PROCESS FOR OBTAINING HMG-CoA REDUCTASE INHIBITORS OF HIGH PURITY

Abstract: Lovastatin, pravastatin, simvastatin, mevastatin, atorvastatin, and derivatives and analogs thereof are known as HMG-CoA reductase inhibitors and are used as antihypercholesterolemic agents. The majority of them are produced by fermentation using microorganisms of different species identified as species belonging to Aspergillus, Monascus, Nocardia, Amycolatopsis, Mucor or Penicillium genus, some are obtained by treating the fermentation products using the method of chemical synthesis or they are the products of total chemical synthesis. The purity of the active ingredient is an important factor for manufacturing the safe and effective pharmaceutical, especially if the pharmaceutical product must be taken on a longer term basis in the treatment or prevention of high plasma cholesterol. The accumulation of the impurities from the pharmaceuticals of lower purity may cause many side effects during the medical treatment. The present invention relates to a new industrial process for the isolation of HMG-CoA reductase inhibitors using so-called displacement chromatography. Use of the invention enables to obtain HMG-CoA reductase inhibitors of high purity, with high yields, lower production costs and suitable ecological balance.

Description: Title of the invention Process for obtaining HMG-CoA reductase inhibitors of high purity Technical Field Lovastatin, pravastatin, simvastatin, mevasta.tin, atorvastatin and derivatives and analogs thereof are known as HMG-CoA reductase inhibitors and are used as antihypercholesterolemic agents. The majority of them are produced by fermentation using microorganisms of different species identified as species belonging to Aspergillus , Monascus , Nocardia , Amycolatopsis , Mucor or Penicillium genus, some are obtained by treating the fermentation products using the method of chemical synthesis or they are the products of total chemical synthesis. The purity of the active ingredient is an important factor for manufacturing the safe and effective pharmaceutical, especially if the pharmaceutical product must be taken on a longer term basis in the treatment or prevention of high plasma cholesterol. The accumulation of the impurities from the pharmaceuticals of lower purity may cause many side effects during the medical treatment. The present invention relates to a new industrial process for the isolation of HMG-CoA reductase inhibitors using so-called displacement chromatography . Use of the invention enables to obtain HMG-CoA reductase inhibitors of high purity, with high yields, lower production costs and suitable ecological balance. Prior Art The processes for the isolation and purification of antihypercholesterolemic agents disclosed in the earlier patents include a variety of combinations of extraction, chromatography, lactonisation and crystallisation methods. The purity of the final product obtained by these procedures comply with the USP standards but the yields of the desired product are relatively low. In addition, they require both large amounts of organic solvents and the large equipment suited for these quantities. The isolation process disclosed in WO 92/16276 provides the solution for obtaining HMG-CoA reductase inhibitors of purity greater than 99.5% with the use of industrial HPLC (high performance liquid chromatography) equipment. According to WO 92/16276 the crude HMG-CoA reductase inhibitor, with a purity of > 85%, is dissolved in an organic solvent or in a solution of organic solvent and water. The mixture is then buffered to a pH between 2 and 9 and placed on the HPLC column. After the HMG-CoA reductase inhibitor peak of interest is collected, a portion of solvent is removed and the water is added or alternatively two-thirds of the solvent mixture are removed and the HMG-CoA reductase inhibitor is crystallised. At the end, the purity of the product obtained by this process is at least 99.5% with the yield of around 90%. The method disclosed in WO 92/16276 enables obtaining of HMG-CoA reductase inhibitors of high purity, with relatively high yields, the disadvantage of the method over the conventional chromatography columns are relatively small quantities of the substance loaded per HPLC column. Small samples to be fed into