USE OF D-TAGATOSE AS SYNERGISER AND FLAVOUR ENHANCER

Abstract: D-tagatose is even in small amounts a powerful synergiser for potent sweeteners. Further, it is able to effect improvements in the quality of flavour and taste of the products in which it is used.

Description: Use of D-tagatose as synergiser and flavour enhancer. This invention relates to use of D-tagatose to synergise the effect of potent sweeteners used for sweetening of food formulations. D-tagatose is even in very small amounts a powerful synergiser for potent sweeteners. Further, it is able to effect improvements in the quality of taste of the food products in which it is used. D-tagatose is a well-known keto-hexose which is useful as a reduced calorie food sweetening and bulking agent, and as an additive in detergent, cosmetic and pharmaceutical formulations. U.S. Patent Nos. 5,002,612 and 5,078,796 to Beeadle et al . teach processes of making D-tagatose by iso erizing a mixture containing D-galactose with a metal hydroxide in the presence of a catalyst at a relatively low temperature to form an intermediate complex, followed by neutralising with acid to yield D-tagatose. D-tagatose is known as an anti-hyperglycaemic agent that can be used to inhibit formation of advanced glycosyla- tion in products in mammals as described in U.S. Patents 5,356,879 and 5,447,917 to Zehner et al . D-tagatose also is known as a low-calorie carbohydrate sweetener, and bulking agent that can be used in the preparation of sweetened edible formulations in stead of sucrose as taught in U.S. Patent 4,786,722 to Zehner. While the invention has been described with respect to the use of D-tagatose as the sole sweetening agent, also a combina- tion with conventionally used sweetening agents has been proposed, e.g. in combination with a minor amount of sucrose. Furthermore, it has been proposed to use D- tagatose as a bulking and sweetening agent in combination with one of the intensive sweeteners such as aspartame or saccharin. If D-tagatose should be used as sole sweetener in products its use levels will never be below 5%, proba- bly rarely under 8% and often 10% or more. As a bulking agent for low calorie sweeteners the use level will be over 15% for bakery products and frozen dairy products, more than 20% for chocolate and 50% for candies. US pat- ent 4,786,722 does not teach the use of D-tagatose for the sensory impact it has on other sweeteners. The use of low calorie sweeteners, such as potent sweeteners, is common in a variety of food products and bever- age formulations. For many consumers, however, it is a problem that they do not like diet or light products sweetened with potent sweeteners because of their mouth ¬ feel and aftertaste. Attempts have been made over the years to improve the taste delivery of these diet or light products through the addition of small quantities of carbohydrates. Diet beverages may, on occasion, contain small quantities of fructose added to improve the taste delivery. These products generally contain approximately 1 percent fruc ¬ tose. Other products, particularly in Japan, may contain erythritol as an additive to improve other qualities of the food or beverage product beyond its sweetness. For example, other sensory characteristics of a food or bev- erage product include the flavour, mouthfeel and aftertaste of the product. Efforts have been made to add spe ¬ cific small molecular weight carbohydrates to diet or light beverages in an attempt to improve the sensory delivery. Efforts continue to identify a low-calorie sweetener to deliver sucrose-identical sensory characteristics in edible food products, and particularly with respect to beverages. Low-calorie potent sweeteners generally permit the development of diet or light products through an ability to elicit sufficient sweetness at a very low con- centration of the sweetener, but they are generally unable to mimic other sensory characteristics of sucrose, particularly mouthfeel/body . In addition, artificial sweeteners to date have not been able to deliver the full, rounded, sweetness typical of sucrose and other small molecular weight carbohydrates. Accordingly, there is a continuing need to identify a low-calorie sweetener which provides both the sweetness characteristics of sucrose and other important sensory characteristics such as mouthfeel, flavour, and aftertaste. It has now been found that D-tagatose is not only able to effect flavour improvements in the quality of food and beverage formulations, particularly in diet/light bever- ages, but that its use is highly synergistic with potent sweeteners. The