THERAPEUTIC ANTIANGIOGENIC ENDOSTATIN COMPOSITIONS

Abstract: Endostatin compositions capable of inhibiting endothelial cell proliferation, inhibiting angiogenesis and causing tumor regression are described. Specifically, amino acid sequences of endostatin proteins and nucleic acid sequences coding for endostatin proteins are provided.

Description: THERAPEUTIC ANTIANGIOGENIC ENDOSTATIN COMPOSITIONS Cross Reference to Prior Related Cases This application claims priority to provisional application 60/106,343 filed October 30, 1998 The above-referenced application is incorporated herein in its entirety This invention may have been made in part by funds from NIH grants RO1- CA64481 and PO1-CA45548. The U.S. government may have certain rights in this invention. Technical Field This application relates to a novel inhibitor of angiogenesis useful for treating angiogenesis-related diseases, such as angiogenesis-dependent cancer. The invention further relates to a novel composition and method for curing angiogenesis-dependent cancer. In addition, the present invention relates to diagnostic assays and kits for endostatin measurement, to histochemical kits for localization of endostatin, to molecular probes to monitor endostatin biosynthesis, to antibodies that are specific for the endostatin, to the development of peptide agonists and antagonists to the endostatin receptor, and to cytotoxic agents linked to endostatin peptides. Background of the Invention Several lines of direct evidence now suggest that angiogenesis is essential for the growth and persistence of solid tumors and their metastases (Folkman, 1989; Hoπ et al., 1991, Kim et al., 1993; Millauer et al., 1994). To stimulate angiogenesis, tumors up- regulate their production of a variety of angiogenic factors, including the fibroblast growth factors (FGF and BFGF) (Kandel et al., 1991) and vascular endothelial cell growth factor/vascular permeability factor (VEGF/VPF). However, many malignant tumors also generate inhibitors of angiogenesis, including angiostatin and thrombospondin (Chen et al , 1995; Good et al , 1990; O'Reilly et al., 1994) It is postulated that the angiogenic phenotype is the result of a net balance between these positive and negative regulators of neovasculaπzation (Good et al., 1990; O'Reilly et al., 1994; Parangi et al , 1996, Rastinejad et al., 1989) Several other endogenous inhibitors of angiogenesis have been identified, although not all are associated with the presence of a tumor. These include, platelet factor 4 (Gupta et al , 1995; Maione et al., 1990), interferon-alpha, mterferon-inducible protein 10 (Angio llo et al , 1995; Stneter et al., 1995), which is induced by ιnterleukιn-12 and/or interferon-gamma (Voest et al , 1995), gro-beta (Cao et al., 1995), and the 16 kDa N-terminal fragment of prolactin (Clapp et al., 1993). The only known angiogenesis inhibitor which specifically inhibits endothelial cell proliferation is angiostatin (O'Reilly et al 1994). Angiostatin is an approximately 38 kiloDalton (kDa) specific inhibitor of endothelial cell proliferation. Angiostatin is an internal fragment of plasmmogen containing at least three of the five kπngles of plasmmogen. Angiostatin has been shown to reduce tumor weight and to inhibit metastasis in certain tumor models. (O'Reilly et al., 1994). As it is used hereinafter, the term "angiostatin" refers to angiostatin as descπbed above; peptide fragments of angiostatin that have endothelial cell proliferation inhibiting activity, and analogs of angiostatin that have substantial sequence homology (as defined herein) to the amino acid sequence of angiostatin, which have endothelial cell proliferation inhibiting activity. Summary of the Invention The present invention relates to a novel protein inhibitor, and method for its use. The protein is a potent and specific inhibitor of endothelial proliferation and angiogenesis. Systemic therapy with the inhibitor causes a nearly complete suppression of tumor-mduced angiogenesis, and it exhibits strong anti-tumor activity. The inhibitory protein has a molecular weight of approximately 18,000 to approximately 20,000 Daltons (18 to 20 kDa) and is capable of inhibiting endothelial cell proliferation in cultured endothelial cells. The protein can be further characterized by its preferred N-terminal ammo acid sequence, the first twenty (20) of which are as follows: His Thr His Gin Asp Phe Gin Pro Val Leu 1 2 3 4 5 6 7 8 9 10 His Leu Val Ala Leu Asn Thr Pro Leu Ser 11 12 13 14 15 16 17 18 19 20 (SEQ ID NO:l) A preferred endothelial cell proliferation inhibitor of the invention is a protein having the above-descπbed charactenstics, and which can be isolated and punfied from the muπne hemangioendothehoma cell line EOMA This inhibitory protein has been named endostatin. The present invention provides methods and compositions for treating diseases and processes mediated by undesired and uncontrolled angiogenesis by administenng to a human or animal with the undesired angiogenesis a composition comprising a substantially purified endostatin or endostatin derivative in a dosage sufficient to inhibit angiogenesis. The present invention is particularly useful for treating or for repressing the growth of tumors. Administration of endostatin to a human or animal with prevasculaπzed metastasized tumors prevents the growth or expansion of those tumors. The present invention also includes diagnostic methods and kits for detection and measurement of endostatin in biological fluids and tissues, and for localization of endostatin in tissues. The diagnostic method and kit can be in any configuration well known to those of ordinary skill in the art. The present invention also includes antibodies specific for the endostatin and antibodies that inhibit the binding of antibodies specific for the endostatin. These antibodies can be polyclonal antibodies or monoclonal antibodies. The antibodies specific for endostatin can be used in diagnostic kits to detect the presence and quantity of endostatin which is diagnostic or prognostic for the occurrence or recurrence of cancer or other diseases mediated by angiogenesis. Antibodies specific for endostatin may also be administered to a human or animal to passively immunize the human or animal against endostatin, thereby reducing angiogenic inhibition. The present invention also includes diagnostic methods and kits for detecting the presence and quantity of antibodies that bind endostatin in body fluids. The diagnostic method and kit can be in any configuration well known to those of ordinary skill in the art. The present invention also includes endostatin peptide fragments that can be labeled isotopically or with other molecules or proteins for use m the detection and visualization of endostatin binding sites with state of the art techniques, including, but not limited to, positron emission tomography, autoradiography, flow cytometry, radioreceptor binding assays, and immunohistochemistry. These endostatin peptides also act as agonists and antagonists at the endostatin receptor, thereby enhancing or blocking the biological activity of endostatin. Such peptides are used in the isolation of the endostatin receptor. The present invention also includes endostatin, endostatin fragments, endostatin antisera, or endostatin receptor agonists and antagonists linked to cytotoxic agents for therapeutic and research applications. The present invention includes molecular probes for the πbonucleic acid and deoxyπbonucleic acid involved in transcription and translation of endostatin. These molecular probes provide means to detect and measure endostatin biosynthesis in tissues and cells A surprising discovery is that various forms of recombinant endostatin protein can serve as sustained release anti-angiogenesis compounds when administered to a tumor-bearing animal. A preferred form of the sustained release compound is un- refolded recombinantly produced endostatin Additionally, the present invention encompasses nucleic acid sequences comprising corresponding nucleotide codons that code for the above disclosed ammo acid sequence and for endostatin and endothelial cell proliferation inhibiting peptide fragments thereof . The present invention also relates to methods of using the endostatin protein and peptide fragments, corresponding nucleic acid sequences, and antibodies that bind specifically to the inhibitor and its peptides, to diagnose endothelial cell-related diseases and disorders The invention further encompasses a method for identifying receptors specific for endostatin, and the receptor molecules identified and isolated thereby. The invention also relates to a method for identifying novel enzymes capable of releasing endostatin from collagen type XVIII, and other molecules containing an endostatin amino acid sequence, and peptides thereof. Such endostatin producing enzymes are also an aspect of the invention. An important medical method is a new form of birth control, wherein an effective amount of endostatin is administered to a female such that uterine endometπal vasculaπzation is inhibited and embryo implantation cannot occur, or be sustained. A particularly important aspect of the present invention is the discovery of a novel and effective method for treating angiogenesis-related diseases, particularly angiogenesis-dependent cancer, in patients, and for curing angiogenesis-dependent cancer in patients. The method unexpectedly provides the medically important result of inhibition of tumor growth and reduction of tumor mass. The method relates to the co- admmistration of the endostatin of the present invention and another anti-angiogenesis compound, preferably angiostatin. Accordingly, the present invention also includes formulations containing endostatin, and optionally angiostatin, which are effective for treating or cuπng angiogenesis-dependent cancers. Accordingly, it is an object of the present invention to provide a composition comprising an endostatin protein It is another object of the present invention to provide a method of treating diseases and processes that are mediated by angiogenesis. It is yet another object of the present invention to provide a diagnostic or prognostic method and kit for detecting the presence and amount of endostatin in a body fluid or tissue It is yet another object of the present invention to provide a method and composition for treating diseases and processes that are mediated by angiogenesis including, but not limited to, hemangioma, solid tumors, leukemia, metastasis, telangiectasia psoriasis scleroderma, pyogenic granuloma, myocardial angiogenesis, plaque neovasculaπzation. coronary collaterals, cerebral collaterals, artenovenous malformations, lschemic limb angiogenesis, corneal diseases, rubeosis, neovascular glaucoma, diabetic retmopathy, retrolental fibroplasia, arthritis, diabetic neovasculaπzation, macular degeneration, wound healing, peptic ulcer, fractures, keloids, vasculogenesis, hematopoiesis. ovulation, menstruation, and placentation. It is another object of the present invention to provide a composition for treating or repressing the growth of a cancer. It is an object of present invention to provide a method for detecting and quantifying the presence of an antibody specific for an endostatin m a body fluid. Still another object of the present invention is to provide a composition consisting of antibodies to endostatin that are selective for specific regions of the endostatin molecule It is another object of the present invention to provide a method for the detection or prognosis of cancer. It is another object of the present invention to provide a composition for use in visualizing and quantitating sites of endostatin binding in vivo and in vitro. It is yet another object of the present invention to provide a composition for use in detection and quantification of endostatin biosynthesis. It is yet another object of the present invention to provide a therapy for cancer that has minimal side effects Still another object of the present invention is to provide a composition comprising endostatin or an endostatin peptide linked to a cytotoxic agent for treating or repressing the growth of a cancer. These and other objects, features and advantages of the present invention will become apparent after a review of the following detailed description of the disclosed embodiments and the appended claims. Brief Description of the Figures Figure 1 : Inhibition of Capillary Endothelial Cell Proliferation by Conditioned Media from EOMA Cells. Conditioned media collected from confluent EOMA cells or base media was applied to bovine capillary endothelial cells with 1 ng/ml bFGF in a 72 hour proliferation assay. Endothelial cell proliferation was inhibited by the EOMA conditioned media. Each bar represents the mean ± SEM. Figure 2: Purification of an Inhibitor of Endothelial Proliferation from EOMA Conditioned Media. Conditioned media collected from confluent EOMA cells was fractionated on a hepann sepharose column. Endothelial proliferation inhibiting activity eluted at approximately 0.8M NaCl. Figure 3: Purification of an Inhibitor of Endothelial Proliferation by Gel Filtration. Punfied inhibitor from hepann sepharose column chromatography was applied to a gel filtration column and eluted as a single peak. Figure 4: Purification of Inhibitor of Endothelial Cell Proliferation by Reversed Phase Column Chromatography. Inhibitor purified by hepann sepharose and gel filtration chromatography was applied to a reverse phase column. The inhibitor eluted as a single band from the column at approximately 45% of the acetonitnle. Figure 5: N -terminal Amino Acid Sequence of An Inhibitor of Endothelial Cell Proliferation. The N-terminal sequence of the purified inhibitor of endothelial cell proliferation is shown in relation to a schematic diagram of collagen type 18. The N-terminal sequence revealed identity of the inhibitor to an approximately 20 kDa C-terminal fragment (shown in solid shading) for collagen type XVIII. The open boxes represent the collagenase domains of collagen type XVIII. Figure 6: Treatment of Lewis Lung Carcinoma With Recombinant Mouse Endostatin Inhibitor. Recombinant inhibitor produced in E. coli was administered to mice seeded with Lewis lung carcinoma that had achieved a tumor volume of approximately 150 mm^. The inhibitor was administered at 20 mg/kg/day. Tumor mass regressed to