COMPOUNDS HAVING CYTOKINE INHIBITORY ACTIVITY

Abstract: There are disclosed compounds of formula (I) and pharmaceutically acceptable salts thereof which exhibit utility for the treatment of cytokine mediated diseases such as arthritis.

Description: TITLE OF THE INVENTION COMPOUNDS HAVING CYTOKLNE TNHrBiTORY ACTIVITY BACKGROUND OF THE INVENTION The present invention relates to substituted lmidazole compounds which have cytokine inhibitory activity. Cytokme mediated diseases and cytokine inhibition, suppression and antagonism are used in the context of diseases or conditions in which excessive or unregulated production or activity of one or more cytokmes occurs. Examples of cytokines which are effected typically include Interleukιn-1 (IL-1), Interleukιn-6 (TL-6), Interleukιn-8 (IL-8) and Tumor Necrosis Factor (TNF). Interleukιn-1 (IL-1) and Tumor Necrosis Factor (TNF) are produced by a vaπety of cells which are involved in immunoregulation and other physiological conditions There are many disease states in which IL-1 is implicated. Examples are rheumatoid arthπtis, osteoarthπtis, endotoxemia, toxic shock syndrome, acute and chronic inflammatory diseases, such as the inflammatory reaction induced by endotoxin or inflammatory bowel disease; tuberculosis, atherosclerosis, muscle degeneration, cachexia, psoπatic arthπtis, Reiter's syndrome, rheumatoid arthπtis, gout, traumatic arthπtis, rubella arthπtis and acute synovitis. Recent evidence also links IL-1 activity to diabetes. Interleukm-1 has been demonstrated to mediate a vaπety of biological activities thought to be important in immunoregulation and other physiological conditions. [See, e.g., Dinarello et al., Rev. Infect. Disease, 6, 51 (1984)] The known biological activities of IL-1 include the activation of T helper cells, induction of fever, stimulation of prosta-glandm or collagenase production, neutrophil chemotaxis, induction of acute phase proteins and the suppression of plasma iron levels. Excessive or unregulated tumor necrosis factor (TNF) production or activity has been implicated in mediating or exacerbating rheumatoid arthπtis, rheumatoid spondyhtis, osteoarthntis, gouty arthπtis, and other arthπtic conditions, sepsis, septic shock, endotoxic shock, gram negative sepsis, toxic shock syndrome, adult respiratory distress syndrome, cerebral malaπa, chronic pulmonary inflammatory disease, sihcosis, pulmonary sarcosis, bone resorption diseases, reperfusion injury, graft v. host rejection, allograft rejections, fever and myalgia due to infection, cachexia secondary to infection or malignancy, cachexia secondary to acquired immune deficiency syndrome (AIDS), AIDS related complex (ARC), keloid formation, scar tissue formation, Crohn's disease, ulcerative colitis and pyresis Monokines, such as TNF, have also been shown to activate HIV replication in monocytes and/or macrophages [See Poll, et al , Proc Natl Acad Sci , 87 782-784 (1990)], therefore, inhibition of monok e production or activity aids in limiting HTV progression TNF has been implicated in vaπous roles with other viral infections, such as the cytomegalovirus (CMV), influenza virus and the herpes virus Interleukιn-6 (IL-6) is a cytokine effecting the immune system and hematopoiesis It is produced by several mammalian cell types m response to agents such as IL-1, and is correlated with disease states such as angiofolhcular lymphoid hyperplasia Interleukιn-8 (IL-8) is a chemotactic factor first identified and characteπzed in 1987 Many different names have been applied to IL-8, such as neutrophil attractant/activation protem-l (NAP-1), monocyte deπved neutrophil chemotactic factor (MDNCF), neutrophil activating factor (NAF), and T-cell lymphocyte chemotactic factor Like IL-1, IL-8 is produced by several cell types, including mononuclear cells, fibroblasts, endothehal cells and ketainocytes Its production is induced by IL-1, TNF and by hpopolysacchaπde (LPS) IL-8 stimulates a number of cellular functions in vitro It is a chemoattractant for neutrophils, T-lymphocytes and basophils It induces histamme release from basophils It causes lysozomal enzyme release and respiratory burst from neutrophils, and it has been shown to increase the surface expression of Mac-1 (CD1 lb/CD 18) on neutrophils without de novo protein synthesis There remains a need for compounds which are useful in treating cytokine mediated diseases, and as such, inhibit, suppress or antagonize the production or activity of cytokines such as IL-1, IL-6, IL-8 and TNF SUMMARY OF THE INVENTION The present invention relates to compound I of the formula wherein X is C j -C 6 alkyl, said alkyl group optionally substituted by 1-3 groups selected from halogen, hydroxy, CF 3 , NH2 and NO2, or a heterocychc group connected to the lmidazole πng by a direct bond or by C,-C 6 alkyl, R. and R 2 are independently hydrogen or C,-C 6 alkyl, said alkyl group optionally substituted by 1-3 groups selected from halogen, hydroxy, CF,, NH2 and NO2, or R. and R 9 taken together with the nitrogen atom represent an optionally substituted 4 to 10 membered non-aromatic heterocychc πng containing at least one N atom, and optionally containing 1-2 additional N atoms and 0-1 O or S atom, or R and X taken together represent an optionally substituted 4 to 10 membered non- aromatic heterocychc πng containing at least one N atom, and optionally containing 1-2 additional N atoms and 0-1 O or S atoms, R 3 is hydrogen or NH(C j -C 6 alkyl)aryl, R 4 , R < - and R 6 independently represent a member selected from the group consisting of hydrogen, halo, hydroxy, CF 3 NH~ NO 2 C,-C 6 alkyl, said alkyl group optionally substituted by 1-3 groups selected from halogen, hydroxy, CF 3 , NH2 and NO2, C , -Cg alkoxy, said alkoxy group optionally substituted by 1-3 groups selected from halogen, hydroxy, CF 3 , NH2 and NO2; C 3 -C 8 cycloalkyl, said cycloalkyl group optionally substituted by 1-3 groups selected from halogen, hydroxy, CF , NH2 and NO2 or aryl, said aryl group optionally substituted by 1-3 groups selected from halogen, hydroxy, CF 3 , NH2 and NO2; R 7 is hydrogen or C,-C 6 alkyl, said alkyl group optionally substituted by 1-3 groups selected from halogen, hydroxy, CF 3 , NH2 and NO2; or a pharmaceutically acceptable addition salt and/or hydrate thereof, or where applicable, a geometric or optical isomer or racemic mixture thereof. This invention also relates to a pharmaceutical composition which is comprised of a compound of formula I as defined above in combination with a pharmaceutically acceptable carrier. Also included in the invention is a method of treating a cytokine mediated disease in a mammal, comprising administering to a mammalian patient in need of such treatment an amount of a compound of formula I which is effective for treating said cytokine mediated disease. DETAILED DESCRIPTION OF THE INVENTION The present invention relates to compound I of the formula wherein X is j -C 6 alkyl, said alkyl group optionally substituted by 1-3 groups selected from halogen, hydroxy, CF 3 , NH2 and NO2; or a heterocychc group connected to the lmidazole πng by a direct bond or by C j -C 6 alkyl; R, and R 2 are independently hydrogen or C,-C 6 alkyl, said alkyl group optionally substituted by 1-3 groups selected from halogen, hydroxy, CF , NH2 and NO 2 ; or R. and R 2 taken together with the nitrogen atom represent an optionally substituted 4 to 10 membered non-aromatic heterocychc πng containing at least one N atom, and optionally containing 1-2 additional N atoms and 0-1 O or S atom; or R 2 and X taken together represent an optionally substituted 4 to 10 membered non- aromatic heterocychc πng containing at least one N atom, and optionally containing 1-2 additional N atoms