synergistic effect of D-tagatose with low calorie potent sweeteners is present even at very low addition rates. The use of D-tagatose is able to replace significant quantities of potent sweeteners, even when it is added at concentrations well below its measured sweet taste threshold. Not only does the use of D-tagatose have benefits in diet or light beverages from a synergy standpoint, other im- portant qualitative benefits from the use of D-tagatose can be realised in these types of products. Subtle, but consistent, benefits have been observed through the use of D-tagatose with respect to flavour, aftertaste, and mouthfeel of beverages containing as little as 0.2% D- tagatose. Thus the invention relates to the use of D-tagatose both as a synergiser and as a flavour enhancer for potent sweeteners . The benefits of the use of D-tagatose with one or more potent sweeteners can be realised with concentrations of D-tagatose of about 0.01 to about 5% weight/volume based on the total composition. The amount of potent sweetener in the total composition will typically range from about 5 ppm to about 4000 ppm (mg/kg of total composition) depending on the sweetener used. The weight ratio of D- tagatose to potent sweetener in the sweetener composition according to the present invention ranges from 1:5 to 10,000:1. The particular ratio selected is dependent on the potent sweetener (s) used and the desired level of sweetness and other sensory characteristics. The use of D-tagatose also has been shown to provide benefits as a component in table-top sweetener formulations containing a potent sweetener. Synergistic combinations of sweeteners including D- tagatose can be obtained by using about 0.01 to about 5.0% (w/v) , preferably from about 0.05 to 2 % (w/v) , and most preferably from about 0.1 to about 1% (w/v) of D- tagatose. The amount will depend on the desired level of both sweetness and other sensory characteristics includ ¬ ing mouthfeel, aftertaste, and flavour. The beneficial effects of this invention can be realised when D-tagatose is used in conjunction with one or more potent sweeteners. These potent sweeteners are well known in the art and are sometimes referred to as high intensity sweeteners. Potent sweeteners include cycla- mate, saccharin, aspartame, acesulfame-K, neohesperidin dihydrochalcone, glycyrrhizin, rebaudioside, mogroside, monellin, thaumatin, alitame, neotame, stevioside and sucralose. The potent sweeteners can be used as sole potent sweetener or as a mixture of more potent sweeten- ers, usually two or three. The desired weight ratio of D-tagatose to potent sweetener (s) will depend on the sweetener or sweetener combination, and the sweetness and other characteristics desired in the final product. Potent sweeteners vary greatly in their potency, ranging from about 30x sucrose for cyclamate to about 8000x sucrose for neotame . In general, the weight ratio of D-tagatose to potent sweetener in the sweetener composition according to the present invention will range between 1:5 and 10,000 : 1 , typically between 1:1 and 1000:1, and preferably between 4:1 and 200:1, depending on the particular potent sweetener (s) selected. Thus, if D-tagatose is blended with cyclamate, and D-tagatose was added to a beverage in an amount of 0.2% (w/v), approximately the same amount of cyclamate would be required to deliver an acceptable amount of sweetness. In this case, a ratio of 1:1 would be appropriate. If D-tagatose was combined with neotame in an amount of 1% (w/v) of the total composition, the concentration of neotame required for similar sweetness levels would be about 0.001%, or a ratio of 1000:1. Other ratios that are suitable for other potent sweeteners within the scope of the present invention will be apparent to those skilled in the art. Taking acesulfame- K, for example, at about 150x sucrose, 0.05% D-tagatose may be combined with 0.05% acesulfame-K (1:1 ratio), or 5.0% D-tagatose may be combined with 0.01% acesulfame-K (500:1 ratio). For aspartame at about 200x sucrose, 0.05% D-tagatose may be combined with 0.04% aspartame (ratio of 1.25:1), or 5% D-tagatose with 0.01% aspartame (500:1 ratio). For sucralose at about 600x sucrose, 0.05% D-tagatose could be used with 0.02% sucralose (2.5:1 ratio) or 5% D-tagatose could be used with 0.005% sucralose (1000:1 ratio). For alitame at about 2000x sucrose, 0.05% D-tagatose would be combined with 0.005% alitame (10:1 ratio) or 5% D-tagatose would be combined with 0.0025% alitame ((2000:1) ratio. As is evident from these exemplary ratios, reducing the amount of D-tagatose from 5% generally will