PROTECTION FROM IONIZING IRRADIATION OR CHEMOTHERAPEUTIC DRUG DAMAGE BY IN VIVO GENE THERAPY

Abstract: A method of protecting a subject against an agent that elicits production of toxic free radicals, superoxide anions, or heavy metal cations in the subject is disclosed which entails in vivo administration to the subject of a polynucleotide encoding a protein that is transiently expressed in said subject. The transiently expressed protein is capable of neutralizing or eliminating the toxic free radicals, superoxide anions or heavy metal cations that are elicited by the agent. The method is particularly useful in preventing the development of esophagitis during treatment of lung cancer patients with ionizing radiation and/or chemotherapeutic drugs.

Description: PROTECTION FROM IONIZING IRRADIATION OR CHEMOTHERAPEUTIC DRUG DAMAGE BY IN VIVO GENE THERAPY Background of the Invention The present invention is directed generally to protecting an individual ' s tissues and cells against the damaging effects of an agent that elicits the production of a free radical, superoxide anion, or heavy metal cation when that individual is exposed to the agent. Specifically, the invention is directed to protection of the oral cavity, oropharynx, esophagus, stomach, small intestine and colon by transient expression of a protective protein through somatic gene transfer in vivo . Therapeutic concentrations of anti -cancer drugs and clinical radiation therapy are known to damage a patient's normal tissues and cells. A need clearly exists for means to protect a patient ' s normal tissues during chemotherapy and/or radiation therapy. Previous methods of affording such protection include administration of sulfhydryl compounds such as thiols or other radical scavenger compounds . The manor way m which radiation damages biomolecules and cells is through its interaction with water to produce toxic free radicals (H * , OH * , e aq ) and H 2 0 2 or, through interaction with oxygen, to produce the superoxide radicals ( * 0 2 ) . In the late 1940 's it was discovered that sulfhydryl compounds, such as cysteme and cysteamme, provide radiation protection m animals. Patt et al . , Science 110: 213 (1949). Thiol groups scavenge radiation-produced free radicals by donating a hydrogen atom to damaged molecules. Despite extensive efforts to develop more effective protective agents, no thiol -based radioprotector has been found to be significantly better than cysteamme. Mitchell et al . , Arch . Biochem . and Biophys . 289: 62 (1991). However, the use of thiol drugs to protect against radiation damage is limited by the toxicity of such compounds. Antmeoplastic agents, particularly the class of chemotherapeutic drugs known as alkylating agents, also produce free radicals that are cytotoxic due to their ability to form covalent bonds with nucleic acids. Most alkylating agents form 2 - positively charged carbonium ions that yield the charged alkylating intermediate R-CH 2 -CH 2 + which attacks electron-rich sites on nucleic acids, proteins, small molecules and ammo acids . Several endogenous mtracellular scavengers of free radicals, superoxide radicals and heavy metal cations have been identified. Induction or elevated activities of each of metallothionem (MT) , gamma-glutamyl transpeptidase (γ-GTP) and superoxide dismutase (SOD) are known to provide resistance to ionizing radiation damage in vi tro. These proteins function mtracellularly to scavenge free radicals, superoxide anions or heavy metal cations. U.S. 5,599,712, the contents of which are incorporated by reference their entirety, describes a method for providing functional mtracellular therapeutic levels of metallothionem, superoxide dismutase or gamma glutamyl transpeptidase to protect normal lung tissue from the adverse effects of a combination of chemotherapy and radiation therapy. Summary of the Invention It is therefore an object of the present invention to provide a method of protecting normal cells not at a tumor site against the damaging effects of an ant cancer agent or ionizing radiation by providing genes encoding protem protective to normal somatic cells. It is a further ob ect of the present mvention to provide a method of protecting normal cells, particularly cells of the oral cavity, oropharynx, esophagus, stomach, small intestine and colon, against the damaging effects of an anticancer agent or ionizing radiation by providing genes encoding protem protective to normal somatic cells. It is another ob ect of this mvention to provide a safe and efficient method of transferring oxidation or cation- scaveng g protem encoding genes directly into cells of the oral cavity, oropharynx, esophagus, stomach, small intestine and colon. It is yet another object of this invention to transfer oxidation or cation- scavenging protem encoding genes directly into cells of the oral cavity, oropharynx, esophagus, stomach, small intestine and colon using an easily admmistrable method. Another object of the present invention is to provide transient expression of the oxidation or cation-scavenging protein m the cells of the oral cavity, oropharynx, esophagus, stomach, small intestine and colon to protect these cells against an anticancer agent, wherein either the transferred polynucleotide or gene is cleared after therapeutic courses of ionizing radiation therapy or chemotherapy, or the transferred polynucleotide or gene is stably integrated within the genome, but its expression is temporary, and induced for a limited time by the ionizing radiation therapy or chemotherapy. In accomplishing these and other objects, there has been provided, m accordance with one aspect of the present invention, a method for protecting the oral cavity, oropharynx, esophagus, stomach, small intestine or colon in a subject against an agent that elicits production of a toxic species when the subject is exposed to the agent. Alternatively, the present invention provides a method for protecting tissues of a subject against an agent that elicits production of a toxic species when the subject is exposed to the agent, wherein the tissues to be protected are at a site remote from the tissues to be treated with toxic species. The toxic species is selected from the group consisting of a free radical, a superoxide anion, and a heavy metal cation. Each of the methods comprises administering to the subject m vivo a pharmaceutical composition comprising (A) a polynucleotide that encodes a protem that is transiently expressed in the subject, wherein the protem is capable of neutralizing or eliminating the toxic species, and (B) a pharmaceutically acceptable vehicle for the polynucleotide. The agent may be