INTRINSICALLY BACTERICIDAL ABSORBENT DRESSING AND METHOD OF FABRICATION

Abstract: A superabsorbent polymer dressing having antimicrobial properties for use in fabricating wound dressings, sanitary napkins, tampons and the like, includes a synthetic polymer matrix fabricated to have an enhanced surface area. Antimicrobial compounds are coupled to the surface of the polymer matrix by non-siloxane bonds.

Description: Intrinsicaily Bactericidal Absorbent Dressing and Method of Fabrication Field of the Tnvenrinn This invention relates generally to absorbent dressings, and more particularly highly- absorbent synthetic polymer dressings having antimicrobial agents attached thereto. Bafinfrrmmd, of the Inventiflfi Bacterial growth in absorbent dressings for wounds, urinary incontinence diapers, and menstruation pads can lead to serious medical complications as well as social rfiffi g »irie< For example, bacterial growth in urinary incontinence diapers or menstruation pads usually produces strong, unpleasant odors that are socially unacceptable and can cause persons to alter their lifestyle. Conventional absorbent pads for urinary mconrinence and menstruation are not inherently bactericidal. Consequently, the only way to avoid growth of bacteria in the absorbent dressings is to change them at frequent intervals, even if the absorbent capacity of the pad has not beeα reached. In the area of wound dressings, bacterial contarnination of acute wounds and infection of chronic skin wounds are major clinical problems that can result in significant morbidity and, in severe cases, mortality. Conventionally, wound dressings have been designed to absorb wound fluids and yet provide a moist environment for promoting wound healing. However, such moist environments create a nutrient rich reservoir for bacterial growth in the dressing. Bacteria growing in the dressing can be shed back into the wound, increasing the risk of wound infection, or response to toxins, and producing strong, foul odors. In an effort to address these problems, antibiotics or chemical disinfectants are frequently applied topically to wounds prior to covering the wound with a dressing. Alternatively, topical agents are sometimes applied directly to the surface of the dressing. To control foul odors, some known dressings incorporate charcoal powder to absorb molecules generating the foul odor. For some applications, topical application of antibacterial agents is net d sirable. 5cr instance, tac'.ericidύi agents applied lopically to vcund irsM ff igs >.av έ a tendency to seep into the wound being created. Furthermore, many antimicrobial drugs, such as iodine, are cytotoxic and will retard wound healing if used repetitively or at high concentrations. A composition comprising a superabsorbent polymer having a monolayer (or near monolayer) of silane antimicrobial agent in a covalent bonding relationship with the base polymer is disclosed in U.S. Patent No. 5 ,045,322. The composition may be in the form of flakes, strips, powders, filaments, fibers or films, and may be applied to a substrate in the form of a coating. The aforementioned composition is less apt to enter a wound vis-a-vis conventional topical treatment systems. In that respect, the disclosed composition provides an improvement over conventional topical treatment systems. However, silanes contain siioxane bonds which can be cleaved by acids and bases produced by infection or bacterial growth. In turn, these reactions may weaken or destroy bonds between die silane antimicrobial agent and the underlying polymer. Consequently, antimicrobial agent may seep into a wound and retard wound healing. The need exists for an improved antimicrobial dressing composition having an antimicrobial agent which can be maintained securely attached to a superabsorbent polymer upon exposure to acids and bases produced by infection and bacterial growth. In addition to reducing the propensity for detachment of the antimicrobial agent, it would be desirable to provide a surface area enhanced dressing structure for increasing the effectiveness of the antimicrobial agent. Summary of the TπvftnriQn It is an object of the present invention to provide an inherently bactericidal superacscrbant dressing having an enhanced surface area. It is another object of the present invention to provide an inherently bactericidal superabsorbant dressing having an improved bactericidal attachment structure that resists degradation upon exposure to acids or bases produced, for instance, during bacterial growth. These and other objects are achieved by the inherently bactericidal polymer composition of the present invention. In the preferred embodiment, the composition comprises a polymer matrix having quaternary ammonium groups tethered to its surface through non- sϋoxane bonds. The surface area of the polymer matrix is enhanced, for instance, by electrostatically spinning a fiber-forming synthetic polymer to form a f ayed fiber or filament. Alternatively, the polymer solution can be wet- or dry-spun to create a roughened