require the amount of potent sweetener to be increased. Similarly, the amount of the components of the sweetener composition in an edible food product will vary depending on the potent sweetener used and the sweetness and other characteristics desired in the final product. In a typi- cal beverage formulation, for example, at 5% D-tagatose, a concentration of about 5 ppm of potent sweeteners such as thaumatin, alitame or neotame would be suitable. If the potent sweetener were cyclamate, at 5% D-tagatose, the cyclamate would suitably be present at 500 ppm. With D-tagatose present at 0.1%, the concentrations of these potent sweeteners would be present at a concentration of about 10 ppm and 1,000 ppm respectively. Determination of appropriate concentrations to achieve results consistent with the teachings of this invention is within the skill of the art. When D-tagatose is used in conjunction with one or more potent sweeteners, it has been observed that it is able to replace significant quantities of those other sweeten- ers, even when it is added at concentrations well below its measured sweet taste threshold. A surprising amount of potent sweetener saving is made possible by the use of D-tagatose, much more than would be expected given its sweetness intensity at the level of use. A sweetener composition according to the present invention can be used with a great variety edible products including fruit, dairy products, a variety of beverages including juices and carbonated beverages, bakery prod- ucts, confections, and the like. For example, it can be added to a beverage formulation to obtain the desired balance of sweetness and other sensory characteristics of the beverage. The use of D-tagatose in combination with a potent sweetener is particularly effective in improving the mouthfeel of a beverage. In using the sweetening composition according to the present invention, it may be incorporated in the edible food product to be sweetened in any appropriate manner. For example, it may be added directly to the product to be sweetened or it may be first combined with a diluent and then added to the prod- uct or any component of the ultimate product at any stage in the manufacturing process. In order to demonstrate the synergistic effects and other benefits derived from the use of D-tagatose in combina- tion with one or more potent sweeteners, several experiments were performed. Experiment 1 Sweetness Equivalence Of D-tagatose Relative To Aspartame The sweetness of D-tagatose relative to aspartame was determined according to the following procedure. A group of six panellists were presented with a range of aspartame standard and a randomly coded solution containing D-tagatose. The panellists were asked to taste the randomly coded solution and estimate that sweetness rela ¬ tive to the standards. Each concentration of D-tagatose was tested in duplicate. The following concentrations of D-tagatose were evaluated compared to aspartame: Experiment 2 As will be described below, the quantitative sweetness synergy that can be achieved by mixing D-tagatose with aspartame and a combination of aspartame and acesulfame-K mixtures was first estimated. These mixtures were then used in conjunction with typical beverage flavour systems (cola and lemon/lime) where, in addition to quantifying the sweetness synergy, determinations were made of the impact on product mouthfeel, flavour and aftertaste. Materials used in this experiment, the procedures used, and the results obtained are reported below: (i) Model Beverage System The model system in which this Phase of the experiment was conducted is a simple citrate buffer formulated at pH 3.2. The formulation was as follows: Citric acid (anhydrous; 0.14g Trisodium citrate 0.04g Water to 100 ml This model system was selected because of its relevance to beverage formulations, while not being subject to the sensory complications induced by added flavours. (ii) Formulated Beverage Compositions (a) Lemon and Lime Formulation Ingredients % (RTD = ready to drink) Citric acid 0.310 Lemon/lime flavour 0.085 Sodium citrate 0.031 Sodium benzoate 0.018 Sweeteners see below Water to 100% Reference Sweetener Blends Aspartame 550ppm Aspartame 200ppm Acesulfame-K 200ppm (b) Cola Formulation Ingredients % (RTD) Cola flavour: 0.340 Phosphoric acid (85%) 0.100 Sodium citrate 0.031 Sodium benzoate 0.018 Citric acid 0.018 Sweeteners see below Water to 100% Reference Sweetener Blends Aspartame 525ppm Aspartame 600ppw Acesulfame-K 50ppm (iii) Sensory Procedures The sensory procedures adopted in this experiment utilised panellists fully experienced