INHIBITORS OF THE EGF-RECEPTOR TYROSINE KINASE AND THEIR USE

Abstract: Novel compounds and pharmaceutical compositions useful as EGFR tyrosine kinase inhibitors. Methods of the invention include administration of the EGFR TK inhibitors to treat diseases characterized by enhanced expression of EGF, including cancers, particularly breast cancer. Additionally, a homology model representing the structure of EGFR kinase domain is provided, which model is useful for the rationally design and screening of compounds predicted to bind favorably to EGFR and to inhibit EGFR TK.

Description: INHIBITORS OF THE EGF-RECEPTOR TYROSINE KINASE AND THEIR USE Background of the Invention Breast cancer is the most common form of malignancy in women, representing 32% of all new cancer cases and causing 18% of the cancer related deaths among women in the USA . Although the majority of patients with metastatic breast cancer will experience an initial response, survival is only modestly improved with contemporary chemotherapy programs. Consequently, the development of new anti-breast cancer drugs has become a high priority (Abrams, J. S., et al. M. Cancer 1994, 84, 1164). Human epidermal growth factor (EGF) is a 53 amino acid, single-chain polypeptide (Mr 6216 daltons), which exerts biologic effects by binding to a specific cell membrane epidermal growth factor receptor (EGFR / ErbB-1). Many types of cancer cells display enhanced EGFR expression on their cell surface membranes (Khazaie, K., et al. R. B. Cancer & Metasis Reviews 1993, 12, 255). Enhanced expression of the EGFR on cancer cells has been associated with excessive proliferation and metastasis (Mendelsohn, J. and Baselga, J. Biologic Therapy of Cancer: Principles & Practice 1995, 607). Examples include breast cancer, prostate cancer, lung cancer, head and neck cancer, bladder cancer, melanoma, and brain tumors (Khazaie, K., et al. R. B. Cancer & Metasis Reviews 1993, 12, 255). In breast cancer, expression of the EGFR is a significant and independent indicator for recurrence and poor relapse-free survival (Toi, M., et al. European J. Cancer 1991, 27, 977; Chrysogelos, S. A. and Dickson, R. B. Breast Cancer Res. Treat. 1994, 29, 29; Fox, S. B., et al. Breast Cancer Res. Treat. 1994, 29, 41). Additionally, it has been shown that the EGFR has an essential function for the survival of human breast cancer cells (Uckun, F. M., et al. Clin. Can. Res. 1998, 4, 901 ; Moyer, J. D., et al. Cancer Res. 1997, 57(21), 4838). Therefore, the development of PTK inhibitors which abrogate the enzymatic function of the EGFR tyrosine kinase has become a focal point in drug discovery research programs aimed at designing more effective treatment strategies for metastatic breast cancer (George- Nascimento, et al. Biochemistry 1988, 27, 797; Khazaie, K., et al. R. B. Cancer & Metasis Reviews 1993, 12, 255; Fry, D. W. and Bridges, A. J. Curr. Opin. Biotechnol. 1995, 6, 662; Wakeling, A. E., et al. Breast Cancer Research & Treatment 1996, 38, 61). The primary metabolite of the anti-inflammatory leflunomide N-(4- trifluoromethylphenyl)-5-methylisoxazol-4-carboxamide, has been identified as an inhibitor of the EGFR kinase (Parnham, M. J. Exp. Opin Invest. Drugs 1995, 4, 111; Xu, X., et al. Biochem. Pharmacol. 1996, 52, 527; Xu, X., et al. J. Biol. Chem. 1995, 270, 12398; Bertolini, G., et al. J. Mecl. Chem. 1997, 40, 2011; Mattar, T., et al. A. E. E. B. S. Lett. 1993, 334, 161). Despite the identification of this inhibitor of the ΕGFR kinase, however, there is a continuing need for novel anti-cancer drugs. In particular, there is a need for anti-cancer drugs which are more potent or more selective than existing drugs. There is also a need for anti-cancer drugs that operate by novel mechanisms, and thus, may be useful against cancers that do not respond to, or have developed resistance to, existing therapies. Summary of the Invention Applicants have discovered compounds that selectively inhibit ΕGFR tyrosine kinase, without affecting the activity of other PTKs. A representative compound of the invention was also found to inhibit the proliferation and in vitro invasiveness of ΕGFR positive human breast cancer cells at micromolar concentrations. Thus, the compounds of the invention are useful for treating cancer (e.g. breast cancer). The compounds are also useful as pharmacological tools that can be used to further investigate ΕGFR kinase function, or can be used in competitive binding assays to help identify other agents that may be useful as pharmaceuticals. Accordingly, the invention provides a compound of the following formula I: (I) where: R, is (C,-C 3 )alkyl, ( 3 -C 6 )cycloalkyl, phenyl, or NR a R b ; R 2 is hydroxy, (C,-C 6 )alkoxy, or (C,-C 6 )alkanoyloxy; R 3 is cyano, or (C,-C 3 )alkanoyl; R 4 is hydrogen, or (C,-C 3 )alkyl; R 5 is aryl, or heteroaryl; R, and R b are each independently hydrogen, or (C,-C 3 )alkyl; or R a and R b together with the nitrogen to which they are attached are pyrrolidino, piperidino, morpholino, or thiomorpholino; wherein any aryl, or heteroaryl of R, and R. is optionally substituted with one or more (e.g., 1, 2, or 3) substituents independently selected from halo, nitro, cyano, hydroxy, trifluoromethyl, trifluoromethyl, trifluoromethoxy, (C,-C 3 )alkoxy, (C,-C 3 )alkyl, (C,-C 3 )alkanoyl, -S(O) 2 R c , or NR a R b ; wherein R c is (C,-C 3 )alkyl, or aryl or a pharmaceutically acceptable salt thereof. Prefereably, if R 5 is phenyl, the phenyl is substituted by -S(O) 2 R c , or is substituted by halo and at least one other substituent. Preferred compounds of formula I include those of formula II: (II) where R, is (C,-C 6 )alkyl, optionally substituted by 1, 2, or 3 substituents selected from the group consisting of halo, hydroxy, amino, (C,-C 6 )alkoxy, and (C,- C 6 )alkanoyloxy; R 2 is hydroxy, (C,-C 6 )alkoxy, (C,-C 6 )alkanoyloxy;R 3 is cyano, alkanoyl; R 6 is amino, hydroxy, cyano, nitro, (C,-C 6 )alkyl, halo(C,-C 6 )alkyl, (C,- C 6 )alkoxy, halo(C,-C 6 )alkoxy, (C,-C 6 )alkanoyl, or (C t -C 6 )alkanoyloxy; and R 7 is H, NH 2 , CH 3 , OH, CF 3 , or halo, preferably, halo is Br or Cl; or a pharmaceutically acceptable salt thereof. Particularly compounds of formula I include those of formulae III- VI: (HI) where R 7 is H, NH 2 , CH 3 , OH, CF 3 , or halo. Preferably, halo is Br or Cl. (IV) where R 7 is H, NH 2 , CH 3 , OH, CF 3 , or halo. Preferably, halo is F or Cl. (V) where R 6 is NH-CH 3 or OCH 3 . (VI) where R 2 is -CH,-CH 2 X and X is halo, preferably F; or R 2 is -CH 2 CF 3 ; or R 2 is: -N O The invention also provides a pharmaceutical composition comprising a compound of formula I; or a pharmaceutically acceptable salt thereof; and a pharmaceutically acceptable carrier. A particularly useful compound of the invention is LFM 12, having the structural formula: The invention also provides a therapeutic method for treating diseases in which EGFR is overexpressed, particularly cancers (e.g. breast cancer) comprising administering to a mammal in need of such therapy, a compound of the invention, e.g., of formulae I- VI; or a pharmaceutically acceptable salt thereof. The invention also provides a compound for use in medical therapy (preferably for use in treating cancer), as well as the use of a compound of formulae I- VI for the manufacture of a medicament for the treatment of a pathological condition or symptom in a mammal, such as a human, which is associated cancer (e.g. breast cancer). The invention also provides a homology model representing the structure of the EGF-R kinase domain and a docking procedure, which are useful to rationally design compounds predicted to bind favorably to EGF-R and inhibit EGFR-TK activity. Using this model, leflunomide metabolite analogs were designed and found to have potent inhibitory activity against EGFR TK (IC 5 0 value of 1.7μM in EGF-R inhibition assays, killing >99%o of human breast cancer cells in vitro by triggering apoptosis). New potent LFM analogs as active inhibitors of the EGF-R tyrosine kinase are designed and confirmed using this model. The invention also provides processes and novel intermediates, described herein, that are useful for preparing compounds of formulae I, II, IV- VI. Brief Description of the Figures Figures 1A-1C show a homology model fo the EGF-R kinase domain. Figure 1 A is a molecular surface model showing the plan as active site. Figures IB and 1C are stereo views of the catalytic site of the EGF-R kinase domain with the inhibitor binding region represented as a triangular- shaped grid. Figures 2A-2B are photographs showing the homology model of the EGFR kinase domain. Figure 2 A is a ribbon (Cα chain) representation of the homology model of the EGFR kinase domain and a space filling model of the compound LFM- A12 which was docked into the catalytic site. The N-lobe shown in blue is primarily composed of β-sheets and the C-lobe shown in gray is mostly helical. The hinge region is shown in peach. Prepared using Molscript and Raster3D programs (Bacon, D. J. and Anderson, W. F. J. Molec. Graphics 1988, , 219; Kraulis, P. J. Appl. Cryst. 1991, 24, 946; Merritt, E. A. and Murphy, M. E. P. Ada Cryst. 1994, D50, 869). Figure 2B is a space filling representation of the catalytic site residues of the EGFR kinase domain. The Cα chain of EGFR is represented as a pink ribbon and the residues comprising the hinge region are shown in blue. One corner of the triangular binding region is located between the T766 (peach) and D831 (lavender) residues. The second corner bordering the binding region is located at R817 shown in green and the third corner is near the lower left side of the hinge region. A ball and stick model of the inhibitor LFM-A12 is shown in multicolor and represents a favorable orientation of this molecule in the kinase active site of EGFR. Prepared using Insightll program (Insightll, Molecular Simulations Inc. 1996, San Diego, CA). Figure 3 shows the superimposed docked positions of ihe para substituted LFM analogs at the active site of the EGFR tyrosine kinase. The residues in the active site are shown in pink. LFM: para-CV 3 substituted compound (active metabolite of leflunomide, green); LFM-Al : /.αr-.-Br substituted analog (blue); LFM-A2: para-Cλ substituted analog (red); LFM- A3 : p ara-Y substituted LFM analog (white). LFM-A12: para-OCF 3 substituted LFM analog (gold). Figure 4 shows the superimposed docked positions of CF 3 substituted LFM analogs in the active site of the EGFR tyrosine kinase. The residues in the active site are shown in pink. LFM: para-C¥ 3 substituted compound (active metabolite of leflunomide, green); LFM-A8: meta-CF substituted analog (yellow); LFM-A4: ortho-C¥ 3 substituted analog (red). Figures 5A-5F are anti-phosphotyrosine Western blots showing selective inhibition of EGFR tyrosine kinase by LFM-A12. Figure 5A shows EGFR immune complexes from lysates of MDA-MB-231 human breast cancer cells treated with LFM or LFM-A12 for 1 hour and then assayed for tyrosine kinase activity, as described in Example 5. Figure 5B shows a lack of inhibition of IRK immunoprecipitated from HepG2 hepatoma cells in immune complex kinase assays after treatment with LFM or LFM-A12. Figure 5C shows a lack of inhibition of HCK immunoprecipitated from transfected COS7 cells in immune complex kinase assays after treatment with LFM or LFM-A12. Figures 5D-5F show a lack of inhibition of JAK3, JAK1, and BTK immunoprecipitated from lysates of transfected insect ovary cells. Figures 6A-6B show the docking of LFM-A12 and WHI-P 154 into catalytic sites of kinases. Figure 6A shows the superimposed backbones of the catalytic site residues of the kinase domain homology models of EGFR (white), PTK (peach) and crystal structure coordinates of HCK (blue), with selected residues at positions A, B, and C. LFM-A12 is shown in multicolor and represents its docked positionin BTK, which is also similar to its docked position in HCK. The white dotted surface area represents the Connolly surface of LFM-A12. Figure 6B shows superimposed backbones of the catalytic site residues of the kinase domain homology models of EFGR (white), JAK3 (pink) and crystal structure coordinates of IRK (green), with selected residues at positions A, B, and C. LFM-A12 is shown in multicolor and represents its docked positionin EGFR which is also similar to its docked position in IRK and JAK3. The white dotted surface area represents the Connolly surface of LFM-A12. Figures 7A-7B demonstrates anti-invasive activity of LFM-A12 against MDA-MB-231 human breast cancer cells. Cells were incubated with the indicated concentrations of LFM or LFM-A12 for 24 hours, trypsinized, and placed in Boyden chambers precoated with Matrigel matrix and allowed to migrate for 48 hours. The migrated cells were stained with Hema II solution and counted. The data points are the mean