the column are also related with increased number of repetitions of the isolation operations in order to obtain sufficient quantities of the desired substance, and consequently large amount of the solvents used resulting in higher production costs. Displacement chromatography method, the basis of the present invention, does not substantially differ from previously used chromatography methods. Displacement chromatography is based on competition of the components of the sample fed into the column for active sites on the stationary phase. Individual components of the sample displace one another like a train, the displacer, having the very high affinity for the stationary phase and travelling behind the fed sample along the column, drives the separation of the sample components into one-compartment zones which move at the same velocity as the displacer. Concentrating of individual components is carried out simultaneously with the purification. The principle of displacement chromatography method is relatively old as it has been known since 1943 but it was introduced into practice as late as 1981 because of the lack of efficient columns (Cs. Horvath et al., J. Chromatogr., 215 (1981) 295; J. Chromatogr., 330 (1985) 1; J. Chromatogr., 440 (1988) 157). These papers, introduced herein by way of reference, describe the analytic and preparative separation and purification of biologically active peptides and polymyxin antibiotics (polypeptides) using reversed-phase high performance liquid chromatography columns in the displacement mode. For polymyxins octadecyl silica gel columns 250 x 4.6 mm, particle size 5μm, 10% acetonitrile in water as the mobile phase and different tetraalkylammonium halogenides as the displacer were used. In recent investigations in the field of displacement chromatography (S.M. Cramer et al., Enzyme Microb. Technol., 11 (1989) 74; Prep. Chromatogr., 1 (1988) 29; J. Chromatogr., 394 (1987) 305; J. Chromatogr., 439 (1988) 341; J. Chromatogr., 454 (1988) 1 (theoretic optimisation)); A. Felinger et al., J. Chromatogr., 609 (1992) 35 (theoretic optimisation) , all papers being introduced herein by way of reference) similar columns were used; the mobile phase was methanol in the phosphate buffer, the displacer was 2- (2-t-butoxyethoxy) ethanol (BEE) in acetonitrile and sodium acetate. Different peptides, proteins and cephalosporin C antibiotic were used as the samples. US Pat. No. 5,043,432 (27.08.1991) and EP 416.416, respectively, describe the method for purifying certain low molecular (below 1000 daltons) peptides (in particular, tuftsin and synthetic derivatives thereof) with displacement ion-exchange chromatography where the stationary phase used is cationic-exchange resin, the transporter solvent is water or dilute solutions of a variety of strong acids, and the displacer used is triethylenetetraammonuim salt in different concentrations. In US patent application 08/875,422, yet unpublished, the use of displacement chromatography for the isolation and purification of vancomycin is described. Technical Solution It is sometimes difficult to obtain the active substance of high purity in a large scale as many technologies applicable to a laboratory scale are not sufficiently economical in large scale production operations to justify use thereof or do not meet the environmental criteria. The above facts compel the industry to search for new techno- logies that will provide both the high-quality product and the economically and ecologically acceptable production. The present invention has solved the drawbacks of the processes known from the older patents and other literature as it enables to obtain the pure HMG-CoA reductase inhibitors and, additionally, the purifying process per se is not time-consuming providing high yields, using small amounts of solvents. The process is nature friendly; in addition, it is not demanding in terms of space and energy thus enabling an economical large scale production. Description of the invention The present invention provides a process for the purification of HMG-CoA reductase inhibitors employing displacement chromatography. That is, at least one of the steps in the process of the purification of crude HMG-CoA reductase inhibitor includes displacement chromatography. The HMG-CoA reductase inhibitor to be