6-SUBSTITUTED BIARYL PURINE DERIVATIVES AS POTENT CYCLIN/CDK INHIBITORS AND ANTIPROLIFERATIVE AGENTS

Abstract: The present invention is directed to 2,6,9-trisubstituted purines cellular proliferation. Processes of preparing such compounds are also disclosed.

Description: 6-SUBSTITUTED BIARYL PURINE DERIVATIVES AS POTENT CYCLIN/CDK INHIBITORS AND ANTIPROLIFERATIλ E AGENTS This application claims benefit of U.S. Provisional Patent Application Serial No. 60^124.829. filed March 17. 1999. FIELD OF THE INVENTION The present invention relates to compounds that are shown to be potent cyclin/cyclin dependent kinase (cdk) inhibitors. Compounds with these properties are shown to be potent inhibitors of cell growth and proliferation. Such compounds can be used to treat the following conditions: rheumatoid arthritis, lupus, type 1 diabetes, multiple sclerosis, cancer, restenosis. gout and other proliferative diseases involving abnormal cellular proliferation. Compounds of the present invention which are biaryl substituted purine derivatives are shown to be potent antiproliferatix e agents against a number of human transformed cell lines, and also inhibitors of human cyclin/cdk kinase complexes. BACKGROUND OF THE INVENTION Cellular Proliferation and Cancer. The disruption of external or internal regulation of cellular growth can lead to uncontrolled proliferation and in cancer, tumor formation. This loss of control can occur at many levels and, indeed, does occur at multiple levels in most tumors. Further, although tumor cells can no longer control their own proliferation, they still must use the same basic cellular machinery employed by normal cells to drive their growth and replication. Cvclin Dependent Kinases and Cell Cvcle Retaliation. Progression of the normal cell cycle from the G l to S phase, and from the G2 phase to M phase is dependent on cdks (Sherr, C.J.. Science 274: 1672-1677 (1996)). Like other kinases. cdks regulate molecular events in the cell by facilitating the transfer of the terminal phosphate of adenosine triphosphate (ATP) to a substrate protein. Isolated cdks require association with a second subunit. called cyclins (Desai et al.. Mol. Cell. Biol . 15:345-350 (1995)). Cyclins cause conformational changes at the cdk active site, allowing ATP access and interaction with the substrate protein. The balance between its rates of synthesis and degradation controls the level of each cyclin at any point in the cycle (Elledge. S.J.. et al.. Biochim. Biophvs. Acta. 1377:M61 -M70 ( 1998)). The influences of cyclin/cdk activity on the cell cycle and cellular transformation are summarized in Table 1 . Abnormal Cvclin'cdk Activity in Cancer. In a normal cell, interlocking pathways respond to the cell ' s external environment and internal checkpoints monitor conditions within the cell to control the activity of cvclin 'cdk complexes. A reasonable hypothesis is that the disruption of normal control of cyclin/cdk activity may result in uncontrolled proliferation. This hypothesis appears to hold in a number of tumor types in which cyclins are expressed at elevated levels (Table 1 ). Mutations in the genes encoding negative regulators (proteins) of cyclin/cdk activity are also found in tumors (Larsen, C.-J.. Prog. Cell Cycle Res.. 3: 109-124 (1997)): (Kamb. A.. Trends in Genetics. 1 1 : 136-140 (1995)). Members of the Cip family of cdk inhibitors form a ternary complex with the cyclin/cdk and require binding to cyclinA, cyclinE. or cyclinD (Hall. M.. et al., Oncoaene, 1 1 : 1581 -1588 (1995)). In contrast. Ink family members form a binary complex with cdk4 or cdk6 and prevent binding to cyclinD (Parry. D.; et al., EMBO • , 14:503-51 1 (1995)). - j - Table 1. Associations Among Cyclins and Cancers Inhibitors of Cyclin/cdk Complexes as Potential Anticancer Agents. Tumors with elevated cyclin/cdk activity, whether from the over expression of cyclins or the loss of an endogenous cdk inhibitor, are prime targets for potential therapies based on small molecule cyclin/cdk inhibitors. In fact, several small molecule inhibitors of cyclin/cdks are reported (Meijer. L.. et al., "Progress in Cell Cycle Research." Plenum Press' New York. 351 -363 (1995)) and appear to bind at the ATP site of the kinase. Some information is known about small molecule inhibitors of other kinases, such as PKC (serine kinase) (Murray, K.J. et al.. "Ann. Rep. Med. Chem.. '* J. Bristol. Ed.. Academic Press. Inc.: New York. Chapter 26 (1994)) and tyrosine kinases (Fantl. W.J.. et al.. Ann Re\ . Biochem.. 62:453 (1993 ). Burke. T.R . Prims of the Future. 17 1 19-1 131 ( 1992). Dobrusin. E.M. et al . "Ann. Rep. Med. Chem. " J. Bristol. Ed.. Academic Press. Inc.: New York. Chapter 18 (1992): Spence. P.. Curr. Opin. Ther. Patents. 3:3 ( 1993)). A number of known inhibitors were obtained from commercial sources or were synthesized by literature procedures. Purine Compounds as Cvclin cdk Inhibitors. There are several reports of 2.6-diamino substituted purine derivatives as cyclin/cdk inhibitors and as inhibitors of cellular proliferation. Among those are reports by U.S. Patent No. 5.583.137 to Coe. et al.. olomoucine (Vesely. J.. et al.. Eur. J. Biochem.. 224:771 -786 ( 1994)). roscovitine (Meijer. L.. Eur. J. Biochem.. 243 :527- 536 ( 1997)). WO 97/16452 to Zimmerman. Imbach. P.. et al.. Biooπ-. Med. Chem. Lett.. 9:91 -96 (1999). Norman. T.C.. et al.. J. Amer. Chem. Soc. . 1 18:7430-7431 (1996). Gray. N.S.. et al.. Tetrahedron Lett.. 38: 1 161 -1 164 (1997). Gray. N.S.. et al.. Science. 281 :533-538 (1998). WO 98/05335 to Lum. et al., Schow. S.R.. et al.. Bioor-i. Med. Chem. Lett. 7:2697-2702 ( 1997). US Patent No.. 5.886.702 to Mackman. et al.. Nugiel, D.A.. et al.. J. Oru. Chem.. 62:201-203 (1997). and Fiorini, M.T. et al.. Tetrahedron Lett. 39: 1827-1830 (1998). Many of these reported compounds are shown to inhibit cyclin cdk complexes and have modest cellular proliferation inhibition properties. The compounds of the present invention are shown to have far superior biological activities as cyclin cdk complex inhibitors as well as inhibitors of cellular proliferation compared to those previously reported. In fact, the art (e.g., Fiorini. M.T. et al.. Tetrahedron Lett.. 39: 1827-1830 (1998)) teaches away from compounds of this invention, claiming lack of cellular proliferation inhibition. SUMMARY OF THE INVENTION The compounds of the present invention are 2.6.9-trisubstituted purine derivatives which are inhibitors of cyclin/cdk complexes. The compounds of the current invention also are potent inhibitors of human cellular proliferation. As such, the compounds of the present invention constitute pharmaceutical compositions with a pharmaceutically acceptable carrier. Such compounds are useful in inhibiting cellular proliferation in a mammal b> administering to such mammal an effective amount of the compound. In one embodiment, the compounds of the present i ention are represented by the chemical structure found in Formula I Formula I v. herein: Ri are the same or different and independently selected from: H; Cι-C 4 -straight chain alkyl; CVC 4 -branched chain alkyl; X= N; CH; R 2 = phenyl; substituted phenyl, wherein the substituents (1 -2 in number) are in any position and are independently selected from Ri, OR], SRi, S(O)R|. S(O 2 )Rι, NHRi. NO 2 , OC(O)CH .. NFIC(O)CH 3 , F. Cl. Br. CF 3 , C(O)R,, C(O)NHR,. phenyl, C(O)NHCHR,CH 2 OH; 1 -naphthyl; 2-naphthyl; heterocycles including: 2-pyridyl; 3-pyridyl; 4-pyridyl; 5-pyrimidyl: thiophene-2-yl; thiophene-3-yl; 2-furanyl; 3-furanyl; 2-benzofuranvl; benzothiophene-2-yl: 2-pyrrolyl: 3-pyrrolyl: 2-quinolinyl: 3-quinolinyl: 4-quinolinyl: 1 -isoquinolinyl: 3-isoquinolinyl; 4-isoquinolinyl: substituted heterocvcle. wherein the substituents (1 -2 in number) are in any position and are independently selected from Br. Cl. F. R \ . C(0)CH ; R 3 are the same or different and independently selected from: H; C i -C -straight chain alkyl: CVC-. -branched chain alkyl; CVC -alkenvl chain: (CH 2 ) n Ph: (CH 2 ) n -substituted phenyl. wherein the phenyl substituents are as defined above in R 2 : C|-C 4 -straight chain alkyl: C3-C 4 -branched chain alkyl; R 3 and R can be linked together by a carbon chain to form a 5-8-membered ring; n= 0-3; Y= H; OR,; NHR,; NHC(O)R 3 ; NHC(O)NHR 3 ; NHC(O)R 5 ; NHC(O)OR 6 ; R = C 3 -C7-cycloalkyl; R(,= Cι-C -straight chain alkyl; CVC 4 -branched chain alkyl; C 2 -C -alkenyl chain; (CH 2 ) n Ph; (CH 2 ) n -substituted phenyl. wherein the phenyl substituents are as defined above in R 2 ; or a pharmaceutically acceptable salt thereof. with the and X=CH. then R 3 ≠H. and n≠O. and R ≠H. and Y≠OH. Another aspect of the present invention is directed to a compound of the follow inc formula: Formula III wherein: Ri are the same or different and independently selected from: H; Cι-C -straight chain alkyl; C 3 -C -branched chain alkyl; X= N; CH; R 2 = phenyl; substituted phenyl, wherein the substituents (1 -2 in number) are in an}' position and independently selected from OR], SRi, S(O)R], S(O 2 )Rι, NHRi, NO 2 . OC(O)CH 3 , NHC(O)CH 3 . F, Cl, Br. CF 3 . C(O)R,, C(O)NHR|, phenyl, C(O)NHCHR,CH 2 OH; heterocycles including: 2-pyridyl; 3-pyridyl; 4-pyridyl; 5-pyrimidyl; thiophene-2-yl; thiophene-3-yl; 2-furanyl; -f uranyl; 2-benzofuranyl; benzothiophene-2-yl; 2-pyrrolyl; 3-pyrrolyl; 2-quinolinyl: 3-quinolinyl; 4-quinolinyl: 1 -isoquinolinyl: 3-isoquinolinyh 4-isoquinolinyl: substituted heterocvcle. wherein the substituents ( 1 -2 in number) are in am position and are independently selected from Br. Cl. F. R \ . C(0)CH ; Y- OR, ; NHR,: NHC(O)R, : NHS0 2 Rι; NHC(0)NHRι ; NHC(0)OR (l ; or a pharmaceutically acceptable salt thereof; R(, = Ci-Ci-straight chain alkyl; C3-C -branched chain alkyl; C2-C 4 -alkenyl chain; or a pharmaceutically acceptable salt thereof. The present invention is also directed to a process for preparation of a purine derivative compound of the formula: Formula XVIII wherein: R,= H; Cι-C -straight chain alkyl; C3-C 4 -branched chain alkyl; X- N; CH: R == phenyl; substituted phenyl. wherein the substituents ( 1 -2 in number) are in any position and are independently selected from Ri . OR) . SRi . S(0)Rι . S(0 2 )Rι . NHRi. N0 2 . OC(0)CH .. NHC(O)CH 3 . F. Cl. Br. CF 3 . C(0)R, . C(0)NHR, . phenyl. C(0)NHCHR,CH 2 OH; 1 -naphthyl; 2-naphthyl: heterocycles including: 2-pyridyl; 3-pyridyl; 4-pyridyl; 5-pyrimidyl: thiophene-2-yl; thiophene-3-yl: 2-furanyl; 3-furanyl; 2-benzofuranyl: benzothiophene-2-yl; 2-pyrrolyl; 3-pyrrolyl; 2-quinolinyl; 3-quinolinyl; 4-quinolinyl; 1 -isoquinolinyl; 3-isoquinolinyl: 4-isoquinolinyl; substituted heterocycle. wherein the substituents are in any position and are selected from Br. Cl. F. R, , C(0)CH 3 ; R 3 = H; Cι-C -straight chain alkyl; C 3 -C -branched chain alkyl; C 2 -C -alkenyl chain; (CH 2 ) n Ph; (CFL) n -substituted phenyl. wherein the phenyl substituents are as defined above in R 2 : R-ι= H; C)-C -straight chain alkyl; C 3 -C 4 -branched chain alkyl: R3 and R 4 can be linked together by a carbon chain to form a 5-8-membered ring; n= 0-3; H; OR,; NHC(O)R,: NHC(O)NHR 3 : NHC(O)R .; NHC(0)OR 6 : R^= C .-C . -cvcloalkvl; R(,= Cι-C -straight chain alkyl: C-,-C -branched chain alkyl; C 2 -C -alken> l chain; (CH 2 )nPh: (CH 2 ) n -substituted phenyl. wherein the pheny l substituents are as defined abo\ e in R 2 : or a pharmaceutically acceptable salt thereof with the proviso that when Rι=CH(CH 3 ) 2 , and R 2 =Ph. and X=CH. then R 3 ≠H. and n≠O. and R-^≠H. and Y≠OH. said process comprising: reacting a compound of the formula: I (iπmihi λVI l with a compound of the formula: l or uia \ HI under conditions effective to form the purine derivative compound. Another aspect of the present invention is directed to a process for preparation of a purine derivative compound of the formula: Formula X wherein: Ri are the same or different and independently selected from: H; Cι-C -straight chain alkyl; C 3 -C -branched chain alkyl; X= N; CH; R2= phenyl; substituted phenyl, wherein the substituents (1 -2 in number) are in any position and are independently selected from Ri. ORi, SRi, S(O)R|. S(O 2 )Rι, NHRi. NO 2 , OC(O)CH 3 . NHC(0)CH . F. Cl. Br. CF 3 . C(O)R,. C(0)NHR,. phenyl. C(O)NHCHR,CH 2 OH; 1-naphthyl; 2-naphthyl; heterocycles including: 2-pyridyl; 3-pyridyl; 4-pyridyl; 5-pyrimidyl: thiophene-2-yl: thiophene-3-yl: 2-furanyl; 3-furanyl: 2-benzofuranyl: benzothiophene-2-yl: 2-pyrrolyl; 3-pyrrolyl; 2-quinolinyl; 3-quinolinyl; 4-quinolinyl; 1 -isoquinolinyl; 3-isoquinolinyl: 4-isoquinolinyl: substituted heterocycle. wherein the substituents ( 1 -2 in number) are in any position and are selected from Br. Cl. F. R| . C(0)CH : R 3 are the same or different and independently selected from: H: Cι-C -straight chain alkyl; CVC -branched chain alkyl; C 2 -C -alkenyl chain; (CH 2 ) n Ph; (CH 2 ) n -substituted phenyl. wherein the phenyl substituents are as defined above in R 2 ; R = H: Cι-C -straight chain alkyl; C 3 -C -branched chain alkyl; R 3 and IG, can be linked together by a carbon chain to form a 5-8-membered ring: n= 0-3; Y= H; OR, ; NHR,; NHC(O)R 3 ; NHC(0)NHR 3 ; NHC(O)R > ; NI IC(0)OR 6 ; R. = C 3 -C 7 -cycloalkyl: R (1 = Cι-C -straight chain alky C 3 -C -branched chain alkyl: C 2 -C -alkenvl chain: (CH 2 )„Ph: (CH 2 )„-substituted phen\ l. wherein the phenyl substituents are as defined above in R 2 : or a pharmaceutically acceptable salt thereof. with the proviso that when Ri = CH(CH 3 ) 2 and R 2 = Ph and X = CH. then R 3 ≠ H. and n ≠ O. and R 4 ≠ H. and Y ≠ OH. said process comprising: reacting a compound of the formula: Formula IX wherein Z = Br or with a compound of the formula: R 2 -B(OH)2. R 2 -Sn(n-Bu) . R 2 -Sn(Me) 3 , or mixtures thereof, under conditions effective to form the purine derivative compound. The compounds of the present invention, as described in Formula I. show significantly improved growth inhibition of human transformed cell lines and/or cyclin/cdk inhibition relative to compounds of the prior art. These compounds have been demonstrated to be potent growth inhibitors in dozens of human transformed cell lines. Olomoucine. a structurally related purine derivative, is a poor human transformed cell growth inhibition agent with GLo values in the 20.000-100.000 nM range over 60-transformed cell lines. By contrast, the compounds of the present invention demonstrate GLo values over 60-transformed cell lines in the <10-25.000 nM range, preferably in the < 10-100 nM range o\ er 60-transformed cell lines, and. most preferably. <10 nM across 60-human transformed cell lines. This finding is unexpected from the prior art. which specifically teaches that compounds of the present invention would not be potent human transformed cell line growth inhibitors. The R 2 group in Formula I imparts unexpected and significant improvement in growth inhibition in human transformed cell lines, while substitution of various groups at R 3 and R 4 found in Formula I impart important features that contribute to cyclin cdk inhibition and growth inhibition of human transformed cell lines. Specifically, the combination of the R 2 group and the substitutions within R and R- 4 result in compounds with superior biological activity. Compounds which are cyclin/cdk inhibitors and/or human transformed cell line growth inhibitors have utility in treating human proliferative cellular disorders. DETAILED DESCRIPTION OF THE INVENTION The compounds of the present invention are represented by the chemical structure found in Formula II. Formula II wherein: Ri are the same or different and independently selected from: H; Cι-C -straight chain alkyl; C 3 -C -branched chain alkyl; X= N: CH; R 2 = phenyl; substituted phenyl. wherein the substituents (1 -2 in number) are in any position and independently selected from R t . ORj. SRi . S(O)R| . S(0 2 )R, . NHRi. N0 2 . OC(O)CH 3 . NHC(O)CH 3 . F. Cl. Br. CF ,. C(0)R, . C(O)NHR| . phenyl. C(0)NHCHR,CH 2 OH; 1 -naphthyl; 2-naphthyl: heterocycles including: 2-pyridyl; 3-pyridyl: 4-pyridyl; 5-pyrimidyl: thiophene-2-yl; thiophene-3-yl; 2-furany ; 3 -f uranyl; 2-benzofuranyl; benzothiophene-2-yl : 2-pyrrolyl; 3-pyrrolyl; 2-quinolinyl; 3-quinolinyl; 4-quinolinyl: 1 -isoquinolinyl; 3-isoquinolinyl; 4-isoquinolinyl; substituted heterocycle. wherein the substituents (1-2 in number) are in any position and are independently selected from E3r. Cl. F. R| . C(0)CH 3 ; R 3 are the same or different and independently selected from: H; Cι-C -straight chain alkyl; C 3 -C -branched chain alkyl; Cι-C -straight chain alkyl; C 3 -C -branched chain alkyl; R 3 and R can be linked together by a carbon chain to form a 5-8-membered ring; n= 0-3; Y= H; OR, ; NHR, NHC(0)R 3 : NHC(O)NHR 3 : or a pharmaceutically acceptable salt thereof: with the proviso that w hen R,=CH(CFl 3 ) . and R 2 =Ph. and X=CH. then R ≠H. and n≠O. and R_,≠H. and Y≠OH. More preferably, the compounds of the current invention are represented by the chemical structure found in Formula III . Formula III wherein: Ri are the same or different and independently selected from: H; C ι-C -straight chain alkyl; C ,-C -branched chain alkyl; X= N; CH; phenyl; substituted phenyl. wherein the substituents (1 -2 in number) are in any position and independently selected from R], OR,. SRi . S(O)Rι , S(O 2 )R|. NHRi . N0 2 . OC(O)CH ., NHC(O)CH 3 . F, Cl. Br, CF 3 , C(O)R, . C(0)NHR, . phenyl, C(O)NHCHRιCH 2 OH; heterocycles including: 2-pyridy : 3-pyridyl; 4-pyridyl; 5-pyrimidyl: thiophene-2-yl; thiophene-3-yl; 2-furanyl; 3-furanvl: 2-benzofuranyl: benzothiophene-2-yl: 2-pyrrolyl: 3-pyrrolyl: 2-quinolinyl: 3-quinolinyl: 4-quinolinyl: 1 -isoquinolinyl: 3-isoquinolinyl: 4-isoquinolinyl; substituted heterocycle. wherein the substituents (1 -2 in number) are in any position and are independently selected from Br. Cl. F. R|. C(0)CH 3 ; Y= OR,; NHC(0)R, : NHS0 2 Rι; HC(0)NHR,: NHC(0)OR : or a pharmaceutically acceptable salt thereof: R(, = C,-C -straight chain alkyl; C 3 -C -branched chain alkyl; C 2 -C -alkenyl chain; or a pharmaceutically acceptable salt thereof. In another embodiment, the present invention is directed to a method for inhibiting cellular proliferation in mammals comprising administering a therapeutically effective amount of the compound of the present invention to the mammal. The compounds of the present invention can be administered orally, parenterally, for example, subcutaneously, intravenously, intramuscularly, intraperitoneally, by intranasal instillation, or by application to mucous membranes, such as, that of the nose, throat, and bronchial tubes. They may be administered alone or with suitable pharmaceutical carriers, and can be in solid or liquid form such as, tablets, capsules, powders, solutions, suspensions, or emulsions. Based on the results obtained in the standard pharmacological test procedures described below, the compounds of the present invention are useful as antineoplastic agents. More particularly, the