values from two independent experiments. Untreated breast cancer cells were highly invasive in Matrigel-coated Boyden chambers. Figure 7A is a bar graph showing the invasion of LFM-A12-treated breast cancer cells through the Matrigel matrix was inhibited in a dose-dependent fashion. The invasion of LFM-treated breast cancer cells was inhibited to a lesser extent. The mean IC 50 values were 28.4 μM for LFM-A12 and 97.0 μM for LFM. Figure 7B is a series of photographs showing microscopic evidence for the dose-dependent reduction of the numbers of migrated MDA-MB-231 cells after treatment with LFM-A12. Figures 8A-8B are graphs showing the cytotoxic activity of LFM and LFM- A12 against human breast cancer cells in MTT assays. MDA-MB-231 and MDA- MB-361 cells were treated with LFM (Figure 8A) or LFM-A12 (Figure 8B) for 36 hours in 96-well plates and the cytotoxicity was determined by the MTT assay. The data points represent the mean (± SE) values from three independent experiments. Figures 9A-9C are confocal images of LFM-A12-treated breast cancer cells. MDA B-231 cells were treated with LFM- A3 or LFM-A12 at a final concentration of 100 μM for 24 hours at 37°C, as described in the Examples. After treatment, cells were processed for immunofiuorescence using a monoclonal antibody to α-tubulin (green fluorescence). LFM-A12 (Figure 9A) but not LFM-A3 (Figure 9C) -treated cells showed marked shrinkage with disruption of microtubules and lost their ability to adhere to the substratum. Blue fluorescence represents nuclei stained with TOTO- 3. The control is shown in Figure 9A Figures 10A and 10B diagramatically show regions of LFM-A12 suitable for modificatin to enhance EGFR inhibition. Structural and chemical features of LFM analogs which are proposed to aid binding to the EGFR catalytic site and are described below and illustrated. Binding Mode 1, (Figure 10A) shows the most likely mode of binding of the lead compound LFM-A12 at the EGFR catalytic site. Based on the modifications of the lead compound, a second mode may also be possible and this is illustrated in Figure 10B (Binding Mode 2). Detailed Description of the Invention The following definitions are used, unless otherwise described: halo is fluoro, chloro, bromo, or iodo. Alkyl, alkoxy, etc. denote both straight and branched groups; but reference to an individual group such as "propyl" embraces only the straight chain group, a branched chain isomer such as "isopropyl" being specifically referred to. It will be appreciated by those skilled in the art that compounds of the invention having a chiral center may exist in and be isolated in optically active and racemic forms. Some compounds may exhibit polymorphism. It is to be understood that the present invention encompasses any racemic, optically-active, polymorphic, or stereoisomeric form, or mixtures thereof, of a compound of the invention, which possess the useful properties described herein, it being well known in the art how to prepare optically active forms (for example, by resolution of the racemic form by recrystalhzation techniques, by synthesis from optically-active starting materials, by chiral synthesis, or by chromatographic separation using a chiral stationary phase) and how to determine a compounds ability to inhibit EGFR tyrosine kinase using the standard tests described herein, or using other similar tests which are well known in the art. Specific and preferred values listed below for substituents and ranges, are for illustration only; they do not exclude other defined values or other values within defined ranges for the substituents. The term "prodrug moiety" is a substitution group which facilitates use of a compound of the invention, for example by facilitating entry of the drug into cells or administration of the compound. The prodrug moiety may be cleaved from the compound, for example by cleavage enzymes in vivo. Examples of prodrug moieties include phosphate groups, peptide linkers, and sugars, which moieties can be hydro lized in vivo. Compounds of the Invention Specific inhibitors of EGFR tyrosine kinase include those of formula I: (I) where: R, is (C,-C 3 )alkyl, ( 3 -C 6 )cycloalkyl, phenyl, or NR a R b ; R 2 is hydroxy, (C r C 6 )alkoxy, or (C,-C 6 )alkanoyloxy; R 3 is cyano, or (C r C 3 )alkanoyl; R 4 is hydrogen, or (C r C 3 )alkyl; R 5 is aryl, or heteroaryl; R t and R b are each independently hydrogen, or (C,-C 3 )alkyl; or R a and R b together with the nitrogen to which they are attached are pyrrolidino, piperidino, morpholino, or thiomorpholino; wherein any aryl, or heteroaryl of R, and R 5 is optionally substituted with one or more (e.g., 1, 2, or 3) substituents independently selected from halo, nitro, cyano, hydroxy, trifluoromethyl, trifluoromethyl, trifluoromethoxy, (C,-C 3 )alkoxy, (C,-C 3 )alkyl, (C,-C 3 )alkanoyl, -S(O) 2 R c , or NR a R b ; wherein R c is (C,-C 3 )alkyl, or aryl or a pharmaceutically acceptable salt thereof; (II) Preferred are compounds of formula II, where R, is (C,-C 6 )alkyl, optionally substituted by 1, 2, or 3 substituents selected from the group consisting of halo, ΔΔΔ Δ, (C,-C 6 )alkoxy, R 2 is hydroxy, (C,-C 6 )alkoxy, (C,-C 6 )alkanoyloxy; R 3 is cyano, alkanoyl; R 4 is amino, hydroxy, cyano, nitro, (C,-C 6 )alkyl, halo(C,-C 6 )alkyl, (C,- C 6 )alkoxy, halo(C,-C 6 )alkoxy, (C,-C 6 )alkanoyl, or (C,-C 6 )alkanoyloxy; and R 5 is H, NH 2 , CH 3 , OH, CF 3 , or halo, preferably halo is Br or Cl; or a pharmaceutically acceptable salt thereof. Specifically, (C,-C 6 )alkyl can be methyl, ethyl, propyl, isopropyl, butyl, iso- butyl, sec-butyl, pentyl, 3-pentyl, or hexyl; (C,-C 6 )alkoxy can be methoxy, ethoxy, propoxy, isopropoxy, butoxy, iso-butoxy, sec-butoxy, pentoxy, 3-pentoxy, or hexyloxy; (C,-C 6 )alkanoyl can be acetyl, propanoyl or butanoyl; halo(C,-C 6 )alkyl can be iodomethyl, bromomethyl, chloromethyl, fluoromethyl, trifluoromethyl, 2- chloroethyl, 2-fluoroefhyl, 2,2,2-trifluoroethyl, or pentafluoroethyl; and (C 2 - C 6 )alkanoyloxy can be acetoxy, propanoyloxy, butanoyloxy, isobutanoyloxy, pentanoyloxy, or hexanoyloxy. A specific value for R, is (C,-C 6 )alkyl, optionally substituted by halo, hydroxy, amino, or (C,-C 6 )alkoxy. Another specific value for R } is (C,-C 6 )alkyl. A preferred value for R, is methyl. A preferred value for R 2 is hydroxy. A preferred value for R 3 is cyano. A preferred value for R 4 is trifluoromethoxy. A preferred compound is a compound of formula I wherein R, is (C,- C 6 )alkyl; R 2 is hydroxy, (C,-C 6 )alkoxy, or (C,-C 6 )alkanoyloxy; R 3 is cyano; and R 4 trifluoromethoxy; or a pharmaceutically acceptable salt thereof. Another preferred compound is a compound of formula I wherein R, is methyl; R 2 is hydroxy; R 3 is cyano; and R 4 trifluoromethoxy; or a pharmaceutically acceptable salt thereof. Preferred compounds of the invention include novel analogs of LFM designed to better fit the EGFR binding pocket and better interact with amino acid residues forming contacts within the pocket. These compounds of the invention fall within four groups, or types, and are more fully described below in Example 6. These compounds have the following structural formulae (III- VI): (III) where R 5 is H, NH 2 , CH 3 , OH, CF 3 , or halo. Preferably, halo is Br or Cl. (IV) where R 5 is H, NH 2 , CH 3 , OH, CF 3 , or halo. Preferably, halo is F or Cl. (V) where R 4 is NH-CH 3 or OCH-. (VI) where R, is -CH 2 -CH 2 X and X is halo, preferably F; or -N O R, is: Processes for preparing compounds of formulae I-VI are provided as further embodiments of the invention and are illustrated by the following procedures and Examples in which the meanings of the generic radicals are as given above unless otherwise qualified. A compound of formula I wherein R 2 is hydroxy, can conveniently be prepared by treating an intermediate of formula A: with an acid chloride of formula: R,COCl. (A) In cases where compounds are sufficiently basic or acidic to form stable nontoxic acid or base salts, administration of the compounds as salts may be appropriate. Examples of pharmaceutically acceptable salts are organic acid addition salts formed with acids which form a physiological acceptable anion, for example, tosylate, methanesulfonate, acetate, citrate, malonate, tartarate, succinate, benzoate, ascorbate, ketoglutarate, and glycerophosphate. Suitable inorganic salts may also be formed, including hydrochloride, sulfate, nitrate, bicarbonate, and carbonate salts. Pharmaceutically acceptable salts may be obtained using standard procedures well known in the art, for example by reacting a sufficiently basic compound such as an amine with a suitable acid affording a physiologically acceptable anion. Alkali metal (for example, sodium, potassium or lithium) or alkaline earth metal (for example calcium) salts of carboxylic acids can also be made. The compounds of the invention may have attached thereto functinal groups to provide a prodrug derivative. The prodrug deriviative facilitates use of the drug in the body, for example, by facilitating entry into cells. The prodrug derivative may be cleaved or not in the active compound. Pharmaceutical Compositions The compounds of formula I can be formulated as pharmaceutical compositions and administered to a mammalian host, such as a human patient in a variety of forms adapted to the chosen route of administration, i.e., orally or parenterally, by intravenous, intramuscular, topical or subcutaneous routes. Thus, the present compounds may be systemically administered, e.g., orally, in combination with a pharmaceutically acceptable vehicle such as an inert diluent or an assimilable edible carrier. They may be enclosed in hard or soft shell gelatin capsules, may be compressed into tablets, or may be incorporated directly with the food of the patient's diet. For oral therapeutic administration, the active compound may be combined with one or more excipients and used in the form of ingestible tablets, buccal tablets, troches, capsules, elixirs, suspensions, syrups, wafers, and the like. Such compositions and preparations should contain at least 0.1% of active compound. The percentage of the compositions and preparations may, of course, be varied and may conveniently be between about 2 to about 60% of the weight of a given unit dosage form. The amount of active compound in such therapeutically useful compositions is such that an effective dosage level will be obtained. Conjugation to a Targeting Moiety The compounds of the invention may be conjugated to a targeting moiety for targeted delivery to a desired cell. Useful targeting moieties are antibodies or specific ligands that bind to an antigen or ligand receptor on the specific target cells. For example, the EGFR TK inhibitors of the invention may be targeted to EGFR- expressing cells by conjugation of the inhibitor to an anti-EGFR antibody, or by conjugation to EGF. Other useful targeting moieties include molecules which bind or associate with the EGFR, including TGF-alpha, Erb2, Erb3, Erb4, or antibodies against these molecules. To form the conjugates of the invention, a targeting moiety, which is often a polypeptide molecule, is bound to the compounds of the invention at reactive sites, including NH 2 , SH, CHO, COOH, and the like. Specific linking agents are used to link the compounds. Preferred linking agents are chosen according to the reactive site to which the targeting moiety is to be attached. Methods for selecting an appropriate linking agent and reactive site for attachment of the targeting moiety to the compound of the invention are known, and are described, for example, in Hermanson, et.al., Bioconjugate Techniques, Academic Press, 1996; Hermanson, et.al., Immobilized Affinity Ligand Techniques, Academic Press, 1992; and Ezerce Catalog and Handbook, 1996, ppT155-T201. Pharmaceutical additives The tablets, troches, pills, capsules, and the like may also contain the following: binders such as gum tragacanth, acacia, corn starch or gelatin; excipients such as dicalcium phosphate; a disintegrating agent such as corn starch, potato starch, alginic acid and the like; a lubricant such as magnesium stearate; and a sweetening agent such as sucrose, fructose, lactose or aspartame or a flavoring agent such as peppermint, oil of wintergreen, or cherry flavoring may be added. When the unit dosage form is a capsule, it may contain, in addition to materials of the above type, a liquid carrier, such as a vegetable oil or a polyethylene glycol. Various other materials may be present as coatings or to otherwise modify the physical form of the solid unit dosage form. For instance, tablets, pills, or capsules may be coated with gelatin, wax, shellac or sugar and the like. A syrup or elixir may contain the active compound, sucrose or fructose as a sweetening agent, methyl and propylparabens as preservatives, a