjct: 5 ALSFLKDFLAGGIAAAVSKTAVAPIERVKLLLQVQHASKQISAEKQYKGIIDC VRIPKE 64 Query: 77 QGFFΞFWRGNLANVIRYFPTQALNFAFKDKYKQLFMSGVNKEKQFWRWFLANLASGGAAG 136 QGF SFWRGNLANVIRYFPTQALNFAFKDKYKQ+F+ GV++ KQFWR+F NLASGGAAG Sbjct: 65 QGFLSFWRGNLANVIRYFPTQALNFAFKDKYKQIFLGGVDRHKQFWRYFAGNLASGGAAG 124 Query: 137 ATSLCVVYPLDFARTRLGVDIGKGPEERQFKGLGDCIMKIAKSDGIAGLYQGFGVSVQGI 196 ATSLC VYPLDFARTRL D+GKG +R+F GLGDC+ KI KSDG+ GLYQGF VSVQGI Sbjct: 125 ATSLCFVYPLDFARTRLAADVGKGSSQREFNGLGDCLTKIFKSDGLKGLYQGFSVSVQGI 184 Query: 197 IVYRASYFGAYDTVKGLLPKPKKTPFLVSFFIAQVVTTCSGILSYPFDTVRRRMMMQSGE 256 I+YRA+YFG YDT KG+LP PK +VS+ IAQ VT +G++SYPFDTVRRRMMMQSG Sbjct: 185 IIYRAAYFGVYDTAKGMLPDPKNVHIIVSWMIAQSVTAVAGLVSYPFDTVRRRMMMQSGR 244 Query: 257 —AKRQYKGTLDCFVKIYQHEGISSFFRGAFSNVLRGTGGALVLVLYDKIKEF 307 A Y GTLDC+ KI + EG ++FF+GA+SNVLRG GGA VLVLYD+IK++ Sbjct: 245 KGADIMYTGTLDCWRKIAKDEGANAFFKGAWΞNVLRGMGGAFVLVLYDEIKKY 297 Pedant information for DKFZphtes3_35nl2, frame 2 Report for DKFZphtes3_35nl2.2 [LENGTH] 315 [MW] 35022.03 [pi] 9.91 [HOMOL] PIR:S37210 ADP, ATP carrier protein TI - mouse le-115 [FUNCAT] 07.16 purine and pyrimidme transporters [S. cerevisiae, YBL030c] 2e-72 [FUNCAT] 08.04 mitochondrial transport [S. cerevisiae, YBL030c] 2e-72 [ FUNCAT] 30.16 mitochondrial organization [S. cerevisiae, YBL030c] 2e-72 [FUNCAT] 01.03.19 nucleotide transport [S. cerevisiae, YBL030c] 2e-72 [FUNCAT] 01.07.10 transport of vitamins, cofactors, and prosthetic groups [S. cerevisiae, YIL006w] 2e-14 [FUNCAT] 07.99 other transport facilitators [S. cerevisiae, YIL006w] 2e-14 [FUNCAT] 01.05.07 carbohydrate transport [S. cerevisiae, YPR021c] 5e-14 [FUNCAT] 07.07 sugar and carbohydrate transporters [Ξ. cerevisiae, YPR021C] 5e-14 [FUNCAT] 07.04.07 amon transporters (cl, so4, po4, etc.) [S. cerevisiae, YKL120w] le-13 [FUNCAT] 02.13 respiration [Ξ. cerevisiae, YBR192w] 4e-13 [FUNCAT] 01.05.04 regulation of carbohydrate utilization [S. cerevisiae, YJR095w] 6e-12 [FUNCAT] 13.04 homeostasis of other ions [S. cerevisiae, YLR348c] 4e-10 [FUNCAT] 01.04.07 phosphate transport [S. cerevisiae, YLR348c] 4e-10 [FUNCAT] 01.01.07 ammo-acid transport [S. cerevisiae, YOR130C] le-06 [FUNCAT] 07.10 amino-acid transporters [S. cerevisiae, YOR130c] le-06 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YPR128c] 2e-06 [FUNCAT] 04.05.03 mrna processing (splicing) [S. cerevisiae, YKR052c] 2e-06 [BLOCKS] BL00215B Mitochondrial energy transfer proteins [BLOCKS] BL00215A Mitochondrial energy transfer proteins [PIRKW] duplication le-115 [PIRKW] phosphate transport 2e-09 [PIRKW] heart 3e-24 [PIRKW] transmembrane protein le-115 [PIRKW] mitochondrial inner membrane 7e-72 [PIRKW] transport protein 4e-08 [PIRKW] acetylated amino end le-115 [PIRKW] adipose tissue 5e-13 [PIRKW] mitochondrion le-115 [PIRKW] alternative splicing 2e-09 [PIRKW] methylated ammo acid le-115 [PIRKW] chloroplast 2e-14 [PIRKW] homodimer le-115 [SUPFAM] hypothetical protein YFR045w 3e-07 [SUPFAM] ADP, ATP carrier protein le-115 [SUPFAM] Btl protein 2e-14 [SUPFAM] ADP, ATP carrier protein repeat homology le-115 [SUPFAM] probable carrier protein YPR021c le-12 [PROSITE] MITOCH_CARRIER 3 [PFAM] Mitochondrial carrier proteins [KW] TRANSMEMBRANE 2 [KW] LOW COMPLEXITY 4.76 % SEQ MHREPAKKKAEKRLFDASSFGKDLLAGGVAAAVSKTAVAPIERVKLLLQVQASSKQISPE SEG PRD ccchhhhhhhhhhhhhchhhhhhhhhchhhhhhhhhhhcchhhhhhhhhhhhhhhhhhhh MEM SEQ ARYKGMVDCLVRIPREQGFFSFWRGNLANVIRYFPTQALNFAFKDKYKQLFMSGVNKEKQ SEG PRD hhhhhhhheeeeccccceeeeecccccceeeeecccchhhhhhhhhhhhhhccccccccc MEM SEQ FWRWFLANLASGGAAGATSLCVVYPLDFARTRLGVDIGKGPEERQFKGLGDCIMKIAKSD SEG xxxxxxxxxxxxxx PRD eeeecccccccccccceeeeeeeccchhhhhhhhhhccccchhhhhhcccceeeeeeccc MEM SEQ GIAGLYQGFGVSVQGIIVYRASYFGAYDTVKGLLPKPKKTPFLVSFFIAQVVTTCSGILS SEG PRD cccccccccceeeccceeehhhhhccccccccccccccccccchhhhhhhhhhheeeeec MEM .... MMMMMMMMMMMMMMMMMMMMMMM MMMMMMMMMMMMMMMM SEQ YPFDTVRRRMMMQSGEAKRQYKGTLDCFVKIYQHEGISSFFRGAFSNVLRGTGGALVLVL SEG PRD cccchhhhhhhhhcccceeeecccchhhhhhhhhcccccccccchhhhhccccceeeeee MEM MMMMMMMMMMM SEQ YDKIKEFFHIDIGGR SEG PRD hhhhhhheeeecccc MEM Prosite for DKFZphtes3_35nl2.2 PS00215 40->50 MITOCH_CARRIER PDOC00189 PS00215 145->155 MITOCH_CARRIER PDOC00189 PS00215 242->252 MITOCH CARRIER PDOC00189 Pfam for DKFZphtes3_35nl2.2 HMM_NAME Mitochondrial carrier proteins HMM *pFwkdFLAGGIAGmMeHTvMFPIDtIKTRMQlQgEMpM.. ahpRYkGMI +F+KD+LAGG+A+++++T+++PI+++K+++Q+Q +++ RYKGM+ Query 19 SFGKDLLAGGVAAAVSKTAVAPIERVKLLLQVQASSKQISPEARYKGMV 67 HMM dCFRwIwkNEGWRGLWRGLgANvIRYIPqWalRFGFYEFMKeMFiDyfge DC+ +I++++G++++WRG++ANVIRY+P++A++F+F++ +K +F + +++ Query 68 DCLVRIPREQGFFSFWRGNLANVIRYFPTQALNFAFKDKYKQLFMSGVNK 117 HMM ddnyWmWFwmnYMaGsmAGEwisvIitYPMWvVKTRLQaDqkHphsQp . R ++W+WF+ N+++G++AG ++S+ ++YP+++++TRL D +++++ R Query 118 EKQFWRWFLANLASGGAAG-ATSLCVVYPLDFARTRLGVD--IGKGPEER 164 HMM hYNGvWNcWrklYReEGgFkGLYRGWtPTWMRMIPYqmiYFfvYEtLKeW +++G+ +C KI +++G ++GLY+G++ +++++I+Y++ YF++Y+T K + Query 165 QFKGLGDCIMKIAKSDG-IAGLYQGFGVΞVQGIIVYRAΞYFGAYDTVKGL 213 HMM lynYtgYnPgprelCMddsPwWhWiIgWmlAGMiaWivSYPfDVVRTRMM L +++ + ++++++I++ ++ ++++I+SYPFD+VR+RMM Query 214 LP KPK—KTPFLVSFFIAQVVT-TCSGILSYPFDTVRRRMM 251 HMM Mdsm.edhkYqSmlDCWMqIYKnEGFkGFWKGFWPRIMRiMPWtAIMFml M+S+ ++++Y+++LDC+++IY++EG+ +F++G+ +++R+ ++A+++++ Query 252 MQSGEAKRQYKGTLDCFVKIYQHEGISSFFRGAFSNVLRGT-GGALVLVL 300 HMM YEqMKwFL* Y+ +K+F+ Query 301 YDKIKEFF 308 DKFZphtes3 35n24 group: testes derived DKFZphtes3_35n24 encodes a novel 365 amino acid protein without similarity to known proteins. The novel protein contains a Prosite Ig (Immunoglubulm) -MHC pattern. This pattern represents a domain, approximately one hundred amino acids long and including a conserved mtra-domain disulfide bond (llg domaini) . Thus, the novel protein is a new member of the Ig-superfamily. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . unknown complete cDNA, complete eds, EST hits Sequenced by DKFZ Locus: unknown Insert length: 1589 bp Poly A stretch at pos. 1579, polyadenylation signal at pos. 1560 1 CGATCGTCAC GTGACGCCGG GGTTCAGCGT ATCCTTGCTG GGCAACCGTC 51 TTAGAGACCA GCACTGCTGG CTGCACCATG AATGTGATCT ACCCACTGGC 101 AGTCCCCAAG GGGCGCAGAC TCTGCTGTGA GGTGTGCGAA GCCCCAGCCG 151 AGCGGGTGTG CGCGGCCTGC ACAGTCACTT ATTACTGTGG GGTGGTACAT 201 CAGAAGGCTG ACTGGGACAG CATCCATGAG AAAATATGTC AGCTCTTGAT 251 TCCACTGCGC ACTTCCATGC CCTTCTACAA TTCAGAGGAA GAACGGCAGC 301 ATGGCCTGCA GCAGCTGCAG CAGCGGCAGA AGTATTTGAT TGAATTCTGC 351 TACACCATAG CCCAGAAATA CCTCTTTGAA GGGAAACACG AAGATGCTGT 401 ACCAGCAGCT TTGCAGTCCC TTCGCTTCCG TGTGAAGCTG TATGGCCTGA 451 GCTCCGTAGA GCTTGTGCCT GCTTACCCGC TGTTGGCCGA GGCCAGCCTT 501 GGTCTGGGCC GAATCGTTCA GGCTGAAGAA TATCTATTCC AAGCCCAGTG 551 GACAGTCCTC AAATCAACTG ACTGTAGTAA TGCCACCCAC TCTTTACTGC 601 ATCGGAATCT GGGACTTCTC TATATAGCTA AGAAAAACTA TGAAGAGGCC 651 CGTTATCATC TGGCCAATGA TATTTATTTT GCCAGTTGTG CATTTGGAAC 701 AGAGGACATT AGGACTTCAG GAGGCTACTT CCACCTGGCT AATATATTCT 751 ATGACCTTAA AAAGTTGGAC CTGGCAGACA CATTGTACAC CAAGGTCTCT 801 GAGATCTGGC ATGCATATTT GAACAATCAC TATCAAGTCC TCTCACAGGC 851 TCACATCCAA CAAATGGATT TACTGGGCAA ACTATTTGAG AATGACACTG 901 GCTTGGATGA AGCCCAAGAA GCAGAAGCCA TTCGCATCCT GACTTCAATC 951 TTGAACATTC GAGAATCTAC ATCTGACAAA GCCCCCCAAA AAACCATCTT 1001 TGTTCTGAAG ATCCTGGTCA TGCTTTACTA CCTGATGATG AATTCTTCAA 1051 AGGCACAGGA ATATGGCATG AGGGCCCTCA GTCTAGCCAA AGAACAACAG 1101 CTTGATGTCC ATGAGCAAAG CACCATTCAA GAGTTATTAA GTCTCATTTC 1151 AACTGAAGAC CATCCCATTA CTTAGTGACC CATGAGCTCT GCATCAAGGG 1201 TTATTCCAGG GGCTACTGAA GATCTAATAT ATTCCAGCCT TGCACAACTG 1251 CTTTGAGGTA CTGTAGACTG CTGAAGTTTC CACCCTCTTC CCCTGGGATT 1301 GCACACATAG CTGTTATTTT TTTCTTACAC AGCATATTAA GGGAATATAA 1351 AGCTTTAGGC ATAGAAATCA CTAAAAACTG TGTTTGTCAT GACCTTTGTA 1401 CTTGATTTAT CATGACTTTG TATGACTGAG TAATATGTAG TCAGATCACT 1451 AATATGGTAT TTGTAATTAA ACTACAAATA GTTTGTCATT TCCCAGAAGT 1501 CTTCCAACGA TGCATGTTTC ATACACTTTT GCTAAAGGAG GGGTAAAGGA 1551 GGGGGTAGGG AATAAAGCTA TATTGGAACA AAAAAAAAA BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 3 ORF from 78 bp to 1172 bp; peptide length: 365 Category: putative protein Prosite motifs: IG MHC (35-42) 1 MNVIYPLAVP KGRRLCCEVC EAPAERVCAA CTVTYYCGVV HQKADWDSIH 51 EKICQLLIPL RTSMPFYNΞE EERQHGLQQL QQRQKYLIEF CYTIAQKYLF 101 EGKHEDAVPA ALQSLRFRVK LYGLSSVELV PAYPLLAEAS LGLGRIVQAE 151 EYLFQAQWTV LKSTDCSNAT HSLLHRNLGL LYIAKKNYEE ARYHLANDIY 201 FASCAFGTED IRTSGGYFHL ANIFYDLKKL DLADTLYTKV SEIWHAYLNN 251 HYQVLSQAHI QQMDLLGKLF ENDTGLDEAQ EAEAIRILTS ILNIRESTSD 301 KAPQKTIFVL KILVMLYYLM MNSSKAQEYG MRALSLAKEQ QLDVHEQSTI 351 QELLSLISTE DHPIT BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_35n24, frame 3 No Alert BLASTP hits found Pedant information for DKFZphtes3_35n24, frame 3 Report for DKFZphtes3_35n24.3 [LENGTH] 365 [MW] 41768.24 [pi] 5.82 [BLOCKS] BL00273 Heat-stable enterotoxins proteins [PROSITE] MYRISTYL 1 [PROSITE] IG_MHC 1 [PROSITE] AMIDATION 1 [PROSITE] CK2_PHOSPHO_SITE 7 [PROSITE] TYR_PHOSPHO_SITE 4 [PROSITE] PKC_PHOSPHO_SITE 3 [PROSITE] ASN_GLYCOSYLATION 3 [KW] Alpha_Beta [KW] LOW_COMPLEXITY 4.11 % SEQ MNVIYPLAVPKGRRLCCEVCEAPAERVCAACTVTYYCGVVHQKADWDSIHEKICQLLIPL SEG PRD ccceeeeeccccceeeeeeeehhhhhhhheeeeeeeeeecccccccchhhhhhhhheeec SEQ RTSMPFYNSEEERQHGLQQLQQRQKYLIEFCYTIAQKYLFEGKHEDAVPAALQSLRFRVK SEG xxxxxxxxxxxxxxx PRD cccccccchhhhhhhchhhhhhhhhhhhhhhhhhhhhhhhcccccccchhhhhhhhhhhh SEQ LYGLSSVELVPAYPLLAEASLGLGRIVQAEEYLFQAQWTVLKSTDCSNATHSLLHRNLGL SEG PRD hhccceeeeccccchhhhhccccchhhhhhhhhhhhhhhccccccccccccccccccccc SEQ LYIAKKNYEEARYHLANDIYFASCAFGTEDIRTSGGYFHLANIFYDLKKLDLADTLYTKV SEG PRD eeeehhhhhhhhhhhhhheeeeeccccccccccccceeehhhhhhhhhhhhccceeeeeh SEQ SEIWHAYLNNHYQVLSQAHIQQMDLLGKLFENDTGLDEAQEAEAIRILTSILNIRESTSD SEG PRD hhhhhhhhcccchhhhhhhhhhhhhhhhhhcccccchhhhhhhhhhhhhhhhhhhccccc SEQ KAPQKTIFVLKILVMLYYLMMNSSKAQEYGMRALSLAKEQQLDVHEQSTIQELLSLISTE SEG PRD ccccceeeehhhhhhhhhhhhcccchhhhhchhhhhhhhhhhhhhhhhhhhhhhhhhhcc SEQ DHPIT SEG PRD ccccc Prosite for DKFZphtes3_35n24.3 PS00001 168->172 ASN_GLYCOSYLATION PDOC00001 PS00001 272->276 ASN_GLYCOSYLATION PDOC00001 PS00001 322->326 ASN_GLYCOSYLATION PDOC00001 PS00005 114->117 PKC_PHOSPHO_SITE PDOC00005 PS00005 299->302 PKC_PHOSPHO_SITE PDOC00005 PS00005 323->326 PKC PHOSPHO SITE PDOC00005 PS00006 48->52 CK2 PHOSPHO SITE PDOC00006 PS00006 69->73 CK2 PHOSPHO " " SITE PDOC00006 PS00006 125->129 CK2 PHOSPHO " SITE PDOC00006 PS00006 274->278 CK2 PHOSPHO " " SITE PDOC00006 PS00006 297->301 CK2 PHOSPHO " " SITE PDOC00006 PS00006 349->353 CK2 PHOSPHO " " SITE PDOC00006 PS00006 358->362 CK2 PHOSPHO " " SITE PDOC00006 PS00007 85->93 TYR PHOSPHO " SITE PDOC00007 PS00007 186->194 TYR PHOSPHO " " SITE PDOC00007 PS00007 186->194 TYR PHOSPHO " SITE PDOC00007 PS00007 185->194 TYR PHOSPHO " " SITE PDOC00007 PS00008 275->281 MYRISTYL PDOC00008 PS00009 11->15 AMIDATION PDOC00009 PΞ00290 35->42 IG MHC PDOC00262 (No Pfam data available for DKFZphtes3_35n24.3) DKFZphtes3_35n9 group: metabolism DKFZphftes3_35n9 encodes a novel 607 amino acid protein which is a splice variant of human carboxylesterase (EC 3.1.1.1). The novel protein contains both, one carboxylesterase Bl and one B2 pattern. In comparison to EC 3.1.1.1, DKFZphtes3_35n9 shows a N-termmal extension and aa 458-474 are missing. The new protein can find application m modulation of carboxylester metabolism and as a new enzyme for biotechnologic production processes. carboxylesterase, splice variant 5' extension of mRNA and N-terminal elongation of protein (64 aa) , missing exon 1 aa 458-474 of JC5408 are missing Sequenced by DKFZ Locus : unknown Insert length: 2888 bp Poly A stretch at pos. 2878, no polyadenylation signal found 1 CTCGGCCTGA GGTGCGAGAG AAGCGGTGAC CGCGGCCCTG GCTGCTCGGA 51 CCCGGGAACA TGATGGTCGC TGGAGCAGAA GGCGCTGAGA AGGGACCACG 101 GCGGCGCTGG GTCGTGCGAG CCAGTAGCGG GCTGAAACGT AGAGGCCAGA 151 ACCAGGTCTC AGGGGGCACT AAAGGCGGTC GGAGGTAATC CCCACACCGC 201 TTCCTCCTGG AAGTCAGGCT GGCCGGGAGC TCCCGTATCC AGGACGGTTG 251 GTCGCCTCTG GCCTGGCAGG GATCCTAGTG TCTCGGGACC TCCCGGTGAC 301 GCGCCTGCCT CCCCTGCTGC ACCATAGGCC CGGGAGTACG GCGTCCCCAC 351 AGCTTGGACC GGCAGGGGCT CGTGAAATGT TTGTCAAGTG GATAAATGAC 401 CATGGCCGTG GTCTCCGCGG GAGGTGAGGA AACTGAAAGC CACCGAGGAA 451 AAGGGGGGCG CTCCTTAAGA AGTGCCGCGG TCACGTGTAC GTTTCAAAAG 501 AATGGCGTGA CTGAGTAGGG AGGGGACCGC GGAGACCCTC AGACCCTGGA 551 CTGTAAGGAG ATGAGGGGCC GTGAAGGGGA ACCCAGGAAA CTGAGTCCTG 601 AAAGCAAGGA GGAACTTCCA GAATGAAGGG CGCCGACACT CCTTCCTGCC 651 TTTGCTCAAG CGGTTCCTTC ACCCCGATCA AGTTCCTTCC CATTTCTCCA 701 TCTGGGGGAT CCTGAACGTG CACATCCTCA GAGAAGCCCT CCTGGGGTCT 751 CCAATTCTAG TTTATTGCCC CCTCCTATCG ATCCCCCAGC GCGCTCATCG 801 GGCCTGTGGA CAAGGACAGG TTTGAAGAGA GGATTCCCTG GATCGCGGAA 851 GGGCTGCAGG AATGGCACAG CCCCTTCCGA GGATGCCAAA GGAGCCCGGG 901 CAAAGGAAAG TGGCCGTGCC CGGGCCTGCC TACCACTAGA TCCCCACCCA 951 CCTATGACTG CTCAGTCCCG CTCTCCTACC ACACCCACCT TTCCCGGCCC 1001 AAGCCAGCGC ACCCCGCTGA CTCCCTGCCC AGTCCAAACT CCAAGGCTGG 1051 GCAAGGCACT GATCCACTGC TGGACAGACC CGGGGCAGCC TCTGGGTGAA 1101 CAGCAGCGTG TCCGCCGGCA GCGAACCGAG ACCAGCGAGC CGACCATGCG 1151 GCTGCACAGA CTTCGTGCGC GGCTGAGCGC GGTGGCCTGT GGGCTTCTGC 1201 TGCTTCTTGT CCGGGGCCAG GGCCAGGACT CAGCCAGTCC CATCCGGACC 1251 ACACACACGG GGCAGGTGCT GGGGAGTCTT GTCCATGTGA AGGGCGCCAA 1301 TGCCGGGGTC CAAACCTTCC TGGGAATTCC ATTTGCCAAG CCACCTCTAG 1351 GTCCGCTGCG ATTTGCACCC CCTGAGCCCC CTGAATCTTG GAGTGGTGTG 1401 AGGGATGGAA CCACCCATCC GGCCATGTGT CTACAGGACC TCACCGCAGT 1451 GGAGTCAGAG TTTCTTAGCC AGTTCAACAT GACCTTCCCT TCCGACTCCA 1501 TGTCTGAGGA CTGCCTGTAC CTCAGCATCT ACACGCCGGC CCATAGCCAT 1551 GAAGGCTCTA ACCTGCCGGT GATGGTGTGG ATCCACGGTG GTGCGCTTGT 1601 TTTTGGCATG GCTTCCTTGT ATGATGGTTC CATGCTGGCT GCCTTGGAGA 1651 ACGTGGTGGT GGTCATCATC CAGTACCGCC TGGGTGTCCT GGGCTTCTTC 1701 AGCACTGGAG ACAAGCACGC AACCGGCAAC TGGGGCTACC TGGACCAAGT 1751 GGCTGCACTA CGCTGGGTCC AGCAGAATAT CGCCCACTTT GGAGGCAACC 1801 CTGACCGTGT CACCATTTTT GGCGAGTCTG CGGGTGGCAC GAGTGTGTCT 1851 TCGCTTGTTG TGTCCCCCAT ATCCCAAGGA CTCTTCCACG GAGCCATCAT 1901 GGAGAGTGGC GTGGCCCTCC TGCCCGGCCT CATTGCCAGC TCAGCTGATG 1951 TCATCTCCAC GGTGGTGGCC AACCTGTCTG CCTGTGACCA AGTTGACTCT 2001 GAGGCCCTGG TGGGCTGCCT GCGGGGCAAG AGTAAAGAGG AGATTCTTGC 2051 AATTAACAAG CCTTTCAAGA TGATCCCCGG AGTGGTGGAT GGGGTCTTCC 2101 TGCCCAGGCA CCCCCAGGAG CTGCTGGCCT CTGCCGACTT TCAGCCTGTC 2151 CCTAGCATTG TTGGTGTCAA CAACAATGAA TTCGGCTGGC TCATCCCCAA 2201 GGTCATGAGG ATCTATGATA CCCAGAAGGA AATGGACAGA GAGGCCTCCC 2251 AGGCTGCTCT GCAGAAAATG TTAACGCTGC TGATGTTGCC TCCTACATTT 2301 GGTGACCTGC TGAGGGAGGA GTACATTGGG GACAATGGGG ATCCCCAGAC 2351 CCTCCAAGCG CAGTTCCAGG AGATGATGGC GGACTCCATG TTTGTGATCC 2401 CTGCACTCCA AGTAGCACAT TTTCAGTGTT CCCGGGCCCC TGTGTACTTC 2451 TACGAGTTCC AGCATCAGCC CAGCTGGCTC AAGAACATCA GGCCACCGCA 2501 CATGAAGGCA GACCATGTTA AATTCACTGA GGAAGAGGAG CAGCTAAGCA 2551 GGAAGATGAT GAAGTACTGG GCCAACTTTG CGAGAAATGG GAACCCCAAT 2601 GGCGAGGGTC TGCCACACTG GCCGCTGTTC GACCAGGAGG AGCAATACCT 2651 GCAGCTGAAC CTACAGCCTG CGGTGGGCCG GGCTCTGAAG GCCCACAGGC 2701 TCCAGTTCTG GAAGAAGGCG CTGCCCCAAA AGATCCAGGA GCTCGAGGAG 2751 CCTGAAGAGA GACACACAGA GCTGTAGCTC CCTGTGCCGG GGAGGAGGGG 2801 GTGGGTTCGC TGACAGGCGA GGGTCAGCCT GCTGTGCCCA CACACACCCA 2851 CTAAGGAGAA AGAAGTTGAT TCCTTCATAA AAAAAAAA BLAST Results Entry D50579 from database EMBL: Homo sapiens mRNA for carboxylesterase, complete eds. Score = 7197, P = O.Oe+00, identities = 1441/1443 Entry JC5408 from database PIR: carboxylesterase (EC 3.1.1.1) - human Score = 2808, P = 1.2e-291, identities = 542/559, positives = 543/559, frame +3 Medline entries No Medline entry Peptide information for frame 3 ORF from 954 bp to 2774 bp; peptide length: 607 Category: known protein Classification: Metabolism Prosite motifs: CARBOXYLESTERASE_B_l (279-295) CARBOXYLESTERASE B 2 (185-196) 1 MTAQSRΞPTT PTFPGPSQRT PLTPCPVQTP RLGKALIHCW TDPGQPLGEQ 51 QRVRRQRTET SEPTMRLHRL RARLSAVACG LLLLLVRGQG QDSASPIRTT 101 HTGQVLGSLV HVKGANAGVQ TFLGIPFAKP PLGPLRFAPP EPPESWSGVR 151 DGTTHPAMCL QDLTAVESEF LSQFNMTFPS DSMSEDCLYL SIYTPAHSHE 201 GSNLPVMVWI HGGALVFGMA SLYDGSMLAA LENVVVVIIQ YRLGVLGFFS 251 TGDKHATGNW GYLDQVAALR WVQQNIAHFG GNPDRVTIFG ESAGGTSVSS 301 LVVSPISQGL FHGAIMESGV ALLPGLIASS ADVISTVVAN LSACDQVDSE 351 ALVGCLRGKS KEEILAINKP FKMIPGVVDG VFLPRHPQEL LAΞADFQPVP 401 SIVGVNNNEF GWLIPKVMRI YDTQKEMDRE ASQAALQKML TLLMLPPTFG 451 DLLREEYIGD NGDPQTLQAQ FQEMMADSMF VIPALQVAHF QCSRAPVYFY 501 EFQHQPSWLK NIRPPHMKAD HVKFTEEEEQ LSRKMMKYWA NFARNGNPNG 551 EGLPHWPLFD QEEQYLQLNL QPAVGRALKA HRLQFWKKAL PQKIQELEEP 601 EERHTEL BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_35n9, frame 3 PIR:JC5408 carboxylesterase (EC 3.1.1.1) - human, N = 1, Score = 2808, P = 1.9e-292 TREMBL :HSU60553_1 gene: "hCE-2"; product: "carboxylesterase"; Human carboxylesterase (hCE-2) mRNA, complete eds., N = 1, Score = 2761, P = 1.8e-287 PIR:A34329 60K esterase (EC 3.1.1.-) isoform 2 - rabbit, N = 1, Score = 1985, P = 3.1e-205 TREMBL :D50580_1 product: "carboxylesterase precursor"; Rattus norvegicus mRNA for carboxylesterase, partial eds., N = 1, Score = 1984, P = 4e-205 >PIR:JC5408 carboxylesterase (EC 3.1.1.1) - human Length = 559 HSPs: Score = 2808 (421.3 bits), Expect = 1.9e-292, P = 1.9e-292 Identities : = 542/559 (96%), Positives = 543/559 (97%) Query: 65 MRLHRLRARLSAVACGLLLLLVRGQGQDSASPIRTTHTGQVLGSLVHVKGANAGVQTFLG 124 MRLHRLRARLSAVACGLLLLLVRGQGQDSASPIRTTHTGQVLGSLVHVKGANAGVQTFLG Sbjct: 1 MRLHRLRARLSAVACGLLLLLVRGQGQDSASPIRTTHTGQVLGΞLVHVKGANAGVQTFLG 60 Query: 125 IPFAKPPLGPLRFAPPEPPESWSGVRDGTTHPAMCLQDLTAVESEFLSQFNMTFPSDSMS 184 IPFAKPPLGPLRFAPPEPPESWSGVRDGTTHPAMCLQDLTAVESEFLSQFNMTFPΞDSMS Sbjct: 61 IPFAKPPLGPLRFAPPEPPESWSGVRDGTTHPAMCLQDLTAVESEFLSQFNMTFPSDSMS 120 Query: 185 EDCLYLSIYTPAHSHEGSNLPVMVWIHGGALVFGMASLYDGSMLAALENVVVVIIQYRLG 244 EDCLYLSIYTPAHSHEGSNLPVMVWIHGGALVFGMASLYDGSMLAALENVVVVIIQYRLG Sbjct: 121 EDCLYLSIYTPAHSHEGSNLPVMVWIHGGALVFGMASLYDGSMLAALENVVVVIIQYRLG 180 Query: 245 VLGFFΞTGDKHATGNWGYLDQVAALRWVQQNIAHFGGNPDRVTIFGEΞAGGTSVSSLVVS 304 VLGFFSTGDKHATGNWGYLDQVAALRWVQQNIAHFGGNPDRVTIFGESAGGTSVSSLVVS Sbjct: 181 VLGFFSTGDKHATGNWGYLDQVAALRWVQQNIAHFGGNPDRVTIFGESAGGTSVSSLVVS 240 Query: 305 PIΞQGLFHGAIMESGVALLPGLIASSADVISTVVANLSACDQVDSEALVGCLRGKSKEEI 364 PISQGLFHGAIMESGVALLPGLIAΞSADVISTVVANLΞACDQVDSEALVGCLRGKSKEEI Sbjct: 241 PISQGLFHGAIMESGVALLPGLIASSADVISTVVANLSACDQVDSEALVGCLRGKSKEEI 300 Query: 365 LAINKPFKMIPGVVDGVFLPRHPQELLASADFQPVPSIVGVNNNEFGWLIPKVMRIYDTQ 424 LAINKPFKMIPGVVDGVFLPRHPQELLASADFQPVPSIVGVNNNEFGWLIPKVMRIYDTQ Sbjct: 301 LAINKPFKMIPGVVDGVFLPRHPQELLASADFQPVPSIVGVNNNEFGWLIPKVMRIYDTQ 360 Query: 425 KEMDREASQAALQKMLTLLMLPPTFGDLLREEYIGDNGDPQTLQAQFQEMMADSMFVIPA 484 KEMDREASQAALQKMLTLLMLPPTFGDLLREEYIGDNGDPQTLQAQFQEMMADSMFVIPA Sbjct: 361 KEMDREASQAALQKMLTLLMLPPTFGDLLREEYIGDNGDPQTLQAQFQEMMADSMFVIPA 420 Query: 485 LQVAHFQCSRAPVYFYEFQHQPSWLKNIRPPHMKADH VKFTEEE 528 LQVAHFQCSRAPVYFYEFQHQPSWLKNIRPPHMKADH +KFTEEE Sbjct : 421 LQVAHFQCSRAPVYFYEFQHQPSWLKNIRPPHMKADHGDELPFVFRSFFGGNYIKFTEEE 480 Query: 529 EQLSRKMMKYWANFARNGNPNGEGLPHWPLFDQEEQYLQLNLQPAVGRALKAHRLQFWKK 588 EQLSRKMMKYWANFARNGNPNGEGLPHWPLFDQEEQYLQLNLQPAVGRALKAHRLQFWKK Sbjct: 481 EQLSRKMMKYWANFARNGNPNGEGLPHWPLFDQEEQYLQLNLQPAVGRALKAHRLQFWKK 540 Query: 589 ALPQKIQELEEPEERHTEL 607 ALPQKIQELEEPEERHTEL Sbjct: 541 ALPQKIQELEEPEERHTEL 559 Pedant information for DKFZphtes3_35n9, frame 3 Report for DKFZphtes3_35n9.3 (LENGTH] 607 [MW] 67051.20 [pi] 6.11 [HOMOL] PIR:JC5408 carboxylesterase (EC 3.1.1.1) - human 0.0 [BLOCKS] BL01173A Lipolytic enzymes G-D-X-G" family, histidine [BLOCKS] BL00122G [BLOCKS] BL00122F [BLOCKS] BL00122E [BLOCKS] BL00122D Carboxylesterases type-B serine proteins [BLOCKS] BL00122C Carboxylesterases type-B serine proteins [BLOCKS] BL00122B Carboxylesterases type-B serine proteins [BLOCKS] BL00122A Carboxylesterases type-B serine proteins [SCOP] dlakn 3.56.1.1.4 Bile-salt activated lipase [Bovine (Bos taurus le-156 [SCOP] d2ack 3.56.1.1.1 Acetylcholinesterase [Electric ray (Torped le-170 [SCOP] dlthg 3.56.1.9.7 type-B carboxylesterase/lipase [fungu le-149 [EC] 3.1.1.13 Sterol esterase le-52 [EC] 3.1.1.7 Acetylcholinesterase 5e-74 [EC] 3.1.1.1 Carboxylesterase 0.0 [EC] 3.1.1.8 Cholinesterase 5e-68 [EC] 3.1.1.59 Juvenile-hormone esterase le-34 [EC] 3.1.1.3 Triacylglycerol lipase 3e-52 [PIRKW] duplication 2e-47 [PIRKW] homotetramer 3e-67 [PIRKW] transmembrane protein 9e-44 [PIRKW] microsome le-130 [PIRKW] pancreas 3e-52 [PIRKW] endoplasmic reticulum le-134 [PIRKW] homotπmer le-134 [PIRKW] phosphatidylinositol linkage 5e-74 [PIRKW] synapse 3e-73 [PIRKW] liver le-131 [PIRKW] heparm binding 3e-52 [PIRKW] phosphoprotein 7e-25 [PIRKW] glycoprotein le-134 [PIRKW] thyroid hormone biosynthesis 2e-47 [PIRKW] carboxylic ester hydrolase 0.0 [PIRKW] monomer 2e-42 [PIRKW] disulfide bond 2e-31 [PIRKW] mammary gland 3e-52 [PIRKW] alternative splicing 5e-74 [PIRKW] iodine 2e-47 [PIRKW] pyroglutamic ac d 6e-39 [PIRKW] hydrolase le-135 [PIRKW] muscle 3e-73 [PIRKW] thyroid gland 2e-47 [PIRKW] membrane protein 3e-73 [PIRKW] neurotransmitter degradation 3e-73 [PIRKW] cholesterol 3e-52 [PIRKW] homodimer 2e-47 [PIRKW] nerve 3e-73 [SUPFAM] cholinesterase 0.0 [SUPFAM] triacylglycerol lipase le-32 [SUPFAM] cholinesterase homology 0.0 [SUPFAM] thyroglobulin 2e-47 [SUPFAM] thyroglobulin type I repeat homology 2e-47 [SUPFAM] juvenile-hormone esterase 2e-35 [SUPFAM] probable lipolytic protein ybaC le-07 [PROSITE] CARBOXYLESTERASE_B_2 1 [PROSITE] CARBOXYLESTERASE_B_l 1 [PFAM] Carboxylesterases [KW] Alpha_Beta [KW] 3D [KW] LOW COMPLEXITY 3.95 % SEQ MTAQSRSPTTPTFPGPSQRTPLTPCPVQTPRLGKALIHCWTDPGQPLGEQQRVRRQRTET SEG xxxxxxx ... lacj- SEQ SEPTMRLHRLRARLSAVACGLLLLLVRGQGQDSASPIRTTHTGQVLGSLVHVKGANAGVQ SEG xxxxx lacj- ETTEEEECEEEEETTEE—EE SEQ TFLGIPFAKPPLGPLRFAPPEPPESWSGVRDGTTHPAMCLQDLTAVESEFLSQFNMTFPS SEG lacj- EEEEEECEETTTGGGTTTCCEECCCCCCEEECCCCCCBCCCCCCTTTTTT-HHHHHCCCC SEQ DSMSEDCLYLSIYTPAHSHEGSNLPVMVWIHGGALVFGMASLYDGSMLAALENVVVVIIQ SEG lacj- CCBTTTTCEEEEEET—TTTTTTEEEEEEECTTTTTTCTTTTGCHHHHHHHHCCEEEECC ΞEQ YRLGVLGFFSTGDKHATGNWGYLDQVAALRWVQQNIAHFGGNPDRVTIFGESAGGTSVSS SEG lacj- CCCCGGGCCCTTTTTTTCCHHHHHHHHHHHHHHHCGGGGCEEEEEEEEEEECHHHHHHHH SEQ LVVSPISQGLFHGAIMESGVALLPGLIASSADVISTVVANLSACDQVDSEALVGCLRGKS SEG lacj- HHHCGGGTTTTCEEEEETTTTTTTTTTBCHHHHHHHHHHHHC-CCCCCHHHHHHHHHHCC SEQ KEEILAINKPFKMIPGVVDGVFLPRHPQELLASADFQPVPSIVGVNNNEFGWLIPKVMRI SEG lacj- HHHHHHHHTCCCTTTCBTTTTTTTTTHHHHHHHTTTCCCCEEEEEETBTHHHHHHTTTTT SEQ YDTQKEMDREASQAALQKMLTLLMLPPTFGDLLREEYIGDNGDPQTLQAQFQEMMADSMF SEG lacj- TTTCCCCCHHHHHHHHHHHTTTTCHHHHHHHHHHCTTTTTTTHHHH-HHHHHHHHHHHHH SEQ VIPALQVAHFQCSRAPVYFYEFQHQPSWLKNIRPPHMKADHVKFTEEEEQLSRKMMKYWA SEG lacj- HHHHHHHHHHHHCCCCEEEEEECCCCGGGTTBTTTHHHCGGGCCCHHHHHHHHHHHHHHH SEQ NFARNGNPNGEGLPHWPLFDQEEQYLQLNLQPAVGRALKAHRLQFWKKALPQKIQELEEP SEG xxxxx lacj- HHHHHCCCCCCC—CCCCBTTTTBEEEECCCCCEEETTTHHHHHHHHHHHHH SEQ EERHTEL SEG xxxxxx . Prosite for DKFZphtes3 35n9.3 PS00122 279->295 CARBOXYLESTERAΞE_B_l PDOC00112 PS00941 185->196 CARBOXYLESTERASE_B_2 PDOC00112 Pfam for DKFZphtes3_35n9.3 HMM_NAME Carboxylesterases HMM *MfMnwlιmFLLwmItWIι .WheqaprpPdPyiVdtnnCGklRGmNedtD + +L+++ ++++++++ ++Q++++P I T+ G + G ++ + Query 69 RLRARLSAVACGLLLLLVRGQGQDΞASP IRTTHT-GQVLGSLVHVK 113 HMM NG..pYYvFlGIPYAEPPVGNLRFKePQPYhePWtNVWNATnYPPMCMQW + + +FLGIP+A+PP+G LRF +P+P +E W++V++ T+ P MC+Q+ Query 114 GANAGVQTFLGIPFAKPPLGPLRFAPPEP-PESWSGVRDGTTHPAMCLQD 162 HMM ndFGFWlFdmieMWNemP.. eMSEDCLYLNVWTPWnrkPNskLPVMVWI +++ +++N++ P +MSEDCLYL+++TP+ + ++S+LPVMVWI Query 163 LTAV—ESEFLSQFNMTFPSDSMSEDCLYLSIYTPAHSHEGSNLPVMVWI 210 HMM HGGGFMFGSGhsYPliqYDgeylMMeeNVIVVtlNYRLGPFGFLSTgDid HGG+++FG + ++YDG+ L++ ENV+VV I+YRLG++GF+STGD + Query 211 HGGALVFGMA SLYDGSMLAALENVVVVIIQYRLGVLGFFSTGDKH 255 HMM lPPHGNWGLWDQRMALQWVQDNIAnFGGDPNNITIFGESAGGMSVHIHML + GNWG++DQ++AL+WVQ+NIA+FGG+P+++TIFGESAGG+SV+ ++ Query 256 AT—GNWGYLDQVAALRWVQQNIAHFGGNPDRVTIFGESAGGTSVSSLVV 303 HMM SYGGDNPPmfKqLFHRAIMQSGsAmcPWvIQsnyNaRqRAfRFAπmGCN S P + +LFH AIM+SG A+ P++I S++ + +A++ C+ Query 304 Ξ PISQGLFHGAIMESGVALLPGLIASSA—DVISTVVANLSACD 345 HMM rmDssEMIqCLRsKPwEELWdAtWnFWmWfYfPFlPWFFgPVIDGDDaPE + DS++++ CLR K+ EE++++++ +F + + +DG+ Query 346 QVDSEALVGCLRGKSKEEILAINK PFKMIPGV VDGV 381 HMM aFIPDHPeeMIkEGkFnDVPWIIGYNnDEGiWFapMmMnfnWfdEDeWId F+P+HP+E++++ F VP I+G+NN E++W++P M + + +E++ Query 382 -FLPRHPQELLASADFQPVPSIVGVNNNEFGWLIPKVMRIYDT-QKEMDR 429 HMM ltNedWyeWMPYHFYrddmsNikDMDDYiDkvyEeYPgWWDrFPqESYW ++ + ++ M +L + + + D ++EEY+G+ + PQ Query 430 EASQAALQKMLTLLMLPPT-F GDLLREEYIGDNGD-PQTLQA 469 HMM nLqDMFTDYLFWCPtRihadnHRkHwgsPVYMYeFDHPpSFGYgQFFmWR ++Q+M+ D F++P + ++H++ +PVY+YEF+H PS + Query 470 QFQEMMADΞMFVIP—ALQVAHFQCSRAPVYFYEFQHQPSW LKN 511 HMM WWPpWMgvdH* +PP+M++DH Query 512 IRPPHMKADH 521 HMM *tEEEnssMRmMMNYWINFAKhGNPNnthnglCWWPqYTsnEQYdMIMe TEEE+ +S R MM+YW+NFA++GNPN++ GL++WP ++++EQY++ + Query 525 TEEEEQLS-RKMMKYWANFARNGNPNGE—GLPHWPLFDQEEQYLQLNL 570 HMM tllmiQmCrmrDPYCNFW* + +++++ + FW Query 571 QPAVGRALKAHR—LQFW 586 DKFZphtes3_35pl7 group: testes derived DKFZphtes3_35pl7 encodes a novel 505 ammo acid protein with weak similarity to Proteins of the armadillo family. Proteins of the armadillo family are involved in diverse cellular processes in higher eukaryotes. Some of them, like armadillo, beta-catemn and plakoglobins have dual functions in intercellular junctions and signalling cascades. Others, belonging to the lmportin-alpha- subfamily are involved in NLS recognition and nuclear transport, while some members of the armadillo family have as yet unknown functions. The novel protein shows similarity to S. cerevisiae protein Yel013p (VAC8) and Danio rerio b-catenm, but contains no armadillo (arm) repeats . No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes. similarity to S. cerevisiae VAC8 complete cDNA, complete eds, few EST hits Sequenced by DKFZ Locus : unknown Insert length: 1966 bp Poly A stretch at pos. 1956, polyadenylation signal at pos. 1935 1 AAGTCAAATG TAAGATTGGT TCATTAAAAA TACTGAAGGA AATCAGTCAT 51 AATCCTCAAA TCAGACAGAA TATTGTTGAC CTTGGGGGCT TACCAATTAT 101 GGTGAATATA CTTGATTCTC CACACAAGAG TCTAAAATGT TTGGCAGCCG 151 AGACTATCGC GAATGTTGCC AAGTTTAAAA GAGCACGGCG GGTGGTGAGG 201 CAGCACGGGG GTATCACCAA ACTGGTTGCT CTACTAGACT GTGCACATGA 251 TTCCACAAAA CCTGCCCAAT CGAGTCTGTA TGAGGCCAGA GACGTGGAAG 301 TGGCTCGCTG TGGGGCACTG GCCCTGTGGA GCTGCAGTAA GAGTCATACG 351 AATAAAGAAG CCATCCGCAA AGCTGGGGGC ATTCCTCTGT TGGCTCGGCT 401 GCTGAAGACT TCTCATGAAA ACATGCTAAT TCCAGTGGTG GGGACATTGC 451 AAGAGTGTGC ATCAGAGGAA AACTACCGGG CTGCAATCAA AGCAGAAAGG 501 ATCATTGAAA ACCTTGTCAA GAACCTAAAT AGTGAGAATG AGCAGCTGCA 551 GGAGCACTGC GCCATGGCCA TTTACCAGTG TGCTGAAGAT AAGGAAACCC 601 GGGACCTCGT TAGGCTGCAC GGAGGACTTA AGCCCTTGGC CAGTCTACTC 651 AATAACACTG ACAATAAAGA GCGGTTAGCT GCTGTCACAG GGGCTATATG 701 GAAATGTTCC ATCAGCAAAG AGAATGTTAC CAAGTTTCGG GAATACAAAG 751 CCATTGAAAC CTTGGTGGGA CTTCTAACAG ATCAGCCTGA AGAAGTACTT 801 GTGAATGTGG TTGGGGCCTT GGGAGAATGC TGCCAAGAAC GTGAAAACCG 851 AGTCATTGTC CGGAAATGTG GTGGCATTCA ACCACTTGTG AACCTCCTTG 901 TTGGAATAAA CCAAGCTCTT CTTGTGAATG TTACAAAAGC AGTTGGTGCT 951 TGTGCAGTAG AACCTGAAAG TATGATGATA ATTGATCGCT TAGATGGAGT 1001 TCGTTTGTTG TGGTCCCTGC TGAAAAATCC TCACCCAGAC GTGAAGGCCA 1051 GCGCAGCATG GGCACTCTGT CCATGCATCA AAAATGCAAA GGATGCTGGG 1101 GAAATGGTTC GTTCCTTTGT TGGTGGTTTG GAACTTATTG TCAATTTACT 1151 GAAATCAGAT AACAAAGAAG TTCTGGCAAG TGTATGTGCT GCCATTACCA 1201 ACATAGCAAA AGATCAAGAA AATTTAGCTG TTATCACAGA TCATGGAGTT 1251 GTTCCTTTAT TGTCCAAACT GGCAAATACA AATAACAATA AATTGAGACA 1301 TCATCTAGCA GAAGCTATTT CACGTTGCTG TATGTGGGGC AGGAATAGAG 1351 TGGCCTTCGG TGAGCACAAA GCAGTGGCTC CACTAGTGCG TTATCTGAAA 1401 TCAAATGACA CCAACGTGCA TCGGGCGACA GCTCAGGCCT TGTACCAACT 1451 CTCAGAAGAC GCCGATAACT GCATCACCAT GCATGAGAAT GGTGCAGTAA 1501 AGCTTCTACT GGATATGGTT GGGTCCCCTG ACCAGGATCT CCAGGAAGCT 1551 GCAGCTGGTT GTATATCCAA TATCCGCAGG CTGGCTCTTG CTACAGAGAA 1601 GGCAAGATAC ACTTGAAATT TAAATGGACA TTACAAGCTA TCAAATTCTA 1651 CATGACACAG GACATGTCAC TCCCATGGCC AGAAAGCCTA AATTGGGAAA 1701 CAGTTGTTAG CAAACCCTTT CAACCATCTA AATGAAAACA CACAAATTGA 1751 AAATGCACAG AATGTTTTTC ATCTGAAAAT TGCATGGAGA CTTTTGTTTC 1801 TATTTAATGT TTTCGAGATA TGACATGTGA TAAGATGGAA AGCCAATAAA 1851 CCTGTGATAA GTTTCTAAGA ATATGAGAAT ATACGTATAT GATGTATTTT 1901 TAGTTCAGTG ATGCTTTTGT ATTTGTGGCG ATTTTAATAA AGGATATGGC 1951 CTTCCCAAAA AAAAAA BLAST Results o BLAST result Medlme entries 98413148: Yel013p (Vac8p) , an armadillo repeat protein related to plakoglobm and importin alpha is associated with the yeast vacuole membrane. 98330438: YEB3/VAC8 encodes a myristylated armadillo protein of the Saccharomyces cerevisiae vacuolar membrane that functions in vacuole fusion and inheritance. 98158703: Vac8p, a vacuolar protein with armadillo repeats, functions in both vacuole inheritance and protein targeting from the cytoplasm to vacuole . Peptide information for frame 3 ORF from 99 bp to 1613 bp; peptide length: 505 Category: similarity to known protein Classification: unset 1 MVNILDSPHK SLKCLAAETI ANVAKFKRAR RVVRQHGGIT KLVALLDCAH 51 DSTKPAQSSL YEARDVEVAR CGALALWSCS KSHTNKEAIR KAGGIPLLAR 101 LLKTSHENML IPVVGTLQEC ASEENYRAAI KAERIIENLV KNLNSENEQL 151 QEHCAMAIYQ CAEDKETRDL VRLHGGLKPL ASLLNNTDNK ERLAAVTGAI 201 WKCSISKENV TKFREYKAIE TLVGLLTDQP EEVLVNVVGA LGECCQEREN 251 RVIVRKCGGI QPLVNLLVGI NQALLVNVTK AVGACAVEPE SMMIIDRLDG 301 VRLLWSLLKN PHPDVKASAA WALCPCIKNA KDAGEMVRSF VGGLELIVNL 351 LKSDNKEVLA SVCAAITNIA KDQENLAVIT DHGVVPLLSK LANTNNNKLR 401 HHLAEAISRC CMWGRNRVAF GEHKAVAPLV RYLKSNDTNV HRATAQALYQ 451 LSEDADNCIT MHENGAVKLL LDMVGSPDQD LQEAAAGCIS NIRRLALATE 501 KARYT BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_35pl7 , frame 3 PIR:S50446 VAC8 protein - yeast (Saccharomyces cerevisiae), N = 1, Score = 237, P = 7.8e-17 PIR:T00403 T13E15.9 protein - Arabidopsis thaliana, N = 1, Score = 215, P = 4.9e-14 TREMBL: DR41081_1 product: "b-catenm"; Danio rerio b-catenin mRNA, complete eds., N = 1, Score = 195, P = 5.8e-12 >PIR:S50446 VAC8 protein - yeast (Saccharomyces cerevisiae) Length = 578 HSPs: Score = 237 (35.6 bits), Expect = 7.8e-17, P = 7.8e-17 Identities = 106/401 (26%), Positives = 177/401 (44%) Query: 92 AGGIPLLARLLKTSHENMLIPVVGTLQECASEENYRAAIKAERIIENLVKNLNSENEQLQ 151 +GG PL A +N+ + L E Y + E ++E ++ L S++ Q+Q Sbjct: 45 SGG-PLKALTTLVYSDNLNLQRSAALAFAEITEKYVRQVSRE-VLEPILILLQSQDPQIQ 102 Query: 152 EHCAMAIYQCAEDKETRDLVRLHGGLKPLASLLNNTDNKERLAAVTGAIWKCSISKENVT 211 A+ A + E + L+ GGL+PL + + DN E G I + +N Sbjct: 103 VAACAALGNLAVNNENKLLIVEMGGLEPLINQMMG-DNVEVQCNAVGCITNLATRDDNKH 161 Query: 212 KFREYKAIETLVGLLTDQPEEVLVNVVGALGECCQERENRVIVRKCGGIQPLVNLLVGIN 271 K A+ L L + V N GAL ENR + G + LV+LL + Sbjct: 162 KIATSGALIPLTKLAKSKHIRVQRNATGALLNMTHSEENRKELVNAGAVPVLVSLLSSTD 221 Query: 272 QALLVNVTKAVGACAVEPESMMIIDRLDG—VRLLWSLLKNPHPDVKASAAWALCPCIKN 329 + T A+ AV+ + + + + V L SL+ +P VK A AL + Sbjct: 222 PDVQYYCTTALSNIAVDEANRKKLAQTEPRLVSKLVSLMDSPSSRVKCQATLALRNLASD 281 Query: 330 AKDAGEMVRSFVGGLELIVNLLKSDNKE-VLASVCAAITNIAKDQENLAVITDHG V-PL 387 E+VR+ GGL +V L++SD+ VLAΞV A I NI+ N +1 D G + PL Sbjct: 282 TSYQLEIVRA—GGLPHLVKLIQSDSIPLVLASV-ACIRNISIHPLNEGLIVDAGFLKPL 338 Query: 388 LSKLANTNNNKLRHHLAEAISRCCMWG-RNRVAFGEHKAVAPLVRYLKSNDTNVHRATAQ 446 + L ++ +++ H + +NR F E AV + +V ++ Sbjct: 339 VRLLDYKDSEEIQCHAVSTLRNLAASSEKNRKEFFEΞGAVEKCKELALDSPVSV-QΞEIS 397 Query: 447 ALYQLSEDAD-NCITMHENGAVKLLLDMVGSPDQDLQEAAAGCISNI 492 A + + AD + + + E + L+ M S +Q++ AA ++N+ Sbjct: 398 ACFAILALADVSKLDLLEANILDALIPMTFSQNQEVSGNAAAALANL 444 Score = 213 (32.0 bits), Expect = 3.6e-14, P = 3.6e-14 Identities = 81/341 (23%), Positives = 163/341 (47%) Query: 163 EDKETRDLVRLHGGLKPLASLLNNTD-NKERLAAVTGAIWKCSISKENVTKFREYKAIET 221 EDK+ D G LK L +L+ + + N +R AA+ A I+++ V + + +E Sbjct: 36 EDKDQLDFYS-GGPLKALTTLVYSDNLNLQRSAALAFA EITEKYVRQVSR-EVLEP 89 Query: 222 LVGLLTDQPEEVLVNVVGALGECCQERENRVIVRKCGGIQPLVNLLVGINQALLVNVTKA 281 ++ LL Q ++ V ALG EN++++ + GG++PL+N ++G N + N Sbjct: 90 ILILLQSQDPQIQVAACAALGNLAVNNENKLLIVEMGGLEPLINQMMGDNVEVQCNAVGC 149 Query: 282 VGACAVEPESMMIIDRLDGVRLLWSLLKNPHPDVKASAAWALCPCIKNAKDAGEMVRSFV 341 + A ++ I + L L K+ H V+ +A AL + ++ E+V + Sbjct: 150 ITNLATRDDNKHKIATSGALIPLTKLAKΞKHIRVQRNATGALLNMTHSEENRKELVNA— 207 Query: 342 GGLELIVNLLKSDNKEVLASVCAAITNIAKDQENLAVI—TDHGVVPLLSKLANTNNNKL 399 G + ++V+LL S + +V A++NIA D+ N + T+ +V L L ++ ++++ Sbjct: 208 GAVPVLVSLLSSTDPDVQYYCTTALSNIAVDEANRKKLAQTEPRLVSKLVSLMDSPSΞRV 267 Query: 400 RHHLAEAISRCCMWGRNRVAFGEHKAVAPLVRYLKSNDTNVHRATAQALYQLSEDADNCI 459 + A+ ++ + LV+ ++S+ + A+ + +S N Sbjct: 268 KCQATLALRNLASDTSYQLEIVRAGGLPHLVKLIQSDSIPLVLASVACIRNISIHPLNEG 327 Query: 460 TMHENGAVKLLLDMVGSPDQDLQEAAAGCISNIRRLALATEKAR 503 + + G +K L+ ++ D + E +S +R LA ++EK R Sbjct: 328 LIVDAGFLKPLVRLLDYKDSE—EIQCHAVSTLRNLAAΞSEKNR 369 Score = 180 (27.0 bits), Expect = 1.6e-10, P = 1.6e-10 Identities = 80/346 (23%), Positives = 142/346 (41%) Query: 145 SENEQLQEHCAMAIYQCAEDKETRDLVRLHGGLKPLASLLNNTDNKERLAAVTGAIWKCΞ 204 S+N LQ A+A + E K R + R L+P+ LL + D + ++AA A+ + Sbjct: 58 SDNLNLQRSAALAFAEITE-KYVRQVSR—EVLEPILILLQSQDPQIQVAACA-ALGNLA 113 Query: 205 ISKENVTKFREYKAIETLVGLLTDQPEEVLVNVVGALGECCQERENRVIVRKCGGIQPLV 264 ++ EN E +E L+ + EV N VG + +N+ + G + PL Sbjct: 114 VNNENKLLIVEMGGLEPLINQMMGDNVEVQCNAVGCITNLATRDDNKHKIATSGALIPLT 173 Query: 265 NLLVGINQALLVNVTKAVGACAVEPESMMIIDRLDGVRLLWSLLKNPHPDVKASAAWALC 324 L + + N T A+ E+ + V +L SLL + PDV+ AL Sbjct: 174 KLAKSKHIRVQRNATGALLNMTHΞEENRKELVNAGAVPVLVSLLSSTDPDVQYYCTTALS 233 Query: 325 PCIKNAKDAGEMVRSFVGGLELIVNLLKSDNKEVLASVCAAITNIAKDQENLAVITDHGV 384 + + ++ ++ + +V+L+ S + V A+ N+A D I G Sbjct: 234 NIAVDEANRKKLAQTEPRLVSKLVSLMDSPSSRVKCQATLALRNLASDTΞYQLEIVRAGG 293 Query: 385 VPLLSKLANTNNNKLRHHLAEAISRCCMWGRNRVAFGEHKAVAPLVRYLKΞNDTNVHRAT 444 +P L KL +++ L I + N + + PLVR L D+ + Sbjct: 294 LPHLVKLIQSDSIPLVLASVACIRNISIHPLNEGLIVDAGFLKPLVRLLDYKDSEEIQCH 353 Query: 445 A-QALYQLSEDAD-NCITMHENGAVKLLLDMVGSPDQDLQEAAAGCIS 490 A L L+ ++ N E+GAV+ ++ +Q + C + Sbjct: 354 AVSTLRNLAASSEKNRKEFFESGAVEKCKELALDSPVSVQSEISACFA 401 Score = 155 (23.3 bits), Expect = 8.8e-08, P = 8.8e-08 Identities = 88/401 (21%), Positives = 175/401 (43%) Query: 60 LYEARD—VEVARCGALALWSCSKSHTNKEAIRKAGGI-PLLARLLKTSHENMLIPVVGT 116 L +++D ++VA C AL + + ++ NK I + GG+ PL+ +++ + E + VG Sbjct: 93 LLQSQDPQIQVAACAALG—NLAVNNENKLLIVEMGGLEPLINQMMGDNVE-VQCNAVGC 149 Query: 117 LQECASEENYRAAIKAERIIENLVKNLNSENEQLQEHCAMAIYQCAEDKETR-DLVRLHG 175 + A+ ++ + I + L K S++ ++Q + A+ +E R +LV G Sbjct: 150 ITNLATRDDNKHKIATΞGALIPLTKLAKSKHIRVQRNATGALLNMTHSEENRKELVNA-G 208 Query: 176 GLKPLASLLNNTDNKERLAAVTGAIWKCSISKENVTKFR—EYKAIETLVGLLTDQPEEV 233 + L SLL++TD + T A+ ++ + N K E + + LV L+ V Sbjct: 209 AVPVLVSLLΞSTDPDVQYYCTT-ALSNIAVDEANRKKLAQTEPRLVSKLVSLMDSPSSRV 267 Query: 234 LVNVVGALGECCQERENRVIVRKCGGIQPLVNLLVGINQALLVNVTKAVGACAVEPESMM 293 AL + ++ + + GG+ LV L+ + L++ + ++ P + Sbjct: 268 KCQATLALRNLASDTSYQLEIVRAGGLPHLVKLIQSDSIPLVLASVACIRNISIHPLNEG 327 Query: 294 IIDRLDGVRLLWSLLK-NPHPDVKASAAWALCPCIKNA-KDAGEMVRSFVGGLELIVNLL 351 +1 ++ L LL +++ A L ++ K+ E S G +E L Sbjct: 328 LIVDAGFLKPLVRLLDYKDΞEEIQCHAVΞTLRNLAASSEKNRKEFFES—GAVEKCKELA 385 Query: 352 KSDNKEVLA—SVCAAITNIAKDQENLAVITDHGVVPLLSKLANTNNNKLRHHLAEAISR 409 V + S C AI +A D L ++ + ++ L + + N ++ + A A++ Sbjct: 386 LDΞPVSVQSEISACFAILALA-DVSKLDLL-EANILDALIPMTFSQNQEVSGNAAAALAN 443 Query: 410 CCMWGRNRVAFGE HKAVAP-LVRYLKSNDTNVHRATAQALYQLSE 453 C N E ++ + L+R+LKS+ + QL E Sbjct: 444 LCSRVNNYTKIIEAWDRPNEGIRGFLIRFLKSDYATFEHIALWTILQLLE 493 Score = 139 (20.9 bits), Expect = 5.0e-06, P = 5.0e-06 Identities = 80/329 (24%), Positives = 142/329 (43%) Query: 37 GGITKLVALLDCAHD-STKPAQ SSLYEARDVEVARCGALALWSCSKSHTNKEAIRKA 92 G IT L D H +T A + L +++ + V R AL + + S N++ + A Sbjct: 148 GCITNLATRDDNKHKIATSGALIPLTKLAKΞKHIRVQRNATGALLNMTHSEENRKELVNA 207 Query: 93 GGIPLLARLLKTSHENMLIPVVGTLQECASEE-NYRAAIKAE-RIIENLVKNLNSENEQL 150 G +P+L LL ++ ++ L A +E N + + E R++ LV ++S + ++ Sbjct: 208 GAVPVLVSLLSSTDPDVQYYCTTALSNIAVDEANRKKLAQTEPRLVSKLVSLMDSPSSRV 267 Query: 151 QEHCAMAIYQCAEDKETR-DLVRLHGGLKPLASLLNNTDNKERLAAVTGAIWKCSISKEN 209 + +A+ A D + ++VR GGL L L+ + D+ + A I SI N Sbjct: 268 KCQATLALRNLASDTSYQLEIVRA-GGLPHLVKLIQS-DSIPLVLASVACIRNISIHPLN 325 Query: 210 VTKFREYKAIETLVGLLT-DQPEEVLVNVVGALGECCQERE-NRVIVRKCGGIQPLVNLL 267 + ++ LV LL EE+ + V L E NR + G ++ L Sbjct: 326 EGLIVDAGFLKPLVRLLDYKDSEEIQCHAVSTLRNLAASSEKNRKEFFESGAVEKCKELA 385 Query: 268 VG—INQALLVNVTKAVGACA-VEPESMMIIDRLDGVRLLWSLLKNPHPDVKASAAWA-L 323 + ++ ++ A+ A A V ++ + LD + + + +N A+AA A L Sbjct: 386 LDSPVSVQSEISACFAILALADVSKLDLLEANILDAL-IPMTFSQNQEVSGNAAAALANL 444 Query: 324 CPCIKN-AKDAGEMVRSFVGGLELIVNLLKSD 354 C + N K R G ++ LKSD Sbjct: 445 CSRVNNYTKIIEAWDRPNEGIRGFLIRFLKSD 476 Score = 136 (20.4 bits), Expect = l.le-05, P = l.le-05 Identities = 72/304 (23%), Positives = 133/304 (43%) Query: 58 SSLYEARDVEVARCGALALWSCΞKSHTNKEAIRKAGGIPLLARLLKTSHENMLIPVVGTL 117 + L +++ + V R AL + + S N++ + AG +P+L LL ++ ++ L Sbjct: 173 TKLAKSKHIRVQRNATGALLNMTHSEENRKELVNAGAVPVLVSLLSSTDPDVQYYCTTAL 232 Query: 118 QECASEE-NYRAAIKAE-RIIENLVKNLNSENEQLQEHCAMAIYQCAEDKETR-DLVRLH 174 A +E N + + E R++ LV ++S + +++ +A+ A D + ++VR Sbjct: 233 SNIAVDEANRKKLAQTEPRLVSKLVSLMDSPSSRVKCQATLALRNLASDTSYQLEIVRA- 291 Query: 175 GGLKPLASLLNNTDNKERLAAVTGAIWKCSISKENVTKFREYKAIETLVGLLT-DQPEEV 233 GGL L L+ + D+ + A I SI N + ++ LV LL EE+ Sbjct: 292 GGLPHLVKLIQS-DSIPLVLASVACIRNISIHPLNEGLIVDAGFLKPLVRLLDYKDSEEI 350 Query: 234 LVNVVGALGECCQERE-NRVIVRKCGGIQPLVNLLVG—INQALLVNVTKAVGACA-VEP 289 + V L E NR + G ++ L + ++ ++ A+ A A V Sbjct: 351 QCHAVSTLRNLAASSEKNRKEFFESGAVEKCKELALDSPVSVQSEISACFAILALADVSK 410 Query: 290 ESMMIIDRLDGVRLLWSLLKNPHPDVKAΞAAWA-LCPCIKN-AKDAGEMVRSFVGGLELI 347 ++ + LD + + + +N A+AA A LC + N K R G + Sbjct: 411 LDLLEANILDAL-IPMTFSQNQEVSGNAAAALANLCSRVNNYTKIIEAWDRPNEGIRGFL 469 Query: 348 VNLLKSD 354 + LKSD Sbjct: 470 IRFLKSD 476 Score = 114 (17.1 bits), Expect = 2.7e-03, P = 2.7e-03 Identities = 71/335 (21%), Positives = 132/335 (39%) Query: 1 MVNILDSPHKSLKCLAAETIANVAKFKRARRVVRQHGGITKLVALLDCAHDSTKPAQSSL 60 + + S H ++ A + N+ + R+ + G + LV+LL ST P Sbjct: 172 LTKLAKSKHIRVQRNATGALLNMTHSEENRKELVNAGAVPVLVSLLS STDP 222 Query: 61 YEARDVEVARCGALALWSCSKSHTNKEAIRKAGGIPLLARLLKTSHENMLIPVVGTLQEC 120 DV+ AL+ + +++ K A + + L L+ + + L+ Sbjct: 223 DVQYYCTTALSNIAVDEANRKKLAQTEPRLVSKLVSLMDSPSSRVKCQATLALRNL 278 Query: 121 ASEENYRAAIKAERIIENLVKNLNSENEQLQEHCAMAIYQCAEDKETRDLVRLHGGLKPL 180 AS+ +Y+ I + +LVK + S++ L I + L+ G LKPL Sbjct: 279 ASDTSYQLEIVRAGGLPHLVKLIQSDSIPLVLASVACIRNISIHPLNEGLIVDAGFLKPL 338 Query: 181 ASLLNNTDNKERLAAVTGAIWKCSISKE-NVTKFREYKAIETLVGLLTDQPEEVLVNVVG 239 LL+ D++E + + S E N +F E A+E L D P V + Sbjct: 339 VRLLDYKDSEEIQCHAVSTLRNLAASSEKNRKEFFESGAVEKCKELALDSPVSVQSEISA 398 Query: 240 ALGECCQERENRVIVRKCGGIQPLVNLLVGINQALLVNVTKAVG-ACAVEPESMMIIDRL 298 +++ + + + L+ + NQ + N A+ C+ 11+ Sbjct: 399 CFAILALADVSKLDLLEANILDALIPMTFSQNQEVSGNAAAALANLCSRVNNYTKIIEAW 458 Query: 299 D GVR-LLWSLLKNPHPDVKASAAWALCPCIKNAKDAGE 335 D G+R L LK+ + + A W + +++ D E Sbjct: 459 DRPNEGIRGFLIRFLKSDYATFEHIALWTILQLLESHNDKVE 500 Score = 106 (15.9 bits), Expect = 2.0e-02, P = 2.0e-02 Identitles : = 49/204 (24%), Positives = 89/204 (43%) Query: 65 DVEVARCGALA-LWSCSKSHTNKEAIRKAGGIPLLARLLKTSHENMLIPVVGTLQECA-S 122 +VEV +C A+ + + + NK I +G + L +L K+ H + G L S Sbjct: 139 NVEV-QCNAVGCITNLATRDDNKHKIATSGALIPLTKLAKSKHIRVQRNATGALLNMTHS 197 Query: 123 EENYRAAIKAERIIENLVKNLNΞENEQLQEHCAMAIYQCAEDKETRD-LVRLHGGL-KPL 180 EEN + + A + LV L+S + +Q +C A+ A D+ R L + L L Sbjct: 198 EENRKELVNAGAV-PVLVSLLSSTDPDVQYYCTTALSNIAVDEANRKKLAQTEPRLVSKL 256 Query: 181 ASLLNNTDNKERLAAVTGAIWKCSISKENVTKFREYKAIETLVGLLTDQPEEVLVNVVGA 240 SL+++ ++ + A T A+ + + + LV L+ +++ V Sbjct: 257 VSLMDSPSSRVKCQA-TLALRNLASDTSYQLEIVRAGGLPHLVKLIQSDSIPLVLASVAC 315 Query: 241 LGECCQERENRVIVRKCGGIQPLVNLL 267 + N ++ G ++PLV LL Sbjct: 316 IRNISIHPLNEGLIVDAGFLKPLVRLL 342 Pedant information for DKFZphtes3_35pl7, frame 3 Report for DKFZphtes3_35pl7.3 [LENGTH] 505 [MW] 55224.34 [pi] 8.43 [HOMOL] PIR:S50446 VAC8 protein - yeast (Saccharomyces cerevisiae) 2e-16 [FUNCAT] 30.25 vacuolar and lysosomal organization [S. cerevisiae, YEL013w] 8e-18 [FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YEL013w] 8e-18 [FUNCAT] 09.25 vacuolar and lysosomal biogenesis [S. cerevisiae, YEL013w] 8e-18 [FUNCAT] 08.01 nuclear transport [S. cerevisiae, YNL189w] 3e-06 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YNL189w] 3e-06 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YNL189w] 3e-06 [BLOCKS] BL01265C [BLOCKS] BL00242A Integrins alpha chain proteins [SCOP] d3bct 1.91.1.1.1 beta-Catemn [Mouse (Mus musculus) 7e-18 [PIRKW] cytosol 3e-ll [PIRKW] apoptosis 3e-ll [PIRKW] carcinogenesis 3e-ll [PIRKW] cell adhesion 3e-ll [PIRKW] cytoskeleton 3e-12 [SUPFAM] pendulm le-07 [KW] All_Alpha [KW] 3D [KW] LOW COMPLEXITY 2.38 % SEQ MVNILDSPHKSLKCLAAETIANVAKFKRARRVVRQHGGITKLVALLDCAHDSTKPAQSSL SEG xxxxxxxxxxxx 2bct- HH SEQ YEARDVEVARCGALALWSCSKSHTNKEAIRKAGGIPLLARLLKTSHENMLIPVVGTLQEC SEG 2bct- HHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHGGGCCCHHHHHHHHHHHHHH SEQ ASEENYRAAIKAERIIENLVKNLNSENEQLQEHCAMAIYQCAEDKETRDLVRLHGGLKPL SEG 2bct- HHTTTHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHTTHHHHHHHHHHCHHHHH SEQ ASLLNNTDNKERLAAVTGAIWKCSISKENVTKFREYKAIETLVGLLTDQPEEVLVNVVGA SEG 2bct- HHHHH-HCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHTTTTTCCHHHHHHHHHH SEQ LGECCQERENRVIVRKCGGIQPLVNLLVGINQALLVNVTKAVGACAVEPESMMIIDRLDG SEG 2bct- H HHHHHCCCCTTTHHHHHHHHHHHHCTTTHHHHHHHHHTTTHHHHHHHH-HHCH SEQ VRLLWSLLKNPHPDVKASAAWALCPCIKNAKDAGEMVRSFVGGLELIVNLLKSDNKEVLA SEG 2bct- HHHHHHHHHTTTHHHHHHHHHHHHHHHCCCCHH-HHHHHHHHHHHHHHHHCTTTTTHHHH SEQ SVCAAITNIAKDQENLAVITDHGVVPLLSKLANTNNNKLRHHLAEAISRCCMWGRNRVAF SEG 2bct- HHHHHHHHHHHCGGGHHHHHHHCHHHHHHHHHHHHHHTTTCCHHHHHHHHHHHHCHHHHH SEQ GEHKAVAPLVRYLKSNDTNVHRATAQALYQLSEDADNCITMHENGAVKLLLDMVGSPDQD SEG 2bct- HTTTHHHHHHHHHCCCCHHHHHHHHHHHHHHHTTHHHHHHHHHCCHHHHHHHTTTTTTHH SEQ LQEAAAGCISNIRRLALATEKARYT SEG 2bct- HHHHHHHHH (No Prosite data available for DKFZphtes3_35pl7.3) (No Pfam data available for DKFZphtes3_35pl7.3) DKFZphtes3_35p22 group: cell cycle DKFZphtes3_35p22 encodes a novel 549 amino acid protein, with similarity to oncogene 1 (tre-2 locus) . The novel protein is closely raleted to human tre-2 and other enzymes involved in the degradation of ubiquitinated proteins. The human tre-2 oncogene encodes a deubiquitmating enzyme, indicating a role for the ubiquitm system in mammalian growth control. The novel protein can find application in cancer diagnostics and treatment, and in regulating protein stability and growth control via regulation of ubiquitination. strong similarity to oncogene 1 (tre-2 locus) membrane regions: 1 complete cDNA, complete eds, EST hits Sequenced by DKFZ Locus: map="17" Insert length: 2072 bp Poly A stretch at pos. 2062, polyadenylation signal at pos. 2039 1 GTTACACACA GGCAGTGGTA TCTGTGAGCA GCTCTGTGGA CTCAAAGGTT 51 TTCTCCCTGA GAGGCATGAC CCAGGCCAGC TGATTCATCA GAATCAGGAT 101 GGACGTGGTA GAGGTCGCGG GCAGTTGGTG GGCACAAGAG CGAGAGGACA 151 TCATTATGAA ATACGAAAAG GGACACCGAG CTGGGCTGCC AGAGGACAAG 201 GGGCCTAAGC CTTTTCGAAG CTACAACAAC AACGTCGATC ATTTGGGGAT 251 TGTACATGAG ACGGAGCTGC CTCCTCTGAC TGCGCGGGAG GCGAAGCAAA 301 TTCGGCGGGA GATCAGCCGA AAGAGCAAGT GGGTGGATAT GCTGGGAGAC 351 TGGGAGAAAT ACAAAAGCAG CAGAAAGCTC ATAGATCGAG CGTACAAGGG 401 AATGCCCATG AACATCCGGG GCCCGATGTG GTCAGTCCTC CTGAACACTG 451 AGGAAATGAA GTTGAAAAAC CCCGGAAGAT ACCAGATCAT GAAGGAGAAG 501 GGCAAGAAGT CATCTGAGCA CATCCAGCGC ATCGACCGGG ACGTAAGCGG 551 GACATTAAGG AAGCATATAT TCTTCAGGGA TCGATACGGA ACCAAGCAGC 601 GGGAACTACT CCACATCCTC CTGGCATATG AGGAGTACAA CCCGGAGGTG 651 GGCTACTGCA GGGACCTGAG CCACATCGCC GCCTTGTTCC TCCTCTATCT 701 TCCTGAGGAG GATGCATTCT GGGCACTGGT GCAGCTGCTG GCCAGTGAGA 751 GGCACTCCCT GCAGGGATTT CACAGCCCAA ATGGCGGGAC CGTCCAGGGG 801 CTCCAAGACC AACAGGAGCA TGTGGTAGCC ACGTCACAAC CCAAGACCAT 851 GGGGCATCAG GACAAGAAAG ATCTATGTGG GCAGTGTTCC CCGTTAGGCT 901 GCCTCATCCG GATATTGATT GACGGGATCT CTCTCGGGCT CACCCTGCGC 951 CTGTGGGACG TGTATCTGGT AGAAGGCGAA CAGGCGCTGA TGCCGATAAC 1001 AAGAATCGCC TTTAAGGTTC AGCAGAAGCG CCTCACGAAG ACGTCCAGGT 1051 GTGGCCCGTG GGCACGTTTT TGCAACCGGT TCGTTGATAC CTGGGCCAGG 1101 GATGAGGACA CTGTGCTCAA GCATCTTAGG GCCTCTATGA AGAAACTAAC 1151 AAGAAAGAAG GGGGACCTGC CACCCCCAGC CAAACCCGAG CAAGGGTCGT 1201 CGGCATCCAG GCCTGTGCCG GCTTCACGTG GCGGGAAGAC CCTCTGCAAG 1251 GGGGACAGGC AGGCCCCTCC AGGCCCACCA GCCCGGTTCC CGCGGCCCAT 1301 TTGGTCAGCT TCCCCGCCAC GGGCACCTCG TTCTTCCACA CCCTGTCCTG 1351 GTGGGGCTGT CCGGGAAGAC ACCTACCCTG TGGGCACTCA GGGTGTGCCC 1401 AGCCCGGCCC TGGCTCAGGG AGGACCTCAG GGTTCCTGGA GATTCCTGCA 1451 GTGGAACTCC ATGCCCCGCC TCCCAACGGA CCTGGACGTA GAGGGCCCTT 1501 GGTTCCGCCA TTATGATTTC AGACAGAGCT GCTGGGTCCG TGCCATATCC 1551 CAGGAGGACC AGCTGGCCCC CTGCTGGCAG GCTGAACACC CTGCGGAGCG 1601 GGTGAGATCG GCTTTCGCTG CACCCAGCAC TGATTCCGAC CAGGGCACCC 1651 CCTTCAGAGC TAGGGACGAA CAGCAGTGTG CTCCCACCTC AGGGCCTTGC 1701 CTCTGCGGCC TCCACTTGGA AAGTTCTCAG TTCCCTCCAG GCTTCTAGAA 1751 GCATCTGGGC CAGGGCTCAT GGCTGGATAA TTTCCCTAGG CTTAACAACC 1801 CAAGCAAGCT TCGCATCCTC GTTTTATTTT TGGTTAAACT TATGAAAATG 1851 TATTAAGAAA GAGTGCAGCT CGAGAGAGAT TCAGAGATGG AACACACCAG 1901 ACCCCAGATC ACAAAGCCAA CCATGCCCAG CCCCTCCCAG CACCCCCAGC 1951 CCCACGACCA TCGTTCTGAA TTCTGACGAC ACCGTGAGCC TGCCTTTGTA 2001 CTTCAAACTC ATGGAAGGAT AACCACCTTC ATGTTTTGAA ATAAATGTTT 2051 CCTGTTGAAA TGAAAAAAAA AA BLAST Results Entry AC003976 from database EMBL: Homo sapiens chromosome 17, clone hCIT.91_J_4, complete sequence. Score = 4385, P = O.Oe+00, identities = 881/886 14 exons Entry HSG19723 from database EMBL: human STS A001W35. Score = 850, P = 1.9e-32, identities = 170/170 Medline entries 92228503: A novel transcriptional unit of the tre oncogene widely expressed in human cancer cells. 94067315: The yeast DOA4 gene encodes a deubiquitmating enzyme related to a product of the human tre-2 oncogene. 95176708: UBP5 encodes a putative yeast ubiquitin-specific protease that is related to the human Tre-2 oncogene product. Peptide information for frame 3 ORF from 99 bp to 1745 bp; peptide length: 549 Category: strong similarity to known protein 1 MDWEVAGSW WAQEREDIIM KYEKGHRAGL PEDKGPKPFR SYNNNVDHLG 51 IVHETELPPL TAREAKQIRR EISRKSKWVD MLGDWEKYKS SRKLIDRAYK 101 GMPMNIRGPM WSVLLNTEEM KLKNPGRYQI MKEKGKKSSE HIQRIDRDVS 151 GTLRKHIFFR DRYGTKQREL LHILLAYEEY NPEVGYCRDL SHIAALFLLY 201 LPEEDAFWAL VQLLASERHS LQGFHSPNGG TVQGLQDQQE HVVATSQPKT 251 MGHQDKKDLC GQCSPLGCLI RILIDGISLG LTLRLWDVYL VEGEQALMPI 301 TRIAFKVQQK RLTKTSRCGP WARFCNRFVD TWARDEDTVL KHLRASMKKL 351 TRKKGDLPPP AKPEQGSSAS RPVPASRGGK TLCKGDRQAP PGPPARFPRP 401 IWSASPPRAP RSSTPCPGGA VREDTYPVGT QGVPSPALAQ GGPQGSWRFL 451 QWNSMPRLPT DLDVEGPWFR HYDFRQSCWV RAISQEDQLA PCWQAEHPAE 501 RVRSAFAAPS TDSDQGTPFR ARDEQQCAPT SGPCLCGLHL ESSQFPPGF BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_35p22, frame 3 PIR:Ξ22155 oncogene 1 (tre-2 locus) (clone 210) - human, N = 1, Score = 2181, P = 5.5e-226 PIR:S57867 oncogene 1 - human, N = 1, Score = 1536, P = 1.2e-157 >PIR:S22155 oncogene 1 (tre-2 locus) (clone 210) - human Length = 786 HSPs: Score = 2181 (327.2 bits), Expect = 5.5e-226, P = 5.5e-226 Identities = 405/500 (81%), Positives = 440/500 (88%) Query: 1 MDVVEVAGSWWAQEREDIIMKYEKGHRAGLPEDKGPKPFRSYNNNVDHLGIVHETELPPL 60 MD+VE A S AQER+DI+MKY+KGHRAGLPEDKGP+P N+++D GI+HETELPP+ Sb j ct: 1 MDMVENADSLQAQERKDILMKYDKGHRAGLPEDKGPEPV-GINΞSIDRFGILHETELPPV 59 Query: 61 TAREAKQIRREISRKSKWVDMLGDWEKYKSSRKLIDRAYKGMPMNIRGPMWSVLLNTEEM 120 TAREAK+IRRE++R SKW++MLG+WE YK S KLIDR YKG+PMNIRGP+WSVLLN +E+ Sb j ct: 60 TAREAKKIRREMTRTSKWMEMLGEWETYKHSSKLIDRVYKGIPMNIRGPVWSVLLNIQEI 119 Query: 121 KLKNPGRYQIMKEKGKKSSEHIQRIDRDVSGTLRKHIFFRDRYGTKQRELLHILLAYEEY 180 KLKNPGRYQIMKE+GK+SΞEHI ID DV TLR H+FFRDRYG KQREL +ILLAY EY Sb j ct: 120 KLKNPGRYQIMKERGKRSSEHIHHIDLDVRTTLRNHVFFRDRYGAKQRELFYILLAYSEY 179 Query: 181 NPEVGYCRDLSHIAALFLLYLPEEDAFWALVQLLASERHSLQGFHSPNGGTVQGLQDQQE 240 NPEVGYCRDLSHI ALFLLYLPEEDAFWALVQLLASERHSL GFHSPNGGTVQGLQDQQE Sb j ct: 180 NPEVGYCRDLSHITALFLLYLPEEDAFWALVQLLASERHSLPGFHSPNGGTVQGLQDQQE 239 Query: 241 HVVATSQPKTMGHQDKKDLCGQCSPLGCLIRILIDGISLGLTLRLWDVYLVEGEQALMPI 300 HVV SQPKTM HQDK+ LCGQC+ LGCL+R LIDGISLGLTLRLWDVYLVEGEQ LMPI Sb j ct: 240 HVVPKSQPKTMWHQDKEGLCGQCASLGCLLRNLIDGISLGLTLRLWDVYLVEGEQVLMPI 299 Query: 301 TRIAFKVQQKRLTKTΞRCGPWARFCNRFVDTWARDEDTVLKHLRASMKKLTRKKGDLPPP 360 T IA KVQQKRL KTSRCG WAR N+F DTWA ++DTVLKHLRAS KKLTRK+GDLPPP Sb j ct: 300 TSIALKVQQKRLMKTSRCGLWARLRNQFFDTWAMNDDTVLKHLRASTKKLTRKQGDLPPP 359 Query: 361 AKPEQGSSASRPVPASRGGKTLCKGDRQAPPGPPARFPRPIWSASPPRAPRSSTPCPGGA 420 AK EQGS A RPVPASRGGKTLCKG RQAPPGPPA+F RPI SASPP A R STPCPGGA Sbjct: 360 AKREQGSLAPRPVPASRGGKTLCKGYRQAPPGPPAQFQRPICSASPPWASRFSTPCPGGA 419 Query: 421 VREDTYPVGTQGVPSPALAQGGPQGSWRFLQWNSMPRLPTDLDVEGPWFRHYDFRQSCWV 480 VREDTYPVGTQGVPS ALAQGGPQGSWRFL+W SMPRLPTDLD+ GPWF HYDF +SCWV Sbjct: 420 VREDTYPVGTQGVPSLALAQGGPQGSWRFLEWKΞMPRLPTDLDIGGPWFPHYDFERSCWV 479 Query: 481 RAISQEDQLAPCWQAEHPAE 500 RAISQEDQLA CWQAEH E Sbjct: 480 RAISQEDQLATCWQAEHCGE 499 Pedant information for DKFZphtes3_35p22, frame 3 Report for DKFZphtes3_35p22.3 [LENGTH] 549 [MW] 62159.16 [pi] 9.23 [HOMOL] PIR:S22155 oncogene 1 (tre-2 locus) (clone 210) - human 0.0 [FUNCAT] 11.01 stress response [S. cerevisiae, YGRlOOw] 2e-16 [FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YGRlOOw] 2e-16 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YNL293w] 3e-15 [PIRKW] transmembrane protein 6e-14 [PROSITE] MYRISTYL 6 [PROSITE] AMIDATION 1 [PROSITE] CAMP_PHOSPHO_SITE 3 [PROSITE] CK2_PHOSPHO_SITE 4 [PROSITE] TYR_PHOSPHO_SITE 2 [PROSITE] PKC_PHOSPHO_SITE 10 [KW] TRANSMEMBRANE 1 [KW] LOW COMPLEXITY 5.28 % SEQ MDVVEVAGSWWAQEREDIIMKYEKGHRAGLPEDKGPKPFRSYNNNVDHLGIVHETELPPL SEG PRD ccceeeccchhhhhhhhhhhhhhccccccccccccccceeeeeccccccccccccccccc MEM SEQ TAREAKQIRREISRKSKWVDMLGDWEKYKSSRKLIDRAYKGMPMNIRGPMWSVLLNTEEM SEG PRD chhhhhhhhhhhhhhhhhhhhhcccchhhhhhhhhhhhhhcccccccccceeeccccccc MEM SEQ KLKNPGRYQIMKEKGKKSSEHIQRIDRDVSGTLRKHIFFRDRYGTKQRELLHILLAYEEY SEG PRD ccccccchhhhhhhccccchhhhhhhhhhhhccccccccccccccchhhhhhhhhhhhhc MEM SEQ NPEVGYCRDLSHIAALFLLYLPEEDAFWALVQLLASERHSLQGFHSPNGGTVQGLQDQQE SEG PRD ccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccccccchhhhhhhhhh MEM SEQ HVVATSQPKTMGHQDKKDLCGQCSPLGCLIRILIDGISLGLTLRLWDVYLVEGEQALMPI SEG PRD hhhhhhhchhhhhhhhccccccccchhhhhhhhhhccccchhhhhhhhhccccceeeehh MEM MMMMMMMMMMMMMMMMMM SEQ TRIAFKVQQKRLTKTSRCGPWARFCNRFVDTWARDEDTVLKHLRASMKKLTRKKGDLPPP SEG PRD hhhhhhhhhhhhhhhcccchhhhhhhhhhhhcccccchhhhhhhhhhhhhhhhhhhcccc MEM SEQ AKPEQGSSASRPVPASRGGKTLCKGDRQAPPGPPARFPRPIWSASPPRAPRSSTPCPGGA SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxx ... PRD ccccccccccccccccccceeeeccccccccccccccccccccccccccccccccccccc MEM SEQ VREDTYPVGTQGVPSPALAQGGPQGSWRFLQWNSMPRLPTDLDVEGPWFRHYDFRQSCWV SEG PRD cccccccccccccccccccccccccceeeeeccccccccccccccccccccccccccccc MEM SEQ RAISQEDQLAPCWQAEHPAERVRSAFAAPSTDSDQGTPFRARDEQQCAPTSGPCLCGLHL SEG PRD cchhhhhhhhhhhhhhcchhhhhhhhccccccccccccccchhhhhcccccccccceeee MEM SEQ ESSQFPPGF SEG PRD ccccccccc MEM Prosite for DKFZphtes3_35p22.3 PS00004 136->140 CAMP_PHOSPHO_SITE PDOC00004 PS00004 310->314 CAMP_PHOSPHO_SITE PDOC00004 PS00004 348->352 CAMP_PHOSPHO_SITE PDOC00004 PS00005 61->64 PKC_PHOSPHO_SITE PDOC00005 PS00005 73->76 PKC_PHOSPHO_SITE PDOC00005 PS00005 90->93 PKC_PHOSPHO_SITE PDOC00005 PS00005 152->155 PKC_PHOSPHO_SITE PDOC00005 PS00005 216->219 PKC_PHOSPHO_SITE PDOC00005 PS00005 282->285 PKC_PHOSPHO_SITE PDOC00005 PS00005 315->318 PKC_PHOSPHO_SITE PDOC00005 PS00005 346->349 PKC_PHOSPHO_SITE PDOC00005 PS00005 351->354 PKC_PHOSPHO_SITE PDOC00005 PS00005 446->449 PKC_PHOSPHO_SITE PDOC00005 PS00006 61->65 CK2_PHOSPHO_SITE PDOC00006 PS00006 460->464 CK2_PHOSPHO_SITE PDOC00006 PS00006 484->488 CK2_PHOSPHO_SITE PDOC00006 PS00006 511->515 CK2_PHOSPHO_SITE PDOC00006 PS00007 93->100 TYR_PHOSPHO_SITE PDOC00007 PS00007 92->100 TYR_PHOΞPHO_SITE PDOC00007 PS00008 8->14 MYRISTYL PDOC00008 PS00008 101->107 MYRISTYL PDOC00008 PS00008 230->236 MYRISTYL PDOC00008 PS00008 276->282 MYRISTYL PDOC00008 PS00008 366->372 MYRISTYL PDOC00008 PS00008 441->447 MYRISTYL PDOC00008 PS00009 134->138 AMIDATION PDOC00009 (No Pfam data available for DKFZphtes3_35p22.3) DKFZphtes3_4b4 group: testes derived DKFZphtes3_4b4 encodes a novel 497 ammo acid protein similar to SCP proteins and a human trypsin inhibitor. The novel protein contains an extracellular proteins SCP/Tpx-l/Ag5/PR-l/Sc7 signature 2, predicted by Prosite and Pfam. This domain is found in a variety of extracellular proteins from eukaryotes that have been found to be evolutionary related. The exact function of these proteins is not yet known. In addition, the protein is similar to a human trypsin inhibitor. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application m studying the expression profile of testis-specific genes or as a new protease inhibitor. strong similarity to trypsin inhibitor might be a new protease inhibitor 7 Sequenced by AGOWA Locus: /map="333.4 cR from top of Chrl6 linkage group" Insert length: 4574 bp Poly A stretch at pos. 4551, polyadenylation signal at pos. 4539 1 GGCGGCTGCT CCCATTGAGC TGTCTGCTCG CTGTGCCCGC TGTGCCTGCT 51 GTGCCCGCGC TGTCGCCGCT GCTACCGCGT CTGCTGGACG CGGGAGACGC 101 CAGCGAGCTG GTGATTGGAG CCCTGCGGAG AGCTCAAGCG CCCAGCTCTG 151 CCCGAGGAGC CCAGGCTGCC CCGTGAGTCC CATAGTTGCT GCAGGAGTGG 201 AGCCATGAGC TGCGTCCTGG GTGGTGTCAT CCCCTTGGGG CTGCTGTTCC 251 TGGTCTGCGG ATCCCAAGGC TACCTCCTGC CCAACGTCAC TCTCTTAGAG 301 GAGCTGCTCA GCAAATACCA GCACAACGAG TCTCACTCCC GGGTCCGCAG 351 AGCCATCCCC AGGGAGGACA AGGAGGAGAT CCTCATGCTG CACAACAAGC 401 TTCGGGGCCA GGTGCAGCCT CAGGCCTCCA ACATGGAGTA CATGACCTGG 451 GATGACGAAC TGGAGAAGTC TGCTGCAGCG TGGGCCAGTC AGTGCATCTG 501 GGAGCACGGG CCCACCAGTC TGCTGGTGTC CATCGGGCAG AACCTGGGCG 551 CTCACTGGGG CAGGTATCGC TCTCCGGGGT TCCATGTGCA GTCCTGGTAT 601 GACGAGGTGA AGGACTACAC CTACCCCTAC CCGAGCGAGT GCAACCCCTG 651 GTGTCCAGAG AGGTGCTCGG GGCCTATGTG CACGCACTAC ACACAGATAG 701 TTTGGGCCAC CACCAACAAG ATCGGTTGTG CTGTGAACAC CTGCCGGAAG 751 ATGACTGTCT GGGGAGAAGT TTGGGAGAAC GCGGTCTACT TTGTCTGCAA 801 TTATTCTCCA AAGGGGAACT GGATTGGAGA AGCCCCCTAC AAGAATGGCC 851 GGCCCTGCTC TGAGTGCCCA CCCAGCTATG GAGGCAGCTG CAGGAACAAC 901 TTGTGTTACC GAGAAGAAAC CTACACTCCA AAACCTGAAA CGGACGAGAT 951 GAATGAGGTG GAAACGGCTC CCATTCCTGA AGAAAACCAT GTTTGGCTCC 1001 AACCGAGGGT GATGAGACCC ACCAAGCCCA AGAAAACCTC TGCGGTCAAC 1051 TACATGACCC AAGTCGTCAG ATGTGACACC AAGATGAAGG ACAGGTGCAA 1101 AGGGTCCACG TGTAACAGGT ACCAGTGCCC AGCAGGCTGC CTGAACCACA 1151 AGGCGAAGAT CTTTGGAACT CTGTTCTATG AAAGCTCGTC TAGCATATGC 1201 CGCGCCGCCA TCCACTACGG GATCCTGGAT GACAAGGGAG GCCTGGTGGA 1251 TATCACCAGG AACGGGAAGG TCCCCTTCTT CGTGAAGTCT GAGAGACACG 1301 GCGTGCAGTC CCTCAGCAAA TACAAACCTT CCAGCTCATT CATGGTGTCA 1351 AAAGTGAAAG TGCAGGATTT GGACTGCTAC ACGACCGTTG CTCAGCTGTG 1401 CCCGTTTGAA AAGCCAGCAA CTCACTGCCC AAGAATCCAT TGTCCGGCAC 1451 ACTGCAAAGA CGAACCTTCC TACTGGGCTC CGGTGTTTGG AACCAACATC 1501 TATGCAGATA CCTCAAGCAT CTGCAAGACA GCCGTGCACG CGGGAGTCAT 1551 CAGCAACGAG AGTGGGGGTG ACGTGGACGT GATGCCCGTG GATAAAAAGA 1601 AGACCTACGT GGGCTCGCTC AGGAATGGAG TTCAGTCTGA AAGCCTGGGG 1651 ACTCCTCGGG ATGGAAAGGC CTTCCGGATC TTTGCTGTCA GGCAGTGAAT 1701 TTCCAGCACC AGGGGAGAAG GGGCGTCTTC AGGAGGGCTT CGGGGTTTTG 1751 CTTTTATTTT TATTTTGTCA TTGCGGGGTA TATGGAGAGT CAGGAAACTT 1801 CCTTTGACTG ATGTTCAGTG TCCATCACTT TGTGGCCTGT GGGTGAGGTG 1851 ACATCTCATC CCCTCACTGA AGCAACAGCA TCCCAAGGTG CTCAGCCGGA 1901 CTCCCTGGTG CCTGATCCTG CTGGGGCCCG GGGGTCTCCA TCTGGACGTC 1951 CTCTCTCCTT TAGAGATCTG AGCTGTCTCT TAAAGGGGAC AGTTGCCCAA 2001 AATGTTCCTT GCTATGTGTT CTTCTGTTGG TGGAGGAAGT TGATTTCAAC 2051 CTCCCTGCCA AAAGAACAAA CCATTTGAAG CTCACAATTG TGAAGCATTC 2101 ACGGCGTCGG AAGAGGCCTT TTGAGCAAGC GCCAATGAGT TTCAGGAATG 2151 AAGTAGAAGG TAGTTATTTA AAAATAAAAA ACACAGTCCG TCCCTACCAA 2201 TAGAGGAAAA TGGTTTTAAT GTTTGCTGGT CAGACAGACA AATGGGCTAG 2251 AGTAAGAGGG CTGCGGGTAT GAGAGACCCC GGCTCCGCCC TGGCACGTGT 2301 CCTTGCTGGC GGCCCGCCAC AGGCCCCCTT CAATGGCCGC ATTCAGGATG 2351 GCTCTATACA CAGCAGTGCT GGTTTATGTA GAGTTCAGCA GTCACTTCAG 2401 AGATGTATCT TGTCTTTGTC AGGCCCTTCA TCTTCATGGC CCACCTGTTT 2451 TCTGCCGTGA CCTTTGGTCC CATTGAGGAC TAAGGATCGG GACCCTTTCT 2501 TTACCCCCTA CCCATTGTGG CTCCCACCCT GCCTCGGACT GGTTTACGTG 2551 TCCTGGTTCA CACCCAGGAC TTTTCTTTGC AAGCGAACCT GTTTGAAGCC 2601 CAAGTCTTAA CTCCTGGTCT CGTAAGGTTC CACTGAGACG AGATGTCTGA 2651 GAACAACCAA AGAAGGCCTG CTCTTTGCTG CTTTTAAAAA ATGACAATTA 2701 AATGTGCAGA TTCCCCACGC ACCCGATGAC CTATTTTTTC AGCCGTGGGA 2751 GGAATGGAGT CTTTGGTACA TTCCTCACCG AGGTTAGCAG CTCAGTTTGT 2801 GGTTATGAAA CCGTCTGTGG CCTCATGACA GCGAGAGATG GGAATACACT 2851 AGAAGGATCT CTTTTCCTGT TTTCGTGAAA CGACTCTTGC CAAACGTTCC 2901 CGAGGCGCCA AGGAGTGTAG TACACCCTGG CTGCCATCAC TCTATAAAAG 2951 TGCTTCATGA GCCCAGACCA AAAGCCCACA GTGAAATGAA GTACCCTTTT 3001 GTAAATAGCA TTTTTTTGCA GAAGGTGAAA ATTCCACTCT CTACCACCGG 3051 GCCAGCCAAT AGATCACTTT GGTGAATGCT AGTTTCAAAT TTGATTCAAA 3101 ATATTTCTTA GGTGAAAGAA CTAGCAGAAA GTCAAAAACT AAGATACTGT 3151 AGACTGGACA AGAAATTCTA CCTGGGCACC TAGGTGATGC CTTCTTTCTT 3201 TGATTGCCTT TCTAATAAAT GCAGAATCTG AAGGTAAATA GGTTTAAAAC 3251 AAAACAAAAA CCCACCCCTT TAAGGAGTTG GTAAAAAGCA GTTCAACTCT 3301 TAGCTTGACT GAGCTAAAAT TCACAGGACT ACGTGCTTTG TGCATTGTAG 3351 TCTAGTCGTA ATTCATAGGT ACTGACTCCT CAGCCCCAAA TGTCGGAGAG 3401 GAAGAATTCG GTCAGCCTGT CAGGTCGTGA GTCCAGTTAC CACCAAACAT 3451 CTGGGAAACT TCTGGGTGCT GGGTGCTCTG CTGCTGGACT TTTGTGGCTG 3501 TGTCTGTGTC TGCAAGATAA ATTAGATCGC CCTGTGGGGT TTGCAGAATT 3551 AGTGAAGGGT CCAGGACGAT CCCAGTGGGC TCGCTTCCAA AGCATCCCAC 3601 TCAAGGGAGA CTTGAAACTT CCAGTGTGAG TTGACCCCAT CATTTAAAAA 3651 TAAAGTCCCC GGGTTCCTTA ATGCCTCCTT CACTGGGCCT TCCTAGCAGG 3701 ATAGAAAGTC CTTGCCCAGA GCAGGACCTG GCTGTCTTTT TTTTTTTTTT 3751 TTTCCCGAGA CCAAGTTTCA CTCTGTTGCC CAAGGTAGAG TGCAGTGGCG 3801 TGATCTCTGC TCATTGCAAC TGCCGCCTCC CGGGTTCAAG CAATTCTCAT 3851 GCATCAGCCT CCCAAGTACC TGGGACTACA GGCGTGAGCT ACCATGCCCG 3901 GCTAATTTTT GTATTTTTAG TAGAGATGGG GTTTCATTAT GTTGGCCAGG 3951 CTGGTCTCGA ACTCCTTACC TCAGGTGATC CACCCACCTT GGCCTCCCGA 4001 AGTGCTGGGA TTACAGGCAT GAGCCACTGC GCCCGGCCAT GGACCTGGCT 4051 GTCTTTATCA TCCCCACAAA CATTTTGAAA CTGGAATATT TGTCTTCAGA 4101 AAATGGAAAC AAGACTATAA ATGATAAGCC CTGTCCCTAG CACCACCTCT 4151 CCTGTGTGTG GAATAGAGGC CCCTCGTGCT ACCAACACTT ACCCTGTGTT 4201 TAAAAAGATC TTGTACCAAG CCAACGGCGT TCCTGGCTCT CCTGCCCACA 4251 GGATGAACAT TTTCGGCTTC CTTAGGAGTT TTGCCCTACC GTATTCCAAA 4301 GCGTGTGCTG GTTTCTCATA TTGTCTGTAG GCTCACTCAG CCCGCAGTTT 4351 ATGTGTGTGC TTTTTTCTAT GAAAAATGAT GTATTTTGCT ACTTCCTGTG 4401 TACAAAGTTT TATTGTAAAT GTTTTTTGTG CTTTGCATGA ACAGGGGCCA 4451 CGTTGTTGCA ATTGTTTCAG TAGAACTGGT TTGATTTCTA AAATGTTCCT 4501 GTAACATATC TTTTATGAAC AAATCTGAAC AATTTGTGAA ATAAAACATT 4551 GAAAACCAAA AAAAAAAAAA AAAA BLAST Results Entry HS834352 from database EMBL : human STS WI -15502 . Score = 1331 , P = 5 . 4e- 54 , identities = 287 /301 Medline entries 98146272: cDNA cloning of a novel trypsin inhibitor with similarity to pathogenesis-related proteins, and its frequent expression in human brain cancer cells. Peptide information for frame 1 ORF from 205 bp to 1695 bp; peptide length: 497 Category: strong similarity to known protein 1 MSCVLGGVIP LGLLFLVCGS QGYLLPNVTL LEELLSKYQH NESHSRVRRA 51 IPREDKEEIL MLHNKLRGQV QPQASNMEYM TWDDELEKSA AAWASQCIWE 101 HGPTSLLVSI GQNLGAHWGR YRSPGFHVQS WYDEVKDYTY PYPSECNPWC 151 PERCSGPMCT HYTQIVWATT NKIGCAVNTC RKMTVWGEVW ENAVYFVCNY 201 SPKGNWIGEA PYKNGRPCSE CPPSYGGSCR NNLCYREETY TPKPETDEMN 251 EVETAPIPEE NHVWLQPRVM RPTKPKKTSA VNYMTQVVRC DTKMKDRCKG 301 STCNRYQCPA GCLNHKAKIF GTLFYESSSS ICRAAIHYGI LDDKGGLVDI 351 TRNGKVPFFV KSERHGVQSL SKYKPSSSFM VSKVKVQDLD CYTTVAQLCP 401 FEKPATHCPR IHCPAHCKDE PSYWAPVFGT NIYADTSSIC KTAVHAGVIS 451 NESGGDVDVM PVDKKKTYVG SLRNGVQSES LGTPRDGKAF RIFAVRQ BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_4b4, frame 1 TREMBLNEW:AF109674_1 gene: "Lgll"; product: "late gestation lung protein 1"; Rattus norvegicus late gestation lung protein 1 (Lgll) mRNA, complete eds., N = 1, Score = 968, P = 1.9e-97 TREMBL :D45027_1 product: "25 kDa trypsin inhibitor"; Homo sapiens mRNA for 25 kDa trypsin inhibitor, complete eds., N = 1, Score = 738, P = 4.5e-73 TREMBL :AB009609_1 gene: "HrTT-1"; Halocynthia roretzi HrTT-1 mRNA, complete eds., N = 1, Score = 345, P = 2e-31 PIR:JC5308 testis-specific, vespid, and pathogenesis-related protein 1 precursor - human, N = 1, Score = 337, P = 1.7e-30 >TREMBLNEW:AF109674_1 gene: "Lgll"; product: "late gestation lung protein 1"; Rattus norvegicus late gestation lung protein 1 (Lgll) mRNA, complete eds . Length = 188 HSPs: Score = 968 (145.2 bits), Expect = 1.9e-97, P = 1.9e-97 Identities = 160/185 (86%), Positives = 170/185 (91%) Query: 61 MLHNKLRGQVQPQASNMEYMTWDDELEKSAAAWASQCIWEHGPTSLLVSIGQNLGAHWGR 120 MLHNKLRGQV P ASNMEYMTWD+ELE+SAAAWA +C+WEHGP SLLVSIGQNL HWGR Sbjct: 1 MLHNKLRGQVYPPASNMEYMTWDEELERSAAAWAQRCLWEHGPASLLVSIGQNLAVHWGR 60 Query: 121 YRSPGFHVQSWYDEVKDYTYPYPSECNPWCPERCSGPMCTHYTQIVWATTNKIGCAVNTC 180 YRSPGFHVQSWYDEVKDYTYPYP ECNPWCPERCSG MCTHYTQ+VWATTNKIGCAV+TC Sbjct: 61 YRSPGFHVQSWYDEVKDYTYPYPHECNPWCPERCSGAMCTHYTQMVWATTNKIGCAVHTC 120 Query: 181 RKMTVWGEVWENAVYFVCNYSPKGNWIGEAPYKNGRPCSECPPSYGGSCRNNLCYREETY 240 R M+VWG++WENAVY VCNYSPKGNWIGEAPYK+GRPCSECP SYGG CRNNLCYREE Y Sbjct: 121 RSMSVWGDIWENAVYLVCNYSPKGNWIGEAPYKHGRPCSECPSSYGGGCRNNLCYREEHY 180 Query: 241 TPKPE 245 KPE Sbjct: 181 HQKPE 185 Pedant information for DKFZphtes3_4b4, frame 1 Report for DKFZphtes3_4b4.1 [LENGTH] 497 [MW] 55920.00 [pi] 8.36 [HOMOL] TREMBL :D45027_1 product: "25 kDa trypsin inhibitor"; Homo sapiens mRNA for 25 kDa trypsin inhibitor, complete eds. 6e-78 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YJL078c] 8e-12 [BLOCKS] BL01009E Extracellular proteins ΞCP/Tpx-l/Ag5/PR-l/Sc7 proteins [BLOCKS] BL01009D Extracellular proteins SCP/Tpx-l/Ag5/PR-l/Sc7 proteins [BLOCKS] BL01009C Extracellular proteins SCP/Tpx-l/Ag5/PR-l/Sc7 proteins [BLOCKS] BL01009A Extracellular proteins SCP/Tpx-l/Ag5/PR-l/Sc7 proteins [PIRKW] glycoprotein 5e-22 [PIRKW] blocked ammo end 5e-13 [PIRKW] brain 9e-30 [PIRKW] hydrolase 4e-09 [PIRKW] hemolymph coagulation 4e-09 [PIRKW] zymogen 4e-09 [PIRKW] alternative splicing 4e-09 [PIRKW] sperm 5e- 2 2 [PIRKW] viroid-induced protein 2e-ll [PIRKW] venom 6e-18 [PIRKW] pyroglutamic acid 2e-ll [PIRKW] transmembrane protein 2e-10 [PIRKW] serine proteinase 4e-09 [SUPFAM] C-type lectin homology 4e-09 [SUPFAM] trypsin homology 4e-09 [SUPFAM] complement factor H repeat homology 4 e-09 [SUPFAM] cyste e-rich secretory protein 1 6e-24 [SUPFAM] pathogenesis -related leaf pr otein 7e-15 [PROSITE] MYRISTYL 8 [PROSITE] CAMP_PHOSPHO_SITE 3 [PROSITE] CK2_PHOSPHO_SITE 6 [PROSITE] TYR_PHOSPHO_SITE 1 [PROSITE] PKC_PHOSPHO_SITE 8 [PROSITE] ASN_GLYCOSYLATION 3 [PROSITE] SCP_AG5_PR1_SC7_2 1 [PFAM] SCP-li ke extracellular Proteins [KW] All_Beta [KW] SIGNAL_PEPTIDE 23 [KW] LOW COMPLEXITY 1.21 % SEQ MΞCVLGGVIPLGLLFLVCGSQGYLLPNVTLLEELLSKYQHNESHSRVRRAIPREDKEEIL SEG xxxxxx PRD ccceeeeeceeeeeeeecccccccccchhhhhhhhhhhhhcccchhhhhhhccchhhhhh SEQ MLHNKLRGQVQPQASNMEYMTWDDELEKSAAAWASQCIWEHGPTSLLVSIGQNLGAHWGR SEG PRD hhhhhhhcccccccccchhhhhhhhhhhhhhhhhhhhcccccccccccccccceeeeecc SEQ YRSPGFHVQSWYDEVKDYTYPYPSECNPWCPERCSGPMCTHYTQIVWATTNKIGCAVNTC SEG PRD ccccchhhhhhhhhhhccccccccccccccccccccccccceeeeeeeccccccceeeec SEQ RKMTVWGEVWENAVYFVCNYSPKGNWIGEAPYKNGRPCSECPPSYGGSCRNNLCYREETY SEG PRD cccccccccccceeeeeeeccccccccccccccccccccccccccccccccccccccccc SEQ TPKPETDEMNEVETAPIPEENHVWLQPRVMRPTKPKKTSAVNYMTQVVRCDTKMKDRCKG SEG PRD cccccccccccccccccccceeeeecccccccccccceeeeeeeeeeeeecccccccccc SEQ STCNRYQCPAGCLNHKAKIFGTLFYESSSSICRAAIHYGILDDKGGLVDITRNGKVPFFV SEG PRD ccccccccccccccccceeeeeeeeecccceeeeeccccccccccceeeeeccccceeee SEQ KSERHGVQSLSKYKPSSSFMVSKVKVQDLDCYTTVAQLCPFEKPATHCPRIHCPAHCKDE SEG PRD eccceeeeeeeeccccceeeeeeeeeecccceeeeeeeeccccccccccccccccccccc SEQ PSYWAPVFGTNIYADTSSICKTAVHAGVISNESGGDVDVMPVDKKKTYVGSLRNGVQSES SEG PRD ccceeeeeceeeccccceeeeeeeeccccccccccccceeecccceeeeeecccceeeee SEQ LGTPRDGKAFRIFAVRQ SEG PRD ccccccccceeeeeccc Prosite for DKFZphtes3_4b4.1 PS00001 27->31 ASN_GLYCOSYLATION PDOC00001 PS00001 41->45 ASN_GLYCOSYLATION PDOC00001 PS00001 451->455 ASN_GLYCOSYLATION PDOC00001 PS00004 181->185 CAMP_PHOSPHO_SITE PDOC00004 PS00004 276->280 CAMP_PHOSPHO_SITE PDOC00004 PS00004 464->468 CAMP_PHOSPHO_SITE PDOC00004 PS00005 170->173 PKC_PHOSPHO_SITE PDOC00005 PS00005 179->182 PKC_PHOSPHO_SITE PDOC00005 PS00005 201->204 PKC_PHOSPHO_SITE PDOC00005 PS00005 228->231 PKC_PHOSPHO_SITE PDOC00005 PS00005 241->244 PKC_PHOSPHO_SITE PDOC00005 PS00005 362->365 PKC_PHOSPHO_SITE PDOC00005 PS00005 471->474 PKC_PHOSPHO_SITE PDOC00005 PS00005 483->486 PKC_PHOSPHO_SITE PDOC00005 PS00006 29->33 CK2_PHOSPHO_SITE PDOC00006 PS00006 75->79 CK2_PHOSPHO_SITE PDOC00006 PS00006 81->85 CK2_PHOSPHO_SITE PDOC00006 PS00006 130->134 CK2_PHOSPHO_SITE PDOC00006 PS00006 453->457 CK2_PHOSPHO_SITE PDOC00006 PS00006 483->487 CK2_PHOSPHO_SITE PDOC00006 PS00007 385->393 TYR_PHOSPHO_SITE PDOC00007 PS00008 111->117 MYRISTYL PDOC00008 PS00008 115->121 MYRISTYL PDOC00008 PS00008 174->180 MYRISTYL PDOC00008 PS00008 204->210 MYRISTYL PDOC00008 PS00008 227->233 MYRISTYL PDOC00008 PS00008 300->306 MYRISTYL PDOC00008 PS00008 447->453 MYRISTYL PDOC00008 PS00008 470->476 MYRISTYL PDOC00008 PS01010 195->207 SCP AG5 PR1 SC7 2 PDOC00772 Pfam for DKFZphtes3_4b4.1 HMM_NAME SCP-like extracellular Proteins HMM *PQDEQDEWLNkHNDFRQQVGRGLETRGNPGPQPPAsNMnPMVWNDELAt P + ++E+L HN +R QV P ASNM M+W+DEL + Query 52 PREDKEEILMLHNKLRGQVQ PQASNMEYMTWDDELEK 88 HMM IAQnWANQCiFDHHDCCWNHsnYPYGQNIAWWSsTANnPWnWssMIQMWY A WA+QCI +H ++ + S GQN+ + + ++++ +Q+WY Query 89 SAAAWASQCIWEHGPTSLLVSI GQNLGAHWG RYRSPGFHVQΞWY 132 HMM NEvkDYNYNWNTCkGG NNFmVCGHYTQMVWRnTfrIGCGRYICYC +EVKDY Y + + +C HYTQ+VW+ T +IGC+ C+ Query 133 DEVKDYTYPYPSECNPWCPERCSGPMCTHYTQIVWATTNKIGCAVNTCRK 182 HMM NNNWrKPDPWKhkWYYVCNYCPpGNYmN* + W + W+ +Y VCNY P+GN+++ Query 183 MTV —GEVWENAVYFVCNYSPKGNWIG 208 DKFZphtes3_4fl7 group: testes derived DKFZphtes3_4f17 encodes a novel 656 amino acid protein with weak similarity to methyl-CpG- bindmg proteins. Methylation at the DNA sequence 5 ' -CpG is required for mammalian development. Methyl-CpG- binding proteins bind specifically to methylated DNA via a related ammo acid motif and can repress transcription. The novel protein does not contain such a motife. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . similarity to methyl-CpG-bindmg protein extension of HS557771/HSZ78337, there are some differences to these sequences Sequenced by AGOWA Locus: /map="18" Insert length : 2320 bp Poly A stretch at pos . 2266 , polyadenylation signal at pos . 2251 1 GGCAGGTTCG CGGGTCGCTG GCGGGGGTCG TGAGGGAGTG CGCCGGGAGC 51 GGAGATATGG AGGGAGATGG TTCAGACCCA GAGCCTCCAG ATGCCGGGGA 101 GGACAGCAAG TCCGAGAATG GGGAGAATGC GCCCATCTAC TGCATCTGCC 151 GCAAACCGGA CATCAACTGC TTCATGATCG GGTGTGACAA CTGCAATGAG 201 TGGTTCCATG GGGACTGCAT CCGGATCACT GAGAAGATGG CCAAGGCCAT 251 CCGGGAGTGG TACTGTCGGG AGTGCAGAGA GAAAGACCCC AAGCTAGAGA 301 TTCGCTATCG GCACAAGAAG TCACGGGAGC GGGATGGCAA TGAGCGGGAC 351 AGCAGTGAGC CCCGGGATGA GGGTGGAGGG CGCAAGAGGC CTGTCCCTGA 401 TCCAGACCTG CAGCGCCGGG CAGGGTCAGG GACAGGGGTT GGGGCCATGC 451 TTGCTCGGGG CTCTGCTTCG CCCCACAAAT CCTCTCCGCA GCCCTTGGTG 501 GCCACACCCA GCCAGCATCA CCAGCAGCAG CAGCAGCAGA TCAAACGGTC 551 AGCCCGCATG TGTGGTGAGT GTGAGGCATG TCGGCGCACT GAGGACTGTG 601 GTCACTGTGA TTTCTGTCGG GACATGAAGA AGTTCGGGGG CCCCAACAAG 651 ATCCGGCAGA AGTGCCGGCT GCGCCAGTGC CAGCTGCGGG CCCGGGAATC 701 GTACAAGTAC TTCCCTTCCT CGCTCTCACC AGTGACGCCC TCAGAGTCCC 751 TGCCAAGGCC CCGCCGGCCA CTGCCCACCC AACAGCAGCC ACAGCCATCA 801 CAGAAGTTAG GGCGCATCCG TGAAGATGAG GGGGCAGTGG CGTCATCAAC 851 AGTCAAGGAG CCTCCTGAGG CTACAGCCAC ACCTGAGCCA CTCTCAGATG 901 AGGACCTACC TCTGGATCCT GACCTGTATC AGGACTTCTG TGCAGGGGCC 951 TTTGATGACC ATGGCCTGCC CTGGATGAGC GACACAGAAG AGTCCCCATT 1001 CCTGGACCCC GCGCTGCGGA AGAGGGCAGT GAAAGTGAAG CATGTGAAGC 1051 GTCGGGAGAA GAAGTCTGAG AAGAAGAAGG AGGAGCGATA CAAGCGGCAT 1101 CGGCAGAAGC AGAAGCACAA GGATAAATGG AAACACCCAG AGAGGGCTGA 1151 TGCCAAGGAC CCTGCGTCAC TGCCCCAGTG CCTGGGGCCC GGCTGTGTGC 1201 GCCCCGCCCA GCCCAGCTCC AAGTATTGCT CAGATGACTG TGGCATGAAG 1251 CTGGCAGCCA ACCGCATCTA CGAGATCCTC CCCCAGCGCA TCCAGCAGTG 1301 GCAGCAGAGC CCTTGCATTG CTGAAGAGCA CGGCAAGAAG CTGCTCGAAC 1351 GCATTCGCCG AGAGCAGCAG AGTGCCCGCA CCCGCCTTCA GGAAATGGAA 1401 CGCCGATTCC ATGAGCTTGA GGCCATCATT CTACGTGCCA AGCAGCAGGC 1451 TGTGCGCGAG GATGAGGAGA GCAACGAGGG TGACAGTGAT GACACAGACC 1501 TGCAGATCTT CTGTGTTTCC TGTGGGCACC CCATCAACCC ACGTGTTGCC 1551 TTGCGCCACA TGGAGCGCTG CTACGCCAAG TATGAGAGCC AGACGTCCTT 1601 TGGGTCCATG TACCCCACAC GCATTGAAGG GGCCACACGA CTCTTCTGTG 1651 ATGTGTATAA TCCTCAGAGC AAAACATACT GTAAGCGGCT CCAGGTGCTG 1701 TGCCCCGAGC ACTCACGGGA CCCCAAAGTG CCAGCTGACG AGGTATGCGG 1751 GTGCCCCCTT GTACGTGATG TCTTTGAGCT CACGGGTGAC TTCTGCCGCC 1801 TGCCCAAGCG CCAGTGCAAT CGCCATTACT GCTGGGAGAA GCTGCGGCGT 1851 GCGGAAGTGG ACTTGGAGCG CGTGCGTGTG TGGTACAAGC TGGACGAGCT 1901 GTTTGAGCAG GAGCGCAATG TGCGCACAGC CATGACAAAC CGCGCGGGAT 1951 TGCTGGCCCT GATGCTGCAC CAGACGATCC AGCACGATCC CCTCACTACC 2001 GACCTGCGCT CCAGTGCCGA CCGCTGAGCC TCCTGGCCCG GACCCCTTAC 2051 ACCCTGCATT CCAGATGGGG GAGCCGCCCG GTGCCCGTGT GTCCGTTCCT 2101 CCACTCATCT GTTTCTCCGG TTCTCCCTGT GCCCATCCAC CGGTTGACCG 2151 CCCATCTGCC TTTATCAGAG GGACTGTCCC CGTCGACATG TTCAGTGCCT 2201 GGTGGGGCTG CGGAGTCCAC TCATCCTTGC CTCCTCTCCC TGGGTTTTGT 2251 TAATAAAATT TTGAAGAAAC CAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 2301 AAAAAAAAAA AAAAAAAAAA BLAST Results Entry HS557771 from database EMBLEST : Human chromosome 18 clone 2 mRNA sequence. Score = 7582, P = 0.0e+00, identities = 1560/1598 Entry HSZ78337 from database EMBLEST: H. sapiens mRNA, expressed sequence tag ICRFp507H02194 (5') Score = 6339, P = 9.0e-281, identities = 1307/1347 Entry HS095149 from database EMBL: human STS WI-6941. Score = 1210, P = 2.2e-49, identities = 246/251 Medline entries 98449942: Identification and characterization of a family of mammalian methyl-CpG binding proteins . 9824997: Gene silencing by methyl-CpG-bmding proteins. Peptide information for frame 3 ORF from 57 bp to 2024 bp; peptide length: 656 Category: similarity to known protein 1 MEGDGSDPEP PDAGEDSKSE NGENAPIYCI CRKPDINCFM IGCDNCNEWF 51 HGDCIRITEK MAKAIREWYC RECREKDPKL EIRYRHKKSR ERDGNERDSS 101 EPRDEGGGRK RPVPDPDLQR RAGSGTGVGA MLARGSASPH KSSPQPLVAT 151 PSQHHQQQQQ QIKRSARMCG ECEACRRTED CGHCDFCRDM KKFGGPNKIR 201 QKCRLRQCQL RARESYKYFP SSLSPVTPSE SLPRPRRPLP TQQQPQPSQK 251 LGRIREDEGA VASSTVKEPP EATATPEPLS DEDLPLDPDL YQDFCAGAFD 301 DHGLPWMSDT EESPFLDPAL RKRAVKVKHV KRREKKSEKK KEERYKRHRQ 351 KQKHKDKWKH PERADAKDPA SLPQCLGPGC VRPAQPSSKY CSDDCGMKLA 401 ANRIYEILPQ RIQQWQQSPC IAEEHGKKLL ERIRREQQSA RTRLQEMERR 451 FHELEAIILR AKQQAVREDE ESNEGDSDDT DLQIFCVSCG HPINPRVALR 501 HMERCYAKYE ΞQTSFGSMYP TRIEGATRLF CDVYNPQSKT YCKRLQVLCP 551 EHSRDPKVPA DEVCGCPLVR DVFELTGDFC RLPKRQCNRH YCWEKLRRAE 601 VDLERVRVWY KLDELFEQER NVRTAMTNRA GLLALMLHQT IQHDPLTTDL 651 RSSADR BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_4f17, frame 3 TREMBL :CEF52B11_4 gene: "F52B11.1"; Caenorhabditis elegans cosmid F52B11, N = 2, Score = 316, P = 8.8e-27 TREMBL:HSAB2331_1 gene: "KIAA0333"; Human mRNA for KIAA0333 gene, partial eds., N = 2, Score = 163, P = 2.8e-13 TREMBL :SPCC594_5 gene: "SPCC594.05c"; product: "putative transcriptional regulatory protein, phd finger containing"; S. pombe chromosome III cosmid c594. , N = 3, Score = 168, P = 3.6e-12 TREMBL:AF072240_1 gene: "Mbdl"; product: "methyl-CpG binding protein MBDl"; Mus musculus methyl-CpG binding protein MBD1 (Mbdl) mRNA, complete eds., N = 2, Score = 189, P = 7.6e-ll >TREMBL : CEF52B11_4 gene : • F52B11 . 