SUBSTITUTED 4-OXO-QUINOLINE-3-CARBOXAMIDES: GABA BRAIN RECEPTOR LIGANDS

Abstract: Disclosed are compounds of Formula (I) or the pharmaceutically acceptable salts thereof where, R1, R2, and W are defined herein. These compounds are highly selective agonists, antagonists or inverse agonists for GABAa brain receptors or prodrugs of agonists, antagonists or inverse agonists for GABAa brain receptors and are therefore useful in the diagnosis and treatment of anxiety, depression, Down Syndrome, sleep and seizure disorders, overdose with benzodiazepine drugs and for enhancement of memory. Pharmaceutical compositions, including packaged pharmaceutical compositions, are further provided. Compounds of the invention are also useful as probes for the localization of GABAA receptors in tissue samples.

Description: Substituted 4-oxo-quinoline-3-carboxamides : GABA Brain Receptor Li ands This application claims priority to provisional application no. 60/132,940, which is hereby incorporated by reference for the teachings described therein. Background of the Invention Field of the Invention This invention relates to 4-oxo-quinoline-3 - carboxamides and more specifically to such compounds that bind with high selectivity and high affinity to the benzodiazepine site of GABA A receptors. This invention also relates to pharmaceutical compositions comprising such compounds and to the use of such compounds in treatment of certain central nervous system (CNS) diseases. This invention also relates to the use of these imidazoloisoquinoline compounds in combination with one or more other CNS agents to potentiate the effects of the other CNS agents. Additionally this invention relates to the use such compounds as probes for the localization of GABA A receptors in tissue sections. Description of the Related Artt The GABA A receptor superfamily represents one of the classes of receptors through which the major inhibitory neurotransmitter , γ-aminobutyric acid, or GABA, acts. Widely, although unequally, distributed through the mammalian brain, GABA mediates many of its actions through a complex of proteins called the GABA A receptor, which causes alteration in chloride conductance and membrane polarization. A number of cDNAs for GABA A receptor subunits have been characterized. To date at least 6α, 3β, 3γ, lε, lδ and 2p subunits have been identified. It is generally accepted that native GABA A receptors are typically composed of 2α, 2β, and lγ subunits (Pritchett & Seeburg Science 1989; 245:1389- 1392 and Knight et . al . , i?ecept. Channels 1998; 6:1-18). Evidence such as message distribution, genome localization and biochemical study results suggest that the major naturally occurring receptor combinations are α 1 β 2 γ 2 , cc 2 β 3 γ 2 , α 3 β 3 γ 2 , and α 5 β 3 γ 2 ( ohler et . al . Neuroch. Res. 1995; 20(5): 631 - 636) . Benzodiazepmes exert their pharmacological actions by interacting with the benzodiazepine binding sites associated with the GABA A receptor. In addition to the benzodiazepine site, the GABA A receptor contains sites of interaction for several other classes of drugs. These include a steroid binding site, a picrotoxin site, and the barbiturate site. The benzodiazepine site of the GABA A receptor is a distinct site on the receptor complex that does not overlap with the site of interaction for GABA or for other classes of drugs that bind to the receptor (see, e.g., Cooper, et al . , The Biochemical Basis of Neuropharmacology, 6 th ed., 1991, pp. 145-148, Oxford University Press, New York). Early electrophysiological studies indicated that a major action of the benzodiazepmes was enhancement of GABAergic inhibition. Compounds that selectively bind to the benzodiazepine site and enhance the ability of GABA to open GABA A receptor channels are agonists of GABA receptors. Other compounds that interact with the same site but negatively modulate the action of GABA are called inverse agonists. Compounds belonging to a third class bind selectively to the benzodiazepine site and yet have little or no effect on GABA activity, but can block the action of GABA A receptor agonists or inverse agonists that act at this site. These compounds are referred to as antagonists. The important allosteric modulatory effects of drugs acting at the benzodiazepine site were recognized early and the distribution of activities at different receptor subtypes has been an area of intense pharmacological discovery. Agonists that act at the benzodiazepine site are known to exhibit anxiolytic, sedative, and hypnotic effects, while compounds that act as inverse agonists at this site elicit anxiogemc, cognition enhancing, and proconvulsant effects. While benzodiazepmes have a long history of pharmaceutical use as anxiolytics, these compounds often exhibit a number of unwanted side effects. These may include cognitive impairment, sedation, ataxia, potentiation of ethanol effects, and a tendency for tolerance and drug dependence . GABA A selective ligands may also act to potentiate the effects of certain other CNS active compounds. For example, there is evidence that selective serotonin reuptake inhibitors (SSRIs) may show greater antidepressant activity when when used m combination with GABA A selective ligands than when used alone. Summary of the Invention Disclosed are compounds, particularly 4-oxo- napthyrιdme-3-carboxamιdes, that bind to cell surface receptors. Compounds of the invention bind to GABA receptors, particular these compounds possess affinity for the benzodiazepine site of GABA A receptors, including human GABA A receptors . Preferred are compounds that exhibit high selectivity for the benzodiazepine site of the GABA A receptor. These compounds are therefore considered to be of use in the treatment of a broad array of diseases or disorders in patients which are characterized by modulation of GABA A receptors . Such diseases or disorders include, but are not limited to depression, anxiety, sleep disorders, cognitive disorders, low alertness, psychosis, obesity, pain, Parkinson's disease, Alzheimer's disease, neurodegenerative diseases, movement disorders, Down's syndrome, and benzodiazepine overdoses. Thus, the invention provides compounds of Formula I (shown below) , and pharmaceutical compositions comprising compounds of Formula I . The invention further comprises methods of treating patients suffering from certain CNS disorders with an effective amount of a compound of the invention. The patient may be a human or other mammal. Treatment of humans, domesticated companion animals (pet) or livestock animals suffering from certain CNS disorders with an effective amount of a compound of the invention is encompassed by the invention. In a separate aspect, the invention provides a method of potentiating the actions of other CNS active compounds. This method comprises administering an effective amount of a compound of the invention with another CNS active compound. Additionally this invention relates to the use of the compounds of the invention as probes for the localization of GABA A receptors in tissue sections. Accordingly, a broad aspect of the invention is directed to compounds of Formula I : Accordingly, a broad embodiment of the invention is directed to compounds of Formula I : Formula I or the pharmaceutically acceptable salts and solvates thereof, wherein R l f R 2 : and W are defined below, Detailed Description of the Invention The invention provides compounds of Formula I : Formula I or the pharmaceutically acceptable salts and solvates thereof, wherein: R and R ^ are the same or different and represent hydrogen, halogen, lower alkyl, lower alkoxy, lower alkoxyalkyl, or cycloalkyl or cycloalkyl alkoxy, where each cycloalkyl group has from 3-7 members, where up to two of the cycloalkyl members are optionally hetero atoms selected from oxygen and nitrogen, and where any member of the cycloalkyl group is optionally substituted with halogen, lower alkyl or lower alkoxy; and W is hydrogen; or W is cycloalkyl having from 3-7 members, where up to two of the members are optionally hetero atoms selected from oxygen and nitrogen, and where any member of the cycloalkyl group is optionally substituted with halogen, lower alkyl or lower alkoxy; or W is lower alkyl optionally substituted with up to three groups selected from: I) hydroxy, lower alkoxy, and halogen, li ) cycloalkyl having from 3-7 members, where up to two of the members are optionally hetero atoms selected from oxygen and nitrogen, and where any member of the cycloalkyl group is optionally substituted with halogen, lower alkyl or lower alkoxy, and m) aryl or heteroaryl optionally mono-, di-, or tri- substituted with: a 1 " halogen, hydroxy, lower alkyl, lower alkoxy, ammoalkyl, arylalkyl, heteroarylalkyl , b) -NR 5 R 6 wherein R 5 and R 6 are the same or 5 different and represent hydrogen, lower alkyl, or arylalkyl, c) - (CH 2 ) n O (CH 2 ) m R 7 wherein n and m are independently 0, 1, 2 or 3 and R 7 is lower alkoxy, aryl, heteroaryl, ammo, mono- or 10 dialkylammo, or cycloalkyl or cycloalkylalkoxy, where each cycloalkyl group has from 3-7 members, and where up to two of the cycloalkyl members are optionally hetero atoms selected from oxygen and nitrogen, or 15 d) -(CH 2 ) y NR 8 R 9 wherein y is 0, 1, 2 or 3 and R 8 and R 9 are the same or different and represent hydrogen, lower alkyl optionally substituted with trifluormethyl , aryl, heteroaryl, 20 arylalkyl, heteroarylalkyl, or cycloalkylalkyl , where the cycloalkyl portion has from 3-7 members, and where up to two of the members are optionally hetero atoms selected from oxygen and 25 nitrogen, or NR 8 R g together represent pipeπdmyl , 1,2,3, 4 -tetrahydroisoqumol yl , morpholmyl, 4-oxopιperιdmyl , or wherein R 10 is hydrogen, lower alkyl, acetyl or aryl, heteroaryl, arylalkyl, diarylalkyl or heteroarylalkyl, where each aryl portion is optionally substituted with up to three groups selected from halogen, hydroxy, lower alkyl, lower alkoxy, acetyl, or aryl. In addition, the present invention encompasses compounds of Formula II: Formula II wherein R x is as defined above for Formula I and R n is 1) hydroxy, lower alkoxy, halogen, 11) lower alkyl optionally mono- or disubstituted with lower alkoxy, m) cycloalkyl having from 3-7 members, where up to two of the members are optionally hetero atoms selected from oxygen and nitrogen, and where any member of the cycloalkyl group is optionally substituted with halogen, lower alkyl or lower alkoxy, or IV) aryl or heteroaryl optionally mono-, di-, or tπ- substituted with a) halogen, hydroxy, lower alkyl, lower alkoxy, heteroarylalkyl , b) -NR 5 R wherein R 5 and R 6 are the same or different and represent hydrogen, lower alkyl, or arylalkyl, c) - (CH 2 ) n O (CH 2 ) m R 7 wherein n and m are independently 0, 1, 2 or 3 and R 7 is lower alkoxy, aryl, heteroaryl, ammo, mono- or dialkylamino, or cycloalkyl or cycloalkylalkoxy, where each cycloalkyl group has from 3-7 members, and where up to two of the members are optionally hetero atoms selected from oxygen and nitrogen, or d) - (CH 2 ) y NR 8 R 9 wherein y is 0, 1, 2 or 3 and R 8 and R 9 are the same or different and represent hydrogen, lower alkyl optionally substituted with trifluormethyl , aryl, heteroaryl, arylalkyl, heteroarylalkyl, or cycloalkylalkyl , where the cycloalkyl portion has from 3-7 members, and where up to two of the members are optionally hetero atoms selected from oxygen and nitrogen, or NR 8 R g together represent piperidinyl, 1,2,3,4- tetrahydroisoqumolmyl , orpholinyl, 4- oxopipeπdmyl , or wherein R 10 is lower alkyl, acetyl or aryl, heteroaryl, arylalkyl, diarylalkyl or heteroarylalkyl, where each aryl portion is optionally substituted with up to three groups selected from halogen, hydroxy, lower alkyl, lower alkoxy, acetyl , or aryl . Preferred compounds of Formula II include those where R 1 is hydrogen, halogen, lower alkoxy, alkoxymethyl , or 1- morpholinyl and R X1 is (un) substituted lower alkyl, (un) substituted phenyl, 2- and 3 - (un) substituted thienyl, 2- and 3- (un) substituted furanyl , 2- and 3- (un) substituted tetrahydrofuranyl , or 2-, 3- and 4- (un) substituted pyπdyl . The present invention also encompasses compounds of Formula III : III