SODIUM-HYDROGEN EXCHANGER TYPE 1 INHIBITOR CRYSTALS

Abstract: NHE-1 inhibitor crystal of Formula (I), methods of using such NHE-1 inhibitor crystal and pharmaceutical compositions containing such NHE-1 inhibitor crystal. The NHE-1 inhibitor crystal is useful for the reduction of tissue damage resulting from tissue ischemia.

Description: SODIUM-HYDROGEN EXCHANGER TYPE 1 INHIBITOR CRYSTALS BACKGROUND OF INVENTION This invention relates to sodium-hydrogen exchanger type 1 (NHE-1) inhibitors and particularly, crystals of such inhibitors Myocardial ischemic injury can occur in out-patient as well as in penoperative settings and can lead to the development of sudden death, myocardial infarction or congestive heart failure There is an unmet medical need to prevent or minimize myocardial ischemic injury, particularly penoperative myocardial infarction Such a therapy is anticipated to be life-saving and reduce hospitalizations, enhance quality of life and reduce overall health care costs of high risk patients Pharmacological cardioprotection would reduce the incidence and progression of myocardial infarction and dysfunction occurring in these surgical settings (penoperatively) In addition to reducing myocardial damage and improving post-ischemic myocardial function in patients with ischemic heart disease, cardioprotection would also decrease the incidence of cardiac morbidity and mortality due to myocardial infarction and dysfunction in patients "at risk" (such as greater than 65 years, exercise intolerant, coronary artery disease, diabetes mellitus, hypertension) that require non-cardiac surgery The mechanιsm(s) responsible for the myocardial injury observed after ischemia and reperfusion is not fully understood A variety of publications have disclosed the use of guanidine derivatives as useful for the treatment of, for example, arrhythmias A recent published patent application, PCT/IB99/00206 published as WO 99/43663 on September 2, 1999, the disclosure of which is hereby incorporated by reference, discloses a variety of NHE-1 inhibitors including [5-cyclopropyl-1- (quιnolιn-5-yl)-1 H-pyrazole-4-carbonyl]guanιdιne The publication further states that "preferred salts of the immediately preceding compound are the mono- or di- mesylate salts " PCT/JP97/04650 application published on June 25, 1998 discloses N- [(substituted five-membered heteroaryl)]guanιdιne compounds which are disclosed to be useful as inhibitors of Na + /H + exchange and consequently effective for the treatment of various diseases such as hypertension, arrhythmia, angina pectons, myocardial infarct, arteriosclerosis, and complications of diabetes Thus, there is clearly a need and a continuing search in this field of art for compounds for the treatment of penoperative myocardial ischemia and, accordingly, new crystal forms of such compounds SUMMARY OF THE INVENTION This invention is directed to a crystal of Formula I Alternatively, the above salt is named as N-(5-cyclopropyl-1-quιnolιn- 5-yl-1 H-pyrazole-4-carbonyl)-guanιdιne, monomesylate salt Another aspect of this invention is directed to an anhydrous crystal form of the Formula I salt Another aspect of this invention is directed to an anhydrous crystal form A of the Formula I salt having the X-ray diffraction d-spacing of Table III below Another aspect of this invention is directed to an anhydrous crystal form A of the Formula I salt having the X-ray powder diffraction pattern as shown in Figure 1 Another aspect of this invention is directed to an anhydrous crystal form D of the Formula I salt having the X-ray diffraction d-spacing of Table II below Another aspect of this invention is directed to an anhydrous crystal form D of the Formula I salt having the X-ray powder diffraction pattern as shown in Figure Another aspect of this invention is directed to a hemihydrate crystal form of the Formula I salt preferably having the X-ray diffraction d-spacing of Table IV below Another aspect of this invention is directed to a hemihydrate crystal form of the Formula I salt having the X-ray powder diffraction pattern as shown in Figure 3 In the text herein, including the following methods, pharmaceutical compositions, combinations and kits, reference is made to a crystal of Formula I While it is understood that if the crystal is in solution, the crystal form is not present (in contrast to e g , a dry tablet formulation), the following methods, pharmaceutical compositions, combinations and kits are intended to include a method or formulation resulting from such crystal (e g , administering an I V solution made from the crystal) Another aspect of this invention is a method of treating a mammal (e g , human) having a disease or condition mediated by NHE-1 by administering a therapeutically effective amount of a crystal of Formula I to the mammal Another aspect of this invention is directed to a method of reducing tissue damage (e g , substantially preventing tissue damage, inducing tissue protection) resulting from ischemia comprising administering to a mammal (e g , a female or male human) in need of such treatment a therapeutically effective amount of a crystal of Formula I Preferred ischemic tissues taken individually or as a group are wherein the ischemic tissue is cardiac, brain, liver, kidney, lung, gut, skeletal muscle, spleen, pancreas, nerve, spinal cord, retina tissue, the vasculature, or intestinal tissue An especially preferred ischemic tissue is cardiac tissue It is especially preferred that the crystals are administered to prevent penoperative myocardial ischemic injury Preferably, the crystals of this invention are administered prophylactically The ischemic damage may occur during organ transplantation either to the organ or the patient Preferably, the crystals of this invention are administered prior to, during and/or shortly after, cardiac surgery or non-cardiac surgery In one aspect of this invention a crystal of Formula I is administered locally A preferred dosage is about 0 001 to 100 mg/kg/day of a Formula I crystal An especially preferred dosage is about 0 01 to 50 mg/kg/day of a Formula I crystals Another aspect of this invention is directed to a method of reducing myocardial tissue damage (e g , substantially preventing tissue damage, inducing tissue protection) during surgery (e g , coronary artery bypass grafting (CABG) surgeries, vascular surgeries, percutaneous transluminal coronary angioplasty (PTCA), organ transplantation, or other non-cardiac surgeries) comprising administering to a mammal (e g , a female or male human) a therapeutically effective amount of a crystal of Formula I Another aspect of this invention is directed to a method of reducing myocardial tissue damage (e g , substantially preventing tissue damage, inducing tissue protection) in patients presenting with ongoing cardiac (acute coronary syndromes, e g myocardial infarction or unstable angina) or cerebral ischemic events (e g , stroke) comprising administering to a mammal (e g , a female or male human) a therapeutically effective amount of a crystal of Formula I Another aspect of this invention is directed to a chronic method of reducing myocardial tissue damage (e g , substantially preventing tissue damage, inducing tissue protection) in a patient with diagnosed coronary heart disease (e g , previous myocardial infarction or unstable angina) or patients who are at high risk for myocardial infarction (e g , age > 65 and two or more risk factors for coronary heart disease) comprising administering to a mammal (e g , a female or male human) a therapeutically effective amount of a crystal of Formula I Another aspect of this invention is directed to a method of preventing ischemic damage comprising the chronic oral administration to a mammal in need of such treatment a therapeutically effective amount of a crystal of Formula I Another aspect of this invention is directed to a method for treating cardiovascular diseases comprising administering to a mammal (e g , a female or male human) a therapeutically effective amount of a crystal of Formula