compounds of the present invention are useful for inhibiting the growth of neoplastic cells, causing cell death of neoplastic cells, and eradicating neoplastic cells. The compounds of the present invention are. therefore, useful for treatinsz solid tumors. including sarcomas and carcinomas, such as astrocytomas. prostate cancer, breast cancer, small cell lung cancer, and ovarian cancer, leukemias. Ivmphomas. adult T-cell leukemia/h'mphoma. and other neoplastic disease states. In addition to the utilities described above, many of the compounds of the present invention are useful in the preparation of other compounds. The active compounds of the present invention may be orally administered, for example, with an inert diluent, or with an assimilable edible carrier, or the}' may be enclosed in hard or soft shell capsules, or they may be compressed into tablets, or they may be incorporated directly with the food of the diet. For oral therapeutic administration, these active compounds ma}' be incorporated with excipients and used in the form of tablets, capsules, elixirs, suspensions, syrups, and the like. Such compositions and preparations should contain at least 0.1 % of active compound. The percentage of the compound in these compositions may. of course, be varied and may conveniently be between about 2% to about 60% of the weight of the unit. The amount of active compound in such therapeutically useful compositions is such that a suitable dosage will be obtained. Preferred compositions according to the present invention are prepared so that an oral dosage unit contains between about 1 and 250 mg of active compound. The tablets, capsules, and the like may also contain a binder such as gum tragacanth. acacia, corn starch, or gelatin: excipients such as dicalcium phosphate; a disintegrating agent such as corn starch, potato starch, alginic acid; a lubricant such as magnesium stearate; and a sweetening agent such as sucrose, lactose, or saccharin. When the dosage unit form is a capsule, it may contain, in addition to materials of the above type, a liquid carrier such as a fatty oil. Various other materials may be present as coatings or to modify the physical form of the dosage unit. For instance, tablets may be coated with shellac, sugar, or both. A syrup may contain, in addition to active ingredient, sucrose as a sweetening agent, methyl and propylparabens as preservatives, a dye. and flavoring such as cherry or orange flavor. These active compounds may also be administered parenterally. Solutions or suspensions of these active compounds can be prepared in water suitably mixed with a surfactant such as hydroxypropylcellulose. Dispersions can also be prepared in gh cerol. liquid polyethylene glycols. and mixtures thereof in oils. Illustrative oils are those of petroleum, animal, vegetable, or synthetic origin, for example, peanut oil. soybean oil. or mineral oil. In general, water, saline, aqueous dextrose and related sugar solution, and glycols such as. propylene glycofor polyethylene glycol. are preferred liquid carriers, particularly for injectable solutions. Under ordinary conditions of storage and use. these preparations contain a preservative to prevent the growth of microorganisms. The pharmaceutical forms suitable for injectable use include sterile aqueous solutions or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersions. In all cases, the form must be sterile and must be fluid to the extent that easy syringability exists. It must be stable under the conditions of manufacture and storage and must be preserved against the contaminating action of microorganisms, such as bacteria and fungi. The carrier can be a solvent or dispersion medium containing, for example, water, ethanol. polyol (e.g.. glycerol. propylene glycol. and liquid polyethylene glycol). suitable mixtures thereof, and vegetable oils. The compounds of the present invention may also be administered directly to the airways in the form of an aerosol. For use as aerosols, the compounds of the present invention in solution or suspension may be packaged in a pressurized aerosol container together with suitable propellants. for example, hydrocarbon propellants like propane, butane, or isobutane with conventional adjuvants. The materials of the present invention also may be administered in a non-pressurized form such as in a nebulizer or atomizer. General Synthetic Schemes The compounds of the present invention can be prepared by conventional methods of organic synthesis practiced by those skilled in the art. The general reaction sequences outlined below are general methods useful for preparing the compounds of the present invention and are not meant to be limiting in scope or utility. Reaction of 2.6-dichloropurine (Formula IV) with various amines of Formula V in the presence of a polar solvent, such as ethanol. provides purines of Formula VI (General Flowsheet I. infra). Reaction of purines of Formula VI with alkyl halides (Ri-Z) in the presence of a base such as potassium carbonate provides N l -alkylated purines of Formula VII. Chloride displacement with \-alkylated purines of Formula VII with amines of structure Formula VIII in an inert solvent such as ethanol or butanol at an appropriate temperature provides purines of Formula IX. Transition metal-mediated cross-coupling reaction of purines of Formula IX w ith boronic acid (R 2 -B(OFI) 2 ) or tin reagents (R 2 -Sn(n-Bu) or R -SnMe 3 ) provides purines of Formula X. If in Formula X (Y=NH 2 ). then subsequent reaction of Formula X (Y=NH 2 ) with acid chloride (R 3 COC I ). or sulfonyl chloride (R 3 S0 2 C 1 ). or isocyanate (R 3 NCO). or chloroformate (C l C(0)OR ) reagents provides purines of Formula XI wherein Y=NHC(0)R 3 . NHS0 2 R . or NHC(0)NHR 3 . or NHC(0)OR 6 , respectivch . On the other hand, if in Formula X. Y already is OR, or NHC(0)R 3 or NHSO 2 R3 or NHC(0)NHR 3 or NHC(O)OR . as a result of what Y started out as in Formula VIII, then this last step is unnecessary. Definitions of the groups include: Ri are the same or different and independently selected from: H; C,-C -straight chain alkyl; C;,-C -branched chain alkyl; Z= Br; R 2 = phenyl; substituted phenyl, wherein the substituents (1 -2 in number) are in any position and independently selected from R,, ORi, SRi . S(O)Rι, S(O 2 )Rι , NHRi . NO 2 , OC(O)CH 3 . NHC(O)CH 3 . F, Cl. Br. CF 3 . C(O)R,, C(O)NHRι , phenyl. C(0)NHCHR,CH 2 OH; 1 -naphthyl; 2-naphthyl; heterocycles including: 2-pyridyl; 3-pyridyl; 4-pyridyl; 5-pyrimidyl; thiophene-2-yl; thiophene-3-yl; 2-furanyl; 3-furanvl: 2-benzof uranyl; benzothiophene-2-yl; 2-pyrrolyl: 3-pyrrolyl: 2-quinolinyl; 3-quinolinyl: 4-quinolinyl; 1 -isoquinolinyl; 3-isoquinolinyl; 4-isoquinolinyl; substituted heterocycle. wherein the substituents (1 -2 in number) are in any position and are independently selected from Br. Cl. F. R,. C(0)CH 3 : R 3 are the same or different and independently selected from: H; C,-C -straight chain alkyl: C 3 -C -branched chain alkyl: C 2 -C -alkenvl chain; (CH 2 ) Ph; (CFL)„-substituted phenyl. wherein the phenyl substituents are as defined above in R 2 : R = FI; C,-C -straight chain alkyl: C 3 -C -branched chain alkyl: R 3 and R 4 can be linked together by a carbon chain to form a 5-8-membered ring: n= 0-3; Y= H; OR,; NHR, ; NHC(O)R 3 ; NHC(0)NHR 3 ; NHC(O)OR 6 ; R = C,-C -straight chain alkyl; C 3 -C -branched chain alkyl; C 3 -C 7 -cycloalkyl; C 2 -C -alkenyl chain; (CH 2 )„Ph; (CH 2 ) n -substituted phenyl. wherein the phenyl substituents are as defined above in R?. General Flowsheet I Formula VI R,-Z:basc Formula Nil Formula IX R B(011) 2 or R Sn(n-Bu), or R Sn(Mc) . .: , Pi(ϋ) catalyst Formula X Formula XI General non-limiting syntheses of compounds of the present invention of Formula XVIII and Formula XIX are shown below. General Flowsheet II R B(011) : or Formula XII Formula XIII Formula XI\' Reduce Sohent Formula IV Solvent Formula XVII Formula XVI Formula XV Formula XVIII Formula XIX Reaction of acids of Formula XII with oxah l chloride or thiom l chloride followed by reaction with ammonium hydroxide provides amides of Formula XIII (General Flowsheet II). Transition metal-mediated cross-coupling reaction of amides of Formula XIII w ith boronic acid (R 2 -B(OH) 2 ) or tin reagents (R 2 -Sn(n-Bu) 3 ) or (R 2 - SnMe^) provides amides of Formula XIV. Reduction of amides of Formula XIV with a reducing agent in an appropriate solvent provides amines of Formula XV. Reaction of amines of Formula XV with 2.6-dichloropurine (Formula IV) in the presence of a polar solvent, such as ethanol, provides purines of Formula XVI. Reaction of purines of Formula XVI with alkyl halides (Ri-Z) in the presence of a base such as potassium carbonate provides N l -alkylated purines of Formula XVII. Chloride displacement of purines of Formula XVII w ith amines of Formula VIII in an inert solvent such as ethanol or butanol at an appropriate temperature provides purines of Formula XVIII. If in Formula XVIII (Y=NH 2 ). then subsequent reaction of Formula XVIII (Y=NFL) with acid chloride R 3 COC I ). or sulfonyl chloride (R 3 SO 2 C 1 ), or isocyanate (R3NCO). or chloroformate (C 1 C(0)OR 6 ) reagents provides purines of Formula XIX wherein Y=NHC(O)R 3 . or NFISO 2 R . 3, or NHC(O)NHR 3 . or NHC(O)OR 6 . respectively. On the other hand, if in Formula XVIII. Y already is OR, or NHC(O)R 3 or NHSO 2 R 3 or NHC(0)NHR 3 or NHC(O)OR 6 , as a result of what Y started out as in Formula VIII. then this last step is unnecessary. Definitions of the groups include: Ri are the same or different and independently selected from: H; Cι-C -straight chain alkyl; C 3 -C -branched chain alkyl; X= N; CH; Z= Br; I; R 2 = phenyl; substituted phenyl. wherein the substituents ( 1 -2 in number) are in any position and independently selected from R| . OR| . SRi . S(0)Rι, S(O 2 )Rι . NHRi . N0 2 . OC(0)CH ,. NHC(O)CH 3 . F. Cl. Br. CF . C(0)R, . C(0)NHR,. phenyl. C(0)NHCHR,CH 2 OH; 1 -naphthyl; 2-naphthyl; heterocycles including: 2-pyridyl: 3-pyridyl; 4-pyridyl; 5-pyrimidyl; thiophene-2-yl: thiophene-3-yl; 2-furanyl; 3 -f uranyl; 2-benzofuranyl; benzothiophene-2-yl; 2-pyrrolyl; 3-pyrrolyl; 2-quinolinyl; 3-quinolinyl; 4-quinolinyl; 1 -isoquinolinyl: 3-isoquinolinyl; 4-isoquinolinyl; substituted heterocycle. wherein the substituents (1 -2 in number) are in any position and are independently selected from Br. Cl. F. R, . C(O)CH 3 ; R 3 are the same or different and independently selected from: H; C ,-C -straight chain alkyl; C 3 -C -branched chain alkyl; C 2 -C -alkenvl chain; (CH 2 )„Ph; (CFI )„-substituted phenyl. wherein the phenyl substituents are as defined above in R 2 ; C, -C -straight chain alkyl; C 3 -C -branched chain alkyl; R 3 and R can be linked together by a carbon chain to form a 5-8-membered ring; n= 0-3; Y= H; OR, ; NHC(O)R 3 ; NHC(0)NHR 3 : NHC(0)OR 6 : R(,= C ι-C 4 -straight chain alkyl: C ,-C -branched chain alkyl; C ,-C7-cycloalkyl: CVCi-alkenvl chain; (CH 2 ) n Ph. (CFLJ n -substituted phenyl. wherein the phenyl substituents are as defined above in RT . The synthesis of compound 5 is shown below in Scheme I. Scheme I DMSO Pd(PPh 4 Na 2 CO, toluene, water The synthesis of compound 11 is shown below in Scheme II. Scheme II NH OH BH THF THF NH, The syntheses of compounds 12.13 and 14 are shown below in Scheme III. Scheme III Nil. NIF The s} nthesis of compound 17 is shown below in Scheme IV. Scheme IV 15 16 4. Pd(PPh,) 4 toluene The synthesis of compound 17 is shown below in Scheme V. Scheme V 15 K-.C0 3 DMSO The synthesis of compound 25 is shown below in Scheme VI. Scheme VI synthesis of compound 25 is shown below in Scheme VII. Scheme VII EtOH The synthesis of compound 32 is shown below in Scheme VIII. Scheme VIII 31 The syntheses of compounds 33 and 34 are shown below in Scheme IX. Scheme IX Os0 pyr toluene The syntheses of compounds 36. 38. and 40 are shown below in Scheme X. Scheme X The synthesis of compound 43 is shown below in Scheme XI. Scheme XI 43 The synthesis of compound 46 is shown below in Scheme XII. Scheme XII 45 44 in HC1 46 The s\ ntheses of compound 48 and 50 are shown below in Scheme XIII. Scheme XIII The synthesis of compound 53 is shown below in Scheme XIV. Scheme XIV 52 PhB(OH), Pd(PPh 3 ) 4 " Na,C0 3 DME " H,0 53 The synthesis of compound 54 is shown below in Scheme XV. Scheme XV OH The synthesis of compound 56 is shown below in Scheme XVI. Scheme XVI Pd(PPh 3 ) 4 . toluene The synthesis of compound 58 is shown below in Scheme XVII. Scheme XVII PhB(OH), Pd(PPh 3 )4 Na,C0 3 DME H-,0 The synthesis of compound 60 is shown below in Scheme XVIII. Scheme XVIII PhB(OH) 2 Pd(PPh 3 ) 4 Na 2 C0 3 DME H,0 The syntheses of compounds 61. and 62 are shown below in Scheme XIX. Scheme XIX 62 55161 - 47 The s\ ntheses of compounds 64. and 65 are shown below in Scheme XX. Scheme XX HN. " S0 2 CH 3 65 0/55161 - 48 The syntheses of compounds 66. and 67 are shown below in Scheme XXI. Scheme XXI 66 0/55161 -49 The s tthesis of compound 73 is shown below in Scheme XXII. Scheme XXII 73 The syntheses of compounds 74. 75. and 76 are shown below in Scheme XXIII. Scheme XXIII NH, ΛcCI. DMΛP NFt,. CH,C1, The nthesis of compound 77 is shown below in Scheme XXIV. Scheme XXIV OH The synthesis of compound 78 is shown below in Scheme XXV. Scheme XXV NH, An alternative synthesis of compound 78. and the synthesis of compound 79 are shown below in Scheme XXVI. Scheme XXVI NH, Ac,0. DMAP pyr. CH,C1, 33 The s\nthesis of compound 80 is sho n below in Scheme XXVII. Scheme XXVII 80 The syntheses of compounds 86. and 87 are shown below in Scheme XXVIII. Scheme XXVIII 84 κ,co 3 DMSO 3 - The synthesis of compound 88 is shown below in Scheme XXIX. Scheme XXIX 88 The syntheses of compounds 93. and 94 are shown below in Scheme XXX. Scheme XXX LtOI I Act) DMΛP P> r circr UN . .CH T 94 The syntheses of compounds 95. and 96 are shown below in Scheme XXXI. Scheme XXXI Ac,0 pyr. DMAP CH,C1, HN. .CH , T " % O The synthesis of compound 97 is shown below in Scheme XXXII. Scheme XXXII HN. .CH 3 T O 97 The syntheses of compounds 98. and 99 are shown below in Scheme XXXIIl. Scheme XXXIIl NH, The synthesis of compound 100 is shown below in Scheme XXXIV. Scheme XXXIV The syntheses of compounds 101. and 102 are shown below in Scheme XXXV. Scheme XXXV NH, 101 Ac,0, p\r DMAP.CH-.CI HN. H 3 Y O 102 The syntheses of compounds 103. and 104 are shown below in Scheme XXXλ ' l. Scheme XXXVI Ac,0, pyr DMAP. CH,C1, The syntheses of compounds 106. 107. and 108 are shown below in Scheme XXXVII. Scheme XXXVII 106 HC1. Et,0 MeOH, CH,Cl, 108 107 The syntheses of compounds 109. and 110 are shown below in Scheme XXXVIII. Scheme XXXVIII NH, Ac,0 pyr. DMAP CH,C1, The syntheses of compounds 111. and 112 are shown below in Scheme XXXIX. Scheme XXXIX NH, Ac,0 DMAP. pyr CH,C1, The synthesis of compound 113 is shown below in Scheme XL. Scheme XL The syntheses of compounds 114.115.116, and 117 are shown below in Scheme XLI. HN. H 3 NH. .CH 3 NH, T O T O The synthesis of compound 118 is shown below in Scheme XLII. Scheme XLII NH, The syntheses of compounds 123 and 124 are shown below in Scheme XLIII. Scheme XLIII EXAMPLES Proton NMR spectra were obtained on a Bruker AC 300 spectrometer at 300 MHz or a Bruker 500 MHz spectrometer and were referenced to tetramethylsilane as an internal standard. The IR spectrometer used was a single beam Perkin-Elmer Spectrum 1000 FT-IR. All IR spectra obtained were prepared in a pressed disc of KBr. All IR spectra obtained were acquired with a total of 4 accumulations at a resolution of 4.00 cm " 1 . Melting points were obtained on a Mel- Temp II apparatus and are uncorrected. Mass spectra ere obtained on either a Shimadzu QP-5000 or a PE Sciex API 150 Mass Spectrometer. Example 1 - Preparation of Compound 2 To the starting material 1 (1.0 g. 5.29 mmol) was added 4- bromobenzylamine (2.53 g, 1 1 .4 mmol). and EtOH ( 1 1 mL). The mixture was stirred and heated at 50 °C in a round-bottomed flask and then H 2 0 ( 1 mL) and EtOH ( 10 mL) were added to dissolve the solids. The mixture was refluxed for 1 h. Hϋnig ' s base (3.68 mL. 21.2 mmol) was added and refluxed overnight, during which time a precipitate formed. The solution was filtered to provide a light yellow solid. The solid was dried in vacua (1.08 g. 60%): Η NMR (300 MHz. DMSO-J 6 ) δ 8.75 (bs. 1 H). 8.15 (s. 1 H). 7.52 (d. 2 H). 7.30 (d. 2 H). 4.63 (bs. 2 H); Cl MS m/z = 340 LC, 2 Hc,BrClN 5 +H] + . Example 2 - Preparation of Compound 3 To the starting material 2 (1.08 g, 3.19 mmol) was added DMSO (1 1 mL). K CO (2.20 g, 15.95 mmol). and 2-iodopropane (1 mL, 9.57 mmol). The solution was stirred overnight then poured into H 2 0 (75 mL) and stirred. Additional FLO (25-50 mL) was added to the mixture to form a yellow solid. The stirring was continued at 0 °C. The solid was filtered in vacuo. The crude product was purified by silica gel chromatography to provide 3 (0.66 g. 50%) as a white solid: mp 136-140 °C; Η NMR (300 MHz, CDC1 3 ) δ 7.78 (s. 1 I I). 7.49 (d. 2 H). 7.28 (d. 2 H). 6.12 (bs. 1 H). 4.90-4.70 (m. 3 H). 1.61 (d. 6 H). Example 3 - Preparation of Compound 4 To starting material 3 ( 1.44 g. 3.78 mmol) was added 2-amino- 1- butanol (5.06 g. 56.7 mmol) and ethanol (5 mL) and the mixture was heated in a sealed tube in an oil bath at 150-160 °C for 48 h. The cooled solution was transferred to a round-bottomed flask and the ethanol was removed in vacua. The crude product was purified by flash column chromatography on silica gel to give 4 (0.90 g. 55%): Η NMR (300 MHz. CDCh) δ 7.44-7.41 (m. 3 H). 7.23 (d. 2 H). 6.22 (s. 1 H). 5.06 (s. 1 H). 4.90 (d. 1 H). 4.78-4.68 (m. 2 H). 4.65-4.55 (m. 1 H). 3.91 -3.80 (m. 2 H). 3.66- 3.60 (m. 1 H). 1.66-1.47 (m. 8 H). 1.04-0.99 (t. 3 H). Example 4 - Preparation of Compound 5 To starting material 4 (0.13 g, 0.29 mmol) was added 3- acetamidophenylboronic acid (0.21 g. 1.19 mmol) and Pd(PPh 3 ) (0.08 g. 0.07 mmol). Na 2 C0 3 (2M. 0.60 mL). and toluene (5 mL). The solution was degassed with argon for 10 min then heated at 130 °C for 6 h. The cooled solution was diluted with water and then extracted with CFLCL (3 x 50 mL). The combined organic phases were washed with brine, dried over anhydrous Na 2 S0 . filtered, and concentrated to yield a viscous orange oil. The oil was purified by flash column chromatography on silica gel and then the product crystallized upon standing to give 5 (0.06 g. 41%) as a pale yellow solid: Η NMR (300 MHz. CDC1 3 ) δ 8.01-7.21 (m. 9 H). 6.48 (s. 1 H). 4.97 (d. 1 H), 4.82-4.70 (m. 2 H), 4.65-4.53 (m, 1 H). 3.98-3.25 (m, 2 H). 3.20-3.05 (m. 1 H). 2.20 (s. 3 H). 1.69-1.45 (m, 8 H). 