wherein R x is as defined above in Formula I, n is 1, 2, 3 or 4 and X is CH 2 , oxygen or NR 12 , where R 12 is hydrogen or lower alkyl . Preferred compounds of Formula III include those where R λ is hydrogen or lower alkoxy and n is 2 or 3. Other preferred compounds of the invention are encompassed by the following formulae: Formula IV IV wherein R x is as defined above for Formula I; R 15 is hydrogen or lower alkyl; and R 12 R 12 , R 13 , R 14 , and R 14 are the same or different and represent a) hydrogen, halogen, hydroxy, lower alkyl, lower alkoxy, heteroarylalkyl; b) -NR 5 R 6 wherein R 5 and R 6 are the same or different and represent hydrogen, lower alkyl, or arylalkyl; c) - (CH 2 ) n O (CH 2 ) m R 7 wherein n and m are independently 0, 1, 2 or 3 and R 7 is lower alkoxy, aryl, heteroaryl, am o, mono- or dialkylamino, or cycloalkyl or cycloalkylalkoxy, where each cycloalkyl group has from 3-7 members, and where up to two of which members are optionally hetero atoms selected from oxygen and nitrogen, or wherein y is O, 1, 2 or 3 and R 8 and R 9 are the same or different and represent hydrogen, lower alkyl optionally substituted with tπfluormethyl, aryl, heteroaryl, arylalkyl, heteroarylalkyl, cycloalkylalkyl , where the cycloalkyl portion has from 3-7 members, and where up to two of which members are optionally hetero atoms selected from oxygen and nitrogen, or NR 8 R 9 together represent piperid yl , 1,2,3,4- tetrahydroisoqumol yl , morpholmyl, 4- oxopipeπdmyl , or wherein R 10 is lower alkyl, acetyl or aryl, heteroaryl, arylalkyl, diarylalkyl or heteroarylalkyl, where each aryl portion is optionally substituted with up to three groups selected from halogen, hydroxy, lower alkyl, lower alkoxy, acetyl, or aryl, provided that at least two of R 12 R 12 ., R 13 , R 14 , and R 14 , are hydrogen. More preferred compounds of Formula IV include those where R x is hydrogen, halogen, lower alkoxy, alkoxymethyl, or 1-morpholinyl , R 15 is hydorgen or methyl and R 12 is hydrogen. Formula V Formula V wherein R λ is as defined above in Formula I, d is l, 2, 3, 4, 5 or 6; R 14 is hydrogen, halogen, lower alkyl or lower alkoxy; A is C or N; B is CH, NH, S or 0; and C is S, CH 2 , CH, or N. More preferred compounds of Formula V include those where R 14 is hydrogen, R x is hydrogen, halogen, lower alkoxy, alkoxymethyl, or 1-morpholinyl and the ring containing A, B and C represents 2 -furanyl, 2 -tetrahydrofuranyl , 2- and 3- thienyl, or 1-imidazolyl . Formula VI Formula VI Wherein R x is as defined above in Formula I, R 14 is as defined above in Formula V, and A, B and C are independently CH or N, provided that no more than two are of A, B, and C are simultaneously N. More preferred compounds of Formula VI include those where R 14 is hydrogen; R λ is hydrogen, halogen, lower alkoxy, alkoxymethyl, or 1-morphol yl ; and the ring containing A, B and C represents 2- or 3-pyrιdmyl. Compounds of the invention can exist as tautomers solution. When structures and names are given for one tautomenc form the other tautomeπc form is also included m the invention. In certain situations, compounds of Formula I may contain one or more asymmetric carbon atoms, so that the compounds can exist different stereoisomeπc forms. These compounds can be, for example, racemates or optically active forms. In these situations, the single enantiomers, l. e., optically active forms, can be obtained by asymmetric synthesis or by resolution of the racemates. Resolution of the racemates can be accomplished, for example, by conventional methods such as crystallization m the presence of a resolving agent, or chromatography, using, for example a chiral HPLC column. Representative compounds of the present invention, which are encompassed by Formula I, include, but are not limited to the compounds Table I and their pharmaceutically acceptable acid and base addition salts. In addition, if the compound of the invention is obtained as an acid addition salt, the free base can be obtained by basifymg a solution of the acid salt. Conversely, if the product is a free base, an addition salt, particularly a pharmaceutically acceptable addition salt, may be produced by dissolving the free base in a suitable organic solvent and treating the solution with an acid, in accordance with conventional procedures for preparing acid addition salts from base compounds . Non-toxic pharmaceutical salts include salts of acids such as hydrochloric, phosphoric, hydrobromic, sulfuπc, sulfinic, formic, toluenesulfonic , methanesulfonic , nitic, bencoic, citric, tartaπc, maleic, hydroiodic, alkanoic such as acetic, HOOC- (CH 2 ) r -A c OOH where n is 0-4, and the like. Non-toxic pharmaceutical base addition salts include salts of bases such as sodium, potassium, calcium, ammonium, and the like. Those skilled m the art will recognize a wide variety of non-toxic pharmaceutically acceptable addition salts . The present invention also encompasses the acylated prodrugs of the compounds of Formula I . Those skilled m the are will recognize various synthetic methodologies which may be employed to prepare non-toxic pharmaceutically acceptable addition salts and acylated prodrugs of the compounds encompassed by Formula I . By "alkyl", "lower alkyl", and alkyl" m the present invention is meant straight or branched chain alkyl groups having 1-6 carbon atoms, such as, methyl, ethyl, propyl , isopropyl, n-butyl, sec-butyl, tert -butyl, pentyl , 2-pentyl, isopentyl, neopentyl , hexyl , 2-hexyl, 3-hexyl, and 3 -methylpentyl . By "alkoxy", "lower alkoxy", and "Cj-Cg alkoxy" m the present invention is meant straight or branched chain alkoxy groups having 1-6 carbon atoms, such as, for example, methoxy, ethoxy, propoxy, isopropoxy, n-butoxy, sec-butoxy, tert-butoxy, pentoxy, 2-pentyl, lsopentoxy, neopentoxy, hexoxy, 2-hexoxy, 3-hexoxy, and 3 -methylpentoxy . By the term "halogen" m the present invention is meant fluorine, bromine, chlorine, and iodine. By "cycloalkyl", e.g., C 3 -C 7 cycloalkyl, the present invention is meant cycloalkyl groups having 3-7 atoms such as, for example cyclopropyl, cyclobutyl, cyclopenyl, cyclohexyl, and cycloheptyl . In the C 3 -C 7 cycloalkyl groups, preferably m the C 5 -C 7 cycloalkyl groups, one or two of the carbon atoms forming the ring can optionally be replaced with a hetero atom, oxygen or nitrogen. Examples of such groups are piperidmyl, piperazmyl, morpholmyl, pyrrolidmyl , lmidazolidmyl , oxazolid yl , azaperhydroepmyl , oxazaperhydroepmyl , oxepanyl , oxazaperhydromyl , and oxadiazaperhydro yl . C and C 4 cycloalkyl groups having a member replaced by nitrogen or oxygen include aziridmyl, azetid yl, oxetanyl, and oxiranyl . By "heteroaryl" is meant one or more aromatic ring systems of 5-, 6-, or 7-membered rings containing at least one and up to four heteroatoms selected from nitrogen, oxygen, or sulfur. Such heteroaryl groups include, for example, thienyl, furanyl , thiazolyl, lmidazolyl , (is) oxazolyl , pyπdyl, pyrimidmyl , (iso) qumol yl , napthyπdmyl , benzimidazolyl , benzoxazolyl . Preferred heteroaryls are thiazolyl, pyrimidmyl, preferrably pyrιmιdm-2-yl, and pyridyl . Other preferred heteroaryl groups include 1-ιmιdazolyl , 2 -thienyl, 1-, or 2- qumolmyl, 1-, or 2- isoqumolmyl, 1-, or 2- tetrahydro isoqumolmyl, 2- or 3- turanyl and 2- tetrahydrofuranyl . By "aryl" is meant an aromatic carbocyclic group having a single ring (e.g., phenyl) , multiple rings (e.g., biphenyl) , or multiple condensed rings m which at least one is aromatic, (e.g., 1 , 2 , 3 , 4 -tetrahydronaphthyl , naphthyl, anthryl , or phenanthryl) , which is optionally mono-, di-, or trisubstituted with, e.g., halogen, lower alkyl, lower alkoxy, lower alkylthio, trifluoromethyl, lower acyloxy, aryl, heteroaryl, and hydroxy. Preferred aryl groups include phenyl and naphthyl, each of which is optionally substituted as defined herein. Representative compounds of the invention are shown below in Table 1. Table 1 10 The substituted 4-oxo-qumolme-3-carboxamιdes of the invention interact with a GABA binding site, the benzodiazepine (BDZ) receptor, as described m the examples. The compounds of Formula I and their salts are suitable for the diagnosis and treatment of anxiety, depression, memory impairment, Alzheimer's dementia, Down Syndrome, sleep, cognitive and seizure disorders, and overdose with benzodiazepine drugs and for enhancement of alertness, both m human and non-human animals including companion animals, e.g. domestic pets, especially dogs and cats and livestock animals, e.g. sheep, swine and cattle. The diseases and/or disorders that can be treated using compounds and compositions according to the invention include: Depression: depression, atypical depression, bipolar disorder, depressed phase of bipolar disorder. Anxiety: general anxiety disorder (GAD) , agoraphobia, panic disorder +/- agoraphobia, social phobia, specific phobia, Post traumatic stress disorder, obsessive compulsive disorder (OCD) , dysthymia, adjustment disorders with disturbance of mood and anxiety, separation anxiety disorder, anticipatory anxiety acute stress disorder, adjustment disorders, cyclopthymia Sleep Disorders : sleep disorders including primary insomnia, circadian rhythm sleep disorder, dyssomnia NOS, parasomnias, including nightmare disorder, sleep terror disorder, sleep disorders secondary to depression and/or anxiety or other mental disorders, substance induced sleep disorder Cognition impairment: cognition impairment, Alzheimer's disease, Parkinson's disease, mild cognitive impairment (MCI), age-related cognitive decline (ARCD) , stroke , traumatic brain injury, AIDS associate dementia, dementia associated with depression, anxiety or psychosis This invention provides compounds that bind with high affinity to the benzodiazepine site of GABA A receptors, including human GABA A receptors. This invention also provides compounds that bind with high selectivity to the benzodiazepine site of GABA A receptors, including human GABA A receptors . The invention also provides pharmaceutical compositions comprising compounds of the invention. The invention further comprises methods of treating patients m need of such treatment with an amount of a compound of the invention sufficient to alter the symptoms of a CNS disorder. Compounds of the inventions that act as agonists at 2 β 3 γ 2 an< 3 β 3 Y 2 receptor subtypes are useful in treating anxiety disorders such as panic disorder, obsessive compulsive disorder and generalized anxiety disorder; stress disorders including post-traumatic stress, and acute stress disorders. Compounds of the inventions that act as agonists at 2 β 3 γ 2 an d 3 β 3 β 2 receptor subtypes are also useful in treating depressive or bipolar disorders and in treating sleep disorders. Compounds of the invention that act as inverse agonists at the 5 β 3 γ 2 receptor subtype or ι β 2 Y ar ^d 5 β 3 γ 2 receptor subtypes are useful m treating cognitive disorders including those resulting from Down Syndrome, neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease, and stroke related dementia. Compounds of the invention that act as agonists at the x β 2 γ 2 receptor subtype are useful m treating convulsive disorders such as epilepsy. Compounds that act as antagonists at the benzodiazepine site are useful m reversing the effect of benzodiazepine overdose and m treating drug and alcohol addiction. In a separate aspect, the invention provides a method of potentiating the actions of other CNS active compounds, which comprises administering an effective amount of a compound of the invention m combination with another CNS active compound. Such CNS active compounds include, but are not limited to the following: for anxiety, serotonin receptor (e.g. 5-HT 1A ) agonists and antagonists; for anxiety and depression, neurokmm receptor antagonists or corticotropm releasing factor receptor (CRF X ) antagonists; for sleep disorders, melatonm receptor agonists; and for neurodegenerative disorders, such as Alzheimer's dementia, nicotimc agonists, muscarmic agents, acetylcholmesterase inhibitors and dopam e receptor agonists. Particularly the invention provides a method of potentiating the antidepressant activity of selective serotonin reuptake inhibitors (SSRIs) by administering an effective amount of a GABA agonist compound of the invention combination with an SSRI . Combination administration can be carried out in a fashion analogous to that disclosed Da-Rocha, et al . , J " . Psychopharmacology (1997) 11(3) 211-218; Smith, et al . , Am. J. Psychiatry (1998) 155(10) 1339-45; or Le , et al . , Alcohol and Alcoholism (1996) 31 Suppl . 