dye and flavoring such as cherry or orange flavor. Of course, any material used in preparing any unit dosage form should be pharmaceutically acceptable and substantially non-toxic in the amounts employed. In addition, the active compound may be incorporated into sustained-release preparations and devices. The active compound may also be administered intravenously or intraperitoneally by infusion or injection. Solutions of the active compound or its salts can be prepared in water, optionally mixed with a nontoxic surfactant. Dispersions can also be prepared in glycerol, liquid polyethylene glycols, triacetin, and mixtures thereof and in oils. Under ordinary conditions of storage and use, these preparations contain a preservative to prevent the growth of microorganisms. The pharmaceutical dosage forms suitable for injection or infusion can include sterile aqueous solutions or dispersions or sterile powders comprising the active ingredient which are adapted for the extemporaneous preparation of sterile injectable or infusible solutions or dispersions, optionally encapsulated in liposomes. In all cases, the ultimate dosage form should be sterile, fluid and stable under the conditions of manufacture and storage. The liquid carrier or vehicle can be a solvent or liquid dispersion medium comprising, for example, water, ethanol, a polyol (for example, glycerol, propylene glycol, liquid polyethylene glycols, and the like), vegetable oils, nontoxic glyceryl esters, and suitable mixtures thereof. The proper fluidity can be maintained, for example, by the formation of liposomes, by the maintenance of the required particle size in the case of dispersions or by the use of surfactants. The prevention of the action of microorganisms can be brought about by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, sorbic acid, thimerosal, and the like. In many cases, it will be preferable to include isotonic agents, for example, sugars, buffers or sodium chloride. Prolonged absorption of the injectable compositions can be brought about by the use in the compositions of agents delaying absorption, for example, aluminum monostearate and gelatin. Sterile injectable solutions are prepared by incorporating the active compound in the required amount in the appropriate solvent with various of the other ingredients enumerated above, as required, followed by filter sterilization. In the case of sterile powders for the preparation of sterile injectable solutions, the preferred methods of preparation are vacuum drying and the freeze drying techniques, which yield a powder of the active ingredient plus any additional desired ingredient present in the previously sterile-filtered solutions. For topical administration, the present compounds may be applied in pure form, i.e., when they are liquids. However, it will generally be desireable to administer them to the skin as compositions or formulations, in combination with a detematology acceptable carrier, which may be a solid or a liquid. Useful solid carriers include finely divided solids such as talc, clay, microcrystalline cellulose, silica, alumina and the like. Useful liquid carriers include water, alcohols or glycols or water-alcohol/glycol blends, in which the present compounds can be dissolved or dispersed at effective levels, optionally with the aid of non-toxic surfactants. Adjuvants such as fragrances and additional antimicrobial agents can be added to optimize the properties for a given use. The resultant liquid compositions can be applied from absorbent pads, used to impregnate bandages and other dressings, or sprayed onto the affected area using pump-type or aerosol sprayers. Thickeners such as synthetic polymers, fatty acids, fatty acid salts and esters, fatty alcohols, modified celluloses or modified mineral materials can also be employed with liquid carriers to form spreadable pastes, gels, ointments, soaps, and the like, for application directly to the skin of the user. Examples of useful dermatological compositions which can be used to deliver the compounds of formula I to the skin are known to the art; for example, see Jacquet et al. (U.S. Pat. No. 4,608,392), Geria (U.S. Pat. No. 4,992,478), Smith et al. (U.S. Pat. No. 4,559,157) and Wortzman (U.S. Pat. No. 4,820,508). Treatment Methods The EGFR-tyrosine kinase inhibitors of the invention are useful to inhibit the activity of EGFR- tyrosine kinasae. In particular, the compounds are usful in the treatment of diseases and pathological conditions that involve cells expressing EGFR. Most importantly, the inhibitors of the present invention provide a treatment for selectively inhibiting EGFR tyrosine kinase, without significant inhibition of other tyrosine kinases, such as the Src family, Tec family, and Janus family tyrosine kinases. Many known pathologic conditions involve the EGFR and the activity of the EGFR tyrosine kinase. These include cancers such as breast cancer, prostate cancer, lung cancer, brain tumor, bladder cancer, and colon cancer. The EGFR TK inhibitors of the invention are delivered to the EGFR, and particularly to the kinase binding domain of the receptor, in order to inhibit the kinase activity. The compounds of the invention may be administered to cancer patients to treat exsisting cancer, or be administered to those at risk for developing cancer, including genetic, occupational, and nutritional predisposition to cancer. Additional disorders known to involve EGFR and its TK include atherosclerosis, disorders of vascular smooth muscle cells, and endothelial cells involved in tumor angiogenesis. Dosage Useful dosages of the compounds of formula I can be determined by comparing their in vitro activity, and in vivo activity in animal models. Methods for the extrapolation of effective dosages in mice, and other animals, to humans are known to the art; for example, see U.S. Pat. No. 4,938,949. Generally, the concentration of the compound(s) of formula I in a liquid composition, such as a lotion, will be from about 0.1-25 wt-%, preferably from about 0.5-10 wt-%. The concentration in a semi-solid or solid composition such as a gel or a powder will be about 0.1-5 wt-%, preferably about 0.5-2.5 wt-%. The amount of the compound, or an active salt or derivative thereof, required for use in treatment will vary not only with the particular salt selected but also with the route of administration, the nature of the condition being treated and the age and condition of the patient and will be ultimately at the discretion of the attendant physician or clinician. In general, however, a suitable dose will be in the range of from about 0.5 to about 100 mg/kg, e.g., from about 10 to about 75 mg/kg of body weight per day, such as 3 to about 50 mg per kilogram body weight of the recipient per day, preferably in the range of 6 to 90 mg/kg/day, most preferably in the range of 15 to 60 mg/kg/day. The compound is conveniently administered in unit dosage form; for example, containing 5 to 1000 mg, conveniently 10 to 750 mg, most conveniently, 50 to 500 mg of active ingredient per unit dosage form. Ideally, the active ingredient should be administered to achieve peak plasma concentrations of the active compound of from about 0.5 to about 75 μM, preferably, about 1 to 50 μM, most preferably, about 2 to about 30 μM. This may be achieved, for example, by the intravenous injection of a 0.05 to 5% solution of the active ingredient, optionally in saline, or orally administered as a bolus containing about 1- 100 mg of the active ingredient. Desirable blood levels may be maintained by continuous infusion to provide about 0.01-5.0 mg/kg/hr or by intermittent infusions containing about 0.4-15 mg/kg of the active ingredient(s). The desired dose may conveniently be presented in a single dose or as divided doses administered at appropriate intervals, for example, as two, three, four or more sub-doses per day. The sub-dose itself may be further divided, e.g., into a number of discrete loosely spaced administrations; such as multiple inhalations from an insufflator or by application of a plurality of drops into the eye. The invention may be better understood with reference to the following Examples, which are not intended to limit the scope of the invention. Example 1 Crystal Structures of Leflunomide Metabolite and Its Analogs Small molecule X-ray crystal structures were obtained for 10 of the 15 leflunomide metabolite analogs studied herein. The compounds were crystallized using various solvents by evaporation, vapor diffusion or liquid-liquid diffusion. X- ray data on single crystals were collected on a SMART CCD detector (Bruker Analytical X-ray Systems, Madison, WI) using MoKα radiation (λ= 0.7107 A). The space group for each crystal was determined based on systematic absences and intensity statistics. A direct methods solution provided most of the non-hydrogen atoms from the electron density map. Several full-matrix least squares/difference Fourier cycles were performed to locate the remaining non-hydrogen atoms. All non-hydrogen atoms were refined with anisotropic thermal parameters. Hydrogen atoms were placed in ideal positions and refined as riding atoms with relative isotropic temperature factors. The structure was refined using full-matrix least- squares data on F 2 . Crystal structure calculations were performed using a Silicon Graphics INDY R4400-SC computer (Silicon Graphics Inc., Mountain View, CA) or Pentium computer using the SHELXTL V 5.0 (Sheldrick, G. SHELXTL Bruker Analytical X-ray Systems, Madison, WI) suite of programs. The crystal data, experimental parameters, and refinement statistics are summarized in Table 1. Table 1. Crystal data, data collection and refinement statistics for leflunomide metabolite analogs. Table 1 continued Example 2 Construction of a Homology Model for the EGF Receptor Kinase Domain A homology model for the EGFR kinase domain was constructed based on a structural alignment of the sequence of EGFR (accession # P00533, SWISS-PROT, Univ. of Geneva, Geneva, Switzerland) obtained from Genbank (National Center for Biotechnology Information, Bethesda, MD) with the sequences of known crystal structures of other protein kinases (kinase domains of HCK (Sicheri, F., et al. Nature 1997, 355, 602), FGFR (Mohammadi, M., et al. Science 1997, 276, 955), IRK (Hubbard, S. R. The E. M. B. O. Journal 1997, 16, 5572), and cAPK (Zheng, J., et al. Ada Cryst. 1993, D49, 362)). A multiple sequence alignment of HCK, FGFR, IRK, and cAPK with that of EGFR was carried out manually, conserving the overall secondary structure within the family. Once the correspondence between amino acids in the reference and model sequences were made, the coordinates for the structurally conserved regions were assigned based on the coordinates of the reference proteins. Insertions, deletions and mutations were incorporated into the template structure to build an initial model. The final model of EGFR was subjected to energy minimization to refme the molecular structure so that any steric strain introduced during the model- building process could be relieved (Brunger, A. T. X-PLOR 1992, New Haven, CT). The model was screened for unfavorable steric contacts and, if necessary, such side chains were remodeled either by using a rotamer library database or by manually rotating the respective side chains. The modeling was carried out on a Silicon Graphics INDIGO2 computer (Silicon Graphics Inc., Mountain View, CA) using the Homology module in INSIGHTII (Insightll, Molecular Simulations Inc. 1996, San Diego, CA). The final homology model of the EGFR kinase domain had an rms deviation of 0.006 A from ideal bond lengths and 2.0° from ideal bond angles after energy minimization. The above procedure was used to construct the homology model, and the homology model was used, in conjunction with small „ , 24 molecule crystal structures of the leflunomide analogs, for modeling studies of the EGF-R/LFM complexes. The modeled EGFR kinase domain has the expected protein kinase fold with the catalytic site in the center dividing the kinase domain into two lobes. It is composed of a smaller N-terminal lobe connected by a flexible hinge (residues 764 to 773) to a larger C-terminal lobe (See Figures 1 A-1B and 2A-2C). The N-terminal lobe is rich in β-strands, while the C-terminal lobe is mostly helical. The catalytic site is defined by two β-sheets that form an interface at the cleft between the two lobes. The catalytic site of the EGFR kinase domain displays a remarkably planar triangular binding pocket, which can bind the base ring portion of ATP. The sides of this triangle are approximately 15A x 12A x 12A and the thickness of the binding pocket is approximately 7 A, with an estimated volume of approximately 600 A 3 . The characteristics of this triangular region, which binds the base ring