1.07-0.98 (t. 3 H). Example 5 - Preparation of Compound 7 To 4-iodobenzoic acid (52.2 g. 0.21 mol) was added CH^CL (500 mL) and DMF (2 drops) at room temperature. Oxalyl chloride (32 g. 0.25 mol) was added dropwise in 0.5 h and stirred for 2 d. The volatiles were removed in vacuo to a volume of 150 mL to give the acid chloride and CFLCL. To a mixture of ice (500 mL) and NH 4 OH (29%: 100 mL) was added the CFLCL solution during 15 min. The resultinu solids were collected, washed with CILCL. and dried in vacuo. The solids were slurried in H 2 0 for 1 h. The solids were collected by filtration, washed in water and acetone, and dried in vacuo to give 7 (48 g: 92%): mp 213-216 °C. Example 6 - Preparation of Compound 8 To a suspension of 7 ( 1 1 g. 45 mmol) in THF (50 mL) was added BH 3 - THF ( I M. 22.5 mL. 22.5 mmol). The resulting solution was heated under reflux overnight. The reaction was cooled in an ice bath and MeOFl-FICl (60 mL) was slowly added dropwise. The resulting precipitate was filtered and dried to give 8 (10.8 g. 88%) as a white solid: mp 256-262 °C dec: Η NMR (300 MHz. DMSO-J 6 ) δ 8.55 (bs. 3 FI). 7.79 (d. 2 H). 7.32 (d. 2 FI). 3.98 (s. 2 H). Example 7 - Preparation of Compound 9 To compound 1 (7.63 g. 40.4 mmol) was added compound 8 (10.8 g. 40.4 mmol). water (123 mL), and Hunig ' s base (14 mL. 81 mmol). The mixture was heated to reflux for 5 h and stirred overnight at room temperature to give a pale yellow solution. An additional quantity of water (150 mL) was added, refluxed for 3 h, then cooled overnight. A pale yellow solid was formed which was filtered, washed with water, rinsed with EtOFI (2 x). and dried in vacuo to give yield 9 (13.3 g. 80%): Η NMR (300 MHz. DMSO-J 6 ) δ 8.68 (bs, 1 H). 8.28 (s. 1 FI). 7.68 (d. 2 FI). 7.50 (d, 2 FI). 5.08 (bs. 1 FI). 4.50 (d. 2 FI). Example 8 - Preparation of Compound 10 To compound 9 (12.2 g. 31.7 mmol) was added K 2 CO 3 (35 g. 0.25 mol). 2-iodopropane (13 g. 0.13 mol) and DMSO (210 mL). The reaction mixture was stirred under N 2 at room temperature overnight, then poured into FLO (1.5 L) and stirred for 2 d. The precipitate was collected as an off-white solid and washed with Et 2 0. The aqueous layer was extracted with EtOAc (2 x) and the combined organic phases were washed with brine, dried over Na 2 S0 . filtered, and evaporated to give an off-white foam (6.4 g). This off-white foam was combined with the precipitate and washed with Et 2 0 to give 10 (1 1.0 g): Η NMR (300 MFIz. DMSO-J 6 ) δ 8.91 (m. 1 H). 8.38 (s. 1 H). 7.74 (d. 2 H). 7.21 (d. 2 H). 5.1 1 (bs. 1 H). 4.68 (m. 1 H). 4.60 (d. 2 H). 1 .48 (d. 6 FI). Example 9 - Preparation of Compound 11 Compound 10 (1.52 g. 3.55 mmol). .ra.7.v-1.4-diaminocyclohexane (6.35 g. 55.60 mmol). and EtOFI ( 18 mL) were placed in a sealed tube. The reaction mixture was heated at 120-190 °C for 24 h. The reaction was then allowed to cool to room temperature. The reaction mixture was filtered and the filtrate evaporated. The residue was purified by column chromatography. and dried in vacuo for 16 h to yield 11 ( 1 .60 g. 89%) as a yellow sticky oil: Η NMR (300 MHz. CDCh) δ 7.62 (d. 2 H). 7.44 (s. 1 FI). 7.08 (d. 2 H). 6.14 (br. 1 H). 4.75-4.63 (m. 2 H). 4.63-4.54 (m. 2 H). 3.75-3.63 (m. 1 H). 2.72-2.57 (m. 2 PI). 2.1 8-2.00 (m. 2 H). 2.00-1 .75 (m. 4 H). 1.54 (d. 6 FI). 1.39-1.00 (m, 3 H); API MS m/z = 506 [C2iH 2 8lN 7 +H] + . Example 10 - Preparation of Compound 12 To compound 11 (0.133 g, 0.26 mmol) was added DME (2.5 mL) and 3-thiopheneboronic acid (0.12 g, 0.97 mmol) in a round-bottomed flask and equipped with a condenser purged with argon. To this was added DME (3 mL) followed by tris(dibenzylidoneacetone)dipalladium (0.01 g, 0.01 mmol) and PPh (0.04 g, 0.15 mmol ). Na 2 Cθ 3 (2M, 0.6 mL) and DME ( 1 mL) was added to the reaction mixture and the reaction mixture was allowed to reflux for 18.5 h. then stirred at room temperature under argon for 46 h. The reaction mixture was diluted with H 2 O and extracted with CH 2 CL. The combined organic phases were washed with brine, dried over anhydrous Na 2 SO . filtered, and concentrated in vacuo. The residue was purified by column chromatography to yield 12 (0.050 g, 41 %) as a tan solid: Η NMR (300 MHz. CDCI 3 ) δ 7.56-7.50 (m. 4 H), 7.44-7.35 (m. 3 FI). 6.02 (br, 1 H). 4.78 (d. 2 H). 4.69-4.54 (m, 2 H). 3.75 (br. 1 FI), 2.69 (br. 1 H). 2.15 (br. 2 H). 1.88 (br. 3 FI), 1.54 (d, 7 FI). 1.33-0.97 (m. 4 H); API MS m/z = 462 [C25H31N7S+F-P Example 1 1 - Preparation of Compound 13 DME (3 mL). tris(dibenzylidoneacetone)dipalladium (0.01 g. 0.01 mmol). and PPh 3 (0.04 g. 0.15 mmol) were placed in a round-bottomed flask fitted with a condenser and maintained under argon. Compound 11 (0.13 g. 0.26 mmol). and 4-methylbenzeneboronic acid (0.13 g. 0.98 mmol) dissolved in Na Cϋ 3 (2M. 0.6 mL) and DME ( 1 mL) were added to the reaction mixture. The reaction mixture was refluxed for 19.5 h and stirred at room temperature for 4 h. The reaction mixture was diluted with water and extracted with CHiCL. The combined organic phases were washed with brine, dried over anhydrous Na 2 S0 . and evaporated. The crude product was purified by column chromatography and dried in vacuo for 22 h to yield the desired product 13 (54 mg. 44%) as an off-white solid: Η NMR (300 MHz. CDC1 . .) δ 7.56-7.41 (m. 7 H). 7.23 (s. 1 H). 5.92 (br. 1 H). 4.83 (d, 2 H). 4.74-4.58 (m. 2 H). 3.77 (br. 1 H). 2.70 (br. 1 H). 2.40 (s. 3 H). 2.16 (d. 3 I I). 1.88 (d. 3 H). 1.55 (d. 7 H). 1.33-0.97 (m. 4 FI); API MS m/z = 470 [C^I^ . Nv+Hf. Example 12 - Preparation of Compound 14 DME (3 mL). tris(dibenzylideneacetone)dipalladium (0.01 g. 0.01 mmol). and PPh- 3 (0.04 g, 0.15 mmol) were placed in a round-bottomed flask with a condenser under argon. Compound 11 (0.13 g. 0.25 mmol) and 3-chloro-4- fluoroboronic acid (0.15 g. 0.88 mmol) were dissolved in Na 2 C0 3 (2M. 0.6 mL) and DME ( 1 mL) were added to the reaction mixture, refluxed for 19 h then stirred at room temperature for 2 h. The reaction mixture was diluted with water and extracted with CFLCF. The combined organic phases were washed with brine, dried over anhydrous Na 2 S0 . and evaporated. The crude product was purified by repeated column chromatography to yield 14 (0.019 g. 15%): Η NMR (300 MHz. CDC1 3 ) δ 7.59-7.53 (m. 1 PI), 7.47-7.35 (m. 4 FI), 7.26-7.14 (m. 3 H). 5.81 (br. 1 FI). 4.81 (d. 2 H), 4.72-4.54 (m, 2 H). 3.72 (br, 1 FI), 2.69 (br. 1 H), 2.21 -2.03 (m. 3 H). 1.94-1.78 (m. 3 H). 1.54 (d. 6 FI). 1.33-1.12 (m. 4 H): API MS m/z = 508 [C 27 H 3 |C1FN 7 +Hf. Example 13 - Preparation of Compound 16 A solution of 15 (2.5 g. 15.8 mmol) and ether was cooled to -78 °C. In a separate flask. π-BuLi (15.8 mmol) was also cooled to -78 °C. The solution of 15 was added to the π-BuLi solution via cannula to give a dark red solution. The reaction mixture was stirred for 5 min prior to the rapid addition of (/7-Bu) 3 SnCl (6.2 g. 19 mmol). The resulting bright yellow solution was stirred at -78 °C for 2 h. allowed to warm to room temperature, and stirred for another 10 min. The solution was then diluted with H 2 0 (80 mL) and extracted with ethyl acetate (3 x 50 mL). The organic extracts were combined, ashed with brine, dried over Na 2 S0 . filtered, and concentrated in vacuo to yield the crude product as a yellow oil. Purification by column chromatography gave the product 16 (4.89 g. 84%) as a pale yellow liquid: Η NMR (300 MHz. CDCL) δ 8.72 (d. 1 H). 7.48-7.46 (m. 1 H). 7.40-7.38 (m. 1 FI). 7.1 1-7.09 (m, 1 H). 1.61-1.50 (m. 6 H), 1.38-1.26 (m, 6 H). 1.14-1.09 (m. 6 H). 0.97- 0.77 (t. 9 H). Example 14 - Preparation of Compound 17 To compound 16 (0.18 g, 0.48 mmol) was added compound 4 (0.14 g, 0.33 mmol). Pd(PPh ) (0.05 g, 0.49 mmol). and toluene (10 mL) in a sealed tube under an argon atmosphere. The solution was degassed with argon and heated at 135 °C in an oil bath for 3 h. The solution was cooled to room temperature, diluted with saturated NaHCO;,. and extracted ith CH2CI2 (3 x 30 mL). The organic layer was washed with brine, dried over Na 2 S0 , and concentrated in vacuo to give a light brown oil. The residue was purified by flash column chromatography using MeOH/C^CL (10%>) to afford 17 as a white solid. The sample was dissolved into hexane/CF CL/MeOH and then precipitated with diethyl ether, filtered, and rinsed several times with ether to provide in 17 (30.3 mg): mp 95-100 °C; 'FI NMR (300 MHz. CDCI 3 ) δ 8.68 (d. 1 H). 7.96 (d. 2 H). 7.77-7.69 (m, 2 FI). 7.49-7.45 (m. 3 H), 7.24-7.20 (m. 1 H). 5.99 (s. 1 FI), 5.1 1 (s. 1 H). 4.88-4.83 (m, 3 H). 4.65-4.56 (m. 1 FI). 3.91-3.80 (m. 2 FI). 3.65-3.60 (m. 1 FI). 1.66-1.52 (m, 8 FI), 1.05-0.99 (t. 3 H): IR (KBr) 341 1 , 2968. 1601 , 1489 cm " 1 : Cl MS m/z = 432 [C 2 4H 2 gN 7 +Hf. Example 15 - Preparation of Compound 19 To a solution of /.-BuLi (2.5M hexane solution. 10.9 mL. 27.4 mmol) in ethyl ether 28 mL at -78 °C was added 2-bromopyridine (4.33 g. 27.4 mmol) in ethyl ether ( 15 mL). After stirring for 30 min. a solution of trimeth\ lstannylchloride (6.0 g. 30 mmol) in THF (1 0 mL) was added. Stirring was continued at -78 °C for 2 h and the mixture was then warmed up to room temperature and filtered. The precipitate was washed ith ether and the combined the ether filtrates were concentrated to give the crude product: Η NMR (500 Hz. CDCh) δ 8.69-8.68 (d. 1 H). 7.47-7.07 (m. 3 FI). 0.30 (s. 9 H). Example 16 - Preparation of Compound 21 A mixture of 4-bromobenzonitrile (1.68 g. 9.2 mmol). crude 2- trimethylstannylpyridine (3.33 g. 13.8 mmol). and PdC l (PPh ) 2 (321 mg. 0.46 mmol) in DMF (25 mL) was heated at 150-155 °C in pressure tube for 24 h. The DMF was distilled off under reduced pressure and the residue was filtered through a short column of basic alumina and washed with ethyl acetate and then concentrated. Flash chromatography of the residue on silica gel gave the product (41 %) as a white solid: mp 99- 100 °C; Η NMR (500 Hz. CDC1 3 ) δ 8.74 (dd. Ji = 1 Hz. L = 1 .7 Hz. 1 FI). 8.12 (d. J = 8.6 FIz. 2 FI). 7.83-7.76 (m. 4 H), 7.32 (m. 1 H). Example 17 - Preparation of Compound 22 To LiAlH (8 mmol) in THF (25 mL) was added 21 (0.96 g. 5.3 mmol) in THF ( 15 mL) slowly while the flask was cooled with ice. The mixture was stirred at room temperature for 10-30 min then stirred at reflux for 4 h under nitrogen. The mixture was cooled in an ice bath and aqueous sodium hydroxide solution (0.5 mL, 10%)) was added. The mixture was stirred until the residue became white and the solid was filtered and washed with methylene chloride (4 x 5 mL). The methylene chloride solution was dried with anhydrous sodium sulfate, concentrated, and the crude product was chromatographed on silica gel to give the product as a yellow liquid. A small amount of ethanol was added and the pure amine 22 was obtained as a white solid (74%) after filtration: mp 1 14-1 17 °C; Η NMR (500 Hz. CDC1 3 ) δ 8.66 (d. J - 4.4 Hz. 1 FI). 7.94 (d. J = 8.1 Hz. 2 H). 7.70 (m. 2 H). 7.39 (d. J = 8.0 Hz). 7.19 (m. I H). 3.90 (s. 2 H). 1.98 (s. 2 H). Example 18 - Preparation of Compound 23 A mixture of 2.6-dichloropurine (1. 0.19 g. 1 mmol). amine 22 (0.39 g. 2.15 mmol) in ethanol (13 mL). and water (3.4 mL) was heated at 100-1 10 °C under nitrogen for 24 h and then it was cooled to room temperature. The mixture was concentrated and water (5 mL) was added. A solid was filtered and washed with water (2 x 5 mL) and dried under vacuum to give the product (93%) as yellow solid: mp 260 °C (dec): Η NMR (500 Hz. DMSO-J 6 ) δ 12.4 (bs. 1 H). 8.76 (m. J = 1 Hz. 1 H). 8.28 (s. 1 H). 8.16 (d. J = 8.1 Hz. 2 FI). 8.03 (d. J = 7.8 Hz. 1 H). 7.97 (m. 1 H). 7.58 (d = 8.6 Hz. 2 H), 7.45 (m, 1 FI). 4.82 (s. 2 H). Example 19 - Preparation of Compound 24 To the solution of compound 23 (0.33 g. 1 mmol) in DMSO (5.2 mL). added potassium carbonate (0.7 g. 5 mmol) and 2-iodopropane (0.5 g. 3 mmol). The mixture was stirred at ambient temperature under nitrogen for 24 h and poured into ice water (30 mL). After filtration, the solid was washed with water (4 x 5 mL). dried under vacuum to give the crude product as a yellow solid. Flash column chromatography of the crude product on silica gel and recrystallization provided the pure product (76%) as white crystals: mp 178-179 °C; Η NMR (500 FIz, CDC1 3 ) δ 8.68 (m. 1 H), 7.96 (d, J = 8 Hz, 2 H). 7.76-7.70 (m. 2 H), 7.73 (s, 1 FI). 7.47 (d, J = 8 Hz, 2 H), 7.22 (m. 1 H). 4.89 (s, 1 FI). 4.79 (m. 1 H). 1.54 (d. J = 6.8 Hz, 6 H); Cl MS m/= = 379 [C 2 oHi9ClN 6 +H] + . Anal. Calcd. for C 20 H I QC1N 6 : C. 63.41 ; FI. 5.05; N. 22.18. Found: C. 63.07; FI, 5.01 ; N, 22.01. Example 20 - Preparation of Compound 17 To compound 24 (0.7 g. 1.8 mmol) was added (R)-(-)-2 amino- 1 - butanol (3.5 g. 3.9 mmol) stirred in a sealed tube for 2 h at 190 °C. The reaction mixture was allowed to cool and then was partitioned between EtOAc and brine. The EtOAc was separated, washed with saturated brine (4 x), dried w ith Na 2 S0 4 . and concentrated. The product was air dried to gi\ e an oil. then dissolved in EtOAc. The EtOAc solution was cooled again, and the precipitate collected, washed with cold EtOAc (2 x). air dried, and heated in vacuo for 2 h to gi\ e 17 (0.54 g. 67%): mp 98- 100 °C; Η NMR (300 MHz. CDCh) δ 8.00-7.85 (m. 2 H). 7.75-7.55 (m. 2 H). 7.50- 7.35 (m. 3 FI). 7.30-7.15 (m. 1 FI). 6.40-6.20 (bs. 1 H). 5.00-4.82 (m. 1 H). 4.80-4.68 (bs. 3 H). 4.60 (heptuplet. 1 H). 3.98-3.70 (m. 2 FI). 3.70-3.54 (dd. 1 H). 2.10 (bs. 1 H). 1.75-1.53 (m. 2 H). 1.51 (d. 6 H). 1.00 (1. 3 H): IR (KBr) 3406. 2969. 1601. 1490. 1389. 1254. 779 cm " 1 ; API MS m/z = 432 [C 24 I l2«.N 7 O+H] " . Example 21 - Preparation of Compound 25 To compound 4 (0.14 g. 0.33 mmol) was added 3- (tributylstannyl)pyridine (0.15 g. 0.33 mmol). Pd(PPh ) 4 (0.06 g. 0.41 mmol). and toluene (10 mL). The solution was degassed with argon for 8 min in a sealed tube. and heated in an oil bath for 3 h at 130 °C. The cooled reaction mixture was diluted with saturated NaHCU 3 and extracted with CFLCL (3 x 50 mL). The combined organic extracts were washed with brine and dried over Na 2 S0 . The reaction mixture was purified by column chromatography on silica gel to give the desired coupling product. The product was dissolved in acetonitrile and washed with hexane (3 x 10 mL) to remove a portion of the tin contaminants. The reaction mixture was again purified by column chromatography on reversed phase silica gel to give compound 25 (0.04 g): Η NMR (300 MHz. CDC1 3 ) δ 8.83 (s. 1 FI). 8.58 (d. 1 H). 7.88-7.83 (m. 1 H), 7.56-7.46 (m. 5 FI). 7.38-7.33 (m. 1 FI). 5.99 (s, 1 FI), 5.1 1 (s, 1 H). 4.90-4.83 (m, 2 H), 4.63-4.56 (m. 1 FI). 3.92-3.81 (m. 2 H), 3.67-3.60 (m, 1 H), 1.69-1.49 (m, 8 FI). 1.05-1.00 (t, 3 FI); Cl MS m/z = 432 [C 24 H2 9 N 7 O+H] + . Example 22 - Preparation of Compound 27 A mixture of diethyl(3-pyridyl)borane (26, 540 mg, 3.67 mmol), 4- bromobenzonitrile (803 mg. 4.41 mmol) and Pd(PPh 3 ) (144 mg. 0.13 mmol) in toluene (9 mL). ethanol (1.3 mL) and 2M aqueous sodium carbonate solution (4.1 mL. 8.2 mmol) was heated at 90-100 °C under nitrogen for 27 h. The mixture was cooled to room temperature and water ( 10 mL) was added. The organic layer was separated and the aqueous layer was extracted with ethyl acetate (2 x 20 mL). The combined organic layers were washed with brine (2 15 mL) and dried over anhydrous sodium sulfate. Flash chromatography of the crude product on silica gave the product as a white solid (80%): mp 95-96 °C. Example 23 - An Alternative Preparation of 27 is Described Below A flask charged with 4-bromobenzonitrile (360 mg. 2.0 mmol). bis(pinacolato)diboron (560 mg. 2.2 mmol). potassium acetate (590 mg. 6.0 mmol) and PdCL(dppf) (49 mg. 0.06 mmol) was flushed with nitrogen and DMF (12 mL) was added. The mixture was heated at 80-85 °C for 4 h and then cooled to room temperature at which time PdCL(dppf) (49 mg. 0.06 mmol). 3-bromopyridine (385 δL. 3.40 mmol). and 2M aqueous sodium carbonate solution (5 mL. 10 mmol) was added. The mixture was stirred at 80-85 °C for 24 h and extracted with ethyl ether (3 x 30 mL) and then washed with brine (3 x 15 mL) and dried with anhydrous sodium sulfate. Flash chromatography of the crude product on silica gel gave the product as white crystals (56%): mp 96-97 °C; Η NMR (500 Hz. CDC1 3 ) δ 8.55 (dd. J, = 1 Hz, J = 1.4 FIz. 1 FI). 8.66 (m. 1 H). 7.90-7.87 (m. 1 H), 7.77 (d, J = 7.8 Hz. 2 H). 7.69 (d. J = 8.8 Hz. 2 FI), 7.42 (m, 1 H). Example 24 - Preparation of Compound 28 To LiAlH (8 mmol) in THF (25 mL) was added 27 (0.96 g. 5.3 mmol) in THF (25 mL) slowly while the flask was cooled with ice. The mixture was stirred at room temperature for 10-30 min then stirred at reflux for 4 h under nitrogen. The mixture was cooled in an ice bath and aqueous sodium hydroxide solution (0.5 mL, 10%) was added. The mixture was stirred until the residue became white and the solid was filtered and washed with methylene chloride (4 x 5 mL). The methylene chloride solution was dried with anhydrous sodium sulfate. concentrated, and the crude product was chromatographed on silica gel to give the product as a yellow liquid. A small amount of ethanol was added and the pure amine 28 was obtained as a white solid (46%) after filtration: mp 94-96 °C; Η NMR (500 Hz. CDCh) δ 8.74 (d. J = 2.4 Hz. 1 FI). 8.48 (dd. J, = 1.5 Hz. J 2 = 4.7 Hz. 1 H). 7.77 (m. 1 FI). 7.45 (d. J = 8.10 Hz. 2 FI). 7.33 (d. J = 8.0 Hz. 2 H). 7.25 (m. 1 FI). 3.83 (s. 2 H). 2.25 (s. 2 H). Example 25 - Preparation of Compound 29 A mixture of 2.6-dichloropurine (1. 0.19 g. 1 mmol). amine 28 (0.4 g. 2.15 mmol) in ethanol ( 13 mL). water (3 mL) was heated at 100-1 10 °C under nitrogen for 24 h and then it was cooled to room temperature. The mixture was concentrated and water (5 mL) was added. A solid was filtered and washed with water (2 x 5 mL) and dried under vacuum to give the product (92%) as a yellow solid: mp 21 °C (dec): Η NMR (500 Hz. DMSO-J 6 ) δ 13.2 (bs. 1 H). 8.99 (s. 1 FI). 8.66 (d. J = 3.5 Hz. 1 H). 8.28 (s. 1 FI). 8.16 (d. J = 7.3 Hz. 1 FI). 7.80 (d. J = 7.6 Hz. 2 H). 7.60-7.57 (m. 3 H). Example 26 - Preparation of Compound 30 To a solution of 29 (0.3 g. 1 mmol) in DMSO (5 mL). was added potassium carbonate (0.7 g. 5 mmol) and 2-iodopropane (0.5 g. 3 mmol). The mixture was stirred at ambient temperature under nitrogen for 24 h and poured into ice water (30 mL). After filtration, the solid was washed with water (4 x 5 mL). dried under vacuum to give the crude product as a yellow solid. Flash column chromatography of the crude product on silica gel and recrystallization provided the pure product (76%) as white crystals: mp 178-179 °C: Η NMR (500 Hz, CDC1 3 ) δ 8.82 (d, J = 1.3 FIz. 1 FI). 8.59-8.58 (m. 1 FI), 7.86-7.84 (m. 1 H), 7.72 (s. 1 H). 7.56- 7.48 (m. 4 FI), 7.37-7.34 (m, 1 H), 4.88 (s. 2 FI). 4.82 (m, 1 FI). 1.56 (d. J = 0.7 Hz. 3 H). 1.55 (d. J = 0.8 Hz. 3 FI); Cl MS m/z = 379 [C 2 oH| 9 ClN 6 +H] + . Anal. Calcd. for C 2 oHι 9 ClN 6 : C, 63.41 ; H. 5.05; N. 22.18. Found: C. 63.24; H. 4.97; N. 21.93. Example 27 - Preparation of Compound 32 To a mixture of 4 (0.05 g. 0.1 1 mmol) was added 4- (tributylstannyl)pyridine (0.06 g. 0.16 mmol), Pd(PPh ) 4 (0.02 g. 0.02 mmol). and toluene (2.5 mL). The reaction mixture was degassed and heated in a sealed tube at 125 °C for 3 h. The reaction mixture was cooled to room temperature then saturated NaHCO 3 (30 mL) was added followed by extraction with CH C1 2 (3 x 30). The organic layer was washed with brine (50 mL). dried with MgS0 4 . and concentrated. The reaction mixture was purified by column chromatography on silica gel to give 32: Η NMR (300 MHz. CDC1 3 ) δ 8.65 (s. 2 H). 