and 0-1 O or S atoms; Q is CH or N; R 3 is hydrogen or NH(C,-C 6 alkyl)aryl, R 4 , R- and R 6 independently represent a member selected from the group consisting of hydrogen, halo, hydroxy, CF 3 NH 2 NO 2 C j -C 6 alkyl, said alkyl group optionally substituted by 1-3 groups selected from halogen, hydroxy, CF , NH2 and NO2, C,-C,- alkoxy, said alkoxy group optionally substituted by 1-3 groups selected from halogen, hydroxy CF 3 , NH2 and NO2; C 3 -C 8 cycloalkyl, said cycloalkyl group optionally substituted by 1-3 groups selected from halogen, hydroxy, CF,, NHo and NO2 or aryl, said aryl group optionally substituted by 1-3 groups selected from halogen, hydroxy, CF 3 , NH2 and NO2; R 7 is hydrogen or C,-C 6 alkyl, said alkyl group optionally substituted by 1-3 groups selected from halogen, hydroxy, CF 3 , NH2 and NO2; or a pharmaceutically acceptable addition salt and/or hydrate thereof, or where applicable, a geometπc or optical isomer or racemic mixture thereof. This invention also relates to a pharmaceutical composition which is compπsed of a compound of formula I as defined above in combination with a pharmaceutically acceptable earner. Also included in the invention is a method of treating a cytokine med ted disease in a mammal, compπsing administeπng to a mammalian patient in need of such treatment an amount of a compound of formula I which is effective for treating said cytokine mediated disease. In a preferred embodiment, there is disclosed a compound of the formula or a pharmaceutically acceptable salt thereof, wherein: Q is CH; X is C1 -C6 alkyl; or R 2 and X taken together represent an azetidine group; R Λ , R- and R^ are independently hydrogen or CF 3 ; R7 is CH3; Ri and R 2 are independently hydrogen or C,-C 6 alkyl; or R , and R 2 taken together with the nitrogen atom form a morpholine group. Representative species falling within the present invention include the following: Unless otherwise stated or indicated, the following definitions shall apply throughout the specification and claims The term "alkyl" refers to a monovalent alkane (hydrocarbon) deπved radical containing from 1 to 15 carbon atoms unless otherwise defined. It may be straight or branched, and when of sufficient size, e.g , C3-15 may be cyclic Prefeπed straight or branched alkyl groups include methyl, ethyl, propyl. isopropyl, butyl and t-butyl. Preferred cycloalkyl groups include cyclopropyl, cyclopentyl and cyclohexyl. Alkyl also includes an alkyl group substituted with a cycloalkyl group, such as cyclopropylmethyl. Alkyl also includes a straight or branched alkyl group The alkylene and monovalent alkyl portιon(s) of the alkyl group can be attached at any available point of attachment to the cycloalkylene portion When substituted alkyl is present, this refers to a straight, branched or cyclic alkyl group as defined above, substituted with 1-3 groups as defined with respect to each vaπable. The term "aryl" refers to aromatic πngs e g., phenyl, substituted phenyl and like groups as well as πngs which are fused, e.g., naphthyl and the like. Aryl thus contains at least one πng having at least 6 atoms, with up to two such πngs being present, containing up to 10 atoms therein, with alternating (resonating) double bonds between adjacent carbon atoms The preferred aryl groups are phenyl and naphthyl. Aryl groups may likewise be substituted as defined below. Preferred substituted aryls include phenyl or naphthyl substituted with one or two groups. The terms "heterocycloalkyl" and "heterocyclyl" refer to a cycloalkyl group (nonaromatic) in which one of the carbon atoms in the πng is replaced by a heteroatom selected from O, S(O)y or N, and in which up to three additional carbon atoms may be replaced by said heteroatoms. When three heteroatoms are present in the heterocycle, they are not all linked together. Examples of heterocyclyls are pipeπdmyl, morpholinyl, azetidinyl, pyrrolidmyl, tetrahydrofuranyl, imidazolmyl, piperazmyl, pyrohdιn-2-one, pipeπdm- 2-one and the like. The term "halogen" or "halo" is intended to include fluoπne, chloπne, bromine and iodine. As used herein, the term "composition" is intended to encompass a product compπsing the specified ingredients in the specified amounts, as well as any product which results, directly or indirectly, from combination of the specified ingredients in the specified amounts In addition, it is well known to those skilled in the art that many of the foregoing heterocychc groups can exist more than one tautomeπc form. It is intended that all such tautomers be included with the ambit of this invention. The optical isomeπc forms, that is mixtures of enantiomers, e.g., racemates, or diastereomers as well as individual enantiomers or diastereomers of the instant compound are included. These individual enantiomers are commonly designated according to the optical rotation they effect by the symbols (+) and (-), (L) and (D), (1) and (d) or combinations thereof. These isomers may also be designated according to their absolute spatial configuration by (S) and (R), which stands for sinister and rectus, respectively. The individual optical isomers may be prepared using conventional resolution procedures, e.g., treatment with an appropπate optically active acid, separating the diastereomers and then recoveπng the desired isomer. In addition, the individual optical isomers may be prepared by asymmetπc synthesis Additionally, a given chemical formula or name shall encompass pharmaceutically acceptable addition salts thereof and solvates thereof, such as hydrates. The compounds of the present invention, while effective themselves, may be formulated and administered m the form of their pharmaceutically acceptable addition salts for purposes of stability, convenience of crystallization, increased solubility and other desirable properties. The compounds of the present invention may be administered m the form of pharmaceutically acceptable salts. The term "pharmaceutically acceptable salt" is intended to include all acceptable salts. Examples of acid salts are hydrochloπc, nitπc, sulfuπc, phosphoπc, formic, acetic, tπfluoroacetic, propionic, maleic, succmic, malonic, methane sulfomc and the like which can be used as a dosage form for modifying the solubility or hydrolysis characteπstics or can be used in sustained release or prodrug formulations. Depending on the particular functionality of the compound of the present invention, pharmaceutically acceptable salts of the compounds of this invention include those formed from cations such as sodium, potassium, aluminum, calcium, lithium, magnesium, zinc, and from bases such as ammonia, ethylenediamme, N-methyl-glutamme, lysine, arginine, ornithme, choline, N,N'-dιbenzylethylenedιamιne, chloroprocaine, diethanolamme, procame, N- benzylphenethylamine, diethylamine, piperazine, tπs(hydroxymethyl)ammomethane, and tetramethyl-ammonium hydroxide. These salts may be prepared by standard procedures, e.g. by reacting a free acid with a suitable organic or inorganic base, or alternatively by reacting a free base with a suitable organic or inorganic acid. Also, in the case of an acid (-COOH) or alcohol group being present, pharmaceutically acceptable esters can be employed, e.g. methyl, ethyl, butyl, acetate, maleate, pivaloyloxymethyl, and the like, and those esters known in the art for modifying solubility or hydrolysis characteπstics for use as sustained release or prodrug formulations. The compounds of the present invention may have chiral centers other than those centers whose stereochemistry is depicted in formula I, and therefore may occur as racemates, racemic mixtures and as individual enantiomers or diastereomers, with all such isomeπc forms being included in