fiber surface by controlling the choice of solvent and the polymer solution temperature. Additional surface area enhancement is provided by tethering molecular chains of quaternary ammonium pendent groups to the surface of the polymer matrix. Tethering may be accomplished by known techniques such as grafting and selective adsorption. In an alternate embodiment of the invention, non-ionic bactericidal molecules are coupled to the surface of the polymer matrix, in lieu of ionically-charged molecules. Ionically-charged molecules are prone to being neutralized upon encountering oppositely- charged molecules. For instance, positively-charged quaternary ammonium groups may be neutralized by negatively-charged chloride ions present in physiological fluids. In instances were such neutralization is significant enough to reduce the bactericidal properties of the dressing below an acceptable level, non-ionic surface groups may be preferable. Detailed DftςgπptiQn of the refer^ F.mhodτττ nfs A novel antibacterial polymer composition is fabricated to have an enhanced surface area and superabsorbent capacity for biological fluids, including urine, blood, and wound In the preferred embodiment of the present invention, the composition includes a polymer matrix having quaternary ammonium compounds attached to the surface of the polymer matrix. The polymer matrix is comprised of a plurality of hydrophilic fibers or filaments which can be fabricated in any suitable manner. For example, suitable fibers or filaments can be fabricated by wet- or dry-spinning a fiber-forming synthetic polymer from a spinning solvent. The resulting polymer has superabsorbent capacity. Generally, polymers capable of absorbing from about thirty to sixty grams of water per gram of polymer are considered to be superabsorbent. Examples of superabsorbent polymers which can be fabricated in this manner include poiyacrylic acids, polyethylene oxides and polyvinyl alcohols. For example, methods for spinning polyethylene oxide using acetone solvent are well known. Significantly, the polymer matrix is fabricated to have an enhanced surface area. Enhancing the surface area of the polymer matrix results in improved absorption of biological fluids, and increases the availability of sites for attachment of the antimicrobial quaternary ammonium compounds. A corresponding increase in the quantity and density of antimicrobial sites, in turn, enhances the efficacy of the composition in killing organisms such as bacteria and viruses. It may occur to one skilled in the art of polymer science that a variety of methods are available for accomplishing surface area modification. Preferably, surface area enhancement is accomplished by a modified spinning or casting method. For instance, electrostatic spinning is a modified spinning technique which results in fraying of the fiber as it exits the spinere te. Alternatively, a polymer solution can be wet- or dry-spun to create a roughened fiber surface by controlling the solvent type and the polymer solution temperature. This technology is well known and has been applied, for example, in the manufacture of asymmetric membranes having roughened pores for dialysis. The size of the roughened pores is primarily controlled by the speed of precipitation which, in turn, is controlled by solvent interaction parameters, temperature, etc. The surface area of the polymer composition is further enhaπcsd by tethering chains of anάπύcrcbiai groups to the outer s rrace ci " the individual polymer fibers. Fraiex d biy, molecular chains of quaternary ammonium pendent groups are fabricated to have at least cπc end adapted for attachment to a fiber surface. For instance, suri ce grafting may be accomplished by creating surface free radicals as initiation sites from peroxide generation (ozone or microwave). Alternatively, surface attachment of an mterpenetrating network may be achieved using a monomer which swells the substrate polymer. The incorporation of tethered antimicrobial chains has the further benefit of enhancing the functionality of the composition. In particular, the tethered antimicrobial chains extend into the particular biological solution to bind to harmful bacterial and viral organisms. In contrast to known dressing compositions in which a monolayer (or near monolayer) of bactericidal compound is directly attached to a fiber surface, the chain structures of the present invention, which function like arms extending outwardly from the fiber surface, more effectively bind the antimicrobial sites to harmful organisms. Preferably, tethering is accomplished by grafting the antimicrobial chains directly to the matrix surface, or by selective adsorption of a copolymer to the matrix surface. Grafting techniques are well known in the art For example, quaternary- ammonium compound grafting using the monomer trirαemylanunonium ethyl met acrylate to graft polymerize to a modified polyethylene surface is described by Yahaioui (Master's Thesis, University of Florida, 1986). Yahioui describes a grafting technique in