ionizing radiation, clinical radiation therapy, or a chemotherapeutic drug. In a preferred embodiment of the invention, the proteins of the invention which neutralize or eliminate the toxic species are gamma glutamyl transpeptidase, manganese superoxide dismutase, or metallothionem. In one embodiment of the invention, the pharmaceutical composition of the invention comprises a mixture of polynucleotides selected from a polynucleotide encoding gamma glutamyl transpeptidase, a - 4 - polynucleotide encoding manganese superoxide dismutase or a polynucleotide encoding metallothionem. Lipσsomes, an adenovirus vector, or ligand-DNA conjugates can be used to introduce a polynucleotide according to the invention. Administration of the pharmaceutical composition preferably is performed prior to a subject's exposure to an agent. The present method is used durmg treatment of a variety of cancers, including lung cancer, prostate cancer, cervical cancer, endometπal cancer, ovarian cancer and bladder cancer. Other objects, features and advantages of the present invention will become apparent from the following detailed description. It should be understood, however, that the detailed description and specific examples, while indicating preferred embodiments of the mvention, are given by way of illustration only, since various changes and modifications within the spirit and scope of the invention will become apparent to those skilled in the art from this detailed description. Brief Description of the Drawings FIGURES 1A and IB are schematic drawings of the construction of a Metallothionem (MT) recombmant adenovirus vector (Ad-MT) of the present invention. Figure 1A illustrates the Wild-type Adenovirus type 5 (Ad5) genome showing the Ela, Elb and E3 regions and the portion to be deleted from the left end of Ad5 for insertion of the appropriate expression cassettes. An expression cassette is a nucleic acid construct that includes, as operably linked components in the direction of transcription, a transcriptional initiation region, a nucleic acid sequence encoding a protem of interest and a transcriptional termination region wherein the transcriptional regulatory regions are functional m the targeted mammalian host cell. Figure IB illustrates an expression cassette containing regulatory sequences and a recombmant DNA sequence encoding metallothionem. FIGURES 2A and 2B are schematic drawings of the construction of a gamma-glutamyltranspeptidase recombmant adenovirus vector (Ad-γ-GTP) . Figure 2A illustrates the Wild- type adenovirus type 5 (Ad5) genome showing the Ela, Elb and E3 regions and the portion to be deleted from the left end of Ad5 for insertion of the appropriate expression cassettes. Figure 2B illustrates an expression cassette containing regulatory sequences and a recombmant DNA sequence encoding γ-GTP. FIGURES 3A and 3B are schematic drawings of the construction of a manganese superoxide dismutase recombmant adenovirus vector (Ad-MnSOD) . Figure 3A illustrates the Wild- type adenovirus type 5 (Ad5) genome showing the Ela, Elb and E3 regions and the portion to be deleted from the left end of Ad5 for insertion of the appropriate expression cassettes. Figure 3B illustrates an expression cassette containing regulatory sequences and a recombmant DNA sequence encoding manganous superoxide dismutase. Detailed Description of the Invention Ionizing radiation produces toxic free-radical species. Antmeoplastic agents, particularly the class of chemotherapeutic drugs known as alkylating agents, also produce free radicals that are cytotoxic because of their ability to form covalent bonds with nucleic acids. Most alkylating agents, including cyclophosphamide, nitrogen mustard, melphalan, chlorambucil, busulfan, nitrosourea, cis-plat um, streptozotoc , aziπdmylbenzoqumone (AZQ) , dicarbazme (DTIC) , mAMSA and mitoxantrone, form positively charged carbonium ions that yield a charged alkylating intermediate R-CH 2 -CH 2 + that attacks electron-rich sites on nucleic acids, proteins, small molecules and ammo acids. Chabner et al . , in CANCER; PRINCIPLES AND PRACTICE OF ONCOLOGY, 2nd edition, DeVita et al . (eds.) (J.B. Lipp cott Co., Philadelphia 1985). The method of the present mvention provides a means for protecting normal cells, particularly cells of the oral cavity, oropharynx, esophagus, stomach, small intestine and colon, of an individual against an agent that elicits the production of a free radical, a superoxide anion, and/or a heavy metal cation. The cells to be protected may be a site remote from the tumor site. The method of the present invention employs gene therapy, which is the transfer of genetic material into specific cells of a patient . - 6 - Transient gene expression, according to the present invention, can result oy one of two mechanisms. Gene transfer can be used to introduce DNA sequences into the nucleus in an unmtegrated form. In that case, transient expression, or nonmtegrated expression is limited by the stability of the nonmtegrated DNA molecule (s) and may persist for extended periods of time, but rarely persists for periods longer than about one to three weeks. Alternatively, a gene or polynucleotide may be stably integrated into the genome. A gene that is transferred to an individual is called a transgene. The gene therapy method of the present mvention involves an in vivo method of gene therapy that provides a polynucleotide encoding a protem capable of neutralizing or eliminating a toxic free radical, superoxide anion and/or heavy metal cation, wherein the protem is transiently expressed the individual. The transgenes of the present invention encode protem(s), such as metallothionem, superoxide dismutase or gamma glutamyl transpeptidase, that scavenge a toxic free radical, superoxide anion and/or heavy metal cation. γ-Glutamyltranspeptidase (γ-GTP) is a plasma membrane- associated ectoenzyme that catalyzes the transpeptidation of extracellular glutathione into ammo acid intermediates, which are then transported across the cell membrane and used to resynthesize glutathione de novo . Glutathione (GSH) detoxifies free-radicals. Cells generally synthesize GSH de novo from the constituent ammo acids. A cell's sensitivity to radiation is directly correlated with its ability to transpeptidate extracellular glutathione via γ-GTP. Cell lines with high γ-GTP activity are more resistant to the effects of radiation and are more capable of repairing damage induced by low doses of γ-irradiation than cell lines with low γ-GTP activity. See Examples 7 and 8. Tumor cells depleted of GSH have been shown to be more susceptible to ionizing irradiation and chemotherapeutic agents because GSH-dependent detoxification pathways are reduced. Louie et al . , Cancer Res . 