I Another aspect of this invention is directed to a method for treating arteriosclerosis comprising administering to a mammal (e g , a female or male human) a therapeutically effective amount of a crystal of Formula I Another aspect of this invention is directed to a method for treating hypertension comprising administering to a mammal (e g , a female or male human) a therapeutically effective amount of a crystal of Formula I Another aspect of this invention is directed to a method for treating arrhythmia comprising administering to a mammal (e g , a female or male human) a therapeutically effective amount of a crystal of Formula I Another aspect of this invention is directed to a method for treating angina pectons comprising administering to a mammal (e g , a female or male human) a therapeutically effective amount of a crystal of Formula I Another aspect of this invention is directed to a method for treating cardiac hypertrophy comprising administering to a mammal (e g , a female or male human) a therapeutically effective amount of a crystal of Formula I Another aspect of this invention is directed to a method for treating renal diseases comprising administering to a mammal (e g , a female or male human) a therapeutically effective amount of a crystal of Formula I Another aspect of this invention is directed to a method for treating diabetic complications comprising administering to a mammal (e g , a female or male human) a therapeutically effective amount of a crystal of Formula I Another aspect of this invention is directed to a method for treating restenosis comprising administering to a mammal (e g , a female or male human) a therapeutically effective amount of a crystal of Formula I Another aspect of this invention is directed to a method for treating diseases of cell proliferation comprising administering to a mammal (e g , a female or male human) a therapeutically effective amount of a crystal of Formula I Another aspect of this invention is directed to a method for treating cancerous diseases comprising administering to a mammal (e g , a female or male human) a therapeutically effective amount of a crystal of Formula I Another aspect of this invention is directed to a method for treating fibrotic diseases comprising administering to a mammal (e g , a female or male human) a therapeutically effective amount of a crystal of Formula I Another aspect of this invention is directed to a method for treating glomerular nephrosclerosis comprising administering to a mammal (e g , a female or male human) a therapeutically effective amount of a crystal of Formula I Another aspect of this invention is directed to a method for treating organ hypertrophies or hyperplasias comprising administering to a mammal (e g , a female or male human) a therapeutically effective amount of a crystal of Formula I Another aspect of this invention is directed to a method for treating pulmonary fibrosis comprising administering to a mammal (e g , a female or male human) a therapeutically effective amount of a crystal of Formula I Another aspect of this invention is directed to a method for treating cerebro ischemic disorders comprising administering to a mammal (e g , a female or male human) a therapeutically effective amount of a crystal of Formula I Another aspect of this invention is directed to a method for treating myocardial stunning comprising administering to a mammal (e g , a female or male human) a therapeutically effective amount of a crystal of Formula I Another aspect of this invention is directed to a method for treating myocardial dysfunction comprising administering to a mammal (e g , a female or male human) a therapeutically effective amount of a crystal of Formula I Another aspect of this invention is directed to a method for treating cerebrovascular diseases comprising administering to a mammal (e g , a female or male human) a therapeutically effective amount of a crystal of Formula I Another aspect of this invention is directed to a method for treating organ hypertrophies or hyperplasias comprising administering to a mammal (e g , a female or male human) a therapeutically effective amount of a crystal of Formula I This invention is also directed to pharmaceutical compositions which comprise a therapeutically effective amount of a crystal of Formula I and a pharmaceutically acceptable carrier, vehicle or diluent This invention is also directed to pharmaceutical compositions for the reduction of tissue damage resulting from ischemia which comprise a therapeutically effective amount of a crystal of Formula I and a pharmaceutically acceptable carrier vehicle or diluent Yet another aspect of this invention are combinations of a crystal of Formula I and other compounds as described below Yet another aspect of this invention is directed to therapeutically effective amounts of a pharmaceutical composition comprising a crystal of Formula I and a cardiovascular agent and for the use of such a composition for the reduction of tissue damage resulting from tissue ischemia in mammals (e g , humans, male or female) Another aspect of this invention is a method of reducing tissue damage (e g , substantially preventing tissue damage, inducing tissue protection) resulting from or which could result from ischemia comprising administering to a mammal (e g , a female or male human) a a crystal of Formula I, and b a cardiovascular agent wherein the amounts of the first and second compounds result in a therapeutic effect Another aspect of this invention is a kit comprising a a crystal of Formula I and a pharmaceutically acceptable carrier, vehicle or diluent in a first unit dosage form, b a cardiovascular agent and a pharmaceutically acceptable carrier, vehicle or diluent in a second unit dosage form, and c means for containing said first and second dosage forms wherein the amounts of the first and second compounds result in a therapeutic effect In the above combination compositions, combination methods and kits, preferably the cardiovascular agents are, for example, β-blockers (e g , acebutolol, atenolol, bopindolol, labetolol, mepindolol, nadolol, oxprenol, pindolol, propranolol, sotalol), calcium channel blockers (e g , amlodipine, nifedipine, nisoldipme, nitrendipine, verapamil), potassium channel openers, adenosme adenosine agonists, ACE inhibitors (e g , captopπl, enalapπl), nitrates (e g , isosorbide dinitrate, isosorbide 5-mononιtrate, glyceryl tnnitrate), diuretics (e g , hydrochlorothiazide, indapamide, piretanide, xipamide), glycosides (e g , digoxin, metildigoxin), thrombolytics (e g , tPA), platelet inhibitors (e g , reopro), aspirin, dipyridamol, potassium chloride, clonidine, prazosin or adenosine A 3 receptor agonists This invention is also directed to a pharmaceutical combination composition comprising a therapeutically effective amount of a composition comprising a first compound, said first compound being a crystal of Formula I, a second compound, said second compound being a glycogen phosphorylase inhibitor, and/or optionally a pharmaceutical carrier, vehicle or diluent Another aspect of this invention is a method of reducing tissue damage (e g , substantially preventing tissue damage, inducing tissue protection) resulting from or which could result from ischemia comprising administering to a mammal (e g , a female or male human) a a first compound, said first compound being a crystal of Formula I, and b a second compound, said second compound being a glycogen phosphorylase inhibitor, wherein the amounts of the first and second compounds result in a therapeutic effect Another aspect of this invention is a kit comprising a a crystal of Formula I and a pharmaceutically acceptable carrier, vehicle or diluent in a first unit dosage form, b a glycogen phosphorylase inhibitor and a pharmaceutically acceptable carrier, vehicle or diluent in a second unit dosage form, and c means for containing said first and second dosage forms wherein the amounts of the first and second compounds result in a therapeutic effect In the above combination