1 ' Caenorhabditis elegans cosmid F52B11 Length = 523 HSPs : Score = 316 (47.4 bits), Expect = 8.8e-27, Sum P(2) = 3e-27 Identities = 100/336 (29%), Positives = 167/336 (49%) Query: 333 REKKSEKKKEERYKRHRQ-KQKHKDKWKHPERADAKDPASLP-QCLGPGCVRPAQPSSKY 390 +++K+ E Y +R +Q+ D + + +A +P P QCL P C+ ++ SKY Sbjct: 118 QQRKANIINERDYVPNRPTRQQSADLRRKRTQLNA-EPDKHPRQCLNPNCIYESRIDSKY 176 Query: 391 CSDDCGMKLAANRIYEILPQRIQQW QQSPCIAEEHGKKLLERIRREQQSARTRLQ 445 CSD+CG +LA R+ EILP R +Q+ P E+ K +1 RE Q + Sbjct: 177 CSDECGKELARMRLTEILPNRCKQYFFEGPSGGPRSLEDEIKPKRAKINREVQKLTESEK 236 Query: 446 EMERRFHEL-EAIILRAKQQAVREDEESNEGDSDDTDLQIFCVSCGHPINPRVAL-RHME 503 M ++L E I + K Q + +E D +L C+ CG P P + +H+E Sbjct: 237 NMMAFLNKLVEFIKTQLKLQPLGTEERY DDNLYEGCIVCGLPDIPLLKYTKHIE 290 Query: 504 RCYAKYESQTSFGSMYPTRIEGATRLFCDVYNPQSKTYCKRLQVLCPEHSRDPKVPADEV 563 C+A+ E SFG+ P + +C+ Y+ ++ ++CKRL+ LCPEH + +V Sbjct: 291 LCWARSEKAISFGA—PEK--NNDMFYCEKYDSRTNSFCKRLKSLCPEHRKLGDEQHLKV 346 Query: 564 CGCP LVRDVFELTGDF CRLPKRQCNRHYCWEKLRRAEVDLERVR 607 CG P V ++ E+ F CR K C++H+ W R ++LE+ Sbjct: 347 CGYPKKWEDGMIETAKTVSELIEMEDPFGEEGCRTKKDACHKHHKWIPSLRGTIELEQAC 406 Query: 608 VWYKLDELFEQ--ERNVRTAMTNRAGLLALMLHQTIQHDPLTTDLRSΞA 654 ++ K+ EL + + N T A L++M+H+ + + LR+ A Sbjct: 407 LFQKMYELCHEMHKLNAHAEWTTNA—LΞIMMHKQPSTEKCSFFLRNFA 453 Score = 53 (8.0 bits), Expect = 8.8e-27, Sum P(2) = 3e-27 Identities = 24/100 (24%), Positives = 41/100 (41%) Query: 169 CGECEACRRTEDCGHCDFCR DMKK-FGGPNKIRQKCRLRQCQLRARESYKYFPSS 222 C C C ++CG C CR DM+K F +K + RQ + + + Sbjct: 17 CMNCIRCNDEKNCGTCWPCRNGKTCDMRKCFSAKRLYNEKVK-RQTDENLK-AIMAKTAQ 74 Query: 223 LSPVTPSESLPRPRRPLPTQQQPQPSQKLGRIR-EDEGAVASS 264 + + P P+ +QQ + +K GR + G A++ Sbjct: 75 REAAHQAATTTAPSAPVVIEQQVE-KKKRGRKKGSGNGGAAAA 116 Score = 48 (7.2 bits), Expect = 2.9e-26, Sum P(2) = 2.9e-26 Identities = 13/39 (33%), Positives = 19/39 (48%) Query: 179 EDCGHCDFCRDMKKFGG—PNKIRQKCRLRQCQLRARESY 216 E C +C C D K G P + + C +R+C A+ Y Sbjct: 15 ERCMNCIRCNDEKNCGTCWPCRNGKTCDMRKC-FSAKRLY 53 Pedant information for DKFZphtes3_4f17, frame 3 Report for DKFZphtes3_4f17.3 [LENGTH] 656 [MW] 75711.71 [pi] 8.61 [HOMOL] TREMBL :CEF52B11_4 gene: "F52B11.1"; Caenorhabditis elegans cosmid F52B11 3e-25 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YPL138c] 3e-10 [FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YNL097c] 2e-04 [PROSITE] MYRISTYL 6 [PROSITE] AMIDATION 2 [PROSITE] CK2 PHOSPHO SITE 8 [PROSITE] TYR PHOSPHO SITE 3 [PROSITE] GLYCOSAMINOGLYCAN 1 [PROSITE] PKC PHOSPHO SITE 9 [KW] All Alpha [KW] LOW COMPLEXITY 18.75 % [KW] COILED COIL 4.57 % SEQ MEGDGSDPEPPDAGEDSKSENGENAPIYCICRKPDINCFMIGCDNCNEWFHGDCIRITEK SEG PRD cccccccccccccccccccccccccceeeeeeccccceeeeecccccccccccchhhhhh COILS SEQ MAKAIREWYCRECREKDPKLEIRYRHKKSRERDGNERDSSEPRDEGGGRKRPVPDPDLQR SEG PRD hhhhhhhhhhhccccccccchhhhhhhhhccccccccccccccccccccccccccccccc COILS SEQ RAGSGTGVGAMLARGSASPHKSSPQPLVATPSQHHQQQQQQIKRSARMCGECEACRRTED SEG xxxxxxxxx PRD cccccccceeeecccccccccccccccccchhhhhhhhhhhhhhhhhhcccccccccccc COILS SEQ CGHCDFCRDMKKFGGPNKIRQKCRLRQCQLRARESYKYFPSSLSPVTPSESLPRPRRPLP SEG xxxxxxxxxxxxxx xxxxxxxxxxxxxx PRD cccccccccccccccccchhhhhhhhhhhhhhhhhhcccccccccccccccccccccccc COILS SEQ TQQQPQPSQKLGRIREDEGAVAΞSTVKEPPEATATPEPLSDEDLPLDPDLYQDFCAGAFD SEG xxxxxxxxx xxxxxxxxxxxxxxxxxxxxxxx PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc COILS SEQ DHGLPWMSDTEESPFLDPALRKRAVKVKHVKRREKKSEKKKEERYKRHRQKQKHKDKWKH SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD cccccccccccccccccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccchh COILS SEQ PERADAKDPASLPQCLGPGCVRPAQPSSKYCSDDCGMKLAANRIYEILPQRIQQWQQSPC SEG PRD hhhhhccccccccccccccccccccccccccccccchhhhhhhhhhhhhhhhhhhhccch COILS SEQ IAEEHGKKLLERIRREQQSARTRLQEMERRFHELEAIILRAKQQAVREDEESNEGDSDDT SEG xxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccccccccc COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCC SEQ DLQIFCVΞCGHPINPRVALRHMERCYAKYESQTSFGSMYPTRIEGATRLFCDVYNPQSKT SEG x PRD ceeeeeeeccccccccchhhhhhhhhhhhhhcccccccccccccccceeeeeeccccccc COILS SEQ YCKRLQVLCPEHSRDPKVPADEVCGCPLVRDVFELTGDFCRLPKRQCNRHYCWEKLRRAE SEG PRD cchhhhhhhccccccccccceeeeccccchhhhhccccccccccccccchhhhhhhhhhh COILS SEQ VDLERVRVWYKLDELFEQERNVRTAMTNRAGLLALMLHQTIQHDPLTTDLRSSADR SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccccccccccc COILS Prosite for DKFZphtes3_4f17.3 PS00002 124-M28 GLYCOSAMINOGLYCAN PDOC00002 PS00005 58->61 PKC_PHOSPHO_SITE PDOC00005 PS00005 165->168 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 215->218 PKC_PHOΞPHO_SITE PDOC00005 PS00005 248->251 PKC_PHOSPHO_SITE PDOC00005 PS00005 265->268 PKC_PHOSPHO_SITE PDOC00005 PS00005 337->340 PKC_PHOSPHO_SITE PDOC00005 PS00005 387->390 PKC_PHOSPHO_SITE PDOC00005 PS00005 439->442 PKC_PHOSPHO_SITE PDOC00005 PS00005 627->630 PKC_PHOSPHO_SITE PDOC00005 PS00006 6->10 CK2_PHOSPHO_SITE PDOC00006 PS00006 17->21 CK2_PHOSPHO_SITE PDOC00006 PS00006 227->231 CK2_PHOSPHO_ΞITE PDOC00006 PS00006 265->269 CK2_PHOSPHO_SITE PDOC00006 PS00006 280->284 CK2_PHOSPHO_SITE PDOC00006 PS00006 308->312 CK2_PHOSPHO_SITE PDOC00006 PS00006 521->525 CK2_PHOSPHO_SITE PDOC00006 PS00006 652->656 CK2_PHOSPHO_SITE PDOC00006 PS00007 339->346 TYR_PHOSPHO_SITE PDOC00007 PS00007 500->507 TYR_PHOSPHO_SITE PDOC00007 PS00007 211->219 TYR_PHOSPHO_SITE PDOC00007 PS00008 42->48 MYRISTYL PDOC00008 PS00008 123->129 MYRISTYL PDOC00008 PS00008 125->131 MYRISTYL PDOC00008 PS00008 129->135 MYRISTYL PDOC00008 PS00008 259->265 MYRISTYL PDOC00008 PS00008 396->402 MYRISTYL PDOC00008 PS00009 107->111 AMIDATION PDOC00009 PS00009 425->429 AMIDATION PDOC00009 (No Pfam data available for DKFZphtes3 4fl7.3) DKFZphtes3_4f5 group: signal transduction DKFZphtes3 4f5.3encodes a novel 790 amino acid protein similar to beta-transducins . The protein contains 3 WD-40 repeats, which are typical for the beta-transducm subunit of G- proteins. The beta subunits seem to be required for the replacement of GDP by GTP as well as for membrane anchoring and receptor recognition. In addition, a Cytochrome C family heme- binding site signature is present. The protein is larger (790 ammo acids (than the usual eukaryotic G-beta transduc s (about 340 amino acids) . The new protein can find application in modulating/blocking G-protem-dependent pathways. similarity to S. pombe "beta-transducm" complete cDNA, EST hits complete eds, on genomic level encoded by HS313D11, at least 7 exons these exons match only partialy with the predicted transcripts in HS313D11 Sequenced by AGOWA Locus: /map="16pl3.3" Insert length: 3166 bp No poly A stretch found, no polyadenylation signal found 1 GGCGGCTTCC GGCGCGGCGG TTCCGGACAA CCGTGCGCTT TTAGTAAAAG 51 ATTGGGGTTC GCGCGGGGGA GAAGGGCTGC CCCGGGCCCT CTGGTTCTCG 101 TCCCGCAGCG TCCGCTCCCC CGCGCCACTG CGCCGCTCCC AGGAACCCTG 151 TACTCCGGGG TCGCCGGCTT CTCTCCTGCC TCCGGTCCCG CCAGACACCT 201 CGAGCTCCTT AAGTAGCTCG GTCCTTGACG TCCCTCTGGG CCCTTCCCGC 251 GTCTATCGCC TGAGTCCCCG GGCCCCTCTA GCCCTCTGTT CCCTCCCCTC 301 TTTTGTTCCT CCCTAGAGCC CCGCCGCCCT CAGGGCTGAC AGTGTGGACG 351 GCGGGAGTCT CCTCGCTCCC CTGCTGGGAT TGACTGACCG AGCGTTTAGT 401 GACTGCCCAG ATCTGGCTGA TGGGGGTACC GAGAGGTGGC CTGGGCCGGG 451 AATGTCCAGC TAGAGTCTTC CGTGGAAGTC AGACATGAAA CTGACAGGCC 501 TAAGGGAAGC TAGGAAGTCC CCTCACCGCT CAGCCAGGGT GATGGGCTGG 551 ACTGACAGAC TCCAGTGAAT TTGAGCTTGC CTGTCAGGCT GATTGGCTGA 601 TAGACAGCCC TGGATTGGCT CACTAAGACT GACCAGCCCG GGACCAAGCA 651 GTTCTGGGGT CCCAACCTGG GTGGAAGGTC TGAACTGATG ACCCACCCAG 701 GCTGACCAGG CCAGCCCACC TCACTGACCT CCTGACCCCT GACCTCATCA 751 CCTGTGCAGC CATGGAGAAG ATGTCCCGTG TGACCACAGC CCTGGGTGGC 801 AGCGTGCTGA CAGGCCGCAC CATGCACTGC CACCTGGATG CTCCCGCCAA 851 TGCCATCAGT GTGTGCCGCG ACGCAGCCCA GGTGGTCGTG GCAGGCCGTA 901 GCATCTTCAA GATCTATGCC ATCGAGGAGG AACAGTTCGT GGAAAAGCTG 951 AACCTGCGTG TGGGGCGCAA GCCTTCGCTT AACCTGAGCT GTGCTGACGT 1001 GGTCTGGCAC CAGATGGATG AGAACCTGCT GGCCACAGCA GCCACCAATG 1051 GCGTGGTGGT CACGTGGAAC CTGGGCCGGC CATCCCGCAA CAAGCAGGAC 1101 CAGCTGTTCA CAGAACACAA GCGCACGGTA AACAAAGTCT GCTTCCACCC 1151 CACCGAAGCC CACGTGCTGC TCAGTGGCTC CCAGGATGGC TTCATGAAGT 1201 GCTTTGACCT CCGCAGAAAG GACTCTGTCA GCACCTTCTC GGGCCAGTCG 1251 GAGAGCGTGC GGGACGTGCA GTTCAGTATC CGGGACTACT TCACCTTCGC 1301 CTCCACCTTT GAGAACGGCA ATGTGCAGCT CTGGGACATC CGGCGTCCCG 1351 ACCGGTGCGA GAGGATGTTC ACAGCCCACA ACGGACCCGT CTTCTGCTGC 1401 GACTGGCACC CCGAGGACAG GGGCTGGTTG GCCACTGGAG GGCGCGACAA 1451 GATGGTGAAG GTCTGGGACA TGACCACGCA CCGTGCCAAG GAGATGCACT 1501 GTGTGCAGAC CATCGCCTCG GTGGCCCGTG TGAAGTGGCG GCCAGAGTGC 1551 CGCCACCACC TGGCCACGTG CTCCATGATG GTGGACCACA ACATCTATGT 1601 TTGGGACGTG CGCCGGCCCT TCGTGCCAGC TGCCATGTTT GAGGAACACC 1651 GAGACGTCAC CACGGGAATT GCCTGGCGCC ACCCCCACGA CCCCTCCTTC 1701 CTGCTGTCTG GCTCCAAGGA CAGCTCGCTG TGCCAGCACC TGTTCCGCGA 1751 CGCCAGCCAG CCCGTCGAGC GCGCCAACCC TGAGGGCCTC TGCTACGGCC 1801 TCTTCGGGGA CCTGGCCTTC GCCGCCAAGG AGAGCCTCGT GGCTGCCGAG 1851 TCGGGGCGCA AGCCCTACAC TGGCGACCGG CGCCACCCCA TCTTCTTTAA 1901 GCGCAAGCTG GACCCTGCCG AGCCCTTCGC AGGCCTCGCC TCCAGTGCCC 1951 TCAGTGTCTT TGAGACGGAG CCAGGTGGCG GCGGCATGCG CTGGTTTGTG 2001 GACACAGCTG AGCGTTATGC GCTGGCTGGC CGGCCACTGG CCGAGCTCTG 2051 TGACCACAAC GCAAAGGTGG CTCGAGAGCT TGGCCGCAAC CAGGTGGCGC 2101 AAACGTGGAC CATGCTGCGG ATCATCTACT GCAGCCCTGG CCTAGTGCCC 2151 ACTGCAAACC TCAACCACAG TGTGGGCAAG GGTGGCTCCT GTGGCCTCCC 2201 GCTCATGAAC AGTTTCAACC TGAAGGATAT GGCCCCAGGG TTGGGCAGTG 2251 AGACGCGGCT GGACCGCAGC AAAGGAGATG CACGGAGCGA CACAGTTCTG 2301 CTCGACTCCT CGGCCACACT CATCACCAAT GAGGATAACG AGGAAACCGA 2351 GGGCAGCGAC GTACCTGCCG ACTACCTGCT GGGTGACGTG GAAGGTGAGG 2401 AGGACGAGCT GTACCTGCTG GATCCGGAAC ACGCGCACCC CGAGGACCCT 2451 GAGTGCGTGC TGCCGCAGGA GGCCTTTCCG CTGCGCCACG AGATCGTGGA 2501 CACGCCTCCC GGACCCGAGC ACCTGCAGGA CAAGGCCGAC TCCCCGCACG 2551 TGAGCGGCAG CGAGGCGGAT GTGGCCTCCC TGGCCCCCGT GGACTCCTCC 2601 TTCTCGCTCC TGTCTGTCTC ACACGCGCTC TACGACAGCC GCCTGCCGCC 2651 CGACTTCTTC GGCGTGCTGG TGCGCGACAT GCTGCACTTC TACGCTGAGC 2701 AGGGCGACGT GCAGATGGCT GTGTCTGTGC TCATCGTCCT GGGTGAACGG 2751 GTGCGCAAGG ACATCGACGA GCAGACCCAG GAGCACTGGT ACACTTCCTA 2801 CATCGACCTG CTGCAGCGCT TCCGCCTCTG GAACGTGTCC AACGAGGTGG 2851 TCAAGCTGAG CACCAGCCGC GCCGTCAGCT GCCTCAACCA GGCCTCCACC 2901 ACCCTGCACG TCAACTGCAG CCACTGCAAG CGGCCCATGA GCAGCCGGGG 2951 CTGGGTCTGC GACAGGTGCC ACCGCTGCGC CAGCATGTGT GCCGTCTGCC 3001 ACCACGTAGT CAAGGGTCTC TTCGTGTGGT GCCAGGGCTG CAGCCACGGC 3051 GGCCACCTGC AGCACATCAT GAAGTGGCTG GAAGGCAGCT CCCACTGTCC 3101 CGCAGGCTGC GGCCACCTCT GCGAGTACTC CTGACGGGGC ATCTGCTGGG 3151 CTTGCCCGGG CGGCCG BLAST Results Entry HS313D11 from database EMBL: Human DNA sequence from cosmid 313D11 from a contig on the short arm of chromosome 16. Contains ESTs, STS and CpG islands. Score = 6238, P = O.Oe+00, identities = 1318/1391 Medline entries No Medlme entry Peptide information for frame 3 ORF from 762 bp to 3131 bp; peptide length: 790 Category: similarity to known protein 1 MEKMSRVTTA LGGSVLTGRT MHCHLDAPAN AISVCRDAAQ VVVAGRSIFK 51 IYAIEEEQFV EKLNLRVGRK PSLNLSCADV VWHQMDENLL ATAATNGVVV 101 TWNLGRPSRN KQDQLFTEHK RTVNKVCFHP TEAHVLLSGS QDGFMKCFDL 151 RRKDSVSTFS GQSESVRDVQ FSIRDYFTFA STFENGNVQL WDIRRPDRCE 201 RMFTAHNGPV FCCDWHPEDR GWLATGGRDK MVKVWDMTTH RAKEMHCVQT 251 IAΞVARVKWR PECRHHLATC SMMVDHNIYV WDVRRPFVPA AMFEEHRDVT 301 TGIAWRHPHD PSFLLSGSKD ΞSLCQHLFRD ASQPVERANP EGLCYGLFGD 351 LAFAAKESLV AAESGRKPYT GDRRHPIFFK RKLDPAEPFA GLASSALSVF 401 ETEPGGGGMR WFVDTAERYA LAGRPLAELC DHNAKVAREL GRNQVAQTWT 451 MLRIIYCSPG LVPTANLNHS VGKGGSCGLP LMNSFNLKDM APGLGSETRL 501 DRSKGDARSD TVLLDSSATL ITNEDNEETE GSDVPADYLL GDVEGEEDEL 551 YLLDPEHAHP EDPECVLPQE AFPLRHEIVD TPPGPEHLQD KADSPHVSGS 601 EADVASLAPV DSSFSLLSVS HALYDSRLPP DFFGVLVRDM LHFYAEQGDV 651 QMAVSVLIVL GERVRKDIDE QTQEHWYTSY IDLLQRFRLW NVSNEVVKLS 701 TSRAVSCLNQ ASTTLHVNCS HCKRPMSSRG WVCDRCHRCA SMCAVCHHVV 751 KGLFVWCQGC ΞHGGHLQHIM KWLEGSSHCP AGCGHLCEYS BLASTP hits Entry YDSB_SCHPO from database SWISSPROT: HYPOTHETICAL 93.2 KD TRP-ASP REPEATS CONTAINING PROTEIN C4F8.11 IN CHROMOSOME I. >TREMBL : SPAC4F8_11 gene: "SPAC4F8.il"; product: "beta-transducm"; S. pombe chromosome I cosmid c4F8. Score = 404, P = 3.0e-42, identities = 169/639, positives = 278/639 Entry PEX7_HUMAN from database SWISSPROT: PEROXISOMAL TARGETING SIGNAL 2 RECEPTOR (PTS2 RECEPTOR) (PEROXIN-7) . >TREMBL:HSU76560_1 gene: "Pex7"; product: "peroxisome targeting signal 2 receptor"; Human peroxisome targeting signal 2 receptor (Pex7) mRNA, complete eds. >TREMBL:HSU88871_1 gene: "HsPEX7"; product: "HsPex7p"; Human HsPex7p (HsPEX7) mRNA, complete eds. Score = 220, P = l.le-15, identities = 62/244, positives = 107/244 Entry PEX7_MOUSE from database SWISSPROT: PEROXISOMAL TARGETING SIGNAL 2 RECEPTOR (PTS2 RECEPTOR) (PEROXIN-7) . >TREMBL:MMU69171_1 product: "peroxisomal PTΞ2 receptor"; Mus musculus peroxisomal PTS2 receptor mRNA, complete eds. Score = 214, P = 5.3e-15, identities = 60/240, positives = 106/240 Entry ATAC2294_7 from database TREMBL: gene: "F11P17.7"; Arabidopsis thaliana chromosome I BAC F11P17 genomic sequence, complete sequence. Score = 232, P = 3.4e-14, identities = 68/260, positives = 120/260 Entry S66835 from database PIR: probable membrane protein YOL138c - yeast (Saccharomyces cerevisiae) >TREMBL:SCYOL138C_l S. cerevisiae chromosome XV reading frame ORF YOL138C Score = 136, P = 2.5e-13, identities = 24/77, positives = 44/77 Alert BLASTP hits for DKFZphtes3_4f5, frame 3 No Alert BLASTP hits found Pedant information for DKFZphtes3_4f5, frame 3 Report for DKFZphtes3_4f5.3 [LENGTH] 790 [MW] 88207.10 [pi] 6.05 [HOMOL] SWISSPROT :YDSB_SCHPO HYPOTHETICAL 93.2 KD TRP-ASP REPEATS CONTAINING PROTEIN C4F8.11 IN CHROMOSOME I. 9e-44 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YOL138c] 5e-16 [FUNCAT] 10.04.09 regulation of g-protein activity [S. cerevisiae, YBR195c] 3e-ll [FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YBR195c] 3e-ll [FUNCAT] 03.16 dna synthesis and replication [S. cerevisiae, YBR195C] 3e-ll [FUNCAT] 09.13 biogenesis of chromosome structure [S. cerevisiae, YBR195c] 3e-ll [FUNCAT] 04.05.01.07 chromatin modification [S. cerevisiae, YBR195c] 3e-ll [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YCR072c beta-transducin family] 3e-10 [FUNCAT] 04.05.01.01 general transcription activities [S. cerevisiae, YBR198c TAF90 - TFIID subunit] 9e-09 [FUNCAT] 04.01.04 rrna processing [S. cerevisiae, YLLOllw] le-07 [FUNCAT] 30.09 organization of intracellular transport vesicles [S. cerevisiae, YDL195W] 2e 07 [FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YDL195w] 2e-07 [FUNCAT] 30.19 peroxisomal organization [S. cerevisiae, YDR142C] 4e-07 [FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YDR142c] 4e-07 [FUNCAT] 08.10 peroxisomal transport [S. cerevisiae, YDR142c] 4e-07 [FUNCAT] 08.01 nuclear transport [S. cerevisiae, YER107c] 4e-07 [FUNCAT] 04.07 rna transport [S. cerevisiae, YER107c] 4e-07 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YER107c] 4e-07 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YGL003c] 5e-07 [FUNCAT] 06.13 proteolysis [S. cerevisiae, YGL003c] 5e-07 [ FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YCR084C] 8e-07 [FUNCAT] 04.05.03 mrna processing (splicing) [S. cerevisiae, YPR178w] le-06 [FUNCAT] 03.13 meiosis [S. cerevisiae, YLR129w] 3e-06 [FUNCAT] 03.25 cytokinesis [S. cerevisiae, YCR057c] le-05 [FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YCR057c] le-05 [FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylation, farnesylation and processing) [S. cerevisiae, YEL056w] 2e-04 [FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YOR272w] 6e-04 [SCOP] dlgotb_ 2.46.3.1.1 betal-subunit of the signal-transducmg 5e-06 [PIRKW] duplication 7e-10 [PIRKW] signal transduction 7e-08 [PIRKW] peroxisome 9e-06 [PIRKW] heterotrimer 7e-08 [PIRKW] GTP binding 7e-08 [PIRKW] peroxisome biogenesis 9e-06 [PIRKW] transmembrane protein le-14 [SUPFAM] MSI1 protein 7e-10 [SUPFAM] WD repeat homology le-14 [SUPFAM] GTP-binding regulatory protein beta chain 7e-08 [SUPFAM] PRL1 protein 3e-08 [SUPFAM] coatomer complex beta' chain le-06 [PROSITE] CYTOCHROME_C 1 [PROSITE] WD_REPEATS 3 [PROSITE] MYRISTYL 10 [PROSITE] AMIDATION 2 [PROSITE] CAMP_PHOSPHO_SI E 2 [PROSITE] CK2 PHOSPHO SITE 11 [PROSITE] TYR_PHOSPHO_SITE 1 [PROSITE] PKC_PHOSPHO_SITE 7 [PROSITE] ASN_GLYCOSYLATION 4 [PFAM] WD domain, G-beta repeats [KW] All_Beta [KW] 3D [KW] LOW COMPLEXITY 2.28 % SEQ MEKMSRVTTALGGSVLTGRTMHCHLDAPANAISVCRDAAQVVVAGRSIFKIYAIEEEQFV SEG IgotB SEQ EKLNLRVGRKPSLNLSCADVVWHQMDENLLATAATNGVVVTWNLGRPSRNKQDQLFTEHK SEG IgotB TTCEEEEEETTTEEEEEET-TTTCEEE—EEECCC SEQ RTVNKVCFHPTEAHVLLSGSQDGFMKCFDLRRKDSVSTFSGQSESVRDVQFSIRDYFTFA SEG IgotB CCEEEEEEETT-TCEEEEEETTTEEEEEETTTTEEEEEECBTTCCEEEEEETTTTTEEEE SEQ STFENGNVQLWDIRRPDRCERMFTAHNGPVFCCDWHPEDRGWLATGGRDKMVKVWDMTTH SEG IgotB E-ETTTEEEEEETTTTEEEE-EEECCCCCEEEEEE-TTTTCCEEEEETTTEEEEEC.... SEQ RAKEMHCVQTIASVARVKWRPECRHHLATCSMMVDHNIYVWDVRRPFVPAAMFEEHRDVT SEG IgotB SEQ TGIAWRHPHDPSFLLSGSKDSSLCQHLFRDASQPVERANPEGLCYGLFGDLAFAAKESLV SEG IgotB SEQ AAESGRKPYTGDRRHPIFFKRKLDPAEPFAGLASSALSVFETEPGGGGMRWFVDTAERYA SEG IgotB SEQ LAGRPLAELCDHNAKVARELGRNQVAQTWTMLRIIYCSPGLVPTANLNHSVGKGGSCGLP SEG IgotB SEQ LMNSFNLKDMAPGLGSETRLDRSKGDARSDTVLLDSSATLITNEDNEETEGSDVPADYLL SEG xxxx IgotB SEQ GDVEGEEDELYLLDPEHAHPEDPECVLPQEAFPLRHEIVDTPPGPEHLQDKADSPHVSGS SEG xxxxxxxxxxxxx IgotB SEQ EADVASLAPVDSSFSLLSVSHALYDSRLPPDFFGVLVRDMLHFYAEQGDVQMAVSVLIVL SEG IgotB SEQ GERVRKDIDEQTQEHWYTSYIDLLQRFRLWNVSNEVVKLSTSRAVSCLNQASTTLHVNCS SEG IgotB SEQ HCKRPMSSRGWVCDRCHRCASMCAVCHHVVKGLFVWCQGCSHGGHLQHIMKWLEGSSHCP SEG IgotB SEQ AGCGHLCEYS SEG IgotB Prosite for DKFZphtes3_4f5.3 PS00001 74->78 ASN_GLYCOSYLATION PDOC00001 PS00001 468->472 ASN_GLYCOSYLATION PDOC00001 PS00001 691->695 ASN_GLYCOΞYLATION PDOC00001 PS00001 718->722 ASN_GLYCOSYLATION PDOC00001 PS00004 69->73 CAMP_PHOSPHO_SITE PDOC00004 PS00004 152->156 CAMP_PHOΞPHO_SITE PDOC00004 PΞ00005 17->20 PKC_PHOSPHO_SITE PDOC00005 PS00005 165->168 PKC_PHOSPHO_SITE PDOC00005 PS00005 172->175 PKC_PHOSPHO_SITE PDOC00005 PS00005 239->242 PKC_PHOSPHO_ΞITE PDOC00005 PS00005 364->367 PKC_PHOSPHO_SITE PDOC00005 PS00005 701->704 PKC PHOSPHO SITE PDOC00005 DKFZphtes3_4h6 group: intracellular transport/trafficking DKFZphtes3_4h6 encodes a novel 622 amino acid protein with strong similarity to the kinesm light chain. Kmesin is a microtubule-based motor protein that pulls vesicles or organelles towards the plus end of microtubules. Structural changes in the protein that drive motility are coupled to ATP binding and hydrolysis. The novel protein is similar to kmesin light chain, which is part of the functional kmesin holoenzyme tetrameric protein. The light chain has been proposed to function in coupling of cargo to the heavy chain or in the modulation of the ATPase activity of the heavy chain. The novel protein contains two kinesm light chain repeats and one RGD cell-attachment site. The novel kinesin protein can find application in modulating the function of kinesm and modulating intracellular transport via/on microtubules. strong similarity to Kinesm light chain complete cDNA, complete eds, start at 150, EST hits (few) Sequenced by AGOWA Locus : unknown Insert length: 2992 bp Poly A stretch at pos. 2914, polyadenylation signal at pos. 2893 1 GGCGGGATGG AGGCGGCGGG ACCGGCTCGC GGGTGCGGGT CCGGGTGAAG 51 CGGGAGGCAG CCAGAGTCGG AGCCGGGCCC GAGCACCAGG CGCAGGCCCG 101 GCGCCCGCCT GCCCGCACCC TCGTCCTCAC AGACGCCACA GCCATGGCCA 151 TGATGGTGTT TCCGCGGGAG GAGAAGCTGA GCCAGGATGA GATCGTGCTG 201 GGCACCAAGG CTGTCATCCA GGGACTGGAG ACTCTGCGTG GGGAGCATCG 251 TGCCCTGCTG GCTCCTCTGG TTGCACCTGA GGCCGGCGAA GCCGAGCCTG 301 GCTCGCAGGA GCGCTGCATC CTCCTGCGTC GCTCCCTGGA AGCCATTGAG 351 CTTGGGCTGG GGGAGGCCCA GGTGATCTTG GCATTGTCGA GCCACCTGGG 401 GGCTGTAGAA TCAGAGAAGC AGAAGCTGCG GGCGCAGGTG CGGCGTCTGG 451 TGCAGGAGAA CCAGTGGCTG CGTGAGGAGC TGGCGGGGAC ACAGCAGAAG 501 CTGCAGCGCA GTGAGCAGGC CGTGGCCCAG CTCGAGGAGG AGAAGCAGCA 551 CTTGCTGTTC ATGAGCCAGA TCCGCAAGTT GGATGAAGAC GCCTCCCCTA 601 ACGAGGAGAA GGGGGACGTC CCCAAAGACA CACTGGATGA CCTGTTCCCC 651 AATGAGGATG AGCAGAGCCC AGCCCCTAGC CCAGGAGGAG GGGATGTGTC 701 TGGTCAGCAT GGGGGCTACG AGATCCCGGC CCGGCTCCGC ACCCTGCACA 751 ACCTGGTGAT CCAATACGCC TCACAGGGCC GCTACGAGGT AGCTGTGCCA 801 CTCTGCAAGC AGGCACTCGA AGACCTGGAG AAGACGTCAG GCCACGACCA 851 CCCTGACGTT GCCACCATGC TGAACATCCT GGCACTGGTC TATCGGGATC 901 AGAACAAGTA CAAGGAGGCT GCCCACCTGC TCAATGATGC TCTGGCCATC 951 CGGGAGAAAA CACTGGGCAA GGACCACCCA GCCGTGGCTG CGACACTAAA 1001 CAACCTGGCA GTCCTGTATG GCAAGAGGGG CAAGTACAAG GAGGCTGAGC 1051 CATTGTGCAA GCGGGCACTG GAGATCCGGG AGAAGGTCCT GGGCAAGTTT 1101 CACCCAGATG TGGCCAAGCA GCTCAGCAAC CTGGCCCTGC TGTGCCAGAA 1151 CCAGGGCAAA GCTGAGGAGG TGGAATATTA CTATCGGCGG GCACTGGAGA 1201 TCTATGCTAC ACGCCTCGGG CCCGATGACC CCAATGTGGC CAAGACCAAG 1251 AACAACCTGG CTTCCTGCTA CCTGAAGCAG GGCAAGTACC AGGATGCGGA 1301 GACCTTGTAC AAGGAGATCC TCACCCGCGC TCATGAGAAA GAGTTTGGCT 1351 CTGTCAATGG GGACAACAAG CCCATCTGGA TGCACGCAGA GGAGCGGGAG 1401 GAAAGCAAGG ATAAGCGCCG GGACAGCGCC CCCTATGGGG AATACGGCAG 1451 CTGGTACAAG GCCTGTAAAG TAGACAGCCC CACAGTCAAC ACCACCCTGC 1501 GCAGCTTGGG GGCCCTATAC CGGCGCCAGG GCAAGCTGGA AGCCGCGCAC 1551 ACACTAGAGG ACTGTGCCAG CCGTAACCGC AAGCAGGGTT TGGACCCCGC 1601 AAGCCAGACC AAGGTGGTAG AACTGCTGAA AGATGGCAGT GGCAGGCGGG 1651 GAGACCGCCG CAGCAGCCGA GACATGGCTG GGGGTGCCGG GCCTCGGTCT 1701 GAGTCTGACC TCGAGGACGT GGGACCTACA GCTGAGTGGA ATGGGGATGG 1751 CAGTGGCTCC TTGAGGCGCA GCGGTTCCTT TGGGAAACTC CGGGATGCCC 1801 TGAGGCGCAG CAGTGAGATG CTGGTAAAGA AGCTGCAGGG GGGCACCCCC 1851 CAGGAGCCCC CTAACCCCAG GATGAAGCGG GCCAGTTCCC TCAACTTCCT 1901 CAACAAGAGC GTGGAAGAGC CGACCCAGCC TGGAGGCACA GGTCTCTCTG 1951 ACAGCCGCAC TCTCAGCTCC AGCTCCATGG ACCTCTCCCG ACGAAGCTCC 2001 CTGGTGGGCT AATGCTGAAG GGGCAGCCAG TCACCAGAGC GCCCACCTGG 2051 CACACCCCCC TCACCCCAGC CCTGCGCATG GGCCTGCTGC TTGTCCCGCC 2101 TGTCTCTCCC ACAGCCCCTG TCTTTTCTGT TCAATCTCAG GGTAACCTTC 2151 TCCCTTGTCA TCTCAGCCTG AGCCCTGGAG GCTGGGCCTG CCCACTCCAG 2201 CTCCATCCCT TATTTATTCC TTCCAGCAGG GCCCTCTTCC CTAGGTTCGG 2251 GCCAGCAGGA GGTGCCGGCT GGAGTCTCCA CCATAGACTC AGTGGCCTGG 2301 CCTCCCCAGA CCCCAGAGCC AAGAACACTA AGCACTCGCC GGCCCTTCGG 2351 CACCCTCGCC CTCCCTCCCG ACTCAACCCG GCCGTTGCTT CTGTATATAG 2401 AGAAATAAGT TATTGGCCGC GCGCCTCCCT TCAGTCCACG GTACTACCCG 2451 GGCCTCCCCT CGTCCCTCTT CTAGTGGTAC CGCCCAGGCC TTAATCACCC 2501 CCATTCCGTG CGGTGGTATC TCCCAGGCTC TACATTCTCG GGAGCGGCGC 2551 CTCCCAAGGG GGTCCTGGGA CCTTCTCGCG CTCCTCCTGG CCTCTGAGGG 2601 ATGCGTCCTA CCCGCGCCAT CGCCCCGTGG CCCAGGACGG GGACCTCCCC 2651 TTAGTCCGTC CTCCCACCGC CGGGCCCTGC CCCGCATCCC GGCCTTATGC 2701 ACTGCCCCTC CCACCCGGCC CCGCCCAGGC ACGGCCGACC CCGCCCCGGG 2751 CACCGCCCAC CGAGCCATCC TGCCTCGCCT CCCCCCACGC CTGCAGCTTC 2801 TCGCGAGGGG CGGCGACGGT CCCCTGGTGG CAGGAGGGGC TCCCCCTGTT 2851 GCGGGTGAGG CGGCTGCTCT CTATTTTCAG ATGTTGCTGT AGAAATAAAG 2901 ACGGTTTAAA TCTGAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 2951 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AA BLAST Results No BLAST result Medline entries 98288268: Two kinesin light chain genes in mice. Identification and characterization of the encoded proteins. Peptide information for frame 3 ORF from 144 bp to 2009 bp; peptide length: 622 Category: strong similarity to known protein Prosite motifs: RGD (502-505) KINESIN_LIGHT (223-265) KINESIN LIGHT (265-307) 1 MAMMVFPREE KLSQDEIVLG TKAVIQGLET LRGEHRALLA PLVAPEAGEA 51 EPGSQERCIL LRRSLEAIEL GLGEAQVILA LSSHLGAVES EKQKLRAQVR 101 RLVQENQWLR EELAGTQQKL QRSEQAVAQL EEEKQHLLFM SQIRKLDEDA 151 SPNEEKGDVP KDTLDDLFPN EDEQSPAPSP GGGDVSGQHG GYEIPARLRT 201 LHNLVIQYAS QGRYEVAVPL CKQALEDLEK TSGHDHPDVA TMLNILALVY 251 RDQNKYKEAA HLLNDALAIR EKTLGKDHPA VAATLNNLAV LYGKRGKYKE 301 AEPLCKRALE IREKVLGKFH PDVAKQLΞNL ALLCQNQGKA EEVEYYYRRA 351 LEIYATRLGP DDPNVAKTKN NLASCYLKQG KYQDAETLYK EILTRAHEKE 401 FGSVNGDNKP IWMHAEEREE SKDKRRDSAP YGEYGSWYKA CKVDSPTVNT 451 TLRSLGALYR RQGKLEAAHT LEDCAΞRNRK QGLDPASQTK VVELLKDGSG 501 RRGDRRSSRD MAGGAGPRSE SDLEDVGPTA EWNGDGSGSL RRSGSFGKLR 551 DALRRSSEML VKKLQGGTPQ EPPNPRMKRA SSLNFLNKSV EEPTQPGGTG 601 LSDSRTLΞSS SMDLSRRSSL VG BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_4h6, frame 3 TREMBL:AF055666_1 gene: "Klc2"; product: "kinesin light chain 2"; Mus musculus kmesin light chain 2 (Klc2) mRNA, complete eds., N = 1, Score = 2824, P = 4e-294 PIR: 153013 kinesin light chain - human, N = 1, Score = 1927, P = 4.5e-199 PIR:C41539 kinesin light chain C - rat, N = 1, Score = 1919, P = 3.2e-198 SWISSPROT :KNLC_RAT KINESIN LIGHT CHAIN (KLC) . , N = 1, Score = 1919, P = 3.2e-198 >TREMBL:AF055666_1 gene: "Klc2"; product: "kinesin light chain 2"; Mus musculus k esin light chain 2 (Klc2) mRNA, complete eds. Length = 599 HSPs: Score = 2824 (423.7 bits), Expect = 4.0e-294, P = 4.0e-294 Identities = 558/598 (93%), Positives = 572/598 (95%) Query: 1 MAMMVFPREEKLSQDEIVLGTKAVIQGLETLRGEHRALLAPLVAPEAGEAEPGSQERCIL 60 MA MV PREEKLSQDEIVLGTKAVIQGLETLRGEHRALLAPL + EAGEAEPGSQERC+L Sbjct: 1 MATMVLPREEKLSQDEIVLGTKAVIQGLETLRGEHRALLAPLASHEAGEAEPGSQERCLL 60 Query: 61 LRRSLEAIELGLGEAQVILALSSHLGAVESEKQKLRAQVRRLVQENQWLREELAGTQQKL 120 LRRSLEAIELGLGEAQVILALSSHLGAVEΞEKQKLRAQVRRLVQENQWLREELAGTQQKL Sbjct: 61 LRRSLEAIELGLGEAQVILALSSHLGAVESEKQKLRAQVRRLVQENQWLREELAGTQQKL 120 Query: 121 QRSEQAVAQLEEEKQHLLFMSQIRKLDEDASPNEEKGDVPKDTLDDLFPNEDEQSPAPSP 180 QRSEQAVAQLEEEKQHLLFMSQIRKLDE P EEKGDVPKD+LDDLFPNEDEQSPAPSP Sbjct: 121 QRSEQAVAQLEEEKQHLLFMSQIRKLDE-MLPQEEKGDVPKDSLDDLFPNEDEQSPAPSP 179 Query: 181 GGGDVSGQHGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVA 240 GGGDV+ QHGGYEI PARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVA Sbjct: 180 GGGDVAAQHGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVA 239 Query: 241 TMLNILALVYRDQNKYKEAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKE 300 TMLNILALVYRDQNKYK+AAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKE Sbjct: 240 TMLNILALVYRDQNKYKDAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKE 299 Query: 301 AEPLCKRALEIREKVLGKFHPDVAKQLSNLALLCQNQGKAEEVEYYYRRALEIYATRLGP 360 AEPLCKRALEIREKVLGKFHPDVAKQLSNLALLCQNQGKAEEVEYYYRRALEIYATRLGP Sbjct: 300 AEPLCKRALEIREKVLGKFHPDVAKQLSNLALLCQNQGKAEEVEYYYRRALEIYATRLGP 359 Query: 361 DDPNVAKTKNNLASCYLKQGKYQDAETLYKEILTRAHEKEFGSVNGDNKPIWMHAEEREE 420 DDPNVAKTKNNLASCYLKQGKYQDAETLYKEILTRAHEKEFGSVNG+NKPIWMHAEEREE Sbjct: 360 DDPNVAKTKNNLASCYLKQGKYQDAETLYKEILTRAHEKEFGSVNGENKPIWMHAEEREE 419 Query: 421 SKDKRRDSAPYGEYGSWYKACKVDSPTVNTTLRSLGALYRRQGKLEAAHTLEDCASRNRK 480 SKDKRRD P EYGSWYKACKVDSPTVNTTLR+LGALYR +GKLEAAHTLEDCASR+RK Sbjct: 420 SKDKRRDRRPM-EYGSWYKACKVDSPTVNTTLRTLGALYRPEGKLEAAHTLEDCASRSRK 478 Query: 481 QGLDPASQTKVVELLKDGSGRRGDRRSSRDMAGGAGPRSESDLEDVGPTAEWNGDGSGSL 540 QGLDPASQTKVVELLKDGSGR G RR SRD+AG P+SESDLE+ GP AEW+GDGSGSL Sbjct: 479 QGLDPASQTKVVELLKDGSGR-GHRRGSRDVAG PQSESDLEESGPAAEWSGDGSGSL 534 Query: 541 RRSGSFGKLRDALRRSSEMLVKKLQGGTPQEPPNPRMKRASSLNFLNKSVEEPTQPGG 598 RRSGSFGKLRDALRRSSEMLV+KLQGG PQEP N RMKRASSLNFLNKSVEEP QPGG Sbjct: 535 RRSGSFGKLRDALRRSSEMLVRKLQGGGPQEP-NSRMKRASSLNFLNKSVEEPVQPGG 591 Pedant information for DKFZphtes3_4h6, frame 3 Report for DKFZphtes3_4h6.3 [LENGTH] 622 [MW] 68934.82 [pi] 6.72 [HOMOL] TREMBL :AF055666_1 gene: "Klc2"; product: "kmesin light chain 2" Mus musculus kmesin light chain 2 (Klc2) mRNA, complete eds. 0.0 [BLOCKS] BL00927C Trehalase proteins [BLOCKS] BL01160I Kinesm light chain repeat proteins [BLOCKS] BL01160H Kinesm light chain repeat proteins [BLOCKS] BL01160G Kinesm light chain repeat proteins [BLOCKS] BL01160F Kinesin light chain repeat proteins [BLOCKS] BL01160E Kinesin light chain repeat proteins [BLOCKS] BL01160D Kinesin light chain repeat proteins [BLOCKS] BL01160C Kinesin light chain repeat proteins [BLOCKS] BL01160B Kinesin light chain repeat proteins [BLOCKS] BL01160A Kinesin light chain repeat proteins [SUPFAM] tetratπcopeptide repeat homology le-07 [PROSITE] RGD 1 [PROSITE] MYRISTYL 8 [PROSITE] KINESIN_LIGHT 2 [PROSITE] AMIDATION 2 [PROSITE] CAMP_PHOSPHO_SITE 5 [PROSITE] CK2_PHOSPHO_ΞI E 11 [PROSITE] TYR_PHOSPHO_SITE 3 [PROSITE] PKC_PHOSPHO_SITE 7 [PROSITE] ASN ΞLYCOSYLATION 2 [PFAM] Kmesin light chain repeat [KW] All_Alpha [KW] LOW_COMPLEXITY 12.54 % [KW] COILED COIL 4.98 % SEQ MAMMVFPREEKLSQDEIVLGTKAVIQGLETLRGEHRALLAPLVAPEAGEAEPGSQERCIL SEG PRD ccccchhhhhhhhhhhhhchhhhhhhhhhhhhhchhhhhhhhhhhhhcccccccchhhhh COILS SEQ LRRSLEAIELGLGEAQVILALSSHLGAVESEKQKLRAQVRRLVQENQWLREELAGTQQKL SEG PRD hhhhhhhhhhccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcchhhhhhhhhhhhhh COILS CCCCCCCCCCCC SEQ QRSEQAVAQLEEEKQHLLFMSQIRKLDEDASPNEEKGDVPKDTLDDLFPNEDEQSPAPSP SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhcccccccccccccccccccccccccccccccccc COILS CCCCCCCCCCCCCCCCCCC SEQ GGGDVSGQHGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVA SEG PRD cccccccccccccchhhhhhhhhhhhhhhccceeeeeehhhhhhhhhhhhhccccccchh COILS SEQ TMLNILALVYRDQNKYKEAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKE SEG xxxxxxxxxxxx PRD hhhhhhhhhhhhcchhhhhhhhhhhhhhhhhhhcccccchhhhhhhhhhhhhcccccchh COILS SEQ AEPLCKRALEIREKVLGKFHPDVAKQLSNLALLCQNQGKAEEVEYYYRRALEIYATRLGP SEG PRD hhhhhhhhhhhhhhhhcccccchhhhhhhhhhhhhhccchhhhhhhhhhhhhhhhhhccc COILS SEQ DDPNVAKTKNNLASCYLKQGKYQDAETLYKEILTRAHEKEFGSVNGDNKPIWMHAEEREE SEG xxxxx PRD ccccccchhhhhhhhhhhccchhhhhhhhhhhhhhhhhhhhccccccccchhhhhhhhhh COILS SEQ ΞKDKRRDSAPYGEYGSWYKACKVDSPTVNTTLRSLGALYRRQGKLEAAHTLEDCASRNRK SEG xxxxxxxx PRD hhhhhccccccccccccceeeeccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS SEQ QGLDPASQTKVVELLKDGSGRRGDRRSSRDMAGGAGPRSESDLEDVGPTAEWNGDGSGSL SEG xxxxxxxxxxxxxx xxxxx PRD hhccchhhhhhhhhhccccccccccccccccccccccccccccccceeeecccccccccc COILS SEQ RRSGSFGKLRDALRRSSEMLVKKLQGGTPQEPPNPRMKRASSLNFLNKSVEEPTQPGGTG SEG xxxxxxxxxx xxxx PRD ccccccchhhhhhhhhhhhhhhhhhcccccccccchhhhhhhcccccccccccccccccc COILS SEQ LSDSRTLSSSSMDLSRRSSLVG SEG xxxxxxxxxxxxxxxxxxxx.. PRD cccccccccccchhhhhhcccc COILS Prosite for DKFZphtes3_4h6.3 PS00001 449->453 ASN_GLYCOSYLATION PDOC00001 PS00001 587->591 ASN_GLYCOSYLATION PDOC00001 PS00004 425->429 CAMP_PHOSPHO_SITE PDOC00004 PΞ00004 505->509 CAMP_PHOSPHO_ΞITE PDOC00004 PS00004 554->558 CAMP_PHOSPHO_SITE PDOC00004 PS00004 578->582 CAMP_PHOSPHO_SITE PDOC00004 PS00004 616->620 CAMP_PHOSPHO_SITE PDOC00004 PS00005 30->33 PKC_PHOSPHO_SITE PDOC00005 PS00005 90->93 PKC_PHOSPHO_SITE PDOC00005 PS00005 451->454 PKC_PHOSPHO_SITE PDOC00005 PS00005 499->502 PKC_PHOSPHO_SITE PDOC00005 PS00005 507->510 PKC_PHOSPHO_SITE PDOC00005 PS00005 539->542 PKC_PHOSPHO_SITE PDOC00005 PS00005 615->618 PKC_PHOSPHO_SITE PDOC00005 PS00006 13->17 CK2_PHOSPHO_SITE PDOC00006 PS00006 151->155 CK2_PHOSPHO_SITE PDOC00006 PS00006 163->167 CK2_PHOSPHO_SITE PDOC00006 PS00006 232->236 CK2_PHOSPHO_SITE PDOC00006 PS00006 470->474 CK2_PHOSPHO_SITE PDOC00006 PS00006 507->511 CK2_PHOSPHO_SITE PDOC00006 PS00006 519->523 CK2_PHOSPHO_SITE PDOC00006 PS00006 521->525 CK2 PHOSPHO SITE PDOC00006 PS00006 568->572 CK2_PHOSPHO_SITE PDOC00006 PS00006 589->593 CK2_PHOSPHO_SITE PDOC00006 PS00006 610->614 CK2_PHOSPHO_SITE PDOC00006 PS00007 339->346 TYR_PHOSPHO_SITE PDOC00007 PS00007 339->347 TYR_PHOSPHO_SITE PDOC00007 PS00007 424->432 TYR_PHOSPHO_SITE PDOC00007 PS00008 71->77 MYRISTYL PDOC00008 PS00008 86->92 MYRISTYL PDOC00008 PS00008 182->188 MYRISTYL PDOC00008 PS00008 187->193 MYRISTYL PDOC00008 PS00008 402->408 MYRISTYL PDOC00008 PS00008 482->488 MYRISTYL PDOC00008 PS00008 598->604 MYRISTYL PDOC00008 PS00008 600->606 MYRISTYL PDOC00008 PS00009 292->296 AMIDATION PDOC00009 PS00009 499->503 AMIDATION PDOC00009 PS00016 502->505 RGD PDOC00016 PS01160 223->265 KINESIN_LIGHT PDOC00893 PS01160 265->307 KINESIN LIGHT PDOC00893 Pfam for DKFZphtes3_4h6.3 HMM_NAME Kmesin light Cham repeat HMM *RALEDREKtlGHDHPDVAtMLNNLALvCRNQNKYeEveNYYN* +ALED+EKT+GHDHPDVATMLN+LALV+R+QNKY+E++ ++N Query 223 QALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKEAAHLLN 264 50.46 265 306 1 42 dkfzphtes3_4h6.3 strong similarity to Kinesm light chain Alignment to HMM consensus : Query *RALEDREKtlGHDHPDVAtMLNNLALvCRNQNKYeEveNYYN* AL +REKTLG DHP VA LNNLA+++ ++KY+E+E + + dkfzphtes3 265 DALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCK 306 Query 348 1 42 dkfzphtes3_4h6.3 strong similarity to Kmesin light chain Alignment to HMM consensus: HMM *RALEDREKtlGHDHPDVAtMLNNLALvCRNQNKYeEveNYYN* RALE+REK+LG HPDVA++L+NLAL+C+NQ+K EEVE YY+ Query 307 RALEIREKVLGKFHPDVAKQLSNLALLCQNQGKAEEVEYYYR 348 39.10 349 390 1 42 dkfzphtes3_4h6.3 strong similarity to Kinesin light chain Alignment to HMM consensus : Query *RALEDREKtlGHDHPDVAtMLNNLALvCRNQNKYeEveNYYN* RALE+ LG D P+VA+ NNLA + Q+KY+++E +Y+ dkfzphtes3 349 RALEIYATRLGPDDPNVAKTKNNLASCYLKQGKYQDAETLYK 390 DKFZphtes3_4ol9 group: testes derived DKFZphtes3_4ol9 encodes a novel 1180 amino acid protein with weak similarity to human megakaryocyte stimulating factor and human mucin. The novel protein contains a cytochrome c family heme-bindmg site signature. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application m studying the expression profile of testis-specific genes . similarity to megakaryocyte stimulating factor and mucin complete cDNA, complete eds, EST hits (few) Sequenced by AGOWA Locus: unknown Insert length: 3767 bp Poly A stretch at pos. 3757, polyadenylation signal at pos. 3737 1 GGCTAGGTTT AGCTTCAGGG GCAGCCCAGG GCAGTGTTGC TGCATATTGC 51 ATGGATGAAA GGCTGAAGGC TGCCTCCTCT TGCAGGCTGG CTTCTGAGAT 101 TGCACCTTCT TCTCCTGCTA CTCCTCCAAA TCTATGACCC TTCAAGGCAG 151 AGCTGACCTG TCCGGTAATC AAGGCAATGC AGCCGGCCGC CTAGCTACAG 201 TTCACGAGCC AGTTGTCACC CAGTGGGCGG TGCATCCTCC AGCCCCCGCT 251 CACCCCAGTC TCCTGGACAA AATGGAGAAA GCGCCTCCAC AGCCCCAGCA 301 CGAGGGCCTC AAGTCCAAGG AGCATCTTCC GCAACAGCCT GCCGAAGGCA 351 AGACGGCGTC CCGCCGCGTC CCACGCCTCC GGGCTGTGGT CGAGAGCCAG 401 GCCTTCAAGA ACATCCTGGT AGACGAGATG GACATGATGC ACGCCCGTGC 451 AGCCACGCTC ATCCAAGCCA ACTGGAGGGG CTATTGGCTC CGGCAGAAGC 501 TGATTTCCCA GATGATGGCG GCCAAGGCCA TCCAGGAGGC CTGGCGGCGC 551 TTCAACAAGA GACACATCCT TCACTCCAGC AAGTCGTTGG TAAAGAAAAC 601 GAGGGCGGAG GAGGGGGACA TACCTTATCA CGCCCCACAG CAGGTGCGCT 651 TCCAGCATCC GGAAGAGAAC CGCCTTCTGT CCCCGCCCAT CATGGTGAAC 701 AAGGAGACCC AGTTCCCTTC CTGTGACAAT CTGGTCCTCT GCAGACCCCA 751 GTCGTCCCCC CTCCTGCAGC CCCCAGCAGC TCAGGGTACC CCAGAGCCCT 801 GTGTGCAGGG TCCTCATGCT GCCAGAGTCC GGGGGCTGGC CTTCCTGCCA 851 CACCAGACGG TCACCATCAG ATTTCCCTGC CCAGTGAGTT TGGACGCAAA 901 ATGCCAGCCA TGCCTGCTGA CCAGAACCAT CAGAAGCACC TGCCTCGTCC 951 ACATAGAGGG TGACTCAGTG AAGACCAAAC GTGTAAGTGC CCGGACCAAC 1001 AAAGCCAGGG CTCCGGAGAC ACCATTGTCC AGAAGGTATG ACCAGGCAGT 1051 TACGAGACCA TCCAGAGCCC AAACCCAGGG CCCTGTGAAA GCAGAGACCC 1101 CCAAAGCCCC CTTCCAGATA TGTCCAGGGC CCATGATCAC CAAGACTCTA 1151 CTCCAGACAT ATCCAGTGGT CTCCGTGACC CTGCCACAGA CATATCCAGC 1201 GTCCACGATG ACCACCACCC CACCCAAGAC TAGCCCAGTT CCCAAAGTAA 1251 CAATAATCAA GACCCCAGCC CAGATGTATC CGGGGCCCAC AGTGACCAAA 1301 ACTGCACCTC ACACATGCCC CATGCCCACA ATGACCAAGA TCCAGGTACA 1351 CCCCACAGCC TCCAGAACTG GCACCCCACG GCAGACATGC CCTGCGACCA 1401 TCACGGCAAA GAACCGACCT CAGGTTTCCC TTCTGGCTTC CATCATGAAG 1451 AGCCTGCCCC AGGTATGCCC GGGGCCTGCG ATGGCAAAGA CCCCACCCCA 1501 GATGCACCCG GTCACCACCC CAGCCAAAAA CCCATTGCAA ACATGTCTGT 1551 CAGCCACAAT GTCCAAGACT TCATCCCAGA GGAGCCCAGT TGGGGTGACC 1601 AAGCCCTCAC CCCAGACCCG CCTGCCAGCC ATGATAACCA AGACCCCAGC 1651 CCAGTTACGC TCGGTGGCCA CCATCCTCAA GACTCTGTGT CTGGCCTCTC 1701 CAACAGTGGC AAATGTCAAG GCTCCACCCC AAGTGGCGGT AGCAGCCGGA 1751 ACTCCCAACA CCTCAGGCTC CATCCATGAG AACCCACCCA AGGCCAAGGC 1801 CACCGTGAAT GTGAAGCAGG CTGCAAAGGT GGTGAAAGCC TCATCCCCCT 1851 CCTATTTGGC TGAGGGGAAG ATCAGGTGCC TGGCTCAACC ACATCCGGGA 1901 ACTGGGGTCC CCAGGGCTGC AGCTGAGCTT CCTTTGGAAG CCGAGAAAAT 1951 CAAGACTGGC ACCCAGAAAC AGGCGAAAAC AGACATGGCA TTTAAGACCA 2001 GTGTGGCAGT GGAAATGGCT GGGGCTCCAT CCTGGACAAA AGTTGCTGAG 2051 GAAGGGGACA AGCCACCTCA CGTGTATGTG CCTGTAGACA TGGCTGTCAC 2101 CCTGCCCCGG GGACAGCTGG CTGCCCCACT GACCAATGCC TCATCCCAGA 2151 GACATCCACC CTGCCTGTCC CAGAGACCAC TGGCCGCCCC GCTGACCAAG 2201 GCCTCATCTC AGGGACATCT GCCCACTGAG CTGACCAAGA CCCCATCCCT 2251 GGCCCATCTG GACACCTGTC TGAGCAAGAT GCATTCCCAG ACACATCTGG 2301 CCACAGGTGC CGTGAAGGTC CAGTCCCAAG CGCCTCTAGC CACCTGTCTG 2351 ACCAAGACGC AGTCCCGGGG GCAGCCGATC ACAGACATAA CCACGTGCCT 2401 CATCCCAGCG CACCAGGCTG CTGATCTCAG CAGCAACACC CACTCCCAGG 2451 TGCTCCTAAC AGGGTCCAAG GTGTCCAACC ACGCCTGCCA GCGCCTCGGT 2501 GGCCTCAGCG CCCCACCCTG GGCCAAGCCA GAGGACAGAC AGACCCAGCC 2551 ACAGCCCCAC GGACACGTGC CGGGGAAGAC CACTCAGGGG GGACCATGCC 2601 CGGCAGCCTG TGAGGTCCAG GGTATGCTGG TGCCGCCGAT GGCACCCACC 2651 GGCCATTCCA CATGCAACGT TGAGTCCTGG GGAGACAACG GAGCCACACG 2701 TGCCCAGCCA TCAATGCCCG GCCAGGCGGT GCCCTGCCAG GAGGACACGG 2751 GCCCCGCGGA CGCTGGTGTG GTTGGTGGCC AATCGTGGAA CCGCGCATGG 2801 GAGCCAGCCA GGGGTGCTGC GTCCTGGGAC ACCTGGCGCA ACAAGGCGGT 2851 GGTGCCTCCC AGGCGGTCCG GGGAGCCAAT GGTGTCCATG CAGGCTGCAG 2901 AGGAGATCCG CATCCTCGCA GTGATCACTA TCCAGGCGGG CGTCCGTGGC 2951 TACCTGGCGC GTCGCAGGAT CCGGCTGTGG CACCGGGGGG CCATGGTCAT 3001 CCAAGCTACT TGGCGCGGCT ACCGTGTGCG GCGGAACCTG GCACACCTCT 3051 GCAGAGCCAC CACGACCATC CAGTCTGCCT GGCGCGGCTA CAGCACCCGC 3101 CGGGACCAAG CCCGGCACTG GCAGATGCTC CACCCCGTCA CGTGGGTGGA 3151 GCTGGGCAGC CGGGCCGGGG TCATGTCTGA CCGAAGCTGG TTCCAGGATG 3201 GCAGAGCCAG GACAGTATCT GACCATCGCT GCTTCCAGTC CTGCCAGGCA 3251 CACGCTTGCA GCGTCTGCCA CTCCCTGAGC TCCAGGATCG GGAGCCCGCC 3301 CAGCGTGGTG ATGCTAGTGG GCTCCAGCCC TCGCACCTGT CATACCTGTG 3351 GACGCACACA GCCCACCCGT GTGGTGCAGG GCATGGGCCA GGGCACTGAG 3401 GGCCCCGGGG CAGTGTCTTG GGCCTCCGCC TACCAGCTGG CTGCCCTGAG 3451 TCCCAGGCAG CCGCATCGCC AGGACAAAGC GGCCACAGCC ATCCAGTCCG 3501 CCTGGAGGGG CTTTAAGATC CGCCAGCAGA TGAGGCAGCA GCAAATGGCA 3551 GCGAAGATAG TTCAAGCCAC CTGGCGAGGC CACCATACCC GGAGCTGTCT 3601 GAAGAACACA GAGGCGCTCT TGGGACCAGC AGACCCCTCG GCCAGCTCAC 3651 GGCACATGCA TTGGCCTGGC ATCTAGGACC CTGGCTCCCT GCAGTGGGGA 3701 CTTCGTGGGA GGCACTCATG GCTCTCTGGG TCTAATGAAT AAAGTCCTCC 3751 ACAGCCTAAA AAAAAAA BLAST Results No BLAST result Medline entries No Medl e entry Peptide information for frame 2 ORF from 134 bp to 3673 bp; peptide length: 1180 Category: similarity to known protein 1 MTLQGRADLS GNQGNAAGRL ATVHEPVVTQ WAVHPPAPAH PSLLDKMEKA 51 PPQPQHEGLK SKEHLPQQPA EGKTASRRVP RLRAVVESQA FKNILVDEMD 101 MMHARAATLI QANWRGYWLR QKLISQMMAA KAIQEAWRRF NKRHILHSΞK 151 SLVKKTRAEE GDIPYHAPQQ VRFQHPEENR LLSPPIMVNK ETQFPSCDNL 201 VLCRPQSSPL LQPPAAQGTP EPCVQGPHAA RVRGLAFLPH QTVTIRFPCP 251 VSLDAKCQPC LLTRTIRSTC LVHIEGDSVK TKRVSARTNK ARAPETPLSR 301 RYDQAVTRPS RAQTQGPVKA ETPKAPFQIC PGPMITKTLL QTYPVVSVTL 351 PQTYPASTMT TTPPKTSPVP KVTIIKTPAQ MYPGPTVTKT APHTCPMPTM 401 TKIQVHPTAS RTGTPRQTCP ATITAKNRPQ VSLLASIMKS LPQVCPGPAM 451 AKTPPQMHPV TTPAKNPLQT CLSATMSKTS SQRSPVGVTK PSPQTRLPAM 501 ITKTPAQLRS VATILKTLCL ASPTVANVKA PPQVAVAAGT PNTSGSIHEN 551 PPKAKATVNV KQAAKVVKAS SPSYLAEGKI RCLAQPHPGT GVPRAAAELP 601 LEAEKIKTGT QKQAKTDMAF KTSVAVEMAG APSWTKVAEE GDKPPHVYVP 651 VDMAVTLPRG QLAAPLTNAS SQRHPPCLSQ RPLAAPLTKA SSQGHLPTEL 701 TKTPSLAHLD TCLSKMHSQT HLATGAVKVQ SQAPLATCLT KTQSRGQPIT 751 DITTCLIPAH QAADLSSNTH SQVLLTGSKV SNHACQRLGG LSAPPWAKPE 801 DRQTQPQPHG HVPGKTTQGG PCPAACEVQG MLVPPMAPTG HSTCNVESWG 851 DNGATRAQPS MPGQAVPCQE DTGPADAGVV GGQSWNRAWE PARGAASWDT 901 WRNKAVVPPR RSGEPMVSMQ AAEEIRILAV ITIQAGVRGY LARRRIRLWH 951 RGAMVIQATW RGYRVRRNLA HLCRATTTIQ SAWRGYSTRR DQARHWQMLH 1001 PVTWVELGSR AGVMSDRSWF QDGRARTVΞD HRCFQSCQAH ACSVCHSLΞS 1051 RIGSPPΞVVM LVGSSPRTCH TCGRTQPTRV VQGMGQGTEG PGAVSWASAY 1101 QLAALSPRQP HRQDKAATAI QSAWRGFKIR QQMRQQQMAA KIVQATWRGH 1151 HTRSCLKNTE ALLGPADPSA SSRHMHWPGI BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_4ol9, frame 2 TREMBL:HSU70136_1 product: "megakaryocyte stimulating factor"; Human megakaryocyte stimulating factor mRNA, complete eds., N = 2, Score = 242, P = 9.6e-16 TREMBL : HSMUC2A_1 gene: "MUC2"; product: "mucin"; Human mucιn-2 gene, partial eds., N = 1, Score = 204, P = 1.4e-12 PIR:S48478 glucan 1, 4-alpha-glucosιdase (EC 3.2.1.3) - yeast (Saccharomyces cerevisiae), N = 1, Score = 192, P = 9.6e-ll >TREMBL:HSU70136_1 product: "megakaryocyte stimulating factor"; Human megakaryocyte stimulating factor mRNA, complete eds. Length = 1, 404 HSPs: Score = 242 (36.3 bits), Expect = 9.6e-16, Sum P(2) = 9.6e-16 Identities = 145/546 (26%), Positives = 198/546 (36%) Query: 282 KRVSARTNKARAPETPLSRRYDQAVTRPSRAQTQGPVKAETPKAPFQIC-PGPMITKTLL 340 K+ + T K AP TP PS + P T AP P P TK+ Sbjct: 488 KKPAPTTPKEPAPTTP-KEPAPTTTKEPSPTTPKEPAPTTTKSAPTTTKEPAPTTTKSAP 546 Query: 341 QTYPVVSVTLPQ TYPASTMTTTPPKTSPV-PKVTIIKTPAQMYPGPTVTKTAPHTC 395 T S T + T P TTP K +P PK TP + P PT TK Sbjct: 547 TTPKEPSPTTTKEPAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKE—PAPTTTKK 599 Query: 396 PMPTMTKIQVHPTASRTGTPRQTCPATITAKNRPQVSLLASIMKSLPQVCPGPAMAKTPP 455 P PT K + PT TP++T P T LA P +A T P Sbjct: 600 PAPTAPK-EPAPT TPKETAPTTPKKLTPTTPEKLAPTTPEKPAPTTPEELAPTTP 653 Query: 456 QMHPVTTPAKNPLQTCLSATMSKTSSQRSPVGVTKPSPQT-RLPAMIT-KTPAQLRSVAT 513 + TTP + P T A T + +P +P+P T + PA T K A T Sbjct: 654 EEPTPTTP-EEPAPTTPKAAAPNTPKEPAPTTPKEPAPTTPKEPAPTTPKETAPTTPKGT 712 Query: 514 ILKTLCLASPTVANVKAPPQVAVAAG TPNTSGSIHENPPKAKATVNVKQAAKVV-KA 569 TL +PT AP ++A T TS PK A K+ A K Sbjct: 713 APTTLKEPAPTTPKKPAPKELAPTTTKEPTSTTΞDKPAPTTPKGTAPTTPKEPAPTTPKE 772 Query: 570 SSPSYLAEGKIRCLAQPHPGTGVPRAAAELPLEAEKIKTGT—QKQAKTDMAFKTSVAVE 627 +P+ L +P P T A EL K T T K A T +T+ Sbjct: 773 PAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTTTKGPTSTTSDKPAPTTPK-ETAPTTP 831 Query: 628 MAGAPSWTKVAEEGDKPPHVYVPVDMAVTLPRGQLAAPLTNASSQRHPPCLSQRPLAAPL 687 AP+ K + P P V+ P + S P LS P L Sbjct: 832 KEPAPTTPK—KPAPTTPETPPPTTΞEVSTPTTTKEPTTIHKSPDESTPELSAEPTPKAL 889 Query: 688 TKASSQGHLPTELTKTPSLA--HLDTCLSKMHSQTHLATGAVKVQSQAPLAT—CLTKTQ 743 + + +PT TKTP+ + T ++ L T + + AP T T T+ Sbjct: 890 ENSPKEPGVPT—TKTPAATKPEMTTTAKDKTTERDLRT-TPETTTAAPKMTKETATTTE 946 Query: 744 SRGQPITDITTCLIPAHQAADLS—SNTHSQVLLTGSKVSN—HACQRLGGLSAPP-WAK 798 + TT + + D + T + KV+ ++ P AK Sbjct: 947 KTTESKITATTTQVTSTTTQDTTPFKITTLKTTTLAPKVTTTKKTITTTEIMNKPEETAK 1006 Query: 799 PEDRQTQPQPHGHVPGKTTQGGPCPAA 825 P+DR T + P K T+ P + Sbjct: 1007 PKDRATNSKATTPKPQKPTKAPKKPTS 1033 Score = 205 (30.8 bits), Expect = 3. le-12. Sum P(2) = 3. le-12 Identities = 146/565 (25%), Positives = 209/565 (36%) Query: 281 TKRVSARTNKARAPETPLSRRYDQAVTRPSRAQTQGPVKAE—TPKAPFQICPGPMITKT 338 TK+ + K AP TP + A T P + P K TP+ P P + T Sbjct: 597 TKKPAPTAPKEPAPTTPK ETAPTTPKKLTPTTPEKLAPTTPEKPAPTTPEELAPTT 652 Query: 339 LLQTYPVVSVTLPQTYPASTMTTTPPKTSPV-PKVTIIKTPAQMYPGPTVTK-TAPHTCP 396 + P T P + TP + +P PK TP + P PT K TAP T P Sbjct: 653 PEEPTPTTPEEPAPTTPKAAAPNTPKEPAPTTPKEPAPTTPKE—PAPTTPKETAP-TTP 709 Query: 397 M PTMTKIQVHPTASRTGTPRQTCPATITAKNRPQVSLLASIMKSLPQVCPGPAMAKT 453 PT K + PT + P++ P T + S + K P G A T Sbjct: 710 KGTAPTTLK-EPAPTTPKKPAPKELAPTT TKEPTSTTSD--KPAPTTPKGTAPT-T 761 Query: 454 PPQMHPVTTPAKNPLQTCLSATMSKTSSQRSPVGVTKPSPQTRLPAMITKTPAQLRSVAT 513 P + P TTP K P T T T + +P KP+P+ P TK P S Sbjct: 762 PKEPAP-TTP-KEPAPTTPKGTAPTTLKEPAPTTPKKPAPKELAPTT-TKGPTSTTSDKP 818 Query: 514 ILKTLCLASPTVANVKAPPQVAVAAGTPNTSGSIHENPPKAKATVNV KQAAKVVKA 569 T +PT AP A P T E PP + V+ K+ + K+ Sbjct: 819 APTTPKETAPTTPKEPAPTTPKKPA—PTTP ETPPPTTSEVSTPTTTKEPTTIHKS 872 Query: 570 SSPSYLAEGKIRCLAQPHPGTGVPRAAAELPLEAEKIKTGTQKQAKTDMAFKTSVAV 626 S+P AE + L GVP + P + T T K T+ +T+ Sbjct: 873 PDESTPELSAEPTPKALENSPKEPGVP—TTKTPAATKPEMTTTAKDKTTERDLRTTPET 930 Query: 627 EMAGAPSWTK-VAEEGDKPPHVYVPVDMAVTLPRGQLAAPLTNASSQRHPPCLSQRPLAA 685 A AP TK A +K + +T Q+ + T ++ L LA Sbjct: 931 TTA-APKMTKETATTTEKT TESKITATTTQVTSTTTQDTTPFKITTLKTTTLAP 983 Query: 686 PLTKASSQGHLPTELTKTPSLAHLDTCLSKMHSQTHLATGAVKVQS QAPLATCLT 740 +T + + TE+ P +T K + AT K Q + P +T Sbjct: 984 KVT-TTKKTITTTEIMNKPE ETAKPKDRATNSKAT-TPKPQKPTKAPKKPTSTKKP 1037 Query: 741 KTQSR-GQPITDIT TCLIPAHQAADLSΞNTHSQVLLTGSKVSNHACQRLGGLSAPP 795 KT R +P T T T +P + Q ++ N + S Sbjct: 1038 KTMPRVRKPKTTPTPRKMTSTMPELNPTSRIAEAMLQTTTRPNQTPNSKLVEVNPKSEDA 1097 Query: 796 W-AKPEDRQTQPQPHGHVPGKTTQGGPCPAACEVQGMLVPPMAPTGHSTCN 845 A+ E +PH +P T P QG+++ PM + CN Sbjct: 1098 GGAEGETPHMLLRPHVFMPEVTPDMDYLPRVPN-QGIIINPMLΞDETNICN 1147 Score = 198 (29.7 bits), Expect = 2.3e-ll, Sum P(2) = 2.3e-ll Identities = 142/513 (27%), Positives = 200/513 (38%) Query: 204 RPQSSPLLQPPAAQGTPEPCVQGPHAARVRGLAFLPHQTVTIRFPCPVSLDAKCQPCLLT 263 R + P +PP G + H V+ + +P L Sbjct: 207 RTKKKPTPKPPVVDEAGSGLDNGDFKVTTPDTSTTQHNKVSTSPKITTAKPINPRPΞLPP 266 Query: 264 R—TIRSTCLVHIEGDΞVKTKRVSARTNKARAP ETPLSRRYDQAVTRPSR AQTQ 315 T + T L + +V+TK + TNK + E S + Q++ + S A T Sbjct: 267 NSDTSKETSLTVNKETTVETKETTT-TNKQTSTDGKEKTTSAKETQSIEKTSAKDLAPTS 325 Query: 316 GPVKAETPKAPFQICPGPMITKTLLQTYPVVSVTLPQTYPASTMTTTPPKTSPVPKVTII 375 + TPKA GP +T T + P T P+ PAST TP + +P + Sbjct: 326 KVLAKPTPKAE-TTTKGPALT-TPKEPTP TTPKE-PAST TPKEPTPTTIKSAP 375 Query: 376 KTPAQMYPGPTVTKTAPHTC—PMPTMTKIQVHPTASRTGTPRQTC-PATITAKNRPQVS 432 TP + P PT TK+AP T P PT TK + PT + P T PA T K+ P Sbjct: 376 TTPKE—PAPTTTKSAPTTPKEPAPTTTK-EPAPTTPKEPAPTTTKEPAPTTTKSAPTTP 432 Query: 433 LLASIMKSLPQVCPGPAMAKTPPQMHPVTTPAKNPLQTCLSATMSKTSSQRSPVGVT 489 + K P PA TP + P TTP K P T + T + +P Sbjct: 433 KEPAPTTPKKPAPTTPKEPAPT-TPKEPTP-TTP-KEPAPTTKEPAPT-TPKEPAPTAPK 488 Query: 490 KPSPQT-RLPAMIT-KTPAQLRSVA TILK TLCLASPTVANVKAPPQVAVAAGT 540 KP+P T + PA T K PA + T K T ++PT AP A T Sbjct: 489 KPAPTTPKEPAPTTPKEPAPTTTKEPSPTTPKEPAPTTTKSAPTTTKEPAPTTTKSAPTT 548 Query: 541 PNT-SGSIHENP PKAKATVNVKQAAKVV-KASSPSYLAEGKIRCLAQPHPGTGVPR 594 P S + + P PK A K+ A K +P+ E +P P P+ Sbjct: 549 PKEPSPTTTKEPAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPAPTTTKKPAPTA--PK 606 Query: 595 AAAELPLEAEKIKTGTQKQAKTDMAFKTSVAVEMAGAPSWTK-VAEEGDKPPHVYVPVDM 653 A ' P ++ T K+ K + AP+ + +A + P P + Sbjct: 607 EPA—PTTPKETAPTTPKKLTPTTPEKLAPTTPEKPAPTTPEELAPTTPEEPTPTTPEEP 664 Query: 654 AVTLPRGQLAAPLTNASSQRHP-PCLSQRPLAAPLTKASSQGHLPTELTKTPSLAHLDTC 712 A T P+ AAP T + P P + P AP T P E T T Sbjct: 665 APTTPKA—AAPNT PKEPAPTTPKEP—APTTPKEPAPTTPKETAPTTPKGTAPTT 716 Query: 713 LSK 715 L + Sbjct: 717 LKE 719 Score = 108 (16.2 bits). Expect = 4.3e-02, Sum P(2) = 4.3e-02 Identities = 60/214 (28%), Positives = 85/214 (39%) Query: 265 TIRSTCLVHIEGDSVKTKRVSAR-TNKA—RAPETP-LSRRYDQAVTRPSRAQTQGPVKA 320 T + +H D T +SA T KA +P+ P + A T+P T Sbjct: 862 TTKEPTTIHKSPDE-STPELSAEPTPKALENSPKEPGVPTTKTPAATKPEMTTTAKDKTT 920 Query: 321 ETP—KAPFQICPGPMITK-TLLQTYPVVSVTLPQTYPASTMTTTPPKTSPVPKVTIIKT 377 E P P +TK T T + T T TTT T+P K+T +KT Sbjct: 921 ERDLRTTPETTTAAPKMTKETATTTEKTTESKITATTTQVTSTTTQD-TTPF-KITTLKT 978 Query: 378 PAQMYPGPTVTK TAPHTCPMPTMT-KIQVHPTASRTGTPRQTCPATITAKNRPQVSL 433 + P T TK T P T K + T S+ TP+ P A +P + Sbjct: 979 TT-LAPKVTTTKKTITTTEIMNKPEETAKPKDRATNSKATTPKPQKPTK—APKKPTSTK 1035 Query: 434 LASIMKSL—PQVCPGPA-MAKTPPQMHPVTTPAKNPLQT 470 M + P+ P P M T P+++P + A+ LQT Sbjct: 1036 KPKTMPRVRKPKTTPTPRKMTSTMPELNPTSRIAEAMLQT 1075 Score = 56 (8.4 bits), Expect = 3. le-12, Sum P(2) = 3. le-12 Identities = 17/60 (28%), Positives = 22/60 (36%) Query: 22 TVHEPVVTQWAVHPPAPAHPSLLDKMEKAPPQPQHEGLKS-KEHLPQQPAEGKTASRRVP 80 T EP T P P PS E AP P+ + K+ P P E + + P Sb j ct: 533 TTKEPAPTTTKSAPTTPKEPSPTTTKEPAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEP 592 Score = 52 (7.8 bits), Expect = 9.6e-16, Sum P(2) = 9.6e-16 Identities = 17/59 (28%), Positives = 22/59 (37%) Query: 22 TVHEPV-VTQWAVHPPAPAHPSLLDKMEKAPPQPQHEGLKSKEHLPQQPAE-GKTAΞRR 78 T EP T P P P+ E P P+ +KE P P E TA ++ Sbjct: 431 TPKEPAPTTPKKPAPTTPKEPAPTTPKEPTPTTPKEPAPTTKEPAPTTPKEPAPTAPKK 489 Score = 51 (7.7 bits), Expect = 1.2e-15, Sum P(2) = 1.2e-15 Identities = 15/51 (29%), Positives = 19/51 (37%) Query: 22 TVHEPVVTQWAVHPPAPAHPSLLDKMEKAPPQPQHEGLKS-KEHLPQQPAE 71 T EP T P P P+ + AP P+ + KE P P E Sbjct: 416 TTKEPAPTTTKSAPTTPKEPAPTTPKKPAPTTPKEPAPTTPKEPTPTTPKE 466 Score = 47 (7.1 bits), Expect = 3.2e-15, Sum P(2) = 3.2e-15 Identities = 12/41 (29%), Positives = 17/41 (41%) Query: 36 PAPAHPSLLDKMEKAPPQPQHEGLKSKEHLPQQPAEGKTAS 76 P P P + P +P +KS P++PA T S Sbjct: 350 PTPTTPK—EPASTTPKEPTPTTIKSAPTTPKEPAPTTTKS 388 Score = 47 (7.1 bits), Expect = 3.2e-15, Sum P(2) = 3.2e-15 Identities = 15/57 (26%), Positives = 19/57 (33%) Query: 22 TVHEPVVTQWAVHPPAPAHPSLLDKMEKAPPQPQHEG-LKSKEHLPQQPAEGKTASR 77 T EP T P P P+ E AP P+ +KE P T + Sbjct: 377 TPKEPAPTTTKΞAPTTPKEPAPTTTKEPAPTTPKEPAPTTTKEPAPTTTKSAPTTPK 433 Score = 46 (6.9 bits), Expect = 4.0e-15, Sum P(2) = 4.0e-15 Identities = 16/58 (27%), Positives = 22/58 (37%) Query: 20 LATVHEPVVT QWAVHPPAPAHPSLLDKMEKAPPQPQHEGLKSKEHLPQQPAEGKT 74 L T EP T + A P P+ + P +P KS P++PA T Sbjct: 344 LTTPKEPTPTTPKEPASTTPKEPTPTTIKSAPTTPKEPAPTTTKSAPTTPKEPAPTTT 401 Score = 42 (6.3 bits), Expect = 1.0e-14, Sum P(2) = 1.0e-14 Identities = 15/60 (25%), Positives = 21/60 (35%) Query: 22 TVHEPVVTQWAVHPPAPAHPSLLDKMEKAPPQPQHEGLKS-KEHLPQQPAEGKTAΞRRVP 80 T EP T P P P+ + AP P+ + KE P E + + P Sbjct: 463 TPKEPAPTTKEPAPTTPKEPAPTAPKKPAPTTPKEPAPTTPKEPAPTTTKEPSPTTPKEP 522 Score = 39 (5.9 bits), Expect = 2. le-14, Sum P(2) = 2. le-14 Identities = 15/55 (27%), Positives = 20/55 (36%) Query: 22 TVHEPVVTQWAVHPPAPAHPΞLLDKMEKAPPQPQHEGLKSKEHLPQQPAEGKTAΞ 76 T EP T P PA + + P +P KS ++PA T S Sbjct: 494 TPKEPAPTT PKEPAPTTTKEPSPTTPKEPAPTTTKSAPTTTKEPAPTTTKS 544 Pedant information for DKFZphtes3_4ol9, frame 2 Report for DKFZphtes3_4ol9.2 [LENGTH] 1180 [MW] 127693.40 [pi] 10.25 [HOMOL] SWISSPROT :MUC2_HUMAN MUCIN 2 PRECURSOR (INTESTINAL MUCIN 2). le-08 [FUNCAT] 98 classification not yet clear-cut [S. cerevisiae, YJR151C] 6e-06 [FUNCAT] 30.01 organization of cell wall [S. cerevisiae, YIR019c] 6e-06 [FUNCAT] 30.90 extracellular/secretion proteins [S. cerevisiae, YIR019c] 6e-06 [FUNCAT] 01.05.01 carbohydrate utilization [S. cerevisiae, YIR019c] 6e-06 [BLOCKS] BL00412B Neuromodulin (GAP-43) proteins [PROSITE] CYTOCHROME_C 1 [PROSITE] MYRISTYL 12 [PROSITE] CAMP_PHOSPHO_SITE 1 [PROSITE] CK2_PHOSPHO_SITE 8 [PROSITE] PKC_PHOSPHO_SITE 25 [PROSITE] ASNJΞLYCOSYLATION 2 [KW] Alpha_Beta [KW] LOW COMPLEXITY 5.00 % SEQ MTLQGRADLSGNQGNAAGRLATVHEPWTQWAVHPPAPAHPSLLDKMEKAPPQPQHEGLK SEG PRD cccccceeeccccccceeeeeeeeceeeeeeeecccccccceeeeccccccccccccccc SEQ ΞKEHLPQQPAEGKTASRRVPRLRAVVESQAFKNILVDEMDMMHARAATLIQANWRGYWLR SEG PRD cccccccccccccccccchhhhhhhhhhhhhhheeehhhhhhhhhhhhhhhhhccchhhh SEQ QKLISQMMAAKAIQEAWRRFNKRHILHSSKSLVKKTRAEEGDIPYHAPQQVRFQHPEENR SEG PRD hhhhhhhhhhhhhhhhhhhhhhheeeecccchhhhhhhhcccccccccceeeecccccce SEQ LLSPPIMVNKETQFPSCDNLVLCRPQSSPLLQPPAAQGTPEPCVQGPHAARVRGLAFLPH SEG PRD eeccceeeecccccccccceeeecccccccccccccccccccccccccceeeeeeeeccc SEQ QTVTIRFPCPVSLDAKCQPCLLTRTIRSTCLVHIEGDSVKTKRVSARTNKARAPETPLSR SEG PRD eeeeeecccccccccccccccccccccceeeeecccccccceeeeecccccccccccccc SEQ RYDQAVTRPSRAQTQGPVKAETPKAPFQICPGPMITKTLLQTYPVVSVTLPQTYPASTMT SEG xxxx PRD ccceeeeeccccccccceeecccccccccccccccccccccccccccccccccccccccc SEQ TTPPKTSPVPKVTIIKTPAQMYPGPTVTKTAPHTCPMPTMTKIQVHPTAΞRTGTPRQTCP SEG xxxxxxxxxxxxx PRD cccccccccccceeeccccccccccccccccccccccccccceeeccccccccccccccc SEQ ATITAKNRPQVSLLASIMKSLPQVCPGPAMAKTPPQMHPVTTPAKNPLQTCLSATMSKTS SEG PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ SQRSPVGVTKPSPQTRLPAMITKTPAQLRSVATILKTLCLASPTVANVKAPPQVAVAAGT SEG PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ PNTSGSIHENPPKAKATVNVKQAAKVVKASSPSYLAEGKIRCLAQPHPGTGVPRAAAELP SEG xxxxxxxxxxxxxxxxx xxxxxx PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ LEAEKIKTGTQKQAKTDMAFKTSVAVEMAGAPSWTKVAEEGDKPPHVYVPVDMAVTLPRG SEG xxxx PRD ccccccccccccccccccccccccccccccccccceeeeccccccceeeccccccccccc SEQ QLAAPLTNASSQRHPPCLSQRPLAAPLTKASSQGHLPTELTKTPSLAHLDTCLSKMHSQT SEG PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ HLATGAVKVQSQAPLATCLTKTQSRGQPITDITTCLIPAHQAADLSSNTHSQVLLTGSKV SEG PRD ccccceeeeeccccceeeeccccccccccccccccccccccccccccccceeeeeccccc SEQ SNHACQRLGGLSAPPWAKPEDRQTQPQPHGHVPGKTTQGGPCPAACEVQGMLVPPMAPTG SEG PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ HSTCNVESWGDNGATRAQPSMPGQAVPCQEDTGPADAGVVGGQSWNRAWEPARGAASWDT SEG PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ WRNKAVVPPRRSGEPMVSMQAAEEIRILAVITIQAGVRGYLARRRIRLWHRGAMVIQATW SEG PRD ccceeecccccccccchhhhhhhhhhhhhhhhccccchhhhhhhhhhhhhhhhhhhhhhh SEQ RGYRVRRNLAHLCRATTTIQSAWRGYSTRRDQARHWQMLHPVTWVELGSRAGVMSDRSWF SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhheeeeeccchhhhhhhhhhh SEQ QDGRARTVSDHRCFQSCQAHACSVCHSLSSRIGSPPSVVMLVGSSPRTCHTCGRTQPTRV SEG PRD hccceeeeccceeeecccceeeeeeeecccccccccceeeeeecccccccccccccccee SEQ VQGMGQGTEGPGAVSWASAYQLAALSPRQPHRQDKAATAIQΞAWRGFKIRQQMRQQQMAA SEG xxxxxxxxxxxxx PRD eeeccccccccccchhhhhhhhhhhhhccccchhhhhhhhhhhhhhhhhhhhhhhhhhhh SEQ KIVQATWRGHHTRSCLKNTEALLGPADPSASSRHMHWPGI SEG XX PRD hhhhhhhccccccchhhhhhhhcccccccccccccccccc cocn w en en w ω en en ω co co co ω ω c cn ω w en en w w w co en w cn cn w co en ω en w 0000000000000000000000000000000000000000000000000 0000000000000000000000000000000000000000000000000 t-'OO OOOO OOOO OOOOOOOOOOO OOOOOOOOOOOOOOO OOOOO OOO O O OO VDO O OOO O OOO O OOO O OOOOOO O OOOO O OOO OO OOO O O OOOO O OOO O OOO DKFZphtes3_50j4 group: testes derived DKFZphtes3_50j4 encodes a novel 187 ammo acid protein proline rich protein. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application m studying the expression profile of testis-specific genes . unknown, prolin ritch protein complete cDNA, complete eds, EST hits Sequenced by DKFZ Locus : unknown Insert length: 1186 bp Poly A stretch at pos. 1176, polyadenylation signal at pos. 1126 1 CACTGGGCGT CTGAAGCTCA GAGCTCACCC CTGAGATGGG CTCTCCTAGG 51 CCTCCTGGGA TGAGGGAGCC ACCAGGACCC AGTGCTGTGA TGCCTGCTCT 101 TCCCTCTACC AGCACCTGCC CGCCCAGAGA CCAGGGCACC CCTGAAGTCC 151 AGCCCACCCC TGCAAAGGAC ACATGGAAGG GCAAGCGGCC TCGATCCCAG 201 CAGGAGAACC CAGAGAGCCA GCCTCAGAAG AGGCCACGCC CCTCAGCCAA 251 GCCCTCCGTC GTAGCTGAGG TCAAGGGCAG CGTCTCGGCC AGCGAACAGG 301 GCACCTTGAA TCCCACGGCT CAAGACCCCT TCCAGCTCTC CGCTCCTGGC 351 GTCTCCTTGA AGGAGGCTGC AAATGTTGTG GTCAAGTGCC TCACCCCTTT 401 CTACAAGGAG GGCAAGTTTG CTTCCAAGGA GTTGTTTAAA GGCTTTGCCC 451 GCCACCTCTC ACACTTGCTG ACTCAGAAGA CCTCTCCTGG AAGGAGCGTG 501 AAAGAAGAGG CCCAGAACCT CATCAGGCAC TTCTTCCATG GCCGGGCCCG 551 GTGCGAGAGC GAAGCTGACT GGCATGGCCT GTGTGGCCCC CAGAGATGAC 601 CAACTGCTGG CTGGGCAGGG CCCGCGTCCT CCCCCAGATT CTAGCATGGG 651 TCATCCTGGG CCTCACCTGC TGATGCCAGG GCCATCGTCT TTTCTCAGTC 701 CTTCTCCTTT CCAACCATAC TTGGCTTTGG GGATGACCCC AGACACCCCC 751 TGAATCCAGG TCAGAGGTCA GCCCACCTTT CTTTCTGCTT GCAAAGCCTA 801 TAGACCCTTC TCAGAGCGGT CCTCATGGCT GGGTTTTCTG GGACACATGT 851 CGAGGACAGA AGGTGGAGGG TGGTGGAGCT GCTGCTGGAA GAAGGGGAAG 901 GAAGAGTGGC CCCTCCCCGA GTTCTAAGTC AGGATGAGGC CCACCTGTCC 951 AAGGTATCGG AACCTACCCA GGGGACCCTC AGATCCTCCA CCCACTCCCC 1001 CATCCATTAC GATGCCAGCT TCCAGCCTTG CCCAGGTCAG AGCTGTGGCA 1051 GAGGAGAGGC AGCCAGGCCC TGTTCCTGCT CAGCTCCTGC TCAGGAAGGC 1101 CAGGCCTGAC AGATGTTTGG GAGAGGAATA AAGTTGTGTT GTTGTGGGGC 1151 ATGCAGGCGT GCACACAGCC CTTTTCAAAA AAAAAA BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 3 ORF from 36 bp to 596 bp; peptide length: 187 Category: putative protein 1 MGSPRPPGMR EPPGPSAVMP ALPSTSTCPP RDQGTPEVQP TPAKDTWKGK 51 RPRSQQENPE SQPQKRPRPS AKPSVVAEVK GSVSASEQGT LNPTAQDPFQ 101 LSAPGVSLKE AANVVVKCLT PFYKEGKFAS KELFKGFARH LSHLLTQKTS 151 PGRSVKEEAQ NLIRHFFHGR ARCESEADWH GLCGPQR BLASTP hits Entry MMU92455_1 from database TREMBL: product: "WW domain binding protein 7"; Mus musculus WW domain binding protein 7 mRNA, partial eds. Score = 134, P = 6.9e-08, identities = 45/125, positives = 56/125 Alert BLASTP hits for DKFZphtes3_50j 4, frame 3 No Alert BLASTP hits found Pedant information for DKFZphtes3_50j4, frame 3 Report for DKFZphtes3_50j4.3 [LENGTH] 187 [MW] 20353.06 [pi] 9.76 [PROSITE] MYRISTYL 1 [PROSITE] AMIDATION 1 [PROSITE] CK2_PHOSPHO_SITE [PROSITE] PKC_PHOSPHO_SITE [KW] All_Alpha [KW] LOW COMPLEXITY 1.56 % SEQ MGSPRPPGMREPPGPSAVMPALPSTSTCPPRDQGTPEVQPTPAKDTWKGKRPRSQQENPE SEG xxxxxxxxxxxxxxxx PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ SQPQKRPRPSAKPΞVVAEVKGSVSASEQGTLNPTAQDPFQLSAPGVSLKEAANVVVKCLT SEG PRD cccccccccccccchhhhhccccccccccccccccccccccccccccchhhhhhheeecc SEQ PFYKEGKFASKELFKGFARHLSHLLTQKTSPGRSVKEEAQNLIRHFFHGRARCESEADWH SEG PRD cccccccchhhhhhhhhhhhhhhhheeecccccchhhhhhhhhhhhhhccchhhhhhhhh SEQ GLCGPQR SEG PRD ccccccc Prosite for DKFZphtes3_50j4.3 PS00005 3->6 PKC PHOSPHO SITE PDOC00005 PS00005 46->49 PKC PHOSPHO " " SITE PDOC00005 PS00005 70->73 PKC PHOSPHO " " SITE PDOC00005 PS00005 107-M10 PKC PHOSPHO " " SITE PDOC00005 PS00005 146->149 PKC PHOSPHO " " SITE PDOC00005 PS00005 154->157 PKC PHOSPHO " " SITE PDOC00005 PS00006 54->58 CK2 PHOSPHO " " SITE PDOC00006 PS00006 84->88 CK2 PHOSPHO " " SITE PDOC00006 PS00006 94->98 CK2 PHOSPHO " " SITE PDOC00006 PS00006 107->111 CK2 PHOSPHO " " SITE PDOC00006 PS00006 154->158 CK2 PHOSPHO " " SITE PDOC00006 PS00006 175->179 CK2 PHOSPHO " " SITE PDOC00006 PS00008 81->87 MYRISTYL PDOC00008 PS00009 48->52 AMIDATION PDOC00009 (No Pfam data available for DKFZphtes3_50j 4.3) DKFZphtes3_50n06 group: testes derived DKFZphtes3_50n06 encodes a novel 186 amino acid protein without similarity to known proteins. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application n studying the expression profile of testis-specific genes . unknown complete cDNA, complete eds, EST hits Sequenced by DKFZ Locus : unknown Insert length: 1095 bp Poly A stretch at pos. 1085, polyadenylation signal at pos. 1061 1 CAAGACCCTC GGAGCCAAGA AACAACACTG AGTTCCAGAT TTCGGAAGGT 51 TCACGAGTGT TGCCGACACG CCCTCCCAAC TGCAGACATC CTCCCTGGAG 101 GACCTGCTGT GCTCACATGC CCCCCTGTCC AGCGAGGACG ACACCTCCCC 151 GGGCTGTGCA GCCCCCTCCC AGGCACCCTT CAAGGCCTTC CTCAGTCCCC 201 CAGAGCCACA TAGCCACCGA GGCACCGACA GGAAGCTGTC CCCGCTCCTG 251 AGCCCCTTGC AAGACTCACT GGTGGACAAG ACCCTGCTGG AGCCCAGGGA 301 GATGGTCCGG CCTAAGAAGG TGTGTTTCTC GGAGAGCAGC CTGCCCACCG 351 GGGACAGGAC CAGGAGGAGC TACTACCTCA ATGAGATCCA GAGCTTCGCG 401 GGCGCCGAGA AGGACGCGCG CGTGGTGGGC GAGATCGCCT TCCAGCTGGA 451 CCGCCGCATC CTGGCCTACG TGTTCCCGGG CGTGACGCGG CTCTACGGCT 501 TCACGGTGGC CAACATCCCC GAGAAGATCG AGCAGACCTC CACCAAGTCT 551 CTGGACGGCT CCGTGGACGA GAGGAAGCTG CGCGAGCTGA CGCAGCGCTA 601 CCTGGCCCTG AGCGCGCGCC TGGAGAAGCT GGGCTACAGC CGCGACGTGC 651 ACCCGGCGTT CAGCGAGTTC CTCATCAACA CCTACGGAAT CCTGAAGCAG 701 CGGCCCGACC TGCGCGCCAA CCCCCTGCAC AGCAGCCCGG CCGCGCTGCG 751 CAAGCTGGTC ATCGACGTGG TGCCCCCCAA GTTCCTGGGC GACTCGCTGC 801 TGCTGCTCAA CTGCCTGTGC GAGCTCTCCA AGGAGGACGG CAAGCCCCTC 851 TTCGCCTGGT GAGCCGCCCC GCGCCCGCCG CCTTGCCTGC AGTAAACGCG 901 TTTGTTCCAA CCCGGGGCCG CGGTGCCTCC TGCGCGTCCC CCCGGAGGGG 951 AAAGGGCCGC GTCCCCCGCG CGCGAGGCCA GAGAAGGCCC CGCTCCCACC 1001 GGTGCTGGGC CCCGACCGCA GCCCGCCGCT GCCCGCACCT GCGGAGTGCT 1051 TCTCACCCCT CATTAAAATC ATCCGTTTGC TTGTCAAAAA AAAAA BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 2 ORF from 302 bp to 859 bp; peptide length: 186 Category: putative protein Classification: no clue 1 MVRPKKVCFS ESΞLPTGDRT RRSYYLNEIQ SFAGAEKDAR VVGEIAFQLD 51 RRILAYVFPG VTRLYGFTVA NIPEKIEQTS TKSLDGSVDE RKLRELTQRY 101 LALSARLEKL GYSRDVHPAF SEFLINTYGI LKQRPDLRAN PLHSSPAALR 151 KLVIDVVPPK FLGDSLLLLN CLCELSKEDG KPLFAW BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_50n06, frame 2 No Alert BLASTP hits found Pedant information for DKFZphtes3_50n06, frame 2 Report for DKFZphtes3_50n06.2 [LENGTH] 186 [MW] 21049.39 [pi] 9.28 [KW] All Alpha [KW] LOW COMPLEXITY 5.38 % SEQ MVRPKKVCFSESSLPTGDRTRRSYYLNEIQSFAGAEKDARVVGEIAFQLDRRILAYVFPG SEG PRD ccccceeeccccccccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccc SEQ VTRLYGFTVANIPEKIEQTSTKSLDGSVDERKLRELTQRYLALSARLEKLGYSRDVHPAF SEG PRD ceeeeeeeeeeccccccccccccccccchhhhhhhhhhhhhhhhhhhhhhccccccccch SEQ SEFLINTYGILKQRPDLRANPLHSSPAALRKLVIDVVPPKFLGDSLLLLNCLCELSKEDG SEG xxxxxxxxxx PRD hhhhhhcceeecccccccccccccchhhhhhhhhhccccccccchhhhhhhhhhhhcccc SEQ KPLFAW SEG PRD cccccc (No Prosite data available for DKFZphtes3_50n06.2) (No Pfam data available for DKFZphtes3 50n06.2) DKFZphtes3_50n23 group: testes derived DKFZphtes3_50n23 encodes a novel 499 amino acid protein without similarity to known proteins. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . unknown (from other testis librarys) testis specific cDNA' Sequenced by DKFZ Locus: unknown Insert length: 1907 bp Poly A stretch at pos. 1897, polyadenylation signal at pos. 1872 1 GGGCACCAGC CACTTTCCAC CATGACTGTG CGCTCGAGGG TCGCAGATGT 51 GTTCGGCAGC AAGGACACTG AGAGCCTTGA GCCTGTGCTT TTACCCTTAG 101 TAGATCGCAG GTTTCCTAAG AAATGGGAAA GACCGGTGGC AGAAAGCTTA 151 GGCCACAAAG ACAAAGACCA GGAGGACTAC TTCCAGAAGG GAGGACTCCA 201 AATTAAGTTC CACTGTAGCA AGCAGCTGTC TCTAGAGAGC TCCAGGCAGG 251 TGACCTCTGA GAGCCAAGAG GAGCCCTGGG AGGAGGAATT CGGCCGGGAG 301 ATGCGGAGGC AGCTGTGGCT GGAGGAGGAG GAGATGTGGC AGCAGCGGCA 351 GAAGAAGTGG GCCCTGCTGG AGCAGGAGCA TCAGGAGAAG CTGCGGCAGT 401 GGAATCTGGA AGACCTGGCC AGGGAGCAAC AGCGGAGATG GGTCCAGCTA 451 GAAAAGGAGC AGGAGAGCCC ACGGAGAGAG CCAGAGCAGC TAGGGGAGGA 501 TGTGGAGAGG AGGATCTTCA CACCCACCAG TCGATGGAGG GACTTGGAGA 551 AGGCAGAGCT ATCATTAGTG CCTGCCCCAA GCCGGACCCA ATCTGCTCAC 601 CAAAGCAGGA GGCCACACTT GCCCATGTCT CCTAGTACCC AGCAGCCTGC 651 CCTGGGAAAG CAGAGACCTA TGAGTTCAGT GGAGTTTACC TACAGACCAC 701 GGACCCGCCG AGTTCCCACA AAGCCCAAGA AATCTGCCTC CTTTCCTGTC 751 ACTGGGACAT CCATCCGAAG GCTGACCTGG CCCTCTTTGC AGATATCCCC 801 TGCAAATATT AAGAAGAAGG TGTACCACAT GGACATGGAG GCCCAGAGGA 851 AGAACCTGCA GCTCCTGAGT GAGGAGTCTG AGTTGAGGCT GCCCCACTAC 901 CTGCGCAGCA AAGCACTGGA GCTCACCACC ACCACCATGG AGCTGGGCGC 951 GCTCAGGCTG CAGTACCTGT GCCATAAGTA CATCTTCTAT AGACGCCTCC 1001 AGAGCCTCCG GCAAGAAGCG ATCAACCATG TACAAATCAT GAAAGAAACG 1051 GAGGCTTCCT ACAAGGCCCA GAACCTCTAC ATCTTCCTGG AAAACATTGA 1101 CCGCCTGCAG AGTCTCAGGC TGCAGGCCTG GACGGACAAG CAGAAGGGGC 1151 TGGAGGAGAA GCACCGAGAG TGCCTGAGCA GCATGGTGAC CATGTTCCCC 1201 AAGCTCCAGC TGGAGTGGAA CGTTCACCTG AACATCCCTG AGGTCACCTC 1251 GCCAAAGCCA AAGAAATGCA AGTTGCCTGC AGCCTCACCC CGGCACATCC 1301 GCCCCAGTGG CCCCACCTAC AAGCAGCCCT TTCTGTCTAG GCACCGGGCA 1351 TGTGTGCCCC TGCAGATGGC CCGCCAACAG GGGAAGCAGA TGGAGGCTGT 1401 CTGGAAGACC GAGGTGGCCT CCTCCAGTTA CGCAATAGAA AAAAAGACCC 1451 CTGCCAGCCT TCCCCGGGAC CAGCTGAGGG GACACCCAGA TATTCCCCGG 1501 CTGTTGACAC TGGACGTGTA GTCCTCCTGC CACAAAAGCC TGAACTTCCT 1551 GAAGGCCCAG TAAGCGCCTC AGCGAACCAA AGGAAGGAAT GCCAGGAACC 1601 TACAAATGAA TCCGCTTAGC TTGTTCAAAA AAAGTCAAGC GAGTCACTCC 1651 CTGGAACCCA AATAAGCCAG AAGGATCAAG ACAGCCCCAG TCTCCACTGC 1701 ATCCCTCAGC CAGTGATTCT CAACCTTCTG AGGGACGGAA ACCCACAGAG 1751 AACTTGGTCA AAATGCAGGT TCCCAGCTGG TGCTTTTAAA GAAACCCTCT 1801 GGGGGTTGCT GAGTACTCCT AGAACTTTGA GAAACACTGC TTCCCTCCTG 1851 CAGTCCCCAA ACTCTACATT TTAATAAAAT AGAGGTTGGT TTATTTTAAA 1901 AAAAAAA BLAST Results o BLAST result Medlme entries o Medline entry Peptide information for frame 1 ORF from 22 bp to 1518 bp; peptide length: 499 Category: similarity to known protein Classification: no clue 1 MTVRSRVADV FGSKDTESLE PVLLPLVDRR FPKKWERPVA EΞLGHKDKDQ 51 EDYFQKGGLQ IKFHCSKQLS LESSRQVTSE SQEEPWEEEF GREMRRQLWL 101 EEEEMWQQRQ KKWALLEQEH QEKLRQWNLE DLAREQQRRW VQLEKEQESP 151 RREPEQLGED VERRIFTPTS RWRDLEKAEL SLVPAPSRTQ SAHQSRRPHL 201 PMSPSTQQPA LGKQRPMSSV EFTYRPRTRR VPTKPKKSAS FPVTGTSIRR 251 LTWPSLQISP ANIKKKVYHM DMEAQRKNLQ LLSEESELRL PHYLRSKALE 301 LTTTTMELGA LRLQYLCHKY IFYRRLQSLR QEAINHVQIM KETEASYKAQ 351 NLYIFLENID RLQSLRLQAW TDKQKGLEEK HRECLSSMVT MFPKLQLEWN 401 VHLNIPEVTS PKPKKCKLPA ASPRHIRPSG PTYKQPFLSR HRACVPLQMA 451 RQQGKQMEAV WKTEVASSSY AIEKKTPASL PRDQLRGHPD IPRLLTLDV BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_50n23, frame 1 PIR:S28589 trichohyalm - rabbit, N = 1, Score = 134, P = 5.3e-05 TREMBLNEW:AF132479_1 product: "Ese2L protein"; Mus musculus Ese2L protein mRNA, complete eds., N = 1, Score = 130, P = 0.00017 >PIR:S28589 trichohyalm - rabbit Length = 1,407 HSPs: Score = 134 (20.1 bits), Expect = 5.3e-05, P = 5.3e-05 Identities = 88/354 (24%), Positives = 154/354 (43%) Query: 29 RRFPKKWERPVAESLGHKDKDQEDYFQKGGLQIK-FHCSKQLSLESSRQVTSESQEEPWE 87 R++ K +R + L + ++E ++ G + F +QL +++ E +EE + Sbjct: 165 RQYRDKEQRLQRQELEERRAEEEQLRRRKGRDAEEFIEEEQLRRREQQELKRELREEEQQ 224 Query: 88 EEFGREMRRQLWLEEEEMWQQRQKKWALLEQEHQEKLRQWNLEDLAREQQRRWVQLEKEQ 147 RE + L+EEE RQ++W E Q++LR+ LE++ RE+++R Q E+ + Sbjct: 225 RRERREQHERA-LQEEEEQLLRQRRWRE-EPREQQQLRR-ELEEI-REREQRLEQEERRE 280 Query: 148 ESPRREPEQLGEDVERRIFTPTSRWRDLEKAELSLVPAPSRTQSAHQSRRPHLPMSPSTQ 207 + RRE ++L E ERR ++ + E L R Q Q R + + Sbjct: 281 QQLRRE-QRL-EQEERREQQLRRELEEIREREQRLEQEERREQRLEQEERREQQLKRELE 338 Query: 208 QPALGKQRPMSSVEFTYRPRTRRVPTKPKKSASFPVTGTSIRRLTWPSLQISPANIKK-K 266 + +QR +E R R + + + ++ A G S+ R W S A ++ K Sbjct: 339 EIREREQR LEQEER-REQLLAEEVREQAR—ERGESLTR-RWQRQLESEAGARQΞK 390 Query: 267 VYHMDMEAQRKNLQLLSEESELRLPHYLRSKALELTTTTM ELGALRLQYLCHKY 320 VY +R+ Q L ++ E R R + LE E R Q L + Sbjct: 391 VYS RPRRQEEQSLRQDQERR-QRQERERELEEQARRQQQWQAEEESERRRQRLSARP 446 Query: 321 IFYRRLQSLRQEAINHVQIMKETEASYKAQNLYI-FLENIDRLQΞL-RLQAWTDKQKGLE 378 R Q +E Q +E E + + + FLE ++LQ R Q ++ E Sbjct: 447 SLRER-QLRAEERQEQEQRFREEEEQRRERRQELQFLEEEEQLQRRERAQQLQEEDSFQE 505 Query: 379 EKHR 382 ++ R Sbjct: 506 DRER 509 Score = 119 (17.9 bits), Expect = 2.2e-03, P = 2.2e-03 Identities = 79/357 (22%), Positives = 150/357 (42%) Query: 33 KKWERPVAESLGHKDKDQEDYFQKGGLQIKFHCSKQLSLESSRQVTSESQEEPWEEEFGR 92 ++ E+ + + K +++E Q+ + + +Q R+ + + + EE+F + Sbjct: 990 RREEQELRQERDRKFREEEQLLQE REEERLRRQERDRKFREEERQLRRQELEEQFRQ 1046 Query: 93 EMRRQLWLEEEEMWQQRQKKWALLEQEHQEKLRQWNLEDLAREQQRRWVQLEKEQESPRR 152 E R+ LEE+ + Q++++K L QE K R+ E+ R +Q R QL +E++ R Sbjct: 1047 ERDRKFRLEEQ-IRQEKEEK-QLRRQERDRKFRE EEQQRRRQEREQQLRRERDRKFR 1101 Query: 153 EPEQLGEDVERRIFTPTSRWRDLEKAELSLVPAPSRTQSAHQSR--RPHLPMSPSTQQPA 210 E EQL ++ E R R L + E L+ + + R R + +++ Sb j ct: 1102 EEEQLLQEREEERLRRQERARKLREEE-QLLRREEQLLRQERDRKFREEEQLLQESEEER 1160 Query: 211 LGKQ RPMSSVEFTYRPRTRRVPTKPKKSASFPVTGTSIRRLTWPSLQISPANIKKKV 267 L +Q R + E + R + +++ +R+ Q ++++ Sbjct: 1161 LRRQERERKLREEEQLLQEREEERLRRQERARKLREEEQLLRQEEQELRQERARKLREEE 1220 Query: 268 YHMDMEAQ RKNLQLLS-EESELRLPHYLRSKALELTTTTMELGALRLQYL 316 + E Q R+ QLL EE ELR + + E E LR Q Sbjct: 1221 QLLRQEEQELRQERDRKFREEEQLLRREEQELRRERDRKFREEEQLLQEREEERLRRQER 1280 Query: 317 CHKYIFYRRLQSLRQEAINHVQIMKETEASYKAQNLYIFLENIDRLQ-SLRLQAWTDKQK 375 K + L E ++ +E + Y+A+ + E RL+ LR + +++ Sbjct: 1281 ARK—LREEEEQLLFEEQEEQRLRQERDRRYRAEEQFAREEKSRRLERELRQEEEQRRRR 1338 Query: 376 GLEEKHRE 383 E K RE Sbjct: 1339 ERERKFRE 1346 Score = 109 (16.4 bits), Expect = 1.9e-01, P = 1.7e-01 Identities = 37/113 (32%), Positives = 60/113 (53%) Query: 67 KQLSLESSRQVTSESQ—EEPWEEEFGREMRRQLWLEEEEMWQQRQKKWALLEQEHQEKL 124 +QL E R+ E Q +E EE R+ R + EEE++ Q+R+++ L QE + KL Sbjct: 764 QQLRRERDRKFREEEQLLQEREEERLRRQERERKLREEEQLLQEREEE-RLRRQERERKL 822 Query: 125 RQWNLEDLAREQQRRWVQLEKEQESPRREPEQLGEDVERRIFTPTSRWRDLEKAE 179 R+ E L +E++ ++ +E+E RE EQL E+ + R R L + E Sbjct: 823 REE—EQLLQEREEERLR-RQERERKLREEEQLLRQEEQEL—RQERARKLREEE 872 Score = 107 (16.1 bits), Expect = 3.0e-01, P = 2.6e-01 Identities = 35/109 (32%), Positives = 61/109 (55%) Query: 71 LESSRQVTSESQEEPWE-EEFGREMRRQL WLEEEEMWQQRQKKWALLEQEHQEKLRQ 126 L Q+ ES+EE +E +++RR+ + EEE++ Q+R+++ L QE + KLR+ Sbjct: 742 LREEEQLLQESEEERLRRQEREQQLRRERDRKFREEEQLLQEREEE-RLRRQERERKLRE 800 Query: 127 WNLEDLAREQQRRWVQLEKEQESPRREPEQLGEDVERRIFTPTSRWRDLEKAE 179 E L +E++ ++ +E+E RE EQL ++ E R R L + E Sbjct: 801 E—EQLLQEREEERLR-RQERERKLREEEQLLQEREEERLRRQERERKLREEE 850 Score = 104 (15.6 bits), Expect = 9.4e-02, P = 9.0e-02 Identities = 84/339 (24%), Positives = 149/339 (43%) Query: 67 KQLSLESSRQVTSESQEEPWEEEFGREMRRQL-WLEEEEMWQQRQKKWALLEQE—HQEK 123 +QL E ++ +EE EE RE R++L +LEEEE Q+R++ L E++ +++ Sbjct: 451 RQLRAEERQEQEQRFREE EEQRRERRQELQFLEEEEQLQRRERAQQLQEEDSFQEDR 507 Query: 124 LRQWNLEDLAREQQRRWVQLEKEQESPRR EP EQLGEDVE-RRIFTPTSRWRDL 175 R+ ++ Q RW QL++E + R +P EQL E+ E +R R R+ Sbjct: 508 ERRRRQQEQRPGQTWRW-QLQEEAQRRRHTLYAKPGQQEQLREEEELQREKRRQEREREY 566 Query: 176 EKAELSLVPAPSRTQSAHQSRRPHLPMSPSTQQPALGKQRPMSSVEFTYRPRT RRV 231 + E L + + R + + Q+ L + R + E + R RR Sbjct: 567 REEE-KLQREEDEKRRRQERERQYRELEELRQEEQL-RDRKLREEEQLLQEREEERLRRQ 624 Query: 232 PTKPK KSASFPVTGTSIRRLTWPSLQISPANIKKKVYHMDMEAQRK NLQLLSEE 285 + K + +R+ L+ ++++ + E +RK QLL E Sbjct: 625 ERERKLREEEQLLRQEEQELRQERERKLREEEQLLRREEQELRQERERKLREEEQLLQER 684 Query: 286 SELRLPHYLRSKALE LTTTTMELGALRLQYLCHKYIFYRRL-QSLRQEAINHV— 337 E RL R++ L L EL R + L + RR Q LRQE + Sbjct: 685 EEERLRRQERARKLREEEQLLRQEEQELRQERERKLREEEQLLRREEQLLRQERDRKLRE 744 Query: 338 —QIMKETEASYKAQNLYIFLENIDRLQSLRLQAWTDKQKGLEEKHRECL 385 Q+++E+E + E +L+ R + + ++++ L+E+ E L Sbjct: 745 EEQLLQESEEERLRRQ EREQQLRRERDRKFREEEQLLQEREEERL 789 Score = 103 (15.5 bits), Expect = 1.2e-01, P = 1. le-01 Identities = 42/152 (27%), Positives = 74/152 (48%) Query: 36 ERPVAESLGHKDKDQEDYFQKGGLQIKFHCSKQLSLESSRQVTSESQEEPWEEEFG-REM 94 ER + K +++E ++ +++ +++L E + + E QE E + RE Sbjct: 835 ERLRRQERERKLREEEQLLRQEEQELRQERARKLR-EEEQLLRQEEQELRQERDRKLREE 893 Query: 95 RRQLWLEEEEMWQQRQKKWA LLEQEHQEKLRQWNLEDLAREQQ RRWVQ-LEKE 146 + L EE+E+ Q+R +K LL++ +E+LR+ E RE++ RR Q L +E Sbjct: 894 EQLLRQEEQELRQERDRKLREEEQLLQESEEERLRRQERERKLREEEQLLRREEQELRRE 953 Query: 147 QESPRREPEQLGEDVERRIFTPTSRWRDLEKAE 179 + RE EQL ++ E R R L + E Sbjct: 954 RARKLREEEQLLQEREEERLRRQERARKLREEE 986 Score = 103 (15.5 bits), Expect = 7.8e-01, P = 5.4e-01 Identities = 31/91 (34%), Positives = 52/91 (57%) Query: 67 KQLSLESSRQVTSESQEEPWEEEFGREMRRQLWLEEEEMWQQRQKKWALLEQEHQEKLRQ 126 ++L E R++ E Q EE+ R+ R + EEE++ Q+R+++ L QE KLR+ Sbjct: 642 QELRQERERKLREEEQLLRREEQELRQERERKLREEEQLLQEREEE-RLRRQERARKLRE 700 Query: 127 WNLEDLAREQQRRWVQLEKEQESPRREPEQL 157 E L R++++ +L +E+E RE EQL Sbjct: 701 E--EQLLRQEEQ ELRQERERKLREEEQL 726 Score = 101 (15.2 bits), Expect = 2.0e-01, P = 1.8e-01 Identities = 38/111 (34%), Positives = 57/111 (51%) Query: 72 ESSRQVTSESQEEPWEE-EFGREMRRQLWLEEEEMWQQRQKKWALLEQEHQEKLRQWNLE 130 E R++ E Q EE E RE R+L EEE++ Q+R+++ L QE KLR+ + Sbjct: 931 ERERKLREEEQLLRREEQELRRERARKL-REEEQLLQEREEE-RLRRQERARKLREEE-Q 987 Query: 131 DLAREQQRRWVQLEKEQESPRREPEQLGEDVERRIFTPTSRWRDLEKAELSL 182 L RE+Q +L +E++ RE EQL ++ E R R + E L Sbjct: 988 LLRREEQ ELRQERDRKFREEEQLLQEREEERLRRQERDRKFREEERQL 1035 Score = 101 (15.2 bits), Expect = 1.3e+00, P = 7.2e-01 Identities = 33/108 (30%), Positives = 56/108 (51%) Query: 72 ESSRQVTSEΞQEEPWEEEFGREMRRQLWLEEEEMWQQRQKKWALLEQEHQEKLRQWNLED 131 E R++ E Q EE+ R+ R + EEE++ +Q +++ L QE KLR+ E Sbjct: 841 ERERKLREEEQLLRQEEQELRQERARKLREEEQLLRQEEQE LRQERDRKLREE—EQ 895 Query: 132 LAREQQRRWVQLEKEQESPRREPEQLGEDVERRIFTPTSRWRDLEKAE 179 L R++++ +L +E++ RE EQL ++ E R R L + E Sbjct: 896 LLRQEEQ ELRQERDRKLREEEQLLQESEEERLRRQERERKLREEE 940 Score = 99 (14.9 bits), Expect = 2.0e+00, P = 8.7e-01 Identities = 32/97 (32%), Positives = 50/97 (51%) Query: 72 ESSRQVTSESQEEPWEEEFGREMRRQLWLEEEEMWQQRQKKWALLEQEHQEKLRQWNLED 131 E R+ E Q EE E R L EEE Q +++ L QE + KLR+ E Sbjct i 578 EKRRRQERERQYRELEELRQEEQLRDRKLREEEQLLQEREEERLRRQERERKLREE—EQ 635 Query: 132 LAREQ QRRWVQLEKEQESPRREPEQLGEDVERRI 165 L R++ Q R +L +E++ RRE ++L ++ ER++ Sbjct: 636 LLRQEEQELRQERERKLREEEQLLRREEQELRQERERKL 674 Score = 99 (14.9 bits), Expect = 2.0e+00, P = 8.7e-01 Identities = 34/111 (30%), Positives = 58/111 (52%) Query: 67 KQLSLESSRQVTSESQ—EEPWEEEFGREMRRQLWLEEEEMWQQRQKKWALLEQEHQEKL 124 ++L E R++ E Q +E EE R+ R + EEE++ +Q +++ L QE + KL Sbjct: 664 QELRQERERKLREEEQLLQEREEERLRRQERARKLREEEQLLRQEEQE LRQERERKL 720 Query: 125 RQWNLEDLAREQQRRWVQLEKEQESPRREPEQLGEDVERRIFTPTSRWRDLEK 177 R+ + L RE+Q L +E++ RE EQL ++ E R + L + Sbjct: 721 REEE-QLLRREEQL LRQERDRKLREEEQLLQESEEERLRRQEREQQLRR 768 Score = 98 (14.7 bits), Expect = 2.6e+00, P = 9.2e-01 Identities = 37/146 (25%), Positives = 77/146 (52%) Query: 20 EPVLLPLVDRRFPKKWERPVAESLGHKDKDQEDYFQKGGLQIKFHCSKQLSLESΞRQVTS 79 E LL ++ ++ ER + E + +E+ ++ K +QL + +++ Sbjct: 655 EEQLLRREEQELRQERERKLREEEQLLQEREEERLRRQERARKLREEEQLLRQEEQELRQ 714 Query: 80 ESQEEPWEEEFGREMRRQLWLEEEEMWQQRQKKWALLEQEHQEKLRQWNLED-LAREQQR 138 E + + EEE + +RR+ L +E ++ +++ LL++ +E+LR+ E L RE+ R Sbjct: 715 ERERKLREEE--QLLRREEQLLRQERDRKLREEEQLLQESEEERLRRQEREQQLRRERDR 772 Query: 139 RWVQLEKEQESPRREPEQLG-EDVERRI 165 ++ E+EQ RE E+L ++ ER++ Sbjct: 773 KF—REEEQLLQEREEERLRRQERERKL 798 Score = 97 (14.6 bits), Expect = 3.3e+00, P = 9.6e-01 Identities = 38/129 (29%), Positives = 63/129 (48%) Query: 72 ESSRQVTSESQ—EEPWEEEFGREMRRQLWLEEEEMWQQRQKKWALLEQEHQEKLRQWNL 129 E R++ E Q +E EE R+ R + EEE++ +Q +++ L QE KLR+ Sbjct: 817 ERERKLREEEQLLQEREEERLRRQERERKLREEEQLLRQEEQE LRQERARKLREE— 871 Query: 130 EDLAREQQRRWVQLEKEQESPRREPEQLGEDVERRIFTPTSRWRDLEKAELSLVPAPSRT 189 E L R++++ +L +E++ RE EQL E+ + R R L + E L+ Sbjct: 872 EQLLRQEEQ ELRQERDRKLREEEQLLRQEEQE —RQERDRKLREEE-QLLQESEEE 925 Query: 190 QSAHQSRRPHL 200 + Q R L Sbjct: 926 RLRRQERERKL 936 Score = 96 (14.4 bits), Expect = 4.1e+00, P = 9.8e-01 Identities = 41/132 (31%), Positives = 69/132 (52%) Query: 46 KDKDQEDYFQKGGLQI-KFHCSKQLSLEΞSRQVTSESQEEPWEEEFGREMRRQLWLEEEE 104 +++ QE F + Q+ + ++QL E S Q E + E+ G+ R QL +EE Sbjct: 473 RERRQELQFLEEEEQLQRRERAQQLQEEDSFQEDRERRRRQQEQRPGQTWRWQL QEE 529 Query: 105 MWQQRQKKWALLEQEHQEKLRQWNLEDLAREQQRRWVQLEKEQESPRREPEQLGEDVERR 164 ++R +A Q QE+LR+ E+L RE++R+ E+E+E E Q ED +RR Sbjct: 530 AQRRRHTLYAKPGQ—QEQLREE—EELQREKRRQ EREREYREEEKLQREEDEKRR 581 Query: 165 IFTPTSRWRDLEK 177 ++R+LE+ Sbjct: 582 RQERERQYRELEE 594 Score = 96 (14.4 bits), Expect = 4.1e+00, P = 9.8e-01 Identities = 35/138 (25%), Positives = 76/138 (55%) Query: 28 DRRFPKKWERPVAESL-GHKDKDQEDYFQKGGLQIKFHCSKQLSLESSRQVTSESQEEPW 86 +R++ + E E L K +++E Q+ + ++ L Q+ + ++E Sbjct: 586 ERQYRELEELRQEEQLRDRKLREEEQLLQEREEERLRRQERERKLREEEQLLRQEEQE-L 644 Query: 87 EEEFGREMRRQLWL EEEEMWQQRQKKWALLEQEHQEKLRQWNLEDLAREQQRRWVQL 143 +E R++R + L EE+E+ Q+R++K L +E Q L++ E L R+++ R +L Sbjct: 645 RQERERKLREEEQLLRREEQELRQERERK LREEEQ-LLQEREEERLRRQERAR--KL 698 Query: 144 EKEQESPRREPEQLGEDVERRI 165 +E++ R+E ++L ++ ER++ Sbjct: 699 REEEQLLRQEEQELRQERERKL 720 Score = 95 (14.3 bits), Expect = 5.2e+00, P = 9.9e-01 Identities = 59/282 (20%), Positives = 121/282 (42%) Query: 20 EPVLLPLVDRRFPKKWERPVAESLGHKDKDQEDYFQKGGLQIKFHCSKQLSLESSRQVTS 79 E LL ++ ++ ER + E + +E+ ++ K +QL + +++ Sbjct: 655 EEQLLRREEQELRQERERKLREEEQLLQEREEERLRRQERARKLREEEQLLRQEEQELRQ 714 Query: 80 ESQEEPWEEEFGREMRRQLWLEEEEMWQQRQKKWALLEQEHQEKLRQWNLED-LAREQQR 138 E + + EEE + +RR+ L +E ++ +++ LL++ +E+LR+ E L RE+ R Sbjct: 715 ERERKLREEE—QLLRREEQLLRQERDRKLREEEQLLQESEEERLRRQEREQQLRRERDR 772 Query: 139 RWVQLEKEQESPRREPEQLG-EDVERRIFTPTSRWRDLEKAELSLVPAPSRTQSAHQ—Ξ 195 ++ E+EQ RE E+L ++ ER++ ++ E+ L + + Q Sbjct: 773 KF—REEEQLLQEREEERLRRQERERKLREEEQLLQEREEERLRRQERERKLREEEQLLQ 830 Query: 196 RRPHLPMSPSTQQPALGKQRPMSSVEFTYRPRTRRVPTKPKKSASFPVTGTSIRRLTWPS 255 R + ++ L ++ + E R R ++ +R+ Sbjct: 831 EREEERLRRQERERKLREEEQLLRQE-EQELRQERARKLREEEQLLRQEEQELRQERDRK 889 Query: 256 LQISPANIKKKVYHMDMEAQRK NLQLLΞEESELRLPHYLRSKAL 299 L+ ++++ + E RK QLL E E RL R + L Sbjct: 890 LREEEQLLRQEEQELRQERDRKLREEEQLLQESEEERLRRQERERKL 936 Score = 94 (14.1 bits), Expect = l.le+00, P = 6.8e-01 Identities = 35/116 (30%), Positives = 59/116 (50%) Query: 72 ESSRQVTSESQEEPWEEEFGREMRRQLWLEEEEMWQQRQKKWALLEQEHQEK L 124 E +R++ E Q EE+ R+ R + + EEE++ Q+R+++ L QE K L Sbjct: 977 ERARKLREEEQLLRREEQELRQERDRKFREEEQLLQEREEE-RLRRQERDRKFREEERQL 1035 Query: 125 RQWNLEDLAREQQRRWVQLEKEQESPRREPEQLGEDVERRIFTPTSRWRDLEKAELSL 182 R+ LE+ R+++ R +LE EQ +E +QL R F + R ++ E L Sbjct: 1036 RRQELEEQFRQERDRKFRLE-EQIRQEKEEKQLRRQERDRKFREEEQQRRRQEREQQL 1092 Score = 94 (14.1 bits), Expect = l.le+00, P = 6.8e-01 Identities = 51/166 (30%), Positives = 76/166 (45%) Query: 67 KQLSLESSRQVTSESQ—EEPWEEEFGREMR-RQLWLEEEEMWQQRQKKWALLEQEHQEK 123 ++L E R+ E Q +E EE R+ R R+L EEE++ + Q++ L QE+ Sbjct: 1250 QELRRERDRKFREEEQLLQEREEERLRRQERARKLREEEEQLLFEEQEEQRL RQER 1305 Query: 124 LRQWNLED-LAREQQRRWVQLEKEQESPRREPEQLGEDVERRIFTPTSRWRDLEKAELSL 182 R++ E+ ARE++ R +LE+E R+E EQ R F R E+ E Sbjct: 1306 DRRYRAEEQFAREEKSR—RLEREL RQEEEQRRRRERERKFREEQLRRQQEE-EQRR 1359 Query: 183 VPAPSRTQSAHQSRRPHLPMSPSTQQPALGKQRPMSSVEFTYRPRTRRVP 232 R QSRR L P T+Q A R E+ R++ P Sb j ct: 1360 RQLRERQFREDQSRRQVL--EPGTRQFARVPVRSSPLYEYIQEQRSQYRP 1407 Score = 93 (14.0 bits), Expect = 8.3e+00, P = 1. Oe+00 Identities = 41/145 (28%), Positives = 72/145 (49%) Query: 28 DRRFPKKWERPVAESLGHKDKDQEDYFQKGGLQIKFHCSKQLSLESSRQVTSESQEEPW- 86 +RR ++ ER + E + + Q + + Q + L R + QE+ + Sbjct: 408 ERRQRQERERELEEQARRQQQWQAEEESERRRQ-RLSARPSLRERQLRAEERQEQEQRFR 466 Query: 87 -EEEFGREMRRQL-WLEEEEMWQQRQKKWALLEQE—HQEKLRQWNLEDLAREQQRRWVQ 142 EEE RE R++L +LEEEE Q+R++ L E++ +++ R+ ++ Q RW Q Sbjct: 467 EEEEQRRERRQELQFLEEEEQLQRRERAQQLQEEDSFQEDRERRRRQQEQRPGQTWRW-Q 525 Query: 143 LEKEQESPRR EP EQLGEDVE 162 L++E + R +P EQL E+ E Sbjct: 526 LQEEAQRRRHTLYAKPGQQEQLREEEE 552 Score = 91 (13.7 bits), Expect = 2.4e+00, P = 9. le-01 Identities = 38/110 (34%), Positives = 57/110 (51%) Query: 72 ESSRQVTSESQEEPWEE-EFGREMRRQLWLEEEEMWQQRQKKWALLEQEHQEKLRQWNL- 129 E R++ E Q EE E RE R+L EEE++ Q+R+++ L QE KLR+ Sbjct: 931 ERERKLREEEQLLRREEQELRRERARKL-REEEQLLQEREEE-RLRRQERARKLREEEQL 988 Query: 130 EDLAREQQRRWVQLEKEQESPRREPEQLGEDVERRIFTPTSRWRDLEKAEL 180 ++L +E+ R++ E+EQ RE E+L R F R L + EL Sbjct: 989 LRREEQELRQERDRKF--REEEQLLQEREEERLRRQERDRKFREEER--QLRRQEL 1040 Score = 89 (13.4 bits), Expect = 2.2e+00, P = 8.9e-01 Identities = 35/138 (25%), Positives = 65/138 (47%) Query: 82 QEEPWEEEFGREMRRQLWLEEEEM—WQQRQKKWALLEQEHQEKLRQWNLEDLAREQQRR 139 Q E++ E+R + + +E E WQ+++++ L E+E Q K R+ + +R+ + + Sbjct: 111 QNRRQEDQRRFELRDRQFEDEPERRRWQKQEQERELAEEEEQRKKRERFEQHYSRQYRDK 170 Query: 140 WVQLEKEQ-ESPRREPEQL GEDVERRIFTPTSRWRDLEKAELSLVPAPSRTQSAHQ 194 +L++++ E R E EQL G D E F + R E+ EL Q + Sbjct: 171 EQRLQRQELEERRAEEEQLRRRKGRDAEE--FIEEEQLRRREQQELKR-ELREEEQQRRE 227 Query: 195 SRRPHLPMSPΞTQQPALGKQR 215 R H ++ L ++R Sbjct: 228 RREQHERALQEEEEQLLRQRR 248 Score = 50 (7.5 bits), Expect = 2.2e+00, P = 8.9e-01 Identities = 34/160 (21%), Positives = 67/160 (41%) Query: 325 RLQSLRQEAINHVQIMKETEASYKAQNLYIFLENIDRL-QSLRLQAWTDKQKGLEEKHRE 383 R + R+E Q+ +E E + + LE +R Q LR + ++++ E++ R Sbjct: 245 RQRRWREEPREQQQLRRELEEIREREQR LEQEERREQQLRREQRLEQEERREQQLRR 301 Query: 384 CLSSMVTMFPKLQLEWNVHLNIP-EVTSPKPKKCKLPAASPRHIRPΞGPTYKQPFLSRHR 442 L + +L+ E + E + K +L R R ++ L+ Sbjct: 302 ELEEIREREQRLEQEERREQRLEQEERREQQLKRELEEIREREQRLEQEERREQLLAEEV 361 Query: 443 ACVPLQMARQQGKQMEAVWKTEVASSSYAIEKKTPASLPRDQ 484 + AR++G+ + W+ ++ S + A + K S PR Q Sbjct: 362 R EQARERGESLTRRWQRQLESEAGARQSKV-YΞRPRRQ 398 Score = 40 (6.0 bits), Expect = 1.9e-01, P = 1.7e-01 Identities = 32/115 (27%), Positives = 47/115 (40%) Query: 276 RKNLQLLSEESELRLPHYLRSKAL—ELTTTTMELGALRLQYLCHKYIFYRRL-QSLRQE 332 R+ QLL E E RL R++ L E E LR Q K+ +L Q +E Sbjct: 959 REEEQLLQEREEERLRRQERARKLREEEQLLRREEQELR-QERDRKFREEEQLLQEREEE 1017 Query: 333 AINHVQI MKETEASYKAQNLYI-FLENIDRLQSLRLQAWTDKQ-KGLEEKHRE 383 + + +E E + Q L F + DR L Q +K+ K L + R+ Sbjct: 1018 RLRRQERDRKFREEERQLRRQELEEQFRQERDRKFRLEEQIRQEKEEKQLRRQERD 1073 Score = 37 (5.6 bits), Expect = 1.6e+00, P = 7.9e-01 Identities = 27/108 (25%), Positives = 43/108 (39%) Query: 276 RKNLQLLSEESELRLPHYLRSKAL ELTTTTMELGALRLQYLCHKYIFYRRLQSLRQE 332 R+ QLL E E RL R + L E E LR Q K R + L QE Sbjct: 775 REEEQLLQEREEERLRRQERERKLREEEQLLQEREEERLRRQERERKL REEEQLLQE 831 Query: 333 AINHVQIMKETEASYKAQNLYIFLENIDRLQSLRLQAWTDKQKGLEEKHRE 383 +E E + + + E L+ R + ++++ L ++ +E Sbjct: 832 REEERLRRQERERKLREEEQLLRQEE-QELRQERARKLREEEQLLRQEEQE 881 Pedant information for DKFZphtes3_50n23 , frame 1 Report for DKFZphtes3_50n23. 