portion of ATP, was elucidated using a binding sphere surface calculated by the program SPHGEN in DOCK3.5. (Kuntz, I.D. et al., J.Mol. Biol, 1982: 161 : 269-288). Two sides of the triangle can be visualized as beginning at an apex located between Thr 766 (peach residue in Figure IB) and Asp 831 (lavender residue in Figure IB), and extending towards the solvent-accessible opening of the catalytic site. One side of the triangle extends from the apex along the hinge region of the catalytic site (blue residues in Figure IB), and a second side extends from the apex to Arg 817 (green residue in Figure IB) which is immediately adjacent to the binding subsites for the sugar and triphosphate groups of ATP. The hinge region of the binding site is composed of residues 764 to 773 (Figure 2). The third side of the triangle extends along the slot-shaped opening to the catalytic site. The crystal structures of the HCK/quercetin complex (Sicheri, F., et al. Nature 1997, 385, 602) and two FGFR/inhibitor complexes (Mohammadi, M., et al. Science 1997, 276, 955) revealed that the reported three inhibitors of HCK and FGFR bind to the catalytic sites of the respective tyrosine kinases. When the catalytic sites are superimposed with EGFR, all atoms of the three PTK inhibitors fall within the plane of the triangle described previously, and each molecule is in close contact with the superimposed hinge region and Asp 831 of EGFR. Moreover, they characteristically occupy only half of the triangle, near the hinge region. This molecular fitting feature seems to correlate with tighter binding and may be an important determinant for effective inhibitor binding. Similarly, the size and planar shape of the catalytic site within the constructed EGFR kinase domain are likely to contribute to its ability to form energetically favorable interactions with planar molecules such as the LFM analogs described herein. These considerations are in agreement with conclusions derived from the structure-activity relationship analyses of pyrolo- and pyrazoloquinazoline compounds (Palmer, B. D. et al. J. Mecl. Chem. 1997, 40, 1519) and were therefore incorporated into the modeling strategy. While most of the catalytic site residues of the EGFR kinase domain were conserved relative to other PTKs, we noted a few specific variations. EGFR residues Leu 694 , Val 702 , Lys 721 , and Ala 719 are conserved in EGFR, HCK, FGFR and IRK. Residues Asn 818 and Asp 831 (opposite to the hinge) are conserved in EGFR, HCK, FGFR, IRK, BTK, JAKl, and JAK3. Residues Cys 751 and Thr 830 are specific for EGFR but vary in BTK (Val, Ser), JAKl (Val, Gly), JAK3 (Val, Ala), IRK (Val, Gly), and HCK (Val, Ala). Residues Thr 766 and Leu 768 in the hinge region changes to Met and Leu in IRK, Met and Phe in JAKl , Met and Tyr in JAK3, and to Thr and Tyr in BTK. The right side of the binding pocket (Figures 2 - 4) contains Cys 773 in EGFR and is therefore considerably more hydrophobic than the corresponding residue of PDGFR (Asp), FGFR (Asn), JAKl (Ser), HCK (Ser), and IRK (Asp). These residue differences provide a basis for designing selective inhibitors of the EGFR kinase domain. Thus, the catalytic site of the EGFR kinase has a triangular shaped binding region which contains some specific nonconserved residues. This information, combined with the homology model developed and described above, can be used to design compounds that will possess activity as EGFR kinase inhibitors. Selectivity for a particular tyrosine kinase can also be achieved by extending the binding area to the other half of the triangle (opposite the hinge), as has been observed in one of the FGF-R inhibitor structures (Mohammadi, M., et al. Cell 1996: 86, 577-87) and (Connolly, M.L., Science, 1983, 221 : 709-13). The difference in binding affinity is provided by Phe489 which extends from a beta hairpin toward n£ 26 the inhibitor. EGF-R has a shorter and more rigid beta hairpin that FGFR which would present some limitations to this strategy. The non-conserved residue Arg 817 could also provide some binding discrimination if the inhibitor binds nearby. We concluded from our modeling studies that the catalytic site of the EGF-R has specific features which can be advantageous for the design of inhibitors. These features include a triangular shaped region which is accessible to an inhibitor. We hypothesized that molecules fitting the triangular shape of the EGF-R catalytic site which can also form favorable contacts with the hinge region of the binding site will bind more strongly and hence inhibit EGF-R more effectively. In order to elucidate the structure activity relationships determining the ability of LFM to inhibit the EGF-R tyrosine kinase as well as test the predictive value of our homology model of the EGF-R kinase domain, we have designed and synthesized analogs of this compound by systematically replacing the 7-CF 3 substituent in the phenyl ring. Our modeling calculations were based on the homology model of the EGF-R kinase domain described above and crystal structures of the LFM and its analogs. The positions of the critical residues in the active site of the EGF-R and the docked positions of the LFM analogs are shown in Figures 2-3. The docked positions of the compounds indicate that the molecules maintain a close contact with the hinge region. In all cases the nitrile nitrogen of the ligand was involved in hydrogen bonding with the amide NH of Met. In modeling the inhibitory activity of LFM analogs with EGF-R tyrosine kinase, we calculated the binding constants (Kj values) based on the binding interaction between the compounds and the catalytic site of the EGF-R kinase domain. Example 3 Structure-Based Design and Synthesis of LFM Analogs Having Potent EGFR-Inhibitory Activity We hypothesized that molecules fitting the triangular shape of the EGFR catalytic site which can also form favorable contacts with the hinge region of the binding site would bind more strongly and hence inhibit the EGFR kinase more effectively. In modeling studies aimed at identifying LFM analogs with a high likelihood to bind favorably to the catalytic site of the EGFR kinase domain, we chose to calculate the K, values based on the binding interaction between the inhibitor and EGFR residues. The K, values were calculated for several different inhibitors and were used to rank the predicted binding strength. Each of the small molecule LFM analogs described below was individually modeled into the catalytic site of the EGFR kinase domain using an advanced docking procedure. The position of quercetin in the HCK crystal structure (Sicheri, F., et al. Nature 1997, 385, 602) was used as a template to obtain a reasonable starting point for the docking procedure. The various docked positions of each LFM analog was qualitatively evaluated and consequently compared with the IC 50 values of the compounds in cell-free EGFR kinase inhibition assays. Table 2 lists the interaction scores and calculated K, values for LFM and its analogs. Table 2. Interaction scores, estimated K, values and measured IC 50 values for LFM analogs. ΗB = Number of hydrogen bonds. b Ludι K, calculated based on the empirical score function in Ludi program(Bohm, H. J. J Comput Aided Mol Des 1992, 6, 593; and 36. Bohm, H. J. J Comput Aided Mol Des 1994, 8, 243) Cell-free EGF R kinase assays and MTT assays were performed as described in the Methods and the corresponding IC50 values were calculated from the dose-dependent inhibition curves. Docking Procedure and Evaluation of Protein-inhibitor Interactions: Fixed docking in the Affinity program within Insightll (Insightll, Molecular Simulations Inc. 1996, San Diego, CA) was used for docking the LFM analogs to the EGFR tyrosine kinase catalytic site. A triangular binding region, where the base ring of ATP can bind to EGFR, was defined using a binding sphere surface calculated by the program SPHGEN in DOCK3.5 (Kuntz, I. D., et al. J. Mol. Biol. 1982, 161, 269). The modeling calculations used to study the predicted binding of inhibitors to EGFR were based on the homology model of EGFR described above and the coordinates of energy-minimized models of the compounds which were used for docking. The utility of the inhibitor model coordinates were validated by their crystal structures which were obtained later and showed very similar molecular conformations. Each LFM analog was interactively docked into the triangular binding pocket of EGFR based on the position of quercetin in the HCK/quercetin crystal structure. The hydrogens on the EGFR were generated and potentials were assigned to both receptor and ligand prior to the start of the docking procedure. The docking method in the Insightll program uses the CVFF force field and a Monte Carlo search strategy to search for and evaluate docked structures (Insightll, Molecular Simulations Inc. 1996, San Diego, CA). While the coordinates for the bulk of the receptor was kept fixed, the program has the ability to define a radius of residues within 5 A distance from the LFM analog. As the modeling calculations progressed, the residues within the defined radius were allowed to shift and/or rotate to energetically favorable positions to accommodate the ligand. Calculations were carried out on an INDIGO2 computer (Silicon Graphics Inc., Mountain View, CA) using the CVFF force field in the Discover program and Monte Carlo search strategy in Affinity (Insightll, Molecular Simulations Inc. 1996, San Diego, CA). No solvation procedures were used. Conjugated gradient minimization was used to conserve CPU time, as the total number of movable atoms was greater than 200. Calculations approximating hydrophobic and hydrophilic interactions were used to determine the ten best docking positions of each LFM analog in the EGFR catalytic site. The various docked positions of each LFM analog was qualitatively evaluated using a score function in the Ludi module (Bohm, H. J. J. Comput. Aided. Mol. Des. 1992, 6, 593; Bohm, H. J. J. Comput. Aided Mol. Des. 1994, 8, 243) of the program INSIGHTII (Insightll, Molecular Simulations Inc. 1996, San Diego, CA) which was then used to estimate a binding constant (K,) for each compound in order to rank their relative binding capabilities and predicted inhibition of EGFR. The calculated K, trends for the LFM analogs were compared with the trend of the experimentally determined tyrosine kinase inhibition as well as cytotoxicity IC 50 values for the compounds, in order to elucidate the structure-activity relationships (SAR) determining the potency of LFM analogs. The docked inhibitors were sandwiched between two regions of mostly hydrophobic residues. The region above the inhibitor consisted of residues Leu 694 , Val 702 , Lys 721 , and Ala 719 , whereas the region below included residues Leu 820 and Thr 830 . The positions of the critical residues in the active site of the EGFR and the docked positions of the LFM analogs are shown in Figures 2-4. Of the possible orientations of the LFM analogs in this binding pocket, the one shown in Figures 2 - 4 had the highest interaction score and was assumed by all 15 compounds. This indicates that the depicted docked positions represent an energetically favored binding mode. In this binding mode the compounds can maintain close contact with the hinge region on the edge of the inhibitor, residues Leu 694 and Val 702 above the inhibitor, and Leu 820 and Thr 830 below. In all cases the nitrile nitrogen of the ligand was involved in hydrogen bonding with the amide NH of Met 769 . In addition the αra-substituted OCF 3 group appeared to form close contacts between residues Thr 766 and Asp 831 . From the modeling studies, it was apparent that ortho substitutions on the phenyl ring of LFM analogs would prevent the molecule from having close contact with the hinge region of the receptor. The poor interaction is reflected by the higher K, values calculated for this group of compounds (LFM-A4 - A7; Table 2). The results indicated a trend that for a smaller substitution at the ortho position, there would be less disruption of close contacts with the receptor. As the substituted group got bulkier, the inhibitor would be pushed further away from the hinge region of the receptor, thereby weakening the contacts. The compound containing the smallest ort/.