compositions, combination methods and kits preferred glycogen phosphorylase inhibitors are 5-chloro-1 H-ιndole-2-carboxylιc acid [(1 S)-((R)-hydroxy- dιmethylcarbamoyl-methyl)-2-phenyl-ethyl]-amιde, 5,6-dιchloro-1 H-ιndole-2-carboxylιc acid {(1 S)-[(R)-hydroxy-(methoxy- methyl-carbamoyl)-methyl]-2-phenyl-ethyl}-amιde, 5-chloro-1 H-ιndole-2-carboxylιc acid {(1 S)-[(R)-hydroxy-(methoxy-methyl- carbamoyl)-methyl]-2-phenyl-ethyl}-amιde, 5-chloro-1 H-ιndole-2-carboxylιc acid ((1 S)-{(R)-hydroxy-[(2-hydroxy- ethyl)-methyl-carbamoyl]-methyl}-2-phenyl-ethyl)-amιde 5-chloro-1 H-ιndole-2-carboxylιc acid {(1 S)-[(R)-hydroxy-(methyl-pyπdιn-2- yl-carbamoyl)-methyl]-2-phenyl-ethyl}-amιde 5-chloro-1 H-ιndole-2-carboxylιc acid ((1 S)-{(R)-hydroxy-[methyl-(2- pyrιdιn-2-yl-ethyl)-carbamoyl]-methyl}-2-phenyl-ethyl)-amιde, 5-chloro-1 H-ιndole-2-carboxylιc acid [(1 S)-benzyl-(2R)-hydroxy-3-(4- methyl-pιperazιn-1-yl)-3-oxo-propyl]-amιde hydrochloπde, 5-chloro-1 H-ιndole-2-carboxylιc acid [(1 S)-benzyl-(2R)-hydroxy-3-(3- hydroxy-azetιdιn-1-yl)-3-oxo-propyl]-amιde, 5-chloro-1 H-ιndole-2-carboxylιc acid ((1 S)-benzyl-(2R)-hydroxy-3- ιsoxazolιdιn-2-yl-3-oxo-propyl)-amιde, 5-chloro-1 H-ιndole-2-carboxylιc acid ((1 S)-benzyl-(2R)-hydroxy-3- [1 ,2]oxazιnan-2-yl-3-oxo-propyl)-amιde, 5-chloro-1 H-ιndole-2-carboxylιc acid [(1 S)-benzyl-(2R)-hydroxy-3-((3S)- hydroxy-pyrrolιdιn-1-yl)-3-oxo-propyl]-amιde, 5-chloro-1 H-ιndole-2-carboxylιc acid [(1S)-benzyl-3-((3S,4S)-dιhydroxy- pyrrolιdιn-1-yl)-(2R)-hydroxy-3-oxo-propyl]-amιde, 5-chloro-1 H-ιndole-2-carboxylιc acid [(1 S)-benzyl-3-((3R,4S)-dιhydroxy- pyrrolιdιn-1-yl)-(2R)-hydroxy-3-oxo-propyl]-amιde, 5-chloro-1 H-ιndole-2-carboxylιc acid ((1 S)-benzyl-(2R)-hydroxy-3- morpholιn-4-yl-3-oxo-propyl)-amιde, 5-chloro-1 H-ιndole-2-carboxylιc acid [(1 S)-benzyl-2-(3-hydroxyιmιno- pyrrolιdιn-1-yl)-2-oxo-ethyl]-amιde, 5-chloro-1 H-ιndole-2-carboxylιc acid [2-(cιs-3,4-dιhydroxy-pyrrolιdιn-1 -yl)- 2-oxo-ethyl]-amιde, 5-chloro-1 H-ιndole-2-carboxylιc acid [2-((3S,4S)-dιhydroxy-pyrrolιdιn-1 - yl)-2-oxo-ethyl]-amιde, 5-chloro-1 H-ιndole-2-carboxylιc acid [(1 S)-benzyl-2-(cιs-3,4-dιhydroxy- pyrrolιdιn-1-yl)-2-oxo-ethyl]-amιde, 5-chloro-1 H-ιndole-2-carboxylιc acid [2-(1 ,1-dιoxo-thιazolιdιn-3-yl)-2-oxo- ethyl]-amιde, 5-chloro-1 H-ιndole-2-carboxylιc acid (2-oxo-2-thιazolιdιn-3-yl-ethyl)- amide, 5-chloro-1 H-ιndole-2-carboxylιc acid [(1S)-(4-fluoro-benzyl)-2-(4-hydroxy- pιperιdιn-1-yl)-2-oxo-ethyl]-amιde, 5-chloro-1 H-ιndole-2-carboxylιc acid [(1 S)-benzyl-2-((3RS)-hydroxy- pιperιdιn-1 -yl)-2-oxo-ethyl]-amιde, 5-chloro-1 H-ιndole-2-carboxylιc acid [2-oxo-2-((1 RS)-oxo-1-thιazolιdιn-3- yl)-ethyl]-amιde, 5-chloro-1 H-ιndole-2-carboxylιc acid [(1 S)-(2-fluoro-benzyl)-2-(4-hydroxy- pιperιdιn-1-yl)-2-oxo-ethyl]-amιde, 5-chloro-1 H-ιndole-2-carboxylιc acid [(1 S)-benzyl-2-((3S,4S)-dιhydroxy- pyrrolιdιn-1-yl)-2-oxo-ethyl]-amιde, 5-chloro-1 H-ιndole-2-carboxylιc acid [(1 S)-benzyl-2-(3-hydroxy-azetιdιn-1 ■ yl)-2-oxo-ethyl]-amιde, 5-chloro-1 H-ιndole-2-carboxylιc acid [(1 S)-benzyl-2-(3-hydroxyιmιno- azetιdιn-1 -yl)-2-oxo-ethyl]-amιde and 5-chloro-1 H-ιndole-2-carboxylιc acid [(1 S)-benzyl-2-(4-hydroxyιmιno- pιperιdιn-1-yl)-2-oxo-ethyl]-amιde This invention is also directed to a pharmaceutical combination composition comprising a therapeutically effective amount of a composition comprising a first compound, said first compound being a crystal of Formula I, a second compound, said second compound being an aldose reductase inhibitor, and/or optionally a pharmaceutical carrier, vehicle or diluent Another aspect of this invention is a method of reducing tissue damage (e g , substantially preventing tissue damage, inducing tissue protection) resulting from or which could result from ischemia comprising administering to a mammal (e g , a female or male human) a a first compound, said first compound being a crystal of Formula I, and b a second compound, said second compound being an aldose reductase inhibitor, wherein the amounts of the first and second compounds result in a therapeutic effect Another aspect of this invention is a kit comprising a a crystal of Formula I and a pharmaceutically acceptable carrier, vehicle or diluent in a first unit dosage form, b an aldose reductase inhibitor and a pharmaceutically acceptable carrier, vehicle or diluent in a second unit dosage form, and c means for containing said first and second dosage forms wherein the amounts of the first and second compounds result in a therapeutic effect In the above combination compositions, combination methods and kits a preferred aldose reductase inhibitor is zopolrestat 1-phthalazιneacetιc acid, 3,4- dιhydro-4-oxo-3-[[5-trιfluoromethyl)-2-benzothιazolyl]methyl]- This invention is also directed to pharmaceutical compositions which comprise a therapeutically effective amount of a crystal of Formula I and a pharmaceutically acceptable carrier, diluent or excipient This invention is also directed to pharmaceutical compositions for the reduction of tissue damage resulting from ischemia which comprise a therapeutically effective amount of a crystal of Formula I and a pharmaceutically acceptable carrier, diluent or excipient The term "reduction" is intended to include partial prevention or prevention which, although greater than that which would result from taking no compound or from taking a placebo, is less than 100% in addition to substantially total prevention The term "damage resulting from ischemia" as employed herein refers to conditions directly associated with reduced blood flow to tissue, for example due to a clot or obstruction of blood vessels which supply blood to the subject tissue and which result, inter a a, in lowered oxygen transport to such tissue, impaired tissue performance, tissue dysfunction and/or necrosis Alternatively, where blood flow or organ perfusion may be quantitatively adequate, the oxygen carrying capacity of the blood or organ perfusion medium may be reduced, e g , in hypoxic environment, such that oxygen supply to the tissue is lowered, and impaired tissue performance, tissue dysfunction, and/or tissue necrosis ensues The term "treating", "treat" or "treatment" as used herein includes preventative (e g , prophylactic) and palliative treatment By "pharmaceutically acceptable" it is meant the carrier, diluent, excipients, and/or salt must be compatible with the other ingredients of the formulation, and not deleterious to the recipient thereof As used herein, the expressions "reaction-inert solvent" and "inert solvent" refers to a solvent or mixture of solvents which does not interact with starting materials, reagents, intermediates or products in a manner which adversely affects the yield of the desired product It will be recognized that the compound of this invention can exist in radiolabelled form, i e , the compound may contain one or more atoms containing an atomic mass or mass number different from the atomic mass or mass number ordinarily found in nature Radioisotopes of hydrogen, carbon, phosphorous, fluorine and chlorine include 3 H, 1 C, 32 P, 35 S, 18 F and 36 CI, respectively A compound of this invention, which contains those radioisotopes and/or other radioisotopes of other atoms are within the scope of this invention Tπtiated, i e , 3 H, and carbon-14, i e , 14 C, radioisotopes are particularly preferred for their ease of preparation and detectability A radiolabelled Formula I compound can generally be prepared by methods well known to those skilled in the art Conveniently, such radiolabelled compounds can be prepared by carrying out the procedures disclosed in the Schemes and/or in the Examples below by substituting a readily available radiolabelled reagent for a non-radiolabelled reagent Other features and advantages will be apparent from the specification and claims which describe the invention Brief Description of the Drawings FIG 1 is a characteristic x-ray powder diffraction pattern showing that Form A anhydrous N-(5-cyclopropyl-1 -quιnolιn-5-yl-1 H-pyrazole-4-carbonyl)-guanιdιne, monomesylate salt is crystalline (Vertical Axis Intensity (CPS), Horizontal Axis Two theta (degrees)) FIG 2 is a characteristic x-ray powder diffraction pattern showing that Form D anhydrous N-(5-cyclopropyl-1-quιnolιn-5-yl-1 H-pyrazole-4-carbonyl)-guanιdιne, monomesylate salt is crystalline (Vertical Axis Intensity (CPS), Horizontal Axis Two theta (degrees)) FIG 3 is the characteristic x-ray powder diffraction pattern of the Form C, hemihydrate N-(5-cyclopropyl-1 -quιnolιn-5-yl-1 H-pyrazole-4-carbonyl)-guanιdιne, monomesylate salt (Vertical Axis Intensity (CPS), Horizontal Axis Two theta (degrees)) DETAILED DESCRIPTION OF THE INVENTION In general the crystals of this invention can be made by processes which include processes analogous to those known in the chemical arts, particularly in light of the description contained herein Certain processes for the manufacture of the crystals of this invention are provided as further features of the invention and are illustrated by the following reaction scheme Other processes are described in the experimental section SCHEME I According to Scheme I the Formula II compound is combined with excess Formula III compound, (N,N-dιmethyl amide dimethyl acetal), optionally, in the presence of an acid catalyst such as p-toluenesulfonic acid, under neat conditions at a temperature of about 50°C to about 110°C for about one to about five hours, preferably at a temperature of about 70°C to about 80°C for about one to about two hours to prepare the Formula IV compound This reaction can be performed in ethyl acetate as well The Formula IV compound is cyclized with a Formula V compound in an inert solvent such as ethanol, preferably in the presence of an amine base such as triethylamine at a temperature of about 50°C to about reflux (78°C) for about 1 hour to about four hours to form the Formula VI pyrazole compound This reaction may also be performed in ethyl acetate and methanol The Formula VI pyrazole is hydrolyzed with a base such as sodium hydroxide in a solvent such as methanol conveniently at ambient temperature or preferably at elevated temperature (e g , reflux) for about one hour to about five hours to prepare the Formula VII acid Generally, the Formula VII acid is coupled with guanidine in the presence of a suitable coupling agent A suitable coupling agent is one which transforms a carboxylic acid into a reactive species which forms an amide linkage on reaction with an amine The coupling agent may be a reagent which effects this condensation in a one pot process when mixed together with the carboxylic acid and guanidine Exemplary coupling reagents are 1-(3-dιmethylamιnopropyl)-3-ethylcarbodιιmιde hydrochlonde-hydroxybenzotriazole (EDC/HOBT), dιcyclohexylcarbodιιmιde/hydroxybenzotrιazole(HOBT), 2-ethoxy-1-ethoxycarbonyl- 1 ,2-dιhydroquιnolιne (EEDQ), and diethylphosphorylcyanide The coupling is performed in an inert solvent, preferably an aprotic solvent at a temperature of about -20°C to about 50°C for about 1 to about 48 hours, in the presence of excess guanidine as base Exemplary solvents include acetonitnle, dichloromethane, dimethylformamide and chloroform or mixtures thereof The coupling agent can be an agent which converts the carboxylic acid to an activated intermediate which is isolated and/or formed in a first step and allowed to react with guanidine in a second step Examples of such coupling agents and activated intermediates are thionyl chloride or oxalyl chloride to form the acid chloride, cyanu c fluoride to form an acid fluoride or an alkyl chloroformate such as isobutyl or isopropenyl chloroformate or propanephosphonic anhydride (propanephosphonic acid anhydride, PPA) (with a tertiary amine base) to form a mixed anhydride of the carboxylic acid, or carbonyldnmidazole to form an acylimidazole If the coupling agent is oxalyl chloride, it is advantageous to employ a small amount of dimethylformamide as cosolvent with another solvent (such as dichloromethane) to catalyze the formation of the acid chloride This activated acid derivative may be coupled by mixing with the intermediate in an appropriate solvent together with an appropriate base Appropriate solvent/base combinations are, for example, dichloromethane, dimethylformamide or acetonitnle or mixtures thereof in the presence of excess guanidine as base Other appropriate solvent/base combinations include water or a ((Cι-C 5 )alcohol) or a mixture thereof together with a cosolvent such as dichloromethane, tetrahydrofuran or dioxane and a base such as sodium potassium or lithium hydroxide in sufficient quantity to consume the acid liberated in the reaction Use of these coupling agents and appropriate selection of solvents and temperatures are known to those skilled in the art or can be readily determined from the literature These and other exemplary conditions useful for coupling carboxylic acids are described in Houben-Weyl, Vol XV, part II, E Wunsch, Ed , G Theime Verlag, 1974, Stuttgart, M Bodansky, Principles of Peptide Synthesis, Springer- Verlag, Berlin 1984, and The Peptides, Analysis, Synthesis and Biology (ed E Gross and J Meienhofer), vols 1-5 (Academic Press, NY 1979-1983) In a preferred embodiment, the Formula VII acid is activated with an excess of thionyl chloride (e g , 3 to 6 equivalents) in an aprotic solvent such as toluene at a temperature of about 60°C to about 90°C for about fifteen minutes to about two hours, preferably at a temperature of about 75°C for about one to two hours The resulting Formula VIII activated acid chloride in anhydrous tetrahydrofuran is combined with excess guanidine hydrochloπde and an aqueous solution of an inorganic base (e g , sodium hydroxide) in anhydrous tetrahydrofuran at a temperature of about -20°C to about 10°C for about one hour to about three hours with warming to ambient temperature over the last hour to prepare the Formula IX compound The Formula IX compound is combined with methanesulfonic acid in an aprotic solvent, preferably a mixture of acetone and 1-methyl-2-pyrrolιdιnone, preferably about 90% to about 60% acetone, the remainder 1-methyl-2- pyrrolidinone, at a temperature of about 40°C to about 80°C for about 10 minutes to about one hour followed by stirring at a temperature of about 20°C to about 30°C for about 3 hours to about 6 hours, preferably at a temperature of about ambient for about 5 hours in the absence of light Preferably the solids are reslurned in acetone for about 6 to about 18 hours The salt formation can also be performed in tetrahydrofuran With this choice of solvent, a 95% ethanol reslurry is preferred The starting materials and reagents for the above described compounds, are also readily available or can be easily synthesized by those skilled in the art using conventional methods of organic synthesis For example, many of the compounds used herein are related to, or are derived from compounds found in nature, in which there is a large scientific interest and commercial need, and accordingly many such compounds are commercially available or are reported in the literature or are easily prepared from other commonly available substances by methods which are reported in the literature An anhydrous crystalline form (D) of the compound of this invention may be prepared by combining an acetone solution of N-(5-cyclopropyl-1-quιnolιn-5-yl-1 H- pyrazole-4-carbonyl)-guanιdιne and N-methyl pyrrolidinone with methanesulfonic acid at a temperature of about 30°C to about 60°C, preferably about 50°, for about 1 to about 10 hours, typically followed by agitation with cooling to a temperature of about 5°C, preferably less than 10°C Alternative solvents include organic solvents such as methanol, ethanol and isopropanol As appropriate, the product is filtered and dried under vacuum at 55°C-60°C for 24 to 72 hours until the solvent content is less than or equal to 0 5% by gas chromatograph An anhydrous crystalline form (A) of the compound of this invention may be prepared from the above Form D crystalline form by repulping (i e , mixing a suspension of partially dissolved material) from acetone at a temperature of about 20°C to about 