ANTIMICROBIAL WIPES

Abstract: An antimicrobial wipe comprises a porous or absorbent sheet impregnated with an antimicrobial cleansing composition, wherein the antimicrobial cleansing composition comprises from about 0.001 % to about 5.0 %, by weight of the antimicrobial cleansing composition, of an antimicrobial active; from about 0.05 % to about 10 %, by weight of the antimicrobial cleansing composition, of an anionic surfactant; from about 0.1 % to about 10 %, by weight of the antimicrobial cleansing composition, of a proton donating agent; and from about 3 % to about 99.85 %, by weight of the antimicrobial cleansing composition, water; wherein the composition is adjusted to a pH of from about 3.0 to about 6.0; wherein the antimicrobial cleansing composition has a Gram Negative Residual Effectiveness Index of greater than about 0.3. Also disclosed are methods for cleansing skin and providing residual effectiveness versus Gram negative bacteria using these products.

Description: ANTIMICROBIAL WIPES TECHNICAL FIELD The present invention relates to cleansing wipes compπsing absorbent sheets impregnated with antimicrobial cleansing compositions These cleansing wipes provide enhanced antimicrobial effectiveness compared to prior art compositions. Specifically, the cleansing wipe personal cleansing compositions of the invention provide previously unseen residual effectiveness against transient Gram negative bactena. BACKGROUND OF THE INVENTION Human health is impacted by many microbial entities. Inoculation by viruses and bacteria cause a wide vanety of sicknesses and ailments Media attention to cases of food poisoning, strep infections, and the like is increasing public awareness of microbial issues. It is well known that the washing of hard surfaces, food (e.g. fruit or vegetables) and skin, especially the hands, with antimicrobial or non-medicated soap, can remove many viruses and bactena from the washed surfaces. Removal of the viruses and bactena is due to the surfactancy of the soap and the mechanical action of the wash procedure. Therefore, it is known and recommended that the people wash frequently to reduce the spread of viruses and bacteria. Bacteria found on the skm can be divided into two groups: resident and transient bacteria Resident bactena are Gram positive bacteria which are established as permanent microcolomes on the surface and outermost layers of the sk and play an important, helpful role in preventing the colonization of other, more harmful bactena and fungi. Transient bactena are bacteria which are not part of the normal resident flora of the skm, but can be deposited when airborne contaminated material lands on the skm or when contaminated material is brought into physical contact with it Transient bacteria are typically divided into two subclasses Gram positive and Gram negative Gram positive bacteria include pathogens such as Staphylococcus aureus. Streptococcus pyogenes and Clostridium botuhnum Gram negative bacteria include pathogens such as Salmonella, Escherichia coli, Klebsiella, Haemophilus, Pseudomonas aeruginosa, Proteus and Shigella dysenteriae Gram negative bacteria are generally distinguished from Gram positive by an additional protective cell membrane which generally results in the Gram negative bactenabemg less susceptible to topical antibactenal actives. Antimicrobial cleansing products have been marketed in a vanety of forms for some time. Forms include deodorant soaps, hard surface cleaners, and surgical disinfectants. These traditional nnse-off antimicrobial products have been formulated to provide bactena removal dunng washing. The antimicrobial soaps have also been shown to provide a residual effectiveness against Gram positive bacteria, but limited residual effectiveness versus Gram negative bacteria. By residual effectiveness it is meant that bactena growth on a surface is controlled for some period of time following the washmg/ nsing process. Antimicrobial liquid cleansers are disclosed in U.S. Patent Numbers: 4,847,072, Bissett et al., issued July 11, 1989, 4,939,284, Degenhardt, issued July 3, 1990 and 4,820,698, Degenhardt, issued Apnl 11, 1989, all patents being incorporate herein by reference. Some of these traditional products, especially the hard surface cleaners and surgical disinfectants, utilize high levels of alcohol and/or harsh surfactants which have been shown to dry out and irritate skm tissues. Ideal personal cleansers should gently cleanse the skm, cause little or no lrntation, and not leave the skin overly dry after frequent use and preferably should provide a moistunzmg benefit to the skm. Finally, these traditional antimicrobial compositions have been developed for use in a washing process with water. This limits their use to locations with available water. Cleansing wipes have been used, m the past, to wash hands and face while traveling or in public or anytime water is not available. In fact, consumers have used absorbent sheets impregnated with topical compositions for a vanety of purposes. U.S. Patent Number 4,045,364, Richter, et al., issued August 30, 1977 teaches a dry disposable paper impregnated with a germicidal composition compnsmg an amonic surfactant, an elemental iodine or lodophor active ingredient and a weak acid for pH adjustment. The compositions utilize iodine actives which are not stable in the presence of substantial amounts of water and insufficient acid levels to provide the residual effectiveness versus Gram negative bacteria. European Patent Application, EP 0 619 074, Touchet et al., published October 12, 1994, teaches the use of sorbic or benzoic acids as antimicrobial agents m a wipe, however does not teach the amonic surfactant and separate antimicrobial active necessary to achieve the residual effectiveness of the present invention. U.S Patent Number 4,975,217, Brown-Skrobot et al., issued December 4, 1990 teaches the use of amonic surfactants and organic acids on a wipe, however does not teach the use of the active required to provide the residual effectiveness versus Gram negative bacteria. Currently marketed Nice'n Clean®, ash'n Dry® and No More Germies® are all antibacterial wipes which utilize harsh catiomc surfactants with no additional antibacterial active These products do not provide the residual effectieness versus Gram negative bacteria PCT application WO 92/18100, Keegan et al., published October 29, 1992 and PCT application WO 95/32705, Fujiwara et al., published December 7, 1995 teach non-wipe liquid skm cleansers compnsmg mild surfactants, antibacterial agents and acidic compounds to buffer the pH, which provide improved germ hostility However, the use of the acid compounds for only pH adjustment therein, results in compositions which do not deliver the undissociated acid required to provide residual effectiveness versus Gram negative bactena. This situation is compounded m Keegan and Fujiwara by the preference of mild surfactants, including noniomc surfactants. Neither Keegan nor Fujiwara teach the use of their compositions in a form which can be used without available water, e.g., a wipe. U.S. Patent Number 3,141,821, issued to Compeau, July 21, 1964 and Irgasan DP 300 (Tnclosan®) technical literature from Ciba-Giegy, Inc., "Basic Formulation for Hand Disinfection 89/42/01" set forth antibacterial skm cleanser compositions which could provide improved residual effectiveness versus Gram negative bacteria using certain amonic surfactants, anti-microbial actives and acids. However, the selection of highly active surfactants results in personal cleansing compositions which are drying and harsh to the skm. Again, neither reference teaches the use of antimicrobial compositions in a form which can be used without available water, e.g. a wipe. Given the severe health impacts of Gram negative bacteria like Salmonella, Escherichia coli and Shigella, it would be highly desirable to formulate antimicrobial cleansing products which provides residual effectiveness versus these transient Gram negative bacteria, which are mild to the skm and which can be used without water. Existing products have been unable to deliver all of these benefits Applicants have found that antimicrobial wipes which provide such mildness and residual effectiveness versus Gram negative bacteria can be formulated by using known porous or absorbent sheets which are impregnated with improved antimicrobial cleansing compositions These improved antimicrobial cleansing compositions contain antibacterial actives in combination with specific organic and/or inorganic acids as proton donating agents, and specific amonic surfactants, all of which are deposited on the skin. The deposited proton donating agent and amonic surfactant enhance the the selected active, to provide a new level of hostility to bacteria contacting the skm. SUMMARY OF THE INVENTION The present invention relates to an antimicrobial wipe comprising a porous or absorbent sheet impregnated with an antimicrobial cleansing composition, wherein the antimicrobial cleansing composition compnses from about 0.001% to about 5.0%, by weight of the antimicrobial cleansing composition, of an antimicrobial active; from about 0.05% to about 10%, by weight of the antimicrobial cleansing composition, of an amonic surfactant; from about 0.1% to about 10%, by weight of the antimicrobial cleansing composition, of a proton donating agent; and from about 3% to about 99.85%, by weight of the antimicrobial cleansing composition, water; wherein the composition is adjusted to a pH of from about 3.0 to about 6.0; wherein the antimicrobial cleansing composition has a Gram Negative Residual Effectiveness Index of greater than about 0.3. The present invention also relates to an improved antimicrobial cleansing composition which also has a Mildness Index of greater than about 0.3. The present invention also relates to methods for cleansing and decreasing the spread of transient Gram negative bacteria using the antimicrobial wipes described herein. DETAILED DESCRIPTION OF THE INVENTION The antimicrobial wipes of the present invention are highly efficacious for providing a residual antimicrobial effectiveness versus transient Gram negative bacteria, are mild to the skin and can be used without additional available water. The term "antimicrobial wipe" is used herein to mean products in which a sheet of porous or absorbent material have been impregnated with an antimicrobial cleansing composition for the purpose of rubbing the wipe product over a surface to clean the surface and control the growth and viability of transient bacteria. The term "antimicrobial cleansing composition" as used herein means a composition suitable for application to the human skin for the purpose of removing dirt, oil and the like, which additionally controls the growth and viability of transient bacteria on the skin. The composiitons of the present invention can also be useful for treatment of acne. As used herein "treating acne" means preventing, retarding and/or arresting the process of acne formation in mammalian skin. The compositions of the invention can also potentially be useful for providing an essentially immediate (i.e., acute) visual improvement in skin appearance following application of the composition to the skin. More particularly, the compositions of the present invention are useful for regulating skin condition, including regulating visible and/or tactile discontinuities in skin, including but not limited to visible and or tactile discontinuities in skin texture and/or color, more especially discontinuities associated with skin aging. Such discontinuities may be induced or caused by internal and/or external factors. Extrinsic factors include ultraviolet