7.60-7.57 (m. 2 H). 7.49-7.45 (m. 5 H). 6.20 (s. 1 H). 4.93 (d. 1 H). 4.84 (s. 2 H). 4.65-4.57 (m. 1 H). 3.92-3.80 (m. 2 H). 3.68-3.51 (m. 1 H). 1.68-1.58 (m. 2 H). 1.52 (d. 6 H). 1.05-0.99 (t. 3 H). Example 28 - Preparation of Compound 33 To compound 4 (0.18 g. 0.43 mmol) was added 4-vinylphenylboronic acid (0.19 g. 1.28 mmol). Pd(PPh 3 ) 4 (0.09 g. 0.08 mmol). Na 2 CO 3 (2M. 0.85 mL). was added toluene (5 mL). The mixture was degassed with argon for 10 min. The resulting solution was heated in a sealed tube at 135 °C for 4.5 h. The cooled solution was diluted with water and extracted with CHiCL (3 x 50 mL). The combined organic extracts were washed with brine and dried over Na2S0 4 . The solution was purified by flash column chromatography (2 x) on silica gel to give the desired product 33 as a yellow solid (0.09 g): mp 130-131 °C; Η NMR (300 MHz. CDC1 3 ) δ 7.57-7.42 (m. 9 H). 6.80-6.70 (dd, 1 H). 5.98 (s. 1 H). 5.79 (d, 1 H). 5.27 (d. 1 H). 4.88 (d. 1 FI). 4.84-4.72 (m. 2 FI), 4.63-4.56 (m, 1 FI). 3.92-3.81 (m. 2 H), 3.66-3.60 (m, 1 H). 1.68-1.52 (m. 8 H), 1.05-1.00 (t. 3 H); IR (CFLCL) 3293. 2968. 1601. 1489. 1390 cm "1 ; Cl MS m/z = 457 [C 27 FI 2 N 6 0+H] + . Example 29 - Preparation of Compound 34 To compound 33 (0.008 g, 0.016 mmol) was added OsO 4 (0.007 g, 0.026 mmol), pyridine (0.08 mL), and toluene (0.75 mL). The reaction mixture was stirred at room temperature in the dark for 1 h. concentrated in vacuo. and then slurried in methanol/water (9: 1 ). Sodium metabisulfite (0.07 g) was added and the reaction was stirred for 1 h. The mixture was washed with brine, extracted with CH 2 CI 2 (3 x 10 mL), dried over Na 2 S0 4 . and concentrated. The product was purified by column chromatography on silica gel to give compound 34 (0.003 g) as a tan solid: Η NMR (300 MFIz. CDCI 3 ) δ 7.51 (s. 1 H). 7.43-7.35 (m. 6 FI). 7.25-7.22 (m. 2 H). 6.51 (s. 1 FI). 4.98 (d. 1 H). 4.35-4.25 (m. 2 FI). 4.64-4.54 (m. 1 FI). 3.93-3.80 (m. 3 H). 3.74-3.59 (m. 3 FI). 1.68-1.58 (m. 2 H). 1.52 (d. 6 H). 1.06-0.99 (t. 3 H). Example 30 - Preparation of Compound 36 To compound 4 (0.12 g. 0.27 mmol) was added 3-aminophenylboronic acid hydrochloride (0.12 g. 0.69 mmol). and Pd(PPh ,) 4 (0.09 g. 0.75 mmol) in a sealed tube filled with argon. To this mixture was added toluene (5 mL) and Na 2 CO , (2M. 0.55 mL). The resulting solution was degassed with argon for 5 min and placed in a 130 °C oil bath for 6 h. The cooled solution was diluted with water and extracted with CHiCL (3 x 50 mL). The combined organic layers were washed with brine, dried over Na 2 S0 4 . and concentrated. The solution was purified by column chromatography on silica gel to yield 36 (0.04 g. 36%): Η NMR (300 MHz. CDCl;,) δ 7.52-7.46 (m. 3 FI). 7.39 (d. 2 FI). 7.23-7.18 (m. 1 H). 6.96 (d. 1 H). 6.88 (t. 1 H), 6.68-6.66 (m. 1 H). 6.12 (s. 1 H). 4.90 (d. 1 H). 4.79 (s. 2 FI), 4.62-4.57 (m. 1 H). 3.92-3.76 (m. 4 H). 3.66-3.60 (m. 1 FI). 1.65-1 .48 (m, 8 FI). 1.04-0.99 (t. 3 H): Cl MS m/z = 446 [C 25 H 31 N 7 O+H] + . Example 31 - Preparation of Compound 38 To a suspension of Pd(PPli . 4 (0.02 g. 0.01 mmol) in anhydrous DME (8 mL) was added 4 (0.12 g. 0.27 mmol) and the mixture stirred at room temperature for 10 min. To this solution was added 3-(trifluoromethyl)phenylboronic acid (37; 0.12 g. 0.65 mmol) in a minimum of EtOH, followed by Na 2 CO 3 (2M. 0.27 mL). and the resulting mixture was heated at reflux for 20 h. The cooled reaction mixture was diluted with water and extracted with CH 2 CL (3 x 50 mL). The combined organic layers were washed with brine, dried over Na 2 S0 , and concentrated. The reaction mixture was purified by column chromatography on normal phase silica gel followed by reversed phase column chromatography to obtain 38 (0.04 g. 33%>) as an off white solid: mp 60-67 °C; 1H NMR (300 MHz. CDCI 3 ) δ 7.81 (s. 1 H). 7.74 (d. 1 FI), 7.58- 7.45 (m. 7 FI). 5.98 (s. 1 H). 4.90-4.83 (m. 3 FI). 4.63-4.59 (m. 1 H). 3.90-3.81 (m. 2 FI). 3.66-3.60 (m. 1 FI). 1.68-1.51 (m. 8 H). 1.05-1.00 (t. 3 H); IR (KBr) 3406. 2969, 1602. 1489. 1335 cm - 1 ; Cl MS m/z = 499 Example 32 - Preparation of Compound 40 A mixture of 4 (0.13 g. 0.31 mmol). 2-naphthaleneboronic acid (39; 0.1 1 g. 0.62 mmol) and Pd(PPh .) 4 (0.09 g. 0.08 mmol ) was placed in a sealed tube that was filled with argon. To the mixture was added toluene (5 mL) and Na 2 CO 3 (2M. 0.62 mL). The tube was quickly sealed and heated at 125 °C in an oil bath for 6 h. The cooled solution was diluted with water and extracted with CFLCL (3 x 50 mL). The organic layers were washed with brine, dried over Na 2 S0 4 . and concentrated. The reaction mixture was purified by column chromatography on normal phase silica gel. followed by reversed phase chromatography to give 40 (0.04 g. 28%): mp 70-75 °C: Η NMR (300 MHz. CDCh) δ 8.02 (s. 1 H). 7.92-7.84 (m. 3 H). 7.74-7.67 (m. 3 H). 7.51-7.44 (m. 5 H), 5.96 (s. 1 H). 4.89-4.84 (m. 3 FI). 4.66- 4.57 (m. 1 H). 3.93-3.82 (111. 2 H). 3.67-3.61 (in. 1 H). 1.76-1.50 (m. 8 FI). 1.06-1.01 (t. 3 H): IR (KBr) 3422. 2927. 1601. 1491. 1388 cm ' 1 . Example 33 - Preparation of Compound 43 To compound 4 (0.14 g. 0.33 mmol) was added 4- methoxyphenvlboronic acid (42. 0.1 1 g. 0.71 mmol). Pd(PPh ) 4 (0.10 g. 0.087 mmol). Na 2 CO 3 (2M. 0.66 mL). and toluene (7 mL). The solution was degassed for 8 min with argon and heated in an oil bath at 125 °C for 6 h. The cooled solution was diluted with water and extracted with CH 2 CI 2 (3 x 50 mL). The combined organic layers were washed with brine, dried over Na 2 SO 4 , filtered, and concentrated. The reaction mixture was purified by normal phase column chromatography followed by reversed phase chromatography to give 43 (0.05 g. 28%) as a white solid: mp 128- 130 °C; Η NMR (300 MHz. CDC1 3 ) δ 7.52-7.50 (m. 5 H). 7.41 (d. 2 H), 6.97 (d, 2 FI). 5.93 (s, 1 H). 4.89-4.79 (m. 3 FI), 4.63-4.56 (m, 1 H). 3.92-3.81 (m, 5 H), 3.67- 3.60 (m, 1 H). 1.68-1.49 (m, 8 H), 1.05-1.00 (t, 3 FI); IR (KBr) 3417. 2931. 1610. 1499. 1389 cm " ' ; CI MS ..?/-. = 461 [C2 6 H 3 2N 6 θ2+H " r. Example 34 - Preparation of Compound 45 To a solution of ..v-BuLi (5 mL. 6.24 mmol) and TMEDΛ ( 1 mL) in anhydrous THF (35 mL) at -75 °C under argon was added dropwise a solution of Λ ' N- dieth} lbenzamide (0.98 g. 5.57 mmol) in THF (5 mL). The mixture was stirred for 50 min and then treated with trimethylborate (2 mL. 17 mmol). The solution was allowed to warm to room temperature overnight. The colorless solution was cooled to 0 °C and acidified to pFI = 6 with 2Ν FIG. The TFIF was removed in vacuo and the residue was diluted with water. This was extracted with CH2CL (3 x 50 mL) and the combined organic layers were washed with brine, dried over Na 2 S0 4 . concentrated in vacuo. followed by removal of trace solvent on the vacuum pump to give 45 as an off- white foamy solid: Η NMR (300 MFIz. CD ,OD) δ 7.67-7.39 (m. 4 FI). 3.88-3.69 (q. 4 PI). 1.41 -1.30 (t. 6 H). Example 35 - Preparation of Compound 46 To compound 4 (0.14 g. 0.31 mmol) was added 2- (diethylcarbamoyl)phenylboronic acid (45. 0.29 g. 1.31 mmol). Pd(PPh 3 ) 4 (0.1 g. 0.09 mmol). Na 2 C0 3 (2M. 0.63 mL). toluene (5 mL), and the mixture degassed with argon for 10 min. The mixture was heated in an oil bath for 5 h at 135 °C. The cooled solution was diluted with water and extracted with CH 2 CL (3 x 50 mL). The organic layers were combined, washed with brine, dried over Na 2 Cθ . and concentrated. The reaction mixture was purified by normal phase column chromatography on silica gel. followed by reversed phase chromatography to give 46 (0.03 g. 18%) as a yellow solid: Η NMR (300 MFIz. CDCI 3 ) δ 7.49-7.36 (m, 9 H). 6.18 (s. 1 H), 4.93 (d. 1 H). 4.78 (s. 2 H). 4.64-4.55 (m. 1 H), 3.92-3.60 (m. 4 H). 3.06-2.92 (m. 2 H). 2.69-2.64 (m. 1 FI). 1.68-1.51 (m, 8 FI), 1.04-0.99 (t. 3 H), 0.91 -0.86 (t. 3 H), 0.77-0.72 (t. 3 H); Cl MS m/z = 530 [C 30 Fl 3 9N 7 O 2 +H] + . Example 36 - Preparation of Compound 48 To a suspension of Pd(PPh 3 ) (0.08 g. 0.69 mmol) in DME was added 4 (0.129 g, 0.30 mmol) and the mixture stirred for 10 min at room temperature. To this was added 3-nitrophenylboronic acid (47. 0.157 g. 0.94 mmol) and Na 2 Cθ 3 (2 M. 0.59 mL). The solution was heated at reflux under argon overnight. The cooled solution was diluted with water and extracted with CH2CI2 (3 x 50 mL). The organic layers were combined, washed with brine, dried over Na2S0 4 . and concentrated in vacuo. The solution was purified by flash column chromatograph} on silica gel to give 48 (0.04 g. 29%) as a bright yellow solid: mp 73-77 °C: Η NMR (300 MHz. CDCI 3 ) δ 8.43 (s. 1 H). 8.20 (d. 1 H). 7.89 (d. 1 H). 7.63-7.43 (m. 6 H). 6.01 (s. 1 H). 4.95-4.76 (m. 3 FI). 4.68-4.58 (m. 1 H), 3.98-3.80 (m. 2 H). 3.68-3.60 (m. 1 H). 1.71 - 1.40 (m. 8 FI). 1.02-0.98 (t. 3 FI): IR (KBr) 3405. 2930. 1713. 1602. 1490. 1351 cm "1 ; Cl MS m/z = 476 [C 25 H 2l ,N 7 O 3 +H] " . Example 37 - Preparation of Compound 50 To a suspension of Pd(PPli 3 ) 4 (0.09 g. 0.08 mmol) in DME (5 mL) was added 4 (0.14 g. 0.32 mmol) and the mixture stirred at room temperature for 15 min. To this was added benzo[b]furan-2-boronic acid (49. 0.153 g, 0.94 mmol) and Na 2 Cθ3 (2 M. 0.63 mL). The solution was heated at reflux under argon overnight. The reaction mixture was cooled, diluted with water, extracted with CH2CI 2 (3 x 50 mL). The organic layers were combined, washed with brine, dried over Na 2 SO 4 . and concentrated in vacuo. The solution was purified by flash column chromatography on silica gel followed by flash column chromatography on reversed phase silica to give 50 (0.09 g. 60%) as a white solid: 1H NMR (300 MFIz. CDCI3) δ 7.82 (d. 2 FI). 7.58- 7.42 (in. 5 H). 7.30-7.19 (m. 2 FI). 7.01 (s. 1 H), 6.1 1 (s. 1 H). 4.91 (d. 1 H), 4.81 (s, 2 H), 4.62-4.58 (m. 1 H), 3.92-3.80 (m, 2 H), 3.66-3.60 (m. 1 H). 1.66-1.48 (m, 8 H), 1.04-0.99 (t, 3 FI); Cl MS m/z = 471 [C 27 Fl oN 6 θ 2 +H] + . Example 38 - Preparation of Compound 52 To compound 4 (0.46 g, 1.20 mmol) was added 1 -amino-l - cyclopentanemethanol (51, 1.0 g. 8.61 mmol) and EtOH (2 mL) and the mixture was heated in an oil bath at 150 °C for 60 h. The brown solution was cooled and heated again at 150 °C for 48 h. The reaction mixture was cooled and concentrated in vacuo. The reaction mixture was purified by flash column chromatograph}' on silica gel to give 52 (0.39 g. 71 %) as a tan solid: Η NMR (300 MFIz. CDCI 3 ) δ 7.48-7.40 (m. 3 H). 7.29-7.20 (m. 2 H). 6.88 (s. 1 H). 6.25 (s. 1 H). 5.10 (s. 1 H). 4.72 (s. 2 H). 4.63- 4.51 (ni. 1 H). 3.78 (s. 2 H). 2.10-1.65 (m. 8 H). 1.54 (d. 6 H): Cl MS m/z = 459 Example 39 - Preparation of Compound 53 To a suspension of Pd(PPh ) (0.07 g. 0.06 mmol) in DME (5 mL) was added 52 (0.102 g. 0.22 mmol) and stirred at room temperature for 15 min. To this was added phenylboronic acid (0.098 g. 0.80 mmol) and Na 2 C0 3 (2 M. 0.44 mL). The solution was heated at reflux under argon for 18 h. The reaction mixture was diluted with water, extracted with CH 2 CI 2 (3 x 50 mL). washed with brine, and dried over Na S0 . The solution was purified by flash column chromatography on silica gel followed by flash column chromatography on reversed phase silica gel to give 53 (0.02 g. 20%): Η NMR (300 MHz. CDC1 3 ) δ 7.59-7.31 (111. 10 H). 6.95 (s. 1 H). 5.95 (s. 1 FI). 5.10 (s. 1 H). 4.79 (s. 2 FI). 4.61-4.52 (m. 1 H). 3.76 (s. 2 H). 2.01-1.61 (m. 8 FI). 1.54 (d, 6 H); Cl MS m/z = 457 [C2 7 H 3 2N 6 0+Hf . Example 40 - Preparation of Compound 54 To compound 3 (0.26 g. 0.67 mmol) was added tran.s-4- aminocyclohexanol hydrochloride (0.62 g, 4.1 1 mmol). Et 3 N (0.58 mL. 4.16 mmol). and ethanol (5 mL). The mixture was heated for 5 h at 135 °C in an oil bath. The temperature increased to 150 °C and heating was continued for a further 48 h. The solution was cooled and evaporated to give a yellow oil: Cl MS m/z = 459 Example 41 - Preparation of Compound 55 To compound 3 (0.50 g. 1.31 mmol) was added cis- 2- diaminocvclohexane (1.57 mL. 13.1 mmol) and EtOFI (4 mL). The mixture was heated in an oil bath at 150 °C for 6 h. The reaction mixture was concentrated in vacuo. The reaction mixture was purified by column chromatography on silica gel to give 55 (0.49 g. 82%) as a yellow solid: Η NMR (300 MFIz. CDC1 3 ) δ 7.43-7.40 (111. 3 H). 7.23 (d. 2 FI). 6.21 (s. 1 FI). 5.04 (d. 1 FI). 4.72 (s. 2 FI), 4.67-4.58 (m, 1 H). 4.08-4.05 (nι. 1 FI). 3.17-3.15 (m. 1 H). 2.08 (s. 2 H). 1.65-1.38 (m. 14 FI): Cl MS m/z = 458 [C 2 |H 28 BrN-.+Hf. Example 42 - Preparation of Compound 56 To compound 55 (0.10 g. 0.22 mmol) was added 2- (tribut} lstannyl)pyridine (0.10 g. 0.27 mmol). Pd(PPh 3 ) 4 (0.05 g. 0.04 mmol). and toluene (5 mL). The solution was degassed with argon for 8 min and heated at 135 °C for 3 h. The cooled solution was diluted with water, extracted ith CFLCF (3 x 50 mL). and the combined organic extracts were washed with brine, dried over Na 2 S0 4 . filtered, and concentrated. The solution was followed b} flash column chromatography (2 x) to give the desired product 56 (0.03 g. 36%) yellow crystalline solid: Η NMR (300 MHz. CDCF,) δ 8.68 (d. 1 FI). 7.96 (d. 2 H). 7.78-7.69 (m, 2 H). 7.49 (s. 1 H). 7.44 (d. 2 H). 7.23-7.18 (m. 1 FI). 6.10 (s. 1 FI). 5.10-5.00 (m, 1 FI). 4.83 (s. 2 H). 4.69-4.60 (m. 1 H). 4.20-4.10 (m. 1 H), 3.27-3.13 (m. 1 FI). 2.48 (s. 2 H). 1.78-1 .42 (m. 14 H); Cl MS m/z = 457 [C 2 6H 32 N 8 -i-Hr. Example 43 - Preparation of Compound 57 To compound 1 (0.50 g. 1.31 mmol) was added .ram- 1 ,2- diaminocyclohexane (2.52 mL. 21 mmol). and EtOFI (6 mL). The reaction mixture was placed in an oil bath and heated to 190 °C for 25 h. The reaction mixture was removed from the heat and cooled to room temperature, concentrated for purification. The reaction mixture was purified by column chromatograph} on silica gel to yield 57 (520 mg, 87%) as an off white foam: Η NMR (300 MHz, DMSO) δ 7.95 (bs. 1 H). 7.85 (s. 1 FI). 7.50 (d. 2 H). 7.34 (d, 2 FI). 6.17 (d, 1 FI). 4.70-4.40 (ni. 1 FI). 2.00-1.71 (m. 4 H), 1.70-1.52 (m. 2 FI). 1.41 (d. 6 H). 1.30-0.92 (m. 4 PI): API MS m/z = 460 Example 44 - Preparation of Compound 58 Compound 57 (0.15 g, 0.32 mmol) was added to a suspension of Pd(PPli 3 ) 4 (0.1 1 g. 0.1 mmol) in DME (7 mL) and stirred at room temperature for 15 min. Phenylboronic acid (0.14 g. 1.14 mmol) was added followed by the Na CU 3 (2M. 0.62 mmol). The reaction mixture was refluxed under argon for 18 h and allowed to stir at room temperature for 51 h. It was then diluted with water, extracted with CH 2 CI 2 . washed with brine, and then extracted with CPLCF. The organic layer was evaporated, dried over anhydrous Na 2 S0 4 . purified by column chromatograph}'. and placed in vacuo for 18 h to give 58 (0.10 g. 72%) as a white solid: Η NMR (300 MFIz. CDCI 3 ) δ 7.62-7.35 (m. 10 H). 5.92 (br. 1 FI). 4.83 (br. 2 H). 4.74-4.56 (m. 2 H). 3.77-3.55 (m. 1 IT). 2.55-2.43 (m, 1 H). 2.16-1.91 (m. 2 H ). 1.73 (br. 2 H). 1.52 (d. 6 H). 1.37-1.09 (m. 6 PI): API MS m/z = 456 Example 45 - Preparation of Compound 59 To compound 57 (460 mg. 1 .0 mmol) in solution with CfLCF (2 mL) was added acetic anhydride (0.44 mL. 4.6 mmol). catalytic DMAP. and pyridine (0.5 mL). The mixture was stirred at room temperature for 2.5 h. The mixture was diluted with CH 2 CI 2 . washed with 2N HC1. and the combined organics were then washed with NaHCθ . The organics were then washed with brine, dried over Na 2 SO , filtered, and concentrated to give 59 (472 mg. 94%>) as an off white solid: Η NMR (300 MHz. DMSO-J 6 ) δ 7.76 (s. 1 H), 7.42 (d, 2 H), 7.29 (d. 2 H). 4.68-4.40 (m. 1 H). 4.10 (s. 3 FI), 3.61-3.40 (ni. 2 H). 2.15-1.80 (m. 2 FI), 1.74-1.55 (m. 4 H). 1.45 (d. 6 H). 1.35-1.05 (m. 4 H); API MS m/z = 500 [CYiHjoBrN.O+Hf. Example 46 - Preparation of Compound 60 To a suspension of Pd(PPli 3 ) 4 (0.1 1 g, 0.1 mmol) in DME (7 mL) was added compound 59 (0.15 g. 0.3 mmol) and stirred at room temperature for 15 min under argon. Phenylboronic acid (0.13 g. 1 .06 mmol) was added, followed by Na 2 CO 3 (2M, 0.62 mL). The reaction mixture was refluxed under argon for 18 h. The reaction mixture was then diluted with H 2 O, extracted with CH 2 CL, washed with brine, and extracted with CF CL. The organic layer was dried over anhydrous Na 2 S0 4 , purified by column chromatography. concentrated in vacuo for 18 h to yield 60 (61 mg. 42%): Η NMR (300 MHz. DMSO-J 6 ) δ 7.96 (s. 1 FI). 7.72 (s. 1 H). 7.51 (t. 3 H). 7.40-7.28 (m. 3 FI). 7.28-7.13 (m. 2 FI), 5.84 (br. 1 FI). 4.46 (br. 3 H). 3.47 (br. 2 FI). 1.83 (br. 1 FI). 1.62 (s. 4 H). 1.43 (d. 6 H). 0.12 (s. 3 FI); API MS m/z = 498 Example 47 - Preparation of Compound 61 To compound 3 (0.58 g. 1.53 mmol) was added tram- 1.4- diaminocyclohexane (1.78 g. 15.6 mmol). and EtOFI (4 mL). The mixture was heated in an oil bath at 150 °C for ca. 60 h. The reaction mixture was purified by column chromatograph} on silica gel to yield 61 (0.48 g. 68%>) as an off white solid: mp 122- 125 °C: Η NMR (300 MHz. CDC1 3 ) δ 7.43 (s. 1 H). 7.40 (d. 2 H). 7.20 (d. 2 H). 6.27 (s. 1 H). 4.75-4.68 (ni. 2 IT). 4.67-4.58 (m. 2 FI). 3.81 -3.68 (m. 1 H). 3.45 (s. 2 FI). 2.88-2.75 (m. 1 FI). 2.18-2.05 (m. 2 H). 2.05-1.89 (m. 2 H). 4.52 (d. 6 FI). 1.45-1.13 (m. 4 FI): Cl MS m/z = 459 [C iH 28 BrN 7 +H " T. Example 48 - Preparation of Compound 62 Amine 61 (53 mg, 0.12 mmol) was dissolved in CH 2 CL (2 mL) and pyridine (5 mL). Acetic anhydride (0.05 g, 0.53 mmol) and DMAP (few crystals) were added. The reaction mixture was allowed to stir at room temperature for 2.25 h. The reaction mixture was diluted with CH2CF. washed with 2N HC1. NaHC0 . dried over MgS0 . filtered, and evaporated to yield 62 (0.05 g. 78%) as a white solid: Η NMR (300 MHz. CDC1 3 ) δ 7.50-7.20 (m. 5 FI). 6.02 (br. 1 H). 5.29-5.20 (m. 1 FI). 4.72 (d. 2 H). 4.66-4.54 (m. 2 H), 3.72 (br, 2 H). 2.18-2.06 (m, 2 H). 2.06-1.91 (m. 2 H). 1.97 (s. 3 H). 1.54 (d, 6 H), 1.36-1.15 (m, 4 FI): API MS m/z = 500 Example 49 - Preparation of Compound 64 Compound 61 (0.05 g, 0.1 1 mmol) was dissolved in CFLCF (3 mL) and Et N (2 mL) and placed in an ice bath for 10 min. Compound 63 (0.06 g, 0.22 mmol) was dissolved in CH 2 CI 2 (2 mL). added dropwise. and rinsed with CFLCF (1.5 mL). The ice bath was removed after 20 min and the reaction was allowed to stir for 7 d. The reaction mixture was diluted with CH2CI2. washed with 2N FIC1 until the aqueous layer was acidic, washed with NaHCO .. dried over MgS0 . and evaporated. The desired product was isolated b}' column chromatography and dried in vacuo to yield 64 (0.04 g. 50%) as a green solid: Η NMR (300 MFIz. CDC1 3 ) δ 8.53 (d. 1 H). 8.32-8.20 (m. 2 FI). 7.59-7.35 (m. 4 H). 7.23-7.1 1 (m. 4 H). 6.02 (br. 1 H). 4.69-4.45 (m. 5 FI). 3.57 (br. 1 H). 3.12 (br. 1 FI). 2.87 (s. 1 FI). 1.97 (br. 2 H). 1.75 (br. 2 H). 