ø-substituted group, LFM-A7 (with o-F) had the best interaction score in this group with a calculated K of 69 μM. Docking analysis suggested that LFM- A7 had slightly increased contact with EGFR residues relative to the unsubstituted compound, LFM-Al 4, consistent with its lower calculated K, value. The modeling also revealed that meta substituents, except for m-F, would likely be sandwiched between residues Thr 766 and Asp 831 . The m-F group of LFM- Al 1 was predicted to be located on the opposite side of the molecule relative to the metβ-substituted groups of the other 3 compounds, and docking showed that the m-F group of LFM-Al 1 would be located near Asn 818 , instead of the usual binding mode where the meta group is located between Thr 766 and Asp 831 , opposite Asn 81 s . The calculated K, values for compounds with m-F, rn-Cl and m-Br substitutions were greater than 100 μM (Table 2). The results shown in Table 2 indicate that LFM-A8, which has a m-CF 3 substitution, is the best fitting compound in this group with a calculated K, of 22 μM. While maintaining the close contact with the hinge region, the m-CF 3 substituent of LFM-A8 would extend further into a leucine-rich pocket of the protein (beyond Thr 766 and Asp 831 ) and gain more hydrophobic contact. Consequently, LFM-A8 was predicted to bind to the catalytic site of the EGFR kinase domain better than the other metα-substituted LFM analogs. The modeling also revealed that the LFM analogs with jαra substitutions would have the greatest potential for inhibition of the EGFR, with/.-CF 3 and/>-OCF, 2?- being the most promising (calculated Ki ~ 8 - 13 μM), followed by / C1 and ?-Br, (calculated K ~ 30 - 40 μM), and -F (calculated K >100 μM) (Table 2). The best docked positions of the five para substituted compounds showed a binding pattern similar to that of the meta substituted compounds except for a slight shift of the phenyl rings toward Thr 766 (Figure 3). The para substituted compounds would maintain a close contact with the hinge region of the EGFR kinase domain and be stabilized by additional contact area between the para substituents and the residues deep inside the binding site. The CF 3 and OCF 3 substituents in the para position would extend toward the deepest corner of the binding site which would result in improved molecular contact. For compound LFM-A3, the C-F bond length (1.3 A) is shorter than the C-Cl and C-Br bond lengths in LFM-A2 and LFM-Al (1.8 - 1.9 A) and thus the ?-F group would not approach Lys 721 as closely. This weaker contact may contribute to the poor K, values for LFM-A3. The para substituted compounds appeared to approach Asp 831 of EGFR more closely compared to ortho or meta substituted compounds. The result of this closer approach may be some steric strain with Asp 831 which may force the residue to rotate away from the inhibitor as was observed in docking results. This action actually disrupts a hydrogen bond between Asp 831 and Asn 830 which could cause a slight destabilization of the protein conformation in this region. This event is more likely to occur when larger 7_-αrα-substituted groups are involved, such as ϊhepara- r of LFM-Al and para-C\ of LFM-A2. The calculations did not incorporate the energetic effects of this speculated protein destabilization. Therefore, the true K, values for compounds containing a larger r -substituted group, such as LFM-Al and LFM-A2, may be higher than the estimated K, values shown in Table 2. In these studies, the para substituted CF was more active than the m-CF 3 compound in terms of IC 50 inhibition values (IC 5 o=5.4μM vs>100μM, Table 1), which is consistent with the modeling observation that this compound maintains closer contacts with the hinge region than the m-CF 3 compound. As shown in Figure 3, the meta substitution is sterically less favorable for allowing a closer interaction with the hinge region of EGF-R relative to para substituted LFM compounds, which may contribute to a loss of hydrophobic contact in this region of the binding site. This loss of hydrophobic contact would be reflected in a lower calculated K, value based on docking studies. The superimposed docked positions of the three CF 3 substituted compounds (ortho, meta, and para) are shown in Figure 4. The bulky ortho substitution would prevent the ligands from having close contact with the catalytic site and make it unlikely for this compound to show significant inhibitory activity. The para substituted compound, on the other hand, can maintain good contact with the hinge region of the receptor and would likely be an effective inhibitor by our calculations. The inhibition values for meta substituted compounds were predicted to fall between those of the ortho and para substituted compounds. The calculated K, values are consistent with their final docked positions which show that the m-CF 3 compound is located in-between the para and ortho compounds. Docking studies also showed that the ring substituents of most of the active compounds were predicted to be positioned between residues Thr 766 and Asp 831 of the EGFR. The modeling studies suggested that LFM-Al 2 would exhibit potent EGFR- inhibitory activity. In order to test this hypothesis and validate the predictive value of the described EGFR kinase domain homology model, LFM-Al 2, LFM, and 13 other LFM analogs listed in Table 2 were prepared. The three dimensional structures of 10 of these compounds were determined by single crystal X-ray diffraction . The crystal data, experimental parameters and refinement statistics are summarized in Table 1. All structures, except LFM-A2 (p -Cl), were found to be essentially planar in conformation, and all bond lengths and angles were in the expected range. For LFM-A2 the dihedral angle between the aromatic ring and side chain was close to 45°. In all crystal structures except LFM-Al 1 (m-F), the ortho or meta substituents were found to reside on the same side of the molecule as the nitrile group. In LFM-Al 1, the phenyl ring is rotated so that the meta-F and nitrile groups are on opposite sides of the molecule. The molecular coordinates of LFM and LFM analogs which were energy-minimized and docked into the EGFR binding site in modeling studies adopted a conformation similar to that of their crystal structures. The effects of a compound on EGFR tyrosine kinase and survival of human breast cancer cells can be determined using pharmacological models which are well known to the art, or using the biological tests described below. Example 4 Chemistry and Synthesis All chemicals were purchased from Aldrich (Milwaukee, WI) and were used without further purification. Except where noted, each reaction vessel was secured with a rubber septum, and the reaction was performed under nitrogen atmosphere. 'H spectra were obtained on a Varian Mercury 300 instrument spectrometer (Palo Alto, CA) at ambient temperature in the solvent specified. Melting points were determined using a Fisher- Johns melting point apparatus and are uncorrected. FT-IR spectra were recorded on a Nicolet Protege 460 spectrometer (Madison, WI). GC/MS spectra were obtained on a HP 6890 GC System (Palo Alto, CA) equipped with a HP 5973 Mass Selective Detector. Scheme 1 shows the general synthetic scheme for the preparations of LFM, and LFM-Al - LFM-A14 (Kuo, E. A., et al. J. Med. Chem. 1996, 39, 4608; Sjogren, E. R., et al. J. Med. Chem. 1991, 34, 3295). Cyanoacetic acid 1 was coupled with the REQUISITE substituted-aniline 2 in the presence of diisopropylcarbodiimide (DIC) to form 3. Compound 3 was treated with NaH and acylated with acetyl chloride to afford the final products. Scheme 1 LFM, LFM-Al - LFM-Al 4 LFM X =para-CF 3 LFM-A5 X = ortho-Bτ LFM-A10 X = weto-Cl LFM-Al X = para-Bτ LFM-A6 X = ottho-C\ LFM-Al 1 X = meta-F LFM-A2 X = /.__ra-Cl LFM-A7 X = oι tho-¥ LFM-A12 X = pαra-OCF j LFM-A3 X = para-F LFM-A8 X = meta-CF } LFM-A13 X = 2-Bι, 5-Br LFM-A4 X = ort/.o-CF 3 LFM-A9 X = eto-Br LFM-Al 4 X = H General Synthetic Procedures: The general synthetic procedures are described in the literature (Kuo, E A , et al J Med Chem 1996, 39, 4608, Sjogren, E R , et al J Med Chem 1991, 34, 3295) In general, 1,3-diisopropylcarbodnmide (1 75 g, 13 9 mmol) was added to a solution of cyanoacetic acid 1 (1 70 g, 20 0 mmol) and the requisite substituted- anilme 2 (12 6 mmol) in tetrahydrofuran (25 mL) at 0°C The mixture was stirred for 12 hours at room temperature The urea precipitate (reaction side product) was removed by filtration and the filtrate was partitioned between ethyl acetate and 0 5 N HCl The organic layer was sequentially washed with brine twice, dried over anhydrous Na ; ,SO 4 and concentrated by rotary-evaporation The solid product was recrystalhzed from ethyl alcohol to give pure 3 Sodium hydride (0 93 g, 60% m mineral oil, 23 2 mmol) was added slowly to the solution of 3 (12 0 m mol) in tetrahydrofuran (40 mL) at 0°C After stirring for 30 minutes at 0°C, the requsite acid chloride R,COCl (1 04g, 13 2 mmol) was added to the reaction mixture The reaction was continued for another hour at room temperature and then was quenched by the addition of acetic acid (2 mL) The mixture was poured into ice water (100 mL) containing 2 5 mL of hydiochloπc acid to precipitate the crude product, which was collected by filtration and washed with water The final pure product was obtained by recrystalhzation Physical data of specific compounds: α-Cyano-/3-hydroxy- ^methyl-N-[4-(trifluoromethyl)phenyl]- propenamide (LFM). mp: 230 - 233°C; IR (KBr): 3303, 2218, 1600 and 1555 cm " '; Η ΝMR (DMSO-J 6 ): δ 11.01 (s, IH, ΝH), 7.75 (d, J= 8.4 Hz, 2H, ArH), 7.64 (d, J= 8.4 Hz, 2H, ArH), 2.22 (s, 3H, CH 3 ); GC/MS m/z 270 (M + ), 161, 142, 111. α-Cyano- ^hydroxy-/3-methyl-N-(4-bromophenyl)propenamide (LFM- Al). mp: 213 - 214°C; IR (KBr): 3288, 2228, 1615, 1555 cm "1 ; Η ΝMR (DMSO- d 6 ): δ 10.51 (s, IH, ΝH), 7.49 (s, 4H, ArH), 2.25 (s, 3H, CH 3 ); MS (El) m/z 282(M + +z), 280 (M + ), 173, 171. α-Cyano- -?-hydroxy- ?-methyl-N-(4-chIorophenyl)propenamide (LFM- A2). mp: 209 - 211°C; IR (KBr): 3298, 2223, 1598 and 1552 cm 1 ; 'H ΝMR (DMSO-J 6 ): δ 10.48 (s, IH, ΝH), 7.54 (d, J= 8.7 Hz, 2H, ArH), 7.45 (s br, IH, OH), 7.36 (d, J= 8.7 Hz, 2H, ArH), 2.25 (s, 3H, CH 3 ); MS (CI) m/z 236 ( ), 121, 127. α-Cyano-/3-hydroxy-/-J-methyl-N-(4-fluorophenyl)propenamide (LFM- A3). mp: 165 - 166°C; IR (KBr): 3298, 2218, 1610 and 1560 cm 1 ; 'H ΝMR (DMSO-J 6 ): δ 10.33 (s, IH, ΝH), 7.80 (s br, IH, OH), 7.53 (m, 2H, ArH), 7.16 (m, 2H, ArH), 2.26 (s, 3H, CH 3 ); GC/MS m/z 220 (M + ), 111. α-Cyano-/3-hydroxy- ?-methyl-N-[2-(trifluoromethyl)phenyl]- propenamide (LFM-A4). mp: 61 - 63°C; IR (KBr): 3435, 2209, 1619, 1952 and 1548 cm '1 ; Η ΝMR (DMSO-J 6 ): δ 10.99 (s, IH, ΝH), 8.03 (d, J= 7.5 Hz, IH, ArH), 7.67 (d, J= 7.5 Hz, IH, ArH), 7.60 (dd, J= 7.5, 7.5 Hz, IH, ArH), 7.29 (dd, J= 7.5, 7.5 Hz, IH, ArH) 5.71 (s br, IH, OH), 2.20 (s, 3H, CH 3 ); GC/MS m/z 270 (M + ), 161, 141, 114. «-Cyano- ^hydroxy-/2-methyl-N-(2-bromophenyl)propenamide (LFM- A5). mp: 98 - 100°C; IR (KBr): 3351, 2214, 1609, 1585 and 1536 cm 1 ; Η ΝMR (DMSO-J 6 ): δ 10.76 (s, IH, ΝH), 8.06 (dd, J= 8.1, 1.5 Hz, IH, ArH), 7.62 (dd, J = 8.1, 1.5 Hz, IH, ArH), 7.33 (m, IH, ArH), 7.03 (m, IH, ArH), 6.60 (s br, IH, OH), 2.22 (s, 3H, CH 3 ); ); MS (El) m/z 282(M ^ +z), 280 (JVT), 173, 171. „ 36 α-Cyano- ^hydroxy- ^methyl-N-(2-chIorophenyl)propenamide (LFM- A6). mp: 93 - 94°C; IR (KBr): 3372, 2208, 1644, 1621 and 1587 cm 4 ; 'H ΝMR (DMSO-J 6 ): δ 10.96 (s, IH, ΝH), 8.16 (d, J= 8.1 Hz, IH, ArH), 7.46 (dd, J- 7.5, 1.5 Hz, IH, ArH), 7.29 (m, IH, ArH), 7.08 (m, IH, ArH), 2.22 (s, 3H, CH 3 ); MS (CI) m/z 236 (M + ), 29, 127. α-Cyano-/^hydroxy-/^methyl-N-(2-fluorophenyl)propenamide (LFM- A7). mp: 118 - 119°C; IR (KBr): 3409, 2212, 1613, 1591 and 1532 cm 1 ; 'H ΝMR (DMSO-J 6 ): δ 10.70 (s, IH, ΝH), 7.91 (m, IH, ArH), 7.23 (M, IH, ArH), 7.13 (m, 2H, ArH), 7.10 (s br, IH, OH), 2.22 (s, 3H, CH 3 ); GL/MS m/z 220 (M τ ), 111. α-Cyano-/-^hydroxy- ^methyI-N-[3-(trifluoromethyl)phenyI]- propenamide (LFM-A8). mp: 182 - 184°C; IR (KBr): 3303, 2221, 1619 and 1572 cm 1 ; 'H ΝMR (DMSO-J 6 ): δ 10.79 (s, IH, ΝH), 8.05 (s br, IH, OH) 8.04 (s, IH, ArH), 7.75 (d, J= 8.1 Hz, IH, ArH), 7.53 (dd, J= 8.1, 7.5 Hz, IH, ArH), 7.42 (d, J = 7.5 Hz, IH, ArH), 2.24 (s, 3H, CH 3 ); GL/MS m/z 270 (M + ), 161. α-Cyano- ?-hydroxy- ?-methyI-N-(3-bromophenyl)propenamide (LFM- A9). mp: 184 - 185°C; IR (KBr): 3303, 2228, 1610, 1595 and 1550 cm 1 ; 'H ΝMR (DMSO- 6 ): δ 10.56 (s, IH, ΝH), 7.89 (m, IH, ArH), 7.47 (m, IH, ArH), 7.28 (m, 2H, ArH), 6.37 (s br, IH, OH), 2.26 (s, 3H, CH 3 ); MS (El) m/z 282 (M τ + H, 81 Br), 280 (M + +H, 79 Br), 171, 173. - Cyano-/3-hydroxy- ?-methyl-N-(3-chlorophenyI)propenamide (LFM- A10). mp: 184 - 187°C; IR (KBr): 3293, 2221, 1610, 1595 and 1557 cm "1 ; Η ΝMR (DMSO-J 6 ): δ 10.61 (s, IH, ΝH), 7.76 (m, IH, ArH), 7.42 (m, IH, ArH), 7.33 (M, IH, ArH), 7.16 (m, IH, ArH), 6.84 (S br, IH, OH), 2.25 (s, 3H, CH 3 ); MS (CI) m/z 236 (M + ). α-Cyano- ^hydroxy- ^methyl-N-(3-fluorophenyI)propenamide (LFM- All). mp: 136 - 138°C; IR (KBr): 3297, 2221, 1613, 1597 and 1567 cm 1 ; Η ΝMR (DMSO-J 6 ): δ 10.54 (s, IH, ΝH), 7.54 (m, IH, ArH), 7.33 (m, 2H, ArH), 6.93 (m, IH, ArH), 2.27 (s, 3H, CH 3 ); GL/MS m/z 220 (-VT), 111. α-Cyano- ^hydroxy- ^methyl-N-[4-(trifluoromethoxy)phenyI]- propenamide (LFM-A12). mp: 182 - 183°C; IR (KBr): 3308, 2213, 1625 and 1580 cm 1 ; 'H NMR (DMSO-J 6 ): δ 10.57 (s, IH, NH), 7.90 (s br, IH, OH), 7.64 (d, J = 8.7 Hz, 2H, ArH), 7.32 (d, J= 8.7 Hz, 2H, ArH), 2.25 (s, 3H, CH 3 ); GL/MS m/z 286 (W), 111, 108. <2-Cyano- -?