45: 2110 (1985). Protection against superoxide radicals requires antioxidants, such as GSH, and the 0 2 -scavenging enzyme superoxide dismutase (SOD) . SODs are metalloenzymes that are - 7 - essential for dismutation of 0 2 " to H 2 0 2 and 0 2 . There are three forms of SODs : copper-zinc (CuZnSOD) , manganous (MnSOD) and iron (FeSOD) . Although CuZnSOD and FeSOD are made constituitively, MnSOD synthesis is mducible. Induction of MnSOD activity has been shown to follow X-irradiation of heart tissue. Oberley et al . , Arch . Biochem . Biophys . 254: 69 (1987). Further, hematopoietic tumor cell lines transfected with MnSOD cDNA in vi tro demonstrate increased resistance to radiation. Suresh et al . , Experimental Hema tology 21: 1828 (1993). Metallothionems are low molecular weight proteins consisting of a single polypeptide chain of 61 ammo acid residues, of which 20 are cystemes that chelate cations. Induction of metallothionem has been shown to provide resistance to ionizing irradiation damage. Metallothionem protem protects cells from the toxic effects of heavy metal ions and is a powerful scavenger of radiation-induced OH-radicals in vi tro . Cells lines that express high levels of MT are resistant to DNA damaging agents, such as cis-platmum and chlorambucil , and ionizing radiation. Andrews et al . , Cancer Chemother. Pharmacol . 19: 149 (1987); Bakka et al . , Experientia 38: 381 (1982); Matsubara et al . , Environ . Res . 43: 66 (1987). Metallothionem is capable of scavenging free radicals produced by electrophilic anticancer drugs and ionizing radiation in vi tro . Endresen et al . , Cancer Res . 43: 2918 (1983); Thornalley et al . , Biochim . , Biophys . Acta 827: 36 (1985). Importantly, induction of MT m mouse liver provides protection against lethal damage from high dose radiation. Matsubara et al . , Rad . Res . Ill: 267 (1987). Nonetheless, some cell lines transfected with the MT gene in vi tro were as sensitive to ionizing radiation and bleomycm as non-transfected recipient cells. However, MT transfected cells were resistant to mitomycm, suggesting that MT protem protects some cells in vi tro from monofunctional alkylating and cross- linking agents but not from free radicals. Lohrer et al . , Carcmogenesis 10: 2279 (1989). A DNA sequence encoding an entire superoxide dismutase, preferably MnSOD, coding region can be isolated or synthesized by methods well known to the art based on the MnSOD sequences reported by Oursler et al . , J. Cell . Biochem . 46: 219 (1991) or Beck et al., Nucl . Acids. Res. 15: 9076 (1987), or the SOD sequences reported by U.S. Patent No. 4,751,180; Lieman-Hurwitz et al., Proc. Natl . Acad. Sci . USA 79: 2808 (1982); U.S. Patent No. 4,742,004; Xiang et al . , Nucleic Acids Res. 15: 7654 (1987) or Sherman et al . , Proc. Natl. Acad. Sci. USA 80: 5465 (1983), the contents of each of which are incorporated by reference in their entirety. Alternatively, these sequences can be prepared by the polymerase chain reaction by methods well known to those of skill in the art. See, e.g., Wong et al . , Cell 58: 923 (1989). DNA sequences encoding various species and isoforms of metallothionem can be isolated or synthesized by methods well known to the art based on the sequences reported for human MT by [Yamazaki et al . , Biochem Int. 28: 451 (1992); Soumillion et al., Eur. J. Biochem. 209: 999 (1992); Karin et al., Proc. Natl. Acad. Sci. USA 80: 4040 (1983); Paliwal et al . , Neurochem. Int. 17: 441 (1990); Schmidt et al . , J. Biol . Chem 260: 7731 (1985); Richards et al., Cell 37: 263 (1984); Karin et al., Nature 299: 797 (1982); Hyland et al . , Nucleic Acids Res . 15: 1350 (1987);, sheep and mouse [Peterson et al . , Eur. J. Biochem. 160: 579 (1986)], fish [(Lee et al . , Korean Biochem J. 25: 48 (1992); Bonham et al., DNA 6: 519 (1987)] and insect [Lastowski-Perry et al . , J. Biol. Chem. 260: 1527 (1985)], the contents of each of which are incorporated by reference in their entirety. Preferably, the human metallothionem sequences disclosed by either Yamazaki et al., (1992) supra, or Soumillion et al . , (1992) supra are used in the method of the present mvention. DNA encoding γ-GTP can be provided for use in the present invention by isolating or synthesizing such a sequence by methods well known to the art based on the sequences reported by any of Altman et al . , Biochemistry 32: 3822 (1993); Ishiye et al., Biotech. Progr. 9: 323 (1993); Ishiye et al, FEMS Mirobiol. Lett. 97: 235 (1992); or Angele et al . , Clin. Chem. 37: 662 (1991) , the contents of each of which are hereby incorporated by reference. DNA encoding MT, SOD, MnSOD, or γ-GTP can be provided for use m the present invention by methods well known to those of skill in the art, such as (1) oligonucleotide synthesis of the desired DNA sequences based on the sequences disclosed in the above recited references; (2) isolation of the desired DNA sequences from the plasmids disclosed in the above references or from plasmids available from American Type Culture Collection (ATCC) (12301 Parklawn Drive, Rockville, Maryland 20852) such as: [a] ATCC 57117 - pHM6 containing the human metallothionem 2 pseudogene 1; [b] ATCC 57152, 57153 - bMT-IIA containing the human metallothionem 2 gene; [c] ATCC 20745 - pYASll containing cDNA encoding human superoxide dismutase 1; [d] ATCC 20796 - pYLUIGF2-14 containing DNA encoding human superoxide dismutase 1 ; [e] ATCC 39786 - pSOD alpha 2 containing DNA encoding human superoxide dismutase 1; [f] ATCC 59946, 59947 - phMnSOD4 containing DNA encoding human superoxide dismutase 2 ; [g] ATCC 61646, 61647 containing cDNA encoding human superoxide dismutase 1; [h] ATCC 86406 - IB881 containing cDNA encoding human superoxide dismutase or (3) polymerase chain reaction amplification of the desired DNA sequences from the DNA libraries disclosed m the above references using primers based on the sequences disclosed m the recited references. Transient expression of genes administered m vivo is viewed in this art as a major technical limitation to gene therapy. See Mulligan, Science 260: 926 (1993). In sharp contrast, according to the present invention transient expression of the genes is highly desirable because protection of the normal tissue is needed only for the period of radiation therapy or chemotherapy; thereafter, rapid clearance of the gene product is desirable. Transient expression is desirable because the prolonged clinical effects of elevated MT, γ-GTP and/or MnSOD are unknown. Also, clearing of the transgene and its vector may be clinically desirable after chemotherapy or radiation therapy to provide