1 [LENGTH] 499 [MW] 58885 . 69 [pi ] 9 . 67 [KW] All_Alpha [KW] LOW_COMPLEXITY 10.42 % SEQ MTVRSRVADVFGΞKDTESLEPVLLPLVDRRFPKKWERPVAESLGHKDKDQEDYFQKGGLQ SEG PRD ccccccceeecccccccccceeeccccccccccccchhhhhhhcccccccccccccccce SEQ IKFHCSKQLSLESSRQVTSESQEEPWEEEFGREMRRQLWLEEEEMWQQRQKKWALLEQEH SEG xxxxxxxxxx..xxxxxxxxxxxxxxxxxxx PRD eeeecchhhhhhccccccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh SEQ QEKLRQWNLEDLAREQQRRWVQLEKEQESPRREPEQLGEDVERRIFTPTSRWRDLEKAEL SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhccccccccccccccccceeeccccccchhhhhhhh SEQ SLVPAPSRTQSAHQSRRPHLPMSPSTQQPALGKQRPMSSVEFTYRPRTRRVPTKPKKΞAS SEG xxxxxxxxxxxxxxx ... PRD hccccccchhhhhccccccccccccccccccccccccceeeeeeccccccccccccceee SEQ FPVTGTSIRRLTWPSLQISPANIKKKVYHMDMEAQRKNLQLLSEESELRLPHYLRSKALE SEG xxxxxxxx PRD ecccccccccccccccccccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh SEQ LTTTTMELGALRLQYLCHKYIFYRRLQSLRQEAINHVQIMKETEASYKAQNLYIFLENID SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh SEQ RLQSLRLQAWTDKQKGLEEKHRECLSSMVTMFPKLQLEWNVHLNIPEVTSPKPKKCKLPA SEG PRD hhhhhhhhhhhhcchhhhhhhhhhhhhhhhccccchhhhhcccccccccccccccccccc SEQ ASPRHIRPΞGPTYKQPFLSRHRACVPLQMARQQGKQMEAVWKTEVASSSYAIEKKTPASL SEG PRD ccccccccccccccchhhhhhccchhhhhhhhhcchhhhhhhhhhhhhhhhhhhcccccc SEQ PRDQLRGHPDIPRLLTLDV SEG PRD ccccccccccccccccccc (No Prosite data available for DKFZphtes3_50n23.1) (No Pfam data available for DKFZphtes3_50n23.1) DKFZphtes3_6b21 group: testes derived DKFZphtes3_6b21 encodes a novel 781 amino acid protein without similarity to human KIAA0256 gene product. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . similarity to KIAA0256 complete cDNA, complete eds, EST hits Sequenced by BMFZ Locus: /map="356.3 cR from top of Chr9 linkage group" Insert length: 3360 bp Poly A stretch at pos. 3314, polyadenylation signal at pos. 3300 1 GGCAAGCCGA CGGCCCGCTG CTGGCCTCCG TGACGCGGCC TCCTCCGCGC 51 CTCGCGGCAT GGCGTCGGAG GGGCCGCGGG AGCCCGAAAG CGAGGGCATC 101 AAGTTATCAG CAGATGTCAA ACCATTTGTC CCCAGATTTG CCGGGCTCAA 151 TGTGGCATGG TTAGAGTCCT CAGAAGCATG TGTCTTCCCC AGCTCTGCAG 201 CCACATACTA TCCGTTTGTT CAGGAACCAC CAGTGACAGA AATGTTTACT 251 CAGTGCCTGG CTCCCAGTAT CTTTATAACC AACCCAGTTG TTACCGAGGT 301 TTTCAAACAG TGAAGCATCG AAATGAGAAC ACATGCCCTC TCCCACAAGA 351 AATGAAAGCT CTGTTTAAGA AGAAAACCTA TGATGAGAAA AAAACGTATG 401 ATCAGCAAAA GTTTGACAGT GAAAGGGCTG ATGGAACTAT ATCATCTGAG 451 ATAAAATCAG CTAGAGGTTC ACATCATTTG TCCATTTACG CTGAGAATAG 501 TTTGAAATCA GATGGTTACC ATAAGCGAAC AGACAGGAAA TCCAGAATCA 551 TTGCAAAAAA TGTATCTACC TCCAAACCTG AGTTTGAATT TACCACACTG 601 GACTTTCCTG AACTGCAAGG TGCAGAGAAC AATATGTCAG AGATACAGAA 651 GCAACCCAAG TGGGGACCTG TCCACTCTGT CTCTACCGAC ATTTCTCTTC 701 TAAGAGAAGT AGTAAAACCA GCTGCAGTGT TATCAAAGGG TGAAATAGTG 751 GTGAAAAATA ACCCAAATGA ATCTGTAACT GCTAATGCCG CTACCAATTC 801 TCCTTCATGT ACAAGAGAGT TATCTTGGAC ACCAATGGGT TATGTTGTTC 851 GACAGACATT ATCTACAGAA CTGTCAGCAG CCCCTAAAAA TGTTACTTCT 901 ATGATAAACT TAAAGACCAT TGCTTCATCA GCAGATCCTA AAAATGTTAG 951 TATACCATCT TCTGAAGCTT TATCTTCGGA TCCTTCCTAC AACAAAGAAA 1001 AACACATTAT TCATCCTACC CAAAAGTCTA AAGCATCACA AGGTAGTGAC 1051 CTTGAACAAA ATGAAGCCTC AAGAAAGAAT AAGAAAAAGA AAGAAAAATC 1101 TACATCAAAA TATGAAGTCC TGACAGTTCA AGAGCCTCCA AGGATTGAAG 1151 ATGCCGAGGA ATTTCCCAAC CTGGCAGTTG CATCTGAAAG AAGAGACAGA 1201 ATAGAGACAC CGAAATTTCA ATCTAAGCAG CAGCCACAGG ATAATTTTAA 1251 AAATAATGTA AAGAAGAGCC AGCTTCCAGT GCAGTTGGAC TTGGGGGGCA 1301 TGCTGACAGC CCTGGAGAAG AAGCAGCACT CTCAGCATGC AAAGCAGTCC 1351 TCCAAACCAG TGGTAGTCTC AGTTGGAGCA GTGCCAGTCC TTTCCAAAGA 1401 ATGTGCATCA GGGGAGAGAG GCCGCCGCAT GAGTCAAATG AAGACCCCGC 1451 ACAATCCCTT GGACTCCAGC GCCCCACTGA TGAAGAAAGG GAAGCAGAGG 1501 GAGATCCCCA AGGCCAAGAA GCCAACCTCA CTGAAGAAGA TTATTTTGAA 1551 AGAACGGCAA GAGAGAAAGC AGCGTCTCCA AGAAAATGCT GTGAGTCCAG 1601 CTTTTACCAG TGATGACACA CAAGATGGAG AGAGTGGTGG TGATGACCAG 1651 TTTCCCGAGC AGGCAGAGCT GTCAGGGCCA GAGGGGATGG ACGAACTGAT 1701 CTCCACTCCT TCGGTTGAGG ACAAGTCTGA AGAGCCACCA GGCACAGAGC 1751 TCCAGAGGGA CACAGAGGCC TCCCACCTTG CTCCCAATCA CACCACCTTC 1801 CCTAAGATCC ACAGCCGCAG ATTCAGGGAT TACTGCAGCC AGATGCTTAG 1851 TAAAGAAGTG GATGCTTGTG TTACCGACCT ACTCAAAGAA CTGGTCCGTT 1901 TCCAAGACCG TATGTACCAG AAAGATCCAG TCAAGGCCAA GACTAAACGT 1951 CGACTTGTGT TGGGGTTGAG GGAGGTTCTC AAACACCTGA AGCTCAAAAA 2001 ACTGAAATGT GTCATTATTT CTCCCAACTG TGAGAAGATA CAGTCAAAAG 2051 GTGGGCTGGA TGACACTTTG CACACAATTA TTGATTATGC CTGTGAGCAG 2101 AACATTCCCT TTGTGTTTGC TCTCAACCGC AAAGCTCTGG GGCGCAGTTT 2151 GAATAAGGCA GTTCCTGTCA GTGTGGTGGG GATCTTCAGC TATGATGGGG 2201 CCCAGGATCA GTTCCACAAG ATGGTTGAGC TGACAGTGGC GGCCCGACAG 2251 GCGTACAAGA CCATGCTGGA GAATGTGCAG CAGGAGCTGG TGGGAGAGCC 2301 CAGGCCTCAG GCACCTCCCA GCCTACCCAC ACAGGGCCCC AGCTGCCCTG 2351 CAGAAGATGG CCCCCCAGCC CTGAAAGAAA AAGAAGAGCC ACACTACATT 2401 GAAATCTGGA AAAAACATCT GGAAGCATAC AGTGGATGTA CCCTGGAGCT 2451 AGAAGAATCC TTGGAGGCTT CAACCTCTCA AATGATGAAT TTGAATTTAT 2501 GAGAGTTCTT GCCTGTGTGT CTGTATTTTG GGTAAGGAGG GGAGGTCTGA 2551 AAAAGACTTT GGGGCTTTTT CTTCTGTTTT TCATGACAAT GTAATTTGTG 2601 TAACTGTTGA ATCTGGAAAT TGATCAGCAT TAAAGGGCAC ATGAAGCAGT 2651 GTCTGCAGGC GTTCAGTGCT GCGGAGCCTG TTAAAGGTCA CTCAGATGTG 2701 CAGGTGTTAA TCTTCTCTAA AAGCCTGGTT ATACAGCTCT GGCTTTCTGA 2751 GCACACTACG GATCTGGAAA ATACTGGAAA ATGTGATACT TAGAATACTT 2801 TGGCTGCTAA GGAAACTTCC TCTCCATTGC AGAATAGCTG AGCCAAGTGA 2851 GTGAGTTTGC AGAAAGCAGG TGGTGAGCTC CTGCCTGCTG GAGGTTGCCA 2901 TGGAGGGCCA TTCCTGCCCG GCAACAGCAC CGTCCTGCAG GGAGCCACTT 2951 GGCAGAAGGG TGCAGGGCTG CTGGTGTCAG AGCAAGAGGG CTACAGGGAA 3001 AGGGCCCTTT CTCAGGGGAT GTAGCTTTTT TAAAAGATTT GGGAACACTT 3051 GGAGGATTTG CTAAAATGAG CCTCAGAAGG AAAATTGGTT TTCTAACCTG 3101 TGACTTTTTG AAATGAATTA TTCCTTTCAG TCTTTATTTT TCAAAGAAAC 3151 AATGTGTATT GAAGTACCTA GATTTGTTTG ATAATCAACA AATCTTTCCT 3201 TTTTCAATGA ACATATTCTG AATGTGGTTT CTGTCTTAGA CCAGGAGGAC 3251 AGAGTTTGCT TTCATATTTT CCCTGTAAGT AAGAGGGCTT ATTTATTTTA 3301 AATAAAGAGT AATTATTAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 3351 AAAAAAAAAA BLAST Results Entry HS773347 from database EMBL: human STS WI-18160. Score = 813, P = 2.9e-30, identities = 167/171 Medlme entries No Medline entry Peptide information for frame 1 ORF from 157 bp to 2499 bp; peptide length: 781 Category: similarity to known protein 1 MVRVLRSMCL PQLCSHILSV CSGTTSDRNV YΞVPGSQYLY NQPSCYRGFQ 51 TVKHRNENTC PLPQEMKALF KKKTYDEKKT YDQQKFDSER ADGTISSEIK 101 SARGSHHLΞI YAENSLKSDG YHKRTDRKSR IIAKNVSTSK PEFEFTTLDF 151 PELQGAENNM SEIQKQPKWG PVHSVSTDIS LLRE VKPAA VLSKGEIVVK 201 NNPNESVTAN AATNSPSCTR ELSWTPMGYV VRQTLΞTELS AAPKNVTSMI 251 NLKTIASSAD PKNVSIPSSE ALSΞDPSYNK EKHIIHPTQK SKASQGSDLE 301 QNEASRKNKK KKEKSTSKYE VLTVQEPPRI EDAEEFPNLA VASERRDRIE 351 TPKFQSKQQP QDNFKNNVKK SQLPVQLDLG GMLTALEKKQ HSQHAKQSSK 401 PVVVSVGAVP VLSKECASGE RGRRMSQMKT PHNPLDSSAP LMKKGKQREI 451 PKAKKPTSLK KIILKERQER KQRLQENAVS PAFTSDDTQD GESGGDDQFP 501 EQAELSGPEG MDELISTPSV EDKSEEPPGT ELQRDTEASH LAPNHTTFPK 551 IHSRRFRDYC SQMLSKEVDA CVTDLLKELV RFQDRMYQKD PVKAKTKRRL 601 VLGLREVLKH LKLKKLKCVI ISPNCEKIQS KGGLDDTLHT IIDYACEQNI 651 PFVFALNRKA LGRSLNKAVP VSVVGIFSYD GAQDQFHKMV ELTVAARQAY 701 KTMLENVQQE LVGEPRPQAP PSLPTQGPSC PAEDGPPALK EKEEPHYIEI 751 WKKHLEAYSG CTLELEESLE ASTSQMMNLN L BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_6b21, frame 1 SWISSPROT :Y256_HUMAN HYPOTHETICAL PROTEIN KIAA0256., N = 1, Score = 786, P = 3.6e-78 TREMBL :PFMAL3P3_15 gene: "MAL3P3.15"; Plasmodium falciparum MAL3P3, N = 2, Score = 161, P = 5. le-10 TREMBL :RNNFLH_1 Rat heavy neurofilament subunit (NF-H) mRNA, 3' end., N = 1, Score = 150, P = 9. le-07 >SWISSPROT:Y256_HUMAN HYPOTHETICAL PROTEIN KIAA0256. Length = 635 HSPs: Score = 786 (117.9 bits), Expect = 3.6e-78, P = 3.6e-78 Identities = 190/424 (44%), Positives = 263/424 (62%) Query: 369 KKSQLPVQLDLGGMLTALEKKQHΞQHAKQ—SSKPVVVSVGAVPVLSKECASGERGRRMS 426 KK++ PVQLDLG ML ALEK+Q + A+Q +++P+ +V + ++ + S Sbjct: 16 KKNKTPVQLDLGDMLAALEKQQQAMKARQITNTRPLSYTVVTAASFHTKDSTNRKPLTKS 75 Query: 427 Q-MKTPHNPLDSSAPLMKKGKQREIPKAKKPTSLKKIILKERQERKQRLQENAVSPAFTS 485 Q T N +D ++ KKGK++EI K K+PT+LKK+ILKER+E+K RL + S Sbjct: 76 QPCLTSFNSVDIASSKAKKGKEKEIAKLKRPTALKKVILKEREEKKGRLTVD—HNLLGS 133 Query: 486 DDTQDGEΞGGDDQFPEQAELSGPEGMDELISTPSVEDKSEEPPG—TELQRDTEASHL— 541 ++ + D P++ G+ + S S+ S+ P T + + + AS Sbjct: 134 EEPTEMHLDFIDDLPQEIVSQEDTGLS-MPSDTSLSPASQNSPYCMTPVSQGSPASSGIG 192 Query: 542 APN-HTTFPKIHSRRFRDYCSQMLSKEVDACVTDLLKELVRFQDRMYQKDPVKAKTKRRL 600 +P +T KIHS+RFR+YC+Q+L KE+D CVT LL+ELV FQ+R+YQKDPV+AK +RRL Sbjct: 193 SPMASSTITKIHSKRFREYCNQVLCKEIDECVTLLLQELVSFQERIYQKDPVRAKARRRL 252 Query: 601 VLGLREVLKHLKLKKLKCVIISPNCEKIQSKGGLDDTLHTIIDYACEQNIPFVFALNRKA 660 V+GLREV KH+KL K+KCVIISPNCEKIQSKGGLD+ L+ +1 A EQ IPFVFAL RKA Sbjct: 253 VMGLREVTKHMKLNKIKCVIISPNCEKIQSKGGLDEALYNVIAMAREQEIPFVFALGRKA 312 Query: 661 LGRSLNKAVPVSVVGIFSYDGAQDQFHKMVELTVAARQAYKTMLENVQQELVGEPRP 717 LGR +NK VPVSVVGIF+Y GA+ F+K+VELT AR+AYK M+ ++QE E Sbjct: 313 LGRCVNKLVPVSVVGIFNYFGAESLFNKLVELTEEARKAYKDMVAAMEQEQAEEALKNVK 372 Query: 718 QAPPSLP-TQGPS CPAEDGPPALKEKEEPHYIEIWKKHLEAYSGCTL ELE 766 + P + ++ PS C P + E E Y W+ +E G E E Sbjct: 373 KVPHHMGHSRNPSAASAISFCSVISEP—IΞEVNEKEYETNWRNMVETSDGLEAΞENEKE 430 Query: 767 ESLEASTSQ 775 S + STS+ Sbjct: 431 VSCKHSTSE 439 Pedant information for DKFZphtes3_6b21, frame 1 Report for DKFZphtes3_6b21.1 [LENGTH] 781 [MW] 87393.44 [pi] 8.94 [HOMOL] SWISSPROT :Y256_HUMAN HYPOTHETICAL PROTEIN KIAA0256. 4e-75 [PROSITE] MYRISTYL 4 [PROSITE] AMIDATION 1 [PROSITE] CAMP_PHOSPHO_SITE 3 [PROSITE] CK2_PHOSPHO_SITE 16 [PROSITE] TYR_PHOSPHO_SITE 4 [PROSITE] PKC_PHOSPHO_SITE 16 [PROSITE] ASN_GLYCOSYLATION 6 [KW] Alpha_Beta [KW] LOW COMPLEXITY 8.45 % SEQ MVRVLRSMCLPQLCSHILSVCΞGTTSDRNVYSVPGSQYLYNQPSCYRGFQTVKHRNENTC SEG PRD ccceeeeeccceeeeeeeeeeccccccccccccccccccccccceeeceeeeeecccccc SEQ PLPQEMKALFKKKTYDEKKTYDQQKFDSERADGTISSEIKSARGSHHLSIYAENSLKSDG SEG xxxxxxxxxxxx PRD cccchhhhhhhhhhccchhhhhhhhhhhccccccchhhhhhhcccceeeeeeeecccccc SEQ YHKRTDRKSRIIAKNVSTSKPEFEFTTLDFPELQGAENNMSEIQKQPKWGPVHSVSTDIS SEG PRD cccccchhhhheeeccccccccceeecccccccccccchhhhhhccccccccceeecchh SEQ LLREVVKPAAVLSKGEIVVKNNPNESVTANAATNSPSCTRELSWTPMGYVVRQTLΞTELS SEG PRD hhhhhhheeeeecccceeeeccccceeeeeecccccccceeeeeccceeeeeeccccccc SEQ AAPKNVTSMINLKTIASSADPKNVSIPSSEALSSDPSYNKEKHIIHPTQKSKASQGSDLE SEG PRD ccccceeeeehhhhhhcccccceeeecccccccccccccccceeechhhhhhhcccccch SEQ QNEAΞRKNKKKKEKSTSKYEVLTVQEPPRIEDAEEFPNLAVASERRDRIETPKFQSKQQP SEG .... xxxxxxxxxxxxxx PRD hhhhccccccccccccceeeeeecccccchhhhhhccchhhhhhhhhhhhcccccccccc SEQ QDNFKNNVKKSQLPVQLDLGGMLTALEKKQHSQHAKQSSKPVVVSVGAVPVLSKECASGE SEG xxxxxxxxxxxxxxxxx PRD cccccccccccccccccccchhhhhhhhhhhhhhhhhhhccceeeeeeeeeeeecccccc PS00008 633->639 MYRISTYL PDOC00008 PS00009 421->425 AMIDATION PDOC00009 (No Pfam data available for DKFZphtes3_6b21.1) DKFZphtes3_6cll group: signal transduction DKFZphtes3_6cll encodes a novel 1025 amino acid protein with similarity to A. ambisexualis antheridiol steroid receptor. The novel protein is a putative steroid receptor. It shares similarity with yeast YNL132w and contains the ATP/GTP-bindmg site motif A (P-loop) and RGD site, similar to the A. ambisexualis antheridiol steroid receptor. The new protein can find application in modulating/blockmg the expression of genes controlled by this receptor. strong similarity to YNL132w strong similarity to S. pombe/YDK9_SCHPO, S . cerevιsιae/YNL132w, C.elegans/F55A12.8 Sequenced by BMFZ Locus : unknown Insert length: 3966 bp Poly A stretch at pos. 3890, polyadenylation signal at pos. 3873 1 GCTGTGCCTT CTCTTTCGGA GTTGTTCCGT GCTCCCACGT GCTTCCCCTT 51 CTCCACTGGC TGGGATCCCC CGGGCTCGGG GCGCAGTAAT AATTTTTCAC 101 CATGCATCGG AAAAAGGTGG ATAACCGAAT CCGGATTCTC ATTGAGAATG 151 GAGTAGCTGA GCGGCAAAGA TCTCTCTTTG TTGTAGTTGG GGATCGAGGA 201 AAAGATCAGG TGGTAATACT TCATCACATG TTATCCAAAG CAACTGTGAA 251 GGCTCGGCCT TCAGTGCTGT GGTGTTATAA GAAAGAGCTG GGGTTTAGCA 301 GTCACCGGAA GAAAAGAATG CGACAGCTGC AGAAGAAAAT AAAGAATGGA 351 ACACTGAACA TAAAGCAGGA CGACCCCTTT GAACTCTTCA TAGCAGCCAC 401 AAACATTCGC TACTGCTACT ACAACGAGAC CCACAAGATC CTGGGCAATA 451 CCTTCGGCAT GTGTGTGCTG CAGGATTTTG AAGCCTTAAC TCCAAACTTG 501 CTGGCCAGGA CTGTAGAAAC AGTGGAAGGT GGTGGGCTAG TGGTCATCCT 551 CCTACGGACC ATGAACTCAC TCAAGCAATT GTACACAGTG ACTATGGATG 601 TGCATTCCAG GTACAGAACT GAGGCCCATC AGGATGTGGT GGGAAGATTT 651 AATGAAAGGT TTATTCTGTC TCTGGCCTCT TGTAAGAAGT GTCTCGTCAT 701 TGATGACCAG CTCAACATCC TGCCCATCTC CTCCCACGTT GCCACCATGG 751 AGGCCCTGCC TCCCCAGACT CCGGATGAGA GTCTTGGTCC TTCTGATCTG 801 GAGCTGAGGG AGTTGAAGGA GAGCTTGCAG GACACCCAGC CTGTGGGTGT 851 GTTGGTGGAC TGCTGTAAGA CTCTAGACCA GGCCAAAGCT GTCTTGAAAT 901 TTATCGAGGG CATCTCTGAA AAGACCCTGA GGAGTACTGT TGCACTCACA 951 GCTGCTCGAG GACGGGGAAA ATCTGCAGCC CTGGGATTGG CGATTGCTGG 1001 GGCGGTGGCA TTTGGGTACT CCAATATCTT TGTTACCTCC CCAAGCCCTG 1051 ATAACCTCCA TACTCTGTTT GAATTTGTAT TTAAAGGATT TGATGCTCTG 1101 CAATATCAGG AACATCTGGA TTATGAGATT ATCCAGTCTC TAAATCCTGA 1151 ATTTAACAAA GCAGTGATCA GAGTGAATGT ATTTCGAGAA CACAGGCAGA 1201 CTATTCAGTA TATACATCCT GCAGATGCTG TGAAGCTGGG CCAGGCTGAA 1251 CTAGTTGTGA TTGATGAAGC TGCCGCCATC CCCCTCCCCT TGGTGAAGAG 1301 CCTACTTGGC CCCTACCTTG TTTTCATGGC ATCCACCATC AATGGCTATG 1351 AGGGCACTGG CCGGTCACTG TCCCTCAAGC TAATTCAGCA GCTCCGTCAA 1401 CAGAGCGCCC AGAGCCAGGT CAGCACCACT GCTGAGAATA AGACCACGAC 1451 GACAGCCAGA TTGGCATCAG CGCGGACACT GCATGAGGTT TCCCTCCAGG 1501 AGTCAATCCG ATACGCCCCT GGGGATGCAG TGGAGAAGTG GCTGAATGAC 1551 TTGCTGTGCC TGGATTGCCT CAACATCACT CGGATAGTCT CAGGCTGCCC 1601 CTTGCCTGAA GCTTGTGAAC TGTACTATGT TAATAGAGAT ACCCTCTTTT 1651 GCTACCACAA GGCCTCTGAA GTTTTCCTCC AACGGCTTAT GGCCCTCTAC 1701 GTGGCTTCTC ACTACAAGAA CTCTCCCAAT GATCTCCAGA TGCTCTCCGA 1751 TGCACCTGCT CACCATCTCT TCTGCCTTCT GCCTCCTGTG CCCCCCACCC 1801 AGAATGCCCT TCCAGAAGTG CTTGCTGTTA TCCAGGTGTG CCTTGAAGGG 1851 GAGATTTCTC GCCAGTCCAT CTTGAACAGT CTGTCTCGAG GCAAGAAGGC 1901 TTCAGGGGAC CTGATTCCAT GGACAGTGTC AGAACAGTTC CAAGATCCAG 1951 ACTTTGGTGG TCTGTCTGGT GGAAGGGTCG TTCGCATTGC TGTTCACCCA 2001 GATTATCAAG GGATGGGCTA TGGCAGCCGT GCTCTGCAGC TGCTGCAGAT 2051 GTACTATGAA GGCAGGTTTC CTTGTCTGGA GGAAAAGGTC CTTGAGACAC 2101 CACAGGAAAT TCACACCGTA AGCAGCGAGG CTGTCAGCTT GTTGGAAGAG 2151 GTCATCACTC CCCGGAAGGA CCTGCCTCCT TTACTCCTCA AATTGAATGA 2201 GAGGCCTGCC GAACGCCTGG ATTACCTGGG TGTTTCCTAT GGCTTGACCC 2251 CCAGGCTCCT CAAGTTCTGG AAACGAGCTG GATTTGTTCC TGTTTATCTG 2301 AGACAGACCC CGAATGACCT GACCGGAGAG CACTCGTGCA TCATGCTGAA 2351 GACGCTCACT GATGAGGATG AGGCTGACCA GGGAGGCTGG CTTGCAGCCT 2401 TCTGGAAAGA TTTCCGACGG CGGTTCCTAG CCTTGCTCTC CTACCAGTTC 2451 AGTACCTTCT CTCCTTCCCT GGCTCTGAAC ATCATTCAGA ACAGGAACAT 2501 GGGGAAGCCA GCCCAGCCTG CCCTGAGCCG GGAGGAGCTG GAAGCACTCT 2551 TCCTCCCCTA TGACCTGAAG CGGCTGGAGA TGTATTCACG GAATATGGTG 2601 GACTATCACC TCATCATGGA CATGATCCCG GCCATCTCTC GCATCTATTT 2651 CCTGAACCAG CTGGGGGACC TGGCCCTGTC TGCGGCTCAG TCGGCTCTTC 2701 TCTTGGGGAT TGGCCTGCAG CATAAGTCTG TGGACCAGCT GGAAAAGGAG 2751 ATTGAGCTGC CCTCGGGCCA GTTGATGGGA CTTTTCAACC GGATCATCCG 2801 CAAAGTTGTG AAGCTATTTA ATGAAGTTCA GGAAAAGGCC ATTGAGGAGC 2851 AGATGGTGGC AGCGAAGGAT GTGGTCATGG AGCCCACGAT GAAGACCCTC 2901 AGTGACGACC TAGATGAAGC AGCAAAGGAA TTTCAGGAGA AACACAAGAA 2951 GGAAGTAGGG AAGCTGAAGA GCATGGACCT CTCTGAATAC ATAATCCGTG 3001 GGGACGATGA AGAGTGGAAT GAAGTTTTGA ACAAAGCTGG GCCGAACGCC 3051 TCGATCATCA GCCTGAAAAG TGACAAGAAA AGGAAGTTAG AGGCCAAACA 3101 AGAACCCAAA CAGAGCAAGA AGTTGAAGAA CAGAGAGACA AAGAACAAAA 3151 AAGATATGAA ACTGAAGCGG AAGAAATAGT GAAGAGAAAC TCGGGCATCT 3201 GTGTTTGATC ATGGGAAGAT ACTCTCACTA ACTGAACCCT CTCTGGCTGG 3251 ACTGTTAAAA GCAACGAGAG GCCCCGGCAC ACCTGGAAGC TGGCCGCGAA 3301 TTCGGCCTCT GGGCCTGTGT GTCTGTGAGC TCAACCTGGC TAAAGGCAGA 3351 GTCACTCCCA AATGGGTCTC TTTAGAACTT GATGGCTGGG CACTGCCATC 3401 TCTAGAATTG CCACGAGTCT CTCTCTTCCT GCCCAGTCCA GGGCCCTCCT 3451 TTCCTATAAG TTCATATTTT GCTTTGAGCC AGCTTTTTAG TCTCATTCCC 3501 ACACATGTGG AAGCCACGTT GCCTCTCGAC CGCCTGAGGC CCTTAAGTAC 3551 ATCGCTTTCT GGTGGTGCCC AGGAGGCTGC TGCTGGGCCG CTGGGTCTCT 3601 CTTTGTGGAC TTGTACCTGG AGCAGGAGGA ACTCCAGTCC GTCCCGGCAT 3651 CCATGGCAGC CCGCGGTTAG GTGCGCCAGG GTTTGCTGAT GTTGTCTTGT 3701 GCTGTTCCAC TCTTGGCTCC AGCAGACCCA CTGTCCCAGA AAAGCCTGAT 3751 CCTGTAGTTT ATGTAGAATG CCACATCTGC GTCCTCAAGA CCTGTTTCAT 3801 CCATTTGGGA AAAGATGTTG GGAAAGGCCA CTTTGCTCGC AGGGGTGAGG 3851 GGAAGGATAG AGAATCTATT TTTAATAAAT AACATTCTAG AATGAAAAAA 3901 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 3951 AAAAAAAAAA AAAAAA BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 3 ORF from 102 bp to 3176 bp; peptide length: 1025 Category: similarity to unknown protein Classification: unclassified Prosite motifs: RGD (966-969) ATP GTP A (284-292) 1 MHRKKVDNRI RILIENGVAE RQRSLFVVVG DRGKDQVVIL HHMLSKATVK 51 ARPSVLWCYK KELGFSSHRK KRMRQLQKKI KNGTLNIKQD DPFELFIAAT 101 NIRYCYYNET HKILGNTFGM CVLQDFEALT PNLLARTVET VEGGGLVVIL 151 LRTMNSLKQL YTVTMDVHSR YRTEAHQDVV GRFNERFILS LASCKKCLVI 201 DDQLNILPIS SHVATMEALP PQTPDESLGP SDLELRELKE SLQDTQPVGV 251 LVDCCKTLDQ AKAVLKFIEG ISEKTLRSTV ALTAARGRGK SAALGLAIAG 301 AVAFGYSNIF VTSPSPDNLH TLFEFVFKGF DALQYQEHLD YEIIQSLNPE 351 FNKAVIRVNV FREHRQTIQY IHPADAVKLG QAELVVIDEA AAIPLPLVKS 401 LLGPYLVFMA STINGYEGTG RSLSLKLIQQ LRQQSAQSQV STTAENKTTT 451 TARLASARTL HEVSLQESIR YAPGDAVEKW LNDLLCLDCL NITRIVSGCP 501 LPEACELYYV NRDTLFCYHK ASEVFLQRLM ALYVASHYKN SPNDLQMLSD 551 APAHHLFCLL PPVPPTQNAL PEVLAVIQVC LEGEISRQSI LNSLSRGKKA 601 SGDLIPWTVS EQFQDPDFGG LSGGRVVRIA VHPDYQGMGY GSRALQLLQM 651 YYEGRFPCLE EKVLETPQEI HTVSSEAVSL LEEVITPRKD LPPLLLKLNE 701 RPAERLDYLG VSYGLTPRLL KFWKRAGFVP VYLRQTPNDL TGEHSCIMLK 751 TLTDEDEADQ GGWLAAFWKD FRRRFLALLS YQFSTFSPSL ALNIIQNRNM 801 GKPAQPALSR EELEALFLPY DLKRLEMYSR NMVDYHLIMD MIPAISRIYF 851 LNQLGDLALS AAQSALLLGI GLQHKSVDQL EKEIELPSGQ LMGLFNRIIR 901 KVVKLFNEVQ EKAIEEQMVA AKDVVMEPTM KTLSDDLDEA AKEFQEKHKK 951 EVGKLKSMDL SEYIIRGDDE EWNEVLNKAG PNASIISLKS DKKRKLEAKQ 1001 EPKQSKKLKN RETKNKKDMK LKRKK BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_6cll, frame 3 TREMBL:CEAF3130_4 gene: "F55A12.8"; Caenorhabditis elegans cosmid F55A12., N = 1, Score = 2782, P = l.le-289 PIR:S55151 probable membrane protein YNL132w - yeast (Saccharomyces cerevisiae), N = 2, Score = 2549, P = 3.5e-273 SWISSPROT :YXX1_ACHAM HYPOTHETICAL PROTEIN (FRAGMENT)., N = 1, Score = 1013, P = 3.2e-102 SWISSPRO :YDK9_SCHPO HYPOTHETICAL 116.5 KD PROTEIN C20G8.09C IN CHROMOSOME I., N = 1, Score = 2843, P = 3.8e-296 >SWISΞPROT:YDK9_SCHPO HYPOTHETICAL 116.5 KD PROTEIN C20G8.09C IN CHROMOSOME I. Length = 1,033 HSPs: Score = 2843 (426.6 bits), Expect = 3.8e-296, P = 3.8e-296 Identities = 576/1033 (55%), Positives = 750/1033 (72%) Query: 1 MHRKKVDNRIRILIENGVAERQRSLFVVVGDRGKDQVVILHHMLSKATVKARPSVLWCYK 60 M +K +D+RI LI+NG -E+QRS FVVVGDR +DQVV LH +LS++ V ARP+VLW YK Sbjct: 1 MPKKALDSRIPTLIKNGCQEKQRSFFVVVGDRARDQVVNLHWLLSQSKVAARPNVLWMYK 60 Query: 61 KEL-GFSSHRKKRMRQLQKKIKNGTLNIKQDDPFELFIAATNIRYCYYNETHKILGNTFG 119 K+L GF+SHRKKR +++K+IK G + +DPFELF + TNIRYCYY E+ KILG T+G Sbjct: 61 KDLLGFTSHRKKRENKIKKEIKRGIRDPNSEDPFELFCSITNIRYCYYKESEKILGQTYG 120 Query: 120 MCVLQDFEALTPNLLARTVETVEGGGLVVILLRTMNSLKQLYTVTMDVHSRYRTEAHQDV 179 M VLQDFEALTPNLLART+ETVEGGG+VV+LL +NSLKQLYT++MD+HSRYRTEAH DV Sbjct: 121 MLVLQDFEALTPNLLARTIETVEGGGIVVLLLHKLNSLKQLYTMSMDIHSRYRTEAHSDV 180 Query: 180 VGRFNERFILSLASCKKCLVIDDQLNILPISSHVATMEALPPQTPDESLGPSDLELRELK 239 RFNERFILSL +C+ CLVIDD+LN+LPIS ++ALPP +++ + ++EL+ Sbjct: 181 TARFNERFILSLGNCENCLVIDDELNVLPISGG-KNVKALPPTLEEDN—STQNSIKELQ 237 Query: 240 ESLQDTQPVGVLVDCCKTLDQAKAVLKFIEGISEKTLRSTVALTAARGRGKSAALGLAIA 299 ESL + P G LV KTLDQA+AVL F+E I EK+L+ TV+LTA RGRGKSAALGLAIA Sbjct: 238 ESLGEDHPAGALVGVTKTLDQARAVLTFVESIVEKSLKGTVSLTAGRGRGKSAALGLAIA 297 Query: 300 GAVAFGYSNIFVTSPSPDNLHTLFEFVFKGFDALQYQEHLDYEIIQSLNPEFNKAVIRVN 359 A+A GYSNIF+TSPSP+NL TLFEF+FKGFDAL Y+EH+DY+IIQS NP ++ A++RVN Sbjct: 298 AAIAHGYSNIFITSPSPENLKTLFEFIFKGFDALNYEEHVDYDIIQSTNPAYHNAIVRVN 357 Query: 360 VFREHRQTIQYIHPADAVKLGQAELVVIDEAAAIPLPLVKSLLGPYLVFMASTINGYEGT 419 +FR+HRQTIQYI P D+ LGQAELVVIDEAAAIPLPLV+ L+GPYLVFMASTINGYEGT Sbjct: 358 IFRDHRQTIQYISPEDSNVLGQAELVVIDEAAAIPLPLVRKLIGPYLVFMASTINGYEGT 417 Query: 420 GRSLSLKLIQQLRQQSAQSQVSTTAENKTTTTARLASARTLHEVSLQESIRYAPGDAVEK 479 GRSLSLKL+QQLR+QS S + NK+ + + + S RTL E+SL E IRYA GD +E Sbjct: 418 GRSLSLKLLQQLREQSRI—YSGSGNNKSDSQSHI-SGRTLKEISLDEPIRYAMGDRIEL 474 Query: 480 WLNDLLCLDCLN-ITRIVS-GCPLPEACELYYVNRDTLFCYHKASEVFLQRLMALYVASH 537 WLN LLCLD + ++R+ + G P P C LY V+RDTLF YH SE FLQR+M+LYVASH Sbjct: 475 WLNKLLCLDAASYVSRMATQGFPHPSECSLYRVSRDTLFSYHPISEAFLQRMMSLYVASH 534 Query: 538 YKNSPNDLQMLSDAPAHHLFCLLPPVPPTQNALPEVLAVIQVCLEGEISRQSILNSLSRG 597 YKNSPNDLQ++SDAPAH LF LLPPV LP+ + VIQ+ LEG ISR+SI+NSLSRG Sbjct: 535 YKNSPNDLQLMSDAPAHQLFVLLPPVDLKNPKLPDPICVIQLALEGSISRESIMNSLSRG 594 Query: 598 KKASGDLIPWTVSEQFQDPDFGGLSGGRVVRIAVHPDYQGMGYGSRALQLLQMYYEGRFP 657 ++A GDLIPW +S+QFQD +F L G R+VRIAV P++ MGYG+RA+QLL Y+EG+F Sbjct: 595 QRAGGDLIPWLISQQFQDENFAALGGARIVRIAVSPEHVKMGYGTRAMQLLHEYFEGKFI 654 Query: 658 CLEEKVLETPQEIHTVSSEAV SLLEEVITPR—KDLPPLLLKLNERPAERLDYLGVS 712 E+ + + E + +L E I R K +PPLLLKL+E E L Y+GVS Sbjct: 655 SASEEFKAVKHSLKRIGDEEIENTALQTEKIHVRDAKTMPPLLLKLSELQPEPLHYVGVS 714 Query: 713 YGLTPRLLKFWKRAGFVPVYLRQTPNDLTGEHSCIMLKTLTDEDEADQGGWLAAFWKDFR 772 YGLTP L KFWKR G+ P+YLRQT NDLTGEH+C+ML+ L D WL AF ++F Sbjct: 715 YGLTPSLQKFWKREGYCPLYLRQTANDLTGEHTCVMLRVLEGRDSE WLGAFAQNFY 770 Query: 773 RRFLALLSYQFSTFSPSLALNIIQNRNMGKP AQPALSREELEALFLPYDLKRLEMY 828 RRFL+LL YQF F+ AL+++ N G + L+ EE+ +F YDLKRLE Y Sbjct: 771 RRFLSLLGYQFREFAAITALSVLDACNNGTKYVVNSTΞKLTNEEINNVFESYDLKRLESY 830 Query: 829 SRNMVDYHLIMDMIPAISRIYFLNQLGD-LALSAAQSALLLGIGLQHKSVDQLEKEIELP 887 S N++DYH+I+D++P ++ +YF + D + LS Q ++LL +GLQ+K++D LEKE LP Sbjct: 831 SNNLLDYHVIVDLLPKLAHLYFSGKFPDSVKLSPVQQSVLLALGLQYKTIDTLEKEFNLP 890 Query: 888 SGQLMGLFNRIIRKVVKLFNEVQEKAIEEQMVAAKDVVME PTMKTLSDDLDE 939 S QL+ + ++ +K++K +E++ K IEE++ + K P ++L ++L E Sbjct: 891 SNQLLAMLVKLSKKIMKCIDEIETKDIEEELGSNKKTESSNSKLPEFTPLQQSLEEELQE 950 Query: 940 AAKEFQ-EKHKKEVGKLKSMDLSEYIIRGDDEEWNEVLNKAGPNASIISLKSDKKRKLEA 998 A E +K+ + ++DL +Y IRG++E+W KA N I R + Sbjct: 951 GADEAMLALREKQRELINAIDLEKYAIRGNEEDW KAAEN-QIQKTNGKGARVVSI 1004 Query: 999 KQEPKQSKKL--KNRETKNKKDMKLKRKK 1025 K E +++ L +++TK K K K +K Sbjct: 1005 KGEKRKNNSLDASDKKTKEKPSSKKKFRK 1033 Pedant information for DKFZphtes3_6cll, frame 3 Report for DKFZphtes3_6cll .3 [LENGTH] 1025 [MW] 115704.57 [pi] 8.50 [HOMOL] PIR:S55151 probable membrane protein YNL132w - yeast (Saccharomyces cerevisiae) 0.0 [FUNCAT] 10.99 other signal-transduction activities [S. cerevisiae, YNL132w] 0.0 [FUNCAT] r general function prediction [H. influenzae, HI1254] 2e-05 [PROSITE] ATP_GTP_A 1 [PROSITE] RGD 1 [KW] Alpha_Beta [KW] LOW_COMPLEXITY 11.80 % SEQ MHRKKVDNRIRILIENGVAERQRSLFVVVGDRGKDQVVILHHMLSKATVKARPSVLWCYK SEG PRD cccccccchhhhhhcccccccceeeeeeeeccccceeeeehhhhhhhhhhccceeehhhh SEQ KELGFSSHRKKRMRQLQKKIKNGTLNIKQDDPFELFIAATNIRYCYYNETHKILGNTFGM SEG PRD hhhcccchhhhhhhhhhhhhhhhcccccccccceeeecccceeeeeccccceeeccccee SEQ CVLQDFEALTPNLLARTVETVEGGGLVVILLRTMNSLKQLYTVTMDVHSRYRTEAHQDVV SEG xxxxxxxxxxxxxxx PRD eehhhhhccccchhhhhhhhhcccceeeeeeccchhhhhhhhhhhhhhhhhhhhhhhhhh SEQ GRFNERFILSLASCKKCLVIDDQLNILPISSHVATMEALPPQTPDESLGPSDLELRELKE SEG PRD hhhhhhhhhhhcccceeeeeecceeeecccccccccccccccccccccccchhhhhhhhh SEQ SLQDTQPVGVLVDCCKTLDQAKAVLKFIEGISEKTLRSTVALTAARGRGKSAALGLAIAG SEG xxxxxxxxx PRD hhcccccceeeeehhhhhhhhhhhhhhhhhhhhhhhhhhheeeccccccchhhhhhhhhh SEQ AVAFGYSNIFVTSPSPDNLHTLFEFVFKGFDALQYQEHLDYEIIQSLNPEFNKAVIRVNV SEG xxx PRD hhhhcccceeecccccccchhhhhhhhhhhhhhhhhhhhhheeeeeccccccceeeeeeh SEQ FREHRQTIQYIHPADAVKLGQAELVVIDEAAAIPLPLVKSLLGPYLVFMASTINGYEGTG SEG PRD hhhhhhheeeeccccccccccceeeehhhhhccchhhhhhhccceeeeeeeccccccccc SEQ RSLSLKLIQQLRQQSAQSQVSTTAENKTTTTARLASARTLHEVSLQESIRYAPGDAVEKW SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD cchhhhhhhhhhhhhhhhhhhhccccccccccchhhhhhhhhhhhhhceeeccccchhhh SEQ LNDLLCLDCLNITRIVSGCPLPEACELYYVNRDTLFCYHKASEVFLQRLMALYVASHYKN SEG xxxxxxxxxxx PRD hhhhhhcccccceeeccccccccceeeeeeeccccccccchhhhhhhhhhhhhhhhhccc SEQ SPNDLQMLSDAPAHHLFCLLPPVPPTQNALPEVLAVIQVCLEGEISRQSILNSLSRGKKA SEG PRD cccccccccccccceeeeeeccccccccccchhhhhhhhhhccccchhhhhhhhcccccc SEQ SGDLIPWTVSEQFQDPDFGGLSGGRVVRIAVHPDYQGMGYGSRALQLLQMYYEGRFPCLE SEG PRD cccchhhhhhhhhhhccccccccceeeeeeccccccccccchhhhhhhhhhhhcccchhh SEQ EKVLETPQEIHTVSSEAVSLLEEVITPRKDLPPLLLKLNERPAERLDYLGVΞYGLTPRLL SEG xxxxxxxxxx PRD hhhhhccccccchhhhhhhhhhhhhhccccccccccccccccccceeeeccccccchhhh SEQ KFWKRAGFVPVYLRQTPNDLTGEHSCIMLKTLTDEDEADQGGWLAAFWKDFRRRFLALLS SEG PRD hhhhhcccceeeeeccccccccceeeeeeecccccccccchhhhhhhhhhhhhhhhhhhh SEQ YQFSTFSPSLALNIIQNRNMGKPAQPALSREELEALFLPYDLKRLEMYSRNMVDYHLIMD SEG PRD hhhhcchhhhhhhhhhhcccccccchhhhhhhhhhhhccchhhhhhhhhccchhhhhhhh SEQ MIPAISRIYFLNQLGDLALSAAQSALLLGIGLQHKSVDQLEKEIELPSGQLMGLFNRIIR SEG xxxxxxxxxxxxxxxxxxxxx PRD hhhhhhhhhhhhcccchhhhhhhhhhhhhhcchhhhhhhhhhhhhccccchhhhhhhhhh SEQ KVVKLFNEVQEKAIEEQMVAAKDVVMEPTMKTLSDDLDEAAKEFQEKHKKEVGKLKSMDL SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhcccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhcc SEQ SEYIIRGDDEEWNEVLNKAGPNASIISLKSDKKRKLEAKQEPKQSKKLKNRETKNKKDMK SEG xxxxxxxxxxxxxxx PRD cceeecccchhhhhhhhhccccceeeeeeccchhhhhhhhcccccccccccccccchhhh SEQ LKRKK SEG xxxxx PRD hhccc Prosite for DKFZphtes3_6cll .3 PS00016 966->969 RGD PDOC00016 PS00017 284->292 ATP GTP A PDOC00017 (No Pfam data available for DKFZphtes3_6cll .3) DKFZphtes3_6dl6 group: testes derived DKFZphtes3_6dl6 encodes a novel 695 ammo acid protein nearly identical to a sequence from human PAC clone WUGSC:H_DJ1185I07.2. The cDNA is different to the proposed gene model: it contains additional exons. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . WUGSC:H_DJ1185I07.2, differences to genmodel differences to genmodel of WUGSC:H_DJ1185I07.2 two exons skippt, Sequenced by BMFZ Locus: /map="7qll .23-q21" Insert length: 4572 bp Poly A stretch at pos. 4540, polyadenylation signal at pos. 4520 1 GGCGGCGCTA GCTTCGGAGT CTCCCGCGCG CACCTCAGCC GCCTCCTAGC 51 GGCGCGGCGC TCGCTCCTAC GCCTAAAATG ACCAATGTGT GATTTCAGTG 101 GAATAAATGG CGTCCAAAGT CACAGATGCT ATAGTCTGGT ATCAAAAGAA 151 GATTGGAGCA TATGATCAAC AAATATGGGA AAAATCTGTT GAACAGAGAG 201 AAATCAAGGG GCTAAGGAAT AAACCAAAGA AAACAGCACA TGTGAAACCA 251 GACCTCATAG ATGTTGATCT TGTAAGAGGG TCTGCATTTG CAAAGGCAAA 301 GCCTGAAAGT CCTTGGACTT CTCTGACCAG AAAGGGAATT GTTCGAGTTG 351 TATTTTTCCC CTTTTTCTTC CGGTGGTGGT TACAAGTAAC ATCAAAGGTC 401 ATCTTTTTCT GGCTTCTTGT CCTTTATCTT CTTCAAGTTG CTGCAATAGT 451 ATTATTCTGC TCCACTTCTA GCCCACACAG CATACCTCTG ACAGAGGTGA 501 TTGGGCCGAT ATGGCTGATG CTGCTCCTGG GAACTGTGCA TTGCCAGATT 551 GTTTCCACAA GAACACCCAA ACCTCCTCTA AGTACAGGGG GTAAAAGAAG 601 AAGGAAATTA AGAAAAGCAG CCCATTTGGA AGTACATAGG GAAGGAGATG 651 GTTCTAGTAC CACAGATAAC ACACAAGAGG GAGCAGTTCA GAACCACGGT 701 ACAAGCACCT CTCACAGCGT TGGCACTGTC TTCAGAGATC TCTGGCATGC 751 TGCTTTCTTT TTATCAGGAT CAAAGAAAGC AAAGAATTCA ATTGATAAAT 801 CAACTGAAAC TGACAATGGC TATGTATCCC TTGATGGGAA GAAGACTGTT 851 AAAAGCGGTG AAGATGGAAT ACAAAACCAT GAACCTCAGT GTGAAACTAT 901 TCGACCAGAA GAGACAGCCT GGAACACAGG AACACTGAGG AATGGTCCTA 951 GCAAAGATAC CCAAAGGACA ATAACAAATG TCTCTGATGA AGTCTCCAGT 1001 GAGGAAGGTC CTGAAACAGG ATACTCATTA CGTCGTCATG TGGACAGGAC 1051 TTCTGAAGGT GTTCTTCGGA ATAGAAAGTC ACACCATTAT AAGAAACATT 1101 ACCCTAATGA GGACGCCCCT AAATCGGGTA CTAGTTGCAG CTCTCGCTGT 1151 TCAAGTTCCA GACAGGATTC TGAGAGTGCA AGGCCAGAAT CTGAAACAGA 1201 AGATGTGTTA TGGGAAGACT TGTTACATTG TGCAGAATGC CATTCATCTT 1251 GTACCAGTGA GACAGATGTG GAAAATCATC AGATTAATCC ATGTGTGAAA 1301 AAAGAATATA GAGATGACCC TTTTCATCAG AGTCATTTGC CCTGGCTCCA 1351 TAGTTCCCAC CCAGGATTAG AAAAAATAAG TGCTATAGTA TGGGAAGGTA 1401 ATGATTGTAA GAAAGCAGAC ATGTCTGTAC TTGAAATCAG TGGAATGATA 1451 ATGAACAGAG TGAACAGCCA TATACCAGGA ATAGGATACC AGATTTTTGG 1501 AAATGCAGTC TCTCTCATAC TGGGTTTAAC TCCATTTGTT TTCCGACTTT 1551 CTCAAGCTAC AGACTTGGAA CAACTCACAG CACATTCTGC TTCAGAACTT 1601 TATGTGATTG CATTTGGTTC TAATGAAGAT GTCATAGTTC TTTCTATGGT 1651 TATAATAAGT TTTGTGGTTC GCGTGTCTCT TGTGTGGATT TTCTTTTTTT 1701 TGCTCTGTGT AGCAGAAAGA ACTTATAAAC AGCGATTACT TTTTGCAAAA 1751 CTCTTTGGAC ATTTAACATC TGCAAGGAGG GCTCGAAAAT CTGAGGTTCC 1801 TCATTTCCGG TTGAAGAAAG TACAGAATAT AAAAATGTGG CTATCTCTCC 1851 GTTCCTATCT TAAGCGTCGA GGTCCTCAGC GATCAGTTGA TGTAATAGTT 1901 TCATCTGCTT TCTTATTGAC TATCTCAGTT GTATTTATCT GTTGTGCCCA 1951 GATAAACCTC TACTTGAAAA TGGAGAAAAA ACCTAACAAA AAGGAGGAAC 2001 TGACACTAGT GAATAATGTT TTAAAACTGG CTACTAAACT GCTAAAGGAG 2051 TTGGACAGTC CTTTTAGATT ATATGGGCTT ACAATGAATC CGCTGCTTTA 2101 TAACATCACC CAGGTTGTTA TCCTGTCAGC TGTTTCTGGT GTTATCAGTG 2151 ACTTGCTTGG ATTTAATTTA AAGCTATGGA AGATTAAGTC ATGACAATTC 2201 AAAGAAAAGA AGATGTAGCC TCTTTTCCAG AATAAGAGTA CTGACTAAGC 2251 TGCCTGAAAG CTTGTCACTG ATTCTTTGCT TCAGGAGTCT CAGCTAGGGA 2301 GTTGAAGTGT TTACATCAGA CTGTCTTGTG CAATTCTTAT ATTTATTTTA 2351 CTGGTTCACT TTTTTTTACA TTTATTTTAG TCTTTATATT TTTATTTTTA 2401 AGCATTGATG TACTTAGTTG TTGAAAGGGT GATGAAACTG ATATCCAGAT 2451 ACTTGAGATC CTGGTAATTG GTCATAAATA ATTGGCAAAA TAACAAATTG 2501 TGAAAATAGA AGCCATTGCT CAGCACCGTT TCTCCATCAA TGCCGTGAAC 2551 TTGCCTTACT TGAGGAAAAA TTCTTTAACT TTGGAATATT GCATTGAACT 2601 CAGCTATACA CATAAAACAT TTTCTTTGGT AAATCAAGAT CCAGTCAGGG 2651 TTTCTCTTGA ATTATTTTGG AACAATGCCA GGATCCAAAC TGATTAAGTT 2701 ACAGTTTAAG CACCCTTCAG TATTAATATA TACGGTATTA TATAACAGGT 2751 CAACAAGTGC TCTTTGATGA TAAAACTTGT AATAGAGCAA TAATTGTAAA 2801 TGGTTACCAT ACTGTAAGAT ATTTTGATAA AAATTAACTA GTAATACTTG 2851 TATTTATTTG AAACACTGGG CTGTTTGCAC AGCTCCAACT GTGCATGCTC 2901 AAAATGTGCA CTTTTTAAAA TTGTTACTTT TAATGCGTAT CTTTATATGG 2951 GATCTGTTAT AGTATACTAG GGCATGATAT GGTATCCTTT TGAGTGAGGT 3001 ATATACTCAT CTCACAAGTG AAGTGCCTAC TGATATTACT AAAGTACATT 3051 ATGTTTACTC AAGTAAATAA TTTTCTCCCC ATGGTACACT CTAGTGTAGG 3101 CTATTCATAC CACACTGAAA TGAACAACTG AAGAATAAGG CTAAGAACCA 3151 ATAAAATATT TCTCTAATTG CTAGTTGTAA AACTGTATCC AAATTTTCAG 3201 AAAAGACAGC TTCAGCTTGC AAATTCTATC CTCTAAACTT ATCTGGTGCA 3251 TTCTCCCCAC CCCACCCCCA TTATATAAGG GCTATTTTAG ATGCTTTTAA 3301 CCTCCCCAAC AAATAATTTG CCAAGTGTCC AATGAGAACT TATCATGTTG 3351 GTGTGTTAGG TAAATCGGGC AAATATGATA GTGTCTTACA TTGGGCCTTG 3401 ATTTTAAGTT GTTATATTTG TACAATCGAG TATTTTAGAA ATTACATGAA 3451 ACATGAAACA GTTTTTGCAA TTTTTTTTAA ACTGGGCATC TGGTTTCTAA 3501 AAATTTATTT GAAACAATCT AGAATTTTCT TGGTGCAAAG TGTATCATGT 3551 GGAATATCCT CATATTTTTA CCATATTTTA AGAACTTTAA GACGATTAAT 3601 TGTAAATAAT TTATTTGATT GGTGCAGTTC TAATCCCTAA ATCATAATCT 3651 TAAAATCAGG AATGTGTGGA GAACAGAGCC ATGTCATATC ACTTTGCTCT 3701 TACCATTCCT TTTGATCAGC CTCAATTCAG CCTCATTGTG TAGTATGTTT 3751 TTTCTTTCTA TGAAAAACAA CAGAAAGCAT TTCATTTTAT TTGCCTATGT 3801 TCAAATATGT TTAATAATGA CCAAAGTGCA TTCTGAGTTT TTTCAAGGAA 3851 TGTAATACTG GAGCTTTAAG AACATACTTA GTTTCTCATG TGAAAACTTA 3901 GGCTTTGTCT GATGTTTTTC CTTCCTCTAT TGTCTAATGT TGAGGTTGTT 3951 TTTAGGAATT ATGTTTTATA AACTTTTTCA ATATAAGGTA CATGCCTATA 4001 CAGAACTTAA CATTTTGCAC AGAATATATC AAATATATTT TGAGAAAAAA 4051 AGTACGGCAT GAGTTCTGTT AGGAATAAAA GATGAAACTA TTGTATCTCA 4101 CAAAAAATCT TATTTCAGAA TGGAAATATT TTTGAGAAAA GTAGCTGAGT 4151 ATACTGGTTT AAGAAAATGC TTGTTTTAGA TTGAGGTTAA CTTAGAGTTG 4201 GGAGTTGATT TATTAAGTAC AGTATACCTC TCAACAGTTT ATAAATAATA 4251 TGTTGAATTA TGTCAGTGTG GGCAGCAGTA GAATACTAAA AGGAAAATGT 4301 CATGTTAAGC AATTTCAGAA CATTAACTGA ACTATTTTCA AAGCAGAAAA 4351 ATTGACATTG CTGCCTTTAA GAATACCATG AATGTAAGAA ATTGAAAGAA 4401 ATTGTAAAAT ATCACATAAT ATAGAAATGG CAGTTCAAAG AGAATTGTGG 4451 CAGATGTTGT GTGTGAACTG TTGTTTCTTT GCCACATGTG TTGTATTTGA 4501 AAGTTTTACA GTAAGTTTAA AATAAAACAT TCTGTGACTG AAAAAAAAAA 4551 AAAAAAAAAA AAAAAAAAAA AA BLAST Results No BLAST result Medline entries No Medlme entry Peptide information for frame 2 ORF from 107 bp to 2191 bp; peptide length: 695 Category: known protein Classification: unclassified Prosite motifs: CYTOCHROME C (375-381) 1 MASKVTDAIV WYQKKIGAYD QQIWEKSVEQ REIKGLRNKP KKTAHVKPDL 51 IDVDLVRGSA FAKAKPESPW TSLTRKGIVR VVFFPFFFRW WLQVTSKVIF 101 FWLLVLYLLQ VAAIVLFCST SSPHSIPLTE VIGPIWLMLL LGTVHCQIVS 151 TRTPKPPLST GGKRRRKLRK AAHLEVHREG DGSSTTDNTQ EGAVQNHGTS 201 TSHSVGTVFR DLWHAAFFLS GSKKAKNSID KSTETDNGYV SLDGKKTVKS 251 GEDGIQNHEP QCETIRPEET AWNTGTLRNG PSKDTQRTIT NVSDEVSSEE 301 GPETGYSLRR HVDRTSEGVL RNRKSHHYKK HYPNEDAPKS GTSCSSRCSS 351 SRQDSESARP ESETEDVLWE DLLHCAECHS SCTSETDVEN HQINPCVKKE 401 YRDDPFHQSH LPWLHSSHPG LEKISAIVWE GNDCKKADMS VLEISGMIMN 451 RVNSHIPGIG YQIFGNAVSL ILGLTPFVFR LSQATDLEQL TAHSASELYV 501 IAFGSNEDVI VLSMVIISFV VRVSLVWIFF FLLCVAERTY KQRLLFAKLF 551 GHLTSARRAR KSEVPHFRLK KVQNIKMWLS LRΞYLKRRGP QRSVDVIVSS 601 AFLLTISVVF ICCAQINLYL KMEKKPNKKE ELTLVNNVLK LATKLLKELD 651 SPFRLYGLTM NPLLYNITQV VILSAVSGVI SDLLGFNLKL WKIKS BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_6dl6, frame 2 PIR:S38170 SRP40 protein - yeast (Saccharomyces cerevisiae), N = 1, Score = 100, P = 0.08 TREMBL :AC004990_1 gene: "WUGSC:H_DJ1185I07.2"; Homo sapiens PAC clone DJ1185I07 from 7qll.23-q21, complete sequence., N = 2, Score = 2693, P = 0 >TREMBL:AC004990_1 gene: "WUGSC:H_DJ1185I07.2"; Homo sapiens PAC clone DJ1185I07 from 7qll.23-q21, complete sequence. Length = 588 HSPs: Score = 2693 (404.1 bits), Expect = O.Oe+00, Sum P(2) = O.Oe+00 Identities = 510/515 (99%), Positives = 512/515 (99%) Query: 35 GLRNKPKKTAHVKPDLIDVDLVRGSAFAKAKPESPWTSLTRKGIVRVVFFPFFFRWWLQV 94 GLRNKPKKTAHVKPDLIDVDLVRGSAFAKAKPESPWTSLTRKGIVRVVFFPFFFRWWLQV Sbjct: 1 GLRNKPKKTAHVKPDLIDVDLVRGSAFAKAKPESPWTSLTRKGIVRVVFFPFFFRWWLQV 60 Query: 95 TSKVIFFWLLVLYLLQVAAIVLFCSTSSPHSIPLTEVIGPIWLMLLLGTVHCQIVSTRTP 154 TSKVIFFWLLVLYLLQVAAIVLFCSTSSPHSIPLTEVIGPIWLMLLLGTVHCQIVΞTRTP Sbjct: 61 TSKVIFFWLLVLYLLQVAAIVLFCSTSSPHSIPLTEVIGPIWLMLLLGTVHCQIVSTRTP 120 Query: 155 KPPLSTGGKRRRKLRKAAHLEVHREGDGSSTTDNTQEGAVQNHGTSTSHSVGTVFRDLWH 214 KPPLSTGGKRRRKLRKAAHLEVHREGDGSSTTDNTQEGAVQNHGTSTSHSVGTVFRDLWH Sbjct: 121 KPPLSTGGKRRRKLRKAAHLEVHREGDGSΞTTDNTQEGAVQNHGTSTSHSVGTVFRDLWH 180 Query: 215 AAFFLSGSKKAKNSIDKSTETDNGYVSLDGKKTVKSGEDGIQNHEPQCETIRPEETAWNT 274 AAFFLSGSKKAKNSIDKSTETDNGYVSLDGKKTVKSGEDGIQNHEPQCETIRPEETAWNT Sbjct: 181 AAFFLSGSKKAKNSIDKSTETDNGYVSLDGKKTVKSGEDGIQNHEPQCETIRPEETAWNT 240 Query: 275 GTLRNGPSKDTQRTITNVSDEVSSEEGPETGYSLRRHVDRTSEGVLRNRKSHHYKKHYPN 334 GTLRNGPSKDTQRTITNVSDEVSSEEGPETGYSLRRHVDRTSEGVLRNRKSHHYKKHYPN Sbjct: 241 GTLRNGPSKDTQRTITNVSDEVSSEEGPETGYSLRRHVDRTSEGVLRNRKSHHYKKHYPN 300 Query: 335 EDAPKSGTSCSSRCSSSRQDSESARPESETEDVLWEDLLHCAECHSSCTSETDVENHQIN 394 EDAPKSGTSCSSRCSSSRQDSESARPESETEDVLWEDLLHCAECHSSCTSETDVENHQIN Sbjct: 301 EDAPKSGTΞCSSRCSSSRQDSESARPESETEDVLWEDLLHCAECHSSCTΞETDVENHQIN 360 Query: 395 PCVKKEYRDDPFHQSHLPWLHSSHPGLEKISAIVWEGNDCKKADMSVLEISGMIMNRVNS 454 PCVKKEYRDDPFHQSHLPWLHSSHPGLEKISAIVWEGNDCKKADMSVLEISGMIMNRVNS Sbjct: 361 PCVKKEYRDDPFHQSHLPWLHSSHPGLEKISAIVWEGNDCKKADMSVLEISGMIMNRVNS 420 Query: 455 HIPGIGYQIFGNAVSLILGLTPFVFRLSQATDLEQLTAHSASELYVIAFGSNEDVIVLSM 514 HIPGIGYQIFGNAVSLILGLTPFVFRLSQATDLEQLTAHSASELYVIAFGSNEDVIVLSM Sbjct: 421 HIPGIGYQIFGNAVSLILGLTPFVFRLSQATDLEQLTAHSASELYVIAFGSNEDVIVLSM 480 Query: 515 VIISFVVRVSLVWIFFFLLCVAERTYKQRLLFAKL 549 VIISFVVRVSLVWIFFFLLCVAERTYKQ L+ K+ Sbjct: 481 VIISFVVRVΞLVWIFFFLLCVAERTYKQINLYLKM 515 Score = 409 (61.4 bits), Expect = O.Oe+00, Sum P(2) = O.Oe+00 Identities = 92/115 (80%), Positives = 98/115 (85%) Query: 595 DVIVSS AFLLTISVVFI CCA QINLYLKMEKKPNKKEELTLVNNVLK 640 DVIV S +F++ +S+V+I C A QINLYLKMEKKPNKKEELTLVNNVLK Sbjct: 474 DVIVLSMVIISFVVRVSLVWIFFFLLCVAERTYKQINLYLKMEKKPNKKEELTLVNNVLK 533 Query: 641 LATKLLKELDSPFRLYGLTMNPLLYNITQVVILSAVSGVISDLLGFNLKLWKIKS 695 LATKLLKELDSPFRLYGLTMNPLLYNITQVVILSAVΞGVISDLLGFNLKLWKIKS Sbjct: 534 LATKLLKELDSPFRLYGLTMNPLLYNITQVVILSAVSGVISDLLGFNLKLWKIKS 588 Pedant information for DKFZphtes3_6dl6, frame 2 Report for DKFZphtes3_6dl6.2 [LENGTH] 695 [MW] 78466.68 [pi] 9.30 [HOMOL] TREMBL :AC004990_1 gene: "WUGSC :H_DJ1185I07.2"; Homo sapiens PAC clone DJ1185I07 from 7qll.23-q21, complete sequence. 0.0 [PROSITE] CYTOCHROME_C 1 [KW] TRANSMEMBRANE 6 [KW] LOW COMPLEXITY 5.32 SEQ MASKVTDAIVWYQKKIGAYDQQIWEKSVEQREIKGLRNKPKKTAHVKPDLIDVDLVRGSA SEG PRD ccceeeeehhhhhhhcccchhhhhhhhhhhhhhhcccccccccccccccceeeeeeccch MEM SEQ FAKAKPEΞPWTSLTRKGIVRVVFFPFFFRWWLQVTSKVIFFWLLVLYLLQVAAIVLFCST SEG xxxxxxxxxxx PRD hhhhcccccccccccccceeeeecchhhhhhhhhhhhhhhhhhhhhhhhhhhhhheeecc MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM SEQ SSPHSIPLTEVIGPIWLMLLLGTVHCQIVSTRTPKPPLSTGGKRRRKLRKAAHLEVHREG SEG xxxxxxxx PRD ccccccceeeeehhhhhhhhhhhhheeeeeeccccccccccchhhhhhhhhhhhheeecc MEM MMMMMMMMMMMMMMMMMMMMMMM SEQ DGSSTTDNTQEGAVQNHGTSTSHSVGTVFRDLWHAAFFLSGSKKAKNSIDKSTETDNGYV SEG PRD cccccccccceeeeeeccccccccchhhhhhhhhhhhhhcccchhhhhcccccccccccc MEM SEQ SLDGKKTVKSGEDGIQNHEPQCETIRPEETAWNTGTLRNGPSKDTQRTITNVSDEVΞSEE SEG PRD cccccceeecccccccccccccccccccceeeeccccccccccccceeeecccccccccc MEM SEQ GPETGYΞLRRHVDRTSEGVLRNRKSHHYKKHYPNEDAPKSGTSCSSRCSSSRQDSESARP SEG xxxxxxxxxxxxxxxxxx ... PRD ccccceeeeeeccccccchhhhhhcccccccccccccccccccccccccccccccccccc MEM SEQ ESETEDVLWEDLLHCAECHSSCTSETDVENHQINPCVKKEYRDDPFHQSHLPWLHSSHPG SEG PRD cccchhhhhhhhhhhhcccccccccccccccccccceeeeeccccccccccccccccccc MEM SEQ LEKISAIVWEGNDCKKADMSVLEISGMIMNRVNSHIPGIGYQIFGNAVSLILGLTPFVFR SEG PRD cccceeeeeecccccccceeeeehhhhhhhhhccccccccccccccccceeecccccchh MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM SEQ LSQATDLEQLTAHSASELYVIAFGSNEDVIVLSMVIISFVVRVSLVWIFFFLLCVAERTY SEG PRD hhhhhhhhhhhhcccceeeeeeeccccceeeehhhhhhhhcchhhhhhhhhhhhhhhhhh MEM MMMMMMMMMMMMMMMMMMMMMMMMMMMMM SEQ KQRLLFAKLFGHLTSARRARKSEVPHFRLKKVQNIKMWLSLRSYLKRRGPQRSVDVIVSS SEG PRD hhhhhhhhhhhhhhhhhhhhhhccccccceeeeeehhhhhhhhhhhhccccceeeeeeee MEM MMMMMMM SEQ AFLLTISVVFICCAQINLYLKMEKKPNKKEELTLVNNVLKLATKLLKELDSPFRLYGLTM SEG PRD eeeeeeeeeeeeeehhhhhhhhhhcccchhhhhhhhhhhhhhhhhhhhccccceeeeccc MEM MMMMMMMMMMMMMMMMMMM SEQ NPLLYNITQVVILSAVSGVISDLLGFNLKLWKIKS SEG PRD cchhhhheeeeeeeeecchhhhhccceeeeeeccc MEM MMMMMMMMMMMMMMMMMMMMMMMMM Prosite for DKFZphtes3_6dl6.2 PS00190 375->381 CYTOCHROME C PDOC00169 (No Pfam data available for DKFZphtes3_6dl6.2) DKFZphtes3_72kll group: testes derived DKFZphtes3_72kll encodes a novel 233 ammo acid protein with similarity to S. pombe hypothetical repeat-containing protein. The novel protein contains 5 leucine zippers and a microbodies C-terrainal targeting signal (S- K-L) signature. This sequence is responsible for transport of proteins from free polysomes into the microbodies. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . similarity to S. pombe hypothetical repeat-containing protein complete cDNA, complete eds, 6 EST hits (3 from testis derived librarys) Sequenced by DKFZ Locus : unknown Insert length: 1134 bp Poly A stretch at pos. 1124, polyadenylation signal at pos. 1088 1 AACCTTTCAA GTGCCCCCTC CTTTCCTTAA AGTCTTTTAT AGGGGTCCCC 51 TTCTTGGCCA TCTCCATCCT GTGAGTCAGG ACTGAAAGGG CACAGACAGG 101 TCACTGCCAG CATTGTTGGG GCAAGCCTGC AAGCACGCAT CACTGGGGAT 151 CTGACATGAC AATGGCCGCC TGCCCCCTCT GAGGGCTACA GGACTTACCC 201 CAGTGGGAAG CAGCTAAGCA GGTCTGACCA GCCGACCTGG ACCTGGCCAA 251 GGGTCCTGTC ATCCCTCATG GCCACCCCGC CATTCCGGCT GATAAGGAAG 301 ATGTTTTCCT TCAAGGTGAG CAGATGGATG GGGCTTGCCT GCTTCCGGTC 351 CCTGGCGGCA TCCTCTCCCA GTATTCGCCA GAAGAAACTA ATGCACAAGC 401 TGCAGGAGGA AAAGGCTTTT CGCGAAGAGA TGAAAATTTT TCGTGAAAAA 451 ATAGAGGACT TCAGGGAAGA GATGTGGACT TTCCGAGGCA AGATCCATGC 501 TTTCCGGGGC CAGATCCTGG GTTTTTGGGA AGAGGAGAGA CCTTTCTGGG 551 AAGAGGAGAA AACCTTCTGG AAAGAGGAAA AATCCTTCTG GGAAATGGAA 601 AAGTCTTTCA GGGAGGAAGA GAAAACTTTC TGGAAAAAGT ACCGCACTTT 651 CTGGAAGGAG GATAAGGCCT TCTGGAAAGA GGACAATGCC TTATGGGAAA 701 GAGACCGGAA CCTTCTTCAG GAGGACAAGG CCCTGTGGGA GGAAGAAAAG 751 GCCCTGTGGG TAGAGGAAAG AGCCCTCCTT GAGGGGGAGA AAGCCCTGTG 801 GGAAGATAAA ACGTCCCTCT GGGAGGAAGA GAATGCCCTC TGGGAGGAAG 851 AGAGGGCCTT CTGGATGGAG AACAATGGCC ACGTTGCCGG AGAGCAGATG 901 CTCGAAGATG GGCCCCACAA CGCCAACAGA GGGCAGCGCT TGCTGGCCTT 951 CTCCCGAGGC AGGGCGTAGC CAGCATGCAG GTGCAGGGCC CTGTGGTCCA 1001 GACTCCCCTG GGTTGGGATT CAAGTCCAGG GTGAGCCCAT GTGCTGGAGA 1051 AAATACACAC TCATTGGTCT CCTTGCTTTG AAAGATCCAA TAAAGTCCTG 1101 AGGCAAGGTT TGGAAAACCA ACTTAAAAAA AAAA BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 1 ORF from 268 bp to 966 bp; peptide length: 233 Category: similarity to known protein Prosite motifs: MICROBODIES_CTER (231-234) LEUCINE_ZIPPER (142-164) LEUCINE_ZIPPER (149-171) LEUCINE_ZIPPER (156-178) LEUCINE_ZIPPER (163-185) LEUCINE_ZIPPER (170-192) LEUCINE ZIPPER (170-192) 1 MATPPFRLIR KMFSFKVSRW MGLACFRSLA ASSPSIRQKK LMHKLQEEKA 51 FREEMKIFRE KIEDFREEMW TFRGKIHAFR GQILGFWEEE RPFWEEEKTF 101 WKEEKSFWEM EKSFREEEKT FWKKYRTFWK EDKAFWKEDN ALWERDRNLL 151 QEDKALWEEE KALWVEERAL LEGEKALWED KTSLWEEENA LWEEERAFWM 201 ENNGHVAGEQ MLEDGPHNAN RGQRLLAFSR GRA BLASTP hits Entry SPCC330_4 from database TREMBLNEW: gene: "SPCC330.04c"; product: "hypothetical repeat-containing protein" S. pombe chromosome III cosmid c330. Score = 149, P = 1.6e-08, identities = 55/187, positives = 88/187 Entry A45973 from database PIR: trichohyalm - human Score = 147, P = 3.0e-07, identities = 57/194, positives = 94/194 Alert BLASTP hits for DKFZphtes3_72kll, frame 1 No Alert BLASTP hits found Pedant information for DKFZphtes3_72kll, frame 1 Report for DKFZphtes3_72kll .1 [LENGTH] 233 [MW] 28752.65 [pi] 5.70 [PROSITE] LEUCINE_ZIPPER 5 [PROSITE] MICROBODIES_CTER [PROSITE] MYRISTYL 1 [PROSITE] CK2_PHOSPHO_SITE [PROSITE] PKC_PHOSPHO_SITE [KW] All_Alpha [KW] LOW COMPLEXITY 15.45 SEQ MATPPFRLIRKMFSFKVSRWMGLACFRSLAASSPSIRQKKLMHKLQEEKAFREEMKIFRE SEG PRD cccccchhhhhhhhhhhhhhhhhhhhhhhhhcccccchhhhhhhhhhhhhhhhhhhhhhh SEQ KIEDFREEMWTFRGKIHAFRGQILGFWEEERPFWEEEKTFWKEEKSFWEMEKSFREEEKT SEG xxxxxxxxxxxxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhcccccccccccccccccccchhhhhhhhhhhhhhhhhhhhhhhh SEQ FWKKYRTFWKEDKAFWKEDNALWERDRNLLQEDKALWEEEKALWVEERALLEGEKALWED SEG PRD hhhhcccccccccchhhhhhhhhhhhchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh SEQ KTSLWEEENALWEEERAFWMENNGHVAGEQMLEDGPHNANRGQRLLAFSRGRA SEG ... xxxxxxxxxxxx PRD ccchhhhhhhhhhhhhhhhhhccccchhhhhhcccccccccchhhhhhhhccc Prosite for DKFZphtes3_72kll .1 PS00005 14->17 PKC_PHOSPHO_SITE PDOC00005 PS00005 35->38 PKC_PHOS PHO_S ITE PDOC00005 PS00005 71->74 PKC_PHOS PHO_S ITE PDOC00005 PS00005 113-M16 PKC_PHOSPHO_SITE PDOC00005 PS00006 106->110 CK2_PHOSPHO_SITE PDOC00006 PS00006 113->117 CK2_PHOSPHO_SITE PDOC00006 PS00006 183->187 CK2_PHOSPHO_SITE PDOC00006 PS00008 81->87 MYRISTYL PDOC00008 PS00342 231->234 MICROBODIES_CTER PDOC00299 PS00029 142->164 LEUCINE_ZIPPER PDOC00029 PS00029 149-M71 LEUCINE_ZIPPER PDOC00029 PS00029 156->178 LEUCINE_ZIPPER PDOC00029 PS00029 163->185 LEUCINE_ZIPPER PDOC00029 PS00029 170->192 LEUCINE ZIPPER PDOC00029 (No Pfam data available for DKFZphtes3 72kll.l) DKFZphtes3_72kl5 group: cell structure and motility DKFZphtes3_72kl5 encodes a novel 188 amino acid protein with strong similarity to Rattus norvegicus actin-fllament binding protein Frabin. FGDl-related F-actin-binding protein (Farbm/FGDl) is a novel F-actm-bmding protein. The gene locus fgdl seems to be responsible for faciogemtal dysplasia or Aarskog-Scott syndrome. Frabin binds F-actin and shows F-actm-cross-linking activity. Overexpression of frabin in Swiss 3T3 cells and COS7 cells induces cell shape change and c-Jun N-terminal kinase activation, as described for FGDl. Because FGDl has been shown to serve as a GDP/GTP exchange protein for Cdc42 small G protein, it is likely that frabin is a direct linker between Cdc42 and the actin cytoskeleton. Cdc42p is an esin yeast, Cdc42p transduces signals to the actin cytoskeleton to initiate and maintain polarized growth and to mitogen-activated protein morphogenesis. In mammalian cells, Cdc42p regulates a variety of actm-dependent events and induces the JNK/SAPK protein kinase cascade, which leads to the activation of transcription factors within the nucleus. The novel protein seems to be the human orthologue of rat frabin. The new protein can find application in modulating of cell structure and motility as well as modulation of the JNK/SAPK pathway. strong similarity to act -filament binding protein Frabin Sequenced by DKFZ Locus : unknown Insert length: 1845 bp Poly A stretch at pos. 1835, polyadenylation signal at pos. 1816 1 GTGATGGAGA GTGCTGTTAT GATAGATGAA TCTAGGAAAG CCTCTTTGGA 51 GATGTGATAC CTGAACAGAA CCCCGAATGA TAAGAAGAAA TACCAGTGTT 101 TTAGGAGAGA TTGTCCTAAG CAGAGAACAG CAGCTGCAAA GACCCCAAGA 151 CACATACACT TGGTTATTAA GAATGGGAGC AGCAAGGAGT ATGGCAAGAA 201 CACAGTGAGT TTTCCCTTGA GTGTGTGAGG AAGCCCTCAG AGTTTGTGAC 251 TGACTTGTAG AGGTTCTAGT GGAGGGGATC AGAGTGGAAA CAAAGAGACC 301 AGTTAAAAAG GTATGGCAGC ATGAATAAAA AAGTTTTGAG AGTATTCATT 351 ATGCCTTCCA AATAAAAAAC TCTTTGGTTC ATAATTTGTT CATAAATTAA 401 GGACTGGCTA CACTGTACTA TTTAAAAATG TTAAGAAACA TCAATAAGTA 451 AAAATGTTAG GAAGAGATGA TAAATACGTA AGTATTATAT CTAACTAAGT 501 CTTTACTAAC TAGTCACATT ATTAAACAGT GCAAGGATCA AGAAAAGTTA 551 AGCGTTGAAA AATAAATAAA TAAGTTATAA ATAAAATAAA CAGCCCAAGG 601 AAATGTTCCA GTCCCCATAG GTAGACTCGG GGTCATCTTC TTTATTTAAA 651 TCTTTATTTA AATGTGGATA GCATCCCAAG AGACTTGGGT CTACACTAAG 701 AATATTCAAA TCCATGTTTC TGAAACCATC AGAGATAGAA AAAAAAAGTA 751 GCGAATATCC CTTTTCAACT GGAATAAACT TGTCTTAATT CTAGAACTTT 801 TCCATACCAA TGTTTTCATG CTTCCTTTGT ATTTTATCTT TTAGCTCATT 851 ATCAAATTAT AGTGATTTGA AGAAAGAGTC TGCTGTGAAC CTAAATGCTC 901 CTAGAACCCC AGGAAGGCAT GGATTGACAA CCACACCTCA ACAAAAACTC 951 CTCTCCCAGC ACTTGCCACA GAGGCAGGGA AATGATACAG ATAAGACTCA 1001 GGGTGCACAG ACTTGTGTGG CCAACGGTGT AATGGCAGCA CAAAACCAGA 1051 TGGAATGTGA GGAGGAGAAA GCTGCCACTC TTAGCTCAGA TACTTCTATT 1101 CAAGCTTCTG AACCCTTGCT TGATACGCAC ATAGTGAATG GAGAAAGAGA 1151 TGAAACTGCC ACAGCTCCTG CATCACCCAC AACAGATAGC TGTGATGGAA 1201 ATGCTTCTGA CAGTAGCTAC AGGACTCCAG GCATAGGCCC AGTGCTCCCC 1251 CTAGAAGAAA GAGGGGCAGA AACAGAAACC AAGGTACAAG AGAGGGAAAA 1301 TGGGGAAAGC CCTCTGGAAC TGGAGCAGCT GGACCAGCAC CATGAGATGA 1351 AGGTAGAGCA TGAGACTAGC TCATGAGCAG GGAAAACCCT GCCTATTCGA 1401 TTGTTGTCTT AAAACTCTTT ATTTATTGCA CCCCTGAAAT GTATGAATCA 1451 GATCACCCAC ACTGGCAGTT AAACGATTTT CAAGCTCTGG CTGCTGATTA 1501 GCATTTCCCC TATGCTCTAA GCAGATATTT CACTTTTTCT TTTCATGTAG 1551 TTTCTGTTAA TATCTCTGTT GTAATTTCAG GAGTCAGAAC AGTGTGGAAA 1601 CTTTAATATA GGAAATCCAC AAATGTATTG TTTTTACATA GAAAGAAAAT 1651 GTTCCTTGTT GCTCTAGATG TTGGTGCTGT ATCCCTAATA CTTACGGGCC 1701 AAGCAAGAAG AAATTGTATA ATCTTTGTTG TTCAGAAGTT TCTAATAGAA 1751 TAAATAGGCC TGTAAGATGA ACTTGCCACT AGTAAATGTT ACTTTTAAGG 1801 ACATGAATAT GGAAGTATTA AATTATTCAA CAGATAAAAA AAAAA BLAST Results No BLAST result Medlme entries 98334590: Frabin, a novel FGDl-related actin filament-binding protein capable of changing cell shape and activating c-Jun N-termmal kinase. Peptide information for frame 3 ORF from 810 bp to 1373 bp; peptide length: 188 Category: similarity to known protein Classification: Cell structure/motility 1 MFSCFLCILS FSSLSNYSDL KKESAVNLNA PRTPGRHGLT TTPQQKLLSQ 51 HLPQRQGNDT DKTQGAQTCV ANGVMAAQNQ MECEEEKAAT LSSDTSIQAS 101 EPLLDTHIVN GERDETATAP AΞPTTDSCDG NAΞDSSYRTP GIGPVLPLEE 151 RGAETETKVQ ERENGESPLE LEQLDQHHEM KVEHETSS BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_72kl5, frame 3 TREMBL:AF038388_1 product: "actm-filament binding protein Frabin"; Rattus norvegicus actin-filament binding protein Frabin mRNA, complete eds., N = 1, Score = 428, P = 1.8e-39 >TREMBL:AF038388_1 product: "actm-filament binding protein Frabin"; Rattus norvegicus actm-filament binding protein Frabin mRNA, complete eds. Length = 766 HSPs: Score = 428 (64.2 bits), Expect = 1.8e-39, P = 1.8e-39 Identities = 90/174 (51%), Positives = 115/174 (66%) Query: 12 SSLSNYSDLKKESAVNLNAPRTPGRHGLTTTPQQKLLSQHLPQRQGNDTDKTQGAQTCVA 71 S LS+Y+D++K+S +NLN P+TP +HGLT+T QKL S PQ+Q D+D+ QG C+A Sbjct: 31 SVLSSYTDVQKDSTMNLNIPQTPRQHGLTSTTPQKLPSHKSPQKQEKDSDQNQGQHGCLA 90 Query: 72 NGVMAAQNQMECEEEKAATLSSDTSIQASEPLLDTHIVNGERDETATAPASPTTDSCDGN 131 NGV AAQ+QMECE EK A LS +T Q + D H++NG R+ET T AS T+S D N Sbjct: 91 NGVAAAQSQMECETEKEAALSPETDTQTAAASPDAHVLNGVRNETTTDSASSVTNSHDEN 150 Query: 132 ASDSSYRTPGIGPVLPLEERGAETETKVQERENGESPLELEQLDQHHEMKVEHE 185 A DSS RT G LP +E E ++QERENG S L LDQHHE+K +E Sbjct: 151 ACDSSCRTQGTDLGLPSKEGEPVIEAELQERENGLSTEGLNPLDQHHEVKETNE 204 Pedant information for DKFZphtes3_72kl5, frame 3 Report for DKFZphtes3_72kl5.3 [LENGTH] 188 [MW] 20388.32 [pi] 4.62 [HOMOL] TREMBL:AF038388_1 product: "actm-filament binding protein Frabin"; Rattus norvegicus actm-filament binding protein Frabin mRNA, complete eds. 2e-38 [KW] All_Alpha [KW] SIGNAL_PEPTIDE 16 [KW] LOW_COMPLEXITY 12.77 % SEQ MFΞCFLCILSFSSLSNYSDLKKESAVNLNAPRTPGRHGLTTTPQQKLLSQHLPQRQGNDT SEG . xxxxxxxxxxxxx PRD ccchhhhhcccccccccccccccccccccccccccccccccccchhhhhhhccccccccc SEQ DKTQGAQTCVANGVMAAQNQMECEEEKAATLSSDTSIQASEPLLDTHIVNGERDETATAP SEG xxxxx PRD ccccccceeecchhhhhhhhhhhhhhhhhhhccccceeecccccceeeeecccccccccc SEQ ASPTTDSCDGNASDSSYRTPGIGPVLPLEERGAETETKVQERENGESPLELEQLDQHHEM SEG xxxxx PRD ccccccccccccccccccccccccccccccccchhhhhhhhhcccccchhhhhhhhhhhh SEQ KVEHETSS SEG PRD hhhhhccc (No Prosite data available for DKFZphtes3_72kl5.3) (No Pfam data available for DKFZphtes3 72kl5.3) DKFZphtes3_72pl6 group: intracellular transport and trafficing DKFZphtes3_72pl6 encodes a novel 796 ammo acid protein with very strong similarity to Mus musculus maternal-embryonic 3 (Mem3) gene. Mem3 was isolated from a partial subtraction library of mouse unfertilized eggs and preimplantation embryos. Its transcript is abundant in the unfertilized egg and also actively transcribed from the newly formed zygotic genome. As Mem3, the novel protein is similar to yeast VPS (vacuolar protein sorting) 35. The null allele of VPS35 results in yeast in a differential defect in the sorting of vacuolar carboxypeptidase Y (CPY) , proteinase A (PrA) , proteinase B (PrB), and alkaline phosphatase (ALP). The new protein can find application in modulation the sorting of proteins into different compartments . strong similarity to mouse MEM3 and yeast VPS35 Sequenced by DKFZ Locus: /map="16pl3.3" Insert length: 2707 bp Poly A stretch at pos. 2697, no polyadenylation signal found 1 CTACGCGCGG GGCGGGTGCT GCTTGCTGCA GGCTCTGGGG AGTCGCCATG 51 CCTACAACAC AGCAGTCCCC TCAGGATGAG CAGGAAAAGC TCTTGGATGA 101 AGCCATACAG GCTGTGAAGG TCCAGTCATT CCAAATGAAG AGATGCCTGG 151 ACAAAAACAA GCTTATGGAT TCTCTAAAAC ATGCTTCTAA TATGCTTGGT 201 GAACTCCGGA CTTCTATGTT ATCACCAAAG AGTTACTATG AACTTTATAT 251 GGCCATTTCT GATGAACTGC ACTACTTGGA GGTCTACCTG ACAGATGAGT 301 TTGCTAAAGG AAGGAAAGTG GCAGATCTCT ACGAACTTGT ACAGTATGCT 351 GGAAACATTA TCCCAAGGCT TTACCTTTTG ATCACAGTTG GAGTTGTATA 401 TGTCAAGTCA TTTCCTCAGT CCAGGAAGGA TATTTTGAAA GATTTGGTAG 451 AAATGTGCCG TGGTGTGCAA CATCCCTTGA GGGGTCTGTT TCTTCGAAAT 501 TACCTTCTTC AGTGTACCAG AAATATCTTA CCTGATGAAG GAGAGCCAAC 551 AGATGAAGAA ACAACTGGTG ACATCAGTGA TTCCATGGAT TTTGTACTGC 601 TCAACTTTGC AGAAATGAAC AAGCTCTGGG TGCGAATGCA GCATCAGGGA 651 CATAGCCGAG ATAGAGAAAA AAGAGAACGA GAAAGACAAG AACTGAGAAT 701 TTTAGTGGGA ACAAATTTGG TGCGCCTCAG TCAGTTGGAA GGTGTAAATG 751 TGGAACGTTA CAAACAGATT GTTTTGACTG GCATATTGGA GCAAGTTGTA 801 AACTGTAGGG ATGCTTTGGC TCAAGAATAT CTCATGGAGT GTATTATTCA 851 GGTTTTCCCT GATGAATTTC ACCTCCAGAC TTTGAATCCT TTTCTTCGGG 901 CCTGTGCTGA GTTACACCAG AATGTAAATG TGAAGAACAT AATCATTGCT 951 TTAATTGATA GATTAGCTTT ATTTGCTCAC CGTGAAGATG GACCTGGAAT 1001 CCCAGCGGAT ATTAAACTTT TTGATATATT TTCACAGCAG GTGGCTACAG 1051 TGATACAGTC TAGACAAGAC ATGCCTTCAG AGGATGTTGT ATCTTTACAA 1101 GTCTCTCTGA TTAATCTTGC CATGAAATGT TACCCTGATC GTGTGGACTA 1151 TGTTGATAAA GTTCTAGAAA CAACAGTGGA GATATTCAAT AAGCTCAACC 1201 TTGAACATAT TGCTACCAGT AGTGCAGTTT CAAAGGAACT CACCAGACTT 1251 TTGAAAATAC CAGTTGACAC TTACAACAAT ATTTTAACAG TCTTGAAATT 1301 AAAACATTTT CACCCACTCT TTGAGTACTT TGACTACGAG TCCAGAAAGA 1351 GCATGAGTTG TTATGTGCTT AGTAATGTTC TGGATTATAA CACAGAAATT 1401 GTCTCTCAAG ACCAGGTGGA TTCCATAATG AATTTGGTAT CCACGTTGAT 1451 TCAAGATCAG CCAGATCAAC CTGTAGAAGA CCCTGATCCA GAAGATTTTG 1501 CTGATGAGCA GAGCCTTGTG GGCCGCTTCA TTCATCTGCT GCGCTCTGAG 1551 GACCCTGACC AGCAGTACTT GATTTTGAAC ACAGCACGAA AACATTTTGG 1601 AGCTGGTGGA AATCAGCGGA TTCGCTTCAC ACTGCCACCT TTGGTATTTG 1651 CAGCTTACCA GCTGGCTTTT CGATATAAAG AGAATTCTAA AGTGGATGAC 1701 AAATGGGAAA AGAAATGCCA GAAGATTTTT TCATTTGCCC ACCAGACTAT 1751 CAGTGCTTTG ATCAAAGCAG AGCTGGCAGA ATTGCCCTTA AGACTTTTTC 1801 TTCAAGGAGC ACTAGCTGCT GGGGAAATTG GTTTTGAAAA TCATGAGACA 1851 GTCGCATATG AATTCATGTC CCAGGCATTT TCTCTGTATG AAGATGAAAT 1901 CAGCGATTCC AAAGCACAGC TAGCTGCCAT CACCTTGATC ATTGGCACTT 1951 TTGAAAGGAT GAAGTGCTTC AGTGAAGAGA ATCATGAACC TCTGAGGACT 2001 CAGTGTGCCC TTGCTGCATC CAAACTTCTA AAGAAACCTG ATCAGGGCCG 2051 AGCTGTGAGC ACCTGTGCAC ATCTCTTCTG GTCTGGCAGA AACACGGACA 2101 AAAATGGGGA GGAGCTTCAC GGAGGCAAGA GGGTAATGGA GTGCCTAAAA 2151 AAAGCTCTAA AAATAGCAAA TCAGTGCATG GACCCCTCTC TACAAGTGCA 2201 GCTTTTTATA GAAATTCTGA ACAGATATAT CTATTTTTAT GAAAAGGAAA 2251 ATGATGCGGT AACAATTCAG GTTTTAAACC AGCTTATCCA AAAGATTCGA 2301 GAAGACCTCC CGAATCTTGA ATCCAGTGAA GAAACAGAGC AGATTAACAA 2351 ACATTTTCAT AACACACTGG AGCATTTGCG CTTGCGGCGG GAATCACCAG 2401 AATCCGAGGG GCCAATTTAT GAAGGTCTCA TCCTTTAAAA AGGAAATAGC 2451 TCACCATACT CCTTTCCATG TACATCCAGT GAGGGTTTTA TTACGCTAGG 2501 TTTCCCTTCC ATAGATTGTG CCTTTCAGAA ATGCTGAGGT AGGTTTCCCA 2551 TTTCTTACCT GTGATGTGTT TTACCCAGCA CCTCCGGACA CTCACCTTCA 2601 GGACCTTAAT AAAATTATTC ACTTGGTAAG TGTTCAAGTC TTTCTGATCA 2651 CCCCAAGTAG CATGACTGAT CTGCAATTTA AAATTCCTGT GATCTGTAAA 2701 AAAAAAA BLAST Results Entry AC007225 from database EMBLNEW: Homo sapiens chromosome 16 clone 480G7, WORKING DRAFT SEQUENCE, 3ε unordered pieces . Score = 1081, P = 2.8e-217, identities = 219/221 13 exons Entry HS015146 from database EMBL: human STS WI-8848. Score = 2033, P = 2.9e-87, identities = 425/436 Medline entries 96327632: Genetic mapping and embryonic expression of a novel, maternally transcribed gene Mem3. 97258867: Endosome to Golgi retrieval of the vacuolar protein sorting receptor, VpslOp, requires the function of the VPS29, VPS30, and VPS35 gene products. 92360909: Alternative pathways for the sorting of soluble vacuolar proteins in yeast: a vps35 null mutant missorts and secretes only a subset of vacuolar hydrolases. 10198044: Distinct Domains within Vps35p Mediate the Retrieval of Two Different Cargo Proteins from the Yeast Prevacuolar/Endosomal Compartment Peptide information for frame 3 ORF from 48 bp to 2435 bp; peptide length: 796 Category: strong similarity to known protein Classification: unset 1 MPTTQQSPQD EQEKLLDEAI QAVKVQSFQM KRCLDKNKLM DSLKHASNML 51 GELRTSMLSP KSYYELYMAI SDELHYLEVY LTDEFAKGRK VADLYELVQY 101 AGNIIPRLYL LITVGVVYVK SFPQSRKDIL KDLVEMCRGV QHPLRGLFLR 151 NYLLQCTRNI LPDEGEPTDE ETTGDISDSM DFVLLNFAEM NKLWVRMQHQ 201 GHSRDREKRE RERQELRILV GTNLVRLSQL EGVNVERYKQ IVLTGILEQV 251 VNCRDALAQE YLMECIIQVF PDEFHLQTLN PFLRACAELH QNVNVKNIII 301 ALIDRLALFA HREDGPGIPA DIKLFDIFSQ QVATVIQSRQ DMPSEDVVSL 351 QVSLINLAMK CYPDRVDYVD KVLETTVEIF NKLNLEHIAT SSAVSKELTR 401 LLKIPVDTYN NILTVLKLKH FHPLFEYFDY ESRKSMSCYV LSNVLDYNTE 451 IVSQDQVDSI MNLVSTLIQD QPDQPVEDPD PEDFADEQSL VGRFIHLLRS 501 EDPDQQYLIL NTARKHFGAG GNQRIRFTLP PLVFAAYQLA FRYKENSKVD 551 DKWEKKCQKI FSFAHQTISA LIKAELAELP LRLFLQGALA AGEIGFENHE 601 TVAYEFMSQA FSLYEDEISD SKAQLAAITL IIGTFERMKC FSEENHEPLR 651 TQCALAASKL LKKPDQGRAV STCAHLFWSG RNTDKNGEEL HGGKRVMECL 701 KKALKIANQC MDPSLQVQLF IEILNRYIYF YEKENDAVTI QVLNQLIQKI 751 REDLPNLESS EETEQINKHF HNTLEHLRLR RESPESEGPI YEGLIL BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_72pl6, frame 3 TREMBL :AF024504_3 gene: "A_TM017A05.7"; Arabidopsis thaliana BAC TM017A05., N = 2, Score = 927, P = 1.9e-162 PIR:S56936 vacuolar protem-sortmg protein VPS35 - yeast (Saccharomyces cerevisiae), N = 3, Score = 826, P = 1.5e-116 TREMBL:MM47024_1 gene: "Mem3"; product: "MEM3"; Mus musculus maternal-embryonic 3 (Mem3) mRNA, complete eds., N = 1, Score = 3376, P = 0 TREMBL:S42186_1 gene: "VPS35"; product: "Vps35p"; VPS35=vacuolar protein sorting [Saccharomyces cerevιsιae=yeast, Genomic, 3790 nt] , N = 3, Score = 813, P = 4.4e-115 >TREMBL:MM47024_1 gene: "Mem3"; product: "MEM3"; Mus musculus maternal-embryonic 3 (Mem3) mRNA, complete eds. Length = 754 HSPs: Score = 3376 (506.5 bits). Expect = O.Oe+00, P = O.Oe+00 Identities = 666/721 (92%), Positives = 682/721 (94%) Query: 78 EVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMC 137 +VYLTDEFAKG ++ADLYELVQY+GNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMC Sbjct: 34 KVYLTDEFAKGERLADLYELVQYSGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMC 93 Query: 138 RGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRM 197 RGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRM Sbjct: 94 RGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRM 153 Query: 198 QHQGHSRDREKRERERQELRILVGTNLVRLSQLEG-VNVERYKQIVLTGILEQVVNCRDA 256 QHQGHSRDREKRERERQELRILVGTNLV L+ + +QIVLTGILEQVVNCRDA Sbjct: 154 QHQGHSRDREKRERERQELRILVGTNLVALTLVSWRCKCGTLQQIVLTGILEQVVNCRDA 213 Query: 257 LAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGP 316 LAQE MECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHRE P Sbjct: 214 LAQEISMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREMEP 273 Query: 317 GIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETT 376 GIPA++KLFDIFSQQVATVIQSR+DMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETT Sbjct: 274 GIPAELKLFDIFSQQVATVIQSRRDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETT 333 Query: 377 VEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESR—K 434 VEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYES K Sbjct: 334 VEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESSPGK 393 Query: 435 SMΞCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRF 494 SMΞCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRF Sbjct: 394 SMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRF 453 Query: 495 IHLLRSEDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWE 554 IHLLRΞ+DPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSK + Sbjct: 454 IHLLRSDDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKWMTSGK 513 Query: 555 KKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLY 614 + ++ F HQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLY Sbjct: 514 RNARRYFHLPHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLY 573 Query: 615 EDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAAΞKLLKKPDQGRAVSTCA 674 EDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRT+CALAASKLLKKPDQ C Sbjct: 574 EDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTECALAASKLLKKPDQAEREHMCT 633 Query: 675 HLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKE 734 L WSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKE Sbjct: 634 SL-WSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKE 692 Query: 735 NDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRLRRESPESEGPIYEGL 794 NDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLR RRESPESEGPIYEGL Sbjct: 693 NDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRTRRESPESEGPIYEGL 752 Query: 795 IL 796 IL Sbjct: 753 IL 754 Pedant information for DKFZphtes3_72pl6, frame 3 Report for DKFZphtes3_72pl6.3 [LENGTH] 796 [MW] 91723.67 [pi] 5.32 [HOMOL] TREMBL:MM47024_1 gene: "Mem3"; product: "MEM3"; Mus musculus maternal-embryonic 3 (Mem3) mRNA, complete eds. 0.0 [FUNCAT] 30.25 vacuolar and lysosomal organization [S. cerevisiae, YJL154c] le-110 [FUNCAT] 08.13 vacuolar transport [S. cerevisiae, YJL154c] le-110 [FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YJL154c] le-110 [FUNCAT] 30.22 endosomal organization [S. cerevisiae, YJL154c] le-110 [FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YJL154c] le-110 [FUNCAT] 30.08 organization of golgi [S. cerevisiae, YJL154c] le-110 [FUNCAT] 09.07 biogenesis of endoplasmatic reticulum [S. cerevisiae, YJL154c] le-110 [BLOCKS] BL01092Q [PIRKW] yeast vacuole le-108 [PIRKW] membrane protein le-108 [KW] TRANSMEMBRANE 1 [KW] LOW COMPLEXITY 5.40 % SEQ MPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDSLKHASNMLGELRTSMLSP SEG PRD cccccccccchhhhhhhhhhhhhhhhhhhhhhhhcccchhhhhhhhhhhhhhhhhhcccc MEM SEQ KSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVK SEG PRD cceeeeehhhhhhhhhhhhhhhhhhhchhhhhhhhhhhhhhcccccccceeeeeceeeee MEM MMMMMMMMMMMMMM SEQ SFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSM SEG xxxxxxxxxxxxxx PRD ecccchhhhhhhhhhhhhhccccchhhhhhhhhhhhhhhcccccccccccccccccccch MEM MMMMMMMMMM SEQ DFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQ SEG xxxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhhcccchhhhhhhhhhhhhhhhhccchhhhhhhhccchhhhhh MEM SEQ IVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIII SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccchhhhhhhhhhhhccccchhhhhh MEM SEQ ALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMK SEG PRD hhhhhhhhhhhhcccccccccchhhhhhhhhhhhhhhhccccccccchhhhhhhhhhhhh MEM SEQ CYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKH SEG PRD cccccccchhhhhhhhhhhhhccchhhhhhccchhhhhhhhhccccccchhhhhhhhhhh MEM SEQ FHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPD SEG xxxxxxxxxxxx PRD hhhheeecccchhhhhhhhhhhhccccceeehhhhhhhhhhhhhhhhhhccccccccccc MEM SEQ PEDFADEQSLVGRFIHLLRSEDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLA SEG xxx PRD ccccchhhhhhhhhhhhhhccccchhhhhhhhhhhhhcccccceeeeeccchhhhhhhhh MEM SEQ FRYKENSKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHE SEG PRD hhhhhcccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccc MEM SEQ TVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAASKL SEG PRD eeeeehhhhhhhhhhhhhhchhhhhhhhhhhhhhhhhhhhcccccccchhhhhhhhhhhh MEM SEQ LKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLF SEG PRD hhcccceeeeecccccccccccccccccccccchhhhhhhhhhhhhhhhhhchhhhhhhh MEM SEQ IEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRLR SEG PRD hhhhhhhhhhhccccceeeeehhhhhhhhhhhhhhhhhccccchhhhhhhhhhhhhhhhh MEM SEQ RESPESEGPIYEGLIL SEG PRD hhcccccccceeeccc MEM (No Prosite data available for DKFZphtes3_72pl6 . 3 ) (No Pfam data available for DKFZphtes3_72pl 6. 