non- detectable levels after approximately 12 days of treatment. Figure 7A-C: Systemic Therapy with Recombinant Endostatin Regresses Lewis Lung Carcinoma Primary Tumors. Figure 7A. Mice were implanted subcutaneously on the dorsum with Lewis lung carcinoma cells. Systemic therapy with recombinant mouse endostatin (20 mg/kg/day) was begun when tumors were approximately 200 mm^ (1% of body weight). Tumors in the mice treated with endostatin inhibitor rapidly regressed and were inhibited by >99% relative to saline-treated controls. Each point represents mean + SEM for 5 mice. The experiment was repeated with comparable results. Figure 7B Representative treated and untreated tumor-bearing mice after 1 1 days of systemic therapy with endostatin Saline-treated mice (right) had rapidly growing red tumors with ulcerated surfaces Endostatin treated mice (left) had small pale residual tumors (arrow) Figure 7C Residual disease in endostatin treated mice. Three of the five endostatin treated mice were sacrificed after 16 days of therapy. Autopsy revealed small white residual tumors at the site of the onginal pnmary implantation (arrows). Figure 8: Treatment of Murine T241 Fibrosarcoma with Recombinant Mouse Endostatin from E. coli Mice were seated with T241 Fibrosarcoma cells. Control mice were treated with saline. Experimental mice were treated with 20 mg/kg/day of recombinant mouse Endostatin directed from E. coli. Figure 9: Treatment of Murine B16F10 Melanoma with Recombinant Mouse Endostatin Mice were seated with Murine B 16F10 melanoma cells. Control animals were treated with saline. Experimental animals were treated with 20 mg/kg/day of recombinant mouse Endostatin direct from E. coli Figure 10. Treatment of EOMA Hemangioendothelwma with Recombinant Mouse Endostatin from E. coli Mice were seated with EOMA hemangioendothehoma cells. Control animals were treated with saline. Experimental animals were treated with 20 mg/kg/day of Recombinant Mouse Endostatin direct from E. coli. Figure 1 1 : Treatment of Lewis Lung Carcinoma with Recombinant Mouse or Human Endostatin direct from E. coli. Mice were seated with Lewis Lung Carcinoma cells. Control animals were treated with saline. Experimental animals were treated with Recombinant Endostatin derived from the mouse sequence or Recombinant Endostatin direct from the human sequence, wherein both Endostatins were produced recombmantly in the E. coli. Mouse Endostatin was administered at either 20 mg/kg/day or 2.5 mg/kg/day, and Human Endostatin was administered at 20 mg/kg/day Figure 12A-C: Endostatin Results in an Inhibition of Angiogenesis and an Increase in Apoptosis of Lewis Lung Carcinoma Primary Tumors. 1 Histological sections of tumors from saline versus endostatin treated mice implanted with Lewis lung carcinomas were analyzed for proliferation (PCNA), apoptosis (TUNEL), and angiogenesis (vWF) There was no significant difference in the proliferative index of tumor cells (Figure 12A) in treated versus untreated tumors. In contrast, the apoptotic index of the tumor cells (Figure 12B) increased 8-fold (p < 0.001) in the endostatin treated mice. Vessel density (Figure 12C) was determined by counting the number of capillary blood vessels per high-power field (HPF) in sections stained with antibodies against vWF. Angiogenesis was almost completely suppressed in the residual microscopic tumors of the endostatin treated mice (p < 0.001). Figure 13 Cycle Dormancy Therapy of Lewis Lung Carcinoma with Recombinant Mouse Endostatin From E. Coli. Mice were implanted subcutaneously on the dorsum with Lewis lung carcinoma cells. Systemic therapy with recombinant mouse inhibitor (endostatin), administered at a dose of 20 mg/kg/day, was begun when tumors were approximately 200 mm 3 (1% of body weight). Tumors in the mice treated with the endostatin inhibitor rapidly regressed to essentially non-detectable levels after approximately 15 days of therapy. When treatment was terminated the tumor volume increased rapidly and was subsequently treatable to the same non-detectable levels by re- initiation of treatment. The peaks and valleys in the figure show the cycling effect of inhibition with endostatin. Figure 14. Combination Therapy with Recombinant Mouse Angiostatin and Endostatin from E. Coli. Mice were implanted subcutaneously on the dorsum with Lewis lung carcinoma cells. Systemic therapy with a combination of recombinant mouse endostatin (20 mg/kg/day) and recombinant mouse angiostatin (20 mg/kg/day) was begun when tumors were approximately 300 τc? . Tumors in the mice treated with the combination therapy rapidly regressed to essentially non-detectable levels in about 15 days. Importantly, the regressed tumors remained dormant and did not increase in size or mass after treatment was stopped. This is an unexpected result of substantial medical significance. Detailed Description of the Invention Applicants have discovered a new class of protein molecules that have the ability to inhibit endothelial proliferation when added to proliferating endothelial cells in vitro. Accordingly, these protein molecules have been functionally defined as endostatins, however, it is to be understood that this functional definition is no way limits the bioactivity of endostatins to inhibition of endothelial cell growth in vitro or in vivo. Many other functions of endostatins are likely The term "endostatin" refers to a protein that is preferably 18 kDa to 20 kDa in size as determined by non-reduced and reduced gel electrophoresis, respectively. The term endostatin also includes precursor forms of the 18 kDa to 20 kDa protein The term endostatin also includes fragments of the 18 kDa to 20 kDa protein and modified proteins and peptides that have a substantially similar amino acid sequence, and which are capable inhibiting proliferation of endothelial cells For example, silent substitutions of ammo acids, wherein the replacement of an amino acid with a structurally or chemically similar amino acid does not significantly alter the structure, conformation or activity of the protein, is well known in the art. Such silent substitutions are intended to fall within the scope of the appended claims. It will be appreciated that the term "endostatin" includes shortened proteins or peptides wherein one or more ammo acid is removed from either or both ends of endostatin, or from an internal region of the protein, yet the resulting molecule retains endothelial proliferation inhibiting activity The term "endostatin" also includes lengthened proteins or peptides wherein one or more ammo acid is added to either or both ends of endostatin, or to an internal location in the protein, yet the resulting molecule retains endothelial proliferation inhibiting activity. For example, molecules 125 with tyrosine added in the first position can be labeled with iodine for use in assays. Labeling with other radioisotopes may be useful in providing a molecular tool for destroying the target cell containing endostatin receptors. Other labeling with molecules such as πcin may provide a mechanism for destroying cells with endostatin receptors. "Substantial sequence homology" means at least approximately 70% homology between ammo acid residue sequence in the endostatin analog sequence and that of endostatin, preferably at least approximately 80% homology, more preferably at least approximately 90% homology Also included in the definition of the term endostatin are modifications of the endostatin protein, its subunits and peptide fragments. Such modifications include substitutions of naturally occurnng ammo acids at specific sites with other molecules, including but not limited to naturally and non-naturally occurring amino acids. Such substitutions may modify the bioactivity of endostatin and produce biological or pharmacological agonists or antagonists. The term endostatin also includes an N- terminal fragment of endostatin consisting of the sequence of the first 20 N-termmal amino acids which is shown in SEQ ID NOJ and is shown in Table 1. This sequence of the first 20 N-terminal amino acids corresponds to a C-termmal fragment of newly identified collagen type XVIII. Table 1 shows the correspondence of 3 letter and 1 letter ammo acid designations. TABLE 1 Amino Acid Residue Abbreviation 1 HIS H 2 THR T 3 HIS H 4 GLN Q 5 ASP D 6 PHE F 7 GLN Q 8 PRO P 9 VAL V 10 LEU L 11 HIS H 12 LEU L 13 VAL V 14 ALA A 15 LEU L 16 ASN N 17 THR T 18 PRO P 19 LEU L 20 SER S The N-termmal ammo acid sequence of endostatin corresponds to an internal 20 amino acid peptide fragment found in mouse collagen alpha 1 type XVIII starting at ammo acid 1105 and ending at amino acid 1124. The N-termmal amino acid sequence of the inhibitor also corresponds to an internal 20 amino acid peptide fragment found in human collagen alpha 1 type XVIII starting at amino acid 1132 and ending at amino acid 1151. Endostatin can be isolated from murine hemangioendothehoma EOMA. Endostatin may be produced from recombinant sources, from genetically altered cells implanted into animals, from tumors, and from cell cultures as well as other sources. It is anticipated that endostatin is made in cells of the nervous system. Endostatin can be isolated from body fluids including, but not limited to, serum, urine and ascites, or synthesized by chemical or biological methods (e.g. cell culture, recombinant gene expression, peptide synthesis-and in vitro enzymatic catalysis of precursor molecules to yield active endostatin). Recombinant techniques include gene amplification from DNA sources using the polymerase chain reaction (PCR), and gene amplification from RNA sources using reverse transcnptase/PCR. Endostatin specifically and reversibly inhibits endothelial cell proliferation. The inhibitor protein molecules of the invention are useful as a birth control drug, and for treating angiogenesis-related diseases, particularly angiogenesis-dependent cancers and tumors. The protein molecules are also useful for cunng angiogenesis-dependent cancers and tumors. The unexpected and surprising ability of these novel compounds to treat and cure angiogenesis-dependent cancers and tumors answers a long felt unfulfilled need in the medical arts, and provides an important benefit to mankind. Important terms that are used herein are defined as follows "Cancer" means angiogenesis-dependent cancers and tumors, 1 e tumors that require for their growth (expansion in volume and/or mass) an increase in the number and density of the blood vessels supplying them with blood. "Regression" refers to the reduction of tumor mass and size. The endothelial proliferation inhibiting proteins of the present invention can be made by automated protein synthesis methodologies well known to one skilled in the art. Alternatively, endothelial proliferation inhibiting proteins, or endostatins, of the present invention may be isolated from larger known proteins, such as human alpha 1 type XVIII collagen and mouse alpha 1 type XVIII collagen, proteins that share a common or similar N-termmal amino acid sequence. Examples of other potential endostatin source materials having similar N-terminal amino acid sequences include Bos taunts pregastπc esterase, human alpha 1 type 15 collagen, NAD-dependent formate dehydrogenase (EC 1.2.1.2) denved from Pseudomonas sp.. si 1459 hexon protein of bovine adenovirus type 3, CELF21D12 2 F21dl2.3 Caenorhabditis elegans gene product, VAL1 TGMV AL1 protein derived from tomato golden mosaic virus, s01730 hexon protein derived from human adenovirus 12, Saccharomyces cerevisiae. For example, peptides closely related to endostatin may be derived from BOVMPE 1 pregastnc esterase (BOS TAURUS) gene sequence corresponding to amino acids 502 to 521, and collagen alpha 1 type 15 from humans beginning at amino acid 316 ending at 335. Proteins and peptides derived from these and other sources, including manual or automated protein synthesis, may be quickly and easily tested for endothelial proliferation inhibiting activity using a biological activity assay such as the bovine capillary endothelial cell proliferation assay. Other bioassays for inhibiting activity include the chick CAM assay, the mouse corneal assay, and the effect of administering isolated or synthesized proteins on implanted tumors. The chick CAM assay is descπbed by O'Reilly, et al. in "Angiogenic Regulation of Metastatic Growth" Cell, vol. 79 (2), October 21, 1994, pp. 315-328, which is hereby incorporated by reference in its entirety. Briefly, 3 day old chicken embryos with intact yolks are separated from the egg and placed in a petπ dish. After 3 days of incubation, a methylcellulose disc containing the protein to be tested is applied to the CAM of individual embryos. After 48 hours of incubation, the embryos and CAMs are observed to determine whether endothelial growth has been inhibited. The mouse corneal assay involves implanting a growth factor-containing pellet, along with another pellet containing the suspected endothelial growth inhibitor, in the cornea of a mouse and observing the pattern of capillaries that are elaborated in the cornea. Applicants' invention also encompasses nucleic acid sequences that correspond to and code for the endothelial proliferation-inhibiting protein molecules of the invention, and to monoclonal and polyclonal antibodies that bind specifically to such protein molecules. The biologically active protein molecules, nucleic acid sequences conesponding to the proteins, and antibodies that bind specifically to the proteins of the present invention are useful for modulating endothelial processes in vivo, and for diagnosing and treating endothelial cell-related diseases, for example by gene therapy. Nucleic acid sequences that correspond to, and code for, endostatin and endostatin analogs can be prepared based upon the knowledge of the amino acid sequence, and the art recognized conespondence between codons (sequences of three nucleic acid bases), and amino acids: Because of the degeneracy of the genetic code, wherein the third base in a codon may vary yet still code for the same amino acid, many different possible coding nucleic acid sequences are derivable for any particular protein or peptide fragment. Nucleic acid sequences are synthesized using automated systems well known in the art. Either the entire sequence may be synthesized or a series of smaller oligonucleotides are made and subsequently ligated together to