25°C, preferably ambient temperature, with agitation, for about 2 hours to about 24 hours, typically followed by drying at a temperature of about 30°C to a temperature of about 60°C Alternative solvents include inert solvents such as acetonitnle, ethylacetate and tetrahydrofuran A hemihydrate crystalline form (C) of the compound of this invention may be prepared from a 90% pure Form A (likely containing N-(5-cyclopropyl-1-quinolin-5- yl-1 H-pyrazole-4-carbonyl-guahdine, dimesylate salt as an impurity) by repulping in ethanol or isopropanol/water at a temperature of about 20°C to about 25°C, preferably ambient temperature for about 2 hours to about 24 hours, preferably with agitation. Typically the range is about 85% to about 95% ethanol and about 5% to about 15% water. Preferably the ratio is 91% to 9% ethanol/water. Alternative solvents include organic/water solvents such as acetonitrile, acetone, tetrahydrodrofuran, ethylacetate, with 5% to 10% water. The following Table 1 details important properties for four forms of: N-(5- cyclopropyl-1 -quinolin-5-yl-1 H-pyrazole-4-carbonyl)-guanidine, monomesylate salt: amorphous; the two anhydrous crystalline forms (A and B); and the hemihydrate crystalline form. TABLE 1 Those skilled in the art will recognize that the Formula I salt can exist in several tautomeric forms. All such tautomeric forms are considered as part of this invention. For example, all of the tautomeric forms of the carbonylguanidine moiety of the Formula I salt are included in this invention. Any aldose reductase inhibitor may be used as the second compound (active agent) of this invention for combination therapies. The term aldose reductase inhibitor refers to compounds which inhibit the bioconversion of glucose to sorbitol catalyzed by the enzyme aldose reductase. Such inhibition is readily determined by those skilled in the art according to standard assays (J. Malone, Diabetes. 29:861-864, 1980. "Red Cell Sorbitol, an Indicator of Diabetic Control"). A variety of aldose reductase inhibitors are known to those skilled in the art. An amount of the aldose reductase inhibitor that is effective for the activities of this invention may be used. Typically, an effective dosage for the aldose reductase inhibitors invention is in the range of about 0.1 mg/kg/day to 100 mg/kg/day in single or divided doses, preferably 0.1 mg/kg/day to 20 mg/kg/day in single or divided doses. Any glycogen phosphorylase inhibitor may be used as the second compound of this invention. The term glycogen phosphorylase inhibitor refers to any substance or agent or any combination of substances and/or agents which reduces, retards, or eliminates the enzymatic action of glycogen phosphorylase. The currently known enzymatic action of glycogen phosphorylase is the degradation of glycogen by catalysis of the reversible reaction of a glycogen macromolecule and inorganic phosphate to glucose-1-phosphate and a glycogen macromolecule which is one glucosyl residue shorter than the original glycogen macromolecule (forward direction of glycogenolysis). Such actions are readily determined by those skilled in the art according to standard assays. A variety of these compounds are included in the following published international patent applications: PCT application publication WO 96/39384 and W096/39385. However, other glycogen phosphorylase inhibitors will be known to those skilled in the art. In general an effective dosage of the glycogen phosphorylase inhibitor for the pharmacological combination compositions of this invention, for example the ischemic damage reducing activities of combinations containing the glycogen phosphorylase inhibitor compounds of this invention, is in the range of 0.005 to 50 mg/kg/day, preferably 0.01 to 25 mg/kg/day and most preferably 0.1 to 15 mg/kg/day. Those skilled in the art will recognize that other cardiovascular agents, for example, β-blockers (e.g., acebutolol, atenolol, bopindolol, labetolol, mepindolol, nadolol, oxprenol, pindolol, propranolol, sotalol), calcium channel blockers (e.g., amlodipine, nifedipine, nisoldipine, nitrendipine, verapamil), ACE inhibitors (e.g., captopril, enalapril), nitrates (e.g., isosorbide dinitrate, isosorbide 5-mononitrate, glyceryl trinitrate), diuretics (e.g., hydrochlorothiazide, indapamide, piretanide, xipamide), glycosides (e.g., digoxin, metildigoxin), thrombolytics (e.g. tPA), platelet inhibitors (e.g., reopro), aspirin, dipyridamol, potassium chloride, clonidme, prazosin, aldose reductase inhibitors (e g , zopolrestat) and adenosine A 3 receptor agonists may be used in conjunction with the crystals of this invention The crystals of the present invention inhibit the sodium/proton (Na+/H+) exchange transport system and hence are useful as a therapeutic or prophylactic agent for diseases caused by the acceleration of the sodium/proton (Na+/H+) exchange transport system, for example, cardiovascular diseases [e g , arteriosclerosis, hypertension, arrhythmia (e g , ischemic arrhythmia, arrhythmia due to myocardial infarction, arrhythmia after PTCA or after thrombolysis, etc ), angina pectons, cardiac hypertrophy, myocardial infarction, heart failure (e g , congestive heart failure, acute heart failure, cardiac hypertrophy, etc ), restenosis after PTCA, shock (e g hemorrhagic shock, endotoxin shock, etc )], renal diseases (e g , diabetes mellitus, diabetic nephropathy, ischemic acute renal failure, etc ) organ disorders associated with ischemia or ischemic reperfusion [(e g , heart muscle ischemic reperfusion associated disorders, acute renal failure, or disorders induced by surgical treatment such as coronary artery bypass grafting (CABG) surgeries, vascular surgeries, organ transplantation, non-cardiac surgeries or percutaneous transluminal coronary angioplasty (PTCA)], cerebrovascular diseases (e g , ischemic stroke, hemorrhagic stroke, etc ), cerebro ischemic disorders (e g , disorders associated with cerebral infarction, disorders caused after cerebral apoplexy as sequelae, or cerebral edema) The crystals of this invention can also be used as an agent for myocardial protection during coronary artery bypass grafting (CABG) surgeries, vascular surgeries, percutaneous transluminal coronary angioplasty (PTCA), organ transplantation, or non-cardiac surgeries Preferably, the crystals of this invention can be used as agents for myocardial protection before, during, or after coronary artery bypass grafting (CABG) surgeries, vascular surgeries percutaneous transluminal coronary angioplasty (PTCA), organ transplantation, or non-cardiac surgeries Preferably, the crystals of this invention can be used as agents for myocardial protection in patients presenting with ongoing cardiac (acute coronary syndromes, e g , myocardial infarction or unstable angina) or cerebral ischemic events (e g , stroke) Preferably, the crystals of this invention can be used as agents for chronic myocardial protection in patients with diagnosed coronary heart disease (e g , previous myocardial infarction or unstable angina) or patients who are at high risk for myocardial infarction (e g , age greater than 65 and two or more risk factors for coronary heart disease) In addition to this, the crystals of this invention are notable for their strong inhibitory effect on the proliferation of cells, for example the proliferation of fibroblast cells and the proliferation of the smooth muscle cells of the blood vessels For this reason, the crystals of this invention are valuable therapeutic agents for use in diseases in which cell proliferation represents a primary or secondary cause and may, therefore, be used as antiatherosclerotic agents, and as agents against diabetic late complications, cancerous diseases, fibrotic diseases such as pulmonary fibrosis, hepatic fibrosis or renal fibrosis, glomerular nephrosclerosis, organ hypertrophies or hyperplasias, in particular hyperplasia or hypertrophy of the prostate, pulmonary fibrosis, diabetic complications or recurrent stricture after PTCA, or diseases caused by endothehal cell injury The