in standard sensory evaluation procedures who had been screened for their sensory acuity and ability to rank different sweetener concentrations according to their relative sweetness intensities . Equi-sweetness concentrations of all solutions were established using an established sweetness matching procedure. This procedure involved the sweetness intensity comparison of, for example, a reference 11% sucrose model soft drink buffer against that of a series of sweetener test solutions whose sweetener concentrations differed in a step-wise manner and the subsequent identification of that test product whose sweetness intensity matched pre ¬ cisely the sweetness of the reference solution. Evaluations were carried out in duplicate, with appropriate standard controls employed to eliminate such effects as adaptation, carry-over and order effects. Qualitative effects of adding D-tagatose to near-market beverages were described by reference to the equi-sweet control beverages. Panellists were instructed to comment on any differences between reference and test products in sweetness impact, time-intensity profile, mouthfeel, after-tastes including bitterness, degree of lingering sweetness and overall taste quality and acceptability. There are no generally accepted sensory methods designed to qualify "mouthfeel". However, the following approach was adopted so as to derive an estimate of the impact of D-tagatose addition on the mouthfeel of 'diet' beverages. Reference cola and lemon/lime beverages sweetened with sucrose (11 %; w/v) and sodium saccharin (0.040%; w/v) were accorded mouthfeel intensity scores of 10 and 1, respectively. Panellists were then instructed to score the mouthfeel of test beverages relative to these reference beverages. Mouthfeel values recorded represent the average scores of all panellists. (iv) Sweetener Concentrations The concentrations of the following sweeteners equi-sweet to 11% sucrose in the model beverage system were established: aspartame; aspartame : acesulfame-K (1:1); aspartame : acesulfame-K (2 : 1) ; aspartame : acesulfame-K (12:1) ; Sucrose at 11% was used as reference standard due to it being a general average sweetness equivalence for North American beverages. D-Tagatose was added at 0.2%, 0.5%, 1.0% and 1.5%, while simultaneously reducing the concentration of the low- calorie sweeteners so that equi-sweetness was maintained. These concentrations were selected so as to span the likely maximum use level. These findings were then applied to fully formulated beverages prepared to be close appropriations of commercial beverage products. Cola and lemon/ lime carbonated bever ¬ age formulations were developed and sweetened with market relevant sweetening systems, as follows: Diet/Light Cola Aspartame Aspartame : acesulfame-K Diet/Light Lemon/Lime Aspartame Aspartame : acesulfame-K The concentrations of D-tagatose identified from the model system study as inducing beneficial sweetness synergy and sweet taste quality effects were added and the level of potent sweetener adjusted to ensure equi- sweetness. The overall taste quality of the reference products and those which contained D-tagatose were compared. The sensory impact of D-tagatose addition to the model system and formulated beverages is presented in the following Tables . In Table 1, equi-sweet sweetener concentrations are presented. As the concentration of D-tagatose is increased, the amount of low-calorie sweetener needed to maintain equi-sweetness is reduced. The reduction is larger than expected from Experiment 1. It is noted, for example, that 0.2% D-tagatose is able to replace 50 ppm aspartame, where 17 ppm was expected, and 1% D-tagatose spares 170 ppm aspartame, where 43 was expected. Thus, it is clear that there is substantial synergy between D-tagatose and aspartame . The performance of D-tagatose in fully formulated cola and lemon/lime beverages is presented in Table 2. Again, equi-sweet concentrations were determined following addition of 1.0% and 0.2% D-tagatose. The sensory effects following D-tagatose addition to for- mulated beverages are described in Tables 3-6. Panel- lists evaluated the effects of two levels of D-tagatose on "flavour", "aftertaste" and "mouthfeel" attributes. TABLE 1 Model Soft Drink System: Blends Equi-sweet To 11% Sucrose T7ABLE 2 Sweetness Synergy of Blends In Soft Drink Formulations A. Lemon/Lime B. Cola TABLE 3 Sensory Evaluation of Aspartame Sweetened, Lemon/Lime Beverage TABLE 4. Sensory Evaluation of Aspartame :A_cesulfame-K Sweetened Lemon/Line Beverage T.ABLE 5 Sensory Evaluation of Aspartame Sweetened Cola Beverage 