for the next phase of a combined modality therapeutic approach. The methods of the present mvention are designed to result in transient or nonmtegrated expression of an exogenous gene m vivo; however, in the event that a limited amount of stable integration of the exogenously provided DNA also results, the method of the present invention remains functional in its ability to provide a protem capable of neutralizing or eliminating a toxic ionic species in vivo . Transient expression can be achieved by directed introduction of the genetic material encoding the desired - 10 - proteins into cells or by use of a heterologous virus genome as a vector. Methods for delivering genes into mammalian cells to provide transient expression that can be utilized for gene therapy include: papovaviruses , adenovirus, vaccinia virus, herpesviruses, poxviruses, polio virus, smdbis and other RNA viruses, ligand-DNA conjugates, adenovirus-ligand-DNA conjugates, naked DNA, lipofection and receptor-mediated gene transfer. See, eg. , Mulligan, supra. Coen in VIROLOGY, Fields et al . (eds.) Raven Press, Ltd., (New York, 1990); Ferkol et al . , FASEB 1 -. 1081 (1993) . Animal model studies have efficiently transferred genes using retroviruses (Friedmann, Science 244: 1275 (1989)), adenoviruses (Rosenfeld et al . , Science 252: 431 (1991); Rosenfeld et al . , Cell 68: 143 (1992)) and liposomes (Feigner et al . , Nature 349: 351 (1991). Transient expression is achieved by virtue of an mducible transcriptional promoter to control the expression of the gene or polynucleotide. In a preferred embodiment, the mducible promoter is induced directly or indirectly by the ionizing radiation therapy or chemotherapy agent itself. A suitable promoter is the ergl promoter, a promoter induced by irradiation. Hallahan et al . , Proc . Na tl . Acad . Sci . USA 88: 2156-2160 (1991) and Datta e al., Proc. Natl . Acad . Sci . USA 89: 10149-10153 (1992). Transcription of polynucleotide (s) or gene(s) controlled by erg-1 stops somewhere between 60 and 90 hours post induction. The method of the present invention can be used to protect specific tissues in cancer patients against the damaging effects of ionizing radiation and chemotherapeutic drugs, which produce free radicals, superoxide anions, and/or heavy metal cations. In particular, the method of the present invention can be used to transfer a gene to normal cells at a site remote from the tumor site prior to clinical radiation therapy or chemotherapeutic drug administration to combat cancer. In particular, the method of the present mvention can be used to transfer a gene to normal cells of the oral cavity, oropharynx, esophagus, stomach, small intestine or colon prior to clinical radiation therapy or chemotherapeutic drug administration to 11 - combat lung, prostate, bladder, cervical or endometrial cancer, for example. In one preferred embodiment, the method of the present invention is directed toward transient in vivo gene therapy to lung cancer patients to provide protection of the oral, cavity, oropharynx and esophagus when the lung cancer is treated with ionizing radiation therapy or anti-neoplastic alkylating agents. A limiting factor m the treatment of lung cancer, particularly with treatments regimens that entail a combination of radiation therapy and chemotherapy with paclitaxel, vmblast e, or cαs-platinum, has been the development of esophagitis durmg the radiation treatments. In some cases, patients cannot complete a course of therapy because of the development of severe esophagitis. The esophagitis is the result of injury to the mucosa layer of the esophagus. After irradiation, there is an increase m the mitotic activity m the mucosa to repair the damage. Paclitaxel, in particular, works by blocking mitosis during G 2 /M, the portion of the cell cycle which is the most radiosensitive. It is hypothesized that the combination of the inhibition of mitosis by the chemotherapeutic agent and the sensitization of the mucosal cells during irradiation prevents repair of mucosal damage and causes the esophagitis. In another embodiment, the method of the present invention is directed toward transient m vivo gene therapy to prostate, bladder, cervical or endometrial cancer patients to provide protection to the small intestine and colon when these cancers are treated with ionizing radiation therapy or anti- neoplastic alkylating agents. The mechanism is the same as that described above for esophageal protection. In this case, it is hypothesized that the combination of inhibition of mitosis by the chemotherapeutic agent and the sensitization of the mucosal cells during irradiation prevents repair of mucosal damage to intestinal crypt cells and colon crypt cells. In addition to protection of normal cells at a site remote from the tumor site prior to clinical radiation therapy or chemotherapeutic drug therapy, the method of the present invention also is useful to protect normal cells of the oral cavity, oropharynx, esophagus, stomach, small intestine and colon - 12 - during treatment of a tumor in the same region. In this case, the transgene is delivered and expressed more efficiently in normal tissue as compared to tumors of such tissue. More efficient expression in normal tissue than in tumor tissue has been confirmed by studies in experimental animals, e . g. , rats or mice. A lower expression of the transgene in tumor cells transplanted into the animals as compared to the normal surrounding tissue confirms a lower ratio of delivery of therapeutic genes to tumor cells than normal cells targeted for protection. Compositions for use in the present method comprise a polynucleotide that encodes a protein that is transiently expressed in a subject when the subject is exposed to an agent that elicits production of a toxic species, such as a free radical, a superoxide anion, or a heavy metal cation, wherein the protein is capable of neutralizing or eliminating the toxic species; and a pharmaceutically acceptable vehicle for the polynucleotide. In this context a pharmaceutically acceptable vehicle is inert or otherwise medically acceptable, and is compatible with the active agent, in a particular context of administration. In addition to a suitable excipient, a pharmaceutically acceptable carrier can contain conventional additives like diluents, adjuvants, antioxidants , dispersing agents and emulsifiers, anti- foaming agents, flavor correctants, preservatives, solubilizing agents and colorants. More particularly, pharmaceutically acceptable vehicles are characterized by having physiologically acceptable pH and ionic strength. Sterile, buffered saline, particularly phosphate- buffered saline, is a preferred vehicle for compositions to be administered parenterally . A preferred embodiment uses artificial lipid membranes (i.e., liposomes) for delivery. Procedures for introducing DNA into cells that employ lipid include: polyethylene glycol to mediate fusion of protoplast derived from plasmid-containing bacteria (Schaffner, Proc. Natl . Acad . Sci . USA 11 : 2163 (1980); D A-containing erythrocyte ghosts (Wiberg et al . , Nucleic Acids Res . 