purified is, for example, selected from the group consisting of mevastatin, pravastatin, lovastatin, simvastatin, fluvastatin and atorvastatin. The selected inhibitor may be in the lactone form or in the form of the acid or the salt thereof for being purified by means of displacement chromatography. The displacement chromatography being characteristic for the process of the present invention preferably includes the following steps: a) conditioning a chromatography column with an appropriate mobile phase, b) feeding the crude HMG-CoA reductase inhibitor dissolved in the mobile phase, c) introducing the displacer for displacing the HMG-CoA reductase inhibitor from the column, and d) obtaining the purified HMG-CoA reductase inhibitor. The purified HMG-CoA reductase inhibitor is preferably obtained by dl) collecting the fractions and d2) analyzing the fractions with analytical HPLC and pooling the fractions depending on the quality of purity. After the purified HMG-CoA reductase inhibitor has been obtained, the chromatography column may be regenerated by washing of the column with alcohol/water mixture to elute the displacer. HMG-CoA reductase inhibitors obtained in the herein- described manner are then isolated from the mobile phase according to the methods already known from the state of prior art, for example by lyophilisation or, prefarably, by crystallization to obtain the lactone form, the acid form or the salt form (preferably alkaline or earth alkaline salts) thereof. The fractions containing a considerable percentage of HMG- CoA reductase inhibitors, in addition to impurities, may be re-subjected to the process resulting in the total yield exceeding 95%. The stationary phase used is a reverse phase where natural (silica gel with alkyl chains of a different length) or synthetic (C-18 or C-8 organic) stationary phases are suitable. Preferably, a synthetic cross-linked polymer matrix of styrene and divinylbenzene is used. The particle size of the stationary phase is suitably from 3 to 20 μm, preferably between 7 and 15 μm. The mobile phase used is preferably selected from water, acetonitrile/water solution and aqueous solutions of lower (preferably Cι-C ) alcohols, buffered dilute solutions of organic, halogenated organic or inorganic acids, e.g. formic, acetic, propionic, hydrochloric, boric, phosphoric, carbonic or suphuric acids with cations of alkaline metals, with ammonia or with amines. Water and aqueous solutions with acetonitrile and especially with methanol or ethanol are particularly preferred, and the content of the organic solvent in the aqueous solutions preferably is 80% or below, more preferably 45% or below and particularly 30% or below. Since toxic methanol in the mobile phase may be replaced by less toxic ethanol, or may be at least partially replaced by water with good results, removal of waste solvents is simpler, therefore, the present invention is a marked improvement compared to the state of prior art judging from the ecological aspect. The pH of the mobile phase used is preferably between 4.5 and 10.5, more preferably between 6.5 and 8, and particularly around 7. The flow rate of the mobile phase through the column is suitably adjusted to lie between 1.5 and 30 ml/ (min cm 2 ), preferably between 3 and 15 ml/ (min cm 2 ). At the time when the displacer is introduced into the chromatography column by being mixed with the mobile phase, the flow rate is preferably adjusted to lie between 1.5 and 15 ml/ (min cm 2 ) and particularly between 3 and 10 ml/ (min cm 2 ), because higher flow rates cause the dilution of the samples to be collected, and also the separation becomes worse. The displacer suitably is a compound having an amphiphilic structure, such as surfactants, detergents and the like. Examples of the displacer are long chain alcohols, long chain carboxylic acids, long chain alkyl ammonium salts, aromatic dicarboxylic acid esters, oxo- and dioxo- alcohols, polyalkylene polyglycol ethers such as diethylene glycol mono- (or di-) alkylethers, polyaryl or polyalkylene polyaryl ethers such as Triton ® X-100, etc. The aforementioned "long chain" means an alkyl chain having at least a C 4 -chain, preferably at least a C 10 -chain and more preferably at least a C 14 -chain or longer. The concentration of the displacer in the mobile phase is suitably adjusted to be from 1 to 35%, preferably from 2 to 20% and particularly from 7 to 14%. In the preferred embodiment of controlling the quality of purity in the individual fractions eluted from the chromatography column, an analytical HPLC method directed to the HMG-CoA reductase inhibitors to be analyzed may be carried out as described in the following. The sample to be analysed is diluted 100 times with the mobile phase containing 20 mM aqueous NH 4 HCO 3 solution with acetonitrile (the proportion of acetonitrile is adjusted such that the retention factor of the analyte is between 5 and 10) . 