03 TABLE 6 Sensory Evaluation of Aspartame : cesulfame-K (12:1) Sweetened Cola Beverage ^o On the basis of this experiment, it can be concluded that D-tagatose induces some remarkable sensory effects when blended with potent sweeteners. D-tagatose appears to enhance sweetness in a unique manner. Addition of levels as low as 1%, at which concentration D-tagatose is barely sweet, effect a 20-25% reduction in the concentration of aspartame needed to maintain unchanged the perceived sweetness levels in beverage formulations. Even the addition of 0.2% D-tagatose has been observed to permit an almost 10% reduction in the concentration level of aspartame. This co-operation appears to be unique since such low levels of additional sweetness induce such a significant effect on perceived sweetness levels. In addition to sweetness, other benefits are derived from the use of D-tagatose. For example, at 0.2% addition to beverages, it generally exhibits a positive effect on product mouthfeel. Panellists consistently referred to the test product as having a mouthfeel perceived to be fuller than the equivalent reference beverage. Since absence of mouthfeel is generally considered to be a consequence of the use of potent sweeteners as substitutes for sucrose/high fructose syrups, it provides one way of addressing what has been considered a strong negative to consumers of diet or light beverages. It has been found that D-tagatose, when added to diet' beverages at concentrations as low as 0.2% where this increase in soluble solids, by itself, is not sufficient to cause any perceived increase in mouthfeel, enhances the perceived mouthfeel and body of such diet beverages. This enhanced mouthfeel and body is thought to be due, at least in part, to the ability of D-tagatose to convert the overall taste profile of diet beverages closer to those of regular, full-calorie beverages in keeping with a sweetness profile closer to that of sucrose. Accord- ingly, the mouthfeel and body of a D-tagatose containing beverage is also closer to that of a sucrose sweetened beverage . Additional experiments have been performed to further evaluate the sensory consequences of incorporating D- tagatose into beverages and table-top sweeteners. While the above embodiments of the present invention are illustrative, it can be readily appreciated that it would be possible to deviate from those embodiments of the present invention without departing from the spirit of the invention. As will be readily understood by those skilled in the art, the invention is capable of many modifications and improvements within the scope and spirit thereof. Accordingly, it will be understood that the invention is not to be limited by the specific embodiments but only by the scope of the appended claims.

Claims: PATENT CLAIMS 1. Use of D-tagatose to synergise the effect of a potent sweetener or a mixture of potent sweeteners. 2. Use of D-tagatose as a flavour enhancer for a potent sweetener or a mixture of potent sweeteners. 3. Use according to claim 1 or 2, c h a r a c t e r i z ' e d in that D-tagatose is used in an amount so that the weight ratio between D-tagatose and potent sweetener is about 1:5 to about 10,000:1. 4. Use according to claim 3, c h a r a c t e r i z e d in that weight ratio between D- tagatose and potent sweetener is 1:1 to 1000:1. 5. Use according to claim 1 or 2, c h a r a c t e r i z e d in that the potent sweetener is selected from the group consisting of aspartame, acesul- fame-K, saccharin, cyclamate, thaumatin, alitame, neotame, stevioside, sucralose, neohesperidin dihydrochal- cone, glycyrrhizin, rebaudioside, mogroside and monellin and a mixtures thereof. 6. Use according to claim 1 or 2, c h a r a c t e r i z e d in that the potent sweetener is a binary or tertiary mixture. 7. Use according to claim 5, c h a r a c t e r i z e d in that the potent sweetener is aspartame . 8. Use according to claim 5, c h a r a c t e r i z e d in that the potent sweetener is acesulfame-K. 9. Use according to claim 5, c h a r a c t e r i z e d in that the potent sweetener is alitame . 10. Use according to claim 5, c h a r a c t e r i z e d in that the potent sweetener is cyclamate. 11. Use according to claim 5, c h a r a c t e r i z e d in that the potent sweetener is neotame . 12. Use according to claim 5, c h a r a c t e r i z e d in that the potent sweetener is saccharin. 13. Use according to claim 5, c h a r a c t e r i z e d in that the potent sweetener is sucralose. 