radiation (e.g., from sun exposure), environmental pollution, wind, heat, low humidity, harsh surfactants, abrasives, and the like. Intrinsic factors include chronological aging and other biochemical changes from within the skin. Regulating skin condition includes prophylactically and/or therapeutically regulating skin condition. As used herein, prophylactically regulating skin condition includes delaying, minimizing and/or preventing visible and/or tactile discontinuities in skin. As used herein, therapeutically regulating skin condition includes ameliorating, e.g., diminishing, minimizing and/or effacing, such discontinuities Regulating skm condition involves improving skm appearance and/or feel, e.g., providing a smoother, more even appearance and/or feel. As used herein, regulating skm condition includes regulating signs of agmg "Regulating signs of skm agmg" includes prophylactically regulating and/or therapeutically regulating one or more of such signs (similarly, regulating a given sign of skin agmg, e g., lines, wnnkles or pores, includes prophylactically regulating and/or therapeutically regulating that sign). "Signs of skm agmg" include, but are not limited to, all outward visibly and tactilely perceptible manifestations as well as any other macro or micro effects due to skin agmg Such signs may be induced or caused by intrinsic factors or extrinsic factors, e g., chronological agmg and/or environmental damage. These signs may result from processes which include, but are not limited to, the development of textural discontinuities such as wrinkles, including both fine superficial wnnkles and coarse deep wnnkles, skm lines, crevices, bumps, large pores (e.g , associated with adnexal structures such as sweat gland ducts, sebaceous glands, or hair follicles), scahness, flakiness and/or other forms of skm unevenness or roughness, loss of skm elasticity (loss and/or mactivation of functional skm elastm), sagging (including puffiness m the eye area and jowls), loss of skm firmness, loss of skm tightness, loss of skm recoil from deformation, discoloration (including undereye circles), blotching, sallowness, hyperpigmented skm regions such as age spots and freckles, keratoses, abnormal differentiation, hyperkeratmization, elastosis, collagen breakdown, and other histological changes in the stratum corneum, dermis, epidermis, the skm vascular system (e g., telangiectasia or spider vessels), and underlying tissues, especially those proximate to the skm. All percentages and ratios used herein, unless otherwise indicated, are by weight and all measurements made are at 25°C, unless otherwise designated The invention hereof can comprise, consist of, or consist essentially of, the essential as well as optional ingredients and components descnbed therein The antimicrobial wipes of the present invention comprise the following essential components. A THE POROUS OR ABSORBENT SHEET The antimicrobial cleansing composition is impregnated at the desired weight onto one or both sides of an absorbent sheet (hereinafter sometimes referred to as "substrate") which may be formed from any woven or nonwoven fiber, fiber mixture or foam of sufficient wet strength and absorbency to hold an effective amount of the antimicrobial cleansing composition. It is preferred from the standpoint of antimicrobial effectiveness and mildness to employ substrates with a high absorbent capacity (e g , from about 5 to about 20 grams/gram, preferably from about 9 to about 20 grams/gram) The absorbent capacity of a substrate is the ability of the substrate, while supported honzontally, to hold liquid The absorbent capacity of a substrate is measured according to the Absorbent Capacity Method set forth hereinafter in the Analytical Methods section. In particular, woven or nonwoven fabrics derived from "oriented" or carded fibrous webs composed of textile-length fibers, the major proportion of which are oriented predominantly in one direction are suitable for use herein. These fabrics can be in the form of, for example, wipes or towelettes, including baby wipes and the like. Methods of making woven and nonwoven cloths are not a part of this invention and, being well known in the art, are not described in detail herein. Generally, however, such cloths are made by air- or water-laying processes in which the fibers or filaments are first cut to desired lengths from long strands, passed into a water or air stream, and then deposited onto a screen through which the fiber-laden air or water is passed. The deposited fibers or filaments are then adhesively bonded together, and otherwise treated as desired to form the woven, nonwoven, or cellulose cloth. Thermocarded nonwoven cloths (whether or not resin-containing) are made of polyesters, polyamides, or other thermoplastic fibers which can be spand bonded, i.e., the fibers are spun out onto a flat surface and bonded (melted) together by heat or chemical reactions. The nonwoven cloth substrates used in the invention herein are generally adhesively bonded fibers or filamentous products having a web or carded fiber structure (when the fiber strength is suitable to allow carding) or comprising fibrous mats in which the fibers or filaments are distributed haphazardly or in random array (i.e., an array of fibers in a carded web where partial orientation of the fibers is frequently present, as well as a completely haphazard distributional orientation), or substantially aligned. The fibers or filaments can be natural (e.g., wool, silk, jute, hemp, cotton, linen, sisal, or ramie) or synthetic (e.g., rayon, cellulose ester, polyvinyl derivatives, polyolethins, polyamides, or polyesters) as have been described hereinabove. These nonwoven materials are generally described in Riedel "Nonwoven Bonding Methods and Materials", Nonwoven World. (1987). The absorbent properties preferred herein are particularly easy to obtain with nonwoven cloths and are provided merely by building up the thickness of the cloth, i.e., by superimposing a plurality of carded webs or mats to a thickness adequate to obtain the necessary absorbent properties, or by allowing a sufficient thickness of the fibers to deposit on the screen. Any denier of the fiber (generally up to about 15 denier) can be used, inasmuch as it is the free space between each fiber that makes the thickness of the cloth directly related to the absorbent capacity of the cloth. Thus, any thickness necessary to obtain the required absorbent capacity can be used. B. THE ANTIMICROBIAL CLEANSING COMPOSITION The absorbent sheets used in the present invention are impregnated with an antimicrobial cleansing composition. The term "antimicrobial cleansing composition" as used herein means a composition suitable for application to a surface for the purpose of removing dirt, oil and the like which additionally controls the growth and viability of transient Gram negative bacteria. Preferred embodiments of the present invention are cleansing compositions suitable for use on the human skin. I. INGREDIENTS The antimicrobial cleansing compositions of the wipes of the present invention comprise an antimicrobial active, an anionic surfactant, and a proton donating agent. These components are selected so that the efficacy and optional mildness requirements hereinafter defined for the compositions herein are met. The selection of each component is necessarily dependent on the selection of each of the other components. For example, if a weak acid is selected as the proton donating agent, then in order to realize an efficacious composition, either a more biologically active (but possibly less mild) surfactant must be employed, and/or a high level of acid within the prescribed range must be used and/or a particularly efficacious active must be employed. Similarly, if a mild, but nonefficacious surfactant is employed, then a stronger acid and/or a high level of acid may be necessary to realize an efficacious composition. If a harsh surfactant is utilized, then a mildness agent may have to be utilized. Guidelines for the selection of the individual components are provided herein. The Antimicrobial Active The antimicrobial cleansing composition of the antimicrobial wipes of the present invention comprises from about 0.001% to about 5%, preferably from about 0.05% to about 1%, more preferably from about 0.05% to about 0.5% and more preferably from about 0.1% to about 0.25%, by weight of the antimicrobial cleansing composition, of an antimicrobial active. The exact amount of antibacterial active to be used in the compositions will depend on the particular active utilized since actives vary in potency. Non-cationic actives are required in order to avoid interaction with the anionic surfactants of the invention. Given below are examples of non-cationic antimicrobial agents which are useful in the present invention . Pyrithiones, especially the zinc complex (ZPT) Octopirox® Dimethyldimethylol Hydantoin (Glydant®) Methylchloroisothiazolinone/methylisothiazolinone (Kathon CG®) Sodium Sulfite Sodium Bisulfite Imidazolidinyl Urea (Germall 115®) Diazolidinyl Urea (Germall II®) Benzyl Alcohol 2-Bromo-2-nitro propane- 1 ,3-diol (Bronopol®) Formalin (formaldehyde) Iodopropenyl Butylcarbamate (Polyphase PI 00®) Chloroacetamide Methanamine Methyldibromonitrile Glutaronitrile (l,2-Dibromo-2,4-dicyanobutane or Tektamer®) Glutaraldehyde 5-bromo-5-nitro- 1 ,3-dioxane (Bronidox®) Phenethyl Alcohol o-Phenylphenol/sodium o-phenylphenol Sodium Hydroxymethylglycinate (Suttocide A®) Polymethoxy Bicyclic Oxazolidine (Nuosept C®) Dimethoxane Thimersal Dichlorobenzyl Alcohol Captan Chlorphenenesin Dichlorophene Chlorbutanol Glyceryl Laurate Halogenated Diphenyl Ethers 2,4,4'-trichloro-2'-hydroxy-diphenyl ether (Triclosan® or TCS) 2,2'-dihydroxy-5 ,5 '-dibro mo-diphenyl ether Phenolic Compounds Phenol 2-Methyl Phenol 3-Methyl Phenol 4-Methyl Phenol 4-Ethyl Phenol 2,4-Dimethyl Phenol 2,5-Dimethyl Phenol 3, 4-Dimethyl Phenol 2,6-Dimethyl Phenol 4-n-Propyl Phenol 4-n-Butyl Phenol 4-n-Amyl Phenol 4-tert-Amyl Phenol 4-n-Hexyl Phenol 4-n-Heptyl Phenol Mono- and Poly-Alkyl and Aromatic Halophenols p-Chlorophenol Methyl p-Chlorophenol Ethyl p-Chlorophenol n-Propyl p-Chlorophenol n-Butyl p-Chlorophenol n-Amyl p-Chlorophenol sec-Amyl p-Chlorophenol n-Hexyl p-Chlorophenol Cyclohexyl p-Chlorophenol n-Heptyl p-Chlorophenol n-Octyl p-Chlorophenol o-Chlorophenol Methyl o-Chlorophenol Ethyl o-Chlorophenol n-Propyl o-Chlorophenol n-Butyl o-Chlorophenol n-Amyl o-Chlorophenol tert-Amyl o-Chlorophenol n-Hexyl o-Chlorophenol n-Heptyl o-Chlorophenol o-Benzyl p-Chlorophenol o-Benxyl-m-methyl p-Chlorophenol o-Benzyl-m, m-dimethyl p-Chlorophenol o-Phenylethyl p-Chlorophenol o-Phenylethyl-m-methyl p-Chlorophenol 3 -Methyl p-Chlorophenol 3,5-Dimethyl p-Chlorophenol 6-Ethyl-3-methyl p-Chlorophenol 6-n-Propyl-3-methyl p-Chlorophenol 6-iso-Propyl-3-methyl p-Chlorophenol 2-Ethyl-3,5-dimethyl p-Chlorophenol 6-sec-Butyl-3-methyl p-Chlorophenol 2-iso-Propyl-3 , 5 -dimethyl p-Chlorophenol 6-Diethylmethyl-3-methyl p-Chlorophenol 6-iso-Propyl-2-ethyl-3-methyl p-Chlorophenol 2-sec-Amyl-3,5-dimethyl p-Chlorophenol 2-Diethylmethyl-3,5-dimethyl p-Chlorophenol 6-sec-Octyl-3 -methyl p-Chlorophenol p-Chloro-m-cresol p-Bromophenol Methyl p-Bromophenol Ethyl p-Bromophenol n-Propyl p-Bromophenol n-Butyl p-Bromophenol n-Amyl p-Bromophenol sec-Amyl p-Bromophenol n-Hexyl p-Bromophenol Cyclohexyl p-Bromophenol o-Bromophenol tert-Amyl o-Bromophenol n-Hexyl o-Bromophenol n-Propyl-m,m-Dimethyl o-Bromophenol 2-Phenyl Phenol 4-Chloro-2 -methyl phenol 4-Chloro-3 -methyl phenol 4-Chloro-3, 5 -dimethyl phenol 2,4-Dichloro-3,5-dimethylphenol 3 ,4,5 ,6-Terabromo-2-methylphenol 5-Methyl-2-pentylphenol 4-Isopropyl-3-methylphenol