NEISSERIA MENINGITIDIS ANTIGENS AND COMPOSITIONS

Abstract: The invention provides proteins from Neisseria meningitidis, including the amino acid sequences and the corresponding nucleotide sequences. The proteins are predicted to be useful antigens for vaccines and/or diagnostics.

Description: NEISSERIA MENINGITIDIS ANTIGENS AND COMPOSITIONS This application is a continuation-in-part of the following U.S. Provisional Patent applications, from each of which priority is claimed, and each of which is incorporated by reference in its entirety: 60/083,758 (filed May 1, 1998); 60/094,869 (filed July 31, 1998); 60/098,994 (filed September 2, 1998); 60/099,062 (filed September 2, 1998); 60/103,749 (filed October 9, 1998); 60/103,794 (filed October 9, 1998); 60/103,796 (filed October 9, 1998); and 60/121,528 (filed February 25, 1999). This invention relates to antigens from the bacterial species: Neisseria meningitidis and Neisseria gonorrhoeae. BACKGROUND Neisseria meningitidis is a non-motile, gram negative diplococcus human pathogen. It colonizes the pharynx, causing meningitis and, occasionally, septicaemia in the absence of meningitis. It is closely related to N gonorrhoea, although one feature that clearly differentiates meningococcus from gonococcus is the presence of a polysaccharide capsule that is present in all pathogenic meningococci. N meningitidis causes both endemic and epidemic disease. In the United States the attack rate is 0.6-1 per 100,000 persons per year, and it can be much greater during outbreaks, (see Lieberman et al. (1996) Safety and Immunogenicity of a Serogroups A/C Neisseria meningitidis Oligosaccharide-Protein Conjugate Vaccine in Young Children. JAMA 275(19):1499-1503; Schuchat et al (1997) Bacterial Meningitis in the United States in 1995. N Engl J Med 337(14):970-976). In developing countries, endemic disease rates are much higher and during epidemics incidence rates can reach 500 cases per 100,000 persons per year. Mortality is extremely high, at 10-20% in the United States, and much higher in developing countries. Following the introduction of the conjugate vaccine against Haemophilus influenzae, N meningitidis is the major cause of bacterial meningitis at all ages in the United States (Schuchat et al (1997) supra). Based on the organism's capsular polysaccharide, 12 serogroups of N. meningitidis have been identified. Group A is the pathogen most often implicated in epidemic disease in sub-Saharan Africa. Serogroups B and C are responsible for the vast majority of cases in the United States and in most developed countries. Serogroups W135 and Y are responsible for the rest of the cases in the United States and developed countries. The meningococcal vaccine currently in use is a tetravalent polysaccharide vaccine composed of serogroups A, C, Y and W135. Although efficacious in adolescents and adults, it induces a poor immune response and short duration of protection, and cannot be used in infants [eg. Morbidity and Mortality weekly report, Vol.46, No. RR-5 (1997)]. This is because polysaccharides are T-cell independent antigens that induce a weak immune response that cannot be boosted by repeated immunization. Following the success of the vaccination against H.influenzae, conjugate vaccines against serogroups A and C have been developed and are at the final stage of clinical testing (Zollinger WD "New and Improved Vaccines Against Meningococcal Disease". In: New Generation Vaccines, supra, pp. 469-488; Lieberman et al (1996) supra; Costantino et al (1992) Development and phase I clinical testing of a conjugate vaccine against meningococcus A and C. Vaccine 10:691-698). Meningococcus B (menB) remains a problem, however. This serotype currently is responsible for approximately 50% of total meningitis in the United States, Europe, and South America. The polysaccharide approach cannot be used because the menB capsular polysaccharide is a polymer of (2-8)-linked N-acetyl neuraminic acid that is also present in mammalian tissue. This results in tolerance to the antigen; indeed, if an immune response were elicited, it would be anti-self, and therefore undesirable. In order to avoid induction of autoimmunity and to induce a protective immune response, the capsular polysaccharide has, for instance, been chemically modified substituting the N-acetyl groups with N-propionyl groups, leaving the specific antigenicity unaltered (Romero & Outschoorn (1994) Current status of Meningococcal group B vaccine candidates: capsular or non-capsular? Clin MicrobiolRev 7(4):559-575). Alternative approaches to menB vaccines have used complex mixtures of outer membrane proteins (OMPs), containing either the OMPs alone, or OMPs enriched in porins, or deleted of the class 4 OMPs that are believed to induce antibodies that block bactericidal activity. This approach produces vaccines that are not well characterized. They are able to protect against the homologous strain, but are not effective at large where there are many antigenic variants of the outer membrane proteins. To overcome the antigenic variability, multivalent vaccines containing up to nine different porins have been constructed (eg. Poolman JT (1992) Development of a meningococcal vaccine. Infect. Agents Dis. 4:13-28). Additional proteins to be used in outer membrane vaccines have been the opa and ope proteins, but none of these approaches have been able to overcome the antigenic variability (eg. Ala'Aldeen & Borriello (1996) The meningococcal transferrin-binding proteins 1 and 2 are both surface exposed and generate bactericidal antibodies capable of killing homologous and heterologous strains. Vaccine 14(l):49-53). A certain amount of sequence data is available for meningococcal and gonoccocal genes and proteins (e.g. EP-A-0467714, WO96/29412), but this is by no means complete. Other men B proteins may include those listed in WO 97/28273, WO 96/29412, WO 95/03413, US 5,439,808, and US 5,879,686. The provision of further sequences could provide an opportunity to identify secreted or surface-exposed proteins that are presumed targets for the immune system and which are not antigenically variable. For instance, some of the identified proteins could be components of efficacious vaccines against meningococcus B, some could be components of vaccines against all meningococcal serotypes, and others could be components of vaccines against all pathogenic Neisseriae including Neisseria meningitidis or Neisseria gonorrhoeae. Those sequences specific to N meningitidis or N gonorrhoeae that are more highly conserved are further preferred sequences. It is thus an object of the invention is to provide Νeisserial DΝA sequences which encode proteins that are antigenic or immunogenic. BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 illustrates the products of protein expression and purification of the predicted ORF 919 as cloned and expressed in E. coli. Fig. 2 illustrates the products of protein expression and purification of the predicted ORF 279 as cloned and expressed in E. coli. Fig. 3 illustrates the products of protein expression and purification of the predicted ORF 576-1 as cloned and expressed in E. coli. Fig. 4 illustrates the products of protein expression and purification of the predicted ORF 519-1 as cloned and expressed in E. coli. Fig. 5 illustrates the products of protein expression and purification of the predicted ORF 121-1 as cloned and expressed in E. coli. Fig. 6 illustrates the products of protein expression and purification of the predicted ORF 128-1 as cloned and expressed in E. coli. Fig. 7 illustrates the products of protein expression and purification of the predicted ORF 206 as cloned and expressed in E. coli. Fig. 8 illustrates the products of protein expression and purification of the predicted ORF 287 as cloned and expressed in E. coli. Fig. 9 illustrates the products of protein expression and purification of the predicted ORF 406 as cloned and expressed in E. coli. Fig. 10 illustrates the hydrophilicity plot, antigenic index and AMPHI regions of the products of protein expression the predicted ORF 919 as cloned and expressed in E. coli. Fig. 11 illustrates the hydrophilicity plot, antigenic index and AMPHI regions of the products of protein expression the predicted ORF 279 as cloned and expressed in E. coli. Fig. 12 illustrates the hydrophilicity plot, antigenic index and AMPHI regions of the products of protein expression the predicted ORF 576-1 as cloned and expressed in E. coli. Fig. 13 illustrates the hydrophilicity plot, antigenic index and AMPHI regions of the products of protein expression the predicted ORF 519-1 as cloned and expressed in E. coli. Fig. 14 illustrates the hydrophilicity plot, antigenic index and AMPHI regions of the products of protein expression the predicted ORF 121-1 as cloned and expressed in E. coli. Fig. 15 illustrates the hydrophilicity plot, antigenic index and AMPHI regions of the products of protein expression the predicted ORF 128-1 as cloned and expressed inE. coli. Fig. 16 illustrates the hydrophilicity plot, antigenic index and AMPHI regions of the products of protein expression the predicted ORF 206 as cloned and expressed in E. coli. Fig. 17 illustrates the hydrophilicity plot, antigenic index and AMPHI regions of the products of protein expression the predicted ORF 287 as cloned and expressed in E. coli. Fig. 18 illustrates the hydrophilicity plot, antigenic index and AMPHI regions of the products of protein expression the predicted ORF 406 as cloned and expressed in E. coli. Fig. 19 shows an alignment comparison of amino acid sequences for ORF 225 for several strains of Neisseria. Dark shading indicates regions of homology, and gray shading indicates the conservation of amino acids with similar characteristics. The Figure demonstrates a high degree of conservation among the various strains, further confirming its utility as an antigen for both vaccines and diagnostics. Fig. 20 shows an alignment comparison of amino acid sequences for ORF 235 for several strains of Neisseria. Dark shading indicates regions of homology, and gray shading indicates the conservation of amino acids with similar characteristics. The Figure demonstrates a high degree of conservation among the various strains, further confirming its utility as an antigen for both vaccines and diagnostics. Fig. 21 shows an alignment comparison of amino acid sequences for ORF 287 for several strains of Neisseria. Dark shading indicates regions of homology, and gray shading indicates the conservation of amino acids with similar characteristics. The Figure demonstrates a high degree of conservation among the various strains, further confirming its utility as an antigen for both vaccines and diagnostics. Fig. 22 shows an alignment comparison of amino acid sequences for ORF 519 for several strains of Neisseria. Dark shading indicates regions of homology, and gray shading indicates the conservation of amino acids with similar characteristics. The Figure demonstrates a high degree of conservation among the various strains, further confirming its utility as an antigen for both vaccines and diagnostics. Fig. 23 shows an alignment comparison of amino acid sequences for ORF 919 for several strains of Neisseria. Dark shading indicates regions of homology, and gray shading indicates the conservation of amino acids with similar characteristics. The Figure demonstrates a high degree of conservation among the various strains, further confirming its utility as an antigen for both vaccines and diagnostics. THE INVENTION The invention provides proteins comprising the N meningitidis amino acid sequences and N gonorrhoeae amino acid sequences disclosed in the examples. It also provides proteins comprising sequences homologous (i.e., those having sequence identity) to the N. meningitidis amino acid sequences disclosed in the examples. Depending on the particular sequence, the degree of homology (sequence identity) is preferably greater than 50% (eg. 60%, 70%, 80%, 90%, 95%, 99% or more). These proteins include mutants and allelic variants of the sequences disclosed in the examples. Typically, 50% identity or more between two proteins is considered to be an indication of functional equivalence. Identity between proteins is preferably determined by the Smith- Waterman homology search algorithm as implemented in MPSRCH program (Oxford Molecular) using an affine gap search with parameters:gap penalty 12, gap extension penalty 1. The invention further provides proteins comprising fragments of the N meningitidis amino acid sequences and N gonorrhoeae amino acid sequences disclosed in the examples. The fragments should comprise at least n consecutive amino acids from the sequences and, depending on the particular sequence, n is 7 or more (eg. 8, 10, 12, 14, 16, 18, 20 or more). Preferably the fragments comprise an epitope from the sequence. The proteins of the invention can, of course, be prepared by various means (eg. recombinant expression, purification from cell culture, chemical synthesis etc.) and in various forms (eg. native, fusions etc.). They are preferably prepared in substantially pure or isolated form (ie. substantially free from other N meningitidis or N gonorrhoeae host cell proteins) According to a further aspect, the invention provides antibodies which bind to these proteins. These may be polyclonal or monoclonal and may be produced by any suitable means. According to a further aspect, the invention provides nucleic acid comprising the N meningitidis nucleotide sequences and N gonorrhoeae nucleotide sequences disclosed in the examples. According to a further aspect, the invention comprises nucleic acids having sequence identity of greater than 50% (e.g., 60%, 70%, 80%, 90%, 95%, 99% or more) to the nucleic acid sequences herein. Sequence identity is determined as above-discussed. According to a further aspect, the invention comprises nucleic acid that hybridizes to the sequences provided herein. Conditions for hybridization are set forth herein. Nucleic acid comprising fragments of these sequences are also provided. These should comprise at least n consecutive nucleotides from the N meningitidis sequences or N gonorrhoeae sequences and depending on the particular sequence, n is 10 or more (eg 12, 14, 15, 18, 20, 25, 30, 35, 40 or more). According to a further aspect, the invention provides nucleic acid encoding the proteins and protein fragments of the invention. It should also be appreciated that the invention provides nucleic acid comprising sequences complementary to those described above (eg. for antisense or probing purposes). Nucleic acid according to the invention can, of course, be prepared in many ways (eg. by chemical synthesis, in part or in whole, from genomic or cDNA libraries, from the organism itself etc.) and can take various forms (eg. single stranded, double stranded, vectors, probes etc.). In addition, the term "nucleic acid" includes DNA and RNA, and also their analogues, such as those containing modified backbones, and also protein nucleic acids (PNA) etc. According to a further aspect, the invention provides vectors comprising nucleotide sequences of the invention (eg. expression vectors) and host cells transformed with such vectors. According to a further aspect, the invention provides compositions comprising protein, antibody, and/or nucleic acid according to the invention. These compositions may be suitable as vaccines, for instance, or as diagnostic reagents or as immunogenic compositions. The invention also provides nucleic acid, protein, or antibody according to the invention for use as medicaments (eg. as vaccines) or as diagnostic reagents. It also provides the use of nucleic acid, protein, or antibody according to the invention in the manufacture of (I) a medicament for treating or preventing infection due to Neisserial bacteria (ii) a diagnostic reagent for detecting the presence of Neisserial bacteria or of antibodies raised against Neisserial bacteria or (iii) for raising antibodies. Said Neisserial bacteria may be any species or strain (such as N. gonorrhoeae) but are preferably N meningitidis, especially strain B or strain C. The invention also provides a method of treating a patient, comprising administering to the patient a therapeutically effective amount of nucleic acid, protein, and/or antibody according to the invention. According to further aspects, the invention provides various processes. A process for producing proteins of the invention is provided, comprising the step of culturing a host cell according to the invention under conditions which induce protein expression. A process for detecting polynucleotides of the invention is provided, comprising the steps of: (a) contacting a nucleic probe according to the invention with a biological sample under hybridizing conditions to form duplexes; and (b) detecting said duplexes. A process for detecting proteins of the invention is provided, comprising the steps of: (a) contacting an antibody according to the invention with a biological sample under conditions suitable for the formation of an antibody-antigen complexes; and (b) detecting said complexes. Having now generally described the inventiion, the same will be more readily understood through reference to the following examples which are provided by way of illustration, and are not intended to be limiting of the present invention, unless specified. Methodology - Summary of standard procedures and techniques. General This invention provides Neisseria meningitidis menB nucleotide sequences, amino acid sequences encoded therein. With these disclosed sequences, nucleic acid probe assays and expression cassettes and vectors can be produced. The expression vectors can be transformed into host cells to produce proteins. The purified or isolated polypeptides (which may also be chemically synthesized) can be used to produce antibodies to detect menB proteins. Also, the host cells or extracts can be utilized for biological assays to isolate agonists or antagonists. In addition, with these sequences one can search to identify open reading frames and identify amino acid sequences. The proteins may also be used in immunogenic compositions, antigenic compositions and as vaccine components. The practice of the present invention will employ, unless otherwise indicated, conventional techniques of molecular biology, microbiology, recombinant DNA, and immunology, which are within the skill of the art. Such techniques are explained fully in the literature e.g., Sambrook Molecular Cloning; A Laboratory Manual, Second Edition (1989); DNA Cloning, Volumes I and ii (D.N Glover ed. 1985); Oligonucleotide Synthesis (M.J. Gait ed, 1984); Nucleic Acid Hybridization (B.D. Hames & S.J. Higgins eds. 1984); Transcription and Translation (B.D. Hames & S.J. Higgins eds. 1984); Animal Cell Culture (R.I. Freshney ed. 1986); Immobilized Cells and Enzymes (IRL Press, 1986); B. Perbal, A Practical Guide to Molecular Cloning (1984); the Methods in Enzymology series (Academic Press, Inc.), especially volumes 154 & 155; Gene Transfer Vectors for Mammalian Cells (J.H. Miller and M.P. Calos eds. 1987, Cold Spring Harbor Laboratory); Mayer and Walker, eds. (1987), Immunochemical Methods in Cell and Molecular Biology (Academic Press, London); Scopes, (1987) Protein Purification: Principles and Practice, Second Edition (Springer- Verlag, N.Y.), and Handbook of Experimental Immunology, Volumes I-IV (D.M. Weir and C.C. Blackwell eds 1986). Standard abbreviations for nucleotides and amino acids are used in this specification. All publications, patents, and patent applications cited herein are incorporated in full by reference. Expression systems The Neisseria menB nucleotide sequences can be expressed in a variety of different expression systems; for example those used with mammalian cells, plant cells, baculoviruses, bacteria, and yeast. i. Mammalian Systems Mammalian expression systems are known in the art. A mammalian promoter is any DNA sequence capable of binding mammalian RNA polymerase and initiating the downstream (3') transcription of a coding sequence (e.g., structural gene) into mRNA. A promoter will have a transcription initiating region, which is usually placed proximal to the 5' end of the coding sequence, and a TATA box, usually located 25-30 base pairs (bp) upstream of the transcription initiation site. The TATA box is thought to direct RNA polymerase II to begin RNA synthesis at the correct site. A mammalian promoter will also contain an upstream promoter element, usually located within 100 to 200 bp upstream of the TATA box. An upstream promoter element determines the rate at which transcription is initiated and can act in either orientation (Sambrook et al. (1989) "Expression of Cloned Genes in Mammalian Cells." In Molecular Cloning: A Laboratory Manual, 2nd ed.). Mammalian viral genes are often highly expressed and have a broad host range; therefore sequences encoding mammalian viral genes provide particularly useful promoter sequences. Examples include the SV40 early promoter, mouse mammary tumor virus LTR promoter, adenovirus major late promoter (Ad MLP), and herpes simplex virus promoter. In addition, sequences derived from non-viral genes, such as the murine metallothionein gene, also provide useful promoter sequences. Expression may be either constitutive or regulated (inducible). Depending on the promoter selected, many promotes may be inducible using known substrates, such as the use of the mouse mammary tumor virus (MMTV) promoter with the glucocorticoid responsive element (GRE) that is induced by glucocorticoid in hormone-responsive transformed cells (see for example, U.S. Patent 5,783,681). The presence of an enhancer element (enhancer), combined with the promoter elements described above, will usually increase expression levels. An enhancer is a regulatory DNA sequence that can stimulate transcription up to 1000-fold when linked to homologous or heterologous promoters, with synthesis beginning at the normal RNA start site. Enhancers are also active when they are placed upstream or downstream from the transcription initiation site, in either normal or flipped orientation, or at a distance of more than 1000 nucleotides from the promoter (Maniatis et al. (1987) Science 236:1231; Alberts et al. (1989) Molecular Biology of the Cell, 2nd ed.). Enhancer elements derived from viruses may be particularly useful, because they usually have a broader host range. Examples include the SV40 early gene enhancer (Dijkema et al (1985) EMBO J. 4:161) and the enhancer/promoters derived from the long terminal repeat (LTR) of the Rous Sarcoma Virus (Gorman et al. (1982b) Proc. Natl. Acad. Sci. 79:6111) and from human cytomegalovirus (Boshart et al. (1985) Cell 41:521). Additionally, some enhancers are regulatable and become active only in the presence of an inducer, such as a hormone or metal ion (Sassone-Corsi and Borelli (1986) Trends Genet. 2:215; Maniatis et al. (1987) Science 236:1237). A DNA molecule may be expressed intracellularly in mammalian cells. A promoter sequence may be directly linked with the DNA molecule, in which case the first amino acid at the N-terminus of the recombinant protein will always be a methionine, which is encoded by the ATG start codon. If desired, the N-terminus may be cleaved from the protein by in vitro incubation with cyanogen bromide. Alternatively, foreign proteins can also be secreted from the cell into the growth media by creating chimeric DNA molecules that encode a fusion protein comprised of a leader sequence fragment that provides for secretion of the foreign protein in mammalian cells. Preferably, there are processing sites encoded between the leader fragment and the foreign gene that can be cleaved either in vivo or in vitro. The leader sequence fragment usually encodes a signal peptide comprised of hydrophobic amino acids which direct the secretion of the protein from the cell. The adenovirus tripartite leader is an example of a leader sequence that provides for secretion of a foreign protein in mammalian cells. Usually, transcription termination and polyadenylation sequences recognized by mammalian cells are regulatory regions located 3' to the translation stop codon and thus, together with the promoter elements, flank the coding sequence. The 3' terminus of the mature mRNA is formed by site-specific post-transcriptional cleavage and polyadenylation (Birnstiel et al. (1985) Cell 41:349; Proudfoot and Whitelaw (1988) "Termination and 3' end processing of eukaryotic RNA. In Transcription and splicing (ed. B.D. Hames and D.M. Glover); Proudfoot (1989) Trends Biochem. Sci. 14:105). These sequences direct the transcription of an mRNA which can be translated into the polypeptide encoded by the DNA. Examples of transcription terminator/polyadenylation signals include those derived from SV40 (Sambrook et al (1989) "Expression of cloned genes in cultured mammalian cells." In Molecular Cloning: A Laboratory Manual). Usually, the above described components, comprising a promoter, polyadenylation signal, and transcription termination sequence are put together into expression constructs. Enhancers, introns with functional splice donor and acceptor sites, and leader sequences may also be included in an expression construct, if desired. Expression constructs are often maintained in a replicon, such as an extrachromosomal element (e.g., plasmids) capable of stable maintenance in a host, such as mammalian cells or bacteria. Mammalian replication systems include those derived from animal viruses, which require trans-acting factors to replicate. For example, plasmids containing the replication systems of papovaviruses, such as SV40 (Gluzman (1981) Cell 23:115) or polyomavirus, replicate to extremely high copy number in the presence of the appropriate viral T antigen. Additional examples of mammalian replicons include those derived from bovine papillomavirus and Epstein-Barr virus. Additionally, the replicon may have two replication systems, thus allowing it to be maintained, for example, in mammalian cells for expression and in a prokaryotic host for cloning and amplification. Examples of such mammalian-bacteria shuttle vectors include pMT2 (Kaufman et al. (1989) Mol. Cell. Biol. 9:946) and pHEBO (Shimizu et al. (1986) Mol. Cell. Biol. 6:1014). The transformation procedure used depends upon the host to be transformed. Methods for introduction of heterologous polynucleotides into mammalian cells are known in the art and include dextran-mediated transfection, calcium phosphate precipitation, polybrene mediated transfection, protoplast fusion, electroporation, encapsulation of the polynucleotide(s) in liposomes, and direct microinjection of the DNA into nuclei. Mammalian cell lines available as hosts for expression are known in the art and include many immortalized cell lines available from the American Type Culture Collection (ATCC), including but not limited to, Chinese hamster ovary (CHO) cells, HeLa cells, baby hamster kidney (BHK) cells, monkey kidney cells (COS), human hepatocellular carcinoma cells (e.g., Hep G2), and a number of other cell lines. ii. Plant Cellular Expression Systems There are many plant cell culture and whole plant genetic expression systems known in the art. Exemplary plant cellular genetic expression systems include those described in patents, such as: U.S. 5,693,506; US 5,659,122; and US 5,608,143. Additional examples of genetic expression in plant cell culture has been described by Zenk, Phytochemistry 30:3861- 3863 (1991). Descriptions of plant protein signal peptides may be found in addition to the references described above in Vaulcombe et al., Mol. Gen. Genet. 209:33-40 (1987); Chandler et al, Plant Molecular Biology 3:407-418 (1984); Rogers, J. Biol Chem. 260:3731-3738 (1985); Rothstein et al., Gene 55:353-356 (1987); Whittier et al, Nucleic Acids Research 15:2515-2535 (1987); Wirsel et al., Molecular Microbiology 3:3-14 (1989); Yu et al, Gene 122:247-253 (1992). A description of the regulation of plant gene expression by the phytohormone, gibberellic acid and secreted enzymes induced by gibberellic acid can be found in R.L. Jones and J. MacMillin, Gibberellins: in: Advanced Plant Physiology,. Malcolm B. Wilkins, ed., 1984 Pitman Publishing Limited, London, pp. 21-52. References that describe other metabolically-regulated genes: Sheen, Plant Cell, 2:1027-1038(1990); Maas et al., EMBOJ. 9:3447-3452 (1990); Benkel and Hickey, Proc. Natl. Acad. Sci. 84:1337-1339 (1987) Typically, using techniques known in the art, a desired polynucleotide sequence is inserted into an expression cassette comprising genetic regulatory elements designed for operation in plants. The expression cassette is inserted into a desired expression vector with companion sequences upstream and downstream from the expression cassette suitable for expression in a plant host. The companion sequences will be of plasmid or viral origin and provide necessary characteristics to the vector to permit the vectors to move DNA from an original cloning host, such as bacteria, to the desired plant host. The basic bacterial/plant vector construct will preferably provide a broad host range prokaryote replication origin; a prokaryote selectable marker; and, for Agrobacterium transformations, T DNA sequences for Agrobacterium-mediated transfer to plant chromosomes. Where the heterologous gene is not readily amenable to detection, the construct will preferably also have a selectable marker gene suitable for determining if a plant cell has been transformed. A general review of suitable markers, for example for the members of the grass family, is found in Wilmink and Dons, 1993, Plant Mol. Biol. Reptr, 11(2):165-185. Sequences suitable for permitting integration of the heterologous sequence into the plant genome are also recommended. These might include transposon sequences and the like for homologous recombination as well as Ti sequences which permit random insertion of a heterologous expression cassette into a plant genome. Suitable prokaryote selectable markers include resistance toward antibiotics such as ampicillin or tetracycline. Other DNA sequences encoding additional functions may also be present in the vector, as is known in the art. The nucleic acid molecules of the subject invention may be included into an expression cassette for expression of the protein(s) of interest. Usually, there will be only one expression cassette, although two or more are feasible. The recombinant expression cassette will contain in addition to the heterologous protein encoding sequence the following elements, a promoter region, plant 5' untranslated sequences, initiation codon depending upon whether or not the structural gene comes equipped with one, and a transcription and translation termination sequence. Unique restriction enzyme sites at the 5' and 3' ends of the cassette allow for easy insertion into a pre-existing vector. A heterologous coding sequence may be for any protein relating to the present invention. The sequence encoding the protein of interest will encode a signal peptide which allows processing and translocation of the protein, as appropriate, and will usually lack any sequence which might result in the binding of the desired protein of the invention to a membrane. Since, for the most part, the transcriptional initiation region will be for a gene which is expressed and translocated during germination, by employing the signal peptide which provides for translocation, one may also provide for translocation of the protein of interest. In this way, the protein(s) of interest will be translocated from the cells in which they are expressed and may be efficiently harvested. Typically secretion in seeds are across the aleurone or scutellar epithelium layer into the endosperm of the seed. While it is not required that the protein be secreted from the cells in which the protein is produced, this facilitates the isolation and purification of the recombinant protein. Since the ultimate expression of the desired gene product will be in a eucaryotic cell it is desirable to determine whether any portion of the cloned gene contains sequences which will be processed out as introns by the host's splicosome machinery. If so, site-directed mutagenesis of the "intron" region may be conducted to prevent losing a portion of the genetic message as a false intron code, Reed and Maniatis, Cell 41:95-105, 1985. The vector can be microinjected directly into plant cells by use of micropipettes to mechanically transfer the recombinant DNA. Crossway, Mol. Gen. Genet, 202:179-185, 1985. The genetic material may also be transferred into the plant cell by using polyethylene glycol, Krens, et al., Nature, 296, 72-74, 1982. Another method of introduction of nucleic acid segments is high velocity ballistic penetration by small particles with the nucleic acid either within the matrix of small beads or particles, or on the surface, Klein, et al., Nature, 327, 70-73, 1987 and Knudsen and Muller, 1991, Planta, 185:330-336 teaching particle bombardment of barley endosperm to create transgenic barley. Yet another method of introduction would be fusion of protoplasts with other entities, either minicells, cells, lysosomes or other fusible lipid-surfaced bodies, Fraley, et al., Proc. Natl. Acad. Sci. USA, 79, 1859-1863, 1982. The vector may also be introduced into the plant cells by electroporation. (Fromm et al., Proc. Natl Acad. Sci. USA 82:5824, 1985). In this technique, plant protoplasts are electroporated in the presence of plasmids containing the gene construct. Electrical impulses of high field strength reversibly permeabilize biomembranes allowing the introduction of the plasmids. Electroporated plant protoplasts reform the cell wall, divide, and form plant callus. All plants from which protoplasts can be isolated and cultured to give whole regenerated plants can be transformed by the present invention so that whole plants are recovered which contain the transferred gene. It is known that practically all plants can be regenerated from cultured cells or tissues, including but not limited to all major species of sugarcane, sugar beet, cotton, fruit and other trees, legumes and vegetables. Some suitable plants include, for example, species from the genera Fragaria, Lotus, Medicago, Onobrychis, Trifolium, Trigonella, Vigna, Citrus, Linum, Geranium, Manihot, Daucus, Arabidopsis, Brassica, Raphanus, Sinapis, Atropa, Capsicum, Datura, Hyoscyamus, Lycopersion, Nicotiana, Solanum, Petunia, Digitalis, Majorana, Cichorium, Helianthus, Lactuca, Bromus, Asparagus, Antirrhinum, Hererocallis, Nemesia, Pelargonium, Panicum, Pennisetum, Ranunculus, Senecio, Salpiglossis, Cucumis, Browaalia, Glycine, Lolium, Zea, Triticum, Sorghum, and Datura. Means for regeneration vary from species to species of plants, but generally a suspension of transformed protoplasts containing copies of the heterologous gene is first provided. Callus tissue is formed and shoots may be induced from callus and subsequently rooted. Alternatively, embryo formation can be induced from the protoplast suspension. These embryos germinate as natural embryos to form plants. The culture media will generally contain various amino acids and hormones, such as auxin and cytokinins. It is also advantageous to add glutamic acid and proline to the medium, especially for such species as corn and alfalfa. Shoots and roots normally develop simultaneously. Efficient regeneration will depend on the medium, on the genotype, and on the history of the culture. If these three variables are controlled, then regeneration is fully reproducible and repeatable. In some plant cell culture systems, the desired protein of the invention may be excreted or alternatively, the protein may be extracted from the whole plant. Where the desired protein of the invention is secreted into the medium, it may be collected. Alternatively, the embryos and embryoless-half seeds or other plant tissue may be mechanically disrupted to release any secreted protein between cells and tissues. The mixture may be suspended in a buffer solution to retrieve soluble proteins. Conventional protein isolation and purification methods will be then used to purify the recombinant protein. Parameters of time, temperature pH, oxygen, and volumes will be adjusted through routine methods to optimize expression and recovery of heterologous protein. iii. Baculovirus Systems The polynucleotide encoding the protein can also be inserted into a suitable insect expression vector, and is operably linked to the control elements within that vector. Vector construction employs techniques which are known in the art. Generally, the components of the expression system include a transfer vector, usually a bacterial plasmid, which contains both a fragment of the baculovirus genome, and a convenient restriction site for insertion of the heterologous gene or genes to be expressed; a wild type baculovirus with a sequence homologous to the baculovirus-specific fragment in the transfer vector (this allows for the homologous recombination of the heterologous gene in to the baculovirus genome); and appropriate insect host cells and growth media. After inserting the DNA sequence encoding the protein into the transfer vector, the vector and the wild type viral genome are transfected into an insect host cell where the vector and viral genome are allowed to recombine. The packaged recombinant virus is expressed and recombinant plaques are identified and purified. Materials and methods for baculovirus/insect cell expression systems are commercially available in kit form from, inter alia, Invitrogen, San Diego CA ("MaxBac" kit). These techniques are generally known to those skilled in the art and fully described in Summers and Smith, Texas Agricultural Experiment Station Bulletin No. 1555 (1987) (hereinafter "Summers and Smith"). Prior to inserting the DNA sequence encoding the protein into the baculovirus genome, the above described components, comprising a promoter, leader (if desired), coding sequence of interest, and transcription termination sequence, are usually assembled into an intermediate transplacement construct (transfer vector). This construct may contain a single gene and operably linked regulatory elements; multiple genes, each with its owned set of operably linked regulatory elements; or multiple genes, regulated by the same set of regulatory elements. Intermediate transplacement constructs are often maintained in a replicon, such as an extrachromosomal element (e.g., plasmids) capable of stable maintenance in a host, such as a bacterium. The replicon will have a replication system, thus allowing it to be maintained in a suitable host for cloning and amplification. Currently, the most commonly used transfer vector for introducing foreign genes into AcNPV is pAc373. Many other vectors, known to those of skill in the art, have also been designed. These include, for example, pVL985 (which alters the polyhedrin start codon from ATG to ATT, and which introduces a BamHI cloning site 32 basepairs downstream from the ATT; see Luckow and Summers, Virology (1989) 7 :31. The plasmid usually also contains the polyhedrin polyadenylation signal (Miller et al. (1988) Ann. Rev. Microbiol, 42:111) and a prokaryotic ampicillin-resistance (amp) gene and origin of replication for selection and propagation in E. coli. Baculovirus transfer vectors usually contain a baculovirus promoter. A baculovirus promoter is any DNA sequence capable of binding a baculovirus RNA polymerase and initiating the downstream (5' to 3') transcription of a coding sequence (e.g., structural gene) into mRNA. A promoter will have a transcription initiation region which is usually placed proximal to the 5' end of the coding sequence. This transcription initiation region usually includes an RNA polymerase binding site and a transcription initiation site. A baculovirus transfer vector may also have a second domain called an enhancer, which, if present, is usually distal to the structural gene. Expression may be either regulated or constitutive. Structural genes, abundantly transcribed at late times in a viral infection cycle, provide particularly useful promoter sequences. Examples include sequences derived from the gene encoding the viral polyhedron protein, Friesen et al., (1986) "The Regulation of Baculovirus Gene Expression," in: The Molecular Biology of Baculoviruses (ed. Walter Doerfier); EPO Publ. Nos. 127 839 and 155 476; and the gene encoding the plO protein, Vlak et al, (1988), J. Gen. Virol. 69:165. DNA encoding suitable signal sequences can be derived from genes for secreted insect or baculovirus proteins, such as the baculovirus polyhedrin gene (Carbonell et al. (1988) Gene, 73:409). Alternatively, since the signals for mammalian cell posttranslational modifications (such as signal peptide cleavage, proteolytic cleavage, and phosphorylation) appear to be recognized by insect cells, and the signals required for secretion and nuclear accumulation also appear to be conserved between the invertebrate cells and vertebrate cells, leaders of non-insect origin, such as those derived from genes encoding human (alpha) α- interferon, Maeda et al., (1985), Nature 315:592; human gastrin-releasing peptide, Lebacq- Verheyden et al., (1988), Molec. Cell. Biol. 5:3129; human IL-2, Smith et al, (1985) Proc. Nat'lAcad. Sci. USA, 52:8404; mouse IL-3, (Miyajima et al., (1987) Gene 58:213; and human glucocerebrosidase, Martin et al. (1988) DNA, 7:99, can also be used to provide for secretion in insects. A recombinant polypeptide or polyprotein may be expressed intracellularly or, if it is expressed with the proper regulatory sequences, it can be secreted. Good intracellular expression of nonfused foreign proteins usually requires heterologous genes that ideally have a short leader sequence containing suitable translation initiation signals preceding an ATG start signal. If desired, methionine at the N-terminus may be cleaved from the mature protein by in vitro incubation with cyanogen bromide. Alternatively, recombinant polyproteins or proteins which are not naturally secreted can be secreted from the insect cell by creating chimeric DNA molecules that encode a fusion protein comprised of a leader sequence fragment that provides for secretion of the foreign protein in insects. The leader sequence fragment usually encodes a signal peptide comprised of hydrophobic amino acids which direct the translocation of the protein into the endoplasmic reticulum. After insertion of the DNA sequence and/or the gene encoding the expression product precursor of the protein, an insect cell host is co-transformed with the heterologous DNA of the transfer vector and the genomic DNA of wild type baculovirus - usually by co- transfection. The promoter and transcription termination sequence of the construct will usually comprise a 2-5kb section of the baculovirus genome. Methods for introducing heterologous DNA into the desired site in the baculovirus virus are known in the art. (See Summers and Smith supra; Ju et al. (1987); Smith et al., Mol Cell. Biol. (1983) 5:2156; and Luckow and Summers (1989)). For example, the insertion can be into a gene such as the polyhedrin gene, by homologous double crossover recombination; insertion can also be into a restriction enzyme site engineered into the desired baculovirus gene. Miller et al., (1989), Bioessays 4:91. The DNA sequence, when cloned in place of the polyhedrin gene in the expression vector, is flanked both 5' and 3' by polyhedrin-specific sequences and is positioned downstream of the polyhedrin promoter. The newly formed baculovirus expression vector is subsequently packaged into an infectious recombinant baculovirus. Homologous recombination occurs at low frequency (between about 1% and about 5%); thus, the majority of the virus produced after cotransfection is still wild-type virus. Therefore, a method is necessary to identify recombinant viruses. An advantage of the expression system is a visual screen allowing recombinant viruses to be distinguished. The polyhedrin protein, which is produced by the native virus, is produced at very high levels in the nuclei of infected cells at late times after viral infection. Accumulated polyhedrin protein forms occlusion bodies that also contain embedded particles. These occlusion bodies, up to 15 μm in size, are highly refractile, giving them a bright shiny appearance that is readily visualized under the light microscope. Cells infected with recombinant viruses lack occlusion bodies. To distinguish recombinant virus from wild-type virus, the transfection supernatant is plaqued onto a monolayer of insect cells by techniques known to those skilled in the art. Namely, the plaques are screened under the light microscope for the presence (indicative of wild-type virus) or absence (indicative of recombinant virus) of occlusion bodies. Current Protocols in Microbiology Vol. 2 (Ausubel et al. eds) at 16.8 (Supp. 10, 1990); Summers and Smith, supra; Miller et al. (1989). Recombinant baculovirus expression vectors have been developed for infection into several insect cells. For example, recombinant baculoviruses have been developed for, inter alia: Aedes aegypti , Autographa californica, Bombyx mori, Drosophila melanogaster, Spodoptera frugiperda, and Trichoplusia ni (PCT Pub. No. WO 89/046699; Carbonell et al., (1985) J. Virol. 56:153; Wright (1986) Nature 321:118; Smith et al., (1983) Mol. Cell. Biol. 3:2156; and see generally, Fraser, et al. (1989) In Vitro Cell. Dev. Biol. 25:225). Cells and cell culture media are commercially available for both direct and fusion expression of heterologous polypeptides in a baculovirus/expression system; cell culture technology is generally known to those skilled in the art. See, e.g., Summers and Smith supra. The modified insect cells may then be grown in an appropriate nutrient medium, which allows for stable maintenance of the plasmid(s) present in the modified insect host. Where the expression product gene is under inducible control, the host may be grown to high density, and expression induced. Alternatively, where expression is constitutive, the product will be continuously expressed into the medium and the nutrient medium must be continuously circulated, while removing the product of interest and augmenting depleted nutrients. The product may be purified by such techniques as chromatography, e.g., HPLC, affinity chromatography, ion exchange chromatography, etc.; electrophoresis; density gradient centrifugation; solvent extraction, or the like. As appropriate, the product may be further purified, as required, so as to remove substantially any insect proteins which are also secreted in the medium or result from lysis of insect cells, so as to provide a product which is at least substantially free of host debris, e.g., proteins, lipids and polysaccharides. In order to obtain protein expression, recombinant host cells derived from the transformants are incubated under conditions which allow expression of the recombinant protein encoding sequence. These conditions will vary, dependent upon the host cell selected. However, the conditions are readily ascertainable to those of ordinary skill in the art, based upon what is known in the art. iv. Bacterial Systems Bacterial expression techniques are known in the art. A bacterial promoter is any DNA sequence capable of binding bacterial RNA polymerase and initiating the downstream (3') transcription of a coding sequence (e.g. structural gene) into mRNA. A promoter will have a transcription initiation region which is usually placed proximal to the 5' end of the coding sequence. This transcription initiation region usually includes an RNA polymerase binding site and a transcription initiation site. A bacterial promoter may also have a second domain called an operator, that may overlap an adjacent RNA polymerase binding site at which RNA synthesis begins. The operator permits negative regulated (inducible) transcription, as a gene repressor protein may bind the operator and thereby inhibit transcription of a specific gene. Constitutive expression may occur in the absence of negative regulatory elements, such as the operator. In addition, positive regulation may be achieved by a gene activator protein binding sequence, which, if present is usually proximal (5') to the RNA polymerase binding sequence. An example of a gene activator protein is the catabolite activator protein (CAP), which helps initiate transcription of the lac operon in Escherichia coli (E. coli) (Raibaud et al. (1984) Annu. Rev. Genet. 18:113). Regulated expression may therefore be either positive or negative, thereby either enhancing or reducing transcription. Sequences encoding metabolic pathway enzymes provide particularly useful promoter sequences. Examples include promoter sequences derived from sugar metabolizing enzymes, such as galactose, lactose (lac) (Chang et al. (1977) Nature 198: 056), and maltose. Additional examples include promoter sequences derived from biosynthetic enzymes such as tryptophan (trp) (Goeddel et al. (1980) Nuc. Acids Res. 8:4051; Yelverton et al. (1981) Nucl. Acids Res. 9:131; U.S. Patent 4,738,921; EPO Publ. Nos. 036 776 and 121 775). The beta- lactamase (bid) promoter system (Weissmann (1981) "The cloning of interferon and other mistakes." In Interferon 3 (ed. I. Gresser)), bacteriophage lambda PL (Shimatake et al. (1981) Nature 292: 2%) and T5 (U.S. Patent 4,689,406) promoter systems also provide useful promoter sequences. In addition, synthetic promoters which do not occur in nature also function as bacterial promoters. For example, transcription activation sequences of one bacterial or bacteriophage promoter may be joined with the operon sequences of another bacterial or bacteriophage promoter, creating a synthetic hybrid promoter (U.S. Patent 4,551,433). For example, the tac promoter is a hybrid trp-lac promoter comprised of both trp promoter and lac operon sequences that is regulated by the lac repressor (Amann et al. (1983) Gene 25:161; de Boer et al. (1983) Proc. Natl. Acad. Sci. 80:2 ). Furthermore, a bacterial promoter can include naturally occurring promoters of non-bacterial origin that have the ability to bind bacterial RNA polymerase and initiate transcription. A naturally occurring promoter of non-bacterial origin can also be coupled with a compatible RNA polymerase to produce high levels of expression of some genes in prokaryotes. The bacteriophage T7 RNA polymerase/promoter system is an example of a coupled promoter system (Studier et al. (1986) J. Mol. Biol. 189:113; Tabor et al. (1985) Proc Natl. Acad. Sci. 82:1014). In addition, a hybrid promoter can also be comprised of a bacteriophage promoter and an E. coli operator region (EPO Publ. No. 267 851). In addition to a functioning promoter sequence, an efficient ribosome binding site is also useful for the expression of foreign genes in prokaryotes. In E. coli, the ribosome binding site is called the Shine-Dalgarno (SD) sequence and includes an initiation codon (ATG) and a sequence 3-9 nucleotides in length located 3-11 nucleotides upstream of the initiation codon (Shine et al. (1975) Nature 254:34). The SD sequence is thought to promote binding of mRNA to the ribosome by the pairing of bases between the SD sequence and the 3' end of E. coli 16S rRNA (Steitz et al. (1979) "Genetic signals and nucleotide sequences in messenger RNA." In Biological Regulation and Development: Gene Expression (ed. R.F. Goldberger)). To express eukaryotic genes and prokaryotic genes with weak ribosome- binding site, it is often necessary to optimize the distance between the SD sequence and the ATG of the eukaryotic gene (Sambrook et al. (1989) "Expression of cloned genes in Escherichia coli." la Molecular Cloning: A Laboratory Manual). A DNA molecule may be expressed intracellularly. A promoter sequence may be directly linked with the DNA molecule, in which case the first amino acid at the N-terminus will always be a methionine, which is encoded by the ATG start codon. If desired, methionine at the N-terminus may be cleaved from the protein by in vitro incubation with cyanogen bromide or by either in vivo or in vitro incubation with a bacterial methionine N-terminal peptidase (EPO Publ. No. 219 237). Fusion proteins provide an alternative to direct expression. Usually, a DNA sequence encoding the N-terminal portion of an endogenous bacterial protein, or other stable protein, is fused to the 5' end of heterologous coding sequences. Upon expression, this construct will provide a fusion of the two amino acid sequences. For example, the bacteriophage lambda cell gene can be linked at the 5' terminus of a foreign gene and expressed in bacteria. The resulting fusion protein preferably retains a site for a processing enzyme (factor Xa) to cleave the bacteriophage protein from the foreign gene (Nagai et al (1984) Nature 309:810). Fusion proteins can also be made with sequences from the lacϊ (Jia et al. (1987) Gene 60:191), trpE (Allen et al. (1987) J. Biotechnol. 5:93; Makoff et al. (1989) J. Gen. Microbiol 135:11), and Chey (EPO Publ. No. 324 647) genes. The DNA sequence at the junction of the two amino acid sequences may or may not encode a cleavable site. Another example is a ubiquitin fusion protein. Such a fusion protein is made with the ubiquitin region that preferably retains a site for a processing enzyme (e.g. ubiquitin specific processing-protease) to cleave the ubiquitin from the foreign protein. Through this method, native foreign protein can be isolated (Miller et al. (1989) Bio/Technology 7:698). Alternatively, foreign proteins can also be secreted from the cell by creating chimeric DNA molecules that encode a fusion protein comprised of a signal peptide sequence fragment that provides for secretion of the foreign protein in bacteria (U.S. Patent 4,336,336). The signal sequence fragment usually encodes a signal peptide comprised of hydrophobic amino acids which direct the secretion of the protein from the cell. The protein is either secreted into the growth media (gram-positive bacteria) or into the periplasmic space, located between the inner and outer membrane of the cell (gram-negative bacteria). Preferably there are processing sites, which can be cleaved either in vivo or in vitro encoded between the signal peptide fragment and the foreign gene. DNA encoding suitable signal sequences can be derived from genes for secreted bacterial proteins, such as the E. coli outer membrane protein gene (ompA) (Masui et al. (1983), in: Experimental Manipulation of Gene Expression; Ghrayeb et al. (1984) EMBO J. 3:2431) and the E. coli alkaline phosphatase signal sequence (phoA) (Oka et al. (1985) Proc. Natl. Acad. Sci. 82:1212). As an additional example, the signal sequence of the alpha-amylase gene from various Bacillus strains can be used to secrete heterologous proteins from B. subtilis (Palva et al. (1982) Proc. Natl. Acad. Sci. USA 79:5582; ΕPO Publ. No. 244 042). Usually, transcription termination sequences recognized by bacteria are regulatory regions located 3' to the translation stop codon, and thus together with the promoter flank the coding sequence. These sequences direct the transcription of an mRNA which can be translated into the polypeptide encoded by the DNA. Transcription termination sequences frequently include DNA sequences of about 50 nucleotides capable of forming stem loop structures that aid in terminating transcription. Examples include transcription termination sequences derived from genes with strong promoters, such as the trp gene in E. coli as well as other biosynthetic genes. Usually, the above described components, comprising a promoter, signal sequence (if desired), coding sequence of interest, and transcription termination sequence, are put together into expression constructs. Expression constructs are often maintained in a replicon, such as an extrachromosomal element (e.g., plasmids) capable of stable maintenance in a host, such as bacteria. The replicon will have a replication system, thus allowing it to be maintained in a prokaryotic host either for expression or for cloning and amplification. In addition, a replicon may be either a high or low copy number plasmid. A high copy number plasmid will generally have a copy number ranging from about 5 to about 200, and usually about 10 to about 150. A host containing a high copy number plasmid will preferably contain at least about 10, and more preferably at least about 20 plasmids. Either a high or low copy number vector may be selected, depending upon the effect of the vector and the foreign protein on the host. Alternatively, the expression constructs can be integrated into the bacterial genome with an integrating vector. Integrating vectors usually contain at least one sequence homologous to the bacterial chromosome that allows the vector to integrate. Integrations appear to result from recombinations between homologous DNA in the vector and the bacterial chromosome. For example, integrating vectors constructed with DNA from various Bacillus strains integrate into the Bacillus chromosome (EPO Publ. No. 127 328). Integrating vectors may also be comprised of bacteriophage or transposon sequences. Usually, extrachromosomal and integrating expression constructs may contain selectable markers to allow for the selection of bacterial strains that have been transformed. Selectable markers can be expressed in the bacterial host and may include genes which render bacteria resistant to drugs such as ampicillin, chloramphenicol, erythromycin, kanamycin (neomycin), and tetracycline (Davies et al. (1918) Annu. Rev. Microbiol 32:469). Selectable markers may also include biosynthetic genes, such as those in the histidine, tryptophan, and leucine biosynthetic pathways. Alternatively, some of the above described components can be put together in transformation vectors. Transformation vectors are usually comprised of a selectable market that is either maintained in a replicon or developed into an integrating vector, as described above. Expression and transformation vectors, either extra-chromosomal replicons or integrating vectors, have been developed for transformation into many bacteria. For example, expression vectors have been developed for, inter alia, the following bacteria: Bacillus subtilis (Palva et al. (1982) Proc. Natl. Acad. Sci. USA 79:5582; EPO Publ. Nos. 036 259 and 063 953; PCT Publ. No. WO 84/04541), Escherichia coli (Shimatake et al. (1981) Nature 292:128; Amann et al. (1985) Gene 40:183; Studier et al. (1986) J. Mol Biol. 189:113; EPO Publ. Nos. 036 776, 136 829 and 136 907), Streptococcus cremoris (Powell et al. (1988) Appl Environ. Microbiol. 54:655); Streptococcus lividans (Powell et al. (1988) Appl. Environ. Microbiol 54:655), Streptomyces lividans (U.S. Patent 4,745,056). Methods of introducing exogenous DNA into bacterial hosts are well-known in the art, and usually include either the transformation of bacteria treated with CaCl or other agents, such as divalent cations and DMSO. DNA can also be introduced into bacterial cells by electroporation. Transformation procedures usually vary with the bacterial species to be transformed. (See e.g., use of Bacillus: Masson et al. (1989) F EMS Microbiol. Lett. 60:213; Palva et al. (1982) Proc. Natl. Acad. Sci. USA 79:5582; EPO Publ. Nos. 036 259 and 063 953; PCT Publ. No. WO 84/04541; use of Campylobacter: Miller et al. (1988) Proc. Natl. Acad. Sci. 85:856; and Wang et al. (1990) J. Bacteriol. 172:949; use of Escherichia coli: Cohen et al. ( 913) Proc. Natl. Acad. Sci. 69:2110; Dower et al. (1988) Nucleic Acids Res. 16:6121; Kushner (1978) "An improved method for transformation of Escherichia coli with ColEl- derived plasmids. In Genetic Engineering: Proceedings of the International Symposium on Genetic Engineering (eds. H.W. Boyer and S. Nicosia); Mandel et al. (1970) J. Mol. Biol. 53:159; Taketo (1988) Biochim. Biophys. Acta 949:318; use of Lactobacillus: Chassy et al. (1987) FEMS Microbiol. Lett. 44:113; use of Pseudomonas: Fiedler et al. (1988) Anal Biochem 170:38; use of Staphylococcus: Augustin et al. (1990) FEMS Microbiol. Lett. 66:203; use of Streptococcus: Barany et al. (1980) J. Bacteriol. 144:698; Harlander (1987) "Transformation of Streptococcus lactis by electroporation, in: Streptococcal Genetics (ed. J. Ferretti and R. Curtiss III); Perry et al. (1981) Infect. Immun. 32:1295; Powell et al. (1988) Appl. Environ. Microbiol. 54:655; Somkuti et al. (1987) Proc. 4th Evr. Cong. Biotechnology 1:412. v. Yeast Expression Yeast expression systems are also known to one of ordinary skill in the art. A yeast promoter is any DNA sequence capable of binding yeast RNA polymerase and initiating the downstream (3') transcription of a coding sequence (e.g. structural gene) into mRNA. A promoter will have a transcription initiation region which is usually placed proximal to the 5' end of the coding sequence. This transcription initiation region usually includes an RNA polymerase binding site (the "TATA Box") and a transcription initiation site. A yeast promoter may also have a second domain called an upstream activator sequence (UAS), which, if present, is usually distal to the structural gene. The UAS permits regulated (inducible) expression. Constitutive expression occurs in the absence of a UAS. Regulated expression may be either positive or negative, thereby either enhancing or reducing transcription. Yeast is a fermenting organism with an active metabolic pathway, therefore sequences encoding enzymes in the metabolic pathway provide particularly useful promoter sequences. Examples include alcohol dehydrogenase (ADH) (EPO Publ. No. 284 044), enolase, glucokinase, glucose-6-phosphate isomerase, glyceraldehyde-3-phosphate-dehydrogenase (GAP or GAPDH), hexokinase, phosphofructokinase, 3-phosphoglycerate mutase, and pyruvate kinase (PyK) (EPO Publ. No. 329 203). The yeast PH05 gene, encoding acid phosphatase, also provides useful promoter sequences (Myanohara et al. (1983) Proc. Natl Acad. Sci. USA 80:1). In addition, synthetic promoters which do not occur in nature also function as yeast promoters. For example, UAS sequences of one yeast promoter may be joined with the transcription activation region of another yeast promoter, creating a synthetic hybrid promoter. Examples of such hybrid promoters include the ADH regulatory sequence linked to the GAP transcription activation region (U.S. Patent Nos. 4,876,197 and 4,880,734). Other examples of hybrid promoters include promoters which consist of the regulatory sequences of either the ADH2, GAL4, GAL10, OR PH05 genes, combined with the transcriptional activation region of a glycolytic enzyme gene such as GAP or PyK (EPO Publ. No. 164 556). Furthermore, a yeast promoter can include naturally occurring promoters of non-yeast origin that have the ability to bind yeast RNA polymerase and initiate transcription. Examples of such promoters include, mter alia, (Cohen et al. (1980) Proc. Natl. Acad. Sci. USA 77:1078; Henikoff et al. (1981) Nature 283:835; Hollenberg et al. (1981) Curr. Topics Microbiol. Immunol. 96:119; Hollenberg et al. (1979) "The Expression of Bacterial Antibiotic Resistance Genes in the Yeast Saccharomyces cerevisiae," in: Plasmids of Medical, Environmental and Commercial Importance (eds. K.N. Timmis and A. Puhler); Mercerau-Puigalon et al. (1980) Gene 11:163; Panthier et al. (1980) Curr. Genet. 2:109;). A DNA molecule may be expressed inrracellularly in yeast. A promoter sequence may be directly linked with the DNA molecule, in which case the first amino acid at the N- terminus of the recombinant protein will always be a methionine, which is encoded by the ATG start codon. If desired, methionine at the N-terminus may be cleaved from the protein by in vitro incubation with cyanogen bromide. Fusion proteins provide an alternative for yeast expression systems, as well as in mammalian, plant, baculovirus, and bacterial expression systems. Usually, a DNA sequence encoding the N-terminal portion of an endogenous yeast protein, or other stable protein, is fused to the 5' end of heterologous coding sequences. Upon expression, this construct will provide a fusion of the two amino acid sequences. For example, the yeast or human superoxide dismutase (SOD) gene, can be linked at the 5' terminus of a foreign gene and expressed in yeast. The DNA sequence at the junction of the two amino acid sequences may or may not encode a cleavable site. See e.g., EPO Publ. No. 196056. Another example is a ubiquitin fusion protein. Such a fusion protein is made with the ubiquitin region that preferably retains a site for a processing enzyme (e.g. ubiquitin-specific processing protease) to cleave the ubiquitin from the foreign protein. Through this method, therefore, native foreign protein can be isolated (e.g., WO88/024066). Alternatively, foreign proteins can also be secreted from the cell into the growth media by creating chimeric DNA molecules that encode a fusion protein comprised of a leader sequence fragment that provide for secretion in yeast of the foreign protein. Preferably, there are processing sites encoded between the leader fragment and the foreign gene that can be cleaved either in vivo or in vitro. The leader sequence fragment usually encodes a signal peptide comprised of hydrophobic amino acids which direct the secretion of the protein from the cell. DNA encoding suitable signal sequences can be derived from genes for secreted yeast proteins, such as the yeast invertase gene (EPO Publ. No. 012 873; JPO Publ. No. 62:096,086) and the A-factor gene (U.S. Patent 4,588,684). Alternatively, leaders of non- yeast origin, such as an interferon leader, exist that also provide for secretion in yeast (EPO Publ. No. 060 057). A preferred class of secretion leaders are those that employ a fragment of the yeast alpha-factor gene, which contains both a "pre" signal sequence, and a "pro" region. The types of alpha- factor fragments that can be employed include the full-length pre-pro alpha factor leader (about 83 amino acid residues) as well as truncated alpha-factor leaders (usually about 25 to about 50 amino acid residues) (U.S. Patent Nos. 4,546,083 and 4,870,008; EPO Publ. No. 324 274). Additional leaders employing an alpha-factor leader fragment that provides for secretion include hybrid alpha-factor leaders made with a presequence of a first yeast, but a pro-region from a second yeast alphafactor. (See e.g., PCT Publ. No. WO 89/02463.) Usually, transcription termination sequences recognized by yeast are regulatory regions located 3' to the translation stop codon, and thus together with the promoter flank the coding sequence. These sequences direct the transcription of an mRNA which can be translated into the polypeptide encoded by the DNA. Examples of transcription terminator sequence and other yeast-recognized termination sequences, such as those coding for glycolytic enzymes. Usually, the above described components, comprising a promoter, leader (if desired), coding sequence of interest, and transcription termination sequence, are put together into expression constructs. Expression constructs are often maintained in a replicon, such as an extrachromosomal element (e.g., plasmids) capable of stable maintenance in a host, such as yeast or bacteria. The replicon may have two replication systems, thus allowing it to be maintained, for example, in yeast for expression and in a prokaryotic host for cloning and amplification. Examples of such yeast-bacteria shuttle vectors include YEp24 (Botstein et al. (1979) Gene 5:17-24), pCl/1 (Brake et al. (1984) Proc. Natl. Acad. Sci USA 81:4642-4646), and YRpl7 (Stinchcomb et al. (1982) J. Mol. Biol. 158:151). In addition, a replicon may be either a high or low copy number plasmid. A high copy number plasmid will generally have a copy number ranging from about 5 to about 200, and usually about 10 to about 150. A host containing a high copy number plasmid will preferably have at least about 10, and more preferably at least about 20. Enter a high or low copy number vector may be selected, depending upon the effect of the vector and the foreign protein on the host. See e.g., Brake et al, supra. Alternatively, the expression constructs can be integrated into the yeast genome with an integrating vector. Integrating vectors usually contain at least one sequence homologous to a yeast chromosome that allows the vector to integrate, and preferably contain two homologous sequences flanking the expression construct. Integrations appear to result from recombinations between homologous DNA in the vector and the yeast chromosome (Orr- Weaver et al. (1983) Methods in Enzymol. 101:228-245). An integrating vector may be directed to a specific locus in yeast by selecting the appropriate homologous sequence for inclusion in the vector. See Orr- Weaver et al, supra. One or more expression construct may integrate, possibly affecting levels of recombinant protein produced (Rine et al. (1983) Proc. Natl. Acad. Sci. USA 80:6150). The chromosomal sequences included in the vector can occur either as a single segment in the vector, which results in the integration of the entire vector, or two segments homologous to adjacent segments in the chromosome and flanking the expression construct in the vector, which can result in the stable integration of only the expression construct. Usually, extrachromosomal and integrating expression constructs may contain selectable markers to allow for the selection of yeast strains that have been transformed. Selectable markers may include biosynthetic genes that can be expressed in the yeast host, such as ADE2, HIS4, LEU2, TRP1, and ALG7, and the G418 resistance gene, which confer resistance in yeast cells to tunicamycin and G418, respectively. In addition, a suitable selectable marker may also provide yeast with the ability to grow in the presence of toxic compounds, such as metal. For example, the presence of CUP1 allows yeast to grow in the presence of copper ions (Butt et al. (1987) Microbiol, Rev. 57:351). Alternatively, some of the above described components can be put together into transformation vectors. Transformation vectors are usually comprised of a selectable marker that is either maintained in a replicon or developed into an integrating vector, as described above. Expression and transformation vectors, either extrachromosomal replicons or integrating vectors, have been developed for transformation into many yeasts. For example, expression vectors and methods of introducing exogenous DNA into yeast hosts have been developed for, inter alia, the following yeasts: Candida albicans (Kurtz, et al. (1986) Mol. Cell. Biol. 6: 42); Candida maltosa (Kunze, et al. (1985) J. Basic Microbiol. 25:141); Hansenula polymorpha (Gleeson, et al. (1986) J. Gen. Microbiol. 132:3459; Roggenkamp et al. (1986) Mol. Gen. Genet. 202:302); Kluyveromyces fragilis (Das, et al. (1984) J. Bacteriol. 158:1165); Kluyveromyces lactis (De Louvencourt et al. (1983) J Bacteriol. 154:131; Van den Berg et al. (1990) Bio/Technology 5:135); Pichia guillerimondii (Kunze et al. (1985) J. Basic Microbiol. 25:141); Pichia pastoris (Cregg, et al. (1985) Mol. Cell. Biol. 5:3316; U.S. Patent Nos. 4,837,148 and 4,929,555); Saccharomyces cerevisiae (Hinnen et al. (1978) Proc. Natl. Acad. Sci. USA 75:1929; Ito et al. (1983) J. Bacteriol. 153:163); Schizosaccharomyces pombe (Beach and Nurse (1981) Nature 300:106); and Yarrowia lipolytica (Davidow, et al. (1985) Curr. Genet. 10:380411 Gaillardin, et al. (1985) Curr. Genet. 10:49). Methods of introducing exogenous DNA into yeast hosts are well-known in the art, and usually include either the transformation of spheroplasts or of intact yeast cells treated with alkali cations. Transformation procedures usually vary with the yeast species to be transformed. See e.g., [Kurtz et al. (1986) Mol. Cell. Biol. 6:142; Kunze et al. (1985) J Basic Microbiol. 25:141; Candida]; [Gleeson et al. (1986) J. Gen. Microbiol. 732:3459; Roggenkamp et al. (1986) Mol. Gen. Genet. 202:302; Hansenula]; [Das et al. (1984) J. Bacteriol. 755:1165; De Louvencourt et al. (1983) J Bacteriol. 154: 65; Van den Berg et al. (1990) Bio/Technology 5:135; Kluyveromyces]; [Cregg et al. (1985) Mol. Cell. Biol. 5:3316; Kunze et al. (1985) J. Basic Microbiol. 25:141; U.S. Patent Nos. 4,837,148 and 4,929,555; Pichia]; [Hinnen et al. (1978) Proc. Natl. Acad. Sci. USA 75;1929; Ito et al. (1983) J. Bacteriol. 753:163 Saccharomyces]; [Beach and Nurse (1981) Nature 300:106; Schizosaccharomyces]; [Davidow et al. (1985) Curr. Genet. 10:39; Gaillardin et al. (1985) Curr. Genet. 10:49; Yarrowia]. Definitions A composition containing X is "substantially free of Y when at least 85% by weight of the total X+Y in the composition is X. Preferably, X comprises at least about 90% by weight of the total of X+Y in the composition, more preferably at least about 95% > or even 99% by weight. A "conserved" Neisseria amino acid fragment or protein is one that is present in a particular Neisserial protein in at least x% of Neisseria. The value of x may be 50% or more, e.g., 66%, 75%, 80%), 90%, 95% or even 100% (i.e. the amino acid is found in the protein in question in all Neisseria). In order to determine whether an animo acid is "conserved" in a particular Neisserial protein, it is necessary to compare that amino acid residue in the sequences of the protein in question from a plurality of different Neisseria (a reference population). The reference population may include a number of different Neisseria species or may include a single species. The reference population may include a number of different serogroups of a particular species or a single serogroup. A preferred reference population consists of the 5 most common Neisseria strains. The term "heterologous" refers to two biological components that are not found together in nature. The components may be host cells, genes, or regulatory regions, such as promoters. Although the heterologous components are not found together in nature, they can function together, as when a promoter heterologous to a gene is operably linked to the gene. Another example is where a Neisserial sequence is heterologous to a mouse host cell. "Epitope" means antigenic determinant, and may elicit a cellular and/or humoral response. Conditions for "high stringency" are 65 degrees C in 0.1 xSSC 0.5% SDS solution. An "origin of replication" is a polynucleotide sequence that initiates and regulates replication of polynucleotides, such as an expression vector. The origin of replication behaves as an autonomous unit of polynucleotide replication within a cell, capable of replication under its own control. An origin of replication may be needed for a vector to replicate in a particular host cell. With certain origins of replication, an expression vector can be reproduced at a high copy number in the presence of the appropriate proteins within the cell. Examples of origins are the autonomously replicating sequences, which are effective in yeast; and the viral T-antigen, effective in COS-7 cells. A "mutant" sequence is defined as a DNA, RNA or amino acid sequence differing from but having homology with the native or disclosed sequence. Depending on the particular sequence, the degree of homology (sequence identity) between the native or disclosed sequence and the mutant sequence is preferably greater than 50% (e.g., 60%, 70%, 80%, 90%, 95%o, 99% or more) which is calculated as described above. As used herein, an "allelic variant" of a nucleic acid molecule, or region, for which nucleic acid sequence is provided herein is a nucleic acid molecule, or region, that occurs at essentially the same locus in the genome of another or second isolate, and that, due to natural variation caused by, for example, mutation or recombination, has a similar but not identical nucleic acid sequence. A coding region allelic variant typically encodes a protein having similar activity to that of the protein encoded by the gene to which it is being compared. An allelic variant can also comprise an alteration in the 5 ' or 3' untranslated regions of the gene, such as in regulatory control regions, (see, for example, U.S. Patent 5,753,235). Antibodies As used herein, the term "antibody" refers to a polypeptide or group of polypeptides composed of at least one antibody combining site. An "antibody combining site" is the three-dimensional binding space with an internal surface shape and charge distribution complementary to the features of an epitope of an antigen, which allows a binding of the antibody with the antigen. "Antibody" includes, for example, vertebrate antibodies, hybrid antibodies, chimeric antibodies, humanized antibodies, altered antibodies, univalent antibodies, Fab proteins, and single domain antibodies. Antibodies against the proteins of the invention are useful for affinity chromatography, immunoassays, and distinguishing/identifying Neisseria menB proteins. Antibodies elicited against the proteins of the present invention bind to antigenic polypeptides or proteins or protein fragments that are present and specifically associated with strains of Neisseria meningitidis menB. In some instances, these antigens may be associated with specific strains, such as those antigens specific for the menB strains. The antibodies of the invention may be immobilized to a matrix and utilized in an immunoassay or on an affinity chromatography column, to enable the detection and/or separation of polypeptides, proteins or protein fragments or cells comprising such polypeptides, proteins or protein fragments. Alternatively, such polypeptides, proteins or protein fragments may be immobilized so as to detect antibodies bindably specific thereto. Antibodies to the proteins of the invention, both polyclonal and monoclonal, may be prepared by conventional methods. In general, the protein is first used to immunize a suitable animal, preferably a mouse, rat, rabbit or goat. Rabbits and goats are preferred for the preparation of polyclonal sera due to the volume of serum obtainable, and the availability of labeled anti-rabbit and anti-goat antibodies. Immunization is generally performed by mixing or emulsifying the protein in saline, preferably in an adjuvant such as Freund's complete adjuvant, and injecting the mixture or emulsion parenterally (generally subcutaneously or intramuscularly). A dose of 50-200 μg/injection is typically sufficient. Immunization is generally boosted 2-6 weeks later with one or more injections of the protein in saline, preferably using Freund's incomplete adjuvant. One may alternatively generate antibodies by in vitro immunization using methods known in the art, which for the purposes of this invention is considered equivalent to in vivo immunization. Polyclonal antisera is obtained by bleeding the immunized animal into a glass or plastic container, incubating the blood at 25°C for one hour, followed by incubating at 4°C for 2-18 hours. The serum is recovered by centrifugation (e.g., l,000g for 10 minutes). About 20-50 ml per bleed may be obtained from rabbits. Monoclonal antibodies are prepared using the standard method of Kohler & Milstein (Nature (1975) 256:495-96), or a modification thereof. Typically, a mouse or rat is immunized as described above. However, rather than bleeding the animal to extract serum, the spleen (and optionally several large lymph nodes) is removed and dissociated into single cells. If desired, the spleen cells may be screened (after removal of nonspecifically adherent cells) by applying a cell suspension to a plate or well coated with the protein antigen. B-cells that express membrane-bound immunoglobulin specific for the antigen bind to the plate, and are not rinsed away with the rest of the suspension. Resulting B-cells, or all dissociated spleen cells, are then induced to fuse with myeloma cells to form hybridomas, and are cultured in a selective medium (e.g., hypoxanthine, aminopterin, thymidine medium, "HAT"). The resulting hybridomas are plated by limiting dilution, and are assayed for the production of antibodies which bind specifically to the immunizing antigen (and which do not bind to unrelated antigens). The selected MAb-secreting hybridomas are then cultured either in vitro (e.g., in tissue culture bottles or hollow fiber reactors), or in vivo (as ascites in mice). If desired, the antibodies (whether polyclonal or monoclonal) may be labeled using conventional techniques. Suitable labels include fluorophores, chromophores, radioactive atoms (particularly 32 P and 125 I), electron-dense reagents, enzymes, and ligands having specific binding partners. Enzymes are typically detected by their activity. For example, horseradish peroxidase is usually detected by its ability to convert 3,3',5,5'-tetramethylbenzidine (TMB) to a blue pigment, quantifiable with a spectrophotometer. "Specific binding partner" refers to a protein capable of binding a ligand molecule with high specificity, as for example in the case of an antigen and a monoclonal antibody specific therefor. Other specific binding partners include biotin and avidin or streptavidin, IgG and protein A, and the numerous receptor-ligand couples known in the art. It should be understood that the above description is not meant to categorize the various labels into distinct classes, as the same label may serve in several different modes. For example, 125 I may serve as a radioactive label or as an electron-dense reagent. HRP may serve as enzyme or as antigen for a MAb. Further, one may combine various labels for desired effect. For example, MAbs and avidin also require labels in the practice of this invention: thus, one might label a MAb with biotin, and detect its presence with avidin labeled with 125 I, or with an anti-biotin MAb labeled with HRP. Other permutations and possibilities will be readily apparent to those of ordinary skill in the art, and are considered as equivalents within the scope of the instant invention. Antigens, immunogens, polypeptides, proteins or protein fragments of the present invention elicit formation of specific binding partner antibodies. These antigens, immunogens, polypeptides, proteins or protein fragments of the present invention comprise immunogenic compositions of the present invention. Such immunogenic compositions may further comprise or include adjuvants, carriers, or other compositions that promote or enhance or stabilize the antigens, polypeptides, proteins or protein fragments of the present invention. Such adjuvants and carriers will be readily apparent to those of ordinary skill in the art. Pharmaceutical Compositions Pharmaceutical compositions can comprise (include) either polypeptides, antibodies, or nucleic acid of the invention. The pharmaceutical compositions will comprise a therapeutically effective amount of either polypeptides, antibodies, or polynucleotides of the claimed invention. The term "therapeutically effective amount" as used herein refers to an amount of a therapeutic agent to treat, ameliorate, or prevent a desired disease or condition, or to exhibit a detectable therapeutic or preventative effect. The effect can be detected by, for example, chemical markers or antigen levels. Therapeutic effects also include reduction in physical symptoms, such as decreased body temperature, when given to a patient that is febrile. The precise effective amount for a subject will depend upon the subject's size and health, the nature and extent of the condition, and the therapeutics or combination of therapeutics selected for administration. Thus, it is not useful to specify an exact effective amount in advance. However, the effective amount for a given situation can be determined by routine experimentation and is within the judgment of the clinician. For purposes of the present invention, an effective dose will be from about 0.01 mg/ kg to 50 mg/kg or 0.05 mg/kg to about 10 mg/kg of the DNA constructs in the individual to which it is administered. A pharmaceutical composition can also contain a pharmaceutically acceptable carrier. The term "pharmaceutically acceptable carrier" refers to a carrier for administration of a therapeutic agent, such as antibodies or a polypeptide, genes, and other therapeutic agents. The term refers to any pharmaceutical carrier that does not itself induce the production of antibodies harmful to the individual receiving the composition, and which may be administered without undue toxicity. Suitable carriers may be large, slowly metabolized macromolecules such as proteins, polysaccharides, polylactic acids, polyglycolic acids, polymeric amino acids, amino acid copolymers, and inactive virus particles. Such carriers are well known to those of ordinary skill in the art. Pharmaceutically acceptable salts can be used therein, for example, mineral acid salts such as hydrochlorides, hydrobromides, phosphates, sulfates, and the like; and the salts of organic acids such as acetates, propionates, malonates, benzoates, and the like. A thorough discussion of pharmaceutically acceptable excipients is available in Remington's Pharmaceutical Sciences (Mack Pub. Co., N.J. 1991). Pharmaceutically acceptable carriers in therapeutic compositions may contain liquids such as water, saline, glycerol and ethanol. Additionally, auxiliary substances, such as wetting or emulsifying agents, pH buffering substances, and the like, may be present in such vehicles. Typically, the therapeutic compositions are prepared as injectables, either as liquid solutions or suspensions; solid forms suitable for solution in, or suspension in, liquid vehicles prior to injection may also be prepared. Liposomes are included within the definition of a pharmaceutically acceptable carrier. Delivery Methods Once formulated, the compositions of the invention can be administered directly to the subject. The subjects to be treated can be animals; in particular, human subjects can be treated. Direct delivery of the compositions will generally be accomplished by injection, either subcutaneously, intraperitoneally, intravenously or intramuscularly or delivered to the interstitial space of a tissue. The compositions can also be administered into a lesion. Other modes of administration include oral and pulmonary administration, suppositories, and transdermal and transcutaneous applications, needles, and gene guns or hyposprays. Dosage treatment may be a single dose schedule or a multiple dose schedule. Vaccines Vaccines according to the invention may either be prophylactic (i.e., to prevent infection) or therapeutic (i.e., to treat disease after infection). Such vaccines comprise immunizing antigen(s) or immunogen(s), immunogenic polypeptide, protein(s) or protein fragments, or nucleic acids (e.g., ribonucleic acid or deoxyribonucleic acid), usually in combination with "pharmaceutically acceptable carriers," which include any carrier that does not itself induce the production of antibodies harmful to the individual receiving the composition. Suitable carriers are typically large, slowly metabolized macromolecules such as proteins, polysaccharides, polylactic acids, polyglycolic acids, polymeric amino acids, amino acid copolymers, lipid aggregates (such as oil droplets or liposomes), and inactive virus particles. Such carriers are well known to those of ordinary skill in the art. Additionally, these carriers may function as immunostimulating agents ("adjuvants"). Furthermore, the immunogen or antigen may be conjugated to a bacterial toxoid, such as a toxoid from diphtheria, tetanus, cholera, 77. pylori, etc. pathogens. Preferred adjuvants to enhance effectiveness of the composition include, but are not limited to: (1) aluminum salts (alum), such as aluminum hydroxide, aluminum phosphate, aluminum sulfate, etc; (2) oil-in- water emulsion formulations (with or without other specific immunostimulating agents such as muramyl peptides (see below) or bacterial cell wall components), such as for example (a) MF59 (PCT Publ. No. WO 90/14837), containing 5% Squalene, 0.5% Tween 80, and 0.5% Span 85 (optionally containing various amounts of MTP-PE (see below), although not required) formulated into submicron particles using a microfluidizer such as Model HOY micro fluidizer (Micro fluidics, Newton, MA), (b) SAF, containing 10% Squalane, 0.4% Tween 80, 5% pluronic-blocked polymer L121, and thr-MDP (see below) either microfluidized into a submicron emulsion or vortexed to generate a larger particle size emulsion, and (c) Ribi™ adjuvant system (RAS), (Ribi Immunochem, Hamilton, MT) containing 2% Squalene, 0.2% Tween 80, and one or more bacterial cell wall components from the group consisting of monophosphorylipid A (MPL), trehalose dimycolate (TDM), and cell wall skeleton (CWS), preferably MPL + CWS (Detox™); (3) saponin adjuvants, such as Stimulon™ (Cambridge Bioscience, Worcester, MA) may be used or particles generated therefrom such as ISCOMs (immunostimulating complexes); (4) Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA); (5) cytokines, such as interleukins (e.g., IL-1, IL-2, IL-4, IL-5, IL-6, IL-7, IL-12, etc.), interferons (e.g., gamma interferon), macrophage colony stimulating factor (M-CSF), tumor necrosis factor (TNF), etc; (6) detoxified mutants of a bacterial ADP-ribosylating toxin such as a cholera toxin (CT), a pertussis toxin (PT), or an E. coli heat-labile toxin (LT), particularly LT-K63, LT-R72, CT-S109, PT-K9/G129; see, e.g., WO 93/13302 and WO 92/19265; and (7) other substances that act as immunostimulating agents to enhance the effectiveness of the composition. Alum and MF59 are preferred. As mentioned above, muramyl peptides include, but are not limited to, N-acetyl- muramyl-L-threonyl-D-isoglutamine (thr-MDP), N-acetyl-normuramyl-L-alanyl-D- isoglutamine (nor-MDP), N-acetylmuramyl-L-alanyl-D-isoglutaminyl-L-alanine-2-(r-2'- dipalmitoyl-5«-glycero-3-huydroxyphosphoryloxy)-ethylamine (MTP-PE), etc. The vaccine compositions comprising immunogenic compositions (e.g., which may include the antigen, pharmaceutically acceptable carrier, and adjuvant) typically will contain diluents, such as water, saline, glycerol, ethanol, etc. Additionally, auxiliary substances, such as wetting or emulsifying agents, pH buffering substances, and the like, may be present in such vehicles. Alternatively, vaccine compositions comprising immunogenic compositions may comprise an antigen, polypeptide, protein, protein fragment or nucleic acid in a pharmaceutically acceptable carrier. More specifically, vaccines comprising immunogenic compositions comprise an immuno logically effective amount of the immunogenic polypeptides, as well as any other of the above-mentioned components, as needed. By "immunologically effective amount", it is meant that the administration of that amount to an individual, either in a single dose or as part of a series, is effective for treatment or prevention. This amount varies depending upon the health and physical condition of the individual to be treated, the taxonomic group of individual to be treated (e.g., nonhuman primate, primate, etc.), the capacity of the individual's immune system to synthesize antibodies, the degree of protection desired, the formulation of the vaccine, the treating doctor's assessment of the medical situation, and other relevant factors. It is expected that the amount will fall in a relatively broad range that can be determined through routine trials. Typically, the vaccine compositions or immunogenic compositions are prepared as injectables, either as liquid solutions or suspensions; solid forms suitable for solution in, or suspension in, liquid vehicles prior to injection may also be prepared. The preparation also may be emulsified or encapsulated in liposomes for enhanced adjuvant effect, as discussed above under pharmaceutically acceptable carriers. The immunogenic compositions are conventionally administered parenterally, e.g., by injection, either subcutaneously or intramuscularly. Additional formulations suitable for other modes of administration include oral and pulmonary formulations, suppositories, and transdermal and transcutaneous applications. Dosage treatment may be a single dose schedule or a multiple dose schedule. The vaccine may be administered in conjunction with other immunoregulatory agents. As an alternative to protein-based vaccines, DNA vaccination may be employed (e.g., Robinson & Torres (1997) Seminars in Immunology 9:271-283; Donnelly et al. (1997) Annu Rev Immunol 15:617-648). Gene Delivery Vehicles Gene therapy vehicles for delivery of constructs, including a coding sequence of a therapeutic of the invention, to be delivered to the mammal for expression in the mammal, can be administered either locally or systemically. These constructs can utilize viral or non- viral vector approaches in in vivo or ex vivo modality. Expression of such coding sequence can be induced using endogenous mammalian or heterologous promoters. Expression of the coding sequence in vivo can be either constitutive or regulated. The invention includes gene delivery vehicles capable of expressing the contemplated nucleic acid sequences. The gene delivery vehicle is preferably a viral vector and, more preferably, a retroviral, adenoviral, adeno-associated viral (AAV), herpes viral, or alphavirus vector. The viral vector can also be an astrovirus, coronavirus, orthomyxovirus, papovavirus, paramyxovirus, parvovirus, picornavirus, poxvirus, or togavirus viral vector. See generally, Jolly (1994) Cancer Gene Therapy 1 :51-64; Kimura (1994) Human Gene Therapy 5:845-852; Connelly (1995) Human Gene Therapy 6:185-193; and Kaplitt (1994) Nαtwre Genetics 6:148-153. Retroviral vectors are well known in the art, including B, C and D type retroviruses, xenotropic retroviruses (for example, ΝZB-X1, ΝZB-X2 and NZB9-1 (see O'Neill (1985) J. Virol. 53:160) polytropic retroviruses e.g., MCF and MCF-MLV (see Kelly (1983) J. Virol. 45:291), spumaviruses and lentiviruses. See RNA Tumor Viruses, Second Edition, Cold Spring Harbor Laboratory, 1985. Portions of the retroviral gene therapy vector may be derived from different retroviruses. For example, retrovector LTRs may be derived from a Murine Sarcoma Virus, a tRNA binding site from a Rous Sarcoma Virus, a packaging signal from a Murine Leukemia Virus, and an origin of second strand synthesis from an Avian Leukosis Virus. These recombinant retroviral vectors may be used to generate transduction competent retroviral vector particles by introducing them into appropriate packaging cell lines (see US patent 5,591,624). Retrovirus vectors can be constructed for site-specific integration into host cell DNA by incorporation of a chimeric integrase enzyme into the retroviral particle (see WO96/37626). It is preferable that the recombinant viral vector is a replication defective recombinant virus. Packaging cell lines suitable for use with the above-described retrovirus vectors are well known in the art, are readily prepared (see WO95/30763 and WO92/05266), and can be used to create producer cell lines (also termed vector cell lines or "VCLs") for the production of recombinant vector particles. Preferably, the packaging cell lines are made from human parent cells (e.g., HT1080 cells) or mink parent cell lines, which eliminates inactivation in human serum. Preferred retroviruses for the construction of retroviral gene therapy vectors include Avian Leukosis Virus, Bovine Leukemia, Virus, Murine Leukemia Virus, Mink-Cell Focus-Inducing Virus, Murine Sarcoma Virus, Reticuloendotheliosis Virus and Rous Sarcoma Virus. Particularly preferred Murine Leukemia Viruses include 4070A and 1504A (Hartley and Rowe (1976) J Virol 19:19-25), Abelson (ATCC No. VR-999), Friend (ATCC No. VR-245), Graffi, Gross (ATCC Nol VR-590), Kirsten, Harvey Sarcoma Virus and Rauscher (ATCC No. VR-998) and Moloney Murine Leukemia Virus (ATCC No. VR-190). Such retroviruses may be obtained from depositories or collections such as the American Type Culture Collection ("ATCC") in Rockville, Maryland or isolated from known sources using commonly available techniques. Exemplary known retroviral gene therapy vectors employable in this invention include those described in patent applications GB2200651, EP0415731, EP0345242, EP0334301, WO89/02468; WO89/05349, WO89/09271, WO90/02806, WO90/07936, WO94/03622, WO93/25698, WO93/25234, WO93/11230, WO93/10218, WO91/02805, WO91/02825, WO95/07994, US 5,219,740, US 4,405,712, US 4,861,719, US 4,980,289, US 4,777,127, US 5,591,624. See also Vile (1993) Cancer Res 53:3860-3864; Vile (1993) Cancer Res 53:962-967; Ram (1993) Cancer Res 53 (1993) 83-88; Takamiya (1992) JNewrøsct Res 33:493-503; Baba (1993) JNeurosurg 79:729-735; Mann (1983) Cell 33:153; Cane (1984) Proc Natl Acad Sci 81:6349; and Miller (1990) Human Gene Therapy 1. Human adenoviral gene therapy vectors are also known in the art and employable in this invention. See, for example, Berkner (1988) Biotechniques 6:616 and Rosenfeld (1991) Science 252:431, and WO93/07283, WO93/06223, and WO93/07282. Exemplary known adenoviral gene therapy vectors employable in this invention include those described in the above referenced documents and in WO94/12649, WO93/03769, WO93/19191, WO94/28938, WO95/11984, WO95/00655, WO95/27071, WO95/29993, WO95/34671, WO96/05320, WO94/08026, WO94/11506, WO93/06223, WO94/24299, WO95/14102, WO95/24297, WO95/02697, WO94/28152, WO94/24299, WO95/09241, WO95/25807, WO95/05835, WO94/18922 and WO95/09654. Alternatively, administration of DNA linked to killed adenovirus as described in Curiel (1992) Hum. Gene Ther. 3:147-154 may be employed. The gene delivery vehicles of the invention also include adenovirus associated virus (AAV) vectors. Leading and preferred examples of such vectors for use in this invention are the AAV-2 based vectors disclosed in Srivastava, WO93/09239. Most preferred AAV vectors comprise the two AAV inverted terminal repeats in which the native D-sequences are modified by substitution of nucleotides, such that at least 5 native nucleotides and up to 18 native nucleotides, preferably at least 10 native nucleotides up to 18 native nucleotides, most preferably 10 native nucleotides are retained and the remaining nucleotides of the D-sequence are deleted or replaced with non-native nucleotides. The native D-sequences of the AAV inverted terminal repeats are sequences of 20 consecutive nucleotides in each AAV inverted terminal repeat (i.e., there is one sequence at each end) which are not involved in HP formation. The non-native replacement nucleotide may be any nucleotide other than the nucleotide found in the native D-sequence in the same position. Other employable exemplary AAV vectors are pWP-19, pWN-1, both of which are disclosed in Nahreini (1993) Gene 124:257-262. Another example of such an AAV vector is psub201 (see Samulski (1987) J. Virol. 61:3096). Another exemplary AAV vector is the Double-D ITR vector. Construction of the Double-D ITR vector is disclosed in US Patent 5,478,745. Still other vectors are those disclosed in Carter US Patent 4,797,368 and Muzyczka US Patent 5,139,941, Chartejee US Patent 5,474,935, and Kotin WO94/288157. Yet a further example of an AAV vector employable in this invention is SSV9AFABTKneo, which contains the AFP enhancer and albumin promoter and directs expression predominantly in the liver. Its structure and construction are disclosed in Su (1996) Human Gene Therapy 7:463-470. Additional AAV gene therapy vectors are described in US 5,354,678, US 5,173,414, US 5,139,941, and US 5,252,479. The gene therapy vectors comprising sequences of the invention also include herpes vectors. Leading and preferred examples are herpes simplex virus vectors containing a sequence encoding a thymidine kinase polypeptide such as those disclosed in US 5,288,641 and EPO 176170 (Roizman). Additional exemplary herpes simplex virus vectors include HFEM/ICP6-LacZ disclosed in WO95/04139 (Wistar Institute), pHSVlac described in Geller (1988) Science 241 :1667-1669 and in WO90/09441 and WO92/07945, HSV Us3::pgC-lacZ described in Fink (1992) Human Gene Therapy 3:11-19 and HSV 7134, 2 RH 105 and GAL4 described in EP 0453242 (Breakefield), and those deposited with the ATCC as accession numbers ATCC VR-977 and ATCC VR-260. Also contemplated are alpha virus gene therapy vectors that can be employed in this invention. Preferred alpha virus vectors are Sindbis viruses vectors. Togaviruses, Semliki Forest virus (ATCC VR-67; ATCC VR-1247), Middleberg virus (ATCC VR-370), Ross River virus (ATCC VR-373; ATCC VR-1246), Venezuelan equine encephalitis virus (ATCC VR923; ATCC VR-1250; ATCC VR-1249; ATCC VR-532), and those described in US patents 5,091,309, 5,217,879, and WO92/10578. More particularly, those alpha virus vectors described in U.S. Serial No. 08/405,627, filed March 15, 1995,WO94/21792, WO92/10578, WO95/07994, US 5,091,309 and US 5,217,879 are employable. Such alpha viruses may be obtained from depositories or collections such as the ATCC in Rockville, Maryland or isolated from known sources using commonly available techniques. Preferably, alphavirus vectors with reduced cytotoxicity are used (see USSN 08/679640). DNA vector systems such as eukarytic layered expression systems are also useful for expressing the nucleic acids of the invention. SeeWO95/07994 for a detailed description of eukaryotic layered expression systems. Preferably, the eukaryotic layered expression systems of the invention are derived from alphavirus vectors and most preferably from Sindbis viral vectors. Other viral vectors suitable for use in the present invention include those derived from poliovirus, for example ATCC VR-58 and those described in Evans, Nature 339 (1989) 385 and Sabin ( 1973) J. Biol. Standardization 1:115; rhinovirus, for example ATCC VR- 1110 and those described in Arnold (1990) J Cell Biochem L401 ; pox viruses such as canary pox virus or vaccinia virus, for example ATCC VR-111 and ATCC VR-2010 and those described in Fisher-Hoch (1989) Proc Natl Acad Sci 86:317; Flexner (1989) Ann NY Acad Sci 569:86, Flexner (1990) Vaccine 8:17; in US 4,603,112 and US 4,769,330 and WO89/01973; SV40 virus, for example ATCC VR-305 and those described in Mulligan (1979) Nαtwre 277:108 and Madzak (1992) J Gen Virol 73:1533; influenza virus, for example ATCC VR-797 and recombinant influenza viruses made employing reverse genetics techniques as described in US 5,166,057 and in Enami (1990) Proc Natl Acad Sci 87:3802-3805; Enami & Palese (1991) J Virol 65:2711-2713 and Luytjes (1989) Cell 59:110, (see also McMichael (1983) NEJMed 309:13, and Yap (1978) Nature 273:238 and Nature (1979) 277:108); human immunodeficiency virus as described in EP-0386882 and in Buchschacher (1992) J. Virol. 