3 ) DKFZphtes3_7b22 group: cell structure and motility DKFZphtes3_7b22 encodes a novel 443 amino acid protein with weak similarity to paramyosins. The novel protein is related to paramyosin, a major structural component of thick filaments and invertebrate muscle. Paramyosins are promising antigens for immunization against several parasites, such as Schistosoma mansom . The new protein can find application in modulating cell adhesion/motility and membrane/cyto skeleton structure and dynamic. similarity to paramyosins complete cDNA, complete eds, few EST hits Sequenced by BMFZ Locus: /map="3" Insert length: 2291 bp Poly A stretch at pos. 2241, polyadenylation signal at pos. 2213 1 GGAAGAAAGG CTAGCGGGCG TTGGCCGTAT GTGGGTGTCT TGAGGCAGTT 51 TTTCAGTTCT TTCATTTACC AAAGTGACAT GCACCTACTA GGTGCCAGGT 101 GTTTAGACGT ACATACAACC CTCTGCAAAA TCTTTCAGTG TAGTCCTCTG 151 TATGAAAAGT TTCCAGCCAA GAATTGCCAC TGCACCTGAG ATAAGGGGGA 201 TCCTGGCCAT TAAGGAAACC TTGCCTTCGA AACTGAGCCG TGAGGAACTA 251 TACAAAATGG GAAATTGGGA CAAATCCCAG TGGCTCATGA CACTAAGAAG 301 TAAAATTACG AACTCACTGA GCTGGAAGTC ATTCAACGGG AATTGAATAG 351 GTAACTGCAC TTTTGTGAGA TTATAAATAT ACCACGGAGG GTAACGAAGC 401 TACAGAAGAA TGGAAGAAGA CAGCCTGGAA GACTCAAACC TTCCTCCAAA 451 AGTTTGGCAT TCTGAGATGA CGGTGTCAGT GACAGGCGAA CCACCTAGTA 501 CCGTAGAAGA AGAAGGAATA CCTAAAGAAA CAGACATAGA AATCATCCCA 551 GAAATCCCGG AAACTCTAGA GCCACTGTCC CTTCCAGATG TGCTGAGGAT 601 CTCGGCAGTT CTGGAGGACA CCACAGACCA GCTCTCTATT CTGAACTACA 651 TCATGCCCGT TCAGTACGAA GGGAGACAGA GCATCTGCGT GAAAAGCAGA 701 GAAATGAATC TAGAAGGAAC GAATCTAGAC AAACTTCCAA TGGCCTCAAC 751 AATCACAAAA ATACCCAGTC CGTTAATAAC TGAGGAAGGA CCCAACTTGC 801 CAGAAATCAG ACACAGAGGC CGGTTCGCTG TGGAGTTTAA CAAAATGCAG 851 GATCTTGTCT TCAAAAAACC TACAAGGCAG ACCATCATGA CTACGGAGAC 901 ACTGAAGAAA ATTCAGATTG ATAGGCAGTT TTTCAGCGAT GTGATTGCAG 951 ATACCATTAA GGAGTTGCAA GATTCGGCCA CTTACAACAG TCTCCTGCAA 1001 GCTTTGAGCA AAGAGAGGGA AAACAAAATG CATTTCTATG ACATCATTGC 1051 CAGGGAGGAA AAAGGAAGAA AACAGATAAT ATCACTTCAA AAACAGCTAA 1101 TTAATGTCAA AAAGGAATGG CAATTTGAAG TCCAGAGTCA GAATGAGTAT 1151 ATTGCTAACC TCAAGGACCA ACTGCAAGAG ATGAAGGCAA AATCCAACTT 1201 GGAGAATCGC TACATGAAAA CCAATACCGA GCTGCAGATT GCCCAGACCC 1251 AGAAAAAGTG TAACAGAACA GAGGAACTCT TGGTGGAAGA GATTGAGAAA 1301 CTCAGGATGA AAACCGAAGA AGAGGCCCGG ACTCATACAG AGATTGAAAT 1351 GTTCCTTAGA AAGGAGCAGC AGAAACTTGA GGAGAGGCTG GAGTTCTGGA 1401 TGGAGAAATA CGATAAGGAC ACAGAAATGA AACAGAATGA ACTAAATGCT 1451 CTCAAAGCCA CAAAGGCCAG TGACTTAGCA CACCTTCAAG ACCTGGCAAA 1501 GATGATAAGA GAGTATGAAC AGGTCATCAT TGAAGATCGT ATAGAAAAGG 1551 AGAGGAGCAA GAAGAAGGTA AAACAGGATC TCTTGGAATT AAAGAGCGTT 1601 ATAAAGCTCC AGGCCTGGTG GCGAGGCACT ATGATACGGA GAGAAATTGG 1651 TGGTTTCAAG ATGCCTAAAG ACAAAGTTGA TAGCAAGGAT TCAAAAGGCA 1701 AAGGTAAAGG CAAGGATAAG AGGAGAGGCA AGAAGAAGTG ACCAAGTTCT 1751 CTTTTGTGTT TTCTGCTGGT ATTCTGGAGG TGGGAAGGAC TTGGAGAGTT 1801 AAGAAACACC TGGTACCTCA AAGATGACTC ATCTACAGGT TGTTTCCTAT 1851 TGAGACTTTC CCAGGGAAGC CTGATTTCAC TTTGCCTGTT AATTTCACTC 1901 TGCCTGTTAG GTGGGTTTTC AAACCCTGAT TTAGGATTAC ACCATTGACT 1951 TAGGGCTTCC TCATACCTTG CTGGGAAGAA GTTTCTAGTA GTCCTGTGAA 2001 GATTCATTCT TCTTGCTCTT TCTCAGCAGA ACAAAGGAGT TCACTGGCTT 2051 AGCTACAGTG ACGCATTGAA ACTTGAGTAA TTCCTGTAAT GTCAGATTTT 2101 GATTTTACCC AATTTGTCTG TAGTGAAAAA ACTCTTATGA GCAAAAGTAT 2151 TCAGTAGGAA TTACAATATG ATGTTATTAG CTGTCCAGCA TAATATATAC 2201 ACAGCAAAGT TTTAATAAAT GTTGGTTCCT GCCTGCCTTT TAAAAAAAAA 2251 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA A BLAST Results Entry G36731 from database EMBL: SHGC-52923 Human Homo sapiens STS cDNA. Score = 2262, P = 1.3e-97, identities = 462/468 Medline entries No Medlme entry Peptide information for frame 2 ORF from 410 bp to 1738 bp; peptide length: 443 Category: similarity to known protein 1 MEEDSLEDSN LPPKVWHSEM TVSVTGEPPS TVEEEGIPKE TDIEIIPEIP 51 ETLEPLSLPD VLRISAVLED TTDQLSILNY IMPVQYEGRQ SICVKSREMN 101 LEGTNLDKLP MASTITKIPS PLITEEGPNL PEIRHRGRFA VEFNKMQDLV 151 FKKPTRQTIM TTETLKKIQI DRQFFSDVIA DTIKELQDSA TYNSLLQALS 201 KERENKMHFY DIIAREEKGR KQIISLQKQL INVKKEWQFE VQSQNEYIAN 251 LKDQLQEMKA KSNLENRYMK TNTELQIAQT QKKCNRTEEL LVEEIEKLRM 301 KTEEEARTHT EIEMFLRKEQ QKLEERLEFW MEKYDKDTEM KQNELNALKA 351 TKASDLAHLQ DLAKMIREYE QVIIEDRIEK ERSKKKVKQD LLELKΞVIKL 401 QAWWRGTMIR REIGGFKMPK DKVDSKDSKG KGKGKDKRRG KKK BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_7b22, frame 2 SWISSPROT :MYSP_BRUMA PARAMYOSIN., N = 1, Score = 158, P = 5.8e-08 PIR:A44972 paramyosin - nematode (Dirofilaria lmmitis) (fragment), N = 1, Score = 157, P = 7. le-08 SWISSPROT:MYSP_ONCVO PARAMYOSIN., N = 1, Score = 157, P = 7.4e-08 PIR:S52537 emm L 15 protein - Streptococcus pyogenes, N = 1, Score = 151, P = 8.6e-08 >SWISSPROT:MYSP_BRUMA PARAMYOSIN. Length = 880 HSPs: Score = 158 (23.7 bits), Expect = 5.8e-08, P = 5.8e-08 Identities = 66/259 (25%), Positives = 125/259 (48%) Query: 142 EFNKMQDLVFKKPTRQTIMTTETLKKIQIDRQFFSDVIADTIKELQDSATYNSLLQALSK 201 + K + L K R T E K++ + +D +A + LQ A N LL+ + Sbjct: 169 QLKKDKHLAEKAAERFEAQTVELSNKVEDLNRHVND-LAQQRQRLQ—AENNDLLKEIHD 225 Query: 202 ER ENKMHF-YDIIAREEKGRKQIISLQKQLINVKKEWQFEVQSQNEYIANLKDQLQE 257 ++ +N H Y + + E+ R+++ +++ ++ + +VQ + + + D+ E Sbjct: 226 QKVQLDNLQHVKYQLAQQLEEARRRLEDAERERSQLQAQLH-QVQLELDSVRTALDE—E 282 Query: 258 MKAKSNLENRYMKTNTELQIAQTQKKCNRTEELLVEEIEKLRMKT-EEEARTHTEIEMFL 316 A++ E++ NTE I Q + K + L EE+E LR K +++A +IE+ L Sbjct: 283 SAARAEAEHKLALANTE—ITQWKSKFDAEVALHHEEVEDLRKKMLQKQAEYEEQIEIML 340 Query: 317 RKEQQ—KLEERLEFWMEKYDKDTEMKQNELNALKATKASDLAHLQDLAKMIREYEQVII 374 +K Q K + RL+ +E D E QN + L+ K + L K + E + I Sbjct: 341 QKISQLEKAKSRLQSEVEVLIVDLEKAQNTIAILERAK EQLEKTVNELKVRID 393 Query: 375 EDRIEKERSKKKVKQDLLELKSVIKL 400 E +E E ++++ + L EL+ + L Sbjct: 394 ELTVELEAAQREARAALAELQKLKNL 419 Score = 118 (17.7 bits), Expect = 1.3e-03, P = 1.3e-03 Identities = 54/231 (23%), Positives = 108/231 (46%) Query: 181 DTIKELQDSATYNSLLQ ALSKERENKMHFYDIIAREEKG-RKQIISLQKQLINVKK 235 D +KE+ D LQ L+++ E + RE + Q+ +Q +L +V+ Sbjct: 218 DLLKEIHDQKVQLDNLQHVKYQLAQQLEEARRRLEDAERERSQLQAQLHQVQLELDSVRT 277 Query: 236 EWQFE—VQSQNEY-IANLKDQLQEMKAKSNLENRYMKTNTE-LQIAQTQKKCNRTEELL 291 E +++ E+ +A ++ + K+K + E E L+ QK+ E++ Sbjct: 278 ALDEESAARAEAEHKLALANTEITQWKSKFDAEVALHHEEVEDLRKKMLQKQAEYEEQIE 337 Query: 292 VEEIEKLRMKTEEEARTHTEIEMF LRKEQQKLE—ERLEFWMEKYDKDTEMKQNELN 346 + ++K+ + ++R +E+E+ L K Q + ER + +EK + +++ +EL Sbjct: 338 IM-LQKISQLEKAKΞRLQSEVEVLIVDLEKAQNTIAILERAKEQLEKTVNELKVRIDELT 396 Query: 347 A-LKATKASDLAHLQDLAKMIREYEQVIIEDRIEKERSKKKVKQDLLELKSVI 398 L+A + A L +L K+ YE+ + E + R KK++ DL E K + Sbjct: 397 VELEAAQREARAALAELQKLKNLYEKAV-EQKEALARENKKLQDDLHEAKEAL 448 Score . = 107 (16.1 bits), Expect = 2.1e-02, P = 2.1e-02 Identities : = 49/279 (17%), Positives = 124/279 (44%) Query: 123 ITEEGPNLPEIRHRGRFAV-EFNKMQDLVFKKPTRQTIMTTETLKKIQIDRQFFSDVIAD 181 I E L + R A+ E K+++L K ++ + E KK+Q D + +AD Sbjct: 392 IDELTVELEAAQREARAALAELQKLKNLYEKAVEQKEALAREN-KKLQDDLHEAKEALAD 450 Query: 182 TIKELQDSATYNSLLQALSKERENKMHFYDIIAREEKGRKQ—IISLQKQLINVKKEWQF 239 ++L + N+ L +E + + + R+ + R Q + LQ+ I +++ Q Sbjct: 451 ANRKLHELDLENARLAGEIRELQTALKESEAARRDAENRAQRALAELQQLRIEMERRLQE 510 Query: 240 EVQSQNEYIANLKDQLQEMKAKSNLENRYMKTNTELQIAQTQKKCNRTE-ELLVEEIEKL 298 + + N++ ++ + A L + + E+ + + + E E+ V+ + + Sbjct: 511 KEEEMEALRKNMQFEIDRLTAA—LADAEARMKAEISRLKKKYQAEIAELEMTVDNLNRA 568 Query: 299 RMKTEEEARTHTEIEMFLRKEQQKLEERLEFWMEKYDKDTEMKQNELNALKATKASDLAH 358 ++ ++ + +E L+ + + +L+ +++Y + Q +++AL A + + Sbjct: 569 NIEAQKTIKKQSEQLKILQASLEDTQRQLQQTLDQY ALAQRKVSALSA-ELEECKV 623 Query: 359 LQDLAKMIREYEQVIIEDRIEKERSKKKVKQDLLELKSVIKLQ 401 D A R+ ++ +E+ + V +L +K+ ++ + Sbjct: 624 ALDNAIRARKQAEIDLEEANGRITDLVSVNNNLTAIKNKLETE 666 Pedant information for DKFZphtes3_7b22, frame 2 Report for DKFZphtes3_7b22.2 [LENGTH] 443 [MW] 51917.95 [pl] 6.18 [HOMOL] PIR:S28589 trichohyalm - rabbit 2e-08 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YDL058w] 7e-07 [FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YDL058w] 7e-07 [FUNCAT] 1 genome replication, transcription, recombination and repair [M. jannaschii, MJ1322] 5e-06 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YPR141c] le-05 [FUNCAT] 03.13 meiosis [S. cerevisiae, YPR141c] le-05 [FUNCAT] 11.01 stress response [S. cerevisiae, YPR141c] le-05 [FUNCAT] 03.07 pheromone response, matmg-type determination, sex-specific proteins [S. cerevisiae, YPR141c] le-05 [FUNCAT] 08.22 cytoskeleton-dependent transport [S. cerevisiae, YPR141c] le-05 [FUNCAT] 09.10 nuclear biogenesis [S. cerevisiae, YPR141c] le-05 [FUNCAT] 30.05 organization of centrosome [S. cerevisiae, YPR141c) le-05 [FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YPR141c] le-05 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YOR216c] 3e-05 [FUNCAT] 11.04 dna repair (direct repair, base excision repair and nucleotide excision repair) [S. cerevisiae, YKR095w] 6e-05 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YKR095w] 6e-05 [FUNCAT] 30.02 organization of plasma membrane [S. cerevisiae, YER008c] le-04 [FUNCAT] 08.16 extracellular transport [S. cerevisiae, YER008c] le-04 [FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YER008c] le-04 [FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YDR356w] 2e-04 [FUNCAT] 08.01 nuclear transport [S. cerevisiae, YDL207w] 4e-04 [FUNCAT] 04.07 rna transport [S. cerevisiae, YDL207w] 4e-04 [FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylati an, farnesylation and processing) [S. cerevisiae, YKL201c] 5e-04 [EC] 3.6.1.32 Myosin ATPase 3e-08 [PIRKW] phosphotransferase 6e-06 [PIRKW] citrulline 8e-06 [PIRKW] tandem repeat le-07 [PIRKW] heart 6e-06 [PIRKW] polymorphism 4e-06 [PIRKW] ser ne/threomne-specific protein kinase 6e-06 [PIRKW] DNA binding 8e-08 [PIRKW] muscle contraction le-07 [PIRKW] actm binding 3e-08 [PIRKW] ATP 3e-08 [PIRKW] thick filament le-07 [PIRKW] phosphoprotein 3e-08 [PIRKW] glycoprotein 4e-06 [PIRKW] skeletal muscle le-07 [PIRKW] calcium binding 8e-06 [PIRKW] alternative splicing 3e-08 [PIRKW] coiled coil 3e-08 [PIRKW] P-loop 3e-08 [PIRKW] heptad repeat 4e-06 [PIRKW] methylated amino acid 3e-08 [PIRKW] basement membrane 4e-06 [PIRKW] cardiac muscle 6e-06 [PIRKW] extracellular matrix 4e-06 [PIRKW] hydrolase 3e-08 [PIRKW] membrane protein 4e-06 [PIRKW] EF hand 8e-06 [PIRKW] cytoskeleton 8e-06 [PIRKW] hair 8e-06 [SUPFAM] myosin heavy chain 3e-08 [SUPFAM] unassigned Ser/Thr or Tyr-specific protein kinases 6e-06 [SUPFAM] calmodulin repeat homology 8e-06 [SUPFAM] myosin motor domain homology 3e-08 [SUPFAM] trichohyalm 8e-06 [SUPFAM] protein kinase homology 6e-06 [PROSITE] AMIDATION 2 [PROSITE] CAMP_PHOSPHO_SITE 1 [PROSITE) CK2_PHOSPHO_SITE 12 [PROSITE] TYR_PHOSPHO_SITE 2 [PROSITE] PKC_PHOSPHO_SITE 4 [PROSITE] ASN_GLYCOSYLATION [KW] All_Alpha [KW] LOW COMPLEXITY 10.61 % SEQ MEEDSLEDSNLPPKVWHSEMTVSVTGEPPSTVEEEGIPKETDIEIIPEIPETLEPLSLPD SEG xxxxxxxxxxxxxxxxxxxxxxx . PRD cccccccccccccccccceeeeeccccccceeeeecccccceeeeeeccccccccccccc SEQ VLRISAVLEDTTDQLSILNYIMPVQYEGRQSICVKSREMNLEGTNLDKLPMASTITKIPS SEG PRD chhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh SEQ PLITEEGPNLPEIRHRGRFAVEFNKMQDLVFKKPTRQTIMTTETLKKIQIDRQFFSDVIA SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh SEQ DTIKELQDSATYNSLLQALSKERENKMHFYDIIAREEKGRKQIISLQKQLINVKKEWQFE SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh SEQ VQSQNEYIANLKDQLQEMKAKΞNLENRYMKTNTELQIAQTQKKCNRTEELLVEEIEKLRM SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh SEQ KTEEEARTHTEIEMFLRKEQQKLEERLEFWMEKYDKDTEMKQNELNALKATKASDLAHLQ SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh SEQ DLAKMIREYEQVIIEDRIEKERSKKKVKQDLLELKSVIKLQAWWRGTMIRREIGGFKMPK SEG x PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccccc SEQ DKVDSKDSKGKGKGKDKRRGKKK SEG xxxxxxxxxxxxxxxxxxxxxxx PRD CCCCCCCCCCCCCCCCCCCCCCC Prosite for DKFZphtes3_7b22.2 PS00001 285- ->289 ASN GLYCOSYLATION PDOC00001 PS00004 152- ->156 CAMP PHOSPHO SITE PDOC00004 PS00005 164- ->167 PKC PHOSPHO SITE PDOC00005 PS00005 182- ->185 PKC PHOSPHO SITE PDOC00005 PS00005 280- ->283 PKC PHOSPHO SITE PDOC00005 PS00005 383- ->386 PKC PHOSPHO SITE PDOC00005 PS00006 5->9 CK2 PHOSPHO SITE PDOC00006 PS00006 30->34 CK2 PHOSPHO SITE PDOC00006 PS00006 41->45 CK2 PHOSPHO SITE PDOC00006 PS00006 57->61 CK2 PHOSPHO " " SITE PDOC00006 PS00006 104->108 CK2 PHOSPHO " SITE PDOC00006 PS00006 182->186 CK2 PHOSPHO SITE PDOC00006 PS00006 243->247 CK2 PHOSPHO " " SITE PDOC00006 PS00006 262->266 CK2 PHOSPHO " " SITE PDOC00006 PΞ00006 271->275 CK2 PHOSPHO " " SITE PDOC00006 PS00006 302->306 CK2 PHOSPHO " " SITE PDOC00006 PS00006 308->312 CK2 PHOSPHO " " SITE PDOC00006 PS00006 310->314 CK2 PHOSPHO SITE PDOC00006 PS00007 261->269 TYR PHOSPHO " " SITE PDOC00007 PS00007 184->193 TYR PHOSPHO " " SITE PDOC00007 PS00009 218->222 AMIDATION PDOC00009 PS00009 439->443 AMIDATION PDOC00009 (No Pfam data available for DKFZphtes3_7b22.2 ) DKFZphtes3_7dl7 group: testes derived DKFZphtes3_7dl7 encodes a novel 633 amino acid protein with weak similarity to human KIAA0454. Pfam predicts a TNFR/NGFR cysteme-rich region. No informative BLAST results; No predictive prosite or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . similarity to KIAA0454 complete cDNA, complete eds, EST hits Sequenced by BMFZ Locus : unknown Insert length: 3608 bp Poly A stretch at pos. 3587, polyadenylation signal at pos. 3570 1 GGGAAGTTAC GGCGAAGTCC ACCCAGCGTT TCTCAGGCAA TCTGAAGGCA 51 AATCCTGTTT AGACCCAGGC GAAGGTTCCT GGTGACCCAG GCTCTCACCA 101 GCCAATTGTC CCTTGCCGTC CTCCTGAGGG TATCTGGAGC TTCAGTGCTG 151 TGTGCTCTTG GCCTCCACAC TGGGGATGCC ACTGACTCCC ACTGTCCAGG 201 GCTTCCAGTG GACTCTCCGA GGCCCTGATG TAGAAACTTC CCCATTCGGT 251 GCACCAAGAG CAGCCTCACA TGGTGTGGGC CGACATCAAG AGCTGCGAGA 301 TCCAACAGTC CCTGGCCCCA CCTCTTCTGC CACAAACGTC AGCATGGTGG 351 TATCTGCCGG CCCTTGGTCC GGTGAGAAGG CAGAGATGAA CATTCTAGAA 401 ATCAACAAGA AATCGCGCCC CCAGCTGGCA GAGAACAAAC AGCAGTTCAG 451 AAACCTCAAA CAGAAATGTC TTGTAACTCA AGTGGCCTAC TTCCTGGCCA 501 ACCGGCAAAA TAATTACGAC TATGAAGACT GCAAAGACCT CATAAAATCT 551 ATGCTGAGGG ATGAGCGGCT GCTCACAGAA GAGAAGCTTG CAGAGGAGCT 601 CGGGCAAGCT GAGGAGCTCA GGCAATATAA AGTCCTGGTT CACTCTCAGG 651 AACGAGAGCT GACCCAGTTA AGGGAGAAGT TACAGGAAGG GAGAGATGCC 701 TCCCGCTCAT TGAATCAGCA TCTCCAGGCC CTCCTCACTC CGGATGAGCC 751 GGACAACTCC CAGGGACGGG ACCTCCGAGA ACAGCTGGCT GAGGGATGTA 801 GGCTGGCACA GCACCTCGTC CAAAAGCTCA GCCCAGAAAA TGATGACGAT 851 GAGGATGAAG ATGTTAAAGT TGAGGAGGCT GAGAAAGTAC AGGAATTATA 901 TGCCCCCAGG GAGGTGCAGA AGGCTGAAGA AAAGGAAGTC CCTGAGGACT 951 CACTGGAGGA GTGTGCCATC ACTTGTTCAA ATAGCCACCA CCCTTGTGAG 1001 TCCAACCAGC CTTACGGGAA CACCAGAATC ACATTTGAGG AAGACCAAGT 1051 CGACTCAACT CTCATTGACT CATCCTCTCA TGATGAATGG TTGGATGCTG 1101 TATGCATTAT CCCAGAAAAT GAAAGTGATC ATGAGCAAGA GGAAGAAAAA 1151 GGGCCAGTGT CTCCCAGGAA TCTGCAGGAG TCTGAAGAGG AGGAAGCCCC 1201 CCAGGAGTCC TGGGATGAAG GTGATTGGAC TCTCTCAATT CCTCCTGACA 1251 TGTCTGCCTC ATACCAGTCT GACAGGAGCA CCTTTCACTC AGTAGAGGAA 1301 CAGCAAGTCG GCTTGGCTCT TGACATAGGC AGACATTGGT GTGATCAAGT 1351 GAAAAAGGAG GACCAAGAGG CCACAAGTCC CAGGCTCAGC AGGGAGCTGC 1401 TGGATGAGAA AGAGCCTGAA GTCTTGCAGG ACTCACTGGA TAGATTTTAT 1451 TCAACTCCTT TTGAGTACCT GGAACTGCCT GACTTATGCC AGCCCTACAG 1501 AAGTGACTTT TACTCATTGC AGGAACAACA CCTTGGCTTG GCTCTTGACT 1551 TGGACAGAAT GAAAAAGGAC CAAGAAGAGG AAGAAGACCA AGGCCCACCA 1601 TGCCCCAGGC TCAGCAGAGA GCTGCCGGAG GTAGTAGAGC CTGAGGACTT 1651 GCAGGACTCA CTGGATAGAT GGTATTCGAC TCCTTTCAGT TATCCAGAAC 1701 TGCCTGATTC ATGCCAGCCC TACGGAAGTT GCTTTTACTC ATTGGAGGAA 1751 GAACACGTTG GCTTTTCTCT TGACGTGGAT GAAATTGAAA AGTACCAAGA 1801 AGGGGAAGAA GATCAAAAGC CACCATGCCC CAGGCTCAAC GAGGTGCTGA 1851 TGGAAGCAGA AGAGCCTGAA GTCTTGCAGG ACTCACTGGA TAGATGTTAT 1901 TCGACTACTT CAACTTACTT TCAACTACAT GCCTCATTCC AGCAGTACAG 1951 AAGTGCCTTT TACTCATTTG AGGAACAGGA CGTCAGCTTG GCCCTTGACG 2001 TGGACAATAG GTTTTTTACT TTGACAGTGA TAAGGCACCA CCTGGCCTTC 2051 CAGATGGGAG TCATATTCCC ACACTAAGCA GCCCTTACTA AGCTGAGAGA 2101 TGTCATTGCT GCAGGCAGGA CCTATAGGCA CATGTAGGTT TGAATGAAAC 2151 TGTAGTTCCC TTTGGAAGCC CAGTCATAGG ATGGGAAAGT GGGCATGGCT 2201 CTATTCCTAT TCTCAGACCA TGCCAGTGGC CACCTGTGCT CAGTCTGAAG 2251 ACGTTGGACC CAAGTTAGGT GTGACACGTT CACACGACTA TGTAGCACAT 2301 GCCGGGAGTG ATCTGCCAGA CATTCTAATT TGAACCAGAT ATCTCTGGGT 2351 AGCTACAAAG TTCCTCAGGG GTTTCATTTT GCAGGCATGT CTCTGAGCTT 2401 CTATACCTGC TCAAGGTCAG TGTCATCTTT GTGTTTAGCT CATCCAAAGG 2451 TGTTACCCTG GTTTCATTGA ACCTAACCCC ATTCTTTGTA TCTTCAGTGT 2501 TGGTTTGTTT TAGCTGATCC ATCTGTAACA CAGGAGGGAT CCTTGGCTGA 2551 GGATTGTATT TCAGAACCAC TGACTGCTCT TGACAGTTGT TAACCCACTA 2601 GGCTCCTTTG AGTAGAGAAG CCATAGTCCT TCAGCCTCCA ATTGATATCA 2651 ATACTTAGGA AGACCACAGC TAGACGGACA AACAGCATTG GGAGGCCTTA 2701 GTCCTGCTCC TTTCAATTCC ATCCTGTAAA GAACAGGAGT CAGGAGCCGC 2751 TGGCAAGAGA CAGCATGTCA CCTGGGACTC TGCCAGTGCA GAATATGAAC 2801 AATGCCATGT TCTTGCAGAA AATGCTTAGC CTGAGTTTCA TAGGAGGTAA 2851 TCACCAGACA ACTGCAGAAT GTAGAACACT GAGCAGGACA ACTGACCTGT 2901 CTCCTTCACA CAGTCCACGT CACCACGAAT CACACAACAA AAAGGAGGAG 2951 AGATATTTTG GGTTCAGAAG AAGTAAATGA TAATGTAGCT ACATTTCTTT 3001 AGTTATTTTG AACCCCAAAT ATTTCCTCAT CTTTTTGTTG TTGTCATTGA 3051 TTTTGGTGAC ATGGACTTGT TTGTAGAGGA CAGGTCAGCT GTCTGGCTCA 3101 ATGGTCTACA TTCTGAAGTT GTCTGAAAAT GTCTTCATGA TTAAATTCAG 3151 CCTAAACGTT TCATCAAGAA CACTACAGAG TCGATACTGT GAGTTTCCAA 3201 CCTCAGCCCA TCTGTGGGCA GAGAAGGTCT AGTTTGTCCA TCAGCATTAT 3251 CATGATATCA GGACTGGTTA CTTGGTTAAG GAGGGGTCTA GGAGATCTGT 3301 CCCTTTTAGA GACACCTTAC TTATGATGAA GTATTTGGGA GAGTGGTTTT 3351 TCAAAGTAGA AATGTCCTGT ATTCCAGTGA TCATCCTCTA AACGTTTTAT 3401 CATTTATTAA TCATCCCTGC CTGTGTCTAT TATTATATTC ATATCTCTAC 3451 GCTGGAAATT TGCTGCCTCA ATGTTTACTG TGCCTTTGTT TTTGCTAGTG 3501 TGTGTTGTTG AAAAAAAAAC ATTCTCTGCC TGAGTTTTAA TTTTTGTCCA 3551 AAGTTATTTT AATCTATACA ATTAAAAACT TTTGCCTATC AAAAAAAAAA 3601 AAAAAAAA BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 2 ORF from 176 bp to 2074 bp; peptide length: 633 Category: similarity to known protein 1 MPLTPTVQGF QWTLRGPDVE TSPFGAPRAA SHGVGRHQEL RDPTVPGPTS 51 SATNVSMVVS AGPWSGEKAE MNILEINKKS RPQLAENKQQ FRNLKQKCLV 101 TQVAYFLANR QNNYDYEDCK DLIKSMLRDE RLLTEEKLAE ELGQAEELRQ 151 YKVLVHSQER ELTQLREKLQ EGRDASRSLN QHLQALLTPD EPDNSQGRDL 201 REQLAEGCRL AQHLVQKLSP ENDDDEDEDV KVEEAEKVQE LYAPREVQKA 251 EEKEVPEDSL EECAITCSNS HHPCESNQPY GNTRITFEED QVDSTLIDSS 301 SHDEWLDAVC IIPENESDHE QEEEKGPVSP RNLQESEEEE APQESWDEGD 351 WTLSIPPDMS ASYQSDRSTF HSVEEQQVGL ALDIGRHWCD QVKKEDQEAT 401 SPRLSRELLD EKEPEVLQDS LDRFYSTPFE YLELPDLCQP YRSDFYSLQE 451 QHLGLALDLD RMKKDQEEEE DQGPPCPRLS RELPEVVEPE DLQDSLDRWY 501 STPFSYPELP DSCQPYGSCF YSLEEEHVGF SLDVDEIEKY QEGEEDQKPP 551 CPRLNEVLME AEEPEVLQDS LDRCYSTTST YFQLHASFQQ YRSAFYSFEE 601 QDVSLALDVD NRFFTLTVIR HHLAFQMGVI FPH BLASTP hits No BLASTP h ts available Alert BLASTP hits for DKFZphtes3_7dl7, frame 2 PIR:T00069 hypothetical protein KIAA0454 - human (fragment), N = 1, Score = 199, P = le-11 PIR:A45592 liver stage antigen LSA-1 - Plasmodium falciparum, N = 1, Score = 158, P = 2 . le-01 >PIR:T00069 hypothetical protein KIAA0454 - human (fragment) Length = 1,882 HSPs: Score = 199 (29.9 bits), Expect = l.Oe-11, P = l.Oe-11 Identities = 74/261 (28%), Positives = 122/261 (46%) Query: 117 EDCKDLIKSMLRDERLLT EEKLAEELGQAEELRQYKVLVHSQERELTQLREKLQEG 172 +D + LI+ + + E L EEKLAEEL A +Y L+ Q REL+ LR+K++EG Sbjct: 964 KDLESLIQRVSQLEAQLPKNGLEEKLAEELRSASWPGKYDSLIQDQARELSYLRQKIREG 1023 Query. 173 RDASRSLNQH LQALLTPDEPDNSQGRDLREQLAEGCRLAQHLVQKLSPENDDD 225 R + +H + LL ++ D G+ REQLA+G +L + L KLS ++ Sb j ct: 1024 RGICYLITRHAKDTVKSFEDLLRSNDIDYYLGQSFREQLAQGSQLTERLTSKLSTKDHKS 1083 Query: 226 EDEDVKVEEAEKVQELYAPREVQKAEEK-EVPEDSLEECAITCSNSHHPCESNQPYGNTR 284 E + +E L RE+Q+ E+ EV + L+ ++T S+SH +S++ +T Sbjct: 1084 EKDQAGLEPLA LRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSSTS 1139 Query: 285 ITFEEDQV—DSTLIDSSΞHDEWLDAVCIIPENESDHEQEEEKGPVSPRNLQESEEEEAP 342 +E + D ++ +H E A P + +S + S + A Sbjct: 1140 FLSDELEACSDMDIVSEYTHYEEKKAS PSHΞDSIHHSSHSAVLSSKPSSTSASQGAK 1196 Query: 343 QESWDEGDWTLSIPPDMSASYQSDRSTFH 371 ES + +L P + S FH Sbjct: 1197 AES-NSNPISLPTPQNTPKEANQAHSGFH 1224 Score = 89 (13.4 bits), Expect = 1. le-01, P = l.Oe-01 Identities = 35/89 (39%), Positives = 44/89 (49%) Query: 464 KDQEEEEDQG PPCPRLSRELPEVVEP-EDLQDSLDRWYSTPFSYPELPDSCQ-PYGS 518 KD + E+DQ P RLSREL E + E LQ LD TP S L DS + P + Sbjct: 1079 KDHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRSPSST 1138 Query: 519 CFYSLEEEHVGFSLDVDEIEKYQEGEEDQKPP 550 F S E E D+D + +Y EE + P Sbjct: 1139 SFLSDELEACS DMDIVΞEYTHYEEKKASP 1167 Score = 73 (11.0 bits), Expect = 4.8e+00, P = 9.9e-01 Identities = 31/88 (35%), Positives = 40/88 (45%) Query: 390 DQVKKEDQEATSP RLSRELLD-EKEPEVLQDSLDRFYSTPFEYLELPDLCQ-PYRSD 444 D ++DQ P RLSREL + EK EVLQ LD TP L D + P + Sbjct: 1080 DHKSEKDQAGLEPLALRLSRELQEKEKVIEVLQAKLDARSLTPSSSHALSDSHRΞPSSTS 1139 Query: 445 FYSLQEQHLGLALDLDRMKKDQEEEEDQGPP 475 F S L D+D + + EE + P Sbjct: 1140 FLS DELEACSDMDIVSEYTHYEEKKASP 1167 Score = 68 (10.2 bits), Expect = 1. le-01, P = 1.0e-01 Identities = 36/156 (23%), Positives = 68/156 (43%) Query: 31 SHGVGRHQELRDPTV PGPTSSATNVSMVVΞAGPWS GEKAEMNILEINKK 79 S G +HQE + TV P P S + V A G ++ ++ + Sbjct: 684 SPGKHQHQEEGNVTVRPFPRPQSLDLGATFTVDAHQLDNQSQPRDPGPQSAFSLPGSTQH 743 Query: 80 SRPQLAENKQQFRNLKQKCLVTQVAYFL-ANRQNNYDYE-DCKDLIKSMLRDERLLTEEK 137 R QL++ KQ++++L++K L+++ F AN Y + L+K + ++ ++ Sbjct: 744 LRSQLSQCKQRYQDLQEKLLLSEATVFAQANELEKYRVMLTGESLVKQDSKQIQVDLQDL 803 Query: 138 LAEELGQAEELRQYKVLVHSQERELTQLREK-LQEG 172 E G++E + + + E L+E L EG Sbjct: 804 GYETCGRSENEAEREETTSPECEEHNSLKEMVLMEG 839 Score = 65 (9.8 bits), Expect = 2.2e-01, P = 2 .0e-01 Identities = 23/96 (23%), Positives = 52/96 (54%) Query: 123 IKSMLRDERLLTEEKLAEELGQAEE LRQYKVLVHSQERELTQLREKLQEGRDASRS 178 ++ + D+ + E + E+ EE LRQ ++ V ++ +L +LR+ L ++ + Sbjct: 5 LRQRIHDKAVALERAIDEKFSALEEKEKELRQLRLAVRERDHDLERLRDVLS ΞNEA 60 Query: 179 LNQHLQALLTPDEPDNSQGRDLREQLAEGCRLAQHLVQKL 218 Q +++LL ++G ++ EQL+ C+ Q L +++ Sbjct: 61 TMQSMESLL RAKGLEV-EQLSTTCQNLQWLKEEM 93 Score = 61 (9.2 bits), Expect = 5.5e-01, P = 4.2e-01 Identities = 27/95 (28%), Positives = 47/95 (49%) Query: 134 TEEK-LAEELGQAEELRQY KVLVHSQERELTQLREKLQEGRDASRSLNQHLQALLT 188 +E K L +LG+ EE R Y +LV +++ L+ +LQ ++L +++L Sbjct: 855 SERKPLENQLGKQEEFRVYGKΞENILV—LRKDIKDLKAQLQNANKVIQNLKSRVRSLSV 912 Query: 189 PDEPDNSQGRDLREQLAEGCRLAQHLVQKLSPENDDDEDE 228 + +S R R+ A G ++ SP + DEDE Sbjct: 913 TSDYSSSLERP-RKLRAVGT LEGSSPHSVPDEDE 945 Score = 57 (8.6 bits), Expect = 1.4e+00, P = 7.5e-01 Identities = 26/92 (28%), Positives = 47/92 (51%) Query: 127 LRDERLLTEEKLAEELGQAEEL RQYKVLVHSQERELTQLREKLQEGRDASRSLNQHL 183 L E LL EK+A Q +E+ R+ ++L+ + L R +L E A R L L Sbjct: 358 LTQEVLLLREKVASVESQGQEISGNRRQQLLLMLEG—LVDERSRLNEALQAERQLYSSL 415 Query: 184 QALLTPDEPDNSQ-GRDLREQLAEGCRLAQHLVQKL 218 P++S+ R L+ +L EG ++ + ++++ Sb j ct: 416 VKFHA—HPESSERDRTLQVEL-EGAQVLRSRLEEV 448 Score = 54 (8.1 bits), Expect = 2.7e+00, P = 9.3e-01 Identities = 61/264 (23%), Positives = 121/264 (45%) Query: 3 LTPTVQGFQWTLRGPDVETSPFGAPRAASHGVGRHQE—LRDPTVPGPTSSATNVSMVVS 60 L+ T Q QW L+ ++ET F + + + + L D SAT ++ Sb j ct: 79 LSTTCQNLQW-LK-EEMETK-FSRWQKEQESIIQQLQTSLHDRNKEVEDLSAT LLCK 132 Query: 61 AGPWSGEKAEMNILEINKKSR PQLAENKQQFRNLKQKCLVTQVAYFLANRQNNYDYE 117 GP E AE + +K R L++ +Q L+ + + + ++ R+ Sbjct: 133 LGPGQSEIAEELCQRLQRKERMLQDLLSDRNKQV—LEHEMEIQGLLQSVSTREQE-SQA 189 Query: 118 DCKDLIKSMLRDERLLTEEKLAEELGQAEELRQYKVLVHSQERELT QLREKLQEG-- 172 + L+++++ ER + L + LG + L + + +Q+ E+T +L ++ +G Sbjct: 190 AAEKLVQALM--ERNSELQALRQYLGGRDSLMS-QAPISNQQAEVTPTGRLGKQTDQGSM 246 Query: 173 RDASRSLNQHLQALLTPDEPDNSQGRDLREQLAEGCRLAQHLVQKLSPENDDDEDEDVKV 232 + SR + L A P ++ G DL + +A G L ++LS N +E E + Sbjct: 247 QIPSRDDSTSLTAKEDVSIPRSTLG-DL-DTVA-G LEKELS—NAKEELELMAK 295 Query: 233 EEAEKVQELYAPREVQKAEEKEVPEDSLEECAIT 266 +E E EL A + + +E+E+ + + ++T Sbjct: 296 KERESQMELSALQSMMAVQEEELQVQAADMESLT 329 Score = 49 (7.4 bits). Expect = 6.3e+00, P = l.Oe+00 Identities = 21/87 (24%), Positives = 39/87 (44%) Query: 192 PDNSQGRDLREQLAEGCRLAQHLVQKLSPENDDDEDEDVKVEEAEKVQELYAPREVQKAE 251 P ++Q LR QL++ + Q L +KL + + E EK + + + K + Sbjct: 738 PGSTQ—HLRSQLSQCKQRYQDLQEKLLLS EATVFAQANELEKYRVMLTGESLVKQD 792 Query: 252 EKEVPEDSLEECAI-TCSNSHHPCESNQ 278 K++ D L++ TC S + E + Sbjct: 793 SKQIQVD-LQDLGYETCGRSENEAEREE 819 Score = 46 (6.9 bits), Expect = 6.3e+00, P = 1.0e+00 Identities = 19/77 (24%), Positives = 39/77 (50%) Query: 112 NNYDYEDCKDLIKSMLRDERLLTEEKLAEELGQAEELRQYKVLVHSQERELTQLREKLQ- 170 + ++ E+ K+ K + E ++T+E L+E QAE R+ + + + + L+E+L Sbjct: 597 DGWEIEEDKE—KGEVMVETVVTKEGLSESSLQAE-FRKLQGKLKNAHNIINLLKEQLVL 653 Query: 171 EGRDASRSLNQHLQALLT 188 ++ + L L LT Sbjct: 654 SSKEGNSKLTPELLVHLT 671 Pedant information for DKFZphtes3_7dl7, frame 2 Report for DKFZphtes3_7dl7.2 [LENGTH] 633 [MW] 72951.15 [pi] 4.40 [HOMOL] PIR:T00069 hypothetical protein KIAA0454 - human (fragment) 2e-ll [BLOCKS] BL00201E [PROSITE] MYRISTYL 2 [PROSITE] CK2_PHOSPHO_SITE 14 [PROSITE] PKC_PHOSPHO_SITE 4 [PROSITE] ASN_GLYCOSYLATION 2 [PFAM] TNFR/NGFR cysteine-rich region [KW] All_Alpha [KW] LOW_COMPLEXITY 4.90 % [KW] COILED_COIL 6.95 % SEQ MPLTPTVQGFQWTLRGPDVETSPFGAPRAASHGVGRHQELRDPTVPGPTSSATNVSMVVS SEG PRD ccccceeeeeeeecccccccccccccccccccccccccccccccccccccceeeeeeeee COILS SEQ AGPWSGEKAEMNILEINKKSRPQLAENKQQFRNLKQKCLVTQVAYFLANRQNNYDYEDCK SEG PRD ccccccchhhhhhhheeecccchhhhhhhhhhhcccccchhhhhhhhhhcccccccccch COILS SEQ DLIKSMLRDERLLTEEKLAEELGQAEELRQYKVLVHSQERELTQLREKLQEGRDASRSLN SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccchhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC SEQ QHLQALLTPDEPDNSQGRDLREQLAEGCRLAQHLVQKLSPENDDDEDEDVKVEEAEKVQE SEG xxxxxxxxxxxxxxxx .. PRD hhhhhhhhccccccccchhhhhhhhhhhhhhhhhhhhhcccccccccchhhhhhhhhhhh COILS CCCCCCC SEQ LYAPREVQKAEEKEVPEDSLEECAITCSNSHHPCESNQPYGNTRITFEEDQVDSTLIDSS SEG PRD hhhcchhhhhhhhhhcchhhhhhhccccccccccccccccccceeeeecccccccccccc COILS SEQ SHDEWLDAVCIIPENEΞDHEQEEEKGPVSPRNLQESEEEEAPQESWDEGDWTLSIPPDMS SEG xxxxxxxxxxxxxxx PRD ccchhhhheeeccccccchhhhhhcccccccccchhhhhhhccccccccccccccccccc COILS SEQ ASYQSDRSTFHSVEEQQVGLALDIGRHWCDQVKKEDQEATSPRLSRELLDEKEPEVLQDS SEG PRD ccccccccchhhhhhhhhhhhhhccccccchhhhhccccccchhhhhhhhhhhheeeecc COILS SEQ LDRFYSTPFEYLELPDLCQPYRSDFYSLQEQHLGLALDLDRMKKDQEEEEDQGPPCPRLS SEG PRD hhhhhccceeeeecccccccccccchhhhhhhhhhhhhcchhhhhhhhhhcccccccccc COILS SEQ RELPEVVEPEDLQDSLDRWYSTPFSYPELPDΞCQPYGSCFYSLEEEHVGFSLDVDEIEKY SEG PRD ccceeeeeccchhhhhhhhhccccccccccccccccccceeeeccceeeccccchhhhhh COILS SEQ QEGEEDQKPPCPRLNEVLMEAEEPEVLQDSLDRCYSTTSTYFQLHASFQQYRSAFYΞFEE SEG PRD hcccccccccccchhhhhhhhhchhhhhccccceeecceeeehhhhhhhhhhhhhhhhhc COILS SEQ QDVSLALDVDNRFFTLTVIRHHLAFQMGVIFPH SEG PRD cchhhhhhcccchhhhhhhhhhhhhhhhhcccc COILS Prosite for DKFZphtes3_7dl7.2 PS00001 54->58 ASN_GLYCOSYLATION PDOC00001 PS00001 315->319 ASN_GLYCOSYLATION PDOC00001 PS00005 13->16 PKC_PHOSPHO_SITE PDOC00005 PS00005 329->332 PKC_PHOSPHO_SITE PDOC00005 PS00005 365->368 PKC_PHOSPHO_SITE PDOC00005 PS00005 401->4O4 PKC_PHOSPHO_SITE PDOC00005 PS00006 188->192 CK2_PHOSPHO_SITE PDOC00006 PS00006 259->263 CK2_PHOSPHO_SITE PDOC00006 PS00006 286->290 CK2_PHOSPHO_SITE PDOC00006 PS00006 295->299 CK2_PHOSPHO_SITE PDOC00006 PS00006 300->304 CK2_PHOSPHO_SITE PDOC00006 PS00006 317->321 CK2_PHOSPHO_SITE PDOC00006 PS00006 336->340 CK2_PHOSPHO_SITE PDOC00006 PS00006 345->349 CK2_PHOSPHO_SITE PDOC00006 PS00006 372->376 CK2_PHOSPHO_SITE PDOC00006 PS00006 427->431 CK2_PHOSPHO_SITE PDOC00006 PS00006 447->451 CK2_PHOΞPHO_SITE PDOC00006 PS00006 505->509 CK2_PHOSPHO_SITE PDOC00006 PS00006 522->526 CK2_PHOSPHO_SITE PDOC00006 PS00006 597->601 CK2_PHOSPHO_SITE PDOC00006 PS00008 25->31 MYRISTYL PDOC00008 PS00008 207->213 MYRISTYL PDOC00008 Pfam for DKFZphtes3_7dl7.2 HMM_NAME TNFR/NGFR cysteine-rich region HMM *CpeGtYtDWNHvpqClpCtrCePEMGQYMvqPCTwTQNTVC* C+ ++ + N+ ++ + ++ + +++ +++ ++VC Query 274 CESNQPYG-NT-RITFEEDQVDS— LIDSSSHDEWLDAVC 310 DKFZphtes3_7j3 group: cell cycle DKFZphtes3_7j3.2 encodes a novel 628 ammo acid putative protein kinase, which is related to the C-TAKl Cdc25C associated protein kinase. Cdc25C is a protein kinase that controls entry into mitosis by dephosphorylation of Cdc2. Cdc25C function is regulated by phosphorylation, too. Serine 216 phosphorylation of Cdc25C mediates the binding of 14-3-3 protein to Cdc 2 5C. C-TAKl (Cdc twenty-five C associated protein kinase) phosphorylates Cdc25C on serine 216 in vitro. The new protein is closely related to C- Takl and therefore should be involved in cell-cycle regulation, too. The new protein can find application in modulating/blocking the cell cycle. strong similarity to serine/threonme-specific protein kinases complete cDNA, complete eds, potential start at Bp 128, few EST hits Sequenced by BMFZ Locus : unknown Insert length: 3443 bp Poly A stretch at pos. 3399, polyadenylation signal at pos. 3376 1 GTGCTTTACT GCGCGCTCTG GTACTGCTGT GGCTCCCCGT CCTGGTGCGG 51 GACCTGTGCC CCGCGCTTCA GCCCTCCCCG CACAGCCTAC TGATTCCCCT 101 GCCGCCCTTG CTCACCTCCT GCTCGCCATG GAGTCGCTGG TTTTCGCGCG 151 GCGCTCCGGC CCCACTCCCT CGGCCGCAGA GCTAGCCCGG CCGCTGGCGG 201 AAGGGCTGAT CAAGTCGCCC AAGCCCCTAA TGAAGAAGCA GGCGGTGAAG 251 CGGCACCACC ACAAGCACAA CCTGCGGCAC CGCTACGAGT TCCTGGAGAC 301 CCTGGGCAAA GGCACCTACG GGAAGGTGAA GAAGGCGCGG GAGAGCTCGG 351 GGCGCCTGGT GGCCATCAAG TCAATCCGGA AGGACAAAAT CAAAGATGAG 401 CAAGATCTGA TGCACATACG GAGGGAGATT GAGATCATGT CATCACTCAA 451 CCACCCTCAC ATCATTGCCA TCCATGAAGT GTTTGAGAAC AGCAGCAAGA 501 TCGTGATCGT CATGGAGTAT GCCAGCCGGG GCGACCTTTA TGACTACATC 551 AGCGAGCGGC AGCAGCTCAG TGAGCGCGAA GCTAGGCATT TCTTCCGGCA 601 GATCGTCTCT GCCGTGCACT ATTGCCATCA GAACAGAGTT GTCCACCGAG 651 ATCTCAAGCT GGAGAACATC CTCTTGGATG CCAATGGGAA TATCAAGATT 701 GCTGACTTCG GCCTCTCCAA CCTCTACCAT CAAGGCAAGT TCCTGCAGAC 751 ATTCTGTGGG AGCCCCCTCT ATGCCTCGCC AGAGATTGTC AATGGGAAGC 801 CCTACACAGG CCCAGAGGTG GACAGCTGGT CCCTGGGTGT TCTCCTCTAC 851 ATCCTGGTGC ATGGCACCAT GCCCTTTGAT GGGCATGACC ATAAGATCCT 901 AGTGAAACAG ATCAGCAACG GGGCCTACCG GGAGCCACCT AAACCCTCTG 951 ATGCCTGTGG CCTGATCCGG TGGCTGTTGA TGGTGAACCC CACCCGCCGG 1001 GCCACCCTGG AGGATGTGGC CAGTCACTGG TGGGTCAACT GGGGCTACGC 1051 CACCCGAGTG GGAGAGCAGG AGGCTCCGCA TGAGGGTGGG CACCCTGGCA 1101 GTGACTCTGC CCGCGCCTCC ATGGCTGACT GGCTCCGGCG TTCCTCCCGC 1151 CCCCTCCTGG AGAATGGGGC CAAGGTGTGC AGCTTCTTCA AGCAGCATGC 1201 ACCTGGTGGG GGAAGCACCA CCCCTGGCCT GGAGCGCCAG CATTCGCTCA 1251 AGAAGTCCCG CAAGGAGAAT GACATGGCCC AGTCTCTCCA CAGTGACACG 1301 GCTGATGACA CTGCCCATCG CCCTGGCAAG AGCAACCTCA AGCTGCCAAA 1351 GGGCATTCTC AAGAAGAAGG TGTCAGCCTC TGCAGAAGGG GTACAGGAGG 1401 ACCCTCCGGA GCTCAGCCCA ATCCCTGCGA GCCCAGGGCA GGCTGCCCCG 1451 CTGCTCCCCA AGAAGGGCAT TCTCAAGAAG CCCCGACAGC GCGAGTCTGG 1501 CTACTACTCC TCTCCCGAGC CCAGTGAATC TGGGGAGCTC TTGGACGCAG 1551 GCGACGTGTT TGTGAGTGGG GATCCCAAGG AGCAGAAGCC TCCGCAAGCT 1601 TCAGGGCTGC TCCTCCATCG CAAAGGCATC CTCAAACTCA ATGGCAAGTT 1651 CTCCCAGACA GCCTTGGAGC TCGCGGCCCC CACCACCTTC GGCTCCCTGG 1701 ATGAACTCGC CCCACCTCGC CCCCTGGCCC GGGCCAGCCG ACCCTCAGGG 1751 GCTGTGAGCG AGGACAGCAT CCTGTCCTCT GAGTCCTTTG ACCAGCTGGA 1801 CTTGCCTGAA CGGCTCCCAG AGCCCCCACT GCGGGGCTGT GTGTCTGTGG 1851 ACAACCTCAC GGGGCTTGAG GAGCCCCCCT CAGAGGGCCC TGGAAGCTGC 1901 CTGAGGCGCT GGCGGCAGGA TCCTTTGGGG GACAGCTGCT TTTCCCTGAC 1951 AGACTGCCAG GAGGTGACAG CGACCTACCG ACAGGCACTG AGGGTCTGCT 2001 CAAAGCTCAC CTGAGTGGAG TAGGCATTGC CCCAGCCCGG TCAGGCTCTC 2051 AGATGCAGCT GGTTGCACCC CGAGGGGAGA TGCCTTCTCC CCCACCTCCC 2101 AGGACCTGCA TCCCAGCTCA GAAGGCTGAG AGGGTTTGCA GTGGAGCCCT 2151 GAGCAGGGCT GGATATGGGA AGTAGGCAAA TGAAATGCGC CAAGGGTTCA 2201 GTGTCTGTCT TCAGCCCTGC TGAACGAAGA GGATACTAAA GAGAGGGGAA 2251 CGGGAATGCC CGCGACAGAG TCCACATTGC CTGTTTCTTG TGTACATGGG 2301 GGGGCCACAG AGACCTGGAA AGAGAACTCT CCCAGGGCCC ATCTCCTGCA 2351 TCCCATGAAT ACTCTGTACA CATGGTGCCT TCTAAGGACA GCTCCTTCCC 2401 TACTCATTCC CTGCCCAAGT GGGGCCAGAC CTCTTTACAC ACACATTCCC 2451 GTTCCTACCA ACCACCAGAA CTGGATGGTG GCACCCCTAA TGTGCATGAG 2501 GCATCCTGGG AATGGTCTGG AGTAACGCTT CGTTATTTTT ATTTTTATTT 2551 TTATTTATTT ATTTATTTTT TTGAGACGGA GTTTCGCTCT TGGTGCCCAG 2601 GCTAGAGTGC AATGGCGCGA TCTCAGCTCA CCTCAACCTC CGCCTCCCGG 2651 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC TAGTAGCTGG GATTACAGGC 2701 GCCCGCCACC ATGCCCGGCT AATTTTGTAT TTTTAGTAGA GACAGGGTTT 2751 CTCCATGTTG GTCAGGCTGG TCTCAAACTC CCGACCTCAG GTGATCCACC 2801 CACCTCGGCC TCCCAAAGTG CTGGGATTAC AGGCGTGAGC CACCGCGCCC 2851 CACCTAACCC TTCCTTATTT AGCCTAGGAG TAAGAGAACA CAATCTCTGT 2901 TTCTTCAATG GTTCTCTTCC CTTTTCCATC CTCCAAACCT GGCCTGAGCC 2951 TCCTGAAGTT GCTGCTGTGA ATCTGAAAGA CTTGAAAAGC CTCCGCCTGC 3001 TGTGTGGACT TCATCTCAAG GGGCCCAGCC TCCTCTGGAC TCCACCTTGG 3051 ACCTCAGTGA CTCAGAACTT CTGCCTCTAA GCTGCTCTAA AGTCCAGACT 3101 ATGGATGTGT TCTCTAGGCC TTCAGGACTC TAGAATGTCC ATATTTATTT 3151 TTATGTTCTT GGCTTTGTGT TTTAGGAAAA GTGAATCTTG CTGTTTTCAA 3201 TAATGTGAAT GCTATGTTCT GGGAAAATCC ACTATGACAT CTAAGTTTTG 3251 TGTACAGAGA GATATTTTTG CAACTATTTC CACCTCCTCC CACAACCCCC 3301 CACACTCCAC TCCACACTCT TGAGTCTCTT TACCTAATGG TCTCTACCTA 3351 ATGGACCTCC GTGGCCAAAA AGTACCATTA AAACCAGAAA GGTGATTGGA 3401 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAA BLAST Results No BLAST result Medline entries 98202387: C-TAKl protein kinase phosphorylates human Cdc25C on serine 216 and promotes 14-3-3 protein binding. Peptide information for frame 2 ORF from 128 bp to 2011 bp; peptide length: 628 Category: strong similarity to known protein 1 MESLVFARRS GPTPSAAELA RPLAEGLIKS PKPLMKKQAV KRHHHKHNLR 51 HRYEFLETLG KGTYGKVKKA RESSGRLVAI KSIRKDKIKD EQDLMHIRRE 101 IEIMSSLNHP HIIAIHEVFE NSSKIVIVME YASRGDLYDY ISERQQLSER 151 EARHFFRQIV SAVHYCHQNR VVHRDLKLEN ILLDANGNIK IADFGLSNLY 201 HQGKFLQTFC GSPLYASPEI VNGKPYTGPE VDSWSLGVLL YILVHGTMPF 251 DGHDHKILVK QISNGAYREP PKPSDACGLI RWLLMVNPTR RATLEDVASH 301 WWVNWGYATR VGEQEAPHEG GHPGSDSARA SMADWLRRSS RPLLENGAKV 351 CSFFKQHAPG GGSTTPGLER QHSLKKSRKE NDMAQSLHSD TADDTAHRPG 401 KSNLKLPKGI LKKKVSASAE GVQEDPPELS PIPASPGQAA PLLPKKGILK 451 KPRQRESGYY SSPEPSESGE LLDAGDVFVS GDPKEQKPPQ ASGLLLHRKG 501 ILKLNGKFSQ TALELAAPTT FGSLDELAPP RPLARASRPS GAVSEDSILS 551 SEΞFDQLDLP ERLPEPPLRG CVSVDNLTGL EEPPSEGPGS CLRRWRQDPL 601 GDSCFSLTDC QEVTATYRQA LRVCSKLT BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_7j3, frame 2 No Alert BLASTP hits found Pedant information for DKFZphtes3_7j3, frame 2 Report for DKFZphtes3_7j3.2 [LENGTH] 628 [MW] 69612.39 [pi] 9.01 [HOMOL] TREMBL:AB011109_1 gene: "KIAA0537"; product: "KIAA0537 protein"; Homo sapiens mRNA for KIAA0537 protein, complete eds. le-152 [FUNCAT] 01.05.04 regulation of carbohydrate utilization [S. cerevisiae, YDR477w] 5e-66 [FUNCAT] 11.01 stress response [S. cerevisiae, YDR477w] 5e-66 FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YDR477w] 5e-66 FUNCAT] 98 classification not yet clear-cut [S. cerevisiae, YLR096w] 6e-54 FUNCAT] 30.02 organization of plasma membrane [S. cerevisiae, YLR096w] 6e-54 FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YDR507c] e-52 FUNCAT] 03.25 cytokinesis [S. cerevisiae, YDR507c] 8e-52 FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YKLlOlw] 9e-51 FUNCAT] 30.10 nuclear organization [S. cerevisiae, YKLlOlw] 9e-51 FUNCAT] 99 unclassified proteins [S. cerevisiae, YPL141c] le-45 FUNCAT] 10.99 other signal-transduction activities [S. cerevisiae, YPL153c] 6e-44 FUNCAT] 03.22.01 cell cycle check point proteins [S. cerevisiae, YPL153c] 6e-44 FUNCAT] 11.04 dna repair (direct repair, base excision repair and nucleotide excision repair) [S. cerevisiae, YPL153c] 6e-44 FUNCAT] 03.19 recombination and dna repair [S. cerevisiae, YPL153c] 6e-44 FUNCAT] 03.16 dna synthesis and replication [Ξ. cerevisiae, YMROOlc] 2e-42 FUNCAT] 10.02.11 key kinases [S. cerevisiae, YBL105c] 3e-34 FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YKL139w CTK1 - carboxy- ermmal domain] 2e-28 FUNCAT] 03 01 cell growth [S. cerevisiae, YFR014c] 4e-28 FUNCAT] 03 10 sporulation and germination [S. cerevisiae, YGL180w] 2e-26 FUNCAT] 06 13.04 lysosomal and vacuolar degradation [S. cerevisiae, YGL180w] 2e-26 FUNCAT] 08 13 vacuolar transport [S. cerevisiae, YGL180w] 2e-26 FUNCAT] 04 99 other transcription activities [S. cerevisiae, YER129w] 4e-26 FUNCAT] 02 19 metabolism of energy reserves (glycogen, trehalose) [S. cerevisiae, YPL031C] 5e-24 FUNCAT] 01 04.04 regulation of phosphate utilization [S. cerevisiae, YPL031c] e-24 FUNCAT ] 03.07 pheromone response mating-type determination, sex-specific proteins [S. cerevisiae, YHL007c] 6e-24 FUNCAT] 10.05.11 key kinases [S. cerevisiae, YHL007c] 6e-24 FUNCAT] 09.01 biogenesis of cell wall [S. cerevisiae, YNR031C] le-22 FUNCAT] 10.03.11 key kinases [S. cerevisiae, YNR031c] le-22 FUNCAT] 03.13 meiosis [S. cerevisiae, YDR523c] 8e-22 FUNCAT] 04.05.01.01 general transcription activities [S. cerevisiae, YDL108w] e-21 FUNCAT] 06.07 protein modification (glyeolsylation, acylation, myristylation, palmitylation farnesylation and processing) [S. cerevisiae, YFL033c] 6e-21 FUNCAT] 10.05.09 regulation of g-protein activity [S. cerevisiae, YBL016w] 7e-19 FUNCAT] 10.04.11 key kinases [Ξ. cerevisiae, YDL159w] 3e-18 FUNCAT] 01.02.04 regulation of nitrogen and sulphur utilization [S. cerevisiae, YNL183C] le-17 FUNCAT] 08.99 other mtracellular-transport activities [S. cerevisiae, YNL183c] e-17 FUNCAT] 05, 07 translational control [S. cerevisiae, YDR283c] 2e-17 FUNCAT] 09, 04 biogenesis of cytoskeleton [S. cerevisiae, YNL020c] 4e-16 FUNCAT] 04.03.99 other trna-transcription activities [S. cerevisiae, YOR061w] e-15 FUNCAT] 10.04.99 other nutritional-response activities [S. cerevisiae, YJR059w] 5e-15 FUNCAT] c energy conversion [M. gemtalium, MG109] 3e-12 FUNCAT] 30.09 organization of intracellular transport vesicles [S. cerevisiae, YBR097W] 2e-08 FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YBR097w] e-08 FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YBR097w] e-08 FUNCAT] 30. organization of golgi [S. cerevisiae, YBR097w] 2e-08 FUNCAT] 30. organization of endoplasmatic reticulum [S. cerevisiae, YHR079c] e-05 FUNCAT] 01.06.10 regulation of lipid, fatty-acid and sterol biosynthesis [S. cerevisiae, YHR079C] 8e-05 BLOCKS] BL00479C Phorbol esters / diacylglycerol binding domain proteins BLOCKS] BL00239B Receptor tyrosine kinase class II proteins BLOCKS] BL00107A Protein kinases ATP-binding region proteins SCOP] dlgol 5.1.1.1.9 MAP kinase Erk2 [rat Rattus norvegicus le-77 SCOP] dlwfc 5.1.1.1.8 MAP kinase p38 [human (Homo sapiens) 4e-68 SCOP] dlkoa_2 5.1.1.1.7 (1-350) Twitchm, kinase domain [Caenorhabditi 2e-85 SCOP] dlkoba_ 5.1.1.1.6 Twitchm, kinase domain [California sea har le-80 SCOP] dlphk 5.1.1.1.5 gamma-subunit of glycogen phosphorylase kinas 2e-76 SCOP] dlirk 5.1.1.2.4 insulin receptor [Human (Homo sapiens) le-69 SCOP] dlapme_ 5.1.1.1.4 cAMP-dependent PK, catalytic subunit [mouse (Mu le-84 SCOP] dlfgka_ 5.1.1.2.3 Fibroblast growth factor receptor 1 [human (Hom le-68 SCOP] dlydre_ 5.1.1.1.3 cAMP-dependent PK, catalytic subunit [bovine (Bo 9e-85 SCOP] dlfmk_3 5.1.1.2.2 (168-437) c-src tyrosine kinase [human (Hom le-69 SCOP] dlcdka_ 5.1.1.1.2 cAMP-dependent PK, catalytic subunit [pig (Su le-85 SCOP] d2hcka3 5.1.1.2.1 (167-437) Haemopoetic cell kinase Hck [huma 5e-66 SCOP] dlcsn 5.1.1.1.11 Casein kιnase-1, CK1 [Schizosaccharomyces pombe 9e-47 SCOP] dljsua_ 5.1.1.1.1 Cyclin-dependent PK [Human (Homo sapiens) le-75 SCOP] dlckja_ 5.1.1.1.10 Casein kιnase-1, CK1 [rat (Rattus norvegicus) 5e-54 EC] 2.7.1.38 Phosphorylase kinase le-36 EC] 2.7.1.123 Ca2+/calmodulιn-dependent protein kinase 4e-40 [EC] 2.7.1.128 [Acetyl-CoA carboxylase] kinase le-61 [EC] 2.7.1.117 Myosin-light-chain kinase 2e-40 [EC] 2.7.1.109 [Hydroxymethylglutaryl-CoA reductase (NADPH) ] kinase le-61 [EC] 2.7.1.37 Protein kinase 7e-42 [PIRKW] phosphotransferase 6e-66 [PIRKW] nucleus le-64 [PIRKW] calcium 7e-35 [PIRKW] duplication le-38 [PIRKW] tandem repeat 4e-39 [PIRKW] phorbol ester binding le-38 [PIRKW] zinc le-38 [PIRKW] cell cycle control le-42 [PIRKW] serme/threonme-specific protein kinase 8e-68 [PIRKW] oncogene le-40 [PIRKW] phospholipid binding le-38 [PIRKW] autophosphorylation le-64 [PIRKW] brain le-40 [PIRKW] heterotetramer 2e-36 [PIRKW] mitosis 7e-42 [PIRKW] polymer le-35 [PIRKW] magnesium 6e-66 [PIRKW] ATP 8e-68 [PIRKW] polyprotein le-40 [PIRKW] phosphoprotem le-64 [PIRKW] apoptosis 4e-39 [PIRKW] glycoprotein 7e-42 [PIRKW] leucine zipper 3e-35 [PIRKW] skeletal muscle 7e-35 [PIRKW] protein kinase 5e-41 [PIRKW] cAMP binding 3e-38 [PIRKW] testis 9e-36 [PIRKW] purme nucleotide binding 2e-49 [PIRKW] calcium binding 8e-39 [PIRKW] alternative splicing 3e-37 [PIRKW] P-loop 2e-49 [PIRKW] lipoprotein 2e-33 [PIRKW] segmentation le-33 [PIRKW] core protein le-40 [PIRKW] muscle 7e-35 [PIRKW] myristylation 2e-33 [PIRKW] EF hand 8e-39 [PIRKW] cell division 2e-40 [PIRKW] calmodulin binding 4e-40 [SUPFAM] ribosomal protein S6 kinase II 5e-36 [SUPFAM] fibronectin type III repeat homology 3e-33 [SUPFAM] lmmunoglobulin homology 3e-33 [SUPFAM] calcium-dependent protein kinase 8e-39 [SUPFAM] AMP-activated protein kinase 6e-66 [SUPFAM] protein kinase akt 3e-42 [SUPFAM] protein kinase SPK1 le-42 [SUPFAM] unassigned Ser/Thr or Tyr-specific protein kinases 8e-68 [SUPFAM] Ca2+/calmodulιn-dependent protein kinase 3e-37 [SUPFAM] calmodulin repeat homology 8e-39 [SUPFAM] cAMP receptor protein cyclic nucleotide-binding domain homology 6e-33 [SUPFAM] protein kinase C zeta le-36 [SUPFAM] Dictyostelium cAMP-dependent protein kinase catalytic chain le-34 [SUPFAM] death-associated protein kinase 4e-39 [SUPFAM] pleckstrm repeat homology 3e-42 [SUPFAM] ankyrin repeat homology 4e-39 [SUPFAM] protein kinase homology 8e-68 [SUPFAM] Ca2+/calmodulm-dependent protein kinase II 8e-41 [SUPFAM] protein kinase C zmc-bmding repeat homology le-38 [SUPFAM] twitchm 3e-33 [SUPFAM] protein kinase C delta le-38 [SUPFAM] cGMP-dependent protein kinase 6e-33 [SUPFAM] protein kinase cdrl 7e-42 [SUPFAM] protein kinase C C2 region homology 3e-37 [SUPFAM] protein kinase C alpha 3e-37 [SUPFAM] yeast protein kinase C 5e-36 [SUPFAM] kinase-related transforming protein le-41 [SUPFAM] kinase interaction domain homology le-42 [SUPFAM] gag-akt polyprotein le-40 [SUPFAM] Ca2+/calmodulιn-dependent protein kinase I 4e-40 [SUPFAM] protein kinase C mu 4e-33 [PROSITE] PROTEIN_KINASE_ATP 2 [PROSITE] RGD 1 [PROSITE] MYRISTYL 4 [PROSITE] CAMP_PHOSPHO_SITE 3 [PROSITE] CK2_PHOSPHO_SITE 13 [PROSITE] TYR_PHOSPHO_SITE 2 [PROSITE] PKC PHOSPHO_SITE 12 [PROSITE] ASN_GLYCOSYLATION 2 [PROSITE] PROTEIN_KINASE_ST 1 [PFAM] Eukaryotic protein kinase domain [KW] All_Alpha [KW] 3D [KW] LOW COMPLEXITY 10.51 % SEQ MESLVFARRSGPTPSAAELARPLAEGLIKSPKPLMKKQAVKRHHHKHNLRHRYEFLETLG SEG xxxxxxxxxxxx IctpE HHHHHHHHHHHHHHHCCCCCCCC—GGGEEEEEEEE SEQ KGTYGKVKKARESSGRLVAIKSIRKDKIKDEQDLMHIRREIEIMSSLNHPHIIAIHEVFE SEG IctpE CTTTEEEEEEEETTTEEEEEEEEEHHHHHHHCCHHHHHHHHHHHHCCCTTTBCCEEEEEE SEQ NSSKIVIVMEYASRGDLYDYISERQQLΞEREARHFFRQIVSAVHYCHQNRVVHRDLKLEN SEG IctpE ETTEEEEEEECTTTTBHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEECCCCCGGG SEQ ILLDANGNIKIADFGLSNLYHQGKFLQTFCGSPLYAΞPEIVNGKPYTGPEVDSWSLGVLL SEG IctpE EEETTTTCEEECCTTTTEET-TTT-BCCCCCCGGGCCHHHHHCCCBC-HHHHHHHHHHHH SEQ YILVHGTMPFDGHDHKILVKQISNGAYREPPKPSDACGLIRWLLMVNPTRRATLEDVASH SEG IctpE HHHHHCCTTTTTTTHHHHHHHHHHCCCCCTTTCHHHHHHHHHTTTTTGGGTTTHHHHHHC SEQ WWVNWGYATRVGEQEAPHEGGHPGSDSARASMADWLRRSSRPLLENGAKVCSFFKQHAPG SEG IctpE GG SEQ GGSTTPGLERQHSLKKSRKENDMAQSLHSDTADDTAHRPGKSNLKLPKGILKKKVSASAE SEG IctpE SEQ GVQEDPPELSPIPASPGQAAPLLPKKGILKKPRQRESGYYSSPEPSESGELLDAGDVFVS SEG xxxxxxxxxxxx ... xxxxxxxxxxxxxxx IctpE SEQ GDPKEQKPPQASGLLLHRKGILKLNGKFSQTALELAAPTTFGSLDELAPPRPLARASRPS SEG xxxxxxxxxxxxxx IctpE SEQ GAVSEDSILSSESFDQLDLPERLPEPPLRGCVSVDNLTGLEEPPSEGPGSCLRRWRQDPL SEG xxxxxxxxxxxxx IctpE SEQ GDSCFSLTDCQEVTATYRQALRVCSKLT SEG IctpE Prosite for DKFZphtes3_7j3.2 PS00001 121->125 ASN_GLYCOSYLATION PDOC00001 PS00001 576->580 ASN_GLYCOSYLATION PDOC00001 PS00004 290->294 CAMP_PHOSPHO_SITE PDOC00004 PS00004 337->341 CAMP_PHOSPHO_SITE PDOC00004 PS00004 413->417 CAMP_PHOSPHO_SITE PDOC00004 PS00005 30->33 PKC_PHOSPHO_SITE PDOC00005 PS00005 74->77 PKC_PHOSPHO_SITE PDOC00005 PS00005 82->85 PKC_PHOSPHO_SITE PDOC00005 PS00005 122->125 PKC_PHOSPHO_SITE PDOC00005 PS00005 142->145 PKC_PHOSPHO_SITE PDOC00005 PS00005 148->151 PKC_PHOSPHO_SITE PDOC00005 PS00005 289->292 PKC_PHOSPHO_SITE PDOC00005 PS00005 327->330 PKC_PHOSPHO_SITE PDOC00005 PS00005 339->342 PKC_PHOSPHO_SITE PDOC00005 PS00005 373->376 PKC_PHOSPHO_SITE PDOC00005 PS00005 377->380 PKC_PHOSPHO_SITE PDOC00005 PΞ00005 616->619 PKC_PHOSPHO_SITE PDOC00005 PS00006 15->19 CK2_PHOSPHO_SITE PDOC00006 PS00006 133->137 CK2_PHOSPHO_SITE PDOC00006 PS00006 148->152 CK2_PHOSPHO_ΞITE PDOC00006 PS00006 227->231 CK2_PHOSPHO_SITE PDOC00006 PS00006 293->297 CK2_PHOSPHO_SITE PDOC00006 PS00006 331->335 CK2_PHOSPHO_SITE PDOC00006 PS00006 377->381 CK2_PHOSPHO_SITE PDOC00006 PS00006 391->395 CK2 PHOSPHO SITE PDOC00006 PS00006 461->465 CK2_PHOSPHO_SITE PDOC00006 PS00006 511->515 CK2_PHOSPHO_SITE PDOC00006 PS00006 523->527 CK2_PHOSPHO_SITE PDOC00006 PS00006 578->582 CK2_PHOSPHO_SITE PDOC00006 PS00006 606->610 CK2_PHOSPHO_SITE PDOC00006 PS00007 453->460 TYR_PHOSPHO_SITE PDOC00007 PS00007 453->461 TYR_PHOSPHO_SITE PDOC00007 PS00008 320->326 MYRISTYL PDOC00008 PS00008 324->330 MYRISTYL PDOC00008 PS00008 347->353 MYRISTYL PDOC00008 PS00008 360->366 MYRISTYL PDOC00008 PS00016 134->137 RGD PDOC00016 PS00107 59->82 PROTEIN_KINASE_ATP PDOC00100 PS00107 59->86 PROTEIN_KINASE_ATP PDOC00100 PS00108 171->184 PROTEIN KINASE ST PDOC00100 Pfam for DKFZphtes3_7j3.2 HMM_NAME Eukaryotic protein kinase domain HMM *YeιgRιIGeGsFGtVYkCιWrTGeIVAIKIIkkrsms F1REI YE+++++G+G++G+V+K+++ +G++VAIK I+K++++ ++REI Query 53 YEFLETLGKGTYGKVKKARESSGRLVAIKSIRKDKIKDEQDLMHIRREI 101 HMM qlMRrLnHPNIIRFYDwFedddDHIYMIMEYMeGGDLFDYIrrngpMsEw +IM +LNHP+II + ++FE ++ I ++MEY+ GDL+DYI+++ ++SE+ Query 102 EIMSSLNHPHIIAIHEVFE-NSSKIVIVMEYAΞRGDLYDYISERQQLSER 150 HMM elrflMyQILrGMeYLHSMgllHRDLKPENILIDeNgqlKIcDFGLARqM E+R++++QI++++ Y+H ++++HRDLK ENIL+D NG+IKI+DFGL+ ++ Query 151 EARHFFRQIVSAVHYCHQNRVVHRDLKLENILLDANGNIKIADFGLSNLY 200 HMM nnYerMttfCGTPWYMMAPEVIImg.nyYttkVDMWSFGCILWEMMTGep + + ++ TFCG+P Y +PE+ ++G +Y +++VD WS+G++L++++ G+ Query 201 HQGKFLQTFCGSPLYA-SPEI-VNGKPYTGPEVDSWSLGVLLYILVHGTM 248 HMM PFyddnMemlmrliqrfrrpfWpnCSeElyDFMrwCWnyDPekRPTFrQI PF+++ ++ I + +++ +P S+ + ++RW++ ++P++R T +++ Query 249 PFDGHDHKILVKQISNGAYREPPKPSD-ACGLIRWLLMVNPTRRATLEDV 297 HMM LnHPWF* H W+ Query 298 ASHWWV 303 DKFZphtes3_7 8 group: testes derived DKFZphtes3_7j8 encodes a novel 410 amino acid protein nearly identical to human WUGSC :H_DJ1159O04.1. The novel protein contains an additional C-terminal domain, which is not present m WUGSC :H_DJ1159O04.1. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . WUGSC :H_DJ1159O04.1 similarity to YBL104p verifies and extends the genmodel WUGSC : H_DJ1159O04.1 similarity to S. cerevisiae YBL104p Sequenced by BMFZ Locus: /map="7p21-p22" Insert length: 3353 bp Poly A stretch at pos. 3231, no polyadenylation signal found 1 GCAAAATATG TTGTATTTGT GGCATAGTTC ATATTTACAC TATCATAAAA 51 TTATGGCCGA GAAGTTAAAT ATTCTAAATG TGTCAACATA GTTCTCTGTA 101 AAACTGACTT ATTTTCCAAA TATATTTTGA AATAAAACAA TATAAAAATG 151 TTTTCTGTTT TTAGGAATGG TGGAAAGCAG CAGACATAAT TGGAGTGGGT 201 TGGATAAGCA AAGTGATATT CAAAATTTAA ATGAAGAGAG AATCTTAGCT 251 TTACAGCTTT GTGGGTGGAT AAAGAAAGGA ACGGATGTAG ACGTGGGGCC 301 ATTTTTGAAC TCCCTTGTAC AAGAAGGGGA ATGGGAAAGA GCTGCTGCTG 351 TGGCATTGTT CAACTTGGAT ATTCGCCGAG CAATCCAAAT CCTGAATGAA 401 GGGGCATCTT CTGAAAAAGG AGATCTGAAT CTCAATGTGG TAGCAATGGC 451 TTTATCGGGT TATACGGATG AGAAGAACTC CCTTTGGAGA GAAATGTGTA 501 GCACACTGCG ATTACAGCTA AATAACCCGT ATTTGTGTGT CATGTTTGCA 551 TTTCTGACAA GTGAAACAGG ATCTTACGAT GGAGTTTTGT ATGAAAACAA 601 AGTTGCAGTA CGTGACAGAG TGGCATTTGC TTGTAAATTC CTTAGTGATA 651 CTCAGTTAAA TAGATACATC GAAAAGTTGA CCAATGAAAT GAAAGAGGCT 701 GGAAATTTGG AAGGAATTTT GCTTACAGGC CTTACTAAAG ATGGAGTGGA 751 CTTAATGGAG AGTTATGTTG ATAGAACTGG AGATGTTCAA ACAGCAAGTT 801 ACTGTATGTT ACAGGGTTCA CCTTTAGATG TTCTTAAAGA TGAAAGGGTT 851 CAGTACTGGA TTGAGAATTA TAGAAATTTA TTAGATGCCT GGAGGTTTTG 901 GCATAAACGA GCTGAATTTG ATATTCACAG GAGTAAGTTG GATCCCAGTT 951 CCAAGCCTTT AGCACAAGTT TTTGTGAGTT GCAATTTCTG TGGCAAGTCA 1001 ATCTCCTACA GCTGTTCAGC TGTGCCTCAT CAGGGCAGAG GTTTTAGTCA 1051 GTATGGTGTG AGTGGCTCAC CAACGAAATC TAAAGTCACA AGTTGTCCTG 1101 GCTGTCGAAA ACCACTTCCT CGATGTGCGC TTTGTCTCAT TAATATGGGA 1151 ACACCAGTTT CTAGCTGTCC TGGAGGAACC AAATCAGATG AAAAAGTGGA 1201 CTTGAGCAAG GACAAAAAAT TAGCCCAATT TAACAACTGG TTTACATGGT 1251 GTCATAATTG CAGGCACGGT GGACATGCTG GACATATGCT TAGTTGGTTC 1301 AGGGACCATG CAGAGTGCCC TGTGTCTGCA TGCACGTGTA AATGTATGCA 1351 GTTGGATACA ACGGGGAATC TGGTACCTGC AGAGACTGTC CAGCCATAAA 1401 ATGTTACCAC CTTAAGAGAA CCCTTCAAGT GTGGAGCTTT CTAGTAGGTG 1451 TCCTTCATAG CTCAGAAACA TACCTCAGAA CAAGCCATTC ATGACTTACC 1501 TGTAATGGGA AAATAAATCA TTCTATCAGA TCAGCAGTTT TGATGTTTGA 1551 GTGATTTTGA TATGCTTCAC AGAGACAAAT GCTGCCAAAA TAAACATCGA 1601 AGTATAGACA TGAGTTCTGT TCAGCAGGTT GAAAAGTCTG ATTTAGAAAA 1651 ACTTTCTAAG TTTTGGTTGA AATTATGAAC ACTCTAGAAG CAGAATTTCT 1701 GGAAGAGCCA AGAACAGACT TTGAGCCTAT ATCTTCAAAG CTGAAACTGG 1751 ATATCTTTCA ATAAAATATG TGCACTTTTA AAATAAAATG ACTAATTCTG 1801 TGATTCAGAC AATAGTTTTA AGTTCAGCTG TGCTTAGATT TCTTTCAGAT 1851 TAATTTAAAA TTATAGATTT TTACTTTTAG AATTGCAGAG CCCCTATCCC 1901 ACACTGGAGA ATATTTTTTA TTACTGTCTG TTATATATGT GTCTATGTGT 1951 GTGTGTATAT TTATGTGTGT ATGTATAAAT ATGTACTTTT TAAAGGAGCC 2001 TTTTCCCTCC TTTGATTTTA AGATAAGCAA TCTTTTGGCA TAACATTATC 2051 GTCTTCCTAG AAAAGCCAAG ATGAAGAATC TATCTTACAA CTTTTTCTCT 2101 TCAGTAGAGA AAAACATGTA CCATTTCAGG TGAACATACA AAATTTTCAC 2151 TTTCTACCTT TTGCCTTCCA ATGTCCTGAT TTGTCTTCAA AGGTTTTTCT 2201 CCATATTAAT TTGTCATCTT ATCCTCATCA CCTGAGAACA TTTTACTGCA 2251 TACAAAGTCT ATGCAAGATT ATATGTAACT AGCCATTTAG TATAATCTAT 2301 GTCAGTGTTT CTGTGCTGTC AAATTCCGTC CTGATTTGGA ATACCATACC 2351 TTGTTCTTTC CAAGGTAGAC TAGGAAGTGT TGGGGAAATA GGGTCACTTC 2401 AGAGACCATT TTAGATGTAA GTTTTTAAAT GTAAGTGTTA CTGGGGCTAA 2451 GTCAGGGACT TTATTTAAAA CATTTTTTTT TTCTCATTTC ATAGCTAGAT 2501 AGTTGTAAGA GAAATACAAA GAATTTACAA GATGCTTCTC TGTCATCTGC 2551 CGTATGCAGA GGGACTGAAC TAGGAATTTT GTAGTTGAAG CTGTGTTCAT 2601 AAAGAGTAAA TCTTATTTTA TAGATTTTGG AGAAATAAAA CAAGAATTTT 2651 AAGAGCTTTC GTATTAGCAG TTTTGCCTTA TAAAAACTAA GATTTGTCAG 2701 ATTAGTTTGA GGTGTAACCT AAATATTAAA AGTAGATTAA ATTTATTTTT 2751 TACCTTGAGT GTCTGATACA TAAAACCCTT TTCTAGGAAA ACATTGGAAG 2801 TAGTACATAT TTACTCTAAA TGTCTCACCT GCATGACAGT CTTTTCAAAT 2851 GAAAGACATG GTAATTGCAA TTTTTTTTTA AAGATTGCTA TTAAGGGTAC 2901 TTTTTCCAGC CTTCATTTGA GTAAATCTTA ATTGATTTCA TTTTATTAAC 2951 ATATACCCTT TACCTTTAAT ATTTCATTTG AAGTGTTCCT TTCAAACTTA 3001 CTGTCTTAAA TATGAAAGTC AGCTTTAAGT AATGTCAGAC TCATATGCAT 3051 TTTCATTCTC ATTAGCTAAA GTAAAATGTA AAATTATCTC AAATAGTTAC 3101 AAGTTTTGGA AATACAGTAT AAAACATGAA TGTAAAGTCT ATTATGTAAT 3151 ATGCTTATTT GTAATCCTAA TATATGAGGG TGACATTTTT AAGATTGTAT 3201 GTATGTGTCA ACCTCTTAAA TGTTTTCTGT GAAAAAAAAA AAAAAAAAAA 3251 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 3301 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 3351 AAA BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 2 ORF from 167 bp to 1396 bp; peptide length: 410 Category: known protein Classification: unclassified 1 MVESSRHNWS GLDKQSDIQN LNEERILALQ LCGWIKKGTD VDVGPFLNSL 51 VQEGEWERAA AVALFNLDIR RAIQILNEGA SSEKGDLNLN VVAMALSGYT 101 DEKNSLWREM CSTLRLQLNN PYLCVMFAFL TSETGSYDGV LYENKVAVRD 151 RVAFACKFLS DTQLNRYIEK LTNEMKEAGN LEGILLTGLT KDGVDLMESY 201 VDRTGDVQTA SYCMLQGSPL DVLKDERVQY WIENYRNLLD AWRFWHKRAE 251 FDIHRSKLDP SSKPLAQVFV SCNFCGKSIS YSCSAVPHQG RGFSQYGVSG 301 SPTKSKVTSC PGCRKPLPRC ALCLINMGTP VSSCPGGTKS DEKVDLSKDK 351 KLAQFNNWFT WCHNCRHGGH AGHMLSWFRD HAECPVSACT CKCMQLDTTG 401 NLVPAETVQP BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_7j8, frame 2 PIR:S45391 probable membrane protein YBL104c - yeast (Saccharomyces cerevisiae), N = 2, Score = 446, P = 4.5e-47 TREMBL:AC004982_1 gene: "WUGSC :H_DJ1159O04.1"; Homo sapiens PAC clone DJ1159O04 from 7p21-p22, complete sequence., N = 1, Score = 2038, P = 7.6e-211 >TREMBL:AC004982_1 gene: "WUGSC:H_DJ1159O04.1"; Homo sapiens PAC clone DJ1159O04 from 7p21-p22, complete sequence. Length = 379 HSPs: Score = 2038 (305.8 bits), Expect = 7.6e-211, P = 7.6e-211 Identities = 379/379 (100%), Positives = 379/379 (100%) Query: 1 MVESSRHNWSGLDKQSDIQNLNEERILALQLCGWIKKGTDVDVGPFLNSLVQEGEWERAA 60 MVESSRHNWSGLDKQSDIQNLNEERILALQLCGWIKKGTDVDVGPFLNSLVQEGEWERAA Sb j ct: 1 MVESSRHNWSGLDKQSDIQNLNEERILALQLCGWIKKGTDVDVGPFLNSLVQEGEWERAA 60 Query: 61 AVALFNLDIRRAIQILNEGASSEKGDLNLNVVAMALSGYTDEKNSLWREMCSTLRLQLNN 120 AVALFNLDIRRAIQILNEGASSEKGDLNLNVVAMALSGYTDEKNSLWREMCSTLRLQLNN Sb j ct: 61 AVALFNLDIRRAIQILNEGASSEKGDLNLNVVAMALSGYTDEKNSLWREMCSTLRLQLNN 120 Query: 121 PYLCVMFAFLTSETGSYDGVLYENKVAVRDRVAFACKFLSDTQLNRYIEKLTNEMKEAGN 180 PYLCVMFAFLTSETGSYDGVLYENKVAVRDRVAFACKFLSDTQLNRYIEKLTNEMKEAGN Sbjct: 121 PYLCVMFAFLTSETGSYDGVLYENKVAVRDRVAFACKFLSDTQLNRYIEKLTNEMKEAGN 180 Query: 181 LEGILLTGLTKDGVDLMESYVDRTGDVQTASYCMLQGSPLDVLKDERVQYWIENYRNLLD 240 LEGILLTGLTKDGVDLMESYVDRTGDVQTASYCMLQGSPLDVLKDERVQYWIENYRNLLD Sbjct: 181 LEGILLTGLTKDGVDLMESYVDRTGDVQTASYCMLQGSPLDVLKDERVQYWIENYRNLLD 240 Query: 241 AWRFWHKRAEFDIHRSKLDPSΞKPLAQVFVSCNFCGKSISYSCΞAVPHQGRGFSQYGVSG 300 AWRFWHKRAEFDIHRSKLDPSSKPLAQVFVSCNFCGKSISYSCSAVPHQGRGFSQYGVSG Sbjct: 241 AWRFWHKRAEFDIHRSKLDPSSKPLAQVFVSCNFCGKSISYSCSAVPHQGRGFSQYGVSG 300 Query: 301 SPTKSKVTSCPGCRKPLPRCALCLINMGTPVSSCPGGTKSDEKVDLSKDKKLAQFNNWFT 360 SPTKSKVTSCPGCRKPLPRCALCLINMGTPVSSCPGGTKSDEKVDLSKDKKLAQFNNWFT Sbjct: 301 SPTKSKVTSCPGCRKPLPRCALCLINMGTPVSSCPGGTKSDEKVDLSKDKKLAQFNNWFT 360 Query: 361 WCHNCRHGGHAGHMLSWFR 379 WCHNCRHGGHAGHMLSWFR Sbjct: 361 WCHNCRHGGHAGHMLSWFR 379 Pedant information for DKFZphtes3_7j8, frame 2 Report for DKFZphtes3_7j8.2 [LENGTH] 410 [MW] 45862.45 [pi] 6.51 [HOMOL] TREMBL:AC004982_1 gene: "WUGSC :H_DJ1159O04.1"; Homo sapiens PAC clone DJ1159O04 from 7p21-p22, complete sequence. 0.0 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YBL104c] 7e-48 [BLOCKS] BL00028 Zinc finger, C2H2 type, domain proteins [BLOCKS] BL00534A Ferrochelatase proteins [PIRKW] transmembrane protein 2e-46 [KW] All Alpha SEQ MVESSRHNWSGLDKQSDIQNLNEERILALQLCGWIKKGTDVDVGPFLNSLVQEGEWERAA PRD cccccccccccccccccchhhhhhhhhhhhhhccccccccccccccccccccccchhhhh SEQ AVALFNLDIRRAIQILNEGAΞSEKGDLNLNVVAMALSGYTDEKNSLWREMCSTLRLQLNN PRD hhhhhhhhhhhhhhhhhcccchhhhhhhhhhhhhhhhhcccccchhhhhhhhhhhhhccc SEQ PYLCVMFAFLTSETGSYDGVLYENKVAVRDRVAFACKFLSDTQLNRYIEKLTNEMKEAGN PRD ccccceeeccccccccccceeeccchhhhhhhhhhhhhhcccchhhhhhhhhhhhhhhcc SEQ LEGILLTGLTKDGVDLMESYVDRTGDVQTASYCMLQGSPLDVLKDERVQYWIENYRNLLD PRD cceeeeeeccccchhhhhhhhcccccceeeeeccccccccccchhhhhhhhhhhhhhhhh SEQ AWRFWHKRAEFDIHRSKLDPSSKPLAQVFVSCNFCGKSIΞYSCSAVPHQGRGFSQYGVSG PRD hhhhhhhhhhhhhhcccccccccceeeeeeeccccccccccccccccccccccccccccc SEQ SPTKSKVTSCPGCRKPLPRCALCLINMGTPVSSCPGGTKSDEKVDLSKDKKLAQFNNWFT PRD ccccccccccccccccccceeeeecccccccccccccccccceeeehhhhhhhhhcceee SEQ WCHNCRHGGHAGHMLSWFRDHAECPVSACTCKCMQLDTTGNLVPAETVQP PRD eecccccccccchhhhhhhhhccccccccccccccccccccccccccccc (No Prosite data available for DKFZphtes3_7j8.2) (No Pfam data available for DKFZphtes3_7j8.2) DKFZphtes3_7plO group: Cell Cycle DKFZphtes3_7plO.1 encodes a novel 422 ammo ac d putative protein, which is closely related to the Xenopus laevis XPMC2 protein. In fission yeast the kinases Weel and Mikl control that initiation of mitosis starts after completion of DNA synthesis. Yeast in which both Weel and Mikl kinases are defective exhibit a mitotic catastrophe phenotype. XPMC2 of xenopus rescues several different yeast mitotic catastrophe mutants defective in Weel/Mikl kinase function. The XPMC2 protein is localised in the nucleus in Xenopus oocytes. The new protein is the human orthologue of this gene. The new protein can find application in modulating/blocking the cell cycle. strong similarity to XPMC2 protein complete cDMA, complete eds, EST hits Sequenced by BMFZ Locus: /map="9q34" Insert length: 2380 bp Poly A stretch at pos. 2341, polyadenylation signal at pos. 2318 1 AGCGTGCGTG CTGAGGTATG CGCAACGCGT GCGGGGTCTC TTCCGGAGTC 51 TTTTCCTGGA CGGGGTCCCT GCGGTGGGTG TGTTTCGGCC TGGCCTGGGC 101 AGGCGCTTGT GCTGCCAGGG CGCCGGGCCC GGGGAGGCCG GGGTCTCGGG 151 TGGCCGCCGG CCCAGGCGCT GGACGGCAGC AGGATGGGGA AGGCGAAGGT 201 CCCCGCCTCC AAGCGCGCCC CGAGCAGCCC CGTGGCTAAG CCGGGTCCTG 251 TCAAGACGCT CACTCGGAAG AAAAACAAGA AGAAAAAAAG GTTTTGGAAA 301 AGCAAGGCGC GGGAAGTAAG CAAGAAGCCA GCAAGCGGCC CCGGTGCTGT 351 GGTGCGACCT CCAAAGGCAC CAGAAGACTT TTCTCAAAAC TGGAAGGCGC 401 TGCAAGAGTG GCTGCTGAAA CAAAAATCTC AGGCCCCAGA AAAGCCTCTT 451 GTCATCTCTC AGATGGGTTC CAAAAAGAAG CCCAAAATTA TCCAGCAAAA 501 CAAAAAAGAG ACCTCGCCTC AAGTGAAGGG AGAGGAGATG CCGGCAGGAA 551 AAGACCAGGA GGCCAGCAGG GGCTCTGTTC CTTCAGGTTC CAAGATGGAC 601 AGGAGGGCGC CAGTACCTCG CACCAAGGCC AGTGGAACAG AGCACAATAA 651 GAAAGGAACC AAGGAAAGGA CAAATGGTGA TATTGTTCCA GAACGAGGGG 701 ACATCGAGCA TAAGAAGCGG AAAGCTAAGG AGGCAGCCCC AGCCCCACCC 751 ACCGAGGAAG ACATCTGGTT TGACGACGTG GACCCAGCGG ATATCGAAGC 801 TGCCATAGGT CCAGAGGCGG CCAAGATAGC GAGGAAACAG TTGGGTCAGA 851 GCGAGGGCAG CGTCAGCCTC AGCCTCGTGA AAGAGCAGGC CTTCGGCGGC 901 CTGACAAGAG CCTTAGCCTT GGACTGTGAG ATGGTGGGCG TGGGCCCTAA 951 GGGGGAGGAG AGCATGGCCG CCCGTGTGTC CATCGTGAAC CAGTATGGGA 1001 AGTGCGTTTA TGACAAGTAC GTCAAACCAA CTGAGCCCGT GACGGACTAT 1051 AGGACAGCGG TCAGTGGGAT TCGGCCTGAG AACCTCAAGC AGGGAGAAGA 1101 GCTTGAAGTT GTTCAGAAGG AAGTGGCAGA GATGCTGAAG GGCAGAATTC 1151 TAGTGGGGCA CGCTCTGCAT AATGACCTAA AGGTACTATT TCTTGATCAT 1201 CCAAAAAAGA AGATTCGGGA CACACAGAAA TATAAACCTT TCAAGAGTCA 1251 AGTAAAGAGT GGAAGGCCGT CTCTGAGACT ACTTTCAGAG AAGATCCTTG 1301 GGCTCCAGGT CCAGCAGGCG GAGCACTGTT CAATTCAGGA TGCCCAGGCA 1351 GCAATGAGGC TGTACGTCAT GGTGAAGAAG GAGTGGGAGA GCATGGCCCG 1401 AGACAGGCGC CCCCTGCTGA CTGCTCCAGA CCACTGCAGT GACGACGCCT 1451 AGCAGTCCTG CCCTGCTGCT GCTGCCGCCC CGCTACAGAG GCAATGTGAC 1501 CAGTCACAGG GACAGATCAC ATCTCCCCAG AGTGGCAACT CTGGTGAAAC 1551 CTTTTCAGAA TCATGGCAGA GGGGCGTGGC GTGGTGCTAC TGAGAAGGTC 1601 CTCCTTCCTC TTGACTTTGT GGTCTGAAAC CTGGTCTTAC TGTCCATGTG 1651 TGTTTGGGCC CGGATGGTCA GGGTGGGGAG CAGGGACGGC CATGGGCACG 1701 CCTGGCCACG CTTTACCGAC TGCTGACCCC CTGGGCCAGG TGAGGTTGGG 1751 GCCTGTGGGC CGCCAGTCCA TACGGTGCTG TCACTGCCCA TCTTCGGTGA 1801 CACCCTGGGG TGAGGTGCTC AGCACCTTCC TCTCGAGGAG CCACATTTTC 1851 CTCCTTTGTG TTAGGGGACA TAACAAGCTC TGCTGGGCTT GAGGGACCCA 1901 GACCAGGTGT CTGCAGTCAG CTCCTGAGAC ACAGCTGGCC GGCACAACAG 1951 GTGTTACATC AGGGGTTTCC TGTGGCCGTT TGAACTTTGA GCATTTATCT 2001 AAATTAAATT GGCCCAGGGT TGGCTGGTGG GTCACCCAGC AGAGGCTTCT 2051 CCCCATAGCA CGAGGATGTG TTGCCTGGGC ACGGTGACTG CGGTTATTCC 2101 TGGAGGTCGG CAGACATGCC AACCTTGGGC TATTTGAGCT GGAGAAGCTA 2151 TGTGATGCTA GCCGGTGGCT TTCTGGGCTA GGCCCCAGTT TGAGGCTCCC 2201 CTGGGAACTA GAGCCAGGAA CAGCCAGTGG CACTGACAAG GGGACGGAGT 2251 CCAAGGCGTT ATTGGGCCAC CTGACAGCTG GACAGAAAAG GGGCAGACAC 2301 ACCGAGGATG CGATTTAAAA TAAATGCAGA TGTTTACTTG GAAAAAAAAA 2351 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA BLAST Results Entry HSAC2099 from database EMBL: *** SEQUENCING IN PROGRESS *** Genomic sequence from Human 9q34; HTGS phase 1, 2 unordered pieces. Score = 5055, P = O.Oe+00, identities = 1011/1011 8 exons Bp 104219-116190 Medline entries 95157530: Cloning and expression of a Xenopus gene that prevents mitotic catastrophe in fission yeast. Peptide information for frame 1 ORF from 184 bp to 1449 bp; peptide length: 422 Category: strong similarity to known protein 1 MGKAKVPASK RAPSSPVAKP GPVKTLTRKK NKKKKRFWKS KAREVSKKPA 51 SGPGAVVRPP KAPEDFSQNW KALQEWLLKQ KSQAPEKPLV ISQMGSKKKP 101 KIIQQNKKET SPQVKGEEMP AGKDQEASRG SVPSGSKMDR RAPVPRTKAS 151 GTEHNKKGTK ERTNGDIVPE RGDIEHKKRK AKEAAPAPPT EEDIWFDDVD 201 PADIEAAIGP EAAKIARKQL GQSEGSVSLS LVKEQAFGGL TRALALDCEM 251 VGVGPKGEES MAARVSIVNQ YGKCVYDKYV KPTEPVTDYR TAVSGIRPEN 301 LKQGEELEVV QKEVAEMLKG RILVGHALHN DLKVLFLDHP KKKIRDTQKY 351 KPFKSQVKSG RPSLRLLSEK ILGLQVQQAE HCSIQDAQAA MRLYVMVKKE 401 WESMARDRRP LLTAPDHCSD DA BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_7pl0, frame 1 No Alert BLASTP hits found Pedant information for DKFZphtes3_7plO, frame 1 Report for DKFZphtes3_7plO .1 [LENGTH] 422 [MW] 46671.91 [pi] 9.79 [HOMOL] PIR:S53818 XPMC2 protein - African clawed frog 7e-96 [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YOL080c] 2e-42 [FUNCAT] 01.03.16 polynucleotide degradation [S. cerevisiae, YGR276c] 2e-19 [FUNCAT] 05.04 translation (initiation, elongation and termination) [Ξ . cerevisiae, YGL094C ] 7e-13 [FUNCAT] 04.05.05 mrna processing (5 '-end, 3 end processing and mrna degradation) [S. cerevisiae YGL094c] 7e-13 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YLR107w] 6e-10 [PROSITE] RGD 1 [PROSITE] MYRISTYL 4 [PROSITE] CAMP_PHOΞPHO_SITE 2 [PROSITE] CK2_PHOSPHO_SITE 6 [PROSITE] TYR_PHOSPHO_SITE 2 [PROSITE] GLYCOSAMINOGLYCAN 1 [PROSITE] PKC_PHOSPHO_SITE 8 [KW] All_Alpha [KW] LOW COMPLEXITY 11.37 % SEQ MGKAKVPASKRAPSSPVAKPGPVKTLTRKKNKKKKRFWKSKAREVSKKPASGPGAVVRPP SEG xxxxxxxxxxxxxxxxxx PRD cccccccccccccccccccccccccchhhhhhhhhhhhhhhhhccccccccccccccccc SEQ KAPEDFSQNWKALQEWLLKQKSQAPEKPLVISQMGΞKKKPKIIQQNKKETSPQVKGEEMP SEG xxxxxxxxxxxx PRD cccccccchhhhhhhhhhhhhhhcccccccccccccccccceeeecccccccccccccee SEQ AGKDQEASRGSVPSGΞKMDRRAPVPRTKASGTEHNKKGTKERTNGDIVPERGDIEHKKRK SEG xxxxxx PRD ecccccccccccccccccccccccccccccccccccccccccccccchhhhhhhhhhhhh SEQ AKEAAPAPPTEEDIWFDDVDPADIEAAIGPEAAKIARKQLGQSEGSVSLSLVKEQAFGGL SEG xxxxxxxxxxxx PRD hhhhcccccccceeeecccccchhhhhhccchhhhhhhhhhcccccchhhhhhhhhhhhh SEQ TRALALDCEMVGVGPKGEESMAARVSIVNQYGKCVYDKYVKPTEPVTDYRTAVSGIRPEN SEG PRD hhhcccccccccccccchhhhhhhhhccccccceeeeeeecccccccccccccccccccc SEQ LKQGEELEVVQKEVAEMLKGRILVGHALHNDLKVLFLDHPKKKIRDTQKYKPFKSQVKSG SEG PRD ccccchhhhhhhhhhhhhhcceeeeccchhhhhhhhhcccccccccceeecccccccccc SEQ RPSLRLLSEKILGLQVQQAEHCSIQDAQAAMRLYVMVKKEWESMARDRRPLLTAPDHCSD SEG PRD chhhhhhhhhhhhhhhccccccchhhhhhhhhhhhhhhhhhhhhhhhhcccccccccccc SEQ DA SEG PRD Prosite for DKFZphtes3_7plO .1 PS00002 51->55 GLYCOSAMINOGLYCAN PDOC00002 PS00004 107->111 CAMP_PHOSPHO_SITE PDOC00004 PS00004 156->160 CAMP_PHOΞPHO_SITE PDOC00004 PS00005 9->12 PKC_PHOSPHO_SITE PDOC00005 PS00005 27->30 PKC_PHOSPHO_SITE PDOC00005 PS00005 46->49 PKC_PHOSPHO_SITE PDOC00005 PS00005 96->99 PKC_PHOSPHO_SITE PDOC00005 PS00005 347->350 PKC_PHOSPHO_SITE PDOC00005 PS00005 359->362 PKC_PHOSPHO_SITE PDOC00005 PS00005 363->366 PKC_PHOSPHO_SITE PDOC00005 PS00005 368->371 PKC_PHOSPHO_SITE PDOC00005 PS00006 136->140 CK2_PHOSPHO_SITE PDOC00006 PΞ00006 150->154 CK2_PHOSPHO_SITE PDOC00006 PS00006 163->167 CK2_PHOSPHO_SITE PDOC00006 PS00006 190->194 CK2_PHOSPHO_SITE PDOC00006 PS00006 383->387 CK2_PHOSPHO_SITE PDOC00006 PS00006 413->417 CK2_PHOSPHO_SITE PDOC00006 PS00007 343->351 TYR_PHOSPHO_SITE PDOC00007 PS00007 342->351 TYR_PHOSPHO_SITE PDOC00007 PS00008 130->136 MYRISTYL PDOC00008 PS00008 151->157 MYRISTYL PDOC00008 PS00008 221->227 MYRISTYL PDOC00008 PS00008 239->245 MYRISTYL PDOC00008 PS00016 171->174 RGD PDOC00016 (No Pfam data available for DKFZphtes3_7plO .1) DKFZphtes3_7p9 group: nucleic acid management DKFZphtes3_7p9 encodes a novel 691 amino acid protein with similarity to human nuclear domain 10 protein NDP52. The nuclear domain (ND)10 also described as POD or Kr bodies is involved in the development of acute promyelocytic leukemia and virus-host interactions. The NDP52 protein is part of this complex structure. In vivo, NDP52 is transcribed in all human tissues, but is redistributed upon viral infection and interferon treatment. ND10 plays an important role in the viral life cycle. The novel protein is similar to NDP52. It contains three leucine zippers and a RGD cell attachment site. This protein seems to be a novel part of the ND819) complex. The new protein can find application in modulation of viral infections and tumour events. similarity to nuclear domain 10 protein NDP52 complete cDNA, complete eds, EST hits Sequenced by BMFZ Locus: /map="329.1 cR from top of Chrl2 linkage group" Insert length: 3003 bp Poly A stretch at pos. 2957, no polyadenylation signal found 1 AAGGTGAGGG GAACAGCTGA TCCGTCTGTT GGGAGGACAG ATATCTCAAG 51 GCCAGGATGG AAGAATCACC ACTAAGCCGG GCACCATCCC GTGGTGGAGT 101 CAACTTTCTC AATGTAGCCC GGACCTACAT CCCCAACACC AAGGTGGAAT 151 GTCACTACAC CCTTCCCCCA GGCACCATGC CCAGTGCCAG TGACTGGATT 201 GGCATCTTCA AGGTGGAGGC TGCCTGTGTT CGGGATTACC ACACATTTGT 251 GTGGTCTTCC GTGCCTGAAA GTACAACTGA TGGTTCCCCC ATTCACACCA 301 GTGTCCAGTT CCAAGCCAGC TACCTGCCCA AACCAGGAGC TCAGCTCTAC 351 CAGTTCCGAT ATGTGAACCG CCAGGGCCAG GTGTGTGGGC AGAGCCCCCC 401 TTTCCAGTTC CGAGAGCCAA GGCCCATGGA TGAACTGGTG ACCCTGGAGG 451 AGGCTGATGG GGGCTCTGAC ATCCTGCTGG TTGTCCCCAA GGCAACTGTG 501 TTACAGAACC AGCTCGATGA GAGCCAGCAA GAACGGAATG ACCTGATGCA 551 GCTGAAGCTA CAGCTGGAGG GACAGGTGAC AGAGCTGAGG AGCCGAGTGC 601 AGGAGCTCGA GAGGGCTCTG GCAACTGCCA GGCAGGAGCA CACGGAGCTG 651 ATGGAACAGT ACAAGGGGAT TTCCCGGTCC CATGGGGAGA TCACAGAAGA 701 GAGGGACATC CTGAGCCGGC AACAGGGAGA CCATGTGGCA CGCATCCTGG 751 AGCTAGAGGA TGACATCCAG ACCATCAGTG AGAAAGTGCT GACGAAGGAA 801 GTGGAGCTGG ACAGGCTTAG AGACACAGTG AAGGCCCTGA CTCGGGAACA 851 AGAGAAGCTC CTTGGGCAAC TGAAAGAAGT ACAAGCAGAC AAGGAGCAAA 901 GTGAGGCTGA GCTCCAAGTG GCACAACAGG AGAACCATCA CTTAAATTTG 951 GACCTGAAGG AGGCGAAGAG CTGGCAAGAG GAGCAGAGTG CTCAGGCTCA 1001 GCGACTGAAA GACAAGGTGG CCCAGATGAA GGACACCCTA GGCCAGGCCC 1051 AGCAGCGGGT GGCCGAGCTG GAGCCCTTGA AGGAGCAGCT TCGAGGGGCC 1101 CAGGAGCTTG CAGCCTCAAG CCAGCAGAAA GCCACCCTTC TTGGGGAGGA 1151 GTTGGCCAGC GCAGCAGCAG CCAGGGACCG CACCATAGCC GAACTACACC 1201 GCAGCCGCCT GGAAGTGGCT GAAGTTAACG GCAGGCTGGC TGAGCTCGGT 1251 TTGCACTTGA AGGAAGAAAA ATGCCAATGG AGCAAGGAGC GGGCAGGGCT 1301 GCTGCAGAGT GTGGAGGCAG AGAAGGACAA GATCCTGAAG CTGAGTGCAG 1351 AGATACTTCG ATTGGAGAAG GCAGTTCAGG AGGAGAGGAC CCAAAACCAA 1401 GTGTTCAAGA CTGAGCTGGC CCGGGAGAAG GATTCTAGCC TGGTACAGTT 1451 GTCAGAAAGT AAGCGGGAGC TGACAGAGCT GCGGTCAGCC CTGCGTGTGC 1501 TCCAGAAGGA AAAGGAGCAG TTACAGGAGG AGAAACAGGA ATTGCTAGAG 1551 TACATGAGAA AGCTAGAGGC CCGCCTGGAG AAGGTGGCAG ATGAGAAGTG 1601 GAATGAGGAT GCCACCACAG AGGATGAGGA GGCCGCTGTG GGGCTGAGCT 1651 GCCCGGCAGC TCTGACAGAC TCAGAGGACG AGTCCCCAGA AGACATGAGG 1701 CTCCCACCCT ATGGCCTTTG TGAGCGTGGA GACCCAGGCT CCTCTCCTGC 1751 TGGGCCTCGA GAGGCTTCTC CCCTTGTTGT CATCAGCCAG CCGGCTCCCA 1801 TTTCTCCTCA CCTCTCTGGG CCAGCTGAGG ACAGTAGCTC TGACTCGGAG 1851 GCTGAAGATG AGAAGTCAGT CCTGATGGCA GCTGTGCAGA GTGGGGGTGA 1901 GGAGGCCAAC TTACTGCTTC CTGAACTGGG CAGTGCCTTC TATGACATGG 1951 CCAGTGGCTT TACAGTGGGT ACCCTGTCAG AAACCAGCAC TGGGGGCCCT 2001 GCCACCCCCA CATGGAAGGA GTGTCCTATC TGTAAGGAGC GCTTTCCTGC 2051 TGAGAGTGAC AAGGATGCCC TGGAGGACCA CATGGATGGA CACTTCTTTT 2101 TCAGCACCCA GGACCCCTTC ACCTTTGAGT GATCTTACTC CCTCGTACAT 2151 GCACAAATAC ACACTCATGC ACACACACAC TCACACACAT GCATACACTT 2201 AGGTTTCATG CCCATTTTCT ATCACACTGG GCTCCATGAT ATTCTGTTCC 2251 CTAAGAACTG CTTCTGTGTG CCCTGTTTTC ATCCCAAGAT TTCTCACTTC 2301 ATCCTCTCCT ACCTGGCTCT TTTGTCCCAG GGAGGGGTCC TGTTCGGAAG 2351 CAGTGGCTGA ATTTATCCCC TGAAAGTGGT TTTGGAGGAA CCGGGATGGA 2401 GGAGGCCTTC CCCTGTGGGA ATAGAATCGT CCACTCCTAG CCCTGGTTGC 2451 TTCTGATACA CAGCCACTGC ACACACACAC TCACACTCAC ACTCCCTTGT 2501 CTGATGCCCC AAAGCCAATT CCTGGGGCAC CCTACCCTCT CTTATTTGGA 2551 GTTTCCGTTG GTTTACCTGA GTTTTCTCTG GGGTCTGCAC AGAGGCAGCA 2601 GCATGGACAT CATGGCCTCT CAGGTCCCTT TTGGTTCTCA GTTTCATTGG 2651 TTCCTCTTTC TGTTCCCCCA TTGACTTCTG TGCCCCACCC TAGCCTTTTC 2701 CATAACCTTA GGTATTCAGT TTGGAGGGGT TTTTTGTATT TTTGAGGATT 2751 CCTGTATTCT GTATCCTCTC CTCGCATCTC CTCACATGGA AAGAAATAAT 2801 GTATTTGTGC CTTCTGTGAG GAATGGGGGG AACAAGTGGT CCCAGGTATC 2851 CCCATTTCCA AGGCCCCCCT CCCTCTCCAG GTCCCCCCAC AGCAATAAAA 2901 GCTTCCCCCT GATATCCATC CCTTTGTAGT TTGAACAAAT ATATTTATAT 2951 GATATGTAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 3001 AAA BLAST Results Entry HS189353 from database EMBL: human STS WI-11261. Score = 2191, P = 1.4e-92, identities = 463/485 Medline entries 95310349: Molecular characterization of NDP52, a novel protein of the nuclear domain 10, which is redistributed upon virus infection and interferon treatment. 97375672: Cellular localization, expression, and structure of the nuclear dot protein 52. Peptide information for frame 3 ORF from 57 bp to 2129 bp; peptide length: 691 Category: similarity to known protein Prosite motifs: RGD (557-560) LEUCINE_ZIPPER (163-185) LEUCINE_ZIPPER (475-497) LEUCINE ZIPPER (482-504) 1 MEESPLSRAP SRGGVNFLNV ARTYIPNTKV ECHYTLPPGT MPSASDWIGI 51 FKVEAACVRD YHTFVWSSVP ESTTDGSPIH TSVQFQASYL PKPGAQLYQF 101 RYVNRQGQVC GQSPPFQFRE PRPMDELVTL EEADGGSDIL LVVPKATVLQ 151 NQLDESQQER NDLMQLKLQL EGQVTELRSR VQELERALAT ARQEHTELME 201 QYKGISRSHG EITEERDILS RQQGDHVARI LELEDDIQTI SEKVLTKEVE 251 LDRLRDTVKA LTREQEKLLG QLKEVQADKE QSEAELQVAQ QENHHLNLDL 301 KEAKSWQEEQ SAQAQRLKDK VAQMKDTLGQ AQQRVAELEP LKEQLRGAQE 351 LAASSQQKAT LLGEELASAA AARDRTIAEL HRSRLEVAEV NGRLAELGLH 401 LKEEKCQWSK ERAGLLQSVE AEKDKILKLS AEILRLEKAV QEERTQNQVF 451 KTELAREKDS SLVQLSESKR ELTELRSALR VLQKEKEQLQ EEKQELLEYM 501 RKLEARLEKV ADEKWNEDAT TEDEEAAVGL SCPAALTDSE DESPEDMRLP 551 PYGLCERGDP GSSPAGPREA SPLVVISQPA PISPHLSGPA EDSSSDSEAE 601 DEKSVLMAAV QSGGEEANLL LPELGSAFYD MASGFTVGTL SETSTGGPAT 651 PTWKECPICK ERFPAESDKD ALEDHMDGHF FFSTQDPFTF E BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_7p9, frame 3 PIR:A56733 nuclear domain 10 protein NDP52 - human, N = 2, Score = 307, P = 7.7e-28 TREMBL :AB008852_1 gene: "NDP"; product: "NDP52"; Bos taurus mRNA for NDP52, complete eds., N = 2, Score = 302, P = 4e-27 TREMBL :AC004549_1 gene: "WUGSC :H_RG459N13.1"; product: "TXBP151"; Homo sapiens BAC clone RG459N13 from 7pl5, complete sequence., N = 2, Score = 275, P = 2.3e-25 PIR:G02043 TXBP151 - human, N = 2, Score = 270, P = 8.5e-25 TREMBL: DM35816_4 gene: "zip"; product: "nonmuscle myosin-II heavy chain"; Drosophila melanogaster nonmuscle myosin-II heavy chain (zip) gene, complete eds., N = 1, Score = 254, P = 1.4e-17 >PIR:A56733 nuclear domain 10 protein NDP52 - human Length = 446 HSPs: Score = 307 (46.1 bits), Expect = 7.7e-28, Sum P(2) = 7.7e-28 Identities = 104/323 (32%), Positives = 158/323 (48%) Query: 15 VNFLNVARTYIPNTKVECHYTLPPGTMPSASDWIGIFKVEAACVRDYHTFVWSSVPESTT 74 V F +V + YIP V CHYT +P DWIGIF+V R+Y+TF+W ++P Sbjct: 23 VIFNSVEKFYIPGGDVTCHYTFTQHFIPRRKDWIGIFRVGWKTTREYYTFMWVTLPIDLN 82 Query: 75 DGSPIHTSVQFQASYLPKPGAQLYQFRYVNRQGQVCGQSPPFQFREPRPMDELVTLEEAD 134 + S VQF+A YLPK + YQF YV+ G V G S PFQFR D LV + Sbjct: 83 NKSAKQQEVQFKAYYLPKDD-EYYQFCYVDEDGVVRGASIPFQFRPENEEDILVVTTQ— 139 Query: 135 GGSDILLVVPKATVLQNQ-LDES QQERNDLMQLKLQLEGQVTE-LRSRVQELERALA 189 G + + K +NQ L +S Q++N MQ +LQ + + E L+S ++LE + Sbjct: 140 GEVEEIEQHNKELCKENQELKDSCISLQKQNSDMQAELQKKQEELETLQSINKKLELKVK 199 Query: 190 TARQE-HTELMEQYKGISRSHGEITEERDI-LSRQQGDHVARILELEDDIQTISEKVLTK 247 + TEL+ Q K ++ E+ I + + Q + E+E +Q +K T+ Sbjct: 200 EQKDYWETELL-QLKEQNQKMSΞENEKMGIRVDQLQAQLSTQEKEMEKLVQGDQDK—TE 256 Query: 248 EVE-LDRLRDTVKALTREQEKLLGQLKEVQADKEQSEAELQVAQQENHHLNLDLKEAKSW 306 ++E L + D + EQ K +L++ +Q+E QQE N DL + S Sbjct: 257 QLEQLKKENDHLFLSLTEQRKDQKKLEQTVEQMKQNETTAMKKQQELMDENFDLSKRLSE 316 Query: 307 QEEQSAQAQRLKDKVAQMKDTLGQAQQRV 335 E QR K+++ D L + R+ Sbjct: 317 NEIICNALQRQKERLEGENDLLKRENSRL 345 Score = 304 (45.6 bits), Expect = 2. le-27, Sum P(2) = 2. le-27 Identities = 98/337 (29%), Positives = 163/337 (48%) Query: 15 VNFLNVARTYIPNTKVECHYTLPPGTMPSASDWIGIFKVEAACVRDYHTFVWSSVPESTT 74 V F +V + YIP V CHYT +P DWIGIF+V R+Y+TF+W ++P Sbjct: 23 VIFNSVEKFYIPGGDVTCHYTFTQHFIPRRKDWIGIFRVGWKTTREYYTFMWVTLPIDLN 82 Query: 75 DGSPIHTSVQFQASYLPKPGAQLYQFRYVNRQGQVCGQSPPFQFREPRPMDELVTLEEAD 134 + S VQF+A YLPK + YQF YV+ G V G S PFQFR P +E Sbjct: 83 NKSAKQQEVQFKAYYLPKDD-EYYQFCYVDEDGVVRGASIPFQFR PENE 130 Query: 135 GGSDILLVVPKATVLQNQLDESQQERNDLMQLKLQLEGQVTELRSRVQELERALATARQE 194 DIL+V Q +++E +Q +L + +L+ L+ + +++ L +QE Sbjct: 131 --EDILVVTT QGEVEEIEQHNKELCKENQELKDSCISLQKQNSDMQAELQK-KQE 182 Query: 195 HTELMEQYKGISRSHGEITEERDILSRQQGDH-VARILELEDDIQTISEKVLTKEVELDR 253 E ++ I ++ ++ ++Q D+ +L+L++ Q +S + + +D+ Sbjct: 183 ELETLQS INKKLELKVKEQKDYWETELLQLKEQNQKMSSENEKMGIRVDQ 232 Query: 254 LRDTVKALTREQEKLL—GQLKEVQAD KEQSEAELQVAQQENHHLNLDLKEAKSWQE 308 L+ + +E EKL+ Q K Q + KE L + +Q L+ + Q Sbjct: 233 LQAQLSTQEKEMEKLVQGDQDKTEQLEQLKKENDHLFLSLTEQRKDQKKLEQTVEQMKQN 292 Query: 309 EQSA—QAQRLKDKVAQMKDTLGQAQQRVAELEPLKEQLRGAQEL 351 E +A + Q L D+ + L + + L+ KE+L G +L Sbjct: 293 ETTAMKKQQELMDENFDLSKRLSENEIICNALQRQKERLEGENDL 337 Score = 124 (18.6 bits), Expect = 2.3e-06, Sum P(2) = 2.3e-06 Identities = 53/227 (23%), Positives = 113/227 (49%) Query: 138 DILLVVPKATVLQNQLDESQQERNDLMQLKLQLEGQVTELRSRVQELERALATARQEHTE 197 DIL+V Q +++E +Q +L + +L+ L+ + +++ L +QE E Sbjct: 132 DILVVTT QGEVEEIEQHNKELCKENQELKDSCISLQKQNSDMQAELQK-KQEELE 185 Query: 198 LMEQYKGISRSHGEITEERDILSRQQGDH-VARILELEDDIQTISEKVLTKEVELDRLRD 256 ++ I ++ ++ ++Q D+ +L+L++ Q +S + + +D+L+ Sbjct: 186 TLQS INKKLELKVKEQKDYWETELLQLKEQNQKMSΞENEKMGIRVDQLQA 235 Query: 257 TVKALTREQEKLLGQLKEVQADKEQSEAELQVAQQENHHLNLDLKEAKSWQEEQSAQAQR 316 + +E EKL VQ D++++E +L+ ++EN HL L L E + Q++ ++ Sbjct: 236 QLSTQEKEMEKL VQGDQDKTE-QLEQLKKENDHLFLSLTEQRKDQKKLEQTVEQ 288 Query: 317 LK-DKVAQMKDTLGQAQQRVAELEPLKEQLRGAQELA-ASSQQKATLLGE 364 +K ++ MK + Q+ + E L ++L + + A +QK L GE Sb j ct: 289 MKQNETTAMK KQQELMDENFDLSKRLSENEIICNALQRQKERLEGE 334 Score = 103 (15.5 bits), Expect = 4.4e-04, Sum P(2) = 4.4e-04 Identities = 63/278 (22%), Positives = 123/278 (44%) Query: 299 DLKEAKSWQEEQSAQAQRLKDKVAQMK DTLGQAQQRVAELEPLKEQLRGAQELAAS 354 +++E + +E + Q LKD ++ D + Q++ ELE L + + EL Sbjct: 141 EVEEIEQHNKELCKENQELKDSCISLQKQNSDMQAELQKKQEELETL-QSINKKLELKVK 199 Query: 355 SQQKATLLGEELASAAAARDRTIAELHRSRLEVAEVNGRLAELGLHLKEEKCQWSKERAG 414 Q+ EL + +E + + V ++ +L+ + E+ Q +++ Sbjct: 200 EQKD—YWETELLQLKEQNQKMΞSENEKMGIRVDQLQAQLSTQEKEM-EKLVQGDQDKTE 256 Query: 415 LLQSVEAEKDKI-LKLSAEIL RLEKAVQEERTQNQVFKTELAREKDSSLVQLSESKR 470 L+ ++ E D + L L+ + +LE+ V E+ QN+ T + ++++ SKR Sbjct: 257 QLEQLKKENDHLFLSLTEQRKDQKKLEQTV-EQMKQNET--TAMKKQQELMDENFDLSKR 313 Query: 471 ELTELRSALRVLQKEKEQLQEEKQELLEYMRKLEARLEKVADEKWNE DATTEDEEAA 527 L+E LQ++KE+L+ E +LL ++ +RL +N T DE A Sbjct: 314 -LSENEIICNALQRQKERLEGEN-DLL KRENSRLLSYMGLDFNSLPYQVPTSDEGGA 368 Query: 528 VGLSCPAALTD-SEDESPEDMRLPPYGLCERGDPGSSPAGPREASPL 573 GL+ + E SP + + +C+ D ++ PL Sbjct: 369 RQNPGLAYGNPYSGIQESSSPSPLSIKKCPICKADDICDHTLEQQQMQPL 418 Score = 64 (9.6 bits), Expect = 7.7e-28, Sum P(2) = 7.7e-28 Identities = 13/29 (44%), Positives = 17/29 (58%) Query: 651 PTWKECPICKERFPAESDKDALEDHMDGH 679 P CPIC + FPA ++K EDH+ H Sbjct: 417 PLCFNCPICDKIFPA-TEKQIFEDHVFCH 444 Score = 64 (9.6 bits), Expect = 5.8e+00, Sum P(2) l.Oe+00 Identities = 26/90 (28%), Positives = 45/90 (50%) Query: 470 RELTELRSALRVLQKEKEQLQEE KQELLEYMRKLEARLE-KVADEK—W 515 +E EL+ + LQK+ +Q E KQE LE ++ + +LE KV ++K W Sbjct: 154 KENQELKDSCISLQKQNSDMQAELQKKQEELETLQSINKKLELKVKEQKDYWETELLQLK 213 Query: 516 —NEDATTEDEEAAVGLS-CPAALTDSEDE 542 N+ ++E+E+ + + A L+ E E Sbjct: 214 EQNQKMSSENEKMGIRVDQLQAQLSTQEKE 243 Score = 47 (7.1 bits), Expect = 4.6e-26, Sum P(2) 4.6e-26 Identities = 11/30 (36%), Positives = 17/30 (56%) Query: 631 MASGFTVGTLSETSTGGPATPTWKECPICK 660 +A G + E+S+ P + K+CPICK Sbjct: 374 LAYGNPYSGIQESSSPSPLSI—KKCPICK 401 Pedant information for DKFZphtes3_7p9, frame 3 Report for DKFZphtes3_7p9.3 [LENGTH] 691 [MW] 77336.52 [pi] 4.77 [HOMOL] PIR:A56733 nuclear domain 10 protein NDP52 - human 2e-29 [FUNCAT] 09.10 nuclear biogenesis [S. cerevisiae, YDR356w] 2e-ll [FUNCAT] 30.04 organization of cytoskeleton [S. cerevisiae, YDR356w] 2e-ll [FUNCAT] 08.07 vesicular transport (golgi network, etc.) [S. cerevisiae, YDL058w] 2e-ll [FUNCAT] 03.22 cell cycle control and mitosis [S. cerevisiae, YDR356w] 2e-ll [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YDL058w] 2e-ll [FUNCAT] 99 unclassified proteins [S. cerevisiae, YLR309c] 2e-08 [FUNCAT] 03.04 budding, cell polarity and filament formation [S. cerevisiae, YHR023w MYOl - myosm-1 isoform] 3e-07 [FUNCAT] 08.22 cytoskeleton-dependent transport [S. cerevisiae, YHR023w MYOl - myosm-1 isoform] 3e-07 [FUNCAT] 03.25 cytokinesis [S. cerevisiae, YHR023w MYOl - myosιn-1 isoform] 3e-07 [FUNCAT] 09.13 biogenesis of chromosome structure [S. cerevisiae, YJL074c] 4e-07 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YNL250w] 4e-06 [FUNCAT] 03.07 pheromone response, mating-type determination, sex-specific proteins [S. cerevisiae, YBR289w] 4e-06 [FUNCAT] 01.05.04 regulation of carbohydrate utilization [S. cerevisiae, YBR289w] 4e-06 [FUNCAT] 04.05.01.04 transcriptional control [S. cerevisiae, YBR289w] 4e-06 [FUNCAT] 03.19 recombination and dna repair [S. cerevisiae, YNL250w] 4e-06 [FUNCAT] 03.13 meiosis [S. cerevisiae, YNL250w] 4e-06 [FUNCAT] 1 genome replication, transcription, recombination and repair [M. jannaschii, MJ1643] le-05 [FUNCAT] 98 classification not yet clear-cut [S. cerevisiae, YJR134c] 4e-05 [FUNCAT] 11.04 dna repair (direct repair, base excision repair and nucleotide excision repair) [S. cerevisiae, YKR095w] 4e-05 [ FUNCA ] 08.19 cellular import [S. cerevisiae, YNL243w] 7e-05 [ FUNCAT ] 01.03.16 polynucleotide degradation [S. cerevisiae, YNL243w] 7e-05 [FUNCAT] 06.10 assembly of protein complexes [S. cerevisiae, YNL243w] 7e-05 [FUNCAT] 08.99 other intracellular-transport activities [Ξ. cerevisiae, YNL079c] 2e-04 [FUNCAT] 03.01 cell growth [S. cerevisiae, YNL079c] 2e-04 [BLOCKS] BL00682B ZP domain proteins [EC] 3.6.1.32 Myosin ATPase le-13 [PIRKW] nucleus 6e-10 [PIRKW] phosphotransferase 2e-07 [PIRKW] duplication 9e-07 [PIRKW] citrulline le-09 [PIRKW] tandem repeat le-13 [PIRKW] heart 5e-ll [PIRKW] endocytosis 5e-09 [PIRKW] polymorphism 3e-06 [PIRKW] cormfied cell envelope le-06 [PIRKW] transmembrane protein 6e-12 [PIRKW] serine/threomne-specific protein kinase 2e-07 [PIRKW] cell wall le-06 [PIRKW] zinc finger 5e-09 [PIRKW] metal binding 5e-09 [PIRKW] DNA binding 8e-08 [PIRKW] muscle contraction le-11 [PIRKW] IgG constant region-binding le-06 [PIRKW] acetylated amino end 4e-09 [PIRKW] actin binding le-13 [PIRKW] mitosis 9e-09 [PIRKW] microtubule binding 9e-09 [PIRKW] ATP le-13 [PIRKW] thick filament le-10 [PIRKW] phosphoprotem le-13 [PIRKW] epidermis le-06 [PIRKW] leucine zipper le-07 [PIRKW] glycoprotein 4e-07 [PIRKW] skeletal muscle 4e-10 [PIRKW] disulfide bond le-07 [PIRKW] calcium binding le-09 [PIRKW] alternative splicing le-10 [PIRKW] coiled coil le-13 [PIRKW] P-loop le-13 [PIRKW] heptad repeat 6e-10 [PIRKW] methylated amino acid le-13 [PIRKW] basement membrane 3e-06 [PIRKW] immunoglobulin receptor 2e-07 [PIRKW] peripheral membrane protein 5e-09 [PIRKW] dimer le-07 [PIRKW] cardiac muscle le-10 [PIRKW] extracellular matrix 3e-06 [PIRKW] hydrolase le-13 [PIRKW] microtubule 6e-10 [PIRKW] muscle 2e-09 [PIRKW] membrane protein 3e-06 [PIRKW] EF hand le-09 [PIRKW] cytoskeleton 6e-12 [PIRKW] hair le-09 [PIRKW] calmodulin binding 5e-09 [PIRKW] Golgi apparatus 3e-08 [SUPFAM] myosin heavy chain le-13 [SUPFAM] conserved hypothetical P115 protein le-08 [SUPFAM] hypothetical protein YJL074c 5e-07 [SUPFAM] centromere protein E 9e-09 [SUPFAM] unassigned Ser/Thr or Tyr-specific protein kinases 2e-07 [SUPFAM] calmodulin repeat homology le-09 [SUPFAM] myosin motor domain homology le-13 [SUPFAM] alpha-actinin actm-binding domain homology 3e-13 [SUPFAM] tropomyosin 3e-07 [SUPFAM] plectin 3e-13 [SUPFAM] trichohyalm le-09 [SUPFAM] pleckstrm repeat homology 4e-06 [SUPFAM] ribosomal protein S10 homology 3e-13 [SUPFAM] giantin 3e-08 [SUPFAM] protein kinase homology 2e-07 [SUPFAM] protein kinase C z c-binding repeat homology 4e-06 [SUPFAM] involucrin le-06 [SUPFAM] kinesin motor domain -homology 9e-09 [SUPFAM] human early endosome antigen 1 5e-09 [SUPFAM] unassigned kinesin-related proteins 8e-08 [SUPFAM] M5 protein 3e-08 [SUPFAM] cytoskeletal keratin 3e-08 [PROSITE] LEUCINE_ZIPPER 3 [PROSITE] RGD 1 [PROSITE] MYRISTYL 6 [PROSITE] CK2_PHOSPHO_SITE 25 [PROSITE] PKC_PHOSPHO_SITE 6 [KW] All_Alpha [KW] LOW_COMPLEXITY 9.12 % [KW] COILED COIL 39.36 % SEQ MEESPLSRAPSRGGVNFLNVARTYIPNTKVECHYTLPPGTMPSASDWIGIFKVEAACVRD SEG PRD cccccccccccccceeeecceeeeeccccceeeeeccccccccccceeeeeeeeeecccc COILS SEQ YHTFVWSSVPESTTDGSPIHTSVQFQASYLPKPGAQLYQFRYVNRQGQVCGQSPPFQFRE SEG PRD eeeeeeeecccccccccchhhhhhhhhhhhccccccceeeeecccccccccccccccccc COILS SEQ PRPMDELVTLEEADGGSDILLVVPKATVLQNQLDESQQERNDLMQLKLQLEGQVTELRSR SEG PRD cccccceeehhhhhchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC SEQ VQELERALATARQEHTELMEQYKGISRSHGEITEERDILSRQQGDHVARILELEDDIQTI SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCC SEQ ΞEKVLTKEVELDRLRDTVKALTREQEKLLGQLKEVQADKEQSEAELQVAQQENHHLNLDL SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC SEQ KEAKSWQEEQSAQAQRLKDKVAQMKDTLGQAQQRVAELEPLKEQLRGAQELAASSQQKAT SEG XX PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCC .. CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC . CCCCCCCCCCCCCCCCCCCC SEQ LLGEELASAAAARDRTIAELHRSRLEVAEVNGRLAELGLHLKEEKCQWSKERAGLLQSVE SEG xxxxxxxxxxxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCC CCCCCCCCCCC SEQ AEKDKILKLΞAEILRLEKAVQEERTQNQVFKTELAREKDSSLVQLSESKRELTELRSALR SEG PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCC CCCCCCCCCCCCCCCCCCC SEQ VLQKEKEQLQEEKQELLEYMRKLEARLEKVADEKWNEDATTEDEEAAVGLSCPAALTDSE SEG . xxxxxxxxxxxxxxxxx xxxxxxxxxxx PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC SEQ DESPEDMRLPPYGLCERGDPGSSPAGPREASPLVVISQPAPISPHLSGPAEDSSSDSEAE SEG xxxxxxxxxxx PRD hhhhccccccccccccccccccccccccccceeeeeeccccccccccccccccccccchh COILS SEQ DEKSVLMAAVQSGGEEANLLLPELGSAFYDMASGFTVGTLSETSTGGPATPTWKECPICK SEG xx PRD hhhhhhhhhhhhcccccccccccccccccccccccccccccccccccccccccccccccc COILS SEQ ERFPAESDKDALEDHMDGHFFFSTQDPFTFE SEG PRD cccccccchhhhhhhccccceeecccccccc COILS DKFZphtes3_8e24 group: signal transduction DKFZphtes3_8e24.3 encodes a novel 658 amino acid putative GTP-binding protein, related to yeast YGL099w and mouse MMR1 putative GTP-binding proteins. GTP-bmding proteins are involved m various signal transduction pathways, transferring the signal of a cellular receptor to an intracellular signal cascade. The new protein can find clinical application in modulating/blocking the response to a cellular receptor. strong similarity to guanine nucleotide binding proteins complete cDNA, complete eds, potential start at Bp 31, EST hits Sequenced by MediGenomix Locus: unknown Insert length: 3290 bp Poly A stretch at pos. 3269, polyadenylation signal at pos. 3251 1 CGTCCAGCGG TCGTGTTGCC ATGGGCCGGA GGAGAGCCCC GGCCGGTGGG 51 TCGCTGGGAC GGGCCCTTAT GCGCCATCAG ACTCAGCGGA GCCGAAGCCA 101 TCGTCACACT GACTCCTGGT TGCACACAAG TGAACTCAAT GATGGCTATG 151 ATTGGGGTCG TCTTAATCTT CAGTCAGTGA CTGAACAGAG CTCCCTTGAT 201 GACTTCCTTG CTACTGCAGA ACTTGCAGGA ACAGAGTTTG TAGCTGAAAA 251 ACTTAATATT AAGTTTGTGC CTGCTGAGGC TAGAACTGGA CTACTGTCTT 301 TCGAGGAGAG CCAGAGAATT AAGAAGCTCC ATGAAGAAAA CAAACAGTTC 351 TTGTGTATAC CGAGGAGACC AAACTGGAAC CAAAATACTA CCCCAGAAGA 401 ACTCAAACAA GCAGAGAAAG ATAACTTTCT AGAATGGAGA CGTCAGCTTG 451 TCCGGCTAGA AGAGGAACAG AAGCTGATAT TGACTCCATT TGAACGAAAT 501 TTGGACTTTT GGCGCCAGCT CTGGAGAGTC ATTGAGAGAA GTGATATTGT 551 GGTCCAGATA GTAGATGCTC GAAACCCACT CCTGTTTAGA