Para-chloro-meta-xylenol (PCMX) Chlorothymol Phenoxyethanol Phenoxyisopropanol 5-Chloro-2-hydroxydiphenylmethane Resorcinol and its Derivatives Resorcinol Methyl Resorcinol O 00/61105 10 Ethyl Resorcinol n-Propyl Resorcinol n-Butyl Resorcinol n-Amyl Resorcinol n-Hexyl Resorcinol n-Heptyl Resorcinol n-Octyl Resorcinol n-Nonyl Resorcinol Phenyl Resorcinol Benzyl Resorcinol Phenylethyl Resorcinol Phenylpropyl Resorcinol p-Chlorobenzyl Resorcinol 5-Chloro 2,4-Dιhydroxydιphenyl Methane 4'-Chloro 2,4-Dιhydroxydιphenyl Methane 5-Bromo 2,4-Dιhydroxydφhenyl Methane 4' -Bromo 2,4-Dιhydroxydιphenyl Methane Bisphenohc Compounds 2,2'-Methylene bis (4-chlorophenol) 2,2'-Methylene bis (3,4,6-tπchlorophenol) 2,2'-Methylene bis (4-chloro-6-bromophenol) bis (2-hydroxy-3,5-dιchlorophenyl) sulphide bis (2-hydroxy-5-chlorobenzyl)sulρhιde Benzoic Esters (Parabens) Methylparaben Propylparaben Butylparaben Ethylparaben Isopropylparaben Isobutylparaben Benzylparaben Sodium Methylparaben Sodium Propylparaben Halogenated Carbamlides 3,4,4'-Tπchlorocarbanιhdes (Tnclocarban®or TCC) 3-Tnfluoromethyl-4,4'-dιchlorocarbanιhde 3,3',4-Tnchlorocarbanιlιde Another class of antibactenal agents, which are useful in the present invention, are the so- called "natural" antibacterial actives, referred to as natural essential oils These actives denve their names from their natural occurrence m plants Typical natural essential oil antibactenal actives include oils of anise, lemon, orange, rosemary, wmtergreen, thyme, lavender, cloves, hops, tea tree, citronella, wheat, barley, lemongrass, cedar leaf, cedarwood, cinnamon, fleagrass, geranium, sandalwood, violet, cranberry, eucalyptus, vervain, peppermint, gum benzoin, basil, fennel, fir, balsam, menthol, ocmea origanum, Hydastis carradensis. Berberidaceae daceae, Ratanhiae and Curcuma longa Also included this class of natural essential oils are the key chemical components of the plant oils which have been found to provide the antimicrobial benefit These chemicals include, but are not limited to anethol. catechole, camphene, carvacol, eugenol, eucalyptol, feruhc acid, famesol, hmokitiol, tropolone, hmonene, menthol, methyl sahcylate, thymol, terpmeol, verbenone, berbenne, ratanhiae extract, caryophellene oxide, citronelhc acid, curcumin, nerohdol and geraniol. Additional active agents are antibacterial metal salts. This class generally includes salts of metals in groups 3b- 7b, 8 and 3a-5a. Specifically are the salts of aluminum, zirconium, zmc, silver, gold, copper, lanthanum, tin, mercury, bismuth, selenium, strontium, scandium, yttrium, cenum, praseodymiun, neodymium, promethum, samanum, europium, gadolinium, terbium, dysprosium, holmium, erbium, thulium, ytterbium, lutetium and mixtures thereof. Preferred antimicrobial agents for use herein are the broad spectrum actives selected from the group consisting of Tnclosan®, Tnclocarban®, Octopirox®, PCMX, ZPT, natural essential oils and their key ingredients, and mixtures thereof. The most preferred antimicrobial active for use in the present invention is Tnclosan®. Anionic Surfactant The antimicrobial cleansing compositions of the present invention compnse from about 0.05% to about 10%, preferably from about 0.1 to about 2%, and more preferably from about 0.2% to about 1%, by weight of the cleansing composition, of an anionic surfactant. Without being limited by theory, it is believed that the anionic surfactant disrupts the hpid in the cell membrane of the bactena. The particular acid used herein reduces the negative charges on the cell wall of the bactena, crosses through the cell membrane, weakened by the surfactant, and acidifies the cytoplasm of the bactena. The antimicrobial active can then pass more easily through the weakened cell wall, and more efficiently poison the bactena. Nonlimitmg examples of anionic lathenng surfactants useful the compositions of the present invention are disclosed m McCutcheon's, Detergents and Emulsifiers. North American edition (1990), published by The Manufactunng Confectioner Publishing Co.; McCutcheon's, Functional Matenals. North American Edition (1992); and U.S. Patent No. 3,929,678, to Laughlm et al., issued December 30, 1975, all of which are incorporated by reference A wide vanety of anionic surfactants are potentially useful herein. Nonlimitmg examples of anionic lathenng surfactants include those selected from the group consisting of alkyl and alkyl ether sulfates, sulfated monoglycendes, sulfonated olefins, alkyl aryl sulfonates, pnmary or secondary alkane sulfonates, alkyl sulfosuccmates, acyl taurates, acyl lsethionates, alky] gly eery 1 ether sulfonate, sulfonated methyl esters, sulfonated fatty acids, alkyl phosphates, acyl glutamates, acyl sarcosmates, alkyl sulfoacetates, acylated peptides, alkyl ether carboxylates, acyl lactylates, anionic fluorosurfactants, and mixtures thereof Mixtures of anionic surfactants can be used effectively in the present invention A onic surfactants for use in the cleansing compositions include alkyl and alkyl ether sulfates These matenals have the respective formulae R 1 0-S03M and R^C^H-jO^-O- 00/61105 12 SO3M, wherein R' is a saturated or unsaturated. branched or unbranched alkyl group from about 8 to about 24 carbon atoms, x is 1 to 10, and M is a water-soluble cation such as ammonium, sodium, potassium, magnesium, tnethanolamme, diethanolamme and monoethanolamme. The alkyl sulfates are typically made by the sulfation of monohydπc alcohols (having from about 8 to about 24 carbon atoms) using sulfur tnoxide or other known sulfation technique. The alkyl ether sulfates are typically made as condensation products of ethylene oxide and monohydπc alcohols (having from about 8 to about 24 carbon atoms) and then sulfated. These alcohols can be denved from fats, e.g., coconut oil or tallow, or can be synthetic. Specific examples of alkyl sulfates which may be used the cleanser compositions are sodium, ammonium, potassium, magnesium, or TEA salts of lauryl or mynstyl sulfate. Examples of alkyl ether sulfates which may be used include ammonium, sodium, magnesium, or TEA laureth-3 sulfate. Another suitable class of anionic surfactants are the sulfated monoglyceπdes of the form R 1 CO-0-CH2-C(OH)H-CH2-0-S03M, wherein R 1 is a saturated or unsaturated, branched or unbranched alkyl group from about 8 to about 24 carbon atoms, and M is a water-soluble cation such as ammonium, sodium, potassium, magnesium, tnethanolamme, diethanolamme and monoethanolamme. These are typically made by the reaction of glycenn with fatty acids (having from about 8 to about 24 carbon atoms) to form a monoglyceπde and the subsequent sulfation of this monoglyceπde with sulfur tnoxide. An example of a sulfated monoglyceπde is sodium cocomonoglycende sulfate. Other suitable anionic surfactants include olefin sulfonates of the form RISO3M, wherein R! IS a mono-olefin having from about 12 to about 24 carbon atoms, and M is a water-soluble cation such as ammonium, sodium, potassium, magnesium, tnethanolamme, diethanolamme and monoethanolamme. These compounds can be produced by the sulfonation of alpha olefms by means of uncomplexed sulfur tnoxide, followed by neutralization of the acid reaction mixture in conditions such that any sultones which have been formed in the reaction are hydrolyzed to give the corresponding hydroxyalkanesulfonate. An example of a sulfonated olefin is sodium C14/C16 alpha olefin sulfonate. Other suitable anionic surfactants are the linear alkylbenzene sulfonates of the form R'" C6H4-SO3M, wherein R is a saturated or unsaturated, branched or unbranched alkyl group from about 8 to about 24 carbon atoms, and M is a water-soluble cation such as ammonium, sodium, potassium, magnesium, tnethanolamme, diethanolamme and monoethanolamme. These are formed by the sulfonation of linear alkyl benzene with sulfur tnoxide An example of this anionic surfactant is sodium dodecylbenzene sulfonate Still other anionic surfactants suitable for this cleansing composition include the primary or secondary alkane sulfonates of the form RISO3M, wherein R^ is a saturated or unsaturated, branched or unbranched alkyl chain from about 8 to about 24 carbon atoms, and M is a water- soluble cation such as ammonium, sodium, potassium, magnesium, tnethanolamme, diethanolamme and monoethanolamme These are commonly formed by the sulfonation of paraffins using sulfur dioxide in the presence of chlorine and ultraviolet light or another known sulfonation method. The sulfonation can occur in either the secondary or pnmary positions of the alkyl chain. An example of an alkane sulfonate useful herein is alkali metal or ammonium Ci 3-C17 paraffin sulfonates. Still other suitable anionic surfactants are the alkyl sulfosuccinates, which include disodium N-octadecylsulfosuccinamate; diammonium lauryl sulfosuccmate; tetrasodium N-( 1 ,2- dιcarboxyethyl)-N-octadecylsulfosuccmate; diamyl ester of sodium sulfosuccimc acid; dihexyl ester of sodium sulfosuccimc acid; and dioctyl esters of sodium sulfosuccimc acid. Also useful are taurates which are based on taunne, which is also known as 2- ammoethanesulfonic acid Examples of taurates include N-alkyltaunnes such as the one prepared by reacting dodecylamme with sodium lsethionate according to the teaching of U.S Patent 2,658,072 which is incorporated herein by reference m its entirety. Other examples based of taunne include the acyl taunnes formed by the reaction of n-methyl taunne with fatty acids (having from about 8 to about 24 carbon atoms) Another class of anionic surfactants suitable for use in the cleansing composition are the acyl lsethionates. The acyl lsethionates typically have the formula RICO-O-CL-2CH2SO3M wherein R* is a saturated or unsaturated, branched or unbranched alkyl group having from about 10 to about 30 carbon atoms, and M is a cation These are typically formed by the reaction of fatty acids (having from about 8 to about 30 carbon atoms) with an alkali metal lsethionate. Nonlimitmg examples of these acyl lsethionates include ammonium cocoyl lsethionate, sodium cocoyl lsethionate, sodium lauroyl lsethionate. and mixtures thereof Still other suitable anionic surfactants are the alkyl glyceryl ether sulfonates of the form R OCH2-C(OH)H-CH2-Sθ3M, wherein R^ is a saturated or unsaturated, branched or unbranched alkyl group from about 8 to about 24 carbon atoms, and M is a water-soluble cation such as ammonium, sodium, potassium, magnesium, tnethanolamme, diethanolamme and monoethanolamme. These can be formed by the reaction of epichlorohydnn and sodium bisulfite with fatty alcohols (having from about 8 to about 24 carbon atoms) or other known methods One example is sodium cocoglyceryl ether sulfonate Other suitable anionic surfactants include the sulfonated fatty acids of the form R*- CH(S0 4 )-COOH and sulfonated methyl esters of the from R 1 -CH(S0 4 )-CO-0-CH 3 , where R 1 is a saturated or unsaturated, branched or unbranched alkyl group from about 8 to about 24 carbon atoms These can be formed by the sulfonation of fatty acids or alkyl methyl esters (having from about 8 to about 24 carbon atoms) with sulfur tnoxide or by another known sulfonation technique. Examples include alpha sulphonated coconut fatty acid and lauryl methyl ester. Other anionic matenals include phosphates such as monoalkyl, dialkyl, and tnalkylphosphate salts formed by the reaction of phosphorous pentoxide with monohydπc branched or unbranched alcohols having from about 8 to about 24 carbon atoms. These could also be formed by other known phosphation methods. An example from this class of surfactants is sodium mono or dilaurylphosphate. Other a onic matenals include acyl glutamates corresponding to the formula R^CO- N(COOH)-CH2CH2-Cθ2M