METHODS AND APPARATUS FOR ENHANCEMENT OF TRANSDERMAL TRANSPORT

Abstract: According to the present invention, a method for enhancing transdermal transport is disclosed. The method includes the steps of increasing a permeability of an area of a membrane with a permeabilizing device. The membrane may be, <u>inter alia</u>, biologic skin or synthetic skin. The permeabilizing device may be an ultrasound-producing device. A substance is transported into and through the area of the membrane. The substance may be a drug, a vaccine, or a component of interstitial fluid.

Description: METHODS AND APPARATUS FOR ENHANCEMENT OF TRANSDERMAL TRANSPORT BACKGROUND OF THE INVENTION 1. Field Of The Invention This invention relates to transdermal molecular transportation. More specifically, this invention relates to methods and apparatus for the regulation of skin permeabilization and analysis of analytes in extracted body fluid. 2. Description ofthe Related Art Drugs are routinely administered either orally or by injection. The effectiveness of most drugs relies on achieving a certain concentration in the bloodstream. Although some drugs have inherent side effects which cannot be eliminated in any dosage form, many drugs exhibit undesirable behaviors that are specifically related to a particular route of administration. For example, drugs may be degraded in the GI tract by the low gastric pH, local enzymes or interaction with food or drink within the stomach. The drug or disease itself may forestall or compromise drug absorption because of vomiting or diarrhea. If a drug entity survives its trip through the GI tract, it may face rapid metabolism to pharmacologically inactive forms by the liver, the first-pass effect. Sometimes the drug itself has inherent undesirable attributes such as a short half-life or a narrow therapeutic blood level range. Recently, efforts aimed at eliminating some of the problems of traditional dosage forms involve transdermal delivery of the drugs (TDD). Topical application has been used for a very long time, mostly in the treatment of localized skin diseases. Local treatments, however, only require that the drug permeate the outer layers of the skin to treat the diseased state, with little or no systemic accumulation. Transdermal delivery systems are designed for, inter alia, obtaining systemic blood levels, and topical drug application. For purposes of this application, the word "transdermal" is used as a generic term to describe the passage of substances into, out of, to, and through the skin. TDD offers several advantages over traditional delivery methods, including injections and oral delivery When compared to oral delivery, TDD avoids gastrointestinal drug metabolism, reduces first-pass effects, and provides sustained release of drugs for up to seven days, as reported by Elias in Percutaneous Absorption Mechanisms-Methodology-Drug Delivery, Bronaugh, R L Maibach, H I (Ed), pp 1-12, Marcel Dekker, New York, 1989 The transport of drugs to, into, out of, and through the skin is complex since many factors influence their permeation These include the skin structure and its properties, the penetrating molecule and its physical-chemical relationship to the skin and the delivery matrix, and the combination of the skin, the penetrant, and the delivery system as a whole Particularly, the skin is a complex structure There are at least four distinct layers of tissue the nonviable epidermis (stratum corneum, SC) the viable epidermis, the viable der is, the subcutaneous connective tissue Located within these layers are the skin's circulatory system, the arterial plexus, and appendages, including hair follicles, sebaceous glands, and sweat glands The circulatory system lies in the dermis and tissues below the dermis. The capillaries do not actually enter the epidermal tissue but come within 150 to 200 microns ofthe outer surface ofthe skin In comparison to injections, TDD can reduce or eliminate the associated pain and the possibility of infection Theoretically, the transdermal route of drug administration could be advantageous in the delivery of many therapeutic drugs, including proteins, because many drugs, including proteins, are susceptible to gastrointestinal degradation and exhibit poor gastrointestinal uptake Proteins, such as interferon, are cleared rapidly from the blood and need to be delivered at a sustained rate in order to maintain their blood concentration at a high value Transdermal devices are also easier to use than injections In spite of these advantages, very few drugs and no proteins or peptides are currently administered transdermally for clinical applications because of the low skin permeability to drugs This low permeability is attributed to the SC, the outermost skin layer which consists of flat, dead cells filled with keratin fibers (keratinocytes) surrounded by lipid bilayers The highly-ordered structure of the lipid bilayers confers an impermeable character to the SC (Flynn, G L , in Percutaneous Absorption Mechanisms-Methodology-Drug Delivery , Bronaugh, R L , Maibach, H I (Ed), pages 27-53, Marcel Dekker, New York 1989) Several methods have been proposed to enhance transdermal drug transport, including the use of chemical enhancers, i e , the use of chemicals to either modify the skin structure or to increase the drug concentration in a transdermal patch (Burnette, R R , in Developmental Issues and Research Initiatives, Hadgraft J , Guy, R H , Eds , Marcel Dekker 1989, pp 247-288, Junginger, et al in Drug Permeation Enhancement, Hsieh, D S , Eds , pp 59-90, Marcel Dekker, Inc New York 1994) and the use of applications of electric fields to create transient transport pathways (electroporation) or to increase the mobility of charged drugs through the skin (iontophoresis) (Prausnitz Proc Natl Acad Sci USA 90, 10504-10508 (1993), Walters, K A , in Transdermal Drug Delivery Developmental Issues and Research Initiatives, Ed Hadgraft J , Guy, R H , Marcel Dekker, 1989) Another approach that has been explored is the application of ultrasound Ultrasound has been shown to enhance transdermal transport of drugs across human skin, a phenomenon referred to as sonophoresis (Levy, J Clin Invest 1989, 83, 2974-2078, Kost and Langer in "Topical drug Bioavailability, Bioequivalence, and Penetration", pp 91 -103, Shah V P , Maibach H I , Eds (Plenum New York, 1993) For example, U S Patent No 4,309,989 to Fahim and U S Patent No 4,767,402 issued to Kost et al both describe the use of ultrasound in conjunction with transdermal drug delivery U S Patent No 4,309,989 discloses the topical application of a medication using ultrasound with a coupling agent such as oil Ultrasound at a frequency of at least 1000 kHz and a power of one to three W/cm 2 was used to create selective localized intracellular concentration of a zinc- containing compound for the treatment of herpes simplex virus U S Patent No 4,309,989, the disclosure of which is incorporated by reference in its entirety, discloses the use of ultrasound for enhancing and controlling transdermal permeation of a molecule, including drugs, antigens, vitamins, inorganic and organic compounds, and various combinations of these substances, through the skin and into the circulatory system Ultrasound having a frequency of about 20 kHz and having an intensity between about 0 and 3 W/cm 2 is used essentially to drive molecules through the skin and into the circulatory system Although a variety of ultrasound conditions have been used for sonophoresis, the most commonly used conditions correspond to therapeutic ultrasound (frequency in the range of between one MHz and three MHz, and intensity in the range of between above zero and two W/cm 2 ) (such as that described in the Kost et al patent) It is a common observation that the typical enhancement induced by therapeutic ultrasound is less than ten-fold In many cases, no enhancement of transdermal drug transport has been observed upon ultrasound application Accordingly, a better selection of ultrasound techniques is needed to induce a higher enhancement of transdermal drug transport by sonophoresis Application of low-frequency ultrasound (between about 20 and 200 kHz) can dramatically enhance transdermal transport of drugs, as well as the extraction and measurement of analyte, as described in PCT/US 96/ 12244 by Massachusetts Institute of Technology Transdermal transport enhancement induced by low-frequency ultrasound was found to be as much as 1000-fold higher than that induced by therapeutic ultrasound Another advantage of low-frequency sonophoresis as compared to therapeutic ultrasound is that the former can induce transdermal transport of drugs which do not passively permeate across the skin Ultrasound gels may be used as couplings in most medical applications of ultrasound energy Use of these gels may be messy and labor- intensive To overcome problems associated with applying ultrasound with gels and other coupling agents, patches containing the required components have been developed A patch adheres to a clean area of the skin, and drug molecules are continually absorbed through the skin into the bloodstream for systematic distribution These patches include a drug-containing layer provided near an ultrasonic oscillator Drug absorption is ensured by the action of the ultrasonic waves from the oscillator The amount of drug released may be controlled by varying the ultrasonic wave output from the oscillator, as described in U S Patent No 5,007,438 to Tachibana, et al , the disclosure of which is incorporated by reference in its entirety U S Patent No 4,821,740 to Tachibana et al discloses a kit for providing external medicines that includes a drug containing layer and an ultrasonic oscillator for releasing the drugs for uptake through the surface of the skin The transducer may be battery powered The application of the ultrasound causes the medication to move from the device to the skin and then the ultrasound energy may be varied to control the rate of administration through the skin U S Patent No 5,421 ,816 to Lipkovker describes ultrasonic energy that releases a stored drug and forcibly moves the drug through the skin of an organism and to the blood stream A housing includes a cavity defined by an assembly of ultrasonic transducers and separated from the skin by a polymeric membrane that stores the drug to be delivered The ultrasonic transducer assembly includes a flat, circular ultrasonic transducer that defines the top of a truncated cone and a polarity of transducer segments that define the walls ofthe cone The resonant frequency of the planar transducer is lower than the resonant frequency of the transducer segments The planar, flat, circular transducer generates a fixed frequency in the 5 kHz to 1 MHz range, and ultrasonic stimuli impulses for a predetermined period of time, such as 10-20 seconds Between the stimuli pulse periods, the transducer segments receive variable frequency ultrasonic pumping pulses The variable frequency ultrasonic pumping pulses lie in the 50 MHz to 300 MHz range The variable frequency ultrasonic pumping pulses are applied to opposing transducer segments The transducer segments create beams that impinge on the skin at an oblique angle to create a pulsating wave Further, the variable frequency ultrasonic pumping pulses are applied to opposing transducer segments in a rotating manner to create pulsating waves in the skin in a variety of directions The stimuli pulses cause the planar transducer to produce an ultrasonic wave that excites the local nerves in a way that trauma, such as heat and force, excites local nerves The variable frequency ultrasonic pumping pulses cause the transducer segments to produce ultrasonic waves in both the polymeric membrane and the skin The ultrasonic waves pump the drug to the polymeric membrane and, then, through skin openings into the underlying blood vessels Thus ultrasound energy may serve to enhance the flux of active permeate molecules through the skin and other biological membranes by providing an active energy source, in addition to passive diffusion, to push or pump molecules through pores and channels. In addition to there being a need to deliver drugs through the skin, there is a major medical need to extract analytes through the skin. For example, it is desirable for diabetics to measure blood glucose several times per day in order to optimize insulin treatment and thereby reduce the severe long-term complications of the disease. Currently, diabetics do this by pricking the highly vascularized fingertips with a lancet to perforate the skin, then milking the skin with manual pressure to produce a drop of blood, which is then assayed for glucose using a disposable diagnostic strip and a meter into which this strip fits. This method of glucose measurement has the major disadvantage that it is painful, so diabetics do not like to obtain a glucose measurement as often as is medically indicated. Therefore, many groups are working on non-invasive, and less invasive means to measure glucose, such as micro lancets that are very small in diameter, very sharp, and penetrate only to the interstitium (not to the blood vessels of the dermis). A small sample, from about 0.1 to two μl, of interstitial fluid is obtained through capillary forces for glucose measurements. Other groups have used a laser to breach the integrity of the stratum corneum and thereby make it possible for blood or interstitial fluid to diffuse out of such a hole or to be obtained through such a hole using pneumatic force (suction) or other techniques. An example of such a laser based sampling device is disclosed in US Patent No. 5,165,418 to Tankovich and WP1 ACC No: 94-167045/20 by Budnik (assigned to Venisect, Inc.). A problem with methods that penetrate the skin to obtain interstitial fluid is that interstitial fluid occurs in the body in a gel like form with little free fluid and, in fact, is even under negative pressure that limits the amount of free interstitial fluid that can be obtained. When a very small hole is made in the skin, penetrating to a depth such that interstitial fluid is available, it takes a great deal of mechanical O 00/35357 - 1 - force (milking, vacuum, or other force) to obtain the requisite quantity of blood, or interstitial fluid, used in a glucose meter Channeling of ultrasound geometrically is one way to apply ultrasound to a small area Channeling of ultrasound is disclosed in PCT Patent Application No PCT/US97/1 1559 entitled "Ultrasound Enhancement of Transdermal Transport" by Sontra L P et al , filed June 30, 1997, and incorporated by reference in its entirety The oscillation of a small element near or in contact with the surface of the skin is another way to apply ultrasound to a small area Large forces can be produced locally, resulting in cavitation, mechanical oscillations in the skin itself, and large localized shearing forces near the surface of the skin The element can also produce acoustic streaming, which refers to the large convective flows produced by ultrasound This appears to aid in obtaining a sample of blood or interstitial fluid without having to "milk" the puncture site Ultrasound transducers are known to rapidly heat under continuous operation, reaching temperatures that can cause skin damage Heat damage to the skin can be minimized by using a transducer that is located away from the skin to oscillate a small element near the skin In the case of analyte extraction, compounds present on the surface of and/or in the skin can contaminate the extracted sample The level of contamination increases as skin surface area increases Surface contamination can be minimized by minimizing the surface area of ultrasound application Thus, skin permeability can be increased locally, and transiently through the use ofthe methods and devices described herein, for either drug delivery or measurement of analyte Moreover, it has been disclosed that the application of ultrasound is only required once for multiple deliveries or extractions over an extended period of time rather than prior to each extraction or delivery That is, it has been shown that if ultrasound having a particular frequency and a particular intensity is applied, multiple analyte extractions or drug deliveries may be performed over an extended period of time For example, if ultrasound having a frequency of 20 kHz and an intensity of about 10 W/cm 2 is applied, the skin retains an increased permeability for a period of up to four hours This is described more particularly in United States Patent Application No 09/227,623 entitled "Sonophoretic Enhanced Transdermal Transport" by Mitragotri et al , filed on January 8, 1999, and in PCT Application No PCT/US99/00437 entitled "Sonophoretic Enhanced Transdermal Transport" by Sontra Medical et al , filed January 8, 1999 the disclosures of which are hereby incorporated by reference in their entireties Nevertheless, the amount (e g , duration, intensity, duty cycle etc ) of ultrasound necessary to achieve this permeability enhancement varies widely Several factors of the nature of skin must be considered For example, the type of skin which the substance is to pass through varies from species to species, varies according to age (e g , the skin of an infant has a greater permeability than that of an older adult), varies according to local composition, thickness and density, varies as a function of injury or exposure to agents such as organic solvents or surfactants, and varies as a function of some diseases, such as psoriasis, or abrasion Moreover, as discussed above, overexposure to ultrasound and cavitation can cause damage to the skin through heating and increased pressure Therefore, it is necessary to control the ultrasound application in order to enable clinically useful transdermal transport SUMMARY OF THE INVENTION Therefore, a need has arisen for a method and apparatus for regulation of skin permeabilization through a feedback system A need has arisen for a method and apparatus that provides controlled enhancement of transdermal transport A need has also arisen for a system and method for extraction and analysis of at least one analyte in a body fluid. A need has also arisen for a method and apparatus for sonophoretic drug delivery According to the present invention, a method for enhancing transdermal transport is disclosed The method includes the steps of increasing a permeability of an area of a membrane with a permeabilizing device Next, the permeability of the area of membrane is monitored A substance is transported into and through the area ofthe membrane According to one embodiment of the present invention, a method for enhancing permeability of an area of skin is disclosed The method comprises applying ultrasound to the area of skin