TGTGAGGATT 601 TGGAATGTTA TGTGAAAGAA ATGGATGCCA ATAAGGAGAA CGTCATTCTG 651 ATCAACAAGG CAGACTTGCT GACTGCTGAG CAGCGGAGTG CCTGGGCCAT 701 GTACTTCGAA AAAGAAGATG TGAAGGTTAT TTTCTGGTCA GCTTTGGCCG 751 GAGCCATTCC CCTGAATGGT GACTCTGAGG AAGAGGCAAA CAGAGATGAT 801 AGACAAAGCA ACACAACTGA GTTTGGACAT TCCAGTTTCG ACCAGGCTGA 851 AATTTCCCAC AGTGAATCCG AACATCTCCC AGCTAGGGAT TCTCCTTCAC 901 TTAGTGAAAA TCCCACAACG GATGAAGATG ACAGTGAGTA TGAGGACTGT 951 CCAGAGGAGG AGGAAGACGA CTGGCAGACG TGCTCAGAAG AAGACGGTCC 1001 CAAGGAAGAG GACTGCAGCC AGGACTGGAA GGAAAGCTCT ACTGCAGATT 1051 CTGAGGCTCG GAGCAGGAAA ACCCCACAGA AGAGGCAGAT ACACAATTTT 1101 AGCCATCTGG TATCCAAGCA GGAGTTACTG GAGCTCTTTA AGGAGCTACA 1151 CACTGGGAGA AAGGTGAAAG ATGGGCAACT TACGGTCGGA CTGGTGGGCT 1201 ACCCTAATGT TGGTAAGAGT TCAACAATCA ACACCATCAT GGGCAACAAG 1251 AAAGTATCTG TGTCTGCCAC ACCTGGTCAC ACAAAGCACT TTCAGACTCT 1301 CTATGTGGAG CCTGGCCTCT GCCTGTGTGA CTGTCCTGGC TTGGTGATGC 1351 CATCTTTTGT GTCTACCAAG GCAGAAATGA CTTGCAGCGG AATCCTCCCA 1401 ATTGATCAGA TGAGAGATCA TGTTCCTCCT GTATCACTAG TTTGCCAGAA 1451 TATTCCAAGA CATGTTTTAG AAGCTACCTA TGGCATTAAC ATCATAACGC 1501 CTAGAGAGGA TGAAGATCCC CACCGACCTC CAACATCGGA AGAACTGTTG 1551 ACAGCTTATG GATACATGCG AGGATTCATG ACAGCGCATG GACAGCCAGA 1601 CCAGCCTCGA TCTGCGCGCT ACATCCTGAA GGACTATGTC AGTGGTAAGC 1651 TGCTGTACTG CCATCCTCCT CCTGGAAGAG ATCCTGTAAC TTTTCAGCAT 1701 CAACACCAGC GACTCCTAGA GAACAAAATG AACAGTGATG AAATAAAAAT 1751 GCAGCTAGGC AGAAATAAAA AAGCAAAGCA GATTGAAAAT ATCGTTGACA 1801 AAACTTTTTT CCATCAAGAG AATGTGAGGG CTTTGACCAA AGGAGTCCAG 1851 GCTGTGATGG GTTACAAGCC CGGGAGTGGT GTAGTGACTG CATCCACTGC 1901 GAGCTCTGAG AACGGGGCGG GGAAGCCCTG GAAAAAACAT GGCAACAGAA 1951 ATAAAAAAGA AAAAAGTCGT AGACTCTACA AGCACCTGGA TATGTGAGGT 2001 TGGGCTGCAA CAGAAATGTC ATCTGCATTG TGCAGATGGA AAAGAGCAGA 2051 AGCTGCCTGT TGCCTGTGGA ACTGTCCCAA GACACTAGCA CTGTAGAACG 2101 GGCCCTGCTC TTGCAGAGCA CGGCTGCACC CAACAGTCTC CATGTCAAGA 2151 CCAAGGGCCT CCTGGAAACA CCAGCTCTGA CAAAAAGGAG TCATCTGGGA 2201 GCCCGAGAAT CCTACTCCTG GCCGGGCACA GTGGCTCACG CACCAACATG 2251 GAGAAACCCC GTCTCTACTA AAAATACAAA AAAATTAGCC AGGCGTGGTG 2301 GCGCGCACCT GTAATCCCAG CTACTCGGGA GGCTGAGGCA GGAGAATCAC 2351 TTGAACCAGG GAGGCAGAGT TTGCAGTGAA TGGAGATTGC GCCGCTGCAC 2401 TCCAGCCTGG GCGACAGAGT GAGACTGCAT CACAAGAAAA AAAATTTGCA 2451 AGGGATGGTT CACGAGACAC ATTTGGGACG AAGGTGAAAG AGAAATTCCC 2501 CATTCTGAGT GTCCTAGTTG GGTTCCTCCG ACTCTAAACA AGGGACTTGG 2551 GTTCAGTTAG TGTACAGCGG GGGCTCACGT CCACTAAGGA ACATGTAGAA 2601 TGTAACCACC GGGTGACAGG GAAGCTGCGG TATTTACTAC CTAGCCCCCA 2651 TCTTCACTGG TTATTCCACT TATTTAAAAT GTCCAGAATA AGCAAATCTC 2701 CATATAGAGG AAGTAGATTA GTGGTTGCTT CGGGATGGGA GGAATGGGAA 2751 GATTGAGGTC TTTCTTTTGC AGTGATAAAA ATGTCCTAAA ATTGACTGTA 2801 GCGATGGTCA CACAACTCTG AATATGCTTA AGACCATTGA ATTACACACT 2851 TTACGTTGGT GAATTGTATG GTATGTAAAT TATAGTTCAA TAACATAGTT 2901 ACAAAAGATA ATCAAAAGCA TGAAAGCACT ATTGATGTGG TTTGGATCTG 2951 TGTCCTCACC GAGTCTCATG TTGAAATGTA AGCCCCCTGG TGGGAGGCGA 3001 TGGGATTATG GGGCAGAGTC CTCACAAACG GTTTAGCACC ACCCGCTCAG 3051 TGCTGTTCTC CTGATATTGA GTCCTCATCA CATCTGGTTG CTTCAAAGTG 3101 TGTGGTGCCT CCCCTCTGTC TCCCTCCTGC TCTGGCCATA TAAGATGTGC 3151 CTGCTTCTCC TTCGCCTTCT AACATGATTG TAAGTTTCCT GAGGCCTCCC 3201 TAGAAGCAAA AGCTGCTGTG CTTCCTGTAC CATCTACTGG ACCGTGAGCC 3251 AATTAAACCT CTTTTCTTTA TAAAAAAAAA AAAAAAAAGG BLAST Results No BLAST result Medline entries No Medlme entry Peptide information for frame 3 ORF from 21 bp to 1994 bp; peptide length: 658 Category: strong similarity to known protein 1 MGRRRAPAGG SLGRALMRHQ TQRSRSHRHT DΞWLHTSELN DGYDWGRLNL 51 QSVTEQSSLD DFLATAELAG TEFVAEKLNI KFVPAEARTG LLSFEEΞQRI 101 KKLHEENKQF LCIPRRPNWN QNTTPEELKQ AEKDNFLEWR RQLVRLEEEQ 151 KLILTPFERN LDFWRQLWRV IERSDIVVQI VDARNPLLFR CEDLECYVKE 201 MDANKENVIL INKADLLTAE QRSAWAMYFE KEDVKVIFWS ALAGAIPLNG 251 DSEEEANRDD RQSNTTEFGH SSFDQAEISH SESEHLPARD SPSLSENPTT 301 DEDDSEYEDC PEEEEDDWQT CSEEDGPKEE DCSQDWKESS TADSEARSRK 351 TPQKRQIHNF SHLVSKQELL ELFKELHTGR KVKDGQLTVG LVGYPNVGKS 401 STINTIMGNK KVSVSATPGH TKHFQTLYVE PGLCLCDCPG LVMPSFVSTK 451 AEMTCSGILP IDQMRDHVPP VSLVCQNIPR HVLEATYGIN IITPREDEDP 501 HRPPTSEELL TAYGYMRGFM TAHGQPDQPR SARYILKDYV SGKLLYCHPP 551 PGRDPVTFQH QHQRLLENKM NSDEIKMQLG RNKKAKQIEN IVDKTFFHQE 601 NVRALTKGVQ AVMGYKPGSG VVTASTASSE NGAGKPWKKH GNRNKKEKSR 651 RLYKHLDM BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_8e24, frame 3 SWISSPROT :YAWG_SCHPO HYPOTHETICAL GTP-BINDING PROTEIN C3F10.16C IN CHROMOSOME I., N = 3, Score = 560, P = 1.6e-lll PIR:S64106 hypothetical protein YGL099w - yeast (Saccharomyces cerevisiae), N = 2, Score = 544, P = 2.6e-105 TREMBL :CEAF3143_1 gene: "C53H9.2"; Caenorhabditis elegans cosmid C53H9., N = 1, Score = 551, P = 2.9e-53 SWISSPROT :MMRl_MOUSE POSSIBLE GTP-BINDING PROTEIN MMR1. , N = 2, Score = 311, P = 7.5e-31 >SWISSPROT:YAWG_SCHPO HYPOTHETICAL GTP-BINDING PROTEIN C3F10.16C IN CHROMOSOME I. Length = 616 HSPs: Score = 560 (84.0 bits), Expect = 1.6e-lll, Sum P(3) = 1.6e-lll Identities = 119/253 (47%), Positives = 163/253 (64%) Query: 12 LGRALMRHQTQRSRSHRHTDSWLHTSELNDGYDWGRLNLQSVTEQSSLDDFLATAELAGT 71 LGRA+ T+ R+ + H + + R L+SVT ++ LD+FL TAEL Sbjct: 12 LGRAIQSDFTKNRRNRK—GGLKHIVDSDPKAH—RAALRSVTHETDLDEFLNTAELGEV 67 Query: 72 EFVAEKLNIKFVP-AEARTGLLSFEESQRIKKLHEENKQFLCIPRRPNWNQNTTPEELKQ 130 EF+AEK N+ + E LLS EE+ R K+ E+NK L IPRRP+W+Q TT EL + Sb j ct: 68 EFIAEKQNVTVIQNPEQNPFLLSKEEAARSKQKQEKNKDRLTIPRRPHWDQTTTAVELDR 127 Query: 131 AEKDNFLEWRRQLVRLEEEQKLILTPFERNLDFWRQLWRVIERSDIVVQIVDARNPLLFR 190 E+++FL WRR L +L++ + I+TPFERNL+ WRQLWRVIERSD+VVQIVDARNPL FR Sb j ct: 128 MEREΞFLNWRRNLAQLQDVEGFIVTPFERNLEIWRQLWRVIERSDVVVQIVDARNPLFFR 187 Query: 191 CEDLECYVKEMDANKENVILINKADLLTAEQRSAWAMYFEKEDVKVIFWSALAGAIPLNG 250 LE YVKE+ +K+N +L+NKAD+LT EQR+ W+ YF + ++ +F+SA A N Sbjct: 188 SAHLEQYVKEVGPSKKNFLLVNKADMLTEEQRNYWSSYFNENNIPFLFFSARMAA-EANE 246 Query: 251 DSEEEANRDDRQSN 264 E+ + SN Sbjct: 247 RGEDLETYESTSSN 260 Score = 532 (79.8 bits), Expect = 1.6e-lll, Sum P(3) = 1.6e-lll Identities = 131/323 (40%), Positives = 192/323 (59%) Query: 340 STADSEARSRKTPQKRQIHNFSHLVΞKQELLELFKELHTGRKVKDGQ—LTVGLVGYPNV 397 ST+ +E + +H+ S + + + L +F++ + + DG+ +T GLVGYPNV Sbjct: 256 STSSNEIPESLQADENDVHS-SRIATLKVLEGIFEKFAS—TLPDGKTKMTFGLVGYPNV 312 Query: 398 GKSSTINTIMGNKKVSVSATPGHTKHFQTLYVEPGLCLCDCPGLVMPSFVΞTKAEMTCSG 457 GKSSTIN ++G+KKVSVS+TPG TKHFQT+ + + L DCPGLV PSF +T+A++ G Sbjct: 313 GKSSTINALVGSKKVSVSΞTPGKTKHFQTINLSEKVSLLDCPGLVFPSFATTQADLVLDG 372 Query: 458 ILPIDQMRDHVPPVSLVCQNIPRHVLEATYGINI-ITPREDEDPHRPPTSEELLTAYGYM 516 +LPIDQ+R++ P +L+ + IP+ VLE Y I I I P E E P+++E+L + Sbjct: 373 VLPIDQLREYTGPSALMAERIPKEVLETLYTIRIRIKPIE-EGGTGVPSAQEVLFPFARS 431 Query: 517 RGFMTAH-GQPDQPRSARYILKDYVSGKLLYCHPPPG—RDPVTFQHQHQRLLENKMNSD 573 RGFM AH G PD R+AR +LKDYV+GKLLY HPPP F +H + + + SD Sbjct: 432 RGFMRAHHGTPDDSRAARILLKDYVNGKLLYVHPPPNYPNSGSEFNKEHHQKIVSA-TSD 490 Query: 574 EIKMQLGR NKKAKQIEN-IVDKTFFHQEN—VRALTKGVQAVM-G—YKPGSGVVTA 624 I +L R + E+ +VD +F QEN VR + KG M G YK + + Sbjct: 491 SITEKLQRTAISDNTLSAESQLVDDEYF-QENPHVRPMVKGTAVAMQGPVYKGRNTMQPF 549 Query: 625 STASSENGAGK-PWKKHGNRNKKEKSRRL 652 +++ + K P G + K+R+L Sbjct: 550 QRRLNDDASPKYPMNAQGKPLSRRKARQL 578 Score = 47 (7.1 bits), Expect = 1.3e-60, Sum P(3) = 1.3e-60 Identities = 21/84 (25%), Positives = 35/84 (41%) Query: 552 GRDPVTFQHQHQRLLENKMNSDEIKMQLGRNKKAKQIENIVDKTFFHQENVRALTKGVQA 611 G D T++ + + +DE + R K +E I +K F TK Sbjct: 248 GEDLETYESTSSNEIPESLQADENDVHSSRIATLKVLEGIFEK—FASTLPDGKTKMTFG 305 Query: 612 VMGYKPGSGVVTASTAΞSENGAGK 635 ++GY P G +ST ++ G+ K Sbjct: 306 LVGY-PNVG—KSSTINALVGSKK 326 Score = 43 (6.5 bits). Expect = 1.6e-lll, Sum P(3) = 1.6e-lll Identities = 7/13 (53%), Positives = 9/13 (69%) Query: 638 KKHGNRNKKEKSR 650 KKH +NK+ K R Sbjct: 596 KKHNKKNKRSKQR 608 Pedant information for DKFZphtes3_8e24, frame 3 Report for DKFZphtes3_8e24.3 [LENGTH] 658 [MW] 75226.58 [pi] 5.86 [HOMOL] SWISSPROT :YAWG_SCHPO HYPOTHETICAL GTP-BINDING PROTEIN C3F10.16C IN CHROMOSOME I. 5e-56 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YGL099w] 3e-55 [FUNCAT] r general function prediction [M. jannaschii, MJ1464] le-16 [FUNCAT] 08.16 extracellular transport [S. cerevisiae, YER006w] 3e-09 [PIRKW] P-loop le-27 [PIRKW] GTP binding le-27 [SUPFAM] conserved hypothetical protein MG442 7e-08 [PROSITE] ATP_GTP_A 1 [PROSITE] MYRISTYL 3 [PROSITE] AMIDATION 2 [PROSITE] CAMP_PHOSPHO_SITE 1 [PROSITE] CK2_PHOSPHO_SITE 19 [PROSITE] TYR_PHOSPHO_SITE 2 [PROSITE] PKC_PHOSPHO_SITE 10 [PROSITE] ASN_GLYCOSYLATION 2 [KW] Alpha_Beta [KW] LOW COMPLEXITY 4.56 % SEQ MGRRRAPAGGSLGRALMRHQTQRSRSHRHTDSWLHTSELNDGYDWGRLNLQSVTEQSSLD SEG xxxxxxxxxxxxx PRD cccccccccccchhhhhhhhhhhccccccccccccccccccccccchhhhhhhhccccch SEQ DFLATAELAGTEFVAEKLNIKFVPAEARTGLLSFEESQRIKKLHEENKQFLCIPRRPNWN SEG PRD hhhhhhhhhhheeeecccceeeeeeccccccchhhhhhhhhhhhhhhhhhhccccccccc SEQ QNTTPEELKQAEKDNFLEWRRQLVRLEEEQKLILTPFERNLDFWRQLWRVIERSDIVVQI SEG PRD cccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhccchhhhhhhhhhhhhhhcceeeee SEQ VDARNPLLFRCEDLECYVKEMDANKENVILINKADLLTAEQRSAWAMYFEKEDVKVIFWS SEG PRD eccccccccchhhhhhhhhhhccccceeeeecccchhhhhhhhhhhhhhhhccceeeeec SEQ ALAGAIPLNGDSEEEANRDDRQSNTTEFGHSSFDQAEISHSESEHLPARDSPSLSENPTT SEG PRD cccccccccccchhhhhhhhhhcccccccccccccccccccccccccccccccccccccc SEQ DEDDSEYEDCPEEEEDDWQTCSEEDGPKEEDCSQDWKESSTADSEARΞRKTPQKRQIHNF SEG xxxxxxxxxxxxxxxxx PRD cccccccccccccccccccccccccccccccccccccccchhhhhhhhhccccccccccc SEQ SHLVSKQELLELFKELHTGRKVKDGQLTVGLVGYPNVGKSSTINTIMGNKKVSVSATPGH SEG PRD ccccchhhhhhhhhhhhhhhccccceeeeeecccccccccceeeeccccceeeeeccccc SEQ TKHFQTLYVEPGLCLCDCPGLVMPSFVSTKAEMTCSGILPIDQMRDHVPPVSLVCQNIPR SEG PRD cceeeeeeeccceeecccccccccccchhhhhhhhccccccccccccccceeeeecccch SEQ HVLEATYGINIITPREDEDPHRPPTSEELLTAYGYMRGFMTAHGQPDQPRSARYILKDYV SEG PRD hhhhhhhhccccccccccccccccchhhhhhhhhhhhhhcccccccccchhhhhhhhhcc SEQ SGKLLYCHPPPGRDPVTFQHQHQRLLENKMNSDEIKMQLGRNKKAKQIENIVDKTFFHQE SEG PRD ccceeeeccccccccccchhhhhhhhhhcccchhhhhhhhcchhhhhhhhhhhhccccch SEQ NVRALTKGVQAVMGYKPGSGVVTASTASSENGAGKPWKKHGNRNKKEKSRRLYKHLDM SEG PRD hhhhhhhceeeeeecccccceeecccccccccccccccccccccchhhhhhhhhhccc Prosite for DKFZphtes3_8e24.3 PS00001 264->268 ASN_GLYCOSYLATION PDOC00001 PS00001 359->363 ASN_GLYCOSYLATION PDOC00001 PS00004 410->414 CAMP_PHOSPHO_SITE PDOC00004 PS00005 21->24 PKC_PHOSPHO_SITE PDOC00005 PS00005 26->29 PKC_PHOSPHO_SITE PDOC00005 PS00005 97->100 PKC_PHOSPHO_ΞITE PDOC00005 PS00005 348->351 PKC_PHOSPHO_SITE PDOC00005 PS00005 378->381 PKC_PHOΞPHO_SITE PDOC00005 PS00005 448->451 PKC_PHOSPHO_SITE PDOC00005 PS00005 493->496 PKC_PHOSPHO_SITE PDOC00005 PS00005 531->534 PKC_PHOSPHO_SITE PDOC00005 PS00005 541->544 PKC_PHOSPHO_SITE PDOC00005 PS00005 649->652 PKC_PHOSPHO_SITE PDOC00005 PS00006 52->56 CK2_PHOSPHO_ΞITE PDOC00006 PS00006 57->61 CK2_PHOSPHO_SITE PDOC00006 PS00006 93->97 CK2_PHOSPHO_SITE PDOC00006 PS00006 123->127 CK2_PHOSPHO_SITE PDOC00006 PS00006 155->159 CK2_PHOSPHO_SITE PDOC00006 PS00006 252->256 CK2_PHOSPHO_SITE PDOC00006 PS00006 271->275 CK2_PHOSPHO_SITE PDOC00006 PS00006 279->283 CK2 PHOSPHO SITE PDOC00006 so Os 0 0 σ α σ o σ σ σ σ σ o σ σ o σ o o 0 O O O O 0000000000000 0 0000000000000 DKFZphtes3_8gll group: testes derived DKFZphtes3_8gll encodes a novel proline-rich 939 amino acid protein without similarity to known proteins. The novel protein contains an ATP/GTP-bindmg site motif A (P-loop) . No informative BLAST results; No predictive prosite, pfam or - SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . unknown, prolin ritch protein 1 EST hit (from testis library) Sequenced by MediGenomix Locus : unknown Insert length: 3100 bp Poly A stretch at pos. 3056, polyadenylation signal at pos. 3041 1 AGAGTCTTCC CTCAGCATAT TTTACGATAG AGAAGATCTT GTTCCAATGG 51 AAGAAAGTGA GGACTCACAG AGTGATTCCC AGACAAGGAT TTCTGAGTCC 101 CAACACTCCC TCAAGCCAAA TTATCTTTCC CAGGCCAAGA CTGACTTCTC 151 AGAACAGTTC CAGTTGCTAG AAGATCTGCA GCTAAAAATA GCAGCAAAAC 201 TCTTAAGGAG TCAAATACCC CCCGATGTGC CTCCACCTCT AGCTTCAGGT 251 CTAGTCCTAA AATACCCTAT CTGCCTACAG TGTGGCCGAT GTTCAGGACT 301 TAATTGCCAT CATAAATTAC AGACCACTTC GGGGCCTTAT CTTCTTATCT 351 ATCCACAGCT CCACCTTGTA CGCACTCCTG AAGGCCATGG TGAGGTTCGG 401 TTGCATCTTG GCTTTAGGCT GAGAATTGGG AAAAGATCCC AAATCTCAAA 451 GTATCGTGAA AGAGATAGAC CCGTCATACG GAGAAGCCCT ATATCACCAT 501 CACAAAGGAA AGCTAAAATC TATACTCAAG CTTCCAAGAG TCCTACTTCC 551 ACAATAGATT TGCAGTCTGG GCCTTCCCAG TCCCCTGCTC CTGTACAAGT 601 CTACATCAGG CGAGGACAAC GCAGCAGGCC TGACTTAGTA GAAAAGACAA 651 AAACTAGAGC ACCTGGGCAC TATGAATTCA CTCAAGTTCA CAACCTACCA 701 GAGAGTGACT CTGAAAGCAC TCAGAATGAA AAACGGGCTA AAGTGAGAAC 751 CAAAAAGACC TCTGATTCAA AATATCCAAT GAAGAGAATC ACCAAGCGAC 801 TTAGAAAACA CAGAAAGTTC TACACAAACA GTAGAACCAC AATAGAGAGT 851 CCTTCTAGGG AATTAGCAGC CCATTTAAGA AGGAAGAGGA TTGGAGCAAC 901 TCAGACAAGT ACTGCCTCTT TAAAAAGACA ACCTAAGAAA CCTTCCCAAC 951 CCAAGTTCAT GCAACTGCTT TTTCAGAGCC TAAAGCGGGC ATTCCAAACA 1001 GCACACAGAG TTATAGCTTC TGTTGGGCGG AAGCCTGTGG ACGGGACAAG 1051 GCCAGACAAT TTGTGGGCAA GCAAAAACTA TTATCCAAAA CAAAATGCCA 1101 GGGACTATTG CTTACCAAGC AGTATCAAAA GAGACAAGAG GTCAGCTGAC 1151 AAGCTAACGC CAGCAGGCTC AACCATTAAG CAGGAGGACA TATTGTGGGG 1201 AGGAACGGTC CAGTGCAGAT CAGCTCAACA GCCAAGAAGA GCTTACTCTT 1251 TCCAACCCAG ACCTCTTCGA CTGCCCAAGC CCACAGATTC CCAAAGTGGT 1301 ATTGCTTTCC AAACTGCCTC AGTGGGGCAG CCTCTGAGAA CTGTTCAAAA 1351 GGACAGTAGT AGCAGATCAA AGAAAAACTT CTATAGAAAT GAAACCTCCA 1401 GCCAGGAGTC TAAGAACTTG TCCACACCAG GAACCAGAGT TCAGGCCCGA 1451 GGAAGAATCC TACCTGGTTC CCCTGTGAAG AGAACCTGGC ACCGACATCT 1501 TAAAGACAAA CTCACACACA AGGAGCATAA CCACCCCAGC TTCTATAGGG 1551 AGAGAACCCC ACGCGGTCCT TCTGAGAGAA CCCGTCATAA CCCCTCTTGG 1601 AGAAACCATC GCAGTCCCTC TGAGAGAAGC CAACGCAGTT CCTTGGAGAG 1651 AAGACATCAC AGTCCCTCTC AGAGGAGCCA CTGCAGTCCC TCTAGGAAAA 1701 ACCATTCCAG TCCTTCTGAG AGAAGCTGGC GCAGTCCGTC TCAGAGAAAT 1751 CACTGCAGTC CCCCCGAGAG GAGCTGTCAC AGTCTCTCTG AAAGGGGCCT 1801 TCACAGTCCC TCTCAGAGGA GCCATCGCGG TCCCTCTCAG AGAAGACATC 1851 ACAGTCCCTC AGAGAGAAGC CATCGCAGTC CCTCAGAGAG AAGCCATCGC 1901 AGTCCCTCTG AGAGAAGACA TCGCAGTCCC TCCCAGAGGA GCCATCGCGG 1951 TCCCTCAGAG AGAAGCCATT GCAGTCCCTC TGAGAGAAGA CATCGCAGTC 2001 CCTCTCAGAG GAGCCATCGT GGTCCCTCTG AGAGAAGACA TCACAGTCCC 2051 TCTAAGAGAA GCCATCGCAG TCCCGCTCGG AGGAGCCATC GCAGTCCCTC 2101 AGAGAGAAGC CATCACAGTC CCTCTGAGAG AAGCCATCAC AGTCCCTCTG 2151 AGAGAAGACA TCACAGTCCC TCTGAGAGAA GCCATTGCAG TCCCTCTGAG 2201 AGAAGCCATT GCAGTCCCTC TGAGAGAAGA CATCGCAGTC CCTCTGAGAG 2251 AAGACATCAC AGTCCCTCAG AGAAAAGCCA TCACAGTCCC TCTGAGAGAA 2301 GCCATCACAG TCCCTCTGAG AGAAGACGTC ACAGTCCCTT GGAGAGGAGC 2351 CGTCACAGTC TCTTGGAGAG GAGCCATCGC AGTCCCTCTG AGAGGAGATC 2401 TCACAGGTCC TTTGAGAGGA GCCATCGTAG GATTTCTGAG AGAAGTCACA 2451 GTCCCTCAGA GAAGAGCCAC CTCAGTCCCT TGGAAAGAAG CCGTTGCAGT 2501 CCCTCTGAGA GGAGAGGACA CAGTTCCTCT GGGAAAACCT GTCACAGTCC 2551 CTCTGAGAGA AGCCATCGCA GTCCCTCCGG GATGAGGCAA GGGAGGACCT 2601 CTGAGAGGAG CCATCGCAGT TCCTGTGAGA GAACCCGTCA CAGTCCCTCT 2651 GAGATGAGGC CAGGGAGGCC CTCTGGGAGG AACCATTGCA GTCCCTCTGA 2701 GAGGAGCCGA CGCAGTCCCC TTAAGGAGGG ACTCAAGTAC AGTTTCCCTG 2751 GAGAGAGGCC CAGCCATAGT TTGTCTAGAG ATTTCAAGAA TCAAACAACT 2801 CTCCTCGGGA CCACACATAA AAATCCCAAA GCAGGGCAAG TGTGGAGGCC 2851 TGAAGCTACT CGATGAGGCG AGGTCCGCCC CTATTATTCA TTGTCCTAAG 2901 TCTTCATCGT GCTGCCCTTT CCAGGCTTCT TTCCTGCTCA GCCACTGCCT 2951 CCAATTCCTG CGCCCCCAGC GTGGAAAGGC TTCCATTTCT CTCTACCGGG 3001 GGGGAGGCGG GTGAGAATGG GTCTGTAATT TCTCTAAGAT GAATAAAGGG 3051 GCAGTTAATT AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAGG BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 2 ORF from 47 bp to 2863 bp; peptide length: 939 Category: similarity to unknown protein Classification: unclassified Prosite motifs: ATP GTP A (824-832) 1 MEESEDSQSD SQTRISESQH SLKPNYLSQA KTDFSEQFQL LEDLQLKIAA 51 KLLRSQIPPD VPPPLASGLV LKYPICLQCG RCSGLNCHHK LQTTSGPYLL 101 IYPQLHLVRT PEGHGEVRLH LGFRLRIGKR SQISKYRERD RPVIRRSPIS 151 PSQRKAKIYT QASKSPTSTI DLQSGPSQSP APVQVYIRRG QRSRPDLVEK 201 TKTRAPGHYE FTQVHNLPES DSESTQNEKR AKVRTKKTSD SKYPMKRITK 251 RLRKHRKFYT NSRTTIESPS RELAAHLRRK RIGATQTSTA SLKRQPKKPS 301 QPKFMQLLFQ SLKRAFQTAH RVIASVGRKP VDGTRPDNLW ASKNYYPKQN 351 ARDYCLPSSI KRDKRSADKL TPAGSTIKQE DILWGGTVQC RSAQQPRRAY 401 SFQPRPLRLP KPTDSQSGIA FQTASVGQPL RTVQKDSSSR SKKNFYRNET 451 SSQESKNLST PGTRVQARGR ILPGSPVKRT WHRHLKDKLT HKEHNHPSFY 501 RERTPRGPSE RTRHNPSWRN HRSPSERSQR SSLERRHHSP SQRSHCSPSR 551 KNHSSPSERS WRSPSQRNHC SPPERSCHSL SERGLHSPSQ RSHRGPSQRR 601 HHSPSERSHR SPSERSHRSP SERRHRSPSQ RSHRGPSERS HCSPSERRHR 651 SPSQRSHRGP SERRHHSPSK RSHRSPARRS HRSPSERSHH SPSERSHHSP 701 SERRHHSPSE RSHCSPSERS HCSPSERRHR SPSERRHHSP SEKSHHSPSE 751 RSHHSPSERR RHSPLERSRH SLLERSHRSP SERRSHRSFE RSHRRISERS 801 HΞPSEKSHLS PLERSRCSPS ERRGHSSSGK TCHSPSERSH RSPSGMRQGR 851 TSERSHRSSC ERTRHSPSEM RPGRPSGRNH CSPSERSRRS PLKEGLKYSF 901 PGERPSHSLS RDFKNQTTLL GTTHKNPKAG QVWRPEATR BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_8gll, frame 2 TREMBL:AF061185_1 gene: "car90"; product: "cyst germination specific acidic repeat protein precursor"; Phytophthora infestans cyst germination specific acidic repeat protein precursor (car90) gene, complete eds., N = 1, Score = 457, P = 2.3e-39 TREMBL:AC004561_38 gene: "F16P2.41"; product: "putative proline-rich protein"; Arabidopsis thaliana chromosome II BAC F16P2 genomic sequence, complete sequence., N = 1, Score = 340, P = 4.2e-27 TREMBL:AF062655_1 product: "plenty-of-prolιnes-101"; Mus musculus plenty-of-prolιnes-101 mRNA, complete eds., N = 1, Score = 313, P = 3.6e-24 PIR:PN0099 son3 protein - human (fragment), N = 1, Score = 292, P = 1.2e-22 >TREMBL:AF061185_1 gene: "car90"; product: "cyst germination specific acidic repeat protein precursor"; Phytophthora infestans cyst germination specific acidic repeat protein precursor (car90) gene, complete eds. Length = 1,489 HSPs: Score = 457 (68.6 bits), Expect = 2.3e-39, P = 2.3e-39 Identities = 91/444 (20%), Positives = 239/444 (53%) Query: 475 SPVKRTWHRHLKDKLTHKEHNHPSFY-RERTPRGPSERTRHNPSWRNHRSPSERSQRSSL 533 +P + T + +++ T+ ++ E TP P+E T + P+ +P+E + +S Sbjct: 584 APTEETMYAPIEET-TYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYAST 642 Query: 534 ERRHHSPSQRSHCSPSRKNHSSPSERSWRSPSQRNHCSPPERSCHSLSERGLHSPSQRSH 593 E ++P++ + +P+ + P+E + +P++ +P E + ++ +E ++P++ + Sbjct: 643 EETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYAPTEETTYAPTEETT 702 Query: 594 RGPSQRRHHSPSERSHRSPSERSHRSPSERRHRSPSQRSHRGPSERSHCSPSERRHRSPS 653 P++ + P+E + +P+E + +P+E +P + + GP+E + +P+E +P+ Sbjct: 703 YAPAEETPYEPTEETTYAPTEETTYAPTEETMYAPIEETTYGPTEETTYAPTEATTYAPT 762 Query: 654 QRSHRGPSERRHHSPSKRSHRSPARRSHRSPSERSHHSPSERSHHSPSERRHHSPSERSH 713 + + P+E + P+ + +P + +P+E + ++P+E + ++P+E + P+E + Sbjct: 763 EETPYAPTEETTYEPTGETTYAPTEETTYAPTEETTYAPTEETTYAPTEETPYEPTEETT 822 Query: 714 CSPSERSHCSPSERRHRSPSERRHHSPSEKSHHSPSERSHHSPSERRRHSPLERSRHSLL 773 +P+E + P+E +P+E ++P+E++ ++P+E++ ++P+E ++P E + + Sbjct: 823 YAPTEETPYEPTEETTYTPTEETTYAPTEETTYAPTEKTTYAPTEETTYAPTEETPYEPT 882 Query: 774 ERSHRSPSERRSHRSFERS-HRRISERSHSPSEKSHLSPLERSRCSPSERRGHSSSGKTC 832 E + +P++ ++ E + + E +++P+E++ +P E + P+E ++ + +T Sbjct: 883 EETTYAPTKETTYAPTEETTYASTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETT 942 Query: 833 HSPSERSHRSPSGMRQGRTSERSHRSSCERTRHSPSEMRPGRPSGRNHCSPSERΞRRSPL 892 ++P+E + +P+ +E + + E T + P+E P+ +P+E + +P+ Sbjct: 943 YAPTEETTYAPTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETMYAPI 1002 Query: 893 KEGLKYSFPGERPSHSLSRDFKNQTT 918 +E Y+ P E +++ + + + T Sbjct: 1003 EE-TTYA-PTEETTYAPAEETPYEPT 1026 Score = 445 (66.8 bits), Expect = 4.5e-38, P = 4.5e-38 Identities = 83/394 (21%), Positives = 212/394 (53%) Query: 502 ERTPRGPSERTRHNPSWRNHRSPSERSQRSSLERRHHSPSQRSHCSPSRKNHSSPSERSW 561 E TP P+E T + P+ +P+E + + E ++P++ + +P+ + P+E + Sbjct: 763 EETPYAPTEETTYEPTGETTYAPTEETTYAPTEETTYAPTEETTYAPTEETPYEPTEETT 822 Query: 562 RSPSQRNHCSPPERSCHSLSERGLHSPSQRSHRGPSQRRHHSPSERSHRSPSERSHRSPS 621 +P++ P E + ++ +E ++P++ + P+++ ++P+E + +P+E + P+ Sbjct: 823 YAPTEETPYEPTEETTYTPTEETTYAPTEETTYAPTEKTTYAPTEETTYAPTEETPYEPT 882 Query: 622 ERRHRΞPSQRSHRGPSERSHCSPSERRHRSPSQRSHRGPSERRHHSPSKRSHRSPARRSH 681 E +P++ + P+E + + +E +P++ + P+E + P++ + +P + Sbjct: 883 EETTYAPTKETTYAPTEETTYASTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETT 942 Query: 682 RSPSERSHHSPSERSHHSPSERRHHSPSERSHCSPSERSHCSPSERRHRSPSERRHHSPS 741 +P+E + ++P+E + ++P+E ++P+E + P+E + +P+E +P+E ++P Sbjct: 943 YAPTEETTYAPTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETMYAPI 1002 Query: 742 EKSHHSPSERSHHSPSERRRHSPLERSRHSLLERSHRSPSERRSHRSFERS-HRRISERS 800 E++ ++P+E + ++P+E + P E + ++ E + +P+E ++ S E + + E + Sbjct: 1003 EETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYASTEETTYAPTEETT 1062 Query: 801 HSPSEKSHLSPLERSRCSPSERRGHSSSGKTCHSPSERSHRSPSGMRQGRTSERSHRSSC 860 ++P+E++ P E + +P+E ++ + +T ++P+E + +P+ +E + Sbjct: 1063 YAPAEETPYEPTEETTYAPTEETTYAPTEETTYAPTEETTYAPTEETTYAPAEETPYEPT 1122 Query: 861 ERTRHSPSEMRPGRPSGRNHCSPSERSRRSPLKE 894 E T ++P+E P+ +P E + P +E Sbjct: 1123 EETTYAPTEETTYAPTEETMYAPIEETTYGPTEE 1156 Score = 439 (65.9 bits), Expect = 2.0e-37, P = 2.0e-37 Identities = 86/421 (20%), Positives = 223/421 (52%) Query: 475 SPVKRTWHRHLKDKLTHKEHNHPSFY-RERTPRGPΞERTRHNPSWRNHRSPSERSQRSSL 533 +P + T + +K T+ ++ E TP P+E T + P+ +P+E + +S Sbjct: 848 APTEETTYAPT-EKTTYAPTEETTYAPTEETPYEPTEETTYAPTKETTYAPTEETTYAST 906 Query: 534 ERRHHSPSQRSHCSPSRKNHSSPSERSWRSPSQRNHCSPPERSCHΞLSERGLHSPSQRSH 593 E ++P++ + +P+ + P+E + +P++ +P E + ++ +E ++P++ + Sbjct: 907 EETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYAPTEETTYAPTEETT 966 Query: 594 RGPSQRRHHSPSERSHRSPSERSHRSPSERRHRSPSQRSHRGPSERSHCSPSERRHRSPS 653 P++ + P+E + +P+E + +P+E +P + + P+E + +P+E P+ Sbjct: 967 YAPAEETPYEPTEETTYAPTEETTYAPTEETMYAPIEETTYAPTEETTYAPAEETPYEPT 1026 Query: 654 QRSHRGPSERRHHSPSKRSHRSPARRSHRSPSERSHHSPSERSHHSPSERRHHSPSERSH 713 + + P+E ++P++ + + + +P+E + ++P+E + + P+E ++P+E + Sbjct: 1027 EETTYAPTEETTYAPTEETTYASTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETT 1086 Query: 714 CSPSERSHCSPSERRHRΞPSERRHHSPSEKSHHSPSERΞHHSPSERRRHSPLERSRHSLL 773 +P+E + +P+E +P+E ++P+E++ + P+E + ++P+E ++P E + ++ + Sbjct: 1087 YAPTEETTYAPTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETMYAPI 1146 Query: 774 ERSHRSPSERRSHRSFERS-HRRISERSHSPSEKSHLSPLERSRCSPSERRGHSSSGKTC 832 E + P+E ++ E + + E ++P+E++ P + +P+E ++ + +T Sbjct: 1147 EETTYGPTEETTYAPTEATTYAPTEETPYAPTEETTYEPTGETTYAPTEETTYAPTEETT 1206 Query: 833 HSPSERSHRSPSGMRQGRTSERSHRSSCERTRHSPSEMRPGRPSGRNHCSPSERSRRSPL 892 ++P+E + +P+ +E + + E T + P+E P+ +P+E + +P Sbjct: 1207 YAPTEETTYAPTEETPYEPTEETTYAPTEETTYEPTEETTYAPTEETTYAPTEETTYAPT 1266 Query: 893 KE 894 +E Sbjct: 1267 EE 1268 Score = 439 (65.9 bits), Expect = 2.0e-37, P = 2.0e-37 Identities = 91/434 (20%), Positives = 232/434 (53%) Query: 475 SPVKRTWHRHLKDKLTHKEHNHPSFY-RERTPRGPSERTRHNPSWRNHRSPSERSQRSSL 533 +P + T + +K T+ ++ E TP P+E T + P+ +P+E + +S Sbjct: 440 APTEETTYAPT-EKTTYAPTEETTYAPTEETPYEPTEETTYAPTKETTYAPTEETTYAST 498 Query: 534 ERRHHSPSQRSHCΞPSRKNHSSPSERSWRSPSQRNHCSPPERSCHSLΞERGLHSPSQRSH 593 E ++P++ + +P+ + P+E + +P++ +P E + ++ +E ++P++ + Sbjct: 499 EETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYAPTEETTYAPTEETT 558 Query: 594 RGPSQRRHHSPSERSHRSPSERSHRSPSERRHRSPSQRSHRGPSERSHCSPSERRHRSPS 653 P++ + P+E + +P+E + +P+E +P + + P+E + +P+E P+ Sbjct: 559 YAPAEETPYEPTEETTYAPTEETTYAPTEETMYAPIEETTYAPTEETTYAPAEETPYEPT 618 Query: 654 QRSHRGPSERRHHSPSKRSHRSPARRSHRSPSERSHHSPSERSHHSPSERRHHSPSERSH 713 + + P+E ++P++ + + + +P+E + ++P+E + + P+E ++P+E + Sbjct: 619 EETTYAPTEETTYAPTEETTYASTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETT 678 Query: 714 CSPSERSHCΞPSERRHRSPSERRHHSPSEKSHHSPSERSHHSPSERRRHΞPLERSRHSLL 773 +P+E + +P+E +P+E ++P+E++ + P+E + ++P+E ++P E + ++ + Sbjct: 679 YAPTEETTYAPTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETMYAPI 738 Query: 774 ERSHRSPSERRSHRΞFERS-HRRISERSHSPSEKSHLSPLERSRCSPSERRGHSSSGKTC 832 E + P+E ++ E + + E ++P+E++ P + +P+E ++ + +T Sbjct: 739 EETTYGPTEETTYAPTEATTYAPTEETPYAPTEETTYEPTGETTYAPTEETTYAPTEETT 798 Query: 833 HSPSERSHRSPSGMRQGRTSERΞHRSSCERTRHSPSEMRPGRPSGRNHCSPSERSRRSPL 892 ++P+E + +P T E + + E T ++P+E P P+ +P+E + +P Sbjct: 799 YAPTEETTYAP TEETPYEPT-EETTYAPTEETPYEPTEETTYTPTEETTYAPT 850 Query: 893 KEGLKYSFPGERPSHS 908 +E Y+ P E+ +++ Sbjct: 851 EE-TTYA-PTEKTTYA 864 Score = 437 (65.6 bits), Expect = 3.3e-37, P = 3.3e-37 Identities = 85/417 (20%), Positives = 223/417 (53%) Query: 502 ERTPRGPSERTRHNPSWRNHRSPSERSQRSSLERRHHSPSQRSHCSPSRKNHSSPSERSW 561 E TP P+E T + P+ +P+E + + E+ ++P++ + +P+ + P+E + Sbjct: 419 EETPYEPTEETTYTPTEETTYAPTEETTYAPTEKTTYAPTEETTYAPTEETPYEPTEETT 478 Query: 562 RSPSQRNHCSPPERSCHSLSERGLHSPSQRΞHRGPSQRRHHSPSERSHRSPSERSHRSPS 621 +P++ +P E + ++ +E ++P++ + P++ + P+E + +P+E + +P+ Sbjct: 479 YAPTKETTYAPTEETTYASTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPT 538 Query: 622 ERRHRSPSQRSHRGPSERSHCSPSERRHRSPSQRSHRGPSERRHHSPSKRSHRSPARRSH 681 E +P++ + P+E + +P+E P++ + P+E ++P++ + +P + Sbjct: 539 EETTYAPTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETMYAPIEETT 598 Query: 682 RSPSERSHHSPSERSHHSPSERRHHSPSERSHCSPSERSHCSPSERRHRSPSERRHHSPS 741 +P+E + ++P+E + + P+E ++P+E + +P+E + + +E +P+E ++P+ Sbjct: 599 YAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYASTEETTYAPTEETTYAPA 658 Query: 742 EKSHHSPSERSHHSPSERRRHSPLERSRHSLLERSHRSPSERRSHRSFERS-HRRISERS 800 E++ + P+E + ++P+E ++P E + ++ E + +P+E ++ E + + E + Sb j ct: 659 EETPYEPTEETTYAPTEETTYAPTEETTYAPTEETTYAPTEETTYAPAEETPYEPTEETT 718 Query: 801 HSPSEKSHLSPLERSRCSPSERRGHSSSGKTCHSPSERSHRSPSGMRQGRTSERSHRSSC 860 ++P+E++ +P E + +P E + + +T ++P+E + +P+ +E + Sbjct: 719 YAPTEETTYAPTEETMYAPIEETTYGPTEETTYAPTEATTYAPTEETPYAPTEETTYEPT 778 Query: 861 ERTRHSPΞEMRPGRPSGRNHCSPSERSRRSPLKEGLKYSFPGERPSHSLSRDFKNQTT 918 T ++P+E P+ +P+E + +P +E Y P E +++ + + + T Sbjct: 779 GETTYAPTEETTYAPTEETTYAPTEETTYAPTEE-TPYE-PTEETTYAPTEETPYEPT 834 Score = 428 (64.2 bits), Expect = 3. le-36, P = 3. le-36 Identities = 89/440 (20%), Positives = 228/440 (51%) Query: 473 PGSPVKRTWHRHLKDKLTHKEHNHPSFYR-ERTPRGPSERTRHNPSWRNHRSPSERSQRS 531 P P + T + K+ T+ ++ E T P+E T + P+ P+E + + Sbjct: 470 PYEPTEETTYAPTKET-TYAPTEETTYASTEETTYAPTEETTYAPAEETPYEPTEETTYA 528 Query: 532 SLERRHHSPSQRSHCSPSRKNHSSPSERSWRSPSQRNHCSPPERSCHSLSERGLHSPSQR 591 E ++P++ + +P+ + +P+E + +P++ P E + ++ +E ++P++ Sbjct: 529 PTEETTYAPTEETTYAPTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEE 588 Query: 592 SHRGPSQRRHHSPSERSHRSPSERSHRSPSERRHRSPSQRSHRGPSERΞHCSPSERRHRS 651 + P + ++P+E + +P+E + P+E +P++ + P+E + + +E + Sbjct: 589 TMYAPIEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYASTEETTYA 648 Query: 652 PSQRSHRGPSERRHHΞPSKRSHRSPARRSHRSPSERSHHSPSERSHHSPSERRHHSPSER 711 P++ + P+E + P++ + +P + +P+E + ++P+E + ++P+E ++P+E Sbjct: 649 PTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYAPTEETTYAPTEETTYAPAEE 708 Query: 712 SHCSPSERSHCSPSERRHRSPSERRHHSPSEKSHHSPSERSHHSPΞERRRHSPLERSRHS 771 + P+E + +P+E +P+E ++P E++ + P+E + ++P+E ++P E + ++ Sbjct: 709 TPYEPTEETTYAPTEETTYAPTEETMYAPIEETTYGPTEETTYAPTEATTYAPTEETPYA 768 Query: 772 LLERSHRSPSERRΞHRSFERS-HRRISERSHSPSEKSHLSPLERSRCSPSERRGHSSSGK 830 E + P+ ++ E + + E +++P+E++ +P E + P+E ++ + + Sbjct: 769 PTEETTYEPTGETTYAPTEETTYAPTEETTYAPTEETTYAPTEETPYEPTEETTYAPTEE 828 Query: 831 TCHSPSERSHRSPSGMRQGRTSERSHRSSCERTRHSPSEMRPGRPSGRNHCSPSERSRRS 890 T + P+E + +P+ +E + + E+T ++P+E P+ P+E + + Sbjct: 829 TPYEPTEETTYTPTEETTYAPTEETTYAPTEKTTYAPTEETTYAPTEETPYEPTEETTYA 888 Query: 891 PLKEGLKYSFPGERPSHSLSRD 912 P KE Y+ P E +++ + + Sbjct: 889 PTKE-TTYA-PTEETTYASTEE 908 Score = 427 (64.1 bits), Expect = 4.0e-36, P = 4.0e-36 Identities = 81/394 (20%), Positives = 213/394 (54%) Query: 502 ERTPRGPSERTRHNPSWRNHRSPSERSQRSSLERRHHSPSQRΞHCSPSRKNHSSPSERSW 561 E T GP+E T + P+ +P+E + + E + P+ + +P+ + +P+E + Sbjct: 739 EETTYGPTEETTYAPTEATTYAPTEETPYAPTEETTYEPTGETTYAPTEETTYAPTEETT 798 Query: 562 RSPSQRNHCSPPERSCHSLSERGLHΞPSQRSHRGPSQRRHHΞPSERSHRSPSERSHRSPS 621 +P++ +P E + + +E ++P++ + P++ ++P+E + +P+E + +P+ Sbjct: 799 YAPTEETTYAPTEETPYEPTEETTYAPTEETPYEPTEETTYTPTEETTYAPTEETTYAPT 858 Query: 622 ERRHRSPSQRSHRGPSERSHCSPSERRHRSPSQRSHRGPSERRHHSPSKRSHRSPARRSH 681 E+ +P++ + P+E + P+E +P++ + P+E ++ ++ + +P + Sbjct: 859 EKTTYAPTEETTYAPTEETPYEPTEETTYAPTKETTYAPTEETTYASTEETTYAPTEETT 918 Query: 682 RSPSERSHHSPSERSHHSPSERRHHSPSERSHCSPSERΞHCSPSERRHRSPSERRHHSPS 741 +P+E + + P+E + ++P+E ++P+E + +P+E + +P+E +P+E + P+ Sbjct: 919 YAPAEETPYEPTEETTYAPTEETTYAPTEETTYAPTEETTYAPTEETTYAPAEETPYEPT 978 Query: 742 EKSHHSPSERSHHSPSERRRHSPLERSRHSLLERSHRΞPSERRSHRSFERS-HRRISERS 800 E++ ++P+E + ++P+E ++P+E + ++ E + +P+E + E + + E + Sbjct: 979 EETTYAPTEETTYAPTEETMYAPIEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETT 1038 Query: 801 HSPSEKSHLSPLERΞRCSPSERRGHSSSGKTCHSPSERSHRSPSGMRQGRTSERSHRSSC 860 ++P+E++ + E + +P+E ++ + +T + P+E + +P+ +E + + Sbjct: 1039 YAPTEETTYASTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYAPT 1098 Query: 861 ERTRHSPSEMRPGRPSGRNHCSPSERSRRSPLKE 894 E T ++P+E P+ P+E + +P +E Sbjct: 1099 EETTYAPTEETTYAPAEETPYEPTEETTYAPTEE 1132 Score = 424 (63.6 bits), Expect = 8.5e-36, P = 8.5e-36 Identities = 81/394 (20%), Positives = 210/394 (53%) Query: 502 ERTPRGPSERTRHNPSWRNHRSPSERSQRSSLERRHHSPSQRSHCSPSRKNHSSPSERSW 561 E T P+E T + P+ +P+E + + E + P++ + +P+ + +P+E + Sb j ct: 939 EETTYAPTEETTYAPTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETM 998 Query: 562 RSPSQRNHCSPPERΞCHSLSERGLHSPSQRSHRGPSQRRHHSPSERSHRSPSERSHRSPS 621 +P + +P E + ++ +E + P++ + P++ ++P+E + + +E + +P+ Sbjct: 999 YAPIEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYASTEETTYAPT 1058 Query: 622 ERRHRSPSQRSHRGPSERSHCSPSERRHRSPSQRSHRGPSERRHHSPSKRSHRSPARRSH 681 E +P++ + P+E + +P+E +P++ + P+E ++P++ + +PA + Sbjct: 1059 EETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYAPTEETTYAPTEETTYAPAEETP 1118 Query: 682 RSPSERSHHSPSERSHHSPSERRHHSPSERSHCSPSERSHCSPSERRHRSPSERRHHSPS 741 P+E + ++P+E + ++P+E ++P E + P+E + +P+E +P+E ++P+ Sbjct: 1119 YEPTEETTYAPTEETTYAPTEETMYAPIEETTYGPTEETTYAPTEATTYAPTEETPYAPT 1178 Query: 742 EKSHHSPSERSHHSPSERRRHSPLERSRHSLLERSHRSPSERRSHRSFERS-HRRISERS 800 E++ + P+ + ++P+E ++P E + ++ E + +P+E + E + + E + Sbjct: 1179 EETTYEPTGETTYAPTEETTYAPTEETTYAPTEETTYAPTEETPYEPTEETTYAPTEETT 1238 Query: 801 HSPSEKSHLSPLERSRCSPSERRGHSSSGKTCHSPSERSHRSPSGMRQGRTSERSHRSSC 860 + P+E++ +P E + +P+E ++ + +T ++P + + P+ +E + + Sbjct: 1239 YEPTEETTYAPTEETTYAPTEETTYAPTEETMYAPIDETTYGPTEETTYAPTEATTYAPT 1298 Query: 861 ERTRHSPSEMRPGRPSGRNHCSPSERSRRSPLKE 894 E T ++P+E P+G +P+E + +P +E Sbjct: 1299 EETPYAPTEETTYEPTGETTYAPTEETTYAPTEE 1332 Score = 422 (63.3 bits). Expect = 1.4e-35, P = 1.4e-35 Identities = 84/407 (20%), Positives = 216/407 (53%) Query: 502 ERTPRGPSERTRHNPSWRNHRΞPSERSQRSSLERRHHSPSQRSHCSPSRKNHSSPSERSW 561 E T P+E T + P+ P+E + + E + P++ + +P+ + +P+E + Sbjct: 795 EETTYAPTEETTYAPTEETPYEPTEETTYAPTEETPYEPTEETTYTPTEETTYAPTEETT 854 Query: 562 RSPSQRNHCSPPERSCHSLSERGLHSPSQRSHRGPSQRRHHSPΞERSHRSPSERSHRSPS 621 +P+++ +P E + ++ +E + P++ + P++ ++P+E + + +E + +P+ Sbjct: 855 YAPTEKTTYAPTEETTYAPTEETPYEPTEETTYAPTKETTYAPTEETTYASTEETTYAPT 914 Query: 622 ERRHRSPSQRSHRGPSERSHCSPSERRHRSPSQRSHRGPSERRHHSPSKRSHRSPARRSH 681 E +P++ + P+E + +P+E +P++ + P+E ++P++ + +PA + Sbjct: 915 EETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYAPTEETTYAPTEETTYAPAEETP 974 Query: 682 RSPSERSHHSPSERSHHSPSERRHHSPSERSHCSPSERSHCSPSERRHRSPSERRHHSPS 741 P+E + ++P+E + ++P+E ++P E + +P+E + +P+E P+E ++P+ Sbjct: 975 YEPTEETTYAPTEETTYAPTEETMYAPIEETTYAPTEETTYAPAEETPYEPTEETTYAPT 1034 Query: 742 EKSHHSPSERSHHSPSERRRHSPLERSRHSLLERSHRSPSERRΞHRSFERS-HRRISERS 800 E++ ++P+E + ++ +E ++P E + ++ E + P+E ++ E + + E + Sbjct: 1035 EETTYAPTEETTYASTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETT 1094 Query: 801 HSPSEKSHLSPLERSRCSPSERRGHSSSGKTCHSPSERSHRSPSGMRQGRTSERSHRSSC 860 ++P+E++ +P E + +P+E + + +T ++P+E + +P+ E + Sbjct: 1095 YAPTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETMYAPIEETTYGPT 1154 Query: 861 ERTRHSPSEMRPGRPSGRNHCSPSERSRRSPLKEGLKYSFPGERPΞHS 908 E T ++P+E P+ +P+E + P E Y+ P E +++ Sbjct: 1155 EETTYAPTEATTYAPTEETPYAPTEETTYEPTGE-TTYA-PTEETTYA 1200 Score = 421 (63.2 bits), Expect = 1.8e-35, P = 1.8e-35 Identities = 86/418 (20%), Positives = 219/418 (52%) Query: 491 HKEHNHPSFYRERTPRGPSERTRHNPSWRNHRSPSERSQRSSLERRHHSPSQRSHCSPSR 550 H H E T P+E T + P+ +P+E + + E + P++ + +P+ Sbjct: 376 HYAHIEKPCDTEVTMYAPTEETTYAPTEETTYAPTEETTYAPTEETPYEPTEETTYTPTE 435 Query: 551 KNHSSPSERSWRSPSQRNHCSPPERSCHSLSERGLHSPSQRSHRGPSQRRHHSPSERSHR 610 + +P+E + +P+++ +P E + ++ +E + P++ + P++ ++P+E + Sbjct: 436 ETTYAPTEETTYAPTEKTTYAPTEETTYAPTEETPYEPTEETTYAPTKETTYAPTEETTY 495 Query: 611 SPSERSHRSPSERRHRSPSQRSHRGPSERSHCSPSERRHRSPSQRSHRGPSERRHHSPSK 670 + +E + +P+E +P++ + P+E + +P+E +P++ + P+E ++P++ Sbjct: 496 ASTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYAPTEETTYAPTE 555 Query: 671 RSHRSPARRSHRSPSERSHHSPSERSHHSPSERRHHSPSERSHCSPSERSHCSPSERRHR 730 + +PA + P+E + ++P+E + ++P+E ++P E + +P+E + +P+E Sbjct: 556 ETTYAPAEETPYEPTEETTYAPTEETTYAPTEETMYAPIEETTYAPTEETTYAPAEETPY 615 Query: 731 SPSERRHHSPSEKSHHSPSERSHHSPSERRRHSPLERSRHSLLERSHRSPSERRSHRSFE 790 P+E ++P+E++ ++P+E + ++ +E ++P E + ++ E + P+E ++ E Sbjct: 616 EPTEETTYAPTEETTYAPTEETTYASTEETTYAPTEETTYAPAEETPYEPTEETTYAPTE 675 Query: 791 RS-HRRISERSHSPSEKSHLSPLERSRCSPSERRGHSSSGKTCHSPSERSHRSPSGMRQG 849 + + E +++P+E++ +P E + +P+E + + +T ++P+E + +P+ Sbjct: 676 ETTYAPTEETTYAPTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETMY 735 Query: 850 RTSERSHRSSCERTRHSPSEMRPGRPSGRNHCSPSERSRRSPLKEGLKYSFPGERPΞHS 908 E + E T ++P+E P+ +P+E + P E Y+ P E +++ Sbjct: 736 APIEETTYGPTEETTYAPTEATTYAPTEETPYAPTEETTYEPTGE-TTYA-PTEETTYA 792 Score = 420 (63.0 bits), Expect = 2.3e-35, P = 2.3e-35 Identities = 82/393 (20%), Positives = 206/393 (52%) Query: 502 ERTPRGPSERTRHNPSWRNHRSPSERSQRSSLERRHHSPSQRSHCSPSRKNHSSPSERSW 561 E TP P+E T + P+ +P+E + + +E ++P++ + +P+ + P+E + Sbjct: 971 EETPYEPTEETTYAPTEETTYAPTEETMYAPIEETTYAPTEETTYAPAEETPYEPTEETT 1030 Query: 562 RSPSQRNHCSPPERSCHSLSERGLHSPSQRSHRGPSQRRHHSPSERSHRSPSERSHRSPS 621 +P++ +P E + ++ +E ++P++ + P++ + P+E + +P+E + +P+ Sbjct: 1031 YAPTEETTYAPTEETTYASTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPT 1090 Query: 622 ERRHRSPSQRSHRGPSERSHCSPSERRHRSPSQRSHRGPSERRHHSPSKRSHRSPARRSH 681 E +P++ + P+E + +P+E P++ + P+E ++P++ + +P + Sbjct: 1091 EETTYAPTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETMYAPIEETT 1150 Query: 682 RSPSERSHHSPSERSHHSPSERRHHSPSERSHCSPSERSHCSPSERRHRSPSERRHHSPS 741 P+E + ++P+E + ++P+E ++P+E + P+ + +P+E +P+E ++P+ Sbjct: 1151 YGPTEETTYAPTEATTYAPTEETPYAPTEETTYEPTGETTYAPTEETTYAPTEETTYAPT 1210 Query: 742 EKSHHSPSERSHHSPSERRRHSPLERSRHSLLERSHRSPSERRSHRSFERS-HRRISERS 800 E++ ++P+E + + P+E ++P E + + E + +P+E ++ E + + E Sbjct: 1211 EETTYAPTEETPYEPTEETTYAPTEETTYEPTEETTYAPTEETTYAPTEETTYAPTEETM 1270 Query: 801 HSPSEKSHLSPLERSRCSPSERRGHSSSGKTCHSPSERSHRSPΞGMRQGRTSERΞHRSSC 860 ++P +++ P E + +P+E ++ + +T ++P+E + P+G +E + + Sbjct: 1271 YAPIDETTYGPTEETTYAPTEATTYAPTEETPYAPTEETTYEPTGETTYAPTEETTYAPT 1330 Query: 861 ERTRHSPSEMRPGRP SGRNHCSPSE 885 E T ++P E P P S C+ E Sbjct: 1331 EETTYAPMEETPYEPAEESTSTVSTEKPCNTEE 1363 Score = 419 (62.9 bits), Expect = 3.0e-35, P = 3.0e-35 Identities = 83/411 (20%), Positives = 215/411 (52%) Query: 502 ERTPRGPSERTRHNPSWRNHRSPSERSQRSSLERRHHSPSQRSHCSPSRKNHSSPSERSW 561 E T P+E T + P+ +P+E + E ++P++ + +P+ + +P E + Sbjct: 947 EETTYAPTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETMYAPIEETT 1006 Query: 562 RSPSQRNHCSPPERSCHSLSERGLHSPSQRSHRGPSQRRHHSPΞERSHRSPSERSHRSPS 621 +P++ +P E + + +E ++P++ + P++ ++ +E + +P+E + +P+ Sbjct: 1007 YAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYASTEETTYAPTEETTYAPA 1066 Query: 622 ERRHRSPSQRSHRGPSERSHCSPSERRHRSPSQRSHRGPSERRHHSPSKRSHRSPARRSH 681 E P++ + P+E + +P+E +P++ + P+E ++P++ + P + Sbjct: 1067 EETPYEPTEETTYAPTEETTYAPTEETTYAPTEETTYAPTEETTYAPAEETPYEPTEETT 1126 Query: 682 RSPSERSHHSPSERSHHSPSERRHHSPSERSHCSPSERΞHCSPSERRHRSPSERRHHSPΞ 741 +P+E + ++P+E + ++P E + P+E + +P+E + +P+E +P+E + P+ Sbjct: 1127 YAPTEETTYAPTEETMYAPIEETTYGPTEETTYAPTEATTYAPTEETPYAPTEETTYEPT 1186 Query: 742 EKSHHSPSERSHHSPSERRRHSPLERSRHSLLERSHRSPSERRSHRSFERS-HRRISERS 800 ++ ++P+E + ++P+E ++P E + ++ E + P+E ++ E + + E + Sbjct: 1187 GETTYAPTEETTYAPTEETTYAPTEETTYAPTEETPYEPTEETTYAPTEETTYEPTEETT 1246 Query: 801 HSPSEKSHLSPLERSRCSPSERRGHSSSGKTCHSPSERSHRSPSGMRQGRTSERSHRSSC 860 ++P+E++ +P E + +P+E ++ +T + P+E + +P+ +E + + Sbjct: 1247 YAPTEETTYAPTEETTYAPTEETMYAPIDETTYGPTEETTYAPTEATTYAPTEETPYAPT 1306 Query: 861 ERTRHSPSEMRPGRPSGRNHCSPSERSRRSPLKEGLKYSFPGERPSHSLSRD 912 E T + P+ P+ +P+E + +P++E Y P E + ++S + Sbjct: 1307 EETTYEPTGETTYAPTEETTYAPTEETTYAPMEE-TPYE-PAEESTSTVΞTE 1356 Score = 415 (62.3 bits), Expect = 8.0e-35, P = 8.0e-35 Identities = 84/423 (19%), Positives = 218/423 (51%) Query: 473 PGSPVKRTWHRHLKDKLTHKEHNHPSFYR-ERTPRGPSERTRHNPSWRNHRSPSERSQRS 531 P P + T + K+ T+ ++ E T P+E T + P+ P+E + + Sbjct: 878 PYEPTEETTYAPTKET-TYAPTEETTYAΞTEETTYAPTEETTYAPAEETPYEPTEETTYA 936 Query: 532 SLERRHHSPSQRSHCSPSRKNHSSPSERSWRSPSQRNHCSPPERSCHSLSERGLHSPSQR 591 E ++P++ + +P+ + +P+E + +P++ P E + ++ +E ++P++ Sbjct: 937 PTEETTYAPTEETTYAPTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEE 996 Query: 592 SHRGPSQRRHHSPSERSHRSPSERSHRSPSERRHRSPSQRSHRGPSERSHCSPSERRHRS 651 + P + ++P+E + +P+E + P+E +P++ + P+E + + +E + Sbjct: 997 TMYAPIEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYASTEETTYA 1056 Query: 652 PSQRSHRGPSERRHHSPSKRSHRSPARRSHRSPSERSHHSPSERSHHSPSERRHHSPSER 711 P++ + P+E + P++ + +P + +P+E + ++P+E + ++P+E ++P+E Sbjct: 1057 PTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYAPTEETTYAPTEETTYAPAEE 1116 Query: 712 SHCSPSERSHCSPSERRHRSPSERRHHSPSEKSHHSPSERSHHSPSERRRHSPLERSRHS 771 + P+E + +P+E +P+E ++P E++ + P+E + ++P+E ++P E + ++ Sbjct: 1117 TPYEPTEETTYAPTEETTYAPTEETMYAPIEETTYGPTEETTYAPTEATTYAPTEETPYA 1176 Query: 772 LLERSHRSPSERRSHRSFERS-HRRIΞERSHSPSEKSHLSPLERSRCSPSERRGHSSSGK 830 E + P+ ++ E + + E +++P+E++ +P E + P+E ++ + + Sbjct: 1177 PTEETTYEPTGETTYAPTEETTYAPTEETTYAPTEETTYAPTEETPYEPTEETTYAPTEE 1236 Query: 831 TCHSPSERSHRSPSGMRQGRTSERSHRSSCERTRHSPSEMRPGRPΞGRNHCSPSERSRRS 890 T + P+E + +P+ +E + + E T ++P + P+ +P+E + + Sbjct: 1237 TTYEPTEETTYAPTEETTYAPTEETTYAPTEETMYAPIDETTYGPTEETTYAPTEATTYA 1296 Query: 891 PLKE 894 P +E Sbjct: 1297 PTEE 1300 Score = 403 (60.5 bits), Expect = 1.6e-33, P = 1.6e-33 Identities = 84/394 (21%), Positives = 213/394 (54%) Query: 501 RERTPRGPSERTRHNPSWRNHRSPSERΞQRSSLERRHHSPSQRSHCSPSRKNHSSPSERS 560 RE T PSE T + P +P+E+ +E + + ++ +P++ ++P+ER Sbjct: 319 REETTAAPSEDTTYAPREVTPYAPTEKPY—DVEETTYVTEESTY-APTKSETNAPTERM 375 Query: 561 WRSPSQRNHCSPPERSCHSLSERGLHSPSQRSHRGPSQRRHHSPSERSHRSPSERSHRSP 620 + ++ C E + ++ +E ++P++ + P++ ++P+E + P+E + +P Sbjct: 376 HYAHIEKP-CDT-EVTMYAPTEETTYAPTEETTYAPTEETTYAPTEETPYEPTEETTYTP 433 Query: 621 SERRHRSPSQRSHRGPSERSHCSPSERRHRSPSQRSHRGPSERRHHSPSKRSHRSPARRS 680 +E +P++ + P+E++ +P+E +P++ + P+E ++P+K + +P + Sbjct: 434 TEETTYAPTEETTYAPTEKTTYAPTEETTYAPTEETPYEPTEETTYAPTKETTYAPTEET 493 Query: 681 HRSPSERSHHSPSERSHHSPΞERRHHSPSERSHCSPSERSHCSPSERRHRSPSERRHHSP 740 + +E + ++P+E + ++P+E + P+E + +P+E + +P+E +P+E ++P Sbjct: 494 TYASTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYAPTEETTYAP 553 Query: 741 SEKSHHSPSERSHHSPSERRRHSPLERSRHSLLERSHRSPSERRSHRSFERS-HRRISER 799 +E++ ++P+E + + P+E ++P E + ++ E + +P E ++ E + + E Sbjct: 554 TEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETMYAPIEETTYAPTEETTYAPAEET 613 Query: 800 SHSPSEKSHLSPLERSRCSPSERRGHSSSGKTCHSPSERSHRSPΞGMRQGRTSERSHRSS 859 + P+E++ +P E + +P+E ++S+ +T ++P+E + +P+ +E + + Sbjct: 614 PYEPTEETTYAPTEETTYAPTEETTYASTEETTYAPTEETTYAPAEETPYEPTEETTYAP 673 Query: 860 CERTRHSPSEMRPGRPSGRNHCSPSERSRRSPLKE 894 E T ++P+E P+ +P+E + +P +E Sbjct: 674 TEETTYAPTEETTYAPTEETTYAPTEETTYAPAEE 708 Score = 398 (59.7 bits), Expect = 5.5e-33, P = 5.5e-33 Identities = 84/402 (20%), Positives = 209/402 (51%) Query: 475 SPVKRTWHRHLKDKLTHKEHNHPSFY-RERTPRGPSERTRHNPSWRNHRSPSERSQRSSL 533 +P + T + +++ T+ ++ E TP P+E T + P+ +P+E + +S Sbjct: 992 APTEETMYAPIEET-TYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYAST 1050 Query: 534 ERRHHSPSQRSHCSPSRKNHSSPSERSWRSPSQRNHCSPPERSCHSLSERGLHSPSQRSH 593 E ++P++ + +P+ + P+E + +P++ +P E + ++ +E ++P++ + Sbjct: 1051 EETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYAPTEETTYAPTEETT 1110 Query: 594 RGPSQRRHHSPSERSHRSPSERSHRSPSERRHRSPSQRSHRGPΞERSHCSPSERRHRSPS 653 P++ + P+E + +P+E + +P+E +P + + GP+E + +P+E +P+ Sbjct: llll YAPAEETPYEPTEETTYAPTEETTYAPTEETMYAPIEETTYGPTEETTYAPTEATTYAPT 1170 Query: 654 QRSHRGPSERRHHSPSKRSHRSPARRSHRSPΞERSHHSPSERSHHSPSERRHHSPSERSH 713 + + P+E + P+ + +P + +P+E + ++P+E + ++P+E + P+E + Sbjct: 1171 EETPYAPTEETTYEPTGETTYAPTEETTYAPTEETTYAPTEETTYAPTEETPYEPTEETT 1230 Query: 714 CSPSERSHCSPSERRHRSPSERRHHSPSEKSHHSPSERSHHSPSERRRHSPLERSRHSLL 773 +P+E + P+E +P+E ++P+E++ ++P+E + ++P + + P E + ++ Sbjct: 1231 YAPTEETTYEPTEETTYAPTEETTYAPTEETTYAPTEETMYAPIDETTYGPTEETTYAPT 1290 Query: 774 ERSHRSPSERRSHRSFERSHRRISERSHSPSEKΞHLSPLERSRCSPSERRGHSSSGKTCH 833 E + +P+E + E E ++ P+ ++ +P E + +P+E ++ +T + Sbjct: 1291 EATTYAPTEETPYAPTE ETTYEPTGETTYAPTEETTYAPTEETTYAPMEETPY 1343 Query: 834 SPΞERSHRSPSGMRQGRTSERSHRSSCERTRHSPSEMRPGRPS 876 P+E S + S + T E + + E T PS+ P+ Sb j ct: 1344 EPAEESTSTVSTEKPCNTEEFTDEPTDEPT-DEPSDEPTDEPT 1385 Score = 368 (55.2 bits), Expect = 9.5e-30, P = 9.5e-30 Identities = 79/386 (20%), Positives = 211/386 (54%) Query: 524 PSERSQRSSLERRHHSPSQRSHCSPSRKNHSSPSERSWRSPSQRNHCSPPERSCHSLSER 583 PS+ ++ + E + P + + +PS +P E + +P+++ + E + + ++E Sb j ct: 303 PSDETEAPT-EGTTYVPREETTAAPSEDTTYAPREVTPYAPTEKPY—DVEETTY-VTEE 358 Query: 584 GLHSPSQRSHRGPSQRRHHSPΞER SHRSPSERSHRSPSERRHRSPΞQRSHRGPS 637 ++P++ P++R H++ E+ + +P+E + +P+E +P++ + P+ Sbjct: 359 STYAPTKSETNAPTERMHYAHIEKPCDTEVTMYAPTEETTYAPTEETTYAPTEETTYAPT 418 Query: 638 ERSHCSPSERRHRSPSQRSHRGPSERRHHSPSKRSHRSPARRSHRSPSERSHHSPSERSH 697 E + P+E +P++ + P+E ++P++++ +P + +P+E + + P+E + Sbjct: 419 EETPYEPTEETTYTPTEETTYAPTEETTYAPTEKTTYAPTEETTYAPTEETPYEPTEETT 478 Query: 698 HSPSERRHHSPSERSHCSPSERSHCSPSERRHRSPSERRHHSPSEKSHHSPSERSHHSPS 757 ++P++ ++P+E + + +E + +P+E +P+E + P+E++ ++P+E + ++P+ Sbjct: 479 YAPTKETTYAPTEETTYASTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPT 538 Query: 758 ERRRHSPLERSRHSLLERSHRSPSERRSHRSFERS-HRRISERSHSPSEKSHLSPLERSR 816 E ++P E + ++ E + +P+E + E + + E +++P+E++ +P+E + Sbjct: 539 EETTYAPTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETMYAPIEETT 598 Query: 817 CSPSERRGHSSSGKTCHSPSERSHRSPSGMRQGRTSERSHRΞSCERTRHSPSEMRPGRPS 876 +P+E ++ + +T + P+E + +P+ +E + +S E T ++P+E P+ Sbjct: 599 YAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYASTEETTYAPTEETTYAPA 658 Query: 877 GRNHCSPSERSRRSPLKEGLKYSFPGERPSHS 908 P+E + +P +E Y+ P E +++ Sbjct: 659 EETPYEPTEETTYAPTEE-TTYA-PTEETTYA 688 Score = 337 (50.6 bits), Expect = 2. le-26, P = 2. le-26 Identities = 66/328 (20%), Positives = 170/328 (51%) Query: 502 ERTPRGPSERTRHNPSWRNHRSPSERSQRSSLERRHHSPSQRSHCSPSRKNHSSPSERSW 561 E T P+E T + P+ +P+E + + E ++P++ + +P+ + +P+E + Sbjct: 1059 EETTYAPAEETPYEPTEETTYAPTEETTYAPTEETTYAPTEETTYAPTEETTYAPAEETP 1118 Query: 562 RSPSQRNHCSPPERSCHSLSERGLHSPSQRSHRGPSQRRHHSPSERSHRSPSERSHRSPS 621 P++ +P E + ++ +E +++P + + GP++ ++P+E + +P+E + +P+ Sbjct: 1119 YEPTEETTYAPTEETTYAPTEETMYAPIEETTYGPTEETTYAPTEATTYAPTEETPYAPT 1178 Query: 622 ERRHRSPSQRSHRGPSERSHCSPSERRHRSPSQRSHRGPSERRHHSPSKRSHRSPARRSH 681 E P+ + P+E + +P+E +P++ + P+E + P++ + +P + Sbjct: 1179 EETTYEPTGETTYAPTEETTYAPTEETTYAPTEETTYAPTEETPYEPTEETTYAPTEETT 1238 Query: 682 RSPSERSHHSPSERSHHSPSERRHHSPSERSHCSPSERSHCSPSERRHRΞPSERRHHSPS 741 P+E + ++P+E + ++P+E ++P+E + +P + + P+E +P+E ++P+ Sbjct: 1239 YEPTEETTYAPTEETTYAPTEETTYAPTEETMYAPIDETTYGPTEETTYAPTEATTYAPT 1298 Query: 742 EKSHHSPΞERSHHSPSERRRHSPLERSRHSLLERSHRSPSERRSHRSFERSHRRIS 797 E++ ++P+E + + P+ ++P E + ++ E + +P E + E S +S Sbjct: 1299 EETPYAPTEETTYEPTGETTYAPTEETTYAPTEETTYAPMEETPYEPAEESTSTVSTEKP 1358 Query: 798 ERSHSPSEKSHLSPLERSRCSPSE 821 E + P+++ P + P++ Sbjct: 1359 CNTEEFTDEPTDEPTDEPSDEPTDEPTD 1386 Score = 333 (50.0 bits), Expect = 5.7e-26, P = 5.7e-26 Identities = 63/320 (19%), Positives = 166/320 (51%) Query: 502 ERTPRGPSERTRHNPSWRNHRSPSERSQRSSLERRHHSPSQRSHCSPSRKNHΞSPSERSW 561 E T P+E T + P+ +P+E + + E ++P++ + P+ + +P+E + Sbjct: 1075 EETTYAPTEETTYAPTEETTYAPTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETT 1134 Query: 562 RSPSQRNHCSPPERSCHSLSERGLHSPSQRSHRGPSQRRHHSPSERSHRSPSERSHRSPS 621 +P++ +P E + + +E ++P++ + P++ ++P+E + P+ + +P+ Sbjct: 1135 YAPTEETMYAPIEETTYGPTEETTYAPTEATTYAPTEETPYAPTEETTYEPTGETTYAPT 1194 Query: 622 ERRHRSPSQRSHRGPSERSHCSPSERRHRSPSQRSHRGPSERRHHSPΞKRSHRSPARRSH 681 E +P++ + P+E + +P+E P++ + P+E + P++ + +P + Sbjct: 1195 EETTYAPTEETTYAPTEETTYAPTEETPYEPTEETTYAPTEETTYEPTEETTYAPTEETT 1254 Query: 682 RSPSERSHHSPSERSHHSPSERRHHSPSERSHCSPSERSHCSPSERRHRSPSERRHHSPΞ 741 +P+E + ++P+E + ++P + + P+E + +P+E + +P+E +P+E + P+ Sbjct: 1255 YAPTEETTYAPTEETMYAPIDETTYGPTEETTYAPTEATTYAPTEETPYAPTEETTYEPT 1314 Query: 742 EKSHHSPSERSHHSPSERRRHSPLERSRHSLLERSHRSPSERRSHRSFERSHRRISERSH 801 ++ ++P+E + ++P+E ++P+E + + E S + S + + E + E + Sbjct: 1315 GETTYAPTEETTYAPTEETTYAPMEETPYEPAEESTSTVSTEKPCNTEEFTDEPTDEPTD 1374 Query: 802 SPSEKSHLSPLERSRCSPSE 821 PS++ P + P++ Sbjct: 1375 EPSDEPTDEPTDEPTDLPTD 1394 Score = 303 (45.5 bits), Expect = 9.6e-23, P = 9.6e-23 Identities = 70/322 (21%), Positives = 170/322 (52%) Query: 584 GLHSPSQRSHRGPSQRRHHSPSERSHRSPSERSHRSPSERRHRSPSQRΞHRGPSERSHCS 643 G + PS + P++ + P E + +PSE + +P E +P+++ + E ++ + Sbjct: 299 GGYEPΞDETE-APTEGTTYVPREETTAAPSEDTTYAPREVTPYAPTEKPY-DVEETTYVT 356 Query: 644 PSERRHRSPSQRSHRGPSERRHHSPSKRSHRΞPARRSHRSPSERSHHSPSERSHHSPSER 703 E +P++ P+ER H++ ++ + + +P+E + ++P+E + ++P+E Sbjct: 357 --EESTYAPTKSETNAPTERMHYAHIEKPCDTEV— MYAPTEETTYAPTEETTYAPTEE 412 Query: 704 RHHSPSERSHCSPSERSHCSPSERRHRSPSERRHHSPSEKSHHSPSERSHHSPSERRRHS 763 ++P+E + P+E + +P+E +P+E ++P+EK+ ++P+E + ++P+E + Sbjct: 413 TTYAPTEETPYEPTEETTYTPTEETTYAPTEETTYAPTEKTTYAPTEETTYAPTEETPYE 472 Query: 764 PLERSRHSLLERSHRSPSERRSHRSFERS-HRRISERSHSPSEKSHLSPLERSRCSPSER 822 P E + ++ + + +P+E ++ S E + + E +++P+E++ P E + +P+E Sbjct: 473 PTEETTYAPTKETTYAPTEETTYASTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEE 532 Query: 823 RGHSSSGKTCHSPSERSHRSPSGMRQGRTSERSHRSSCERTRHSPSEMRPGRPSGRNHCΞ 882 ++ + +T ++P+E + +P+ +E + E T ++P+E P+ + Sbjct: 533 TTYAPTEETTYAPTEETTYAPTEETTYAPAEETPYEPTEETTYAPTEETTYAPTEETMYA 592 Query: 883 PSERSRRSPLKEGLKYSFPGERP 905 P E + +P +E Y+ E P Sbjct: 593 PIEETTYAPTEE-TTYAPAEETP 614 Score = 151 (22.7 bits), Expect = 2.0e-06, P = 2.0e-06 Identities = 45/198 (22%), Positives = 103/198 (52%) Query: 716 PSERSHCSPSERRHRSPSERRHHSPSEKSHHSPSERSHHSPSERRRHSPLERSRHSLLER 775 PS+ + +P+E P E +PSE + ++P E + ++P+E+ +E + + + E Sbjct: 303 PSDETE-APTEGTTYVPREETTAAPSEDTTYAPREVTPYAPTEKPYD--VEETTY-VTEE 358 Query: 776 SHRSPSERRSHRSFERSHRRISERS HSPSEKSHLSPLERSRCSPSERRGHSSS 828 S +P++ ++ ER H E+ ++P+E++ +P E + +P+E ++ + Sbjct: 359 STYAPTKSETNAPTERMHYAHIEKPCDTEVTMYAPTEETTYAPTEETTYAPTEETTYAPT 418 Query: 829 GKTCHSPSERSHRSPSGMRQGRTSERSHRSSCERTRHSPSEMRPGRPSGRNHCSPSERSR 888 +T + P+E + +P+ +E + + E+T ++P+E P+ P+E + Sbjct: 419 EETPYEPTEETTYTPTEETTYAPTEETTYAPTEKTTYAPTEETTYAPTEETPYEPTEETT 478 Query: 889 RSPLKEGLKYSFPGERPSHSLSRD 912 +P KE Y+ P E +++ + + Sbjct: 479 YAPTKE-TTYA-PTEETTYASTEE 500 Pedant information for DKFZphtes3_8gll, frame 2 Report for DKFZphtes3_8gll .2 [LENGTH] 954 [MW] 110063.05 [pi] 11.40 [PROSITE] ATP_GTP_A 1 [KW] Irregular [KW] LOW_COMPLEXITY 27.67 % SEQ ESSLSIFYDREDLVPMEESEDSQSDSQTRISESQHSLKPNYLSQAKTDFSEQFQLLEDLQ SEG xxxxxxxxxxx PRD ccceeeccccccccccccccccccccccccccccccccccccchhhhhhhhhhhhhhhhh SEQ LKIAAKLLRSQIPPDVPPPLASGLVLKYPICLQCGRCSGLNCHHKLQTTSGPYLLIYPQL SEG PRD hhhhhhhhhhcccccccccccceeeeecceeecccccccccccccccccccceeeehhhh SEQ HLVRTPEGHGEVRLHLGFRLRIGKRSQISKYRERDRPVIRRSPISPSQRKAKIYTQASKS SEG PRD hcccccccccceeecccceeeccccccccccccccceeeeeccccccchhhhhhhccccc SEQ PTSTIDLQSGPSQSPAPVQVYIRRGQRSRPDLVEKTKTRAPGHYEFTQVHNLPESDSEST SEG PRD ccccccccccccccccceeeeeeeccccccchhhhhhcccccceeeeeecccccccccch SEQ QNEKRAKVRTKKTSDSKYPMKRITKRLRKHRKFYTNSRTTIESPSRELAAHLRRKRIGAT SEG PRD hhhhhhhhhhccccccccccchhhhhhhhhhhccccccccccccchhhhhhhhhhhhhcc SEQ QTSTASLKRQPKKPSQPKFMQLLFQSLKRAFQTAHRVIASVGRKPVDGTRPDNLWASKNY SEG PRD ccchhhhhccccccccchhhhhhhhhhhhhhhhhhhhhhccccccccccccccccccccc SEQ YPKQNARDYCLPSSIKRDKRSADKLTPAGSTIKQEDILWGGTVQCRSAQQPRRAYSFQPR SEG PRD cccccccccccccccccccccccccccccccccccceeeccccccccccccccccccccc SEQ PLRLPKPTDSQSGIAFQTASVGQPLRTVQKDSSSRSKKNFYRNETSSQESKNLΞTPGTRV SEG PRD ccccccccccccceeeecccccccceeeeeccccccccccccccccccccccccccccee SEQ QARGRILPGSPVKRTWHRHLKDKLTHKEHNHPΞFYRERTPRGPSERTRHNPSWRNHRSPS SEG xxxxx PRD eeecccccccccccccccccccccccccccccceeeeccccccccccccccccccccccc SEQ ERSQRSSLERRHHSPSQRSHCSPSRKNHSΞPSERSWRSPSQRNHCSPPERΞCHSLSERGL SEG xxxxxxxxxxxxxx xxxxxxxxxxxx PRD chhhhhhhhhhccccccccccccccccccccccccccccccccccccccccccccccccc SEQ HSPSQRSHRGPSQRRHHSPSERSHRSPSERSHRSPSERRHRSPSQRSHRGPSERSHCSPS SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ ERRHRSPSQRSHRGPSERRHHSPSKRSHRSPARRSHRSPSERSHHSPSERSHHSPSERRH SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ HSPΞERSHCSPSERSHCSPSERRHRSPSERRHHSPSEKSHHSPSERSHHSPSERRRHSPL SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ ERSRHSLLERSHRSPSERRSHRSFERSHRRISERSHSPSEKSHLSPLERSRCSPSERRGH SEG xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx PRD hhhhhhhhhhhccccccccchhhhhhhhhhhhhccccccccccccccccccccccccccc SEQ SSSGKTCHSPSERSHRSPSGMRQGRTSERSHRSSCERTRHSPSEMRPGRPSGRNHCSPSE SEG xxxxxxxxxxxx PRD cccccccccccccccccccccccccccccccccccccccccccccccccccccccccccc SEQ RSRRSPLKEGLKYSFPGERPSHSLSRDFKNQTTLLGTTHKNPKAGQVWRPEATR SEG PRD ccccccccccceeecccccccccccccccccccccccccccccccccccccccc Prosite for DKFZphtes3_8gll .2 PS00017 839->847 ATP_GTP_A PDOC00017 (No Pfam data available for DKFZphtes3_8gll .2) DKFZphtes3_8g5 group: testes derived DKFZphtes3_8g5 encodes a novel 544 ammo acid protein nearly identical to human KIAA087 protein. The novel protein is a new splice variant of KIAA087. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . KIAA087, alternative spliced complete cDNA, complete eds, EST hits Sequenced by MediGenomix Locus : unknown Insert length: 2762 bp No poly A stretch found, no polyadenylation signal found 1 CCGACATCGG CCGTGTCTCC AGCACCTGCC GGCGGCTGCG CGAGCTGTGC 51 CAGAGCAGCG GGAAGGTGTG GAAGGAGCAG TTCCGGGTGA GGTGACCTTC 101 CCTTATGAAA CACTACAGCC CCACCGACTA CGTCAATTGG TTGGAAGAGT 151 ATAAAGTTCG GCAAAAAGCT GGGTTAGAAG CGCGGAAGAT TGTAGCCTCG 201 TTCTCAAAGA GGTTCTTTTC AGAGCACGTT CCTTGTAATG GCTTCAGTGA 251 CATTGAGAAC CTTGAAGGAC CAGAGATTTT TTTTGAGGAT GAACTGGTGT 301 GTATCCTAAA TATGGAAGGA AGAAAAGCTT TGACCTGGAA ATACTACGCA 351 AAAAAAATTC TTTACTACCT GCGGCAACAG AAGATCTTAA ATAATCTTAA 401 GGCCTTTCTT CAGCAGCCAG ATGACTATGA GTCGTATCTT GAAGGTGCTG 451 TATATATTGA CCAGTACTGC AATCCTCTCT CCGACATCAG CCTCAAAGAC 501 ATCCAGGCCC AAATTGACAG CATCGTGGAG CTTGTTTGCA AAACCCTTCG 551 GGGCATAAAC AGTCGCCACC CCAGCTTGGC CTTCAAGGCA GGTGAATCAT 601 CCATGATAAT GGAAATAGAA CTCCAGAGCC AGGTGCTGGA TGCCATGAAC 651 TATGTCCTTT ACGACCAACT GAAGTTCAAG GGGAATCGAA TGGATTACTA 701 TAATGCCCTC AACTTATATA TGCATCAGGT TTTGATTCGC AGAACAGGAA 751 TCCCAATCAG CATGTCTCTG CTCTATTTGA CAATTGCTCG GCAGTTGGGA 801 GTCCCACTGG AGCCTGTCAA CTTCCCAAGT CACTTCTTAT TAAGGTGGTG 851 CCAAGGCGCA GAAGGGGCGA CCCTGGACAT CTTTGACTAC ATCTACATAG 901 ATGCTTTTGG GAAAGGCAAG CAGCTGACAG TGAAAGAATG CGAGTACTTG 951 ATCGGCCAGC ACGTGACTGC AGCACTGTAT GGGGTGGTCA ATGTCAAGAA 1001 GGTGTTACAG AGAATGGTGG GAAACCTGTT AAGCCTGGGG AAGCGGGAAG 1051 GCATCGACCA GTCATACCAG CTCCTGAGAG ACTCGCTGGA TCTCTATCTG 1101 GCAATGTACC CGGACCAGGT GCAGCTTCTC CTCCTCCAAG CCAGGCTTTA 1151 CTTCCACCTG GGAATCTGGC CAGAGAAGTC TTTCTGTCTT GTTTTGAAGG 1201 TGCTTGACAT CCTCCAGCAC ATCCAAACCC TAGACCCGGG GCAGCACGGG 1251 GCGGTGGGCT ACCTGGTGCA GCACACTCTA GAGCACATTG AGCGCAAAAA 1301 GGAGGAGGTG GGCGTAGAGG TGAAGCTGCG CTCCGATGAG AAGCACAGAG 1351 ATGTCTGCTA CTCCATCGGG CTCATTATGA AGCATAAGAG GTATGGCTAT 1401 AACTGTGTGA TCTACGGCTG GGACCCCACC TGCATGATGG GACACGAGTG 1451 GATCCGGAAC ATGAACGTCC ACAGCCTGCC GCACGGCCAC CACCAGCCTT 1501 TCTATAACGT GCTGGTGGAG GACGGCTCCT GTCGATACGC AGCCCAAGAA 1551 AACTTGGAAT ATAACGTGGA GCCTCAAGAA ATCTCACACC CTGACGTGGG 1601 ACGCTATTTC TCAGAGTTTA CTGGCACTCA CTACATCCCA AACGCAGAGC 1651 TGGAGATCCG GTATCCAGAA GATCTGGAGT TTGTCTATGA AACGGTGCAG 1701 AATATTTACA GTGCAAAGAA AGAGAACATA GATGAGTAAA GTCTAGAGAG 1751 GACATTGCAC CTTTGCTGCT GCTGCTATCT TCCAAGAGAA CGGGACTCCG 1801 GAAGAAGACG TCTCCACGGA GCCCTCGGGA CCTGCTGCAC CAGGAAAGCC 1851 ACTCCACCAG TAGTGCTGGT TGCCTCCTAC TAAGTTTAAA TACCGTGTGC 1901 TCTTCCCCAG CTGCAAAGAC AATGTTGCTC TCCGCCTACA CTAGTGAATT 1951 AATCTGAAAG GCACTGTGTC AGTGGCATGG CTTGTATGCT TGTCCTGTGG 2001 TGACAGTTTG TGACATTCTG TCTTCATGAG GTCTCACAGT CGACGCTCCT 2051 GTAATCATTC TTTGTATTCA CTCCATTCCC CTGTCTGTCT GCATTTGTCT 2101 CAGAACATTT CCTTGGCTGG ACAGATGGGG TTATGCATTT GCAATAATTT 2151 CCTTCTGATT TCTCTGTGGA ACGTGTTCGG TCCCGAGTGA GGACTGTGTG 2201 TCTTTTTACC CTGAAGTTAG TTGCATATTC AGAGGTAAAG TTGTGTGCTA 2251 TCTTGGCAGC ATCTTAGAGA TGGAGACATT AACAAGCTAA TGGTAATTAG 2301 AATCATTTGA ATTTATTTTT TTCTAATATG TGAAACACAG ATTTCAAGTG 2351 TTTTATCTTT TTTTTTTTTA AATTTAAATG GGAATATAAC ACAGTTTTCC 2401 CTTCCATATT CCTCTCTTGA GTTTATGCAC ATCTCTATAA ATCATTAGTT 2451 TTCTATTTTA TTACATAAAA TTCTTTTAGA AAATGCAAAT AGTGAACTTT 2501 GTGAATGGAT TTTTCCATAC TCATCTACAA TTCCTCCATT TTAAATGACT 2551 ACTTTTATTT TTTAATTTAA AAAATCTACT TCAGTATCAT GAGTAGGTCT 2601 TACATCAGTG ATGGGTTCTT TTTGTAGTGA GACATACAAA TCTGATGTTA 2651 ATGTTTGCTC TTAGAAGTCA TACTCCATGG TCTTCAAAGA CCAAAAAATG 2701 AGGTTTTGCT TTTGTAATCA GGAAAAAAAA AATTAATGAA CCTTAAAAAA 2751 AAAAAAAAAA GG BLAST Results No BLAST result Medline entries No Medline entry Peptide information for frame 3 ORF from 105 bp to 1736 bp; peptide length: 544 Category: known protein Classification: unclassified 1 MKHYSPTDYV NWLEEYKVRQ KAGLEARKIV ASFSKRFFSE HVPCNGFSDI 51 ENLEGPEIFF EDELVCILNM EGRKALTWKY YAKKILYYLR QQKILNNLKA 101 FLQQPDDYES YLEGAVYIDQ YCNPLSDISL KDIQAQIDSI VELVCKTLRG 151 INSRHPSLAF KAGESSMIME IELQSQVLDA MNYVLYDQLK FKGNRMDYYN 201 ALNLYMHQVL IRRTGIPISM SLLYLTIARQ LGVPLEPVNF PSHFLLRWCQ 251 GAEGATLDIF DYIYIDAFGK GKQLTVKECE YLIGQHVTAA LYGVVNVKKV 301 LQRMVGNLLS LGKREGIDQS YQLLRDSLDL YLAMYPDQVQ LLLLQARLYF 351 HLGIWPEKSF CLVLKVLDIL QHIQTLDPGQ HGAVGYLVQH TLEHIERKKE 401 EVGVEVKLRS DEKHRDVCYS IGLIMKHKRY GYNCVIYGWD PTCMMGHEWI 451 RNMNVHSLPH GHHQPFYNVL VEDGSCRYAA QENLEYNVEP QEISHPDVGR 501 YFSEFTGTHY IPNAELEIRY PEDLEFVYET VQNIYSAKKE NIDE BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_8g5, frame 3 TREMBLNEW:AB020682_1 gene: "KIAA0875"; product: "KIAA0875 protein"; Homo sapiens mRNA for KIAA0875 protein, partial eds., N = 1, Score = 2832, P = 5.5e-295 >TREMBLNEW:AB020682_1 gene: "KIAA0875"; product: "KIAA0875 protein"; Homo sapiens mRNA for KIAA0875 protein, partial eds. Length = 621 HSPs: Score = 2832 (424.9 bits). Expect = 5.5e-295, P = 5.5e-295 Identities = 537/544 (98%), Positives = 537/544 (98%) Query: 1 MKHYSPTDYVNWLEEYKVRQKAGLEARKIVASFSKRFFSEHVPCNGFSDIENLEGPEIFF 60 MKHYSPTDYVNWLEEYKVRQKAGLEARKIVASFSKRFFSEHVPCNGFSDIENLEGPEIFF Sbjct: 85 MKHYSPTDYVNWLEEYKVRQKAGLEARKIVASFSKRFFΞEHVPCNGFSDIENLEGPEIFF 144 Query: 61 EDELVCILNMEGRKALTWKYYAKKILYYLRQQKILNNLKAFLQQPDDYESYLEGAVYIDQ 120 EDELVCILNMEGRKALTWKYYAKKILYYLRQQKILNNLKAFLQQPDDYESYLEGAVYIDQ Sbjct: 145 EDELVCILNMEGRKALTWKYYAKKILYYLRQQKILNNLKAFLQQPDDYESYLEGAVYIDQ 204 Query: 121 YCNPLSDISLKDIQAQIDSIVELVCKTLRGINSRHPSLAFKAGESSMIMEIELQSQVLDA 180 YCNPLSDISLKDIQAQIDSIVELVCKTLRGINSRHPSLAFKAGESSMIMEIELQSQVLDA Sbjct: 205 YCNPLSDISLKDIQAQIDSIVELVCKTLRGINSRHPΞLAFKAGESSMIMEIELQSQVLDA 264 Query: 181 MNYVLYDQLKFKGNRMDYYNALNLYMHQVLIRRTGIPISMSLLYLTIARQLGVPLEPVNF 240 MNYVLYDQLKFKGNRMDYYNALNLYMHQVLIRRTGIPISMSLLYLTIARQLGVPLEPVNF Sbjct: 265 MNYVLYDQLKFKGNRMDYYNALNLYMHQVLIRRTGIPISMSLLYLTIARQLGVPLEPVNF 324 Query: 241 PSHFLLRWCQGAEGATLDIFDYIYIDAFGKGKQLTVKECEYLIGQHVTAALYGVVNVKKV 300 PSHFLLRWCQGAEGATLDIFDYIYIDAFGKGKQLTVKECEYLIGQHVTAALYGVVNVKKV Sb j ct: 325 PSHFLLRWCQGAEGATLDIFDYIYIDAFGKGKQLTVKECEYLIGQHVTAALYGVVNVKKV 384 Query: 301 LQRMVGNLLSLGKREGIDQSYQLLRDSLDLYLAMYPDQVQLLLLQARLYFHLGIWPEKSF 360 LQRMVGNLLSLGKREGIDQSYQLLRDSLDLYLAMYPDQVQLLLLQARLYFHLGIWPEK Sbjct: 385 LQRMVGNLLSLGKREGIDQSYQLLRDSLDLYLAMYPDQVQLLLLQARLYFHLGIWPEK— 442 Query: 361 CLVLKVLDILQHIQTLDPGQHGAVGYLVQHTLEHIERKKEEVGVEVKLRSDEKHRDVCYS 420 VLDILQHIQTLDPGQHGAVGYLVQHTLEHIERKKEEVGVEVKLRSDEKHRDVCYS Sbjct: 443 VLDILQHIQTLDPGQHGAVGYLVQHTLEHIERKKEEVGVEVKLRSDEKHRDVCYS 497 Query: 421 IGLIMKHKRYGYNCVIYGWDPTCMMGHEWIRNMNVHSLPHGHHQPFYNVLVEDGSCRYAA 480 IGLIMKHKRYGYNCVIYGWDPTCMMGHEWIRNMNVHSLPHGHHQPFYNVLVEDGSCRYAA Sbjct: 498 IGLIMKHKRYGYNCVIYGWDPTCMMGHEWIRNMNVHSLPHGHHQPFYNVLVEDGSCRYAA 557 Query: 481 QENLEYNVEPQEISHPDVGRYFSEFTGTHYIPNAELEIRYPEDLEFVYETVQNIYSAKKE 540 QENLEYNVEPQEISHPDVGRYFSEFTGTHYIPNAELEIRYPEDLEFVYETVQNIYSAKKE Sbjct: 558 QENLEYNVEPQEISHPDVGRYFΞEFTGTHYIPNAELEIRYPEDLEFVYETVQNIYSAKKE 617 Query: 541 NIDE 544 NIDE Sbjct: 618 NIDE 621 Pedant information for DKFZphtes3_8g5, frame 3 Report for DKFZphtes3_8g5.3 [LENGTH] 544 [MW] 63307.22 [pi] 5.82 [HOMOL] TREMBL:AB020682_1 gene: "KIAA0875"; product: "KIAA0875 protein"; Homo sapiens mRNA for KIAA0875 protein, partial eds. 0.0 [KW] Alpha_Beta [KW] LOW_COMPLEXITY 1.84 % SEQ MKHYSPTDYVNWLEEYKVRQKAGLEARKIVASFSKRFFSEHVPCNGFSDIENLEGPEIFF SEG PRD cccccccccchhhhhhhhhhhhhchhhhhhhhhhhhhhhcccccccccccccccccceee SEQ EDELVCILNMEGRKALTWKYYAKKILYYLRQQKILNNLKAFLQQPDDYESYLEGAVYIDQ SEG PRD eeeeeeeeeeccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccccceeecceeeeeee SEQ YCNPLSDISLKDIQAQIDSIVELVCKTLRGINSRHPSLAFKAGESSMIMEIELQSQVLDA SEG PRD ccccccccchhhhhhhhhhhhhhhhhhcccccccccceeeecccchhhhhhhhhhhhhhh SEQ MNYVLYDQLKFKGNRMDYYNALNLYMHQVLIRRTGIPISMSLLYLTIARQLGVPLEPVNF SEG PRD hhhhhccccccccccchhhhhhhhhhhhhhhhhccccchhhhhhhhhhhhcccccccccc SEQ PSHFLLRWCQGAEGATLDIFDYIYIDAFGKGKQLTVKECEYLIGQHVTAALYGVVNVKKV SEG PRD cceeeeeeccccccceeeeeeeeeeeccccceeeeeehhhhhhhhhhhhhhhhhhhhhhh SEQ LQRMVGNLLSLGKREGIDQSYQLLRDSLDLYLAMYPDQVQLLLLQARLYFHLGIWPEKSF SEG PRD hhhhhccchhhhhhhhccccccchhhhhhhhhhhccchhhhhhhhhhhhhhcccccceee SEQ CLVLKVLDILQHIQTLDPGQHGAVGYLVQHTLEHIERKKEEVGVEVKLRSDEKHRDVCYS SEG xxxxxxxxxx PRD ehhhhhhhhhhhhhccccccccchhhhhhhhhhhhhhhhhhhheeeeecccccceeeecc SEQ IGLIMKHKRYGYNCVIYGWDPTCMMGHEWIRNMNVHSLPHGHHQPFYNVLVEDGSCRYAA SEG PRD cccchhhhhhhceeeeecccccccchhhhhhhhhhhccccccccccceeeeecccceeee SEQ QENLEYNVEPQEISHPDVGRYFSEFTGTHYIPNAELEIRYPEDLEFVYETVQNIYSAKKE SEG PRD hhhhhhhhcccccccccceeeeccccccccccchhhhhhccchhhhhhhhhhhhhccccc SEQ NIDE SEG .... PRD CCCC (No Prosite data available for DKFZphtes3_8g5.3) (No Pfam data available for DKFZphtes3_8g5.3) DKFZphtes3_8mlO group: nucleic acid management DKFZphtes3_8mlO encodes a novel 221 amino acid protein with strong similarity to polyadenylate-binding proteins. The poly (A) -binding protein (PABP) binds to the messenger (mRNA) 3 '-poly (A) tail found on most eukaryotic raRNAs and together with the poly (A) tail has been implicated in governing the stability and the translation of mRNA. The new protein can find application in modulation of mRNA translation and processing/stability. strong similarity to polyadenylate-binding protein frame shift at Bp 707-710 Sequenced by MediGenomix Locus : unknown Insert length: 2107 bp Poly A stretch at pos. 2052, polyadenylation signal at pos. 2033 1 CGGAAAGGTC GCGGCTTGTG TGCCTGCGGG CAGCCGTGCC GAGAATGAAC 51 CCCAGCACCC CCAGCTACCC AACGGCCTCG CTCTACGTGG GGGACCTCCA 101 CCCCGACGTG ACTGAGGCGA TGCTCTACGA GAAGTTCAGC CCGGCAGGGC 151 CCATCCTCTC CATCCGGATC TGCAGGGACT TGATCACCAG CGGCTCCTCC 201 AACTACGCGT ATGTGAACTT CCAGCATACG AAGGACGCGG AGCATGCTCT 251 GGACACCATG AATTTTGATG TTATAAAGGG CAAGCCAGTA CGCATCATGT 301 GGTCTCAGCG TGATCCATCA CTTCGAAAAA GTGGAGTGGG CAACATATTC 351 GTTAAAAATC TGGATAAGTC CATTAATAAT AAAGCACTGT ATGATACAGT 401 TTCTGCTTTT GGTAACATCC TTTCGTGTAA CGTGGTTTGT GATGAAAATG 451 GTTCCAAGGG TTATGGATTT GTACACTTTG AGACACACGA AGCAGCTGAA 501 AGAGCTATTA AAAAAATGAA CGGAATGCTC CTAAATGGTC GCAAAGTATT 551 TGTTGGACAA TTTAAGTCTC GTAAAGAACG AGAAGCTGAA CTTGGAGCTA 601 GGGCAAAAGA GTTCCCCAAT GTTTACATCA AGAATTTTGG AGAAGACATG 651 GATGATGAGC GCCTTAAGGA TCTCTTTGGC AAGTTCGGGC CCGCCTTAAG 701 TGTGAATTAA TGACCGATGA AAGTGGAAAA TCCAAAGGAT TTGGATTTGT 751 AAGCTTTGAA AGGCATGAAG ATGCACAGAA AGCTGTAGAT GAGATGAATG 801 GAAAGGAGCT CAATGGAAAA CAAATTTACG TTGGTCGAGC TCAGAAAAAA 851 GTGGAACGGC AGACGGAACT TAAGCGCACA TTTGAACAGA TGAAGCAAGA 901 TAGGATCACC AGATACCAGG TTGTTAATCT TTATGTGAAA AATCTTGATG 951 ATGGTATTGA TGATGAACGT CTCCGGAAAG CGTTTTCTCC ATTTGGTACA 1001 ATCACTAGTG CAAAGGTTAT GATGGAAGGT GGTCGCAGCA AAGGGTTTGG 1051 TTTTGTATGT TTCTCCTCCC CAGAAGAAGC CACTAAAGCA GTTACAGAAA 1101 TGAACGGTAG AATTGTGGCC ACAAAGCCAT TGTATGTAGC TTTAGCTCAG 1151 CGCAAAGAAG AGCGCCAGGC TTACCTCACT AACGAGTATA TGCAGAGAAT 1201 GGCAAGTGTA CGAGCTGTGC CCAACCAGCG AGCACCTCCT TCAGGTTACT 1251 TCATGACAGC TGTCCCACAG ACTCAGAACC ATGCTGCATA CTATCCTCCT 1301 AGCCAAATTG CTCGACTAAG ACCAAGTCCT CGCTGGACTG CTCAGGGTGC 1351 CAGACCTCAT CCATTCCAAA ATAAGCCCAG TGCTATCCGC CCAGGTGCTC 1401 CTAGAGTACC ATTTAGTACT ATGAGACCAG CTTCTTCACA GGTTCCACGA 1451 GTCATGTCAA CGCAGCGTGT TGCTAACACA TCAACACAGA CAGTGGGTCC 1501 ACGTCCTGCA GCTGCTGCTG CTGCTGCAGC TACCCCTGCT GTGCGCACGG 1551 TTCCACGGTA TAAATATGCT GCGGGAGTTC GCAATCCTCA GCAACATCGT 1601 AATGCACAGC CACAAGTTAC AATGCAACAG CTTGCTGTTC ATGTACAAGG 1651 TCAGGAAACT TTGACTGCCT CCAGGTTGGC ATCTGCCCCT CCTCAAAAGC 1701 AAAAGCAAAT GTTAGGTGAA CGGCTCTTTC CTCTTATTCA AGCCATGCAC 1751 CCTACTCTTG CTGGGAAAAT CACTGGCATG TTGTTGGAGA TTGATAATTC 1801 AGAACTTCTT TATATGCTCG AGTCTCCAGA GTCACTCCGT TCTAAGGTTG 1851 ATGAAGCTGT AGCTGTACTA CAAGCCCACC AAGCTAAAGA GGCTACCCAG 1901 AAAGCAGTTA ACAGTGCTAC CGGTGTTCCA ACTGTTTAAA ATTGATCAGA 1951 GACCACGAAA AGAAATTTGT GCTTCACCGA AGAAAAATAT CTAAACATCG 2001 AGAAACTATG GGAAAAAAAA TTGCAAAATC TAAAATAAAA AATGCAAAAT 2051 CTAAAATAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 2101 AAAAAGG BLAST Results Entry HSPOLYAB from database EMBL: Human mRNA for polyA binding protein Score = 5420, P = O.Oe+00, identities = 1162/1243 Medlme entries No Medline entry Peptide information for frame 2 ORF from 707 bp to 1936 bp; peptide length: 410 Category: strong similarity to known protein Classification: unset Prosite motifs: RNP_1 (10-18) RNP 1 (112-120) 1 LMTDESGKSK GFGFVSFERH EDAQKAVDEM NGKELNGKQI YVGRAQKKVE 51 RQTELKRTFE QMKQDRITRY QVVNLYVKNL DDGIDDERLR KAFSPFGTIT 101 SAKVMMEGGR SKGFGFVCFS SPEEATKAVT EMNGRIVATK PLYVALAQRK 151 EERQAYLTNE YMQRMASVRA VPNQRAPPSG YFMTAVPQTQ NHAAYYPPSQ 201 IARLRPSPRW TAQGARPHPF QNKPSAIRPG APRVPFSTMR PASSQVPRVM 251 STQRVANTST QTVGPRPAAA AAAAATPAVR TVPRYKYAAG VRNPQQHRNA 301 QPQVTMQQLA VHVQGQETLT ASRLASAPPQ KQKQMLGERL FPLIQAMHPT 351 LAGKITGMLL EIDNSELLYM LESPESLRSK VDEAVAVLQA HQAKEATQKA 401 VNSATGVPTV BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_8mlO, frame 2 PIR:DNHUPA polyadenylate-bindmg protein - human, N = 1, Score = 1931, P = 1.7e-199 PIR:I48718 poly(A) binding protein - mouse, N = 1, Score = 1928, P = 3.6e-199 >PIR:DNHUPA polyadenylate-binding protein - human Length = 633 HSPs: Score = 1931 (289.7 bits), Expect = 1.7e-199, P = 1.7e-199 Identities = 384/415 (92%), Positives = 394/415 (94%) Query: 1 LMTDESGKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIYVGRAQKKVERQTELKRTFE 60 +MTDESGKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIYVGRAQKKVERQTELKR FE Sbjct: 219 VMTDESGKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIYVGRAQKKVERQTELKRKFE 278 Query: 61 QMKQDRITRYQVVNLYVKNLDDGIDDERLRKAFSPFGTITSAKVMMEGGRSKGFGFVCFS 120 QMKQDRITRYQ VNLYVKNLDDGIDDERLRK FSPFGTITSAKVMMEGGRSKGFGFVCFS Sbjct: 279 QMKQDRITRYQGVNLYVKNLDDGIDDERLRKEFSPFGTITSAKVMMEGGRSKGFGFVCFS 338 Query: 121 SPEEATKAVTEMNGRIVATKPLYVALAQRKEERQAYLTNEYMQRMASVRAVPN Q 174 SPEEATKAVTEMNGRIVATKPLYVALAQRKEERQA+LTN+YMQRMASVRAVPN Q Sbjct: 339 SPEEATKAVTEMNGRIVATKPLYVALAQRKEERQAHLTNQYMQRMASVRAVPNPVINPYQ 398 Query: 175 RAPPSGYFMTAVPQTQNHAAYYPPSQIARLRPSPRWTAQGARPHPFQNKPSAIRPGAPRV 234 APPSGYFM A+PQTQN AAYYPPSQ+A+LRPSPRWTAQGARPHPFQN P AIRP APR Sbjct: 399 PAPPSGYFMAAIPQTQNRAAYYPPSQVAQLRPSPRWTAQGARPHPFQNMPGAIRPAAPRP 458 Query: 235 PFSTMRPASSQVPRVMSTQRVANTSTQTVGPRPAAAAAAAATPAVRTVPRYKYAAGVRNP 294 PFSTMRPASSQVPRVMSTQRVANTSTQT+GPRPAAAAAAA TPAVRTVP+YKYAAGVRNP Sbjct: 459 PFSTMRPASSQVPRVMSTQRVANTΞTQTMGPRPAAAAAAA-TPAVRTVPQYKYAAGVRNP 517 Query: 295 QQHRNAQPQVTMQQLAVHVQGQETLTAΞRLASAPPQKQKQMLGERLFPLIQAMHPTLAGK 354 QQH NAQPQVTMQQ AVHVQGQE LTAS LASAPPQ+QKQMLGERLFPLIQAMHPTLAGK Sbjct: 518 QQHLNAQPQVTMQQPAVHVQGQEPLTASMLASAPPQEQKQMLGERLFPLIQAMHPTLAGK 577 Query: 355 ITGMLLEIDNSELLYMLESPESLRSKVDEAVAVLQAHQAKEATQKAVNSATGVPTV 410 ITGMLLEIDNSELL+MLESPESLRSKVDEAVAVLQAHQAKEA QKAVNSATGVPTV Sb j ct: 578 ITGMLLEIDNSELLHMLESPESLRSKVDEAVAVLQAHQAKEAAQKAVNSATGVPTV 633 Score = 315 (47.3 bits), Expect = 1.9e-27, P = 1.9e-27 Identities = 71/163 (43%), Positives = 102/163 (62%) Query: 1 LMTDESGKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIYVGRAQKKVERQTELKRTFE 60 ++ DE+G SKG+GFV FE E A++A+++MNG LN ++++VGR + + ER+ EL + Sbjct: 130 VVCDENG-SKGYGFVHFETQEAAERAIEKMNGMLLNDRKVFVGRFKSRKEREAELGARAK 188 Query: 61 QMKQDRITRYQVVNLYVKNLDDGIDDERLRKAFSPFGTITSAKVMM-EGGRSKGFGFVCF 119 + N+Y+KN + +DDERL+ F P S KVM E G+ΞKGFGFV F Sbjct: 189 EF TNVYIKNFGEDMDDERLKDLFGP ALΞVKVMTDESGKSKGFGFVSF 235 Query: 120 SSPEEATKAVTEMNGRIVATKPLYVALAQRKEERQAYLTNEYMQ 163 E+A KAV EMNG+ + K +YV AQ+K ERQ L ++ Q Sbjct: 236 ERHEDAQKAVDEMNGKELNGKQIYVGRAQKKVERQTELKRKFEQ 279 Score = 214 (32.1 bits), Expect = 1.9e-14, P = 1.9e-14 Identities = 50/150 (33%), Positives = 87/150 (58%) Query: 8 KSKGFGFVΞFERHEDAQKAVDEMNGKELNGKQIYVGRAQKKVERQTELKRTFEQMKQDRI 67 +S G+ +V+F++ DA++A+D MN + GK + + +Q R L+++ Sbjct: 50 RSLGYAYVNFQQPADAERALDTMNFDVIKGKPVRIMWSQ RDPSLRKS 96 Query: 68 TRYQVVNLYVKNLDDGIDDERLRKAFSPFGTITSAKVMMEGGRSKGFGFVCFSSPEEATK 127 V N+++KNLD ID++ L FS FG I S KV+ + SKG+GFV F + E A + Sbjct: 97 GVGNIFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGSKGYGFVHFETQEAAER 153 Query: 128 AVTEMNGRIVATKPLYVALAQRKEERQAYL 157 A+ +MNG ++ + ++V + ++ER+A L Sbjct: 154 AIEKMNGMLLNDRKVFVGRFKSRKEREAEL 183 Score = 120 (18.0 bits), Expect = 4.8e-04, P = 4.8e-04 Identities = 30/99 (30%), Positives = 54/99 (54%) Query: 70 YQVVNLYVKNLDDGIDDERLRKAFSPFGTITSAKVM—MEGGRSKGFGFVCFSSPEEATK 127 Y + +LYV +L + + L + FSP G I S +V M RS G+ +V F P +A + Sbjct: 8 YPMASLYVGDLHPDVTEAMLYEKFSPAGPILSIRVCRDMITRRSLGYAYVNFQQPADAER 67 Query: 128 AVTEMNGRIVATKPLYVALAQRKEE-RQAYLTNEYMQRM 165 A+ MN ++ KP+ + +QR R++ + N +++ + Sbjct: 68 ALDTMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIFIKNL 106 Peptide information for frame 3 ORF from 45 bp to 707 bp; peptide length: 221 Category: strong similarity to known protein Classification: unset Prosite motifs: RNP 1 (138-146) 1 MNPSTPSYPT ASLYVGDLHP DVTEAMLYEK FSPAGPILSI RICRDLITSG 51 SSNYAYVNFQ HTKDAEHALD TMNFDVIKGK PVRIMWSQRD PSLRKSGVGN 101 IFVKNLDKSI NNKALYDTVS AFGNILSCNV VCDENGSKGY GFVHFETHEA 151 AERAIKKMNG MLLNGRKVFV GQFKSRKERE AELGARAKEF PNVYIKNFGE 201 DMDDERLKDL FGKFGPALSV N BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_8mlO, frame 3 SWISSPROT :PAB1_HUMAN POLYADENYLATE-BINDING PROTEIN 1 (POLY (A) BINDING PROTEIN 1) (PABP 1)., N = 1, Score = 1039, P = 5.7e-105 PIR:I48718 poly(A) binding protein - mouse, N = 1, Score = 1031, P = 4e-104 PIR:DNHUPA polyadenylate-binding protein - human, N = 1, Score = 1009, P = 8.7e-102 >SWISSPROT:PABl_HUMAN POLYADENYLATE-BINDING PROTEIN 1 (POLY (A) BINDING PROTEIN 1) (PABP 1) . Length = 636 HSPs: Score = 1039 (155.9 bits), Expect = 5.7e-105, P = 5.7e-105 Identities = 199/220 (90%), Positives = 205/220 (93%) Query: 1 MNPSTPSYPTASLYVGDLHPDVTEAMLYEKFSPAGPILSIRICRDLITSGSSNYAYVNFQ 60 MNPS PSYP ASLYVGDLHPDVTEAMLYEKFSPAGPILSIR+CRD+IT S YAYVNFQ Sb j ct: 1 MNPSAPSYPMASLYVGDLHPDVTEAMLYEKFSPAGPILSIRVCRDMITRRSLGYAYVNFQ 60 Query: 61 HTKDAEHALDTMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIFVKNLDKSINNKALYDTVS 120 DAE ALDTMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIF+KNLDKSI+NKALYDT S Sbjct: 61 QPADAERALDTMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIFIKNLDKSIDNKALYDTFS 120 Query: 121 AFGNILSCNVVCDENGSKGYGFVHFETHEAAERAIKKMNGMLLNGRKVFVGQFKSRKERE 180 AFGNILSC VVCDENGSKGYGFVHFET EAAERAI+KMNGMLLN RKVFVG+FKSRKERE Sbjct: 121 AFGNILSCKVVCDENGSKGYGFVHFETQEAAERAIEKMNGMLLNDRKVFVGRFKSRKERE 180 Query: 181 AELGARAKEFPNVYIKNFGEDMDDERLKDLFGKFGPALSV 220 AELGARAKEF NVYIKNFGEDMDDERLKDLFGKFGPALSV Sbjct: 181 AELGARAKEFTNVYIKNFGEDMDDERLKDLFGKFGPALSV 220 Score = 275 (41.3 bits), Expect = 4. le-23, P = 4. le-23 Identities = 71/233 (30%), Positives = 120/233 (51%) Query: 2 NPSTPSYPTASLYVGDLHPDVTEAMLYEKFSPAGPILSIRICRDLITSGSSNYAYVNFQH 61 +PS ++++ +L + LY+ FS G ILS ++ D S + + Q Sbjct: 90 DPSLRKSGVGNIFIKNLDKSIDNKALYDTFSAFGNILΞCKVVCDENGSKGYGFVHFETQE 149 Query: 62 TKD-AEHALDTMNFDVIKGKPVRIMW-SQRDPSL--RKSGVGNIFVKNLDKSINNKALYD 117 + A ++ M + K R +R+ L R N+++KN + ++++ L D Sbjct: 150 AAERAIEKMNGMLLNDRKVFVGRFKSRKEREAELGARAKEFTNVYIKNFGEDMDDERLKD 209 Query: 118 TVSAFGNILSCNVVCDENG-SKGYGFVHFETHEAAERAIKKMNGMLLNGRKVFVGQFKSR 176 FG LS V+ DE+G SKG+GFV FE HE A++A+ +MNG LNG++++VG+ + + Sbjct: 210 LFGKFGPALSVKVMTDESGKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIYVGRAQKK 269 Query: 177 KEREAELGARAKEFP NVYIKNFGEDMDDERLKDLFGKFGPALS 219 ER+ EL + ++ N+Y+KN + +DDERL+ F FG S Sbjct: 270 VERQTELKRKFEQMKQDRITRYQGVNLYVKNLDDGIDDERLRKEFSPFGTITS 322 Score = 227 (34.1 bits), Expect = 6.3e-18, P = 6.3e-18 Identities = 57/187 (30%), Positives = 101/187 (54%) Query: 12 SLYVGDLHPDVTEAMLYEKFSPAGPILSIRICRDLITSGSSNYAYVNFQHTKDAEHALDT 71 ++Y+ + D+ + L + F GP LS+++ D + S + +V+F+ +DA+ A+D Sbjct: 192 NVYIKNFGEDMDDERLKDLFGKFGPALSVKVMTDE-SGKSKGFGFVSFERHEDAQKAVDE 250 Query: 72 MNFDVIKGKPVRIMWSQR DPSLRKSGVGNIFVKNLDKSINNKA 114 MN + GK + + +Q+ D R GV N++VKNLD I+++ Sbjct: 251 MNGKELNGKQIYVGRAQKKVERQTELKRKFEQMKQDRITRYQGV-NLYVKNLDDGIDDER 309 Query: 115 LYDTVSAFGNILSCNVVCDENGSKGYGFVHFETHEAAERAIKKMNGMLLNGRKVFVGQFK 174 L S FG I S V+ + SKG+GFV F + E A +A+ +MNG ++ + ++V + Sbjct: 310 LRKEFSPFGTITSAKVMMEGGRSKGFGFVCFSSPEEATKAVTEMNGRIVATKPLYVALAQ 369 Query: 175 SRKEREAEL 183 ++ER+A L Sbjct: 370 RKEERQAHL 378 Score = 100 (15.0 bits), Expect = 2.3e-02, P = 2.3e-02 Identities = 26/99 (26%), Positives = 53/99 (53%) Query: 8 YPTASLYVGDLHPDVTEAMLYEKFSPAGPILSIRICRDLITSG-SSNYAYVNFQHTKDAE 66 Y +LYV +L + + L ++FSP G I S ++ ++ G S + +V F ++A Sbjct: 291 YQGVNLYVKNLDDGIDDERLRKEFSPFGTITSAKV MMEGGRSKGFGFVCFSSPEEAT 347 Query: 67 HALDTMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIFVKNL 106 A+ MN ++ KP+ + +QR R++ + N +++ + Sbjct: 348 KAVTEMNGRIVATKPLYVALAQRKEE-RQAHLTNQYMQRM 386 Pedant information for DKFZphtes3_8mlO, frame 2 Report for DKFZphtes3_8mlO .2 [LENGTH] 409 [MW] 45235.68 [pi] 10.08 [HOMOL] SWISSPROT :PAB1_HUMAN POLYADENYLATE-BINDING PROTEIN 1 (POLY (A) BINDING PROTEIN 1) (PABP 1) . 