10 μl of this sample is placed on Hypersil ODS column (Hypersil, the United Kingdom, particle size 3μm, column size 50 x 4.6 mm) for high performance liquid chromatography. The column is washed with the mobile phase at the flow rate of 2 ml/min. Absorbance is measured at 235 nm. HPLC purity of the sample is calculated from the ratio between the areas of individual peaks in the chromatogram. After completed chromatography the stationary phase is preferably regenerated, for example using the mobile phase with 20 to 100% aqueous solution of lower alcohol. The invention is illustrated but in no way limited by the following examples. EXAMPLES Example 1 Crude sodium salt of pravastatin (1.0 g, HPLC purity 88%, assay 85%) was dissolved in 10 ml of the mobile phase A (distilled water), pH was adjusted to 7 with 0.2M aqueous NaOH solution and filtered. The column was equilibrated with mobile phase A. The sample obtained in the above- described manner was fed onto the Grom-Sil 120-ODS HE column (Grom Analytic + HPLC GmbH, Germany) , particle size 11 μm, column size 250 x 10 mm. The column was washed with the mobile phase B containing 7% of diethyleneglycol monobutylether in mobile phase A at the flow rate of 4.5 ml/min. Absorbance was measured at 260 nm, and the 0.5 ml fractions were collected with an initial increase in the absorbance. When the signal decreased the column was washed with 25 ml of 70% methanol. The obtained fractions were analyzed by the herein above-described HPLC analytical method. The fractions with a purity > 99.5% were pooled. In the pooled fractions (7 ml) the HPLC purity was 99.8%. Example 2 Crude sodium salt of pravastatin (0.4 g, HPLC purity 88%, assay 85%) was dissolved in 5 ml of the mobile phase A (distilled water), pH was adjusted to 7 with 0.2M aqueous NaOH solution and filtered. The column was equilibrated with mobile phase A. The sample obtained in the above- described manner was fed onto the Kromasil 100 C-18 column (EKA Chemicals AB, Sweden) , particle size 10 μm, column size 200 x 10 mm. The column was washed with the mobile phase B containing 7% of Triton X-100 in mobile phase A at the flow rate of 1 ml/min. Absorbance was measured at 260 nm, and the 0.5 ml fractions were collected with an initial increase in the absorbance. The obtained fractions were analysed by the above described HPLC analytical method. The fractions with a purity > 99.5% were pooled. In the pooled fractions (3 ml) the HPLC purity was 99.7%. Example 3 0.6 g of the crude sodium salt of pravastatin was dissolved in 5 ml of distilled water. The protocol described in Example 1 was used with the exception of the mobile phase used (30% aqueous methanol solution) and the pooled fractions with a HPLC purity of 99.8% were obtained. Example 4 The method described in Example 3 was repeated wherein the concentration of the displacer in the mobile phase was 14%. In the fractions pooled, according to the criterion described in Example 1, HPLC purity was 99.8%. Example 5 Pravastatin lacton (0.4g, HPLC purity 85%) was dissolved in 33 ml of the mobil phase A containing 45% methanol. The column was equilibrated with mobile phase A. The sample obtained in the above-described manner was fed onto the Grom-Sil 120-ODS HE column (Grom Analytic + HPLC GmbH, Germany) , particle size 11 μm, column size 250 x 10 mm. The column was washed with the mobile phase B containing 2% of diethyleneglycoldibutylether in mobile phase A at the flow rate of 4.5 ml/min. Absorbance was measured at 260 nm, and the 1ml fractions were collected with an initial increase in the absorbance. When the signal decreased the column was washed with 25 ml of 70% methanol. The fractions with