yield the full length sequence. Alternatively, the nucleic acid sequence may be derived from a gene bank using oligonucleotides probes designed based on the N-terminal amino acid sequence and well known techniques for cloning genetic material. The present invention also includes the detection of endostatin in body fluids and tissues for the purpose of diagnosis or prognosis of angiogenesis-mediated diseases such as cancer. The present invention also includes the detection of endostatin binding sites and receptors in cells and tissues. The present invention also includes methods of treating or preventing angiogenic diseases and processes including, but not limited to, arthritis and tumors by stimulating the production of endostatin, and/or by administering substantially purified endostatin, or endostatin agonists or antagonists, and/or endostatin antisera or antisera directed against endostatin antisera to a patient. Additional treatment methods include administration of endostatin, endostatin fragments, endostatin antisera, or endostatin receptor agonists and antagonists linked to cytotoxic agents. It is to be understood that the endostatin can be animal or human in origin. Endostatin can also be produced synthetically by chemical reaction or by recombinant techniques in conjunction with expression systems. Endostatin can also be produced by enzymatically cleaving different molecules, including endostatin precursors, containing sequence homology or identity with segments of endostatin to generate peptides having anti-angiogenesis activity. Passive antibody therapy using antibodies that specifically bind endostatin can be employed to modulate endothelial-dependent processes such as reproduction, development, and wound healing and tissue repair. In addition, antisera directed to the Fab regions of endostatin antibodies can be administered to block the ability of endogenous endostatin antisera to bind endostatin. Antibodies specific for endostatin and endostatin analogs are made according to techniques and protocols well known in the art. The antibodies may be either polyclonal or monoclonal The antibodies are utilized in well know immunoassay formats, such as competitive and non-competitive immunoassays. including ELISA. sandwich immunoassays and ladioimmunoassays (RIAs), to determine the presence or absence ot the endothelial proliferation inhibitors of the present invention in body fluids. Examples of body fluids include but are not limited to blood, serum, pentoneal fluid, pleural fluid, cerebrospmal fluid, uterine fluid, saliva, and mucus. The proteins, nucleic acid sequences and antibodies of the present invention are useful for diagnosing and treating endothelial cell-related diseases and disorders. A particularly important endothelial cell process is angiogenesis, the formation of blood vessels. Angiogenesis-related diseases may be diagnosed and treated using the endothelial cell proliferation inhibiting proteins of the present invention. Angiogenesis- related diseases include, but are not limited to, angiogenesis-dependent cancer, including, for example, solid tumors, blood born tumors such as leukemias, and tumor metastases; benign tumors, for example hemangiomas. acoustic neuromas, neurofibromas, trachomas, and pyogenic granulomas; rheumatoid arthritis; psoriasis; ocular angiogenic diseases, for example, diabetic ret opathy, retmopathy of prematurity, macular degeneration, corneal graft rejection, neovascular glaucoma, retrolental fibroplasia, rubeosis; Osier- Webber Syndrome; myocardial angiogenesis; plaque neovasculaπzation; telangiectasia. hemophiliac joints; angiofibroma; and wound granulation. The endothelial cell proliferation inhibiting proteins of the present invention are useful in the treatment of disease of excessive or abnormal stimulation of endothelial cells. These diseases include, but are not limited to, intestinal adhesions, atherosclerosis, scleroderma, and hypertrophic scars, i.e., keloids. They are also useful in the treatment of diseases that have angiogenesis as a pathologic consequence such as cat scratch disease (Rochele minalia quintosa) and ulcers (Helobacter pylori) The endothelial cell proliferation inhibiting proteins can be used as a birth control agent by reducing or preventing uterine vasculaπzation required for embryo implantation. Thus, the present invention provides an effective birth control method when an amount of the inhibitory protein sufficient to prevent embryo implantation is administered to a female. In one aspect of the birth control method, an amount of the inhibiting protein sufficient to block embryo implantation is administered before or after intercourse and fertilization have occ red, thus providing an effective method of birth control, possibly a "morning after" method. While not wanting to be bound by this statement, it is believed that inhibition of vasculaπzation of the uterine endometπum interferes with implantation of the blastocyst Similar inhibition of vasculaπzation of the mucosa of the uterine tube interferes with implantation of the blastocyst, preventing occunence of a tubal pregnancy Administration methods may include, but are not limited to, pills, injections (intravenous, subcutaneous, intramuscular), suppositories, vaginal sponges, vaginal tampons, and mtrauteπne devices. It is also believed that endostatin administration will interfere with normal enhanced vascularization of the placenta, and also with the development of vessels within a successfully implanted blastocyst and developing embryo and fetus. Conversely, blockade of endostatin receptors with endostatin analogs that act as receptor antagonists may promote endothehahzation and vascularization. Such effects may be desirable in situations of inadequate vasculaπzation of the uterine endometπum and associated infertility, wound repair, healing of cuts and incisions, treatment of vascular problems in diabetics, especially retinal and peripheral vessels, promotion of vascularization in transplanted tissue including muscle and skin, promotion of vascularization of cardiac muscle especially following transplantation of a heart or heart tissue and after bypass surgery, promotion of vasculaπzation of solid and relatively avascular tumors for enhanced cytotoxin delivery, and enhancement of blood flow to the nervous system, including but not limited to the cerebral cortex and spinal cord. A surprising discovery is that un-refolded and non-soluble recombinant endostatin is also a potent anti-angiogenesis compound which serves as a sustained release depot when administered to a patient. The present invention also relates to methods of using endostatin and endothelial cell proliferation inhibiting peptide fragments of endostatin, nucleic acid sequences corresponding to endostatin and active peptide fragments thereof, and antibodies that bind specifically to endostatin and its peptides, to diagnose endothelial cell-related diseases and disorders. The invention further encompasses a method for identifying endostatm-specific receptors, and the receptor molecules identified and isolated thereby The present invention also provides a method for quantitation of endostatin receptors A particularly important aspect of the present invention is the discovery of a novel and effective method for treating and cuπng angiogenesis-dependent cancer in patients. It was unexpectedly found that the co-admmistration of endostatin and angiostatin in an amount sufficient to inhibit tumor growth and cause sustainable regression of tumor mass to microscopic size cures angiogenesis-dependent cancer. Accordingly, the present invention also includes formulations effective for treating or cuπng angiogenesis-dependent cancers and tumors. More particularly, recombinant mouse endostatin, from insect cells or E. coli, potently inhibits angiogenesis and the growth of metastases and primary tumors. In a novel method of sustained release, the E. coh-denved recombinant endostatin was administered as an un-refolded suspension in an amount sufficient to inhibit angiogenesis, thereby inhibiting tumor growth Tumor mass was reduced when recombinant endostatin was administered an amount sufficient to cause regression of the tumor Piimary tumors of 1-2% of body weight regressed by greater than 150-fold to become microscopic dormant lesions when treated by endostatin Immunohistochemical analysis of the dormant tumors revealed blocked angiogenesis accompanied by high proliferation of the tumor cells balanced by a high rate of tumor cell apoptosis There was no evidence of toxicity in any of the mice treated with endostatin. It is contemplated as part of the present invention that endostatin can be isolated from a body fluid such as blood or uπne of patients or the endostatin can be produced by recombinant DNA methods or synthetic peptide chemical methods that are well known to those of ordinary skill in the art Protein purification methods are well known in the art and a specific example of a method for purifying endostatin, and assaying for inhibitor activity is provided in the examples below Isolation of human endogenous endostatin is accomplished using similar techniques One example of a method of producing endostatin using recombinant DNA techniques entails the steps of (1) identifying and purifying an endostatin as discussed above, and as more fully described below, (2) determining the N-terminal amino acid sequence of the purified inhibitor, (3) synthetically generating a DNA ohgonucleotide probe that conesponds to the N-terminal amino acid sequence, (4) generating a DNA gene bank from human or other mammalian DNA, (5) probing the gene bank with the DNA ohgonucleotide probe, (6) selecting clones that hybπdize to the ohgonucleotide, (7) isolating the inhibitor gene from the clone, (8) inserting the gene into an appropriate vector such as an expression vector, (9) inserting the gene-containing vector into a microorganism or other expression system capable of expressing the inhibitor gene, and (10) isolating the recombinantly produced inhibitor. The above techniques are more fully described in laboratory manuals such as "Molecular Cloning. A Laboratory Manual" Second Edition by Sambrook et al., Cold Spπng Harbor Press, 1989 The gene for endostatin may also be isolated from cells or tissue (such as tumor cells) that express high levels of endostatin by (1) isolating messenger RNA from the tissue, (2) using reverse transcπptase to generate the conesponding DNA sequence and then (3) using PCR with the appropriate primers to amplify the DNA sequence coding for the active endostatin amino acid sequence Yet another method of producing endostatin, or biologically active fragments thereof, is by peptide synthesis Once a biologically active fragment of an endostatin is found using the assay system described more fully below, it can be sequenced, for example by automated peptide sequencing methods Alternatively, once the gene or DNA sequence which codes for endostatin is isolated, for example by the methods described above, the DNA sequence can be determined, which in turn provides information regarding the amino acid sequence. Thus, if the biologically active fragment is generated by specific methods, such as tryptic digests, or if the fragment is N-terminal sequenced, the remaining ammo acid sequence can be determined from the conesponding DNA sequence. Once the amino acid sequence of the peptide is known, for example the N- termmal 20 amino acids, the fragment can be synthesized by techniques well known in the art. as exemplified by "Solid Phase Peptide Synthesis A Practical Approach" E Atherton and R C. Sheppard. IRL Press, Oxford England Similarly, multiple fragments can be synthesized which are subsequently linked together to form larger fragments. These synthetic peptide fragments can also be made with amino acid substitutions at specific locations in order to test for agonistic and antagonistic activity in vitro and in vivo Peptide fragments that possess high affinity binding to tissues can be used to isolate the endostatin receptor on affinity columns Isolation and purification of the endostatin receptor is a fundamental step towards elucidating the mechanism of action of endostatin. This isolation facilitates development of drugs to modulate the activity of the endostatin receptor, the final pathway to biological activity. Isolation of the receptor enables the construction of nucleotide probes to monitor the location and synthesis of the receptor, using m situ and solution hybπdization technology. Endostatin is effective in treating diseases or processes that are mediated by, or involve, angiogenesis. The present invention includes the method of treating an angiogenesis mediated disease with an effective amount of endostatin or endostatin agonists and antagonists. The angiogenesis mediated diseases include, but are not limited to, solid tumors; blood born tumors such as leukemias; tumor metastasis: benign tumors, for example hemangiomas, acoustic neuromas, neurofibromas, trachomas, and pyogenic granulomas; rheumatoid arthritis; psoriasis; ocular angiogenic diseases, for example, diabetic retmopathy, retmopathy of prematuπty, macular degeneration, corneal graft rejection, neovascular glaucoma, retrolental fibroplasia, rubeosis; Osier- Webber Syndrome; myocardial angiogenesis; plaque neovasculaπzation; telangiectasia; hemophiliac joints; angiofibroma; and wound granulation. Endostatin is useful in the treatment of disease of excessive or abnormal stimulation of endothelial cells These diseases include, but are not limited to, intestinal adhesions, atherosclerosis, scleroderma, and hypertrophic scars, i.e., keloids. Endostatin can be used as a birth control agent by preventing vascularization required for blastocyst implantation and for development of the placenta, the blastocyst, the embryo and the fetus. The synthetic peptide fragments of endostatin have a variety of uses. The peptide that binds to the endostatin receptor with high specificity and avidity is radiolabeled and employed for visualization and quantitation of binding sites using autoradiographic and membrane binding techniques. This application provides important diagnostic and tesearch tools. Knowledge of the binding properties of the endostatin receptor facilitates investigation of the transduction mechanisms linked to the receptor In addition, labeling these peptides with