14. Use according to claim 6, c h a r a c t e r i z e d in that the potent sweetener is a mixture of aspartame and acesulfame-K. 15. Use according to claim 6, c h a r a c t e r i z e d in that the potent sweetener is a mixture of aspartame, acesulfame-K and cyclamate. 16. Use according to claim 7, c h a r a c t e r i z e d in that the ratio between D- tagatose and aspartame is about 1:1 to about 500:1, preferably about 4:1 to about 200:1. 17. Use according to claim 8, c h a r a c t e r i z e d in that the ratio between D- tagatose and acesulfame-K is about 1:1 to about 500:1, preferably about 4:1 to about 200:1. 18. Use according to any of the preceding claims in a liquid in such an amount that the concentration of D- tagatose in the liquid is 0.01 to 5.0 percent by weight/volume . 19. Use according to claim 18 in such an amount that the concentration of D-tagatose in the liquid is 0.05 to 2.0 percent, preferably 0. 1 to 1.0 percent by weight/volume. 20. Use according to claim 18 or 19, c h a r a c t e r i z e d in that the liquid is a beverage . 21. Use according to claim 20, c h a r a c t e r i z e d in that the beverage is a diet cola. 22. A sweetener composition comprising D-tagatose and at least one potent sweetener. 23. A composition according to claim 22, c h a r a c t e r i z e d in that weight ratio between D- tagatose and potent sweetener is about 1:5 to about 10.000:1. 24. A composition according to claim 23, c h a r a c t e r i z e d in that weight ratio between D- tagatose and potent sweetener is about 1:1 to about 1000:1. 25. A composition according to claim 22, c h a r a c t e r i z e d in that the potent sweetener is selected from the group consisting of aspartame, acesul- fame-K, saccharin, cyclamate, thaumatin, alitame, neotame, stevioside, sucralose, neohesperidin dihydrochal- cone, glycyrrhizin, rebaudioside, mogroside and monellin and a mixtures thereof. 26. A composition according to claim 22, c h a r a c t e r i z e d in that the potent sweetener is a binary or a tertiary mixture. 27. A composition according to claim 22, c h a r a c t e r i z e d in that the potent sweetener is aspartame . 28. A composition according to claim 22, c h a r a c t e r i z e d in that the potent sweetener is acesulfame-K. 29. A composition according to claim 22, c h a r a c t e r i z e d in that the potent sweetener is alitame . 30. A composition according to claim 22, c h a r a c t e r i z e d in that the potent sweetener is cyclamate . 31. A composition according to claim 22, c h a r a c t e r i z e d in that the potent sweetener is neotame. 32. A composition according to claim 22, c h a r a c t e r i z e d in that the potent sweetener is saccharin. 33. A composition according to claim 22, c h a r a c t e r i z e d in that the potent sweetener is sucralose . 34. A composition according to claim 26, c h a r a c t e r i z e d in that the potent sweetener is a mixture of aspartame and acesulfame-K. 35. A composition according to claim 26, c h a r a c t e r i z e d in that the potent sweetener is a mixture of aspartame, acesulfame-K and cyclamate. 36. A composition according to claim 27, c h a r a c t e r i z e d in that the ratio between D- tagatose and aspartame is about 1:1 to about 500:1, pref- erably about 4:1 to about 200:1. 37. A composition according to claim 28, c h a r a c t e r i z e d in that the ratio between D- tagatose and acesulfame-K is about 1:1 to about 500:1, preferably about 4 : 1 to about 200:1. 38. Use of a composition according to any of the claims 22 to 37 in a beverage in such an amount that the concentration of D-tagatose in the liquid is 0.01 to 5.0 per- cent by weight/volume. 39. Use of a composition according to any of the claims 22 to 37 in a beverage in such an amount that the concentration of D-tagatose in the beverage is 0.05 to 2.0 per- cent, preferably 0.1 to 1.0 percent by weight/volume . 40. An additive for sweetening and improving the mouthfeel of a beverage, c h a r a c t e r i z e d in that it is a composition according to any of the claims 22 to 37. 41. An additive according to claim 40, c h a r a c t e r i z e d in that the beverage is selected from the group consisting of soft drinks, coffee, tee or chocolate. 42. Soft drink, c h a r a c t e r i z e d in that it contains a composition according to any of the claims 22 to 37 in such an amount that the amount of D-tagatose is 0.01 to 5.0 per- cent, preferred o.5 to 2.0 percent and most preferred 0.1 to 1.0 percent by weight/volume.

Inventors: ANDERSEN HENRIK, VIGH MADS L, ANDERSEN, HENRIK, VIGH, MADS, L.
Applicants: ANDERSEN HENRIK, MD FOODS AMBA, VIGH MADS L, ANDERSEN, HENRIK, MD FOODS A.M.B.A., VIGH, MADS, L.
Application year: 1999
Main classification: A23L1
IPC codes: A23L1