127-132. Also see, the discussion of the use of the GABA A receptor ligand 3- (5- methylιsoxazol-3-yl) -6- (1-methyl-l, 2 , 3 -tπazol-4-yl) methyloxy-1, 2 , 4-trιazolo [3 , 4 -a] phthalz e m combination with nicotmic agonists, muscarmic agonists, and acetylcholmesterase inhibitors, m PCT International publications Nos. WO 99/47142, WO 99/47171, and WO 99/47131, respectively. Also see m this regard PCT International publication No. WO 99/37303 for its discussion of the use of a class of GABA A receptor ligands, 1 , 2 , 4 -tπazolo [4 , 3 - b] pyπdazmes , m combination with SSRIs. The present invention also pertains to methods of inhibiting the binding of benzodiazepine compounds, such as Rol5-1788, to the GABA A receptors which methods involve contacting a compound of the invention with cells expressing GABA A receptors, wherein the compound is present at a concentration sufficient to inhibit benzodiazepine binding to GABA A receptors m vi tro . This method includes inhibiting the binding of benzodiazepine compounds to GABA A receptors m vivo, e.g., a patient given an amount of a compound of Formula I that would be sufficient to inhibit the binding of benzodiazepine compounds to GABA A receptors m vi tro . In one embodiment, such methods are useful m treating benzodiazepine drug overdose. The amount of a compound that would be sufficient to inhibit the binding of a benzodiazepine compound to the GABA A receptor may be readily determined via a GABA A receptor binding assay, such as the assay described m Example 7. The GABA A receptors used to determine in vi tro binding may be obtained from a variety of sources, for example from preparations of rat cortex or from cells expressing cloned human GABA A receptors. The present invention also pertains to methods for altering the signal-transducmg activity, particularly the chloride ion conductance of GABA A receptors, said method comprising exposing cells expressing such receptors to an effective amount of a compound of the invention. This method includes altering the signal-transducmg activity of GABA A receptors m vivo , e.g., m a patient given an amount of a compound of Formula I that would be sufficient to alter the signal-transducmg activity of GABA A receptors m vi tro . The amount of a compound that would be sufficient to alter the signal-transducmg activity of GABA A receptors may be determined via a GABA A receptor signal transduction assay, such as the assay described m Example 8. The GABA A receptor ligands provided by this invention and labeled derivatives thereof are also useful as standards and reagents m determining the ability of a potential pharmaceutical to bind to the GABA A receptor. Labeled derivatives the GABA A receptor ligands provided by this invention are also useful as radiotracers for positron emission tomography (PET) imaging or for single photon emission computerized tomography (SPECT) . Non-toxic pharmaceutically acceptable salts include, but are not limited to salts with inorganic acids such as hydrochloπde, sulfate, phosphate, diphosphate, hydrobromide, and nitrite or salts with an organic acid such as malate, maleate, fumarate, tartrate, succmate, citrate, acetate, lactate, methanesulfonate, p-toluenesulfonate, 2- hydroxyethylsulfonate , salicylate and stearate . Similarly, pharmaceutically acceptable cations include, but are not limited to sodium, potassium, calcium, aluminum, lithium and ammonium. The present invention also encompasses the prodrugs of the compounds of Formula I . Those skilled in the art will recognize various synthetic methodologies that may be employed to prepare non-toxic pharmaceutically acceptable prodrugs of the compounds encompassed by Formula I. Those skilled in the art will recognize a wide variety of non-toxic pharmaceutically acceptable solvents that may be used to prepare solvates of the compounds of the invention, such as water, ethanol, mineral oil, vegetable oil, and dimethylsulfoxide . The compounds of general Formula I may be administered orally, topically, parenterally, by inhalation or spray or rectally in dosage unit formulations containing conventional non-toxic pharmaceutically acceptable carriers, adjuvants and vehicles. Oral administration in the form of a pill, capsule, elixir, syrup, lozenge, troche, or the like is particularly preferred. The term parenteral as used herein includes subcutaneous injections, intradermal, intravascular (e.g., intravenous), intramuscular, spinal, intrathecal injection or like injection or infusion techniques. In addition, there is provided a pharmaceutical formulation comprising a compound of general Formula I and a pharmaceutically acceptable carrier. One or more compounds of general Formula I may be present in association with one or more non-toxic pharmaceutically acceptable carriers and/or diluents and/or adjuvants and if desired other active ingredients. The pharmaceutical compositions containing compounds of general Formula I may be in a form suitable for oral use, for example, as tablets, troches, lozenges, aqueous or oily suspensions, dispersible powders or granules, emulsion, hard or soft capsules, or syrups or elixirs. Compositions intended for oral use may be prepared according to any method known to the art for the manufacture of pharmaceutical compositions and such compositions may contain one or more agents selected from the group consisting of sweetening agents, flavoring agents, coloring agents and preserving agents in order to provide pharmaceutically elegant and palatable preparations. Tablets contain the active ingredient in admixture with non- toxic pharmaceutically acceptable excipients that are suitable for the manufacture of tablets. These excipients may be for example, inert diluents, such as calcium carbonate, sodium carbonate, lactose, calcium phosphate or sodium phosphate; granulating and disintegrating agents, for example, corn starch, or algmic acid; binding agents, for example starch, gelatin or acacia, and lubricating agents, for example magnesium stearate, steaπc acid or talc. The tablets may be uncoated or they may be coated by known techniques to delay disintegration and absorption m the gastrointestinal tract and thereby provide a sustained action over a longer period. For example, a time delay material such as glyceryl monosterate or glyceryl distearate may be employed. Formulations for oral use may also be presented as hard gelatin capsules wherein the active ingredient is mixed with an inert solid diluent, for example, calcium carbonate, calcium phosphate or kaolin, or as soft gelatin capsules wherein the active ingredient is mixed with water or an oil medium, for example peanut oil, liquid paraffin or olive Aqueous suspensions contain the active materials in admixture with excipients suitable for the manufacture of aqueous suspensions. Such excipients are suspending agents, for example sodium carboxymethylcellulose, methylcellulose, hydropropylmethylcellulose, sodium algmate, polyvmylpyrrolidone, gum tragacanth and gum acacia; dispersing or wetting agents may be a naturally-occurring phosphatide, for example, lecithin, or condensation products of an alkylene oxide with fatty acids, for example polyoxyethylene stearate, or condensation products of ethylene oxide with long chain aliphatic alcohols, for example heptadecaethyleneoxycetanol , or condensation products of ethylene oxide with partial esters derived from fatty acids and a hexitol such as polyoxyethylene sorbitol monooleate, or condensation products of ethylene oxide with partial esters derived from fatty acids and hexitol anhydrides, for example polyethylene sorbitan monooleate. The aqueous suspensions may also contain one or more preservatives, for example ethyl, or n-propyl p- hydroxybenzoate, one or more coloring agents, one or more flavoring agents, and one or more sweetening agents, such as sucrose or saccharin. Oily suspensions may be formulated by suspending the active ingredients m a vegetable oil, for example arachis oil, olive oil, sesame oil or coconut oil, or m a mineral oil such as liquid paraffin. The oily suspensions may contain a thickening agent, for example beeswax, hard paraffin or cetyl alcohol . Sweetening agents such as those set forth above, and flavoring agents may be added to provide palatable oral preparations. These compositions may be preserved by the addition of an anti-oxidant such as ascorbic acid. Dispersible powders and granules suitable for preparation of an aqueous suspension by the addition of water provide the active ingredient m admixture with a dispersing or wetting agent, suspending agent and one or more preservatives. Suitable dispersing or wetting agents and suspending agents are exemplified by those already mentioned above. Additional excipients, for example sweetening, flavoring and coloring agents, may also be present . Pharmaceutical compositions of the invention may also be m the form of oil- -water emulsions. The oily phase may be a vegetable oil, for example olive oil or arachis oil, or a mineral oil, for example liquid paraffin or mixtures of these. Suitable emulsifying agents may be naturally-occurring gums, for example gum acacia or gum tragacanth, naturally-occurring phosphatides , for example soy bean, lecithin, and esters or partial esters derived from fatty acids and hexitol, anhydrides, for example sorbitan monoleate, and condensation products of the said partial esters with ethylene oxide, for example polyoxyethylene sorbitan monoleate. The emulsions may also contain sweetening and flavoring agents. Syrups and elixirs may be formulated with sweetening agents, for example glycerol , propylene glycol , sorbitol or sucrose. Such formulations may also contain a demulcent, a preservative and flavoring and coloring agents. The pharmaceutical compositions may be in the form of a sterile m ectable aqueous or oleaginous suspension. This suspension may be formulated according to the known art using those suitable dispersing or wetting agents and suspending agents which have been mentioned above. The sterile mjectable preparation may also be sterile mjectable solution or suspension in a non-toxic parentally acceptable diluent or solvent, for example as a solution in 1 , 3-butanedιol . Among the acceptable vehicles and solvents that may be employed are water, Ringer's solution and lsotomc sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose any bland fixed oil may be employed including synthetic mono- or diglycerides . In addition, fatty acids such as oleic acid find use in the preparation of mjectables. The compounds of general Formula I may also be administered in the form of suppositories, e.g., for rectal administration of the drug. These compositions can be prepared by mixing the drug with a suitable non- irritating excipient that is solid at ordinary temperatures but liquid at the rectal temperature and will therefore melt m the rectum to release the drug. Such materials are cocoa butter and polyethylene glycols. Compounds of general Formula I may be administered parenterally in a sterile medium. The drug, depending on the vehicle and concentration used, can either be suspended or dissolved m the vehicle. Advantageously, adjuvants such as local anesthetics, preservatives and buffering agents can be dissolved m the vehicle. Dosage levels of the order of from about 0.1 mg to about 140 mg per kilogram of body weight per day are useful m the treatment of the above- indicated conditions (about 0.5 mg to about 7 g per patient per day) . The amount of active ingredient that may be combined with the carrier materials to produce a single dosage form will vary depending upon the host treated and the particular mode of administration. Dosage unit forms will generally contain between from about 1 mg to about 500 mg of an active ingredient . Frequency of dosage may also vary depending