0.0 [FUNCAT] 04.05.05 mrna processing (5 '-end, 3 '-end processing and mrna degradation) [S. cerevisiae, YER165w] le-54 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YER165w] le-54 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YER165w] le-54 [FUNCAT] 05.04 translation (initiation, elongation and termination) [S. cerevisiae, YER165w] le -54 [FUNCAT] 04.05.99 other mrna-transcπption activities [S. cerevisiae, YNL016w] le-15 [FUNCAT] 11.01 stress response [S. cerevisiae, YGR159c] le-12 [ FUNCAT ] 04.01.04 rrna processing [S. cerevisiae, YGR159c) le-12 [ FUNCAT ] 04.99 other transcription activities [S. cerevisiae, YNL175C] 4e-09 [ FUNCAT ] 98 classification not yet clear-cut [S. cerevisiae, YPR112c] 5e-08 [FUNCAT] 03.19 recombination and dna repair [S. cerevisiae, YHR086w] 3e-07 [FUNCAT] 03.13 meiosis [S. cerevisiae, YHR086w] 3e-07 [FUNCAT] 04.05.03 mrna processing (splicing) [S. cerevisiae, YHR086w] 3e-07 [FUNCAT] 04.07 rna transport [S. cerevisiae, YOL12 3 w HRPl - CF Ib] 9e-07 [FUNCAT] 30.13 organization of chromosome structure [S. cerevisiae, YCLOllc] 3e-06 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YGR250c] 8e-06 [FUNCAT] 06.04 protein targeting, sorting and translocation [S. cerevisiae, YDR432w] 2e-05 [FUNCAT] 08.01 nuclear transport [S. cerevisiae, YDR432w] 2e-05 [FUNCAT] 11.04 dna repair (direct repair, base excision repair and nucleotide excision repair) [Ξ. cerevisiae, YFR023w] 3e-05 [FUNCAT] 03.01 cell growth [S. cerevisiae, YBR212w] 3e-04 [BLOCKS] BL00030B Eukaryotic RNA-binding region RNP-1 proteins [SCOP] dlsxl 4.34.7.1.3 Sex-lethal protein [(Drosophila melanogaster) le-17 [PIRKW] nucleus 0.0 [PIRKW] duplication 0.0 [PIRKW] RNA binding 0.0 [PIRKW] nucleolus 2e-09 [PIRKW] tandem repeat 2e-09 [PIRKW] single-stranded DNA binding 3e-06 [PIRKW] DNA binding 5e-13 [PIRKW] phosphoprotem 6e-10 [PIRKW] ribosome 3e-08 [PIRKW] mitochondrion 3e-08 [PIRKW] alternative splicing 9e-ll [PIRKW] chloroplast 2e-19 [PIRKW] transcription regulation 2e-07 [PIRKW] protein biosynthesis 3e-08 [SUPFAM] nucleolm 6e-10 [SUPFAM] glycine-rich RNA-binding protein 2e-07 [SUPFAM] unassigned ribonucleoprotein repeat-containing proteins 2e-19 [SUPFAM] polyadenylate-binding protein 0.0 [SUPFAM] ribonucleoprotein repeat homology 0.0 [PROSITE] RNP_1 2 [PFAM] RNA recognition motif, (aka RRM, RBD, or RNP domain) [KW] Irregular [KW] 3D [KW] LOW COMPLEXITY 5.62 % SEQ MTDESGKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIYVGRAQKKVERQTELKRTFEQ SEG lsxl- SEQ MKQDRITRYQVVNLYVKNLDDGIDDERLRKAFSPFGTITSAKVMMEGGRSKGFGFVCFSS SEG lsxl- CEEEECCCTTTTHHHHHHHHTTTTCCCCCEEECTTTCTTTEEEECTTT SEQ PEEATKAVTEMNGRIVATKPLYVALAQRKEERQAYLTNEYMQRMASVRAVPNQRAPPSGY SEG lsxl- HHHHHHHHHHHTTTCCCCCCCBCCBCC SEQ FMTAVPQTQNHAAYYPPSQIARLRPSPRWTAQGARPHPFQNKPSAIRPGAPRVPFSTMRP SEG lsxl- SEQ ASSQVPRVMSTQRVANTSTQTVGPRPAAAAAAAATPAVRTVPRYKYAAGVRNPQQHRNAQ SEG xxxxxxxxxxxxxxxxxxxxxxx lsxl- SEQ PQVTMQQLAVHVQGQETLTASRLASAPPQKQKQMLGERLFPLIQAMHPTLAGKITGMLLE SEG lsxl- SEQ IDNSELLYMLESPESLRSKVDEAVAVLQAHQAKEATQKAVNSATGVPTV SEG lsxl- Prosite for DKFZphtes3_8mlO .2 PS00030 9->17 RNP_ PDOC00030 PS00030 111->119 RNP PDOC00030 Pfam for DKFZphtes3_8ml0.2 HMM_NAME RNA recognition motif, (aka RRM, RBD, or RNP domain) HMM *IYVGNLPWDtTEEDLrDlFsQFGpIvsIrMMrDReTGRSRGFAFVEFED +YV+NL+ +++E LR +FS+FG I+S+++M+ E GRS+GF+FV F + Query 74 LYVKNLDDGIDDERLRKAFSPFGTITSAKVM —EGGRSKGFGFVCFSS 120 HMM EEDAekAIdeMNGmeFmGRrlRV* +E+A+KA+ EMNG+++ ++++V Query 121 PEEATKAVTEMNGRIVATKPLYV 143 Pedant information for DKFZphtes3_8ml0, frame 3 Report for DKFZphtes3_8mlO .3 [LENGTH] 235 [MW] 26308.08 [pi] 8.95 [HOMOL] SWISSPROT :PAB1_HUMAN POLYADENYLATE-BINDING PROTEIN 1 (POLY (A) BINDING PROTEIN 1) (PABP 1) , le-113 [FUNCAT] 04.05.05 mrna processing (5 '-end, 3 ' -end processing and mrna degradation) [S. cerevisiae, YER165w] le-64 [FUNCAT] 30.03 organization of cytoplasm [S. cerevisiae, YER165w] le-64 [FUNCAT) 05.04 translation (initiation, elongation and termination) [S. cerevisiae. YER165w] le -64 [FUNCAT] 30.10 nuclear organization [S. cerevisiae, YER165w] le-64 [FUNCAT] 03.19 recombination and dna repair [S. cerevisiae, YFR023w] le-24 [FUNCAT] 11.04 dna repair (direct repair, base excision repair and nucleotide excision repair) [S. cerevisiae, YFR023w] le-24 [FUNCAT] 04.05.99 other mrna-transcπption activities [S. cerevisiae, YNL016w] 2e-19 [ FUNCAT] 04.05.03 mrna processing (splicing) [S. cerevisiae, YOR319w] 2e-14 [FUNCAT] 04.01.04 rrna processing [S. cerevisiae, YGR159c] le-11 [FUNCAT] 11.01 stress response [S. cerevisiae, YGR159c] le-11 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YGR250c] le-09 [FUNCAT] 04.07 rna transport [S. cerevisiae, YOL123w HRP1 - CF Ib] le-09 [FUNCAT] 30.13 organization of chromosome structure [S. cerevisiae, YCLOllc] 8e-09 [FUNCAT] 98 classification not yet clear-cut [S. cerevisiae, YPR112c] 2e-08 [FUNCAT] 03.13 meiosis [S. cerevisiae, YHR086w] 2e-08 [FUNCAT] 04.99 other transcription activities [S. cerevisiae, YBR212w] 3e-08 [FUNCAT] 03.01 cell growth [S. cerevisiae, YBR212w] 3e-08 [FUNCAT] 06.04 protein targeting, sorting and translocation [Ξ. cerevisiae, YDR432w] 3e-04 [FUNCAT] 08.01 nuclear transport [Ξ. cerevisiae, YDR432w] 3e-04 [BLOCKS] BL00030B Eukaryotic RNA-binding region RNP-1 proteins [BLOCKS] BL00900D Bacteriophage-type RNA polymerase family proteins signatur [SCOP] dlsxl 4.34.7.1.3 Sex-lethal protein [ (Drosophila melanogaster) 9e-23 [SCOP] d2ula 4.34.7.1.2 UlA protein [human (Homo sapiens) 6e-24 [SCOP] dlupl_2 4.34.7.1.1 Nuclear ribonucleoprotein Al, RNP Al, UP le-13 [PIRKW] nucleus le-110 [PIRKW] duplication le-110 [PIRKW] RNA binding le-110 [PIRKW] nucleolus 4e-10 [PIRKW] tandem repeat 4e-10 [PIRKW] single-stranded DNA binding le-06 [PIRKW] DNA binding 9e-12 [PIRKW] phosphoprotein 4e-10 [PIRKW] mitochondrion 6e-07 [PIRKW] heterotrimer 4e-06 [PIRKW] alternative splicing le-15 [PIRKW] chloroplast 5e-ll [PIRKW] transcription regulation 3e-09 [PIRKW] GTP binding 2e-06 [SUPFAM] helix-destabilizing protein le-07 [SUPFAM] nucleolin 4e-10 [SUPFAM] glycine-rich RNA-bind g protein 2e-07 [SUPFAM] yeast HRP1 protein 2e-08 [SUPFAM] unassigned ribonucleoprotein repeat-containmg proteins 3e-25 [SUPFAM] polyadenylate-b ndmg protein le-112 [SUPFAM] ribonucleoprotein repeat homology le-112 [PROSITE] RNP_1 1 [PFAM] RNA recognition motif, (aka RRM, RBD, or RNP domain) [KW] All_Beta [KW] 3D SEQ ERSRLVCLRAAVPRMNPSTPSYPTASLYVGDLHPDVTEAMLYEKFSPAGPILSIRICRDL lhal- EEEETTTTTTCHHHHHHHHGGGCCEEEEEEEETT SEQ ITSGSSNYAYVNFQHTKDAEHALDTMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIFVKNL lhal- TTTCEEEEEEEEECCHHHHHHHHHHTTEEE-TT EEEEEEECTTTTCCCCCEEEEECC SEQ DKSINNKALYDTVSAFGNILSCNVVCDENGSKGYGFVHFETHEAAERAIKKMNGMLLNGR lhal- TTTTCHHHHHHHHGGGCCEEEEEEEETTTTTCEEEEEEECCHHHHHHHH SEQ KVFVGQFKSRKEREAELGARAKEFPNVYIKNFGEDMDDERLKDLFGKFGPALSVN lhal- Prosite for DKFZphtes3_8mlO .3 PS00030 152->160 RNP_1 PDOC00030 Pfam for DKFZphtes3_8mlO .3 HMM_NAME RNA recognition motif, (aka RRM, RBD, or RNP domain) HMM *IYVGNLPWDtTEEDLrDlFsQFGpIvsIrMMrDReTGRSRGFAFVEFED +YVG+L +D+TE +L + FS+ GPI+SIR+ RD T S +A+V+F+ Query 27 LYVGDLHPDVTEAMLYEKFSPAGPILSIRICRDLITSGSSNYAYVNFQH 75 HMM EEDAekAIdeMNGmeFmGRrlRV* DAE A+D+MN ++ G+++R+ Query 76 TKDAEHALDTMNFDVIKGKPVRI 98 HMM *IYVGNLPWDtTEEDLrDlFsQFGpIvsIrMMrDReTGRSRGFAFVEFED I+V+NL+ +++ L D S FG I+S++++ D + S+G++FV FE+ Query 115 IFVKNLDKSINNKALYDTVSAFGNILSCNVVCD—ENGSKGYGFVHFET 161 HMM EEDAekAIdeMNGmeFmGRrlRV* +E+AE+AI +MNGM+++GR++ V Query 162 HEAAERAIKKMNGMLLNGRKVFV 184 DKFZphtes3_8p7 group: testes derived DKFZphtes3_8p7 encodes a novel 412 amino acid protein without similarity to known proteins. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . unknown 2 EST hits (both from testis librarys) Sequenced by MediGenomix Locus: unknown Insert length: 2899 bp Poly A stretch at pos. 2870, polyadenylation signal at pos. 2852 1 CCGACCCGCC CTGGGGTGCT GCGTGCGCTG CCTGCTCCCG CCTGAGGAAA 51 ACACTGCCCA TGGCGCAAGG CCGGGAGCGC GACGAAGGCC CCCACTCCGC 101 CGGCGGCGCG TCCTTGTCCG TGAGATGGGT GCAAGGATTC CCTAAGCAGA 151 ATGTTCATTT GTCAACGACA ACACCATTTG CTACCCTTGT GGGAATTATG 201 TAATATTTAT TAATATTGAA ACCAAGAAAA AGACTGTACT GCAGTGTAGT 251 AATGGAATTG TGGGCGTCAT GGCAACTAAC ATCCCCTGTG AAGTTGTGGC 301 TTTTTCTGAC CGGAAGCTAA AACCTCTCAT CTACGTATAC AGCTTTCCAG 351 GATTGACCAG AAGGACCAAA TTGAAAGGCA ACATTCTCCT GGACTACACT 401 TTACTTTCAT TCAGTTACTG TGGCACCTAC CTGGCTAGTT ACTCCTCTCT 451 CCCAGAATTT GAACTGGCCC TTTGGAACTG GGAATCGAGT ATCATTTTGT 501 GTAAGAAATC ACAGCCTGGA ATGGATGTGA ACCAAATGTC TTTTAACCCC 551 ATGAACTGGC GCCAGCTGTG CTTATCAAGT CCAAGTACAG TGAGCGTGTG 601 GACCATTGAA AGAAGTAACC AGGAGCATTG TTTCAGAGCA AGGTCGGTGA 651 AATTACCTCT AGAAGATGGG TCATTTTTTA ATGAAACGGA TGTCGTTTTC 701 CCCCAGTCGT TGCCGAAAGA TCTCATCTAT GGTCCCGTGC TGCCACTGTC 751 AGCCATTGCC GGGCTGGTAG GCAAAGAGGC AGAGACTTTC CGGCCGAAAG 801 ATGATCTATA TCCTTTGCTT CACCCGACTA TGCATTGCTG GACTCCAACA 851 AGTGACTTGT ACATTGGCTG TGAAGAGGGT CATCTTTTAA TGATTAATGG 901 AGACACCTTG CAAGTGACTG TACTTAATAA GATAGAAGAG GAATCGCCAT 951 TGGAAGACAG AAGAAATTTT ATCAGTCCAG TAACCTTGGT ATATCAGAAG 1001 GAGGGCGTGC TGGCTTCTGG AATTGATGGC TTTGTGTATT CTTTTATTAT 1051 TAAAGATAGA AGTTACATGA TCGAGGATTT TCTTGAGATT GAAAGACCTG 1101 TAGAACATAT GACATTTTCT CCCAATTATA CAGTGTTGCT GATTCAAACA 1151 GACAAGGGAT CTGTTTATAT CTACACTTTT GGTAAGGAGC CAACCTTAAA 1201 TAAAGTCCTA GATGCTTGTG ATGGGAAATT TCAGGCAATT GACTTTATCA 1251 CACCTGGAAC CCAATACTTC ATGACACTTA CATATTCAGG GGAAATTTGT 1301 GTTTGGTGGC TGGAGGATTG TGCTTGTGTA AGCAAGATTT ATCTGAATAC 1351 CCTAGCAACG GTTCTGGCTT GCTGTCCATC CTCCCTCTCT GCAGCCGTGG 1401 GCACGGAGGA TGGCTCGGTC TACTTCATCA GCGTATATGA TAAGGAATCC 1451 CCTCAGGTCG TGCACAAGGC CTTTCTCTCG GAATCGTCCG TGCAGCACGT 1501 CGTGTAAGTC CTTTCTGCCT CCAGGAGCGG CTCCGTGTCA CACCCGTCTG 1551 TTGAAAATTC TAGTGAAGCC ATCCTTTCTT TTAATTTTAA GTTTTACGTG 1601 TTTCATTTGT TTTGAATGTT AATATATTCA CACAGTTCAA CACTCAAAAG 1651 GTACAGAGGG CTGTGTAGTA AAGTACCCCC CATACCCAGG TCTGTCCTTG 1701 CAGGCAGCCT GGTACCAATT TCTCATGTCT CTCCTGAGAT GTTTTATCCA 1751 TGAACAAGCA AAACATAATA AGCACTTCTT TTTACTTGTA TCAATGGCCA 1801 TCATGTGTGT ATAGTGTGCC AGGCACTTCT GCTGTATTAA CTCCATGAGG 1851 TAAACACTCT TGTTGTCTCT ATTTGACAGG TGAGGAAGAT AAGGCACAAG 1901 GATTTTAAAT AACTTGCTCA ATAGTACACA GATAGTGAAT GGCAAATGTT 1951 GGGATTTGAA CCCAGGTAGT TGGGCTGCAG AGTCACTGCC TTTGCTCTTA 2001 AAAGGAGAAA ACTATGTACA ATGCCTCATT TCTTTTTTCA CTTAATCGTA 2051 TATCTTGGAG AATGTTTTAT ATCCACACAT AAAGACCAGC CTGATTATTT 2101 GTATAGCCAC ATAGTATTCC ATTATATGAA TATACTATCA TTTTTTAAAA 2151 ACGGTATATT AATGAACATT TAGAGTATTT CAAAACTTTT GAAGCAATAC 2201 TTTTAAGATG ATAATATAGA GACATTAGAT TTGGACTTGT AGGTGCTATC 2251 ATTATTACTG TTTCTTTTTA ATTTATTATA TTATTAGGTA TTAATAAGAA 2301 CAGACATTTG TATTCTGCTT TACAGCTTGA GATCACTGTA GCTTGTGGCA 2351 TGTGATCCTC AAAACACCAG TCAGAAAGGT GTTATTCTTA TCCCTATTAG 2401 ACAAATTAGG GAATTCAGGG TTAGAGAGGT GAGGAAAAGC ATTGTCCAAG 2451 ATTACACATT ACACAGCTAG CACACTGAGG AGCTGGCCCT GCCACTGTGG 2501 ACTGCCCAGC TCCACCACCC TAGCTCAGTG GGGAAGGATG GATAACCTCC 2551 TTCCATTTAC CCCCTGCCTT TCTGCACTGT CATTTTTTTG TGCCTTTCCT 2601 TTCTCAGATC CTCTTATTCT AATTTACATC TTCCCACTTT TTCTAATTTG 2651 ATAAAGTTGT AGACATGTTT CACTACATTC TTCCTCCCAC TGCCAGGTAC 2701 CAGACACAGG GTAATGAAAT GTCACACCCA CCACTAATTT GAGAATTGCT 2751 TATTTGCGCT TGAAACATCA AGAAAGCTCT ACCGACAGAC ATGTTTCATT 2801 CACTTATGAT GAACCAACTG CCCATCTTTA CTGAATCTTC TTGACTGTAT 2851 TTATTAAAGT TGCAATTTGG AAATAAAAAA AAAAAAAAAA AAAAAAAGG BLAST Results No BLAST result Medline entries No Medlme entry Peptide information for frame 2 ORF from 269 bp to 1504 bp; peptide length: 412 Category: putative protein Classification: no clue 1 MATNIPCEVV AFSDRKLKPL IYVYSFPGLT RRTKLKGNIL LDYTLLSFSY 51 CGTYLASYSS LPEFELALWN WESSIILCKK SQPGMDVNQM SFNPMNWRQL 101 CLSSPSTVSV WTIERSNQEH CFRARSVKLP LEDGSFFNET DVVFPQSLPK 151 DLIYGPVLPL SAIAGLVGKE AETFRPKDDL YPLLHPTMHC WTPTSDLYIG 201 CEEGHLLMIN GDTLQVTVLN KIEEESPLED RRNFISPVTL VYQKEGVLAS 251 GIDGFVYSFI IKDRSYMIED FLEIERPVEH MTFSPNYTVL LIQTDKGSVY 301 IYTFGKEPTL NKVLDACDGK FQAIDFITPG TQYFMTLTYΞ GEICVWWLED 351 CACVSKIYLN TLATVLACCP SSLSAAVGTE DGSVYFISVY DKESPQVVHK 401 AFLSESSVQH VV BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_8p7, frame 2 No Alert BLASTP hits found Pedant information for DKFZphtes3_8p7, frame 2 Report for DKFZphtes3_8p7.2 [LENGTH] 412 [MW] 46476.62 [pi] 4.91 [KW] Alpha_Beta SEQ MATNIPCEVVAFSDRKLKPLIYVYSFPGLTRRTKLKGNILLDYTLLSFSYCGTYLASYSS PRD cccccceeeeeecccccceeeeeecccccccccccchhhhhhhheeeecccccccccccc SEQ LPEFELALWNWESΞIILCKKSQPGMDVNQMSFNPMNWRQLCLSSPSTVSVWTIERSNQEH PRD cchhhhhhhhccccceeeccccccccceeeccccccceeeeeccccceeeeeeeecchhh SEQ CFRARSVKLPLEDGSFFNETDVVFPQSLPKDLIYGPVLPLSAIAGLVGKEAETFRPKDDL PRD hhhhhhhcccccccccccccccccccccccccccccccceeeeeeccccccccccccccc SEQ YPLLHPTMHCWTPTSDLYIGCEEGHLLMINGDTLQVTVLNKIEEESPLEDRRNFISPVTL PRD cccccccccccccccceeeecccceeeecccceeeeeehhhhhcccccccccccccccee SEQ VYQKEGVLASGIDGFVYSFIIKDRSYMIEDFLEIERPVEHMTFSPNYTVLLIQTDKGSVY PRD eeeceeeeecccceeeeeeeeeccchhhhhhhhhhcccceeeccccceeeeeecccccee SEQ IYTFGKEPTLNKVLDACDGKFQAIDFITPGTQYFMTLTYSGEICVWWLEDCACVSKIYLN PRD eeeccccccchhhhhcccccceeeeeccccceeeeeeeccceeeeeeecceeeeeeeehh SEQ TLATVLACCPSΞLSAAVGTEDGSVYFIΞVYDKESPQVVHKAFLSESSVQHVV PRD hhhhhhhccccccceeeeccccceeeeeeeccccccchhhhhhhcccccccc (No Prosite data available for DKFZphtes3_8p7.2) (No Pfam data available for DKFZphtes3_8p7.2) DKFZphtes3_9e22 group: testes derived DKFZphtes3_9e22 encodes a novel 227 amino acid protein with weak partial similarity to Ring- finger proteins. For the novel protein, Pfam, but not Prosite predicts a C3HC4 type RING fmger motife. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application m studying the expression profile of testis-specific genes . similarity to zmc finger proteins Sequenced by DKFZ Locus : unknown Insert length: 1318 bp Poly A stretch at pos. 1308, no polyadenylation signal found 1 GCTCCCCCGG CTTTCGGAGC CCGGGGGCGG CCTGTGGCGC GCGGAGCCCG 51 CGCCGGACTG CGCCTCTTTG GACCTTGAGG GGAAACATGC GTTTGCCTTG 101 GATCGTTTGA AATTCTAAGT TTGGGATCCC CGCCCGCCCG CCTGCCTCTT 151 CCGCCCCGCG GGTTTTTTCC TTTTTTCCTT TTGCTTTTTT TCCTTTTCTC 201 CCTCCGGGTC TCCTTTTTGA CTCCCTCCCC CTTTATGCTC GCCCAGCCCT 251 CCCCCTGCTG CTGAGAAGTG GGGGAGGGTC TCGGCCTCCA GGTTCCCGCC 301 CCACCGGGGC CCGGGCGAGC ATGGGGGGCA AGCAGAGCAC GGCGGCCCGC 351 TCCCGGGGCC CCTTCCCGGG GGTCTCCACC GATGACAGCG CCGTGCCGCC 401 GCCGGGAGGG GCGCCCCATT TCGGGCACTA CCGGACGGGC GGCGGGGCCA 451 TGGGGCTGCG CAGCCGCTCG GTCAGCTCGG TGGCAGGCAT GGGCATGGAC 501 CCCAGCACGG CCGGGGGGGT GCCCTTTGGC CTCTACACCC CCGCCTCCCG 551 GGGCACCGGC GACTCCGAGA GGGCGCCCGG CGGCGGAGGG TCTGCGTCCG 601 ACTCCACCTA TGCCCATGGC AATGGTTACC AGGAGACGGG CGGCGGTCAC 651 CATAGAGACG GGATGCTGTA CCTGGGCTCC CGAGCCTCGC TGGCGGATGC 701 TCTACCTCTG CACATCGCAC CCAGGTGGTT CAGCTCGCAT AGTGGTTTCA 751 AGTGCCCCAT TTGCTCCAAG TCTGTGGCTT CTGACGAGAT GGAAATGCAC 801 TTTATAATGT GTTTGAGCAA ACCTCGCCTC TCCTACAACG ATGATGTGCT 851 GACTAAAGAC GCGGGTGAGT GTGTGATCTG CCTGGAGGAG CTGCTGCAGG 901 GGGACACGAT AGCCAGGCTG CCCTGCCTGT GCATCTATCA CAAAAGCTGC 951 ATAGACTCGT GGTTTGAAGT GAACAGATCT TGTCCGGAAC ACCCTGCGGA 1001 CTGACCTGCG GGCTTGCTTG CTGACTCCTC TCAAAGGGAC AGAGCGCCCC 1051 TGCTCCAGGG AGGAGGCTCA CCGGACCCTG GGGCAGAGCT GAGCTTGGGA 1101 CACCAGCGGG AACAGGGCAC CCCTTCTGCA CTGACTTCCA GATCATGGTT 1151 CTCCCTTCCT CCCTGAGGAC ACCAAATTGG ATGAGAGCAA GTTTGAGAGA 1201 AGAATGAATC AACTGCTATC CTTCCCCTCA CCCCTCAGCC CAGGAGGGAA 1251 AGGGCATTTT CTTTTTCATC TTTGAAAGGC ATTGTGGGTC TGTCTTTAAA 1301 GTGTTTACAA AAAAAAAA BLAST Results No BLAST result Medlme entries No Medlme entry Peptide information for frame 3 ORF from 321 bp to 1001 bp; peptide length: 227 Category: similarity to known protein Classification: unclassified 1 MGGKQSTAAR SRGPFPGVST DDSAVPPPGG APHFGHYRTG GGAMGLRSRS 51 VSSVAGMGMD PSTAGGVPFG LYTPASRGTG DSERAPGGGG SASDSTYAHG 101 NGYQETGGGH HRDGMLYLGS RASLADALPL HIAPRWFSSH SGFKCPICSK 151 SVASDEMEMH FIMCLSKPRL SYNDDVLTKD AGECVICLEE LLQGDTIARL 201 PCLCIYHKSC IDSWFEVNRS CPEHPAD BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_9e22, frame 3 TREMBL:AF078823_1 product: "RING-H2 finger protein RHA2b"; Arabidopsis thaliana RING-H2 fmger protein RHA2b mRNA, complete eds., N = 1, Score = 111, P = 2.8e-06 TREMBL:AF078822_1 product: "RING-H2 finger protein RHA2a"; Arabidopsis thaliana RING-H2 fmger protein RHA2a mRNA, complete eds., N = 1, Score = 112, P = 6.6e-06 TREMBL:AC004138_14 gene: "T17M13.17"; Arabidopsis thaliana chromosome II BAC T17M13 genomic sequence, complete sequence., N = 2, Score = 123, P = 1.4e-05 PIR:T02286 hypothetical protein T13D8.23 - Arabidopsis thaliana, N = 1, Score = 142, P = 8.8e-08 >PIR:T02286 hypothetical protein T13D8.23 - Arabidopsis thaliana Length = 327 HSPs: Score = 142 (21.3 bits), Expect = 8.8e-08, P = 8.8e-08 Identities = 24/57 (42%), Positives = 30/57 (52%) Query: 166 SKPRLSYNDDVLTKDAGECVICLEELLQGDTIARLPCLCIYHKSCIDSWFEVNRSCP 222 S P + LT D +C +C+EE + G LPC IYHK CI W +N SCP Sbjct: 206 SLPSVKITPQHLTNDMSQCTVCMEEFIVGGDATELPCKHIYHKDCIVPWLRLNNSCP 262 Pedant information for DKFZphtes3_9e22, frame 3 Report for DKFZphtes3_9e22.3 [LENGTH] 227 [MW] 23782.62 [pi] 6.18 [HOMOL] PIR:T02286 hypothetical protein T13D8.23 - Arabidopsis thaliana 2e-08 [FUNCAT] 99 unclassified proteins [S. cerevisiae, YDR313c] 4e-06 [FUNCAT] 30.07 organization of endoplasmatic reticulum [S. cerevisiae, YOL013c] 0.001 [FUNCAT] 06.13 proteolysis [S. cerevisiae, YOL013c] 0.001 [PFAM] Zmc finger, C3HC4 type (RING finger) [KW] Irregular SEQ MGGKQSTAARSRGPFPGVSTDDSAVPPPGGAPHFGHYRTGGGAMGLRSRSVSSVAGMGMD PRD cccccccccccccccccccccccccccccccccccccccccccccccccceeeccccccc SEQ PSTAGGVPFGLYTPASRGTGDSERAPGGGGSASDSTYAHGNGYQETGGGHHRDGMLYLGS PRD ccccccccccccccccccccccccccccccccccccccccccccccccccccccceeech SEQ RASLADALPLHIAPRWFΞSHSGFKCPICSKSVASDEMEMHFIMCLSKPRLSYNDDVLTKD PRD hhhhhhhhceeecccccccccccccccccccchhhhhhhhhhhhcccccccccccccccc SEQ AGECVICLEELLQGDTIARLPCLCIYHKSCIDSWFEVNRSCPEHPAD PRD cceeeeeecccccccccccccceeeeeeccchhhhhhhhcccccccc (No Prosite data available for DKFZphtes3_9e22.3) Pfam for DKFZphtes3_9e22.3 HMM_NAME Zmc fmger, C3HC4 type (RING finger) HMM *CPICFcTFQlDyPWPFdePmMlPCgHsFCypCIrrW CPmC* C IC L+++ D++ LPC+ ++ ++CI +W CP+ Query 184 CVIC LEELLQGDTIARLPCLCIYHKSCIDSWFEVNRSCPEH 224 DKFZphtes3_9ι20 group: testes derived DKFZphtes3_9ι20 encodes a novel 205 ammo acid protein with similarity to human KIAA0336 gene. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application in studying the expression profile of testis-specific genes . unknown complete cDNA, complete eds, EST hits Sequenced by DKFZ Locus: /map="44.1 cR from top of Chrl7 linkage group" Insert length: 2509 bp Poly A stretch at pos. 2499, polyadenylation signal at pos. 2481 1 CTCGCCGAGA TGACCTGGGC ACCTCTGCGT TGAATCGGCA AATACTGATC 51 AAGCCGCATT TATTCTGCTC TCAGGAACTC TAAGTCTAGC AGAGAAGATG 101 AGGCGGTAGA AGTTCATCAA TGGCTTGGCT GGAGGACAAG CAAATTGAGG 151 ACATTGGCAA CGGAGTGATC AAAATGATAG ATCATGAGGC CTAAAATGAA 201 TAAGGAAAGA AGAGAAGTGG CAGAGGCTGA GAACAGAAAG AGAGGGTGGA 251 GGGGCTGTAA ATCTTGAAGA TTAGGGTATA ATATGAGTAT ATGGGTAAGA 301 ATTGGAAGAA TTGTGTAGGA GGCAGTAGTC AAAAAGTAGA AGCAGTTTGG 351 AAGAGTAGTT ACAAATATCA AGAGCCAGGT GGCTAAAAGG TGGAGCTATA 401 GGTCATTGAA GCTCAAGAAA CTGAGTCTCT AGGGCATTGG TTAAGTCATC 451 TGTCTAGACT TCAAAGTTGT CTAGGATGAT AATTCAGAAG ACTGATCTGT 501 GCCAAAGTCA CAGGTTTTTC ACGACTGAAA ACAACATAGC AAAATAAGCC 551 AAGATGTCTG TGGATCCAAT GACCTACGAG GCCCAGTTCT TTGGCTTCAC 601 GCCACAAACG TGCATGCTTC GGATCTACAT TGCATTTCAA GACTACCTAT 651 TTGAAGTGAT GCAGGCCGTT GAACAGGTTA TTCTGAAGAA GCTGGATGGC 701 ATCCCAGACT GTGACATTAG CCCAGTGCAG ATTCGCAAAT GCACAGAGAA 751 GTTTCTTTGC TTCATGAAAG GACATTTTGA TAACCTTTTT AGCAAAATGG 801 AGCAACTGTT TTTGCAGCTG ATTTTACGTA TTCCCTCAAA CATCTTGCTT 851 CCTGAAGATA AATGTAAGGA GACACCTTAT AGTGAGGAAG ATTTTCAGCA 901 TCTCCAGAAA GAAATTGAAC AGTTACAGGA GAAGTACAAG ACTGAATTAT 951 GTACTAAGCA GGCCCTTCTT GCAGAATTAG AAGAGCAAAA AATTGTTCAG 1001 GCCAAACTCA AACAGACGTT GACTTTCTTT GATGAGCTTC ATAATGTTGG 1051 CAGAGATCAT GGGACTAGTG ATTTTAGGGA GAGTTTAGTA TCCCTGGTTC 1101 AGAACTCCAG AAAACTACAG AACATTAGAG ACAATGTGGA AAAGGAATCG 1151 AAACGACTGA AAATATCTTA ATTGCTCAGT AGTCAAAAGG AGGAGCCTGT 1201 CAAAAAGTAG AATCATAAGG ACTGTTCAAA CCATAAGGAC TGTTCAAATC 1251 ATACCAGTGA CTGTTCAAAC CAACCATACT TTTTATTAGA TTTGCTTTGT 1301 CAACTCTTTC TTGTATTCTG TGTTTTCCTC TTTTTTGGTC CACTTTGCTG 1351 AGGTATGAAG TGTACTACTT TGAACTAGGC TGAAGCATCT GAGTCTTCTA 1401 ATAAGTGGGA AGGGATCCAA CAAAGAAGCC ATGACCAGTT AAAGATATTT 1451 GCAGAGTTAC ACCTTGGTCA TAAGTCCTTT GTGACCTTGA TTATTTTGGC 1501 TTACTCTTTG GATGAGACCA GACAAGAAAA GGATTAAACG GGTGGCTCCT 1551 TTAATATTAT TATTATTGTT TTTGAGACAA GGTCCCTTTC TGTCACCCAG 1601 GTTAGAGTAG ATTTCAGTGG CACAATCTTG GCTCACTGCA ACCTCTGTGT 1651 CCTGGGCTCA AGTGATCCTC CTGCCTCAGC CTCCCAAGTA GCTAGGACCA 1701 CAGGTGCGTG TCACCATGCT TGGCTAATTT TTTTGCAGAA ACGAGGCCTC 1751 ACTATATTGT CCAGGCTGAG TGGCTCTTTT ATTAACCAGT CATTACACTG 1801 CGGAACAGCC AACATAGAGT ACTTGCTCTC GTCCTGTGAA TTTTCTTTCA 1851 TGAGGGAGTC AATATGTAGT GGAAAGAAGC ATGTAGCAAA AAAGACAACC 1901 TTGATCTTTA ATAAAAAAGA AGTTGGTTTA TTTCCAAAAT AAATCCCCTG 1951 ACAAAAAACC TGGTGATGTT AAGCAATTGA CTGTCTTAGA GTCCAGCAGA 2001 AGACCTTAGA CAAAAAAAGC AGAACCCACT GGAGTAGAAA AGGAAGCATG 2051 TAGCATATAC TCAGTAGTGA AATTTAATTT TACTGACTGT TAGGTATCTA 2101 TGCCAATTTG TTTTCATACT TCAGTTGGTT TTGGAATCTG CCTTATACCT 2151 AATATTTATT TATTCACACT CATAAGCATC AAATATTTAA TGCCCTCAGT 2201 GGGAAATTTG TGTTTAAACT CAATGGAATC TAATATTTCT TTATGTCGTT 2251 AGTCCCTGTA AAATGTTAGG TCACCCAAGG AAAGGGGAGA AATAGCAATG 2301 GTTGTTCCTA AGGTATTGCT TGCCCTCCAT GTCTTCCTAA AGAGCAGAAC 2351 TTGGAGTTTC TCCTTTATGT AGAGAAGAAG TAACTTAGGG TGTATTTGCA 2401 ATGAAATATT CATAGATATT GAAAGCTTGT GTTTACATGA AATATGTTTA 2451 TTATCAAGAA GTCCTTTTTC CAATTCTGTA CATTAAATAT ATGTGTTTTA 2501 AAAAAAAAA BLAST Results Entry AC004148 from database EMBL: Homo sapiens chromosome 17, clone HCIT524C5, complete sequence. Score = 5245, P = O.Oe+00, identities = 1049/1049 3 exons Entry HS556361 from database EMBL: human STS TIGR-A003N29. Score = 1005, P = 1.3e-39, identities = 201/201 Entry HSG043 from database EMBL: human STS SHGC-36031. Score = 955, P = 2.8e-37, identities = 205/215 Medlme entries No Medline entry Peptide information for frame 2 ORF from 554 bp to 1168 bp; peptide length: 205 Category: putative protein Classification: no clue 1 MSVDPMTYEA QFFGFTPQTC MLRIYIAFQD YLFEVMQAVE QVILKKLDGI 51 PDCDISPVQI RKCTEKFLCF MKGHFDNLFS KMEQLFLQLI LRIPSNILLP 101 EDKCKETPYS EEDFQHLQKE IEQLQEKYKT ELCTKQALLA ELEEQKIVQA 151 KLKQTLTFFD ELHNVGRDHG TSDFRESLVS LVQNSRKLQN IRDNVEKESK 201 RLKIS BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_9ι20, frame 2 TREMBLNE :HSAB2334_1 gene: "KIAA0336"; Human mRNA for KIAA0336 gene, complete eds., N = 1, Score = 107, P = 0.0081 >TREMBLNEW:HSAB2334_1 gene: "KIAA0336"; Human mRNA for KIAA0336 gene, complete eds. Length = 1,583 HSPs: Score = 107 (16.1 bits), Expect = 8.2e-03, P = 8.1e-03 Identities = 42/140 (30%), Positives = 76/140 (54%) Query: 65 EKFLCFMKGHFDNLFSKMEQLFLQLILRIPSNILLPEDKCKETPYSEED FQHLQKE 120 EK CF+K H +NL +EQ +L R ILL +D ++P + D + L+++ Sbjct: 796 EKEKCFIKEH-ENLKPLLEQK—ELRDRRAELILL-KDSLAKSPSVKNDPLSSVKELEEK 851 Query: 121 IEQLQE—KYKTELCTKQALLAELEEQKIVQAKLKQTLTFFDELHNVGRDHGTSDFRESL 178 IE L++ K K E K L+A ++ +K + + K+T T +EL ++ + S+ Sbjct: 852 IENLEKECKEKEEKINKIKLVA-VKAKKELDSSRKETQTVKEELESLRSEK—DQLSASM 908 Query: 179 VSLVQNSRKLQNIRDNVEKESKRLKI 204 L+Q + +N+ EK+S++L + Sbjct: 909 RDLIQGAESYKNLLLEYEKQSEQLDV 934 Pedant information for DKFZphtes3_9ι20, frame 2 Report for DKFZphtes3_9ι20.2 [LENGTH] 205 [MW] 24140.13 [pi] 5.51 [KW] All_Alpha [KW] COILED_COIL 18.05 % SEQ MSVDPMTYEAQFFGFTPQTCMLRIYIAFQDYLFEVMQAVEQVILKKLDGIPDCDISPVQI PRD cccccchhhhhhcccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhccccccccccccc COILS SEQ RKCTEKFLCFMKGHFDNLFSKMEQLFLQLILRIPSNILLPEDKCKETPYSEEDFQHLQKE PRD cccchhhhhhhcccccchhhhhhhhhhhhhhhcccceeeccccccccccchhhhhhhhhh COILS CCCCCCCCCC SEQ IEQLQEKYKTELCTKQALLAELEEQKIVQAKLKQTLTFFDELHNVGRDHGTSDFRESLVS PRD hhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhcccccccccccchhhhhhhh COILS CCCCCCCCCCCCCCCCCCCCCCCCCCC SEQ LVQNSRKLQNIRDNVEKESKRLKIS PRD hhcccchhhhhhhhhhhhhhhcccc COILS (No Prosite data available for DKFZphtes3_9 20.2) (No Pfam data available for DKFZphtes3 9ι20.2) DKFZphtes3_9k22 group: testes derived DKFZphtes3_9k22 encodes a novel 304 ammo acid protein with partial similarity to X. leavis katanin p80. No informative BLAST results; No predictive prosite, pfam or SCOP motife. The new protein can find application m studying the expression profile of testis-specific genes . similarity to C-terminus of katanin p80 Sequenced by DKFZ Locus : unknown Insert length: 2676 bp Poly A stretch at pos. 2665, no polyadenylation signal found 1 CTCTCTAGGC TGCCGGGCGC TGGTCGTCAG CGCCGAGGCT GGGCTGAGGC 51 GCCGCGGTAC CATGAGGCGC CGGTACTTAA GAGATTATGG CATCAGAAAC 101 CCACAATGTT AAAAAACGGA ACTTTTGTAA TAAGATTGAG GATCATTTCA 151 TTGATCTTCC TAGAAAAAAG ATCTCTAATT TCACTAATAA GAACATGAAG 201 GAGGTTAAGA AATCTCCAAA ACAGTTGGCT GCTTACATAA ATAGAACAGT 251 TGGACAAACT GTGAAAAGCC CAGATAAACT TCGTAAAGTG ATCTATCGCA 301 GAAAGAAAGT TCATCATCCC TTTCCAAATC CTTGTTACAG AAAAAAACAG 351 TCCCCTGGAA GTGGGGGCTG TGACATGGCA AATAAAGAAA ATGAACTGGC 401 TTGTGCAGGC CACCTGCCTG AAAAATTACA CCATGATAGT CGAACATATT 451 TGGTTAACTC CAGTGATTCT GGTTCTTCAC AGACAGAAAG CCCATCATCA 501 AAATATAGTG GGTTTTTTTC TGAGGTTTCT CAGGACCATG AAACAATGGC 551 CCAAGTTTTG TTCAGCAGGA ATATGAGATT GAATGTAGCT TTAACTTTCT 601 GGAGAAAGAG AAGTATAAGT GAACTTGTAG CTTATTTGTT GAGGATAGAA 651 GATCTTGGCG TTGTGGTAGA TTGCCTTCCT GTGCTCACCA ATTGTTTACA 701 GGAAGAAAAA CAATATATCT CACTTGGCTG CTGTGTTGAC TTGTTGCCTC 751 TAGTAAAGTC ACTACTTAAA AGCAAATTTG AAGAATATGT TATAGTTGGT 801 TTAAACTGGC TTCAAGCAGT CATTAAAAGG TGGTGGTCAG AACTATCATC 851 CAAAACAGAA ATTATAAATG ATGGAAATAT TCAAATTTTA AAACAACAAT 901 TAAGTGGATT ATGGGAACAG GAAAACCATC TTACTTTGGT TCCAGGATAT 951 ACTGGTAATA TAGCTAAGGA TGTAGATGCT TATTTATTAC AGTTACATTG 1001 AGAGATTTCA TCTACTAAAG AGCATTTGGT TTTTCAAAAC ATCCCTGAAC 1051 TGTATAATTT ACAAAAAAAA AAGTCTCGTC TGAGAACTGT GAACTGTGGA 1101 AGAAATCAAA ACTATTTTTT CTTTTAAAAA GCCACGTAAT GAAACCACTA 1151 ATGAAATCCC AGCAATCTGC TTCACATTGA AGTGGAAAAA TATCCAAAAG 1201 GAGCAGCTTC AATTTCATTG AGGTGAAAGT GCACTATGAA GATTGTTCAC 1251 CTTTGCTGCA TTTGGGAGTT ATATGGTTAT TTGGTAACAT TAAGAACTAC 1301 TGGATTTTAA TGCAATCCTG CATAAAAATA TAATTTATAC TATGTGAAAA 1351 AATAAGACAG GACTTACCAC TAGGAACCAC CAAGACCAAT CATCATTAAC 1401 TTTTTTAAGA TTGTGTTTTA TTAAAAAAAA AAAACACTTA AATGTGTGCA 1451 GCTATTTTCT TATGTTGAAA AGACTGAAAG TTTAAAACAT GAAAAAAATC 1501 AATATTAAAC ATTTTTTGTT CACACTGAGA TACTGTGTAT GTAAAATGCC 1551 TTAATTATTA ATAAGCCAAT GTGTTATGAT ACCAATATCT GTTTTAAAAA 1601 ACTAAAACCA ACCATGCTTC TGGCATGATA AAATCATGGA ATTAAATCAG 1651 GGGTTTACAT TCTTGTAGAG TGTTCTTGAA ACACTCTCTG CACCATTTTT 1701 AAAACTTGAG AATAGTTTTA GTATCTCTGA TATTTTTTGC CAGAATCATC 1751 ATGTCATGTA TGAATGTGTT ATCCCTATCT AAGGAAAAAG GTGAATATGT 1801 TTTTGTATGA ATGTTTAACT GGAAATGTCC ATGGACTTGG CTAATTTATA 1851 TTTACTTTTT ATTGTACATA GATTTCTAAT ATTTTTCATT CCTGTATCAT 1901 TTAAACTTCC TTCATTTGAG TAAATTCACT AAATATTTCT ATTTTTTTGC 1951 TTTTTTAAAT TCTGATTTTA TATGAATTCT AATTCTTTTT CACTACATAT 2001 GTTTTAAAGA GTTACATACA GTGATTTAGA ATGGTTTACA GTTAATGCTG 2051 ATCTTGTATT TTAAATTCCA ACACTTTGTG TCACTACCTC CTCTAATGGT 2101 TAGTATGATA TGCTAGCAGA CTGTATGAGG TCTTTTTTTA AAATACCACT 2151 TTTAGTGTCA GTGAACCAAA TTCTGGAATG TCTTAACAGC TCTAAATCTT 2201 ACTTGTCTTG AAAATGATTG GGGTTTAATA CCACTGCTGG TGGTTCACAC 2251 ATCATCCCAT CCTTAATATG CCTGACAGGC ATCTGAGCAA AGGTTTTTAG 2301 TAATTGAATT TCTCTGCAGT AGTCCTTCAA GCACTTGAAT GTAAACCTTT 2351 AGCATTTATT CGTTTAATGA CTACTGATAC GAATCTCAAG CAGATTTCTT 2401 GCTCTTAAAA GTTATGTTTC ACTGAGTTCT GGTTTTGTGT AGCTATATTT 2451 TATATAGCTA GATATTCCTC ACAGTGAACA TGAATTGTAA TAATTGGTTA 2501 TTTCCTTAAG TCTTTAGATT ATAATAATTT CAGATTATTG CACGTCTGTG 2551 ATTTGAGAGG TGAGTTATTT AAGAGGCCAG TTTTCAGGAC ATGGGAATTT 2601 GAATTGTAAA CCTGTTATCT CTGTGAAACT TTTAACATGA TAAAATATAA 2651 CCTTTCTTTG TGCTTAAAAA AAAAAA BLAST Results Entry HS541354 from database EMBL: human STS WI-11840. Score = 1267, P = 7. le-50, identities = 271/281 Medline entries 98227670: Katanin, a microtubule-severing protein, is a novel AAA ATPase that targets to the centrosome using a WD40-contaιnιng subunit. Peptide information for frame 3 ORF from 87 bp to 998 bp; peptide length: 304 Category: similarity to known protein Classification: unclassified 1 MASETHNVKK RNFCNKIEDH FIDLPRKKIS NFTNKNMKEV KKSPKQLAAY 51 INRTVGQTVK SPDKLRKVIY RRKKVHHPFP NPCYRKKQSP GSGGCDMANK 101 ENELACAGHL PEKLHHDSRT YLVNSSDSGS ΞQTESPSSKY SGFFSEVSQD 151 HETMAQVLFS RNMRLNVALT FWRKRSISEL VAYLLRIEDL GVVVDCLPVL 201 TNCLQEEKQY ISLGCCVDLL PLVKSLLKSK FEEYVIVGLN WLQAVIKRWW 251 SELSSKTEII NDGNIQILKQ QLSGLWEQEN HLTLVPGYTG NIAKDVDAYL 301 LQLH BLASTP hits No BLASTP hits available Alert BLASTP hits for DKFZphtes3_9k22, frame 3 TREMBL: AF056021_1 product: "p80 katanin"; Xenopus laevis p80 katanin mRNA, partial eds., N = 1, Score = 146, P = 1.2e-07 TREMBL:AF052432_1 product: "katanin p80 subunit"; Homo sapiens katanin p80 subunit mRNA, complete eds., N = 1, Score = 150, P = 1.2e-07 TREMBL: AF052433_1 product: "katanin p80 subunit"; Strongylocentrotus purpuratus katanin p80 subunit mRNA, complete eds., N = 2, Score = 146, P = 4.2e-07 >TREMBL: AF052432_1 product: "katanin p80 subunit"; Homo sapiens katanin p80 subunit mRNA, complete eds. Length = 655 HSPs: Score = 150 (22.5 bits), Expect = 1.2e-07, P = 1.2e-07 Identities = 35/105 (33%), Positives = 55/105 (52%) Query: 145 SEVSQDHETMAQVLFSRNMRLNVALTFWRKRSISELVAYLLRIEDLGVVVDCLPVLTNCL 204 S++ + H+TM VL SR+ L+ W I V + I DL VVVD L N + Sb j ct: 489 SQIRKGHDTMCVVLTΞRHKNLDTVRAVWTMGDIKTSVDSAVAINDLSVVVDLL NIV 544 Query: 205 QEEKQYISLGCCVDLLPLVKSLLKSKFEEYVIVGLNWLQAVIKRW 249 ++ L C +LP ++ LL+SK+E YV G L+ +++R+ Sbjct: 545 NQKASLWKLDLCTTVLPQIEKLLQSKYESYVQTGCTSLKLILQRF 589 Pedant information for DKFZphtes3_9k22, frame 3 Report for DKFZphtes3_9k22.3 [LENGTH] 304 [MW] 34767.24 [pi] 9.18 [KW] All Alpha [KW] LOW_COMPLEXITY 3.95 % SEQ MAΞETHNVKKRNFCNKIEDHFIDLPRKKISNFTNKNMKEVKKSPKQLAAYINRTVGQTVK SEG PRD ccccccccccccccccccccccccccccccccccccccccccccchhhhhhhhhhccccc SEQ SPDKLRKVIYRRKKVHHPFPNPCYRKKQSPGSGGCDMANKENELACAGHLPEKLHHDSRT SEG PRD ccchhhhhhhhhhhcccccccccccccccccccccccccchhhhhhccccccccccccce SEQ YLVNSSDSGSSQTESPSSKYSGFFSEVSQDHETMAQVLFSRNMRLNVALTFWRKRSISEL SEG PRD eeecccccccccccccccccccccccccchhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh SEQ VAYLLRIEDLGVVVDCLPVLTNCLQEEKQYISLGCCVDLLPLVKSLLKSKFEEYVIVGLN SEG xxxxxxxxxxxx PRD hhhhhhhhhcceeeeeeccchhhhhhhhceeeccceeeehhhhhhhhhhhheeeeeeehh SEQ WLQAVIKRWWSELSSKTEIINDGNIQILKQQLSGLWEQENHLTLVPGYTGNIAKDVDAYL SEG PRD hhhhhhhhhhhhcccceeeeccccccccccccchhhhhhhhhhccccccccchhhhhhhh SEQ LQLH SEG .... PRD hccc (No Prosite data available for DKFZphtes3_9k22.3) (No Pfam data available for DKFZphtes3_9k22.3) Localization of expressed proteins KΩ KΩ KΩ KΩ 00 KΩ KΩ KΩ CD O O o o o o l\3 o o to o CD CD O cn Table of cDNA clones and related data Group: cell cycle hfbr2_16gl8 Similarity to KIAA0797 and yeast Novel protein with similarities to S. pombe SPAC17A5.07C and the S. cerevisiae Cell cycle Smt4p Smt4p suppressor of MIF2 gene.; involved in centromer organisation hfbr2 2kl4 Strong similarity to human N 33 New tumour suppressor gene Cell cycle tumour suppressor gene htes 3 3 5b4 Human M-phase phosphoprotein-1 The novel protein is C-terminal identical to human M-phase phosphoprotein-1, which Cell cycle is expressed and phosphorylated in the metaphase . Therefore the novel protein seems to be involved in the mitotic spindle during cell division. htes3_35p22 Strong similarity to oncogene 1 Oncogene Cell cycle (tre-2 locus) htes3_7j3 Related to the C-TAKl Cdc25C Cdc25C is a protein kinase that controls entry into mitosis by dephosphorylation Cell cycle associated protein kinase of Cdc2. Cdc25C function is regulated by phosphorylation, too. Serine 216 phosphorylation of Cdc25C mediates the binding of 14-3-3 protein to Cdc25C. C-TAKl (Cdc twenty- five htes3_7pl0 Strong similarity to XPMC2 protein XPMC2 of xenopus rescues several different yeast mitotic catastrophe mutants Cell cycle defective in Weel/Mikl kinase function. hutel 20mll Similarity to suppressor protein Suppressor regulator of protein phosphatase-1 Cell cycle sds22 O CO Group cell structure and motility Group Differentiation/Development hfbr2 2dl5 Mus musculus testis-specific Y- TSPY is believed to function in early spermatogenesis and is a candidate for GBY, Differentiat encoded-like protein (Tspyll) the putative gonadoblastoma-inducing gene on the Y-chromosome ion/Developm ent htes3 35e21 Similarity to interleukin-7 New interleukin Differentiat precursor ion/Developm ent hutel 2h3 Strong similarity to mouse E25 and Homolog is marker for chondro-osteogenic dif erentiation Differentiat gallus E3-16 ion/Developm ent O O Group kidney derived hfkd2_l 9 Strong similarity to XLCL2 No informative BLAST results, No predictive prosite, pfam or SCOP motive Kidney protein, African clawed frog derived hfkd2 24e23 Unknown No informative BLAST results, No predictive prosite, pfam or SCOP motive Kidney derived hfkd2 46a6 Unknown No informative BLAST results, No predictive prosite, pfam or SCOP motive Kidney derived hfkd2 46bl0 Similarity to C elegans F25B5 3 No informative BLAST results, No predictive prosite, pfam or SCOP motive Kidney derived hfkd2 46dl3 Weak similarity to KE03 protein Contains a RGD site, Kidney No informative BLAST results, No predictive prosite, pfam or SCOP motive derived hfkd2 4b6 Similarity to Homo sapiens clone No informative BLAST results. No predictive prosite, pfam or SCOP motive Kidney 25003 partial CDS. derived hfkd2 4c8 Similarity to KIAA0549 and HAP1 No informative BLAST results, No predictive prosite, pfam or SCOP motive Kidney (Huntingtin-associated protein-1) derived Group mammary carcinoma derived hmcfl 1C23 Unknown ;No informative BLAST results; No predictive prosite, pfam or SCOP motive Mammary Carcinoma derived hmcfl_lgl3 Similarity to KIAA0766; very weak No informative BLAST results; No predictive prosite, pfam or SCOP motive Mammary similarioty to transposases Carcinoma derived O Group Nucleic acid management hfbr2 23bl0 Similarity to rat RNA helicase RNA helicase Nucleic Acid HEL117 Management hfbr2 3cl8 Strong similarity to RNA helicase DEAD-box Nucleic Acid and RNA-dependent ATPase from the Management DEAD box family hfbr2 64al5 Similarity to inorganic Inorganic pyrophosphatase Nucleic Acid pyrophosphatases (unspliced) Management hfbr2 6ol7 Strong similar to RNA helicases RNA helicases Nucleic Acid Management hfbr2 72bl8 Similarity to DNA damage induced Similar to dinP of E. coli, yqjH of B. subtilis, dinP of M. tuberculosis and Nucleic Acid genes T19K24.15 of A. thaliana. The dinB/P pathway is a second SOS-pathway in E. coli Management hfbr2 72112 Similarity to YDR126W DNA binding protein Nucleic acid managment hfbr2 82i24 Strong similarity to DEAD-box Dead-box helicase Nucleic Acid subfamily ATP-dependent helicase Management htes3 14h21 Strong similarity to RNA helicases RNA helicase Nucleic Acid Management htes3_15j3 Similarity to YGR276C, a Rnase H Nucleic Acid ribonuclease H of S . cerevisiae . Management htes 3 20ml8 Similarity to the S. cerevisiae The novel protein contains a leucine zipper and a Prosite mitochondrial energy Nucleic Acid mitochondrial carrier protein transfer proteins signature. It is member of a family of substrate carrier Management RIM2. proteins which are found in the inner mitochondrial membrane and are involved in energy transfer. htes 3 _22g2 KIAA0829 is shorter, nearly Involved in TATA box binding complex Nucleic Acid identical to rat TIP120 Management htes 3 2ml8 Nearly identical to mouse Dhml Multifunctional nuclease/exoribonuclease Nucleic acid management htes3_7p9 Similarity to nuclear domain 10 Transcription control Nucleic Acid protein NDP52 Management htes3 8ml0 Strong similarity to The poly (A) -binding protein (PABP) binds to the messenger (mRNA) 3 '-poly (A) tail Nucleic Acid polyadenylate-binding proteins. found on most eukaryotic mRNAs and together with the poly (A) tail has been Management implicated in governing the stability and the translation of mRNA. hutel 1811 Strong similarity to S. cerevisiae Mitochondrianl Ribosomal S40 protein Nucleic Acid YHR148w Management Group testis associated htes3 2m20 Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motife. Testes associated htes3 2n9 Very weak similarity to Homo No informative BLAST results; No predictive prosite, pfam or SCOP motife. Testes sapiens PAC clone DJ0771P04 from associated 7qll.21-qll.23. htes3 30f4 Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motife. Testes associated htes 3 _35g6 Strong similarity to R27216_l No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes associated htes3 35n24 Unknown Contains an IG_MHC pattern Testes No informative BLAST results; No predictive prosite, pfam or SCOP motive associated htes3_35pl7 Similarity to S. cerevisiae VAC8 No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes and beta-Catenin, but contains no associated a adillo motifes htes3 4b4 Rattus norvegicus late gestation No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes lung protein 1 associated htes3 4fl7 Similarity to KIAA03 33 No informative BLAST results,- No predictive prosite, pfam or SCOP motive Testes Methyl-CpG binding protein,- does associated not contain such a motife. htes3 4ol9 Similarity to mucin No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes associated htes3_50j4 Unknown, prolin rich protein No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes associated o htes 3 50n2 3 Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes cn associated htes3 50n6 Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes associated htes 3 6b21 Similarity to KIAA0256 No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes associated htes3 6dl6 WUGSC:H_DJ1185I07.2, differences No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes to genmodel associated htes3 72kll Similarity to S.pombe hypothetical No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes repeat-containing protein associated htes3 7dl7 Similarity to KIAA0454 No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes associated htes3_7j8 WUGSC :H_DJ1159O04.1 similarity to No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes YBL104p associated htes3_8gll Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes associated htes3_8g5 KIAA087, alternative spliced No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes associated htes3_8p7 Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes associated htes3 9e22 Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes associated htes3 9i20 Unknown No informative BLAST results,- No predictive prosite, pfam or SCOP motive Testes associated htes3 9k22 Similarity to C-terminus of No informative BLAST results; No predictive prosite, pfam or SCOP motive Testes katanin p80 associated Group transmembrane proteins hfbr2 16il2 Similarity to Fugu rubripes PUT2 1 transmembrane domain Transmembran No informative BLAST results; No predictive prosite, pfam or SCOP motife. e protein hfbr2 16112 Similarity to gallus putative 1 transmembrane domain Transmembran trans embranee protein E3-16 No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein hfbr2 22hl3 Similarity to Drosophila 1 transmembrane domain Transmembran melanogaster EG:39E1.3. No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein hfbr2 2bl7 Similarity to Drosophila 3 transmembrane domains Transmembran hypothetical 3OK protein No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein hfbr2 2dl7 Unknown 1 transmembrane domain Transmembran No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein hfbr2 64k24 Similarity to several proteins 5 transmembrane regions. Transmembran No informative BLAST results; No predictive prosite, pfam or SCOP motife. e protein hfbr2 82C20 Similarity to C. elegans D1007.5 7 transmembrane domains Transmembran No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein hfbr2 82el7 Similarity to C. elegans "R01B10.5" 6 transmembrane domains Transmembran No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein hfbr2_82gl4 Unknown proline rich protein 1 transmembrane domain Transmembran No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein hfkd2 24al5 Similarity to C. elegans R07G3.8 1 transmembrane domain Transmembran No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein hfkd2 3il3 Similarity to A. thaliana YUP8H12.2 3 transmembrane domains Transmembran No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein hfkd2 4mll Weak similarity to YMR034C 4 transmembrane domains Transmembran No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein hmcfl lall Similarity to YDR255C and 1 transmembrane domain Transmembran SPBC29A3.03C No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein hmcfl lel5 Similarity to D-XYLOSE TRANSPORTER Transporter; Transmembran 9 transmembrane domains e protein No informative BLAST results; No predictive prosite, pfam or SCOP motive htes3 15c6 Unknown 1 transmembrane domain Transmembran No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein htes3 2ol3 Partial similarity to the IL-17 1 transmembrane domain Transmembran receptor . No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein htes3 27k4 Strong similarity to C. elegans Contains a leucine zipper Transmembran K07H8.2/ZK185.2 10 transmembrane domains e protein No informative BLAST results; No predictive prosite, pfam or SCOP motive; htes3 2hl Similarity to C. elegans C13F10.5 1 transmembrane domain Transmembran No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein htes 3 35k24 Unknown 5 transmembrane domains Transmembran No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein hutel 19fl9 Similarity to mouse P24 protein 2 transmembrane domains Transmembran No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein hutel 24cl9 Unknown 1 transmembrane domain Transmembran No informative BLAST results; No predictive prosite, pfam or SCOP motive e protein Group Brain derived hfbr2 64cl6 Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motive Brain derived hfbr2 64C4 Similarity to A. thaliana T08I13.5 No informative BLAST results; No predictive prosite, pfam or SCOP motive Brain derived hfbr2 64h6 Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motive Brain derived hfbr2 64i20 Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motive Brain derived hfbr2 64ol6 Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motive Brain derived hfbr2 6al7 Weak similarity to finger protein No informative BLAST results; No predictive prosite, pfam or SCOP motive Brain zfOCl derived hfbr2 6i20 Similarity to ribosomal protein No informative BLAST results; No predictive prosite, pfam or SCOP motive Brain L15 precursor, mitochondrial derived hfbr2 71O20 Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motive Brain derived hfbr2 72dl3 Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motive Brain derived hfbr2 72ml6 Similarity to C. elegans H14A12.3 No informative BLAST results; No predictive prosite, pfam or SCOP motive Brain derived hfbr2 72nl2 Strong similarity to rat No informative BLAST results; No predictive prosite, pfam or SCOP motive Brain Ganglioside expression factor derived GEF-2) but even higher identity with C. elegans putative protein Identities = 91/116 (78%) hfbr2 78dl3 Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motive Brain derived hfbr2 78n23 Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motive Brain derived hfbr2 7a24 DKFZphfbr2_7a24.1 similarity to C- Only c-terminus homolog; contains no kinase domain; Brain terminus of TGF-beta-activated No informative BLAST results; No predictive prosite, pfam or SCOP motive derived kinase hfbr2 7e22 Similarity to cytochrome b561 No heme domain but a c may helix loop helix signature Brain No informative BLAST results; No predictive prosite, pfam or SCOP motive derived hfbr2_7j4 Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motive Brain derived hfbr2 82ml6 Very weak similarity to A. thaliana No informative BLAST results; No predictive prosite, pfam or" SCOP motive Brain F28A23.140 derived Group Intracellular Transport and Trafficking o KΩ Group signal transduction hutel 22el2 Strong similarity to S. cerevisiae The Drosophila cni and mammalian proteins cornicon are part of a signal Signal YGL054C and cornichon transduction pathway involving hte EGF-receptor transduction Group Metabolism Group transcription factors hfkd2 46kl9 Strong similarity to pterin-4- Dcoh is a bifunctional protein, complexed with biopterin. It serves as Transcriptio alpha-carbinolamine dehydratase dimerization cofactor of hepatocyte nuclear factor-1 and catalyzes the dehydration n factor of the biopterin cofactor of phenylalanine hydroxylase hfkd2 47a4 Similarity to zinc fingers New putative transcription factor with one C2H2 zinc fingers. Transcriptio n factor htes3 2el2 Similarity to finger proteins Transcription factor with three C2H2 zinc fingers. Additionally, a cytochrome C Transcripton family heme-binding site signature is present in the protein factros htes 3 _21j l5 3 strong similarity to "NY-CO-33" Transcription factor Transcriptio n factors htes3 17nl2 Nearly identical to mouse SOX-LZ SOX-LZ, related to SRY and HMG-box-Proteins Transcriptio n factors hutel 18il9 Similarity to transcription factor The SREBP-2 protein is cleaved to release soluble NH2-terminal that enter the Transcriptio SF3 nucleus and activate genes encoding the low density lipoprotein receptor and n factor enzymes of cholesterol synthesis; a lim domain; shows similarity to the common sunflower transcripti hutel li2 Similarity to Dictostelium myosin Zn- finger protein Transcriptio heavy chain kinase n factor O ro Group uterus associated ftthiJΛ hutel 17k7 Similarity to HPBRII-4 MRNA No informative BLAST results; No predictive prosite, pfam or SCOP motive Uterus associated hutel 18cl2 Similarity to candidate tumor No informative BLAST results; No predictive prosite, pfam or SCOP motive Uterus suppressor p 33 INGl associated hutel 18i4 Weak similarity to C. elegans No informative BLAST results; No predictive prosite, pfam or SCOP motive Uterus D2085.2 associated hutel_19gl9 Partial similarity to bovine No informative BLAST results; No predictive prosite, pfam or SCOP motive Uterus elastin fragment associated hutel_19jll Strong similarity to KIAA02 3 l, No informative BLAST results; No predictive prosite, pfam or SCOP motive Uterus similarity to ras binding protein associated Sur8 hutel 22n2 Similar to F46F6.1 No informative BLAST results; No predictive prosite, pfam or SCOP motive Uterus associated hutel 21dl5 Unknown No informative BLAST results; No predictive prosite, pfam or SCOP motive Uterus associated hutel 22o2 Similarity to S. ombe SPBC3E7.03c No informative BLAST results; No predictive prosite, pfam or SCOP motive Uterus associated hutel_23gll Similarity to SPAC31G5.12C and No informative BLAST results; No predictive prosite, pfam or SCOP motive Uterus Maflp associated O co Prosite Key NAME' N-glycosylation site. CONSENSUS. N-{P}-[ST]-{P} NAME Glycosaminoglycan attachment site. CONSENSUS: S-G-x-G. NAME Tyrosine sulfation site NAME: cAMP- and cGMP-dependent protein kinase phosphorylation site CONSENSUS: [RK](2)-x-[ST]. NAME: Protein kinase C phosphorylation site CONSENSUS: [ST]-x-[RK]. NAME: Casein kinase II phosphorylation site CONSENSUS- [ST]-x(2)-[DE]. NAME: Tyrosine kinase phosphorylation site CONSENSUS. [RK]-x(2,3)-[DE]-x(2,3)-Y NAME: N-myπstoylation site. CONSENSUS. G-{EDRKHPFYW}-x(2)-[STAGCN]-{P} NAME: Amidation site CONSENSUS: x-G-[RK]-[RK]. NAME: Aspartic acid and asparagine hydroxylation site CONSENSUS C-x-[DN]-x(4)-[FY]-x-C-x-C NAME: Vitamin K-dependent carboxylation domain CONSENSUS' x( 12)-E-x(3)-E-x-C-x(6)-[DEN]-x-[LIVMFY]-x(9)-[FYW] NAME. Phosphopantetheine attachment site. CONSENSUS [DEQGSTALMKRH]-[LrVMFYSTAC]-[GNQ]-[ IVMFYAG]-[DNEKHS]-S-[LIVMST]- CONSENSUS. {PCFY}-[STAGCPQLIVMF]-[LIVMATN]-[DENQGTAKRHLM]-[ IVMWSTA]-[LIVGSTACR]- CONSENSUS x(2)-[LIVMFA). NAME- Acyl earner protein phosphopantetheine domain profile NAME: Prokaryotic membrane lipoprotein lipid attachment site CONSENSUS {DERK}(6)-[LIVMFWSTAG](2)-[LIVMFYSTAGCQ]-[AGS]-C . NAME: Prokaryotic N-terminal methylation site CONSENSUS: [KRHEQSTAG]-G-[FY IVM]-[ST1-[LT1-[LIVP]-E-[LIVMF STAG](14). NAME: Prenyl group binding site (CAAX box) CONSENSUS: C-{DENQ}-[LIVM]-x> . NAME: Protein splicing signature. CONSENSUS. [DNEG]-x-[LIVFA]-[LrVMY]-[LVAST]-H-N-[STC] NAME: Endoplasmic reticulum targeting sequence CONSENSUS: [KRHQSA]-[DENQ]-E-L > . NAME Microbodies C-terminal targeting signal. CONSENSUS: [STAGCN]-[RKH]-[LIVMAFY] > . NAME: Gram-positive cocci surface proteins 'anchoring' hexapeptide CONSENSUS: L-P-x-T-G-[STGAVDE]. NAME: Bipartite nuclear targeting sequence. NAME: Cell attachment sequence. CONSENSUS: R-G-D. NAME ATP/GTP-binding site motif A (P-loop). CONSENSUS. [AG]-x(4)-G-K-[ST]. NAME Cyclic nucleotide-binding domain signature 1. CONSENSUS [LIVM]-[VIC]-x(2)-G-[DENQTA]-x-[GAC]-x(2)-[LIVMFY]( )-x(2)-G. NAME Cyclic nucleotide-binding domain signature 2 CONSENSUS [LIVMF]-G-E-x-[GAS]-[LIVM]-x(5, 1 l)-R-[STAQ]-A-x-[LIVMA]-x-[STACV] NAME cAMP/cGMP binding motif NAME EF-hand calcium-binding domain CONSENSUS D-x-[DNS]-{ILVFYW}-[DENSTG]-[DNQGHRK]-{GP}-[LIVMC]-[DENQSTAGC]-x(2)- CONSENSUS [DEHLIVMFYW] NAME Actinin-type actin-binding domain signature 1 CONSENSUS [EQ]-x(2)-[ATV]-[FY]-x(2)-W-x-N NAME Actimn-type actin-binding domain signature 2 CONSENSUS [LIVM]-x-[SGN]-[LIVM]-[DAGHE]-tSAG]-x-[DNEAG]-[LIVM]-x-[DEAG]-x(4)- CONSENSUS [LIVM]-x-[LM]-[SAG]-[LIVM]-[LIVMT]-W-x-[LIVM](2) NAME Anaphylatoxin domain signature CONSENSUS [CSH]-C-x(2)-[GAP]-x(7,8)-[GASTDEQR]-C-[GASTDEQL]-x(3,9)-[GASTDEQN]-x(2>- CONSENSUS [CE]-x(6,7)-C-C NAME Anaphylatoxin domain profile NAME Apple domam CONSENSUS C-x(3)-[ IVMFY]-x(5)-[LIVMFY]-x(3)-[DENQ]-[LIVMFY]-x(10)-C-x(3)-C-T- CONSENSUS x(4)-C-x-[LrVMFY>F-x-[FY]-x(13,14)-C-x-[LIVMFYHRK]-x-[ST]-x(14,15)- CONSENSUS S-G-X-[STJ-[ ΓVMFY] X(2)-C NAME Band 4 1 family domain signature 1 CONSENSUS W-[LIV]-x(3)-[KRQ]-x-[LIVM]-x(2)-[QH]-x(0,2)-[ IVMF]-x(6,8)-[LIVMF]- CONSENSUS x(3,5)-F-[FY]-x(2)-[DENS] NAME Band 4 1 family domain signature 2 CONSENSUS [HYW]-x(9)-[DENQSTV]-[SA]-x(3)-[FY]-[LIVM]-x(2)-[ACV]-x(2)-[LM]-x(2)- CONSENSUS [FY]-G-x-[DENQSTHLIVMFYS] NAME Band 4 1 family domain profile NAME Clq domam signature CONSENSUS F-x(5) [ND]-x(4)-[FYWL]-x(6)-F-x(5)-G-x-Y-x-F-x-[FY] NAME C-terminal cystine knot signature CONSENSUS C-C-x(13)-C-x(2)-[GN]-x(12)-C-x-C-x(2,4)-C NAME C-terminal cystine knot profile NAME CUB domain profile NAME Death domain profile NAME EGF- ke domam signature 1 CONSENSUS C-x-C-x(5)-G-x(2)-C NAME EGF-hke domain signature 2 CONSENSUS C-x-C-x(2)-[GP]-[FYW]-x(4,8)-C NAME Calcium-binding EGF-hke domam pattern signature CONSENSUS [DEQN]-x-[DEQN](2)-C-x(3,14)-C-x(3,7)-C-x-[DN]-x(4)-[FY]-x-C NAME Laminin-type EGF-hke (LE) domain signature CONSENSUS C-x(l ,2)-C-x( 5 )-G-x(2)-C-x(2)-C-x(3,4)-[FYW]-x(3, 15)-C NAME Coagulanon factors 5/8 type C domain (FA58C) signature 1 CONSENSUS [GAS]-W-x(7,l 5 )-[Fj W]-[LrV]-x-[LIVFA]-[GSTDEN]-x(6)-[LIVF]-x(2)-[IV]-x- CONSENSUS [LIVT]-[QKM]-G NAME Coagulation factors 5/8 type C domain (FA58C) signature 2 CONSENSUS P-x(8, 10)-[LM]-R-x-[GE]-[LIVP]-x-G-C NAME Forkhead-associated (FHA) domain profile NAME Fibrinogen beta and gamma chains C-terminal domain signature CONSENSUS W-W-[LIVMFY ]-x(2)-C-x(2)-[GSA]-x(2)-N-G NAME Type I fϊbronectin domam CONSENSUS C-x(6,8)-[LFY]-x(5)-[FYW]-x-[RK]-x(8,10)-C-x-C-x(6,9)-C NAME Type II fibronectin collagen-binding domain CONSENSUS C-x(2)-P-F-x-[FYWI]-x(7)-C-x(8,10)-W-C-x(4)-[DNSR]-[FYW -x(3,5)-[FYW]-x- CONSENSUS [FYWI]-C NAME Hemopexin domain signature CONSENSUS [LIFAT]-x(3)-W-x(2,3)-[PE]-x(2)-[LIVMFY]-[DENQS]-[STA]-[AV]-[LIVMFY] NAME Kπngle domain signature CONSENSUS [FY]-C-R-N-P-[DNR] NAME Kπngle domain profile NAME LDL-receptor class A (LDLRA) domain signature CONSENSUS C-[VILMAJ-x(5)-C-[DNH]-x(3)-[DENQHT]-C-x(3,4)-[STADE]-[DEH]-[DEJ-x( 1 ,5)- CONSENSUS C NAME LDL-receptor class A (LDLRA) domain profile NAME C-type lectin domain signature CONSENSUS C-tLIVMFYATG]-x(5,12)-[WL]-x-[DNSR]-x(2)-C-x(5,6)-[FYWLIVSTA]-[LIVMSTA]- CONSENSUS C NAME C-type lectin domain profile NAME Link domain signature CONSENSUS C-x(15)-A-x(3,4)-G-x(3)-C-x(2)-G-x(8,9)-P-x(7)-C NAME Osteonectin domain signature 1 CONSENSUS C-x-[DN]-x(2)-C-x(2)-G-[KRH]-x-C-x(6,7)-P-x C-x-C-x(3,5)-C-P NAME Osteonectin domam signature 2 CONSENSUS F-P-x-R-[IM]-x-D-W-L-x-[NQ] NAME Somatomedin B domam signature CONSENSUS C-x-C-x(3)-C-x( 5 )-C-C-x-[DN]-[FY]-x(3)-C NAME Thyroglobulin type-1 repeat signature CONSENSUS [FYWHP]-x-P-x-C-x(3,4)-G-x-[FY ]-x(3)-Q-C-x(4,10)-C-[FYW]-C-V-x(3,4)- CONSENSUS [SG] NAME P-type 'Trefoil' domain signature CONSENSUS R-x(2)-C-x-[FYPST]-x(3,4)-[ST]-x(3)-C-x(4)-C-C-[FYWH] NAME Cellulose-binding domain, bactenal type CONSENSUS W-N-[STAGR]-[STDN]-[LIVM]-x(2)-[GST]-x-[GST]-x(2)-[LrVMFT]-[GA] NAME Cellulose-binding domain, fungal type CONSENSUS C-G-G-x(4,7)-G-x(3)-C-x(5)-C-x(3,5)-[NHG]-x-[FYWM]-x(2)-Q-C NAME Cnitin recognition or binding domain signature CONSENSUS C-x(4,5)-C-C-S-x(2)-G-x-C-G-x(4)-[FYW]-C NAME Barwin domain signature 1 CONSENSUS C-G-[KR]-C-L-x-V-x-N NAME Barwin domain signature 2 CONSENSUS V-[DN]-Y-[EQ]-F-V-[DN]-C NAME BIR repeat CONSENSUS [HKEPILVY]-x(2)-R-x(3,7)-[FYW]-x(l 1 , 14)-[STAN]-G-[LMF]-X-[FYHDA]-X(4)- CONSENSUS [DESL]-X(2,3)-C-X(2)-C-X(6)-[WA]-X(9)-H-X(4)-[PRSD]-X-C-X(2)-[LIVMA] NAME WAP-type 'four-disulfide core' domam signature CONSENSUS C-x-{C}-[DN]-x(2)-C-x(5)-C-C NAME Phorbol esters / diacylglycerol binding domain CONSENSUS H-x-[LIVMFYW]-x(8, 1 l)-C-x(2)-C-x(3)-[LIVMFC]-x(5, 10)-C-x(2)-C-x(4)-[HD]- CONSENSUS x(2)-C-x(5,9)-C NAME C2 domain signature CONSENSUS [ACG]-x(2)-L-x(2,3)-D-x(l,2)-[NGSTLIF]-[GTMR]-x-[STAP]-D-[PA]-[FY] NAME C2 -domain profile NAME CAP-Gly domain signature CONSENSUS G-x(8,10)-[FYW]-x-G-[LIVM]-x-[LIVMFY]-x(4)-G-K-[NH]-x-G-[STAR]-x(2)-G- CONSENSUS x(2)-[LY]-F NAME Ly-6 / u-PAR domain signature CONSENSUS [EQR]-C-[LIVMFYAH]-x-C-x(5,8)-C-x(3,8)-[EDNQSTV]-C-{C}-x(5)-C- CONSENSUS x(12,24)-C NAME MAM domain signature CONSENSUS G-x-[LIVMFY](2)-x(3)-[STA]-x(10, 1 l)-[LV]-x(4)-[LIVMF]-x(6,7)-C-[LIVM]-x- CONSENSUS F-x-[LIVMFY]-x(3)-[GSC] NAME MAM domain profile NAME PH domain profile NAME Phosphotyrosine interaction domain (PID) profile NAME Src homology 2 (SH2) domain profile NAME Src homology 3 (SH3) domain profile NAME VWFC domain signature CONSENSUS C-x(2,3)-C-x-C-x(6,14)-C-x(3,4)-C-x(2,10)-C-x(9,16)-C-C-x(2,4)-C NAME W /rsp5/WWP domain signature CONSENSUS W-x(9, 1 l)-[VFY]-[FYW]-x(6,7)-[GSTNE]-[GSTQCR]-[FYW]-x(2) P NAME W /rsp5/WWP domain profile NAME ZP domain signature CONSENSUS [LIVMFYW]-x(7)-[STAPDNL]-x(3)-[LIVMFVVV]-x-[LIVMFYW]-x-[LrVMFYW]-x(2)-C- CONSENSUS [LIVMFYW]-x-[ST]-[PSL]-x(2,4)-[DENS]-x-[STADNQLF]-x(6)-[LIVM](2)-x(3,4)- CONSENSUS C NAME S-layer homology domain signature CONSENSUS [LVFYT]-x-[DA]-x(2,5)-[DNGSATPHY]-[WYFPDA]-x(4)-[LIV]-x(2)-[GTALV]- CONSENSUS x(4,6)-[LIVFYC]-x(2)-G-x-[PGSTA]-x(2,3)-[MFYA]-x-[PGAV]-x(3,10)-[LIVMA]- CONSENSUS [STKR]-[RY]-x-[EQ]-x-[STALIVM] NAME 'Homeobox' domain signature CONSENSUS [LIVMFYG]-[ASLVR]-x(2)-[LIVMSTACN]-x-[LIVM]-x(4)-[LIV]-[RKNQESTAIY]- CONSENSUS [LIVFSTNKH]-W-[FYVC]-x-[NDQTAH]-x(5)-[R NAIMW] NAME 'Homeobox' domain profile NAME 'Homeobox' antennapedia-type protein signature CONSENSUS [LIVMFE]-[FY]-P-W-M-[KRQTA] NAME 'Homeobox' engrailed-type protein signature CONSENSUS L-M-A-Q-G-L-Y-N NAME 'Paired box' domain signature CONSENSUS R-P-C-x( 11 )-C-V-S NAME 'POU' domain signature 1 CONSENSUS [RKQ]-R-[LIM]-x-[LF]-G-[LIVMFY]-x-Q-x-[DNQ]-V-G NAME 'POU' domain signature 2 CONSENSUS S-Q-[ST]-[TA]-I-[SC]-R-F-E-x-[LSQ]-x-[LI]-[ST] NAME Zinc finger, C2H2 type, domam CONSENSUS C-x(2,4)-C-x(3)-[LIVMFYWC]-x(8)-H-x(3,5)-H NAME Zinc finger, C3HC4 type (RING finger), signature CONSENSUS C-x-H-x-[LIVMFY]-C-x(2)-C-[LIVMYA] NAME Nuclear hormones receptors DNA-bindmg region signature CONSENSUS C-x(2)-C-x-[DE]-x(5)-[HN]-[FY]-x(4)-C-x(2)-C-x(2)-F-F-x-R NAME GATA-type zinc finger domain CONSENSUS C-x-[DN]-C-x(4,5)-[ST]-x(2)-W-[HR]-[RK]-x(3)-[GN]-x(3,4)-C-N-[AS]-C NAME Poly(ADP-nbose) polymerase zinc finger domain signature CONSENSUS C-[KR]-x-C-x(3)-I-x-K-x(3)-[RG]-x(16,18)-W-[FYH]-H-x(2)-C NAME Poly(ADP-πbose) polymerase zmc finger domain profile NAME Fungal Zn(2)-Cys(6) binuclear cluster domain signature CONSENSUS [GASTPV]-C-x(2)-C-[RKHSTACW]-x(2)-[RKHQ]-x(2)-C-x(5,12)-C-x(2)-C-x(6,8)- CONSENSUS C NAME Fungal Zn(2)-Cys(6) bmuclear cluster domain profile NAME Prokaryotic dksA/traR C4-type zinc finger CONSENSUS C-[DES]-x-C-x(3)-I-x(3)-R-x(4)-P-x(4)-C-x(2)-C NAME Copper-fist domain signature CONSENSUS M-[LIVMF](3)-x(3)-K-[MY]-A-C-x(2)-C-I-[KR]-x-H-[KR]-x(3)-C-x-H-x(8)- CONSENSUS [KR]-x-[KR]-G R-P NAME Copper fist DNA binding domain profile NAME Leucine zipper pattern CONSENSUS L-x(6)-L-x(6)-L-x(6)-L NAME bZIP transcription factors basic domain signature CONSENSUS [KR]-x( 1 ,3)-[RKSAQ]-N-x(2)-[SAQ](2)-x-[RKTAENQ}-x-R-x-[RK] NAME Myb DNA-binding domain repeat signature 1 CONSENSUS W-[ST]-x(2)-E-[DE]-x(2)-[LIV] NAME Myb DNA-binding domam repeat signature 2 CONSENSUS W-x(2)-[LI]-[SAG]-x(4,5)-R-x(8)-[YWl-x(3)-[LrVM] NAME Myc-type, 'helix-loop-helix' dimerization domain signature CONSENSUS [DENSTAP]-K [LIVMWAGSN]-{FYWCPHKR}-[LIVT]-[LIV]-x(2)-[STAV]-[LIVMSTAC]-x- CONSENSUS [VMFYH]-[LIVMTA]-{P}-{P}-[LIVMSR] NAME p53 tumor antigen signature CONSENSUS M-C-N-S-S-C-M-G-G-M-N-R-R NAME CBF-A/NF-YB subunit signature CONSENSUS C-V-S-E-x-I-S-F-[LIVM]-T-[SG]-E-A-[SC]-[DE]-[KRQ]-C NAME CBF-B/NF-YA subunit signature CONSENSUS Y-V-N-A-K-Q-Y-x-R-I-L-K-R-R-x-A-R-A-K-L-E NAME 'Cold-shock' DNA-bindmg domain signature CONSENSUS [FY]-G-F-I-x(6,7)-[DER]-[LIVM]-F-x-H-x-[STKR]-x-[LIVMFY] NAME CTF/NF-I signature CONSENSUS R-K-R-K-Y-F-K-K-H-E-K-R NAME Ets-domain signature 1 CONSENSUS L-[FYW]-[QEDH]-F-[LI]-[LVQK]-x-[Lη-L NAME Ets-domain signature 2 CONSENSUS [RKH]-x(2)-M-x-Y-[DENQ]-x-[LIVM]-[STAG]-R-[STAG]-[LI]-R-x-Y NAME Ets-domain profile NAME Fork head domam signature 1 CONSENSUS [KR]-P-[PTQ]-[FYLVQH]-S-[FY]-x(2)-[LIVM]-x(3,4)-[AC]-[LIM] NAME Fork head domain signature 2 CONSENSUS W-[QKR]-[NS]-S-ΓLIV]-R-H NAME Fork head domain profile NAME HSF-type DNA-binding domain signature CONSENSUS L-x(3)-[FY]-K-H-x-N-x-[STAN]-S-F-[LrVM]-R-Q-L-[NH]-x-Y-x-[FYW -[RKH]-K- CONSENSUS [LIVM] NAME Tryptophan pentad repeat (IRF family) signature CONSENSUS W-x-[DNH]-x(5)-[LIVF]-x-[IV]-P-W-x-H-x(9,10)-[DE]-x(2)-[LIVF]-F-[KRQ]-x- CONSENSUS [WR]-A NAME LIM domain signature CONSENSUS C-x(2)-C-x(15,21)-[FYWH]-H-x(2)-[CH]-x(2)-C-x(2)-C-x(3)-[LIVMF] NAME LIM domain profile NAME NF-kappa-B/Rel/dorsal domain signature CONSENSUS F-R-Y-x-C-E-G NAME MADS-box domain signature CONSENSUS R-x-[RK]-x(5)-I-x-[DN]-x(3)-[KR]-x(2)-T-[FY]-x-[RK](3)-x(2)-[LIVM]-x- CONSENSUS K(2)-A-x-E-[LIVM]-[ST]-x-L-x(4)-[LIVM]-x-[LIVM](3)-x(6)-[LIVMF]-x(2)- CONSENSUS [FY] NAME MADS-box domain profile NAME T-box domain signature 1 CONSENSUS L-W-x(2)-[FC]-x(3,4)-[NT]-E-M-[LIV](2)-T-x(2)-G-[RG]-[KRQ] NAME T-box domain signature 2 CONSENSUS [LIVMYW]-H-[PADH]-[DEN]-[GS]-x(3)-G-x(2)-W-M-x(3)-[IVA]-x-F NAME TEA domain signature CONSENSUS G-R-N-E-L-I-x(2)-Y-I-x(3)-[TC]-x(3)-R-T-[RK](2)-Q-[LIVM]-S-S-H-[LIVM]- CONSENSUS Q-V NAME Transcnption factor TFIIB repeat signature CONSENSUS G-[KR]-x(3)-[STAGN]-x-[LIVMYA]-[GSTA](2)-[CSAV]-[LIVM]-[LIVMFY]-[LIVMA]- CONSENSUS [GSAHSTAC] NAME Transcription factor TFIID repeat signature CONSENSUS Y-x-P-x(2)-[IF]-x(2)-[LIVM](2)-x-[KRH]-x(3)-P-[RKQ]-x(3)-L-[LIVM]-F-x- CONSENSUS [STN]-G-[KR]-[LIVM]-x(3)-G-[TAGL]-[KR]-x(7)-[AGC]-x(7)-[LIVM] NAME TFIIS zinc ribbon domain signature CONSENSUS C-x(2)-C-x(9)-[LIVMQSAR]-[QH]-[STQL]-[RA]-[SACR]-x-[DE]-[DET]-[PGSEA]- CONSENSUS x(6)-C-x(2,5)-C-x(3)-[FW] NAME TSC-22 / dip / bun family signature CONSENSUS M-D-L-V-K-x-H-L-x(2)-A-V-R-E-E-V-E NAME Prokaryotic transcription elongation factors signature 1 CONSENSUS [ST]-x(2)-[GS]-x(3)-[LI]-x(2)-E-L-x(2)-L-x(3,4)-R-x(2)-[IV]-x(3)-[LIV]- CONSENSUS x(6)-G-D-x(2)-E-N-[GSA]-x-Y NAME Prokaryotic transcnption elongation factors signature 2 CONSENSUS S-x(2)-S-P-[LIVM]-[AG]-x-[SAG]-[LIVM]-[LIVMY]-x(4)-[DG]-[DE] NAME DEAD-box subfamily ATP-dependent helicases signature CONSENSUS tLrVMF](2)-D-E-A-D-[RKEN]-x-[LIVMFYGSTN] NAME DEAH-box subfamily ATP-dependent helicases signature CONSENSUS [GSAH]-x-[LIVMF](3)-D-E-[ALIV]-H-[NECR] NAME Eukaryotic putative RNA-binding region RNP-1 signature CONSENSUS [RK]-G-{EDRKHPCG}-[AGSCI]-[FY]-[LIVA]-x-[FYLM] NAME Fibπllaπn signature CONSENSUS [GST]-[LIVMAP]-V-Y-A-[TV]-E-[FY]-[SA]-x-R-x(2)-R-[DE] NAME MCM family signature CONSENSUS G-[IVT]-[LVAC](2)-[IVT]-D-[DE]-[FL]-[DNST] NAME MCM family domain NAME XPA protein signature 1 CONSENSUS C-x-[DE]-C-x(3)-[LIVMF]-x( 1 ,2)-D-x(2)-L-x(3)-F-x(4)-C-x(2)-C NAME XPA protein signature 2 CONSENSUS [LIVM](2)-T-[KR]-T-E-x-K-x-[DE]-Y-[LIVMF](2)-x-D-x-[DE] NAME XPG protein signature 1 CONSENSUS [VI]-[KRE]-P-x-[FYIL]-V-F-D-G-x(2)-[PIL]-x-[LVC]-K NAME. XPG protein signature 2 CONSENSUS: [GS]-[LIVM]-[PER]-[FYS]-[LIVM]-x-A-P-x-E-A-[DE]-[PAS]-[QS]-[CLM] NAME Bacterial regulatory proteins, araC family signature. CONSENSUS: [KRQ]-[LIVMA]-x(2)-[GSTALIV]-{FYWPGDN}-x(2)-[LIVMSA]-x(4,9)-[LIVMF]- CONSENSUS: x(2)-[LIVMSTA]-[GSTACIL]-x(3)-[GANQRF]-[LIVMFY]-x(4,5)-[LFY]-x(3)- CONSENSUS- [FYIVA]-{FYWHCM}-x(3)-[GSADENQKR]-x-[NSTAPKL]-[PARL]. NAME: Bacteπal regulatory proteins, araC family DNA-bindmg domain profile. NAME- Bacterial regulatory proteins, arsR family signature CONSENSUS: C-x(2)-D-[LIVM]-x(6)-[ST]-x(4)-S-[HYR]-[HQ] NAME: Bacterial regulatory proteins, asnC family signature. CONSENSUS- [GSTAP]-x(2)-[DNEA]-[LIVM]-[GSA]-x(2)-[LIVMFY]-[GN]-[LIVMST]-[ST]-x(6)-R- CONSENSUS: [LVT]-x(2)-[LIVM]-x(3)-G NAME: Bacterial regulatory proteins, crp family signature. CONSENSUS: [LIVM]-[STAG]-[RHNW]-x(2)-[LIM]-[GA]-x-[LIVMFYA]-[LIVSC]-[GA]-x-[STACN]- CONSENSUS: x(2)-[MST]-x-[GSTN]-R-x-[LIVMF]-x(2)-[LIVMF] . NAME: Bacterial regulatory proteins, deoR family signature CONSENSUS- R-x(3)-[LIVM]-x(3)-[LIVM]-x(16,17)-[STA]-x(2)-T-[LIVMA]-[RH]-[KRNA]-D- CONSENSUS: [LIVMF]. NAME: Bacterial regulatory proteins, gntR family signature CONSENSUS- [LIVAPKR]-[PILV]-x-[EQTIVMR]-x(2)-[LIVM]-x(3)-[LIVMFYK]-x-[LIVFT]- CONSENSUS. [DNGSTK]-[RGTLV]-x-[STAIVP]-rLIVA]-x(2)-[STAGV]-[LIVMFYH]-x(2)-[LMA]. NAME Bacterial regulatory proteins, ICIR family signature CONSENSUS: [GA]-x(3)-[DS]-x(2)-E-x(6)-[CSA]-[LIVM]-[GSA]-x(2)-[LIVM]-[FYH]-[DN]. NAME: Bacterial regulatory proteins, lad family signature CONSENSUS [LIVM]-x-[DE]-[LIVM]-A-x(2)-[STAGV]-x-V-[GSTP]-x(2)-[STAG]-[LIVMA}-x(2)- CONSENSUS [LIVMFYANHLIVMC] NAME Bactenal regulatory proteins, luxR family signature. CONSENSUS: [GDC]-x(2)-[NSTAVY]-x(2)-[IV]-[GSTA]-x(2)-[LIVMFYWCT]-x-[LIVMFYWCR]-x(3)- CONSENSUS: [NST]-[LIVM]-x(5)-[NRHSA]-[LIVMSTA]-x(2)-[KR] NAME Bacteπal regulatory proteins, lysR family signature. CONSENSUS [NQKRHSTAG]-[LIVMFYTA]-x(2)-[STAGLV]-[STAG]-x(4)-[LIVMYCTQR]-[PSTANLVER]- CONSENSUS x-[PSTAGQV]-[PSTAGNVMF]-[LIVMFA]-[STAGH]-x(2)-[LIVMF]-x(2)-[LIVMFW]- CONSENSUS: [RKEAV]-x(2)-[LIVMFYNTAE]-x(3)-[LIMVT] . NAME. Bactenal regulatory proteins, marR family signature. CONSENSUS: [STNA]-[LIA]-x-[RNGS]-x(4)-[LM]-[EIV]-x(2)-[GES]-[LFYW]-[LIVC]-x(7)- CONSENSUS: [DN]-[RKQG]-[RK]-x(6)-T-x(2)-[GA] NAME: Bactenal regulatory proteins, merR family signature. CONSENSUS. [GSA]-x-[LIVMFA]-[ASM]-x(2)-[STACLIV]-[GSDENQR]-[LIVC]-[STANHK]-x(3)- CONSENSUS [LIVM]-[RHF]-x-[YW]-[DEQ]-x(2,3)-[GHDNQ]-[LIVMF](2). NAME: Bactenal regulatory proteins, tetR family signature. CONSENSUS G-[LIVMFYS]-x(2,3)-[TS]-[LIVMT]-x(2)-[LIVM]-x(5)-[LIVQS]-[STAGENQH]-x- CONSENSUS- [GPAR]-x-[LIVMF]-[FYST]-x-[HFY]-[FV]-x-[DNST]-K-x(2)-[LIVM]. NAME' Transcnptional antiterminators bglG family signature. CONSENSUS' [ST]-x-H-x(2)-[FA](2)-[LIVM]-[EQK]-R-x(2)-[QNK]. NAME- Sιgma-54 factors family signature 1. CONSENSUS: P-[LIVM]-x-tLIVM]-x(2)-[LIVM]-A-x(2)-[LIVMF]-x(2)-[HS]-x-S-T-[LIVM]-S-R. NAME. Sιgma-54 factors family signature 2. CONSENSUS: R-R-T-[IV]-[AT]-K-Y-R. NAME: Sιgma-54 factors family profile. NAME: Sιgma-70 factors family signature 1. CONSENSUS: [DE]-[LIVMF](2)-[HEQS]-x-G-x-[LIVMFA]-G-L-[LIVMFYE]-x-[GSAM]-[LIVMAP]. NAME: Sιgma-70 factors family signature 2 CONSENSUS: [STN]-x(2)-[DEQ]-[LIVM]-[GAS]-x(4)-[LIVMF]-[PSTG]-x(3)-rLIVMA]-x-[NQR]- CONSENSUS [LIVMA]-[EQH]-x(3)-[LIVMFW]-x(2)-[LIVM] NAME Sιgma-70 factors ECF subfamily signature CONSENSUS [STAIV]-[PQDEL]-[DE]-[LIV]-[LIVTA]-Q-x-[STAV]-[LIVMFYC]-[LIVMAK]-x- CONSENSUS [GSTAIV]-[LIMFYWQ]-x(12,14)-[STAP]-[FYW]-[LIF]-x(2)-[IV] NAME Sιgma-54 interaction domain ATP-binding region A signature CONSENSUS [LIVMFY](3)-x-G-[DEQ]-[STE]-G-[STAV]-G-K-x(2)-[LrVMFY] NAME Sιgma-54 interaction domain ATP-bmding region B signature CONSENSUS [GS]-x-[LIVMF]-x(2)-A-[DNEQASH]-[GNEK]-G-[STIM]-[LIVMFY](3)-[DE]-[E ]- CONSENSUS [LIVM] NAME Sιgma-54 interaction domain C-terminal part signature CONSENSUS [FY ]-P-[GS]-N-[LIVM]-R-[EQ]-L-x-[NHAT] NAME Sιgma-54 interaction domain profile NAME Single-strand binding protein family signature 1 CONSENSUS [LIVMF]-[NST]-[KRT]-[LIVM]-x-[LIVMF](2)-G-[NHRK]-[LrVM]-[GST]-x-[DET] NAME Single-strand binding protein family signature 2 CONSENSUS T-x-W-[HY]-[RNS]-[LIVM]-x-[LIVMF]-[FY]-[NGKR] NAME Bacterial histone- ke DNA-binding proteins signature CONSENSUS [GSK]-F-x(2) [LIVMF]-x(4)-[RKEQA]-x(2)-[RST]-x-[GA]-x-[KN]-P-x-T NAME Dps protein family signature 1 CONSENSUS H-[FW]-x-[LIVM]-x-G-x(5)-[LV]-H-x(3)-[DE] NAME Dps protein family signature 2 CONSENSUS [LIVMFY]-[DH]-x-[LIVM]-[GA]-E-R-x(3)-[LIF)-[GDN]-x(2)-[PA] NAME DNA repair protein radC family signature CONSENSUS H-N-H-P-S-G NAME recA signature CONSENSUS A-L-[KR]-[IF]-[FY]-[STA]-[STAD]-[LIVMQ]-R NAME RecF protein signature 1 CONSENSUS P-[ED]-x(3)-[LIVM](2)-x-G-[GSAD]-P-x(2)-R-R-x-[FY]-[LIVM]-D NAME RecF protein signature 2 CONSENSUS [LIVMFY](2)-x-D-x(2,3)-[SA]-[EH]-L-D-x(2)-[KRH]-x(3)-L NAME RecR protein signature CONSENSUS C-x(2)-C-x(3)-[ST]-x(4)-C-x-I-C-x(4)-R NAME Histone H2A signature CONSENSUS [AC]-G-L-x-F-P-V NAME Histone H2B signature CONSENSUS [KR]-E-[LIVM]-[EQ]-T-x(2)-[KR]-x-[LIVM](2)-x-[PAG]-[DE]-L-x-[KR]-H-A- CONSENSUS [LIVM]-[STA]-E-G NAME Histone H3 signature 1 CONSENSUS K-A-P-R-K-Q-L NAME Histone H3 signature 2 CONSENSUS P-F-x-[RA]-L-[VA]-[KRQJ-[DEG]-[IV] NAME Histone H4 signature CONSENSUS G-A-K-R-H NAME HMG1/2 signature CONSENSUS [FI]-S-[KR]-K-C-S-[EK]-R-W-K-T-M NAME HMG-I and HMG-Y DNA-binding domain (A + T-hook) CONSENSUS [AT]-x(l,2)-[RK](2)-[GP]-R-G-R-P-[RK]-x NAME HMG14 and HMG17 signature CONSENSUS R-R-S-A-R-L-S-A-[RK]-P NAME Bromodomam signature CONSENSUS [STANVF]-x(2)-F-x(4)-[DNS]-x(5,7)-[DENQTF]-Y-[HFY]-x(2)-[LIVMFY]-x(3)- CONSENSUS [LIVM]-x(4)-[LIVM]-x(6,8)-Y-x(12,13)-[LIVM]-x(2)-N-[SACF]-x(2)-[FY] NAME Bromodomain profile NAME Chromo domain signature CONSENSUS tFYL]-x-[LIVMC]-[KR]- -x-[GDNR]-[FYWLE]-x(5,6)-[ST]-W-[ES]-[PSTDN]-x(3)- CONSENSUS [LIVMC] NAME Chromo and chromo shadow domain profile NAME Regulator of chromosome condensation (RCCl) signature 1 CONSENSUS G-x-N-D-x(2)-[AV]-L-G-R-x-T NAME Regulator of chromosome condensation (RCCl) signature 2 CONSENSUS [LIVMFA]-[STAGC}(2)-G-x(2)-H-[STAGLI]-[LIVMFA]-x-[LIVM] NAME Protamine PI signature CONSENSUS [AV]-R-[NFY]-R-x(2,3)-[ST]-x-S-x-S NAME Nuclear transition protein 1 signature CONSENSUS S-K-R-K-Y-R-K NAME Nuclear transition protein 2 signature 1 CONSENSUS H-x(3)-H-S-[NS]-S-x-P-Q-S NAME Nuclear transition protein 2 signature 2 CONSENSUS K-x-R-K-x(2)-E-G-K-x(2)-K-[KR]-K NAME Ribosomal protein LI signature CONSENSUS [IM]-x(2)-[LIVA]-x(2,3)-[LIVM}-G-x(2)-[LMS]-[GSNH]-tPTKR]-[KRAV]-G-x- CONSENSUS [LMF]-P-[DENSTK] NAME Ribosomal protein L2 signature CONSENSUS P-x(2)-R-G-[STAIV](2)-x-N-[APK]-x [DE] NAME Ribosomal protein L3 signature CONSENSUS [FL]-x(6)-[DN]-x(2)-[AGS]-x-[ST]-x-G-[KRH]-G-x(2)-G-x(3)-R NAME Ribosomal protein L5 signature CONSENSUS [LrVM]-x(2)-[LIVM]-[STAC]-[GE]-[QV]-x(2)-[LIVMA]-x-[STC]-x-[STAG]-[KR]- CONSENSUS x-[STA] NAME Ribosomal protein L6 signature 1 CONSENSUS [PS]-[DENSl-x-Y-K-[GA]-K-G-[LIVM] NAME Ribosomal protein L6 signature 2 CONSENSUS Q-x(3)-[LIVM]-x(2)-[KR]-x(2)-R-x-F-x-D-G-[LrVM]-Y-[LIVM]-x(2)-[KR] NAME Ribosomal protein L9 signature CONSENSUS G-x(2)-[GN]-x(4)-V-x(2)-G-[FY]-x(2)-N-[FY]-L-x(5)-[GA]-x(3)-[STN] NAME Ribosomal protein L10 signature CONSENSUS [DEH]-x(2)-[GS]-[LIVMF]-[STN]-[VA]-x-[DEQK]-[LIVMA]-x(2)-[LIM]-R NAME Ribosomal protein LI 1 signature CONSENSUS [RKN]-x-[LIVM]-x-G-[ST]-x(2)-[SNQ]-[LIVM]-G-x(2)-[LIVM]-x(0,l)-[DENG] NAME Ribosomal protein L13 signature CONSENSUS [LIVM]-[KRV]-[GK]-M-[LIV]-[PS]-x(4,5)-[GS]-[NQEKRA]-x(5)-[LIVM]-x-[AIV]- CONSENSUS [LFY]-x-[GDN] NAME Ribosomal protein L14 signature CONSENSUS [GA]-[LIV](3)-x(9,10)-[DNS]-G-x(4)-[FY]-x(2)-[NT]-x(2)-V-[LIV] NAME Ribosomal protein LI 5 signature CONSENSUS K-[LIVM](2)-[GAL]-x-[GT]-x-[LIVMA]-x(2,5)-[LIVM]-x-[LIVMF]-x(3,4)- CONSENSUS [LIVMFC]-[ST]-x(2)-A-x(3)-[LIVM]-x(3)-G NAME Ribosomal protein L16 signature 1 CONSENSUS [KR]-R-x-[GSAC]-[KQVA]-[LIVM]-W-[LIVM]-[KR]-[LrVM]-[LFY]-[AP] NAME Ribosomal protein L16 signature 2 CONSENSUS R-M-G-x-[GR]-K-G-x(4)-[FWKR] NAME Ribosomal protein L17 signature CONSENSUS I-x-[ST]-[GT]-x(2)-[KR]-x-K-x(6)-[DE]-x-[LIMV]-[LIVMT]-T-x-[STAG]-[KR] NAME Ribosomal protein L19 signature CONSENSUS [RT]-[ RSVY]-[GSA]-x-V-[RS]-[KR]-[SA]-K-L-Y-Y-L-R NAME Ribosomal protein L20 signature CONSENSUS K-x(3)-[KRC]-x-[LIVM]-W-[IV]-[STNALV]-R-[LIVM]-N-x(3)-[RKH] NAME Ribosomal protein L21 signature CONSENSUS [rVT]-x(3)-[KR]-x(3)-[KRQ]-K-x(6)-G-[HF]-R-[RQ]-x(2)-T NAME Ribosomal protein L22 signature CONSENSUS [RKQN]-x(4)-[RH]-[GAS]-x-G-[KRQS]-x(9)-[HDN]-[LIVM]-x-[LIVMS]-x-[LIVM] NAME Ribosomal protein L23 signature CONSENSUS [RK](2)-[AM]-[IVFYT]-[IV]-[RKT]-L-[STANQK]-x(7)-[LIVMFT] NAME Ribosomal protein L24 signature CONSENSUS [GDEN]-D-X-V-X-[IV]-[LIVMA]-X-G-X(2) [KA]-[GN]-X(2,3)-[GA]-X-[IV] NAME Ribosomal protein L27 signature CONSENSUS G-x-[LIVM](2)-x-R-Q-R-G-x(5)-G Ribosomal protein L29 signature CONSENSUS [KNQS]-[PSTL]-X(2)-[LIMFA]-[KRGSAN]-X-[LIVYSTA]-[KR]-[KRH]-[DESTANRL]- CONSENSUS [LΓV]-A-[KRCQVT]-[LIVMA] NAME Ribosomal protein L30 signature CONSENSUS [rVT]-[LIVM]-x(2)-[LF]-x-[LI]-x-[KRHQEG]-x(2)-[STNQH]-x [IVT]- CONSENSUS x(10)-[LMS]-[LIV]-x(2)-[LrVA}-x(2)-[LMFY]-[IVT] NAME Ribosomal protein L31 signature CONSENSUS H-P-F-[FY]-[TI]-x(9)-G-R-[AV]-x-[KR] NAME Ribosomal protein L33 signature CONSENSUS Y-x-[ST]-x-[KR]-[NS]-x(4) [PAT]-x(l ,2)-[LIVM]-[EA]-x(2)-K-[FY]-[CSD] NAME Ribosomal protein L34 signature CONSENSUS K-[RG]-T [FYWL]-[EQS]-x(5)-[KRHS]-x(4,5)-G-F-x(2)-R NAME Ribosomal protein L35 signature CONSENSUS [LIVM]-K [TV]-x(2)-[GSA]-[SAIL]-x-K-R-[LIVMFY]-[KRL] NAME Ribosomal protein L36 signature CONSENSUS C-x(2)-C-x(2)-[LIVM]-x-R-x(3)-[LrVMN]-x-[LIVM]-x-C-x(3,4)-[KR]-H-x-Q-x-Q NAME Ribosomal protein Lie signature CONSENSUS N-x(3)-[KR]-x(2)-A-[LIVT]-x-S-A-[LIV]-x A-[ST]-[SGA]-x(7)-[RKl-G-H NAME Ribosomal protein L6e signature CONSENSUS N-x(2)-P-L-R-R-x(4)-[FY]-V-I-A-T-S-x-K NAME Ribosomal protein L7Ae signature CONSENSUS [CA]-x(4)-[IV]-P-[FY]-x(2)-[LIVM]-x-[GSQ]-[KRQ]-x(2)-L-G NAME Ribosomal protein LlOe signature CONSENSUS R-x-A-[FYW]-G-K-[PA]-x-G-x(2)-A-R-V NAME Ribosomal protein L13e signature CONSENSUS [KR]-Y-x(2)-K-[LIVM]-R-[STA]-G-[KR]-G-F-[ST]-L-x-E NAME Ribosomal protein L15e signature CONSENSUS tDE]-[KR]-A-R-x-L-G-[FY]-x-[SAP]-x(2)-G-[LIVMFY](4)-R-x-R-V-x-R-G NAME Ribosomal protein L18e signature CONSENSUS [KRE]-x-L-x(2)-[PS]-[KR]-x(2)-[RH]-[PSA]-x-[LIVM]-[NS]-[LrVM]-x-[RK]- CONSENSUS [LIVM] NAME Ribosomal protein L19e signature CONSENSUS R-x-[KR]-x(5)-[KR]-x(3)-[KRH]-x(2)-G-x-G-x-R-x-G-x(3)-A-R-x(3)-[KQ]- CONSENSUS x(2)-W-x(7)-R-x(2)-L-x(3)-R NAME Ribosomal protein L21e signature CONSENSUS G-[DE]-x-V-x(10)-[GV]-x(2)-[FYH]-x(2)-[FY]-x-G-x-T-G NAME Ribosomal protein L24e signature CONSENSUS [FY]-x-[GS]-x(2)-[IV]-x-P-G-x-G-x(2)-[FYV]-x-[KRHE]-x-D NAME Ribosomal protein L27e signature CONSENSUS G-K-N-x-W-F-F-x-K-L-R-F > NAME Ribosomal protein L30e signature 1 CONSENSUS [STA]-x(5)-G-x-[QKR]-x(2)-[LIVM]-[KQT]-x(2)-[KR]-x-G-x(2)-K-x-[LIVM](3) NAME Ribosomal protein L30e signature 2 CONSENSUS [DE]-L-G-[STA]-x(2)-G-[KR]-x(6)-[LIVM]-x-[LIVM]-x-[DEN]-x-G NAME Ribosomal protein L31e signature CONSENSUS V-[KR]-[LIVM]-x(3)-[LIVM]-N-x-[AK]-x-W-x-[KR]-G NAME Ribosomal protein L32e signature CONSENSUS F-x-R-x(4) [KR]-x(2)-[KR]-[LIVM]-x(3)-W-R-[KR]-x(2)-G NAME Ribosomal protein L34e signature CONSENSUS Y-x-[ST]-x-S-[NY]-x(5)-[KR]-T-P-G NAME Ribosomal protein L35Ae signature CONSENSUS G-K-[LIVM]-x-R-x-H-G-x(2)-G-x-V-x-A-x-F-x(3)-[LI]-P Ribosomal protein L36e signature CONSENSUS P-Y-E-[KR]-R-x-[LIVM]-[DE]-[LIVM](2)-[KR] NAME Ribosomal protein L37e signature CONSENSUS G-T-x-[SA]-x-G-x-[KR]-x(3)-[ST]-x(0,l)-H-x(2)-C-x-R-C-G NAME Ribosomal protein L39e signature CONSENSUS [KRA]-T-x(3)-[LIVM]-[KRQF]-x-[NHS]-x(3)-R-[NHY]-W-R-R NAME Ribosomal protein L44e signature CONSENSUS K-x-[TV]-K-K-x(2)-L-[KR]-x(2)-C NAME Ribosomal protein S2 signature 1 CONSENSUS [LIVMFA]-x(2)-[LIVMFYC](2)-x-[STAC]-[GSTANQEKR]-[STALV]-[HY]-[LrVMF]-G NAME Ribosomal protein S2 signature 2 CONSENSUS P-X(2)-[LIVMF](2)-[LΓVMS]-X-[GDN]-X(3)-[DENL]-X(3)-[LIVM]-X-E-X(4)- CONSENSUS [GNQKRH]-[LIVM]-[AP] NAME Ribosomal protein S3 signature CONSENSUS [GSTA]-[KR]-x(6)-G-x-[LIVMT]-x(2)-[NQSCH]-x(l,3)-[LIVFCA]-x(3)-[LIVl- CONSENSUS [DENQ]-x(7)-[LMT]-x(2)-G-x(2)-G NAME Ribosomal protein S4 signawre CONSENSUS [LIVM]-[DE]-x-R-L-x(3)-[LIVMC]-[VMFYHQ]-[KRT]-x(3)-[STAGCF]-x-[ST]-x(3)- CONSENSUS [SAI]-[KR]-x-[LIVMF](2) NAME Ribosomal protein S5 signature CONSENSUS G-[KRQ]-x(3)-[FY]-x-[ACV]-x(2)-[LIVMA]-[LIVM]-[AG]-[DN]-x(2)-G-x- CONSENSUS [LrVM]-G-x-[SAG]-x(5,6)-[DEQ]-[LIVM]-x(2)-A-[LIVMF] NAME Ribosomal protein S6 signature CONSENSUS G-x-[KRC]-[DENQRH]-L-[SA]-Y-x-I-[KRNSA] NAME Ribosomal protein S7 signature CONSENSUS [DENSK]-x-[LIVMET]-x(3)-[LrVMFT](2)-x(6)-G-K-tKR]-x(5)-[LIVMF]-[LIVMFC]- CONSENSUS x(2)-[STA] NAME Ribosomal protein S8 signature CONSENSUS [GE]-x(2)-[LIV](2)-[STY]-T-x(2)-G-[LIVM](2)-x(4)-[AG]-[KRHAYI] NAME Ribosomal protein S9 signature CONSENSUS G-G-G-x(2)-[GSA]-Q-x(2)-[SA]-x(3)-[GSA]-x-[GSTAV]-[KR]-[GSAL]-[LIF] NAME Ribosomal protein S10 signature CONSENSUS [AV]-x(3)-[GDNSR]-[LIVMSTA]-x(3)-G-P-[LIVM]-x-[LIVM]-P-T NAME Ribosomal protein Sl l signature CONSENSUS [LP MF]-x-[GSTAC]-[LIVMF]-x(2)-[GSTAL]-x(0, l)-[GSN]-[LIVMF]-x-[LIVM]- CONSENSUS x(4)-[DEN]-x-T-P-x-[PA]-[STCH]-[DN] NAME Ribosomal protein S12 signature CONSENSUS [RK]-x-P-N-S-[AR]-x-R NAME Ribosomal protein S13 signature CONSENSUS [KRQS]-G-x-R-H-x(2)-[GSNH}-x(2)-[LIVMC]-R-G-Q NAME Ribosomal protein S14 signature CONSENSUS [RP]-x(0,l)-C-x(l l,12)-[LIVMF]-x-[LIVMF]-[SC]-[RG]-x(3)-[RN] NAME Ribosomal protein S15 signature CONSENSUS [LrVM]-x(2)-H-[LIVMFY]-x(5)-D-x(2)-[SAGN]-x(3)-[LF]-x(9)-[LIVM]-x(2)- CONSENSUS [FY] NAME Ribosomal protein S16 signature CONSENSUS [LIVMT]-x-[LIVM]-[KR]-L-[STAK]-R-x-G-[AKR] NAME Ribosomal protein S17 signature CONSENSUS G-D-x-[LIV]-x-[LIVA]-x-[QEK]-x-[RK]-P-[LIV]-S NAME Ribosomal protein S18 signature CONSENSUS [IV]-[DY]-Y-x(2) [LIVMT]-x(2)-[LIVM]-x(2) [FYT]-[LIVM]-[ST]-[DERP]-x- CONSENSUS [GY]-K-[LIVM]-x(3)-R-[LIVMAS] NAME Ribosomal protein S19 signature CONSENSUS [STDNQ]-G-[KRQM]-x(6)-[LIVM]-x(4)-[LIVM]-[GSD]-x(2)-[LF]-[GAS]-[DE]-F- CONSENSUS x(2)-[ST] NAME Ribosomal protein S21 signature CONSENSUS [DE]-x-A-[LY]-[KR]-R-F-K-[KR]-x(3)-[KR] NAME Ribosomal protein S3Ae signature CONSENSUS [LIV]-x-[GH]-R-[rV]-x-E-x-[SC]-L-x-D-L NAME Ribosomal protein S4e signature CONSENSUS H-x-K-R-[LIVM]-[SAN]-x-P-x(2)-W-x-[LIVM]-x-[KR] NAME Ribosomal protein S6e signature CONSENSUS [LIVM] [STAMR]-G-G-x-D-x(2)-G-x-P-M NAME Ribosomal protein S7e signature CONSENSUS [KR]-L-x-R-E-L-E-K-K-F-[SAP]-x-[KR]-H NAME Ribosomal protein S8e signature CONSENSUS R-x(2)-T-G-[GA]-x(5)-[HR]-K [KR]-x-K-x-E-[LM]-G NAME Ribosomal protein S12e signature CONSENSUS A-L-[KRQP]-x-V-L-x(2)-[SA]-x(3)-[DN]-G-L NAME Ribosomal protein S17e signature CONSENSUS A-x-I-x-[ST]-K-x-L-R-N-[KR]-I-A-G-[FY]-x-T-H NAME Ribosomal protein S19e signature CONSENSUS P-x(6)-[SAN]-x(2)-[LIVMA]-x-R-x-[ALIV]-[LV]-Q-x-L-[EQ] NAME Ribosomal protein S21e signature CONSENSUS L-Y-V-P-R-K-C-S-[SA] NAME