wherein R* is a saturated or unsaturated, branched or unbranched alkyl or alkenyl group of about 8 to about 24 carbon atoms, and M is a water-soluble cation. Nonlimitmg examples of which include sodium lauroyl glutamate and sodium cocoyl glutamate. Other anionic matenals include alkanoyl sarcosmates corresponding to the formula R*CON(CH3)-CH2CH2-C02M wherein R^ is a saturated or unsaturated, branched or unbranched alkyl or alkenyl group of about 10 to about 20 carbon atoms, and M is a water- soluble cation. Nonlimitmg examples of which include sodium lauroyl sarcosmate, sodium cocoyl sarcosmate, and ammonium lauroyl sarcosmate Other anionic materials include alkyl ether carboxylates corresponding to the formula R*" (OCH2CH2) χ -OCH2-Cθ2M wherein R* is a saturated or unsaturated, branched or unbranched alkyl or alkenyl group of about 8 to about 24 carbon atoms, x is 1 to 10, and M is a water-soluble cation. Nonlimitmg examples of which include sodium laureth carboxylate. Other anionic matenals include acyl lactylates corresponding to the formula R*CO-[0- CH(CH3)-CO] x -C02M wherein R' is a saturated or unsaturated, branched or unbranched alkyl or alkenyl group of about 8 to about 24 carbon atoms, x is 3, and M is a water-soluble cation. Nonlimitmg examples of which include sodium cocoyl lactylate. Other anionic materials include the carboxylates, nonlimitmg examples of which include sodium lauroyl carboxylate, sodium cocoyl carboxylate, and ammonium lauroyl carboxylate. Anionic flourosurfactants can also be used. Any counter cation, M, can be used on the anionic surfactant. Preferably the counter cation is selected from the group consisting of sodium, potassium, ammonium, monoethanolamme, diethanolamme, and tnethanolamme. More preferably the counter cation is ammonium Two factors must be taken into account when selecting the surfactant or surfactants to be employed in the antibacterial cleansing compositions of the antimicrobial wipes herein 1) the activity of the surfactant molecule at the cell membrane of the bacteria: and 2) the mildness of the surfactant insofar as it affects the Mildness Index (hereinafter described) for the antibacterial composition Biological Activity/Mildness of Surfactant In general, the higher the biological activity of the surfactant, the more residual effectiveness is provided by the composition compnsmg the surfactant. Typically, however, the biological activity of a surfactant and the mildness of a surfactant are inversely proportional; the higher the biological activity of the surfactant, the harsher the surfactant and the lower the biological activity of the surfactant, the milder the surfactant. Whether a biologically active, but harsh surfactant or a mild, but biologically inactive surfactant is desired will, of course, depend on (or influence) the selection of the other components. The biological activity/mildness of a pure surfactant can measured directly via a Microtox Response Test hereinafter descnbed m the Analytical Methods section and can be reported as a Microtox Response Index. By "pure surfactant" it is meant a chemical composition consisting essentially of a single surfactant entity, wherein the entity has essentially one chain length, head group and salt counter ion. From a standpoint of high biological activity, preferred anionic surfactants of the antimicrobial cleansing compositions of the present invention have a Microtox Response Index of less that about 150, more preferably less than about 100 and most preferably less than about 50. From a standpoint of mildness, preferred amonic surfactants of the antimicrobial cleansing compositions of the present invention have a Microtox Response Index of greater than about 25, more preferably greater than about 50 and most preferably greater than about 100. Surfactants with a Microtox Response Index ranging from about 25 to about 150 are typically moderately biologically active and moderately mild. For surfactant compositions which are mixtures of surfactants rather than pure surfactants (this includes "commercial grade" surfactants which typically comprise mixtures of entities with different chain lenghts and potentially have higer levels of impurities), the Microtox Response Index for any individual surfactant component is not a reliable measurement of biological activity or mildness. In the case of mixtures, the Microtox Index of each individual component can be determined and the weighted average used as the Index for the mixture if all the individual components of the mixture are known If the individual components of a mixture are not known, then the pnmary head group and chain lengths of the surfactant mixture are better indicators of biological activity/mildness Anionic surfactants or mixtures of surfactants with a chain length primarily m the range of from about 8 to about 24 carbon atoms, preferably primarily from about 10 to about 18 carbon atoms and most preferably primarily from about 12 to about 16 carbon atoms are preferred from the standpoint of high biological activity As used herein "pnmaπly" means at least about 50% From a standpoint of mildness, it is preferable to minimize C12 From the standpoint of biological activity, it is preferred that the head group of the anionic surfactant be less than about 15 Angstroms, preferably less than about 10 Angstoms, and more preferably less than about 7 Angstoms. The "head group" is defined as the hydrophilic portion (non-hydrocarbon) of the amonic surfactant, measured from the first polar atom to the end of the molecule. The head group size is estimated from the Van der Waals radius of the atoms and the configuration of the surfactant molecule. Head groups with sizes less than about 7 Angstroms include sulfates, sulfonates, and phosphates. From the standpoint of mildness, it is preferred that the head group size is greater than about 7 Angstoms, and preferably greater than about 10 Angstoms. Head groups with sizes greater than about 10 Angstroms include ethoxylated sulfates, glyceryl ether sulfonates, and lsethionates. It is believed that as the head group size increases, more steanc hindrance at the cell wall prevents disruption by the surfactant and, thus, biological activity is decreased and mildness is increased. The mildness of a surfactant or mixture of surfactants can also be determined by a number of other known, conventional methods for measuring surfactant mildness. For example, the Bamer Destruction Test set forth in T. J. Franz, J. Invest. Dermatol.. 1975, 64, pp. 190-195 and in U.S. Patent 4,673,525 to Small et al; issued June 16, 1987, both of which are herein incorporated by reference, is a way of measunng mildness of surfactants. In general, the milder the surfactant, the less skm barner that is destroyed m the barrier destruction test. Skin barner destruction is measured by relative amount of radiolabeled water which passes from the test solution through the sk epidermis into the physiological buffer contained m the diffusate chamber. Surfactants having a Relative Skm Bamer Penetration Value of as close to zero as possible up to about 75 are considered mild for purposes herein. Surfactants having a Relative Skin Barrier Penetration Value of greater than about 75 are considered harsh for purposes herein. In order for the antimicrobial composition of the antimicrobial wipes herein to be effective, both the biological activity of the surfactant and the mildness of the surfactant and acid employed m the composition must be taken into account. For example, ammonium lauryl sulfate, ALS, is very biologically active (Microtox Index = 1.0). Compositions compnsmg ALS are capable of providing very effective residual antibacterial effectiveness due to its activity, even with lower levels of antibactenal active and proton donating agent. However, compositions containing ALS may require the addition of co- surfactants or polymers, descnbed herein in the Optional Ingredient Section, to achieve most preferred mildness levels for the present invention A selection of ammonium laureth-3 sulfate (Microtox = 120) as a surfactant will result in compositions which are very mild, but which would require higher levels of proton donating agent and antimicrobial active in order to achieve the residual effectiveness of the present invention Paraffin sulfonate, a commercial grade surfactant sold under the name Hastapur SAS® from Hoechst Celanese, with a small head group and average chain length of 15.5 is a relatively 00/61105 17 active surfactant Compositions comprising lower levels of active and acid can be used with higher levels of paraffin sulfonate, where the surfactant provides a larger component of residual effectiveness. Alternately, compositions comprising lower levels of paraffin sulfonate can be combined with even higher levels of active to achieve a mild and effective composition. Nonlimitmg examples of preferred anionic surfactants useful herein include those selected from the group consisting of sodium and ammonium alkyl sulfates and ether sulfates having chain lengths of predominantly 12 and 14 carbon atoms, olefin sulfates having chain lengths of predominantly 14 and 16 carbon atoms, and paraffin sulfonates having chain lengths of from 13 to 17 carbon atoms, and mixtures thereof. Especially preferred for use herein is ammonium and sodium lauryl sulfate, ammonium and sodium mynstyl sulfate, ammonium and sodium laureth- 1 to laureth-4 sulfate, C14-C16 olefin sulfonates, C13-C17 paraffin sulfonates, and mixtures thereof. Non-anionic surfactants of the group consisting of catiomc surfactants, amphotenc surfactants and mixtures thereof, have been found to actually inhibit residual effectiveness benefits. It is believed that these surfactants interfere with the anionic surfactant disruption of the pid in the cell membrane. The ratio of the amount of these non-anionic surfactants to the amount of anionic surfactant should be less than about 1 : 1, preferably less than about 1 :2, and more preferably less than about 1 :4. The antimicrobial cleansing compositions of the present invention preferably do not comprise hydrotropic sulfonates, particularly salts of terpenoids, or mono- or bmuclear aromatic compounds such as sulfonates of camphor, toluene, xylene, cumene and naphthene. Proton Donating Agent The antimicrobial cleansing compositions of the present invention compnse from about 0.1% to about 10%, preferably from about 0.5% to about 8%, more preferably from about 1% to about 5%, based on the weight of the personal cleansing composition, of a proton donating agent By "proton donating agent" it is meant any acid compound or mixture thereof, which results m undissociated acid on the skm after use. Proton donating agents can be organic acids, including polymeric acids, mineral acids or mixtures thereof. O 00/61105 Organic Acids Proton donating agents which are organic acids remain at least partially undissociated m the neat composition and remain so when the compositions are diluted dunng washing and nnsmg. The organic acid proton donating agent must have at least one pKa value below 5.5. These organic proton donating agents can be added directly to the composition in the acid form or can be formed by adding the conjugate base of the desired acid and a sufficient amount of a separate acid strong enough to form the undissociated acid from the base. Buffenng Capacity Preferred organic proton donating agents are selected and formulated based on their buffer capacity and pKa. Buffer capacity is defined as the amount of protons (weight %) available m the formulation at the product pH for those acid groups with pKa's less than about 6.0. Buffer capacity can be either calculated using pKa's, pH, and