While the ultrasound is being applied, electricity (e g , an ac current source or an ac voltage source) is applied to the area of skin While the electricity is being applied to the area of skin, a first electrical parameter of the area of skin is measured Based on the measured first electrical parameter, the ultrasound is controlled According to another embodiment, the present invention comprises an apparatus for enhancing the permeability of an area of skin The apparatus includes an ultrasound-producing device configured to apply ultrasound to the area of skin, an electrical source operable to apply electricity to the area of skin, a circuit to measure a first electrical parameter of the area of skin, and a controller responsive to the circuit and operable to control the ultrasound-producing device According to another embodiment, the present invention comprises a method for enhancing the permeability of an area of skin The method begins by creating a volume of fluid adjacent the area of skin The fluid has an initial concentration of a first substance Ultrasound is then applied to the area of skin While the ultrasound is being applied, changes in the concentration of the first substance are monitored Finally, the method controls the ultrasound based on the changes in the concentration ofthe substance According to another embodiment, the present invention comprises a method for enhancing the permeability of an area of skin The method begins by creating a volume of fluid adjacent the area of skin whose permeability is to be enhanced A reference value for an electrical parameter of the volume of fluid is then determined The method then applies ultrasound to the area of skin and monitors changes in the electrical parameter of the volume of fluid Finally, the ultrasound is controlled based on the changes in the electrical parameter of the volume of fluid According to another embodiment of the present invention, a method for regulating skin permeabilization is disclosed The method comprises coupling a first electrode in electrical contact with a first area of skin A second electrode is placed in electrical contact with a second area of skin The initial conductivity between these sites is measured, and then a skin permeabilizing method, such as ultrasound, is applied to the first area of skin The conductivity between the first area and second area is measured again Mathematical analysis or signal processing is performed on the conductivity information Next, parameters describing the kinetics of skin conductance are calculated Next, once the desired value of the parameters are reached, the skin permeabilizing step is terminated According to another embodiment, the present invention comprises an apparatus for enhancing permeability of an area of skin The apparatus includes a first electrode for coupling in electrical contact with a first area of skin, and a second electrode for placement in electrical contact with a second area of skin A skin permeabilizing device, such as an ultrasound-producing device, is provided to apply a skin permeabilizing treatment to the skin at the first area A means for measuring the conductivity between the first area and second area are provided A controller, for performing mathematical analysis or signal processing on the conductivity information, and for calculating the kinetics of skin conductance is provided The controller also controls the skin permeabilizing device According to another embodiment of the present invention, a method for extraction and analysis of at least one analyte in a body fluid is disclosed According to this method, first the permeability level of an area of skin is increased Next, a body fluid is extracted from the area of skin Then, the body fluid is collected Next, a determination is made as to the presence of at least one analyte in the body fluid The body fluid may be extracted by physical forces, chemical forces, biological forces, vacuum pressure, electrical forces, osmotic forces, diffusion forces, electro-magnetic forces, ultrasound forces, cavitation forces, mechanical forces, thermal forces, capillary forces, fluid circulation across the skin, electro- acoustic forces, magnetic forces, magneto-hydrodynamic forces, acoustic forces, convective dispersion, photo acoustic forces, by rinsing body fluid off skin, and any combination thereof The body fluid may be collected by a collection method including absorption, adsorption, phase separation, mechanical, electrical, chemically induced, and a combination thereof The presence of an analyte may be sensed by a sensing method including electrochemical, optical, acoustical, biological, enzymatic technology, and combinations thereof According to another embodiment of the present invention, a system for extraction and analysis of at least one analyte in a body fluid is disclosed The system comprises a transducer for increasing the permeability of an area of skin, an extraction device for extracting interstitial fluid from the area of skin, a collection device for collecting the extracted interstitial fluid, and a sensing device for sensing the presence of at least one analyte in the extracted interstitial fluid According to another embodiment of the present invention, a method for blood glucose determination is disclosed The method includes first increasing a permeability of an area of skin Next, interstitial fluid, or components thereof, is extracted from the area of skin In another embodiment, the interstitial fluid, or components thereof, diffuse through the skin, and are collected Next, the interstitial fluid is collected in a gel The gel may contain at least one glucose sensitive reagent that changes at least one characteristic of the gel, such as color, when glucose is present Finally, the change in the characteristic ofthe gel is monitored According to another embodiment of the present invention, a system for blood glucose determination is disclosed The system comprises a transducer for increasing the permeability of the skin, an extraction device for extracting interstitial fluid from the skin, a collection device for collecting the extracted interstitial fluid, a gel having at least one glucose sensitive reagent that changes a characteristic of the gel when glucose is present; and a monitoring device for monitoring a change in the characteristic of said gel According to another embodiment of the present invention, a drug delivery patch apparatus is disclosed The apparatus includes an ultrasound transducer for applying ultrasound to a membrane The membrane may include biological membranes, synthetic membranes, or a cell culture A biological membrane may include skin, mucosal and buccal membranes The apparatus further includes a power source coupled to the transducer The apparatus further includes drug molecules between the transducer and the membrane, and an attaching device that attaches the apparatus to the membrane According to another embodiment, the apparatus further includes drive electronics coupled to the transducer such that the drive electronics enables the transducer to apply ultrasound According to another embodiment, the apparatus further includes an interface coupled to the drive electronics The drug delivery patch apparatus may include the interface, drive electronics, power source, transducer, drug molecules, and attaching device contained within the patch for transdermal delivery through the membrane Alternatively, the transducer and the drug molecules as well as the attaching device may be contained in the patch The power source and interface may be connected to the patch with a connecting wire, or without a wire Alternatively, the drug delivery patch may include the power source, the transducer, the drug molecules and the attaching device within the patch The interface may be located elsewhere and communicates to the patch through hardwires, infrared, fiber optics, or telemetry According to another embodiment of the present invention, a method for transdermal vaccination by sonophoresis is disclosed According to the one embodiment, the method comprises the steps of enhancing the permeability of the skin by the application of ultrasound, providing a vaccine to the permeabilized skin, and delivering the vaccine to the skin cells, for example, Langerhans cells, dendric cells, and keratinocytes In another embodiment ofthe present invention, ultrasound is used to enhance the permeability of the skin In another embodiment of the present invention, the steps of increasing the permeability of the skin and providing a vaccine to the permeabilized skin occur simultaneously In another embodiment ofthe present invention, ultrasound is used to irritate or inflame an area of skin Next, a vaccine is provided to the irritated or inflamed skin This is more effective in inducing the immune response ofthe body BRIEF DESCRIPTION OF THE DRAWINGS The features and objects of the present invention, and the manner of attaining them is explained in detail in the following DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS of the invention when taken in conjunction with the accompanying drawings wherein „„, « , « PCT/ O 00/35357 - 13 - Fig. 1 depicts a schematic of an electrical model for skin, Fig. 2 depicts a flow chart of a method for controlled enhancement of transdermal delivery according to one embodiment ofthe present invention, Fig. 3 depicts a diagram of a circuit that enhances skin permeability and monitors enhancement of skin permeability according to one embodiment of the present invention, Fig. 4 depicts a permeability monitoring circuit according to another embodiment ofthe present invention, Fig. 5 depicts a permeability monitoring circuit according to one embodiment ofthe present invention, Fig. 6 depicts a flow chart of a method for controlled enhancement of transdermal delivery according to one embodiment ofthe present invention, Fig. 7 depicts a flow chart of a method for controlled enhancement of transdermal delivery according to one embodiment ofthe present invention, Fig. 8 depicts a flow chart of a method for controlled enhancement of transdermal delivery according to one embodiment ofthe present invention, Fig. 9 depicts the time variation of the skin conductance while being exposed to ultrasound according to an example, Fig. 10 shows a relationship between the inflection time and the time to pain on various volunteers according to an example, Fig. 11 depicts a flowchart of a method of determining when to terminate the application of ultrasound, Fig. 12 depicts example graphs ofthe method of Fig. 11 , Fig. 13 depicts a flowchart of a method for extraction and analysis of at least one analyte in a body fluid according to one embodiment of the present invention, Fig. 14 depicts a drawing of a tensioner according to one embodiment ofthe present invention, Fig. 15 depicts a flowchart of a method of determination of blood glucose according to one embodiment ofthe present invention, Fig. 16 illustrates a drug delivery patch apparatus in accordance with one embodiment ofthe present invention; Fig. 17 illustrates a cross-sectional view of a transducer in accordance with one embodiment ofthe present invention; Fig. 18 illustrates a drug delivery patch apparatus having a feedback mechanism in accordance with one embodiment ofthe present invention, and Fig. 19 depicts a flowchart ofthe method for transdermal vaccination by sonophoresis according to one embodiment ofthe present invention. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS As used herein, the terms skin permeabilizing method or device includes the application of ultrasound, chemicals, electroporation, mechanical, disrupting devices, tape stripping, and laser, and devices for the application of the same. In addition, the term skin includes membranes, such as biologic and synthetic skin. Ultrasound is generally defined as sound at a frequency of greater than about 20 kHz. Therapeutic ultrasound is typically between 20 kHz and 5 MHz. Sonophoresis is defined as the application of ultrasound to the skin resulting in enhanced transdermal transport of molecules. Low frequency sonophoresis or ultrasound is defined as sonophoresis or ultrasound at a frequency that is less than 2.5 MHz, more typically less than 1 MHz, more preferably in the range of 20 to 100 kHz. Near ultrasound is typically about 10 kHz to 20 kHz. It should be understood that in addition to ultrasound, near ultrasound may also be used in the embodiments ofthe present invention. 1. Enhancement and Regulation of Skin Permeability The use of ultrasound to facilitate transdermal transport is known. The mechanism by which ultrasound is used to facilitate transdermal transport has differed. In the context of transdermal delivery systems, ultrasound was initially used as a driving force that essentially pushed drugs through the skin and into the circulatory system. Ultrasound is also used to increase the permeability of the skin. That is, application of ultrasound having a particular frequency will disorganize the lipid bilayer in the skin and thus increase the permeability of the skin In this context, either drugs can be delivered through the skin to the body or analyte or analytes can be extracted through the skin from the body A driving force of some type is still required, but the required intensity ofthe driving force is decreased For example, a concentration gradient is generally sufficient driving force for transdermal transport through skin whose permeability has been enhanced using ultrasound Regardless of which mechanism is used, the ultrasound still needs to be controlled That is, overexposure to ultrasound may cause skin damage from increased heat, increased pressure and other factors Therefore according to various embodiments of the present invention, a method and an apparatus for controlled enhancement of skin permeability are disclosed The method and apparatus, according to the present invention, focus on the use of electrical parameters of the skin as a proxy for skin permeability The skin can be modeled using an R-C circuit similar to that shown in Fig. 1. The "skin circuit," shown in Fig. 1, consists of a resistor Ri in parallel with a capacitor C, both of which are in series with a resistor R 2 . For normal, intact skin, of an area of about 1.7 cm 2 , the value for Ri is about 100 kΩ, the value for C is about 13 μF and the value for R 2 is about 2kΩ Of course, these values will vary from person to person depending on skin type and condition By its nature, the behavior (i e , the frequency response) of the "skin circuit" changes in response to excitations having different frequencies For example, under normal conditions, the impedance of this circuit will decline sharply as frequency increases, for example, from 10 Hz to 1 kHz That is, at low frequencies, the capacitive component to the impedance of the parallel combination of Ri and C is significant and therefore the overall impedance of the circuit is high At higher frequencies, however, the capacitive component to the impedance of the parallel combination decreases and, therefore, the overall impedance of the "skin circuit" declines Various electrical parameters of the skin (e_g_, impedance, conductance, inductance and capacitance) have values that correlate with skin permeability For example, in the circuit of Fig. 1, the value of Ri significantly decreases as the skin becomes permeable For example, Ri may drop to a value around 5 kΩ for a skin area of about 1 7 cm Therefore, the frequency response of the overall skin circuit becomes much flatter as frequency increases That is, the difference between the impedance of the circuit at 10 Hz and 1 kHz would not be nearly as significant as at 10 Hz alone Thus, the methods and apparatus of the present invention measure one or more electrical parameters of an area of skin that is being exposed to ultrasound and then adjust the source of ultrasound based on the measured parameters According to one embodiment of the present invention, a method for controlled enhancement of skin permeability is disclosed, and will be explained in conjunction with Fig. 2 Typically, when a skin permeabilizing device, such as an ultrasonic device, is used to enhance transdermal transport properties, the skin permeabilizing device is applied to a small patch of skin In step 202, a baseline measurement for some electrical parameter is determined for the patch of skin to which the skin permeabilizing will be applied to determine baseline parameters In one embodiment, a baseline impedance is measured for the patch of skin to which the skin permeabilization device is to be applied In other embodiments, a baseline conductance, a baseline capacitance, a baseline inductance, or a baseline capacitance may be measured The baseline measurement may be made by using two or more electrodes As is shown in greater detail in Fig. 3, an electrode, such as source electrode 310, is coupled to the patch of skin to which ultrasound is to be applied Source electrode 310 does not have to make direct contact with the skin Rather, it may be electrically coupled to the skin through the medium that is being used to transmit ultrasound A second or counter electrode, such as conductive band 312, may be positioned on a second area of skin that the skin permeabilizing device will not be applied to This second area of skin can be adjacent to the patch of skin to which the skin permeabilizing device will be applied, or it can be distant from that patch of skin In one embodiment, the ultrasonic transducer and horn that apply the ultrasound double as the source electrode through which electrical parameters of the patch of skin may be measured, and is coupled to the skin through a conductive solution, such as saline, used as an ultrasound medium In another embodiment, a separate electrode may be affixed to the area of skin that ultrasound will be applied to and is used as the source electrode In still another embodiment, the housing of the device used to apply ultrasound to the area of skin may be used as the source electrode The electrode can be made of any suitable conducting material including, for example, metals and conducting polymers In order to achieve an accurate electrical reading, the counter electrode should make sufficient contact with the skin This can be achieved in a number of ways In one embodiment, the counter electrode is applied directly to the epidermis of the skin That is, the counter electrode is applied to an area of skin from which the stratum corneum has been removed The stratum corneum may be removed in a number of ways According to one embodiment, the stratum corneum is removed by tape stripping In one embodiment, sufficient electrical contact between the skin and the counter electrode is created