short lived isotopes enables visualization of receptor binding sites in vivo using positron emission tomography or other modern ladiographic techniques in order to locate tumors with endostatin binding sites. Systematic substitution of ammo acids within these synthesized peptides yields high affinity peptide agonists and antagonists to the endostatin receptor that enhance or dim ish endostatin binding to its receptor Such agonists are used to suppress the growth of micrometastases, thereby limiting the spread of cancer. Antagonists to endostatin are applied in situations of inadequate vasculaπzation, to block the inhibitory effects of angiostatin and possibly promote angiogenesis This treatment may have therapeutic effects to promote wound healing in diabetics Endostatin peptides are employed to develop affinity columns for isolation of the endostatin receptor from cultured tumor cells Isolation and purification of the endostatin receptor is followed by amino acid sequencing Next, nucleotide probes are developed for insertion into vectors for expression of the receptor. These techniques are well known to those skilled in the art. Transfection of the endostatin receptor into tumor cells enhances the responsiveness of these cells to endogenous or exogenous endostatin and thereby decrease the rate of metastatic growth. Cytotoxic agents, such as πcm, are linked to endostatin, and high affinity endostatin peptide fragments, thereby providing a tool for destruction of cells that bind endostatin. These cells may be found in many locations, including but not limited to, micrometastases and primary tumors. Peptides linked to cytotoxic agents are infused in a manner designed to maximize delivery to the desired location. For example, πcm-hnked high affinity endostatin fragments are delivered through a cannula into vessels supplying the target site or directly into the target. Such agents are also delivered in a controlled manner through osmotic pumps coupled to infusion cannulae A combination of endostatin antagonists may be co-apphed with stimulators of angiogenesis to increase vasculanzation of tissue. This therapeutic regimen provides an effective means of destroying metastatic cancer. According to the present invention, endostatin may be used in combination with other compositions and procedures for the treatment of diseases. For example, a tumor may be treated conventionally with surgery, radiation or chemotherapy combined with endostatin and then endostatin may be subsequently administered to the patient to extend the dormancy of micrometastases and to stabilize any residual pπmary tumor. The endostatin of the present invention also can be used to generate antibodies that are specific for the inhibitor. The antibodies can be either polyclonal antibodies or monoclonal antibodies. These antibodies that specifically bind to the endostatin can be used in diagnostic methods and kits that are well known to those of ordinary skill in the art to detect or quantify the endostatin in a body fluid or tissue. Results from these tests can be used to diagnose or predict the occunence or recurrence of a cancer and other angiogenesis mediated diseases The endostatin also can be used m a diagnostic method and kit to detect and quantify antibodies capable of binding endostatin These kits would permit detection of circulating endostatin antibodies which indicates the spread of micrometastases in the presence of endostatin secreted by pπmary tumors in situ Patients that have such circulating anti- endostatin antibodies may be more likely to develop tumors and cancers, and may be more likely to have recurrences of cancer after treatments or periods of remission. The Fab fragments of these anti- endostatin antibodies may be used as antigens to generate anti- endostatin Fab-fragment antisera which can be used to neutralize the removal of circulating endostatin by anti- endostatin antibodies. Another aspect of the present invention is a method of blocking the action of excess endogenous endostatin. This can be done by passively immunizing a human or animal with antibodies specific for the undesired endostatin in the system. This treatment can be important in treating abnormal ovulation, menstruation and placentation, and vasculogenesis. This provides a useful tool to examine the effects of endostatin removal on metastatic processes. The Fab fragment of endostatin antibodies contains the binding site for endostatin. This fragment is isolated from endostatin antibodies using techniques known to those skilled in the art. The Fab fragments of endostatin antisera are used as antigens to generate production of anti-Fab fragment serum. Infusion of this antiserum against the Fab fragments of endostatin prevents endostatin from binding to endostatin antibodies. Therapeutic benefit is obtained by neutralizing endogenous anti- endostatin antibodies by blocking the binding of endostatin to the Fab fragments of anti- endostatin The net effect of this treatment is to facilitate the ability of endogenous circulating endostatin to reach target cells, thereby decreasing the spread of metastases. It is to be understood that the present invention is contemplated to include any derivatives of the endostatin that have endothelial inhibitory activity. The present invention includes the entire endostatin protein, derivatives of the endostatin protein and biologically-active fragments of the endostatin protein. These include proteins with endostatin activity that have amino acid substitutions or have sugars or other molecules attached to amino acid functional groups. The present invention also includes genes that code for endostatin and the endostatin receptor, and to proteins that are expressed by those genes. The proteins and protein fragments with the endostatin activity described above can be provided as isolated and substantially purified proteins and protein fragments in pharmaceutically acceptable formulations using formulation methods known to those of ordinary skill m the art. These formulations can be administered by standard routes. In general, the combinations may be administered by the topical, transdermal, intraperitoneal, mtracranial, lntracerebroventπcular, intracerebral, mtravaginal, intrauterme, oral, rectal or parenteral (e.g., intravenous, mtraspinal, subcutaneous or intramuscular) route. In addition, the endostatin may be incorporated into biodegradable polymers allowing for sustained release of the compound, the polymers being implanted in the vicinity of where drug delivery is desired, for example, at the site of a tumor or implanted so that the endostatin is slowly released systemically. Osmotic minipumps may also be used to provide controlled delivery of high concentrations of endostatin through cannulae to the site of interest, such as directly into a metastatic growth or into the vascular supply to that tumor. The biodegradable polymers and their use are described, for example, in detail in Brem et al., /. Neurosurg. 14A41-446 (1991), which is hereby incorporated by reference in its entirety. The dosage of the endostatin of the present invention will depend on the disease state or condition being treated and other clinical factors such as weight and condition of the human or animal and the route of administration of the compound. For treating humans or animals, between approximately 0.5 mg/kilogram to 500 mg/kilogram of the endostatin can be administered. A more preferable range is 1 mg/kilogram to 100 mg/kilogram with the most preferable range being from 2mg/kιlogram to 50 mg/kilogram. Depending upon the half-life of the endostatin in the particular animal or human, the endostatin can be administered between several times per day to once a week. It is to be understood that the present invention has application for both human and veterinary use. The methods of the present invention contemplate single as well as multiple administrations, given either simultaneously or over an extended peπod of time. The endostatin formulations include those suitable for oral, rectal, ophthalmic (including intravitreal or intracameral), nasal, topical (including buccal and subhngual), intrauterme, vaginal or parenteral (including subcutaneous, intraperitoneal, intramuscular, intravenous, tradermal, mtracranial, mtratracheal, and epidural) administration. The endostatin formulations may conveniently be presented in unit dosage form and may be prepared by conventional pharmaceutical techniques. Such techniques include the step of bringing into association the active ingredient and the pharmaceutical carπer(s) or excιpιent(s). In general, the formulations are prepared by uniformly and intimately bringing into association the active ingredient with liquid carriers or finely divided solid earners or both, and then, if necessary, shaping the product. Formulations suitable for parenteral administration include aqueous and non- aqueous sterile injection solutions which may contain anti-oxidants, buffers, bactenostats and solutes which render the formulation isotonic with the blood of the intended recipient; and aqueous and non-aqueous sterile suspensions which may include suspending agents and thickening agents. The formulations may be presented in unit- dose or multi-dose containers, for example, sealed ampules and vials, and may be stored m a freeze-dried (lyophilized) condition requiring only the addition of the sterile liquid carrier, for example, water for injections, immediately prior to use. Extemporaneous injection solutions and suspensions may be prepared from sterile powders, granules and tablets of the kind previously descπbed. Preferred unit dosage formulations are those containing a daily dose or unit, daily sub-dose, as herein above recited, or an appropriate fraction thereof, of the administered ingredient. It should be understood that in addition to the ingredients, particularly mentioned above, the formulations of the present invention may include other agents conventional in the art having regard to the type of formulation in question. Different peptide fragments of the intact endostatin molecule can be synthesized for use in several applications including, but not limited to the following; as antigens for the development of specific antisera, as agonists and antagonists active at endostatin binding sites, as peptides to be linked to cytotoxic agents for targeted killing of cells that bind endostatin. The amino acid sequences that comprise these peptides are selected on the basis of their position on the exterior regions of the molecule and are accessible for binding to antisera. The amino and carboxyl termini of endostatin, as well as the mid- region of the molecule are represented separately among the fragments to be synthesized. The amino terminus distal to the 20th amino acid and carboxyl termini of endostatin may contain or be modified to contain tyrosine and lysme residues and are labeled with many techniques. A tyrosine or lysme is added to fragments that do not have these residues to facilitate labeling of reactive amino and hydroxyl groups on the peptide. These peptide sequences are compared to known sequences using sequence data banks to determine potential sequence homologies. This information facilitates elimination of sequences that exhibit a high degree of sequence homology to other molecules, thereby enhancing the potential for high specificity in the development of antisera, agonists and antagonists to endostatin. Peptides can be synthesized in a standard microchemical facility and purity checked with HPLC and mass spectrophotometry. Methods of peptide synthesis, HPLC puπfication and mass spectrophotometry are commonly known to those skilled in these arts. Peptides and endostatin are also produced in recombinant E. coli, as described below, or in insect or yeast expression systems, and purified with column chromatography. Endostatin and endostatin derived peptides can be coupled to other molecules using standard methods. The amino terminus distal to the 20th amino acid and the carboxyl terminus of endostatin may both contain tyrosine and lysme residues and are isotopically and nonisotopically labeled with many techniques, for example radiolabehng using conventional techniques (tyrosine residues- chloramine T, lodogen, lactoperoxidase: lysine residues- Bolton-Hunter reagent). These coupling techniques are well known to those skilled in the art. The coupling technique is chosen on the basis ot the functional groups available on the amino acids including, but not limited to ammo, sulfhydral, carboxyl, amide, phenol, and lmidazole Various reagents used to effect these couplings include among others, glutaraldehyde, diazotized benzidine, carbodiimide, and p-benzoqumone. Endostatin peptides are chemically coupled to isotopes, enzymes, earner proteins, cytotoxic agents, fluorescent molecules and other compounds for a variety of applications. The efficiency of the coupling reaction is determined using different techniques appropriate for the specific reaction. For example, radiolabehng of an 125 " 5 endostatin peptide or protein with I is accomplished using chloramine T and Na I of high specific activity The reaction is terminated with sodium metabisulfite and the mixture is desalted on disposable columns The labeled peptide is eluted from the column and fractions are collected Aliquots are removed from each fraction and radioactivity measured in a gamma counter. In this manner, the unreacted Na 1 ? 