utility of the crystals of the present invention as medical agents in the treatment of diseases, such as are detailed herein in mammals (e g , humans) for example, myocardial protection during surgery or mycardial protection in patients presenting with ongoing cardiac or cerebral ischemic events or chronic cardioprotection in patients with diagnosed coronary heart disease, is demonstrated by the activity of the crystals of this invention in conventional preclinical cardioprotection assays [see the in vivo assay in Klein, H et al , Circulation 92 912-917 (1995), the isolated heart assay in Scholz, W et al , Cardiovascular Research 29 260-268 (1995), the antiarrhythmic assay in Yasutake M et al , Am J Physiol , 36 H2430-H2440 (1994), the NMR assay in Koike et al , J Thorac Cardiovasc Surg 112 765-775 (1996)] Such assays also provide a means whereby the activities of the crystals of this invention can be compared with the activities of other known crystals The results of these comparisons are useful for determining dosage levels in mammals including humans, for the treatment of such diseases Administration of the crystals of this invention can be via any method which delivers a crystal of this invention preferentially to the desired tissue (e g , liver and/or cardiac tissues) These methods include oral routes, parenteral, intraduodenal routes, etc Generally, the crystals of the present invention are admimstered in single (e g , once daily) or multiple doses or via constant infusion in, for example, an isotonic saline solution The crystals of this invention are useful, for example, in reducing or minimizing damage effected directly to any tissue that may be susceptible to ischemia/reperfusion injury (e g , heart, brain, lung, kidney, liver, gut, skeletal muscle, retina) as the result of an ischemic event (e g , myocardial infarction) The active compound is therefore usefully employed prophylactically to prevent, i e (prospectively or prophylactically) to blunt or stem, tissue damage (e g , myocardial tissue) in patients who are at risk for ischemia (e g , myocardial ischemia) Generally, the crystals of this invention are administered orally, or parenterally (e g , intravenous, intramuscular, subcutaneous or intramedullary) Topical administration may also be indicated, for example, where the patient is suffering from gastrointestinal disorders or whenever the medication is best applied to the surface of a tissue or organ as determined by the attending physician The amount and timing of crystals administered will, of course, be dependent on the subject being treated, on the severity of the affliction, on the manner of administration and on the judgement of the prescribing physician Thus, because of patient to patient variability, the dosages given below are a guideline and the physician may titrate doses of the drug to achieve the treatment that the physician considers appropriate for the patient In considering the degree of treatment desired, the physician must balance a variety of factors such as age of the patient, presence of preexisting disease, as well as presence of other diseases (e g , cardiovascular disease) Thus, for example, in one mode of administration the crystals of this invention may be administered just prior to cardiac surgery (e g , within twenty-four hours before surgery), during or subsequent to cardiac surgery (e g , within twenty- four hours after surgery) where there is risk of myocardial ischemia in an especially preferred mode an infusion is administered with a loading dose of about 1 mg to about 300 mg for about one minute to about one hour prior to surgery followed by a constant infusion of about 1 mg/kg/day to about 100 mg/kg/day for the remaining presurgery, surgery and post surgery periods, including for example about 2 to about 7 days post surgical treatment The compounds of this invention may also be administered in a chronic daily mode An amount of the crystals of this invention is used that is effective for ischemic protection A preferred dosage is about 0 001 to 100 mg/kg/day of the crystal of this invention An especially preferred dosage is about 0 01 to 50 mg/kg/day of the crystal of this invention The crystals of the present invention are generally administered in the form of a pharmaceutical composition comprising at least one of the crystals of this invention together with a pharmaceutically acceptable vehicle or diluent Thus, the crystals of this invention can be administered individually or together in any conventional oral, parenteral, rectal or transdermal dosage form For oral administration a pharmaceutical composition can take the form of solutions, suspensions, tablets, pills, capsules, powders, and the like Tablets containing various excipients such as sodium citrate, calcium carbonate and calcium phosphate are employed along with various disintegrants such as starch and preferably potato or tapioca starch and certain complex silicates, together with binding agents such as polyvinylpyrrolidone, sucrose, gelatin and acacia Additionally, lubricating agents such as magnesium stearate, sodium lauryl sulfate and talc are often very useful for tabletting purposes Solid compositions of a similar type are also employed as fillers in soft and hard-filled gelatin capsules, preferred materials in this connection also include lactose or milk sugar as well as high molecular weight polyethylene glycols When aqueous suspensions and/or elixirs are desired for oral administration, the compounds of this invention can be combined with various sweetening agents, flavoring agents, coloring agents, emulsifying agents and/or suspending agents, as well as such diluents as water, ethanol, propylene glycol, glycerin and various like combinations thereof For purposes of parenteral administration, solutions, for example, in sesame or peanut oil or in aqueous propylene glycol can be employed, as well as sterile aqueous solutions of the corresponding water-soluble salts Such aqueous solutions may be suitably buffered, if necessary, and the liquid diluent first rendered isotonic with sufficient saline or glucose These aqueous solutions are especially suitable for intravenous, intramuscular, subcutaneous and intrapentoneal injection purposes In this connection, the sterile aqueous media employed are all readily obtainable by standard techniques well-known to those skilled in the art For purposes of transdermal (e g .topical) administration, dilute sterile, aqueous or partially aqueous solutions (usually in about 0 1 % to 5% concentration), otherwise similar to the above parenteral solutions, are prepared Methods of preparing various pharmaceutical compositions with a certain amount of active ingredient are known, or will be apparent in light of this disclosure, to those skilled in this art For examples of methods of preparing pharmaceutical compositions, see Remington's Pharmaceutical Sciences, Mack Publishing Company, Easter, Pa , 15th Edition (1975) Pharmaceutical compositions according to the invention may contain for example 0 0001 %-95% of the compound(s) of this invention In any event, the composition or formulation to be administered will contain a quantity of a crystals(s) according to the invention in an amount effective to treat the disease/condition of the subject being treated The crystals of this invention generally will be administered in a convenient formulation The following formulation examples are illustrative only and are not intended to limit the scope of the present invention In the formulations which follow, "active ingredient" means a compound(s) (crystals(s)) of this invention Formulation 1 Gelatin Capsules Hard gelatin capsules are prepared using the following Ingredient Quantity (mg/capsule) Active ingredient 0 25-100 Starch, NF 0-650 Starch flowable powder 0-50 Sihcone fluid 350 centistokes 0-15 A tablet formulation is prepared using the ingredients below Formulation 2 Tablets Ingredient Quantity (mg/tablet) Active ingredient 0 25-100 Cellulose, microcrystalline 200-650 Silicon dioxide, fumed 10-650 