the present invention as well as mixtures thereof. Furthermore, some of the crystalline forms for compounds of the present invention may exist as polymorphs and as such are intended to be included m the present invention. In addition, some of the compounds of the instant invention may form solvates with water or common organic solvents Such solvates are encompassed within the scope of this invention. The term "TNF mediated disease or disease state" refers to disease states in which TNF plays a role, either by production or increased activity levels of TNF itself, or by causing another cytokine to be released, such as but not limited to IL-1 or IL-6 A disease state in which IL-1, for instance is a major component, and whose production or action, is exacerbated or secreted in response to TNF, would therefore be considered a disease state mediated by TNF The term "cytokine" as used herein means any secreted polypeptide that affects the functions of cells and is a molecule which modulates interactions between cells in the immune, inflammatory or hematopoietic response A cytokine includes, but is not limited to, monokines and lymphokines regardless of which cells produce them Examples of cytokines include, but are not limited to, Interleukιn-1 (IL-1), Interleukιn-6 (IL-6), Interleukιn-8 (IL-8), Tumor Necrosis Factor-alpha (TNF- α) and Tumor Necrosis Factor-beta (TNF-β). By the term "cytokine interfenng or cytokine suppresive amount" is meant an effective amount of a compound of formula I which will cause a decrease in the in vivo activity or level of the cytokine to normal or sub-normal levels, when given to the patient for the prophylaxis or therapeutic treatment of a disease state which is exacerbated by, or caused by, excessive or unregulated cytokine production or activity. The compounds of the invention are prepared by the following general reaction scheme. All substituents are as defined above unless indicated otherwise. The preparation of specific compounds is illustrated in the examples section. Scheme 1 t-BuONO coupling ammonium trifluoroacetate The compounds of formula 1 can be used in the prophylactic or therapeutic treatment of disease states in mammals which are exacerbated or caused by excessive or unregulated cytokines, e.g., IL-1, IL-6, IL-8 or TNF. Because the compounds of formula I inhibit cytokines, the compounds are useful for treating diseases in which cytokine presence or activity is implicated, such as rheumatoid arthπtis, rheumatoid spondylitis, osteoarthπtis, gouty arthπtis and other arthπtic conditions. The compounds of formula I are useful to treat disease states mediated by excessive or unregulated TNF production or activity. Such diseases include, but are not limited to sepsis, septic shock, endotoxic shock, gram negative sepsis, toxic shock syndrome, adult respiratory distress syndrome, cerebral malaπa, chronic pulmonary inflammatory disease, sihcosis, pulmonary sarcoidosis, bone resorption diseases, such as osteoporosis, reperfusion injury, graft v. host rejection, allograft rejection, fever, myalgia due to infection, cachexia secondary to infection or malignancy, cachexia secondary to acquired immune deficiency syndrome (AIDS). ADDS, ARC (AIDS related complex), keloid formation, scar tissue formation, Crohn's disease, ulcerative colitis, pyresis, AIDS and other viral infections, such as cytomegalovirus (CMV), influenza virus, and the herpes family of viruses such as Herpes Zoster or Simplex I and π. The compounds of formula I are also useful topically in the treatment of inflammation such as in the treatment of rheumatoid arthπtis, rheumatoid spondylitis, osteoarthπtis, gouty arthπtis and other arthπtic conditions; inflamed joints, eczema, psoπasis or other inflammatory skm conditions such as sunburn; inflammatory eye conditions including conjunctivitis; pyresis, pain and other conditions associated with inflammation. The compounds of formula I are also useful in treating diseases characteπzed by excessive IL-8 activity. These disease states include psoπasis, inflammatory bowel disease, asthma, cardiac and renal reperfusion injury, adult respiratory distress syndrome, thrombosis and glomerulonephπtis. The invention thus includes a method of treating psoπasis, inflammatory bowel disease, asthma, cardiac and renal reperfusion injury, adult respiratory distress syndrome, thrombosis and glomerulonephπtis, m a mammal in need of such treatment, which compπses administeπng to said mammal a compound of formula I in an amount which is effective for treating said disease or condition. When administered to a patient for the treatment of a disease in which a cytokine or cytokines are implicated, the dosage used can be vaπed with wide limits, depending upon the type of disease, the age and general condition of the patient, the particular compound administered, the presence or level of toxicity or adverse effects expeπenced with the drug and other factors. A representative example of a suitable dosage range is from as low as about 0.01 mg/kg to as high as about 100 mg/kg. However, the dosage administered is generally left to the discretion of the physician. The methods of treatment can be earned out by dehveπng the compound of formula I parenterally. The term 'parenteral' as used herein includes intravenous, intramuscular, or intrapentoneal administration. The subcutaneous and intramuscular forms of parenteral administration are generally preferred The instant invention can also be earned out by dehvenng the compound of formula I subcutaneously, intranasally, mtrarectally, transdermally or mtravagmally The compounds of formula I may also be administered by inhalation. By 'inhalation' is meant intranasal and oral inhalation administration. Appropnate dosage forms for such administration, such as an aerosol formulation or a metered dose inhaler, may be prepared by convention techniques. The invention also relates to a pharmaceutical composition compπsing a compound of formula I and a pharmaceutically acceptable earner. The compounds of formula I may also be included in pharmaceutical compositions in combination with a second therapeutically active compound. The pharmaceutical earner employed may be, for example, either a solid, liquid or gas. Exemples of solid earners include lactose, terra alba, sucrose, talc, gelatin, agar, pectin, acacia, magnesium stearate, steaπc acid and the like. Exemples of liquid earners are syrup, peanut oil, olive oil, water and the like Examples of gaseous earners include carbon dioxide and nitrogen. Similarly, the earner or diluent may include time delay matenal well known in the art, such as glyceryl monostearate or glyceryl distearate, alone or with a wax. A wide vaπety of pharmaceutical dosage forms can be employed. If a solid dosage is used for oral administration, the preparation can be in the form of a tablet, hard gelatin capsule, troche or lozenge The amount of solid earner will vary widely, but generally will be from about 0.025 mg to about 1 g. When a liquid dosage form is desired for oral administration, the preparation is typically in the form of a syrup, emulsion, soft gelatin capsule, suspension or solution. When a parenteral dosage form is to be employed, the drug may be in solid or liquid form, and may be formulated for administration directly or may be suitable for reconstitution. Topical dosage forms are also included. Examples of topical dosage forms are solids, liquids and semi-solids. Solids would include dusting powders, poultices and the like. Liquids include solutions, suspensions and emulsions. Semi- sohds include creams, ointments, gels and the like The amount of a compound of formula I used topically will, of course, vary with the compound