by using a counter electrode having a large surface area More specifically, a conductive polymeric path or metallic foil patch having an area much larger than the skin area exposed to ultrasound is used The large area of the counter electrode in this embodiment decreases its impedance and allows accurate measurements of the electrical parameter of the area of skin exposed to ultrasound In one specific embodiment a conductive band is wrapped around the subject's arm and used as the counter electrode In another embodiment, the counter electrode may be placed in a handle ofthe skin permeabilizing device, to which a subject grasps during operation In another embodiment, the counter electrode surrounds the skin permeabilizing device When the two electrodes are properly positioned, the baseline measurement may be made by applying an electrical signal to the patch of skin through the electrodes The electrical signal supplied preferably has a sufficient intensity so that the electrical parameter of the skin can be measured, but a suitably low intensity so that the electrical signal does not cause damage to the skin or any significant electrophoresis effect for the substance being delivered In one embodiment, a 10 Hz AC source may be used to create a voltage differential between the source electrode and the counter electrode In one embodiment, in order to avoid a risk of permanent damage to the skin, the voltage supplied does not exceed 500 mV, and, preferably, does not exceed 100 mV In another embodiment, an AC current source is used The current source may also be similarly limited The baseline measurement is made after the source has been applied using appropriate circuitry In one embodiment, a resistive sensor is used to measure the impedance of the patch of skin at 10 Hz In another embodiment, a 1 kHz source is used Sources of other frequencies are also possible Experiments were performed on human volunteers to ensure that the above described electrode placement would provide accurate measurements A first glass chamber (~1 5 cm 2 in area) was placed on the forearm and was secured in place with an elastic strap This first chamber was filled with 2 ml of 1% sodium lauryl sulfate (SLS) in saline An ultrasound horn was place within the chamber and used to apply ultrasound to the skin Additionally, an electrode used to measure electrical parameters was incorporated into the horn A second small chamber (~1 5 cm ) was placed on the subject's arm in order to measure skin conductivity This is referred to as the reference chamber The skin under the reference chamber was tape-stripped using scotch tape to remove the stratum corneum This process involved placing a piece of scotch tape (1 5 cm wide and 3 cm long) on the subject's arm and removing it This procedure is repeated -25 times in order to remove the stratum corneum from the designated area An electrode was then placed on the skin under the chamber Another electrode is placed on the subject's arm This electrode consisted of a large piece of aluminum foil placed on intact skin Ultrasound (27 kHz, ~10μm tip displacement, pulsed 5 sec on/ 5 sec off) was applied to the first chamber The conductance of the skin exposed to ultrasound was measured with both counter electrodes (tape stripped and intact) The measured conductances were similar thus proving that a large counter electrode placed over intact skin can be successfully used to measure skin conductance during sonophoresis Referring again to Fig. 2, in step 204, the skin permeabilizing device, such as an ultrasound providing device, is applied to the patch of skin. Although the exact ultrasound parameters are not the subject of this invention, according to one embodiment using an ultrasonic device as a skin permeabilizing device, ultrasound having a frequency of about 20 kHz, and an intensity of about 10 W/cm 2 may be used to enhance the permeability of the patch of skin to be used for transdermal transport. After the skin permeabilizing device has been turned on, in step 206 the permeability of the patch of skin is monitored. More specifically, and as discussed above, electrical parameters of the patch of skin are used as a proxy for skin permeability. That is, what is actually being monitored is the electrical parameter for which a baseline measurement was made in step 202. The monitoring measurements are made using the same electrode set up that was used to make the baseline measurement. In step 208, the skin permeabilizing device is controlled based on the monitoring measurements made in step 206. In one embodiment, the monitoring measurements are fed back to a microcontroller that is used to control the skin permeabilizing device. When ultrasound is used, the permeability enhancement obtained by supplying ultrasound is limited. That is, once a certain permeability is reached, the further application of ultrasound will not further enhance skin permeability. Overexposure to ultrasound, or cavitation caused thereby, may result in damage to the skin from localized pressure, temperature increases, and shear stresses. Therefore, in one embodiment, when the parameter being monitored reaches is predetermined value, the ultrasound-producing device is turned off. If the parameter being monitored has not reached the predetermined value, the measurement is repeated until the predetermined value is reached. The predetermined value may depend upon a number of factors including, inter alia, the skin characteristics of the individual, the drug to be delivered or the analyte or analytes to be extracted (because of varying molecule sizes), and the frequency of the excitation source. As is apparent to one of ordinary skill in the art, a specific correlation between the electrical parameter being used and skin permeability may be determined by conducting experiments and using experimental data The predetermined value may then be determined on a subject- by-subject basis, taking into account all appropriate factors and the empirical data According to another embodiment, the intensity of the skin permeabilizing device may be gradually scaled back as the point of maximum permeability enhancement is approached In one embodiment, as the parameter being monitored reaches 50% of the predetermined value, either the intensity or the duty cycle may be reduced by a predetermined amount, such as 50% This is done so that the predetermined value is not "overshot," thereby increasing the risk of skin damage Additional controls are possible For example, in another embodiment, the intensity may be scaled back when the parameter being monitored reaches 25%, 50% and 75% ofthe predetermined value According to another embodiment, permeability enhancement control may be accomplished using two electrical sources having different frequencies This method relies on the observation, discussed above, that as the skin becomes more permeable, the frequency response of the skin becomes flatter In this embodiment, the initial step 202 of measuring a baseline for the parameter is unnecessary because the ultrasound control is based on a differential between the parameter value at two different frequencies of excitation Nevertheless, a baseline measurement may still be desirable in order to determine the range of values to expect In this embodiment, the electrode arrangement may be the same as that described above And, step 204 of beginning ultrasound application is also the same as recited above Thus, the details of these steps will not be reiterated After the skin permeabilizing has begun, in step 206, skin permeability is monitored In this embodiment, skin permeability is also monitored using an electrical parameter measured from the skin as a proxy This embodiment differs from the first embodiment in that the electrical parameter is measured at two frequencies In one embodiment, the impedance of the skin is measured at frequencies of 10 Hz and 1 kHz These measurements are then used to control the skin permeabilizing device According to this embodiment, in step 208 the parameter measurement at a first frequency is compared with the parameter measurement at a second frequency to determine whether the two measurements are within a predetermined differential If the two values are within a predetermined differential, it provides an indication that the frequency response of the skin has flattened and, therefore, is an indication that the skin has reached an enhanced level of permeability At this point, the skin permeabilizing device is turned off In one particular embodiment, an impedance of the skin is measured at 10 Hz and at 1 kHz And, if the two impedance measurements are within 20% of each other, the skin permeabilizing device may be turned off The rate of change in the parameter measurements may also be used to determine a point at which the skin permeabilizing device is scaled back or discontinued The rate of change of one, or both, or the parameters may be used In another embodiment, the rate of change of the difference between the two parameters may also be used As the rate of change reaches a predetermined value, the intensity of the skin permeabilizing device may be gradually scaled back or discontinued, in a manner similar to that discussed above As discussed above, the predetermined differential value may depend upon a number of factors, including, inter alia, the skin characteristics of the individual, the drug to be delivered or the analyte to be extracted (because of varying molecule sizes), and the frequencies of the exciting sources Therefore, the predetermined differential is determined on a subject-by-subject basis taking into account all appropriate factors Empirical data may be used to determine a precise value for the predetermined differential. In a modification of this embodiment, the intensity of the skin permeabilizing device may be gradually scaled back as the point of maximum permeability enhancement is approached For example, as the differential between the two parameter measurements approaches 50% of the predetermined differential value, either the intensity or the duty cycle may be reduced by a predetermined amount, such as 50% Additional controls are possible For example, in another embodiment, the intensity is scaled back when the differential between the two parameters being monitored reaches 25%, 50% and 75% of the predetermined differential value In vitro experiments were performed to assess the above two source method Pig skin was mounted on diffusion cells Skin was mounted on the diffusion cell and was exposed to ultrasound using 1% Sodium Lauryl Sulfate and saline solution as a coupling medium Skin conductance was measured by placing two electrodes across the skin The impedances were measured at two frequencies 10 Hz and 1 kHz The impedances measured at the frequencies differed by about 25 fold prior to application of ultrasound when the skin was not permeable Upon sonication, the difference between the impedances at two frequencies decreased The decrease in the differential impedance increased with time When the skin was highly permeable, the impedances at two frequencies differed only by -20% Thus the difference between the impedances measured at two frequencies may be used to determine the level of permeabilization and stop sonication The methods described above use a single electrical parameter to control the ultrasound-producing device Nevertheless, control of the ultrasound- producing device may also be based on two or more electrical parameters According to another embodiment of the present invention, an apparatus for controlled enhancement of transdermal transport 300 is described in conjunction with Fig. 3 Apparatus 300 uses an ultrasound-producing device as the skin permeabilizing device, it should be noted that other devices for increasing the skin permeability may be used in place of the ultrasound-producing device For example, the permeability of the skin may be increased through the application of electric fields, chemicals, mechanical forces, needles, and magnetic forces Apparatus 300 includes ultrasound transducer/horn combination 302, source 304, bandpass filter 306, permeability monitoring circuit 308, source electrode 310, return electrode 312, and microcontroller 314 Permeability monitoring circuit 308 comprises current sensor 315, amplifier 316, A/D converter 318, and resistor 320 Ultrasound transducer/horn combination 302 is used to apply ultrasound to the area of skin 322 Transducer 302 may be any known ultrasound transducer, such as a piezoelectric transducer, a ceramic transducer, or polymer block transducer The horn can have any known configuration In one embodiment the horn is made of a conductive metal As described above, while the ultrasound is being supplied to the area of skin, it is important to monitor the skin permeability and control the ultrasound application so that the skin will not be overexposed to ultrasound Apparatus 300 may include the electrical control circuitry elements described above in order to accomplish this monitoring and control Specifically, source 304 and bandpass filter 306 are provided to drive the electrical control circuitry That is, in order to obtain the electrical parameter measurements used for controlling source 304, a small signal is passed through the area of skin In one embodiment of the present invention, source 304 provides a 10 Hz AC square wave voltage that is used to monitor the permeability ofthe area of skin in apparatus 300 Bandpass filter 306 is provided to convert the square wave into a sinusoid Source electrode 310 and return electrode 312 provide an electrical path through which electrical parameters of the area of skin 322 can be measured Source electrode 310 may be incorporated into transducer/horn combination 302, and is preferably formed of any suitable conductive material In one embodiment, the ultrasound horn is metal and is used as the source electrode Return electrode 312 is a conductive band and is preferably formed from a conductive polymeric path or a metallic foil Permeability monitoring circuit 308 comprises circuitry designed to measure an electrical parameter of the skin as a proxy for the permeability of the skin More specifically, according to one embodiment of the present invention, permeability monitoring circuit 308 comprises circuitry designed to measure the current flow through the area of skin 322 and to convert that measurement in to a form suitable for use by microcontroller 314 Permeability monitoring circuit 308 comprises current sensor 315 that is operable to measure the impedance of area of skin 322 Current sensor 315 may be any sensor that may be used to measure current, and, in one embodiment, current sensor 315 is a 1 kΩ current sense resistor where the output voltage generated is 1000 times the current flowing through the skin The output of current sensor 315 is an analog signal that should be digitized before it may be used by microcontroller 315 Amplifier 316 and resistor 320 serve to amplify the output voltage of current sensor 315 so that it may be digitized by A/D converter 318 A/D converter 318 may be any suitable A/D converter The signal from A/D converter 316 may then be provided to microcontroller 314 Microcontroller 314 may be any suitable microcontroller Microcontroller 314 is programmed to control transducer driver circuit 324 as described above In one embodiment, microcontroller 314 determines whether the signal from permeability monitoring circuit 308 is greater than some predetermined value If so, microcontroller 314 may turn off the ultrasound by, for example, shutting off the D C supply for transducer driver circuit 324 Microcontroller 314 may also be configured to provide other controls, such as altering the duty cycle of transducer driver circuit 324 through the phase lock loop circuit According to one embodiment of the present invention, additional controls and a user interface may be provided Fluids controller 330 controls the pumps and fluids for the system Pump 332 may be provided to provide a seal between transducer 302 and the surface of skin 322 Pump 334, in conjunction with valve 336, may be used to fill and evacuate the chamber of transducer 302 The coupling fluid used in transducer 302 may be provided in cartridge 338 Other devices and methods for providing coupling fluid may also be used A user interface may also be provided User interface 340 includes low battery sensor 342, which may include a comparator Switch 344 may be provided to turn on or off the ultrasound-producing device Input 346 may be provided to allow a user to adjust the ultrasound intensity The ultrasound level may be provided in display 350 The permeability level of the skin may be provided in display 352 Indicators 354 and 356 may be provided to alert the user of the operation of the ultrasound, as well as a when there is a low battery Additional controls and displays may be provided, as required, to prevent a user from applying ultrasound of a harmful intensity or duration, or to prevent ultrasound from being applied before the system is ready (i_e_, before coupling fluid is provided for transducer 302, etc ) The circuitry described above may be replaced with other elements if the electrical parameter measurements are accomplished in a different way More specifically, the circuitry shown in Figs. 4 or 5 could be used in place of source 304, bandpass filter 306, and permeability monitoring circuit 308 if the control methodology using sources at two frequencies was to be used Fig. 4 schematically depicts one embodiment of a circuit useful for implementing dual frequency control of skin permeability The circuit comprises sources F t and F 2 that supply two distinct AC signals to the area of skin to which ultrasound is being applied In one embodiment, sources F] and F comprise a 10 Hz and a 1kHz current source respectively These sources are alternately applied to the area of skin through a microprocessor controlled switch In the embodiment shown in Fig. 3, microcontroller 314 would control the switch so that sources Fi and F 2 alternately excite the skin After excitation by one of the sources, the impedance of the skin is measured by measuring the voltage Vi That is, Vj is transmitted to a microprocessor (e g , microcontroller 314 in Fig. 3) through gain circuit 402, diode 404, capacitor Ci, and output resistors Roi and R 02 The combination of diode 404 and capacitor Ci comprises an AC to DC converter suitable for input to an A/D converter to transform the analog signal from gain circuit 402 to a digital signal suitable for use by a microprocessor Output resistors Roi and R 02 provide impedance matching and filtering for the microprocessor, respectively In operation, the circuit of Fig. 4 in conjunction with a suitably programmed