5 I is separated from the labeled endostatin peptide. The peptide fractions with the highest specific radioactivity are stored for subsequent use such as analysis of the ability to bind to endostatin antisera. Another application of peptide conjugation is for production of polyclonal antisera. For example, endostatin peptides containing lysine residues are linked to purified bovine serum albumin using glutaraldehyde. The efficiency of the reaction is determined by measuring the incorporation of radiolabeled peptide. Unreacted glutaraldehyde and peptide are separated by dialysis. The conjugate is stored for subsequent use. Antiserum against endostatin can be generated. After peptide synthesis and purification, both monoclonal and polyclonal antisera are raised using established techniques known to those skilled in the art. For example, polyclonal antisera may be raised in rabbits, sheep, goats or other animals. Endostatin peptides conjugated to a earner molecule such as bovine serum albumin, or endostatin itself, is combined with an adjuvant mixture, emulsified and injected subcutaneously at multiple sites on the back, neck, flanks, and sometimes in the footpads. Booster injections are made at regular intervals, such as every 2 to 4 weeks. Blood samples are obtained by venipuncture, for example using the marginal ear veins after dilation, approximately 7 to 10 days after each injection. The blood samples are allowed to clot overnight at 4C° and are centπfuged at approximately 2400 X g at 4C° for about 30 minutes. The serum is removed, a quoted, and stored at 4C° for immediate use or at -20 to -90C° for subsequent analysis. All serum samples from generation of polyclonal antisera or media samples from production of monoclonal antisera are analyzed for determination of titer. Titer is established through several means, for example, using dot blots and density analysis, and also with precipitation of radiolabeled peptide-antibody complexes using protein A, secondary antisera, cold ethanol or charcoal-dextran followed by activity measurement with a gamma counter The highest titer antisera are also purified on affinity columns which are commercially available Endostatin peptides are coupled to the gel in the affinity column. Antiserum samples aie passed through the column and anti- endostatin antibodies remain bound to the column These antibodies aie subsequently eluted, collected and evaluated for determination of titer and specificity The highest titer endostatin antisera is tested to establish the following, a) optimal antiserum dilution for highest specific binding of the antigen and lowest non-specific binding, b) the ability to bind increasing amounts of endostatin peptide in a standard displacement curve, c) potential cross-reactivity with related peptides and proteins, including endostatin related species, d) ability to detect endostatin peptides in extracts of plasma, uπne, tissues, and in cell culture media. Kits for measurement of endostatin are also contemplated as part of the present invention Antisera that possess the highest titer and specificity and can detect endostatin peptides in extracts of plasma, urine, tissues, and in cell culture media are further examined to establish easy to use kits for rapid, reliable, sensitive, and specific measurement and localization of angiostatin. These assay kits include but are not limited to the following techniques; competitive and non-competitive assays, radioimmunoassay, bioluminescence and chemiluminescence assays, fluorometπc assays, sandwich assays, lmmunoradiometπc assays, dot blots, enzyme linked assays including ELISA, microtiter plates, antibody coated strips or dipsticks for rapid monitoring of urine or blood, and immunocytochemistry For each kit, the range, sensitivity, precision, reliability, specificity and reproducibi ty of the assay are established. Intraassay and interassay variation is established at 20%. 50% and 80% points on the standard curves of displacement or activity One example of an assay kit commonly used in research and in the clinic is a radioimmunoassay (RIA) kit. An endostatin RIA is illustrated below After successful radioiodination and purification of endostatin or an endostatin peptide, the antiserum possessing the highest titer is added at several dilutions to tubes containing a relatively constant amount of radioactivity, such as 10,000 cpm, in a suitable buffer system Other tubes contain buffer or pre-immune serum to determine the non-specific binding. After incubation at 4C° for 24 hours, protein A is added and the tubes are vortexed, incubated at room temperature for 90 minutes, and centnfuged at approximately 2000 - 2500 X g at 4C° to precipitate the complexes of antibody bound to labeled antigen. The supernatant is removed by aspiration and the radioactivity in the pellets counted in a gamma counter. The antiserum dilution that binds approximately 10 to 40 % of the labeled peptide after subtraction of the non-specific binding is further charactenzed. Next, a dilution range (approximately OJ pg to 10 ng) of the endostatin peptide used for development of the antiserum is evaluated by adding known amounts of the peptide to tubes containing radiolabeled peptide and antiserum. After an additional incubation period, for example, 24 to 48 hours, protein A is added and the tubes centnfuged, supernatant removed and the radioactivity in the pellet counted. The displacement of the binding of radiolabeled endostatin peptide by the unlabeled endostatin peptide (standard) provides a standaid curve. Several concentrations of other endostatin peptide fragments, plasmmogen, endostatin from different species, and homologous peptides are added to the assay tubes to characterize the specificity of the endostatin antiserum. Extracts of various tissues, including but not limited to, primary and secondary tumors, Lewis lung carcinoma, cultures of endostatin producing cells, placenta, uterus, and other tissues such as brain, liver, and intestine, are prepared using extraction techniques that have been successfully employed to extract endostatin. After lyophihzation or Speed Vac of the tissue extracts, assay buffer is added and different ahquots are placed into the RIA tubes. Extracts of known endostatin producing cells produce displacement curves that are parallel to the standard curve, whereas extracts of tissues that do not produce endostatin do not displace radiolabeled endostatin from the endostatin antiserum. In addition, extracts of uπne, plasma, and cerebrospinal fluid from animals with Lewis lung carcinoma are added to the assay tubes in increasing amounts. Parallel displacement curves indicate the utility of the endostatin assay to measure endostatin tissues and body fluids Tissue extracts that contain endostatin are additionally characterized by subjecting ahquots to reverse phase HPLC. Eluate fractions are collected, dned in Speed Vac, reconstituted in RIA buffer and analyzed in the endostatin RIA. The maximal amount of endostatin immunoreactivity is located in the fractions conesponding to the elution position of endostatin. The assay kit provides instructions, antiserum, endostatin or endostatin peptide, and possibly radiolabeled endostatin and/or reagents for precipitation of bound endostatin - endostatin antibody complexes. The kit is useful for the measurement of endostatin in biological fluids and tissue extracts of animals and humans with and without tumors. Another kit is used for localization of angiostatin in tissues and cells. This endostatin immunohistochemistry kit provides instructions, endostatin antiserum, and possibly blocking serum and secondary antiserum linked to a fluorescent molecule such as fluorescem isothiocyanate. or to some other reagent used to visualize the primary antiserum. Immunohistochemistry techniques are well known to those skilled in the art. This endostatin immunohistochemistry kit permits localization of endostatin in tissue sections and cultured cells using both light and electron microscopy. It is used for both research and clinical purposes. For example, tumors are biopsied or collected and tissue sections cut with a microtome to examine sites of endostatin production. Such information is useful for diagnostic and possibly therapeutic purposes in the detection and treatment of cancer. This invention is further illustrated by the following examples, which are not to be construed in any way as imposing limitations upon the scope thereof. On the contrary, it is to be clearly understood that resort may be had to various other embodiments, modifications, and equivalents thereof which, after reading the descπption herein, may suggest themselves to those skilled in the art without departing from the spirit of the present invention and/or the scope of the appended claims. Example 1 Identification of an Inhibitor of Capillary Endothelial Cell Proliferation from Heman ψoendothelwma Cells A murine hemangioendothelioma cell line. EOMA (Obeso et al., 1990), was evaluated for evidence of the production of inhibitors of endothelial cell proliferation Many of the known endogenous inhibitors of angiogenesis inhibit the in vitro proliferation of endothelial cells. Conditioned Media Collection. Cells ot the murine hemangioendothelioma cell line EOMA were maintained in DMEM supplemented with 10% bovine calf serum (BCS) and 1 % glutamine-penicillin-streptomycm (GPS) m a 37°C and 10% CO2 incubator. Conditioned media from EOMA cells (1 e. culture media used to grow EOMA cells) was applied to bovine capillary endothelial cells, stimulated with bFGF, in a 72 hour proliferation assay. The conditioned media reversibly inhibited the proliferation of capillary endothelial cells as compared to controls The pattern of inhibition was consistent with the presence of inhibitory and stimulatory activity of endothelial cell proliferation (Figure 1). Example 2 Inhibitory Activity of Endothelial Cell Proliferation is not due to Angiostatin To determine if the inhibitor of capillary endothelial cell proliferation produced by the EOMA cells was angiostatin, pooled conditioned media was applied to a lysine column (lysine conjugated to Sepharose™ chromatography beads). Lysine Sepharose binds angiostatin and has been used for its purification (O'Reilly et al., 1996). The endothelial cell inhibitory activity was found only in the flow through fraction and not in the bound fraction (data not shown) The lack of binding of the inhibitory activity to lysine Sepharose suggested that the novel inhibitor of endothelial cell proliferation was not angiostatin. Example 3 Purification of a 20 kDa Protein from the Conditioned Media of EOMA Cells which Specifically inhibits Endothelial Cell Proliferation Because several angiogenesis inhibitors have an affinity for hepann, we applied the flow-through from the lysine Sepharose column to a hepann Sepharose column. The inhibitory activity bound hepann with relatively high affinity and was eluted with 0.6-0.8 M NaCl in 10 mM Tπs pH 1.4. as shown in Figure 2. To further purify the inhibitory activity, the sample was concentrated and applied to a gel filtration (Bio-Rad Bio-Gel P-100 fine gel or Pharmacia Sephacryl S-200HR gel) column (see Figure 3), followed by several cycles of reverse-phase HPLC with a C4 column. The inhibitory activity was eluted from the C4 column with 40-45% acetonitπle 0.1% tπfluoroacetic acid, as exemplified by Figure 4 After the final C4 column, the inhibitory activity was associated with a protein of molecular mass of approximately 20 kDa (reduced) or 18 kDa (non-reduced), by SDS-PAGE, punfied to apparent homogeneity. With respect to Examples 2 and 3, lysine Sepharose, hepann Sepharose, Sephaciyl S -200 HR gel (Pharmacia, Uppsala, Sweden), Bio-Gel P-100 fine polyacrylamide gel (Bio-Rad Laboratories, Richmond, CA), and a SynChropak RP4 (100 x 4 6 mm) C4 reverse-phase column (Synchrom, Inc., Lafayette, IN) were prepared according to the manufacturers recommendations. A hepann-Sepharose column (50 x 2.5 cm) was equilibrated with 50 mM NaCl 10 mM Tπs-HCl pH 7 4 Pooled conditioned media was applied and the column was washed with the equilibration buffer. The column was eluted with a continuous gradient of 50 mM - 2 M NaCl in 10 mM Tns-HCl at pH 7.4 (200 ml total volume) followed by 100 ml of 2 M NaCl in 10 M Tπs-HCl at pH 7 4. Fractions were collected and an aliquot of each was applied to capillary endothelial cells. Fractions which inhibited their proliferation were dialyzed (MWCO = 6,000-8,000) against PBS and concentrated using a 4000 MWCO Nanosp concentrator (Gelman Sciences, Ann Arbor, Ml). A Bio-Gel P-100 column or a Sephacryl S-200 HR column (75 x 1.5 cm) was equilibrated with PBS. The sample from hepann Sepharose chromatography was applied and the column was fluted with the equilibration buffer. Fractions were collected and an aliquot of each was applied to endothelial cells. Fractions which inhibited endothelial proliferation were concentrated and dialyzed as above. A SynChropak RPG (100 x 4.6mm) column was equilibrated with H2θ/0J% tπfluoroacetic acid (TFA). HPLC-grade reagents (Pierce, Rockford, IL) were used. The sample from gel filtration chromatography was applied to the column and the column was fluted with a gradient of acetonitπle in 0.1% TFA at 0.5 ml/minute and fractions were collected. An aliquot of each was evaporated by vacuum centπfugation, resuspended in PBS, and applied to capillary endothelial cells. Inhibitory activity was further purified to apparent homogeneity by at least two subsequent cycles on the SynChropak C4 column. To further characterize the 20 kDa inhibitor, we tested it on several cell lines of endothelial and non -endothelial ongin. For the BCE assay, bovine capillary endothelial cells were obtained and grown as previously described (Folkman et al., 1979) For the proliferation assay, cells were washed with PBS and dispersed in a 0.05% solution of trypsin. A cell suspension (25,000 cells/ml) was made with DMEM + 10% BCS + 1% GPS, plated onto gelatinized 24-well culture plates (0.5 mewed), and incubated (37°C, 10% CO2) for 24 hours. The media was replaced with 0.25 ml of DMEM + 5% BCS + 1% GPS and the test sample applied. After 20 minutes of incubation, media and bFGF were added to obtain a final volume of 0.5 ml of DMEM + 5% BCS + 1 % GPS + 1 ng/ml bFGF. After 72 hours, cells were dispersed in tryps , resuspended in Hematall (Fisher Scientific, Pittsburgh, PA), and counted by Coulter counter. Non- Endothelial Cell Proliferation Assays Bovine aortic smooth muscle (SMC), bovine retinal pigment epithelial (RPE), mink lung epithelial (MLE), Lewis lung carcinoma (LLC), and EOMA cells and 3T3 fibroblasts were maintained in a 10% CO2 and 37° C incubator. For the proliferation assays, cells were washed with PBS and were dispersed in a 0.05% solution of trypsin Optimal conditions for the cell proliferation assays were established for each different cell type. Fetal calf serum (FCS) was used for the RPE, MLE, and LLC cells and BCS was used for the other cell types. A cell suspension (20,000 cells/ml for SMC, RPE, MLE; 15,000 cells/ml for 3T3; 10,000 cells/ml for LLC, EOMA) was made with DMEM + 10% bovine serum + 1% GPS, plated onto 24-well culture plates (0 5 ml/well), and incubated (37°C, 10% C02) for 24 hours. The media was replaced with 0.5 ml of DMEM + 5% bovme serum + 1 % GPS and