chosen, the nature and seventy of the condition, and can be vaned in accordance with the discretion of the physician A representative, topical, dose of a compound of formula I is from as low as about 0 01 mg to as high as about 2.0 g, administered one to four, preferably one to two times daily The active ingredient may compnse, for topical administration, from about 0.001% to about 10% w/w. Drops according to the present invention may compnse steπle or non- steπle aqueous or oil solutions or suspensions, and may be prepared by dissolving the active ingredient in a suitable aqueous solution, optionally including a bactericidal and/or fungicidal agent and/or any other suitable preservative, and optionally including a surface active agent. The resulting solution may then be clarified by filtration, transferred to a suitable container which is then sealed and sterilized by autoclaving or maintaining at 98-100°C for half an hour. Alternatively, the solution may be sterilized by filtration and transferred to the container aseptically. Examples of bactericidal and fungicidal agents suitable for inclusion in the drops are phenylmercuric nitrate or acetate (0.002%), benzalkonium chloride (0.01%) and chlorhexidine acetate (0.01%). Suitable solvents for the preparation of an oily solution include glycerol, diluted alcohol and propylene glycol. Lotions according to the present invention include those suitable for application to the skin or eye. An eye lotion may comprise a sterile aqueous solution optionally containing a bactericide and may be prepared by methods similar to those for the preparation of drops. Lotions or liniments for application to the skin may also include an agent to hasten drying and to cool the skin, such as an alcohol or acetone, and/or a moisturizer such as glycerol or an oil such as castor oil or arachis oil. Creams, ointments or pastes according to the present invention are semi-solid formulations of the active ingredient for external application. They may be made by mixing the active ingredient in finely-divided or powdered form, alone or in solution or suspension in an aqueous or non-aqueous liquid, with a greasy or non- greasy base. The base may comprise hydrocarbons such as hard, soft or liquid paraffin, glycerol, beeswax, a metallic soap; a mucilage; an oil of natural origin such as almond, corn, arachis, castor or olive oil; wool fat or its derivatives, or a fatty acid such as stearic or oleic acid together with an alcohol such as propylene glycol or macrogels. The formulation may incorporate any suitable surface active agent such as an anionic, cationic or non-ionic surfactant such as sorbitan esters or polyoxyethylene derivatives thereof. Suspending agents such as natural gums, cellulose derivatives or inorganic materials such as silicas, and other ingredients such as lanolin may also be included. The following examples illustrate the preparation of some of the compounds of the invention and are not to be construed as limiting the invention disclosed herein. EXAMPLE 1 (S)-l-phenyl-N-{4-r2-(2-amino-ethyl)-3-methyl-5-(3-trifluoromethyl-phenyl)-3H- imidazol-4-yll-pyridin-2-yl|-ethylamine Step 1A: 2-(2-Fluoropyridin-4-yl)-l-(3-trifluoromethylphenyl)- ethanone To a suspension of N,O-dimethylhydroxylamine hydrochloride (58.2 g, 0.60 mol) in dichloromethane (1 L) at 0°C, under argon, was added 3- trifluoromethylbenzoyl chloride (104.0 g, 0.50 mol), followed by a slow addition of triethylamine (152.3 mL, 1.09 mol). The reaction was aged for 30 min. at 5°C and then allowed to warm to room temperature. TLC (1:1, ethyl acetate / hexane) showed the reaction to be complete. The reaction was washed with 5% aqueous citric acid (500 mL) and 5% aqueous sodium bicarbonate. The aqueous extracts were back extracted with methylene chloride (100 mL) and the combined methylene chloride extracts were dried over sodium sulfate, filtered and concentrated to an oil. The oil was redissolved in toluene (2 X 100 mL) and evaporated in vacuo to afford the Weinreb amide (114.7 g). Η NMR (300 MHz, CDC13) d 7.98 (s, 1 H, Ar), 7.89 (d, J = 7.8 Hz, 1 H, Ar), 7.72 (d, J = 7.8 Hz, 1 H, Ar), 7.55 (t, J = 7.8 Hz, 1 H, Ar), 3.55 (s, 3 H, CH 3 O), 3.39 (s, 3 Η, CH 3 N). To a stirring solution of diisopropylamine (17.69 mL, 0.135 mol) in anhydrous TΗF (200 mL) at -78°C, under argon, was added n-butyllithium (54.0 mL, 2.5M m hexane, 0.135 mol), followed after 5 min. by a solution of 2-fluoro-4- methylpyndme (10 g, 0.090 mol) in anhydrous THF (20 mL). After stirπng for 15 mm. at -78°C, a solution of N-methoxy-N-methyl-3-tπfluoromethylbenzamιde (23.08 g, 0.099 mol) in anhydrous THF (10 mL) was added to the reaction mixture which was then stirred for 5 mm., and allowed to warm to 0°C. The reaction was quenched with water (400 mL), and extracted with ethyl acetate (3x200 mL). The ethyl acetate extracts were combined, dπed over anhydrous sodium sulfate, filtered, and concentrated to an oil which was chromatographed on silica gel (1 kg), elut g with 20% ethyl acetate in hexane to give 21.6 g of the title compound. Η NMR (300 MHz, CDC1 3 ) d 8.25 (s, 1 H, Pyr), 8.20 (d, J = 5.1 Hz, 1 H, Pyr), 8.18 (d, J = 9.3 Hz, 1 H, Pyr), 7.88 (d, J =7.8 Hz, Ar), 7.67 (t, J = 7.8 Hz, 1 H, Ar), 7.09 (d, / = 5.1 Hz, 1 H, Ar), 6 86 (s, 1 H, Ar), 4 37 (s, 2 H, PyrCH 2 C). Step IB: l-(2-Fluoropyπdm-4-yl)-2-(3-tπfluoromethylphenyl)- ethane-1.2-dιone 1-oxιme To a mixture of 2-(2-fluoropyπdιn-4-yl)-l-(3- tnfluoromethylphenyl)ethanone (10.80 g, 0.038 mol) in ethanol (200 mL), at -10°C, under argon, was added tert-butyl nitnte (5.0 mL, 0 042 mol) and hydrogen chlonde (12.2 mL, 2.5M in ethanol, 0.031 mol) dropwise while maintaining the temperature below -5°C. Upon completion of addition, the reaction was allowed to warm to RT for 2 hrs. The reaction mixture was then concentrated in vacuo , diluted with water (100 mL), basified with saturated sodium bicarbonate (200ml), and extracted with ethyl acetate (3x400 mL) The combined organic layers were washed with water (300 mL), dned with bπne (300 mL) and anhydrous sodium sulfate, filtered and concentrated to yield 11.4g of the title compound, as an oil. Η NMR (300 MHz, CDC1 3 ) d 8.31 (s, 1 H, Ar), 8.29 (d, J = 5.3 Hz, 1 H, Pyr), 8.24 (d, J = 7.8 Hz, 1 H, Ar), 7 92 (d, J = 8.1 Hz, 1 H, Ar), 7.71 (t, J = 7.8 Hz, 1 H, Ar), 7.40 (d, J = 5.1 Hz, 1 H, Pyr), 7.23 (s, 1 H, Pyr). Step 1C: 2-amιno-2-(2-fluoropyndιn-4-yl)-l-(3-tπfluoromethyl- phenyD-ethanol 10% Palladium on carbon (3.0g) was added to a solution of l-(2-fluoropyπdm-4-yl)-2-(3-tnfluoromethylphenyl)-ethane-l,2-dιone 1-oxime (8.0g, 27 mmol) in ethanol (400 mL) at ambient temperature. The reaction vessel was vacuum purged, refilled with hydrogen and vigorously stirred for 10 hrs. After the reaction was complete, the solution was filtered through a pad of celite, and concentrated to give a solid. The residue was purified by recrystallization from methylene chloride and hexane to give 7.4g of the title compound, m.p. 128-129°C. Η NMR (300 MHz, CD 3 OD) d 8.01 (d, J = 5.0 Hz, 1 H, Ar), 7.53 (m, 1 H, Ar), 7.49 (m, 2 H, Ar), 7.43 (s, 1 H, Ar), 7.06 (d, J = 5.0 Hz, 1 H, Ar), 6.86 (s, 1 H, Ar), 4.96 (d, J = 5.0 Hz, 1 H, CH), 4.12 (d, J = 5.0 Ηz, 1 Η, CH). Step ID: 2-(2-fluoropyridin-4-yl)-2-methylamino-l-(3-trifluoromethyl- phenvP-ethanol 2-Amino-2-(2-fluoropyridin-4-yl)-l-(3-trifluoromethyl-phenyl)-ethanol (6 g, 20 mmol) was dissolved in ethyl formate (80 mL) and heated to reflux for 10 hrs under an argon atmosphere. The reaction mixture was cooled to ambient temperature and concentrated to yield the formamide as an oil. Η NMR (300 MHz, CD 3 OD) d 8.06 (m, 2 H, Ar), 7.55 (m, 3 H, Ar), 7.05 (m, 1 H, Ar), 5.22 (d, 7 = 5.7 Hz, 1 H, CH), 5.10 (d, J = 5.7 Ηz, 1 Η, CH). The