microcontroller alternately applies a 10 Hz and a 1kHz AC source to the skin The circuit, in conjunction with the microprocessor, measures the impedance of the skin at both frequencies The microcontroller makes suitable adjustments to the ultrasound-producing device based on the differential between the impedance ofthe skin at 10 Hz and the impedance of the skin at 1 kHz Fig. 5 schematically depicts yet another embodiment of permeability monitoring circuit for use with multiple frequency excitation In the circuit of Fig. 5, sources Fi and F 2 are applied simultaneously through adder circuit 502 to the area of skin to which ultrasound is being applied The output signal from the skin is then fed to two bandpass filters 504 and 506 Elements C], C 2 and Ri of bandpass filter 504 are preferably chosen to create a pass band centered around the frequency of source Fi Elements C 3 , C 4 and R of bandpass filter 506 are preferably chosen to create a pass band centered around the frequency of source F 2 The output signals from bandpass filters 504 and 506 are then subtracted in comparator circuit 508 to create a differential signal for the microprocessor A suitably configured microprocessor then uses this differential signal to make suitable adjustments to the ultrasound-producing device In another embodiment of the present invention, a method for controlled enhancement of skin permeability by coupling fluid monitoring is disclosed When a skin permeabilizing device, such as an ultrasound-producing device, is applied to the skin, it is applied through some coupling fluid, which may be a liquid, gel, or solid, to facilitate transfer of the energy in the high frequency sound waves to the skin As the skin becomes more permeable, suitably-sized molecules and ions in the coupling fluid begin to pass into and out of the skin The method according to this embodiment takes advantage of the enhanced skin permeability that is the desired end point of this invention in order to control the skin permeabilizing device This method will be explained in conjunction with the flow chart of Fig. 6 In step 602 an initial concentration of a known substance is determined for the coupling medium In practice, the coupling medium may have a known initial concentration of a known substance That is, step 602 will not require any additional measuring The known substance can be any substance (molecular or ionic) as long as its concentration in the coupling medium is known If, however, the substance is going to be passed into the body, the substance should be one that is not harmful to the body The term benign is used herein to describe such a substance Glucose and calcium are examples of substances that may be used in this embodiment In step 604, the skin permeabilizing device is applied to the patch of skin In one embodiment, an ultrasound-producing device is used as the skin permeabilizing device Although the exact parameters of ultrasound are not the subject of this invention, according to one embodiment, ultrasound having a frequency of about 20 kHz, and an intensity of about 10 W/cm 2 is used to enhance the permeability ofthe patch of skin to be used for transdermal transport After the skin permeabilizing device has been turned on, in step 606 the permeability of the patch of skin is monitored According to this embodiment, permeability monitoring is accomplished by monitoring changes in the concentration of the known substance in the coupling medium That is, as the area of skin is subjected to the skin permeabilizing device it will become permeable As the area of skin becomes permeable, molecules and ions begin to pass into the coupling medium from inside the body and from the coupling medium into the body depending on the concentration gradient of the substance between the body and the coupling medium This concentration monitoring may be done in real time using an on-line sensor specifically programmed to detect and measure the concentration of the known substance In one embodiment, glucose is used as the known substance The concentration of glucose is usually greater inside the body than in the coupling medium unless the concentration in the coupling medium is artificially increased Thus, when the skin becomes permeable, glucose molecules will begin to pass into the coupling medium In step 606, changes in the concentration of glucose in the coupling medium are monitored to determine when the skin becomes permeable In another embodiment, mannitol is used as the known substance Mannitol is a benign substance as that term is used in the context of this application The concentration of mannitol in the coupling medium is adjusted so that it is greater than the concentration of mannitol in the body When the skin becomes permeable, mannitol molecules with begin to pass from the coupling medium into the body, decreasing the concentration of mannitol in the coupling medium In step 606, the decrease in the concentration of mannitol in the coupling medium is monitored to determine when the skin becomes permeable In step 608, the skin permeabilizing device is controlled based on the concentration measurements made in step 606 In one embodiment, the concentration measurements from the chemical analyzer are fed back to a microcontroller that is used to control the skin permeabilizing device According to another embodiment, when the concentration of the substance being monitored reaches a predetermined value, the skin permeabilizing device is turned off If the concentration of the substance being monitored has not reached the predetermined value, the measurement is repeated until the predetermined value is reached The predetermined value depends upon a number of factors including, inter alia, the skin characteristics of the individual, the known substance, and the frequency of the excitation source As is apparent to one of ordinary skill in the art, a specific correlation between the change in concentration of the known substance being used and skin permeability can be determined by conducting experiments and using experimental data The predetermined value is then determined on a subject-by- subject basis taking into account all appropriate factors as well as any empirical data According to another embodiment, the intensity of the skin permeabilizing device may be gradually scaled back as the point of maximum permeability enhancement is approached In one embodiment, where an ultrasound- producing device is used, as the concentration of the substance being monitored approaches 50% of the predetermined value, either the intensity or the duty cycle of the ultrasound may be reduced by a predetermined amount, such as 50% This is done so that the predetermined value is not "overshot" thereby increasing the risk of skin damage Additional controls are possible For example, in another embodiment, the intensity may be scaled back when the concentration of the substance being monitored reaches 25%, 50% and 75% ofthe predetermined value The rate of change in the concentration of the substance may also be used to determine a point at which the skin permeabilizing device is scaled back or discontinued As the rate of change in the concentration reaches a predetermined value, the intensity of the skin permeabilizing device may be gradually scaled back or discontinued, in a manner similar to that discussed above In another embodiment, skin permeability can be monitored by detecting an electrical parameter of the coupling fluid More specifically, as skin permeability increases, ions may pass into and out of the coupling medium As ion concentration in the coupling medium increases or decreases, the electrical characteristics of the coupling medium change Therefore, the electrical characteristics of the coupling medium can be used to monitor skin permeability using a methodology that is a hybrid of that shown in Figs. 2 and 6, and is set forth in Fig. 7 In an initial step 702, a reference value for an electrical parameter is determined for the coupling medium In practice, because the coupling medium has a known ionic composition, its electrical parameters should be known In one embodiment, the coupling fluid has a known concentration of calcium ions Thus, this step should not require an actual measurement In another embodiment, the electrical parameter determined is conductivity, and in step 702, the conductivity of the coupling medium is determined After the reference value for the electrical parameter is determined, in step 704 the skin permeabilizing device is turned on Then in step 706 skin permeability is determined by monitoring changes in the electrical parameter of the coupling medium This monitoring may be accomplished using a simple meter As the skin becomes permeable, depending upon the composition of the coupling medium, ions will pass into or out of the coupling medium and either increase or decrease the electrical parameter of the coupling medium In one embodiment, the coupling medium has a known concentration of calcium ions that is lower than the concentration of calcium ions in the body Therefore, as the skin becomes more permeable, calcium ions begin to pass from the body into the coupling medium In step 708, the skin permeabilizing device is controlled based on the monitoring measurements In one embodiment, the monitoring measurements are fed back to a microcontroller that is used to control the skin permeabilizing device In one embodiment, when the electrical parameter being monitored reaches is predetermined value, the skin permeabilizing device is turned off If the parameter being monitored has not reached the predetermined value, the measurement is repeated until the predetermined value is reached The rate of change in the parameter being monitored may also be used to determine a point at which the skin permeabilizing device is scaled back or discontinued. As the rate of change reaches a predetermined value, the intensity of the skin permeabilizing device may be gradually scaled back or discontinued, in a manner similar to that discussed above. The predetermined value depends upon a number of factors including, inter alia, the composition of the coupling medium, the surface area ofthe patch of skin to which the skin permeabilizing device is applied, and the concentration of the particular ion being used in the body. The predetermined value is determined on a subject-by- subject basis taking into account all appropriate factors and the empirical data. According to another embodiment, the intensity of the skin permeabilizing device may be gradually scaled back as the point of maximum permeability enhancement is approached. In one embodiment, where ultrasound is used, as the parameter being monitored reaches 50% of the predetermined value, either the intensity or the duty cycle is reduced by a predetermined amount, such as 50%. This is done so that the predetermined value is not "overshot" thereby increasing the risk of skin damage. Additional controls are possible. For example, in another embodiment, the intensity may be scaled back when the parameter being monitored reaches 25%, 50% and 75% ofthe predetermined value. According to another embodiment of the present invention, an apparatus and method for regulating the degree of skin permeabilization through a feedback system is provided. This apparatus and method may be similar to what has been described above, with the addition of further regulation of the degree of skin permeabilization. In this embodiment, however, the application of the skin permeabilizing device is terminated when desired values of parameters describing skin conductance are achieved. As the discussion proceeds with regard to Fig. 8, it should be noted that the descriptions above may be relevant to this description. Referring to Fig. 8, a flowchart of the method is provided. In step 802, a first, or source, electrode is coupled in electrical contact with a first area of skin where permeabilization is required. As discussed above, the source electrode does not have to make direct contact with the skin. Rather, it may be electrically coupled to the skin through the medium that is being used to transmit ultrasound. In one embodiment, where an ultrasound-producing device is used as the skin permeabilizing device, the ultrasonic transducer and horn that will be used to apply the ultrasound doubles as the source electrode through which electrical parameters of the first area of skin may be measured and is coupled to the skin through a saline solution used as an ultrasound medium In another embodiment, a separate electrode is affixed to the first area of skin and is used as the source electrode In still another embodiment, the housing of the device used to apply ultrasound to the first area of skin is used as the source electrode The source electrode can be made of any suitable conducting material including, for example, metals and conducting polymers Next, in step 804, a second, or counter, electrode is coupled in electrical contact with a second area of skin at another chosen location This second area of skin can be adjacent to the first area of skin, or it can be distant from the first area of skin The counter electrode can be made of any suitable conducting material including, for example, metals and conducting polymers In order to get an accurate electrical reading, the counter electrode should make sufficient contact with the skin This can be achieved in a number of ways In one embodiment, the counter electrode is applied directly to the epidermis of the skin That is, the counter electrode is applied to an area of skin from which the stratum corneum has been removed The stratum corneum may be removed in a number of ways According to one embodiment, the stratum corneum is removed by tape stripping In another embodiment, sufficient electrical contact between the skin and the counter electrode is created by using a counter electrode having a large surface area. More specifically, a conductive polymeric path or metallic foil patch having an area much larger than the skin area exposed to the skin permeabilizing device is used The large area of the counter electrode in this embodiment decreases its impedances and allows accurate measurements of the electrical parameter of the area of skin exposed to the skin permeabilizing device In one specific embodiment a conductive band is wrapped around the subject's arm and used as the counter electrode In another embodiment, the counter electrode may be placed in a handle ofthe skin permeabilizing device, to which a subject grasps during operation In another embodiment, the counter electrode surrounds the skin permeabilizing device When the two electrodes are properly positioned, in step 806, an initial conductivity between the two electrodes is measured This may be accomplished by applying an electrical signal to the patch of skin through the electrodes In one embodiment, the electrical signal supplied may have sufficient intensity so that the electrical parameter of the skin can be measured, but have a suitably low intensity so that the electrical signal does not cause permanent damage to the skin, or any significant electrophoresis effect for the substance being delivered In one embodiment, a 10 Hz AC source is used to create a voltage differential between the source electrode and the counter electrode The voltage supplied should not exceed 500 mV, and preferably not exceed 100 mV, or there will be a risk of damaging the skin In another embodiment, an AC current source is used The current source may also be suitably limited The initial conductivity measurement is made after the source has been applied using appropriate circuitry In one embodiment a resistive sensor is used to measure the impedance of the patch of skin at 10 Hz. In another embodiment, a 1 kHz source is used Sources of other frequencies are also possible. In step 808, a skin permeabilizing device is applied to the skin at the first site Any suitable device that increases the permeability of the skin may be used In one embodiment, ultrasound is applied to the skin at the first site According to one embodiment, ultrasound having a frequency of 20 kHz and an intensity of about 10 W/cm 2 is used to enhance the permeability ofthe patch of skin to be used for transdermal transport In step 810, the conductivity between the two sites is measured The conductivity may be measured periodically, or it may be measured continuously The monitoring measurements are made using the same electrode set up that was used to make the initial conductivity measurement In step 812, mathematical analysis and/or signal processing may be performed on the time-variance of skin conductance data Experiments were performed on human volunteers according to the procedure above, with ultrasound used as the method of permeabilization Ultrasound was applied until the subjects reported pain Skin conductivity was measured once every second during ultrasound exposure After plotting the conductance data, the graph resembled a sigmoidal curve The conductance data was in a general sigmoidal curve equation C = Cι + ^ ] + - e *s< n ÷- where C is current, C, is current at t= 0, C/is the final current, S is a sensitivity constant, t is the exposure time required to achieve an inflection point, and t is the time of exposure Fig. 9 shows the time variation of the skin conductance while being exposed to ultrasound The curve is a sigmoidal curve and can be fitted to the above equation The line shown in Fig. 9 corresponds to a fit to the above equation The values of fitted parameters were obtained and are plotted The value of t* corresponds to an exposure time required to achieve an inflection point (a point where the slope of the curve shown changes sign) The inflection time approximately indicates the time required to achieve half the total exposure Fig. 10 shows a relationship between the inflection time and the pain time on various volunteers The data shows that the time to pain is proportional to the time to the inflection point on human volunteers In this figure, R2 is the correlation coefficient, where a R2=l indicates 100% correlation ofthe experimental data to the predicted values Based on this data, a method can be developed to predict the required ultrasound exposure time Referring to Figs. 11 and 12, a flowchart depicting a method of determining when to terminate the application of ultrasound, and corresponding example graphs, are provided In step 1 102, A/D conversion is performed on the conductivity data This results in a graph similar to the one in Fig. 12a Next, in step 1 104, filtering is performed on the digital data As shown in Fig. 12b, the filtered data has a smoother curve than the unfiltered data of Fig. 12a Next, in step 1 106, the slope of the curve is calculated