66:2731; measles virus, for example ATCC VR-67 and VR-1247 and those described in EP- 0440219; Aura virus, for example ATCC VR-368; Bebaru virus, for example ATCC VR-600 and ATCC VR-1240; Cabassou virus, for example ATCC VR-922; Chikungunya virus, for example ATCC VR-64 and ATCC VR-1241; Fort Morgan Virus, for example ATCC VR-924; Getah virus, for example ATCC VR-369 and ATCC VR-1243; Kyzylagach virus, for example ATCC VR-927; Mayaro virus, for example ATCC VR-66; Mucambo virus, for example ATCC VR-580 and ATCC VR-1244; Ndumu virus, for example ATCC VR-371; Pixuna virus, for example ATCC VR-372 and ATCC VR-1245; Tonate virus, for example ATCC VR-925; Triniti virus, for example ATCC VR-469; Una virus, for example ATCC VR-374; Whataroa virus, for example ATCC VR-926; Y-62-33 virus, for example ATCC VR-375; O'Nyong virus, Eastern encephalitis virus, for example ATCC VR-65 and ATCC VR-1242; Western encephalitis virus, for example ATCC VR-70, ATCC VR-1251, ATCC VR-622 and ATCC VR-1252; and coronavirus, for example ATCC VR-740 and those described in Hamre (1966) Proc Soc Exp Biol Med 121:190. Delivery of the compositions of this invention into cells is not limited to the above mentioned viral vectors. Other delivery methods and media may be employed such as, for example, nucleic acid expression vectors, polycationic condensed DNA linked or unlinked to killed adenovirus alone, for example see US Serial No. 08/366,787, filed December 30, 1994 and Curiel (1992) Hum Gene Ther 3:147-154 ligand linked DNA, for example see Wu (1989) JBiol Chem 264:16985-16987, eucaryotic cell delivery vehicles cells, for example see US Serial No.08/240,030, filed May 9, 1994, and US Serial No. 08/404,796, deposition of photopolymerized hydrogel materials, hand-held gene transfer particle gun, as described in US Patent 5,149,655, ionizing radiation as described in US5,206,152 and in WO92/11033, nucleic charge neutralization or fusion with cell membranes. Additional approaches are described in Philip (1994) Mol Cell Biol 14:2411-2418 and in Woffendin (1994) Proc Natl Acad Sci 91:1581-1585. Particle mediated gene transfer may be employed, for example see US Serial No. 60/023,867. Briefly, the sequence can be inserted into conventional vectors that contain conventional control sequences for high level expression, and then incubated with synthetic gene transfer molecules such as polymeric DNA-binding cations like polylysine, protamine, and albumin, linked to cell targeting ligands such as asialoorosomucoid, as described in Wu & Wu (1987) J. Biol. Chem. 262:4429-4432, insulin as described in Hucked (1990) Biochem Pharmacol 40:253-263, galactose as described in Plank (1992) Bioconjugate Chem 3:533-539, lactose or transferrin. Naked DNA may also be employed to transform a host cell. Exemplary naked DNA introduction methods are described in WO 90/11092 and US 5,580,859. Uptake efficiency may be improved using biodegradable latex beads. DNA coated latex beads are efficiently transported into cells after endocytosis initiation by the beads. The method may be improved further by treatment of the beads to increase hydrophobicity and thereby facilitate disruption of the endosome and release of the DNA into the cytoplasm. Liposomes that can act as gene delivery vehicles are described in U.S. 5,422,120, WO95/13796, WO94/23697, WO91/14445 and EP-524,968. As described in USSN. 60/023,867, on non-viral delivery, the nucleic acid sequences encoding a polypeptide can be inserted into conventional vectors that contain conventional control sequences for high level expression, and then be incubated with synthetic gene transfer molecules such as polymeric DNA-binding cations like polylysine, protamine, and albumin, linked to cell targeting ligands such as asialoorosomucoid, insulin, galactose, lactose, or transferrin. Other delivery systems include the use of liposomes to encapsulate DNA comprising the gene under the control of a variety of tissue-specific or ubiquitously-active promoters. Further non- viral delivery suitable for use includes mechanical delivery systems such as the approach described in Woffendin et al (1994) Proc. Natl. Acad. Sci. USA 91(24):11581-11585. Moreover, the coding sequence and the product of expression of such can be delivered through deposition of photopolymerized hydrogel materials. Other conventional methods for gene delivery that can be used for delivery of the coding sequence include, for example, use of hand-held gene transfer particle gun, as described in U.S. 5,149,655; use of ionizing radiation for activating transferred gene, as described in U.S. 5,206,152 and WO92/11033. Exemplary liposome and polycationic gene delivery vehicles are those described in US 5,422,120 and 4,762,915; inWO 95/13796; WO94/23697; and WO91/14445; in EP- 0524968; and in Stryer, Biochemistry, pages 236-240 (1975) W.H. Freeman, San Francisco; Szoka (1980) Biochem Biophys Acta 600:1; Bayer (1979) Biochem Biophys Acta 550:464; Rivnay (1987) Meth Enzymol 149:119; Wang (1987) Proc Natl Acad Sci 84:7851; Plant (1989) Anal Biochem 176:420. A polynucleotide composition can comprises therapeutically effective amount of a gene therapy vehicle, as the term is defined above. For purposes of the present invention, an effective dose will be from about 0.01 mg/ kg to 50 mg/kg or 0.05 mg/kg to about 10 mg/kg of the DNA constructs in the individual to which it is administered. Delivery Methods Once formulated, the polynucleotide compositions of the invention can be administered (1) directly to the subject; (2) delivered ex vivo, to cells derived from the subject; or (3) in vitro for expression of recombinant proteins. The subjects to be treated can be mammals or birds. Also, human subjects can be treated. Direct delivery of the compositions will generally be accomplished by injection, either subcutaneously, intraperitoneally, intravenously or intramuscularly or delivered to the interstitial space of a tissue. The compositions can also be administered into a tumor or lesion. Other modes of administration include oral and pulmonary administration, suppositories, and transdermal applications, needles, and gene guns or hyposprays. Dosage treatment may be a single dose schedule or a multiple dose schedule. Methods for the ex vivo delivery and reimplantation of transformed cells into a subject are known in the art and described in eg. WO93/ 14778. Examples of cells useful in ex vivo applications include, for example, stem cells, particularly hematopoetic, lymph cells, macrophages, dendritic cells, or tumor cells. Generally, delivery of nucleic acids for both ex vivo and in vitro applications can be accomplished by the following procedures, for example, dextran-mediated transfection, calcium phosphate precipitation, polybrene mediated transfection, protoplast fusion, electroporation, encapsulation of the polynucleotide(s) in liposomes, and direct micro injection of the DNA into nuclei, all well known in the art. Polynucleotide and polypeptide pharmaceutical compositions In addition to the pharmaceutically acceptable carriers and salts described above, the following additional agents can be used with polynucleotide and/or polypeptide compositions. A.Polypeptides One example are polypeptides which include, without limitation: asioloorosomucoid (ASOR); transferrin; asialoglycoproteins; antibodies; antibody fragments; ferritin; interleukins; interferons, granulocyte, macrophage colony stimulating factor (GM-CSF), granulocyte colony stimulating factor (G-CSF), macrophage colony stimulating factor (M-CSF), stem cell factor and erythropoietin. Viral antigens, such as envelope proteins, can also be used. Also, proteins from other invasive organisms, such as the 17 amino acid peptide from the circumsporozoite protein of plasmodium falciparum known as RII. B.Hormones, Vitamins, Etc. Other groups that can be included are, for example: hormones, steroids, androgens, estrogens, thyroid hormone, or vitamins, folic acid. C.Polyalkylenes, Polysaccharides, etc. Also, polyalkylene glycol can be included with the desired polynucleotides or polypeptides. In a preferred embodiment, the polyalkylene glycol is polyethlylene glycol. In addition, mono-, di-, or polysaccarides can be included. In a preferred embodiment of this aspect, the polysaccharide is dextran or DEAE-dextran. Also, chitosan and poly(lactide-co-glycolide) D.Lipids, and Liposomes The desired polynucleotide or polypeptide can also be encapsulated in lipids or packaged in liposomes prior to delivery to the subject or to cells derived therefrom. Lipid encapsulation is generally accomplished using liposomes which are able to stably bind or entrap and retain nucleic acid. The ratio of condensed polynucleotide or polypeptide to lipid preparation can vary but will generally be around 1:1 (mg DNA:micromoles lipid), or more of lipid. For a review of the use of liposomes as carriers for delivery of nucleic acids, see, Hug and Sleight (1991) Biochim. Biophys. Acta. 1097:1-17; Straubinger (1983) et/z. Enzymol. 101:512-527. Liposomal preparations for use in the present invention include cationic (positively charged), anionic (negatively charged) and neutral preparations. Cationic liposomes have been shown to mediate intracellular delivery of plasmid DNA (Feigner (1987) Proc. Natl. Acad. Sci. USA 84:7413-7416); mRNA (Malone (1989) Proc. Natl. Acad. Sci. USA 86:6077-6081); and purified transcription factors (Debs (1990) J. Biol. Chem. 265:10189-10192), in functional form. Cationic liposomes are readily available. For example, N[l-2,3-dioleyloxy)propyl]-N,N,N-triethylammonium (DOTMA) liposomes are available under the trademark Lipofectin, from GIBCO BRL, Grand Island, NY. (See, also, Feigner supra). Other commercially available liposomes include transfectace (DDAB/DOPE) and DOTAP/DOPE (Boerhinger). Other cationic liposomes can be prepared from readily available materials using techniques well known in the art. See, eg. Szoka (1978) Proc. Natl. Acad. Sci. USA 75:4194-4198; WO90/11092 for a description of the synthesis of DOTAP (1 ,2-bis(oleoyloxy)-3-(trimethylammonio)propane) liposomes. Similarly, anionic and neutral liposomes are readily available, such as from Avanti Polar Lipids (Birmingham, AL), or can be easily prepared using readily available materials. Such materials include phosphatidyl choline, cholesterol, phosphatidyl ethanolamine, dioleoylphosphatidyl choline (DOPC), dioleoylphosphatidyl glycerol (DOPG), dioleoylphoshatidyl ethanolamine (DOPE), among others. These materials can also be mixed with the DOTMA and DOTAP starting materials in appropriate ratios. Methods for making liposomes using these materials are well known in the art. The liposomes can comprise multilammelar vesicles (MLVs), small unilamellar vesicles (SUVs), or large unilamellar vesicles (LUVs). The various liposome-nucleic acid complexes are prepared using methods known in the art. See eg. Straubinger (1983) Meth. Immunol. 101:512-527; Szoka (1978) Proc. Natl. Acad. Sci. USA 75:4194-4198; Papahadjopoulos (1975) Biochim. Biophys. Acta 394:483; Wilson (1979) Cell 17:77); Deamer & Bangham (1976) Biochim. Biophys. Acta 443:629; Ostro (1977) Biochem. Biophys. Res. Commun. 76:836; Fraley (1979) Proc. Natl. Acad. Sci. USA 76:3348); Enoch & Strittmatter (1979) Proc. Natl. Acad. Sci. USA 76:145; Fraley (1980) J. Biol. Chem. (1980) 255:10431; Szoka & Papahadjopoulos (1978) Proc. Natl. Acad. Sci. USA 75:145; and Schaefer-Ridder (1982) Science 215:166. E.Lipoproteins In addition, lipoproteins can be included with the polynucleotide or polypeptide to be delivered. Examples of lipoproteins to be utilized include: chylomicrons, HDL, IDL, LDL, and VLDL. Mutants, fragments, or fusions of these proteins can also be used. Also, modifications of naturally occurring lipoproteins can be used, such as acetylated LDL. These lipoproteins can target the delivery of polynucleotides to cells expressing lipoprotein receptors. Preferably, if lipoproteins are including with the polynucleotide to be delivered, no other targeting ligand is included in the composition. Naturally occurring lipoproteins comprise a lipid and a protein portion. The protein portion are known as apoproteins. At the present, apoproteins A, B, C, D, and E have been isolated and identified. At least two of these contain several proteins, designated by Roman numerals, Al, All, AIV; CI, CII, CIII. A lipoprotein can comprise more than one apoprotein. For example, naturally occurring chylomicrons comprises of A, B, C, and E, over time these lipoproteins lose A and acquire C and E apoproteins. VLDL comprises A, B, C, and E apoproteins, LDL comprises apoprotein B; and HDL comprises apoproteins A, C, and E. The amino acid of these apoproteins are known and are described in, for example, Breslow (1985) Annu Rev. Biochem 54:699; Law (1986) Adv. Exp Med. Biol. 151:162; Chen (1986) J Biol Chem 261 :12918; Kane (1980) Proc Natl Acad Sci USA 77:2465; and Utermann (1984) Hum Genet 65:232. Lipoproteins contain a variety of lipids including, triglycerides, cholesterol (free and esters), and phopholipids. The composition of the lipids varies in naturally occurring lipoproteins. For example, chylomicrons comprise mainly triglycerides. A more detailed description of the lipid content of naturally occurring lipoproteins can be found, for example, in Meth. Enzymol. 128 (1986). The composition of the lipids are chosen to aid in conformation of the apoprotein for receptor binding activity. The composition of lipids can also be chosen to facilitate hydrophobic interaction and association with the polynucleotide binding molecule. Naturally occurring lipoproteins can be isolated from serum by ultracentrifugation, for instance. Such methods are described in Meth. Enzymol. (supra); Pitas (1980) J. Biochem. 255:5454-5460 and Mahey (1979) J Clin. Invest 64:743-750. Lipoproteins can also be produced by in vitro or recombinant methods by expression of the apoprotein genes in a desired host cell. See, for example, Atkinson (1986) Annu Rev Biophys Chem 15:403 and Radding (1958) Biochim Biophys Acta 30: 443. Lipoproteins can also be purchased from commercial suppliers, such as Biomedical Techniologies, Inc., Stoughton, Massachusetts, USA. Further description of lipoproteins can be found in Zuckermann et al, PCT. Appln. No. US97/14465. F.Polycationic Agents Polycationic agents can be included, with or without lipoprotein, in a composition with the desired polynucleotide or polypeptide to be delivered. Polycationic agents, typically, exhibit a net positive charge at physiological relevant pH and are capable of neutralizing the electrical charge of nucleic acids to facilitate delivery to a desired location. These agents have both in vitro, ex vivo, and in vivo applications. Polycationic agents can be used to deliver nucleic acids to a living subject either intramuscularly, subcutaneously, etc. The following are examples of useful polypeptides as polycationic agents: polylysine, polyarginine, polyornithine, and protamine. Other examples include histones, protamines, human serum albumin, DNA binding proteins, non-histone chromosomal proteins, coat proteins from DNA viruses, such as (XI 74, transcriptional factors also contain domains that bind DNA and therefore may be useful as nucleic aid condensing agents. Briefly, transcriptional factors such as C/CEBP, c-jun, c-fos, AP-1, AP-2, AP-3, CPF, Prot-1, Sp-1, Oct-1, Oct-2, CREP, and TFILD contain basic domains that bind DNA sequences. Organic polycationic agents include: spermine, spermidine, and purtrescine. The dimensions and of the physical properties of a polycationic agent can be extrapolated from the list above, to construct other polypeptide polycationic agents or to produce synthetic polycationic agents. Synthetic Polycationic Agents Synthetic polycationic agents which are useful include, for example, DEAE-dextran, polybrene. LipofectinD, and lipofectAMINED are monomers that form polycationic complexes when combined with polynucleotides or polypeptides. Immunodiagnostic Assays Neisserial antigens of the invention can be used in immunoassays to detect antibody levels (or, conversely, anti-Neisserial antibodies can be used to detect antigen levels). Immunoassays based on well defined, recombinant antigens can be developed to replace invasive diagnostics methods. Antibodies to Neisserial proteins within biological samples, including for example, blood or serum samples, can be detected. Design of the immunoassays is subject to a great deal of variation, and a variety of these are known in the art. Protocols for the immunoassay may be based, for example, upon competition, or direct reaction, or sandwich type assays. Protocols may also, for example, use solid supports, or may be by immunoprecipitation. Most assays involve the use of labeled antibody or polypeptide; the labels may be, for example, fluorescent, chemiluminescent, radioactive, or dye molecules. Assays which amplify the signals from the probe are also known; examples of which are assays which utilize biotin and avidin, and enzyme-labeled and mediated immunoassays, such as ELISA assays. Kits suitable for immunodiagnosis and containing the appropriate labeled reagents are constructed by packaging the appropriate materials, including the compositions of the invention, in suitable containers, along with the remaining reagents and materials (for example, suitable buffers, salt solutions, etc.) required for the conduct of the assay, as well as suitable set of assay instructions. Nucleic Acid Hybridisation "Hybridization" refers to the association of two nucleic acid sequences to one another by hydrogen bonding. Typically, one sequence will be fixed to a solid support and the other will be free in solution. Then, the two sequences will be placed in contact with one another under conditions that favor hydrogen bonding. Factors that affect this bonding include: the type and volume of solvent; reaction temperature; time of hybridization; agitation; agents to block the non-specific attachment of the liquid phase sequence to the solid support (Denhardt's reagent or BLOTTO); concentration of the sequences; use of compounds to increase the rate of association of sequences (dextran sulfate or polyethylene glycol); and the stringency of the washing conditions following hybridization. See Sambrook et al. [supra] Volume 2, chapter 9, pages 9.47 to 9.57. "Stringency" refers to conditions in a hybridization reaction that favor association of very similar sequences over sequences that differ. For example, the combination of temperature and salt concentration should be chosen that is approximately 120 to 200 DC below the calculated Tm of the hybrid under study. The temperature and salt conditions can often be determined empirically in preliminary experiments in which samples of genomic DNA immobilized on filters are hybridized to the sequence of interest and then washed under conditions of different stringencies. See Sambrook et al. at page 9.50. Variables to consider when performing, for example, a Southern blot are (1) the complexity of the DNA being blotted and (2) the homology between the probe and the sequences being detected. The total amount of the fragment(s) to be studied can vary a magnitude of 10, from 0.1 to 1 μg for a plasmid or phage digest to 10 "9 to 10 "8 g for a single copy gene in a highly complex eukaryotic genome. For lower complexity polynucleotides, substantially shorter blotting, hybridization, and exposure times, a smaller amount of starting polynucleotides, and lower specific activity of probes can be used. For example, a single-copy yeast gene can be detected with an exposure time of only 1 hour starting with 1 μg of yeast DNA, blotting for two hours, and hybridizing for 4-8 hours with a probe of 10 8 cpm/μg. For a single-copy mammalian gene a conservative approach would start with 10 μg of DNA, blot overnight, and hybridize overnight in the presence of 10% dextran sulfate using a probe of greater than 10 8 cpm/μg, resulting in an exposure time of -24 hours. Several factors can affect the melting temperature (Tm) of a DNA-DNA hybrid between the probe and the fragment of interest, and consequently, the appropriate conditions for hybridization and washing. In many cases the probe is not 100% homologous to the fragment. Other commonly encountered variables include the length and total G+C content of the hybridizing sequences and the ionic strength and formamide content of the hybridization buffer. The effects of all of these factors can be approximated by a single equation: Tm= 81 + 16.6(logi 0 Ci) + 0.4[%(G + C)]-0.6(%formamide) - 600/n-1.5(%mismatch). where Ci is the salt concentration (monovalent ions) and n is the length of the hybrid in base pairs (slightly modified from Meinkoth & Wahl (1984) Anal. Biochem. 138: 267-284). In designing a hybridization experiment, some factors affecting nucleic acid hybridization can be conveniently altered. The temperature of the hybridization and washes and the salt concentration during the washes are the simplest to adjust. As the temperature of the hybridization increases (ie. stringency), it becomes less likely for hybridization to occur between strands that are nonhomologous, and as a result, background decreases. If the radiolabeled probe is not completely homologous with the immobilized fragment (as is frequently the case in gene family and interspecies hybridization experiments), the hybridization temperature must be reduced, and background will increase. The temperature of the washes affects the intensity of the hybridizing band and the degree of background in a similar manner. The stringency of the washes is also increased with decreasing salt concentrations. In general, convenient hybridization temperatures in the presence of 50% formamide are 42 DC for a probe with is 95% to 100% homologous to the target fragment, 37 DC for 90% to 95%o homology, and 32DC for 85% to 90% homology. For lower homologies, formamide content should be lowered and temperature adjusted accordingly, using the equation above. If the homology between the probe and the target fragment are not known, the simplest approach is to start with both hybridization and wash conditions which are nonstringent. If non-specific bands or high background are observed after autoradiography, the filter can be washed at high stringency and reexposed. If the time required for exposure makes this approach impractical, several hybridization and/or washing stringencies should be tested in parallel. Nucleic Acid Probe Assays Methods such as PCR, branched DNA probe assays, or blotting techniques utilizing nucleic acid probes according to the invention can determine the presence of cDNA or mRNA. A probe is said to "hybridize" with a sequence of the invention if it can form a duplex or double stranded complex, which is stable enough to be detected. The nucleic acid probes will hybridize to the Neisserial nucleotide sequences of the invention (including both sense and antisense strands). Though many different nucleotide sequences will encode the amino acid sequence, the native Neisserial sequence is preferred because it is the actual sequence present in cells. mRNA represents a coding sequence and so a probe should be complementary to the coding sequence; single-stranded cDNA is complementary to mRNA, and so a cDNA probe should be complementary to the non-coding sequence. The probe sequence need not be identical to the Neisserial sequence (or its complement) — some variation in the sequence and length can lead to increased assay sensitivity if the nucleic acid probe can form a duplex with target nucleotides, which can be detected. Also, the nucleic acid probe can include additional nucleotides to stabilize the formed duplex. Additional Neisserial sequence may also be helpful as a label to detect the formed duplex. For example, a non-complementary nucleotide sequence may be attached to the 5' end of the probe, with the remainder of the probe sequence being complementary to a Neisserial sequence. Alternatively, non-complementary bases or longer sequences can be interspersed into the probe, provided that the probe sequence has sufficient complementarity with the a Neisserial sequence in order to hybridize therewith and thereby form a duplex which can be detected. The exact length and sequence of the probe will depend on the hybridization conditions, such as temperature, salt condition and the like. For example, for diagnostic applications, depending on the complexity of the analyte sequence, the nucleic acid probe typically contains at least 10-20 nucleotides, preferably 15-25, and more preferably at least 30 nucleotides, although it may be shorter than this. Short primers generally require cooler temperatures to form sufficiently stable hybrid complexes with the template. Probes may be produced by synthetic procedures, such as the triester method of Matteucci et al. [J. Am. Chem. Soc. (1981) 103:3185], or according to Urdea et al. [Proc. Natl. Acad. Sci. USA (1983) 80: 7461], or using commercially available automated oligonucleotide synthesizers. The chemical nature of the probe can be selected according to preference. For certain applications, DNA or RNA are appropriate. For other applications, modifications may be incorporated eg. backbone modifications, such as phosphorothioates or methylphosphonates, can be used to increase in vivo half-life, alter RNA affinity, increase nuclease resistance etc. [eg. see Agrawal & Iyer (1995) Curr Opin Biotechnol 6:12-19; Agrawal (1996) TIBTECH 14:376-387]; analogues such as peptide nucleic acids may also be used [eg. see Corey (1997) TIBTECH 15:224-229; Buchardt et al. (1993) TIBTECH 11:384-386]. One example of a nucleotide hybridization assay is described by Urdea et al. in international patent application WO92/02526 [see also US patent 5,124,246]. Alternatively, the polymerase chain reaction (PCR) is another well-known means for detecting small amounts of target nucleic acids. The assay is described in: Mullis et al [Meth. Enzymol. (1987) 155: 335-350]; US patent 4,683,195; and US patent 4,683,202. Two "primer" nucleotides hybridize with the target nucleic acids and are used to prime the reaction. The primers can comprise sequence that does not hybridize to the sequence of the amplification target (or its complement) to aid with duplex stability or, for example, to incorporate a convenient restriction site. Typically, such sequence will flank the desired Neisserial sequence. A thermostable polymerase creates copies of target nucleic acids from the primers using the original target nucleic acids as a template. After a threshold amount of target nucleic acids are generated by the polymerase, they can be detected by more traditional methods, such as Southern blots. When using the Southern blot method, the labelled probe will hybridize to the Neisserial sequence (or its complement). Also, mRNA or cDNA can be detected by traditional blotting techniques described in Sambrook et al [supra]. mRNA, or cDNA generated from mRNA using a polymerase enzyme, can be purified and separated using gel electrophoresis. The nucleic acids on the gel are then blotted onto a solid support, such as nitrocellulose. The solid support is exposed to a labelled probe and then washed to remove any unhybridized probe. Next, the duplexes containing the labeled probe are detected. Typically, the probe is labelled with a radioactive moiety. EXAMPLES The examples describe nucleic acid sequences which have been identified in N meningitidis, and N gonorrhoeae along with their respective and putative translation products. Not all of the nucleic acid sequences are complete ie. they encode less than the full- length wild-type protein. The examples are generally in the following format: • a nucleotide sequence which has been identified in N meningitidis • the putative translation product of said N meningitidis sequence • a computer analysis of said translation product based on database comparisons • a corresponding nucleotide sequence identified from N gonorrhoeae • the putative translation product of said N gonorrhoeae sequence • a comparision of the percentage of identity between the translation product of the N meningitidis sequence and the N gonorrhoeae sequence. • a corresponding nucleotide sequence identified from strain A of N. meningitidis • the putative translation product of said N. meningitidis strain A sequence • a comparision of the percentage of identity between the translation product of the N. meningitidis sequence and the N gonorrhoeae sequence. • a description of the characteristics of the protein which indicates that it might be suitably antigenic or immunogenic. Sequence comparisons were performed at ΝCBI (http://www.ncbi.nlm.nih.gov) using the algorithms BLAST, BLAST2, BLASTn, BLASTp, tBLASTn, BLASTx, & tBLASTx [eg. see also Altschul et al. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research 25:2289-3402]. Searches were performed against the following databases: non-redundant GenBank+EMBL+DDBJ+PDB sequences and non-redundant GenBank CDS translations+PDB+SwissProt+SPupdate+PLR sequences. Dots within nucleotide sequences represent nucleotides which have been arbitrarily introduced in order to maintain a reading frame. In the same way, double-underlined nucleotides were removed . Lower case letters represent ambiguities which arose during alignment of independent sequencing reactions (some of the nucleotide sequences in the examples are derived from combining the results of two or more experiments). Nucleotide sequences were scanned in all six reading frames to predict the presence of hydrophobic domains using an algorithm based on the statistical studies of Esposti et al. [Critical evaluation of the hydropathy of membrane proteins (1990) Eur J Biochem 190:207- 219]. These domains represent potential transmembrane regions or hydrophobic leader sequences. Open reading frames were predicted from fragmented nucleotide sequences using the program ORFFLNDER (NCBI). Underlined amino acid sequences indicate possible transmembrane domains or leader sequences in the ORFs, as predicted by the PSORT algorithm (http://www.psort.nibb.ac.jp). Functional domains were also predicted using the MOTIFS program (GCG Wisconsin & PROSITE). For each of the following examples: based on the presence of a putative leader sequence and/or several putative transmembrane domains (single-underlined) in the gonococcal protein, it is predicted that the proteins from N meningitidis and N. gonorrhoeae, and their respective epitopes, could be useful antigens or immunogenic compositions for vaccines or diagnostics. The standard techniques and procedures which may be employed in order to perform the invention (e.g. to utilize the disclosed sequences for vaccination or diagnostic purposes) were summarized above. This summary is not a limitation on the invention but, rather, gives examples that may be used, but are not required. In particular, the following methods were used to express, purify and biochemically characterize the proteins of the invention. Chromosomal DΝA Preparation N. meningitidis strain 2996 was grown to exponential phase in 100ml of GC medium, harvested by centrifugation, and resuspended in 5ml buffer (20%>(w/v) Sucrose, 50mM Tris- HC1, 50mM EDTA, pH8). After 10 minutes incubation on ice, the bacteria were lysed by adding 10ml of lysis solution (50mM ΝaCl, 1% Νa-Sarkosyl, 50μg/ml Proteinase K), and the suspension incubated at 37°C for 2 hours. Two phenol extractions (equilibrated to pH 8) and one CHCl 3 /isoamylalcohol (24:1) extraction were performed. DΝA was precipitated by addition of 0.3M sodium acetate and 2 volumes of ethanol, and collected by centrifugation. The pellet was washed once with 70%(v/v) ethanol and redissolved in 4.0ml TE buffer (lOmM Tris-HCl, ImM EDTA, pH 8.0). The DNA concentration was measured by reading the OD at 260 nm. Oligonucleotide design Synthetic oligonucleotide primers were designed on the basis of the coding sequence of each ORF, using (a) the meningococcus B sequence when available, or (b) the gonococcus/meningococcus A sequence, adapted to the codon preference usage of meningococcus as necessary. Any predicted signal peptides were omitted, by designing the 5' primers to sequence immediately downstream from the predicted leader sequence. For most ORFs, the 5' primers included two restriction enzyme recognition sites (BamHl-Ndel, BamHl-Nhel, EcoRl-Ndel or EcoRl-Nhel), depending on the restriction pattern of the gene of interest. The 3' primers included diXhol or a Hindϊϊl restriction site (table 1). This procedure was established in order to direct the cloning of each amplification product (corresponding to each ORF) into two different expression systems: pGEX-KG (using BamHl-Xhol, BamHl-Hindlll, EcoRl-Xhol or EcoRl-Hindlll), and pET21b+ (using Ndel- Xhol, Nhel-Xhol, Ndel-Hindϊll or Nhel-Hindlϊl). 5'-endprimertail: CGCGGATCCCATATG (BamHl-Ndel ) CGCGGATCCGCTAGC (BamHl-Nhel) CCGGAATTCTACATATG (EcoRl-Ndel) CCGGAATTCTAGCTAGC (EcoRl-Nhel) 3'-endprimertail: CCCGCTCGAG (Xhol) CCCGCTCGAG (HindllT) For cloning ORFs into the pGEX-His vector, the 5' and 3' primers contained only one restriction enzyme site (EcoRI, Kp ή l or Sail for the 5' primers and Pstl, Xbal, Sphl or Sail for the 3' primers). Again restriction sites were chosen according to the particular restriction pattern of the gene (table 1). 5 '-end primer tail: ( A ) AAAGAATTC (EcoRI ) ( AAA ) AAAGGTACC (Kpnϊ) 3 '-end primer tail: ( AAA ) AAACTGCAG (Pstl) (AAA) AAATCTAGA (Xbal) AAAGCATGC (Sphl) 5 ' or 3 '-end primer tail: AAAAAAGTCGAC (Sail) As well as containing the restriction enzyme recognition sequences, the primers included nucleotides which hybridized to the sequence to be amplified. The melting temperature depended on the number and type of hybridising nucleotides in the whole primer, and was determined for each primer using the formulae: T m = 4 (G+C)+ 2 (A+T) (tail excluded) T m = 64.9 + 0.41 (% GC) - 600/N (whole primer) The melting temperatures of the selected oligonucleotides were usually 65-70°C for the whole oligo and 50-55°C for the hybridising region alone. Table 1 shows the forward and reverse primers used for each amplification. In certain cases, the sequence of the primer does not exactly match the sequence of the predicted ORF. This is because when initial amplifications were performed, the complete 5' and/or 3' sequences for some meningococcal B ORFs were not known. However the corresponding sequences had been identified in Gonococcus or in Meningoccus A. Hence, when the Meningoccus B sequence was incomplete or uncertain, Gonococcal or Meningococcal A sequences were used as the basis for primer design. These sequences were altered to take account of codon preference. It can be appreciated that, once the complete sequence is identified, this approach will no longer be necessary. Oligonucleotides were synthesized using a Perkin Elmer 394 DNA/RNA Synthesizer, eluted from the columns in 2.0ml NH OH, and deprotected by 5 hours incubation at 56°C. The oligos were precipitated by addition of 0.3M Na- Acetate and 2 volumes ethanol. The samples were centrifuged and the pellets resuspended in either lOOμl or 1.0ml of water. The OD 260 was determined using a Perkin Elmer Lambda Bio spectophotometer and the concentration adjusted to 2-10pmol/μl. Amplification The standard PCR protocol was as follows: 50-200ng of genomic DNA was used as a template in the presence of 20-40μM of each oligonucletide primer, 400-800μM dNTPs solution, lx PCR buffer (including 1.5mM MgCl 2 ), 2.5 units TaqlONA polymerase (using Perkin-Elmer AmpliTaQ, GIBCO Platinum, Pwo DNA polymerase, or Tahara Shuzo Taq polymerase). In some cases, PCR was optimsed by the addition of lOμl DMSO or 50μl 2M Betaine. After a hot start (adding the polymerase during a preliminary 3 minute incubation of the whole mix at 95°C), each sample underwent a two-step amplification. The first 5 cycles were performed using the hybridization temperature that excluded the restriction enzyme tail of the primer (see above). This was followed by 30 cycles using the hybridization temperature calculated for the whole length oligos. The cycles were completed with a 10 minute extension step at 72°C. The standard cycles were as follows: Elongation times varied according to the length of the ORF to be amplified. Amplifications were performed using either a 9600 or a 2400 Perkin Elmer GeneAmp PCR System. To check the results, 1/10 of the amplification volume was loaded onto a 1-1.5% (w/v) agarose gel and the size of each amplified fragment compared with a DNA molecular weight marker. The amplified DNA was either loaded directly on a 1% agarose gel or first precipitated with ethanol and resuspended in a volume suitable to be loaded on a 1.0% agarose gel. The DNA fragment corresponding to the band of correct size was purified using the Qiagen Gel Extraction Kit, following the manufacturer's protocol. DNA fragments were eluted in a volume of 30μl or 50μl with either H2O or lOmM Tris, pH 8.5. Digestion of PCR fragments The purified DNA corresponding to the amplified fragment was doubly-digested with the appropriate restriction enzymes for; cloning into pET-21b+ and expressing the protein as a C-terminus His-tagged fusion, for cloning into pGEX-KG and expressing the protein as a N- terminus GST-fusion, and for cloning into pGEX-His and expressing the protein as a N-terminus GST-His tagged fusion. Each purified DNA fragment was incubated at 37°C for 3 hours to overnight with 20 units of appropriate restriction enzyme (New England Biolabs) in a volume of either 30 or 40μl in the presence of suitable digestion buffer. Digested fragments were purified using the QIAquick PCR purification kit (following the manufacturer's instructions) and eluted in a volume of 30μl or 50μl with either H2O or lOmM Tris, pH 8.5. The DNA concentration was determined by quantitative agarose gel electrophoresis (1.0% gel) in the presence of a titrated molecular weight marker. Digestion of the cloning vectors (pET22B, pGEX-KG, pTRC-His A, pET21b+, pGEX- KG, and pGEX-His) The vector pGEX-His is a modified pGEX-2T vector carrying a region encoding six histidine residues upstream of the thrombin cleavage site and containing the multiple cloning site of the vector pTRC99 (Pharmacia).10 μg plasmid was double-digested with 50 units of each restriction enzyme in 200 μl reaction volume in the presence of appropriate buffer by overnight incubation at 37°C. After loading the whole digestion on a 1%> agarose gel, the band corresponding to the digested vector was purified from the gel using the Qiagen QIAquick Gel Extraction Kit and the DNA was eluted in 50 μl of 10 mM Tris-HCl, pH 8.5. The DNA concentration was evaluated by measuring OD 260 of the sample, and adjusted to 50 μg/μl. 1 μl of plasmid was used for each cloning procedure. lOμg of plasmid vector was doubly-digested with 50 units of each restriction enzyme in a volume of 200μl with the appropriate buffer overnight at 37°C. The digest was loaded onto a 1.0% agarose gel and the band corresponding to the digested vector purified using the Qiagen QIAquick Gel Extraction Kit. DNA was eluted in 50μl of lOmM Tris-HCl, pH 8.5. The DNA concentration was evaluated by measuring OD 260nm and the concentration adjusted to 50μg/μl. lμl of plasmid was used for each cloning procedure. Cloning For some ORFs, the fragments corresponding to each ORF, previously digested and purified, were ligated in both pET22b and pGEX-KG. In a final volume of 20 μl, a molar ratio of 3:1 fragment/vector was ligated using 0.5 μl of NEB T4 DNA ligase (400 units/μl), in the presence of the buffer supplied by the manufacturer. The reaction was incubated at room temperature for 3 hours. In some experiments, ligation was performed using the Boheringer "Rapid Ligation Kit", following the manufacturer's instructions. In order to introduce the recombinant plasmid in a suitable strain, 100 μl E. coli DH5 competent cells were incubated with the ligase reaction solution for 40 minutes on ice, then at 37°C for 3 minutes, then, after adding 800 μl LB broth, again at 37°C for 20 minutes. The cells were then centrifuged at maximum speed in an Eppendorf microfuge and resuspended in approximately 200 μl of the supernatant. The suspension was then plated on LB ampicillin (lOO mg/ml ). The screening of the recombinant clones was performed by growing 5 randomly-chosen colonies overnight at 37 °C in either 2 ml (pGEX or pTC clones) or 5ml (pET clones) LB broth + 100 μg/ml ampicillin. The cells were then pelletted and the DNA extracted using the Qiagen QIAprep Spin Miniprep Kit, following the manufacturer's instructions, to a final volume of 30 μl. 5 μl of each individual miniprep (approximately lg ) were digested with either NdellXhol or BamHllXhol and the whole digestion loaded onto a 1 - 1.5%) agarose gel (depending on the expected insert size), in parallel with the molecular weight marker (1Kb DNA Ladder, GIBCO). The screening of the positive clones was made on the base of the correct insert size. For other ORFs, the fragments corresponding to each ORF, previously digested and purified, were ligated into both pET21b+ and pGEX-KG. A molar ratio of of 3:1 fragment/vector was used in a final volume of 20μl, that included 0.5μl T4 DNA ligase (400 units/μl, NEB) and ligation buffer supplied by the manufacturer. The reaction was performed at room temperature for 3 hours. In some experiments, ligation was performed using the Boheringer "Rapid Ligation Kit" and the manufacturer's protocol. Recombinant plasmid was transformed into lOOμl of competent E. coli DH5 or HB101 by incubating the ligase reaction solution and bacteria for 40 minutes on ice then at 37°C for 3 minutes. This was followed by the addition of 800μl LB broth and incubation at 37°C for 20 minutes. The cells were centrifuged at maximum speed in an Eppendorf microfuge, resuspended in approximately 200μl of the supernatant and plated onto LB ampicillin (lOOmg/ml ) agar. Screening for recombinant clones was performed by growing 5 randomly selected colonies overnight at 37°C in either 2.0ml (pGEX-KG clones) or 5.0ml (pET clones) LB broth + lOOμg/ml ampicillin. Cells were pelleted and plasmid DNA extracted using the Qiagen QIAprep Spin Miniprep Kit, following the manufacturer's instructions. Approximately lμg of each individual miniprep was digested with the appropriate restriction enzymes and the digest loaded onto a 1-1.5% agarose gel (depending on the expected insert size), in parallel with the molecular weight marker (lkb DNA Ladder, GIBCO). Positive clones were selected on the basis of the size of insert. ORFs were cloned into PGEX-His, by doubly-digesting the PCR product and ligating into similarly digested vector. After cloning, recombinant plasmids were transformed into the E.coli host W3110. Individual clones were grown overnight at 37°C in LB broth with 50μg/ml ampicillin. Certain ORFs may be cloned into the pGEX-HIS vector using EcoRl-Pstl cloning sites, or EcoRl-Sall, or Sall-Pstl. After cloning, the recombinant plasmids may be introduced in the E.coli host W3110. Expression Each ORF cloned into the expression vector may then be transformed into the strain suitable for expression of the recombinant protein product. 1 μl of each construct was used to transform 30 μl of E.coli BL21 (pGEX vector), E.coli TOP 10 (pTRC vector) or E.coli BL21- DE3 (pET vector), as described above. In the case of the pGEX-His vector, the same E.coli strain (W3110) was used for initial cloning and expression. Single recombinant colonies were inoculated into 2ml LB+Amp (100 μg/ml), incubated at 37°C overnight, then diluted 1:30 in 20 ml of LB+Amp (100 μg/ml) in 100 ml flasks, making sure that the OD 600 ranged between 0.1 and 0.15. The flasks were incubated at 30°C into gyratory water bath shakers until OD indicated exponential growth suitable for induction of expression (0.4-0.8 OD for pET and pTRC vectors; 0.8-1 OD for pGEX and pGEX-His vectors). For the pET, pTRC and pGEX- His vectors, the protein expression was induced by addiction of ImM LPTG, whereas in the case of pGEX system the final concentration of IPTG was 0.2 mM. After 3 hours incubation at 30°C, the final concentration of the sample was checked by OD. In order to check expression, 1ml of each sample was removed, centrifuged in a microfuge, the pellet resuspended in PBS, and analysed by 12% SDS-PAGE with Coomassie Blue staining. The whole sample was centrifuged at 6000 and the pellet resuspended in PBS for further use. GST-fusion proteins large-scale purification. For some ORFs, a single colony was grown overnight at 37°C on LB+Amp agar plate. The bacteria were inoculated into 20 ml of LB+Amp liquid colture in a water bath shaker and grown overnight. Bacteria were diluted 1 :30 into 600 ml of fresh medium and allowed to grow at the optimal temperature (20-37°C) to OD 550 0.8-1. Protein expression was induced with 0.2mM IPTG followed by three hours incubation. The culture was centrifuged at 8000 rpm at 4°C. The supernatant was discarded and the bacterial pellet was resuspended in 7.5 ml cold PBS. The cells were disrupted by sonication on ice for 30 sec at 40W using a Branson sonifier B-15, frozen and thawed two times and centrifuged again. The supernatant was collected and mixed with 150μl Glutatione-Sepharose 4B resin (Pharmacia) (previously washed with PBS) and incubated at room temperature for 30 minutes. The sample was centrifuged at 700g for 5 minutes at 4C. The resin was washed twice with 10 ml cold PBS for 10 minutes, resuspended in 1ml cold PBS, and loaded on a disposable column. The resin was washed twice with 2ml cold PBS until the flow-through reached OD 280 of 0.02-0.06. The GST-fusion protein was eluted by addition of 700μl cold Glutathione elution buffer lOmM reduced glutathione, 50mM Tris-HCl) and fractions collected until the OD 280 was 0.1. 21 μl of each fraction were loaded on a 12% SDS gel using either Biorad SDS-PAGE Molecular weight standard broad range (Ml) (200, 116.25, 97.4, 66.2, 45, 31, 21.5, 14.4, 6.5 kDa) or Amersham Rainbow Marker (M") (220, 66, 46, 30, 21.5, 14.3 kDa) as standards. As the MW of GST is 26kDa, this value must be added to the MW of each GST-fusion protein. For other ORFs, for each clone to be purified as a GST-fusion, a single colony was streaked out and grown overnight at 37°C on a LB/Amp. (lOOμg/ml) agar plate. An isolated colony from this plate was inoculated into 20ml of LB/Amp (100 μg/ml) liquid medium and grown overnight at 37°C with shaking. The overnight culture was diluted 1 :30 into 600ml LB/Amp (lOOμg/ml) liquid medium and allowed to grow at the optimal temperature (20- 37°C) until the OD 55 on m reached 0.6-0.8. Recombinant protein expression was induced by addition of IPTG (final concentration 0.2mM) and the culture incubated for a further 3 hours. Bacteria were harvested by centrifugation at 8000xg for 15 min at 4°C. The bacterial pellet was resuspended in 7.5ml cold PBS. Cells were disrupted by sonication on ice four times for 30 sec at 40W using a Branson sonifier 450 and centrifuged at 13 OOOxg for 30 min at 4°C. The supernatant was collected and mixed with 150μl Glutatione- Sepharose 4B resin (Pharmacia), previously equilibrated with PBS, and incubated at room temperature with gentle agitation for 30 min. The batch-wise preparation was centrifuged at 700x for 5 min at 4°C and the supernatant discarded. The resin was washed twice (batchwise) with 10ml cold PBS for 10 min, resuspended in 1ml cold PBS, and loaded onto a disposable column. The resin continued to be washed with cold PBS, until the OD 280nm of the flow-through reached 0.02-0.01. The GST-fusion protein was eluted by addition of 700μl cold glutathione elution buffer (lOmM reduced glutathione, 50mM Tris-HCl pH 8.0) and fractions collected, until the OD 2 8o nm of the eluate indicated all the recombinant protein was obtained. 20μl aliquots of each elution fraction were analyzed by SDS-PAGE using a 12%> gel. The molecular mass of the purified proteins was determined using either the Bio-Rad broad range molecular weight standard (Ml) (200, 116, 97.4, 66.2, 45.0, 31.0, 21.5, 14.4, 6.5 kDa) or the Amersham Rainbow Marker (M2) (220, 66.2, 46.0, 30.0, 21.5, 14.3 kDa). The molecular weights of GST-fusion proteins are a combination of the 26 kDa GST protein and its fusion partner. Protein concentrations were estimated using the Bradford assay. His-fusion soluble proteins large-scale purification. For some ORFs, a single colony was grown overnight at 37°C on a LB + Amp agar plate. The bacteria were inoculated into 20ml of LB+Amp liquid culture and incubated overnight in a water bath shaker. Bacteria were diluted 1 :30 into 600ml fresh medium and allowed to grow at the optimal temperature (20-37°C) to OD 550 0.6-0.8. Protein expression was induced by addition of 1 mM IPTG and the culture further incubated for three hours. The culture was centrifuged at 8000 rpm at 4°C, the supernatant was discarded and the bacterial pellet was resuspended in 7.5ml cold lOmM imidazole buffer (300 mM NaCl, 50 mM phosphate buffer, 10 mM imidazole, pH 8). The cells were disrupted by sonication on ice for 30 sec at 40W using a Branson sonifier B-15, frozen and thawed two times and centrifuged again. The supernatant was collected and mixed with 150μl Ni 2+ -resin (Pharmacia) (previously washed with lOmM imidazole buffer) and incubated at room temperature with gentle agitation for 30 minutes. The sample was centrifuged at lOOg for 5 minutes at 4°C. The resin was washed twice with 10 ml cold lOmM imidazole buffer for 10 minutes, resuspended in 1ml cold lOmM imidazole buffer and loaded on a disposable column. The resin was washed at 4°C with 2ml cold lOmM imidazole buffer until the flow-through reached the O.D 280 of 0.02-0.06. The resin was washed with 2ml cold 20mM imidazole buffer (300 mM NaCl, 50 mM phosphate buffer, 20 mM imidazole, pH 8) until the flow- through reached the O.D 280 of 0.02-0.06. The His-fusion protein was eluted by addition of 700μl cold 250mM imidazole buffer (300 mM NaCl, 50 mM phosphate buffer, 250 mM imidazole, pH 8) and fractions collected until the O.D 2 0 was 0.1. 2 lμl of each fraction were loaded on a 12% SDS gel. His-fusion insoluble proteins large-scale purification. A single colony was grown overnight at 37 °C on a LB + Amp agar plate. The bacteria were inoculated into 20 ml of LB+Amp liquid culture in a water bath shaker and grown overnight. Bacteria were diluted 1 :30 into 600ml fresh medium and let to grow at the optimal temperature (37°C) to O.D550 0.6-0.8. Protein expression was induced by addition of 1 mM IPTG and the culture further incubated for three hours. The culture was centrifuged at 8000φm at 4°C. The supernatant was discarded and the bacterial pellet was resuspended in 7.5 ml buffer B (urea 8M, lOmM Tris-HCl, lOOmM phosphate buffer, pH 8.8). The cells were disrupted by sonication on ice for 30 sec at 40W using a Branson sonifier B-15, frozen and thawed twice and centrifuged again. The supernatant was stored at -20°C, while the pellets were resuspended in 2 ml guanidine buffer (6M guanidine hydrochloride, lOOmM phosphate buffer, 10 mM Tris-HCl, pH 7.5) and treated in a homogenizer for 10 cycles. The product was centrifuged at 13000 φm for 40 minutes. The supernatant was mixed with 150μl Ni 2+ -resin (Pharmacia) (previously washed with buffer B) and incubated at room temperature with gentle agitation for 30 minutes. The sample was centrifuged at 700 g for 5 minutes at 4°C. The resin was washed twice with 10 ml buffer B for 10 minutes, resuspended in 1ml buffer B, and loaded on a disposable column. The resin was washed at room temperature with 2ml buffer B until the flow-through reached the OD 28 o of 0.02-0.06. The resin was washed with 2ml buffer C (urea 8M, lOmM Tris-HCl, lOOmM phosphate buffer, pH 6.3) until the flow-through reached the O.D 80 of 0.02-0.06. The His-fusion protein was eluted by addition of 700μl elution buffer (urea 8M, lOmM Tris-HCl, lOOmM phosphate buffer, pH 4.5) and fractions collected until the OD 280 was 0.1. 2 lμl of each fraction were loaded on a 12% SDS gel. Purification of His-fusion proteins. For each clone to be purified as a His-fusion, a single colony was streaked out and grown overnight at 37°C on a LB/Amp (100 μg/ml) agar plate. An isolated colony from this plate was inoculated into 20ml of LB/Amp (100 μg/ml) liquid medium and grown overnight at 37°C with shaking. The overnight culture was diluted 1 :30 into 600ml LB/Amp (100 μg/ml) liquid medium and allowed to grow at the optimal temperature (20-37°C) until the OD 550nm reached 0.6-0.8. Expression of recombinant protein was induced by addition of IPTG (final concentration 1.OmM) and the culture incubated for a further 3 hours. Bacteria were harvested by centrifugation at 8000xg for 15 min at 4°C. The bacterial pellet was resuspended in 7.5ml of either (i) cold buffer A (300mM NaCl, 50mM phosphate buffer, lOmM imidazole, pH 8.0) for soluble proteins or (ii) buffer B (8M urea, lOmM Tris-HCl, lOOmM phosphate buffer, pH 8.8) for insoluble proteins. Cells were disrupted by sonication on ice four times for 30 sec at 40W using a Branson sonifier 450 and centrifuged at 13 OOOxg for 30 min at 4°C. For insoluble proteins, pellets were resuspended in 2.0 ml buffer C (6M guanidine hydrochloride, lOOmM phosphate buffer, lOmM Tris-HCl, pH 7.5) and treated with a Dounce homogenizer for 10 cycles. The homogenate was centrifuged at 13 OOOxg for 40 min and the supernatant retained. Supernatants for both soluble and insoluble preparations were mixed with 150μl Ni 2+ - resin (previously equilibrated with either buffer A or buffer B, as appropriate) and incubated at room temperature with gentle agitation for 30 min. The resin was Chelating Sepharose Fast Flow (Pharmacia), prepared according to manufacturers protocol. The batch-wise preparation was centrifuged at 700xg for 5 min at 4°C and the supernatant discarded. The resin was washed twice (batch- wise) with 10ml buffer A or B for 10 min, resuspended in 1.0 ml buffer A or B and loaded onto a disposable column. The resin continued to be washed with either (i) buffer A at 4°C or (ii) buffer B at room temperature, until the OD 28 o nm of the flow-through reached 0.02-0.01. The resin was further washed with either (i) cold buffer C (300mM NaCl, 50mM phosphate buffer, 20mM imidazole, pH 8.0) or (ii) buffer D (8M urea, lOmM Tris- HCl, lOOmM phosphate buffer, pH 6.3) until the the OD 280nm of the flow-through reached 0.02-0.01. The His-fusion protein was eluted by addition of 700μl of either (i) cold elution buffer A (300mM NaCl, 50mM phosphate buffer, 250mM imidazole, pH 8.0) or (ii) elution buffer B (8 M urea, lOmM Tris-HCl, lOOmM phosphate buffer, pH 4.5) and fractions collected until the O.D 28 o nm indicated all the recombinant protein was obtained. 20μl aliquots of each elution fraction were analyzed by SDS-PAGE using a 12%> gel. Protein concentrations were estimated using the Bradford assay. His-fusion proteins renaturation In the cases where denaturation was required to solubilize proteins, a renaturation step was employed prior to immunization. Glycerol was added to the denatured fractions obtained above to give a final concentration of 10%>(v/v). The proteins were diluted to 200μg/ml using dialysis buffer I (10%> (v/v) glycerol, 0.5M arginine, 50mM phosphate buffer, 5.0mM reduced glutathione, 0.5mM oxidised glutathione, 2.0M urea, pH 8.8) and dialysed against the same buffer for 12-14 hours at 4°C. Further dialysis was performed with buffer II (10% > (v/v) glycerol, 0.5M arginine, 50mM phosphate buffer, 5.0mM reduced glutathione, 0.5mM oxidised glutathione, pH 8.8) for 12-14 hours at 4°C. Alternatively, 10%> glycerol was added to the denatured proteins. The proteins were then diluted to 20μg/ml using dialysis buffer I (10%> glycerol, 0.5M arginine, 50mM phosphate buffer, 5mM reduced glutathione, 0.5mM oxidised glutathione, 2M urea, pH 8.8) and dialysed against the same buffer at 4°C for 12-14 hours. The protein was further dialysed against dialysis buffer II (10%> glycerol, 0.5M arginine, 50mM phosphate buffer, 5mM reduced glutathione, 0.5mM oxidised glutathione, pH 8.8) for 12-14 hours at 4°C. Protein concentration was evaluated using the formula: Protein (mg/ml) = (1.55 x OD 280 ) - (0.76 x OD 260 ) Purification of proteins To analyse the solubility, pellets obtained from 3.0ml cultures were resuspended in 500μl buffer Ml (PBS pH 7.2). 25μl of lysozyme (lOmg/ml) was added and the bacteria incubated for 15 min at 4°C. Cells were disrupted by sonication on ice four times for 30 sec at 40W using a Branson sonifier 450 and centrifuged at 13 OOOxg for 30 min at 4°C. The supernatant was collected and the pellet resuspended in buffer M2 [8M urea, 0.5M NaCl, 20mM imidazole and 0.1M NaH 2 PO 4 ] and incubated for 3 to 4 hours at 4°C. After centrifugation, the supernatant was collected and the pellet resuspended in buffer M3 [6M guanidinium-HCl, 0.5M NaCl, 20mM imidazole and 0.1M NaH 2 PO 4 ] overnight at 4°C. The supernatants from all steps were analysed by SDS-PAGE. Some proteins were found to be soluble in PBS, others need urea or guanidium-HCl for solubilization. For preparative scale purifications, 500ml cultures were induced and fusion proteins solubilized in either buffer Ml, M2 or M3 using the procedure described above. Crude extracts were loaded onto a Ni-NTA superflow column (Quiagen) equilibrated with buffer Ml, M2 or M3 depending on the solubilization buffer employed. Unbound material was eluted by washing the column with the same buffer. The recombinant fusion protein was eluted with the corresponding buffer containing 500mM imidazole then dialysed against the same buffer in the absence of imidazole. Mice immunisations 20μg of each purified protein are used to immunise mice intraperitoneally. In the case of some ORFs, Balb-C mice were immunised with Al(OH) 3 as adjuvant on days 1, 21 and 42, and immune response was monitored in samples taken on day 56. For other ORFs, CDl mice could be immunised using the same protocol. For ORFs 25 and 40, CDl mice were immunised using Freund's adjuvant, and the same immunisation protocol was used, except that the immune response was measured on day 42, rather than 56. Similarly, for still other ORFs, CDl mice were immunised with Freund's adjuvant, but the immune response was measured on day 49. Alternatively, 20μg of each purified protein was mixed with Freund's adjuvant and used to immunise CDl mice intraperitoneally. For many of the proteins, the immunization was performed on days 1, 21 and 35, and immune response was monitored in samples taken on days 34 and 49. For some proteins, the third immunization was performed on day 28, rather than 35, and the immune response was measured on days 20 and 42, rather than 34 and 49. ELISA assay (sera analysis) The acapsulated MenB M7 strain was plated on chocolate agar plates and incubated overnight at 37°C. Bacterial colonies were collected from the agar plates using a sterile dracon swab and inoculated into 7ml of Mueller-Hinton Broth (Difco) containing 0.25%> Glucose. Bacterial growth was monitored every 30 minutes by following OD 620 . The bacteria were let to grow until the OD reached the value of 0.3-0.4. The culture was centrifuged for 10 minutes at 10000 φm. The supernatant was discarded and bacteria were washed once with PBS, resuspended in PBS containing 0.025% formaldehyde, and incubated for 2 hours at room temperature and then overnight at 4°C with stirring. lOOμl bacterial cells were added to each well of a 96 well Greiner plate and incubated overnight at 4°C. The wells were then washed three times with PBT washing buffer (0.1 %> Tween-20 in PBS). 200 μl of saturation buffer (2.7% Polyvinylpyrrolidone 10 in water) was added to each well and the plates incubated for 2 hours at 37°C. Wells were washed three times with PBT. 200 μl of diluted sera (Dilution buffer: 1% BSA, 0.1% Tween-20, 0.1% NaN 3 in PBS) were added to each well and the plates incubated for 90 minutes at 37°C. Wells were washed three times with PBT. 100 μl of HRP-conjugated rabbit anti-mouse (Dako) serum diluted 1 :2000 in dilution buffer were added to each well and the plates were incubated for 90 minutes at 37°C. Wells were washed three times with PBT buffer. 100 μl of substrate buffer for HRP (25 ml of citrate buffer pH5, 10 mg of O-phenildiamine and 10 μl of H O) were added to each well and the plates were left at room temperature for 20 minutes. 100 μl H 2 SO 4 was added to each well and OD 49 o was followed. The ELISA was considered positive when OD490 was 2.5 times the respective pre-immune sera. Alternatively, The acapsulated MenB M7 strain was plated on chocolate agar plates and incubated overnight at 37°C. Bacterial colonies were collected from the agar plates using a sterile dracon swab and inoculated into Mueller-Hinton Broth (Difco) containing 0.25% > Glucose. Bacterial growth was monitored every 30 minutes by following OD 62 o- The bacteria were let to grow until the OD reached the value of 0.3-0.4. The culture was centrifuged for 10 minutes at 10 OOOφrn. The supernatant was discarded and bacteria were washed once with PBS, resuspended in PBS containing 0.025%> formaldehyde, and incubated for 1 hour at 37°C and then overnight at 4°C with stirring. lOOμl bacterial cells were added to each well of a 96 well Greiner plate and incubated overnight at 4°C. The wells were then washed three times with PBT washing buffer (0.1% Tween-20 in PBS). 200μl of saturation buffer (2.7% Polyvinylpyrrolidone 10 in water) was added to each well and the plates incubated for 2 hours at 37°C. Wells were washed three times with PBT. 200μl of diluted sera (Dilution buffer: 1% BSA, 0.1%) Tween-20, 0.1% NaN 3 in PBS) were added to each well and the plates incubated for 2 hours at 37°C. Wells were washed three times with PBT. lOOμl of HRP-conjugated rabbit anti-mouse (Dako) serum diluted 1 :2000 in dilution buffer were added to each well and the plates were incubated for 90 minutes at 37°C. Wells were washed three times with PBT buffer. lOOμl of substrate buffer for HRP (25ml of citrate buffer pH5, lOmg of O- phenildiamine and lOμl of H 2 O 2 ) were added to each well and the plates were left at room temperature for 20 minutes. lOOμl of 12.5%o H 2 SO 4 was added to each well and OD 4 0 was followed. The ELISA titers were calculated abitrarely as the dilution of sera which gave an OD 49 o value of 0.4 above the level of preimmune sera. The ELISA was considered positive when the dilution of sera with OD 490 of 0.4 was higher than 1 :400. FACScan bacteria Binding Assay procedure. The acapsulated MenB M7 strain was plated on chocolate agar plates and incubated overnight at 37°C. Bacterial colonies were collected from the agar plates using a sterile dracon swab and inoculated into 4 tubes containing 8ml each Mueller-Hinton Broth (Difco) containing 0.25%> glucose. Bacterial growth was monitored every 30 minutes by following OD 62 o- The bacteria were let to grow until the OD reached the value of 0.35-0.5. The culture was centrifuged for 10 minutes at 4000φm. The supernatant was discarded and the pellet was resuspended in blocking buffer (1%> BSA in PBS, 0.4%, NaN 3 ) and centrifuged for 5 minutes at 4000φm. Cells were resuspended in blocking buffer to reach OD 620 of 0.07. lOOμl bacterial cells were added to each well of a Costar 96 well plate. lOOμl of diluted (1 :100, 1:200, 1 :400) sera (in blocking buffer) were added to each well and plates incubated for 2 hours at 4°C. Cells were centrifuged for 5 minutes at 4000φm, the supernatant aspirated and cells washed by addition of 200μl/well of blocking buffer in each well. lOOμl of R-Phicoerytrin conjugated F(ab) 2 goat anti -mouse, diluted 1 :100, was added to each well and plates incubated for 1 hour at 4°C. Cells were spun down by centrifugation at 4000φm for 5 minutes and washed by addition of 200μl/well of blocking buffer. The supernatant was aspirated and cells resuspended in 200μl/well of PBS, 0.25%> formaldehyde. Samples were transferred to FACScan tubes and read. The condition for FACScan (Laser Power 15mW) setting were: FL2 on; FSC-H threshold:92; FSC PMT Voltage: E 01; SSC PMT: 474; Amp. Gains 6.1; FL-2 PMT: 586; compensation values: 0. OMV preparations Bacteria were grown overnight on 5 GC plates, harvested with a loop and resuspended in 10 ml 20mM Tris-HCl. Heat inactivation was performed at 56°C for 30 minutes and the bacteria disrupted by sonication for 10' on ice ( 50%> duty cycle, 50% output ). Unbroken cells were removed by centrifugation at 5000g for 10 minutes and the total cell envelope fraction recovered by centrifugation at 50000g at 4°C for 75 minutes. To extract cytoplasmic membrane proteins from the crude outer membranes, the whole fraction was resuspended in 2% sarkosyl (Sigma) and incubated at room temperature for 20 minutes. The suspension was centrifuged at 10000g for 10 minutes to remove aggregates, and the supernatant further ultracentrifuged at 50000 for 75 minutes to pellet the outer membranes. The outer membranes were resuspended in lOmM Tris-HCl, pH8 and the protein concentration measured by the Bio-Rad Protein assay, using BSA as a standard. Whole Extracts preparation Bacteria were grown overnight on a GC plate, harvested with a loop and resuspended in 1ml of 20mM Tris-HCl. Heat inactivation was performed at 56°C for 30' minutes. Western blotting Purified proteins (500ng/lane), outer membrane vesicles (5μg) and total cell extracts (25μg) derived from MenB strain 2996 were loaded onto a 12% SDS-polyacrylamide gel and transferred to a nitrocellulose membrane. The transfer was performed for 2 hours at 150mA at 4°C, using transfer buffer (0.3 % Tris base, 1.44 % glycine, 20% (v/v) methanol). The membrane was saturated by overnight incubation at 4°C in saturation buffer (10%> skimmed milk, 0.1%) Triton XI 00 in PBS). The membrane was washed twice with washing buffer (3% skimmed milk, 0.1 % Triton XI 00 in PBS) and incubated for 2 hours at 37°C with mice sera diluted 1 :200 in washing buffer. The membrane was washed twice and incubated for 90 minutes with a 1:2000 dilution of horseradish peroxidase labelled anti-mouse Ig. The membrane was washed twice with 0.1% Triton X100 in PBS and developed with the Opti- 4CN Substrate Kit (Bio-Rad). The reaction was stopped by adding water. Bactericidal assay MC58 and 2996 strains were grown overnight at 37°C on chocolate agar plates. 5-7 colonies were collected and used to inoculate 7ml Mueller-Hinton broth. The suspension was incubated at 37°C on a nutator and let to grow until OD 6 0 was in between 0.5-0.8. The culture was aliquoted into sterile 1.5ml Eppendorf tubes and centrifuged for 20 minutes at maximum speed in a microfuge. The pellet was washed once in Gey's buffer (Gibco) and resuspended in the same buffer to an OD 620 of 0.5, diluted 1 :20000 in Gey's buffer and stored at 25°C. 50μl of Gey's buffer/l%o BSA was added to each well of a 96-well tissue culture plate. 25μl of diluted (1 :100) mice sera (dilution buffer: Gey's buffer/0.2% BSA) were added to each well and the plate incubated at 4°C. 25μl of the previously described bacterial suspension were added to each well. 25μl of either heat-inactivated (56°C waterbath for 30 minutes) or normal baby rabbit complement were added to each well. Immediately after the addition of the baby rabbit complement, 22μl of each sample/well were plated on Mueller- Hinton agar plates (time 0). The 96-well plate was incubated for 1 hour at 37°C with rotation and then 22μl of each sample/well were plated on Mueller-Hinton agar plates (time 1). After overnight incubation the colonies corresponding to time 0 and time lh were counted. Gene Variability The ORF 4 and 919 genes were amplified by PCR on chromosomal DNA extracted from various Neisseria strains (see list of strains). The following oligonucleotides used as PCR primers were designed in the upstream and downstream regions of the genes: orf4.1 (forward) CGAATCCGGACGGCAGGACTC orf4.3 (reverse) GGCAGGGAATGGCGGATTAAAG 919.1 (forward) AAAATGCCTCTCCACGGCTG or CTGCGCCCTGTGTTAAAATCCCCT 919.6 (reverse) CAAATAAGAAAGGAATTTTG or GGTATCGCAAAACTTCGCCTTAATGCG The PCR cycling conditions were: 1 cycle 2 min. at 94° 30 cycles 30 sec. at 94° 30 sec. at ~ 54° or ~ 60° ( in according to Tm of the primers) 40 sec. at 72° 1 cycle 7 min. at 72 > c The PCR products were purified from 1 % agarose gel and sequenced using the following primers: orf4.1 (forward) CGAATCCGGACGGCAGGACTC orf4.2 (forward) CGACCGCGCCTTTGGGACTG orf4.3 (reverse) GGCAGGGAATGGCGGATTAAAG orf4.4 (reverse) TCTTTGAGTTTGATCCAACC 919.1 (forward) AAAATGCCTCTCCACGGCTG or CTGCGCCCTGTGTTAAAATCCCCT 919.2 (forward) ATCCTTCCGCCTCGGCTGCG 919.3 (forward) AAAACAGCGGCACAATCGAC 919.4 (forward) ATAAGGGCTACCTCAAACTC 919.5 (forward) GCGCGTGGATTATTTTTGGG 919.6 (reverse) CAAATAAGAAAGGAATTTTG or GGTATCGCAAAACTTCGCCTTAATGCG 919.7 (reverse) CCCAAGGTAATGTAGTGCCG 919.8 (reverse) TAAAAAAAAGTTCGACAGGG 919.9 (reverse) CCGTCCGCCTGTCGTCGCCC 919.10 (reverse) TCGTTCCGGCGGGGTCGGGG All documents cited herein are incoφorated by reference in their entireties. The following Examples are presented to illustrate, not limit, the invention EXAMPLE 1 Using the above-described procedures, the following oligonucleotide primers were employed in the polymerase chain reaction (PCR) assay in order to clone the ORFs as indicated: Table 1: Oligonucleotides used for PCR for Examples 2-10 Localization of the ORFs The following DNA and amino acid sequences are identified by titles of the following form: [g, m, or a] [#].[seq or pep], where "g" means a sequence from N gonorrhoeae, "m" means a sequence from N. meningitidis B, and "a" means a sequence from N meningitidis A; "#" means the number of the sequence; "seq" means a DΝA sequence, and "pep" means an amino acid sequence. For example, "gOOl.seq" refers to an N gonorrohoeae DΝA sequence, number 1. The presence of the suffix "-1" to these sequences indicates an additional sequence found for the same ORF, thus, data for an ORF having both an unsuffixed and a suffixed sequence designation applies to both such designated sequences. Further, open reading frames are identified as ORF #, where "#" means the number of the ORF, corresponding to the number of the sequence which encodes the ORF, and the ORF designations may be suffixed with ".ng" or ".a", indicating that the ORF corresponds to a N. gonorrhoeae sequence or a N meningitidis A sequence, respectively. The word "partial" before a sequence indicates that the sequence may be a partial or a complete ORF. Computer analysis was performed for the comparisons that follow between "g", "m", and "a" peptide sequences; and therein the "pep" suffix is implied where not expressly stated. Further, in the event of a conflict between the text immediately preceding and describing which sequences are being compared, and the designated sequences being compared, the designated sequence controls and is the actual sequence being compared. ORF: contig: 279 gnm4.seq The following partial DNA sequence was identified in N meningitidis <SEQ ID 3039>: m279.seq 1 ATAACGCGGA TTTGCGGCTG CTTGATTTCA ACGGTTTTCA GGGCTTCGGC 51 AAGTTTGTCG GCGGCGGGTT TCATCAGGCT GCAATGGGAA GGTACGGACA 101 CGGGCAGCGG CAGGGCGCGT TTGGCACCGG CTTCTTTGGC GGCAGCCATG 151 GCGCGTCCGA CGGCGGCGGC GTTGCCTGCA ATCACGATTT GTCCGGGTGA 201 GTTGAAGTTG ACGGCTTCGA CCACTTCGCT TTGGGCGGCT TCGGCACAAA 251 TGGCTTTAAC CTGCTCATCT TCCAAGCCGA GAATCGCCGC CATTGCGCCC 301 ACGCCTTGCG GTACGGCGGA CTGCATCAGT TCGGCGCGCA GGCGCACGAG 351 TTTGACCGCG TCGGCAAAAT TCAATGCGCC GGCGGCAACG AGTGCGGTGT 401 ATTCGCCGAG GCTGTGTCCG GCAACGGCGG CAGGCGTTTT GCCGCCCGCT 451 TCTAAATAG This corresponds to the amino acid sequence <SEQ ID 3040; ORF 279>: m279.ρep 1 ITRICGCLIS TVFRASASLS AAGFIRLQWE GTDTGSGRAR LAPASLAAAM 51 ARPTAAALPA ITICPGELKL TASTTSLWAA SAQMALTCSS SKPRIAAIAP 101 TPCGTADCIS SARRRTSLTA SAKFΝAPAAT SAVYSPRLCP ATAAGVLPPA 151 SK* The following partial DΝA sequence was identified in N. gonorrhoeae <SEQ ID 3041>: g279.seq 1 atgacgcgga tttgcggctg cttgatttca acggttttga gtgtttcggc 51 aagtttgtcg gcggcgggtt tcatcaggct gcaatgggaa ggaacggata 101 ccggcagcgg cagggcgcgt ttggctccgg cttctttggc ggcagccatg 151 gtgcgtccga cggcggcggc gttgcctgca atcacgactt gtccgggcga 201 gttgaagttg acggcttcga ccacttcgcc ctgtgcggat tcggcacaaa 251 tctgcctgac ctgttcatct tccaaaccca aaatggccgc cattgcgcct 301 acgccttgcg gtacggcgga ctgcatcagt tcggcgcgca ggcggacgag 351 tttgacggca tcggcaaaat ccaatgcttc ggcggcgaca agcgcggtgt 401 attcgccgag gctgtgtccg gcaacggcgg caggcgtttt gccgcccact 451 tccaaatag This corresponds to the amino acid sequence <SEQ ID 3042; ORF 279.ng>: g 79.pep 1 MTRICGCLIS TVLSVSASLS AAGFIRLQWE GTDTGSGRAR LAPASLAAAM 51 VRPTAAALPA ITTCPGELKL TASTTSPCAD SAQICLTCSS SKPKMAAIAP 101 TPCGTADCIS SARRRTSLTA SAKSΝASAAT SAVYSPRLCP ATAAGVLPPT 151 SK* ORF 279 shows 89.5% identity over a 152 aa overlap with a predicted ORF (ORF 279.ng) from N. gonorrhoeae: 10 20 30 40 50 60 m279. pep ITRICGCLISTVFRASASLSAAGFIRLQWEGTDTGSGRARLAPASLAAAMARPTAAALPA =11111111111= =11111111111111111111111111111111111=111111111 g279 MTRICGCLISTVLSVSASLSAAGFIRLQWEGTDTGSGRARLAPASLAAAMVRPTAAALPA 10 20 30 40 50 60 70 80 90 100 110 120 m279.pep ITICPGELKLTASTTSLWAASAQMALTCSSSKPRIAAIAPTPCGTADCISSARRRTSLTA I I 1 1 1 1 1 1 1 i f 1 1 1 J I 1 1 1 = l l l l l l l l = = I M I I M I I M I I I I M I I I M I M g279 ITTCPGELKLTASTTSPCADSAQICLTCSSSKPKMAAIAPTPCGTADCISSARRRTSLTA 70 80 90 100 110 120 130 140 150 m279. pep SAKFNAPAATSAVYSPRLCPATAAGVLPPASKX III II g279 SAKSNASAATSAVYSPRLCPATAAGVLPPTSKX 130 140 150 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 3043>: a279.seq 1 A ATTGGAACCNΝCCNΝGGAA TTTGCGGCTG CTTGATTTCA ACGGTTTΝΝA GGGCTTCGGC 51 GAGTTTGTCG GCGGCGGGTT TCATGAGGCT GCAATGGGAA GGTACΝGACA 101 CNGGCAGCGG CAGGGCGCGT TTGGCGCCGG CTTCTTTGGC GGCAAGCATA 151 GCGCGCTCGA CGGCGGCGGC ATTGCCTGCA ATCACGACTT GTCCGGGCGA 201 GTTGAAGTTG ACGGCTTCAA CCACTTCATC CTGTGCGGAT TCGGCGCAAA 251 TTTGTTTTAC CTGTTCATCT TCCAAGCCGA GAATCGCCGC CATTGCGCCC 301 ACGCCTTGCG GTACGGCGGA CTGCATCAGT TCGGCGCGCA ΝGCGCACGAG 351 TTTGACCGCG TCGGCAAAAT CCAATGCGCC GGCGGCAACΝ AGTGCGGTGT 401 ATTCGCCGAN GCTGTGTCCG GCAACGGCGG CAGGCGTTTT GCCGCCCGCT 451 TCCGAATAG This corresponds to the amino acid sequence <SEQ ID 3044; ORF 279.a>: a279.pep 1 MTXICGCLIS TVXRASASLS AAGFMRLQWE GTDTGSGRAR LAPASLAASI 51 ARSTAAALPA ITTCPGELKL TASTTSSCAD SAQICFTCSS SKPRIAAIAP 101 TPCGTADCIS SARXRTSLTA SAKSΝAPAAT SAVYSPXLCP ATAAGVLPPA 151 SE* m279/a279 ORFs 279 and 279.a showed a 88.2% identity in 152 aa overlap 10 20 30 40 50 60 m279.pep ITRICGCLISTVFRASASLSAAGFIRLQWEGTDTGSGRARLAPASLAAAMARPTAAALPA : I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I :: I I I I I I I I I a279 MTXICGCLISTVXRASASLSAAGFMRLQWEGTDTGSGRARLAPASLAASIARSTAAALPA 10 20 30 40 50 60 70 80 90 100 110 120 m279.pep ITICPGELKLTASTTSLWAASAQMALTCSSSKPRIAAIAPTPCGTADCISSARRRTSLTA II I I I I I I I I I I I I I I III: : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a279 ITTCPGELKLTASTTSSCADSAQICFTCSSSKPRIAAIAPTPCGTADCISSARXRTSLTA 70 80 90 100 110 120 130 140 150 m279. pep SAKFΝAPAATSAVYSPRLCPATAAGVLPPASKX III I II I I I I I I I I I 11111111111111:1 a279 SAKSΝAPAATSAVYSPXLCPATAAGVLPPASEX 130 140 150 519 and 519-1 gnm7.seq The following partial DΝA sequence was identified in N meningitidis <SEQ ID 3045>: m519.seq (partial) 1 .. TCCGTTATCG GGCGTATGGA GTTGGACAAA ACGTTTGAAG AACGCGACGA 51 AATCAACAGT ACTGTTGTTG CGGCTTTGGA CGAGGCGGCC GGGgCTTgGG 101 GTGTGAAGGT TTTGCGTTAT GAGATTAAAG ACTTGGTTCC GCCGCAAGAA 151 ATCCTTCGCT CAATGCAGGC GCAAATTACT GCCGAACGCG AAAAACGCGC 201 CCGTATCGCC GAATCCGAAG GTCGTAAAAT CGAACAAATC AACCTTGCCA 251 GTGGTCAGCG CGAAGCCGAA ATCCAACAAT CCGAAGGCGA GGCTCAGGCT 301 GCGGTCAATG CGTCAAATGC CGAGAAAATC GCCCGCATCA ACCGCGCCAA 351 AGGTGAAGCG GAATCCTTGC GCCTTGTTGC CGAAGCCAAT GCCGAAGCCA 401 TCCGTCAAAT TGCCGCCGCC CTTCAAACCC AAGGCGGTGC GGATGCGGTC 451 AATCTGAAGA TTGCGGAACA ATACGTCGCT GCGTTCAACA ATCTTGCCAA 501 AGAAAGCAAT ACGCTGATTA TGCCCGCCAA TGTTGCCGAC ATCGGCAGCC 551 TGATTTCTGC CGGTATGAAA ATTATCGACA GCAGCAAAAC CGCCAAaTAA This corresponds to the amino acid sequence <SEQ ID 3046; ORF 519>: m519.pep (partial) 1 ..SVIGRMELDK TFEERDEINS TWAALDEAA GAWGVKVLRY EIKDLVPPQE 51 ILRSMQAQIT AEREKRARIA ESEGRKIEQI NLASGQREAE IQQSEGEAQA 101 AVNASNAEKI ARINRAKGEA ESLRLVAEAN AEAIRQIAAA LQTQGGADAV 151 NLKIAEQYVA AFNNLAKESN TLIMPANVAD IGSLISAGMK IIDSSKTAK* The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 3047>: g519.seq 1 atggaatttt tcattatctt gttggcagcc gtcgccgttt tcggcttcaa 51 atcctttgtc gtcatccccc agcaggaagt ccacgttgtc gaaaggctcg 101 ggcgtttcca tcgcgccctg acggccggtt tgaatatttt gattcccttt 151 atcgaccgcg tcgcctaccg ccattcgctg aaagaaatcc ctttagacgt 201 acccagccag gtctgcatca cgcgcgataa tacgcaattg actgttgacg 251 gcatcatcta tttccaagta accgatccca aactcgcctc atacggttcg 301 agcaactaca ttatggcaat tacccagctt gcccaaacga cgctgcgttc 351 cgttatcggg cgtatggagt tggacaaaac gtttgaagaa cgcgacgaaa 401 tcaacagtac cgtcgtctcc gccctcgatg aagccgccgg ggcttggggt 451 gtgaaagtcc tccgttacga aatcaaggat ttggttccgc cgcaagaaat 501 ccttcgcgca atgcaggcac aaattaccgc cgaacgcgaa aaacgcgccc 551 gtattgccga atccgaaggc cgtaaaatcg aacaaatcaa ccttgccagt 601 ggtcagcgtg aagccgaaat ccaacaatcc gaaggcgagg ctcaggctgc 651 ggtcaatgcg tccaatgccg agaaaatcgc ccgcatcaac cgcgccaaag 701 gcgaagcgga atccctgcgc cttgttgccg aagccaatgc cgaagccaac 751 cgtcaaattg ccgccgccct tcaaacccaa agcggggcgg atgcggtcaa 801 tctgaagatt gcgggacaat acgttaccgc gttcaaaaat cttgccaaag 851 aagacaatac gcggattaag cccgccaagg ttgccgaaat cgggaaccct 901 aattttcggc ggcatgaaaa attttcgcca gaagcaaaaa cggccaaata 951 a This corresponds to the amino acid sequence <SEQ ID 3048; ORF 519.ng>: g519.pep 1 MEFFIILLAA VAVFGFKSFV VIPQQEVHW ERLGRFHRAL TAGLΝILIPF 51 IDRVAYRHSL KEIPLDVPSQ VCITRDΝTQL TVDGIIYFQV TDPKLASYGS 101 SΝYIMAITQL AQTTLRSVIG RMELDKTFEE RDEIΝSTWS ALDEAAGAWG 151 VKVLRYEIKD LVPPQEILRA MQAQITAERE KRARIAESEG RKIEQIΝLAS 201 GQREAEIQQS EGEAQAAV A SΝAEKIARIΝ RAKGEAESLR LVAEAΝAEAΝ 251 RQIAAALQTQ SGADAVΝLKI AGQYVTAFKΝ LAKEDΝTRIK PAKVAEIGΝP 301 ΝFRRHEKFSP EAKTAK* ORF 519 shows 87.5% identity over a 200 aa overlap with a predicted ORF (ORF 519.ng) from N gonorrhoeae: 10 20 30 m519 . pep SVIGRMELDKTFEERDEIΝSTWAALDEAA I I M I I I I M I I I I M I I I I I I l l l l l l g519 YFQVTDPKLASYGSSΝYIMAITQLAQTTLRSVIGRMELDKTFEERDEIΝSTWSALDEAA 90 100 110 120 130 140 40 50 60 70 80 90 m519.pep GAWGVKVLRYEIKDLVPPQEILRSMQAQITAEREKRARIAESEGRKIEQIΝLASGQREAE g519 GA GVKVLRYEIKDLVPPQEILRAMQAQITAEREKRARIAESEGRKIEQINLASGQREAE 150 160 170 180 190 200 100 110 120 130 140 150 m519. ep IQQSEGEAQAAVNASNAEKIARINRAKGEAESLRLVAEA AEAIRQIAAALQTQGGADAV II 11111111 = 11111 g519 IQQSEGEAQAAVNASNAEKIARINRAKGEAESLRLVAEANAEANRQIAAALQTQSGADAV 210 220 230 240 250 260 160 170 180 190 200 m519.pep NLKIAEQYVAAF NLAKESNTLIMPANVADIGSL-ISAGMKIIDSSKTAK M i l l 1 1 1 = 1 1 = 1 1 1 1 1 = 1 1 I 1 1 = 1 1 = 1 1 = = 1 = = 1 1 1 1 g519 NLKIAGQYVTAFK LAKEDNTRIKPAKVAEIGNPNFRRHEKFSPEAKTAK 270 280 290 300 310 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 3049>: a519.seq 1 ATGGAATTTT TCATTATCTT GCTGGCAGCC GTCGTTGTTT TCGGCTTCAA 51 ATCCTTTGTT GTCATCCCAC AGCAGGAAGT CCACGTTGTC GAAAGGCTCG 101 GGCGTTTCCA TCGCGCCCTG ACGGCCGGTT TGAATATTTT GATTCCCTTT 151 ATCGACCGCG TCGCCTACCG CCATTCGCTG AAAGAAATCC CTTTAGACGT 201 ACCCAGCCAG GTCTGCATCA CGCGCGACAA TACGCAGCTG ACTGTTGACG 251 GTATCATCTA TTTCCAAGTA ACCGACCCCA AACTCGCCTC ATACGGTTCG 301 AGCAACTACA TTATGGCGAT TACCCAGCTT GCCCAAACGA CGCTGCGTTC 351 CGTTATCGGG CGTATGGAAT TGGACAAAAC GTTTGAAGAA CGCGACGAAA 401 TCAACAGCAC CGTCGTCTCC GCCCTCGATG AAGCCGCCGG AGCTTGGGGT 451 GTGAAGGTTT TGCGTTATGA GATTAAAGAC TTGGTTCCGC CGCAAGAAAT 501 CCTTCGCTCA ATGCAGGCGC AAATTACTGC TGAACGCGAA AAACGCGCCC 551 GTATCGCCGA ATCCGAAGGT CGTAAAATCG AACAAATCAA CCTTGCCAGT 601 GGTCAGCGCG AAGCCGAAAT CCAACAATCC GAAGGCGAGG CTCAGGCTGC 651 GGTCAATGCG TCAAATGCCG AGAAAATCGC CCGCATCAAC CGCGCCAAAG 701 GTGAAGCGGA ATCCTTGCGC CTTGTTGCCG AAGCCAATGC CGAAGCCATC 751 CGTCAAATTG CCGCCGCCCT TCAAACCCAA GGCGGTGCGG ATGCGGTCAA 801 TCTGAAGATT GCGGAACAAT ACGTCGCCGC GTTCAACAAT CTTGCCAAAG 851 AAAGCAATAC GCTGATTATG CCCGCCAATG TTGCCGACAT CGGCAGCCTG 901 ATTTCTGCCG GTATGAAAAT TATCGACAGC AGCAAAACCG CCAAATAA This corresponds to the amino acid sequence <SEQ ID 3050; ORF 519.a>: a519.pep 1 MEFFIILLAA VVVFGFKSFV VIPQQEVHVV ERLGRFHRAL TAGLΝILIPF 51 IDRVAYRHSL KEIPLDVPSQ VCITRDΝTQL TVDGIIYFQV TDPKLASYGS 101 SΝYIMAITQL AQTTLRSVIG RMELDKTFEE RDEIΝSTWS ALDEAAGA G 151 VKVLRYEIKD LVPPQEILRS MQAQITAERE KRARIAESEG RKIEQIΝLAS 201 GQREAEIQQS EGEAQAAVΝA SΝAEKIARIΝ RAKGEAESLR LVAEAΝAEAI 251 RQIAAALQTQ GGADAVΝLKI AEQYVAAFΝΝ LAKESΝTLIM PAΝVADIGSL 301 ISAGMKIIDS SKTAK* m519/a519 ORFs 519 and 519. a showed a 99.5% identity in 199 aa overlap 10 20 30 519.pep SVIGR ELDKTFEERDEIΝSTWAALDEAA I I I I I I I I I I I II I I I I I I I I I I : I I I I I I a519 YFQVTDPKLASYGSSΝYIMAITQLAQTTLRSVIGRMELDKTFEERDEIΝSTVVSALDEAA 90 100 110 120 130 140 40 50 60 70 80 90 m519.pep GAWGVKVLRYEIKDLVPPQEILRSMQAQITAEREKRARIAESEGRKIEQIΝLASGQREAE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a519 GAWGVKVLRYEIKDLVPPQEILRSMQAQITAEREKRARIAESEGRKIEQIΝLASGQREAE 150 160 170 180 190 200 100 110 120 130 140 150 m519. pep IQQSEGEAQAAVNASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I a519 IQQSEGEAQAAVNASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAV 210 220 230 240 250 260 160 170 180 190 200 m519.pep NLKIAEQYVAAFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAKX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I a519 NLKIAEQYVAAFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAKX 270 280 290 300 310 Further work revealed the DNA sequence identified in N. meningitidis <SEQ ID 3051>: m519-l.seq 1 ATGGAATTTT TCATTATCTT GTTGGTAGCC GTCGCCGTTT TCGGTTTCAA 51 ATCCTTTGTT GTCATCCCAC AACAGGAAGT CCACGTTGTC GAAAGGCTGG 101 GGCGTTTCCA TCGCGCCCTG ACGGcCGGTT TGAATATTTT GATTCCCTTT 151 ATCGACCGCG TCGCCTACCG CCATTCGCTG AAAGAAATCC CTTTAGACGT 201 ACCCAGCCAG GTCTGCATCA CGCGCGACAA TACGCAGCTG ACTGTTGACG 251 GCATCATCTA TTTCCAAGTA ACCGACCCCA AACTCGCCTC ATACGGTTCG 301 AGCAACTACA TTATGGCGAT TACCCAGCTT GCCCAAACGA CGCTGCGTTC 351 CGTTATCGGG CGTATGGAGT TGGACAAAAC GTTTGAAGAA CGCGACGAAA 401 TCAACAGTAC TGTTGTTGCG GCTTTGGACG AGGCGGCCGG GGCTTGGGGT 451 GTGAAGGTTT TGCGTTATGA GATTAAAGAC TTGGTTCCGC CGCAAGAAAT 501 CCTTCGCTCA ATGCAGGCGC AAATTACTGC CGAACGCGAA AAACGCGCCC 551 GTATCGCCGA ATCCGAAGGT CGTAAAATCG AACAAATCAA CCTTGCCAGT 601 GGTCAGCGCG AAGCCGAAAT CCAACAATCC GAAGGCGAGG CTCAGGCTGC 651 GGTCAATGCG TCAAATGCCG AGAAAATCGC CCGCATCAAC CGCGCCAAAG 701 GTGAAGCGGA ATCCTTGCGC CTTGTTGCCG AAGCCAATGC CGAAGCCATC 751 CGTCAAATTG CCGCCGCCCT TCAAACCCAA GGCGGTGCGG ATGCGGTCAA 801 TCTGAAGATT GCGGAACAAT ACGTCGCTGC GTTCAACAAT CTTGCCAAAG 851 AAAGCAATAC GCTGATTATG CCCGCCAATG TTGCCGACAT CGGCAGCCTG 901 ATTTCTGCCG GTATGAAAAT TATCGACAGC AGCAAAACCG CCAAATAA This corresponds to the amino acid sequence <SEQ ID 3052; ORF 519-1>: m519-l. 1 MEFFIILLVA VAVFGFKSFV VIPQQEVHVV ERLGRFHRAL TAGLΝILIPF 51 IDRVAYRHSL KEIPLDVPSQ VCITRDΝTQL TVDGIIYFQV TDPKLASYGS 101 SΝYIMAITQL AQTTLRSVIG RMELDKTFEE RDEIΝSTWA ALDEAAGAWG 151 VKVLRYEIKD LVPPQEILRS MQAQITAERE KRARIAESEG RKIEQIΝLAS 201 GQREAEIQQS EGEAQAAVΝA SΝAEKIARIΝ RAKGEAESLR LVAEAΝAEAI 251 RQIAAALQTQ GGADAVΝLKI AEQYVAAFΝΝ LAKESΝTLIM PAΝVADIGSL 301 ISAGMKIIDS SKTAK* The following DΝA sequence was identified in N gonorrhoeae <SEQ ID 3053>: g519-l.seq 1 ATGGAATTTT TCATTATCTT GTTGGCAGCC GTCGCCGTTT TCGGCTTCAA 51 ATCCTTTGTC GTCATCCCCC AGCAGGAAGT CCACGTTGTC GAAAGGCTCG 101 GGCGTTTCCA TCGCGCCCTG ACGGCCGGTT TGAATATTTT GATTCCCTTT 151 ATCGACCGCG TCGCCTACCG CCATTCGCTG AAAGAAATCC CTTTAGACGT 201 ACCCAGCCAG GTCTGCATCA CGCGCGATAA TACGCAATTG ACTGTTGACG 251 GCATCATCTA TTTCCAAGTA ACCGATCCCA AACTCGCCTC ATACGGTTCG 301 AGCAACTACA TTATGGCAAT TACCCAGCTT GCCCAAACGA CGCTGCGTTC 351 CGTTATCGGG CGTATGGAGT TGGACAAAAC GTTTGAAGAA CGCGACGAAA 401 TCAACAGTAC CGTCGTCTCC GCCCTCGATG AAGCCGCCGG GGCTTGGGGT 451 GTGAAAGTCC TCCGTTACGA AATCAAGGAT TTGGTTCCGC CGCAAGAAAT 501 CCTTCGCGCA ATGCAGGCAC AAATTACCGC CGAACGCGAA AAACGCGCCC 551 GTATTGCCGA ATCCGAAGGC CGTAAAATCG AACAAATCAA CCTTGCCAGT 601 GGTCAGCGTG AAGCCGAAAT CCAACAATCC GAAGGCGAGG CTCAGGCTGC 651 GGTCAATGCG TCCAATGCCG AGAAAATCGC CCGCATCAAC CGCGCCAAAG 701 GCGAAGCGGA ATCCCTGCGC CTTGTTGCCG AAGCCAATGC CGAAGCCATC 751 CGTCAAATTG CCGCCGCCCT TCAAACCCAA GGCGGGGCGG ATGCGGTCAA 801 TCTGAAGATT GCGGAACAAT ACGTAGCCGC GTTCAACAAT CTTGCCAAAG 851 AAAGCAATAC GCTGATTATG CCCGCCAATG TTGCCGACAT CGGCAGCCTG 901 ATTTCTGCCG GCATGAAAAT TATCGACAGC AGCAAAACCG CCAAATAA This corresponds to the amino acid sequence <SEQ ID 3054; ORF 519-l.ng>: g519-l.pep 1 MEFFIILLAA VAVFGFKSFV VIPQQEVHVV ERLGRFHRAL TAGLNILIPF 51 IDRVAYRHSL KEIPLDVPSQ VCITRDNTQL TVDGIIYFQV TDPKLASYGS 101 SNYIMAITQL AQTTLRSVIG RMELDKTFEE RDEINSTVVS ALDEAAGAWG 151 VKVLRYEIKD LVPPQEILRA MQAQITAERE KRARIAESEG RKIEQINLAS 201 GQREAEIQQS EGEAQAAVNA SNAEKIARIN RAKGEAESLR LVAEANAEAI 251 RQIAAALQTQ GGADAVNLKI AEQYVAAFNN LAKESNTLIM PANVADIGSL 301 ISAGMKIIDS SKTAK* m519-l/g519-l ORFs 519-1 and 519-1. ng showed a 99.0% identity in 315 aa overlap 10 20 30 40 50 60 g519-l.pep MEFFIILLAAVAVFGFKSFWIPQQEVHWERLGRFHRALTAGLNILIPFIDRVAYRHSL I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I m519-l MEFFIILLVAVAVFGFKSFWIPQQEVHVVERLGRFHRALTAGLNILIPFIDRVAYRHSL 10 20 30 40 50 60 70 80 90 100 110 120 g519-l.pep KEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGΞSNYIMAITQLAQTTLRSVIG I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I m519- l KEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIG 70 80 90 100 110 120 130 140 150 160 170 180 g519-l . pep RMELDKTFEERDEINSTWSALDEAAGAWGVKVLRYEIKDLVPPQEILRAMQAQITAERE I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I ιτι519-l RMELDKTFEERDEINSTVVAALDEAAGAWGVKVLRYEIKDLVPPQEILRSMQAQITAERE 130 140 150 160 170 180 190 200 210 220 230 240 g519-l.pep KRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I 519-l KRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR 190 200 210 220 230 240 250 260 270 280 290 300 g519-1. pep LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m519-1 LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL 250 260 270 280 290 300 310 g519-l.pep ISAGMKIIDSSKTAKX I I I I I I I I II I I I I I I m519-l ISAGMKIIDSSKTAKX 310 The following DNA sequence was identified in N meningitidis <SEQ ID 3055>: a519-l. seq 1 ATGGAATTTT TCATTATCTT GCTGGCAGCC GTCGTTGTTT TCGGCTTCAA 51 ATCCTTTGTT GTCATCCCAC AGCAGGAAGT CCACGTTGTC GAAAGGCTCG 101 GGCGTTTCCA TCGCGCCCTG ACGGCCGGTT TGAATATTTT GATTCCCTTT 151 ATCGACCGCG TCGCCTACCG CCATTCGCTG AAAGAAATCC CTTTAGACGT 201 ACCCAGCCAG GTCTGCATCA CGCGCGACAA TACGCAGCTG ACTGTTGACG 251 GTATCATCTA TTTCCAAGTA ACCGACCCCA AACTCGCCTC ATACGGTTCG 301 AGCAACTACA TTATGGCGAT TACCCAGCTT GCCCAAACGA CGCTGCGTTC 351 CGTTATCGGG CGTATGGAAT TGGACAAAAC GTTTGAAGAA CGCGACGAAA 401 TCAACAGCAC CGTCGTCTCC GCCCTCGATG AAGCCGCCGG AGCTTGGGGT 451 GTGAAGGTTT TGCGTTATGA GATTAAAGAC TTGGTTCCGC CGCAAGAAAT 501 CCTTCGCTCA ATGCAGGCGC AAATTACTGC TGAACGCGAA AAACGCGCCC 551 GTATCGCCGA ATCCGAAGGT CGTAAAATCG AACAAATCAA CCTTGCCAGT 601 GGTCAGCGCG AAGCCGAAAT CCAACAATCC GAAGGCGAGG CTCAGGCTGC 651 GGTCAATGCG TCAAATGCCG AGAAAATCGC CCGCATCAAC CGCGCCAAAG 701 GTGAAGCGGA ATCCTTGCGC CTTGTTGCCG AAGCCAATGC CGAAGCCATC 751 CGTCAAATTG CCGCCGCCCT TCAAACCCAA GGCGGTGCGG ATGCGGTCAA 801 TCTGAAGATT GCGGAACAAT ACGTCGCCGC GTTCAACAAT CTTGCCAAAG 851 AAAGCAATAC GCTGATTATG CCCGCCAATG TTGCCGACAT CGGCAGCCTG 901 ATTTCTGCCG GTATGAAAAT TATCGACAGC AGCAAAACCG CCAAATAA This corresponds to the amino acid sequence <SEQ ID 3056; ORF 519-l.a>: a519-l.pep. 1 MEFFIILLAA VVVFGFKSFV VIPQQEVHVV ERLGRFHRAL TAGLNILIPF 51 IDRVAYRHSL KEIPLDVPSQ VCITRDNTQL TVDGIIYFQV TDPKLASYGS 101 SNYIMAITQL AQTTLRSVIG RMELDKTFEE RDEINSTVVS ALDEAAGAWG 151 VKVLRYEIKD LVPPQEILRS MQAQITAERE KRARIAESEG RKIEQINLAS 201 GQREAEIQQS EGEAQAAVNA SNAEKIARIN RAKGEAESLR LVAEANAEAI 251 RQIAAALQTQ GGADAVNLKI AEQYVAAFNN LAKESNTLIM PANVADIGSL 301 ISAGMKIIDS SKTAK* m519-l/a519-l ORFs 519-1 and 519-1. a showed a 99.0% identity in 315 aa overlap 10 20 30 40 50 60 a519-l.pep MEFFIILLAAVVVFGFKSFVVIPQQEVHWERLGRFHRALTAGLNILIPFIDRVAYRHSL I I I I I I I I : I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I m519-l MEFFIILLVAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGLNILIPFIDRVAYRHSL 10 20 30 40 50 60 70 80 90 100 110 120 a519-l.pep KEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIG I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m519-l KEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSΞNYIMAITQLAQTTLRSVIG 70 80 90 100 110 120 130 140 150 160 170 180 a519-l.pep RMELDKTFEERDEINSTVVSALDEAAGAWGVKVLRYEIKDLVPPQEILRSMQAQITAERE I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m519-l RMELDKTFEERDEINSTVVAALDEAAGAWGVKVLRYEIKDLVPPQEILRSMQAQITAERE 130 140 150 160 170 180 190 200 210 220 230 240 a519-l.pep KRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m519-l KRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR 190 200 210 220 230 240 250 260 270 280 290 300 a519-l.pep LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m519-l LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL 250 260 270 280 290 300 310 a519-l.pep ISAGMKIIDSSKTAKX I I I I I I I I I I I I I I I I 519-l ISAGMKIIDSSKTAKX 310 576 and 576-1 gnm22.seq The following partial DNA sequence was identified in N meningitidis <SEQ ID 3057>: m576.seq.. (partial) 1 ..ATGCAGCAGG CAAGCTATGC GATGGGCGTG GACATCGGAC GCTCCCTGAA 51 GCAAATGAAG GAACAGGGCG CGGAAATCGA TTTGAAAGTC TTTACCGAAG 101 CCATGCAGGC AGTGTATGAC GGCAAAGAAA TCAAAATGAC CGAAGAGCAG 151 GCTCAGGAAG TCATGATGAA ATTCCTTCAG GAACAACAGG CTAAAGCCGT 201 AGAAAAACAC AAGGCGGACG CGAAGGCCAA TAAAGAAAAA GGCGAAGCCT 251 TTCTGAAAGA AAATGCCGCC AAAGACGGCG TGAAGACCAC TGCTTCCGGC 301 CTGCAATACA AAATCACCAA ACAGGGCGAA GGCAAACAGC CGACCAAAGA 351 CGACATCGTT ACCGTGGAAT ACGAAGGCCG CCTGATTGAC GGTACGGTAT 401 TCGACAGCAG CAAAGCCAAC GGCGGCCCGG TCACCTTCCC TTTGAGCCAA 451 GTGATTCCGG GTTGGACCGA AGgCGTACAG CTTCTGAAAG AAGGCGGCGA 501 AGCCACGTTC TACATCCCGT CCAACCTTGC CTACCGCGAA CAGGGTGCGG 551 GCGACAAAAT CGGTCCGAAC GCCACTTTGG TATTTGATGT GAAACTGGTC 601 AAAATCGGCG CACCCGAAAA CGCGCCCGCC AAGCAGCCGG CTCAAGTCGA 651 CATCAAAAAA GTAAATTAA This corresponds to the amino acid sequence <SEQ ID 3058; ORF 576>: m576.pep.. (partial) 1 ..MQQASYAMGV DIGRSLKQMK EQGAEIDLKV FTEAMQAVYD GKEIKMTEEQ 51 AQEVMMKFLQ EQQAKAVEKH KADAKAΝKEK GEAFLKEΝAA KDGVKTTASG 101 LQYKITKQGE GKQPTKDDIV TVEYEGRLID GTVFDSSKAΝ GGPVTFPLSQ 151 VIPGWTEGVQ LLKEGGEATF YIPSΝLAYRE QGAGDKIGPΝ ATLVFDVKLV 201 KIGAPEΝAPA KQPAQVDIKK VΝ* The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 3059>: g576. seq. (partial) 1 ..atgggcgtgg acatcggacg ctccctgaaa caaatgaagg aacagggcgc 51 ggaaatcgat ttgaaagtct ttaccgatgc catgcaggca gtgtatgacg 101 gcaaagaaat caaaatgacc gaagagcagg cccaggaagt gatgatgaaa 151 ttcctgcagg agcagcaggc taaagccgta gaaaaacaca aggcggatgc 201 gaaggccaac aaagaaaaag gcgaagcctt cctgaaggaa aatgccgccg 251 aagacggcgt gaagaccact gcttccggtc tgcagtacaa aatcaccaaa 301 cagggtgaag gcaaacagcc gacaaaagac gacatcgtta ccgtggaata 351 cgaaggccgc ctgattgacg gtaccgtatt cgacagcagc aaagccaacg 401 gcggcccggc caccttccct ttgagccaag tgattccggg ttggaccgaa 451 ggcgtacggc ttctgaaaga aggcggcgaa gccacgttct acatcccgtc 501 caaccttgcc taccgcgaac agggtgcggg cgaaaaaatc ggtccgaacg 551 ccactttggt atttgacgtg aaactggtca aaatcggcgc acccgaaaac 601 gcgcccgcca agcagccgga tcaagtcgac atcaaaaaag taaattaa This corresponds to the amino acid sequence <SEQ ID 3060; ORF 576.ng>: g576.pep.. (partial) 1 ..MGVDIGRSLK QMKEQGAEID LKVFTDAMQA VYDGKEIKMT EEQAQEVMMK 51 FLQEQQAKAV EKHKADAKAΝ KEKGEAFLKE ΝAAEDGVKTT ASGLQYKITK 101 QGEGKQPTKD DIVTVEYEGR LIDGTVFDSS KAΝGGPATFP LSQVIPGWTE 151 GVRLLKEGGE ATFYIPSΝLA YREQGAGEKI GPΝATLVFDV KLVKIGAPEΝ 201 APAKQPDQVD IKKVΝ* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m576/g576 ORFs 576 and 576. ng showed a 97.2% identity in 215 aa overlap 10 20 30 40 50 60 576. pep MQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQEVMMKFLQ I I I I I I I I I II I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I g576 MGVDIGRSLKQMKEQGAEIDLKVFTDAMQAVYDGKEIKMTEEQAQEVMMKFLQ 10 20 30 40 50 70 80 90 100 110 120 m576. pep EQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGEGKQPTKDDIV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g576 EQQAKAVEKHKADAKANKEKGEAFLKENAAEDGVKTTASGLQYKITKQGEGKQPTKDDIV 60 70 80 90 100 110 130 140 150 160 170 180 m576.pep TVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEATFYIPSNLAYRE I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I g576 TVEYEGRLIDGTVFDSSKANGGPATFPLSQVIPGWTEGVRLLKEGGEATFYIPSNLAYRE 120 130 140 150 160 170 190 200 210 220 m576. pep QGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVNX I I II : I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I g576 QGAGEKIGPNATLVFDVKLVKIGAPENAPAKQPDQVDIKKVNX 180 190 200 210 The following partial DNA sequence was identified in N meningitidis <SEQ ID 3061>: a576.seq 1 ATGAACACCA TTTTCAAAAT CAGCGCACTG ACCCTTTCCG CCGCTTTGGC 51 ACTTTCCGCC TGCGGCAAAA AAGAAGCCGC CCCCGCATCT GCATCCGAAC 101 CTGCCGCCGC TTCTTCCGCG CAGGGCGACA CCTCTTCGAT CGGCAGCACG 151 ATGCAGCAGG CAAGCTATGC GATGGGCGTG GACATCGGAC GCTCCCTGAA 201 GCAAATGAAG GAACAGGGCG CGGAAATCGA TTTGAAAGTC TTTACCGAAG 251 CCATGCAGGC AGTGTATGAC GGCAAAGAAA TCAAAATGAC CGAAGAGCAG 301 GCTCAGGAAG TCATGATGAA ATTCCTTCAG GAACAACAGG CTAAAGCCGT 351 AGAAAAACAC AAGGCGGACG CGAAGGCCAA TAAAGAAAAA GGCGAAGCCT 401 TTCTGAAAGA AAATGCCGCC AAAGACGGCG TGAAGACCAC TGCTTCCGGC 451 CTGCAATACA AAATCACCAA ACAGGGCGAA GGCAAACAGC CGACCAAAGA 501 CGACATCGTT ACCGTGGAAT ACGAAGGCCG CCTGATTGAC GGTACGGTAT 551 TCGACAGCAG CAAAGCCAAC GGCGGCCCGG TCACCTTCCC TTTGAGCCAA 601 GTGATTCTGG GTTGGACCGA AGGCGTACAG CTTCTGAAAG AAGGCGGCGA 651 AGCCACGTTC TACATCCCGT CCAACCTTGC CTACCGCGAA CAGGGTGCGG 701 GCGACAAAAT CGGCCCGAAC GCCACTTTGG TATTTGATGT GAAACTGGTC 751 AAAATCGGCG CACCCGAAAA CGCGCCCGCC AAGCAGCCGG CTCAAGTCGA 801 CATCAAAAAA GTAAATTAA This corresponds to the amino acid sequence <SEQ ID 3062; ORF 576. a>: a576.pep 1 MΝTIFKISAL TLSAALALSA CGKKEAAPAS ASEPAAASSA QGDTSSIGST 51 MQQASYAMGV DIGRSLKQMK EQGAEIDLKV FTEAMQAVYD GKEIKMTEEQ 101 AQEVMMKFLQ EQQAKAVEKH KADAKAΝKEK GEAFLKEΝAA KDGVKTTASG 151 LQYKITKQGE GKQPTKDDIV TVEYEGRLID GTVFDSSKAΝ GGPVTFPLSQ 201 VILGWTEGVQ LLKEGGEATF YIPSΝLAYRE QGAGDKIGPΝ ATLVFDVKLV 251 KIGAPEΝAPA KQPAQVDIKK VΝ* 576/a576 ORFs 576 and 576. a showed a 99.5% identity in 222 aa overlap 10 20 30 m576. pep MQQASYAMGVDIGRSLKQMKEQGAEIDLKV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a576 CGKKEAAPASASEPAAASSAQGDTSSIGSTMQQASYAMGVDIGRSLKQMKEQGAEIDLKV 30 40 50 60 70 80 40 50 60 70 80 90 m576. pep FTEAMQAVYDGKEIKMTEEQAQEVMMKFLQEQQAKAVEKHKADAKAΝKEKGEAFLKEΝAA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M I I a576 FTEAMQAVYDGKEIKMTEEQAQEVMMKFLQEQQAKAVEKHKADAKAΝKEKGEAFLKEΝAA 90 100 110 120 130 140 100 110 120 130 140 150 m576.pep KDGVKTTASGLQYKITKQGEGKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a576 KDGVKTTASGLQYKITKQGEGKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQ 150 160 170 180 190 200 160 170 180 190 200 210 m576. pep VIPGWTEGVQLLKEGGEATFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPA II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a576 VILGWTEGVQLLKEGGEATFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPA 210 220 230 240 250 260 220 m576.pep KQPAQVDIKKVNX I I I I I I I I I I I I I a576 KQPAQVDIKKVNX 270 Further work revealed the DNA sequence identified in N meningitidis <SEQ ID 3063>: 576-l . seq 1 ATGAACACCA TTTTCAAAAT CAGCGCACTG ACCCTTTCCG CCGCTTTGGC 51 ACTTTCCGCC TGCGGCAAAA AAGAAGCCGC CCCCGCATCT GCATCCGAAC 101 CTGCCGCCGC TTCTTCCGCG CAGGGCGACA CCTCTTCGAT CGGCAGCACG 151 ATGCAGCAGG CAAGCTATGC GATGGGCGTG GACATCGGAC GCTCCCTGAA 201 GCAAATGAAG GAACAGGGCG CGGAAATCGA TTTGAAAGTC TTTACCGAAG 251 CCATGCAGGC AGTGTATGAC GGCAAAGAAA TCAAAATGAC CGAAGAGCAG 301 GCTCAGGAAG TCATGATGAA ATTCCTTCAG GAACAACAGG CTAAAGCCGT 351 AGAAAAACAC AAGGCGGACG CGAAGGCCAA TAAAGAAAAA GGCGAAGCCT 401 TTCTGAAAGA AAATGCCGCC AAAGACGGCG TGAAGACCAC TGCTTCCGGC 451 CTGCAATACA AAATCACCAA ACAGGGCGAA GGCAAACAGC CGACCAAAGA 501 CGACATCGTT ACCGTGGAAT ACGAAGGCCG CCTGATTGAC GGTACGGTAT 551 TCGACAGCAG CAAAGCCAAC GGCGGCCCGG TCACCTTCCC TTTGAGCCAA 601 GTGATTCCGG GTTGGACCGA AGGCGTACAG CTTCTGAAAG AAGGCGGCGA 651 AGCCACGTTC TACATCCCGT CCAACCTTGC CTACCGCGAA CAGGGTGCGG 701 GCGACAAAAT CGGTCCGAAC GCCACTTTGG TATTTGATGT GAAACTGGTC 751 AAAATCGGCG CACCCGAAAA CGCGCCCGCC AAGCAGCCGG CTCAAGTCGA 801 CATCAAAAAA GTAAATTAA This corresponds to the amino acid sequence <SEQ ID 3064; ORF 576- 1>: m576-l.pep 1 MΝTIFKISAL TLSAALALSA CGKKEAAPAS ASEPAAASSA QGDTSSIGST 51 MQQASYAMGV DIGRSLKQMK EQGAEIDLKV FTEAMQAVYD GKEIKMTEEQ 101 AQEVMMKFLQ EQQAKAVEKH KADAKAΝKEK GEAFLKEΝAA KDGVKTTASG 151 LQYKITKQGE GKQPTKDDIV TVEYEGRLID GTVFDSSKAΝ GGPVTFPLSQ 201 VIPGWTEGVQ LLKEGGEATF YIPSΝLAYRE QGAGDKIGPΝ ATLVFDVKLV 251 KIGAPEΝAPA KQPAQVDIKK VΝ* The following DΝA sequence was identified in N gonorrhoeae <SEQ ID 3065>: g576-l. seq 1 ATGAACACCA TTTTCAAAAT CAGCGCACTG ACCCTTTCCG CCGCTTTGGC 51 ACTTTCCGCC TGCGGCAAAA AAGAAGCCGC CCCCGCATCT GCATCCGAAC 101 CTGCCGCCGC TTCTGCCGCG CAGGGCGACA CCTCTTCAAT CGGCAGCACG 151 ATGCAGCAGG CAAGCTATGC AATGGGCGTG GACATCGGAC GCTCCCTGAA 201 ACAAATGAAG GAACAGGGCG CGGAAATCGA TTTGAAAGTC TTTACCGATG 251 CCATGCAGGC AGTGTATGAC GGCAAAGAAA TCAAAATGAC CGAAGAGCAG 301 GCCCAGGAAG TGATGATGAA ATTCCTGCAG GAGCAGCAGG CTAAAGCCGT 351 AGAAAAACAC AAGGCGGATG CGAAGGCCAA CAAAGAAAAA GGCGAAGCCT 401 TCCTGAAGGA AAATGCCGCC AAAGACGGCG TGAAGACCAC TGCTTCCGGT 451 CTGCAGTACA AAATCACCAA ACAGGGTGAA GGCAAACAGC CGACAAAAGA 501 CGACATCGTT ACCGTGGAAT ACGAAGGCCG CCTGATTGAC GGTACCGTAT 551 TCGACAGCAG CAAAGCCAAC GGCGGCCCGG CCACCTTCCC TTTGAGCCAA 601 GTGATTCCGG GTTGGACCGA AGGCGTACGG CTTCTGAAAG AAGGCGGCGA 651 AGCCACGTTC TACATCCCGT CCAACCTTGC CTACCGCGAA CAGGGTGCGG 701 GCGAAAAAAT CGGTCCGAAC GCCACTTTGG TATTTGACGT GAAACTGGTC 751 AAAATCGGCG CACCCGAAAA CGCGCCCGCC AAGCAGCCGG ATCAAGTCGA 801 CATCAAAAAA GTAAATTAA This corresponds to the amino acid sequence <SEQ ID 3066; ORF 576-1. ng>: g576-l.pep 1 MNTIFKISAL TLSAALALSA CGKKEAAPAS ASEPAAASAA QGDTSSIGST 51 MQQASYAMGV DIGRSLKQMK EQGAEIDLKV FTDAMQAVYD GKEIKMTEEQ 101 AQEVMMKFLQ EQQAKAVEKH KADAKANKEK GEAFLKENAA KDGVKTTASG 151 LQYKITKQGE GKQPTKDDIV TVEYEGRLID GTVFDSSKAN GGPATFPLSQ 201 VIPGWTEGVR LLKEGGEATF YIPSNLAYRE QGAGEKIGPN ATLVFDVKLV 251 KIGAPENAPA KQPDQVDIKK VN* g576-l/m576-l ORFs 576-1 and 576-1. ng showed a 97.8% identity in 272 aa overlap 10 20 30 40 50 60 g576-l.pep MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASAAQGDTSSIGSTMQQASYAMGV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I II I I I I I I I m576-l MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASSAQGDTSSIGSTMQQASYAMGV 10 20 30 40 50 60 70 80 90 100 110 120 g576-1. pep DIGRSLKQMKEQGAEIDLKVFTDAMQAVYDGKEIKMTEEQAQEVMMKFLQEQQAKAVEKH I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I m576-l DIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQEVMMKFLQEQQAKAVEKH 70 80 90 100 110 120 130 140 150 160 170 180 g576-1. pep KADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGEGKQPTKDDIVTVEYEGRLID I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m576-l KADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGEGKQPTKDDIVTVEYEGRLID 130 140 150 160 170 180 190 200 210 220 230 240 g576-1. pep GTVFDSSKANGGPATFPLSQVIPGWTEGVRLLKEGGEATFYIPSNLAYREQGAGEKIGPN I I I I I I I I I I I I I : I I I I II I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I m576-l GTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEATFYIPSNLAYREQGAGDKIGPN 190 200 210 220 230 240 250 260 270 g576-l .pep ATLVFDVKLVKIGAPENAPAKQPDQVDIKKVNX I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I m576-1 ATLVFDVKLVKIGAPENAPAKQPAQVDIKKVNX 250 260 270 The following DNA sequence was identified in N meningitidis <SEQ ID 3067>: a576-l.seq 1 ATGAACACCA TTTTCAAAAT CAGCGCACTG ACCCTTTCCG CCGCTTTGGC 51 ACTTTCCGCC TGCGGCAAAA AAGAAGCCGC CCCCGCATCT GCATCCGAAC 101 CTGCCGCCGC TTCTTCCGCG CAGGGCGACA CCTCTTCGAT CGGCAGCACG 151 ATGCAGCAGG CAAGCTATGC GATGGGCGTG GACATCGGAC GCTCCCTGAA 201 GCAAATGAAG GAACAGGGCG CGGAAATCGA TTTGAAAGTC TTTACCGAAG 251 CCATGCAGGC AGTGTATGAC GGCAAAGAAA TCAAAATGAC CGAAGAGCAG 301 GCTCAGGAAG TCATGATGAA ATTCCTTCAG GAACAACAGG CTAAAGCCGT 351 AGAAAAACAC AAGGCGGACG CGAAGGCCAA TAAAGAAAAA GGCGAAGCCT 401 TTCTGAAAGA AAATGCCGCC AAAGACGGCG TGAAGACCAC TGCTTCCGGC 451 CTGCAATACA AAATCACCAA ACAGGGCGAA GGCAAACAGC CGACCAAAGA 501 CGACATCGTT ACCGTGGAAT ACGAAGGCCG CCTGATTGAC GGTACGGTAT 551 TCGACAGCAG CAAAGCCAAC GGCGGCCCGG TCACCTTCCC TTTGAGCCAA 601 GTGATTCTGG GTTGGACCGA AGGCGTACAG CTTCTGAAAG AAGGCGGCGA 651 AGCCACGTTC TACATCCCGT CCAACCTTGC CTACCGCGAA CAGGGTGCGG 701 GCGACAAAAT CGGCCCGAAC GCCACTTTGG TATTTGATGT GAAACTGGTC 751 AAAATCGGCG CACCCGAAAA CGCGCCCGCC AAGCAGCCGG CTCAAGTCGA 801 CATCAAAAAA GTAAATTAA This corresponds to the amino acid sequence <SEQ ID 3068; ORF 576-1. a>: a576-l.pep 1 MNTIFKISAL TLSAALALSA CGKKEAAPAS ASEPAAASSA QGDTSSIGST 51 MQQASYAMGV DIGRSLKQMK EQGAEIDLKV FTEAMQAVYD GKEIKMTEEQ 101 AQEVMMKFLQ EQQAKAVEKH KADAKANKEK GEAFLKENAA KDGVKTTASG 151 LQYKITKQGE GKQPTKDDIV TVEYEGRLID GTVFDSSKAN GGPVTFPLSQ 201 VILGWTEGVQ LLKEGGEATF YIPSNLAYRE QGAGDKIGPN ATLVFDVKLV 251 KIGAPENAPA KQPAQVDIKK VN* a576-l/m576-l ORFs 576-1 and 576-1. a showed a 99.6% identity in 272 aa overlap 10 20 30 40 50 60 a576-l.pep MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASSAQGDTSSIGSTMQQASYAMGV I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I II I I I I I I I I I I I m576-l MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASSAQGDTSSIGSTMQQASYAMGV 10 20 30 40 50 60 70 80 90 100 110 120 a576-1. pep DIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQEVMMKFLQEQQAKAVEKH I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I 576-1 DIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQEVMMKFLQEQQAKAVEKH 70 80 90 100 110 120 130 140 150 160 170 180 a576-1. pep KADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGEGKQPTKDDIVTVEYEGRLID I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m576-1 KADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGEGKQPTKDDIVTVEYEGRLID 130 140 150 160 170 180 190 200 210 220 230 240 a576-l.pep GTVFDSSKANGGPVTFPLSQVILGWTEGVQLLKEGGEATFYIPSNLAYREQGAGDKIGPN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m576-l GTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEATFYIPSNLAYREQGAGDKIGPN 190 200 210 220 230 240 250 260 270 a576-1. pep ATLVFDVKLVKIGAPENAPAKQPAQVDIKKVNX I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I 576-1 ATLVFDVKLVKIGAPENAPAKQPAQVDIKKVNX 250 260 270 919 gnm43.seq The following partial DNA sequence was identified in N. meningitidis <SEQ ID 3069>: m919.seq 1 ATGAAAAAAT ACCTATTCCG CGCCGCCCTG TACGGCATCG CCGCCGCCAT 51 CCTCGCCGCC TGCCAAAGCA AGAGCATCCA AACCTTTCCG CAACCCGACA 101 CATCCGTCAT CAACGGCCCG GACCGGCCGG TCGGCATCCC CGACCCCGCC 151 GGAACGACGG TCGGCGGCGG CGGGGCCGTC TATACCGTTG TACCGCACCT 201 GTCCCTGCCC CACTGGGCGG CGCAGGATTT CGCCAAAAGC CTGCAATCCT 251 TCCGCCTCGG CTGCGCCAAT TTGAAAAACC GCCAAGGCTG GCAGGATGTG 301 TGCGCCCAAG CCTTTCAAAC CCCCGTCCAT TCCTTTCAGG CAAAACAGTT 351 TTTTGAACGC TATTTCACGC CGTGGCAGGT TGCAGGCAAC GGAAGCCTTG 401 CCGGTACGGT TACCGGCTAT TACGAACCGG TGCTGAAGGG CGACGACAGG 451 CGGACGGCAC AAGCCCGCTT CCCGATTTAC GGTATTCCCG ACGATTTTAT 501 CTCCGTCCCC CTGCCTGCCG GTTTGCGGAG CGGAAAAGCC CTTGTCCGCA 551 TCAGGCAGAC GGGAAAAAAC AGCGGCACAA TCGACAATAC CGGCGGCACA 601 CATACCGCCG ACCTCTCCcG ATTCCCCATC ACCGCGCGCA CAACAGCAAT 651 CAAAGGCAGG TTTGAAGGAA GCCGCTTCCT CCCCTACCAC ACGCGCAACC 701 AAATCAACGG CGGCGCGCTT GACGGCAAAG CCCCGATACT CGGTTACGCC 751 GAAGACCCTG TCGAACTTTT TTTTATGCAC ATCCAAGGCT CGGGCCGTCT 801 GAAAACCCCG TCCGGCAAAT ACATCCGCAT CGGCTATGCC GACAAAAACG 851 AACATCCyTA CGTTTCCATC GGACGCTATA TGGCGGATAA GGGCTACCTC 901 AAACTCGGAC AAACCTCCAT GCAGGGCATT AAGTCTTATA TGCGGCAAAA 951 TCCGCAACGC CTCGCCGAAG TTTTGGGTCA AAACCCCAGC TATATCTTTT 1001 TCCGCGAGCT TGCCGGAAGC AGCAATGACG GCCCTGTCGG CGCACTGGGC 1051 ACGCCGCTGA TGGGGGAATA TGCCGGCGCA GTCGACCGGC ACTACATTAC 1101 CTTGGGTGCG CCCTTATTTG TCGCCACCGC CCATCCGGTT ACCCGCAAAG 1151 CCCTCAACCG CCTGATTATG GCGCAGGATA CCGGCAGCGC GATTAAAGGC 1201 GCGGTGCGCG TGGATTATTT TTGGGGATAC GGCGACGAAG CCGGCGAACT 1251 TGCCGGCAAA CAGAAAACCA CGGGATATGT CTGGCAGCTC CTACCCAACG 1301 GTATGAAGCC CGAATACCGc CCGTAA This corresponds to the amino acid sequence <SEQ ID 3070; ORF 919>: m919.pep 1 MKKYLFRAAL YGIAAAILAA CQSKSIQTFP QPDTSVINGP DRPVGIPDPA 51 GTTVGGGGAV YTWPHLSLP HWAAQDFAKS LQSFRLGCAN LKNRQGWQDV 101 CAQAFQTPVH SFQAKQFFER YFTPWQVAGN GSLAGTVTGY YEPVLKGDDR 151 RTAQARFPIY GIPDDFISVP LPAGLRSGKA LVRIRQTGKN SGTIDNTGGT 201 HTADLSRFPI TARTTAIKGR FEGSRFLPYH TRNQINGGAL DGKAPILGYA 251 EDPVELFFMH IQGSGRLKTP SGKYIRIGYA DKNEHPYVSI GRYMADKGYL 301 KLGQTSMQGI KSYMRQNPQR LAEVLGQNPS YIFFRELAGS SNDGPVGALG 351 TPLMGEYAGA VDRHYITLGA PLFVATAHPV TRKALNRLIM AQDTGSAIKG 401 AVRVDYFWGY GDEAGELAGK QKTTGYVWQL LPNGMKPEYR P* The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 3071>: g919. seq 1 ATGAAAAAAC ACCTGCTCCG CTCCGCCCTG TACGGcatCG CCGCCgccAT 51 CctcgCCGCC TGCCAAAgca gGAGCATCCA AACCTTTCCG CAACCCGACA 101 CATCCGTCAT CAACGGCCCG GACCGGCCGG CCGGCATCCC CGACCCCGCC 151 GGAACGACGG TTGCCGGCGG CGGGGCCGTC TATACCGTTG TGCCGCACCT 201 GTCCATGCCC CACTGGGCGG CGCaggATTT TGCCAAAAGC CTGCAATCCT 251 TCCGCCTCGG CTGCGCCAAT TTGAAAAACC GCCAAGGCTG GCAGGATGTG 301 TGCGCCCAAG CCTTTCAAAC CCCCGTGCAT TCCTTTCAGG CAAAGcGgTT 351 TTTTGAACGC TATTTCACGC cgtGGCaggt tgcaggcaAC GGAAGcCTTG 401 Caggtacggt TACCGGCTAT TACGAACCGG TGCTGAAGGG CGACGGCAGG 451 CGGACGGAAC GGGCCCGCTT CCCGATTTAC GGTATTCCCG ACGATTTTAT 501 CTCCGTCCCG CTGCCTGCCG GTTTGCGGGG CGGAAAAAAC CTTGTCCGCA 551 TCAGGCAGac ggGGAAAAAC AGCGGCACGA TCGACAATGC CGGCGGCACG 601 CATACCGCCG ACCTCTCCCG ATTCCCCATC ACCGCGCGCA CAACGGcaat 651 caaaGGCAGG TTTGAaggAA GCCGCTTCCT CCCTTACCAC ACGCGCAACC 701 AAAtcaacGG CGGCgcgcTT GACGGCAAag cccCCATCCT CggttacgcC 751 GAagaccCcG tcgaacttTT TTTCATGCAC AtccaaggCT CGGGCCGCCT 801 GAAAACCCcg tccggcaaat acatCCGCAt cggaTacgcc gacAAAAACG 851 AACAtccgTa tgtttccatc ggACGctaTA TGGCGGACAA AGGCTACCTC 901 AAGctcgggc agACCTCGAT GCAGGgcatc aaagcCTATA TGCGGCAAAA 951 TCCGCAACGC CTCGCCGAAG TTTTGGGTCA AAACCCCAGC TATATCTTTT 1001 TCCGCGAGCT TGCCGGAAGC GGCAATGAGG GCCCCGTCGG CGCACTGGGC 1051 ACGCCACTGA TGGGGGAATA CGCCGGCGCA ATCGACCGGC ACTACATTAC 1101 CTTGGGCGCG CCCTTATTTG TCGCCACCGC CCATCCGGTT ACCCGCAAAG 1151 CCCTCAACCG CCTGATTATG GCGCAGGATA CAGGCAGCGC GATCAAAGGC 1201 GCGGTGCGCG TGGATTATTT TTGGGGTTAC GGCGACGAAG CCGGCGAACT 1251 TGCCGGCAAA CAGAAAACCA CGGGATACGT CTGGCAGCTC CTGCCCAACG 1301 GCATGAAGCC CGAATACCGC CCGTGA This corresponds to the amino acid sequence <SEQ ID 3072; ORF 919.ng>: g919.pep 1 MKKHLLRSAL YGIAAAILAA CQSRSIQTFP QPDTSVINGP DRPAGIPDPA 51 GTTVAGGGAV YTWPHLSMP HWAAQDFAKS LQSFRLGCAN LKNRQGWQDV 101 CAQAFQTPVH SFQAKRFFER YFTPWQVAGN GSLAGTVTGY YEPVLKGDGR 151 RTERARFPIY GIPDDFISVP LPAGLRGGKN LVRIRQTGKN SGTIDNAGGT 201 HTADLSRFPI TARTTAIKGR FEGSRFLPYH TRNQINGGAL DGKAPILGYA 251 EDPVELFFMH IQGSGRLKTP SGKYIRIGYA DKNEHPYVSI GRYMADKGYL 301 KLGQTSMQGI KAYMRQNPQR LAEVLGQNPS YIFFRELAGS GNEGPVGALG 351 TPLMGEYAGA IDRHYITLGA PLFVATAHPV TRKALNRLIM AQDTGSAIKG 401 AVRVDYFWGY GDEAGELAGK QKTTGYVWQL LPNGMKPEYR P* ORF 919 shows 95.9 % identity over a 441 aa overlap with a predicted ORF (ORF 919.ng) from N. gonorrhoeae: m919/g919 10 20 30 40 50 60 m919. ep MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVIΝGPDRPVGIPDPAGTTVGGGGAV lll = l = l = = = = lllll g919 MKKHLLRSALYGIAAAILAACQSRSIQTFPQPDTSVIΝGPDRPAGIPDPAGTTVAGGGAV 10 20 30 40 50 60 70 80 90 100 110 120 m919. pep YTWPHLSLPHWAAQDFAKSLQSFRLGCAΝLKΝRQGWQDVCAQAFQTPVHSFQAKQFFER g919 YTWPHLSMPHWAAQDFAKSLQSFRLGCAΝLKΝRQGWQDVCAQAFQTPVHSFQAKRFFER 70 80 90 100 110 120 130 140 150 160 170 180 m919 . pep YFTPWQVAGΝGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA I I I I I I I M I I M M I I I I M I I I I I I I I I I = 1 1 I I 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 = 1 1 g919 YFTPWQVAGΝGSLAGTVTGYYEPVLKGDGRRTERARFPIYGIPDDFISVPLPAGLRGGKΝ 130 140 150 160 170 180 190 200 210 220 230 240 m919. pep LVRIRQTGKΝSGTIDΝTGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRΝQIΝGGAL g919 LVRIRQTGKΝSGTIDΝAGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRΝQIΝGGAL 190 200 210 220 230 240 250 260 270 280 290 300 m919.pep DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKΝEHPYVSIGRYMADKGYL g919 DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKΝEHPYVSIGRYMADKGYL 250 260 270 280 290 300 310 320 330 340 350 360 m919.pep KLGQTSMQGIKSYMRQΝPQRLAEVLGQΝPSYIFFRELAGSSΝDGPVGALGTPLMGEYAGA g919 KLGQTSMQGIKAYMRQΝPQRLAEVLGQΝPSYIFFRELAGSGΝEGPVGALGTPLMGEYAGA 310 320 330 340 350 360 370 380 390 400 410 420 m919.pep VDRHYITLGAPLFVATAHPVTRKALΝRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK = g919 IDRHYITLGAPLFVATAHPVTRKALΝRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK 370 380 390 400 410 420 430 440 m919.pep QKTTGYVWQLLPNGMKPEYRPX g919 QKTTGYVWQLLPNGMKPEYRPX 430 440 The following partial DNA sequence was identified in N meningitidis <SEQ ID 3073>: a919.seq 1 ATGAAAAAAT ACCTATTCCG CGCCGCCCTG TGCGGCATCG CCGCCGCCAT 51 CCTCGCCGCC TGCCAAAGCA AGAGCATCCA AACCTTTCCG CAACCCGACA 101 CATCCGTCAT CAACGGCCCG GACCGGCCGG TCGGCATCCC CGACCCCGCC 151 GGAACGACGG TCGGCGGCGG CGGGGCCGTT TATACCGTTG TGCCGCACCT 201 GTCCCTGCCC CACTGGGCGG CGCAGGATTT CGCCAAAAGC CTGCAATCCT 251 TCCGCCTCGG CTGCGCCAAT TTGAAAAACC GCCAAGGCTG GCAGGATGTG 301 TGCGCCCAAG CCTTTCAAAC CCCCGTCCAT TCCGTTCAGG CAAAACAGTT 351 TTTTGAACGC TATTTCACGC CGTGGCAGGT TGCAGGCAAC GGAAGCCTTG 401 CCGGTACGGT TACCGGCTAT TACGAGCCGG TGCTGAAGGG CGACGACAGG 451 CGGACGGCAC AAGCCCGCTT CCCGATTTAC GGTATTCCCG ACGATTTTAT 501 CTCCGTCCCC CTGCCTGCCG GTTTGCGGAG CGGAAAAGCC CTTGTCCGCA 551 TCAGGCAGAC GGGAAAAAAC AGCGGCACAA TCGACAATAC CGGCGGCACA 601 CATACCGCCG ACCTCTCCCA ATTCCCCATC ACTGCGCGCA CAACGGCAAT 651 CAAAGGCAGG TTTGAAGGAA GCCGCTTCCT CCCCTACCAC ACGCGCAACC 701 AAATCAACGG CGGCGCGCTT GACGGCAAAG CCCCGATACT CGGTTACGCC 751 GAAGACCCCG TCGAACTTTT TTTTATGCAC ATCCAAGGCT CGGGCCGTCT 801 GAAAACCCCG TCCGGCAAAT ACATCCGCAT CGGCTATGCC GACAAAAACG 851 AACATCCCTA CGTTTCCATC GGACGCTATA TGGCGGACAA AGGCTACCTC 901 AAGCTCGGGC AGACCTCGAT GCAGGGCATC AAAGCCTATA TGCAGCAAAA 951 CCCGCAACGC CTCGCCGAAG TTTTGGGGCA AAACCCCAGC TATATCTTTT 1001 TCCGAGAGCT TACCGGAAGC AGCAATGACG GCCCTGTCGG CGCACTGGGC 1051 ACGCCGCTGA TGGGCGAGTA CGCCGGCGCA GTCGACCGGC ACTACATTAC 1101 CTTGGGCGCG CCCTTATTTG TCGCCACCGC CCATCCGGTT ACCCGCAAAG 1151 CCCTCAACCG CCTGATTATG GCGCAGGATA CCGGCAGCGC GATTAAAGGC 1201 GCGGTGCGCG TGGATTATTT TTGGGGATAC GGCGACGAAG CCGGCGAACT 1251 TGCCGGCAAA CAGAAAACCA CGGGATATGT CTGGCAGCTT CTGCCCAACG 1301 GTATGAAGCC CGAATACCGC CCGTAA This corresponds to the amino acid sequence <SEQ ID 3074; ORF 919.a>: a919.pep 1 MKKYLFRAAL CGIAAAILAA CQSKSIQTFP QPDTSVINGP DRPVGIPDPA 51 GTTVGGGGAV YTWPHLSLP HWAAQDFAKS LQSFRLGCAN LKNRQGWQDV 101 CAQAFQTPVH SVQAKQFFER YFTPWQVAGN GSLAGTVTGY YEPVLKGDDR 151 RTAQARFPIY GIPDDFISVP LPAGLRSGKA LVRIRQTGKN SGTIDNTGGT 201 HTADLSQFPI TARTTAIKGR FEGSRFLPYH TRNQINGGAL DGKAPILGYA 251 EDPVELFFMH IQGSGRLKTP SGKYIRIGYA DKNEHPYVSI GRYMADKGYL 301 KLGQTSMQGI KAYMQQΝPQR LAEVLGQNPS YIFFRELTGS SNDGPVGALG 351 TPLMGEYAGA VDRHYITLGA PLFVATAHPV TRKALNRLIM AQDTGSAIKG 401 AVRVDYFWGY GDEAGELAGK QKTTGYVWQL LPNGMKPEYR p* m919/a919 ORFs 919 and 919.a showed a 98.6% identity in 441 aa overlap 10 20 30 40 50 60 m919.pep MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPDPAGTTVGGGGAV a919 MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPDPAGTTVGGGGAV 10 20 30 40 50 60 70 80 90 100 110 120 m919. pep YTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a919 YTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSVQAKQFFER 70 80 90 100 110 120 130 140 150 160 170 180 m919.pep YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a919 YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA 130 140 150 160 170 180 190 200 210 220 230 240 m919.pep LVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL I I I I I I I I I I I I I I I I I I II I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a919 LVRIRQTGKNSGTIDNTGGTHTADLSQFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL 190 200 210 220 230 240 250 260 270 280 290 300 m919.pep DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II a919 DGKAPILGYAEDPVELFFMHIQGSGRLKTPΞGKYIRIGYADKNEHPYVSIGRYMADKGYL 250 260 270 280 290 300 310 320 330 340 350 360 919.pep KLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFFRELAGSSNDGPVGALGTPLMGEYAGA I I I I I I I I I I I : I I : I I I I I I I I I I I I I I I I I I I I I I : I I I I I I II I I I I I I I I I I I I I I a919 KLGQTSMQGIKAYMQQNPQRLAEVLGQNPSYIFFRELTGSSNDGPVGALGTPLMGEYAGA 310 320 330 340 350 360 370 380 390 400 410 420 m919. pep VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a919 VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK 370 380 390 400 410 420 430 440 919. pep QKTTGYVWQLLPNGMKPEYRPX I I I I I I I I I I I I I I I I I I I I I I a919 QKTTGYVWQLLPNGMKPEYRPX 430 440 121 and 121-1 The following partial DNA sequence was identified in N meningitidis <SEQ ID 3075>: 1 ATGGAAACAC AGCTTTACAT CGGCATCATG TCGGGAACCA GCATGGACGG 51 GGCGGATGCC GTACTGATAC GGATGGACGG CGGCAAATGG CTGGGCGCGG 101 AAGGGCACGC CTTTACCCCC TACCCCGGCA GGTTACGCCG CCAATTGCTG 151 GATTTGCAGG ACACAGGCGC AGACGAACTG CACCGCAGCA GGATTTTGTC 201 GCAAGAACTC AGCCGCCTAT ATGCGCAAAC CGCCGCCGAA CTGCTGTGCA 251 GTCAAAACCT CGCACCGTCC GACATTACCG CCCTCGGCTG CCACGGGCAA 301 ACCGTCCGAC ACGCGCCGGA ACACGGTTAC AGCATACAGC TTGCCGATTT 351 GCCGCTGCTG GCGxxxxxxx xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx 401 xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx 451 xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx 501 xxxxxxxxxx xxxxxxxxxx xxxxxxxxx xxxxxxxxxx xxxxxxxxxx 551 xxxxxxxxxx xxxxxxxxx xxxxxxxxxx xxxxxxxxx xxxxxxxxxx 601 xxxxxxCAGC TTCCTTACGA CAAAAACGGT GCAAAGTCGG CACAAGGCAA 651 CA TTGCCG CAACTGCTCG ACAGGCTGCT CGCCCACCCG TATTTCGCAC 701 AACGCCACCC TAAAAGCACG GGGCGCGAAC TGTTTGCCAT AAATTGGCTC 751 GAAACCTACC TTGACGGCGG CGAAAACCGA TACGACGTAT TGCGGACGCT 801 TTCCCGTTTT ACCGCGCAAA CCGTTTGCGA CGCCGTCTCA CACGCAGCGG 851 CAGATGCCCG TCAAATGTAC ATTTGCGACG GCGGCATCCG CAATCCTGTT 901 TTAATGGCGG ATTTGGCAGA ATGTTTCGGC ACACGCGTTT CCCTGCACAG 951 CACCGCCGAC CTGAACCTCG ATCCGCAATG GGTGGAAGCC GCCGnATTTG 1001 CGTGGTTGGC GGCGTGTTGG ATTAATCGCA TTCCCGGTAG TCCGCACAAA 1051 GCAACCGGCG CATCCAAACC GTGTATTCTG AnCGCGGGAT ATTATTATTG 1101 A This corresponds to the amino acid sequence <SEQ ID 3076; ORF 121>: ml21.pep 1 METQLYIGIM SGTSMDGADA VLIRMDGGKW LGAEGHAFTP YPGRLRRQLL 51 DLQDTGADEL HRSRILSQEL SRLYAQTAAE LLCSQNLAPS DITALGCHGQ 101 TVRHAPEHGY SIQLADLPLL Axxxxxxxxx xxxxxxxxxx xxxxxxxxxx 151 xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx 201 xxQLPYDKNG AKSAQGNILP QLLDRLLAHP YFAQRHPKST GRELFAINWL 251 ETYLDGGENR YDVLRTLSRF TAQTVCDAVS HAAADARQMY ICDGGIRNPV 301 LMADLAECFG TRVSLHSTAD LNLDPQWVEA AXFAWLAACW INRIPGSPHK 351 ATGASKPCIL XAGYYY* The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 3077>: gl21.seq 1 ATGGAAACAC AGCTTTACAT CGGCATTATG TCGGGAACCA GTATGGACGG 51 GGCGGATGCC GTGCTGGTAC GGATGGACGG CGGCAAATGG CTGGGCGCGG 101 AAGGGCACGC CTTTACCCCC TACCCTGACC GGTTGCGCCG CAAATTGCTG 151 GATTTGCAGG ACACAGGCAC AGACGAACTG CACCGCAGCA GGATGTTGTC 201 GCAAGAACTC AGCCGCCTGT ACGCGCAAAC CGCCGCCGAA CTGCTGTGCA 251 GTCAAAACCT CGCTCCGTGC GACATTACCG CCCTCGGCTG CCACGGGCAA 301 ACCGTCCGAC ACGCGCCGGA ACACGGTtac AGCATACAGC TTGCCGATTT 351 GCCGCTGCTG GCGGAACTGa cgcggatttT TACCGTCggc gacttcCGCA 401 GCCGCGACCT TGCTGCCGGC GGacaAGGTG CGCCGCTCGT CCCCGCCTTT 451 CACGAAGCCC TGTTCCGCGA TGACAGGGAA ACACGCGTGG TACTGAACAT 501 CGGCGGGATT GCCAACATCA GCGTACTCCC CCCCGGCGCA CCCGCCTTCG 551 GCTTCGACAC AGGGCCGGGC AATATGCTGA TGGAcgcgtg gacgcaggca 601 cacTGGcagc TGCCTTACGA CAAAAacggt gcAAAGgcgg cacAAGGCAA 651 catatTGCcg CAACTGCTCG gcaggctGCT CGCCcaccCG TATTTCTCAC 701 AACCCcaccc aaAAAGCACG GGgcGCGaac TgtttgcccT AAattggctc 751 gaaacctAcc ttgacggcgg cgaaaaccga tacgacgtat tgcggacgct 801 ttcccgattc accgcgcaaA ccgTttggga cgccgtctca CACGCAGCGG 851 CAGATGCCCG TCAAATGTAC ATTTGCGGCG GCGGCATCCG CAATCCTGTT 901 TTAATGGCGG ATTTGGCAGA ATGTTTCGGC ACACGCGTTT CCCTGCACAG 951 CACCGCCGAA CTGAACCTCG ATCCTCAATG GGTGGAGGCG gccgCATTtg 1001 cgtggttggC GGCGTGTTGG ATTAACCGCA TTCCCGGTAG TCCGCACAAA 1051 GCGACCGGCG CATCCAAACC GTGTATTCTG GGCGCGGGAT ATTATTATTG 1101 A This corresponds to the amino acid sequence <SEQ ID 3078; ORF 121. ng>: gl21.pep 1 METQLYIGIM SGTSMDGADA VLVRMDGGKW LGAEGHAFTP YPDRLRRKLL 51 DLQDTGTDEL HRSRMLSQEL SRLYAQTAAE LLCSQΝLAPC DITALGCHGQ 101 TVRHAPEHGY SIQLADLPLL AELTRIFTVG DFRSRDLAAG GQGAPLVPAF 151 HEALFRDDRE TRVVLΝIGGI AΝISVLPPGA PAFGFDTGPG ΝMLMDAWTQA 201 HWQLPYDKΝG AKAAQGΝILP QLLGRLLAHP YFSQPHPKST GRELFALΝWL 251 ETYLDGGENR YDVLRTLSRF TAQTVWDAVS HAAADARQMY ICGGGIRNPV 301 LMADLAECFG TRVSLHSTAE LNLDPQWVEA AAFAWLAACW INRIPGSPHK 351 ATGASKPCIL GAGYYY* ORF 121 shows 73.5% identity over a 366 aa overlap with a predicted ORF (ORF121.ng) from N. gonorrhoeae: ml21/gl21 10 20 30 40 50 60 ml21. pep METQLYIGIMSGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPGRLRRQLLDLQDTGADEL I I I I I I I I I I I I I I I I I I I I I I: I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I : I I I gl21 METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDRLRRKLLDLQDTGTDEL 10 20 30 40 50 60 70 80 90 100 110 120 ml21.pep HRSRILSQELSRLYAQTAAELLCSQΝLAPSDITALGCHGQTVRHAPEHGYSIQLADLPLL gl21 HRSRMLSQELSRLYAQTAAELLCSQNLAPCDITALGCHGQTVRHAPEHGYSIQLADLPLL 70 80 90 100 110 120 130 140 150 160 170 180 ml21. pep AXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX I : : : gl21 AELTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDDRETRVVLNIGGIANISVLPPGA 130 140 150 160 170 180 190 200 210 220 230 240 ml21. pep XXXXXXXXXXXXXXXXXXXXXXQLPYDKNGAKSAQGNILPQLLDRLLAHPYFAQRHPKST : : I I I II I I I I I : I I I I I I I I I I I I I I I I I I : I I I I I I gl21 PAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNILPQLLGRLLAHPYFSQPHPKST 190 200 210 220 230 240 250 260 270 280 290 300 ml21. pep GRELFAINWLETYLDGGENRYDVLRTLSRFTAQTVCDAVSHAAADARQMYICDGGIRNPV I I I I I I : I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I gl21 GRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVWDAVSHAAADARQMYICGGGIRNPV 250 260 270 280 290 300 310 320 330 340 350 360 ml21. pep LMADLAECFGTRVSLHSTADLNLDPQWVEAAXFAWLAACWINRIPGSPHKATGASKPCIL I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl21 LMADLAECFGTRVSLHSTAELNLDPQWVEAAAFAWLAACWINRIPGSPHKATGASKPCIL 310 320 330 340 350 360 ml21. pep XAGYYYX I I I I I I gl21 GAGYYYX The following partial DNA sequence was identified in N meningitidis <SEQ ID 3079>: al21. seq 1 ATGGAAACAC AGCTTTACAT CGGCATCATG TCGGGAACCA GCATGGACGG 51 GGCGGATGCC GTACTGATAC GGATGGACGG CGGCAAATGG CTGGGCGCGG 101 AAGGGCACGC CTTTACCCCC TACCCCGGCA GGTTACGCCG CAAATTGCTG 151 GATTTGCAGG ACACAGGCGC GGACGAACTG CACCGCAGCA GGATGTTGTC 201 GCAAGAACTC AGCCGCCTGT ACGCGCAAAC CGCCGCCGAA CTGCTGTGCA 251 GTCAAAACCT CGCGCCGTCC GACATTACCG CCCTCGGCTG CCACGGGCAA 301 ACCGTCAGAC ACGCGCCGGA ACACAGTTAC AGCGTACAGC TTGCCGATTT 351 GCCGCTGCTG GCGGAACGGA CTCAGATTTT TACCGTCGGC GACTTCCGCA 401 GCCGCGACCT TGCGGCCGGC GGACAAGGCG CGCCGCTCGT CCCCGCCTTT 451 CACGAAGCCC TGTTCCGCGA CGACAGGGAA ACACGCGCGG TACTGAACAT 501 CGGCGGGATT GCCAACATCA GCGTACTCCC CCCCGACGCA CCCGCCTTCG 551 GCTTCGACAC AGGACCGGGC AATATGCTGA TGGACGCGTG GATGCAGGCA 601 CACTGGCAGC TTCCTTACGA CAAAAACGGT GCAAAGGCGG CACAAGGCAA 651 CATATTGCCG CAACTGCTCG ACAGGCTGCT CGCCCACCCG TATTTCGCAC 701 AACCCCACCC TAAAAGCACG GGGCGCGAAC TGTTTGCCCT AAATTGGCTC 751 GAAACCTACC TTGACGGCGG CGAAAACCGA TACGACGTAT TGCGGACGCT 801 TTCCCGATTC ACCGCGCAAA CCGTTTTCGA CGCCGTCTCA CACGCAGCGG 851 CAGATGCCCG TCAAATGTAC ATTTGCGGCG GCGGCATCCG CAATCCTGTT 901 TTAATGGCGG ATTTGGCAGA ATGTTTCGGC ACACGCGTTT CCCTGCACAG 951 CACCGCCGAA CTGAACCTCG ATCCGCAATG GGTAGAAGCC GCCGCGTTCG 1001 CATGGATGGC GGCGTGTTGG GTCAACCGCA TTCCCGGTAG TCCGCACAAA 1051 GCAACCGGCG CATCCAAACC GTGTATTCTG GGCGCGGGAT ATTATTATTG 1101 A This corresponds to the amino acid sequence <SEQ ID 3080; ORF 121. a>: al21.pep 1 METQLYIGIM SGTSMDGADA VLIRMDGGKW LGAEGHAFTP YPGRLRRKLL 51 DLQDTGADEL HRSRMLSQEL SRLYAQTAAE LLCSQΝLAPS DITALGCHGQ 101 TVRHAPEHSY SVQLADLPLL AERTQIFTVG DFRSRDLAAG GQGAPLVPAF 151 HEALFRDDRE TRAVLΝIGGI AΝISVLPPDA PAFGFDTGPG ΝMLMDAWMQA 201 HWQLPYDKΝG AKAAQGΝILP QLLDRLLAHP YFAQPHPKST GRELFALΝWL 251 ETYLDGGENR YDVLRTLSRF TAQTVFDAVS HAAADARQMY ICGGGIRNPV 301 LMADLAECFG TRVSLHSTAE LNLDPQWVEA AAFAWMAACW VNRIPGSPHK 351 ATGASKPCIL GAGYYY* ml21/al21 ORFs 121 and 121 . a showed a 74 . 