Ribosomal protein S24e signature CONSENSUS [FA]-G-x(2)-[KR]-[STA]-x-G-[FY]-[GA]-x-[LIVM]-Y-[DN]-[SN] NAME Ribosomal protein S26e signature CONSENSUS [YH]-C-V-S-C-A-I-H NAME Ribosomal protein S27e signature CONSENSUS [QK]-C-x(2)-C-x(6)-F-[GS]-x-[PSA]-x(5)-C-x(2)-C-[GS]-x(2)-L-x(2)-P-x-G NAME Ribosomal protein S28e signature CONSENSUS E-[ST]-E-R-E-A-R-x-L NAME DNA mismatch repair proteins mutL / hexB / PMS1 signature CONSENSUS G-F-R-G-E-A-L NAME DNA mismatch repair proteins mutS family signature CONSENSUS [ST]-[LIVM]-x-[LIVM]-x-D-E-[LIVMY]-[GC]-[RKH]-G-[GST]-x(4)-G NAME muiT domain signature CONSENSUS G-x(5)-E-x(4)-[STAGCJ-[LIVMAC]-x-R-E-[LIVMFT]-x-E-E NAME DnaA protein signature CONSENSUS I-[GA]-x(2)-[LIVMF]-[SGDNK]-x(0,l)-[KR]-x-H-[STP]-[STV]-[LIVM](2)-x- CONSENSUS [SA]-x(2)-[KRE]-[LIVM] NAME Small, acid-soluble spore proteins, alpha/beta type, signature 1 CONSENSUS K-x-E-[LIV]-A-x-[DE]-[LIVMF]-G-[LIVMFl NAME Small, acid-soluble spore proteins, alpha/beta type, signature 2 CONSENSUS [KR]-[SAQ]-x-G-x-V-G-G-x-[LIVM]-x-[KR](2)-[LIVM](2) NAME Zinc-containing alcohol dehydrogenases signature CONSENSUS G-H-E-x(2)-G-x(5)-[GA]-x(2)-[IVSAC] NAME Qumone oxidoreductase / zeta-crystal n signature CONSENSUS [GSD]-[DEQH]-x(2)-L-x(3)-[SA](2)-G-G-x-G-x(4)-Q-x(2)-[KR] NAME Iron-containing alcohol dehydrogenases signature 1 CONSENSUS [STALIV]-[LIVF]-x-[DE]-x(6,7)-P-x(4)-[ALrV]-x-[GST]-x(2)-D-[TAIVM]- CONSENSUS [LIVMF]-x(4)-E NAME Iron-containing alcohol dehydrogenases signature 2 CONSENSUS [GSW]-x-[LIVTSACD]-[GH]-x(2)-[GSAE]-[GSHYQ]-x-[LrVTPHGAST]-[GAS]-x(3)- CONSENSUS [LIVMT]-x-[HNS]-[GA]-x-[GTAC] NAME Short-chain dehydrogenases/reductases family signature CONSENSUS [LIVSPADNK]-x(12)-Y-[PSTAGNCV]-[STAGNQCIVM]-[STAGC] K-{PC}-[SAGFR]- CONSENSUS [LIVMSTAGD]-x(2)-[LIVMFYW]-x(3)-[LIVMFYWGAPTHQ]-[GSACQRHM] NAME Aldo/keto reductase family signature 1 CONSENSUS G-[FY]-R-[HSAL]-[LIVMF]-D-[STAGC]-[AS]-x(5)-E-x(2)-[LIVM]-G NAME Aldo/keto reductase family signature 2 CONSENSUS [LIVMFY]-x(9)-[KREQ]-x-[LIVM]-G-[LIVM]-[SC]-N-[FY] NAME Aldo/keto reductase family putative active site signature CONSENSUS [LIVM]-[PAIV]-[KR]-[ST]-x(4)-R-x(2)-[GSTAEQK]-[NSL]-x(2)-[LIVMFA] NAME Homoseπne dehydrogenase signature CONSENSUS A-x(3)-G-[LrVMFY]-[STAG]-x(2,3)-[DNS] P-x(2)-D-[LIVM]-x-G-x-D-x(3)-K NAME NAD-dependent glycerol-3-phosphate dehydrogenase signature CONSENSUS G-[AT]-[LIVM]-K-[DN]-[LIVM](2)-A-x-[GA]-x-G-[LIVMF]-x-[DE]-G-[LIVM]-x- CONSENSUS [LIVMFYW]-G-x-N NAME FAD-dependent glycerol-3-phosphate dehydrogenase signature 1 CONSENSUS [IV]-G-G-G-x(2)-G-[STACV]-G-x-A-x-D-x(3)-R-G NAME FAD-dependent glycerol-3-phosphate dehydrogenase signature 2 CONSENSUS G-G-K-x(2)-[GSTE]-Y-R-x(2)-A NAME Mannitol dehydrogenases signature CONSENSUS [LIVMY]-x-[FS]-x(2)-[STAGCV]-x-V-D-R-[IV]-x-[PS] NAME Histidinol dehydrogenase signature CONSENSUS I-D-x(2)-A-G-P-[ST]-E-[LIVS]-[LIVMA](3)-[AC]-x(3)-A-x(4)-[LIVM]-[AV]- CONSENSUS [SACL]-[DE]-[LIVMFC]-[LIVM]-[SA]-x(2)-E-H NAME L-lactate dehydrogenase active site CONSENSUS [LIVMA]-G-[EQ]-H-G-[DN]-[ST] NAME D-isomer specific 2-hydroxyacid dehydrogenases NAD-binding signature CONSENSUS [LIVMA]-[AG]-[IVT]-[LIVMFY]-[AG]-x-G-[NHKRQGSAC]-[LrV]-G-x(13,14)- CONSENSUS [LIVfMT]-x(2)-[FYwCTH]-[DNSTK] NAME D-isomer specific 2-hydroxyacid dehydrogenases signature 2 CONSENSUS [LIVMFYWA]-[LIVFYWC]-x(2)-[SAC]-[DNQHR]-[IVFA]-[LIVF]-x-[LIVF]-[HNri-x- CONSENSUS P-x(4)-[STN]-x(2)-[LIVMF]-x-[GSDN] NAME D-isomer specific 2-hydroxyacid dehydrogenases signature 3 CONSENSUS [LMFATC]-[KPQ]-x-[GSTDN]-x-[LIVMFYWR]-[LIVMFY ](2)-N-x-[STAGC]-R-[GP]-x- CONSENSUS [LIVH]-[LIVMC]-[DNV] NAME 3-hydroxyιsobutyrate dehydrogenase signature CONSENSUS [LIVMFY](2)-G-L-G-x-[MQ]-G-x-[PGS]-[MA]-[SA] NAME Hydroxymethylglutaryl-coenzyme A reductases signature 1 CONSENSUS [RKH]-x(6)-D-x-M-G-x-N-x-[LIVMA] NAME Hydroxymethylglutaryl-coenzyme A reductases signature 2 CONSENSUS [LIVM]-G-x-[LIVM]-G-G-[AG]-T NAME Hydroxymethylglutaryl-coenzyme A reductases signature 3 CONSENSUS A-[LIVM]-x-[STAN]-x(2)-[LI]-x-[KRNQ]-[GSA]-H-[LM]-x-[FYLH] NAME Hydroxymethylglutaryl-coenzyme A reductases profile NAME 3-hydroxyacyl-CoA dehydrogenase signature CONSENSUS [DNE]-x(2)-[GA]-F-[LIVMFY]-x-[NTJ-R-x(3)-[PA]-[LIVMFY](2)-x(5)- CONSENSUS [LIVMFYCTHLIVMFY]-x(2MGV] NAME Malate dehydrogenase active site signature CONSENSUS [LIVM]-T-[TRKMN]-L-D-x(2)-R-[STA]-x(3)-[LIVMFY] NAME Malic enzymes signature CONSENSUS F-x-[DV]-D-x(2)-G-T-[GSA]-x-[IV]-x-[LIVMA]-[GAST](2)-[LIVMF](2) NAME Isocitrate and isopropylmalate dehydrogenases signature CONSENSUS [NS]-[LIMYT]-[FYDN]-G-[DNT]-[IMVY]-x-[STGDN]-[DN]-x(2)-[SGAP]-x(3,4)-G- CONSENSUS [STG]-[LIVMPA]-G-[LIVMF] NAME 6-phosphogluconate dehydrogenase signature CONSENSUS [LIVM]-x-D-x(2)-[GA]-[NQS]-K-G-T-G-x-W NAME Glucose-6-phosphate dehydrogenase active site CONSENSUS D-H-Y-L-G-K-[EQK] NAME IMP dehydrogenase / GMP reductase signature CONSENSUS [LIVM]-[RK] [LIVM]-G-[LIVM]-G-x G S-[LIVM]-C-x-T NAME Bactenal quinoprotein dehydrogenases signature 1 CONSENSUS [DEN]-W-x(3)-G-[RK]-x(6)-[FYW]-S-x(4)-[LIVM]-N-x(2)-N-V-x(2)-L-[RK] NAME Bacterial quinoprotein dehydrogenases signature 2 CONSENSUS W-x(4)-Y-D-x(3)-[DN]-[LrVMFY](4)-x(2)-G-x(2)-[STA]-P NAME FMN-dependent alpha-hydroxy acid dehydrogenases active site CONSENSUS S-N-H-G-[AG]-R-Q NAME GMC oxidoreductases signature 1 CONSENSUS [GA]-[RKN]-x-[LIV]-G(2)-[GST](2)-x-[LIVM}-N-x(3)-[FYWA]-x(2)-[PAG]-x(5)- CONSENSUS [DNESH] NAME GMC oxidoreductases signature 2 CONSENSUS [GS]-[PSTA]-x(2)-[ST]-P-x-[LIVM](2)-x(2)-S-G-[LIVM]-G NAME Eukaryotic molybdopteπn oxidoreductases signature CONSENSUS [GA]-x(3)-[KRNQHT]-x(l l,14)-[LIVMFYWS]-x(8)-[LIVMF]-x-C-x(2)-[DEN]-R- CONSENSUS x(2)-[DE] NAME Prokaryotic molybdoptenn oxidoreductases signature 1 CONSENSUS [STAN]-x-[CH]-x(2,3)-C-[STAG]-[GSTVMF]-x-C-x-[LIVMFY ]-x-[LIVMA]-x(3,4)- CONSENSUS [DENQKHT] NAME Prokaryotic molybdoptenn oxidoreductases signature 2 CONSENSUS [STA]-x-[STAC](2)-x(2)-[STA]-D-[LIVMY](2)-L-P-x-[STAC](2)-x(2)-E NAME Prokaryotic molybdoptenn oxidoreductases signature 3 CONSENSUS A-x(3)-[GDT]-I-x-[DNQTK]-x-[DEA]-x-[LIVM]-x-[LIVMC]-x-[NS]-x(2)-[GS]- CONSENSUS x(5)-A-x-[LIVM]-[ST] NAME Aldehyde dehydrogenases glutamic acid active site CONSENSUS [LIVMFGA]-E-[LIMSTAC]-[GS]-G-[KNLM]-[SADN]-[TAPFV] NAME Aldehyde dehydrogenases cysteine active site CONSENSUS [FYLVA]-x(3)-G-[QE]-x-C-[LIVMGSTANC]-[AGCN]-x-[GSTADNEKR] NAME Aspartate-semialdehyde dehydrogenase signature CONSENSUS [LIVM]-[SADN]-x(2)-C-x-R-[LIVM]-x(4)-[GSC]-H-[STA] NAME Glyceraldehyde 3-phosphate dehydrogenase active site CONSENSUS [ASV]-S-C-[NT]-T-x(2)-[LIM] NAME N-acetyl-gamma-glutamyl-phosphate reductase active site CONSENSUS [LIVM]-[GSA]-x-P-G-C-[FY]-[AVP]-T-[GA]-x(3)-[GTAC]-[LIVM]-x-P NAME Gamma-glutamyl phosphate reductase signamre CONSENSUS V-x(5)-A-[LIV]-x-H-I-x(2)-[HY]-tGS]-[ST]-x-H-tST]-tDE]-x-I NAME Dihydrodipico nate reductase signature CONSENSUS E-[IV]-x-E-x-H-x(3)-K-x-D-x-P-S-G-T-A NAME Dihydroorotate dehydrogenase signature 1 CONSENSUS [GS]-x(4)-[GK]-[STA]-[IVSTA]-[GT]-x(3)-[NQR]-x-G-[NH]-x(2)-P-[RT] NAME Dihydroorotate dehydrogenase signamre 2 CONSENSUS [LIV](2)-[GSA]-x-G-G-[IV]-x-[STGN]-x(3)-[ACV]-x(6)-G-A NAME Coproporphyπnogen III oxidase signature CONSENSUS K-x-W-C-x(2)-[FYH](3)-[LIVM]-x-H-R-x-E-x-R-G-[LIVM]-G-G-[LIVM]-F-F-D NAME Fumarate reductase / succmate dehydrogenase FAD-binding site CONSENSUS R-[ST]-H-[ST]-x(2)-A-x-G-G NAME Acyl-CoA dehydrogenases signature 1 CONSENSUS [GAC]-[LIVM]-[ST]-E-x(2)-[GSAN]-G-[ST]-D-x(2)-[GSA] NAME Acyl-CoA dehydrogenases signature 2 CONSENSUS [QDE]-x(2)-G-[GS]-x-G-[LIVMFY]-x(2)-[DEN]-x(4)-[KR]-x(3)-[DEN] NAME Alamne dehydrogenase & pyridine nucleotide transhydrogenase signamre 1 CONSENSUS G-[LIVM]-P-x-E-x(3)-N-E-x(l,3)-R-V-A-x-[ST]-P-x-[GST]-V-x(2)-L-x-[KRH]- CONSENSUS x-G NAME Alamne dehydrogenase & pyndine nucleotide transhydrogenase signature 2 CONSENSUS [LIVM](2)-G-[GA]-G-x-A-G-x(2)-[SA]-x(3)-[GA]-x-[SG]-[LIVM]-G-A-x-V- CONSENSUS x(3)-D NAME Glu / Leu / Phe / Val dehydrogenases active site CONSENSUS [LIV]-X(2)-G-G-[SAG]-K-X-[GV]-X(3)-[DNST]-[PL] NAME D-amino acid oxidases signature CONSENSUS [LIVM](2)-H-[NHA]-Y-G-x-[GSA](2)-x-G-x(5)-G-x-A NAME Pyπdoxaimne 5 '-phosphate oxidase signamre CONSENSUS [LIVF]-E-F-W-[QHG]-x(4)-R-[LIVM]-H-[DNE]-R NAME Copper amine oxidase topaquinone signature CONSENSUS [LIVM]-[LIVMA]-[LIVM]-x(4)-T-x(2)-N-Y-[DE]-[YN] NAME Copper amine oxidase copper-binding site signature CONSENSUS T-x-G-x(2)-H-[LIVMF]-x(3)-E-[DE]-x-P NAME Lysyl oxidase putative copper-binding region signature CONSENSUS W-E-W-H-S-C-H-Q-H-Y-H NAME Delta l-pyrrohne-5-carboxylate reductase signamre CONSENSUS [PALF]-x(2,3)-[LIV]-x(3)-[LIVM]-[STAC]-[STV]-x-[GAN]-G-x-T-x(2)-[AG]- CONSENSUS [LIV]-x(2)-[LMF]-[DENQK] NAME Dihydrofolate reductase signamre CONSENSUS [LVAGC]-[LIF]-G-x(4)-[LIVMF]-P-W-x(4,5)-[DE]-x(3)-[FYIV]-x(3)-[SΗQ] NAME Tetrahydrofolate dehydrogenase/cyclohydrolase signature 1 CONSENSUS [EQ]-X-[EQK]-[LIVM](2)-X(2)-[LΓVM]-X(2)-[LΓVMY]-N-X-[DN]-X(5)-[LΓVMF](3)- CONSENSUS: Q-L-P-[LV]. NAME: Tetrahydrofolate dehydrogenase/cyclohydrolase signature 2 CONSENSUS P-G-G-V-G-P-[MF]-T-[IV], NAME- Oxygen oxidoreductases covalent FAD-bmding site. CONSENSUS' P-X(10)-[DE]-[LIVM]-X(3)-[LIVM]-X(9)-[LIVM]-X(3)-[GSA]-[GST]-G-H. NAME Pyridine nucleotide-disulphide oxidoreductases class-I active site. CONSENSUS- G-G-X-C-[LIVA]-X(2)-G-C-[LIVM]-P. NAME Pyridine nucleotide-disulphide oxidoreductases class-II active site CONSENSUS: C-X(2)-C-D-[GA]-X(2,4)-[FY]-X(4)-[LIVM]-X-[LIVM](2)-G(3)-[DN] NAME Respiratory-chain NADH dehydrogenase subunit 1 signature 1 CONSENSUS. G-[LIVMFY RS]-[LIVMAGP]-Q-X-[LIVMFY]-X-D-[AGIM]-[LIVMFTA]-K-[LVMYST]- CONSENSUS [LIVMFYG]-x-[KR]-[EQG] NAME Respiratory-chain NADH dehydrogenase subumt 1 signature 2 CONSENSUS: P-F-D-[LIVMFYQ]-[STAGPVM]-E-[GAC]-E-x-[EQ]-[LIVMS]-x(2)-G NAME Respiratory-cham NADH dehydrogenase 20 Kd subumt signature. CONSENSUS- [GN]-x-D-[KRST]-[LIVMF](2)-P-[IV]-D-[LIVMFYW](2)-x-P-x-C-P-[PT]. NAME Respiratory-cham NADH dehydrogenase 24 Kd subumt signature. CONSENSUS ■ D-x(2)-F-[ST]-x(5)-C-L-G-x-C-x(2)-[GA]-P NAME Respiratory chain NADH dehydrogenase 30 Kd subunit signature. CONSENSUS. E-R-E-X(2)-[DE]-[LΓVMF](2)-X(6)-[HK]-X(3)-[KRP]-X-[LIVM]-[LIVMS]. NAME Respiratory chain NADH dehydrogenase 49 Kd subunit signamre. CONSENSUS [LIVMH]-H-[RT]-[GA]-x-E-K-[LIVMT]-x-E-x-[KRQ] NAME. Respiratory-cham NADH dehydrogenase 51 Kd subumt signamre 1 CONSENSUS G-[AM]-G-[AR]-Y-[LIVM]-C-G-[DE](2)-[STA](2)-[LIM](2)-[EN]-S. NAME Respiratory-chain NADH dehydrogenase 51 Kd subunit signamre 2. CONSENSUS. E-S-C-G-x-C-x-P-C-R-x-G. NAME' Respiratory-chain NADH dehydrogenase 75 Kd subunit signamre 1. CONSENSUS- P-x(2)-C-[Y S]-x(7)-G-x-C-R-x-C. NAME Respiratory-cham NADH dehydrogenase 75 Kd subunit signature 2. CONSENSUS C-P-x-C-[DE]-x-[GS](2)-x-C-x-L-Q. NAME. Respiratory-cham NADH dehydrogenase 75 Kd subumt signature 3. CONSENSUS: R-C-[LIVM]-x-C-x-R-C-[LIVM]-x-[FY]. NAME Nitrite and sulfite reductases lron-sulfur/siroheme-binding site CONSENSUS: [STV]-G-C-x(3)-C-x(6)-[DE]-[LIVMF]-[GAT]-[LIVMF]. NAME: Uncase signature CONSENSUS: L-x-[LV]-L-K-[ST]-T-x-S-x-F-x(2)-[FY]-x(4)-[FY] NAME Heme-copper oxidase catalytic subunit, copper B binding region signamre. CONSENSUS: [YWG]-[LIVFYWTA](2)-[VGS]-H-[LNP]-x-V-x(44,47)-H-H. NAME CO II and mtrous oxide reductase dinuclear copper centers signamre. CONSENSUS: V-x-H-x(33,40)-C-x(3)-C-x(3)-H-x(2)-M. NAME: Cytochrome c oxidase subumt Vb, zinc bindmg region signamre. CONSENSUS: [LIVM](2)-[FY ]-x(10)-C-x(2)-C-G-x(2)-[FYl-K-L. NAME Multicopper oxidases signamre 1. CONSENSUS: G-x-[FY ]-x-[LrVMFYW]-x-[CST]-x(8)-G-[LM]-x(3)-[LIVMFYW]. NAME: Multicopper oxidases signamre 2. CONSENSUS: H-C-H-x(3)-H-x(3)-[AG]-[LM] NAME: Peroxidases proximal heme-hgand signature. CONSENSUS. [DET]-[LIVMTA]-x(2)-[LIVM]-[LIVMSTAG]-[SAG]-[LIVMSTAG]-H-[STA]-[LIVMFY]. NAME: Peroxidases active site signamre. CONSENSUS: [SGATV]-x(3)-[LIVMA]-R-[LIVMA]-x-[FW]-H-x-[SAC] . NAME Catalase proximal heme-hgand signamre. CONSENSUS. R-[LIVMFSTAN]-F-[GASTNP]-Y-x-D-[AST]-[QEH] NAME: Catalase proximal active site signamre CONSENSUS [IF]-x-[RH]-x(4)-[EQ]-R-x(2)-H-x(2)-[GAS]-[GASTF]-[GAST]. NAME Glutathione peroxidases selenocysteine active site CONSENSUS: [GN]-[RKHNFYC]-x-[LIVMFC]-[LIVMF](2)-x-N-[VT]-x-[STC]-x-C-[GA]-x-T. NAME: Glutathione peroxidases signature 2 CONSENSUS [LIV]-[AGD]-F-P-[CS]-[NG]-Q-F. NAME: Lipoxygenases iron-binding region signature 1. CONSENSUS. H-[EQ]-x(3)-H-x-[LM]-[NQRC]-[GST]-H-[LIVMSTAC](3)-E NAME Lipoxygenases iron-binding region signature 2. CONSENSUS: [LIVMA]-H-P-[LIVM]-x-[KRQ]-[LIVMF](2)-x-[AP]-H. NAME: Extradiol ring-cleavage dioxygenases signature. CONSENSUS: [GNTIV]-x-H-x(5,7)-[LIVMF]-Y-x(2)-[DENTA]-P-x-[GP]-x(2,3)-E NAME: Intradiol nng -cleavage dioxygenases signamre. CONSENSUS- [LIVM]-x-G-x-[LIVM]-x(4)-[GS]-x(2)-[LIVM]-x(4)-[LIVM]-[DE]-[LIVMFY]- CONSENSUS: x(6)-G-x-[FY]. NAME. Indoleamme 2,3-dιoxygenase signamre 1. CONSENSUS: G-G-S-[AN]-[GA]-Q-S-S-x(2)-Q. Indoleamme 2,3-dιoxygenase signamre 2 CONSENSUS' [FY]-L-[DQ]-[DE]-[LIVM]-x(2)-Y-M-x(3)-H-[KR] NAME Bacterial ring hydroxylatmg dioxygenases alpha-subumt signamre CONSENSUS C-x-H-R-[GA]-x(8)-G-N-x(5)-C-x-[FY]-H. NAME Bacterial luciferase subunits signamre CONSENSUS [GA]-[LIVM]-P-[LIVM]-x-[LIVMFY]-x-W-x(6)-[RK]-x(6)-Y-x(3)-[AR] NAME ubiH/COQ6 monooxygenase family signature CONSENSUS H-P-[LIV]-[AG]-G-Q-G-x-N-x-G-x(2)-D NAME Biopteπn-dependent aromatic amino acid hydroxylases signamre. CONSENSUS P-D-x(2)-H-[DE]-[LI]-[LIVMF]-G-H-[LIVMC]-P NAME Copper type II, ascorbate-dependent monooxygenases signamre 1. CONSENSUS- H-H-M-x(2)-F-x-C. NAME' Copper type II, ascorbate-dependent monooxygenases signamre 2. CONSENSUS : H-x-F-x(4)-H-T-H-x(2)-G NAME: Tyrosinase CuA-binding region signamre CONSENSUS. H-x(4,5)-F-[LIVMFTP]-x-[FW]-H-R-x(2)-[LM]-x(3)-E. NAME: Tyrosinase and hemocyanins CuB-binding region signature. CONSENSUS: D-P-x-F-[LIVMFY ]-x(2)-H-x(3)-D. NAME Fatty acid desaturases family 1 signamre. CONSENSUS G-E-x-[FY] -H-N-[FY]-H-H-x-F-P-x-D-Y NAME: Fatty acid desaturases family 2 signature. CONSENSUS [ST]-[SA]-x(3)-[QR]-[LI]-x(5,6)-D-Y-x(2)-[LIVMFY ]-[LIVM]-[DE]. NAME: Cytochrome P450 cysteine he e-iron ligand signature. CONSENSUS. [FW]-[SGNH]-x-[GD]-x-[RHPT]-x-C-[LIVMFAP]-[GAD] NAME- Heme oxygenase signamre. CONSENSUS: L-L-V-A-H-A-Y-T-R. NAME: Copper/Zinc superoxide dismutase signamre 1. CONSENSUS: [GA]-[IFAT]-H-[LIVF]-H-x(2)-[GP]-[SDG]-x-[STAGD]. NAME: Copper/Zmc superoxide dismutase signamre 2. CONSENSUS: G-[GN]-[SGA]-G-x-R-x-[SGA]-C-x(2)-[IV] . NAME Manganese and iron superoxide dismutases signamre CONSENSUS D-x-W-E-H-[STA]-[FY](2) NAME Ribonucleotide reductase large subumt signamre CONSENSUS W-x(2)-[LF]-x(6,7)-G-[LIVM]-[FYRA]-[NH]-x(3)-[STAQLIVM]-[ASC]-x(2)- CONSENSUS [PA] NAME Ribonucleotide reductase small subunit signamre CONSENSUS [IVMSEQ]-E-x( 1 ,2)-[LIVTA]-[HY]-[GSA]-x-[STAVM]-Y-x(2)-[LIVMQ]-x(3)- CONSENSUS [LIFYHIVFYCSA] NAME Nitrogenases component 1 alpha and beta subunits signamre 1 CONSENSUS [LIVMFYH]-[LIVMFST]-H-[AG]-[AGSP]-[LIVMNQA]-[AG]-C NAME Nitrogenases component 1 alpha and beta subunits signamre 2 CONSENSUS [STANQ]-[ET]-C-x(5)-G-D-[DN]-[LIVMT]-x-[STAGR]-[LIVMFYST] NAME NifH/frxC family signature 1 CONSENSUS E-x-G-G-P-x(2)-[GA]-x-G-C-[AG]-G NAME NifH/frxC family signamre 2 CONSENSUS D-x-L-G-D-V-V-C-G-G-F-[AG]-x-P NAME Nickel-dependent hydrogenases large subumt signamre 1 CONSENSUS R-G-[LIVMF]-E-x(15)-[QESM]-R-x-C-G-[LIVM]-C NAME Nickel-dependent hydrogenases large subumt signamre 2 CONSENSUS [FY]-D-P-C-[LIM]-[ASG]-C-x(2,3)-H NAME Glutamyl-tRNA reductase signamre CONSENSUS H-[LIVM]-x(2)-[LIVM]-[GSTAC](3)-[LIVM]-[DEQ]-S-[LrVMA]-[LIVM](2)-[GF]-E- CONSENSUS x-[QR]-[IV]-[LIT]-[STAG]-Q-[LIVM]-[KR] NAME Bactenal-type phytoene dehydrogenase signamre CONSENSUS [NG]-x-[FYWV]-[LIVMF]-x-G-[AGC]-[GS]-[TA]-[HQT]-P-G-[STAV]-G-[LIVM]- CONSENSUS x(5)-[GS] NAME Glycme radical signamre CONSENSUS [STIV]-x-R-[IVT]-[CSA]-G-Y-x-[GACV] NAME Ergosterol biosynthesis ERG4/ERG24 family signamre 1 CONSENSUS G-x(2)-[LIVM]-Y-D-x-[FY]-x-G-x(2)-L-N-P-R NAME Ergosterol biosynthesis ERG4/ERG24 family signamre 2 CONSENSUS [LIVM] (2)-H-R-x(2)-R-D-x(3)-C-x(2)-K-Y-G NAME NNMT/PNMT/TEMT family of methyltraπsferases signamre CONSENSUS L-I-D-I-G-S-G-P-T-[IV]-Y-Q-L-L-S-A-C NAME RNA methyltransferase trmA family signamre 1 CONSENSUS [DN]-P-[PA]-R-x-G-x(14, 16)-[LIVM](2)-Y-x-S-C-N-x(2)-T NAME RNA methyltransferase trmA family signamre 2 CONSENSUS [LrVMF]-D-x-F-P-[QHY]-[ST]-x-H-[LIVMFY]-E NAME Thymidylate synthase active site CONSENSUS R-x(2)-[LIVM]-x(3)-[FW]-[QN]-x(8,9)-[LV]-x-P-C-[HAVM]-x(3)-[QMT]-[FYW]- CONSENSUS x-[LV] NAME Ribosomal RNA ademne dunethylases signamre CONSENSUS [LIVM]-[LrVMFY]-[DE]-x-G-[STAPV]-G-x-[GA]-x-[LrVMF]-[ST]-x(2)-[LIVM]- CONSENSUS x(6)-[LIVMY]-x-[STAGV]-[LIVMFYHC]-E-x-D NAME Methylated-DNA--proteιn-cysteιne methyltransferase active site CONSENSUS [LrVMF]-P-C-H-R-[LIVMF](2) NAME N-6 Adenine-specific DNA methylases signamre CONSENSUS [LIVMAC]-[LIVFYWA]-x-[DN]-P-P-[FYW] NAME N-4 cytosine-specific DNA methylases signamre CONSENSUS [LIVMF]-T-S-P-P-[FY] NAME C-5 cytosine-specific DNA methylases active site CONSENSUS [DENKS]-x-[FLIV]-x(2)-[GSTC]-x-P-C-x(2)-[FY LIM]-S NAME C-5 cytosine-specific DNA methylases C-terminal signamre CONSENSUS [RKQGTF]-x(2)-G-N-[STAG]-[LIVMF]-x(3)-[LIVMT]-x(3)-[LIVM]-x(3)-[LIVM] NAME Proteιn-L-ιsoaspartate(D-aspartate) O-methyltransferase signamre CONSENSUS [GSA]-D-G-x(2)-G-[FYWV]-x(3)-[AS]-P-[FY]-[DN]-x-I NAME Uropo hyπn-III C-methyltransferase signamre 1 CONSENSUS [LIVM]-[GS]-[STAL]-G-P-G-x(3)-[LIVMFY]-[LIVM]-T-[LIVM]-[KRHQG]-[AG] NAME Uroporphyπn-III C-methyltransferase signamre 2 CONSENSUS V-x(2)-[LI]-x(2)-G-D-x(3)-[FYW]-[GS]-x(8)-[LIVF]-x(5,6)-[LIVMFY PAC]- CONSENSUS x-[LIVMY]-x-P-G NAME ubιE/C0Q5 methyltransferase family signamre 1 CONSENSUS Y-D-x-M-N-x(2)-[LIVM]-S-x(3)-H-x(2)-W NAME ubiE/COQ5 methyltransferase family signamre 2 CONSENSUS R-V-[LIVM]-K-[PV]-G-G-x-[LIVMF]-x(2)-[LIVM]-E-x-S NAME Senne hydroxymethyltransferase pyπdoxal-phosphate attachment site CONSENSUS [DEH]-[LIVMFY]-x-[STMV]-[GST]-[ST](2)-H-K-[ST]-[LF]-x-G-[PAC]-[RQ]- CONSENSUS [GSA]-[GA] NAME Phosphonbosylglycinamide formyltransferase active site CONSENSUS G-x-[STM]-[IVT]-x-[FY VQ]-[VMAT]-x-[DEVM]-x-[LIVMY]-D-x-G-x(2)-[LIVTJ- CONSENSUS x(6)-[LIVM] NAME Aspartate and ornithine carbamoyltransferases signamre CONSENSUS F-x-[EK]-x-S-[GT]-R-T NAME Transketolase signamre 1 CONSENSUS R-x(3)-[LIVMTA]-[DENQSTHKF]-x(5,6)-[GSN]-G-H-[PLIVMF]-[GSTA]-x(2)- CONSENSUS [LIMC]-[GS] NAME Transketolase signamre 2 CONSENSUS G-[DEQGSA]-[DN]-G-[PAEQ]-[ST]-[HQ]-x-[PAGM]-[LιVMYAC]-[DEFY ]-x(2)- CONSENSUS [STAP]-x(2)-[RGA] NAME Transaldolase signamre 1 CONSENSUS [DG]-[rVSA]-T-[ST]-N-P-[STA]-[LIVMF](2) NAME Transaldolase active site CONSENSUS [LIVM]-x-[LrVM]-K-[LIVM]-[PAS]-x-[ST]-x-[DENQPAS]-G-[LIVM]-x-[AGV]-x- CONSENSUS [QEKRST]-x-[LIVM] NAME Acyltransferases ChoActase / COT / CPT family signamre 1 CONSENSUS [LI]-P-x-[LVP]-P-[IVTA]-P-x-[LrVM]-x-[DENQAS]-[ST]-[LIVM]-x(2)-[LY] NAME Acyltransferases ChoActase / COT / CPT family signamre 2 CONSENSUS R-[FYW]-x-[DA]-[KA]-x(0,l)-[LrVMFY]-x-[LIVMFY](2)-x(3)-[DNS]-[GSA]-x(6)- CONSENSUS [DE]-[HS]-x(3)-[DE]-[GA] NAME Thiolases acyl-enzyme intermediate signamre CONSENSUS [LIVM]-[NST]-x(2)-C-[SAGLI]-[ST]-[SAG]-[LIVMFYNS]-x-[STAG]-[LIVM]-x(6)- CONSENSUS [LIVM] NAME Thiolases signamre 2 CONSENSUS N-x(2)-G-G-x-[LIVM]-[SA]-x-G-H-P-x-G-x-[ST]-G NAME Thiolases acnve site CONSENSUS [AG]-[LIVMA]-[STAGLIVM]-[STAG]-[LIVMA]-C-x-[AG]-x-[AG]-x-[AG]-x-[SAG] NAME Chloramphenicol acetyltransferase acnve site CONSENSUS Q-[LIV]-H-H-[SA]-x(2)-D-G-[FY]-H NAME Hexapeptide-repeat contaimng-transferases signamre CONSENSUS [LιV]-[GAED]-x(2)-[STAV]-x-[LIV]-x(3)-[LIVAC]-x-[LIV]-[GAED]-x(2)- CONSENSUS [STAVR]-x-[LIV]-[GAED]-x(2)-[STAV]-x-[LIV]-x(3)-[LIV] NAME Beta-ketoacyl synthases active site CONSENSUS G-x(4)-[LIVMFAP]-x(2)-[AGC]-C-[STA](2)-[STAG]-x(3)-[LIVMF] NAME Chalcone and stilbene synthases active site CONSENSUS R-[LIVMFYS]-x-[LIVM]-x-[QHG]-x-G-C-[FYNA]-[GA]-G-[GA]-[STAV]-x-[LIVMF]- CONSENSUS [RA] NAME Myπstoyl-CoA protein N-myπstoyltransferase signamre 1 CONSENSUS E-I-N-F-L-C-x-H-K NAME Myπstoyl-CoA protein N-myπstoyltransferase signamre 2 CONSENSUS K-F-G-x-G-D-G NAME Gamma-glutamyltranspeptidase signamre CONSENSUS T-[STA]-H-x-[ST]-[LIVMA]-x(4)-G-[SN]-x-V-[STA]-x-T-x-T-[LIVM]-[NE]- CONSENSUS x(l,2)-[FY]-G NAME Transglutaminases active site CONSENSUS [GT]-Q-[CA]-W-V-x-[SA]-[GA]-[IVT]-x(2)-T-x-[LMSC]-R-[CSA]-[LV]-G NAME Phosphorylase pyπdoxal-phosphate attachment site CONSENSUS E-A-[SC]-G-x-[GS]-x-M-K-x(2)-[LM]-N NAME UDP-glycosyltransferases signamre CONSENSUS [F ]-x(2)-Q-x(2)-[LIVMYA]-[LIMV]-x(4,6)-[LVGAC]-[LVFYA]-[LIVMF]-[STAGCM]- CONSENSUS [HNQ]-[STAGC]-G-x(2)-[STAG]-x(3)-[STAGL]-[LIVMFA]-x(4)-[PQR]-[LIVMT]- CONSENSUS x(3)-[PA]-x(3)-[DES]-[QEHN] NAME Punne/pyπmidine phosphoπbosyl transferases signamre CONSENSUS [LIVMFY CTA]-[LIVM]-[LIVMA]-[LrVMFC]-[DE]-D-[LIVMS]-[LIVM]-[STAVD]- CONSENSUS [STAR]-[GAC]-x-[STAR] NAME Glutamine amidotransferases class-I active site CONSENSUS [PAS]-[LIVMFYT]-[LIVMFY]-G-[LIVMFY]-C-[LIVMFYN]-G-x-[QEH]-x-[LIVMFA] NAME Glutamine amidotransferases class-II active site CONSENSUS < x(0, 11 )-C-[GS]-[IV]-[LIVMFY ]-[AG] NAME Purme and other phosphorylases family 1 signamre CONSENSUS [GST]-x-G-[LIVM]-G-x-[PA]-S-x-[GSTA]-I-x(3)-E-L NAME Purme and other phosphorylases family 2 signamre CONSENSUS [LIV]-x(3)-G-x(2)-H-x-[LIVMFY]-x(4) [LIVMF]-x(3)-[ATV]-x(l ,2)-[LIVM]-x- CONSENSUS [ATV]-x(4)-[GN]-x(3,4)-[LIVMF](2)-x(2)-[STN]-[SA]-x-G-[GS]-[LIVM] NAME Thymidine and pyπmidine-nucleoside phosphorylases signamre CONSENSUS S-[GS]-R-[GA]-[LIV]-x(2)-[TA]-[GA]-G-T-x-D-x-[LIV]-E NAME ATP phosphoribosyltransferase signature CONSENSUS E-x(5)-G-x-[SAG]-x(2)-[rV]-x-D-[LIV]-x(2)-[ST]-G-x-T-[LM] NAME NAD arginine ADP-nbosyltransferases signamre CONSENSUS [FY]-x-[FY]-K-x(2)-H-[FY]-x-L-[ST]-x-A NAME Prohpoprotein diacylglyceryl transferase signamre CONSENSUS G-R-x-[GA]-N-F-[LIVMF]-N-x-E-x(2)-G NAME S-adenosylmethiomne synthetase signamre 1 CONSENSUS G-A-G-D-Q-G-x(3)-G-Y NAME S-adenosylmethiomne synthetase signamre 2 CONSENSUS G-[GA]-G-[ASC]-F-S-x-K-[DE] NAME Polyprenyl synthetases signamre 1 CONSENSUS [LIVM](2)-x-D-D-x(2,4)-D-x(4)-R-R-[GH] NAME Polyprenyl synthetases signamre 2 CONSENSUS [LIVMFY]-G-x(2)-[FYL]-Q-[LIVM]-x-D-D-[LIVMFY]-x-[DNG] NAME Squalene and phytoene synthases signamre 1 CONSENSUS Y-[CSAιM]-x(2)-[VSG]-A-[GSA]-[LIVAT]-[IV]-G-x(2)-[LMSC]-x(2)-[LIV] NAME Squalene and phytoene synthases signamre 2 CONSENSUS [LIVM]-G-x(3)-Q-x(2,3)-N-[IF]-x-R-D-[LIVMFY]-x(2)-[DE]-x(4,7)-R-x-[FY]- CONSENSUS x-P NAME Protein prenyltransferases alpha subumt repeat signamre CONSENSUS [PSIAV]-X-[NDFV]-[NEQIY]-X-[LΓVMAGP]-W-[NQSTHF]-[FYHQ]-[LIVMR] NAME Riboflavin synthase alpha chain family signamre CONSENSUS [LΓVMF]-X(5)-G-[STADNQ]-[KREQIYW]-V-N-[LIVM]-E NAME Dihydropteroate synthase signamre 1 CONSENSUS [LIVM]-x-[AG]-[LIVMF](2)-N-x-T-x-D-S-F-x-D-x-[SG] NAME Dihydropteroate synthase signamre 2 CONSENSUS [GE]-[SA]-x-[LIVM](2)-D-[LIVM]-G-[GP]-x(2)-[STA]-x-P NAME EPSP synthase signamre 1 CONSENSUS [LIVM]-x(2)-[GN]-N-[SA]-G-T-[STA]-x-R-x-[LIVMY]-x-[GSTA] NAME EPSP synthase signamre 2 CONSENSUS [KR]-x-[KH]-E-[CST]-[DNE]-R-[LIVM]-x-[STA]-[LIVMC]-x(2)-[EN]-[LIVMF]-x- CONSENSUS [KRA]-[LIVMF]-G NAME FLAP/GST2/LTC4S family signamre CONSENSUS G-x(3)-F-E-R-V-[FY]-x-A-[NQ]-x-N-C NAME Aminotransferases class-I pyndoxal-phosphate attachment site CONSENSUS [GS]-[LIVMFYTAC]-[GSTA]-K-x(2)-[GSALVN]-[LIVMFA]-x-[GNAR]-x-R-[LIVMA]- CONSENSUS [GA] NAME Aminotransferases class-II pyndoxal-phosphate attachment site CONSENSUS T-[LIVMFY ]-[STAG]-K-[SAG]-[LIVMFYWR]-[SAG]-x(2)-[SAG] NAME Aminotransferases class-Ill pyndoxal-phosphate attachment site CONSENSUS [LIVMFYWC](2)-x-D-E-[LIVMA] x(2)-[GP]-x(0,l)-[LIVMFYWAG]-x(0,l)-[SACR]-x- CONSENSUS [GSAD]-x(12,16)-D-[LIVMFYWC]-x(2,3)-[GSA]-K-x(3)-[GSTADN]-[GSA] NAME Aminotransferases class-IV signamre CONSENSUS E-x-[STAGCI]-x(2)-N-[LIVMFAC]-[FY]-x(6,12)-[LIVMF]-x-T-x(6,8)-[LIVM]-x- CONSENSUS [GS]-[LIVM]-x-[KR] NAME Aminotransferases class-V pyndoxal-phosphate attachment site CONSENSUS [LIVFYCHT]-[DGH]-[LIVMFYAC]-[LIVMFYA]-x(2)-[GSTAC]-[GSTA]-rHQR]-K- CONSENSUS x(4,6)-G-x-[GSAT]-x-[LIVMFYSAC] NAME Hexokinases signamre CONSENSUS [LIVMJ-G-F [TN]-F-S-[FY]-P-x(5)-[LIVM]-[DNST]-x(3)-[LIVM]-x(2)-W-T-K-x- CONSENSUS [LF] NAME Galactokinase signamre CONSENSUS G-R-x-N-[LIV]-I-G-E-H-x-D-Y NAME GHMP kinases putative ATP-bindmg domain CONSENSUS [LIVM]-[PK]-X-[GSTA]-X(0, 1)-G-L-[GS]-S-S-[GSA]-[GSTAC] NAME Phosphofructokinase signature CONSENSUS [RK]-x(4)-G-H-x-Q-[QR]-G-G-x(5)-D-R NAME pfkB family of carbohydrate kinases signamre 1 CONSENSUS [AG]-G-x(0, l)-[GAP]-x-N-x-[STA]-x(6)-[GS]-x(9)-G NAME pfkB family of carbohydrate kinases signamre 2 CONSENSUS [DNSK]-[PSTV]-x-[SAG](2)-[GD]-D-x(3)-[SAGV]-[AG]-[LIVMFY]-[LIVMSTAP] NAME ROK family signamre CONSENSUS [LIVM]-x(2)-G-[LIVMFCT]-G-x-[GA]-[LIVMFA]-x(8)-G-x(3,5)-[GATP]-x(2)- CONSENSUS G-IRKH] NAME Phosphoπbulokinase signamre CONSENSUS K-[LIVM]-x-R-D-x(3)-R-G-x-[ST]-x-E NAME Thymidine kmase cellular-type signamre CONSENSUS [GA]-x(l,2)-[DE]-x-Y-x-[STAP]-x-C-[NKR]-x-[CH]-[LIVMFYWH] NAME FGGY family of carbohydrate kinases signamre 1 CONSENSUS rMFYGS]-x-[PST]-x(2)-K-[LIVMFYW]-x- -[LIVMF]-x-[DENQTKR]-[ENQH] NAME FGGY family of carbohydrate kinases signamre 2 CONSENSUS [GSA]-x-[LIVMFYW]-x-G-[LIVM]-x(7,8)-[HDENQ]-[LIVMF]-x(2)-[AS]-[STAIVM]- CONSENSUS [LIVMFY]-[DEQ] NAME- Protein kinases ATP-binding region signamre CONSENSUS. [LΓV]-G-{P}-G-{P}-[FYWMGSTNH]-[SGA]-{PW}-[LIVCAT]-{PD}-X-[GSTACLΓVMFY]- CONSENSUS- x(5, 18)-[LIVMFYWCSTAR]-[AΓVP]-[LIVMFAGCKR]-K. NAME: Seπne/Threonine protein kinases active-site signamre CONSENSUS: [LrVMFYC]-x-[HY]-x-D-[LIVMFY]-K-x(2)-N-[LIVMFYCT](3) NAME: Tyrosine protein kinases specific active-site signamre CONSENSUS [LIVMFYC]-x-[HY]-x-D-[LIVMFY]-[RSTAC]-x(2)-N-[LIVMFYC](3). NAME Protein kinase domain profile NAME' Casein kinase II regulatory subunit signamre. CONSENSUS: C-P-x-[LIVMY]-x-C-x(5)-L-P-[LIVMC]-G-x(9)-V-[KR]-x(2)-C-P-x-C. NAME: Pyruvate kinase active site signamre CONSENSUS: [LIVAC]-x-[LIVM](2)-[SAPCV]-K-[LIV]-E-[NKRST]-x-[DEQH]-[GSTA]-[LIVM] NAME Shikimate kinase signamre CONSENSUS: [KR]-x(2)-E-x(3)-[LIVMF]-x(8,12)-[LIVMF](2)-[SA]-x-G(3)-x-[LIVMF] NAME Prokaryotic diacylglycerol kinase signamre CONSENSUS E-x-[LIVM]-N-[ST]-[SA]-[LIV]-E-x(2)-V-D. NAME Phosphatidylinositol 3- and 4-kmases signamre 1 CONSENSUS [LIVMFAC]-K-x(l ,3)-[DEA]-[DE]-[LIVMC]-R-Q-[DE]-x(4)-Q. NAME- Phosphatidylinositol 3- and 4-kmases signamre 2. CONSENSUS [GS]-x-[AV]-x(3)-[LIVM]-x(2)-[FYH]-[LIVM](2)-x-[LIVMF]-x-D-R-H-x(2)-N NAME: Acetate and butyrate kinases family signamre 1 CONSENSUS [LIVM](2)-x-[LIVM]-N-x-G-S-[ST]-S-x-[KE]. NAME: Acetate and butyrate kinases family signamre 2 CONSENSUS: [LIVMA](2)-x(2)-H-x-G-x-G-x-[ST]-[LIVM]-x-[AV]-x(3)-G Phosphoglycerate kinase signamre CONSENSUS: [KRHGTCV]-[VT]-[LIVMF]-[LIVMC]-R-x-D-x-N-[SACV]-P. NAME: Aspartokinase signamre CONSENSUS- [LIVM]-x-K-[FY]-G-G-[ST]-[SC]-[LIVM] NAME Glutamate 5-kιnase signamre CONSENSUS: [GSTN]-x(2)-G-x-G-[GC]-[IM]-x-[STA]-K-[LIVM]-x-[SA]-[TCA]-x(2)-[GALV]- CONSENSUS: x(3)-G NAME ATP.guanido phosphotransferases active site CONSENSUS: C-P-x(0, 1)-[ST]-N-[IL]-G-T. NAME PTS HPR component histidine phosphorylation site signamre CONSENSUS: G-[LIVM]-H-[STA]-R-[PA]-[GSTA]-[STAM] . NAME PTS HPR component serine phosphorylation site signamre CONSENSUS [GSADE]-[KREQTV]-x(4)-[KRN]-S-[LIVMF](2)-x-[LIVM]-x(2)-[LIVM]-[GAD] NAME PTS EIIA domains phosphorylation site signamre 1 CONSENSUS: G-x(2)-[LIVMF](3)-H-[LIVMF]-G-[LrVMF]-x-T-[ALV] . NAME PTS EIIA domains phosphorylation site signamre 2 CONSENSUS: [DENQ]-x(6)-[LIVMF]-[GA]-x(2)-[LIVM]-A-[LIVM]-P-H-[GAC]. NAME: PTS EIIB domains cysteine phosphorylation site signamre CONSENSUS: N-[LIVMFY]-x(5)-C-x-T-R-[LIVMF]-x-[LIVMF]-x-[LIVM]-x-[DQ] NAME: Adenylate kinase signamre. CONSENSUS: [LrVMFYW](3)-D-G-[FYI]-P-R-x(3)-[NOJ . NAME: Nucleoside diphosphate kinases active site CONSENSUS. N-x(2)-H-[GA]-S-D-[SA]-[LIVMPKNE] . NAME: Guanylate kinase signamre. CONSENSUS: T-[ST]-R-x(2)-[KR]-x(2)-[DE]-x(2)-G-x(2)-Y-x-[FY]-[LIVMK]. NAME Guanylate kinase domam profile NAME Phosphoπbosyl pyrophosphate synthetase signamre CONSENSUS D-[LI]-H-[SA]-x-Q-[IMST]-[QM]-G-[FY]-F-x(2)-P-[LIVMFC]-D NAME 7,8-dιhydro-6-hydroxymethylpteπn-pyrophosphokιnase signamre CONSENSUS G-[PE]-R-x(2)-D-L-D-[LIVM](2) NAME Bacteπophage-type RNA polymerase family active site signamre 1 CONSENSUS P-[LIVM]-x(2)-D-[GA]-[ST]-[AC]-[SN]-[GA]-[LIVMFY]-Q NAME Bacteπophage-type RNA polymerase family active site signamre 2 CONSENSUS [LIVMF]-x-R-x(3)-K-x(2)-[LIVMF]-M-[PT]-x(2)-Y NAME Eukaryotic RNA polymerase II heptapeptide repeat CONSENSUS Y-[ST]-P-[ST]-S-P-[STANK] NAME RNA polymerases beta chain signamre CONSENSUS G-x-K-[LιVMFA]-[STAC]-[GSTN]-x-[HSTA]-[GS]-[QNH]-K-G-[IVT] NAME RNA polymerases M / 15 Kd subunits signamre CONSENSUS F-C-x-[DEKST]-C-[GNK]-[DNSA}-[LIVMH]-[LIVM]-x(8,14)-C-x(2)-C NAME RNA polymerases D / 30 to 40 Kd subunits signamre CONSENSUS N-[SGA]-[LIVMF]-R-R-x(9)-[SA]-x(3)-V-x(4)-N-x-[STA]-x(3)-[DN]-E-x-[Ln- CONSENSUS [GA]-x-R-[LI]-[GA]-[LIVM](2)-P NAME RNA polymerases H / 23 Kd subunits signamre CONSENSUS H-[NEI]-[LIVM]-V-P-x-H-x(2)-[LIVM]-x(2)-[DE] NAME RNA polymerases K / 14 to 18 Kd subunits signamre CONSENSUS [ST]-x-[FY]-E-x-[AT]-R-x-[LIVM]-[GSA]-x-R-[SA]-x-Q NAME RNA polymerases L / 13 to 16 Kd subunits signamre CONSENSUS [DE](2)-H-[ST]-[LIVM]-[GAP]-N-x(l 1) V-x-[FM]-x(2)-Y-x(3)-H-P NAME RNA polymerases N / 8 Kd subumts signamre CONSENSUS [LIVMF](2)-P-[LIVM]-x-C-F-[ST]-C-G NAME DNA polymerase family A signamre CONSENSUS R-x(2)-[GSAV]-K-x(3)-[LIVMFY]-[AGQ] x(2)-Y-x(2)-[GS]-x(3)-[LIVMA] NAME DNA polymerase family B signamre CONSENSUS [YA]-[GLIVMSTAC]-D-T-D-[SG]-[LIVMFTC]-x-[LIVMSTAC] NAME DNA polymerase family X signamre CONSENSUS G-[SG]-[LFY]-x-R-[GE]-x(3)-[SGCL]-x-D-[LIVM]-D-[LIVMFY](3)-x(2)-[SAP] NAME Galactose- 1-phosphate uridyl transferase family 1 active site signamre CONSENSUS F-E-N-[RK]-G-x(3)-G-x(4)-H-P-H-x-Q NAME Galactose- 1-phosphate uridyl transferase family 2 signature CONSENSUS D-L-P-I-V-G-G-[ST]-[LIVM](2)-[SA]-H-[DEN]-H-[FY]-Q-G-G NAME ADP-glucose pyrophosphorylase signamre 1 CONSENSUS [AG]-G-G-x-G-[STK]-x-L-x(2)-L-[TA]-x(3)-A-x-P-A-[LV] NAME ADP-glucose pyrophosphorylase signamre 2 CONSENSUS W-[FY]-x-G-[ST]-A-[DNSH]-[AS]-[LIVMFYW] NAME ADP-glucose pyrophosphorylase signamre 3 CONSENSUS [APV]-[GS]-M-G-[LIVMN]-Y-[rVC]-[LIVMFY]-x(2)-[DENPHK] NAME Phosphatidate cytidylyltransferase signamre CONSENSUS S-x-[LIVMF]-K-R-x(4)-K-D-x-[GSA]-x(2)-[LI]-[PG]-x-H-G-G-[LIVM]-x-D-R- CONSENSUS [LIVMFT]-D NAME Ribonuclease PH signature CONSENSUS C-[DE]-[LIVM](2)-Q-[GTA]-D-G-[SG]-x(2)-[TA]-A NAME 2'-5'-olιgoadenylate synthetases signamre 1 CONSENSUS G-G-S-x-[AG]-[KR]-x-T-x-L-[KR]-[GST]-x-S-D-[AG] NAME 2'-5'-olιgoadenylate synthetases signamre 2 CONSENSUS R-P-V-I-L-D-P-x-[DE]-P-T NAME CDP-alcohol phosphatidyltransferases signamre CONSENSUS D-G-x(2)-A-R-x(8)-G-x(3)-D-x(3)-D NAME PEP-utilizmg enzymes phosphorylation site signamre CONSENSUS G-[GA]-x-[TN]-x-H-[STA]-[STAV]-[LIVM](2)-[STAV]-[RG] NAME PEP-uti zing enzymes signamre 2 CONSENSUS [DEQS]-x-[LιVMF]-S-[LIVMF]-G-[ST]-N-D-[LIVM]-x-Q-[LrVMFYGT]-[STALIV]- CONSENSUS [LIVMF]-[GAS]-x(2)-R NAME Rhodanese signamre 1 CONSENSUS [FY]-x(3)-H-[LIV]-P-G-A-x(2)-[LIVF] NAME Rhodanese C-terminal signamre CONSENSUS [AV]-x(2)-[FY]-[DEAP]-G-[GSA]-[WF]-x-E-[FYW] NAME CoA transferases signature 1 CONSENSUS [DN]-[GN]-x(2)-[LIVMFA](3)-G-G-F-x(3)-G-x-P NAME CoA transferases signature 2 CONSENSUS [LF]-[HQ]-S-E-N-G-[LIVF](2)-[GA] NAME Phospholipase A2 histidine active site CONSENSUS C-C-x(2)-H-x(2)-C NAME Phospholipase A2 aspartic acid active site CONSENSUS [LIVMA]-C-{LrVMFYWPCST}-C-D-x(5)-C NAME Lipases, serine active site CONSENSUS [LIV]-x-[LIVFY]-[LIVMST]-G-[HYWV]-S-x-G-[GSTAC] NAME Co pase signamre CONSENSUS Y-x(2)-Y-Y-x-C-x-C NAME Lipolytic enzymes "G-D-S-L" family, serme active site CONSENSUS [LIVMFYAG](4)-G-D-S-[LIVM]-x(l ,2)-[TAG]-G NAME Lipolytic enzymes "G-D-X-G" family, putative histidine active site CONSENSUS [LIVMF](2)-x-[LIVMF]-H-G-G-[SAG]-[FY]-x(3)-[STDN]-x(2)-[ST]-H NAME Lipolytic enzymes "G-D-X-G" family, putative serine active site CONSENSUS [LrVM]-x-[LIVMF]-[SA]-G-D-S-[CA]-G-[GA]-x-L-[CA] NAME Carboxylesterases type-B serine active site CONSENSUS F-[GR]-G-x(4)-[LIVM]-x-[LIV]-x-G-x-S-[STAG]-G NAME Carboxylesterases type-B signamre 2 CONSENSUS [ED]-D-C-L-[YT]-[LIV]-[DNS]-[LIV]-[LIVFYW]-x-[PQR] NAME Pectinesterase signamre 1 CONSENSUS [GSTN]-x(5)-[LIVM]-x-[LIVM]-x(2)-G-x-Y-[DNK]-E-x-[LrVM]-x-[LIVM] NAME Pectinesterase signamre 2 CONSENSUS G-[STAD]-[LIVMT]-D-F-I-F-G NAME Peptidyl-tRNA hydrolase signamre 1 CONSENSUS [FY]-x(2)-T-R-H-N-x-G-x(2)-[LIVMFA](2)-[DE] NAME Peptidyl-tRNA hydrolase signamre 2 CONSENSUS [GS]-x(3)-H-N-G-[LIVM]-[KR]-[DNS}-[LIVMT] NAME Alkaline phosphatase active site CONSENSUS [IV]-x-D-S-[GAS]-[GASC]-[GAST]-[GA]-T NAME Histidine acid phosphatases phosphohistidme signamre CONSENSUS [LIVM]-x(2)-[LIVMA]-x(2)-[LIVM]-x-R-H-[GN]-x-R-x-[PAS] NAME Histidine acid phosphatases active site signamre CONSENSUS [LIVMF]-x-[LIVMFAG]-x(2)-[STAGI]-H-D-[STANQ]-x-[LIVM]-x(2)-[LIVMFY]-x(2)- CONSENSUS [STA] NAME Class A bacterial acid phosphatases signamre CONSENSUS G-S-Y-P-S-G-H-T NAME 5'-nucleotιdase signamre 1 CONSENSUS [LIVM]-x-[LIVM](2)-[HEA]-[TI]-x-D-x-H-[GSA]-x-[LrVMF] NAME 5'-nucleotιdase signamre 2 CONSENSUS [FYP]-x(4)-[LIVM]-G-N-H-E-F-[DN] NAME Fructose- 1-6-bιsphosphaιase active site CONSENSUS [AG]-[RK]-L-x(l,2)-[LIV]-[FY]-E-x(2)-P-[LIVM]-[GSA] NAME Serine/threomne specific protein phosphatases signamre CONSENSUS [LIVM]-R-G-N-H-E NAME Protein phosphatase 2A regulatory subumt PR55 signamre 1 CONSENSUS E-F-D-Y-L-K-S-L-E-I-E-E-K-I-N NAME Protein phosphatase 2A regulatory subumt PR55 signamre 2 CONSENSUS N-[AG]-H-[TA]-Y-H-I-N-S-I-S-[LIVM]-N-S-D NAME Protem phosphatase 2C signamre CONSENSUS [LIVMFY]-[LIVMFYA]-[GSAC]-[LIVM]-[FYC]-D-G-H-[GAV] NAME Tyrosine specific protein phosphatases active site CONSENSUS [LIVMF]-H-C-x(2)-G-x(3)-[STC]-[STAGP]-x-[LIVMFY] NAME Tyrosine specific protein phosphatases profile NAME Dual specificity protein phosphatase profile NAME PTP type protein phosphatase profile NAME Inositol monophosphatase family signamre 1 CONSENSUS [FWV]-x(0,l)-[LIVM]-D-P-[LIVM]-D-[SG]-[ST]-x(2)-[FY]-x-[HKRNSTY] NAME Inositol monophosphatase family signamre 2 CONSENSUS [WV]-D-x-[AC]-[GSA]-[GSAPV]-x-[LIVACP]-[LIV]-[LIVAC]-x(3)-[GH]-[GA] NAME Prokaryotic zinc-dependent phospholipase C signamre CONSENSUS H-Y-x-[GT]-D-[LIVM]-[DNS]-x-P-x-H-[PA]-x-N NAME Phosphatidylinositol-specific phospholipase X-box domain profile NAME Phosphatidylinositol-specific phospholipase Y-box domain profile NAME 3'5'-cychc nucleotide phosphodiesterases signamre CONSENSUS H-D-[LIVMFY]-x-H-x-[AG]-x(2)-[NQ]-x-[LIVMFY] NAME cAMP phosphodiesterases class-II signamre CONSENSUS H-x-H-L-D-H-[LIVM]-x-[GS]-[LIVMA]-[LIVM](2)-x-S-[AP] NAME Sulfatases signature 1 CONSENSUS [SAP]-[LIVMST]-[CS]-[STAC]-P-[STA]-R-x(2)-[LIVMFW](2)-[TR]-G NAME Sulfatases signature 2 CONSENSUS G-[YV]-x-[ST]-x(2)-[IVA]-G-K-x(0, 1)-[FYWK]-[HL] NAME AP endonucleases family 1 signamre 1 CONSENSUS [APF]-D-[LIVMF](2)-x-[LIVM]-Q-E-x-K NAME AP endonucleases family 1 signamre 2 CONSENSUS D-[ST]-[FY]-R-[KH]-x(7,8)-[FYW]-[ST]-[FYW](2) NAME AP endonucleases family 1 signamre 3 CONSENSUS N-x-G-x-R-[LrVM]-D-[LIVMFYH]-x-[LV]-x-S NAME AP endonucleases family 2 signature 1 CONSENSUS H-x(2)-Y-[LIVMF]-[IM]-N-[LIVMCA]-[AG] NAME AP endonucleases family 2 signamre 2 CONSENSUS [GR]-[LIVMF]-C-[LIVM]-D-T-C-H NAME AP endonucleases family 2 signamre 3 CONSENSUS [LIVMW]-H-x-N-[DE]-[SA]-K-x(3)-G-[SA]-x(2)-D NAME Deoxyπbonuclease I signamre 1 CONSENSUS [LIVM](2)-[AP]-L-H-[STA](2)-P-x(5)-E-[LIVM]-[DN]-x-L-x-[DE]-V NAME Deoxynbonuclease I signamre 2 CONSENSUS G-D-F-N-A-x-C-[SA] NAME Endonuclease III iron-sulfur binding region signamre CONSENSUS C-x(3)-[KRS]-P-[KRAGL]-C-x(2)-C-x(5)-C NAME Endonuclease III family signamre CONSENSUS [GST]-x-[LιVMF]-P-x(5)-[LIVMW]-x(2,3)-[Lη-[PAS]-G-V-[GA]-x(3)-[GAC]- CONSENSUS x(3)-[LIVM]-x(2)-[SALV]-[LIVMFYW]-[GANK] NAME Ribonuclease II family signamre CONSENSUS [HI]-[FYE]-[GSTAM]-[LIVM]-x(4,5)-Y-[STAL]-x-[FWVAC]-[TV]-[SA]-P-[LIVMA]- CONSENSUS [RQ]-[KR]-[FY]-x-D-x(3)-[HQ] NAME Ribonuclease III family signamre CONSENSUS [DEQ]-[RQ]-[LM]-E-[FYW]-[LV]-G-D-[SAR] NAME Bacterial Ribonuclease P protein component signamre CONSENSUS [LIVMFYS]-x(2)-A-x(2)-R-[NH]-[KRQL]-[LIVM]-[KRA]-R-x-[LIVMTA]-[KR] NAME Ribonuclease T2 family histidine active site 1 CONSENSUS [FYWL]-X-[LΓVM]-H-G-L-W-P NAME Ribonuclease T2 family histidine active site 2 CONSENSUS [LIVMF]-x(2)-[HDGTY]-[EQ]-[FYW]-x-[KR]-H-G-x-C NAME Pancreatic πbonuclease family signamre CONSENSUS C-K-x(2)-N-T-F NAME DNA/RNA non-specific endonucleases active site CONSENSUS D-R-G-H-[QIL]-x(3)-A NAME Thermonuclease family signamre 1 CONSENSUS D-G-D-T-[LIVM]-x-[LIVMC]-x(9,10)-R-[LIVM]-x(2)-[LIVM]-D-x-P-E NAME Thermonuclease family signamre 2 CONSENSUS D [KR]-Y-[GQ]-R-x-[LV]-[GA]-x-[IV]-[FYW] NAME Beta-amylase active site 1 CONSENSUS H-x-C-G-G-N-V-G-D NAME Beta-amylase active site 2 CONSENSUS G-x-[SA]-G-E-[LIVM]-R-Y-P-S-Y NAME Glucoa ylase active site region signature CONSENSUS [STN]-[GP]-x(l ,2)-[DE]-x-W-E-E-x(2)-[GS] NAME Polygalacturonase active site CONSENSUS [GSDENKRH]-x(2)-[VMFC]-x(2)-[GS]-H-G-[LIVMAG]-x(l,2)-[LIVM]-G-S NAME Clostridium cellulosome enzymes repeated domain signamre CONSENSUS D-[LIVMFY]-[DNV]-x-[DNS]-x(2)-[LIVM]-[DN]-[SALM]-x-D-x(3)-[LIVMF]-x- CONSENSUS [RKS]-x-[LIVMF] NAME Chitinases family 18 active site CONSENSUS [LIVMFY]-[DN]-G [LIVMF]-[DN]-[LIVMF]-[DN]-x-E NAME Chinnases family 19 signamre 1 CONSENSUS C-x(4,5)-F-Y-[ST]-x(3)-[FY]-[LIVMF]-x-A-x(3)-[YF]-x(2)-F-[GSA] NAME Chitinases family 19 signamre 2 CONSENSUS [LΓVM]-[GSA]-F-X-[STAG](2)-[LIVMFY]-W-[FY]-W-[LIVM] NAME Alpha-lactalbumm / lysozyme C signamre CONSENSUS C-x(3)-C-x(2)-[LMF]-x(3)-[DEN]-[LI]-x(5)-C NAME Alpha-galactosidase signamre CONSENSUS G-[LrVMFY]-x(2)-[LIVMFY]-x-[LIVM]-D-D-x-W-x(3,4)-R-[DNSF] NAME Trehalase signamre 1 CONSENSUS: P-G-G-R-F-x-E-x-Y-x-W-D-x-Y NAME Trehalase signamre 2. CONSENSUS Q-W-D-x-P-x-[GA]-W-[PA]-P. NAME Alpha-L-fucosidase putative active site. CONSENSUS : P-x(2)-L-x(3)-K-W-E-x-C . NAME' Glycosyl hydrolases family 1 active site CONSENSUS [LIVMFSTC]-[LIVFYS]-[LIV]-[LIVMST]-E-N-G-[LIVMFAR]-[CSAGN]. NAME: Glycosyl hydrolases family 1 N-terminal signamre CONSENSUS: F-x-[FYWM]-[GSTA]-x-[GSTA]-x-[GSTA](2)-[FYNH]-[NQ]-x-E-x-[GSTA]. NAME Glycosyl hydrolases family 2 signamre 1 CONSENSUS. N-x-[LIVMFYWD]-R-[STACN](2)-H-Y-P-x(4)-[LIVMFYW](2)-x(3)-[DN]-x(2)- CONSENSUS- G-[LIVMFYW](4). NAME. Glycosyl hydrolases family 2 acid/base catalyst. CONSENSUS [DENQF]-[KRVW]-N-H-[AP]-[SAC]-[LIVMF](3)-W-[GS]-x(2,3)-N-E NAME- Glycosyl hydrolases family 3 active site. CONSENSUS [LIVM](2)-[KR]-x-[EQK]-x(4)-G-[LIVMFT]-[LιVT]-[LIVMF]-[ST]-D-x(2)- CONSENSUS [SGADNI]. NAME: Glycosyl hydrolases family 5 signamre CONSENSUS [LIV]-[LIVMFYWGA](2)-[DNEQG]-[LIVMGST]-x-N-E-[PV]-[RHDNSTLIVFY]. NAME: Glycosyl hydrolases family 6 signamre 1 CONSENSUS. V-x-Y-x(2)-P-x-R-D-C-[GSAF]-x(2)-[GSA](2)-x-G. NAME: Glycosyl hydrolases family 6 signamre 2 CONSENSUS. [LIVMYA]-[LIVA]-[LIVT]-[LIV]-E-P-D-[SAL]-[LI]-[PSAG] NAME- Glycosyl hydrolases family 8 signamre. CONSENSUS A-[ST]-D-[AG]-D-x(2)-[IM]-A-x-[SA]-[LIVM]-[LIVMG]-x-A-x(3)-[FW] NAME Glycosyl hydrolases family 9 active sites signamre 1 CONSENSUS [STV]-x-[LIVMFY]-[STV]-x(2)-G-x-[NKR]-x(4)-[PLIVM]-H-x-R. NAME Glycosyl hydrolases family 9 active sites signamre 2. CONSENSUS [FYW]-x-D-x(4)-[FYW]-x(3)-E-x-[STA]-x(3)-N-[STA] NAME Glycosyl hydrolases family 10 active site. CONSENSUS: [GTA]-x(2)-[LIVN]-x-[IVMF]-[ST]-E-[LIY]-[DN]-[LIVMF]. NAME: Glycosyl hydrolases family 11 active site signamre 1. CONSENSUS: [PSA]-[LQ]-x-E-Y-Y-[LIVM](2)-[DE]-x-[FYWHN] NAME: Glycosyl hydrolases family 11 active site signamre 2 CONSENSUS- [LrVMF]-x(2)-E-[AG]-[YWG]-[QRFGS]-[SG]-[STAN]-G-x-[SAF]. NAME: Glycosyl hydrolases family 16 active sites CONSENSUS E-[LIV]-D-[LIV]-x(0,l)-E-x(2)-[GQ]-[KRNF]-x-[PSTA]. NAME: Glycosyl hydrolases family 17 signamre CONSENSUS [LIVM]-x-[LIVMFYWA](3)-[STAG]-E-[STA]-G-W-P-[STN]-x-[SAGQ]. NAME- Glycosyl hydrolases family 25 active sites signamre. CONSENSUS D-[LIVM]-x(3)-[NQ]-[PG]-x(9,10)-G-x(4)-[LIVMFY](2)-K-x-[ST]-E-[GS]-x(2)- CONSENSUS: Y-x-[DN]. NAME Glycosyl hydrolases family 31 active site. CONSENSUS- [GF]-[LIVMF]-W-x-D-M-[NSA]-E. NAME: Glycosyl hydrolases family 31 signamre 2. CONSENSUS: G-[AV]-D-[LIVMT]-C-G-[FY]-x(3)-[ST]-x(3)-L-C-x-R-W-x(2)-[LV]-[GS]-[SA]- CONSENSUS. F-x-P-F-x-R-[DN]. NAME: Glycosyl hydrolases family 32 active site. CONSENSUS: H-x(2)-P-x(4)-[LIVM]-N-D-P-N-G NAME: Glycosyl hydrolases family 35 putative active site. CONSENSUS: G-G-P-[LIVM](2)-x(2)-Q-x-E-N-E-[FY]. NAME Glycosyl hydrolases family 39 active site CONSENSUS W-x-F-E-x-W-N-E-P-[DN] NAME Glycosyl hydrolases family 45 active site CONSENSUS [STA]-T-R-Y-[FYW]-D-x(5)-[CA] NAME Prokaryotic transglycosylases signamre CONSENSUS [LIVM]-x(3)-E-S-x(3)-[AP]-x(3)-S-x(5)-G-[LIVM]-[LIVMFYW]-x-[LIVMFY ]- CONSENSUS x(4)-[SAG] NAME Inosine-uπdine preferring nucleoside hydrolase family signamre CONSENSUS D-x-D-[PT]-[GA]-x-D-D-[TAV]-[VI]-A NAME Alkylbase DNA glycosidases alkA family signamre CONSENSUS G-I-G-x-W-[ST]-[AV]-x-[LIVMFY](2)-x-[LIVM]-x(8)-[MF]-x(2)-[ED]-D NAME Foπnamidopyπmidine-DNA glycosylase signamre CONSENSUS C-x(2,4)-C-x-[GTAQ]-x-[IV]-x(7)-R-[GSTAN]-[STA]-x-[FYI]-C-x(2)-C-Q NAME Uracil-DNA glycosylase signamre CONSENSUS [KR]-[LIV]-[LIVC]-[LIVM]-x-G-[QI]-D-P-Y NAME S-adenosyl-L-homocysteine hydrolase signamre 1 CONSENSUS [CS]-N-x-[FYL]-S-[ST]-[QA]-[DEN]-x-[AV](2)-A-A-[LIV]-[SAV] NAME S-adenosyl-L-homocysteine hydrolase signamre 2 CONSENSUS G-K-x(3)-[LIV]-x-G-Y-G-x-V-G-[KR]-G-x-A NAME Cytosol aminopeptidase signamre CONSENSUS N-T-D-A-E-G-R-L NAME Aminopeptidase P and proline dipeptidase signamre CONSENSUS [HA]-[GSYR]-[LIVMT]-[SG]-H-x-[LIV]-G-[LIVM]-x-[IV]-H-[DE] NAME Methiomne aminopepndase subfamily 1 signature CONSENSUS [MFY]-x-G-H-G-[LIVMC]-[GSH]-x(3)-H-x(4)-[LIVM]-x-[HN]-[YWV] NAME Methiomne aminopeptidase subfamily 2 signature CONSENSUS [DA]-[LrVMY]-x-K-rLIVM]-D-x-G-x-[HQ]-[LIVM]-[DNS]-G-x(3)-[DN] NAME Renal dipeptidase active site CONSENSUS [LIVM]-E-G-[GA]-x(2)-[LIVMF]-x(6)-L-x(3)-Y-x(2)-G-[LIVM]-R NAME Senne carboxypeptidases, serme active site CONSENSUS [LIVM]-x-[GTA]-E-S-Y-[AG]-[GS] NAME Serine carboxypeptidases, histidine active site CONSENSUS [LIVF]-x(2)-[LIVSTA]-x-[IVPST]-x-[GSDNQL]-[SAGV]-[SG]-H-x-[IVAQ]-P-x(3)- CONSENSUS [PSA] NAME Zinc carboxypeptidases, zinc-binding region 1 signamre CONSENSUS [PK]-X-[LΓVMFY]-X-[LIVMFY]-X(4)-H-[STAG]-X-E-X-[LΓVM]-[STAG]-X(6)- CONSENSUS [LIVMFYTA] NAME Zinc carboxypeptidases, zinc-binding region 2 signamre CONSENSUS H-[STAG]-x(3)-[LIVME]-x(2)-[LIVMFYW]-P-[FYW] NAME Senne proteases, trypsin family, histidine active site CONSENSUS [LIVM]-[ST]-A-[STAG]-H-C NAME Serine proteases, trypsin family, senne active site CONSENSUS [DNSTAGC]-[GSTAPIMVQH]-x(2)-G-[DE]-S-G-[GS]-[SAPHV]-[LIVMFYWH]- CONSENSUS [LIVMFYSTANQH] NAME Serme proteases, subnlase family, aspartic acid active site CONSENSUS [STAIV]-x-[LIVMF]-[LrVM]-D-[DSTA]-G-[LIVMFC]-x(2,3)-[DNH] NAME Serine proteases, subtilase family, histidine acnve site CONSENSUS H-G-[STM]-x-rVIC]-[STAGC]-[GS]-x-[LIVMA]-[STAGCLV]-[SAGM] NAME Serine proteases, subnlase family, serme active site CONSENSUS G-T-S-x-[SA]-x-P-x(2)-[STAVC]-[AG] NAME: Serine proteases, V8 family, histidine active site. CONSENSUS [ST]-G-[LIVMFYW](3)-[GN]-x(2)-T-[LIVM]-x-T-x(2)-H. NAME Serine proteases, V8 family, serine active site CONSENSUS. T-X(2)-[GC]-[NQ]-S-G-S-X-[LΓVM]-[FY] NAME: Serine proteases, omptin family signamre 1. CONSENSUS W-T-D-x-S-x-H-P-x-T NAME: Serine proteases, omptin family signamre 2. CONSENSUS- A-G-Y-Q-E-[ST]-R-[FYW]-S-[FYW]-[TN]-A-x-G-G-[ST]-Y NAME: Prolyl endopepndase family serine active site. CONSENSUS. D-x(3)-A-x(3)-[LIVMFYW]-x(14)-G-x-S-x-G-G-[LIVMFYW](2). NAME: Endopeptidase Clp senne active site. CONSENSUS T-x(2)-[LIVMF]-G-x-A-[SAC]-S-[MSA]-[PAG]-[STA] NAME: Endopeptidase Clp histidine acdve site. CONSENSUS R-x(3)-[EAP]-x(3)-[LIVMFYT]-M-[LIVM]-H-Q-P. NAME ATP-dependent serine proteases, Ion family, serine active site. CONSENSUS. D-G-[PD]-S-A-[GS]-[LIVMCA]-[TA]-[LIVM] NAME: Eukaryotic thiol (cysteine) proteases cysteine active site CONSENSUS Q-x(3)-[GE]-x-C-[YW]-x(2)-[STAGC]-[STAGCV] . NAME: Eukaryotic thiol (cysteine) proteases histidine active site. CONSENSUS: [LIVMGSTAN]-x-H-[GSACE]-[LIVM]-x-[LIVMAT](2)-G-x-[GSADNH]. NAME. Eukaryotic thiol (cysteine) proteases asparagine active site CONSENSUS- [FYCH]-ι^q-[LIVT]-x-[KRQAG]-N-[ST]-W-x(3)-[FY ]-G-x(2)-G-[LFYW]- CONSENSUS: [LIVMFYG]-x-[LIVMF]. NAME. Ubiquitin carboxyl-terminal hydrolase family 1 cysteine active-site CONSENSUS: Q-x(3)-N-[SA]-C-G-x(3)-[LIVM](2)-H-[SA]-[LIVM]-[SA] . NAME Ubiquitin carboxyl-terminal hydrolases family 2 signamre 1 CONSENSUS G-[LΓVMFY]-X(1,3)-[AGC]-[NASM]-X-C-[FYW]-[LIVMC]-[NST]-[SACV]-X-[LIVMS]- CONSENSUS Q NAME Ubiquitin carboxyl-terminal hydrolases family 2 signamre 2. CONSENSUS Y-X-L-X-[SAG]-[LIVMFT]-X(2)-H-X-G-X(4,5)-G-H-Y. NAME Caspase family histidine active site CONSENSUS H-x(2,4)-[SC]-x(4)-[LIVMF](2)-[ST]-H-G. NAME: Caspase family cysteine active site. CONSENSUS. K-P-K-[LIVMF](4)-Q-A-C-[RQG]-G NAME. Eukaryotic and viral aspartyl proteases active site CONSENSUS [LIVMFGAC]-[LIVMTADN]-[LIVFSA]-D-[ST]-G-[STAV]-[STAPDENQ]-x-[LIVMFSTNC]- CONSENSUS. x-[LIVMFGTA]. NAME: Neutral zinc metallopeptidases, zinc-bmding region signamre CONSENSUS: [GSTALIVN]-x(2)-H-E-[LIVMFYW]-{DEHRKP}-H-x-[LIVMFYWGSPQ]. NAME: Matπxins cysteine switch. CONSENSUS: P-R-C-[GN]-x-P-[DR]-[LIVSAPKQ] NAME: Insuhnase family, zinc-binding region signamre CONSENSUS G-x(8,9)-G-x-[STA]-H-[LIVMFY]-[LrVMC]-[DERN]-[HRKL]-[LMFAT]-x-[LFSTH]-x- CONSENSUS [GSTAN]-[GST]. // AC PS01016; DE Glycoprotease family signamre CONSENSUS: [KR]-[GSAT]-x(4)-[FYWHL]-[DQNGK]-x-P-x-[LIVMFY]-x(3)-H-x(2)-[AG]-H- CONSENSUS: [LIVM]. NAME: Proteasome A-type subunits signamre CONSENSUS: [FY]-x(4)-[STNV]-x-[FYW]-S-P-x-G-[RKH]-x(2)-Q-[LIVM]-[DE]-Y-[SAD]-x(2)- CONSENSUS: [SAG]. NAME Proteasome B-type subunits signamre CONSENSUS [LIVMA]-[GSA]-[LIVMF]-x-[FYLVGAC]-x(2)-[GSACFY]-[LIVMSTAC](3)-[GAC]- CONSENSUS [GSTACV]-[DES]-x(15)-[RK]-x(12,13)-G-x(2)-[GSTA]-D NAME Signal peptidases I serine active site CONSENSUS [GS]-x-S-M-x-[PS]-[AT]-[LF] NAME Signal peptidases I lysine active site CONSENSUS K-R-[LIVMSTA](2)-G-x-[PG]-G-[DE]-x-[LIVM]-x-[LIVMFY] NAME Signal peptidases I signamre 3 CONSENSUS [LIVMFYW](2)-x(2)-G-D-[NH]-x(3)-[SND]-x(2)-[SG] NAME Signal peptidases II signamre CONSENSUS [GAF]-[GA]-[GAS]-[LIVM]-[GAS]-N-[LVMFG]-[LIVMFY]-D-R-[LIMFA] NAME Peptidase family U32 signature CONSENSUS E-x-F-x(2)-G-[SA]-[LIVM]-C-x(4)-G-x-C-x-[LIVM]-S NAME Amidases signamre CONSENSUS G-[GA]-S-S-[GS]-G-x-[GSA]-[GSAVY]-x-[LIVM]-[GSA]-x(6)-[GSA]-x-[GA]-x-D- CONSENSUS x-[GA]-x-S-[LIVM]-R-x-P-[GSAC] NAME Asparaginase / glutaminase active site signamre 1 CONSENSUS [LIVM]-x(2)-T-G-G-T-[IV]-[AGS] NAME Asparaginase / glutaminase active site signamre 2 CONSENSUS G-x-[LIVM]-x(2)-H-G-T-D-T-[LIVM] NAME Urease mckel ligands signamre CONSENSUS T-[AY]-[GA]-[GAT]-[LIVM]-D-x-H-[LIVM]-H-x(3)-P NAME Urease active site CONSENSUS [LIVM](2)-[CT]-H-[HN]-L-x(3)-[LrVM]-x(2)-D-[LIVM]-x-F-A NAME ArgE / dapE / ACY1 / CPG2 / yscS family signamre 1 CONSENSUS [LIV]-[GALMY]-[LIVMF]-x-[GSA]-H-x-D-[TV]-[STAV] NAME ArgE / dapE / ACY1 / CPG2 / yscS family signamre 2 CONSENSUS [GSTAI]-[SANQ]-D-x-K-[GSACN]-x(2)-[LIVMA]-x(2)-[LIVMFY]-x(14,17)-[LIVM]- CONSENSUS x-[LrVMF]-[LIVMSTAG]-[LIVMFA]-x(2)-[DNG]-E-E-x-[GSTN] NAME Dihydroorotase signamre 1 CONSENSUS D-[LIVMFYWSAP]-H-[LIVA]-H-[LIVF]-[RN]-x-[PGN] NAME Dihydroorotase signamre 2 CONSENSUS [GA]-[ST]-D-x-A-P-H-x(4)-K NAME Beta-lactamase class-A active site CONSENSUS [FY]-x-[LIVMFY]-x-S-[TV]-x-K-x(4)-[AGLM]-x(2)-[LC] NAME Beta-lactamase class-C active site CONSENSUS F-E-[LIVM]-G-S-[LIVMG]-[SA]-K NAME Beta-lactamase class-D active site CONSENSUS [PA]-x-S-[ST]-F-K-[LIV]-[PAL]-x-[STA]-[LI] NAME Beta-lactamases class B signamre 1 CONSENSUS [LI]-x-[STN]-[HN]-x-H-[GSTA]-D-x(2)-G-[GP]-x(7,8)-[GS] NAME Beta-lactamases class B signature 2 CONSENSUS P-x(3)-[LIVM](2)-x-G-x-C-[LIVMF](2)-K NAME Argmase family signamre 1 CONSENSUS [LIVMF]-G-G-x-H-x-[LIVMT]-[STAV]-x-[PAG]-x(3)-[GSTA] NAME Arginase family signamre 2 CONSENSUS [LIVM](2)-x-[LIVMFY]-D-[AS]-H-x-D NAME Arginase family signamre 3 CONSENSUS [ST]-[LrVMFY]-D-[LIVM]-D-x(3)-[PAQ]-x(3)-P-[GSA]-x(7)-G NAME Adenosine and AMP deaπunase signamre CONSENSUS [SA]-[LIVM] [NGS]-[STA]-D-D-P NAME Cytidine and deoxycytidylate deaminases zinc-binding region signature CONSENSUS [CH]-[AGV]-E-x(2)-[LIVMFGAT]-[LIVM]-x(17,33)-P-C-x(2,8)-C-x(3)-[LIVM] NAME GTP cyclohydrolase I signamre 1 CONSENSUS [EN]-[LIVM](2)-x(2)-[KRQN]-[DN]-[LIVM]-x(3)-[ST]-x-C-E-H-H NAME GTP cyclohydrolase I signamre 2 CONSENSUS [SA]-x-[RK]-x-Q-[LIVM]-Q-E-[RN]-[LI]-[TSN] NAME Nitnlases / cyanide hydratase signamre 1 CONSENSUS G-x(2)-[LIVMFY](2)-x-[IF]-x-E-x(2)-[LIVM]-x-G-Y-P NAME Nitnlases / cya de hydratase active site signamre CONSENSUS G-[GAQ]-x(2)-C-[WA]-E-[NH]-x(2)-[PST]-[LIVMFYS]-x-[KR] NAME Inorganic pyrophosphatase signamre CONSENSUS D-[SGDN]-D-[PE]-[LIVMF]-D-[LIVMGAC] NAME Acylphosphatase signamre 1 CONSENSUS [LIV]-x-G-x-V-Q-G-V-x-[FM]-R NAME Acylphosphatase signature 2 CONSENSUS G-[FYW]-[AVC]-[KRQAM]-N-x(3)-G-x-V-x(5)-G NAME ATP synthase alpha and beta subunits signamre CONSENSUS P-[SAP]-[LIV]-[DNH]-x(3)-S-x-S NAME ATP synthase gamma subunit signature CONSENSUS [IV]-T-x-E-x(2)-[DE]-x(3)-G-A-x-[SAKR] NAME ATP synthase delta (OSCP) subunit signamre CONSENSUS [LIVM]-x-[LiVMFYT]-x(3)-[LIVMT] [DENQK]-x(2)-[LIVM]-x-[GSA]-G-[LIVMFYGA]- CONSENSUS x-[LIVM]-[KRHENQ]-x-[GSEN] NAME ATP synthase a subumt signamre CONSENSUS [STAGN]-x-[STAG]-[LIVMF]-R-L-x-[SAGV]-N-[LIVMT] NAME ATP synthase c subunit signamre CONSENSUS [GSTA]-R-[NQ]-P-x(10)-[LIVMFYW](2)-x(3)-[LrVMFYW]-x-[DE] NAME E1-E2 ATPases phosphorylation site CONSENSUS D-K-T-G-T-[LI]-[TI] NAME Sodium and potassium ATPases beta subunits signamre 1 CONSENSUS [FY ]-x(2)-[FYW]-x-[FYW]-[DN]-x(6)-[LIVM]-G-R-T-x(3)-W NAME Sodium and potassium ATPases beta subunits signamre 2 CONSENSUS [RK]-x(2)-C-[RKQWI]-x(5)-L-x(2)-C-[SA]-G NAME GDA1/CD39 family of nucleoside phosphatases signamre CONSENSUS [LrVM]-x-G-x(2)-E-G-x-[FY]-x-[FW]-[LIVA]-[TAG]-x-N-[HY] NAME Iodothyronine deiodinases active site CONSENSUS R-P-L-V-x-N-F-G-S-[CA]-T-C-P-x-F NAME Cutinase, senne active site CONSENSUS P-x-[STA]-x-[LIV]-[IVT]-x-[GS]-G-Y-S-[QL]-G NAME Cunnase, aspartate and histidine active sites CONSENSUS C-x(3)-D-x-[IV]-C-x-G-[GST]-x(2)-[LIVM]-x(2,3)-H NAME DDC / GAD / HDC / TyrDC pyndoxal-phosphate attachment site CONSENSUS S-[LIVMFYW]-x(5)-K-[LrVMFYWG](2)-x(3)-[LIVMFYW]-x-[CA]-x(2)-[LIVMFYWQ]- CONSENSUS x(2)-[RK] NAME Orn Lys/Arg decarboxylases family 1 pyndoxal-P attachment site CONSENSUS [STAV]-x-S-x-H-K-x(2)-[GSTAN](2)-x-[STA]-Q-[STA](2) NAME Orn/DAP/Arg decarboxylases family 2 pyndoxal-P attachment site CONSENSUS [FY]-[PA]-X-K-[SACV]-[NHCLFW]-X(4)-[LIVMF]-[LIVMTA]-X(2)-[LΓVMA]-X(3)- CONSENSUS [GTE] NAME Orn DAP/Arg decarboxylases family 2 signamre 2 CONSENSUS [GS]-x(2,6)-[LIVMSCP]-x(2)-[LIVMF]-[DNS]-[LIVMCA]-G-G-G-[LIVMFY]- CONSENSUS [GSTPCEQ] NAME Orotidine 5 '-phosphate decarboxylase active site CONSENSUS [LIVMFTA]-[LIVMF]-x-D-x-K-x(2)-D-I-[GP]-x-T-[LIVMTA] NAME Phosphoenolpyruvate carboxylase active site 1 CONSENSUS [VT]-x-T-A-H-P-T-[EQ]-x(2)-R-[KRH] NAME Phosphoenolpyruvate carboxylase active site 2 CONSENSUS [IV]-M-[LIVM]-G-Y-S-D-S-x-K-D-[STAG]-G NAME Phosphoenolpyruvate carboxykinase (GTP) signamre CONSENSUS F-P-S-A-C-G-K-T-N NAME Phosphoenolpyruvate carboxykinase (ATP) signamre CONSENSUS L-I-G-D-D-E-H-x-W-x-[DE]-x-G-[IV]-x-N NAME Uroporphyπnogen decarboxylase signamre 1 CONSENSUS P-x-W-x-M-R-Q-A-G-R NAME Uroporphyπnogen decarboxylase signamre 2 CONSENSUS G-F-[STAGCV]-[STAGC]-x-P-[FYW]-T-[LV]-x(2)-Y-x(2)-[AE]-[GK] NAME Indole-3-glycerol phosphate synthase signamre CONSENSUS [LIVMFY]-[LΓVMC]-X-E-[LIVMFYC]-K-[KRSP]-[STAK]-S-P-[ST]-X(3)-[LΓVMFYST] NAME Ribulose bisphosphate carboxylase large chain active site CONSENSUS G-x-[DN]-F-x-K-x-D-E NAME Fructose-bisphosphate aldolase class-I active site CONSENSUS [LIVM]-x-[LIVMFYW]-E-G-x-[LS]-L-K-P-[SN] NAME Fructose-bisphosphate aldolase class-II signamre 1 CONSENSUS [FYVM]-x(l,3)-[LIVMH]-[APN]-[LIVM]-x(l,2)-[LIVM]-H-x-D-H [GACH] NAME Fructose-bisphosphate aldolase class-II signamre 2 CONSENSUS [LIVM]-E-x-E-[LIVM]-G-x(2)-[GM]-[GSTA]-x-E NAME Malate synthase signamre CONSENSUS [KR]-[DENQ]-H-x(2)-G-L-N-x-G-x-W-D-Y-[LIVM]-F NAME Hydroxymethylglutaryl-coenzyme A lyase active site CONSENSUS S-V-A-G-L-G-G-C-P-Y NAME Hydroxymethylglutaryl-coenzyme A synthase active site CONSENSUS N-x-[DN]-[rV]-E-G-[IV]-D-x(2)-N-A-C-[FY]-x-G NAME Citrate synthase signature CONSENSUS G-[FYA]-[GA]-H-x-[IV]-x(l,2)-[RKT]-x(2)-D-[PS]-R NAME Alpha-isopropylmalate and homocitrate synthases signamre 1 CONSENSUS L-R-[DE]-G-x-Q-x(10)-K NAME Alpha-isopropylmalate and homocitrate synthases signamre 2 CONSENSUS [LIVMFW]-x(2)-H-x-H-[DN]-D-x-G-x-[GAS]-x-[GASLI] NAME KDPG and KHG aldolases active site CONSENSUS G-[LIVM]-x(3)-E-[LIV]-T-[LF]-R NAME KDPG and KHG aldolases Schiff-base forming residue CONSENSUS G-x(3)-[LIVMF]-K-[LF]-F-P-[SA]-x(3)-G NAME Isocitrate lyase signamre CONSENSUS K-[KR]-C-G-H-[LMQ] NAME Beta-eliminating lyases pyndoxal-phosphate attachment site CONSENSUS Y-x-D-x(3)-M-S-[GA]-K-K-D-x-[LIVM](2)-x-[LIVM]-G-G NAME DNA photolyases class 1 signamre 1 CONSENSUS T-G-x-P-[LIVM](2)-D-A-x-M-[RA]-x-[LIVM] NAME DNA photolyases class 1 signamre 2 CONSENSUS [DN]-R-x-R-[LIVM](2)-x-[STA](2)-F-[LIVMFA]-x-K-x-L-x(2,3)-W-[KRQ] NAME DNA photolyases class 2 signamre 1 CONSENSUS F-x-E-E-x-[LIVM](2)-R-R-E-L-x(2)-N-F NAME DNA photolyases class 2 signamre 2 CONSENSUS G-x-H-D-x(2)-W-x-E-R-x-[LIVM]-F-G-K-[LIVM]-R-[FY]-M-N NAME Eukaryotic-type carbonic anhydrases signamre CONSENSUS S-E-H-x-[LIVM]-x(4)-[FYH]-x(2)-E-[LIVM]-H-[LIVMFA](2) NAME Prokaryotic-type carbonic anhydrases signamre 1 CONSENSUS C-[SA]-D-S-R-[LIVM]-x-[AP] NAME Prokaryotic-type carbomc anhydrases signature 2 CONSENSUS [EQ]-Y-A-[LIVM]-x(2)-[LIVM]-x(4)-[LIVMF](3)-x-G-H-x(2)-C-G Fumarate lyases signamre CONSENSUS G-S-x(2)-M-x(2)-K-x-N NAME Acomtase family signamre 1 CONSENSUS [LIVM]-x(2)-[GSACIVM]-x-[LIV]-[GTIV]-[STP]-C-x(0,l)-T-N-[GSTANI]-x(4)- CONSENSUS [LIVMA] NAME Acomtase family signamre 2 CONSENSUS G-x(2)-[LIVWPQ]-x(3)-[GAC]-C-[GSTAM]-[LIMPTA]-C-[LIMV]-[GA] NAME Dihydroxy-acid and 6-phosphogluconate dehydratases signamre 1 CONSENSUS C-D-K-x(2)-P-[GA]-x(3)-[GA] NAME Dihydroxy-acid and 6-phosphogluconate dehydratases signamre 2 CONSENSUS [SA]-L-[LIVM]-T-D-[GA]-R-[LIVMF]-S-[GA]-[GAV]-[ST] NAME Dehydroquinase class I active site CONSENSUS D-[LIVM]-[DE]-[LIVN]-x(18,20)-[LIVM](2)-x-[SC]-[NHY]-H-[DN] NAME Dehydroquinase class II signature CONSENSUS [LIVM]-[NQ]-G-P-N-[LV]-x(2)-L-G-x-R-[QED]-P-x(2)-[FY]-G NAME Enolase signamre CONSENSUS [LIV](3)-K-x-N-Q-I-G-[ST]-[LIV]-[ST]-[DE]-[STA] NAME Senne/threonine dehydratases pyndoxal-phosphate attachment site CONSENSUS [DESH]-x(4,5)-[STVG]-x-[AS]-[FYI]-K-[DLIFSA]-[RVMF]-[GA]-[LIVMGA] NAME Enoyl-CoA hydratase/isomerase signamre CONSENSUS [LIVM]-[STA]-X-[LIVM]-[DENQRHSTA]-G-X(3)-[AG](3)-X(4)-[LIVMST]-X-[CSTA]- CONSENSUS [DQHPHLΓVMFY] NAME Imidazoleglycerol-phosphate dehydratase signamre 1 CONSENSUS [LΓVMY]-[DE]-X-H-H-X(2)-E-X(2)-[GCA]-[LIVM]-[STAC]-[LIVM] NAME Imidazoleglycerol-phosphate dehydratase signamre 2 CONSENSUS G-x-[DN]-x-H-H-x(2)-E-[STAGC]-x-[FY]-K NAME Tryptophan synthase alpha chain signamre CONSENSUS [LIVM]-E-[LIVM]-G-x(2)-[FYC]-[ST]-[DE}-[PA]-[LIVMY]-[AGLη-[DE]-G NAME Tryptophan synthase beta chain pyndoxal-phosphate attachment site CONSENSUS [LIVM]-x-H-x-G-[STA]-H-K-x-N NAME Delta-aminolevulinic acid dehydratase active site CONSENSUS G-x-D-x-[LIVM](2)-[IV]-K-P-[GSA]-x(2)-Y NAME Urocanase active site CONSENSUS F-Q-G-L-P-x-R-I-C-W NAME Prephenate dehydratase signamre 1 CONSENSUS [Fι^-x-[LIV^-x(2)-[LIVM]-x(5)-PN]-x(5)-T-R-F-[LIVM ]-x-[LIVM] NAME Prephenate dehydratase signamre 2 CONSENSUS [LIVM]-[ST]-[KR]-[LIVM]-E-[ST]-R-P NAME Dihydrodipicolinate synthetase signamre 1 CONSENSUS [GSA]-[LIVM]-[LIVMFY]-x(2)-G-[ST]-[TG]-G-E-[GASNF]-x(6)-[EQ] NAME Dihydrodipicolinate synthetase signamre 2 CONSENSUS Y-[DNS]-[LIVMF]-P-x(2)-[ST]-x(3)-[LIVM]-x(13, 14)-[LIVM]-x-[SGA]-[LIVMF]- CONSENSUS K-[DEQAF]-[STAC] NAME RsuA family of pseudouπdine synthase signamre CONSENSUS G-R-L-D-x(2)-[ST]-x-G-[LIVMF](4)-[ST]-[DNT] NAME Cysteine synthase/cystathiomne beta-synthase P-phosphate attachment site CONSENSUS K-x-E-x(3)-[PA]-[STAGC]-x-S-[IVAP]-K-x-R-x-[STAG]-x(2)-[LIVM] NAME Phenylalanine and histidine ammonia-lyases signamre CONSENSUS G-[STG]-[LIVM]-[STG]-[AC]-S-G-[DH]-L-x-P-L-[SA]-x(2)-[SA] NAME Porphobihnogen deaminase cofactor-binding site CONSENSUS E-R-x-[LIVMFA]-x(3)-[LIVMF]-x-G-[GSA]-C-x-[IVT]-P-[LIVMF]-[GSA] NAME Cys/Met metabolism enzymes pyndoxal-phosphate attachment site CONSENSUS [DQ]-[LIVMF]-x(3)-[STAGC]-[STAGCI]-T-K-[FYWQ]-[LIVMF]-x-G-[HQ]-[SGNH] NAME Glyoxalase I signamre 1 CONSENSUS [HQ]-[IVT]-x-[LIVFY]-x-[IV]-x(5)-[STA]-x(2)-F-[YM]-x(2,3)-[LMF]-G-[LMF] NAME Glyoxalase I signamre 2 CONSENSUS G-[NTKQ]-x(0,5)-[GA]-[LVFY]-[GH]-H-[IVF]-[CGA]-x-[STAGL]-x(2)-[DNC] NAME Cytochrome c and cl heme lyases signamre 1 CONSENSUS H-N-x(2)-N-E-x(2)-W-[NQKR]-x(4)-W-E Cytochrome c and c 1 heme lyases signamre 2 CONSENSUS P-F-D-R-H-D-W NAME Adenylate cyclases class-I signature 1 CONSENSUS E-Y-F-G-[SA](2)-L-W-x-L-Y-K NAME Adenylate cyclases class-I signamre 2 CONSENSUS Y-R-N-x-W-[NS]-E-[LIVM]-R-T-L-H-F-x-G NAME Guanylate cyclases signamre CONSENSUS G-V-[LIVM]-x(0,l)-G-x(5)-[FY]-x-[LIVM]-[FYW]-[GS]-[DNTHKW]-[DNT]-[IV]- CONSENSUS [DNTA]-x(5)-[DE] NAME Chons ate synthase signamre 1 CONSENSUS G-E-S H [GC]-x(2)-[LIVM]-[GTV]-x-[LIVM](2)-[DE]-G-x-[PV] NAME Chonsmate synthase signature 2 CONSENSUS [GE]-R-[SA](2)-[SAG]-R-[EV]-[ST]-x(2)-[RH]-V-x(2)-G NAME Chonsmate synthase signamre 3 CONSENSUS R-[SH]-D-[PSV]-[CSAV]-x(4)-[GAI]-x-[rVGSP]-[LIVM]-x-E-[STAH]-[LIVM] NAME 6-pyruvoyl tetrahydropteπn synthase signamre 1 CONSENSUS C-N-N-x(2)-G-H-G-H-N-Y NAME 6-pyruvoyl tetrahydropteπn synthase signamre 2 CONSENSUS D-H-K-N-L-D-x-D NAME Ferrochelatase signamre CONSENSUS [LIVMF](2)-x-S-x-H-[GS]-[LIVM]-P-x(4,5)-[DENQKR]-x-G-D-x-Y NAME Alamne racemase pyndoxal-phosphate attachment site CONSENSUS V-x-K-A-[DN]-[GA]-Y-G-H-G NAME Aspartate and glutamate racemases signature 1 CONSENSUS [T A]-[LiVM]-x-C-x(0, 1)-N-[ST]-[MSA]-[STH]-[LIVFYSTANK] NAME Aspartate and glutamate racemases signamre 2 CONSENSUS [LIVM](2)-x-[AG]-C-T-[DEH]-[LIVMFY]-[PNGRS]-x-[LIVM] NAME Mandelate racemase / muconate lactomzing enzyme family signamre 1 CONSENSUS A-x-[SAG](2)-[LIVM]-[DE]-x-A-x(2)-D-x(2)-[GA]-[KR] NAME Mandelate racemase / muconate lactomzing enzyme family signamre 2 CONSENSUS G-x(7)-D-x(9)-A-x(14)-[LIVM]-E-[DENQ]-P-x(4)-[DENQ] NAME Ribulose-phosphate 3-epιmerase family signamre 1 CONSENSUS [LIVMF]-H-[LIVMFY]-D-[LIVM]-x-D-x( 1 ,2)-[FY]-[LIVM]-x-N-x-[STAV] NAME Ribulose-phosphate 3-epιmerase family signature 2 CONSENSUS [LIVMA]-x-[LIVM]-M-[ST]-[VS]-x-P-x(3)-G-Q-x-F-x(6)-[NK]-[LIVMC] NAME Aldose 1-epιmerase putative active site CONSENSUS [NS]-x-T-N-H-x-Y-[FW]-N-[LI] NAME Cyclophihn-type peptidyl-prolyl cis-trans isomerase signature CONSENSUS [FY]-x(2)-[STCNLV]-x-F-H-[RH]-[LIVMN]-[LIVM]-x(2)-F-[LIVM]-x-Q-[AG]-G NAME Cyclophihn-type peptidyl-prolyl cis-trans isomerase profile NAME FKBP-type peptidyl-prolyl cis-trans isomerase signamre 1 CONSENSUS [LIVMC]-x-[YF]-x-[GVL]-x( 1 ,2)-[LFT]-x(2)-G-x(3)-[DE]-[STAEQK]-[STAN] NAME FKBP-type peptidyl-prolyl cis-trans isomerase signamre 2 CONSENSUS [LIVMFY]-x(2)-[GA]-x(3 ,4)-[LIVMF]-x(2) [LIVMFHK]-x(2)-G-x(4)-[LIVMF]- CONSENSUS x(3)-[PSGAQ]-x(2)-[AG]-[FY]-G NAME FKBP-type peptidyl-prolyl cis-trans isomerase domain profile NAME PpiC-type peptidyl-prolyl cis-trans isomerase signamre CONSENSUS F-[GSADEI]-x-[LVAQ]-A-x(3)-[ST]-x(3,4)-[STQ]-x(3,5)-[GER]-G-x-[LrVM]- CONSENSUS [GS] NAME Tπosephosphate isomerase active site CONSENSUS [AV]-Y-E-P-[LIVM]-W-[SA]-I-G-T-[GK] NAME Xylose isomerase signamre 1 CONSENSUS [LI]-E-P-K-P-x(2)-P NAME Xylose isomerase signamre 2 CONSENSUS [FL]-H-D-x-D-[LIV]-x-[PD]-x-[GDE] NAME Phosphomannose isomerase type I signamre 1 CONSENSUS Y-x-D-x-N-H-K-P-E NAME Phosphomannose isomerase type I signamre 2 CONSENSUS H-A-Y-[LIVM]-x-G-x(2)-[LIVM]-E-x-M-A-x-S-D N-x-[LIVM]-R-A-G-x-T-P-K NAME Phosphoglucose isomerase signamre 1 CONSENSUS [DENS]-x-[LIVM]-G-G-R-[FY]-S-[LIVMT]-x-[STA]-[PSAC]-[LIVMA]-G NAME Phosphoglucose isomerase signamre 2 CONSENSUS [GS]-x-[LIVM]-[LIVMFYW]-x(4)-[FY]-[DN]-Q-x-G-V-E-x(2)-K NAME Glucosamιne/galactosamιne-6-phosphate isomerases signamre CONSENSUS [LIVM]-x(3)-G-x-[LIT]-x-[LIV]-x-[LIVM]-x-G-[LIVM]-G-x-[DEN]-G-H NAME Phosphoglycerate mutase family phosphohistidme signamre CONSENSUS [LIVM]-x-R-H-G-[EQ]-x(3)-N NAME Phosphoglucomutase and phosphomannomutase phosphoseπne signamre CONSENSUS [GSA]-[LIVM]-x-[LIVM]-[ST]-[PGA]-S-H-x-P-x(4)-[GNHE] NAME Methylmalonyl-CoA mutase signamre CONSENSUS R-I-A-R-N-[TQ]-x(2)-[LIVMFY](2)-x-[EQ]-E-x(4)-[KRN]-x(2)-D-P-x-[GSA]- CONSENSUS G-S NAME Terpene synthases signamre CONSENSUS [DE]-G-S-W-x-G-x-W-[GA]-[LIVM]-x-[FY]-x-Y-[GA] NAME Eukaryonc DNA topoisomerase I active site CONSENSUS [DEN]-x(6)-[GS]-[IT]-S-K-x(2)-Y-[LIVM]-x(3)-[LIVM] NAME Prokaryonc DNA topoisomerase I active site CONSENSUS [EQ]-x-L-Y-[DEQT]-x(3, 12)-[LI]-[ST]-Y-x-R-[ST]-[DEQS] NAME DNA topoisomerase II signamre CONSENSUS [LIVMA]-x-E-G-[DN]-S-A-x-[STAG] NAME: Aminoacyl-transfer RNA synthetases class-I signamre. CONSENSUS: P-x(0,2)-[GSTAN]-[DENQGAPK]-x-[LIVMFP]-[HT]-[LIVMYAC]-G-[HNTG]- CONSENSUS: [LIVMFYSTAGPC]. NAME: Aminoacyl-transfer RNA synthetases class-II signamre 1. CONSENSUS: [FYH]-R-x-[DE]-x(4,12)-[RH]-x(3)-F-x(3)-[DE]. NAME: Aminoacyl-transfer RNA synthetases class-II signamre 2 CONSENSUS: [GSTALVF]-{DENQHRKP}-[GSTA]-[LIVMF]-[DE]-R-[LIVMF]-x-[LIVMSTAG]-[LIVMFY]. NAME: WHEP-TRS domain signamre. CONSENSUS: [QY]-G-[DNEA]-x-[LIV]-[KR]-x(2)-K-x(2)-[KRNG]-[AS]-x(4)-[LIV]-[DENK]- CONSENSUS: x(2)-[IV]-x(2)-L-x(3)-K. NAME: ATP-citrate lyase / succinyl-CoA hgases family signamre 1. CONSENSUS: S-[KR]-S-G-[GT]-[LIVM]-[GST]-x-[EQ]-x(8,10)-G-x(4)-[LIVM]-[GA]-[LIVM]-G- CONSENSUS: G D. NAME: ATP-citrate lyase / succmyl-CoA hgases family active site. CONSENSUS: G-x(2)-A-x(4,7)-[RQT]-[LIVMF]-G-H-[AS]-[GH] . NAME: ATP-citrate lyase / succinyl-CoA hgases family signamre 3. CONSENSUS: G-x-[IV]-x(2)-[LIVMF]-x-[NA]-G-[GA]-G-[LA]-[STAV]-x(4)-D-x-[LIVM]-x(3)- CONSENSUS: G-[GRE]. NAME: Glutamine synthetase signamre 1. CONSENSUS: [FYWL]-D-G-S-S-x(6,8)-[DENQSTAK]-[SA]-[DE]-x(2)-[LIVMFY]. NAME: Glutamine synthetase putanve ATP-bmding region signamre. CONSENSUS: K-P-[LIVMFYA]-x(3,5)-[NPAT]-G-[GSTAN]-G-x-H-x(3)-S. NAME: Glutamine synthetase class-I adenylation site. CONSENSUS: K-[LIVM]-x(5)-[LIVMA]-D-[RK]-[DN]-[LI]-Y. NAME: D-alamne-D-alamne ligase signamre 1. CONSENSUS: H-G-x(2)-G-E-D-G-x-[LIVMA]-[QSA]-[GSA]. NAME: D-alamne-D-alamne ligase signature 2. CONSENSUS: [LIV]-x(3)-[GA]-x-[GSAIV]-R-[LIVCA]-D-[LIVMF](2)-x(7,9)-[LI]-x-E- CONSENSUS: [LIVA]-N-[STP]-x-P-[GA] . NAME: SAICAR synthetase signamre 1. CONSENSUS: [LIVMF](2)-P-[LIVM]-E-x-[LIVM]-[LIVMCA]-R-x(3)-[TA]-G-S. N.AME: SAICAR synthetase signamre 2. CONSENSUS: [LIVM]-[LrVMA]-D-x-K-[LIVMFY]-E-F-G. NAME: Folylpolyglutamate synthase signamre 1. CONSENSUS: [LIVMFY]-x-[LιVM]-[STAG]-G-T-[NK]-G-K-x-[ST]-x(7)-[LIVM](2)-x(3)-[GSK]. NAME: Folylpolyglutamate synthase signamre 2. CONSENSUS: [LIVMFY](2)-E-x-G-[LIVM]-[GA]-G-x(2)-D-x-[GST]-x-[LIVM](2). NAME: Ubiquitin-activating enzyme signamre 1. CONSENSUS: K-A-C-S-G-K-F-x-P. NAME: Ubiquitin-acnvating enzyme active site. CONSENSUS: P-[LrVM]-C-T-[LIVM]-[KRH]-x-[FT]-P. NAME: Ubiquitin-conjugating enzymes active site. CONSENSUS: [FYWLSP]-H-[PC]-[NH]-[LIV]-x(3,4)-G-x-[LIV]-C-[LIV]-x-[LIV]. NAME: Formate-tetrahydrofolate ligase signamre 1. CONSENSUS: G-[LιVM]-K-G-G-A-A-G-G-G-Y. NAME: Formate-tetrahydrofolate ligase signamre 2. CONSENSUS: V-A-T-[IV]-R-A-L-K-x-[HN]-G-G. NAME: Adenylosucc ate synthetase GTP-binding site. CONSENSUS: Q-W-G-D-E-G-K-G. NAME: Adenylosuccmate synthetase active site. CONSENSUS: G-I-[GR]-P-x-Y-x(2)-K-x(2)-R. NAME Argininosuccinate synthase signature 1 CONSENSUS A-[FY]-S-G-G-L-D-T-S NAME Argininosuccinate synthase signamre 2 CONSENSUS G-x-T-x-K-G-N-D-x(2)-R-F NAME Phosphoπbosylglycinamide synthetase signamre CONSENSUS R-F-G-D-P-E-x-[QM] NAME Carbamoyl-phosphate synthase subdomain signamre 1 CONSENSUS [FYV]-[PS]-[LIVMC]-[LrVMA]-[LIVM]-[KR]-[PSA]-[STA]-x(3)-[SG]-G-x-[AG] NAME Carbamoyl-phosphate synthase subdomain signamre 2 CONSENSUS [LIVMF]-[LIMN]-E-[LIVMCA]-N-[PATLIVM]-[KR]-[LIVMSTAC] NAME ATP-dependent DNA ligase AMP-bmding site CONSENSUS [EDQH]-x-K-x-[DN]-G-x-R-[GACIVM] NAME ATP-dependent DNA ligase signamre 2 CONSENSUS E-G-[LIVMA]-[LIVM](2)-[KR]-x(5,8)-[YW]-[QNEK]-x(2,6)-[KRH]-x(3,5)-K- CONSENSUS [LIVMFY]-K NAME NAD-dependent DNA ligase signamre 1 CONSENSUS K-[LIVM]-D-G-[LIVM]-[SA]-x(4)-Y-x(2)-G-x-L-x(4)-[ST]-R-G-[DN]-G-x(2)-G- CONSENSUS [DEHDENL] NAME NAD-dependent DNA ligase signamre 2 CONSENSUS [IV]-G-[KR]-[ST]-G-x-[LIVM] [STNK]-x-[VT]-x(2)-L-x-[PS]-V NAME RNA 3' -terminal phosphate cyclase signamre CONSENSUS [RH]-G-x(2)-P-x G(3)-x-[LIV] NAME Lipoate-protein ligase B signamre CONSENSUS R-G-G-X(2)-T-[FYW]-H-X(2)-[GH]-Q-X-[LΓV]-X-Y NAME Isopenicillin N synthetase signamre 1 CONSENSUS [RK]-x-[STA]-x(2)-S-x-C-Y-[SL] NAME Isopenicillin N synthetase signamre 2 CONSENSUS [LIVM](2)-x-C-G-[STA]-x(2)-[STAG]-x(2)-T-x-[DNG] NAME Site-specific recombinases active site CONSENSUS Y-[LIVAC]-R-[VA]-S-[ST]-x(2)-Q NAME Site-specific recombinases signamre 2 CONSENSUS G-[DE]-x(2)-[LIVM]-x(3)-[LIVM]-[DT]-R-[LIVM]-[GSA] NAME Transposases, Mutator family, signamre CONSENSUS D-x(3)-G-rLrVMF]-x(6)-[STAV]-[LIVMFYW]-[PT]-x-[STAV]-x(2)-[QR]-x-C-x(2)- CONSENSUS H NAME Transposases, IS30 family, signamre CONSENSUS R-G-x(2)-E-N-x-N-G-[LIVM](2)-R-[QE]-[LIVMFY](2)-P-K NAME Autoinducers synthetases family signamre CONSENSUS [LMFY]-R-x(3)-F-x(2)-[KR]-x(2)-W-x-[LIVM]-x(6,9)-E-x-D-x-[FY]-D NAME Thiamine pyrophosphate enzymes signamre CONSENSUS [LrVMF]-[GSA]-x(5)-P-x(4)-[LIVMFYW]-x-[LIVMF]-x-G-D-[GSA]-[GSAC] NAME Biotin-requiπng enzymes attachment site CONSENSUS [GN]-[DEQTR]-x-[LIVMFY]-x(2)-[LIVM]-x-[AIV]-M-K-[LMAT]-x(3)-[LIVM]-x- CONSENSUS [SAV] NAME 2-oxo acid dehydrogenases acyltransferase component lipoyl bmding site CONSENSUS [GN]-x(2)-[LIVF]-x(5)-[LIVFC]-x(2)-[LIVFA]-x(3)-K-[STAIV]-[STAVQDN]- CONSENSUS x(2)-[LIVMFS]-x(5)-[GCN]-x-[LIVMFY] NAME Putative AMP-binding domain signamre CONSENSUS [LIVMFY]-x(2)-[STG]-[STAG]-G-[ST]-[STEI]-[SG]-x-[PASLIVM]-[KR] NAME Molybdenum cofactor biosynthesis proteins signamre 1 CONSENSUS [LIVM](3)-[LIT](2)-G-G-T-G-x(4)-D NAME: Molybdenum cofactor biosynthesis proteins signamre 2. CONSENSUS S-x-[GS]-x(2)-D-x(5)-[LIVW]-x( 10, 12)-[LIV]-X(2)-[KR]-P-G-[KRL]-P-X(2)- CONSENSUS- [LΓVMF]-[GA]. NAME: moaA / nifB / pqqE family signamre. CONSENSUS: [LIV]-x(3)-C-[NP]-[LIVMF]-[QRS]-C-x-[FYM]-C. NAME. Radical activating enzymes signamre. CONSENSUS [GV]-x-G-x-[KR]-x(3)-F-x(2)-G-x(0,l)-C-x(3)-C-x(2)-C-x-[NL] NAME Tpx family signamre. CONSENSUS: S-x-D-L-P-F-A-x(2)-[KR]-[FW]-C. NAME- Cytochrome c family heme-binding site signamre. CONSENSUS: C-{CPWHF}-{CPWR}-C-H-{CFYW}. NAME: Cytochrome b5 family, heme-binding domain signamre. CONSENSUS [FY]-[LIVMK]-x(2)-H-P-[GA]-G NAME: Cytochrome b/b6 heme- gand signature CONSENSUS. [DENQ]-x(3)-G-[FYWMQ]-x-[LIVMF]-R-x(2)-H. NAME: Cytochrome b/b6 Qo site signamre. CONSENSUS: P-[DE]-W-[FY]-[LFY](2) NAME: Cytochrome b559 subunits heme-binding site signamre. CONSENSUS: [LIV]-x-[ST]-[LIVF]-R-[FYW]-x(2)-[IV]-H-[STGA]-[LIV]-[STGA]-[IV]-P. NAME. Nickel-dependent hydrogenases b-type cytochrome subumt signamre 1 CONSENSUS R-[LIVMFYW]-X-H-W-[LΓVM]-X(2)-[LIVMF]-[STAC]-[LIVM]-X(2)-L-X-[LIVM]-T-G NAME- Nickel-dependent hydrogenases b-type cytochrome subunit signamre 2 CONSENSUS- [RH]-[STA]-[LIVMFYW]-H-[RH]-[LΓVM]-X(2)-W-X-[LIVMF]-X(2)-F-X(3)-H. NAME Succinate dehydrogenase cytochrome b subumt signamre 1 CONSENSUS. R-P-[LΓVMT]-X(3)-[LIVM]-X(6)-[LIVMWPK]-X(4)-S-X(2)-H-R-X-[ST] NAME- Succinate dehydrogenase cytochrome b subumt signamre 2 CONSENSUS H-X(3)-[GA]-[LIVMT]-R-[HF]-[LIVMF]-X-[FYWM]-D-X-[GVA]. NAME: Thioredoxin family acnve site CONSENSUS- [LIVMF]-[LIVMSTA]-X-[LIVMFYC]-[FYWSTHE]-X(2)-[FYWGTN]-C-[GATPLVE]- CONSENSUS [PHYWSTA]-C-x(6)-[LIVMFYWT] . NAME- Glutaredoxin active site. CONSENSUS: [LIVD]-[FYSA]-x(4)-C-[PV]-[FYW]-C-x(2)-[TAV]-x(2,3)-[LIV]. NAME: Type-1 copper (blue) proteins signamre CONSENSUS. [GA]-x(0,2)-[YSA]-x(0,l)-[VFY]-x-C-x(l,2)-[PG]-x(0,l)-H-x(2,4)-[MQ]. NAME: 2Fe-2S ferredoxins, iron-sulfur bmding region signamre. CONSENSUS C-{C}-{C}-[GA]-{C}-C-[GAST]-{CPDEKRHFYW}-C. NAME: Adrenodoxin family, iron-sulfur binding region signamre. CONSENSUS: C-x(2)-[STAQ]-x-[STAMV]-C-[STA]-T-C-[HR]. NAME: 4Fe-4S ferredoxins, iron-sulfur binding region signamre. CONSENSUS: C-x(2)-C-x(2)-C-x(3)-C-[PEG]. NAME: High potential iron-sulfur proteins signamre CONSENSUS: C-x(6,9)-[LIVM]-x(3)-G-[YW]-C-x(2)-[FYW]. NAME: Rieske iron-sulfur protein signamre 1. CONSENSUS. C-[TK]-H-L-G-C-[LIVT]. NAME: Rieske iron-sulfur protein signamre 2. CONSENSUS: C-P-C-H-x-[GSA] NAME: Flavodoxin signamre. CONSENSUS: [LIV]-[LIVFY]-[FY]-x-[ST]-x(2)-[AGC]-x-T-x(3)-A-x(2)-[LIV]. NAME: Rubredoxm signature CONSENSUS: [LIVM]-x(3)-W-x-C-P-x-C-[AGD]. NAME Electron transfer flavoprotein alpha-subumt signamre CONSENSUS [LI]-Y-[LIVM]-[AT]-x-G-[IV]-[SD]-G-x-[IV]-Q-H-x(2)-G-x(6)-[IV]-x-A- CONSENSUS [IV]-N NAME Electron transfer flavoprotein beta-subumt signamre CONSENSUS [IVA]-x-[KR]-x(2)-[DE]-[GD]-[GDE]-x( 1 ,2)-[EQ]-x-[LIV]-x(4)-P-x-[LIVM](2)- CONSENSUS [TAC] NAME Vertebrate metallothioneins signamre CONSENSUS C-x-C-[GSTAP]-x(2)-C-x-C-x(2)-C-x-C-x(2)-C-x-K NAME Ferπtin iron-binding regions signamre 1 CONSENSUS E-x-[KR]-E-x(2)-E-[KR]-[LF]-[LIVMA]-x(2)-Q-N-x-R-x-G-R NAME Ferπtin iron-binding regions signamre 2 CONSENSUS D-x(2)-[LIVMF]-[STAC]-[DH]-F-[LI]-[EN]-x(2)-[FY]-L-x(6)-[LIVM]-[KN] NAME Bacteπoferπtm signature CONSENSUS < M-x-G-x(3)-V-[LIV]-x(2)-[LM]-x(3)-L-x(3)-L NAME Transfernns signature 1 CONSENSUS Y-x(0, 1)-[VAS]-V-[IVAC]-[IVA]-[IVA]-[RKH]-[RKS]-[GDENSA] NAME Transfernns signamre 2 CONSENSUS Y-x-G-A-[FL]-[KRHNQ]-C-L-x(3,4)-G-[DENQ]-V-[GA]-[FYW] NAME Transfernns signamre 3 CONSENSUS [DENQ]-[YF]-x-[LY]-L-C-x-[DN]-x(5,8)-[LIV]-x(4,5)-C-x(2)-A-x(4)-[HQR]-x- CONSENSUS [LIVMFYWHLIVM] NAME Globins profile NAME Protozoan cyanobactenal globins signamre CONSENSUS F-[LF]-x(5)-G-[PA]-x(4)-G-[KRA]-x-[LIVM]-x(3)-H NAME Plant hemoglobins signamre CONSENSUS [SN]-P-x-L-x(2)-H-A-x(3)-F NAME Hemerythnns signamre CONSENSUS W-L-x-[NQ]-H-I-x(3)-D-F NAME Arthropod hemocyanins / insect LSPs signamre 1 CONSENSUS Y [FYW]-x-E-D-[LIVM]-x(2)-N-x(6)-H-x(3)-P NAME Arthropod hemocyanins / insect LSPs signamre 2 CONSENSUS T-x(2)-R-D-P-x-[FY]-[FYW] NAME Heavy-metal-associated domain CONSENSUS [LIVN]-x(2)-[LIVMFA]-x-C-x-[STAGCDNH]-C-x(3)-[LIVFG]-x(3)-[LIV]-x(9, 11 )- CONSENSUS [IVA]-x-[LVFYS] NAME ABC transporters family signamre CONSENSUS [LIVMFYC]-[SA]-[SAPGLVFYKQH]-G-[DENQMW]-[KRQASPCLIMFW]-[KRNQSTAVM]- CONSENSUS [KRACLVM]-[LΓVMFYPAN]-{PHY}-[LΓVMFW]-[SAGCLIVP]-{FYWHP}-{KRHP}- CONSENSUS [LIVMFYWSTA] NAME Binding-protein-dependent transport systems inner membrane comp sign CONSENSUS [LIVMFY]-x(8)-[EQR]-[STAGV]-[STAG]-x(3)-G-[LIVMFYSTAC]-x(5)-[LIVMFYSTA]- CONSENSUS x(4)-[LIVMFY]-[PKR] NAME ABC-2 type transport system integral membrane proteins signamre CONSENSUS [LIMST]-x(2)-[L]MW]-x(2)-[LIMCA]-[GSTC]-x-[GSAIV]-x(6)-[LIMGA]-[PGSNQ]- CONSENSUS x(9, 12)-P-[LIMFT]-x-[HRSY]-x(5)-[RQ] NAME Bacterial extracellular solute-binding proteins, family 1 signamre CONSENSUS [GAP]-[LIVMFA]-[STAVDN]-x(4)-[GSAV]-[LIVMFY](2)-Y-[ND]-x(3)-[LIVMF]-x- CONSENSUS [KNDE] NAME Bacterial extracellular solute-binding proterns, family 3 signamre CONSENSUS G-[FYIL]-[DE]-[LIVMT]-[DE]-[LIVMF]-x(3)-[LIVMA]-rVAGC]-x(2)-[LrVMAGN] NAME Bacterial extracellular solute-binding proterns, family 5 signamre CONSENSUS [AG]-X(6,7)-[DNEG]-X(2)-[STAVE]-[LIVMFYWA]-X-[LIVMFY]-X-[LΓVM]-[KR]- CONSENSUS [KRHDE]-[GDN]-[LIVMA]-[KNGSP]-[FW] NAME Serum albumin family signamre CONSENSUS [FY]-x(6)-C-C-x(7)-C-[LFY]-x(6)-[LIVMFYW] NAME Transthyretin signamre 1 CONSENSUS S-K-C-P-L-M-V-K-V-L-D-[AS]-V-R-G NAME Transthyretin signamre 2 CONSENSUS S-P-[FY]-S-[FY]-S-T-T-A-[LIVM]-V-[ST]-x-P NAME Avidin / Streptavidin family