the test sample applied. After 72 hours, cells were dispersed in trypsin, resuspended in Hematall (Fisher Scientific, Pittsburgh, PA), and counted by Coulter counter. Only endothelial cells were significantly inhibited, as shown in Table 2. TABLE 2 EFFECT OF ENDOSTATIN ON ENDOTHELIAL AND NON-ENDOTHELIAL CELL PROLIFERATION The inhibition was first observed at doses of 100 ng/ml with maximal inhibition observed at doses of 600 ng/ml or greater. No significant inhibition was seen for cells of non-endothehal origin at doses 1 log unit higher than those used to inhibit capillary endothelial cell proliferation (data not shown). Example 4 Microsequence Analysis of the 20 kDa Protein Reveals Identity to a Fragment of Collagen XVIII The 20 kDa inhibitor of capillary endothelial cell proliferation from the conditioned media was purified to homogeneity, as described in the above examples, resolved by SDS-PAGE, electroblotted onto PVDF (Bio-Rad, Richmond. CA), detected by Ponceau S stain, and excised fi om the membrane. N-terminal sequence was determined by automated Edman degradation on a PE/ABD Model 470A protein sequencer (Foster City, CA) operated with gas-phase delivery of tπfluoracetic acid. Sequence library searches and alignments were performed against combined GenBank, Brookhaven Protein, SWISS-PROT, and PIR databases. Searches were performed at the National Center for Biotechnology Information through the use of the BLAST network service. Microsequence analysis of the inhibitor revealed identity to a C-termmal fragment of collagen XVIII. The molecular cloning and sequence of collagen XVIII was first described by Olsen and his coworkers and by Rehn and Pihlajaniemi (Oh et al., 1994; Rehn and Pihlajaniemi, 1994) Collagen XVIII is a novel collagen which consists of an N-termmal region with 3 splice variants (Muragaki et al., 1995 : Rehn and Pihlajaniemi, 1995), a senes of collagen-like domains with intenuptions. and a 35 kDa C-terminal non-collagenous (NC I ) domain An 18-amιno acid N-terminal microsequence analysis of the purified inhibitor of endothelial cell proliferation confirms that it is identical to a C-terminal fragment of this NCI domain (Figure 5) We have named this inhibitory fragment of collagen XVIII "endostatin" and it is included in the group of molecules that have endostatin activity. Example 5 Recombinant Mouse Endostatin (Baculovirus or E. coli) Inhibits Endothelial Cell Proliferation In Vitro and Angiogenesis In Vivo The endothelial proliferation cell inhibitor of the present invention can be recombinantly expressed in any system used to express proteins. Non-limiting examples of such expressions systems include bacterial expression systems, yeast expression systems and insect viral expression systems. Recombinant mouse endostatin was expressed using the BacPAK baculovirus expression system (CLONTECH Laboratories) following the manufacturer's protocol. Briefly, a cDNA fragment encoding the signal sequence and C-termmal part (endostatin region) of mouse collagen XVIII was inserted into the pBacPAK8 transfer vector BacPAKό viral DNA (expression vector) and plasmid DNA of the pBacPAKδ-endostatm clone (modified transfer vector) were then co-transfected into insect Sf21 cells and media containing expressed mouse endostatin was collected. The BacPAKό was first digested with BSU36 enzyme to make it incompetent for independent replication. The media containing expressed mouse endostatin was applied to a 1.5 x 40 cm hepann Sepharose column which had been equilibrated with 50 mM NaCl 10 mM Tπs pH 1.4. The column was washed with the equilibration buffer and was then eluted sequentially with 0.2 M NaCl, 0.4 M NaCl, 0.6 M NaCl, and 1 M NaCl m 10 mM Tns pH 7 4 All chromatography was performed at 4° C The 0 6 M NaCl eluant (which inhibited bovine capillary endothelial cells in a 72 hour prolifei ation assay) was dialyzed (6-8000 MWCO) against PBS and then reapp ed to the hepann Sepharose column. The column was eluted with a gradient of 50 mM NaCl - 1 2 M NaCl in 10 mM Tπs pH 7 4 An aliquot of each fraction was applied to bov e capillary endothelial cells as above and fractions which inhibited proliferation were pooled, dialyzed against PBS, and concentrated using a Nanospin Plus (Gelman Sciences) centrifugal concentrator (MWCO = 10,000). SDS -PAGE of the concentrated sample revealed a discrete band of apparent M r of 20 kDa. Expression and Purification of Recombinant Mouse Endostatin from E. coli The C -terminal part of the cDNA of collagen XVIII was used to amplify the cDNA of mouse endostatin which was cloned into the pETKHl vector (pETl ld deπvative) (Studier et al., 1990). Induction resulted in the production of a fusion protein canying the amino acid sequence MARRASVGTD (SEQ ID NO:2) (RRAS = protein kinase A recognition sequence) and 6 histidine residues at the N-termmus followed by the sequence of mouse endostatin (pTB01#8) The pTB01#8 plasmid was transformed into BL2LDE3 and the fusion protein was purified on Nι+2-.NT Vbeads as described (QiaExpressionist Handbook, Qiagen) Briefly, E. coli were grown until an O.D.600 of 0.8 - 0.9 and expression of the fusion protein was then induced for 3 hours with 1 mM IPTG. The bacteria were pelleted and resuspended in 8 M urea, 10 mM Tns-HCl pH 8.0 containing 10 mM lmidazole and incubated for 1 hour at room temperature. The suspension was centnfuged for 15 minutes at 20,000 g and the supernatant incubated with the Nι + 2-NTA beads for 1 hour at room temperature The suspension was transfened into a column and washed with 8 M urea, 0J M Na-phosphate, 10 mM Tns- HCl pH 6.25 containing 10 mM lmidazole. The protein was eluted with the same buffer containing 250 mM lmidazole. The fractions containing endostatin were extensively dialyzed against PBS. During dialysis, the endostatin precipitated. The precipitated endostatin was resuspended in PBS, the protein concentration was adjusted to 2 - 4 mg/ml, and the endostatin was stored at -20° C until use. For the mouse studies, endostatin was delivered as a suspension in PBS For the chick choπoallantoic assay, endostatin was further dialyzed against water and then lyophilized. Recombinant mouse endostatin was produced in both baculovirus and E. coli expression systems. Using sequential hepann Sepharose chromatography, recombinant mouse endostatin was purified to apparent homogeneity from insect cell media. Nι + 2-NTA-agarose was used to punfy the E. cσ/i-deπved mouse endostatin. SDS-PAGE revealed a discrete band of approximately 20 kDa or approximately 22 kDa (reduced) purified to apparent homogeneity for baculovirus and E. cø/.-deπved recombinant endostatins. respectively (data not shown). Both were dialyzed against PBS prior to use After dialysis, the material from the E. coli system precipitated and was delivered as a suspension for subsequent in vivo studies. Recombinant endostatin from baculovirus specifically inhibited the proliferation of bovine capillary endothelial cells in a dose-dependent fashion The inhibition was seen at doses of 100 ng/ml with maximal inhibition observed at doses above 600 ng/ml No significant inhibition of proliferation of cells of non-endothelial origin or of the EOMA cells was observed when endostatin was tested at doses up to one log unit higher than those used to inhibit endothelial cell proliferation The precipitated (un-refolded) material was not testable in vitro, because of its insolubility. However, a small percentage was soluble in PBS during dialysis and this fraction was used for the endothelial cell assays Furthermore, after refolding, it was soluble and inhibited endothelial proliferation (data not shown) When this soluble material was applied to endothelial cells, it was found to be inhibitory at concentrations comparable to both the native and baculovirus-deπved endostatin. To test for the ability of recombinant mouse endostatin to inhibit in vivo angiogenesis, we used the chick choπoallantoic membrane (CAM) assay (Folkman, 1985; Nguyen et al., 1994 which are incorporated herein by reference). Bnefly, three day old fertilized white Leghorn eggs (Spafas, Norwich, CT) were cracked, and embryos with intact yolks were placed in 100 x 20 mm petπ dishes (Folkman, 1985). After 3 days of incubation (37° C and 3% CO2), a methylcellulose (Fisher Scientific, Fair Lawn, N.J.) disc containing endostatin was applied to the CAM of individual embryos. The discs were made by desiccation of endostatin in 10 μl of 0.45% methylcellulose (in H2O) on teflon rods. After 48 hours of incubation, embryos and CAMs were observed by means of a stereomicroscope. At doses of 10-20 μ.g/10 μl disc, there was potent inhibition of in vivo angiogenesis for both the E. coli and the baculovirus-deπved endostatins in all of the tested CAMs (n=5/group). The E. coli deπved-endostatm precipitate gradually dissolved over 5 days and produced a sustained anti angiogenic effect on the implanted CAMs. In contrast, the soluble baculovirus-denved endostatin dissolved within 24 hours and gave a maximal antiangiogenic effect within a period of 48 hours. There was no evidence of toxicity in any of the chick embryos tested. Human Endostatin was produced recombmantly using similar methods. Example 6 Recombinant Mouse Endostatin Inhibits the Growth of Metastases Because tumor growth is angiogenesis dependent, we treated Lewis lung carcinoma metastases systematically with recombinant mouse endostatin expressed in the baculovirus system Animals with Lewis lung carcinomas of 600-1200 mm^ tumors were sacrificed and the skm overlying the tumor was cleaned with betadine and ethanol. In a laminar flow hood, tumor tissue was excised under aseptic conditions. A suspension of tumor cells in 0 9% normal sal e was made by passage of viable tumor tissue through a sieve and a series of sequentially smaller hypodermic needles of diameter 22- to 30- gauge The final concentration was adjusted to 1 x 107 cells/ml and the suspension was placed on ice. After the site was cleaned with ethanol, the subcutaneous dorsa of mice in the proximal mid ne were injected with 1 x 106 cells in 0J ml of saline When tumors were 1500 mm^ m size, approximately 14 days after implant, the mice underwent surgical removal of the tumor The incision was closed with simple interrupted sutures From the day of operation, mice received daily intraperitoneal injections of recombinant (baculovirus) mouse endostatin or saline. Mice received 0.3 mg/kg/day of endostatin once daily via subcutaneous injection When the control mice became sick from metastatic disease (i.e., after 13 days of treatment), all mice were sacrificed and autopsied. Lung surface metastases were counted by means of a stereomicroscope at 4x magnification. The growth of Lewis lung carcinoma metastases was almost completely suppressed by the systemic administration of endostatin at a dose of 0.3 mg/kg/day given subcutaneously (7 ± 3 metastases/mouse, n=4, p < 0.001). In contrast, in mice treated with saline after removal of a Lewis lung carcinoma primary tumor, lung metastases grew rapidly (77 ± 7 metastases/mouse). Lung weight, which reflects tumor burden, was 240 ± 25 mg in the endostatin treated mice versus 760 ± 30 mg in the control mice (p < 0.001). Further, there was no weight loss or evidence of toxicity in any of the mice treated with endostatin Example 7 Recombinant Mouse Endostatin Inhibits the Growth of Primary Tumors The yield of endostatin from the baculovirus system was lower than that of the E. coli system, i.e. 1-2 mg/liter versus 30-40 mg/liter. We therefore used E. co/z-derived endostatin to study the effect of endostatin therapy on primary tumor growth. We produced recombinant mouse endostatin from E. coli in sufficient quantity to treat Lewis lung carcinoma pπmary tumors. The endostatin was administered as a suspension of the precipitated purified protein to mice bearing Lewis lung carcinomas of at least 100-200 mm The protein was purified by conventional means but was not refolded prior to its administration to the mice. The injected precipitate was slowly resorbed over 24-48 hours. We are unaware of any piecedent for the use of an injected depot of non -refolded recombinant protein as a sustamed-ielease method in animals. Nevertheless, endostatin gradually resorbed in vivo and proved to have potent antiangiogenic activity which resulted in prolonged anti-tumor and antiangiogenic activity Therefore, these data suggest a novel general method for the controlled release of recombinant proteins Based on this rationale, we have delivered non-refolded recombinant angiostatin fiom E coli with similar success Accordingly, an aspect of the invention is the administration ot recombinant endostatin or endostatin analogs in an un-refolded state so as to provide a sustained release depot of endothelial cell proliferation inhibiting protein over a period of at least 8 hours, desirably at least 12 hours, more desirably at least 24 hours or at least 48 hours, depending on the patient and the disease to be treated. Optionally recombinant and un- refolded angiostatin is administered to similarly provide a sustained release depot of protein capable of releasing angiostatin protein over a penod of at least 8 hours, desirably at least 12 hours, more desirably at least 24 hours or at least 48 hours, depending on the patient and the disease to be treated. Mice were implanted with Lewis lung carcinomas as described above. Tumors were measured with a dial-cahper and tumor volumes were determined using the formula width^ x length x 0.52, and the ratio of treated to control tumor volume (T/C) was determined for the last time point. After tumor volume was 100-200 mm^ (0.5 - 1% of body weight), which occuned within 3-7 days, mice were randomized into two groups One group received recombinant mouse endostatin (E. coli) as a suspension in PBS injected subcutaneously at a site distant from the tumor once daily. The other group received comparable injections of the vehicle alone. The experiments were terminated and mice were sacrificed and autopsied when the control mice began to die. The growth of Lewis lung primary tumors was potently suppressed by systemic therapy with endostatin. Increasing the dose of endostatin was associated with improved efficacy (data not shown). At a dose of 10 mg/kg, tumor growth was inhibited by 97% as compared to control mice treated with vehicle alone. At a dose of 20 mg/kg given once daily, m two separate experiments, there was an almost complete regression of established primary tumors (>99% inhibition, p <0.001). These surprising and unexpected results are shown in Figures 6 and 7. Figures 8, 9, 10 and 11 demonstrate the effectiveness of recombinant mouse endostatin for inhibiting tumor growth in a variety of different tumor models. Also demonstrated is the effectiveness of endostatin derived from human for inhibiting tumor growth. Immunohistochemical analysis (Figure 12) of the residual small tumors showed a potent inhibition of angiogenesis in the endostatin treated tumors Further, the proliferative index of tumors in the endostatin and saline treated mice was at the same high level in both groups while the apoptotic index increased 8-fold after endostatin therapy. Thus, endostatin therapy results in a similar pattern of tumor dormancy to the one we have previously described for angiostatin (Holmgren et al., 1995; O'Reilly et al , 1996). Further, there was no evidence of drug-i elated toxicity in any of the treated mice After discontinuation of endostatin therapy, a tumor iecuπed at the primary site within 5-14 days, became vasculaπzed. and eventually killed the mice (data not shown) Notably, we found that E to/.