0 % identity in 3 66 aa overlap 10 20 30 40 50 60 ml21. pep METQLYIGIMSGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPGRLRRQLLDLQDTGADEL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I al21 METQLYIGIMSGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPGRLRRKLLDLQDTGADEL 10 20 30 40 50 60 70 80 90 100 110 120 ml21. pep HRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTVRHAPEHGYSIQLADLPLL I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I : I I I I I I I I al21 HRSRMLSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTVRHAPEHSYSVQLADLPLL 70 80 90 100 110 120 130 140 150 160 170 180 ml21. pep AXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX I : : : al21 AERTQIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDDRETRAVLNIGGIANISVLPPDA 130 140 150 160 170 180 190 200 210 220 230 240 ml21. pep XXXXXXXXXXXXXXXXXXXXXXQLPYDKNGAKSAQGNILPQLLDRLLAHPYFAQRHPKST : I I II I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I al21 PAFGFDTGPGNMLMDAWMQAHWQLPYDKNGAKAAQGNILPQLLDRLLAHPYFAQPHPKST 190 200 210 220 230 240 250 260 270 280 290 300 ml21.pep GRELFAINWLETYLDGGENRYDVLRTLSRFTAQTVCDAVSHAAADARQMYICDGGIRNPV I I I I I I: I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I al21 GRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVFDAVSHAAADARQMYICGGGIRNPV 250 260 270 280 290 300 310 320 330 340 350 360 ml21.pep LMADLAECFGTRVSLHSTADLNLDPQWVEAAXFAWLAACWINRIPGSPHKATGASKPCIL I I I I I I I I I I I I I I I I I I I: I I I I I I I I I I I I I I : I I I I : I I I I I I I I I I I I I I I I I I I al21 LMADLAECFGTRVSLHSTAELNLDPQWVEAAAFAWMAACWVNRIPGSPHKATGASKPCIL 310 320 330 340 350 360 ml21. pep XAGYYYX I I I I I I al21 GAGYYYX Further work revealed the DNA sequence identified in N meningitidis <SEQ ID 3081>: ml21-l.seq 1 ATGGAAACAC AGCTTTACAT CGGCATCATG TCGGGAACCA GCATGGACGG 51 GGCGGATGCC GTACTGATAC GGATGGACGG CGGCAAATGG CTGGGCGCGG 101 AAGGGCACGC CTTTACCCCC TACCCCGGCA GGTTACGCCG CCAATTGCTG 151 GATTTGCAGG ACACAGGCGC AGACGAACTG CACCGCAGCA GGATTTTGTC 201 GCAAGAACTC AGCCGCCTAT ATGCGCAAAC CGCCGCCGAA CTGCTGTGCA 251 GTCAAAACCT CGCACCGTCC GACATTACCG CCCTCGGCTG CCACGGGCAA 301 ACCGTCCGAC ACGCGCCGGA ACACGGTTAC AGCATACAGC TTGCCGATTT 351 GCCGCTGCTG GCGGAACGGA CGCGGATTTT TACCGTCGGC GACTTCCGCA 401 GCCGCGACCT TGCGGCCGGC GGACAAGGCG CGCCACTCGT CCCCGCCTTT 451 CACGAAGCCC TGTTCCGCGA CAACAGGGAA ACACGCGCGG TACTGAACAT 501 CGGCGGGATT GCCAACATCA GCGTACTCCC CCCCGACGCA CCCGCCTTCG 551 GCTTCGACAC AGGGCCGGGC AATATGCTGA TGGACGCGTG GACGCAGGCA 601 CACTGGCAGC TTCCTTACGA CAAAAACGGT GCAAAGGCGG CACAAGGCAA 651 CATATTGCCG CAACTGCTCG ACAGGCTGCT CGCCCACCCG TATTTCGCAC 701 AACCCCACCC TAAAAGCACG GGGCGCGAAC TGTTTGCCCT AAATTGGCTC 751 GAAACCTACC TTGACGGCGG CGAAAACCGA TACGACGTAT TGCGGACGCT 801 TTCCCGTTTT ACCGCGCAAA CCGTTTGCGA CGCCGTCTCA CACGCAGCGG 851 CAGATGCCCG TCAAATGTAC ATTTGCGGCG GCGGCATCCG CAATCCTGTT 901 TTAATGGCGG ATTTGGCAGA ATGTTTCGGC ACACGCGTTT CCCTGCACAG 951 CACCGCCGAC CTGAACCTCG ATCCGCAATG GGTGGAAGCC GCCGNATTTG 1001 CGTGGTTGGC GGCGTGTTGG ATTAATCGCA TTCCCGGTAG TCCGCACAAA 1051 GCAACCGGCG CATCCAAACC GTGTATTCTG ANCGCGGGAT ATTATTATTG 1101 A This corresponds to the amino acid sequence <SEQ ID 3082; ORF 121-1>: ml21-l.pep 1 METQLYIGIM SGTSMDGADA VLIRMDGGKW LGAEGHAFTP YPGRLRRQLL 51 DLQDTGADEL HRSRILSQEL SRLYAQTAAE LLCSQNLAPS DITALGCHGQ 101 TVRHAPEHGY SIQLADLPLL AERTRIFTVG DFRSRDLAAG GQGAPLVPAF 151 HEALFRDNRE TRAVLNIGGI ANISVLPPDA PAFGFDTGPG NMLMDAWTQA 201 HWQLPYDKNG AKAAQGNILP QLLDRLLAHP YFAQPHPKST GRELFALNWL 251 ETYLDGGENR YDVLRTLSRF TAQTVCDAVS HAAADARQMY ICGGGIRNPV 301 LMADLAECFG TRVSLHSTAD LNLDPQWVEA AXFAWLAACW INRIPGSPHK 351 ATGASKPCIL XAGYYY* ml21-l/gl21 ORFs 121-1 and 121. ng showed a 95.6% identity in 366 aa overlap 10 20 30 40 50 60 ml21-l.pep METQLYIGIMSGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPGRLRRQLLDLQDTGADEL I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I II II I I I I I I I : I I I I I I I I : I I I gl21 METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDRLRRKLLDLQDTGTDEL 10 20 30 40 50 60 70 80 90 100 110 120 ml21-l.pep HRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTVRHAPEHGYSIQLADLPLL I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl21 HRSRMLSQELSRLYAQTAAELLCSQNLAPCDITALGCHGQTVRHAPEHGYSIQLADLPLL 70 80 90 100 110 120 130 140 150 160 170 180 ml21-l . pep AERTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDNRETRAVLNIGGIANISVLPPDA II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I : I I II I I I I I I I I I I I I gl21 AELTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDDRETRWLNIGGIANISVLPPGA 130 140 150 160 170 180 190 200 210 220 230 240 ml21-l . pep PAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNILPQLLDRLLAHPYFAQPHPKST I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I gl21 PAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNILPQLLGRLLAHPYFSQPHPKST 190 200 210 220 230 240 250 260 270 280 290 300 ml21-l . pep GRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVCDAVSHAAADARQMYICGGGIRNPV I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II gl21 GRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVWDAVSHAAADARQMYICGGGIRNPV 250 260 270 280 290 300 310 320 330 340 350 360 ml21-l.pep LMADLAECFGTRVSLHSTADLNLDPQWVEAAXFAWLAACWINRIPGSPHKATGASKPCIL I I I I I I I I I I I I II I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl21 LMADLAECFGTRVSLHSTAELNLDPQWVEAAAFAWLAACWINRIPGSPHKATGASKPCIL 310 320 330 340 350 360 ml21-l.pep XAGYYYX I I I I I I gl21 GAGYYYX The following DNA sequence was identified in N meningitidis <SEQ ID 3083>: al21-l . seq 1 ATGGAAACAC AGCTTTACAT CGGCATCATG TCGGGAACCA GCATGGACGG 51 GGCGGATGCC GTACTGATAC GGATGGACGG CGGCAAATGG CTGGGCGCGG 101 AAGGGCACGC CTTTACCCCC TACCCCGGCA GGTTACGCCG CAAATTGCTG 151 GATTTGCAGG ACACAGGCGC GGACGAACTG CACCGCAGCA GGATGTTGTC 201 GCAAGAACTC AGCCGCCTGT ACGCGCAAAC CGCCGCCGAA CTGCTGTGCA 251 GTCAAAACCT CGCGCCGTCC GACATTACCG CCCTCGGCTG CCACGGGCAA 301 ACCGTCAGAC ACGCGCCGGA ACACAGTTAC AGCGTACAGC TTGCCGATTT 351 GCCGCTGCTG GCGGAACGGA CTCAGATTTT TACCGTCGGC GACTTCCGCA 401 GCCGCGACCT TGCGGCCGGC GGACAAGGCG CGCCGCTCGT CCCCGCCTTT 451 CACGAAGCCC TGTTCCGCGA CGACAGGGAA ACACGCGCGG TACTGAACAT 501 CGGCGGGATT GCCAACATCA GCGTACTCCC CCCCGACGCA CCCGCCTTCG 551 GCTTCGACAC AGGACCGGGC AATATGCTGA TGGACGCGTG GATGCAGGCA 601 CACTGGCAGC TTCCTTACGA CAAAAACGGT GCAAAGGCGG CACAAGGCAA 651 CATATTGCCG CAACTGCTCG ACAGGCTGCT CGCCCACCCG TATTTCGCAC 701 AACCCCACCC TAAAAGCACG GGGCGCGAAC TGTTTGCCCT AAATTGGCTC 751 GAAACCTACC TTGACGGCGG CGAAAACCGA TACGACGTAT TGCGGACGCT 801 TTCCCGATTC ACCGCGCAAA CCGTTTTCGA CGCCGTCTCA CACGCAGCGG 851 CAGATGCCCG TCAAATGTAC ATTTGCGGCG GCGGCATCCG CAATCCTGTT 901 TTAATGGCGG ATTTGGCAGA ATGTTTCGGC ACACGCGTTT CCCTGCACAG 951 CACCGCCGAA CTGAACCTCG ATCCGCAATG GGTAGAAGCC GCCGCGTTCG 1001 CATGGATGGC GGCGTGTTGG GTCAACCGCA TTCCCGGTAG TCCGCACAAA 1051 GCAACCGGCG CATCCAAACC GTGTATTCTG GGCGCGGGAT ATTATTATTG 1101 A This corresponds to the amino acid sequence <SEQ ID 3084; ORF 121-1. a>: al21-l.pep 1 METQLYIGIM SGTSMDGADA VLIRMDGGKW LGAEGHAFTP YPGRLRRKLL 51 DLQDTGADEL HRSRMLSQEL SRLYAQTAAE LLCSQNLAPS DITALGCHGQ 101 TVRHAPEHSY SVQLADLPLL AERTQIFTVG DFRΞRDLAAG GQGAPLVPAF 151 HEALFRDDRE TRAVLNIGGI ANISVLPPDA PAFGFDTGPG NMLMDAWMQA 201 HWQLPYDKNG AKAAQGNILP QLLDRLLAHP YFAQPHPKST GRELFALNWL 251 ETYLDGGENR YDVLRTLSRF TAQTVFDAVS HAAADARQMY ICGGGIRNPV 301 LMADLAECFG TRVSLHSTAE LNLDPQWVEA AAFAWMAACW VNRIPGSPHK 351 ATGASKPCIL GAGYYY* ml21-l/al21-l ORFs 121-1 and 121-1. a showed a 96.4% identity in 366 aa overlap 10 20 30 40 50 60 ml21-l . pep METQLYIGIMSGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPGRLRRQLLDLQDTGADEL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I al21-l METQLYIGIMSGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPGRLRRKLLDLQDTGADEL 10 20 30 40 50 60 70 80 90 100 110 120 ml21-l.pep HRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTVRHAPEHGYSIQLADLPLL I I I I : I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I : I I : I I I I I I I I al21-l HRSRMLSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTVRHAPEHSYSVQLADLPLL 70 80 90 100 110 120 130 140 150 160 170 180 ml21-l.pep AERTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDNRETRAVLNIGGIANISVLPPDA I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I al21-l AERTQIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDDRETRAVLNIGGIANISVLPPDA 130 140 150 160 170 180 190 200 210 220 230 240 ml21-l.pep PAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNILPQLLDRLLAHPYFAQPHPKST I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al21-l PAFGFDTGPGNMLMDAWMQAHWQLPYDKNGAKAAQGNILPQLLDRLLAHPYFAQPHPKST 190 200 210 220 230 240 250 260 270 280 290 300 ml21-l . pep GRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVCDAVSHAAADARQMYICGGGIRNPV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al21-l GRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVFDAVSHAAADARQMYICGGGIRNPV 250 260 270 280 290 300 310 320 330 340 350 360 ml21-l.pep LMADLAECFGTRVSLHSTADLNLDPQWVEAAXFAWLAACWINRIPGSPHKATGASKPCIL I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I : I I II : I I I I I I I I I I I I I I I I I I I al21 LMADLAECFGTRVSLHSTAELNLDPQWVEAAAFAWMAACWVNRIPGSPHKATGASKPCIL 310 320 330 340 350 360 ml21-l.ρep XAGYYYX I I I I I I al21 GAGYYYX 128 and 128-1 The following partial DNA sequence was identified in N meningitidis <SEQ ID 3085>: ml28.seq (partial) 1 ATGACTGACA ACGCACTGCT CCATTTGGGC GAAGAACCCC GTTTTGATCA 51 AATCAAAACC GAAGACATCA AACCCGCCCT GCAAACCGCC ATCGCCGAAG 101 CGCGCGAACA AATCGCCGCC ATCAAAGCCC AAACGCACAC CGGCTGGGCA 151 AACACTGTCG AACCCCTGAC CGGCATCACC GAACGCGTCG GCAGGATTTG 201 GGGCGTGGTG TCGCACCTCA ACTGCGTCGC CGACACGCCC GAACTGCGCG 251 CCGTCTATAA CGAACTGATG CCCGAAATCA CCGTCTTCTT CACCGAAATC 301 GGACAAGACA TCGAGCTGTA CAACCGCTTC AAAACCATCA AAAATTCCCC 351 CGAATTCGAC ACCCTCTCCC CCGCACAAAA AACCAAACTC AACCAC 1 TACGCCAGCG AAAAACTGCG CGAAGCCAAA TACGCGTTCA GCGAAACCGA 51 wGTCAAAAAA TAyTTCCCyG TCGGCAAwGT ATTAAACGGA CTGTTCGCCC 101 AAmTCAAAAA ACTmTACGGC ATCGGATTTA CCGAAAAAAC yGTCCCCGTC 151 TGGCACAAAG ACGTGCGCTA TTkTGAATTG CAACAAAACG GCGAAmCCAT 201 AGGCGGCGTT TATATGGATT TGTACGCACG CGAAGGCAAA CGCGGCGGCG 251 CGTGGATGAA CGACTACAAA GGCCGCCGCC GTTTTTCAGA CGGCACGCTG 301 CAAyTGCCCA CCGCCTACCT CGTCTGCAAC TTCGCCCCAC CCGTCGGCGG 351 CAGGGAAGCC CGCyTGAGCC ACGACGAAAT CCTCATCCTC TTCCACGAAA 401 CCGGACACGG GCTGCACCAC CTGCTTACCC AAGTGGACGA ACTGGGCGTA 451 TCCGGCATCA ACGGCGTAkA ATGGGACGCG GTCGAACTGC CCAGCCAGTT 501 TATGGAAAAT TTCGTTTGGG AATACAATGT CTTGGCACAA mTGTCAGCCC 551 ACGAAGAAAC CGGcgTTCCC yTGCCGAAAG AACTCTTsGA CAAAwTGCTC 601 GCCGCCAAAA ACTTCCAAsG CGGCATGTTC yTsGTCCGGC AAwTGGAGTT 651 CGCCCTCTTT GATATGATGA TTTACAGCGA AGACGACGAA GGCCGTCTGA 701 AAAACTGGCA ACAGGTTTTA GACAGCGTGC GCAAAAAAGT CGCCGTCATC 751 CAGCCGCCCG AATACAACCG CTTCGCCTTG AGCTTCGGCC ACATCTTCGC 801 AGGCGGCTAT TCCGCAGCTn ATTACAGCTA CGCGTGGGCG GAAGTATTGA 851 GCGCGGACGC ATACGCCGCC TTTGAAGAAA GCGACGATGT CGCCGCCACA 901 GGCAAACGCT TTTGGCAGGA AATCCTCGCC GTCGGGGnAT CGCGCAGCGG 951 nGCAGAATCC TTCAAAGCCT TCCGCGGCCG CGAACCGAGC ATAGACGCAC 1001 TCTTGCGCCA CAGCGGTTTC GACAACGCGG TCTGA This corresponds to the amino acid sequence <SEQ ID 3086; ORF 128>: ml28.pep (partial) 1 MTDΝALLHLG EEPRFDQIKT EDIKPALQTA IAEAREQIAA IKAQTHTGWA 51 ΝTVEPLTGIT ERVGRIWGW SHLΝCVADTP ELRAVYΝELM PEITVFFTEI 101 GQDIELYΝRF KTIKΝSPEFD TLSPAQKTKL ΝH // 1 YASEKLREAK YAFSETXVKK YFPVGXVLΝG LFAQXKKLYG IGFTEKTVPV 51 WHKDVRYXEL QQΝGEXIGGV YMDLYAREGK RGGAWMΝDYK GRRRFSDGTL 101 QLPTAYLVCΝ FAPPVGGREA RLSHDEILIL FHETGHGLHH LLTQVDELGV 151 SGIΝGVXWDA VELPSQFMEΝ FVWEYΝVLAQ XSAHEETGVP LPKELXDKXL 201 AAKΝFQXGMF XVRQXEFALF DMMIYSEDDE GRLKΝWQQVL DSVRKKVAVI 251 QPPEYΝRFAL SFGHIFAGGY SAAXYSYAWA EVLSADAYAA FEESDDVAAT 301 GKRFWQEILA VGXSRSGAES FKAFRGREPS IDALLRHSGF DΝAV* The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 3087>: gl28.seq 1 atgattgaca acgCActgct ccacttgggc gaagaaccCC GTTTTaatca 51 aatccaaacc gaagACAtca AACCCGCCGT CCAAACCGCC ATCGCCGAAG 101 CGCGCGGACA AATCGCCGCC GTCAAAGCGC AAACGCACAC CGGCTGGGCG 151 AACACCGTCG AGCGTCTGAC CGGCATCACC GAACGCGTCG GCAGGATTTG 201 GGGCGTCGTG TCCCATCTCA ACTCCGTCGT CGACACGCCC GAACTGCGCG 251 CCGTCTATAA CGAACTGATG CCTGAAATCA CCGTCTTCTT CACCGAAATC 301 GGACAAGACA TCGAACTGTA CAACCGCTTC AAAACCATCA AAAATTCCCC 351 CGAATTTGCA ACGCTTTCCC CCGCACAAAA AACCAAGCTC GATCACGACC 401 TGCGCGATTT CGTATTGAGC GGCGCGGAAC TGCCGCCCGA ACGGCAGGCA 451 GAACTGGCAA AACTGCAAAC CGAAGGCGCG CAACTTTCCG CCAAATTCTC 501 CCAAAACGTC CTAGACGCGA CCGACGCGTT CGGCATTTAC TTTGACGATG 551 CCGCACCGCT TGCCGGCATT CCCGAAGACG CGCTCGCCAT GTTTGCCGCC 601 GCCGCGCAAA GCGAAGGCAA AACAGGTTAC AAAATCGGCT TGCAGATTCC 651 GCACTACCTT GCCGTTATCC AATACGCCGG CAACCGCGAA CTGCGCGAAC 701 AAATCTACCG CGCCTACGTT ACCCGTGCCA GCGAACTTTC AAACGACGGC 751 AAATTCGACA ACACCGCCAA CATCGACCGC ACGCTCGAAA ACGCATTGAA 801 AACCGccaaa cTGCTCGGCT TTAAAAATTA CGCCGAATTG TCGCTGGCAA 851 CCAAAATGGC GGACACGCCC GAACAGGTTT TAAACTTCCT GCACGACCTC 901 GCCCGCCGCG CCAAACCCTA CGCCGAAAAA GACCTCGCCG AAGTCAAAGC 951 CTTCGCCCGC GAACACCTCG GTCTCGCCGA CCCGCAGCCG TGGGACTTGA 1001 GCTACGCCGG CGAAAAACTG CGCGAAGCCA AATACGCATT CAGCGAAACC 1051 GAAGTCAAAA AATACTTCCC CGTCGGCAAA GTTCTGGCAG GCCTGTTCGC 1101 CCAAATCAAA AAACTCTACG GCATCGGATT CGCCGAAAAA ACCGTTCCCG 1151 TCTGGCACAA AGACGTGCGC TATTTTGAAT TGCAACAAAA CGGCAAAACC 1 01 ATCGGCGGCG TTTATATGGA TTTGTACGCA CGCGAAGGCA AACGCGGCGG 1251 CGCGTGGATG AACGACtaca AAGGCCGCCG CCGCTTTGCC GACGgcacGC 1301 TGCAACTGCC CACCGCCTAC CTCGTCTGCA ACTTCGCCCC GCCCGTCGGC 1351 GGCAAAGAAG CGCGTTTAAG CCACGACGAA ATCCTCACCC TCTTCCACGA 1401 AacCGGCCAC GGACTGCACC ACCTGCTTAC CCAAGTGGAC GAACTGGGCG 1451 TGTCCGGCAT CAAcggcgtA GAATGGGACG CGGTCGAACT GCCCAGCCAG 1501 TTTATGGAAA ACTTCGTTTG GGAATACAAT GTATTGGCAC AAATGTCCGC 1551 CCACGAAGAA AccgGCGAGC CCCTGCCGAA AGAACTCTTC GACAAAATGC 1601 TcgcCGCCAA AAACTTCCAG CGCGGTATGT TCCTCGTCCG GCAAATGGAG 1651 TTCGCCCTCT TCGATATGAT GATTTACAGT GAAAGCGACG AATGCCGTCT 1701 GAAAAACTGG CAGCAGGTTT TAGACAGCGT GCGCAAAGAA GTcGCCGTCA 1751 TCCAACCGCC CGAATACAAC CGCTTCGCCA ACAGCTTCGG CCacatctTC 1801 GCcggcGGCT ATTCCGCAGG CTATTACAGC TACGCATGGG CCGAAGTCCt 1851 cAGCACCGAT GCCTACGCCG CCTTTGAAGA AAGcGACGac gtcGCCGCCA 1901 CAGGCAAACG CTTCTGGCAA GAAAtccttg ccgtcggcgg ctCCCGCAGC 1951 gcgGCGGAAT CCTTCAAAGC CTTCCGCGGA CGCGAACCGA GCATAGACGC 2001 ACTGCTGCGC CAaagcggtT TCGACAACGC gGCttgA This corresponds to the amino acid sequence <SEQ ID 3088; ORF 128.ng>: gl28.pep 1 MIDΝALLHLG EEPRFΝQIQT EDIKPAVQTA IAEARGQIAA VKAQTHTGWA 51 ΝTVERLTGIT ERVGRIWGW SHLΝSWDTP ELRAVYΝELM PEITVFFTEI 101 GQDIELYΝRF KTIKΝSPEFA TLSPAQKTKL DHDLRDFVLS GAELPPERQA 151 ELAKLQTEGA QLSAKFSQΝV LDATDAFGIY FDDAAPLAGI PEDALAMFAA . 201 AAQSEGKTGY KIGLQIPHYL AVIQYAGΝRE LREQIYRAYV TRASELSΝDG 251 KFDΝTAΝIDR TLEΝALKTAK LLGFKΝYAEL SLATKMADTP EQVLΝFLHDL 301 ARRAKPYAEK DLAEVKAFAR EHLGLADPQP WDLSYAGEKL REAKYAFSET 351 EVKKYFPVGK VLAGLFAQIK KLYGIGFAEK TVPVWHKDVR YFELQQΝGKT 401 IGGVYMDLYA REGKRGGAWM ΝDYKGRRRFA DGTLQLPTAY LVCΝFAPPVG 451 GKEARLSHDE I TLFHETGH GLHHLLTQVD ELGVSGIΝGV EWDAVELPSQ 501 FMEΝFVWEYΝ VLAQMSAHEE TGEPLPKELF DKMLAAKΝFQ RGMFLVRQME 551 FALFDMMIYS ESDECRLKΝW QQVLDSVRKE VAVIQPPEYΝ RFAΝSFGHIF 601 AGGYSAGYYS YAWAEVLSTD AYAAFEESDD VAATGKRFWQ EILAVGGSRS 651 AAESFKAFRG REPSIDALLR QSGFDNAA* ORF 128 shows 91.7% identity over a 475 aa overlap with a predicted ORF (ORF 128.ng) from N gonorrhoeae: ml28/gl28 10 20 30 40 50 60 gl28.pep MIDΝALLHLGEEPRFΝQIQTEDIKPAVQTAIAEARGQIAAVKAQTHTGWAΝTVERLTGIT I = l l = M I I I I I = I I M M M I I I I = I I I 1 1 1 1 1 1 1 1 1 1 M i l l ml28 MTDΝALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQTHTGWAΝTVEPLTGIT 10 20 30 40 50 60 70 80 90 100 110 120 gl28 -pep ERVGRIWGWSHLΝSWDTPELRAVYΝELMPEITVFFTEIGQDIELYΝRFKTIKΝSPEFA I = 1111 II 11111111 II 11111111111111111111111111 ml28 ERVGRIWGWSHLΝCVADTPELRAVYΝELMPEITVFFTEIGQDIELYΝRFKTIKΝSPEFD 70 80 90 100 110 120 130 140 150 160 170 180 gl28.pep TLSPAQKTKLDHDLRDFVLSGAELPPERQAELAKLQTEGAQLSAKFSQΝVLDATDAFGIY ml28 TLSPAQKTKLΝH 130 // 340 350 360 gl28 . pep YAGEKLREAKYAFSETEVKKYFPVGKVLAG I I • I I I I I I I I I I I I I I I I I I I I I I I I ml28 YASEKLREAKYAFSETXVKKYFPVGXVLΝG 10 20 30 370 380 390 400 410 420 gl28.pep LFAQIKKLYGIGFAEKTVPVWHKDVRYFELQQΝGKTIGGVYMDLYAREGKRGGAWMΝDYK MM 11 i 11111 = 11111111 !! 111 II II II = = II I II II II II 11 II 11 II I II II ml28 LFAQXKKLYGIGFTEKTVPVWHKDVRYXELQQΝGEXIGGVYMDLYAREGKRGGAWMΝDYK 40 50 60 70 80 90 430 440 450 460 470 480 gl28.pep GRRRFADGTLQLPTAYLVCΝFAPPVGGKEARLSHDEILTLFHETGHGLHHLLTQVDELGV I M 11 H I !! M 111111 i I II I II 11 = M 11111111 I II 1111 ! 1111111111111 ml28 GRRRFSDGTLQLPTAYLVCΝFAPPVGGREARLSHDEILILFHETGHGLHHLLTQVDELGV 100 110 120 130 140 150 490 500 510 520 530 540 gl28.pep SGIΝGVEWDAVELPSQFMEΝFVWEYΝVLAQMSAHEETGEPLPKELFDKMLAAKΝFQRGMF I 11111 II 111111111111111 II IIIIIM III ml28 SGIΝGVXWDAVELPSQFMEΝFVWEYΝVLAQXSAHEETGVPLPKELXDKXLAAKΝFQXGMF 160 170 180 190 200 210 550 560 570 580 590 600 gl28.pep LVRQMEFALFDMMIYSESDECRLKΝWQQVLDSVRKEVAVIQPPEYΝRFAΝSFGHIFAGGY M l I M M M M I I h l l I I I I I I I I I I I I I I = I I I I I I I I I 1 1 I I 1 1 i 1 1 1 i 1 1 ! ml28 XVRQXEFALFDMMIYSEDDEGRLKΝWQQVLDSVRKKVAVIQPPEYΝRFALSFGHIFAGGY 220 230 240 250 260 270 610 620 630 640 650 660 gl28.pep SAGYYSYAWAEVLSTDAYAAFEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPS 1 1 = 1 1 h 1 1 1 1 1 1 1 1 1 1 1 1 1 ml28 SAAXYSYAWAEVLSADAYAAFEESDDVAATGKRFWQEILAVGXSRSGAESFKAFRGREPS 280 290 300 310 320 330 670 679 gl28 . pep IDALLRQSGFDNAAX 111111=111111= ml28 IDALLRHSGFDNAVX 340 The following partial DNA sequence was identified in N meningitidis <SEQ ID 3089>: al28.seq 1 ATGACTGACA ACGCACTGCT CCATTTGGGC GAAGAACCCC GTTTTGATCA 51 AATCAAAACC GAAGACATCA AACCCGCCCT GCAAACCGCC ATTGCCGAAG 101 CGCGCGAACA AATCGCCGCC ATCAAAGCCC AAACGCACAC CGGCTGGGCA 151 AACACTGTCG AACCCCTGAC CGGCATCACC GAACGCGTCG GCAGGATTTG 201 GGGCGTGGTG TCGCACCTCA ACTCCGTCAC CGACACGCCC GAACTGCGCG 251 CCGCCTACAA TGAATTAATG CCCGAAATTA CCGTCTTCTT CACCGAAATC 301 GGACAAGACA TCGAGCTGTA CAACCGCTTC AAAACCATCA AAAACTCCCC 351 CGAGTTCGAC ACCCTCTCCC ACGCGCAAAA AACCAAACTC AACCACGATC 401 TGCGCGATTT CGTCCTCAGC GGCGCGGAAC TGCCGCCCGA ACAGCAGGCA 451 GAATTGGCAA AACTGCAAAC CGAAGGCGCG CAACTTTCCG CCAAATTCTC 501 CCAAAACGTC CTAGACGCGA CCGACGCGTT CGGCATTTAC TTTGACGATG 551 CCGCACCGCT TGCCGGCATT CCCGAAGACG CGCTCGCCAT GTTTGCCGCT 601 GCCGCGCAAA GCGAAGGCAA AACAGGCTAC AAAATCGGTT TGCAGATTCC 651 GCACTACCTC GCCGTCATCC AATACGCCGA CAACCGCAAA CTGCGCGAAC 701 AAATCTACCG CGCCTACGTT ACCCGCGCCA GCGAGCTTTC AGACGACGGC 751 AAATTCGACA ACACCGCCAA CATCGACCGC ACGCTCGAAA ACGCCCTGCA 801 AACCGCCAAA CTGCTCGGCT TCAAAAACTA CGCCGAATTG TCGCTGGCAA 851 CCAAAATGGC GGACACCCCC GAACAAGTTT TAAACTTCCT GCACGACCTC 901 GCCCGCCGCG CCAAACCCTA CGCCGAAAAA GACCTCGCCG AAGTCAAAGC 951 CTTCGCCCGC GAAAGGCTCG GCCTCGCCGA TTTGCAACCG TGGGACTTGG 1001 GCTACGCCGG CGAAAAACTG CGCGAAGCCA AATACGCATT CAGCGAAACC 1051 GAAGTCAAAA AATACTTCCC CGTCGGCAAA GTATTAAACG GACTGTTCGC 1101 CCAAATCAAA AAACTCTACG GCATCGGATT TACCGAAAAA ACCGTCCCCG 1151 TCTGGCACAA AGACGTGCGC TATTTTGAAT TGCAACAAAA CGGCGAAACC 1201 ATAGGCGGCG TTTATATGGA TTTGTACGCA CGCGAAGGCA AACGCGGCGG 1251 CGCGTGGATG AACGACTACA AAGGCCGCCG CCGTTTTTCA GACGGCACGC 1301 TGCAACTGCC CACCGCCTAC CTCGTCTGCA ACTTCACCCC GCCCGTCGGC 1351 GGCAAAGAAG CCCGCTTGAG CCATGACGAA ATCCTCACCC TCTTCCACGA 1401 AACCGGACAC GGCCTGCACC ACCTGCTTAC CCAAGTCGAC GAACTGGGCG 1451 TATCCGGCAT CAACGGCGTA GAATGGGACG CAGTCGAACT GCCCAGTCAG 1501 TTTATGGAAA ATTTCGTTTG GGAATACAAT GTCTTGGCGC AAATGTCCGC 1551 CCACGAAGAA ACCGGCGTTC CCCTGCCGAA AGAACTCTTC GACAAAATGC 1601 TCGCCGCCAA AAACTTCCAA CGCGGAATGT TCCTCGTCCG CCAAATGGAG 1651 TTCGCCCTCT TTGATATGAT GATTTACAGC GAAGACGACG AAGGCCGTCT 1701 GAAAAACTGG CAACAGGTTT TAGACAGCGT GCGCAAAGAA GTCGCCGTCG 1751 TCCGACCGCC CGAATACAAC CGCTTCGCCA ACAGCTTCGG CCACATCTTC 1801 GCAGGCGGCT ATTCCGCAGG CTATTACAGC TACGCGTGGG CGGAAGTATT 1851 GAGCGCGGAC GCATACGCCG CCTTTGAAGA AAGCGACGAT GTCGCCGCCA 1901 CAGGCAAACG CTTTTGGCAG GAAATCCTCG CCGTCGGCGG ATCGCGCAGC 1951 GCGGCAGAAT CCTTCAAAGC CTTCCGCGGA CGCGAACCGA GCATAGACGC 2001 ACTCTTGCGC CACAGCGGCT TCGACAACGC GGCTTGA This corresponds to the amino acid sequence <SEQ ID 3090; ORF 128.a>: al28.pep 1 MTDΝALLHLG EEPRFDQIKT EDIKPALQTA IAEAREQIAA IKAQTHTGWA 51 ΝTVEPLTGIT ERVGRIWGW SHLΝSVTDTP ELRAAYΝELM PEITVFFTEI 101 GQDIELYΝRF KTIKΝSPEFD TLSHAQKTKL ΝHDLRDFVLS GAELPPEQQA 151 ELAKLQTEGA QLSAKFSQΝV LDATDAFGIY FDDAAPLAGI PEDALAMFAA 201 AAQSEGKTGY KIGLQIPHYL AVIQYADΝRK LREQIYRAYV TRASELSDDG 251 KFDΝTAΝIDR TLEΝALQTAK LLGFKΝYAEL SLATKMADTP EQVLΝFLHDL 301 ARRAKPYAEK DLAEVKAFAR ESLGLADLQP WDLGYAGEKL REAKYAFSET 351 EVKKYFPVGK VLΝGLFAQIK KLYGIGFTEK TVPVWHKDVR YFELQQΝGET 401 IGGVYMDLYA REGKRGGAWM ΝDYKGRRRFS DGTLQLPTAY LVCΝFTPPVG 451 GKEARLSHDE ILTLFHETGH GLHHLLTQVD ELGVSGIΝGV EWDAVELPSQ 501 FMEΝFVWEYΝ VLAQMSAHEE TGVPLPKELF DKMLAAKΝFQ RGMFLVRQME 551 FALFDMMIYS EDDEGRLKNW QQVLDSVRKE VAWRPPEYN RFANSFGHIF 601 AGGYSAGYYS YAWAEVLΞAD AYAAFEESDD VAATGKRFWQ EILAVGGSRS 651 AAESFKAFRG REPSIDALLR HSGFDNAA* /al28 ORFs 128 and 128.a showed a 66.0% identity in 677 aa overlap 10 20 30 40 50 60 ml28. pep MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQTHTGWANTVEPLTGIT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al28 MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQTHTGWANTVEPLTGIT 10 20 30 40 50 60 70 80 90 100 110 120 ml28. pep ERVGRIWGVVSHLNCVADTPELRAVYNELMPEITVFFTEIGQDIELYNRFKTIKNSPEFD I I I I I I I I I I I I I I I : I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I l28 ERVGRIWGWSHLNSVTDTPELRAAYNELMPEITVFFTEIGQDIELYNRFKTIKNSPEFD 70 80 90 100 110 120 130 ml28 . pep TLSPAQKTKLNH I I I I I I I I I I I al28 TLSHAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGAQLSAKFSQNVLDATDAFGIY 130 140 150 160 170 180 ml 28 . pep a 128 FDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPHYLAVIQYADNRKLREQIYRAYV 190 200 210 220 230 240 ml28.pep al28 TRASELSDDGKFDNTANIDRTLENALQTAKLLGFKNYAELSLATKMADTPEQVLNFLHDL 250 260 270 280 290 300 140 150 ml28. pep YASEKLREAKYAFSETXVKKYFPVGX 11:1111111111111 I I I I I I I I al28 ARRAKPYAEKDLAEVKAFARESLGLADLQPWDLGYAGEKLREAKYAFSETEVKKYFPVGK 310 320 330 340 350 360 160 170 180 190 200 210 ml28. pep VLNGLFAQXKKLYGIGFTEKTVPVWHKDVRYXELQQNGEXIGGVYMDLYAREGKRGGAWM I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I al28 VLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYFELQQNGETIGGVYMDLYAREGKRGGAWM 370 380 390 400 410 420 220 230 240 250 260 270 ml28. pep NDYKGRRRFSDGTLQLPTAYLVCNFAPPVGGREARLSHDEILILFHETGHGLHHLLTQVD I I I I I I I I I I I I I I I I I I I I I I I I I : I I I II : I I I I I I I I I I I II I I I I I I I I I I I I I I al28 NDYKGRRRFSDGTLQLPTAYLVCNFTPPVGGKEARLSHDEILTLFHETGHGLHHLLTQVD 430 440 450 460 470 480 280 290 300 310 320 330 ml28. pep ELGVSGINGVXWDAVELPSQFMENFVWEYNVLAQXSAHEETGVPLPKELXDKXLAAKNFQ I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I II al28 ELGVSGINGVEWDAVELPSQFMENFVWEYNVLAQMSAHEETGVPLPKELFDKMLAAKNFQ 490 500 510 520 530 540 340 350 360 370 380 390 ml28.pep XGMFXVRQXEFALFDMMIYSEDDEGRLKNWQQVLDSVRKKVAVIQPPEYNRFALSFGHIF III III I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I II :: I I I I I I I I llllll al28 RGMFLVRQMEFALFDMMIYSEDDEGRLKNWQQVLDSVRKEVAWRPPEYNRFANSFGHIF 550 560 570 580 590 600 400 410 420 430 440 450 ml28. pep AGGYSAAXYSYAWAEVLSADAYAAFEESDDVAATGKRFWQEILAVGXSRSGAESFKAFRG llllll: I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I 111:111111111 al28 AGGYSAGYYSYAWAEVLSADAYAAFEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRG 610 620 630 640 650 660 460 470 ml28.pep REPSIDALLRHSGFDNAVX I I I I I I I I I I I I I I I I I : al28 REPSIDALLRHSGFDNAAX 670 Further work revealed the DNA sequence identified in N. meningitidis <SEQ ID 3091>: ml28-l.seq 1 ATGACTGACA ACGCACTGCT CCATTTGGGC GAAGAACCCC GTTTTGATCA 51 AATCAAAACC GAAGACATCA AACCCGCCCT GCAAACCGCC ATCGCCGAAG 101 CGCGCGAACA AATCGCCGCC ATCAAAGCCC AAACGCACAC CGGCTGGGCA 151 AACACTGTCG AACCCCTGAC CGGCATCACC GAACGCGTCG GCAGGATTTG 201 GGGCGTGGTG TCGCACCTCA ACTCCGTCGC CGACACGCCC GAACTGCGCG 251 CCGTCTATAA CGAACTGATG CCCGAAATCA CCGTCTTCTT CACCGAAATC 301 GGACAAGACA TCGAGCTGTA CAACCGCTTC AAAACCATCA AAAATTCCCC 351 CGAATTCGAC ACCCTCTCCC CCGCACAAAA AACCAAACTC AACCACGATC 401 TGCGCGATTT CGTCCTCAGC GGCGCGGAAC TGCCGCCCGA ACAGCAGGCA 451 GAACTGGCAA AACTGCAAAC CGAAGGCGCG CAACTTTCCG CCAAATTCTC 501 CCAAAACGTC CTAGACGCGA CCGACGCGTT CGGCATTTAC TTTGACGATG 551 CCGCACCGCT TGCCGGCATT CCCGAAGACG CGCTCGCCAT GTTTGCCGCC 601 GCCGCGCAAA GCGAAAGCAA AACAGGCTAC AAAATCGGCT TGCAGATTCC 651 ACACTACCTC GCCGTCATCC AATACGCCGA CAACCGCGAA CTGCGCGAAC 701 AAATCTACCG CGCCTACGTT ACCCGCGCCA GCGAACTTTC AGACGACGGC 751 AAATTCGACA ACACCGCCAA CATCGACCGC ACGCTCGCAA ACGCCCTGCA 801 AACCGCCAAA CTGCTCGGCT TCAAAAACTA CGCCGAATTG TCGCTGGCAA 851 CCAAAATGGC GGACACGCCC GAACAAGTTT TAAACTTCCT GCACGACCTC 901 GCCCGCCGCG CCAAACCCTA CGCCGAAAAA GACCTCGCCG AAGTCAAAGC 951 CTTCGCCCGC GAAAGCCTGA ACCTCGCCGA TTTGCAACCG TGGGACTTGG 1001 GCTACGCCAG CGAAAAACTG CGCGAAGCCA AATACGCGTT CAGCGAAACC 1051 GAAGTCAAAA AATACTTCCC CGTCGGCAAA GTATTAAACG GACTGTTCGC 1101 CCAAATCAAA AAACTCTACG GCATCGGATT TACCGAAAAA ACCGTCCCCG 1151 TCTGGCACAA AGACGTGCGC TATTTTGAAT TGCAACAAAA CGGCGAAACC 1201 ATAGGCGGCG TTTATATGGA TTTGTACGCA CGCGAAGGCA AACGCGGCGG 1251 CGCGTGGATG AACGACTACA AAGGCCGCCG CCGTTTTTCA GACGGCACGC 1301 TGCAACTGCC CACCGCCTAC CTCGTCTGCA ACTTCGCCCC ACCCGTCGGC 1351 GGCAGGGAAG CCCGCCTGAG CCACGACGAA ATCCTCATCC TCTTCCACGA 1401 AACCGGACAC GGGCTGCACC ACCTGCTTAC CCAAGTGGAC GAACTGGGCG 1451 TATCCGGCAT CAACGGCGTA GAATGGGACG CGGTCGAACT GCCCAGCCAG 1501 TTTATGGAAA ATTTCGTTTG GGAATACAAT GTCTTGGCAC AAATGTCAGC 1551 CCACGAAGAA ACCGGCGTTC CCCTGCCGAA AGAACTCTTC GACAAAATGC 1601 TCGCCGCCAA AAACTTCCAA CGCGGCATGT TCCTCGTCCG GCAAATGGAG 1651 TTCGCCCTCT TTGATATGAT GATTTACAGC GAAGACGACG AAGGCCGTCT 1701 GAAAAACTGG CAACAGGTTT TAGACAGCGT GCGCAAAAAA GTCGCCGTCA 1751 TCCAGCCGCC CGAATACAAC CGCTTCGCCT TGAGCTTCGG CCACATCTTC 1801 GCAGGCGGCT ATTCCGCAGG CTATTACAGC TACGCGTGGG CGGAAGTATT 1851 GAGCGCGGAC GCATACGCCG CCTTTGAAGA AAGCGACGAT GTCGCCGCCA 1901 CAGGCAAACG CTTTTGGCAG GAAATCCTCG CCGTCGGCGG ATCGCGCAGC 1951 GCGGCAGAAT CCTTCAAAGC CTTCCGCGGC CGCGAACCGA GCATAGACGC 2001 ACTCTTGCGC CACAGCGGTT TCGACAACGC GGTCTGA This corresponds to the amino acid sequence <SEQ ID 3092; ORF 128-1>: ml28-l.pep. 1 MTDΝALLHLG EEPRFDQIKT EDIKPALQTA IAEAREQIAA IKAQTHTGWA 51 ΝTVEPLTGIT ERVGRIWGW SHLΝSVADTP ELRAVYΝELM PEITVFFTEI 101 GQDIELYNRF KTIKNSPEFD TLSPAQKTKL NHDLRDFVLS GAELPPEQQA 151 ELAKLQTEGA QLSAKFSQNV LDATDAFGIY FDDAAPLAGI PEDALAMFAA 201 AAQSESKTGY KIGLQIPHYL AVIQYADNRE LREQIYRAYV TRASELSDDG 251 KFDNTANIDR TLANALQTAK LLGFKNYAEL SLATKMADTP EQVLNFLHDL 301 ARRAKPYAEK DLAEVKAFAR ESLNLADLQP WDLGYASEKL REAKYAFSET 351 EVKKYFPVGK VLNGLFAQIK KLYGIGFTEK TVPVWHKDVR YFELQQNGET 401 IGGVYMDLYA REGKRGGAWM NDYKGRRRFS DGTLQLPTAY LVCNFAPPVG 451 GREARLSHDE ILILFHETGH GLHHLLTQVD ELGVSGINGV EWDAVELPSQ 501 FMENFVWEYN VLAQMSAHEE TGVPLPKELF DKMLAAKNFQ RGMFLVRQME 551 FALFDMMIYS EDDEGRLKNW QQVLDSVRKK VAVIQPPEYN RFALSFGHIF 601 AGGYSAGYYS YAWAEVLSAD AYAAFEESDD VAATGKRFWQ EILAVGGSRS 651 AAESFKAFRG REPSIDALLR HSGFDNAV* The following DNA sequence was identified in N gonorrhoeae <SEQ ID 3093>: gl28-l.seq (partial) 1 ATGATTGACA ACGCACTGCT CCACTTGGGC GAAGAACCCC GTTTTAATCA 51 AATCAAAACC GAAGACATCA AACCCGCCGT CCAAACCGCC ATCGCCGAAG 101 CGCGCGGACA AATCGCCGCC GTCAAAGCGC AAACGCACAC CGGCTGGGCG 151 AACACCGTCG AGCGTCTGAC CGGCATCACC GAACGCGTCG GCAGGATTTG 201 GGGCGTCGTG TCCCATCTCA ACTCCGTCGT CGACACGCCC GAACTGCGCG 251 CCGTCTATAA CGAACTGATG CCTGAAATCA CCGTCTTCTT CACCGAAATC 301 GGACAAGACA TCGAACTGTA CAACCGCTTC AAAACCATCA AAAATTCCCC 351 CGAATTTGCA ACGCTTTCCC CCGCACAAAA AACCAAGCTC GATCACGACC 401 TGCGCGATTT CGTATTGAGC GGCGCGGAAC TGCCGCCCGA ACGGCAGGCA 451 GAACTGGCAA AACTGCAAAC CGAAGGCGCG CAACTTTCCG CCAAATTCTC 501 CCAAAACGTC CTAGACGCGA CCGACGCGTT CGGCATTTAC TTTGACGATG 551 CCGCACCGCT TGCCGGCATT CCCGAAGACG CGCTCGCCAT GTTTGCCGCC 601 GCCGCGCAAA GCGAAGGCAA AACAGGTTAC AAAATCGGCT TGCAGATTCC 651 GCACTACCTT GCCGTTATCC AATACGCCGG CAACCGCGAA CTGCGCGAAC 701 AAATCTACCG CGCCTACGTT ACCCGTGCCA GCGAACTTTC AAACGACGGC 751 AAATTCGACA ACACCGCCAA CATCGACCGC ACGCTCGAAA ACGCATTGAA 801 AACCGCCAAA CTGCTCGGCT TTAAAAATTA CGCCGAATTG TCGCTGGCAA 851 CCAAAATGGC GGACACGCCC GAACAGGTTT TAAACTTCCT GCACGACCTC 901 GCCCGCCGCG CCAAACCCTA CGCCGAAAAA GACCTCGCCG AAGTCAAAGC 951 CTTCGCCCGC GAACACCTCG GTCTCGCCGA CCCGCAGCCG TGGGACTTGA 1001 GCTACGCCGG CGAAAAACTG CGCGAAGCCA AATACGCATT CAGCGAAACC 1051 GAAGTCAAAA AATACTTCCC CGTCGGCAAA GTTCTGGCAG GCCTGTTCGC 1101 CCAAATCAAA AAACTCTACG GCATCGGATT CGCCGAAAAA ACCGTTCCCG 1151 TCTGGCACAA AGACGTGCGC TATTTTGAAT TGCAACAAAA CGGCAAAACC 1201 ATCGGCGGCG TTTATATGGA TTTGTACGCA CGCGAAGGCA AACGCGGCGG 1251 CGCGTGGATG AACGACTACA AAGGCCGCCG CCGCTTTGCC GACGGCACGC 1301 • TGCAACTGCC CACCGCCTAC CTCGTCTGCA ACTTCGCCCC GCCCGTCGGC 1351 GGCAAAGAAG CGCGTTTAAG CCACGACGAA ATCCTCACCC TCTTCCACGA 1401 AACCGGCCAC GGACTGCACC ACCTGCTTAC CCAAGTGGAC GAACTGGGCG 1451 TGTCCGGCAT CAACGGCGTA AAA This corresponds to the amino acid sequence <SEQ LD 3094; ORF 128-1. ng>: gl28-l.pep (partial) 1 MIDΝALLHLG EEPRFΝQIKT EDIKPAVQTA IAEARGQIAA VKAQTHTGWA 51 ΝTVERLTGIT ERVGRIWGW SHLΝSWDTP ELRAVYΝELM PEITVFFTEI 101 GQDIELYΝRF KTIKΝSPEFA TLSPAQKTKL DHDLRDFVLS GAELPPERQA 151 ELAKLQTEGA QLSAKFSQΝV LDATDAFGIY FDDAAPLAGI PEDALAMFAA 201 AAQSEGKTGY KIGLQIPHYL AVIQYAGΝRE LREQIYRAYV TRASELSΝDG 251 KFDΝTAΝIDR TLEΝALKTAK LLGFKΝYAEL SLATKMADTP EQVLΝFLHDL 301 ARRAKPYAEK DLAEVKAFAR EHLGLADPQP WDLSYAGEKL REAKYAFSET 351 EVKKYFPVGK VLAGLFAQIK KLYGIGFAEK TVPVWHKDVR YFELQQΝGKT 401 IGGVYMDLYA REGKRGGAWM ΝDYKGRRRFA DGTLQLPTAY LVCΝFAPPVG 451 GKEARLSHDE ILTLFHETGH GLHHLLTQVD ELGVSGIΝGV K ml28-l/gl28-l ORFs 128-1 and 128-1. ng showed a 94.5% identity in 491 aa overlap 10 20 30 40 50 60 gl28-l . pep MIDNALLHLGEEPRFNQIKTEDIKPAVQTAIAEARGQIAAVKAQTHTGWANTVERLTGIT I I I I I I I I I I I I I I : I I I I I I I I I I : I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I ml28-l MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQTHTGWANTVEPLTGIT 10 20 30 40 50 60 70 80 90 100 110 120 gl28-l . pep ERVGRIWGWSHLNSWDTPELRAVYNELMPEITVFFTEIGQDIELYNRFKTIKNSPEFA I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml28-l ERVGRIWGWSHLNSVADTPELRAVYNELMPEITVFFTEIGQDIELYNRFKTIKNSPEFD 70 80 90 100 110 120 130 140 150 160 170 180 gl28-l . pep TLSPAQKTKLDHDLRDFVLSGAELPPERQAELAKLQTEGAQLSAKFSQNVLDATDAFGIY I I I I I I I I I I : I I I I I I I I I I I I I I I I: I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml28-l TLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGAQLSAKFSQNVLDATDAFGIY 130 140 150 160 170 180 190 200 210 220 230 240 gl28-l . pep FDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPHYLAVIQYAGNRELREQIYRAYV I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml28-l FDDAAPLAGIPEDALAMFAAAAQSESKTGYKIGLQIPHYLAVIQYADNRELREQIYRAYV 190 200 210 220 230 240 250 260 270 280 290 300 gl28-l.pep TRASELSNDGKFDNTANIDRTLENALKTAKLLGFKNYAELSLATKMADTPEQVLNFLHDL I I I I I I I : I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml28-l TRASELSDDGKFDNTANIDRTLANALQTAKLLGFKNYAELSLATKMADTPEQVLNFLHDL 250 260 270 280 290 300 310 320 330 340 350 360 gl28-l.pep ARRAKPYAEKDLAEVKAFAREHLGLADPQPWDLSYAGEKLREAKYAFSETEVKKYFPVGK I I I I I I I I I I I I I I I I I I I I I 1:111 I I I I I : I I : I I I I I I I I I I I I I I I I I I I I I I I ml28-l ARRAKPYAEKDLAEVKAFARESLNLADLQPWDLGYASEKLREAKYAFSETEVKKYFPVGK 310 320 330 340 350 360 370 380 390 400 410 420 gl28-l.pep VLAGLFAQIKKLYGIGFAEKTVPVWHKDVRYFELQQNGKTIGGVYMDLYAREGKRGGAWM II I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I ml28-l VLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYFELQQNGETIGGVYMDLYAREGKRGGAWM 370 380 390 400 410 420 430 440 450 460 470 480 gl28-l.pep NDYKGRRRFADGTLQLPTAYLVCNFAPPVGGKEARLSHDEILTLFHETGHGLHHLLTQVD I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I II I I I I I I I I ml28-l NDYKGRRRFSDGTLQLPTAYLVCNFAPPVGGREARLSHDEILILFHETGHGLHHLLTQVD 430 440 450 460 470 480 490 gl28-l.pep ELGVSGINGVK I II I I I I I I I : ml28-l ELGVSGINGVEWDAVELPSQFMENFVWEYNVLAQMSAHEETGVPLPKELFDKMLAAKNFQ 490 500 510 520 530 540 The following DNA sequence was identified in N. meningitidis <SEQ ID 3095>: al28-l.seq 1 ATGACTGACA ACGCACTGCT CCATTTGGGC GAAGAACCCC GTTTTGATCA 51 AATCAAAACC GAAGACATCA AACCCGCCCT GCAAACCGCC ATTGCCGAAG 101 CGCGCGAACA AATCGCCGCC ATCAAAGCCC AAACGCACAC CGGCTGGGCA 151 AACACTGTCG AACCCCTGAC CGGCATCACC GAACGCGTCG GCAGGATTTG 201 GGGCGTGGTG TCGCACCTCA ACTCCGTCAC CGACACGCCC GAACTGCGCG 251 CCGCCTACAA TGAATTAATG CCCGAAATTA CCGTCTTCTT CACCGAAATC 301 GGACAAGACA TCGAGCTGTA CAACCGCTTC AAAACCATCA AAAACTCCCC 351 CGAGTTCGAC ACCCTCTCCC ACGCGCAAAA AACCAAACTC AACCACGATC 401 TGCGCGATTT CGTCCTCAGC GGCGCGGAAC TGCCGCCCGA ACAGCAGGCA 451 GAATTGGCAA AACTGCAAAC CGAAGGCGCG CAACTTTCCG CCAAATTCTC 501 CCAAAACGTC CTAGACGCGA CCGACGCGTT CGGCATTTAC TTTGACGATG 551 CCGCACCGCT TGCCGGCATT CCCGAAGACG CGCTCGCCAT GTTTGCCGCT 601 GCCGCGCAAA GCGAAGGCAA AACAGGCTAC AAAATCGGTT TGCAGATTCC 651 GCACTACCTC GCCGTCATCC AATACGCCGA CAACCGCAAA CTGCGCGAAC 701 AAATCTACCG CGCCTACGTT ACCCGCGCCA GCGAGCTTTC AGACGACGGC 751 AAATTCGACA ACACCGCCAA CATCGACCGC ACGCTCGAAA ACGCCCTGCA 801 AACCGCCAAA CTGCTCGGCT TCAAAAACTA CGCCGAATTG TCGCTGGCAA 851 CCAAAATGGC GGACACCCCC GAACAAGTTT TAAACTTCCT GCACGACCTC 901 GCCCGCCGCG CCAAACCCTA CGCCGAAAAA GACCTCGCCG AAGTCAAAGC 951 CTTCGCCCGC GAAAGCCTCG GCCTCGCCGA TTTGCAACCG TGGGACTTGG 1001 GCTACGCCGG CGAAAAACTG CGCGAAGCCA AATACGCATT CAGCGAAACC 1051 GAAGTCAAAA AATACTTCCC CGTCGGCAAA GTATTAAACG GACTGTTCGC 1101 CCAAATCAAA AAACTCTACG GCATCGGATT TACCGAAAAA ACCGTCCCCG 1151 TCTGGCACAA AGACGTGCGC TATTTTGAAT TGCAACAAAA CGGCGAAACC 1201 ATAGGCGGCG TTTATATGGA TTTGTACGCA CGCGAAGGCA AACGCGGCGG 1251 CGCGTGGATG AACGACTACA AAGGCCGCCG CCGTTTTTCA GACGGCACGC 1301 TGCAACTGCC CACCGCCTAC CTCGTCTGCA ACTTCACCCC GCCCGTCGGC 1351 GGCAAAGAAG CCCGCTTGAG CCATGACGAA ATCCTCACCC TCTTCCACGA 1401 AACCGGACAC GGCCTGCACC ACCTGCTTAC CCAAGTCGAC GAACTGGGCG 1451 TATCCGGCAT CAACGGCGTA GAATGGGACG CAGTCGAACT GCCCAGTCAG 1501 TTTATGGAAA ATTTCGTTTG GGAATACAAT GTCTTGGCGC AAATGTCCGC 1551 CCACGAAGAA ACCGGCGTTC CCCTGCCGAA AGAACTCTTC GACAAAATGC 1601 TCGCCGCCAA AAACTTCCAA CGCGGAATGT TCCTCGTCCG CCAAATGGAG 1651 TTCGCCCTCT TTGATATGAT GATTTACAGC GAAGACGACG AAGGCCGTCT 1701 GAAAAACTGG CAACAGGTTT TAGACAGCGT GCGCAAAGAA GTCGCCGTCG 1751 TCCGACCGCC CGAATACAAC CGCTTCGCCA ACAGCTTCGG CCACATCTTC 1801 GCAGGCGGCT ATTCCGCAGG CTATTACAGC TACGCGTGGG CGGAAGTATT 1851 GAGCGCGGAC GCATACGCCG CCTTTGAAGA AAGCGACGAT GTCGCCGCCA 1901 CAGGCAAACG CTTTTGGCAG GAAATCCTCG CCGTCGGCGG ATCGCGCAGC 1951 GCGGCAGAAT CCTTCAAAGC CTTCCGCGGA CGCGAACCGA GCATAGACGC 2001 ACTCTTGCGC CACAGCGGCT TCGACAACGC GGCTTGA This corresponds to the amino acid sequence <SEQ LD 3096; ORF 128-1. a>: al28-l.pep 1 MTDNALLHLG EEPRFDQIKT EDIKPALQTA IAEAREQIAA IKAQTHTGWA 51 NTVEPLTGIT ERVGRIWGW SHLNSVTDTP ELRAAYNELM PEITVFFTEI 101 GQDIELYNRF KTIKNSPEFD TLSHAQKTKL NHDLRDFVLS GAELPPEQQA 151 ELAKLQTEGA QLSAKFSQNV LDATDAFGIY FDDAAPLAGI PEDALAMFAA 201 AAQSEGKTGY KIGLQIPHYL AVIQYADNRK LREQIYRAYV TRASELSDDG 251 KFDNTANIDR TLENALQTAK LLGFKNYAEL SLATKMADTP EQVLNFLHDL 301 ARRAKPYAEK DLAEVKAFAR ESLGLADLQP WDLGYAGEKL REAKYAFSET 351 EVKKYFPVGK VLNGLFAQIK KLYGIGFTEK TVPVWHKDVR YFELQQNGET 401 IGGVYMDLYA REGKRGGAWM NDYKGRRRFS DGTLQLPTAY LVCNFTPPVG 451 GKEARLSHDE ILTLFHETGH GLHHLLTQVD ELGVSGINGV EWDAVELPSQ 501 FMENFVWEYN VLAQMSAHEE TGVPLPKELF DKMLAAKNFQ RGMFLVRQME 551 FALFDMMIYS EDDEGRLKNW QQVLDSVRKE VAWRPPEYN RFANSFGHIF 601 AGGYSAGYYS YAWAEVLSAD AYAAFEESDD VAATGKRFWQ EILAVGGSRS 651 AAESFKAFRG REPSIDALLR HSGFDNAA* ml28-l/al28-l ORFs 128-1 and 128-1. a showed a 97.8% identity in 677 aa overlap 10 20 30 40 50 60 al28-l.pep MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQTHTGWANTVEPLTGIT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml28-l MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQTHTGWANTVEPLTGIT 10 20 30 40 50 60 70 80 90 100 110 120 al28-l.pep ERVGRIWGWSHLNSVTDTPELRAAYNELMPEITVFFTEIGQDIELYNRFKTIKNSPEFD I I I I I I I I I I I I I I I I: I I I I I I I: I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml28-l ERVGRIWGWSHLNSVADTPELRAVYNELMPEITVFFTEIGQDIELYNRFKTIKNSPEFD 70 80 90 100 110 120 130 140 150 160 170 180 al28-l . pep TLSHAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGAQLSAKFSQNVLDATDAFGIY III I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II ml28-l TLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGAQLSAKFSQNVLDATDAFGIY 130 140 150 160 170 180 190 200 210 220 230 240 al28-l.pep FDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPHYLAVIQYADNRKLREQIYRAYV I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I ml28-l FDDAAPLAGIPEDALAMFAAAAQSESKTGYKIGLQIPHYLAVIQYADNRELREQIYRAYV 190 200 210 220 230 240 250 260 270 280 290 300 al28-l . pep TRASELSDDGKFDNTANIDRTLENALQTAKLLGFKNYAELSLATKMADTPEQVLNFLHDL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I ml28-l TRASELSDDGKFDNTANIDRTLANALQTAKLLGFKNYAELSLATKMADTPEQVLNFLHDL 250 260 270 280 290 300 310 320 330 340 350 360 al28-l.pep ARRAKPYAEKDLAEVKAFARESLGLADLQPWDLGYAGEKLREAKYAFSETEVKKYFPVGK I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I : I I I I I I I I I I I I I I I I II I I I I I ml28-l ARRAKPYAEKDLAEVKAFARESLNLADLQPWDLGYASEKLREAKYAFSETEVKKYFPVGK 310 320 330 340 350 360 370 380 390 400 410 420 al28-l . pep VLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYFELQQNGETIGGVYMDLYAREGKRGGAWM I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml28-l VLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYFELQQNGETIGGVYMDLYAREGKRGGAWM 370 380 390 400 410 420 430 440 450 460 470 480 al28-l . pep NDYKGRRRFSDGTLQLPTAYLVCNFTPPVGGKEARLSHDEILTLFHETGHGLHHLLTQVD I I I I I I I I I I I I I I I I I I I I I I I I I: I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I ml28-l NDYKGRRRFSDGTLQLPTAYLVCNFAPPVGGREARLSHDEILILFHETGHGLHHLLTQVD 430 440 450 460 470 480 490 500 510 520 530 540 al28-l . pep ELGVSGINGVEWDAVELPSQFMENFVWEYNVLAQMSAHEETGVPLPKELFDKMLAAKNFQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml28-l ELGVSGINGVEWDAVELPSQFMENFVWEYNVLAQMSAHEETGVPLPKELFDKMLAAKNFQ 490 500 510 520 530 540 550 560 570 580 590 600 al28-l.pep RGMFLVRQMEFALFDMMIYSEDDEGRLKNWQQVLDSVRKEVAWRPPEYNRFANSFGHIF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I :: I II I I I I I llllll ml28-l RGMFLVRQMEFALFDMMIYSEDDEGRLKNWQQVLDSVRKKVAVIQPPEYNRFALSFGHIF 550 560 570 580 590 600 610 620 630 640 650 660 al28-l.pep AGGYSAGYYSYAWAEVLSADAYAAFEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml28-l AGGYSAGYYSYAWAEVLSADAYAAFEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRG 610 620 630 640 650 660 670 679 al28-l.pep REPSIDALLRHSGFDNAAX I I I I I I I I I I I I I I I I I : ml28-l REPSIDALLRHSGFDNAVX 670 206 The following partial DNA sequence was identified in N meningitidis <SEQ ID 3097>: m206 . seq 1 ATGTTTCCCC CCGACAAAAC CCTTTTCCTC TGTCTCAGCG CACTGCTCCT 51 CGCCTCATGC GGCACGACCT CCGGCAAACA CCGCCAACCG AAACCCAAAC 101 AGACAGTCCG GCAAATCCAA GCCGTCCGCA TCAGCCACAT CGACCGCACA 151 CAAGGCTCGC AGGAACTCAT GCTCCACAGC CTCGGACTCA TCGGCACGCC 201 CTACAAATGG GGCGGCAGCA GCACCGCAAC CGGCTTCGAT TGCAGCGGCA 251 TGATTCAATT CGTTTACAAr AACGCCCTCA ACGTCAAGCT GCCGCGCACC 301 GCCCGCGACA TGGCGGCGGC AAGCCGsAAA ATCCCCGAcA GCCGCyTCAA 351 GGCCGGCGAC CTCGTATTCT TCAACACCGG CGGCGCACAC CGCTACTCAC 401 ACGTCGGACT CTACATCGGC AACGGCGAAT TCATCCATGC CCCCAGCAGC 451 GGCAAAACCA TCAAAACCGA AAAACTCTCC ACACCGTTTT ACGCCAAAAA 501 CTACCTCGGC GCACATACTT TTTTTACAGA ATGA This corresponds to the amino acid sequence <SEQ ID 3098; ORF 206>: m206.pep.. 1 MFPPDKTLFL CLSALLLASC GTTSGKHRQP KPKQTVRQIQ AVRISHIDRT 51 QGSQELMLHS LGLIGTPYKW GGSSTATGFD CSGMIQFVYK NALNVKLPRT 101 ARDMAAASRK IPDSRXKAGD LVFFNTGGAH RYSHVGLYIG NGEFIHAPSS 151 GKTIKTEKLS TPFYAKNYLG AHTFFTE* The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 3099>: g206. seq 1 atgttttccc ccgacaaaac ccttttcctc tgtctcggcg cactgctcct 51 cgcctcatgc ggcacgacct ccggcaaaca ccgccaaccg aaacccaaac 101 agacagtccg gcaaatccaa gccgtccgca tcagccacat cggccgcaca 151 caaggctcgc aggaactcat gctccacagc ctcggactca tcggcacgcc 201 ctacaaatgg ggcggcagca gcaccgcaac cgg ttcgac tgcagcggca 251 tgattcaatt ggtttacaaa aacgccctca acgtcaagct gccgcgcacc 301 gcccgcgaca tggcggcggc aagccgcaaa atccccgaca gccgcctcaa 351 ggccggcgac atcgtattct tcaacaccgg cggcgcacac cgctactcac 401 acgtcggact ctacatcggc aacggcgaat tcatccatgc ccccggcagc 451 ggcaaaacca tcaaaaccga aaaactctcc acaccgtttt acgccaaaaa 501 ctaccttgga gcgcatacgt tttttacaga atga This corresponds to the amino acid sequence <SEQ ID 3100; ORF 206.ng>: g206.pep 1 MFSPDKTLFL CLGALLLASC GTTSGKHRQP KPKQTVRQIQ AVRISHIGRT 51 QGSQELMLHS LGLIGTPYKW GGSSTATGFD CSGMIQLVYK ΝALΝVKLPRT 101 ARDMAAASRK IPDSRLKAGD IVFFΝTGGAH RYSHVGLYIG ΝGEFIHAPGS 151 GKTIKTEKLS TPFYAKNYLG AHTFFTE* ORF 206 shows 96.0% identity over a 177 aa overlap with a predicted ORF (ORF 206.ng) from N gonorrhoeae: m206/g206 10 20 30 40 50 60 m206. ■Pep MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRISHIDRTQGSQELMLHS II MMMMhllMMMIMMIMMMMMMMMMM IIIMMMIM g206 MFSPDKTLFLCLGALLLASCGTTSGKHRQPKPKQTVRQIQAVRISHIGRTQGSQELMLHS 10 20 30 40 50 60 70 80 90 100 110 120 m206. •pep LGLIGTPYKWGGSSTATGFDCSGMIQFVYKΝALΝVKLPRTARDMAAASRKIPDSRXKAGD IMIMMMIMIMMMIMMhllMMMMMMIMIMIIMMM MM g206 LGLIGTPYKWGGSSTATGFDCSGMIQLVYKΝALΝVKLPRTARDMAAASRKIPDSRLKAGD 70 80 90 100 110 120 130 140 150 160 170 m206. •pep LVFFΝTGGAHRYSHVGLYIGΝGEFIHAPSSGKTIKTEKLSTPFYAKΝYLGAHTFFTEX MMMMMMMIMMMIMMIhlMIMMMMMMMIIMMMM g206 IVFFΝTGGAHRYSHVGLYIGΝGEFIHAPGSGKTIKTEKLSTPFYAKΝYLGAHTFFTE 130 140 150 160 170 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 3101>: a206. seq 1 ATGTTTCCCC CCGACAAAAC CCTTTTCCTC TGTCTCAGCG CACTGCTCCT 51 CGCCTCATGC GGCACGACCT CCGGCAAACA CCGCCAACCG AAACCCAAAC 101 AGACAGTCCG GCAAATCCAA GCCGTCCGCA TCAGCCACAT CGACCGCACA 151 CAAGGCTCGC AGGAACTCAT GCTCCACAGC CTCGGACTCA TCGGCACGCC 201 CTACAAATGG GGCGGCAGCA GCACCGCAAC CGGCTTCGAT TGCAGCGGCA 251 TGATTCAATT CGTTTACAAA AACGCCCTCA ACGTCAAGCT GCCGCGCACC 301 GCCCGCGACA TGGCGGCGGC AAGCCGCAAA ATCCCCGACA GCCGCCTTAA 351 GGCCGGCGAC CTCGTATTCT TCAACACCGG CGGCGCACAC CGCTACTCAC 401 ACGTCGGACT CTATATCGGC AACGGCGAAT TCATCCATGC CCCCAGCAGC 451 GGCAAAACCA TCAAAACCGA AAAACTCTCC ACACCGTTTT ACGCCAAAAA 501 CTACCTCGGC GCACATACTT TCTTTACAGA ATGA This corresponds to the amino acid sequence <SEQ ID 3102; ORF 206. a>: a206.pep 1 MFPPDKTLFL CLSALLLASC GTTSGKHRQP KPKQTVRQIQ AVRISHIDRT 51 QGSQELMLHS LGLIGTPYKW GGSSTATGFD CSGMIQFVYK ΝALΝVKLPRT 101 ARDMAAASRK IPDSRLKAGD LVFFΝTGGAH RYSHVGLYIG ΝGEFIHAPSS 151 GKTIKTEKLS TPFYAKNYLG AHTFFTE* m206/a206 ORFs 206 and 206.a showed a 99.4% identity in 177 aa overlap 10 20 30 40 50 60 m206.pep MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRISHIDRTQGSQELMLHS I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a206 MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRISHIDRTQGSQELMLHS 10 20 30 40 50 60 70 80 90 100 110 120 m206. pep LGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTARDMAAASRKIPDSRXKAGD I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I a206 LGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTARDMAAASRKIPDSRLKAGD 70 80 90 100 110 120 130 140 150 160 170 m206. pep LVFFNTGGAHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLSTPFYAKNYLGAHTFFTEX I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a206 LVFFNTGGAHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLSTPFYAKNYLGAHTFFTEX 130 140 150 160 170 287 The following partial DNA sequence was identified in N meningitidis <SEQ ID 3103>: m287. seq 1 ATGTTTAAAC GCAGCGTAAT CGCAATGGCT TGTATTTTTG CCCTTTCAGC 51 CTGCGGGGGC GGCGGTGGCG GATCGCCCGA TGTCAAGTCG GCGGACACGC 101 TGTCAAAACC TGCCGCCCCT GTTGTTTCTG AAAAAGAGAC AGAGGCAAAG 151 GAAGATGCGC CACAGGCAGG TTCTCAAGGA CAGGGCGCGC CATCCGCACA 201 AGGCAGTCAA GATATGGCGG CGGTTTCGGA AGAAAATACA GGCAATGGCG 251 GTGCGGTAAC AGCGGATAAT CCCAAAAATG AAGACGAGGT GGCACAAAAT 301 GATATGCCGC AAAATGCCGC CGGTACAGAT AGTTCGACAC CGAATCACAC 351 CCCGGATCCG AATATGCTTG CCGGAAATAT GGAAAATCAA GCAACGGATG 401 CCGGGGAATC GTCTCAGCCG GCAAACCAAC CGGATATGGC AAATGCGGCG 451 GACGGAATGC AGGGGGACGA TCCGTCGGCA GGCGGGCAAA ATGCCGGCAA 501 TACGGCTGCC CAAGGTGCAA ATCAAGCCGG AAACAATCAA GCCGCCGGTT 551 CTTCAGATCC CATCCCCGCG TCAAACCCTG CACCTGCGAA TGGCGGTAGC 601 AATTTTGGAA GGGTTGATTT GGCTAATGGC GTTTTGATTG ACGGGCCGTC 651 GCAAAATATA ACGTTGACCC ACTGTAAAGG CGATTCTTGT AGTGGCAATA 701 ATTTCTTGGA TGAAGAAGTA CAGCTAAAAT CAGAATTTGA AAAATTAAGT 751 GATGCAGACA AAATAAGTAA TTACAAGAAA GATGGGAAGA ATGATAAATT 801 TGTCGGTTTG GTTGCCGATA GTGTGCAGAT GAAGGGAATC AATCAATATA 851 TTATCTTTTA TAAACCTAAA CCCACTTCAT TTGCGCGATT TAGGCGTTCT 901 GCACGGTCGA GGCGGTCGCT TCCGGCCGAG ATGCCGCTGA TTCCCGTCAA 951 TCAGGCGGAT ACGCTGATTG TCGATGGGGA AGCGGTCAGC CTGACGGGGC 1001 ATTCCGGCAA TATCTTCGCG CCCGAAGGGA ATTACCGGTA TCTGACTTAC 1051 GGGGCGGAAA AATTGCCCGG CGGATCGTAT GCCCTTCGTG TTCAAGGCGA 1101 ACCGGCAAAA GGCGAAATGC TTGCGGGCGC GGCCGTGTAC AACGGCGAAG 1151 TACTGCATTT CCATACGGAA AACGGCCGTC CGTACCCGAC CAGGGGCAGG 1201 TTTGCCGCAA AAGTCGATTT CGGCAGCAAA TCTGTGGACG GCATTATCGA 1251 CAGCGGCGAT GATTTGCATA TGGGTACGCA AAAATTCAAA GCCGCCATCG 1301 ATGGAAACGG CTTTAAGGGG ACTTGGACGG AAAATGGCAG CGGGGATGTT 1351 TCCGGAAAGT TTTACGGCCC GGCCGGCGAG GAAGTGGCGG GAAAATACAG 1401 CTATCGCCCG ACAGATGCGG AAAAGGGCGG ATTCGGCGTG TTTGCCGGCA 1451 AAAAAGAGCA GGATTGA This corresponds to the amino acid sequence <SEQ ID 3104; ORF 287>: m287.pep 1 MFKRSVIAMA CIFALSACGG GGGGSPDVKS ADTLSKPAAP WSEKETEAK 51 EDAPQAGSQG QGAPSAQGSQ DMAAVSEENT GNGGAVTADN PKNEDEVAQN 101 DMPQNAAGTD SSTPNHTPDP NMLAGNMENQ ATDAGESSQP ANQPDMANAA 151 DGMQGDDPSA GGQNAGNTAA QGANQAGNNQ AAGSSDPIPA SNPAPANGGS 201 NFGRVDLANG VLIDGPSQNI TLTHCKGDSC SGNNFLDEEV QLKSEFEKLΞ 251 DADKISNYKK DGKNDKFVGL VADSVQMKGI NQYIIFYKPK PTSFARFRRS 301 ARSRRSLPAE MPLIPVNQAD TLIVDGEAVS LTGHSGNIFA PEGNYRYLTY 351 GAEKLPGGSY ALRVQGEPAK GEMLAGAAVY NGEVLHFHTE NGRPYPTRGR 401 FAAKVDFGSK SVDGIIDSGD DLHMGTQKFK AAIDGNGFKG TWTENGSGDV 451 SGKFYGPAGE EVAGKYSYRP TDAEKGGFGV FAGKKEQD* The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 3105>: g287.seq 1 atgtttaaac gcagtgtgat tgcaatggct tgtatttttc ccctttcagc 51 ctgtgggggc ggcggtggcg gatcgcccga tgtcaagtcg gcggacacgc 101 cgtcaaaacc ggccgccccc gttgttgctg aaaatgccgg ggaaggggtg 151 ctgccgaaag aaaagaaaga tgaggaggca gcgggcggtg cgccgcaagc 201 cgatacgcag gacgcaaccg ccggagaagg cagccaagat atggcggcag 251 tttcggcaga aaatacaggc aatggcggtg cggcaacaac ggacaacccc 301 aaaaatgaag acgcgggggc gcaaaatgat atgccgcaaa atgccgccga 351 atccgcaaat caaacaggga acaaccaacc cgccggttct tcagattccg 401 cccccgcgtc aaaccctgcc cctgcgaatg gcggtagcga ttttggaagg 451 acgaacgtgg gcaattctgt tgtgattgac ggaccgtcgc aaaatataac 501 gttgacccac tgtaaaggcg attcttgtaa tggtgataat ttattggatg 551 aagaagcacc gtcaaaatca gaatttgaaa aattaagtga tgaagaaaaa 601 attaagcgat ataaaaaaga cgagcaacgg gagaattttg tcggtttggt 651 tgctgacagg gtaaaaaagg atggaactaa caaatatatc atcttctata 701 cggacaaacc acctactcgt tctgcacggt cgaggaggtc gcttccggcc 751 gagattccgc tgattcccgt caatcaggcc gatacgctga ttgtggatgg 801 ggaagcggtc agcctgacgg ggcattccgg caatatcttc gcgcccgaag 851 ggaattaccg gtatctgact tacggggcgg aaaaattgcc cggcggatcg 901 tatgccctcc gtgtgcaagg cgaaccggca aaaggcgaaa tgcttgttgg 951 cacggccgtg tacaacggcg aagtgctgca tttccatatg gaaaacggcc 1001 gtccgtaccc gtccggaggc aggtttgccg caaaagtcga tttcggcagc 1051 aaatctgtgg acggcattat cgacagcggc gatgatttgc atatgggtac 1101 gcaaaaattc aaagccgcca tcgatggaaa cggctttaag gggacttgga 1151 cggaaaatgg cggcggggat gtttccggaa ggttttacgg cccggccggc 1201 gaggaagtgg cgggaaaata cagctatcgc ccgacagatg ctgaaaaggg 1251 cggattcggc gtgtttgccg gcaaaaaaga tcgggattga This corresponds to the amino acid sequence <SEQ ID 3106; ORF 287.ng>: g287 . pep 1 MFKRSVIAMA CIFPLSACGG GGGGSPDVKS ADTPSKPAAP WAEΝAGEGV 51 LPKEKKDEEA AGGAPQADTQ DATAGEGSQD MAAVSAENTG NGGAATTDNP 101 KNEDAGAQND MPQNAAESAN QTGNNQPAGS SDSAPASNPA PANGGSDFGR 151 TNVGNSWID GPSQNITLTH CKGDSCNGDN LLDEEAPSKS EFEKLSDEEK 201 IKRYKKDEQR ENFVGLVADR VKKDGTNKYI IFYTDKPPTR SARSRRSLPA 251 EIPLIPVNQA DTLIVDGEAV SLTGHSGNIF APEGNYRYLT YGAEKLPGGS 301 YALRVQGEPA KGEMLVGTAV YNGEVLHFHM ENGRPYPSGG RFAAKVDFGS 351 KSVDGIIDSG DDLHMGTQKF KAAIDGNGFK GTWTENGGGD VSGRFYGPAG 401 EEVAGKYSYR PTDAEKGGFG VFAGKKDRD* /g287 ORFs 287 and 287.ng showed a 70.1% identity in 499 aa overlap 10 20 30 40 49 m287.pep MFKRSVIAMACIFALSACGGGGGGSPDVKSADTLSKPAAPWSE KETEA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II 11111111:1 I : II g287 MFKRSVIAMACIFPLSACGGGGGGSPDVKSADTPSKPAAPWAENAGEGVLPKEKKDEEA 10 20 30 40 50 60 50 60 70 80 90 100 109 m287. pep KEDAPQAGSQGQGAPSAQGSQDMAAVSEENTGNGGAVTADNPKNEDEVAQNDMPQNAAGT I I I I : I I ::: I I I I I I I I I I I I I I I I I : I : I I I I I I I I I I I I I I I I I g287 AGGAPQADTQD—ATAGEGSQDMAAVSAENTGNGGAATTDNPKNEDAGAQNDMPQNAA— 70 80 90 100 110 110 120 130 140 150 160 169 m287. pep DSSTPNHTPDPNMLAGNMENQATDAGESSQPANQPDMANAADGMQGDDPSAGGQNAGNTA g287 170 180 190 200 210 220 229 m287. pep AQGANQAGNNQAAGSSDPIPASNPAPANGGSNFGRVDLANGVLIDGPSQNITLTHCKGDS :: I I I : I I I I I I I I I I I I I I I I I I I I I : I I I : :: : I : I : I I I I I I I I I I I I I I I I I g287 -ESANQTGNNQPAGΞSDSAPASNPAPANGGSDFGRTNVGNSWIDGPSQNITLTHCKGDS 120 130 140 150 160 170 230 240 250 260 270 280 289 m287. pep CSGNNFLDEEVQLKSEFEKLSDADKISNYKKDGKNDKFVGLVADSVQMKGINQYIIFYKP I : I : I : I I I I : I I I I I I I I I : I I : I I I I : : : I I I I I I I I : I I : I I I I I g287 CNGDNLLDEEAPSKSEFEKLSDEEKIKRYKKDEQRENFVGLVADRVKKDGTNKYIIFYTD 180 190 200 210 220 230 290 300 310 320 330 340 349 m287. ■ pep KPTSFARFRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGNIFAPEGNYRYLT II : II I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g287 KPPT RSARSRRSLPAEIPLIPVNQADTLIVDGEAVSLTGHSGNIFAPEGNYRYLT 240 250 260 270 280 290 350 360 370 380 390 400 409 m287 • pep YGAEKLPGGSYALRVQGEPAKGEMLAGAAVYNGEVLHFHTENGRPYPTRGRFAAKVDFGS I I I I I I I I I I I I I I I I I I I I I I I I I : I : I I I I I I I I I I I lllllll: I I I I I I I I I I I g287 YGAEKLPGGSYALRVQGEPAKGEMLVGTAVYNGEVLHFHMENGRPYPSGGRFAAKVDFGS 300 310 320 330 340 350 410 420 430 440 450 460 469 m287.pep KSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGTWTENGSGDVSGKFYGPAGEEVAGKYSYR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I: I I I I I: I I I I I I I I I I I I I I I I g287 KSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGTWTENGGGDVSGRFYGPAGEEVAGKYSYR 360 370 380 390 400 410 470 480 489 m287. pep PTDAEKGGFGVFAGKKEQDX I I I I I I I I I I I I I I I I :: I I g287 PTDAEKGGFGVFAGKKDRDX 420 430 The following partial DNA sequence was identified in N meningitidis <SEQ ID 3107>: a287.seq 1 ATGTTTAAAC GCAGTGTGAT TGCAATGGCT TGTATTGTTG CCCTTTCAGC 51 CTGTGGGGGC GGCGGTGGCG GATCGCCCGA TGTTAAGTCG GCGGACACGC 101 TGTCAAAACC TGCCGCCCCT GTTGTTACTG AAGATGTCGG GGAAGAGGTG 151 CTGCCGAAAG AAAAGAAAGA TGAGGAGGCG GTGAGTGGTG CGCCGCAAGC 201 CGATACGCAG GACGCAACCG CCGGAAAAGG CGGTCAAGAT ATGGCGGCAG 251 TTTCGGCAGA AAATACAGGC AATGGCGGTG CGGCAACAAC GGATAATCCC 301 GAAAATAAAG ACGAGGGACC GCAAAATGAT ATGCCGCAAA ATGCCGCCGA 351 TACAGATAGT TCGACACCGA ATCACACCCC TGCACCGAAT ATGCCAACCA 401 GAGATATGGG AAACCAAGCA CCGGATGCCG GGGAATCGGC ACAACCGGCA 451 AACCAACCGG ATATGGCAAA TGCGGCGGAC GGAATGCAGG GGGACGATCC 501 GTCGGCAGGG GAAAATGCCG GCAATACGGC AGATCAAGCT GCAAATCAAG 551 CTGAAAACAA TCAAGTCGGC GGCTCTCAAA ATCCTGCCTC TTCAACCAAT 601 CCTAACGCCA CGAATGGCGG CAGCGATTTT GGAAGGATAA ATGTAGCTAA 651 TGGCATCAAG CTTGACAGCG GTTCGGAAAA TGTAACGTTG ACACATTGTA 701 AAGACAAAGT ATGCGATAGA GATTTCTTAG ATGAAGAAGC ACCACCAAAA 751 TCAGAATTTG AAAAATTAAG TGATGAAGAA AAAATTAATA AATATAAAAA 801 AGACGAGCAA CGAGAGAATT TTGTCGGTTT GGTTGCTGAC AGGGTAGAAA 851 AGAATGGAAC TAACAAATAT GTCATCATTT ATAAAGACAA GTCCGCTTCA 901 TCTTCATCTG CGCGATTCAG GCGTTCTGCA CGGTCGAGGC GGTCGCTTCC 951 GGCCGAGATG CCGCTGATTC CCGTCAATCA GGCGGATACG CTGATTGTCG 1001 ATGGGGAAGC GGTCAGCCTG ACGGGGCATT CCGGCAATAT CTTCGCGCCC 1051 GAAGGGAATT ACCGGTATCT GACTTACGGG GCGGAAAAAT TGTCCGGCGG 1101 ATCGTATGCC CTCAGTGTGC AAGGCGAACC GGCAAAAGGC GAAATGCTTG 1151 CGGGCACGGC CGTGTACAAC GGCGAAGTGC TGCATTTCCA TATGGAAAAC 1201 GGCCGTCCGT CCCCGTCCGG AGGCAGGTTT GCCGCAAAAG TCGATTTCGG 1251 CAGCAAATCT GTGGACGGCA TTATCGACAG CGGCGATGAT TTGCATATGG 1301 GTACGCAAAA ATTCAAAGCC GTTATCGATG GAAACGGCTT TAAGGGGACT 1351 TGGACGGAAA ATGGCGGCGG GGATGTTTCC GGAAGGTTTT ACGGCCCGGC 1401 CGGCGAAGAA GTGGCGGGAA AATACAGCTA TCGCCCGACA GATGCGGAAA 1451 AGGGCGGATT CGGCGTGTTT GCCGGCAAAA AAGAGCAGGA TTGA This corresponds to the amino acid sequence <SEQ ID 3108; ORF 287. a>: a287.pep 1 MFKRSVIAMA CIVALSACGG GGGGSPDVKS ADTLSKPAAP VVTEDVGEEV 51 LPKEKKDEEA VSGAPQADTQ DATAGKGGQD MAAVSAEΝTG ΝGGAATTDΝP 101 EΝKDEGPQΝD MPQΝAADTDS STPΝHTPAPΝ MPTRDMGΝQA PDAGESAQPA 151 ΝQPDMAΝAAD GMQGDDPSAG EΝAGΝTADQA AΝQAEΝΝQVG GSQΝPASSTΝ 201 PΝATΝGGSDF GRIΝVAΝGIK LDSGSEΝVTL THCKDKVCDR DFLDEEAPPK 251 SEFEKLSDEE KIΝKYKKDEQ REΝFVGLVAD RVEKΝGTΝKY VIIYKDKSAS 301 SSSARFRRSA RSRRSLPAEM PLIPVΝQADT LIVDGEAVSL TGHSGΝIFAP 351 EGΝYRYLTYG AEKLSGGSYA LSVQGEPAKG EMLAGTAVYΝ GEVLHFHMEΝ 401 GRPSPSGGRF AAKVDFGSKS VDGIIDSGDD LHMGTQKFKA VIDGΝGFKGT 451 WTEΝGGGDVS GRFYGPAGEE VAGKYSYRPT DAEKGGFGVF AGKKEQD* m287/a287 ORFs 287 and 287. a showed a 77.2% identity in 501 aa overlap 10 20 30 40 49 m287.pep MFKRSVIAMACIFALSACGGGGGGSPDVKSADTLSKPAAPWSE KETEA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I : II a287 MFKRSVIAMACIVALSACGGGGGGSPDVKSADTLSKPAAPWTEDVGEEVLPKEKKDEEA 10 20 30 40 50 60 50 60 70 80 90 100 109 m287.pep KEDAPQAGSQGQGAPSAQGSQDMAAVSEEΝTGΝGGAVTADΝPKΝEDEVAQΝDMPQΝAAGT II I I : I I ::: I : I I I I I I I I I I I I I I I : I : II I : I : I | I I I I I I I I I I a287 VSGAPQADTQ—DATAGKGGQDMAAVSAEΝTGΝGGAATTDΝPEΝKDEGPQΝDMPQΝAADT 70 80 90 100 110 110 120 130 140 150 160 169 m287. pep DSSTPNHTPDPNMLAGNMENQATDAGESSQPANQPDMANAADGMQGDDPSAGGQNAGNTA I I I I I I I I I III : : I III I I I I I : I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I a287 DSSTPNHTPAPNMPTRDMGNQAPDAGESAQPANQPDMANAADGMQGDDPSAG-ENAGNTA 120 130 140 150 160 170 170 180 190 200 210 220 229 m287.pep AQGANQAGNNQAAGSSDPIPASNPAPANGGSNFGRVDLANGVLIDGPSQNITLTHCKGDS I : I I I I I I I : : I I : : I : : I I : I I I I : I I I : : : I I I : : I : I : I : I I I I I I a287 DQAANQAENNQVGGSQNPASSTNPNATNGGSDFGRINVANGIKLDSGSENVTLTHCKDKV 180 190 200 210 220 230 230 240 250 260 270 280 289 m287. pep CSGNNFLDEEVQLKSEFEKLSDADKISNYKKDGKNDKFVGLVADSVQMKGINQYIIFYKP I : : I I I I I : I I I I I I I I I : I I : : I I I I : : : I I I I I I I I : : I I : I : I : I I a287 CD-RDFLDEEAPPKSEFEKLSDEEKINKYKKDEQRENFVGLVADRVEKNGTNKYVIIYKD 240 250 260 270 280 290 290 300 310 320 330 340 m287.pep KP—TSFARFRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGNIFAPEGNYRY I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a287 KSASSSSARFRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGNIFAPEGNYRY 300 310 320 330 340 350 350 360 370 380 390 400 m28 . pep LTYGAEKLPGGSYALRVQGEPAKGEMLAGAAVYNGEVLHFHTENGRPYPTRGRFAAKVDF I I I I I I I I llllll I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I : I I I I I I I I I a287 LTYGAEKLSGGSYALSVQGEPAKGEMLAGTAVYNGEVLHFHMENGRPSPSGGRFAAKVDF 360 370 380 390 400 410 410 420 430 440 450 460 m287. pep GSKSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGTWTENGSGDVSGKFYGPAGEEVAGKYS I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I : I I I I I : I I I I I I I I I I I I I I a287 GSKSVDGIIDSGDDLHMGTQKFKAVIDGNGFKGTWTENGGGDVSGRFYGPAGEEVAGKYS 420 430 440 450 460 470 470 480 489 m287.pep YRPTDAEKGGFGVFAGKKEQDX I I I I I I I I I I I I I I I I I I I I I I a287 YRPTDAEKGGFGVFAGKKEQDX 480 490 406 The following partial DNA sequence was identified in N meningitidis <SEQ ID 3109>: m406. seq 1 ATGCAAGCAC GGCTGCTGAT ACCTATTCTT TTTTCAGTTT TTATTTTATC 51 CGCCTGCGGG ACACTGACAG GTATTCCATC GCATGGCGGA GGTAAACGCT 101 TTGCGGTCGA ACAAGAACTT GTGGCCGCTT CTGCCAGAGC TGCCGTTAAA 151 GACATGGATT TACAGGCATT ACACGGACGA AAAGTTGCAT TGTACATTGC 201 CACTATGGGC GACCAAGGTT CAGGCAGTTT GACAGGGGGT CGCTACTCCA 251 TTGATGCACT GATTCGTGGC GAATACATAA ACAGCCCTGC CGTCCGTACC 301 GATTACACCT ATCCACGTTA CGAAACCACC GCTGAAACAA CATCAGGCGG 351 TTTGACAGGT TTAACCACTT CTTTATCTAC ACTTAATGCC CCTGCACTCT 401 CTCGCACCCA ATCAGACGGT AGCGGAAGTA AAAGCAGTCT GGGCTTAAAT 451 ATTGGCGGGA TGGGGGATTA TCGAAATGAA ACCTTGACGA CTAACCCGCG 501 CGACACTGCC TTTCTTTCCC ACTTGGTACA GACCGTATTT TTCCTGCGCG 551 GCATAGACGT TGTTTCTCCT GCCAATGCCG ATACAGATGT GTTTATTAAC 601 ATCGACGTAT TCGGAACGAT ACGCAACAGA ACCGAAATGC ACCTATACAA 651 TGCCGAAACA CTGAAAGCCC AAACAAAACT GGAATATTTC GCAGTAGACA 701 GAACCAATAA AAAATTGCTC ATCAAACCAA AAACCAATGC GTTTGAAGCT 751 GCCTATAAAG AAAATTACGC ATTGTGGATG GGGCCGTATA AAGTAAGCAA 801 AGGAATTAAA CCGACGGAAG GATTAATGGT CGATTTCTCC GATATCCGAC 851 CATACGGCAA TCATACGGGT AACTCCGCCC CATCCGTAGA GGCTGATAAC 901 AGTCATGAGG GGTATGGATA CAGCGATGAA GTAGTGCGAC AACATAGACA 951 AGGACAACCT TGA This corresponds to the amino acid sequence <SEQ ID 3110; ORF 406>: 406. ep 1 MQARLLIPIL FSVFILSACG TLTGIPSHGG GKRFAVEQEL VAASARAAVK 51 DMDLQALHGR KVALYIATMG DQGSGSLTGG RYSIDALIRG EYINSPAVRT 101 DYTYPRYETT AETTSGGLTG LTTSLSTLNA PALSRTQSDG SGSKSSLGLN 151 IGGMGDYRNE TLTTNPRDTA FLSHLVQTVF FLRGIDWSP ANADTDVFIN 201 IDVFGTIRNR TEMHLYNAET LKAQTKLEYF AVDRTNKKLL IKPKTNAFEA 251 AYKENYALWM GPYKVSKGIK PTEGLMVDFS DIRPYGNHTG NSAPSVEADN 301 SHEGYGYSDE WRQHRQGQP * The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 3111>: g406.seq 1 ATGCGGGCAC GGCTGCTGAT ACCTATTCTT TTTTCAGTTT TTATTTTATC 51 CGCCTGCGGG ACACTGACAG GTATTCCATC GCATGGCGGA GGCAAACGCT 101 TCGCGGTCGA ACAAGAACTT GTGGCCGCTT CTGCCAGAGC TGCCGTTAAA 151 GACATGGATT TACAGGCATT ACACGGACGA AAAGTTGCAT TGTACATTGC 201 AACTATGGGC GACCAAGGTT CAGGCAGTTT GACAGGGGGT CGCTACTCCA 251 TTGATGCACT GATTCGCGGC GAATACATAA ACAGCCCTGC CGTCCGCACC 301 GATTACACCT ATCCGCGTTA CGAAACCACC GCTGAAACAA CATCAGGCGG 351 TTTGACGGGT TTAACCACTT CTTTATCTAC ACTTAATGCC CCTGCACTCT 401 CGCGCACCCA ATCAGACGGT AGCGGAAGTA GGAGCAGTCT GGGCTTAAAT 451 ATTGGCGGGA TGGGGGATTA TCGAAATGAA ACCTTGACGA CCAACCCGCG 501 CGACACTGCC TTTCTTTCCC ACTTGGTGCA GACCGTATTT TTCCTGCGCG 551 GCATAGACGT TGTTTCTCCT GCCAATGCCG ATACAGATGT GTTTATTAAC 601 ATCGACGTAT TCGGAACGAT ACGCAACAGA ACCGAAATGC ACCTATACAA 651 TGCCGAAACA CTGAAAGCCC AAACAAAACT GGAATATTTC GCAGTAGACA 701 GAACCAATAA AAAATTGCTC ATCAAACCCA AAACCAATGC GTTTGAAGCT 751 GCCTATAAAG AAAATTACGC ATTGTGGATG GGGCCGTATA AAGTAAGCAA 801 AGGAATCAAA CCGACGGAAG GATTGATGGT CGATTTCTCC GATATCCAAC 851 CATACGGCAA TCATACGGGT AACTCCGCCC CATCCGTAGA GGCTGATAAC 901 AGTCATGAGG GGTATGGATA CAGCGATGAA GCAGTGCGAC AACATAGACA 951 AGGGCAACCT TGA This corresponds to the amino acid sequence <SEQ ID 3112; ORF 406>: g406.pep 1 MRARLLIPIL FSVFILSACG TLTGIPSHGG GKRFAVEQEL VAASARAAVK 51 DMDLQALHGR KVALYIATMG DQGSGSLTGG RYSIDALIRG EYINSPAVRT 101 DYTYPRYETT AETTSGGLTG LTTSLSTLNA PALSRTQSDG SGSRSSLGLN 151 IGGMGDYRNE TLTTNPRDTA FLSHLVQTVF FLRGIDWSP ANADTDVFIN 201 IDVFGTIRNR TEMHLYNAET LKAQTKLEYF AVDRTNKKLL IKPKTNAFEA 251 AYKENYALWM GPYKVSKGIK PTEGLMVDFS DIQPYGNHTG NSAPSVEADN 301 SHEGYGYSDE AVRQHRQGQP * ORF 406 shows 98.8% identity over a 320 aa overlap with a predicted ORF (ORF406.a) from N gonorrhoeae: g406/m406 10 20 30 40 50 60 g406.pep MRARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAASARAAVKDMDLQALHGR 1 = 1 I I I M i l l I I I I I I I I l l l l l l I I I I I M l I I I M M I I M i l l M M I I I I I I I I I m406 MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAASARAAVKDMDLQALHGR 10 20 30 40 50 60 70 80 90 100 110 120 g406.pep KVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDYTYPRYETTAETTSGGLTG MMMMMMMMMMMMMMMMMMMIMMMMMMIMMMM m406 KVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDYTYPRYETTAETTSGGLTG 70 80 90 100 110 120 130 140 150 160 170 180 g406.pep LTTSLSTLNAPALSRTQSDGSGSRSSLGLNIGGMGDYRNETLTTNPRDTAFLSHLVQTVF m406 LTTSLSTLNAPALSRTQSDGSGSKSSLGLNIGGMGDYRNETLTTNPRDTAFLSHLVQTVF 130 140 150 160 170 180 190 200 210 220 230 240 g406. pep FLRGIDWSPANADTDVFINIDVFGTIRNRTEMHLYNAETLKAQTKLEYFAVDRTNKKLL MMMMMMMMMMMMMMMIMIMIMMMMMMMIMMMM m406 FLRGIDWSPANADTDVFINIDVFGTIRNRTEMHLYNAETLKAQTKLEYFAVDRTNKKLL 190 200 210 220 230 240 250 260 270 280 290 300 g406. pep IKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLMVDFSDIQPYGNHTGNSAPSVEADN MMMMIMMMMMMMMMMMMMIMMIMMMMMMIIMIM m406 IKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLMVDFSDIRPYGNHTGNSAPSVEADN 250 260 270 280 290 300 310 320 g406.pep SHEGYGYSDEAVRQHRQGQPX I II I II 1111 H II 1111111 m406 SHEGYGYSDEWRQHRQGQPX 310 320 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 3113>: a406. seq 1 ATGCAAGCAC GGCTGCTGAT ACCTATTCTT TTTTCAGTTT TTATTTTATC 51 CGCCTGCGGG ACACTGACAG GTATTCCATC GCATGGCGGA GGTAAACGCT 101 TCGCGGTCGA ACAAGAACTT GTGGCCGCTT CTGCCAGAGC TGCCGTTAAA 151 GACATGGATT TACAGGCATT ACACGGACGA AAAGTTGCAT TGTACATTGC 201 AACTATGGGC GACCAAGGTT CAGGCAGTTT GACAGGGGGT CGCTACTCCA 251 TTGATGCACT GATTCGTGGC GAATACATAA ACAGCCCTGC CGTCCGTACC 301 GATTACACCT ATCCACGTTA CGAAACCACC GCTGAAACAA CATCAGGCGG 351 TTTGACAGGT TTAACCACTT CTTTATCTAC ACTTAATGCC CCTGCACTCT 401 CGCGCACCCA ATCAGACGGT AGCGGAAGTA AAAGCAGTCT GGGCTTAAAT 451 ATTGGCGGGA TGGGGGATTA TCGAAATGAA ACCTTGACGA CTAACCCGCG 501 CGACACTGCC TTTCTTTCCC ACTTGGTACA GACCGTATTT TTCCTGCGCG 551 GCATAGACGT TGTTTCTCCT GCCAATGCCG ATACGGATGT GTTTATTAAC 601 ATCGACGTAT TCGGAACGAT ACGCAACAGA ACCGAAATGC ACCTATACAA 651 TGCCGAAACA CTGAAAGCCC AAACAAAACT GGAATATTTC GCAGTAGACA 701 GAACCAATAA AAAATTGCTC ATCAAACCAA AAACCAATGC GTTTGAAGCT 751 GCCTATAAAG AAAATTACGC ATTGTGGATG GGACCGTATA AAGTAAGCAA 801 AGGAATTAAA CCGACAGAAG GATTAATGGT CGATTTCTCC GATATCCAAC 851 CATACGGCAA TCATATGGGT AACTCTGCCC CATCCGTAGA GGCTGATAAC 901 AGTCATGAGG GGTATGGATA CAGCGATGAA GCAGTGCGAC GACATAGACA 951 AGGGCAACCT TGA This corresponds to the amino acid sequence <SEQ ID 3114; ORF 406.a>: a406.pep 1 MQARLLIPIL FSVFILSACG TLTGIPSHGG GKRFAVEQEL VAASARAAVK 51 DMDLQALHGR KVALYIATMG DQGSGSLTGG RYSIDALIRG EYINSPAVRT 101 DYTYPRYETT AETTSGGLTG LTTSLSTLNA PALSRTQSDG SGSKSSLGLN 151 IGGMGDYRNE TLTTNPRDTA FLSHLVQTVF FLRGIDWSP ANADTDVFIN 201 IDVFGTIRNR TEMHLYNAET LKAQTKLEYF AVDRTNKKLL IKPKTNAFEA 251 AYKENYALWM GPYKVSKGIK PTEGLMVDFS DIQPYGNHMG NSAPSVEADN 301 SHEGYGYSDE AVRRHRQGQP * m406/a406 ORFs 406 and 406. a showed a 98.8% identity in 320 aa overlap 10 20 30 40 50 60 m406.pep MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAASARAAVKDMDLQALHGR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a406 MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAASARAAVKDMDLQALHGR 10 20 30 40 50 60 70 80 90 100 110 120 m406.pep KVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDYTYPRYETTAETTSGGLTG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a406 KVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDYTYPRYETTAETTSGGLTG 70 80 90 100 110 120 130 140 150 160 170 180 m406.pep LTTSLSTLNAPALSRTQSDGSGSKSSLGLNIGGMGDYRNETLTTNPRDTAFLSHLVQTVF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a406 LTTSLSTLNAPALSRTQSDGSGSKSSLGLNIGGMGDYRNETLTTNPRDTAFLSHLVQTVF 130 140 150 160 170 180 190 200 210 220 230 240 m406. pep FLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNAETLKAQTKLEYFAVDRTNKKLL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I a406 FLRGIDWSPANADTDVFINIDVFGTIRNRTEMHLYNAETLKAQTKLEYFAVDRTNKKLL 190 200 210 220 230 240 250 260 270 280 290 300 m406.pep IKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLMVDFSDIRPYGNHTGNSAPSVEADN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I a406 IKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLMVDFSDIQPYGNHMGNSAPSVEADN 250 260 270 280 290 300 310 320 m406.pep SHEGYGYSDEWRQHRQGQPX I I I I I I I I I I : I I : I I I I I I I a406 SHEGYGYSDEAVRRHRQGQPX 310 320 EXAMPLE 2 Expression of ORF 919 The primer described in Table 1 for ORF 919 was used to locate and clone ORF 919. The predicted gene 919 was cloned in pET vector and expressed in E. coli. The product of protein expression and purification was analyzed by SDS-PAGE. In panel A) is shown the analysis of 919-His fusion protein purification. Mice were immunized with the purified 919- His and sera were used for Western blot (panel B), FACS analysis (panel C), bactericidal assay (panel D), and ELISA assay (panel E). Symbols: Ml, molecular weight marker; PP, purified protein, TP, N meningitidis total protein extract; OMV, N. meningitidis outer membrane vesicle preparation. Arrows indicate the position of the main recombinant protein product (A) and the N meningitidis immunoreactive band (B). These experiments confirm that 919 is a surface-exposed protein and that it is a useful immunogen. The hydrophilicity plots, antigenic index, and amphipatic regions of ORF 919 are provided in Figure 10. The AMPHI program is used to predict putative T-cell epitopes (Gao et al 1989, J. Immunol 143:3007; Roberts et al. 1996, AIDS Res Human Retroviruses 12:593; Quakyi et al. 1992, Scand J Immunol Suppl 11 :9). The nucleic acid sequence of ORF 919 and the amino acid sequence encoded thereby is provided in Example 1. EXAMPLE 3 Expression of ORF 279 The primer described in Table 1 for ORF 279 was used to locate and clone ORF 279. The predicted gene 279 was cloned in pGex vector and expressed in E. coli. The product of protein expression and purification was analyzed by SDS-PAGE. In panel A) is shown the analysis of 279-GST purification. Mice were immunized with the purified 279-GST and sera were used for Western blot analysis (panel B), FACS analysis (panel C), bactericidal assay (panel D), and ELISA assay (panel E). Symbols: Ml, molecular weight marker; TP, N meningitidis total protein extract; OMV, N. meningitidis outer membrane vescicle preparation. Arrows indicate the position of the main recombinant protein product (A) and the N meningitidis immunoreactive band (B). These experiments confirm that 279 is a surface- exposed protein and that it is a useful immunogen. The hydrophilicity plots, antigenic index, and amphipatic regions of ORF 279 are provided in Figure 11. The AMPHI program is used to predict putative T-cell epitopes (Gao et al 1989, J. Immunol 143:3007; Roberts et al. 1996, AIDS Res Human Retroviruses 12:593; Quakyi et al. 1992, Scand J Immunol Suppl 11:9). The nucleic acid sequence of ORF 279 and the amino acid sequence encoded thereby is provided in Example 1. EXAMPLE 4 Expression of ORF 576 and 576-1 The primer described in Table 1 for ORF 576 was used to locate and clone ORF 576. The predicted gene 576 was cloned in pGex vector and expressed in E. coli. The product of protein purification was analyzed by SDS-PAGE. In panel A) is shown the analysis of 576- GST fusion protein purification. Mice were immunized with the purified 576-GST and sera were used for Western blot (panel B), FACS analysis (panel C), bactericidal assay (panel D), and ELISA assay (panel E). Symbols: Ml, molecular weight marker; TP, N meningitidis total protein extract; OMV, N meningitidis outer membrane vescicle preparation. Arrows indicate the position of the main recombinant protein product (A) and the N meningitidis immunoreactive band (B).. These experiments confirm that ORF 576 is a surface-exposed protein and that it is a useful immunogen. The hydrophilicity plots, antigenic index, and amphipatic regions of ORF 576 are provided in Figure 12. The AMPHI program is used to predict putative T-cell epitopes (Gao et al 1989, J. Immunol 143:3007; Roberts et al. 1996, AIDS Res Human Retroviruses 12:593; Quakyi et al. 1992, Scand J Immunol Suppl 11:9). The nucleic acid sequence of ORF 576 and the amino acid sequence encoded thereby is provided in Example 1. EXAMPLE 5 Expression of ORF 519 and 519-1 The primer described in Table 1 for ORF 519 was used to locate and clone ORF 519. The predicted gene 519 was cloned in pET vector and expressed in E. coli. The product of protein purification was analyzed by SDS-PAGE. In panel A) is shown the analysis of 519- His fusion protein purification. Mice were immunized with the purified 519-His and sera were used for Western blot (panel B), FACS analysis (panel C), bactericidal assay (panel D), and ELISA assay (panel E). Symbols: Ml, molecular weight marker; TP, N. meningitidis total protein extract; OMV, N. meningitidis outer membrane vesicle preparation. Arrows indicate the position of the main recombinant protein product (A) and the N. meningitidis immunoreactive band (B). These experiments confirm that 519 is a surface-exposed protein and that it is a useful immunogen. The hydrophilicity plots, antigenic index, and amphipatic regions of ORF 519 are provided in Figure 13. The AMPHI program is used to predict putative T-cell epitopes (Gao et al 1989, J. Immunol 143:3007; Roberts et al. 1996, AIDS Res Human Retroviruses 12:593 ; Quakyi et al. 1992, Scan d J Immunol Suppl 11 :9). The nucleic acid sequence of ORF 519 and the amino acid sequence encoded thereby is provided in Example 1. EXAMPLE 6 Expression of ORF 121 and 121-1 The primer described in Table 1 for ORF 121 was used to locate and clone ORF 121. The predicted gene 121 was cloned in pET vector and expressed in E. coli. The product of protein purification was analyzed by SDS-PAGE. In panel A) is shown the analysis of 121- His fusion protein purification. Mice were immunized with the purified 121 -His and sera were used for Western blot analysis (panel B), FACS analysis (panel C), bactericidal assay (panel D), and ELISA assay (panel E). Results show that 121 is a surface-exposed protein. Symbols: Ml, molecular weight marker; TP, N meningitidis total protein extract; OMV, N meningitidis outer membrane vescicle preparation. Arrows indicate the position of the main recombinant protein product (A) and the N meningitidis immunoreactive band (B). These experiments confirm that 121 is a surface-exposed protein and that it is a useful immunogen. The hydrophilicity plots, antigenic index, and amphipatic regions of ORF 121 are provided in Figure 14. The AMPHI program is used to predict putative T-cell epitopes (Gao et al 1989, J. Immunol 143:3007; Roberts et al. 1996, AIDS Res Human Retroviruses 12:593; Quakyi et al. 1992, Scand J Immunol Suppl 11:9). The nucleic acid sequence of ORF 121 and the amino acid sequence encoded thereby is provided in Example 1. EXAMPLE 7 Expression of ORF 128 and 128-1 The primer described in Table 1 for ORF 128 was used to locate and clone ORF 128. The predicted gene 128 was cloned in pET vector and expressed in E. coli. The product of protein purification was analyzed by SDS-PAGE. In panel A) is shown the analysis of 128- His purification. Mice were immunized with the purified 128-His and sera were used for Western blot analysis (panel B), FACS analysis (panel C), bactericidal assay (panel D) and ELISA assay (panel E). Results show that 128 is a surface-exposed protein. Symbols: Ml, molecular weight marker; TP, N meningitidis total protein extract; OMV, N meningitidis outer membrane vesicle preparation. Arrows indicate the position of the main recombinant protein product (A) and the N meningitidis immunoreactive band (B). These experiments confirm that 128 is a surface-exposed protein and that it is a useful immunogen. The hydrophilicity plots, antigenic index, and amphipatic regions of ORF 128 are provided in Figure 15. The AMPHI program is used to predict putative T-cell epitopes (Gao et al 1989, J. Immunol 143:3007; Roberts et al. 1996, AIDS Res Human Retroviruses 12:593; Quakyi et al. 1992, Scand J Immunol Suppl 11:9). The nucleic acid sequence of ORF 128 and the amino acid sequence encoded thereby is provided in Example 1. EXAMPLE 8 Expression of ORF 206 The primer described in Table 1 for ORF 206 was used to locate and clone ORF 206. The predicted gene 206 was cloned in pET vector and expressed in E. coli. The product of protein purification was analyzed by SDS-PAGE. In panel A) is shown the analysis of 206- His purification. Mice were immunized with the purified 206-His and sera were used for Western blot analysis (panel B). It is worthnoting that the immunoreactive band in protein extracts from meningococcus is 38 kDa instead of 17 kDa (panel A). To gain information on the nature of this antibody staining we expressed ORF 206 in E. coli without the His-tag and including the predicted leader peptide. Western blot analysis on total protein extracts from E. coli expressing this native form of the 206 protein showed a recative band at a position of 38 kDa, as observed in meningococcus. We conclude that the 38 kDa band in panel B) is specific and that anti-206 antibodies, likely recognize a multimeric protein complex. In panel C is shown the FACS analysis, in panel D the bactericidal assay, and in panel E) the ELISA assay. Results show that 206 is a surface-exposed protein. Symbols: Ml, molecular weight marker; TP, N meningitidis total protein extract; OMV, N. meningitidis outer membrane vesicle preparation. Arrows indicate the position of the main recombinant protein product (A) and the N meningitidis immunoreactive band (B). These experiments confirm that 206 is a surface- exposed protein and that it is a useful immunogen. The hydrophilicity plots, antigenic index, and amphipatic regions of ORF 519 are provided in Figure 16. The AMPHI program is used to predict putative T-cell epitopes (Gao et al 1989, J. Immunol 143:3007; Roberts et al. 1996, AIDS Res Human Retroviruses 12:593; Quakyi et al. 1992, Scand J Immunol Suppl 11:9). The nucleic acid sequence of ORF 206 and the amino acid sequence encoded thereby is provided in Example 1. EXAMPLE 9 Expression of ORF 287 The primer described in Table 1 for ORF 287 was used to locate and clone ORF 287. The predicted gene 287 was cloned in pGex vector and expressed in E. coli. The product of protein purification was analyzed by SDS-PAGE. In panel A) is shown the analysis of 287- GST fusion protein purification. Mice were immunized with the purified 287-GST and sera were used for FACS analysis (panel B), bactericidal assay (panel C), and ELISA assay (panel D). Results show that 287 is a surface-exposed protein. Symbols: Ml, molecular weight marker. Arrow indicates the position of the main recombinant protein product (A). These experiments confirm that 287 is a surface-exposed protein and that it is a useful immunogen. The hydrophilicity plots, antigenic index, and amphipatic regions of ORF 287 are provided in Figure 17. The AMPHI program is used to predict putative T-cell epitopes (Gao et al 1989, J. Immunol 143:3007; Roberts et al. 1996, AIDS Res Human Retroviruses 12:593; Quakyi et al. 1992, Scand J Immunol Suppl 11:9). The nucleic acid sequence of ORF 287 and the amino acid sequence encoded thereby is provided in Example 1. EXAMPLE 10 Expression of ORF 406 The primer described in Table 1 for ORF 406 was used to locate and clone ORF 406. The predicted gene 406 was cloned in pET vector and expressed in E. coli. The product of protein purification was analyzed by SDS-PAGE. In panel A) is shown the analysis of 406- His fusion protein purification. Mice were immunized with the purified 406-His and sera were used for Western blot analysis (panel B), FACS analysis (panel C), bactericidal assay (panel D), and ELISA assay (panel E). Results show that 406 is a surface-exposed protein. Symbols: Ml, molecular weight marker; TP, N. meningitidis total protein extract; OMV, N meningitidis outer membrane vescicle preparation. Arrows indicate the position of the main recombinant protein product (A) and the N meningitidis immunoreactive band (B). These experiments confirm that 406 is a surface-exposed protein and that it is a useful immunogen. The hydrophilicity plots, antigenic index, and amphipatic regions of ORF 406 are provided in Figure 18. The AMPHI program is used to predict putative T-cell epitopes (Gao et al 1989, J. Immunol 143:3007; Roberts et al. 1996, AIDS Res Human Retroviruses 12:593; Quakyi et al. 1992, Scand J Immunol Suppl 11:9). The nucleic acid sequence of ORF 406 and the amino acid sequence encoded thereby is provided in Example 1. EXAMPLE 11 Table 2 lists several Neisseria strains which were used to assess the conservation of the sequence of ORF 225 among different strains. Table 2 225 gene variability : List of used Neisseria strains Identification Strains Source / reference number Group B zo01_225 NG6/88 R. Moxon / Seiler et al, 1996 zo02 225 BZ198 R. Moxon / Seiler et al. 1996 zo03 225 NG3/88 R. Moxon / Seiler et al. 1996 zo04 225 297-0 R. Moxon / Seiler et al. 1996 zo05_225 1000 R. Moxon / Seiler et al. 1996 zo06 225 BZ147 R. Moxon / Seiler et al. 1996 zo07_225 BZ169 R. Moxon / Seiler et al. 1996 zo08 225 528 R. Moxon / Seiler et al. 1996 zo09 225 NGP165 R. Moxon / Seiler et al. 1996 zolO 225 BZ133 R. Moxon / Seiler et al. 1996 zol l 225 NGE31 R. Moxon / Seiler et al. 1996 zol2 225 NGF26 R. Moxon / Seiler et al. 1996 zol3 225 NGE28 R. Moxon / Seiler et al 1996 zol4 225 NGH38 R. Moxon / Seiler et al. 1996 zol5 225 SWZ107 R. Moxon / Seiler et al. 1996 zolό 225 NGH15 R. Moxon / Seiler et al. 1996 zol7 225 NGH36 R. Moxon / Seiler et al. 1996 zol8 225 BZ232 R. Moxon / Seiler et al. 1996 zol9_225 BZ83 R. Moxon / Seiler et al. 1996 zo20 225 44/76 R. Moxon / Seiler et al. 1996 zo21 225 MC58 R. Moxon zo96_225 2996 Our collection Group A zo22 225 205900 R. Moxon zo23 225 F6124 R. Moxon z2491 Z2491 R. Moxon / Maiden et al, 1998 Group C zo24 225 90/18311 R. Moxon zo25_225 93/4286 R. Moxon Others zo26 _225 A22 (group W) R. Moxon / Maiden et al, 1998 zo27 225 E26 (group X) R. Moxon / Maiden et al, 1998 zo28 ~ _225 860800 (group Y) R. Moxon / Maiden et al, 1998 zo29_ 225 E32 (group Z) R. Moxon / Maiden et al, 1998 Gonococcus zo32 225 Ng F62 R. Moxon / Maiden et al, 1998 zo33 " 225 Ng SN4 R. Moxon fal090 FA1090 R. Moxon References: Seiler A. et al, Mol. Microbiol, 1996, 19(4):841-856. Maiden et al., Proc. Natl. Acad. Sci. USA, 1998, 95:3140-3145. The amino acid sequences for each listed strain are as follows: >FA1090 <SEQ ID 3115> MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPVNRAPARRAG NADELIGSAMGLNEQPVLPVNRAPARRAGNADELIGSAMGLLGIAYRYGGTSVSTGFDCS GFMQHIFKRAMGINLPRTΞAEQARMGAPVARSELQPGDMVFFRTLGGSRISHVGLYIGNN RFIHAPRTGKNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN* Z2491 <SEQ ID 3116> MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLNEQPVLPVNRVPARRAGNA DELIGNAMGLNEQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSISTGFDCSGF MQHIFKRAMGINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRF IHAPRTGKNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN* ZO01_225 <SEQ ID 3117> MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNA DELIGNAMGLLGIAYRYGGTSISTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVAR SELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR VKKNDPSRFLN* ZO02_225 <SEQ ID 3118> MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNA DELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVAR SELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR VKKNDPSRFLN* ZO03_225 <SEQ ID 3119> MDSFFKPAVWAVLWLMFAVRLALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNA DELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVAR SELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR VKKNDPSRFLN* ZO04_225 <SEQ ID 3120> MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNA DELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVAR SELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR VKKNDPSRFLN* ZO05_225 <SEQ ID 3121> MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGSAMGLNEQPVLPVNRAPARRAGNA DELIGNAMGLLGIAYRYGGTSISTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVAR SELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR VKKNDPSRFLN* ZO06_225 <SEQ ID 3122> MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNA DELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVAR SELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR VKKNDPSRFLN* ZO07_225 <SEQ ID 3123> MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNA DELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVAR SELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR VKKNDPSRFLN* ZO08_225 <SEQ ID 3124> MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGΞAMGLNEQPVLPVNRAPARRAGNA DELIGNAMGLLGIAYRYGGTSISTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVAR SELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR VKKNDPSRFLN* ZO09_225 <SEQ ID 3125> MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNA DELIGNAMGLLGIAYRYGGTSISTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVAR SELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR VKKNDPSRFLN* ZO10_225 <SEQ ID 3126> MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNA DELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVAR SELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR VKKNDPSRFLN* ZOll_225 <SEQ ID 3127> MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNA DELIGNAMGLNEQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSVSTGFDCSGF MQHIFKRAMGINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRF IHAPRTGKNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN* Z012_225 <SEQ ID 3128> MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNA DELIGNAMGLLGIAYRYGGTSISTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVAR SELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR VKKNDPSRFLN* Z013 225 <SEQ ID 3129> MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPVNRAPARRAG NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNA DELIGNAMGLLGIAYRYGGTSVSTGFDCSGFIQHIFKRAMGINLPRTSAEQARMGTPVAR SELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR VKKNDPSRFLN* Z014_225 <ΞEQ ID 3130> MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNA DELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVAR SELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR VKKNDPSRFLN* Z015_225 <SEQ ID 3131> MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLLGIAYRYGGTSVSTGFDCS GFMQHIFKRAMGINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNN RFIHAPRTGKNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN* Z016_225 <SEQ ID 3132> MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNA DELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVAR SELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR VKKNDPSRFLN* Z017_225 <SEQ ID 3133> MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNA DELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVAR SELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR VKKNDPSRFLN* Z018_225 <SEQ ID 3134> MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNA DELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVAR SELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR VKKNDPSRFLN* Z019_225 <ΞEQ ID 3135> MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNA DELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVAR SELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR VKKNDPSRFLN* ZO20_225 <SEQ ID 3136> MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG NADELIGSAMGLNEQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNA DELIGNAMGLNEQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSVSTGFDCSGF MQHIFKRAMGINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRF IHAPRTGKNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN* Z021_225 <SEQ ID 3137> MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNA DELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVAR SELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR VKKNDPSRFLN* Z022_225 <SEQ ID 3138> MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNA DELIGNAMGLLGIAYRYGGTSISTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVAR SELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR VKKNDPSRFLN* Z023_225 <SEQ ID 3139> MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNA DELIGNAMGLLGIAYRYGGTSISTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVAR SELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR VKKNDPSRFLN* Z024_225 <SEQ ID 3140> MDΞFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNA DELIGNAMGLLGIAYRYGGTSISTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVAR SELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR VKKNDPSRFLN* Z025_225 <SEQ ID 3141> MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNA DELIGNAMGLLGIAYRYGGTSISTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVAR SELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR VKKNDPSRFLN* Z026_225 <SEQ ID 3142> MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNA DELIGNAMGLLGIAYRYGGTSISTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVAR SELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR VKKNDPSRFLN* Z027_225 <SEQ ID 3143> MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNA DELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVAR ΞELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR VKKNDPSRFLN* Z028_225 <SEQ ID 3144> MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNA DELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVAR SELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR VKKNDPSRFLN* Z029_225 <SEQ ID 3145> MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNA DELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVAR SELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR VKKNDPSRFLN* Z032_225 <SEQ ID 3146> MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPVNRAPARRAG NADELIGSAMGLNEQPVLPVNRAPARRAGNADELIGSAMGLLGIAYRYGGTSVSTGFDCS GFMQHIFKRAMGINLPRTSAEQARMGAPVARSELQPGDMVFFRTLGGSRISHVGLYIGNN RFIHAPRTGKNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN* Z033_225 <SEQ ID 3147> MDSFFKPAVWAVLWLMFAVRSALADELTNLLSSREQILRQFAEDEQPVLPVNRAPARRAG NADELIGSAMGLNEQPVLPVNRAPARRAGNADELIGSAMGLLGIAYRYGGTSVSTGFDCS GFMQHIFKRAMGINLPRTSAEQARMGAPVARSELQPGDMVFFRTLGGSRISHVGLYIGNN RFIHAPRTGKNIEITSLSHKYWSGKYAFARRIKKNDPSRFLN* Z096 225 <SEQ ID 3148> MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNA DELIGNAMGLLGIAYRYGGTSISTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVAR SELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR VKKNDPSRFLN* Figure 19 shows the results of aligning the sequences of each of these strains. Dark shading indicates regions of homology, and gray shading indicates the conservation of amino acids with similar characteristics. As is readily discernible, there is significant conservation among the various strains of ORF 225, further confirming its utility as an antigen for both vaccines and diagnostics. EXAMPLE 12 Table 3 lists several Neisseria strains which were used to assess the conservation of the sequence of ORF 235 among different strains. Table 3 235 gene ϊ variability : List of used Neisseria strains Identification Strains Reference number Group B gnmzqOl NG6/88 Seiler et al, 1996 gnmzq02 BZ198 Seiler et al, 1996 gnmzq03 NG3/88 Seiler et al, 1996 gnmzq04 1000 Seiler et al, 1996 gnmzq05 1000 Seiler et al, 1996 gnmzq07 BZ169 Seiler et al, 1996 gn zqOδ 528 Seiler et al, 1996 gnmzq09 NGP165 Seiler et al, 1996 gnmzqlO BZ133 Seiler et al, 1996 gnmzql l NGE31 Seiler et al, 1996 gnmzql3 NGE28 Seiler et al, 1996 gnmzql4 NGH38 Seiler et al, 1996 gnmzql5 SWZ107 Seiler et al, 1996 gnmzqlό NGH15 Seiler et al, 1996 gnmzql7 NGH36 Seiler et al, 1996 gnmzqlδ BZ232 Seiler et al, 1996 gnmzql9 BZ83 Seiler et al, 1996 gnmzq21 MC58 Virji t α/., 1992 Group A gnmzq22 205900 Our collection gnmzq23 F6124 Our collection z2491 Z2491 Maiden et al, 1998 Group C gnmzq24 90/18311 Our collection gnmzq25 93/4286 Our collection Others gnmzq26 A22 (group W) Maiden et al, 1998 gnmzq27 E26 (group X) Maiden et al, 1998 gnmzq28 860800 (group Y) Maiden et al, 1998 gnmzq29 E32 (group Z) Maiden et al, 1998 gnmzq31 N lactamica Our collection Gonococcus gnmzq32 Νg F62 Maiden et al, 1998 gnmzq33 Νg SΝ4 Our collection fal090 FA1090 Dempsey et al. 1991 References: Seiler A. et al, Mol. Microbiol., 1996, 19(4):841-856. Maiden R. et al, Proc. Natl. Acad. Sci. USA, 1998, 95:3140-3145. Viη ' i M. et al, Mol. Microbiol., 1992, 6:1271-1279 Dempsey J.F. et al, J. Bacteriol., 1991, 173:5476-5486 The amino acid sequences for each listed strain are as follows: FA1090 <SEQ ID 3149> MKPLILGLAAVLALSACQVRKAPDLDYTSFKESKPASILWPPLNESPDVNGTWGMLAST AAPISEAGYYVFPAAWEETFKENGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTS YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNSGLLGALVGAVVNQIANSLT DRGYQVSKTAAYNLLSPYSRNGILKGPRFVEEQPK* GNMZQ01 <SEQ ID 3150> MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILWPPLNESPDVNGTWGVLAST AAPLΞEAGYYVFPAAWEETFKENGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTS YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNSGLLGALVSAWNQIANNLT DRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK* GNMZQ02 <SEQ ID 3151> MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILWPPLNESPDVNGTWGVLAST AAPLSEAGYYVFPAAWEETFKQNGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTS YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNΞGLLGALVSAVVNQIANSLT DRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK* GNMZQ03 <SEQ ID 3152> MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILWPPLNESPDVNGTWGVLAST AAPLSEAGYYVFPAAWEETFKQNGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTS YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNSGLLGALVSAVVNQIANSLT DRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK* GNMZQ04 <SEQ ID 3153> MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILWPPLNESPDVNGTWGVLAST AAPLSEAGYYVFPAAWEETFKQNGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTS YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNSGLLGALVSAWNQIANSLT DRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK* GNMZQ05 <SEQ ID 3154> MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILWPPLNESPDVNGTWGVLAST AAPLSEAGYYVFPAAWEETFKENGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTS YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNSGLLGALVSAWNQIANNLT DRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK* GNMZQ07 <SEQ ID 3155> MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILWPPLNESPDVNGTWGVLAST AAPLSEAGYYVFPAAWEETFKQNGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTS YQILDSVTTVSAKARLVDSRNGKELWSGΞASIREGSNNSNSGLLGALVSAWNQIANSLT DRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK* GNMZQ08 <SEQ ID 3156> MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILWPPLNESPDVNGTWGVLAST AAPLSEAGYYVFPAAWEETFKENGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTS YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNSGLLGALVSAWNQIANNLT DRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK* GNMZQ09 <SEQ ID 3157> MKPLILGLAAALVLSACQVQKAPDFDYTSFKESKPASILWPPLNESPDVNGTWGMLAST AEPLSEAGYYVFPAAWEETFKQNGLTNAADIHAVQPEKLHQIFGNDAVLYITITEYGTS YQILDSVTTVSARARLVDSRNGKVLWSGSASIREGSNNSNSGLLGALVSAWNQIANSLT DRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK* GNMZQ10 <SEQ ID 3158> MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILWPPLNESPDVNGTWGVLAST AAPLSEAGYYVFPAAWEETFKQNGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTS YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNSGLLGALVSAWNQIANSLT DRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK* GNMZQ11 <SEQ ID 3159> MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILWPPLNESPDVNGTWGVLAST AAPLSEAGYYVFPAAWEETFKQNGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTS YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNSGLLGALVSAWNQIANSLT DRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK* GNMZQ13 <SEQ ID 3160> MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILWPPLNESPDVNGTWGVLAST AAPLSEAGYYVFPAAWEETFKQNGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTS YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNSGLLGALVSAWNQIANSLT DRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK* GNMZQ14 <SEQ ID 3161> MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILWPPLNESPDVNGTWGVLAST AAPLSEAGYYVFPAAWEETFKQNGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTS YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNSGLLGALVGAWNQIANSLT DRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK* GNMZQ15 <SEQ ID 3162> MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILWPPLNESPDVNGTWGVLAST AAPLSEAGYYVFPAAWEETFKQNGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTS YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNSGLLGALVSAWNQIANSLT DRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK* GNMZQ16 <SEQ ID 3163> MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILWPPLNESPDVNGTWGVLAST AAPLSEAGYYVFPAAWEETFKQNGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTS YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNSGLLGALVSAWNQIANSLT DRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK* GNMZQ17 <SEQ ID 3164> MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILWPPLNESPDVNGTWGVLAST AAPLSEAGYYVFPAAWEETFKQNGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTS YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNSGLLGALVSAWNQIANSLT DRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK* GNMZQ18 <SEQ ID 3165> MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILWPPLNESPDVNGTWGVLAST AAPLSEAGYYVFPAAWEETFKQNGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTS YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNSGLLGALVGAWNQIANSLT DRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK* GNMZQ19 <SEQ ID 3166> MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILWPPLNESPDVNGTWGVLAST AAPLSEAGYYVFPAAWEETFKQNGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTS YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNSGLLGALVSAWNQIANSLT DRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK* GNMZQ21 <SEQ ID 3166> MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPLNESPDVNGTWGVLAST AAPLSEAGYYVFPAAWEETFKQNGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTS YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNSGLLGALVSAWNQIANSLT DRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK* GNMZQ22 <SEQ ID 3167> MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILWPPLNESPDVNGTWGVLAST AAPLSEAGYYVFPAAWEETFKQNGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTS YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNΞGLLGALVSAWNQIANSLT DRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK* GNMZQ23 <SEQ ID 3168> MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILWPPLNESPDVNGTWGVLAST AAPLSEAGYYVFPAAWEETFKQNGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTS YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNSGLLGALVSAWNQIANSLT DRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK* GNMZQ24 <SEQ ID 3169> MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILWPPLNESPDVNGTWGVLAST AAPLSEAGYYVFPAAWEETFKQNGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTS YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNSGLLGALVSAWNQIANSLT DRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK* GNMZQ25 <SEQ ID 3170> MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILWPPLNESPDVNGTWGVLAST AAPLSEAGYYVFPAAWEETFKQNGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTS YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNSGLLGALVSAWNQIANSLT DRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK* GNMZQ26 <SEQ ID 3171> MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILWPPLNESPDVNGTWGMLAST AAPLSEAGYYVFPAAWEETFKQNGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTS YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNSGLLGALVGAWNQIANSLT DRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK* GNMZQ27 <SEQ ID 3172> MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILWPPLNESPDVNGTWGVLAST AAPLSEAGYYVFPAAWEETFKQNGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTS YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNSGLLGALVSAWNQIANSLT DRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK* GNMZQ28 <SEQ ID 3173> MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPLNESPDVNGTWGVLAST AAPLSEAGYYVFPAAWEETFKQNGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTS YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNSGLLGALVSAWNQIANSLT DRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK* GNMZQ29 <SEQ ID 3174> MKPLI LGLAAVLALSACQVQKAPDFDYTS FKE SKPAS I LWPPLNE S PDVNGTWGVLAST AAPLSEAGYYVFPAAWEETFKQNGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTS YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNSGLLGALVSAWNQIANSLT DRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK* GNMZQ31 <SEQ ID 3175> MKPLILGLAAVLALSACQVQKAPDFDYTAFKESKPASILWPPLNESPDVNGTWGMLAST AEPLSEAGYYVFPAAWEETFKQNGLTNAADIHAVRPEKLHQIFGNDAVLYITITEYGTS YQILDSVTTVSARARLVDSRNGKVLWSGSASIREGSNNSNSGLLGALVGAWNQIANSLT DRGYQVSKAAAYDLLSPYSHNGILKGPRFVEEQPK* GNMZQ32 <SEQ ID 3176> MKPLILGLAAVLALSACQVRKAPDLDYTSFKESKPASILVVPPLNEΞPDVNGTWGMLAST AAPISEAGYYVFPAAWEETFKENGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTS YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNSGLLGALVGAWNQIANSLT DRGYQVΞKTAAYNLLSPYSRNGILKGPRFVEEQPK* GNMZQ33 <SEQ ID 3177> MKPLILGLAAVLALSACQVRKAPDLDYTSFKESKPASILWPPLNESPDVNGTWGMLAST AAPISEAGYYVFPAAWEETFKENGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTS YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNSGLLGALVGAWNQIANSLT DRGYQVSKTAAYNLLSPYSRNGILKGPRFVEEQPK* Z2491 <SEQ ID 3178> MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILWPPLNESPDVNGTWGVLAST AAPLSEAGYYVFPAAWEETFKQNGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTS YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNSGLLGALVSAWNQIANSLT DRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK* Figure 20 shows the results of aligning the sequences of each of these strains. Dark shading indicates regions of homology, and gray shading indicates the conservation of amino acids with similar characteristics. As is readily discernible, there is significant conservation among the various strains of ORF 235, further confirming its utility as an antigen for both vaccines and diagnostics. EXAMPLE 13 Table 4 lists several Neisseria strains which were used to assess the conservation of the sequence of ORF 287 among different strains. Table 4 287 gene variability: List of used Neisseria strains Identification Strains Reference number Group B 287 2 BZ198 Seiler et al, 1996 287 9 NGP165 Seiler et al, 1996 287 14 NGH38 Seiler et al, 1996 287_21 MC58 Virji et α/., 1992 Group A z2491 Z2491 Maiden et al, 1998 Gonococcus fal090 FA1090 Dempsey et al. 1991 References: Seiler A. etal, Mol. Microbiol., 1996, 19(4):841-856. Maiden R. et al, Proc. Natl. Acad. Sci. USA, 1998, 95:3140-3145. Virji M. et al, Mol. Microbiol, 1992, 6:1271-1279 Dempsey J.F. et al, J. Bacteriol., 1991, 173:5476-5486 The amino acid sequences for each listed strain are as follows: 287_14 <SEQ ID 3179> MFKRSVIAMACIFALSACGGGGGGSPDVKSADTLΞKPAAPWSEKETEAKEDAPQAGSQG QGAPSAQGGQDMAAVSEENTGNGGAAATDKPKNEDEGAQNDMPQNAADTDSLTPNHTPAS NMPAGNMENQAPDAGESEQPANQPDMANTADGMQGDDPSAGGENAGNTAAQGTNQAENNQ TAGSQNPASSTNPSATNSGGDFGRTNVGNSWIDGPSQNITLTHCKGDSCSGNNFLDEEV QLKSEFEKLSDADKISNYKKDGKNDGKNDKFVGLVADSVQMKGINQYIIFYKPKPTSFAR FRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGNIFAPEGNYRYLTYGAEKLP GGSYALRVQGEPSKGEMLAGTAVYNGEVLHFHTENGRPSPSRGRFAAKVDFGSKSVDGII DSGDGLHMGTQKFKAAIDGNGFKGTWTENGGGDVSGKFYGPAGEEVAGKYSYRPTDAEKG GFGVFAGKKEQD* 287_2 <SEQ ID 3180> MFKRSVIAMACIFALSACGGGGGGSPDVKSADTLSKPAAPWSEKETEAKEDAPQAGSQG QGAPSAQGGQDMAAVSEENTGNGGAAATDKPKNEDEGAQNDMPQNAADTDSLTPNHTPAS NMPAGNMENQAPDAGESEQPANQPDMANTADGMQGDDPSAGGENAGNTAAQGTNQAENNQ TAGSQNPASSTNPSATNSGGDFGRTNVGNSWIDGPSQNITLTHCKGDSCSGNNFLDEEV QLKSEFEKLSDADKISNYKKDGKNDGKNDKFVGLVADSVQMKGINQYIIFYKPKPTSFAR FRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGNIFAPEGNYRYLTYGAEKLP GGSYALRVQGEPSKGEMLAGTAVYNGEVLHFHTENGRPSPSRGRFAAKVDFGSKSVDGII DSGDGLHMGTQKFKAAIDGNGFKGTWTENGGGDVSGKFYGPAGEEVAGKYSYRPTDAEKG GFGVFAGKKEQD* 287_21. <SEQ ID 3181> MFKRSVIAMACIFALSACGGGGGGSPDVKSADTLSKPAAPWSEKETEAKEDAPQAGSQG QGAPSAQGSQDMAAVSEENTGNGGAVTADNPKNEDEVAQNDMPQNAAGTDSSTPNHTPDP NMLAGNMENQATDAGESSQPANQPDMANAADGMQGDDPSAGGQNAGNTAAQGANQAGNNQ AAGSSDPIPASNPAPANGGSNFGRVDLANGVLIDGPSQNITLTHCKGDSCSGNNFLDEEV QLKSEFEKLSDADKISNYKKDGKNDKFVGLVADSVQMKGINQYIIFYKPKPTSFARFRRS ARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGNIFAPEGNYRYLTYGAEKLPGGSY ALRVQGEPAKGEMLAGAAVYNGEVLHFHTENGRPYPTRGRFAAKVDFGSKSVDGIIDSGD DLHMGTQKFKAAIDGNGFKGTWTENGSGDVSGKFYGPAGEEVAGKYSYRPTDAEKGGFGV FAGKKEQD* 287_9 <SEQ ID 3182> MFKRSVIAMACIVALSACGGGGGGSPDVKSADTLSKPAAPWTEDVGEEVLPKEKKDEEA VSGAPQADTQDATAGKGGQDMAAVSAENTGNGGAATTDNPENKDEGPQNDMPQNAADTDS STPNHTPAPNMPTRDMGNQAPDAGESAQPANQPDMANAADGMQGDDPSAGENAGNTADQA ANQAENNQVGGSQNPASSTNPNATNGGSDFGRINVANGIKLDSGSENVTLTHCKDKVCDR DFLDEEAPPKSEFEKLSDEEKINKYKKDEQRENFVGLVADRVEKNGTNKYVIIYKDKSAS SSSARFRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGNIFAPEGNYRYLTYG AEKLSGGSYALSVQGEPAKGEMLAGTAVYNGEVLHFHMENGRPSPSGGRFAAKVDFGSKS VDGIIDSGDDLHMGTQKFKAVIDGNGFKGTWTENGGGDVSGRFYGPAGEEVAGKYSYRPT DAEKGGFGVFAGKKEQD* FA1090 <SEQ ID 3183> MFKRSVIAMACIFPLSACGGGGGGSPDVKSADTPSKPAAPWAENAGEGVLPKEKKDEEA AGGAPQADTQDATAGEGSQDMAAVSAENTGNGGAATTDNPKNEDAGAQNDMPQNAAESAN QTGNNQPAGSSDSAPASNPAPANGGSDFGRTNVGNSWIDGPSQNITLTHCKGDSCNGDN LLDEEAPSKSEFEKLSDEEKIKRYKKDEQRENFVGLVADRVKKDGTNKYIIFYTDKPPTR SARSRRSLPAEIPLIPVNQADTLIVDGEAVSLTGHSGNIFAPEGNYRYLTYGAEKLPGGS YALRVQGEPAKGEMLVGTAVYNGEVLHFHMENGRPYPSGGRFAAKVDFGSKSVDGIIDSG DDLHMGTQKFKAAIDGNGFKGTWTENGGGDVSGRFYGPAGEEVAGKYSYRPTDAEKGGFG VFAGKKDRD* Z2491 <SEQ ID 3184> MFKRSVIAMACIFALSACGGGGGGSPDVKSADTLSKPAAPWSEKETEAKEDAPQAGSQG QGAPSAQGSQDMAAVSEENTGNGGAVTADNPKNEDEVAQNDMPQNAAGTDSSTPNHTPDP NMLAGNMENQATDAGESSQPANQPDMANAADGMQGDDPSAGGQNAGNTAAQGANQAGNNQ AAGSSDPIPASNPAPANGGSNFGRVDLANGVLIDGPSQNITLTHCKGDSCSGNNFLDEEV QLKSEFEKLSDADKISNYKKDGKNDKFVGLVADSVQMKGINQYIIFYKPKPTSFARFRRS ARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGNIFAPEGNYRYLTYGAEKLPGGSY ALRVQGEPAKGEMLAGAAVYNGEVLHFHTENGRPYPTRGRFAAKVDFGSKSVDGIIDSGD DLHMGTQKFKAAIDGNGFKGTWTENGSGDVSGKFYGPAGEEVAGKYSYRPTDAEKGGFGV FAGKKEQD* Figure 21 shows the results of aligning the sequences of each of these strains. Dark shading indicates regions of homology, and gray shading indicates the conservation of amino acids with similar characteristics. As is readily discernible, there is significant conservation among the various strains of ORF 287, further confirming its utility as an antigen for both vaccines and diagnostics. EXAMPLE 14 Table 5 lists several Neisseria strains which were used to assess the conservation of the sequence of ORF 519 among different strains. Table 5 519 gene variability: List of used Neisseria strains Identification Strains Source / reference number Group B zvOl 519 NG6/88 R. Moxon / Seiler et al, 1996 zv02_519 BZ198 R. Moxon / Seiler et al, 1996 zv03 519ass NG3/88 R. Moxon / Seiler et al, 1996 zv04 519 297-0 R. Moxon / Seiler et al, 1996 zv05_519 1000 R. Moxon / Seiler et al, 1996 zv06 519ass BZ147 R. Moxon / Seiler et al, 1996 zv07 519 BZ169 R. Moxon / Seiler et al, 1996 zvl l 519 NGE31 R. Moxon / Seiler et al, 1996 zvl2 519 NGF26 R. Moxon / Seiler et al, 1996 zvl8 519 BZ232 R. Moxon / Seiler et al, 1996 zvl9_519 BZ83 R. Moxon / Seiler et al, 1996 zv20_519ass 44/76 R. Moxon / Seiler et al, 1996 zv21 519ass MC58 R. Moxon zv96_519 2996 Our collection Group A zv22 519ass 205900 R. Moxon z2491_519 Z2491 R. Moxon / Maiden et al, 1998 Others zv26 519 A22 (group W) R. Moxon / Maiden et al, 1998 zv27 519 E26 (group X) R. Moxon / Maiden et al, 1998 zv28_519 860800 (group Y) R. Moxon / Maiden et al, 1998 zv29_519ass E32 (group Z) R. Moxon / Maiden et al, 1998 Gonococcus zv32_519 Ng F62 R Moxon / Maiden et al, 1998 fal090_519 FA1090 R. Moxon References: Seiler A. et al ., Mol. Microbiol., 1996, 19(4):841-856. Maiden et al., Proc. Natl. Acad. Sci. USA, 1998, 95:3140-3145. The amino acid sequences for each listed strain are as follows: FA1090_519 <SEQ ID 3185> MEFFIILLA AVAV FGFKSFWIP QQ E V H W ERLGRFHRALTAGLNILIPFIDRVAYRHSL KEIPLDVPS QVC ITRDNT Q LT V D G IIY FQV TDPKLASYGSSNYIMAITQLAQTTLRSVIG RMELDKTFEERDEIN S T W SALDEAAGA WGV KVLRYEIKDLVPPQEILRAMQAQITAERE KRARIAESEGRKIE Q I N LASG Q REAEI QQS EGEA Q AAVNASNAEKIARINRAKGEAESLR LVAEANAEAIR Q IAAAL Q T Q GGADA V NLKIAE Q YVAAFNNLAKESNTLIMPANVADIGSL ISAGMKIIDSSKTAK* Z2491_519 <SEQ ID 3186> MEFFIILLAAVVVFGFKSFWIPQQEVHWERLGRFHRALTAGLNILIPFIDRVAYRHSL KEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIG RMELDKTFEERDEINSTWSALDEAAGAWGVKVLRYEIKDLVPPQEILRSMQAQITAERE KRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL ISAGMKIIDSSKTAK* ZV01_519 <SEQ ID 3187> MEFFIILLVAVAVFGFKSFWIPQQEVHWERLGRFHRALTAGLNILIPFIDRVAYRHSL KEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIG RMELDKTFEERDEINSTWAALDEAAGAWGVKVLRYEIKDLVPPQEILRSMQAQITAERE KRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL ISAGMKIIDSSKTAK* ZV02_519 <SEQ ID 3188> MEFFIILLVAVAVFGFKSFWIPQQEVHWERLGRFHRALTAGLNILIPFIDRVAYRHSL KEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIG RMELDKTFEERDEINSTWSALDEAAGAWGVKVLRYEIKDLVPPQEILRSMQAQITAERE KRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL ISAGMKIIDSSKTAK* ZV03_519 <SEQ ID 3189> MEFFIILLVAVAVFGFKSFWIPQQEVHWERLGRFHRALTAGLNILIPFIDRVAYRHSL KEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIG RMELDKTFEERDEINSTWSALDEAAGAWGVKVLRYEIKDLVPPQEILRSMQAQITAERE KRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL ISAGMKIIDSSKTAK* ZV04_519 <SEQ ID 3190> MEFFIILLVAVAVFGFKSFWIPQQEVHWERLGRFHRALTAGLNILIPFIDRVAYRHSL KEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIG RMELDKTFEERDEINSTWSALDEAAGAWGVKVLRYEIKDLVPPQEILRSMQAQITAERE KRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL ISAGMKIIDSSKTAK* ZV05_519 <SEQ ID 3191> MEFFIILLVAVAVFGFKSFWIPQQEVHWERLGRFHRALTAGLNILIPFIDRVAYRHSL KEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIG RMELDKTFEERDEINSTWSALDEAAGAWGVKVLRYEIKDLVPPQEILRSMQAQITAERE KRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL ISAGMKIIDSSKTAK* ZV06_519ASS <SEQ ID 3192> MEFFIILLVAVAVFGFKSFWIPQQEVHWERLGRFHRALTAGLNILIPFIDRVAYRHSL KEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIG RMELDKTFEERDEINSTVFSALDEAAGAWGVKVLRYEIKDLVPPQEILRSMQAQITAERK KRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL ISAGMKIIDSSKTAK* ZV07_519 <SEQ ID 3193> MEFFIILLVAVAVFGFKSFVVIPQQEVHWERLGRFHRALTAGLNILIPFIDRVAYRHSL KEIPLDVPSQVCITRDNT Q LTVDGIIYFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIG RMELDKTFEERDEINSTWAALDEAAGAWGVKVLRYEIKDLVPPQEILRSMQAQITAERE KRARIAESEGRKIEQINLASG Q REAEIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL ISAGMKIIDSSKTAK* ZV11_519 <SEQ ID 3194> MEFFIILLAAVAVFGFKSFVVIPQQEVHWERLGRFHRALTAGLNILIPFIDRVAYRHSL KEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIG RMELDKTFEERDEINSTWAALDEAAGAWGVKVLRYEIKDLVPPQEILRΞMQAQITAERE KRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL ISAGMKIIDSSKTAK* ZV12_519 <SEQ ID 3195> MEFFIILLVAVAVFGFKSFWIPQQEVHWERLGRFHRALTAGLNILIPFIDRVAYRHSL KEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIG RMELDKTFEERDEINSTWAALDEAAGAWGVKVLRYEIKDLVPPQEILRSMQAQITAERE KRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL ISAGMKIIDSSKTAK* ZV18_519 <SEQ ID 3196> MEFFIILLVAVAVFGFKSFWIPQQEVHWERLGRFHRALTAGLNILIPFIDRVAYRHSL KEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIG RMELDKTFEERDEINSTWAALDEAAGAWGVKVLRYEIKDLVPPQEILRSMQAQITAERE KRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL ISAGMKIIDSSKTAK* ZV19_519 <SEQ ID 3197> MEFFIILLVAVAVFGFKSFWIPQQEVHWERLGRFHRALTAGLNILIPFIDRVAYRHSL KEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIG RMELDKTFEERDEINSTVVAALDEAAGAWGVKVLRYEIKDLVPPQEILRSMQAQITAERE KRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL ISAGMKIIDSSKTAK* ZV20_519ASS <SEQ ID 3198> MEFFIILLVAVAVFGFKSFWIPQQEVHWERLGRFHRALTAGLNILIPFIDRVAYRHSL KEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIG RMELDKTFEERDEINSTWAALDEAAGAWGVKVLRYEIKDLVPPQEILRSMQAQITAERE KRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSM ISAGMKIIDSSKTAK* ZV21_519ASS <SEQ ID 3199> MEFFIILLVAVAVFGFKSFWIPQQEVHWERLGRFHRALTAGLNILIPFIDRVAYRHSL KEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIG RMELDKTFEERDEINSTWAALDEAAGAWGVKVLRYEIKDLVPPQEILRSMQAQITAERE KRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL ISAGMKIIDSSKTAK* ZV22_519ASS <SEQ ID 3200> MEFFIILLAAVVVFGFKSFWIPQQEVHWERLGRFHRALTAGLNILIPFIDRVAYRHSL KEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIG RMELDKTFEERDEINSTWSALDEAAGAWGVKVLRYEIKDLVPPQEILRSMQAQITAERE KRARIAESEGRKIEQINLASGQREAKIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL ISAGMKIIDSSKTAK* ZV26_519 <SEQ ID 3201> MEFFIILLAAVWFGFKSFWIPQQEVHWERLGRFHRALTAGLNILIPFIDRVAYRHSL KEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIG RMELDKTFEERDEINSTVVAALDEAAGAWGVKVLRYEIKDLVPPQEILRSMQAQITAERE KRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL ISAGMKIIDSSKTAK* ZV27 519 <ΞEQ ID 3202> MEFFIILLVAVAVFGFKSFWIPQQEVHWERLGRFHRALTAGLNILIPFIDRVAYRHSL KEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIG RMELDKTFEERDEINSTWAALDEAAGAWGVKVLRYEIKDLVPPQEILRSMQAQITAERE KRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL ISAGMKIIDSSKTAK* ZV28_519 <SEQ ID 3203> MEFFIILLAAVAVFGFKSFWIPQQEVHWERLGRFHRALTAGLNILIPFIDRVAYRHSL KEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIG RMELDKTFEERDEINSTWAALDEAAGAWGVKVLRYEIKDLVPPQEILRSMQAQITAERE KRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL ISAGMKIIDSSKTAK* ZV29_519ASS <SEQ ID 3204> MEFFIILLAAVAVFGFKSFWIPQQEVHWERLGRFHRALTAGLNILIPFIDRVAYRHSL KEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIG RMELDKTFEERDEINSIWSALDEAAGAWGVKVLRYEIKDLVPPQEILRSMQAQITAERE KRARIAESEGRKIEQINLASGQREPEIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL ISAGMKIIDSNKTAK* ZV32_519 <SEQ ID 3205> MEFFIILLAAVAVFGFKSFWIPQQEVHWERLGRFHRALTAGLNILIPFIDRVAYRHSL KEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIG RMELDKTFEERDEINSTWSALDEAAGAWGVKVLRYEIKDLVPPQEILRAMQAQITAERE KRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL ISAGMKIIDSSKTAK* ZV96_519 <SEQ ID 3206> MEFFIILLAAVAVFGFKSFWIPQQEVHWERLGRFHRALTAGLNILIPFIDRVAYRHSL KEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIG RMELDKTFEERDEINSTWAALDEAAGAWGVKVLRYEIKDLVPPQEILRSMQAQITAERE KRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL ISAGMKIIDSSKTAK* Figure 22 shows the results of aligning the sequences of each of these strains. Dark shading indicates regions of homology, and gray shading indicates the conservation of amino acids with similar characteristics. As is readily discernible, there is significant conservation among the various strains of ORF 519, further confirming its utility as an antigen for both vaccines and diagnostics. EXAMPLE 15 Table 6 lists several Neisseria strains which were used to assess the conservation of the sequence of ORF 919 among different strains. Table 6 919 gene variability: List of used Neisseria strains Identification Strains Source / reference number Group B zmOl NG6/88 R. Moxon / Seiler et al, 1996 zm02 BZ198 R. Moxon / Seiler et al, 1996 zm03 NG3/88 R. Moxon / Seiler et al, 1996 zm04 297-0 R. Moxon / Seiler et al, 1996 zm05 1000 R. Moxon / Seiler et al, 1996 zm06 BZ147 R. Moxon / Seiler et al, 1996 zm07 BZ169 R. Moxon / Seiler et al, 1996 zm08n 528 R. Moxon / Seiler et al, 1996 zm09 NGP165 R. Moxon / Seiler et al, 1996 zmlO BZ133 R. Moxon / Seiler et al, 1996 zml lasbc NGE31 R. Moxon / Seiler et al, 1996 zml2 NGF26 R. Moxon / Seiler et al, 1996 zml3 NGE28 R. Moxon / Seiler et al, 1996 zml4 NGH38 R. Moxon / Seiler et al, 1996 ζml5 SWZ107 R. Moxon / Seiler et al, 1996 zmlό NGH15 R. Moxon / Seiler et al, 1996 zml7 NGH36 R. Moxon / Seiler et al, 1996 zml8 BZ232 R. Moxon / Seiler et al, 1996 zml9 BZ83 R. Moxon / Seiler et al, 1996 zm20 44/76 R. Moxon / Seiler et al, 1996 zm21 MC58 R. Moxon zm96 2996 Our collection Group A zm22 205900 R. Moxon zm23asbc F6124 R. Moxon z2491 Z2491 R. Moxon / Maiden et al, 1998 Group C zm24 90/18311 R. Moxon zm25 93/4286 R. Moxon Others zm26 A22 (group W) R. Moxon / Maiden et al, 1998 zm27bc E26 (group X) R. Moxon / Maiden et al, 1998 zm28 860800 (group Y) R. Moxon / Maiden et al, 1998 zm29asbc E32 (group Z) R. Moxon / Maiden et al, 1998 zm31asbc N. lactamica R. Moxon Gonococcus zm32asbc Ng F62 R. Moxon / Maiden et al, 1998 zm33asbc Ng SN4 R. Moxon fal090 FA1090 R. Moxon References: Seiler A. et al, Mol. Microbiol., 1996, 19(4):841-856. Maiden et al., Proc. Natl. Acad. Sci. USA, 1998, 95:3140-3145. The amino acid sequences for each listed strain are as follows: FA1090 <SEQ ID 3207> MKKHLLRSALYGIAAAILAACQSRSIQTFPQPDTSVINGPDRPAGIPDPAGTTVAGGGAV YTWPHLSMPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKRFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDGRRTERARFPIYGIPDDFISVPLPAGLRGGKN LVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL KLGQTSMQGIKAYMRQNPQRLAEVLGQNPSYIFFRELAGSGNEGPVGALGTPLMGEYAGA IDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* Z2491 <SEQ ID 3208> MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPDPAGTTVGGGGAV YTWPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSVQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNTGGTHTADLSQFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL KLGQTSMQGIKAYMQQNPQRLAEVLGQNPSYIFFRELTGSSNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* ZM01 <SEQ ID 3209> MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPDPAGTTVGGGGAV YTWPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL KLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFFRELAGSSNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* ZM02 <SEQ ID 3210> MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPDPAGTTVGGGGAV YTWPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL KLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFFRELAGSSNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* ZM03 <SEQ ID 3211> MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPDPAGTTVGGGGAV YTWPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHI Q GSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL KLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFFRELAGSSNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* ZM04 <SEQ ID 3212> MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPAPAGTTVAGGGAV YTWPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL KLGQTSMQGIKAYMQQNPQRLAEVLGQNPSYIFFRELTGSSNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* ZM05 <SEQ ID 3213> MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPDPAGTTVGGGGAV YTWPHLSLPHWAAQDFAKSLQSFRLSCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL KLGQTSMQGIKAYMRQNPQRLAEVLGQNPSYIFFRELAGSSNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* ZM06 <SEQ ID 3214> MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPDPAGTTVGGGGAV YTWPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGKYMADKGYL KLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFFRELAGSSNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* ZM07 <SEQ ID 3215> MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPDPAGTTVGGGGAV YTWPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL KLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFFRELAGSSNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* ZM08N <SEQ ID 3216> MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPDPAGTTVGGGGAV YTWPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL KLGQTSMQGIKAYMRQNPQRLAEVLGQNPSYIFFRELAGSSNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* ZM09 <SEQ ID 3217> MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPAPAGTTVAGGGAV YTWPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNTGGTHTADLSQFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGKYMADKGYL KLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFFRELTGSGNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* ZM10 <SEQ ID 3218> MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPAPAGTTVAGGGAV YTWPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNTGGTHTADLSQFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGKYMADKGYL KLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFFRELTGSGNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* ZM11ASBC <ΞEQ ID 3219> MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPAPAGTTVGGGGAV YTWPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSVQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGKYMADKGYL KLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFFRELTGSSNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* ZM12 <SEQ ID 3220> MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPDPAGTTVGGGGAV YTWPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL KLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFFRELAGSSNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* ZM13 <SEQ ID 3221> MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPDPAGTTVGGGGAV YTWPHLSLPHWAEQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL KLGQTSMQGIKAYMRQNPQRLAEVLGQNPSYIFFRELAGSSNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* ZM14 <SEQ ID 3222> MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPAPAGTTVAGGGAV YTWPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGKYMADKGYL KLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFFRELTGSRNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* ZM15 <SEQ ID 3223> MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPDLAGTTVGGGGAV YTWPHLSLPHWAAQDFAKSLQSFRLGCANLKNHQGWQDVCAQAFQTPVHSFQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGKYMADKGYL KLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFFRELTGSGNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* ZM16 <SEQ ID 3224> MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPGRPVGIPDPAGTTVGGGGAV YTWPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNTGGTHTADLSQFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGKYMADKGYL KLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFFRELTGSSNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* ZM17 <SEQ ID 3225> MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPDPAGTTVGGGGAV YTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGKYMADKGYL KLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFFRELTGSSNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* ZM18 <SEQ ID 3226> MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPDPAGTTVGGGGAV YTWPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL KLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFFRELAGSSNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* ZM19 <SEQ ID 3227> MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPDPAGTTVGGGGAV YTWPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL KLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFFRELAGSSNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* ZM20 <SEQ ID 3228> MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPDPAGTTVGGGGAV YTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL KLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFFRELAGSSNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* ZM21 <SEQ ID 3229> MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPDPAGTTVGGGGAV YTWPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL KLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFFRELAGSSNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* ZM22 <SEQ ID 3230> MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPDPAGTTVGGGGAV YTWPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSVQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNTGGTHTADLSQFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL KLGQTSMQGIKAYMQQNPQRLAEVLGQNPSYIFFRELTGSSNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* ZM23ASBC <SEQ ID 3231> MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPDPAGTTVGGGGAV YTWPHLΞLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGKYMADKGYL KLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFFRELAGSSNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATAHPVTSKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGETAGK MKEPGYVWQLLPNGMKPEYRP* ZM24 <SEQ ID 3232> MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPAPAGTTVAGGGAV YTWPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNTGGTHTADLSQFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGKYMADKGYL KLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFFRELTGSGNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* ZM25 <SEQ ID 3233> MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPAPAGTTVAGGGAV YTWPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNTGGTHTADLSQFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGKYMADKGYL KLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFFRELTGSGNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* ZM26 <SEQ ID 3234> MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPDPAGTTVGGGGAV YTWPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSVQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNTGGTHTADLSQFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL KLGQTSMQGIKAYMQQNPQRLAEVLGQNPSYIFFRELTGSSNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* ZM27BC <SEQ ID 3235> MKKYLFRAALYGISAAILAACQSKSIQTFPQPDTSVINGPDRPAGIPDPAGTTVAGGGAV YTWPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL KLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFFRELTGSSNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGETAGK MKEPGYVWQLLPNGMKPEYRP* ZM28 <SEQ ID 3236> MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPDPAGTTVGGGGAV YTWPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNTGGTHTADLSQFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL KLGQTSMQGIKAYMRQNPQRLAEVLGQNPSYIFFRELAGSSNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* ZM29ASBC <SEQ ID 3237> MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPDPAGTTVGGGGAV YTWPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNTGGTHTADLSQFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL KLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFFRELTGSGNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATTHPITRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* ZM31ASBC <SEQ ID 3238> MKKHLFRAALYGIAAAILAACQSKSIQTFPQPDTSIIKGPDRPAGIPDPAGTTVGGGGAV YTWPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL KLGQTSMQGIKAYMRQNPQRLAEVLGQNPSYVFFRELAGSGNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* ZM32ASBC <SEQ ID 3239> MKKHLLRSALYGIAAAILAACQSRSIQTFPQPDTSVINGPDRPAGIPDPAGTTVAGGGAV YTWPHLSMPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKRFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDGRRTERARFPIYGIPDDFISVPLPAGLRGGKA LVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL KLGQTSMQGIKAYMRQNPQRLAEVLGQNPSYIFFRELAGSGGDGPVGALGTPLMGGYAGA IDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* ZM33ASBC <SEQ ID 3240> MKKHLLRSALYGIAAAILAACQSRSIQTFPQPDTSVINGPDRPAGIPDPAGTTVAGGGAV YTWPHLSMPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPIHSFQAKRFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDGRRTERARFPIYGIPDDFISVPLPAGLRGGKN LVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL KLGQTSMQGIKSYMRQNPHKLAEVLGQNPSYIFFRELAGSGNEGPVGALGTPLMGEYAGA IDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* ZM96 <SEQ ID 3241> MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPDPAGTTVGGGGAV YTWPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA LVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL KLGQTSMQGIKAYMRQNPQRLAEVLGQNPSYIFFRELAGSSNDGPVGALGTPLMGEYAGA VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK QKTTGYVWQLLPNGMKPEYRP* Figure 23 shows the results of aligning the sequences of each of these strains. Dark shading indicates regions of homology, and gray shading indicates the conservation of amino acids with similar characteristics. As is readily discernible, there is significant conservation among the various strains of ORF 919, further confirming its utility as an antigen for both vaccines and diagnostics. EXAMPLE 16 Using the above-described procedures, the following oligonucleotide primers were employed in the polymerase chain reaction (PCR) assay in order to clone the ORFs as indicated: Table 7: Oligonucleotides used for PCR to amplify complete or partial ORFs ORF primer Sequence Restriction sites 001 Forward CGCGGATCCCATATG-TGGATGGTGCTGGTCAT BamHI- Ndel Reverse CCCGCTCGAG-TGCCGTCTTGTCCCAC Xhol 003 Forward CGCGGATCCCATATG-GTCGTATTCGTGGC BamHI- Ndel Reverse CCCGCTCGAG-AAAATCATGAACACGCGC Xhol 005 Forward CGCGGATCCCATATG-GACAATATTGACATGT BamHI- Ndel Reverse CCCGCTCGAG-CATCACATCCGCCCG Xhol 006 Forward CGCGGATCCCATATG-CTGCTGGTGCTGG BamHI- Ndel Reverse CCCGCTCGAG-AGTTCCGGCTTTGATGT Xhol 007 Forward CGCGGATCCCATATG-GCCGACAACAGCATCAT BamHI- Ndel Reverse CCCGCTCGAG-AAGGCGTTCATGATATAAG Xhol 008 Forward CGCGGATCCCATATG-AACAACAGACATTTTG BamHI- Ndel Reverse CCCGCTCGAG-CCTGTCCGGTAAAAGAC Xhol 009 Forward CGCGGATCCCATATG-CCCCGCGCTGCT BamHI- Ndel Reverse CCCGCTCGAG-TGGCTTTTGCCACGTTTT Xhol Oi l Forward CGCGGATCCCATATG-AAGACACACCGCAAG BamHI- Ndel Reverse CCCGCTCGAG-GGCGGTCAGTACGGT Xhol 012 Forward CGCGGATCCCATATG-CTCGCCCGTTGCC BamHI- Ndel Reverse CCCGCTCGAG-AGCGGGGAAGAGGCAC Xhol 013 Forward CGCGGATCCCATATG-CCTTTGACCATGCT BamHI- Ndel Reverse CCCGCTCGAG-CTGATTCGGCAAAAAAATCT Xhol 018 Forward CGCGGATCCCATATG-CAGCAGAGGCAGTT BamHI- Ndel Reverse CCCGCTCGAG-GACGAGGCGAACGCC Xhol 019 Forward AAAGAATTC-CTGCCAGCCGGCAAGACCCCGGC Eco RI Reverse AAACTGCAG-TCAGCGGGCGGGGACAATGCCCAT Pst l 023 Forward AAAGAATTC-AAAGAATATTCGGCATGGCAGGC Eco RI Reverse AAACTGCAG-TTACCCCCAAATCACTTTAACTGA Pst l 025 Forward AAAGAATTC-TGCGCCACCCAACAGCCTGCTCC Eco RI Reverse AAACTGCAG-TCAGAACGCGATATAGCTGTTCGG Pst l 031 Forward CGCGGATCCCATATG-GTCTCCCTTCGCTT BamHI- Ndel Reverse CCCGCTCGAG-ATGTAAGACGGGGACAAC Xhol 032 Forward CGCGGATCCCATATG-CGGCGAAACGTGC BamHI- Ndel Reverse CCCGCTCGAG-CTGGTTTTTTGATATTTGTG Xhol Forward CGCGGATCCCATATG-GCGGCGGCAGACA BamHI- Ndel Reverse CCCGCTCGAG-ATTTGCCGCATCCCGAT Xhol Forward CGCGGATCCCATATG-GCCGAAAACAGCTACGG BamHI- Ndel Reverse CCCGCTCGAG-TTTGACGATTTGGTTCAATT Xhol Forward CGCGGATCCCATATG-CTGAAGCCGTGCG BamHI- Ndel Reverse CCCGCTCGAG-CCGGACTGCGTATCGG Xhol Forward CGCGGATCCCATATG-ACCGATTTCCGCCA BamHI- Ndel Reverse CCCGCTCGAG-TTCTACGCCGTACTGCC Xhol Forward CGCGGATCCCATATG-CCGTCCGAACCGC BamHI- Ndel Reverse CCCGCTCGAG-TAGGATGACGAGGTAGG Xhol Forward CGCGGATCCCATATG-TTCGTGCGCGAACCGC BamHI- Ndel Reverse CCCGCTCGAG-GCCCAAAAACTCTTTCAAA Xhol Forward CGCGGATCCCATATG-ACGATGATTTGCTTGC BamHI- Ndel Reverse CCCGCTCGAG-TTTGCAGCCTGCATTTGAC Xhol Forward AAAAAAGGTACC-ATGGTTGTTTCAAATCAAAATATC Kpn I Reverse AAACTGCAG-TTATTGCGCTTCACCTTCCGCCGC Pst l a Forward AAAAAAGGTACC-GCAAAAGTGCATGGCGGCTTGGACGGTC iC Kpn I Reverse AAAAAACTGCAG- Pst l TTAATCCTGCAACACGAATTCGCCCGTCCG Forward CGCGGATCCCATATG-CCGTCCGACTAGAG BamHI- Ndel Reverse CCCGCTCGAG-ATGCGCTACGGTAGCCA Xhol Forward AAAGAATTC-ATGTCGGCAATGCTCCCGACAAG Eco RI Reverse AAACTGCAG-TCACTCGGCGACCCACACCGTGAA Pst l Forward CGCGGATCCCATATG-GTCATCATACAGGCG BamHI- Ndel Reverse CCCGCTCGAG-TCCGAAAAAGCCCATTTTG Xhol Forward AAAGAATTC-ATGCTCAACAAAGGCGAAGAATTGCC Eco RI Reverse AAACTGCAG-TCAAGATTCGACGGGGATGATGCC Pst l Forward AAAGAATTC-ATGCGGGCGCAGGCGTTTGATCAGCC Eco RI Reverse AAACTGCAG-AAGGCGTATCTGAAAAAATGGCAG Pst l Forward CGCGGATCCCATATG-GGCGCGGGCTGG BamHI- Ndel Reverse CCCGCTCGAG-AATCGGGCCATCTTCGA Xhol Forward AAAAAAGAATTC-ATGGCTTTGGTGGCGGAGGAAAC Eco RI Reverse AAAAAAGTCGAC-TCAGGCGGCGTTTTTCACCTTCCT Sai l a Forward AAAAAAGAATTC-GTGGCGGAGGAAACGGAAATATCCGC Eco RI Reverse AAAAAACTGCAG-TTAGCTGTTTTTGGAAACGCCGTCCAACCC Pst I 073 Forward CGCGGATCCCATATG-TGTATGCCATATAAGAT BamHI- Ndel Reverse CCCGCTCGAG-CACCGGATTGTCCGAC Xhol 075 Forward CGCGGATCCCATATG-CCGTCTTACTTCATC BamHI- Ndel Reverse CCCGCTCGAG-ATCACCAATGCCGATTATTT Xhol 077a Forward AAAAAAGAATTC-GGCGGCATTTTCATCGACACCTTCCT Eco RI Reverse AAAAAACTGCAG-TCAGACGAACATCTGCACAAACGCAAT Pst l 080 Forward AAAGAATTC-GCGTCCGGGCTGGTTTGGTTTTACAATTC Eco RI Reverse AAACTGCAG-CTATTCTTCGGATTCTTTTTCGGG Pst l 081 Forward AAAGAATTC-ATGAAACCACTGGACCTAAATTTCATCTG Eco RI Reverse AAACTGCAG-TCACTTATCCTCCAATGCCTC Pst l 082 Forward AAAGAATTC-ATGTGGTTGTTGAAGTTGCCTGC Eco RI Reverse AAACTGCAG-TTACGCGGATTCGGCAGTTGG Pst l 084 Forward AAAGAATTC-TATCACCCAGAATATGAATACGGCTACCG Eco RI Reverse AAACTGCAG-TTATACTTGGGCGCAACATGA Pst l 085 Forward CGCGGATCCCATATG-GGTAAAGGGCAGGACT BamHI- Ndel Reverse CCCGCTCGAG-CAAAGCCTTAAACGCTTCG Xhol 086 Forward AAAAAAGGTACC-TATTTGGCATCAAAAGAAGGCGG Kpn I Reverse AAACTGCAG-TTACTCCACCCGATAACCGCG Pst l 087 Forward AAAGAATTC-ATGGGCGGTAAAACCTTTATGC Eco RI Reverse AAACTGCAG-TTACGCCGCACACGCAATCGC Pst l 087a Forward AAAAAAGAATTC-AAGCTATTAGGCGTGCCGATTGTGATTCA Eco RI Reverse AAAAAACTGCAG-TTACGCCTGCAAGATGCCCAGCTTGCC Pst l 088 Forward AAAAAAGAATTC-ATGTTTTTATGGCTCGCACATTTCAG Eco RI Reverse AAAAAACTGCAG-TCAGCGGATTTTGAGGGTACTCAAACC Pst l 089 Forward CGCGGATCCCATATG-CCGCCCAAAATCAC BamHI- Ndel Reverse CCCGCTCGAG-TGCGCATACCAAAGCCA Xhol 090 Forward CGCGGATCCCATATG-CGCATAGTCGAGCA BamHI- Ndel Reverse CCCGCTCGAG-AGCAAAACGGCGGTACG Xhol 091 Forward AAAGAATTC-ATGGAAATACCCGTACCGCCGAGTCC Eco RI Reverse AAACTGCAG-TCAGCGCAGGGGGTAGCCCAAGCC Pst l 092 Forward AAAGAATTC-ATGTTTTTTATTTCAATCCG Eco RI Reverse AAACTGCAG-TCAAATCTGTTTCGACAATGC Pst l 093 Forward AAAGAATTC-ATGCAGAATTTTGGCAAAGTGGC Eco RI Reverse AAACTGCAG-CTATGGCTCGTCATACCGGGC Pst l 094 Forward AAAGAATTC-ATGCCGTCACGGAAGCGCATCAACTC Eco RI Reverse AAACTGCAG-TTATCCCGGCCATACCGCCGAACA Pst l 095 Forward AAAGAATTC-ATGTCCTTTCATTTGAACATGGACGG Eco RI Reverse AAACTGCAG-TCAACGCCGCAGGCACTAACGCCC Pst l 096 Forward AAAGAATTC-ATGGCTCGTCATACCGGGCAGGG Eco RI Reverse AAACTGCAG-TCAAAGGAAAAGGCCGTCTGAAAAGCG Pst l 097 Forward AAAGAATTC-ATGGACACTTCAAAACAAACACTGTTG Eco RI Reverse AAACTGCAG-TCAGCCCAAATACCAGAATTTCAG Pst l 098 Forward AAAGAATTC-GATGAACGCAGCCCAGCATGGATACG Eco RI Reverse AAACTGCAG-TTACGACATTCTGATTTGGCA Pst l 102 Forward AAAAAAGAATTC-GGCCTGATGATTTTGGAAGTCAACAC Eco RI Reverse AAAAAACTGCAG-TTATCCTTTAAATACGGGGACGAGTTC Pst l 105 Forward CGCGGATCCCATATG-TCCGCAAACGAATACG BamHI- Ndel Reverse CCCGCTCGAG-GTGTTCTGCCAGTTTCAG Xhol 107 Forward AAAAAAGAATTC- Eco RI CTGATGATTTTGGAAGTCAACACCCATTATCC Reverse AAAAAACTGCAG-TTATCCTTTAAATACGGGGACGAGTTC Pst l 107b Forward AAAAAAGAATTC- Eco RI GATACCCAAGCCCCCGCCGGCACAAACTACTG Reverse AAAAAACTGCAG- Pst l TTACGCGTCGCCTTTAAAGTATTTGAGCAGGCTGGAGAC 108 Forward AAAGAATTC-ATGTTGCCGGGCTTCAACCG Eco RI Reverse AAACTGCAG-TTAGCGGTACAGGTGTTTGAAGCA Pst l 108a Forward AAAAAAGAATTC-GGTAACACATTCGGCAGCTTAGACGGTGG Eco RI Reverse AAACTGCAG-TTAGCGGTACAGGTGTTTGAAGCA Pst l 109 Forward AAAGAATTC-ATGTATTATCGCCGGGTTATGGG Eco RI Reverse AAACTGCAG-CTAGCCCAAAGATTTGAAGTGTTC Pst l 111 Forward CGCGGATCCCATATG-TGTTCGGAACAAACCGC BamHI- Ndel Reverse CCCGCTCGAG-GCGGAGCAGTTTTTCAAA Xhol 114 Forward CGCGGATCCCATATG-GCTTCCATCACTTCGC BamHI- Ndel Reverse CCCGCTCGAG-CATCCGCGAAATCGTC Xhol 117 Forward AAAAAAGGTACC-ATGGTCGAAGAACTGGAACTGCTG Kpn I Reverse AAACTGCAG-TTAAAGCCGGGTAACGCTCAATAC Pst l 118 Forward AAAGTCGACATGTGTGAGTTCAAGGATATTATAAG Sai l Reverse AAAGCATGC-CTATTTTTTGTTGTAATAATCAAATC Sph l 121 Forward CGCGGATCCCATATG-GAAACACAGCTTTACAT BamHI- Ndel Reverse CCCGCTCGAG-ATAATAATATCCCGCGCCC Xhol 122 Forward CGCGGATCCCATATG-GTCATGATTAAAATCCGCA BamHI- Ndel Reverse CCCGCTCGAG-AATCTTGGTAGATTGGATTT Xhol 125 Forward AAAGAATTC-ATGTCGGGCAATGCCTCCTCTCC Eco RI Reverse AAACTGCAG-TCACGCCGTTTCAAGACG Pst l 125a Forward AAAAAAGAATTC- ACGGCAGGCAGCACCGCCGCACAGGTTTC Eco RI Reverse AAAAAACTGCAG- Pst l TTATTTTGCCACGTCGGTTTCTCCGGTGAACAACGC 126 Forward CGCGGATCCCATATG-CCGTCTGAAACCC BamHI- Ndel Reverse CCCGCTCGAG-ATATTCCGCCGAATGCC Xhol 127 Forward AAAGAATTC-ATGGAAATATGGAATATGTTGGACACTTG Eco RI Reverse AAACTGCAG-TTAAAGTGTTTCGGAGCCGGC Pst l 127a Forward AAAAAAGAATTC- AAGGAACTGATTATGTGTCTGTCGGG Eco RI Reverse AAACTGCAG-TTAAAGTGTTTCGGAGCCGGC Pst l 128 Forward CGCGGATCCCATATG-ACTGACAACGCACT BamHI- Ndel Reverse CCCGCTCGAG-GACCGCGTTGTCGAAA Xhol 130 Forward CGCGGATCCCATATG-AAACAACTCCGCGA BamHI- Ndel Reverse CCCGCTCGAG-GAATTTTGCACCGGATTG Xhol 132 Forward AAAGAATTC-ATGGAACCCTTCAAAACCTTAATTTG Eco RI Reverse AAAAAACTGCAG-TCACCATGTCGGCATTTGAAAAAC Pst l 134 Forward CGCGGATCCCATATG-TCCCAAGAAATCCTC BamHI- Ndel Reverse CCCGCTCGAG-CAGTTTGACCGAATGTTC Xhol 135 Forward CGCGGATCCCATATG-AAATACAAAAGAATCGTATT BamHI- Ndel Reverse CCCGCTCGAG-AAATTCGGTCAGAAGCAGG Xhol 137 Forward AAAAAAGGTACC-ATGATTACCCATCCCCAATTCGATCC Kpn I Reverse AAAAAACTGCAG-TCAGTGCTGTTTTTTCATGCCGAA Pst l 137a Forward AAAAAAGAATTC-GGCCGCAAACACGGCATCGGCTTCCT Eco RI Reverse AAAAAACTGCAG-TTAAGCGGGATGACGCGGCAGCATACC Pst l 138 Forward AAAAAAGAATTC-AACTCAGGCGAAGGAGTGCTTGTGGC Eco RI Reverse AAAAAATCTAGA-TCAGTTTAGGGATAGCAGGCGTAC Xba l 141 Forward AAAGAATTC-ATGAGCTTCAAAACCGATGCCGAAATCGC Eco RI Reverse AAACTGCAG-TCAGAACAAGCCGTGAATCACGCC Pst l 142 Forward CGCGGATCCCATATG-CGTGCCGATTTCATG BamHI- Ndel Reverse CCCGCTCGAG-AAACTGCTGCACATGGG Xhol 143 Forward AAAAAAGAATTC- Eco RI ATGCTCAGTTTCGGCTTTCTCGGCGTTCAGAC Reverse AAAAAACTGCAG-TCAAACCCCGCCGTGTGTTTCTTTAAT Pst l 144 Forward AAAAAAGAATTC-GGTCTGATCGACGGGCGTGCCGTAAC Eco RI Reverse AAAAAATCTAGA-TCGGCATCGGCCGGCATATGTCCG Xba l 146 Forward AAAAAAGAATTC- Eco RI CGCCAAGTCGTCATTGACCACGACAAAGTC Reverse AAAAAACTGCAG-TTAGGCATCGGCAAATAGGAAACTGGG Pst l 147 Forward AAAAAAGAATTC-ACTGAGCAATCGGTGGATTTGGAAAC Eco RI Reverse AAAAAATCTAGA-TTAGGTAAAGCTGCGGCCCATTTGCGG Xba l 148 Forward AAAAAAGAATTC- Eco RI ATGGCGTTAAAAACATCAAACTTGGAACACGC Reverse AAAAAATCTAGA-TCAGCCCTTCATACAGCCTTCGTTTTG Xba l 149 Forward CGCGGATCCCATATG-CTGCTTGACAACAAAGT BamHI- Ndel Reverse CCCGCTCGAG-AAACTTCACGTTCACGCC Xhol 150 Forward CGCGGATCCCATATG-CAGAACACAAATCCG BamHI- Ndel Reverse CCCGCTCGAG-ATAAACATCACGCTGATAGC Xhol 151 Forward AAAAAAGAATTC- Eco RI ATGAAACAAATCCGCAACATCGCCATCATCGC Reverse AAAAAACTGCAG-TCAATCCAGCTTTTTAAAGTGGCGGCG Pst l 152 Forward AAAAAAGAATTC- Eco RI ATGAAAAACAAAACCAAAGTCTGGGACCTCCC Reverse AAAAAACTGCAG-TCAGGACAGGAGCAGGATGGCGGC Pst l 153 Forward AAAAAAGAATTC-ATGGCGTTTGCTTACGGTATGAC Eco RI Reverse AAAAAACTGCAG-TCAGTCATGTTTTTCCGTTTCATT Pst l 153a Forward AAAAAAGAATTC-CGGACTTCGGTATCGGTTCCCCAGCATTG Eco RI Reverse AAAAAACTGCAG- Pst l TTACGCCGACGAAATACTCAGACTTTTCGG 154 Forward CGCGGATCCCATATG-ACTGACAACAGCCC BamHI- Ndel Reverse CCCGCTCGAG-TCGGCTTCCTTTCGGG Xhol 155 Forward AAAAAAGAATTC-ATGAAAATCGGTATCCCACGCGAGTC Eco RI Reverse AAAAAACTGCAG-TTACCCTTTCTTAAACATATTCAGCAT Pst l 156 Forward AAAAAAGAATTC-GCACAGCAAAACGGTTTTGAAGC Eco RI Reverse AAAAAACTGCAG-TCAAGCAGCCGCGACAAACAGCCC Pst l 157 Forward CGCGGATCCCATATG-AGGAACGAGGAAAAAC BamHI- Ndel Reverse CCCGCTCGAG-AAAACACAATATCCCCGC Xhol 158 Forward AAAAAAGAATTC-GCGGAGCAGTTGGCGATGGCAAATTCTGC Eco RI Reverse AAAAAATCTAGA-TTATCCACAGAGATTGTTTCCCAGTTC Xba l 160 Forward CGCGGATCCCATATG-GACATTCTGGACAAAC BamHI- Ndel Reverse CCCGCTCGAG-TTTTTGCCCGCCTTCTTT Xhol 163 Forward AAAAAAGGTACC-ACCGTGCCGGATCAGGTGCAGATGTG Kpn I Reverse AAAAAATCTAGA-TTACTCTGCCAATTCCACCTGCTCGTG Xba l 163a Forward AAAAAAGAATTC-CGGCTGGTGCAGATAATGAGCCAGAC Eco RI Reverse AAAAAATCTAGA-TTACTCTGCCAATTCCACCTGCTCGTG Xba l 164 Forward CGCGGATCCCATATG-AACCGGACTTATGCC BamHI- Ndel Reverse CCCGCTCGAG-TTTGTTTCCGTCAAACTGC Xhol 165 Forward CGCGGATCCGCTAGC-GCTGAAGCGACAGACG BamHI- Nhel Reverse CCCGCTCGAG-AATATCCAATACTTTCGCG Xhol 206 Forward CGCGGATCCCATATG-AAACACCGCCAACCGA BamHI- Ndel Reverse CCCGCTCGAG-TTCTGTAAAAAAAGTATGTGC Xhol 209 Forward CGCGGATCCCATATG-CTGCGGCATTTAGGA BamHI- Ndel Reverse CCCGCTCGAG-TACCCCTGAAGGCAAC Xhol 211 Forward AAAAAAGAATTC-ATGTTGCGGGTTGCTGCTGC Eco RI Reverse AAAAAACTGCAG-CTATCCTGCGGATTGGCATTGAAA Pst l 212 Forward CGCGGATCCCATATG-GACAATCTCGTATGG BamHI- Ndel Reverse CCCGCTCGAG-AGGGGTTAGATCCTTCC Xhol 215 Forward CGCGGATCCCATATG-GCATGGTTGGGTCGT BamHI- Ndel Reverse CCCGCTCGAG-CATATCTTTTGTATCATAAATC Xhol 216 Forward CGCGGATCCCATATG-GCAATGGCAGAAAACG BamHl- Ndel Reverse CCCGCTCGAG-TACAATCCGTGCCGCC Xhol 217 Forward CGCGGATCCCATATG-GCGGATGACGGTGTG BamHI- Ndel Reverse CCCGCTCGAG-ACCCCGAATATCGAATCC Xhol 218 Forward CGCGGATCCCATATG-GTCGCGGTCGATC BamHI- Ndel Reverse CCCGCTCGAG-TAACTCATAGAATCCTGC Xhol 219 Forward CGCGGATCCGCTAGC-ACGGCAAGGTTAAG BamHI- Nhel Reverse CCCGCTCGAG-TTTAAACCATCTCCTCAAAAC Xhol 223 Forward CGCGGATCCCATATG-GAATTCAGGCACCAAGTA BamHI- Ndel Reverse CCCGCTCGAG-GGCTTCCCGCGTGTC Xhol 225 Forward CGCGGATCCCATATG-GACGAGTTGACCAACC BamHI- Ndel Reverse CCCGCTCGAG-GTTCAGAAAGCGGGAC Xhol 226 Forward AAAGAATTC-CTTGCGATTATCGTGCGCACGCG Eco RI Reverse AAACTGCAG-TCAAAATCCCAAAACGGGGAT Pst l 228 Forward CGCGGATCCCATATG-TCGCAAGAAGCCAAACAG BamHI- Ndel Reverse CCCGCTCGAG-TTTGGCGGCATCTTTCAT Xhol 229 Forward CGCGGATCCCATATG-CAAGAGGTTTTGCCC BamHI- Ndel Reverse CCCGCTCGAG-ACACAATATAGCGGATGAAC Xhol 230 Forward CGCGGATCCCATATG-CATCCGGGTGCCGAC BamHI- Ndel Reverse CCCGCTCGAG-AAGTTTGGCGGCTTCGG Xhol 232 Forward AAAAAAGAATTC-ATGTACGCTAAAAAAGGCGGTTTGGG Eco RI Reverse AAAAAACTGCAG-TCAAGGTTTTTTCCTGATTGCCGCCGC Pst l 232a Forward AAAAAAGAATTC-GCCAAGGCTGCCGATACACAAATTGA Eco RI Reverse AAAAAACTGCAG-TTAAACATTGTCGTTGCCGCCCAGATG Pst l 233 Forward CGCGGATCCCATATG-GCGGACAAACCCAAG BamHI- Ndel Reverse CCCGCTCGAG-GACGGCATTGAGCAG Xhol 234 Forward CGCGGATCCCATATG-GCCGTTTCACTGACCG BamHI- Ndel Reverse GCCCAAGCTT-ACGGTTGGATTGCCATG Hind III 235 Forward CGCGGATCCCATATG-GCCTGCCAAGTTCAAA BamHI- Ndel Reverse CCCGCTCGAG-TTTGGGCTGCTCTTC Xhol 236 Forward CGCGGATCCCATATG-GCGCGTTTCGCCTT BamHI- Ndel Reverse CCCGCTCGAG-ATGGGTCGCGCGCCGT Xhol 238 Forward CGCGGATCCGCTAGC-AACGGTTTGGATGCCCG BamHI- Nhel Reverse CCCGCTCGAG-TTTGTCTAAGTTCCTGATATG Xhol 239 Forward CCGGAATTCTACATATG-CTCCACCATAAAGGTATTG EcoRI- Ndel Reverse CCCGCTCGAG-TGGTGAAGAGCGGTTTAG Xhol 240 Forward CGCGGATCCCATATG-GACGTTGGACGATTTC BamHI- Ndel Reverse CCCGCTCGAG-AAACGCCATTACCCGATG Xhol 241 Forward CCGGAATTCTACATATG-CCAACACGTCCAACT EcoRI- Ndel Reverse CCCGCTCGAG-GAATGCGCCTGTAATTAATC Xhol 242 Forward CGCGGATCCCATATG-ATCGGCAAACTTGTTG BamHI- Ndel Reverse GCCCAAGCTT-ACCGATACGGTCGCAG Hindlll 243 Forward CGCGGATCCCATATG-ACGATTTTTTCGATGCTGC BamHI- Ndel Reverse CCCGCTCGAG-CGACTTGGTTACCGCG Xhol 244 Forward CGCGGATCCCATATG-CCGTCTGAAGCCC BamHI- Ndel Reverse CCCGCTCGAG-TTTTTTCGGTAGGGGATTT Xhol 246 Forward CGCGGATCCCATATG-GACATCGGCAGTGC BamHI- Ndel Reverse CCCGCTCGAG-CCCGCGCTGCTGGAG Xhol 247 Forward CGCGGATCCCATATG-GTCGGATCGAGTTAC BamHI- Ndel Reverse CCCGCTCGAG-AAGTGTTCTGTTTGCGCA Xhol 248 Forward CGCGGATCCCATATG-CGCAAACAGAACACT BamHI- Ndel Reverse CCCGCTCGAG-CTCATCATTATTGCTAACA Xhol 249 Forward CGCGGATCCCATATG-AAGAATAATGATTGCTTC BamHI- Ndel Reverse CCCGCTCGAG-TTCCCGACCTCCGAC Xhol 251 Forward CGCGGATCCCATATG-CGTGCTGCGGTAGT BamHI- Ndel Reverse CCCGCTCGAG-TACGAAAGCCGGTCGTG Xhol 253 Forward AAAAAAGAATTC-ATGATTGACAGGAACCGTATGCTGCG Eco RI Reverse AAAAAACTGCAG-TTATTGGTCTTTCAAACGCCCTTCCTG Pst l 253a Forward AAAAAAGAATTC-AAAATCCTTTTGAAAACAAGCGAAAACGC } Eco RI Reverse AAAAAACTGCAG-TTATTGGTCTTTCAAACGCCCTTCCTG Pst l 254 Forward AAAAAAGAATTC-ATGTATACAGGCGAACGCTTCAATAC Eco RI Reverse AAAAAATCTAGA-TCAGATTACGTAACCGTACACGCTGAC Xba l 255 Forward CGCGGATCCCATATG-GCCGCGTTGCGTTAC BamHI- Ndel Reverse CCCGCTCGAG-ATCCGCAATACCGACCAG Xhol 256 Forward CGCGGATCCGCTAGC-TTTTAACACCGCCGGAC BamHI- Nhel Reverse CCCGCTCGAG-ACGCCTGTTTGTGCGG Xhol 257 Forward CGCGGATCCCATATG-GCGGTTTCTTTCCTG BamHI- Ndel Reverse CCCGCTCGAG-GCGCGTGAATATCGCG Xhol 258 Forward AAAAAAGAATTC-GATTATTTCTGGTGGATTGTTGCGTTCAG Eco RI Reverse AAAAAACTGCAG-CTACGCATAAGTTTTTACCGTTTTTGG Pst l 258a Forward AAAAAAGAATTC-GCGAAGGCGGTGGCGCAAGGCGA Eco RI Reverse AAAAAACTGCAG-CTACGCATAAGTTTTTACCGTTTTTGG Pst l 259 Forward CGCGGATCCCATATG-GAAGAGCTGCCTCCG BamHI- Ndel Reverse CCCGCTCGAG-GGCTTTTCCGGCGTTT Xhol 260 Forward CGCGGATCCCATATG-GGTGCGGGTATGGT BamHI- Ndel Reverse CCCGCTCGAG-AACAGGGCGACACCCT Xhol 261 Forward AAAAAAGAATTC-CAAGATACAGCTCGGGCATTCGC Eco RI Reverse AAAAAACTGCAG-TCAAACCAACAAGCCTTGGTCACT Pst l 263 Forward CGCGGATCCCATATG-GCACGTTTAACCGTA BamHI- Ndel Reverse CCCGCTCGAG-GGCGTAAGCCTGCAATT Xhol 264 Forward AAAAAAGGTACC-GCCGACGCAGTGGTCAAGGCAGAA Kpn I Reverse AAACTGCAG-TCAGCCGGCGGTCAATACCGCCCG Pst l 265 Forward AAAAAAGAATTC-GCGGAGGTCAAGAGAAGGTGTTTG Eco RI Reverse AAAAAACTGCAG-TTACGAATACGTCGTCAAAATGGG Pst l 266 Forward AAAGAATTC-CTCATCTTTGCCAACGCCCCCTTC Eco RI Reverse AAACTGCAG-CTATTCCCTGTTGCGCGTGTGCCA Pst l 267 Forward AAAGAATTC-TTCTTCCGATTCGATGTTAATCG Eco RI Reverse AAACTGCAG-TTAGTAAAAACCTTTCTGCTTGGC Pst l 269 Forward AAAGAATTC-TGCAAACCTTGCGCCACGTGCCC Eco RI Reverse AAACTGCAG-TTACGAAGACCGCAACGAAAGGCAGAG Pst l 269a Forward AAAAAAGAATTC-GACTTTATCCAAAACACGGCTTCGCC Eco RI Reverse AAACTGCAG-TTACGAAGACCGCAACGAAAGGCAGAG Pst l 270 Forward AAAGAATTC-GCCGTCAAGCTCGTTTTGTTGCAATG Eco RI Reverse AAACTGCAG-TTATTCGGCGGTAAATGCCGTCTG Pst l 271 Forward CGCGGATCCCATATG-CCTGTGTGCAGCTCGAC BamHI- Ndel Reverse CCCGCTCGAG-TCCCAGCCCCGTGGAG Xhol 272 Forward AAAGAATTC-ATGACCGCAAAGGAAGAACTGTTCGC Eco RI Reverse AAACTGCAG-TCAGAGCAGTTCCAAATCGGGGCT Pst l 273 Forward AAAGAATTC-ATGAGTCTTCAGGCGGTATTTATATACCC Eco RI Reverse AAACTGCAG-TTACGCGTAAGAAAAAACTGC Pst l 274 Forward CGCGGATCCCATATG-ACAGATTTGGTTACGGAC BamHI- Ndel Reverse CCCGCTCGAG-TTTGCTTTCAGTATTATTGAA Xhol 276 Forward AAAAAAGAATTC- Eco RI ATGATTTTGCCGTCGTCCATCACGATGATGCG Reverse AAAAAACTGCAG-CTACACCACCATCGGCGAATTTATGGC Pst l 277 Forward AAAAAAGAATTC-ATGCCCCGCTTTGAGGACAAGCTCGTAGG Eco RI Reverse AAAAAACTGCAG-TCATAAGCCATGCTTACCTTCCAACAA Pst l 277a Forward AAAAAAGAATTC-GGGGCGGCGGCTGGGTTGGACGTAGG Eco RI Reverse AAAAAACTGCAG-TCATAAGCCATGCTTACCTTCCAACAA Pst l 278 Forward AAAAAAGGTACC-GTCAAAGTTGTATTAATCGGGCCTTTGCC Kpn I Reverse AAAAAACTGCAG-TCATTCAACCATATCAAATCTGCC Pst l 278a Forward AAAAAAGAATTC-AAAACTCTCCTAATTCGTCATAGTCG Eco RI Reverse AAAAAACTGCAG-TCATTCAACCATATCAAATCTGCC Pst l 279 Forward CGCGGATCCCATATG-TTGCCTGCAATCACGATT BamHI- Ndel Reverse CCCGCTCGAG-TTTAGAAGCGGGCGGCAA Xhol 280 Forward AAAAAAGGTACC-GCCCCCCTGCCGGTTGTAACCAG Kpn I Reverse AAAAAACTGCAG-TTATTGCTTCATCGCGTTGGTCAAGGC Pst l 281 Forward AAAAAAGAATTC-GCACCCGTCGGCGTATTCCTCGTCATGCG Eco RI Reverse AAAAAATCTAGA-GGTCAGAATGCCGCCTTCTTTGCCGAG Xba l 281a Forward AAAAAAGAATTC-TCCTACCACATCGAAATTCCTTCCGG Eco RI Reverse AAAAAATCTAGA-GGTCAGAATGCCGCCTTCTTTGCCGAG Xba l 282 Forward AAAAAAGAATTC-CTTTACCTTGACCTGACCAACGGGCACAG Eco RI Reverse AAAAAACTGCAG-TCAACCTGCCAGTTGCGGGAATATCGT Pst l 283 Forward CGCGGATCCCATATG-GCCGTCTTTACTTGGAAG BamHI- Ndel Reverse CCCGCTCGAG-ACGGCAGTATTTGTTTACG Xhol 284 Forward CGCGGATCCCATATG-TTTGCCTGCAAAAGAATCG BamHI- Ndel Reverse CCCGCTCGAG-CCGACTTTGCAAAAACTG Xhol 286 Forward CGCGGATCCCATATG-GCCGACCTTTCCGAAAA BamHI- Ndel Reverse CCCGCTCGAG-GAAGCGCGTTCCCAAG Xhol 287 Forward CCGGAATTCTAGCTAGC-CTTTCAGCCTGCGGG EcoRI- Nhel Reverse CCCGCTCGAG-ATCCTGCTCTTTTTTGCC Xhol 288 Forward CGCGGATCCCATATG-CACACCGGACAGG BamHI- Ndel Reverse CCCGCTCGAG-CGTATCAAAGACTTGCGT Xhol 290 Forward CGCGGATCCCATATG-GCGGTTTGGGGCGGA BamHI- Ndel Reverse CCCGCTCGAG-TCGGCGCGGCGGGC Xhol 292 Forward CGCGGATCCCATATG-TGCGGGCAAACGCCC BamHI- Ndel Reverse CCCGCTCGAG-TTGATTTTTGCGGATGATTT Xhol 294 Forward AAAAAAGAATTC-GTCTGGTCGATTCGGGTTGTCAGAAC Eco RI Reverse AAAAAACTGCAG-TTACCAGCTGATATAAAACATCGCTTT Pst l 295 Forward CGCGGATCCCATATG-AACCGGCCGGCCTCC BamHI- Ndel Reverse CCCGCTCGAG-CGATATTTGATTCCGTTGC Xhol 297 Forward AAAAAAGAATTC-GCATACATTGCTTCGACAGAGAG Eco RI Reverse AAAAAACTGCAG-TCAATCCGATTGCGACACGGT Pst l 298 Forward AAAAAAGAATTC-CTGATTGCCGTGTGGTTCAGCCAAAACCC Eco RI Reverse AAAAAACTGCAG-TCATGGCTGTGTACTTGATGGTTGCGT Pst l 299 Forward CGCGGATCCGCTAGC-CTACCTGTCGCCTCCG BamHI- Nhel Reverse CCCGCTCGAG-TTGCCTGATTGCAGCGG Xhol 302 Forward AAAAAAGAATTC-ATGAGTCAAACCGATACGCAACG Eco RI Reverse AAAAAACTGCAG-TTAAGGTGCGGGATAGAATGTGGGCGC Pst l 305 Forward AAAAAAGGTACC-GAATTTTTACCGATTTCCAGCACCGGA Kpn I Reverse AAAAAACTGCAG-TCATTCCCAACTTATCCAGCCTGACAG Pst l 305a Forward AAAAAAGGTACC-TCCCGTTCGGGCAGTACGATTATGGG Kpn I Reverse AAAAAACTGCAG-TTACAAACCGACATCATGCAGGGTGAA Pst l 306 Forward CGCGGATCCCATATG-TTTATGAACAAATTTTCCC BamHΪ- Ndel Reverse CCCGCTCGAG-CCGCATCGGCAGAC Xhol 308 Forward CGCGGATCCCATATG-TTAAATCGGGTATTTTATC BamHI- Ndel Reverse CCCGCTCGAG-ATCCGCCATTCCCTGC Xhol 311 Forward AAAAAAGGTACC-ATGTTCAGTTTTGGCTGGGTGTTT Kpn I Reverse AAACTGCAG-ATGTTCATATTCCCTGCCTTCGGC Pst l 312 Forward AAAAAAGGTACC-ATGAGTATCCCATCCGGCGAAATT Kpn I Reverse AAACTGCAG-TCAGTTTTTCATCGATTGAACCGG Pst l 313 Forward AAAAAAGAATTC-ATGGACGACCCGCGCACCTACGGATC Eco RI Reverse AAAAAACTGCAG-TCAGCGGCTGCCGCCGATTTTGCT Pst l 401 Forward CGCGGATCCCATATG-AAGGCGGCAACACAGC BamHI- Ndel Reverse CCCGCTCGAG-CCTTACGTTTTTCAAAGCC Xhol 402 Forward AAAAAAGAATTC-GTGCCTCAGGCATTTTCATTTACCCTTGC Eco RI Reverse AAAAAATCTAGA-TTAAATCCCTCTGCCGTATTTGTATTC Xba l 402a Forward AAAAAAGAATTC-AGGCTGATTGAAAACAAACACGG Eco RI Reverse AAAAAATCTAGA-TTAAATCCCTCTGCCGTATTTGTATTC Xba l 406 Forward CGCGGATCCCATATG-TGCGGGACACTGACAG BamHI- Ndel Reverse CCCGCTCGAG-AGGTTGTCCTTGTCTATG Xhol 501 Forward CGCGGATCCCATATG-GCAGGCGGAGATGGC BamHI- Ndel Reverse CCCGCTCGAG-GGTGTGATGTTCACCC Xhol 502 Forward CGCGGATCCCATATG-GTAGACGCGCTTAAGCA BamHI- Ndel Reverse CCCGCTCGAG-AGCTGCATGGCGGCG Xhol 503 Forward CGCGGATCCCATATG-TGTTCGGGGAAAGGCG BamHI- Ndel Reverse CCCGCTCGAG-CCGCGCATTCCTCGCA Xhol 504 Forward CGCGGATCCCATATG-AGCGATATTGAAGTGACG BamHI- Ndel Reverse GCCCAAGCTT-TGATTCAAGTCCTTGCCG Hindlll 505 Forward CGCGGATCCCATATG-TTTCGTTTACAATTCAGG BamHI- Ndel Reverse CCCGCTCGAG-CGGCGTTTTATAGCGG Xhol 510 Forward CGCGGATCCCATATG-CCTTCGCGGACAC BamHI- Ndel Reverse CCCGCTCGAG-GCGCACTGGCAGCG Xhol 512 Forward CGCGGATCCCATATG-GGACATGAAGTAACGGT BamHI- Ndel Reverse CCCGCTCGAG-AGGAATAGCCTTTGACG Xhol 515 Forward CGCGGATCCCATATG-GAGGAAATAGCCTTCGA BamHI- Ndel Reverse CCCGCTCGAG-AAATGCCGCAAAGCATC Xhol 516 Forward CGCGGATCCCATATG-TGTACGTTGATGTTGTGG BamHI- Ndel Reverse CCCGCTCGAG-TTTGCGGGCGGCATC Xhol 517 Forward CGCGGATCCCATATG-GGTAAAGGTGTGGAAATA BamHI- Ndel Reverse CCCGCTCGAG-GTGCGCCCAGCCGT Xhol 518 Forward AAAGAATTC-GCTTTTTTACTGCTCCGACCGGAAGG Eco RI Reverse AAACTGCAG-TCAAATTTCAGACTCTGCCAC Pst l 519 Forward CGCGGATCCCATATG-TTCAAATCCTTTGTCGTCA BamHI- Ndel Reverse CCCGCTCGAG-TTTGGCGGTTTTGCTGC Xhol 520 Forward CGCGGATCCCATATG-CCTGCGCTTCTTTCA BamHI- Ndel Reverse CCCGCTCGAG-ATATTTACATTTCAGTCGGC Xhol 521 Forward CGCGGATCCCATATG-GCCAAAATCTATACCTGC BamHI- Ndel Reverse CCCGCTCGAG-CATACGCCCCAGTTCC Xhol 522 Forward CGCGGATCCCATATG-ACTGAGCCGAAACAC BamHI- Ndel Reverse GCCCAAGCTT-TTCTGATTTCAAATCGGCA Hindlll 523 Forward CGCGGATCCCATATG-GCTCTGCTTTCCGCG BamHI- Ndel Reverse CCCGCTCGAG-AGGGTGTGTGATAATAAGAAG Xhol 525 Forward CGCGGATCCCATATG-GCCGAAATGGTTCAAATC BamHI- Ndel Reverse CCCGCTCGAG-GCCCGTGCATATCATAAA Xhol 527 Forward AAAGAATTC-TTCCCTCAATGTTGCCGTTTTCG Eco RI Reverse AAACTGCAG-TTATGCTAAACTCGAAACAAATTC Pst l 529 Forward CGCGGATCCGCTAGC-TGCTCCGGCAGCAAAAC BamHI- Nhel Reverse GCCCAAGCTT-ACGCAGTTCGGAATGGAG Hindlll 530 Forward CGCGGATCCCATATG-AGTGCGAGCGCGG BamHI- Ndel Reverse CCCGCTCGAG-ACGACCGACTGATTCCG Xhol 531 Forward AAAAAAGAATTC-TATGCCGCCGCCTACCAAATCTACGG Eco RI Reverse AAAAAACTGCAG-TTAAAACAGCGCCGTGCCGACGACAAG Pst l 532 Forward AAAAAAGAATTC-ATGAGCGGTCAGTTGGGCAAAGGTGC Eco RI Reverse AAAAAACTGCAG-TCAGTGTTCCAAGTGGTCGGTATCAAA Pst l 532a Forward AAAAAAGAATTC-TTGGGTGTCGCGTTTGAGCCGGAAGT Eco RI Reverse AAAAAACTGCAG-TCAGTGTTCCAAGTGGTCGGTATCAAA Pst l 535 Forward AAAGAATTC-ATGCCCTTTCCCGTTTTCAGAC Eco RI Reverse AAACTGCAG-TCAGACGACCCCGCCTTCCCC Pst l 537 Forward CGCGGATCCCATATG-CATACCCAAAACCAATCC BamHI- Ndel Reverse CCCGCTCGAG-ATCCTGCAAATAAAGGGTT Xhol 538 Forward CGCGGATCCCATATG-GTCGAGCTGGTCAAAGC BamHI- Ndel Reverse CCCGCTCGAG-TGGCATTTCGGTTTCGTC Xhol 539 Forward CGCGGATCCGCTAGC-GAGGATTTGCAGGAAA BamHI- Nhel Reverse CCCGCTCGAG-TACCAATGTCGGCAAATC Xhol 542 Forward AAAGAATTC-ATGCCGTCTGAAACCGTGTC Eco RI Reverse AAACTGCAG-TTACCGCGAACCGGTCAGGAT Pst l 543 Forward AAAAAAGAATTC-GCCTTCGATGGCGACGTTGTAGGTAC Eco RI Reverse AAAAAATCTAGA- Xba l TTAATGAAGAAGAACATATTGGAATTTTGG 543a Forward AAAAAAGAATTC-GGCAAAACTCGTCATGAATTTGC Eco RI Reverse AAAAAATCTAGA- Xba l TTAATGAAGAAGAACATATTGGAATTTTGG 544 Forward AAAGAATTC-GCGCCCGCCTTCTCCCTGCCCGACCTGCACGG Eco RI Reverse AAACTGCAG-CTATTGCGCCACGCGCGTATCGAT Pst l 544a Forward AAAAAAGAATTC- Eco RI GCAAATGACTATAAAAACAAAAACTTCCAAGTACTTGC Reverse AAACTGCAG-CTATTGCGCCACGCGCGTATCGAT Pst l 547 Forward AAAGAATTC-ATGTTCGTAGATAACGGATTTAATAAAAC Eco RI Reverse AAACTGCAG-TTAACAACAAAAAACAAACCGCTT Pst l 548 Forward AAAGAATTC-GCCTGCAAACCTCAAGACAACAGTGCGGC Eco RI Reverse AAACTGCAG-TCAGAGCAGGGTCCTTACATCGGC Pst l 550 Forward AAAAAAGTCGAC- Sai l ATGATAACGGACAGGTTTCATCTCTTTCATTTTCC Reverse AAACTGCAG-TTACGCAAACGCTGCAAAATCCCC Pst l 550a Forward AAAAAAGAATTC-GTAAATCACGCCTTTGGAGTCGCAAACGG Eco RI Reverse AAACTGCAG-TTACGCAAACGCTGCAAAATCCCC Pst l 552 Forward AAAAAAGAATTC-TTGGCGCGTTGGCTGGATAC Eco RI Reverse AAACTGCAG-TTATTTCTGATGCCTTTTCCCAAC Pst l 554 Forward CGCGGATCCCATATG-TCGCCCGCGCCCAAC BamHI- Ndel Reverse CCCGCTCGAG-CTGCCCTGTCAGACAC Xhol 556 Forward AAAGAATTC-GCGGGCGGTTTTGTTTGGACATCCCG Eco RI Reverse AAACTGCAG-TTAACGGTGCGGACGTTTCTGACC Pst l 557 Forward CGCGGATCCCATATG-TGCGGTTTCCACCTGAA BamHI- Ndel Reverse CCCGCTCGAG-TTCCGCCTTCAGAAAGG Xhol 558 Forward AAAGAATTC-GAGCTTTATATGTTTCAACAGGGGACGGC Eco RI Reverse AAACTGCAG-CTAAACAATGCCGTCTGAAAGTGGAGA Pst l 558a Forward AAAAAAGAATTC-ATTAGATTCTATCGCCATAAACAGACGGG Eco RI Reverse AAAAAACTGCAG-CTAAACAATGCCGTCTGAAAGTGGAGA Pst l 560 Forward AAAAAAGAATTC- Eco RI TCGCCTTTCCGGGACGGGGCGCACAAGATGGC Reverse AAAAAACTGCAG-TCATGCGGTTTCAGACGGCATTTTGGC Pst l 561 Forward CCGGAATTCTACATATG-ATACTGCCAGCCCGT EcoRI- Ndel Reverse CCCGCTCGAG-TTTCAAGCTTTCTTCAGATG Xhol 562 Forward CGCGGATCCCATATG-GCAAGCCCGTCGAG BamHl- Ndel Reverse CCCGCTCGAG-AGACCAACTCCAACTCGT Xhol 565 Forward CGCGGATCCCATATG-AAGTCGAGCGCGAAATAC BamHI- Ndel Reverse CCCGCTCGAG-GGCATTGATCGGCGGC Xhol 566 Forward CGCGGATCCCATATG-GTCGGTGGCGAAGAGG BamHI- Ndel Reverse CCCGCTCGAG-CGCATGGGCGAAGTCA Xhol 567 Forward CCGGAATTCTACATATG-AGTGCGAACATCCTTG EcoRI- Ndel Reverse CCCGCTCGAG-TTTCCCCGACACCCTCG Xhol 568 Forward CGCGGATCCCATATG-CTCAGGGTCAGACC BamHI- Ndel Reverse CCCGCTCGAG-CGGCGCGGCGTTCAG Xhol 569 Forward AAAAAAGAATTC-CTGATTGCCTTGTGGGAATATGCCCG Eco RI Reverse AAAAAACTGCAG-TTATGCATAGACGCTGATAACGGCAAT Pst l 570 Forward CGCGGATCCCATATG-GACACCTTCCAAAAAATCG BamHI- Ndel Reverse CCCGCTCGAG-GCGGGCGTTCATTTCTTT Xhol 571 Forward AAAAAAGAATTC- Eco RI ATGGGTATTGCCGGCGCCGTAAATGTTTTGAACCC Reverse AAAAAACTGCAG-TTATGGCCGACGCGCGGCTACCTGACG Pst l 572 Forward CGCGGATCCCATATG-GCGCAAAAAGGCAAAACC BamHI- Ndel Reverse CCCGCTCGAG-GCGCAGTGTGCCGATA Xhol 573 Forward CGCGGATCCCATATG-CCCTGTTTGTGCCG BamHI- Ndel Reverse CCCGCTCGAG-GACGGTGTCATTTCGCC Xhol 574 Forward CGCGGATCCCATATG-TGGTTTGCCGCCCGC BamHI- Ndel Reverse CCCGCTCGAG-AACTTCGATTTTATTCGGG Xhol 575 Forward CGCGGATCCCATATG-GTTTCGGGCGAGG BamHI- Ndel Reverse CCCGCTCGAG-CATTCCGAATCTGAACAG Xhol 576 Forward CGCGGATCCCATATG-GCCGCCCCCGCATCT BamHI- Ndel Reverse CCCGCTCGAG-ATTTACTTTTTTGATGTCGAC Xhol 577 Forward CGCGGATCCCATATG-GAAAGGAACGGTGTATTT BamHI- Ndel Reverse CCCGCTCGAG-AGGCTGTTTGGTAGATTCG Xhol 578 Forward CGCGGATCCCATATG-AGAAGGTTCGTACAG BamHI- Ndel Reverse CCCGCTCGAG-GCCAACGCCTCCACG Xhol 579 Forward CGCGGATCCCATATG-AGATTGGGCGTTTCCAC BamHI- Ndel Reverse CCCGCTCGAG-AGAATTGATGATGTGTATGT Xhol 580 Forward CGCGGATCCCATATG-AGGCAGACTTCGCCGA BamHI- Ndel Reverse CCCGCTCGAG-CACTTCCCCCGAAGTG Xhol 581 Forward CGCGGATCCCATATG-CACTTCGCCCAGC BamHI- Ndel Reverse CCCGCTCGAG-CGCCGTTTGGCTTTGG Xhol 582 Forward AAAAAAGAATTC-TTTGGAGAGACCGCGCTGCAATGCGC Eco RI Reverse AAAAAATCTAGA-TCAGATGCCGTCCCAGTCGTTGAA Xba l 583 Forward AAAAAAGAATTC-ACTGCCGGCAATCGACTGCATAATCG Eco RI Reverse AAAAAACTGCAG-TTAACGGAGGTCAATATGATGAAATTG Pst l 584 Forward AAAAAAGAATTC- Eco RI GCGGCTGAAGCATTGAATTACAATATTGTC Reverse AAAAAACTGCAG-TCAGAACTGAACCGTCCCATTGACGCT Pst l 585 Forward AAAAAAGGTACC-TCTTTCTGGCTGGTGCAGAACACCCTTGC Eco RI Reverse AAAAAACTGCAG-TCAGTTCGCACTTTTTTCTGTTTTGGA Pst l 586 Forward CGCGGATCCCATATG-GCAGCCCATCTCG BamHI- Ndel Reverse CCCGCTCGAG-TTTCAGCGAATCAAGTTTC Xhol 587 Forward CGCGGATCCCATATG-GACCTGCCCTTGACGA BamHI- Ndel Reverse CCCGCTCGAG-AAATGTATGCTGTACGCC Xhol 588 Forward AAAAAAGAATTC-GCCGTCCTGACTTCCTATCAAGAACCAGG Eco RI Reverse AAAAAACTGCAG-TTATTTGTTTTTGGGCAGTTTCACTTC Pst l 589 Forward AAAAAAGAATTC- Eco RI ATGCAACAAAAAATCCGTTTCCAAATCGAAGG Reverse AAAAAACTGCAG-CTAATCGATTTTTACCCGTTTCAGGCG Pst l 590 Forward AAAAAAGAATTC-ATGAAAAAACCTTTGATTTCAGTTGCGGC Eco RI Reverse AAAAAACTGCAG-TTACTGCTGCGGCTCTGAAACCAT Pst l 591 Forward AAAAAAGAATTC-CACTACATCGTTGCCAGATTGTGCGG Eco RI Reverse AAAAAACTGCAG-CTAACCGAGCAGCCGGGTAACGTCGTT Pst l 592a Forward AAAAAAGAATTC-CGCGATTACACCGCCAAGCTGAAAATGGG Eco RI Reverse AAAAAACTGCAG-TTACCAAACGTCGGATTTGATACG Pst l 593 Forward CGCGGATCCGCTAGC-CTTGAACTGAACGGACTC BamHI- Nhel Reverse CCCGCTCGAG-GCGGAAGCGGACGATT Xhol 594a Forward AAAAAAGAATTC-GGTAAGTTCGCCGTTCAGGCCTTTCA Eco RI Reverse AAAAAACTGCAG-TTACGCCGCCGTTTCCTGACACTCGCG Pst l 595 Forward AAAAAAGAATTC-TGCCAGCCGCCGGAGGCGGAGAAAGC Eco RI Reverse AAAAAACTGCAG-TTATTTCAAGCCGAGTATGCCGCG Pst l 596 Forward CGCGGATCCCATATG-TCCCAACAATACGTC BamHI- Ndel Reverse CCCGCTCGAG-ACGCGTTACCGGTTTGT Xhol 597 Forward CGCGGATCCCATATG-CTGCTTCATGTCAGC BamHI- Ndel Reverse GCCCAAGCTT-ACGTATCCAGCTCGAAG Hindlll 601 Forward CGCGGATCCCATATG-ATATGTTCCCAACCGGCAAT BamHI- Ndel Reverse CCCGCTCGAG-AAAACAATCCTCAGGCAC Xhol 602 Forward CGCGGATCCGCTAGC-TTGCTCCATCAATGC BamHI- Nhel Reverse CCCGCTCGAG-ATGCAGCTGCTAAAAGCG Xhol 603 Forward AAAAAAGAATTC-CTGTCCTCGCGTAGGCGGGGACGGGG Eco RI Reverse AAAAAACTGCAG-CTACAAGATGCCGGCAAGTTCGGC Pst l 604 Forward CGCGGATCCGCTAGC-CCCGAAGCGCACTT BamHI- Nhel Reverse CCCGCTCGAG-GACGGCATCTGCACGG Xhol 606a Forward AAAAAAGAATTC-CGCGAATACCGCGCCGATGCGGGCGC Eco RI Reverse AAAAAACTGCAG-TTAAAGCGATTTGAGGCGGGCGATACG Pst l 607 Forward AAAAAAGAATTC-ATGCTGCTCGACCTCAACCGCTTTTC Eco RI Reverse AAAAAACTGCAG-TCAGACGGCCTTATGCGATCTGAC Pst l 608 Forward AAAAAAGAATTC-ATGTCCGCCCTCCTCCCCATCATCAACCG Eco RI Reverse AAAAAACTGCAG-TTAGTCTATCCAAATGTCGCGTTC Pst l 609 Forward CGCGGATCCCATATG-GTTGTGGATAGACTCG BamHI- Ndel Reverse CCCGCTCGAG-CTGGATTATGATGTCTGTC Xhol 610 Forward CGCGGATCCCATATG-ATTGGAGGGCTTATGCA BamHI- Ndel Reverse CCCGCTCGAG-ACGCTTCAACATCTTTGCC Xhol 611 Forward CGCGGATCCCATATG-CCGTCTCAAAACGGG BamHI- Ndel Reverse CCCGCTCGAG-AACGACTTTGAACGCGCAA Xhol 613 Forward CGCGGATCCCATATG-TCGCGTTCGAGCCG3 BamHI- Ndel Reverse CCCGCTCGAG-AGCCTGTAAAATAAGCGGC Xhol 614 Forward CGCGGATCCCATATG-TCCGTCGTGAGCGGC BamHI- Ndel Reverse CCCGCTCGAG-CCATACTGCGGCGTTC Xhol 616 Forward AAAAAAGAATTC-ATGTCAAACACAATCAAAATGGTTGTCGG Eco RI Reverse AAAAAATCTAGA-TTAGTCCGGGCGGCAGGCAGCTCG Xba l 619a Forward AAAAAAGAATTC-GGGCTTCTCGCCGCCTCGCTTGC Eco RI Reverse AAAAAACTGCAG-TCATTTTTTGTGTTTTAAAACGAGATA Pst l 622 Forward CGCGGATCCCATATG-GCCGCCCTGCCTAAAG BamHI- Ndel Reverse CCCGCTCGAG-TTTGTCCAAATGATAAATCTG Xhol 624 Forward CGCGGATCCCATATG-TCCCCGCGCTTTTACCG BamHI- Ndel Reverse CCCGCTCGAG-AGATTCGGGCCTGCGC Xhol 625 Forward CGCGGATCCCATATG-TTTGCAACCAGGAAAATG BamHI- Ndel Reverse CCCGCTCGAG-CGGCAAAATTACCGCCTT Xhol 627a Forward AAAAAAGAATTC-AAAGCAGGCGAGGCAGGCGCGCTGGG Eco RI Reverse AAAAAACTGCAG- Pst l TTACGAATGAAACAGGGTACCCGTCATCAAGGC 628 Forward AAAAAAGGTACC-GCCTTACAAACATGGATTTTGCGTTC Kpn I Reverse AAAAAACTGCAG-CTACGCACCTGAAGCGCTGGCAAA Pst l 629a Forward AAAAAAGAATTC-GCCACCTTTATCGCGTATGAAAACGA Eco RI Reverse AAAAAACTGCAG-TTACAACACCGCCGTCCGGTTCAAACC Pst l 630a Forward AAAAAAGAATTC-GCGGCTTTGGGTATTTCTTTCGG Eco RI Reverse AAAAAACTGCAG-TTAGGAGACTTCGCCAATGGAGCCGGG Pst l 635 Forward AAAAAAGAATTC- Eco RI ATGACCCAGCGACGGGTCGGCAAGCAAAACCG Reverse AAAAAACTGCAG-TTAATCCACTATAATCCTGTTGCT Pst l 638 Forward AAAAAAGAATTC-ATGATTGGCGAAAAGTTTATCGTAGTTGG Eco RI Reverse AAAAAACTGCAG-TCACGAACCGATTATGCTGATCGG Pst l 639 Forward CGCGGATCCCATATG-ATGCTTTATTTTGTTCG BamHI- Ndel Reverse CCCGCTCGAG-ATCGCGGCTGCCGAC Xhol 642 Forward CGCGGATCCCATATG-CGGTATCCGCCGCAAT BamHl- Ndel Reverse CCCGCTCGAG-AGGATTGCGGGGCATTA Xhol 643 Forward CGCGGATCCCATATG-GCTTCGCCGTCGGCAG BamHI- Ndel Reverse CCCGCTCGAG-AACCGAAAAACAGACCGC Xhol 644 Forward AAAAAAGAATTC- Eco RI ATGCCGTCTGAAAGGTCGGCGGATTGTTGCCC Reverse AAAAAATCTAGA-CTACCCGCAATATCGGCAGTCCAATAT Pst l 645 Forward AAAAAAGAATTC-GTGGAACAGAGCAACACGTTAAATCG Eco RI Reverse AAAAAACTGCAG-CTACGAGGAAACCGAAGACCAGGCCGC Pst l 647 Forward AAAAAAGAATTC-ATGCAAAGGCTCGCCGCAGACGG Eco RI Reverse AAAAAACTGCAG-TTAGATTATCAGGGATATCCGGTAGAA Pst l 648 Forward AAAAAAGAATTC- Eco RI ATGAACAGGCGCGACGCGCGGATCGAACG Reverse AAAAAACTGCAG-TCAAGCTGTGTGCTGATTGAATGCGAC Pst l 649 Forward AAAAAAGAATTC-GGTACGTCAGAACCCGCCCACCG Eco RI Reverse AAAAAACTGCAG-TTAACGGCGGAAACTGCCGCCGTC Pst l 650 Forward AAAAAAGAATTC-ATGTCCAAACTCAAAACCATCGC Eco RI Reverse AAAAAACTGCAG-TCAGACGGCATGGCGGTCTGTTTT Pst l 652 Forward AAAAAAGGTACC- Kpn I GCTGCCGAAGACTCAGGCCTGCCGCTTTACCG Reverse AAAAAACTGCAG-TTATTTGCCCAGTTGGTAGAATGCGGC Pst l 653 Forward AAAAAAGAATTC-GCGGCTTTGCCGGTAATTTTCATCGG Eco RI Reverse AAAAAACTGCAG-CTATGCCGGTCTGGTTGCCGGCGGCGA Pst l 656a Forward AAAAAAGAATTC-CGGCCGACGTCGTTGCGTCCTAAGTC Eco RI Reverse AAAAAACTGCAG-CTACGATTTCGGCGATTTCCACATCGT Pst l 657 Forward AAAAAAGAATTC-GCAGAATTTGCCGACCGCCATTTGTGCGC Eco RI Reverse AAAAAACTGCAG-TTATAGGGACTGATGCAGTTTTTTTGC Pst l 658 Forward CGCGGATCCCATATG-GTGTCCGGAATTGTG BamHI- Ndel Reverse CCCGCTCGAG-GGCAGAATGTTTACCGTT Xhol 661 Forward AAAAAAGAATTC- Eco RI ATGCACATCGGCGGCTATTTTATCGACAACCC Reverse AAAAAACTGCAG-TCACGACGTGTCTGTTCGCCGTCGGGC Pst l 663 Forward CGCGGATCCCATATG-TGTATCGAGATGAAATT BamHI- Ndel Reverse CCCGCTCGAG-GTAAAAATCGGGGCTGC Xhol 664 Forward CGCGGATCCCATATG-GCGGCTGGCGCGGT BamHI- Ndel Reverse CCCGCTCGAG-AAATCGAGTTTTACACCAC Xhol 665 Forward AAAAAAGAATTC-ATGAAATGGGACGAAACGCGCTTCGG Eco RI Reverse AAAAAACTGCAG-TCAATCCAAAATTTTGCCGACGATTTC Pst l 666 Forward AAAAAAGAATTC-AACTCAGGCGAAGGAGTGCTTGTGGC Eco RI Reverse AAAAAATCTAGA-TCAGTTTAGGGATAGCAGGCGTAC Xba l 667 Forward AAAAAAGAATTC- Eco RI CCGCATCCGTTTGATTTCCATTTCGTATTCGTCCG Reverse AAAAAACTGCAG-TTAATGACACAATAGGCGCAAGTC Pst l 669 Forward AAAAAAGAATTC-ATGCGCCGCATCATTAAAAAACACCAGCC Eco RI Reverse AAAAAACTGCAG-TTACAGTATCCGTTTGATGTCGGC Pst l 670a Forward AAAAAAGAATTC-AAAAACGCTTCGGGCGTTTCGTCTTC Eco RI Reverse AAAAAACTGCAG- Pst l TTAGGAGCTTTTGGAACGCGTCGGACTGGC 671 Forward CGCGGATCCCATATG-ACCAGCAGGGTAAC BamHI- Ndel Reverse CCCGCTCGAG-AGCAACTATAAAAACGCAAG Xhol 672 Forward CGCGGATCCCATATG-AGGAAAATCCGCACC BamHI- Ndel Reverse CCCGCTCGAG-ACGGGATAGGCGGTTG Xhol 673 Forward AAAAAAGAATTC-ATGGATATTGAAACCTTCCTTGCAGG Eco RI Reverse AAAAAACTGCAG-CTACAAACCCAGCTCGCGCAGGAA Pst l 674 Forward AAAAAAGAATTC-ATGAAAACAGCCCGCCGCCGTTCCCG Eco RI Reverse AAAAAACTGCAG-TCAACGGCGTTTGGGCTCGTCGGG Pst l 675 Forward CGCGGATCCCATATG-AACACCATCGCCCC BamHI- Ndel Reverse CCCGCTCGAG-TTCTTCGTCTTCAAACTGT Xhol 677a Forward AAAAAAGAATTC-AGACGGCATTCCCGATCAGTCGATTTTGA Eco RI Reverse AAAAAACTGCAG-TTACGTATGCGCGAAATCGACCGCCGC Pst l 680 Forward CGCGGATCCGCTAGC-ACGAAGGGCAGTTCGG BamHI- Nhel Reverse CCCGCTCGAG-CATCAAAAACCTGCCGC Xhol 681 Forward AAAAAAGAATTC-ATGACGACGCCGATGGCAATCAGTGC Eco RI Reverse AAAAAACTGCAG-TTACCGTCTTCCGCAAAAAACAGC Pst l 683 Forward CGCGGATCCCATATG-TGCAGCACACCGGACAA BamHI- Ndel Reverse CCCGCTCGAG-GAGTTTTTTTCCGCATACG Xhol 684 Forward CGCGGATCCCATATG-TGCGGTACTGTGCAAAG BamHI- Ndel Reverse CCCGCTCGAG-CTCGACCATCTGTTGCG Xhol 685 Forward CGCGGATCCCATATG-TGTTTGCTTAATAATAAACATT BamHI- Ndel Reverse CCCGCTCGAG-CTTTTTCCCCGCCGCA Xhol 686 Forward CGCGGATCCCATATG-TGCGGCGGTTCGGAAG BamHI- Ndel Reverse CCCGCTCGAG-CATTCCGATTCTGATGAAG Xhol 687 Forward CGCGGATCCCATATG-TGCGACAGCAAAGTCCA BamHI- Ndel Reverse CCCGCTCGAG-CTGCGCGGCTTTTTGTT Xhol 690 Forward CGCGGATCCCATATG-TGTTCTCCGAGCAAAGAC BamHI- Ndel Reverse CCCGCTCGAG-TATTCGCCCCGTGTTTGG Xhol 691 Forward CGCGGATCCCATATG-GCCACGGCTTATATCCC BamHI- Ndel Reverse CCCGCTCGAG-TTTGAGGCAGGAAGAAAG Xhol 694 Forward CGCGGATCCCATATG-TTGGTTTCCGCATCCGG BamHI- Ndel Reverse CCCGCTCGAG-TCTGCGTCGGTGCGGT Xhol 695 Forward CGCGGATCCCATATG-TTGCCTCAAACTCGTCCG BamHI- Ndel Reverse CCCGCTCGAG-TCGTTTGCGCACGGCT Xhol 696 Forward CGCGGATCCCATATG-TTGGGTTGCCGGCAGG BamHI- Ndel Reverse CCCGCTCGAG-TTGATTGCCGCAATGATG Xhol 700a Forward AAAAAAGAATTC-GCATCGACAGACGGTGTGTCGTGGAC Eco RI Reverse AAAAAACTGCAG-TTACGCTACCGGCACGACTTCCAAACC Pst l 701 Forward CGCGGATCCCATATG-AAGACTTGTTTGGATACTTC BamHI- Ndel Reverse CCCGCTCGAG-TGCCGACAACAGCCTC Xhol 702 Forward AAAAAAGAATTC-ATGCCGTGTTCCAAAGCCAGTTGGATTTC Eco RI Reverse AAAAAACTGCAG-TTAACCCCATTCCACCCGGAGAACCGA Pst l 703 Forward CGCGGATCCGCTAGC-CAAACGCTGGCAACCG BamHI- Nhel Reverse CCCGCTCGAG-TTTTGCAGGTTTGATGTTTG Xhol 704a Forward AAAAAAGAATTC-GCTTCTACCGGTACGCTGGCGCG Eco RI Reverse AAAAAACTGCAG- Pst l TTAGTTTTGCCGGATAATATGGCGGGTGCG 707 Forward CGCGGATCCGCTAGC-GAAATTATTAACGATGCAGA BamHI- Nhel Reverse CCCGCTCGAG-GAAACTGTAATTCAAGTTGA Xhol 708 Forward CGCGGATCCGCTAGC-CCTTTTAAGCCATCCAAAA BamHI- Nhel Reverse CCCGCTCGAG-TTGACCGGTGAGGACG Xhol 710 Forward CGCGGATCCCATATG-GAAACCCACGAAAAAATC BamHI- Ndel Reverse CCCGCTCGAG-AACGGTTTCGGTCAG Xhol 714 Forward CGCGGATCCCATATG-AGCTATCAAGACATCTT BamHI- Ndel Reverse CCCGCTCGAG-GCGGTAGGTAAATCGGAT Xhol 716 Forward CGCGGATCCCATATG-GCCAACAAACCGGCAAG BamHI- Ndel Reverse CCCGCTCGAG-TTTAGAACCGCATTTGCC Xhol 718 Forward CGCGGATCCCATATG-GAGCCGATAATGGCAAA BamHI- Ndel Reverse CCCGCTCGAG-GGCGCGGGCATGGTCTTGTCC Xhol 720 Forward CGCGGATCCCATATG-AGCGGATGGCATACC BamHI- Ndel Reverse CCCGCTCGAG-TTTTGCATAGCTGTTGACCA Xhol 723 Forward CGCGGATCCCATATG-CGACCCAAGCCCC BamHI- Ndel Reverse CCCGCTCGAG-AATGCGAATCCGCCGCC Xhol 725 Forward CGCGGATCCCATATG-GTGCGCACGGTTAAA BamHI- Ndel Reverse CCCGCTCGAG-TTGCTTATCCTTAAGGGTTA Xhol 726 Forward CGCGGATCCCATATG-ACCATCTATTTCAAAAAC BamHI- Ndel Reverse CCCGCTCGAG-GCCGATGTTTAGCGTCC Xhol 728 Forward CGCGGATCCCATATG-TTTTGGCTGGGAACGGG BamHI- Ndel Reverse CCCGCTCGAG-GTGAGAAAGGTCGCGC Xhol 729 Forward CGCGGATCCCATATG-TGCACCATGATTCCCCA BamHI- Ndel Reverse GCCCAAGCTT-TTTGTCGGTTTGGGTATC Hindlll 731 Forward CGCGGATCCGCTAGC-GCCGTGCCGGAGG BamHI- Nhel Reverse CCCGCTCGAG-ACGGGCGCGGCAG Xhol 732 Forward CCGGAATTCTACATATG-TCGAAACCTGTTTTTAAGAA EcoRI- Ndel Reverse CCCGCTCGAG-CTTCTTATCTTTTTTATCTTTC Xhol 733 Forward CGCGGATCCCATATG-GCCTGCGGCGGCAA BamHI- Ndel Reverse CCCGCTCGAG-TCGCTTGCCTCCTTTAC Xhol 734 Forward CGCGGATCCCATATG-GCCGATACTTACGGCTAT BamHI- Ndel Reverse CCCGCTCGAG-TTTGAGATTTTGAATCAAAGAG Xhol 735 Forward CGCGGATCCCATATG-AAGCAGCAGGCGGTCA BamHI- Ndel Reverse CCCGCTCGAG-ATTTCCGTAGCCGAGGG Xhol 737 Forward CGCGGATCCCATATG-CACCACGACGGACACG BamHI- Ndel Reverse CCCGCTCGAG-GTCGTCGCGGCGGGA Xhol 739 Forward CGCGGATCCCATATG-GCAAAAAAACCGAACA BamHI- Ndel Reverse CCCGCTCGAG-GAAGAGTTTGTCGAGAATT Xhol 740 Forward CGCGGATCCCATATG-GCCAATCCGCCCGAAG BamHI- Ndel Reverse CCCGCTCGAG-AAACGCGCCAAAATAGTG Xhol 741 Forward CGCGGATCCCATATG-TGCAGCAGCGGAGGG BamHI- Ndel Reverse CCCGCTCGAG-TTGCTTGGCGGCAAGGC Xhol 743 Forward CGCGGATCCCATATG-GACGGTGTTGTGCCTGTT BamHI- Ndel Reverse CCCGCTCGAG-CTTACGGATCAAATTGACG Xhol 745 Forward CGCGGATCCCATATG-TTTTGGCAACTGACCG BamHI- Ndel Reverse CCCGCTCGAG-CAAATCAGATGCCTTTAGG Xhol 746 Forward CGCGGATCCCATATG-TCCGAAAACAAACAAAAC BamHI- Ndel Reverse CCCGCTCGAG-TTCATTCGTTACCTGACC Xhol 747 Forward CCGGAATTCTAGCTAGC-CTGACCCCTTGGG EcoRI- Nhel Reverse GCCCAAGCTT-TTTTGATTTTAATTGACTATAGAAC Hindlll 749 Forward CGCGGATCCCATATG-TGCCAGCCGCCG BamHI- Ndel Reverse CCCGCTCGAG-TTTCAAGCCGAGTATGC Xhol 750 Forward CGCGGATCCCATATG-TGTTCGCCCGAACCTG BamHI- Ndel Reverse CCCGCTCGAG-CTTTTTCCCCGCCGCAA Xhol 758 Forward CGCGGATCCCATATG-AACAATCTGACCGTGTT Ba HI- Ndel Reverse CCCGCTCGAG-TGGCTCAATCCTTTCTGC Xhol 759 Forward CGCGGATCCGCTAGC-CGCTTCACACACACCAC BamHl- Nhel Reverse CCCGCTCGAG-CCAGTTGTAGCCTATTTTG Xhol 763 Forward CGCGGATCCCATATG-CTGCCTGAAGCATGGCG BamHI- Ndel Reverse CCCGCTCGAG-TTCCGCAAATACCGTTTCC Xhol 764 Forward CGCGGATCCCATATG-TTTTTCTCCGCCCTGA BamHI- Ndel Reverse CCCGCTCGAG-TCGCTCCCTAAAGCTTTC Xhol 765 Forward CGCGGATCCCATATG-TTAAGATGCCGTCCG BamHI- Ndel Reverse CCCGCTCGAG-ACGCCGACGTTTTTTATTAA Xhol 767 Forward CGCGGATCCCATATG-CTGACGGAAGGGGAAG BamHl- Ndel Reverse CCCGCTCGAG-TTTCTGTACAGCAGGGG Xhol 768 Forward CGCGGATCCCATATG-GCCCCGCAAAAACCCG BamHI- Ndel Reverse CCCGCTCGAG-TTTCATCCCTTTTTTGAGC Xhol 770 Forward CGCGGATCCCATATG-TGCGGCAGCGGCGAA Bamffl- Ndel Reverse CCCGCTCGAG-GCGTTTGTCGAGATTTTC Xhol 771 Forward CGCGGATCCCATATG-TCCGTATATCGCACCTTC BamHI- Ndel Reverse CCCGCTCGAG-CGGTTCTTTAGGTTTGAG Xhol 772 Forward CGCGGATCCCATATG-TTTGCGGCGTTGGTGG BamHl- Ndel Reverse CCCGCTCGAG-CAATGCCGACATCAAACG Xhol 774 Forward CGCGGATCCCATATG-TCCGTTTCACCCGTTCC BamHI- Ndel Reverse CCCGCTCGAG-TCGTTTGCGCACGGCT Xhol 790 Forward CGCGGATCCCATATG-GCAAGAAGGTCAAAAAC BamHI- Ndel Reverse CCCGCTCGAG-GGCGTTGTTCGGATTTCG Xhol 900 Forward CGCGGATCCCATATG-CCGTCTGAAATGCCG BamHI- Ndel Reverse CCCGCTCGAG-ATATGGAAAAGTCTGTTGTC Xhol 901 Forward CGCGGATCCCATATG-CCCGATTTTTCGATG BamHI- Ndel Reverse CCCGCTCGAG-AAAATGGAACAATACCAGG Xhol 902 Forward. CCGGAATTCTACATATG-TTGCACTTTCAAAGGATAATC EcoRI- 2 Ndel Reverse CCCGCTCGAG-AAAAATGTACAATGGCGTAC Xhol 903 Forward CCGGAATTCTAGCTAGC-CAGCGTCAGCAGCACAT EcoRI- Nhel Reverse CCCGCTCGAG-GAAACTGTAATTCAAGTTGAA Xhol 904 Forward AAAAAAGGTACC-ATGATGCAGCACAATCGTTTC Kpn I Reverse AAACTGCAG-TTAATATCGATAGGTTATATG Pst l 04a Forward AAAAAAGAATTC-CGGCTCGGCATTGTGCAGATGTTGCA Eco RI Reverse AAACTGCAG-TTAATATCGATAGGTTATATG Pst l 905 Forward CGCGGATCCCATATG-AACAAAATATACCGCATC BamHI- Ndel Reverse CCCGCTCGAG-CCACTGATAACCGACAGAT Xhol 907 Forward CGCGGATCCCATATG-GGCGCGCAACGTGAG BamHI- Ndel Reverse CCCGCTCGAG-ACGCCACTGCCAGCG Xhol 908 Forward AAAGAATTC-GCAGAGTTAGTAGGCGTTAATAAAAATAC Eco RI Reverse AAACTGCAG-TTAATATGGTTTTGTCGTTCG Pst l 909 Forward CGCGGATCCCATATG-TGCGCGTGGGAAACTTAT BamHI- Ndel Reverse CCCGCTCGAG-TCGGTTTTGAAACTTTGGTTTT Xhol 910 Forward AAAGAATTC-GCATTTGCCGGCGACTCTGCCGAGCG Eco RI Reverse AAACTGCAG-TCAGCGATCGAGCTGCTCTTT Pst l 911 Forward AAAGAATTC-GCTTTCCGCGTGGCCGGCGGTGC Eco RI Reverse AAAAAACTGCAG-GTCGACTTATTCGGCGGCTTTTTCCGC Pst l 912 Forward AAAAAAGAATTC- Eco RI CAAATCCGTCAAAACGCCACTCAAGTATTGAG Reverse AAAAAACTGCAG-TTACAGTCCGTCCACGCCTTTCGC Pst l 913 Forward CGCGGATCCCATATG-GAAACCCGCCCCGC BamHI- Ndel Reverse CCCGCTCGAG-AGGTTGTGTTCCAGGTTG Xhol 915 Forward CGCGGATCCCATATG-TGCCGGCAGGCGGAA BamHI- Ndel Reverse CCCGCTCGAG-TTTGAAAATATAGGTATCAGG Xhol 914 Forward AAAGAATTC-GACAGAATCGGCGATTTGGAAGCACG Eco RI Reverse AAACTGCAG-CTATATGCGCGGCAGGACGCTCAACGG Pst l 916 Forward CGCGGATCCCATATG-GCAATGATGGCGGCTG BamHI- Ndel Reverse CCCGCTCGAG-TTTGGCGGCATCTTTCAT Xhol 917 Forward AAAAAAGAATTC-CCTGCCGAAAAACCGGCACCGGC Eco RI Reverse AAAAAACTGCAG-TTATTTCCCCGCCTTCACATCCTG Pst l 919 Forward CGCGGATCCCATATG-TGCCAAAGCAAGAGCATC BamHI- Ndel Reverse CCCGCTCGAG-CGGGCGGTATTCGGG Xhol 920 Forward CGCGGATCCCATATG-CACCGCGTCTGGGTC BamHI- Ndel Reverse CCCGCTCGAG-ATGGTGCGAATGACCGA Xhol 921 Forward AAAAAAGAATTC-TTGACGGAAATCCCCGTGAATCC Eco RI Reverse AAAAAACTGCAG-TCATTTCAAGGGCTGCATCTTCAT Pst l 922 Forward. CGCGGATCCGCTAGC-TGTACGGCGATGGAGGC BamHI- 2 Nhel Reverse CCCGCTCGAG-CAATCCCGGGCCGCC Xhol 923 Forward CGCGGATCCCATATG-TGTTACGCAATATTGTCCC BamHI- Nhel Reverse CCCGCTCGAG-GGACAAGGCGACGAAG Xhol 925 Forward CGCGGATCCCATATG-AAACAAATGCTTTTAGCCG BamHI- Ndel Reverse CCCGCTCGAG-GCCGTTGCATTTGATTTC Xhol 926 Forward CGCGGATCCCATATG-TGCGCGCAATTACCTC BamHI- Ndel Reverse CCCGCTCGAG-TCTCGTGCGCGCCG Xhol 927 Forward CGCGGATCCCATATG-TGCAGCCCCGCAGC BamHI- Ndel Reverse CCCGCTCGAG-GTTTTTTGCTGACGTAGT Xhol 29a Forward AAAAAAGAATTC-CGCGGTTTGCTCAAAACAGGGCTGGG Eco RI Reverse AAAAAATCTAGA-TTAAGAAAGACGGAAACTACTGCC Xba l 931 Forward AAAAAAGAATTC-GCAACCCATGTTTTGATGGAAAC Eco RI Reverse AAAAAACTGCAG-TTACTGCCCGACAACAACGCGACG Pst l 935 Forward AAAAAAGAATTC- Eco RI GCGGATGCGCCCGCGATTTTGGATGACAAGGC Reverse AAAAAACTGCAG-TCAAAACCGCCAATCCGCCGACAC Pst l 936 Forward CGCGGATCCCATATG-GCCGCCGTCGGCGC BamHI- Ndel Reverse CCCGCTCGAG-GCGTTGGACGTAGTTTTG Xhol 937 Forward AAAAAAGAATTC-CCGGTTTACATTCAAACCGGCGCAAC Eco RI Reverse AAAAAACTGCAG-TTAAAATGTATGCTGTACGCCAAA Pst l 39a Forward AAAAAAGAATTC-GGTTCGGCAGCTGTGATGAAACC Eco RI Reverse AAAAAACTGCAG-TTAACGCAAACCTTGGATAAAGTTGGC Pst l 950 Forward CGCGGATCCCATATG-GCCAACAAACCGGCAAG BamHI- Ndel Reverse CCCGCTCGAG-TTTAGAACCGCATTTGCC Xhol 953 Forward CGCGGATCCCATATG-GCCACCTACAAAGTGGAC BamHI- Ndel Reverse CCCGCTCGAG-TTGTTTGGCTGCCTCGAT Xhol 957 Forward CGCGGATCCCATATG-TTTTGGCTGGGAACGGG BamHI- Ndel Reverse CCCGCTCGAG-GTGAGAAAGGTCGCGC Xhol 958 Forward CGCGGATCCCATATG-GCCGATGCCGTTGCG BamHI- Ndel Reverse GCCCAAGCTT-GGGTCGTTTGTTGCGTC Hindlll 959 Forward CGCGGATCCCATATG-CACCACGACGGACACG BamHI- Ndel Reverse CCCGCTCGAG-GTCGTCGCGGCGGGA Xhol 961 Forward CGCGGATCCCATATG-GCCACAAGCGACGACG BamHI- Ndel Reverse CCCGCTCGAG-CCACTCGTAATTGACGC Xhol 972 Forward AAAAAAGAATTC- Eco RI TTGACTAACAGGGGGGGAGCGAAATTAAAAAC Reverse AAAAAATCTAGA-TTAAAAATAATCATAATCTACATTTTG Xba l 973 Forward AAAAAAGAATTC-ATGGACGGCGCACAACCGAAAAC Eco RI Reverse AAAAAACTGCAG-TTACTTCACGCGGGTCGCCATCAGCGT Pst l 982 Forward CGCGGATCCCATATG-GCAGCAAAAGACGTAC BamHI- Ndel Reverse CCCGCTCGAG-CATCATGCCGCCCATCC Xhol 983 Forward CGCGGATCCCATATG-TTAGCTGTTGCAACAACAC BamHI- Ndel Reverse CCCGCTCGAG-GAACCGGTAGCCTACG Xhol 987 Forward CGCGGATCCCATATG-CCCCCACTGGAAGAAC BamHI- Ndel Reverse CCCGCTCGAG-TAATAAACCTTCTATGGGC Xhol 988 Forward CGCGGATCCCATATG-TCTTTAAATTTACGGGAAAAAG BamHI- Ndel Reverse GCCCAAGCTT-TGATTTGCCTTTCCGTTTT Hindlll 989 Forward CCGGAATTCTACATATG-GTCCACGCATCCGGCTA EcoRI- Ndel Reverse CCCGCTCGAG-TTTGAATTTGTAGGTGTATTGC Xhol 990 Forward. CGCGGATCCGCTAGC-TTCAGAGCTCAGCTT BamHI- 2 Nhel Reverse CCCGCTCGAG-AAACAGCCATTTGAGCGA Xhol 992 Forward CGCGGATCCCATATG-GACGCGCCCGCCCG BamHI- Ndel Reverse CCCGCTCGAG-CCAAATGCCCAACCATTC Xhol 993 Forward CGCGGATCCCATATG-GCAATGCTGATTGAAATCA BamHI- Ndel Reverse CCCGCTCGAG-GAACACATCGCGCCCG Xhol 996 Forward CGCGGATCCCATATG-TGCGGCAGAAAATCCGC BamHI- Ndel Reverse CCCGCTCGAG-TCTAAACCCCTGTTTTCTC Xhol 997 Forward CCGGAATTCTAGCTAGC-CGGCACGCCGACGTT EcoRI- Nhel Reverse CCCGCTCGAG-GACGGCATCGCTCAGG Xhol Underlined sequences indicate restriction recognition sites. The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1>: gOOl.seq 1 ATGCTGCCGC AGGGGAAGGC GGCGCGGAGG GTGTCGGCGA ACGAGGTGTC 51 CGGCAGGGCT TGCGCCCGGA TGGTGCTGGT CATCTGCCAG ACGCTGCCGA 101 AACGCGATAC TTTAAACGGC TCGGGTACGC ATACTTTACC GGTTTGGGCG 151 ATTTTGCCGA GGTCGTTGCG CAGCAAATCG ACAATCATCA CGTTTTCGGC 201 GCGGTTTTTC GGGTCGGTTT GTAACTCGGC GGCGCGGCGT TCGTCTTGTC 251 CGTCGCCCAA AATCGGCGCG GTGCCTTTCA TCGGTTCGGT GCTGATGGTG 301 CCGTCTGAAG CGATGTTGAG GAAGAGTTCG GGCGAGAAAC ACAGCGTCCA 351 CGCGGATTGC CCGGCTTCAT CGGGCAGGTG GGACAATACG GCATAG This corresponds to the amino acid sequence <SEQ ID 2; ORF 001. ng>: gOOl.pep 1 MLPQGKAARR VSAΝEVSGRA CARMVLVICQ TLPKRDTLΝG SGTHTLPV A 51 ILPRSLRSKS TIITFSARFF GSVCΝSAARR SSCPSPKIGA VPFIGSVLMV 101 PSEAMLRKSS GEKHSVHADC PASSGRWDΝT A* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 3>: mOOl . seq 1 ATGCTGCCGC AGGGGAAGGC GGCGCGGAGG ATGTCGGCGA ACGAGGTGTG 51 CGGcAssCTT ss.GCTTGGA yGGTGCTGGT CATCTGCCAA ACGCTGCCGA 101 AACGCGATAC TTTAAACGGT TCGGGTACGC ATACTGTGCC GGTTTGGGCG 151 ATTTTGCCGA GATCGTTACG CAGCAAATCG ACAATCATCA CGTTTTCGGC 201 GCGGTTTTTC GGGTCTGCTT GCAACTCGGC GGCGCGGCGT TCGTCTTGTC 251 CGTCGCCCAA AATCGGCGCG GTGCCTTTCA TCGGTTCGGT GCTGATGGTG 301 CCGTCCGAAC CGATTTTGAG GAAGAGTTCG GGCGAGAAAC ACAGCGTCCA 351 CGCGGATTGC CCCTCCGCAT CGGGCAGGTG GGACAAGACG GCATAG This corresponds to the amino acid sequence <SEQ ID 4; ORF 001>: mOOl .pep 1 MLPQGKAARR MSAΝEVCGXL XAWXV VICQ TLPKRDTLΝG SGTHTVPV A 51 ILPRSLRSKS TIITFSARFF GSACΝSAARR SSCPSPKIGA VPFIGSVLMV 101 PSEPILRKSS GEKHSVHADC PSASGRWDKT A* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 5>: aOOl.seq 1 ATGCTGCCGC AGGGGAAGGC GGCGCGGAGG ATGTCGGCGA ACGAGGTGTG 51 CGGCAAGGCT TGGGCTTGGA TGGTGCTGGT CATCTGCCAA ACGCTGCCGA 101 AACGCGATAC TTTAAACGGT TCGGGTACGC ATACTGTGCC GGTTTGGGCG 151 ATTTTGCCGA GGTCGTTACG CAGCAAATCG ACAATCATCA CGTTTTCGGC 201 GCGGTTTTTC GGGTCTGCTT GCAACTCGGC GGCGCGGCGT TCGTCTTGTC 251 CGTCGCCCAA AATCGGCGCG GTGCCTTTCA TCGGTTCGGT GCTGATGGTG 301 CCGTCCGAAC CGATTTTGAG GAAGAGTTCG GGCGAGAAAC ACAGCGTCCA 351 CGCGGATTGC CCTTGTGCAT CGGGCAGGTG GGACAAAACG GCATAG This corresponds to the amino acid sequence <SEQ ID 6; ORF 001. a>: aOOl.pep 1 MLPQGKAARR MSAΝEVCGKA WAWMVLVICQ TLPKRDTLΝG SGTHTVPVWA 51 ILPRSLRSKS TIITFSARFF GSACΝSAARR SSCPSPKIGA VPFIGSVLMV 101 PSEPILRKSS GEKHSVHADC PCASGRWDKT A* mOOl/aOOl 96.2% identity over a 131 aa overlap 10 20 30 40 50 60 mOOl . pep MLP Q GKAARRMSAΝEVCGXLXAWXVLVICQTLPKRDTLΝGSGTHTVPVWAILPRSLRSKS I I M I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ! I I I I I aOOl.pep MLP Q GKAARRMSAΝEVCGKAWAWMVLVICQTLPKRDTLΝGSGTHTVPVWAILPRSLRSKS 10 20 30 40 50 60 70 80 90 100 110 120 OOl.pep TIITFSARFFGSACNSAARRSSCPSPKIGAVPFIGSVLMVPSEPILRKSSGEKHSVHADC I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I II I I I I II I I I I I I I I I I I I I I I I I aOOl . pep TIITFSARFFGSACNSAARRSSCPSPKIGAVPFIGSVLMVPSEPILRKSSGEKHSVHADC 70 80 90 100 110 120 130 mOOl.pep PSASGRWDKTAX I I I I I I I I I I I aOOl.pep PCASGRWDKTAX 130 Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 001 shows 89.3% identity over a 131 aa overlap with a predicted ORF (ORF OOl.ng) from N gonorrhoeae: mOOl/gOOl 10 20 30 40 50 60 mOOl.pep MLPQGKAARRMSAΝEVCGXLXAWXVLVICQTLPKRDTLΝGSGTHTVPVWAILPRSLRSKS 1111111111:11111 I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I gOOl MLPQGKAARRVSAΝEVSGRACARMVLVICQTLPKRDTLΝGSGTHTLPVWAILPRSLRSKS 10 20 30 40 50 60 70 80 90 100 110 120 mOOl . pep TIITFSARFFGSACΝSAARRSSCPSPKIGAVPFIGSVLMVPSEPILRKSSGEKHSVHADC I I I I I I I I I I II: I I I I I I I I I I I II I I I I I II I II I I I I I II : II I II I I I I II I I II gOOl TIITFSARFFGSVCΝSAARRSSCPSPKIGAVPFIGSVLMVPSEAMLRKSSGEKHSVHADC 70 80 90 100 110 120 130 mO01. pep PSASGRWDKTAX I :: I I I I I : I I I gOOl PASSGRWDΝTAX 130 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 7>: g003.seq 1 ATGGTCGTAT TCGTGGCTGA AGGCGTATTC GGTCGCGCTG TTTTGGGTCA 51 CTTGGTATTG CTCTTCGGTC AGGGTGCGTT TGAGTTCGGC GTCACTCGGT 101 TTTTTATACG TTGCCGCGTC GAAGCCTTTG CCTTGCGGTG CGGCTTTGGT 151 TTTGCCCGGC AGCGGTTCGT CGGCTTTGCG GATGTCGATG TGGCAGTAGC 201 CGTTGGGGTT TTTAATCAGG TAGTCCTGAT GGTATTCCTC GGCGTCGTAG 251 AAGTTTTTCA GCGGTTCGTT TTCAACAACG AGGGGCAGTT GGTATTTTTG 301 CTGCTCGCGT TTGAGGGCGG CGGCGATGAC GGCTTTTTCG GCGGGGTCGG 351 TGTAGTACAC GCCGCTGCGG TATTGCGTGC CGGTGTCGTT ACCCTGTTTG 401 TTGAGGCTGG TCGGATCAAC GACGCGGAAA TAATATTGCA GGATGTCGTC 451 CAGgCTGagt TTGTCGGCAT CGTaggtcac tTTGACGGTC TCGGCATGAC 501 CCGTATGGCG GTaggacact tctTCgtanc TcGGGtTTTC CGTGttGCCG 551 TTGGCgttac cGGATACCGC gtcaACCACG CCGTcgatgc gttggaAATa 601 ggCTTCCAAg ccccaaaagc agccgccggc gaagtaaatg gtgcccgtgt 651 tcatgattGC TGa This corresponds to the amino acid sequence <SEQ ID 8; ORF 003.ng>: g003.pep 1 MVVFVAEGVF GRAVLGHLVL LFGQGAFEFG VTRFFIRCRV EAFALRCGFG 51 FARQRFVGFA DVDVAVAVGV FΝQVVLMVFL GVVEVFQRFV FΝΝEGQLVFL 101 LLAFEGGGDD GFFGGVGVVH AAAVLRAGVV TLFVEAGRIΝ DAEIILQDVV 151 QAEFVGIVGH FDGLGMTRMA VGHFFVRVFR VAVGVTGYRV NHAVDALEIG 201 FQAPKAAAGE VNGARVHDC The following partial DNA sequence was identified in N meningitidis <SEQ ID 9>: m003.seq 1 ATGGTCGTAT TCGTGGCTGA AGGCATATTC GGTCGCGCTG TTTTGGGTAA 51 CTTGsTATTG CTCTTCGGTC AGGGTGCGTT TGAGTTCGGC GTCACTCGGT 101 TTTTTATACG TTGCCGCGTC GAAGCCTTTG CCTTGCGGGG CGGTCTTGGT 151 TTTGCCCGGC AGCGGTTCGT CAGCkTTGCG GATGTCGATG TGGCAGTAGC 201 CGTTGGGGTT TTTAATCAAG TAGTCCTGAT GGTATTCCTC GGCATCGTAG 251 AAGTTTTtCA GCGGCTCGTT TTCAACAACG AGGGGCAGTT GGTATTTTTG 301 CTGCTCGCGT TTGAGGGCGk CGGCGATGAC GGCTTTTTCG kCGGGGTCGG 351 TGTAGTACAC GCCGCTGCGG TATTGCGTAC CGGTGTCGTT GCCCTGTTTG 401 TTGAGGCTGG TCGGATCAAC GACGCGGAAG AAATATTGCA GGATGTCGTC 451 TAGGCTGAGT TTGTCGGCAT CGTAGGTCAC TTTGACGGTT TCGGCGTGGC 501 CCGTATGGCG GTAGGACACG TCTTCATAGC TCGGATTTTT CGTGTTGCCG 551 TTGGCGTAGC CGGATACCGC GTCAACCACG CCGTCGATGC GTTGGAAATA 601 GGCTTCCAAG CCCCAGAAGC AGCg . CCGGC GAGGTAAATG GTGCGCGTGT 651 TCATGATTTT TGA This corresponds to the amino acid sequence <SEQ ID 10; ORF 003>: m003.ρep Length: 221 1 MVVFVAEGIF GRAVLGΝLXL LFGQGAFEFG VTRFFIRCRV EAFALRGGLG 51 FARQRFVSXA DVDVAVAVGV FΝQVVLMVFL GIVEVFQRLV FΝΝEGQLVFL 101 LLAFEGXGDD GFFXGVGVVH AAAVLRTGVV ALFVEAGRIΝ DAEEILQDVV 151 *AEFVGIVGH FDGFGVARMA VGHVFIARIF RVAVGVAGYR VΝHAVDALEI 201 GFQAPEAAXG EVΝGARVHDF * The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 11>: a003.seq 1 ATGGTCGTAT TCGTGGCTGA AGGCATATTC GGTCGCGCTG TTTTGGGTAA 51 CTTGGTATTG CTCTTCGGTC AGGGTGCGTT TGAGTTCGGC GTCACTCGGT 101 TTTTTATACG TTGCCGCGTC GAAGCCTTTG CCTTGCGGTG CGGTCTTGGT 151 TTTGCCCGGC AGCGGTTCGT CGGCTTTGCG GATATCGATG TGGCAGTAGC 201 CGTTGGGGTT TTTAATCAAG TAGTCCTGAT GGTATTCCTC GGCATCGTAG 251 AAGTTTTTCA GCGGCTCGTT TTCAACAACG AGGGGCAGTT GGTATTTTTG 301 CTGCTCGCGT TTGAGGGCGG CGGCGATGAC GGCTTTTTCG GCGGGGTCGG 351 TGTAGTACAC GCCGCTGCGG TATTGCGTAC CGGTGTCGTT GCCCTGTTTG 401 TTGAGGCTGG TCGGATCAAC GACGCGGAAG AAATATTGCA GGATGTCGTC 451 TAGGCTGAGT TTGTCGGCAT CGTAGGTCAC TTTGACGGTT TCGGCGTGGC 501 CCGTATGGCG GTAGGACACG TCTTCATAGC TCGGATTTTT CGTGTTGCCG 551 TTGGCGTAGC CGGATACCGC GTCAACCACG CCGTCGATGC GTTGGAAATA 601 GGCTTCCAAG CCCCAGAAGC AGCCGCCGGC GAGGTAGATG GTGCGCGTGT 651 TCATGATTTT TGA This corresponds to the amino acid sequence <SEQ ID 12; ORF 003. a>: a003.pep 1 MVVFVAEGIF GRAVLGΝLVL LFGQGAFEFG VTRFFIRCRV EAFALRCGLG 51 FARQRFVGFA DIDVAVAVGV FΝQVVLMVFL GIVEVFQRLV FΝΝEGQLVFL 101 LLAFEGGGDD GFFGGVGVVH AAAVLRTGVV ALFVEAGRIΝ DAEEILQDVV 151 *AEFVGIVGH FDGFGVARMA VGHVFIARIF RVAVGVAGYR VΝHAVDALEI 201 GFQAPEAAAG EVDGARVHDF * m003/a003 95.9% identity over a 220 aa overlap 10 20 30 40 50 60 m003 . pep MWFVAEGIFGRAVLGΝLXLLFGQGAFEFGVTRFFIRCRVEAFALRGGLGFARQRFVSXA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I a003 MVVFVAEGIFGRAVLGΝLVLLFGQGAFEFGVTRFFIRCRVEAFALRCGLGFARQRFVGFA 10 20 30 40 50 60 70 80 90 100 110 120 m003. pep DVDVAVAVGVFNQVVLMVFLGIVEVFQRLVFNNEGQLVFLLLAFEGXGDDGFFXGVGWH I : I I I I I I I I I I I II I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I llllll llllll a003 DIDVAVAVGVFNQVVLMVFLGIVEVFQRLVFNNEGQLVFLLLAFEGGGDDGFFGGVGWH 70 80 90 100 110 120 130 140 150 160 170 180 m003. pep AAAVLRTGVVALFVEAGRINDAEEILQDVVXAEFVGIVGHFDGFGVARMAVGHVFIARIF I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I a003 AAAVLRTGVVALFVEAGRINDAEEILQDWXAEFVGIVGHFDGFGVARMAVGHVFIARIF 130 140 150 160 170 180 190 200 210 220 m003. pep RVAVGVAGYRVNHAVDALEIGFQAPEAAXGEVNGARVHDFX I I I I I I I I I I I I I I I I I I I I I I I I I I I I 111:11111111 a003 RVAVGVAGYRVNHAVDALEIGFQAPEAAAGEVDGARVHDFX 190 200 210 220 Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 003 shows 88.6% identity over a 219 aa overlap with a predicted ORF (ORF 003.ng) from N gonorrhoeae: m003/g003 10 20 30 40 50 60 m003. pep MVVFVAEGIFGRAVLGΝLXLLFGQGAFEFGVTRFFIRCRVEAFALRGGLGFARQRFVSXA I I I I I I I I : I I I I I I I: I I I I I I I I I I I I I I I I I I I I I I I I I I I I 1:11111111: I g003 MVVFVAEGVFGRAVLGHLVLLFGQGAFEFGVTRFFIRCRVEAFALRCGFGFARQRFVGFA 10 20 30 40 50 60 70 80 90 100 110 120 m003.pep DVDVAVAVGVFΝQVVLMVFLGIVEVFQRLVFΝΝEGQLVFLLLAFEGXGDDGFFXGVGWH I I I I I I I I I I I I I I I I I I I I I : I I I I I I : I I I I I I I I I I I I I I I I I llllll llllll g003 DVDVAVAVGVFΝQVVLMVFLGVVEVFQRFVFΝΝEGQLVFLLLAFEGGGDDGFFGGVGVVH 70 80 90 100 110 120 130 140 150 160 170 180 m003. pep AAAVLRTGWALFVEAGRIΝDAEEILQDWXAEFVGIVGHFDGFGVARMAVGHVFIARIF I I I I I I : I I I : I I I I I I I I I I I I llllll I I I I I I I I I I I I : I : : I I I I I I I : I : I g003 AAAVLRAGWTLFVEAGRIΝDAEIILQDWQAEFVGIVGHFDGLGMTRMAVGHFFV-RVF 130 140 150 160 170 180 190 200 210 220 m003. pep RVAVGVAGYRVΝHAVDALEIGFQAPEAAXGEVΝGARVHDFX I I I I I I : I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I g003 RVAVGVTGYRVΝHAVDALEIGFQAPKAAAGEVΝGARVHDC 190 200 210 The following partial DΝA sequence was identified in N. gonorrhoeae <SEQ ID 13>: g004.seq 1 ATGgtagAAC GGCATATCCA GCATTTGCGG AACGGTCATC TTCATTTGAT 51 GCGCCCATGC CAACAagtga gccaAAtgtT CGGCGGCAGG GCCTacgatT 101 TCCGCGCCGA TAAagcggcc gGTGgctTTT tcgGCataca ggcgcaTatg 151 gCCTTTGTTT ACCAgcatca cgcggctgcg accttgaTTT TTGAACGATA 201 CTTCGCCgaT GACAAATTCG TCGGCTTGGT ATTGCGCGGC AACCTGCGCG 251 TATTTCAAAC CGACAAAGCC GATTTGCgga ctggtaaACA CCACGCCAAT 301 GGTgctgcgg cGCAAACCGC TGCCGATATt cgGgtagcgg ccccgcgtta 351 ttgcccggca atcttacctt ggtcggcggc ttcatGCAGC AGGGGCagtt 401 ggttggacgc gtcgcccgca ataAAGATAT GCGGAATgct ggtCTGCATg 451 gtCAGCGGAT CGGCAACGGG tacgccgcgc gcgtctttgT CGATATTGAT 501 GTTTTCCAAA CCGATATtgT CAACGTTCGG ACGGCgACCT ACGGCTGCCA 551 ACATATATTC GGCAACAAAT ACGCCTTTTT CGCCATCCTG CTCCCAATGG 601 ACTtctACAT TGCCGTCTGC GTCGAGTTTG ACCTCGGTTT TAGCATCCAG 651 ATGCAGTTTC AATtctTCTC CGAACACGGC TTTCGCCTCG TCTGAAACAA 701 CGGGGTCGGA AATGCCGCCG ATGATTCCGC CCAAACCGAA AATTTCAACT 751 TTCACACCCA AACGGTGCAA TGCCTGA This corresponds to the amino acid sequence <SEQ ID 14; ORF 004.ng>: g004.pep 1 MVERHIQHLR NGHLHLMRPC QQVSQMFGGR AYDFRADKAA GGFFGIQAHM 51 AFVYQHHAAA TLIFERYFAD DKFVGLVLRG NLRVFQTDKA DLRTGKHHAN 101 GAAAQTAADI RVAAPRYCPA ILPWSAASCS RGSWLDASPA IKICGMLVCM 151 VSGSATGTPR ASLSILMFSK PILSTFGRRP TAANIYSATN TPFSPSCSQW 201 TSTLPSASSL TSVLASRCSF NSSPNTAFAS SETTGSEMPP MIPPKPKIST 251 FTPKRCNA* The following partial DNA sequence was identified in N meningitidis <SEQ ID 15>: m004.seq 1 ATGGTAGAAC GGCATATCCA GCATTTGCGG AACGGTCATC TTCATTTGAT 51 GTGCCCAAGC CAACAGGTGC GCCAAATGTT CGGCGGCAGG GCCTACGATT 101 TCCGCGCCGA TAAAGCGGCC GGTGGCTTTT TCGGCATACA GGCGCATATG 151 GCCTTTGTTC ACCAGCATCA CGCGGCTGCG GCCTTGGTTT TTGAACGATA 201 CTTCGCCGAT GACAAATTCG TCGGCTTGGT ATTGCGCGGC AACCTGCGCG 251 TATTTCAGAC CGACAAAGCC GATTTGCGGA CTGGTAAACA CCACGCCGAT 301 GGTGCTGCGC CGCAAACCGC CGCCGATATT CGGGTAGCGG CCGCGTTATC 351 GCCGGCAATC TTGCCTTGGT CGGCAGCTTC ATGCAGCAGA GGCAGTTGGT 401 TGGACGCATC GCCTGCGATG AAGATATGCG GAATACTGGT CTGCATGGTC 451 AGCGGGTCGG CAACAGGTAC GCCGCGCGCA TCTTTTTCGA TATTGATATT 501 TTCCAAACCG ATATTGTCAA CGTTCGGACG GCGGCCCACG GCTGCCAGCA 551 TATATTCGGC AACAAATACG CCTTTTTCGC CATCCTGCTC CCAATGGACT 601 TCTACATTGC CGTCTGCATC GAGTTTGACC TCGGTTTTAG CATCCAGATG 651 CAGTTTCAAT TCTTCGCCGA ACACGGCGTT CGCCTCGTCT GAAACGACGG 701 GGTCGGAAAT GCCGCCGATG ATTCCGCCCA AACCGAAAAT TTCAACTTTC 751 ACGCCCAAAC GGTGCAATGC CTGA This corresponds to the amino acid sequence <SEQ ID 16; ORF 004>: m004.pep 1 MVERHIQHLR ΝGHLHLMCPS QQVRQMFGGR AYDFRADKAA GGFFGIQAHM 51 AFVHQHHAAA ALVFERYFAD DKFVGLVLRG NLRVFQTDKA DLRTGKHHAD 101 GAAPQTAADI RVAAALSPAI LPWSAASCSR GSWLDASPAM KICGILVCMV 151 SGSATGTPRA SFSILIFSKP ILSTFGRRPT AASIYSATNT PFSPSCSQWT 201 STLPSASSLT SVLASRCSFN SSPNTAFASS ETTGSEMPPM IPPKPKISTF 251 TPKRCNA* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 17>: a004. seq 1 ATGGTAGAAC GGCATATCCA GCATTTGCGG AACGGTCATC TTCATTTGAT 51 GTGCCCAAGC CAACAGGTGC GCCAAATGTT CGGCGGCCGG ACCTACGATT 101 TCTGCGCCGA TGAAGCGGCC GGTGGCTTTT TCGGCATACA GGCGCATATG 151 GCCTTTGTTT ACCAGCATCA CGCGGCTGCG GCCTTGGTTT TTGAACGATA 201 CTTCGCCGAT GACAAATTCG TCGGCTTGGT ATTGCGCGGC AACCTGCGCG 251 TATTTCAAAC CGACAAAGCC GATTTGCGGA CTGGTGAACA CTACGCCGAT 301 GGTGCTGCGG CGCAAACCGC CGCCGATATT CGGGTAGCGG CCGCGTTATC 351 GCCGGCAATC TTGCCTTGGT CGGCGGCTTC ATGCAGCAGG GGCAGTTGGT 401 TGGACGCGTC GCCCGCAATA AAGATATGCG GAATACTGGT CTGCATAGTC 451 AGCGGATCGG CAACGGGTAC GCCGCGCGCA TCTTTTTCGA TATTGATGTT 501 TTCCAAACCG ATATTGTCAA CGTTCGGACG GCGGCCTACG GCTGCCAGCA 551 TATATTCGGC AACAAATACG CCTTTTTCGC CATCCTGCTC CCAATGGACT 601 TCTACATTGC CGTCTGCGTC GAGTTTGGCC TCGGTTTTAG CATCCAAATG 651 CAGTTTCAAT TCTTCACCGA ACACGGCTTT CGCCTCGTCT GAAACGACGG 701 GGTCGGAAAT GCCGCCGATG ATGCCACCCA AACCGAAAAT TTCAACTTTC 751 ACGCCCAAAC GGTGCAATGC CTGA This corresponds to the amino acid sequence <SEQ ID 18; ORF 004. a>: a00 .pep 1 MVERHIQHLR NGHLHLMCPS QQVRQMFGGR TYDFCADEAA GGFFGIQAHM 51 AFVYQHHAAA ALVFERYFAD DKFVGLVLRG NLRVFQTDKA DLRTGEHYAD 101 GAAAQTAADI RVAAALSPAI LPWSAASCSR GSWLDASPAI KICGILVCIV 151 SGSATGTPRA SFSILMFSKP ILSTFGRRPT AASIYSATNT PFSPSCSQWT 201 STLPSASSLA SVLASKCSFN SSPNTAFASS ETTGSEMPPM MPPKPKISTF 251 TPKRCNA* m004/a004 94.9%> identity over a 257 aa overlap 10 20 30 40 50 60 m004.pep MVERHIQHLRNGHLHLMCPSQQVRQMFGGRAYDFRADKAAGGFFGIQAHMAFVHQHHAAA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I : I I I I I I I I I I I I I I I : I I I I I I a004 MVERHIQHLRNGHLHLMCPSQQVRQMFGGRTYDFCADEAAGGFFGIQAHMAFVYQHHAAA 10 20 30 40 50 60 70 80 90 100 110 120 m004.pep ALVFERYFADDKFVGLVLRGNLRVFQTDKADLRTGKHHADGAAPQTAADIRVAAALSPAI I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I : I I I I I I I I I I I I I I I I I I I I I a004 ALVFERYFADDKFVGLVLRGNLRVFQTDKADLRTGEHYADGAAAQTAADIRVAAALSPAI 70 80 90 100 110 120 130 140 150 160 170 180 m004.pep LPWSAASCSRGSWLDASPAMKICGILVCMVSGSATGTPRASFSILIFSKPILSTFGRRPT I I I I I I I I I I I I I I I I I I I : I I I I I II I : I I I I I I I I I I I I I I I I : I I I I I I I I II I I I I a004 LPWSAASCSRGSWLDASPAIKICGILVCIVSGSATGTPRASFSILMFSKPILSTFGRRPT 130 140 150 160 170 180 190 200 210 220 230 240 m004.pep AASIYSATNTPFSPSCSQWTSTLPSASSLTSVLASRCSFNSSPNTAFASSETTGSEMPPM I I I I I I I I I I I II I I I II I I I I I I I I I I I : I I I I I : I I II I I I I I I I I I I I I I I I I I I I I a004 AASIYSATNTPFSPSCSQWTSTLPSASSLASVLASKCSFNSSPNTAFASSETTGSEMPPM 190 200 210 220 230 240 250 m004.pep IPPKPKISTFTPKRCNAX : I I I I I I I I I I I I I I I I I a004 MPPKPKISTFTPKRCNAX 250 Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N. gonorrhoeae ORF 004 shows 93.4% identity over a 258 aa overlap with a predicted ORF (ORF 004.ng) from N. gonorrhoeae: m004/g004 10 20 30 40 50 60 m00 . pep MVERHIQHLRΝGHLHLMCPSQQVRQMFGGRAYDFRADKAAGGFFGIQAHMAFVHQHHAAA I I I I I I I I I I I I I I I I I I III I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I g004 MVERHIQHLRΝGHLHLMRPCQQVSQMFGGRAYDFRADKAAGGFFGIQAHMAFVYQHHAAA 10 20 30 40 50 60 70 80 90 100 110 119 m004. pep ALVFERYFADDKFVGLVLRGΝLRVFQTDKADLRTGKHHADGAAPQTAADIRVAAA-LSPA : I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I II I I II g004 TLIFERYFADDKFVGLVLRGΝLRVFQTDKADLRTGKHHAΝGAAAQTAADIRVAAPRYCPA 70 80 90 100 110 120 120 130 140 150 160 170 179 m004.pep ILPWSAASCSRGSWLDASPAMKICGILVCMVSGSATGTPRASFSILIFSKPILSTFGRRP I I I I I I I I I I I I I I I I I I I I : I I I I : I I I I I I I I I I I I I II I : I I I : I I I I I I I I I I I I I g 004 ILPWSAASCSRGSWLDASPAIKICGMLVCMVSGSATGTPRASLSILMFSKPILSTFGRRP 130 140 150 160 170 180 180 190 200 210 220 230 239 m004.pep TAASIYSATNTPFSPSCSQWTSTLPSASSLTSVLASRCSFNSSPNTAFASSETTGSEMPP I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I g004 TAANIYSATNTPFSPSCSQWTSTLPSASSLTSVLASRCSFNSSPNTAFASSETTGSEMPP 190 200 210 220 230 240 240 250 m004.pep MIPPKPKISTFTPKRCNAX I I I I I I I I I I I I I I I I I I g004 MIPPKPKISTFTPKRCNA 250 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 19>: g005. seq 1 ATGGGGATGG ACAATATTGA TATGTTCATG CCTGAACAAG AGGAAATCCA 51 ATCAATGTGG AAAGAAATTT TACTGAATTA CGGTATTTTC CTGCTCGAAC 101 TGCTTACCGT GTTCGGCGCA ATTGCGCTGA TTGTGTTGGC TATCGTACAG 151 AGTAAGAAAC AGTCGGAAAG CGGCAGTGTC GTACTGACAG ATTTTTCGGA 201 AAATTATAAA AAACAGCGGC AATCGTTTGA AACATTCTTT TTAAGCGAGG 251 AAGAGACAAA ACATCAGGAA AAAAAAGAAA AGAAAAAGGA AAAGGCGGAA 301 GCCAAAGCAG AGAAAAAGCG TTTGAAGGAG GGCGGGGAGA AATCTGCCGA 351 AACGCAAAAA TCCCGCCTTT TTGTGTTGGA TTTTGACGGC GATTTGTATG 401 CACACGCCGT AGAATCCTTG CGTCATGAGA TTACGGCGGT GCTTTTGATT 451 GCCAAGCCTG AAGATGAGGT TCTGCTCAGA TTGGAAAGTC CGGGCGGCGT 501 GGTTCACGGT TACGGTTTGG CGGCTTCGCA GCTTAGGCGT TTGCGCGAAC 551 GCAATATTCC GCTGAccgtc gccgTCGATA AGGTCGCGGC AAGCGgcggc 601 tatatgatgg cgtgtgtgGC GGATAAAATT GTTTCCGCtc cgtttgcggt 651 catcggttcg gtgggtgtgg tgGcggaagt gcCGAATATC CAccgCctGT 701 TGAAAAAACA TGATATTGAT GTGGATGTGA TGACGGCGGG CGAATTTAAG 751 CGCACGGTTA CTTTTATGGG TGAAAATACG GAAAAGGGCA AACAGAAATT 801 CCGGCAGGAA CTGGAGGAAA CGCATCAGTT GTTCAAGCAG TTTGTCAGTG 851 AAAACCGCCC CGGGTTGGAT ATTGAAAAAA TAGCGACGGG CGAGCATTGG 901 TTCGGCCGGC AGGCGTTGGC GTTGAACTTG ATTGACGAGA TTTCGACCAG 951 TGATGATTTG TTGTTGAAAG CGTTTGAAAA CAAACAGGtt aTCGAAGTGA 1001 AATATCAGGA GAAGCGAAGC CTGATCCAGC GCATTGGTTT GCAGGCGGAA 1051 GCTTCCGTTG AAAAGTTGTT TGCCAAACTT GTCAACCGGC GAGCGGATGT 1101 GATGTAG This corresponds to the amino acid sequence <SEQ ID 20; ORF 005.ng>: g005.pep 1 MGMDΝIDMFM PEQEEIQSMW KEILLΝYGIF LLELLTVFGA IALIVLAIVQ 51 SKKQSESGSV VLTDFSEΝYK KQRQSFETFF LSEEETKHQE KKEKKKEKAE 101 AKAEKKRLKE GGEKSAETQK SRLFVLDFDG DLYAHAVESL RHEITAVLLI 151 AKPEDEVLLR LESPGGVVHG YGLAASQLRR LRERΝIPLTV AVDKVAASGG 201 YMMACVADKI VSAPFAVIGS VGVVAEVPΝI HRLLKKHDID VDVMTAGEFK 251 RTVTFMGEΝT EKGKQKFRQE LEETHQLFKQ FVSEΝRPGLD IEKIATGEHW 301 FGRQALALΝL IDEISTSDDL LLKAFEΝKQV IEVKYQEKRS LIQRIGLQAE 351 ASVEKLFAKL VΝRRADVM* The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 21>: m005. seq 1 ATGGACAATA TTGACATGTT CATGCCTGAA CAAGAGGAAA TCCAATCAAT 51 GTGGAAAGAA ATTTTACTGA ATTACGGTAT TTTCCTGCTC GAACTGCTTA 101 CCGTGTTCGG CGCAATTGCG CTGATTGTGT TGGCTATCGT ACAGAGTAAG 151 AAACAGTCGG A AGCGGCAG TGTCGTACTG ACGGATTTTT CGGAAAATTA 201 TAAAAAACAG CGGCAATCGT TTGAAGCATT CTTTTTAAGC GGGGAAGAGG 251 CACAACATCA GGAAAAAGAG GAAAAGAAAA AGGAAAAGGC GGAAGCCAAA 301 GCAGAGAAAA A.CGTTTGAA GGAGGGTGGG GAGAAATCTG CCGAAACGCA 351 nAAATCACGC CTTTTTGTGT TGGANNNNNN NNNNNNNNNN NNNNNNNNNN 401 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 451 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 501 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN 551 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNGCGAGCGG CGGTTATATG 601 ATGGCGTGTG TGGCGGATAA AATTGCTTCC GCTCCGTTTG CGATTGTCGG 651 TTCGGTGGGT GTGGTGGCGG AAGTACCGAA TATCCACCGC CTGTTGAAAA 701 AACATGATAT TGATGTGGAT GTGATGACGG CGGGCGAATT TAAGCGCACG 751 GTTACTTTTA TGGGTGAAAA TACGGAAAAG GGCAAACAGA AATTCCGACA 801 GGAACTGGAG GAAACGCATC AGTTGTTCAA GCAGTTTGTC AGCGAGAACC 851 GCCCTCAATT GGATATTGAG GAAGTGGCAA CGGGCGAGCA TTGGTTCGGT 901 CGGCAGGCGT TGGCGTTGAA CTTGATTGAC GAGATTTCGA CCAGTGATGA 951 TTTGTTGTTG AAAGCGTTTG AAAACAAACA GGTTATCGAA GTGAAATATC 1001 AGGAGAAGCA AAGCCTGATC CAGCGCATTG GTTTGCAGGC GGAAGCTTCT 1051 GTTGAAAAGT TGTTTGCCAA ACTTGTCAAC CGGCGGGCGG ATGTGATGT A 1101 G This corresponds to the amino acid sequence <SEQ ID 22; ORF 005>: m005.pep 1 MDNIDMFMPE QEEIQSMWKE ILLNYGIFLL ELLTVFGAIA LIVLAIVQSK 51 KQSXSGSVVL TDFSENYKKQ RQSFEAFFLS GEEAQHQEKE EKKKEKAEAK 101 AEKXRLKEGG EKSAETXKSR LFVLXXXXXX XXXXXXXXXX XXXXXXXXXX 151 XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXASGGYM 201 MACVADKIAS APFAIVGSVG VVAEVPNIHR LLKKHDIDVD VMTAGEFKRT 251 VTFMGENTEK GKQKFRQELE ETHQLFKQFV SENRPQLDIE EVATGEHWFG 301 RQALALNLID EISTSDDLLL KAFENKQVIE VKYQEKQSLI QRIGLQAEAS 351 VEKLFAKLVN RRADVM* The following partial DNA sequence was identified in N meningitidis <SEQ ID 23>: a005.seq 1 ATGGACAATA TTGACATGTT CATGCCTGAA CAAGAGGAAA TCCAATCAAT 51 GTGGAAAGAA ATTTTACTGA ATTACGGTAT TTTCCTGCTC GAACTGCTTA 101 CCGTGTTCGG CGCAATTGCG CTGATTGTGT TGGCTATCGT ACAGAGTAAG 151 AAACAGTCGG AAAGCGGCAG TGTCGTACTG ACGGATTTTT CGGAAAATTA 201 TAAAAAACAG CGGCAATCGT TTGAAGCATT CTTTTTAAGC GGGGAAGAGG 251 CAAAACATCA GGAAAAAGAG GAAAAGAAAA AGGAAAAGGC GGAAGCCAAA 301 GCAGAGAAAA AGCGTTTGAA GGAGGGTGGG GAGAAATCTT CCGAAACGCA 351 AAAATCCCGC CTTTTTGTGT TGGATTTTGA CGGCGATTTG TATGCACACG 401 CCGTAGAATC CTTGCGTCAT GAGATTACGG CGGTGCTTTT GATTGCCAAG 451 CCTGAAGATG AGGTTCTGCT TAGATTGGAA AGTCCGGGCG GCGTGGTTCA 501 CGGTTACGGT TTGGCGGCTT CGCAGCTTAG GCGTTTGCGC GAACGCAATA 551 TTCCGCTGAC CGTCGCCGTC GATAAGGTGG CGGCGAGCGG TGGTTATATG 601 ATGGCGTGTG TGGCGGATAA AATTGTTTCC GCTCCGTTTG CGATTGTCGG 651 TTCGGTGGGT GTTGTAGCGG AAGTACCGAA TATCCACCGC CTGTTGAAAA 701 AACATGATAT TGATGTGGAT GTGATGACGG CGGGCGAATT TAAGCGCACG 751 GTTACTTTTA TGGGTGAAAA TACGGAAAAG GGCAAACAGA AATTCCGACA 801 GGAACTGGAG GAAACGCATC AGTTGTTCAA GCAGTTTGTC AGCGAGAACC 851 GCCCTCAATT GGATATTGAG GAAGTGGCAA CGGGCGAGCA TTGGTTCGGT 901 CGGCAGGCGT TGGCGTTGAA CTTGATTGAC GAGATTTCGA CCAGTGATGA 951 TTTGTTGTTG AAAGCGTTTG AAAACAAACA GGTTATCGAA GTGAAATATC 1001 AGGAGAAGCA AAGCCTGATC CAGCGCATTG GTTTGCAGGC GGAAGCTTCT 1051 GTTGAAAAGT TGTTTGCCAA ACTTGTCAAC CGGCGGGCGG ATGTGATGTA 1101 G This corresponds to the amino acid sequence <SEQ ID 24; ORF 005. a>: a005.pep 1 MDNIDMFMPE QEEIQSMWKE ILLNYGIFLL ELLTVFGAIA LIVLAIVQSK 51 KQSESGSVVL TDFSENYKKQ RQSFEAFFLS GEEAKHQEKE EKKKEKAEAK 101 AEKKRLKEGG EKSSETQKSR LFVLDFDGDL YAHAVESLRH EITAVLLIAK 151 PEDEVLLRLE SPGGVVHGYG LAASQLRRLR ERNIPLTVAV DKVAASGGYM 201 MACVADKIVS APFAIVGSVG VVAEVPNIHR LLKKHDIDVD VMTAGEFKRT 251 VTFMGENTEK GKQKFRQELE ETHQLFKQFV SENRPQLDIE EVATGEHWFG 301 RQALALNLID EISTSDDLLL KAFENKQVIE VKYQEKQSLI QRIGLQAEAS 351 VEKLFAKLVN RRADVM* tι005/a005 79.2%> identity over a 366 aa overlap 10 20 30 40 50 60 m005. pep MDNIDMFMPEQEEIQSMWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSXSGSWL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I a005 MDNIDMFMPEQEEIQSMWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSWL 10 20 30 40 50 60 70 80 90 100 110 120 m005. pep TDFSENYKKQRQSFEAFFLSGEEAQHQEKEEKKKEKAEAKAEKXRLKEGGEKSAETXKSR I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I 111111111:11 III a005 TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSSETQKSR 70 80 90 100 110 120 130 140 150 160 170 180 mO05. pep LFVLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX I I I I : a005 LFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQLRRLR 130 140 150 160 170 180 190 200 210 220 230 240 m005. pep XXXXXXXXXXXXXXASGGYMMACVADKIASAPFAIVGSVGVVAEVPNIHRLLKKHDIDVD : I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a005 ERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKKHDIDVD 190 200 210 220 230 240 250 260 270 280 290 300 m005. pep VMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEVATGEHWFG I I I I I I I I I I I I I I I I I M I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I a005 VMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEVATGEHWFG 250 260 270 280 290 300 310 320 330 340 350 360 m005.pep RQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEASVEKLFAKLVN I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I a005 RQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEASVEKLFAKLVN 310 320 330 340 350 360 m005.pep RRADVMX a005 RRADVMX Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 005 shows 77.0% identity over a 366 aa overlap with a predicted ORF (ORF 005. ng) from N gonorrhoeae: m005/g005 10 20 30 40 50 m005.pep MDΝIDMFMPEQEEIQSMWKEILLΝYGIFLLELLTVFGAIALIVLAIVQSKKQSXSGSV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g005 MGMDΝIDMFMPEQEEIQSMWKEILLΝYGIFLLELLTVFGAIALIVLAIVQSKKQΞESGSV 10 20 30 40 50 60 60 70 80 90 100 110 m005.pep VLTDFSEΝYKKQRQSFEAFFLSGEEAQHQEKEEKKKEKAEAKAEKKRLKEGGEKSAETXK I I I I I I I I I I I I I I I I I : I I I I I I : : I I I I : I I I I I I I I I I I I I I I I I I I I I 1 I I I I I g005 VLTDFSEΝYKKQRQSFETFFLSEEETKHQEKKEKKKEKAEAKAEKKRLKEGGEKSAETQK 70 80 90 100 110 120 120 130 140 150 160 170 O05. pep SRLFVLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX : g005 SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQLRR 130 140 150 160 170 180 180 190 200 210 220 230 m005. pep XXXXXXXXXXXXXXXXASGGYMMACVADKIASAPFAIVGSVGVVAEVPNIHRLLKKHDID : I I I I I II I I I I I I I : I I I I I : : I I I I I I I I I I I I I I I I I I I I I I g005 LRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAVIGSVGVVAEVPNIHRLLKKHDID 190 200 210 220 230 240 240 250 260 270 280 290 m005. pep VDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEVATGEHW I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I :: I I I I I I g005 VDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPGLDIEKIATGEHW 250 260 270 280 290 300 300 310 320 330 340 350 m005. pep FGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEASVEKLFAKL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I: I I I I I I I I I I I I I I I I I I I I I g005 FGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKRSLIQRIGLQAEASVEKLFAKL 310 320 330 340 350 360 360 O05. pep VNRRADVMX I I I I I I I I I g005 VNRRADVMX The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 25>: gOOβ.seq 1 ATGCTGCTGG TGCTggaatt ttggttCGGc gtGtCGGCGG TGGGCatact 51 tgCGTTGTTT TTATGGCttt TGCCACGTTT TGCCGCCATC AGCGAAAACC 101 TGTATTTCCG CCTGAACAAC AGCTTGGAAC gcgACAACCA CTTTATCCGA 151 AAAGGCGACG AGCGGCAGCT GTACCGCCAT TACGGACTGG TTTCGCGCCT 201 GCGTGTGCTG ATTTCCAACC GCGAAGCCTT CGGCTATCTC TGCGTCGGCG 251 CGGCGATGGG TATTTTGTTC GGCTTTGCTT TTGTGATGAT GACGCTCAAA 301 GGCTACGGCA GCGCGGGGCA TATTTATTCG GTCGGCACTT ATCTGTGGAT 351 GTTTGCCATG AGTTTGGACG ATGTGCCGCG ATTGGTCGAA CAATATTCCA 401 ATTTGAAAGA CATCGGACAA CGGATAGAGT GGTCGGAACG GAACATCAAA 451 GCCGGAACTT GA This corresponds to the amino acid sequence <SEQ ID 26; ORF 006.ng>: gOOβ.pep 1 MLLVLEFWFG VSAVGILALF LWLLPRFAAI SEΝLYFRLΝΝ SLERDΝHFIR 51 KGDERQLYRH YGLVSRLRVL ISΝREAFGYL CVGAAMGILF GFAFVMMTLK 101 GYGSAGHIYS VGTYLWMFAM SLDDVPRLVE QYSΝLKDIGQ RIEWSERΝIK 151 AGT* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 27>: 006.seq 1 ATGCTGCTGG TGCTGGAATT TTGGGTCGGC GTGTCGGCGG TGGGCATACT 51 TGCGTTGTTT TTATGGCTTT TGCCACGTTT TGCCGCCATC AGCGAAAACC 101 TGTATTTCCG CCTGAACAAC AGCTTGGAAC GCGACAACCA CTTTATCCGA 151 AAAGGCGACC GGCGGCAGCT GTACCGCCAT TACGGACTGC TTGCGCGCCT 201 GCGTGTGCTG ATTTCCAACC GCGAAGCCTT CGGCTATCTC TGCGTCGGCA 251 CGGCGATGGG TATTTTGTTC GGCTTTGCTT TTGTGATGAT GACGCTCAAA 301 GGCTACAGCA GCGCGGGGCA TGTCTATTCG GTCGGCACTT ATCTGTGGAT 351 GTTTGCCATG AGTTTGGACG ACGTGCCGCG ATTGGTCGAA CAATATTCCA 401 ATTTGAAAGA CATCGGACAA CGGATAGAGT GGTCGGAACG GAACATCAAA 451 GCCGGAACTTGA This corresponds to the amino acid sequence <SEQ ID 28; ORF 006>: mO06. ep 1 MLLVLEFWVG VSAVGILALF LWLLPRFAAI SENLYFRLNN SLERDNHFIR 51 KGDRRQLYRH YGLLARLRVL ISNREAFGYL CVGTAMGILF GFAFVMMTLK 101 GYSSAGHVYS VGTYLWMFAM SLDDVPRLVE QYSNLKDIGQ RIEWSERNIK 151 AGT* The following partial DNA sequence was identified in N meningitidis <SEQ ID 29>: a006.seq 1 ATGCTGCTGG TGCTGGAATT TTGGGTCGGC GTGTCGGCGG TGGGCATACT 51 TGCGTTGTTT TTATGGCTTT TGCCACGTTT TGCCGCCATC AGCGAAAACC 101 TGTATTTCCG CCTGAAGAAC AGCTTGGAAC GCGACAACCA CTTTATCCGA 151 AAAGGCGACG AGCGGCAGCT GGACCGCCAT TACGGACTGC TTGCGCGCCT 201 GCGTGTGCTG ATTTCCAACC GCGAAGCCTT CGGCTATCTC TGCGTCGGCA 251 CGGCGATGGG TATTTTGTTC GGCTTTGCTT TTGTGATGAT GACGCTCAAA 301 GGCTACAGCA GCGCGGGGCA TGTCTATTCG GTCGGCACTT ATCTGTGGAT 351 GTTTGCCATA AGTTTGGACG ACGTGCCGCG ATTGGTCGAA CAATATTCCA 401 ATTTGAAAGA CATCGGACAA CGGATAGAGT GGTCGAAACG GAACATCAAA 451 GCCGGAACTT GA This corresponds to the amino acid sequence <SEQ ID 30; ORF 006.a>: aOOδ.pep 1 MLLVLEFWVG VSAVGILALF LWLLPRFAAI SEΝLYFRLKΝ SLERDNHFIR 51 KGDERQLDRH YGLLARLRVL ISNREAFGYL CVGTAMGILF GFAFVMMTLK 101 GYSSAGHVYS VGTYLWMFAI SLDDVPRLVE QYSNLKDIGQ RIEWSKRNIK 151 AGT* moo6/a006 96.1% identity over a 153 aa overlap 10 20 30 40 50 60 m006.pep MLLVLEFWVGVSAVGILALFLWLLPRFAAISENLYFRLNNSLERDNHFIRKGDRRQLYRH I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I : I I I II a006 MLLVLEFWVGVSAVGILALFLWLLPRFAAISENLYFRLKNSLERDNHFIRKGDERQLDRH 10 20 30 40 50 60 70 80 90 100 110 120 mOOδ.pep YGLLARLRVLISNREAFGYLCVGTAMGILFGFAFVMMTLKGYSSAGHVYSVGTYLWMFAM I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I : a006 YGLLARLRVLISNREAFGYLCVGTAMGILFGFAFVMMTLKGYSSAGHVYSVGTYLWMFAI 70 80 90 100 110 120 130 140 150 m006.pep SLDDVPRLVEQYSNLKDIGQRIEWSERNIKAGTX I I I I I I I I I I I I I I I I I I I I I I I I I: I I I I I I I I a006 ΞLDDVPRLVEQYSNLKDIGQRIEWSKRNIKAGTX 130 140 150 Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 006 shows 95.4% identity over a 153 aa overlap with a predicted ORF (ORF OOό.ng) from N gonorrhoeae: m006/g006 10 20 30 40 50 60 m006.pep MLLVLEFWVGVSAVGILALFLWLLPRFAAISEΝLYFRLΝΝSLERDΝHFIRKGDRRQLYRH g006 MLLVLEFWFGVSAVGILALFLWLLPRFAAISENLYFRLNNSLERDNHFIRKGDERQLYRH 10 20 30 40 50 60 70 80 90 100 110 120 m006.pep YGLLARLRVLISNREAFGYLCVGTAMGILFGFAFVMMTLKGYSSAGHVYSVGTYLWMFAM l l | : : | I M I I I I I I I I I I I I I l l l l l l l l ! l l l l l l l l l l l l l l M I I I M I I g006 YGLVSRLRVLISNREAFGYLCVGAAMGILFGFAFVMMTLKGYGSAGHIYSVGTYLWMFAM 70 80 90 100 110 120 130 140 150 m006.pep SLDDVPRLVEQYSNLKDIGQRIEWSERNIKAGTX gll8 SLDDVPRLVEQYSNLKDIGQRIEWSERNIKAGT 130 140 150 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 31>: g006-l . seq 1 ATGTGGAAAA TGTTGAAACA CATAGCCAAA ACCCACCGCA AGCGATTGAT 51 TGGCACATTT TCCCCGGTCG GACTGGAAAA CCTTTTGATG CTGGGGTATC 101 CGGTGTTTGG CGGCTGGGCG ATTAATGCCG TGATTGCGGG GAGGGTGTGG 151 CAGGCGTTGC TGTACGCTTT GGTTGTATTT TTGATGTGGC TGGTCGGTGC 201 GGCACGGCGG ATTGCCGATA CGCGCACGTT TACGCGGATT TATACCGAAA 251 TCGCCGTGCC GGTTGTGTTG GAACAACGGC AGCGGCAAGT CCCGCATTCA 301 GCGGTAACTG CACGGGTTGC CCTGTCGCGT GAATTTGTCA GCTTTTTTGA 351 AGAACACCTG CCGATTGCCG CGACATCCGT CGTATCCATA TTCGGCGCGT 401 GCATCATGCT GCTGGTGCTG GAATTTTGGG TCGGCGTGTC GGCGGTGGGC 451 ATACTTGCGT TGTTTTTATG GCTTTTGCCA CGTTTTGCCG CCATCAGCGA 501 AAACCTGTAT TTCCGCCTGA ACAACAGCTT GGAACGCGAC AACCACTTTA 551 TCCGAAAAGG CGACGAGCGG CAGCTGTACC GCCATTACGG ACTGGTTTCG 601 CGCCTGCGTG TGCTGATTTC CAACCGCGAA GCCTTCGGCT ATCTCTGCGT 651 CGGCGCGGCG ATGGGTATTT TGTTCGGCTT TGCTTTTGTG ATGATGACGC 701 TCAAAGGCTA CGGCAGCGCG GGGCATATTT ATTCGGTCGG CACTTATCTG 751 TGGATGTTTG CCATGAGTTT GGACGATGTG CCGCGATTGG TCGAACAATA 801 TTCCAATTTG AAAGACATCG GACAACGGAT AGAGTGGTCG GAACGGAACA 851 TCAAAGCCGG AACTTGA This corresponds to the amino acid sequence <SEQ ID 32; ORF 006-l.ng>: g006-l .pep 1 MWKMLKHIAK THRKRLIGTF SPVGLEΝLLM LGYPVFGGWA IΝAVIAGRVW 51 QALLYALVVF LMWLVGAARR IADTRTFTRI YTEIAVPVVL EQRQRQVPHS 101 AVTARVALSR EFVSFFEEHL PIAATSVVSI FGACIMLLVL EFWVGVSAVG 151 ILALFLWLLP RFAAISEΝLY FRLΝΝSLERD ΝHFIRKGDER QLYRHYGLVS 201 RLRVLISΝRE AFGYLCVGAA MGILFGFAFV MMTLKGYGSA GHIYSVGTYL 251 WMFAMSLDDV PRLVEQYSΝL KDIGQRIEWS ERΝIKAGT* The following partial DΝA sequence was identified inN meningitidis <SEQ ID 33>: m006-l. seq 1 ATGTGGAAAA TGTTGAAACA CATAGCCCAA ACCCACCGCA AGCGATTGAT 51 TGGCACATTT TCCCTGGTCG GACTGGAAAA CCTTTTGATG CTGGTGTATC 101 CGGTGTTTGG CGGCCGGGCG ATCAATGCCG TGATTGCGGG GGAGGTGTGG 151 CAGGCGTTGC TGTACGCTTT GGTTGTGCTT TTGATGTGGC TGGTCGGTGC 201 GGTGCGGCGG ATTGCCGATA CGCGCACGTT TACGCGGATT TATACCGAAA 251 TCGCCGTGCC GGTCGTGTTG GAACAGCGGC AGCGACAAGT CCCGCATTCG 301 GCGGTAACTG CGCGGGTTGC CCTGTCGCGT GAGTTTGTCA GCTTTTTTGA 351 AGAACACCTG CCGATTGCCG CGACATCCGT CGTATCCATA TTCGGCGCGT 401 GCATCATGCT GCTGGTGCTG GAATTTTGGG TCGGCGTGTC GGCGGTGGGC 451 ATACTTGCGT TGTTTTTATG GCTTTTGCCA CGTTTTGCCG CCATCAGCGA 501 AAACCTGTAT TTCCGCCTGA ACAACAGCTT GGAACGCGAC AACCACTTTA 551 TCCGAAAAGG CGACCGGCGG CAGCTGTACC GCCATTACGG ACTGCTTGCG 601 CGCCTGCGTG TGCTGATTTC CAACCGCGAA GCCTTCGGCT ATCTCTGCGT 651 CGGCACGGCG ATGGGTATTT TGTTCGGCTT TGCTTTTGTG ATGATGACGC 701 TCAAAGGCTA CAGCAGCGCG GGGCATGTCT ATTCGGTCGG CACTTATCTG 751 TGGATGTTTG CCATGAGTTT GGACGACGTG CCGCGATTGG TCGAACAATA 801 TTCCAATTTG AAAGACATCG GACAACGGAT AGAGTGGTCG GAACGGAACA 851 TCAAAGCCGG AACTTGA This corresponds to the amino acid sequence <SEQ ID 34; ORF 006- 1>: m006-l.pep 1 MWKMLKHIAQ THRKRLIGTF SLVGLENLLM LVYPVFGGRA INAVIAGEVW 51 QALLYALVVL LMWLVGAVRR IADTRTFTRI YTEIAVPVVL EQRQRQVPHS 101 AVTARVALSR EFVSFFEEHL PIAATSVVSI FGACIMLLVL EFWVGVSAVG 151 I ALFLWLLP RFAAISENLY FRLNNSLERD NHFIRKGDRR QLYRHYGLLA 201 RLRVLISNRE AFGYLCVGTA MGILFGFAFV MMTLKGYSSA GHVYSVGTYL 251 WMFAMSLDDV PRLVEQYSNL KDIGQRIEWS ERNIKAGT* m006-ι/g006-ι 95.5% identity in 288 aa overlap 10 20 30 40 50 60 m006-l.pep MWKMLKHIAQTHRKRLIGTFSLVGLENLLMLVYPVFGGRAINAVIAGEVWQALLYALWL I I I II I I I I : I I I I I I I I I II I I I I I I I I I I I I I I I I I : I I I I I I I I I I I : g006-l MWKMLKHIAKTHRKRLIGTFSPVGLENLLMLGYPVFGGWAINAVIAGRVWQALLYALWF 10 20 30 40 50 60 70 80 90 100 110 120 m006-l.pep LMWLVGAVRRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAVTARVALSREFVSFFEEHL I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g006-l LMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAVTARVALSREFVSFFEEHL 70 80 90 100 110 120 130 140 150 160 170 180 m006-l . pep PIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLPRFAAISENLYFRLNNSLERD I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g006-l PIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLPRFAAISENLYFRLNNSLERD 130 140 150 160 170 180 190 200 210 220 230 240 m006-l.pep NHFIRKGDRRQLYRHYGLLARLRVLISNREAFGYLCVGTAMGILFGFAFVMMTLKGYSSA I I I I I I I I : I I I I I I I I I : I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I : I I g006-l NHFIRKGDERQLYRHYGLVSRLRVLISNREAFGYLCVGAAMGILFGFAFVMMTLKGYGSA 190 200 210 220 230 240 250 260 270 280 289 m006-l .pep GHVYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQRIEWSERNIKAGTX I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g006-l GHIYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQRIEWSERNIKAGTX 250 260 270 280 The following partial DNA sequence was identified in N meningitidis <SEQ ID 35>: a006-l.seq (partial) 1 ..AGCCAAAACC ACCGCAAGCG ATTGATTGGC ACATTTTTTC TGGTCGGACT 51 GGAAAACCTT TTGATGCTGG TGTATCCGGT GTTTGGCGGC TGGGCGATTA 101 ATGCCGTGAT TGCGGGGCAG GCGTGGCAGG CGTTGCTGTA CGCTTTGGTT 151 GTGCTTTTGA TGTGGCTGGT CGGTGCGGCG CGGCGGATTG CCGATACGCG 201 CACGTTTACG CGGATTTATA CCGAAATCGC CGTGCCGGTT GTGTTGGAAC 251 AGCGGCAGCG GCAAGTCCCG CATTCGGCGG TAACTGCGCG GGTTGCCCTG 301 TCGCGTGAGT TTGTCAGCTT TTTTGAAGAA CACCTGCCGA TTGCCGCGAC 351 ATCCGTCGTA TCCATATTCG GCGCGTGCAT CATGCTGCTG GTGCTGGAAT 401 TTTGGGTCGG CGTGTCGGCG GTGGGCATAC TTGCGTTGTT TTTATGGCTT 451 TTGCCACGTT TTGCCGCCAT CAGCGAAAAC CTGTATTTCC GCCTGAAGAA 501 CAGCTTGGAA CGCGACAACC ACTTTATCCG AAAAGGCGAC GAGCGGCAGC 551 TGGACCGCCA TTACGGACTG CTTGCGCGCC TGCGTGTGCT GATTTCCAAC 601 CGCGAAGCCT TCGGCTATCT CTGCGTCGGC ACGGCGATGG GTATTTTGTT 651 CGGCTTTGCT TTTGTGATGA TGACGCTCAA AGGCTACAGC AGCGCGGGGC 701 ATGTCTATTC GGTCGGCACT TATCTGTGGA TGTTTGCCAT AAGTTTGGAC 751 GACGTGCCGC GATTGGTCGA ACAATATTCC AATTTGAAAG ACATCGGACA 801 ACGGATAGAG TGGTCGAAAC GGAACATCAA AGCCGGAACT TGA This corresponds to the amino acid sequence <SEQ ID 36; ORF 006-1. a>: a006-l.pep (partial) 1 . .SQNHRKRLIG TFFLVGLENL LMLVYPVFGG WAINAVIAGQ AWQALLYALV 51 VLLMWLVGAA RRIADTRTFT RIYTEIAVPV VLEQRQRQVP HSAVTARVAL 101 SREFVSFFEE HLPIAATSW SIFGACIMLL VLEFWVGVSA VGILALFLWL 151 LPRFAAISEN LYFRLKNSLE RDNHFIRKGD ERQLDRHYGL LARLRVLISN 201 REAFGYLCVG TAMGILFGFA FVMMTLKGYS SAGHVYSVGT YLWMFAISLD 251 DVPRLVEQYS NLKDIGQRIE WSKRNIKAGT * a0O6-l/m006-l 95.7% identity in 280 aa overlap 10 20 30 40 50 a006-l.pep SQNHRKRLIGTFFLVGLENLLMLVYPVFGGWAINAVIAGQAWQALLYALWL : I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : : I I I I I I I I I I I m006-l MWKMLKHIAQTHRKRLIGTFSLVGLENLLMLVYPVFGGRAINAVIAGEVWQALLYALWL 10 20 30 40 50 60 60 70 80 90 100 110 a006-l . pep LMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAVTARVALSREFVSFFEEHL I I I I I I I: I I II I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m006-l LMWLVGAVRRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAVTARVALSREFVSFFEEHL 70 80 90 100 110 120 120 130 140 150 160 170 a006-l.pep PIAATSWSIFGACIMLLVLEFWVGVSAVGILALFLWLLPRFAAISENLYFRLKNSLERD I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I : I I I I I I m006-l PIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLPRFAAISENLYFRLNNSLERD 130 140 150 160 170 180 180 190 200 210 220 230 a006-l.pep NHFIRKGDERQLDRHYGLLARLRVLISNREAFGYLCVGTAMGILFGFAFVMMTLKGYSSA 11111111:111 I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m006-l NHFIRKGDRRQLYRHYGLLARLRVLISNREAFGYLCVGTAMGILFGFAFVMMTLKGYSSA 190 200 210 220 230 240 240 250 260 270 280 a006-l.pep GHVYSVGTYLWMFAIΞLDDVPRLVEQYSNLKDIGQRIEWSKRNIKAGTX I I I I I I I I I I I I I I : I I II I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I m006-l GHVYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQRIEWSERNIKAGTX 250 260 270 280 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 37>: g007.seq 1 atgaACACAA CCCGACTGCC GACCGCCTTC ATCTTGTGCT GCCTCTGcgC 51 CGCcGCTTCT GCCGccgaca acAGCatcat gaCaAAAGGG CAAAAAGTGT 101 ACGAATCcAa ctGCATCGCC TGCCACGGCA AGAAAGGGGA AGGGCGCGGC 151 ACTGCGtTTC CTccgctTTT CCggtcgGac tgtattatga acaAACCGCa 201 cgTCCtgctg cacagcatgg tcaaaggcAt cgacgggaca ttcaaagtgg 251 agcggcaaaa cctacgacgg atttatgCcc gcaaccgcca tcagcgATGC 301 GGACATTGCC GCCGTCGCCA CTTATATCAT GAACGCCTTT GA This corresponds to the amino acid sequence <SEQ ID 38; ORF 007.ng>: g007.pep 1 MΝTTRLPTAF ILCCLCAAAS AADΝSIMTKG QKVYESΝCIA CHGKKGEGRG 51 TAFPPLFRSD CIMΝKPHVLL HSMVKGIDGT FKVERQΝLRR IYARΝRHQRC 101 GHCRRRHLYH ERL* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 39>: m007 . seq 1 ATGAACACAA CCCGACTGCC GACCGCCCTC GTCTTGGGCT GCTTCTGCGC 51 CGCCGCTTCT GCCGCCGACA ACAGCATCAT GACAAAAGGG CAAAAAGTGT 101 ACGAATCCAA CTGCGTCGCC TGCCACGGCA AAAAGGGCGA AGGCCGCGGA 151 ACCATGTTTC CGCCGCTCTA CCGCTCCGAC TTCATCATGA AAAAACCGCA 201 GGTGCTGCTG CACAGCATGG TCAAAGGCAT CAACGGTACA ATCAAAGTC. 