-hydroxy- _?-methyl-N-(2,5-dibromophenyl)propenamide (LFM-A13). mp: 148 - 150°C; IR (KBr): 3353, 2211, 1648 and 1590 cm "1 ; Η ΝMR (DMSO-J 6 ): δ 11.41 (s, IH, ΝH), 8.57 (d, J= 2.4 Hz, IH, ArH), 7.55 (d, J = 8.7 Hz, IH, ArH), 7.14 (dd, J= 8.7, 2.4 Hz, IH, ArH), 7.10 (s br, IH, OH), 2.17 (s, 3H, CH 3 ); MS (El) m/z 362 (M + + 4), 360 (WT + 2), 358 (M + ), 253, 251, 249, 150. α-Cyano- ^hydroxy- ?-methyl-N-(phenyl)propenamide (LFM-A14). mp: 134 - 135°C; IR (KBr): 3281, 2214, 1605, 1579 and 1554 cm 4 ; 'H ΝMR (DMSO- d 6 ): δ 10.33 (s, IH, ΝH), 7.51 (d, J= 7.5 Hz, 2H, ArH), 7.40 (s br, IH, OH), 7.31 (dd, J = 7.5, 7.5 Hz, 2H, ArH), 7.11 (m, IH, ArH), 2.26 (s, 3H, CH 3 ); GL/MS m/z 202 (M + ), 93. Example 5 Biological Tests Immunoprecipitation of Recombinant Proteins from Insect Cells: Sf21 cells were infected with a baculovirus expression vector for BTK, JAKl, or JAK3, as previously reported (Vassilev, A., et al. J. Biol. Chem. 1998, 274, 1646-1656; Goodman, P. A., et al. J. Biol. Chem. 1998, 273, 17742). Cells were harvested and lysed (lOmM Tris pH 7.6, lOOmM ΝaCl, 1% Νonidet P-40, 10% glycerol, 50mM ΝaF, lOOmM Νa 3 VO 4 , 50mg/ml phenylmethylsulfonyl fluoride, 1 Omg/ml aprotonin, 1 Omg/ml leupeptin) and the kinases were immunoprecipitated from the lysates, as reported (Vassilev, A., et al. J. Biol. Chem. 1998, 274, 1646- 1656). Antibodies used for immunoprecipitations from insect cells are as follows: Polyclonal rabbit anti-BTK serum (Mahajan, S., et al. Mol. Cell. Biol. 1995, 15, 5304), polyclonal rabbit anti-JAKl (HR-785), cat# sc-277, rabbit polyclonal IgG affinity purified, 0.1 mg/ml, Santa Cruz Biotechnology; and polyclonal rabbit anti- JAK3 (C-21, cat # sc-513, rabbit polyclonal IgG affinity purified, 0.2 mg/ml, Santa Cruz Biotechnology). Kinase assays were performed following a 1 hour exposure of the immunoprecipitated tyrosine kinases to the test compounds, as described in detail elsewhere (Mahajan, S., et al. Mol. Cell. Biol 1995, 15, 5304; Uckun, F. M., et al. Science 1996, 22, 1096). The immunoprecipitates were subjected to Western blot analysis as previously described (Vassilev, A., et al. J. Biol. Chem. 1998, in press). Cell lines, Reagents, and Biochemical Assays: MDA-MB-231 (ATCC HTB-26) and MDA-MB-361 (ATCC HTB- 27) are EGFR positive human breast cancer cell lines (Uckun, F. M., et al. Clin. Can. Res. 1998, 4, 901). These cell lines were maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum. For subculturing, medium was removed from the flasks containing a confluent layer of cells, and fresh 0.25% trypsin was added for 1-2 min. Trypsin was removed and cultures incubated for 5- 10 min. at 37°C until cells detached. Fresh medium was then added, and cells were aspirated and dispensed into new flasks. COS-7 simian kidney cell line and HepG2 human hepatoma cell line were obtained from ATCC. Antibodies directed against BTK, JAKl, JAK3, and HCK have been described previously (Vassilev, A., et al. J. Biol. Chem. 1998, in press ; Goodman, P. A., et al. J Biol. Chem. 1998, 273, 17742; Mahajan, S., et al. Mol. Cell. Biol. 1995, 15, 5304; Uckun, F. M., et al. Science 1996, 22, 1096). Polyclonal antibodies to BTK were generated by immunization of rabbits with glutathione S-transferase (GST) fusion proteins (Pharmacia Biotech Inc.) containing the first 150 amino acids of BTK. The monoclonal anti-Fas antibody (F22120) was obtained from the Transduction Laboratories, Inc. (Lexington, KY). Immunoprecipitations, immune- complex protein kinase assays, and immunoblotting using the ECL chemiluminescence detection system (Amersham Life Sciences) were conducted as described previously (Vassilev, A., et al. J. Biol. Chem. 1998, in press; Goodman, P. A., et al. J. Biol. Chem. 1998, 273, 17742; Mahajan, S., et al. Mol. Cell Biol. 1995, 15, 5304; Uckun, F. M., et al. Science 1996, 22, 1096). Horse radish peroxidase-conjugated sheep anti-mouse, donkey anti-rabbit secondary antibodies and ECL reagents were purchased from Amersham (Oakbrook, IL). For insulin receptor kinase (IRK) assays, HepG2 human hepatoma cells grown to approximately 80%o confluency were washed once with serum-free DMEM and starved for 3 hour at 37° in a CO 2 incubator. Subsequently, cells were stimulated with insulin (Eli Lilly, cat# CP-410;10 units/ ml/10 xlO 6 cells) for 10 minutes at room temperature. Following this IRK activation step, cells were washed once with serum free medium, lysed in NP-40 buffer and IRK was immunoprecipitated from the lysates with an anti-IRb antibody (Santa Cruz, Cat.# sc-711, polyclonal IgG). Prior to performing the immunecomplex kinase assays, the beads were equilibrated with the kinase buffer (30mM Hepes pH 7.4, 30mM NaCl, 8mM MgC12, 4mM MnCl 2 ). For HCK kinase assays, we used HC .-transfected COS-7 cells. The cloning and expression of ΗCK in COS-7 cells has been described previously (Saouaf, S. J., et al. J. Biol Chem. 1995, 270, 27072). The pSV7c-ΗCK plasmid was transfected into 2 xlO 6 COS-7 cells using Lipofectamine (GIBCO/BRL), and the cells were harvested 48 hours later. The cells were lysed in NP-40 buffer and HCK was immunoprecipitated from the whole cell lysates with an anti-HCK antibody. Immune-Complex Kinase Assays and Anti-Phosphotyrosine Immunoblotting: For EGFR immune complex kinase assays, 24-hours after treatment with leflunomide analogs, MDA-MB-231 breast cancer cells were stimulated with 20 ng/mL EGF for 5 minutes, lysed in 1% Nonidet-P-40 buffer, and cell lysates were immunoprecipitated with an anti-EGFR antibody reactive with the sequence Ala 33 '- Asp 364 of the human EGFR (Upstate Biotechnology Inc. [UBI] Catalog # 05-104) (Uckun, F. M., et al. Clin. Can. Res. 1998, 4, 901). EGFR immune complexes were examined for tyrosine phosphorylation by Western blot analysis, as described by Uckun, F. M., et al. Clin. Can. Res. 1998, 4, 901. All anti-phosphotyrosine Western blots were subjected to densitometric scanning using the automated AMBIS system (Automated Microbiology System, Inc., San Diego, CA) and for each time point a % inhibition value was determined by comparing the density ratios of the tyrosine Λ n 40 phosphorylated EGFR protein bands to those of the baseline sample and using the formula: % Inhibition = 100 - 100 x [Density of tyrosine phosphorylated EGFR band] test sample / [Density of tyrosine phosphorylated EGFR band] baseline contro , sample . The IC 50 values were determined using an Inplot program (Graphpad Software, Inc., San Diego, CA). In other experiments, MDA-MB-231 cells were stimulated with 10 ng/ml EGF prior to immunoprecipitation of the EGFR. EGFR immune complexes were incubated for 1 hour at room temperature with various LFM analogs and tyrosine kinase assays were performed in the presence of [γ- 32 P]-ATP, as previously described (Uckun, F. M., et al. Clin. Can. Res. 1998, 4, 901 ; Naria, R. K., et al. Clin. Can. Res 1998, 1405; Uckun, F. M., et al. Science 1996, 22, 1096; Uckun, F. M., et al. Science 1995, 267, 886). The kinase assay gels were analyzed both by autoradiography and using the BioRad Storage Phosphor Imager System (BioRad, Hercules, CA) for quantitative scanning. In Vitro Treatment of Cells with LFM Compounds: In order to determine the cytotoxic activity of LFM and its analogs against breast cancer cells, cells in alpha-MEM supplemented with 10%(v/v) fetal calf serum were treated with various concentrations of the compounds for 24 hours at 37°C, washed twice in alpha-MEM, and then used in either MTT assays or in vitro invasion assays, as described hereinafter. Cytotoxicity Assay: The cytotoxicity of various compounds against human breast cancer cell lines was analyzed using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay (Boehringer Mannheim Corp., Indianapolis, IN). Uckun F.M., et al. Clin. Cancer Res. 1998, 4, 901-912. Exponentially growing breast cancer cells were seeded into a 96-well plate at a density of 2.5 x 10 4 cells/well and incubated for 36 hours at 37°C prior to drug exposure. On the day of treatment, culture medium was carefully aspirated from the wells and replaced with fresh medium containing the LFM analogs at concentrations ranging from 2 to 250 μM. Triplicate wells were used for each treatment. The cells were incubated with the various compounds for 36 hours at 37°C in a humidified 5% CO 2 atmosphere. To each well, 10 μl of MTT (0.5 mg/ml final concentration) was added and the plates were incubated at 37°C for 4 hours to allow MTT to form formazan crystals by reacting with metabolically active cells. The formazan crystals were solubilized overnight at 37°C in a solution containing 10% SDS in 0.01 M HCl. The absorbence of each well was measured in a microplate reader (Labsystems) at 540 nm and a reference wavelength of 690 nm. To translate the absorbance A 540 values into the number of live cells in each well, the A 540 values were compared to those on standard A 540 - versus - cell number curves generated for each cell line. The percent survival was calculated using the formula: % survival = Live cell number[test] / Live cell number [control] x 100. The IC 50 values were calculated by non-linear regression analysis using an Graphpad Prism software version 2.0 (Graphpad Software, Inc., San Diego, CA). Confocal Laser Scanning Microscopy: Immunofluorescence was used to examine the morphologic features of breast cancer cells treated with LFM and its analogs. Before the experiment, cells were trypsinized from rapidly growing tissue culture flasks and seeded onto sterile 22 mm 2 coverslips in 6-well culture plates. Cells on coverslips were returned to the incubator for 24 hours prior to treatment. The following day, drugs were added from a stock solution made in DMSO to a final concentration of 100 μ M. Final DMSO concentration was 0.1% in both test samples and controls. Cells were returned to a 37° C incubator for 24 hours before further processing. At 24 hours, coverslips were fixed in -20° C methanol for 15 minutes followed by a 15 minute incubation in phosphate buffered saline + 0.1% Triton X-IOO(PBS-Tx). Coverslips were next incubated with a monoclonal antibody against α-tubulin (Sigma Chemical Co, St. Louis, MO) at a dilution of 1 : 1000 for 40 minutes in a humidified chamber at 37° C. Coverslips were washed for 15 minutes in PBS-Tx followed by a 40 min incubation with a goat anti-mouse IgG antibody conjugated to FITC (Amersham Corp., Arlington Heights, IL). The Λ n 42 coverslips were again rinsed in PBS-Tx and incubated with 5 μM TOTO-3 (Molecular Probes, Eugene OR) for 20 minutes to label the nuclear DNA. Coverslips were immediately inverted onto slides in Vectashield (Vector Labs, Burlingame) to prevent photobleaching, sealed with nail varnish and stored at 4°C. Slides were examined using a Bio-Rad MRC-1024 Laser Scanning Confocal Microscope mounted on a Nikon Eclipse E800 upright microscope with high numerical aperture objectives. Digital data was processed using Lasersharp (BioRad, Hercules CA) and Adobe Photoshop softwares (Adobe Systems, Mountain View, CA) and printed on a Fuji Pictography thermal transfer printer (Fuji, Elmsford, NY). Apoptosis Assays: Loose packing of membrane phospholipid head groups and cell shrinkage precede DNA fragmentation in apoptotic cells, thereby providing MC540 binding as an early marker for apoptosis (Uckun F.M., et al. Clin. Cancer Res. 1998, 4, 901-912; and Kuo, E.A. J. Med. Chem. 1996, 39, 4608-4621). Plasma membrane permeability to propidium iodide (PI, Sigma) develops at a later stage of apoptosis (Uckun F.M., et al. Clin. Cancer Res. 1998, 4, 901-912; and Kuo, E.A. J. Med. Chem. 1996, 39, 4608-4621). MC540 binding and PI permeability were simultaneously measured in breast cancer cells 24 hours after exposure to leflunomide analogs, as described (Uckun F.M., et al. Clin. Cancer Res. 1998, 4, 901-912. Stock solutions ofMC540 and PI, each at 1 mg/mL, were passed through a 0.22 μm filter and stored at 4°C in the dark. Shortly before analysis, suspensions containing lxl 0^ cells were suspended in 5 μg/mL MC540 and 10 μg/mL PI and kept in the dark at 4°C. Whole cells were analyzed with a FACS Calibur or FACS Vantage flow cytometer (Becton Dickinson, San Jose, Ca). All analyses were done using 488 nm excitation from an argon laser. MC540 and PI emissions were split with a 600 nm short pass dichroic mirror and a 575 nm band pass filter was placed in front of one photomultiplier tube to measure MC540 emission and a 635 nm band pass filter was used for PI emission. Clonogenic Assays: After treatment with LFM analogs, cells were resuspended in clonogenic medium consisting of alpha-MEM supplemented with 0.9% methylcellulose, 30% fetal