-deπved recombinant mouse endostatin with a C-terminal polyhistidine tag, which was expressed purified and administered in a comparable fashion to the N-terminal tagged product described above did not inhibit angiogenesis in the CAM assay and had no effect on the growth of Lewis lung carcinomas (data not shown). These data argue strongly that the anti -tumor and antiangiogenic activity of recombinant endostatin are due to the specific structure of endostatin and not to a contaminant in the sample Figure 13 shows the results of cycled treatment of Lewis lung carcinoma with recombinant mouse endostatin derived from E coli These results clearly show reproducible endostatm-dependent regression of tumor mass, followed by tumor growth after termination of endostatin treatment. These results show that a murine hemangioendothelioma produces a novel and specific 20 kDa inhibitor of endothelial cell proliferation in vitro which is also a potent inhibitor of angiogenesis and tumor growth in vivo. The N-terminal sequence of this inhibitor, endostatin, is identical to a C-terminal fragment of collagen XVIII. Systemic administration of recombinant endostatin potently inhibits angiogenesis, maintains metastases at a microscopic size, and regresses primary tumors to less than 1 mrc , a reduction of over 150-fold For as long as mice are treated there is no re-growth of tumors, no evidence of drug resistance, and no toxicity. It is interesting to note that some fragments of the C -terminal domain of collagen type XVIII that are longer than endostatin do not inhibit endothelial cell proliferation (data not shown). Endostatin was discovered by the same strategy employed to find angiostatin (O'Reilly et al.J994), i.e , isolation from a tumor. While it is counter-intuitive that tumors should be a source of angiogenesis inhibitors, the results reported here seem to validate this approach. This leads to the question of why angiogenesis inhibitors should be present in tumors that are angiogenic One possibility is that an inhibitor could be 'left-over' after down-regulation of its production by a tumor cell undergoing the switch to the angiogenic phenotype. This appears to be the case for thrombospondin produced by Li-Fraumeni cells in which the second allele for p53 is mutated or deleted (Dameron et al., 1994) A second possibility is that the proteolytic activity which accompanies tumor growth, and which is an important component of capillary blood vessel growth, may also mobilize circulating angiogenesis inhibitors from precursor proteins which are not inhibitory themselves. Angiostatin for example, inhibits angiogenesis and endothelial cell proliferation while plasmmogen does not (O'Reilly et al., 1996. O'Reilly et al., 1994). For endostatin, a similar pattern is revealed. Histology of tumors which regressed under endostatin therapy showed peπvascular cuffing of tumor cells surrounding one or more microvessels in which angiogenesis was blocked. Tumor cells displayed high proliferation balanced by high apoptosis, with no net gam in tumor size. These data are consistent with a model of a new type of tumor dormancy recently proposed (Holmgren et al., 1995). Furthermore, endostatin inhibited proliferation of endothelial cells in vitro, but had no effect on Lewis lung carcinoma cells, or other cell types including smooth muscle, epithelium, fibroblasts, and the EOMA cell line from which it was puπfied. The fact that a specific inhibitor of endothelial cell proliferation can regress a tumor to a microscopic size and hold it in a dormant state, despite the fact that the tumor cells are refractory to the inhibitor from the outset, indicates that the endothelial population can exert powerful growth regulatory control over the tumor cells. The results with endostatin support the theory (Folkman, 1996) that for therapeutic purposes, it is fruitful to think about a tumor m terms of two distinct cell populations: a tumor cell population and an endothelial cell population, each of which can stimulate growth of the other. Growth of each cell population may be optimally inhibited by agents which selectively or specifically target that cell type, i.e., cytotoxic chemotherapy and antiangiogenic therapy. Furthermore, combined treatment of both cell populations may be better than treatment of either cell type alone. To test this theory mice seeded with Lewis lung carcinomas, and beanng tumors which had attained a size of approximately 300 mm^, were treated with a combination therapy comprising angiostatin and endostatin, each at a dose of 20 mg/kg/day for 25 days. Tumors regressed to microscopic levels by about day 10 of treatment. A completely unexpected finding was that tumors remained regressed and dormant for approximately three months, even after all treatment was terminated, as is shown in Figure 14 Experiments of longer duration indicate that an initial treatment of tumor with a combination of angiostatin and endostatin causes a very long term dormancy, the actual penod of which is unknown at this time. Such long term dormancy is considered a cure to one skilled in the art. For example, the NIH guideline for determining when a treatment is effective as a cancer cure, is that the tumor remain dormant (i.e. not increasing m size) for ten times the normal doubling time of the tumor. The dormancy length achieved using a combination of endostatin and angiostatin far exceeds this cnteπa. Accordingly, an important aspect of the invention is a composition compnsing a combination of angiostatin and endostatin, or an endostatin analog, in amounts sufficient to cause long term dormancy, or cure, of angiogenesis-dependent cancers when administered to patients with angiogenesis-dependent cancers. Administration can be systemically, for example by injection, in which case the dosage is determined depending upon the patient and the particular cancer, but which generally is at least 0.2 mg/kg/day, desirably at least 2 0 mg/kg/day, more desirably at least 20 mg/kg/day Generally, the composition is administered daily for at least 10 days, desirably at least 20 days, more desirably at least 25 days. Alternative systemic administration routes include, orally where the composition is formulated, for example into coated microbeads, to protect the protein from inactivating digestive environments; transdermally; and via pump Alternatively, different dosages and treatment periods can be used if the composition is administered locally to an angiogenesis-dependent site, such as a tumor. Such administration may be, for example, surgical implantation or local injection into, or near by, the site Example 8 Isolation of the putative receptor for endostatin. Both endostatin and angiostatin appear to be specific inhibitors of endothelial cell proliferation. Therefore, it is likely that endostatin binds to specific structures exclusively expressed on the surface of endothelial cells. We are not aware of the existence of any other specific inhibitors of endothelial cell proliferation. Identifying and isolating proteins which specifically bind to endostatin is accompanied by methods well known in the art, for example by affinity chromatography and expression cloning. Affinity chromatography Bovine Capillary Endothelial cells (BCE) are radiolabeled with [ 35 S]-methιonιne, total cell and membrane extracts prepared and applied to affinity columns prepared with endostatin. As a negative control, fibroblast protein extracts are isolated in a similar way. Bound proteins are eluted from the column using a NaCl gradient and the different fractions are analyzed using standard SDS-PAGE and autoradiography. This procedure yields proteins that are tightly bound to the endostatin column and present only in the endothelial cell derived fractions. Comparing the gel electrophoretic patterns of the two cell types reveals expressed proteins unique to the BCE cells. Protein sequences subsequently are determined and conesponding gene(s) cloned. A cDNA library of bovine capillary endothelial cells, is prepared and screened with a degenerative ohgo based PCR technique to locate the cDNA(s) of the endostatin- specific binding protem(s). Hybridization using degenerative oligonucleotides to the conesponding cDNA, is also used to identify genes of endostatin binding proteins. Another approach is to raise antibodies against the peptide sequences with methods descπbed earlier m the Detailed Descnption and immunoscreen the same library. Expression cloning. A cDNA library of BCE cells is prepared. Poly-A mRNA is isolated from BCE cells whose proliferation has previously been inhibited by endostatin. These cells express an endostatin binding protein. The corresponding cDNA library is transfected into cells allowing high expression of the various cDNAs. Binding activity of endostatin to cells which express the receptor protein on the surface is used as a positive selection of these cells. To select for these cells, purified endostatin is labeled with biot and consequently detected using either streptavidin coupled magnetic-beads or FACS sorting. Alternatively, an antibody against endostatin is used for screening. After selection ot the positive cells, the conesponding plasmids are isolated, amplified and transfected again into high expression cells. After several rounds of positive selection, plasmids are analyzed for identical inserts using endonuclease digestion and PCR. Using these data, complementation groups are formed, sequenced and analyzed with the BLAST network program. In addition to computer analysis, individual cDNAs are re-transfected into high expression cells and tested for endostatin binding activity under different conditions (e.g., competition with non-labeled endostatin, time-course of binding, Scatchard analysis, etc. in other words the use of "classical" receptor characterization procedures known to those skilled in the art). Example 9 Determination of the minimal region of the mouse endostatin protein responsible for its antiangiogenic activity. Different PCR pnmers are designed, the conesponding cDNAs cloned into the E. coli expression system, and the different endostatin fragments purified to homogeneity. The full length cDNA is cut from both the N- and C-termmus. As a first screen, the capillary endothelial proliferation assay and the chick embryo assay are used to determine the residual activity compared to the full length fragment. Example 10 Determination of the putative enzyme(s) which may release endostatin from collagen XVIII. Collagen XVIII belongs to the non-fibπllar collagen type family and can be found in three different splicing variants encoding for proteins with 1315-, 1527-, and 1774 - ammo acid residues (Rehn, PNAS 91 :4234, 1994). The difference is caused by alterations in the N-termmal part of the gene and therefore all three splicing variants could potentially be the source of endostatin which itself is a fragment of the non- collagenous domain 11 (NC11) The function of collagen XVIII is not known, but because its message is substantially expressed in highly vascularized organs, a role in peπvascular matrix assembly and/or structure has been proposed (Oh. et al , Genomics. 