formamide was azeotroped with toluene (2 x 100 mL) to remove trace amounts of ethyl formate and water. Reduction to the desired compound was carried out by drop-wise addition of borane-tetrahydrofuran complex (60 mL of a 1.0 M solution in TΗF, 60 mmol) to a solution the formamide product (20 mmol) in TΗF (60 mL) at ambient temperature. After 1 hr, the reaction was quenched by slow addition of the organic mixture to a vigorously stirring solution of aqueous hydrochloric acid (2.0 M, 500 mL). Stirring was continued for 3 h to assure complete dissociation of boron complexes. A solution of aqueous sodium hydroxide (10 M) was then added until a pΗ of 11 was achieved. The resulting mixture was extracted with ethyl acetate (2 x 300 mL), dried (sodium sulfate), and concentrated. The resulting residue was purified by filtration though a pad of silica gel (using 5% methanol and 0.5% ammonium hydroxide mixture in methylene chloride) to yield 5.3g of a solid: mp 99 - 102 °C. Η NMR (300 MHz, CD 3 OD) d 7.99 (d, J = 5.1 Hz, 1 H, Ar), 7.50 (m, 1 H, Ar), 7.45 (m, 2 H, Ar), 7.38 (s, 1 H, Ar), 7.02 (m, 1 H, Ar), 6.83 (s, 1 H, Ar), 5.09 (d, J = 4.8 Hz, 1 H, CH), 3.85 (d, J = 4.8 Ηz, 1 Η, CH), 2.22 (s, 3 Η, CH 3 ). Step IE : 3-(Benzyloxycarbonyl-amιno)-N-[l-(2-fluoro-pyπdm- 4-yl)-2-hydroxy-2-(3-tπfluoromethyl-phenyl)-ethyl]- N-methyl-propionamide l-Ethyl-3-(3-dιmethylamιnopropyl)carbodnmιde hydrochloπde (0.61 g, 3.2 mmol) was added to a solution of 2-(2-fluoro-pyπdιn-4-yl)-2-methylammo-l- (3-tπfluoromethyl-phenyl)-ethanol (0 50 g, 1.6 mmol), amιno)-propanoιc acid (0.53 g, 2 4 mmol), tnethylamine (0.89 mL, 6 4 mmol), and 1- hydroxy-7-azabenzotnazole (0.43 g, 3 9 mmol) in dimethylformamide (10 mL) at ambient temperature. After 2 hrs stirπng at ambient temperature, ethyl acetate was added (200 mL) followed by aqueous citπc acid (50 mL of a 10% solution). The organic layer was then washed with aqueous sodium bicarbonate (50 mL of a sat. solution) and water (3 x 30 mL), dπed (sodium sulfate), filtered, and concentrated. The crude residue was passed though a pad of silica gel (50% ethyl acetate in hexane) to remove minor impunties and yield 1.4g of an oil. 'Η NMR (300 MHz, CD OD) d 8.17 (d, = 6.1 Hz, 1 H, Pyr), 8.01 (s, 1 H, Ar), 7.67 (d, J = 7.8 Hz, 1 H, Ar), 7.61 (d, J = 7.8 Hz, 1 H, Ar), 7.48 (t, J = 7.8 Hz, 1 H, Ar), 7.42 - 7.32 (m, 5 H, Ar), 7.19 (d, 7 = 7.1 Hz, 1 H, Pyr), 7.05 (s, 1 H, Pyr), 5.61 (d, J = 8.8 Hz, 1 H, PhCHOH), 5.40 (d, J = 8.8 Hz, 1 H, PhCHN), 5.03 (s, 2 Η, PhCH 2 O), 3.42 - 3.25 (m, 4 Η, ΗNCH 2 CH 2 ), 2.36 (s, 3 Η, CH 3 N). Step IF: N-benzyloxycarbonyl-2-[5-(2-fluoro-pyndιn-4-yl)-l-methyl- 4-(3-tnfluoromethyl-phenyl)-lH-ιmιdazol-2-yl1-ethylamιne Oxalyl chlonde (0.34 mL, 3.9 mmol) was added to a solution of dimethyl sulfoxide (0.41 mL, 5.8 mmol) in methylene chlonde (10 mL) at -78°C. After 20 mm. at -78 °C, 3-(benzyloxycarbonyl-amιno)-N-[l-(2-fluoro-pyπdιn-4-yl)-2- hydroxy-2-(3-tnfluoromethyl-phenyl)-ethyl]-N-methyl-propιonamιde (1.0 g, 1.9 mmol) in methylene chloπde (10 mL) was added and the reaction solution was stirred at -78°C for 2 hrs. Tnethylamine (1.3 mL, 9.6 mmol) was added and the cooling bath was removed. The solution was diluted with ethyl acetate (150 mL), washed with aqueous ammonium chloride (75 mL) and brine (75 mL), dried (sodium sulfate), and concentrated. Some non-polar impurities were removed by passing the residue through silica gel, eluting with 50% ethyl acetate in hexane. The ketone was then transferred to a flask containing anhydrous ammonium trifluoroacetate (4 g) using a minimum amount of ether-methylene chloride. The mixture was placed under vacuum to remove the solvent, and then placed in a preheated oil bath (150 °C). Once all solid had melted and efficient stirring was achieved (approx. 10 min.) the formation of the imidazole was found to be complete. The heating bath was removed and the solids were partitioned between ethyl acetate and water (150/50 mL). The organic layer was dried (sodium sulfate), filtered, and concentrated. The product was purified by filtration through silica gel (imidazole is the least polar component) using ethyl acetate to yield 735 mg of a foam. Η NMR (300 MHz, CD 3 OD) d 8.29 (d, J = 5.4 Hz, 1 H, Pyr), 7.69 (s, 1 H, Ar), 7.53 (d, J = 7.8 Hz, 1 H, Ar), 7.52 (d, J = 7.8 Hz, 1 H, Ar), 7.45 (t, J = 7.8 Hz, 1 H, Ar), 7.35 - 7.22 (m, 6 H, Ar), 7.05 (s, 1 H, Pyr), 5.03 (s, 2 H, PhCH 2 O), 3.55 (t, J = 6.8 Ηz, 2 Η, ΗNCH 2 CΗ 2 ), 3.51 (s, 3 H, CH 3 N), 3.03 (t, J = 6.7 Ηz, 2 Η, CCH 2 CΗ 2 ). Step 1G: (S)-l-phenyl-N-{4-[2-(2-(benzyloxycarbonyl-amino)- ethyl)-3-methyl-5-(3-trifluoromethyl-phenyl)-3H- imidazol-4-yl]-pyridin-2-yl )-ethylamine N-Benzyloxycarbonyl-2-[5-(2-fluoro-pyridin-4-yl)-l-methyl- 4-(3-trifluoromethyl-phenyl)-lH-imidazol-2-yl]-ethylamine (0.35 g, 0.70 mmol) was dissolved in S-(-)-a-methylbenzylamine (0.85 g, 7.0 mmol) and the reaction mixture was heated to 150 °C for 15 hrs. The heating bath was removed and the contents of the flask were partitioned between ethyl acetate (100 mL) and pΗ 4.5 buffer (50 mL composed of 10% citric acid that was treated with IO N sodium hydroxide to achieve a pΗ of 4.5). The organic layer was dried (sodium sulfate), filtered, and concentrated. The residue was purified by silica gel chromatography to yield 315 mg of the desired product as an oil. *Η NMR (300 MHz, CD 3 OD) d 7.99 (d, J = 5.4 Hz, 1 H, Pyr), 7.69 (s, 1 H, Ar), 7.49 (d, J = 8.8 Hz, 1 H, Ar), 7.47 (d, J = 9.3 Hz, 1 H, Ar), 7.36 (t, J = 7.7 Hz, 1 H, Ar). 7.33 - 7.13 (m, 10 H, Ar), 6.43 (d, J = 5.4 Hz, 1 H, Pyr), 6.33 (s, 1 H, Pyr), 5.03 (s, 2 H, PhCH 2 O), 4.81 (q, 7 = 6.8 Ηz, 1 Η, PhCHCΗ 3 ), 3.50 (t, 7 = 6.8 Hz, 2 H, HNCH 2 CH 2 ), 3.32 (s, 3 H, CH 3 N), 2.98 (t, 7 = 6.7 Hz. 2 H, CCH 2 CH 2 ), 1.48 (d, 7 = 6.8 Hz, 3 H, PhCHCHΛ Step 1H: (S)-l-phenyl-N-{4-[2-(2-amino-ethyl)-3-methyl-5- (3-trifluoromethyl-phenyl)-3H-imidazol-4-yl]-pyridin- 2-yl}-ethylamine A solution of (S)-l-phenyl-N-{4-[2-(2-(benzyloxycarbonyl-amino)- ethyl)-3-methyl-5-(3-trifluoromethyl-phenyl)-3H-imidazol-4-y]]-pyridin-2-yl}- ethylamine (0.20 g, 0.33 mmol) in absolute ethanol (10 mL) was flushed with argon before 10% palladium on activated carbon (0.07 g) was added. This reaction mixture, flushed with hydrogen gas, was vigorously stirred under one atmosphere of hydrogen for 5 hrs, then filtered and concentrated. The residue was purified by silica gel chromatography to yield 146 mg of the desired product as an oil. Η NMR (300 MHz, CD 3 OD) d 7.99 (d, 7 = 5.4 Hz, 1 H, Pyr), 7.70 (s, 1 H, Ar), 7.51 (d, 7 = 7.6 Hz, 1 H, Ar), 7.46 (d, 7 = 7.8 Hz, 1 H, Ar), 7.37 (t, 7 = 7.7 Hz, 1 H, Ar), 7.32 - 7.14 (m, 5 H, Ar), 6.47 (d, 7 = 5.4 Hz, 1 H, Pyr), 6.35 (s, 1 H, Pyr), 4.81 (q, 7 = 7.1 Hz, 1 H, PhCHCH 3 ), 3.35 (s, 3 H, CH 3 N), 3.06 (t, 7 = 6.7 Hz, 2 H, CH 2 CH 2 NH 2 ), 2.93 (t, 7 = 7.0 Hz, 2 H, CCH 2 CH 2 ), 1.48 (d, 7 = 6.8 Hz, 3 H, PhCHCT ). EXAMPLE 2 (S)-l-Phenyl-N-{4-r2-((R)-l-amιno-ethyl)-3-methyl-5-(3-trifluoromethyl-phenyl)-3H- ιmιdazol-4-yl1-pyndιn-2-yl }-ethylamιne The compound was prepared as descnbed in example 1, replacing 3- (benzyloxycarbonnyl-ammo)-propanoιc acid with N-Cbz-D-Alanme in step IE. 