251 AACGGCAAAA CCTACAACGG ATTCATGCCC GCAACCGCCA TCAGCGATGC 301 GGACATTGCC GCCGTCGCCA CTTATATCAT GAACGCCTTT GA This corresponds to the amino acid sequence <SEQ ID 40; ORF 007>: m007.pep 1 MNTTRLPTAL VLGCFCAAAS AADNSIMTKG QKVYESNCVA CHGKKGEGRG 51 TMFPPLYRSD FIMKKPQVLL HSMVKGINGT IKVXRQNLQR IHARNRHQRC 101 GHCRRRHLYH ERL* The following partial DNA sequence was identified in N meningitidis <SEQ ID 41>: a007.seq 1 ATGAACACAA CCCGACTGCC GACCGCCCTC GTCTTGGGCT GCCTCTGCGC 51 CGCCGCTTCT GCCGCCGACA ACAGCATCAT GACAAAAGGG CAAAAAGTGT 101 ACGAATCCAA CTGCGTCGCC TGCCACGGCA AAAAGGGCGA AGGCCGCGGA 151 ACCATGTTTC CGCCGCTCTA CCGCTCCGAC TTCATCATGA AAAAACCGCA 201 GGTGCTGCTG CACAGCATGG TCAAAGGCAT CAACGGTACA ATCAAAGTC. 251 AACGGCAAAA CCTACAACGG ATTCATGCCC GCCACTGCCA TCAGCGATGC 301 GGACATTGCC GCCGTCGCCA CTTATATCAT GAACGCCTTT GA This corresponds to the amino acid sequence <SEQ ID 42; ORF 007.a>: a007.pep 1 MNTTRLPTAL VLGCLCAAAS AADNSIMTKG QKVYESNCVA CHGKKGEGRG 51 TMFPPLYRSD FIMKKPQVLL HSMVKGINGT IKVXRQNLQR IHARHCHQRC 101 GHCRRRHLYH ERL* m007/a007 91.3% identity over a 113 aa overlap 10 20 30 40 50 60 m007.pep MNTTRLPTALVLGCFCAAASAADNSIMTKGQKVYESNCVACHGKKGEGRGTMFPPLYRSD I I I I I I I I I I I I I I: I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I II I I a007 MNTTRLPTALVLGCLCAAASAADNSIMTKGQKVYESNCVACHGKKGEGRGTMFPPLYRSD 10 20 30 40 50 60 70 80 90 100 110 m007.pep FIMKKPQVLLHSMVKGINGTIKVXRQNLQRIHARNRHQRCGHCRRRHLYHERLX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I: I I I I I I I I I I I I I I I I I I a007 FIMKKPQVLLHSMVKGINGTIKVXRQNLQRIHARHCHQRCGHCRRRHLYHERLX 70 80 90 100 110 Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 007 shows 86.7% identity over a 113 aa overlap with a predicted ORF (ORF 007.ng) from N gonorrhoeae: m007/g007 10 20 30 40 50 60 m007.pep MΝTTRLPTALVLGCFCAAASAADΝSIMTKGQKVYESΝCVACHGKKGEGRGTMFPPLYRSD I I I I I I I I I:: I I: I I I I II I I I I I I I I II I I I I I II: I I I I I I I I I I I I 1111:111 g007 MΝTTRLPTAFILCCLCAAASAADΝSIMTKGQKVYESΝCIACHGKKGEGRGTAFPPLFRSD 10 20 30 40 50 60 70 80 90 100 110 m007. pep FIMKKPQVLLHSMVKGIΝGTIKVXRQΝLQRIHARΝRHQRCGHCRRRHLYHERLX I I: I I: I I I I I I I I I I : I I : I I I I I I : I I : I I I I I I I I I I I I I I I I I I I I I g007 CIMNKPHVLLHSMVKGIDGTFKVERQNLRRIYARNRHQRCGHCRRRHLYHERL 70 80 90 100 110 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 43>: g007-l.seq (partial) 1 ATGAACACAA CCCGACTGCC GACCGCCTTC ATCTTGTGCT GCCTCTGCGC 51 CGCCGCTTCT GCCGCCGACA ACAGCATCAT GACAAAAGGG CAAAAAGTGT 101 ACGAATCCAA CTGCATCGCC TGCCACGGCA AGAAAGGGGA AGGGCGCGGC 151 ACTGCGTTTC CTCCGCTTTT CCGGTCGGAC TATATTATGA ACAAACCGCA 201 CGTCCTGCTG CACAGCATGG TCAAAGGCAT CAACGGTACA ATCAAAGTCA 251 ACGGCAAAAC CTACAACGGA TTCATGCCCG CAACCGCCAT CAGCGATGCG 301 GACATTGCCG CCGTCGCCAC TTATATCATG AACGCCTTTG ACAACGGCGG 351 CGGAAGCGTT ACCGAAAAAG ACGTAAAACA GGCAAAAGGC AAAAAAAAC . This corresponds to the amino acid sequence <SEQ ID 44; ORF 007-l.ng>: g007-l.pep (partial) 1 MΝTTRLPTAF ILCCLCAAAS AADNSIMTKG QKVYESNCIA CHGKKGEGRG 51 TAFPPLFRSD YIMNKPHVLL HSMVKGINGT IKVNGKTYNG FMPATAISDA 101 DIAAVATYIM NAFDNGGGSV TEKDVKQAKG KKN... The following partial DNA sequence was identified in N meningitidis <SEQ ID 45>: m007-l.seq 1 ATGAACACAA CCCGACTGCC GACCGCCCTC GTCTTGGGCT GCTTCTGCGC 51 CGCCGCTTCT GCCGCCGACA ACAGCATCAT GACAAAAGGG CAAAAAGTGT 101 ACGAATCCAA CTGCGTCGCC TGCCACGGCA AAAAGGGCGA AGGCCGCGGA 151 ACCATGTTTC CGCCGCTCTA CCGCTCCGAC TTCATCATGA AAAAACCGCA 201 GGTGCTGCTG CACAGCATGG TCAAAGGCAT CAACGGTACA ATCAAAGTCA 251 ACGGCAAAAC CTACAACGGA TTCATGCCCG CAACCGCCAT CAGCGATGCG 301 GACATTGCCG CCGTCGCCAC TTATATCATG AACGCCTTTG ACAACGGCGG 351 CGGAAGCGTT ACCGAAAAAG ACGTAAAACA GGCAAAAAGC AAAAAAAACT 401 AA This corresponds to the amino acid sequence <SEQ ID 46; ORF 007- 1> m007-l .pep 1 MNTTRLPTAL VLGCFCAAAS AADNSIMTKG QKVYESNCVA CHGKKGEGRG 51 TMFPPLYRSD FIMKKPQVLL HSMVKGINGT IKVNGKTYNG FMPATAISDA 101 DIAAVATYIM NAFDNGGGSV TEKDVKQAKS KKN* m007-ι / g007-ι 91.7% identity in 133 aa overlap 10 20 30 40 50 60 m007-l . pep MNTTRLPTALVLGCFCAAASAADNSIMTKGQKVYESNCVACHGKKGEGRGTMFPPLYRSD I I I I I I I I I :: I I : I II I I I I I I I I I I I I I I I I I I I I : I I I II I I I I I I I 1111:111 g007-l MNTTRLPTAFILCCLCAAASAADNSIMTKGQKVYESNCIACHGKKGEGRGTAFPPLFRSD 10 20 30 40 50 60 70 80 90 100 110 120 m007-l.pep FIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADIAAVATYIMNAFDNGGGSV : I I: I I : I I I I I I II I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g007-l YIMNKPHVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADIAAVATYIMNAFDNGGGSV 70 80 90 100 110 120 130 mO07-1. pep TEKDVKQAKSKKNX I I I II I II I : I I I g007-l TEKDVKQAKGKKN 130 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 47>: a007-l.seq (partial) 1 ATGAACACAA CCCGACTGCC GACCGCCCTC GTCTTGGGCT GCCTCTGCGC 51 CGCCGCTTCT GCCGCCGACA ACAGCATCAT GACAAAAGGG CAAAAAGTGT 101 ACGAATCCAA CTGCGTCGCC TGCCACGGCA AAAAGGGCGA AGGCCGCGGA 151 ACCATGTTTC CGCCGCTCTA CCGCTCCGAC TTCATCATGA AAAAACCGCA 201 GGTGCTGCTG CACAGCATGG TCAAAGGCAT CAACGGTACA ATCAAAGTCA 251 ACGGCAAAAC CTACAACGGA TTCATGCCCG CCACTGCCAT CAGCGATGCG 301 GACATTGCCG CCGTCGCCAC TTATATCATG AACGCCTTTG ACAACGGCGG 351 CGGAAGCGTT ACCGAAAAAG ACGTAAAACA GGCAAAAAAC AAAAAA.. This corresponds to the amino acid sequence <SEQ ID 48; ORF 007-1. a>: a007-l.pep (partial) 1 MNTTRLPTAL VLGCLCAAAS AADNSIMTKG QKVYESNCVA CHGKKGEGRG 51 TMFPPLYRSD FIMKKPQVLL HSMVKGINGT IKVNGKTYNG FMPATAISDA 101 DIAAVATYIM NAFDNGGGSV TEKDVKQAKN KK.. moo7-i/aoo7-i 98.5% identity in 132 aa overlap 10 20 30 40 50 60 mO07-1. pep MNTTRLPTALVLGCFCAAASAADNSIMTKGQKVYESNCVACHGKKGEGRGTMFPPLYRSD I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I aO07-1 MNTTRLPTALVLGCLCAAASAADNSIMTKGQKVYESNCVACHGKKGEGRGTMFPPLYRSD 10 20 30 40 50 60 70 80 90 100 110 120 m007-l.pep FIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADIAAVATYIMNAFDNGGGSV I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I a007-l FIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADIAAVATYIMNAFDNGGGSV 70 80 90 100 110 120 130 m007-l.pep TEKDVKQAKSKKNX I I I I I I I I I : I I a007-l TEKDVKQAKNKK 130 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 49>: 1 ATGAACAACA GACATTTTGC CGTCAtcgCC TTGGGCAGCA ACCTTGACAA 51 CCCCGCACAA CAAATacgcg gcgcattaga cgcgctctcg tcccatcctg 101 acatccggct tgaaCaggtt tcctcactgt aTatgaccgc acctgtcggt 151 tacgAcaaTC agcccgATTT CATCaatgcc gTCTgcaccg TTTCCACCAC 201 CtTGGACGGC ATTGcccTGC TTGCCgaACT CAAccgTATC GAAGCCGATT 251 TCGGACGCGA aCGCAGTTTC CGCAATGCAC CGCGCACATT GGATTTGGAC 301 ATTATCGACT TTGACGGCAT CTCCAGCGAC GACCCCCGCC TTACCCTGCC 351 GCATCCGCGC GCGCACGAAC GCAGTTTCGT CATACGCCCT TTGGCAGAAA 401 TCCTCCCTGA TTTTATTTTG GGAAAATACG GAAAGGTTGT CGAATTGTCA 451 AAACGGCTGG GCAATCAAGG CATCCGTCTT TTACCGGACA GGTAA This corresponds to the amino acid sequence <SEQ ID 50; ORF 008.ng>: gOOΘ.pep 1 MΝΝRHFAVIA LGSΝLDΝPAQ QIRGALDALS SHPDIRLEQV SSLYMTAPVG 51 YDΝQPDFIΝA VCTVSTTLDG IALLAELΝRI EADFGRERSF RΝAPRTLDLD 101 IIDFDGISSD DPRLTLPHPR AHERSFVIRP LAEILPDFIL GKYGKVVELS 151 KRLGΝQGIRL LPDR* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 51>: mOOδ.seq 1 ATGAACAACA GACATTTTGC CGTCATCGCC CTGGGCAGTA ATCTTGAAAA 51 CCCTGCTCAA CAGGTACGCG CCGCATTGGA CACGCTGTCG TCCCATCCTG 101 ACATCCGTCT TAAACAGGCT TCCTCACTGT ATATGACCGC GCCCGTCGGT 151 TACGACAATC AGCCCGATTT TGTCAATGCC GTCTGCACCG TTTCCACCAC 201 TCTGGACGGC ATTGCCyTGC TTGCCGAACT CAACCGTATC GAGGCTGATT 251 TCGGACGCGA ACGCAGCTTC CGCAACGCGC CGCGCACATT GkATTTGGAC 301 ATTATCGACT TTGACGGCAT CTCCAGCGAC GACACsCGAC TcACCtTGCC 351 GCATCCGCGC GCGCACGAAC GCAGTTTCGT CATCCGCCCT TTGGCAGAAA 401 TCCTCCCTGA TTTTGTTTTA GGAAAACACG GAAAGGTTGC CGAATTGTCA 451 AAACGGyTGG GCAATCAAGG TATCCGTCTT TTACCGGACA GGTAATT This corresponds to the amino acid sequence <SEQ ID 52; ORF 008>: mOOβ.pep 1 MNNRHFAVIA LGSNLENPAQ QVRAALDTLS SHPDIRLKQA SSLYMTAPVG 51 YDNQPDFVNA VCTVSTTLDG IALLAELNRI EADFGRERSF RNAPRTLXLD 101 IIDFDGISSD DTRLTLPHPR AHERSFVIRP LAEILPDFVL GKHGKVAELS 151 KRLGNQGIRL LPDR* The following partial DNA sequence was identified in N. meningitidis<SEQ ID 53>: a008.seq 1 ATGAACAACA GACATTTTGC CGTCATCGCC CTGGGCAGTA ATCTTGAAAA 51 CCCTGCCCAA CAGGTACGCG CCGCATTGGA CACGCTGTCG TCCCATCCTG 101 ACATCCGTCT TAAACAGGCT TCCTCACTGT ATATGACCGC GCCCGTCGGT 151 TACGACAATC AGCCCGATTT CGTCAATGCC GTCTGCACCG TTTCCACCAC 201 CTTGGACGGC ATTGCCCTGC TTGCCGAACT CAACCGTATC GAAGCCGATT 251 TCGGACGCGA ACGCAGCTTC CGCAACGCGC CGCGCACATT GGATTTGGAC 301 ATTATCGACT TTGACGGCAT CTCCAGCGAC GACCCCCGAC TCACCCTGCC 351 GCATCCGCGC GCGCACGAAC GCAGTTTCGT CATACGCCCT TTGGCAGAAA 401 TCCTCCCTGA TTTTATTTTG GGAAAACACG GAAAGGTTGC CGAATTGTCA 451 AAACGGCTGG GCAATCAAGG CATCCGTCTT TTACCGGATA AGTAA This corresponds to the amino acid sequence <SEQ ID 54; ORF 008. a>: a008.pep 1 MΝΝRHFAVIA LGS LEΝPAQ QVRAALDTLS SHPDIRLKQA SSLYMTAPVG 51 YDΝQPDFVΝA VCTVSTTLDG IALLAELNRI EADFGRERSF RNAPRTLDLD 101 IIDFDGISSD DPRLTLPHPR AHERSFVIRP LAEILPDFIL GKHGKVAELS 151 KRLGNQGIRL LPDK* m008/a008 97.6%) identity over a 164 aa overlap 10 20 30 40 50 60 m008.pep MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLKQASSLYMTAPVGYDNQPDFVNA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a008 MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLKQASSLYMTAPVGYDNQPDFVNA 10 20 30 40 50 60 70 80 90 100 110 120 m008.pep VCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLXLDIIDFDGISSDDTRLTLPHPR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a008 VCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDIIDFDGISSDDPRLTLPHPR 70 80 90 100 110 120 130 140 150 160 m008.pep AHERSFVIRPLAEILPDFVLGKHGKVAELSKRLGNQGIRLLPDRX I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I II I I I I I I I I I : I a008 AHERSFVIRPLAEILPDFILGKHGKVAELSKRLGNQGIRLLPDKX 130 140 150 160 Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 008 shows 92.7% identity over a 164 aa overlap with a predicted ORF (ORF008.ng) from N. gonorrhoeae: m008/g008 10 20 30 40 50 60 m008.pep MΝΝRHFAVIALGSΝLEΝPAQQVRAALDTLSSHPDIRLKQASSLYMTAPVGYDΝQPDFVΝA I I I I I I I I I I I I I I I : I I I II : I : I I I: I I I I I I I I I : I: I I I I I I I I I I I I I I I I I : I I g008 MΝΝRHFAVIALGSΝLDΝPAQQIRGALDALSSHPDIRLEQVSSLYMTAPVGYDΝQPDFIΝA 10 20 30 40 50 60 70 80 90 100 110 120 m008. pep VCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLXLDIIDFDGISSDDTRLTLPHPR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g008 VCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDIIDFDGISSDDPRLTLPHPR 70 80 90 100 110 120 130 140 150 160 m008. pep AHERSFVIRPLAEILPDFVLGKHGKVAELSKRLGNQGIRLLPDRX I I I I I I I I I I I I I I I I I I : I I I: I I I : I I I I I I I I I I I I I I I I I I g008 AHERSFVIRPLAEILPDFILGKYGKVVELSKRLGNQGIRLLPDRX 130 140 150 160 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 55>: g009.seq 1 ATGCCCCGCG CTGCCGTAGC CTTTGAGCGT CATCATCACA AAAGCAAAGC 51 CGAACAAAAT ACCCATCGCC GCGCCGACGC AGAGATAGCC GAAGGCTTCG 101 CGGTTGGAAA TCAGCACACG CAGGCGCGAA ACCAGTCCGT AATGGCGGTA 151 CAGCTGCCGC TCGTCGCCTT TTCGGATAAA GTGGTTGTcg cGTTCCAAGC 201 TGTTGTTCAG GCGGAAATAC AGGTTTTCGC TGATGGCGGC AAAACGTGGC 251 AaaaGCCATA A This corresponds to the amino acid sequence <SEQ ID 56; ORF 009.ng>: g009.pep 1 MPRAAVAFER HHHKSKAEQΝ THRRADAEIA EGFAVGΝQHT QARΝQSVMAV 51 QLPLVAFSDK VWAFQAVVQ AEIQVFADGG KTWQKP* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 57>: 009.seq 1 ATGCCCCGCG CTGCTGTAGC CTTTGAGCGT CATCATCACA AAAGCAAAGC 51 CGAACAAAAT ACCCATCGCC GTGCCGACGC AGAGATAGCC GAAGGCTTCG 101 CGGTTGGAAA TCAGCACACG CAGGCGCGCA AGCAGTCCGT AATGGCGGTA 151 CAGCTGCCGC CGGTCGCCTT TTCGGATAAA GTGGTTGTCG CGTTCCAAGC 201 TGTTGTTCAG GCGGAAATAC AGGTTTTCGC TGATGGCGGC AAAACGTGGC 251 AAAAGCCATA A This corresponds to the amino acid sequence <SEQ ID 58; ORF 009>: m009.pep 1 MPRAAVAFER HHHKSKAEQΝ THRRADAEIA EGFAVGΝQHT QARKQSVMAV 51 QLPPVAFSDK VWAFQAVVQ AEIQVFADGG KTWQKP* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 009 shows 97.7% identity over a 86 aa overlap with a predicted ORF (ORF 009.ng) from N gonorrhoeae: m009/g009 10 20 30 40 50 60 mOO 9. pep MPRAAVAFERHHHKSKAEQΝTHRRADAEIAEGFAVGΝQHTQARKQSVMAVQLPPVAFSDK I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I g009 MPRAAVAFERHHHKSKAEQΝTHRRADAEIAEGFAVGΝQHTQARΝQSVMAVQLPLVAFSDK 10 20 30 40 50 60 70 80 m009. pep VVVAFQAVVQAEIQVFADGGKTWQKPX I I I I I I I I I I I I I I I I I I I I I II I I I I g009 VVVAFQAVVQAEIQVFADGGKTWQKPX 70 80 The following partial DNA sequence was identified in N meningitidis <SEQ ID 59>: a009.seq 1 ATGCCCCGCG CTGCTGTAGC CTTTGAGCGT CATCATCACA AAAGCAAAGC 51 CGAACAAAAT ACCCATCGCC GTGCCGACGC AGAGATAGCC GAAGGCTTCG 101 CGGTTGGAAA TCAGCACACG CAGGCGCGCA AGCAGTCCGT AATGGCGGTC 151 CAGCTGCCGC TCGTCGCCTT TTCGGATAAA GTGGTTGTCG CGTTCCAAGC 201 TGTTCTTCAG GCGGAAATAC AGGTTTTCGC TGATGGCGGC AAAACGTGGC 251 AAAAGCCATA A This corresponds to the amino acid sequence <SEQ ID 60; ORF 009. a>: a009.pep 1 MPRAAVAFER HHHKSKAEQΝ THRRADAEIA EGFAVGΝQHT QARKQSVMAV 51 QLPLVAFSDK VWAFQAVLQ AEIQVFADGG KTWQKP* m009/a009 91.1% identity over a 86 aa overlap 10 20 30 40 50 60 m009.pep MPRAAVAFERHHHKSKAEQΝTHRRADAEIAEGFAVGΝQHTQARKQSVMAVQLPPVAFSDK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I II I a009 MPRAAVAFERHHHKSKAEQΝTHRRADAEIAEGFAVGΝQHTQARKQSVMAVQLPLVAFSDK 10 20 30 40 50 60 70 80 m009.pep VVVAFQAVVQAEIQVFADGGKTWQKPX I I I I I I I I : I I I I I I I I I I I I I I I I I I a009 VVVAFQAVLQAEIQVFADGGKTWQKPX 70 80 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 61>: g010. seq 1 ATGGGTTTTC CTGTTCGCAA GTTTGATGCC GTGATTGTCG GCGGTGGCGG 51 TGCAGGTTTA CGTGCAGCCC TCCAATTATC CAAATCCGGT TTGAATTGTG 101 CCGTTTTGTC TAAAGTGTTC CCGACCCGCT CGCATACCGT AGCGGCGCAG 151 GGCGGTATTT CCGCCTCTCT GGGTAATGTG CAGGAGGACC GTTGGGACTG 201 GCACATGTAC GATACCGTGA AAGGTTCCGA CTGGCTGGGC GACCAAGATG 251 CGATTGAGTT TATGTGTCGC GCTGCGCCTG AAGCGGTGAT TGAGTTGGAA 301 CACATGGGTA TGCCTTTTGA CCGCGTTGAA AGCGGCAAAA TTTATCAGCG 351 TCCTTTCGGC GGACATACTG CCGAACATGG TAAACGTGCG GTAGAACGTG 401 CATGTGCGGT TGCCGACCGT ACCGGTCATG CGATGTTGCA TACTTTGTAC 451 CAACAAAACG TCCGTGCCAA TACACAATTC TTTGTGGAAT GGACGGCGCA 501 AGATTTGATT CGTGATGAAA ACGGCGATGT CGTCGGCGTA ACCGCCATGG 551 AAATGGAAAC GGGCGAAGTT TATATTTTCC ACGCCAAGGC CGTGATGTTT 601 GCTACCGGTG GCGGCGGTCG TATTTATGCT TCTTCTACCA ATGCTTATAT 651 GAATACCGGT GACGGTTTGG GCATTTGCGC CCGTGCGGGC ATTCCGTTGG 701 AAGATATGGA ATTCTGGCAA TTCCACCCGA CCGGCGTGGC GGGTGCGGGC 751 GTGTTGATTA CCGAAGGCGT ACGCGGCGAG GGCGGTATTC TGTTGAacgc 801 cgacggcgaA cgcTTTATGG AAcgctatgc GCcgACCGta aAagaCTTGG 851 CTTCTCGCga cgtGGTTTCA CgcgcGatgG CGatggaAAt ctatgaaggt 901 cgcggctgTG GtaaAAAcaA agaCCacgtC TTACTGAAAA TCGACcAtAt 951 cggtGCAGAA AAAATTATGG AAAAACTGCC GGGCATCCGC GAGATTTCCA 1001 TTCagtttgc cGGTATCGAT CCGATTAAAG ACCCGATTcc ggttgTGCCG 1051 ACTACCCACT ATATGATGGG CGGCATTCcg aCCAATTATC ACGGTGAAGT 1101 TGTTGTTCCG CAAGGCGACG AGTACGAAGT ACCTGTAAAA GGCCTGTATG 1151 CCGCAGGTGA GTGCGCCTGT GCTTCCGTAC ACGGTGCGAA CCGTTTGGGT 1201 ACGAACTCCC TGCTGGACTT GGTGGTGTTC cgcccaaccc cccggtga This corresponds to the amino acid sequence <SEQ ID 62; ORF 010.ng>: gOlO .pep 1 MGFPVRKFDA VIVGGGGAGL RAALQLSKSG LNCAVLSKVF PTRSHTVAAQ 51 GGISASLGNV QEDRWDWHMY DTVKGSDWLG DQDAIEFMCR AAPEAVIELE 101 HMGMPFDRVE SGKIYQRPFG GHTAEHGKRA VERACAVADR TGHAMLHTLY 151 QQNVRANTQF FVEWTAQDLI RDENGDVVGV TAMEMETGEV YIFHAKAVMF 201 ATGGGGRIYA SSTNAYMNTG DGLGICARAG IPLEDMEFWQ FHPTGVAGAG 251 VLITEGVRGE GGILLNADGE RFMERYAPTV KDLASRDVVS RAMAMEIYEG 301 RGCGKNKDHV LLKIDHIGAE KIMEKLPGIR EISIQFAGID PIKDPIPWP 351 TTHYMMGGIP TNYHGEWVP QGDEYEVPVK GLYAAGECAC ASVHGANRLG 401 TNSLLDLWF RPTPR* The following partial DNA sequence was identified in N meningitidis <SEQ ID 63>: mOlO.seq (PARTIAL) 1 .. nTCCAATTAT CCAAATCCGG TCTGAATTGT GCCGTTTTGT CTAAAGTGTT 51 CCCGACCCGT TCGCATACCG TAGCGGCGCA GGGCGGTATT TCCGCCTCTn 101 TGGGTAATGT GCAGGAAGAC CGTTGGGACT GGCACATGTA CGATACCGTG 151 AAAGGTTCCG ACTGGTTGGG CGACCAAGAT GCGATTGAGT TTATGTGCCG 201 CGCCGCGCCT GAAGCCGTAA TTGAGTTGGA ACACATGGGT ATGCCTTTTG 251 ACCGTGTGGA AAGCGGTAAA ATTTATCAGC GTCCTTTCGG CGGCCATACT 301 GCCGAACACG GTAAACGCGC GGTAGAACGC GyCTGTGCGG TTGCCGACCG 351 TACAGGTCAT GCGATGCTGC ATACTTTGTA CCAACAAAAC GTCCGTGCCA 401 ATACGCAATT CTTTGTGGAA TGGACGGCAC AAGATTTGAT TCGTGATGAA 451 AACGGCGATG TCGTCGGCGT AACCGCCATG GAAATGGAAA CCGGCGAAgT 501 TTATATTTTC CACGCTAAAG CTGTGATGTT TGCTACCGGC GGCGGCGGTC 551 GTATTTATGC GTCTTCTACC AATGCCTATA TGAATACCGG CGATGGTTTG 601 GGTATTTGTG CGCGTGCAGG TATCCCGTTG GAAGACATGG AATTCTGGCA 651 ATTCCAGCCG ACCGGCGTGG CGGGTGCGGG CGTGTTGATT ACCGAA.... This corresponds to the amino acid sequence <SEQ ID 64; ORF 010>: mOlO.pep (PARTIAL) 1 .. XQLSKSGLΝC AVLSKVFPTR SHTVAAQGGI SASXGΝVQED RWDWHMYDTV 51 KGSDWLGDQD AIEFMCRAAP EAVIELEHMG MPFDRVESGK IYQRPFGGHT 101 AEHGKRAVER XCAVADRTGH AMLHTLYQQΝ VRAΝTQFFVE WTAQDLIRDE 151 ΝGDVVGVTAM EMETGEVYIF HAKAVMFATG GGGRIYASST ΝAYMΝTGDGL 201 GICARAGIPL EDMEFWQFQP TGVAGAGVLI TE ... The following partial DΝA sequence was identified in N meningitidis <SEQ ID 65>: aOlO.seq 1 ATGGGCTTTC CTGTTCGCAA GTTTGATGCC GTGATTGTCG GCGGTGGTGG 51 TGCAGGTTTA CGCGCAΝCCC TCCAATTATC CAAATCCGGT CTGAATTGTG 101 CCGTTTTGTC TAAAGTGTTC CCGACCCGTT CGCATACCGT AGCGGCGCAG 151 GGCGGTATTT CCGCCTCTCT GGGTAATGTG CAGGAAGACC GTTGGGACTG 201 GCACATGTAC GATACCGTGA AAGGTTCCGA CTGGTTGGGC GACCAAGATG 251 CGATTGAGTT TATGTGCCGC GCCGCGCCTG AAGCCGTAAT TGAGTTGGAA 301 CACATGGGTA TGCCTTTTGA CCGTGTGGAA AGCGGTAAAA TTTATCAGCG 351 TCCTTTCGGC GGCCATACTG CCGAACACGG TAAACGCGCG GTAGAACGCG 401 CCTGTGCΝGT TGCCGACCGT ACAGGTCATG CGATGCTGCA TACTTTGTAC 451 CAACAAAATG TCCGTGCCAA TACGCAATTC TTTGTGGAAT GGACGGCACA 501 AGATTTGATT CGTGATGAAA ACGGCGATGT CGTCGGCGTA ACCGCCATGG 551 AAATGGAAAC CGGCGAAGTT TATATTTTCC ACGCTAAAGC TGTGATGTTT 601 GCTACCGGCG GCGGCGGCCG TATTTATGCG TCTTCTACCA ATGCCTATAT 651 GAATACCGGC GATGGTTTGG GTATTTGTGC GCGTGCAGGT ATCCCGTTGG 701 AAGACATGGA ATTCTGGCAA TTCCACCCGA CCGGCGTGGC AGGTGCGGGC 751 GTGTTGATTA CCGAAGGCGT ACGCGGCGAG GGCGGTATTC TGTTGAATGC 801 CGACGGCGAA CGCTTTATGG AACGCTATGC GCCGACCGTA AAAGACTTGG 851 CTTCTCGCGA CGTTGTTTCC CGCGCGATGG CGATGGAAAT CTACGAAGGT 901 CGCGGCTGCG GTAAAAACAA AGACCATGTC TTACTGAAAA TCGACCATAT 951 CGGCGCAGAA AAAATTATGG AAAAACTGCC GGGCATCCGC GAGATTTCCA 1001 TTCAGTTCGC CGGTATCGAT CCGATTAAAG ACCCGATTCC CGTTGTGCCG 1051 ACTACCCACT ATATGATGGG CGGTATTCCG ACCAACTACC ATGGCGAAGT 1101 TGTCGTTCCT CAAGGCGACG AATACGAAGT GCCTGTAAAA GGTCTGTATG 1151 CGGCAGGTGA GTGCGCCTGT GCTTCCGTAC ACGGTGCGAA CCGCTTGGGT 1201 ACGAACTCCC TGCTGGACTT AGTGGTATTC GGTAAAGCTG CCGGCGACAG 1251 CATGATTAAA TTCATCAAAG AGCAAAGCGA CTGGAAACCT TTGCCTGCTA 1301 ATGCCGGCGA ACTGACCCGC CAACGTATCG AGCGTTTGGA CAATCAAACT 1351 GATGGTGAAA ACGTTGATGC ATTGCGCCGC GAACTGCAAC GCTCCGTACA 1401 ATTGCACGCC GGCGTGTTCC GTACTGATGA GATTCTGAGC AAAGGCGTTC 1451 GAGAAGTCAT GGCGATTGCC GAGCGTGTGA AACGTACCGA AATCAAAGAC 1501 AAGAGCAAAG TGTGGAATAC CGCGCGTATC GAGGCTTTGG AATTGGATAA 1551 CCTAATTGAA GTGGCGAAAG CGACTTTGGT GTCTGCCGAA GCACGTAAAG 1601 AATCACGCGG TGCGCACGCT TCAGACGACC ATCCTGAGCG CGATGATGAA 1651 AACTGGATGA AACATACGCT GTACCATTCA GATGCCAATA CCTTGTCCTA 1701 CAAACCGGTG CACACCAAGC CTTTGAGCGT GGAATACATC AAACCGGCCA 1751 AGCGCGTTTA TTGA This corresponds to the amino acid sequence <SEQ ID 66; ORF 010.a>: a010. pep 1 MGFPVRKFDA VIVGGGGAGL RAXLQLSKSG LNCAVLSKVF PTRSHTVAAQ 51 GGISASLGNV QEDRWDWHMY DTVKGSDWLG DQDAIEFMCR AAPEAVIELE 101 HMGMPFDRVE SGKIYQRPFG GHTAEHGKRA VERACAVADR TGHAMLHTLY 151 QQNVRANTQF FVEWTAQDLI RDENGDVVGV TAMEMETGEV YIFHAKAVMF 201 ATGGGGRIYA SSTNAYMNTG DGLGICARAG IPLEDMEFWQ FHPTGVAGAG 251 VLITEGVRGE GGILLNADGE RFMERYAPTV KDLASRDVVS RAMAMEIYEG 301 RGCGKNKDHV LLKIDHIGAE KIMEKLPGIR EISIQFAGID PIKDPIPWP 351 TTHYMMGGIP TNYHGEWVP QGDEYEVPVK GLYAAGECAC ASVHGANRLG 401 TNSLLDLWF GKAAGDSMIK FIKEQSDWKP LPANAGELTR QRIERLDNQT 451 DGENVDALRR ELQRSVQLHA GVFRTDEILS KGVREVMAIA ERVKRTEIKD 501 KSKVWNTARI EALELDNLIE VAKATLVSAE ARKESRGAHA SDDHPERDDE 551 NWMKHTLYHS DANTLSYKPV HTKPLSVEYI KPAKRVY* moio/aoio 98.7%> identity over a 231 aa overlap 10 20 30 mOlO .pep XQLSKSGLNCAVLSKVFPTRSHTVAAQGGISASXGNV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I III aOlO MGFPVRKFDAVIVGGGGAGLRAXLQLSKSGLNCAVLSKVFPTRSHTVAAQGGISASLGNV 10 20 30 40 50 60 40 50 60 70 80 90 mOlO.pep QEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHMGMPFDRVESGKIYQRPFG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I aOlO QEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHMGMPFDRVESGKIYQRPFG 70 80 90 100 110 120 100 110 120 130 140 150 mOl0. pep GHTAEHGKRAVERXCAVADRTGHAMLHTLYQQNVRANTQFFVEWTAQDLIRDENGDWGV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I aOlO GHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQFFVEWTAQDLIRDENGDWGV 130 140 150 160 170 180 160 170 180 190 200 210 OlO . pep TAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMNTGDGLGICARAGIPLEDMEFWQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I aOlO TAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMNTGDGLGICARAGIPLEDMEFWQ 190 200 210 220 230 240 220 230 mOlO.pep FQPTGVAGAGVLITE I : I I I I I I I I I I I I I aOlO FHPTGVAGAGVLITEGVRGEGGILLNADGERFMERYAPTVKDLASRDVVSRAMAMEIYEG 250 260 270 280 290 300 Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 010 shows 98.7% identity over a 231 aa overlap with a predicted ORF (ORF OlO.ng) from N. gonorrhoeae: mOlO.pep/gOlO.pep 10 20 30 mOlO . pep XQLSKSGLΝCAVLSKVFPTRSHTVAAQGGISASXGΝV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I III gOlO MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLΝCAVLSKVFPTRSHTVAAQGGISASLGΝV 10 20 30 40 50 60 40 50 60 70 80 90 mOlO.pep QEDRWDWHMYDTVKGΞDWLGDQDAIEFMCRAAPEAVIELEHMGMPFDRVESGKIYQRPFG I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I gOlO QEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHMGMPFDRVESGKIYQRPFG 70 80 90 100 110 120 100 110 120 130 140 150 mOlO . pep GHTAEHGKRAVERXCAVADRTGHAMLHTLYQQΝVRAΝTQFFVEWTAQDLIRDEΝGDVVGV I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gOlO GHTAEHGKRAVERACAVADRTGHAMLHTLYQQΝVRAΝTQFFVEWTAQDLIRDEΝGDWGV 130 140 150 160 170 180 160 170 180 190 200 210 mOlO . pep TAMEMETGEVYIFHAKAVMFATGGGGRIYASSTΝAYMΝTGDGLGICARAGIPLEDMEFWQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I gOlO TAMEMETGEVYIFHAKAVMFATGGGGRIYASSTΝAYMΝTGDGLGICARAGIPLEDMEFWQ 190 200 210 220 230 240 220 230 mOlO.pep FQPTGVAGAGVLITE I : I I I I I I I I I I I I I gOlO FHPTGVAGAGVLITEGVRGEGGILLΝADGERFMERYAPTVKDLASRDWSRAMAMEIYEG 250 260 270 280 290 300 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 67>: g010-l.seq.. 1 ATGGGTTTTC CTGTTCGCAA GTTTGATGCC GTGATTGTCG GCGGTGGCGG 51 TGCAGGTTTA CGTGCAGCCC TCCAATTATC CAAATCCGGT TTGAATTGTG 101 CCGTTTTGTC TAAAGTGTTC CCGACCCGCT CGCATACCGT AGCGGCGCAG 151 GGCGGTATTT CCGCCTCTCT GGGTAATGTG CAGGAGGACC GTTGGGACTG 201 GCACATGTAC GATACCGTGA AAGGTTCCGA CTGGCTGGGC GACCAAGATG 251 CGATTGAGTT TATGTGTCGC GCTGCGCCTG AAGCGGTGAT TGAGTTGGAA 301 CACATGGGTA TGCCTTTTGA CCGCGTTGAA AGCGGCAAAA TTTATCAGCG 351 TCCTTTCGGC GGACATACTG CCGAACATGG TAAACGTGCG GTAGAACGTG 401 CATGTGCGGT TGCCGACCGT ACCGGTCATG CGATGTTGCA TACTTTGTAC 451 CAACAAAACG TCCGTGCCAA TACACAATTC TTTGTGGAAT GGACGGCGCA 501 AGATTTGATT CGTGATGAAA ACGGCGATGT CGTCGGCGTA ACCGCCATGG 551 AAATGGAAAC GGGCGAAGTT TATATTTTCC ACGCCAAGGC CGTGATGTTT 601 GCTACCGGTG GCGGCGGTCG TATTTATGCT TCTTCTACCA ATGCTTATAT 651 GAATACCGGT GACGGTTTGG GCATTTGCGC CCGTGCGGGC ATTCCGTTGG 701 AAGATATGGA ATTCTGGCAA TTCCACCCGA CCGGCGTGGC GGGTGCGGGC 751 GTGTTGATTA CCGAAGGCGT ACGCGGCGAG GGCGGTATTC TGTTGAACGC 801 CGACGGCGAA CGCTTTATGG AACGCTATGC GCCGACCGTA AAAGACTTGG 851 CTTCTCGCGA CGTGGTTTCA CGCGCGATGG CGATGGAAAT CTATGAAGGT 901 CGCGGCTGTG GTAAAAACAA AGACCACGTC TTACTGAAAA TCGACCATAT 951 CGGTGCAGAA AAAATTATGG AAAAACTGCC GGGCATCCGC GAGATTTCCA 1001 TTCAGTTTGC CGGTATCGAT CCGATTAAAG ACCCGATTCC GGTTGTGCCG 1051 ACTACCCACT ATATGATGGG CGGCATTCCG ACCAATTATC ACGGTGAAGT 1101 TGTTGTTCCG CAAGGCGACG AGTACGAAGT ACCTGTAAAA GGCCTGTATG 1151 CCGCAGGTGA GTGCGCCTGT GCTTCCGTAC ACGGTGCGAA CCGTTTGGGT 1201 ACGAACTCCC TGCTGGACTT GGTGGTGTTC cgcccaaccc cccggtga This corresponds to the amino acid sequence <SEQ ID 68; ORF 010-1. ng>: g010-l.pep 1 MGFPVRKFDA VIVGGGGAGL RAALQLSKSG LNCAVLSKVF PTRSHTVAAQ 51 GGISASLGNV QEDRWDWHMY DTVKGSDWLG DQDAIEFMCR AAPEAVIELE 101 HMGMPFDRVE SGKIYQRPFG GHTAEHGKRA VERACAVADR TGHAMLHTLY 151 QQNVRANTQF FVEWTAQDLI RDENGDVVGV TAMEMETGEV YIFHAKAVMF 201 ATGGGGRIYA SSTNAYMNTG DGLGICARAG IPLEDMEFWQ FHPTGVAGAG 251 VLITEGVRGE GGILLNADGE RFMERYAPTV KDLASRDVVS RAMAMEIYEG 301 RGCGKNKDHV LLKIDHIGAE KIMEKLPGIR EISIQFAGID PIKDPIPWP 351 TTHYMMGGIP TNYHGEWVP QGDEYEVPVK GLYAAGECAC ASVHGANRLG 401 TNSLLDLWF RPTPR* gOlO-1 / P10444 sp|P10444|DHSA_ECOLI SUCCINATE DEHYDROGENASE FLAVOPROTEIN SUBUNIT gnl|PID|dlO15210 (D90711) Succinate dehydrogenase, flavoprotein [Escherichia coli] gi|l786942 (AE000175) succinate dehydrogenase flavoprotein subunit [Escherichia coli] Length = 588 Score = 1073 (495.6 bits) , Expect = 6.7e-169, Sum P(2) = 6.7e-169 Identities = 191/303 (63%) , Positives = 238/303 (78%) Query : 1 MGFPVRKFDAVIVXXXXXXXXXXXXXSKSGLNCAVLSKVFPTRSHTVAAQGGI SASLGNV 60 M PVR+FDAV++ S+SG CA+LSKVFPTRΞHTV+AQGGI+ +LGN Sb j ct : 1 KLPVREFDAWIGAGGAGMRAALQISQSGQTCALLSKVFPTRSHTVSAQGGITVALGNT 60 Query: 61 QEDR D HMYDTVKGSD LGDQDAIEF CRAAPEAVIELEHMG PFDRVESGKIYQRPFG 120 ED W+WHMYDTVKGSD++GDQDAIE+MC+ PEA++ELEHMG+PF R++ G+IYQRPFG Sbjct: 61 HEDN E HMYDTVKGΞDYIGDQDAIEYMCKTGPEAILELEHMGLPFSRLDDGRIYQRPFG 120 Query: 121 GHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQFFVE TAQDLIRDENGDWGV 180 G + G R A ADRTGHA+LHTLYQQN++ +T F E A DL++ +++G WG Sbjct: 121 GQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTIFSE YALDLVKNQDGAWGC 180 Query: 181 TAME ETGEVYIFHAKAVMFATGGGGRIYASSTNAY NTGDGLGICARAGIPLEDMEFWQ 240 TA+ +ETGEV F A+A + ATGG GRIY S+TNA++NTGDG+G+ RAG+P++DME Q Sbjct: 181 TALCIETGEWYFKARATVLATGGAGRI YQSTTNAHINTGDGVGMAIRAGVPVQD EMWQ 240 Query: 241 FHPTGVAGAGVLITEGVRGEGGILLNADGERF ERYAPTVKDLAΞRDWSRAMAMEIYEG 300 FHPTG+AGAGVL+TEG RGEGG LLN GERF ERYAP KDLA RDW+R++ +EI EG Sbjct: 241 FHPTGIAGAGVLVTEGCRGEGGYLLNKHGERF ERYAPNAKDLAGRDWARSIMIEIREG 300 Query: 301 RGC 303 RGC Sbjct: 301 RGC 303 Score = 249 (115.0 bits) , Expect = 6.7e-169, Sura P(2) = 6.7e-169 Identities = 53/102 (51%) , Positives = 62/102 (60%) Query: 309 HVLLKIDHIGAEKIMEKLPGIREISIQFAGXXXXXXXXXXXXTTHYMMGGIPTNYHGEW 368 H K+DH+G E + +LPGI E+S FA T HYMMGGIPT G+ + Sbjct: 310 HAKLKLDHIiGKEVLESRLPGILE SRTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQAL 369 Query: 369 VPQGDEYEVPVKGLYAAGECACASVHGANRLGTNSLLDLWF 410 +V V GL+A GE AC SVHGANRLG NSLLDLWF Sbjct: 370 TVNEKGEDVWPGLFAVGEIACVSVHGANR GGNSLLDLWF 411 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 69>: OlO-l.seq.. 1 ATGGGTTTTC CTGTTCGCAA GTTTGATGCC GTGATTGTCG GCGGTGGTGG 51 TGCAGGTTTA CGCGCAGCCC TCCAATTATC CAAATCCGGT CTGAATTGTG 101 CCGTTTTGTC TAAAGTGTTC CCGACCCGTT CGCATACCGT AGCGGCGCAg 151 GGCGGTATTT CCGCCTCTCT GGGTAATGTG CAGGAAGACC GTTGGGACTG 201 GCACATGTAC GATACCGTGA AAGGTTCCGA CTGGTTGGGC GACCAAGATG 251 CGATTGAGTT TATGTGCCGC GCCGCGCCTG AAGCCGTAAT TGAGTTGGAA 301 CACATGGGTA TGCCTTTTGA CCGTGTGGAA AGCGGTAAAA TTTATCAGCG 351 TCCTTTCGGC GGCCATACTG CCGAACACGG TAAACGCGCG GTAGAACGCG 401 CCTGTGCGGT TGCCGACCGT ACAGGTCATG CGATGCTGCA TACTTTGTAC 451 CAACAAAACG TCCGTGCCAA TACGCAATTC TTTGTGGAAT GGACGGCACA 501 AGATTTGATT CGTGATGAAA ACGGCGATGT CGTCGGCGTA ACCGCCATGG 551 AAATGGAAAC CGGCGAAGTT TATATTTTCC ACGCTAAAGC TGTGATGTTT 601 GCTACCGGCG GCGGCGGTCG TATTTATGCG TCTTCTACCA ATGCCTATAT 651 GAATACCGGC GATGGTTTGG GTATTTGTGC GCGTGCAGGT ATCCCGTTGG 701 AAGACATGGA ATTCTGGCAA TTCCACCCGA CCGGCGTGGC GGGTGCGGGC 751 GTGTTGATTA CCGAAGGCGT ACGCGGCGAG GGCGGTATTC TGTTGAATGC 801 CGACGGCGAA CGCTTTATGG AACGCTATGC GCCGACCGTA AAAGACTTGG 851 CTTCTCGCGA CGTTGTTTCC CGCGCGATGG CGATGGAAAT CTACGAAGGT 901 CGCGGCTGCG GTAAAAACAA AGACCATGTC TTACTGAAAA TCGACCATAT 951 CGGCGCAGAA AAAATTATGG AAAAACTGCC GGGCATCCGC GAGATTTCCA 1001 TTCAGTTCGC CGGTATCGAT CCGATTAAAG ACCCGATTCC CGTTGTGCCG 1051 ACTACCCACT ATATGATGGG CGGCATTCCG ACCAATTACC ACGGCGAAGT 1101 TGTCGTTCCG CAAGGTGAAG ATTACGAAGT GCCTGTAAAA GGTCTGTATG 1151 CGGCAGGTGA GTGCGCTTGT GCTTCCGTAC ACGGTGCGAA CCGCTTGGGT 1201 ACCAACTCCC TGTTGGACTT GGTGGTATTC GGTAAAGCTG CCGGCGACAG 1251 CATGATTAAA TTCATCAAAG AGCAAAGCGA CTGGAAACCT TTGCCTGCTA 1301 ATGCAGGTGA GTTGACCCGC CAACGTATCG AGCGTTTGGA CAACCAAACC 1351 GATGGTGAAA ACGTTGATGC ATTGCGTCGC GAACTGCAAC GCTCTGTACA 1401 ACTGCACGCC GGCGTGTTCC GTACTGATGA GATTCTGAGC AAAGGCGTTC 1451 GAGAAGTCAT GGCGATTGCC GAGCGTGTGA AACGTACCGA AATCAAAGAC 1501 AAGAGCAAAG TGTGGAATAC CGCGCGTATC GAGGCTTTGG AATTGGATAA 1551 CCTGATTGAA GTGGCGAAAG CGACTTTGGT GTCTGCCGAA GCACGTAAAG 1601 AATCACGCGG TGCGCACGCT TCAGACGACC ATCCTGAGCG CGATGATGAA 1651 AACTGGATGA AACATACGCT GTACCATTCA GATATCAATA CCTTGTCCTA 1701 CAAACCGGTG CACACCAAGC CTTTGAGCGT GGAATACATC AAACCGGCCA 1751 AGCGCGTTTA TTGATGA This corresponds to the amino acid sequence <SEQ ID 70; ORF 010-1>: mOlO-l.pep.. 1 MGFPVRKFDA VIVGGGGAGL RAALQLSKSG LNCAVLSKVF PTRSHTVAAQ 51 GGISASLGNV QEDRWDWHMY DTVKGSDWLG DQDAIEFMCR AAPEAVIELE 101 HMGMPFDRVE SGKIYQRPFG GHTAEHGKRA VERACAVADR TGHAMLHTLY 151 QQNVRANTQF FVEWTAQDLI RDENGDVVGV TAMEMETGEV YIFHAKAVMF 201 ATGGGGRIYA SSTNAYMNTG DGLGICARAG IPLEDMEFWQ FHPTGVAGAG 251 VLITEGVRGE GGILLNADGE RFMERYAPTV KDLASRDVVS RAMAMEIYEG 301 RGCGKNKDHV LLKIDHIGAE KIMEKLPGIR EISIQFAGID PIKDPIPWP 351 TTHYMMGGIP TNYHGEWVP QGEDYEVPVK GLYAAGECAC ASVHGANRLG 401 TNSLLDLWF GKAAGDSMIK FIKEQSDWKP LPANAGELTR QRIERLDNQT 451 DGENVDALRR ELQRSVQLHA GVFRTDEILS KGVREVMAIA ERVKRTEIKD 501 KSKVWNTARI EALELDNLIE VAKATLVSAE ARKESRGAHA SDDHPERDDE 551 NWMKHTLYHS DINTLSYKPV HTKPLSVEYI KPAKRVY* mOlO-1 / gOlO-1 99.5% identity in 410 aa overlap 10 20 30 40 50 60 mOlO-1.pep MGPPVIUaOAVIVGGGGAG RAALQLSKSGLNCAVLSKVFPTRSHTVAAQGGISASLGNV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II gOlO-1 MGFPVKKSDAVIVGGGGAGLRAALQLSKSG NCAVLSKV PTRSHTVAAQGGISASLGNV 10 20 30 40 50 60 70 80 90 100 110 120 OlO-l .pep QEDRWDWHMΪDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHMGMPFDRVESGKIYQRPFG I I II I III II I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I II I I I gOlO-1 QEDRMD HMYDTVKGSDMLGDQDAIEFMCRAAPEAVIELEHMGMPFDRVESGKIYQRPFG 70 80 90 100 110 120 130 140 150 160 170 180 mθio-1.pep GHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQFFVEVJTAQDLIRDENGDWGV I I I I I I I I I I I I I I I I I I I I II I II I I I I I I I I I I I I I II I I I I I I I I I I I I II I II I I I gOlO-1 GHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQFFVEWTAQDLIRDENGDWGV 130 140 150 160 170 180 190 200 210 220 230 240 mOlO-1.pep TAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMNTGDGLGICARAGIP EDMEF Q I I II III I II I I I I I I I I I I II I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I gOlO-1 TAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMNTGDGLGICARAGIP EDMEFWQ 190 200 210 220 230 240 250 260 270 280 290 300 mOlO-l .pep FHPTGVAGAGVLITEGVRGEGGILLNADGERFMERYAPTVKD ASRDWSRAMAMEIYEG I I I I I I I I II II I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I gOlO-1 FHPTGVAGAGVLITEGVRGEGGILI^ADGERFMERYAPTVKD ASRDVVSRAMAMEIYEG 250 260 270 280 290 300 310 320 330 340 350 360 mOlO-l .pep RGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISIQFAGIDPIKDPIPWPTTHYMMGGIP I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gOlO-1 RGCGKNKDHVL KIDHIGAEKIMEKLPGIREISIQFAGIDPIKDPIPWPTTHYMMGGIP 310 320 330 340 350 360 370 380 390 400 410 420 mOlO-l .pep TNYHGEVVVPQGEDYEVPVKGLYAAGECACASVHGANRLGTNSLLD VVFGKAAGDSMIK I I I I I I I I I I I I::| I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gOlO-1 TNYHGEVWPQGDEYEVPVKGLYAAGECACASVHGANRLGTNSLLDLWFRPTPRX 370 380 390 400 410 430 440 450 460 470 480 mOlO-l .pep FIKEQSD KPLPANAGELTRQRIERLDNQTDGENVDA RRELQRSVQLHAGVFRTDEI S The following partial DNA sequence was identified in N meningitidis <SEQ ID 71>: a010-l.seq.. 1 ATGGGCTTTC CTGTTCGCAA GTTTGATGCC GTGATTGTCG GCGGTGGTGG 51 TGCAGGTTTA CGCGCAΝCCC TCCAATTATC CAAATCCGGT CTGAATTGTG 101 CCGTTTTGTC TAAAGTGTTC CCGACCCGTT CGCATACCGT AGCGGCGCAG 151 GGCGGTATTT CCGCCTCTCT GGGTAATGTG CAGGAAGACC GTTGGGACTG 201 GCACATGTAC GATACCGTGA AAGGTTCCGA CTGGTTGGGC GACCAAGATG 251 CGATTGAGTT TATGTGCCGC GCCGCGCCTG AAGCCGTAAT TGAGTTGGAA 301 CACATGGGTA TGCCTTTTGA CCGTGTGGAA AGCGGTAAAA TTTATCAGCG 351 TCCTTTCGGC GGCCATACTG CCGAACACGG TAAACGCGCG GTAGAACGCG 401 CCTGTGCΝGT TGCCGACCGT ACAGGTCATG CGATGCTGCA TACTTTGTAC 451 CAACAAAATG TCCGTGCCAA TACGCAATTC TTTGTGGAAT GGACGGCACA 501 AGATTTGATT CGTGATGAAA ACGGCGATGT CGTCGGCGTA ACCGCCATGG 551 AAATGGAAAC CGGCGAAGTT TATATTTTCC ACGCTAAAGC TGTGATGTTT 601 GCTACCGGCG GCGGCGGCCG TATTTATGCG TCTTCTACCA ATGCCTATAT 651 GAATACCGGC GATGGTTTGG GTATTTGTGC GCGTGCAGGT ATCCCGTTGG 701 AAGACATGGA ATTCTGGCAA TTCCACCCGA CCGGCGTGGC AGGTGCGGGC 751 GTGTTGATTA CCGAAGGCGT ACGCGGCGAG GGCGGTATTC TGTTGAATGC 801 CGACGGCGAA CGCTTTATGG AACGCTATGC GCCGACCGTA AAAGACTTGG 851 CTTCTCGCGA CGTTGTTTCC CGCGCGATGG CGATGGAAAT CTACGAAGGT 901 CGCGGCTGCG GTAAAAACAA AGACCATGTC TTACTGAAAA TCGACCATAT 951 CGGCGCAGAA AAAATTATGG AAAAACTGCC GGGCATCCGC GAGATTTCCA 1001 TTCAGTTCGC CGGTATCGAT CCGATTAAAG ACCCGATTCC CGTTGTGCCG 1051 ACTACCCACT ATATGATGGG CGGTATTCCG ACCAACTACC ATGGCGAAGT 1101 TGTCGTTCCT CAAGGCGACG AATACGAAGT GCCTGTAAAA GGTCTGTATG 1151 CGGCAGGTGA GTGCGCCTGT GCTTCCGTAC ACGGTGCGAA CCGCTTGGGT 1201 ACGAACTCCC TGCTGGACTT AGTGGTATTC GGTAAAGCTG CCGGCGACAG 1251 CATGATTAAA TTCATCAAAG AGCAAAGCGA CTGGAAACCT TTGCCTGCTA 1301 ATGCCGGCGA ACTGACCCGC CAACGTATCG AGCGTTTGGA CAATCAAACT 1351 GATGGTGAAA ACGTTGATGC ATTGCGCCGC GAACTGCAAC GCTCCGTACA 1401 ATTGCACGCC GGCGTGTTCC GTACTGATGA GATTCTGAGC AAAGGCGTTC 1451 GAGAAGTCAT GGCGATTGCC GAGCGTGTGA AACGTACCGA AATCAAAGAC 1501 AAGAGCAAAG TGTGGAATAC CGCGCGTATC GAGGCTTTGG AATTGGATAA 1551 CCTAATTGAA GTGGCGAAAG CGACTTTGGT GTCTGCCGAA GCACGTAAAG 1601 AATCACGCGG TGCGCACGCT TCAGACGACC ATCCTGAGCG CGATGATGAA 1651 AACTGGATGA AACATACGCT GTACCATTCA GATGCCAATA CCTTGTCCTA 1701 CAAACCGGTG CACACCAAGC CTTTGAGCGT GGAATACATC AAACCGGCCA 1751 AGCGCGTTTA TTGA This corresponds to the amino acid sequence <SEQ ID 72; ORF 010-l.a>: a010-l.pep.. 1 MGFPVRKFDA VIVGGGGAGL RAXLQLSKSG LNCAVLSKVF PTRSHTVAAQ 51 GGISASLGNV QEDRWDWHMY DTVKGSDWLG DQDAIEFMCR AAPEAVIELE 101 HMGMPFDRVE SGKIYQRPFG GHTAEHGKRA VERACAVADR TGHAMLHTLY 151 QQNVRANTQF FVEWTAQDLI RDENGDVVGV TAMEMETGEV YIFHAKAVMF 201 ATGGGGRIYA SSTNAYMNTG DGLGICARAG IPLEDMEFWQ FHPTGVAGAG 251 VLITEGVRGE GGILLNADGE RFMERYAPTV KDLASRDVVS RAMAMEIYEG 301 RGCGKNKDHV LLKIDHIGAE KIMEKLPGIR EISIQFAGID PIKDPIPWP 351 TTHYMMGGIP TNYHGEWVP QGDEYEVPVK GLYAAGECAC ASVHGANRLG 401 TNSLLDLWF GKAAGDSMIK FIKEQSDWKP LPANAGELTR QRIERLDNQT 451 DGENVDALRR ELQRSVQLHA GVFRTDEILS KGVREVMAIA ERVKRTEIKD 501 KSKVWNTARI EALELDNLIE VAKATLVSAE ARKESRGAHA SDDHPERDDE 551 NWMKHTLYHS DANTLSYKPV HTKPLSVEYI KPAKRVY* mOlO-l / aOlO-l 99.3% identity in 587 aa overlap 10 20 30 40 50 60 aOlO-l.pep MGFPVRKFDAVIVGGGGAGLRAXLQLSKSGLNCAVLΞKVFPTRSHTVAAQGGIΞASLGNV I I I I M I I M I I I I I M I M I I I I I 1 1 1 1 I I 1 1 1 I I 1 1 1 I I I I I I I 1 1 1 1 1 1 1 1 1 1 1 1 1 aO10-1 MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRSHTVAAQGGISASLGNV 10 20 30 40 50 60 70 80 90 100 110 120 aOlO-l .pep QEDRWDWH YDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHMGMPFDRVESGKIYQRPFG mOlO-1 QEDRWDWH YDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHMGMPFDRVESGKIYQRPFG 70 80 90 100 110 120 130 140 150 160 170 180 aOlO-l .pep GHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQFFVEWTAQDLIRDENGDWGV mO10-1 GHTAEHGKRAVERACAVADRTGHA LHTLYQQNVRANTQFFVE TAQDLIRDENGDWGV 130 140 150 160 170 180 190 200 210 220 230 240 aOlO-l .pep TAME ETGEVYIFHAKAVMFATGGGGRIYASSTNAYMNTGDGLGICARAGIPLEDMEFWQ mOlO-1 TAME ETGEVYIFHAKAVMFATGGGGRIYASSTNAYMNTGDGLGICARAGIPLEDMEF Q 190 200 210 220 230 240 250 260 270 280 290 300 aOlO-l .pep FHPTGVAGAGVLITEGVRGEGGILLNADGERFMERYAPTVKDLASRDWSRAMAMEIYEG mOlO-1 FHPTGVAGAGVLITEGVRGEGGILLNADGERF ERYAPTVKDLASRDWSRAMAMEIYEG 250 260 270 280 290 300 310 320 330 340 350 360 aOlO-l.pep RGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISIQFAGIDPIKDPIPWPTTHYMMGGIP mOlO-1 RGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISIQFAGIDPIKDPIPWPTTHYMMGGIP 310 320 330 340 350 360 370 380 390 400 410 420 aOlO -l .pep TNYHGEWVPQGDEYEVPVKGLYAAGECACASVHGANRLGTNSLLDLWFGKAAGDSMIK mO 10 - 1 TNYHGEVWPQGEDYEVP VKGLYAAGECACAS VHGANRLG NSLLDLWFGKAAGDSMI 370 380 390 400 410 420 430 440 450 460 470 480 aOlO-l .pep FIKEQSD KPLPANAGELTRQRIERLDNQTDGENVDALRRELQRSVQLHAGVFRTDEILS mOlO-1 FIKEQSD KPLPANAGELTRQRIERLDNQTDGENVDALRRELQRSVQLHAGVFRTDEILΞ 430 440 450 460 470 480 490 500 510 520 530 540 aOlO-l .pep KGVREVMAIAERVKRTEIKDKSKVWNTARIEALELDNLIEVAKATLVSAEARKESRGAHA mOlO-1 KGVREVMAIAERVKRTEIKDKSKVNTARIEALELDNLIEVAKAT VSAEARKESRGAHA 490 500 510 520 530 540 550 560 570 580 aO10-1.pep SDDHPERDDEN MKHTLYHSDANTLSYKPVHTKPLSVEYIKPAKRVYX 1 1 1 1 1 ! 1 1 1 ! ! 1 1 1 1 ! 1 1 1 1 1 I I I I I 1 1 1 1 1 1 1 I I 1 1 1 1 1 1 1 1 I I 1 1 mOlO-l ΞDDHPERDDENWMKHTLYHSDINTLSYKPVHTKPLSVEYIKPAKRVYX 550 560 570 580 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 73>: gOll . seq 1 ATGAAGACAC ACCGCAAGAC CTGCTCTGCG GTGTGTTTTG CTTTTCAGAC 51 GGCATCGAAA CCCGCCGTTT CCATCCGACA TCCCAGCGAG GACATCATGA 101 GCCTGAAAAC CCGCCTTACC GAAGATATGA AAACCGCGAT GCGCGCCAAA 151 GATCAAGTTT CCCTCGGCAC CATCCGCCTC ATCAATGCCG CCGTCAAACA 201 GTTTGAAGTA GACGAACGCA CCGAAGCCGA CGATGCCAAA ATCACCGCCA 251 TCCTGACCAA AATGGTCAAA CAGCGCAAAG ACGGCGCGAA AATCTACACT 301 GAAGCCGGCC GTCAGGATTT GGCAGACAAA GAAAACGCCG AAATCGACGT 351 GCTGCACCGC TACCTGCCGC AAATGCTCTC CGCCGGCGAA ATCCGCACCG 401 CCGTCGAAGC AGCCGTTGCC GAAACCGGCG CGGCAGGTAT GGCGGATATG 451 GGCAAAGTGA TGGTCGTATT GAAAAcccGC CTCGCCGGCA AAGccgATAT 501 GGGCGAAGTC AACAAAATCT TGAAAAccGt aCTGACCGCC tga This corresponds to the amino acid sequence <SEQ ID 74; ORF 01 l.ng>: gOll.pep 1 MKTHRKTCSA VCFAFQTASK PAVSIRHPSE DIMSLKTRLT EDMKTAMRAK 51 DQVSLGTIRL IΝAAVKQFEV DERTEADDAK ITAILTKMVK QRKDGAKIYT 101 EAGRQDLADK EΝAEIDVLHR YLPQMLSAGE IRTAVEAAVA ETGAAGMADM 151 GKVMVVLKTR LAGKADMGEV ΝKILKTVLTA * The following partial DΝA sequence was identified in N meningitidis <SEQ ID 75>: mOll.seq (partial) 1 ATGAGGACAC ACCGCAAGAC CTGCTCTGCG GTGTGTTTTG CTTTTCAGAC 51 GGCATCGAAA CCCGCCGTTT CCATCCGACA TCCCAGCGAG GACATCATGA 101 GCCTGAAAAT CCGCCTTACC GAAGACATGA AAACCGCGAT GCGCGCCAAA 151 GACCAAGTTT CCCTCGGCAC CATCCGCCTC ATCAACGCCG CCGTCAAACA 201 GTTTGAAGTG GACGAACGCA CCGAAGCCGA CGATGCCAAA ATCACCGCCA 251 TCCTGACCAA AATGGTCAAA CAGCGAAAAG ACAGCGCGAA AATCTACACT 301 GAAGCCGGCC GTCAGGATTT GGCAGACAAA GAAAACGCCG AAATCGAGGT 351 ACTGCACCGC TACCTTCCCC AAATGCTTTC CGCCGGCGAA ATCCGTACCG 401 AGGTCGAAGC TGCCGTTGCC GAAACCGGCG CGGCAGGTAT GGCGGATATG 451 GGTAAAGTCA TGGGGCTGCT GAAAACCCGC CTCGCAGGTA AAGCCGA... This corresponds to the amino acid sequence <SEQ ID 76; ORF 011>: m011. ep (partial) 1 MRTHRKTCSA VCFAFQTASK PAVSIRHPSE DIMSLKIRLT EDMKTAMRAK 51 DQVSLGTIRL IΝAAVKQFEV DERTEADDAK ITAILTKMVK QRKDSAKIYT 101 EAGRQDLADK EΝAEIEVLHR YLPQMLSAGE IRTEVEAAVA ETGAAGMADM 151 GKVMGLLKTR LAGKA Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 011 shows 95.8% identity over a 165 aa overlap with a predicted ORF (ORF 01 l.ng) from N gonorrhoeae: mOll/gOll 10 20 30 40 50 60 Oll.pep MRTHRKTCSAVCFAFQTASKPAVSIRHPSEDIMSLKIRLTEDMKTAMRAKDQVSLGTIRL I : I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I II gOll MKTHRKTCSAVCFAFQTASKPAVSIRHPSEDIMSLKTRLTEDMKTAMRAKDQVSLGTIRL 10 20 30 40 50 60 70 80 90 100 110 120 mOll.pep IΝAAVKQFEVDERTEADDAKITAILTKMVKQRKDSAKIYTEAGRQDLADKEΝAEIEVLHR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I II I I I : I I I I gOll IΝAAVKQFEVDERTEADDAKITAILTKMVKQRKDGAKIYTEAGRQDLADKEΝAEIDVLHR 70 80 90 100 110 120 130 140 150 160 mOll . pep YLPQMLSAGEIRTEVEAAVAETGAAGMADMGKVMGLLKTRLAGKA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I gOll YLPQMLSAGEIRTAVEAAVAETGAAGMADMGKVMVVLKTRLAGKADMGEVNKILKTVLTA 130 140 150 160 170 180 gOll X The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 77>: g012.seq 1 ATGCTCGCCC GTCGCTATTT TTTCAATATC CAACCCGGGG CGGTTTTCAC 51 TGACAAACTG CTTGAACAAC TGATGCGTTT CCTCCAGTTC CTGCCGGAAT 101 TTCTGTTTGC CCTTTTCCGT ATTTTCACCC ATAAAAGTAA CCGTGCGCTT 151 AAATTCGCCC GCCGTCATCA CATCCACATC AATATCATGT TTTTTCAACa 201 gGcggTGGAT ATTCGgcact tccgCcacca cacccaccga accgatgacc 251 gcaaacggaG CGGAAACAAT TTTATCCGCc acacacgcca tcatatagcc 301 gcCGCTTGCC GCGACCTTAT CGAcggcgεc ggTCAGCGGA ATATTGCGTT 351 CGCGCAAACG CCTAAGCTGC GAAGCCGCCA AACCGTAACC GTGAACCACG 401 CCGCCCGGAC TTTCCAATCT GAGCAGAACC TCATCTTCAG GCTTGGCAAT 451 CAAAAGCACC GCCGTAATCT CATGACGCAA GGATTCTACG GCGTGTGCAT 501 ACAAATCGCC GTCAAAATCC AACACAAAAA GGCGGGATTT TTGCGTTTCG 551 GCAGATTTCT CCCCGCCCTC CTTCAAACGC TTTTTCTCTG CTTTGGCTTC 601 CGCCTTTTCC TTTTTCTTTT CTTTTTTTTC CTGATGTTTT GTCTCTTCCT 651 CGCTTAA This corresponds to the amino acid sequence <SEQ ID 78; ORF 012.ng>: g012.pep 1 MLARRYFFΝI QPGAVFTDKL LEQLMRFLQF LPEFLFALFR IFTHKSΝRAL 51 KFARRHHIHI ΝIMFFQQAVD IRHFRHHTHR TDDRKRSGΝΝ FIRHTRHHIA 101 AACRDLIDGD GQRΝIAFAQT PKLRSRQTVT VΝHAARTFQS EQΝLIFRLGΝ 151 QKHRRΝLMTQ GFYGVCIQIA VKIQHKKAGF LRFGRFLPAL LQTLFLCFGF 201 RLFLFLFFFF LMFCLFLA* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 79>: m012. seq 1 ATGCTCGCCC GTTGCCACTT CCTCAATATC CAATTGAGGG CGGTTCTCGC 51 TGACAAACTG CTTGAACAAC TGATGCGTTT CCTCCAGTTC CTGTCGGAAT 101 TTCTGTTTGC CCTTTTCCGT ATTTTCACCC ATAAAAGTAA CCGTGCGCTT 151 AAATTCGCCC GCCGTCATCA CATCCACATC AATATCATGT TTTTTCAACA 201 GGCGGTGGAT ATTCGGTACT TCCGCCACCA CACCCACCGA ACCGACAATC 251 GCAAACGGAG CGGAAGCAAT TTTATCCGCC ACACACGCCA TCATATAACC 301 GCCGCTCGCn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 351 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 451 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 501 nnnnnnnnnn nnnnnnnnnC AACACAAAAA GGCGTGATTT nTGCGTTTCG 551 GCAGATTTCT CCCCACCCTC CTTCAAACGT TTTTCcTCTG CTTTGGCTTC 601 CGCCTTTTCC TTTTTCTTTT CCTCTTTTTC CTGATGTTGT GCCTCTTCCC 651 CGCTTAA This corresponds to the amino acid sequence <SEQ ID 80; ORF 012>: m012.pep 1 MLARCHFLΝI QLRAVLADKL LEQLMRFLQF LSEFLFALFR IFTHKSΝRAL 51 KFARRHHIHI ΝIMFFQQAVD IRYFRHHTHR TDΝRKRSGSΝ FIRHTRHHIT 101 AARXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX 151 XXXXXXXXXX XXXXXXXXXX XXXQHKKA*F XRFGRFLPTL LQTFFLCFGF 201 RLFLFLFLFF LMLCLFPA* The following partial DNA sequence was identified in N meningitidis <SEQ ID 81>: a012. seq 1 ATGCTCGCCC GTTGCCACTT CCTCAATATC CAATTGAGGG CGGTTCTCGC 51 TGACAAACTG CTTGAACAAC TGATGCGTTT CCTCCAGTTC CTGTCGGAAT 101 TTCTGTTTGC CCTTTTCCGT ATTTTCACCC ATAAAAGTAA CCGTGCGCTT 151 AAATTCGCCC GCCGTCATCA CATCCACATC AATATCATGT TTTTTCAACA 201 GGCGGTGGAT ATTCGGTACT TCCGCTACAA CACCCACCGA ACCGACAATC 251 GCAAACGGAG CGGAAACAAT TTTATCCGCC ACACACGCCA TCATATAACC 301 ACCGCTCGCC GCCACCTTAT CGACGGCGAC GGTCAGCGGA ATATTGCGTT 351 CGCGCAAACG CCTAAGCTGC GAAGCCGCCA AACCGTAACC GTGAACCACG 401 CCGCCCGGAC TTTCCAATCT AAGCAGAACC TCATCTTCAG GCTTGGCAAT 451 CAAAAGCACC GCCGTAATCT CATGACGCAA GGATTCTACG GCGTGTGCAT 501 ACAAATCGCC GTCAAAATCC AACACAAAAA GGCGGGATTT TTGCGTTTCG 551 GAAGATTTCT CCCCACCCTC CTTCAAACGC TTTTTCTCTG CTTTGGCTTC 601 CGCCTTTTCC TTTTTCTTTT CCTCTTTTTC CTGATGTTTT GCCTCTTCCC 651 CGCTTAA This corresponds to the amino acid sequence <SEQ ID 82; ORF 012. a>: a012.pep 1 MLARCHFLΝI QLRAVLADKL LEQLMRFLQF LSEFLFALFR IFTHKSΝRAL 51 KFARRHHIHI ΝIMFFQQAVD IRYFRYΝTHR TDΝRKRSGΝΝ FIRHTRHHIT 101 TARRHLIDGD GQRΝIAFAQT PKLRSRQTVT VΝHAARTFQS KQΝLIFRLGΝ 151 QKHRRΝLMTQ GFYGVCIQIA VKIQHKKAGF LRFGRFLPTL LQTLFLCFGF 201 RLFLFLFLFF LMFCLFPA* moi2/aoi2 64.2%> identity over a 218 aa overlap 10 20 30 40 50 60 m012. pep MLARCHFLΝIQLRAVLADKLLEQLMRFLQFLSEFLFALFRIFTHKSΝRALKFARRHHIHI I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a012 MLARCHFLΝIQLRAVLADKLLEQLMRFLQFLΞEFLFALFRIFTHKSΝRALKFARRHHIHI 10 20 30 40 50 60 70 80 90 100 110 120 m012.pep ΝIMFFQQAVDIRYFRHHTHRTDΝRKRSGSΝFIRHTRHHITAARXXXXXXXXXXXXXXXXX I I I I I I I I I I I I I I I :: I I I I I I I I I I I : I I I I I I I I I I I : I I : a012 ΝIMFFQQAVDIRYFRYΝTHRTDΝRKRSGΝΝFIRHTRHHITTARRHLIDGDGQRΝIAFAQT 70 80 90 100 110 120 130 140 150 160 170 180 mO12. pep XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQHKKAXF : : : : lllll l a012 PKLRSRQTVTVΝHAARTFQSKQΝLIFRLGΝQKHRRΝLMTQGFYGVCIQIAVKIQHKKAGF 130 140 150 160 170 180 190 200 210 219 m012.pep XRFGRFLPTLLQTFFLCFGFRLFLFLFLFFLMLCLFPAX I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I : I I I I I I a012 LRFGRFLPTLLQTLFLCFGFRLFLFLFLFFLMFCLFPAX 190 200 210 Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 012 shows 58.7% identity over a 218 aa overlap with a predicted ORF (ORF 012.ng) from N gonorrhoeae: m012/g012 10 20 30 40 50 60 m012.pep MLARCHFLΝIQLRAVLADKLLEQLMRFLQFLSEFLFALFRIFTHKSΝRALKFARRHHIHI I I I I : I: I I I I I :: I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I g012 MLARRYFFΝIQPGAVFTDKLLEQLMRFLQFLPEFLFALFRIFTHKSΝRALKFARRHHIHI 10 20 30 40 50 60 70 80 90 100 110 120 m012. pep NIMFFQQAVDIRYFRHHTHRTDNRKRSGSNFIRHTRHHITAARXXXXXXXXXXXXXXXXX I I I I I I I I I I I I : I I I I I I I I I : I I I I I : I I I I I I I I I I : I I : g012 NIMFFQQAVDIRHFRHHTHRTDDRKRSGNNFIRHTRHHIAAACRDLIDGDGQRNIAFAQT 70 80 90 100 110 120 130 140 150 160 170 180 m012. pep XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQHKKAXF : : : : lllll l g012 PKLRSRQTVTVNHAARTFQSEQNLIFRLGNQKHRRNLMTQGFYGVCIQIAVKIQHKKAGF 130 140 150 160 170 180 190 200 210 219 m012.pep XRFGRFLPTLLQTFFLCFGFRLFLFLFLFFLMLCLFPAX I I I I I I I : I I I I : I I I I I I I I I I I I I : I I I I : I I I II g012 LRFGRFLPALLQTLFLCFGFRLFLFLFFFFLMFCLFLAX 190 200 210 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 83>: m012-l.seq 1 ATGCTCGCCC GTTGCCACTT CCTCAATATC CAATTGAGGG CGGTTCTCGC 51 TGACAAACTG CTTGAACAAC TGATGCGTTT CCTCCAGTTC CTGTCGGAAT 101 TTCTGTTTGC CCTTTTCCGT ATTTTCACCC ATAAAAGTAA CCGTGCGCTT 151 AAATTCGCCC GCCGTCATCA CATCCACATC AATATCATGT TTTTTCAACA 201 GGCGGTGGAT ATTCGGTACT TCCGCCACCA CACCCACCGA ACCGACAATC 251 GCAAACGGAG CGGAAGCAAT TTTATCCGCC ACACACGCCA TCATATAACC 301 GCCGCTCGCC GCCACCTTAT CGACGGCGAC GGTCAGCGGA ATATTGCGTT 351 CGCGCAAACG CyTAAGCTGC GAAGCCGCCA AACCGTAACC GTGAACCACG 401 CCGCCCGGAC TTTCCAATCT GAGCAGAACC TCATCTTCAG GCTTGGCAAT 451 CAAAAGCACC GCCGTAATCT CATGACGCAA GGATTCTACG GCGTGTGCAT 501 ACAAATCGCC GTCAAAATCC AACACAAAAA GGCGGGATTT TTGCGTTTCG 551 GCAGATTTCT CCCCACCCTC CTTCAAACGC TTTTTCTCTG CTTTGGCTTC 601 CGCCTTTTCC TTTTTCTTTT CCTCTTTTTC CTGATGTTTT GCCTCTTCCC 651 CGCTTAA This corresponds to the amino acid sequence <SEQ ID 84; ORF 012-1>: m012-l .pep 1 MLARCHFLΝI QLRAVLADKL LEQLMRFLQF LSEFLFALFR IFTHKSΝRAL 51 KFARRHHIHI ΝIMFFQQAVD IRYFRHHTHR TDΝRKRSGSΝ FIRHTRHHIT 101 AARRHLIDGD GQRΝIAFAQT XKLRSRQTVT VΝHAARTFQS EQΝLIFRLGΝ 151 QKHRRΝLMTQ GFYGVCIQIA VKIQHKKAGF LRFGRFLPTL LQTLFLCFGF 201 RLFLFLFLFF LMFCLFPA* m012-l/g012 91.7% identity in 218 aa overlap 10 20 30 40 50 60 m012-l . pep MLARCHFLΝIQLRAVLADKLLEQLMRFLQFLSEFLFALFRIFTHKSΝRALKFARRHHIHI I I I I : I : I I I I I :: I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I II I g012 MLARRYFFΝIQPGAVFTDKLLEQLMRFLQFLPEFLFALFRIFTHKSΝRALKFARRHHIHI 10 20 30 40 50 60 70 80 90 100 110 120 m012-l . pep ΝIMFFQQAVDIRYFRHHTHRTDΝRKRSGSΝFIRHTRHHITAARRHLIDGDGQRΝIAFAQT I I I I I I I I I I I I : I I I I I I I I I : I I I I I : I I I I I I I I I I : I I I I I I I I I I I I I I I I I I g012 ΝIMFFQQAVDIRHFRHHTHRTDDRKRSGΝΝFIRHTRHHIAAACRDLIDGDGQRΝIAFAQT 70 80 90 100 110 120 130 140 150 160 170 180 m012-l.pep XKLRSRQTVTVΝHAARTFQSEQΝLIFRLGΝQKHRRΝLMTQGFYGVCIQIAVKIQHKKAGF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I g012 PKLRSRQTVTVΝHAARTFQSEQΝLIFRLGΝQKHRRΝLMTQGFYGVCIQIAVKIQHKKAGF 130 140 150 160 170 180 190 200 210 219 m012-l . pep LRFGRFLPTLLQTLFLCFGFRLFLFLFLFFLMFCLFPAX I I I I I I I I : I I I I I I I I I I I I I I I I I I : I I I I I I I I I I g012 LRFGRFLPALLQTLFLCFGFRLFLFLFFFFLMFCLFLAX 190 200 210 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 85>: a012-l.seq 1 ATGCTCGCCC GTTGCCACTT CCTCAATATC CAATTGAGGG CGGTTCTCGC 51 TGACAAACTG CTTGAACAAC TGATGCGTTT CCTCCAGTTC CTGTCGGAAT 101 TTCTGTTTGC CCTTTTCCGT ATTTTCACCC ATAAAAGTAA CCGTGCGCTT 151 AAATTCGCCC GCCGTCATCA CATCCACATC AATATCATGT TTTTTCAACA 201 GGCGGTGGAT ATTCGGTACT TCCGCTACAA CACCCACCGA ACCGACAATC 251 GCAAACGGAG CGGAAACAAT TTTATCCGCC ACACACGCCA TCATATAACC 301 ACCGCTCGCC GCCACCTTAT CGACGGCGAC GGTCAGCGGA ATATTGCGTT 351 CGCGCAAACG CCTAAGCTGC GAAGCCGCCA AACCGTAACC GTGAACCACG 401 CCGCCCGGAC TTTCCAATCT AAGCAGAACC TCATCTTCAG GCTTGGCAAT 451 CAAAAGCACC GCCGTAATCT CATGACGCAA GGATTCTACG GCGTGTGCAT 501 ACAAATCGCC GTCAAAATCC AACACAAAAA GGCGGGATTT TTGCGTTTCG 551 GAAGATTTCT CCCCACCCTC CTTCAAACGC TTTTTCTCTG CTTTGGCTTC 601 CGCCTTTTCC TTTTTCTTTT CCTCTTTTTC CTGATGTTTT GCCTCTTCCC 651 CGCTTAA This corresponds to the amino acid sequence <SEQ ID 86; ORF 012-l.a>: a012-l.pep 1 MLARCHFLΝI QLRAVLADKL LEQLMRFLQF LSEFLFALFR IFTHKSΝRAL 51 KFARRHHIHI ΝIMFFQQAVD IRYFRYΝTHR TDΝRKRSGΝΝ FIRHTRHHIT 101 TARRHLIDGD GQRΝIAFAQT PKLRSRQTVT VΝHAARTFQS KQΝLIFRLGΝ 151 QKHRRΝLMTQ GFYGVCIQIA VKIQHKKAGF LRFGRFLPTL LQTLFLCFGF 201 RLFLFLFLFF LMFCLFPA* a012-l/m012-l 97.2% identity in 218 aa overlap 10 20 30 40 50 60 a012-l.pep MLARCHFLΝIQLRAVLADKLLEQLMRFLQFLSEFLFALFRIFTHKSΝRALKFARRHHIHI I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m012-l MLARCHFLΝIQLRAVLADKLLEQLMRFLQFLSEFLFALFRIFTHKSΝRALKFARRHHIHI 10 20 30 40 50 60 70 80 90 100 110 120 a012-l . pep ΝIMFFQQAVDIRYFRYΝTHRTDΝRKRSGΝΝFIRHTRHHITTARRHLIDGDGQRΝIAFAQT I I I I I I I I I I I I I II : : I I I I I I I I I I I : I I I I I I I I I II : I I I I I I I I I I I I I I I I I II m012-l ΝIMFFQQAVDIRYFRHHTHRTDΝRKRSGSΝFIRHTRHHITAARRHLIDGDGQRΝIAFAQT 70 80 90 100 110 120 130 140 150 160 170 180 a012-l.pep PKLRSRQTVTVΝHAARTFQSKQΝLIFRLGΝQKHRRΝLMTQGFYGVCIQIAVKIQHKKAGF I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m012-l XKLRSRQTVTVΝHAARTFQSEQΝLIFRLGΝQKHRRΝLMTQGFYGVCIQIAVKIQHKKAGF 130 140 150 160 170 180 190 200 210 219 a012-l .pep LRFGRFLPTLLQTLFLCFGFRLFLFLFLFFLMFCLFPAX I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I m012-l LRFGRFLPTLLQTLFLCFGFRLFLFLFLFFLMFCLFPAX 190 200 210 The following partial DΝA sequence was identified in N. gonorrhoeae <SEQ ID 87>: g013.seq 1 aTgcctttga ccatgctgtg cagcaGGAcg tGCGGTTtgt tcataataca 51 gtCcgaccGG AAAagcggAG GAAaCGCAGT GCCGCGCCCT TCCCCTTTCT 101 TGCCGTGGCA GGCGATGCag tTgGATTCGT ACACTTTTTG CCCTTTtGtc 151 atgatGCTgt tgtcggCGGC AGAAGCgGCG GcgCAGAGGC AGCACAAGAT 201 GAAGGCGGTC GGCAGTCGGG TTGTGTtcat tGgcgTTTCC cctaatgttt 251 tgaaaccttg ttttttgatt Ttgcctttac ggggtgaaaa gtttttTtgg 301 cccaaatccg gaatttag This corresponds to the amino acid sequence <SEQ ID 88; ORF 013.ng: g013.pep 1 MPLTMLCSRT CGLFIIQSDR KSGGNAVPRP SPFLPWQAMQ LDSYTFCPFV 51 MMLLSAAEAA AQRQHKMKAV GSRWFIGVS PNVLKPCFLI LPLRGEKFFW 101 PKSGI* The following partial DNA sequence was identified in N meningitidis <SEQ ID 89>: 013.seq 1 ATGCCTTTGA CCATGCTGTG CAGCAGCACC TGCGGTTTTT TCATGATGAA 51 GTCGGAGCGG TAGAGCGGCG GAAACATGGT TCCGCGGCCT TCGCCCTTTT 101 TGCCGTGGCA GGCGACGCAG TTGGATTCGT ACACTTTTTG CCCTTTTGTC 151 ATGATGCTGT TGTCGGCGGC AGAAGCGGCG GCGCAGAAGC AGCCCAAGAC 201 GAGGGCGGTC GGCAGTCGGG TTGTGTTCAT TGGTGTTTCC TTCATGTTTG 251 AAACCTTGTT GTTGATTTTG CGTAGCGGGT GAAAGATTTT TTTGCCGAAT 301 CAGTAG This corresponds to the amino acid sequence <SEQ ID 90; ORF 013>: m013.pep 1 MPLTMLCSST CGFFMMKSER XSGGΝMVPRP SPFLPWQATQ LDSYTFCPFV 51 MMLLSAAEAA AQKQPKTRAV GSRWFIGVS FMFETLLLIL RSGXKIFLPΝ 101 Q* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 91>: a013. seq 1 ATGCCTTTGA CCATGCTGTG CAGCAGCACC TGCGGTTTTT TCATGATGAA 51 GTCGGAGCGG TAGAGCGGCG GAAACATGGT TCCGCGGCCT TCGCCCTTTT 101 TGCCGTGGCA GGCGACGCAG TTGGATTCGT ACACTTTTTG CCCTTTTGTC 151 ATGATGCTGT TGTCGGCGGC AGAAGCGGCG GCGCAGAGGC AGCCCAAGAC 201 GAGGGCGGTC GGCAGTCGGG TTGTGTTCAT TGGTGTTTCC TTAATGTTTG 251 AAACCTTGTT GTTGATTTTG CGTAGCGGGT GAAAGATTTT CTTGCCGAAT 301 CGGTAG This corresponds to the amino acid sequence <SEQ ID 92; ORF 013. a>: a013.pep 1 MPLTMLCSST CGFFMMKSER *SGGΝMVPRP SPFLPWQATQ LDSYTFCPFV 51 MMLLSAAEAA AQRQPKTRAV GSRWFIGVS LMFETLLLIL RSG*KIFLPN 101 R* oi3/aoi3 91.0% identity over a 101 aa overlap 10 20 30 40 50 60 m013.pep MPLTMLCSSTCGFFMMKSERXSGGNMVPRPSPFLPWQATQLDSYTFCPFVMMLLSAAEAA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a013 MPLTMLCSSTCGFFMMKSERXSGGNMVPRPSPFLPWQATQLDSYTFCPFVMMLLSAAEAA 10 20 30 40 50 60 70 80 90 100 m013.pep AQKQPKTRAVGSRWFIGVSFMFETLLLILRSGXKIFLPNQX I I : I I I I I I I I I II I I I I I I : I I I I I I I I I I I II I I I I I I : I a013 AQRQPKTRAVGSRWFIGVSLMFETLLLILRSGXKIFLPNRX 70 80 90 100 Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 013 shows 73.3%> identity over a 101 aa overlap with a predicted ORF (ORF 013.ng) from N. gonorrhoeae: m013/g013 10 20 30 40 50 60 m013 . pep MPLTMLCSSTCGFFMMKSERXSGGNMVPRPSPFLPWQATQLDSYTFCPFVMMLLSAAEAA I I I I I I I I I I I : I : : : I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g013 MPLTMLCSRTCGLFIIQSDRKSGGNAVPRPSPFLPWQAMQLDSYTFCPFVMMLLSAAEAA 10 20 30 40 50 60 70 80 90 100 m013. pep AQKQPKTRAVGSRWFIGVSF-MFETLLLILR-SGXKIFLPNQX I I : I I : I I I I I I I I I I I I : : : : I I I I I : I I : g013 AQRQHKMKAVGSRWFIGVSPNVLKPCFLILPLRGEKFFWPKSGIX 70 80 90 100 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 93>: g015. seq 1 ATGCAGTATC TGATTGTCAA ATACAGCCAT CAAATCTTCG TTACCATCAC 51 CATTTTGGTA TTCAACATCC GTTTTTTCCT ACTTTGGAAA AATCCAGAAA 101 AGCCCTTGGT CGGCTTTTGG AAAGCACTGC CCCACCTCAA CGACACGATG 151 CTGCTGTTTA CGGGATTGTG GCTGATGAAG ATTACCCATT TCTCCCCGTT 201 CAACGCGCCT TGGCTCGGCA CAAAAATCCT GCTCCTGTTC GCCTACATCG 251 CACTGGGCAT GGTAATGATG CGCGCCCGTC CGCGTTCGAC CAAGTTCTAC 301 ACCGTTTACC TGCTCGCTAT GTGTTGCATC GCCTGCATCG TTTACCTTGC 351 CAAAACCAAA GTCCTGCCAT TCTGA This corresponds to the amino acid sequence <SEQ ID 94; ORF 015.ng>: g015.pep 1 MQYLIVKYSH QIFVTITILV FΝIRFFLLWK ΝPEKPLVGFW KALPHLΝDTM 51 LLFTGLWLMK ITHFSPFΝAP WLGTKILLLF AYIALGMVMM RARPRSTKFY 101 TVYLLAMCCI ACIVYLAKTK VLPF* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 95>: m015.seq (partial) 1 ..AAAATCAGAA AAGCCTTGGC GGGCTTTTGG AAGGCACTGC CCCACCTTAA 51 CGACACCATG CTGCTGTTTA CGGGATTGTG GCTGATGAAA ATTACCCATT 101 TCTCCCCGTT CAACGCGCCT TGGCTCGGTA CAAAAATCCT GCTTCTGCTC 151 GCCTATATCG CATTGGGTAT GATGATGATG CGCGCCCGTC CGCGTTCGAC 201 CAAGTTCTAC ACCGTTTACC TGCTCGCCAT GTGTTGCGTC GCCTGCATCG 251 TTTACCTTGC CAAAACCAAA GTCCTGCCTT TCTGA This corresponds to the amino acid sequence <SEQ ID 96; ORF 015: m015 . pep (partial ) 1 ..KIRKALAGFW KALPHLΝDTM LLFTGLWLMK ITHFSPFΝAP WLGTKILLLL 51 AYIALGMMMM RARPRSTKFY TVYLLAMCCV ACIVYLAKTK VLPF* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 97>: a015. seq 1 ATGCAGTATC TGATTGTCAA ATACAGCCAT CAAATCTTCG TTACCATCAC 51 CATTTTGGTA TTCAACATCC GTGTTTTCΝT ACTTTGGAAA AATCCAGAAA 101 AGCCCTTGGC GGGCTTTTGG AAGGCACTGC CCCACCTTAA CGACACCATG 151 CTGCTGTTTA CGGGATTGTG GCTGATGAAA ATTACCCATT TCTCCCCGTT 201 CAACGCGCCT TGGCTCGGTA CAAAAATCCT GCTTCTGCTC GCCTATATCG 251 CATTGGGTAT GATGATGATG CGCGCCCGTC CGCGTTCGAC CAAGTTCTAC 301 ACCGTTTACC TGCTCGCCAT GTGTTGCCTC ACCTGCATCG TTTACCTTGC 351 CAAAACCAAA GTCCTGCCTT TCTGA This corresponds to the amino acid sequence <SEQ ID 98; ORF 015.a>: a015 . pep 1 MQYLIVKYSH QIFVTITILV FNIRVFXLWK NPEKPLAGFW KALPHLNDTM 51 LLFTGLWLMK ITHFSPFNAP WLGTKILLLL AYIALGMMMM RARPRSTKFY 101 TVYLLAMCCL TCIVYLAKTK VLPF* moi5/aoi5 96.1% identity over a 91 aa overlap 10 20 30 m015.pep KIRKALAGFWKALPHLNDTMLLFTGLWLMKITH I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a015 LIVKYSHQIFVTITILVFNIRVFXLWKNPEKPLAGFWKALPHLNDTMLLFTGLWLMKITH 10 20 30 40 50 60 40 50 60 70 80 90 m015. pep FSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTVYLLAMCCVACIVYLAKTKVLP I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : : I I I I I I I I I I I I a015 FSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTVYLLAMCCLTCIVYLAKTKVLP 70 80 90 100 110 120 m015.pep FX I I a015 FX Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 015 shows 94.5% identity over a 91 aa overlap with a predicted ORF (ORF 015.ng) from N gonorrhoeae: m015/g015 10 20 30 m015.pep KIRKALAGFWKALPHLΝDTMLLFTGLWLMKITH I g015 LIVKYSHQIFVTITILVFΝIRFFLLWKΝPEKPLVGFWKALPHLΝDTMLLFTGLWLMKITH 10 20 30 40 50 60 40 50 60 70 80 90 m015.pep FSPFΝAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTVYLLAMCCVACIVYLAKTKVLP g015 FSPFΝAPWLGTKILLLFAYIALGMVMMRARPRSTKFYTVYLLAMCCIACIVYLAKTKVLP 70 80 90 100 110 120 m015.pep FX II g015 FX The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 99>: gOlδ.seq 1 atGCAGCAGG GGCagttggt tggacgcgtc gcccgcaata AAGATATGCG 51 GAATgctggt CTGCATggtC AGCGGATCGG CAACGGGtac gccgcgcgcg 101 tctttgTCGA TATTGATGTT TTCCAAACCG ATATtgTCAA CGTTCGGACG 151 GCgACCTACG GCTGCCAACA TATATTCGGC AACAAATACG CCTTTTTCGC 201 CATCCTGCTC CCAATGGACT tctACATTGC CGTCTGCGTC GAGTTTGACC 251 TCGGTTTTAG CATCCAGATG CAGTTTCAAT tctTCTCCGA ACACGGCTTT 301 CGCCTCGTCT GA This corresponds to the amino acid sequence <SEQ ID 100; ORF 018.ng>: g018.pep 1 MQQGQLVGRV ARΝKDMRΝAG LHGQRIGΝGY AARVFVDIDV FQTDIVΝVRT 51 ATYGCQHIFG ΝKYAFFAILL PMDFYIAVCV EFDLGFSIQM QFQFFSEHGF 101 RLV* The following partial DNA sequence was identified in N meningitidis <SEQ ID 101>: mOlδ.seq 1 ATGCAGCAGA GGCAGTTGGT TGGACGCATC GCCTGCGATG AAGATATGCG 51 GAATACTGGT CTGCATGGTC AGCGGGTCGG CAACAGGTAC GCCGCGCGCA 101 TCTTTTTCGA TATTGATATT TTCCAAACCG ATATTGTCAA CGTTCGGACG 151 GCGGCCCACG GCTGCCAGCA TATATTCGGC AACAAATACG CCTTTTTCGC 201 CATCCTGCTC CCAATGGACT TCTACATTGC CGTCTGCATC GAGTTTGACC 251 TCGGTTTTAG CATCCAGATG CAGTTTCAAT TCTTCGCCGA ACACGGCGTT 301 CGCCTCGTCT GA This corresponds to the amino acid sequence <SEQ ID 102; ORF 018>: mOlβ.pep 1 MQQRQLVGRI ACDEDMRΝTG LHGQRVGΝRY AARIFFDIDI FQTDIVΝVRT 51 AAHGCQHIFG ΝKYAFFAILL PMDFYIAVCI EFDLGFSIQM QFQFFAEHGV 101 RLV* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 103>: a018. seq 1 ATGCAGCAGG GGCAGTTGGT TGGACGCGTC GCCCGCAATA AAGATATGCG 51 GAATACTGGT CTGCATAGTC AGCGGATCGG CAACGGGTAC GCCGCGCGCA 101 TCTTTTTCGA TATTGATGTT TTCCAAACCG ATATTGTCAA CGTTCGGACG 151 GCGGCCTACG GCTGCCAGCA TATATTCGGC AACAAATACG CCTTTTTCGC 201 CATCCTGCTC CCAATGGACT TCTACATTGC CGTCTGCGTC GAGTTTGGCC 251 TCGGTTTTAG CATCCAAATG CAGTTTCAAT TCTTCACCGA ACACGGCTTT 301 CGCCTCGTCT GA This corresponds to the amino acid sequence <SEQ ID 104; ORF 018. a>: a018.pep 1 MQQGQLVGRV ARΝKDMRΝTG LHSQRIGΝGY AARIFFDIDV FQTDIVΝVRT 51 AAYGCQHIFG ΝKYAFFAILL PMDFYIAVCV EFGLGFSIQM QFQFFTEHGF 101 RLV* moi8/aoi8 86.4%> identity over a 103 aa overlap 10 20 30 40 50 60 m018. pep MQQRQLVGRIACDEDMRΝTGLHGQRVGΝRYAARIFFDIDIFQTDIVΝVRTAAHGCQHIFG III I I I I I : I :: I I I I I I I I : I I : I I I II I I I I I I I : I I I I I I I I I I I I : I I I I I I I a018 MQQGQLVGRVARΝKDMRΝTGLHSQRIGΝGYAARIFFDIDVFQTDIVΝVRTAAYGCQHIFG 10 20 30 40 50 60 70 80 90 100 m018. pep ΝKYAFFAILLPMDFYIAVCIEFDLGFSIQMQFQFFAEHGVRLVX I I I I I I I I I I I I I I I I I I I : I I 111111111111:111 I I I I a018 ΝKYAFFAILLPMDFYIAVCVEFGLGFSIQMQFQFFTEHGFRLVX 70 80 90 100 Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 018 shows 84.5% identity over a 103 aa overlap with a predicted ORF (ORF 018.ng) from N gonorrhoeae: m018/g018 10 20 30 40 50 60 m018. pep MQQRQLVGRIACDEDMRΝTGLHGQRVGΝRYAARIFFDIDIFQTDIVΝVRTAAHGCQHIFG III I I I I I : I :: I I I I : I I I I I I : I I I I I I: I I I I : I I I I I I I I I I I :: I I I I | | | g018 MQQGQLVGRVARΝKDMRΝAGLHGQRIGΝGYAARVFVDIDVFQTDIVΝVRTATYGCQHIFG 10 20 30 40 50 60 70 80 90 100 m018.pep NKYAFFAILLPMDFYIAVCIEFDLGFSIQMQFQFFAEHGVRLVX I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I : I I I I I I I g018 NKYAFFAILLPMDFYIAVCVEFDLGFSIQMQFQFFSEHGFRLVX 70 80 90 100 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 105>: g019.seq (partial) 1 ..ctgctggcgg ccctggtgct tgccgcgtgt tcttcgACAA ACAcacTGCC 51 AGCCGGCAAG ACCCCGGCAG ACAATATAGA AActgcCgAC CTTTCGGCAA 101 GCGTTCCCAC ccgcCCTGCC GAACCGGAAG GAAAAACGCT GGCAGATTAC 151 GGCGGCTACC CGTCCGCACT GGATGCAGTG AAACAGAACA ACGATGCGGC 201 AGCCGCCGCC TATTTGGAAA Acgcaggaga cagCGcgatg gcGGAAAatg 251 tccgcaagga gtgGCTGa This corresponds to the amino acid sequence <SEQ ID 106; ORF 019.ng>: g019.pep (partial) 1 ..LLAALVLAAC SSTΝTLPAGK TPADΝIETAD LSASVPTRPA EPEGKTLADY 51 GGYPSALDAV KQΝΝDAAAAA YLEΝAGDSAM AEΝVRKEWL* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 107>: m019.seq (partial) 1 ATGTACCTAC CCTCTATGAA GCATTCCCTG CCGCTGCTGG CGGCCCTGGT 51 GCTTGCCGCG TGTTCTTCGA CAAACACACT GCCAGCCGGC AAGACCCCGG 101 CAGACAATAT AGAAACTGCC GACCTTTCGG CAAGCGTTCC CACCCGCCCT 151 GCCGAACCCG AAAGAAAAAC GCTGGCAGAT TACGGCGGCT ACCCGTCCGC 201 ACTGGATGCA GTGAAACAGA AAAACGATGC CGCCGTCGCC GCCTATTTGG 251 AAAACGCCGG CGACAGCGCG ATGGCGGAAA ATGTCCGCAA CGAGTGGCTG 301 AAGTCTTTGG GCGCACGCAG ACAGTGGACG CTGTTTGCAC AGGAATACGC 351 CAAACTCGAA CCGGCAGGGC GCGCCCAAGA AGTCGAATGC TACGCCGATT 401 CGAGCCGCAA CGACTATACG CGTGCCGCTG AACTGGTCAA AAATACGGGC 451 AAACTGCCTT CGGGCTGCAC CAAACTGTTG GAACAGGCAG CCGCATCCGG 501 CTTGTTGGAC GGCAACGACG CCTGGAGGCG CGTGCGCGGA CTGCTGGCCG 551 GCCGCCAAAC CACAGACGCA CGCAACCTTG CCGCCGCATT GGGCAGCCCG 601 TTTGACGGCG GTACACAAGG TTCGCGCGAA TATGCCCTGT TGAACGTCAT 651 CGGCAAAGAA GCACGCAAAT CGCCGAATGC CGCCGCCCTG CTGTCCGAAA 701 TGGAAAGCGG TTTAAGCCTC GAACAACGCA GTTTCGCGTG GGGCGTATTG 751 GGGCATTATC AGTCGCAAAA CCTCAATGTG CCTGCCGCCT TGGACTATTA 801 CGGCAAGGTT GCCGACCGCC GCCAACTGAC CGACGACCAA ATCGAGTGGT 851 ACGCCCGCGC CGCCTTGCGC GCCCGACGTT GGGACGAGCT GGCCTCCGTT 901 ATCTCGCATA TGCCCGAAAA ACTGCAAAAA AGCCCGACCT GGCTCTACTG 951 GCTGGCACGC AGCCGCGCCG CAACGGGCAA CACGCAAGAG GCGGAAAAAC 1001 TTTACAAACA GGCGGCAGCG ACGGGCAGGA ATTTTTATGC GGTGCTGGCA 1051 GGGGAAGAAT TGGGTCGGAA AATCGATACG CGCAACAATG TGCCCGATGC 1101 CGGCAAAAAC AGCGTCCGCC GCATGGCGGA AGACGGTGCA GTCAAACGCG 1151 CACTGGTACT GTTCCAAAAC AGCCAATCTG CCGGTGATGC AAAAATGCGC 1201 CGTCAGGCTC AGGCGGAATG GCGTTTTGCC ACACGCGGCT TTGACGAAGA 1251 CAAGCTGCTG ACCGCCGCGC AAACCGCGTT CGACCACGGT TTTTACGATA 1301 TGGCGGTCAA CAGCGCGGAA CGCACCGACC GCAAACTCAA CTACACCTTG 1351 CGCTATATTT CGCCGTTTAA AGACACGGTA ATCCGCCACG CGCAAAATGT 1401 TAATGTCGAT CCGGCTTGGG TTTATGGGCT GATTCGTCAG GAAAGCCGCT 1451 TCGTTATAGG CGCGCAATCC CGCGTAGGCG CGCAGGGGCT GATGCAGGTT 1501 ATGCCTGCCA CCGCGCGCGA AATCGCCGGC AAAATCGGTA TGGATGCCGC 1551 ACAACTTTAC ACCGCCGACG GG... This corresponds to the amino acid sequence <SEQ ID 108; ORF 019>: m019.pep (partial) 1 MYLPSMKHSL PLLAALVLAA CSSTΝTLPAG KTPADΝIETA DLSASVPTRP 51 AEPERKTLAD YGGYPSALDA VKQKNDAAVA AYLENAGDSA MAENVRNEWL 101 KSLGARRQWT LFAQEYAKLE PAGRAQEVEC YADSSRNDYT RAAELVKNTG 151 KLPSGCTKLL EQAAASGLLD GNDAWRRVRG LLAGRQTTDA RNLAAALGSP 201 FDGGTQGSRE YALLNVIGKE ARKSPNAAAL LSEMESGLSL EQRSFAWGVL 251 GHYQSQNLNV PAALDYYGKV ADRRQLTDDQ IEWYARAALR ARRWDELASV 301 ISHMPEKLQK SPTWLYWLAR SRAATGNTQE AEKLYKQAAA TGRNFYAVLA 351 GEELGRKIDT RNNVPDAGKN SVRRMAEDGA VKRALVLFQN SQSAGDAKMR 401 RQAQAEWRFA TRGFDEDKLL TAAQTAFDHG FYDMAVNSAE RTDRKLNYTL 451 RYISPFKDTV IRHAQNVNVD PAWVYG IRQ ESRFVIGAQS RVGAQGLMQV 501 MPATAREIAG KIGMDAAQLY TADG... The following partial DNA sequence was identified in N meningitidis <SEQ ID 109>: a019.seq 1 ATGTACCCAC CCTCTCTGAA GCATTCCCTG CCGCTGCTGG TGGΝCCTGGT 51 GCTTGCCGCG TGTTCTTNGA CAAACACACT GTCAGCCGAC AAGACCCCGG 101 CAGACAATAT AGAAACTGCC GACCTTTCGG CAAGCGTTCC CACCΝGCCCT 151 GCCGAACCCG AANGAAAAAC GTΝGGCAGAT TACGGCGGCT ACCCGTCCGC 201 ACTGGATGCA GTGAAACAGA AAAACGATGC CGCCGTCGCC GCCTATTTGG 251 AAAACGCCGG CGACAGCGCG ATGGCGGAAA ATGTCCGCAA CGAGTGGCTG 301 AAGTCTTTGG GCGCGCGCAG ACAGTGGACG CTGTΝTGCAC AΝGAATATGC 351 NAAACTCGAA CCGGCANGGC GCGCCCAAGA AGTCGAATGC TACGCCGATT 401 CGAGCCGCAA CGACTATACG CGTGCCGCCG AACTGGTCAA AAATACGGGC 451 AAACTGCCTT CGGGCTGCAC CAAACTGTTG GAACAGGCAG CCGCATCCGG 501 CTTGTTGGAC GGCAACGACG CCTGGAGGCG CGTGCGCGGA CTGCTGGCCG 551 GCCGCCAAAC CACAGACGCA CGCAACCTTG CCGCCGCATT GGGCAGCCCG 601 TTTGACGGCG GTACACAAGG TTCGCGCGAA TATGCCCTGT TGAACGTCAT 651 CGGCAAAGAA GCACGCAAAT CGCCGAATGC CGCCGCCCTG CTGTCCGAAA 701 TGGAAAGCGG TTTAAGCCTC GAACAACGCA GTTTCGCGTG GGGCGTATTG 751 GGGCATTATC AGTCGCAAAA CCTCAATGTG CCTGCCGCCT TGGACTATTA 801 NGGCAAGGTT GCCGACCGCC GCCAACTGAC CGACGACCAA ATCGAGTGGT 851 ACGCCCGCGC CGCNNTNNGC ΝΝΝCGΝΝGTT ΝGΝAΝGAΝΝT GGCΝΝCCGΝΝ 901 ANCNCGNNNN TGCNNGANAA ACΝΝΝΝΝΝAΝ AGΝCΝΝAΝΝT ΝGΝTΝΝAΝTG 951 NNTGGCACGC AGCCGCGCCG CΝACGGGCAA CACGCAAΝAΝ GCGGAΝAAAC 1001 TNTACAAACA GGCGGCAGCA ΝCGGGCAΝGA ATTTTTATGC ΝGTGCTGΝCΝ 1051 GGGGAAGAGT TGGGGCGCAN AATCGATACG CGCAACAATG TGCCCGATGC 1101 CGGCAAAANC AGCGTCCTCC GTATGGCGGA AGACGGCGCG ATTAAGCGCG 1151 CGCTGGTGCT GTTCCGAAAC AGCCGAACCG CCGGCGATGC GAAAATGCGC 1201 CGTCNGGCTC AGGCGGAATG GCGTTTCGCC ACACGCGGCT TCGATGAAGA 1251 CAAGCTGCTG ACCGCCGCGC AAACCGCGTT CGACCACGGT TTTTACGATA 1301 TGGCGGTCAA CAGCGCGGAA CGCACCGACC GCAAACTCAA CTACACCTTG 1351 CGCTACATTT CGNNNNNTNA ΝGACACGGTA ATCCGCCACG CGCAAAATGT 1401 TAATGTCGAT CCGGCGTGGG TTTACGGGCT GATTCGTCAG GAAAGCCGCT 1451 TCGTTATGGG CGCGCAATCC CGCGTAGGCG CGCAGGGGCT GATGCAGGTT 1501 ATGCCTGCCA CCGCGCGCGA AATCGCCGGC AAAATCGGTA TGGATGCCGC 1551 ACAACTTTAC ACCGCCGACG GCAATATCCG TATGGGGACG TGGTATATGG 1601 CGGACACCAA ACGCCGCCTG CAAAACAACG AAGTCCTCGC CACCGCAGGC 1651 TATAACGCCG GTCCCGGCAG GGCGCGCCGA TGGCAGGCGG ACACGCCCCT 1701 CGAAGGCGCG GTATATGCCG AAACCATCCC GTTTTCCGAA ACGCGCGACT 1751 ATGTCAAAAA AGTGATGGCC AATGCCGCCT ACTACGCCTC CCTCTTCGGC 1801 GCGCCGCACA TCCCGCTCAA ACAGCGTATG GGCATTGTCC CCGCCCGCTG 1851 A This corresponds to the amino acid sequence <SEQ ID 110; ORF 019.a> a019.pep MYPPSLKHSL PLLVXLVLAA CSXTΝTLSAD KTPADΝIETA DLSASVPTXP 51 AEPEXKTXAD YGGYPSALDA VKQKNDAAVA AYLENAGDSA MAENVRNEWL 101 KSLGARRQWT LXAXEYAKLE PAXRAQEVEC YADSSRNDYT RAAELVKNTG 151 KLPSGCTKLL EQAAASGLLD GNDAWRRVRG LLAGRQTTDA RNLAAALGSP 201 FDGGTQGSRE YALLNVIGKE ARKSPNAAAL LSEMESGLSL EQRSFAWGVL 251 GHYQSQΝLΝV PAALDYXGKV ADRRQLTDDQ IEWYARAAXX XRXXXXXAXX 301 XXXXXXKXXX XXXXXXXXAR SRAATGNTQX AXKLYKQAAA XGXNFYAVLX 351 GEELGRXIDT RNNVPDAGKX SVLRMAEDGA IKRALVLFRN SRTAGDAKMR 401 RXAQAEWRFA TRGFDEDKLL TAAQTAFDHG FYDMAVNSAE RTDRKLNYTL 451 RYISXXXDTV IRHAQNVNVD PAWVYGLIRQ ESRFVMGAQS RVGAQGLMQV 501 MPATAREIAG KIGMDAAQLY TADGNIRMGT WYMADTKRRL QNNEVLATAG 551 YNAGPGRARR WQADTPLEGA VYAETIPFSE TRDYVKKVMA NAAYYASLFG 601 APHIPLKQRM GIVPAR* mθi9/aθi9 88.9%) identity over a 524 aa overlap 10 20 30 40 50 60 m019.pep MYLPSMKHSLPLLAALVLAACSSTNTLPAGKTPADNIETADLSASVPTRPAEPERKTLAD II I lllllll: lllllll I I I I I I I I I I I I I I I I I I I I I I I I I I I I II II a019 MYPPSLKHSLPLLVXLVLAACSXTNTLSADKTPADNIETADLSASVPTXPAEPEXKTXAD 10 20 30 40 50 60 70 80 90 100 110 120 mOl 9. pep YGGYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKSLGARRQWTLFAQEYAKLE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I llllll aOl9 YGGYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKSLGARRQWTLXAXEYAKLE 70 80 90 100 110 120 130 140 150 160 170 180 m019.pep PAGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLLEQAAASGLLDGNDAWRRVRG II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I a019 PAXRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLLEQAAASGLLDGNDAWRRVRG 130 140 150 160 170 180 190 200 210 220 230 240 m019.pep LLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIGKEARKSPNAAALLSEMESGLSL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I a019 LLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIGKEARKSPNAAALLSEMESGLSL 190 200 210 220 230 240 250 260 270 280 290 300 mOl 9. pep EQRSFAWGVLGHYQSQNLNVPAALDYYGKVADRRQLTDDQIEWYARAALRARRWDELASV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I aOl 9 EQRSFAWGVLGHYQSQNLNVPAALDYXGKVADRRQLTDDQIEWYARAAXXXRXXXXXAXX 250 260 270 280 290 300 310 320 330 340 350 360 mOl 9. pep ISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKLYKQAAATGRNFYAVLAGEELGRKIDT I : I I I I I I I I I I I I I I I I I I I I : I llllll llllll III a019 XXXXXXKXXXXXXXXXXXARSRAATGNTQXAXKLYKQAAAXGXNFYAVLXGEELGRXIDT 310 320 330 340 350 360 370 380 390 400 410 420 mO19. pep RNNVPDAGKNSVRRMAEDGAVKRALVLFQNSQSAGDAKMRRQAQAEWRFATRGFDEDKLL I I I I I I I I I II I I I I I I I : I I I I I I I : I I :: I I I I I I I I I I I I I I I I I I I I I I I I I I aOl 9 RNNVPDAGKXSVLRMAEDGAIKRALVLFRNSRTAGDAKMRRXAQAEWRFATRGFDEDKLL 370 380 390 400 410 420 430 440 450 460 470 480 mOl 9. pep TAAQTAFDHGFYDMAVNSAERTDRKLNYTLRYISPFKDTVIRHAQNVNVDPAWVYGLIRQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I II aOl 9 TAAQTAFDHGFYDMAVNSAERTDRKLNYTLRYISXXXDTVIRHAQNVNVDPAWVYGLIRQ 430 440 450 460 470 480 490 500 510 520 m019.pep ESRFVIGAQSRVGAQGLMQVMPATAREIAGKIGMDAAQLYTADG I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a019 ESRFVMGAQSRVGAQGLMQVMPATAREIAGKIGMDAAQLYTADGNIRMGTWYMADTKRRL 490 500 510 520 530 540 aOl 9 QNNEVLATAGYNAGPGRARRWQADTPLEGAVYAETIPFSETRDYVKKVMANAAYYASLFG 550 560 570 580 590 600 Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 019 shows 95.5% identity over a 89 aa overlap with a predicted ORF (ORF 019.ng) from N gonorrhoeae: g019/m019 10 20 30 40 49 g019.pep LLAALVLAACSSTΝTLPAGKTPADΝIETADLSASVPTRPAEPEGKTLAD M i l l m019 MYLPSMKHSLPLLAALVLAACSSTΝTLPAGKTPADΝIETADLSASVPTRPAEPERKTLAD 10 20 30 40 50 60 50 60 70 80 89 gO 19 . pep YGGYPSALDAVKQΝΝDAAAAAYLEΝAGDSAMAEΝVRKEWL m019 YGGYPSALDAVK Q KΝDAAVAAYLEΝAGDSAMAEΝVRΝEWLKSLGARRQWTLFAQEYAKLE 70 80 90 100 110 120 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 111>: g023.seq 1 ATGGTAGAAC GTAAATTGAC CGGTGCCCAT TACGGTTTGC GCGATTGGGT 51 AATGCAGCGT GCGACTGCGG TTATTATGTT GATTTATACC GTTGCACTTT 101 TAGTGGTTCT ATTTGCCCTG CCTAAAGAAT ATCCGGCATG GCAGGCATTT 151 TTTAGTCAAG CTTGGGTAAA AGTATTTACC CAAGTGAGCT TTATCGCCGT 201 ATTCTTGCAC GCTTGGGTGG GTATCCGCGA TTTGTGGATG GACTATATCA 251 AACCCTTCGG CGTGCGTTTG TTTTTGCAGG TTGCCACCAT TGtctGGCTG 301 GTCGGCTGCC TCGTGTATTC AGTTAAAGTG ATTTGGGGGT AA This corresponds to the amino acid sequence <SEQ ID 112; ORF 023.ng>: g023.pep 1 MVERKLTGAH YGLRDWVMQR ATAVIMLIYT VALLWLFAL PKEYPAWQAF 51 FSQAWVKVFT QVSFIAVFLH AWVGIRDLWM DYIKPFGVRL FLQVATIVWL 101 VGCLVYSVKV IWG* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 113>: m023.seq 1 ATGGTAGAAC GTAAATTGAC CGGTGCCCAT TACGGTTTGC GCGATTGGGT 51 GATGCAACGT GCGACTGCGG TTATTATGTT GATTTATACC GTTGCACTTT 101 TAGTGGTTCT ATTTTCCCTG CCTAAAGAAT ATTCGGCATG GCAGGCATTT 151 TTTAGTCAAA CTTGGGTAAA AGTATTTACC CAAGTGAGCT TCATCGCCGT 201 ATTCTTGCAC GCTTGGGTGG GTATCCGCGA TTTGTGGATG GACTATATCA 251 AACCCTTCGG CGTGCGTTTG TTTTTGCAGG TTGCCACCAT CGTTTGGCTG 301 GTCGGCTGTC TCGTGTATTC AGTTAAAGTG ATTTGGGGGT AA This corresponds to the amino acid sequence <SEQ ID 114; ORF 023>: m023.pep 1 MVERKLTGAH YGLRDWVMQR ATAVIMLIYT VALLWLFSL PKEYSAWQAF 51 FSQTWVKVFT QVSFIAVFLH AWVGIRDLWM DYIKPFGVRL FLQVATIVWL 101 VGCLVYSVKV IWG* The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 115>: a023. seq 1 ATGGTAGAAC GTAAATTGAC CGGTGCCCAT TACGGTTTGC GGGATTGGGC 51 GATGCAACGT GCGACCGCGG TTATTATGTT GATTTATACC GTTGCACTTT 101 TAGTGGTTCT ATTTGCTCTG CCTAAAGAAT ATTCGGCATG GCAGGCATTT 151 TTTAGTCAAA CTTGGGTAAA AGTATTTACC CAAGTGAGCT TCATCGCCGT 201 ATTCTTGCAC GCTTGGGTGG GTATCCGCGA TTTGTGGATG GACTATATNA 251 AACCCTTCGG CGTGCGTTTG TTTTTGCAGG TTGCCACCAT CGTCTGGCTG 301 GTCGGCTGCT TGGTGTATTC AATTAAAGTA ATTTGGGGGT AA This corresponds to the amino acid sequence <SEQ ID 116; ORF 023. a>: a023.pep 1 MVERKLTGAH YGLRDWAMQR ATAVIMLIYT VALL LFAL PKEYSAWQAF 51 FSQTWVKVFT QVSFIAVFLH AWVGIRDLWM DYXKPFGVRL FLQVATIVWL 101 VGCLVYSIKV IWG* m023/a023 96.5%o identity over a 113 aa overlap 10 20 30 40 50 60 m023. pep MVERKLTGAHYGLRDWVMQRATAVIMLIYTVALLWLFSLPKEYSAWQAFFSQTWVKVFT I I I I I I I I I I I I I I I I : I I I I II I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I a023 MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLWLFALPKEYSAWQAFFSQTWVKVFT 10 20 30 40 50 60 70 80 90 100 110 m023.pep QVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVGCLVYSVKVIWGX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I II I I I a023 QVSFIAVFLHAWVGIRDLWMDYXKPFGVRLFLQVATIVWLVGCLVYSIKVIWGX 70 80 90 100 110 Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 023 shows 97.3% identity over a 113 aa overlap with a predicted ORF (ORF 023.ng) from N gonorrhoeae: g023/m023 10 20 30 40 50 60 g023.pep MVERKLTGAHYGLRDWVMQRATAVIMLIYTVALLWLFALPKEYPAWQAFFSQAWVKVFT 1 1 1 1 1 1 1 1 : 1 1 1 1 1 1 m023 MVERKLTGAHYGLRDWVMQRATAVIMLIYTVALLWLFSLPKEYSAWQAFFSQTWVKVFT 10 20 30 40 50 60 70 80 90 100 110 g023.pep QVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVGCLVYSVKVIWGX m023 QVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVGCLVYSVKVIWGX 70 80 90 100 110 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 117>: g025.seq 1 ATGTTGAAAC AAAcgACACT TTTGGCAGCT TGTACCGCCG TTGCCGCTCT 51 GTTGGGCGGT TGcgCCACCC AACAGCCTGC TccTGTCATT GCAGGCAATT 101 CAGGTATGCA GACCGTATCG TCTGCGCCGG TTTACAATCC TTATGGCGCA 151 ACGCCGTACA ATGCCGCTCC TGCCGCCAac gatgcGCCgT ATGTGCCGCC 201 CGTGCAAact gcgccggttT ATTCGCCTCC TGCTTATGTT CCGCcgtCTG 251 CACCTGCCGT TTCGGgtaca tatgtTCCTT CTTACGCACC CgtcgACATC 301 aacgCGGCGa cgCataCTAT TGTGCGTGGC GACACgGtgt acaACATTTc 351 caaAcgCtac CATATCTCTC AAGACGATTT CCGTGCGTGG AACGGCATGA 401 CCGACAATAC GTTGAGCATC GGTCAGATTG TTAAAGTCAA ACCGGCaggA 451 TATGCCGCAC CGAAAACCGC AGCCGTAGAA AGCAGGCCCG CCGTACCGGC 501 TGCCGCGCAA ACCCCTGTGA AACCCGCCGC gcaACCGCCC GTTCAGTCCG 551 CGCCGCAACC TGCCGCGCCC GCTGCGGAAA ATAAAGCGGT TCCCGCCCCC 601 GCGCCCGCCC CGCAATCTCC TGCCGCTTCG CCTTCCGGCA CGCGTTCGGT 651 CGGCGGCATT GTTTGGCAGC GTCCGACCCA AGGTAAAGTG GTTGCCGATT 701 TCGGCGGCGG CAACAAGGGT GTCGATATTG CCGGCAATGC CGGACAACCC 751 GTTTTGGCGG CGGCTGACGG CAAAGTGGTT TATGCCGGTT CAGGTTTGAG 801 GGGATACGGA AACTTGGTCA TCATCCAGCA CAATTCCTCT TTCCTGACCG 851 CGTACGGGCA CAACCAAAAA TTGCTGGTCG GCGAAGGTCA GCAGGTCAAA 901 CGCGGTCAGC AGGTTGCTTT GATGGGTAAT ACCGATGCTT CCAGAACGCA 951 GCTTCATTTC GAGGTGCGTC AAAACGGCAA ACCGGTTAAC CCGAACAGCT 1001 ATATCGCGTT CTGA This corresponds to the amino acid sequence <SEQ ID 118; ORF 025.ng>: g025.pep 1 MLKQTTLLAA CTAVAALLGG CATQQPAPVI AGNSGMQTVS SAPVYNPYGA 51 TPYNAAPAAN DAPYVPPVQT APVYSPPAYV PPSAPAVSGT YVPSYAPVDI 101 NAATHTIVRG DTVYNISKRY HISQDDFRAW NGMTDNTLSI GQIVKVKPAG 151 YAAPKTAAVE SRPAVPAAAQ TPVKPAAQPP VQSAPQPAAP AAENKAVPAP 201 APAPQSPAAS PSGTRSVGGI VWQRPTQGKV VADFGGGNKG VDIAGNAGQP 251 VLAAADGKW YAGSGLRGYG NLVIIQHNSS FLTAYGHNQK LLVGEGQQVK 301 RGQQVALMGN TDASRTQLHF EVRQNGKPVN PNSYIAF* The following partial DNA sequence was identified in N meningitidis <SEQ ID 119>: m025.seq (partial) 1 ..GTGCCGCCGG TGCAAAGCGC GCCGGTTTAT ACGCCTCCTG CTTATGTTCC 51 GCCGTCTGCA CCTGCCGTTT CGGGTACATA CGTTCCTTCT TACGCACCCG 101 TCGACATCAA CGCGGCGACG CATACTATTG TGCGCGGCGA CACGGTGTAC 151 AACATTTCCA AACGCTACCA TATCTCTCAA GACGATTTCC GTGCGTGGAA 201 CGGCATGACC GACAATACGT TGAGCATCGG TCAGATTGTT AAAGTCAAAC 251 CGGCAGGATA TGCCGCACCG AAAGCCGCAG CCGTAAAAAG CAGGCCCGCC 301 GTACCGGCTG CCGCGCAACC GCCCGTACAG TCCGCACCCG TCGACATTAA 351 CGCGGCGACG CATACTATTG TGCGCGGCGA CACGGTGTAC AACATTTCCA 401 AACGCTACCA TATCTCTCAA GACGATTTCC GTGCGTGGAA CGGCATGACC 451 GACAATATGT TGAGCATCGG TCAGATTGTT AAAGTCAAAC CGGCAGGATA 501 TGCCGCACCG AAAACCGCAG CCGTAGAAAG CAGGCCCGCC GTACCGGCTG 551 CCGTGCAAAC CCCTGTGAAA CCCGCCGCGC AACCGCCTGT GCAGTCCGCG 601 CCGCAACCTG CCGCGCCCGC TGCGGAAAAT AAAGCGGTTC CCGCGCCCGC 651 CCCGCAATCT CCTGCCGCTT CGCCTTCCGG CACGCGTTCG GTCGGCGGCA 701 TTGTTTGGCA GCGTCCGACG CAAGGTAAAG TGGTTGCCGA TTTCGGCGGC 751 AACAACAAGG GTGTCGATAT TGCCGGTAAT GCGGGACAGC CCGTTTTGGC 801 GGCGGCTGAC GGCAAAGTGG TTTATGCCGG TTCAGGTTTG AGGGGATACG 851 GAAACTTGGT CATCATCCAG CATAATTCTT CTTTCCTGAC CGCATACGGG 901 CACAACCAAA AATTGCTGGT CGGCGAGGGG CAGCAGGTCA AACGCGGTCA 951 GCAGGTTGCT TTGATGGGCA ATACCGATGC TTCCAGAACG CAGCTTCATT 1001 TCGAGGTGCG TCAAAACGGC AAACCGGTTA ACCCGAACAG CTATATCGCG 1051 TTCTGA This corresponds to the amino acid sequence <SEQ ID 110; ORF 025>: m025.pep (partial) 1 ..VPPVQSAPVY TPPAYVPPSA PAVSGTYVPS YAPVDIΝAAT HTIVRGDTVY 51 ΝISKRYHISQ DDFRAWΝGMT DΝTLSIGQIV KVKPAGYAAP KAAAVKSRPA 101 VPAAAQPPVQ SAPVDIΝAAT HTIVRGDTVY ΝISKRYHISQ DDFRAWΝGMT 151 DΝMLSIGQIV KVKPAGYAAP KTAAVESRPA VPAAVQTPVK PAAQPPVQSA 201 PQPAAPAAEΝ KAVPAPAPQS PAASPSGTRS VGGIVWQRPT QGKWADFGG 251 ΝΝKGVDIAGΝ AGQPVLAAAD GKWYAGSGL RGYGΝLVIIQ HΝSSFLTAYG 301 HΝQKLLVGEG QQVKRGQQVA LMGΝTDASRT QLHFEVRQΝG KPVΝPΝSYIA 351 F* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 111>: a025. seq 1 ATGTTGACAC CAACAACACT TTAGGTAGCT TGTACCGCCC TTGCCGCTCA 51 GTTGGGCGGA TGCCCCACCC AACACCCTTC TCCTGTCATT GCAGGCAATT 101 CAGGTATGCA GACCGTACCG TCTGCGCCGG TTTACAATCC TTATGGCGCA 151 ACGCCGTACA ATGCCGCTCC TGCCGCCAAC GATGCGCCGT ATGTGCCGCC 201 GGTGCAAAGC GCGCCGGTTT ATANGCCTCC TGCTTATGTT CCGCCGTCTG 251 CACCTGCCGT TTCGGGTACA TACGTTCCTT CTTACGCANC CGTCGACATC 301 AACGCGGCGA CCCATACTAT TGTGCGCGGC GACACCGTGT ACAAGATTTC 351 CAAATGCTAC CATATCTCTC AAGACGATTT CCGTGCGTGG AACGGCATGA 401 CCGACAATAC GTTGAGCATC GGTCAGATTG TTAAAGTCAA ACCGGCAGGA 451 TATGCCGCAC CGAAAGCCGC AGCCGTAAAA AGCAGGCCCG CCGTACCGGC 501 TGCCGCGCAA CCGCTCGTAC AGTCCGCACC CGTCGACATC AACGCGGCGA 551 CGCATACTAT TGTGCGCGGC GACACGGTGT ACAACATTTC CAAACGCTAC 601 CATATCTCTC AAGACGATTT CCGTGCGTGG AACGGCATGA CCGACAATAC 651 GTTGAGCATC GGTCAGATTG TTAAAGTCAA ACCGGCAGGA TATGCCGCAC 701 CGAAAGCCGC AGCCGTAAAA AGCAGGCCCG CCGTACCGGC TGCCGTGCAA 751 ACCCCTGTGA AACCCGCCGC GCAACCGCCT GTGCAGTCCG CGCCGCAACC 801 TGCCGCGCCC GCTGCGGAAA ATAAAGCGGT TCCCGCGCCC GCCCCGCAAT 851 CTCCTGCCGC TTCGCCTTCC GGCACGCGTT CGGTCGGCGG CATTGTTTGG 901 CAGCGTCCGA CGCAAGGTAA AGTGGTTGCC GATTTCGGCG GCAACAACAA 951 GGGTGTCGAT ATTGCAGGAA ATGCGGGACA GCCCGTTTTG GCGGCGGCTG 1001 ACGGCAAAGT GGTTTATGCA GGTTCCGGTT TGAGGGGATA CGGCAATTTG 1051 GTCATCATCC AGCATAATTC TTCCTTCCTG ACCGCATACG GGCACAACCA 1101 AAAATTGCTG GTCGGCGAAG GCCAGCAGGT CAAACGCGGG CAGCAGGTCG 1151 CTTTGATGGG CAATACCGAG GCTTCTAGAA CGCAGCTTCA TTTCGAGGTG 1201 CGGCAAAACG GCAAACCGGT TAATCCGAAC AGCTATATCG CGTTCTGA This corresponds to the amino acid sequence <SEQ ID 112; ORF 025. a>: a025.pep 1 MLTPTTL*VA CTALAAQLGG CPTQHPSPVI AGNSGMQTVP SAPVYNPYGA 51 TPYNAAPAAN DAPYVPPVQS APVYXPPAYV PPSAPAVSGT YVPSYAXVDI 101 NAATHTIVRG DTVYKISKCY HISQDDFRAW NGMTDNTLSI GQIVKVKPAG 151 YAAPKAAAVK SRPAVPAAAQ PLVQSAPVDI NAATHTIVRG DTVYNISKRY 201 HISQDDFRAW NGMTDNTLSI GQIVKVKPAG YAAPKAAAVK SRPAVPAAVQ 251 TPVKPAAQPP VQSAPQPAAP AAENKAVPAP APQSPAASPS GTRSVGGIVW 301 QRPTQGKWA DFGGNNKGVD IAGNAGQPVL AAADGKWYA GSGLRGYGNL 351 VIIQHNSSFL TAYGHNQKLL VGEGQQVKRG QQVALMGNTE ASRTQLHFEV 401 RQNGKPVNPN SYIAF* m025/a025 97.4% identity over a 351 aa overlap 10 20 30 m025. pep VPPVQSAPVYTPPAYVPPSAPAVSGTYVPS I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I a025 GMQTVPSAPVYNPYGATPYNAAPAANDAPYVPPVQSAPVYXPPAYVPPSAPAVSGTYVPS 40 50 60 70 80 90 40 50 60 70 80 90 m025.pep YAPVDINAATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNTLSIGQIVKVKPAGYAAP II I I I I I I I I I I I I I I I I I: I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I a025 YAXVDINAATHTIVRGDTVYKISKCYHISQDDFRAWNGMTDNTLSIGQIVKVKPAGYAAP 100 110 120 130 140 150 100 110 120 130 140 150 m025. pep KAAAVKSRPAVPAAAQPPVQSAPVDINAATHTIVRGDTVYNISKRYHISQDDFRAWNGMT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I a025 KAAAVKSRPAVPAAAQPLVQSAPVDINAATHTIVRGDTVYNISKRYHISQDDFRAWNGMT 160 170 180 190 200 210 160 170 180 190 200 210 m025. pep DNMLSIGQIVKVKPAGYAAPKTAAVESRPAVPAAVQTPVKPAAQPPVQSAPQPAAPAAEN II I I I I I I I I I I I I I I I I I I: I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a025 DNTLSIG Q IVKVKPAGYAAPKAAAVKSRPAVPAAVQTPVKPAAQPPVQSAPQPAAPAAEN 220 230 240 250 260 270 220 230 240 250 260 270 m025. pep KAVPAPAP Q SPAASPSGTRSVGGIVWQRPTQGKWADFGGNNKGVDIAGNAGQPVLAAAD I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I l l l a025 KAVPAPAPQSPAASPSGTRSVGGIVWQRPTQGKWADFGGNNKGVDIAGNAGQPVLAAAD 280 290 300 310 320 330 280 290 300 310 320 330 m025.pep GKWYAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDASRT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I a025 GKWYAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTEASRT 340 350 360 370 380 390 340 350 m025.pep QLHFEVRQNGKPVNPNSYIAFX I I I I I I I I I I I I I I I I I I I I I I a025 QLHFEVRQNGKPVNPNSYIAFX 400 410 Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 025 shows 75.6% identity over a 353 aa overlap with a predicted ORF (ORF 025. ng) from N gonorrhoeae: m025/g025 10 20 30 m025. ■Pep VPPVQSAPVYTPPAYVPPSAPAVSGTYVPS Mll lll lllMIIIMMIMIIM g025 GMQTVSSAPVYΝPYGATPYΝAAPAAΝDAPYVPPVQTAPVYSPPAYVPPSAPAVSGTYVPS 40 50 60 70 80 90 40 50 60 70 80 90 m025. .pep YAPVDIΝAATHTIVRGDTVYΝISKRYHISQDDFRAWΝGMTDΝTLSIGQIVKVKPAGYAAP g025 YAPVDIΝAATHTIVRGDTVYΝISKRYHISQDDFRAWΝGMTDΝTLSIGQIVKVKPAGYAAP 100 110 120 130 140 150 100 110 120 130 140 150 m025.pep KAAAVKSRPAVPAAAQPPVQSAPVDIΝAATHTIVRGDTVYΝISKRYHISQDDFRAWΝGMT g025 160 170 180 190 200 210 m025.pep DΝMLSIGQIVKVKPAGYAAPKTAAVESRPAVPAAVQTPVKPAAQPPVQSAPQPAAPAAEΝ g025 TAAVESRPAVPAAAQTPVKPAAQPPVQSAPQPAAPAAEΝ 160 170 180 190 220 230 240 250 260 m025. ■Pep KAVPAPAP--QSPAASPSGTRSVGGIVWQRPTQGKWADFGGΝΝKGVDIAGΝAGQPVLAA MINIM MMMMMIMIMIMMMMMMMhlMIMMMMIMM g025 KAVPAPAPAPQSPAASPSGTRSVGGIVWQRPTQGKWADFGGGNKGVDIAGNAGQPVLAA 200 210 220 230 240 250 270 280 290 300 310 320 m025 •Pep ADGKWYAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDAS MMMIMMMMMIMMMMMMIMIMMMMIMMIMMMMMM g025 ADGKWYAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDAS 260 270 280 290 300 310 330 340 350 m025. ■pep RTQLHFEVRQNGKPVNPNSYIAFX g025 RTQLHFEVRQNGKPVNPNSYIAFX 320 330 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 113>: g031.seq 1 ATGGTGTCCC TCCGCTTCAG ATTCGGCAAC CACTTTAAAC GCCGACATTC 51 TGACAATTTC CTTTTCCGCC AGCCAAATAT CATGCGTATC TTTCGGTTCG 101 GGCTTGTTGG GCATGGCAAC CTTCAACAGC CGCGCCATCA CAGGAATCGT 151 CGTTCCCTGA ATCAGCAGCG ACAGCACCAC CACGGCAAAC GCCACATCAA 201 ACAGCAGGTG CGAATTGGGA ACGCCCATCA CCAGCGGCAT CATCGCCAGC 251 GAAATCGGTA CGGCTCCTCG CAAGCCCAAC CAACTGATAT ACGCCTTTTC 301 ACGCAGGCTG TAATTGAATT TCCACAAACC GCCGAACACT GCCAGCGGAC 351 GCGCGACCAG CATCAGGAAC GCCGCAATCG CCAAGGCTTC CGCCGCCCTG 401 TCCAACACGC CGGCGGGAGA AACCAGCAGA CCGAGCATGA CGAACAAAGT 451 TGCCTGCGCC AGCCAAGCCA AACCGTCCAT CACACGCAAA ACGTGTTCCG 501 TcgcACGGTT GCGCTGGTTA CCGACAATGA TGCCGGCAAG GTAAACCGCC 551 AAAAAGCCGC TGCCGCCTAT GGTATTGGTA AACGCAAACA CAAGCAGCCC 601 GCCCGACACA ATCATCAGCG CGTACAGACC TTCCGtacac acctccaatt 651 cccaatcaac gtcatagctg tctcccgtgt taaaatgttc ttcacttcag 701 aatccccccc ttcttcccag cccgaaacct tcatgtgtta naccctgggg 751 tgccccaacg gatttagtaa cctcccaatg actctgcttg tcgccccctt 801 cgcccgcttt ctccttccgg gaaaacttgt tgtccccgtc ttacattaa This corresponds to the amino acid sequence <SEQ ID 114; ORF 031.ng>: g031.pep 1 MVSLRFRFGΝ HFKRRHSDΝF LFRQPΝIMRI FRFGLVGHGΝ LQQPRHHRΝR 51 RSLΝQQRQHH HGKRHIKQQV RIGΝAHHQRH HRQRΝRYGSS QAQPTDIRLF 101 TQAVIEFPQT AEHCQRTRDQ HQERRΝRQGF RRPVQHAGGR ΝQQTEHDEQS 151 CLRQPSQTVH HTQΝVFRRTV ALVTDΝDAGK VΝRQKAAAAY GIGKRKHKQP 201 ARHΝHQRVQT FRTHLQFPIΝ VIAVSRVKMF FTSESPPSSQ PETFMCXTLG 251 CPΝGFSΝLPM TLLVAPFARF LLPGKLWPV LH* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 115>: m031.seq (partial) 1 ... CGCCTGAAGC ACGGTGTCGG ACTGCATTTC TATTCGGCTA TACGCCTTTT 51 CACGCAGGCT GTAATTGAAT TTCCACAAAC CGCCGAACAC TGCCGACGGA 101 CGCGCGACCA GCATCAGGAA CGCCGCAATC GCCAAgGCTT CCGCCGCCCT 151 GTCCAACACG TTGGCAGGAG AAACCAGCAG CAAAGGCATT CCCAAACGTG 201 CGGACAAAGT GGTCGAAACC ACGCTCAGAA ACAACAGTGC GCCACCCGGC 251 AG This corresponds to the amino acid sequence <SEQ ID 116; ORF 031>: m031.pep (partial) 1 ... RLKHGVGLHF YSAIRLFTQA VIEFPQTAEH CRRTRDQHQE RRΝRQGFRRP 51 VQHVGRRΝQQ QRHSQTCGQS GRΝHAQKQQC ATRQ.... The following partial DΝA sequence was identified in N meningitidis <SEQ ID 117>: a031. seq 1 ATACGCCTTT TCACGCAGGC TGTAATTGAA TTTCCACAAA CCGCCGAACA 51 CTGCCGGCGG ACGCGCGACC AGCATCAGGA ACGCCGCAAT CGCCAAGGCT 101 TCCGCCGCCC CGTCCAACAC GTTGGCAGGA GAAACCAGCA GCAAAGGCAT 151 TCCCAAACGT GCGGACAAAG TGGTCGAAAC CACGCTCAGA AACAACAGTG 201 CGCCACCCGG CAG This corresponds to the amino acid sequence <SEQ ID 118; ORF 031.a>: a031.pep (partial) 1 IRLFTQAVIE FPQTAEHCRR TRDQHQERRΝ RQGFRRPVQH VGRRΝQQQRH 51 SQTCGQSGRΝ HAQKQQCATR Q m03i/a03i 100.0% identity over a 71 aa overlap 10 20 30 40 50 60 m031. pep RLKHGVGLHFYSAIRLFTQAVIEFPQTAEHCRRTRDQHQERRNRQGFRRPVQHVGRRNQQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M I I I I I a031 IRLFTQAVIEFPQTAEHCRRTRDQHQERRNRQGFRRPVQHVGRRNQQ 10 20 30 40 70 80 mO31. pep QRHSQTCGQSGRNHAQKQQCATRQ I I I I I I I I I I I I I I I I I I I I I I I I a031 QRHSQTCGQSGRNHAQKQQCATRQ 50 60 70 Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 031 shows 60.0% identity over a 85 aa overlap with a predicted ORF (ORF 031.ng) from N. gonorrhoeae: m031/g031 10 20 30 m031.pep RLKHGVGLHFYSAIRLFTQAVIEFPQTAEH I : : I : : I I I I I I I I I I I I I I I I I g031 ΝQQRQHHHGKRHIKQQVRIGΝAHHQRHHRQRΝRYGSSQAQPTDIRLFTQAVIEFPQTAEH 60 70 80 90 100 110 40 50 60 70 80 m031.pep CRRTRDQHQERRΝRQGFRRPVQHVGRRΝQQQRHS-QTCGQSGRΝHAQKQQCATRQ I : I I I I I I I I I I I I I I I I I I I I I : I I I I I : I : I : I : : : : : : I : I : g031 CQRTRDQHQERRΝRQGFRRPVQHAGGRΝQQTEHDEQSCLRQPSQTVHHTQΝVFRRTVALV 120 130 140 150 160 170 g031 TDΝDAGKVΝRQKAAAAYGIGKRKHKQPARHΝHQRVQTFRTHLQFPIΝVIAVSRVKMFFTS 180 190 200 210 220 230 The following partial DΝA sequence was identified in N. gonorrhoeae <SEQ ID 119>: g032. seq 1 ATGCGGCGAA ACGTGCCTGC CGTCGCCGTA TTGCGCCGCC CACGATTCGA 51 GGCGTTTTTG GATTTGGCGT TGGCTCAGGC GCGTGCCGTT CCTGCCGGTA 101 AACAGGGCTT TGCCGTCCGA TGCCGTCTGA CGCAGCGGCA GATAGTTTTT 151 CAAGGCTTCC ACGCTTTTGC CGGTCAGCGG AACCTGACGC TGCTTGCGCC 201 CTTTGCCGGT AACGTGTACC CACGCTTCGT CCAAATATAC ATCATCTGCA 251 TTCAAGCCGT GTATCTCGCT CACGCGCAAA CCGCTGCCGT ACATCAGCTC 301 GAACAGCGCG TGGTCGCGCA CCGCCAGCGG GTCGCCGCCG TCCACGGGCA 351 AATCCAACAT CCGGTTCAGC CATTCCTGCG GCAGGGCTTT GGGTACGCGC 401 TCGGGCTGCT TCGGCGGTTT GATGTCGGCG GTCGGGTCGG CGCGCATCAG 451 CCCGCGTTTG ACCAGCCAGG CGCAATACTG CCGCCACGCC GACAGCTTGC 501 GCGCCAGCGT CCGACCGTCC AAACCGCGCT GCGACAGCCG CCGCAACGCC 551 GccgTAAAAT CGCGCCGCGA CAAGTCCTGC GGCACGCcgc ctgcaTCTTC 601 AGACGGCATT TGTGCCAACA GTGCAAACAG TTCTTCCAAA TCGCGCCGGT 651 ATGCCGCAAC CGTGTGCTCC GACTTGCCCT CGCGCACGAT GTTTTCCAAA 701 TAAGCGTCAA AATacgccgC AAACccgTCC AAAACCATAA CCGTCCCACA 751 CAAATATCAA AAAACCAGTG A This corresponds to the amino acid sequence <SEQ ID 110; ORF 032.ng>: g032.pep 1 MRRΝVPAVAV LRRPRFEAFL DLALAQARAV PAGKQGFAVR CRLTQRQIVF 51 QGFHAFAGQR ΝLTLLAPFAG ΝVYPRFVQIY IICIQAVYLA HAQTAAVHQL 101 EQRWAHRQR VAAVHGQIQH PVQPFLRQGF GYALGLLRRF DVGGRVGAHQ 151 PAFDQPGAIL PPRRQLARQR PTVQTALRQP PQRRRKIAPR QVLRHAACIF 201 RRHLCQQCKQ FFQIAPVCRN RVLRLALAHD VFQISVKIRR KPVQNHNRPT 251 QISKNQ* The following partial DNA sequence was identified in N meningitidis <SEQ ID 111>: m032.seq (partial) 1 ATGCGGCGAA ACGTGCmTGC mGTCGCCGTT kTGCGCCGCC CATTGCGCCA 51 AACGTTTTTG GATTTGGCGT TGGCTCAGGC GCGTGCCGTT CCTGCCGGTA 101 AACAGGGCTT TGCCGTCCGA TGCCGTCTGA CGCAGCGGCA GATAGTTTTT 151 CAGGGCTTCC ACGCTTTTGC CGACCAGCGG CACCTGCCGC TgTT . GCGCC 201 CTTTGCCGAT AAcGTGTACC CACGCyTCGT CCAAATAGAC ATCATCTGCA 251 TTCAAGCCGT GTATCTCGCT CACGCGCAAA CCGCTGCCGT ACATCAGTTC 301 GAACAGGGCG TGGTCGCGCA CCGCCAGCGG GTCGCCGCCG TCCACGGGCA 351 AATCCAGCAT CCGGTTCAGC CATTCCTGCG GCAGGGCTTT GGGTACGCGC 401 TCGGGCTGCT TCGGCGGTTT GATGTCGGCG GTCGGGTCGG CGTGCATCAG 451 GCCGCGCTTT ACCAGCCAAA CGCAATACTG CCGCCAAGAC GAAAGCTTGC 501 GAGCCAGCGT CCGTTCCCCC AAACCGCG... This corresponds to the amino acid sequence <SEQ ID 112; ORF 032>: m032.pep (partial) 1 MRRΝVXAVAV XRRPLRQTFL DLALAQARAV PAGKQGFAVR CRLTQRQIVF 51 QGFHAFADQR HLPLXAPFAD ΝVYPRXVQID IICIQAVYLA HAQTAAVHQF 101 EQGWAHRQR VAAVHGQIQH PVQPFLRQGF GYALGLLRRF DVGGRVGVHQ 151 AALYQPΝAIL PPRRKLASQR PFPQTA... The following partial DΝA sequence was identified in N meningitidis <SEQ ID 113>: a032. seq 1 ATGCGGCGAA ACGTGCCTGC CGTCGCCGTT TTGCGCCGCC CATTGCGCCA 51 AACGTTTTTG GATTTGGCGT TGGCTCAGGC GCGTGCCGTT CCTGCCGGTA 101 AACAGGGCTT TGCCGTCCGA TGCCGTCTGA CGCAGCGGCA GATAGTTTTT 151 CAGGGCTTCC ACGCTTTTGC CGGTCAGCGG AACCTGCCGC TGCTTGCGTC 201 CTTTGCCGGT AACGTGTACC CACGCCTCGT CCAAATATAC ATCATCTGCA 251 TTCAAGCCGT GTATCTCGCT CACGCGCAAA CCGCTGCCGT ACATCAGTTC 301 GAACAGCGCG TGATCGCGCA CCGCCAGCGG GTCGCCGCCG TCCACGGGCA 351 AATCCAGCAT CCGGTTCAGC CATTCCTGCG GCAGGGCTTT GGGTACGCGC 401 TCGGGCTGCT TCGGCGGTTT GATGTCGGCG GTCGGGTCGG TATGCAGCAG 451 ACCGCGTTTG ACCAGCCAGG CGCAATACTG CCGCCAAGAC GACAGCTTGC 501 GCGCCAGCGT CCGCGCATTC AAACCGCGCT GCGACAGCCG CCGCAACGCC 551 GCCGTAAAAT CGCGCTGCGA CAAGCCCTGC GGCACGCCGC CTGCATCTTC 601 AGACGGCATT TGTGCCAACA GCGCAAACAG TTCTTCCAAA TCGCGCCGGT 651 ATGCCGCCAC CGTGTGCTCC GACTTGCCCT CGCGCACGAT GTTTTCCAAA 701 TAAGCGTCAA AATGCGCCGC AAACCCGTCC AAAACCATAA CCGCCCCACA 751 CAAATATCAA AAAAACAGTG A This corresponds to the amino acid sequence <SEQ ID 114; ORF 032. a>: a032.pep 1 MRRΝVPAVAV LRRPLRQTFL DLALAQARAV PAGKQGFAVR CRLTQRQIVF 51 QGFHAFAGQR ΝLPLLASFAG ΝVYPRLVQIY IICIQAVYLA HAQTAAVHQF 101 EQRVIAHRQR VAAVHGQIQH PVQPFLRQGF GYALGLLRRF DVGGRVGMQQ 151 TAFDQPGAIL PPRRQLARQR PRIQTALRQP PQRRRKIALR QALRHAACIF 201 RRHLCQQRKQ FFQIAPVCRH RVLRLALAHD VFQISVKMRR KPVQΝHΝRPT 251 QISKKQ* 032/a032 88.1 % identity over a 176 aa overlap 10 20 30 40 50 60 m032. pep MRRΝVXAVAVXRRPLRQTFLDLALAQARAVPAGKQGFAVRCRLTQRQIVFQGFHAFADQR M I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II a032 MRRΝVPAVAVLRRPLR Q TFLDLALAQARAVPAGKQGFAVRCRLTQRQIVFQGFHAFAGQR 10 20 30 40 50 60 70 80 90 100 110 120 m032.pep HLPLXAPFADNVYPRXVQIDIICIQAVYLAHAQTAAVHQFEQGWAHRQRVAAVHGQIQH : I I I I II I I I I I III I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I a032 NLPLLASFAGNVYPRLVQIYIICIQAVYLAHAQTAAVHQFEQRVIAHRQRVAAVHGQIQH 70 80 90 100 110 120 130 140 150 160 170 m032.pep PVQPFLRQGFGYALGLLRRFDVGGRVGVHQAALYQPNAILPPRRKLASQRPFPQTA I I I I I I I I I I I I I I I I I I I I I I I I I I I :: I : I : I I : I I I I I I I : I I III III a032 PVQPFLRQGFGYALGLLRRFDVGGRVGMQQTAFDQPGAILPPRRQLARQRPRIQTALRQP 130 140 150 160 170 180 aO32 PQRRRKIALRQALRHAACIFRRHLCQQRKQFFQIAPVCRHRVLRLALAHDVFQISVKMRR 190 200 210 220 230 240 Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N. gonorrhoeae ORF 032 shows 86.4% identity over a 176 aa overlap with a predicted ORF (ORF 032.ng) from N gonorrhoeae: m032/g032 10 20 30 40 50 60 mO32. pep MRRΝVXAVAVXRRPLRQTFLDLALAQARAVPAGKQGFAVRCRLTQRQIVFQGFHAFADQR I I I I I I I I I III :: I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II g032 MRRΝVPAVAVLRRPRFEAFLDLALAQARAVPAGKQGFAVRCRLTQRQIVFQGFHAFAGQR 10 20 30 40 50 60 70 80 90 100 110 120 m032. pep HLPLXAPFADΝVYPRXVQIDIICIQAVYLAHAQTAAVHQFEQGWAHRQRVAAVHGQIQH : I I I I I I I I I I I III I I I I I I I I I I I I I I I I I II : I I I I I I I I I I I I I I I I I I I g032 ΝLTLLAPFAGΝVYPRFVQIYIICIQAVYLAHAQTAAVHQLEQRVVAHRQRVAAVHGQIQH 70 80 90 100 110 120 130 140 150 160 170 m032. pep PVQPFLRQGFGYALGLLRRFDVGGRVGVHQAALYQPΝAILPPRRKLASQRPFPQTA I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I : 11:1111111:11 III III g032 PVQPFLRQGFGYALGLLRRFDVGGRVGAHQPAFDQPGAILPPRRQLARQRPTVQTALRQP 130 140 150 160 170 180 g032 PQRRRKIAPRQVLRHAACIFRRHLCQQCKQFFQIAPVCRΝRVLRLALAHDVFQISVKIRR 190 200 210 220 230 240 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 115>: g033.seq 1 ATGGCGGCGG CGGACAAACT CTTGGGCGGC GACCGCCGCA GCGTCGCCAT 51 CATCGGAGAC GGCGCGATGA CGGCGGGGCA GGCGTTTGAA GCCTTGAATT 101 GCGCGGGCGA TATGGATGTG GATTTGCTGG TCGTCCTCAA CGACAACGAA 151 ATGTCGATTT CCCCCAACGT CGGCGCGTTG CCCAAATATC TTGCCAGCAA 201 CGTCGTGCGC GATATGCACG GACTGTTGAG TACCGTCAAA GCGCAAAcgg 251 GCAAGGTATT AGACAAAATA CCCGGCGCGA TGGagtTTGC CCAAAAAGTC 301 GAACAcaaaA TCAAAACCCT TGCCGAAGAA GCCGAACACG CCAAACAGTC 351 GCTGTCGCTG TTTGAAAATT TCGGCTTCCG CTACACCGGC CCCGTGGACG 401 GACACAACGT CGAGAATCTG GTGGACGTAT TGAAAGACTT GCGCAGCCGC 451 AAAGGCCCTC AGTTGCTGCA CGTCATCACC AAAAAGGGCA ACGGCTACAA 501 ACTCGCCGAA AACGACCCcg tcaAATACCA CGCCGTCGCC aACCTGCcta 551 AAGAAGGCGG GGCGCAAATg ccGTCTGAAA AAGAACCCAA GCCCGCCgCc 601 aaaccgACCT ATACCCAAGT ATTCGGCAAA TGGCTGTGCG ACCGGGCGGC 651 GGCAGATTCC CGACTGGTTG CGATTACCCC CGCCATGCGC GAGGGCAGCG 701 GACTGGTGGA GTTTGAACAA CGATTCCCCG ACCGCTATTT CGATGTCGGC 751 ATCGCCGAGC AGCACGCCGT tacCTTTGCC GGCGGTTTGG CGTGCGAAGG 801 CATGAAGCCC GTCGTGGCGA TTTATTCCAC CTTTTTACAA CGCGCCTACG 851 ACCAACTGGT GCACGACATC GCCCTGCAAA ACCTGCCCGT TTTGTTTGCC 901 GTCGACCGTG CGGGCATCGT CGGCGCGGAC GGTCCGACCC ATGCCGGCTT 951 GTACGATTTG AGCTTCTTGC GCTGTGTGCC GAACATGATT GTTGCCGCGC 1001 CGAGCGATGA AAACGAATGC CGCCTGCTGC TTTCGACCTG CTATCAGGCG 1051 GATGCGCCCG CCGCCGTCCG CTATCCGCGC GGCACGGGTA CGGGCGCGCC 1101 GGTTTCAGAC GGCATGGAAA CCGTGGAAAT CGGCAAGGGC ATTATCCGCC 1151 GCGAAGGTGA GAAAACCGCC TTcatTGCCT TCGGCAGTAT GGTCGCCACC 1201 GCATTGGCGG TTGCCGAAAA ACTGAACGCC ACCGTCGCCG ATATGCGCTt 1251 cgtcaaacCG ATAGACGAAG AGTTGATTGT CCGCCTTGCC CGAAGCCAcg 1301 accGCATCGT TACCCTTGAA GAAAACGCCG AACAGGGCGG CGCAGGCGGC 1351 GCGGTCTTGG AAGTGTTGGC GAAACACGGC ATCTGCAAAC CCGTTTTGCT 1401 TTTGGGCGTT GCCGATACCG TAACCGAACA CGGCGATCCG AAAAAACTTT 1451 TGGACGATTT GGGTTTGAGT GCCGAAGCGG TGGAACGCCG GGTGCGCGAG 1501 TGGCTGCCGG ACCGTGATGC GGCAAATTAA This corresponds to the amino acid sequence <SEQ ID 116; ORF 033.ng>: g033.pep 1 MAAADKLLGG DRRSVAIIGD GAMTAGQAFE ALNCAGDMDV DLLWLNDNE 51 MSISPNVGAL PKYLASNWR DMHGLLSTVK AQTGKVLDKI PGAMEFAQKV 101 EHKIKTLAEE AEHAKQSLSL FENFGFRYTG PVDGHNVENL VDVLKDLRSR 151 KGPQLLHVIT KKGNGYKLAE NDPVKYHAVA NLPKEGGAQM PSEKEPKPAA 201 KPTYTQVFGK WLCDRAAADS RLVAITPAMR EGSGLVEFEQ RFPDRYFDVG 251 IAEQHAVTFA GGLACEGMKP WAIYSTFLQ RAYDQLVHDI ALQNLPVLFA 301 VDRAGIVGAD GPTHAGLYDL SFLRCVPNMI VAAPSDENEC RLLLSTCYQA 351 DAPAAVRYPR GTGTGAPVSD GMETVEIGKG IIRREGEKTA FIAFGSMVAT 401 ALAVAEKLNA TVADMRFVKP IDEELIVRLA RSHDRIVTLE ENAEQGGAGG 451 AVLEVLAKHG ICKPVLLLGV ADTVTEHGDP KKLLDDLGLS AEAVERRVRE 501 WLPDRDAAN* The following partial DNA sequence was identified in N meningitidis <SEQ ID 117>: m033.seq 1 ATGGCGGCGG CAGACAAACT CTTGGGCAGC GACCGCCGCA GCGTCGCCAT 51 CATCGGCGAC GGCGCGATGA CGGCGGGGCA GGCGTTTGAA GCCTTGAATT 101 GCGCaG.CGA TATGGATGTr GATTTGCTrG TCGTCCTCAA CGACAACGAA 151 ATGTCGATTT CCCCCAACGT CGGCGCGCTG CCGAAATACC TTGCCAGCAA 201 CGTCGTGCGC GATATGCACG GCCTGTTGAG TACCGTCAAA GCGCAAACGG 251 GCAAGGTATT AGACAAAATA CCCGGCGCGA TGGAGTTTGC CCAAAAAGTC 301 GAACACAAAA TCAAAACCCT TGCCGAAGAA GCCGAACACG CCAAACAGTC 351 GCTGTCTTTG TTTGAAAACT TCGGCTTCCG CTACACCGGC CCCGTGGACG 401 GACACAACGT CGAAAATCTG GTGGACGTAT TGAAAGACTT GCGCAGCCGC 451 AAAGGCCCTC AGTTGCTGCA CGTCATCACC AAAAAGGGCA ACGGCTACAA 501 ACTCGCCGAA AACGACCCCG TCAAATACCA CGCCGTCGCC AACCTGCCTA 551 AAGAAAGCGC GGCGCAAATG CCGTCTGAAA AAGAACCCAA GCCCGCCGCC 601 AAACCGACCT ATACCCAAGT GTTCGGCAAA TGGCTGTGCG ACCGGGCGGC 651 GGCAGATTCC CGACTGGTTG CGATTACCCC CGCCATGCGC GAGGGCAGCG 701 GCTTGGTTGA GTTTGAACAA CGATTCCCCG ACCGCTATTT CGATGTCGGC 751 ATCGCCGAGC AGCACGCCGT TACCTTTGCC GGCGGTTTGG CTTGCGAAGG 801 GATGAAGCCC GTCGTGGCGA TTTATTCCAC CTTTTTACAA CGCGCCTACG 851 ACCAACTGGT GCACGACATC GCCCTGCAAA ACCTACCCGT TTTGTTTGCC 901 GTCGACCGCG CGGGCATCGT CGGCGCGGAC GGCCCGACCC ATGCCGGTCT 951 GTACGATTTG AGCTTTTTGC GCTGCGTGCC GAACATGATT GTCGCCGCGC 1001 CGAGCGATGA AAACGAATGC CGCCTGTTGC TTTCGACCTG CTATCAGGCA 1051 GACGCGCCCG CCGCCGTCCG CTATCCGCGC GGCACGGGTA CGGGCGCGCC 1101 GGTTTCAGAC GGCATGGAAA CCGTGGAAAT CGGCAAGGGC ATTATCCGCC 1151 GCGAAGGTGA GAAAACCGCA TTCATTGCCT TCGGCAGTAT GGTCGCCCCC 1201 GCATTGGCGG TTGCCGAAAA ACTGAACGCC ACCGTCGCCG ATATGCGCTT 1251 CGTCAAACCG ATAGACGAAG AGTTGATTGT CCGCCTTGCC CGAAGCCACG 1301 ACCGCATCGT TACCCTTGAA GAAAACGCCG AACAGGGCGG CGCAGGCGGC 1351 GCGGTGCTGG AAGTATTGGC GAAACACGGC ATCTGCAAAC CCGTTTTGCT 1401 TTTGGGCGTT GCCGATACCG TAACCGGACA CGGCGATCCG AAAAAACTTT 1451 TAGACGATTT GGGCTTGAGT GCCGAAGCGG TGGAACGGCG TGTGCGCGCG 1501 TGGCTGTCGG ATCGGGATGC GGCAAATTAA This corresponds to the amino acid sequence <SEQ ID 118; ORF 033>: m033.pep 1 MAAADKLLGS DRRSVAIIGD GAMTAGQAFE ALNCAXDMDV DLLWLNDNE 51 MSISPNVGAL PKYLASNWR DMHGLLSTVK AQTGKVLDKI PGAMEFAQKV 101 EHKIKTLAEE AEHAKQSLSL FENFGFRYTG PVDGHNVENL VDVLKDLRSR 151 KGPQLLHVIT KKGNGYKLAE NDPVKYHAVA NLPKESAAQM PSEKEPKPAA 201 KPTYTQVFGK WLCDRAAADS RLVAITPAMR EGSGLVEFEQ RFPDRYFDVG 251 IAEQHAVTFA GGLACEGMKP WAIYSTFLQ RAYDQLVHDI ALQNLPVLFA 301 VDRAGIVGAD GPTHAGLYDL SFLRCVPNMI VAAPSDENEC RLLLSTCYQA 351 DAPAAVRYPR GTGTGAPVSD GMETVEIGKG IIRREGEKTA FIAFGSMVAP 401 ALAVAEKLNA TVADMRFVKP IDEELIVRLA RSHDRIVTLE ENAEQGGAGG 451 AVLEVLAKHG ICKPVLLLGV ADTVTGHGDP KKLLDDLGLS AEAVERRVRA 501 WLSDRDAAN* The following partial DNA sequence was identified in N meningitidis <SEQ ID 119>: a033. seq 1 ATGGCGGCGG CGGACAAACA GTTGGGCAGC GACCGCCGCA GCGTCGCCAT 51 CATCGGCGAC GGCGCGATGA CGGCGGGTCA GGCGTTTGAA GCCTTGAACT 101 GCGCGGGCGA TATGGATGTG GATTTGCTGG TCGTCCTCAA CGACAACGAA 151 ATGTCGATTT CCCCCAACGT CGGTGCGTTG CCCAAATACC TTGCCAGCAA 201 CGTCGTGCGC GATATGCACG GACTGTTGAG TACCGTCAAA GCGCAAACGG 251 GCAAGGTATT AGACAAAATA CCCGGCGCGA TGGAGTTTGC CCAAAAAGTC 301 GAACATAAAA TCAAAACCCT TGCCGAAGAA GCCGAACACG CCAAACAGTC 351 ACTGTCTTTG TTTGAAAACT TCGGCTTCCG CTATACCGGC CCCGTGGACG 401 GACACAACGT CGAAAATCTG GTCGATGTAT TGGAAGACCT GCGCGGACGC 451 AAAGGCCCGC AGCTTCTGCA CGTCATCACC AAAAAGGGCA ACGGCTACAA 501 ACTCGCCGAA AACGATCCCG TCAAATACCA CGCCGTCGCC AACCTGCCTA 551 AAGAAAGCGC GGCGCAAATG CCGTCTGAAA AAGAACCCAA GCCCGCCGCC 601 AAACCGACCT ATACCCAAGT GTTCGGCAAA TGGCTGTGCG ACCGGGCGGC 651 GGCAGATTCC CGACTGGTTG CGATTACCCC CGCCATGCGC GAGGGCAGCG 701 GCTTGGTTGA GTTTGAACAA CGATTCCCCG ACCGCTATTT CGATGTCGGC 751 ATCGCCGAGC AGCACGCCGT TACCTTTGCC GGCGGTTTGG CTTGCGAAGG 801 GATGAAGCCC GTCGTGGCGA TTTATTCCAC CTTTTTACAA CGCGCCTACG 851 ACCAACTGGT GCACGACATC GCCCTGCAAA ACCTGCCCGT TTTGTTTGCC 901 GTCGACCGCG CGGGCATCGT CGGCGCGGAC GGCCCGACCC ATGCCGGTTT 951 GTACGATTTA AGCTTTTTGC GCTGCATTCC GAATATGATT GTCGCCGCGC 1001 CGAGCGATGA AAATGAATGC CGCCTGCTGC TTTCGACCTG CTATCAGGCA 1051 GACGCGCCCG CCGCCGTCCG CTATCCGCGC GGCACGGGTA CGGGCGTGCC 1101 GGTTTCAGAC GGCATGGAAA CCGTGGAAAT CGGCAAGGGC ATTATCCGCC 1151 GCGAAGGTGA GAAAACCGCA TTCATTGCCT TCGGCAGTAT GGTCGCCCCT 1201 GCATTGGCGG TCGCCGGAAA ACTGAACGCC ACCGTCGCCG ATATGCGCTT 1251 CGTCAAACCG ATAGACGAAG AGTTGATTGT CCGCCTTGCC CGAAGCCACG 1301 ACCGCATCGT TACCCTTGAA GAAAACGCCG AACAGGGCGG CGCAGGCAGC 1351 GCGGTGCTGG AAGTGTTGGC GAAACACGGC ATCTGCAAAC CCGTCTTGCT 1401 TTTGGGCGTT GCCGATACCG TAACCGGACA CGGCGATCCG AAAAAACTTT 1451 TAGACGATTT GGGCTTGAGT GCCGAAGCGG TGGAACGGCG TGTGCGCGCG 1501 TGGCTGTCGG ATCGGGATGC GGCAAATTAA This corresponds to the amino acid sequence <SEQ ID 120; ORF 033. a>: a033.pep 1 MAAADKQLGS DRRSVAIIGD GAMTAGQAFE ALNCAGDMDV DLLWLNDNE 51 MSISPNVGAL PKYLASNWR DMHGLLSTVK AQTGKVLDKI PGAMEFAQKV 101 EHKIKTLAEE AEHAKQSLSL FENFGFRYTG PVDGHNVENL VDVLEDLRGR 151 KGPQLLHVIT KKGNGYKLAE NDPVKYHAVA NLPKESAAQM PSEKEPKPAA 201 KPTYTQVFGK WLCDRAAADS RLVAITPAMR EGSGLVEFEQ RFPDRYFDVG 251 IAEQHAVTFA GGLACEGMKP WAIYSTFLQ RAYDQLVHDI ALQNLPVLFA 301 VDRAGIVGAD GPTHAGLYDL SFLRCIPNMI VAAPSDENEC RLLLSTCYQA 351 DAPAAVRYPR GTGTGVPVSD GMETVEIGKG IIRREGEKTA FIAFGSMVAP 401 ALAVAGKLNA TVADMRFVKP IDEELIVRLA RSHDRIVTLE ENAEQGGAGS 451 AVLEVLAKHG ICKPVLLLGV ADTVTGHGDP KKLLDDLGLS AEAVERRVRA 501 WLSDRDAAN* a033 98.4% identity over a 509 aa overlap 10 20 30 40 50 60 m033.pep MAAADKLLGSDRRSVAIIGDGAMTAGQAFEALNCAXDMDVDLLWLNDNEMSISPNVGAL llllll I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a033 MAAADKQLGSDRRSVAIIGDGAMTAGQAFEALNCAGDMDVDLLWLNDNEMSISPNVGAL 10 20 30 40 50 60 70 80 90 100 110 120 m033.pep PKYLASNWRDMHGLLSTVKAQTGKVLDKIPGAMEFAQKVEHKIKTLAEEAEHAKQSLSL I I I I I I I II I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I a033 PKYLASNWRDMHGLLSTVKAQTGKVLDKIPGAMEFAQKVEHKIKTLAEEAEHAKQSLSL 70 80 90 100 110 120 130 140 150 160 170 180 m033 . pep FENFGFRYTGPVDGHNVENLVDVLKDLRSRKGPQLLHVITKKGNGYKLAENDPVKYHAVA I I I I I I I I I I I I I I I I I I I I I I I I : I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a033 FENFGFRYTGPVDGHNVENLVDVLEDLRGRKGPQLLHVITKKGNGYKLAENDPVKYHAVA 130 140 150 160 170 180 190 200 210 220 230 240 m033.pep NLPKESAAQMPSEKEPKPAAKPTYTQVFGKWLCDRAAADSRLVAITPAMREGSGLVEFEQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I II I I I I I I I I I I I I I I I I I I I I I I I a033 NLPKESAAQMPSEKEPKPAAKPTYTQVFGKWLCDRAAADSRLVAITPAMREGSGLVEFEQ 190 200 210 220 230 240 250 260 270 280 290 300 m033 . pep RFPDRYFDVGIAEQHAVTFAGGLACEGMKPWAIYSTFLQRAYDQLVHDIALQNLPVLFA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a033 RFPDRYFDVGIAEQHAVTFAGGLACEGMKPWAIYSTFLQRAYDQLVHDIALQNLPVLFA 250 260 270 280 290 300 310 320 330 340 350 360 m033 . pep VDRAGIVGADGPTHAGLYDLSFLRCVPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPR I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a033 VDRAGIVGADGPTHAGLYDLSFLRCIPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPR 310 320 330 340 350 360 370 380 390 400 410 420 m033.pep GTGTGAPVSDGMETVEIGKGIIRREGEKTAFIAFGSMVAPALAVAEKLNATVADMRFVKP I I II I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a033 GTGTGVPVSDGMETVEIGKGIIRREGEKTAFIAFGSMVAPALAVAGKLNATVADMRFVKP 370 380 390 400 410 420 430 440 450 460 470 480 m033. pep IDEELIVRLARSHDRIVTLEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDP I I I I I I I I I I I I I I I I I I I I I I I I I I I I I: I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a033 IDEELIVRLARSHDRIVTLEENAEQGGAGSAVLEVLAKHGICKPVLLLGVADTVTGHGDP 430 440 450 460 470 480 490 500 510 m033.pep KKLLDDLGLSAEAVERRVRAWLSDRDAANX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a033 KKLLDDLGLSAEAVERRVRAWLSDRDAANX 490 500 510 Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 033 shows 98.4% identity over a 509 aa overlap with a predicted ORF (ORF 033.ng) from N gonorrhoeae: m033/g033 m033.pep MAAADKLLGSDRRSVAIIGDGAMTAGQAFEALΝCAXDMDVDLLWLΝDΝEMSISPΝVGAL 60 11 ! 11 ! 111 : ! I E 11 M I ! 11111 ! 11 i 1111111 11111 i 1111 ! 111 j i M 11 ! I II g 033 MAAADKLLGGDRRSVAI I GDGAMTAGQAFEALΝCAGDMDVDLLWLΝDΝEMS I S PΝVGAL 60 m033 . pep PKYLASΝWRDMHGLLSTVKAQTGKVLDKIPGAMEFAQKVEHKIKTLAEEAEHAKQSLSL 120 11111 M I II 11111 M 1111111 M 11 II 111111111111111111! 