In step 1 108, the maximum value for the slope is saved If the current value for the slope is greater than the maximum value that is saved, the maximum value is replaced with the current value Next, in step 1 1 10, if the slope is not less than or equal to the maximum value, the process returns to step 1 102 to wait for a peak If the slope is less than or equal to the maximum value, in step 1 1 12 the process detects a peak, or point of inflection, shown in Fig. 12c, then, in step 1 1 14, terminates the application of ultrasound to the skin In one embodiment, the detection of the peak may be validated This may be provided to ensure that the "peak" detected, in step 1 1 12, was not noise, but was actually a peak In other embodiments, ultrasound may be applied even after the inflection point is reached In one embodiment, ultrasound is applied for a predetermined time This predetermined time may be based on a percentage of the time to reach the inflection point For example, once the inflection point is reached, ultrasound continues to be applied for an additional 50% of the time it took to reach the inflection point Thus, if it took 14 seconds to reach the inflection point, ultrasound is applied for an additional 7 seconds Other percentages may be used, and this percentage may be based on factors including pain threshold and skin characteristics In another embodiment, ultrasound is applied until the slope decreases to a certain value Referring again to Fig. 11, after the inflection point is reached, the slope decreases as ultrasound is applied Thus, ultrasound may be applied until the slope decreases by a percentage, such as 50%, or to a predetermined value As above, this determination is flexible and may vary from individual to individual In another embodiment, the current at the inflection point is measured, and then a percentage of this current is still applied For example, if the inflection point is reached at 40 μamps, an additional 10% of this, for a total of 44 μamps, may be reached Again, this determination is flexible and may vary from person to person O 00/35357 - 35 - Referring again to Fig. 8, in step 814, the parameters describing the kinetics of skin conductance changes are calculated These parameters include, inter alia, skin impedance, the variation of skin impedance with time, final skin impedance, skin impedance at inflection time, final current, exposure time to achieve the inflection time, etc In step 816, the skin permeabilizing device applied in step 808 is terminated when desired values of the parameters describing skin conductance are achieved EXAMPLE In vitro experiments were performed in accordance with a method according to one embodiment of the present invention Pig skin was mounted on a diffusion cell and was exposed to ultrasound using 1% Sodium Lauryl Sulfate in water as a coupling medium Skin conductance was measured by placing two electrodes across the skin The impedances were measured at two frequencies at 10 Hz, which is near the ultrasound range, and 1 kHz The impedances measured at the frequencies differed by about 25 fold when the skin was impermeable Upon sonication, the difference between the impedances at the two frequencies decreased The differential impedance between the two frequencies decreased with time When the skin was highly permeable, the impedances at two frequencies differed by only about 20% SLS was removed from the chamber and the chamber was dried A gel was placed in the chamber in contact with the skin The gel was prepared by mixing glucose reagent from the Sigma™ kit 315 (10% by weight) into polyvinyl alcohol solution (20% by weight) in PBS The gel was kept in the freezer to allow cross linking The gel was clear in the beginning, and changed color to red when it came in contact with glucose 2 Extraction and Analysis of At Least One Analyte in Body Fluid According to another embodiment of the present invention, ultrasound may be used to extract body fluids through or out of skin that has its permeability increased Referring to Fig. 13, a flowchart depicting a method for extraction and analysis of at least one analyte in a body fluid according to one embodiment of the present invention is disclosed In step 1302, the permeability of the skin is increased This may be accomplished by any suitable method for increasing the permeability of the skin, such as iontophoresis In one embodiment, the permeability ofthe skin may be increased through the application of ultrasound As used herein, the term "interstitial fluid" may include lymph, interstitial fluid, and serum that may be extracted from the body. It is also used to describe components of interstitial fluid. In step 1304, interstitial fluid is extracted transdermally from the surface of the skin Extraction can be performed after sonication or other permeation methods using a wide variety of different forces These forces may include physical forces, chemical forces, biological forces, vacuum pressure, electrical, osmotic, diffusion, electro-magnetic, ultrasound, cavitation, mechanical, thermal, capillary forces, fluid circulation across the skin, electro-acoustic, magnetic, magneto-hydrodynamic, acoustic, convective dispersion, photo acoustic, by rinsing body fluid off skin, or by any combination of these forces. Spatial and/or temporal positive and/or negative pressure modulation may be used. In spatial modulation, positive pressure is applied to an area of the skin, while a vacuum is applied to another area, assisting in the extraction of body fluid. In temporal modulation, vacuum and positive pressure alternate at about the same area of skin, assisting in the extraction of body fluid. The application of either spatial or temporal modulation may be continuous or discontinuous, and they may be applied separately or in combination. In one embodiment, vacuum pressure may be applied to extract body fluid. Vacuum pressure may be applied continuously, or it may be applied discontinuously. When applied discontinuously, the vacuum may be applied in a pulsed fashion. A material that maintains the surface configuration of the skin (e.g., flat, convex, or concave), such as mesh, membrane, perforated metal, or other porous material, may be applied between the vacuum pressure and the skin while the vacuum pressure is applied. The vacuum can act through these structures and can be generated mechanically, electro-mechanically, chemically, or electro-chemically. In another embodiment, the vacuum can be applied in such a manner so as to maintain the skin surface configuration with the vacuum alone. In another embodiment, a chamber that is applied to skin can have a design (configuration and material properties) to localize high pressure gradient across skin and/or other tissues In another embodiment, electrical forces may be applied Electrical forces may be iontophoretic, electro-osmotic, or may be electroporation A gel with an electric charge also may be applied, in order to encourage the absorption and evacuation of body fluid and components thereof In another embodiment, osmotic forces may be used. A gel or solution may be applied to the skin surface in order to encourage osmosis In another embodiment, ultrasound may be used to pump body fluid and fluid components, to levitate, to activate gas bodies, to produce cyclic impulse mechanical stress to the skin, to create microstreaming, to increase temperature, or to set up standing waves Single or multiple sources of ultrasound may be used in combination with various characteristics of ultrasound, ejL . , different frequencies, intensities, or coupling media, in order to encourage the extraction of body fluid. In another embodiment, mechanical forces may used to extract body fluid These forces may be achieved by, inter alia, a roller, a squeezer, a stretcher, iris compressor/tensioner device, etc. to increase the volume ofthe body fluid that is extracted. In one embodiment, a tensioner is used to extract body fluid Referring to Fig. 14, which depicts an embodiment of a tensioner, tensioner 1402 consists of a convex geometry held against the skin 1404. By pressing tensioner 1402 against skin 1404, body fluid may be collected within cavity 1406 of tensioner 1402. In another embodiment, thermal forces may be used to extract body fluid. The skin temperature may be increased using electricity, chemical, ultrasonic, or optical energy sources or methods and/or utilize temperature sensitive polymers to swell or contract a gel, membrane, and/or solid to encourage the absorption and evacuation of body fluid and components thereof Temperature sensitive polymers may be used to move a piston or membrane to push or suck fluid Examples of such polymers include, inter alia, poloxymers In another embodiment, chemical forces are used Chemical substances may be used to augment convective and/or diffusive forces as a means to extract additional body fluid, and/or to enhance transpoπ and/or accumulation of body fluids at specific body sites A hydrogel with an incorporated, trapped, or immobilized bioactive molecules such as enzyme would allow for extraction by osmosis into a sensing scaffold In another embodiment, pH/ionic forces may be used These forces may be used to change the material properties and characteristics, e ^ _. hydrophilic material to a hydrophobic material A pH/ionic sensitive membrane and/or gel may be swollen and contracted in order to encourage the absorption and evacuation of body fluid and components thereof In another embodiment, capillary forces may be used These forces may be used to assist in fluid transport across skin pores Referring again to Fig. 13, in step 1306, the body fluid and components thereof are collected This collection may be accomplished by absoφtion, adsorption, phase separation, mechanical forces, electrical forces, chemically induced forces, or a combination thereof. Preferably, a humid environment is created and maintained in order to control evaporation of analytes during extraction The collected volume of body fluid may be the same as the volume extracted, or it may be a fixed constant volume In one embodiment, absorption or adsorption may be used In this embodiment, the body fluid may be collected into a gel, which contains a captive enzyme. A polymeric, metallic, or ceramic screen, scaffold, mesh, or membrane, or a combination may be used to do this These materials may also be a component of a sensor. In one embodiment, phase separation may be used. Body fluid may be isolated by combining the fluid with an appropriate density immiscible fluid The body fluid may be collected into a conical chamber. Another use of phase separation is achieved by first applying a hydrophobic coating on the skin prior to the extraction step. After the extraction, body fluid is present in the form of droplets on the hydrophobic coating. In another embodiment, mechanical forces may be used to collect body fluids This includes forces such as vacuum, pressure, and acoustic forces Dispersed body fluid may be collected over a greater area to a smaller area using a microfluidic channel against the skin A means to evacuate the fluidic path may include the introduction of a liquid and/or gas This means to evacuate may be applied to all collection processes, and not just mechanical collection In another embodiment, electrical collection may be used In this embodiment, solid, liquid droplets, or gas are charged and transported (moved) from skin to a sensor or to a collecting compartment using electrical forces In another embodiment, chemical collection may be used A hydrophilic gel may be used to collect body fluids The material properties and characteristics may be changed, e_g_, hydrophilic material to a hydrophobic material, in order to encourage the absorption and evacuation of body fluid and components thereof In another embodiment, capillary collection may be used Body fluid may be collected into a capillary or capillaries This allows for quantitative volume or a method to move fluid to a sensor The capillary or capillaries may be filled with multiple fibers to increase the surface area on which a liquid's adhesive forces can act This method may be used in conjunction with a chemical substance and/or other driving forces In step 1308, the concentration of analytes in body fluid is sensed Sensing the concentration of an analyte present in body fluid may be accomplished by employing electrochemical, optical, acoustical, biological, and enzymatic technology in combination or alone A sensor or sensors can be disposable, replenishable, discrete, or continuous According to one embodiment, a sensing device may have a sensor or sensors capable of detecting more than one analyte If one or more, or a combination of several analytes exists in stable and/or predictable physiologic concentrations, the ratio of one analyte to the other would allow for concentration detection and self-calibration Neither the volume of the body fluid or the dilute volume needs be known A sensing device presented with a known volume of body fluid (undiluted) and a known volume of diluent would not require frequent calibration In one embodiment, body fluid is extracted and optical analysis is performed on the body fluid In another embodiment, the body fluid is extracted and electrochemical analysis is performed on the body fluid In another embodiment, the body fluid is extracted and the acoustical emission of an analyte undergoing a chemical reaction is detected and analyzed In another embodiment, the body fluid is extracted, and living cells may be used to sense a concentration of an analyte in body fluid In another embodiment, the body fluid is extracted, and thermal analysis is performed on the body fluid In step 1310, information is provided for the user interface A user interface may provide features for both daytime and nighttime monitoring In one embodiment, this may include alarms for high/low analyte concentrations, may provide access to trends and history, and may enable a prediction of future concentration values The user interface may provide the ability to download history Other convenience features, such as a low battery indicator, may be included in the user interface The battery may be solar, nickel cadmium, standard alkaline, or lithium ion In another embodiment, after the permeability of the skin is increased, the presence of the analyte may be sensed without extraction Infrared light, for example, may be used to sense the presence of an analyte, with less interference from H 2 0 In another embodiment, after the permeability of the skin is increased, at least one analyte is permitted to passively diffuse through the skin The analyte may be collected in a gel used as a collecting device, and a sensing device, attached to the gel, may be used to sense the presence ofthe analyte In another embodiment, additional methods for generating cavitation and convectional flow may be applied with, before, after, or instead of the ultrasound application for skin permeabilization and/or extraction and/or collection steps These methods include the use of a propeller, fly wheel, transverse needle, and local shear induced permeabilization In another embodiment, the non-invasive method disclosed herein may be used to determine the level of blood glucose Referring to Fig. 15, in step 1502, the permeability of the skin is increased This may be achieved by any suitable method Preferably, ultrasound is applied as discussed above In step 1504, the interstitial fluid is extracted from the skin This may be accomplished by any suitable method, including those discussed above Preferably, a vacuum is applied to extract the interstitial fluid from the skin In step 1506, the interstitial fluid is collected This may be accomplished by any suitable method, including those discussed above Preferably, the interstitial fluid is collected into a gel containing glucose sensitive reagents The gel may change color when it comes in contact with glucose In step 1508, the color change of the gel is monitored to determine the glucose concentration in the interstitial fluid. In another embodiment, ultrasound may be used for detection (evaluation, follow up treatments) of skin and/or other subcutaneous abnormalities presented by pathological concentrations of specific analytes, or detection of specific components administered to the site and detection of their elimination or conversion (psoriasis, skin malignancies, etc ) This approach may be used, for example, in extracting analytes or reagents from skin-affected sites (lesion plaques), tumors, etc In another embodiment, the delivery and/or removal of endogenous and non-endogenous components from the skin by the application by a force is disclosed Forces, such as ultrasound, electrical, magnetic, capillary, mechanical, chemical, electromagnetic, osmotic, concentration gradient, or combinations thereof may be used in applications such as, inter alia, removal of residual surfactant, cavitation enhancers, tattoo bleach, and botox (remove from forehead, and neck lines, reduce sweating) In another embodiment, sensing components may be delivered into the skin to analyze interstitial fluid components in situ The sensing components may also be delivered into the skin to measure emitted products or reagents of any sensing reaction (chemical or enzymatic) 3 Sonophoretic Drug Delivery A drug is defined as a therapeutic, prophylactic, or diagnostic molecule or agent, that may be in a form dissolved or suspended in a liquid, solid, or encapsulated and/or distributed in or within micro or nanoparticles, emulsion, liposomes, or lipid vesicles Drug delivery is defined as the delivery of a drug into blood, lymph, interstitial fluid, cells, tissues, and/or organs, or any combination thereof Referring to Fig. 16, an active patch drug delivery apparatus 1602 that is attached to skin 1600 is depicted Drug delivery apparatus 1602 includes patch 1604 Patch 1604 includes adhesive 1610, drug molecules 1612 and transducer 1614 Patch 1604 is an active patch Adhesive 1610 acts as an attaching device Alternatively, the attaching device may be a vacuum, band, or strap As transducer 1614 oscillates, the permeability of skin 1600 is increased in accordance with the present invention and drug molecules 1612 are delivered to and/or through skin 1600, or/and after skin 1600 is permeabilized, drug molecules 1612 are transported through skin 1600 to the capillaries and blood vessels below skin 1600 A limiting step membrane 1613 may be located between skin 1600 and drug molecules 1612 Transducer 1614 preferably operates at a frequency in the range of between 20 kHz to 2 5 MHz, using appropriate electrical signal generators and amplifiers Transducer 1614, more preferably, is operating at a frequency in the range of between 