a purity > 99.5% were pooled. In the pooled fractions the HPLC purity was 99.7%. Example 6 Pravastatin lacton (0.3g, HPLC purity 85%) was dissolved in 80 ml of the mobil phase A containing 30% methanol. The column was equilibrated with mobile phase A. The sample obtained in the above-described manner was fed onto the Licrosphere RP 18 column, particle size 12 μm, column size 200 x 10 mm. The column was washed with the mobile phase B containing 5% of diethyleneglycolmono-n-hexylether in mobile phase A at the flow rate of 4.5 ml/min. Absorbance was measured at 235 nm, and the 1ml fractions were collected with an initial increase in the absorbance. When the signal decreased the column was washed with 25 ml of 90% methanol. The obtained fractions were analysed by the above described HPLC analytical method. The fractions with a purity > 99.5% were pooled. In the " pooled fractions the HPLC purity was 99.8%. Example 7 Pravastatin lacton (0.3g, HPLC purity 85%) was dissolved in 25 ml of the mobil phase A containing 35% acetonitrile. The column was equilibrated with mobile phase A. The sample obtained in the above-described manner was fed onto the Licrosphere RP 18 column, particle size 12 μm, column size 200 x 10 mm. The column was washed with the mobile phase B containing 1% of diethyleneglycoldibutylether in mobile phase A at the flow rate of .5 ml/min. Absorbance was measured at 235 nm, and the 1ml fractions were collected with an initial increase in the absorbance. When the signal decreased the column was washed with 25 ml of 90% methanol. The obtained fractions were analysed by the above described HPLC analytical method. The fractions with a purity > 99.5% were pooled. In the pooled fractions the HPLC purity was 99.8%. Example 8 The method described in Example 7 was repeated wherein the mobile phase B was 0.85% diethylphthalat in the mobile phase A. The fractions with a purity > 99.5% were pooled. In the pooled fractions the HPLC purity was 99.8%. Example 9 Simvastatin lacton (0.42g, HPLC purity 87%) was dissolved in 6 ml of the 66% acetonitrile and hydrolysed with 1.2mmol of sodium hydroxide. Acetonitrile was removed and pH was adjusted to 7 with diluted H 3 P0 4 . The column was equilibrated with mobile phase A containing 14% of methanol. The sample obtained in the above-described manner was fed onto the Grom-Sil 120-ODS HE column (Grom Analytic + HPLC GmbH, Germany) , particle size 11 μm, column size 250 x 10 mm. The column was washed with the mobile phase B containing 6.7% of diethyleneglycolmono-n- hexylether in mobile phase A at the flow rate of 4.5 ml/min. Absorbance was measured at 260 nm, and the 0.5ml fractions were collected with an initial increase in the absorbance. When the signal decreased the column was washed with 25 ml of methanol. The fractions with a purity > 99.5% were pooled. In the pooled fractions the HPLC purity was 99.8%. Example 10 Simvastatin lacton (0.5g, HPLC purity 87 %) was dissolved in 20 ml of the mobile phase containing 70% of methanol. The column was equilibrated with mobile phase A. The sample obtained in the above-described manner was fed onto the Grom-Sil 120-ODS HE column (Grom Analytic + HPLC GmbH, Germany) , particle size 11 μm, column size 250 x 10 mm. The column was washed with the mobile phase B containing 3% of decanoic acid in mobile phase A at the flow rate of 4.5 ml/min. Absorbance was measured at 260 nm, and the 0.75 ml fractions were collected with an initial increase in the absorbance. When the signal decreased the column was washed with 25 ml of methanol. The obtained fractions were analyzed by the herein above described method. The fractions with a purity > 99.5% were pooled. In the pooled fractions the HPLC purity was 99.7%. Example 11 Simvastatin lacton (0.5 g, HPLC purity 87%) was dissolved in 20 ml of the mobile phase containing of 60% acetonitrile. The column was equilibrated with mobile phase A . The sample obtained in the above-described manner was fed onto the Grom-Sil 120-ODS HE column (GrortT Analytic + HPLC GmbH, Germany) , particle size 11 μm, column size 250 x 10 mm. The column was washed with the mobile phase B containing 2% of tetrakis (decyl) amonium bromide in mobile phase A at the flow rate of 4.5 ml/min. Absorbance was measured at 260 nm, and the 1ml fractions were collected