11 M I M 1111 g033 PKYLASΝWRDMHGLLSTVKAQTGKVLDKIPGAMEFAQKVEHKIKTLAEEAEHAKQSLSL 120 m033.pep FEΝFGFRYTGPVDGHΝVEΝLVDVLKDLRSRKGPQLLHVITKKGΝGYKLAEΝDPVKYHAVA 180 M I M 1111111 M M 1111 M M 1111! i 1111 M M M 1111 II 111111 M I II M I g033 FEΝFGFRYTGPVDGHΝVEΝLVDVLKDLRSRKGPQLLHVITKKGΝGYKLAEΝDPVKYHAVA 180 m033.pep ΝLPKESAAQMPSEKEPKPAAKPTYTQVFGKWLCDRAAADSRLVAITPAMREGSGLVEFEQ 240 I I II = 1111111111111 M 111111 M M II 111 I II 1111111111111 M 11111 g033 ΝLPKEGGAQMPSEKEPKPAAKPTYTQVFGKWLCDRAAADSRLVAITPAMREGSGLVEFEQ 240 m033 . pep RFPDRYFDVGIAEQHAVTFAGGLACEGMKPWAIYSTFLQRAYDQLVHDIALQΝLPVLFA 300 g033 RFPDRYFDVGIAEQHAVTFAGGLACEGMKPWAIYSTFLQRAYDQLVHDIALQΝLPVLFA 300 m033 . pep VDRAGIVGADGPTHAGLYDLSFLRCVPΝMIVAAPSDEΝECRLLLSTCYQADAPAAVRYPR 360 g033 VDRAGIVGADGPTHAGLYDLSFLRCVPΝMIVAAPSDEΝECRLLLSTCYQADAPAAVRYPR 360 m033.pep GTGTGAPVSDGMETVEIGKGIIRREGEKTAFIAFGSMVAPALAVAEKLΝATVADMRFVKP 420 g033 GTGTGAPVSDGMETVEIGKGIIRREGEKTAFIAFGSMVATALAVAEKLΝATVADMRFVKP 420 m033.pep IDEELIVRLARSHDRIVTLEEΝAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDP 480 MM g033 IDEELIVRLARSHDRIVTLEEΝAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTEHGDP 480 m033.pep KKLLDDLGLSAEAVERRVRAWLSDRDAANX 510 II gO33 KKLLDDLGLSAEAVERRVREWLPDRDAANX 510 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 121>: g034.seq 1 ATGAGCCGTT TATGGTTTTT TGCCGTAAAA AACATTATAA TCCGCCTTAT 51 TTACCTATTG CCCAAGGAGA CACAAATGGC ACTCGTATCC ATGCGCCAAC 101 TGCTTGACCA CGCCGCCGAA AACAGCTACG GCCTGCCCGC GTTCAACGTC 151 AACAACCTCG AACAAATGCG CGCCATTATG GAAGCCGCCG ACCAAGTCAA 201 CGCGCCCGTC ATCGTACAGG CGAGCGCAGG TGCGCGCAAA TACGcggGCG 251 CGCCGTTTTT GCGCCACCTG ATTCTGGCGG CAGTCGAAGA ATTTCCGCAC 301 ATCCCCGTCG TGATGCACCA AGACCACGGC GCATCGCCCG ACGTgtgCCA 351 ACGCTCCATC CAACTGGGCT TCTCCTCCGT GATGATGGAC GGCTCTTTGC 401 TCGAAGACGG CAAAACCCCT TCTTCTTACG AATACAACGT CAACGCCACC 451 CGTACCGTCG TCAACTTCTC CCACGCCTGC GGCGTGTCCG TCGAAGGCGA 501 AATCGGCGTA TTGGGCAACC TCGAAACCGG CGAAGCAGGC GAAGAAGACG 551 GAGTGGGCGC GGCAGGCAAA CTCTCACACG ACCAAATGCT CACCAGCGTT 601 GAAGATGCCG TGCGTTTCGT TAAAGATACC GGCGTTGACG CATTGGCGAT 651 TGCCGTCGGC ACCAGCCACG GCGCATACAA ATTCACCCGT CCGCCCACAG 701 GCGACGTATT GCGTATCGAC CGCATCAAGG AAATCCACCA AGCCCTGCCC 751 AATACACACA TCGTGATGCA CGgctCCAGC TCCGTTCCGC AAGAatgGCT 801 GAAAGTCATC AACGAATACG GCGGCAATAT CGGCGAAACC TACGGCGTGC 851 CGGTTGAAGA AATCGTCGAA GGCATCAAAC ACGGCGTGCG CAAAGTCAAC 901 ATCGATACCG ACCTGCGCCT CGCTTCCACC GGCGCGGTAC GCCGCTACCT 951 TGCCGAAAAC CCGTCCGACT TTGATCCGCG CAAATACTTG GGCAAAACCA 1001 TTGAAGCGAT GAAGCAAATC TGCCTCGACC GTTATCTTGC GTTCGGTTGC 1051 GAAGGTCAGG CAGGCAAAAT CAAACCTGTT TCGTTGGAAA AAATGGCAAG 1101 CCGTTATGCC AAGGGCGAAT TGAACCAAAT CGTCAAATAA This corresponds to the amino acid sequence <SEQ ID 122; ORF 034.ng>: g034.pep 1 MSRLWFFAVK NIIIRLIYLL PKETQMALVS MRQLLDHAAE NSYGLPAFNV 51 NNLEQMRAIM EAADQVNAPV IVQASAGARK YAGAPFLRHL ILAAVEEFPH 101 IPWMHQDHG ASPDVCQRSI QLGFSSVMMD GSLLEDGKTP SSYEYNVNAT 151 RTWNFSHAC GVSVEGEIGV LGNLETGEAG EEDGVGAAGK LSHDQMLTSV 201 EDAVRFVKDT GVDALAIAVG TSHGAYKFTR PPTGDVLRID RIKEIHQALP 251 NTHIVMHGSS SVPQEWLKVI NEYGGNIGET YGVPVEEIVE GIKHGVRKVN 301 IDTDLRLAST GAVRRYLAEN PSDFDPRKYL GKTIEAMKQI CLDRYLAFGC 351 EGQAGKIKPV SLEKMASRYA KGELNQIVK* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 123>: m034.seq (partial) 1 ATGAGCTGTT TATGGTTTTT TGCTGTAAAA AACATTATAA TCCGCCTTAT 51 TTACCTATTG CCCAAGGAGA CACAAATGGC ACTCGTATCC ATGCGCCAAC 101 TGCTTGATCA TGCTGCCGAA ACAGCTACG GCyTGCCGGC GTTCAACGTC 151 AACAACCTCG wACAGATGCG CGCCATCATG GAGGCTGCAG ACCAAGTCGA 201 CGCCCCCGTC ATCGTACAGG CGAGTGCCGG TGCGCGCAAA TATGCGGGTG 251 CGCCGTTTTT ACGCCACCTG ATTTTGGCGG CTGTCGAAGT ATTTCCACAC 301 ATCCCCGTCG TCATGCACCA AGACCACGGC GCATCACCCG ACGTGTGCCA 351 ACGCTCCATC CAACTGGGCT TCTCCTCTGT AATGATGGAC GGCTCGCTGA 401 TGGAAGACGG CAAAACCCCT TCTTCTTACG AATACAACGT CAACGCCACA 451 CGTACCGTGG TTAACTTCTC CCACGCTTGC GGCGTATCCG TTGAAGGCGA 501 AATCGGCGTA TTGGGCAACC TCGAAACCGG CGATGCAGGC GAAGAAGACG 551 GTGTAGGCGC AGTGGGCAAA CTTTCCCACG ACCAAATGCT GACCAGCGTC 601 GAAGATGCCG TATGTTTCGT TAAAGATACC GGCGTTGACG CATTGGCTAT 651 TGCCGTCGGC ACCAGCCACG GCGCATACAA ATTCACCCGT CCGCCCACAG 701 GCGATGTATT ACGTATCGAC CGCATCAAAG AAATCCACCA AGCCCTGCCC 751 AATACACACA TCGTGATGCA C... This corresponds to the amino acid sequence <SEQ ID 124; ORF 034>: mO3 .pep (partial) 1 MSCLWFFAVK NIIIRLIYLL PKETQMALVS MRQLLDHAAE XSYGLPAFNV 51 NNLXQMRAIM EAADQVDAPV IVQASAGARK YAGAPFLRHL ILAAVEVFPH 101 IPWMHQDHG ASPDVCQRSI QLGFSSVMMD GSLMEDGKTP SSYEYNVNAT 151 RTWNFSHAC GVSVEGEIGV LGNLETGDAG EEDGVGAVGK LSHDQMLTSV 201 EDAVCFVKDT GVDALAIAVG TSHGAYKFTR PPTGDVLRID RIKEIHQALP 251 NTHIVMH... The following partial DNA sequence was identified in N. meningitidis <SEQ ID 125>: a034. seq 1 ATGAGCCGTT TATGGTTTTT TGCCGCAAAA AACATTATAA TCCGCCTTAT 51 TTACCTATTG CCCAAGGAGA CACAAATGGC ACTCGTATCC ATGCGCCAAC 101 TGCTTGATCA TGCTGCCGAA AACAGCTACG GCCTGCCCGC GTTCAACGTC 151 AACAACCTCG AACAAATGCG CGCCATTATG GAAGCCGCCG ACCAAGTCAA 201 CGCGCCCGTC ATCGTACAGG CGAGCGCAGG TGCGCGCAAA TACGCGGGCG 251 CGCCGTTTTT GCGCCACCTG ATTTTGGCGG CTGTCGAAGA ATTTCCGCAC 301 ATCCCCGTCG TGATGCACCA AGACCACGGC GCATCGCCCG ACGTGTGCCA 351 ACGCTCCATC CAACTGGGCT TTTCCTCCGT GATGATGGAC GGCTCGCTGA 401 TGGAAGACGG CAAAACCCCT TCTTCTTATG AATACAACGT CAACGCCACC 451 CGTACCGTGG TTAATTTCTC CCACGCCTGC GGCGTATCCG TTGAAGGCGA 501 AATCGGCGTA TTGGGCAACC TCGAAACTGG CGAAGCCGGC GAAGAAGACG 551 GTGTAGGCGC AGTGGGCAAA CTTTCCCACG ACCAAATGCT CACCAGCGTC 601 GAAGATGCCG TGCGTTTCGT TAAAGATACC GGCGTTGACG CATTGGCGAT 651 TGCCGTCGGC ACCAGCCACG GCGCGTACAA ATTCACCCGT CCGCCCACAG 701 GCGACGTGTT GCGTATCGAC CGCATCAAAG AAATCCACCA AGCCCTGCCC 751 AATACACACA TCGTGATGCA CGGCTCCAGC TCCGTTCCGC AAGAATGGCT 801 GAAAGTCATC AACGAATACG GCGGCAATAT CGGCGAAACC TACGGCGTGC 851 CGGTTGAAGA AATCGTCGAA GGCATCAAAC ACGGCGTGCG TAAAGTCAAC 901 ATCGATACCG ACTTGCGCCT TGCTTCCACC GGCGCGGTAC GCCGCTACCT 951 TGCCGAAAAC CCGTCCGACT TCGATCCGCG CAAATATTTG AGCAAAACCA 1001 TTGAAGCGAT GAAGCAAATC TGCCTCGACC GCTACCTCGC GTTCGGTTGC 1051 GAAGGTCAGG CAGGCAAAAT CAAACCGGTT TCCTTGGAAA AAATGGCAAA 1101 CCGTTATGCC AAGGGCGAAT TGAACCAAAT CGTCAAATAA This corresponds to the amino acid sequence <SEQ ID 126; ORF 034.a>: a034.pep 1 MSRLWFFAAK NIIIRLIYLL PKETQMALVS MRQLLDHAAE NSYGLPAFNV 51 NNLEQMRAIM EAADQVNAPV IVQASAGARK YAGAPFLRHL ILAAVEEFPH 101 IPWMHQDHG ASPDVCQRSI QLGFSSVMMD GSLMEDGKTP SSYEYNVNAT 151 RTWNFSHAC GVSVEGEIGV LGNLETGEAG EEDGVGAVGK LSHDQMLTSV 201 EDAVRFVKDT GVDALAIAVG TSHGAYKFTR PPTGDVLRID RIKEIHQALP 251 NTHIVMHGSS SVPQEWLKVI NEYGGNIGET YGVPVEEIVE GIKHGVRKVN 301 IDTDLRLAST GAVRRYLAEN PSDFDPRKYL SKTIEAMKQI CLDRYLAFGC 351 EGQAGKIKPV SLEKMANRYA KGELNQIVK* m034/a034 ( 96.9%) identity over a 257 aa overlap 10 20 30 40 50 60 m034 .pep MSCLWFFAVKNIIIRLIYLLPKETQMALVSMRQLLDHAAEXSYGLPAFNVNNLXQMRAIM II I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I llllll a034 MSRLWFFAAKNIIIRLIYLLPKETQMALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIM 10 20 30 40 50 60 70 80 90 100 110 120 m034.pep EAADQVDAPVIVQASAGARKYAGAPFLRHLILAAVEVFPHIPWMHQDHGASPDVCQRSI I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a034 EAADQVNAPVIVQASAGARKYAGAPFLRHLILAAVEEFPHIPWMHQDHGASPDVCQRSI 70 80 90 100 110 120 130 140 150 160 170 180 m034. pep QLGFSSVMMDGSLMEDGKTPSSYEYNVNATRTWNFSHACGVSVEGEIGVLGNLETGDAG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I II : I I a034 QLGFSSVMMDGSLMEDGKTPSSYEYNVNATRTWNFSHACGVSVEGEIGVLGNLETGEAG 130 140 150 160 170 180 190 200 210 220 230 240 m034 . pep EEDGVGAVGKLSHDQMLTSVEDAVCFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRID I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a034 EEDGVGAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRID 190 200 210 220 230 240 250 m034.pep RIKEIHQALPNTHIVMH I I I I I I I I I I I I I I I I I a034 RIKEIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVN 250 260 270 280 290 300 Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 034 shows 96.5% identity over a 257 aa overlap with a predicted ORF (ORF 034.ng) from N. gonorrhoeae: m034/g034 m034 . pep MSCLWFFAVKNII IRLIYLLPKETQMALVSMRQLLDHAAEXSYGLPAFNVNNLXQMRAIM 60 II g034 MSRLWFFAVKNIIIRLIYLLPKETQMALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIM 60 m034.pep EAADQVDAPVIVQASAGARKYAGAPFLRHLILAAVEVFPHIPWMHQDHGASPDVCQRSI 120 M M I h l M M M M M M M M I M I M M M M 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 g034 EAADQVNAPVIVQASAGARKYAGAPFLRHLILAAVEEFPHIPWMHQDHGASPDVCQRSI 120 m034.pep QLGFSSVMMDGSLMEDGKTPSSYEYNVNATRTWNFSHACGVSVEGEIGVLGNLETGDAG 180 M M M M M M h l M M M M M M M M M M M M M M M M I M I M M I h l l g034 QLGFSSVMMDGSLLEDGKTPSSYEYNVNATRTWNFSHACGVSVEGEIGVLGNLETGEAG 180 m034 . pep EEDGVGAVGKLSHDQMLTSVEDAVCFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRID 240 gO34 EEDGVGAAGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRID 240 m034.pep RIKEIHQALPNTHIVMH 257 g034 RIKEIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVN 300 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 127>: g036.seq 1 ATGCTGAAGC CGTGTTTGGT ATACAGTGCC TGTGCGGCGG cgttgcCTGC 51 GCGGACTTCG AGCAGCAGGC GTTGCGTGCC TTCGGGCAGA TGTGCGTACC 101 AATATTCGAG CAGGGCGGAC GCAACGCCCC GTCGGCGGCA TTCGGGCGCG 151 GTGGCAATCA GGTGCAGTTC GGATTCGTCG GGCAGGTTCT GCCAAACGAT 201 AAAGGCGGCA ATCCTGCCGT CTTTTTCCGC AAGGAAAACC TGTTCGGACG 251 GCGAAACAAG CGCGGACTCA AATTGGCGTT GCGTCCACGC GGACGGGTTG 301 CAGACGGTAT CGAGCGCGGC CAGTGCGGCG CAGTCGGACG GTGAGGCTGG 351 GCGGATGTTC ATGTTCGTGC CTTCCGTTCC GCCTGTTCTT TGGCAGTCAG 401 GGCGATTTTG TTGCGGACGT AGAGCAGTTC GGCGTGTGCC GCGCCAGTTG 451 CGGGATAGCC GCCGCCGAGG GCGAGCGCGA GAAAATCGGC GGCGGTCGGC 501 ATATCGGGTT TGCCTGAGAA GGGCGGACGG TTTTCCAGTG CGAACGCACT 551 GCCGATGCCG TCTGAAAAGA CGTACCCCTC GGGGAGGGCA ATGTCTGCCG 601 CCCTACCGAC TTGATAATCG CTCAAACGGC GGCGGTTCAG CGTGTCGAAC 651 CACGCATAAA ACACTTCGCC CATACGCGCG TCCGCAGCGG CGAGTATGCA 701 GCTTTGCGGC GGCGGCAGCG AGGCGGCGGC ATCGAGCGTG GGGATGCCGA 751 TTAAAGGCGT GTCGAACGGC GTTGCCAAAC CTTGCGCCAC GCCGATGCCG 801 ATACGCAGTC CGGTAA This corresponds to the amino acid sequence <SEQ ID 128; ORF 036.ng>: g036.pep 1 MLKPCLVYSA CAAALPARTS SSRRCVPSGR CAYQYSSRAD ATPRRRHSGA 51 VAIRCSSDSS GRFCQTIKAA ILPSFSARKT CSDGETSADS ΝWRCVHADGL 101 QTVSSAASAA QSDGEAGRMF MFVPSVPPVL WQSGRFCCGR RAVRRVPRQL 151 RDSRRRGRAR EΝRRRSAYRV CLRRADGFPV RTHCRCRLKR RTPRGGQCLP 201 PYRLDΝRSΝG GGSACRTTHK TLRPYARPQR RVCSFAAAAA RRRHRAWGCR 251 LKACRTALPΝ LAPRRCRYAV R* The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 129>: m036. seq 1 ATGCTGAAGC CGTGCGCCGT GTACAGTGCC TGTGCGGCGG TGTTGCCTGC 51 ACGGACTTCG AGCAGCAGGC GTTGCGTGTC TTCGGGCAGA TGTGTGAACC 101 AATATTCGAG CAGGGCGGAC GCAATTCCTT GGCGGCGGCA TTCGGGCGCG 151 GTGGCAATCA GGTGCAGTTC GGATTCGTCG GGCAGGTTCT GCCAAACGAT 201 AAAGGCGGCA ATCCCg.CGT CTTTTTCCGC AAGGAAAACC TGTTCGGACG 251 GCGAAACCAG TGCGGACTCA AATTGGCGTT GCGTCCATGC GGACGGGTTG 301 CAGACGGCAT CGAGTGCGGC CAGCTCCTCA CAATCGGCAC AAACGGCACG 351 GCGGATGTTC ACGGGCGCGC TCTCCGTTCG GCCTGTTCTT TGGCAGTCAG 401 GGCGATTTTG TTGCGGACGT AGAGCAAACC GGCGTGTGCG GCATGGACGG 451 CAGGATAACC GCCCTTGGCT GCCAATGCGA GAAAGTCGGC GGCAGTCGGC 501 ATATCCGGTC TGCCTGAGAA CGGCGGAGCT TCTTCCAGCG CGAACGCGCT 551 GCCTATGCCG TCTGAAAAGG CGCATCCCTC CGGCAGCCGG ATGTCTGCCG 601 CCCGCCCGAC CTGATAATCG CTCAAACGGT GGCAGTTCAG CGTATCGAAC 651 CATGCATAAA ACACTTCGCC CATACGAGCG TCCGTAGCGG CAAGGATGCA 701 GCTTTGCGGC GGCGGCAGCG AGGCGGCGGC ATCGAGCGAG GGTACGCCGA 751 TTAAGGGGGT ATCAAACGGC GTTGCCAAAC CCTGAGCTAC ACCGATGCCG 801 ATACGCAGTC CGGTAA This corresponds to the amino acid sequence <SEQ ID 130; ORF 036>: m036.pep 1 MLKPCAVYSA CAAVLPARTS SSRRCVSSGR CVNQYSSRAD AIPWRRHSGA 51 VAIRCSSDSS GRFCQTIKAA IPXSFSARKT CSDGETSADS NWRCVHADGL 101 QTASSAASSS QSAQTARRMF TGALSVRPVL WQSGRFCCGR RANRRVRHGR 151 QDNRPWLPMR ESRRQSAYPV CLRTAELLPA RTRCLCRLKR RIPPAAGCLP 201 PARPDNRSNG GSSAYRTMHK TLRPYERP*R QGCSFAAAAA RRRHRARVRR 251 LRGYQTALPN PELHRCRYAV R* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 131>: a036. seq 1 ATGCTGAAGC CGTGCGCCGT GTACAGTGCC TGTGCGGCGG TGTTGCCTGC 51 ACGGACTTCG AGCAGCAGGC GTTGCGTGTC TTCGGGCAGA TGTGTGAACC 101 AATATTCGAG CAGGGCGGAC GCAATTCCTT GGCGGCGGCA TTCGGGCGCG 151 GTGGCAATCA GGTGCAGTTC GGATTCGTCG GGCAGGTTCT GCCAAACGAT 201 AAAGGCGGCA ATCCCGCCGT CTTTTTCCGC AAGGAAAACC TGTTCGGACG 251 GCGAAACCAG TGCGGACTCA AATTGGCGTT GCGTCCACGC GGACGGGTTG 301 CAGACGGCAT CGAGCGCGGC GAGTGCGGCG CAATCGGCAT AAACGGCGCG 351 GCGGATGTTC ACAGGCGCGC CCTCCGTTCC GCCTGTTCTT TGGCAGTCAA 401 GGCGATTTTG TTGCGGACGT AGAGCAGCTC GGCGTGTGCC GCAGCGACGG 451 CGGGAAAACC GCCTTCAGCC GCCAGATTGA GGAAGTCGGC GGCGGTCGGC 501 ATATCGGGTT TGCCTGAGAA GGGCGGACGG TTTTCCAGCG CGAACGCATT 551 GCCGATGCCG TCTGAAAAGG CGCATCCTTC CGGCAGCCGG ATGTCTGCCG 601 CCCGACCGAC CTGATAATCG CTCAAACGGC GGCGGTTCAG CGTGTCGAAC 651 CATGCATAAA ACACTTCGCC CATACGTGCG TCCGCAGCGG CAAGGATGCA 701 GCTTTGCGGC GGCGGCAGCG AGGCGGCGGC ATCGAGCGAG GGTACGCCGA 751 TTAAAGGAGT ATCAAACGGC GTTGCCAAAC CTTGCGCCAC GCCGATGCCG 801 ATACGCAGTC CCGTAA This corresponds to the amino acid sequence <SEQ ID 132; ORF 036.a>: a036.pep 1 MLKPCAVYSA CAAVLPARTS SSRRCVSSGR CVNQYSSRAD AIPWRRHSGA 51 VAIRCSSDSS GRFCQTIKAA IPPSFSARKT CSDGETSADS NWRCVHADGL 101 QTASSAASAA QSA*TARRMF TGAPSVPPVL WQSRRFCCGR RAARRVPQRR 151 RENRLQPPD* GSRRRSAYRV CLRRADGFPA RTHCRCRLKR RILPAAGCLP 201 PDRPDNRSNG GGSACRTMHK TLRPYVRPQR QGCSFAAAAA RRRHRARVRR 251 LKEYQTALPN LAPRRCRYAV P* m036/a036 85.6%) identity over a 270 aa overlap 10 20 30 40 50 60 m036.pep MLKPCAVYSACAAVLPARTSSSRRCVSSGRCVNQYSSRADAIPWRRHSGAVAIRCSSDSS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a036 MLKPCAVYSACAAVLPARTSSSRRCVSSGRCVNQYSSRADAIPWRRHSGAVAIRCSSDSS 10 20 30 40 50 60 70 80 90 100 110 120 m036.pep GRFCQTIKAAIPXSFSARKTCSDGETSADSNWRCVHADGLQTASSAASSSQSAQTARRMF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : : I I I llllll a036 GRFCQTIKAAIPPSFSARKTCSDGETSADSNWRCVHADGLQTASSAASAAQSAXTARRMF 70 80 90 100 110 120 130 140 150 160 170 180 m036.pep TGALSVRPVLWQSGRFCCGRRANRRVRHGRQDNRPWLPMRESRRQSAYPVCLRTAELLPA III II llllll I I I I I I I I III : I :: I I I I I I : I I I I I I I I : : I I a036 TGAPSVPPVLWQSRRFCCGRRAARRVPQRRRENRLQPPDXGSRRRSAYRVCLRRADGFPA 130 140 150 160 170 180 190 200 210 220 230 240 m036.pep RTRCLCRLKRRIPPAAGCLPPARPDNRSNGGSSAYRTMHKTLRPYERPXRQGCSFAAAAA I I : I lllllll I I I I I I I I 111111111:11 I I I I I I I I I I II I I I I I I I I I I I a036 RTHCRCRLKRRILPAAGCLPPDRPDNRSNGGGSACRTMHKTLRPYVRPQRQGCSFAAAAA 190 200 210 220 230 240 250 260 270 m036. pep RRRHRARVRRLRGYQTALPNPELHRCRYAVRX I I I I I I I I I I I : lllllll : I I I I I I a036 RRRHRARVRRLKEYQTALPNLAPRRCRYAVPX 250 260 270 Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 036 shows 74.9% identity over a 271 aa overlap with a predicted ORF (ORF 036.ng) from N gonorrhoeae: m036/g036 10 20 30 40 50 60 m036.pep MLKPCAVYSACAAVLPARTSSSRRCVSSGRCVΝQYSSRADAIPWRRHSGAVAIRCSSDSS I I I I I I I I I I I I : I I I I I I I I I I I I MM: | | | II I I I I I II I I II I I I I I I II I g036 MLKPCLVYSACAAALPARTSSSRRCVPSGRCAYQYSSRADATPRRRHSGAVAIRCSSDSS 10 20 30 40 50 60 70 80 90 100 110 120 m036.pep GRFCQTIKAAIPXSFSARKTCSDGETSADSΝWRCVHADGLQTASSAASSSQSAQTARRMF II II I II II I I I II II I I II I II II I I I II II I I I I I I II: I I I II:: I I I Ml g036 GRFCQTIKAAILPSFSARKTCSDGETSADSΝWRCVHADGLQTVSSAAΞAAQSDGEAGRMF 70 80 90 100 110 120 130 140 150 160 170 180 m036.pep TGALSVRPVLWQSGRFCCGRRAΝRRVRHGRQDΝRPWLPMRESRRQSAYPVCLRTAELLPA : II II I II II II II I I II III : : I : I I I: I I : I II I II I I : : I : g036 MFVPSVPPVLWQSGRFCCGRRAVRRVPRQLRDSRRRGRAREΝRRRSAYRVCLRRADGFPV 130 140 150 160 170 180 190 200 210 220 230 240 m036.pep RTRCLCRLKRRIPPAAGCLPPARPDΝRSΝGGSSAYRTMHKTLRPYERPXRQGCSFAAAAA I I: I llllll I :: I II I I II II I I I : II II lllllll II I : II I II II I g036 RTHCRCRLKRRTPRGGQCLPPYRLDΝRSΝGGGSACRTTHKTLRPYARPQRRVCSFAAAAA 190 200 210 220 230 240 250 260 270 mO36. pep RRRHRARVRRLRGYQTALPΝPELHRCRYAVRX llllll II:: : I II II : II II I II I g036 RRRHRAWGCRLKACRTALPΝLAPRRCRYAVRX 250 260 270 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 133>: m036-l . seq 1 ATGCTGAAGC CGTGCGCCGT GTACAGTGCC TGTGCGGCGG TGTTGCCTGC 51 ACGGACTTCG AGCAGCAGGC GTTGCGTGTC TTCGGGCAGA TGTGTGAACC 101 AATATTCGAG CAGGGCGGAC GCAATTCCTT GGCGGCGGCA TTCGGGCGCG 151 GTGGCAATCA GGTGCAGTTC GGATTCGTCG GGCAGGTTCT GCCAAACGAT 201 AAAGGCGGCA ATCCCGCCGT CTTTTTCCGC AAGGAAAACC TGTTCGGACG 251 GCGAAACCAG TGCGGACTCA AATTGGCGTT GCGTCCATGC GGACGGGTTG 301 CAGACGGCAT CGAGTGCGGC CAGCTCCTCA CAATCGGCAC AAACGGCACG 351 GCGGATGTTC ACGGGCGCGC TCTCCGTTCG GCCTGTTCTT TGGCAGTCAG 401 GGCGATTTTG TTGCGGACGT AGAGCAAACC GGCGTGTGCG GCATGGACGG 451 CAGGATAACC GCCCTTGGCT GCCAATGCGA GAAAGTCGGC GGCAGTCGGC 501 ATATCCGGTC TGCCTGAGAA CGGCGGAGCT TCTTCCAGCG CGAACGCGCT 551 GCCTATGCCG TCTGAAAAGG CGCATCCCTC CGGCAGCCGG ATGTCTGCCG 601 CCCGCCCGAC CTGATAATCG CTCAAACGGT GGCAGTTCAG CGTATCGAAC 651 CATGCATAAA ACACTTCGCC CATACGAGCG TCCGTAG This corresponds to the amino acid sequence <SEQ ID 134; ORF 0036-l>: m036-l .pep 1 MLKPCAVYSA CAAVLPARTS SSRRCVSSGR CVNQYSSRAD AIPWRRHSGA 51 VAIRCSSDSS GRFCQTIKAA IPPSFSARKT CSDGETSADS NWRCVHADGL 101 QTASSAASSS QSAQTARRMF TGALSVRPVL WQSGRFCCGR RANRRVRHGR 151 QDNRPWLPMR ESRRQSAYPV CLRTAELLPA RTRCLCRLKR RIPPAAGCLP 201 PARPDNRSNG GSSAYRTMHK TLRPYERP* m036-ι/g036 76.8%> identity in 228 aa overlap 10 20 30 40 50 60 m036-l.pep MLKPCAVYSACAAVLPARTSSSRRCVSSGRCVNQYSSRADAIPWRRHSGAVAIRCSSDSS II II I I I I I I I I : II II I II I I I II MM: I II II II I I I II II I I I I II II II I g036 MLKPCLVYSACAAALPARTSSSRRCVPSGRCAYQYSSRADATPRRRHSGAVAIRCSSDSS 10 20 30 40 50 60 70 80 90 100 110 120 m036-l.pep GRFCQTIKAAIPPSFSARKTCSDGETSADSNWRCVHADGLQTASSAASSSQSAQTARRMF I I II I II II I I I II II I II II II I I II I I I I II I I II II I I : I II II :: II I III g036 GRFCQTIKAAILPSFSARKTCSDGETSADSNWRCVHADGLQTVSSAASAAQSDGEAGRMF 70 80 90 100 110 120 130 140 150 160 170 180 m036-l.pep TGALSVRPVLWQSGRFCCGRRANRRVRHGRQDNRPWLPMRESRRQSAYPVCLRTAELLPA : II II II II II II I II I I III : : I : I II : II : I I I II II I : : I : g036 MFVPSVPPVLWQSGRFCCGRRAVRRVPRQLRDSRRRGRARENRRRSAYRVCLRRADGFPV 130 140 150 160 170 180 190 200 210 220 229 m036-l .pep RTRCLCRLKRRIPPAAGCLPPARPDNRSNGGSSAYRTMHKTLRPYERPX II : I llllll I : : I II I I II II I I I : I I II lllllll II g036 RTHCRCRLKRRTPRGGQCLPPYRLDNRSNGGGSACRTTHKTLRPYARPQRRVCSFAAAAA 190 200 210 220 230 240 g036 RRRHRAWGCRLKACRTALPNLAPRRCRYAVRX 250 260 270 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 135>: g038. seq 1 ATGACTGATT TCCGCCAAGA TTTCCTCAAA TTCTCCCTCG CCCAAAATGT 51 TTTGAAATTC GGCGAATTTA CCACCAAAGC CGGACGGCGG TCGCCCTATT 101 TCTTCAATGC CGGCCTCTTC AACGACGGCG CGTCCACGCT GCAACTGGCA 151 AAATTCTATG CACAATCCAT CATTGAAAGC GGCATCCGAT TCGATATGCT 201 GTTCGGCCCC GCCTACAAAG GCATTATTTT GGCGGCGGCA ACCGCGATGA 251 TGCTGGCGGA AAAAGGCGTG AACGTCCCGT TTGCCTACAA CCGCAAAGAA 301 GCCAAAGACC GCGGCGAAGG CGGCGTGTTG GTCGGCGCGC CGCTTAAAGG 351 GCGCGTGCTG ATTATCGACG ACGTGATTTC CGCCGGCACA TCCGTACGCG 401 AATCAATCAA ACTGATTGAA GCGGAGGGTG CAACCCCCGC CGGTGTCGCC 451 ATCGCGCTCG ACCGCATGGA AAAAGGCACG GGTAAATTGT CCGCCGTTCA 501 GGAAGTGGAA AAACAATACG GCCTGCCCGT CGCCCCCATC GCCAGCCTGA 551 ACGATTTGTT TATCCTGTTG CAAAACAACC CCGAATTCGG ACAGTTCCTC 601 GAACCCGTCC GCACCTACCG CCGGCAGTAC GGCGTAGAAT AA This corresponds to the amino acid sequence <SEQ ID 136; ORF 038.ng>: g038.pep 1 MTDFRQDFLK FSLAQNVLKF GEFTTKAGRR SPYFF AGLF NDGASTLQLA 51 KFYAQSIIES GIRFDMLFGP AYKGIILAAA TAMMLAEKGV NVPFAYNRKE 101 AKDRGEGGVL VGAPLKGRVL IIDDVISAGT SVRESIKLIE AEGATPAGVA 151 IALDRMEKGT GKLSAVQEVE KQYGLPVAPI ASLNDLFILL QNNPEFGQFL 201 EPVRTYRRQY GVE* The following partial DNA sequence was identified in N meningitidis <SEQ ID 137>: m038. seq 1 ATGACCGATT TCCGCCAAGA TTTCCTCAAA TTCTCCCTCG CCCAAAATGT 51 TTTGAAATTC GGCGAATTTA CCACCAAGGC AGGACGGCGG TCGCCCTATT 101 TCTTCAATGC CGGCCTCTTT AACGACGGCT TGTCCACGCT GCAACTGGCA 151 AAATTTTACG CACAATCCAT CATTGAAAGC GGCATCCGAT TCGATATGCT 201 GTTCGGTCCC GCCTACAAAG GCATTATTTT GGCGGCGGCA ACCGCGATGA 251 TGCTGGCGGA AAAAGGCGTG AACGTCCCGT TTGCCTACAA CCGCAAAGAA 301 GCCAAAGACC ACGGCGAAGG CGGCGTGTTG GTCGGCGCGC CGCTTAAAGG 351 GCGCGTGCTG ATTATCGACG ACGTGATTTC CGCCGGCACA TCCGTACGCG 401 AATCGATCAA ACTGATTGAA GCGGAGGGTG CAACCCCcGC CGGTGTCGCC 451 ATCGCGCTCG ATCGCATGGA AAAAGGCACG GGTGAATTGA GCGCGGTTCA 501 GGAAGTGGAr AAACAATACG GkCTGCCCGT CGCCCCCATC GCCAGCCTGA 551 ACGATTTGTT TATTCTGTTG CAAAACAACC CCGAATTCGG ACAGTTCCTC 601 GAACCCGTCC GAGCCTACCG TCGGCAGTAC GGCGTAGAAT AA This corresponds to the amino acid sequence <SEQ ID 138; ORF 038>: m038.pep 1 MTDFRQDFLK FSLAQNVLKF GEFTTKAGRR SPYFFNAGLF NDGLSTLQLA 51 KFYAQSIIES GIRFDMLFGP AYKGIILAAA TAMMLAEKGV NVPFAYNRKE 101 AKDHGEGGVL VGAPLKGRVL IIDDVISAGT SVRESIKLIE AEGATPAGVA 151 IALDRMEKGT GELSAVQEVE KQYGLPVAPI ASLNDLFILL QNNPEFGQFL 201 EPVRAYRRQY GVE* The following partial DNA sequence was identified in N meningitidis <SEQ ID 139>: a038. seq 1 ATGACCGATT TCCGCCAAGA TTTCCTCAAA TTCTCCCTCG CCCAAAATGT 51 TTTGAAATTC GGCGAATTCA CCACCAAAGC CGGACGGCGG TCGCCCTATT 101 TCTTCAATGC CGGCCTCTTT AACGACGGCT TGTCCACGCT GCAACTGGCA 151 AAATTTTACG CACAATCCAT CATTGAAAGC GGCATCCGAT TCGATATGCT 201 GTTCGGCCCC GCCTACAAAG GCATTATTTT GGCGGCGGCA ACCGCGATGA 251 TGCTGGCGGA AAAAGGCGTG AACGTCCCGT TTGCCTACAA CCGCAAAGAA 301 GCCAAAGACC ACGGCGAAGG CGGCGTGTTG GTCGGCGCGC CGCTTAAAGG 351 GCGCGTGCTG ATTATCGACG ACGTGATTTC CGCCGGCACA TCCGTACGCG 401 AATCGATCAA ACTGATTGAA GCGGAGGGTG CAACCCCCGC CGGTGTCGCC 451 ATCGCGCTCG ACCGCATGGA AAAAGGCACG GGTGAATTGA GCGCGGTTCA 501 GGAAGTGGAA AAACAATACG GCCTGCCCGT CGCCCCCATC GCCAGCCTGA 551 ACGATTTGTT TATTCTGTTG CAAAACAACC CCGAATTCGG ACAGTTCCTC 601 GAACCCGTCC GAGCCTACCG TCGGCAGTAC GGCGTAGAAT AA This corresponds to the amino acid sequence <SEQ ID 140; ORF 038. a>: a038.pep 1 MTDFRQDFLK FSLAQNVLKF GEFTTKAGRR SPYFFNAGLF NDGLSTLQLA 51 KFYAQSIIES GIRFDMLFGP AYKGIILAAA TAMMLAEKGV NVPFAYNRKE 101 AKDHGEGGVL VGAPLKGRVL IIDDVISAGT SVRESIKLIE AEGATPAGVA 151 IALDRMEKGT GELSAVQEVE KQYGLPVAPI ASLNDLFILL QNNPEFGQFL 201 EPVRAYRRQY GVE* m038/a038 100.0% identity over a 213 aa overlap 10 20 30 40 50 60 m038.pep MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGLSTLQLAKFYAQSIIES I II II I II II I II I I I I I I I I I II I II II II I I I I I II I II II I I II I II II I II II II I a038 MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGLSTLQLAKFYAQSIIES 10 20 30 40 50 60 70 80 90 100 110 120 m038. pep GIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAKDHGEGGVLVGAPLKGRVL I II II II I I II II I II I I II I II II I I II II I II I II I I I I I I I I I I II II II II I I II I a038 GIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAKDHGEGGVLVGAPLKGRVL 70 80 90 100 110 120 130 140 150 160 170 180 m038. pep IIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGTGELSAVQEVEKQYGLPVAPI I I I I II I II I I I II II II II I II I II I I I I II I I I I II I I I I II I I I II I I I II I II I II a038 IIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGTGELSAVQEVEKQYGLPVAPI 130 140 150 160 170 180 190 200 210 m038.pep ASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVEX I I I I II I II I II I II II II II I I II I II II II I I a038 ASLNDLFILLQNNPEFGQFLEPVPAYRRQYGVEX 190 200 210 Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 038 shows 98.1% identity over a 213 aa overlap with a predicted ORF (ORF 038.ng) from N gonorrhoeae: m038/g038 10 20 30 40 50 60 m038. pep MTDFRQDFLKFSLAQΝVLKFGEFTTKAGRRSPYFFΝAGLFΝDGLSTLQLAKFYAQSIIES II II I II II I II I II II I I II I I II I II II I II I II II II II I I I I I I I II I I II II II g038 MTDFRQDFLKFSLAQΝVLKFGEFTTKAGRRSPYFFΝAGLFΝDGASTLQLAKFYAQSIIES 10 20 30 40 50 60 70 80 90 100 110 120 m038.pep GIRFDMLFGPAYKGIILAAATAMMLAEKGVΝVPFAYΝRKEAKDHGEGGVLVGAPLKGRVL I II I I I II I II I I I I I I I I I II I II I II II II I I I II I I I I II : I II II I II II II I II I g038 GIRFDMLFGPAYKGIILAAATAMMLAEKGVΝVPFAYΝRKEAKDRGEGGVLVGAPLKGRVL 70 80 90 100 110 120 130 140 150 160 170 180 m038.pep IIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGTGELSAVQEVEKQYGLPVAPI I I I II I II I I I II II II I I I II I II I I I II II II II I I II I : I II II I I II II I I II I I I g038 IIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGTGKLSAVQEVEKQYGLPVAPI 130 140 150 160 170 180 190 200 210 m038.pep ASLΝDLFILLQΝΝPEFGQFLEPVRAYRRQYGVEX I I I II II I I II II I II II II II I I : I I II I II II g038 ASLΝDLFILLQΝΝPEFGQFLEPVRTYRRQYGVEX 190 200 210 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 141>: g039.seq 1 ATGCCGTCCG AACCACCTGC CGCTTCAGAC GGCATCAAAC CGACACACAC 51 CGAGAAAACA TCATGCCCGC CTGTTTCTGT CCGCACTGCA AAACCCGCCT 101 CTGGGTCAAA GAAAcccagC TCAAcgtCgC ccaagGCTTC GTCGTCTgcc 151 aaAAAtgcga agGGCTgttt aaAgccaaaG accAtctggc aaGcacGAAA 201 gaacctatat tcaacgattg gcccgaagct gtttcgggat gTcaaaCTCG 251 TCcaccgcaT cggcacgcac gccattagca aGAaacagat gtcccgcgac 301 gaaatCgccg atatcctcaa cggcggtaca acCCTGCACG ATACGCCGCC 351 CGCAACCGCC GCTGCCGCac ctGCCGCCGC ACCGCaggTT TCCGTACCGC 401 CCGCCCGTCA GGAAGGGCTC AACTGGACTA TTGCAACCCT GTTCGCACTT 451 ATCGTCCTCA TTATGCAGCT TTCCTACCTC TTCATCCTAT GA This corresponds to the amino acid sequence <SEQ ID 142; ORF 039.ng>: g039.pep 1 MPSEPPAASD GIKPTHTEKT SCPPVSVRTA KPASGSKKPS STSPKASSSA 51 KNAKGCLKPK TIWQARKNLY STIGPKLFRD VKLVHRIGTH AISKKQMSRD 101 EIADILNGGT TLHDTPPATA AAAPAAAPQV SVPPARQEGL NWTIATLFAL 151 IVLIMQLSYL FIL* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 143>: m039.seq 1 ATGCCGTCCG AACCGCCTTA CGCCTCAGAC GGCATCAAAC CTGACACACA 51 CGAGGAAATA CCATGCCCGC CTGTTTCTGC CCCCACTGCA AAACCCGTCT 101 CTGGGTCAAA GAAACCCAAC TCAATGTCGC CGnnnnnnnn nnnnnnnnnn 151 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnCCC GAGGCTGTTT 251 CGGATGTCAA ACTCGTTCAC CGTATCGGCA CGCGCGCCAT CGGCAAGAAA 301 CAGATTTCCC GTGACGAAAT CGCCGGCATC CTCAACGGCG GTACAACCCA 351 GCCCGATATT CCGCCCGCAA CCGCCGCCAC CCCTGCTGCC GCACCGCAGG 401 TTACCGTACC GCCCGCCGCG CCCGCCCGTC AGGATGGGTT CAACTGGACG 451 ATTGCAACCC TGTTTGCCCT TATCGTCCTC ATTATGCAGC TTTCCTACCT 501 CGTCATCCTA TGA This corresponds to the amino acid sequence <SEQ ID 144; ORF 039>: m039.pep 1 MPSEPPYASD GIKPDTHEEI PCPPVSAPTA KPVSGSKKPΝ SMSPXXXXXX 51 XXXXXXXXXX XXXXXXXXXX XXXXXXXXXP EAVSDVKLVH RIGTRAIGKK 101 QISRDEIAGI LΝGGTTQPDI PPATAATPAA APQVTVPPAA PARQDGFΝWT 151 IATLFALIVL IMQLSYLVIL * The following partial DΝA sequence was identified in N meningitidis <SEQ ID 145>: a039. seq 1 ATGCCGTCTG AACCGCCTTA CGCCTCAGAC GGCATCAAAC CTGACACACA 51 CGAGGAAATA CCATGCCCGC CTGTTTCTGC CCCCACTGCA AAACCCGTCT 101 CTGGGTCAAA GAAACCCAAC TCAATGTCGC CCAAGGCTTC GTCGTCTGCC 151 AAAAATGCGA AGGAATGTTT AAAGCCAAAG ACCATCTGGC AAGCACGAAA 201 GAACCCATAT TCAACGATT . TGCCCGAAGC TGTTTCGGAT GTCAAACTCG 251 TTCACCGCAT CGGCACGAGC GCCATCGGCA AGAAACAGAT TTCCCGTGAC 301 GAAATCGCCG GCATCCTCAA CGGCGGCACA ACCCAGCCCG ATATTCCGCC 351 CGCAACCGCC GCCACCCCTG CTGCCGCACC GCAGGTTACC GTACCGCCCG 401 CCGCGCCCGC CCGTCAGGAT GGGTTCAACT GGACGATTGC AACCCTGTTT 451 GCCCTTATCG TCCTCATTAT GCAGCTTTCC TACCTCGTCA TCCTATGA This corresponds to the amino acid sequence <SEQ ID 146; ORF 039. a>: a039.pep 1 MPSEPPYASD GIKPDTHEEI PCPPVSAPTA KPVSGSKKPΝ SMSPKASSSA 51 KΝAKECLKPK TIWQARKΝPY STIXPEAVSD VKLVHRIGTS AIGKKQISRD 101 EIAGILΝGGT TQPDIPPATA ATPAAAPQVT VPPAAPARQD GFΝWTIATLF 151 ALIVLIMQLS YLVIL* 039/a039 19.4% identity over a 170 aa overlap 10 20 30 40 50 60 m039.pep MPSEPPYASDGIKPDTHEEIPCPPVSAPTAKPVSGSKKPΝSMSPXXXXXXXXXXXXXXXX II II I I II I I II I I II I II II I I II I I I II II II II II II II I I a039 MPSEPPYASDGIKPDTHEEIPCPPVSAPTAKPVSGSKKPΝSMSPKASSSAKΝAKECLKPK 10 20 30 40 50 60 70 80 90 100 110 120 m039.pep XXXXXXXXXXXXXXXXXXXPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTQPDI : : I I I I I II I I I II I I II I II I I I II II II II I I I II I II II I a039 TIWQARKNPYSTIX PEAVSDVKLVHRIGTSAIGKKQISRDEIAGILNGGTTQPDI 70 80 90 100 110 130 140 150 160 170 m039.pep PPATAATPAAAPQVTVPPAAPARQDGFNWTIATLFALIVLIMQLSYLVILX II II I II II II I II I I II I II II I I I II I II II I II I I I II I II II I I II I a039 PPATAATPAAAPQVTVPPAAPARQDGFNWTIATLFALIVLIMQLSYLVILX 120 130 140 150 160 Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 039 shows 60.8% identity over a 171 aa overlap with a predicted ORF (ORF 039.ng) from N. gonorrhoeae: m039/g039 10 20 30 40 50 60 m039. pep MPSEPPYASDGIKPDTHEEIPCPPVSAPTAKPVSGSKKPΝSMSPXXXXXXXXXXXXXXXX h llllh lllhlllllhl II g039 MPSEPPAASDGIKPTHTEKTSCPPVSVRTAKPASGSKKPSSTSPKASSSAKΝAKGCLKPK 10 20 30 40 50 60 70 80 90 100 110 120 m039. pep XXXXXXXXXXXXXXXXXXXPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILΝGGTTQPDI h I g039 TIWQARKΝLYSTIG PKLFRDVKLVHRIGTHAISKKQMSRDEIADILΝGGTTLHDT 70 80 90 100 110 130 140 150 160 170 m039. pep PPATAAT-PAAAPQVTVPPAAPARQDGFΝWTIATLFALIVLIMQLSYLVILX l l l l l h I I I g039 PPATAAAAPAAAPQVSVPPA RQEGLΝWTIATLFALIVLIMQLSYLFILX 120 130 140 150 160 The following partial DΝA sequence was identified in N. gonorrhoeae <SEQ ID 147>: g040. seq 1 ATGAACGCGC CCGACAGCTT TGTCGCCCAC TTCCGCGAAG CCGCCCCCTA 51 CATCCGCCAA ATGCGCGGCA CGACACTGGT CGCCGGCATA GAcggCCGCC 101 TGCTCGAAGG CGGCACCTTA AATAAGCTCG CCGCCGACAT CGGGCTGTTG 151 TCGCAACTGG GCATCCGACT CGTCCTCATC CACGGCGCGT ACCACTTCCT 201 CGAccgCCTC GCCGCCGCGC AAGgccGCAC GCCGCATTAT TGCCGgggtt 251 tGCGCGTTAC CGACGaAACc tcGctcgGAC AGGCGCAGCA GtttGCCGGC 301 AccgTCCGCA GCCGTTTTGA agcCGCATTG tgcggcagCG tttcaggatt 351 cgcgCGCGCG CCTTCCGTCC CGCTCGTAtc gggcaacttc ctgacCGCCC 401 GTCcgatggg cgtgattgac ggaACCGata tggaatacgc gggggttatc 451 cgcaaaaccg ACACCGCCGC CCTCCGTTTC CAACTCGACG CGGGCAATAT 501 CGTCTGGATG CCGCCGCTCG GGCATTCCTA CGGCGGCAAA ACCTTCAATC 551 TCGATATGGT GCAGGCCGCC GCTTCCGTCG CCGTCTCGCT TCAGGCCGAA 601 AAACTCGTTT ACCTGACCCT TTCAGACGGC ATTTCCCGCC CCGACGGCAC 651 GCTCGCCGAA ACCCTCTCGG CACAGGAAGC GCAATCGCTG GCGGAACACG 701 CCGCCAGCGA AACCCGACGA CTGATTTCGT CCGCCGTTGC CGCGCTCGAA 751 GGCGGCGTGC ATCGCGTCCA AATCCTCAAC GGGGCCGCCG ACGGCAGCCT 801 GCTGCAAGAA CTCTTCACCC GCAACGGCAT CGGCACGTCC ATTGCCAAAG 851 AAGCCTTCGT CTCCATCCGG CAGGCGCACA GCGGCGACAT CCCGCACATC 901 GCCGCCCTCA TCCGCCCGCT GGAAGAACAG GGCGTCCTAT TGCACCGCAG 951 CCGCGAATAC CTCGAAAACC ACATTTCCGA ATTTTCCATC CTCGAACACG 1001 ACGGCGACCT GTACGGCTGT GCCGCACTCA AAACCTTTGC CGAAGCCGAT 1051 TGCGGCGAAA TCGCCTGCCT TGCCGTCTCG CCGCAGGCAC AGGACGGCGg 1101 ctACGGCGAA CGCCTGCTTG CCCACATTAT CGATAAGGCG CGCGGCATAG 1151 GCATAAGCAG GCTGTTCGCA CTGTCCACAA ATACCGGCGA ATGGTTTGCC 1201 GAACGCGGCT TTCAGACGGC ATCGGAAGAC GAGCTGCCCG AAACGCGGCG 1251 CAAAGACTAC CGCAGCAACG GACGAAACCC GCATATTCTG GTGCGTCGCC 1301 TGCACCGCTG A This corresponds to the amino acid sequence <SEQ ID 148; ORF 040.ng>: g040.pep 1 MNAPDSFVAH FREAAPYIRQ MRGTTLVAGI DGRLLEGGTL NKLAADIGLL 51 SQLGIRLVLI HGAYHFLDRL AAAQGRTPHY CRGLRVTDET SLGQAQQFAG 101 TVRSRFEAAL CGSVSGFARA PSVPLVSGNF LTARPMGVID GTDMEYAGVI 151 RKTDTAALRF QLDAGNIVWM PPLGHSYGGK TFNLDMVQAA ASVAVSLQAE 201 KLVYLTLSDG ISRPDGTLAE TLSAQEAQSL AEHAASETRR LISSAVAALE 251 GGVHRVQILN GAADGSLLQE LFTRNGIGTS IAKEAFVSIR QAHSGDIPHI 301 AALIRPLEEQ GVLLHRSREY LENHISEFSI LEHDGDLYGC AALKTFAEAD 351 CGEIACLAVS PQAQDGGYGE RLLAHIIDKA RGIGISRLFA LSTNTGEWFA 401 ERGFQTASED ELPETRRKDY RSNGRNPHIL VRRLHR* The following partial DNA sequence was identified in N meningitidis <SEQ ID 149>: m040. seq 1 ATGAGCGCGC CCGACCTCTT TGTCGCCCAC TTCCGCGAAG CCGTCCCCTA 51 CATCCGCCAA ATGCGCGGCA AAACGCTGGT CGCCGGCATA GACGACCGCC 101 TGCTCGAAGG TGATACCTTA AACAAGCTCG CCGCCGACAT CGGGCTGTTG 151 TCGCAACTGG GCATCAGGCT CGTCCTCATC CACGGCGCGC GCCACTTCCT 201 CGACCGCCAC GCCGCCGCTC AAGGCCGCAC GCCGCATTAT TGCCGGGGCT 251 TGCGCGTTAC CGACGAAACC TCGCTCGAAC AGGCGCAgCA GTTTGCCGGC 301 ACCGTCCGCA GCCGTTTTGA AGCCGCATTG TGCGGCAGCG TTTCCGGGTT 351 CGCGCGCGCG CCTTCCGTCC CGCTCGTATC GGGCAACTTC CTGACCGCCC 401 GTCCGATAGG TGTGATTGAC GGAACCGATA TGGAATACGC GGGCGTTATC 451 CGCAAAACCG ACACCGCCGC CCTCCGTTTC CAACTCGACG CGGGCAATAT 501 CGTCTGGCTG CCGCCGCTCG GACATTCCTA CAGCGGCAAG ACCTTCTATC 551 TCGATATGCT TCAAACCGCC GCCTCCGCCG CCGTCTCGCT TCAGGCCGAA 601 AAACTCGTTT ACCTGACCCT TTCAGACGGC ATTTCCCGCC CCGACGGCAC 651 GCTCGCCGAA ACCCTCTCGG CACAGGAAGC GCAATCGCTG GCGGAACACG 701 CCGGCGGGCA AACGCGACGG CTGATTTCGT CCGCCGAACT CTTCACCCGC 751 AACGGCATCG GCACGTCCAT TGCCAAAGAA GCCTTCGTCT CCATCCGGCA 801 rGCGCAy gG G.CGACATCC CGCACATCGC CGCCCTCATC CGCCCGCTGG 851 AAGAACAGGG CATCCTGCTG CACCGCAs . c GCGAATACCT CGAAAACCAC 901 ATTTCCGAAT TTTCCATCCT CGAACACGAC GGCAACCTGT ACGGTTGCGC 951 CGCCCTGAAA ACCTTTGCCG AAGCCGATTG CGGCGAAATC GCCTGCCTTG 1001 CCGTCTCGCC GCag.cACAG GACGGCGGCT ACGGCGAACG CnTGCTTGCC 1051 CACATTATCG ATAAGGCGCG CGGCATAGGC ATAAGCAGGC TGTTCGCACT 1101 GTCCACAAAT ACCGGCGAAT GGTTTGCCGA ACGCGGCTTT CAGACGGCAT 1151 CGGAAGACGA GTTGCCCGAA ACGCGGCGCA AAGACTACCG CAGCAACGGA 1201 CGGAACTCGC ATATTCTGGT ACGTCGCCTG CACCGCTGA This corresponds to the amino acid sequence <SEQ ID 150; ORF 040>: m040.pep 1 MSAPDLFVAH FREAVPYIRQ MRGKTLVAGI DDRLLEGDTL NKLAADIGLL 51 SQLGIRLVLI HGARHFLDRH AAAQGRTPHY CRGLRVTDET SLEQAQQFAG 101 TVRSRFEAAL CGSVSGFARA PSVPLVSGNF LTARPIGVID GTDMEYAGVI 151 RKTDTAALRF QLDAGNIVWL PPLGHSYSGK TFYLDMLQTA ASAAVSLQAE 201 KLVYLTLSDG ISRPDGTLAE TLSAQEAQSL AEHAGGQTRR LISSAELFTR 251 NGIGTSIAKE AFVSIRQAHX XDIPHIAALI RPLEEQGILL HRXREYLENH 301 ISEFSILEHD GNLYGCAALK TFAEADCGEI ACLAVSPQXQ DGGYGERXLA 351 HIIDKARGIG ISRLFALSTN TGEWFAERGF QTASEDELPE TRRKDYRSNG 401 RNSHILVRRL HR* The following partial DNA sequence was identified in N meningitidis <SEQ ID 151>: a040 . seq 1 ATGATCGTGC CCGACCTCTT TGTCGCCCAC TTCCGCGAAG CCGCCCCCTA 51 CATCCGCCAA ATGCGCGGCA AAACGCTGGT CGCCGGCATA GACGACCGCC 101 TGCTCGAAGG TGATACCTTA AACAAGTTCG CCGCCGACAT CGGGCTTTTG 151 TCGCAACTGG GCATCAGGCT CGTCCTCATC CACGGCGCGC GCCACTTCCT 201 CGACCGCCAC GCCGCCGCGC AAGGCCGCAC GCCGCATTAT TGCCGGGGCT 251 TGCGCGTTAC CGACGAAACC TCGCTCGAAC AGGCGCAGCA GTTTGCCGGC 301 ACCGTCCGCA GCCGTTTTGA AGCCGCATTG TGCGGCAGCG TTTCCGGGTT 351 CGCGCGCGCG CCTTCCGTCC CGCTCGTATC GGGCAACTTC CTGACCGCCC 401 GTCCGATAGG TGTGATTGAC GGAACCGATA TGGAATACGC GGGCGTTATC 451 CGCAAAACCG ACACCGCCGC CCTCCGTTTC CAACTCGACG CGGGCAATAT 501 CGTCTGGCTG CCGCCGCTCG GACATTCCTA CAGCGGCAAG ACCTTCCATC 551 TCGATATGCT TCAAACCGCC GCCTCCGTCG CCGTCTCGCT TCAGGCCGAA 601 AAACTCGTTT ACCTGACCCT TTCAGACGGC ATTTCCCGCC CCGACGGCAC 651 GCTCGCCGTA ACCCTCTCGG CACAGGAAGC GCAATCGCTG GCGGAACACG 701 CCGGCGGCGA AACGCGACGG CTGATTTCGT CCGCCGTTGC CGCGCTCGAA 751 GGCGGCGTGC ATCGCGTCCA AATCCTCAAC GGAGCCGCCG ACGGCAGCCT 801 GCTGCAAGAA CTCTTCACCC GCAACGGCAT CGGCACGTCC ATTGCCAAAG 851 AAGCCTTCGT CTCCATCCGG CAGGCGCACA GCGGCGACAT CCCGCACATT 901 GCCGCCCTCA TCCGCCCGCT GGAAGAACAG GGCATCCTGC TGCACCGCAG 951 CCGCGAATAC CTCGAAAACC ACATTTCCGA ATTTTCCATC CTCGAACACG 1001 ACGGCAACCT GTACGGTTGC GCCGCCCTGA AAACCTTTGC CGAAGCCGAT 1051 TGCGGCGAAA TCGCCTGCCT TGCCGTCTCG CCGCAGGCAC AGGACGGCGG 1101 CTACGGCGAA CGCCTGCTTG CCCACATTAT CGATAAGGCG CGCGGCATAG 1151 GCATAAGCAG GCTGTTCGCA CTGTCCACAA ATACCGGCGA ATGGTTTGCC 1201 GAACGCGGCT TTCAGACGGC ATCGGAAGAC GAGTTGCCCG AAACGCGGCG 1251 CAAAGACTAC CGCAGCAACG GACGGAACTC GCATATTCTG GTGCGTCGCC 1301 TGCACCGCTG A This corresponds to the amino acid sequence <SEQ ID 152; ORF 040. a>: a040.pep 1 MIVPDLFVAH FREAAPYIRQ MRGKTLVAGI DDRLLEGDTL NKFAADIGLL 51 SQLGIRLVLI HGARHFLDRH AAAQGRTPHY CRGLRVTDET SLEQAQQFAG 101 TVRSRFEAAL CGSVSGFARA PSVPLVSGNF LTARPIGVID GTDMEYAGVI 151 RKTDTAALRF QLDAGNIVWL PPLGHSYSGK TFHLDMLQTA ASVAVSLQAE 201 KLVYLTLSDG ISRPDGTLAV TLSAQEAQSL AEHAGGETRR LISSAVAALE 251 GGVHRVQILN GAADGSLLQE LFTRNGIGTS IAKEAFVSIR QAHSGDIPHI 301 AALIRPLEEQ GILLHRSREY LENHISEFSI LEHDGNLYGC AALKTFAEAD 351 CGEIACLAVS PQAQDGGYGE RLLAHIIDKA RGIGISRLFA LSTNTGEWFA 401 ERGFQTASED ELPETRRKDY RSNGRNSHIL VRRLHR* m040/a040 91.5% identity in 436 aa overlap 10 20 30 40 50 60 m040.pep MSAPDLFVAHFREAVPYIRQMRGKTLVAGIDDRLLEGDTLNKLAADIGLLSQLGIRLVLI I : I II II II II II : I II I II II I II I II II II I II II II II : II II I II I I II I II I II a040 MIVPDLFVAHFREAAPYIRQMRGKTLVAGIDDRLLEGDTLNKFAADIGLLSQLGIRLVLI 10 - 20 30 40 50 60 70 80 90 100 110 120 m040.pep HGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTVRSRFEAALCGSVSGFARA II I I I II II II II I I II II I II II II II II II I II II I I I I II II I II II II II II II I I a040 HGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTVRSRFEAALCGSVSGFARA 70 80 90 100 110 120 130 140 150 160 170 180 m040.pep PSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRFQLDAGNIVWLPPLGHSYSGK II II II II I I II II II I I I II II I I II II II II I II II II II II I II II I II I II I II II a040 PSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRFQLDAGNIVWLPPLGHSYSGK 130 140 150 160 170 180 190 200 210 220 230 240 m040.pep TFYLDMLQTAASAAVSLQAEKLVYLTLSDGISRPDGTLAETLSAQEAQSLAEHAGGQTRR II : I I II I I II I : I I I II II II II I I I I II I I II I II II I II II II II II II M I : I II a040 TFHLDMLQTAASVAVSLQAEKLVYLTLSDGISRPDGTLAVTLSAQEAQSLAEHAGGETRR 190 200 210 220 230 240 250 260 270 m040.pep LISSA ELFTRNGIGTSIAKEAFVSIRQAHXXDIPHI II II I I II II II II II I II I II I II I II I I II I I a0 0 LISSAVAALEGGVHRVQILNGAADGSLLQELFTRNGIGTSIAKEAFVSIRQAHSGDIPHI 250 260 270 280 290 300 280 290 300 310 320 330 m040.pep AALIRPLEEQGILLHRXREYLENHISEFSILEHDGNLYGCAALKTFAEADCGEIACLAVS I II II II II I II I II I I II II II I I II I II I I I I II II II II II II I I II II II I II II a040 AALIRPLEEQGILLHRSREYLENHISEFSILEHDGNLYGCAALKTFAEADCGEIACLAVS 310 320 330 340 350 360 340 350 360 370 380 390 m040.pep PQXQDGGYGERXLAHIIDKARGIGISRLFALSTNTGEWFAERGFQTASEDELPETRRKDY II II II II II I II I I II I II II II II I I II II I II II II I II I II I I II II I II II II a040 PQAQDGGYGERLLAHIIDKARGIGISRLFALSTNTGEWFAERGFQTASEDELPETRRKDY 370 380 390 400 410 420 400 410 m040.pep RSNGRNSHILVRRLHRX I I I II II II II I I I I II a040 RSNGRNSHILVRRLHRX 430 Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 040 shows 88.3% identity over a 436 aa overlap with a predicted ORF (ORF 040.ng) from N gonorrhoeae: m040/g040 m040.pep MSAPDLFVAHFREAVPYIRQMRGKTLVAGIDDRLLEGDTLΝKLAADIGLLSQLGIRLVLI 60 1:111 II I II I I I : II II I I I I lllllll I II II I I II II II I II I II II I II II I g040 MΝAPDSFVAHFREAAPYIRQMRGTTLVAGIDGRLLEGGTLΝKLAADIGLLSQLGIRLVLI 60 m040.pep HGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTVRSRFEAALCGSVSGFARA 120 III I II II II I I I I I II I I II II I II I I I I I II II I I II II II I II I II II I II II I g040 HGAYHFLDRLAAAQGRTPHYCRGLRVTDETSLGQAQQFAGTVRSRFEAALCGSVSGFARA 120 m040.pep PSVPLVSGΝFLTARPIGVIDGTDMEYAGVIRKTDTAALRFQLDAGΝIVWLPPLGHSYSGK 180 II II II I I I I I II I I : I I II I II I II II I II II I II II II II II II II I : II II II I : I I g040 PSVPLVSGΝFLTARPMGVIDGTDMEYAGVIRKTDTAALRFQLDAGΝIVWMPPLGHSYGGK 180 m040.pep TFYLDMLQTAASAAVSLQAEKLVYLTLSDGISRPDGTLAETLSAQEAQSLAEHAGGQTRR 240 II I II : I : I I I : I II II II II I I II II I II II I II I I I II I I M I II II I II I ::: I II g040 TFΝLDMVQAAASVAVSLQAEKLVYLTLSDGISRPDGTLAETLSAQEAQSLAEHAASETRR 240 m040.pep LISSA ELFTRΝGIGTSIAKEAFVSIRQAHXXDIPHI 276 II II I II II I II II II I I I I I II I I II II I II II g040 LISSAVAALEGGVHRVQILΝGAADGSLLQELFTRΝGIGTSIAKEAFVSIRQAHSGDIPHI 300 m040.pep AALIRPLEEQGILLHRXREYLEΝHISEFSILEHDGΝLYGCAALKTFAEADCGEIACLAVS 336 II II II II II I: II II II I II I I II II I II I II I : I I II I I I II I II II II I II II I II g040 AALIRPLEEQGVLLHRSREYLEΝHIΞEFSILEHDGDLYGCAALKTFAEADCGEIACLAVS 360 m040.pep PQXQDGGYGERXLAHIIDKARGIGISRLFALSTΝTGEWFAERGFQTASEDELPETRRKDY 396 II II II II II I I II II I I II II I I I I II I I II I II II I II II II I II II I II II II I I g040 PQAQDGGYGERLLAHIIDKARGIGISRLFALSTΝTGEWFAERGFQTASEDELPETRRKDY 420 m040.pep RSNGRNSHILVRRLHRX 413 llllll I II I I I I II I g040 RSNGRNPHILVRRLHRX 437 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 153>: g041. seq 1 ATGAGTTCGC CCAAACACAT CGGCTTGCAG GGCGGCAGCA ACGGCGGCCT 51 GATTACCGCC GCCGCCTTCG TGCGCGAACC GCAAAGCATC GGTGCGCTGG 101 TGTGCGAAGT ACCGCTGACC GATATGATCC GTTATCCGCT GCTGTCCGCC 151 GGTTCAAGTT GGACGGACGA ATACGGCAAT CCGCAGAAAT ACGAAGCCTG 201 CAAACGCCGG CTGGGCGAAT TGTCGCCGTA TCACAATCTT TCAGACGGCA 251 TCGATTATCC GCCCGCACTC ATTACCACCA GCCTCAGCGA CGACCGCGTC 301 CATCCCGCCC ACGCGCTCAA ATTCTACGCC AAACTGCGCG AAACCTCGCC 351 GCAATCTTGG CTCTACTCGC CTGACGGCGG CGGCCATACC GGCAACGGCA 401 CCCAACGCGA ATCCGCCGAC AAACTCGCCT GCGTGTTGCT GTTTTTGAAA 451 GAATTTTTGG GATAA This corresponds to the amino acid sequence <SEQ ID 154; ORF 041. ng>: g041.pep 1 MΞSPKHIGLQ GGSΝGGLITA AAFVREPQSI GALVCEVPLT DMIRYPLLSA 51 GSSWTDEYGΝ PQKYEACKRR LGELSPYHΝL SDGIDYPPAL ITTSLSDDRV 101 HPAHALKFYA KLRETSPQSW LYSPDGGGHT GΝGTQRESAD KLACVLLFLK 151 EFLG* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 155>: m041. seq 1 ATCAGTTCGC CCGAACACAT CGGCTTGCAG GGCGGCAGCA ACGGCGGACT 51 GATTACTGCC GCCGCCTTCG TGCGCGAACC GCAAAGCATC GGCGCGCTGG 101 TGTGCGAAGT GCCGCTGACC GACATGATCC GTTATCCGCT GCTCTCCGCC 151 GGTTCAAGCT GGACAGACGA ATACGGCAAT CCGCAAAAAT ACGAAGTCTG 201 CAAACGCCGG TTGGGCGAAT TGTCGCCGTA TCACAATCTT TCAGACGGCA 251 TCGATTATCC GCCCGCGCTC ATTACCACCA GCCTGTCCGA CGATCGCGTC 301 CATCCCGCCC ACGCGCTCAA GTTCTACGCC AAACTGCGCG AAACCTCCGC 351 GCAATCTTGG CTCTACTCGC CTGACGGCGG CGGCCATACC GGCAACGGCA 401 CCCAACGCGA ATCCGCCGAC GAACTCGCCT GCGTCTTGCT GTTTTTGAAA 451 GAGTTTTTGG GCTAA This corresponds to the amino acid sequence <SEQ ID 156; ORF 041>: m041.pep 1 ISSPEHIGLQ GGSΝGGLITA AAFVREPQSI GALVCEVPLT DMIRYPLLSA 51 GSSWTDEYGΝ PQKYEVCKRR LGELSPYHΝL SDGIDYPPAL ITTSLSDDRV 101 HPAHALKFYA KLRETSAQSW LYSPDGGGHT GΝGTQRESAD ELACVLLFLK 151 EFLG* The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 157>: a041. seq 1 ATCAGTTCGC CCGAACACAT CGGCTTGCAG GGCGGCAGCA ACGGCGGACT 51 GATTACTGCC GCCGCCTTCG TGCGCGAACC GCAAAGCATA GGCGCGCTGG 101 TGTGCGAAGT GCCGCTGACC GACATGATCC GTTATCCGCT GCTCTCCGCC 151 GGTTCAAGCT GGACAGACGA ATACGGCAAT CCGCAAAAAT ACGAAGTCTG 201 CAAACGCCGG TTGGGCGAAT TGTCGCCGTA TCACAATCTT TCAGACGGCA 251 TCGATTATCC GCCCGCGCTC ATTACCACCA GCCTGTCCGA CGATCGCGTC 301 CATCCCGCCC ACGCGCTCAA GTTCTACGCC AAACTGCGCG AAACCTCGCC 351 GCAATCTTGG CTCTACTCGC CTGACGGCGG CGGCCATACC GGCAACGGCA 401 CGCAGCGCGA AGCCGCCGAC GAACTCGCCT GCGTGTTGCT GTTTTTGAAA 451 GAGTTTTTGG GCTAA This corresponds to the amino acid sequence <SEQ ID 158; ORF 041. a>: a041.pep 1 ISSPEHIGLQ GGSΝGGLITA AAFVREPQSI GALVCEVPLT DMIRYPLLSA 51 GSSWTDEYGΝ PQKYEVCKRR LGELSPYHΝL SDGIDYPPAL ITTSLSDDRV 101 HPAHALKFYA KLRETSPQSW LYSPDGGGHT GΝGTQREAAD ELACVLLFLK 151 EFLG* m04i/a04i 98.7%> identity over a 154 aa overlap 10 20 30 40 50 60 m041.pep ISSPEHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLSAGSSWTDEYGN II II II II I II II II I II II I II II II I I I I II I I II II II II I II I II I I I II I I II II a041 ISSPEHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLSAGSSWTDEYGN 10 20 30 40 50 60 70 80 90 100 110 120 m041.pep PQKYEVCKRRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALKFYAKLRETSAQSW II II I I I II II I II II I II II II I II I I II II I I II II II II I I II II I I I II I I I III a041 PQKYEVCKRRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALKFYAKLRETSPQSW 70 80 90 100 110 120 130 140 150 m041.pep LYSPDGGGHTGNGTQRESADELACVLLFLKEFLGX I II II II I II II II I I I : II II II I II I II I I I I I a041 LYSPDGGGHTGNGTQREAADELACVLLFLKEFLGX 130 140 150 Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 041 shows 96.8% identity over a 154 aa overlap with a predicted ORF (ORF 041.ng) from N gonorrhoeae: m041/g041 10 20 30 40 50 60 m041.pep ISSPEHIGLQGGSΝGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLSAGSSWTDEYGΝ : II I : II II I II II I I I I I I I II I I II I II II I II II II I II II I II I I I I I I I II II I I g041 MSSPKHIGLQGGSΝGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLSAGSSWTDEYGΝ 10 20 30 40 50 60 70 80 90 100 110 120 m041.pep PQKYEVCKRRLGELSPYHΝLSDGIDYPPALITTSLSDDRVHPAHALKFYAKLRETSAQSW I II II: II II II II I I II II I II I I I II I I II I I II I II I II I I I II II II II I I I III g041 PQKYEACKRRLGELSPYHΝLSDGIDYPPALITTSLSDDRVHPAHALKFYAKLRETSPQSW 70 80 90 100 110 120 130 140 150 mO41. pep LYSPDGGGHTGΝGTQRESADELACVLLFLKEFLGX I II II I II II II II I I I I I I : I I I I II I I I II I II g041 LYSPDGGGHTGΝGTQRESADKLACVLLFLKEFLGX 130 140 150 The following partial DΝA sequence was identified in N. gonorrhoeae <SEQ ID 159>: g041-l.seq 1 ATGAAATCCT ACCCCGACCC CTACCGCCAT TTTGAAAACC TCGATTCCGC 51 CGAAACGCAA AACTTCGCTG CTGAAGCGAA TGCCGAAACG CGCGCGCGTT 101 TTTTAAACAA CGACAAGGCG CGCGCACTTT CAGACGGCAT TTTGAATCAA 151 ATGCAGGACA CGCGGCAGAT TCCGTTTTGT CAGGAACACC GCGCGCGGAT 201 GTACCATTTC CATCAGAATG CGGAATATCC GAAGGGCGTG TACCGCATGT 251 GTACGGCGGC GACCTACCGT TCCGGCTATC CCGAGTGGAA AATCCTGTTT 301 TCGGTGGCGG ATTTCGATGA GTTGCTCGGC GACGATGTGT ATTTGGGCGG 351 CGTGTCGCAC TTGGTGGAGC AGCCCAACCG CGCGCTGCTG ACTTTGAACA 401 AATCGGGCGG CGATACGGCG TATACGCTGG AAGTGGATTT GGAAGCAGGG 451 GAATTGGTAG AGGGCGGTTT TCACTTTCCG GCAGGCAAAA ACCATGTGTC 501 GTGGCGCGAT GAAAACAGCG TGTGGGTGTG TCCGGCTTGG GACGAACGCC 551 AGTTGACCGA ATCGGGCTAT CCGCGCGAAG TGTGGCTGGT GGAACGCGGC 601 AAGAGTTTCG AGGAAAGCCT GCCGGCGTAC CAAATCGATA AAGGCGCGAT 651 GATGGTAAAC GCGTGGCGTT ACCTCGATCC GCAGGGTTCG CCGATTGATT 701 TGATTGAAGC GTCGGACGGT TTTTACACCA AGACGTATTT GCAGGTGTCG 751 TCCGAAGGCG GGGCGAAACC GTTGAACCTG CCTAATGATT GCGATGTGGT 801 CGGCTATCTG GCGGGACATC TTTTGCTGAC GCTGCGCAAG GACTGGCACC 851 GCGCGAACCA AAGCTATCCG AGTGGCGCGT TGGTGGCGGT GAAACTGAAT 901 CGGGGCGAAC TCGGGGCGGC GCAGCTTTTG TTTGCGCCCG ATGAAACGCA 951 GGCATTGGAA AGCGTGGAAA CGACCAAGCG TTTTGTGGTG GCAAGCCTGC 1001 TGGAGAATGT ACAAGGCCGT CTGAAAGCGT GGCGGTTTGC CGACAGCAAA 1051 TGGCAGGAAG CCGAGTTGCC GCACCTGCCC TCGGGCGCGT TGGAAATGAC 1101 CGACCAACCG TGGGGCGGCG ACGTGGTTTA TCTTGCCGCC AGCGATTTCA 1151 CCACGCCGCT GACGCTGTTT GCGCTGGATT TGAACGTGAT GGAACTGACC 1201 GTCATGCGCC TCCAGCCGCA GCAGTTTGTT TCAGACGGCA TCGAAGTGCG 1251 GCAGTTTTGG GCGGTGTCGT CCGACGGCGA ACGCATTCCT TATTTCCACG 1301 TCGGCAAAAA CGCCGCGCCC GACACGCCGA CCTTAGTCTA TGCTTACGGA 1351 GGTTTCGGCA TTCCTGAATT GCCGCATTAT CTGGGCAGCG TCGGCAAATA 1401 TTGGCTGGAA GAGGGCAATG CCTTTGTATT GGCAAACATC CGCGGCGGCG 1451 GAGAATTCGG CCCGCGCTGG CATCAGGCGG CGCAGGGAAT CAGCAAACAC 1501 AAAAGCGTTG ATGATTTGTT GGCAGTCGTG CGTGATTTGT CCGAACGCGG 1551 CATGAGTTCG CCCAAACACA TCGGCTTGCA GGGCGGCAGC AACGGCGGCC 1601 TGATTACCGC CGCCGCCTTC GTGCGCGAAC CGCAAAGCAT CGGTGCGCTG 1651 GTGTGCGAAG TACCGCTGAC CGATATGATC CGTTATCCGC TGCTGTCCGC 1701 CGGTTCAAGT TGGACGGACG AATACGGCAA TCCGCAGAAA TACGAAGCCT 1751 GCAAACGCCG GCTGGGCGAA TTGTCGCCGT ATCACAATCT TTCAGACGGC 1801 ATCGATTATC CGCCCGCACT CATTACCACC AGCCTCAGCG ACGACCGCGT 1851 CCATCCCGCC CACGCGCTCA AATTCTACGC CAAACTGCGC GAAACCTCGC 1901 CGCAATCTTG GCTCTACTCG CCTGACGGCG GCGGCCATAC CGGCAACGGC 1951 ACCCAACGCG AATCCGCCGA CAAACTCGCC TGCGTGTTGC TGTTTTTGAA 2001 AGAATTTTTG GGATAA This corresponds to the amino acid sequence <SEQ ID 160; ORF 041-l.ng>: g041-l.pep 1 MKSYPDPYRH FENLDSAETQ NFAAEANAET RARFLNNDKA RALSDGILNQ 51 MQDTRQIPFC QEHRARMYHF HQNAEYPKGV YRMCTAATYR SGYPEWKILF 101 SVADFDELLG DDVYLGGVSH LVEQPNRALL TLNKSGGDTA YTLEVDLEAG 151 ELVEGGFHFP AGKNHVSWRD ENSVWVCPAW DERQLTESGY PREVWLVERG 201 KSFEESLPAY QIDKGAMMVN AWRYLDPQGS PIDLIEASDG FYTKTYLQVS 251 SEGGAKPLNL PNDCDWGYL AGHLLLTLRK DWHRANQSYP SGALVAVKLN 301 RGELGAAQLL FAPDETQALE SVETTKRFW ASLLENVQGR LKAWRFADSK 351 WQEAELPHLP SGALEMTDQP WGGDWYLAA SDFTTPLTLF ALDLNVMELT 401 VMRLQPQQFV SDGIEVRQFW AVSSDGERIP YFHVGKNAAP DTPTLVYAYG 451 GFGIPELPHY LGSVGKYWLE EGNAFVLANI RGGGEFGPRW HQAAQGISKH 501 KSVDDLLAVV RDLSERGMSS PKHIGLQGGS NGGLITAAAF VREPQSIGAL 551 VCEVPLTDMI RYPLLSAGSS WTDEYGNPQK YEACKRRLGE LSPYHNLSDG 601 IDYPPALITT SLSDDRVHPA HALKFYAKLR ETSPQSWLYS PDGGGHTGNG 651 TQRESADKLA CVLLFLKEFL G* The following partial DNA sequence was identified in N meningitidis <SEQ ID 161>: m041-l . seq 1 ATGAAATCCT ACCCCGACCC CTACCGCCAT TTTGAAAACC TCGATTCCGC 51 CGAAACGCAA AACTTCGCTG CTGAAGCGAA TGCCGAAACG CGCGCGCGTT 101 TTTTAGAAAA CGACAAGGCG CGCGCGCTTT CAGACGGCAT TTTGGCGCAG 151 TTGCAGGACA CGCGGCAGAT TCCGTTTTGT CAGGAACACC GCGCGCGGAT 201 GTACCATTTC CATCAGGACG CGGAGTATCC GAAGGGCGTG TACCGCGTGT 251 GTACCGCGGC GACGTATCGT TCCGGCTATC CCGAGTGGAA AATCCTGTTT 301 TCGGTGGCGG ATTTCGACGA ATTGCTTGGC GACGATGTGT ATTTGGGCGG 351 CGTGTCGCAC TTGGTGGAAC AGCCCAACCG CGCGTTGTTA ACACTGAGCA 401 AATTGGGCAG CGATACGGCG TACACGCTGG AAGTGGATTT GGAAGCAGGG 451 GAGTTGGTCG AAGGCGGTTT TCACTTTCCG GCAGGCAAAA ACCATGTGTC 501 GTGGCGCGAT GAAAACAGCG TGTGGGTGTG TCCGGCTTGG AACGAACGCC 551 AGTTGACCCA ATCGGGCTAT CCGCGCGAAG TATGGCTGGT GGAACGCGGC 601 AAGAGTTTCG AGGAAAGCCT GCCTGTGTAT CAAATCGGCG AAGACGGCAT 651 GATGGTGAAC GCGTGGCGTT ATCTCGATCC GCAGGGTTCG CCGATTGATT 701 TGATTGAAGC GTCGGACGGT TTTTACACCA AAACCTATTT GCGGGTCTCA 751 GCCGAAGGCG AGGCGAAACC GTTAAACCTG CCCAACGATT GCGACGTGGT 801 CGGCTATCTG GCGGGGCATC TTTTGCTGAC GCTGCGCAAG GACTGGAACC 851 GCGCGAACCA AAGCTATCCG AGCGGCGCGC TGGTGGCGGT GAAGCTGAAT 901 CGGGGCGAAC TCGGGGCGGC GCAGCTTTTG TTTGCGCCCG ATGAAACGCA 951 GGCATTGGAA AGCGTGGAAA CGACCAAGCG TTTTGTGGTG GCGAGCCTGT 1001 TGGAGAACGT ACAAGGCCGT CTGAAAGCAT GGCGGTTTGC CGACGGCAAA 1051 TGGCAGGAAG TCGAATTGCC GCGCCTGCCT TCGGGCGCGT TGGAAATGAC 1101 CGACCAACCT TGGGGCGGCG ACGTGGTTTA CCTTGCCGCC AGCGATTTCA 1151 CCACGCCGCT GACGCTGTTT GCGCTGGATT TGAACGTGAT GGAACTGACC 1201 GTCATGCGCC GCCAGCCGCA GCAGTTTGAT TCAGACGGCA TTAACGTGCA 1251 GCAGTTTTGG ACGACTTCGG CTGACGGCGA GCGCATTCCT TATTTCCACG 1301 TCGGCAAAAA CGCCGCGCCC GACATGCCGA CGCTGGTCTA TGCCTACGGC 1351 GGTTTCGGCA TTCCCGAATT GCCGCATTAT CTGGGCAGCA TTGGCAAATA 1401 TTGGCTGGAA GAGGGCAATG CCTTTGTATT GGCGAACATC CGCGGCGGCG 1451 GCGAGTTCGG CCCGCGCTGG CATCAGGCGG CGCAGGGAAT CAGCAAACAT 1501 AAAAGCGTTG ATGATTTATT GGCAGTCGTG CGCGATTTGT CCGAACGCGG 1551 TATCAGTTCG CCCGAACACA TCGGCTTGCA GGGCGGCAGC AACGGCGGAC 1601 TGATTACTGC CGCCGCCTTC GTGCGCGAAC CGCAAAGCAT CGGCGCGCTG 1651 GTGTGCGAAG TGCCGCTGAC CGACATGATC CGTTATCCGC TGCTCTCCGC 1701 CGGTTCAAGC TGGACAGACG AATACGGCAA TCCGCAAAAA TACGAAGTCT 1751 GCAAACGCCG GTTGGGCGAA TTGTCGCCGT ATCACAATCT TTCAGACGGC 1801 ATCGATTATC CGCCCGCGCT CATTACCACC AGCCTGTCCG ACGATCGCGT 1851 CCATCCCGCC CACGCGCTCA AGTTCTACGC CAAACTGCGC GAAACCTCCG 1901 CGCAATCTTG GCTCTACTCG CCTGACGGCG GCGGCCATAC CGGCAACGGC 1951 ACCCAACGCG AATCCGCCGA CGAACTCGCC TGCGTCTTGC TGTTTTTGAA 2001 AGAGTTTTTG GGCTAA This corresponds to the amino acid sequence <SEQ ID 162; ORF 041-1>: m041-l.pep 1 MKSYPDPYRH FENLDSAETQ NFAAEANAET RARFLENDKA RALSDGILAQ 51 LQDTRQIPFC QEHRARMYHF HQDAEYPKGV YRVCTAATYR SGYPEWKILF 101 SVADFDELLG DDVYLGGVSH LVEQPNRALL TLSKLGSDTA YTLEVDLEAG 151 ELVEGGFHFP AGKNHVSWRD ENSVWVCPAW NERQLTQSGY PREVWLVERG 201 KSFEESLPVY QIGEDGMMVN AWRYLDPQGS PIDLIEASDG FYTKTYLRVS 251 AEGEAKPLNL PNDCDWGYL AGHLLLTLRK DWNRANQSYP SGALVAVKLN 301 RGELGAAQLL FAPDETQALE SVETTKRFW ASLLENVQGR LKAWRFADGK 351 QEVELPRLP SGALEMTDQP WGGDWYLAA SDFTTPLTLF ALDLNVMELT 401 VMRRQPQQFD SDGINVQQFW TTSADGERIP YFHVGKNAAP DMPTLVYAYG 451 GFGIPELPHY LGSIGKYWLE EGNAFVLANI RGGGEFGPRW HQAAQGISKH 501 KSVDDLLAVV RDLSERGISΞ PEHIGLQGGS NGGLITAAAF VREPQSIGAL 551 VCEVPLTDMI RYPLLSAGSS WTDEYGNPQK YEVCKRRLGE LSPYHNLSDG 601 IDYPPALITT SLSDDRVHPA HALKFYAKLR ETSAQΞWLYS PDGGGHTGNG 651 TQRESADELA CVLLFLKEFL G* m041-l/g041-l 94.6% identity in 671 aa overlap 10 20 30 40 50 60 m041-l .pep MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLENDKARALSDGILAQLQDTRQIPFC II II I II II I II I I I II II I I II I II I II II II II: I I II II I I II II I : II I I I I I I I g041-l MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLNNDKARALSDGILNQMQDTRQIPFC 10 20 30 40 50 60 70 80 90 100 110 120 m0 1-l.pep QEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSVADFDELLGDDVYLGGVSH I I II II I II II I : I I I II II II : II II I I II I I I I II II II I II I I I I II II II I I II I I g041-l QEHRARMYHFHQNAEYPKGVYRMCTAATYRSGYPEWKILFSVADFDELLGDDVYLGGVSH 70 80 90 100 110 120 130 140 150 160 170 180 m041-l.pep LVEQPNRALLTLSKLGSDTAYTLEVDLEAGELVEGGFHFPAGKNHVSWRDENSVWVCPAW 111111111111:1 I : I II II I II II II II II I II II I II I II I II I I II I I I II II M g041-l LVEQPNRALLTLNKSGGDTAYTLEVDLEAGELVEGGFHFPAGKNHVSWRDENSVWVCPAW 130 140 150 160 170 180 190 200 210 220 230 240 mO41-1. pep NERQLTQSGYPREVWLVERGKSFEESLPVYQIGEDGMMVNAWRYLDPQGSPIDLIEASDG : I I I I I I I I M I I I II II I I II II I II : II I : : II II M II II II I I II II II I II I g041-l DERQLTESGYPREVWLVERGKSFEESLPAYQIDKGAMMVNAWRYLDPQGSPIDLIEASDG 190 200 210 220 230 240 250 260 270 280 290 300 mO41-1. pep FYTKTYLRVSAEGEAKPLNLPNDCDWGYLAGHLLLTLRKDWNRANQSYPSGALVAVKLN 1111111:11:11 I II II II I I II I II I I II I II II II II I : I II I II II I I II II I II g041-l FYTKTYLQVSSEGGAKPLNLPNDCDWGYLAGHLLLTLRKDWHRANQSYPSGALVAVKLN 250 260 270 280 290 300 310 320 330 340 350 360 m041-l.pep RGELGAAQLLFAPDETQALESVETTKRFVVASLLENVQGRLKAWRFADGKWQEVELPRLP I II I I II II I I I II I II II I II I II I II I I II I II II II II I II II II : I I I I : I II : II g0 1-l RGELGAAQLLFAPDETQALESVETTKRFWASLLENVQGRLKAWRFADSKWQEAELPHLP 310 320 330 340 350 360 370 380 390 400 410 420 mO41-1. pep SGALEMTDQPWGGDWYLAASDFTTPLTLFALDLNVMELTVMRRQPQQFDSDGINVQQFW I II I II II II II II I II II I II I I I I II I I II I I I II II II II I I II I I II I : I : II I g041-l SGALEMTDQPWGGDWYLAASDFTTPLTLFALDLNVMELTVMRLQPQQFVSDGIEVRQFW 370 380 390 400 410 420 430 440 450 460 470 480 m041-l.pep TTSADGERIPYFHVGKNAAPDMPTLVYAYGGFGIPELPHYLGSIGKYWLEEGNAFVLANI : : I : II II II II II I II II I I I II I II I II II I I I II II I II : I II I II II I II I II II ■ g041-l AVSSDGERIPYFHVGKNAAPDTPTLVYAYGGFGIPELPHYLGSVGKYWLEEGNAFVLANI 430 440 450 460 470 480 490 500 510 520 530 540 m041-l.pep RGGGEFGPRWHQAAQGISKHKSVDDLLAWRDLΞERGISSPEHIGLQGGSNGGLITAAAF II I I I II I I II II II II I II I I II I II II I II I I I II : I I I : I II I I I I II II II I II I I g041-l RGGGEFGPRWHQAAQGISKHKSVDDLLAVVRDLSERGMSSPKHIGLQGGSNGGLITAAAF 490 500 510 520 530 540 550 560 570 580 590 600 m0 1-l.pep VREPQSIGALVCEVPLTDMIRYPLLSAGSSWTDEYGNPQKYEVCKRRLGELSPYHNLSDG II I II I I II II II I I I I II II I I I II II II I II II II II I II : II I II II II II II I II I g041-l VREPQSIGALVCEVPLTDMIRYPLLSAGSSWTDEYGNPQKYEACKRRLGELSPYHNLSDG 550 560 570 580 590 600 610 620 630 640 650 660 m041-l.pep IDYPPALITTSLSDDRVHPAHALKFYAKLRETSAQSWLYSPDGGGHTGNGTQRESADELA I I II I II I II II I I II I II I II I II I II I II I I I I II II II II II II I I I I II I II : I I g041-l IDYPPALITTSLSDDRVHPAHALKFYAKLRETSPQSWLYSPDGGGHTGNGTQRESADKLA 610 620 630 640 650 660 670 m041-l.pep CVLLFLKEFLGX I II I I I I I I I I I g041-l CVLLFLKEFLGX 670 m041-l/P55577 sp|P55577| Y4NA_RHISN PROBABLE PEPTIDASE Y4NA >gi|2182536 (AE000086) Y4nA [Rhizobium sp. NGR234] Length = 726 Score = 370 bits (940), Expect = e-101 Identities = 217/682 (31%), Positives = 331/682 (47%), Gaps = 22/682 (3%) Query: 2 KSYPDPYRHFENLDSAETQNFAAEANAETRARFLENDKARALSDGILAQLQDTRQIPFCQ 61 K DP + +D + + N T + ++ + L LQ T +1 Sbjct: 42 KDASDPRAYLNEIDGDKAMTWVEAHNLSTVDKLΞKDPRYSEYQADALTILQATDRIASPS 101 Query: 62 EHRARMY-HFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSVADFDELLGDDVYLGGVΞH 120 R M +F QD + +G++R T +YRSG P+W+ + V + G G Sbjct: 102 FARDGMIDNFWQDGTHVQGLWRRTTWESYRSGNPQWRTILDVDALSKAEGKTWVFEGGDC 161 Query: 121 LVEQPNRALLTLSKLGSDTAYTLEVDLEAGELVEGGFHFPAGKNHVSKRDENSVWVCPAW 180 L N L+ LS G D E D+ GE V+ GF P GK V+W DEN+++V W Sbjct: 162 PPTSNLCLIRLSDGGKDADWREFDIAKGEFVKEGFVLPEGKQSVTWVDENTIYVTREW 221 Query: 181 NERQLTQSGYPREVWLVERGKSFEESLPVYQ IGEDGMM—VNAWRYLDPQGSPI 232 ++T SGY +V+RG+S ++++ +++ E G++ ++ +D + Sbjct: 222 TPGEVTSSGYAYVTKVVKRGQΞLDQAVEIFRGQKKDVSAERGVLRDIDGKYVMDTSYRGL 281 Query: 233 DLIEASDGFYTKTYLRVSAEGEAKPLNLPNDCDVVGYLAGHLLLTLRKDWNRANQS-YPS 291 D FY + + L LP GY G + L+ DW A + + + Sbjct: 282 DFFNTELAFYPNGH PDTRK LPLPTTAVFSGYYKGQAIYWLKSDWTSAKGTVFHN 337 Query: 292 GALVAVKLNRGELGAAQL LFAPDETQALEΞVETTKRFWASLLENVQGRLKAWRFA 347 GA++A L A++ LF P+E Q++ TK +V S+L NV ++++ F Sbjct: 338 GAIIAFDLKAALADPARVEPLVLFMPNEHQSVAGTTQTKNRLVLSILSNVTSEVRSFDFG 397 Query: 348 DGKWQEVELPRLPSGALEMTDQPWGGDVVYLAASDFTTPLTLFALDLNVMELTVMRRQPQ 407 G W +L + L +T D +++ + F P TLF D ++ + P Sbjct: 398 KGGWSSFKLALPENSTLSLTSSDDESDQLFVFSEGFLEPSTLFCADAATGQVEKITSTPA 457 Query: 408 QFDSDGINVQQFWTTSADGERIPYFHVGKNAAP DMPTLVYAYGGFGIPELPHYLGSI 464 +FD+ G+ QQFW TS DG ++PYF V + PT++YAYGGF IP P Y + Sbjct: 458 RFDAGGLQAQQFWATSKDGTKVPYFLVARKDVKLDGTNPTILYAYGGFQIPMQPSYSAVL 517 Query: 465 GKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLAVVRDLSERGISSPEHI 524 GK WLE+G A+ LANIRGGGEFGP+WH A ++ + DD AV +DL + ++S H+ Sbjct: 518 GKLWLEKGGAYALANIRGGGEFGPKWHDAGLKTNRQRVYDDFQAVAQDLIAKKVTSTPHL 577 Query: 525 GLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLSAGSSWTDEYGNPQKYEVC 584 G+ GGSNGGL+ ++ P A+V +VPL DM+ + +SAG+SW EYG+P V Sbjct: 578 GIMGGSNGGLLMGVQMIQRPDLWNAVVIQVPLLDMVNFTRMSAGASWQAEYGSPDD-PVE 636 Query: 585 KRRLGELSPYHNLSDGIDYPPALITTSLΞDDRVHPAHALKFYAKLRETSAQSWLYSPDGG 644 L +SPYHN+ G+ YP TS DDRV P HA K A + + Y G Sbjct: 637 GAFLRSISPYHNVKAGVAYPEPFFETSTKDDRVGPVHARKMAALFEDMGLPFYYYENIEG 696 Query: 645 GHTGNGTQRESADELACVLLFL 666 GH +E A A +++ Sbjct: 697 GHAAAANLQEHARRYALEYIYM 718 The following partial DNA sequence was identified in N meningitidis <SEQ ID 163>: a041-l .seq 1 ATGAAATCCT ACCCCGACCC CTACCGCCAT TTTGAAAACC TCGATTCCGC 51 CGAAACGCAA AACTTCGCTG CTGAAGCGAA TGCCGAAACG CGCGCGCGTT 101 TTTTAAACAA CGACAAGGCA CGCGCATTGT CTGACGGCAT TTTGGCGCAG 151 TTGCAGGACA CGCGGCAAAT TCCGTTTTGT CAGGAACACC GCGCGCGGAT 201 GTACCATTTC CATCAAGATG CGGAATATCC GAAAGGCGTG TACCGCGTGT 251 GTACCGCGGC GACTTACCGT TCGGGCTATC CTGAGTGGAA AATCCTGTTT 301 TCGGTGGCGG ATTTCGACGA ATTGCTCGGT GACGATGTAT ATCTAGGCGG 351 CGTGTCGCAC CTGGTGGAAC AGCCCAACCG CGCGTTGTTA ACACTGAGCA 401 AATCGGGCGG CGATACCGCG TACACGCTGG AAGTGGATTT GGAAGCAGGG 451 GAGTTGGTAG AAGGCGGTTT TCACTTTCCG GCAGGCAAAA ACCATGTGTC 501 GTGGCGCGAT GAAAACAGCG TGTGGGTGTG TCCGGCTTGG GACGAACGCC 551 AGTTGACCGA ATCGGGCTAT CCGCGCGAGG TGTGGCTGGT GGAACGCGGC 601 AAGAGTTTCG AGGAAAGCCT GCCGGTGTAC CAAATTGCTG AAGACGGCAT 651 GATGGTGAAC GCGTGGCGTT ACCTCGATCC GCAGGGTTCG CCGATTGATT 701 TGATTGAAGC GTCTGACGGT TTTTACACCA AAACCTATTT GCAGGTCTCA 751 GCCGAAGGCG AAGCGAAACC GTTAAACCTG CCCAACGATT GCGACGTAGT 801 CGGCTATCTG GCCGGACATC TTTTGCTGAC CTTGCGTAAA GACTGGCACC 851 GCGCGAACCA AAGCTATCCG AGTGGCGCAT TGGTAGCAGT AAAATTAAAC 901 CGCGGCGAAT TGGGCGCGGC GCAGCTTTTG TTTGCGCCCA ATGAAACGCA 951 GGCATTGGAA AGCGTGGAAA CGACCAAGCG TTTTGTCGTG GCGAGCCTGC 1001 TGGAAAACGT ACAGGGTCGT CTGAAAGCGT GGCGTTTTAC TGATGGCAAA 1051 TGGCAGGAAA CCGAGTTGCC GCGCCTGCCT TCGGGCGCGT TGGAAATGAC 1101 CGACCAACCG TGGGGGGGCG ACGTAGTTTA CCTTGCCGCC AGCGATTTCA 1151 CCACGCCGCT GACGCTGTTT GCATTGGATT TGAACGTGAT GGAACTGACC 1201 GTCATGCGCC GCCAGCCGCA GCAGTTTGAT TCAGACGGCA TTAACGTGCA 1251 GCAGTTTTGG ACGACTTCGG CTGACGGCGA GCGCATTCCT TATTTCCACG 1301 TCGGCAAAAA CGCCGCGCCC GACATGCCGA CGCTGGTCTA TGCCTACGGC 1351 GGTTTCGGCA TTCCCGAATT GCCGCATTAT CTGGGCAGCA TTGGCAAATA 1401 TTGGCTGGAA GAGGGCAATG CCTTTGTATT GGCGAACATC CGCGGCGGCG 1451 GCGAGTTCGG CCCGCGCTGG CATCAGGCGG CGCAGGGAAT CAGCAAACAT 1501 AAAAGCGTTG ATGATTTATT GGCAGTCGTG AGCGATTTGT CCGAACGCGG 1551 TATCAGTTCG CCCGAACACA TCGGCTTGCA GGGCGGCAGC AACGGCGGAC 1601 TGATTACTGC CGCCGCCTTC GTGCGCGAAC CGCAAAGCAT AGGCGCGCTG 1651 GTGTGCGAAG TGCCGCTGAC CGACATGATC CGTTATCCGC TGCTCTCCGC 1701 CGGTTCAAGC TGGACAGACG AATACGGCAA TCCGCAAAAA TACGAAGTCT 1751 GCAAACGCCG GTTGGGCGAA TTGTCGCCGT ATCACAATCT TTCAGACGGC 1801 ATCGATTATC CGCCCGCGCT CATTACCACC AGCCTGTCCG ACGATCGCGT 1851 CCATCCCGCC CACGCGCTCA AGTTCTACGC CAAACTGCGC GAAACCTCGC 1901 CGCAATCTTG GCTCTACTCG CCTGACGGCG GCGGCCATAC CGGCAACGGC 1951 ACGCAGCGCG AAGCCGCCGA CGAACTCGCC TGCGTGTTGC TGTTTTTGAA 2001 AGAGTTTTTG GGCTAA This corresponds to the amino acid sequence <SEQ ID 164; ORF 041-l.a>: a041-l .pep 1 MKSYPDPYRH FENLDSAETQ NFAAEANAET RARFLNNDKA RALSDGILAQ 51 LQDTRQIPFC QEHRARMYHF HQDAEYPKGV YRVCTAATYR SGYPEWKILF 101 SVADFDELLG DDVYLGGVSH LVEQPNRALL TLSKSGGDTA YTLEVDLEAG 151 ELVEGGFHFP AGKNHVSWRD ENSVWVCPAW DERQLTESGY PREVWLVERG 201 KSFEESLPVY QIAEDGMMVN AWRYLDPQGS PIDLIEASDG FYTKTYLQVS 251 AEGEAKPLNL PNDCDWGYL AGHLLLTLRK DWHRANQSYP SGALVAVKLN 301 RGELGAAQLL FAPNETQALE SVETTKRFW ASLLENVQGR LKAWRFTDGK 351 WQETELPRLP SGALEMTDQP WGGDWYLAA SDFTTPLTLF ALDLNVMELT 401 VMRRQPQQFD SDGINVQQFW TTSADGERIP YFHVGKNAAP DMPTLVYAYG 451 GFGIPELPHY LGSIGKYWLE EGNAFVLANI RGGGEFGPRW HQAAQGISKH 501 KSVDDLLAVV SDLSERGISΞ PEHIGLQGGS NGGLITAAAF VREPQSIGAL 551 VCEVPLTDMI RYPLLSAGSS WTDEYGNPQK YEVCKRRLGE LSPYHNLSDG 601 IDYPPALITT SLSDDRVHPA HALKFYAKLR ETSPQSWLYS PDGGGHTGNG 651 TQREAADELA CVLLFLKEFL G* a041-l/m041-l 97.9% identity in 671 aa overlap 10 20 30 40 50 60 aO41-1. pep MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLNNDKARALSDGILAQLQDTRQIPFC I II II II II II I I II II I II II I I I I I I I II II I I : I II II II I II II II II II II II II m041-l MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLENDKARALSDGILAQLQDTRQIPFC 10 20 30 40 50 60 70 80 90 100 110 120 a041-l.pep QEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSVADFDELLGDDVYLGGVSH I I I I II I I II II II I I II I II II I I I II II II I I I II I II II II II I I II I II II II I II m041-l QEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSVADFDELLGDDVYLGGVSH 70 80 90 100 110 120 130 140 150 160 170 180 a041-l . pep LVEQPNRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFPAGKNHVSWRDENSVWVCPAW I II I II I I II I I II I: I I II II I II II I I II I II II I II I II I II I II II II II I II II m041-l LVEQPNRALLTLSKLGSDTAYTLEVDLEAGELVEGGFHFPAGKNHVSWRDENSVWVCPAW 130 140 150 160 170 180 190 200 210 220 230 240 a041-l.pep DERQLTESGYPREVWLVERGKSFEESLPVYQIAEDGMMVNAWRYLDPQGSPIDLIEASDG : II II I : II I I I II II II I II II I I I I I I II I : II II I II II II II I II II II II I II I I m041-l NERQLTQSGYPREVWLVERGKSFEESLPVYQIGEDGMMVNAWRYLDPQGSPIDLIEASDG 190 200 210 220 230 240 250 260 270 280 290 300 a041-l . pep FYTKTYLQVSAEGEAKPLNLPNDCDWGYLAGHLLLTLRKDWHRANQSYPSGALVAVKLN II II I I I : I II II I II II I II II I II II I I II II I II I I I II : I II I II I I II I II II II m041-l FYTKTYLRVSAEGEAKPLNLPNDCDWGYLAGHLLLTLRKDWNRANQSYPSGALVAVKLN 250 260 270 280 290 300 310 320 330 340 350 360 a041-l.pep RGELGAA Q LLFAPNET Q ALESVETTKRFVVASLLENVQGRLKAWRFTDGKWQETELPRLP I I M I I I M II M: II II I II II I I I II I M I I M II II II II I II: II I II I: I M M I m0 1-l RGELGAA Q LLFAPDET Q ALESVETTKRFVVASLLENVQGRLKAWRFADGKWQEVELPRLP 310 320 330 340 350 360 370 380 390 400 410 420 a041-l.pep SGALEMTDQPWGGDWYLAASDFTTPLTLFALDLNVMELTVMRRQPQQFDSDGINVQQFW I I II II II II I II I I I II II II I II II I II I II I II II II I II I I II I I II I II II II II m041-l SGALEMTDQPWGGDWYLAASDFTTPLTLFALDLNVMELTVMRRQPQQFDSDGINVQQFW 370 380 390 400 410 420 430 440 450 460 470 480 a041-l.pep TTSADGERIPYFHVGKNAAPDMPTLVYAYGGFGIPELPHYLGSIGKYWLEEGNAFVLANI I I II II I I II II II II II II II II I II I II I I II II I I II II I II II I II I II I II I M I m041-l TTSADGERIPYFHVGKNAAPDMPTLVYAYGGFGIPELPHYLGSIGKYWLEEGNAFVLANI 430 440 450 460 470 480 490 500 510 520 530 540 a041-l.pep RGGGEFGPRWHQAAQGISKHKSVDDLLAWSDLSERGISSPEHIGLQGGSNGGLITAAAF I II II II II II II I II II II I II II II II I I II II I II II II I II II II II II II II II m041-l RGGGEFGPRWHQAAQGISKHKSVDDLLAVVRDLSERGISSPEHIGLQGGSNGGLITAAAF 490 500 510 520 530 540 550 560 570 580 590 600 a041-l.pep VREPQSIGALVCEVPLTDMIRYPLLSAGSSWTDEYGNPQKYEVCKRRLGELSPYHNLSDG I II II II II II I I II II II II II I II II I I II I II I II II I II II II I I II II II II II I m041-l VREPQSIGALVCEVPLTDMIRYPLLSAGSSWTDEYGNPQKYEVCKRRLGELSPYHNLSDG 550 560 570 580 590 600 610 620 630 640 650 660 a041-l.pep IDYPPALITTSLSDDRVHPAHALKFYAKLRETSPQSWLYSPDGGGHTGNGTQREAADELA I II II II II II I I II I I II I I II I II II I II II II II II II II II I II I II I I : II I I I m041-l IDYPPALITTSLSDDRVHPAHALKFYAKLRETSAQSWLYSPDGGGHTGNGTQRESADELA 610 620 630 640 650 660 670 a041-l.pep CVLLFLKEFLGX II I II II II II I m041-l CVLLFLKEFLGX 670 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 165>: g042. seq 1 ATGACGATGA TTTGCTTGCG CTTCCAagcG TTCGTGCCGC ATACCAGCGC 51 GTTATCCAAC ACTTCCACGG CAGCCGGCCC TTCCTGCCCG ATGGCGGCGG 101 TGCGGTCGAT GATGAAAATC CAGCCGGGGT TTTTCTCTTT GATGTATTCG 151 AAGGAAACGG GCTGCCCGTG CCCTTCGTTG CGTAAAGATT CGTCCACGGG 201 CGGCAGGCCG ATGTCGCCGT GTATCCAACT TGCCAACCGC GATTGCGTGC 251 CGAAGGCGGA CACCTTGTTG CCTGTAACCG ACAGCACCAG CCCGCGTCCT 301 TTGCCTTTGG cggCTTCGCG CTTTTGGGCG AACAGCGCGT CAATCTGCGC 351 ATTCAATTCC GCCACGCGCG CTTCCTTACC GAAAATCCGC GACAGGGTCT 401 CCATCTGCTT CTCGCCGCTG GTGCGGATAT TGCCGTTGTC CACCGTCAAA 451 TCTATGgtgG TCGCGTTTTT CGCCAACTGT TCATACGCTT CCGCACCCGG 501 CCCGCCGGTA ATGACAAACT GCGGATTGTG GCGGTGCAGG GATTCGCAAT 551 CGGGCTCAAA CAGCGTCCCC ACCGTTGCCG CCTTGTCAAA TGCAGGCTGC 601 AAATAG This corresponds to the amino acid sequence <SEQ ID 166; ORF 042.ng>: g042.pep 1 MTMICLRFQA FVPHTSALSΝ TSTAAGPSCP MAAVRSMMKI QPGFFSLMYS 51 KETGCPCPSL RKDSSTGGRP MSPCIQLAΝR DCVPKADTLL PVTDSTSPRP 101 LPLAASRFWA ΝSASICAFΝS ATRASLPKIR DRVSICFSPL VRILPLSTVK 151 SMVVAFFAΝC SYASAPGPPV MTΝCGLWRCR DSQSGSΝSVP TVAALSΝAGC 201 K* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 167>: m042. seq 1 ATGACGATGA TTTGCTTGCG CTTCCAAGCG TTCGTGCCGC GTACCAGCGC 51 GTTATCCAmT ACTTCGACAG CCGcCGGCCy TTCyTGCCCG ATGGCGGCGG 101 TACGGTCGAT GATGAAAATC CAATCGGGGT TTTTCTCTTT GATGTATTCG 151 AAGGAAACAG GCTGCCCGTG CCCCTCGTTG CGTAAAGATT CGTCTACAGG 201 CGGTAGGCCG ATGTCGCCGT GTATCCAACT TGCCAACCGC GACTGCGTGC 251 CGAAGGCGGA CACCTTGTTG CCCGTAACCG ACAGCACCAG CCCGCGTCCT 301 TTGCCTTTGG CGGCTTCGCG CGTTTGGGCG AACAGCGCGT CAATCTGCGC 351 CTTCAATTCC GCCGCGCGCG CTTCCTTGCC GAAAATCCGC GCCAAGGTCT 401 CCATCTGCTT TTCGCCGCTG GTGCGGATAT TGCCGTTGTC CACCGTCAGA 451 TCTATGGTGG TCGCGTTTTT CGCTAACTGT TCATACGCTT CCGCGCCCGG 501 CCCGCCGGTA ATGACAAGCT GAGGATTGTA GCGGTGCAGG GCTTCGTAAT 551 CGGGCTCGAA CAGCGTCCCC ACCGTTGCCG CCTTGTCAAA TGCAGGCTGC 601 AAATAA This corresponds to the amino acid sequence <SEQ ID 168; ORF 042>: m042.pep 1 MTMICLRFQA FVPRTSALSX TSTAAGXSCP MAAVRSMMKI QSGFFSLMYS 51 KETGCPCPSL RKDSSTGGRP MSPCIQLANR DCVPKADTLL PVTDSTSPRP 101 LPLAASRVWA NSASICAFNS AARASLPKIR AKVSICFSPL VRILPLSTVR 151 SMWAFFANC SYASAPGPPV MTSXGLXRCR ASXSGSNSVP TVAALSNAGC 201 K* The following partial DNA sequence was identified in N meningitidis <SEQ ID 169>: a042.seq 1 ATGACGATGA TTTGCTTGCG CTTCCAAGCG TTCGTGCCGC GTACCAGCGC 51 GTTATCCAAT ACTTCGACAG CCGCCGGCCC TTCCTGCCCG ATGGCGGCGG 101 TACGGTCGAT GATGAAAATC CAATCGGGGT TTTTCTCTTT GATGTATTCG 151 AAGGAAACAG GCTGCCCGTG CCCCTCGTTG CGTAAAGATT CGTCTACAGG 201 CGGTAGGCCG ATGTCGCCGT GTATCCAACT TGCCAACCGC GACTGCGTGC 251 CGAAGGCGGA CACCTTGTTG CCCGTAACCG ACAGCACCAG CCCGCGTCCT 301 TTGCCTTTGG CGGCTTCGCG CGTTTGGGCG AACAGCGCGT CAATCTGCGC 351 CTTCAATTCC GCCGCGCGCG CTTCCTTGCC GAAAATCCGC GCCAAGGTCT 401 CCATCTGCTT TTCGCCGCTG GTGCGGATAT TGCCGTTGTC CACCGTCAGA 451 TCTATGGTGG TCGCGTTTTT CGCCAACTGT TCATACGCTT CCGCGCCCGG 501 CCCGCCGGTA ATGACAAGCT GAGGATTGTA GCGGTGCAGG GCTTCGTAAT 551 CGGGCTCGAA CAGCGTCCCC ACCGTTGCCG CCTTGTCAAA TGCAGGCTGC 601 AAATAA This corresponds to the amino acid sequence <SEQ ID 170; ORF 042. a>: a042.pep 1 MTMICLRFQA FVPRTSALSΝ TSTAAGPSCP MAAVRSMMKI QSGFFSLMYS 51 KETGCPCPSL RKDSSTGGRP MSPCIQLAΝR DCVPKADTLL PVTDSTSPRP 101 LPLAASRVWA ΝSASICAFΝS AARASLPKIR AKVSICFSPL VRILPLSTVR 151 SMVVAFFAΝC SYASAPGPPV MTS*GL*RCR AS*SGSΝSVP TVAALSNAGC 201 K* m042/a042 99.0%o identity over a 201 aa overlap 10 20 30 40 50 60 m042.pep MTMICLRFQAFVPRTSALSXTSTAAGXSCPMAAVRSMMKIQSGFFSLMYSKETGCPCPSL II II I II II II I I II II II llllll I I II II I I II I II II I II II I I I II I II I I II I a042 MTMICLRFQAFVPRTSALSNTSTAAGPSCPMAAVRSMMKIQSGFFSLMYSKETGCPCPSL 10 20 30 40 50 60 70 80 90 100 110 120 m042.pep RKDSSTGGRPMSPCIQLANRDCVPKADTLLPVTDSTSPRPLPLAASRVWANSASICAFNS I II I II II II I II II I I I II II I I II I I I I I I I I II I I I II I II II II I II I I II I II I I a042 RKDSSTGGRPMSPCIQLANRDCVPKADTLLPVTDSTSPRPLPLAASRVWANSASICAFNS 70 80 90 100 110 120 130 140 150 160 170 180 m042.pep AARASLPKIRAKVSICFSPLVRILPLSTVRSMWAFFANCSYASAPGPPVMTSXGLXRCR II I I II I I II I II I I II II I I II I II I II I I I I I II I II II II II II I I II I I II II II I a042 AARASLPKIRAKVSICFSPLVRILPLSTVRSMWAFFANCSYASAPGPPVMTSXGLXRCR 130 140 150 160 170 180 190 200 m042.pep ASXSGSNSVPTVAALSNAGCKX I II II II I II II II II I I II I I a042 ASXSGSNSVPTVAALSNAGCKX 190 200 Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 042 shows 93.0% identity over a 201 aa overlap with a predicted ORF (ORF 042.ng) from N gonorrhoeae: m042/g042 10 20 30 40 50 60 m042.pep MTMICLRFQAFVPRTSALSXTSTAAGXSCPMAAVRSMMKIQSGFFSLMYSKETGCPCPSL I II II I II II II I : II II I llllll I I I II II I I II II I I II II II II I I II II II I g042 MTMICLRFQAFVPHTSALSΝTSTAAGPSCPMAAVRSMMKIQPGFFSLMYSKETGCPCPSL 10 20 30 40 50 60 70 80 90 100 110 120 m042.pep RKDSSTGGRPMSPCIQLAΝRDCVPKADTLLPVTDSTSPRPLPLAASRVWAΝSASICAFΝS I II II II I II II II II II II II II I II I I I II II I I II II I II II II II I I II I II I II g042 RKDSSTGGRPMSPCIQLAΝRDCVPKADTLLPVTDSTSPRPLPLAASRFWAΝSASICAFΝS 70 80 90 100 110 120 130 140 150 160 170 180 m042.pep AARASLPKIPAKVSICFSPLVRILPLSTVRSMWAFFAΝCSYASAPGPPVMTSXGLXRCR I : I I I II I I I : II II II I I II II I II I I : II I II I I II II II II I I I II I I : II III g042 ATRASLPKIRDRVSICFSPLVRILPLSTVKSMWAFFAΝCSYASAPGPPVMTΝCGLWRCR 130 140 150 160 170 180 190 200 m042.pep ASXSGSNSVPTVAALSNAGCKX I I I II II I II II II II II I I g042 DSQSGSNSVPTVAALSNAGCKX 190 200 The following partial DNA sequence was identified in N meningitidis <SEQ ID 171>: m042-l . seq 1 ATGACGATGA TTTGCTTGCG CTTCCAAGCG TTCGTGCCGC GTACCAGCGC 51 GTTATCCAAT ACTTCGACAG CCGCCGGCCC TTCCTGCCCG ATGGCGGCGG 101 TACGGTCGAT GATGAAAATC CAATCGGGGT TTTTCTCTTT GATGTATTCG 151 AAGGAAACAG GCTGCCCGTG CCCCTCGTTG CGTAAAGATT CGTCTACAGG 201 CGGTAGGCCG ATGTCGCCGT GTATCCAACT TGCCAACCGC GACTGCGTGC 251 CGAAGGCGGA CACCTTGTTG CCCGTAACCG ACAGCACCAG CCCGCGTCCT 301 TTGCCTTTGG CGGCTTCGCG CGTTTGGGCG AACAGCGCGT CAATCTGCGC 351 CTTCAATTCC GCCGCGCGCG CTTCCTTGCC GAAAATCCGC GCCAAGGTCT 401 CCATCTGCTT TTCGCCGCTG GTGCGGATAT TGCCGTTGTC CACCGTCAGA 451 TCTATGGTGG TCGCGTTTTT CGCTAACTGT TCATACGCTT CCGCGCCCGG 501 CCCGCCGGTA A This corresponds to the amino acid sequence <SEQ ID 172; ORF 042-l>: m042-l .pep 1 MTMICLRFQA FVPRTSALSΝ TSTAAGPSCP MAAVRSMMKI QSGFFSLMYS 51 KETGCPCPSL RKDSSTGGRP MSPCIQLAΝR DCVPKADTLL PVTDSTSPRP 101 LPLAASRVWA ΝSASICAFΝS AARASLPKIR AKVSICFSPL VRILPLSTVR 151 SMVVAFFAΝC SYASAPGPPV MTS* m042-l/g042 95.4% identity in 173 aa overlap 10 20 30 40 50 60 m042-l.pep MTMICLRFQAFVPRTSALSNTSTAAGPSCPMAAVRSMMKIQSGFFSLMYSKETGCPCPSL II II I I II II II I: II I II II I I I II II II I I II II I II II II II II II II I I I I I I I I g0 2 MTMICLRFQAFVPHTSALSNTSTAAGPSCPMAAVRSMMKIQPGFFSLMYSKETGCPCPSL 10 20 30 40 50 60 70 80 90 100 110 120 m042-l . pep RKDSSTGGRPMSPCIQLANRDCVPKADTLLPVTDSTSPRPLPLAASRVWANSASICAFNS I I II II II I I I II II I II I II I II I II II II II II I I II I II II II I I I II II II II I I g042 RKDSSTGGRPMSPCIQLANRDCVPKADTLLPVTDSTSPRPLPLAASRFWANSASICAFNS 70 80 90 100 110 120 130 140 150 160 170 m042-l .pep AARASLPKIRAKVSICFSPLVRILPLSTVRSMWAFFANCSYASAPGPPVMTSX I : I I II I II I : I II II II I II I I II I II : II I II II I II II II II I I I I II : g042 ATRASLPKIRDRVSICFSPLVRILPLSTVKSMWAFFANCSYASAPGPPVMTNCGLWRCR 130 140 150 160 170 180 g042 DSQSGSNSVPTVAALSNAGCKX 190 200 The following partial DNA sequence was identified in N meningitidis <SEQ ID 173>: a042-l.seq 1 ATGACGATGA TTTGCTTGCG CTTCCAAGCG TTCGTGCCGC GTACCAGCGC 51 GTTATCCAAT ACTTCGACAG CCGCCGGCCC TTCCTGCCCG ATGGCGGCGG 101 TACGGTCGAT GATGAAAATC CAATCGGGGT TTTTCTCTTT GATGTATTCG 151 AAGGAAACAG GCTGCCCGTG CCCCTCGTTG CGTAAAGATT CGTCTACAGG 201 CGGTAGGCCG ATGTCGCCGT GTATCCAACT TGCCAACCGC GACTGCGTGC 251 CGAAGGCGGA CACCTTGTTG CCCGTAACCG ACAGCACCAG CCCGCGTCCT 301 TTGCCTTTGG CGGCTTCGCG CGTTTGGGCG AACAGCGCGT CAATCTGCGC 351 CTTCAATTCC GCCGCGCGCG CTTCCTTGCC GAAAATCCGC GCCAAGGTCT 401 CCATCTGCTT TTCGCCGCTG GTGCGGATAT TGCCGTTGTC CACCGTCAGA 451 TCTATGGTGG TCGCGTTTTT CGCCAACTGT TCATACGCTT CCGCGCCCGG 501 CCCGCCGGTA A This corresponds to the amino acid sequence <SEQ ID 174; ORF 042-1. a>: a042-l.pep 1 MTMICLRFQA FVPRTSALSΝ TSTAAGPSCP MAAVRSMMKI QSGFFSLMYS 51 KETGCPCPSL RKDSSTGGRP MSPCIQLAΝR DCVPKADTLL PVTDSTSPRP 101 LPLAASRVWA ΝSASICAFΝS AARASLPKIR AKVSICFSPL VRILPLSTVR 151 SMVVAFFAΝC SYASAPGPPV MTS* m042-l/a042-l 100.0% identity in 173 aa overlap 10 20 30 40 50 60 m042-l.pep MTMICLRFQAFVPRTSALSΝTSTAAGPSCPMAAVRSMMKIQSGFFSLMYSKETGCPCPSL II I II I II I II I I I I I I II I I II I I I I II II I II II II II I II I I II I I I II II I II II I a042-l MTMICLRFQAFVPRTSALSΝTSTAAGPSCPMAAVRSMMKIQSGFFSLMYSKETGCPCPSL 10 20 30 40 50 60 70 80 90 100 110 120 m042-l.pep RKDSSTGGRPMSPCIQLAΝRDCVPKADTLLPVTDSTSPRPLPLAASRVWAΝSASICAFΝS II II II II I II II II I I I II I II I I I I II II I II II II II I II I II I I I II I II I II I II a042-l RKDSSTGGRPMSPCIQLAΝRDCVPKADTLLPVTDSTSPRPLPLAASRVWAΝSASICAFΝS 70 80 90 100 110 120 130 140 150 160 170 m042-l .pep AARASLPKIRAKVSICFSPLVRILPLSTVRSMWAFFAΝCSYASAPGPPVMTSX II II I II I II II I I I I II II II I II II II II I I I II II I I I I II II II II I II I a042-l AARASLPKIRAKVSICFSPLVRILPLSTVRSMVVAFFAΝCSYASAPGPPVMTSX 130 140 150 160 170 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 175>: g043. seq 1 ATGGTTGTTT CAAATCAAAA TATCTATGCC GTCGGCCCAT CAGCACTTTT 51 TCACATCCGA AGGCAAAAAT CCGTAATGCC GCCTGAACGC TTCgttgaAC 101 CGTCCCGCGT ggcggtagcc gcAAAAGTGC ATcGCGGCTT GGATGGTGCT 151 GCCCGATTCG ATGAGGGcga gcGCGTGTTC CAGCCGCAGG CGGCGCAGGC 201 GTCCGGCGAC GGTTTCGCCG GTTTGCGCTT TGAAATAGCG TTTCAGGTAG 251 CATTCGTTCA GCCCGACGCG GCGGGCGATT TCGGCGATGG TCAGCGGGCG 301 GGCGAATTCG CTGTTCAAAA TATCGGCGGC TTCGTCTATG CGCCGGCGGC 351 GGTAGCCGTT GTCGTGGCGG CGGAAGGTGA AGCGTAA This corresponds to the amino acid sequence <SEQ ID 176; ORF 043.ng>: g043.pep 1 MWSNQNIYA VGPSALFHIR RQKSVMPPER FVEPSRVAVA AKVHRGLDGA 51 ARFDEGERVF QPQAAQASGD GFAGLRFEIA FQVAFVQPDA AGDFGDGQRA 101 GEFAVQNIGG FVYAPAAVAV WAAEGEA* The following partial DNA sequence was identified in N meningitidis <SEQ ID 177>: m043. seq 1 ATGGTTGTTT CAAATCAAAA TATCTATGCC GCCGGCCCCT CAGCACTTCT 51 TCACATCCGA AGGCAAAAAT CCGTAATGCC GTCTGAACGC TTCGTTGAAC 101 CGTCCCGCGT GGCGGTAGCC GCAAAAGTGC ATGGCGGCTT GGACGGTGCT 151 GCCGGATTCG ATGAGGGCGA GCGCGTGTTC CAGCCGCAGG CGGCGCAgGC 201 ATCCGGCGAC GGTTTCGCCG GTTTGCGCTT TGAAATAGCG TTTCAGGTAG 251 CATTCGTTCA GTCCGACGCG GCGGGCGATT TCGGCGATGG TCAGCGGACG 301 GGCGAATTCG TGTTGCAGGA TGTCGGCGGC TTCGTCTATG CGCCGACGGC 351 GGTAACCGTT GTCGTGGCGG CGGAAGGTGA AGCGCAATAA This corresponds to the amino acid sequence <SEQ ID 178; ORF 043>: m043.pep 1 MWSΝQΝIYA AGPSALLHIR RQKSVMPSER FVEPSRVAVA AKVHGGLDGA 51 AGFDEGERVF QPQAAQASGD GFAGLRFEIA FQVAFVQSDA AGDFGDGQRT 101 GEFVLQDVGG FVYAPTAVTV WAAEGEAQ* Computer analysis of the amino acid sequences gave the following results: Homology with a predicted ORF from N meningitidis menA with menB ORF 043 shows 89.8% identity over a 128 aa overlap with a predicted ORF (ORF043.a) from N gonorrhoeae: m043/g043 10 20 30 40 50 60 mO 43 . pep MWSΝQΝI YAAGPSALLHIRRQKSVMPSERFVEPSRVAVAAKVHGGLDGAAGFDEGERVF I I I I I I I I I I : I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I l l l l l l I I I I I I I I g043 MWSΝQΝIYAVGPSALFHIRRQKSVMPPERFVEPSRVAVAAKVHRGLDGAARFDEGERVF 10 20 30 40 50 60 70 80 90 100 110 120 m043.pep QPQAAQASGDGFAGLRFEIAFQVAFVQSDAAGDFGDGQRTGEFVLQDVGGFVYAPTAVTV II II II I II I II II II II II I II I I I I I II I II I II I I : II I : : I : : II I I II I : I I : I g0 3 QPQAAQASGDGFAGLRFEIAFQVAFVQPDAAGDFGDGQRAGEFAVQΝIGGFVYAPAAVAV 70 80 90 100 110 120 130 mO43. pep WAAEGEAQX II II II I I g043 WAAEGEAXX 130 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 179>: a043. seq 1 ATGGTTGTTT CAAATCAAAA TATCTATGCC GCCGGCCCCT CAGCACTTCT 51 TCACATCCGA AGGCAAAAAT CCGTAATGCC GTCTGAACGC TTCGTTGAAC 101 CGTCCCGCGT GGCGGTAGCC GCAAAAGTGC ATGGCGGCTT GGACGGTGCT 151 GCCGGATTCG ATGAGGGCGA GCGCGTGTTC CAGCCGCAGG CGGCGCAGGC 201 ATCCGGCGAC GGTTTCGCCG GTTTGCGCTT TGAAATAGCG TTTCAGGTAG 251 CATTCGTTCA GTCCGACGCG GCGGGCGATT TCGGCGATGG TCAGCGGACG 301 GGCGAATTCG TGTTGCAGGA TGTCGGCGGC TTCGTCTATG CGCCGACGGC 351 GGTAACCGTT GTCGTGGCGG CGGAAGGTGA AGCGCAATAA This corresponds to the amino acid sequence <SEQ ID 180; ORF 043. a>: a043.pep 1 MWSNQNIYA AGPSALLHIR RQKSVMPSER FVEPSRVAVA AKVHGGLDGA 51 AGFDEGERVF QPQAAQASGD GFAGLRFEIA FQVAFVQSDA AGDFGDGQRT 101 GEFVLQDVGG FVYAPTAVTV WAAEGEAQ* m043/a043 100.0% identity in 129 aa overlap 10 20 30 40 50 60 mO43. pep MWSNQNIYAAGPSALLHIRRQKSVMPSERFVEPSRVAVAAKVHGGLDGAAGFDEGERVF II I II I II I II II I I I II I I I II II II II II II I II II II I I II II I I II I II II II I II a043 MVVSNQNIYAAGPSALLHIRRQKSVMPSERFVEPSRVAVAAKVHGGLDGAAGFDEGERVF 10 20 30 40 50 60 70 80 90 100 110 120 m043.pep QPQAAQASGDGFAGLRFEIAFQVAFVQSDAAGDFGDGQRTGEFVLQDVGGFVYAPTAVTV I II II I II II I II I II II I I I I II I II II II II II I I I I II II I II II II I II I II II I I a043 QPQAAQASGDGFAGLRFEIAFQVAFVQSDAAGDFGDGQRTGEFVLQDVGGFVYAPTAVTV 70 80 90 100 110 120 130 O43. pep WAAEGEAQX II I II I II II a043 WAAEGEAQX 130 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 181>: g044.seq 1 ATGCTGCCCG ACCAGAGCGT CGAGTTCTTG CCACAAGTCG TCGTTTTTGA 51 CGGGCTGTTT GGCGGCGGTT TTCCAGCCGT TGCGCTTCCA ACCGTGTATC 101 CAGTTTTCCA TGCCGTTTTT GACGTATTGC GAGTCGGTGC AGATGATGAC 151 GGTGCAGCGG CGTTTGAGCG ATTTCAGCCC TTCGATAACG GCGGTCAGCT 201 CCATGCGGTT GTTGGTGGTT TGCGCTTCGC CGCCGAAAAG TTCTTTTTCG 251 CGGCTGCCGT AGCGCATTAA This corresponds to the amino acid sequence <SEQ ID 182; ORF 044.ng>: g044.pep 1 MLPDQSVEFL PQVWFDGLF GGGFPAVALP TVYPVFHAVF DVLRVGADDD 51 GAAAFERFQP FDΝGGQLHAV VGGLRFAAEK FFFAAAVAH* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 183>: m044. seq 1 ATGCCGTCCG ACTAGAGCGT CGAGTTCTTT CCAGAAGTCG TCGTTTTTGA 51 CGGGCTGTTT GGAGGCGGTT TTCCAGCCGT TGCGCTTCCA ACCGTGTATC 101 CAGTTTTCCA TGCCATTTTT GACGTATTGC GAGTCGGTGC AGATGATGAC 151 GGTGCAGCGG CGTTTGAGCG ATTTCAGTCC TTCGATGACG GCAGTCAGTT 201 CCATGCGGTT GTTGGTGGTT TGCGCTTCGC CGCCGAAAAG TTCTTTTTCG 251 TGGCTACCGT AGCGCAyTAa This corresponds to the amino acid sequence <SEQ ID 184; ORF 044>: m044.pep 1 MPSDXSVEFF PEWVFDGLF GGGFPAVALP TVYPVFHAIF DVLRVGADDD 51 GAAAFERFQS FDDGSQFHAV VGGLRFAAEK FFFVATVAH* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 185>: a044. seq 1 GTGCCGTCCG ACCAGCGCGT CGAGTTCTTT CCACAAGTCG TCGTTTTTGA 51 CGGGCTGTTT GGCGGCGGTT TTCCAGCCGT TGCGCTTCCA ACCGTGTATC 101 CAGTTTTCCA TGCCGTTTTT GACGTATTGC GAGTCGGTGC AGATGATGAC 151 GGTGCAGCGG CGTTTGAGCG ATTTCAGTCC TTCGATGACG GCGGTCAGTT 201 CCATACGGTT GTTGGTGGTT TGCGCTTCGC CGCCGAAAAG TTCTTTTTCG 251 TGGCTGCCGT AGCGCATTAA This corresponds to the amino acid sequence <SEQ ID 186; ORF 044.a>: a044.pep 1 VPSDQRVEFF PQVWFDGLF GGGFPAVALP TVYPVFHAVF DVLRVGADDD 51 GAAAFERFQS FDDGGQFHTV VGGLRFAAEK FFFVAAVAH* m044/a044 91.0%> identity over a 89 aa overlap 10 20 30 40 50 60 m044.pep MPSDXSVEFFPEVWFDGLFGGGFPAVALPTVYPVFHAIFDVLRVGADDDGAAAFERFQS M I I II I : I II I II II II I II I I II I II II I I II : I I II I II II I II II II II II I a044 VPSDQRVEFFPQWVFDGLFGGGFPAVALPTVYPVFHAVFDVLRVGADDDGAAAFERFQS 10 20 30 40 50 60 70 80 90 m044. pep FDDGSQFHAWGGLRFAAEKFFFVATVAHX II II : I II : I II I II I II I II I I II : I II I a044 FDDGGQFHTWGGLRFAAEKFFFVAAVAHX 70 80 90 Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 044 shows 86.5% identity over a 89 aa overlap with a predicted ORF (ORF 044.ng) from N gonorrhoeae: m044/g044 10 20 30 40 50 60 m044.pep MPSDXSVEFFPEVWFDGLFGGGFPAVALPTVYPVFHAIFDVLRVGADDDGAAAFERFQS I I g044 MLPDQSVEFLPQVWFDGLFGGGFPAVALPTVYPVFHAVFDVLRVGADDDGAAAFERFQP 10 20 30 40 50 60 70 80 90 O 44 . pep FDDGSQFHAWGGLRFAAEKFFFVATVAHX g044 FDΝGGQLHAWGGLRFAAEKFFFAAAVAHX 70 80 90 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 187>: g046. seq 1 ATGTCGGCAA TGCTGCGTCC GACAAGCAGC CCGCCGCgcc gCGCCTGTAT 51 GATGACCATC CGCACGCGGT CGTCTGCAAA ACGTAAAACC TGCAATGCGC 101 CCGGGCAGTC TATCAGGCCG GCAAGCTGTT CGGTAACGAG CTGTTCGGGG 151 CTGATGGTTT CGGTTATGCC gaATATGGAA AGGCTGCCGt TTTcGTTGTT 201 TTCGAGCTTG GGGCTGAGGT ATTCGAGGTA TtcgctGGAA CGGACGCGCG 251 CGATGCGGCC GGGGATGTTG AACAGGTCGG CGGCAACTTT GCAGGCGACG 301 ATGTTGGTTT CGTCGCTGCG GGagaGCGCG AGcagcaagt cggcatcttC 351 CgcgccggcG Cgttataatg tgAAGGGGGA TGCGccgttg ccgaAAACGG 401 TTTGGacatc gaggcggctg CCTGTTTCCT GCAATGCTTT TTCGTCGATG 451 TCGATAAcgg TTACGTCGTT GTTGGTGATG GCGGCAAGGT TTTGCGCGAC 501 GGTAGAACCT ACCTGCCCGT TGCCTAAAAT GAGGATTTTC ACGGTATGGG 551 TCGCCGGGTG A This corresponds to the amino acid sequence <SEQ ID 188; ORF 046.ng>: g046 -pep 1 MSAMLRPTSS PPRRACMMTI RTRSSAKRKT CNAPGQSIRP ASCSVTSCSG 51 LMVSVMPNME RLPFSLFSSL GLRYSRYSLE RTRAMRPGML NRSAATLQAT 101 MLVSSLRESA SSKSASSAPA RYNVKGDAPL PKTVWTSRRL PVSCNAFSSM 151 SITVTSLLVM AARFCATVEP TCPLPKMRIF TVWVAG* The following partial DNA sequence was identified in N meningitidis <SEQ ID 189>: m046. seq 1 ATGTCGGCAA TGCTGCGTCC GACAAGCAsT CCGC.r.sGC gCGcCTGTAT 51 GATGACCATC CGCACGCGGT CGTCTGCAAA ACGTAAAACC TGCAATGCGC 101 CCGGGCAGTC TATCAGGCCG GCAAGCTGTT CGGTAACGAG CTGTTCGGGG 151 CTGATGGTTT CGGTTATGCC GAATATGGAA AGGCTGCCGT TTTCGTTGTT 201 TTCGAGCTTG GGGCTGAGGT ATTCGAGGTA TTCGCTGGAA CGGACGCGCG 251 CGATGCGGCC GGGGATGTTG AACAGGTCGG CGGCAACTTT GCAGGCGACG 301 ATGTTGGTTT CGTCGCTGCG GGAGAGCGCG AGCAGCAAGT CGGCATCTTC 351 CGCGCCGGCG CGTTCTAATG TGAAGGGGGA TGCGCCGTTG CCGAAAACGG 401 TTTGGACATC GAGGCGGCTG CCTGTTTCCT GCAATGCTTT TTCGTCGATG 451 TCGATAACGG TTACGTCGTT GTTGGGTATG GCGGCAAGGT TTTGTGCGAC 501 GGTAGAACCT ACCTGTCCGT TGCCTAAAAT GAGGATTTTC ACGGTGTGGG 551 TCGCCGAGTG A This corresponds to the amino acid sequence <SEQ ID 190; ORF 046>: m046.pep 1 MSAMLRPTSX PXXRACMMTI RTRSSAKRKT CNAPGQSIRP ASCSVTSCSG 51 LMVSVMPNME RLPFSLFSSL GLRYSRYSLE RTRAMRPGML NRSAATLQAT 101 MLVSSLRESA SSKSASSAPA RSNVKGDAPL PKTVWTSRRL PVSCNAFSSM 151 SITVTSLLGM AARFCATVEP TCPLPKMRIF TVWVAE* The following partial DNA sequence was identified in N meningitidis <SEQ ID 191>: a046. seq 1 ATGTCGGCAA TGCTGCGTCC GACAAGCAGT CCGCCGCGCC GCGCCTGTAT 51 GATGACCATC CGCACGCGGT CGTCTGCAAA ACGTAAAACC TGCAATGCGC 101 CCGGGCAGTC TATCAGGCCG GCAAGCTGTT CGGTAACGAG CTGTTCGGGG 151 CTGATGGTTT CGGTTATGCC GAATATGGAA AGGCTGCCGT TTTCGTTGTT 201 TTCGAGCTTG GGGCTGAGGT ATTCGAGGTA TTCGCTGGAA CGGACGCGCG 251 CGATGCGGCC GGGGATGTTG AACAGGTCGG CGGCAACTTT GCAGGCGACG 301 ATGTTGGTTT CGTCGCTGCG GGAGAGCGCG AGCAGCAAGT CGGCATCTTC 351 CGCGCCGGCG CGTTCTAATG TGAAGGGGGA TGCGCCGTTG CCGAAAACGG 401 TTTGGACATC GAGGCGGCTG CCTGTTTCCT GCAATGCTTT TTCGTCGATG 451 TCGATAACGG TTACGTCGTT GTTGGGTATG GCGGCAAGGT TTTGTGCGAC 501 GGTAGAACCT ACCTGTCCGT TGCCTAAAAT GAGGATTTTC ACGGTGTGGG 551 TCGCCGAGTG A This corresponds to the amino acid sequence <SEQ ID 192; ORF 046. a>: a046.pep 1 MSAMLRPTSS PPRRACMMTI RTRSSAKRKT CNAPGQSIRP ASCSVTSCSG 51 LMVSVMPNME RLPFSLFSSL GLRYSRYSLE RTRAMRPGML NRSAATLQAT 101 MLVSSLRESA SSKSASSAPA RSNVKGDAPL PKTVWTSRRL PVSCNAFSSM 151 SITVTSLLGM AARFCATVEP TCPLPKMRIF TVWVAE* m046/a046 98.4%> identity over a 186 aa overlap 10 20 30 40 50 60 m046.pep MSAMLRPTSXPXXRACMMTIRTRSSAKRKTCNAPGQSIRPASCSVTSCSGLMVSVMPNME II I II I I M I II II I II I M I I I I II I II I II I II I II I II II I II II I II I II I II a046 MSAMLRPTSSPPRRACMMTIRTRSSAKRKTCNAPGQSIRPASCSVTSCSGLMVSVMPNME 10 20 30 40 50 60 70 80 90 100 110 120 m046.pep RLPFSLFSSLGLRYSRYSLERTRAMRPGMLNRSAATLQATMLVSSLRESASSKSASSAPA I I I I I I II I II II II II I II II I II II I II II II I II II II II II II II I II II I II I II a046 RLPFSLFSSLGLRYSRYSLERTRAMRPGMLNRSAATLQATMLVSSLRESASSKSASSAPA 70 80 90 100 110 120 130 140 150 160 170 180 m046. pep RSNVKGDAPLPKTVWTSRRLPVSCNAFSSMSITVTSLLGMAARFCATVEPTCPLPKMRIF II I II II II I II II I II I II I II I I I II I II II II I I II II I I II II II I II I II I I II I a046 RSNVKGDAPLPKTVWTSRRLPVSCNAFSSMSITVTSLLGMAARFCATVEPTCPLPKMRIF 130 140 150 160 170 180 m046.pep TVWVAEX l l l l l l l a046 TVWVAEX Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N. gonorrhoeae ORF 046 shows 97.3% identity over a 185 aa overlap with a predicted ORF (ORF 046.ng) from N gonorrhoeae: m046/g046 10 20 30 40 50 60 m046.pep MSAMLRPTSXPXXRACMMTIRTRSSAKRKTCΝAPGQSIRPASCSVTSCSGLMVSVMPΝME I g046 MSAMLRPTSSPPRRACMMTIRTRSSAKRKTCΝAPGQSIRPASCSVTSCSGLMVSVMPΝME 10 20 30 40 50 60 70 80 90 100 110 120 m046. pep RLPFSLFSSLGLRYSRYSLERTRAMRPGMLΝRSAATLQATMLVSSLRESASSKSASSAPA g046 RLPFSLFSSLGLRYSRYSLERTRAMRPGMLΝRSAATLQATMLVSSLRESASSKSASSAPA 70 80 90 100 110 120 130 140 150 160 170 180 m046.pep RSΝVKGDAPLPKTVWTSRRLPVSCΝAFSSMSITVTSLLGMAARFCATVEPTCPLPKMRIF I 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 g046 RYΝVKGDAPLPKTVWTSRRLPVSCΝAFSSMSITVTSLLVMAARFCATVEPTCPLPKMRIF 130 140 150 160 170 180 m046.pep TVWVAEX Mill g046 TVWVAGX The following partial DΝA sequence was identified in N. gonorrhoeae <SEQ ID 193>: g047. seq 1 ATGGTCATCA TACAGGCGcg gcGCGGCGGG CTGCTTGTCG GACGCAGCAT 51 TGCCGACATC GCCCAAGATT TGCCCGACGG GGCCGACTGC CAAATCTGCG 101 CCGTTTACCG CAACAACCGC CTCATCGTCC CCGCGCCGCA AACCGTCATC 151 ATCGAAGGCG ACGAAATCCT GTTTGCCGCC GCCGCCGAAA ACATCGGGGC 201 GGTCATACCc gaATTGCGCC CCAAAGAAAC CAGCACCCGC CGCATCATGA 251 TTGCCGGCGG CGGCAACATc tgctACCGCC TCGCCAAGCA GCTCGAACAC 301 GCATAcaacG TCAAAATCAT CGAATGCCGG CCGCGCcgtg ccgaATGGAT 351 AGCCGAAAAC ctcgAcaaCA CCCTCGTCCT GCAAGGTTCG Gcaaccgacg 401 aAaccctgct cgAcaacgaa tacatcgacg aaatcgaCGT ATTCTGCGCC 451 CTGACCAACG ACGACGAAAG CAACATTAtg tCCGCCCTTT TGGCGAAAAA 501 CCTcggcgCG AAGCgcgtca tcggCATCGT CAACCGCTCA AGCTACGTCG 551 ATTTGCTCGA AGGCAACAAA ATCGACATCG TCGTCTCCCC CCACCTCATC 601 ACCATCGGCT CGATACTCGC CCACATCCGG CGCGGCGACA TCGTTGCCGT 651 CCACCCCATC CGGCGCGGCA CGGCGGAAGC CATCGAAGTC GTCGCGCACG 701 GCGACAAAAA AACTTCCGCC ATCATCGGCA GGCGCATCAG CGGCATCAAA 751 TGGCCCGAAG GCTGCCACAT TGCCGCCGTC GTCCGCGCCG GAACCGGCGA 801 AACCATTATG GGACACCATA CCGAAACCGT CATCCAAGAC GGTGACCACA 851 TCATCTTTTT CGTCTCGCGC CGGCGCATCC TGAACGAACT GGAGAAACTC 901 ATCCAAGTCA AAATGGGCTT TTTCGGATAA This corresponds to the amino acid sequence <SEQ ID 194; ORF 047.ng>: g047.pep 1 MVIIQARRGG LLVGRSIADI AQDLPDGADC QICAVYRNNR LIVPAPQTVI 51 IEGDEILFAA AAENIGAVIP ELRPKETSTR RIMIAGGGNI CYRLAKQLEH 101 AYNVKIIECR PRRAEWIAEN LDNTLVLQGS ATDETLLDNE YIDEIDVFCA 151 LTNDDESNIM SALLAKNLGA KRVIGIVNRS SYVDLLEGNK IDIWSPHLI 201 TIGSILAHIR RGDIVAVHPI RRGTAEAIEV VAHGDKKTSA IIGRRISGIK 251 WPEGCHIAAV VRAGTGETIM GHHTETVIQD GDHIIFFVSR RRILNELEKL 301 IQVKMGFFG* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 195>: m047.seq 1 ATGGTCATCA TACAGgCGcG C .. syGCGGA STGCTTGTCG GACGCAGCAT 51 TGCCGACATC GCCCAAGATT TGCCCGACGG GGCCGACTGC CAAATCTGCG 101 CCGTTTACCG CAACAACCGC CTCATCGTCC CCGCGCCGCA AACCGTCATC 151 ATCGAAGGCG ACGAAATCCT ATTTGCCGCC GCCGCCGAAA ACATCGGCGC 201 GGTCATACCC GAATTGCGCC CCAAAGAAAC CCAAAGAAAC CAGCcCmgmm 251 GcATCATGAT TkCCGGCGGC GGCAACATCG GCTACCGTCT CGCCAAGCAG 301 CTCGAACACG CATACAACGT yAAAATCATC GAATGCCGGC CGCGCCGTGC 351 CGAATGGATA GCCGAAAACC TCGACAACAC CCTCGTCyTG CAAGGTTCGG 401 CAACCGACGA AACCCTGCTC GACAACGAAT ACATCGACGA AATCGACGTA 451 TTCTGCGCCC TGACCAACGA CGACGAAAGC AACATTATGT CCGCCCTTTT 501 GGCGAaAAAC CTCGGCGCGA AGCGCGTCAT CGGCATCGTC AACCGCTCAA 551 GCTACGTCGA TTTGCTCGAA GGCAACAAAA TCGACATCGT CGTCTCCCCC 601 CACCTCATCA CCATCGGCTC GATACTCGCC CACATCCGGC GCGGCGACAT 651 CGTTGCCGTC CACCCCATCC GGCGCGGCAC GGCGGAAGCC ATCGAAGTCG 701 TCGCACACGG CGACAAAAAA ACTTCCGCCA TCATCGGCAG GCGCATCAGC 751 GGCATCAAAT GGCCCGAAGG CTGCCACATT GCCGCCGTCG TCCGCGCCGG 801 AACCGGCGAA ACCATTATGG GACACCATAC CGAAACCGTC ATCCAAGACG 851 GCGACCACAT CATCTTTTTC GTCTCGCGCC GGCGCATCCT GAACGAACTG 901 GAAAAACTCA TCCAGGTCAA AATGGGCTTT TTCGGATAA This corresponds to the amino acid sequence <SEQ ID 196; ORF 047>: m047.pep 1 MVIIQARXXG XLVGRSIADI AQDLPDGADC QICAVYRΝΝR LIVPAPQTVI 51 IEGDEILFAA AAENIGAVIP ELRPKETQRN QPXXIMIXGG GNIGYRLAKQ 101 LEHAYNVKII ECRPRRAEWI AENLDNTLVL QGSATDETLL DNEYIDEIDV 151 FCALTNDDES NIMSALLAKN LGAKRVIGIV NRSSYVDLLE GNKIDIWSP 201 HLITIGSILA HIRRGDIVAV HPIRRGTAEA IEWAHGDKK TSAIIGRRIS 251 GIKWPEGCHI AAWRAGTGE TIMGHHTETV IQDGDHIIFF VSRRRILNEL 301 EKLIQVKMGF FG* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 197>: a047. seq 1 ATGGTCATCA TACAGGCGCG GCGCGGCGGA CTGCTTGTCG GACGCAGCAT 51 TGCCGACATC GCCCAAGATT TGCCCGACGG GGCCGACTGC CAAATCTGCG 101 CCGTTTACCG CAACAACCGC CTCATCGTCC CCGCGCCGCA AACCGTCATC 151 ATCGAAGGCG ACGAAATCCT ATTTGCCGCC GCCGCCGAAA ACATCGGCGC 201 GGTCATACCC GAATTGCGCC CCAAAGAAAC CAGCACCCGC CGCATCATGA 251 TTGCCGGCGG CGGCAACATC GGCTACCGTC TCGCCAAGCA GCTCGAACAC 301 GCATACAACG TCAAAATCAT CGAATGCCGG CCGCGCCGTG CCGAATGGAT 351 AGCCGAAAAC CTCGACAACA CCCTCGTCCT GCAAGGTTCG GCAACCGACG 401 AAACCCTGCT CGACAACGAA TACATCGACG AAATCGACGT ATTCTGCGCC 451 CTGACCAACG ACGACGAAAG CAACATTATG TCCGCCCTTT TGGCGAAAAA 501 CCTCGGCGCG AAGCGCGTCA TCGGCATCGT CAACCGCTCA AGCTACGTCG 551 ATTTGCTCGA AGGCAACAAA ATCGACATCG TCGTCTCCCC CCACCTCATC 601 ACCATCGGCT CGATACTCGC CCACATCCGG CGCGGCGACA TCGTTGCCGT 651 CCACCCCATC CGGCGCGGCA CGGCGGAAGC CATCGAAGTC GTCGCACACG 701 GCGACAAAAA AACTTCCGCC ATCATCGGCA GGCGCATCAG CGGCATCAAA 751 TGGCCCGAAG GCTGCCACAT TGCCGCCGTC GTCCGCGCCG GAACCGGCGA 801 AACCATTATG GGACACCATA CCGAAACCGT CATCCAAGAC GGCGACCACA 851 TCATCTTTTT CGTCTCGCGC CGGCGCATCC TGAACGAACT GGAAAAACTC 901 ATCCAAGTCA AAATGGGCTT TTTCGGATAA This corresponds to the amino acid sequence <SEQ ID 198; ORF 047. a>: a047.pep 1 MVIIQARRGG LLVGRSIADI AQDLPDGADC QICAVYRNNR LIVPAPQTVI 51 IEGDEILFAA AAENIGAVIP ELRPKETSTR RIMIAGGGNI GYRLAKQLEH 101 AYNVKIIECR PRRAEWIAEN LDNTLVLQGS ATDETLLDNE YIDEIDVFCA 151 LTNDDESNIM SALLAKNLGA KRVIGIVNRS SYVDLLEGNK IDIWSPHLI 201 TIGSILAHIR RGDIVAVHPI RRGTAEAIEV VAHGDKKTSA IIGRRISGIK 251 WPEGCHIAAV VRAGTGETIM GHHTETVIQD GDHIIFFVSR RRILNELEKL 301 IQVKMGFFG* m047/a047 96.5%> identity over a 312 aa overlap 10 20 30 40 50 60 m047.pep MVIIQARXXGXLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEILFAA lllllll I I I II II II II II II II II II II II II II II I I II I II I II I I II II II I a047 MVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEILFAA 10 20 30 40 50 60 70 80 90 100 110 120 m047.pep AAENIGAVIPELRPKETQRNQPXXIMIXGGGNIGYRLAKQLEHAYNVKIIECRPRRAEWI II II II II I II I II II I : : III II II II II I II II II I II II I I II II II II II a047 AAENIGAVIPELRPKETSTRR IMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAEWI 70 80 90 100 110 130 140 150 160 170 180 m047.pep AENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRVIGIV I I I II II II I II I I II I I II II II I I II II I II II II II II I II I I II I II II II II II I a047 AENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRVIGIV 120 130 140 150 160 170 190 200 210 220 230 240 m047.pep NRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEWAHGDKK II II I I II I II II II II II I II I II II I II I II II II II I II I II II I I II II II II II I a047 NRSSYVDLLEGNKIDIWSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEWAHGDKK 180 190 200 210 220 230 250 260 270 280 290 300 m047.pep TSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVSRRRILNEL II II I II II II I II II I I II I II II I II I II I I I II II II II II II I I II I I II II II II a047 TSAIIGRRISGIKWPEGCHIAAWRAGTGETIMGHHTETVIQDGDHIIFFVSRRRILNEL 240 250 260 270 280 290 310 m047.pep EKLIQVKMGFFGX II II I II II I II I a047 EKLIQVKMGFFGX 300 310 Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N. gonorrhoeae ORF 047 shows 96.2% identity over a 312 aa overlap with a predicted ORF (ORF 047.ng) from N gonorrhoeae: m047/g045 m047.pep MVIIQARXXGXLVGRSIADIAQDLPDGADCQICAVYRΝΝRLIVPAPQTVIIEGDEILFAA 60 Mlllll I g047 MVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRΝΝRLIVPAPQTVIIEGDEILFAA 60 m047.pep AAEΝIGAVIPELRPKETQRΝQPXXIMIXGGGΝIGYRLAKQLEHAYΝVKIIECRPRRAEWI 120 MIIIMMMMIII = III Mill 11 II 111 II II II I 11 II 11 II II 11 g047 AAENIGAVIPELRPKETSTRR IMIAGGGNICYRLAKQLEHAYNVKIIECRPRRAEWI 117 m047.pep AENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRVIGIV 180 MMMMMMMMMIMMMMMMMMMMMMMIIMIMMMMM g047 AENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRVIGIV 177 m047.pep NRSSYVDLLEGNKIDIWSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEWAHGDKK 240 MMMMMMMMMMMMMMMMMMIMMMMIIIMMMMMM g047 NRSSYVDLLEGNKIDIWSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEWAHGDKK 237 m047.pep TSAIIGRRISGIKWPEGCHIAAWRAGTGETIMGHHTETVIQDGDHIIFFVSRRRILNEL 300 MMMMMMMMMMMMMMMMMMMMMMMIIIMMIMMM g047 TSAIIGRRISGIKWPEGCHIAAWRAGTGETIMGHHTETVIQDGDHIIFFVSRRRILNEL 297 m047.pep EKLIQVKMGFFGX 313 MMIMMMM g047 EKLIQVKMGFFGX 310 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 199>: g048. seq 1 ATGCTCGACA AAGGCGAGGA GTTGCCCGTC GATTTCACCA ACCGCCTGAT 51 TTACTACGTc ggcCCcgTCG ATCCGGTCGG CGATGAAGTC GTCGGTCCCG 101 CAGGTCCGAC CACAGCCACC CGCATGGACA AATTTACCCG CCAAATGCTC 151 AAACAAACCG GCCTCTTGGG CATGATCGGC AAATCCGagc gcgGcgcggc 201 cacctGCGAA GCcatCGCCG ACAACAAGGC CGTGTACCTC ATGGCAGTCG 251 GCGGCGCGGC ATACCTCGTG GCAAAAGCCA TCAAATCTTC CAAAGTCTTG 301 GCGTTCCCCG AATTGGGTAT GGAAGCCGTT TACGAATTTG AAGTCAAAGA 351 TATGCCCGTA ACCGTCGCCG TGGACAGCAA AGGCGAATCC ATCCACGCCA 401 CCGCCCCGCG CAAATGGCAG GCGAAAATCG GCATCATCCC CGTCGAGTCT 451 TGA This corresponds to the amino acid sequence <SEQ ID 200; ORF 048. ng>: g048.pep 1 MLDKGEELPV DFTΝRLIYYV GPVDPVGDEV VGPAGPTTAT RMDKFTRQML 51 KQTGLLGMIG KSERGAATCE AIADΝKAVYL MAVGGAAYLV AKAIKSSKVL 101 AFPELGMEAV YEFEVKDMPV TVAVDSKGES IHATAPRKWQ AKIGIIPVES 151 * The following partial DΝA sequence was identified in N meningitidis <SEQ ID 201>: m048. seq 1 ATGCTCAACA AAGGCGAAGA ATTGCCCGTC GATTTCACCA ACCGCCTGAT 51 TTACTACGTC GGCCCCGTCG ATCCGGTCGG CGATGAAGTC GTCGGTCCGG 101 CAGGTCCGAC CACAGCCACC CGCATGGACA AATTCACCCG CCAAATGCTC 151 GAACAAACCG ACCTCTTGGG CATGATCGGC AAATCCGAGC GCGGCGTGGC 201 CACCTGCGAA GCCATCGCCG ACAACAAAGC CGTGTACCTC ATGGCAGTCG 251 GCGGCGCGGC GTATCTCGTG GCAAAAGCCA TCAAATCTTC CAAAGTCTTG 301 GCGTTCCCCG AATTGGGCAT GGAAGCCATT TACGAATTTG AAGTCAAAGA 351 CATGCCCGTA ACCGTCGCCG TAGATAGCAA AGGCGAATCC ATCCACGCCA 401 CCGCCCCGCG CAAATGGCAG GCGAAAATCG GCATCATCCC CGTCGAATCT 451 TGA This corresponds to the amino acid sequence <SEQ ID 202; ORF 048>: m048.pep 1 MLNKGEELPV DFTNRLIYYV GPVDPVGDEV VGPAGPTTAT RMDKFTRQML 51 EQTDLLGMIG KSERGVATCE AIADNKAVYL MAVGGAAYLV AKAIKSSKVL 101 AFPELGMEAI YEFEVKDMPV TVAVDSKGES IHATAPRKWQ AKIGIIPVES 151 * The following partial DNA sequence was identified in N meningitidis <SEQ ID 203>: a048.seq 1 ATGCTCGACA AAGGCGAAGA ATTGCCCGTC GATTTCACCA ACCGCCTGAT 51 TTACTACGTC GGCCCCGTCG ATCCGGTCGG CGACGAAATC GTCGGCCCAG 101 CAGGTCCGAC CACCGCCACC CGCATGGACA AATTCACCCG CCAAATGCTC 151 GAACAAACCG ACCTCTTGGG CATGATCGGC AAATCCGAGC GCGGCGCGGC 201 CACCTGCGAA GCCATCGCCG ACAACAAAGC CGTGTACCTC ATGGCAGTCG 251 GCGGCGCGGC GTATCTCGTG GCAAAAGCCA TCAAATCTTC CAAAGTCTTG 301 GCGTTCCCCG AATTGGGCAT GGAAGCCATT TACGAATTTG AAGTCAAAGA 351 CATGCCCGTA ACCGTCGCCG TAGACAGCAA AGGCGAATCC ATCCACGCCA 401 CCGCCCCGCC CCAATGGCAG GCGAAAATCG GCATCATCCC CGTCAAATCT 451 TGA This corresponds to the amino acid sequence <SEQ ID 204; ORF 048.a>: a048.pep 1 MLDKGEELPV DFTNRLIYYV GPVDPVGDEI VGPAGPTTAT RMDKFTRQML 51 EQTDLLGMIG KSERGAATCE AIADNKAVYL MAVGGAAYLV AKAIKSSKVL 101 AFPELGMEAI YEFEVKDMPV TVAVDSKGES IHATAPPQWQ AKIGIIPVKS 151 * m048/a048 96.0%o identity over a 150 aa overlap 10 20 30 40 50 60 mO 48 . pep MLNKGEELPVDFTNRLI YYVGPVDPVGDEWGPAGPTTATRMDKFTRQMLEQTDLLGMIG I I : I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a048 MLDKGEELPVDFTNRLIYYVGPVDPVGDEIVGPAGPTTATRMDKFTRQMLEQTDLLGMIG 10 20 30 40 50 60 70 80 90 100 110 120 m048. pep KSERGVATCEAIADNKAVYLMAVGGAAYLVAKAIKSSKVLAFPELGMEAIYEFEVKDMPV I I II I : I II I II II I II I II I II II I I II II II II I II II II I I II I I I I II I II I I II I a048 KSERGAATCEAIADNKAVYLMAVGGAAYLVAKAIKSSKVLAFPELGMEAIYEFEVKDMPV 70 80 90 100 110 120 130 140 150 m048.pep TVAVDSKGESIHATAPRKWQAKIGIIPVESX I II II II I II II I II I : I II I II II I I : II a048 TVAVDSKGESIHATAPPQWQAKIGIIPVKSX 130 140 150 Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 048 shows 96.4% identity over a 150 aa overlap with a predicted ORF (ORF 048.ng) from N gonorrhoeae: m048/g048 10 20 30 40 50 60 m048. pep MLNKGEELPVDFTNRLIYYVGPVDPVGDEWGPAGPTTATRMDKFTRQMLEQTDLLGMIG IMMI g048 MLDKGEELPVDFTNRLIYYVGPVDPVGDEWGPAGPTTATRMDKFTRQMLKQTGLLGMIG 10 20 30 40 50 60 70 80 90 100 110 120 m048.pep KSERGVATCEAIADNKAVYLMAVGGAAYLVAKAIKSSKVLAFPELGMEAIYEFEVKDMPV MIMMIIIMIIIMIIIIMIIMMIIIIMIIMIMIIIIMhllllMIIII g048 KSERGAATCEAIADNKAVYLMAVGGAAYLVAKAIKSSKVLAFPELGMEAVYEFEVKDMPV 70 80 90 100 110 120 130 140 150 m048.pep TVAVDSKGESIHATAPRKWQAKIGIIPVESX MMMMMMMMMMMMMMMI g048 TVAVDSKGESIHATAPRKWQAKIGIIPVESX 130 140 150 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 205>: g049. seq 1 ATGCGGGCGC AGGCGTTTGA TCAACCGTTC GGTCAGCTCC TGTTCGGACA 51 GGCAGAACAC TTCGCGCCGG TTGACGGCTT TCGGGTTCAG GATATTGATT 101 TGGACGGGCA TCAACGCCTC TTCCGCACCG CCTTCGCCGT TTTCCGCAAC 151 CCCGTCTGCC GCCGTACCGG ATTCTGCCGC ATCGGCGTTT TCCCCGCCCT 201 CAATCTGTGC GGTTTCAAAT TCGGCACTGT CTTTTTTGGC ATCGAACCGG 251 ATTCTCCGCC GCGATTCGAT GTGTTTTTCC GAAAccggca tTTGCAGGGA 301 AGCCTgcgcg TTGAGCCAGT TTTCCTGAAG GACGATCATC GGGTCGGTTT 351 CGACTTCCTC GCCGCAATCG GCAACGGCgc tGTTGTGTTC TTCCTGCCAT 401 TTCTTCAGAT ACGCCTTTAA This corresponds to the amino acid sequence <SEQ ID 206; ORF 049.ng>: g049.pep 1 MRAQAFDQPF GQLLFGQAEH FAPVDGFRVQ DIDLDGHQRL FRTAFAVFRΝ 51 PVCRRTGFCR IGVFPALΝLC GFKFGTVFFG IEPDSPPRFD VFFRΝRHLQG 101 SLRVEPVFLK DDHRVGFDFL AAIGΝGA F FLPFLQIRL* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 207>: m049.seq (partial) 1 ATGCGGGCGC AGGCGTTTGA TCAGCCGTTC GGTCAGCTCC TGTTCGGACA 51 GGCAGAACAC TTCGCGCCGG TTGACGGCTT TCGGGTTCAG GATATTGATT 101 TGGACGGGCA TCAACGTTTC TTCCGCATCG TTTTCCCCGT TTTCCGAAAC 151 CGCCGGCTCA TTCGTGCCGG ATTCTGCCTC GTCGGCGTTT TCCCCGCTTT 201 CAATCTGTCC GGTTTCAAAT TCGACACTGT CTTTTTTGGT ATCAAACCGG 251 ATTCTCCGCC GCGATTCGAT GTGTTTTTCC GAAACCGACA TTTGCAGGGA 301 AGCCTGCGCG TTGAGCCAGT TTTCCTGAAG GACGATCATC GGGTCGGTTT 351 CGACTTCCTC GCCGCAATCG GCAACGGCGG CATTGTGTTC CTCCTGCCAT 401 TTTTTCAGAT ACGCCTT ... This corresponds to the amino acid sequence <SEQ ID 208; ORF 049>: m049.pep (partial) 1 MRAQAFDQPF GQLLFGQAEH FAPVDGFRVQ DIDLDGHQRF FRIVFPVFRΝ 51 RRLIRAGFCL VGVFPAFΝLS GFKFDTVFFG IKPDSPPRFD VFFRΝRHLQG 101 SLRVEPVFLK DDHRVGFDFL AAIGΝGGIVF LLPFFQIRL ... The following partial DΝA sequence was identified in N meningitidis <SEQ ID 209>: a049. seq 1 ATGCGGGCGC AGGCGTTTGA TCAGCCGTTC GGTCAGCTCC TGTTCGGACA 51 GGCAGAACAC TTCGCGCCGG TTGACGGCTT TCGGGTTCAG AATATTGATT 101 TGGACGGGCA TCAACGCTTC TTCCGCACCG CCTTCGCCGT TTTCCGCAAC 151 CCCGTCTGCC GCCGTACCCG ATTCTGCCGC ATCGGCGTTT TCCCCGCCTT 201 CAATCTGTCC GGTTTCAAAT TCGGCACTGT CTTTTTTGGC ATCAAACCGG 251 ATTCTCCGCC GCGATTCGAT GTGTTTTTCC GAAACCGACA TTTGCAGGGA 301 AGCCTGCGCG TTGAGCCAGT TTTCCTGAAG GACGATCATC GGGTCGGTTT 351 CGACTTCCTC GCCGCAATCG GCAACGGCGG CATTGTGTTC CTCCTGCCAT 401 TTTTTCAGAT ACGCCTT This corresponds to the amino acid sequence <SEQ ID 210; ORF 049.a>: a049.pep 1 MRAQAFDQPF GQLLFGQAEH FAPVDGFRVQ NIDLDGHQRF FRTAFAVFRN 51 PVCRRTRFCR IGVFPAFNLS GFKFGTVFFG IKPDSPPRFD VFFRNRHLQG 101 SLRVEPVFLK DDHRVGFDFL AAIGNGGIVF LLPFFQIRL m049/a049 90.6%o identity over a 139 aa overlap 10 20 30 40 50 60 m049. pep MRAQAFDQPFGQLLFGQAEHFAPVDGFRVQDIDLDGHQRFFRIVFPVFRNRRLIRAGFCL II II II II I II I I II I I I II II II II II II : I II II II II I I : I I I II I : II a049 MRAQAFDQPFGQLLFGQAEHFAPVDGFRVQNIDLDGHQRFFRTAFAVFRNPVCRRTRFCR 10 20 30 40 50 60 70 80 90 100 110 120 m049.pep VGVFPAFNLSGFKFDTVFFGIKPDSPPRFDVFFRNRHLQGSLRVEPVFLKDDHRVGFDFL : I II II II I II II I II II II I II II II I II II II I I I II II I II II I II I I II II I II I a049 IGVFPAFNLSGFKFGTVFFGIKPDSPPRFDVFFRNRHLQGSLRVEPVFLKDDHRVGFDFL 70 80 90 100 110 120 130 139 m049.pep AAIGNGGIVFLLPFFQIRL I II II II II I II I II I II I a049 AAIGNGGIVFLLPFFQIRL 130 Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 049 shows 86.3% identity over a 139 aa overlap with a predicted ORF (ORF 049.ng) from N gonorrhoeae: m049/g049 10 20 30 40 50 60 m049. pep MRAQAFDQPFGQLLFGQAEHFAPVDGFRVQDIDLDGHQRFFRIVFPVFRΝRRLIRAGFCL MMMMMMMMMMMMMMMMIMMMMI M MM IMM g049 MRAQAFDQPFGQLLFGQAEHFAPVDGFRVQDIDLDGHQRLFRTAFAVFRΝPVCRRTGFCR 10 20 30 40 50 60 70 80 90 100 110 120 m049.pep VGVFPAFΝLSGFKFDTVFFGIKPDSPPRFDVFFRΝRHLQGSLRVEPVFLKDDHRVGFDFL M I I I I M I M M M M M M M M M M M M M M M M M M M M M I M M M g049 IGVFPALΝLCGFKFGTVFFGIEPDSPPRFDVFFRΝRHLQGSLRVEPVFLKDDHRVGFDFL 70 80 90 100 110 120 130 139 m049.pep AAIGNGGIVFLLPFFQIRL M l l l h M I M M M I M g049 AAIGNGAWFFLPFLQIRLX 130 140 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 211>: g050.seq 1 atgggcgCGG GCTGGTGTCC TCCCGGCATC TTGGGCATCG GCATCGGCGg 51 cacgcccGAA AAAGccgtgt TGATGGcaaA AGAATCCCTG ATGAGCCACA 101 TCGAcatCca aGaATTGCAG GAAAAAGCCG CGTccggggc ggaattgtcc 151 accaccgaAG ccCTGCGCCT cGAACTCTTT GAAAAGGTCA ACGCGCTGGG 201 CATCGGCGCG CAAGGCTTGG GCGGTCTGAC CACCGTGTTG GACGTGAAAA 251 TCCTCGATTA CCCGACCCAT GCCGCCTCCA AACCGATTGC CATGATTCCC 301 AACTGTGCcg ccacCCGcca cgtcgAATTT GAATTGgACG GCTCAGGtcc 351 TGTCGAactc acgccGCcgc gtgtCGAAGA CTGA This corresponds to the amino acid sequence <SEQ ID 212; ORF 050.ng>: g050.pep 1 MGAGWCPPGI LGIGIGGTPE KAVLMAKESL MSHIDIQELQ EKAASGAELS 51 TTEALRLELF EKVΝALGIGA QGLGGLTTVL DVKILDYPTH AASKPIAMIP 101 ΝCAATRHVEF ELDGSGPVEL TPPRVED* The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 213>: m050.seq 1 ATGGGCGCGG GCTGGTGTCC TCCCGGCATC TTGGGTATCG GCATCGGCGG 51 C..agCCgAA AAAGCCGTGC TGATGGCAAA AGAGTCCCTG ATGAGCCACA 101 TCGACATTCA AGAATTGCAG GAAAAGGCCG CGTCCGGCGC GgAATTGTCC 151 ACCACCGAAG CCCTGCGCCT CGAACTCTTT GAAAAAGTCA ACGCGCTGGG 201 CATCGGCGCA CAAGGCTTGG GCGGACTGAC CACCGTGTTG GACGTGAAAA 251 TCCTCGATTA TCCGACCCAC GCCGCCTCCA AACCGATTGC CATGATTCCG 301 AACTGCGCCG CCACCCGCCA CGTCGAATTT GAATTGGACG GCTCAGGCCC 351 TGTCGAACTC ACGCCGCCGC GCGTCGAAGA TGGCCCGATT TGA This corresponds to the amino acid sequence <SEQ ID 214; ORF 050>: m050.pep 1 MGAGWCPPGI LGIGIGGXAE KAVLMAKESL MSHIDIQELQ EKAASGAELS 51 TTEALRLELF EKVΝALGIGA QGLGGLTTVL DVKILDYPTH AASKPIAMIP 101 ΝCAATRHVEF ELDGSGPVEL TPPRVEDGPI * The following partial DΝA sequence was identified in N meningitidis <SEQ ID 215>: a050. seq 1 ATGGGCGCGG GCTGGTGTCC TCCCGGCATC TTGGGCATCG GCATCGGCGG 51 TACGCCCGAA AAAGCCGTGT TGATGGCGAA AGAATCCCTG ATGAGCCACA 101 TCGACATCCA AGAATTGCAG GAAAAAGCCG CGTCCGGCGC GGAATTGTCC 151 ACCACCGAAG CCCTGCGCCT CGAACTCTTT GAAAAAGTCA ACGCGCTAGG 201 CATCGGCGCG CAAGGCTTGG GCGGTCTGAC CACCGTGTTG GACGTGAAAA 251 TCCTCGATTA CCCGACCCAC GCCGCCTCCA AACCGATTGC CATGATTCCG 301 AACTGCGCCG CCACCCGCCA CGTCGAATTT GAATTGGACG GCTCAGGCCC 351 TGTCGAACTC ACGCCGCCGC GCGTCGAAGA CTGGCCC This corresponds to the amino acid sequence <SEQ ID 216; ORF 050.a>: a050.pep 1 MGAGWCPPGI LGIGIGGTPE KAVLMAKESL MSHIDIQELQ EKAASGAELS 51 TTEALRLELF EKVΝALGIGA QGLGGLTTVL DVKILDYPTH AASKPIAMIP 101 ΝCAATRHVEF ELDGSGPVEL TPPRVEDWP moso/aoso 91.1% identity over a 129 aa overlap 10 20 30 40 50 60 m050. pep MGAGWCPPGILGIGIGGXAEKAVLMAKESLMSHIDIQELQEKAASGAELSTTEALRLELF II I II II I II M II I I I: I II I II I II I II I II II II I I I II II I II II I II II II II I a050 MGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQEKAASGAELSTTEALRLELF 10 20 30 40 50 60 70 80 90 100 110 120 m050. pep EKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAMIPNCAATRHVEFELDGSGPVEL I I I II I I I II I II II II I II I II II I II I II II I II II II II II I II II I I II I II I II I a050 EKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAMIPNCAATRHVEFELDGSGPVEL 70 80 90 100 110 120 130 m050.pep TPPRVEDGPIX lllllll I a050 TPPRVEDWP Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 050 shows 98.4% identity over a 127 aa overlap with a predicted ORF (ORF 050.ng) from N gonorrhoeae: m050/g050 10 20 30 40 50 60 m050.pep MGAGWCPPGILGIGIGGXAEKAVLMAKESLMSHIDIQELQEKAASGAELSTTEALRLELF M M M M I M M M I h I I I I I I I I I I 1 1 1 I I 1 1 I I 1 1 1 1 1 I I I I I I I I M I I I 1 1 I I g050 MGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQEKAASGAELSTTEALRLELF 10 20 30 40 50 60 70 80 90 100 110 120 m050.pep EKVΝALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAMIPΝCAATRHVEFELDGSGPVEL MMMMMIMMMMMMMMMMMMMIMMMMMMMMMMM g050 EKVΝALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAMIPΝCAATRHVEFELDGSGPVEL 70 80 90 100 110 120 130 m050.pep TPPRVEDGPIX g050 TPPRVEDX The following partial DΝA sequence was identified in N. gonorrhoeae <SEQ ID 217>: g050-l.seq 1 ATGACCGTTA TCAAGCAAGA AGACTTTATT CAAAGTATCT GCGATGCCTT 51 CCAATTCATC AGCTACTACC ATCCAAAAGA CTACATCGAC GCGCTTTATA 101 AGGCGTGGCA GAAGGAAGAA AATCCCGCCG CCAAAGACGC GATGACGCAG 151 ATTTTGGTCA ACAGCCGTAT GTGTGCCGAA AACAACCGCC CCATCTGCCA 201 AGACACAGGT ATCGCAACCG TCTTCCTCAA AGTCGGTATG GATGTGCAAT 251 GGGATGCGGA CATGAGCGTG GAAAAGATGG TTAACGAAGG CGTACGCCGC 301 GCCTACACTT GGGAAGGCAA CACCCTGCGC GCTTCCGTCC TCGCCGATCC 351 GGCCGGCAAA CGCCAAAACA CCAAAGACAA CACCCCCGCC GTCATCCACA 401 TGAGCATCGT GCCGGGCGGT AAAGTCGAAG TAACCTGCGC GGCAAAAGGC 451 GGCGGCTCTG AAAACAAATC CAAACTCGCT ATGCTCAACC CTTCCGACAA 501 CATCGTCGAT TGGGTATTGA AAACCATCCC GACGATGGGC GCGGGCTGGT 551 GTCCTCCCGG CATCTTGGGC ATCGGCATCG GCGGCAcgcC CGAAAAAGCC 601 GTGTTGATGG cgaAAGAATC CCTGATGAGC CACATCGACA TCCAAGAATT 651 GCAGGAAAAA GCCGCGTCCG GCGCGGAATT GTCCACCACC GAAGCCCTGC 701 GCCTCGAACT CTTTGAAAAG GTCAACGCGC TGGGCATCGG CGCGCAAGGC 751 TTGGGCGGTC TGACCACCGT GTTGGACGTG AAAATCCTCG ATTACCCGAC 801 CCATGCCGCC TCCAAACCGA TTGCCATGAT TCCCAACTGT GCCGCCACCC 851 GCCACGTCGA ATTTGAATTG GACGGCTCAG GTCCTGTCGA ACTCACGCCG 901 CCGCGCGTCG AAGACTGACC CGATCTGACT TACAGCCCCG ACAACGGCAA 951 ACGCGTCGAT GTCGATAAGC TGACCAAAGA AGAAGTGGCA AGCTGGAAAA 1001 CCGGCGACGT ATTGCTGTTG AACGGCAAAA TCCTCACCGG CCGCGATGCC 1051 GCGCACAAAC GCCTCGTCAA TATGCTCGAC AAAGGCGAGG AGTTGCCCGT 1101 CGATTTCACC AACCGCCTGA TTTACTACGT CGGCCCCGTC GATCCGGTCG 1151 GCGATGAAGT CGTCGGTCCC GCAGGTCCGA CCACAGCCAC CCGCATGGAC 1201 AAATTTACCC GCCAAATGCT CAAACAAACC GGCCTCTTGG GCATGATCGG 1251 CAAATCCGAG CGCGGCGCGG CCACCTGCGA AGCCATCGCC GACAACAAGG 1301 CCGTGTACCT CATGGCAGTC GGCGGCGCGG CATACCTCGT GGCAAAAGCC 1351 ATCAAATCTT CCAAAGTCTT GGCGTTCCCC GAATTGGGTA TGGAAGCCGT 1401 TTACGAATTT GAAGTCAAAG ATATGCCCGT AACCGTCGCC GTGGACAGCA 1451 AAGGCGAATC CATCCACGCC ACCGCCCCGC GCAAATGGCA GGCGAAAATC 1501 GGCATCATCC CCGTCGAGTC TTGA This corresponds to the amino acid sequence <SEQ ID 218; ORF 050-l.ng>: g050-l.pep 1 MTVIKQEDFI QSICDAFQFI SYYHPKDYID ALYKAWQKEE NPAAKDAMTQ 51 ILVNSRMCAE NNRPICQDTG IATVFLKVGM DVQWDADMSV EKMVNEGVRR 101 AYTWEGNTLR ASVLADPAGK RQNTKDNTPA VIHMSIVPGG KVEVTCAAKG 151 GGSENKSKLA MLNPSDNIVD WVLKTIPTMG AGWCPPGILG IGIGGTPEKA 201 VLMAKESLMS HIDIQELQEK AASGAELSTT EALRLELFEK VNALGIGAQG 251 LGGLTTVLDV KILDYPTHAA ΞKPIAMIPNC AATRHVEFEL DGSGPVELTP 301 PRVED*PDLT YSPDNGKRVD VDKLTKEEVA SWKTGDVLLL NGKILTGRDA 351 AHKRLVNMLD KGEELPVDFT NRLIYYVGPV DPVGDEVVGP AGPTTATRMD 401 KFTRQMLKQT GLLGMIGKSE RGAATCEAIA DNKAVYLMAV GGAAYLVAKA 451 IKSSKVLAFP ELGMEAVYEF EVKDMPVTVA VDSKGESIHA TAPRKWQAKI 501 GIIPVES* g050-l/pl4407 sp|P14407|FUMB_ECOLI FUMARATE HYDRATASE CLASS I, ANAEROBIC (FUMARASE) >gι|280063|pιr| IB44511 fumarate hydratase (EC 4.2.1.2) fumB, iron-dependent - Escherichia coli >gι|146048 (M27058) anaerobic class I fumarase (EC 4.2.1.2) [Escherichia coli] Length = 548 Score = 172 bits (432), Expect = 4e-42 Identities = 138/488 (28%), Positives = 216/488 (43%), Gaps = 22/488 (4%) Query: 11 QSICDAFQFISYYHPKDYIDALYKAWQKEENPAAKDAMTQILVNSRMCAENNRPICQDTG 70 Q+ DA + H K L+ E + K Q L NS + A+ P CQDTG Sbjct: 53 QAFHDASFMLRPAHQKQVAAILHDPEASEND KYVALQFLRNSEIAAKGVLPTCQDTG 109 Query: 71 IATVFLKVGMDVQWDADMSVEKMVNEGVRRAYTWEGNTLRASVLADPAGKRQNTKDNTPA 130 A + K G V W E+ +++GV Y E N + A K T N PA Sbjct: 110 TAIIVGKKGQRV-WTGGGD-EETLSKGVYNTYI-EDNLRYSQNAALDMYKEVNTGTNLPA 166 Query: 131 VIHMSIVPGGKVEVTCAAKGGGSENKΞKL AMLNPSDNIVDWVLKTIPTMGAGWCP 185 I + V G + + C AKGGGS NK+ L A+L P + +++++ + T+G CP Sbjct: 167 QIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPG-KLKNFLVEKMRTLGTAACP 225 Query: 186 PXXXXXXXXXTPEKAVLMAKESLMSHIDIQELQEKAAΞGAELSTTEALRLELFEKVNXXX 245 P T + L + +H EL + + L EL E+ Sbjct: 226 PYHIAFVIGGTSAETNLKTVKLASAHY-YDELPTEGNEHGQAFRDVQLEQELLEEAQKLG 284 Query: 246 XXXXXXXXXTTVLDVKILDYPTHAASKPIAMIPNCAATRHVEFELDGSG PVELTPP 301 D++++ P H AS P+ M +C+A R+++ +++ G +E P Sbjct: 285 LGAQFGGKYFAH-DIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIWIEKLEHNPG 343 uery: 302 RVEDXPDLTYSPDNGKRVDVDKLTKE EVASWKTGDVLLLNGKILTGRDAAHKRLVNM 358 + +VD+++ KE +++ + L L G 1+ GRD AH +L + Sbjct: 344 QYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVGRDIAHAKLKEL 403 Query: 359 LDKGEELPVDFTNRLIYYXXXXXXXXXXXXXXXXXTTATRMDKFTRQMLKQTGLLGMIGK 418 +D G+ELP + IYY TTA RMD + + G + M+ K Sbjct: 404 IDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDLLQSHGGΞMIMLAK 463 Query: 419 SERGAATCEAIADNKAVYLMAVGG-AAYLVAKAIKSSKVLAFPELGMEAVYEFEVKDMPV 477 R +A + YL ++GG AA L ++IK + +A+PELGMEA+++ EV+D P Sbjct: 464 GNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPELGMEAIWKIEVEDFPA 523 Query: 478 TVAVDSKG 485 + VD KG Sbjct: 524 FILVDDKG 531 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 219>: 1 ATGACCGTCA TCAAACAGGA AGACTTTATC CAAAGCATTT GCGATGCCTT 51 CCAATTCATC AGCTACTATC ATCCCAAAGA CTACATCGAC GCGCTTTATA 101 AGGCGTGGCA GAAGGAAGAA AATCCTGCCG CCAAAGACGC GATGACGCAG 151 ATTTTGGTCA ACAGCCGTAT GTGTGCGGAA AACAACCGCC CCATCTGCCA 201 AGACACAGGT ATCGCAACCG TCTTCCTCAA AGTCGGTATG AACGTCCAAT 251 GGGATGCGGA CATGAGCGTG GAAGAGATGG TTAACGAAGG CGTACGCCGC 301 GCCTACACTT GGGAAGGCAA TACGCTGCGC GCTTCCGTCC TCGCCGATCC 351 GGCCGGCAAA CGCCAAAACA CCAAAGACAA CACCCCCGCC GTCATCCATA 401 TGAGCATCGT GCCGGGCGGT AAAGTCGAAG TAACCTGCGC GGCAAAAGGC 451 GGCGGCTCTG AAAACAAATC CAAACTCGCC ATGCTCAATC CTTCCGACAA 501 CATCGTCGAT TGGGTATTGA