20 and 200 kHz Other ultrasound parameters include, but are not limited to, amplitude, duty cycle, distance from the skin, coupling agent composition, and application time and may be varied to achieve sufficient enhancement of transdermal transport The intensity preferably varies from 0 to 20 W/cm 2 Further, transducer 1614 may be configured as a cylinder, a hollow cylinder, a hemispherical configuration, conical configuration, planer configuration or rectangle configuration Transducer 1614 may also consist of an array of acoustic elements that are swept in time Transducer 1614 may be comprised of quartz, PVDF, ceramic including PZT and screen printed ceramic, magnetostrictive, or composite material including molded ceramic and benders Transducer 1614 may be used alone, or in conjunction with other forces, or contributors, to enhance drug delivery These other forces, or contributors, include, but are not limited to, a magnetic field including electromagnetic forces, an electrical current or iontophoresis, mechanical skin manipulation, chemical enhancement, heat, and osmotic forces Transducer 1614 administers ultrasound preferably at frequencies of less than or equal to about 2 5 MHz, preferably at a frequency that is less than 1 MHz, and more typically in the range of about 20 to 100 kHz Exposures to ultrasounds from transducer 1614 are typically between about 5 seconds and about 10 minutes continuously, but may be shorter and/or pulsed, for example, at 100 to 500 msec pulses every seconds for a time sufficient to permeabilize the skin The ultrasound intensity is of a level that preferably does not raise skin 1600's temperature more than about 1 to 2 degrees Centigrade and does not cause permanent damage to the skin The intensity typically is less than 20 W/cm , preferably less than 10 W/cm 2 Intensity in time of application is inversely proportional to exposure time, so that high intensities are applied for shorter period of times in order to avoid skin damage It should be noted that although normal low range ultrasound is 20 kHz, comparable results are achieved by varying the frequency to less than 20 kHz, or into the sound region The time needed for permeabilization is dependant upon the frequency and intensity of the ultrasound from transducer 1614 and the condition of skin 1600. At 20 kHz, for example, an intensity of 10 W/cm 2 , with a duty cycle of 50 percent, skin 1600 is permeabilized sufficiently in about 5 minutes if skin 1600 is on a human forearm Permeabilizing ultrasound may be applied for a predetermined amount of time or may be applied only until permeabilization is attained Because skin 1600 characteristics or properties may change over time, based on aging, diet, stress, and other factors, it may be preferable to measure permeability as ultrasound is applied to minimize the risk of skin 1600 damage Several methods may be used to determine when sufficient permeabilization has been reached One method measures relative skin conductivity at the permeabilization site versus a reference point These measurements are performed by applying a small AC or DC electric potential across two electrically isolated electrodes in contact with skin 1600 Electric current flowing through these electrodes is measured using an ammeter and skin 1600 resistance is measured using the values ofthe potential and current Drug delivery patch apparatus 1602 may serve as one of the electrically isolated electrodes in contact with skin 1600 Preferably, drug delivery patch apparatus 1602 permeabilizes skin 1600 prior to the conductivity tests Another way to determine when sufficient permeabilization has been reached is to measure conductivity Fully permeabilized skin has a resistance of no more than about 5 kΩ measured across approximately 1 7 cm 2 Another method is to detect and/or quantitate the transdermal movement of an analyte, such as creatinine, calcium or total ions, that is present in interstitial fluid in a fairly constant amount, and may be used either to calibrate the concentration of analyte to be extracted and quantified, or as a measure of permeabilization The higher the constant analyte flux, the greater degree of permeabilization The degree of permeability also may be monitored using a sensor attached to drug delivery patch apparatus 1602 that determines the concentration of drug molecules 1612 being delivered or an analyte being extracted As the permeability increases, the drug concentration within drug delivery patch 1602 decreases Drug delivery patch apparatus 1602 also may be applied to pretreated skin 1600 In other words, permeabilization of skin 1600 is already achieved Drug delivery patch apparatus 1602 is placed over pretreated skin 1600 to deliver drug molecules 1612 Any known device may be used to pre-treat skin 1600, including, but not limited to, physical forces, chemical forces, biological forces, vacuum pressure, electrical forces, osmotic forces, diffusion forces, electromagnetic forces, ultrasound forces, cavitation forces, mechanical forces, thermal forces, capillary forces, fluid circulation across the skin, electro-acoustic forces, magnetic forces, magneto-hydrodynamic forces, acoustic forces, convective dispersion, photo- acoustic forces, by rinsing body fluid off skin, and any combination thereof Drug molecules 1612 include a variety of bio-active agents, including protein and peptides Other materials include nucleic acid molecules such as vaccines including therapeutic proteins, synthetic organic and inorganic molecules including anti-inflammatories, anti-virals, anti-fungal, anti-biotics, and local anesthetics, and saccharides and polysaccharides Drug molecules 1612 may be administered in an appropriated pharmaceutically acceptable carrier having an absorption coefficient, similar to water, such as an aqueous gels, ointment, lotion, or suspension Drug molecules 1612 also may be contained with adhesive 1610 that attaches to skin 1600 Further, drug molecules 1612 also may be encapsulated or suspended in a liquid, gel, or solid matrix within patch 1604 Drug delivery patch apparatus 1602 also includes a battery 1616 Battery 1616 acts as a power source for transducer 1614 Battery 1616 provides a relatively high energy burst Drug delivery patch apparatus 1602 also includes electronic coupling 1618 that serves as the drive electronics for drug delivery patch apparatus 1602 Drug delivery patch apparatus 1602 also includes user interface 1620 In one embodiment, patch 1604 includes transducer 1614, drug molecules 1610, and adhesive 1610 In another embodiment, patch 1604 includes transducer 1614, drug molecules 1612, adhesive 1610, battery 1616, electronic coupling 1618, and user interface 1620 In another embodiment, patch 1604 includes transducer 1614, drug molecules 1612, adhesive 1610, and battery 1616. In another embodiment, adhesive 1610 is to the side of transducer 1614 and drug molecules 1612. Battery 1616, electronic coupling 1618, and user interface 1620, may be located elsewhere on a user and in communication with patch 1604 via hard wire or telemetry In another embodiment, user interface 1620 may be located elsewhere on the user and is in communication with patch 1604 via hard wire, telemetry, infrared, or fiber optic means Thus, the elements of drug delivery apparatus 1602 may be detachable and portable from each other Further, any ofthe components of drug delivery apparatus 1602 may be disposable or reusable For example, patch 1604, which includes transducer 1614, drug molecules 1612 and adhesive 1610 may be disposed after detachment from skin 1600 However, battery 1616, electronic coupling 1618, and user interface 1620 may be re-usable with further patches 1604 In one embodiment, transducer 1614 operates alone to push drug molecules 1612 through and to skin 1600 Alternatively, drug delivery patch apparatus 1602 and transducer 1614 may operate in conjunction with a driving force that further facilitates the transdermal transport of drug molecules 1612 These forces include, but are not limited to physical forces, chemical forces, biological forces, vacuum pressure, electrical forces, osmotic forces, diffusion forces, electromagnetic forces, ultrasound forces, cavitation forces, mechanical forces, thermal forces, capillary forces, fluid circulation across the skin, electro-acoustic forces, magnetic forces, magneto-hydrodynamic forces, acoustic forces, convective dispersion, photo-acoustic forces, by rinsing body fluid off skin, and any combination thereof Referring to Fig. 17, an embodiment of transducer 1614 is depicted Transducer 1614 may be an array of acoustic elements that are swept in time as ultrasound is applied to drug molecules 1612, and through adhesive 1610 to skin 1600 Acoustic elements 1700 comprise transducer 1614 Elements 1700 are depicted as squares within a larger square Elements 1700 are not limited to this configuration and may be configured as a cylinder, a hollow cylinder, hemispherical, conical, planer, rectangular Each acoustic element of elements of 1700 may be swept individually or within a group as transducer 1614 is activated For example, element A activates, followed by elements B and E, then followed by elements C, F, and I, and so on Element P may be activated last as transducer 1614 is swept Further, acoustic elements 1700 may comprise fingers Referring to Fig. 17, a finger may be depicted as elements A, E, I, and M Each finger may be activated or swept in time Acoustic elements 1700 may be configured to channel the ultrasound energy from transducer 1614 to a specified area in 100 smaller than the area of transducer 1614 Referring to Fig. 18, patch 1604 and user interface 1620 are coupled to feedback mechanism 1802 Feedback mechanism 1802 may be detachable from user interface 1620 Alternatively, feedback mechanism 1802 may be contained within user interface 1620 Thus, feedback mechanism 1802 may be contained within drug delivery patch apparatus 1602 Feedback mechanism 1802 provides for programming of drug delivery rates or pre-set doses of drug molecules 1612 Feedback mechanism 1802 also may provide memory to record or display historical delivery data to user interface 1620 Feedback mechanism 1802 communicates the on time of transducer 1614 to user interface 1620 for display to the user Feedback mechanism 1802 also may provide alarms for low drug molecules 1612 and/or low power in battery 1616 Thus, feedback mechanism 1802 alerts a user via a user interface 1620 that drug molecules 1612 and patch 1604 needs to be replenished or that drug delivery patch apparatus 1602 is low on power Feedback mechanism 1802 also may monitor the amount of drug molecules 1612 delivered via transdermal transport Feedback mechanism 1802 also may monitor the amount of ultrasonic energy, or other driving forces listed above, applied to skin 1600 by transducer 1614 Limits may be set in feedback mechanism 1802 to limit the ultrasound energy from transducer 1614 so as to no irritate or damage skin 1600 Feedback mechanism 1802 also may monitor the concentration of drug molecules 1612 remaining in patch 1604 Feedback mechanism 1802 also may monitor the concentration of drug molecules or analytes in the interstitial fluid, blood, and other body fluids Feedback mechanism 1802 also may monitor the amount of cavitation produced by the application of ultrasound energy Feedback mechanism 1802 also may monitor the degree of physiological effects such as blood pressure, EMG, EEG, and ECT feedback in order to measure delivery of drug molecules 1612 Feedback mechanism 1820 also may provide connections with additional patches or testing devices in order to perform conductivity tests 4 Transdermal Vaccination by Sonophoresis Generally, vaccines are administered for the prevention, amelioration or treatment of infectious diseases Vaccines are commonly used to provide immunity from diseases such as influenza, poliomyelitis, varicella zoster (chicken pox), measles, as well as several other diseases A vaccine is generally made from an antigen isolated or produced from the disease-causing microorganism An antigen is defined as "anything that can be bound by an antibody " This can be an enormous range of substances from simple chemicals, sugars, small peptides to complex protein complexes, such as viruses The small antigens are not, however, immunogenic in themselves, and need to be coupled to a carrier to elicit an immune response Typically, the vaccine is delivered to the bloodstream by an invasive method, such as an injection The B cells in the blood stream respond to the antigen by producing antibodies These antibodies bind to the antigen to "neutralize," or inactivate it Memory cells are also produced, and remain ready to mount a quick protective immune response against subsequent infection by the same disease- causing agent. Immunization is the process of causing immunity by injecting antibodies or provoking the body to make its own antibodies against a certain microorganism Immunization may be a result of a vaccination As discussed above, the use of ultrasound to facilitate transdermal transport is known The mechanism by which ultrasound is used to facilitate transdermal transport has differed In the context of transdermal delivery systems, ultrasound was initially a driving force that essentially pushed drugs through the skin and into the circulatory system Ultrasound also increases the permeability of the skin In other words, application of ultrasound having a particular frequency disorganizes the lipid bi-layer in the skin, thereby increasing the permeability of the skin In this context, drugs may be delivered to the body through the skin, or an analyte may be extracted from the body through the skin System and methods for the application of ultrasound to enhance the permeability of skin, as well as the extraction of body fluids are discussed, above Although the permeability of the skin is increased by the application of ultrasound, a driving force is still required for transdermal transport, but the required intensity of the driving force is decreased For example, a concentration gradient is generally a sufficient driving force for transdermal transport through skin whose permeability has been enhanced using ultrasound The permeability enhancement that results from the application of ultrasound is due at least in part, to cavitation that is caused by the ultrasound Fig. 19 depicts a method for transdermal vaccination by sonophoresis according to one embodiment of the present invention. Referring to Fig. 19, in step 1902, the permeability of the skin is increased. This may be achieved by several methods, including those discussed above. In one embodiment, ultrasound may be applied at about 10 W/cm 2 , with a duty cycle of about 50%. Ultrasound may be applied at a distance from the skin of about 0.5 mm to 1 cm, and for an application time of from about 30 seconds to about 5 minutes. A coupling medium may be used between the transducer and the skin, and may contain aqueous or non-aqueous chemicals including, but not limited to, water, saline, alcohol, including ethanol and isopropanol (1 -100% mixtures with saline), surfactants, fatty acids such as linoleic acid (0.1-2% mixtures in ethanol- water (50:50) mixture), azone (0.1-10% mixtures in ethanol-water (50:50) mixture), 01.-50% polyethylene glycol in saline, 1 -100 mM EDTA, EGTA, or 1% SLS and silica particles. The coupling media provide effective transfer of ultrasound energy from transducer to the skin. Appropriate mixtures of these coupling media may also enhance cavitation activity inside, on the surface, or near the skin, thus inducing more effective transport of molecules across the skin. In step 1904, after the permeability of the skin is increased, sonication is terminated, and a vaccine is provided on the permeated skin. In one embodiment, the vaccine may be incorporated into a transdermal patch. Other forms ofthe vaccine, such as gels and liquids, may also be used. The vaccine may comprise as the active ingredient a peptide, protein, allergen, or other antigen, or DNA encoding any of the foregoing and may also include other adjuvants normally employed. These vaccines may be used as cancer vaccines, tetanus vaccines, etc. In step 1906, the vaccine is delivered to the skin cells. In one embodiment, the vaccine is delivered to skin cells, including Langerhans cells, dendritic cells, and keratinocytes. In one embodiment, the vaccine is delivered to the Langerhans cells. The Langerhans cells are the cells responsible for capturing a vaccine and presenting it to the Lymphatic system, and eliciting an immune response The vaccine may be delivered to other cells to illicit an immune response In one embodiment, the vaccine may diffuse to the skin cells, including Langerhans cells, dendric cells, and keratinocytes Once the vaccine is received by the skin cells, the vaccine is transported to the lymph nodes efficiently, increasing the efficiency of vaccination In another embodiment, the vaccine is transported transdermally through, in, or into the skin and into the bloodstream, wherein it acts as if it were injected in a conventional manner In another embodiment of the present invention, the vaccine is provided simultaneously with the application of ultrasound The ultrasound in this embodiment is used both to permeabilize the skin, as well as and to deliver the vaccine transdermally to the Langerhans cells The ultrasound acts as a driving force Examples of using ultrasound to transport drugs from a patch are discussed above In another embodiment of the present invention, ultrasound is applied to the skin to increase the permeability of the skin. Once the vaccine is provided, additional driving forces are provided to deliver the vaccine to the body Examples of driving forces include, inter alia, physical forces, chemical forces, biological forces, vacuum, electrical forces, osmotic forces, diffusion forces, electro-magnetic forces, ultrasound forces, cavitation forces, mechanical forces, thermal forces, capillary forces, fluid circulation across the skin, electro-acoustic forces, magnetic forces, magneto-hydrodynamic forces, acoustic forces, convective dispersion, photo acoustic forces, and any combination thereof In another embodiment, ultrasound can be used to induce irritation and inflammation of the skin Inducing irritation and inflammation may make the vaccine placed on the skin more effective in inducing an immune response. In another embodiment, chemical enhancers may be used to increase the permeability ofthe skin Although the present invention has been described in detail, it should be understood that various changes, substitutions, and alterations can be made without departing from the intended scope as defined by the appended claims.