on the compound used and the particular disease treated. However, for treatment of most disorders, a dosage regimen of 4 times daily or less is preferred. For the treatment of anxiety, depression, or cognitive impairment a dosage regimen of 1 or 2 times daily is particularly preferred. For the treatment of sleep disorders a single dose that rapidly reaches effective concentrations is desirable. It will be understood, however, that the specific dose level for any particular patient will depend upon a variety of factors including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, route of administration, and rate of excretion, drug combination and the severity of the particular disease undergoing therapy. For administration to non-human animals, the drug or a pharmaceutical composition containing the drug may also be added to the animal feed or drinking water. It will be convenient to formulate animal feed and drinking water products with a predetermined dose of the drug so that the animal takes m an appropriate quantity of the drug along with its diet. It will also be convenient to add a premix containing the drug to the feed or drinking water approximately immediately prior to consumption by the animal . Preferred compounds of the invention will have certain pharmacological properties. Such properties include, but are not limited to oral bioavailability, low toxicity, low serum protein binding and desirable in vi tro and m vivo half-lifes. Penetration of the blood brain barrier for compounds used to treat CNS disorders is necessary, while low brain levels of compounds used to treat periphereal disorders are often preferred. Assays may be used to predict these desirable pharmacological properties. Assays used to predict bioavailability include transport across human intestinal cell monolayers, including Caco-2 cell monolayers . Toxicity to cultured hepatocyctes may be used to predict compound toxicity. Penetration of the blood brain barrier of a compound in humans may be predicted from the brain levels of the compound m laboratory animals given the compound intravenously . Serum protein binding may be predicted from albumin binding assays. Such assays are described in a review by Oravcova, et al . (Journal of Chromatography B (1996) volume 677, pages 1-27) . Compound half -life is inversely proportional to the frequency of dosage of a compound. Tn vitro half-lifes of compounds may be predicted from assays of microsomal half- life as described by Kuhnz and Gieschen (Drug Metabolism and Disposition, (1998) volume 26, pages 1120-1127) . The present invention also pertains to packaged pharmaceutical compositions for treating disorders responsive to GABA A receptor modulation, e.g., treatment of anxiety, depression, sleep disorders or cognitive impairment by GABA A receptor modulation. The packaged pharmaceutical compositions include a container holding a therapeutically effective amount of at least one GABA A receptor modulator as described herein and instructions (e.g., labeling) indicating the contained GABA. receptor ligand is to be used for treating a disorder responsive to GABA A receptor modulation m the patient. An illustration of the preparation of compounds of the present invention is given m Scheme I . Scheme I In Scheme I, the substituents R and W carry the definitions set forth above for Formula I. As shown m Scheme I, an appropriate aniline is heated the presence of diethyl ethoxymethylenemalonate to afford the desired diethyl ammomethylene malonate, which is subsequently heated at temperatures above 200°C m a high boiling solvent such as, for example, phenyl ether to yield the corresponding ethyl-4-oxo-l , 4 -dιhydro-qumolme-3 - carboxylate. The ethyl ester is then saponified m an aqueous base such as IN NaOH and the resulting acid is then coupled to an appropriate amine under standard peptide coupling conditions. For example, the acid can be converted to an. activated ester with ethyl chloroformate m the presence of base . The disclosures this application of all articles and references, including patents, are incorporated herein by reference . The invention is illustrated further by the following examples, which are not to be construed as limiting the invention scope or spirit to the specific procedures described m them. Those having skill m the art will recognize that the starting materials, solvents and reaction conditions may be varied and additional steps employed to produce compounds encompassed by the present invention, as demonstrated by the following examples. Unless otherwise stated starting material and reagents employed m this synthesis are of standard commercial grade. In some cases, protection of certain reactive functionalities may be necessary to achieve some of the above transformations. In general, the need for protecting groups, as well as the conditions necessary to attach and remove such groups, will be apparent to those skilled the art of organic synthesis . The starting materials and various intermediates may be obtained f rom commercial sources , prepared from commercially available organic and/or inorganic sources , or prepared using well known synthetic methods . Representative examples of methods for preparing compounds of the invention are set forth below . Example 1 Preparation of starting materials and intermediates Representative examples of methods for preparing intermediates of the invention are set forth below. 1. Diethyl (4 -methoxyphenylaminomethylene) malonate A mixture of p-amsidine (2.20 g, 17.9 mmol) and diethyl ethoxymethylenemalonate 3.6 mL, 17.9 mmol) is heated at 130° C for 2 h. While warm, the mixture is evacuated, then cooled to give 5.18 g of diethyl (4- me hoxyphenylaminomethylene) malonate as an oil. 2. Ethyl 6-methoxy-4-oxo-l , 4-dihydro-quinoline-3 - carboxylate Diethyl (4 -methoxyphenylaminomethylene) malonate (5.18 g, 17.9 mmol) is added to phenyl ether (22 mL) preheated to 250° C. Heating is continued for 70 minutes. The reaction mixture is allowed to cool, diethyl ether is added, and the precipitate is collected, rinsed with diethyl ether and dried to afford 1.98 g of ethyl 6-methoxy-4-oxo-l, 4-dihydro- quinoline-3 -carboxylate . 3. 6-Methoxy-4-oxo-l , 4-dihvdro-σuinoline-3-carboxylic acid A mixture of ethyl 6-methoxy-4-oxo-l , 4-dihydro- quinoline-3 -carboxylate (1.22 g, 4.96 mmol), IN NaOH (25 mL) , and ethanol (5 mL) is heated at reflux for 1.5 h. The reaction mixture is cooled in an ice bath, acidified with aqueous HCl , and the precipitate is collected, rinsed with water and dried to give 0.95g of 6-methoxy-4-oxo-l , 4 - dιhydro-qumolme-3 -carboxylIC acid. Example 2 N-Tetrahvdrofurfuryl 4-oxo-l , 4-dιhydro-quιnol e-3 - carboxamide To a solution of 4-oxo-l , 4-dιhydro-qumolme- 3 - carboxylic acid (95 mg , 0.5 mmol) m a 4:1 mixture of THF:DMF (2.5 mL) and tπethylamme (146 μL, 1.05 mmol) at 0° C is added ethyl chloroformate (98 μL, 1.03 mmol) . The reaction mixture is allowed to stir for Ih before tetrahydrofurfurylam e (155 μL, 1.5 mmol) is added. The reaction mixture is stirred for 3/4h and then allowed to warm to ambient temperature for 2 Oh. The mixture is subsequently poured into aqueous ammonium chloride, the THF is removed in vacuo, and the mixture is extracted with ethyl acetate. The organic layer is dried (Na 2 S0 4 ) , filtered and concentrated. The residue is treated with IN NaOH (2 mL) and ethanol (0.5 mL) at reflux for lh. The reaction mixture is cooled, diluted with aqueous ammonium chloride and extracted 2X with dichloromethane. The combined organic layers are dried (Na 2 S0 4 ) , filtered, and concentrated to give 65 mg of N-tetrahydrofurfuryl 4-oxo-l , 4-dιhydro- quinolme-3 -carboxamide (compound 1) as a cream solid; m.p. 205-209° C. Example 3 N- [4 - Chloromethyl ) benzyl ] 4 -oxo- l , 4 -dιhvdro-quιnol ιne - 3 - carboxamide To a solut ion of N- [4 - (hydroxymethyl ) benzyl ] 4 -oxo- l , 4 - dιhydro-qumol me- 3 - carboxamιde ( 279mg ; 0 . 79mmol ) in anhydrous DMF (8mL) at 0° is added thionyl chloride (61μL; 0.83mmol) and the solution is stirred at room temperature for Ih. Water (lOmL) and brine (lOmL) are added, and the precipitate is collected, rinsed with a small quantity of methanol and ether, and dried to give 214 mg of N-[4- chloromethyl) benzyl] 4-oxo-l , 4-dihydro-quinoline-3 - carboxamide . N- { 4 - [ (N-Methyl -2 -phenethylamino) methyl] benzyl I 6 - ethoxy-4-oxo-l , 4 -dihydroguinoline-3 -carboxamide A mixture of N- [4 -chloromethyl) benzyl] 4-oxo-l, 4- dihydro-quinoline-3 -carboxamide (68mg; 0.183mmol), N- methylphenethylamine (67μL; 0.46MMOL), DMF (ImL), and water (0.2mL) is stirred at room temperature for 24h. The mixture is concentrated and the residue treated with water (5mL) and triturated with methanol. The solid is collected and rinsed with a small quantity of methanol and ether to give 38mg of N- {4- [ (N-methyl -2 -phenethylamino) methyl] benzyl} -6-ethoxy-4- oxo-1 , 4 -dihydroquinoline-3 -carboxamide (compound 2) . Example 4 The following compounds are prepared essentially according to the procedures described in Examples 1 -2 : (a) N- [2 - ( 2 -Hydroxyethoxy) ethyl ] 4 -oxo- l , 4 -dihydroquinoline- 3 - carboxamide ( compound 3 ) . (b) N- Isoamyl 4 -oxo- l , 4 -dihydro-quinoline- 3 - carboxamide; m.p. 180-181 C (compound 4) . (c) N-Benzyl 4-oxo-l , 4 -dihydro-quinoline-3- carboxamide; m.p. 173 C (compound 5) . (d) N- (2 -Fluorobenzy) 1 4-oxo-l , 4 -dihydro-quinoline-3 carboxamide; m.p. 238-242 C (compound 6). (e) N- (3 -Fluorobenzyl) 4-oxo-l , 4-dιhydro-qumolme-3 - carboxamide (compound 7) . (f) N- (4-Fluorobenzyl) 4-oxo-l , 4-dιhydro-qumolme-3 - o carboxamide; m.p. 213-215 C (compound 8) . (g) N- [ (2 -Furanyl ) methyl] 4-oxo-l , 4 -dihydro-qumol e- o 3 -carboxamide; m.p. 193-195 C (compound 9) . (h) N- (4-Methoxybenzyl) ] 4-oxo-l , 4-dιhydro-qumolme- o 3 -carboxamide ; m.p. 104-106 C (compound 10) . (l) N-Piperonyl 4-oxo-l , 4 -dihydro-qumolme- 3 - o carboxamide; m.p.207 C (compound 11) . ( ) N- (3 , 4-Dιmethoxybenzyl) 4-oxo-l , 4-dιhydro- o qumolme-3 -carboxamide ; m.p. 211-212 C (compound 12) . (k) N- [ (2 -Thienyl) methyl] 4-oxo-l , 4 -dihydro-qumolme - o 3 -carboxamide; m.p. 235-240 C (compound 13) . (1) N- [6- (2 , 3-Dιhydro-l, 4 -benzodioxmyl ) methyl] 4-oxo- o 1 , 4-dιhydro- qumolme-3 -carboxamide ; m.p. >350 C (compound 14) . (m) N- [ (2 -Fluoro-4-ethoxy) benzyl] 4-oxo-l , 4-dιhydro- o qumolme-3 -carboxamide ; m.p.179-181 C (compound 15) . (n) N- (4-Ethoxybenzyl) 4-oxo-l , 4-dιhydro-qumol e- 3- o carboxamide; m.p. 203-205 C (compound 16) . (o) N- (3-Ethoxypropyl) 4-oxo-l , 4 -dihydro-qumol e- 3 - o carboxamide; m.p. 141-142 C (compound 17) . (p) N- [ (2-Fluoro-4-ιsopropoxy) benzyl] 4-oxo-l, 4- o dihydro-qumolme- 3 -carboxamide; m.p. 108-110 C (compound 18) . (q) N- [4- ( Methyl ammomethyl) benzyl] 4-oxo-l , 4 -dihydro- qumolme- 3 -carboxamide hydrochloride (compound 19) . (r) N- [3- (1-Imιdazolyl) propyl] 4-oxo-l , 4 -dihydro- qumolme-3 -carboxamidehydrochloride (compound 20) . (s) N- [4- (1-Methylammoethyl) benzyl] 4-oxo-l, 4- dihydro-qu oline- 3 -carboxamide hydrochloride (compound 21) (t) N- [4- ( Ethyl am omethyl) benzyl] 4-oxo-l , 4-dιhydro- o qumolme-3 -carboxamide hydrochloride; m.p. 252 C (d) (compound 22 ) . (u) N- [4- ( 1-Imιdazolylmethyl ) benzyl] 4-oxo-l, 4- c dιhydro-qumolme-3 -carboxamide ; m.p. 215-218 C (compound 23) . (v) N- [4- (Methylam omethyl) benzyl] 6-chloro-4-oxo- 1 , 4 -dihydro-qumolme- 3 -carboxamide hydrochloride (compound 24) . (w) N- [4- (Ethylam omethyl) benzyl] 6-chloro-4 -oxo-1 , 4- dihydro-qumolme-3 -carboxamide (compound 25) (x) N- [4- (Dimethylammomethyl) benzyl] 6-chloro-4 -oxo- 1, 4 -dιhydro-qumolme-3 -carboxamide hydrochloride; m.p. 198 C (d) (compound 26) . (y) N- [3 - (Dimethylammomethyl) benzyl] 6-chloro-4-oxo- c 1 , 4-dιhydro- qumolme-3 -carboxamide hydrochloride; m.p. 178 C (d) (compound 27) . (z) N- [3- (Ethylammomethyl) benzyl] 6-chloro-4-oxo-l , 4- o dihydro-qumolme- 3 -carboxamide; m.p. 148 C (d) (compound 28) . (aa) N- [4- (1-Imιdazolylmethyl) benzyl] 6-chloro-4-oxo- 1 , 4 -dihydro-qumolme- 3 -carboxamide (compound 29) . (bb) N- [3 - (1-Imιdazolylmethyl ) benzyl] 6-chloro-4-oxo- 1 , 4 -dihydro-qumolme- 3 -carboxamide; (compound 30) . (cc) N-Benzyl 6-methoxymethyl-4-oxo-l , 