19:494, 1994). A first clue about the function of collagen XVIII came from the purification of endostatin as a potent inhibitor ot endothelial cell prohfeiation From this preliminary data and from our initial observation that endostatin was purified from conditioned medium of a hemangioendothelioma (EOMA), we asked whether the enzyme(s) which release endostatin from collagen XVIII could be identified The last 325 amino acid residues, encoding for the NC11 domain, are expressed ιn £ coli and the insect cell baculovirus system, the punfied protein is used as a substrate to identify enzymes that clone this region of collagen XVIII By PCR, a cDNA fiagment encoding the NC11 domain is cloned into an E coli expression vector (pET senes) which allows high expression of the target protein after induction with IPTG. Alternatively, a vector suitable for insect cell expression is used. The proteins are tagged with the HIS6-Tag located on the C-terminus for purification using Ni2+-NTA-beads An Nι2+-NTA-alkalιne phosphatase conjugate can detect the C-terminus by Western blotting. Another construct is made which not only has a HISβ-Tag on the C-termmus, but will also encode the hemaglutmin HA-tag on the N-termmus. This is detected by Western blotting with an HA-specific monoclonal antibody. The N- and C-termmus of the protein followed after incubation with EOMA supernatant and different metalloproteinase extracts. Cleavage product is detected by SDS-PAGE analysis or Western blotting, the protein is re -punfied using the Nι^+-NTA beads, eluted with lmidazole, dialyzed against PBS and tested for inhibitor activity in the various in vitro and in vivo assays (e.g., endothelial cell proliferation, chick embryo, and mouse corneal assay) If the purified cleavage product shows inhibitory activity, N-terminal ammo acid sequencing is performed and compared to the original starting sequence of endostatin obtained from the EOMA supernatant. Accordingly, the cleavage procedure can be scaled up to purify sufficient protein for testing in tumor-beanng mice, and to compare this activity to that of the full length NCI 1 domain. Example 11 Sequence of Human Endostatin Protein Based on the data revealed in the above examples, and the publicly available protein sequences of human collagen XVIII (Oh, et al., Genomics, 19:494, 1994), the following is an example of a functional human endostatin protein of the present invention. This is the carboxy terminal protein of human collagen XVIII, starting at the ammo-terminal end position 1132, as conelating to the murine fragment of SEQ ID NOJ above. Human Endostatin protein sequence, 182 aa (SEQ ID NO:3) HSHRDFQPVLHLVALNSPLSGGMRGIRGADFQCFQQARAVGLAGTFRAFLSSRL QDLYSIVRRADRAAVPIVNLKDELLFPSWEALFSGSEGPLKPGARIFSFDGKDVL RHPTWPQKSVWHGSDPNGRRLTESYCETWRTEAPSATGQASSLLGGRLLGQSA ASCHHAYIVLCIENSFMTAS Furthermore, based on publicly available gene sequences for human collagen XVIII, the following is a representative gene encoding for the above endostatin protein. Human Endostatin gene sequence, 546 bp (SEQ ID NO:4) CACAGCCACCGCGACTTCCAGCCGGTGCTCCACCTGGTTGCGCTCAACAGCC CCCTGTCAGGCGGCATGCGGGGCATCCGCGGGGCCGACTTCCAGTGCTTCCA GCAGGCGCGGGCCGTGGGGCTGGCGGGCACCTTCCGCGCCTTCCTGTCCTCG CGCCTGCAGGACCTGTACAGCATCGTGCGCCGTGCCGACCGCGCAGCCGTGC CCATCGTCAACCTCAAGGACGAGCTGCTGTTTCCCAGCTGGGAGGCTCTGTT CTCAGGCTCTGAGGGTCCGCTGAAGCCCGGGGCACGCATCTTCTCCTTTGAC GGCAAGGACGTCCTGAGGCACCCCACCTGGCCCCAGAAGAGCGTGTGGCAT GGCTCGGACCCCAACGGGCGCAGGCTGACCGAGAGCTACTGTGAGACGTGG CGG ACGGAGGCTCCCTCGGCC ACGGGCC AGGCCTCCTCGCTGCTGGGGGGC A GGCTCCTGGGGCAGAGTGCCGCGAGCTGCCATCACGCCTACATCGTGCTCTG CATTGAGAACAGCTTCATGACTGCCTCC As described above, amino acid substitutions may occur in the sequence of endostatin, which still yield a functional endostatin protein For example, when the above gene sequence is recombinantly expressed, an observable doublet of protein results, both versions of which are functional endostatin proteins. In addition to the above endostatin protein, the following endostatin variant occurs, which is the former protein minus the first four amino acids. This demonstrates the variability of functional endostatin protein molecules. Alternate Human Endostatin protein sequence, 178 aa (SEQ ID NO:5) DFQPVLHLVALNSPLSGGMRGIRGADFQCFQQARAVGLAGTFRAFLSSRLQDLY SIVRRADRAAVPIVNLKDELLFPSWEALFSGSEGPLKPGARIFSFDGKDVLRHPT WPQKSVWHGSDPNGRRLTESYCETWRTEAPSATGQASSLLGGRLLGQSAASCH HAYIVLCIENSFMTAS Furthermore, based on publicly available gene sequences for human collagen XVIII, the following is a representative gene encoding for the above alternate endostatin protein. Alternate Human Endostatin gene sequence. 534 bp (SEQ ID NO:6) GACTTCCAGCCGGTGCTCCACCTGGTTGCGCTCAACAGCCCCCTGTCAGGCG GCATGCGGGGCATCCGCGGGGCCGACTTCCAGTGCTTCCAGCAGGCGCGGGC CGTGGGGCTGGCGGGCACCTTCCGCGCCTTCCTGTCCTCGCGCCTGCAGGAC CTGTACAGCATCGTGCGCCGTGCCGACCGCGCAGCCGTGCCCATCGTCAACC TCAAGGACGAGCTGCTGTTTCCCAGCTGGGAGGCTCTGTTCTCAGGCTCTGA GGGTCCGCTGAAGCCCGGGGCACGCATCTTCTCCTTTGACGGCAAGGACGTC CTGAGGCACCCCACCTGGCCCCAGAAGAGCGTGTGGCATGGCTCGGACCCCA ACGGGCGCAGGCTGACCGAGAGCTACTGTGAGACGTGGCGGACGGAGGCTC CCTCGGCC ACGGGCC AGGCCTCCTCGCTGCTGGGGGGC AGGCTCCTGGGGC A GAGTGCCGCGAGCTGCCATCACGCCTACATCGTGCTCTGCATTGAGAACAGC TTCATGACTGCCTCC References The following references are hereby incorporated by reference herein in their entirety. Angiohllo, A. L., Sgadaπ, C, Taub, D. D., Liao, F., Farber, J. M., Miaheshwaπ, S., Kleinman, H. K., Reaman, G. H., and Tosato, G. (1995). Human mterferon-inducible protein 10 is a potent inhibitor of angiogenesis in vivo. J. Exp. Med. 182, 155-162. Cao, Y., Chen, C, Weatherbee, J. A., Tsang, M., and Folkman, J. (1995). Gro-beta, a C-X-C chemok e, is an angiogenesis inhibitor that suppresses the growth of Lewis lung carcinoma in mice. J. Exp. Med. 182, 2069-2077. Chen, C, Parangi, S.. Tolentino, M. J., and Folkman, J (1995). A strategy to discover circulating angiogenesis inhibitors generated by human tumors. Cancer Res. 55, 4230-4233. Clapp, C, Martial, J. A., Guzman, R. C., Rentier-Delrue, F., and Werner, R. 1. (1993) The 16-kilodalton N-terminal fragment of human prolactin is a potent inhibitor of angiogenesis. Endocnnology 133, 1292-1299. Dameron, K. M., Volpert, O. V., Tainsky, M. A., and Bouck, N. (1994). Control of angiogenesis in fibroblasts by p53 regulation of thrombospondm-1. Science 265, 1582. Folkman. J. (1996). Tumor angiogenesis and tissue factor. Nature Med. 2, 167-168 Folkman. J. (1989). What is the evidence that tumors are angiogenesis dependent?. J. Natl. Cancer Inst. 82, 4-6. Folkman, J. (1985). Angiogenesis and its inhibitors. In Important Advances in Oncology 1985, V. T. DeVita. S. Hellman. and S. Rosenberg, eds. (Philadelphia: J.B. Lippincott Company), pp. 42-62 Folkman, J., Haundenschild, C. C . and Zetter, B. R. ( 1979). Long-term culture of capillary endothelial cells. Proc. Natl. Acad. Sci. USA 76, 5217-5221. Gavneli, Y., Sherman. Y , and Ben-Sasson, S. A. (1992). Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J Cell Biol.. 119, 493-501. Good. D. J., Polvenni. P J.. Rast ejad, F., Le Beau, M. M.. Lemons, R. S., Frazier. W.A., and Bouck, N. P. (1990). A tumor suppressor-dependent inhibitor of angiogenesis is immunologically and functionally indistinguishable from a fragment of thrombospondin. Proc. Nat. Acad. Sci. USA. 87. 6624-6628. Grant. D. S., Tashiro, K.-l., Sequi-Real, B„ Yamada, Y., Martin. G. R., andKleinman, H. K. (1989). Two different lamimn domains mediate the differentiation of human endothelial cells into capillary-like structures in vitro. Cell 58, 933-943. Gross, J. L., Moscatelh, D., and Rifkin, D. B. (1983). Increased capillary endothelial cell protease activity in response to angiogenic stimuli in vitro. Proc. Natl. Acad. Sci. USA 80, 2623-2627. Gupta, S. K., Hassel, T., and Singh, J. P. (1995). A potent inhibitor of endothelial cell proliferation is generated by proteolytic cleavage of the chemokine platelet factor 4. Proc. Natl. Acad. Sci. USA 92, 7799-7803. Holmgren, L., O'Reilly, M. S., and Folkman, J. (1995). Dormancy of micrometastases: balanced proliferation and apoptosis in the presence of angiogenesis suppression. Nature Med. 1, 149-153. Homandberg, G. A.. Williams, J. E., Grant, D., B., S., and Eisenstein. R. (1985) Hepann-bmdmg fragments of fibronectin are potent inhibitors of endothelial cell growth. Am. J. Path. 120, 327-332. Hon. A., Sasada, R., Matsutani, E., Naito, K., Sakura, Y.. Fujita, T., and Kozai, Y. (1991). Suppression of solid tumor growth by lmmunoneutrahzing monoclonal antibody against human basic fibroblast growth factor. Cancer Res. 51, 6180-6184. Kandel, J., Bossy-Wetzel, E., Radvany, F., Klagsburn. M., Folkman, J., and Hanahan, D. (1991). Neovasculaπzation is associated with a switch to the export of bFGF in the multistep development of fibrosarcoma. Cell 66, 1095-1104. Kim, K. J., Li, B., Winer, J., Armanim, M., Gillett, N., Phillips, H. S., and Fenara, N. (1993). Inhibition of vascular endothelial growth factor-induced angiogenesis suppresses tumor growth in vivo. Nature 362, 841-844. Maione, T. E., Gray, G. S., Petro, J., Hunt, A. J., Donner, A. L., Bauer, S. I., Carson, H. F., and Sharpe, R. J. (1990). Inhibition of angiogenesis by recombinant human platelet factor-4 and related peptides. Science 247, 77-79. Millauer, B., Shawver, L. K., Plate, K. H., Risau, W., and Ullrich, A. (1994). Ghoblastoma growth inhibited in vivo by a dominant-negative Flk-1 mutant. Nature 367, 576-579. Muragaki, Y., Timmons, S., Griffith, C. M., Oh, S. P., Fadel, B., Quertemmous, T., and Olsen, B.- R. (1995). Mouse collδal is expressed in a tissue-specific manner as three alternative variants and is localized in basement membrane zones. Proc. Natl. Acad. Sci. USA 92, 8763-8767. Nelson, J., Allen. W. E., Scott, W. N., Bailie, J. R., Walker, B., and McFenan, N. V. (1995). Munne epidermal growth factor (EGF) fragment (33-42) inhibits both EGF- and lammin-dependent endothelial cell motihty and angiogenesis. Cancer Res. 55, 3772— 3776. Nguyen, M., Shing, Y., and Folkman, J. (1994). Quantitation of angiogenesis and antiangiogenesis in the chick embryo choπoallantoic membrane. Microvascular Res. 47, 31-40. O'Reilly, M. S., Holmgren, L., Chen, C. C, and Folkman, J. (1996). Angiostatin induces and sustains dormancy of human pnmary tumors in mice. Nature Med. 2, 689-692. O'Reilly, M. S., Holmgren, L., Sh g, Y., Chen, C, Rosenthal, R. A., Moses, M., Lane, W. S., Cao, Y., Sage, E. H., and Folkman, J. (1994). Angiostatin: A novel angiogenesis inhibitor that mediates the suppression of metastases by a Lewis lung carcinoma. Cell 79. 315-328. Obeso, J , Weber, J., and Auerbach. R. (1990). A hemangioendothehoma-deπved cell line: its use as a model for the study of endothelial cell biology. Lab. Invest. 63, 259-269. Oh, S. K., Kamagata, Y., Muragaki, Y., Timmons, S , Ooshima, A., and Olsen, B. R. (1994). Isolation and sequencing of cDNAs for proteins with multiple domains of Gly- Xaa-Yaa repeats identify a distinct family of collagenous proteins. Proc. Natl. Acad. Sci. USA 91, 4229-4233. Oh, S. K., Warman, M.L., Seldin, M.F., Cheng, S.D., Knoll, J. H. M., Timmons, S., and Olsen, B.R. (1994). Cloning of cDNA and Genomic DNA Encoding Human Type XVIII Collagen and Localization of the a l(SVIII) Collagen Gene to Mouse Chromosome 10 and Human Chromosome 21. Genomics 19, 494-499. Parangi, S., O'Reilly, M., Chnstofon, G., Holmgren, L., Grosfeld, J., Folkman, J., and Hanahan, D. (1996). Antiangiogenic therapy of transge c mice impairs de novo tumor growth. Proc. Natl. Acad. Sci. USA 93, 2002-2007. Rastinejad, F., Polvermi, P. J., and Bouck, N. P. (1989). Regulation of the activity of a new inhibitor of angiogenesis by a cancer suppressor gene. Cell 56, 345-355. Rehn, M., and Pihlajaniemi, T. (1994). al(XVIII), a collagen chain with frequent intenuptions in the collagenous sequence, a distinct tissue distribution, and homology with type XV collagen. Proc. Natl. Acad. Sci. USA 91, 4234-4238. Rehn, M., and Pihlajaniemi, T. (1995). Identification of three N-terminal ends of type XVIII collagen chains and tissue-specific differences in the expression of the conesponding transcnpts. J. Biol. Chem. 270, 4705-4711. Sage, E. H., Bassuk, J. A., Vost, J. C, Folkman. M. J., and Lane, T. F. (1995). Inhibition of endothelial cell proliferation by SPARC is mediated through a Ca (2+)-bιndmg EF— hand sequence. J. Cell Biochem. 57, 127-140. Sakamato, N., Iwahana, M., Tanaka, N. G., and Osaka, 8. (1991). Inhibition of angiogenesis and tumor growth by a synthetic laminin peptide, CDPGYIGSR-NH2. Cancer Res. 51, 903-906. Stπeter. R. M., Kunkel, S. L., Arenberg, D. A., Burdick, M. D., and Polveπni. P. J. (1995). Human mterferon-inducible protein 10 (IP-10), a member of the C-X-C chemokine family, is an inhibitor of angiogenesis. Biochem. Biophys. Res. Comm. 210, 51-57. Studier, W. F., Rosenberg, A. H., Dunn, J. J., and Dudendorf, J. W. (1990). Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 85, 60-89. Teicher, B. A., Holden, S. A., Ara, G., Sotomayor, E. A., and Dong, H. Z. (1994). Potentiation of cytotoxic cancer therapies by TNP-470 alone and with other antiangiogenic agents. Int. J Cancer 57, 1-6. Tolsma, S. S., Volpert, O. V , Good, D. J., Frazier, W. A., Polveπni, P. J., and Bouck, N. (1993). Peptides derived from two separate domains of the matrix protein thrombospondιn-1 have antiangiogenic activity. J. Cell Bιol.122. 497-511. Voest, E. E., Kenyon, B. M., O'Reilly, M. S., Truitt, G., D'Amato, R. J., and Folkman, J. (1995). Inhibition of angiogenesis in vivo by mterleukin 12. J. Natl. Cancer Inst. 87, 581 -586.

Claims: CLAIMS: 1. An isolated endostatin protein, wherein the protein has an amino acid sequence selected from the group consisting of SEQ ID NO 3 and SEQ ID NO:5 2. The endostatin protein of Claim 1 having an amino acid sequence of SEQ ID NO:3. 3. The endostatin protein of Claim 1 having an amino acid sequence of SEQ ID NO:5. 4. The endostatin protein of Claim 1 made by a process comprising, recombinantly producing the protein of Claim 1 in a recombinant expression system, and isolating the recombinantly produced protein in its un-refolded form. 5. The endostatin protein of Claim 4, wherein the endostatin protein has an ammo acid sequence of SEQ ID NO:3. 6. The endostatin protein of Claim 4, wherein the endostatin protein has an amino acid sequence of SEQ ID NO:5. 7. The endostatin protein of Claim 4, wherein the recombinant expression system is selected from the group consisting of bacterial expression systems, yeast expression systems and insect viral expression systems. 8. The endostatin protein of Claim 7, wherein the recombinant expression system is E. coli, Pichia, or baculovirus. 9. An isolated nucleic acid molecule having a sequence encoding an endostatin protein, wherein the nucleic acid sequence is selected from the group consisting of SEQ ID NO:4 and SEQ ID NO.6. 10. The isolated nucleic acid molecule of Claim 9 having the sequence of SEQ ID NO:4. 11. The isolated nucleic acid molecule of Claim 9 having the sequence of SEQ ID NO:6. 12 The isolated nucleic acid molecule of Claim 9. further comprising, a vector containing the nucleic acid sequence encoding endostatin protein, wherein the vector is capable of expressing an endostatin protein when present in a cell. 13. The isolated nucleic acid molecule of Claim 12. wherein the nucleic acid has a sequence of SEQ ID NOJ. 14 The isolated nucleic acid molecule of Claim 12. wherein the nucleic acid has a sequence of SEQ ID NO:6. 15 The endostatin protein of Claim 1, further comprising a pharmaceutically acceptable excipient. 16 The endostatin protem of Claim 15, wherein the endostatin protein has a sequence of SEQ ID NO:3. 17 The endostatin protein of Claim 15, wherein the endostatin protein has a sequence of SEQ ID NO:5. 18. The endostatin protein of Claim 15, wherein the pharmaceutically acceptable excipient provides a sustained release of the endostatin protein for a penod of at least 8 hours. 19. The endostatin protein of Claim 15, wherein the protein is administered to an individual for the treatment of an angiogenesis-related disease. 20. The endostatin protein of Claim 15, wherein the angiogenesis-related disease is an angiogenesis-dependent cancer.

Inventors: FOLKMAN M JUDAH, O'REILLY MICHAEL S, FOLKMAN, M., JUDAH, O'REILLY, MICHAEL, S.
Applicants: CHILDRENS MEDICAL CENTER, THE CHILDREN'S MEDICAL CENTER CORPORATION
Application year: 1999
Main classification: C12N15
IPC codes: A61K38, C07K14, C12N15