signamre CONSENSUS [DEN]-x(2)-[KR]-[STA]-x(2)-V-G-x-[DN]-x-[F ]-T-[KR] NAME Eukaryotic cobalamin-binding proteins signamre CONSENSUS [SN]-V-D-T-[GA]-A-[LIVM]-A-x-L-A-[LIVMF]-T-C NAME Lipocahn signamre CONSENSUS [DENG]-x-[DENQGSTARK]-x(0,2)-[DENQARK]-[LIVFY]-{CP}-G-{C}-W-[FYWLRH]-x- CONSENSUS [LIVMTA] NAME Cytosolic fatty-acid binding proteins signamre CONSENSUS [GSAIVK]-x-[FYW]-x-[LIVMF]-x(4)-[NHG]-[FY]-[DE]-x-[LIVMFY]-[LIVM]-x(2)- CONSENSUS [LIVMAKR] NAME Acyl-CoA-binding protein signamre CONSENSUS P-[STA]-x-[DEN]-x-[LIVMF]-x(2)-[LIVMFY]-Y-[GSTA]-x-[FY]-K-Q-[STA](2)-x-G NAME LBP / BPI / CETP family signamre CONSENSUS [PA]-[GA]-[LΓVMC]-X(2)-R-[IV]-[ST]-X(3)-L-X(5)-[EQ]-X(4)-[LIVM]-[EQK]- CONSENSUS x(8)-P NAME Phosphatidylethanolamiπe-binding protein family signamre CONSENSUS [FY]-x-[LIVMF](3)-x-[DC]-P-D-x-P-[SN]-x( 10)-H NAME Plant lipid transfer proteins signamre CONSENSUS [LIVM]-[PA]-x(2)-C-x-[LIVM]-x-[LIVM]-x-[LIVMFY]-x-[LIVM]-[ST]-x(3)- CONSENSUS [DN]-C-x(2)-[LIVM] NAME Uteroglobin family signamre 1 CONSENSUS [GA]-x(3)-I C-P-x-[LIVMF]-x(3)-[LIVM]-[DE]-x-[LIVMF](2) NAME Uteroglobin family signamre 2 CONSENSUS [DEQ]-x(4)-[SN]-x(5)-[DEQ]-x-I-x(2) S-[PSE]-[LS]-C NAME Mitochondrial energy transfer proteins signamre CONSENSUS P-x-[DE]-x-[LIVAT]-[RK]-x-[LRH]-[LIVMFY]-[QMAIGV] NAME Sugar transport proteins signamre 1 CONSENSUS [LrVMSTAG]-[LIVMFSAG]-x(2)-[LIVMSA]-[DE]-x-[LIVMFYWA]-G-R-[RK]-x(4,6)- CONSENSUS [GSTA] NAME Sugar transport proteins signamre 2 CONSENSUS [LIVMF]-x-G-[LIVMFA]-x(2)-G-x(8)-[LIFY]-x(2)-[EQ]-x(6)-[RK] NAME LacY family proton/sugar symporters signamre 1 CONSENSUS G-[LIVM](2)-x-D-[RK]-L-G-L-[RK](2)-x-[LIVM](2)-W NAME LacY family proton/sugar symporters signamre 2 CONSENSUS P-x-[LIVMF](2)-N-R-[LIVM]-G-x-K-N-[STA]-[LIVM](3) NAME PTR2 family proton/oligopeptide symporters signamre 1 CONSENSUS [GA]-[GAS]-[LIVMFYWA]-[LIVM]-[GAS]-D-X-[LIVMFYWT]-[LΓVMFYW]-G-X(3)-[TAV]- CONSENSUS [IV]-X(3)-[GSTAV]-X-[LIVMF]-X(3)-[GA] NAME PTR2 family proton ohgopepnde symporters signamre 2 CONSENSUS [FYT]-X(2)-[LMFY]-[FYV]-[LIVMFYWA]-X-[IVG]-N-[LIVMAG]-G-[GSA]-[LIMF] NAME Amilonde-sensitive sodium channels signature CONSENSUS Y-x(2)-[EQTF]-x-C-x(2)-[GSTDNL]-C-x-[QT]-x(2)-[LIVMT]-[LIVMS]-x(2)-C-x-C NAME Sodium alamne symporter family signamre CONSENSUS G-G-X-[GA](2)-[LΓVM]-F-W-M-W-[LΓVM]-X-[STAV]-[LIVMFA](2)-G NAME Sodium dicarboxylate symporter family signamre 1 CONSENSUS P-x(0,l)-G-[DE]-x-[LIVMF](2)-x-[LIVM](2)-[KREQ]-[LIVM](3)-x-P NAME Sodium dicarboxylate symporter family signamre 2 CONSENSUS P-x-G-x-[STA]-x-[NT]-[LIVMC]-D-G-[STAN]-x-[LIVM]-[FY]-x(2)-[LIVM]-x(2)- CONSENSUS [LIVM]-[FY]-[LI]-[SA]-Q NAME Sodium galactoside symporter family signamre CONSENSUS D-x(3)-G-x(3)-[DN]-x(6,8)-G-[KH]-F-[KR]-P-[FYW]-[LIVM](2)-x-[GSTA](2) NAME Sodium neurotransmitter symporter family signamre 1 CONSENSUS W-R-F-[GP]-Y-x(4)-N-G-G-G-x-[FY] NAME Sodium neurotransmitter symporter family signamre 2 CONSENSUS Y-[LIVMFY]-x(2)-[SC]-[LIVMFY]-[STQ]-x(2)-L-P-W-x(2)-C-x(4)-N-[GST] NAME Sodium solute symporter family signamre 1 CONSENSUS [GS]-x(2)-[LIY]-x(3)-[LIVMFYWSTAG](10)-[LIY]-[TAV]-x(2)-G-G-[LMF]-x- CONSENSUS [SAP] NAME Sodium solute symporter family signamre 2 CONSENSUS [GAST]-[LrVM]-x(3)-[KR]-x(4)-G-A-x(2)-[GAS]-[LIVMGS]-[LIVMW]-[LIVMGAT]-G- CONSENSUS x-[LIVMG] NAME Sodium sulfate symporter family signamre CONSENSUS [STACP]-S-x(2)-F-x(2)-P-[LIVM]-[GSA]-x(3)-N-x-[LIVM]-V glpT family of transporters signamre CONSENSUS R-G-x(5)-W-N-x(2)-H-N-x-G-G NAME Ammonium transporters signamre CONSENSUS D-[FYWS]-A-G-[GSC]-x(2)-[IV]-x(3)-[SAG](2)-x(2)-[SAG]-[LIVMF]-x(3)- CONSENSUS [LIVMFYWA](2)-x-[GK]-x-R NAME BCCT family of transporters signamre CONSENSUS [GSDN]-W-T-[LIVM]-x-[FY]-W-x-W- NAME Flagellar motor protein motA family signamre CONSENSUS A-[LMF]-x-[GAT]-T-[LIVF]-x-G-x-[LIVMF]-x(7)-P NAME Formate and mtnte transporters signamre 1 CONSENSUS [LIVMA]-[LIVMY]-x-G-[GSTA]-[DES]-L-[FI]-[TN]-[GS] NAME Formate and mtnte transporters signamre 2 CONSENSUS [GA]-x(2)-[CA]-N [LIVMFYW](2)-V-C-[LV]-A NAME Prokaryotic sulfate-binding proteins signamre 1 CONSENSUS K-x-[NQEK]-[GT]-G-[DQ]-x-[LIVM]-x(3)-Q-S NAME Prokaryonc sulfate-binding proteins signamre 2 CONSENSUS N-P-K-[ST]-S-G-x-A-R NAME Sulfate transporters signamre CONSENSUS P-x-Y-[GS]-L-Y-[STAG](2)-x(4)-[LIVMFY](3)-x(3)-[GSTA](2)-S-[KR] NAME Amino acid permeases signamre CONSENSUS [STAGC]-G-[PAG]-x(2,3)-[LIVMFYWA](2)-x-[LiVMFYW]-x-[LrVMFWSTAGC](2)- CONSENSUS [STAGC]-x(3)-[LIVMFYW]-x-[LIVMST]-x(3)-[LIMCTA]-[GA]-E-x(5)-[PSAL] NAME Aromatic ammo acids permeases signamre CONSENSUS I-G-[GA]-G-M-[LF]-[SA]-x-P-x(3)-[SA]-G-x(2)-F NAME Xanthine/uracil permeases family signamre CONSENSUS [LΓVM]-P-X-[PASIF]-V-ΓLIVM]-G-G-X(4)-[LIVM]-[FY]-[GSA]-X-[LIVM]-X(3)-G NAME Amon exchangers family signamre 1 CONSENSUS F-G-G-[LIVM](2)-[KR]-D-[LIVM]-[RK]-R-R-Y NAME Amon exchangers family signamre 2 CONSENSUS [FTJ-L-I-S-L-I-F-I-Y-E-T-F-x-K-L NAME MIP family signamre CONSENSUS [HNQA]-x-N-P-[STA]-[LIVMF]-[ST]-[LIVMF]-[GSTAFY] NAME General diffusion Gram-negative poπns signamre CONSENSUS: [LIVMFY]-x(2)-G-x(2)-Y-x-F-x-K-x(2)-[SN]-[STAV]-[LIVMFYW]-V NAME: OmpA-hke domain. CONSENSUS [LIVMA]-x-[GT]-x-[TA]-[DA]-x(2)-[DG]-[GSTP]-x(2)-[LFYDE]-[NQS]-x(2)- CONSENSUS. [LI]-[SG]-[QE]-[KRQE]-R-A-x(2)-[LV]-x(3)-[LIVMF]-x(4,5)-[LIVM]-x(4)- CONSENSUS- [LIVM]-x(3)-[SG]-x-G NAME: Eukaryotic mitochondπal poπn signamre. CONSENSUS: [YH]-x(2)-D-[SPA]-x-[STA]-x(3)-[TAG]-[KR]-[LIVMF]-[DNSTA]-[DNS]-x(4)- CONSENSUS: [GSTAN]-[LIVMA]-x-[LIVMY] NAME Insulin-like growth factor binding proteins signamre CONSENSUS. G-C-[GS]-C-C-x(2)-C-A-x(6)-C. NAME: GPRl/FUN34/yaaH family signature CONSENSUS: N-P-[AV]-P-[LF]-G-L-x-[GSA]-F. NAME: GNS1/SUR4 family signamre. CONSENSUS. L-x-F-L-H-x-Y-H-H. NAME: 43 Kd postsynaptic protein signamre CONSENSUS- G-Q-D-Q-T-K-Q-Q-I. NAME Actins signamre 1 CONSENSUS: [FY]-[LIV]-G-[DE]-E-A-Q-x-[RKQ](2)-G. NAME Actins signamre 2 CONSENSUS: W-[IV]-[STA]-[RK]-x-[DE]-Y-[DNE]-[DE] . NAME Actins and actm-related proterns signamre CONSENSUS: [LM]-[LIVM]-T-E-[GAPQ]-x-[LIVMFYWHQ]-N-[PSTAQ]-x(2)-N-[KR]. NAME Annexins repeated domain signamre. CONSENSUS [TG]-[STV]-x(8)-[LIVMF]-x(2)-R-x(3)-[DEQNH]-x(7)-[IFY]-x(7)-[LIVMF]- CONSENSUS x(3)-[LIVMF]-x(l l)-[LIVMFA]-x(2)-[LIVMF]. NAME. Caveolins signamre. CONSENSUS- F-E-D-V-I-A-E-P NAME: Clathrm light chain signamre 1. CONSENSUS: F-L-A-Q-Q-E-S NAME- Clathrm light chain signamre 2. CONSENSUS: [KR]-D-x-S-[KR]-[LIVM]-[KR]-x-[LIVM](3)-x-L-K NAME: Clusteπn signamre 1 CONSENSUS: C-K-P-C-L-K-x-T-C NAME: Clusteπn signamre 2. CONSENSUS: C-L-[RK]-M-[RK]-x-[EQ]-C-[ED]-K-C. NAME: Connexins signamre 1. CONSENSUS. C-[DN]-T-x-Q-P-G-C-x(2)-V-C-Y-D NAME: Connexins signamre 2. CONSENSUS: C-x(3,4)-P-C-x(3)-[LIVM]-[DEN]-C-[FY]-[LIVM]-[SA]-[KR]-P. NAME. Crystallins beta and gamma 'Greek key' motif signamre. CONSENSUS: [LrVMFYWA]-x-{DEHRKSTP}-[FY]-[DEQHKY]-x(3)-[FY]-x-G-x(4)-[LrVMFCST]. NAME: Dynamin family signamre CONSENSUS: L-P-[RK]-G-[STN]-[GN]-[LIVM]-V-T-R. NAME: Dynein light chain type 1 signamre. CONSENSUS. H-x-I-x-G-[KR]-x-F-[GA]-S-x-V-[ST]-[HY]-E. NAME: FtsZ protein signamre 1. CONSENSUS: N-[ST]-D-x-Q-x-L-x(16,18)-G-x-G-[ATV]-G-[GSAN]-x-P-x(2)-G. NAME: FtsZ protein signamre 2. CONSENSUS [DNHKR]-[LΓVMF]-X-[LΓVMF](2)-[VSTAC]-[STAC]-G-X-G-[GK]-G-T-G-[ST]-G- CONSENSUS: [GSAR]-[STA]-P-[LIVMFT]-[LIVMF]-[SGAV] NAME Fungal hydrophobins signamre CONSENSUS [GN]-[DNQPSA]-X-C-[GSTANK]-[GSTADNQ]-[STNQI]-[PTΓV]-X-C-C-[DENQKPST] NAME Intermediate filaments signamre CONSENSUS [IV]-x-[TACI]-Y-[RKH]-x-[LM]-L-[DE] NAME Involucrin signamre CONSENSUS < M-S-[QH]-Q-x-T-[LV]-P-V-T-[LV] NAME Kinesin motor domain signamre CONSENSUS [GSA]-[KRHPSTQVM]-[LIVMF]-x-[LIVMF]-[IVC]-D-L-[AH]-G-[SAN]-E NAME Kinesin motor domain profile NAME Kinesin light chain repeat CONSENSUS [DEQR]-A-L-x(3)-[GEQ]-x(3)-G-x-[DNS]-x-P-x-V-A-x(3)-N-x-L-[AS]- CONSENSUS x(5)-[QR]-x-[KR]-[FY]-x(2)-[AV]-x(4)-[HKNQ] NAME Myelin basic protein signamre CONSENSUS V-V-H-F-F-K-N NAME Myelin PO protein signamre CONSENSUS S-[KR]-S-x-K-[AG]-x-[SA]-E-K-K-[STA]-K NAME Myelin proteo pid protein signamre 1 CONSENSUS G-[MV]-A-L-F-C-G-C-G-H NAME Myelin proteohpid protein signamre 2 CONSENSUS C-x-[ST]-x-[DE]-x(3)-[ST]-[FY]-x-L-[FY]-I-x(4)-G-A NAME Neuromoduhn (GAP-43) signamre 1 CONSENSUS <M-L-C-C-[LIVM]-R-R NAME Neuromodulin (GAP-43) signamre 2 CONSENSUS S-F-R-G-H-I-x-R-K-K-[LIVM] NAME Osteopontin signature CONSENSUS [KQ]-x-[TA]-x(2)-[GA]-S-S-E-E-K NAME Penpheπn / rom-1 signamre CONSENSUS D-[GS]-V-P-F-[ST]-C-C-N-P-x-S-P-R-P-C NAME Profilin signamre CONSENSUS < x(0,l) [STA]-x(0,l)-W-[DENQH]-x-[YI]-x-[DEQ] NAME Surfactant associated polypeptide SP-C palmitoylation sites CONSENSUS I-P-C-C-P-V NAME Synapsins signamre 1 CONSENSUS L-R-R-R-L-S-D-S NAME Synapsins signamre 2 CONSENSUS G-H-A-H-S-G-M-G-K-V-K NAME Synaptobrevm signamre CONSENSUS N-[LIVM]-[DENS]-[KL]-V-x-[DEQ]-R-x(2)-[KR]-[LIVM]-[STDE]-x-[LIVM]-x-[DE]- CONSENSUS [KR]-[TA]-[DE] NAME Synaptophysin / synaptoporm signamre CONSENSUS L-S-V-[DE]-C-x-N-K-T NAME Tropomyosins signature CONSENSUS L-K-E-A-E-x-R-A-E NAME Tubulin subunits alpha, beta, and gamma signamre CONSENSUS [SAG]-G-G-T-G-[SA]-G NAME Tubulin-beta mRNA autoregulation signal CONSENSUS <M-R-[DE]-[IL] NAME Tau and MAP proteins tubulin-binding domain signamre CONSENSUS G-S-x(2)-N-x(2)-H-x-[PA]-[AG]-G(2) NAME Neuraxin and MAP1B proteins repeated region signamre CONSENSUS [STAGDN]-Y-x-Y-E-x(2)-[DE]-[KR]-[STAGCI] NAME F-actin capping protein alpha subunit signamre 1 CONSENSUS V-H-[FY](2)-E-D-G-N-V NAME F-actin capping protein alpha subunit signature 2 CONSENSUS F-K-[AE]-L-R-R-x-L-P NAME F-actin capping protein beta subumt signature CONSENSUS C-D-Y-N-R-D NAME Vincuhn family talin-binding region signamre CONSENSUS [KR]-x-[LIVMF]-x(3)-[LIVMA]-x(2)-[LIVM]-x(6)-R-Q-Q-E-L NAME Vincuhn repeated domain signamre CONSENSUS [LIVM]-x-[QA]-A-x(2)-W-[IL]-x-[DN]-P NAME Amyloidogenic glycoprotein extracellular domain signamre CONSENSUS G-[VT]-E-[FY]-V-C-C-P NAME Amyloidogenic glycoprotein intracellular domain signamre CONSENSUS G-Y-E-N-P-T-Y-[KR] NAME Cadheπns extracellular repeated domain signamre CONSENSUS [LIV]-x-[LrV]-x-D-x-N-D-[NH]-x-P NAME Insect cuticle proteins signamre CONSENSUS G-x(7)-[DEN]-G-x(6)-Y-x-A-[DNG]-x(2,3)-G-[FY]-x-[AP] NAME Gas vesicles protein GVPa signamre 1 CONSENSUS [LIVM]-x-[DE]-[LIVMFYT]-[LIVM]-[DE]-x-[LIVM](2)-[DKR](2)-G-x-[LIVM](2) NAME Gas vesicles protein GVPa signamre 2 CONSENSUS R-[LIVA](3)-A-[GS]-[LΓVMFY]-X-T-X(3)-Y-[AG] NAME Gas vesicles protein GVPc repeated domam signamre CONSENSUS F-L-x(2)-T-x(3)-R-x(3)-A-x(2)-Q-x(3)-L-x(2)-F NAME Bacterial microcompartiments proteins signamre CONSENSUS D-x(0, 1 )-M-x-K-[SAG](2)-x-[IV]-x-[LIVM]-[LrVMA]-[GCS]-x(4)-[GD]-[SGPD]- CONSENSUS [GA] NAME Flagella basal body rod proteins signamre CONSENSUS [GTARYQ]-x(9)-[LIVMYSTA](2)-[GSTA]-[STADEN]-N-[LIVM]-[SAN]-N-x-[SADNFR]- CONSENSUS [STV] NAME Flagella transport protein fliP family signamre 1 CONSENSUS [PA]-A-[FY]-x-[LIVT]-[STH]-[EQ]-[LI]-x(2)-[GA]-F-[KREQ]-[IM]-G-[LIF] NAME Flagella transport protein fliP family signamre 2 CONSENSUS P-[LIVMF]-K-[LIVMF](5)-x-[LIVMA]-[DNGS]-G-W NAME Plant viruses icosahedral capsid proteins 'S' region signamre CONSENSUS [FYW]-x-[PSTA]-x(7)-G-x-[LIVM]-x-[LIVM]-x-[FYWI]-x(2)-D-x(5)-P NAME Potexviruses and carlaviruses coat protem signamre CONSENSUS [RK]-[FYW]-A-[GAP]-F-D-x-F-x(2)-[LV]-x(3)-[GAST](2) NAME Neurotransmitter-gated ion-channels signamre CONSENSUS C-x-[LIVMFQ]-x-[LIVMF]-x(2)-[FY]-P-x-D-x(3)-C NAME ATP P2X receptors signamre CONSENSUS G-G-x-[LIVM]-G-[LrVM]-x-rrV]-x-W-x-C-[DN]-L-D-x(5)-C-x-P-x-Y-x-F NAME G-protein coupled receptors signamre CONSENSUS [GSTALIVMFYWC]-[GSTANCPDE]-{EDPKRH}-X(2)-ΓLΓVMNQGA]-X(2)-[LIVMFT]- CONSENSUS [GSTANC]-[LΓVMFYWSTAC]-[DENH]-R-[FYWCSH]-X(2)-[LIVM] NAME G-protem coupled receptors family 2 signamre 1 CONSENSUS C-X(3)-[FYWLIV]-D-X(3,4)-C-[FW]-X(2)-[STAGV]-X(8,9)-C-[PF] NAME G-protein coupled receptors family 2 signamre 2 CONSENSUS Q-G-[LMFCA]-[LrVMFT]-[LrV]-x-[LrVFST]-[LIF]-ryFYH]-C-[LFY]-x-N-x(2)-V NAME G-protein coupled receptors family 3 signamre 1 CONSENSUS [LV]-x-N-[LIVM](2)-x-L-F-x-I-[PA]-Q-[LIVM]-[STA]-x-[STA](3)-[STAN] NAME G-protein coupled receptors family 3 signamre 2 CONSENSUS C-C-[FYW]-x-C-x(2)-C-x(4)-[FYW]-x(2,4)-[DN]-x(2)-[STAH]-C-x(2)-C NAME G-protein coupled receptors family 3 signamre 3 CONSENSUS F-N-E-[STA]-K-x-I-[STAG]-F-[ST]-M NAME Visual pigments (opsins) retinal binding site CONSENSUS [LIVMWAC]-[PGAC]-x(3)-[SAC]-K-[STALIMR]-[GSACPNV]-[STACP]-x(2)-[DENF]- CONSENSUS [AP]-x(2)-[IY] NAME Bacterial rhodopsins signamre 1 CONSENSUS R-Y-x-[DT]-W-x-[LIVMF]-[ST]-T-P-[LrVM](3) NAME Bacterial rhodopsins retinal binding site CONSENSUS [FYIV]-x-[FYVG]-[LIVM]-D-[LIVMF]-x-[STA]-K-x(2)-[FY] NAME Receptor tyrosine kinase class II signamre CONSENSUS [DN]-[LIV]-Y-x(3)-Y-Y-R NAME Receptor tyrosine kinase class III signamre CONSENSUS G-x-H-x-N-[LIVM]-V-N-L-L-G-A-C-T NAME Receptor tyrosine kinase class V signamre 1 CONSENSUS F-X-[DN]-X-[GAW]-[GA]-C-[LΓVM]-[SA]-[LIVM](2)-[SA]-[LV]-[KRHQ]-[LIVA]- CONSENSUS x(3)-[KR]-C-[PSAW] NAME Receptor tyrosine kinase class V signamre 2 CONSENSUS C-x(2)-[DE]-G-[DEQ]-W-x(2,3)-[PAQ]-[LIVMT]-[GT]-x-C-x-C-x(2)-G-[HFY]- CONSENSUS [EQ] NAME Growth factor and cytokines receptors family signature 1 CONSENSUS C-[LVFYR]-x(7,8)-[STIVDN]-C-x-W NAME Growth factor and cytokines receptors family signamre 2 CONSENSUS [STGL]-x-W [SG]-x-W-S NAME TNFR/NGFR family cysteine-rich region signamre CONSENSUS C-x(4,6)-[FYH]-x(5, 10)-C-x(0,2)-C-x(2,3) C-x(7, 1 l)-C-x(4,6)-[DNEQSKP]- CONSENSUS x(2)-C NAME TNFR/NGFR family cysteine-πch region domain NAME Integnns alpha chain signamre CONSENSUS [FYWS]-[RK]-x-G-F-F-x-R NAME Integnns beta chain cysteine-rich domain signamre CONSENSUS C-x-[GNQ]-x(l ,3)-G-x-C-x-C-x(2)-C-x-C NAME Natπuretic peptides receptors signamre CONSENSUS G-P-x-C-x-Y-x-A-A-x-V-x-R-x(3)-H-W NAME Photosynthetic reaction center proteins signamre CONSENSUS [NH]-x(4)-P-x-H-x(2)-[SAG]-x(ll)-[SAGC]-x-H-[SAG](2) NAME Antenna complexes alpha subunits signamre CONSENSUS [LIVFAG]-x-[GASV]-[LIVFA]-x-[IV]-H-x(3)-[LrVM]-[GSTAE]-[STANH]-x(l,3)- CONSENSUS [STN]-W-[LIVMFYW] NAME Antenna complexes beta subunits signamre CONSENSUS [EQ]-x(4)-H-x(5)-[GSTA]-x(3)-[FY]-x(3)-[AG]-x(2)-[AV]-H-x(7)-P NAME Photosystem I psaA and psaB proteins signamre CONSENSUS C-D-G-P-G-R-G-G-T-C NAME Photosystem I psaG and psaK proteins signamre CONSENSUS G-F-x-[LIVM]-x-[DEA]-x(2)-[GA]-x-[GTA]-[SA]-x-G-H-x-[LIVM]-[GA] NAME Phytochrome chromophore attachment site signamre CONSENSUS [RGS]-[GSA]-[PV]-H-x-C-H-x(2)-Y NAME Phytochrome chromophore attachment site domain profile NAME Speract receptor repeated domain signamre CONSENSUS G-x(5)-G-x(2)-E-x(6)-W-G-x(2)-C-x(3)-[FYW]-x(8)-C-x(3)-G NAME TonB-dependent receptor proteins signamre 1 CONSENSUS < x(10, 115)-[DENF]-[ST]-[LIVMF]-[LIVSTEQ]-V-x-[AGP]-[STANEQPK] NAME TonB-dependent receptor proteins signamre 2 CONSENSUS [LYGSTANE]-x(3)-[GSTAENQ]-x-[PGE]-R-x-[LIVFYWA]-x-[LrVMFTA]-[STAGNQ]- CONSENSUS [LIVMFYGTA]-x-[LIVMFYWGTADQ]-x-F > NAME Transmembrane 4 family signamre CONSENSUS G-x(3)-[LIVMF]-x(2)-[GSA]-[LIVMF](2)-G-C-x-[GA]-[STA]-x(2)-[EG]-x(2)- CONSENSUS [CWN]-[LIVM](2) NAME Bacterial chemotaxis sensory transducers signamre CONSENSUS R-T-E-[EQ]-Q-x(2)-[SA]-[LIVM]-x-[EQ]-T-A-A-S-M-E-Q-L-T-A-T-V NAME ER lumen protein retaining receptor signamre 1 CONSENSUS G-I-S-x-[KR]-x-Q-x-L-[FY]-x-[LIV](2)-F-x(2)-R-Y NAME ER lumen protein retaining receptor signamre 2 CONSENSUS L-E-[SA]-V-A-I-[LM]-P-Q-L NAME Ephπns signamre CONSENSUS [KRQ]-[LF]-[CST]-x-K-[IF]-Q-x-[FY]-[ST]-[PA]-x(3)-G-x-E-F-x(5)-[FY](2)- CONSENSUS x(2)-[SA] NAME Granulms signamre CONSENSUS C-x-D-x(2)-H-C-C-P-x(4)-C NAME HBGF/FGF family signamre CONSENSUS G-x-L-x-[STAGP]-x(6,7)-[DE]-C-x-[FM]-x-E-x(6)-Y NAME PTN/MK hepaπn-binding protein family signamre 1 CONSENSUS S-[DE]-C-x-[DE]-W-x-W-x(2)-C-x-P-x-[SN]-x-D-C-G-[LIVMA]-G-x-R-E-G NAME PTN/MK hepaπn-binding protein family signature 2 CONSENSUS C-[KR]-[LIVM]-P-C-N- -K-K-x-F-G-A-[DE]-C-K-Y-x-F-[EQ]-x-W-G-x-C NAME Nerve growth factor family signamre CONSENSUS G-C-[KR]-G-[LIV]-[DE]-x(3)-[YW]-x-S-x-C NAME Platelet-deπved growth factor (PDGF) family signamre CONSENSUS P-[PS]-C-V-x(3)-R-C-[GSTA]-G-C-C NAME Small cytokines (intercnne/chemokine) C-x-C subfamily signamre CONSENSUS C-x-C-[LIVM]-x(5,6)-[LIVMFY]-x(2)-[RKSEQ]-x-[LIVM]-x(2)-[LIVM]-x(5)- CONSENSUS [SAG]-x(2)-C-x(3)-[EQ]-[LIVM](2)-x(9, 10)-C-L-[DN] NAME Small cytokines (intercnne/chemokine) C-C subfamily signamre CONSENSUS C-C-[LIFYT]-x(5,6)-[LI]-x(4)-[LIVMF]-x(2)-[FYW]-x(6,8)-C-x(3,4)-[SAG]- CONSENSUS [LIVM](2)-[FL]-x(8)-C-[STA] NAME TGF-beta family signamre CONSENSUS [LrVM]-x(2)-P-x(2)-[FY]-x(4)-C-x-G-x-C NAME TNF family signamre CONSENSUS [LV]-x-[LIVM]-x(3)-G-[LIVMF]-Y-[LIVMFY](2)-x(2)-[QEKHL]-[LIVMGT]-x- CONSENSUS [LIVMFY] NAME TNF family profile NAME Wnt-1 family signamre CONSENSUS C-K-C-H-G-[LIVMT]-S-G-x-C NAME Interferon alpha, beta and delta family signamre CONSENSUS [FYH]-[FY]-x-[GNRC]-[LIVM]-x(2)-[FY]-L-x(7)-[CY]-A-W NAME Granulocyte-macrophage colony-stimulanng factor signamre CONSENSUS C-P-[LP]-T-x-E-[ST]-x-C NAME Interleukin- 1 signamre CONSENSUS [FC]-x-S-[ASLV]-x(2)-P-x(2)-[FYLIV]-[LI]-[SCA]-T-x(7)-[LIVM] NAME Interleukιn-2 signamre CONSENSUS T-E-[LF]-x(2)-L-x-C-L-x(2)-E-L NAME Interleukins -4 and -13 signamre CONSENSUS L-x-E-[LIVM](2)-x(4,5)-[LIVM]-[TL]-x(5,7)-C-x(4)-[IVA]-x-[DNS]-[LIVMA] NAME Interleukιn-6 / G-CSF / MGF signamre CONSENSUS C-x(9)-C-x(6)-G-L-x(2)-[FY]-x(3)-L NAME Interleukm-7 and -9 signamre CONSENSUS N-x-[LAP]-[SCT]-F-L-K-x-L-L NAME Interleukin- 10 family signamre CONSENSUS [GS]-C-x(2)-[LV]-x(2)-[LIVM](2)-x-F-Y-L-x(2)-V NAME LIF / OSM family signamre CONSENSUS [PST]-x(4)-F-[NQ]-x-K-x(3)-C-x-[LF]-L-x(2)-Y-[HK] NAME Macrophage migration inhibitory factor family signamre CONSENSUS [DE]-P-C-A-x(3)-[LIVM]-x-S-I-G-x-[LIVM]-G NAME Adipokinetic hormone family signamre CONSENSUS Q-[LV]-[NT]-[FY]-[ST]-x(2)-W NAME Bombesin-like peptides family signamre CONSENSUS W-A-x-G-[SH]-[LF]-M NAME Calcitomn / CGRP / IAPP family signamre CONSENSUS C-[SAGDN]-[STN]-x(0, 1 )-[SA]-T-C-[VMA]-x(3)-[LYF]-x(3)-[LYF] NAME Corticotropin-releasing factor family signamre CONSENSUS [PQ]-x-[LIVM]-S-[LIVM]-x(2)-[PST]-[LIVMF]-x-[LIVM]-L-R-x(2)-[LIVM] NAME Crustacean CHH/MIH/GIH neurohoπnones family signamre CONSENSUS C-[DENK]-D-C-x N-[LIV]-[FY]-R-x(7)-C-[KR]-x(2)-C NAME Erythropoietin / thrombopoeitin signamre CONSENSUS P-x(4)-C-D-x-R-[LIVM](2)-x-[KR]-x(14)-C NAME Granins signamre 1 CONSENSUS [DE]-[SN]-L-[SAN]-x(2)-[DE]-x-E-L NAME Gramns signamre 2 CONSENSUS C-[LIVM](2)-E-[LIVM](2)-S-[DN]-[STA]-L-x-K-x-S-x(3)-[LIVM]-[STA]-x-E-C NAME Galamn signamre CONSENSUS G-W-T-L-N-S-A-G-Y-L-L-G-P-H NAME Gastnn / cholecystokimn family signamre CONSENSUS Y-x(0,l)-[GD]-[WH]-M-[DR]-F NAME Glucagon / GIP / secretin / VIP family signamre CONSENSUS [YH]-[STAΓVGD]-[DEQ]-[AGF]-[LIVMSTE]-[FYLR]-X-[DENSTAK]-[DENSTA]- CONSENSUS [LIVMFYG]-X(9)-[KREQL]-[KRDENQL]-[LVFYWG]-[LIVQ] NAME Glycoprotein hormones alpha chain signamre 1 CONSENSUS C-x-G-C-C-[FY]-S-R-A-[FY]-P-T-P NAME Glycoprotem hormones alpha chain signamre 2 CONSENSUS N-H-T-x-C-x-C-x-T-C-x(2)-H-K NAME Glycoprotem hormones beta chain signamre 1 CONSENSUS C-[STAGM]-G-[HFYL]-C-x-[ST] NAME Glycoprotein hormones beta chain signamre 2 CONSENSUS [PA]-V-A-x(2)-C-x-C-x(2)-C-x(4)-[STD]-[DEY]-C-x(6,8)-[PGSTAVM]-x(2)-C NAME Gonadotropin-releasing hormones signamre CONSENSUS Q-H-[FYW]-S-x(4)-P-G NAME Insulin family signamre CONSENSUS C-C-{P}-x(2)-C-[STDNEKPI]-x(3)-[LIVMFS]-x(3)-C NAME Natπuretic peptides signamre. CONSENSUS C-F-G-x(3)-D-R-I-x(3)-S-x(2)-G-C NAME Neurohypophysial hormones signamre. CONSENSUS: C-[LIFY](2)-x-N-[CS]-P-x-G. NAME Neuromedin U signamre CONSENSUS: F-[LIVMF]-F-R-P-R-N NAME: Endogenous opioids neuropeptides precursors signamre. CONSENSUS: C-x(3)-C-x(2)-C-x(2)-[KRH]-x(6,7)-[LIF]-[DN]-x(3)-C-x-[LIVM]-[EQ]-C- CONSENSUS: [EQ]-x(8)-W-x(2)-C NAME: Pancreatic hormone family signamre CONSENSUS: [FY]-x(3)-[LIVM]-x(2)-Y-x(3)-[LIVMFY]-x-R-x-R-[YF] NAME Parathyroid hormone family signamre. CONSENSUS: V-S-E-x-Q-x(2)-H-x(2)-G. NAME: Pyrokinins signamre. CONSENSUS F-[GSTV]-P-R-L-[G>] NAME: Somatotropin, prolactin and related hormones signamre 1. CONSENSUS: C-x-[ST]-x(2)-[LIVMFY]-x-[LIVMSTA]-P-x(5)-[TALIV]-x(7)-[LIVMFY]-x(6)- CONSENSUS: [LIVMFY]-x(2)-[STA]-W NAME. Somatotropin, prolactin and related hormones signamre 2 CONSENSUS: C-[LIVMFY]-x(2)-D-[LIVMFYSTA]-x(5)-[LIVMFY]-x(2)-[LrVMFYT]-x(2)-C NAME: Tachykinin family signamre. CONSENSUS- F-[IVFY]-G-[LM]-M-[G > ] NAME: Thymosin beta-4 family signamre CONSENSUS K-L-K-K-T-E-T-Q-E-K-N NAME Urotensin II signamre CONSENSUS C-F- -K-Y-C. NAME: Cecropin family signamre CONSENSUS. W-x(0,2)-[KDN]-x(2)-K-[KRE]-[LI]-E-[RKN]. NAME: Mammalian defensins signamre. CONSENSUS : C-x-C-x(3 ,5)-C-x(7)-G-x-C-x(9)-C-C . NAME: Arthropod defensins signature CONSENSUS C-x(2,3)-[HN]-C-x(3,4)-[GR]-x(2)-G-G-x-C-x(4,7)-C-x-C NAME- Cathelicidins signamre 1 CONSENSUS Y-x-[ED]-x-V-x-[RQ]-A-[LIVMA]-[DQG]-x-[LIVMFY]-N-[EQ] NAME: Cathelicidins signamre 2. CONSENSUS: F-x-[LIVM]-K-E-T-x-C-x(10)-C-x-F-[KR]-[KE] NAME: Endothelin family signamre. CONSENSUS: C-x-C-x(4)-D-x(2)-C-x(2)-[FY]-C. NAME: Plant thionins signamre CONSENSUS: C-C-x(5)-R-x(2)-[FY]-x(2)-C. NAME: Gamma-thionins family signamre CONSENSUS- [KR]-x-C-x(3)-[SV]-x(2)-[FYWH]-x-[GF]-x-C-x(5)-C-x(3)-C. NAME: Snake toxms signamre. CONSENSUS: G-C-x(l,3)-C-P-x(8,10)-C-C-x(2)-[PDEN]. NAME: Myotoxins signamre. CONSENSUS K-x-C-H-x-K-x(2)-H-C-x(2)-K-x(3)-C-x(8)-K-x(2)-C-x(2)-[RK]-x-K-C-C-K-K. NAME: Scorpion short toxins signamre. CONSENSUS C-x(3)-C-x(6,9)-[GAS]-K-C-[IMQT]-x(3)-C-x-C. NAME: Heat-stable enterotoxins signamre. CONSENSUS C-C-x(2)-C-C-x-P-A-C-x-G-C. NAME Aerolysin type toxins signamre CONSENSUS [KT]-x(2)-N-W-x(2)-T-[DN]-T NAME Shiga/πcin ribosomal inactivating toxins active site signature CONSENSUS [LIVMA]-x-[LIVMSTA](2)-x-E-[SAGV]-[STAL]-R-[FY]-[RKNQS]-x-[LIVM]-[EQS]- CONSENSUS x(2)-[LIVMF] NAME Channel forming cohcins signamre CONSENSUS T-x(2)-W-x-P-[LIVMFY](3)-x(2)-E NAME Hok gef family cell toxic proteins signamre CONSENSUS [LIVMA](4)-C-[LIVMFA]-T-[LIVMA](2)-x(4)-[LIVM]-x-[RG]-x(2)-L-[CY] NAME Staphylococcal enterotoxin Streptococcal pyrogemc exotoxin signamre 1 CONSENSUS Y-G-G-[LIV]-T-x(4)-N NAME Staphyloccocal enterotoxin/Streptococcal pyrogemc exotoxin signamre 2 CONSENSUS K-x(2)-[LrV]-x(4)-[LIV]-D-x(3)-R-x(2)-L-x(5)-[LIV]-Y NAME Thiol-activated cytolysins signamre CONSENSUS [RK]-E-C-T-G-L-x-W-E-W-W-[RK] NAME Membrane attack complex components / perform signamre CONSENSUS Y-x(6)-[FY]-G-T-H-[FY] NAME Pancreatic trypsin inhibitor (Kunitz) family signamre CONSENSUS F-x(3)-G-C-x(6)-[FY]-x(5)-C NAME Bowman-Birk serine protease inhibitors family signamre CONSENSUS C-x(5,6)-[DENQKRHSTA]-C-[PASTDH]-[PASTDK]-[ASTDV]-C-[NDKS]-[DEKRHSTA]-C NAME Kazal serine protease inhibitors family signamre CONSENSUS C-x(7)-C-x(6)-Y-x(3)-C-x(2, 3)-C NAME Soybean trypsin inhibitor (Kunitz) protease inhibitors family signamre CONSENSUS [LIVM]-x-D-x-[EDNTY]-[DG]-[RKHDENQ]-x-[LIVM]-x(5)-Y-x-[LIVM] NAME Serprns signamre CONSENSUS [LIVMFY]-x-[LIVMFYAC]-[DNQ]-[RKHQS]-[PST]-F-[LIVMFY]-[LIVMFYC]-x- CONSENSUS [LIVMFAH] NAME Potato inhibitor I family signamre CONSENSUS [FYW]-P-[EQH]-[LIV](2)-G-x(2)-[STAGV]-x(2)-A NAME Squash family of senne protease inhibitors signature CONSENSUS C-P-x(5)-C-x(2)-D-x-D-C-x(3)-C-x-C NAME Streptomyces subtihsin-type inhibitors signamre CONSENSUS C-x-P-x(2,3)-G-x-H-P-x(4)-A-C-[ATD]-x-L NAME Cysteme proteases inhibitors signature CONSENSUS [GSTEQKRV]-Q-[LIVT]-[VAF]-[SAGQ]-G-X-[LIVMNK]-X(2)-[LΓVMFY]-X-[LIVMFYA]- CONSENSUS [DENQKRHSIV] NAME Tissue inhibitors of metalloproteinases signamre CONSENSUS C-x-C-x-P-x-H-P-Q-x-A-F-C NAME Cereal trypsin/alpha-amylase inhibitors family signamre CONSENSUS C-x(4)-[SAGD]-x(4)-[SPAL]-[LF]-x(2)-C-[RH]-x-[LIVMFY](2)-x(3,4)-C NAME Alpha-2-macroglobulιn family thiolester region signature CONSENSUS [PG]-x-[GS]-C-[GA]-E-[EQ]-x-[LIVM] NAME Disintegnns signamre CONSENSUS C-x(2)-G-x-C-C-x-[NQRS]-C-x-[FM]-x(6)-C-[RK] NAME Lambdoid phages regulatory protein CIII signamre CONSENSUS E-S-x-L-x-R-x(2)-[KR]-x-L-x(4)-[KR](2)-x(2)-[DE]-x-L NAME Chaperonins cpn60 signamre CONSENSUS A-[AS]-x-[DEQ]-E-x(4)-G-G-[GA] NAME Chaperomns cpnlO signamre CONSENSUS [LIVMFY]-x-P-[ILT]-x-[DEN]-[KR]-[LιVMFA](3)-[KREQ]-x(8,9)-[SG]-x- CONSENSUS [LIVMFY](3) NAME Chaperonms TCP-1 signamre 1 CONSENSUS [RKEL]-[ST]-x-[LMFY]-G-P-x-[GSA]-x-x-K-[LIVMF](2) NAME Chaperomns TCP-1 signamre 2 CONSENSUS [LIVM]-[TS]-[NK]-D-[GA]-[AVNHK]-[TAV]-[LIVM](2)-x(2)-[LIVM]-x-[LIVM]-x- CONSENSUS [SNHHPQH] NAME Chaperomns TCP-1 signamre 3 CONSENSUS Q-[DEK]-x-x-[LIVMGTA]-[GA]-D-G-T NAME Heat shock hsp20 proteins family profile NAME Heat shock hsp70 proteins family signamre 1 CONSENSUS [IV]-D-L-G-T [ST]-x-[SC] NAME Heat shock hsp70 proteins family signamre 2 CONSENSUS [LIVMF]-[LIVMFY]-[DN]-[LIVMFS]-G-[GSH]-[GS]-[AST]-x(3)-[ST]-[LIVM]- CONSENSUS [LIVMFC] NAME Heat shock hsp70 proteins family signamre 3 CONSENSUS [LIVMY]-x-[LIVMF]-x-G-G-x-[ST]-x-[LIVM]-P-x-[LIVM]-x-[DEQKRSTA] NAME Heat shock hsp90 proteins family signamre CONSENSUS Y-x-[NQH]-K-[DE]-[IVA]-F-L-R-[ED] NAME Chaperomns clpA/B signamre 1 CONSENSUS D-[AIHSGA]-N-[LIVMF](2)-K-[PT]-x-L-x(2)-G NAME Chaperomns clpA/B signamre 2 CONSENSUS R-[LIVMFY]-D-x-S-E-[LIVMFY]-x-E-[KRQ]-x-[STA]-x-[STA]-[KR]-[LIVM]-x-G- CONSENSUS [STA] NAME Nt-dnaJ domain signamre CONSENSUS [FY]-x(2)-[LrVMA]-x(3)-[FYWHNT]-[DENQSA]-x-L-x-[DN]-x(3)-[KR]-x(2)-[FYI] NAME dnaJ domain profile NAME CXXCXGXG dnaJ domain signamre CONSENSUS C-[DEGSTHKR]-x-C-x-G-x-[GK]-[AGSDM]-x(2)-[GSNKR]-x(4,6)-C-x(2,3)-C-x-G-x-G NAME grpE protem signamre CONSENSUS [FL]-[DN]-[PHEA]-x(2)-[HM]-x-A-[LIVMTN]-x(16,20) G-[FY]-x(3)-[DEG]-x(2)- CONSENSUS [LIVM]-[RI]-x-[SA]-x-V-x-[IV] NAME Bacterial type II secretion system protein C signamre CONSENSUS P-x(6)-F-x(4)-L-x(3)-D-[LIVM]-A-[LIVM]-x-[LIVM]-N-x-[LIVM]-x-L NAME Bacterial type II secretion system protein D signamre CONSENSUS [GR]-[DEQKG]-[STVM]-[LIVMA](3)-[GA]-G-[LIVMFY]-x( 11)-[LIVM]-P- CONSENSUS [LIVMFYWGS]-[LIVMF]-[GSAE]-x-[LIVM]-P-[LIVMFYW](2)-x(2)-[LV]-F NAME Bacterial type II secretion system protein E signamre CONSENSUS [LIVM]-R-x(2)-P-D-x-[LIVM](3)-G-E-[LIVM]-R-D NAME Bacterial type II secretion system protein F signamre CONSENSUS [KRQ]-[LIVMA]-x(2)-[SAIV]-[LIVM]-x-[TY]-P-x(2)-[LrVM]-x(3)-[STAGV]-x(6)- CONSENSUS [LMY]-x(3)-[LIVMF](2)-P NAME Bacterial type II secretion system protein N signamre CONSENSUS G-T-L-W-x-G-x( 11 )-L-x(4)-W NAME Bacterial export FHIPEP family signature CONSENSUS R-[LΓVM]-[GSA]-E-V-[GSA]-A-R-F-[STV]-L-D-[GSA]-M-P-G-K-Q-M-[GSA]-I-D- CONSENSUS [GSA]-D NAME Protein secA signatures CONSENSUS [rV]-x-[IV]-[SA]-T-[NQ]-M-A-G-R-G-x-D-I-x-L NAME Protein secY signamre 1 CONSENSUS [GST]-[LIVMF](2)-x-[LIVM]-G-[LIVM]-x-P-[LIVMFY](2)-x-[AS]-[GSTQ]- CONSENSUS [LIVMFAT](3)-Q-[LrVMFA](2) NAME Protein secY signamre 2 CONSENSUS [LIVMFY ](2)-x-[DE]-x-[LIVMF]-[STN]-x(2)-G-[LIVMF]-[GST]-[NST]-G-x-[GST]- CONSENSUS [LIVMF](3) NAME Protein secE/sec61 -gamma signamre CONSENSUS [LIVMFY]-x(2)-[DENQGA]-x(4)-[LIVMTA]-x-[KRV]-x(2)-[KW]-P-x(3)-[SEQ]-x(7)- CONSENSUS [LIVTHLIVGAHLIVFGAST] NAME Gram-negative pill assembly chaperone signamre CONSENSUS [LIVMFY]-[APN]-x-[DNS]-[KREQ]-E-[STR]-[LIVMAR]-x-[FYWT]-x-[NC]-[LIVM]- CONSENSUS x(2)-[LIVM]-P-[PAS] NAME Fimbπal biogenesis outer membrane usher protein signamre CONSENSUS [VL]-[PASQ]-[PAS]-G-[PAD]-[FY]-x-[LI]-[DNQSTAP]-[DNH]-[LIVMFY] NAME SRP54-type proteins GTP-bindmg domain signamre CONSENSUS P-[LrVM]-x-[FYL]-[LIVMAT]-[GS]-x-[GS]-[EQ]-x(4)-[LIVMF] NAME Cytochrome c oxidase assembly factor COXlO/ctaB/cyoE signamre CONSENSUS [ED]-x-D-x(2)-M-x-R-T-x(2)-R-x(4)-G NAME Cyclin-dependent kinases regulatory subunits signamre 1 CONSENSUS Y-S-x-[KR]-Y-x-[DE](2)-x-[FY]-E-Y-R-H-V-x-[LV]-[PT]-[KRP] NAME Cyclin-dependent kinases regulatory subunits signamre 2 CONSENSUS H-x-P-E-x-H-[rV]-L-L-F-[KR] NAME Pentaxin family signamre CONSENSUS H-x-C-x-[ST]-W-x-[ST] NAME Immunoglobulins and major histocompatibility complex proteins signamre CONSENSUS [FY]-x-C-x-[VA]-x-H NAME Pπon protein signamre 1 CONSENSUS A-G-A-A-A-A-G-A-V-V-G-G-L-G-G-Y NAME Pπon protein signamre 2 CONSENSUS E-x-[ED]-x-K-[LIVM](2)-x-[KR]-[LIVM](2)-x-[QE]-M-C-x(2)-Q-Y NAME Cyclins signamre CONSENSUS R-x(2)-[LIVMSA]-x(2)-[FYWS]-[LIVM]-x(8)-[LIVMFC]-x(4)-[LIVMFYA]-x(2)- CONSENSUS [STAGC]-[LIVMFYQ]-x-[LIVMFYC]-[LIVMFY]-D-[RKH]-[LIVMFYW] NAME Proliferating cell nuclear antigen signamre 1 CONSENSUS [GA]-[LIVMF]-x-[LrVMA]-x-[SAV]-[LrVM]-D-x-[NSAE]-[HKR]-rVI]-x-[LY]- CONSENSUS [VGA]-x-[LIVM]-x-[LIVM]-x(4)-F NAME Proliferating cell nuclear antigen signamre 2 CONSENSUS [RKA]-C-[DE]-[RH]-x(3)-[LIVMF]-x(3)-[LIVM]-x-[SGAN]-[LrVMF]-x-K- CONSENSUS [LIVMF](2) NAME Acπn-depolymeπzing proteins signature CONSENSUS P-[DE]-x-[SA]-x-[LIVMT]-[KR]-x-[KR]-M-[LIVM]-[YA]-[STA](3)-x(3)-[LIVMF]- CONSENSUS [KR] NAME BCL2-hke apoptosis inhibitors (spans part of BH3, BH1 and BH2) NAME Apoptosis regulator, Bcl-2 family BH1 domain signamre CONSENSUS [LVME]-[FT]-x-[GSD]-[GL]-x( 1 ,2)-[NS]-[YW]-G-R-[LIV]-[LIVC]-[GAT]- CONSENSUS [LIVMF](2)-x-F-[GSAE]-[GSARY] NAME Apoptosis regulator, Bcl-2 family BH2 domain signamre CONSENSUS W-[LIM]-x(3)-[GR]-G-[WQ]-[DENSAV]-x-[FLGA]-[LIVFTC] NAME Apoptosis regulator, Bcl-2 family BH3 domam signamre CONSENSUS [LrVAT]-x(3)-L-[KARQ]-x-[IVAL]-G-D-[DESG]-[LIMFV]-[DENSHQ]-[LVSHROJ- CONSENSUS [NSR] NAME Apoptosis regulator, Bcl-2 family BH4 domain signamre CONSENSUS [DS]-[NT]-R-[AE]-[LI]-V-x-[KD]-[FY]-[LIV]-[GHS]-Y-K-L-[SR]-Q-[RK]-G- CONSENSUS [HY]-x-[CW] NAME Apoptosis regulator, Bcl-2 family BH4 domain profile NAME Arrestins signamre CONSENSUS [FY]-R-Y-G-X-[DE](2)-X-[DE]-[LΓVM](2)-G-[LIVM]-X-F-X-[RK]-[DEQ]-[LΓVM] NAME AAA-protein family signamre CONSENSUS [LΓVMT]-X-[LΓVMT]-[LIVMF]-X-[GATMC]-[ST]-[NS]-X(4)-[LIVM]-D-X-A-[LIFA]- CONSENSUS x-R NAME Ubiquitin domain signamre CONSENSUS K-X(2)-[LIVM]-X-[DESAK]-X(3)-[LIVM]-[PA]-X(3)-Q-X-[LIVM]-[LIVMC]- CONSENSUS [LIVMFY]-x-G-x(4)-[DE] NAME Ubiquitin domain profile NAME ADP-ribosylation factors family signamre CONSENSUS [HRQT]-x-[FYWI]-x-[LIVM]-x(4)-A-x(2)-G-x(2)-[LIVM]-x(2)-[GSA]-[LIVMF]-x- CONSENSUS [WKHLIVM] NAME GTP-binding nuclear protein ran signamre CONSENSUS D-T-A-G-Q-E-K-[LF]-G-G-L-R-[DE]-G-Y-Y NAME SARI family signamre CONSENSUS R-x-[LIVM]-E-V-F-M-C-S-[LIVM](2)-x-[KRQ]-x-G-Y-x-E-[AG]-[FI]-x-W-[LIVM]- CONSENSUS x-Q-Y NAME Band 7 protein family signamre CONSENSUS R-x(2)-[LIV]-[SAN]-x(6)-[LIV]-D-x(2)-T-x(2)-W-G-[LIV]-[KRH]-[LIV]-x- CONSENSUS [KR]-[LIV]-E-[LIV]-[KR] NAME Trp-Asp (WD) repeats signamre CONSENSUS [LIVMSTAC]-[LIVMFYWSTAGC]-[LIMSTAG]-[LIVMSTAGC]-x(2)-[DN]-x(2)- CONSENSUS [LIVMWSTAC]-x-[LIVMFSTAG]-W-[DEN]-[LIVMFSTAGCN] NAME G-protein gamma subunit profile NAME Ras GTPase-activating proteins signamre CONSENSUS [GSN]-X-[LΓVMF]-[FY]-[LIVMFY]-R-[LIVMFY](2)-[GACN]-P-[AV]-[LΓV](2)- CONSENSUS [SGAN]-P NAME Ras GTPase-activating proteins profile NAME Guanine-nucleotide dissociation stimulators CDC24 family signamre CONSENSUS L-x(2)-[LIVMFYW]-L-x(2)-P-[LIVM]-x(2)-[LIVM]-x-[KRS]-x(2)-L-x-[LIVM]-x- CONSENSUS [DEQ]-[LIVM]-x(3)-[ST] NAME Guamne-nucleotide dissociation stimulators CDC25 family signamre CONSENSUS [GAP]-[CT]-V-P-[FY]-x(4)-[LIVMFY]-x-[DN]-[LIVM] NAME MARCKS family signamre 1 CONSENSUS G-Q-E-N-G-H-V-[KR] NAME MARCKS family phosphorylation site domain CONSENSUS E-T-P-K(5)-x(0, 1 )-F-S-F-K-K-x-F-K-L-S-G-x-S-F-K-[KR]-[NS]-[KR]-K-E NAME Stathmin family signamre 1 CONSENSUS P-[KQ]-[KR](2)-[DE]-x-S-L-[EG]-E NAME Stathmin family signamre 2 CONSENSUS A-E-K-R-E-H-E-[KR]-E-V NAME GTP-bmding elongation factors signamre CONSENSUS D-[KRSTGANQFYW]-x(3)-E-[KRAQ]-x-[RKQD]-[GC]-[IVMK]-[ST]-[IV]-x(2)- CONSENSUS [GSTACKRNQ] NAME Elongation factor 1 beta/beta '/delta chain signamre 1 CONSENSUS [DE]-[DEG]-[DE](2)-[LIVMF]-D-L-F-G NAME Elongation factor 1 beta/beta '/delta chain signamre 2 CONSENSUS V-Q-S-x-D-[LIVM]-x-A-[FWM]-[NQ]-K-[LIVM] NAME Elongation factor 1 gamma chain profile NAME Elongation factor Ts signamre 1 CONSENSUS L-R-x(2)-T-[GDQ]-x-[GS]-[LIVMF]-x(0,l)-[DENKAC]-x-K-[KRNEQS]-[AV]-L NAME Elongation factor Ts signamre 2 CONSENSUS E-[LIVM]-N-[SCV]-[QE]-T-D-F-V-[SA]-[KRN] NAME Elongation factor P signamre CONSENSUS K-x-A-x(4)-G-x(2)-[LIV]-x-V-P-x(2)-[LIV]-x(2)-G NAME Eukaryotic initiation factor IA signamre CONSENSUS [IM]-x-G-x-[GS]-[KRH]-x(4)-[CL]-x-D-G-x(2)-R-x(2)-[RH]-I-x-G NAME Eukaryotic initiation factor 4E signamre CONSENSUS [DE]-[IFY]-x(2)-F-[KR]-x(2)-[LIVM]-x-P-x-W-E-[DV]-x(5)-G-G-[KR]-W NAME Eukaryotic initiation factor 5A hypusine signamre CONSENSUS [PTJ-G-K-H-G-x-A-K NAME Initiation factor 2 signamre CONSENSUS G-x-[LIVM]-x(2)-L-[KR]-[KRHNS]-x-K-x(5)-[LIVM]-x(2)-G-x-[DEN]-C-G NAME Initiation factor 3 signamre CONSENSUS [KR]-[LIVM](2)-[DN]-[FY]-[GSN]-[KR]-[LrVMFYS]-x-[FY]-[DEQT]-x(2)-[KR] NAME Translation initiation factor SUI1 signamre CONSENSUS [LIVM]-[EQ]-[LIVM]-Q-G-[DEN]-[KHQ]-[KRV] NAME Prokaryotic-type class I peptide chain release factors signamre CONSENSUS [AR]-[STA]-x-G-x-G-G-Q-[HNGCS]-V-N-x(3)-[ST]-A-[IV] NAME Transcription termination factor nusG signamre CONSENSUS [LIVM}-F-G-[KRW]-x-T-P-[IV]-x-[LIVM] NAME Calponin family repeat CONSENSUS [LIVM]-x-[LS]-Q-[MAS]-G-[STY]-[NT]-[KRQ]-x(2)-[STN]-Q-x-G-x(3,4)-G NAME CAP protein signamre 1 CONSENSUS [LIVM](2)-x-R-L-[DE]-x(4)-R-L-E NAME CAP protein signamre 2 CONSENSUS D-[LrVMFY]-x-E-x-[PA]-x-P-E-Q-[LIVMFY]-K NAME Calreticuhn family signamre 1 CONSENSUS [KRHN]-x-[DEQN]-[DEQNK]-x(3)-C-G-G-[AG]-[FY]-[LIVM]-[KN]-[LrVMFY](2) NAME Calreticuhn family signamre 2 CONSENSUS [LIVM](2)-F-G-P-D-x-C-[AG] NAME Calreticuhn family repeated motif signamre CONSENSUS [IV]-x-D-x-[DENST]-x(2)-K-P-[DEH]-D-W-[DEN] NAME Calsequestπn signamre 1 CONSENSUS [EQ]-[DE]-G-L-[DN]-F-P-x-Y-D-G-x-D-R-V NAME Calsequestπn signamre 2 CONSENSUS [DE]-L-E-D-W-[LIVM]-E-D-V-L-x-G-x-[LIVM]-N-T-E-D-D-D NAME S-100/ICaBP type calcium binding protein signamre CONSENSUS [LIVMFYW](2)-x(2)-[LK]-D-x(3)-[DN]-x(3)-[DNSG]-[FY]-x-[ES]-[FYVC]-x(2)- CONSENSUS [LIVMFSHLIVMF] NAME Hemolysin-type calcium-binding region signamre CONSENSUS D-x-[LI]-x(4)-G-x-D-x-[LI]-x-G-G-x(3)-D NAME HlyD family secretion proteins signamre CONSENSUS [LIVM]-x(2)-G-[LM]-x(3)-[STGAV]-x-[LIVMT]-x-[LIVMT]-[GE]-x-[KR]-x- CONSENSUS [LIVMFYW](2)-x-[LIVMFYW](3) NAME P-π protein urydylation site CONSENSUS Y-[KR]-G-[AS]-[AE]-Y NAME P-II protein C-terminal region signamre CONSENSUS [ST]-X(3)-G-[DY]-G-[KR]-[IV]-[FW]-[LΓVM]-X(2)-[LIVM] NAME 14-3-3 proteins signamre 1 CONSENSUS R-N-L-[LIV]-S-[VG]-[GA]-Y-[KN]-N-[IVA] NAME 14-3-3 proteins signamre 2 CONSENSUS Y-K-[DE]-S-T-L-I-[IM]-Q-L-[LF]-[RHC]-D-N-[LF]-T-[LS]-W-[TAN]-[SAD] NAME ATP1G1 / PLM / MAT8 family signamre CONSENSUS [DNS]-x-F-x-Y-D-x(2)-[ST]-[LIVM]-[RQ -x(2)-G NAME BTG1 family signature 1 CONSENSUS Y-x(2)-[HP]-W-[FY]-[AP]-E-x-P-x-K-G-x-[GA]-[FY]-R-C-[IV]-[RH]-[IV] NAME BTG1 family signamre 2 CONSENSUS [LV]-P-x-[DE]-[LM]-[ST]-[LIVM]-W-[IV]-D-P-x-E-V-[SC]-x-[RQ]-x-G-E NAME Cullin family signature CONSENSUS [LIV]-K-x(2)-[LIV]-x(2)-L-I-[DEQ]-[KRHNQ]-x-Y-[LIVM]-x-R-x(6,7)-[FY]-x- CONSENSUS Y-x-[SA] > NAME Cullin family profile NAME Enhancer of rudimentary signamre CONSENSUS Y-D-I-[SA]-x-L-[FY]-x-F-[IV]-D-x(3)-D-[LIV]-S NAME G10 protein signamre 1 CONSENSUS L-C-C-x-[KR]-C-x(4)-[DE]-x-N-x(4)-C-x-C-R-V-P NAME G10 protein signamre 2 CONSENSUS C-x-H-C-G-C-[KRH]-G-C-[SA] NAME Glucokinase regulatory protein family signamre CONSENSUS G-[PA]-E-x-[LIV]-[STA]-G-S-[ST]-R-[LIVM]-K-[STGA](3)-x(2)-K NAME GTP1/OBG family signamre CONSENSUS D-[LIVM]-P-G-[LIVM](2)-[DEY]-[GN]-A-x(2)-G-x-G NAME HIT family signature CONSENSUS [NQA]-x(4)-[GAV]-x-[QF]-x-[LIVM]-x-H-[LIVMFYT]-H-[LIVMFT]-H-[LIVMF](2)- CONSENSUS [PSGA] NAME Caserns alpha/beta signamre CONSENSUS C-L-[LV]-A-x-A-[LVF]-A NAME Clathπn adaptor complexes medium cham signamre 1 CONSENSUS [IVT]-[GSP]-W-R-x(2,3)-[GAD]-x(2)-[HY]-x(2)-N-x-[LIVMAFY](3)-D-[LrVM]- CONSENSUS [LIVMT]-E NAME Clathrm adaptor complexes medium cham signamre 2 CONSENSUS [LIV]-x-F-I-P-P-x-G-x-[LIVMFY]-x-L-x(2)-Y NAME Clathrm adaptor complexes small chain signamre CONSENSUS [LIVM](2)-Y-[KR]-x(4)-L-Y-F NAME Ependynuns signamre 1 CONSENSUS F-E-E-G-x-[LIVMF]-Y-[ED]-I-D-x(2)-N-[QE]-S-C-[RKH](2) NAME Ependynuns signamre 2 CONSENSUS [QE]-[LIVMA]-F-X(2)-P-[STA}-[FY]-C-[DE]-[GA]-[LΓVM]-X(2)-[DE](2) NAME Syntaxin / epimorphin family signamre CONSENSUS [RQ]-x(3)-[LIVMA]-x(2)-[LIVM]-[ESH]-x(2)-[LIVMT]-x-[DEVM]-[LIVM]-x(2)- CONSENSUS [LIVM]-[FS]-x(2)-[LIVM]-x(3)-[LIVT]-x(2)-Q-[GADEQ]-x(2)-[LIVM]-[DNQT]-x- CONSENSUS [LIVMF]-[DESV]-x(2)-[LIVM] NAME Extracellular proteins SCP/Tpx-l/Ag5/PR-l/Sc7 signamre 1 CONSENSUS [GDER]-H-[FYWH]-T-Q-[LrVM](2)-W-x(2)-[STN] NAME Extracellular proterns SCP/Tpx-l/Ag5/PR-l/Sc7 signamre 2 CONSENSUS [LIVMFYH]-[LIVMFY]-x-C-[NQRHS]-Y-x-[PARH]-x-[GL]-N-[LIVMFYWDN] NAME Fetuin family signamre 1 CONSENSUS C-x(56)-C-x(10)-C-x(13)-C-x( 17, 18)-C-x(13)-C-x(2)-C-x(58)-C-x(10, 11)- CONSENSUS C-x(10,12)-C-x(16,22)-C NAME Fetuin family signamre 2 CONSENSUS L-E-T-x-C-H-x-L-D-P-T-P NAME Legume lectins beta-chain signamre CONSENSUS [LIV]-[STAG]-V-[DEQV]-[FLI]-D-[ST] NAME Legume lectins alpha-chain signamre CONSENSUS [LIV]-x-[EDQ]-[FYWKR]-V-x-[LIV]-G-[LF]-[ST] NAME Vertebrate galactoside-bmding lectin signamre CONSENSUS W-[GEK]-x-[EQ]-x-[KRE]-x(3,6)-[PCTF]-[LIVMF]-[NQEGSKV]-x-[GH]-x(3)- CONSENSUS [DENKHS]-[LIVMFC] NAME Lysosome-associated membrane glycoproteins duplicated domain signamre CONSENSUS [STA]-C-[LIVM]-[LIVMFYW]-A-x-[LIVMFYW]-x(3)-[LIVMFYW]-x(3)-Y NAME LAMP glycoproteins transmembrane and cytoplasmic domain signamre CONSENSUS C-x(2)-D-x(3,4)-[LIVM](2)-P-[LrVM]-x-[LIVM]-G-x(2)-[LIVM] x-G-[LIVM](2)- CONSENSUS x-[LIVM](4)-A-[FY]-x-[LIVM]-x(2)-[KR]-[RH]-x( 1 ,2)-[STAG](2)-Y-[EQ] NAME Glycophoπn A signamre CONSENSUS I-I-x-[GAC]-V-M-A-G-[LIVM](2) NAME PMP-22 / EMP / MP20 family signamre 1 CONSENSUS [LrVMF](4)-[SA]-T-x(2)-[DNKS]-x-W-x(9, 13)-[LIV]-W-x(2)-C NAME PMP-22 / EMP / MP20 family signamre 2 CONSENSUS [RQ]-[AV]-x-M-[rV]-L-S-x-[Lη-x(4)-[GSA]-[LIVMF](3) NAME Oxysterol-binding protein family signamre CONSENSUS E [KQ]-x-S-H-[HR]-P-P-x-[STACF]-A NAME Yeast PIR proteins repeats signamre CONSENSUS S-Q-[IV]-[STGNH]-D-G-Q-[LIV]-Q-[AIV]-[STA] NAME Seminal vesicle protein I repeats signamre CONSENSUS [IVM]-x-G Q D-x-V K-x(5)-[KN]-G-x(3)-[STLV] NAME Seminal vesicle protein II repeats signamre CONSENSUS [GSA]-Q-x-K-S-[FY] x Q-x-K [SA] NAME Serum amyloid A proteins signamre CONSENSUS A-R-G-N-Y-[ED]-A-x-[QKR]-R-G-x-G-G-x-W-A NAME Spermadhesins family signamre 1 CONSENSUS C-G-x(2)-[LI]-x(4)-G-x-I-x(9) C-x-W-T NAME Spermadhesins family signamre 2 CONSENSUS C-x-K-E-x-[LIVM]-E-[LIVM]-x-[DE]-x(3)-[GS]-x(5)-K-x-C NAME Stress-induced proteins SRP1/TIP1 family signamre CONSENSUS P-W-Y-[ST](2)-R-L NAME Glypicans signamre CONSENSUS C-x(2)-C-x-G-[LIVM]-x(4)-P-C-x(2)-[FY]-C-x(2)-[LIVM]-x(2)-G-C NAME Syndecans signature CONSENSUS [FY]-R-[IM]-[KR]-K(2)-D-E-G-S-Y NAME Tissue factor signamre CONSENSUS W-K-x-K-C-x(2)-T-x-[DEN]-T-E-C-D-[LIVM]-T-D-E NAME Translaπonally controlled tumor protein signamre 1 CONSENSUS [IA]-G-[GAS]-N-[PA]-S-A-E-[GDE]-[PAGE]-x(0,l)-[DEG]-x-[DEN]-x(2)-[DE] NAME Translaπonally controlled tumor protein signamre 2 CONSENSUS [FL]-[FY]-[rVT]-G-E-x-[MA]-x(2,5)-[DEN]-[GAS]-x-[LV]-[AV]-x(3)-[FY]-[KR]- CONSENSUS [DE] NAME Tub family signamre 1 CONSENSUS F-[KHQ]-G-R-V-[ST]-x-A-S-V-K-N-F-Q NAME Tub family signamre 2 CONSENSUS A-F-[AG]-I-[SAC]-[LIVM]-[ST]-S-F-x-[GST]-K-x-A-C-E NAME HCP repeats signamre CONSENSUS H-R-H-R-G-H-x(2)-[DE](7) NAME: Bactenal ice-nucleation proteins octamer repeat CONSENSUS. A-G-Y-G-S-T-x-T. NAME- Cell cycle proteins ftsW / rodA / spoVE signamre. CONSENSUS [NV]-x(5)-[GTR]-[LIVMA]-x-P-[PTLIVM]-x-G-[LIVM]-x(3)-[LIVMFW](2)-S-[YSA]- CONSENSUS G-G-[STN]-[SA]. NAME: Enterobacteπal virulence outer membrane protein signamre 1 CONSENSUS G-[LIVMFY]-N-[LIVM]-K-Y-R-Y-E. NAME: Enterobacteπal virulence outer membrane protein signamre 2. CONSENSUS. [FYW]-x(2)-G-x-G-Y-[KR]-F > NAME: Hydrogenases expression/synthesis hypA family signamre CONSENSUS: F-[CSA]-[FY]-[DE]-[LIVA](2)-x(3)-[ST]-[LIVM]-x(16)-C-x(2)-C-x(12,15)- CONSENSUS. C-P-x-C. NAME: Hydrogenases expression synthesis hupF/hypC family signature. CONSENSUS < M-C-[LIV]-[GA]-[LIV]-P-x-[QKR]-[LIV]. NAME: Staphylocoagulase repeat signamre. CONSENSUS- A-R-P-x(3)-K-x-S-x-T-N-A-Y-N-V-T-T-x(2)-[DN]-G-x(3)-Y-G NAME: 11-S plant seed storage proteins signamre CONSENSUS: N-G-x-[DE](2)-x-[LIVMF]-C-[ST]-x(l 1,12)-[PAG]-D. NAME: Dehydπns signamre 1 CONSENSUS S(5)-[DE]-x-[DE]-G-x(l ,2)-G-x(0, 1)-[KR](4). NAME: Dehydπns signamre 2 CONSENSUS: [KR]-[LIM]-K-[DE]-K-[LIM]-P-G. NAME: Germin family signature. CONSENSUS. G-x(4)-H-x-H-P-x-A-x-E-[LIVM] NAME: Oleosins signamre CONSENSUS- [AG]-[ST]-x(2)-[AG]-x(2)-[LrVM]-[SAD]-T-P-[LIVMF](4)-F-S-P-[LIVM](3)- CONSENSUS. P-A NAME Small hydrophilic plant seed proteins signamre. CONSENSUS: G-IEQJ-T-V-V-P-G-G-T. NAME- Pathogenesis-related proteins Betvl family signamre CONSENSUS G-x(2)-[LrVMF]-x(4)-E-x(2)-[CSTAEN]-x(8,9)-[GND]-G-[GS]-[CS]-x(2)-K-x(4)- CONSENSUS [FY]. NAME: Pollen proteins Ole e I family signature. CONSENSUS [EQ -G-x-V-Y-C-D-T-C-R. NAME: Thaumaπn family signature CONSENSUS G-x-[GF]-x-C-x-T-[GA]-D-C-x(l ,2)-G-x(2,3)-C. NAME. Mrp family signature CONSENSUS W-x(2)-[LIVM]-D-[LrVMY](4)-D-x-P-P-G-T-[GS]-D. NAME: Glucose inhibited division protein A family signamre 1 CONSENSUS [GS]-P-x-Y-C-P-S-[LIVM]-E-x-K-[LIVM]-x-[KR]-F. NAME- Glucose inhibited division protein A family signamre 2. CONSENSUS A-G-Q-X-[NT]-G-X(2)-G-Y-X-E-[SAG](3)-[QS]-G-[LIVM](2)-A-G-[LΓVMT]-N-A. NAME NOLl/NOP2/sun family signamre. CONSENSUS- [FV]-D-[KRA]-[LIVMA]-L-x-D-[AV]-P-C-[ST]-[GA]. NAME- PET112 family signamre. CONSENSUS- [DN]-x-[DN]-R-x(3)-P-L-[LrV]-E-[LIV]-x-[ST]-x-P. NAME: Protein smpB signamre CONSENSUS: [TA]-G-[LIVM]-x-L-x-G-x-E-[LIVM]-rKQ]-[SA]-[LrVM]. NAME: Hypothetical cof family signamre 1 CONSENSUS [LIVFYAN]-[LΓVMFA]-X(2)-D-[LΓVMF]-[ND]-G-T-[LIV]-[LVY]-[STANLM]. NAME Hypothetical cof family signamre 2 CONSENSUS: [LIVMFC]-G-D-[GSANQ]-x-N-D-x(3)-[LIMFY]-x(2)-[AV]-x(2)-[GSCP]-x(2)- CONSENSUS [LMP]-x(2)-[GAS] NAME- RIO1/ZK632.3/MJ0444 family signamre. CONSENSUS- [LΓVM]-V-H-[GA]-D-L-S-E-[FY]-N-X-[LΓVM] NAME: SUA5/ycιθ/yrdC family signamre CONSENSUS- [LIVMTA](3)-[LIVMFYC]-[PG]-T-[DE]-[STA]-x-[FY]-[GA]-[LIVM]-[GS]. NAME. Uncharacteπzed protein family UPFOOOl signamre. CONSENSUS: [FW]-H-[FM]-[IV]-G-x-[LIV]-Q-x-[NKR]-K-x(3)-[LIV] NAME: Uncharacteπzed protein family UPF0003 signamre CONSENSUS: G-x-V-x(2)-[LIV]-x(3)-[SA]-x(6)-D-x(3)-[LIVT](3)-P-N-x(2)-[LIVMF](2)- CONSENSUS: x(5)-N NAME- Uncharacteπzed protein family UPF0004 signamre. CONSENSUS: [LIVM]-x-[LIVMT]-x(2)-G-C-x(3)-C-[STAN]-[FY]-C-x-[LIVM]-x(4)-G NAME- Uncharacteπzed protein family UPF0005 signamre. CONSENSUS: G-[LIVM](2)-[SA]-x(5,8)-G-x(2)-[LIVM]-G-P-x-L-x(4)-[SAG]-x(4,6)- CONSENSUS: [LIVM](2)-x(2)-A-x(3)-T-A-[LIVM](2)-F. NAME- Uncharacteπzed protein family UPF0006 signamre 1. CONSENSUS: [LIVMFY](2)-D-[STA]-H-x-H-[LIVMF]-[DN]. NAME- Uncharacteπzed protein family UPF0006 signamre 2 CONSENSUS P-[LIVM]-x-[LIVM]-H-x-R-x-[TA]-x-[DE] . NAME Uncharactenzed protein family UPF0006 signamre 3. CONSENSUS: [LVSA]-[LIVA]-x(2)-[LIVM]-[PS]-x(3)-L-[LIVM]-[LIVMS]-E-T-D-x-P NAME- Uncharactenzed protein family UPF0007 signamre. CONSENSUS. V-L-[IV]-H-D-[GA]-A-R NAME: Uncharacteπzed protein family UPF0011 signature CONSENSUS S-D-A-G-x-P-x-[LIV]-[SN]-D-P-G. NAME: Uncharactenzed protem family UPF0012 signamre. CONSENSUS [GTA]-x(2)-[IVT]-C-Y-D-[LIVM]-x-F-P-x(9)-G NAME. Uncharacteπzed protein family UPF0015 signamre. CONSENSUS: [DE]-[LIVMF](3)-R-T-[SG]-G-x(2)-R-x-S-x-[FY]-[LIVM](2)-W-Q NAME: Uncharactenzed protein family UPF0016 signamre. CONSENSUS- E-[LIVM]-G-D-K-T-F-[LIVMF](2)-A NAME- Uncharactenzed protem family UPF0017 signamre. CONSENSUS D-x(8)-[GN]-[LFY]-x(4)-[DET]-[LY]-Y-x(3)-[ST]-x(7)-[IV]-x(2)-[PS]-x- CONSENSUS: [LIVM]-x-[LIVM]-x(3)-[DN]-D. NAME: Uncharactenzed protein family UPF0019 signamre. CONSENSUS- L-P-V-[VT]-[NQL]-F-[AT]-A-G-G-[LIV]-A-T-P-A-D-A-A-[LM]. NAME: Uncharactenzed protein family UPF0020 signamre. CONSENSUS: D-P-[LIVMF]-C-G-[ST]-G-x(3)-[LI]-E. NAME: Uncharactenzed protein family UPF0021 signamre. CONSENSUS. C-K-x(2)-F-x(4)-E-x(22,23)-S-G-G-K-D. NAME: Uncharacteπzed protem family UPF0023 signamre CONSENSUS: D-x-D-E-[LIV]-L-x(4)-V-F-x(3)-S-K-G. NAME: Uncharacteπzed protem family UPF0024 signamre. CONSENSUS G-x-K-D-[KR]-x-A-[LV]-T-x-Q-x-[LIVF]-[SGC]. NAME: Uncharactenzed protem family UPF0025 signamre. CONSENSUS: D-V-[LIV]-x(2)-G-H-[ST]-H-x(12)-[LIVMF]-N-P-G. NAME Uncharactenzed protem family UPF0027 signamre. CONSENSUS: Q-[LiVM]-x-N-x-A-x-[LrVM]-P-x-I-x(6)-[LIVM]-P-D-x-H-x-G-x-G-x(2)-[IV]-G. NAME- Uncharacteπzed protem family UPF0028 signamre. CONSENSUS [GA]-[GS]-G-[GA]-A-R-G-x-[SA]-H-x-G-x(9)-[IV]-x-[IV]-D-x(2)-[GA]-G-x-S- CONSENSUS x-G NAME Uncharactenzed protein family UPF0029 signamre CONSENSUS G-x(2)-[LIVM](2)-x(2)-[LIVM]-x(4)-[LIVM]-x(5)-[LIVM](2)-x-R-[FYW](2)-G- CONSENSUS G-x(2)-[LIVM]-G Uncharactenzed protein family UPF0030 signamre CONSENSUS [GA]-L-I-[LIV]-P-G-G-E-S-T-[STA] NAME Uncharacteπzed protein family UPF0031 signature 1 CONSENSUS [SAV]-[IVW]-[LVA]-[LIV]-G-[PNS]-G-L-[GP]-x-[DENQT] NAME Uncharactenzed protein family UPF0031 signature 2 CONSENSUS [GA]-G-x-G-D-[TV]-[LT]-[STA]-G-x-[LIVM] NAME Uncharactenzed protein family UPF0032 signamre CONSENSUS Y-x(2)-F-[LIVMA](2)-x-L-x(4)-G-x(2) F-[EQ]-[LIVMF]-P-[LIVM] NAME Uncharactenzed protein family UPF0033 signamre CONSENSUS L-[DN]-x(2)-[TAG]-x(2)-C-P-x-P-x-[LIVM] NAME Uncharactenzed protein family UPF0034 signamre CONSENSUS [LIVM]-[DNG]-[LIVM]-N-x-G-C-P-x(3)-[LIVMASQ]-x(5)-G-[SAC] NAME Uncharactenzed protein family UPF0035 signamre CONSENSUS L-L-T-x-R-[SA]-x(3)-R-x(3)-G-x(3)-F-P-G-G NAME Uncharactenzed protein family UPF0O36 signamre CONSENSUS H-x-S-G-H-[GA]-x(3)-[DE]-x(3)-[LM]-x(5)-P-x(3)-[LIVM]-P-x-H-G-[DE] NAME Uncharactenzed protein family UPF0038 signamre CONSENSUS G-x-[LI]-x-R-x(2)-L-x(4)-F-x(8HLrvj-x(5)-P-x-[LIV] NAME Uncharactenzed protein family UPF0044 signamre CONSENSUS L-[ST]-x(3)-K-x(3)-[KR] [SGA]-x-[GA] H-x-L-x-P-[LrV]-x(2)-[LrV]-[GA]- CONSENSUS x(2)-G NAME Uncharactenzed protein family UPF0047 signamre CONSENSUS S-X(2)-[LIV]-X-[LΓV]-X(2)-G-X(4)-G-T-W-Q-X-[LIV] NAME Uncharacteπzed protein family UPF0054 signamre CONSENSUS H-[GS]-x-L-H-L-[LI]-G-[FYW]-D-H NAME Uncharactenzed protein family UPF0057 signamre CONSENSUS [LΓV]-X-[STA]-[LIVF](3)-P-P-[LIVA]-[GA]-[IV]-X(4)-[GKN] NAME Hypothetical YER057c/yjjV family signamre CONSENSUS P-[AT]-R-[SA]-X-[LΓVMY]-X(2)-[AK]-X-L-P-X(4)-[LIVM]-E NAME Hypothetical hesB/yadR/yfhF family signamre CONSENSUS F-x-[LIVMFY]-x-N-[PG]-[NSK]-x(4)-C-x-C-[GS]-x-S-F NAME Hypothetical yabO/yceC/sffiB family signamre CONSENSUS [NHY]-R-[LI]-D-x(2)-T-[ST]-G-[LIVMA]-[LIVMF](2)-[LIVMFG]-[SGAC]

Claims: We claim: 1. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hfbr2_16cl6; hfbr2_16f21; hfbr2_16gl8; hfbr2_16il2; hfbr2_16k22; hfbr2_16112; hfbr2_22f21; hfbr2_22hl3; hfbr2_22hl3; hfbr2_22i4; hfbr2_22k3; hfbr2_22k8; hfbr2_23bl0; hfbr2_23b21; hfbr2_23f2; hfbr2_23124; hfbr2_23nl6; hfbr2_23o24; hfbr2_23o5; hfbr2_2a2; hfbr2_2bl7; hfbr2_2b5; hfbr2_2cl; hfbr2_2cl7; hfbr2_2cl8; hfbr2_2dl5; hfbr2_2dl7; hfbr2_2d20; hfbr2_2gl8; hfbr2_2hl; hfbr2_2hl0; hfbr2_2il7; hfbr2_2kl4; hfbr2_2kl9; hfbr2_3bl6; hfbr2_3cl8; hfbr2_3fl6; hfbr2_3g8; hfbr2_312; hfbr2_41ml5; hfbr2_62bll; hfbr2_62fl0; hfbr2_62119; hfbr2_62nl0; hfbr2_62ol7; hfbr2_64al l; hfbr2_64al5; hfbr2_64cl6; hfbr2_64c4; hfbr2_64h6; hfbr2_64i20; hfbr2_64jl8; hfbr2_64k24; hfbr2_64ol6; hfbr2_6al7; hfbr2_6b24; hfbr2_6i20; hfbr2_6ol7; hfbr2_71o20; hfbr2_72bl8; hfbr2_72dl3; hfbr2_72112; hfbr2_72ml6; hfbr2_72nl2; hfbr2_78c24; hfbr2_78dl3; hfbr2_78k24; hfbr2_78n23; hfbr2_7a24; hfbr2_7e22; hfbr2_7j4; hfbr2_82c20; hfbrl_10c20; hfbr2_82el7; hfbrl_10el7; hfbr2_82e4;; hfbrl_10e4; hfbr2_82gl4;; hfbrl_10gl4; hfbr2_82il7;; hfbrl_10; hfbr2_82i24;; hfbrl lO; hfbr2_82ml6;; hfbrl_10; hfbr2_82m6;; hfbrl_10; hfkd2_lj9; hfkd2_24al5; hfkd2_24bl5; hfkd2_24e23; hfkd2_24n20; hfkd2_24p5; hfkd2_3il3; hfkd2_3ol7; hfkd2_46a6; hfkd2_46bl0; hfkd2_46dl3; hfkd2_46j20; hfkd2_46kl9; hfkd2_46m4; hfkd2_47a4; hfkd2_4b6; hfkd2_4c8; hfkd2_4kl4; hfkd2_4ml l; hmcfl lall; hmcfl_lc23; hmcfl_lel5; hmcfl_lgl3; hhtes3_ln3; htes3_14g5; htes3_14h21; htes3_14pl4; htes3_14p7; htes3_15al3; Htes3_15c24; htes3_15c6; htes3_15gl4; htes3_15hl; htes3_15i5; htes3_15jl8; Htes3_15j3; htes3_15kll; htes3_17fl0; htes3_17U7; htes3_17nl2; htes3_17nl8; Htes3_18f3; htes3_1817; htes3_19fl9; htes3_19jl7; htes3_lcl; htes3_lgl3; htes3_lkll; htes3_20c21; htes3_20k2; htes3_20ml8; htes3_21d4; htes3_21jl5; htes3_21116; htes3_21n23; htes3_22c23; htes3_22g2; htes3_22nl3; htes3_23111; htes3_23nl9; Htes3_23nl9; htes3_26g22; htes3_27dl; htes3_27k4; htes3_27ol4; htes3_28dl4; htes3_2al l; htes3_2al7; htes3_2dl5; htes3_2el2; htes3_2fl4; htes3_2g7; htes3_2hl; htes3_2hl5; htes3_2119; htes3_2ml8; htes3_2m20; htes3_2n9; htes3_2ol3; htes3_30f4; Htes3_35b4; htes3_35b5; htes3_35e21; htes3_35g6; htes3_35kl6; htes3_35k24; htes3_35nl2; htes3_35n24; htes3_35n9; htes3_35pl7; htes3_35p22; htes3_4b4; htes3_4fl7; htes3_4f5; htes3_4h6; htes3_4ol9; htes3_50j4; htes3_50n06; htes3_50n23; htes3_6b21; htes3_6cll; htes3_6dl6; htes3_72kl l; Htes3_72kl5; htes3_72pl6; htes3_7b22; htes3_7dl7; htes3_7j3; htes3_7j8; htes3_7pl0; htes3_7p9; htes3_8e24; Htes3_8gl l; Htes3_8g5; htes3_8ml0; Htes3_8p7; Htes3_9e22; Htes3_9i20; Htes3_9k22; hutel_17k7; hutel_18cl2; hutel_18il9; hutel_18i4; hutel lδll; hutel_19fl9; hutel_19gl9; hutel_19g22; hutel_19hl7; hutel_19jll; hutel_li2; hutel_20bl9; hutel_20g21; hutel_20hl3; hutel_20mll; hutel_20m24; hutel_21dl5; hutel_22d2; hutel_22el2; hutel_22n2; hutel_22o2; hutel_23el3; hutel_23gll; hutel_24cl9; hutel_24ell; hutel_24j6; hutel_2h3; their complements; and variants thereof. 2. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hfbr2_16cl6; hfbr2_16f21; hfbr2_16gl8; hfbr2_16il2; hfbr2_16k22; hfbr2_16112; hfbr2_22f21; hfbr2_22hl3; hfbr2_22hl3; hfbr2_22i4; hfbr2_22k3; hfbr2_22k8; hfbr2_23bl0; hfbr2_23b21; hfbr2_23f2; hfbr2_23124; ; hfbr2_23nl6; hfbr2_23o24; hfbr2_23o5; hfbr2_2a2; hfbr2_2bl7; hfbr2_2b5; hfbr2_2cl; hfbr2_2cl7; hfbr2_2cl8; hfbr2_2dl5; hfbr2_2dl7; hfbr2_2d20; hfbr2_2gl8; hfbr2_2hl; hfbr2_2hlO; hfbr2_2il7; hfbr2_2kl4; hfbr2_2kl9; hfbr2_3cl8; hfbr2_3fl6; hfbr2_3g8; hfbr2_312; hfbr2_41ml5; hfbr2_62bll; hfbr2_62fl0; hfbr2_62119; hfbr2_62nl0; hfbr2_62ol7; hfbr2_64all; hfbr2_64al5; hfbr2_64cl6; hfbr2_64c4; hfbr2_64h6; hfbr2_64i20; hfbr2_64jl8; hfbr2_64k24; hfbr2_64ol6; hfbr2_6al7; hfbr2_6b24; hfbr2_6i20; hfbr2_6ol7; hfbr2_71o20; hfbr2_72bl8; hfbr2_72dl3; hfbr2_72112; hfbr2_72ml6; hfbr2_72nl2; hfbr2_78c24; hfbr2_78dl3; hfbr2_78k24; hfbr2_78n23; hfbr2_7a24; hfbr2_7e22; hfbr2_7j4; hfbr2_82c20; hfbrl_10c20; hfbr2_82el7; hfbrl_10el7; hfbr2_82e4; hfbrl_10e4; hfbr2_82gl4; hfbrl_10gl4; hfbr2_82il7; hfbrl lO; hfbr2_82i24; hfbrl lO; hfbr2_82ml6; hfbrl lO; hfbr2_82m6; hfbrl lO; their complements; and variants thereof. 3. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hfbr2_16f21; hfbr2_16k22; hfbr2_22f21; hfbr2_22hl3; hfbr2_22i4; hfbr2_22k3; hfbr2_22k8; hfbr2_23f2; ; hfbr2_23o24; hfbr2_23o5; hfbr2_2a2; hfbr2_2cl; hfbr2_2cl8; hfbr2_2d20; hibr2_2gl8; hfbr2_2hl; hfbr2_2hl0; hfbr2_2kl9; hfbr2_3fl6; hfbr2_312; hfbr2_62nl0; hfbr2_64al 1; hfbr2_64cl6; hfbr2_64c4; hfbr2_64h6; hfbr2_64i20; hfbr2_64ol6; hfbr2_6al7; hfbr2_6i20; hfbr2_71o20; hfbr2_72dl3; hfbr2_72ml6; hfbr2_72nl2; hfbr2_78dl3; hfbr2_78n23; hfbr2_7a24; hfbr2_7e22; hfbr2_7j4; hfbr2_82ml6; and hfbrl_10. 4. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hfkd2_lj9; hfkd2_24al5; hfkd2_24bl5; hfkd2_24e23; hfkd2_24n20; hfkd2_24p5; hfkd2_3il3; hfkd2_3ol7; hfkd2_46a6; hfkd2_46bl0; hfkd2_46dl3; hfkd2_46j20; hfkd2_46kl9; hfkd2_46m4; hfkd2_47a4; hfkd2_4b6; hfkd2_4c8; hfkd2_4kl4; hfkd2_4mll; their complements; and variants thereof. 5. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hfkd2_lj9; hfkd2_24e23; hfkd2_46a6; hfkd2_46bl0; hfkd2_46dl3; hfkd2_4b6; hfkd2_4c8; their complements; and variants thereof. 6. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hmcfl_lall; hmcfl_lc23; hmcfl_lel5; hmcfl_lgl3; their complements; and variants thereof. 7. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hmcfl_lc23 hmcfl_lgl3; their complements; and variants thereof. 8. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hhtes3_ln3; htes3_14g5; htes3_14h21; htes3_14pl4; htes3_14p7; htes3_15al3; Htes3_15c24; htes3_15c6; htes3_15gl4; htes3_15hl; htes3_15i5; htes3_15jl8; Htes3_15j3; htes3_15kll; htes3_17fl0; htes3_17117; htes3_17nl2; htes3_17nl8; Htes3_18f3; htes3_1817; htes3_19fl9; htes3_19jl7; htes3_lcl; htes3_lgl3; htes3_lkll; htes3_20c21; htes3_20k2; htes3_20ml8; htes3_21d4; htes3_21jl5; htes3_21116; htes3_21n23; htes3_22c23; htes3_22g2; htes3_22nl3; htes3_23111; htes3_23nl9; Htes3_23nl9; htes3_26g22; htes3_27dl; htes3_27k4; htes3_27ol4; htes3_28dl4; htes3_2al l; htes3_2al7; htes3_2dl5; htes3_2el2; htes3_2fl4; htes3_2g7; htes3_2hl; htes3_2hl5; htes3_2119; htes3_2ml8; htes3 2m20; htes3 2n9; htes3 2ol3; htes3 30f4; Htes3 35b4; htes3 35b5; htes3 35e21; htes3_35g6; htes3_35kl6; htes3_35k24; htes3_35nl2; htes3_35n24; htes3_35n9; htes3_35pl7; htes3_35p22; htes3_4b4; htes3_4fl7; htes3_4f5; htes3_4h6; htes3_4ol9; htes3_50j4; htes3_50n06; htes3_50n23; htes3_6b21; htes3_6cll; htes3_6dl6; htes3_72kll; Htes3_72kl5; htes3_72pl6; htes3_7b22; htes3_7dl7; htes3_7j3; htes3_7j8; htes3_7pl0; htes3_7p9; htes3_8e24; Htes3_8gll; Htes3_8g5; htes3_8ml0; Htes3_8p7; Htes3_9e22; Htes3_9i20; Htes3_9k22; their complements; and variants thereof. 9. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: htes3_14g5; htes3_14pl4; htes3_14p7; htes3_15al3; htes3_15gl4; htes3_15hl ; htes3_15jl8; htes3_17fl0; Htes3_18f3; htes3_19fl9; htes3_19jl7; htes3_20c21; htes3_21n23; htes3_22c23; htes3_22nl3; Htes3_23nl9; htes3_27ol4; htes3_28dl4; htes3_2al l; htes3_2dl5; htes3_2Ω4; htes3_2g7; htes3_2hl5; htes3_2119; htes3_2m20; htes3_2n9; htes3_30f4; htes3_35g6; htes3_35n24; htes3_35pl7; htes3_4b4; htes3_4Ω7; htes3_4ol9; htes3_50j4; htes3_50n23; htes3_50n06; htes3_6b21; htes3_6dl6; htes3_72kl 1; htes3_7dl7; htes3_7j8; Htes3_8gl 1; Htes3_8g5; Htes3_8p7; Htes3_9e22; Htes3_9i20; Htes3_9k22; their complements; and variants thereof. 10. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hfbr2_16gl8; hfbr2_2kl4; Htes3_35b4; htes3_35p22; htes3_7j3; htes3_7pl0; hutel_20ml l; their complements; and variants thereof. 11. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hfbr2_16cl6; hfbr2_2b5; htes3_15i5; htes3_1817; htes3_lkll; Htes3_72kl5; htes3_7b22; hutel_19g22; hutel_24j6; their complements; and variants thereof. 12. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hfbr2_2dl5; htes3_35e21; hutel_2h3; their complements; and variants thereof. 13. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hfbr2_23124; hfbr2_2il7; hfbr2_41ml5; hfbr2_62fl0; hfbr2_62119; hfbr2_64jl8; hfkd2_24n20; hfkd2_24p5; hfkd2_4kl4; htes3_lgl3; htes3_21116; htes3_23111; htes3_26g22; htes3_4h6; htes3_72pl6; hutel_19hl7; hutel_20hl3; hutel_24el l; their complements; and variants thereof. 14. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hfbr2_3g8; hfbr2_62ol7; hfbr2_6b24; hfbr2_78k24; hfkd2_24bl5; hfkd2_3ol7; hfkd2_46j20; htes3_17117; htes3_17nl8; htes3_27dl; htes3_2al7; htes3_35b5; htes3_35kl6; htes3_35nl2; htes3_35n9; hutel_20bl9; hutel_20m24; hutel_23el3; their complements; and variants thereof. 15. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hfbr2_23bl0; hfbr2_3cl8; hfbr2_64al5; hfbr2_6ol7; hfbr2_72bl8; hfbr2_72112; hfbr2_82i24(hfbrl_10)i htes3_14h21; Htes3_15j3; htes3_20ml8; htes3_22g2; htes3_2ml8; htes3_7p9; htes3_8ml0; hutel_1811; their complements; and variants thereof. 16. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hfbr2_23b21; hfbr2_23nl6; hfbr2_2cl7; hfbr2_62bll; hfbr2_78c24; hfbr2_82e4 (hfbrl_10e4); hfbr2_82il7 (hfbrl_10); hfbr2_82m6 (hfbrl_10);_hfkd2_46m4; htes3_15kll; htes3_lcl; hhtes3_ln3; htes3_20k2; htes3_21d4; htes3_23nl9; htes3_4f5; htes3_6cll; htes3_8e24; hutel_20g21; hutel_22d2; hutel_22el2; their complements; and variants thereof. 17. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hfbr2_16il2; hfbr2_ 16112; hfbr2_22hl3; hfbr2_2bl7; hfbr2_2dl7; hfbr2_64k24; hfbr2_82c20 (hfbrl_10c20); hfbr2_82el7 (hfbrl_10el7); hfbr2_82gl4 (hfbrl_10gl4); hfkd2_24al5; hfkd2_3il3; hfkd2_4mll; hmcfl_lall; hmcfl_lel5; htes3_15c6; htes3_2ol3; htes3_27k4; htes3_2hl; htes3_35k24; hutel_19fl9; and hutel_24cl9; their complements; and variants thereof. 18. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hfkd2_46kl9; hfkd2_47a4; htes3_2el2; htes3_21jl5; htes3_17nl2; hutel_18il9; hutel_H2; their complements; and variants thereof. 19. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hutel_17k7; hutel_18cl2; hutel_18il9; hutel_18i4; hutel_1811; hutel_19fl9; hutel_19gl9; hutel_19g22; hutel_19hl7; hutel_19jll; hutel_li2; hutel_20bl9; hutel_20g21; hutel_20hl3; hutel_20mll; hutel_20m24; hutel_21dl5; hutel_22d2; hutel_22el2; hutel_22n2; hutel_22o2; hutel_23el3; hutel_23gll; hutel_24cl9; hutel_24ell; hutel_24j6; hutel_2h3; their complements; and variants thereof. 20. An assemblage, comprising at least one nucleic acid molecule having the sequence of a clone selected from the group consisting of: hutel_17k7; hutel_18cl2; hutel_18i4; hutel_19gl9; hutel_19jl l; hutel _22n2; hutel_21dl5; hutel_22o2; hutel_23gl l ; their complements; and variants thereof. 21. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hfbr2_16cl6; hfbr2_16f21; hfbr2_16gl8; hfbr2_16il2; hfbr2_16k22; hfbr2_16112; hfbr2_22f21; hfbr2_22hl3; hfbr2_22hl3; hfbr2_22i4; hfbr2_22k3; hfbr2_22k8; hfbr2_23bl0; hfbr2_23b21; hfbr2_23f2; hfbr2_23124; ; hfbr2_23nl6; hfbr2_23o24; hfbr2_23o5; hfbr2_2a2; hfbr2_2bl7; hfbr2_2b5; hfbr2_2cl; hfbr2_2cl7; hfbr2_2cl8; hfbr2_2dl5; hfbr2_2dl7; hfbr2_2d20; hfbr2_2gl8; hfbr2_2hl; hfbr2_2hl0; hfbr2_2il7; hfbr2_2kl4; hfbr2_2kl9; hfbr2_3cl8; hfbr2_3fl6; hfbr2_3g8; hfbr2_312; hfbr2_41ml5; hfbr2_62bl l; hfbr2_62Ω0; hfbr2_62119; hfbr2_62nl0; hfbr2_62ol7; hfbr2_64al l; hfbr2_64al5; hfbr2_64cl6; hfbr2_64c4; hfbr2_64h6; hfbr2_64i20; hfbr2_64jl8; hfbr2_64k24; hfbr2_64ol6; hfbr2_6al7; hfbr2_6b24; hfbr2_6i20; hfbr2_6ol7; hfbr2_71o20; hfbr2_72bl8; hfbr2_72dl3; hfbr2_72112; hfbr2_72ml6; hfbr2_72nl2; hfbr2_78c24; hfbr2_78dl3; hfbr2_78k24; hfbr2_78n23; hfbr2_7a24; hfbr2_7e22; hfbr2_7j4; hfbr2_82c20; hfbrl_10c20; hfbr2_82el7; hfbrl_10el7; hfbr2_82e4;; hfbrl_10e4; hfbr2_82gl4;; hfbrl_10gl4; hfbr2_82il7;; hfbrl lO; hfbr2_82i24;; hfbrl_10; hfbr2_82ml6;; hfbrl_10; hfbr2_82m6;; hfbrl_10; hfkd2_lj9; hfkd2_24al5; hfkd2_24bl5; hfkd2_24e23; hfkd2_24n20; hfkd2_24p5; hfkd2_3il3; hfkd2_3ol7; hfkd2_46a6; hfkd2_46bl0; hfkd2_46dl3; hfkd2_46j20; hfkd2_46kl9; hfkd2_46m4; hfkd2_47a4; hfkd2_4b6; hfkd2_4c8; hfkd2_4kl4; hfkd2_4mll; hmcfl lall; hmcfl_lc23; hmcfl_lel5; hmcfl_lgl3; hhtes3_ln3; htes3_14g5; htes3_14h21; htes3_14pl4; htes3_14p7; htes3_15al3; Htes3_15c24; htes3_15c6; htes3_15gl4; htes3_15hl; htes3_15i5; htes3_15jl8; Htes3_15j3; htes3_15kll; htes3_17fl0; htes3_17117; htes3_17nl2; htes3_17nl8; Htes3_18f3; htes3_1817; htes3_19fl9; htes3_19jl7; htes3_lcl; htes3_lgl3; htes3_lkl l; htes3_20c21; htes3_20k2; htes3_20ml8; htes3_21d4; htes3_21jl5; htes3_21116; htes3_21n23; htes3_22c23; htes3_22g2; htes3_22nl3; htes3_23111; htes3_23nl9; Htes3_23nl9; htes3_26g22; htes3_27dl; htes3_27k4; htes3_27ol4; htes3_28dl4; htes3_2all; htes3_2al7; htes3_2dl5; htes3_2el2; htes3_2fl4; htes3_2g7; htes3_2hl; htes3_2hl5; htes3_2119; htes3_2ml8; htes3_2m20; htes3_2n9; htes3_2ol3; htes3_30f4; Htes3_35b4; htes3_35b5; htes3_35e21 ; htes3_35g6; htes3_35kl6; htes3_35k24; htes3_35nl2; htes3_35n24; htes3_35n9; htes3_35pl7; htes3_35p22; htes3_4b4; htes3_4fl7; htes3_4f5; htes3_4h6; htes3_4ol9; htes3_50j4; htes3_50n06; htes3_50n23; htes3_6b21; htes3_6cll; htes3_6dl6; htes3_72kl l; Htes3_72kl5; htes3_72pl6; htes3_7b22; htes3_7dl7; htes3_7j3; htes3_7j8; htes3_7pl0; htes3_7p9; htes3_8e24; Htes3_8gll; Htes3_8g5; htes3_8mlO; Htes3_8ρ7; Htes3_9e22; Htes3_9i20; Htes3_9k22; hutel_17k7; hutel_18cl2; hutel_18il9; hutel_18i4; hutel lδll; hutel_19fl9; hutel_19gl9; hutel_19g22; hutel_19hl7; hutel_19jll; hutel_H2; hutel_20bl9; hutel_20g21; hutel_20hl3; hutel_20mll; hutel_20m24; hutel_21dl5; hutel_22d2; hutel_22el2; hutel_22n2; hutel_22o2; hutel_23el3; hutel_23gll; hutel_24cl9; hutel_24ell; hutel_24j6; hutel_2h3; their complements; and variants thereof. 22. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hfbr2_16cl6; hfbr2_16f21; hfbr2_16gl8; hfbr2_16il2; hfbr2_16k22; hfbr2_16112; hfbr2_22f21; hfbr2_22hl3; hfbr2_22hl3; hfbr2_22i4; hfbr2_22k3; hfbr2_22k8; hfbr2_23bl0; hfbr2_23b21; hfbr2_23f2; hfbr2_23124; ; hfbr2_23nl6; hfbr2_23o24; hfbr2_23o5; hfbr2_2a2; hfbr2_2bl7; hfbr2_2b5; hfbr2_2cl; hfbr2_2cl7; hfbr2_2cl8; hfbr2_2dl5; hfbr2_2dl7; hfbr2_2d20; hfbr2_2gl8; hfbr2_2hl; hfbr2_2hl0; hfbr2_2il7; hfbr2_2kl4; hfbr2_2kl9; hfbr2_3cl8; hfbr2_3fl6; hfbr2_3g8; hfbr2_312; hfbr2_41ml5; hfbr2 62bll; hfbr2 62fl0; hfbr2 62119; hfbr2 62nl0; hfbr2 62ol7; hfbr2 64all; hfbr2_64al5; hfbr2_64cl6; hfbr2_64c4; hfbr2_64h6; hfbr2_64i20; hfbr2_64jl8; hfbr2_64k24; hfbr2_64ol6; hfbr2_6al7; hfbr2_6b24; hfbr2_6i20; hfbr2_6ol7; hfbr2_71o20; hfbr2_72bl8; hfbr2_72dl3; hfbr2_72112; hfbr2_72ml6; hfbr2_72nl2; hfbr2_78c24; hfbr2_78dl3; hfbr2_78k24; hfbr2_78n23; hfbr2_7a24; hfbr2_7e22; hfbr2_7j4; hfbr2_82c20; hfbrl_10c20; hfbr2_82el7; hfbrl_10el7; hfbr2_82e4; hfbrl_10e4; hfbr2_82gl4; hfbrl_10gl4; hfbr2_82il7; hfbrl lO; hfbr2_82i24; hfbrl_10; hfbr2_82ml6; hfbrl lO; hfbr2_82m6; hfbrl lO; complements of the nucleic acid sequences; and variants thereof. 23. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hfbι2_16£21; hfbr2_16k22; hfbr2_22f21; hfbr2_22hl3; hfbr2_22i4; hfbr2_22k3; hfbr2_22k8; hfbr2_23f2; ; hfbr2_23o24; hfbr2_23o5; hfbr2_2a2; hfbr2_2cl; hfbr2_2cl8; hfbr2_2d20; hfbr2_2gl8; hfbι2_2hl; hfbr2_2hl0; hfbr2_2kl9; hfbr2_3fl6; hfbr2_312; hfbr2_62nl0; hfbι2_64al 1; hfbr2_64cl6; hfbr2_64c4; hfbr2_64h6; hfbr2_64i20; hfbr2_64k24; hfbr2_64ol6; hfbr2_6al7; hfbr2_6i20; hfbr2_71o20; hfbr2_72dl3; hfbr2_72ml6; hfbr2_72nl2; hfbr2_78dl3; hfbr2_78n23; hfbr2_7a24; hfbr2_7e22; hfbr2_7j4; hfbr2_82ml6; hfbrl lO; complements of the nucleic acid sequences; and variants thereof. 24. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hfkd2_lj9; hfkd2_24al5; hfkd2_24bl5; hfkd2_24e23; hfkd2_24n20; hfkd2_24p5; hfkd2_3il3; hfkd2_3ol7; hfkd2_46a6; hfkd2_46bl0; hfkd2_46dl3; hfkd2_46j20; hfkd2_46kl9; hfkd2_46m4; hfkd2_47a4; hfkd2_4b6; hfkd2_4c8; hfkd2_4kl4; hfkd2_4mll; complements of the nucleic acid sequences; and variants thereof. 25. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hfkd2_lj9; hfkd2_24e23; hfkd2_46a6; hfkd2_46bl0; hfkd2_46dl3; hfkd2_4b6; hfkd2_4c8; complements of the nucleic acid sequences; and variants thereof. 26. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hmcfl_lall; hmcfl_lc23; hmcfl_lel5; hmcfl_lgl3; complements of the nucleic acid sequences; and variants thereof. 27. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hmcfl_lc23; hmcfl_lgl3; complements of the nucleic acid sequences; and variants thereof. 28. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hhtes3_ln3; htes3_14g5; htes3_14h21; htes3_14pl4; htes3_14ρ7; htes3_15al3; Htes3_15c24; htes3_15c6; htes3_15gl4; htes3_15hl; htes3_15i5; htes3_15jl8; Htes3_15j3; htes3_15kl l; htes3_17fl0; htes3_17117; htes3_17nl2; htes3_17nl8; Htes3_18f3; htes3_1817; htes3_19fl9; htes3_19jl7; htes3_lcl ; htes3_lgl3; htes3_lkll; htes3_20c21; htes3_20k2; htes3_20ml8; htes3_21d4; htes3_21jl5; htes3_21116; htes3_21n23; htes3_22c23; htes3_22g2; htes3_22nl3; htes3_23111; htes3_23nl9; Htes3_23nl9; htes3_26g22; htes3_27dl; htes3_27k4; htes3_27ol4; htes3_28dl4; htes3_2all; htes3_2al7; htes3_2dl5; htes3_2el2; htes3_2fl4; htes3_2g7; htes3_2hl; htes3_2hl5; htes3_2119; htes3_2ml8; htes3_2m20; htes3_2n9; htes3_2ol3; htes3_30f4; Htes3_35b4; htes3_35b5; htes3_35e21; htes3_35g6; htes3_35kl6; htes3_35k24; htes3_35nl2; htes3_35n24; htes3_35n9; htes3_35pl7; htes3_35p22; htes3_4b4; htes3_4fl7; htes3_4f5; htes3_4h6; htes3_4ol9; htes3_50j4; htes3_50n06; htes3_50n23; htes3_6b21; htes3_6cl l; htes3_6dl6; htes3_72kl l; Htes3_72kl5; htes3_72pl6; htes3_7b22; htes3_7dl7; htes3_7j3; htes3_7j8; htes3_7pl0; htes3_7p9; htes3_8e24; Htes3_8gll; Htes3_8g5; htes3_8mlO; Htes3_8p7; Htes3_9e22; Htes3_9i20; Htes3_9k22; complements of the nucleic acid sequences; and variants thereof. 29. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: htes3_14g5; htes3_14pl4; htes3_14p7; htes3_15al3; htes3_15gl4; htes3_15hl; htes3_15jl8; htes3_17fl0; htes3_17nl8; Htes3_18D; htes3_19Ω9; htes3_19jl7; htes3_20c21; htes3_21n23; htes3_22c23; htes3_22nl3; Htes3_23nl9; htes3_27ol4; htes3_28dl4; htes3_2al l; htes3_2dl5; htes3_2Ω4; htes3_2g7; htes3_2hl5; htes3_2119; htes3_2m20; htes3_2n9; htes3_30f4; htes3_35g6; htes3_35n24; htes3_35pl7; htes3_4b4; htes3_4fl7; htes3_4ol9; htes3_50j4; htes3_50n23; htes3_50n06; htes3_6b21; htes3_6dl6; htes3_72kl l; htes3_7dl7; htes3_7j8; Htes3_8gl 1 ; Htes3_8g5; Htes3_8p7; Htes3_9e22; Htes3_9i20; Htes3_9k22; complements of the nucleic acid sequences; and variants thereof. 30. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hfbr2_16gl8; hfbr2_2kl4; Htes3_35b4; htes3_35p22; htes3_7j3; htes3_7pl0; hutel_20mll; complements of the nucleic acid sequences; and variants thereof. 31. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hfbr2_16cl6; hfbr2_2b5; htes3_15i5; htes3_1817; htes3_lkll; Htes3_72kl5; htes3_7b22; hutel_19g22; hutel_24j6; complements of the nucleic acid sequences; and variants thereof. 32. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hfbr2_2dl5; htes3_35e21; hutel_2h3; complements of the nucleic acid sequences; and variants thereof. 33. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hfbr2_23124; hfbr2_2il7; hfbr2_41ml5; hfbr2_62fl0; hfbr2_62119; hfbr2_64jl8; hfkd2_24n20; hfkd2_24p5; hfkd2_4kl4; htes3_lgl3; htes3_21116; htes3_23111; htes3_26g22; htes3_4h6; htes3_72pl6; hutel_19hl7; hutel_20hl3; hutel_24ell; complements of the nucleic acid sequences; and variants thereof. 34. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hfbr2_3g8; hfbr2_62ol7; hfbr2_6b24; hfbr2_78k24; hfkd2_24bl5; hfkd2_3ol7; hfkd2_46j20; htes3_17117; Htes3_17nl8; htes3_27dl; htes3_2al7; htes3_35b5; htes3_35kl6; htes3_35nl2; htes3_35n9; hutel_20bl9; hutel_20m24; hutel_23el3; complements of the nucleic acid sequences; and variants thereof. 35. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hfbr2_23bl0; hfbr2_3cl8; hfbr2_64al5; hfbr2_6ol7; hfbr2_72bl8; hfbr2_72112; hfbr2_82i24(hfbrl_10)i_htes3_14h21; Htes3_15j3; htes3_20ml8; htes3_22g2; htes3_2ml8; htes3_7p9; htes3_8ml0; hutel_1811; complements of the nucleic acid sequences; and variants thereof. 36. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hfbr2_23b21; hfbr2_23nl6; hfbr2_2cl7; hfbr2_62bl l; hfbr2_78c24; hfbr2_82e4 (hfbrl_10e4); hfbr2_82il7 (hfbrl_10); hfbr2_82m6 (hfbrl_10);_hfkd2_46m4; htes3_15kll; htes3_lcl; hhtes3_ln3; htes3_20k2; htes3_21d4; htes3_23nl9; htes3_4f5; htes3_6cl l; htes3_8e24; hutel_20g21; hutel_22d2; hutel_22el2; complements of the nucleic acid sequences; and variants thereof. 37. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hfbr2_16il2; hfbr2_16112; hfbr2_22hl3; hfbr2_2bl7; hfbr2_2dl7; hfbr2_64k24; hfbr2_82c20 (hfbrl_10c20);_hfbr2_82el7 (hfbrl_10el7); hfbr2_82gl4 (hfbrl_10gl4); hfkd2_24al5; hfkd2_3il3; hfkd2_4mll; hmcfl_lall; hmcfl_lel5; htes3_15c6; htes3_2ol3; htes3_27k4; htes3_2hl; htes3_35k24; hutel_19fl9; and hutel_24cl9; complements of the nucleic acid sequences; and variants thereof. 38. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hfkd2_46kl9; hfkd2_47a4; htes3_2el2; htes3_21jl5; htes3_17nl2; hutel_18il9; hutel_li2; complements of the nucleic acid sequences; and variants thereof. 39. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hutel_17k7; hutel_18cl2; hutel_18il9; hutel_18i4; hutel_1811; hutel_19fl9; hutel_19gl9; hutel_19g22; hutel_19hl7; hutel_19jll; hutel_li2; hutel_20bl9; hutel_20g21; hutel_20hl3; hutel_20ml l; hutel_20m24; hutel_21dl5; hutel_22d2; hutel_22el2; hutel_22n2; hutel_22o2; hutel_23el3; hutel_23gll; hutel_24cl9; hutel_24ell; hutel_24j6; hutel_2h3; complements of the nucleic acid sequences; and variants thereof. 40. A computer readable medium, comprising in electronic form at least one nucleic acid or protein sequence of a clone selected from the group consisting of: hutel_17k7; hutel_18cl2; hutel_18i4; hutel_19gl9; hutel_19j l l ; hutel _22n2; hutel_21dl5; hutel_22o2; hutel_23gl l ; complements of the nucleic acid sequences; and variants thereof. 41. A nucleic acid molecule having the sequence of a clone selected from the group consisting of hfbr2_16cl6; hfbr2_16f21; hfbr2_16gl8; hfbr2_16il2; hfbr2_16k22; hfbr2_16112; hfbr2_22f21; hfbr2_22hl3; hfbr2_22hl3; hfbr2_22i4; hfbr2_22k3; hfbr2_22k8; hfbr2_23bl0; hfbr2_23b21; hfbr2_23f2; hfbr2_23124; hfbr2_23nl6; hfbr2_23o24; hfbr2_23o5; hfbr2_2a2; hfbr2_2bl7; hfbr2_2b5; hfbr2_2cl; hfbr2_2cl7; hfbr2_2cl8; hfbr2_2dl5; hfbr2_2dl7; hfbr2_2d20; hfbr2_2gl8; hfbr2_2hl; hfbr2_2hl0; hfbr2_2il7; hfbr2_2kl4; hfbr2_2kl9; hfbr2_3bl6; hfbr2_3cl8; hfbr2_3fl6; hfbr2_3g8; hfbr2_312; hfbr2_41ml5; hfbr2_62bll; hfbr2_62fl0; hfbr2_62119; hfbr2_62nl0; hfbr2_62ol7; hfbr2_64all; hfbr2_64al5; hfbr2_64cl6; hfbr2_64c4; hfbr2_64h6; hfbr2_64i20; hfbr2_64jl8; hfbr2_64k24; hfbr2_64ol6; hfbr2_6al7; hfbr2_6b24; hfbr2_6i20; hfbr2_6ol7; hfbr2_71o20; hfbr2_72bl8; hfbr2_72dl3; hfbr2_72112; hfbr2_72ml6; hfbr2_72nl2; hfbr2_78c24; hfbr2_78dl3; hfbr2_78k24; hfbr2_78n23; hfbr2_7a24; hfbr2_7e22; hfbr2_7j4; hfbr2_82c20; hfbrl_10c20; hfbr2_82el7; hfbrl_10el7; hfbr2_82e4;; hfbrl_10e4; hfbr2_82gl4;; hfbrl_10gl4; hfbr2_82il7;; hfbrl_10; hfbr2_82i24;; hfbrl lO; hfbr2_82ml6;; hfbrl_10; hfbr2_82m6;; hfbrl_10; hfkd2_lj9; hfkd2_24al5; hfkd2_24bl5; hfkd2_24e23; hfkd2_24n20; hfkd2_24p5; hfkd2_3il3; hfkd2_3ol7; hfkd2_46a6; hfkd2_46bl0; hfkd2_46dl3; hfkd2_46j20; hfkd2_46kl9; hfkd2_46m4; hfkd2_47a4; hfkd2_4b6; hfkd2_4c8; hfkd2_4kl4; hfkd2_4ml l; hmcfl lall; hmcfl_lc23; hmcfl_lel5; hmcfl_lgl3; hhtes3_ln3; htes3_14g5; htes3_14h21; htes3_14pl4; htes3_14p7; htes3_15al3; Htes3_15c24; htes3_15c6; htes3_15gl4; htes3_15hl; htes3_15i5; htes3_15jl8; Htes3_15j3; htes3_15kll; htes3_17fl0; htes3_17117; htes3_17nl2; htes3_17nl8; Htes3_18f3; htes3_1817; htes3_19fl9; htes3_19jl7; htes3_lcl; htes3_lgl3; htes3_lkll; htes3_20c21; htes3_20k2; htes3_20ml8; htes3_21d4; htes3_21jl5; htes3_21116; htes3_21n23; htes3_22c23; htes3_22g2; htes3_22nl3; htes3_23111; htes3_23nl9; Htes3_23nl9; htes3_26g22; htes3_27dl; htes3_27k4; htes3_27ol4; htes3_28dl4; htes3_2all; htes3_2al7; htes3_2dl5; htes3_2el2; htes3_2fl4; htes3_2g7; htes3_2hl; htes3_2hl5; htes3_2119; htes3_2ml8; htes3_2m20; htes3_2n9; htes3_2ol3; htes3_30f4; Htes3_35b4; htes3_35b5; htes3_35e21; htes3_35g6; htes3_35kl6; htes3_35k24; htes3_35nl2; htes3_35n24; htes3_35n9; htes3_35pl7; htes3_35p22; htes3_4b4; htes3_4fl7; htes3_4f5; htes3_4h6; htes3_4ol9; htes3_50j4; htes3_50n06; htes3_50n23; htes3_6b21; htes3_6cl l; htes3_6dl6; htes3_72kll; Htes3_72kl5; htes3_72pl6; htes3_7b22; htes3_7dl7; htes3_7j3; htes3_7j8; htes3_7pl0; htes3_7p9; htes3_8e24; Htes3_8gll; Htes3_8g5; htes3_8ml0; Htes3_8p7; Htes3_9e22; Htes3_9i20; Htes3_9k22; hutel_17k7; hutel_18cl2; hutel_18il9; hutel_18i4; hutel_1811; hutel_19fl9; hutel_19gl9; hutel_19g22; hutel_19hl7; hutel_19jl l; hutel_li2; hutel_20bl9; hutel_20g21; hutel_20hl3; hutel_20mll; hutel_20m24; hutel_21dl5; hutel_22d2; hutel_22el2; hutel_22n2; hutel_22o2; hutel_23el3; hutel_23gll; hutel_24cl9; hutel_24ell; hutel_24j6; hutel_2h3; their complements; and variants thereof. 42. A polypeptide encoded by the nucleic acid molecule according to claim 41. 43. An antibody or fragment thereof that is capable of binding to a specific portion of the peptide according to claim 42. 44. A pharmaceutical composition, comprising (a) an effective amount of a pharmaceutical agent, wherein said pharmaceutical agent is selected from the group consisting of the polypeptide according to claim 42, variants or functional derivatives thereof, and antibodies thereto; and (2) a physiologically acceptable carrier or excipient. 45. An expression vector comprising the nucleic acid molecule of claim 41 or a fragment thereof, and optionally a promoter operably linked to said nucleic acid molecule or said fragment. 46. A method for recombinantly producing a desired peptide, comprising expressing in a host cell a peptide encoded by the nucleic acid molecule according to claim 41.

Inventors: WIEMANN STEFAN, WIEMANN, STEFAN
Applicants: GERMAN HUMAN GENOME PROJECT, WIEMANN STEFAN, WIEMANN, STEFAN
Application year: 2000
Main classification: C07K14
IPC codes: C07K14, C12N15