4-dιhydro- qumolme- 3 -carboxamide; m.p. 195-196 C (compound 31) . (dd) N- [ (2 -Thienyl) methyl] 6-methoxymethyl-4-oxo-l , 4- o dihydro-qumolme -3 -carboxamide; m.p. 225-226 C (compound 32) . (ee) N- [ (3-Ethoxy) propyl] 6-methoxymethyl-4-oxo-l , 4- o dιhydro-qumolme-3 -carboxamide; m.p.95-97 C (compound 33) . (ff) N-Butyl 6-methoxymethyl-4-oxo-l , 4-dιhydro- qu ol me -3 -carboxamide; m.p. 157-159 C (compound 34) . (gg) N- [4- (Methylam omethyl) benzyl] 6-methoxymethyl-4 - o oxo-1 , 4 -dihydro-qumolme- 3 -carboxamide ; m.p. 249(d) C (compound 35) . (hh) N- [3 - (Methylammomethyl) benzyl] 6-methoxymethyl -4 - c oxo- 1 , 4 -dihydro-qumolme- 3 -carboxamide; m.p. 17(d) C ( compound 36) . (n) N-Butyl 6- (1-morpholmo) -4-oxo-l, 4-dιhydro- o qumolme-3 -carboxamide; m.p. 198-201 C (compound 37) . (JJ) N- [ (2-Thιenyl) methyl] 6- (1-morpholmo) -4 -oxo-1 , 4 - dihydro-qumolme- 3 -carboxamide; m.p. 268-269 C (compound 38) . (kk) N-Benzyl 6-ethoxy-4 -oxo-1 , 4 -dιhydro-qumolme-3 - o carboxamide; m.p. 209-211 C (compound 39) . (11) N- [ (4 -Ethoxy) benzyl] 6-ethoxy-4-oxo-l , 4-dιhydro- qumolme-3 -carboxamide; m.p. 201-204 C (compound 40) . (mm) N- [ (2 -Thienyl) methyl] 6-ethoxy-4-oxo-l , 4-dιhydro- qumolme-3 -carboxamide ; m.p. 223-224 C (compound 41) . (nn) N- [ (3 -Ethoxy) propyl] 6-ethoxy-4 -oxo-1, 4 -dihydro- qumolme- 3 -carboxamide; m.p. 179-181 C (compound 42) . (oo) N- [ (3 -Isopropoxy) propyl] 6-ethoxy-4-oxo-l , 4- dmydro-qumolme- 3 -carboxamide; m.p. 163-164 C (compound 43) . (pp) N- [ (2 -Tetrahydrof uranyl) methyl] 6-ethoxy-4 -oxo- o 1 , 4 -dihydro-qumolme -3 -carboxamide ; m.p. 181-182 C (compound 44) . (qq) N-Isoamyl 6-ethoxy-4-oxo-l , 4-dιhydro-qumolme-3 - o carboxamide; m.p. 180-183 C (compound 45) . (rr) N-Cyclohexyl 6-ethoxy-4 -oxo-1 , 4 -dihydro-qumolme- o 3 -carboxamide; m.p.191-193 C (compound 46) . (ss) N-Piperonyl 6-ethoxy-4 -oxo-1 , 4-dιhydro-qumolme- o 3 -carboxamide; m.p. 226-228 C (compound 47). (tt) N- [ (3 -Methoxy) propyl] 6-ethoxy-4-oxo-l , 4-dιhydro- o qumolme-3 -carboxamide; m.p. 175-177 C (compound 48) . (uu) N- (2-Fluorobenzyl) 6-ethoxy-4 -oxo-1 , 4 -dihydro- qumolme- 3 -carboxamide ; m.p. 237-238 C (compound 49) . (w) N- (3-Fluorobenzyl) 6-ethoxy-4-oxo-l , 4 -dihydro- qu ol me -3- carboxamide ; m.p. 210-212 C (compound 50) . (ww) N- [ (2-Pyrιdyl) methyl] 6-ethoxy-4-oxo- 1 , 4-dιhydro- qumolme-3 -carboxamide ; m.p. 221-222 C (compound 51) . (xx) N- [ (3 -Pyπdyl ) methyl] 6 -ethoxy- 4 -oxo-1, 4 -dihydro - qumolme-3 -carboxamide ; m.p. 222-224 C (compound 52) . (yy) N-Butyl 6-ethoxy-4-oxo-l , 4-dιhydro-qumolme-3 - carboxamide; m.p. 190-191 C (compound 53) . (zz) N-Pentyl 6-ethoxy-4-oxo-l , 4 -dihydro -qumol me -3 - carboxamide; m.p. 180-183 C (compound 54) . (aaa) N-Propyl 6-ethoxy-4-oxo-l , 4-dιhydro- 0 qumolme-3 -carboxamide; m.p. 219-221 C (compound 55) . (bbb) N- [ (2 -Methyl) propyl] 6 -ethoxy- 4 -oxo-1 , 4- dihydro- qumol me -3 -carboxamide; m.p. 214-216 C (compound 56) . (ccc) N-Hexyl 6 -ethoxy- 4- oxo-1 , 4-dιhydro- qumol me - 3 -carboxamide; m.p. 181-182 C (compound 57) . (ddd) N-Methyl 6 -ethoxy- 4 -oxo-1 , 4 -dihydro- qumolme -3 -carboxamide ; m.p. 279-281 C (compound 58) . (eee) N- [ (3 -Thienyl) methyl] 6 -ethoxy- 4 -oxo-1 , 4- dihydro -qumol me- 3 -carboxamide; m.p. 203-205 C (compound 59) . (fff) N- [4- (Methylammomethyl) benzyl] 6-ethoxy-4- oxo-1, 4-dιhydro-qumolme-3-carboxamιde hydrochloride; m.p. 295(d)° C (compound 60) . (ggg) N- [4- (Ethylammomethyl) benzyl] 6-ethoxy-4- oxo-1 , 4 -dihydro-qumolme- 3- carboxamide hydrochloride; m.p. 301(d)° C (compound 61) . (hhh) N- {4- [ (1 -Methyl) ammoethyl] benzyl} 6-ethoxy- 4 -oxo-1 , 4 -dihydro -qumol me -3 -carboxamide hydrochloride ; m.p. 271(d)° C (compound 62) . (m) N- [3- (Methylammomethyl) benzyl] 6-ethoxy-4- oxo-1 , 4 -dihydro-qumolme-3 -carboxamide hydrochloride; m.p. 217 (d)° C (compound 63) . (JJJ) N- {3- [ (1-Methyl) ammoethyl] benzyl} 6-ethoxy- 4 -oxo-1 , 4 -dihydro-qumolme-3 -carboxamide hydrochloride; m.p. 193(d) ° C (compound 64). (kkk) N- [3- [ (1-Imιdazolyl) methyl] benzyl} 6-ethoxy- 4 -oxo-1 , 4 -dihydro-qumolme- 3 -carboxamide ; (compound 65). (Ill) N- [4- [ (1-Imιdazolyl) methyl] benzyl} 6-ethoxy- o 4-oxo-l , 4-dιhydro-qumolme-3 -carboxamide; m.p. 161-165 C (compound 66) . (mmm) N-Benzyl 6 -methoxy-4 -oxo-1, 4 -dihydro- qumolme- 3 -carboxamide (compound 67) . (nnn) N- [4- (Methylammomethyl) benzyl] 6-methoxy-4- oxo-1 , 4 -dihydro -qumol e -3 -carboxamide hydrochloride (compound 68) . (ooo) N- [4- (Dimethylammomethyl) benzyl] 6-ethoxy-4- oxo-1 , 4 -dihydro -qumol me -3 -carboxamide hydrochloride; m.p. 222-225(d)° C (compound 69) . (ppp) N- [4- (Methylammomethyl) benzyl] 7-chloro-4- oxo-1 , 4 -dihydro-qumol e-3 -carboxamide hydrochloride (compound 70) . (qqq) N-Benzyl 8-fluoro-4-oxo-l , 4-dιhydro- qumolme-3 -carboxamide hydrochloride; m.p. 235-237 C (compound 71) . (rrr) N-Butyl 8-fluoro-4 -oxo-1 , 4-dιhydro-qumol e- o 3 -carboxamide hydrochloride; m.p. 205-207 C (compound 72) . (sss) N-4-Fluorobenzyl 6-chloro-4-oxo-l , 4- dihydroqumoline-3 -carboxamide ; m.p. 273-276°C (compound 73) . (ttt) N- { [4- [2- (1-Cyclohexyl) ethoxy] methyl] benzyl } 6-ethoxy-4-oxo-l, 4 -dihydroqumoline- 3 -carboxamide ; m.p . 171-173°C (compound 74) . (uuu) N- {4- [1- (2-Pyrιdyl)methoxy] methyl] benzyl} 6 -ethoxy-4 -oxo-1 , 4 -dihydroqumolme- 3 -carboxamide ; m.p. 125-127°C (compound 75) . (vw) N- {4- [1- [4- (2- Qumol ylmethyl) piperazmyl] methyl] }benzyl} 6-ethoxy-4 -oxo- 1 , 4 -dihydroqumolme-3 -carboxamide (compound 76). (www) N- {4- [1- [4- (4- Chlorobenzhydryl) piperazmyl] methyl] benzyl ) 6-ethoxy-4-oxo- 1 , 4 -dihydroqumolme-3 -carboxamide; m.p. 126-129°C (compound 77) . (xxx) N-{4- [1- [4- (4- Acetylphenyl) piperazmyl] methyl] benzyl } 6-ethoxy-4-oxo-l , 4- dihydroqu olme- 3 -carboxamide; m.p. 213-215°C (compound 78) . (yyy) N- { [4- [1- (4-Pyridyl) methoxy] methyl] benzyl} 6 -ethoxy-4 -oxo-1,4-dihydroqumolme- 3 -carboxamide ; wax (compound 79) . (zzz) N{4- [1- [4- (4- Biphenylmethyl) piperazmyl] methyl] benzyl} 6-ethoxy-4-oxo- 1 , 4 -dihydroqumol me- 3 -carboxamide; m.p. 207-210°C (compound 80) . (aaaa) N- {4- [1- (4-Oxopιpeπdmyl) methyl] benzyl } 6-ethoxy-4-oxo-l , 4 -dihydroqumol me -3 -carboxamide (compound 81) . (bbbb) N- [4- (Dibenzylammomethyl) benzyl] 6-ethoxy-4- o oxo-1 , 4-dιhydro-qumolme-3 -carboxamide ; m.p. 162-164 C (compound 82) . (cccc) N-{4- [ [2- (4- Morpholmyl) ethyl] ammomethyl] benzyl} 6 -ethoxy- 4 -oxo-1 , 4- o dihydro-qumolme- 3 -carboxamide; m.p. 123-127 C (compound 83) . (dddd) N-{4-[[3-(4- Morpholmyl) propyl] ammomethyl] benzyl} 6-ethoxy-4-oxo- 1 , 4- dihydro-qumolme-3 -carboxamide; wax (compound 84) . (eeee) N-{4-[[2- (Diisopropylammo) ethoxy] methyl] benzyl } 6 -ethoxy-4 -oxo-1 , 4- dιhydro-qumolme-3 -carboxamide; wax (compound 85) . (ffff) N-{4- [ [2- (4-Morpholmyl) ethoxy] methyl] benzyl } 6 -ethoxy-4 -oxo-1, 4 -dihydro-qumolme-3 -carboxamide ; wax (compound 86) . (gggg) N-{4- [ [ (2- (ι- Pipeπdmyl ) ethoxy] methyl] benzyl } 6-ethoxy-4-oxo-l , 4- dihydro-qumolme-3 -carboxamide; wax (compound 87) . (hhhh) N-{4-[[2-(l- Pyrrolid yl) ethoxy] methyl] benzyl} -6-ethoxy-4-oxo-l , 4- dihydro-qumolme-3 -carboxamide;wax (compound 88) . (mi) N- {4- [ (4 -Morpholmy) methyl] benzyl} 6 -ethoxy- o 4-oxo-l , 4 -dihydro-qumolme-3 -carboxamide; m.p. 168-170 C (compound 89) . (Dili) N-{ (4- [[1- [4-(2- Methoxyphenyl) piperazmyl] methyl] benzyl] 6 -ethoxy-4-oxo- 1 , 4 -dihydroqumolme-3 -carboxamide; m.p. 214-216°C (compound 90) . (kkkk) N-{4- [ [1-4- (2- Pyridmyl) piperazmyl] methyl] benzyl) 6 -ethoxy-4 -oxo- 1 , 4- dihydroqumolme-3 -carboxamide; m.p. 202 -204 °C (compound 91) . (1111) N-{4- [1- [4- (2- Pyrimidmyl) piperazmyl] methyl] benzyl } 6-ethoxy-4-oxo-l , 4- dihydroqumolme-3 -carboxamide; m.p. 198-201°C (compound 92) . (mmmm) N- {4- [1- [4- (4- Chlorophenyl) piperazmyl] methyl] benzyl} 6 -ethoxy-4 -oxo-1 , 4- dihydroqu olme- 3 -carboxamide; m.p .260-263 °C (compound 93). (nnnn) N-{4- [1- [4- (4- Fluorophenyl ) piperazmyl] methyl] benzyl } 6 -ethoxy-4 -oxo- 1 , 4 - dihydroqumolme-3 -carboxamide; m.p.218-220°C (compound 94). (oooo) N- {4- [1- (4-Acetylpιperazmyl) methyl] benzyl} 6 -ethoxy-4 -oxo-1 , 4-dιhydroqumolme-3 -carboxamide; wax (compound 95) . (pppp) N- {4- [1- (4-Benzylpιperazmyl) methyl] benzyl} 6 -ethoxy-4 -oxo-1 , 4 -dihydroqumolme- 3 -carboxamide ; m.p. 183-185°C (compound 96) . (qqqq) N- {4 -[ (N-Benzyl -N-ethylammo) methyl] benzyl } 6 -ethoxy-4 -oxo-1 , 4 -dihydroqumolme-3 -carboxamide; m.p. 129-132°C (compound 97) . (rrrr) N-{4- [ (N-Benzyl -N-methylammo) methyl] benzyl } 6-ethoxy-4-oxo-l , 4 -dihydroqumolme-3-carboxamide ; m.p. 129- 131°C (compound 98) . (ssss) N-{4- [ (N-Methyl-N- phenethylammo) methyl] benzyl } 6 -ethoxy-4 -oxo-1 , 4- dihydroqumolme-3 -carboxamide; m.p. 137-139°C (compound 99) . (tttt) N-{4- [ [1- (4-Methyl) piperazmyl] methyl] benzyl} 6 -ethoxy-4 -oxo-1,4-dihydroqumolme-3 -carboxamide ; m.p. 174-177°C (compound 100) . (uuuu) N-{4- [ [1- (4- Piperonyl) piperazmyl] methyl] benzyl} 6 -ethoxy-4 -oxo- 1 , 4- dihydroqumolme-3 -carboxamide; m.p. 193-195°C (compound 101) . (vvw) N- {4- [ (1-Pιperιdmyl) methyl] benzyl} 6-ethoxy- 4-oxo-l, 4 -dihydroqumolme- 3 -carboxamide; m.p. 172-174°C (compound 102 ) . (wwww) N- {4- [ (1-Pyrrolιd yl) methyl] benzyl } 6- ethoxy-4 -oxo-1, 4 -dihydroqumolme-3 -carboxamide; m.p. 168- 171°C (compound 103) . (xxxx) N-{4- [1- (1,2,3,4- Tetrahydroisoqumol yl) methyl] benzyl} 6 -ethoxy-4 -oxo- 1 , 4- dihydroqumolme- 3 -carboxamide; m.p. 167-169°C (compound 104) . (yyyy) N ~ ( 3 ~ [ ( 4 -Morpholmyl) methyl] benzyl} 6- methoxymethyl-4 -oxo-1 , 4 -dihydroqumolme- 3 -carboxamide ; m.p . 151-153°C (compound 105) . (zzzz) N-{3- [1- [4- (3- Tπfluoromethylphenyl ) piperazmyl] methyl] benzyl } 6-ethoxy-4- oxo-1 , 4 -dihydroqumolme-3 -carboxamide ; m.p. 216-218°C (compound 106). (aaaaa) N- { 3 -[ [1- (4 -Benzyl) piperazmyl] methyl] benzyl } 6 -ethoxy-4 -oxo-1,4-dihydroqumolme-3 -carboxamide ; m.p. 161-164 °C (compound 107) . (bbbbb) N- {3- [ (N-Benzyl -N-methylammo) methyl] benzyl} - 6 -ethoxy-4 -oxo-1 , 4 -dihydroqumolme- 3 -carboxamide; m.p. 144- 146°C (compound 108) . (ccccc) N- {3- [ [- (4 -Methyl) piperazmyl] methyl] benzyl} -6- ethoxy-4 -oxo-1, 4 -dihydroqumolme-3 -carboxamide ; m.p. 208- 210°C (compound 109) . (ddddd) N-{3-[[l-(4- Pheny) lpiperazmyl] methyl] benzyl} - 6 -ethoxy-4 -oxo- 1 , 4- dihydroqumolme- 3 -carboxamide; m.p. 221-223°C (compound 110) . (eeeee) N{ [3- [1- (Pyrrolidmyl) methyl] benzyl } -6- ethoxy-4 -oxo- 1 , 4 -dihydroqumolme-3 -carboxamide ; m.p. 184- 186 °C (compound 111) . (fffff ) N-{4- [4- (l,2,3,4-4H-Pyrazmo [1,2- a] benzimadazolyl) methyl] benzyl } -6 -ethoxy-4 -oxo-1 , 4- dihydroqu olme- 3 -carboxamide; m.p. 271-273°C (compound 112) . (gg g) N-{4- [4- (l,2,3,4,5,6-6H-l,4-Dιazepmo[l,2- a] benzimidazolyl) methyl] benzyl } -6-ethoxy-4-oxo-l , 4- dihydroqumolme- 3 -carboxamide; m.p. 252 -254 °C (compound 113) . (hhhhh) N- [3- (1-Methylammoethyl) benzyl] -6-chloro-4- oxo-1 , 4 -dihydroqumolme-3 -carboxamide hydrochloride; m.p. 184°C (dec.) (compound 114). (mil) N-[4- (4-Morpholmylmethyl) benzyl] -6-chloro-4- oxo-1 , 4 -dihydroqumolme-3 -carboxamide ; (compound 115). X) 31 U 1 ) N- {4- [1- (4-Methylpιperazmyl) methyl] benzyl } - 6 -chloro-4 -oxo- 1 , 4 -dihydroqumolme-3 -carboxamide; m.p. 162- 166°C (compound 116) . (kkkkk) N- [3- (4 -Morpholmylmethyl) benzyl] -6-ethoxy-4- oxo-1 , 4 -dιhydroqumolme-3 -carboxamide ; m.p. 173-177°C (compound 117) . (11111) N- [3- (4 -Morpholmylmethyl) benzyl] -4-oxo-l, 4- dihydroqumolme-3 -carboxamide hydrochloride; m.p. 203-208°C (compound 118) . (mmmmm) N- [4- (4 -Morpholmylmethyl) benzyl] -4-oxo-l , 4- dihydroqu olme-3 -carboxamide hydrochloride; m.p. 177-180°C (compound 119) . (nnnnn) N- {4- [1- (4-Methylpιperazmyl) methyl] benzyl} - 4-oxo-l , 4 -dihydroqumolme-3 -carboxamide hydrochloride; m.p. 262 -266 °C (compound 120) . (ooooo) N- [3- (4 -Morpholmylmethyl) benzyl] -6-chloro-4- oxo-1 , 4 -dihydroqumolme-3 -carboxamide ; (compound 121). (ppppp) N- [3- (1-Pyrrolιdmylmethyl) benzyl] -4-oxo-6- chloro-1 , 4 -dihydroqumolme-3 -carboxamide; m.p. 153-160°C (compound 122) . (qqqqq) [4- (2 , 2 , 2-Trιfluoroethylammomethyl) benzyl] - 4-oxo-l , 4 -dihydroqumolme-3-carboxamide; m.p. 140-141°C (compound 123) . (rrrrr) N- [4- (1-Pιperιdmylmethyl) benzyl] -6-methoxy- 4-oxo-l , 4 -dihydroqumolme-3 -carboxamide; m.p. 203-205°C (compound 124) . ( sssss ) N- [4 - ( 1 -Pιperιdmylmethyl ) benzyl ] - 6 - fluoro-4 - oxo- 1 , 4 -dihydroqumol me- 3 -carboxamide hydrochloride ; m. p . >115 ° C ( compound 125 ) . (ttttt) N-{4- [1- (2-Methylιmιdazolyl)methyl]benzyl}-6- fluoro-4 -oxo- 1 , 4 -dihydroqumol e-3 -carboxamide hydrochloride; m.p. 120-122°C (compound 126) . (uuuuu) N- [3- (4 -Morpholmylmethyl) benzyl] -6-methyl-4- oxo-1 , 4 -dihydroqumolme-3 -carboxamide ; m.p. 140-144°C (compound 127) . (wvw) N- [4- (1-Pyrrolιdmylmethyl) benzyl] -6-fluoro- 4-oxo-l , 4 -dihydroqumolme- 3 -carboxamide hydrochloride; m.p. 192-194°C (compound 128) . (wwwww) N- [4- (4 -Morphol ylmethyl) benzyl] -6-methoxy- 4-oxo-l , 4 -dihydroqumolme-3 -carboxamide hydrochloride; m.p. 150-152°C (compound 129) . (xxxxx) N- [3- (N-Benzyl -N-methylammomethyl) benzyl] -6- methoxy-4 -oxo-1 , 4 -dihydroqumolme-3 -carboxamide ; m.p. 186- 188°C (compound 130) . (yyyyy) N- { 3 - [l- (4 -Methylpiperazmyl ) methyl] benzyl } - 6-methoxy-4-oxo-l , 4 -dihydroqumolme-3 -carboxamide ; m.p . 