with an initial increase in the absorbance. When the signal decreased the column was washed with 25 ml of methanol. The fractions with a purity > 99.5% were pooled. In the pooled fractions the HPLC purity was 99.8%. Example 12 Lovastatin lacton (0.5g, HPLC purity 87%) was dissolved in 60 ml of the 75% methanol. The column was equilibrated with mobile phase A containing 70% of methanol. The sample obtained in the above-described manner was fed onto the Grom-Sil 120-ODS HE column (Grom Analytic + HPLC GmbH, Germany) , particle size 11 μm, column size 250 x 10 mm. The column was washed with the mobile phase B containing 70% of methanol and 4.5% of decanoic acid in mobile phase A at the flow rate of 6 ml/min. Absorbance was measured at 260 nm, and the 1ml fractions were collected with an initial increase in the absorbance. When the signal decreased the column was washed with 25 ml of methanol. The obtained fractions were analysed by the above described HPLC analytical method. The fractions with a purity > 99.5% were pooled. In the pooled fractions the HPLC purity was 99.9%. Example 13 Lovastatin lacton (0.42g, HPLC purity 87 %) was dissolved in 8 ml of the 50% acetonitrile and hydrolysed with 1.5 mmol of sodium hydroxide. Acetonitrile was removed and pH was adjusted to 7 with diluted H 3 P0 4 . The column was equilibrated with mobile phase A containing 14% of methanol. The sample obtained in the above-described manner was fed onto the Grom-Sil 120-ODS HE column (Grom Analytic + HPLC GmbH, Germany) , particle size 11 μm, column size 250 x 10 mm. The column was washed with the mobile phase B containing 6.7% of diethyleneglycolmono-n- hexylether in mobile phase A at the flow rate of 1 ml/min. Absorbance was measured at 260 nm, and the 0.25 ml fractions were collected with an initial increase in the absorbance. When the signal decreased the column was washed with 25 ml of methanol. The obtained fractions were analysed by the method described in example 9. The fractions with a purity > 99.5% were pooled. In the pooled fractions the HPLC purity was 99.8%. Example 14 Mevastatin lacton (0.5g, HPLC purity 85%) was dissolved in 150 ml of the mobile phase A containing 70% of methanol. The column was equilibrated with mobile phase A. The sample obtained in the above-described manner was fed onto the Grom-Sil 120-ODS HE column (Grom Analytic + HPLC GmbH, Germany) , particle size 11 μm, column size 250 x 10 mm. The column was washed with the mobile phase B containing 4.5% of decanoic acid in mobile phase A at the flow rate of 6 ml/min. Absorbance was measured at 260 nm, and the 1 ml fractions were collected with an initial increase in the absorbance. When the signal decreased the column was washed with 25 ml of methanol. The obtained fractions were analysed by the above described HPLC analytical method. The fractions with a purity > 99.5% were pooled. In the pooled fractions the HPLC purity was 99.8%.

Claims: Claims 1 . A process for obtaining HMG-CoA reductase inhibitors, characterised in that one of the steps in the process of the purification of crude HMG-CoA reductase inhibitors includes displacement chromatography. 2. A process according to claim 1, characterised in that the HMG-CoA reductase inhibitor is selected from the group consisting of mevastatin, pravastatin, lovastatin, simvastatin, fluvastatin and atorvastatin. 3. A process according to claim 1 or 2, characterised in that the HMG-CoA reductase inhibitor is in the lactone form or in the form of the acid or the salt thereof. 4. A process according any one of claims 1 to 3, characterised in that the displacement chromatography includes the following steps: a) conditioning a chromatography column with a mobile phase, b) feeding HMG-CoA reductase inhibitor dissolved in the mobile phase, c) introducing the displacer for displacing the HMG-CoA reductase inhibitor from the column, and d) obtaining the purified HMG-CoA reductase inhibitor. 5. A process according to claim 4, characterised in that the purified HMG-CoA reductase inhibitor is obtained by dl) collecting the fractions, and d2) analyzing the fractions with analytical HPLC and pooling the fractions depending on the quality of purity. 