bovine serum, and 50 μM 2-mercaptoethanol. Cells were plated in duplicate Petri dishes at 100,000 cells/mL/dish and cultured in a humidified 5% CO2 incubator for 7 days. Cancer cell colonies were enumerated on a grid using an inverted phase microscope of high optical resolution (Uckun F.M., et al. Clin. Cancer Res. 1998, 4, 901-912; and Kuo). Results were expressed as % inhibition of clonogenic cells at a particular concentration of the test agent using the formula: % Inhibition = (1 - Mean # of colonies [Test] / Mean # of colonies [Control]) x 100. Furthermore, the dose survival curves were constructed using the percent control survival (= Mean # of colonies[Test] / Mean # of colonies [Control] x 100) results for each drug concentration as the data points and the IC50 values were calculated. The IC50 values were determined using an Prism Version II Inplot program (Graphpad Software, Inc., San Diego, CA). Transfllter Cell Invasion Assays: The in vitro invasiveness of MDA-MB-231 human breast cancer cells was assayed using a previously published method, which employs Matrigel-coated Costar 24-well transwell cell culture chambers ("Boyden chambers") with 8.0 μm pore polycarbonate filter inserts which have been demonstrated to permit the migration of human cancer cells (Yoshida, D., et al. Neurosurgery 1996, 39, 360). The chamber filters were coated with 50 μg/ml of Matrigel matrix, incubated overnight at room temperature under a laminar flow hood and stored at 4°C. On the day of the experiment, the coated inserts were rehydrated with 0.5 ml serum-free DMEM containing 0.1% bovine serum albumin for 1-2 hours. To study the effects of LFM and LFM-A12 on invasiveness of MDA-MB-231 cells, exponentially growing cells were incubated with each compound at various concentrations ranging from 12.5 μM to 100 μM in 0.1% DMSO overnight. The cells were trypsinized, washed twice with serum-free DMEM containing BSA, counted and resuspended at 1 x 10 5 cells/ml. A 0.5 ml cell suspension containing 5 x 10 4 cells in a serum-free DMEM containing LFM, LFM-Al 2, or vehicle was added to the Matrigel-coated and rehydrated filter inserts. Next, 750 μl of NIH fibroblast conditioned medium was placed as a chemoattractant in 24-well plates and the inserts were placed in wells and incubated at 37°C for 48 hours. After the incubation period, the filter inserts were removed, the medium was decanted off and the cells on the top side of the filter that did not migrate were scraped off with a cotton-tipped applicator. The invasive cells that migrated to the lower side of the filter were fixed, stained with Hema-3 solutions and counted under microscope. No cells were detected at the bottom of the Boyden chambers. Therefore, the number of cells on the lower side of the filters accounted for all cells that had migrated through the filter. Five (5) to ten (10) random fields per filter were counted to determine the mean (±SE) values for the invasive fraction. The invasive fractions of cells treated with LFM or LFM-Al 2 were compared to those of vehicle (0.1% DMSO in PBS) treated control cells and the percent inhibition of invasiveness was determined using the following formula: % Inhibition = lOOx (1 - Invasive Fraction of Drug-Treated Cells/Invasive Fraction of Control Cells). RESULTS Specific Inhibition of the EGFR Tyrosine Kinase by LFM-A12: The effects of LFM analogs on the enzymatic activity of the EGFR kinase in cell-free immune complex kinase assays were examined. As shown in Figure 5 A, a one hour incubation with LFM or LFM-Al 2 inhibited the EGFR tyrosine kinase in a dose-dependent fashion in anti-EGFR immunoprecipitates from lysates of MDA-MB-231 human breast cancer cells. The IC 50 values for EGFR inhibition were 5.4 μM for LFM and 1.7 μM for LFM-A12 (Figure 5A). In contrast, the IC 50 values for all other compounds were >100μM, except for LFM-A7 which was 74.5 μM. The effects of LFM and LFM analogs on the enzymatic activity of the EGFR tyrosine kinase in breast cancer cells were also examined. After a 24 hour exposure to LFM or LFM analogs, MDA-MB-231 cells were stimulated with EGF for 10 minutes, and EGFR immune complexes from whole cell lysates were subjected to Western blot analysis with a polyclonal anti-phosphotyrosine antibody to measure the autophosphorylation of the EGFR. Treatment of MDA-MB-231 cells with LFM-Al 2 and, albeit to a lesser extent, with LFM resulted in decreased tyrosine phosphorylation of the EGFR after EGF stimulation. In contrast, none of the other LFM analogs tested side-by-side were able to inhibit the EGFR kinase in cell-free (Table 2) or cellular ( not shown) EGFR kinase inhibition assays. Taken together, these experimental results are consistent with the general trend for most of the calculated K ; values shown in Table 2, thereby confirming the predictive value of the constructed homology model of the EGFR kinase domain. In MTT assays (Uckun, F. M., et al. Clin. Can. Res. 1998, 4, 901; Naria, R. K., et al. Clin. Can. Res 1998, 1405), LFM-Al 2 exhibited significant cytotoxicity against the MDA-MB-361 human breast cancer cell line with mean IC 50 value of 26.3 μM. By comparison, LFM was significantly less active against these breast cancer cells. The IC 50 value for the LFM composite dose survival curves was 190.5 μM for MDA-MB-361 cells. The inhibitory effects of LFM and LFM-Al 2 on the EGFR tyrosine kinase were specific in that they did not affect the enzymatic activity of other protein tyrosine kinases, including receptor family tyrosine kinase IRK (Figure 7B), Src family tyrosine kinase HCK (Figure 7C), Janus kinases JAKl and JAK3 (Figures 7D-E), and Tec family tyrosine kinase BTK (Figure 7F), at concentrations as high as 350 μM (Table 3). Table 3. LFM-A12 interaction scores, calculated K ; values and measured IC 50 values for the inhibition of several protein tyrosine kinases. ΗB = Number of hydrogen bonds between inhibitor and protein b Ludι K, calculated based on the empirical score function m Ludi program (Bohm, H. J J Comput Aided Mol Des 1992, 6, 593; and 36. Bohm, H. J. J Comput Aided Mol Des 1994, 8, 243). Cell-free tyrosine kinase inhibition assays were performed as described above and the IC50 values were calculated from the LFM-Al 2 concentration-kmase activity curves. Modeling studies were performed using the crystal structure coordinates of HCK (Sicheri et al., 1997, Nature, 385:602-9) and IRK (Hubbard, 1997, the EMBO Journal, 16:5572-5581) and constructed homology models for the kinase domains of JAKl, JAK3 ((Sudbeck et al., 1999, Clin. Can. Res. (in press)) and BTK (Mahajan et al., 1999, JBC, in press) to identify possible causes for the observed selectiveity of LFM-A12 for the EGFR tyrosine kinase. While most of the catalytic site residues of the EGFR kinase domain were conserved realtive to other PTKs, we noted a few specific variatoins. EGFR residues Leu 694 , Val 702 , Lys 721 , and Ala 719 are conserved in EGFR, HCK, FGFR and IRK. Residues Asn 818 and Asp 831 (opposite to hinge) are converved in EGFR, HCK, FGFR, IRK, BTK, JAKl and JAK3. Residues Cys 751 and Thr 830 are specific for EGFR but vary in BTK (Val, Ser), JAKl (Val, Gly), JAK3 (Val, Ala), IRK (Val, Gly), and HCK (Val, Ala). Residues Thr 766 and Leu 768 4? in the hinge region changes to Met and Leu in IRK, Met and Phe in JAKl, Met and Tyr in JAK3, and to Thr and Tyr in BTK. One region of the binding pocket contains Cys 773 in EGFR and is therefore considerably more hydrophobic than the corresponding residue of PDGFR (Asp), FGFR (Asn), JAKl (Ser), HCK (Ser), and IRK (Asp). LFM-Al 2 was docked into the kinase domains of IRK, HCK, JAK3, JAKl, BTK and EGFR. After energy minimization the compound maintained favorable close contacts with the hinge region of each kinase although the orientation of LFM- Al 2 in the catalytic site was different for BTK and HCK as shown in Figure 6 A. When bindign with EGFR the inhibitor appeared to be sanwiched between four residus, Leu 694 and Val 702 from above and Leu 820 and Thr 830 from below. The nitrile nitrogen of the ligand was involved in hydrogen bonding with the amide NH of Met 769 . In addition, the £>αrα -substituted OCF 3 group on the lead compound appeared to form close contacts between residues Thr 766 and Asp 831 at positions A and C, respectively, of EGFR (Figure 6B). Table 3 shows the interaction scores, estimated K, values, and measured IC 50 data for LFM-Al 2 with the different kinases. The data indicated that the selectivity of LFM-A12 for EGFR likely results from its molecular shape and from favorable interactions with unique EGFR residues that are not present in the kinase domains of the other PTKs. Likewise, unfavorable interactions with unique residues of the other PTKs that are not found in the EGFR kinase domain also contribute to this selectivity. These residue differences are illustrated in Figures 6A and 6B at positions A and B. Figure 6 A shows the backbone of the EGFR catalytic site, the residue differences between EGFR (white) and other kinases, and the docked position of LFM-Al 2 (multi-color) at this site in BTK (peach) which is also similar to the docked position in HCK (blue). Figure 6B shows the docked position of LFM-Al 2 (multi-color) in EGFR (white), which is also similar to the docked position in JAK3 (pink) and IRK (green). The dotted surface area in each figure represents the Connolly surface of the inhibitor LFM-A12. The aromatic residue in BTK (Tyr), HCK (Phe), JAKl (Phe), JAK3 (Tyr) (shown at position B in Figures 6 A and 6B, is not as favorable for interactions with the ?-OCF 3 group of LFM-Al 2. The corresponding residue in the EGFR kinase domain is leucine (shown in white at position B in Figures 6A and 6B), which would not cause such unfavorable interactions with LFM-A12. Also, for HCK there is a loss of hydrogen bonding interaction with LFM-A12. Furthermore, JAK3, IRK (shown in Figure 6B), and JAKl (not shown) contain a methionine residue (at position A in Figure 6B) which protrudes into the active site and could impair the close hydrophobic contact of LFM-Al 2 with the hinge region of the catalytic site. The longer methionine residue in JAK3 and IRK does not compliment the shape of LFM-A12 and may hinder its binding. As shown in Figure 6B, the corresponding residue in the EGFR kinase domain is threonine (white); its relatively shorter side chain enables LFM-Al 2 (multicolor) to have a more favorable hydrophobic contact with the hinge region which may result in tighter binding to the EGFR binding site. For EGFR, the most active compound (LFM-Al 2) appears to be located between the residues at positions A and C. Consequently, the estimated K, value for the EGFR (13 μM) was lower than the K, values for other PTKs which ranged from 27 μM for BTK to 1710 μM for JAK3 (Table 3). Confocal Imaging The effects of LFM-A12 treatment on MDA-MB-231 cells were examined by confocal laser scanning microscopy. Slides were examined using a Bio-Rad MRC-1024 Laser Scanning Confocal Microscope mounted on a Nikon Eclipse E800 upright microscope with high numerical aperture objectives. Digital data was processed using Lasersharp (Bio-Rad, Hercules CA) and Adobe Photoshop software (Adobe Systems, Mountain View, CA) and printed on a Pictography printer (Fuji, Elmsford, NY). Bohm, H.J. J. Comput. Aided Mol. Des., 1994, 8, 243-256. As shown in Figure 8A, vehicle (DMSO) treated control cells were round and large with many well organized microtubules (green fluorescence secondary to tubulin staining) in the cytoplasm. Nuclei (blue fluorescence secondary to TOTO-3 staining) were also round and homogenous. In contrast, MDA-MB-231 cells treated with 100 μM LFM-A12 for 24 hours were much smaller and had an abnormal shape with large cytoplasmic vacuoles (Figure 8B). The microtubules of LFM-Al 2 treated cells were fewer in number and they appeared less organized than those of DMSO treated controls. The nuclei (blue) of the LFM-A12 treated cells were also smaller and misshapen. Unlike LFM-Al 2, 100 μM LFM- A3 did not affect the morphology or microtubular organization of MDA-MB-231 cells (Figure 8C). Apoptosis Assays The morphologic features of LFM- A 12 treated MD A-MB -231 cells by immunocytohemistry (i.e., shrinkage, nuclear condensation, and abnormal microtubular organization) suggested that these cells might be undergoing apoptosis. Therefore, to determine whether LFM-Al 2 could trigger apoptosis in breast cancer cells, a quantitative flow cytometric apoptosis detection assay was performed. Loose packing of membrane phospholipid head groups and cell shrinkage precede DNA fragmentation in apoptotic cells, thereby providing MC540 binding as an early marker for apoptosis (Uckun, F.M. et al., Science, 1995, 267, 886-891). MC540 binding and propidium iodide (PI) permeability of MDA-MB- 231 breast cancer cells were