the concentrations of the acids and conjugate bases, ignonng any pKa greater than 6.0, or it can be determined expenmentally through a simple acid-base titration using sodium hydroxide or potassium hydroxide using an endpomt of pH equals 6.0. Preferred organic proton donating agents of the antibactenal cleansing composition herein have a buffer capacity of greater than about 0.005%, more preferably greater than about 0.01%, even more preferably greater than about 0.02%, and most preferably greater than about 0.04%. Mineral Acids Proton donating agents which are mineral acids will not remain undissociated m the neat composition and when the compositions are diluted during washing and nnsmg. Despite this, it has been found that mineral acids can be effective proton donating agents for use herein Without being limited by theory, it is believed that the strong mineral acid, acidify the carboxyhc and phosphatidyl groups in proteins of the skm cells, thereby providing in-situ undissociated acid These proton donating agents can only be added directly to the composition m the acid form. ES It is cntical to achieving the benefits of the invention that the undissociated acid from the proton donating agent (deposited or formed in-situ) remain on the skm m the protonated form Therefore, the pH of the antimicrobial cleansing compositions of the present invention must be adjusted to a sufficiently low level in order to either form or deposit substantial undissociated acid on the skin. The pH of the compositions should be adjusted and preferably buffered to range from about 3.0 to about 6.0, preferably from about 3 0 to about 5.0 and more preferably from about 3.5 to about 4.5 A non-exclusive list of examples of organic acids which can be used as the proton donating agent are adrpic acid, tartaπc acid, citric acid, maleic acid, malic acid, succmic acid, glycohc acid, glutanc acid, benzoic acid, malonic acid, salicylic acid, gluconic acid, gluconolactone (especially glucono-delta-lactone), 2-pyrrolιdone-5 carboxyhc acid, polyacryhc acid, salts thereof, isomers thereof, and mixtures thereof. A non-exclusive list of examples of mineral acid for use herein are hydrochloric, phosphoric, sulfunc and mixtures thereof. Preferred for use herein are 2-pyrrohdone-5 carboxyhc acid, gluconolactone, isomers thereof, and mixtures thereof. Water The antimicrobial cleansing compositions of the present invention compnse from about 3% to about 98.899%, preferably from about 5% to about 98%, more preferably from about 10% to about 97.5%, and most preferably from about 38% to about 95.99% water. Preferable Optional Ingredients Mildness Enhancers In order to achieve the mildness required of the present invention, optional ingredients to enhance the mildness to the skm can be added. These ingredients include catiomc and noniomc polymers, co-surfactants, moistunzers and mixtures thereof. Polymers useful herein include polyethylene glycols, polypropylene glycols, hydrolyzed silk proteins, hydrolyzed milk proteins, hydrolyzed keratm proteins, guar hydroxypropyltnmomum chloride, polyquats, si cone polymers and mixtures thereof. When used, the mildness enhancing polymers comprise from about 0.1% to about 1%, preferably from about 0.2% to about 1.0%, and more preferably from about 0.2% to about 0.6%, by weight of the antimicrobial cleasnmg composition, of the composition. Co-surfactants useful herein include noniomc surfactants such as the Genapol® 24 senes of ethoxylated alcohols, POE(20) sorbitan monooleate (Tween® 80), polyethylene glycol cocoate and Pluromc® propylene oxide/ethylene oxide block polymers, and amphotenc surfactants such as alkyl betaines, alkyl sultames, alkyl amphoacetates, alkyl amphodiacetates, alkyl amphopropionates, and alkyl amphodipropionates. When used, the mildness enhancing cosurfactants comprise from about 20% to about 70%, preferably from about 20% to about 50%, by weight of the anionic surfactant, of the cleansing composition Another group of mildness enhancers are hpid skm moisturizing agents which provide a moistunzing benefit to the user of the cleansing wipe when the hpophilic skm moisturizing agent is deposited to the user's skm When used in the antimicrobial personal cleansing compositions herein, hpophilic skm moisturizing agents are used, they are employed at a level of about 0.1% to about 30%, preferably from about 0.2% to about 10%, most preferably from about 0.5% to about 5% by weight of the composition In some cases, the hpophilic skin moisturizing agent can desirably be defined in terms of its solubility parameter, as defined by Vaughan m Cosmetics and Toiletries. Vol. 103, p. 47-69, October 1988. A hpophilic skin moistunzing agent having a Vaughan Solubility Parameter O 00/61105 20 (VSP) from 5 to 10, preferably from 5.5 to 9 is suitable for use in the antimicrobial cleansing compositions herein. A wide vanety of hpid type materials and mixtures of materials are suitable for use m the antimicrobial cleansing compositions of the present invention Preferably, the hpophilic skm conditioning agent is selected from the group consisting of hydrocarbon oils and waxes, si cones, fatty acid derivatives, cholesterol, cholesterol denvatives, di and tπ-glyceπdes, vegetable oils, vegetable oil denvatives, liquid nondigestible oils such as those descnbed in U.S. Patents 3,600,186 to Mattson; Issued August 17, 1971 and 4,005,195 and 4,005,196 to Jandacek et al; both issued January 25, 1977, all of which are herein incorporated by reference, or blends of liquid digestible or nondigestible oils with solid polyol polyesters such as those descnbed m U.S. Patent 4,797,300 to Jandacek; issued January 10, 1989; U.S Patents 5,306,514, 5,306,516 and 5,306,515 to Letton; all issued April 26, 1994, all of which are herein incorporated by reference, and acetoglycende esters, alkyl esters, alkenyl esters, lanolin and its denvatives, milk tn-glycendes, wax esters, beeswax denvatives, sterols, phosphohpids and mixtures thereof. Fatty acids, fatty acid soaps and water soluble polyols are specifically excluded from our definition of a hpophilic skin moistunzing agent. Hydrocarbon oils and waxes: Some examples are petrolatum, mineral oil microcrystalhne waxes, polyalkenes (e.g. hydrogenated and nonhydrogenated polybutene and polydecene), paraffins, cerasm, ozokerite, polyethylene and perhydrosqualene Blends of petrolatum and hydrogenated and nonhydrogenated high molecular weight polybutenes wherein the ratio of petrolatum to polybutene ranges from about 90: 10 to about 40:60 are also suitable for use as the hpid skin moistunzing agent m the compositions herein. Sihcone Oils: Some examples are dimethicone copolyol, dimethylpolysiloxane, diethylpolysiloxane, high molecular weight dimethicone, mixed C1-C30 alkyl polysiloxane, phenyl dimethicone, dimethiconol, and mixtures thereof. More preferred are non-volatile sihcones selected from dimethicone, dimethiconol, mixed C1-C30 alkyl polysiloxane, and mixtures thereof. Nonlimitmg examples of sihcones useful herein are described in U.S. Patent No. 5,011,681, to Ciotti et al., issued April 30, 1991, which is incorporated by reference. Di- and tn-glvcendes: Some examples are castor oil, soy bean oil, denvatized soybean oils such as maleated soy bean oil, safflower oil, cotton seed oil, com oil, walnut oil, peanut oil, olive oil, cod liver oil, almond oil, avocado oil, palm oil and sesame oil, vegetable oils and vegetable oil derivatives; coconut oil and denvatized coconut oil, cottonseed oil and denvatized cottonseed oil, jojoba oil, cocoa butter, and the like Acetoglvceπde esters are used and an example is acetylated monoglycendes Lanolin and its derivatives are preferred and some examples are lanolm, lanolm oil, lanolin wax, lanolm alcohols, lanolm fatty acids, isopropyl lanolate, acetylated lanolm, acetylated lanolm alcohols, lanolm alcohol hnoleate, lanolm alcohol nconoleate. It is most preferred when at least 75 % of the hpophilic skm conditioning agent is compnsed of lipids selected from the group consisting: petrolatum, blends of petrolatum and high molecular weight polybutene, mineral oil, liquid nondigestible oils (e.g. liquid cottonseed sucrose octaesters) or blends of liquid digestible or nondigestible oils with solid polyol polyesters (e.g. sucrose octaesters prepared from C22 fatty acids) wherein the ratio of liquid digestible or nondigestible oil to solid polyol polyester ranges from about 96:4 to about 80:20, hydrogenated or nonhydrogenated polybutene, microcrystalhne wax, polyalkene, paraffin, cerasm, ozokente, polyethylene, perhydrosqualene; dimethicones, alkyl siloxane, polymethylsiloxane, methylphenylpolysiloxane and mixtures thereof. When as blend of petrolatum and other lipids is used, the ratio of petrolatum to the other selected lipids (hydrogenated or unhydrogenated polybutene or polydecene or mineral oil) is preferably from about 10:1 to about 1 :2, more preferably from about 5: 1 to about 1 : 1. Stabilizers When a hpophilic skm moistunzing agent is employed as the mildness enhancer in the antimicrobial compositions herein, a stabilizer may also be included at a level ranging from about 0.1% to about 10%, preferably from about 0.1 % to about 8%, more preferably from about 0.1% to about 5% by weight of the antimicrobial cleansing composition. The stabilizer is used to form a crystalline stabilizing network in the liquid cleansing composition that prevents the hpophilic skm moistunzer agent droplets from coalescing and phase splitting m the product. The network exhibits time dependent recovery of viscosity after sheanng (e.g., thixotropy). The stabilizers used herein are not surfactants. The stabilizers provide improved shelf and stress stability. Some preferred hydroxyl-contaming stabilizers include 12-hydroxysteanc acid, 9,10-dιhydroxysteanc acid, tπ-9, 10-dιhydroxysteaπn and tπ-12-hydroxysteaπn (hydrogenated castor oil is mostly tn-12-hydroxysteaπn). Tn-12-hydroxysteann is most preferred for use in the compositions herein When these crystalline, hydroxyl-contammg stabilizers are utilized in the cleansing compositions herein, they are typically present at from about 0 1% to 10%, preferably from 0.1% to 8%. more preferably from 0 1% to about 5% of the antimicrobial cleansing compositions. The stabilizer is insoluble in water under ambient to near ambient conditions Alternatively, the stabilizer employed in the cleansing compositions herein can comprise a polymeric thickener When polymeric thickeners as the stabilizer in the cleansing compositions herein, they are typically included in an amount ranging from about 0.01% to about 5%, preferably from about 0.3% to about 3%, by weight of the composition. The polymenc thickener is preferably an anionic, noniomc, cationic or hydrophobically modifier polymer selected from the group consisting of cationic polysaccharides of the cationic guar gum class with molecular weights of 1,000 to 3,000,000, amonic, cationic, and noniomc homopolymers denved from acrylic and or methacryhc acid, a onic, cationic and noniomc cellulose resins, cationic copolymers of dimethyldialkylammonium chloride, and acrylic acid, cationic homopolymers of dimethylalkylammomum chlonde, cationic polyalklene, and ethoxypolyalkylene lmines, polyethylene glycol of molecular weight from 100,000 to 4,000,000, and mixtures thereof. Preferably, the polymer is selected from the group consisting of sodium polyacrylate, hydroxy ethyl cellulose, cetyl hydroxy ethyl cellulose, and Polyquaternium 10. Alternatively, the stabilizer employed in the cleansing compositions herein can compnse C10-C22 ethylene glycol fatty acid esters. C10-C22 ethylene glycol fatty acid esters can also desirably be employed in combination