Stearate acid 5-15 The components are blended and compressed to form tablets Alternatively, tablets each containing 0 25-100 mg of active ingredients are made up as follows Formulation 3 Tablets Ingredient Quantity (mg/tablet) Active ingredient 0 25-100 Starch 45 Cellulose, microcrystalline 35 Polyvinylpyrrohdone (as 10% solution in water) 4 Sodium carboxymethyl cellulose 4 5 Magnesium stearate 0 5 Talc 1 The active ingredient, starch, and cellulose are passed through a No 45 mesh U S sieve and mixed thoroughly The solution of polyvinylpyrrohdone is mixed with the resultant powders which are then passed through a No 14 mesh U S sieve The granules so produced are dried at 50° - 60°C and passed through a No 18 mesh U S sieve The sodium carboxymethyl starch, magnesium stearate, and talc, previously passed through a No 60 U S sieve, are then added to the granules which, after mixing, are compressed on a tablet machine to yield tablets Suspensions each containing 0 25-100 mg of active ingredient per 5 ml dose are made as follows Formulation 4 Suspensions Ingredient Quantity (mg/5 ml) Active ingredient 0 25-100 mg Sodium carboxymethyl cellulose 50 mg Syrup 1 25 mg Benzoic acid solution 0 10 mL Flavor q v Color q v Purified Water to 5 mL The active ingredient is passed through a No 45 mesh U S sieve and mixed with the sodium carboxymethyl cellulose and syrup to form smooth paste The benzoic acid solution, flavor, and color are diluted with some of the water and added, with stirring. Sufficient water is then added to produce the required volume. An aerosol solution is prepared containing the following ingredients: Formulation 5: Aerosol Ingredient Quantity (% by weight) Active ingredient 0.25 Ethanol 25.75 Propellant 22 (Chlorodifluoromethane) 74.00 The active ingredient is mixed with ethanol and the mixture added to a portion of the propellant 22, cooled to 30°C, and transferred to a filling device. The required amount is then fed to a stainless steel container and diluted with the remaining propellant. The valve units are then fitted to the container. Suppositories are prepared as follows: Formulation 6: Suppositories Ingredient Quantity (mg/suppository) Active ingredient 250 Saturated fatty acid glycerides 2,000 The active ingredient is passed through a No. 60 mesh U.S. sieve and suspended in the saturated fatty acid glycerides previously melted using the minimal necessary heat. The mixture is then poured into a suppository mold of nominal 2 g capacity and allowed to cool. An intravenous formulation is prepared as follows: Formulation 7: Intravenous Solution Ingredient Quantity Active ingredient 25 mg Isotonic saline 1 ,000 mL The solution of the above ingredients is intravenously administered to a patient. The active ingredient above may also be a combination of agents. EXAMPLE 1 Methyl-3-cyclopropyl-3-oxopropanoate (15 g, 106 mmol, 1 equiv) and N,N- dimethylformamide dimethylacetal (14.7 mL, 1 11 mmol, 1.05 equiv) were heated at 75 °C for 1 5 h under N 2 The orange oil was then cooled to room temperature TLC analysis (1 1 EtOAc/hexanes) indicated disappearance of starting material and appearance of a minor less polar spot and a major more polar spot (methyl-3- cyclopropyl-2-dιmethylenamιno-3-oxopropanoate) The crude mixture was used as EXAMPLE 2 Crude methyl-3-cyclopropyl-2-dιmethylenamιno-3-oxopropanoate (20 9 g, 106 mmol, 1 07 equiv) was diluted with ethanol (250 mL) Tπethylamine (34 4 mL, 247 mmol, 2 5 equiv) followed by quιnolιn-5-yl-hydrazιne (22 9 g, 98 6 mmol, 1 equiv) was added sequentially Slight gas evolution upon addition of quιnolιn-5-yl-hydrazιne was observed The resulting heterogeneous mixture was heated at reflux (78 °C) under N 2 for 2 h The mixture became homogeneous and very dark after about 3 mm of heating The mixture was then cooled to room temperature TLC analysis (1 1 EtOAc/hexanes) indicated a slightly less polar spot (5-cyclopropyl-1-quιnohn-5-yl-1 H-pyrazole-4- carboxylic acid methyl ester) APCI mass spec indicated desired product as well The reaction mixture was then concentrated To the residue was added EtOAc (300 mL) and 0 1 N HCI (400 mL) This emulsion was stirred for 10 mm at room temperature and then filtered through a pad of Cehte® to remove solids The resulting biphasic mixture was separated The aqueous layer was extracted with EtOAc (2 X 300 mL) The combined organic layers were washed with 0 1 N HCI (2 X 300 mL), then dried over sodium sulfate, and concentrated To the residue was added hot isopropyl ether (80 mL) The cloudy solution was stirred for 2 mm Then hexanes (125 mL) were added The solids were allowed to granulate overnight Solids were collected by filtration to provide the product, 5-cyclopropyl-1-quιnohn-5-yl-1 H-pyrazole-4-carboxyhc acid methyl ester, as a yellow orange powder (20 8 g, 72% over 2 steps) EXAMPLE 3 To a solution of 5-cyclopropyl-1-quιnolιn-5-yl-1 H-pyrazole-4-carboxyhc acid methyl ester (20 g, 68 2 mmol, 1 equiv) in MeOH (120 mL) was added 2N NaOH (54 5 mL, 109 mmol, 1 6 equiv) The resulting solution was heated at reflux (65 °C) for 1 5 h under N 2 , and then allowed to cool to room temperature TLC analysis (1 1 EtOAc/hexanes) indicated disappearance of starting material The methanol was removed under vacumn with gentle heating (35 °C) on a rotovap The basic aqueous layer was then washed with EtOAc (2 X 100 mL) The resulting basic aqueous layer was acidified slowly to pH 1-2 with concentrated HCI The product precipitated out during acidification The slurry was stirred at room temperature for 0 5 h, then the solids were collected by filtration The solids were washed with 1 N HCI (2 X 25 mL) and dried to afford the acid as a pale brown solid (18 8 g, 99%) EXAMPLE 4 To a stirred suspension of 5-cyclopropyl-1-quιnolιn-5-yl-1 H-pyrazole-4- carboxyhc acid (25 g, 89 5 mmol, 1 equiv) in toluene (250 mL) was added thionyl chloride (32 6 mL, 448 mmol, 5 equiv) The resulting suspension was heated at 75 °C for 1 5 h under N 2 The reaction mixture stayed heterogeneous throughout The solid acid chloride was collected by filtration The tan solid was washed with toluene (3 X 50 mL) and dried under vacumn A suspension of the acid chloride in THF (250 mL) was cooled to 0 °C A solution of guanidine hydrochlonde (17 1 g, 179 mmol, 2 equiv) and 2N NaOH (224 mL, 448 mmol, 5 equiv) was added via a dropping addition funnel over 5-10 mm under N 2 The reaction became homogenous and biphasic upon addition of the basic aqueous solution of guanidine The mixture was stirred at 0 °C with slow warming over 1 h to room temperature and then for an additional 1 h at room temperature TLC analysis (4 1 dichloromethane/methanol) indicated appearance of a more polar spot (N-(5-cyclopropyl-1-quιnolιn-5-yl-1 H-pyrazole-4-carbonyl)- guanidine) and trace starting material acid THF was removed under vacumn with gentle heating (35 °C) which resulted in precipitation of the product The aqueous layer was stirred at room temperature for 1 h to allow the product to granulate The solid was collected by filtration, washed with water (2 X 50 mL), and dried The color of the product has ranges from off-white to medium brown This batch was medium brown Reslurry in MeOH (125 mL) for 30 mm provided the desired product N-(5-cyclopropyl-1-quιnolιn-5-yl-1H-pyrazole-4-carbonyl)-guanιdιne (22 6 g, 79% yield) as a pale tan solid EXAMPLE 5 N-(5-Cyclopropyl-1-qumohn-5-yl-1 H-pyrazole-4-carbonyl)-guanιdιne (3 08 kg, 70 5 9 61 mmol 1 equiv) was suspended in acetone (30 8 kg) 1-Methyl-2-pyrrohdιnone (12 3 kg) was added to obtain a homogenenous solution An additional 4 8 kg of acetone was used to rinse forward (spec free filtration) The reaction solution was warmed to 50°C A solution of methanesulfonic acid (0 83 kg, 8 65 mol, 0 9 equiv) in acetone (8 3 kg) was added while keeping the temperature below 55°C The slurry that was obtained was agitated at 50°C for 1-2 hours, then cooled, and filtered The filter cake was rinsed with acetone and then dried to afford N-(5- cyclopropyl-1 -quιnohn-5-yl-1 H-pyrazole-4-carbonyl)-guanιdιne, monomesylate salt (3 24 kg, 81 %) as an off-white solid The product was then dried under vacuum affording an anhydrous crystal having the following properties (Form D) Microscopy birefnngent plate/equant Degree of Crystallmity fully crystalline Hygroscopicity non-hygroscopic Appearance off white crystalline solid Melting point 215°C (onset temperature at 5°C/mιn) The X-Ray diffraction d-spacing is provided in the following Table II TABLE II Form D Anode CU - Wavelength 1 1 54056 Wavelength 2 1 54439 (Rel Intensity 0 500) EXAMPLE 6 To 3.165 kgs of the product of Example 5 was added 123 Liters (3.8 volumes) of acetone. The slurry was agitated for 20 hours at room temperature. The slurry was filtered, and solids were dried at 50°C. The product was an anhydrous crystal (3.145 kg, 99%) (Form A) having the following properties. Microscopy: birefringent equant Degree of Crystallinity: fully crystalline Hygroscopicity: non-hygroscopic Appearance: white crystalline solid Melting point: 228°C (onset temperature at 5°C/min) The X-Ray diffraction d-spacing is provided in the following Table III TABLE III FORM A EXAMPLE 7 To 0.25 gram of 1 ml of an approx. 