simultaneously measured before and after a 24 hour treatment with 100 μM or 500 μM LFM-A12. Whole cells were analyzed with a FACStar Plus flow cytometer (Becton Dickinson, San Jose, CA). Whereas less than 5 % of MDA-MB-231 cells showed apoptotic changes after DMSO treatment, a significant portion of cells underwent apoptosis within 24 hours after LFM-A12 treatment (Apoptotic fraction [AF] with MC5407PL advanced stage apoptosis: 54% at 100 μM and 85% at 500 μM) (Figure 9). LFM, albeit to a lesser extent, also induced apoptosis in MDA-MB-231 cells. Clonogenic Assays The anti-cancer activity of LFM and LFM-Al 2 against MDA-MB- 361 and MDA-B-231 breast cancer cells was tested using in vitro clonogenic assays. As shown in Table 4, 24 hour treatment with LFM or LFM-Al 2 inhibited the clonogenic growth of MDA-MB-361 cells as well as MDA-MB-231 cells in a dose-dependent fashion. At 100 μM, our lead compound LFM-A12 killed 87.3 % of clonogenic MDA-MB-361 cells and >99% of clonogenic MDA-MB-231 cells. Table 4. In Vitro Anti-Tumor Activity of LFM and LFM-A12 Against Clonogenic Breast Cancer Cells Effects of LFM-A12 on MDA-MB-231 Breast Cancer Cell Invasion Through Matrigel Matrix: Matrigel matrix is made up of growth factors and several extracellular matrix (ECM) components, including collagens, laminin and proteoglycans. As shown in Figures 7A and 7B, MDA-MB-231 human breast cancer cells were highly invasive in Matrigel-coated Boyden chamber (CON). LFM-A12 inhibited the invasion of MDA-MB-231 cells through the Matrigel matrix in a dose-dependent fashion with an IC 50 value of 28.4 μM and it was more potent than LFM which had IC 50 value of 97.0 μM (Figures 7A and 7B). Example 6 Design of New Analogs Exploring the catalytic site of EGFR for the design of specific inhibitors The binding volume of the EGFR catalytic site is much larger than the volume occupied by the lead compound LFM-Al 2. Increasing the size of the ligand is postulated to increase the contact area between the receptor and ligand and hence enhance binding. Structural and chemical features of LFM analogs which aid binding to the EGFR catalytic site are described below and illustrated in Figures 10- 11. Table 5 shows the residue differences at the ATP binding site between the six PTK's : EGFR, Btk, Hck, Jakl, Jak3 and IR. Table 5. Residue differences between EGFR, Btk, Hck, Jakl, Jak3 and IR at the ATP binding pocket . T830, C751, T766, L764, L768, C773, R817 are some selected residues of the EGFR ATP binding site. Some or all of these residues are different from the ATP binding site of Btk, IR, Jakl , Jak3 and Hck. Amongst them, the residues Thr830 and Cys751 are specific for EGFR. These nonconserved residues can be utilized for the design of more potent and selective inhibitors of EGFR. In particular, targeting these residues for interaction with specific inhibitor moieties would impact the binding and stability of the inhibitor in the binding pocket, and enhance the specificity of the inhibitor for EGFR. Listed in Table 6 are some groups which are proposed to aid binding to the EGFR catalytic domain, for example by providing favorable groups for interaction with the EGFR kinase active site. Table 6. Substitutions on LFM analogs likely to increase the binding affinity for EGFR. Referring to Figure 10A, the lead compound LFM-Al 2 is shown bound to the EGFR-kinase domain in the predicted binding mode. A second possible bindign mode is shown in Figure 10B. Our modeling studies indicate that para substituted compounds maintain good contact with the hinge region fo the receptor and appear to be good inhibitors by our calculations. For the para substituted compounds, a second mode of binding may also be possibly where the molecule is roated 180° such that the aromatic ring is near residues P770, F771, G772, and the chain is new residues L764, T766, D831 (Figure 10B). In order to increase the affinity and specificity of the existing ligang (LFM- A12), more ineractions with the active site residues are desired. Substitutions at positions R, and R 6 -R 7 of formula II are expected to lead to increased binding affinity at the catalytic site of EGFR. This expectation is based on the observation that the catalytic site of the EGFR kinase domain is much larger (volumne of about 50θA 3 ) than the volue occupied by our most potent compounds. Increasing the size of the ligand, preferably to fill up to about 2/3 of the volume (about 40θA 3 ) is predicted to increase the contact area between the receptor and ligand and thus enhance binding. The designed compounds of formulae III-VI are expected to provide such increased contact with the receptor, have enhanced binding, and potent inhibitory activity. Novel compounds designed to fit and interact with specific contact points of the EGFR-TK binding pocket have the following structural formulae (III-VI): (III) where R 5 is H, NH 2 , CH 3 , OH, CF 3 , or halo. Preferably, R 5 is not H, and halo is Br or Cl. (IV) where R 5 is H, NH 2 , CH 3 , OH, CF 3 , or halo. Preferably, R, is not H, and halo is F or Cl. (V) where R 4 is NH-CH- or OCH 3 (VI) where R, is -CH 2 -CH 2 X and X is halo, preferably Cl or Br; or ^ is -CH 2 CF 3 ; or R, is: -N O These compounds are synthesized as detailed in the following schemes, according to their type of modifications. By using the proper substituted-aniline, the compounds shown as formulae III, IV, and V may be synthesized by the same general synthetic pathway used above for Example 4 (Scheme 1). Synthetically, the entry for synthesizing the compounds of formula VI is to use different acylating agent, acid chloride, in the last step of the synthetic pathway shown in Scheme 1. The following schemes illustrate the four general types of modifications, and general synthesis schemes for the desired substituted-analines for the four types. Scheme 3: Synthesis of the Desired Substituted -Anilines for Type I Modification (Compounds of Formula III) Scheme 3 illustrates a strategy for the synthesis of the desired substituted-analines for Type-I modification. All the starting materials used in Scheme 3 are commercially available. Scheme 4: Synthesis of the Desired Substituted- Analines for Type II Modification (Compounds of Formula IV) Scheme 4 is one approach to synthesizing all the needed substituted-analines for Type-II modification, e.g., the production of compounds of formula V. All the starting material used in Scheme 4 are commercially available. BrCH 2 CH 2 OCH 2 CH 2 Br/01T HNO. X = NH 2 ; CH 3 ; OH; CF 3 ; Cl; F | Scheme 5: Synthesis of the Desired Substituted- Analines for Type HI Modification (Compounds of Formula V) For type III modification, producing compounds of formula VI, the starting substituted-analine for synthesizing the compound of R 3 = -OCH 3 is commercially available. The starting material for synthesizing the compound of R 3 = -NH-CH3 is shown in Scheme 5. H 3 Scheme 6: The Desired Acid Chlorides for Type IV Modification (Compounds of Formula VI) For the type IV modification, to produce the compounds of formula VII, the desired acid chlorides, 4-morpholinecarbonyl chloride (4), mehtyl chloroforunate, (5), methyl chlorothioformate (6), trifluoroacetyl chloride (7), as shown in Scheme 6, are commercially available. C: * 5 & ' All publications, patents, and patent documents are incorporated by reference herein, as though individually incorporated by reference. The invention has been described with reference to various specific and preferred embodiments and techniques. However, it should be understood that many variations and modifications may be made while remaining within the spirit and scope of the invention.

Claims: WE CLAIM: 1. A compound of formula I: (I) where: R, is (C,-C 3 )alkyl, ( 3 -C 6 )cycloalkyl, phenyl, or NR a R b ; R 2 is hydroxy, (C,-C 6 )alkoxy, or (C,-C 6 )alkanoyloxy; R 3 is cyano, or (C,-C 3 )alkanoyl; R 4 is hydrogen, or (C,-C 3 )alkyl; R 5 is aryl, or heteroaryl; R, and R b are each independently hydrogen, or (C,-C 3 )alkyl; or R a and R b together with the nitrogen to which they are attached are pyrrolidino, piperidino, morpholino, or thiomoφholino; wherein any aryl, or heteroaryl of R, and R 5 is optionally substituted with one or more (e.g., 1, 2, or 3) substituents independently selected from halo, nitro, cyano, hydroxy, trifluoromethyl, trifluoromethyl, trifluoromethoxy, (C r C 3 )alkoxy, (C,-C 3 )alkyl, (C r C 3 )alkanoyl, -S(O) 2 R c , or NR a R b ; wherein R c is (C,-C 3 )alkyl, or aryl or a pharmaceutically acceptable salt thereof; wherein said compound has a molecular volume of between about 300 and 500 A 3 . 2. The compound of formula I having the structure of formula II: (II) where R, is (C,-C 6 )alkyl, optionally substituted by 1, 2, or 3 substituents selected from the group consisting of halo, hydroxy, amino, (C,-C 6 )alkoxy, and (C,- C 6 )alkanoyloxy; R, is hydroxy, (C,-C 6 )alkoxy, (C,-C 6 )alkanoyloxy;R 3 is cyano, alkanoyl; R 6 is amino, hydroxy, cyano, nitro, (C,-C 6 )alkyl, halo(C,-C 6 )alkyl, (C,- C 6 )alkoxy, halo(C,-C 6 )alkoxy, (C r C 6 )alkanoyl, or (C,-C 6 )alkanoyloxy; and R 7 is H, NH 2 , CH 3 , OH, CF 3 , or halo; or a pharmaceutically acceptable salt thereof. 3. The compound of claim 2, having the formula III, IV, V, or VI: (III) where R 7 is H, NH 2 , CH 3 , OH, CF 3 , or halo. Preferably, halo is Br or Cl; (IV) here R 7 is H, NH 2 , CH 3 , OH, CF 3 , or halo. Preferably, halo is F or Cl; (V) where R 6 is NH-CH 3 or OCH 3 (VI) where R 2 is -CH 2 -CH 2 X and X is halo, preferably F; or R 2 is -CH 2 CF 3 ; or -N O R 2 is: 4. The compound of claim 1, having the structural formula: 5. A pharmaceutical composition comprising a compound of claim 1 and a pharmaceutically acceptable carrier. 6. A pharmaceutical composition comprising a compound of claim 2 and a pharmaceutically acceptable carrier. 7. A pharmaceutical composition comprising a compound of claim 3 and a pharmaceutically acceptable carrier. 8. A pharmaceutical composition comprising a compound of claim 4 and a pharmaceutically acceptable carrier. 9. A method for specifically inhibiting EGFR tyrosine kinase without inhibiting the Src family tyrosine kinases, Tec family tyrosine kinases, or Janus family tyrosine kinases, the method comprising contacting the EGFR with a compound of claim 1. 10. A method for specifically inhibiting EGFR tyrosine kinase without inhibiting the Src family tyrosine kinases, Tec family tyrosine kinases, or Janus family tyrosine kinases, the method comprising contacting the EGFR with a compound of claim 2. 11. A method for specifically inhibiting EGFR tyrosine kinase without inhibiting the Src family tyrosine kinases, Tec family tyrosine kinases, or Janus family tyrosine kinases, the method comprising contacting the EGFR with a compound of claim 3. 12. A method for specifically inhibiting EGFR tyrosine kinase without inhibiting the Src family tyrosine kinases, Tec family tyrosine kinases, or Janus family tyrosine kinases, the method comprising contacting the EGFR with a compound of claim 4. 13. A method for inhibiting EGFR tyrosine kinase in EGFR-expressing cells comprising administering to said EGFR-expressing cells a compound of claim 1. 14. The method of claim 13, wherein said EGFR-expressing cells are cancer cells, vascular smooth muscle cells, or endothelial cells involved in tumor angiogenesis. 15. A therapeutic method for treating a pathologic condition characterized by EGFR tyrosine kinase activity comprising administering to a mammal in need of such therapy, the compound of claim 1. 16. The method of claim 15, wherein said pathologic condition is cancer or an increased risk of cancer. 17. The method of claim 16, wherein the cancer is breast cancer, prostate cancer, lung cancer, melanoma, brain tumor, bladder cancer, or colon cancer. 18. The method of claim 16, wherein said increased risk of cancer is a genetic predisposition, an occupational predisposition, or a nutritional predisposition to cancer. 19. The method of claim 15, wherein said pathologic condition is atherosclerosis. 20. The method of claim 15, wherein said compound is the compound of claim 4. 21. A composite homology model for EGFR kinase domain constructed by the structural alignment of sequences of EGFR with sequences of known crystal structures of two or more other protein kinases, and comprising a planar triangular binding pocket having sides of approximately 15A x 12A x 12 A, a thickness of approximately 7 A, and a volume of approximately 600 A 3 . 22. An inhibitor of EGFR kinase, comprising a structure which fits the triangular binding pocket of claim 21.

Inventors: GHOSH SUTAPA, UCKUN FATIH M, ZHENG YAGUO, GHOSH, SUTAPA, UCKUN, FATIH, M., ZHENG, YAGUO
Applicants: PARKER HUGHES INST, PARKER HUGHES INSTITUTE
Application year: 2000
Main classification: C07C255
IPC codes: A61P35, C07C255, C07D295