179-181°C (compound 131) . (zzzzz) [4- (2 , 2 , 2-Trιfluoroethylammomethyl) benzyl] - 6-chloro-4-oxo-l , 4 -dihydroqumolme-3 -carboxamide; m.p. 171- 172 °C (compound 132) . (aaaaaa) N-{3- [4- (2- Pyπmidmyl ) piperazmylmethyl] benzyl } -6-methoxy-4-oxo-l , 4- dιhydroqumolme-3 -carboxamide ; m.p. 193-195°C (compound 133) . (bbbbbb) N-{3-[l-(l,2,3,4- Tetrahydroisoqumolmyl) methyl] benzyl } -6-ethoxy-4-oxo-l , 4- dihydroqumolme-3 -carboxamide ; m.p. 145-147°C (compound 134) . (ccccc) N- [3 - (4 -Morpholmylmethyl) benzyl] -6-fluoro-4- oxo-1 , 4 -dihydroqumolme- 3 -carboxamide ; (compound 135). (dddddd) N- {4- [2- (Ethoxyethoxy) methyl] benzyl} -4-oxo- 1 , 4 -dihydroqumolme-3-carboxamide; wax (compound 136). (eeeeee) N- {4- [2- (Cyclohexoxyethoxy) methyl] benzyl } -6- ethoxy-4 -oxo- 1 , 4 -dihydroqumolme-3 -carboxamide ; wax (compound 137) . (ffffff) N-{4- [2- (Phenoxyethoxy) methyl] benzyl} -4 -oxo- 1, 4 -dihydroqumolme-3 -carboxamide; wax (compound 138). (gggggg) N- (R) - [3- ( 1 -Methylaminoethyl ) benzyl] -6- chloro-4 -oxo-1, 4 -dihydroqumolme-3 -carboxamide; (compound 139 ) . (gggggg) N- ( S ) - [3 - ( 1 -Methylaminoethyl ) benzyl ] - 6 - chloro - 4 - oxo - 1 , 4 - dihydroqumol me - 3 - carboxamide ; ( compound 140 ) . Example 5 Preparation of radiolabeled probe compounds of the invention The compounds of the invention are prepared as radiolabeled probes by carrying out their synthesis using precursors comprising at least one atom that is a radioisotope. The radioisotope is preferably selected from of at least one of carbon (preferably 1 C) , hydrogen (preferably 3 H) , sulfur (preferably 35 S) , or iodine (preferably 125 I) . Such radiolabeled probes are conveniently synthesized by a radioisotope supplier specializing in custom synthesis of radiolabeled probe compounds. Such suppliers include Amersham Corporation, Arlington Heights, IL; Cambridge Isotope Laboratories, Inc. Andover, MA; SRI International, Menlo Park, CA; Wizard Laboratories, West Sacramento, CA; ChemSyn Laboratories, Lexena, KS ; American Radiolabeled Chemicals, Inc., St. Louis, MO; and Moravek Biochemicals Inc., Brea, CA. Tritium labeled probe compounds are also conveniently prepared catalytically via platinum-catalyzed exchange in tritiated acetic acid, acid-catalyzed exchange in tritiated tπfluoroacetic acid, or heterogeneous-catalyzed exchange with tritium gas. Such preparations are also conveniently carried out as a custom radiolabelmg by any of the suppliers listed m the preceding paragraph using the compound of the invention as substrate. In addition, certain precursors may be subjected to tritium-halogen exchange with tritium gas, tritium gas reduction of unsaturated bonds, or reduction using sodium borotπtide, as appropriate . Example 6 Receptor autoradiography Receptor autoradiography (receptor mapping) is carried out vitro as described by Kuhar in sections 8.1.1 to 8.1.9 of Current Protocols in Pharmacology (1998) John Wiley & Sons, New York, using radiolabeled compounds of the invention prepared as described the preceding Example. Example 7 Binding Assay The following assay is a standard assay of GABA A receptor binding. The high affinity and high selectivity of compounds of this invention for the benzodiazepine site of the GABA A receptor is shown using the binding assay described in Thomas and Tallman (J. Bio . Chem . 1981; 156:9838-9842, and J. Neurosci . 1983; 3:433-440). Rat cortical tissue is dissected and homogenized m 25 volumes (w/v) of Buffer A (0.05 M Tris HCl buffer, pH 7.4 at 4 °C) . The tissue homogenate is centπfuged m the cold (4 °C) at 20,000 x g for 20 minutes. The supernatant is decanted, the pellet rehomogenized m the same volume of buffer, and centπfuged again at 20,000 x g. The supernatant of this centrifugation step is decanted and the pellet stored at -20 °C overnight. The pellet is then thawed and resuspended m 25 volumes of Buffer A (original wt/vol) , centrifuged at 20,000 x g and the supernatant decanted. This wash step is repeated once. The pellet is finally resuspended in 50 volumes of Buffer A. Incubations containi 100 μl of tissue homogenate, 100 μl of radioligand, (0.5 nM 3 H-Rol5-1788 [ 3 H-Flumazenil] , specific activity 80 Ci/mmol), and test compound or control (see below) , and are brought to a total volume of 500 μl with Buffer A. Incubations are carried for 30 min at 4°C and then rapidly filtered through Whatman GFB filters to separate free and bound ligand. Filters are washed twice with fresh Buffer A and counted in a liquid scintillation counter. Nonspecific binding (control) is determined by displacement of 3 H Rol5-1788 with 10 μM Diazepam (Research Biochemicals International, Natick, MA). Data were collected in triplicate, averaged, and percent inhibition of total specific binding (Total Specific Binding = Total - Nonspecific) was calculated for each compound. A competition binding curve is obtained with up to 11 points spanning the compound concentration range from 10 "12 M to 10 "5 M obtained per curve by the method described above for determining percent inhibition. K λ values are calculated according the Cheng-Prussof equation. When tested in this assay compounds of the invention exihibit K values of less than 1 uM, preferred compounds of the invention have K ^ values of less than 500 nM and more preferred compounds of the invention have Rvalues of less than 100 nM. Example 8 Electrophysiology The following assay is used to determine if a compound of the invention act as an agonist, an antagonist, or an inverse agonist at the benzodiazepine site of the GABA A receptor. Assays are carried out as described in White and Gurley (NeuroReport 6: 1313-1316, 1995) and White, Gurley, Hartnett, Stirling, and Gregory (Receptors and Channels 3.: 1-5, 1995) with modifications. Electrophysiological recordings are carried out using the two electrode voltage- clamp technique at a membrane holding potential of -70 mV. Xenopus Laevi s oocytes are enzymatically isolated and injected with non-polyadenylated cRNA mixed m a ratio of 4:1:4 for α, β and γ subunits, respectively. Of the nine combinations of α, β and γ subunits described m the White et al . publications, preferred combinations are ^ 2 Y 2 , a 2 β 3 γ 2 a 3 β 3 γ ^ and α 5 β 3 γ 2 Preferably all of the subunit cRNAs in each combination are human clones or all are rat clones. The sequence of each of these cloned subunits is available from GENBANK, e.g., human α 1# GENBANK accession no. X14766, human α 2 , GENBANK accession no. A28100; human α 3 , GENBANK accession no. A28102; human α 3 , GENBANK accession no. A28104; human β 2 , GENBANK accession no. M82919; human β 3 , GENBANK accession no. Z20136; human β 2 , GENBANK accession no. X15376; rat α x , GENBANK accession no. L08490, rat α 2 , GENBANK accession no. L08491; rat α 3 , GENBANK accession no. L08492; rat α 5 , GENBANK accession no. L08494; rat β 2 , GENBANK accession no. X15467; rat β 3 , GENBANK accession no. X15468; and rat γ 2 , GENBANK accession no. L08497. For each subunit combination, sufficient message for each constituent subunit is injected to provide current amplitudes of >10 nA when 1 μM GABA is applied. Compounds are evaluated against a GABA concentration that evokes <10% of the maximal evokable GABA current (e.g. 1 μM - 9 μM) . Each oocyte is exposed to increasing concentrations of compound in order to evaluate a concentration/effect relationship. Compound efficacy is calculated as a percent-change in current amplitude: 100* ( (Ic/I) -1) , where Ic is the GABA evoked current amplitude observed m the presence of test compound and I is the GABA evoked current amplitude observed the absence of the test compound. Specificity of a compound for the benzodiazepine site is determined following completion of a concentration/effect curve. After washing the oocyte sufficiently to remove previously applied compound, the oocyte is exposed to GABA + 1 μM R015-1788, followed by exposure to GABA + 1 μM R015- 1788 + test compound. Percent change due to addition of compound is calculated as described above. Any percent change observed m the presence of R015-1788 is subtracted from the percent changes m current amplitude observed in the absence of 1 μM R015-1788. These net values are used for the calculation of average efficacy and EC 50 values by standard methods. To evaluate average efficacy and EC S0 values, the concentration/effect data are averaged across cells and fit to the logistic equation. The invention and the manner and process of making and using it, are now described m such full, clear, concise and exact terms as to enable any person skilled m the art to which it pertains, to make and use the same. It is to be understood that the foregoing describes preferred embodiments of the present invention and that modifications may be made therein without departing from the spirit or scope of the present invention as set forth in the claims. To particularly point out and distinctly claim the subject matter regarded as invention, the following claims conclude this specification.

Claims: What is claimed is: 1. A compound of the formula or a pharmaceutically acceptable salt thereof wherein: R x and R 2 are the same or different and represent hydrogen, halogen, lower alkyl, lower alkoxy, lower alkoxyalkyl, or cycloalkyl or cycloalkyl alkoxy, where each cycloalkyl group has from 3-7 members, where up to two of the cycloalkyl members are optionally hetero atoms selected from oxygen and nitrogen, and where any member of the cycloalkyl group is optionally substituted with halogen, lower alkyl or lower alkoxy; and W is hydrogen; or W is cycloalkyl having from 3-7 members, where up to two of the members are optionally hetero atoms selected from oxygen and nitrogen, and where any member of the cycloalkyl group is optionally substituted with halogen, lower alkyl or lower alkoxy; or W is lower alkyl optionally substituted with up to three groups selected from: 1) hydroxy, lower alkoxy, halogen, li ) cycloalkyl having from 3-7 members, where up to two of the members are optionally hetero atoms selected from oxygen and nitrogen, and where any member of the cycloalkyl group is optionally substituted with halogen, lower alkyl or lower alkoxy, and m) aryl or heteroaryl optionally mono-, di-, or tri- substituted with: a) halogen, hydroxy, lower alkyl, lower alkoxy, arylalkyl, heteroarylalkyl, b) -NR 5 R, wherein R 5 and R 6 are the same or different and represent hydrogen, lower alkyl, or arylalkyl , c) - (CH 2 ) n O (CH 2 ) m R 7 wherein n and m are independently 0, 1, 2 or 3 and R- is lower alkoxy, aryl, heteroaryl, amino, mono- or dialkylamino, or cycloalkyl or cycloalkylalkoxy, where each cycloalkyl group has from 3-7 members, and where up to two of the cycloalkyl members are optionally hetero atoms selected from oxygen and nitrogen, or d) :CH 2 ),.NR 8 R 9 wherein y is 0, 1, 2 or 3 and R 8 and R Q are the same or different and represent hydrogen, lower alkyl optionally substituted with trifluormethyl , aryl, heteroaryl, arylalkyl, heteroarylalkyl, or cycloalkylalkyl , where the cycloalkyl portion has from 3-7 members, and where up to two of the members are optionally