6. A process according to claim 4 or 5, characterised in that the displacement chromatography further includes the subsequent step of: e) regenerating the chromatography column by washing the column with alcohol/water mixture to elute the displacer. 7. A process according to claim 4, characterised in that the mobile phase is selected from the group of solvents consisting of water, acetonitrile/water solutions or aqueous solutions of lower alcohols, as well as bufferred dilute solutions of organic, halogenated organic or inorganic acids with alkaline metal cations, .with ammonia or with amines. 8. A process according to claim 7, characterised in that the mobile phase is any one of water, an acetonitrile/water solution or an aqueous solution of lower alcohols. 9. A process according to claim 4, characterised in that the pH of the mobile phase used is between 4.5 and 10.5. 10. A process according to claim 9, characterised in that the pH of the mobile phase used is between 6.5 and 8. 11. A process according to claim 10, characterised in that the pH of the mobile phase used is 7. 12. A process according to claim 4, characterised in that the flow rate of the mobile phase through the chromatographic column is between 1.5 and 30 ml/ (min cm 2 ). 13. A process according to claim 4, characterised in that the flow rate of the mobile phase/displacer mixture through the chromatographic column is between 3 and 15 ml/ (min cm 2 ) . 14. A process according to claim 6, characterised in that the stationary phase is regenerated with 20 to 100% aqueous solution of lower alcohols after completed chromatography. 15. A process according to claim 4, characterised in that the stationary phase is a reverse phase. 16. A process according to claim 15, characterised in that the stationary phase is a natural reverse phase such as silica gel with alkyl chains of different lengths. 17. A process according to claim 15, characterised in that the stationary phase is either C-18 or C-8. 18. A process according to claim 15, characterised in that the stationary phase is a synthetic cross-linked polymer matrix. 19. A process according to claim 18, characterised in that the cross-linked polymer matrix is a copolymer of styrene and divinylbenzene. 20. A process according to claim 4, characterised in that the particle size of the stationary phase is between 3 and 20 μm. 21. A process according to claim 20, characterised in that the particle size of the stationary phase is between 7 and 15 μm. 22. A process according to claim 4, characterised in that the displacer is selected from the group consisting of long chain alcohols, long chain carboxylic acids, long chain alkyl ammonium salts, aromatic dicarboxylic acid esters, oxo- and dioxo-alcohols, polyalkylene polyglycol ethers and polyaryl or polyalkylene polyaryl ethers. 23. A process according to claim 4, characterised in that the concentration of the displacer in the mobile phase is 5 between 1 and 35%. 24. A process according to claim 23, characterised in that the concentration of the displacer in the mobile phase is between 2 and 20%. 25. The use of a process according to any one of claims 1 10 to 24 for producing a HMG-CoA reductase inhibitor with a HPLC purity exceeding 99.7%. 26. HMG-CoA reductase inhibitor with a HPLC purity exceeding 99.7% obtained by purifying crude HMG-CoA reductase inhibitor by means of a purification process 15. which includes displacement chromatography. 27. The substance according to claim 26, characterized in that the HMG-CoA reductase inhibitor is selected from the group consisting of lovastatin, simvastatin, pravastatin, atorvastatin, mevastatin and fluvastatin. 20 28. The substance according to claim 27, characterized in that the selected HMG-CoA reductase inhibitor is lovastatin, simvastatin or pravastatin. 29. The substance according to claim 26, characterized in that the selected HMG-CoA reductase inhibitor is in the 25 lactone form or in the form of the acid or the salt thereof.

Inventors: BASTARDA ANDREJ, GRAHEK ROK, MILIVOJEVIC DUSAN, BASTARDA, ANDREJ, GRAHEK, ROK, MILIVOJEVIC, DUSAN
Applicants: BASTARDA ANDREJ, GRAHEK ROK, LEK TOVARNA FARMACEVTSKIH, MILIVOJEVIC DUSAN, BASTARDA, ANDREJ, GRAHEK, ROK, LEK PHARMACEUTICAL AND CHEMICAL COMPANY D.D., MILIVOJEVIC, DUSAN
Application year: 1999
Main classification: C07D309
IPC codes: A61K31, A61P3, B01D15, C07C67, C07C69, C07D207, C07D209, C07D309, C07D405, C07K1, C12N9