with the polymenc thickeners hereinbefore descnbed. The ester is preferably a diester, more preferably a C14-C18 diester, most preferably ethylene glycol distearate. When C10-C22 ethylene glycol fatty acid esters are utilized as the stabilizer m the personal cleansing compositions herein, they are typically present at from about 3% to about 10%, preferably from about 5% to about 8%, more preferably from about 6% to about 8% of the personal cleansing compositions. Another class of stabilizer which can be employed in the antimicrobial cleansing compositions of the present invention compnses dispersed amorphous silica selected from the group consisting of fumed silica and precipitated silica and mixtures thereof. As used herein the term "dispersed amorphous silica" refers to small, finely divided non-crystallme silica having a mean agglomerate particle size of less than about 100 microns. Fumed silica, which is also known as arced silica, is produced by the vapor phase hydrolysis of silicon tetrachlonde m a hydrogen oxygen flame. It is believed that the combustion process creates sihcone dioxide molecules which condense to form particles. The particles collide, attach and smter together. The result of this process is a three dimensional branched chain aggregate. Once the aggregate cools below the fusion point of silica, which is about 1710° C, further collisions result in mechanical entanglement of the chains to form agglomerates Precipitated silicas and silica gels are generally made in aqueous solution. See, Cabot Technical Data Pamphlet TD-100 entitled "CAB-O-SIL® Untreated Fumed Silica Properties and Functions". October 1993, and Cabot Technical Dat Pamphlet TD- 04 entitled "CAB-O-SIL® Fumed Silica in Cosmetic and Personal Care Products", March 1992, both of which are herein incorporated by reference. The fumed silica preferably has a mean agglomerate particle size ranging from about 0.1 microns to about 100 microns, preferably from about 1 micron to about 50 microns, and more preferably from about 10 microns to about 30 microns. The agglomerates are composed of aggregates which have a mean particle size ranging from about 0.01 microns to about 15 microns, preferably from about 0.05 microns to about 10 microns, more preferably from about 0.1 microns to about 5 microns and most preferably from about 0.2 microns to about 0.3 microns. The silica preferably has a surface area greater than 50 sq. m/gram, more preferably greater than about 130 sq. m./gram, most preferably greater than about 180 sq. m./gram. When amoφhous silicas are used as the stabilizer herein, they are typically included in the cleansing compositions at levels ranging from about 0.1% to about 10%, preferably from about 0.25% to about 8%, more preferably from about 0.5% to about 5%. A fourth class of stabilizer which can be employed in the antimicrobial cleansing compositions of the present invention comprises dispersed smectite clay selected from the group consisting of bentonite and hectonte and mixtures thereof. Bentonite is a colloidal aluminum clay sulfate. See Merck Index, Eleventh Edition, 1989, entry 1062, p. 164, which is incoφorated by reference. Hectonte is a clay containing sodium, magnesium, lithium, silicon, oxygen, hydrogen and flounne. See Merck Index, eleventh Edition, 1989, entry 4538, p. 729, which is herein incoφorated by reference. When smectite clay is employed as the stabilizer in the cleansing compositions of the present invention, it is typically included amounts ranging from about 0.1% to about 10%, preferably from about 0.25% to about 8%, more preferably from about 0.5% to about 5%. Other known stabilizers, such as fatty acids and fatty alcohols, can also be employed in the compositions herein. Palmitic acid and launc acid are especially preferred for use herein. Other Optional Ingredients The compositions of the present invention can comprise a wide range of optional ingredients. The CTFA International Cosmetic Ingredient Dictionary. Sixth Edition, 1995, which is incoφorated by reference herein in its entirety, descnbes a wide vanety of nonlimitmg cosmetic and pharmaceutical ingredients commonly used m the skm care industry, which are suitable for use the compositions of the present invention. Nonlimitmg examples of functional classes of ingredients are descnbed at page 537 of this reference. Examples of these functional classes include: abrasives, anti-acne agents, anticakmg agents, antioxidants, binders, biological additives, bulking agents, chelatmg agents, chemical additives, colorants, cosmetic astnngents, cosmetic biocides, denaturants, drug astringents, emulsifiers, external analgesics, film formers, fragrance components, humectants, opacifying agents, plasticizers, preservatives, propellants, reducing agents, skm bleaching agents, skm-conditioning agents (emollient, humectants, miscellaneous, and occlusive), skm protectants, solvents, foam boosters, hydrotropes, solubilizmg agents, suspending agents (nonsurfactant), sunscreen agents, ultraviolet light absorbers, and viscosity increasing agents (aqueous and nonaqueous) Examples of other 00/61105 24 functional classes of materials useful herein that are well known to one of ordinary skill m the art include solubilizmg agents, sequestrants, and keratolytics, and the like. II. CHARACTERISTICS The antimicrobial wipes herein, have the following charactenstics. Gram Negative Residual Effectiveness Index The antimicrobial cleansing compositions of the present invention have a Gram Negative Residual Effectiveness Index of greater than about 0.3 (50% reduction), preferably greater than about 1.0 (90% reduction), and more preferably greater than about 2.0 (99% reduction) The Gram Negative Residual Effectiveness Index is measured by the In- Vivo Residual Effectiveness on Escherichia coli Test described hereinafter m the Analytical Methods Section. The index represents a difference m base ten logarithm values of bacterial concentrations between a test sample and a control. For example, an index of 0.3 represents a reduction in log values of 0.3 (Δ log = 0.3) which m turn represents a 50% reduction of bacteria counts. Mildness Index The antimicrobial cleansing compositions of the present invention can optionally have a Mildness Index of greater than about 0.3, preferably greater than about 0.4, and more preferably greater than about 0.6. The Mildness Index is measured by the Forearm Controlled Application Test (FCAT) described herein. Ill PREPARATION OF THE ABSORBENT SHEETS IMPREGNATED WITH ANTIMICROBIAL CLEANSING COMPOSITION Any method suitable for the application of aqueous or aqueous/alcoholic impregnates, including flood coating, spray coating or metered dosing, can be used to impregnate the fibrous webs herein with the antimicrobial cleansing compositions described herein. More specialized techniques, such as Meyer Rod, floating knife or doctor blade, which are typically used to impregnate liquids into absorbent sheets may also be used. The emulsion should preferably comprise from about 100% to about 400%, preferably from about 200% to about 400% by weight of the absorbent sheet After coating, the sheets may be folded into stacks and packaged in any of the moisture and vapor impermeable packages known in the art The anti-microbial cleansing compositions of the present invention are made via art recognized techniques for the various forms compositions IV. METHODS OF USING THE ANTIMICROBIAL WIPES The antimicrobial wipe of the present invention are useful for personal cleansing and providing residual effectiveness versus Gram negative bacteria, especially on the hands and face. Typically the wipe is used to apply cleansing compositions to the area to be cleansed. The wipes herein can be used for personal cleansing when the use of cleansing products requmng water cannot be, or are inconvenient. Typical quantities of the present wipes useful for cleansing, range from about about 1 to about 4 wipes per use, preferably from about 1 to about 2 wipes per use. Typical amounts of antimicrobial cleansing composition used range from about 4 mg/cm^ to about 6 mg/cm^, preferably about 5 mg/cm^ of skm area to be cleansed. ANALYTICAL TEST METHODS MICROTOX RESPONSE TEST Reference: Microtox Manual: A Toxicitv Testing Handbook. 1992 Volume I-IV, Microbics Coφoration. Equipment: Microtox M500 Toxicity Testing Unit; Microbics Coφoration Connected to computer for data acquisition and analysis according to above reference. Procedure: 1. Preparation of Sample Stock Solution (Standard Concentration: 1000 ppm The stock solution of the test anionic surfactant sample is prepared and used as a stock solution from which all other dilutions are made. The standard "starting concentration", the highest concentration to be tested, is 500 ppm. (If a 500 ppm starting concentration fails to give a calculable result, e.g. an active surfactant kills all reagent at all dilutions, the starting concentration can be adjusted based on a known range of EC50 values of previously tested surfactants.) The stock solution is prepared at two times the starting concentration. a) Add O.lg (or adjusted amount if required) of amonic surfactant, accounting for activity of raw matenal, to beaker. b) Microtox Diluent (2% NaCl, Microbics Coφ.) is added to total lOOg. c) Stir solution to make sure of adequate mixing. 2 Reconstitution of Microtox Reagent and Preparation of Assay a) Turn on test unit and allow reagent well temperature to equilibrate at 5.5°C and incubator block and read well temperature to equilibrate at 15°C. b) Place a clean cuvette (Microbics Coφ.) in the reagent well, and fill \\ ith 1.0 ml of Microtox Reconstitution Solution (distilled water, Microbics, Coφ.). Allow to cool for 15 minutes c) Reconstitute standard vial of Microtox Acute Toxicity Reagent ( Vibrio fischerio, Microbics Coφ.) by quickly adding the 1.0 ml of the cooled reconstitution solution to the reagent vial. d) Swirl solution the reagent vial for 2-3 seconds then pour reconstituted reagent back into the cooled cuvette and return the vial to the reagent well. Allow to stabilize for 15 minutes. e) Place 8 cuvettes containing 500 μl of Microtox Diluent, as assay, into the incubator wells of the test unit. Let cool for 15 minutes. 3. Test Substance Dilution Prepare 7 senal dilutions of the test substance from the sample stock solution. The final volume of all cuvettes must be 1.0 ml. a) Place 8 empty cuvettes into a test tube rack. b) Add 1.0 ml of Microtox Diluent solution to tubes 1-7. c) Add 2.0 ml of the sample stock solution (1000 ppm) in cuvette 8. d) Transfer 1.0 ml solution from cuvette 8 to cuvette 7 and mix cuvette 7. e) Senally transfer 1.0 ml from the newly formed solution to the subsequent cuvette (7 to 6, 6 to 5 etc.). Remove 1.0 ml of solution from cuvette 2 and discard. Cuvette 1 is the blank containing only Microtox Diluent. Place the cuvettes into the test unit incubation wells keeping them in order of lowest to highest concentration. These cuvettes should correspond with the 8 cuvettes prepared m step 2 above. Allow to cool for 15 minutes. Assay and Sample Bioluminescence Testing a) Add 10 μl of reconstituted reagent to the 8 precooled cuvettes of assay prepared in step 2 above (containing 500 μl of diluent). Allow 15 minutes for reagent to stabilize. b) Start Microtox Data Capture and Reporting Software (Microbics Coφ.), select START TESTING, input file name and descnption, correct starting concentration m ppm (500 if standard concentration is used) and number of controls (1) and dilutions (7). Time 1 should be selected as 5 minutes, time 2 is NONE. Press enter then the space bar to begin testing. c) Place the assay cuvette containing reagent which corresponds to the test blank into the read well and press SET. After the cuvette has resurfaced press READ and the value will be captured by the computer. d) Similarly read the remaining 7 cuvettes containing reagent when prompted by the computer by pressing the READ button with the correct cuvette m the READ well. e) After all 8 initial reading have been taken, transfer 500 μl of the diluted test substance into their corresponding cuvette containing the reagent. Mix by vortex g or swirling and return to the incubation wells The computer will count for five minutes and prompt you to begin final readings. f) Take final readings by placing the correct cuvette containing reagent and diluted test surfactant into the read well and pressing READ when prompted by the computer. 