11: 7287 (1983); DΝA-containing liposomes (Fraley et al . , Proc . Natl . Acad . Sci . USA 76: 3348 (1979); plasmid/cationic 13 liposome complexes (Striblmg et al . , Proc . Natl . , Acad. Sci . USA 89: 11277 (1992); W093/12756) and lipofection (Feigner et al . , Proc. Natl . Acad Sci . USA 84: 7413 (1987). The gene therapy method of the present mvention can employ any of the above procedures for introducing genetic material into cells in vivo, but lipofection with plasmid/cationic liposome complexes is a preferred method. Lipofection employs a liposome formulation of catiomc lipid to transfect nucleic acids into cells. The lipid-nucleic acid complex fuses with plasma membranes and transfers the nucleic acid into the cells efficiently, where the DNA is expressed. Lipofection is five to one hundred times more efficient introducing DNA into cells than calcium phosphate or DEAE-dextran transfection methods. Chang et al . , Focus 10: 66 (1988) . Liposome preparations can be prepared as described m the art or purchased from commercially-available sources, such as GIBCO BRL's lipofect (GIBCO BRL, Life Technologies, Inc., P.O. Box 9418, Gaithersburg, Maryland 20898). Feigner et al . , (1987) supra ; Schreier, J. of Liposome Res . 2 : 145 (1992) ; Chang et al . , (1988) supra . Transient transfection employing lipofection is measured 24 to 72 hours after transfection by assays that measure gene expression of the transfected gene(s) . Commonly used assays monitor enzyme activities of chloramphenicol acetyltransferase (CAT) , LAC-Z, /3-galactosιdase, luciferase, or human growth hormone that can be contained in the constructs . Using lipofection, human small cell lung cancer cells have been transiently transfected. Chang et al . supra . Lipofection of DNA encoding MT, CuZnSOD, FeSOD, MnSOD and γ-GTP encoding DNA to any target tissue can be performed using lipofection techniques well known to those of skill in the art. A preferred preparation of a catiomc lipid preparation is composed of 1:1 DOTMA or DDAB/DOPE (i.e., 1:1 of N-[l-(2,3- dioleyloxy) propyl] -N, N, N-triethylammonium (DOTMA) or dimethyldioctadecyl-ammoniumbromide (DDAB) and cholesterol and dioleoyl phosphatidylethanolamme (DOPE). To produce 1:1 DOTMA/DOPE catiomc liposomes, stock solutions of lipids are dissolved in chloroform and stored under argon at -20°C. Lipids - 14 are mixed m round bottomed flasks and evaporated to dryness on a rotary evaporator under reduced pressure. Final lipid concentrations of lOmM each are made by adding double-distilled water. The resulting mixture is sonicated to produce a suspension of liposomes. Plasmid is complexed to DOTMA/DOPE liposomes as follows. Plasmid DNA and DOTMA/DOPE liposomes are diluted separately in water prior to being mixed. The volume of water may range between 1 and 20 ml, preferably about 8 ml. The composition of the liposome-DNA complex may range from about 4:1 to about 1:10 micrograms DNA to nanomoles of catiomc lipid, preferably from about 1:1 to 1:2 micrograms DNA to nanomoles of catiomc lipid. Alternatively, to specifically transfer DNA capable of expressing γ-GTP, MT, SOD and/or MnSOD in a desired/particular human target tissue m vivo, replication-deficient recombmant adenoviruses can be used. For example, Ad.CMV-lacZ (containing cytomegalovirus) and Ad. CB-MnSOD viruses, which are based on adenovirus type 5 (Ad5) and produced by homologous recombination m transformed primary human embryonal kidney cell line 293 (ATCC Catalogue Number CRL1573) , can be used m the method of the present invention. Graham et al . , METHODS IN MOLECULAR BIOLOGY (Murray, Humana, 1991). To construct a recombmant adenovirus according to the present invention, approaches well known to those of ordinary skill in the art can be utilized. For example, a recombmant adenovirus of the present invention can be constructed from an adenovirus type 5 (Ad 5) deletion mutant, such as Ad-dl324 (Thimmappaya et al . , Cell 31: 543 (1982)) and a plasmid containing the Ad5 5' inverted terminal repeat, origin of replication, encapsidation signal, Ela enhancer, the major late promoter, the tripartite leader sequence cDNA and the DNA sequence encoding the entire protem sequence of human MT, γGTP or MnSOD and the SV40 early polyadenylation signal. The recombmant vectors Ad-MT, Ad-γ-GTP, and Ad-MnSOD are constructed by deleting the majority of the E3 region and 2.6 mu from the left end of Ad5 and adding to the left end the MT, γ-GTP or MnSOD expression cassettes, which contain the regulatory sequences and the recombmant MT, γ-GTP or MnSOD encoding DNA. The left end of 15 the viral genome, including the Ela and the majority of the Elb region is deleted and replaced by the MT or γGTP or MnSOD expression cassette containing the essential viral cis-acting elements, including the inverted terminal repeat, an origin of replication, the encapsidation signal, the Ela enhancer and no Ela structural gene. Preferably, the Ela enhancer is followed by the adenovirus type 2 major late promoter and cDNA encoding the MT, γGTP or MnSOD. The constructed recombmant adenovirus is then replicated m a permissive cell line that contains a functional Ela gene to provide a trans-actmg Ela protem, such as the 293 human kidney cell line. Thereafter, high titer, infectious recombmant adenoviral stocks are prepared. Another way to produce a recombmant adenoviral vector is to coprecipitate a linearized plasmid containing the desired cDNA encoding MT, γ-GTP or MnSOD with the large fragment of compatibly cut Ad-dl324 DNA using the calcium-phosphate precipitation method. Graham et al . , Virology 52: 456 (1973); Wigler et al . , Cell 14: 725 (1978) . The co-precipitated DNAs are then cotransfected into 293 cells to allow homologous recombination to occur. Recombmant adenovirus DNA is tranfected into 293 cells (Graham et al . , J. Gen . Virol . 35: 59 (1977); Graham et al . , Virology (1973), supra) where it is replicated, encapsidated into an infectious virus and isolated by plaque purification. Individual plaques are amplified by propagation in 293 cells and viral DNA is extracted. Hirt, J. Mol . Biol . 