AAACCATCCC GACCATGGGC GCGGGCTGGT 551 GTCCTCCCGG CATCTTGGGT ATCGGCATCG GCGGCACGCC CGAAAAAGCC 601 GTGCTGATGG CAAAAGAGTC CCTGATGAGC CACATCGACA TTCAAGAATT 651 GCAGGAAAAG GCCGCGTCCG GCGCGGAATT GTCCACCACC GAAGCCCTGC 701 GCCTCGAACT CTTTGAAAAA GTCAACGCGC TGGGCATCGG CGCACAAGGC 751 TTGGGCGGAC TGACCACCGT GTTGGACGTG AAAATCCTCG ATTATCCGAC 801 CCACGCCGCC TCCAAACCGA TTGCCATGAT TCCGAACTGC GCCGCCACCC 851 GCCACGTCGA ATTTGAATTG GACGGCTCAG GCCCTGTCGA ACTCACGCCG 901 CCGCGCGTCG AAGACTGGCC CGATTTGACT TACAGCCCCG ACAACGGCAA 951 ACGCGTCGAT GTCGACAAGC TGACCAAAGA AGAAGTGGCA AGCTGGAAAA 1001 CCGGCGACGT ATTGCTGTTG AACGGCAAAA TCCTCACCGG CCGCGATGCC 1051 GCACACAAAC GCCTCGTCGA TATGCTCAAC AAAGGCGAAG AATTGCCCGT 1101 CGATTTCACC AACCGCCTGA TTTACTACGT CGGCCCCGTC GATCCGGTCG 1151 GCGATGAAGT CGTCGGTCCG GCAGGTCCGA CCACAGCCAC CCGCATGGAC 1201 AAATTCACCC GCCAAATGCT CGAACAAACC GACCTCTTGG GCATGATCGG 1251 CAAATCCGAG CGCGGCGTGG CCACCTGCGA AGCCATCGCC GACAACAAAG 1301 CCGTGTACCT CATGGCAGTC GGCGGCGCGG CGTATCTCGT GGCAAAAGCC 1351 ATCAAATCTT CCAAAGTCTT GGCGTTCCCC GAATTGGGCA TGGAAGCCAT 1401 TTACGAATTT GAAGTCAAAG ACATGCCCGT AACCGTCGCC GTAGATAGCA 1451 AAGGCGAATC CATCCACGCC ACCGCCCCGC GCAAATGGCA GGCGAAAATC 1501 GGCATCATCC CCGTCGAATC TTGA This corresponds to the amino acid sequence <SEQ ID 220; ORF 050-l>: 050-l.pep 1 MTVIKQEDFI QSICDAFQFI SYYHPKDYID ALYKAWQKEE NPAAKDAMTQ 51 ILVNSRMCAE NNRPICQDTG IATVFLKVGM NVQWDADMSV EEMVNEGVRR 101 AYTWEGNTLR ASVLADPAGK RQNTKDNTPA VIHMSIVPGG KVEVTCAAKG 151 GGSENKSKLA MLNPSDNIVD WVLKTIPTMG AGWCPPGILG IGIGGTPEKA 201 VLMAKEΞLMS HIDIQELQEK AASGAELSTT EALRLELFEK VNALGIGAQG 251 LGGLTTVLDV KILDYPTHAA SKPIAMIPNC AATRHVEFEL DGΞGPVELTP 301 PRVEDWPDLT YSPDNGKRVD VDKLTKEEVA SWKTGDVLLL NGKILTGRDA 351 AHKRLVDMLN KGEELPVDFT NRLIYYVGPV DPVGDEVVGP AGPTTATRMD 401 KFTRQMLEQT DLLGMIGKSE RGVATCEAIA DNKAVYLMAV GGAAYLVAKA 451 IKSSKVLAFP ELGMEAIYEF EVKDMPVTVA VDSKGEΞIHA TAPRKWQAKI 501 GIIPVES* m050- l/g050-l 98.2% identity in 507 aa overlap 10 20 30 40 50 60 m050-l.pep MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAAKDAMTQILVNSRMCAE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I g050-l MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAAKDAMTQILVNΞRMCAE 10 20 30 40 50 60 70 80 90 100 110 120 m050-l.pep NNRPICQDTGIATVFLKVGMNVQWDADMSVEEMVNEGVRRAYTWEGNTLRASVLADPAGK I I I I I I I I I I I I I I I I I I I I :| I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I g050-l NNRPICQDTGIATVFLKVGMDVQWDADMΞVEKMVNEGVRRAYTWEGNTLRASVLADPAGK 70 80 90 100 110 120 130 140 150 160 170 180 m050-l.pep RQNTKDNTPAVIHMΞIVPGGKVEVTCAAKGGGΞENKSKLAMLNPSDNIVDWVLKTIPTMG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g050-l RQNTKDNTPAVIHMSIVPGGKVEVTCAAKGGGSENKSKLAMLNPSDNIVDWVLKTIPTMG 130 140 150 160 170 180 190 200 210 220 230 240 m050-l . pep AGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQEKAASGAELSTTEALRLELFEK I I I I II I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I g050-l AGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQEKAASGAELSTTEALRLELFEK 190 200 210 220 230 240 250 260 270 280 290 300 m050-l.pep VNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAMIPNCAATRHVEFELDGSGPVELTP I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g050-l VNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAMIPNCAATRHVEFELDGSGPVELTP 250 260 270 280 290 300 310 320 330 340 350 360 mO50-1. pep PRVEDWPDL SPDNGKRVDVDKLTKEEVASWKTGDVLLLNGKILTGRDAAHKRLVDMLN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : gO50-1 PRVEDXPDLTYSPDNGKRVDVDKLTKEEVASWKTGDVLLLNGKILTGRDAAHKRLVNMLD 310 320 330 340 350 360 370 380 390 400 410 420 m050-l.pep KGEELPVDFTNRLIYYVGPVDPVGDEVVGPAGPTTATRMDKFTRQMLEQTDLLGMIGKSE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I g050-l KGEELPVDFTNRLIYYVGPVDPVGDEVVGPAGPTTATRMDKFTRQMLKQTGLLGMIGKSE 370 380 390 400 410 420 430 440 450 460 470 480 m050-l.pep RGVATCEAIADNKAVYLMAVGGAAYLVAKAIKSΞKVLAFPELGMEAIYEFEVKDMPVTVA I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I :| I I I I I I I I I I I I g050-l RGAATCEAIADNKAVYLMAVGGAAYLVAKAIKSSKVLAFPELGMEAVYEFEVKDMPVTVA 430 440 450 460 470 480 490 500 m050-l .pep VDSKGESIHATAPRKWQAKIGIIPVESX I I I I I I I I I I I I I I I I I I I I I I I I I I I I g050-l VDSKGESIHATAPRKWQAKIGIIPVESX 490 500 The following partial DNA sequence was identified in N meningitidis <SEQ ID 221>: a050-l.seq 1 ATGACCGTCA TCAAACAGGA AGACTTTATC CAAAGCATTT GCGATGCCTT 51 CCAATTCATC AGCTACTACC ATCCCAAAGA CTACATCGAC GCGCTTTATA 101 AGGCGTGGCA GAAGGAAGAA AACCCCGCCG CCAAAGACGC GATGACGCAG 151 ATTTTGGTCA ACAGCCGCAT GTGTGCCGAA AACAACCGCC CCATCTGCCA 201 AGATACCGGT ATCGCGACCG TGTTTTTGAA AGTCGGTATG GATGTGCAAT 251 GGGATGCAGA CATGAGCGTC GAAGAGATGG TTAACGAAGG CGTGCGCCGC 301 GCCTACACTT GGGAAGGCAA TACGCTGCGC GCTTCCGTTC TCGCCGACCC 351 CGCCGGCAAA CGCCAAAATA CCAAAGACAA CACGCCCGCC GTCATCCATA 401 TGAGCATCGT GCCGGGCGAC AAAGTCGAAG TAACCTGCGC GGCAAAAGGC 451 GGCGGTTCTG AAAACAAATC CAAACTCGCC ATGCTCAACC CTTCCGACAA 501 CATCGTCGAT TGGGTATTGA AAACCATTCC GACCATGGGC GCGGGCTGGT 551 GTCCTCCCGG CATCTTGGGC ATCGGCATCG GCGGTACGCC CGAAAAAGCC 601 GTGTTGATGG CGAAAGAATC CCTGATGAGC CACATCGACA TCCAAGAATT 651 GCAGGAAAAA GCCGCGTCCG GCGCGGAATT GTCCACCACC GAAGCCCTGC 701 GCCTCGAACT CTTTGAAAAA GTCAACGCGC TAGGCATCGG CGCGCAAGGC 751 TTGGGCGGTC TGACCACCGT GTTGGACGTG AAAATCCTCG ATTACCCGAC 801 CCACGCCGCC TCCAAACCGA TTGCCATGAT TCCGAACTGC GCCGCCACCC 851 GCCACGTCGA ATTTGAATTG GACGGCTCAG GCCCTGTCGA ACTCACGCCG 901 CCGCGCGTCG AAGACTGGCC CGATTTGACT TACAGCCCCG ACAACGGCAA 951 ACGCGTCGAT GTCGACAAGC TGACCAAAGA AGAAGTGGCA AGCTGGAAAA 1001 CCGGCGACGT ATTGCTGTTG AACGGCAAAA TCCTCACCGG CCGCGATGCC 1051 GCACACAAAC GCCTCGTCGA TATGCTCGAC AAAGGCGAAG AATTGCCCGT 1101 CGATTTCACC AACCGCCTGA TTTACTACGT CGGCCCCGTC GATCCGGTCG 1151 GCGACGAAAT CGTCGGCCCA GCAGGTCCGA CCACCGCCAC CCGCATGGAC 1201 AAATTCACCC GCCAAATGCT CGAACAAACC GACCTCTTGG GCATGATCGG 1251 CAAATCCGAG CGCGGCGCGG CCACCTGCGA AGCCATCGCC GACAACAAAG 1301 CCGTGTACCT CATGGCAGTC GGCGGCGCGG CGTATCTCGT GGCAAAAGCC 1351 ATCAAATCTT CCAAAGTCTT GGCGTTCCCC GAATTGGGCA TGGAAGCCAT 1401 TTACGAATTT GAAGTCAAAG ACATGCCCGT AACCGTCGCC GTAGACAGCA 1451 AAGGCGAATC CATCCACGCC ACCGCCCCGC CCCAATGGCA GGCGAAAATC 1501 GGCATCATCC CCGTCAAATC TTGA This corresponds to the amino acid sequence <SEQ ID 222; ORF 050-l.a>: a050-l.pep 1 MTVIKQEDFI QSICDAFQFI SYYHPKDYID ALYKAWQKEE ΝPAAKDAMTQ 51 ILVΝSRMCAE ΝΝRPICQDTG IATVFLKVGM DVQWDADMSV EEMVΝEGVRR 101 AYTWEGΝTLR ASVLADPAGK RQΝTKDΝTPA VIHMSIVPGD KVEVTCAAKG 151 GGSEΝKSKLA MLΝPSDΝIVD WVLKTIPTMG AGWCPPGILG IGIGGTPEKA 201 VLMAKESLMS HIDIQELQEK AASGAELSTT EALRLELFEK VΝALGIGAQG 251 LGGLTTVLDV KILDYPTHAA SKPIAMIPΝC AATRHVEFEL DGSGPVELTP 301 PRVEDWPDLT YSPDΝGKRVD VDKLTKEEVA ΞWKTGDVLLL ΝGKILTGRDA 351 AHKRLVDMLD KGEELPVDFT ΝRLIYYVGPV DPVGDEIVGP AGPTTATRMD 401 KFTRQMLEQT DLLGMIGKSE RGAATCEAIA DΝKAVYLMAV GGAAYLVAKA 451 IKSSKVLAFP ELGMEAIYEF EVKDMPVTVA VDSKGEΞIHA TAPPQWQAKI 501 GIIPVKS* a050-l/ 050-l 98.4% identity in 507 aa overlap 10 20 30 40 50 60 a050-l.pep MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAAKDAMTQILVNSRMCAE I 1 I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I mO50-1 MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAAKDAMTQILVNSRMCAE 10 20 30 40 50 60 70 80 90 100 110 120 aO50-1. pep NNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAYTWEGNTLRASVLADPAGK I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m050-l NNRPICQDTGIATVFLKVGMNVQWDADMSVEEMVNEGVRRAYTWEGNTLRASVLADPAGK 70 80 90 100 110 120 130 140 150 160 170 180 a050-l.pep RQNTKDNTPAVIHMSIVPGDKVEVTCAAKGGGSENKSKLAMLNPSDNIVDWVLKTIPTMG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m050-l RQNTKDNTPAVIHMSIVPGGKVEVTCAAKGGGSENKSKLAMLNPSDNIVDWVLKTIPTMG 130 140 150 160 170 180 190 200 210 220 230 240 aO50-1. pep AGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQEKAASGAELSTTEALRLELFEK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m050-l AGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQEKAASGAELSTTEALRLELFEK 190 200 210 220 230 240 250 260 270 280 290 300 aO50-1. ep VNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAMIPNCAATRHVEFELDGSGPVELTP I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m050-l VNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAMIPNCAATRHVEFELDGΞGPVELTP 250 260 270 280 290 300 310 320 330 340 350 360 a050-l.pep PRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKTGDVLLLNGKILTGRDAAHKRLVDMLD I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : m050-l PRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKTGDVLLLNGKILTGRDAAHKRLVDMLN 310 320 330 340 350 360 370 380 390 400 410 420 a050-l.pep KGEELPVDFTNRLIYYVGPVDPVGDEIVGPAGPTTATRMDKFTRQMLEQTDLLGMIGKSE I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m050-l KGEELPVDFTNRLIYYVGPVDPVGDEVVGPAGPTTATRMDKFTRQMLEQTDLLGMIGKSE 370 380 390 400 410 420 430 440 450 460 470 480 a050-l.pep RGAATCEAIADNKAVYLMAVGGAAYLVAKAIKSSKVLAFPELGMEAIYEFEVKDMPVTVA I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I mO50-1 RGVATCEAIADNKAVYLMAVGGAAYLVAKAIKSSKVLAFPELGMEAIYEFEVKDMPVTVA 430 440 450 460 470 480 490 500 a050-l .pep VDSKGESIHATAPPQWQAKIGIIPVKSX I I I I I I I I I I I I I : I I I I I I I I I I :l I m050-l VDSKGESIHATAPRKWQAKIGIIPVESX 490 500 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 223>: g052.seq 1 ATGGCTTTGG TGGCGGAGGA AACGGAAATA TCCGCGCCGT GTTTCAAAGG 51 CTGCGAGCCG ACGGGCGACA GCAGGCTGTT GTCCACCACC AAGAGCGCGC 101 CGATGCCGTG CGCCAATTCC GCCAAGGCTT CCAAGTCGGC CACTTCGCCC 151 AAGGGGTTGG ACGGCGTTTC CAAAAACAGC AGTTTGGTGT TGGCTTTGAC 201 GGCGGCTTTC CATTCATTTA TATCAGTCGG CGACACGCGG CTCACTCCGA 251 TGCCGAATTT GGTAACGATG TTATTGATAA AGCCGACGGT CGTGCCGAAC 301 AGGCTGCGGC TGGAAACCAC ATGGTCGCCC GCCTGCAGGA AGGTGAAAAA 351 CGCCGCCTGA This corresponds to the amino acid sequence <SEQ ID 224; ORF 052.ng>: g052 . pep 1 MALVAEETEI SAPCFKGCEP TGDSRLLSTT KSAPMPCANS AKASKSATSP 51 KGLDGVSKNS SLVLALTAAF HSFISVGDTR LTPMPNLVTM LLIKPTWPN 101 RLRLETTWSP ACRKVKNAA* The following partial DNA sequence was identified in N meningitidis <SEQ ID 225>: m052. seq 1 ATGGCTTTGG TGGCGGAGGA AACGGAAATA TCCGCGCCGT GTTTCAAAGG 51 CTGCGAGCCG ACGGGCGACA GCAGGCTGTT GTCCACCACC AAGAGCGCGC 101 CGATGCCGTG CGCCAATTCC GCCAAGGCTT CCAAGTCGGC CACTTCGCCC 151 AAGGGGTTGG ACGGCGTTTC CAAAAACAGC AGTTTGGTGT TGGCTTTGAC 201 GGCGGCTTTC CATTCATTTA TATCAGTCGG CGACACGCGG CTCACTCCGA 251 TGCCGAATTT GGTAACGATG TTATTGATAA AGCCGACGGT CGTGCCGAAC 301 AGGCTGCGGC TGGAAACCAC ATGGTCGCCC GCCTGCAGGA AGGTGAAAAA 351 CGCCGCCTGA This corresponds to the amino acid sequence <SEQ ID 226; ORF 052>: m052.pep 1 MALVAEETEI SAPCFKGCEP TGDSRLLSTT KSAPMPCANS AKASKSATSP 51 KGLDGVSKNS SLVLALTAAF HSFISVGDTR LTPMPNLVTM LLIKPTWPN 101 RLRLETTWSP ACRKVKNAA* The following partial DNA sequence was identified in N meningitidis <SEQ ID 227>: a052. seq 1 ATGGCTTTGG TCGCGGAGGA AACGGAAATA TCCGCGCCGT GTTTCAAAGG 51 CTGAGAGCCG ACAGGCGACA GCAGGCTGTT GTCCACCACC AAGAGCGCGC 101 CGATGCCGTG CGCCAATTCC GCCAAGGCTT CCAAGTCGGC CACTTCTCCC 151 AAGGGATTGG ACGGCGTTTC CAAAAACAGC AGTTTGGTGT TGGCTTTGAC 201 GGCGGCTTTC CATTCGTTTA TATCAGTCGG CGACACGTGA CTCACTTCGA 251 TGCCGAATTT GGTAACGATG TTATTGATAA AGCCGACGGT CGTGCCGAAC 301 AGGCTGCGGC TGGAAATCAC ATGGTCGCCC GCCTGCAAAA AGGTGAAAAA 351 CGCCGCCTGA This corresponds to the amino acid sequence <SEQ ID 228; ORF 052. a>: a052.pep 1 MALVAEETEI SAPCFKG*EP TGDSRLLSTT KSAPMPCANS AKASKSATSP 51 KGLDGVSKNS SLVLALTAAF HSFISVGDT* LTSMPNLVTM LLIKPTWPN 101 RLRLEITWSP ACKKVKNAA* m052/a052 95.8%o identity over a 119 aa overlap 10 20 30 40 50 60 m052. pep MALVAEETEISAPCFKGCEPTGDSRLLSTTKSAPMPCANSAKASKSATSPKGLDGVSKNS II I I I II II I II I II II II I I II I II I I II I II I II I II I I II I II II II I II I I I I I I a052 MALVAEETEISAPCFKGXEPTGDSRLLSTTKSAPMPCANSAKASKSATSPKGLDGVSKNS 10 20 30 40 50 60 70 80 90 100 110 120 m052. pep SLVLALTAAFHSFISVGDTRLTPMPNLVTMLLIKPTWPNRLRLETTWSPACRKVKNAAX II I I I II II I II I II II I I II I II II I I I II I I II I II II II I I II II I : II I I II I a052 SLVLALTAAFHSFISVGDTXLTSMPNLVTMLLIKPTVVPNRLRLEITWSPACKKVKNAAX 70 80 90 100 110 120 Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 052 shows 95.8% identity over a 119 aa overlap with a predicted ORF (ORF 052.ng) from N gonorrhoeae: m052/g052 10 20 30 40 50 60 m052. pep MALVAEETEISAPCFKGCEPTGDSRLLSTTKSAPMPCAΝSAKASKSATSPKGLDGVSKΝS g052 MALVAEETEISAPCFKGCEPTGDSRLLSTTKSAPMPCANSAKASKSATSPKGLDGVSKNS 10 20 30 40 50 60 70 80 90 100 110 120 m052.pep SLVLALTAAFHSFISVGDTWLTSMPNLATMLLIKPTWPNRLRLEITWSPACKKVKNAAX M M M M M M I M M M I I I I I I = 1 1 I I I I I 1 1 I I 1 1 1 I I I M M M M M M M g052 SLVLALTAAFHSFISVGDTRLTPMPNLVTMLLIKPTWPNRLRLETTWSPACRKVKNAAX 70 80 90 100 110 120 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 229>: g073.seq 1 ATGTGTATGC CATACGCAAT AAGGGTTTCA GACGGCATCT GCCGCATTTT 51 TCCGCCGATG CCGTCTGAAA CACGCAATCA GCGCGCGAGT GCCTGTTTCA 101 AATCGTCAAT CAAATCGCCA ACATATTCCA AACCGACCGA CAGGCGCACC 151 AGTCCGGGGC GGatacCGGC GGCGAGTTTT TCTTCGGGCT GCATCCTGCC 201 GTGCGTGGTT GTCCACGGAT TGGTGATGGT CGAGCGCACG TCGCCGAGGT 251 TGGCGGTACG GGAAAAGAGT TCCACGACTT TCCACGCGGC TGCTTGGTCG 301 GCGACTTCAA AACCGATGAC GATGCCGCCG CCGTTTTGCT GTTTGCGGAT 351 AAGCTCCGCC TGCGGATGGT CGGGCAATCC GGTGTAG This corresponds to the amino acid sequence <SEQ ID 230; ORF 073.ng>: g073.pep 1 MCMPYAIRVS DGICRIFPPM PSETRΝQRAS ACFKSSIKSP TYSKPTDRRT 51 SPGRIPAASF SSGCILPCW VHGLVMVERT SPRLAVREKS STTFHAAAWS 101 ATSKPMTMPP PFCCLRISSA CGWSGΝPV* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 231>: m073.seq 1 ATGTGTATGC CATATAAGAT AAGGGTTTCA GACGGCATCT GCTGTCCAAT 51 GCCGTCTGAA ACACGCAATC AGCGTGCGAG TGCCTGTTTC AAATCGTCAA 101 TCAAATCGCC AACATATTCC AAACCGACCG ACAGGCGCAC CAATCCGGGG 151 CGGATGTTGG CGGCGAGTTT TTCTTCGGGC TGCATCCTGC CGTGCGTGGT 201 TGTCCACGGG TGGGTAATGG TCGAGCGCAC GTCACCGAGG TTGGCGGTGC 251 GGGAAAAGAG TTCCACGCCG TCCACAACTT TCCACGCCGC TTCTTGATCG 301 GCAACTTCAA AGCCGATGAC GATGCCGCCG CCGTTTTGCT GTTTGCGGAT 351 AAGCGCCGCC TGAGGATGGT CGGACAATCC GGTGTAG This corresponds to the amino acid sequence <SEQ ID 232; ORF 073>: m073.pep 1 MCMPYKIRVS DGICCPMPSE TRΝQRASACF KSSIKSPTYS KPTDRRTΝPG 51 RMLAASFSSG CILPCVWHG WVMVERTSPR LAVREKSSTP STTFHAASXS 101 ATSKPMTMPP PFCCLRISAA XGWSDΝPV* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 233>: a073.seq 1 ACGTGTATGT CATATAAGAT AAGGGTTTCA GACGGCATTT GCGGTGTTTT 51 TCCGCCGATG CCGTCTGAA. CACGCAATCA GCGCGCGAGT GCCTGTTTCA 101 AATCGTCAAT CAAATCGCCA ACATATTCCA AACCGACCGA CAGGCGCACC 151 AATCCGGGGC GGATGTTGGC GGCGAGTTTT TCTTCGGGCT GCATCCTGCC 201 GTGCGTGGTT GTCCACGGAT GGGTAATGGT CGAGCGCACG TCGCCGAGGT 251 TGGCGGTACG GGAGAAAAGT TCGACGCCGT CCACGACTTT CCACGCGGCT 301 GCTTGGTCGG CGACTTCAAA GCCGATGACG ATGCCGCCGC CGTTTTGCTG 351 TTTGCGGATA AGCTCCGCCT GAGGATGGTC GGGTAATCCG GTGTAA This corresponds to the amino acid sequence <SEQ ID 234; ORF 073. a>: a073.pep 1 TCMSYKIRVS DGICGVFPPM PSEXRΝQRAS ACFKSSIKSP TYSKPTDRRT 51 ΝPGRMLAASF SSGCILPCW VHGWVMVERT SPRLAVREKS STPSTTFHAA 101 AWSATSKPMT MPPPFCCLRI SSA*GWSGΝP V* m073/a073 92.3%o identity over a 130 aa overlap 10 20 30 40 50 m073.pep MCMPYKIRVSDGICC PMPSETRNQRASACFKSSIKSPTYSKPTDRRTNPGRMLAASF II II I I II II II II II I : II II II II II II I I I I II II II I II II II II II II I a073 TCMSYKIRVSDGICGVFPPMPSEXRNQRASACFKSSIKSPTYSKPTDRRTNPGRMLAASF 10 20 30 40 50 60 60 70 80 90 100 110 m073.pep SSGCILPCVWHGWVMVERTSPRLAVREKSSTPSTTFHAASXSATSKPMTMPPPFCCLRI I I II II II II II I I I II II II II I II II II I II I II II I I : II II II II II II II II II a073 SSGCILPCWVHGWVMVERTSPRLAVREKSSTPSTTFHAAAWSATSKPMTMPPPFCCLRI 70 80 90 100 110 120 120 129 m073.pep SAAXGWSDNPVX I : I II II I II I a073 SSAXGWSGNPVX 130 Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 073 shows 87.0% identity over a 131 aa overlap with a predicted ORF (ORF 073.ng) from N gonorrhoeae: m073/g073 10 20 30 40 50 m073.pep MCMPYKIRVSDGICC PMPSETRΝQRASACFKSSIKSPTYSKPTDRRTΝPGRMLAASF M i l l 1 1 1 1 1 1 1 1 M M M M M M M M M M M M M M M M M I h M M g073 MCMPYAIRVSDGICRIFPPMPSETRΝQRASACFKSSIKSPTYSKPTDRRTSPGRIPAASF 10 20 30 40 50 60 60 70 80 90 100 110 m073.pep SSGCILPCVWHGWVMVERTSPRLAVREKSSTPSTTFHAASXSATSKPMTMPPPFCCLRI M M I M M M M M M M M M M M M M 9 1 1 1 1 = I I I 1 1 I I I I I 1 1 I I 1 1 I I g073 SSGCILPCVWHGLVMVERTSPRLAVREKSST TFHAAAWSATSKPMTMPPPFCCLRI 70 80 90 100 110 120 129 m073.pep SAAXGWSDNPVX IM III MM g073 SSACGWSGNPVX 120 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 235>: g075.seq 1 ATGCCGCCTT ACTTCATCAC CCTCTTAACG ATGGAAAATA CAAAAAGCGC 51 GGCGAAAACG CCCACTACAA TCCAACCGGC TTCCATACCG TCCGCTTTTG 101 CGGCTTCCAA AGCGTTTTTT GCCGTTTCGG GCAACGCTGC GTTTGCCTGT 151 GCCGCCAAAG CCAGCGGGGC GGCTGTTACA ACAGCCAGTT TTGCGCCGTA 201 TTTACGGCAG GTGTTAATAA ATTTCATGAT ATTTTCCTTT ACGAAATTTT 251 TAAAAAAATG TGTTTGCGGG CTTTGTGAAG GTTTTAGAGA CCGCCTGCCG 301 GGCCTCTTAA ACTTAATCTT CTTTTTCGTA GAATCCGAAA ATTACAAATT 351 CCCCGCCTAT CTCTTCCAAT GCCGAGCTAA AAGCGTCTTC ATAGCTGTCA 401 TATTTACCGG CTGA This corresponds to the amino acid sequence <SEQ ID 236; ORF 075.ng>: g075.pep 1 MPPYFITLLT MEΝTKSAAKT PTTIQPASIP SAFAASKAFF AVSGΝAAFAC 51 AAKASGAAVT TASFAPYLRQ VLIΝFMIFSF TKFLKKCVCG LCEGFRDRLP 101 GLLΝLIFFFV ESEΝYKFPAY LFQCRAKSVF IAVIFTG* The following partial DNA sequence was identified in N meningitidis <SEQ ID 237>: m075.seq 1 ATGCCGTCTT ACTTCATCAC TCTCTTAACG ATGGAAAATA CAAAAAGCGC 51 GGCGAAAATG CCCACTACAA TCCAACCGGC TTCCATACCG TCCGCTTTTG 101 CGGCTTCCAA AGCGTTTTTT GCCGTATCGG GCAACGTTGC ATTTGCATGT 151 GCGGCCAAAG CCAGGGGAGC AGCTGTTACA ACAGCCAGTT TTGCGCCGTA 201 TTTACGGCAG GTGTTAATAA ATTTCATGAT ATTTTCCTTC AAAAAGTGTT 251 TGGCGGTAAT GGATGGAGCG TTTTTCAGAC GACCGCCGAA CATCCGAAAA 301 TCAGTCTTTC AAAAATCCGA ATACGACAAA TTCGTATTGG TTGCCGATTT 351 CTTCCAAACC TGCGTTAATC GCTTCTTCGA AGTCGTAGAA ATAATCGGCA 401 TTGGTGATTA A This corresponds to the amino acid sequence <SEQ ID 238; ORF 075>: m075.pep 1 MPSYFITLLT MEΝTKSAAKM PTTIQPASIP SAFAASKAFF AVSGΝVAFAC 51 AAKARGAAVT TASFAPYLRQ VLIΝFMIFSF KKCLAVMDGA FFRRPPΝIRK 101 SVFQKSEYDK FVLVADFFQT CVΝRFFEWE IIGIGD* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 075 shows 65.7% identity over a 137 aa overlap with a predicted ORF (ORF 075.ng) from N gonorrhoeae: m075/g075 10 20 30 40 50 60 m075.pep MPSYFITLLTMEΝTKSAAKMPTTIQPASIPSAFAASKAFFAVSGΝVAFACAAKARGAAVT I I M M M M M M M M 1 1 1 I I 1 1 1 I I I I I I I I I I 1 1 I I I I I M 1 1 I I I I I M i l l g075 MPPYFITLLTMEΝTKSAAKTPTTIQPASIPSAFAASKAFFAVSGΝAAFACAAKASGAAVT 10 20 30 40 50 60 70 80 90 100 110 m075. pep TASFAPYLRQVLIΝFMIFSF KKCLAVMDGAFFRRPPΝIRKSVFQKSEYDKFVLVAD MMMMMMMMMM MM ■ ■ M I h = = M I = = = = I g075 TASFAPYLRQVLIΝFMIFSFTKFLKKCVCGLCEGFRDRLPGLLΝLIFFFVESEΝYKFPAY 70 80 90 100 110 120 120 130 m075.pep FFQTCVΝRFFEWEIIGIGDX :|| :: | :| : | g075 LFQCRAKSVFIAVIFTGX 130 The following partial DΝA sequence was identified inN meningitidis <SEQ ID 239>: a075.seq 1 ATGCCGTCTT ACTTCATCAC TCTCTTAACG ATGGAAAAGA CAAAAAGCGC 51 GGCGAAAACG CCCACTACAA TCCAACCGGC TTCCATACCG TCCGCTTTTG 101 CGGCTTCCAA AGCGTTTTTT GCTGTATCGG GCAACGTTGC ATTTGCATGT 151 GCGGCCAAAG CCAGGGGAGC AGCTGTTACA ACAGCCAGTT TTGCGCCGTA 201 TTTACGGCAG GTGTTAATAA ATTTCATGAT ATTTTCCTTC AAAAAGTGTT 251 TGGCGGTAAT GGATGGAGCG TTTTTCAGAC GACCGCCGAA CATCCGAAAA 301 TCAGTCTTTC AAAAATCCGA ATACGACAAA TTCGTATTGG TTGCCGATTT 351 CTTCCAAACC TGCGTTAATC GCTTCTTCGA AGTCGTAGAA ATAATCGGCA 401 TTGGTGATTA A This corresponds to the amino acid sequence <SEQ ID 240; ORF 075. a>: a075.pep 1 MPSYFITLLT MEKTKSAAKT PTTIQPASIP SAFAASKAFF AVSGΝVAFAC 51 AAKARGAAVT TASFAPYLRQ VLIΝFMIFSF KKCLAVMDGA FFRRPPΝIRK 101 SVFQKSEYDK FVLVADFFQT CVΝRFFEWE IIGIGD* m075 a075 98.5%o identity over a 136 aa overlap 10 20 30 40 50 60 m075. pep MPSYFITLLTMENTKSAAKMPTTIQPASIPSAFAASKAFFAVSGNVAFACAAKARGAAVT II I II I II I II I : II II II I II I II I I II II II II II II II I II II I II I I II I I II I I a075 MPSYFITLLTMEKTKSAAKTPTTIQPASIPSAFAASKAFFAVSGNVAFACAAKARGAAVT 10 20 30 40 50 60 70 80 90 100 110 120 m075. pep TASFAPYLRQVLINFMIFSFKKCLAVMDGAFFRRPPNIRKSVFQKSEYDKFVLVADFFQT I I I I I II I II II II II II II II II I II II II I I II II II II II I II II I I II II I I II II a075 TASFAPYLRQVLINFMIFSFKKCLAVMDGAFFRRPPNIRKSVFQKSEYDKFVLVADFFQT 70 80 90 100 110 120 130 m075.pep CVNRFFEWEIIGIGDX I II I II I II II I I II II a075 CVNRFFEWEIIGIGDX 130 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 241>: g080. seq 1 ATGTGGGATA ATGCCGAAGC GATGGAACGG CTGACGCGCT GGCTGCTTGT 51 CATGATGGCG ATGCTGCTTG CTGCGTCCGG GCTGGTTTGG TTTTACAATT 101 CGAATCATCT GCCCGTCAAG CAGGTGTCGC TGAAGGGCAA CCTGGTTTAT 151 TCCGATAAGA AGGCATTGGG CAGTTTGGCG AAAGAATACA TCCATGGGAA 201 TATTTTGAGG ACGGACATCA ATGGCGCACA GGAAGCCTAC CGCCGGTATC 251 CGTGGATTGC GTCGGTCATG GTGCGCCGCC GTTTTCCCGA TACGGTTGAG 301 GTCGTCCTGA CCGAGCGCAA GCCGGTTGCA CGTTGGGGCG ACCATGCCTT 351 GGTGGACGGC GAAGGCAATG TTTTTGAAGC CCGCTTGGAC AGACCCGGAA 401 TGCCGGTATT CAGAGGCGCG GAAGGAACGT CTGCCGAAAT GCTCCGCCGT 451 TATGACGAAT TTTCGACTGT TTTGGCAAAA CAGGGTTTGG GCATCAAAGA 501 GATGACCTAT ACGGCACGTT CGGCGTGGAA TGTCGTTTTG GACAACGGCA 551 TCACCGTCAG GCTCGGACGG GAAAAcgaGA TGAAACGCCT CCgGCTTTTT 601 ACcgAAGCGT GGCAGCATCT gttgcGTAAG AATAAAAATC GGTTATCCTA 651 TGTGGATATG Aggtataagg acggatttTC agtcccccat gctCCCGACG 701 GTTTACCCGA AAAAGAATcc gAAGAATatt gggaacaggt ttgggacata 751 ttacggcctg gcgtcggaaa cggttcgacg caaatttcaa tcagttatAA 801 GGGCAGacga acaatggaac AGcagtaa This corresponds to the amino acid sequence <SEQ ID 242; ORF 080.ng>: g080.pep 1 MWDΝAEAMER LTRWLLVMMA MLLAASGLVW FYΝSΝHLPVK QVSLKGΝLVY 51 SDKKALGSLA KEYIHGΝILR TDIΝGAQEAY RRYPWIASVM VRRRFPDTVE 101 WLTERKPVA RWGDHALVDG EGΝVFEARLD RPGMPVFRGA EGTSAEMLRR 151 YDEFSTVLAK QGLGIKEMTY TARSAWΝWL DΝGITVRLGR EΝEMKRLRLF 201 TEAWQHLLRK ΝKΝRLSYVDM RYKDGFSVPH APDGLPEKES EEYWEQVWDI 251 LRPGVGΝGST QISISYKGRR TMEQQ* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 243>: m080. seq 1 ATGTGGGATA ATGCCGAAGC GATGGAACGG CTGACGCGCT GGCTGCTTGT 51 CATGATGGCG ATGCTGCTTG CTGCGTCCGG GCTGGTTTGG TTTTACAATT 101 CGAATCATCT GCCCGTCAAG CAGGTGTCGC TGAAGGGCAA CCTGGTTTAT 151 TCCGATAAGA AGACATTGGG CAGTTTGGCG AAAGAATACA TCCATGGGAA 201 TATTTTGAGG ACGGACATCA ATGGCGCACA GGAGGCCTAC CGCCGGTATC 251 CGTGGATTGC GTCGGTCATG GTGCGCCGCC GTTTTCCCGA CACGGTTGAG 301 GTCGTCCTGA CCGAGCGCAA GCCGGTCGCG CGTTGGGGCG ACCATGCCTT 351 GGTGGACGGC GAAGGCAATG TTTTTGAAGC CCGCTTGGAC AGACCCGGAA 401 TGCCGGTATT CAGAGGCGCG GAAGGAACGT CTGCCGAAAT GCTCCGCCGT 451 TATGACGAAT TTTCGACTGT TTTGGCAAAA CAGGGTTTGG GCATCAAAGA 501 GATGACCTAT ACGGCACGTT CGGCGTGGAT TGTCGTTTTG GACAACGGCA 551 TCACCGTCAG GCTCGGACGG GAAAACGAGA TGAAACGCCT CCGGCTTTTT 601 ACCGAAGCGT GGCAGCATCT GTTGCGTAAA AATAAAAATC GGTTATCCTA 651 TGTGGATATG AGGTATAAGG ACGGATTTTC AGTCCGCTAT GCTTCCGACG 701 GTTTACCCGA AAAAGAATCC GAAGAATAG This corresponds to the amino acid sequence <SEQ ID 2441; ORF 080>: m080.pep 1 MWDNAEAMER LTRWLLVMMA MLLAASGLVW FYNSNHLPVK QVSLKGNLVY 51 SDKKTLGSLA KEYIHGNILR TDINGAQEAY RRYPWIASVM VRRRFPDTVE 101 WLTERKPVA RWGDHALVDG EGNVFEARLD RPGMPVFRGA EGTSAEMLRR 151 YDEFSTVLAK QGLGIKEMTY TARSAWIWL DNGITVRLGR ENEMKRLRLF 201 TEAWQHLLRK NKNRLSYVDM RYKDGFSVRY ASDGLPEKES EE* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 080 shows 97.9% identity over a 242 aa overlap with a predicted ORF (ORF 080.ng) from N gonorrhoeae: m080/g080 10 20 30 40 50 60 mO80. pep MWDΝAEAMERLTRWLLVMMAMLLAASGLVWFYΝSΝHLPVKQVSLKGΝLVYSDKKTLGSLA MMMMMMMMIMMMMMMMMMMMMMMMMIMMMMM 080 MWDΝAEAMERLTRWLLVMMAMLLAASGLVWFYΝSΝHLPVKQVSLKGΝLVYSDKKALGSLA 10 20 30 40 50 60 70 80 90 100 110 120 mO 80. pep KEYIHGΝILRTDIΝGAQEAYRRYPWIASVMVRRRFPDTVEWLTERKPVARWGDHALVDG MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM 080 KEYIHGΝILRTDIΝGAQEAYRRYPWIASVMVRRRFPDTVEWLTERKPVARWGDHALVDG 70 80 90 100 110 120 130 140 150 160 170 180 mO80. pep EGΝVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAKQGLGIKEMTYTARSAWIWL MMMMMMMMMMMMMMMMMMMMMMMMMMMM Ml 080 EGΝVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAKQGLGIKEMTYTARSAWΝWL 130 140 150 160 170 180 190 200 210 220 230 240 mO80. pep DΝGITVRLGREΝEMKRLRLFTEAWQHLLRKΝKΝRLSYVDMRYKDGFSVRYASDGLPEKES MMMMMMIMMMMMMMMMMMMMIMMMM M 080 DΝGITVRLGREΝEMKRLRLFTEAWQHLLRKΝKΝRLSYVDMRYKDGFSVPHAPDGLPEKES 190 200 210 220 230 240 m080.pep EEX II 080 EEYWEQVWDILRPGVGΝGSTQISISYKGRRTMEQQX 250 260 270 The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 245>: aOΘO.seq 1 ATGTGGGATA ATGCCGAAGC GATGGAACGG CTGACGCGCT GGCTGCTTGT 51 CATGATGGCG ATGCTGCTTG CTGCGTCCGG GCTGGTTTGG TTTTACAATT 101 CGAATCATCT GCCCGTCAAG CAGGTGTCGC TGAAGGGCAA CCTAGTTTAT 151 TCCGATAAGA AAGCATTGGG CAGTTTGGCG AAAGAATACA TCCATGGGAA 201 TATTTTGAGG ACGGACATCA ATGGCGCACA GGAGGCCTAC CGCCGGTATC 251 CGTGGATTGC GTCGGTCATG GTGCGCCGCC GTTTTCCCGA CACGGTTGAG 301 GTCGTCCTGA CCGAGCGCAA GCCGGTCGCG CGTTGGGGCG ACCATGCCTT 351 GGTGGACGGC GAAGGCAATG TTTTTGAAGC CCGTTTGGAC AGACCCGGAA 401 TGCCGGTATT CAGAGGCGCG GAAGGAACGT CTGCCGAAAT GCTCCGCCGT 451 TATGACGAAT TTTCGACTGT TTTGGCAAAA CAGGGTTTGG GCATCAAAGA 501 GATGACCTAT ACGGCACGTT CGGCGTGGAT TGTCGTTTTG GACAACGGCA 551 TCACCGTCAG GCTCGGACGG GAAAACGAGA TGAAACGCCT CCGGCTTTTT 601 ACCGAAGCGT GGCAACATCT GTTGCGTAAA AATAAAAATC GGTTATCCTA 651 TGTGGATATG AGGTATAAGG ACGGATTTTC AGTCCGCTAT GCTCCCGACG 701 GTTTACCCGA AAAAGAATCC GAAGAATAG This corresponds to the amino acid sequence <SEQ ID 246; ORF 080. a>: aOδO.pep 1 MWDNAEAMER LTRWLLVMMA MLLAASGLVW FYNSNHLPVK QVSLKGNLVY 51 SDKKALGSLA KEYIHGNILR TDINGAQEAY RRYPWIASVM VRRRFPDTVE 101 WLTERKPVA RWGDHALVDG EGNVFEARLD RPGMPVFRGA EGTSAEMLRR 151 YDEFSTVLAK QGLGIKEMTY TARSAWIWL DNGITVRLGR ENEMKRLRLF 201 TEAWQHLLRK NKNRLSYVDM RYKDGFSVRY APDGLPEKES EE* m080/a080 99.2%> identity over a 242 aa overlap 10 20 30 40 50 60 m080. pep MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSLKGNLVYSDKKTLGSLA I II I II II II II I II II II II II I II II II II II II II II II II II I II II I II : II I II a080 MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSLKGNLVYSDKKALGSLA 10 20 30 40 50 60 70 80 90 100 110 120 m080. pep KEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEWLTERKPVARWGDHALVDG II I II I II I II I II II II II II I II II II II II I I I I II I II I II II II I II I II I II I I a080 KEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEWLTERKPVARWGDHALVDG 70 80 90 100 110 120 130 140 150 160 170 180 m080. pep EGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAKQGLGIKEMTYTARSAWIWL I I I I II I II I I I II II II II II I I I II II I I II II II I II II II II I II II I I I I II I II a080 EGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAKQGLGIKEMTYTARSAWIWL 130 140 150 160 170 180 190 200 210 220 230 240 m080.pep DNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYVDMRYKDGFSVRYASDGLPEKES I II I II I II I II II II II II I II II II II II II II I II I II I II I I I II II II II I II I a080 DNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYVDMRYKDGFSVRYAPDGLPEKES 190 200 210 220 230 240 m080.pep EEX I I I a080 EEX The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 247>: gOδl.seq 1 ATGAAACCAC TGGACCTAAA TTTCATCTGC CAAGCCCTCA AGCTTCCGAT 51 GCCGTCTGAA AACAAACCCG TGTCGCGCAT CGTAACCGAC AGCCGCGATA 101 TTCGGGAAGG CGATGTGTTT TTCGCATTGG CGGGCGGGCG GTTTGACGCG 151 CATGATTTTG TTGGAGGCGT ATTGTCTGCG GGCGCGGCGG CGGTTGTGGT 201 TTCGCGCGAA GATTGCGCGG CTTTGGGCGG CGCGTTGAAA GTCGATGACA 251 CGCTTGCCGC GTTGCAAACG TTGGCGAAGG CGTGGCGCGA TAATGTGAAC 301 CCGTTTGTGT TCGGCATTAC CGGTTCGGGC GGCAAGACGA CGGTGAAGGA 351 GATGCTGGCT GCGGTATTGC GCCGCCGTTT CGGCGATGAT GCCGTTTCGG 401 CGACGGCAGG CAACTTCAAC AACCACAtcg gaTTGCCGCT GACTTTATTG 451 AAATtaaAcg aAAAACACCG CTATGCCGTG ATTGAAATGG GCATGAACCA 501 TTTTGGcgaa ctggcggtTt taacgcaaaT CGCCAAACCC GATGCCGCTT 551 TGGtcaACAA CGCCCTGCGC GCCCATGTCG GATGCGGTTt cgacggagtg 601 GGCGATATTG CCAAAGcgaa aagcGAGATT TatgcagGct tATGTTCAGA 651 CGGCATGGCA CTGATTCCTC AAGAAGATGC CAATATGGCT GTCTTCAAAA 701 CGGCAACGTT TAATTTGAAT ACGTGCACTT TCGGCGTCGA TAGCGGCGAT 751 GTCCGCGCGG AAAATATCGT GCTGAAACCT TTGTCGTGCG AATTTGATTT 801 GGTGTGCGGC GACGAGCGCA CTGCCGTGGT GCTGCCTGTT CCCGGCCGCC 851 ACAATGTCCA CAACGCCGCC GCTGCCGCCG CGCTGGCTTT GGCTGCCGGT 901 TTGAGTTTGA ACGATGTGGC GGAAGGTTTG CAAGGCTTCA GCAACATCAA 951 AGGCCGTCTG AACGTCAAAG CCGGCATCAA GGGCGCAACC CTGATTGACG 1001 ATACTTATAA TGCGAATCCC GACAGTATGA AAGCCGCGGT TGACGTGTTG 1051 GCGCGTATGC CTGCGCCGCG CATTTTCGTG ATGGGCGATA TGGGCGAACT 1101 GGGCGAGGAc gaAGCCGCCG CCATGCACGC CGAagtcgGC GCGTACGCCC 1151 GCGACCAAGG CATCGAAGCG GCTTATTTTG TCGGCGACAA CAGCGTCGAA 1201 GCGGcggaAA AATTTGGCGC GGACGGTTTG TGGTTCGCCG CCAAAGACCC 1251 GTTGATTCAA GTGTTGAGCC ACGATTTGCC CGAACGCGCC ACCGTGTTGG 1301 TGAAAGGTTC GCGCTTTATG CAGAtggAAG AAGTGGTCGA GGCATTGGAG 1351 GATAAGTga This corresponds to the amino acid sequence <SEQ ID 248; ORF 081.ng>: gOδl.pep 1 MKPLDLNFIC QALKLPMPSE NKPVSRIVTD SRDIREGDVF FALAGGRFDA 51 HDFVGGVLSA GAAAVWSRE DCAALGGALK VDDTLAALQT LAKAWRDNVN 101 PFVFGITGSG GKTTVKEMLA AVLRRRFGDD AVSATAGNFN NHIGLPLTLL 151 KLNEKHRYAV IEMGMNHFGE LAVLTQIAKP DAALVNNALR AHVGCGFDGV 201 GDIAKAKSEI YAGLCSDGMA LIPQEDANMA VFKTATFNLN TCTFGVDSGD 251 VRAENIVLKP LSCEFDLVCG DERTAWLPV PGRHNVHNAA AAAALALAAG 301 LSLNDVAEGL QGFSNIKGRL NVKAGIKGAT LIDDTYNANP DSMKAAVDVL 351 ARMPAPRIFV MGDMGELGED EAAAMHAEVG AYARDQGIEA AYFVGDNSVE 401 AAEKFGADGL WFAAKDPLIQ VLSHDLPERA TVLVKGSRFM QMEEWEALE 451 DK* The following partial DNA sequence was identified in N meningitidis <SEQ ID 249>: m081.seq 1 ATGAAACCAC TGGACCTAAA TTTCATCTGC CAAGCCCTCA AGCTTCCGAT 51 GCCGTCTGAA AGCAAACCCG TGTCGCGCAT CGTAACCGAC AGCCGCGACA 101 TCCGCGCGGG CGATGTGTTT TTCGCATTGG CGGGCGAGCG GTTTGACGCG 151 CATGATTTTG TTGAAGACGT ATTGGCTGCT GGTGCGGCGG CGGTTGTGGT 201 TTCGCGCGAA GATTGTGCTG CAATGGATGG CGCGTTGAAA GTCGATGACA 251 CGCTTGCCGC ATTGCAAACG CTGGCAAAGG CGTGGCGTGA AAATGTGAAT 301 CCGTTTGTGT TCGGCATTAC CGGTTCGGGC GGCAAGACGA CGGTGAAGGA 351 AATGCTGGCT GCGGTATTGC GCCgCCGTTT CGGCGATGAT GCCGTGTTGG 401 CGACGGCAGG CAACTTCAAC AACCATATCG GATTGCCGCT GACTTTGTTG 451 AAGTTAAACG AAAAACACCG CTATGCCGTG ATTGAAATGG GCATGAACCA 501 TTTCGGCGAA CTGGCGGTTT TAACGCAmAT CGCCAAACCA AATGCCGCAT 551 TGGTCAACAA CGCCATGCGC GCCCATGTCG GCTGCGGTTT CGACGGAGTG 601 GGCGATATTG CCAAAGCGAA AAGCGAGATT TACCAAGGTT TATGTTCAGA 651 CGGCATTGCA CTGATTCCTC AAGAAGATGC CAATATGGCT GTCTTCAAAA 701 CGGCAACGCT TAATTTGAAT ACGCGCACTT TCGGCATCGA TAGCGGCGAT 751 GTTCACGCGG AAAATATTGT GCTGAAACCG TTGTCGTGCG AATTTGATTT 801 GGTGTGCGGC GATGAGCGCG CCGCCGTGGT GCTGCCTGTT CCCGGCCGCC 851 ACAATGTCCA CAACGCCGCC GCTGCCGCCG CGCTGGCTTT GGCTGCGGGT 901 TTGAGTTTGA ACGATGTGGC GGAAGGTTTG AAAGGCTTCA GCAATATCAA 951 AGGCCGTCTG AACGTCAAAT CCGGAATCAA GGGCGCAACC CTGATTGACG 1001 ATACTTATAA TGCGAACCCT GACAGCATGA AAGCTGCGAT TGACGTGTTG 1051 GCGCGTATGC CTGCGCCGCG TATTTTCGTG ATGGGCGATA TGGGCGAACT 1101 GGGCGAACTG GGCGAGGACG AAGCCGCCGC TATGCACGCC GAAGTCGGCG 1151 CGTATGCCCG CGACCAAGGC ATCGAAGCGG CTTATTTTGT CGGCGACAAC 1201 AGCGTCGAAG CGGCGGAAAA ATTTGGCGCG GACGGTTTGT GGTTCGCCGC 1251 CAAAGACCCG TTGATTCAAG TGTTGCGCCA CGATTTGCCC GAACGCGCCA 1301 CCGTGTTGGT GAAAGGTTCG CGCTTTATGC AGATGGAAGA AGTGGTCGAG 1351 GCATTGGAGG ATAAGTGA This corresponds to the amino acid sequence <SEQ ID 250; ORF 081>: mOδl.pep 1 MKPLDLNFIC QALKLPMPSE SKPVSRIVTD SRDIRAGDVF FALAGERFDA 51 HDFVEDVLAA GAAAVWSRE DCAAMDGALK VDDTLAALQT LAKAWRENVN 101 PFVFGITGSG GKTTVKEMLA AVLRRRFGDD AVLATAGNFN NHIGLPLTLL 151 KLNEKHRYAV IEMGMNHFGE LAVLTXIAKP NAALVNNAMR AHVGCGFDGV 201 GDIAKAKSEI YQGLCSDGIA LIPQEDANMA VFKTATLNLN TRTFGIDSGD 251 VHAENIVLKP LSCEFDLVCG DERAAWLPV PGRHNVHNAA AAAALALAAG 301 LSLNDVAEGL KGFSNIKGRL NVKSGIKGAT LIDDTYNANP DSMKAAIDVL 351 ARMPAPRIFV MGDMGELGEL GEDEAAAMHA EVGAYARDQG IEAAYFVGDN 401 SVEAAEKFGA DGLWFAAKDP LIQVLRHDLP ERATVLVKGS RFMQMEEWE 451 ALEDK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 081 shows 94.1% identity over a 455 aa overlap with a predicted ORF (ORF 08 l.ng) from N gonorrhoeae: m081/g081 10 20 30 40 50 60 m081.pep MKPLDLΝFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALAGERFDAHDFVEDVLAA MMMMMMMMMMMMMIMMMM MM g081 MKPLDLΝFICQALKLPMPSEΝKPVSRIVTDSRDIREGDVFFALAGGRFDAHDFVGGVLSA 10 20 30 40 50 60 70 80 90 100 110 120 m081.pep GAAAWVSREDCAAMDGALKVDDTLAALQTLAKAWREΝVΝPFVFGITGSGGKTTVKEMLA MMMMMMIh MMIMMMMMMMIMMMMMMMMMMMM g081 GAAAVWSREDCAALGGALKVDDTLAALQTLAKAWRDΝVΝPFVFGITGSGGKTTVKEMLA 70 80 90 100 110 120 130 140 150 160 170 180 O 81. pep AVLRRRFGDDAVLATAGΝFΝΝHIGLPLTLLKLΝEKHRYAVIEMGMΝHFGELAVLTXIAKP 111111111111 MMMMMMMMMMMMMMMMMMMMM MM g081 AVLRRRFGDDAVSATAGΝFΝΝHIGLPLTLLKLΝEKHRYAVIEMGMΝHFGELAVLTQIAKP 130 140 150 160 170 180 190 200 210 220 230 240 m081.pep ΝAALVΝΝAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDGIALIPQEDAΝMAVFKTATLΝLΝ MMMMMMMMMMIMMMMM I II II h II II II I II II II II I h II I g081 DAALVΝΝALRAHVGCGFDGVGDIAKAKSEIYAGLCSDGMALIPQEDAΝMAVFKTATFΝLΝ 190 200 210 220 230 240 250 260 270 280 290 300 m081.pep TRTFGIDSGDVHAEΝIVLKPLSCEFDLVCGDERAAVVLPVPGRHΝVHΝAAAAAALALAAG I MhlMMMMMMMMMMMMMMMMMMMMMMIMMMM g081 TCTFGVDSGDVRAEΝIVLKPLSCEFDLVCGDERTAVVLPVPGRHΝVHΝAAAAAALALAAG 250 260 270 280 290 300 310 320 330 340 350 360 m081.pep LSLΝDVAEGLKGFSΝIKGRLΝVKSGIKGATLIDDTYΝAΝPDSMKAAIDVLARMPAPRIFV 11111111 II = I i 111 M 1111 11111 II 111 II 111111111 hi I i 111 M 11111 gO 81 LSLΝDVAEGLQGFSΝIKGRLΝVKAGIKGATLIDDTYΝAΝPDSMKAAVDVLARMPAPRIFV 310 320 330 340 350 360 370 380 390 400 410 420 mO81. pep MGDMGELGELGEDEAAAMHAEVGAYARDQGIEAAYFVGDΝSVEAAEKFGADGLWFAAKDP MIIIIIM MMMMMMMMMMMMMMMIMMMIMMMMM g081 MGDMGELGE DEAAAMHAEVGAYARDQGIEAAYFVGDΝSVEAAEKFGADGLWFAAKDP 370 380 390 400 410 430 440 450 mO81. pep LIQVLRHDLPERATVLVKGSRFMQMEEWEALEDKX Mill g081 LIQVLSHDLPERATVLVKGSRFMQMEEWEALEDKX 420 430 440 450 The following partial DNA sequence was identified in N meningitidis <SEQ ID 251>: aOδl.seq 1 ATGAAACCAC TGGACCTAAA TTTCATCTGC CAAGCCCTCA AGCTTCCGAT 51 GCCGTCTGAA AGCAAACCCG TGTCGCGCAT CGTAACCGAC AGCCGCGACA 101 TCCGCGCGGG CGATGTGTTT TTCGCATTGG CGGGCGGGCG GTTTGATGCG 151 CATGATTTTG TTGAAGACGT ATTGGCTGCG GGTGCGGCGG CGGTTGTGGT 201 TTCGCGCGAA GATTGCGTTG CAATGGATGG CGCGTTGAAA GTCGATGACA 251 CGCTTACCGC GTTGCAAATG TTGGCGAAGG CGTGGCGCGA GAATGTGAAC 301 CCGTTTGTGT TCGGTATTAC CGGCTCGGGC GGCAAGACGA CGGTGAAGGA 351 AATGTTGGCT GCGGTATTGC GCCGCCGTTT CGGCGATAAT GCCGTTTTGG 401 CGACGGCAGG CAACTTCAAC AACCACATCG GATTGCCGTT GACTTTGTTG 451 AAATTAAACG AAAAACACCG CTATGCCGTG ATTGAAATGG GTATGAACCA 501 TTTTGGCGAA CTGGCGGTTT TGACACAAAT CGCCAAACCC GATGCCGCAT 551 TGGTCAACAA CGCCATGCGC GCCCATGTCG GCTGCGGTTT CGACGGAGTG 601 GGCGATATTG CCAAAGCGAA AAGCGAGATT TATCAAGGCT TATGTTCAGA 651 CGGCATGGCG CTGATTCCTC AAGAAGATGC CAATATGGCT GTCTTCAAAA 701 CGGCAACGCT TAATTTGAAT ACGCGCACTT TCGGCATCGA TAGCGGCGAT 751 GTCCACGCGG AAAATATCGT GCTGAAACCG TTGTCGTGCG AATTTGATTT 801 GGTGTGCGGC AACGAGTGCG CAGCCGTGGT TCTGCCCGTT CCCGGCCGCC 851 ACAATGTCCA CAACGCCGCC GCCGCCGCCG CGCTGTCTTT GGCTGCAGGT 901 TTGAGTTTGA ACGATGTGGC GGAAGGTTTG AAAGGCTTCA GCAATATCAA 951 AGGCCGTCTG AACGTCAAAT CCGGAATCAA GGGCGCAACC CTGATTGACG 1001 ATACTTATAA TGCGAACCCT GACAGCATGA AAGCTGCGGT TGACGTGTTG 1051 GCGCGTATGC CTGCGCCGCG TATTTTCGTG ATGGGCGATA TGGGCGAACT 1101 GGGTGAGGAC GAAGCCGCCG CCATGCACGC CGAAGTCGGC GCGTACGCCC 1151 GCGACCAAGG CATCGAAGCG GCTTATTTTG TCGGCGACAA CAGCGTCGAA 1201 GCGGCGGAAA AATTTGGCGC GGACGGTTTG TGGTTCGCCG CCAAAGACCC 1251 GTTGATTCAA GTGTTGCGCC ACGATTTGCC CGAACGCGCC ACCGTGTTGG 1301 TGAAAGGTTC GCGCTTTATG CAGATGGAAG AAGTGGTCGA GGCATTGGAG 1351 GATAAGTGA This corresponds to the amino acid sequence <SEQ ID 252; ORF 081.a>: aOδl.pep 1 MKPLDLNFIC QALKLPMPSE SKPVSRIVTD SRDIRAGDVF FALAGGRFDA 51 HDFVEDVLAA GAAAVWSRE DCVAMDGALK VDDTLTALQM LAKAWRENVN 101 PFVFGITGSG GKTTVKEMLA AVLRRRFGDN AVLATAGNFN NHIGLPLTLL 151 KLNEKHRYAV IEMGMNHFGE LAVLTQIAKP DAALVNNAMR AHVGCGFDGV 201 GDIAKAKSEI YQGLCSDGMA LIPQEDANMA VFKTATLNLN TRTFGIDSGD 251 VHAENIVLKP LSCEFDLVCG NECAAWLPV PGRHNVHNAA AAAALSLAAG 301 LSLNDVAEGL KGFSNIKGRL NVKSGIKGAT LIDDTYNANP DSMKAAVDVL 351 ARMPAPRIFV MGDMGELGED EAAAMHAEVG AYARDQGIEA AYFVGDNSVE 401 AAEKFGADGL WFAAKDPLIQ VLRHDLPERA TVLVKGSRFM QMEEVVEALE 451 DK* m08i/a08i 96.1% identity over a 455 aa overlap 10 20 30 40 50 60 m081.pep MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALAGERFDAHDFVEDVLAA I II I II I I I I I I II II II I II II II II I I II II I II II II II I II II II II I I II II II a081 MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALAGGRFDAHDFVEDVLAA 10 20 30 40 50 60 70 80 90 100 110 120 m081. pep GAAAWVSREDCAAMDGALKVDDTLAALQTLAKAWRENVNPFVFGITGSGGKTTVKEMLA I II I II I II II I : I II I II II I I II : II I II II II II I I II II II II II II I II I II II a081 GAAAWVSREDCVAMDGALKVDDTLTALQMLAKAWRENVNPFVFGITGSGGKTTVKEMLA 70 80 90 100 110 120 130 140 150 160 170 180 m081.pep AVLRRRFGDDAVLATAGNFNNHIGLPLTLLKLNEKHRYAVIEMGMNHFGELAVLTXIAKP II I II II II : I I II II II II II I I II II II II I II II II I II II I I I I II II II I II II a081 AVLRRRFGDNAVLATAGNFNNHIGLPLTLLKLNEKHRYAVIEMGMNHFGELAVLTQIAKP 130 140 150 160 170 180 190 200 210 220 230 240 m081. pep NAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDGIALIPQEDANMAVFKTATLNLN : I I II II I II II II II II II II I II II II I II II I II I : II II II I I I I I I I I I I I II II a081 DAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDGMALIPQEDANMAVFKTATLNLN 190 200 210 220 230 240 250 260 270 280 290 300 m081.pep TRTFGIDSGDVHAENIVLKPLSCEFDLVCGDERAAWLPVPGRHNVHNAAAAAALALAAG I II I II II II II I I II II I I I I II II II II : I I II II I I I I II I II II II I I I I : II I I a081 TRTFGIDSGDVHAENIVLKPLSCEFDLVCGNECAAWLPVPGRHNVHNAAAAAALSLAAG 250 260 270 280 290 300 310 320 330 340 350 360 m081.pep LSLNDVAEGLKGFSNIKGRLNVKSGIKGATLIDDTYNANPDSMKAAIDVLARMPAPRIFV II II I II II I I II II I II II I II II I II I I II I II II I II II II II : II II I I I I I II I I a081 LSLNDVAEGLKGFSNIKGRLNVKSGIKGATLIDDTYNANPDSMKAAVDVLARMPAPRIFV 310 320 330 340 350 360 370 380 390 400 410 420 m081.pep MGDMGELGELGEDEAAAMHAEVGAYARDQGIEAAYFVGDNSVEAAEKFGADGLWFAAKDP II II I II I I II II I I II II I II II II I II II II II II II II I II II II II II I I II I a081 MGDMGELGE DEAAAMHAEVGAYARDQGIEAAYFVGDNSVEAAEKFGADGLWFAAKDP 370 380 390 400 410 430 440 450 m081.pep LIQVLRHDLPERATVLVKGSRFMQMEEWEALEDKX II I II II I II II I I II II II II I II II II I II II II a081 LIQVLRHDLPERATVLVKGSRFMQMEEWEALEDKX 420 430 440 450 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 253>: g082.seq 1 aTGTGGTTGT TGAAGTTGCC TGCCGTCGCC GAAACGGCAT CATCGCCGAA 51 ACGGCGGCGC AATACCGCAG CCAGCATCTC CTTCACCGTC GTCTTGCCGC 101 CCGAACCGGT AATGCCGAAC ACAAACGGGT TCACATTATC GCGCCACGCC 151 TTCGCCAACG TTTGCAACGC GGCAAGCGTG TCATCGACTT TCAACGCGCC 201 GCCCAAAGCC GCGCAATCTT CGCGCGAAAC CACAACCGCC GCCGCGCCCG 251 CAGACAATAC GCCTCCAACA AAATCATGCG CGTCAAACCG CCCGCCCGCC 301 AATGCGAAAA ACACATCGCC TTCCCGAATA TCGCGGCTGT CGGTTACGAT 351 GCGCGACACG GGTTTGTTTT CAGACGGCAT CGGAAGCTTG AGGGCTTGGC 401 AGATGAAATT TAGGTCCAGT GGTTTCATAT TTGCTTTCGT TAATATTCGG 451 GCGGCGGACA CATCGGTAGC GGCTGATTTT TTTATCGCCT GTTTTGCTGT 501 GGTAAAACAC AGATTATTTT CCCATTCTCA TTCGGCATTT TTTCTGTACG 551 TATCATTTTT TAGACGTATT TTTAGCCGAT TTGCCTTTTC CCGCATACCA 601 CGGCGCGGGG TCGTCGGACT GTCTGTCGAT AAAGGCAAGG TTATTGCCTT 651 CGCCCGGCAC ATCGGGGACA TTCCCCCAAA AATCATAGCC GTCATCGGGC 701 AACTCGTCGG TTTCGATACC CGTCCAACTG CCGAATCCGC GTAA This corresponds to the amino acid sequence <SEQ ID 254; ORF 082.ng>: g082.pep 1 MWLLKLPAVA ETASSPKRRR ΝTAASISFTV VLPPEPVMPΝ TΝGFTLSRHA 51 FAΝVCΝAASV SSTFΝAPPKA AQSSRETTTA AAPADΝTPPT KSCASΝRPPA 101 ΝAKΝTSPSRI SRLSVTMRDT GLFSDGIGSL RAWQMKFRSS GFIFAFVΝIR 151 AADTSVAADF FIACFAWKH RLFSHSHSAF FLYVSFFRRI FSRFAFSRIP 201 RRGWGLSVD KGKVIAFARH IGDIPPKIIA VIGQLVGFDT RPTAESA* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 255>: m082. seq 1 ATGnnGTTGT TGAAGTTGCC TGCCGTCGCC AACACGGCAT CATCGCCGAA 51 ACGGcGGCGC AATACCGCAG CCAGCATTTC CTTCACCGTC GTCTTGCCGC 101 CCGAACCGGT AATGCCGAAC ACAAACGGAT TCACATTTTC ACGCCACGCC 151 TTTGCCAGCG TTTGCAATGC GGCAAGCGTG TCATCGACTT TCAACGCGCC 201 ATCCATTGCA GCACAATCTT CGCGCGAAAC CACAACCGCC GCCGCACCAG 251 CAGCCAATAC GTCTTCAACA AAATCATGCG CGTCAAACCG CTCGCCCGCC 301 AATGCGAAAA ACACATCGCC CGCGCGGATG TCGCGGCTGT CGGTTACGAT 351 GCGCGACACG GGTTTGCTTT CAGACGGCAT CGGAAGCTTG AGGGCTTGGC 401 AGATGAAATT TAGGTCCAGT GGTTTCATAT TTACTTTCGT TAATATTCGG 451 GCGGCGGACA CATCGGTAGC GGCTGATTTT TTTATCGCCT GTTTTGCTGT 501 GGTAAAACAC AGATTATTTT CCCATTCTCA TTCGGsATTT TTTCTGTACG 551 TATCATTTTT TAGACGTATT TTTAGTCGAT TTGCCTTTTC CCGCATACCA 601 CGGCGCGGGG TCGTCGGGCA GTCCGTCGAT AAAGGCAAGG TTATTGCCTT 651 CGCCCTGCAC ATCGGGAACA TTCCCCCAAA AATCATAGCC GTCATCGGGC 701 AACTCGTCGG TTTCGATACC CGTCCAACTG CCGAATCCGC GTAA This corresponds to the amino acid sequence <SEQ ID 256; ORF 082>: m082.pep 1 MXLLKLPAVA NTASSPKRRR NTAASISFTV VLPPEPVMPN TNGFTFSRHA 51 FASVCNAASV SSTFNAPSIA AQSSRETTTA AAPAANTSST KSCASNRSPA 101 NAKNTSPARM SRLSVTMRDT GLLSDGIGSL RAWQMKFRSS GFIFTFVNIR 151 AADTSVAADF FIACFAWKH RLFSHSHSXF FLYVSFFRRI FSRFAFSRIP 201 RRGWGQSVD KGKVIAFALH IGNIPPKIIA VIGQLVGFDT RPTAESA* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 082 shows 92.7% identity over a 247 aa overlap with a predicted ORF (ORF 082.ng) from N. gonorrhoeae: m082/g082 10 20 30 40 50 60 m082.pep MXLLKLPAVAΝTASSPKRRRΝTAASISFTWLPPEPVMPΝTΝGFTFSRHAFASVCΝAASV I MMMMMMMMMMMMMMMMMMMMMIMMIMMMMM g082 MWLLKLPAVAETASSPKRRRΝTAASISFTWLPPEPVMPΝTΝGFTLSRHAFAΝVCΝAASV 10 20 30 40 50 60 70 80 90 100 110 120 m082.pep SSTFΝAPSIAAQSSRETTTAAAPAAΝTSSTKSCASΝRSPAΝAKΝTSPARMSRLSVTMRDT 11 ! 1111111 ! 1111 II IIIIIMI II II I II I h I M II 111 II 11 g082 SSTFΝAPPKAAQSSRETTTAAAPADΝTPPTKSCASΝRPPAΝAKΝTSPSRISRLSVTMRDT 70 80 90 100 110 120 130 140 150 160 170 180 m082.pep GLLSDGIGSLRAWQMKFRSSGFIFTFVΝIRAADTSVAADFFIACFAWKHRLFSHSHSXF MMMMMMMMMMMMMMMIMMMMMMMMMIMMMM I g082 GLFSDGIGSLRAWQMKFRSSGFIFAFVΝIRAADTSVAADFFIACFAWKHRLFSHSHSAF 130 140 150 160 170 180 190 200 210 220 230 240 m082.pep FLYVSFFRRIFSRFAFSRIPRRGWGQSVDKGKVIAFALHIGΝIPPKIIAVIGQLVGFDT M M M M I M M I M M M M M M 1 1 ! 1 1 i 1 1 1 1 ! I I I M I I I 1 1 I I I I I I I I I I I g082 FLYVSFFRRIFSRFAFSRIPRRGWGLSVDKGKVIAFARHIGDIPPKIIAVIGQLVGFDT 190 200 210 220 230 240 m082.pep RPTAESAX IIIIIMI g082 RPTAESAX The following partial DΝA sequence was identified in N meningitidis <SEQ ID 257>: a082.seq 1 ATGTGGTTGT TGAAGTTGCC TGCCGTCGCC AAAACGGCAT TATCGCCGAA 51 ACGGCGGCGC AATACCGCAG CCAACATTTC CTTCACCGTC GTCTTGCCGC 101 CCGAGCCGGT AATACCGAAC ACAAACGGGT TCACATTCTC GCGCCACGCC 151 TTCGCCAACA TTTGCAACGC GGTAAGCGTG TCATCGACTT TCAACGCGCC 201 ATCCATTGCA ACGCAATCTT CGCGCGAAAC CACAACCGCC GCCGCACCCG 251 CAGCCAATAC GTCTTCAACA AAATCATGCG CATCAAACCG CCCGCCCGCC 301 AATGCGAAAA ACACATCGCC CGCGCGGATG TCGCGGCTGT CGGTTACGAT 351 GCGCGACACG GGTTTGCTTT CAGACGGCAT CGGAAGCTTG AGGGCTTGGC 401 AGATGAAATT TAGGTCCAGT GGTTTCATAT TTACTTTCGT TAATATTCGG 451 GCGGCGGACA CATCGGTAGC GGCTGATTTT TTTATCGCCT GTTTTGCTGT 501 GGTAAAACAC AGATTATTTT CCCATTCTCA TTCGGCATTT TTTCTGTACG 551 TATCATTTTT TAGACGTATT TTTAGTCGAT TTGCCTTTTC CCGCATACCA 601 CGGCGCGGGG TCGTCGGGCA GTCCGTCGAT AAAGGCAAGG TTATTGCCTT 651 CGCCCTGCAC ATCGGGAACA TTCCCCCAAA AATCATAGCC GTCATCGGGC 701 AACTCGTCGG TTTCGATACC CGTCCAACTG CCGAATCCGC GTAA This corresponds to the amino acid sequence <SEQ ID 258; ORF 082. a>: a082.pep 1 MWLLKLPAVA KTALSPKRRR NTAANISFTV VLPPEPVIPN TNGFTFSRHA 51 FANICNAVSV SSTFNAPSIA TQSSRETTTA AAPAANTSST KSCASNRPPA 101 NAKNTSPARM SRLSVTMRDT GLLSDGIGSL RAWQMKFRSS GFIFTFVNIR 151 AADTSVAADF FIACFAWKH RLFSHSHSAF FLYVSFFRRI FSRFAFSRIP 201 RRGVVGQSVD KGKVIAFALH IGNIPPKIIA VIGQLVGFDT RPTAESA* m082/a082 95.5%ι identity over a 247 aa overlap 10 20 30 40 50 60 m082.pep MXLLKLPAVANTASSPKRRRNTAASISFTWLPPEPVMPNTNGFTFSRHAFASVCNAASV I II II I II I: I I II II II II I I : II I II I II II I I : I I I I II II II II I I :: I II : I I a082 MWLLKLPAVAKTALSPKRRRNTAANISFTWLPPEPVIPNTNGFTFSRHAFANICNAVSV 10 20 30 40 50 60 70 80 90 100 110 120 m082. pep SSTFNAPSIAAQSSRETTTAAAPAANTSSTKSCASNRSPANAKNTSPARMSRLSVTMRDT I I I II I I II I : I I II II I II I II II II I II I II II II I II II I II II II I II I I II II I a082 SSTFNAPSIATQSSRETTTAAAPAANTSSTKSCASNRPPANAKNTSPARMSRLSVTMRDT 70 80 90 100 110 120 130 140 150 160 170 180 m082.pep GLLSDGIGSLRAWQMKFRSSGFIFTFVNIRAADTSVAADFFIACFAWKHRLFSHSHSXF I II II II I II II II II I II I II II II II I I II I I II II I I I II I II II II I II II I II I a082 GLLSDGIGSLRAWQMKFRSSGFIFTFVNIRAADTSVAADFFIACFAWKHRLFSHSHSAF 130 140 150 160 170 180 190 200 210 220 230 240 m082.pep FLYVSFFRRIFSRFAFSRIPRRGVVGQSVDKGKVIAFALHIGNIPPKIIAVIGQLVGFDT I I I II I I I II I II II II II I I II II II I I II II II II I II I I I II II I I I II I II I I I I I a082 FLYVSFFRRIFSRFAFSRIPRRGWGQSVDKGKVIAFALHIGNIPPKIIAVIGQLVGFDT 190 200 210 220 230 240 m082.pep RPTAESAX I I I I II I I a082 RPTAESAX The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 259>: g084. seq 1 ATGAAacaAT CCGcccgaat aAAAAATATG GATCAGACAT TAAAAAATAc 51 attgggcatt tGCGCGcttt tagcctTTTG TTTTggcgcG gccaTCGCAT 101 CAGGTTATCA CTTGGAATAT GAATACGGCT ACCGTTATTC TGCCGTGGGC 151 GCTTTGGCTT CGGTTGTATT TTTATTATTA TTGGCACGCG GCTTCCCGCG 201 CGTTTCTTCA GTTGTTTTAC TGATTTACGT CGGCACAACC GCCCTATATT 251 TGCCGGTCGG CTGGCTGTAT GGTGCGCCTT CTTATCAGAT AGTCGGTTCG 301 ATATTGGAAA GCAATCCTGC CGAGGCGCGT GAATTTGTCG GCAATCTTCC 351 CGGGTCGCTT TATTTTGTGC AGGCATTATT TTTCATTTTT GGCTTGACAG 401 TTTGGAAATA TTGTGTATCT GTGGGGGTAT TTGCTGACGT AAAAAACTAT 451 AAACGTCGCA GCAAAATATG GCTGACCATA TTATTGACTT TGATTTTGTC 501 CTGCGCGGTG ATGGAGAAAA TCGccggcga taaAGATTGG CGAGaacctg 551 atgccggcct gttgttgaat ATTTTcgacc tgtattaCga cttggctttc 601 cgcgccggca cAATATGCCG CCAAGCGCGC CCAcattttg gaagCagcaa 651 aaaaagcgtC AACATGGCAt atccgccaac ttgcgcccaa gTAtaa This corresponds to the amino acid sequence <SEQ ID 260; ORF 084.ng>: g084.pep 1 MKQSARIKNM DQTLKNTLGI CALLAFCFGA AIASGYHLEY EYGYRYSAVG 51 ALASWFLLL LARGFPRVSS WLLIYVGTT ALYLPVGWLY GAPSYQIVGS 101 ILESNPAEAR EFVGNLPGSL YFVQALFFIF GLTVWKYCVS VGVFADVKNY 151 KRRSKIWLTI LLTLILSCAV MEKIAGDKDW REPDAGLLLN IFDLYYDLAF 201 RAGTICRQAR PHFGSSKKSV NMAYPPTCAQ V* The following partial DNA sequence was identified in N meningitidis <SEQ ID 261>: m084. seq 1 ATGAAACAAT CCGCCcGAAT AAAa.ATATG AATCAGACAT TACTTTATAC 51 ATTGGGCATT TGCGCGCTTT TAACCTTTnn nnnnnnnnnn nnnnnnnnnn 101 nnnnnTATCA CCCnGAATAT GAATACGGCT ACCGTTATTC TGCCGTGGGT 151 GCTTTGGCTT CGGTTGTATT TTTATTATTA TTGGCACGCG GTTTCCCGCG 201 CGTTTCTTCA GTTGTTTTAC TGATTTACGT CGGCACAACC GCCCTATATT 251 TGCCGGTCGG CTGGCTGTAT GGTGCGCCGT CTTATCAGAT AGTCGGTTCG 301 ATATTGGAAA GCAATCCTGC CGAGGCGCGT GAATTTGTCG GCAATCTTCC 351 CGGGTCGCTT TATTTTGTGC AGGCATTATT TTTCATTTTT GGCTTGACAG 401 TTTGGAAATA TTGTGTATCG GGGGGGGTAT TTGCTGACGT AAAAAACTAT 451 AAACGCCGCA GCAAAATATG GCTGACTATA TTATTGACTT TGATTTTGTC 501 CTGCGCGGTG ATGGATAAAA TCGCCAGCGA TAAAGATTTG CGAGAACCTG 551 ATGCCGGCCT GTTGTTGAAT ATTTTCGACC TGTATTACGA TTTGGCT.TC 601 CGCGCCGGCA CAATATGCCG CCAAGCGCGC CCACATTTTG GAAGCAGCAA 651 AAAAAGCGTC AACATGGCAT ATCCGTCATG TTGCGCCCAA GTATAA This corresponds to the amino acid sequence <SEQ ID 262; ORF 084>: m084.pep 1 MKQSARIKXM ΝQTLLYTLGI CALLTFXXXX XXXXXYHPEY EYGYRYSAVG 51 ALASWFLLL LARGFPRVSS WLLIYVGTT ALYLPVGWLY GAPSYQIVGS 101 ILESNPAEAR EFVGNLPGSL YFVQALFFIF GLTVWKYCVS GGVFADVKNY 151 KRRSKIWLTI LLTLILSCAV MDKIASDKDL REPDAGLLLN IFDLYYDLAX 201 RAGTICRQAR PHFGSSKKSV NMAYPSCCAQ V* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 084 shows 90.5% identity over a 231 aa overlap with a predicted ORF (ORF 084.ng) from N. gonorrhoeae: m084/g084 10 20 30 40 50 mO84. pep MKQSARIKXMΝQTLLYTLGICALLTF YHPEYEYGYRYSAVGALASWFLLL Mi IMM MIIMIhl II MMMMMMMMMMM g084 MKQSARIKΝMDQTLKΝTLGICALLAFCFGAAIASGYHLEYEYGYRYSAVGALASWFLLL 10 20 30 40 50 60 60 70 80 90 100 110 m084.pep LARGFPRVSSWLLIYVGTTALYLPVGWLYGAPSYQIVGSILESΝPAEAREFVGΝLPGSL MMMIMMMMMMMMMMIMMMMMMMMMMMMIIMMM g084 LARGFPRVSSWLLIYVGTTALYLPVGWLYGAPSYQIVGSILESΝPAEAREFVGΝLPGSL 70 80 90 100 110 120 120 130 140 150 160 170 m084.pep YFVQALFFIFGLTVWKYCVSGGVFADVKΝYKRRSKIWLTILLTLILSCAVMDKIASDKDL MMMMMMMMMM I II 1111 II 111 I II 11 II I II 11 II I I II M II M II g084 YFV Q ALFFIFGLTVWKYCVSVGVFADVKΝYKRRSKIWLTILLTLILSCAVMEKIAGDKDW 130 140 150 160 170 180 180 190 200 210 220 m084.pep REPDAGLLLNIFDLYYDLAXRAGTICRQARPHFGSSKKSVNMAYPSCCAQVX 1 1 1 i I 1 1 1 1 1 1 1 1 1 1 1 1 1 1 M M M M M M M M M I M M M M i l l g084 REPDAGLLLNIFDLYYDLAFRAGTICRQARPHFGSSKKSVNMAYPPTCAQVX 190 200 210 220 230 The following partial DNA sequence was identified in N meningitidis <SEQ ID 263>: a084.seq 1 ATGAAACAAT CCGCCCGAAT AAAAAATATG GATCAGACAT TAAAAAATAC 51 ATTGGGCATT TGCGCGCTTT TAGCCTTTTG TTTTGGCGCG GCCATCGCAT 101 CAGGTTATCA CTTGGAATAT GAATACGGCT ACCGTTATTC TGCCGTGGGT 151 GCTTTGGCTT CGGTTGTATT TTTATTATTA TTGGCACGCG GTTTCCCGCG 201 CGTTTCTTCA GTTGTTTTAC TGATTTACGT CGGCACAACC GCCCTATATT 251 TGCCGGTCGG CTGGCTGTAT GGTGCGCCGT CTTATCAGAT AGTCGGTTCG 301 ATATTGGAAA GCAATCCTGC CGAGGCGCGT GAATTTGTCG GCAATCTTCC 351 CGGGTCGCTT TATTTTGTGC AGGCATTATT TTTCATTTTT GGCTTGACAG 401 TTTGGAGATA TTGTGTATCG GGGGGGGTAT TTGCTGACGT AAAAAACTAT 451 AAACGCCGCA GCAAAATATG GCTGACTATA TTATTGACTT TGATTTTGTC 501 CTGCGCGGTG ATGGATAAAA TCGCCAGCGA TAAAGATTTG CGAGAACCTG 551 ATGCCGGCCT GTTGTTGAAT ATTTTCGACC TGTATTACGA TTTGGCTTCC 601 .GCGCCGGCA CAATATGCCG CCAAGCGCGC CCACATTTTG GAAGCAGCAA 651 AAAAAGCGTC AACATGGCAT ATCCGTCATG TTGCGCCCAA GTATAA This corresponds to the amino acid sequence <SEQ ID 264; ORF 084.a>: a084.pep 1 MKQSARIKΝM DQTLKΝTLGI CALLAFCFGA AIASGYHLEY EYGYRYSAVG 51 ALASWFLLL LARGFPRVSS WLLIYVGTT ALYLPVGWLY GAPSYQIVGS 101 ILESNPAEAR EFVGNLPGSL YFVQALFFIF GLTVWRYCVS GGVFADVKNY 151 KRRSKIWLTI LLTLILSCAV MDKIASDKDL REPDAGLLLN IFDLYYDLAS 201 XAGTICRQAR PHFGSSKKSV NMAYPSCCAQ V* m084/a084 92.2%> identity over a 231 aa overlap 10 20 30 40 50 60 O84. pep MKQSARIKXMNQTLLYTLGICALLTFXXXXXXXXXYHPEYEYGYRYSAVGALASWFLLL II I II II I I M I II I II II : I II I II I II II II II I I II II II I I a084 MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGYRYSAVGALASVVFLLL 10 20 30 40 50 60 70 80 90 100 110 120 m084.pep LARGFPRVSSWLLIYVGTTALYLPVGWLYGAPSYQIVGSILESNPAEAREFVGNLPGSL II I II I II II II II I I I II II I II II II II II I II I II I II I II I II I I II I II II II I I a084 LARGFPRVSSWLLIYVGTTALYLPVGWLYGAPSYQIVGSILESNPAEAREFVGNLPGSL 70 80 90 100 110 120 130 140 150 160 170 180 m084. pep YFVQALFFIFGLTVWKYCVSGGVFADVKNYKRRSKIWLTILLTLILSCAVMDKIASDKDL II I II I I II II II II II II I I I II II I I II I I II II I II I I I I II II II I II I I II II a08 YFVQALFFIFGLTVWRYCVSGGVFADVKNYKRRSKIWLTILLTLILSCAVMDKIASDKDL 130 140 150 160 170 180 190 200 210 220 230 m084.pep REPDAGLLLNIFDLYYDLAXRAGTICRQARPHFGSSKKSVNMAYPSCCAQVX II II II I II II II II II II I II II II II I II I II II I I II I I II II II I I a084 REPDAGLLLNIFDLYYDLASXAGTICRQARPHFGSSKKSVNMAYPSCCAQVX 190 200 210 220 230 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 265>: g085. seq 1 ATGGGCAAAG GGCAGGACTT CACGCCCCTG CGCGACGCGT TGAAAGATAA 51 GGCAAAAGGC GTGTTCCTGA TCGGCGTCGA TGCGCCGCAA ATCCGCCGCG 101 ATTTGGACGG CTGCGGCTTG AACCTGACCG ACTGCGTCAC TTTGGAAGAG 151 GCGGTTCAGA CGGCATACGC CCAAGCCGAA GCGGGCGATA TTGTCTTGCT 201 CAGCCCCGCC TGCGCGAGTT TCGATATGTT TAAAGGCTAC GCGCACCGTT 251 CGGAAGTGTT tatCGAAGCG TTTAAGGCTT TGTGA This corresponds to the amino acid sequence <SEQ ID 266; ORF 085.ng>: g085.pep 1 MGKGQDFTPL RDALKDKAKG VFLIGVDAPQ IRRDLDGCGL NLTDCVTLEE 51 AVQTAYAQAE AGDIVLLSPA CASFDMFKGY AHRSEVFIEA FKAL* The following partial DNA sequence was identified in N meningitidis <SEQ ID 267>: m085. seq 1 ATGGGTAAAG GGCAGGACTT CACGCCCCTG CGCGATGCAC TGGTAGGCAA 51 GGCAAAAGGC GTGTTCTTGA TTGGTGTCGA TGCGCCGCAA ATCCGCCGCG 101 ATTTGGACGG CTGCGGCTTG AATATGACCG ACTGCGCCAC TTTGGGAGAA 151 GCCGTTCAGA CGGCATATGC CCAAGCCGAA GCAGGCGATA TTGTGTTGCT 201 CAGCCCCGCC TGCGCGAGCT TTGATATGTT CAAAGGCTAC GCGCACCGTT 251 CGGAAGTGTT TATCGAAGCG TTTAAGGCTT TGTGA This corresponds to the amino acid sequence <SEQ ID 268; ORF 085>: 085.pep 1 MGKGQDFTPL RDALVGKAKG VFLIGVDAPQ IRRDLDGCGL ΝMTDCATLGE 51 AVQTAYAQAE AGDIVLLSPA CASFDMFKGY AHRSEVFIEA FKAL* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 085 shows 94.7% identity over a 94 aa overlap with a predicted ORF (ORF 085.ng) from N gonorrhoeae: m085/g085 10 20 30 40 50 60 mO85. pep MGKGQDFTPLRDALVGKAKGVFLIGVDAPQIRRDLDGCGLΝMTDCATLGEAVQTAYAQAE M M M M M M M 1 1 I I I I I 1 1 I I I I I I I I I I I I I I I h I I I = I I l l l l l l l l l l l g085 MGKGQDFTPLRDALKDKAKGVFLIGVDAPQIRRDLDGCGLΝLTDCVTLEEAVQTAYAQAE 10 20 30 40 50 60 70 80 90 m085 . pep AGDIVLLSPACASFDMFKGYAHRSEVFIEAFKALX M M M M M M M M M M M M M I M M M M g085 AGDIVLLSPACASFDMFKGYAHRSEVFIEAFKALX 70 80 90 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 269>: a085. seq 1 ATGGGCAAAG GGCAGGACTT CACGCCCCTG CGCGACGCGC TTGCCGGCAA 51 GGCAAAAGGC GTGTTCCTGA TCGGTGTCGA TGCGCCGCAA ATCCGCCGCG 101 ATTTGGACGG CTGCGATCTG AATATGACCG ACTGCGCCAC TTTGGAAGAA 151 GCGGTTCAGA AGGCATATGC CCAAGCCGAA GCGGGCGATA TCGTGCTGCT 201 CAGCCCCGCC TGCGCGAGTT TCGATATGTT TAAAGGCTAC GCGCACCGTT 251 CGGAAGTGTT TATCGGGGCG TTTAAGGCTT TGTGA This corresponds to the amino acid sequence <SEQ ID 270; ORF 085. a>: a085.pep 1 MGKGQDFTPL RDALAGKAKG VFLIGVDAPQ IRRDLDGCDL ΝMTDCATLEE 51 AVQKAYAQAE AGDIVLLSPA CASFDMFKGY AHRSEVFIGA FKAL* m085 a085 94.1% identity over a 94 aa overlap 10 20 30 40 50 60 m085. pep MGKGQDFTPLRDALVGKAKGVFLIGVDAPQIRRDLDGCGLΝMTDCATLGEAVQTAYAQAE I I II II I II I II I I : II II II I II II I I I I II II II II I II I II II I II I I llllll a085 MGKGQDFTPLRDALAGKAKGVFLIGVDAPQIRRDLDGCDLNMTDCATLEEAVQKAYAQAE 10 20 30 40 50 60 70 80 90 m085. pep AGDIVLLSPACASFDMFKGYAHRSEVFIEAFKALX I I II II I II I II II II I II I II II I II I llllll a085 AGDIVLLSPACASFDMFKGYAHRSEVFIGAFKALX 70 80 90 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 271>: g086. seq 1 ATGGTGGTGC TGATGACGGC GTTCGGCCTG CTGATGATTT ATTCGGCTTC 51 TGTGTATTTG GCATCGAAGG AAGGCGGCGA TCAGTTTTTC TATTTGACCA 101 GGCAGGCGGG GTTCGTCGTT GCCGGCCTTA TAGCGAGCGG TTTTTTATGG 151 TTTCTTTGCA GGATGAGGAC ATGGCGGCGG CTTGTGCCGT GGATTTTTGC 201 CTTATCCGGC CTGTTGCTGG TAGCCGTATT GATTGCCGGG CGCGAAATCA 251 ATGGCGCGAC CCGTTGGATA CCTTTGGGTC CGTTGAATTT CCAGCCGACC 301 GAGCTGTTCA AGCTGGCAGT CATCCTTTAT TTGGCAAGCC TGTTCACGCG 351 CCGTGAAGAA GTGTTGCGCA GCATGGAAAG TTTGGGTTGG CAGTCGATTT 401 GGCGGGGGAC GGCCAACCTG ATTATGTCCG CCACCAATCC GCAGGCACGT 451 CGTGAAACAT TAGAAATGTA CGgcCGTTTC CGGGCGATCA TCCTGCCGAT 501 TATGCTGGTG GCGTTCGGTT TGGTGCTGAT AATGGTACAG CCGGATTTCG 551 GTTCGTTTGT CGTCATTACC GTCATTACCG TTGGAATGCT GTTTCTGGCA 601 GGATTGCCGT GGAAATATTT TTTTGTCCTG GTAGGCAGCG TCTTGGGTGG 651 GATGGTGCTG ATGATTACCG CCGCTCCCTA CCGTGTGCAG CGGGTAGTGG 701 CATTTTTGGA CCCGTGGAAA GACCCGCAGG GTGCCGGCTA CCAGCTTACC 751 CACTCTCTGA TGGCAATCGG GCGCGGAGAG TGGTTCGGTA TGGGTTTGGG 801 TGCGAGTTTG AGCAAACGCG GCTTTCTGCC GGAAGCGCAT ACCGATTTTA 851 TTTTTGCCAT CATCGCTGAA GAATTCGGCT TCTTCGGGAT GTGCGTGCTG 901 ATATTCTGTT ACGGCTGGCT GGTGGTGCGG GCGTTTTCCA TCGGCAAGCA 951 GTCGCGCGAT TTGGGtttgA CTTTCAACGC CTATATCGCT TCGGGTATCG 1001 GCATTTGGAT CGGTATCCAA AGTTTCTTCA ATATCGGTGT GAACATCGGT 1051 GCTTTGCCGA CCAAAGGTCT GACGctgCcg tTGATGTCCT ATGGcggTTC 1101 GTCAGTCTTT TTCATGCTGA TCAGCATGAT GCTGCTGTTG CGTATCGATT 1151 ATGAAAACCG CCAGAAAATG CGCGGTTACC GGGTGGAGTA AA This corresponds to the amino acid sequence <SEQ ID 272; ORF 086.ng>: g086.pep 1 MWLMTAFGL LMIYSASVYL ASKEGGDQFF YLTRQAGFW AGLIASGFLW 51 FLCRMRTWRR LVPWIFALSG LLLVAVLIAG REIΝGATRWI PLGPLΝFQPT 101 ELFKLAVILY LASLFTRREE VLRSMESLGW QSIWRGTAΝL IMSATΝPQAR 151 RETLEMYGRF RAIILPIMLV AFGLVLIMVQ PDFGSFWIT VITVGMLFLA 201 GLPWKYFFVL VGSVLGGMVL MITAAPYRVQ RWAFLDPWK DPQGAGYQLT 251 HSLMAIGRGE WFGMGLGASL SKRGFLPEAH TDFIFAIIAE EFGFFGMCVL 301 IFCYGWLWR AFSIGKQSRD LGLTFΝAYIA SGIGIWIGIQ SFFΝIGVΝIG 351 ALPTKGLTLP LMSYGGSSVF FMLISMMLLL RIDYEΝRQKM RGYRVE* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 273>: m086. seq 1 ATGGTGGTGC TGATGACGGC GTTCAGCCTG CTGATGATTT ATTCGGCTTC 51 TGTGTATTTG GCATCAAAAG AAGGCGGCGA TCAGTTTTTC TATTTGACCA 101 GACAGGCGGG GTTCGTCGTT GCCGGCTTGA TAGCGAGCGG TTTGTTATGG 151 TTTCTTTGCA GGATGAGGAC ATGGCGGCGG CTTGTGCCGT GGATTTTTGC 201 CCTATCCGGC CTGTTGCTGG TAGTCGTATT GATTGCCGGG CGCGAAATCA 251 ATGGCGCGAC CCGTTGGATA CCTTTGGGTC CGTTGAATTT CCAGCCGACc 301 GAGCTGTTCA AGCtGGCGGT CATCCTTTAT TTGGCAAGCC TGTTCACGCG 351 CCGTGAAGAA GTGTTGcGCA GCATGGAAAG TTTGGGTTGG CAGTCGATTT 401 GGCGGGGGAC GGCCAATCTG ATCATGTCCG CCACCAATCC GCAGrCACGT 451 CGTGAaACAT TAGAAATGTA CGGCCGTwTC CGGGCGATCA TCCTGCCGAT 501 TATGCTGGTG GCGTTCGGTT TGGTGCTGAT AATGGTACAG CCGGATTTCG 551 GTTCGTTTGT CGTCATTACC GTCATTGCCG TTGGAATGCT GTTTTTGGCA 601 GGATTGCCGT GGAAATATTT TTTCGTCCTG GTAGGCAGCG TCTTGGGCGG 651 GATGGTGCTG ATGATTACCG CCGCTCCCTA CCGTGTGCAG CGGGTAGTGG 701 CATTTTTGGA CCCGTGGAAA GACCCGCAGG GTGCCGGCTA CCAGCTTACC 751 CACTCTCTGA TGGCAATCGG GCGCGGAGAG TGGTTCGGTA TGGGTTTGGG 801 TGCGAGTTTG AGCAAACGCG GCTTTCTGCC GGAAGCGCAT ACCGATTTTA 851 TTTTTGCCAT CATCGCCGAA GAATTCGGTT TCTTCGGTAT GTGCGTGCTG 901 ATATTCTGTT ACGGCTGGCT GGTGGTGCGG GCGTTTTCCA TCGGCAAGCA 951 GTCGCGCGAT TTGGGTTTGA CTTTCAACGC CTATATCGCT TCGGGTATCG 1001 GCATTTGGAT CGG rTCCAA AGTTTCTTCA ATATCGGTGT GAACATCGGT 1051 GCTTTGCCGA mCAAAgGyCT GACGCyGCCG Tg.AtGTCC ATGGCGGTTC 1101 GTCAGTCTTT TTCATGCTGA TCAGCATGAT GCTGCTGTkG CGTATAGATT 1151 ATGAAAACCG CCGGAAAATG CGCGGTTATC GGGTGGAGTA A This corresponds to the amino acid sequence <SEQ ID 274; ORF 086>: m086.pep 1 MWLMTAFSL LMIYSASVYL ASKEGGDQFF YLTRQAGFW AGLIASGLLW 51 FLCRMRTWRR LVPWIFALSG LLLVWLIAG REINGATRWI PLGPLNFQPT 101 ELFKLAVILY LASLFTRREE VLRSMESLGW QSIWRGTANL IMSATNPQXR 151 RETLEMYGRX RAIILPIMLV AFGLVLIMVQ PDFGSFWIT VIAVGMLFLA 201 GLPWKYFFVL VGSVLGGMVL MITAAPYRVQ RWAFLDPWK DPQGAGYQLT 251 HSLMAIGRGE WFGMGLGASL SKRGFLPEAH TDFIFAIIAE EFGFFGMCVL 301 IFCYGWLWR AFSIGKQSRD LGLTFNAYIA SGIGIWIGXQ SFFNIGVNIG 351 ALPXKGLTXP XMSXGGSSVF FMLISMMLLX RIDYENRRKM RGYRVE* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 086 shows 96.7% identity over a 396 aa overlap with a predicted ORF (ORF 086.ng) from N gonorrhoeae: m086/g086 10 20 30 40 50 60 mO 86. pep MWLMTAFSLLMI YSASVYLASKEGGDQFFYLTRQAGFWAGLIASGLLWFLCRMRTWRR MMMMMMMMMMIMMMMMMMMMMMMMMMIMMMM g086 MWLMTAFGLLMI YSASVYLASKEGGDQFFYLTRQAGFWAGLIASGFLWFLCRMRTWRR 10 20 30 40 50 60 70 80 90 100 110 120 m086.pep LVPWIFALSGLLLVWLIAGREIΝGATRWIPLGPLΝFQPTELFKLAVILYLASLFTRREE MMMMMMIhlMMMMMMMMMIMMMMMMMMIMMMM g086 LVPWIFALSGLLLVAVLIAGREIΝGATRWIPLGPLΝFQPTELFKLAVILYLASLFTRREE 70 80 90 100 110 120 130 140 150 160 170 180 m086.pep VLRSMESLGWQSIWRGTAΝLIMSATΝPQXRRETLEMYGRXRAIILPIMLVAFGLVLIMVQ MMMMMMMMMMMMMM g086 VLRSMESLGWQSIWRGTAΝLIMSATΝPQARRETLEMYGRFRAIILPIMLVAFGLVLIMVQ 130 140 150 160 170 180 190 200 210 220 230 240 m086.pep PDFGSFWITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQRWAFLDPWK MMMMMIhlMMMMMMMMMMMMMMMMMMMMMMM g086 PDFGSFWITVITVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQRWAFLDPWK 190 200 210 220 230 240 250 260 270 280 290 300 mO 86. pep DPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFI FAI IAEEFGFFGMCVL MMMMMMMMMMMMMMMMMIMMMMMMMMMIMMM g086 DPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIAEEFGFFGMCVL 250 260 270 280 290 300 310 320 330 340 350 360 m086 . pep IFCYGWLWRAFSIGKQSRDLGLTFNAYIASGIGIWIGXQSFFNIGVNIGALPXKGLTXP M M M M M M M M I M I g086 IFCYGWLWRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGVNIGALPTKGLTLP 310 320 330 340 350 360 370 380 390 m086 . pep XMSXGGSSVFFMLISMMLLXRIDYENRRKMRGYRVEX I I gO 86 LMSYGGSSVFFMLISMMLLLRIDYENRQKMRGYRVEX 370 380 390 The following partial DNA sequence was identified in N meningitidis <SEQ ID 275>: a086. seq 1 ATGGTGGTGC TGATGACGGC GTTCAGCCTG CTGATGATTT ATTCGGCTTC 51 TGTGTATTTG GCATCAAAAG AAGGCGGCGA TCAGTTTTTC TATTTGACCA 101 GACAGGCGGG GTTCGTCGTT GCCGGCTTGA TAGCGAGCGG TTTGTTATGG 151 TTTCTTTGCA GGATGAGGAC ATGGCGGCGG CTTGTGCCGT GGATTTTTGC 201 CCTATCCGGC CTGTTGCTGG TAGTCGTATT GATTGCCGGG CGCGAAATCA 251 ATGGCGCGAC CCGTTGGATA CCTTTGGGTC CGTTGAATTT CCAGCCGACC 301 GAGCTGTTCA AGCTGGCGGT CATCCTTTAT TTGGCAAGCC TGTTCACGCG 351 CCGTGAAGAA GTGTTGCGCA GCATGGAAAG TTTGGGTTGG CAGTCGATTT 401 GGCGGGGGAC GGCCAATCTG ATCATGTCCG CCACCAATCC GCAGGCACGT 451 CGTGAAACAT TAGAAATGTA CGGCCGTTTC CGGGCGATCA TCCTGCCGAT 501 TATGCTGGTG GCGTTCGGTT TGGTGCTGAT AATGGTACAG CCGGATTTCG 551 GTTCGTTTGT CGTCATTACC GTCATTGCCG TTGGAATGCT GTTTTTGGCA 601 GGATTGCCGT GGAAATATTT TTTCGTCCTG GTAGGCAGCG TCTTGGGCGG 651 GATGGTGCTG ATGATTACCG CCGCTCCCTA CCGTGTGCAG CGGGTAGTGG 701 CATTTTTGGA CCCGTGGAAA GACCCGCAGG GTGCCGGCTA CCAGCTTACC 751 CACTCTCTGA TGGCAATCGG GCGCGGAGAG TGGTTCGGTA TGGGTTTGGG 801 TGCGAGTTTG AGCAAACGCG GCTTTCTGCC GGAAGCGCAT ACCGATTTTA 851 TTTTTGCCAT CATCGCCGAA GAATTCGGTT TCTTCGGTAT GTGCGTGCTG 901 ATATTCTGTT ACGGCTGGCT GGTGGTGCGG GCGTTTTCCA TCGGCAAGCA 951 GTCGCGCGAT TTGGGTTTGA CTTTCAACGC CTATATCGCT TCGGGTATCG 1001 GCATTTGGAT CGGTATCCAA AGTTTCTTCA ATATCGGTGT GAACATCGGT 1051 GCTTTGCCGA CCAAAGGTCT GACGCTGCCG TTGATGTCCT ATGGCGGTTC 1101 GTCAGTCTTT TTCATGCTGA TCAGCATGAT GCTGCTGTTG CGTATAGATT 1151 ATGAAAACCG CCGGAAAATG CGCGGTTACC GGGTGGAGTA A This corresponds to the amino acid sequence <SEQ ID 276; ORF 086.a>: a086.pep 1 MWLMTAFSL LMIYSASVYL ASKEGGDQFF YLTRQAGFW AGLIASGLLW 51 FLCRMRTWRR LVPWIFALSG LLLVWLIAG REIΝGATRWI PLGPLΝFQPT 101 ELFKLAVILY LASLFTRREE VLRSMESLGW QSIWRGTAΝL IMSATΝPQAR 151 RETLEMYGRF RAIILPIMLV AFGLVLIMVQ PDFGSFWIT VIAVGMLFLA 201 GLPWKYFFVL VGSVLGGMVL MITAAPYRVQ RWAFLDPWK DPQGAGYQLT 251 HSLMAIGRGE WFGMGLGASL SKRGFLPEAH TDFIFAIIAE EFGFFGMCVL 301 IFCYGWLWR AFSIGKQSRD LGLTFNAYIA SGIGIWIGIQ SFFNIGVNIG 351 ALPTKGLTLP LMSYGGSSVF FMLISMMLLL RIDYENRRKM RGYRVE* m086/a086 98.0%> identity over a 396 aa overlap 10 20 30 40 50 60 m086 . pep MVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFWAGLIASGLLWFLCRMRTWRR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I aO 86 MWLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFWAGLIASGLLWFLCRMRTWRR 10 20 30 40 50 60 70 80 90 100 110 120 m086.pep LVPWIFALSGLLLVWLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLFTRREE I II I I II II I II I II II II II I I I I II II I II II II I I II I II I M I M I M I II I I II I a086 LVPWIFALSGLLLVWLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLFTRREE 70 80 90 100 110 120 130 140 150 160 170 180 m086.pep VLRSMESLGWQSIWRGTANLIMSATNPQXRRETLEMYGRXRAIILPIMLVAFGLVLIMVQ I II I II I I I II I I I I I II II II II I II I I II II I I I II II I I II II I I II II I II II I a086 VLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFGLVLIMVQ 130 140 150 160 170 180 190 200 210 220 230 240 m086.pep PDFGSFWITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQRVVAFLDPWK I I I I II II II II II I II II II I II I II I II II II I I I I II II II II II II II I I II II I I a086 PDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQRWAFLDPWK 190 200 210 220 230 240 250 260 270 280 290 300 m086.pep DPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIAEEFGFFGMCVL I II II II I I II I I I II I I II I II II I I II II II I II I II II II II II II I II II I II II I a086 DPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIAEEFGFFGMCVL 250 260 270 280 290 300 310 320 330 340 350 360 m086.pep IFCYGWLWRAFSIGKQSRDLGLTFNAYIASGIGIWIGXQSFFNIGVNIGALPXKGLTXP I II I II I I II II I II II II I II II I I II II II II I II I I I II II I I I II II I M II I I a086 IFCYGWLWRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGVNIGALPTKGLTLP 310 320 330 340 350 360 370 380 390 m086.pep XMSXGGSSVFFMLISMMLLXRIDYENRRKMRGYRVEX II II I II I II II I II II I I II I II I I II II II I I a086 LMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVEX 370 380 390 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 277>: g087.seq 1 ATGGGCGGTA AAACCTTTAT GCTGATGGCG GGCGGAACGG GCGGACACAT 51 TTTCCCAGCT CTGGCTGTGG CGGATTCATT GCGCGTGCGC GGTCATCATG 101 TAATTTGGCT GGGCAGCAAG GATTCGATGG AAGAGCGCAT CGTGCCGCAA 151 TACGGCATAC GCTTGGAAAC GCTGGCGATT AAAGGAATAC GCGGCAACGG 201 CATCAAACGC AAGCTGATGC TTCCGTTTAC TCTGTACAAA ACCGTCCGCG 251 AAGCGCAGCG GATTATCCGC AAACACCGTG TCGAGTGCGT CATCGGCTTC 301 GGCGGTTTTG TTACCTTTCC CGGCGGTCTG GCGGCGAAAC TCTTGGGCGT 351 GCCGATTGTG ATTCACGAGC AAAACGCCGT GGCAGGCTTG TCCAACCGCC 401 AccTGTCGCg ctGGGCGAAA CGGGTGTTGT ACGCTTTTCC GAAAGCGTTC 451 AGCCACGAAG GCGGTTTGGT CGGCAACCCC GTCCGCGCCG ATATTAGCAA 501 CCTGCCCGTG CCTGCCGAAC GCTTCCAAGG GCGCGAAGGC CGTCTGAAAA 551 TTTTGGTGGT CGGCGGCAGT TTGGGTGCGG ACGTTTTGAA CAAAACCGTA 601 CCGCAGGCGT TGGCACTGCT GCCTGAAGAG GTGCGCCCGC AGATGTACCA 651 CCAGTCGGGG CGTAACAAGC TGGGCAATCT TCAGGCGGAT TATGACGCGT 701 TGGGCGTGAA AGCGGAATGC GTGGAATTTA TTACCGACAT GGTGTCCGCC 751 TACCGTGATG CCGATTTGGT GATTTGCCGT GCCGGCGCGC TGACGATTGC 801 CGAGTTGACG GCGGCGGGGC TGGGCGCGTT GTTAGTGCCG TATCCTCACG 851 CCGTTGATGA CCATCAAACC GCCAACGCGC GTTTCATGGT GCAGGCAGAA 901 GCGGGGCTGC TGTTGCCGCA AACCCAGTTG ACGGCGGAAA AACTCGCCGA 951 AATCCTCGGC AGCCTCAACC GCGAAAAATG CCTCAAATGG GCGGAAAACG 1001 CCCGTACGTT GGCATTGCCG CACAGCGCGG ATGACGTTGC CGAAGCCGCG 1051 ATTGCGTGTG CGGCGTAAA This corresponds to the amino acid sequence <SEQ ID 278; ORF 087.ng>: g087.pep 1 MGGKTFMLMA GGTGGHIFPA LAVADSLRVR GHHVIWLGSK DSMEERIVPQ 51 YGIRLETLAI KGIRGΝGIKR KLMLPFTLYK TVREAQRIIR KHRVECVIGF 101 GGFVTFPGGL AAKLLGVPIV IHEQΝAVAGL SΝRHLSRWAK RVLYAFPKAF 151 SHEGGLVGΝP VRADISΝLPV PAERFQGREG RLKILWGGS LGADVLΝKTV 201 PQALALLPEE VRPQMYHQSG RNKLGNLQAD YDALGVKAEC VEFITDMVSA 251 YRDADLVICR AGALTIAELT AAGLGALLVP YPHAVDDHQT ANARFMVQAE 301 AGLLLPQTQL TAEKLAEILG SLNREKCLKW AENARTLALP HSADDVAEAA 351 IACAA* The following partial DNA sequence was identified in N meningitidis <SEQ ID 279>: m087.seq 1 ATGGGCGGTA AAACCTTTAT GCTGAwkkCG GGCGGAACGG GCGGACATAT 51 TTTCCCCGCG CTGGCGGTGG CGGATTCATT GCGCGCGCGC GGCCATCATG 101 TGATTTGGCT GGGCAGCAAG GATTCGATGG AAGAGCGTAT CGTGCCGCAA 151 TACGGCATAC GCTTGGAAAC GCTGGCGATT AAAGGCGTGC GCGGCAACGG 201 CATCAAACGC AAACTGATGC TGCCGGTTAC TTTGTATCAA ACCGTCCGCG 251 AAGCGCAGCG GATTATCCGC AAACACCGTG TCGAGTGCGT CATCGGCTTC 301 GGCGGCTTCG TTACCTTCCC CGGCGGTTTG GCGGCGAAGC TATTArGCGT 351 GCCGATTGTG ATTCACGAGC AAAACGCCGT GGCAGGTTTG TCCAACCGCC 401 ACCTGTCGCG CTGGGCGAAG CGGGTGTTGT ACGCTTTTCC GAAAGCGTTC 451 AGCCACGAAG GCGGCTTGGT CGGCAACCCC GTCCGCGCCG ATATTAGCAA 501 CCTGCCCGTG CCTGCCGAAC GCTTCCAAGG GCGTGAAGGC CGTCTGAAAA 551 TTTTGGTGGT CGGCGGCAGT TTGGGCGCGG ACGTTTTGAA CAAAACCGTA 601 CCGCATGCAT TGGCTTTGCT GCCCGACAAT GCGCGTCCGC ATATGTACCA 651 CCAATCGGGA CGGGGCAAGC TGGGCATCTT GCAGGCGnnn nnnnnnnnnn 701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 751 nnnGCGGGAT TGGGTGCGTT GTTAGTGCCG TATCCTCACG CGGTTGACGA 801 TCACCAAACC GCCAACGCGC GTTTTATGGT GCAGGCGGAG GCGGGATTGC 851 TGTTGCCGCA AACCCAGTTG ACGGCGGAAA AACTCGCCGA GATTCTCGGC 901 GGCTTAAACC GCGAAAAATG CCTCAAATGG GCAGAAAACG CCCGTACGTT 951 GGCACTGCCG CACAGTGCGG ACGACGTGGC GGAAGCCGCG ATTGCGTGTG 1001 CGGCGTAA This corresponds to the amino acid sequence <SEQ ID 280; ORF 087>: m087.pep 1 MGGKTFMLXX GGTGGHIFPA LAVADSLRAR GHHVIWLGSK DSMEERIVPQ 51 YGIRLETLAI KGVRGΝGIKR KLMLPVTLYQ TVREAQRIIR KHRVECVIGF 101 GGFVTFPGGL AAKLLXVPIV IHEQΝAVAGL SΝRHLSRWAK RVLYAFPKAF 151 SHEGGLVGΝP VRADISΝLPV PAERFQGREG RLKILWGGS LGADVLΝKTV 201 PHALALLPDΝ ARPHMYHQSG RGKLGILQAX XXXXXXXXXX XXXXXXXXXX 251 XAGLGALLVP YPHAVDDHQT ANARFMVQAE AGLLLPQTQL TAEKLAEILG 301 GLNREKCLKW AENARTLALP HSADDVAEAA IACAA* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 087 shows 83.9% identity over a 355 aa overlap with a predicted ORF (ORF 087.ng) from N. gonorrhoeae: m087/g087 10 20 30 40 50 60 m087.pep MGGKTFMLXXGGTGGHIFPALAVADSLRARGHHVIWLGSKDSMEERIVPQYGIRLETLAI MMMMMMMMMMMMMMMMMMMMMMMMM g087 MGGKTFMLMAGGTGGHIFPALAVADSLRVRGHHVIWLGSKDSMEERIVPQYGIRLETLAI 10 20 30 40 50 60 70 80 90 100 110 120 mO 87. pep KGVRGΝGIKRKLMLPVTLYQTVREAQRI IRKHRVECVIGFGGFVTFPGGLAAKLLXVPIV I h l l l l l l l l l l l l M I M M M M M M M M M M M M M M M M M M M M g087 KGIRGΝGIKRKLMLPFTLYKTVREAQRIIRKHRVECVIGFGGFVTFPGGLAAKLLGVPIV 70 80 90 100 110 120 130 140 150 160 170 180 m087.pep IHE Q ΝAVAGLSΝRHLSRWAKRVLYAFPKAFSHEGGLVGΝPVRADISΝLPVPAERFQGREG MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g087 IHE Q ΝAVAGLSΝRHLSRWAKRVLYAFPKAFSHEGGLVGΝPVRADISΝLPVPAERFQGREG 130 140 150 160 170 180 190 200 210 220 229 m087.pep RLKILWGGSLGADVLNKTVPHALALLPDNARPHMYHQSGRGKLGILQA- g087 RLKILWGGSLGADVLNKTVPQALALLPEEVRPQMYHQSGRNKLGNLQADYDALGVKAEC 190 200 210 220 230 240 230 240 250 mO87. pep AGLGALLVPYPHAVDDHQTANARFMVQAE MMMMMMMMMMMIMMM g087 VEFITDMVSAYRDADLVICRAGALTIAELTAAGLGALLVPYPHAVDDHQTANARFMVQAE 250 260 270 280 290 300 260 270 280 290 300 310 mO87. ep AGLLLPQTQLTAEKLAEILGGLNREKCLKWAENARTLALPHSADDVAEAAIACAAX MMMMMMMMMIhMMM MMMMMMMMMMMIMMM gO 87 AGLLLPQTQLTAEKLAEILGSLNREKCLKWAENARTLALPHSADDVAEAAIACAAX 310 320 330 340 350 The following partial DNA sequence was identified in N meningitidis <SEQ ID 281>: a087.seq 1 ATGGGCGGTA AAACCTTTAT GCTGATGGCG GGCGGAACGG GCGGACATAT 51 TTTCCCCGCG CTGGCGGTGG CGGATTCATT GCGCGCGCGC GGCCATCATG 101 TAATTTGGCT GGGCAGCAAG GATTCGATGG AAGAGCGCAT CGTGCCGCAA 151 TACGACATCC TGCTCGAAAC GCTGGCGATT AAAGGCGTGC GCGGCAACGG 201 CATCAAACGC AAGCTGATGC TGCCGTTTAC TTTGTATCAA ACTGTCCGCG 251 AAGCGCAGCA GATTATCCGC AAACACCGTG TCGAGTGCGT CATCGGCTTC 301 GGCGGCTTCG TTACCTTTCC CGGCGGTTTG GCGGCGAAGT TATTAGGCGT 351 GCCGATTGTG ATTCACGAGC AAAACGCCGT GGCAGGTTTG TCCAACCGCC 401 ACCTGTCGCG CTGGGCGAAG CGGGTGTTGT ACGCTTTTCC GAAAGCGTTC 451 AGCCACGAAG GCGGCTTGGT CGGCAACCCC GTCCGCGCCG ATATTAGCAA 501 CCTGCCCGTG CCTGCCGAAC GCTTCCAAGG GCGTGAAGGC CGTCTGAAAA 551 TTTTGGTGGT CGGCGGCAGT TTGGGCGCGG ACGTTTTGAA CAAAACCGTA 601 CCGCAGGCAT TGGCTTTGCT GCCCGACAAT GCGCGTCCGC AGATGTACCA 651 CCAATCGGGA CGGGGCAAGC TGGGCAGCTT GCAGGCGGAT TACGACGCGC 701 TGGGCGTGCA AGCGGAATGC GTGGAATTTA TTACCGATAT GGTGTCCGCC 751 TACCGCGATG CCGATTTGGT GATTTGCCGT GCCGGCGCGC TGACGATTGC 801 CGAGTTGACG GCGGCGGGAT TGGGTGCGTT GTTAGTGCCG TATCCTCACG 851 CCGTTGATGA CCATCAAACC GCCAACGCGC GTTTTATGGT GCAGGCGGAG 901 GCGGGATTGC TGTTGCCGCA AACCCAGTTG ACGGCGGAAA AACTCGCCGA 951 GATTCTCGGC GGCTTAAACC GCGAAAAATG CCTCAAATGG GCAGAAAACG 1001 CCCGTACGTT GGCACTGCCG CACAGTGCGG ACGACGTTGC CGAAGCCGCG 1051 ATTGCGTGTG CGGCGTAA This corresponds to the amino acid sequence <SEQ ID 282; ORF 087. a>: a087.pep 1 MGGKTFMLMA GGTGGHIFPA LAVADSLRAR GHHVIWLGSK DSMEERIVPQ 51 YDILLETLAI KGVRGΝGIKR KLMLPFTLYQ TVREAQQIIR KHRVECVIGF 101 GGFVTFPGGL AAKLLGVPIV IHEQΝAVAGL SNRHLSRWAK RVLYAFPKAF 151 SHEGGLVGΝP VRADISΝLPV PAERFQGREG RLKILWGGS LGADVLNKTV 201 PQALALLPDΝ ARPQMYHQSG RGKLGSLQAD YDALGVQAEC VEFITDMVSA 251 YRDADLVICR AGALTIAELT AAGLGALLVP YPHAVDDHQT ANARFMVQAE 301 AGLLLPQTQL TAEKLAEILG GLNREKCLKW AENARTLALP HSADDVAEAA 351 IACAA* m087/a087 85.4% identity over a 355 aa overlap 10 20 30 40 50 60 m087.pep MGGKTFMLXXGGTGGHIFPALAVADSLRARGHHVIWLGSKDSMEERIVPQYGIRLETLAI llllllll II II II I I II II II I II II II II I II I I II II II I II I II I I llllll a087 MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSMEERIVPQYDILLETLAI 10 20 30 40 50 60 70 80 90 100 110 120 m087. pep KGVRGNGIKRKLMLPVTLYQTVREAQRIIRKHRVECVIGFGGFVTFPGGLAAKLLXVPIV I I II I II II II I I II II II II I II I : II II II I II II I II I II II I II I II I I I II II a087 KGVRGNGIKRKLMLPFTLYQTVREAQQIIRKHRVECVIGFGGFVTFPGGLAAKLLGVPIV 70 80 90 100 110 120 130 140 150 160 170 180 m087. pep IHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNPVRADISNLPVPAERFQGREG II I I I I II II I I I II II II I II I II I II I II I I II I II II I I II II I II II I I I I II I II a087 IHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNPVRADISNLPVPAERFQGREG 130 140 150 160 170 180 190 200 210 220 230 240 m087. pep RLKILWGGSLGADVLNKTVPHALALLPDNARPHMYHQSGRGKLGILQAXXXXXXXXXXX II I II II I I II II I II II II I : I II I II I II II : II II I II I I II III a087 RLKILWGGSLGADVLNKTVPQALALLPDNARPQMYHQSGRGKLGSLQADYDALGVQAEC 190 200 210 220 230 240 250 260 270 280 m087.pep XX XXXXXXXXXAGLGALLVPYPHAVDDHQTANARFMVQAE : : I II II I II I I II II II II I I II II I II II a087 VEFITDMVSAYRDADLVICRAGALTIAELTAAGLGALLVPYPHAVDDHQTANARFMVQAE 250 260 270 280 290 300 290 300 310 320 330 m087. pep AGLLLPQTQLTAEKLAEILGGLNREKCLKWAENARTLALPHSADDVAEAAIACAAX II II I II II I I I I I II II II II I II I I II II II I II I II I II I II I I II I II I I II a087 AGLLLPQTQLTAEKLAEILGGLNREKCLKWAENARTLALPHSADDVAEAAIACAAX 310 320 330 340 350 The following partial DNA sequence was identified in N.gonorrhoeae <SEQ ID 283>: g088.seq 1 ATGTTTTTAT GGCTCGCACA TTTCAGCAAC TGGTTAACCG GTCTGAATAT 51 TTTTCAATAC ACCACATTCC GCGCCGTTAT GGCGGCGTTG ACCGCCTTGG 101 CGTTTTCCCT GATGTTCGGC CCGTGGACGA TACGCAGGCT GACCGCGCTC 151 AAATGCGGGC AGGCAGTGCG TACCGACGGC CCGCAAACCC ACCTCGTCAA 201 AAACGGCACG CCGACGATGG GCGGTTCGCT GATTCTGACC GCCATTACCG 251 TGTCCACCCT GTTGTGGGGC AACTGGGCGA ACCCGTATAT CTGGATTCTC 301 TTGGGCGTAC TGCTTGCCAC CGGTGCGCTC GGTTTTTACG ACGACTGGCG 351 CAAAGTCGTT TATAAAGACC CCAACGGCGT GTCCGCCAAA TTCAAAATGG 401 TGTGGCAGTC AAGCGTTGCC GTTatcgcCG GTttggcaTT GTTTTACctt 451 gCcgcCAATT CCGCCAACAA TATTTTGATT GTCCCGtttT TCAAACAAAT 501 CGCCCTGCCG CTGGGCGTGG TCGGCTTttt gGtgttgTCT TACCTGACCA 551 TCGTCGGCAC ATCCAACGCC GTCAACCTCA CcgaCGGCTT GGACGGCCTT 601 GCCGCcttcc cgttcgtcct cgttgccgcC GGGCTCGCCA ttttcgccTA 651 CGTCAGCGGA CACTACCAAT TTTCCCAATA CCTCCAGCTT CCCTATGTCG 701 CCGGCGCGAA CGAAGTCGCT ATATTCTGCA CCGCCATGTG CGGCGCGTGC 751 CTCGGATTTT TGTGGTTCAA CGCCTATCCC GCGCAAGTCT TTATGGGCGA 801 TGTCGGCGCG CTGGCATTGG GTGCCGCGCT CGGTaccGtt gCCGTcaTcg 851 tCCGCCAAGA ATTTGTcctc gtcattaTGG GCGGTCTGTT cgtcgtagaa 901 gccgtgTCCG TTATGCTTCa tgtcggCTGG TACAAGAAAA Ccaaaaaacg 951 CATCTTcCTg acgGcaccga ttcatcacca ttaCCaactt cgatgCTGGa 1001 aagaaacgca agtcgtcgtc CGTTtCTGGA TTAtTAccat cgtcgtggtt 1051 tTgataggtt tGagtacccT caAAattcgc ggaaactatg ccgTCCGAAC 1101 ACCTTTCAGA CGGCATTTGA ACGCGCAATA A This corresponds to the amino acid sequence <SEQ ID 284; ORF 088.ng>: g088.pep 1 MFLWLAHFSN WLTGLNIFQY TTFRAVMAAL TALAFSLMFG PWTIRRLTAL 51 KCGQAVRTDG PQTHLVKNGT PTMGGSLILT AITVSTLLWG NWANPYIWIL 101 LGVLLATGAL GFYDDWRKW YKDPNGVSAK FKMVWQSSVA VIAGLALFYL 151 AANSANNILI VPFFKQIALP LGWGFLVLS YLTIVGTSNA VNLTDGLDGL 201 AAFPFVLVAA GLAIFAYVSG HYQFSQYLQL PYVAGANEVA IFCTAMCGAC 251 LGFLWFNAYP AQVFMGDVGA LALGAALGTV AVIVRQEFVL VIMGGLFWE 301 AVSVMLHVGW YKKTKKRIFL TAPIHHHYQL RCWKETQVW RFWIITIWV 351 LIGLSTLKIR GNYAVRTPFR RHLNAQ* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 285>: m088. seq 1 ATGTTTTTAT GGCTCGCACA TTTCAGCAnC TGGTTAACCG GTCTGAATnn 51 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 151 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 251 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 351 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 451 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 501 nnnnnnnnnn nnnGGCGTGG TCGGCTTTTT GGTGTTGTCT TACCTGACCA 551 TCGTCGGCAC ATCCAATGCC GTCAACCTCA CCGACGGCTT GGACGGCCTT 601 GCGACCTTCC CCGTCGTCCT CGTTGCCGCC GGCCTCGCCA TCTTCGCCTA 651 TGCCAGCGGC CACTCACAAT TTGCCCAATA CCTGCAATTA CCTTACGTTG 701 CCGGCGCAAA CGAAGTGGTG ATTTTCTGTA CCGCCATGTG CGGCGCGTGC 751 CTCGGTTTCT TGTGGTTTAA CGCCTATCCC GCGCAAGTCT TTATGGGCGA 801 TGTCGGTGCA TTGGCATTGG GTGCCGCGCT CGGTACCGTC GCCGTTATCG 851 TCCGCCAAGA GTTTGTCCTC GTCATTATGG GCGGATTATT TGTCGTAGAA 901 GCCGTATCCG TTATGCTTCA GGTTGGCTGG TATAAGAAAA CCAAAAAACG 951 CATCTTCCTG ATGGCGCCCA TCCATCACCA CTACGAACAA AAAGGCTGGA 1001 AAGAAACCCA AGTCGTCGTC CGCTTTTGGA TTATTACCAT CGTCTTGGTG 1051 TTGATCGGTT TGAGTACCCT CAAAATCCGC TGAACCTATG CCGTCTGAAC 1101 ATCTTTCAGA CGGCATTTGA ACGCGCAATA A 1 MFLWLAHFSN WLTGLNIFQY TTFRAVMAAL TALAFSLMFG PWTIRRLTAL 51 KCGQAVRTDG PQTHLVKNGT PTMGGSLILT AITVSTLLWG NWANPYIWIL 101 LGVLLATGAL GFYDDWRKW YKDPNGVSAK FKMVWQSSVA VIAGLALFYL 151 AANSANNI I VPFFKQIALP LG GFLVLS YLTIVGTSNA VNLTDGLDGL 201 AAFPFVLVAA GLAIFAYVSG HYQFSQYLQL PYVAGANEVA IFCTAMCGAC 251 LGFLWFNAYP AQVFMGDVGA LALGAALGTV AVIVRQEFVL VIMGGLFWE 301 AVSVMLHVGW YKKTKKRIFL TAPIHHHYQL RCWKETQVW RFWIITIWV 351 LIGLSTLKIR GNYAVRTPFR RHLNAQ* This corresponds to the amino acid sequence <SEQ ID 286; ORF 088>: m088.pep 1 MFLWLAHFSX WLTGLNXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX 51 XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX 101 XXXXXXXXXX XXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX 151 XXXXXXXXXX XXXXXXXXXX XGWGFLVLS YLTIVGTSNA VNLTDGLDGL 201 ATFPWLVAA GLAIFAYASG HSQFAQYLQL PYVAGANEW IFCTAMCGAC 251 LGFLWFNAYP AQVFMGDVGA LALGAALGTV AVIVRQEFVL VIMGGLFWE 301 AVSVMLQVGW YKKTKKRIFL MAPIHHHYEQ KGWKETQVW RFWIITIVLV 351 LIGLSTLKIR XTYAVXTSFR RHLNAQ* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 088 shows 91.7% identity over a 205 aa overlap with a predicted ORF (ORF 088.ng) from N gonorrhoeae: m088/g088 10 20 30 mO 88 . pep G GFLVLSYLTIVGTSΝAVΝLTDGLDGLA 1 1 M 1 1 1 1 I I 1 1 1 1 1 1 M 1 1 1 I 1 1 1 1 1 1 M g088 IAGLALFYLAAΝSAΝΝILIVPFFKQIALPLGWGFLVLSYLTIVGTSΝAVΝLTDGLDGLA 150 160 170 180 190 200 40 50 60 70 80 90 mO88. pep TFPWLVAAGLAIFAYASGHSQFAQYLQLPYVAGANE IFCTAMCGACLGFLWFNAYPA Ml MMMMMMMM I M II II I II 11111 II M 11 II 111 II II I II 11 II II gO88 AFPFVLVAAGLAIFAYVSGHYQFSQYLQLPYVAGANEVAIFCTAMCGACLGFLWFNAYPA 210 220 230 240 250 260 100 110 120 130 140 150 mO 88. pep QVFMGDVGALALGAALGTVAVIVRQEFVLVIMGGLFWEAVSVMLQVGWYKKTKKRIFLM MMMMMMMMIMMMMIIMMMIMMMMMIMMMMMMM gO 88 QVFMGDVGALALGAALGTVAVIVRQEFVLVIMGGLFWEAVSVMLHVGWYKKTKKRIFLT 270 280 290 300 310 320 160 170 180 190 200 m088.pep APIHHHYEQKGWKETQVWRFWIITIVLVLIGLSTLKIRXTYAVXTSFRRHLNAQX : MMMMMMMMMMMMMM MM I M l gO 88 APIHHHYQLRCWKETQWVRFWIITIVWLIGLSTLKIRGNYAVRTPFRRHLNAQX 330 340 350 360 370 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 287>: a088.seq 1 ATGTTTTTAT GGCTCGCACA TTTCAGCAAC TGGTTAACCG GTCTGAATAT 51 TTTTCAATAC ACCACATTCC GCGCCGTCAT GGCGGCGTTG ACCGCCTTGG 101 CGTTTTCCCT GATGTTCGGC CCGTGGACGA TACGCAGGCT GACCGCGCTC 151 AAATGCGGGC AGGCAGTGCG TACCGACGGT CCGCAAACCC ACCTCGTCAA 201 AAACGGCACG CCGACGATGG GCGGTTCGCT GATTCTGACC GCCATTACCG 251 TGTCCACCCT GTTGTGGGGC AACTGGGCAA ACCCGTATAT CTGGATTCTC 301 TTGGGCGTAT TGCTCGCCAC GGGCGCACTC GGTTTTTACG ACGACTGGCG 351 CAAAGTCGTC TATAAAGACC CCAACGGCGT GTCCGCCAAA TTCAAAATGG 401 TGTGGCAGTC AAGCGTTGCC ATTATCGCCG GTTTGGCATT GTTTTACCTT 451 GCCGCCAATT CCGCCAACAA TATTTTGATT GTCCCGTTCT TCAAACAAAT 501 CGCCCTGCCG CTGGGCGTGG TCGGCTTTTT GGTGTTGTCT TACCTGACCA 551 TCGTCGGCAC ATCCAATGCC GTCAACCTCA CCGACGGCTT GGACGGCCTT 601 GCGACCTTCC CCGTCGTCCT CGTTGCCGCC GGCCTCGCCA TCTTCGCCTA 651 TGCCAGCGGC CACTCACAAT TTGCCCAATA CCTGCAATTA CCTTACGTTG 701 CCGGCGCAAA CGAAGTGGTG ATTTTCTGTA CCGCCATGTG CGGCGCGTGC 751 CTCGGTTTCT TGTGGTTTAA CGCCTATCCC GCGCAAGTCT TTATGGGCGA 801 TGTCGGTGCA TTGGCATTGG GTGCCGCGCT CGGTACCGTC GCCGTCATCG 851 TCCGCCAAGA GTTTGTCCTC GTCATTATGG GCGGATTATT TGTCGTAGAA 901 GCCGTATCCG TTATGCTTCA GGTCGGCTGG TATAAGAAAA CCAAAAAACG 951 CATCTTCCTG ATGGCGCCCA TCCATCACCA CTACGAACAA AAAGGCTGGA 1001 AAGAAACCCA AGTCGTCGTC CGCTTTTGGA TTATTACCAT CGTCTTGGTG 1051 TTGATCGGTT TGAGTACCCT CAAAATCCGC TGAACCTATG CCGTCTGAAC 1101 ACCTTTCAGA CGGCATTTGA ACGCGCAATA A This corresponds to the amino acid sequence <SEQ ID 288; ORF 088. a>: a088.pep 1 MFLWLAHFΞΝ WLTGLΝIFQY TTFRAVMAAL TALAFSLMFG PWTIRRLTAL 51 KCGQAVRTDG PQTHLVKΝGT PTMGGSLILT AITVSTLLWG ΝWAΝPYIWIL 101 LGVLLATGAL GFYDDWRKVV YKDPΝGVSAK FKMVWQSSVA IIAGLALFYL 151 AAΝSAΝΝILI VPFFKQIALP LGWGFLVLS YLTIVGTSNA VNLTDGLDGL 201 ATFPWLVAA GLAIFAYASG HSQFAQYLQL PYVAGANEW IFCTAMCGAC 251 LGFLWFNAYP AQVFMGDVGA LALGAALGTV AVIVRQEFVL VIMGGLFWE 301 AVSVMLQVGW YKKTKKRIFL MAPIHHHYEQ KGWKETQWV RFWIITIVLV 351 LIGLSTLKIR *TYAV*TPFR RHLNAQ* m088/a088 99.5% identity over a 205 aa overlap 150 160 170 180 190 200 m088. pep XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGWGFLVLSYLTIVGTSNAVNLTDGLDGLA I M I II II I II I II I II II II I II II II I I a088 IAGLALFYLAANSANNILIVPFFKQIALPLGWGFLVLSYLTIVGTSNAVNLTDGLDGLA 150 160 170 180 190 200 210 220 230 240 250 260 m088. pep TFPWLVAAGLAIFAYASGHSQFAQYLQLPYVAGANEWIFCTAMCGACLGFLWFNAYPA I I I I II I I I I II I II II I II I II II II I II I II I II II II II II I I II II II II I I II II aO88 TFPWLVAAGLAIFAYASGHSQFAQYLQLPYVAGANEWIFCTAMCGACLGFLWFNAYPA 210 220 230 240 250 260 270 280 290 300 310 320 m088. pep QVFMGDVGALALGAALGTVAVIVRQEFVLVIMGGLFWEAVSVMLQVGWYKKTKKRIFLM I I I I II II I II I I II I II I I I II I I I II II II I II I I I I I II II I II II II II I I II I II a088 QVFMGDVGALALGAALGTVAVIVRQEFVLVIMGGLFWEAVSVMLQVGWYKKTKKRIFLM 270 280 290 300 310 320 330 340 350 360 370 m088. pep APIHHHYEQKGWKETQVWRFWIITIVLVLIGLSTLKIRXTYAVXTSFRRHLNAQX II I II I I II I II II II I II I II I II I II I II I II II I II II II II I I I I II I II I a088 APIHHHYEQKGWKETQVWRFWIITIVLVLIGLSTLKIRXTYAVXTPFRRHLNAQX 330 340 350 360 370 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 289>: g089.seq 1 ATGCCGCCCA AAATCACGAA GAGCGGGTTT TGCAAACCGG CAATCGCGGC 51 GGCGGTCGCG CCGACATTCG TGCCTTTGCT GTCGTCGATG AATACCACGC 101 CGTTTTTCTC GCCGATTTTT TCCACACGGT GCGGCAAGCC TTGGAAGGTT 151 TTGACGTGTT CCAGCAATGC TTCGCGCGGC AAACCGACGG CCTCGCACAA 201 AGCCACGGCA GCCATAACGT TGGCGGCGTT GTGCAAACCT TGCAGCGGGA 251 TGTCTTGCGT AGAAATCAAA TCTTCATTGC CTTGTTTTAA ACAGCCCGTC 301 CCGCGTTCCA ACCAAAAATC GGCTTCGTGT TCCAAGGAAA ACCGTTTCAC 351 TTCACGCCCT GCCCGTTTCA TGGCGCGGCA GAACACGTCG TCCGCATTCA 401 AAACCTGCAC TCCATCGCCA CGGAAAATCT CGGCTTTGGT ATGCGCGTAG This corresponds to the amino acid sequence <SEQ ID 290; ORF 089.ng>: g089.pep 1 MPPKITKSGF CKPAIAAAVA PTFVPLLSSM ΝTTPFFSPIF STRCGKPWKV 51 LTCSSΝASRG KPTASHKATA AITLAALCKP CSGMSCVEIK SSLPCFKQPV 101 PRSΝQKSASC SKEΝRFTSRP ARFMARQΝTS SAFKTCTPSP RKISALVCA* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 291>: m089. seq 1 ATGCCGCCCA AAATCACkA GAGCGGATTT TGCAAACCGG CAATCGCGGC 51 GGCAGTCGCG CCGACATTCG TGCCTTTGCT GTCGTCGATA AACACCACGC 101 CGTTTTTCTC GCCGATTTTT TCCACGCGGT GCGGCAGGCC TTGGAAGGTT 151 TTGACGTGTT CGAGCAATGC TTCGCGCGAC AAACCGATGG CCTCACACAA 201 AGCCACkGCA GCCATGACGT TAGCGGCGTT GTGCAkACCT TGCAACGG A 251 TGTCTTGCGT GACAATCAAA TCTTCATTGC CTTGTTTCAG GCGGCCTGTC 301 TCGCGTTCCA ACCAGAAATC AGCTTCGTGT TCCAACGAAA ACCATTTTAC 351 CTCGCGCCCG GCACGCTTCA TCGCGCGGCA GAACGCATCG TCCGCATTCA 401 AAACCTGCAC GCCGTCGCCA CGGAAAATCT TGGCTTTGGT ATGCGCATAG This corresponds to the amino acid sequence <SEQ ID 292; ORF 089>: m089.pep 1 MPPKITXSGF CKPAIAAAVA PTFVPLLSSI ΝTTPFFSPIF STRCGRPWKV 51 LTCSSΝASRD KPMASHKATA AMTLAALCXP CΝGMSCVTIK SSLPCFRRPV 101 SRSΝQKSASC SΝEΝHFTSRP ARFIARQΝAS SAFKTCTPSP RKILALVCA* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N. gonorrhoeae ORF 089 shows 88.6%> identity over a 149 aa overlap with a predicted ORF (ORF 089.ng) from N gonorrhoeae: m089/g089 10 20 30 40 50 60 m089.pep MPPKITXSGFCKPAIAAAVAPTFVPLLSSINTTPFFSPIFSTRCGRPWKVLTCSSNASRD MMMMMMMMMMMMMMMMMMMMMMMMMM g089 MPPKITKSGFCKPAIAAAVAPTFVPLLSSMNTTPFFSPIFSTRCGKPWKVLTCSSNASRG 10 20 30 40 50 60 70 80 90 100 110 120 m089.pep KPMASHKATAAMTLAALCXPCNGMSCVTIKSSLPCFRRPVSRSNQKSASCSNENHFTSRP I I M M M M M I M M I I M I I M M M M I h M I I I I I I I I 1 1 I M I M 1 1 I I g089 KPTASHKATAAITLAALCKPCSGMSCVEIKSSLPCFKQPVPRSNQKSASCSKENRFTSRP 70 80 90 100 110 120 130 140 150 m089.pep ARFIARQNASSAFKTCTPSPRKILALVCAX 11 hi ! I hi 1111111111111 g089 ARFMARQNTSSAFKTCTPSPRKISALVCAX 130 140 150 The following partial DNA sequence was identified in N meningitidis <SEQ ID 293>: a089.seq 1 ATGCCGCCTA AAATCACGAA GAGCGGATTT TGCAAACCGG CAATCGCGGC 51 GGCGGTCGCA CCGACGTTCG TGCCTTTGCT GTCGTCGATG AACACCACGC 101 CATTTTTCTC GCCGATTTTT TCCACGCGGT GCGGCAGGCC TTGAAAGGTT 151 TTGACGTGTT CGAGCAATGC TTCGCGCGGC AAACCGACGG CTTCGCACAA 201 GGCAACGGCA GCCATCACGT TAGTGGCGTT GTGCAAGCCT TGCAGCGGAA 251 TATCTTGCGT GGCAATCAAA TCTTCATTGC CTTGTTTCAG GCGACCTGTC 301 TCACGTTCCA ACCAAAAATC GGCTTCGTAT TCCAACGAAA ACCATTTCAC 351 CTCGCGCCCG GCGCGCTTCA TCGCACGACA GAACGCATCG TCCGCATTCA 401 AAACCTGCAC ACCGTCGCCA CGGAAAATCT TGGCTTTGGT ATGCGCGTAG This corresponds to the amino acid sequence <SEQ ID 294; ORF 089. a>: a089.pep 1 MPPKITKSGF CKPAIAAAVA PTFVPLLSSM ΝTTPFFSPIF STRCGRP*KV 51 LTCSSΝASRG KPTASHKATA AITLVALCKP CSGISCVAIK SSLPCFRRPV 101 SRSΝQKSASY SΝEΝHFTSRP ARFIARQΝAS SAFKTCTPSP RKILALVCA* m089/a089 91.9% identity over a 149 aa overlap 10 20 30 40 50 60 m089.pep MPPKITXSGFCKPAIAAAVAPTFVPLLSSIΝTTPFFSPIFSTRCGRPWKVLTCSSΝASRD llllll II I I II II II II II II II I I I I : I II II II II I I I I I II I II II II II I II a089 MPPKITKSGFCKPAIAAAVAPTFVPLLSSMΝTTPFFSPIFSTRCGRPXKVLTCSSΝASRG 10 20 30 40 50 60 70 80 90 100 110 120 m089.pep KPMASHKATAAMTLAALCXPCΝGMSCVTIKSSLPCFRRPVSRSΝQKSASCSΝEΝHFTSRP II I II I I II I : II : I II I I : I : II I : I I I I II I II II I I II I II II I I I II I I I II I a089 KPTASHKATAAITLVALCKPCSGISCVAIKSSLPCFRRPVSRSΝQKSASYSΝEΝHFTSRP 70 80 90 100 110 120 130 140 150 m089.pep ARFIARQNASSAFKTCTPSPRKILALVCAX II I II II II I I I II II II II II I II I II I I a089 ARFIARQNASSAFKTCTPSPRKILALVCAX 130 140 150 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 295>: g090. seq 1 ATGCGCGTAG TCGAGCAAAT CGTCGTAGCG GTCGAGATGG TCTTCGGAAA 51 TGTTCATCAC CGTCGCCGCA GTCGGGCGCA GGCTTTCGGT GTTTTCCAGT 101 TGGAAGCTGG AAAGCTCcca CACCCACACG TCCGCCTTTT TGCCTTCgcg 151 ctgCAATtct gcctccaaga cgggcgtacc gatATTGCCC GCAATGAcgg 201 tatccagccc gcacttgatg CAGAGatagc ggaccaggct ggttaccgTG 251 GTTttgccgt tgctgCcggt aatcgCaatc accttgtcgC CGCGGCGGtt 301 cAcaaTGTCc gccaGCAATt ggATGTCGCC TAgCACGCGC . ccgccgTTT 351 TGCttga This corresponds to the amino acid sequence <SEQ ID 296; ORF 090.ng>: g090.pep 1 MRWEQIWA VEMVFGNVHH RRRSRAQAFG VFQLEAGKLP HPHVRLFAFA 51 LQFCLQDGRT DIARNDGIQP ALDAEIADQA GYRGFAVAAG NRNHLVAAAV 101 HNVRQQLDVA XHAXRRFA* The following partial DNA sequence was identified in N meningitidis <SEQ ID 297>: m090. seq 1 ATGCGCATAG TCGAGCAAGT CGTCGTAGCG GTCGAGATGG TCTTCGGAAA 51 TGTTCAGCAC CGTCGCCGCA GTCGGACGCA GGCTTTCGGT GTTTTCCAGT 101 TGGAAGCTGG AAAGCTCCAA CACCCACACG TCCGCCTTTT TGCCTTCGCG 151 CTGCCATTCC GCCTCCAAAA CCGGCGTGCC GATATTGCCC GCGATAACGG 201 TATCCAGCCC GCACTTGATA CAGAGATAGC CGACCAGGCT CGTTACCGTG 251 GTTTTGCCGT TGCTGCCGGT AATCGCAATT ACCTTGTCGT CCCGGCGGTT 301 CACAATGTCC GCCAGCAATT CGATGTCGCC CAACACGCGT .CCGCCGTTT 351 TGCTTGA This corresponds to the amino acid sequence <SEQ ID 298; ORF 090>: m090.pep 1 MRIVEQVWA VEMVFGΝVQH RRRSRTQAFG VFQLEAGKLQ HPHVRLFAFA 51 LPFRLQΝRRA DIARDΝGIQP ALDTEIADQA RYRGFAVAAG ΝRΝYLWPAV 101 HΝVRQQFDVA QHAXRRFA* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N. gonorrhoeae ORF 090 shows 83.9% identity over a 118 aa overlap with a predicted ORF (ORF 090.ng) from N gonorrhoeae: m090/g090 10 20 30 40 50 60 mO90. pep MRIVEQVWAVEMVFGΝVQHRRRSRTQAFGVFQLEAGKLQHPHVRLFAFALPFRLQΝRRA M M M M M M M M M M M M I M M M M M M M l l l l l l l l l l l I I M h g090 MRWEQIWAVEMVFGΝVHHRRRSRAQAFGVFQLEAGKLPHPHVRLFAFALQFCLQDGRT 10 20 30 40 50 60 70 80 90 100 110 119 mO90. pep DIARDΝGIQPALDTEIADQARYRGFAVAAGΝRΝYLWPAVHΝVRQQFDVAQHAXRRFAX M l h M M M M M I M M M M M M M M M h 1 1 1 1 1 1 1 h 1 1 1 l l l l l l l l g090 DIARΝDGIQPALDAEIADQAGYRGFAVAAGΝRΝHLVAAAVHΝVRQQLDVAXHAXRRFAX 70 80 90 100 110 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 299>: aOΘO.seq 1 ATGCGCGTAG TCGAGCAAGT CGTCGTAGCG GTCGAGATGG TCTTCGGAAA 51 TGTTCAGCAC TGTCGCCGCA GTCGGGCGCA GGCTTTCGGT GTTTTCCAGT 101 TGGAAACTGG AAAGCTCCAA CACCCACACG TCCGCCTTTT TGCCTTCGCG 151 CTGCAATTCC GCCTCCAAAA CCGGCGCGCC GATATTGCCC GCGATAACGG 201 TATCCAGCCC ACACTTGATG CAGAGATAGC CGACCAGGCT CGTTACCGTG 251 GTTTTGCCGT TGCTGCCGGT AATCGCAATC ACCTTGTCGC CGCGGCGGTT 301 CACAATGTCC GCCAGCAATT CGATGTCGCC CAACACGCGT CCGCCGTTT 351 CGCTTAA This corresponds to the amino acid sequence <SEQ ID 300; ORF 090. a>: a090 . pep 1 MRWEQVWA VEMVFGNVQH CRRSRAQAFG VFQLETGKLQ HPHVRLFAFA 51 LQFRLQNRRA DIARDNGIQP TLDAEIADQA RYRGFAVAAG NRNHLVAAAV 101 HNVRQQFDVA QHAXRRFA* m09/a090 91.5%o identity over a 117 aa overlap 10 20 30 40 50 60 m090 . pep MRIVEQWVAVEMVFGNVQHRRRSRTQAFGVFQLEAGKLQHPHVRLFAFALPFRLQNRRA I I : I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I : I I I I I I I I I I I I I I I l l l l l l l l a090 MRWEQVWAVEMVFGNVQHCRRSRAQAFGVFQLETGKLQHPHVRLFAFALQFRLQNRRA 10 20 30 40 50 60 70 80 90 100 110 119 m090. pep DIARDNGIQPALDTEIADQARYRGFAVAAGNRNYLWPAVHNVRQQFDVAQHAXRRFAX II II II II II : II : I II II II I I II I II II I II : I I : II I II II I II I II I I II I I II a090 DIARDNGIQPTLDAEIADQARYRGFAVAAGNRNHLVAAAVHNVRQQFDVAQHAXRRFAX 70 80 90 100 110 The following partial DNA sequence was identified in N gonorrhoeae g090-l.seq This sequence contains multiple stop codons (not shown) This corresponds to the amino acid sequence < ORF 090-l.ng>: g090-l .pep (not shown) The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2>: m090-l.seq 1 ATGACGGCGT TTGCATTTCA GACGGCATCA CAAAGCCTTA AACGCTTCGA 51 TAAACACTTC CGAACGGTGC GCGTAGCCTT TGAACATATC AAAGCTCGCG 101 CAGGCGGGGC TGAGCAACAC AATATCGCCT GCTTCGGCTT GGGCATATGC 151 CGTCTGAACG GCTTCTCCCA AAGTGGCGCA GTCGGTCATA TTCAAGCCGC 201 AGCCGTCCAA ATCGCGGCGG ATTTGCGGCG CATCGACACC AATCAAGAAC 251 ACGCCTTTTG CCTTGCCTAC CAGTGCATCG CGCAGGGGCG TGAAGTCCTG 301 CCCTTTACCC ATGCCGCCCA AAATCACGAA GAGCGGATTT TGCAAACCGG 351 CAATCGCGGC GGCAGTCGCG CCGACATTCG TGCCTTTGCT GTCGTCGATA 401 AACACCACGC CGTTTTTCTC GCCGATTTTT TCCACGCGGT GCGGCAGGCC 451 TTGGAAGGTT TTGACGTGTT CGAGCAATGC TTCGCGCGAC AAACCGATGG 501 CCTCACACAA AGCCACGGCA GCCATGACGT TAGCGGCGTT GTGCAGACCT 551 TGCAACGGAA TGTCTTGCGT GACAATCAAA TCTTCATTGC CTTGTTTCAG 601 GCGGCCTGTC TCGCGTTCCA ACCAGAAATC AGCTTCGTGT TCCAACGAAA 651 ACCATTTTAC CTCGCGCCCG GCACGCTTCA TCGCGCGGCA GAACGCATCG 701 TCCGCATTCA AAACCTGCAC GCCGTCGCCA CGGAAAATCT TGGCTTTGGT 751 ATGCGCATAG TCGAGCAAGT CGTCGTAGCG GTCGAGATGG TCTTCGGAAA 801 TGTTCAGCAC CGTCGCCGCA GTCGGACGCA GGCTTTCGGT GTTTTCCAGT 851 TGGAAGCTGG AAAGCTCCAA CACCCACACG TCCGCCTTTT TGCCTTCGCG 901 CTGCCATTCC GCCTCCAAAA CCGGCGTGCC GATATTGCCC GCGATAACGG 951 TATCCAGCCC GCACTTGATA CAGAGATAGC CGACCAGGCT CGTTACCGTG 1001 GTTTTGCCGT TGCTGCCGGT AATCGCAATT ACCTTGTCGT CCCGGCGGTT 1051 CACAATGTCC GCCAGCAATT CGATGTCGCC CAACACGCGT CCGCCGTTTT 1101 GCTTGAACGC CTCAATATCC GGCTGCCGCT CGCTGATGCC GGGACTGAGA 1151 GCCAGAATAT CGAAACCGTT GTCCAGCGCA TCTTTCAGAC GGCCCGTGTA 1201 AAACACCAAC CCGTCAAACA TCTTACCGAT TTGCGACACG CGTTCCGGCT 1251 TCAGCTCCGC ATCATACGCA GCAACCTCCG CGCCGTTTTT GCGCAGGTAG 1301 GCAATCATGG AAATACCCGT ACCGCCGAGT CCGGCGACGA GGATTTTTTT 1351 GTTTTGAAAA GTCATTTTGG TTTGTCCTAA This corresponds to the amino acid sequence <SEQ ID 3; ORF 090- 1>: m090-l.pep 1 MTAFAFQTAS QSLKRFDKHF RTVRVAFEHI KARAGGAEQH NIACFGLGIC 51 RLΝGFSQSGA VGHIQAAAVQ IAADLRRIDT NQEHAFCLAY QCIAQGREVL 101 PFTHAAQΝHE ERILQTGNRG GSRADIRAFA VVDKHHAVFL ADFFHAVRQA 151 LEGFDVFEQC FARQTDGLTQ ΞHGSHDVSGV VQTLQRNVLR DNQIFIALFQ 201 AACLAFQPEI SFVFQRKPFY LAPGTLHRAA ERIVRIQNLH AVATENLGFG 251 MRIVEQVVVA VEMVFGNVQH RRRΞRTQAFG VFQLEAGKLQ HPHVRLFAFA 301 LPFRLQΝRRA DIARDNGIQP ALDTEIADQA RYRGFAVAAG NRNYLVVPAV 351 HNVRQQFDVA QHASAVLLER LNIRLPLADA GTESQNIETV VQRIFQTARV 401 KHQPVKHLTD LRHAFRLQLR II ΞNLRAVF AQVGNHGNTR TAESGDEDFF 451 VLKSHFGLS* The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 303>: g091. seq 1 ATGGAAATAC CCGTGCCGCC AAGTCCGGCG ACGAGGATTT TTTTGTTTGA 51 AAGTCATTTT GGTTTTGTCC TAAAACAAAT CATATTGGGC AGGAGACGTC 101 CGCCCTTGCC CAAGCCGCTT TCAGACGGCA TCGCGAGCCG ATTAATAACC 151 CGCCTTCAGG CGTTGGTCAT TGTCGCAGCT GTTTTGGTCT CCGTTTTGAC 201 AAGCCTTGCC AAGCCATTGT TGAGCGAGCG CAAGGTCTTG GCGCACGCCG 251 CGTCCATCGT AATACATCAA GCCCAAATTG TATTGGGCTT GGGCATCCCC 301 TTGTTCTGA This corresponds to the amino acid sequence <SEQ ID 304; ORF 091.ng>: g091.pep 1 MEIPVPPSPA TRIFLFESHF GFVLKQIILG RRRPPLPKPL SDGIASRLIT 51 RLQALVIVAA VLVSVLTSLA KPLLSERKVL AHAASIVIHQ AQIVLGLGIP 101 LF* The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 305>: m091. seq 1 ATGGAAATAC CCGTACCGCC GAGTCCGGCG ACGAGGATTT TTTTGTTTGA 51 AAAGTCATTT TGGTTTGTCC TAAAACAAAT CATATTGAGC AGGAGATGTC 101 CGCCCCTGCC CAAGCCGCTT TCAGACGGCA TCGCGAGCTG TTCAATAACC 151 CGCCTTCAGG CGTTGGTCAT TGTCGCAGCC GTCTTGGTCT CCGTTTTGAC 201 AAGCCTTGCC AAACCATTCT TGTGCAAGGG CGCGGTCTTG GCGCACGCCG 251 CGTCTTTCGG CATACATCAC GCCCAAATTG TTTTGGGCTT GGGCTACCCC 301 CTGCGC... This corresponds to the amino acid sequence <SEQ ID 306; ORF 091>: m091.pep 1 MEIPVPPSPA TRIFLFEKSF WFVLKQIILS RRCPPLPKPL SDGIASCSIT 51 RLQALVIVAA VLVSVLTSLA KPFLCKGAVL AHAASFGIHH AQIVLGLGYP 101 LR. Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 091 shows 84.2% identity over a 101 aa overlap with a predicted ORF (ORF 091.ng) from N gonorrhoeae: m091/g091 10 20 30 40 50 60 m091.pep MEIPVPPSPATRIFLFEKSFWFVLKQIILSRRCPPLPKPLSDGIASCSITRLQALVIVAA I I I M M M M I I M M I M g091 MEIPVPPSPATRIFLFESHFGFVLKQI ILGRRRPPLPKPLSDGIASRLITRLQALVIVAA 10 20 30 40 50 60 70 80 90 100 mO 91 . ep VLVSVLTSLAKPFLCKGAVLAHAASFGIHHAQIVLGLGYPLR M M M M M M M = M M I I M I M I I g091 VLVSVLTSLAKPLLSERKVLAHAASIVIHQAQIVLGLGIPLFX 70 80 90 100 The following partial DΝA sequence was identified in N meningiditis <SEQ ID 307>: a091.seq 1 ATGGAAATAC CCGTGCCGCC AAGTCCGGCG ACGAGGATTT TTTTGTTTTG 51 GAAATCATTT TGGTTTGTCC TAAAACAAAT CATATTGAGC AGGGGATGTC 101 TGATCCTGCT CAAGCCGCTT TCAGACGGCA TCGCGAGCTG TTCAATAACC 151 CGCTTTCAGG CGTTGGTCAT TGTCGCAGCT GTCTTGGTAT CCGTTTTGAC 201 AAGCCTTGCC AAGCCATTCT TGTGCAAGGG CGCGGTCTTG GCGCACGCCG 251 CGTCTTTCGG CATACATCAC GCCCAAATTG TTTTGGGC This corresponds to the amino acid sequence <SEQ ID 308; ORF 091. a>: a091.pep 1 MEIPVPPSPA TRIFLFWKSF WFVLKQIILS RGCLILLKPL SDGIASCSIT 51 RFQALVIVAA VLVSVLTSLA KPFLCKGAVL AHAASFGIHH AQIVLG m09i/a09i 93.8%) identity over a 96 aa overlap 10 20 30 40 50 60 m091.pep MEIPVPPSPATRIFLFEKSFWFVLKQIILSRRCPPLPKPLSDGIASCSITRLQALVIVAA I I I I I II I II II II II II I II I II II II II I I II I II I II I II II I : II I I II I I a091 MEIPVPPSPATRIFLFWKSFWFVLKQIILSRGCLILLKPLSDGIASCSITRFQALVIVAA 10 20 30 40 50 60 70 80 90 100 m091.pep VLVSVLTSLAKPFLCKGAVLAHAASFGIHHAQIVLGLGYPLR I I I I II II II II II II I I I I II I I I II II II II I II a091 VLVSVLTSLAKPFLCKGAVLAHAASFGIHHAQIVLG 70 80 90 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 309>: g092.seq 1 ATGTTTTTTA TTTCAATCCG CTATATATTT GTCAGAAAAC TATGGTGCGC 51 AAACGGTCAG ACCTTTAAAA TAACGCCTTT ACGCACTAAA AACCAACCGG 101 AACGCAACAT TATGATGAAA AATCGAGTAA GCAACATCCA TTTTGTCGGT 151 ATCGGCGGCG TCGGCATGAG CGGTATCGCC GAAGTCTTGC ACAATTTGGG 201 CTTTAAAGTT TCCGGTTCGG ATCAGGCGCG AAATGCCGCT ACCGAGCATT 251 TGAGCAGCCT GGGCATTCAA GTTTATCCCG GCCATACCGC AGAACACGTT 301 AACGGTgcgg ATGTCGTCGT TGCCTCTACC GCCGTCAAGA AAGAAaatcC 351 CGAAGTtgtc gcTGCGTTGG AGCGGCAAAT TCCCGTTATT CCGCGCGCCT 401 TGATGCTGGC AGAGCTGATG CGCTTCCGTG ACGgcatcgc cattgccggT 451 ACGCACGGCA AAACCACGAC CACCAGCCTG ACCGCCTCCA TCCTCGGCGC 501 GGCAGGACTC GACCCCACTT TCGTTATCGG CGGCAAACTC AACGCCGCAG 551 GCACCAACGC CCGCTTGGGC AAAGGCGAAT ACATCGTTGC CGAAGCCGAC 601 GAATCCGATG CCTCTTTCCT ACATCTGACC CCGATTATGT CCGTCGTTAC 651 CAATATCGAC GAAGACCATA TGGATACCTA CGGGCACAGC GTCGAAAAAC 701 TGCATCAGGC GTTTATCGAT TTCATCCACC GTATGCCCTT CTACGGCAAA 751 GCCTTTTTGT GTGTTGACAG CGAACACGTC CGCGCGATTT TGCCCAAAGT 801 GAGCAAACCT TATGCTACTT ACGGTTTGGA CGATACCGCC GACATCTACG 851 CCACCGACAT CGAAAACGTC GGCGCGCAAA TGAAATTCAC CGTCCATGTT 901 CAAATGAAAG GACATGAGCA GGGGTCGTTT GAAGTCGTGC TGAATATGCC 951 CGGCAGACAC AACGTGCTGA ACGCATTGGC AGCCATCGGc gtggcGCTgg 1001 aagtcGgCGC ATcggttgAA GCGAtcCAAA AaggCTTGCT CGGCTTTGAA 1051 GGCGTCGGCC GCCGCTTCCA AAAATAcggc gacatCAagt tgccaaacgg 1101 cggGaccgCT TTgctGGTGG ACGATTAcgg ACACCACCCC GTCGAAATGG 1151 CGGcaaccct tgccgcTGCA CGCGGCGCGT ATCCGGAAAA acgtTTGGTG 1201 CtcgCCTTCC AGCCGCACCG CTATACCCGC ACGCGCGATT TGTTTGAAGA 1251 CTTTACCAAA GTACTCAATA CCGTTGatgC GCTGGTACTG ACCGAAGTTT 1301 AtgccgccgG CGAAGAGCCG GTTGCCGCCG CCGactcCCG CGCCTTGGCG 1351 CGTGCTATCC GCGTATTGGG CAAACTTGAG CCGATTTACT GCGAAAatgt 1401 cgccgACCTG CCGCAAATGC TGATGAATGT TTTACAGGAT Ggcgatgttg 1451 tgttgAATAT GggTgcggga agcatcaacc gcgttccttc cgcgctgttg 1501 gaattgtcga AACAGAtttg A This corresponds to the amino acid sequence <SEQ ID 310; ORF 092.ng>: g092.pep 1 MFFISIRYIF VRKLWCAΝGQ TFKITPLRTK ΝQPERΝIMMK ΝRVSΝIHFVG 51 IGGVGMSGIA EVLHΝLGFKV ΞGSDQARΝAA TEHLΞSLGIQ VYPGHTAEHV 101 ΝGADVWAST AVKKEΝPEW AALERQIPVI PRALMLAELM RFRDGIAIAG 151 THGKTTTTSL TASILGAAGL DPTFVIGGKL ΝAAGTΝARLG KGEYIVAEAD 201 ESDASFLHLT PIMSWTΝID EDHMDTYGHS VEKLHQAFID FIHRMPFYGK 251 AFLCVDSEHV RAILPKVSKP YATYGLDDTA DIYATDIENV GAQMKFTVHV 301 QMKGHEQGSF EWLNMPGRH NVLNALAAIG VALEVGASVE AIQKGLLGFE 351 GVGRRFQKYG DIKLPNGGTA LLVDDYGHHP VEMAATLAAA RGAYPEKRLV 401 LAFQPHRYTR TRDLFEDFTK VLNTVDALVL TEVYAAGEEP VAAADSRALA 451 RAIRVLGKLE PIYCENVADL PQMLMNVLQD GDWLNMGAG SINRVPSALL 501 ELSKQI* The following partial DNA sequence was identified in N meningitidis <SEQ ID 311>: m092.seq 1 ATGTTTTTTA TTTCAATCCG CTATATATTT GTCAGAAAAC TATGGCGCGC 51 AAACGGTCAG CCCTTTAAAA TAACGCCTTT ACGCATCGAA AATCCACCGG 101 AACGCAACAT TATGATGAAA AATCGAGTTA CCAACATCCA TTTTGTCGGT 151 ATCGGCGGCG TCGGCATGAG CGGCATCGCC GAAGTCTTGC ACAATTTGGG 201 CTTTAAAGTT TCCGGTTCGG ATCAgGCGCG AAATGCCGCT ACCGAGCATT 251 TGGGCAGCCT GGGCATTCAA GTTTATCCCG GCCATACCGC CGAACACGTT 301 AACGGTGCGG ATGTCGTCGT TACCTCTACC GCCGTCAAAA AAGAAAATCC 351 CGAAGTTGTC GCTGCGTTGG AGCAGCAAAT TCCCGTTATT CCGCGCGCCC 401 TGATGTTGGC GGAGTTGATG CGCTTCCGTG ACGGCATCGC CATTGCCGGC 451 ACGCACGGCA AAACCACGAC CACCAGCCTG ACCGCCTCCA TCCTCGGCGC 501 GGCAGGACTT GACCCGACTT TCGTTATCGG CGGCAAACTC AACGCCGCAG 551 GCACTAACGC CCGCTTGGGC AAAGGCGAAT ACATCGTTGC CGAAGCCGAC 601 GAGTCGGATG CATCCTTTCT GCACCTGACA CCGATTATGT CCGTCGTTAC 651 CAATATCGAC GAAGACCATA TGGATACCTA CGGGCACAGC GTCGAAAAAC 701 TGCATCAGGC GTTTATCGAT TTCATCCACC GTATGCCCTT CTACGGCAAA 751 GCCTTTTTGT GTATTGACAG CGAACACGTC CGCGCGATTT TGCCCAAAGT 801 GAGCAAACCT TATGCTACTT ACGGTTTGGA CGATACCGCC GACATCTACG 851 CCACCGACAT CGAAAACGTC GGCGCGCAAA TGAAATTCAC CGTCCATGTT 901 CAAATGAAAG GACATGAGCA GGGGTCGTTT GAAGTCGTGC TGAATATGCC 951 CGGCAGACAC AACGTGCTGA ACGCATTGGC AGCCATCGGC GTGGCGCTGG 1001 AAGTCGGCGC ATCGGTTGAA GCGATCCAAA AAGGCTTGCT CGGCTTTGAA 1051 GGCGTCGGCC GCCGCTTCCA AAAATACGGC GACATCAAGT TGCCAAACGG 1101 CGGGACCGCG CTCTTGGTGG ACGACTACGG ACACCACCCC GTCGAAATGG 1151 CGGCGACCCT TGCCGCCGCA CGCGGCGCGT ATCTGGAAAA ACGTTTGGTA 1201 CTCGCCTTCC AGCCGCACCG CTATACCCGC ACGCGCGATT TGTTTGAAGA 1251 CTTTACCAAA GTCCTCAATA CCGTTGACGC GCTGGTGCTG ACCGAAGTTT 1301 ATGCCGCCGG TGAAGAGCCG ATTGCCGCCG CCGATTCCCG CGCTCTTGCC 1351 CGCGCCATCC GCGTGTTGGG CAAACTCGAG CCGATTTACT GCGAAAACGT 1401 TGCCGATCTG CCCGAAATGC TGTTGAACGT TTTGCAGGAC GGCGACATCG 1451 TGTTGAATAT GGGCGCGGGA AGCATCAACC GCGTCCCCGC CGCGCTGCTG 1501 GCATTGTCGA AACAGATTTG A This corresponds to the amino acid sequence <SEQ ID 312; ORF 092>: m092.pep 1 MFFISIRYIF VRKLWRAΝGQ PFKITPLRIE ΝPPERΝIMMK ΝRVTΝIHFVG 51 IGGVGMSGIA EVLHΝLGFKV SGSDQARΝAA TEHLGSLGIQ VYPGHTAEHV 101 ΝGADVWTST AVKKEΝPEW AALEQQIPVI PRALMLAELM RFRDGIAIAG 151 THGKTTTTSL TASILGAAGL DPTFVIGGKL ΝAAGTΝARLG KGEYIVAEAD 201 ESDASFLHLT PIMSWTΝID EDHMDTYGHS VEKLHQAFID FIHRMPFYGK 251 AFLCIDSEHV RAILPKVSKP YATYGLDDTA DIYATDIENV GAQMKFTVHV 301 QMKGHEQGSF EWLNMPGRH NVLNALAAIG VALEVGASVE AIQKGLLGFE 351 GVGRRFQKYG DIKLPNGGTA LLVDDYGHHP VEMAATLAAA RGAYLEKRLV 401 LAFQPHRYTR TRDLFEDFTK VLNTVDALVL TEVYAAGEEP IAAADSRALA 451 RAIRVLGKLE PIYCENVADL PEMLLNVLQD GDIVLNMGAG SINRVPAALL 501 ALSKQI* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N. gonorrhoeae ORF 092 shows 96.6% identity over a 506 aa overlap with a predicted ORF (ORF 092.ng) from N gonorrhoeae: m092/g092 10 20 30 40 50 60 m092.pep MFFISIRYIFVRKLWRAΝGQPFKITPLRIEΝPPERΝIMMKΝRVTΝIHFVGIGGVGMSGIA g092 MFFISIRYIFVRKLWCANGQTFKITPLRTKNQPERNIMMKNRVSNIHFVGIGGVGMSGIA 10 20 30 40 50 60 70 80 90 100 110 120 m092.pep EVLHNLGFKVSGSDQARNAATEHLGSLGIQVYPGHTAEHVNGADVWTSTAVKKENPEW MMMMMMMMMMMMMMMMMMMMMMMIMMMIMMM g092 EVLHNLGFKVSGSDQARNAATEHLSSLGIQVYPGHTAEHVNGADVWASTAVKKENPEW 70 80 90 100 110 120 130 140 150 160 170 180 m092.pep AALEQQIPVIPRALMLAELMRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKL MMMMMMMMMMMIMMMMMMMMMMMMMMMIMMM g092 AALERQIPVIPRALMLAELMRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKL 130 140 150 160 170 180 190 200 210 220 230 240 m092. pep NAAGTNARLGKGEYIVAEADESDASFLHLTPIMSWTNIDEDHMDTYGHSVEKLHQAFID g092 NAAGTNARLGKGEYIVAEADESDASFLHLTPIMSWTNIDEDHMDTYGHSVEKLHQAFID 190 200 210 220 230 240 250 260 270 280 290 300 mO92. pep FIHRMPFYGKAFLCIDSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHV MMMMMMMMMMMMIMMMMMMMMMMMMMMIMMM g092 FIHRMPFYGKAFLCVDSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHV 250 260 270 280 290 300 310 320 330 340 350 360 m092 . pep QMKGHEQGSFEWLNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYG M M M M M M M I M M M I I M M M M M M M M M M M M M M M I M M M g 092 QMKGHEQGS FEWLNMPGRHNVLNALAAI GVALEVGASVEAI QKGLLGFEGVGRRFQKYG 310 320 330 340 350 360 370 380 390 400 410 420 mO 92 . pep DIKLPNGGTALLVDDYGHHPVEMAATLAAARGAYLEKRLVLAFQPHRYTRTRDLFEDFTK g092 DIKLPNGGTALLVDDYGHHPVEMAATLAAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTK 370 380 390 400 410 420 430 440 450 460 470 480 m092.pep VLNTVDALVLTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQD MMMMMMMMMMMIMMIMMIMMMMMMMMMMIMMM g092 VLNTVDALVLTEVYAAGEEPVAAADSRALARAIRVLGKLEPIYCENVADLPQMLMNVLQD 430 440 450 460 470 480 490 500 m092.pep GDIVLNMGAGSINRVPAALLALSKQIX M M M M M M M M M M l l l l l l g092 GDWLNMGAGSINRVPSALLELSKQIX 490 500 The following partial DNA sequence was identified in N meningitidis <SEQ ID 313>: a092.seq 1 ATGTTTTTTA TTTCAATCCG CTATATATTT GTCAGAAAAC TATGGCGCGC 51 AAACGGTCAG CCCTTTAAAA TAACGCCTTT ACGCATCGAA AATCCACCGG 101 AACGCAACAT TATGATGAAA AATCGAGTGA CCAACATCCA TTTTGTCGGT 151 ATCGGCGGCG TCGGCATGAG CGGTATCGCC GAAGTCTTGC ACAATTTGGG 201 TTTTAAAGTT TCCGGTTCGG ATCAGGCGCG AAATGCCGCT ACCGAGCATT 251 TGGGCAGCCT GGGCATTCAA GTTTATCCCG GCCATACCGC AGAACACGTT 301 AACGGTGCGG ATGTCGTCGT TACCTCTACC GCCGTCAAAA AAGAAAATCC 351 CGAAGTTGTC GCTGCGTTGG AGCAGCAAAT TCCCGTTATT CCGCGCGCCC 401 TGATGTTGGC GGAGTTGATG CGCTTCCGTG ACGGCATCGC CATTGCCGGC 451 ACGCACGGCA AAACCACGAC CACCAGCCTG ACCGCCTCCA TCCTCGGCGC 501 GGCAGGACTT GACCCGACTT TCGTTATCGG CGGCAAACTC AACGCCGCAG 551 GCACCAACGC CCGCTTGGGC AAAGGCGAAT ACATCGTTGC CGAAGCCGAC 601 GAGTCGGATG CATCCTTTCT GCACCTGACA CCGATTATGT CCGTCGTTAC 651 CAATATCGAC GAAGACCATA TGGATACCTA CGGGCACAGT GTTGAGAAGC 701 TGCATCAGGC GTTTATCGAT TTCATCCACC GTATGCCCTT CTACGGCAAA 751 GCCTTTTTGT GTATTGACAG CGAACACGTC CGCGCGATTT TGCCCAAAGT 801 GAGCAAACCT TATGCTACTT ACGGTTTGGA CGATACCGCC GACATCTACG 851 CCACCGACAT CGAAAACGTC GGCGCGCAAA TGAAATTCAC CGTCCATGTT 901 CAAATGAAAG GACATGAGCA GGGGTCGTTT GAAGTCGTGC TGAATATGCC 951 CGGCAGACAC AACGTGCTGA ACGCATTGGC AGCCATCGGC GTGGCGCTGG 1001 AAGTCGGCGC ATCGGTTGAA GCGATCCAAA AAGGCTTGCT CGGCTTTGAA 1051 GGTGTCGGCC GCCGCTTCCA AAAATACGGC GACATCAAGT TGCCAAACGG 1101 TGGAACCGCG CTCTTGGTGG ACGACTACGG ACACCACCCC GTCGAAATGG 1151 CGGCGACCCT TTCCGCCGCA CGCGGCGCGT ATCCGGAAAA ACGTTTGGTA 1201 CTCGCCTTCC AGCCGCACCG CTATACCCGC ACGCGCGATT TGTTTGAAGA 1251 CTTTACCAAA GTCCTCAATA CCGTTGACGC GCTGGTGCTG ACCGAAGTTT 1301 ATGCCGCCGG TGAAGAGCCG ATTGCCGCCG CTGATTCCCG CGCTCTTGCC 1351 CGCGCCATCC GCGTGTTGGG CAAACTCGAG CCGATTTACT GCGAAAACGT 1401 TGCCGATCTG CCCGAAATGC TGTTGAACGT TTTGCAGGAC GGCGACATCG 1451 TGTTGAATAT GGGTGCGGGA AGCATCAACC GCGTCCCCGC CGCGCTGCTG 1501 GAATTGTCGA AACAGATTTG A This corresponds to the amino acid sequence <SEQ ID 314; ORF 092. a>: a092.pep 1 MFFISIRYIF VRKLWRANGQ PFKITPLRIE NPPERNIMMK NRVTNIHFVG 51 IGGVGMSGIA EVLHNLGFKV SGSDQARNAA TEHLGSLGIQ VYPGHTAEHV 101 NGADVWTST AVKKENPEW AALEQQIPVI PRALMLAELM RFRDGIAIAG 151 THGKTTTTSL TASILGAAGL DPTFVIGGKL NAAGTNARLG KGEYIVAEAD 201 ESDASFLHLT PIMSVVTNID EDHMDTYGHS VEKLHQAFID FIHRMPFYGK 251 AFLCIDSEHV RAILPKVSKP YATYGLDDTA DIYATDIENV GAQMKFTVHV 301 QMKGHEQGSF EWLNMPGRH NVLNALAAIG VALEVGASVE AIQKGLLGFE 351 GVGRRFQKYG DIKLPNGGTA LLVDDYGHHP VEMAATLSAA RGAYPEKRLV 401 LAFQPHRYTR TRDLFEDFTK VLNTVDALVL TEVYAAGEEP IAAADSRALA 451 RAIRVLGKLE PIYCENVADL PEMLLNVLQD GDIVLNMGAG SINRVPAALL 501 ELSKQI* m092/a092 99.4%o identity over a 506 aa overlap 10 20 30 40 50 60 m092 •pep MFFISIRYIFVRKLWRANGQPFKITPLRIENPPERNIMMKNRVTNIHFVGIGGVGMSGIA I II II I I I II I II II I II II II II I II I II I II II I II I II I II II I II I I I I II I II I I a092 MFFISIRYIFVRKLWRANGQPFKITPLRIENPPERNIMMKNRVTNIHFVGIGGVGMSGIA 10 20 30 40 50 60 70 80 90 100 110 120 mO 92. pep EVLHNLGFKVSGSDQARNAATEHLGSLGIQVYPGHTAEHVNGADVWTSTAVKKENPEW I II I II II II I II II I II II II II I II I II I II II I I II I II I II I II I II II II II I II a092 EVLHNLGFKVSGSDQARNAATEHLGSLGIQVYPGHTAEHVNGADWVTSTAVKKENPEW 70 80 90 100 110 120 130 140 150 160 170 180 m092.pep AALEQQIPVIPRALMLAELMRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKL II II I II II M I I II II I II I I II II II I I II II I II I I II II II I II II II II I II I II a092 AALEQQIPVIPRALMLAELMRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKL 130 140 150 160 170 180 190 200 210 220 230 240 m092.pep NAAGTNARLGKGEYIVAEADESDASFLHLTPIMSWTNIDEDHMDTYGHSVEKLHQAFID I II II I I I I M M I I I I II II I II II II I I II I I I II I I I II II II I I M II II II II I I a092 NAAGTNARLGKGEYIVAEADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFID 190 200 210 220 230 240 250 260 270 280 290 300 m092.pep FIHRMPFYGKAFLCIDSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHV I I I I II I II I I II II I II I II II I II II II II I II II II II II II II I II II I I II I II I a092 FIHRMPFYGKAFLCIDSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHV 250 260 270 280 290 300 310 320 330 340 350 360 mO92. pep QMKGHEQGSFEWLNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYG II I I II I II I I II II I II I II II I II II II II I II II II II II II II II I II II II II II a092 QMKGHEQGSFEWLNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYG 310 320 330 340 350 360 370 380 390 400 410 420 m092.pep DIKLPNGGTALLVDDYGHHPVEMAATLAAARGAYLEKRLVLAFQPHRYTRTRDLFEDFTK I II I II II I I II II I II II I II II II I: II II I I II II I II I II II II I II II I I II I I a092 DIKLPNGGTALLVDDYGHHPVEMAATLSAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTK 370 380 390 400 410 420 430 440 450 460 470 480 m092.pep VLNTVDALVLTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQD II II I I II I II I II I II II I II II I I II I II II II II I I I II II II II II I I I I I II II I a092 VLNTVDALVLTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQD 430 440 450 460 470 480 490 500 m092.pep GDIVLNMGAGSINRVPAALLALSKQIX I I I I I II II I I II I II II II llllll a092 GDIVLNMGAGSINRVPAALLELSKQIX 490 500 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 315>: g093.seq 1 aTGCAGAATt ttgGCAAAGT ggccgtATTG ATGGGtggtT TTTCCAGCGA 51 ACGAGAaatC tcgcTGGACA GCgGTACCGC CATTTTGAAC GCCTTAAAAA 101 GCAAAGGCAT AGACGCATAC GCCTTCGACC CTAAGGAAAC GCCGTTATCC 151 GAACTGAAGG AGCGGGGCTT TCAGACGGCA TTCAACATCC TTCACGGTAC 201 TTACGGCGAA GACGGGGCTG TTCAGGGTGC ATTGGAACTG TTGGGCATTC 251 CCTATACCGG CAGCGGTGTC GCCGCCTCCG CCATCGGCAT GGACAAATAC 301 CGCTGCAAAC TGATTTGGCA GGCATTGGGA TTACCCGTTC CCGAGTTCGC 351 CGTACTGTAC GATGATACCG ATTTCGATGC CGTCGAAGAA AAATTGGGTC 401 TGCCGATGTT TGTGAAGCCG GCGGCCGAAG GCAGCAGCgt cggcgtggta 451 aAAGTCAAAG AAAaaggccg TCTGAAAAGC GTTtacgaag aatTGAaaCA 501 CCTTcagggg cgaAAtcatt gccgAacgTT TTATCGGCGG CGGCGAATAT 551 TCCTGCCCCG TCCTGAACGG CAAAGGGCTG CCCGGCATAC ACATCATCCC 601 CGCAACCGAG TTTTACGAct acgaagccaa GtacaaCCGA GACGAcacca 651 tttaTCAATG TCCTTCGGAA GATTTGACCG AAGCCGAAGA AAGCCTGATG 701 CGCGAACTGG CGGTTCGCGG CGCACAGGCA ATCGGTGCGG AAGGCTGCGT 751 GCGCGTCGAT TTCCTCAAAG ATACCGACGG CAAACTCTAT CTGTTGGAAA 801 TCAACACCCT GCCCGGTATG ACCGGCCATA G This corresponds to the amino acid sequence <SEQ ID 316; ORF 093.ng>: g093.pep 1 MQΝFGKVAVL MGGFSSEREI SLDSGTAILΝ ALKSKGIDAY AFDPKETPLS 51 ELKERGFQTA FΝILHGTYGE DGAVQGALEL LGIPYTGSGV AASAIGMDKY 101 RCKLIWQALG LPVPEFAVLY DDTDFDAVEE KLGLPMFVKP AAEGSSVGW 151 KVKEKGRLKS VYEELKHLQG RΝHCRTFYRR RRIFLPRPER QRAARHTHHP 201 RΝRVLRLRSQ VQPRRHHLSM SFGRFDRSRR KPDARTGGSR RTGΝRCGRLR 251 ARRFPQRYRR QTLSVGΝQHP ARYDRP* The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 317>: m093 . seq 1 ATGCAGAATT TTGGCAAAGT GGCCGTATTG ATGGGCGGTT TTTCCAGCGA 51 ACGAGAAATC TCGCTGGACA GCGGCACCGC CATTTTGAAT GCTTTAAAAA 101 GCAAAGGCAT AGACGCATAC GCCTTCGATC CTAAAGAAAC CCCATTGTCT 151 GAATTGAAGG CACAAGGTTT TCAGACGGCA TTCAACATCC TTCACGGTAC 201 TTACGGCrAA GACGGGGCGG TTCAGGGTGC ATTGGAACTG TTGGGCATTC 251 CCTATACCGG CAGCGGTGTC GCCGCATCCG CCATCGGCAT GGACAAATAC 301 CGCTGCAAAC TGATTTGGCA GGCATTGGGA TTGCCCGTTC CCGAGTTCGC 351 CGTCCTGCAC GACGACACTG ATTTCGATGC CGTCGAAGAA AAATTGGGCC 401 TGCCGATGTT TGTGAAACCG GCGGCCGAAG GCAGCAGCGT AGGCGTGGTA 451 AAAGTCAAAG GAAAAGGCCG TCTGAAAAGC GTTTACGAAG AATTGAAACA 501 CCTTCAGGG. CGAAATCATT GCCGAACGTT TTATCGGCGG CGGCGAATAT 551 TCCTGCCCCG TCCTGAACGG CAAAGGGCTG CCCGGCATAC ACATCATTCC 601 CGCAACCGAG TTTTACGACT ACGAAGCCAA GTACAACCGC GACGACACCA 651 TTTATCAATG TCCTTCGGAA GATTTGACCG AAGCCGAAGA AAGCCTGATG 701 CGCGAACTGG CGGTTCGCGG CGCGCAGGCA ATCGGTGCGG AAGGCTGCGT 751 GCGCGTCGAT TTCCTCAAAG ATACCGACGG CAAACTCTAT CTGTTGGAAA 801 TCAACACCCT GCCCGGTATG ACGAGCCATA G This corresponds to the amino acid sequence <SEQ ID 318; ORF 093>: m093.pep 1 MQNFGKVAVL MGGFSSEREI SLDSGTAILN ALKSKGIDAY AFDPKETPLS 51 ELKAQGFQTA FNILHGTYGX DGAVQGALEL LGIPYTGSGV AASAIGMDKY 101 RCKLIWQALG LPVPEFAVLH DDTDFDAVEE KLGLPMFVKP AAEGSSVGW 151 KVKGKGRLKS VYEELKHLQX RNHCRTFYRR RRIFLPRPER QRAARHTHHS 201 RNRVLRLRSQ VQPRRHHLSM SFGRFDRSRR KPDARTGGSR RAGNRCGRLR 251 ARRFPQRYRR QTLSVGNQHP ARYDEP* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 093 shows 96.7% identity over a 276 aa overlap with a predicted ORF (ORF 093. ng) from N gonorrhoeae: m093/g093 10 20 30 40 50 60 m093. pep MQΝFGKVAVLMGGFSSEREISLDSGTAILΝALKSKGIDAYAFDPKETPLSELKAQGFQTA MUM g093 MQΝFGKVAVLMGGFSSEREISLDSGTAILΝALKSKGIDAYAFDPKETPLSELKERGFQTA 10 20 30 40 50 60 70 80 90 100 110 120 m093. pep FΝILHGTYGXDGAVQGALELLGIPYTGSGVAASAIGMDKYRCKLIWQALGLPVPEFAVLH g093 FΝILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRCKLIWQALGLPVPEFAVLY 70 80 90 100 110 120 130 140 150 160 170 180 m093 . pep DDTDFDAVEEKLGLPMFVKPAAEGSSVGWKVKGKGRLKSVYEELKHLQXRΝHCRTFYRR g093 DDTDFDAVEEKLGLPMFVKPAAEGSSVGWKVKEKGRLKSVYEELKHLQGRΝHCRTFYRR 130 140 150 160 170 180 190 200 210 220 230 240 m093 . pep RRIFLPRPERQRAARHTHHSRΝRVLRLRSQVQPRRHHLSMSFGRFDRSRRKPDARTGGSR g093 RRIFLPRPER Q RAARHTHHPRΝRVLRLRSQVQPRRHHLSMSFGRFDRSRRKPDARTGGSR 190 200 210 220 230 240 250 260 270 mO93. pep RAGΝRCGRLRARRFPQRYRRQTLSVGΝQHPARYDEPX IMMMMMMMMMMMMMMIMMMI g093 RTGΝRCGRLRARRFPQRYRRQTLSVGΝQHPARYDRPX 250 260 270 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 319>: a093.seq 1 ATGCAGAATT TTGGCAAAGT GGCCGTATTG ATGGGCGGTT TTTCCAGCGA 51 ACGAGAAATC TCGCTGGACA GCGGCACCGC CATTTTGAAT GCTTTAAAAA 101 GCAAAGGCAT AGACGCATAC GCCTTCGATC CCAAGGAAAC CCCATTGTCT 151 GAATTGAAGG CACAAGGTTT TCAGACGGCA TTCAACATCC TTCACGGTAC 201 TTACGGCGAA GACGGGGCTG TTCAGGGTGC ATTGGAACTG TTGGGCATTC 251 CCTATACCGG CAGCGGTGTC GCCGCATCCG CCATCGGCAT GGACAAATAC 301 CGCTGCAAAC TGATTTGGCA GGCATTGGGA TTGCCCGTTC CCGAGTTCGC 351 CGTCCTGCAC GACGACACTG ATTTCGATGC CGTCGAAGAA AAATTGGGCC 401 TGCCGATGTT TGTGAAACCG GCGGCCGAAG GCAGCAGCGT AGGCGTGGTA 451 AAAGTCAAAG GAAAAGGCCG TCTGAAAAGC GTTTACGAAG AATTGAAACA 501 CTTTCAGGG. CGAAATCATT GCCGAACGGT TTATCGGCGG CGGCGAATAT 551 TCCTGCCCTG TGTTGAACGG CAAAGGCCTG CCCGGCATAC ACATCATCCC 601 CGCGACCGAG TTTTATGACT ACGAAGCCAA GTACAACCGC AACGACACCA 651 TTTATCAATG TCCTTCGGAA GATCTGACCG AAGCCGAAGA AAGCCTGATG 701 CGCGAACTGG CGGTTCGCGG CGCGCAGGCA ATCGGTGCGG AAGGCTGCGT 751 GCGCGTCGAT TTCCTCAAAG ATACCGACGG CAAACTCTAT CTGTTGGAAA 801 TCAACACCCT GCCCGGTATG ACCGGCCATA G This corresponds to the amino acid sequence <SEQ ID 320; ORF 093. a>: a093.pep MQNFGKVAVL MGGFSSEREI SLDSGTAILN ALKSKGIDAY AFDPKETPLS 51 ELKAQGFQTA FNILHGTYGE DGAVQGALEL LGIPYTGSGV AASAIGMDKY 101 RCKLIWQALG LPVPEFAVLH DDTDFDAVEE KLGLPMFVKP AAEGSSVGW 151 KVKGKGRLKS VYEELKHFQX RNHCRTVYRR RRIFLPCVER QRPARHTHHP 201 RDRVL*LRSQ VQPQRHHLSM SFGRSDRSRR KPDARTGGSR RAGNRCGRLR 251 ARRFPQRYRR QTLSVGNQHP ARYDRP* m093/a093 95.7%o identity over a 276 aa overlap 10 20 30 40 50 60 m093 •pep MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDPKETPLSELKAQGFQTA I II II II I II II II II II I II I II I II I II I II I I I I II II II II II I I I II I I I II I II a093 MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDPKETPLSELKAQGFQTA 10 20 30 40 50 60 70 80 90 100 110 120 mO 93. pep FNILHGTYGXDGAVQGALELLGIPYTGSGVAASAIGMDKYRCKLIWQALGLPVPEFAVLH lllllllll II II II II I II I II I II I I II II I II I I I II II II I II I I II I I I M I II a093 FNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRCKLIWQALGLPVPEFAVLH 70 80 90 100 110 120 130 140 150 160 170 180 mO 93. pep DDTDFDAVEEKLGLPMFVKPAAEGSSVGWKVKGKGRLKSVYEELKHLQXRNHCRTFYRR II II I II II I I II I II II II II I II II I II I I II II I II II II II II : I I II I I I I III a093 DDTDFDAVEEKLGLPMFVKPAAEGSSVGWKVKGKGRLKΞVYEELKHFQXRNHCRTVYRR 130 140 150 160 170 180 190 200 210 220 230 240 m093 . pep RRIFLPRPERQRAARHTHHSRNRVLRLRSQVQPRRHHLSMSFGRFDRSRRKPDARTGGSR llllll II II llllll I M II I II II M I II I II I II lllllllllllllll a093 RRIFLPCVERQRPARHTHHPRDRVLXLRSQVQPQRHHLSMSFGRSDRSRRKPDARTGGSR 190 200 210 220 230 240 250 260 270 m093. pep RAGNRCGRLRARRFPQRYRRQTLSVGNQHPARYDEPX I I I I I I II II II II II II I II I II I II II II II I : I I aO 93 RAGNRCGRLRARRFPQRYRRQTLSVGNQHPARYDRPX 250 260 270 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 321>: g094. seq 1 ATGTATTCGC CTTTGCCCAA GCGGGCGTTG GTGCCTGCGG CGTTGAGTTT 51 GCCGCCGATA ACGAAAGTGG GGTCGAGTCC TGCCGCGCCG AGGATGGAGG 101 CGGTCAGGCT GGTGGTCGTG GTTTTGCCGT GCGTAccggc aatggcgatg 151 cCGTCACGGA AGCGCATCAG CTCTGCCAGC ATCAAGGCGC GCGGAATAAC 201 GGGAATTTGC CGCTCCAACG CAgcgacaAC TTCGGgattT TCTTTCTTGA 251 CGGCGGTAGA GGCAACGACG ACATccgcAC CGTTAACGTG TTCTGCGGTA 301 TGGCCGGGAT AA This corresponds to the amino acid sequence <SEQ ID 322; ORF 094.ng>: g094 -pep 1 MYSPLPKRAL VPAALSLPPI TKVGSSPAAP RMEAVRLVW VLPCVPAMAM 51 PSRKRISSAS IKARGITGIC RSΝAATTSGF SFLTAVEATT TSAPLTCSAV 101 WPG* The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 323>: m094. seq 1 ATGTATTCGC CTTTGCCCAA GCGGGCGTTA GTGCCTGCGG CGTTGAGTTT 51 GCCGCCGATA ACGAAAGTCG GGTCAAGTCC TGCCGCGCCG AGGATGGAGG 101 CGGTCAGGCT GGTGGTCGTG GTTTTGCCGT GCGTGCCGGC AATGGCGATG 151 CCGTCACGGA AGCGCATCAA CTCCGCCAAC ATCAGGGCGC GCGGAATAAC 201 GGGAATTTGC TGCTCCAACG CAGCGACAAC TTCGGGATTT TCTTTTTTGA 251 CGGCGGTAGA GGTAACGACG ACATCCGCAC CGTTAACGTG TTCGGCGGTA 301 TGGCCGGGAT AA This corresponds to the amino acid sequence <SEQ ID 324; ORF 094>: m094.pep 1 MYSPLPKRAL VPAALSLPPI TKVGSSPAAP RMEAVRLVW VLPCVPAMAM 51 PSRKRIΝSAΝ IRARGITGIC CSΝAATTSGF SFLTAVEVTT TSAPLTCSAV 101 WPG* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 094 shows 95.1% identity over a 103 aa overlap with a predicted ORF (ORF 094.ng) from N gonorrhoeae: m094/g094 10 20 30 40 50 60 m094.pep MYSPLPKRALVPAALSLPPITKVGSSPAAPRMEAVRLWWLPCVPAMAMPSRKRIΝSAΝ M M M M M M M M M M I M M M M M M M M M M M M M M M I M M M h g094 MYSPLPKRALVPAALSLPPITKVGSSPAAPRMEAVRLWWLPCVPAMAMPSRKRISSAS 10 20 30 40 50 60 70 80 90 100 mO 94 . pep IRARGITGICCSΝAATTSGFSFLTAVEVTTTSAPLTCSAVWPGX I M I I M I M M M M M M M M M M M M M M I M M M g094 IKARGITGICRSΝAATTSGFSFLTAVEATTTSAPLTCSAVWPGX 70 80 90 100 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 325>: a094.seq 1 ATGTATTCGC CTTTGCCCAA GCGGGCGTTG GTGCCTGCGG CGTTGAGTTT 51 GCCGCCGATA ACGAAAGTCG GGTCAAGTCC TGCCGCGCCG AGGATGGAGG 101 CGGTCAGGCT GGTGGTCGTG GTTTTGCCGT GCGTGCCGGC AATGGCGATG 151 CCGTCACGGA AGCGCATCAA CTCCGCCAAC ATCAGGGCGC GCGGAATAAC 201 GGGAATTTGC TGCTCCAACG CAGCGACAAC TTCGGGATTT TCTTTTTTGA 251 CGGCGGTAGA GGTAACGACG ACATCCGCAC CGTTAACGTG TTCTGCGGTA 301 TGGCCGGGAT AA This corresponds to the amino acid sequence <SEQ ID 326; ORF 094. a>: a094.pep 1 MYSPLPKRAL VPAALSLPPI TKVGSSPAAP RMEAVRLVW VLPCVPAMAM 51 PSRKRINΞAN IRARGITGIC CSNAATTΞGF SFLTAVEVTT TSAPLTCSAV 101 WPG* m094/a094 100.0%) identity over a 103 aa overlap 10 20 30 40 50 60 m094.pep MYSPLPKRALVPAALSLPPITKVGSSPAAPRMEAVRLVWVLPCVPAMAMPSRKRINSAN I II II I I II II I II II II II I II II II II II II II II I I I II I II II I II I II II I II II a094 MYSPLPKRALVPAALSLPPITKVGSSPAAPRMEAVRLWWLPCVPAMAMPSRKRINSAN 10 20 30 40 50 60 70 80 90 100 mO 94. pep IRARGITGICCSNAATTSGFSFLTAVEVTTTSAPLTCSAVWPGX II I II II II I I II II I II I II I I II II I II II I II II II I I II I a094 IRARGITGICCSNAATTSGFSFLTAVEVTTTSAPLTCSAVWPGX 70 80 90 100 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 327>: g095. seq 1 ATGTCCTTTC ATTTGAACAT GGACGGTGAA TTTCATTTGC GCGCCGACGT 51 TTTCGATGTC GGTGGCGTAG ATGTCGGCGG TATCGTCCAA ACCGTAAGTA 101 GCATAAGGTT TGCTCACTTT GGGCAAAATC GCGCGGACGT GTTCGCTGTC 151 AACACACAAA AAGGCTTTGC CGTAGAAGGG CATACGGTGG ATGAAATCGA 201 TAAACGCCTG ATGCAGTTTT TCGACGCTGT GCCCGTAGGT ATCCATATGG 251 TCTTCGTCGA TATTGGTAAC GACGGACATA ATCGGGGTCA GTGTAGGAAA 301 GAGGCATCGG ATCGTCGGCT TCGGCAACGA TGTATTCGCC TTTGCCCAAG 351 CGGGCGTTGG TGCCTGCGGC GTTGA This corresponds to the amino acid sequence <SEQ ID 328; ORF 095. ng>: g095.pep 1 MSFHLΝMDGE FHLRADVFDV GGVDVGGIVQ TVSSIRFAHF GQΝRADVFAV 51 ΝTQKGFAVEG HTVDEIDKRL MQFFDAVPVG IHMVFVDIGΝ DGHΝRGQCRK 101 EASDRRLRQR CIRLCPSGRW CLRR* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 329>: m095. seq 1 ATGTCCTTTC ATTTGAACAT GGACGGTGAA TTTCATTTGC GCGCCGACGT 51 TTTCGATGTC GGTGGCGTAG ATGTCGGCGG TATCGTCCAA ACCGTAAGTA 101 GCATAAGGTT TGCTCACTTT GGGCAAAATC GCGCGGACGT GTTCGCTGTC 151 AATACACAAA AAGGCTTTGC CGTAGAAGGG CATACGGTGG ATGAAATCGA 201 TAAACGCCTG ATGCAGTTTT TCGACGCTGT GCCCGTAGGT ATCCATATGG 251 TCTTCGTCGA TATTGGTAAC GACGGACATA ATCGGTGTCA GTGCAGAAAG 301 GATGCATCCG ACCGTCGGCT TCGGCAACGA TGTATTCGCC TTTGCCCAAG 351 CGGGCGTTAG TGCCTGCGGC GTTGA This corresponds to the amino acid sequence <SEQ ID 330; ORF 095>: m095.pep 1 MSFHLΝMDGE FHLRADVFDV GGVDVGGIVQ TVSSIRFAHF GQΝRADVFAV 51 ΝTQKGFAVEG HTVDEIDKRL MQFFDAVPVG IHMVFVDIGΝ DGHΝRCQCRK 101 DASDRRLRQR CIRLCPSGRX CLRR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 095 shows 97.6%> identity over a 124 aa overlap with a predicted ORF (ORF 095. ng) from N gonorrhoeae: 10 20 30 40 50 60 m095.pep MSFHLΝMDGEFHLRADVFDVGGVDVGGIVQTVSSIRFAHFGQΝRADVFAVΝTQKGFAVEG g095 MSFHLNMDGEFHLRADVFDVGGVDVGGIVQTVSSIRFAHFGQNRADVFAVNTQKGFAVEG 10 20 30 40 50 60 70 80 90 100 110 120 m095.pep HTVDEIDKRLMQFFDAVPVGIHMVFVDIGNDGHNRCQCRKDASDRRLRQRCIRLCPSGRX M M M M M M M M M M I M M M M M M M 1 1 1 1 M 1 1 1 1 1 1 1 1 I I I I I 1 1 1 1 gO95 HTVDEIDKRLMQFFDAVPVGIHMVFVDIGNDGHNRGQCRKEASDRRLRQRCIRLCPSGRW 70 80 90 100 110 120 m095.pep CLRRX Mill gO95 CLRRX The following partial DNA sequence was identified in N meningitidis <SEQ ID 331>: a095. seq 1 ATGTCCTTTC ATTTGAACAT GGACGGTGAA TTTCATTTGC GCGCCGACGT 51 TTTCGATGTC GGTGGCGTAG ATGTCGGCGG TATCGTCCAA ACCGTAAGTA 101 GCATAAGGTT TGCTCACTTT GGGCAAAATC GCGCGGACGT GTTCGCTGTC 151 AATACACAAA AAGGCTTTGC CGTAGAAGGG CATACGGTGG ATGAAATCGA 201 TAAACGCCTG ATGCAGCTTC TCAACACTGT GCCCGTAGGT ATCCATATGG 251 TCTTCGTCGA TATTGGTAAC GACGGACATA ATCGGTGTCA GTGCAGAAAG 301 GATGCATCCG ACCGTCGGCT TCGGCAACGA TGTATTCGCC TTTGCCCAAG 351 CGGGCGTTGG TGCCTGCGGC GTTGA This corresponds to the amino acid sequence <SEQ ID 332; ORF 095. a>: a095.pep 1 MSFHLΝMDGE FHLRADVFDV GGVDVGGIVQ TVSSIRFAHF GQΝRADVFAV 51 ΝTQKGFAVEG HTVDEIDKRL MQLLΝTVPVG IHMVFVDIGΝ DGHΝRCQCRK 101 DASDRRLRQR CIRLCPSGRW CLRR* m095/a095 96.0% identity in 124 aa overlap 10 20 30 40 50 60 m095. pep MSFHLΝMDGEFHLRADVFDVGGVDVGGIVQTVSSIRFAHFGQΝRADVFAVΝTQKGFAVEG II I I I I II II I I II II II II II I II II I II II II II I II II II II II II II I I II II II I a095 MSFHLΝMDGEFHLRADVFDVGGVDVGGIVQTVSSIRFAHFGQΝRADVFAVΝTQKGFAVEG 10 20 30 40 50 60 70 80 90 100 110 120 m095.pep HTVDEIDKRLMQFFDAVPVGIHMVFVDIGΝDGHΝRCQCRKDASDRRLRQRCIRLCPSGRX II II II II II I I :::: I I II II II II I II II I II I II II I II II II I II I II II II II I a095 HTVDEIDKRLMQLLΝTVPVGIHMVFVDIGΝDGHΝRCQCRKDASDRRLRQRCIRLCPSGRW 70 80 90 100 110 120 m095.pep CLRRX II II I a095 CLRRX The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 333>: g096. seq 1 ATGGCCGGTC ATACCGGGCA GGGTGTTGAT TTCCAACAGA TAGAGTTTGC 51 CGTCGGTATC TTTGAGGAAA TCGACGCGCA CGCAGCCTTC CGCACCGATT 101 GCCTGTGCGC CGCGAACCGC CAGTTCGCGC ATCAGGCTTT CTTCGGCTTC 151 GGTCAAATCT TCCGAAGGAC ATTGAtaaat ggtgTCGTCT CGGttgtaCt 201 tggcttcgta gTCGTAAAAC TCGGTTGCGG GGATGATGTG TATGCCGGGC 251 AGCCCTTTGC CGTTCAGGAC GGGGCAGGAA TATTCGCCGC CGCCGATAAA 301 AcgtTcggca atgaTTtcgc ccctgAAGGT GttTCAattc ttcgtaAACG 351 CTTTTCAGAC ggccttTTTC TTTGA This corresponds to the amino acid sequence <SEQ ID 334; ORF 096.ng>: g096.pep 1 MAGHTGQGVD FQQIEFAVGI FEEIDAHAAF RTDCLCAANR QFAHQAFFGF 51 GQIFRRTLIN GWSWLGFV WKLGCGDDV YAGQPFAVQD GAGIFAAADK 101 TFGNDFAPEG VSILRKRFSD GLFL* The following partial DNA sequence was identified in N meningitidis <SEQ ID 335>: m096. seq 1 ATGGCTCGTC ATACCGGGCA GGGTGTTGAT TTCCAACAGA TAGAGTTTGC 51 CGTCGGTATC TTTGAGGAAA TCGACGCGCA CGCAGCCTTC CGCACCGATT 101 GCCTGCGCGC CGCGAACCGC CAGTTCGCGC ATCAGGCTTT CTTCGGCTTC 151 GGTCAAATCT TCCGAAGGAC ATTGATAAAT GGTGTCGTCG CGGTTGTACT 201 TGGCTTCGTA GTCGTAAAAC TCGGTTGCGG GAATGATGTG TATGCCGGGC 251 AGCCCTTTGC CGTTCAGGAC GGGGCAGGAA TATTCGCCGC CGCCGATAAA 301 ACGTTCGGCA ATGATTTCGC CC.TGAAGGT GTTTCAATTC TTCGTAAACG 351 CTTTTCAGAC GGCCTTTTCC TTTGA This corresponds to the amino acid sequence <SEQ ID 336; ORF 096>: m096.pep 1 MARHTGQGVD FQQIEFAVGI FEEIDAHAAF RTDCLRAAΝR QFAHQAFFGF 51 GQIFRRTLIN GVVAVVLGFV WKLGCGNDV YAGQPFAVQD GAGIFAAADK 101 TFGNDFAXEG VSILRKRFSD GLFL* m096/g096 96.0% identity in 124 aa overlap 10 20 30 40 50 60 m096. pep MARHTGQGVDFQQIEFAVGIFEEIDAHAAFRTDCLRAANRQFAHQAFFGFGQIFRRTLIN II I II II II I I II II I II I II II II II I II I II I I I II I II II I II I I II I II I I II I g096 MAGHTGQGVDFQQIEFAVGIFEEIDAHAAFRTDCLCAANRQFAHQAFFGFGQIFRRTLIN 10 20 30 40 50 60 70 80 90 100 110 120 m096. pep GWAWLGFVWKLGCGNDVYAGQPFAVQDGAGIFAAADKTFGNDFAXEGVSILRKRFSD I II : I II II II I II I II M I II II II II II II II I I II II I II II II I II I II I II I II g096 GWSWLGFVWKLGCGDDVYAGQPFAVQDGAGIFAAADKTFGNDFAPEGVSILRKRFSD 70 80 90 100 110 120 m096.pep GLFLX II II I g096 GLFLX The following partial DNA sequence was identified in N meningitidis <SEQ ID 337>: a096. seq 1 ATGGCCGGTC ATACCGGGCA GGGTGTTGAT TTCCAACAGA TAGAGTTTGC 51 CGTCGGTATC TTTGAGGAAA TCGACGCGCA CGCAGCCTTC CGCACCGATT 101 GCCTGCGCGC CGCGAACCGC CAGTTCGCGC ATCAGGCTTT CTTCGGCTTC 151 GGTCAGATCT TCCGAAGGAC ATTGATAAAT GGTGTCGTTG CGGTTGTACT 201 TGGCTTCGTA GTCATAAAAC TCGGTCGCGG GGATGATGTG TATGCCGGGC 251 AGGCCTTTGC CGTTCAACAC AGGGCAGGAA TATTCGCCGC CGCCGATAAA 301 CCGTTCGGCA ATGATTTCGC CCT . GAAAGT GTTTCAATTC TTCGTAAACG 351 CTTTTCAGAC GGCCTTTTCC TTTGA This corresponds to the amino acid sequence <SEQ ID 338; ORF 096.ng>: a096.pep 1 MAGHTGQGVD FQQIEFAVGI FEEIDAHAAF RTDCLRAAΝR QFAHQAFFGF 51 GQIFRRTLIN GVVAVVLGFV VIKLGRGDDV YAGQAFAVQH RAGIFAAADK 101 PFGNDFAXES VSILRKRFSD GLFL* m0 96 /a096 92 . 