5. Data Analysis The concentration of test substance, in ppm, that decreases the bioluminescence of the Microtox Acute Toxicity Reagent by 50% from the starting value (EC50 Value) can be calculated using the Run Statistics on Data File option of the Microtox Software (recommended) or by conducting a linear regression of the data (% reduction vs. log of concentration). % Reductions are calculated using the following formulas: Final Reading of Reagent Blank _ = Correction Factor Initial Reading of Reagent Blank Final Reading of Reagent with Diluted Test Substance -- , -, -- 2 - = Reduction Factor x Initial Reading of Reagent with Diluted Test Substance where x means at a corresponding concentration „, „ , Correction Factor v - Reduction Factor % Reduction = - Correction Factor The Microtox Index is the EC50 value m ppm. IN VIVO RESIDUAL EFFECTIVENESS ON E colt References: Aly, R; Maibach, H.I.; Aust, L.B.; Corbin, N.C.; Fmkey, M.B. 1994. 1. In vivo effect of antimicrobial soap bars containing 1 5% and 0.8% trichlorocarbanilide against two strains of pathogenic bacteria J. Soc. Cosmet Chem., 35, 351-355, 1981. 2 In vivo methods for testing topical antimicrobial agents. J. Soc. Cosmet. Chem., 32, 317-323. 1. Test Design Residual Antibacterial efficacy of liquid and bar soap antimicrobial products are quantified in the following method Reductions are reported from a control, non-antibacterial placebo soap, without further treatment, used on one of the subjects forearms. By definition the antibacterial placebo will show no residual effectiveness m the test 2 Pre-Test Phase Subjects are instructed not to use antibacterial products for 7 days prior to testing. Immediately before test, the subjects hands are examined for cuts broken skm that would preclude them from participating. 3. Wash Procedure for Wipes Test Product a) Wash both forearms with placebo soap one time to remove any contaminants or transient bactena. Rinse and dry forearms. b) Test monitor marks 10cm x 5cm treatment area on forearm. c) Test monitor wipes the treatment site with appropnate wipe in an up-and-down motion for 10 seconds. d) Arm is allowed to air dry and test sites are marked (~8.6 cm^ circle with rubber stamp). e) Mark site with stamp on other forearm of subject for placebo product evaluation. 4. Inoculation Procedure a) E. coli inoculum (ATCC 10536, grown from Iyophilized stock in Soybean-casein broth at 37C for 18-24 hrs) is adjusted to approximately 10° organisms/ml (0.45 transmittance vs. TSB blank on specrophotometer). b) Each test site is inoculated with 10 μl of E. coli. Inoculum is spread with inoculating loop into a ~3 cm^ circle and covered with a Hilltop Chamber (Hilltop Research Inc.). c) This procedure is repeated for each test site on each forearm. 5. Sampling Bacteria (Extraction Procedure) a) Prepare sampling solution of 0.04% KH2PO4, 1.01% Na 2 HP0 4 , 0.1% Triton X- 100, 1.5% Polysorbate 80, 0.3% Lecithin in water, adjusted to pH 7.8 with 1 N HC1. b) Exactly 60 minutes after inoculation, the Hilltop Chamber is removed from the site from which a sample is to be taken. A 8.6 cm^ sampling cup in placed over the site c) 5 ml of sampling solution is added to the cup. d) Extract the bacteria by gently rubbing site with glass police man for 30 seconds. e) Remove sampling solution with pipette and place a sterile labeled test tube. f) Repeat extraction with 5 ml of sampling fluid This entire extraction procedure is repeated for each site 60 minutes after inoculation. 6 Quantifying Bacteria a) Prepare phosphate buffer solution of 0.117% Na 2 HP0 , 0.022% NaH 2 P0 4 . and 0.85% NaCl adjusted to pH 7.2-7.4 with 1 N HC1. b) 1.1 ml of the sampling solution is aseptically removed from the tube, 0.1 ml of the solution is spread plated onto trypticase-soy agar containing 1.5% Polysorbate 80 Remaining 1 ml is placed into 9 ml of sterile phosphate buffer achieving a 1 : 10 dilution of the sampling solution. This process is repeated 3 more times (each serial dilution). c) The plates are inverted and incubated for 24 hours at 35C. d) Colonies formed on plates are then enumerated and results are calculated by multiplying the counts by the dilution factor (original sample = 10, first dilution = 100, second dilution = 1000, etc.) and the final results are reported as the number of colony forming units per ml (CFU's/ml). 7. Index Calculation Gram Negative Residual Efficacy Index = log ι ø (CFU's/ml of placebo site) - logjo (CFU's/ml of test product site) FOREARM CONTROLLED APPLICATION TEST f FCAT) Reference: Ertel, K. D., et al.; "A Forearm Controlled Application Technique for Estimating the Relative Mildness of Personal Cleansing Products"; J. Soc. Cosmet. Chem. 46 (1995) 67-76 The Forearm Controlled Application Test, or FCAT, is a comparative test which discnmmates differences m product mildness to the skm. A test product is compared to a standard soap based cleansing bar control. Test Group Restnctions Test groups of 20-30 subjects, 18 to 55 years of age, who regularly wash with soap are used. Potential subjects who (1) have an initial dryness grade of 3.0 or higher on the forearms as assessed during the initial examination, (2) have skin cancer, eczema, or psonasis on the forearms, (3) are receiving mjectable msulm, (4) are pregnant or lactatmg, or (5) are receiving treatment for skm problems or contact allergy are excluded. Subjects are to avoid hot tubs, swimming, and sun lamps, and to refrain from applying any soaps, cleansing products, creams, or gels to their forearms for the duration of the study. Subjects are to keep water off their forearms for at least two hours before the grading process. The studies are executed using a blinded, random product order format. Clinical assistant should verify the correct treatment sequence and document such before washing each subject. Products are applied to the forearms a total of nine (9) times: two (2) times each day on the first four (4) days of the study and one (1) time on the final day. Visits to the test facility for washing must be spaced by a minimum of three (3) hours All clinical assistants must wear disposable gloves during wash procedure, nnsmg them between treatments, and changing between subjects Control Product The control product is a rolled bar soap containing: 56.1% Sodium Tallowate 18.7% Sodium Cocoate 0.7% Sodium Chloride 24% Water 0.5% Minors (Perfume, impurities, etc.) Product Application Procedure Both test and control products are tested on the same arm. The following test procedure is used. 1 The subject wets the entire surface of his/her volar forearm with 95-100°F tap water by holding the arm bnefly under running tap water. 2. A clinical assistant wets one-quarter sheet (approximately 8" x 6") of Masshnn® towel with tap water, then squeezes the towel gently to remove excess water. 3. A clinical assistant applies the products to the arm, beginning with the product designated for the site nearest the elbow, using the appropriate procedure as follows: Liquid Product a Dispense 0.10 cc of test product from a syringe into the center of the appropnate marked area b Wet two finders of gloved (latex) hand under the running tap (index and middle fingers), c Move wetted fingers m a circular motion over the application site for 10 seconds to lather product. d Lather remains on the application site for 90 seconds, then is nnsed off with running tap water for 15 seconds, taking care not to wash lather off the adjacent sites. After 10 seconds of the nnse has expired, the Clinical Assistant will gently rub the site being rinsed with her two gloved fingers for the remaining 5 seconds of the rinse.. Bar Product a Wet two finders of gloved (latex) hand under the running tap (index and middle fingers). b Wet bar by holding bar briefly under running tap water. Test bars must be wet under a running tap at the start of each da}' c Rub wetted fingers in a circular motion, over the surface of the bar, for 15 seconds to form lather on bar and fingers d Rub the lathered fingers on the application site a circular motion for 10 seconds to lather product on the skin. e Lather remains on the application site for 90 seconds, then is rinsed off with running tap water for 15 seconds, taking care not to wash lather off the adjacent sites. After 10 seconds of the nnse has expired, the Clinical Assistant will gently rub the site being nnsed with her two gloved fingers for the remaining 5 seconds of the nnse. Wipe Products a. Fold wipe in half, crosswise, and gently rub the wipe in a curncular motion within the appropnate area. b Allow site to air dry for 90 seconds. Do not nnse site. Leave-on Product a. Dispense 0.10 cc of test product from a synnge into the center of the appropnate marked area. b. Move gloved fingers m a circular motion over the application site for 10 seconds. c. Allow site to air dry for 90 seconds. Do not nnse site. 4 While waiting for the 90 second residence time to expire, the above procedure will be repeated on the remaining apphcation site on that arm, working down the arm toward the wnst. 5 Steps 1-4 are repeated on the appropriate test areas so two applications of product are made to test areas. 6 After all of the application areas have two applications of products, the clinical assistant gently pats the subject's arm dry with a disposable paper towel. Evaluation The skm on each treatment area is evaluated by an expert grader at baseline and three hours after the final study wash The treatment areas are evaluated under 2.75x magnification (model KFM-IA Luxo Illuminated Magnifying Lamp, Marshall Industries, Dayton, OH) with controlled lighting (General Electric Cool White, 22-watt, 8" Circulme fluorescent bulb). The skin is evaluated by an expert grader ,for dryness and a rating is assigned based on the definitions set forth below. Table 1 Forearm Grading Scale Rating Skin Dryness 0 No dryness 1 0 Patches of slight powdeπness and occasional patches of small scales may be seen 2 0 Generalized slight powdermess Early cracking or occasional small lifting scales may be present 3.0 Generalized moderate powdermess and/or heavy cracking and lifting scales. O 00/61105 32 4.0 Generalized heavy powdenness and/or heavy cracking and lifting scales 5.0 Generalized high cracking and lifting scales. Eczematous change may be present. Powdenness may be present but not prominent. May see bleeding crack. 