Claims: CLAIMS What is claimed is 1 A method for enhancing transdermal transport, comprising increasing a permeability of an area of a membrane with a permeabilizing device, monitoring the permeability ofthe area of membrane, transporting a substance into and through the area of membrane 2 The method of claim 1 , wherein the step of increasing a permeability of an area of membrane with a permeabilizing device comprises applying electricity to the area of membrane, measuring at least one electrical parameter ofthe area of membrane, and controlling the permeabilizing device based on the at least one electrical parameter 3 The method of claim 1 , wherein the step of increasing a permeability of an area of membrane with permeabilizing device comprises creating a volume of fluid adjacent the area of membrane, said fluid having an initial concentration of a first substance, and applying the permeabilizing device to the area of membrane 4 The method of claim 3, wherein the step of monitoring the permeability ofthe area of membrane comprises monitoring changes in the concentration ofthe first substance, and controlling the permeabilizing device based on the changes in the concentration ofthe substance 5 The method of claim 1 , wherein the step of increasing a permeability of an area of membrane with a permeabilizing device comprises creating a volume of fluid adjacent the area of membrane, determining a reference value for an electrical parameter of the volume of fluid, and applying the permeabilizing device to the area of membrane 6 The method of claim 5, wherein the created volume fluid is selected from the group consisting of a liquid, a gel, and a solid 7 The method of claim 5, wherein the step of monitoring the permeability ofthe area of membrane comprises monitoring changes in the electrical parameter ofthe volume of fluid, and controlling the permeabilizing device based on the changes in the electrical parameter of the volume of fluid 8 The method of claim 1 , wherein the step of increasing a permeability of an area of membrane with a permeabilizing device comprises providing a first electrode in electrical contact with a first area of membrane, providing a second electrode in electrical contact with a second area of membrane, measuring an initial conductivity between said electrodes, and applying the permeabilizing device to said first area of membrane 9 The method of claim 8, wherein the step of monitoring the permeability ofthe area of membrane comprises measuring a second conductivity between said first and second electrodes, processing said initial conductivity and said second conductivity to establish information on a time-variation of membrane conductance, calculating at least one parameter describing a kinetics of membrane conductance changes responsive to said information, and terminating said application of said permeabilizing device in response to a desired value for said at least one parameter 10 The method of claim 1 , wherein the step of transporting a substance across an outer surface ofthe area of membrane comprises extracting a body fluid from or through said area of membrane, collecting said body fluid, and sensing the presence of said at least one analyte in said body fluid 1 1 The method of claim 1 , wherein the substance is a drug 12 The method of claim 1 , wherein the substance is a vaccine 13 The method of claim 1, wherein the substance includes at least one component of interstitial fluid 14 The method of claim 1 , wherein the permeabilizing device is an ultrasound-producing device 15 The method of claim 1 , wherein the permeabilizing device is a device that produces a force selected from the group consisting of chemical, electroporation, mechanical, disrupting, tape stripping, and laser forces 16 The method of claim 1 , wherein the membrane is selected from the group consisting of biologic skin and synthetic skin 17 A method for enhancing a permeability of an area of skin comprising increasing the permeability of the area of skin with a skin permeabilizing device, applying electricity to the area of skin, measuring at least one electrical parameter ofthe area of skin, and controlling the skin permeabilizing device based on the at least one electrical parameter 18 The method of claim 17, further comprising applying electricity to the area of skin before increasing the permeability of the area of skin with a skin permeabilizing device, and measuring a baseline for the at least one electrical parameter 19 The method of claim 17, wherein the step of applying electricity to the area of skin comprises applying a first source having a first frequency to the area of skin, and applying a second source having a second frequency to the area of skin 20 The method of claim 19, wherein the step of measuring a first electrical parameter comprises measuring the at least one electrical parameter at the first frequency, and measuring the at least one electrical parameter at the second frequency 21 The method of claim 17, further comprising coupling a first electrode with the area of skin, coupling a second electrode with the skin, and measuring the at least one electrical parameter using the first electrode and the second electrode 22 The method of claim 21 , wherein the first electrode is coupled with a portion of stratum corneum ofthe area of skin 23 The method of claim 22, wherein the second electrode is coupled with a portion of epidermis over which the stratum corneum has been removed 24 The method of claim 22, wherein the second electrode is coupled with a portion of stratum corneum ofthe skin 25 The method of claim 21 , wherein at least one of the first and second electrode is coupled through a conductive medium 26 The method of claim 25, wherein the conductive medium a gel 27 The method of claim 25, wherein the conductive medium is a liquid 28 The method of claim 21 , wherein the second electrode is placed on the skin 29 The method of claim 17, further comprising comparing the at least one electrical parameter with the baseline, and wherein the step of controlling the skin permeabilizing device comprises discontinuing the application of the skin permeabilizing device based on the comparison 30 The method of claim 19, wherein the first source comprises a voltage source having a frequency of about 10 Hz 31 The method of claim 19, wherein the second source comprises a voltage source having a frequency of about 1 kHz 32 The method of claim 17, wherein the parameter is selected from the group consisting of impedance, conductance, capacitance, current, and voltage 33 The method of claim 17, wherein said skin permeabilizing device comprises an ultrasound-producing device 34 The method of claim 20, wherein the step of controlling the skin permeabilizing device comprises decreasing a characteristic of the skin permeabilizing device when the measurement ofthe at least one electrical parameter at the first frequency and, the measurement of the at least one electrical parameter at the second frequency differ by less than a predetermined amount, the characteristic selected from the group consisting of intensity and duty cycle 35. The method of claim 19, wherein the step of controlling the skin permeabilizing device comprises decreasing a characteristic of the skin permeabilizing device when a rate of change between the one electrical parameters reaches a predetermined value, the characteristic selected from the group consisting of intensity and duty cycle. 36. An apparatus for enhancing permeability of an area of skin comprising: a skin permeabilizing device configured to increase a permeability of the area of skin; an electrical source operable to apply electricity to the area of skin; a circuit to measure at least one electrical parameter ofthe area of skin; and a controller responsive to the circuit and operable to control the skin permeabilizing device. 37. The apparatus of claim 36, wherein the electrical source comprises: a first source having a first frequency; and a second source having a second frequency. 38. The apparatus of claim 37, wherein the circuit measures the at least one electrical parameter of the area of skin at the first frequency and to measure the at least one electrical parameter ofthe area of skin at the second frequency. 39. The apparatus of claim 36, further comprising: a first electrode coupled on the area of skin; and a second electrode positioned on the skin; wherein the circuit measures the first parameter of the area of skin between the first electrode and the second electrode. 40. The apparatus of claim 39, wherein the first electrode comprises an electrode that is coupled to a portion of stratum corneum ofthe area of skin. 41. The apparatus of claim 40, wherein the second electrode comprises an electrode that is positioned on a portion of epidermis over which the stratum corneum has been removed. 42 The apparatus of claim 39, wherein the first electrode comprises an electrode having a first surface area and coupled to a portion of stratum corneum of the area of skin 43 The apparatus of claim 42, wherein the second electrode comprises an electrode having a second surface area positioned on a portion of stratum corneum of the skin, and further wherein the second surface area is substantially larger than the first surface area 44 The apparatus of claim 40, wherein the controller compares the measurement of the at least one electrical parameter at the first frequency with the measurement of the at least one electrical parameter at the second frequency and turn off the skin permeabilizing device when they differ by less than about a predetermined amount 45 The apparatus of claim 37, wherein the first source comprises a voltage source having a frequency of about 10 Hz 46 The apparatus of claim 37, wherein the second source comprises a voltage source having a frequency of about 1 kHz 47 The apparatus of claim 38, wherein the parameter is selected from the group consisting of impedance, conductance, capacitance, current, and voltage 48 The apparatus of claim 37, wherein the controller compares the measurement of the at least one electrical parameter at the first frequency with the measurement of the at least one electrical parameter at the second frequency and decrease an intensity of the skin permeabilizing device source when they differ by less than about a predetermined amount 49 The apparatus of claim 38, wherein the controller compares the measurement of the at least one electrical parameter at the first frequency with the measurement of the at least one electrical parameter at the second frequency and decrease a duty cycle of the skin permeabilizing device source when they differ by less than about a predetermined amount 50 A method for enhancing permeability of an area of skin comprising creating a volume of fluid adjacent the area of skin, said fluid having an initial concentration of a first substance, applying a skin permeabilizing device to the area of skin, monitoring changes in the concentration ofthe first substance, and controlling the skin permeabilizing device based on the changes in the concentration ofthe substance 51 The method of claim 50, wherein the volume of fluid is selected from the group consisting of a liquid, a gel, and a solid 52 The method of claim 50, wherein the first substance comprises an analyte 53 The method of claim 52, wherein the step of controlling the skin permeabilizing device comprises discontinuing the application of the skin permeabilizing device when the concentration of analyte in the volume of fluid increases to a predetermined concentration 54 The method of claim 50, wherein the step of controlling the skin permeabilizing device comprises discontinuing the application of the skin permeabilizing device when a rate of increase of analyte in the volume of fluid reaches a predetermined concentration 55 The method of claim 50, wherein the step of controlling the skin permeabilizing device comprises decreasing an characteristic of skin permeabilizing device when the concentration of analyte in the volume of fluid increases to a predetermined concentration, said characteristic selected from the group consisting of intensity and duty cycle 56 The method of claim 50, wherein the first substance comprises a benign substance and the initial concentration comprises a concentration higher than that found in the body. 57 The method of claim 56, wherein the step of controlling the skin permeabilizing device comprises discontinuing the application of the skin permeabilizing device when the concentration of the benign molecule in the volume of fluid decreases to a predetermined concentration 58 The method of claim 56, wherein the step of controlling the skin permeabilizing device comprises discontinuing the application of the skin permeabilizing device when the rate of change in the concentration of the benign molecule in the volume of fluid reaches a predetermined value. 59. The method of claim 56, wherein the step of controlling the skin permeabilizing device comprises decreasing an intensity of the skin permeabilizing device when the concentration of the benign molecule in the volume of fluid decreases to a predetermined concentration. 60. The method of claim 56, wherein the step of controlling the skin permeabilizing device comprises decreasing an intensity of the skin permeabilizing device when the rate of change in the concentration of the benign molecule in the volume of fluid reaches a predetermined value. 61. The method of claim 56, wherein the step of controlling the skin permeabilizing device comprises decreasing the intensity of a duty cycle of the skin permeabilizing device when the concentration of the benign molecule in the volume of fluid decreases to a predetermined concentration. 62. A method for enhancing permeability of an area of skin comprising: creating a volume of fluid adjacent the area of skin; determining a reference value for an electrical parameter of the volume of fluid; applying a skin permeabilizing device to the area of skin; monitoring changes in the electrical parameter ofthe volume of fluid; and controlling the skin permeabilizing device based on the changes in the electrical parameter ofthe volume of fluid. 63. The method of claim 62, wherein the volume of fluid is selected from the group consisting of a liquid, a gel, and a solid. 64. The method of claim 62, wherein the electrical parameter is conductivity. 65. A method for regulating the permeabilization of an area of skin, comprising: providing a first electrode in electrical contact with a first area of skin; providing a second electrode in electrical contact with a second area of skin, measuring an initial conductivity between said electrodes, applying a skin permeabilizing device to said first area of skin, measuring a second conductivity between said first and second electrodes, processing said initial conductivity and said second conductivity to establish information on a time- variation of skin conductance, calculating at least one parameter describing a kinetics of skin conductance changes responsive to said information, and terminating said application of said skin permeabilizing device in response to a desired value for said at least one parameter 66 The method of claim 65, wherein said application of said skin permeabilizing device is continuous 67 The method of claim 65, wherein said application of said skin permeabilizing device is discontinuous 68 The method of claim 65, wherein said skin permeabilizing device comprises an ultrasound-producing device 69 The method of claim 65, wherein said step of calculating at least one parameter describing a kinetics of skin conductance changes responsive to said information comprises determining a slope of said information on a time-variation of skin conductance, and determining a point of inflection for said information on a time- variance of skin conductance 70 The method of claim 67, further comprising the steps of performing analog to digital conversion on said information on a time-variation of skin conductance, and filtering said digital data 71 The method of claim 65, wherein said step of processing said initial conductivity and said second conductivity to establish information on a time- variation of skin conductance comprises fitting said information into the following equation where (C f - C, ) = a + i + e s " C is current, C, is current at t= 0, C is the final current, S is a sensitivity constant, t is the exposure time required to achieve an inflection point; and t is the time of exposure 72 A method for extraction and analysis of at least one analyte in a body fluid, comprising increasing a permeability level of an area of skin, extracting a body fluid from or through said area of skin, collecting said body fluid, and sensing the presence of said at least one analyte in the body fluid. 