5 1.48 (d. 6 H). 1.27-0.97 (m. 4 I I): API MS m/z = 693 Example 50 - Preparation of Compound 65 Compound 61 (0.05 g. 0.1 1 mmol) was dissolved in CH CI 2 (3 mL) 0 and Et 3 N (2 mL) and placed in an MeOFI/ice bath. Methanesulfonyl chloride (0.012 mg. 0.1 1 mmol) in CH 2 CI 2 (2.3 niF) was slowly added. The reaction mixture and ice bath was allowed to come to room temperature. After 1.5 h. the reaction mixture was diluted with CH 2 CI 2 . washed with 2N FIC1 until the aqueous layer was acidic. The organic layer was washed with NaFIC0 3 . dried over MgS0 4 . filtered, and evaporated. 5 The product was purified by column chromatography. and dried in vacuo for 14 h to yield 65 (13 mg. 24%) as an off-white solid: Η NMR (300 MFIz. CDCF,) δ 7.50-7.17 (m. 5 FI), 5.90 (br. 1 H). 4.75-4.57 (m. 3 H). 4.1 1 (d. 1 H). 3.69 (br. 1 H), 3.30 (br. 1 H). 2.99 (s. 3 H), 2.18-2.03 (m. 4 H). 1.69 (d. 6 H). 1.42-1.15 (m, 5 H); API MS m/z = 538 fC 22 H 3 oBrN 7 θ 2 S+H] + . 0 Example 51 - Preparation of Compound 66 Compound 61 (0.05 g, 0.1 1 mmol) was dissolved in toluene (4 mL). 2-Acetylphenylisocyanate (0.024 g. 0.15 mmol) diluted with toluene (1 mL) and -1 added to compound 61. Toluene (6 mL) was added to the reaction mixture. The reaction mixture was placed under reflux for 19 h. The product was purified by column chromatography, concentrated, and dried in vacuo for 23 h to yield 66 (42 mg. 62%) as an off-white solid: Η NMR (300 MFIz, CDC1 3 ) δ 7.87-7.20 (m. 9 H), 6.41 (s. 1 IT), 5.86 (br. 1 H). 4.75-4.54 (m. 4 H). 3.69 (br. 1 FI). 2.60 (s. 3 FI). 2.12 (br, 0 4 FI), 1.51 (d. 6 H). 1.42-1.15 (m. 5 FI); API MS m/z = 619 [C3oH . BrN 8 θ 2 +FI] + . Example 52 - Preparation of Compound 67 Compound 61 (0.04 g. 0.10 mmol ) was dissolved in CH 2 CF (2 mL) and pyridine (0.5 mL). Cyclopropanecarbonyl chloride (0.05 g. 0.44 mmol) was added along with DMAP (small amount). The reaction mixture was allowed to stir at room temperature for 2.25 h. The reaction mixture was diluted with CH CL. washed with 2N HC1. saturated NaHC0 , dried over MgS0 . filtered, and evaporated. The product was isolated by column chromatograph}' to yield 67 (0.03 g. 63%) as a white solid: Η NMR (300 MHz. CDCI 3 ) δ 7.50-7.20 (m. 5 FI). 5.96 (br. 1 H). 5.41 (d. 1 H). 4.72 (d. 2 H). 4.66-4.54 (m. 2 H). 3.72 (br. 2 FI). 2.18-1.97 (m. 4 FI). 1.51 (d. 6 H). 1.36-1.15 (m. 5 FI). 1 .06-0.88 (m. 2 FI). 0.79-0.67 (m. 2 H); API MS m/z = 526 Example 53 - Preparation of Compound 69 To a solution of 4-biphenylcarboxaldehyde (1.0 g. 5.49 mmol) in MeOH (20 mL) was added NaBH .CN (0.69 g. 1 1.0 mmol). and NH 4 OH (15 mL) and the mixture was stirred at room temperature overnight. To this added HC1 and extracted with CHCI 3 . The resulting aqueous layer was brought to pH > 7 with sodium bicarbonate and then extracted with CHCI 3 . The solution was dried with MgSO . filtered, and evaporated to give 69 (200 mg) as a white solid: El MS m/z = 183 [C, 3 Hi 3 N] + . Example 54 - Preparation of Compound 69 To compound 70 (2.75 g. 13.9 mmol) was added anhydrous THF (60 mL). heated to reflux, and kept under nitrogen. 1 M Borane-THF (69.7 mL) was added dropwise to 70 through an addition funnel resulting in a homogeneous solution. The solution was refluxed for 18 h. The reaction mixture was cooled in an ice water bath and quenched with H 2 O. 2N HC1 (20 mL). followed by 3N NaOH (60 mL). The reaction mixture was extracted with EtOAc (3 x). The organic extracts were washed with brine, and dried over sodium sulfate. The crude product was concentrated, dissolved in MeOFl, and FIC1 gas was bubbled through the solution. The solution was filtered in vacuo to give 69 as a white solid: Η NMR (300 MFIz. CDjOD) δ 7.71 (d. 2 FI). 7.63 (d. 2 H). 7.52 (d. 2 H). 7.47-7.30 (m. 3 H). 4.13 (s. 2 H ). Example 55 - Preparation of Compound 71 To compound 1 (6.8 g. 36.0 mmol) and 69 (8.0 g. 36.5 mmol) was added H 2 0 (60 L) and Hiinigs base (9.0 g. 70.0 mmol). T he mixture was stirred and heated to reflux for 5 h during which time FLO (50 mL) was added as the reaction continued to thicken. The crude product was collected by filtration, washed with H 2 0 (500 mL) and EtOH (2 x 30 mL). air dried, and dried in vacuo to give 71 (1 1.1 g. 92%): mp 267-269 °C. Example 56 - Preparation of Compound 72 Compound 71 (4.7 g. 14.0 mmol). K 2 CO 3 (15.0 g. 109 mmol). DMSO (80 mL). and 2-iodopropane (9.4 g. 55.0 mmol) were combined and stirred overnight. H 2 O and EtOAc were added. The EtOAc layer was separated and washed with brine (3 x). The EtOAc solution was dried with MgS0 . concentrated, and crystallized from EtOAc to give 72 (3.5 g, 66%): mp 139-140 °C. Example 57 - Preparation of Compound 73 Compound 72 (2.00 g. 5.30 mmol) and (R)-(-)-2-amino-l -butanol (10.8 g. 121 mmol) were combined in a sealed tube, and heated in an oil bath at 190 °C for 2 h. The solution was cooled to 60 °C. diluted in EtOAc. washed with brine (4 x), dried with Na 2 S0 . and concentrated. Purification by column chromatography on SiO 2 gave the desired product 73 (1.72 g. 75%) as a foam: Η NMR (300 MHz. CDCI 3 ) δ 7.65-7.10 (m. 9 H). 6.40-6.10 (bs. 1 H). 5.05-4.85 (m. 1 H). 4.85-4.67 (m. 1 FI). 4.60 (heptuplet. 1 FI). 4.00-3.70 (dd. 2 FI). 3.76-3.50 (m. 1 H). 1.95 (bs, 1 H), 1.80-1.55 (m. 2 H). 1.51 (d, 6 FI). 1.03 (t. 3 FI): IR (CFLCL) 3301. 2969. 1601. 1488. 1389. 1255. 762. 698 cm "1 ; API MS m/z = 431 Example 58 - Preparation of Compound 74 Compound 72 (0.23 g. 0.60 mmol). c..s-1.2-diaminocyclohexane (0.72 mL. 6.0 mmol). and ethanol (2 mL) were combined in a sealed tube and heated in an oil bath at 155 °C for 5 d. The ethanol was removed in vacuo and the crude reaction mixture was filtered through a silica plug. The reaction mixture was chromatographed on silica gel. the resulting orange solid was dissolved in CHiCL and a portion of activated charcoal was added. The solution was filtered through a pad of celite and concentrated to give 74 as a yellow solid (0.04 g. 27%): Η NMR (300 MHz. CDC1 3 ) 7.59-7.31 (m. 10 IT). 6.00 (s. 1 FI). 5.09 (d. 1 H). 4.83 (s. 2 H). 4.68- 4.62 (m. 1 FI). 4.1 1 (s. 1 H). 3.70-3.65 (m. 2 FI). 3.18-3.16 (m. 1 H). 2.02 (s. 2 FI). 1.67-1.42 (m. 12 FI); Cl MS m/z = 456 [C27H33N7+H] ' . Example 59 - Preparation of Compound 75 Compound 72 (0.17 g, 0.45 mmol), .ram-1.4-diaminocyclohexane (0.53 g, 4.69 mmol), and EtOFI (5 mL) were combined in a sealed tube and heated at 155 °C for 5 d. The EtOH was removed in vacuo and the crude mixture was subjected to flash chromatography on silica gel. Recrystallization from CHCL/MeOH gave 75 (5.8 mg) as an off-white crystalline solid: mp 1 10-1 12 °C; 1H NMR (300 MHz. CDCI 3 ) δ 7.58-7.31 (m. 10 FI). 5.95 (s. 1 H). 4.88-4.78 (m. 2 H). 4.69-4.60 (m, 2 H). 3.88-3.78 (m. 1 FI). 3.07-2.98 (m. 1 FI). 2.26-2.10 (m. 4 H). 1.62-1.52 (m. 8 FI). 1.29-1.15 (m. 4 FI); Cl MS m/z = 456 [C 27 H33N 7 +H] * . Example 60 - Preparation of Compound 76 Compound 75 (0.05 g. 0.1 1 mmol) was dissolved in CH 2 CL and the solution cooled to 0 °C under an argon atmosphere. A catalytic amount of DMAP, triethylamine (50 L, 0.36 mmol), followed by the acetyl chloride (25 L, 0.36 mmol) were added to the reaction mixture. The solution was warmed to room temperature and washed with NaFϊCO^ (5%). water, and brine. The solution was dried over Na 2 SO and concentrated. Purification by flash chromatography on silica gel gave 76 (0.028 g. 53%) as a pale yellow solid: mp 224-225 °C; Η NMR (300 MFIz. CDCI3) δ 7.59-7.31 (ni, 10 H). 5.93 (s. 1 FI). 5.26 (d. 1 H). 4.81 (s. 2 H). 4.65-4.58 (ni. 1 PI). 3.78-3.75 (111. 2 H). 2.18-1.99 (m. 4 H). 1 .95 (s. 3 FI). 1.77 (s. 1 FI). 1.53 (d. 6 H). 1.32-1 .22 (ni. 4 H): Cl MS m/z = 498 [C^PF-^O+Hf. Example 61 - Preparation of Compound 77 Compound 72 (0.15 g. 0.40 mmol). .ram-4-aminocyclohexanol hydrochloride (0.31 g. 1.99 mmol). Et N (0.1 1 mL. 0.8 mmol). and EtOFI (5 mL) were combined and heated in a sealed tube at 155 °C for 4 d. Additional irans-4- aminocyclohexanol hydrochloride (0.34 g. 2.2 mmol) and triethylamine (0.60 mL. 4.3 mmol) were added and the heat was resumed at 155 °C overnight. The crude product was purified by flash column chromatography to give 77 (0.036 g. 20%>) as an off- white solid: mp 196-200 °C: Η NMR (300 MHz. CDCF) δ 7.58-7.30 (m. 10 H). 5.97 (s. 1 H). 4.83-4.81 (m. 2 FI). 4.66-4.60 (m. 2 H), 3.82-3.77 (m. 1 FI). 3.69-3.62 (m, 1 FI), 2.17-2.13 (m. 2 H), 2.01 -1.97 (m. 2 H). 1.68 (s, 1 H). 1.53 (d. 6 H). 1.49-1.20 (m, 4 FI); Cl MS m/z = 457 [C 27 FL .N 6 0+H] + . Example 62 - Preparation of Compound 78 To compound 61 (0.12 g, 0.26 mmol). was added compound 16 (0.12 g. 0.33 mmol). and Pd(PPh 3 ) (0.06 g, 0.056 mmol) and toluene (5 mL). The resulting mixture was degassed for 10 min with argon. The mixture was heated at 140 °C for 3 h. The cooled solution was diluted with saturated NaFIC03 and extracted with CH 2 CL (3 x 50 mL). The combined organic extracts were washed with brine, dried over Na 2 SO . filtered, and concentrated to give a pale yellow oil which crystallized upon standing at room temperature. The crude product was purified by column chromatography and concentrated to give a white solid. The solid was precipitated with acetonitrile, filtered, washed with ether and hexane to give 78 (0.02 g. 18%): 1H NMR (300 MFIz, DMSO-J 6 ) δ 8.63 (d, 1 FI). 8.01 (d. 1 H). 7.93-7.83 (m. 2 H). 7.59- 7.44 (m. 4 H), 7.34-7.29 (m. 1 H). 6.25 (s. 1 H). 4.70-4.60 (m. 2 FI). 4.57-4.49 (m, 2 FI). 3.65-3.52 (m, 1 FI), 2.98-2.88 (m. 1 FI). 1.98-1.90 (in. 4 H). 1 .48 (d. 6 H). 1.42- 1.18 (m. 6 H); Cl MS m/z = 457 Example 63 - Preparation of Compound 78 To compound 24 (200 mg. 0.53 mmol) was added . ram- 1.4- diaminocyclohexane (2.00 g. 17 mmol) and EtOFI (4 mL). The reagents were heated in a sealed tube in an oil bath at 170 °C for 18 h. The mixture was cooled to 60 °C and partitioned between EtOAc and brine. The EtOAc layer was separated, washed with brine (3 x). dried with Na 2 S0 . and concentrated to give 78 (0.12 g. 50%): mp 135-138 °C: Η NMR (300 MHz. CDC1 ) δ 8.03-7.82 (m. 2 PI). 7.80-7.58 (m. 3 H). 7.57-7.30 (m. 3 PI). 7.30-7.05 (m. 1 FI). 6.20 (bs. 1 FI). 5.95-4.73 (m. 2 H). 4.73-4.45 (ni. 2 FI). 3.90-3.60 (m. 1 H), 2.80-2.52 (m. 1 H). 2.25-1 .80 (m. 4 FI). 1 .80-1.60 (bs. 3 FI). 1.52 (d. 6 FI). 1.38-1.05 (m. 4 H): IR (KBr) 3422. 2927. 1599. 1489. 1253. 779 cm " 1 : API MS m/z = 457 [C^P^Ns+Hf. Example 64 - Preparation of Compound 79 Compound 78 (50 mg. 0.1 1 mmol) was dissolved in CH2CL (2 mL) and stirred at room temperature. Pyridine (0.5 mL), Ac 2 0 (0.5 mL. 4.9 mmol). and DMAP (few crystals) were added to the reaction mixture and stirred for 2 h. The solution was diluted in CFLC1 2 and washed in 2N HC1. The HC1 layer was concentrated. CFLCL was added and the aqueous phase neutralized with saturated NaHC0 3 . The CFLCL layer was separated, dried (MgS0 ). and concentrated to give 79 (0.03 g. 55%) as a white solid: Η NMR (300 MFIz. CDC1 ) δ 8.00-7.80 (m. 2 H). 7.81-7.57 (m. 2 H). 7.56-7.33 (m. 3 H). 7.30-7.05 (m. 2 H). 6.15-5.90 (bs. 1 H). 5.47- 5.28 (m. 1 H). 4.96-4.72 (m. 2 H), 4.73-4.45 (m. 2 H). 2.25-1.82 (m, 4 H), 2.00 (s. 3 FI), 1.54 (d. 6 FI), 1.40-1.00 (m, 4 FI); API MS m/z = 499 [C 28 H 34 N 8 O+H] + . Example 65 - Preparation of Compound 80 Compound 74 (0.02 g, 0.05 mmol) was dissolved in dry benzene (5 mL) and stirred under a blanket of argon. The solution was cooled in an ice bath and pheny isocyanate (25 L. 0.23 mmol) was added dropwise. The ice bath yvas removed and the mixture stirred at room temperature for 0.5 h. The solvent was evaporated in vacuo to give a yellow oil. The crude product was purified by flash column chromatography on silica gel to give 80 (0.008 g): Η NMR (300 MFIz. CDCI 3 ) δ 7.53-7.30 (m. 10 H). 7.13-7.06 (m. 4 H). 6.98-6.88 (m. 1 H). 6.62 (s. 1 H). 6.02 (s. 1 FI). 5.65 (s. 1 H). 5.02 (d. 1 H). 4.85-4.70 (m. 2 H). 4.60-4.52 (m. 1 H). 4.45-4.40 (m. 1 H). 4.36-4.22 (m. 2 H). 4.00 (s. 1 FI). 1 .91 -1.60 (m. 6 H). 1.48-1.43 (m. 6 H). Example 66 - Preparation of Compound 82 A mixture of 6-chloronicotinamide (2.96 g. 18.9 mmol). phenylboronic acid (2.54 g. 20.8 mmol). and Pd(PPh 3 ) (643 mg. 0.565 mmol) in toluene (47 mL). ethanol (7 L) and 2M aqueous sodium carbonate solution (21 mL. 43 mmol) was stirred and heated at 90-100 °C under nitrogen for 16 h. The mixture was cooled to room temperature and filtered. The resulting solid was washed with water (2 x 20 mL) and dried in vacuo. To the dried solid was added methanol (50 mL). The mixture was stirred at reflux, cooled to room temperature, and filtered to give the product (90%) as a powder: mp 218-220 °C; Η NMR (500 Hz. DMSO-J 6 ) δ 9.23 (d. J = 2.5 Hz. 1 FI). 8.41 (dd. J, = 2.2 Hz. L = 8.3 Hz. 1 II), 8.32 (s. 1 H). 8.27 (d. J = 7.1 FIz. 2 H), 8.20 (d. J = 8.5 Hz. 1 H). 7.74 (s. 1 FI). 7.66-7.60 (m. 3 H). Example 67 - Preparation of Compound 83 To NaBFI 4 (0.19 g. 5 mmol) in 1.4-dioxane (4 mL) was added HOAc (0.3 g. 5 mmol) in 1 ,4-dioxane (2 mL) slowly while the flask was cooled with ice. Compound 82 (0.2 g, 1 mmol) was then added. The mixture was stirred at reflux at 100-1 10 °C for 4 h and the solvent was evaporated. To this mixture was added water (2 mL) slowly. The mixture was extracted with CH 2 C1 2 (4 x 10 mL). washed with water (3 x 5 mL). dried with anhydrous sodium sulfate. concentrated, and purified by flash chromatography on silica gel to provide the product as a yellow liquid. This was triturated with ethanol (1 mL) to provide a white solid which was collected (60%) and dried: mp 97-99 °C; Η NMR (500 FIz. CDC1 3 ) δ 8.60 (d. J = 2 FIz. 1 H), 7.97-7.95 (m, 2 H), 7.72-7.67 (ni. 2 H). 7.47-7.37 ( . 3 H). 3.90 (s. 2 FI). 1.77 (bs. 2 H). Example 68 - Preparation of Compound 84 A mixture of 2.6-dichloropurine (1. 0.19 g. 1 mmol). amine 83 (0.39 g, 2.15 mmol) in ethanol ( 13 mL). and water (3 mL) was heated at 100-1 10 °C under nitrogen for 24 h and then cooled to room temperature. The mixture was concentrated and water (5 mL) was added. A solid was filtered and washed w ith water (2 x 5 mL) and dried under vacuum to give the product (80%) as a \ ellow solid: nip 260 °C (dec): Η NMR (500 Hz. DMSO-J 6 ) δ 13.26 (s. 1 H). 8.79 (s. 1 H). 8.27 (s. 1 H). 8.16 (d. J = 7.1 Hz. 2 H). 8.34 (d. J = 7.3 Hz. 1 FI). 7.96 (d. J = 7.6 Hz. 1 FI). 7.63-7.52 (m. 3 H). 4.81 (s. 2 FI). Example 69 - Preparation of Compound 85 To a solution of compound 84 (0.34 g. 1 mmol) in DMSO (5 mL). was added potassium carbonate (0.7 g. 5 mmol) and 2-iodopropane (0.5 g. 3 mmol). The mixture was stirred at ambient temperature under nitrogen for 24 h and poured into ice water (30 mL). After filtration, the solid was washed with water (4 x 5 mL). dried under vacuum to give the crude product as a yellow solid. Flash column chromatography of the crude product on silica gel and recrystallization provided the pure product (63%) as ivory colored crystals: mp 138-139 °C; 1H NMR (500 Hz, CDC1 3 ) δ 8.70 (d, J =1.5 Hz. 1 H), 7.97 (m. 2 H). 7.79 (dd. J, = 1.7 Hz. J 2 = 8.1 Hz. 1 H). 7.71 (s. 1 H). 7.69 (d, J = 8.1 Hz. 1 H), 7.48-7.39 (m. 3 H), 4.87 (s. 2 H). 4.80 (m. 1 FI). 1.55 (d, J = 6.8 Hz, 6 H); CI MS m/z = 379 [C 20 HιgClN 6 +H] * . Anal. Calcd. for C 20 Hι 9 ClN 6 : C, 63.41 ; FI. 5.05; N, 22.18. Found: C. 63.75; FI, 5.09: N. 21.87. Example 70 - Preparation of Compound 86 To compound 85 (0.1 g, 0.26 mmol) was added tram- 1.4- diaminocyclohexane (1 g. 8.8 mmol) and EtOH (2 mL). The reaction mixture was heated in a sealed tube in an oil bath at 120 °C. The crude product was purified by column chromatography to give 86 (0.08 g. 67%): Η NMR (300 MHz, CDCI 3 ) δ 8.68 (d, 1 H), 7.83-7.97 (m. 2 FI), 7.70-7.83 (m. 1 FI), 7.55-7.73 (m. 1 H), 7.30-7.55 (m, 4 H), 6.35 (bs, 1 H). 4.72-4.95 (m. 2 H). 4.50-4.72 (m. 2 FI). 3.63-3.85 (m. 1 FI). 2.65-2.90 (m, 1 H), 2.37-2.63 (bs. 2 FI). 1.80-2.20 (dd. 4 H). 1.53 (d. 6 H), 0.72-1.42 (m. 4 H); API MS m/z = 457 [CofiHiiNs+Hf. Example 71 - Preparation of Compound 87 Compound 86 (0.08 g. 0.18 mmol) was stirred at room temperature in CH2CF (3 mL). Pyridine (100 mg. 0.82 mmol) was added followed b} Ac 2 0 (100 mg. 0.98 mmol ) and DMAP (few crystals). After 2 h. more CFLCL (3 mL) was added and the mixture was washed carefully with 2N HC1 (10 drops), and saturated NaHCO ,. After separation of the CH 2 CL layer, the organic phase was then dried with Na 2 S0 4 and concentrated to give 87 (80 mg. 92%): Η NMR (300 MFIz. CDC1 3 ) δ 8.72 (s. 1 H). 8.30-7.03 (m. 9 FI). 5.75-5.38 (m. 1 H). 5.02 (bs. 1 H). 4.83 (bs. 2 H). 4.72-4.40 (ni. 1 H). 3.73 (bs. 2 FI). 2.52-1.83 (m. 4 FI). 1.98 (s. 3 H). 1.52 (d. 6 FI). 1.50-1.00 (m. 4 FI); API MS m/z = 499 [C 28 H 3 N 8 O+H] " . Example 72 - Preparation of Compound 88 Compound 85 (0.05 g. 0.13 mmol) and (R)-(-)-2-amino-l -butanol (0.50 g. 5.6 mmol) were combined in a sealed tube and heated in an oil bath at 190 °C for 2 h then cooled to room temperature. The mixture was partitioned between EtOAc and brine, washed with brine (3 x). dried with Na2SO . and concentrated. The mixture was allowed to stand over the weekend and then purified by column chromatography on SiO 2 to give 88 (0.01 g. 17%) as a foam: Η NMR (300 MHz, CDCF,) δ 8.70 (s. 1 H). 8.05-7.82 (m, 2 H). 7.82-7.55 (m. 2 H). 7.57-7.30 (m, 4 H). 6.55 (bs. 1 H). 5.00-4.88 (s, 1 H). 4.78 (s. 2 H). 4.60 (heptuplet. 1 H). 3.98-3.83 (m. 1 H). 3.84-3.70 (ni. 1 FI), 3.70-3.50 (m. 1 FI), 2.90 (bs. 1 H), 1.75-1.55 (m. 2 H), 1.53 (d. 6 FI). 1.00 (t. 3 H); API MS m/z = 432 [C 2 H 29 N 7 O+H] + . Example 73 - Preparation of Compound 89 A mixture of 6-chloronicotinamide (2.5 g, 16 mmol). crude 2- trimethylstannylpyridine (5.8 g, 24 mmol), and PdCL(PPli3)2 (560 mg, 0.8 mmol) in DMF (35 mL) was heated at 150-160 °C in a pressure tube for 17 h. The DMF was distilled off under reduced pressure and the residue was extracted with ethyl acetate (6 x 30 mL) and concentrated. The residue was treated with methanol (15 mL) and a solid separated which was filtered and dried to give the product (40%>) as a powder: mp 237-240 °C: Η NMR (500 FIz. DMSO-J 6 ) 9.22 (d. J = 2.2 FIz. 1 H). 8.83 (m. 1 H) 8.57-8.53 (m. 2 FI). 8.48-8.46 (m. 1 H). 8.38 (s. 1 H). 8.1 1 -8.07 (ni. 1 H). 7.78 (s. 1 H). 7.63-7.60 (m. 1 H). Example 74 - Preparation of Compound 90 To NaBH (0.2 g. 5 mmol) in 1.4-dioxane (4 mL) was added HOAc (0.29 g. 5 mmol) in 1.4-dioxane (2 mL) slowly while the flask was cooled with ice. Compound 89 (0.199 g. 1 mmol) was then added. The mixture was stirred at reflux at 100-1 10 °C for 4 h and the solvent was evaporated. To this mixture was added water (2 mL) slowly. The mixture was extracted with CFLCL (4 x 10 mL). washed with water (3 x 5 mL), dried with anhydrous sodium sulfate. filtered, concentrated, and purified by flash chromatography on silica gel to provide the product as a yellow- liquid. This was triturated with ethanol ( 1 mL) and a white solid (32%) was collected and dried: mp 109-1 12 °C; Η NMR (500 Hz. CDC1 3 ) δ 8.63 (m. 1 H). 8.58 (s, 1 H). 8.32 (m. 2 H). 7.77 (m, 2 H), 7.25 (m. 1 H). 3.91 (s, 2 H). 