'Η NMR (300 MHz, CD 3 OD) d 8.00 (d, 7 = 5.4 Hz, 1 H, Pyr), 7.72 (s, 1 H, Ar), 7.51 (d, 7 = 9.1 Hz, 1 H, Ar), 7.48 (d, 7 = 9.2 Hz, 1 H, Ar), 7.37 (t, 7 = 7.7 Hz, 1 H, Ar), 7.31 - 7.22 (m, 4 H, Ar), 7.16 (t, 7 = 6.8 Hz, 1 H, Ar), 6.46 (m, 1 H, Pyr), 6.33 (s, 1 H, Pyr), 4.80 (q, 7 = 6.3 Hz, 1 H, PhCHCH 3 ), 4.26 (q, 7 = 6.6 Hz, 1 H, NH 2 CHCH 3 ), 3 41 (s, 3 H, NCH 3 ), 1.53 (d, 7 = 6.8 Ηz, 3 Η, PhCΗCH 3 ), 1.48 (d, 7 = 6.8 Ηz, 3 Η, NΗ 2 CΗCH 3 ); MS (FAB) Calcd for C 26 Η 26 F 3 N 5 (M + H + ) 466.2, found 466.3 EXAMPLE 3 (S)-l-Phenyl-N-{4-[2-(azetιdιn-3-yl-methyl)-3-methyl-5-(3-tnfluoromethyl-phenyl)- jH-ιmιdazol-4-yll-pyπdιn-2-yl }-ethylamιne Compound was prepared as descnbed in example 1, replacing 3- (benzyloxycarbonnyl-amιno)-propanoιc acid with 3-carboxymethyl-azetιdme-l- carboxyhc acid benzyl ester (synthesis descnbed below) m step E. J H NMR (300 MHz, CD 3 OD) d 7.99 (d, 7 = 5.1 Hz, 1 H, Pyr), 7.67 (s, 1H, Ar), 7.49 (d, 7 = 8.3 Hz, 1 H, Ar), 7.46 (d, 7 = 9.5 Hz, 1 H, Ar), 7.36 (t, 7 = 7 7 Hz, 1 H, Ar), 7.32 - 7.13 (m, 5 H, Ar), 6.45 (d, 7 = 5.1 Hz, 1 H, Pyr), 6.33 (s, 1H, Pyr), 4.80 (q, 7 = 6.8 Hz, 1 H, PhCHCH 3 ), 3.94 (m, 2 H, CHCH 2 NH), 3.69 (m, 2 H, CHCH 2 NH), 3.34 (s, 3 H, NCH 3 ), 3.10 (d, 7 = 7.6 Hz, 2 H, CCH 2 CH), 3.04 (m, 1 H, CH 2 CHCH 2 ), 1.48 (d, 7 = 6.8 Hz, 3 H, PhCHCH 3 ); MS (FAB) Calcd for C 28 Η 2g F 3 N 5 (M + H + ) 492.2, found 492.3 Step 3A: l-Benzhydryl-azetιdιn-3-ol hydrochlonde A mixture of benzhydrylamme (56 g, 306 mmol), and epichlorohydπn (24 mL, 306 mmol) in methanol (150 mL) was stirred at 24°C under argon for 3 days, then refluxed for 3 days. This reaction mixture was concentrated in vacuo, the residue stirred in acetone (500 mL) and filtered to give the crystaline salt as the title compound (78 g). Step 3B: (l-Benzhydryl-azetιdιn-3-ylιdene)-acetιc acid ethyl ester To a stirπng solution of DMSO (5.3 mL, 75 mmol) m methylene chloπde (100 mL) at -78°C under argon was added oxalyl chloπde (4.6 mL, 53 mmol) dropwise. After stirπng for 30 mm., a solution of l-benzhydryl-azetιdm-3-ol hydrochlonde (12) (10 g, 36 mmol) in methylene chlonde (10 mL) was added dropwise. The reaction mixture was stirred for lhr at -78°C before tnethylamine (18 mL, 128 mmol) was added. The ice bath was removed and the reaction quenched with saturated ammonium chloπde (75 mL) at 0°C, then extracted with ethyl acetate (3 x 150 mL). The combined ethyl acetate layer was washed with water (100 mL), bπne (100 mL), dned over anhydrous sodium sulfate, filtered, and concentrated. The residue was stirred in ethyl acetate and hexane, filtered to give 1-benzhydrylazetidin- 3-one (9 g, 33 mmol, 92%). To a stirring solution of the resulting 1-benzhydryl- azetidin-3-one (8.3 g, 30 mmol) in methylene chloride (120 mL) was added triethylamine (6.4 mL, 45 mmol), and carbethoxymethylene triphenylphosphorane (15.9 g, 45 mmol). This reaction mixture was stirred under argon at room temperature for 2 hrs., then quenched with acetone (1 mL), and concentrated in vacuo. The residue was stirred in 20% ethyl acetate in hexane (20 mL) as triphenyl phosphine oxide precipitated. The mixture was then filtered and concentrated. The crude product was chromatographed on silica gel, eluting with 50:40:10 methylene chloride: hexane: ether to give 9.3 g of the title compound. Step 3C: Azetidin-3-yl-acetic acid ethyl ester To a solution of (l-benzhydrylazetidin-3-ylidene)-acetic acid ethyl ester (5.0 g, 16 mmol) in ethanol (200 mL) was added palladium hydroxide on carbon (1.5 g). This mixture was vigorously shaken under a 50psi hydrogen atmosphere for 24 hrs, then filtered and concentrated to give an oil, used without additional purification. Step 3D: 3-Ethoxycarbonylmethyl-azetidine-l-carboxylic acid benzyl ester To a stirring mixture of azetidin-3-yl-acetic acid ethyl ester (1.0 g, 6.98 mmol), in saturated aqueous sodium bicarbonate (5 mL) and THF (5 mL) at 0°C was added carbobenzyloxy chloride (1.1 mL, 7.68 mmol) dropwise. The ice bath was removed and the reaction mixture was stirred at room temperature for 2 hrs, then extracted with ethyl acetate acetate (3 x 20 mL). The combined ethyl acetate layer was washed with water (10 mL), bnne (10 mL), dned over anhydrous sodium sulfate, filtered, and concentrated The residue was chromatographed on silica gel, eluting with 20% ethyl acetate in hexane to give 0 30 g of the title compound Step 3E 3-Carboxymethyl-azetιdιne-l-carboxylιc acid benzyl ester A solution of 3-ethoxycarbonylmethylazetιdιne-l-carboxylιc acid benzyl ester (0 30 g, 1 1 mmol), and lithium hydroxide monohydrate (0 091 g, 2 2 mmol) in THF (3 mL) and water (1 mL) was stirred under argon at room temperature for 1 hr After acidification with 2 M aqueous hydrochloπc acid (10 mL), the reaction mixture was extracted with ethyl acetate (3 x 20 mL) The combined ethyl acetate layers were washed with water (10 mL), bnne (10 mL), dned over anhydrous sodium sulfate, filtered, and concentrated to give 0 27 g of the title compound EXAMPLE 4 (S)-l-Phenyl-N-{4-r2-ammomethyl-3-methyl-5-(3-tπfluoromethyl- phenyl)-3H-ιmιdazol-4-yl1-pyndm-2-y] }-ethylamιne The compound was prepared as described in example 1, replacing 3- (benzyloxycarbonylamino)propanoic acid with N-Cbz-glycine in step IE. 'H NMR (300 MHz, CD 3 OD) d 8.01 (d, 7 = 5.4 Hz, 1 H, Pyr), 7.72 (s, 1 H, Ar), 7.52 (d, 7 = 7.6 Hz, 1 H, Ar), 7.47 (d, 7 = 7.8 Hz, 1 H, Ar), 7.37 (t, 7 = 7.6 Hz, 1 H, Ar), 7.32 - 7.22 (m, 4 H, Ar), 7.16 (t, 7 = 7.1 Hz, 1 H, Ar), 6.46 (dxd, 7 = 5.4 & 1.5 Hz, 1 H, Pyr), 6.35 (s, 1 H, Pyr), 4.82 (q, 7 = 6.8 Hz, 1 H, PhCHCH 3 ), 3.96 (s, 2 H, CCH 2 NH 2 ), 3.40 (s, 3 H, CH 3 N), 1.48 (d, 7 = 6.8 Ηz, 3 Η, PhCΗCH,); MS (FAB) Calcd for C 25 Η 24 F 3 N 5 (M + H + ) 451, found 452 EXAMPLE 5 (S)-l-Phenyl-N-|4-[2-(dimethylamino)methyl-3-methyl-5-(3-trifluoromethyl-phenyl)- 3H-imidazol-4-yll-pyridin-2-yl } -ethylamine The compound was prepared as described in example 1, replacing 3- (benzyloxycarbonylamino)propanoic acid with 3-carboxymethyl-azetidine-l- carboxylic acid benzyl ester in step IE. 'Η NMR (300 MHz, CD 3 OD) d 8.00 (d, 7 = 5.4 Hz, 1 H, Pyr), 7.71 (s, 1 H, Ar), 7.51 (d, 7 = 7.8 Hz, 1 H, Ar), 7.47 (d, 7 = 7.8 Hz, 1 H, Ar), 7.37 (t, 7 = 7.8 Hz, 1 H, Ar), 7.32 - 7.22 (m, 4 H, Ar), 7.16 (t, 7 = 7.1 Hz, 1 H, Ar), 6.46 (dxd, 7 = 5.4 & 1.5 Hz, 1 H, Pyr), 6.37 (s, 1 H, Pyr), 4.83 (q, 7 = 7.1 Hz, 1 H, PhCHCH 3 ), 3.61 (s, 2 H, CCH 2 N), 3.45 (s, 3 Η, CH 3 N), 2.31 (s, 6 Η, N(CH 3 ) 2 ), 1.48 (d, 7 = 6 8 Ηz, 3 Η, PhCΗCH 3 ), MS (FAB) Calcd for C 27 Η 2g F 3 N 5 (M + H + ) 479.2, found 480.3 EXAMPLE 6 (S)-l-Phenyl-N-{4-[2-(3-dιmethylamιno-propyl)-3-methyl-5-(3-tπfluoromethyl- phenyl)-3H-ιmιdazol-4-yll-pyπdιn-2-ylj-ethylamιne The compound was prepared as descnbed in example 1, replacing 3- (benzyloxycarbonylamιno)propanoιc acid with N-Cbz-GABA in step IE. Then a mixture of (S)-l-phenyl-N-{4-[2-(3-amιno-propyl)-3-methyl-5-(3-tπfluoromethyl- phenyl)-3H-ιmιdazol-4-yl]-pyndm-2-yl}-ethylamme (0.10 g, 0.21 mmol), formaldehyde (0.10 g, 1.25 mmol), and palladium on activated carbon (10%) (0.03 g) in methanol (5 mL) was vigorously stirred under 1 atm hydrogen for 6 hrs. The reaction mixture was then filtered, and concentrated. The residue was chromatographed on silica gel, elutmg with 90:10: 1 CΗ 2 Cl 2 :MeOΗ NΗ 4 OΗ to give 0.087 g of the title compound. 