26: 365 (1967) . Recombmant adenovirus plaques containing the human gamma glutamyl transpeptidase, manganese superoxide dismutase and metallothionem prote cDNA (Ad-γGTP; Ad-MnSOD, and Ad-MT respectively) then are identified by restriction cleavage, Southern analysis and/or Northern analysis using the appropriate DNA probes. Control virus having a deletion of the Ela region and not containing the DNA of interest will not demonstrate detectable γ-GTP, MnSOD or MT transcripts m a Northern analysis whereas constructs containing the DNA of interest will demonstrate a detectable γ-GTP, MnSOD or MT transcript. Each of Ad-γ-GTP, Ad-MnSOD, and Ad-MT vectors are propagated m 293 cells and recovered 36 hours after infection by - 16 - several cycles of freeze/thawing. All viral preparations are purified by CaCl 2 density centπfugation, dialyzed and stored in virus dialysis buffer (10 mM Tris-HCl, pH 7.4, 1 mM MgCl 2 ) at 4°C for immediate use, or frozen at -70°C with the addition of 10% glycerol . The titer of the viral stock is determined by plaque assays using 293 cells. Any tissue of the human body can be targeted for the gene therapy of the present invention using the adenoviral vectors described above. For evaluation of MT, γ-GTP or MnSOD mRNA, or protein synthesis or the evaluation of functional protem, the recombmant vector is used to infect either 293 cells or rat respiratory epithelial cells. To obtain rat respiratory epithelial cells, rats are sacrificed, the lungs and trachea are isolated. Cells are obtained by cytologic brush (Rosenfeld et al . , supra (1991) plated, and infected with 2 X IO 7 plaque forming units (PFU) of Ad-MT, Ad-γ-GTP, or Ad-MnSOD media, or, as a control, exposed to only media. According to the present mvention, conditions are established for achieving recombmant gene expression m a majority of the cells of the target organ to be protected in vivo . It may not be necessary to achieve greater than 50% transgene expression or even greater than 10% transgene expression if cell-to-cell protection is involved m the transfected organ. For example, one transgene expressing cell may be able to protect ten non-transfected cells m a local niche by cell-to-cell transfer of intermediates (e.g., one nucleotide or one nucleoside) involved in the cellular repair cascade. Dosages of the pharmaceutical compositions administered according to this invention are generally known m the art. The dosage of liposome-DNA complex may range from about 5-50 mg plasmid per 5 to 100 μmoles of liposomes, preferably about 12 mg plasmid per 24 μmoles liposome. Preparations using adenovirus according to the invention are dispensed in dosage unit form comprising between IO 6 and IO 14 PFU/ml of viral vector in a pharmaceutically acceptable carrier per unit dosage, preferably about 10 10 to 5 X 10 13 PFU/ml of the replication-deficient adenovirus Ad-γ-GTP, Ad-MnSOD and/or Ad-MT. The desired pfu are contained in a total volume of between 0.3 and 2.0 ml of 17 - phosphate buffered saline (PBS) and administered by techniques known to one skilled m the art. When a ligand-DNA complex is utilized to deliver the desired gene to the target cells, the ligand conjugate is complexed to plasmid DNA using a molar ratio of carrier to DNA of between approximately 10:1 and 500:1, preferably between 300:1 and 500:1. For protection of the oral cavity, oropharynx, esophagus, stomach and small intestine, compositions for use in the method preferably are administered orally. For example, protection of the oral cavity, oropharynx and esophagus, is achieved by having patients swallow plasmid liposome complex daily before radiotherapy. Typical therapy to tumors m the chest comprises 30-35 radiation treatments over 6%-7% weeks. For lung cancer, patients typically receive between 6000 and 7000 cGy of irradiation to the lung cancer tumor volume. For protection of the stomach and small intestine, slow-release formulations that are encapsulated with an enteric coating are used. The enteric coating is designed to release the active complex in the organ to be protected. For protection of the colon, the composition preferably s administered by enema or by fiber-optic colonoscope entry into the colon up to the cecum. Generally this treatment is not given daily, but every other day or three times a week, especially m the case of administration by fiber-optic colonoscope . The following examples illustrate specific embodiments according to the present invention, but do not limit the scope of the mvention in any way. Further aspects and variations of the invention, based on the disclosure above and the following examples, will be apparent to the person of ordinary skill m the art. Example 1 : Construction of recombmant adenoviral vectors Ad-MT, Ad-MnSOD and Ad-Υ-GTP The adenovirus major late promoter is linked to a recombmant human MT gene (Yamazaki et al . , supra ; Soumillion et al . , supra) and is incorporated into a replication-deficient recombmant. Straus m THE ADENOVIRUSES, Ginsberg (ed.) (Plenum Press, New York 1984); Gilardi et al . , FEBS Lett . 267: 60 (1990). - 18 - The vector has a deletion of part of the E3 region and part of the viral Ela coding sequence, yet contains an insert of an MT expression cassette (FIGURES 1A and IB) . Ad-MT is constructed by deleting the majority of the E3 region and a portion of the left end of Ad5 and adding to the left end of the MT expression cassette from a plasmid containing the nucleic acid sequence encoding MT. The adenovirus major late promoter is linked to a recombmant human γ-GTP gene (Altman et al . , Biochemistry 32: 3822 (1993) and incorporated into a replication-deficient recombmant. Straus, supra; Gilardi et al . , supra . The vector has a deletion of part of the E3 region and part of the viral Ela coding sequence, yet contains an insert of an γ-GTP expression cassette (FIGURES 2A and 2B) . Ad-γ-GTP is constructed by deleting the majority of the E3 region and a portion of the left end of Ad5 and adding the left end of the γ-GTP expression cassette from a plasmid containing the nucleic acid sequence encoding γ-GTP. The adenovirus major late promoter is linked to a recombmant human MnSOD gene (Beck et al . , Nucl . Acids . Res . 