hetero atoms selected from oxygen and nitrogen, or NR 8 R 9 together represent piperidinyl, 1,2,3,4- tetrahydroisoquinolinyl , morpholinyl, 4- oxopiperidinyl , or wherein R 10 is hydrogen, lower alkyl, acetyl or aryl , heteroaryl , arylalkyl, diarylalkyl or heteroarylalkyl, where each aryl portion is optionally substituted with up to three groups selected from halogen, hydroxy, lower alkyl, lower alkoxy, acetyl, or aryl. 2. A compound of the formula or the pharmaceutically acceptable salts thereof wherein: R- L is hydrogen, halogen, lower alkyl, lower alkoxy, lower alkoxyalkyl, or cycloalkyl or cycloalkyl alkoxy, where each cycloalkyl group has from 3-7 members, where up to two of which members are optionally hetero atoms selected from oxygen and nitrogen, and where any member of the cycloalkyl group is optionally substituted with halogen, lower alkyl or lower alkoxy; and R n IS l) hydroxy, lower alkoxy, halogen, li ) lower alkyl optionally mono- or disubstituted with lower alkoxy, in) cycloalkyl having from 3-7 members, shere up to two of which members are optionally hetero atoms selected from oxygen and nitrogen, and where any member of the cycloalkyl group is optionally substituted with halogen, lower alkyl or lower alkoxy, or IV) aryl or heteroaryl optionally mono-, di-, or tri- substituted with a) halogen, hydroxy, lower alkyl, lower alkoxy, heteroarylalkyl , b) -NR 5 R 6 wherein R 5 and R 6 are the same or different and represent hydrogen, lower alkyl, or arylalkyl, c) - (CH 2 ) n O (CH 2 ) m R 7 wherein n and m are independently 0, 1, 2 or 3 and R 7 is lower alkoxy, aryl, heteroaryl, ammo, mono- or dialkylam o, or cycloalkyl or cycloalkylalkoxy, where each cycloalkyl group has from 3-7 members, and where up to two of the members are optionally hetero atoms selected from oxygen and nitrogen, or d) - (CH- y NRgRg wherein y is 0, 1, 2 or 3 and R 8 and R 9 are the same or different and represent hydrogen, lower alkyl optionally substituted with trifluormethyl , aryl, heteroaryl, arylalkyl, heteroarylalkyl, or cycloalkylalkyl , where the cycloalkyl portion has from 3-7 members, and where up to two of the members are optionally hetero atoms selected from oxygen and nitrogen, or NR 8 Rg together represent piperidinyl , 1,2,3,4- tetrahydroisoquinolmyl , morpholinyl, 4- oxopiperidinyl , or wherein R 10 is hydrogen, lower alkyl, acetyl or aryl, heteroaryl, arylalkyl, diarylalkyl or heteroarylalkyl, where each aryl portion is optionally substituted with up to three groups selected from halogen, hydroxy, lower alkyl, lower alkoxy, acetyl, or aryl. A compound of the formula or the pharmaceutically acceptable salts thereof wherein: R λ is hydrogen, halogen, lower alkyl, lower alkoxy, lower alkoxyalkyl, or cycloalkyl or cycloalkyl alkoxy, where each cycloalkyl group has from 3-7 members, where up to two of the members are optionally hetero atoms selected from oxygen and nitrogen, and where any member of the cycloalkyl group is optionally substituted with halogen, lower alkyl or lower alkoxy; n is 1, 2, 3 or 4 ; and X is CH 2 , 0 or NR 12 , where R 12 is hydrogen or lower alkyl. A compound according to claim 1 of the formula or the pharmaceutically acceptable salts thereof wherein: R :5 is hydrogen or C J^ -C J lower alkyl; R : is hydrogen, halogen, lower alkyl, lower alkoxy, lower alkoxyalkyl , or cycloalkyl or cycloalkyl alkoxy, where each cycloalkyl group has from 3-7 members, where up to two of the members are optionally hetero atoms selected from oxygen and nitrogen, and where any member of the cycloalkyl group is optionally substituted with halogen, lower alkyl or lower alkoxy; and R :2 , R 12 ., R- 13 / R 14 / and R 14 , are the same or different and represent a) hydrogen, halogen, hydroxy, lower alkyl, lower alkoxy, heteroarylalkyl, b) -NR 5 R 6 wherein R 5 and R 6 are the same or different and represent hydrogen, lower alkyl, or arylalkyl, c) - (CH 2 ) n O (CH 2 ) m R 7 wherein n and m are independently 0, 1 , 2 or 3 and R 7 is lower alkoxy, aryl, heteroaryl, amino, mono- or dialkylamino, or cycloalkyl or eyeloalkylalkoxy, where each cycloalkyl group has from 3-7 members, and where up to two of the members are optionally hetero atoms selected from oxygen and nitrogen, or wherein y is 0, 1, 2 or 3 and R 8 and R 9 are the same or different and represent hydrogen, lower alkyl optionally substituted with trifluormethyl , aryl, heteroaryl, arylalkyl, heteroarylalkyl, or cycloalkylalkyl , where the cycloalkyl portion has from 3-7 members, and where up to two of the members are optionally hetero atoms selected from oxygen and nitrogen, or NR 8 R 9 together represent piperidinyl, 1,2,3,4- tetrahydroisoquinolinyl , morpholinyl, 4- oxopiperidinyl , or wherein R 10 is hydrogen, lower alkyl, acetyl or aryl, heteroaryl, arylalkyl, diarylalkyl or heteroarylalkyl, where each aryl portion is optionally substituted with up to three groups selected from halogen, hydroxy, lower alkyl, lower alkoxy, acetyl, or aryl, provided that at least two of R 12 R 12 ., R 13 , R 14 , and R 14 , are hydrogen . 5. A compound according to claim 1 of the formula or the pharmaceutically acceptable salts thereof wherein: is defined the same as m Claim 1 ; R 14 is hydrogen, halogen, lower alkyl or lower alkoxy; B is CH, NH, S or 0; and C is S, CH 2 , CH, or N. 6. A compound according to claim 1 of the formula or the pharmaceutically acceptable salts thereof wherein: R x is defined the same as in Claim 1; R 14 is hydrogen, halogen, lower alkyl or lower alkoxy; and A, B and C are independently CH or N, provided that no more than two of A, B, or C are N. 7. A compound according to claim 1, which is selected from: N- [2- (2 -Hydroxyethoxy) ethyl] 4-oxo-l , 4 -dihydroqumolme-3 -carboxamide ; N- 1soamy1 -4 -oxo-1,4 -dihydro-quinolme-3 -carboxamide ; N-Benzyl -4 -oxo-1, 4 -dihydro-qumolme-3 -carboxamide ; N- (2-Fluorobenzyl) -4-oxo-l, 4 -dihydro-qumolme-3- carboxamide ; N- (3-Fluorobenzyl) -4-oxo-l , 4 -dihydro-qumol e-3 - carboxamide ; N- (4-Fluorobenzyl) -4-oxo-l , 4 -dihydro-qumol e-3 - carboxamide ; N- [ (2-Furanyl) methyl] -4-oxo-l , 4-dihydro-qumolme-3 - carboxamide ; N- (4-Methoxybenzyl) -4-oxo-l , 4 -dihydro-qumolme- 3 - carboxamide; and N-Piperonyl -4 -oxo-1,4 -dihydro-qumolme-3 -carboxamide ; N- (3 , 4-Dιmethoxybenzyl) -4-oxo-l , 4 -dihydro-qumolme-3 - carboxamide . 8. A compound according to claim 1, which is N-[(2- Thienyl) methyl] -4-oxo-l, 4 -dihydro-qumolme-3 -carboxamide . 9. A compound according to claim 1, which is N- [6- (2 , 3 -Dihydro-1,4 -benzodioxmyl) methyl] -4-oxo-l, 4 -dihydroqumolme- 3 -carboxamide . 10. A compound according to claim 1, which is selected from N- (2-Fluoro-4-ethoxybenzyl) -4-oxo-l , 4-dιhydro- qumolme-3 -carboxamide ; N- (4-Ethoxybenzyl) -4-oxo-l , 4-dιhydro-qumolιne-3 - carboxamide; N- (3-Ethoxypropyl) -4-oxo-l , 4 -dihydro-qumoline-3 - carboxamide ; N- (2-Fluoro-4-ιsopropoxybenzyl) -4-oxo-l , 4-dιhydro- qumolme-3 -carboxamide ; N- [4- (Methylammomethyl) benzyl] -4-oxo-l, 4 -dihydroqumolme-3 -carboxamide hydrochloride; and N- [3- (1-Imιdazolyl) propyl] -4 -oxo-1 , 4 -dihydro-qumolme- 3 -carboxamidehydrochloπde . 11. A compound according to claim 1, which is selected from: N- [4- (1 -Methylaminoethyl) benzyl] -4-oxo-l , 4 -dihydroqumolme-3 -carboxamide hydrochloride ; N- [4- (Ethylammomethyl) benzyl] -4 -oxo-1 , 4 -dihydroqumolme-3 -carboxamide hydrochloride; N- [4- (1-Imidazolylmethyl) benzyl] -4 -oxo-1 , 4 -dihydroqumolme- 3 -carboxamide ; N- [4- (Methylammomethyl) benzyl] -6-chloro-4 -oxo-1 , 4- dιhydro-qumolme-3 -carboxamide hydrochloride; N- [4- (Ethylammomethyl) benzyl] -6-chloro-4-oxo-l , 4- dιhydro-qumolme-3 -carboxamide ; N- [4- (Dimethylammomethyl) benzyl] -6-chloro-4-oxo-l , 4- dιhydro-qumolme-3 -carboxamide hydrochloride ; N- [3- (Dimethylammomethyl) benzyl] -6-chloro-4-oxo-l , 4- dιhydro-qumolme-3 -carboxamide hydrochloride; and N- [3- (Ethylammomethyl) benzyl] -6-chloro-4-oxo-l , 4- dihydro-qumolme-3 -carboxamide . 12. A compound according to claim 1, which is selected from: N- [4- (1-Imidazolylmethyl) benzyl] -6-chloro-4-oxo-l , 4-dιhydro- qumolme-3 -carboxamide; and N- [3- (1-Imidazolylmethyl) benzyl] -6-chloro-4-oxo-l , 4-dιhydro- qumolme-3 -carboxamide . 13. A compound according to claim 1, which is selected from: N-Benzyl -6 -methoxymethyl -4 -oxo-1, 4 -dihydro-qumolme- 3 - carboxamide ; N- [ (2 -Thienyl ) methyl ] - 6 -methoxymethyl -4 -oxo- 1 , 4 - dιhydro-qumolme-3 - carboxamide ; N- [ (3 -Ethoxy) propyl] -6 -methoxymethyl -4 -oxo-1 , 4 -dihydroqumolme-3 -carboxamide ; N-Butyl- 6 -methoxymethyl -4 -oxo-1 , 4 -dihydro-qumolme- 3 - carboxamide ; N- [4- (Methylammomethyl) benzyl] -6 -methoxymethyl -4 -oxo- 1 , 4 -dihydro-qumolme-3 -carboxamide ; and N- [3- (Methylammomethyl) benzyl] -6 -methoxymethyl -4 -oxo- 1 , 4 -dιhydro-qumolme-3 -carboxamide . 14. A compound according to claim 1, which is N-Butyl- 6- (1-morpholmo) -4-oxo-l , 4-dιhydro-qumolme-3-carboxamιde . 15. A compound according to claim 1, which is N-[(2- Thienyl ) methyl] -6- (1-morpholmo) -4-oxo-l , 4 -dihydroqumolme-3 -carboxamide . 16. A compound according to claim 1, which is selected from: N-Benzyl -6 -ethoxy-4 -oxo-1 , 4 -dihydro-qumolme-3 - carboxamide; and N- (4-Ethoxybenzyl) -6 -ethoxy-4 -oxo-1 , 4-dιhydro- qumol e-3 -carboxamide . 17. A compound according to claim 1, which is N-[(2- Thienyl) methyl] -6 -ethoxy-4 -oxo-1 , 4 -dihydro-qumolme-3 - carboxamide . 18. A compound according to claim 1, which is selected from: N- (3-Ethoxypropyl) -6-ethoxy-4 -oxo-1 , 4 -dihydroqumolme-3 -carboxamide ; N- [3- (Isopropoxy) propyl] -6-ethoxy-4-oxo-l , 4 -dihydro- quιnolme-3 -carboxamide ; N- [ (2 -Tetrahydrofuranyl) methyl] -6-ethoxy-4-oxo-l , 4- dihydro-qumolme-3 -carboxamide ; N-Isoamyl-6-ethoxy-4-oxo-l, 4 -dihydro-qumolme-3 - carboxamide ; N-Cyclohexyl-6-ethoxy-4-oxo-l , 4 -dihydro-qumolme-3 - carboxamide ; N-Pιperonyl-6-ethoxy-4-oxo-l , 4 -dihydro-qumolme-3 - carboxamide; and N- [3- (Methoxy) propyl] -6-ethoxy-4-oxo-l, 4-dιhydro- qumolme- 3 -carboxamide . 19. A compound according to claim 1, which is selected from: N- (2-Fluorobenzyl) -6-ethoxy-4 -oxo-1 , 4-dιhydro- qumolme- 3 -carboxamide ; and N- (3 -Fluorobenzyl) -6-ethoxy-4-oxo-l , 4 -dihydroqumolme-3 -carboxamide . 20. A compound according to claim 1, which is selected from-. N- [ (2 -Pyπdyl) methyl] -6-ethoxy-4-oxo-l, 4-dιhydro- qu olme-3 -carboxamide ; and N- [ (3-Pyπdyl) methyl] -6-ethoxy-4-oxo-l, 4 -dihydroqumolme-3 -carboxamide . 21. A compound according to claim 1, which is selected from: N-Butyl-6 -ethoxy-4 -oxo-1 , 4 -dihydro-qumolme-3- carboxamide ; N-Pentyl-6-ethoxy-4-oxo-l , 4 -dihydro-qumolme-3 - carboxamide; N-Propyl- 6 -ethoxy-4 -oxo-1 , 4 -dihydro-qumolme-3 - carboxamide ; N- [ (2 -Metnyl ) propyl] -6-ethoxy-4-oxo-l , 4 -dihydroqumolme-3 -carboxamide; N-Hexyl -6-ethoxy-4 -oxo- 1 , 4 -dihydro-qumolme-3 - carboxamide; and N-Methyl-6-ethoxy-4-oxo-l , 4 -dihydro-qumolme-3 - carboxamide . 22. A compound according to claim 1, which is N-[(3- Thienyl) methyl] -6 -ethoxy-4 -oxo-1 , 4 -dihydro-qumolme-3 - carboxamide . 23. A compound according to claim 1, which is selected from: N- [4- (Methylammomethyl) benzyl] -6 -ethoxy-4 -oxo-1 , 4- dihydro-qumolme-3 -carboxamide hydrochloride ; N- [4- (Ethylammomethyl) benzyl] -6-ethoxy-4-oxo-l , 4- dihydro-qumolme-3-carboxamide hydrochloride; N- [4- (1 -Methylaminoethyl) benzyl] -6-ethoxy-4-oxo-l , 4- dihydro-qu olme- 3 -carboxamide hydrochloride; N- [3 - (Methylammomethyl) benzyl] -6-ethoxy-4-oxo-l , 4- dιhydro-qumolme-3 -carboxamide hydrochloride; and N- {3- [ (1 -Methyl) ammoethyl] benzyl] - 6 -ethoxy-4 -oxo- 1 , 4- dιhydro-qumolme-3 -carboxamide hydrochloride . 24. A compound according to claim 1, which is selected from: N- [3- [ (1-Imidazolyl) methyl] benzyl} -6 -ethoxy-4 -oxo-1 , 4- dihydro-qumolme- 3 -carboxamide; and N- {4 - [ (1-Imidazolyl) methyl] benzyl } -6 -ethoxy-4 -oxo-1 , 4 - dihydro-qumolme-3 -carboxamide . 