1.94 (s. 2 H). Example 75 - Preparation of Compound 91 A mixture of 2.6-dichloropurine (1, 0.2 g, 1 mmol). compound 90 (0.4 g, 2.2 mmol) in ethanol ( 13 mL), and water (3 mL) was heated at 100-1 10 °C under nitrogen for 24 h and then cooled to room temperature. The mixture was concentrated and water (5 mL) was added. A solid was filtered and washed with water (2 x 5 mL) and dried under vacuum to give the product (83%) as a yellow solid: mp 248 °C (dec); Η NMR (500 Hz, DMSO-J 6 ) δ 13.27 (s. 1 FI). 8.81 (s. 1 H). 8.78 (d, J = 4.1 Hz, 1 H), 8.47 (m, 2 H). 8.28 (s, 1 H). 8.06-8.01 (m, 2 FI), 7.50 (m, 1 H), 4.84 (s. 2 H). Example 76 - Preparation of Compound 92 To the solution of compound 91 (0.35 g. 1 mmol) in DMSO (5 mL). added potassium carbonate (0.68 g. 5 mmol) and 2-iodopropane (0.49 g. 3 mmol). The mixture was stirred at ambient temperature under nitrogen for 24 h and poured into ice water (30 mL). After filtration, the solid was washed with water (4 x 5 mL). dried under vacuum to give the crude product as a yellow solid. Flash column chromatography of the crude product on silica gel and recrystallization provided the pure product (64%) as white crystals: mp 150-151 °C: Η NMR (500 Hz. CDC1 3 ) δ 8.71 (d. J = 1.9 Hz. 1 H). 8.67 (m. 1 H). 8.38-8.36 (m. 2 FI). 7.86-7.79 (ni. 2 H). 7.75 (s. 1 FI). 7.30 (m. 1 FI). 4.91 (s. 2 H). 4.82 (m. 1 FI). 1.57 (d. J = 6.8 Hz. 6 H): Cl MS m/z = 380 [CV.H|,sClN 7 +Pir. Anal. Calcd. for CιgH, 8 ClN 7 : C. 60.08: H. 4.78: N. -> 25.81 . Found: C. 59.76: H. 4.72; N. 25.57. Example 77 - Preparation of Compound 93 Compound 92 (150 mg. 0.39 mmol). .ram-1.4-diaminocyclohexane 0 (1.50 g. 13.1 mmol). and EtOFI (30 mL) were heated to 120 °C for 26 h in a sealed tube. The mixture was cooled, additional EtOAc was added, washed with brine, dried over Na 2 S0 . and concentrated to give 93 ( 170 mg. 94%) as a waxy solid: Η NMR (300 MHz. CDCI 3 ) δ 8.77-8.60 (m. 1 H). 8.44-8.27 (m. 2 H). 7.90-7.75 (ni. 2 H). 7.50 (s, 1 H). 7.36-7.22 (m, 2 FI), 6.27 (bs. 1 FI), 4.96-4.73 (m. 2 H), 4.73-4.52 (m, 2 H). 5 3.84-3.60 (ni. 1 PI). 2.80-2.57 (m. 1 FI). 2.22-2.00 (m. 2 H). 2.00-1.67 (m. 5 H). 1.54 (d, 6 FI), 1.38-1.05 (m, 4 FI); API MS m/r = 458 [C 25 H 3 iN 9 +H] + . Example 78 - Preparation of Compound 94 Compound 93 (0.15 g. 0.33 mmol) was dissolved in CH 2 CL (6 mL) and then pyridine (0.200 g, 1.64 mmol) followed by Ac 2 0 (0.200 g. 1.96 mmol) and DMAP (few crystals) were added. The reaction mixture was stirred for 2 h. washed with 2N FICl and NaHC0 . extracted with CH 2 C1 2 . dried with Na 2 S0 4 . and concentrated to give 94 (0.17 g. 69%) as a solid: mp 141-145 °C; Η NMR (300 MHz. CDCI 3 ) δ 8.80-8.63 (m. 1 H), 8.45-8.25 (t. 2 H), 7.95-7.73 (m. 1 H). 7.52 (s. 1 H), 7.35-7.20 (m. 2 H). 6.20 (bs. 1 FI), 5.50-5.30 (m. 1 FI). 4.98-4.75 (m. 2 H), 4.75- 4.50 (m, 2 FI). 3.84-3.60 (m. 2 FI). 2.27-1.87 (m. 4 FI). 2.00 (s. 3 H). 1.52 (d, 6 H). 1.40-1.10 (m. 4 H); API MS m/z = 499 [C 27 Fl 33 NgO+H] + . Example 79 - Preparation of Compound 95 DME (3 mL), tris(dibenzylideneacetone)dipalladium (0.01 g. 0.01 mmol). and PPh 3 (0.04 g, 0.15 mmol) were added to a round bottomed flask equipped with a condensor and maintained under an argon atmosphere. To the solution was added compound 11 (0.13 g. 0.25 mmol). 3-Fluorobenzene boronic acid (0.123 g. 0.9 mmol) was dissolved in a solution of 2M Na 2 CO 3 (0.6 mL) and DME ( 1 mL). and added to the reaction mixture. The mixture was stirred under argon and refluxed for 19 h then stirred at room temperature for 22 h. The reaction mixture was diluted with H 2 O. extracted with CH 2 CL. washed with brine. The organic layer was dried over Na 2 S0 and evaporated. The reaction mixture was purified twice by column chromatography and dried under high vacuum to give a white solid ( 17 mg. 14%): FI NMR (300 MFIz. CDCI 3 ) δ 7.56-7.32 (m. 8 FI). 7.08-6.99 (m. 1 H). 5.86 (br, 1 FI). 4.83 (d. 2 H). 4.71-4.56 (111. 1 H). 3.77 (br. 2 FI). 2.70 (br. 1 FI). 2.12 (d. 1 H). 1.88 (d. 1 FI). 1.51 (d. 6 FI). 1.22 (d. 5 H), 0.94-0.70 (m. 3 FI): API MS m/z = 474 Example 80 - Preparation of Compound 96 A stock solution of acetic anhydride was made by mixing CH 2 CL (16 mL). pyridine (4 mL). and Ac 2 O (0.16 mL). To this stock solution (1.5 mL) was added compound 95 (0.01 g. 0.02 mmol) followed by DMAP (few crystals). The reaction mixture was allowed to stir at room temperature for 26 h. The reaction mixture was then diluted with CH 2 C1 2 . washed with 2N HC1 until the aqueous layer was acidic, washed with NaHC0 3 . dried over MgS0 . evaporated, and dried in vacuo for 15 h to give a white solid (1 1 mg. 92%): Η NMR (300 MFIz. CDC1 3 ) δ 8.65 (br. 1 H). 7.77-7.17 (m. 8 FI). 7.1 1 -6.99 (m, 1 FI). 5.14 (br. 2 FI). 4.90 (br. 1 H). 4.69 (br, 1 FI). 3.78 (br. 2 H), 2.09 (br, 3 H), 1.94 (s, 2 H). 1.57 (d. 6 H). 1.42 (br, 4 H). 1.24 (s, 2 Pi). 0.94-0.76 (m, 1 H); Cl MS m/z = 516 [C 29 H 3 FN 7 O+H] + . Example 81 - Preparation of Compound 97 A stock solution of acetic anhydride was made by mixing CH 2 CL (16 mL). pyridine (4 mL). and AC 2 O (0.16 mL). To this stock solution (1.5 mL) was added compound 13 (0.01 g. 0.02 mmol) followed by DMAP (few crystals). The reaction mixture was allowed to stir at room temperature for 2 h. The reaction mixture was then diluted with CFLCL. washed with 2N HC1 until it was acidic, washed with NaHC03. dried over MgS0 . and evaporated to give a white solid (8 mg. 89%): Η NMR (300 MHz. CDCF) δ 8.78 (d. 1 H). 8.44 (t. 1 H). 7.95 (t. 2 H). 7.69- 7.45 (m. 5 H). 5.30 (br. 2 H). 4.84 (br. 1 H). 4.68 (br. 1 H). 3.78 (br. 2 FI). 2.39 (s. 3 H). 2.10 (br. 4 H). 1.96 (s. 2 FI). 1.57 (br. 10 H). 1.25 (s. 2 FI). 0.88 (br. 1 H): API MS .. -/_- = 512 [C 3 oH 3 -7N 7 O+H] ~ . Example 82 - Preparation of Compound 98 DME (3 mL). tris(dibeιιzylideneacetone)dipalladium (0.01 g. 0.01 mmol), and PPh 3 (0.04 g. 0.15 mmol) were added to a round bottom flask equipped with condensor and maintained under an argon atmosphere. Iodide 11 (0.13 g. 0.26 mmol). and 3-chlorobenzene boronic acid (0.15 g. 0.93 mmol) was dissolved in 2M Na 2 CO 3 (0.6 mL) and DME ( 1 mL). This was then added to the reaction mixture and refluxed for 19.5 h then stirred at room temperature for 30 h. The reaction mixture was then diluted with FLO. extracted with CFLCL. washed with brine, dried over Na 2 S0 . filtered, and evaporated. The reaction mixture was purified by column chromatography (3 x) and evaporated. The product was triturated in hexanes. filtered, and dried in vacuo for 1 h to give a white solid (16 mg): Η NMR (300 MHz, CDC1 3 ) δ 7.56-7.38 (m, 9 FI), 6.01 (br. 1 H). 4.80 (d, 2 H). 4.71-4.62 (m. 1 H). 3.77 (br. 2 H), 2.73 (br. 1 FI). 2.19-2.04 (m. 1 H), 1.94-1.85 (m. 1 H). 1.51 (d. 6 H). 1.24 (d. 5 H). 0.91 -1 .76 (m. 3 H); API MS .. i/z = 490 [C 27 H 3 2C1N 7 +H] * . Example 83 - Preparation of Compound 99 A stock solution of acetic anhydride was made by mixing CH 2 C1 2 (16 mL), pyridine (4 mL). and Ac O (0.16 mL). To this solution (1.5 mL) was added compound 98 (0.01 g. 0.02 mmol). followed by DMAP (few crystals). The reaction mixture was allowed to stir at room temperature for 2 h. The reaction mixture was diluted with CH 2 CL, washed with 2N FIG until the aqueous layer was acidic, washed with NaPIC0 3 . dried over MgSO , filtered, and evaporated to give a white solid (0.01 g. 83%): Η NMR (300 MFIz. CDCI 3 ) δ 7.65-7.35 (m. 8 H), 7.26-7.14 (m. 1 H). 5.23 (br, 1 FI), 4.66 (br, 1 FI). 3.78 (br. 2 FI). 2.18-2.00 (m. 4 FI). 1.94 (s. 3 H). 1.54 (d. 6 H), 1.24 (s. 5 FI). 0.94-0.69 (m. 3 IT); API MS m/z = 532 [C 29 H . 4 Cl N 7 O+Hf. Example 84 - Preparation of Compound 100 A stock solution of acetic anhydride was made by mixing CH 2 CL (16 mL). pyridine (4 mL). and Ac 2 0 (0.16 mL). To compound 14 (0.02 g. 0.03 mmol) was added this solution (2 mL). followed by DMAP (few crystals). The reaction mixture was allowed to stir at room temperature for 3 h. The reaction mixture was diluted with CH 2 CL. washed with 2N HC1 until the aqueous layer was acidic, washed with NaHCθ 3 . filtered, and evaporated to give a white solid (8 mg. 44%): Η NMR (300 MHz. CDCI 3 ) δ 7.41-7.32 (m. 7 FI). 7.26-7.14 (m. 1 FI). 5.96 (br. 1 H). 5.23 (d. 1 H). 4.84 (br. 2 H). 4.69-4.54 (m. 1 H). 3.75 (br. 1 FI). 2.21 -2.12 (m. 1 H). 2.09-1.96 (m. 1 H). 1.97 (s. 3 H). 1.54 (d, 6 H). 1.36-1.15 (m. 5 FI). 0.85 (br. 3 H); API MS m/z = 550 [C 29 H 3 3C1FN 7 0+Fl] t . Example 85 - Preparation of Compound 101 DME (3 mL). tris(dibenzylideneacetone)dipalladium (0.01 g, 0.01 mmol). and PPI1 3 (0.04 g, 0.15 mmol) were added to a round bottomed flask equipped with a condensor and maintained under an argon atmosphere. Compound 10 (0.13 g. 0.26 mmol) and 4-fluorobenzene boronic acid (0.13 g. 0.95 mmol) was dissolved in 2M Na 2 CO 3 (0.6 mL) and DME (1 mL). This was then added to the reaction mixture and refluxed for 19 h then stirred at room temperature for 72 h. The reaction mixture was then diluted with FLO. extracted with CH2CL. washed with brine, dried over Na 2 SO . filtered, and evaporated. The reaction mixture was purified by column chromatography on silica gel to give a white solid (17 mg. 14%): H NMR (300 MHz, CDCI 3 ) δ 7.56-7.38 (m, 8 H), 7.1 1 (t, 1 H), 5.81 (br. 1 H). 4.81 (d, 2 H). 4.69- 4.57 (m. 1 H). 3.78 (br. 2 PI). 2.69 (br. 1 H), 2.12 (br. 1 FI). 1.88 (br, 1 H), 1.54 (d. 6 H), 1.33-1.12 (m, 5 H), 0.85 (br, 3 H); API MS m/z = 474 [C 2 7H 3 2FN 7 +H] + . Example 86 - Preparation of Compound 102 A stock solution of acetic anhydride was made by mixing CH 2 CL (16 mL), pyridine (4 mL), and AC 2 O (0.16 mL). To the solution (1.4 mL) was added compound 101 (0.01 g, 0.02 mmol), followed by DMAP (few crystals). The reaction mixture was allowed to stir at room temperature for 2.5 h. The reaction mixture was diluted with CFLCL. washed with 2N PIG until the aqueous layer was acidic, and washed with saturated The organic layer was dried over MgS0 and evaporated to give a product (3 mg). The NaHCO 3 layer was further extracted with EtOAc (2 x). the organic layers were combined, dried over MgS0 . evaporated to give product 102 (2 mg). The products were combined using EtOAc. evaporated, and dried in vacuo for 15 h to give product 102 (5 mg. 50%): 'PI NMR (300 MHz. CDCF,) δ 7.71 -7.08 (m. 9 H). 5.29 (br. 2 FI). 4.84 (br. 1 H). 4.66 (br. 1 H). 3.78 (br. 2 H). 2.09 (br. 4 PI). 1.97 (s. 1 H). 1.57 (br. 3 H). 1.24 (d. 6 H). 0.87 (br. 5 FI): API MS «7/r = 516 [C2 9 H 34 FN 7 O+H] ' . Example 87 - Preparation of Compound 103 Compound 30 (0.10 g. 0.27 mmol) and .ra -1.4-diaminocyclohexane (0.48 g. 4.2 mmol) were combined with EtOFI (2 mL) in a sealed tube and heated at 190 °C for 24 h. and then stirred at room temperature for 46 h. The reaction mixture was purified by column chromatography and dried in vacuo to give 103 as a white solid (0.10 g. 81 %): 1H NMR (300 MHz. CDCI 3 ) δ 8.83 (d. 1 H). 8.58 (t. 1 H). 7.87- 7.83 (in. 1 PI). 7.55-7.47 (m. 5 H). 7.38-7.33 (m. 1 FI). 5.96 (br. 1 H). 4.82 (d. 2 FI). 4.68-4.59 (m. 1 PI). 3.75 (br. 2 PI). 2.69 (br. 1 H). 2.14 (d. 2 FI). 1.86 (d. 2 FI). 1.54 (d. 6 H). 1.31 -1.18 (m, 5 H); API MS m/z Example 88 - Preparation of Compound 104 A stock solution of acetic anhydride was made by mixing CH2CL (16 T mL), pyridine (4 mL). and AC 2 O (0.16 mL). To the solution (3.1 mL) was added compound 103 (0.02 g, 0.04 mmol). followed by DMAP (few crystals). The reaction mixture was allowed to stir at room temperature for 2.5 h. The reaction mixture was evaporated, dried in vacuo for 19 h. and purified by column chromatography to give a white solid (0.02 g): Η NMR (300 MHz. CDCF,) δ 8.83 (d. 1 IT). 8.59 (t. 1 H). 7.85 (d. 1 FI). 7.55-7.47 (in. 5 FI). 7.38-7.34 (m. 1 FI). 5.89 (br. 1 FI). 5.25 (d. 2 FI), 4.85 (br. 1 H), 4.66-4.61 (m. 1 H). 3.77 (br. 2 FI). 2.15 (br. 2 H). 2.05 (br. 2 FI). 1.97 (s. 2 IT). 1.54 (d. 6 PI). 1.33-1.25 (m. 5 11). 0.88 (br. 1 FI); API MS m/z = 499 [C 2 sFl 3 N 8 0+H] + . Example 89 - Preparation of Compound 106 Compound 72 (0.30 g. 0.80 mmol) and compound 105 ( 1 .15 g. 6.50 mmol) (Gardiner. J.M.. et al. Tetrahedron. 42(1 1 ):515 ( 1995). which is hereb} incorporated by reference, were combined with EtOFI (7 mL) and allowed to reflux for 23 h. Triethylamine ( 1 mL) was added and the reaction was refluxed further for another 21 h. The reaction mixture was then transferred to a sealed tube and EtOH (3 mL) was added. The reaction mixture was heated further at 100 °C for 3 h. The mixture was purified by column chromatograph}' to give 105 (0.13 g): Η NMR (300 MHz. CDG .) δ 7.57-7.26 (m. 10 H) 5.58 (br. 1 FI). 5.10 (br. 1 Pi). 4.83 (br. 1 H). 4.69-4.62 (m. 2 H). 3.36-2.91 (m. 5 11). 2.82-2.65 (m. 2 PI). 1 .53 (d. 2 H). 1 .44 (s. 9 FI). 1.25 (d. 1 FI). 1.13 (d. 3 PI): Cl MS m/z = 416 [C 2 «. H 39 N 7 O-Boc+H] " . Example 90 - Preparation of Compound 107 To compound 106 (0.10 g. 0.18 mmol) was added Et 2 O (2 mL). CFLCL ( 1 mL) and MeOH (1 mL). During 16 h HG/ether ( I M. 5 mL) was added while stirring. The resulting precipitate was collected by filtration and dried in vacuo for 30 min to provide 106 as an off-white solid (60 mg. 81 %): 1H NMR (300 MHz. DMSO) δ 8.48 (br. 2 H). 8.15 (br. 1 FI). 7.67-7.27 (m. 10 I). 4.79 (br. 1 FI). 3.60-3.42 (m. 3 I I). 3.18-3.06 (m. 2 H). 3.03-2.91 (m. 2 FI). 1.52 (d. 2 FI). 1 .27 (d. 6 PI); G MS /7//r = 41 6 [C 2 H 29 N 7 +H] + . Example 91 - Preparation of Compound 108 A stock solution of acetic anhydride was made by mixing CFLG 2 (16 mL). pyridine (4 mL), and AC 2 O (0.16 mL). To this solution (5.6 mL) was added compound 107 (0.04 g, 0.09 mmol), followed by DMAP (few crystals). The reaction mixture was allowed to stir at room temperature for 2 h. The reaction mixture was diluted with CH 2 CL. washed with 2N FIG until acidic, the organic layer was washed with NaHC0 3 . dried over MgS0 . filtered, and evaporated to give a white solid ( 16 mg). The product was purified by column chromatography to provide 108 as a white solid (0.01 g. 18%): Η NMR (300 MHz. CDCF) δ 7.58-7.43 (ni, 10 H). 6.60 (br. 1 H). 5.91 (br. 1 FI). 5.04 (t. 1 H). 4.84 (br. 2 H). 4.72-4.59 (m. 1 Pi). 4.10-4.02 (ni. 1 H). 3.59-3.47 (m. 2 FI). 1.80 (s. 3 PI). 1.57 (d. 6 PI). 1 .19 (d. 3 H); CI MS m/z = 458 [C 2 Fl , ιN ,0+H]\ Example 92 - Preparation of Compound 109 Compound 61 (1.0 g. 2.18 mmol). 3-chlorophenylboronic acid (1.3 g. 8.16 mmol). PPh . (0.3 g. 1.26 mmol). 2M Na 2 C0 3 (5.0 mL). and DME (54 mL) were added to a three-necked round-bottomed flask. The mixture was degassed w ith argon and heated to reflux for 40 min. cooled to room temperature, and then Pd 2 (dba) 3 (0.08 g. 0.08 mmol) was added. The reaction mixture was heated at reflux for 7 h. 3- Chlorophenylboronic acid (0.6 g) and Pd 2 (dba)-, (0.08 g) was then added and reflux continued for 12 h. The reaction mixture was cooled to room temperature, diluted with FLO (50 mL). and extracted with CFLCL (3 x 50 mL). The combined organic phases were washed with H 2 0 (50 mL). brine (50 mL). dried over Na S0 . filtered, and concentrated in vacuo. The residue was purified by silica gel chromatography and concentrated 7>7 vacuo to obtain compound 109 (950 mg. 89%): mp 178-181 °C; Η NMR (500 MFIz. CDC1 3 ) δ 7.56 (s. 1 H). 7.42-7.54 (m. 6 FI). 7.26-7.35 (m. 2 H). 6.08 (bs. 1 II). 4.81 (bs. 2 H). 4.59-4.64 (m. 2 II), 3.75-3.81 (m. 1 H). 2.65-2.72 (m, 1 H). 2.12 (d. 2 I I). 1.88 (d. 2 FI). 1.53 (d. 6 H). 1.18-1.27 (m. 4 FI): CI MS m/z = 490 Example 93 - Preparation of Compound 110 Compound 109 (500 mg, 1.02 mmol) was dissolved in anhydrous CFLCL (30 mL). cooled with an ice-water bath, followed by the addition of DMAP (12.2 mg. 0.1 mmol), pyridine (124 μL, 1 .53 mmol). and Ac 2 0 (106 μL. 1.12 mmol). The reaction mixture was stirred for 30 min at 0 °C an ice-water bath then stirred another 2 h at room temperature. The reaction mixture was then concentrated in vacuo and the residue was purified by column chromatography on silica gel. After removal of the solvent, the residue was dried in vacuo to give 110 (339 mg. 63%): mp 198-200 °C; Η NMR (500 MFIz. CDG ) δ 7.57 (s. 1 H). 7.39-7.53 (m. 6 H). 7.27-7.37 (m. 2 H). 6.31 (bs. 1 H). 5.28 (d. 1 FI), 4.78 (bs. 2 FI), 4.70 (d. 1 FI). 4.58- 4.67 (m. 1 PI). 3.72-3.83 (m. 1 11 ). 2.18 (d. 2 H). 2.00 (d. 2 H). 1.90 (s. 3 FI). 1.51 (d. 6 H). 1.18-1.31 (m. 4 H): CI MS m/z = 532 [C^TL G SO+HlJ Example 94 - Preparation of Compound 111 Compound 61 ( 1.0 g. 2.18 mmol). 2-thiopheneboronic acid (1.0 g. 8.16 mmol). PPh 3 (0.3 g. 1.26 mmol). 2M Na 2 C0 (5.0 mL). Pd 2 (dba) 3 (0.08 g. 0.08 mmol). and DME (54 mL) were added to a round-bottomed flask and purged with argon. The reaction mixture was heated at reflux for 24 h. 2-Thiopheneboronic acid (0.5 g). Pd 2 (dba) (0.1 g). and 2M Na C0 (2 mL) were added and heated to reflux for another 24 h. The reaction mixture was cooled to room temperature, diluted with H2O (50 mL) and extracted with CFLCL (3 x 50 mL). The organic phase was washed with H 2 0 (50 mL) and brine (50 mL). dried over Na 2 S0 . filtered, and concentrated in vacuo. The residue was repeatedly chromatographed on silica gel to obtain 111 (574 mg. 59%): mp 109-1 10 °C; Η NMR (500 MHz. CDCI 3 ) δ 7.56 (d. 2 H). 7.54 (s. 1 H). 7.46 (d. 2 H). 7.24-7.37 (m. 2 FI). 7.06 (t. 1 H). 6.04 (bs. 1 FI). 4.78 (bs. 2 H), 4.59-4.69 (m, 2 H). 3.75-3.81 (m, 1 H). 2.67-2.74 (m. 1 H). 2.14 (d. 2 H). 1.87 (d. 2 H). 1.52 (d, 6 H), 1.17- 1.29 (m. 4 FI); CI MS m/z = 462 [C2 5 H ιN 7 S+H] + . Example 95 - Preparation of Compound 112 Compound 111 (410.0 mg. 0.89 mmol) was dissolved in anhydrous CFLCL (30 mL) and purged with N 2 and cooled with an ice-water bath. Pyridine (108 mg. 1 .34 mmol) and DMAP (10.9 mg. 0.09 mmol) followed by Ac 2 O (92 μL. 0.98 mmol) were added slowly. The reaction mixture was stirred for 30 min in an ice- water bath followed by 2 h at room temperature. The reaction mixture was concentrated in vacuo. The residue was chromatographed on silica gel to give 112 (325 mg. 73%): mp 237-244 °C; Η NMR (500 MFIz. CDG 3 ) δ 7.54 (d, 2 H). 7.50 (s. 1 H). 7.36 (d, 2 H), 7.24-7.37 (111. 2 H). 7.08 (t. 1 FI). 6.06 (bs. 1 FI). 5.34 (s. 1 H). 4.78 (bs. 2 H). 4.58-4.70 (m. 2 FI). 3.78 (bs. 2 FI). 2.17 (d. 2 PI). 2.04 (d. 2 FI). 1.96 (s. 3 H). 1.56 (d. 6 FI). 1.18-1.32 (m. 4 FI); CI MS m/z = 504 [C-H .-^OS+P-f. Example 96 - Preparation of Compound 113 Compound 12 (600 mg. 1 .30 mmol) was dissolved in anhydrous GLCL (40 mL). purged with N 2 . and cooled to 0 °C followed b} an addition of DMAP ( 15.9 mg. 0.13 mmol). pyridine ( 165.3 mg. 1.95 mmol). and Ac 2 O ( 135 mg. 1 .43 mmol). The mixture was stirred 30 min at 0 °C then 2 h at room temperature. The reaction mixture was concentrated in vacuo. The residue was chromatographed on silica gel to give 113 (495 mg. 76%): mp 248-253 °C; Η NMR (500 MHz. CDCL) δ 7.54 (d. 2 H). 7.46 (s. 1 H). 7.35-7.41 (m. 5 FI). 6.13 (bs. 1 H). 5.28 (d. 1 H). 4.78 (br. 2 FI). 4.61 -4.63 (m. 2 H). 3.75 (bs. 2 FI). 2.14 (d. 2 FI). 1 .97 (d. 2 H). 1.95 (s. 3 H). 1.52 (d. 6 FI). 1.15-1.37 (m. 4 FI): CI MS m/z = 504 [C^FL . N-OS+Hf. Example 97 - Preparation of Compound 1 14 To compound 61 (1.0 g. 2.18 mmol) was added PPli 3 (330 mg. 1.26 mmol). 