15: 9076 (1987)) and is incorporated into a replication-deficient recombmant. Straus, supra ; Gilardi et al . , supra . The vector has a deletion of part of the E3 region and part of the viral Ela coding sequence, yet contains an insert of an MnSOD expression cassette. Ad-MnSOD is constructed by deleting the majority of the E3 region and a portion of the left end of Ad5 and adding to the left end of the MnSOD expression cassette from a plasmid containing a nucleic acid sequence encoding MnSOD (FIGURES 3A and 3B) . In each case, once the expression cassette is packaged into an infectious, replication-deficient virus, recombmant vector is capable of directing the synthesis of human MT, human γ-GTP, human MnSOD, respectively, n vi tro in 293, CHO and HeLa cell lines. Gilardi et al . , supra . Expression is confirmed by functional assays. - 20 pHB APr-1 is entrapped m the sonicated liposome suspension by extensively vortexmg 0.5 ml of DNA solution with the sonicated suspension for 1 minute followed by three cycles of freezing and thawing. DNA-entrapped liposomes are separated from the non- entrapped DNA by gel filtration on a Sepharose 4B column diluted with PBS. The amount of liposomes (30-40 μg) and the amount of DNA (1 to 5 μg) is optimized for cell type based on a dose response curve to determine cell toxicity. Feigner et al . , (1989) supra . The amount of liposome used for lipofection is about 50% of its toxic concentration. To prepare plasmid/cationic liposome complexes, catiomc liposomes are made containing catiomc lipid preparation of 1:1 DOTMA/DOPE (i.e. 1:1 of N- [1- (2 , 3 -dioleyloxy) propyl] -N,N, - triethylammonium (DOTMA) and dioleoyl phosphatidylethanolamme (DOPE) . Stock solutions of lipids are dissolved in chloroform and stored under argon at -20 °C. Lipids are mixed in round bottomed flasks and evaporated to dryness on a rotary evaporator under reduced pressure. Final lipid concentrations of lOmM each are made by adding double-distilled water. The resulting mixture is sonicated for about 20 minutes. Between 5 and 50 mg, preferably 12 mg of plasmid is complexed to between 5 and 100 μmol , preferably 24 μmol of DOTMA/DOPE liposomes. LacZ plasmid/liposome complexes are prepared as controls. Example 4 • In vi vo expression of MT, MnSOD and/or Ύ-GTP following lipofection with plasmid/liposome complexes Mice are transfected using lipofection with DNA encoding MT, MnSOD, γ-GTP, or LacZ plasmid/liposome complexes prepared according to Example 3. Adult C3H/HeNsd mice of approximately 12 weeks of age are injected with the plasmid/liposome complex by passing a tube attached to a 28 gage needle through the oral cavity and depositing 150 μl of plasmid/liposome complex comprising 1 mg of either MT, MnSOD, γ-GTP, or LacZ plasmid DNA (10 μg/ml) , and 56 μl of lipofectant at the top of the esophagus. A solution of naked-DNA, at the same concentration as that used in the liposome preparation, serves as a control. - 19 - Example 2 : In vivo expression of MT, -y-GTP and MnSOD following transfection with recombmant adenoviral vectors Ad-MT, Ad-Υ-GTP, and/or Ad-MnSOD Ad-MT, Ad-γ-GTP, or Ad-MnSOD, or a combination of (a) Ad-γ-GTP and Ad-MT, or (b) Ad-γ-GTP and Ad-MnSOD, or (c) Ad-MT and Ad-MnSOD or (d) each of Ad-MT and Ad-MnSOD and Ad-γ-GTP, is used to transfect esophageal tissue of C3H/HeNsd mice m vivo . The mice are injected by passing a tube attached to a 28 gage needle through the oral cavity and depositing recombmant vector at the top of the esophagus. A solution of naked-DNA, at the same concentration as that used in the active preparation, serves as a control. The mice are sacrificed 24 hours later, and the esophagus removed. The tissue is tested for: (1) immunoreactive MT, MnSOD, or γ-GTP, as measured by immunoprecipitation or Western blotting, and (2) functional MT, MnSOD and/or γ-GTP activity. Human transcripts of the proteins are observed m the transfected tissue; SDS-PAGE and autoradiography of protem samples from biopsied tissue reveal de novo expression of a 6,000 dalton human MT, a 16,000 to 19,000 dalton human MnSOD, and a 62,000 dalton human γ-GTP. No expression is observed in mice that received a solution of naked DNA. Example 3 : Preparation of MT, MnSOD and/or γ-GTP lipid carrier-nucleic ac d complexes Lipid carrier-nucleic acid complexes are prepared by methods well known in the art, such as those disclosed by Debs et al . , W093/12756 or Stπblmg et al . , supra , the entire contents of which is incorporated by reference herein. Alternatively, liposomes for lipofection can be produced as follows or purchased from GIBCO BRL. To prepare liposomes for lipofection, 20 mg of egg phosphatrolycholme is rotary evaporated with a vacuum drier from a chloroform solution to form a thin film on the walls of a 5 ml round-bottomed flask for 1 hour. The dried thin film lipid is suspended in 0.5 ml phosphate buffered saline (PBS) pH 7.4 on a vortex mixer and then sonicated. An expression vector comprising DNA encoding MT, MnSOD and/or γ-GTP and a promoter, such as human beta-actm promoter in - 21 - The mice are sacrificed 24 hours later, and the esophagus removed. The tissue is tested for: (1) immunoreactive MT, MnSOD, or γ-GTP, as measured by immunoprecipitation or Western blotting, or LacZ, as measured by staining, and (2) functional MT, MnSOD and/or γ-GTP activity. Human transcripts of the proteins are observed m the transfected tissue; SDS-PAGE and autoradiography of protem samples from biopsied tissue reveal de novo expression of a 6,000 dalton human MT, a 16,000 to 19,000 dalton human MnSOD, and a 62,000 dalton human γ-GTP. LacZ expression is confirmed m mice that received LacZ plasmid/liposome complexes. No expression is observed m mice that received a solution of naked DNA. Example 5 • Protection of esophagus from ionizing radiation and alkylating agents Control mice and mice that transiently express recombmant MT, MnSOD, γ-GTP or a combination of these proteins in esophageal tissue, as a result of lipofection with plasmid/liposome complexes according to Example 4, are tested to see whether the recombmant protem (s) protect the esophagus during irradiation. A first group of mice is exposed to ionizing radiation to the lung with a dose of hemi-body irradiation delivering about 1800 to 2500 cGY m one fraction or about 2000 to 3000 cGY m multiple fractions. These regimens produce acute radiation esophagitis in unprotected animals within two to three days of the single fraction delivery. At serial time points after irradiation between about one day and two weeks after irradiation, the animals are sacrificed, and the irradiated and control esophagus are removed. The effect of transgene expression on the development of chemoradiation- induced esophagitis also is assessed. A second group of mice is exposed to a chemoradiation treatment using taxol, to determine whether increased expression of MT, MnSOD and/or γ-GTP protects against the development of esophagitis resulting from chemoradiation therapy. In this case, the mice receive a single dose of 6 mg/kg taxol or a fractionated dose of 1.5 mg/kg/day over 5 days of taxol via mtraperitoneal injection. On the last day of taxol injection, the experimental mice receive - 22 - an