73 The method of claim 72, wherein said step of increasing a permeability level of an area of skin comprises applying ultrasound to said portion of skin 74 The method of claim 72, wherein said step of extracting a body fluid from or through said area of skin comprises applying a force selected from the group consisting of physical forces, chemical forces, biological forces, vacuum, electrical forces, osmotic forces, diffusion forces, electro-magnetic forces, ultrasound forces, cavitation forces, mechanical forces, thermal forces, capillary forces, fluid circulation across the skin, electro-acoustic forces, magnetic forces, magneto- hydrodynamic forces, acoustic forces, convective dispersion, photo acoustic forces, by rinsing body fluid off skin, and any combination thereof 75 The method of claim 74, wherein said vacuum force is applied continuously 76 The method of claim 74, wherein said vacuum force is applied discontinuously 77 The method of claim 74, wherein a material is placed between said vacuum force and said skin in order to maintain a surface configuration of said skin 78 The method of claim 77, wherein said material is selected from the group consisting of mesh, membrane, and perforated metal 79 The method of claim 77, wherein said vacuum force is generated by a device selected from the group consisting of mechanical, electro-mechanical, chemical, or electro-chemical 80 The method of claim 74, wherein said electrical force is selected from the group consisting of iontophoretic, electro-osmotic, and electroporation 81 The method of claim 74, wherein a gel is applied to said skin in order to encourage osmosis 82 The method of claim 74, wherein said ultrasound force is applied to create a result, said result selected from the group consisting of to pumping body fluid and fluid components, levitating, activating gas bodies, producing cyclic impulse mechanical stress to the skin, creating microstreaming, increasing temperature, and setting up standing waves 83 The method of claim 74, wherein a plurality of ultrasound-producing devices are used to create said ultrasound force 84 The method of claim 83 wherein said a plurality of ultrasound- producing devices have at least one different operating characteristic 85 The method of claim 84, wherein said operating characteristic is selected from the group consisting of frequency, intensity, and coupling media 86 The method of claim 74, wherein said mechanical forces are applied by a device selected from the group consisting of a roller, a squeezer, a stretcher, a compressor, and a tensioner 87 The method of claim 86, wherein said tensioner collects said body fluid in a cavity formed therein 88 The method of claim 74, wherein said thermal forces are created by a source selected from the group consisting of electric, chemical, ultrasonic, and optical energy sources 89 The method of claim 74, wherein temperature sensitive polymers are used to extract body fluids 90 The method of claim 72, wherein said step of collecting said body fluid comprises using a collection method selected from the group consisting of absorption, adsorption, phase separation, mechanical, electrical, chemically induced, capillary forces, and a combination thereof 91 The method of claim 90, wherein said absorption collection method comprises collecting said body fluid into a gel 92 The method of claim 91, wherein said gel contains a captive enzyme 93 The method of claim 90, wherein said phase separation method comprises isolating said body fluid with an appropriate density immiscible fluid 94 The method of claim 93, further comprising collecting said body fluid into a conical chamber 95 The method of claim 90 wherein a hydrophobic coating is applied to said skin prior to said step of extracting a body fluid from said area of skin 96 The method of claim 75, wherein said body fluid is collected from said hydrophobic coating 97 The method of claim 90, wherein said mechanical collection method comprises applying a force selected from the group consisting of vacuum, pressure, and acoustic forces 98 The method of claim 90, wherein said electrical collection method comprises moving a charged object from said skin to a collecting compartment using electrical forces 99 The method of claim 90, wherein said chemical collection method comprises applying a hydrophilic gel to collect body fluids 100 The method of claim 90, wherein said capillary collection method comprises filling at least one capillary with a plurality of fibers, and collecting said body fluid in said at least one capillary 101 The method of claim 72, wherein said step of sensing the presence of at least one analyte comprises applying a sensing method selected from the group consisting of electrochemical, optical, acoustical, biological, enzymatic technology, and combinations thereof 102 The method of claim 72, wherein living cells are used to sense a concentration of an analyte in body fluid 103 The method of claim 72, further comprising the step of providing an output for a user interface comprises providing an alarm that indicates an abnormal analyte concentration 104 The method of claim 72, further comprising the step of providing an output for a user interface comprises providing an trend information 105 The method of claim 72, further comprising the step of providing history information. 106 The method of claim 72, wherein said user output is downloadable 107 A system for extraction and analysis of at least one analyte in a body fluid comprising a transducer for increasing the permeability of an area of skin, an extraction device for extracting interstitial fluid from said area of skin, a collection device for collecting said extracted interstitial fluid, and a sensing device for sensing the presence of at least one analyte in said extracted interstitial fluid 108 The system of claim 107, further comprising a microcontroller for controlling at least one of said transducer, said extraction device, said collection device, and said sensing device 109 The system of claim 107, further comprising a user output device 1 10 The system of claim 108, further comprising a microcontroller for controlling said user output device 1 1 1 The system of claim 107, wherein said transducer comprises an ultrasonic transducer 1 12 The system of claim 107, wherein said extraction device is a device that produces a force selected from the group consisting of physical forces, chemical forces, biological forces, vacuum pressure, electrical forces, osmotic forces, diffusion forces, electro-magnetic forces, ultrasound forces, cavitation forces, mechanical forces, thermal forces, capillary forces, fluid circulation across the skin, electro-acoustic forces, magnetic forces, magneto-hydrodynamic forces, acoustic forces, convective dispersion, photo acoustic forces, by rinsing body fluid off skin, and any combination thereof 1 13 The system of claim 107, wherein said collection device is a device that uses a collection method selected from the group consisting of absorption, adsorption, phase separation, mechanical, electrical, chemically induced, and a combination thereof 1 14 The system of claim 107, wherein said sensing device is a device that senses the presence of an analyte by a sensing method selected from the group consisting of electrochemical, optical, acoustical, biological, enzymatic technology, and combinations thereof 1 15 The system of claim 109, wherein said user output device provides information selected from the group consisting of trend information, history information, operating information, and combinations thereof 1 16 The system of claim 1 15, wherein information from said user output device is downloadable to a computer 1 17 A method for blood glucose determination comprising increasing a permeability of an area of skin, extracting interstitial fluid from said area of skin, collecting said interstitial fluid in a gel, said gel containing at least one glucose sensitive reagent that changes at least one characteristic of said gel when glucose is present, and monitoring a change in said at least one characteristic of said gel 1 18 A system for blood glucose determination comprising a transducer for increasing the permeability of an area of skin, an extraction device for extracting interstitial fluid from said area of skin, a collection device for collecting said extracted interstitial fluid, a gel in said collection device, at least one glucose sensitive reagent that changes at least one characteristic of said gel when glucose is present, and a monitoring device for monitoring a change in said at least one characteristic of said gel 1 19 The system of claim 1 18, wherein the at least one glucose sensitive reagent is in said gel 120 A drug delivery patch apparatus, comprising a transducer for applying ultrasound to a membrane, a power source coupled to said transducer, a plurality of drug molecules between said transducer and said biological membrane, an attaching device for attaching said drug molecules and said transducer to said membrane, and a user interface that interacts with said transducer, said power source and said drug molecules 121 The drug delivery patch apparatus of claim 120, further comprising drive electronics coupled to said transducer, said drive electronics enables said transducer to apply ultrasound 122 The drug delivery patch apparatus of claim 120, wherein said membrane is skin 123 The drug delivery patch apparatus of claim 120, wherein said drug molecules and said attaching device are combined 124 The drug delivery patch apparatus of claim 120, wherein said drug molecules are suspended in the group consisting of a liquid, a gel, and a solid matrix 125 The drug delivery patch apparatus of claim 120, wherein said power source is coupled to said transducer via hardwire 126 The drug delivery patch apparatus of claim 120, wherein said power source is coupled to said transducer via telemetry 127 The drug delivery patch apparatus of claim 120, wherein said transducer comprises acoustic elements, such that said acoustic elements are swept in a temporal nature as said transducer applies ultrasound 128 The drug delivery patch apparatus of claim 120, wherein said attaching device includes a band for attaching to said membrane 129 The drug delivery patch apparatus of claim 120, further comprising a feedback mechanism coupled to said drug molecules and said user interface for monitoring the amount of said drug molecules 130 The drug delivery patch apparatus of claim 120, further comprising a feedback mechanism coupled to said transducer and said user interface for monitoring the amount of ultrasound applied to said membrane 131 The drug delivery patch apparatus of claim 120, wherein said user interface is coupled to said transducer, said power source and said drug molecules by telemetry 132 The drug delivery patch apparatus of claim 120, wherein said user interface is coupled to said transducer, said power source and said drug molecules by an infrared device 133 The drug delivery patch apparatus of claim 120, wherein said user interface is coupled to said transducer, said power source and said drug molecules by fiber optics 134 The drug delivery patch apparatus of claim 120, wherein said transducer is configured as a cylinder. 135 The drug delivery patch apparatus of claim 120, wherein said transducer is configured as a hollow cylinder 136 The drug delivery patch apparatus of claim 120, wherein said transducer is a hemispherical configuration 137 The drug delivery patch apparatus of claim 120, wherein said transducer is a conical configuration 138 The drug delivery patch apparatus of claim 120, wherein said transducer is a planar configuration 139 The drug delivery patch apparatus of claim 120, wherein said transducer is a rectangular configuration 140 The drug delivery patch apparatus of claim 120, further comprising a device for creating a driving force that operates in conjunction with said transducer, said driving force selected from the group consisting of physical forces, chemical forces, biological forces, vacuum pressure, electrical forces, osmotic forces, diffusion forces, electromagnetic forces, ultrasound forces, cavitation forces, mechanical forces, thermal forces, capillary forces, fluid circulation across the skin, electro-acoustic forces, magnetic forces, magneto-hydrodynamic forces, acoustic forces, convective dispersion, photo-acoustic forces, by rinsing body fluid off skin, and any combination thereof 141 The drug delivery patch apparatus of claim 120, further comprising a limiting step membrane between said membrane and said drug molecules 142 The drug delivery patch apparatus of claim 120, wherein said membrane is pretreated by a force. 143. The drug delivery patch apparatus of claim 142, wherein said force is selected from the group consisting of physical forces, chemical forces, biological forces, vacuum pressure, electrical forces, osmotic forces, diffusion forces, electromagnetic forces, ultrasound forces, cavitation forces, mechanical forces, thermal forces, capillary forces, fluid circulation across the skin, electro-acoustic forces, magnetic forces, magneto-hydrodynamic forces, acoustic forces, convective dispersion, photo-acoustic forces, by rinsing body fluid off skin, and any combination thereof. 144. A method for delivering a drug through a membrane, the method comprising the steps of supplying power to a transducer that applies ultrasound, delivering drug molecules through said membrane by applying ultrasound to said drug molecules and having an attaching device for attaching at least said drug molecules and said transducer to said membrane 145. The method of claim 144, further comprising the step of pre-treating said membrane by applying a force prior to said delivering step, said force selected from the group consisting of physical forces, chemical forces, biological forces, vacuum pressure, electrical forces, osmotic forces, diffusion forces, electromagnetic forces, ultrasound forces, cavitation forces, mechanical forces, thermal forces, capillary forces, fluid circulation across the skin, electro- acoustic forces, magnetic forces, magneto-hydrodynamic forces, acoustic forces, convective dispersion, photo-acoustic forces, by rinsing body fluid off skin, and any combination thereof 146 The method of claim 144, wherein said delivering step includes applying a driving force in conjunction with said ultrasound from said transducer, said driving force selected from the group consisting of physical forces, chemical forces, biological forces, vacuum pressure, electrical forces, osmotic forces, diffusion forces, electromagnetic forces, ultrasound forces, cavitation forces, mechanical forces, thermal forces, capillary forces, fluid circulation across the skin, electro-acoustic forces, magnetic forces, magneto-hydrodynamic forces, acoustic forces, convective dispersion, photo-acoustic forces, by rinsing body fluid off skin, and any combination thereof. 147. A method for transdermal vaccination comprising increasing permeability of an area of skin; providing a vaccine on said area of skin; delivering said vaccine to at least one skin cell 148 The method of claim 147, wherein said step of increasing a permeability of an area of skin comprises applying ultrasound to said area of skin 149 The method of claim 147, wherein said step of providing a vaccine on said area of skin comprises providing a patch containing said vaccine on said area of skin. 150 The method of claim 147, wherein said step of providing a vaccine on said area of skin comprises providing a gel containing said vaccine on said area of skin. 151 . The method of claim 147, wherein said step of providing a vaccine on said area of skin comprises providing a liquid containing said vaccine on said area of skin. 152. The method of claim 147, wherein said step of delivering said vaccine to at least one skin cell comprises diffusing said vaccine to said at least one skin cell. 153. The method of claim 147, wherein said steps of increasing a permeability of a area of skin and providing a vaccine on said area of skin are simultaneous. 154. The method of claim 147, wherein said vaccine is selected from the group consisting of a peptide, a protein, DNA, an allergen, and other antigens. 155. The method of claim 147, wherein said at least one skin cell is selected from the group consisting of Langerhans cells, dendric cells, and keratinocytes. 156. The method of claim 147, wherein said step of delivery said vaccine to said immune cell comprises applying a driving force selected from the group consisting of physical forces, chemical forces, biological forces, vacuum, electrical forces, osmotic forces, diffusion forces, electro-magnetic forces, ultrasound forces, cavitation forces, mechanical forces, thermal forces, capillary forces, fluid circulation across the skin, electro-acoustic forces, magnetic forces, magneto- hydrodynamic forces, acoustic forces, convective dispersion, photo acoustic forces, and any combination thereof 157. A method for transdermal vaccination and immunization, comprising: applying ultrasound to irritate a area of skin; and providing a vaccine to said area of skin.

Inventors: ELSTROM TUAN A, KELLOGG SCOTT C, KOST JOSEPH, MITRAGOTRI SAMIR S, WARNER NICK, ELSTROM, TUAN, A., KELLOGG, SCOTT, C., KOST, JOSEPH, MITRAGOTRI, SAMIR, S., WARNER, NICK
Applicants: ELSTROM TUAN A, KELLOGG SCOTT C, KOST JOSEPH, MITRAGOTRI SAMIR S, SONTRA MEDICAL INC, WARNER NICK, ELSTROM, TUAN, A., KELLOGG, SCOTT, C., KOST, JOSEPH, MITRAGOTRI, SAMIR, S., SONTRA MEDICAL, INC., WARNER, NICK
Application year: 1999
Main classification: A61B17
IPC codes: A61B5, A61B18, A61K9, A61K47, A61K49, A61M35, A61M37, A61N1, G01N33