6.0 Generalized severe cracking. Eczematous change may be present. Bleeding cracks may be present. Scales large, may be beginning to disappear. The FCAT generally produces only mild to moderate skm lmtation; however, if a treated site reaches a rating of 5.0 or greater, at any time dunng the study, treatment of all sites on that subject should be discontinued. Data After all subjects have been evaluated at the end of the test, the following values are determined: Rc 0 = The average rating of control product area at baseline Rcf = The average rating of control product area at test end Rt 0 = The average rating of test product area at baseline Rtf = The average rating if test product area at test end. There are many external conditions which could influence the FCAT, such as relative humidity and water softness. The test is valid only if sufficient response is observed m the skm to the control product. The control response must be greater than 1.0 (i.e., Rcf - Rc 0 > 1.0) for the test to be valid. Given a valid test, the Mildness Index of the test product is the difference in the skm responses to two products. Mildness Index = ( Rcf - Rc 0 ) - ( Rtf - Rt 0 ) CONSISTENCY (k AND SHEAR INDEX (n) OF THE LIPOPHILIC SKIN MOISTURIZING AGENT The Cammed CSL 100 Controlled Stress Rheometer is used to determine Shear Index, n, and Consistency, k, of the hpophilic skm moisturizing agent used herein. The determination is performed at 35°C with the 4 cm 2° cone measuring system typically set with a 51 micron gap and is performed via the programmed application of a shear stress (typically from about 0.06 dynes/sq. cm to about 5.000 dynes/sq. cm) over time. If this stress results m a deformation of the sample, I e. strain of the measuring geometry of at least 10-4 rad sec, then this rate of strain is reported as a shear rate These data are used to create a viscosity μ Vs. shear rate γ' flow curve for the material This flow curve can then be modeled order to provide a mathematical expression that describes the material's behavior within specific limits of shear stress and shear rate These results were fitted with the following well accepted power law model (see for instance Chemical Engineering, by Coulson and Richardson, Pergamon, 1982 or Transport Phenomena by Bird, Stewart and Lightfoot, Wiley. 1960) O 00/61105 33 Viscosity, μ = k (γ') n~ 1 VISCOSITY OF THE ANTIMICROBIAL CLEANSING COMPOSITION The Wells-Brookfield Cone/Plate Model DV-II+ Viscometer is used to determine the viscosity of the antimicrobial cleansing compositions herein. The determination is performed at 25°C with the 2.4 cm° cone (Spindle CP-41) measuring system with a gap of 0.013 mm between the two small pms on the respective cone and plate. The measurement is performed by injecting 0.5 ml of the sample to be analyzed between the cone and plate and rotating the cone at a set speed of 1 φm. The resistance to the rotation of the cone produces a torque that is proportional to the shear stress of the liquid sample. The amount of torque is read and computed by the viscometer into absolute centipoise units (mPa's) based on geometnc constants of the cone, the rate of rotation, and the stress related torque. ABSORBENT CAPACITY Substrate samples are placed m a temperature and relative humidity-controlled location for at least 2 hours pnor to testing (temperature= 73°F ± 2°F, relative humidity 50% ± 2%). A full size substrate sheet is supported horizontally in a tared filament lined basket and weighed to provide the weight of the dry sheet. The filament lined basket has crossed filaments which serve to support the sheet honzontally. The crossed filaments permit unrestricted movement of water into and out of the substrate sheet. The substrate sheet, still supported in the basket, is lowered into a distilled water bath having a temperature of 73°F ±2°F for one mmute. The basket is then raiesed from the bath and the substrate sheet is allowed to drain for 1 mmute. The basket and sheet are then re-weighed to obtain the weight of the water absorbed by the substrate sheet The Absorbent Capacity, m grams/gram, is calculated by dividing the weight of the water absorbed by the sheet by the weight of the dry sheet, the Absorbent Capacity is reported as an average of at least 8 measurements EXAMPLES The following examples further describe and demonstrate embodiments withm the scope of the present invention. In the following examples, all ingredients are listed at an active level. The examples are given solely for the puφose of illustration and are not to be construed as limitations of the present invention, as many variations thereof are possible without departing from the spirit and scope of the invention Ingredients are identified by chemical or CTFA name Fifteen antimicrobial cleansing compositions are prepared according to the tables below Antimicrobial Cleansing Compositions Component Ex. 1 Ex. 2 Ex. 3 Ex. 4 Ex. 5 O 00/61105 35 O 00/61105 36 The antimicrobial cleansing compositions shown all have a Gram Negative Residual Effectiveness Index of greater than about 0.3; and a Mildness Index of greater than 0.3. Procedure for Making Antimicrobial Cleansing Composition Examples When mineral oil is used, premix mineral oil, propylene glycol, active, steareth 2 and 20, oleth 2 and 20, and 50%, by weight of the oil, glycol, active, steareth and oleth matenals, water to a premix vessel. Heat to 165°F ± 10°F. Add additional 50%, by weight of the oil, glycol, active, steareth and oleth matenals, of water to the premix tank. Add all but 5 weight percent of remaining water to second mix tank. If required, add premix to the mix tank. Add surfactants to mix tank. Heat matenals to 155°F ±10°F and mix until dissolved. Cool to less than 100°F, add acid and antibactenal active, if not m premix, and perfumes Mix until matenals are dissolved. Adjust pH to target with required buffer (NaOH or buffer salt). Add remaining water to complete product. Procedure for Making Antimicrobial Wipe Examples Compositions 1-15 are impregnated onto absorbent sheets as follows: Composition 1-15 are impregnated onto a wet and air laid woven absorbent sheet compnsed of 85% cellulose and 15% polyester at 260% by weight of the absorbent sheet by pounng the composition onto the sheet via a cup. Composition 1-15 are impregnated onto a wet and air laid woven absorbent sheet comprised of 100% cellulose at 260% by weight of the sheet by pounng the composition onto the sheet via a cup. Compositions 1-15 are impregnated onto separate wet and air laid nonwoven absorbent sheets comprised of 50% cellulose and 50% polyester at 260% by weight of the sheet by pounng the compositions onto the sheets via a cup.

Claims: WHAT IS CLAIMED IS: 1 An antimicrobial wipe comprising a porous or absorbent sheet impregnated with an antimicrobial cleansing composition, wherein the antimicrobial cleansing composition compnses: a. from about 0.001% to about 5.0%, by weight of the antimicrobial cleansing composition, of an antimicrobial active; b. from about 0.05% to about 10%, by weight of the antimicrobial cleansing composition, of an anionic surfactant; c from about 0.1% to about 10%, by weight of the antimicrobial cleansing composition, of a proton donating agent; and d. from about 3% to about 99.85%, by weight of the antimicrobial cleansing composition, water; wherein the composition is adjusted to a pH of from about 3.0 to about 6.0; wherein the antimicrobial cleansing composition has a Gram Negative Residual Effectiveness Index of greater than about 0.3. 2. An antimicrobial wipe compnsmg according to Claim 1 which also has a Mildness Index of greater than about 0.3. 3. An antimicrobial wipe according to Claim 2 wherein the antimicrobial active is selected from the group consisting of Tnclosan®, Tnclocarban®, Octopirox®, PCMX, ZPT, natural essential oils and their key ingredients, and mixtures thereof 4. An antimicrobial wipe according to Claim 3 wherein the antimicrobial active is Tnclosan®. 5. An antimicrobial wipe according to Claim 3 wherein the anionic surfactant has a Microtox Index of less than about 150 6 An antimicrobial wipe according to Claim 3 wherein the proton donating agent is an organic acid having a Buffenng Capacity of greater than about 0.005 7. An antimicrobial wipe according to Claim 5 wherein the proton donating agent is an organic acid having a Buffering Capacity of greater than about 0.005. O 00/61105 38 8 An antimicrobial wipe according to Claim 3 wherein the proton donating agent is a mineral acid. 9. An antimicrobial wipe according to Claim 3 wherein the composition is adjusted to a pH of from about 3.5 to about 5.0. 10. An antimicrobial wipe according to Claim 7 wherein the composition is adjusted to a pH of from about 3.5 to about 5.0. 11. An antimicrobial wipe according to Claim 9 wherein the ratio of the amount of non-anionic surfactants to the amount of anionic surfactant compnsmg the antibacterial cleansing composition is less than 1 :1. 12. An antimicrobial wipe according to Claim 10 wherein the ratio of the amount of non- amonic surfactants to the amount of amonic surfactant comprising the antibactenal cleansing composition is less than 1:1. 13. An antimicrobial wipe comprising a porous or absorbent sheet impregnated with an antimicrobial cleansing composition, wherein the antimicrobial cleansing composition comprises: a. from about 0.001% to about 5.0%, by weight of the antimicrobial cleansing composition, of an antimicrobial active; b. from about 0.05% to about 10%, by weight of the antimicrobial cleansing composition, of an anionic surfactant; c from about 0.1% to about 10%, by weight of the antimicrobial cleansing composition, of a proton donating agent; d. from about 0.1% to about 30% by weight of the antimicrobial cleansing composition of a hpophilic skm moisturizing agent; and e. from about 3% to about 99.85%, by weight of the antimicrobial cleansing composition, water; wherein the composition is adjusted to a pH of from about 3.0 to about 6.0; wherein the antimicrobial cleansing composition has a Gram Negative Residual Effectiveness Index of greater than about 1.0; and wherein the antimicrobial cleansing composition has a Mildness Index of greater than about 0.4. 14. An antimicrobial wipe according to Claim 13 comprising from about 0.1% to about 2%, by weight of the antimicrobial cleansing composition, of the anionic surfactant. 15 An antimicrobial wipe according to Claim 14 wherein the proton donating agent is an organic acid and has a buffenng capacity of greater than 0.01. 16. An antimicrobial wipe according to Claim 15 wherein the amonic surfactant is selected from the group consisting of sodium and ammonium alkyl sulfates and ether sulfates having chain lengths of predominantly 12 and 14 carbon atoms, olefin sulfates having chain lengths of predominantly 14 and 16 carbon atoms, and paraffin sulfonates having an average chain length of from 13 to 17 carbon atoms, and mixtures thereof. 17 An antimicrobial wipe according to Claim 18 wherein the composition is adjusted to a pH of from about 3.5 to about 5.0. 18 An antimicrobial wipe according to Claim 17 wherein the proton donating agent is selected from the group comprising adipic acid, tartanc acid, citric acid, maleic acid, malic acid, succmic acid, glycohc acid, glutaπc acid, benzoic acid, malonic acid, salicylic acid, glucomc acid, polyacryhc acid, their salts, and mixtures thereof. 19. A method for providing improved residual effectiveness against Gram negative bactena compnsmg the use of a safe and effective amount of the composition of Claim 1 on human skm. 20 A method for providing residual effectiveness against Gram negative bactena which compnses the use of a safe and effective amount of the composition of Claim 13 on human skm. 21. A method for treating acne comprising the use of a safe and effective amount of the composition of Claim 1 on human skm.

Inventors: BAIER KATHLEEN GRIESHOP, BAKKEN THERESA ANNE, BEERSE PETER WILLIAM, CEN RAYMOND WEI, MORGAN JEFFREY MICHAEL, BAIER, KATHLEEN, GRIESHOP, BAKKEN, THERESA, ANNE, BEERSE, PETER, WILLIAM, CEN, RAYMOND, WEI, MORGAN, JEFFREY, MICHAEL
Applicants: PROCTER & GAMBLE, THE PROCTER & GAMBLE COMPANY
Application year: 2000
Main classification: A61K7
IPC codes: A61K8, A61Q19, C11D1, C11D3, C11D17