esophageal injection of MT, MnSOD or γ-GTP plasmid/liposome complex. Control and experimental mice are irradiated with 1500, 1750 or 2000 cGy. A mouse model for irradiation-induced esophagitis has been described previously. Rozenzweig et al . , Nucl . Med. Biol . , 21: 171-178 (1994) . According to the model, LD50/30 was used to describe mortality with deaths due to esophagitis occurring within the first 30 days following irradiation. C3H/HeJ mice were shown to have LD50/30 of 1900 cGy, which was decreased to 1252 or 1686 cGy when adriamycin was administered 1 or 7 days in advance, respectively. The mouse model for esophagitis is used to assess mortality from esophagitis caused by radiation-induced changes, by comparing LD50/30 in control and experimental mice. In order to determine LD50/30, mice are irradiated 24 hours after injection with plasmid/liposome complex with a single dose of irradiation ranging from 1800 to 2500 cGy. The mice are shielded such that the irradiation is restricted to the pulmonary cavity. The mice are weighed before irradiation, and at 7 , 14, 21 and 28 days after irradiation. The mice are observed daily and are sacrificed if they lose more than 20% of their body weight or experience difficulty breathing or moving. After 30, days, all the remaining mice are sacrificed. A fractionated irradiation schedule also is used. In this case, the mice receive an intraesophageal injection of plasmid/liposome complex 24 hours before the first dose and every 48 hours thereafter. The irradiation dose is fractionated as 400 cGy x 5, 300 cGy x 10, or 250 cGy x 12. Esophagitis also is monitored by following its histological progression. Mice are sacrificed at serial points following treatment, and the esophagus is removed, fixed in formalin, sectioned and stained it with hematoxylin and eosin. The sections are examined under a microscope for alterations in the mucosal layer, which are scored using Optimas Image Analysis software to quantitate damage to the esophagus. Values for LD50/30 are significantly lower in mice that receive intraesophageal injections of plasmid/liposome complex that contains MT, MnSOD or γ-GTP. In addition, histological - 23 - examination of the esophagus demonstrates that the complexes effectively prevent the damage to the mucosal layer that is associated with irradiation- induced esophagitis. While the invention has been described in detail with respect to particular preferred embodiments, it should be understood that such description is presented by way of illustration and not limitation. Many changes and modifications within the scope of the present invention may be made without departing from the spirit thereof, and the invention includes all such modifications.

Claims: - 24 - What Is Claimed Is 1. A method for protecting the oral cavity, oropharynx, esophagus, stomach, small intestine or colon in a subject, comprising administering in vivo a protective pharmaceutical composition to a subject requiring protection of the oral cavity, oropharynx, esophagus, stomach, small intestine or colon from an agent that elicits production of a toxic species selected from the group consisting of a free radical, a superoxide anion, and a heavy metal cation, said pharmaceutical composition comprising (A) a polynucleotide that encodes a protein that is transiently expressed in said subject, wherein said protein is capable of neutralizing or eliminating said toxic species; and (B) a pharmaceutically acceptable vehicle for said polynucleotide. 2. The method of claim 1, wherein said agent is ionizing radiation. 3. The method of claim 2, wherein said ionizing radiation is clinical radiation therapy. 4. The method of claim 1, wherein said agent is a chemotherapeutic drug. 5. The method of claim 1, wherein said polynucleotide is a cDNA and said vehicle is a liposome. 6. The method of claim 1, wherein said polynucleotide is a cDNA and said vehicle is an adenovirus vector. 7. The method of claim 1, wherein said protein is selected from the group consisting of gamma glutamyl transpeptidase, manganese superoxide dismutase, and metallothione . 8. The method of claim 1, wherein said protein is gamma glutamyl transpeptidase. - 25 - 9. The method of claim 1, wherein said protein is manganese superoxide dismutase. 10. The method of claim 1, wherein said protein is metallothionem . 11. The method of claim 1, wherein said pharmaceutical composition comprises a mixture of polynucleotides selected from the group consisting of a polynucleotide encoding gamma glutamyl transpeptidase, a polynucleotide encoding manganese superoxide dismutase and a polynucleotide encoding metallothionem. 12. The method of claim 1, wherein said polynucleotide is under control of an inducible transcriptional regulatory sequence . 13. The method of claim 1, wherein said polynucleotide is under control of a radioinducible transcriptional regulatory sequence . 14. The method of claim 1, wherein said subject is a lung cancer patient requiring protection of tissues of the oral cavity, oropharynx, and esophagus. 15. The method of claim 1, wherein said subject is a patient, with cancer in the lower abdomen, requiring protection of tissues of the colon and small intestine. 16. The method of claim 15, wherein said cancer is cervical cancer. 17. The method of claim 15, wherein said cancer is prostate cancer. 18. The method of claim . 15, wherein said cancer is endometrial cancer. - 26 - 19. The method of claim 15, wherein said cancer is ovarian cancer. 20. The method of claim 15, wherein said cancer is bladder cancer. 21. A method for protecting the oral cavity, oropharynx, esophagus, stomach, small intestine or colon in a subject needing said protecting, said method comprising administering in vivo, at a site remote from a site to be treated by said agent, a protective pharmaceutical composition to said subject, wherein said subject requires protection of the oral cavity, oropharynx, esophagus, stomach, small intestine or colon from an agent that elicits production of a toxic species selected from the group consisting of a free radical, a superoxide anion, and a heavy metal cation, and wherein said pharmaceutical composition comprises (A) a polynucleotide that encodes a protein that is transiently expressed in said subject, said protem being capable of neutralizing or eliminating said toxic species; and (B) a pharmaceutically acceptable vehicle for said polynucleotide.

Inventors: GREENBERGER JOEL S, GREENBERGER, JOEL, S.
Applicants: GREENBERGER JOEL S, UNIV PITTSBURGH, GREENBERGER, JOEL, S., UNIVERSITY OF PITTSBURGH
Application year: 1999
Main classification: A61K48
IPC codes: A61K38, A61K48, A61P1, A61P35, A61P43