90% pure sample of Form A (likely containing N-(5-cyclopropyl-1 -quinolin-5-yl-1 H-pyrazole-4-carbonyl)-guanidine, dimesylate as an impurity) ethanol was added (EtOH contained -9% water). The slurry was agitated for about 21 hours at room temperature. The slurry was then filtered, and the solid was dried at 50°C under vacuum for 90 minutes affording a hemihydrate crystal (Form C) having the following properties. Microscopy: biref ngent equant Degree of Crystallinity: fully crystalline Appearance: cream-colored crystalline solid Melting point: 140°C - 170°C desolvation, 209°C (onset temperature at 5°C/min) The X-Ray diffraction d-spacing is provided in the following Table IV TABLE IV Form C Anode: CU - Wavelength 1 1.54056 Wavelength 2: 1.54439 (Rel lntensity:0.500) Range # 1 - Coupled: 3.000 to 40.000 StepSize: 0.040 StepTime: 1.00

Claims: CLAIMS 1. A crystalline form of a salt having the Formula 2. A crystal which is N-(5-cyclopropyl-1-quinolin-5-yl-1 H-pyrazole-4-carbonyl)- guanidine, monomesylate salt. 3. A crystal of claim 1 which is anhydrous N-(5-cyclopropyl-1-quinolin-5-yl-1 H- pyrazole-4-carbonyl)-guanidine, monomesylate salt. 4. A crystal of claim 1 which is anhydrous N-(5-cyclopropyl-1-quinolin-5-yl-1 H- pyrazole-4-carbonyl)-guanidine, monomesylate salt (D) having the following X-ray diffraction pattern Form D Anode: CU - Wavelength 1 : 1.54056 Wavelength 2: 1.54439 (Rel lntensity:0.500) Range # 1 - Coupled: 3.000 to 40.000 StepSize: 0.040 StepTime: 1.00 5. A crystal of claim 1 which is the anhydrous N-(5-cyclopropyl-1-quinolin-5-yl- 1 H-pyrazole-4-carbonyl)-guanidine, monomesylate salt (A) having the following X- ray diffraction pattern FORM A 6 A crystal of claim 1 which is N-(5-cyclopropyl-1-quιnolιn-5-yl-1 H-pyrazole-4- carbonyl)-guanιdιne, monomesylate hemihydrate 7 A method for making the crystal of claim 4 comprising combining an acetone solution of N-(5-cyclopropyl-1-qumolιn-5-yl-1H-pyrazole-4-carbonyl)- guanidme and N-methyl pyrrolidinone with methanesulfonic acid at a temperature of about 30°C to about 60°C, followed by cooling to a temperature of about ambient to about 35°C 8 A method for making the crystal of claim 5 comprising repulpmg the Form (D) anhydrous crystal in acetone at a temperature of about 20°C to about 25°C wherein said anhydrous Form D crystal has the following X-ray diffraction pattern Form D Anode CU - Wavelength 1 1 54056 Wavelength 2 1 54439 (Rel Intensity 0 500) 9 A method for making the crystal of claim 6 comprising repulpmg the anhydrous Form A crystal in ethanol or iso-propanol/water at a temperature of about 20°C to about 25°C, wherein said anhydrous Form A crystal has the following X-ray diffraction pattern FORM A Anode CU - Wavelength 1 1 54056 Wavelength 2 1 5443 (Rel Intensity 0 500) Range # 1 - Coupled 3 000 to 40 000 StepSize 0 040 StepTime 1 00 10 The method as recited in claim 9 wherein the range is about 85% to about 95% ethanol and about 5% to about 15% water 11 [5-Cyclopropyl-1 -(quιnolιn-5-yl)-1 H-pyrazole-4-carbonyl]guanιdιne, monomesylate hemihydrate 12 A method of reducing tissue damage resulting from ischemia or hypoxia comprising administering to a mammal in need of such treatment a therapeutically effective amount of a pharmaceutical composition wherein said composition comprises a crystal of claim 1 and a pharmaceutically acceptable carrier, vehicle or diluent 13 A method as recited in claim 12 wherein the tissue is cardiac, brain, liver kidney, lung, gut, skeletal muscle, spleen pancreas nerve, spinal cord, retina tissue, the vasculature, or intestinal tissue 14 A method as recited in claim 13 wherein the amount of the Formula I crystal is about 0 01 mg/kg/day to about 10 mg/kg/day 15 A method as recited in claim 14 wherein the mammal is a female or male human 16 A method as recited in claim 15 wherein said tissue is heart tissue 17 A method as recited in claim 15 wherein said tissue is brain tissue 18 A method as recited in claim 15 wherein said tissue is liver tissue 19 A method as recited in claim 15 wherein said tissue is kidney tissue 20 A method as recited in claim 15 wherein said tissue is lung tissue 21 A method as recited in claim 15 wherein said tissue is gut tissue 22 A method as recited in claim 15 wherein said tissue is skeletal muscle tissue 23 A method as recited in claim 15 wherein said tissue is spleen tissue 24 A method as recited in claim 15 wherein said tissue is pancreas tissue 25 A method as recited in claim 15 wherein said tissue is retina tissue 26 A method as recited in claim 15 wherein the compound is administered prophylactically 27 A method as recited in claim 15 wherein the pharmaceutical composition is administered prior to surgery 28 A method as recited in claim 15 wherein the pharmaceutical composition is administered prior to cardiac surgery 29 A method as recited in claim 15 wherein the pharmaceutical composition is administered during surgery 30 A method as recited in claim 15 wherein the pharmaceutical composition is administered during cardiac surgery 31 A method as recited in claim 15 wherein the pharmaceutical composition is administered within twenty-four hours after surgery 32 A method as recited in claim 15 wherein the pharmaceutical composition is administered within twenty four hours after cardiac surgery 33 A method as recited in claim 15 wherein the tissue damage resulting from ischemia is ischemic damage and is incurred during organ transplantation 34 A method as recited in claim 15 wherein the pharmaceutical composition is administered to prevent penoperative myocardial ischemic injury 35 A pharmaceutical composition which comprises a therapeutically effective amount of a crystal of claim 1 and a pharmaceutically acceptable carrier, vehicle or diluent 36 A pharmaceutical composition for the reduction of tissue damage resulting from ischemia or hypoxia which comprises a therapeutically effective amount of a crystal of claim 1 and a pharmaceutically acceptable carrier, vehicle or diluent 37 A method as recited in claim 15 wherein the pharmaceutical composition is administered prior to, during and after surgery 38 A method as recited in claim 15 wherein the pharmaceutical composition is administered prior to, during and after cardiac surgery

Inventors: BROSTROM LYLE ROBINSON, CONNOLLY TERRENCE JOSEPH, LI ZHENG JANE, ORRILL SUSAN LEE, SHAH BHARAT KIRITKUMAR, BROSTROM, LYLE, ROBINSON, CONNOLLY, TERRENCE, JOSEPH, LI, ZHENG, JANE, ORRILL, SUSAN, LEE, SHAH, BHARAT, KIRITKUMAR
Applicants: BROSTROM LYLE ROBINSON, CONNOLLY TERRENCE JOSEPH, LI ZHENG JANE, ORRILL SUSAN LEE, PFIZER PROD INC, SHAH BHARAT KIRITKUMAR, BROSTROM, LYLE, ROBINSON, CONNOLLY, TERRENCE, JOSEPH, LI, ZHENG, JANE, ORRILL, SUSAN, LEE, PFIZER PRODUCTS INC., SHAH, BHARAT, KIRITKUMAR
Application year: 2000
Main classification: C07D231
IPC codes: A61K31, A61P3, A61P9, A61P11, A61P13, A61P21, A61P35, A61P39, A61P41, A61P43, B01D9, C07D231, C07D401