which a plasma discharge is used to create free radicals which initiate polymerization of appropriate monomers. Selective adsorption of appropriate block cσpσly ers can also be used. la contrast to known compositions in which an antimicrobial structure is achieved by covalently bonding silane groups to the surface of the base polymer, the present invention incorporates a chemical structure which is based on polymerization ( e., surface grafting) of monomers containing all carbon-carboα, carbon-oxygen and carboa-αitrogen main bonds, such as the dialkly, diallyl, quaternary ammonium compounds. Consequently, the composition of the present invention results in a structure which is less prone to reacting with acids and bases produced by bacterial growth. As previously mentioned, such reactions can degrade the attachment between the matrix and antimicrobial groups. In instances where the composition is ip ilad ' .o a wound dr≥ssiΛg. suc degradation ςcuiά rssuic in antimicrobial ≤genis detaching from the polymer matrix and entering a wound site. In some cases, this can have the deleterious effect of retarding wound healing. In an alternate embodiment of the present invention, anionic antibacteriάdal groups are immobilized on the surface of a supexabsorbant dressing to improve the antibactericid.il efficacy of the dressing. The positive charge associated with quaternary ammonium groups, for example, can be neutralized by negative ions, such as chloride ions present in physiological fluids such as urine and plasma. For applications where the degree of neutralization will significantly reduce the effectiveness of the antibactericidal agent, anionic surface groups can be substituted for quaternary ammonium groups. Examples of chemical compounds that can be used to produce immobilized anionic surface groups include Triton-100, Tween 20 and deoxychαlate. For instance, Triton- 100 contains a free hydroxyl group which can be derivatized into a good leaving group, such as tosyl or chloride, and subsequently reacted with a base-treated polymer, such as methyl cellulose, to yield a surface immobilized non-ionic surfac'ant Dimethyldiallyl ammonium chloride is one example of a suitable monomer which may be used with the present invention. This monomer, commonly referred to as B DAC or DADMAC, is used in the fabrication of commercial flocculating polymers. Modifications of trialkyl(p-vinylbenzyl) ammonium chloride or the p-trialkylaminoethyl styrene monomers are also suitable. One such example is trimethyl(p-vinyl benzyl) ammonium chloride; the methyl groups of this monomer can be replaced by other alkyl groups to impart desired properties. Alternatively, methacrylate-based monomers may be used; however, they may suffer from hydrαlytic instability under acidic and basic conditions in a fashion similar to the silane-based treatments of the prior art. Consequently, methacrylate-based monomers are not preferred. While the preferred embodiments of the invention have been illustrated and described, it will be clear that the invention is not so limited. Numerous modifications, changes, variations, substitutions and equivalents will occur to those skilled in the art without departing from the spirit and scope of the present invention as described in the claims.

Claims: We claim: 1. A dressing for absorbing biological fluids, comprising: a supertώ scr en' pciy r.sr masd:. a"Λr.g an arihaiiced s r ace arss.; zzd a plurality of antimicrobial compounds coupled by non-siloxane bonds to said polymer matrix. 2. A dressing as recited in claim 1, wherein said plurality of antimicrobial compounds comprise quaternary ammonium compounds* 3. A dressing as recited in claim 1, wherein said antimicrobial compounds comprise chain-like structures tethered at one end to said polymer matrix. 4. A dressing as recited in claim 1, wherein said plurality of antimicrobial compounds are non-ionic compounds. 5. A dressing as recited in claim 1, wherein said dressing comprises a sanitary pad. 6. A dressing as recited in claim 1, wherein said dressing comprises a tampon. 7. A dressing as recited in claim 1, wherein said dressing comprises a bandage. 8. A method for fabricating an intrinsically antimicrobial absorbent dressing, comprising the steps of: forming a superabsorbent synthetic polymer matrix having an enhanced surface area; and attaching a plurality of antimicrobial compounds to the enhanced surface area of said polymer matrix.

Inventors: BATICH CHRISTOPHER D, LERNER DAVID, MAST BRUCE A, OLDERMAN GERALD M, SCHULTZ GREGORY S, BATICH, CHRISTOPHER, D., LERNER, DAVID, MAST, BRUCE, A., OLDERMAN, GERALD, M., SCHULTZ, GREGORY, S.
Applicants: BATICH CHRISTOPHER D, LERNER DAVID, MAST BRUCE A, OLDERMAN GERALD M, QUICK MED TECHNOLOGIES INC, SCHULTZ GREGORY S, UNIV FLORIDA, BATICH, CHRISTOPHER, D., LERNER, DAVID, MAST, BRUCE, A., OLDERMAN, GERALD, M., QUICK-MED TECHNOLOGIES, INC., SCHULTZ, GREGORY, S., UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.
Application year: 1999
Main classification: A61F13
IPC codes: A61F5, A61F13, A61L2