25. A compound according to claim 1, which is selected from: N-Benzyl-6 -methoxy-4 -oxo-1 , 4 -dihydro-qumolme-3 - carboxamide ; N- [4- (Methylammomethyl) benzyl] -6-methoxy-4-oxo-l , 4- dιhydro-qumolme-3 -carboxamide hydrochloride; N- [4- (Dimethylammomethyl) benzyl] -6 -ethoxy-4 -oxo-1 , 4- dihydro-qumolme-3 -carboxamide hydrochloride; N- [4- (Methylammomethyl) benzyl] -7-chloro-4-oxo-l , 4- dihydro-qumolme-3 -carboxamide hydrochloride ; N-Benzyl -8 -fluoro-4 -oxo-1 , 4 -dihydro-qumolme-3 - carboxamide hydrochloride; N-Butyl -8 -fluoro-4 -oxo-1 , 4 -dihydro-qumolme-3 - carboxamide hydrochloride; N-4 -Fluorobenzyl-6-chloro-4-oxo-l , 4 -dihydroqumolme- 3- carboxamide ; and N- { [4- [2- (1-Cyclohexyl) ethoxy] methyl] benzyl} -6-ethoxy- 4 -oxo-1 , 4 -dihydroqumolme-3 -carboxamide . 26. A compound according to claim 1, which is N-{4-[l- (2-Pyrιdyl) methoxy] methyl] benzyl } -6-ethoxy-4-oxo-l , 4- dihydroqumolme-3 -carboxamide . 27. A compound according to claim 1, which is selected from: N-{4- [1- [4- (2- Quinolmylmethyl) piperazmyl] methyl] }benzyl} -6-ethoxy-4-oxo- 1,4 -dιhydroqumolme-3 -carboxamide ; N-{4- [1- [4- (4- Chlorobenzhydryl) piperazmyl] methyl] benzyl) -6 -ethoxy-4 -oxo- 1 , 4 -dihydroqumolme-3 -carboxamide ; and N-{4- [1- [4- (4- Acetylphenyl) piperazmyl] methyl] benzyl } -6 -ethoxy-4 -oxo-1 , 4- dιhydroqumolιne-3 -carboxamide . 28. A compound according to claim 1, which is selected from: N- { [4- [1- (4 -Pyridyl) methoxy] methyl] benzyl} -6-ethoxy-4- oxo-1,4-dihydroqumolme-3 -carboxamide ; N{4- [1- [4- (4-Bιphenylmethyl) piperazmyl] methyl] benzyl } - 6 -ethoxy-4 -oxo-1,4-dihydroqumolme-3 -carboxamide ; N- {4- [1- (4 -Oxopiperidmyl) methyl] benzyl} - 6 -ethoxy- 4-oxo-l , 4 -dιhydroqumolme-3 -carboxamide; N- [4- (Dibenzylammomethyl) benzyl] -6-ethoxy-4-oxo- 1,4-dihydro-qumolme-3 -carboxamide ; N- {4- [ [2- (4-Morpholmyl) ethyl] ammomethyl] benzyl} -6- ethoxy-4-oxo-l , 4 -dihydro-qumolme-3 -carboxamide ; N- {4- [ [3- (4-Morpholmyl) propyl] ammomethyl] benzyl } -6- ethoxy-4 -oxo-1 , 4 -dihydro-qumolme-3 -carboxamide; and N- {4- [ [2- (Diisopropylammo) ethoxy] methyl] benzyl} -6- ethoxy-4 -oxo-1 , 4 -dihydro-qumolme-3 -carboxamide . 29. A compound according to claim 1, which is selected from: N- {4- [ [2- (4-Morpholmyl) ethoxy] methyl] benzyl} -6-ethoxy- 4 -oxo-1 , 4-dιhydro-qumolme-3 -carboxamide ; N-{4- [ [ (2- (1-Piperldmyl ) ethoxy] methyl ] benzyl } - 6 - ethoxy-4 -oxo-1,4-dihydro-qumolme-3 -carboxamide ; N- {4- [ [2- (1 -Pyrrolldmyl) ethoxy] methyl] benzyl } -6- ethoxy-4 -oxo-1 , 4 -dιhydro-qumolme-3 -carboxamide; and and N- {4- [ (4-Morpholmy) methyl] benzyl } -6-ethoxy-4-oxo- 1 , 4 -dihydro-qumolme- 3 -carboxamide . 30. A compound according to claim 1, which is selected from: N-{ (4- [ [1- [4- ( 2 -Methoxyphenyl) piperazmyl] methyl] benzyl ] -6 -ethoxy-4 -oxo-1 , 4 -dihydroqumolme-3 -carboxamide ; N-{4- [ [1-4- (2-Pyrιdmyl) piperazmyl] methyl] benzyl) -6- ethoxy-4 -oxo-1 , 4 -dihydroqumolme-3 -carboxamide ; and N-{4- [1- [4- (2 -Pyrimidmyl) piperazmyl] methyl] benzyl } - 6 -ethoxy-4 -oxo- 1 , 4 -dihydroqumolme-3 -carboxamide . 31. A compound according to claim 1, which is selected from N-{4- [1- [4- (4 -Chlorophenyl) piperazmyl] methyl] benzyl} - 6 -ethoxy-4 -oxo-1 , 4 -dihydroqumolme-3 -carboxamide ; and N-{4- [1- [4- (4 -Fluorophenyl) piperazmyl] methyl] benzyl } - 6 -ethoxy-4 -oxo- 1 , 4 -dihydroqumolme-3 -carboxamide . 32. A compound according to claim 1, which is selected from: N- {4- [1- (4-Acetylpιperazmyl) methyl] benzyl} -6 -ethoxy-4 - oxo-1 , 4 -dihydroqumolme- 3 -carboxamide ; N-{4- [1- (4 -Benzylpiperazmyl) methyl] benzyl} -6-ethoxy-4- oxo-1 , 4 -dihydroqumolme-3 -carboxamide; N- {4- [ (N-Benzyl -N-ethylammo) methyl] benzyl} - 6-ethoxy-4 - oxo-1, 4 -dihydroqumolme-3 -carboxamide; N-{4- [ (N-Benzyl -N-methylammo) methyl] benzyl} - 6 -ethoxy- 4-oxo-l , 4 -dihydroqumolme-3-carboxamide ; and N- {4- [ (N-Methyl-N-phenethylammo) methyl] benzyl} -6- ethoxy-4 -oxo- 1 , 4 -dihydroqumolme-3 -carboxamide . 33. A compound according to claim 1, which is selected from: N- {4- [ [1- (4 -Methyl) piperazmyl] methyl] benzyl } -6 -ethoxy- 4 -oxo-1,4-dihydroqumolme-3 -carboxamide ; N- { 4 - [ [1 - (4 -Piperonyl ) piperazmyl] methyl] benzyl } - 6 - ethoxy-4 -oxo-1 , 4 -dιhydroqumolme-3-carboxamide ; N- {4- [ (1 -Piperldmyl) methyl] benzyl} -6-ethoxy-4 -oxo- 1 , 4- dihydroqumolme-3 -carboxamide ; and N- {4- [ (1 -Pyrrolldmyl) methyl] benzyl} -6 -ethoxy-4 - oxo-1, 4 -dihydroqumolme-3 -carboxamide . 34. A compound according to claim 1, which is N-{4-[l- (1,2,3, 4-Tetrahydroιsoqumolmyl) methyl] benzyl } - 6 -ethoxy-4- oxo-1 , 4 -dihydroqumol e-3 -carboxamide . 35. A compound according to claim 1, which is N-{3- [ (4-Morpholmyl) methyl] benzyl} -6-methoxymethyl -4 -oxo-1 , 4- dihydroqumolme-3 -carboxamide . 36. A compound according to claim 1, which is selected from: N-{3- [1- [4- (3- Trifluoromethylphenyl) piperazmyl] methyl] benzyl } -6 -ethoxy-4 - oxo-1 , 4 -dihydroqumol e-3 -carboxamide; N- {3- [ [1- (4 -Benzyl) piperazmyl] methyl] benzyl } - 6-ethoxy- 4-oxo-l , 4 -dihydroqumolme-3 -carboxamide ; and N- {3- [ (N-Benzyl -N-methylammo) methyl] benzyl } -6-ethoxy- 4-oxo-l , 4 -dihydroqumolme-3 -carboxamide . 37. A compound according to claim 1, which is selected from: N- {3- [ [- (4 -Methyl) piperazmyl] methyl] benzyl} -6 -ethoxy- 4-oxo-l , 4 -dihydroqumolme-3 -carboxamide ; N- {3- [ [1- ( -Phenyl) piperazmyl] methyl] benzyl } -6-ethoxy- 4 -oxo-1 , 4 -dihydroqumolme-3 -carboxamide ; and N{ [3- [1- (Pyrrolldmyl) methyl] benzyl} -6 -ethoxy-4 -oxo- 1 , 4 -dihydroqumolme- 3 -carboxamide . 38. A compound according to claim 1, which is selected from: N-{4-[4-(l,2,3, 4-4H-Pyrazmo [1,2- a] benzimadazolyl) ethyl] benzyl} 6 -ethoxy-4 -oxo-1 , 4- dihydroqumolme-3 -carboxamide ; and N-{4- [4- (1,2,3,4,5, 6-6H-1 , 4 -Diazepino [1,2- a] benzimidazolyl) methyl] benzyl} -6 -ethoxy-4 -oxo-1 , 4- dihydroqumolme- 3 -carboxamide . 39. A compound according to claim 1, which is selected from: N- [3- (1 -Methylaminoethyl) benzyl] -6-chloro-4-oxo-l , 4- dihydroqumolme-3 -carboxamide ; N- [4- (4 -Morpholmylmethyl) benzyl] -6-chloro-4 -oxo-1 , 4- dihydroqumolme- 3 -carboxamide ; N-{4- [1- (4 -Methylpiperazmyl) methyl] benzyl } -6-chloro- 4 -oxo- 1 , 4 -dιhydroqumolme-3 -carboxamide ; N- [3- (4 -Morpholmylmethyl) benzyl] -6-ethoxy-4-oxo-l , 4- dihydroqumolme- 3 -carboxamide ; N- [3- (4 -Morpholmylmethyl) benzyl] -4-oxo-l, 4- dihydroqumolme- 3 -carboxamide ; N- [4- (4 -Morpholmylmethyl) benzyl] -4 -oxo-1 , 4- dihydroqumolme- 3 -carboxamide ; N-{4- [1- (4 -Methylpiperazmyl) methyl] benzyl } -4-oxo-l , 4- dihydroqumolme- 3 -carboxamide ; N- [3- (4 -Morpholmylmethyl) benzyl] -6-chloro-4 -oxo-1 , 4- dihydroqumolme- 3 -carboxamide ; and N- [3- (1 -Pyrrolidmylmethyl) benzyl] -4-oxo-6-chloro-l , 4- dihydroqumolme- 3 -carboxamide . 40. A compound according to claim 1, which is selected from: [4- (2,2, 2-Trιfluoroethylammomethyl) benzyl] -4-oxo-l, 4- dιhydroqumol e-3 -carboxamide; N- [4- (1-Pιperιdmylmethyl) benzyl] -6-methoxy-4-oxo-l , 4- dihydroqumolme- 3 - carboxamide ; N- [4- (1-P perιdmylmethyl) benzyl] -6-fluoro-4 -oxo-1 , 4- dihydroqumolme-3 -carboxamide ; N- {4- [1- (2 -Methyllmidazolyl) methyl] benzyl } -6-fluoro-4- oxo-1 , 4 -dihydroqumolme-3 -carboxamide ; N- [3- (4 -Morpholmylmethyl) benzyl] -6-methyl-4 -oxo-1 , 4- dihydroqumolme- 3 -carboxamide ; N- [4- ( 1 -Pyrrolidmylmethyl) benzyl] -6-fluoro-4-oxo-l , 4- dihydroqumolme-3 -carboxamide ; and N- [4- (4 -Morpholmylmethyl) benzyl] -6-methoxy-4-oxo- 1 , 4 -dihydroqumolme-3 -carboxamide ; N- [3- (N-Benzyl -N-methylammomethyl) benzyl] -6-methoxy-4- oxo-1 , 4 -dihydroqumolme-3 -carboxamide ; N- { 3- [1- (4 -Methylpiperazmyl) methyl] benzyl } -6-methoxy- 4 -oxo-1 , 4 -dihydroqumol e-3 -carboxamide ,- [4 - (2,2, 2-Trιfluoroethy1ammomethyl) benzyl] -6-chloro-4- oxo-1 , 4 -dihydroqumolme-3 -carboxamide ; N-{3-[4- (2 -Pyrimidmyl) piperazmylmethyl] benzyl } -6- methoxy-4 -oxo-1,4-dihydroqumolme-3 -carboxamide ,- N-{3- [1- (1,2,3, 4-Tetrahydroιsoqumolme) methyl] benzyl} - 6 -ethoxy-4 -oxo-1 , 4 -dihydroqumolme-3 -carboxamide; and N- [3- (4 -Morpholmylmethyl) benzyl] -6-fluoro-4-oxo-l , 4- dihydroqumolme-3 -carboxamide . 41. A compound according to claim 1, which is selected from: N- {4- [2- (Ethoxyethoxy) methyl] benzyl} -4-oxo-l, 4- dihydroqumolme-3 -carboxamide; N-{4- [2- (Cyclohexoxyethoxy) methyl] benzyl } -6-ethoxy-4- oxo-1 , 4 -dihydroqumolme-3 -carboxamide ; N- {4- [2- (Phenoxyethoxy) methyl] benzyl } -4-oxo-l , 4- dihydroqumolme-3 -carboxamide ; N- (R) - [3- (1 -Methylaminoethyl) benzyl] -6-chloro-4-oxo- 1 , 4 -dihydroqu olme-3 -carboxamide . 42. A compound according to claim 1, which is N- (S) - [3- (1 -Methylaminoethyl) benzyl] -6-chloro-4-oxo-l , 4- dihydroqumolme- 3 -carboxamide . 43. A compound according to claim 1, which is N- Tetrahydrofurfuryl-4-oxo-l, 4-dιhydro-qumolme-3 - carboxamide . 44. A compound according to claim 1, which is N-{4- [ (N-Methyl -2 -phenethylamino) methyl] benzyl} - 6-ethoxy-4-oxo- 1 , 4 -dιhydroqumolme-3 -carboxamide . 45. A compound according to Claim 1 for use in therapeutic treatment of a disease or disorder associated with pathogenic agonism, inverse agonism or antagonism of the GABA A receptor . 46. A pharmaceutical composition comprising a compound according to Claim 1 combined with at least one pharmaceutically acceptable carrier or excipient. 47. A method for the treatment of a disease or disorder associated with pathogenic associated with pathogenic agonism, inverse agonism or antagonism of the GABA A receptor, said method comprising administering to a patient in need of such treatment or prevention an effective amount of a compound of claim 1. 48. A method according to Claim 47 wherein the disease or disorder associated with pathogenic agonism, inverse agonism or antagonism of the GABA A receptor is anxiety, depression, a sleep disorder, or cognitive impairment. 49. The use of a compound according to Claim 1 for the manufacture of a medicament for the treatment of a disease or disorder associated with pathogenic agonism, inverse agonism or antagonism of the GABA A receptor . 50. The use of a compound according to Claim 1 for the manufacture of a medicament for the treatment of anxiety, depression, sleep disorders, or cognitive impairment. 51. A method for localizing GABA A receptors in a tissue sample comprising: contacting with the sample a detectably- labeled compound of claim 1 under conditions that permit binding of the compound to GABA A receptors, washing the sample to remove unbound compound, and detecting the bound compound. 52. A method of inhibiting the binding of a benzodiazepine compound to a GABA A receptor, said method comprising contacting a compound of claim 1 with cells expressing such a receptor in the presence of the benzodiazepine, wherein the compound is present at a concentration sufficient to inhibit the binding a benzodiazepine compound to a GABA A receptor m vi tro . 53. A method for altering the signal-transducmg activity of GABA A receptors, said method comprising exposing cells expressing such receptors to a compound according to claim 1 at a concentration sufficient to inhibit R015-1788 binding to cells expressing a cloned human GABA A receptor m vi tro . 54. A packaged pharmaceutical composition comprising the pharmaceutical composition of Claim 46 m a container and instructions for using the composition to treat a patient suffering from a disorder responsive to agonism, inverse agonism or antagonism of the GABA A receptor . 55. The packaged pharmaceutical composition of claim 54, wherein said patient is suffering from anxiety, depression, a sleep disorder, or cognitive impairment. 56. A compound according to claim 1 wherein m a standard assay of GABA A receptor binding the compound exhibits an IC 50 of 1 micromolar or less. 57. A compound according to claim 1 wherein in a standard assay of GABA A receptor binding the compound exhibits an IC 50 of 100 nanomolar or less 58. A compound according to claim 1 wherein in a standard assay of GABA A receptor binding the compound exhibits an IC S0 of 10 nanomolar or less.

Inventors: ALBAUGH PAMELA A, CAI GOULIN, CURRIE KEVIN S, ROSEWATER DANIEL, ALBAUGH, PAMELA, A., CAI, GOULIN, CURRIE, KEVIN, S., ROSEWATER, DANIEL
Applicants: ALBAUGH PAMELA A, CAI GOULIN, CURRIE KEVIN S, NEUROGEN CORP, ROSEWATER DANIEL, ALBAUGH, PAMELA, A., CAI, GOULIN, CURRIE, KEVIN, S., NEUROGEN CORPORATION, ROSEWATER, DANIEL
Application year: 2000
Main classification: C07D215
IPC codes: A61K31, A61P25, A61P43, C07D215, C07D401, C07D405, C07D407, C07D409, C07D413, C07D471, C07D487