2M Na Cθ 3 (5 mL). DME (54 mL). and 4-carboxyphenylboronic acid ( 1.0 g. 6.03 mmol). The mixture was purged with N 2 for 45 min then Pd2(dba) 3 (366 mg. 0.4 mmol) was added and the mixture was heated at reflux for 3 d. The reaction mixture was diluted with FLO (100 mL). The aqueous layer was separated, and washed with CH 2 CL (3 x 40 mL). The aqueous layer was adjusted the pH to 5.8 by using IN HCl. Some precipitate appeared. The mixture was stored in a freezer overnight. The precipitate was collected and dried to obtain 114 (450 mg. 41 %): mp 246-249 °C (dec): Η NMR (500 MFIz. CD 3 OD+NaOD) δ 7.84 (s. 2 FI). 7.64 (s. 1 H). 7.54-7.63 (m. 4 H). 7.39 (s. 2 H), 6.08 (bs. 1 FI), 4.85 (bs, 2 FI). 4.73 (s. 1 H). 3.76 (m. 1 H). 2.74 (m, 1 FI). 1.99 (s. 2 FI). 1.88 (s. 2 H). 1 .63 (d. 6 PI). 1.21 -1.36 (m. 4 H): CI MS m/z = 500 [C 28 Fl 33 N 7 0 2 +H] + . Example 98 - Preparation of Compound 115 To a cooled MeOH (20 mL) solution was slow ly added TMSCI (253 μL. 2.0 mmol). The solution was stirred 20 min. followed by the addition of 114 (100 mg. 0.2 mmol). The reaction mixture was stirred at room temperature for 24 h. The reaction mixture was cooled with an ice-water bath then Et 3 N (557 mL) was added. The mixture was concentrated in vacuo. to provide the crude product, which was washed with water (2 x 20 mL ). The residue was purified b} chromatography on a silica gel. After removal of the solvent and drying in vacuo. the residue was dissolved in MeOFl (5 mL). followed by the addition of ether ( 10 mL). The precipitate was collected and dried to provide 115 (75 mg. 73%): mp 194-1 7 °C: Η NMR (500 MHz. CD 3 OD) δ 8.07 (d. 2 H ). 7.80 (s. 1 H). 7.72 (d. 2 FI). 7.63 (d. 2 H). 7.46 (d. 2 FI). 4.63-4.79 (m. 1 IT). 3.91 (s,. 3 H). 3.65-3.77 (m. 1 FI). 3.07 (bs. 1 H). 2.12 (d. 2 H). 2.01 (d. 2 FI). 1.55 (d. 6 H). 1 .29-1 .49 (111. 4 FI): API MS /z = 514 Example 99 - Preparation of Compound 117 To a suspension of compound 114 (250 mg. 0.50 mmol). pyridine (60 μL. 0.75 mmol). and DMAP ( 6.1 mg. 0.05 mmol) in H 2 0-dioxane (2: 1. 40 mL) was added AC 2 O (57 μL. 0.60 mmol ). After stirring 4 h at room temperature. K 2 CO (100 mg) was added followed by additional AC 2 O (100 μL). The reaction mixture was stirred 2 h at room temperature. Water (50 mL) was added and the pH was adjusted to 5. The precipitate was collected, washed with water and ether, and dried in vacuo. The precipitate (200 mg) was added to a solution of TMSCI (500 μL. 3.94 mmol) in MeOFl (25 niF). The reaction mixture was stirred 24 h at room temperature. The mixture was concentrated in vacuo. The product was purified by silical gel chromatography to provide 1 17 (145 mg. 52%): mp 247-250 °C; Η NMR (500 MHz, CDCF,) δ 8.09 (d. 2 FI). 7.64 (d. 2 H). 7.58 (d. 2 PI). 7.49 (s. 1 H). 7.45 (d, 2 PI), 5.91 (bs. 1 H). 5.18 (d. 1 11), 4.83 (bs. 2 IT). 4.61 -4.68 (m. 2 H). 3.93 (s. 3 H). 3.67-3.78 (ni, 2 F1). 3.07 (bs, 1 FI), 2.16 (d. 2 H). 2.02 (d. 2 PI). 1.95 (s, 3 H). 1.54 (d. 6 H). 1.23- 1.32 (m. 4 PI); API MS m/z = 556 [C 3 |H 37 N 7 0 3 +H " ] + . Example 100 - Preparation of Compound 116 To a solution of compound 117 (90 mg, 0.16 mmol) in MeOFI-FI 2 0 (6: 1. 23 mL) was added KOH ( 1 1 mg. 0.19 mmol) in 5 mL MeOFl. The reaction mixture was refluxed for 24 h. After removal of the solvent the residue was dissolved in 15 mL of water and washed with CFLCL. The aqueous layer was separated and adjusted pH to 4.5 by using IN HCl. The precipitate was collected and dried to obtain 116 (60 mg. 68%): mp 344-347 °C: Η NMR (500 MHz. DMSO-J 6 ) δ 1 1.21 (bs. 1 H). 8.14 (d. 2 H). 7.64-7.88 (m. 6 FI). 7.47 (d. 2 H). 6.06 (bs. 1 H). 5.18 (d. 1 I). 4.85 (bs. 2 H). 4.51-4.66 (m. 1 H). 3.62 (bs. 1 H). 3.46 (bs. 1 FI). 1.89 (bs. 2 H). 1.77 (bs. 5 H). 1.95 (s. 3 FI). 1 .47 (d. 6 H). 1.23-1.36 (m. 4 PI): API MS m/z = 542 Example 101 - Preparation of Compound 118 Compound 61 ( 1.0 g. 2.18 mmol). 3-carboxyphenylboronic acid ( 1 .0 g. 6.03 mmol). 2N Na 2 C03 (5 L). and DME/EtOPl (50 mL) were mixed together and degassed with N 2 for 1 h. Pd 2 (dba) 3 (366.0 mg. 0.4 mmol) and PPh (330.0 mg. 1.26 mmol) were added and the reaction mixture was heated to reflux for 48 h. The reaction mixture was cooled to room temperature, diluted with CH 2 CL (50 mL). and extracted with aqueous 5% Na 2 CO 3 (3 x 30 mL). The combined washes were extracted with CFLCL (3 x 30 mL) and ether (40 mL). The aqueous phase was neutralized to a pH of 5.8 using IN HCl and kept in a freezer for 1 h. The precipitate was collected, suspended in MeOH (30 n F) and the insolubles were removed by filtration. To the MeOFl solution was added ether (20 mL) to precipitate the product. The white solid was collected and dried in vacuo to offer 118 (65 mg, 6%): mp 205- 208 °C; Η NMR (500 MHz. CD 3 OD+NaOD) δ 8.17 (s. 1 FI), 7.88 (d. 1 H). 7.80 (s. 1 H). 7.56-7.63 (m. 3 PI). 7.35-7.41 (m. 3 PI). 6.08 (bs. 1 PI). 4.80 (bs, 2 H). 4.59-4.75 (m. 1 H), 3.72-3.82 (111. 1 FI). 2.89-3.01 (m, 1 II). 1.90-1.99 (m. 4 H). 1.51 (d. 6 H). 1.29-1 .40 (m. 2 FI). 1.12-1.23 (ni. 2 FI); API MS m/z = 500 [C 2S. H 33 N 7 O 2 +H] ' . Example 102 - Preparation of Compound 119 3-Thiopheneboronic acid (4.5 g, 35.2 mmol) and 6-chloronicotinamide (5.0 g. 32.0 mmol) were dissolved in DMA (150 mL). followed by the addition of 2N Na 2 CO (23 mL). N 2 gas was passed through the mixture for 1 h. Pd(PPh 3 ) (0.74 g, 0.64 mmol) was added and the reaction mixture was heated to reflux for 24 h. The reaction mixture was cooled to room temperature and poured into an ice-water (1 L) and stirred for 10 min. The precipitate was collected and washed with acetone. The collected solid was suspended in EtOAc (150 mL) and heated to reflux for 5 min. The solid was filtered and collected. After drying in vacuo, 119 (4.5 g. 69%) was - I l l - obtained: Η NMR (500 MHz. DMSO-. ) δ 9.08 (s. 1 FI). 8.34 (s. 1 PI). 8.28 (d. 1 H ). 8.20 (bs. 1 FI). 7.99 (d. 1 H). 7.81 (d. 1 FI). 7.71 (d. 1 H). 7.60 (bs. 1 PI). Example 103 - Preparation of Compound 120 To compound 119 (4.08 g. 20.0 mmol) suspended in THF (50 mL). was added I M BH ,-THF (164 mL). The mixture was heated to reflux for 9 h. The mixture was cooled with an ice-water bath and adjusted to a pFI of 1 -2. and stirred for 1 h at room temperature. The pH was adjusted to 9-10 (2N NaOFI) and extracted with EtOAc (3 x 50 mL). The combined organic phases were washed with H 2 0 (50 mL). brine (50 mL). and dried over Na 2 S0 . After filtration and removal of the solvent, the residue was dissolved in EtOFI (50 mL). followed by the addition of IM HG/ether (20 mL). The mixture was concentrated to dryness to provide 120 (2.03 g. 45%): Η NMR (500 MHz. CD OD) δ 8.93 (s. 1 H). 8.61 (d, 1 FI). 8.51 (s. 1 H). 8.43 (d. 1 H). 7.81 (d. 1 FI). 7.70 (d. 1 FI). 3.30 (t. 2 FI). Example 104 - Preparation of Compound 121 Compound 120 (2 g. 8.82 mmol). 2,6-dichloropurine (1.5 g. 8.01 mmol). EtOH (50 mL). and (.-Pr) 2 NEt (3.8 mL, 22 mmol) were heated at reflux for 16 h. The reaction mixture was then cooled with an ice-water bath. The precipitate was collected and washed with EtOPI. PLO. and ether. The precipitate was dried in vacuo to obtain 121 (0.84 g. 31%): Η NMR (500 MFIz. DMSO-J 6 ) δ 1 1.02 (bs. 1 H). 8.76 (bs. 1 H). 8.63 (s. 1 FI). 8.07 (bs. 2 FI). 7.79 (bs. 2 H). 7.71 (d. 1 PI). 7.64 (d. 1 H), 4.68 (bs. 2 FI). Example 105 - Preparation of Compound 122 Compound 121 (950 mg. 2.77 mmol) was dissolved in DMSO (50 mL). and then K 2 CO 3 (2.07 g. 15.0 mmol) was added, followed by the addition of 2- iodopropane (830 L. 8.31 mmol). The reaction mixture then was stirred at room temperature overnight. The reaction mixture was poured into an ice-water bath (400 mL). stirred for 10 min. and extracted with EtOAc (4 x 50 mL). The combined organic phases were washed with 1LO (40 mL). brine (40 mL). and dried over MgS0 . After filtration and removal of the solvent, the residue was dissolved in hot EtOAc (40 mL). followed by the addition of hexanes (80 mL). The precipitate was collected and dried in vacuo to obtain 122 (798 mg. 90%): Η NMR (500 MHz. CDG 3 ) δ 8.64 (s. 1 FI). 7.83 (s. 1 H). 7.70-7.79 (m. 2 H). 7.60 (d. 1 H). 7.55 (d. 1 H). 7.36 (d. 1 FI). 6.1 1 (bs. 1 H). 4.77-4.96 (m. 3 FI). 1.53 (d. 6 FI). Example 106 - Preparation of Compound 123 Compound 122 (780.0 mg. 2.03 mmol). .ra.?.v-1.4-diaminocyclohexane (2.3 g. 20.3 mmol). and EtOH (4 mL) were heated in a sealed tube to 150 °C for 20 h. The reaction mixture was poured into ice-water ( 150 mL) and stirred for 10 min. The resulting precipitate was washed with H 0 (2 x 20 mL ) and dried. The solid was chromatographed on a silica gel column. After removal of the solvent and drying in vacuo. 123 (765 mg) was obtained: mp 78-81 °C: Η NMR (500 MHz. CDG 3 ) δ 8.63 (s. 1 H). 7.87 (s. 1 FI). 7.72 (d. 1 H). 7.64 (d. 1 H). 7.55 (d. 1 PI). 7.04-7.09 (m. 1 H). 6.92 (s, 1 H). 5.95 (bs. 1 H). 4.64 (bs. 2 H). 4.33-4.45 (m. 2 FI). 3.74-3.77 (m. 1 H). 2.67-2.76 (m. 1 H). 2.13 (d. 2 FI). 1.90 (d. 2 H). 1 .63 (bs, 2 FI). 1.54 (d. 6 H). 1.19- 1.30 (m. 4 IT); 1 C NMR (CDG 3 ) δ 159.1. 155.0. 152.7. 151.3. 149.3. 143.3. 142.3, 136.2. 134.8. 133.4. 126.4. 126.4. 123.5. 120.2. 1 14.8. 50.4. 50.3. 46.5, 42.0, 35.7, 32.3. 22.8: API MS m/z = 463 [C 24 H 3 oN 8 S+H] + . Example 107 - Preparation of Compound 124 To an ice-cold solution of compound 123 (420 mg. 0.91 mmol) in CH 2 G 2 (20 mL) was added pyridine ( 1 10 μL. 1.4 mmol), DMAP ( 1 1.0 mg, 0.09 mmol) and Ac 2 0 (94.2 μL. 1 mmol). The reaction mixture was stirred for 30 min at 0 °C. followed by 2 h at room temperature. After removal of the solvent, the residue was chromatographed on a silica gel column. The resulting solid was recrystallized with EtOAc/MeOH and dried in vacuo to give 124 (350 mg. 79%): mp 249-252 °C; Η NMR (500 MHz. CDG 3 ) δ 8.61 (s. 1 FI), 7.85 (s. 1 H), 7.70 (d. 1 FI). 7.62 (d. 1 H). 7.53 (d. 1 PI). 7.48 (s. 1 FI), 7.38 (d, 1 FI). 6.00 (bs. 1 H). 5.25 (d. 1 H). 4.77 (bs. 2 FI). 4.53-4.72 (m. 2 H). 3.68-3.77 (m. 2 PI). 2.10 (d. 2 PI). 2.00 (d. 2 PI). 1 .94 (s. 3 H). 1.52 (d. 6 H). 1 .17-1.28 (m. 4 H); 13 C NMR (CDG 3 ) δ 169.4. 159.0. 155.0. 152.8. 149.2. 142.8. 142.3. 136.1. 134.9. 133.4. 126.5. 126.4. 123.5. 120.2. 1 14.9. 50.1. 48.3. 46.5. 42.2. 32.2. 32.1. 22.8: API MS m/z = 505 [C 26 H3 N s OS+H]J Example 108 - Description of Biological Assays A. Immunopurification of CyclinA/cdk2 and CyclinE/cdk2 Complexes. CyclinA/cdk2 and cyclinE/cdk2 assays were carried out with cyclin/cdk complexes isolated from HeFa S-3 suspension cultures. FleLa cells were grown in spinner flasks at 37 °C in Joklik ' s modified minimum essential media (MEM) supplemented with 7% horse serum. After growing in medium supplemented ith 2 mM thymidine for 16-1 8 h. cultures were arrested at the G l/S border and cyclinA/cdk2 and cyclinE/cdk2 were isolated from cell lysates by immunoprecipitation with antibodies specifically directed against each cyclin subunit. Rabbit anti-cyclinA (H-432) and the mouse monoclonal antibody against cyclinE (PIE1 1 1 ) were purchased from Santa Cruz Biotechnology. Cells blocked at the appropriate stage of the cell cycle were disrupted in lysis buffer (50 mM Tris. pH 8.0, 250 mM NaCl, 0.5% NP-40 plus protease and phosphatase inhibitors) and centrifuged at 10.000 x g to remove insoluble material. To isolate cyclin/cdk complexes. 1 μg of anti-cyclin antibody was incubated with lysate from 1 x 10 7 cells for 1 h at 4 °C. Protein A-coated agarose beads were then added for 1 h to collect antibody-bound immune complexes. The immobilized cyclin/cdk complexes were then washed 4 x with lysis buffer to reduce nonspecific protein binding. The complexes were then washed 1 x in kinase assay buffer (50 mM Tris-HCl, pH 7.4, 10 mM MgCL. 1 mM DTT) and aliquoted into individual assay tubes. B. Immunopurification of CyclinB/cdkl Complex. HeLa cells are blocked at the Gl/S border by culturing in the presence of 2 mM thymidine for 20 h. The cells are then rinsed 3x in phosphate buffered saline and resuspended in regular medium. After 4 h of culture, the mitotic blocker, nocodazole is added to a final concentration of 75 ng/ml. Sixteen hours later, the cells are harvested by centrifugation. washed in PBS. and lysed in cold Lysis Buffer (50 mM Tris pH 8.0. 250 mM NaCl. 0.5% NP-40. 1 mM DTT. 25 μg/ml leupeptin. 25 μ g/ml aprotinin. 15 μg/ml benzamidine. 1 mM PMSF. 50 mM sodium fluoride. 1 mM sodium orthovanadate) for 15 min at 1 x 10' cells/ml. The lysate is then clarified by centrifugation at 10.000 x g for 10 min. The supernatant is collected and diluted 1 :5 with Lysis Buffer. Monoclonal antibody against cyclinB (GNS1 ) is added to the supernatant to a final concentration of 5 μg/ml and shaken at 4 °C for 2 h. ' The immune complexes are then collected by the addition of 200 μl of protein agarose beads for 1 h. The beads are washed 4x in lysis buffer and lx in kinase assay buffer. C. Protein Kinase Assays and Determination of IC 50 Values. CyclinA/cdk2 assays were carried out with complexes isolated from 0.5 x 10 6 cells. CyclinE/cdk2 assays were carried out with complexes isolated from 4 x 10° cells. CyclinB/cdkl assays were carried out with complexes isolated from 4 x 10 4 cells. After centrifugation. the wash buffer was removed and the complexes resuspended in 15 μl of kinase assay buffer (kinase wash buffer ^ 167 μg/ml histone PH ). Compounds being tested for inhibition were added prior to the addition of [γ J" PJ ATP to a final concentration of 15 μM. The tubes were incubated at 30 °C for 5 min and the reactions were stopped by the addition of an equal volume of 2 x SDS- PAGE sample buffer. The samples were then subjected to electrophoresis on 10% SDS-PAGE to resolve the histone HI from other reaction components. The amount of radioactive phosphate transferred to histone HI was quantified on a Storm Phosphorimager (Molecular Dynamics). Prior to the protein kinase assay, test compounds were dissolved in DMSO at a concentration of 25 mM and were diluted to produce final concentrations of 0.1. 1.0. and 10.0 μM in the kinase assays. To eliminate possible effects of differences in DMSO concentration, the DMSO was kept constant at 0.04%. including the control reaction. Duplicate assays were performed at each concentration. The activity was plotted as the percent of activity in the absence of added test compound versus test compound concentration. IC50 values were calculated using GraphPad Prism data analysis software. D. Measuring the Inhibition of Cell Growth. Growth inhibition (GLo) values were measured with FleLa S-3 cells selected for growlh on plastic. The procedure was based on the protocol of Skehan et al. (Skehan. P., et al.. J. Natl. Cancer Inst.. 82: 1 107-1 1 12 (1990). which is hereby incorporated by reference) FleLa cells were plated at 2 x 10 cells/well in 96 well plates. One day later, a control plate was fixed by addition of TCA to 5%. After five rinses with tap water the plate was air dried and stored at 4 °C. Test compounds were added to the remaining plates at 10-fold dilutions between 0.01 and 100 μM. Two days later all plates were fixed as described above. Cells were then stained by the addition of 100 μl per well of 0.4% sulforhodamine B (SRB) in 1 % acetic acid for 30 min at 4 °C. Wells were then quickly rinsed 5 x with acetic acid ( 1 %) and allowed to air dry. The SRB was then solubilized by the addition of 100 μl per well of unbuffered 10 mM Tris base. Dye was quantified by measuring absorbance at 490 nm on a Molecular Devices kinetic microplate reader. Growth at each inhibitor concentration relative to the untreated control was calculated according to the following equation: percent growth = 100 x (T-T )/(C-T„). where T was the average optical density (OD) of the test wells after 2 days of treatment. T 0 was the average OD of the wells in the control plate on day 0 and C was the average OD of untreated wells. Plots of percent growth versus inhibitor concentration were used to determine the GIso. The data below shown in Table 2 summarizes the in vitro cyclin cdk inhibition constants (IC 50 ) and growth inhibition constants (GL 0 ) of HeLa Cells for the compounds of the current invention. Replicate experimental results are summarized below. Table 2: In Vitro Cyclin/cdk Inhibition (I o) and Growth Inhibition (GI ? o) of HeLa Cells For Compounds of the Current Invention. 17 The data below shown in Table 3 summarizes the in vitro cyclin cdk inhibition (IC 50 ) and growth inhibition (GLo) of HeLa Cells for several reference compounds in comparison to several compounds of the current invention. The chemical structures are provided. Table 3: In Vitro cyclin/cdk Inhibition (IC ? ) and Growth Inhibition (GI 5 ) of HeLa Cells For Reference Compounds in Comparison to Several Compounds of the Current Invention. The following data in Tables 4. 5. 6. and 7 summarize the growth inhibition properties of several compounds of the current invention and olomoucine against 60-human transformed cell lines. These data were cooperatively obtained at the National Cancer Institute in their 60-cell line growth inhibition assay according to published procedures (Boyd. M.R.. "Anticancer Drug Development Guide. " Preclinical Screening. Clinical Trials, and Approval \ 13 Teicher. B. Ed.: Humana Press: Totowa. NJ. 23-42 ( 1997). which is hereby incorporated b} reference). Table 4: In Vitro Growth Inhibition (GI 5() ) of NCI Human Transformed Cell Lines of Several Compounds of the Current Invention. Non-Small Cell HOP-92 1.00 1.58 — 0.32 Lung Non-Small Cell NCI-H226 0.22 0.11 — " Lung Non-Small Cell NCI-H23 0.32 0.16 26.3 0.32 Lung Non-Small Cell NC1-H322M 0.16 > 1000 38.9 0.40 Lung 1 Non-Small Cell NC1-H460 0.40 0.41 25.7 3.16 Lung Non-Small Cell NC1-H522 -- 4.2 " Lung Ovarian 1GROV1 0.32 0.20 10.0 0.16 Ovarian OVCAR-3 0.30 0.65 1000 1.00 Ovarian OVCAR-4 0.32 0.32 31.6 1.26 Ovarian OVCAR-5 0.25 0.26 > 1000 0.40 Ovarian OVCAR-8 __ 0.13 6.6 0.25 Ovarian SK-OV-3 0.95 0.40 > 1000 3.98 Prostate DU-145 7.08 0.63 17.8 1.26 Prostate PC-3 0.35 0.20 1000 0.40 Renal 786-0 0.20 0.25 18.6 0.32 Renal A498 2.88 1.58 - 1.26 Renal ACHN 0.32 0.40 5.2 2.00 Renal CAKl-1 1.66 0.13 4.4 0.20 Renal RXF 393 0.09 0.02 13.2 0.13 Renal SN12C - 0.56 - - Renal TK-10 " - 8.3 0.40 Renal UO-31 0.06 0.10 8.1 0.13 Table 5: In Vitro Growth Inhibition (GI 50 ) of NCI Human Transformed Cell Lines of Several Compounds of the Current Invention. Table 6: In Vitro Growth Inhibition (GIo) of NCI Human Transformed Cell Lines of Seλeral Compounds of the Current I ention. Table 7: In Vitro Growth Inhibition (GI ) of NCI Human Transformed Cell Lines of Several Compounds of the Current Invention and Olomoucine. Although the invention has been described in detail for the purpose of illustration, it is understood that such detail is solely for that purpose, and variations can be made therein by those skilled in the art without departing from the spirit and scope of the invention which is defined bv the following claims.