'H NMR (300 MHz, CD 3 OD) d 8.00 (d, 7 = 5.4 Hz, 1 H, Pyr), 7.68 (s, 1 H, Ar), 7.50 (d, 7 = 7.5 Hz, 1 H, Ar), 7.47 (d, 7 = 8.6 Hz, 1 H, Ar), 7.38 (t, 7 = 7.7 Hz, 1 H, Ar), 7.32 - 7.23 (m, 4 H, Ar), 7.16 (t, 7 = 6.9 Hz, 1 H, Ar), 6.46 (dxd, 7 = 5.4 & 1.5 Hz, 1 H, Pyr), 6.34 (s, 1 H, Pyr), 4.81 (q, 7 = 6.8 Hz, 1 H, PhCHCH 3 ), 3.34 (s, 3 H, CH 3 N), 2.83 (t, 7 = 7.7 Hz, 2 H, CH 2 CH 2 N), 2.55 (t, 7 = 7.7 Ηz, 2 Η, CCH 2 CΗ 2 ), 2.35 (s, 6 H, N(CH 3 )2), 1.97 (quin, 7 = 7.5 Ηz, 2 Η, CΗ 2 CH 2 CΗ 2 ), 1.48 (d, 7 = 6.8 Hz, 3 H, PhCHCH 3 ); MS (FAB) Calcd for C 29 Η 32 P 3 N 5 (M + H + ) 507.3, found 508.4 EXAMPLE 7 (S)-l-Phenyl-N-{4-[2-(3-amino-propyl)-3-methyl-5-(3-trifluoromethyl-phenyl)-3H- imidazol-4-yl1-pyridin-2-yl)-ethylamine The compound was prepared as described in example 1, replacing 3- (benzyloxycarbonylamino)propanoic acid with N-Cbz-GABA in step IE. Η NMR (300 MHz, CD 3 OD) d 8.00 (d, 7 = 5.1 Hz, 1 H, Pyr), 7.69 (s, 1 H, Ar), 7.49 (d, 7 = 7.6 Hz, 1 H, Ar), 7.47 (d, 7 = 5.9 Hz, 1 H, Ar), 7.37 (t, 7 = 7.8 Hz, 1 H, Ar), 7.32 - 7.22 (m, 4 H, Ar), 7.16 (t, 7 = 6.9 Hz, 1 H, Ar), 6.46 (dxd, 7 = 5.4 & 1.5 Hz, 1 H, Pyr), 6.34 (s, 1 H, Pyr), 4.81 (q, 7 = 6.8 Hz, 1 H, PhCHCH 3 ), 3.35 (s, 3 H, CH 3 N), 2.94 (t, 7 = 7.2 Ηz, 2 Η, CΗ 2 CH 2 NΗ 2 ), 2.89 (t, 7 = 7.3 Hz, 2 H, CCH 2 CH 2 ), 2.04 (quin, 7 = 7.3 Hz, 2 H, CH 2 CH 2 CH 2 ), 1.49 (d, 7 = 6.8 Hz, 3 H, PhCHCH 3 ); MS (FAB) Calcd for C 27 Η 2g F 3 N 5 (M + H + ) 479.2, found 480.3 EXAMPLE 8 j4-[3-methyl-2-(morpholin-4-ylmethyl)-5-(3-trifluoromethyl- phenyl)-3H-imidazol-4-yl1-pyridin-2-yl )-(l-phenylethyl)-amine The title compound was prepared as described in example 1, replacing 3-(benzyloxycarbonylamino)propanoic acid with morpholin-4-yl-acetic acid (from comercially available methyl moφholinoacetate by aqueous acid hydrolysis (6N ΗC1 reflux 2 h)) in step IE and omission of deprotection step 1Η. ] Η NMR (300 MHz, CD 3 OD) d 8.12 (d, 7 = 5.4 Hz, 1 H, Pyr), 7.79 (s, 1 H, Ar), 7.47 (d, 7 = 8.0 Hz, 1 H, Ar), 7.39 (d, 7 = 8.0 Hz, 1 H, Ar), 7.25 (m, 6 H, Ar), 6.47 (d, 7 = 5.4 Hz, 1 H, Pyr), 6.07 (s, 1 H, Pyr), 5.44 (d, 7 = 5.6, 1 H, NH), 4.62 (m, 1 H, PhCHCH 3 ), 3.70 (m, 4 H, CH.), 3.64 (s. 2 H, CH 2 ), 3.26 (s, 3 H, CH 3 ), 2.51 (m, 4 H, CH 2 ), 1.54 (d, 7 = 6.0 Hz, 3 H, PhCHCH 3 ). MS (FAB) Calcd for C 29 Η 30 F 3 N 5 O (M + H + ) 522.2, found 522.2 EXAMPLE 9 (S)-l-Phenyl-N-{4-r2-(3-methylaminopropyl)-3-methyl-5-(3- trifluoromethylphenyl)-3H-imidazol-4-yl]-pyridin-2-yl )-ethylamine The compound was prepared as described in example 7 and then methylated as shown in step ID. ! Η NMR (300 MHz, CD 3 OD) d 8.00 (d, 7 = 5.4 Hz, 1 H, Pyr), 7.69 (s, 1 H, Ar), 7.49 (d, 7 = 7.3 Hz, 1 H, Ar), 7.47 (d, 7 = 6.4 Hz, 1 H, Ar), 7.37 (t, 7 = 7.7 Hz, 1 H, Ar), 7.32 - 7.23 (m, 4 H, Ar), 7.16 (t, 7 = 6.9 Hz, 1 H, Ar), 6.46 (dxd, 7 = 5.4 & 1.5 Hz, 1 H, Pyr), 6.34 (s, 1 H, Pyr), 4.81 (q, 7 = 6.4 Hz, 1 H, PhCHCTL), 3.36 (s, 3 Η, CH 3 N), 2.87 (t, 7 = 7.5 Ηz, 2 Η, CΗ2CH 2 NΗ), 2.81 (t, 7 = 7.3 Hz, 2 H, CCH 2 CH 2 ), 2.49 (s, 3 H, NHCH 3 ), 2.00 (quin, 7 = 7.3 Ηz, 2 Η, CΗ 2 CH 2 CΗ 2 ), 1.48 (d, 7 = 6.8 Hz, 3 H, PhCHCH,); MS (FAB) Calcd for C 2g Η 30 F 3 N 5 (M + H + ) 493.2, found 494.3 EXAMPLE 10 (S)-l-Phenyl-N-{4-r2-(2-dιmethylamιnoethyl)-3-methyl-5-(3- tπfluoromethvIphenyl)-3H-ιmιdazol-4-yl]-pyndιn-2-yl 1-ethylamιne The compound was prepared as descnbed in example 1 then methylated as descnbed in example 6. Η NMR (300 MHz, CD 3 OD) d 8.00 (d, 7 = 5.1 Hz, 1 H, Pyr), 7.69 (s, 1 H, Ar), 7.50 (d, 7 = 7.6 Hz, 1 H, Ar), 7.46 (d, 7 = 7.8 Hz, 1 H, Ar), 7.36 (t, 7 = 7.7 Hz, 1 H, Ar), 7.31 - 7.22 (m, 4 H, Ar), 7.16 (t, 7 = 6.9 Hz, 1 H, Ar), 6.46 (dxd, 7 = 5.1 & 1.5 Hz, 1 H, Pyr), 6.34 (s, 1 H, Pyr), 4.81 (q, 7 = 6.8 Hz, 1 H, PhCHCH 3 ), 3.36 (s, 3 H, CH 3 N), 2.99 (t, 7 = 7.6 Ηz, 2 Η, CΗ 2 CH 2 N), 2.78 (t, 7 = 7.6 Ηz, 2 Η, CCH 2 CΗ 2 ), 2.37 (s, 6 H, N(CH 3 ) 2 ), 1.48 (d, 7 = 6.8 Ηz, 3 Η, PhCΗCH 3 ); MS (FAB) Calcd for C 2g Η 30 F 3 N 5 (M + H + ) 493.2, found 494.3 The ability of compounds of the present invention to inhibit the synthesis or the activity of cytokines can be demonstrated using the following in vitro assays. BIOLOGICAL ASSAYS Lipopolysaccharide mediated production of cytokines Human peripheral blood mononuclear cells (PBMC) are isolated from fresh human blood according to the procedure of Chin and Kostura, J. Immunol. 151, 5574-5585 (1993). Whole blood is collected by sterile venipuncture into 60 mL syringes coated with 1.0 mL of sodium- heparin (Upjohn, 1000 U/mL) and diluted 1: 1 in Hanks Balanced Salt Solution (Gibco). The erythrocytes are separated from the PBMC's by centrifugation on a Ficoll-Hypaque lymphocyte separation media. The PBMC's are washed three times in Hanks Balanced Salt Solution and then resuspended to a final concentration of 2 x 10 cell/mL in RPMI containing 10% fresh autologous human serum, penicillin streptomycin (10 U/mL) and 0.05% DMSO. Lipopolysaccharide (Salmonella type Re545; Sigma Chemicals) is added to the cells to a final concentration of 100 ng/mL. An aliquot (0.1 mL) of the cells is quickly dispensed into each well of a 96 well plate containing 0.1 mL of the test compound, at the appropriate dilution, and are incubated for 24 hours, at 37°C in 5% CO2 . At the end of the culture period, cell culture supernatants are assayed for IL-lβ , TNF-α, IL-6 and PGE2 production using specific ELISA. IL-1 mediated cytokine production Human peripheral blood mononuclear cells are isolated from fresh human blood according to the procedure of Chin and Kostura, I. Immunol. 151, 5574-5585 (1993). Whole blood is collected by sterile venipuncture into 60 mL syringes coated with 1.0 mL of sodium- heparin (Upjohn, 1000 U/mL) and diluted 1: 1 in Hanks Balanced Salt Solution (Gibco). The erythrocytes are separated from the PBMC's by centrifugation on a Ficoll-Hypaque lymphocyte separation media. The PBMC's are washed three times in Hanks Balanced Salt Solution and then resuspended to a final concentration of 2 x 10 cell/mL in RPMI containing 10% fresh autologous human serum, penicillin strepto-mycin (10 U/mL) and 0.05% DMSO. Endotoxin free recombinant human EL- lb is then added to a final concentration of 50 pMolar. An aliquot (0.1 mL) of the cells is quickly dispensed into each well of a 96 well plate containing 0.1 mL of the compound at the appropriate dilution, and are incubated for 24 hours, at 37°C in 5% CO2 . At the end of the culture period, cell culture supernatants are assayed for TNF-a, EL-6 and PGE2 synthesis using specific ELISA. Determination of EL-lβ , TNF- . IL-6 and Prostanoid Production from LPS or EL-l Stimulated PBMC's IL-lβ ELISA Human EL-lβ can be detected in cell-culture supernatants or whole blood with the following specific trapping ELISA. Ninety-six well plastic plates (Immulon 4; Dynatech) are coated for 12 hours at 4°C with 1 mg/mL protein-A affinity chromatography purified mouse anti-human EL-lβ monoclonal antibody (purchased as an ascites preparation from LAO Enteφrise, Gaithersburg Maryland.) diluted in Dulbecco's phosphate-buffered saline (-MgCl2, -CaCl2). The plates are washed with PBS-Tween (Kirkegaard and Perry) then blocked with 1% BSA diluent and blocking solution (Kirkegaard and Perry) for 60 minutes at room temperature followed by washing with PBS Tween. EL-lβ standards are prepared from purified recombinant EL-lβ produced from E. coll.. The highest concentration begins at 10 ng/mL followed by 11 two-fold serial dilutions. For detection of EL-l β from cell culture supernatants or blood plasma, 10 - 25 mL of supernatant is added to each test well with 75-90 mL of PBS Tween. Samples are incubated at room temperature for 2 hours then washed 6 times with PBS Tween on an automated plate washer (Dennly). Rabbit anti-human EL-lβ polyclonal antisera diluted 1:500 in PBS-Tween is added to the plate and incubated for 1 hour at room temperature followed by six washes with PBS-Tween. Detection of bound rabbit anti-EL-lβ IgG is accomplished with Fab' fragments of Goat anti-rabbit IgG-horseradish peroxidase conjugate (Accurate Scientific) diluted 1:10,000 in PBS-Tween. Peroxidase activity was determined using TMB peroxidase substrate kit (Kirkegaard and Perry) with quantitation of color intensity on a 96-well plate Molecular Devices spectrophotometer set to determine absorbance at 450 nM. Samples are evaluated using a standard curve of absorbance versus concentration. Four-parameter logistics analysis generally is used to fit data and obtain concentrations of unknown compounds. TNF-α ELISA Immulon 4 (Dynatech) 96-well plastic plates are coated with a 0.5 mg/mL solution of mouse anti-human TNF-a monoclonal antibody. The secondary antibody is a 1:2500 dilution of a rabbit anti-human TNF-α polyclonal serum purchased from Genzyme. All other operations are identical to those descnbed above for EL-lβ. The standards are prepared in PBS-Tween + 10% FBS or HS. Eleven 2 fold dilutions are made beginning at 20 ng/mL TNF-α. EL-6 ELISA Levels of secreted human EL-6 are also determined by specific trapping ELISA as descnbed previously in Chin and Kostura, J. Immunol. 