Claims: WHAT IS CLAIMED: A compound of the following formula: Formula I herein: R| are the same or different and independentl} selected from: H; C|-C -straight chain alkyl; C 3 -C -branched chain alkyl; X= N; CH; R 2 = phenyl; substituted phenyl. wherein the substituents ( 1 -2 in number) are in an} position and are independently selected from R|. OR| . SR|. S(0)R|. S(O 2 )R,. NHR,. NO,. OC(O)CH .. NHC(0)CH ,. F. Cl. Br. CF 3 . C(O)R, C(0)NHRι, phenyl, C(0)NHCHR,CH 2 OH; 1 -naphthyl; 2-naphthyl; heterocycles including: 2-pyridyl; 3-pyridyl; 4-pyridyl; 5-pyrimidyl; thiophene-2-yl: thiophene-3-yl: 2-furanyl; 3-furanyl: 2-benzofuranyl; benzothiophene-2-yl: 2-pyrrolyl; 3-pyrrolyl; 2-quinolinyl: 3-quinolinyl: 4-quinolinyl: 1-isoquinolinyl: 3-isoquinolinyl: 4-isoquinolinyl; substituted heterocycle. wherein the substituents (1-2 in number) are in any position and are independently selected from Br. Cl. F. R \ . C(O)CH : R, are the same or different and independent!}' selected from: PI; C|-C -straight chain alkyl; -C -branched chain alkyl: C 2 -C -alkenyl chain: (CII 2 ) n Ph: (CF1 2 ^-substituted phenyl. wherein the phenyl substituents are as defined above in R2: R-ι= H; C 1 -C -straight chain alkyl; C 3 -C -branched chain alkyl: R 3 and 1^ can be linked together by a carbon chain to form a 5-8-membered ring; n= 0-3; Y= H; OR,; NI-IR,; NHC(O)R.; NHC(O)NHR 3 : NHC(O)R..; NHC(0)OR 6 ; R..= C 3 -C 7 -cycloalkyl; Rβ= C|-C -straight chain alkyl: C ,-C 4 -branched chain alkyl: C 2 -C -alkenvl chain; (CII 2 )nPh: (CIl 2 )„-substituted phenyl. wherein the phenyl substituents are as defined above in R 2 : or a pharmaceutically acceptable salt thereof: with the proviso that when Rι=CH(CH ) 2 . and R 2 =Ph. and X=CH. then R≠H. and n≠O. and R≠H. and Y≠OH. 2. A compound according to claim 1 . wherein X - N. 3. A compound according to claim 1. wherein R 3 are the same or different and independently selected from: PI: C] -C -straight chain alkyl: C ,-C -branched chain alkyl; Y= H: OR, : NPIR, : NHC(O)R 3 : NHC(O)NHR : or a pharmaceutically acceptable salt thereof. 4. A compound according to claim 1 . wherein X= N: R 3 are the same or different and independently selected from: H; C ι-C -straight chain alkyl; C 3 -C -branched chain alkyl; Y= PI; OR, ; NHR, ; NHC(0)R ; NHC(0)NPIR 3 ; or a pharmaceutically acceptable salt thereof. 5. A compound according to claim 1 . wherein the compound has the following formula: Formula VII or a pharmaceutically acceptable salt thereof. 6. A compound according to claim 1. wherein the compound has the following formula: I' ormula XI or a pharmaceutically acceptable salt thereof. 7. A compound according to claim 1. wherein the compound has the following formula: Formula XII or a pharmaceutically acceptable salt thereof. 8. A compound according to claim 1. wherein the compound has the following formula: Formula XI II or a pharmaceutically acceptable salt thereof. 9. A compound according to claim 1. wherein the compound has the following formula: Formula XIV or a pharmaceutically acceptable salt thereof. 10. A compound according to claim 1. wherein the compound has the following formula: Formula X\ or a pharmaceutically acceptable salt thereof. 1 1. A compound according to claim 1. wherein the compound has the following formula: I- ormula Wl or a pharmaceutically acceptable salt thereof. 12. A compound according to claim 1. wherein the compound has the following formula: Formula XVII or a pharmaceutically acceptable salt thereof. 13. A compound according to claim 1. wherein the compound has the following formula: F ormula W ill or a pharmaceutically acceptable salt thereof. 14. A compound according to claim 1. wherein the compound has the following formula: F ormula XIX or a pharmaceutically acceptable salt thereof. 15. A compound according to claim 1. wherein the compound has the following formula: rmula XX or a pharmaceutically acceptable salt thereof. 16. A compound according to claim 1. wherein the compound has the following formula: Formula XXI or a pharmaceutically acceptable salt thereof. 17. A compound according to claim 1 , wherein the compound has the following formula: Formula XXII or a pharmaceutically acceptable salt thereof. 18. A compound according to claim 1. wherein the compound has the following formula: F ormula XXII I or a pharmaceutically acceptable salt thereof. 19. A compound according to claim 1. wherein the compound has the following formula: Formula XXIV or a pharmaceutically acceptable salt thereof. 20. A compound according to claim 1. wherein the compound has the following formula: ' , Formula XXVIII or a pharmaceutically acceptable salt thereof. 21. A compound according to claim 1. wherein the compound has the following formula: \l l rorniuL. \\\\ 1 or a pharmaceutically acceptable salt thereof 22. A compound according to claim 1 , wherein the compound has the following formula: F ormula XXXIX or a pharmaceutically acceptable salt thereof. 23. A compound according to claim 1. wherein the compound has the following formula: .38 Formula FII or a pharmaceutically acceptable salt thereof. 24. A compound according to claim 1. wherein the compound has the following formula: Formula I. II I or a phamiaceuticallv acceptable salt thereof. 25. A compound of the following formula: Formula III wherein: Ri are the same or different and independently selected from: PI: C|-C -straight chain alkyl: C 3 -C -branched chain alkyl: X= N: CH: R2= phenyl; substituted phenyl. wherein the substituents ( 1 -2 in number) are in any position and independently selected from R]. OR|. SRi . S(0)Rι. S(0 2 )Rι. NHR,. N0 2 . OC(0)CPP,. NHC(O)CH 3 . F. Cl. Br. CF . C(0)R,. C(0)NHR|. phenyl. QO.NHCHRiCH.OH; heterocycles including: 2-pyridyl; 3-pyridyl; 4-pyridyl: 5-pyrimidyl: thiophene-2-yl; thiophene-3-yl; 2-furanyl; 3 -f uranyl; 2-benzofuranyl; benzothiophene-2-yl; 2-pyrrolyl; 3-pyrrolyl; 2-quinolinyl; 3-quinolinyl; 4-quinolinyl; 1-isoquinolinyl: 3-isoquinolinyl; 4-isoquinolinyl; substituted heterocycle. wherein the substituents (1-2 in number) are in any position and are independently selected from Br, Cl, F, R|, C(0)CH 3 ; Y= OR, ; NHR, ; NHC(O)R,; NHSO 2 Rι; NHCJO)NHRι ; NPIC(0)OR (l : or a pharmaceutically acceptable salt thereof; Rd = C|-C -straight chain alkyl; CYC -branched chain alkyl; CVCi-alkenyl chain; or a pharmaceutically acceptable salt thereof. 26. A compound according to claim 25. wherein X=N. 27. A compound according to claim 25. wherein the compound has the following formula: NI L Formula VIII or a pharmaceutically acceptable salt thereof. 28. A compound according to claim 25. wherein the compound has the following formula: NH 2 Formula IX or a pharmaceutically acceptable salt thereof. 29. A compound according to claim 25. wherein the compound has the following formula: Ni l Formula X or a pharmaceutically acceptable salt thereof. 30. A compound according to claim 25. wherein the compound has the following formula: Formula XXV or a pharmaceutically acceptable salt thereof. 31. A compound according to claim 25. wherein the compound has the following formula: F ormula XX\ I or a pharmaceuticalK acceptable salt thereof. 32 A compound according to claim 25. wherein the compound has the following formula: l ormula WMI or a pharniaceutically acceptable salt thereof. 33. A compound according to claim 25, wherein the compound has the following formula: I ormul XIX or a pharmaceutically acceptable salt thereof. 34. A compound according to claim 25. wherein the compound has the following formula: Ni l, Formula XXX or a pharmaceutically acceptable salt thereof. 35. A compound according to claim 25. wherein the compound has the following formula: Formula XXXI or a pharmaceutically acceptable salt thereof. 36. A compound according to claim 25. wherein the compound has the following formula: OH Formula XXXII or a pharmaceutically acceptable salt thereof. 37. A compound according to claim 25. wherein the compound has the following formula: or a pharmaceutically acceptable salt thereof. 38. A compound according to claim 25. wherein the compound has the following formula: WO 00/55161 PCT/TJSOO/07065 - 145 - NH; Formula XX.\I\ or a pharmaceutically acceptable salt thereof. ;> 39. A compound according to claim 25. wherein the compound has the following formula: ^\ . ormula \\\\ or a pharmaceutically acceptable salt thereof. 40. A compound according to claim 25. wherein the compound has the following formula: Ni l; Formula \\X\ I I or a pharmaceuticalK acceptable salt thereof. 41. A compound according to claim 25. wherein the compound has the following formula: WW II! or a pharmaceutically acceptable salt thereof. 42. A compound according to claim 25. wherein the compound has the following formula: Ni l. Formula XI or a pharmaceutically acceptable salt thereof 43 A compound according to claim 25. wherein the compound has the following formula: V o "- Formula \l I or a pharmaceutically acceptable salt thereof. 44. A compound according to claim 25. wherein the compound has the following formula: M l; F ormula \I II or a pharmaceutically acceptable salt thereof. 45. A compound according to claim 25. wherein the compound has the following formula: . en , F orm ula XLIII or a pharmaceutically acceptable salt thereof. 46. A compound according to claim 25, wherein the compound has the following formula: HN . -CI I-, Formula XLIV or a pharmaceutically acceptable salt thereof. 47. A compound according to claim 25. wherein the compound has the following formula: Nil; Formula Xl.V or a pharmaceutically acceptable salt thereof. 48. A compound according to claim 25. wherein the compound has the following formula: Ml ll\^. Cll o . (irrnuli \1 \ I oi a phaimaceutically acceptable salt thereof 49 A compound accoiding to claim 25. wheiein the compound has the following formula -urmuli \l Ml or a pharmaceutically acceptable salt thereof 50 A compound according to claim 25, wherein the compound has the following formula or a pharmaceutically acceptable salt thereof 51 A compound according to claim 25. wherein the compound has the following formula: I or a pharmaceutically acceptable salt thereof. 52. A compound according to claim 25, wherein the compound has the following formula: ^^ Ml Nil; I-ormula I. or a pharmaceutically acceptable salt thereof. 53. A compound according to claim 25. wherein the compound has the following formula: UN CH~, T <) Formula I.I or a pharmaceutically acceptable salt thereof. 54. A compound according to claim 25. wherein the compound has the following formula: Nil; Formula LIY or a pharmaceutically acceptable salt thereof. 55 A compound according to claim 25. wherein the compound has the following formula: l l 11S ^_^ I I o _ ormui- I \ or a pharmaceutically acceptable salt thereof. 56. A compound according to claim 25. wherein the compound has the following formula: Ni l; F ormula 1 \ 1 or a pharmaceutically acceptable salt thereof. 57. A compound according to claim 25. yvherein the compound has the following formula: Formula IΛ I I or a pharmaceutically acceptable salt thereof. 58. A compound according to claim 25. wherein the compound has the following formula: UN ' CI 1-, T () Form ula IΛ ' II I or a pharmaceutically acceptable salt thereof. 59. A compound according to claim 25, wherein the compound has the following formula: CO.H N ;; > UN Ni l; 1 orniu 1 l\ or a pharmaceutically acceptable salt thereof. 60. A compound according to claim 25. wherein the compound has the following formula: Ni l; Formula l.X or a pharmaceutically acceptable salt thereof. 61. A compound according to claim 25. wherein the compound has the following formula: CU I I .-"^ SH M l . - CH Formula LXI or a pharmaceutically acceptable salt thereof 62 A compound according to claim 25. wherein the compound has the following formula: Formula LXII or a pharmaceutically acceptable salt thereof. 63. A compound according to claim 25. wherein the compound has the following formula: M l; Formula l.XIII or a pharmaceutically acceptable salt thereof. 64. A compound according to claim 25. wherein the compound has the following formula: NH ; Formula I.XI\ or a pharmaceutically acceptable salt thereof. 65. A compound according to claim 25. wherein the compound has the following formula: A " N us ~ N "V c " O . oi mula I \\ or a pharmaceutically acceptable salt thereof. 66. A method for inhibiting cell proliferation in mammals comprising: administering a therapeutically effective amount of the compound of claim 1 to the mammal. 67. The method of claim 66, wherein the compound is administered to a mammal suffering from a cell proliferation disorder selected from the group consisting of rheumatoid arthritis, lupus, type 1 diabetes, multiple sclerosis, cancer, restenosis, gout, and other proliferative diseases involving abnormal cellular proliferation. 68. The method of claim 67. wherein the cellular proliferation disorder is cancer. 69. The method of claim 67. wherein the cellular proliferation disorder is restenosis. 70. The method of claim 67. wherein the cellular proliferation disorder is type 1 diabetes. 71 . The method of claim 67, wherein the mammal is human. 72. A pharmaceutical composition of matter comprising the compound of claim 1 and one or more pharmaceutical excipients. 73. A method for inhibiting cell proliferation in mammals comprising: administering a therapeutically effect e amount of the compound of claim 25 to the mammal. 74. The method of claim 73. wherein the compound is administered to a mammal suffering from a cell proliferation disorder selected from the group consisting of rheumatoid arthritis, lupus, type 1 diabetes, multiple sclerosis, cancer, restenosis. gout, and other proliferativ e diseases invoK'ing abnormal cellular proliferation. 75. The method of claim 74. wherein the cellular proliferation disorder is cancer. 76. The method of claim 74. wherein the cellular proliferation disorder is restenosis. 77. The method of claim 74. wherein the cellular proliferation disorder is type 1 diabetes. 78. The method of claim 73, wherein the mammal is human. 79. A pharmaceutical composition of matter comprising the compound of claim 25 and one or more pharmaceutical excipients. 80. A process for preparation of a purine derivative compound of the formula: wherein: R, are the same or different and independently selected from: H: C ,-C -straight chain alkyl: C 3 -C -branched chain alkyl: X= N: CH; R = phenyl; substituted phenyl. wherein the substituents ( 1 -2 in number) are in any position and are independently selected from R, . OR, . SR| . S(0)R, . S(0 2 )R| . NHR, . N0 2 . OC(0)CH ,. NHC(0)CPP. F. Cl. Br. CF 3 . C(0)R,. C(0)NHR| . phenyl. C(0)NHCHR| CH 2 OH; 1 -naphthyl; 2-naphthyl; heterocycles including: 2-pyridyl; 3-pyridyl; 4-pyridyl; 5-pyrimidyl; thiophene-2-yl; thiophene-3-yl; 2-furanyl; 3-furanyl; 2-benzofuranyl; benzothiophene-2-yl; 2-pyrrolyl; 3-pyrrolyl; 2-quinolinyl; 3-quinolinyl; 4-quinolinyl; 1 -isoquinolinyl; 3-isoquinolinyl; 4-isoquinolinyl; substituted heterocycle, wherein the substituents (1 -2 in number) are in any position and are independently selected from Br. Cl. F, R| . C(O)CPI 3 ; R-, are the same or different and independently selected from: H; C ,-C -straight chain alkyl; G-G-branched chain alkyl; C 2 -C -alkenyl chain; (CI I 2 ),.Ph; (CPPJ n -substituted phenyl. wherein the phenyl substituents are as defined above in R^; R-,= H: C,-C -straight chain alkyl: C 3 -C -branched chain alkvl: R . and R_ 4 can be linked together by a carbon chain to form a 5-8-membered ring: n= 0-3; Y= H: OR, : NHR,; NHC(O)R 3 : NHSO2R3: NHC(O)NHR 3 NHC(O)R 5 : NHC(0)OR 6 : R 5 = C 3 -C -cycloalkyl; R 6 = C|-C -straight chain alkyl; C ,-C 4 -branched chain alkyl; CVC 4 -alkenvl chain; (ClI 2 )nPh; (CH 2 ) n -substituted phenyl. wherein the phenyl substituents are as defined above in R : or a pharmaceutically acceptable salt thereof; with the proviso that when Rι=CH(CH 3 ) 2 . and R =Ph. and X=CH. then R ≠H. and n≠O. and R_ι≠H. and Y≠OH. said process comprising: reacting a first intermediate compound of the formula: Formula IX where Z = Br or I with a compound of the formula: R -B(OH)2- R 2 -Sn(n-Bu) 3 . R 2 -Sn(Me) 3 . or mixtures thereof, under conditions effective to form the purine derivative compound. 81. A process according to claim 80 further comprising: reacting a second intermediate compound of the formula: with a second compound of the formula: under conditions effective to form the first intermediate compound. 82. A process according to claim 81. wherein if Y in the second compound is NH , said process further comprises: reacting the purine derivative compound with R 3 C(O)Cl or R 3 SO 2 CI or R 3 NCO or R OC(O)C l under conditions effective to form a final product having the same formula as the purine derivative compound except that Y is NPIC(0)R 3 or NHSO 2 R 3 or NHC(O)NHR 3 or NHC(0)OR 6 . A process according to claim 81 further comprising: reacting a third intermediate compound of the formula: Formula VI with a compound of the formula R,-Z under conditions effective to form the second intermediate compound. 84. A process according to claim 83 further comprising: reacting a first starting compound of the formula: F ormula IV with a second starting compound of the formula F ormula \ under conditions effective to form the third intermediate compound. 85 A process according to claim 80. wherein the purine derivative compound has the formula: Formula III 86. A process for preparation of a purine derivative compound of the formula wherein: R, are the same or different and independently selected from: H; C ,-C -straight chain alkyl; C ,-C -branched chain alkyl: X= N; CH; R = phenyl; substituted phenyl. wherein the substituents (1 -2 in number) are in any position and are independently selected from R| . OR, . SR, . S(0)R, . S(0 2 )R, . NHR, . N0 2 . OCJO)CH3. NHC(0)CH3. F. G. Br. CF 3 . C(0)R, . C(0)NHR, . phenyl. C(0)NHCHR l CH 2 OH; 1 -naphthyl; 2-naphthyl; heterocycles including: 2-pyridyl; 3-pyridyl; 4-pyridyl; 5-pyrimidyl: thiophene-2-yl; thiophene-3-yl; 2-furanyl; 3-furanyl; 2-benzof uranyl; benzothiophene-2-yl; 2-pyrrolyl; 3-pyrrolyl: 2-quinolinyl; 3-quinolinyl; 4-quinolinyl; 1 -isoquinolinyl; 3-isoquinolinyl; 4-isoquinolinyl; substituted heterocycle. wherein the substituents (1 -2 in number) are in any position and are independently selected from Br. CT. F. R| , C(0)CH 3 R 3 are the same or different and independently selected from: H; C|-C -straight chain alkyl; QrCrbranched chain alkyl; CVCi-alkenyl chain; (CH 2 )„Ph; (CPPVsubstituted phenyl. wherein the phenyl substituents are as defined above in R 2 ; C|-C -straight chain alkyl; C 3 -C -branched chain alkyl R ? and R_t can be linked together by a carbon chain to form a 5-8-membered ring: n= 0-3; Y= H: OR, : NHR,: NHC(O)R 3 : NHC(0)NHR 3 ; NHC(O)R 5 : NHC(0)OR 6 : R 5 = C 3 -C -cycloalkyl: R 6 = C,-C 4 -straight chain alkyl; C 3 -C -branched chain alkyl; C 2 -C -alkenyl chain; (CH 2 )„Ph: (CH 2 ) n -substituted phenyl, wherein the phenyl substituents are as defined above in R 2 ; or a pharmaceutically acceptable salt thereof; with the proviso that when Rι=CH(CH 3 ) 2 . and R 2 =Ph. and X=CPI, then R 3 ≠H. and n≠O, and R_ . ≠PI. and Y≠OH. said process comprising: reacting a first intermediate compound of the formula: F ormula XV11 with a compound of the formula: F ormula VIII under conditions effectiv e to form the purine derivative compound. 87. A process according to claim 86. wherein if Y in the second compound is NH 2 . said process further comprises: reacting the purine derivative compound with R 3 C(0)C1 or R 3 SO 2 CI or R 3 NCO or R 3 θC(0)C l under conditions effective to form a final product having the same formula as the purine derivative compound except that Y is NHC(O)R 3 or NHSO R3 or NHC(0)NHR or NHC(0)OR 6 . 88. A process according to claim 86 further comprising: reacting a third intermediate compound of the formula: Formula XVI with a compound of the formula R|-Z under conditions effective to form the second intermediate compound. 89. A process according to claim 88 further comprising: reacting a first starting compound of the formula: Formula IV with a second starting compound of the formula F ormula XV under conditions effective to form the third intermediate compound.

Inventors: TROVA MICHAEL P, TROVA, MICHAEL, P.
Applicants: ALBANY MOLECULAR RES INC, ALBANY MOLECULAR RESEARCH, INC.
Application year: 2000
Main classification: C07D473
IPC codes: A61K31, A61P3, A61P7, A61P19, A61P25, A61P35, A61P37, A61P43, C07D473