151, 5574-5585 (1993). (Dynatech) ELISA plates are coated with mouse anti-human EL-6 monoclonal antibody diluted to 0.5 mg/mL in PBS. The secondary antibody, a rabbit anti-human EL-6 polyclonal antiserum, is diluted 1:5000 with PBS-Tween. All other operations are identical to those descnbed above for EL-lb. The standards are prepared in PBS-Tween + 10% FBS or HS. Eleven 2 fold dilutions are made beginning at 50 ng/mL EL-6. PGE? production Prostagland E2 is detected in cell culture supernatants from LPS or EL-l stimulated PBMC's using a commercially available enzyme immunoassay . The assay purchased from the Cayman Chemical (Catalogue number 514010) and is run exactly according to the manufacturers instructions. Interleukm8 (EL-8) The present compounds can also be assayed for EL-8 inhibitory activity as discussed below. Pnmary human umbilical cord endothehal cells (HUVEC) (Cell Systems, Kirland, Wa) are maintained in culture medium supplemented with 15% fetal bov e serum and 1% CS-HBGF consisting of aFGF and hepaπn. The cells are then diluted 20-fold before being plated (250 μl) into gelatin coated 96-well plates. Pπor to use, culture medium is replaced with fresh medium (200μl). Buffer or test compound (25 xl, at appropriate concentrations) is then added to each well in quadruplicate wells and the plates incubated for 6h in a humidified incubator at 37°C in an atmosphere of 5% CO2- At the end of the incubation period, supernatant is removed and assayed for EL-8 concentration using an EL-8 ELISA kit obtained from R&D Systems (Minneapolis, MN). All data is presented as mean value (ng/mL) of multiple samples based on the standard curve. IC50 values where appropriate are generated by non-linear regression analysis.

Claims: WHAT IS CLAIMED IS: A compound of the formula wherein X is C,-C 6 alkyl, said alkyl group optionally substituted by 1-3 groups selected from halogen, hydroxy, CF 3 , NH2 and NO2; or a heterocychc group connected to the imidazole ring by a direct bond or by C,-C 6 alkyl; R, and R 2 are independently hydrogen or C j -C 6 alkyl, said alkyl group optionally substituted by 1-3 groups selected from halogen, hydroxy, CF 3 , NH2 and NO2; or R, and R 2 taken together with the nitrogen atom represent an optionally substituted 4 to 10 membered non-aromatic heterocychc ring containing at least one N atom, and optionally containing 1-2 additional N atoms and 0-1 O or S atom; or R 2 and X taken together represent an optionally substituted 4 to 10 membered non- aromatic heterocychc ring containing at least one N atom, and optionally containing 1-2 additional N atoms and 0-1 O or S atoms; Q is CH or N; R 3 is hydrogen or NH(C 1 -C 6 alkyl)aryl; R., R, and Rg independently represent a member selected from the group consisting of hydrogen, halo, hydroxy, CF 3 NH 2 NO 2 C, -C 6 alkyl, said alkyl group optionally substituted by 1-3 groups selected from halogen, hydroxy, CF 3 , NΗ2 and NO2, ,-C 6 alkoxy, said alkoxy group optionally substituted by 1-3 groups selected from halogen, hydroxy, CF 3 , NH2 and NO2; 3 -C 8 cycloalkyl, said cycloalkyl group optionally substituted by 1-3 groups selected from halogen, hydroxy, CF 3 , NH) and NO2 or aryl, said aryl group optionally substituted by 1-3 groups selected from halogen, hydroxy, CF 3 , NH2 and NO2; R 7 is hydrogen or C,-C 6 alkyl, said alkyl group optionally substituted by 1-3 groups selected from halogen, hydroxy, CF 3 , NH2 and NO2; or a pharmaceutically acceptable addition salt and/or hydrate thereof, or where applicable, a geometric or optical isomer or racemic mixture thereof. 2. The compound in accordance with claim 1 of the formula or a pharmaceutically acceptable salt thereof, wherein: Q is CH; X is C1-C6 alkyl; or R 2 and X taken together represent an azetidine group; R Λ , RΓ and R^ are independently hydrogen or CF 3 ; R7 is CH3; R, and R2 are independently hydrogen or C,-Cg alkyl; or R, and R 2 taken together with the nitrogen atom form a moφholine group. 3. A compound of the formula: or a pharmaceutically acceptable addition salt and/or hydrate thereof, or where applicable, a geometric or optical isomer or racemic mixture thereof. 4. A pharmaceutical composition which is comprised of a compound in accordance with claim 1 in combination with a pharmaceutically acceptable carrier. 5. A pharmaceutical composition which is produced by combining a compound in accordance with claim 1 and a pharmaceutically acceptable carrier. 6. A method of treating a cytokine mediated disease in a mammal, comprising administering to a mammalian patient in need of such treatment an amount of a compound as described in claim 1 in an amount which is effective to treat said cytokine mediated disease. 7. A method of treating inflammation in a mammalian patient in need of such treatment, which is comprised of administering to said patient an anti- inflammatory effective amount of a compound as described in claim 1. 8. A method in accordance with claim 6 wherein the cytokine mediated disease is rheumatoid arthritis, osteoaithritis, endotoxemia, toxic shock syndrome, inflammatory bowel disease, tuberculosis, atherosclerosis, muscle degeneration, cachexia, psoriatic arthritis, Reiter's syndrome, rheumatoid arthritis, gout, traumatic arthritis, rubella arthritis or acute synovitis. 9. A method in accordance with claim 6 wherein the cytokine mediated disease is rheumatoid arthritis, rheumatoid spondylitis, osteoarthritis, gouty arthritis, sepsis, septic shock, endotoxic shock, gram negative sepsis, toxic shock syndrome, adult respiratory distress syndrome, cerebral malaria, chronic pulmonary inflammatory disease, silicosis, pulmonary sarcosis, bone resoφtion diseases, reperfusion injury, graft v. host rejection, allograft rejection, fever, myalgia due to infection, cachexia secondary to infection or malignancy, cachexia secondary to acquired immune deficiency syndrome (AEDS), AEDS related complex (ARC), keloid formation, scar tissue formation, Crohn's disease, ulcerative colitis or pyresis. 10. A method of treating osteoporosis in a mammalian patient in need of such treatment, which is comprised of administering to said patient an amount of a compound as described in claim 1 which is effective to treat osteoporosis. 11. A method of treating bone resoφtion in a mammalian patient in need of such treatment, which is comprised of administering to said patient an amount of a compound as described in claim 1 which is effective to treat bone resoφtion. 12. A method of treating Crohn's disease in a mammalian patient in need of such treatment which is comprised of administering to said patient an amount of a compound as described in claim 1 which is effective to treat Crohn's disease. 13. A process for making a pharmaceutical composition comprising combining a compound of Claim 1 and a pharmaceutically acceptable carrier.

Inventors: CLAIBORNE CHRISTOPHER F, CLAREMON DAVID A, LIVERTON NIGEL J, NGUYEN KEVIN T, CLAIBORNE, CHRISTOPHER, F., CLAREMON, DAVID, A., LIVERTON, NIGEL, J., NGUYEN, KEVIN, T.
Applicants: CLAIBORNE CHRISTOPHER F, CLAREMON DAVID A, LIVERTON NIGEL J, MERCK & CO INC, NGUYEN KEVIN T, CLAIBORNE, CHRISTOPHER, F., CLAREMON, DAVID, A., LIVERTON, NIGEL, J., MERCK & CO., INC., NGUYEN, KEVIN, T.
Application year: 2000
Main classification: A61K31
IPC codes: A61K31, A61P1, A61P9, A61P11, A61P17, A61P19, A61P21, A61P29, A61P31, A61P35, A61P37, A61P39, C07D401, C07D403