7 % identity in 124 aa overlap 10 20 30 40 50 60 m096 . pep MARHTGQGVDFQQIEFAVGIFEEIDAHAAFRTDCLRAANRQFAHQAFFGFGQIFRRTLIN I I I I I I I I I I I I I I I I I I M M M I M M I I M I M M M M M M M I I M I M M I I a096 MAGHTGQGVDFQQIEFAVGIFEEIDAHAAFRTDCLRAANRQFAHQAFFGFGQI FRRTLIN 10 20 30 40 50 60 70 80 90 100 110 120 m096 . pep GVVAWLGFVWKLGCGNDVYAGQPFAVQDGAGIFAAADKTFGNDFAXEGVSILRKRFSD I I I I I I I I I I I : I I I M I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I a096 GWAWLGFWIKLGRGDDVYAGQAFAVQHRAGIFAAADKPFGNDFAXESVSILRKRFSD 70 80 90 100 110 120 m096 . pep GLFLX I I I I I a096 GLFLX The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 339>: g097.seq 1 ATGGATATTT CAAAACAAAC ATTGCTGGAT AGGGTTTTTA ACCTGAAGGC 51 AAACGGTACG ACGGTACGTA CCGAGTTGAT GGCGGGTTTG ACGACCTTTT 101 TGACGATGTG CTACATCGTT ATCGTCAATC CCCTGATTTT GGGCGAGACC 151 GGAATGGATA TGGGGGCGGT ATTCGTCGCT ACCTGTATCG CATCCGCCAT 201 CGGCTGTTTT GTCATGGGTT TTATCGGCAA CTATCCGATT GCGCTTGCCC 251 CGGGGATGGG GCTGAATGCC TATTTCACCT TTGCCGTCGT TAAGGGTATG 301 GGCGTGCCTT GGCAGGTGGC GTTGGGTGCG GTGTTCATTT CCGGTCTGAT 351 TTTCATCCTG TTCAGCTTTT TTAAAGTCAG GGAAATGCTG GTCAACGCAC 401 TGCCTATGGG TTTGAAAATG TCGATTGCCG CCGGTATCGG TTTGTTTTTG 451 GCACTGATTT CCCTGAAAGG CGCAGGCATT ATCGTTGCCA ATCCGGCAAC 501 CTTGGTCGGC TTGGGCGATA TTCATCAGCC CAGCGCACTG TTGGCATTGT 551 TCGGTTTTGT CATGGTGGTC GTATTGGGGT ATTTCCGCGT TCAAGGCGCA 601 ATCATCATCA CCATTCTGAC GATTACCGTC ATTGCCAGCC TGATGGGTTT 651 GAACGAGTTT CACGGCGTGG TCGGCGAAGT ACCGGGCATT GCGCCGACCT 701 TTATGCAGAT GGATTTTAAA GGTCTGTTTA CCGTCAGCAT GGTCAGCGTG 751 ATTTTCGTCT TCTTCTTGGT CGATTTGTTC GACAGTACCG GAACGCTGGT 801 CGGCGTATCC CACCGTGCCG GACTGCTGGT GGACGGTAAG CTGCCCCGCC 851 TGAAACGCGC ACTGCTTGCA GACTCTACCG CCATTGTGGC AGGTGCGGCT 901 TTGGGTACTT CTTCAACCAC GCCTTATGTG GAAAGCGCGG CGGGCGTATC 951 GGCAGGCGGA CGGACCGGCC TGACGGCGGT TACCGTCGGC GTATTGATGC 1001 TGGCGTGTCT GATGTTCTCC CCATTGGCGA AAAGTGTTCC GGTATTTGCC 1051 ACCGCGCCCG CACTGCTTTA TGTCGGCACG CAGATGCTCC GCAGTGCGAG 1101 GGACATTGAT TGGGACGATA TGACTGAAGC CGCGCCCGCG TTCCTGACCA 1151 TTGTCTTCAT GCCGTTTACC TATTCGATTG CAGACGGCAT CGCCTTCGGC 1201 TTCATCAGCT ATGCCGTGGT CAAACTTTTG TGTCGCCGGA CTGGGGACGT 1251 GCCGCCTATG GTATGGGTTG TTGCCGTATT GTGGGCATTG AAATTCTGGT 1301 ATTTGGGCTG A This corresponds to the amino acid sequence <SEQ ID 340; ORF 097.ng>: g097.pep 1 MDISKQTLLD RVFΝLKAΝGT TVRTELMAGL TTFLTMCYIV IVΝPLILGET 51 GMDMGAVFVA TCIASAIGCF VMGFIGΝYPI ALAPGMGLΝA YFTFAWKGM 101 GVPWQVALGA VFISGLIFIL FSFFKVREML VΝALPMGLKM SIAAGIGLFL 151 ALISLKGAGI IVAΝPATLVG LGDIHQPSAL LALFGFVMW VLGYFRVQGA 201 IIITILTITV IASLMGLΝEF HGWGEVPGI APTFMQMDFK GLFTVSMVSV 251 IFVFFLVDLF DSTGTLVGVS HRAGLLVDGK LPRLKRALLA DSTAIVAGAA 301 LGTSSTTPYV ESAAGVSAGG RTGLTAVTVG VLMLACLMFS PLAKSVPVFA 351 TAPALLYVGT QMLRSARDID WDDMTEAAPA FLTIVFMPFT YSIADGIAFG 401 FISYAWKLL CRRTGDVPPM VW AVLWAL KFWYLG* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 341 >: m097.seq 1 ATGGACACTT CAAAACAAAC ACTGTTGGAC GGGATTTTTA AGCTGAAGGC 51 AAACGGTACk ACGGTGCGTA CCGAGTTGAT GGCGGGTTTG ACAACTTTTT 101 TGACGATGTG CTACATCGTT ATCGTCAACC CTCyGATTTT GGGCGAGACC 151 GGCATGGATA TGGGGGCGGT ATTCGTCGCT ACCTGTATCG CGTCTGCCAT 201 CGGCTGTTTT GTTATGGGTT TTGTCGGCAA CTATCCGATT GCACTCGCAC 251 CGGGGATGGG GCTGAATGCC TATTTCACCT TTGCCGTCGT TAAGGGTATG 301 GGCGTGCCTT GGCAGGTTGC GTTGGGTGCG GTGTTCATCT CCGGTCTGAT 351 TTTTATCCTG TTCAGCTTTT TTAAAGTCAG GGAAATGCTG GTCAACGCAC 401 TGCCTATGGG TTTGAAAATG TCGATTGCTG CCGGTATCGG TTTGTTTTTG 451 GCACTGATTT CCCTGAAAGG CGCAGGCATT ATCGTTGCCA ATCCGGCAAC 501 CTTGGTCGGT TTGGGCGATA TTCATCAGCC GTCCGCGTTG TTGGCATTGT 551 TCGGTTTTGC TATGGTGGTC GTATTGGGAC ATTTCCGCGT TCAAGGCGCA 601 ATCATCATCA CCATCTTGAC CATTACCGTC ATTGCCAGCC TGATGGGTTT 651 GAATGAATTT CACGGCATCA TCGGCGAAGT ACCGAGCATT GCGCCGACTT 701 TTATGCAGAT GGATTTTGAA GGCCTGTTTA CCGTCAGCAT GGTCAGTGTG 751 ATTTTCGTCT TCTTCTTGGT CGATCTATTT GACAGTACCG GAACGCTGGT 801 CGGCATATCC CACCGTGCCG GGCTGCTGGT GGACGGTAAG CTGCCCCGCC 851 TGAAACGCGC ACTGCTTGCA GACTCTACCG CCATTGTGGC AGGTGCGGCT 901 TTGGGTACTT CTTCCACCAC GCCTTATGTG GAAAGCGCGG CGGGCGTATC 951 GGCAGGCGGA CGGACCGGCC TGACGGCGGT TACCGTCGGC GTATTGATGC 1001 TCGCCTGCCT GATGTTTTCA CCTTTGGCGA AAAGTGTTCC CGCTTTTGCC 1051 ACCGCGCCCG CCCTGCTTTA TGTCGGCACG CAGATGCTCC GCAGTGCGAG 1101 GGATATTGAT TGGGACGATA TGACGGAAGC CGCACCTGCG TTCCTGACCA 1151 TTGTTTTCAT GCCGTTTACT TATTCGATTG CAGACGGCAT CGCTTTCGGC 1201 TTCATCAGTT ATGCCGTGGT TAAACTTTTA TGCCGCCGCA CCAAAGACGT 1251 TCCGCCTATG GTATGGATTG TTGCCGTATT GTGGGCACTG AAATTCTGGT 1301 ATTTGGGCTG A This corresponds to the amino acid sequence <SEQ ID 342; ORF 097>: m097.pep 1 MDTSKQTLLD GIFKLKAΝGT TVRTELMAGL TTFLTMCYIV IVΝPXILGET 51 GMDMGAVFVA TCIASAIGCF VMGFVGΝYPI ALAPGMGLΝA YFTFAWKGM 101 GVPWQVALGA VFISGLIFIL FSFFKVREML VΝALPMGLKM SIAAGIGLFL 151 ALISLKGAGI IVAΝPATLVG LGDIHQPSAL LALFGFAMW VLGHFRVQGA 201 IIITILTITV IASLMGLΝEF HGIIGEVPSI APTFMQMDFE GLFTVSMVSV 251 IFVFFLVDLF DSTGTLVGIS HRAGLLVDGK LPRLKRALLA DSTAIVAGAA 301 LGTSSTTPYV ESAAGVSAGG RTGLTAVTVG VLMLACLMFS PLAKSVPAFA 351 TAPALLYVGT QMLRSARDID WDDMTEAAPA FLTIVFMPFT YSIADGIAFG 401 FISYAWKLL CRRTKDVPPM VWIVAVLWAL KFWYLG* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 097 shows 96.3% identity over a 436 aa overlap with a predicted ORF (ORF 097.ng) from N gonorrhoeae: m097/g097 10 20 30 40 50 60 mO97. pep MDTSKQTLLDGIFKLKAΝGTTVRTELMAGLTTFLTMCYIVIVΝPXILGETGMDMGAVFVA I I l l l l l l l M M M M M I M M M M M M M M M M M l l l l l l l l l l l l l l l g097 MDISKQTLLDRVFΝLKAΝGTTVRTELMAGLTTFLTMCYIVIVΝPLILGETGMDMGAVFVA 10 20 30 40 50 60 70 80 90 100 110 120 mO 97. pep TCIASAIGCFVMGFVGΝYPIALAPGMGLΝAYFTFAWKGMGVPWQVALGAVFISGLIFIL MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g097 TCIASAIGCFVMGFIGΝYPIALAPGMGLΝAYFTFAWKGMGVPWQVALGAVFISGLIFIL 70 80 90 100 110 120 130 140 150 160 170 180 m097.pep FSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGIIVANPATLVGLGDIHQPSAL MMMMMMMMMMIMMMMMMMMMMMMMMMMIMMM g097 FSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGIIVANPATLVGLGDIHQPSAL 130 140 150 160 170 180 190 200 210 220 230 240 m097 . pep LALFGFAMVWLGHFRVQGAI IITILTITVIASLMGLNEFHGIIGEVPSIAPTFMQMDFE MMMMMMIMMMMMMMMMMMMMMMMMIMMMMMM gO97 LALFGFVMVWLGYFRVQGAIIITILTITVIASLMGLNEFHGWGEVPGIAPTFMQMDFK 190 200 210 220 230 240 250 260 270 280 290 300 m097.pep GLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLLVDGKLPRLKRALLADSTAIVAGAA MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g097 GLFTVSMVSVIFVFFLVDLFDSTGTLVGVSHRAGLLVDGKLPRLKRALLADSTAIVAGAA 250 260 270 280 290 300 310 320 330 340 350 360 mO97. pep LGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLMLACLMFSPLAKSVPAFATAPALLYVGT 11111111111111111111111111111111111111111111111 = 111111111111 g097 LGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLMLACLMFSPLAKSVPVFATAPALLYVGT 310 320 330 340 350 360 370 380 390 400 410 420 m097. pep QMLRSARDIDWDDMTEAAPAFLTIVFMPFTYSIADGIAFGFISYAWKLLCRRTKDVPPM MMMMMMMMMMMMMMMMMMMMMMMMMMM Mill g097 QMLRSARDIDWDDMTEAAPAFLTIVFMPFTYSIADGIAFGFISYAWKLLCRRTGDVPPM 370 380 390 400 410 420 430 mO 97 . pep VWI VAVLWALKFWYLGX I I : I I I I I I I I I I I I I I g097 VWWAVLWALKFWYLGX 430 The following partial DNA sequence was identified in N meningitidis <SEQ ID 343> a097.seq 1 ATGGACACTT CAAAACAAAC ACTGTTGGAC GGGATTTTTA AGCTGAAGGC 51 AAACGGTACG ACGGTGCGTA CCGAGTTGAT GGCGGGTTTG ACAACTTTTT 101 TGACGATGTG CTACATCGTT ATCGTCAACC CTCTGATTTT GGGCGAGACC 151 GGCATGGATA TGGGGGCGGT ATTCGTCGCT ACCTGTATCG CGTCTGCCAT 201 CGGCTGTTTT GTTATGGGTT TTGTCGGCAA CTATCCGATT GCACTCGCAC 251 CGGGGATGGG GCTGAATGCC TATTTCACCT TTGCCGTCGT TAAGGGTATG 301 GGCGTGCCTT GGCAGGTTGC GTTGGGTGCG GTGTTCATCT CCGGTCTGAT 351 TTTCATCCTG TTCAGCTTTT TTAAAGTCAG GGAAATGCTG GTCAACGCAC 401 TGCCTATGGG TTTGAAAATG TCGATTGCTG CCGGTATCGG TTTGTTTTTG 451 GCACTGATTT CCCTGAAAGG CGCAGGCATT ATCGTTGCCA ATCCGGCAAC 501 CTTGGTCGGC TTGGGCGATA TTCATCAGCC GTCCGCGTTG TTGGCACTGT 551 TCGGTTTTGC CATGGTGGTC GTATTGGGAC ATTTCCGCGT TCAAGGCGCA 601 ATCATCATCA CCATTTTGAC GATTACCGTC ATTGCCAGCC TGATGGGTTT 651 GAACGAATTT CACGGCATCA TCGGCGAAGT GCCGAGCATT GCGCCGACTT 701 TTATGCAGAT GGATTTTAAA GGGTTGTTTA CCGTCAGCAT GGTCAGCGTG 751 ATTTTCGTCT TTTTCCTAGT CGATCTGTTC GACAGTACCG GAACACTGGT 801 CGGTGTATCG CATCGTGCCG GACTGCTGGT GGACGGTAAG CTGCCCCGCC 851 TGAAACGCGC ACTGCTTGCA GACTCTACCG CTATTGTGGC AGGTGCGGCT 901 TTGGGTACTT CTTCAACCAC GCCTTATGTG GAAAGTGCGG CGGGCGTATC 951 GGCAGGCGGG CGGACAGGTC TGACGGCGGT TACCGTCGGC GTATTGATGC 1001 TCGCCTGCCT GATGTTTTCA CCTTTGGCGA AAAGTGTTCC CGCTTTTGCC 1051 ACCGCGCCCG CCCTGCTTTA TGTCGGCACG CAGATGCTCC GCAGTGCGAG 1101 GGACATCGAT TGGGACGATA TGACGGAAGC CGCACCCGCA TTCCTGACCA 1151 TTGTCTTCAT GCCGTTTACC TATTCGATTG CAGACGGCAT CGCTTTCGGC 1201 TTCATCAGTT ATGCCGTGGT TAAACTTTTA TGCCGCCGCA CCAAAGACGT 1251 TCCGCCTATG GTATGGATTG TTGCCGTATT GTGGGCACTG AAATTCTGGT 1301 ATTTGGGCTG A This corresponds to the amino acid sequence <SEQ ID 344; ORF 097. a>: a097.pep 1 MDTSKQTLLD GIFKLKANGT TVRTELMAGL TTFLTMCYIV IVNPLILGET 51 GMDMGAVFVA TCIASAIGCF VMGFVGNYPI ALAPGMGLNA YFTFAWKGM 101 GVPWQVALGA VFISGLIFIL FSFFKVREML VNALPMGLKM SIAAGIGLFL 151 ALISLKGAGI IVANPATLVG LGDIHQPSAL LALFGFAMW VLGHFRVQGA 201 IIITILTITV IASLMGLNEF HGIIGEVPSI APTFMQMDFK GLFTVSMVSV 251 IFVFFLVDLF DSTGTLVGVS HRAGLLVDGK LPRLKRALLA DSTAIVAGAA 301 LGTSSTTPYV ESAAGVSAGG RTGLTAVTVG VLMLACLMFS PLAKSVPAFA 351 TAPALLYVGT QMLRSARDID WDDMTEAAPA FLTIVFMPFT YSIADGIAFG 401 FISYAWKLL CRRTKDVPPM VWIVAVLWAL KFWYLG* m097/a097 99.3% identity in 436 aa overlap 10 20 30 40 50 60 m097. pep MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNPXILGETGMDMGAVFVA I II I II II II II I II II II I II II I II II I II I I II II II I II I lllllllllllllll a097 MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNPLILGETGMDMGAVFVA 10 20 30 40 50 60 70 80 90 100 110 120 m097. pep TCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGVPWQVALGAVFISGLIFIL I I II I II II II II I II I II II II I II II II I II II I I II I II II II II II I II I II II II a097 TCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAWKGMGVPWQVALGAVFISGLIFIL 70 80 90 100 110 120 130 140 150 160 170 180 m097.pep FSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGIIVANPATLVGLGDIHQPSAL I II II I I I II II II II II II I II I II II II II I I II II II I II II II I I I II II I I I II I a097 FSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGIIVANPATLVGLGDIHQPSAL 130 140 150 160 170 180 190 200 210 220 230 240 m097.pep LALFGFAMVWLGHFRVQGAIIITILTITVIASLMGLNEFHGIIGEVPSIAPTFMQMDFE II II I II II I I I II I II II I II I II II II II II II II II II I II II I II I I II I II I I I : a097 LALFGFAMVWLGHFRVQGAIIITILTITVIASLMGLNEFHGIIGEVPSIAPTFMQMDFK 190 200 210 220 230 240 250 260 270 280 290 300 m097. pep GLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLLVDGKLPRLKRALLADSTAIVAGAA I I I II II I I I II II II II I II II I II II : II I II II II II II I II II I I I II II I I II I I a097 GLFTVSMVSVIFVFFLVDLFDSTGTLVGVSHRAGLLVDGKLPRLKRALLADSTAIVAGAA 250 260 270 280 290 300 310 320 330 340 350 360 m097. pep LGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLMLACLMFSPLAKSVPAFATAPALLYVGT II I II II II I II II I I I II I I I I II II II II I II I I II II II I II II II I II I II I II II a097 LGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLMLACLMFSPLAKSVPAFATAPALLYVGT 310 320 330 340 350 360 370 380 390 400 410 420 m097.pep QMLRSARDIDWDDMTEAAPAFLTIVFMPFTYSIADGIAFGFISYAWKLLCRRTKDVPPM II I I II I I I I I II I I I I II II I II I II I I II II II II II I II I II II I I I II II I I I II I a097 QMLRSARDIDWDDMTEAAPAFLTIVFMPFTYSIADGIAFGFISYAWKLLCRRTKDVPPM 370 380 390 400 410 420 430 O 97. pep VWIVAVLWALKFWYLGX I II I II I I II II II II I a097 VWIVAVLWALKFWYLGX 430 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 345>: g098.seq 1 ATGACCGCCG ACGGTCTCTT CGTCGCTTTC AACTTCAATA CGTTTGCCGT 51 TGTGCGAATA TTGATACCAG TACAGCAGGA TGCTGCCCAG GCTGGCGATC 101 AGTTTGTCGG CGATGTCGCG CGCTTCGCTG TCGGGATGGC TTTCGCGTTC 151 GGGATGAACG CAGCCGAGCA TGGACACGCC GGTACGCATC ACGTCCATCG 201 GATGGGTATG TGCAGGCAGG CTTTCCAAAA CTTTAATCAC ACGGATAGGC 251 AGGCCGCGCA TGGATTTGAG CTTGGTTTTA TAAGCGGCCA GCTCGAATTT 301 GTTGGGCAGA TGGCCGTGAA TCAGCAAGTG TGCGACTTCT TCAAACTCGC 351 ATTTTTGTGC CAAATTAGAA TGTCGTAA This corresponds to the amino acid sequence <SEQ ID 346; ORF 098.ng>: g098.pep 1 MTADGLFVAF ΝFΝTFAWRI LIPVQQDAAQ AGDQFVGDVA RFAVGMAFAF 51 GMΝAAEHGHA GTHHVHRMGM CRQAFQΝFΝH TDRQAAHGFE LGFISGQLEF 101 VGQMAVΝQQV CDFFKLAFLC QIRMS* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 347>: m098. seq 1 ATGACCGCCG ATGGTCTCTT CGTCGCTTTC AACCTCAATG CGTTTGCCGT 51 TGTGCGAATA TTGATACCAG TACAAGAGGA TGCTGCCGAG GCTGGCGATC 101 AGTTTGTCGG CGATGTCGCG CGCTTCACTT TCCGGATGGC TTTCACGTTC 151 AGGATGAACG CAGCCCAGCA TGGATACGCC GGTACGCATT ACGTCCATCG 201 GATGGGTATG TGCAGGCAGG CTTTCCAAAA CTTTAATCAC ACGGATAGGC 251 AGGCCGCGCA TGGATTTGAG CTTGGTTTTA TAAGCGGCCA GCTCGAATTT 301 GTTGGGCAGA TGGCCGTGAA TCAGCAGGTG GGCGACTTCT TCAAACTCGC 351 ATTTTTGTGC CAAATCAGAA TGTCGTAA This corresponds to the amino acid sequence <SEQ ID 348; ORF 098>: m098.pep 1 MTADGLFVAF ΝLΝAFAWRI LIPVQEDAAE AGDQFVGDVA RFTFRMAFTF 51 RMΝAAQHGYA GTHYVHRMGM CRQAFQΝFΝH TDRQAAHGFE LGFISGQLEF 101 VGQMAVΝQQV GDFFKLAFLC QIRMS* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 098 shows 89.6% identity over a 125 aa overlap with a predicted ORF (ORF 098.ng) from N gonorrhoeae: m098/g098 10 20 30 40 50 60 m098.pep MTADGLFVAFΝLΝAFAWRILIPVQEDAAEAGDQFVGDVARFTFRMAFTFRMΝAAQHGYA M M M M M I M M M M M M M M I I M M I M M M M : I M M I I I I M I M g098 MTADGLFVAFΝFΝTFAWRILIPVQQDAAQAGDQFVGDVARFAVGMAFAFGMΝAAEHGHA 10 20 30 40 50 60 70 80 90 100 110 120 m098.pep GTHYVHRMGMCRQAFQΝFΝHTDRQAAHGFELGFISGQLEFVGQMAVΝQQVGDFFKLAFLC 111 :! 11 II 11111111 M 1111111 II 111111 M 11111 M 1111 M I g098 GTHHVHRMGMCRQAFQΝFΝHTDRQAAHGFELGFISGQLEFVGQMAVΝQQVCDFFKLAFLC 70 80 90 100 110 120 m098.pep QIRMSX g098 QIRMSX The following partial DΝA sequence was identified in N meningitidis <SEQ ID 349> : a098.seq 1 ATGACCGCCG ATGGTCTCTT CGTCGCTTTC AACCTCAATG CGTTTGCCGT 51 TGTGCGAATA TTGATACCAG TACAAGAGGA TGCTGCCGAG GCTGGCGATC 101 AGTTTGTCGG CGATGTCGCG CGCTTCACTT TCCGGATGGC TTTCACGTTC 151 AGGATGAACG CAGCCCAGCA TGGATACGCC GGTACGCATT ACGTCCATCG 201 GATGGGTATG TGCAGGCAGG CTTTCCAAAA CTTTAATCAC ACGGATAGGC 251 AGGCCGCGCA TGGATTTGAG CTTGGTTTTA TAAGCGGCCA GCTCGAATTT 301 GTTGGGCAGA TGGCCGTGAA TCAGCAGGTG GGCGACTTCT TCAAACTCGC 351 ATTTTTGTGC CAAATCAGAA TGTCGTAA This corresponds to the amino acid sequence <SEQ ID 350; ORF 098.a>: a098.pep 1 MTADGLFVAF NLNAFAWRI LIPVQEDAAE AGDQFVGDVA RFTFRMAFTF 51 RMNAAQHGYA GTHYVHRMGM CRQAFQNFNH TDRQAAHGFE LGFISGQLEF 101 VGQMAVNQQV GDFFKLAFLC QIRMS* m098/a098 100.0% identity in 125 aa overlap 10 20 30 40 50 60 m098. pep MTADGLFVAFNLNAFAWRILIPVQEDAAEAGDQFVGDVARFTFRMAFTFRMNAAQHGYA II II I II II II II I I I I II II I I II I II II I II I II I II II I II I II II II II I II II II a098 MTADGLFVAFNLNAFAWRILIPVQEDAAEAGDQFVGDVARFTFRMAFTFRMNAAQHGYA 10 20 30 40 50 60 70 80 90 100 110 120 m098. pep GTHYVHRMGMCRQAFQNFNHTDRQAAHGFELGFISGQLEFVGQMAVNQQVGDFFKLAFLC I I II II I II II II II II II II I I II I I II II II II I I II II II I II II I II I II II I II I a098 GTHYVHRMGMCRQAFQNFNHTDRQAAHGFELGFISGQLEFVGQMAVNQQVGDFFKLAFLC 70 80 90 100 110 120 m098.pep QIRMSX a098 QIRMSX The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 351>: g099.seq 1 ATGCTGGGAC GCGCGTCCAT GATGCGCCTG CCCGATATTG TCGGCGTGGA 51 GCTGACGGGC AAACGGCAGG CGGGCATTAC TGCCACAGAC ATCGTGTTGG 101 CACTGACCGA ATTCTTGCGT AAAGAGCGCG TGGTCGGGGC GTTTGTCGAA 151 TTTTTCGGCG AGGGCGCGAG AAGCCTGTCT ATCGGCGACC GCGCGACCAT 201 TTCCAACATG ACGCCGGAGT TCGGCGCGAC TGCCGCCATG TTCGCCATCG 251 ACGCGCAAAC TATTGATTAT TTGAAACTGA CCGGACGTGA CGACGCGCAG 301 GTGAAATTGG TGGAAACCTA CGCCAAAACC GCAGGCTTAT GGGCAGGTGG 351 CTTGAAAACC GCCGTTTATC CGCGCGTTTT GAAATTTGAT TTGAGCAGCG 401 TAACGCGCAA TATGGCAGGC CCGAGCAACC CGCACGCGCG TTTTGCCACC 451 GCCGATTTGG CGGCGAAAGG GCTGGCGAAG CCTTACGAAG AGCCTTCAGA 501 CGGCCAAATG CCTGACGGTG CAGTGATTAT TGCCGCGATT ACTTCGTGTA 551 CCAATACTTC CAACCCGCGC AACGTTGTCG CCGCCGCACT GTTGGCACGC 601 AATGCCAACC GCCTCGGCTT GAAACGCAAA CCTTGGGTGA AATCTTCGTT 651 TGCCCCGGGT TCAAAAGTAG CCGGAATCTA TTTGAAAGAA GCAGGCTTGT 701 TGCCCGAAAT GGAAAAACTC GGCTTCGGTA TCGTCGCCTT CGCATGTACC 751 ACCTGTAACG GCATGAgcgG CGCGCTcgaC CCGAAAATCC AACAAGAAAT 801 CATCGACCGC GAtttgtacg cCACCGCCGT ATTGTCAGGC AACCGCAACT 851 TCGACGGCCG TATCCATCCG TATGCGAAAC AGGCTTTCCT CGCTTCGCCT 901 CCTTTGGTCG TTGCCTACGC ATTGGCAGGT AGCATCCGTT TCGATATTGA 951 AAACGACGTA CTCGGCGTTG CAGACGGCCG CGAAATCCGC CTGAAAGATA 1001 TCTGGCCGAC AGACGAAGAA ATCGATGCCA TCGTTGCCGA ATATGTGAAA 1051 CCGCAACAAT TCCGCGACAT TTATATCCCG ATGTCCGACA CCGGCACAGC 1101 GCAAAAAGCA CCAAGCCCGC TGTACGACTG GCGACCGATG TCCACCTACA 1151 TCCGCCGTCC GCCCTATTGG GAAGGCGCAC TGGCAGGGGA ACGTACATTA 1201 AGAGGTATGC GTCCGCCGGC GATTTTGCCC GACAACATCA CCACCGACCA 1251 CATCTCgcca tCCAATGCGA TTTTGGCCGG cagTGCcgca ggtgaATATT 1301 TGGCGAAAAT GGGTTTGCCT GAAGAagaCT TCAACTCTTA CGCAACCCAC 1351 CGCGGCGACC ACTTGACCGC CCAACGCGCA ACCTTCGCCA ATCCGAAACT 1401 GTTTAACGAA ATGGTGAGAA ACGAAGACGG CAGCGTACGC CAAGGTtcgt 1451 tggcacgcgT tgaacCAGAA GGCCAAACCA TGCGCATGTG GGAAGCCATC 1501 GAAACCTATA TGAACCGCAA ACAGCCGCTT ATCATCATTG CCGGTGCGGA 1551 CTATGGTCAA GGCTCAAGCC GCGACTGGGC GGCGAAGGGC GTGCGGCTGG 1601 CGGGTGTGGA AGCCATCGCC GCCGAAGGTT TCGAGCGCAT CCACCGCACC 1651 AACCTCATCG GCATGGGCGT CTTGCCGCTG CAATTCAAAC CCGGCACCAA 1701 CCGCCATACC CTGCAACTGG ACGGTACGGA AACCTACGAC GTTGTCGGCG 1751 AACGCACACC GCGCTGCGGC CTGACCCTCG TGATTCACCG TAAAAACGGA 1801 GAAACCGTCG AAGTTCCGGT TACCTGCCGC CCCGATACCG CAGAAGAAGC 1851 ATTGGTATAT GAAGCCGGCG GCGTATTGCA ACGGTTTGCA CAGGACTTTT 1901 TGGAAGGGAA CGCGGCTTAG This corresponds to the amino acid sequence <SEQ ID 352; ORF 099.ng>: g099.pep 1 MLGRASMMRL PDIVGVELTG KRQAGITATD IVLALTEFLR KERWGAFVE 51 FFGEGARSLS IGDRATISNM TPEFGATAAM FAIDAQTIDY LKLTGRDDAQ 101 VKLVETYAKT AGLWAGGLKT AVYPRVLKFD LSSVTRNMAG PSNPHARFAT 151 ADLAAKGLAK PYEEPSDGQM PDGAVIIAAI TSCTNTSNPR NWAAALLAR 201 NANRLGLKRK PWVKSSFAPG SKVAGIYLKE AGLLPEMEKL GFGIVAFACT 251 TCNGMSGALD PKIQQEIIDR DLYATAVLSG NRNFDGRIHP YAKQAFLASP 301 PLWAYALAG SIRFDIENDV LGVADGREIR LKDIWPTDEE IDAIVAEYVK 351 PQQFRDIYIP MSDTGTAQKA PSPLYDWRPM STYIRRPPYW EGALAGERTL 401 RGMRPPAILP DNITTDHISP SNAILAGSAA GEYLAKMGLP EEDFNSYATH 451 RGDHLTAQRA TFANPKLFNE MVRNEDGSVR QGSLARVEPE GQTMRMWEAI 501 ETYMNRKQPL IIIAGADYGQ GSSRDWAAKG VRLAGVEAIA AEGFERIHRT 551 NLIGMGVLPL QFKPGTNRHT LQLDGTETYD WGERTPRCG LTLVIHRKNG 601 ETVEVPVTCR PDTAEEALVY EAGGVLQRFA QDFLEGNAA* The following partial DNA sequence was identified in N meningitidis <SEQ ID 353>: m099. seq 1 ATGCTGGGAC GCGCGTCCAT GATGCGCCTG CCCGATATTG TCGGCGTTGA 51 GCTGAACGGC AAACGGCAGG CGGGCATTAC GGCGACGGAT ATTGTGTTGG 101 CACTGACCGA GTTTCTGCGC AAAGAACGCG TGGTCGGGGC GTTTGTCGAA 151 TTCTTCGGCG AGGGCGCGAG AAGCCTGTCT ATCGGCGACC GCGCGACCAT 201 TTCCAACATG ACGCCGGAGT TCGGCGCGAC TGCCGCGATG TTCGCTATTG 251 ATGAGCAAAC CATTGATTAT TTGAAACTGA CCGGACGCGA CGACGCGCAG 301 GTGAAATTGG TGGAAACCTA CGCCAAAACC GCAGGCTTGT GGGCAGATGC 351 CTTGAAAACC GCCGTTTATC CTCGCGTTTT GAAATTTGAT TTGAGCAGCG 401 TAACGCGCAA TATGGCAGGC CCAAGTAACC CGCATGCCCG TTTTGCGACC 451 GCCGATTTGG CGGCGAAAGG GCTGGCGAAG CCTTACGAAG AGCCTTCGGA 501 CGGCCAAATG CCCGACGGCT CGGTCATCAT CGCCGCGATT ACCAGTTGCA 551 CCAACACTTC CAACCCGCGC AACGTTGTTG CCGCCGCGCT CTTGGCACGC 601 AATGCCAACC GTCTCGGCTT GAAACGCAAA CCTTGGGTGA AATCTTCGTT 651 TGCCCCGGGT TCAAAAGTAG CCGAAATCTA TTTGAAAGAA GCGGGCCTGT 701 TGCCCGAAAT GGAAAAACTC GGCTTCGGTA TCGTCGCCTT CGCCTGCACC 751 ACCTGCAACG GCATGAGTGG CGCGCTGGAT CCGAAAATCC AGAAAGAAAT 801 CATCGACCGC GATTTGTACG CCACCGCCGT ATTATCAGGC AACCGCAACT 851 TCGACGGCCG TATCCACCCG TATGCGAAAC AGGCTTTCCT CGCTTCGCCT 901 CCGTTGGTCG TTGCCTACGC GCTGGCAGGC AGTATCCGTT TCGATATTGA 951 AAACGACGTA CTCGGCGTTG CAGACGGCAA GGAAATCCGC CTGAAAGACA 1001 TTTGGCCTGC CGATGAAGAA ATCGATGCCG TCGTTGCCGA ATATGTGAAA 1051 CCGCAGCAGT TCCGCGATGT GTATGTACCG ATGTTCGACA CCGGCACAGC 1101 GCAAAAAGCA CCCAGTCCGC TGTACGATTG GCGTCCGATG TCCACCTACA 1151 TCCGCCGTCC GCCTTACTGG GAAGGCGCGC TGGCAGGGGA ACGCACATTA 1201 AGAGGTATGC GTCCGCTGGC GATTTTGCCC GACAACATCA CCACCGACCA 1251 CCTCTCGCCG TCCAATGCGA TTTTGGCCGT CAGTGCCGCA GGCGAGTATT 1301 TGGCGAAAAT GGGTTTGCCT GAAGAAGACT TCAACTCTTA CGCAACCCAC 1351 CGCGGCGACC ACTTGACCGC CCAACGCGCT ACCTTCGCCA ATCCGAAACT 1401 GTTTAACGAA ATGGTGAAAA ACGAAGACGG CAGCGTGCGC CAAGGCTCGT 1451 TCGCCCGCGT CGAACCCGAA GGCGAAACCA TGCGCATGTG GGAAGCCATC 1501 GAAACCTATA TGAACCGCAA ACAGCCGCTC ATCATCATTG CCGGTGCGGA 1551 CTATGGTCAA GGCTCAAGCC GCGACTGGGC TGCAAAAGGC GTACGCCTCG 1601 CCGGCGTAGA AGCGATTGTT GCCGAAGGCT TCGAGCGTAT CCACCGCACC 1651 AACCTTATCG GCATGGGCGT GTTGCCGCTG CAGTTCAAAC CCGACACCAA 1701 CCGCCATACC CTGCAACTGG ACGGTACGGA AACCTACGAC GTGGTCGGCG 1751 AACGCACACC GCGCTGCGAC CTGACCCTCG TGATTCACCG TAAAAACGGC 1801 GAAACCGTTG AAGTTCCCGT TACCTGCTGC CTCGATACTG CAGAAGAAGT 1851 ATTGGTATAT GAAGCCGGCG GCGTGTTGCA ACGGTTTGCA CAGGATTTTT 1901 TGGAAGGGAA CGCGGCTTAG This corresponds to the amino acid sequence <SEQ ID 354; ORF 099>: m099.pep 1 MLGRASMMRL PDIVGVELNG KRQAGITATD IVLALTEFLR KERWGAFVE 51 FFGEGARSLS IGDRATISNM TPEFGATAAM FAIDEQTIDY LKLTGRDDAQ 101 VKLVETYAKT AGLWADALKT AVYPRVLKFD LSSVTRNMAG PSNPHARFAT 151 ADLAAKGLAK PYEEPSDGQM PDGSVIIAAI TSCTNTSNPR NWAAALLAR 201 NANRLGLKRK PWVKSSFAPG SKVAEIYLKE AGLLPEMEKL GFGIVAFACT 251 TCNGMSGALD PKIQKEIIDR DLYATAVLSG NRNFDGRIHP YAKQAFLASP 301 PLWAYALAG SIRFDIENDV LGVADGKEIR LKDIWPADEE IDAWAEYVK 351 PQQFRDVYVP MFDTGTAQKA PSPLYDWRPM STYIRRPPYW EGALAGERTL 401 RGMRPLAILP DNITTDHLSP SNAILAVSAA GEYLAKMGLP EEDFNSYATH 451 RGDHLTAQRA TFANPKLFNE MVKNEDGSVR QGSFARVEPE GETMRMWEAI 501 ETYMNRKQPL IIIAGADYGQ GSSRDWAAKG VRLAGVEAIV AEGFERIHRT 551 NLIGMGVLPL QFKPDTNRHT LQLDGTETYD WGERTPRCD LTLVIHRKNG 601 ETVEVPVTCC LDTAEEVLVY EAGGVLQRFA QDFLEGNAA* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N. gonorrhoeae ORF 099 shows 96.2% identity over a 639 aa overlap with a predicted ORF (ORF 099.ng) from N. gonorrhoeae: m099/g099 10 20 30 40 50 60 m099.pep MLGRASMMRLPDIVGVELΝGKRQAGITATDIVLALTEFLRKERWGAFVEFFGEGARSLS MMMMIMMMMIMMMMMMMMMMMMMMMMMMMMM g099 MLGRASMMRLPDIVGVELTGKRQAGITATDIVLALTEFLRKERWGAFVEFFGEGARSLS 10 20 30 40 50 60 70 80 90 100 110 120 m099. pep IGDRATISΝMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVETYAKTAGLWADALKT MMMMMMMMMMMM I II II II I II II II I II 11111 II 111111 MM g099 IGDRATISΝMTPEFGATAAMFAIDAQTIDYLKLTGRDDAQVKLVETYAKTAGLWAGGLKT 70 80 90 100 110 120 130 140 150 160 170 180 O99. pep AVYPRVLKFDLSSVTRΝMAGPSΝPHARFATADLAAKGLAKPYEEPSDGQMPDGSVIIAAI MMMMMMMMMMMMMMMMMMIMMMMMMMMMIMM g099 AVYPRVLKFDLSSVTRΝMAGPSΝPHARFATADLAAKGLAKPYEEPSDGQMPDGAVI IAAI 130 140 150 160 170 180 190 200 210 220 230 240 m099. pep TSCTΝTSΝPRΝWAAALLARΝAΝRLGLKRKPWVKSSFAPGSKVAEIYLKEAGLLPEMEKL 11111111 i 111 I I ! I I M I i 11 ! 1111111111 ! 11 E 111 E 11 111 ! 11111111 E 11 g099 TSCTΝTSΝPRΝWAAALLARΝAΝRLGLKRKPWVKSSFAPGSKVAGIYLKEAGLLPEMEKL 190 200 210 220 230 240 250 260 270 280 290 300 m099. pep GFGIVAFACTTCΝGMSGALDPKIQKEIIDRDLYATAVLSGΝRΝFDGRIHPYAKQAFLASP MMMMIMMMMMMMIMMIMMMMMMMMMMMMMMMI g099 GFGIVAFACTTCΝGMSGALDPKI Q QEIIDRDLYATAVLSGΝRΝFDGRIHPYAKQAFLASP 250 260 270 280 290 300 310 320 330 340 350 360 m099.pep PLWAYALAGSIRFDIENDVLGVADGKEIRLKDIWPADEEIDAWAEYVKPQQFRDVYVP MMMMMMMMMMMMMMMMMMMMMIMMIMMMMMM g099 PLWAYALAGSIRFDIENDVLGVADGREIRLKDIWPTDEEIDAIVAEYVKPQQFRDIYIP 310 320 330 340 350 360 370 380 390 400 410 420 m099. pep MFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALAGERTLRGMRPLAILPDNITTDHLSP I MMMMMMMMMMMMMMMMMIMMMM MIMMMMMI gO 99 MSDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALAGERTLRGMRPPAILPDNITTDHISP 370 380 390 400 410 420 430 440 450 460 470 480 m099.pep SNAILAVSAAGEYLAKMGLPEEDFNSYATHRGDHLTAQRATFANPKLFNEMVKNEDGSVR MMMMMMMIMMMMMMMMMMMMMMMMMMM g099 SNAILAGSAAGEYLAKMGLPEEDFNSYATHRGDHLTAQRATFANPKLFNEMVRNEDGSVR 430 440 450 460 470 480 490 500 510 520 530 540 m099.pep QGSFARVEPEGETMRMWEAIETYMNRKQPLIIIAGADYGQGSSRDWAAKGVRLAGVEAIV MIMMMMMMMMMMMMMMMMMMMMMMMMIMMMM gO 99 QGSLARVEPEGQTMRMWEAIETYMNRKQPLI I IAGADYGQGSSRDWAAKGVRLAGVEAIA 490 500 510 520 530 540 550 560 570 580 590 600 m099. pep AEGFERIHRTNLIGMGVLPLQFKPDTNRHTLQLDGTETYDWGERTPRCDLTLVIHRKNG MMMMMMMMMMMM 11 II II II II I II I II I II II I II 111111 i I i I gO99 AEGFERIHRTNLIGMGVLPLQFKPGTNRHTLQLDGTETYDWGERTPRCGLTLVIHRKNG 550 560 570 580 590 600 610 620 630 640 mO 99. pep ETVEVPVTCCLDTAEEVLVYEAGGVLQRFAQDFLEGNAAX MIMMMMMMMMMMMMM g099 ETVEVPVTCRPDTAEEALVYEAGGVLQRFAQDFLEGNAAX 610 620 630 640 The following partial DNA sequence was identified in N meningitidis <SEQ ID 355> : a099.seq 1 ATGCTGGGAC GCGCGTCCAT GATGCGCCTG CCCGATATTG TCGGCGTTGA 51 GCTGAACGGC AAACGGAAGG CGGGCATTAC GGCGACGGAT ATTGTGTTGG 101 CACTGACCGA GTTTCTGCGC AAAGAACGCG TGGTCGGGGC GTTTGTCGAA 151 TTCTTCGGCG AGGGCGCGAG AAGCCTGTCT ATCGGCGACC GCGCGACCAT 201 TTCCAACATG ACGCCGGAGT TCGGCGCGAC TGCCGCGATG TTCGCTATTG 251 ATGAGCAAAC CATTGATTAT TTGAAACTGA CCGGACGCGA CGACGCGCAG 301 GTGAAATTGG TGGAAACCTA CGCCAAAACC GCAGGCTTGT GGGCAGATGC 351 CTTGAAAACC GCCGTTTATC CGCGCGTTTT GAAATTTGAT TTGAGCAGCG 401 TAACGCGCAA TATGGCAGGC CCGAGCAACC CGCACGCGCG TTTTGCGACC 451 GCCGATTTGG CCGGCAAAGG CTTGGCTAAA CCTTACGAAG AGCCTTCAGA 501 CGGCCAAATG CCTGACGGTG CAGTGATTAT TGCCGCGATT ACTTCCTGTA 551 CCAATACTTC CAATCCGCGC AACGTTGTCG CCGCCGCGCT GTTGGCACGC 601 AATGCCAACC GCCTCGGCTT GCAACGCAAA CCTTGGGTGA AATCTTCGTT 651 TGCCCCGGGT TCAAAAGTAG CCGAAATCTA TTTGAAAGAA GCAGATCTGC 701 TGCCCGAAAT GGAAAAACTC GGCTTCGGTA TCGTTGCCTT CGCATGTACC 751 ACCTGTAACG GCATGAGCGG CGCGCTGGAT CCGAAAATCC AGAAAGAAAT 801 CATCGACCGC GATTTGTACG CCACCGCCGT ATTGTCAGGC AACCGCAACT 851 TTGACGGCCG TATCCATCCG TATGCGAAAC AGGCTTTCCT CGCTTCGCCT 901 CCGTTGGTCG TTGCCTACGC GCTGGCAGGC AGCATCCGTT TCGATATTGA 951 AAACGACGTA CTCGGCGTTG CAGACGGCAA AGAAATCCGC CTGAAAGACA 1001 TTTGGCCTAC CGATGAAGAA ATCGATGCCA TCGTTGCCGA ATATGTGAAA 1051 CCGCAGCAAT TTCGCGACGT TTATATCCCG ATGTTCGACA CCGGCACAGC 1101 GCAAAAAGCA CCAAGCCCGC TGTACGACTG GCGTCCAATG TCTACCTATA 1151 TCCGCCGCCC ACCTTACTGG GAAGGCGCAC TGGCAGGGGA ACGCACATTA 1201 AGCGGTATGC GTCCGCTGGC GATTTTGCCC GACAACATCA CCACCGACCA 1251 TCTCTCGCCA TCCAATGCGA TTTTGGCAAG CAGTGCCGCA GGCGAATATT 1301 TGGCAAAAAT GGGTTTGCCT GAAGAAGACT TCAACTCTTA CGCAACCCAC 1351 CGTGGCGACC ACTTGACCGC CCAACGCGCA ACCTTCGCCA ATCCGAAACT 1401 GTTTAACGAA ATGGTGAGAA ACGAAGACGG CAGCGTACGC CAAGGTTCGC 1451 TGGCACGCGT TGAACCCGAA GGCCAAACCA TGCGCATGTG GGAAGCCATC 1501 GAAACCTATA TGAACCGCAA ACAGCCGCTC ATCATCATTG CCGGCGCGGA 1551 CTACGGTCAA GGCTCAAGCC GCGACTGGGC TGCAAAAGGC GTACGCCTCG 1601 CCGGCGTGGA AGCGATTGTT GCCGAAGGCT TCGAGCGTAT CCACCGCACC 1651 AACTTGATCG GTATGGGCGT GTTGCCGCTG CAGTTCAAAC CGGGTACCAA 1701 CCGCCACACC CTGCAACTGG ACGGTACGGA AACCTACGAC GTTGTCGGCG 1751 AACGCACACC GCGCTGCGAC CTGACCCTTG TGATTCACCG TAAAAACGGC 1801 GAGACCGTCG AAGTCCCCAT TACCTGCCGC CTCGATACCG CAGAAGAAGT 1851 GTTGGTATAT GAAGCCGGTG GCGTATTGCA ACGGTTTGCA CAGGATTTTT 1901 TGGAAGGGAA CGCGGCTTAG This corresponds to the amino acid sequence <SEQ ID 356; ORF 099. a>: a099.pep 1 MLGRASMMRL PDIVGVELNG KRKAGITATD IVLALTEFLR KERWGAFVE 51 FFGEGARSLS IGDRATISNM TPEFGATAAM FAIDEQTIDY LKLTGRDDAQ 101 VKLVETYAKT AGLWADALKT AVYPRVLKFD LSSVTRNMAG PSNPHARFAT 151 ADLAGKGLAK PYEEPSDGQM PDGAVIIAAI TSCTNTSNPR NWAAALLAR 201 NANRLGLQRK PWVKSSFAPG SKVAEIYLKE ADLLPEMEKL GFGIVAFACT 251 TCNGMSGALD PKIQKEIIDR DLYATAVLSG NRNFDGRIHP YAKQAFLASP 301 PLWAYALAG SIRFDIENDV LGVADGKEIR LKDIWPTDEE IDAIVAEYVK 351 PQQFRDVYIP MFDTGTAQKA PSPLYDWRPM STYIRRPPYW EGALAGERTL 401 SGMRPLAILP DNITTDHLSP SNAILASSAA GEYLAKMGLP EEDFNSYATH 451 RGDHLTAQRA TFANPKLFNE MVRNEDGSVR QGSLARVEPE GQTMRMWEAI 501 ETYMNRKQPL IIIAGADYGQ GSSRDWAAKG VRLAGVEAIV AEGFERIHRT 551 NLIGMGVLPL QFKPGTNRHT LQLDGTETYD WGERTPRCD LTLVIHRKNG 601 ETVEVPITCR LDTAEEVLVY EAGGVLQRFA QDFLEGNAA* 099/a099 97.5% identity in 639 aa overlap 10 20 30 40 50 60 mO 99. pep MLGRASMMRLPDIVGVELNGKRQAGITATDIVLALTEFLRKERWGAFVEFFGEGARSLS I I II I II II II I II I II II I II : II II II II II I II II II II II II II I II I II I II II I a099 MLGRASMMRLPDIVGVELNGKRKAGITATDIVLALTEFLRKERWGAFVEFFGEGARSLS 10 20 30 40 50 60 70 80 90 100 110 120 m099.pep IGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVETYAKTAGLWADALKT I II II I I II II I I II II II II I I I I I II I I II II II I I II II I I II II I II I I II II II I a099 IGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVETYAKTAGLWADALKT 70 80 90 100 110 120 130 140 150 160 170 180 m099.pep AVYPRVLKFDLSSVTRNMAGPSNPHARFATADLAAKGLAKPYEEPSDGQMPDGSVIIAAI I II II I II I II I I II II II II I I II II I II II II: II II II II II I II II II I: II II II a099 AVYPRVLKFDLSSVTRNMAGPSNPHARFATADLAGKGLAKPYEEPSDGQMPDGAVIIAAI 130 140 150 160 170 180 190 200 210 220 230 240 m099.pep TSCTNTSNPRNWAAALLARNANRLGLKRKPWVKSSFAPGSKVAEIYLKEAGLLPEMEKL I I I I I I I I I I I I I I I I I I I I I I I I I I I M I I I I I I I I I I I I I I I I I I I I I I l l l l l l l l a099 TSCTNTSNPRNWAAALLARNANRLGLQRKPWVKSSFAPGSKVAEIYLKEADLLPEMEKL 190 200 210 220 230 240 250 260 270 280 290 300 m099.pep GFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLSGNRNFDGRIHPYAKQAFLASP I I I II I II I II I I II II II I I II II II II II I II II II II II I II I I II I II II I I I I II a099 GFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLSGNRNFDGRIHPYAKQAFLASP 250 260 270 280 290 300 310 320 330 340 350 360 m099. pep PLVVAYALAGSIRFDIENDVLGVADGKEIRLKDIWPADEEIDAWAEYVKPQQFRDVYVP II II II I II II I II I II II I II I II II I II I II II I : II II II : I II I II I I II I II I : I a099 PLWAYALAGSIRFDIENDVLGVADGKEIRLKDIWPTDEEIDAIVAEYVKPQQFRDVYIP 310 320 330 340 350 360 370 380 390 400 410 420 mO 99. pep MFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALAGERTLRGMRPLAILPDNITTDHLSP I II II II II II I II I I I II II I I II II II II II II II II I II II II II II I I II II II I a099 MFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALAGERTLSGMRPLAILPDNITTDHLSP 370 380 390 400 410 420 430 440 450 460 470 480 m099. pep SNAILAVSAAGEYLAKMGLPEEDFNSYATHRGDHLTAQRATFANPKLFNEMVKNEDGSVR llllll I II II I II II I II II II II I I II II II I I II II II I I I I II II I I : I II I I II a099 SNAILASSAAGEYLAKMGLPEEDFNSYATHRGDHLTAQRATFANPKLFNEMVRNEDGSVR 430 440 450 460 470 480 490 500 510 520 530 540 mO 99. pep QGSFARVEPEGETMRMWEAIETYMNRKQPLIIIAGADYGQGSSRDWAAKGVRLAGVEAIV I II : II II II I M I I II II I II I II II I I II II II II II I II II II II I II I II II II II a099 QGSLARVEPEGQTMRMWEAIETYMNRKQPLIIIAGADYGQGSSRDWAAKGVRLAGVEAIV 490 500 510 520 530 540 550 560 570 580 590 600 m099.pep AEGFERIHRTNLIGMGVLPLQFKPDTNRHTLQLDGTETYDWGERTPRCDLTLVIHRKNG II I II II II II I II II II I II I II I II I II II II II II II I II II II I I II II II II II a099 AEGFERIHRTNLIGMGVLPLQFKPGTNRHTLQLDGTETYDWGERTPRCDLTLVIHRKNG 550 560 570 580 590 600 610 620 630 640 mO 99. pep ETVEVPVTCCLDTAEEVLVYEAGGVLQRFAQDFLEGNAAX I I I II I : I I II I II II I II II I I II I II II II I I II II I a099 ETVEVPITCRLDTAEEVLVYEAGGVLQRFAQDFLEGNAAX 610 620 630 640 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 357>: gl02. seq 1 AtgtCCGCCA AAactccgtc gctcttcggc ggcgcgatga Ttatcgccgg 51 gaaggttatc ggcgcAGgta tgttccccaa ccccaccgcc aacttggggg 101 acgggttaat aggctcgctg attgtgctgc tgtacacctg gtttccattc 151 tcctccggcg ccctcatgat tttggaagtc aacacccata acCCccgagg 201 ggcaAGtttt gacaccATGg tcAAagacct gctcgGaCGc ggctggaaca 251 tcatcaacgg catcgccgtc gctttggTCc tatacggctc gacctacgcg 301 tacattttag tcggcggtga cctGACCGCC AAAGGCAtcg GCAgCGCAGT 351 AGGCGGCAAA ATTTCgctca CCGTCGGACA actcgtcttc tTCGGCATCC 401 TCGCCTTTTG CGTATGGGCA TCCGCACGCT TGGTCGACCG CTTTACCGGC 451 GTCCTCATCG GCGGCATGGT ATTAACCTTT ATTTGGGCAA CCGGCGGCCT 501 GGTTGCCGAT GCCAAACCGT CCGTCCTCTT CGACACCCAA GCCCCCGTCG 551 GCACCGGCTA CTGGATTTAC GCCGCCACCG CCCTGCCCGT CTGCCTCGCT 601 TCCTTCGGCT TCCACGGCAA CGTTTCCAGC CTGCTCAAAT ACTTTAAAGG 651 CGACGcgcCc aaagtGgCGA aATCcatctg gGcaggtaca ttggTTGCCt 701 tggtaattta cgtccTCTgg caaaccgcca tCcaaagcaa ccTGCcgcgc 751 aacgagttcg cCCCcgtgat tgccgccgag aggcaactCT CCGTCCTgaa 801 tgaaacccTG tccaaattcg cccaaaccgg cgatatggat aAaatattgt 851 ccctatttcc ctacatggca atcgccacct cctttttagg cgTAACctta 901 ggcctgtttg acaacatcgc cgacatcttc aaatggaacg acagtatgtc 951 cgggcggggc accaaaaccg tcgcgctgaa cttcctgccg CCCCtgattt 1001 cctggctgct cctccccacc ggcttcttta ccgccattgg tgcgtccggc 1051 ctggcggcaa ccgtctggga ccaagGcatc atccccgcca tgctgctcta 1101 cgtttccccc caaaaaattG gcGcaggcaa gacttataAa gtttaCGGCG 1151 gcttgtggct gatgttagtc ttccttttcg gcatcgccaa catcgccgca 1201 CAGGTATTGA GccaAatgGa ACtcgtCccc GTATTTAAAG GATAA This corresponds to the amino acid sequence <SEQ ID 358; ORF 102.ng>: gl02.pep 1 MSAKTPSLFG GAMIIAGKVI GAGMFPNPTA NLGDGLIGSL IVLLYTWFPF 51 SSGALMILEV NTHNPRGASF DTMVKDLLGR GWNIINGIAV ALVLYGSTYA 101 YILVGGDLTA KGIGSAVGGK ISLTVGQLVF FGILAFCVWA SARLVDRFTG 151 VLIGGMVLTF IWATGGLVAD AKPSVLFDTQ APVGTGY IY AATALPVCLA 201 SFGFHGNVSS LLKYFKGDAP KVAKΞI AGT LVALVIYVLW QTAIQSNLPR 251 NEFAPVIAAE RQLSVLNETL SKFAQTGDMD KILSLFPYMA IATSFLGVTL 301 GLFDNIADIF K NDSMΞGRG TKTVALNFLP PLIS LLLPT GFFTAIGASG 351 LAATV DQGI IPAMLLYVSP QKIGAGKTYK VYGGL LMLV FLFGIANIAA 401 QVLSQMELVP VFKG* The following partial DNA sequence was identified in N meningitidis <SEQ ID 359>: ml02. seq 1 ATGCCCAACA AAACCCCTTC ACTGTTCGGC GGCGCGATGA TTATCGCCGG 51 CACGGTCATC GGCGCAGGCA TGCTCGCCAA CCCGACCGCC ACATCCGGCG 101 TATGGTTTAC CGGCTCGCTG GCCGTGTTGC TGTACACCTG GTTTTCTATG 151 CTTTCCAGCG GCCTGATGAT TTTGGAAGTC AACACCCATT ATCCGCACGG 201 CGCAAGTTTC GACACGATGG TCAAAGACCT GCTCGGACGC GGCTGGAACA 251 TCATCAACGG CATCGCCGTC GCCTTCGTTT TATACCTGCT TACTTACGCT 301 TATATCTTCG TCGGCGGCGA CCTGACCGCC AAAGGCTTAG GCAGCGCGGC 351 AGGCGGCGAC GTTTCACTCA CCGTCGGACA ACTCGTCTTC TTCGGCATCC 401 TCGCCTTTTG CGTATGGGCA TCCGCACGCT TGGTCGACCG CTTCACCGGC 451 GTCCTTATCG GCGGCATGGT ATTGACCTTT ATTTGGGCGG CCGGCGGGCT 501 GATTGCCGAT GCCAAGCCGT CCGTCCTCTT CGATACCCAA GCCCCCGCCG 551 GCACAAACTA CTGGATTTAC GCCGCCACCG CCCTGCCCGT CTGCCTCGCT 601 TCCTTCGGCT TCCACGGCAA CGTCTCCAGC CTGCTCAAAT ACTTTAAAGG 651 CGACGCGCCC AAAGTGGCTA AATCCATCTG GACGGGCACA CTGATTGCGC 701 TGGTAATTTA CGTCCTCTGG CAAACCGCCA TCCAAGGCAA CCTGCCGCGC 751 AACGAGTTCG CCCCCGTCAT CGCCGCCGAA GGGCAAGTCT CCGTCCTCAT 801 CGAAACCCTG TCCAAATTCG CCCAAACCGG CAATATGGAC AAAATATTGT 851 CCCTGTTTTC CTATATGGCG ATCGCCACCT CGTTTTTAGG CGTAACGCTC 901 GGACTCTTCG ACTACATCGC CGACATCTTC AAATGGAACG ACAGCATCTC 951 CGGCCGCACC AAAACCGCCG CGCTGACCTT CCTGCCGCCC CTGATTTCCT 1001 GCCTGCTCTT CCCCACCGGC TTCGTTACCG CCATCGGCTA CGTCGGCCTG 1051 GCGGCAACCG TCTGGACAGG CATCATCCCC GCCATGCTGC TCTACCGTTC 1101 GCGCAAAAAA TTCGGCGCAG GCAAAACCTA TAAAGTTTAC GGCGGCTTGT 1151 GGCTGATGGT TTGGGTCTTC CTTTTCGGCA TCGTCAACAT CGCCGCACAG 1201 GTATTGAGCC AAATGGAACT CGTCCCCGTA TTTAAAGGAT AA This corresponds to the amino acid sequence <SEQ ID 360; ORF 102>: ml02.pep. 1 MPΝKTPSLFG GAMIIAGTVI GAGMLAΝPTA TΞGVWFTGSL AVLLYTWFΞM 51 LSΞGLMILEV ΝTHYPHGASF DTMVKDLLGR GWNIINGIAV AFVLYLLTYA 101 YIFVGGDLTA KGLGSAAGGD VSLTVGQLVF FGILAFCVWA SARLVDRFTG 151 VLIGGMVLTF I AAGGLIAD AKPSVLFDTQ APAGTNYWIY AATALPVCLA 201 SFGFHGNVSS LLKYFKGDAP KVAKSIWTGT LIALVIYVLW QTAIQGNLPR 251 NEFAPVIAAE GQVSVLIETL SKFAQTGNMD KILSLFSYMA IATSFLGVTL 301 GLFDYIADIF KWNDSISGRT KTAALTFLPP LIΞCLLFPTG FVTAIGYVGL 351 AATVWTGIIP AMLLYRSRKK FGAGKTYKVY GGLWLMVWVF LFGIVNIAAQ 401 VLSQMELVPV FKG* ml02/gl02 86.0% identity in 415 aa overlap 10 20 30 40 50 60 ml02.pep MPNKTPΞLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLLYTWFSMLSΞGLMILEV I I I I I I I I I I I I I I I llllll: Mil: I : III lllllll : I ::| I I I I I gl02 MSAKTPSLFGGAMIIAGKVIGAGMFPNPTANLGDGLIGSLIVLLYTWFPFSSGALMILEV 10 20 30 40 50 60 70 80 90 100 110 120 ml02. ep NTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYIFVGGDLTAKGLGSAAGGD III I : I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I : I I I : I I gl02 NTHNPRGASFDTMVKDLLGRGWNIINGI VA VLYGSTYAYILVGGDLTAKGIGSAVGGK 70 80 90 100 110 120 130 140 150 160 170 180 Itιl02.pep VSLTVGQLVFFGILAFCVWASARLVDRFTGVLIGGMVLTFIWAAGGLIADAKPSVLFDTQ : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I :| I I : I I I I I I I I I I I I gl02 ISLTVG Q LVFFGILAFCVWASARLVDRFTGVLIGGMVLTFIWATGGLVADAKPSVLFDTQ 130 140 150 160 170 180 190 200 210 220 230 240 ml02. pep APAGTNYWIYAATALPVCLASFGFHGNVSSLLKYFKGDAPKVAKSIWTGTLIALVIYVLW I I : I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I : I I I I I I I I gl02 APVGTGYWIYAATALPVCLASFGFHGNVSSLLKYFKGDAPKVAKSIWAGTLVALVIYVLW 190 200 210 220 230 240 250 260 270 280 290 300 ml02.pep QTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQTGNMDKILSLFSYMAIATSFLGVTL I I I I I : I I I I I I I I I I I I I I hill I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I gl02 QTAIQSNLPRNEFAPVIAAERQLSVLNETLSKFAQTGDMDKILSLFPYMAIATSFLGVTL 250 260 270 280 290 300 310 320 330 340 350 ml02.pep GLFDYIADIFKWNDSISGR-TKTAALTFLPPLISCLLFPTGFVTAIGYVGLAATVWT-GI I I I I I I I I I I I I I I : I I I I I I : I I : I I I I I I I I I : I I I I I I I I lllllll II gl02 GLFDNIADIFKWNDSMSGRGTKTVALNFLPPLISWLLLPTGFFTAIGASGLAATVWDQGI 310 320 330 340 350 360 360 370 380 390 400 410 ml02.pep IPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLFGIVNIAAQVLSQMELVPVFKGX lllllll I : I : I I I I I I I I I I I I I I I : I I I I I I : I I I I I I I I I I I I I I I I I I I gl02 IPAMLLYVSPQKIGAGKTYKVYGGLWLML-VFLFGIANIAAQVLSQMELVPVFKGX 370 380 390 400 410 The following partial DNA sequence was identified in N meningitidis <SEQ ID 361>: al02.seq 1 ATGCCCACCA AAACCCCTTC ACTGTTCGGC GGCGCGATGA TTATCGCCGG 51 CACGΝTCATC GGCGCAGGTA TGCTCGCCAA CCCGACCGCC ACATCCGGCG 101 TATGGTTTAC CGGCTCGCTG GCCGTGTTGC TGTACACCTG GTTTTCCATG 151 CTCTCCAGCG GCCTGATGAT TTTGGAAGTC AACACCCACT ACCCCCACGG 201 CGCGAΝCTTC GACACCATGG TTAAAGACCT GCTCGGACGG AGCTGGAACA 251 TCATCAACGG CATCGCCGTC GCCTTCGTTT TATACCTGCT TACTTACGCT 301 TATATCTTCG TCGGCGGCGA CCTGACCGCC AAAGGCTTAG GCAGCGCGGC 351 AGGCGGCAAT GTTTCACTCA CCGTCGGACA ACTCGTCTTC TTCGGCATTC 401 TCGCCTTTTG CGTATGGGCA TCCGCACGCT TGGTCGACCG ATTCACCAGC 451 GTCCTCATCG GCGGCATGGT ATTAACCTTT ATTTGGGCAA CCGGCGGCCT 501 GATTGCCGAT GCCAAACTGC CCGTCCTCTT CGACACCCAA GCCCCTACCG 551 GCACCAACTA CTGGATTTAT GTCGCCACCG CCCTGCCCGT CTGCCTTGCG 601 TCATTCGGTT TCCACGGCAA CGTCTCCAGC CTGCTCAAAT ACTTTAAAGG 651 CGACGCGCCC AAAGTGGCTA AATCCATCTG GACGGGCACA CTGATTGCGC 701 TGGTAATTTA CGTCCTCTGG CAAACCGCCA TCCAAΝGCAA CCTGCCGCGC 751 AACGAGTTCG CCCCCGTGAT TGCCGCCGAA GGGCAAGTCT CCGTCΝTGAT 801 TGAAACCCTG TCCAAATTCG CCCAAACCGG CAATATGGAC AAAATATTGT 851 CCCTGTTTTC CTATATGGCG ATCGCCACCT CGTTTTTAGG CGTAACGCTC 901 GGACTCTTCG ACTACATCGC CGACATCTTC AAATGGAACG ACAGCGTGTC 951 CGGCCGCACC AAAACCGCCG CGCTGACCTT CCTGCCGCCT ΝTAATTTCCT 1001 GCCTGCTCTT CCCCACCGGC TTTGTTACCG CCATCGGΝTA CGTCGGCCTG 1051 GCGGCAACCG TCTGGACAGG CATCATCCCC GCCATGCTGC TΝTACCGTTC 1101 GCGCAAAAAA TTCGGCGCAG GCAAAACCTA TAAAGTTTAC GGCGGCTTGT 1151 GGCTGATGGT TTGGGTCTTC CTTTTCGGCA TCΝTCAACAT CGCCGCACAΝ 1201 GTATTGAGCC AAATGGAACT CGTCCCCGTA TTTAAAGGAT AA 1202 This corresponds to the amino acid sequence <SEQ ID 362; ORF 102. a>: al02.pep 1 MPTKTPSLFG GAMIIAGTXI GAGMLAΝPTA TSGVWFTGSL AVLLYTWFSM 51 LSSGLMILEV ΝTHYPHGAXF DTMVKDLLGR SWΝIIΝGIAV AFVLYLLTYA 101 YIFVGGDLTA KGLGSAAGGΝ VSLTVGQLVF FGILAFCVWA SARLVDRFTS 151 VLIGGMVLTF IWATGGLIAD AKLPVLFDTQ APTGTΝYWIY VATALPVCLA 201 SFGFHGΝVSS LLKYFKGDAP KVAKSIWTGT LIALVIYVLW QTAIQXΝLPR 251 NEFAPVIAAE GQVSVXIETL SKFAQTGNMD KILSLFSYMA IATSFLGVTL 301 GLFDYIADIF KWNDSVSGRT KTAALTFLPP XISCLLFPTG FVTAIGYVGL 351 AATVWTGIIP AMLLYRSRKK FGAGKTYKVY GGLWLMVWVF LFGIXNIAAX 401 VLSQMELVPV FKG* ml02 / al02 95.9% identity in 413 aa overlap 10 20 30 40 50 60 ml02. pep MPNKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLLYTWFSMLSSGLMILEV II : I II II II I II II I I I I I II II II II II II I II I II I II II II I I II II II II II I I al02 MPTKTPSLFGGAMIIAGTXIGAGMLANPTATSGVWFTGSLAVLLYTWFSMLSSGLMILEV 10 20 30 40 50 60 70 80 90 100 110 120 ml02. pep NTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYIFVGGDLTAKGLGSAAGGD llllllll II I I II II I II : II I II I II II II II I I II I II II II I II II II I II II I : al02 NTHYPHGAXFDTMVKDLLGRSWNIINGIAVAFVLYLLTYAYIFVGGDLTAKGLGSAAGGN 70 80 90 100 110 120 130 140 150 160 170 180 ml02. pep VSLTVGQLVFFGILAFCVWASARLVDRFTGVLIGGMVLTFIWAAGGLIADAKPSVLFDTQ II II I I II II II I II II II II I I I I I II I : II I II II I II II I : I II I I II I llllll al02 VSLTVGQLVFFGILAFCVWASARLVDRFTSVLIGGMVLTFIWATGGLIADAKLPVLFDTQ 130 140 150 160 170 180 190 200 210 220 230 240 ml02. pep APAGTNYWIYAATALPVCLASFGFHGNVSSLLKYFKGDAPKVAKSIWTGTLIALVIYVLW I I M I I II II : II I II II I II II II I I I II II II II II I II II II II I I II I II II II II al02 APTGTNYWIYVATALPVCLASFGFHGNVSSLLKYFKGDAPKVAKSIWTGTLIALVIYVLW 190 200 210 220 230 240 250 260 270 280 290 300 ml02. pep QTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQTGNMDKILSLFSYMAIATSFLGVTL I II II II I I II II II I II II II II II II II II II II I I II I II II I I II I II II II II al02 QTAIQXNLPRNEFAPVIAAEGQVSVXIETLSKFAQTGNMDKILSLFSYMAIATSFLGVTL 250 260 270 280 290 300 310 320 330 340 350 360 ml02.pep GLFDYIADIFKWNDSISGRTKTAALTFLPPLISCLLFPTGFVTAIGYVGLAATVWTGIIP II II II I I II I II II : I I II I II I I II II I II I II II I II II I II II I I II II II II II al02 GLFDYIADIFKWNDΞVSGRTKTAALTFLPPXISCLLFPTGFVTAIGYVGLAATVWTGIIP 310 320 330 340 350 360 370 380 390 400 410 ml02. pep AMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLFGIVNIAAQVLSQMELVPVFKGX I II I II I II I II II II I I I I II II II I II II I II II I I II II II I II II II I al02 AMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLFGIXNIAAXVLSQMELVPVFKGX 370 380 390 400 410 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 363>: gl05.seq 1 Atgtccgcag aaaCATACAc acAAAtcggc tGGgtaggct taggGcaaat 51 gGgtctgcct atgGTAACGC GGCTCTTGGA CGGCGGCATC GAAGTCGGCG 101 TATACAACCG CTCGCCCGAC AAAACTGCCC CCATCTCcgc CAAAGGAGCA 151 AAAGTTTACG GCagcACCGC CGAACTCGTC CGCGCCTGCC CCGTCATTTT 201 CCTGATGGTT TCCGACTATG CCGCCGTGTG CGACATCCTG AACGGAGTCC 251 GCGACGGATT GGCCGGCAAA ATCATCGTCA ACATGAGCAC CATCTCCCCG 301 ACCGAAAACC TCGCCGTCAA AGCACTTGTC GAAGCCGCAG GCGGACAGTT 351 TGCCGAAGCA CCCGTTTCCG GATCGGTCGG ACCCGCCACC AACGGCACAC 401 TGCTGATTCT GTTCGGCGGC AGCGAAGCCG TTTTAAACCC GCTGCAAAAA 451 ATATTTTCCC TTGTCGGCAA AAAAACCTTC CATTTCGGCG ATGTCGGCAA 501 AGGCTCGGGC GCGAAACTCG TCTTGAACTC GCTCTTAGGC ATTTTCGGCG 551 AAGCGTACAG CGAAGCGATG CTGATGGCGC GGCAGTTCGG CATCGATACC 601 GACACCATCG TCGAAGCCAT CGGCGGCTCG GCAATGGACT CGCCTATGTT 651 TCAAACAAAA AAATCACTAT GGGCAAACCG TGAGTTCCCC CCTGCCTTTG 701 CACTCAAACA CGCTTCCAAA GAcctTAACC TCGccgtcAA AGAGCTTGAA 751 CAGGCAGGCA ACACCCTGCC CGCCGTCGAA ACCGTTGCTG CCAGCTACCG 801 CAAAGCAGTT GAAGCCGGCT ACGGCGAACA GGACGTTTCC GGCGTTTACC 851 TGAAATTGGC AGAACACTGA This corresponds to the amino acid sequence <SEQ ID 364; ORF 105.ng>: gl05.pep 1 MSAETYTQIG WVGLGQMGLP MVTRLLDGGI EVGVYNRSPD KTAPISAKGA 51 KVYGSTAELV RACPVIFLMV SDYAAVCDIL NGVRDGLAGK IIVNMSTISP 101 TENLAVKALV EAAGGQFAEA PVSGSVGPAT NGTLLILFGG SEAVLNPLQK 151 IFSLVGKKTF HFGDVGKGSG AKLVLNSLLG IFGEAYSEAM LMARQFGIDT 201 DTIVEAIGGS AMDSPMFQTK KSLWANREFP PAFALKHASK DLNLAVKELE 251 QAGNTLPAVE TVAASYRKAV EAGYGEQDVS GVYLKLAEH The following partial DNA sequence was identified in N meningitidis <SEQ ID 365>: ml05. seq 1 ATGTCCGCAA ACGAATACGC ACAAATCGGC TGGaTAGGCT TAGGGCAAAT 51 GGGTCTGCCT ATGGTAACGC GGCTCTTGGA CGGCGGCATC GAAGTCGGCG 101 TATACAACCG CTCGCCCGAC AAAACTGCCC CCATCTCCGC CAAAGGCGCA 151 AAAGTTTACG GCAACACCGC CGAACTCGTC CGCGACTATC CCGTCATTTT 201 CCTGATGGTT TCCGACTATG CCGCCGTGTG CGACATCCTG AACGGAGTCC 251 GCGACGGATT GGCCGGCAAm ATCATCGTCA ACATGAGCAC CATCTCCCCG 301 ACCGAAAaGC TCGCCGTCAA AGCACTTGTC GAAGCGCAGm GaCAGTTTGC 351 CGAAGCACCC GTTTCCGGAT CGGTCGGGCC CGCCACCAAC GGCACGCTGC 401 TGATTCTGTT CGGCGGCAGC GAAcCGtTTT AAACCCGCTG CAAAAAATAT 451 TTTCCCTCGT CGGCAAAAAA ACCTTCCATT TCGGCGATGT CGGCAAAGGT 501 TCGGGCGCGA AACTCGTCTT GAACTCGCTC TTGGGCATTT TCGGCGAaCG 551 TAcAGCGAAs GmTgCTGATG GCGCGGCAGT TCGGCATCGA TACCGACACC 601 ATCGTCGAAG CCATCGGsGA CTCGGCAATG GACTCGCCCA TGTTCCAAAC 651 CAAAAAATCC CTGTGGGCAA ACCGCGAATT CCCGmCCGmC TTCGCCCTCA 701 AACACGCCTC CAAAGACCTC AACCTCGCCG TCAAAGAGCT TGAACAGGCA 751 GGCAACACCC TGCCCGCCGT CGAAACCGTT GCTGCCAGCT ACCGCAAAGC 801 AGTCGAAGCC GGCTACGGGA CACAGGACGT TTCCGGCGTT TACCTGAAAC 851 TGGCAGAACA CTGA This corresponds to the amino acid sequence <SEQ ID 366; ORF 105>: ml05.pep 1 MSAΝEYAQIG WIGLGQMGLP MVTRLLDGGI EVGVYNRSPD KTAPISAKGA 51 KVYGNTAELV RDYPVIFLMV SDYAAVCDIL NGVRDGLAGX IIVNMSTISP 101 TEKLAVKALV EAQRQFAEAP VSGSVGPATN GTLLILFGGS EPFXTRCKKY 151 FPSSAKKPSI SAMSAKVRAR NSSXTRSWAF SANVQRXXLM ARQFGIDTDT 201 IVEAIGDSAM DSPMFQTKKS LWANREFPXX FALKHASKDL NLAVKELEQA 251 GNTLPAVETV AASYRKAVEA GYGTQDVSGV YLKLAEH Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 105 shows 79.9%> identity over a 289 aa overlap with a predicted ORF (ORF 105.ng) from N gonorrhoeae: ml05/gl05 10 20 30 40 50 60 gl05. ep MSAETYTQIGWVGLGQMGLPMVTRLLDGGIEVGVYΝRSPDKTAPISAKGAKVYGSTAELV M M I M M I M M M M M M M M M M M M M I M I M M M M I M M M M M ml05 MSAΝEYAQIGWIGLGQMGLPMVTRLLDGGIEVGVYΝRSPDKTAPISAKGAKVYGΝTAELV 10 20 30 40 50 60 70 80 90 100 110 120 gl05.pep RACPVIFLMVSDYAAVCDILΝGVRDGLAGKIIVΝMSTISPTEΝLAVKALVEAAGGQFAEA I M M M M M M M M M M M M M I I I I I I 1 1 I I 1 1 M 1 1 I I I I I I M i l l ml05 RDYPVIFLMVSDYAAVCDILΝGVRDGLAGXI IVΝMSTISPTEKLAVKALVEAQR-QFAEA 70 80 90 100 110 130 140 150 160 170 180 gl05. pep PVSGSVGPATΝGTLLILFGGSEAVLΝPLQKIFSLVGKKTFHFGDVGKGSGAKLVLΝSLLG : M I Ml :: :: M ml05 PVSGSVGPATΝGTLLILFGGSEPFXTRCKKYFPSSAKKP-SISAMSAKVRARΝSSXTRSW 120 130 140 150 160 170 190 200 210 220 230 240 gl05.pep IFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQTKKSLWANREFPPAFALKHASK M = : M M M M M M I M M M M ml05 AFSANVQRXXLMARQFGIDTDTIVEAIGDSAMDSPMFQTKKSLWANREFPXXFALKHASK 180 190 200 210 220 230 250 260 270 280 289 gl05.pep DLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGEQDVSGVYLKLAEH 11 ! I i 111 ! 111111 M 11 I ! 11 i 1111111 M 11 I i I ! M ! I ! 111 I ml05 DLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGTQDVSGVYLKLAEH 240 250 260 270 280 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 367>: al05. seq 1 ATGTCCGCAA ACGAATACAC ACAAATCGGC TGGATAGGCT TAGGGCAAAT 51 GGGTCTGCCT ATGGTAACGC GGCTCTTGGA CGGCGGCATC GAAGTCGGCG 101 TATACAACCG CTCGCCCGAC AAAACTGCCC CCATCTCCGC CAAAGGCGCA 151 AAAGTTTACG GCAACACCGC CGAACTCGTC CGCGACTATC CCGTCATTTT 201 CCTGATGGTT TCCGACTATG CCGCCGTGTG CGACATCCTG AACGGAGTCC 251 GCGACGGATT GGCCGGCAAA ATCATCGTCA ACATGAGCAC CATCTCCCCG 301 ACCGAAAACC TCGCCGTCAA AGCACTTGTC GAAGCCGCAG GCGGACAGTT 351 TGCCGAAGCA CCCGTTTCCG GATCGGTCGG GCCCGCCACC AACGGCACGC 401 TGCTGATTCT GTTCGGCGGC AGCGAAGCCG TTTTAAACCC GCTGCAAAAA 451 ATATTTTCCC TCGTCGGCAA AAAAACCTTC CATTTCGGCG ATGTCGGCAA 501 AGGTTCGGGC GCGAAACTCG TCTTGAACTC GCTCTTGGGC ATTTTCGGCG 551 AAGCGTACAG CGAAGCGATG CTGATGGCGC GGCAGTTCGG CATCGATACC 601 GACACCATCG TCGAAGCCAT CGGCGGCTCG GCAATGGACT CGCCCATGTT 651 CCAAACCAAA AAATCCCTGT GGGCAAACCG CGAATTCCCA CCCGCCTTCG 701 CCCTCAAACA CGCCTCCAAA GACCTCAACC TCGCCGTCAA AGAGCTTGAA 751 CAGGCAGGCA ACACCCTGCC CGCCGTCGAA ACCGTTGCTG CCAGCTACCG 801 CAAAGCAGTC GAAGCCGGCT ACGGCGAACA GGACGTTTCC GGCGTTTACC 851 TGAAATTGGC AGAACACTGA This corresponds to the amino acid sequence <SEQ ID 368; ORF 105.a>: al05.pep 1 MSAΝEYTQIG WIGLGQMGLP MVTRLLDGGI EVGVYNRSPD KTAPISAKGA 51 KVYGNTAELV RDYPVIFLMV SDYAAVCDIL NGVRDGLAGK IIVNMSTISP 101 TENLAVKALV EAAGGQFAEA PVSGSVGPAT NGTLLILFGG SEAVLNPLQK 151 IFSLVGKKTF HFGDVGKGSG AKLVLNSLLG IFGEAYSEAM LMARQFGIDT 201 DTIVEAIGGS AMDSPMFQTK KSLWANREFP PAFALKHASK DLNLAVKELE 251 QAGNTLPAVE TVAASYRKAV EAGYGEQDVS GVYLKLAEH* ml05/al05 96.5% identity in 289 aa overlap 10 20 30 40 50 60 ml05. pep MSANEYAQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAPISAKGAKVYGNTAELV II II II : I II I II II II I II I II I II II I II I II I II I I I I II II I II II I I I I II II II al05 MSANEYTQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAPISAKGAKVYGNTAELV 10 20 30 40 50 60 70 80 90 100 110 119 ml05. pep RDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTENLAVKALVEAAG-QFAEA II I I I II II II I II II I II I II I II II I II II II II I II II II I II I I I II I II II II I al05 RDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTENLAVKALVEAAGGQFAEA 70 80 90 100 110 120 120 130 140 150 160 170 179 ml05. pep PVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTFHFGDVGKGSGAKLVLNSLLG II I I I II II I II II II I II I II I II II II I II II II II I I I II II II II II II I II I I II al05 PVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTFHFGDVGKGSGAKLVLNSLLG 130 140 150 160 170 180 180 190 200 210 220 230 ml05. pep IFGDV-QRXMLMARQFGIDTDTIVEAIGDSAMDSPMFQTKKSLWANREFPXAFALKHASK III:: : : II I II II II II II II I II I II II II II I II II II I II I II lllllllll al05 IFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQTKKSLWANREFPPAFALKHASK 190 200 210 220 230 240 240 250 260 270 280 ml 05. pep DLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGEQDVSGVYLKLAEHX II II II II I II II I I I I II II I II II II II II I II I II II II II I I I I I I al05 DLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGEQDVSGVYLKLAEHX 250 260 270 280 290 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 369>: gl05-l.seq 1 ATGTCCGCAG AAACATACAC ACAAATCGGC TGGGTAGGCT TAGGGCAAAT 51 GGGTCTGCCT ATGGTAACGC GGCTCTTGGA CGGCGGCATC GAAGTCGGCG 101 TATACAACCG CTCGCCCGAC AAAACTGCCC CCATCTCCGC CAAAGGAGCA 151 AAAGTTTACG GCAGCACCGC CGAACTCGTC CGCGCCTGCC CCGTCATTTT 201 CCTGATGGTT TCCGACTATG CCGCCGTGTG CGACATCCTG AACGGAGTCC 251 GCGACGGATT GGCCGGCAAA ATCATCGTCA ACATGAGCAC CATCTCCCCG 301 ACCGAAAACC TCGCCGTCAA AGCACTTGTC GAAGCCGCAG GCGGACAGTT 351 TGCCGAAGCA CCCGTTTCCG GATCGGTCGG ACCCGCCACC AACGGCACAC 401 TGCTGATTCT GTTCGGCGGC AGCGAAGCCG TTTTAAACCC GCTGCAAAAA 451 ATATTTTCCC TTGTCGGCAA AAAAACCTTC CATTTCGGCG ATGTCGGCAA 501 AGGCTCGGGC GCGAAACTCG TCTTGAACTC GCTCTTAGGC ATTTTCGGCG 551 AAGCGTACAG CGAAGCGATG CTGATGGCGC GGCAGTTCGG CATCGATACC 601 GACACCATCG TCGAAGCCAT CGGCGGCTCG GCAATGGACT CGCCTATGTT 651 TCAAACAAAA AAATCACTAT GGGCAAACCG TGAGTTCCCC CCTGCCTTTG 701 CACTCAAACA CGCTTCCAAA GACCTTAACC TCGCCGTCAA AGAGCTTGAA 751 CAGGCAGGCA ACACCCTGCC CGCCGTCGAA ACCGTTGCTG CCAGCTACCG 801 CAAAGCAGTT GAAGCCGGCT ACGGCGAACA GGACGTTTCC GGCGTTTACC 851 TGAAATTGGC AGAACACTGA This corresponds to the amino acid sequence <SEQ ID 370; ORF 105-1. ng>: gl05-l.pep 1 MSAETYTQIG WVGLGQMGLP MVTRLLDGGI EVGVYNRSPD KTAPISAKGA 51 KVYGSTAELV RACPVIFLMV SDYAAVCDIL NGVRDGLAGK IIVNMSTISP 101 TENLAVKALV EAAGGQFAEA PVSGSVGPAT NGTLLILFGG ΞEAVLNPLQK 151 IFSLVGKKTF HFGDVGKGSG AKLVLNΞLLG IFGEAYSEAM LMARQFGIDT 201 DTIVEAIGGS AMDΞPMFQTK KSLWANREFP PAFALKHASK DLNLAVKELE 251 QAGNTLPAVE TVAASYRKAV EAGYGEQDVS GVYLKLAEH* The following partial DNA sequence was identified in N meningitidis <SEQ ID 371>: ml05-l. seq 1 ATGTCCGCAA ACGAATACGC ACAAATCGGC TGGATAGGCT TAGGGCAAAT 51 GGGTCTGCCT ATGGTAACGC GGCTCTTGGA CGGCGGCATC GAAGTCGGCG 101 TATACAACCG CTCGCCCGAC AAAACTGCCC CCATCTCCGC CAAAGGCGCA 151 AAAGTTTACG GCAACACCGC CGAACTCGTC CGCGACTATC CCGTCATTTT 201 CCTGATGGTT TCCGACTATG CCGCCGTGTG CGACATCCTG AACGGAGTCC 251 GCGACGGATT GGCCGGCAAA ATCATCGTCA ACATGAGCAC CATCTCCCCG 301 ACCGAAAACC TCGCCGTCAA AGCACTTGTC GAAGCCGCAG GCGGACAGTT 351 TGCCGAAGCA CCCGTTTCCG GATCGGTCGG GCCCGCCACC AACGGCACGC 401 TGCTGATTCT GTTCGGCGGC AGCGAAGCcG TTTTAAACCC GCTGCAAAAA 451 ATATTTTCCC TCGTCGGCAA AAAAACCTTC CATTTCGGCG ATGTCGGCAA 501 AGGTTCGGGC GCGAAACTCG TCTTGAACTC GCTCTTGGGC ATTTTCGGCG 551 AAGCGTACAG CGAAnCGATG CTGATGGCGC GGCAGTTCGG CATCGATACC 601 GACACCATCG TCGAAGCCAT CGGsGACTCG GCAATGGACT CGCCCATGTT 651 CCAAACCAAA AAATCCCTGT GGGCAAACCG CGAATTCCCG CCCGCCTTCG 701 CCCTCAAACA CGCCTCCAAA GACCTCAACC TCGCCGTCAA AGAGCTTGAA 751 CAGGCAGGCA ACACCCTGCC CGCCGTCGAA ACCGTTGCTG CCAGCTACCG 801 CAAAGCAGTC GAAGCCGGCT ACGGCGAACA GGACGTTTCC GGCGTTTACC 851 TGAAACTGGC AGAACACTGA This corresponds to the amino acid sequence <SEQ ID 372; ORF 105-1>: l05-l.pep 1 MΞAΝEYAQIG WIGLGQMGLP MVTRLLDGGI EVGVYNRSPD KTAPISAKGA 51 KVYGNTAELV RDYPVIFLMV SDYAAVCDIL NGVRDGLAGK IIVNMSTISP 101 TENLAVKALV EAAGGQFAEA PVSGSVGPAT NGTLLILFGG SEAVLNPLQK 151 IFSLVGKKTF HFGDVGKGSG AKLVLNSLLG IFGEAYSEXM LMARQFGIDT 201 DTIVEAIGDS AMDSPMFQTK KSLWANREFP PAFALKHASK DLNLAVKELE 251 QAGNTLPAVE TVAASYRKAV EAGYGEQDVS GVYLKLAEH* l05-l/gl05-l 96.9% identity in 289 aa overlap 10 20 30 40 50 60 ml05-1. pep MSANEYAQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAPISAKGAKVYGNTAELV III: I : I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I gl05-l MSAETYTQIGWVGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAPISAKGAKVYGSTAELV 10 20 30 40 50 60 70 80 90 100 110 120 ml05-1. pep RDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTENLAVKALVEAAGGQFAEA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl05-1 RACPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTENLAVKALVEAAGGQFAEA 70 80 90 100 110 120 130 140 150 160 170 180 ml05-l.pep PVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTFHFGDVGKGSGAKLVLNSLLG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl05-l PVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTFHFGDVGKGSGAKLVLNSLLG 130 140 150 160 170 180 190 200 210 220 230 240 ml05-l.pep IFGEAYΞEXMLMARQFGIDTDTIVEAIGDSAMDSPMFQTKKSLWANREFPPAFALKHASK llllllll I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl05-l IFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQTKKSLWANREFPPAFALKHASK 190 200 210 220 230 240 250 260 270 280 290 ml05-l .pep DLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGEQDVSGVYLKLAEHX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl05-l DLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGEQDVSGVYLKLAEHX 250 260 270 280 290 The following partial DNA sequence was identified in N meningitidis <SEQ ID 373>: al05-l.seq 1 ATGTCCGCAA ACGAATACAC ACAAATCGGC TGGATAGGCT TAGGGCAAAT 51 GGGTCTGCCT ATGGTAACGC GGCTCTTGGA CGGCGGCATC GAAGTCGGCG 101 TATACAACCG CTCGCCCGAC AAAACTGCCC CCATCTCCGC CAAAGGCGCA 151 AAAGTTTACG GCAACACCGC CGAACTCGTC CGCGACTATC CCGTCATTTT 201 CCTGATGGTT TCCGACTATG CCGCCGTGTG CGACATCCTG AACGGAGTCC 251 GCGACGGATT GGCCGGCAAA ATCATCGTCA ACATGAGCAC CATCTCCCCG 301 ACCGAAAACC TCGCCGTCAA AGCACTTGTC GAAGCCGCAG GCGGACAGTT 351 TGCCGAAGCA CCCGTTTCCG GATCGGTCGG GCCCGCCACC AACGGCACGC 401 TGCTGATTCT GTTCGGCGGC AGCGAAGCCG TTTTAAACCC GCTGCAAAAA 451 ATATTTTCCC TCGTCGGCAA AAAAACCTTC CATTTCGGCG ATGTCGGCAA 501 AGGTTCGGGC GCGAAACTCG TCTTGAACTC GCTCTTGGGC ATTTTCGGCG 551 AAGCGTACAG CGAAGCGATG CTGATGGCGC GGCAGTTCGG CATCGATACC 601 GACACCATCG TCGAAGCCAT CGGCGGCTCG GCAATGGACT CGCCCATGTT 651 CCAAACCAAA AAATCCCTGT GGGCAAACCG CGAATTCCCA CCCGCCTTCG 701 CCCTCAAACA CGCCTCCAAA GACCTCAACC TCGCCGTCAA AGAGCTTGAA 751 CAGGCAGGCA ACACCCTGCC CGCCGTCGAA ACCGTTGCTG CCAGCTACCG 801 CAAAGCAGTC GAAGCCGGCT ACGGCGAACA GGACGTTTCC GGCGTTTACC 851 TGAAATTGGC AGAACACTGA This corresponds to the amino acid sequence <SEQ ID 374; ORF 105-l.a>: al05-l.pep 1 MSAΝEYTQIG WIGLGQMGLP MVTRLLDGGI EVGVYNRSPD KTAPISAKGA 51 KVYGNTAELV RDYPVIFLMV SDYAAVCDIL NGVRDGLAGK IIVNMSTISP 101 TENLAVKALV EAAGGQFAEA PVSGSVGPAT NGTLLILFGG SEAVLNPLQK 151 IFSLVGKKTF HFGDVGKGSG AKLVLNSLLG IFGEAYSEAM LMARQFGIDT 201 DTIVEAIGGS AMDSPMFQTK KSLWANREFP PAFALKHASK DLNLAVKELE 251 QAGNTLPAVE TVAASYRKAV EAGYGEQDVS GVYLKLAEH* al05-l/ml05-l 99.0% identity in 289 aa overlap 10 20 30 40 50 60 al05-l.pep MSANEYTQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAPISAKGAKVYGNTAELV I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml05-1 MSANEYAQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAPISAKGAKVYGNTAELV 10 20 30 40 50 60 70 80 90 100 110 120 al05-1. pep RDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTENLAVKALVEAAGGQFAEA I M I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M I I I I I I I I I I I I I I I I I I ml05-1 RDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTENLAVKALVEAAGGQFAEA 70 80 90 100 110 120 130 140 150 160 170 180 al05-1. pep PVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTFHFGDVGKGSGAKLVLNSLLG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml05-1 PVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTFHFGDVGKGSGAKLVLNSLLG 130 140 150 160 170 180 190 200 210 220 230 240 al05-l pep IFGEAYΞEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQTKKSLWANREFPPAFALKHASK l l l l l l l l II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml05-1 IFGEAYSEXMLMARQFGIDTDTIVEAIGDSAMDSPMFQTKKSLWANREFPPAFALKHASK 190 200 210 220 230 240 250 260 270 280 290 al05-l .pep DLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGEQDVSGVYLKLAEHX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml05-1 DLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGEQDVSGVYLKLAEHX 250 260 270 280 290 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 375>: gl07.seq 1 ATGGTATTAA CCTTTATTTG GGCAACCGGC GGCCTGGTTG CCGATGCCAA 51 ACCGTCCGTC CTCTTCGACA CCCAAGCCCC CGTCGGCACC GGCTACTGGA 101 TTTACGCCGC CACCGCCCTG CCCGTCTGCC TCGCTTCCTT CGGCTTCCAC 151 GGCAACGTTT CCAGCCTGCT CAAATACTTT AAAGGCGACG cgcCcaaagt 201 GgCGAaATCc atctggGcag gtacattggT TGCCttggta atttacgtcc 251 TCTggcaaac cgccatCcaa agcaaccTGC cgcgcaacga gttcgcCCCc 301 gtgattgccg ccgagaggca actCTCCGTC CTgaatgaaa cccTGtccaa 351 attcgcccaa accggcgata tggataAaat attgtcccta tttccctaca 401 tggcaatcgc cacctccttt ttaggcgTAA Ccttaggcct gtttgacaac 451 atcgccggac atcttcaaat ggaacgacag tatgtccggg cggcaccaaa 501 accgtcgcgc tga This corresponds to the amino acid sequence <SEQ ID 376; ORF 107.ng>: gl07.pep 1 MVLTFIWATG GLVADAKPSV LFDTQAPVGT GYWIYAATAL PVCLASFGFH 51 GΝVSSLLKYF KGDAPKVAKS IWAGTLVALV IYVLWQTAIQ SΝLPRΝEFAP 101 VIAAERQLSV LΝETLSKFAQ TGDMDKILSL FPYMAIATSF LGVTLGLFDΝ 151 IAGHLQMERQ YVRAAPKPSR * The following partial DΝA sequence was identified in N meningitidis <SEQ ID 377>: ml07. seq 1 ATGGTATTGA CCTTTATTTG GGCGGCCGGC GGGCTGATTG CCGATGCCAA 51 GCCGTCCGTC CTCTTCGATA CCCAAGCCCC CGCCGGCACA AACTACTGGA 101 TTTACGCCGs CACCGCCCTG CCCGTCTGCC TCGCTTCCTT CGGCTTCCAC 151 GGCAACGTCT CCAGCCTGCT CAAATACTTT AAAGGCGACG CGCCCAAAGT 201 GGCTAAATCC ATCTGGACGG GCACACTGAT TGCGCTGGTA ATTTACGTCC 251 TCTGGCAAAC CGCCATCCAA GGCAACCTGC CGCGCAACGA GTTCGCCCCC 301 GTCATCGCCG CCGAAGGGCA AGTCTCCGTC CTCATCGAAA CCCTGTCCAA 351 ATTCGCCCAA ACCGGCAATA TGGACAAAAT ATTGTCCCTG TTTTCCTATA 401 TGGCGATCGC CACCTCGTTT TTAGGCGTAA CGCTCGGACT CTTCGACTAC 451 ATCGCCCATC TTCAAATGGA ACGACAGCAT CTCCGGgCCG CACCAAAACC 501 GCCGCGCTGA This corresponds to the amino acid sequence <SEQ ID 378; ORF 107>: ml07.pep.. 1 MVLTFIWAAG GLIADAKPSV LFDTQAPAGT ΝYWIYAXTAL PVCLASFGFH 51 GΝVSSLLKYF KGDAPKVAKS IWTGTLIALV IYVLWQTAIQ GΝLPRΝEFAP 101 VIAAEGQVSV LIETLSKFAQ TGNMDKILSL FSYMAIATSF LGVTLGLFDY 151 IAHLQMERQH LRAAPKPPR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 107 shows 89.4% identity over a 170 aa overlap with a predicted ORF (ORF 107.ng) from N gonorrhoeae: ml07/gl07 10 20 30 40 50 60 ml07.pep MVLTFIWAAGGLIADAKPSVLFDTQAPAGTΝYWIYAXTALPVCLASFGFHGΝVSSLLKYF MMMMMMMMMMMIMMMIMMM I II II II I II I II M I II I II II gl07 MVLTFIWATGGLVADAKPSVLFDTQAPVGTGYWIYAATALPVCLASFGFHGΝVSSLLKYF 10 20 30 40 50 60 70 80 90 100 110 120 ml07. pep KGDAPKVAKSIWTGTLIALVIYVLWQTAIQGΝLPRΝEFAPVIAAEGQVSVLIETLSKFAQ MMMMMMMMMMMMMMMMIMMMMMM Mill IIIMIII gl07 KGDAPKVAKSIWAGTLVALVIYVLWQTAIQSΝLPRΝEFAPVIAAERQLSVLΝETLSKFAQ 70 80 90 100 110 120 130 140 150 160 170 ml07.pep TGΝMDKILSLFSYMAIATSFLGVTLGLFDYIA-HLQMERQHLRAAPKPPR MMMIIIM II MMMMMIMM I gl07 TGDMDKILSLFPYMAIATSFLGVTLGLFDΝIAGHLQMERQYVRAAPKPSR 130 140 150 160 170 The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 379>: al07.seq 1 ATGGTATTAA CCTTTATTTG GGCAACCGGC GGCCTGATTG CCGATGCCAA 51 ACTGCCCGTC CTCTTCGACA CCCAAGCCCC TACCGGCACC AACTACTGGA 101 TTTATGTCGC CACCGCCCTG CCCGTCTGCC TTGCGTCATT CGGTTTCCAC 151 GGCAACGTCT CCAGCCTGCT CAAATACTTT AAAGGCGACG CGCCCAAAGT 201 GGCTAAATCC ATCTGGACGG GCACACTGAT TGCGCTGGTA ATTTACGTCC 251 TCTGGCAAAC CGCCATCCAA GGCAACCTGC CGCGCAACGA GTTCGCCCCC 301 GTGATTGCCG CCGAAGGGCA AGTCTCCGTC CTGATTGAAA CCCTGTCCAA 351 ATTCGCCCAA ACCGGCAATA TGGACAAAAT ATTGTCCCTG TTTTCCTATA 401 TGGCGATCGC CACCTCGTTT TTAGGCGTAA CGCTCGGACT CTTCGACTAC 451 ATCGCCGACA TCTTCAAATG GAACGACAGC GTGTCCGGCC GCACCAAAAC 501 CGCCGCGCTG ACCTTCCTGC CGCCTCTAAT TTCCTGCCTG CTCTTCCCCA 551 CCGGCTTTGT TACCGCCATC GGCTACGTCG GCCTGGCGGC AACCGTCTGG 601 ACAGGCATCA TCCCCGCCAT GCTGCTCTAC CGTTCGCGCA AAAAATTCGG 651 CGCAGGCAAA ACCTATAAAG TTTACGGCGG CTTGTGGCTG ATGGTTTGGG 701 TCTTCCTTTT CGGCATCGTC AACATCGCCG CACAGGTATT GAGCCAAATG 751 GAACTCGTCC CCGTATTTAA AGGATAA This corresponds to the amino acid sequence <SEQ ID 380; ORF 107.a>: al07.pep 1 MVLTFIWATG GLIADAKLPV LFDTQAPTGT ΝYWIYVATAL PVCLASFGFH 51 GΝVSSLLKYF KGDAPKVAKS IWTGTLIALV IYVLWQTAIQ GΝLPRΝEFAP 101 VIAAEGQVSV LIETLSKFAQ TGNMDKILSL FSYMAIATSF LGVTLGLFDY 151 IADIFKWNDS VSGRTKTAAL TFLPPLISCL LFPTGFVTAI GYVGLAATVW 201 TGIIPAMLLY RSRKKFGAGK TYKVYGGLWL MVWVFLFGIV NIAAQVLSQM 251 ELVPVFKG* l07/al07 94.8% identity in 154 aa overlap 10 20 30 40 50 60 ml07.pep MVLTFIWAAGGLIADAKPSVLFDTQAPAGTNYWIYAXTALPVCLASFGFHGNVSSLLKYF I I I I I I I i I I I I I I I I I I I I I I 1:1 I I I I I I: I I I I I I I I I I I I I I I I I I I I I I I al07 MVLTFIWATGGLIADAKLPVLFDTQAPTGTNYWIYVATALPVCLASFGFHGNVSSLLKYF 10 20 30 40 50 60 70 80 90 100 110 120 ml07. pep KGDAPKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQ II II II II I II II I II II I II I I II II II I II II I II II II II II II I II II I II I I II I al07 KGDAPKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQ 70 80 90 100 110 120 130 140 150 160 170 ml07.pep TGNMDKILSLFSYMAIATSFLGVTLGLFDYIAHLQMERQHLRAAPKPPRX I I II II II I II I II II II I II I II I II II II I : al07 TGNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSVSGRTKTAALTFLPPLISCL 130 140 150 160 170 180 al07 LFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLFGIV 190 200 210 220 230 240 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 381>: gl08. seq 1 ATGttgccgg gCTTCAACCG GATATTCAaa cggTTTGCTC CAACACTCGG 51 AAcggCGCAT AAAACGCCgc ccTTCGCGTT ATCCCGAACG GGGCGGCTAA 101 TCAGATCCTA TCGCCATAAA AGGCGGGGTT TCAACCGAAA AGGAATTGAG 151 ATGAATAAAA CCTTGTCTAT TTTGCCGGCG GCAATCTTAC TCGGCGGGTG 201 CGCCGCCGGC GGCAACACAT TCGGCAGCTT AGACGGCGGC ACGGGTATGG 251 GTGGCAGCAT CGTCAAAATG ACGGTAGAAA gccAATGCCG TGCGGAATTG 301 GACAGGCGCA GCGAATGGCG TTTGACCGCG CTGGCGATGA GTGCCGAAAA 351 ACAGGCGGAA TGGGAAAACA AGATTTGCGG CTGCGCTACC GAAGAAGCAC 401 CTAACCAGCT GACCGGCAAC GATGTGATGC AGATGCTGAa ccagtccacG 451 CGCaatcagg cacTtgccgc CCtgaccgTC AAAacggtTT CcgcctgcTT 501 CAaacgcctg tACCGCTAa This corresponds to the amino acid sequence <SEQ ID 382; ORF 108.ng>: glOδ.pep 1 MLPGFΝRIFK RFAPTLGTAH KTPPFALSRT GRLIRSYRHK RRGFΝRKGIE 51 MΝKTLSILPA AILLGGCAAG GΝTFGSLDGG TGMGGSIVKM TVESQCRAEL 101 DRRSEWRLTA LAMSAEKQAE WEΝKICGCAT EEAPΝQLTGΝ DVMQMLΝQST 151 RΝQALAALTV KTVSACFKRL YR* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 383>: ml08. seq 1 ATGTTGCCGG GCTTCAACCG GATATTCAAA CGGTTTGTTC CAACACTCGG 51 AACGGCGCAT AAAACGCCGC CCTTCGCGTT ATCCCGAACG GGGCGGCTAA 101 TCAGATTCTA TCGCCATAAA AGGCGGGGTT TCAACCGAAA AGGAATTGAG 151 ATGAATAAAA CCTTGTCTAT TTTGCCGGTG GCAATCTTAC TCGGCGGCTG 201 CGCCGCCGGA GGCGGTAACA CATTCGGCAG CTTAGACGGT GGCACAGGCA 251 TGGGCGGCAG CATCGTCAAA ATGGCGGTTG GGAGCCAATG CCGTGCGGAA 301 TTGGACAAAC GCAGCGAATG GCGTTTGACC GCGCTGGCGA TGAGTGCCGA 351 AAAACAGGCG GAGTGGGAAA ACAAGATTTG CGCTTGCGTC GCCCAAGAAG 401 CACCCGAACG GATGACCGGC AACGATGTGA TGCAGATGCT GGCTCCGTCC 451 ACGCGCAATC AGGCACTTGC CGCCCTGACC GCCAAAACGG TTTCCGCCTG 501 CTTCAAACAC CTGTACCGCT AA This corresponds to the amino acid sequence <SEQ ID 384; ORF 108>: ml08.pep 1 MLPGFΝRIFK RFVPTLGTAH KTPPFALSRT GRLIRFYRHK RRGFΝRKGIE 51 MΝKTLSILPV AILLGGCAAG GGΝTFGSLDG GTGMGGSIVK MAVGSQCRAE 101 LDKRSEWRLT ALAMSAEKQA EWEΝKICACV AQEAPERMTG ΝDVMQMLAPS 151 TRΝQALAALT AKTVSACFKH LYR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 108 shows 89.6% identity over a 173 aa overlap with a predicted ORF (ORF 108.ng) from N gonorrhoeae: ml08/gl08 10 20 30 40 50 60 ml08. pep MLPGFNRIFKRFVPTLGTAHKTPPFALSRTGRLIRFYRHKRRGFNRKGIEMNKTLSILPV MMMMIMIMMMMMMIMMMMM MMMMMMMMMMMM gl08 MLPGFNRIFKRFAPTLGTAHKTPPFALSRTGRLIRSYRHKRRGFNRKGIEMNKTLSILPA 10 20 30 40 50 60 70 80 90 100 110 120 ml08. pep AILLGGCAAGGGNTFGSLDGGTGMGGSIVKMAVGSQCRAELDKRSEWRLTALAMSAEKQA M M M M M I M M M M M I I I I I I I I M 1 1 I I 1 1 I I I I I I I I I I gl08 AILLGGCAAGG-NTFGSLDGGTGMGGSIVKMTVESQCRAELDRRSEWRLTALAMSAEKQA 70 80 90 100 110 130 140 150 160 170 ml08. pep EWENKICACVAQEAPERMTGNDVMQMLAPSTRNQALAALTAKTVSACFKHLYRX 111 ! I 11 : 1 = = : I 11 : = : 11111 ! 111 I II II II II 11 M 11 II 111 M II gl08 EWENKICGCATEEAPNQLTGNDVMQMLNQSTRNQALAALTVKTVSACFKRLYRX 120 130 140 150 160 170 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 385>: alOβ.seq 1 ATGTTGCCGG GCTTCAACCG GATATTCAAA CGGTTTGTTC CAACACTCGG 51 AACGGCGCAT AAAACGCCGC CCTTCGCGTT ATCCCGAACG GGGCGGCTAA 101 TCAGATTCTA TCGCCATAAA AGGCGGGGTT TCAACCGAAA AGGAATTGAG 151 ATGAATAAAA CCTTGTCTAT TTTGCCGGTG GCAATCTTAC TCGGCGGCTG 201 CGCCGCCGGG GGCGGTAACA CATTCGGCAG CTTAGACGGC GGCACAGGTA 251 TGGGCGGCAG CATCGTCAAA ATGGCGGTAG AAAGCCAATG CCGTGCGGAA 301 TTGAACAAAC GCAGCGAATG GCGTTTGACC GCGCTGGCGA TGAGTGCCGA 351 AAAACAGGCG GAATGGGAAA ACAAGATTTG CGCTTGCGTC GCCCAAGAAG 401 CACCCAACCA GCTGACCGGC AACGATGTGA TGCAGATGCT GGATCCGTCC 451 ACGCGCAATC AGGCACTTGC CGCCCTGACC GCCAAAACGG TTTCCGCCTG 501 CTTCAAACAC CTGTACCGCT AA This corresponds to the amino acid sequence <SEQ ID 386; ORF 108.a>: alOβ.pep 1 MLPGFΝRIFK RFVPTLGTAH KTPPFALSRT GRLIRFYRHK RRGFΝRKGIE 51 MΝKTLSILPV AILLGGCAAG GGΝTFGSLDG GTGMGGSIVK MAVESQCRAE 101 LΝKRSEWRLT ALAMSAEKQA EWEΝKICACV AQEAPΝQLTG ΝDVMQMLDPS 151 TRΝQALAALT AKTVSACFKH LYR* ml08/al08 96.5% identity in 173 aa overlap 10 20 30 40 50 60 ml08.pep MLPGFΝRIFKRFVPTLGTAHKTPPFALSRTGRLIRFYRHKRRGFΝRKGIEMΝKTLSILPV I I I II I I I II II I I II II II I I II II II I II II II I I II I I II II I I I II I II I II I II I al08 MLPGFΝRIFKRFVPTLGTAHKTPPFALSRTGRLIRFYRHKRRGFΝRKGIEMΝKTLSILPV 10 20 30 40 50 60 70 80 90 100 110 120 ml08. pep AILLGGCAAGGGΝTFGSLDGGTGMGGSIVKMAVGSQCRAELDKRSEWRLTALAMSAEKQA I I I II I I I II II I I II II I I II II II II II I II II I II II : I I II I I II I II I II I II I al08 AILLGGCAAGGGΝTFGSLDGGTGMGGSIVKMAVESQCRAELΝKRSEWRLTALAMSAEKQA 70 80 90 100 110 120 130 140 150 160 170 ml08.pep EWEΝKICACVAQEAPERMTGΝDVMQMLAPSTRΝQALAALTAKTVSACFKHLYRX I II II II I I II II II ::: I I I II II I I II I I II II I II II II II I I I II I I II al08 EWEΝKICACVAQEAPΝQLTGΝDVMQMLDPSTRΝQALAALTAKTVSACFKHLYRX 130 140 150 160 170 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 387>: gl09. seq 1 ATGTATTATC GCCGGGTTGT GGGGCTATCC GATGGACTTG GCGATTTGGC 51 AGCCGGTATT GATCGTAGGC GTATGCTTAC CGCTTTTGGA AGCGGGCATG 101 GAAATGACGC GCAAAGGCAA AACCACCCAA TCCGCCGCCA TCGTGGTGTT 151 CTCTTCCGTC TGGTCAATCC GGTTTTCGGC TGGGCGTTGA CGATGCTGTT 201 GGATAATTTG GGCTTAATCG GCTGCAAAGA ACGCAGCGCG CAATTAGGTT 251 TTGTCGGACG AGTATTGATA CCCGCAGTAG GTTTCTTAAT CTTGTGTGTG 301 GCGATGGGTG CGGTCGGGAT GCTGCCCGGT ATCCCTCCGT TTTTGGAGCA 351 GTTCAAATCT TTGGGCTAG This corresponds to the amino acid sequence <SEQ ID 388; ORF 109.ng>: gl09.pep 1 MYYRRWGLS DGLGDLAAGI DRRRMLTAFG SGHGNDAQRQ NHPIRRHRGV 51 LFRLVNPVFG WALTMLLDNL GLIGCKERSA QLGFVGRV I PAVGF ILCV 101 AMGAVGMLPG IPPFLEQFKS LG The following partial DNA sequence was identified in N meningitidis <SEQ ID 389>: ml09. seq 1 ATGTATTATC GCCGGGTTAT GGGGCTATCC GATGGACTTG GCGATTTGGC 51 AGCCGGTATT GAGCGTAGCC TTGGTCGTAG GCGTATACTT ACCGCTTTTG 101 GAAGCGGGCA TGGAAATGAC GCGCAAAGGC AAAACCACCC AATCCGCCGC 151 CATCGTGGTG TTCTCTTCCG CCTTGTCAAT CCGGTTTTCG GCTGGGCGTT 201 GACGATGCTG TTGGATAATT TGGGCTTAAT CGGCTGCAAA GAGCGCAGTG 251 CGCAATTAGG TTTCGCCGGA CGCGTGTTGA TACCCGCAGT AGGTTTCTTG 301 ATCTTGTGTG TGGCGATGGG TGCGGTCGGG ATGCTGCCCG GTATCCCGCC 351 GTTTTTGGAA CACTTCAAAT CTTTGGGCTA G This corresponds to the amino acid sequence <SEQ ID 4; ORF 109>: ml09.pep 1 MYYRRVMGLS DGLGDLAAGI ERSLGRRRIL TAFGSGHGΝD AQRQΝHPIRR 51 HRGVLFRLVΝ PVFGWALTML LDΝLGLIGCK ERSAQLGFAG RVLIPAVGFL 101 ILCVAMGAVG MLPGIPPFLE HFKSLG* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 109 shows 92.9% identity over a 126 aa overlap with a predicted ORF (ORF 109.ng) from N gonorrhoeae: ml09/gl09 10 20 30 40 50 60 ml09 . pep MYYRRVMGLSDGLGDLAAGIERSLGRRRILTAFGSGHGΝDAQRQΝHPIRRHRGVLFRLVΝ M M M M M M M M M M M I I M 1 1 I I I I I I I I I 1 1 1 1 I I I I 1 1 1 1 1 I I I I I I gl09 MYYRRWGLSDGLGDLAAGIDR RRMLTAFGSGHGΝDAQRQΝHPIRRHRGVLFRLVΝ 10 20 30 40 50 70 80 90 100 110 120 ml09. pep PVFGWALTMLLDΝLGLIGCKERSAQLGFAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLE MIMMMMMMMMMMMMIMMMMMMMMMMMMMMMM gl09 PVFGWALTMLLDΝLGLIGCKERSAQLGFVGRVLIPAVGFLILCVAMGAVGMLPGIPPFLE 60 70 80 90 100 110 ml09.pep HFKSLGX . ■ M \ I iIlIlI gl09 QFKSLGX 120 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 391> : al09.seq 1 ATGTATTATC GCCGGGTTGT GGGGCTATCC GATGGACTTG GCGATTTGGC 51 AGCCGGTATT GAGCGTAGCC TTGGTCGTAG GCGTATACTT ACCGCTTTTG 101 GAAGCGGGCA TGGAAATGAC GCGCAAAGGC AAAACCACCC AATCCGCCGC 151 CACCGTGGTG TTCTCTTCCG CTTGGTCAAT CCGGTTTTCG GCTGGGCGTT 201 GACGATGCTG TTGGATAATT TGGGCTTAAT CGGCTGCAAA GAGCGCAGCG 251 CGCAATTAGG TTTCACCGGA CGCGTATTGA TACCCGTAGT AGGTTTCTTG 301 ATCTTGTGTG TGGCGATGGG TGCGGTCGGG ATGCTGCCCG GTATCCCGCC 351 GTTTTTGGAG CACTTCAAAT CTTTGGGCTA G This corresponds to the amino acid sequence <SEQ ID 392; ORF 109>: al09.pep 1 MYYRRVVGLS DGLGDLAAGI ERSLGRRRIL TAFGSGHGND AQRQNHPIRR 51 HRGVLFRLVN PVFGWALTML LDNLGLIGCK ERSAQLGFTG RVLIPWGFL 101 ILCVAMGAVG MLPGIPPFLE HFKSLG* ml09/al09 97.6% identity in 126 aa overlap 10 20 30 40 50 60 ml09.pep MYYRRVMGLSDGLGDLAAGIERSLGRRRILTAFGSGHGNDAQRQNHPIRRHRGVLFRLVN I I II I I : II II I II II II II I II I II II II I I II I II II I II II II I I I II I II II II I I al09 MYYRRVVGLSDGLGDLAAGIERSLGRRRILTAFGSGHGNDAQRQNHPIRRHRGVLFRLVN 10 20 30 40 50 60 70 80 90 100 110 120 ml09. pep PVFGWALTMLLDNLGLIGCKERSAQLGFAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLE II II I I II I II I II I II I II I II II II I : I I I II I: I II I II II II II II I II I II I II I al09 PVFGWALTMLLDNLGLIGCKERSAQLGFTGRVLIPVVGFLILCVAMGAVGMLPGIPPFLE 70 80 90 100 110 120 ml09. pep HFKSLGX al09 HFKSLGX The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 393>: gill. seq 1 ATGCCGTCTG AAACACGCCT GCCGAACCTT ATCCGCGCCT TGATATTTGC 51 CCTGGGTTTC ATCTTCCTGA ACGCCTGTTC GGaacaaacC GCGCAaaccg 101 TTACCCTGCA AGGCGAAACG ATGGGTACGA CCtATACCGT CAAATACCTT 151 TCAAATAATC GGGACAAACT CCCCTCCCCT GCCAAAATAC AAAAGCGCAT 201 TGATGATGCG CTTAAAGAAG TCAACCGGCA GATGtccaCC TACCAGACCG 251 ATTCCGAAAT CAGCCGGTTt atacagacan atgctggaga gctcttcgcg 301 tntcatgcag nttctataac tgattccgcc gaagactgtc tgcctaatac 351 gcctatctca tcggcgctct ga This corresponds to the amino acid sequence <SEQ ID 394; ORF 11 l.ng>: gill. pep 1 MPSETRLPΝL IRALIFALGF IFLΝACSEQT AQTVTLQGET MGTTYTVKYL 51 SΝΝRDKLPSP AKIQKRIDDA LKEVΝRQMST YQTDSEISRF IQTAGELFAH 101 ASITDSAEDC LPΝTPISSAL * The following partial DΝA sequence was identified in N meningitidis <SEQ ID 395>: mill . seq 1 ATGCCGTCTG AAACACGCCT GCCGAACTTT ATCCGCGTCT TGATATTTGC 51 CCTGGGTTTC ATCTTCCTGA ACGCCTGTTC GGAACAAACC GCGCAAACCG 101 TTACCCTGCA AGGCGAAACG ATGGGCACGA CCTATAyCGT CAAATACCTT 151 TCAAATAATC GGGACAAACT CCCCTCACCT GCCGAAATAC AwAAACGCAT 201 CGATGACGCG CTTAAAGAAk TCAACCGGyA GATGTCCACC TATCAGCCCG 251 ACTCCGAAAT CAGCCGGTTC AACCAACACA CAGCCGGCAA GCCCCTCCGC 301 ATTTCAAGCG ACTTCGCACA CGTTACTGCC GAAGCCGTCC GCCTGAACCG 351 CCTGACACAC GGCGCGCTGG ACGTAACCGT CGGCCCCTTG GTCAACCTTT 401 GGGGATTCGG CCCCGACAAA TCCGTTACCC GTGAACCGTC GCCGGAACAA 451 ATCAAACAGG CGGCATCTTA TACGGGCATA GACAAAATCA TTTTGAAACA 501 AGGCAAAGAT TACGCTTCCT TGAGCAAAAC CCACCCCAAG GCCTATTTGG 551 ATTTATCTTC GATTGCCAAA GGCTTCGGCG TTGATAAAGT TGCGGGCGAA 601 CTGGAAAAAT ACGGCATTCA AAATTATCTG GTCGAAATCG GCGGCGAGTT 651 GCACGGCAAA GGCAAAAACG CGCGCGGCGA ACCGTGGCGC ATCGGTATCG 701 AGCAGCCCAA TATCGTCCAA GGCGGCAATA CGCAGATTAT CGTCCCGCTG 751 AACAACCGTT CGCTTGCCAC TTCCGGCGAT TACCGTATTT TCCACGTCGA 801 TAAAAACGGC AAACGCCTCT CCCATATCAT CAACCCGAAC AACAAACGAC 851 CCATCAGCCA CAACCTCGCC TCCATCAGCG TGGTCGCAGA CAGTGCGATG 901 ACGGCGGACG GCTTGTCCAC AGGATTATTC GTATTGGGCG AAACCGAAGC 951 CTTAAAGCTG GCAGAGCGCG AAAAACTCGC TGTTTTCCTG ATTGTCAGGG 1001 ATAAAGGCGG cTACCGCACC GCCATGTCTT CCGAATTTGA AAAACTGcTC 1051 CGCTAA This corresponds to the amino acid sequence <SEQ ID 396; ORF 111>: mill. pep 1 MPSETRLPNF IRVLIFALGF IFLNACSEQT AQTVTLQGET MGTTYXVKYL 51 SNNRDKLPSP AEIXKRIDDA LKEXNRXMST YQPDSEISRF NQHTAGKPLR 101 ISSDFAHVTA EAVRLNRLTH GALDVTVGPL VNLWGFGPDK SVTREPSPEQ 151 IKQAASYTGI DKIILKQGKD YASLSKTHPK AYLDLSSIAK GFGVDKVAGE 201 LEKYGIQNYL VEIGGELHGK GKNARGEPWR IGIEQPNIVQ GGNTQ1IVPL 251 NNRSLATSGD YRIFHVDKNG KRLSHIINPN NKRPISHNLA SISWADSAM 301 TADGLSTGLF VLGETEALKL AEREKLAVFL IVRDKGGYRT AMSSEFEKLL 351 R* ORF 111 shows 88.7% identity over a 97 aa overlap with a predicted ORF (ORF 11 l.ng) from N gonorrhoeae: mill .pep/gill .pep 10 20 30 40 50 60 mill. ep MPSETRLPΝFIRVLIFALGFIFLΝACSEQTAQTVTLQGETMGTTYXVKYLSΝΝRDKLPSP MMMMIMIMMMMMMMMMMMMMMMMIMMMMIMMM gill MPSETRLPΝLIRALIFALGFIFLΝACSEQTAQTVTLQGETMGTTYTVKYLSΝΝRDKLPSP 10 20 30 40 50 60 70 80 90 100 110 120 mill .pep AEIXKRIDDALKEXΝRXMSTYQPDSEISRFΝQHTAGKPLRISSDFAHVTAEAVRLΝRLTH M l l l l l l l l l l I I M i l l I I I I I M I M M gill AKIQKRIDDALKEVΝRQMSTYQTDSEISRFIQTXAGELFAXHAXSITDSAEDCLPΝTPIS 70 80 90 100 110 120 130 140 150 160 170 180 mill . pep GALDVTVGPLVΝLWGFGPDKSVTREPSPEQIKQAASYTGIDKIILKQGKDYASLSKTHPK gill SALX The following partial DΝA sequence was identified in N meningitidis <SEQ ID 397>: alll . seq 1 ATGCCGTCTG AAACACGCCT GCCGAACTTT ATCCGCACCT TGATATTTGC 51 CCTGAGTTTT ATCTTCCTGA ACGCCTGTTC GGAACAAACC GCGCAAACCG 101 TTACCCTGCA AGGTGAAACG ATGGGCACGA CCTATACCGT CAAATACCTT 151 TCAAATAATC GGGACAAACT CCCCTCACCT GCCGAAATAC AAAAGCGCAT 201 CGATGACGCG CTTAAAGAAG TCAACCGGCA GATGTCCACC TATCAGCCCG 251 ACTCCGAAAT CAGCCGGTTC AACCAACACA CAGCCGGCAA GCCCCTCCGC 301 ATTTCAAGCG ACTTCGCACA CGTTACTGCC GAAGCCGTCC ACCTGAACCG 351 CCTGACACAC GGCGCGCTGG ACGTAACCGT CGGCCCCTTG GTCAACCTTT 401 GGGGATTCGG CCCCGACAAA TCCGTTACCC GTGAACCGTC GCCGGAACAA 451 ATCAAACAAG CAGCATCTTA TACGGGCATA GACAAAATCA TTTTGAAACA 501 AGGCAAAGAT TACGCTTCCT TGAGCAAAAC CCACCCCAAG GCCTATTTGG 551 ATTTATCTTC GATTGCCAAA GGCTTCGGCG TTGATAAAGT TGCGGGCGAA 601 CTGGAAAAAT ACGGCATTCA AAATTATCTG GTCGAAATCG GCGGCGAGTT 651 GCACGGCAAA GGCAAAAACG CGCGCGGCGA ACCTTGGCGC ATCGGCATCG 701 AACAGCCCAA CATCGTCCAA GGCGGCAATA CGCAGATTAT CGTCCCGCTG 751 AACAACCGTT CGCTTGCCAC TTCCGGCGAT TACCGTATTT TCCACGTCGA 801 TAAAAGCGGC AAACGCCTCT CCCATATCAT TAATCCGAAC AACAAACGAC 851 CCATCAGCCA CAACCTCGCC TCCATCAGCG TGGTCGCAGA CAGTGCGATG 901 ACGGCGGACG GCTTGTCCAC AGGATTATTC GTATTGGGCG AAACCGAAGC 951 CTTAAAGCTG GCAGAGCGCG AAAAACTCGC TGTTTTCCTG ATTGTCAGGG 1001 ATAAAGGCGG CTACCGCACC GCCATGTCTT CCGAATTTGA AAAACTGCTC 1051 CGCTAA This corresponds to the amino acid sequence <SEQ ID 398; ORF 11 l.a>: alll . pep 1 MPSETRLPNF IRTLIFALSF IFLNACSEQT AQTVTLQGET MGTTYTVKYL 51 SNNRDKLPSP AEIQKRIDDA LKEVNRQMST YQPDSEISRF NQHTAGKPLR 101 ISSDFAHVTA EAVHLNRLTH GALDVTVGPL VNLWGFGPDK SVTREPSPEQ 151 IKQAASYTGI DKIILKQGKD YASLSKTHPK AYLDLSSIAK GFGVDKVAGE 201 LEKYGIQNYL VEIGGELHGK GKNARGEPWR IGIEQPNIVQ GGNTQIIVPL 251 NNRSLATSGD YRIFHVDKSG KRLSHIINPN NKRPISHNLA SISWADSAM 301 TADGLSTGLF VLGETEALKL AEREKLAVFL IVRDKGGYRT AMSSEFEKLL 351 R* mlll/alll 97.7% identity in 351 aa overlap 10 20 30 40 50 60 mill. pep MPSETRLPNFIRVLIFALGFIFLNACSEQTAQTVTLQGETMGTTYXVKYLSNNRDKLPSP II I II II II II I : I II II : II I I II I II II II II I II I II II II I : II I I II I II I I II I alll MPSETRLPNFIRTLIFALSFIFLNACSEQTAQTVTLQGETMGTTYTVKYLSNNRDKLPSP 10 20 30 40 50 60 70 80 90 100 110 120 ml11. pep AEIXKRIDDALKEXNRXMSTYQPDSEISRFNQHTAGKPLRISSDFAHVTAEAVRLNRLTH I I I l l l l l l l l l I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I: I I I I I I alll AEIQKRIDDALKEVNRQMSTYQPDSEISRFNQHTAGKPLRISSDFAHVTAEAVHLNRLTH 70 80 90 100 110 120 130 140 150 160 170 180 ml 11. pep GALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGIDKIILKQGKDYASLSKTHPK II I I II II I II I II I II II II I II II II II II II I II I II II II II II I I II I I I II II I alll GALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGIDKIILKQGKDYASLSKTHPK 130 140 150 160 170 180 190 200 210 220 230 240 mill. pep AYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELHGKGKNARGEPWRIGIEQPNIVQ II I II II II I I I II II I I II II II II I II II II I II II II I I II II I I I I II I II II II I alll AYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELHGKGKNARGEPWRIGIEQPNIVQ 190 200 210 220 230 240 250 260 270 280 290 300 mill. pep GGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHIINPNNKRPISHNLASISWADSAM MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM alll GGNTQIIVPLNNRSLATSGDYRIFHVDKSGKRLSHIINPNNKRPISHNLASISWADSAM 250 260 270 280 290 300 310 320 330 340 350 mill .pep TADGLSTGLFVLGETEALKLAEREKLAVFLIVRDKGGYRTAMSSEFEKLLRX II II II II I II II I I II II II I I II I II II I I I II II I II I II II II II II I alll TADGLSTGLFVLGETEALKLAEREKLAVFLIVRDKGGYRTAMSSEFEKLLRX 310 320 330 340 350 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 399>: glll-l.seq 1 ATGCCGTCTG AAACACGCCT GCCGAACCTT ATCCGCGCCT TGATATTTGC 51 CCTGGGTTTC ATCTTCCTGA ACGCCTGTTC GGAACAAACC GCGCAAacCG 101 TTACCCTGCA AGGCGAAACG ATGGGTACGA CCTATACCGT CAAATACCTT 151 TCAAATAATC GGGACAAACT CCCCTCCCCT GCCAAAATAC AAAAGCGCAT 201 TGATGATGCG CTTAAAGAAG TCAACCGGCA GATGTCCACC TACCAGACCG 251 ATTCCGAAAT CAGCCGGTTC AACCAACACA CAGCCGGCAA GCCCCTCCGC 301 ATTTCAAGCG ATTTCGCACA CGTTACCGCC GAAGCCGTCC GCCTGAACCG 351 CCTGACTCAC GGCGCACTGG ACGTAACCGT CGGCCCTTTG GTCAACCTTT 401 GGGGGTTCGG CCCCGACAAA TCCGTTACCC GTGAACCGTC GCCGGAACAA 451 ATCAAACAGG CGGCATCTTA TACGGGCATA GACAAAATCA TTTTGCAACA 501 AGGCAAAGAT TACGCTTCCT TGAGCAAAAC CCACCCCAAA GCCTATTTGG 551 ATTTATCTTC GATTGCCAAA GGCTTCGGCG TTGATAAAGT TGCGGGCGAA 601 CTGGAAAAAT ACGGCATTCA AAATTATCTG GTCGAAATCG GCggcGAGTT 651 GCACGGCAAA GGCAAAAATG CGCACGGCGA ACCGTGGCGC ATCGGTATAG 701 AGCAACCCAA TATcatccaa ggcggcaata cgcAGattat cgtcccgctg 751 aaCaaccgtt cgcttgccac ttccggcgAT taccgtaTTT tccacgtcgA 801 TAAAAACGGC Aaacgccttt cccacATCAT CAATCCCAAC AACAAACGAC 851 CCATCAGCCA CAAcctcgcc tcCATCAgCg TGGTCTCAGA CAGTGCAATG 901 ACGGCGGACG GTTTATCCAC AGGATTATTT GTTTTAGGCG AAACCGAAGC 951 CTTAAGGCTG GCAGAACAAG AAAAACTCGC TGTTTTCCTA ATTGTCCGGG 1001 ATAAGGACGG CTACCGCACC GCCATGTCTT CCGAATTTGC CAAGCTGCTC 1051 CGCTAA This corresponds to the amino acid sequence <SEQ ID 400; ORF 11 l-l.ng>: glll-l.pep 1 MPSETRLPNL IRALIFALGF IFLNACSEQT AQTVTLQGET MGTTYTVKYL 51 SNNRDKLPSP AKIQKRIDDA LKEVNRQMST YQTDSEISRF NQHTAGKPLR 101 ISSDFAHVTA EAVRLNRLTH GALDVTVGPL VNLWGFGPDK SVTREPSPEQ 151 IKQAASYTGI DKIILQQGKD YASLSKTHPK AYLDLSSIAK GFGVDKVAGE 201 LEKYGIQNYL VEIGGELHGK GKNAHGEPWR IGIEQPNIIQ GGNTQIIVPL 251 NNRSLATSGD YRIFHVDKNG KRLSHIINPN NKRPISHNLA SISVVSDSAM 301 TADGLSTGLF VLGETEALRL AEQEKLAVFL IVRDKDGYRT AMSΞEFAKLL 351 R* The following partial DNA sequence was identified in N meningitidis <SEQ ID 401>: lll-l.seq 1 ATGCCGTCTG AAACACGCCT GCCGAACTTT ATCCGCGTCT TGATATTTGC 51 CCTGGGTTTC ATCTTCCTGA ACGCCTGTTC GGAACAAACC GCGCAAACCG 101 TTACCCTGCA AGGCGAAACG ATGGGCACGA CCTATACCGT CAAATACCTT 151 TCAAATAATC GGGACAAACT CCCCTCACCT GCCGAAATAC AAAAACGCAT 201 CGATGACGCG CTTAAAGAAG TCAACCGGCA GATGTCCACC TATCAGCCCG 251 ACTCCGAAAT CAGCCGGTTC AACCAACACA CAGCCGGCAA GCCCCTCCGC 301 ATTTCAAGCG ACTTCGCACA CGTTACTGCC GAAGCCGTCC GCCTGAACCG 351 CCTGACACAC GGCGCGCTGG ACGTAACCGT CGGCCCCTTG GTCAACCTTT 401 GGGGATTCGG CCCCGACAAA TCCGTTACCC GTGAACCGTC GCCGGAACAA 451 ATCAAACAGG CGGCATCTTA TACGGGCATA GACAAAATCA TTTTGAAACA 501 AGGCAAAGAT TACGCTTCCT TGAGCAAAAC CCACCCCAAG GCCTATTTGG 551 ATTTATCTTC GATTGCCAAA GGCTTCGGCG TTGATAAAGT TGCGGGCGAA 601 CTGGAAAAAT ACGGCATTCA AAATTATCTG GTCGAAATCG GCGGCGAGTT 651 GCACGGCAAA GGCAAAAACG CGCGCGGCGA ACCGTGGCGC ATCGGTATCG 701 AGCAGCCCAA TATCGTCCAA GGCGGCAATA CGCAGATTAT CGTCCCGCTG 751 AACAACCGTT CGCTTGCCAC TTCCGGCGAT TACCGTATTT TCCACGTCGA 801 TAAAAACGGC AAACGCCTCT CCCATATCAT CAACCCGAAC AACAAACGAC 851 CCATCAGCCA CAACCTCGCC TCCATCAGCG TGGTCGCAGA CAGTGCGATG 901 ACGGCGGACG GCTTGTCCAC AGGATTATTC GTATTGGGCG AAACCGAAGC 951 CTTAAAGCTG GCAGAGCGCG AAAAACTCGC TGTTTTCCTG ATTGTCAGGG 1001 ATAAAGGCGG CTACCGCACC GCCATGTCTT CCGAATTTGA AAAACTGCTC 1051 CGCTAA This corresponds to the amino acid sequence <SEQ ID 402; ORF 111-1 > • mlll-l.pep 1 MPSETRLPNF IRVLIFALGF IFLNACSEQT AQTVTLQGET MGTTYTVKYL 51 SNNRDKLPSP AEIQKRIDDA LKEVNRQMST YQPDSEISRF NQHTAGKPLR 101 ISSDFAHVTA EAVRLNRLTH GALDVTVGPL VNLWGFGPDK SVTREPSPEQ 151 IKQAASYTGI DKIILKQGKD YASLSKTHPK AYLDLSSIAK GFGVDKVAGE 201 LEKYGIQNYL VEIGGELHGK GKNARGEPWR IGIEQPNIVQ GGNTQIIVPL 251 NNRSLATSGD YRIFHVDKNG KRLSHIINPN NKRPISHNLA SISWADSAM 301 TADGLSTGLF VLGETEALKL AEREKLAVFL IVRDKGGYRT AMSSEFEKLL 351 R* mlll-l/glll-1 96.6% identity in 351 aa overlap 10 20 30 40 50 60 mlll-l.pep MPSETRLPNFIRVLIFALGFIFLNACSEQTAQTVTLQGETMGTTYTVKYLSNNRDKLPΞP I I II I I I I hi I :l I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I glll-1 MPSETRLPNLIRALIFALGFIFLNACSEQTAQTVTLQGETMGTTYTVKYLSNNRDKLPSP 10 20 30 40 50 60 70 80 90 100 110 120 mlll-l.pep AEIQKRIDDALKEVNRQMSTYQPDSEISRFNQHTAGKPLRISSDFAHVTAEAVRLNRLTH I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I glll-1 AKIQKRIDDALKEVNRQMSTYQTDSEISRFNQHTAGKPLRISSDFAHVTAEAVRLNRLTH 70 80 90 100 110 120 130 140 150 160 170 180 mlll-l.pep GALDVTVGPLVNLWGFGPDKΞVTREPSPEQIKQAASYTGIDKIILKQGKDYASLSKTHPK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I :| I I I I I I I I I I I I I glll-1 GALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAAΞYTGIDKIILQQGKDYASLSKTHPK 130 140 150 160 170 180 190 200 210 220 230 240 ml11-1. pep AYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELHGKGKNARGEPWRIGIEQPNIVQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I :l I I I I I I I I I I I I : I glll-1 AYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELHGKGKNAHGEPWRIGIEQPNIIQ 190 200 210 220 230 240 250 260 270 280 290 300 mlll-l.pep GGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHIINPNNKRPISHNLASISVVADSAM I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I :| I I I glll-1 GGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHIINPNNKRPISHNLASISVVSDSAM 250 260 270 280 290 300 310 320 330 340 350 mlll-1.pep TADGLSTGLFVLGETEALKLAEREKLAVFLIVRDKGGYRTAMSSEFEKLLRX I I I I I I I I I I I I I I I I I I :| I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I glll-1 TADGLSTGLFVLGETEALRLAEQEKLAVFLIVRDKDGYRTAMSΞEFAKLLRX 310 320 330 340 350 glll-l/p44550 sp|P44550| YOJL_HAEIN HYPOTHETICAL LIPOPROTEIN HI0172 PRECURSOR >gι | 1074292 | pir | | C64144 hypothetical protein HI0172 - Haemophilus influenzae (strain Rd KW20) >gι 11573128 (032702) lipoprotein, putative [Haemophilus influenzae Rd] Length = 346 Score = 349 bits (885), Expect = 2e-95 Identities = 177/328 (53%), Positives = 240/328 (72%), Gaps = 4/328 (1%) Query: 23 LNACSEQTAQTVTLQGETMGTTYXVKYLSNNRDKLPSPAEIXKRIDDALKEXNRXMSTYQ 82 L AC ++T + ++L G+TMGTTY VKYL + S + + 1+ LK+ N MSTY+ Sbjct: 17 LAACQKET-KVISLSGKTMGTTYHVKYLDDGΞITATS-EKTHEEIEAILKDVNAKMSTYK 74 Query: 83 PDSEISRFNQHT-AGKPLRISSDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKS 141 DSE+SRFNQ+T P+ IS+DFA V AEA+RLN++T GALDVTVGP+VNLWGFGP+K Sbjct: 75 KDSELΞRFNQNTQVNTPIEISADFAKVLAEAIRLNKVTEGALDVTVGPVVNLWGFGPEKR 134 Query: 142 VTREPSPEQIKQAASYTGIDKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGEL 201 ++P+PEQ+ + ++ GIDKI L K+ A+LSK P+ Y+DLSSIAKGFGVD+VA +L Sbjct: 135 PEKQPTPEQLAERQAWVGIDKITLDTNKEKATLSKALPQVYVDLSSIAKGFGVDQVAEKL 194 Query: 202 EKYGIQNYLVEIGGELHGKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDY 261 E+ QNY+VEIGGE+ KGKN G+PW+I IE+P + ++ LNN +A+ΞGDY Sbjct: 195 EQLNAQNYMVEIGGEIRAKGKNIEGKPWQIAIEKPTTTGERAVEAVIGLNNMGMAΞSGDY 254 Query: 262 RIFHVDKNGKRLΞHIINPNNKRPISHNLASIΞVVADSAMTADGLΞTGLFVLGETEALKLA 321 RI+ ++NGKR +H I+P PI H+LASI+V+A ++MTADGLSTGLFVLGE +AL++A Sbjct: 255 RIY-FEENGKRFAHEIDPKTGYPIQHHLASITVLAPTSMTADGLSTGLFVLGEDKALEVA 313 Query: 322 EREKLAVFLIVRDKGGYRTAMSSEFEKL 349 E+ LAV+LI+R G+ T SS F+KL Sbjct: 314 EKNNLAVYLIIRTDNGFVTKSSSAFKKL 341 The following partial DNA sequence was identified in N meningitidis <SEQ ID 403>: alll-l .seq 1 ATGCCGTCTG AAACACGCCT GCCGAACTTT ATCCGCACCT TGATATTTGC 51 CCTGAGTTTT ATCTTCCTGA ACGCCTGTTC GGAACAAACC GCGCAAACCG 101 TTACCCTGCA AGGTGAAACG ATGGGCACGA CCTATACCGT CAAATACCTT 151 TCAAATAATC GGGACAAACT CCCCTCACCT GCCGAAATAC AAAAGCGCAT 201 CGATGACGCG CTTAAAGAAG TCAACCGGCA GATGTCCACC TATCAGCCCG 251 ACTCCGAAAT CAGCCGGTTC AACCAACACA CAGCCGGCAA GCCCCTCCGC 301 ATTTCAAGCG ACTTCGCACA CGTTACTGCC GAAGCCGTCC ACCTGAACCG 351 CCTGACACAC GGCGCGCTGG ACGTAACCGT CGGCCCCTTG GTCAACCTTT 401 GGGGATTCGG CCCCGACAAA TCCGTTACCC GTGAACCGTC GCCGGAACAA 451 ATCAAACAAG CAGCATCTTA TACGGGCATA GACAAAATCA TTTTGAAACA 501 AGGCAAAGAT TACGCTTCCT TGAGCAAAAC CCACCCCAAG GCCTATTTGG 551 ATTTATCTTC GATTGCCAAA GGCTTCGGCG TTGATAAAGT TGCGGGCGAA 601 CTGGAAAAAT ACGGCATTCA AAATTATCTG GTCGAAATCG GCGGCGAGTT 651 GCACGGCAAA GGCAAAAACG CGCGCGGCGA ACCTTGGCGC ATCGGCATCG 701 AACAGCCCAA CATCGTCCAA GGCGGCAATA CGCAGATTAT CGTCCCGCTG 751 AACAACCGTT CGCTTGCCAC TTCCGGCGAT TACCGTATTT TCCACGTCGA 801 TAAAAGCGGC AAACGCCTCT CCCATATCAT TAATCCGAAC AACAAACGAC 851 CCATCAGCCA CAACCTCGCC TCCATCAGCG TGGTCGCAGA CAGTGCGATG 901 ACGGCGGACG GCTTGTCCAC AGGATTATTC GTATTGGGCG AAACCGAAGC 951 CTTAAAGCTG GCAGAGCGCG AAAAACTCGC TGTTTTCCTG ATTGTCAGGG 001 ATAAAGGCGG CTACCGCACC GCCATGTCTT CCGAATTTGA AAAACTGCTC 051 CGCTAA This corresponds to the amino acid sequence <SEQ ID 404; ORF 111-1. a>: alll-l.pep 1 MPSETRLPNF IRTLIFALSF IFLNACSEQT AQTVTLQGET MGTTYTVKYL 51 SNNRDKLPSP AEIQKRIDDA LKEVNRQMST YQPDSEISRF NQHTAGKPLR 101 ISSDFAHVTA EAVHLNRLTH GALDVTVGPL VNLWGFGPDK SVTREPSPEQ 151 IKQAASYTGI DKIILKQGKD YASLSKTHPK AYLDLSSIAK GFGVDKVAGE 201 LEKYGIQNYL VEIGGELHGK GKNARGEPWR IGIEQPNIVQ GGNTQIIVPL 251 NNRSLATSGD YRIFHVDKSG KRLSHIINPN NKRPISHNLA SISWADSAM 301 TADGLSTGLF VLGETEALKL AEREKLAVFL IVRDKGGYRT AMSSEFEKLL 351 R* alll-l/mlll-1 98.9% identity in 351 aa overlap 10 20 30 40 50 60 alll-l.pep MPSETRLPNFIRTLIFALSFIFLNACΞEQTAQTVTLQGETMGTTYTVKYLΞNNRDKLPSP I I I I I I I I I I I I : I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I mlli-i MPSETRLPNFIRVLIFALGFIFLNACSEQTAQTVTLQGETMGTTYTVKYLSNNRDKLPΞP 10 20 30 40 50 60 70 80 90 100 110 120 alll-l.pep AEIQKRIDDALKEVNRQMSTYQPDSEISRFNQHTAGKPLRISSDFAHVTAEAVHLNRLTH I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I mlll-i AEIQKRIDDALKEVNRQMSTYQPDSEISRFNQHTAGKPLRISSDFAHVTAEAVRLNRLTH 70 80 90 100 110 120 130 140 150 160 170 180 alll-l.pep GALDVTVGPLVNLWGFGPDKSVTREPΞPEQIKQAASYTGIDKIILKQGKDYASLSKTHPK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I mlll-1 GALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGIDKIILKQGKDYASLSKTHPK 130 140 150 160 170 180 190 200 210 220 230 240 alll-l.pep AYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELHGKGKNARGEPWRIGIEQPNIVQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I mlll-1 AYLDLΞΞIAKGFGVDKVAGELEKYGIQNYLVEIGGELHGKGKNARGEPWRIGIEQPNIVQ 190 200 210 220 230 240 250 260 270 280 290 300 alll-l.pep GGNTQIIVPLNNRSLATSGDYRIFHVDKΞGKRLΞHIINPNNKRPISHNLASISVVADSAM I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I mlll-1 GGNTQIIVPLNNRΞLATSGDYRIFHVDKNGKRLSHIINPNNKRPISHNLASISWADSAM 250 260 270 280 290 300 310 320 330 340 350 alll-1.pep TADGLSTGLFVLGETEALKLAEREKLAVFLIVRDKGGYRTAMSSEFEKLLRX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml11-1 TADGLSTGLFVLGETEALKLAEREKLAVFLIVRDKGGYRTAMSSEFEKLLRX 310 320 330 340 350 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 405>: gll4. seq 1 ATGGCTTCCA TCACTTCGCC GCTGCACGGG GCGCAGCAGG AATGCAGCAA 51 GACTTTTTTA TGTCCGCCGG GCGGGACGAG TATGGGGCGG TCAATGTCGG 101 TAACGGTAGG TTTGTTTTGT GTTTCCATTA ACTTAACAAT ATCTGTCGAA 151 TACGGTCAAA GCGGCTATTT TACCAGAGCC GCCGAATGTA AAACAGGGTG 201 TCAGGGCATC AGCCCGAGCT GCCTGAACGA ACGGACGGTT TGCGAGGTAA 251 CGATAAAATG GTCGAGCAGC GAAACATCAA CCAGCGACAT GGCCTGTGCC 301 AGCCGCCTTG TGAACATGAT GTCTTCCTGC GAAGGTTCAG GCGAGCCGCC 351 CGGATGGTTG TGCGCGATAA TCAGGCTGTC GGCATATTCG TCCAATGCCA 401 GTTTGACGAT TTCGCGGATG TAA This corresponds to the amino acid sequence <SEQ ID 406; ORF 114.ng>: gll4.pep 1 MASITSPLHG AQQECSKTFL CPPGGTSMGR SMSVTVGLFC VSINLTISVE 51 YGQSGYFTRA AECKTGCQGI SPSCLNERTV CEVTIKWSSS ETSTSDMACA 101 SRLVNMMSSC EGSGEPPGWL CAIIRLSAYS SNASLTISRM * The following partial DNA sequence was identified in N meningitidis <SEQ ID 407>: mll4. seq 1 ATGGCTTCCA TCACTTCGCC GCTGCACGGG GCGCACAGAG AATGCAGCAA 51 GACTTTTTTA TGTCCACCGG GCGGGACGAG TATAGGGCGG TCAATGTCGG 101 TAACGGTAGG TTTGTTTTGT GTTTCCATTA ACTTAACAAT ATCTGTTGAA 151 TACGGTTGAA GCGGCTATTT TATCAGAGCC GCCGCATGTA AAACAGAGTG 201 TCAGGGCATC AACCCGAGCT GTCTGAACGA ACAGACGCTT TGCGA GTAA 251 CGATAAAATG GTCGAGCAGC GACACATCGA CCAGCGACAT TGCCTGTGCC 301 AGCCGCCTTG TGAACATGAT GTCTTCCTGC GAArGTTCsG GCGAGCCGcC 351 CGgATGGTTG TGCGCAATAA TCAGGCTGTC GGCATATTCG TCCAATGCCA 401 GTTTGACGAT TTCGCGGATG TAA This corresponds to the amino acid sequence <SEQ ID 408; ORF 114>: mll4.pep 1 MASITSPLHG AHRECSKTFL CPPGGTSIGR SMSVTVGLFC VSINLTISVE 51 YGXSGYFIRA AACKTECQGI NPSCLNEQTL CXVTIKWSSS DTSTSDIACA 101 SRLVNMMSSC EXSGEPPGWL CAIIRLSAYS SNASLTISRM * mll4/gll4 90.0% identity over a 140 aa overlap 10 20 30 40 50 60 mll4.pep MASITSPLHGAHRECSKTFLCPPGGTSIGRSMSVTVGLFCVSINLTISVEYGXSGYFIRA M i l I I gll4 MASITSPLHGAQQECSKTFLCPPGGTSMGRSMSVTVGLFCVSINLTISVEYGQSGYFTRA 10 20 30 40 50 60 70 80 90 100 110 120 mll4. pep AACKTECQGINPSCLNEQTLCXVTIKWSSSDTSTSDIACASRLVNMMSSCEXSGEPPGWL I I I I l l l l l l l l l gll AECKTGCQGISPSCLNERTVCEVTIKWSSSETSTSDMACASRLVNMMSSCEGSGEPPGWL 70 80 90 100 110 120 130 140 mll4.pep CAIIRLSAYSSNASLTISRMX gll4 CAIIRLSAYSSNASLTISRMX 130 140 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 409> all4. seq 1 ATGCCGGAGG CAAGCATCGC CTCCATCACT TCGCCGCTGC ACGGGGCGCA 51 ACAGGAATGC AGCAAGACTT TTTTATGTCC GCCGGGCGGG ACGAGTATGG 101 GGCGGTCAAT GTCGGTAACG GTAGGTTTGT TTTGTGTTTC CATTAACTTA 151 ACGATATCTG TCGAATACGG TTGAAGCGGC TATTTTATCA GAGCCGCCGC 201 ATGTAAAACA GGGTGTCAGG GCATCAGCCC GAGCTGCCTG AACGAACGGA 251 CGGTTTGCGC CGTTACGATA AAATGGTCGA GCAGCGACAC ATCGACCAGC 301 GACATTGCCT GTGCCAGCCG CCTTGTGAAC ATGATGTCTT CCTGCGAAGG 351 TTCGGGCGAG CCGCCCGGAT GGTTGTGCGC GATAATCAGG CTGTCGGCAT 401 ATTCGTCCAA TGCCAGTTTG ACAATTTCAC GGATGTAA This corresponds to the amino acid sequence <SEQ ID 410; ORF 114.a>: all4.pep 1 MPEASIASIT SPLHGAQQEC SKTFLCPPGG TSMGRSMSVT VGLFCVSINL 51 TISVEYG*SG YFIRAAACKT GCQGISPSCL NERTVCAVTI KWSSSDTSTS 101 DIACASRLVN MMSSCEGSGE PPGWLCAIIR LSAYSSNASL TISRM* ll4/all4 92.9% identity in 140 aa overlap 10 20 30 40 50 mll4.pep MASITSPLHGAHRECSKTFLCPPGGTSIGRSMSVTVGLFCVSINLTISVEYGXSG : I I I I I I I I I I :: I I I II I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I II II I all4 MPEASIASITSPLHGAQQECSKTFLCPPGGTSMGRSMSVTVGLFCVSINLTISVEYGXSG 10 20 30 40 50 60 60 70 80 90 100 110 mll4.pep YFIRAAACKTECQGINPSCLNEQTLCXVTIKWSSSDTSTSDIACASRLVNMMSSCEXSGE I II I I I I I I I I I I I : I I I I I I : I : I I I I II I I I I I I I I I I I I I I I I I I I I I I I I III all4 YFIRAAACKTGCQGISPSCLNERTVCAVTIKWSSSDTSTSDIACASRLVNMMSSCEGSGE 70 80 90 100 110 120 120 130 140 ml14.pep PPGWLCAIIRLSAYSSNASLTISRMX II I I I I I I I I I I I I I I I I I I I I I I I I all4 PPGWLCAIIRLSAYSSNASLTISRMX 130 140 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 411>: gll7.seq 1 atggtcgacg aactcgacCT GCTGCCCGAT GCCGTCGCCG CCACCCTGCT 51 TGCCGACATC GGACGCTACG TCCCCGATTG GAACCTATTG GTTTCCGAGC 101 GCTGCAACAG CACCGTCGCC GAGCTGGTCA AAGGTGtgga CGAAGTGCAG 151 AAACTTACCC ACTTCGCCCG GGTGGACAGC CTCGCCACGC CGGAAGAACG 201 CGCACAGCAA GCGGAAACCA TGCGGAAAAT GCTGCTGGCg atggttaccg 251 Acatccgcgt cgtaTTAATC AAACTGGCGA TGCGTacgcg caccCTGcta 301 ttTTtaaGCA ACGCCCCCGA CAGCCCTGAA AAACgcgccG TCgccaaAga 351 aacccTCGAC ATCTTCGCCC CGCTCGCCAA CCGCTTGGGC GTGTGGCAGC 401 TCAAATGGCA GCTCGAAGAT TTGGGCTTCC GCCATCAAGA ACCCGAAAAA 451 TACCGCGAAA TCGCCCTGCT TTTGGACGAA AAACGCACCG AACGCCTCGA 501 ATACATCGAA AACTTCCTCG ATATCCTGCG TACGGAACTC AAAAAATACA 551 ATATCCACTT TGAAGTCGCC GGCCGTCCGA AACACATCTA CTCCATTTAC 601 AAAAAAATGG TGAAGAAAAA ACTCAGCTTC GACGgccTGT TCGACATCCG 651 CGCCGTGCGG ATTCTGGTCG ATACCGTCCC CGaGTGTTAC ACCACGCTGG 701 gcaTCGTCCA CAGCCTCTGG CAGCCCATTC CCGGCGagtt CGAcgactAC 751 ATCGCCAACC CCAAAGgcaA CGgttATAAA AGtTTGCACA CCGTCATCGT 801 cggcccGGAa gacaaaggtg tggaaGtgCA AATCCGCACC TTCGAtatGC 851 accAATTCaa CgaatTcggT gtcgccgCCC ACTGGCGtta caaagaaggc 901 ggcaaaggcg attccGCCtA cgaacaaAAA ATcgccTggt TGCgccaACT 951 CTTGGACTGG CGCGAAAATA TGGCGGAAAG CGGCAAGGAA GACCTCGCCG 1001 CCGCCTTCAA AACCGAGCTT TTCAACGACA CGATTTATGT TTTGACCCCG 1051 CACGGCAAAG TCCTCTCTCT GCCAACGGGC GCAACCCCCA TCGACTTCGC 1101 CTACGCCCTG CACAGCAGCA TcggCGACCG CTGCCGGGGC GCGAAAGTCG 1151 AaggGCAGAT TGTGCCGCTG TCCACCCCGC TCGAAAACGG ACAGCGCGTC 1201 GAAATcatta cCGCcaaAGA AGGGCATCCT TCCGTCAACT GGCTTTACGA 1251 AGGctgGGtc aAATCCGGCA AGGCCATCGG caaAATCCGC GCCTAcatCC 1301 GCCAGcaaAa cgCcgaCACC GTGCGCGAAG AAGGCCGTGT CCAACTCGAC 1351 AAGCAGCTTG CCAAACTCAC GCCCAAACCC AACCTGCAAG AGCTTgccga 1401 aaATCTCGGC tacaaAAAGC cagaagacct ctacacCGCc gtcggacaag 1451 gcgaaatttc caaccgcgcc atCcaaaaag cctgcggcac GCTgaacgaa 1501 ccgccccCCG TGCCCGTCAG CGCAACCACC ATCGTCAAAC AGTCCAAAAT 1551 CAAAAAAGGT GGCAAAACCG GCGTGCTCAT CGACGGCGAA GACGGCTTGA 1601 TGACCACGCT TGCCAAATGC TGCAAACCCG CGCCGCCCGA CGATATTGCC 1651 GGCTTCGTTA CCCGCGAGCG CGGCATTTCC GTCCACCGCA AAACCTGCCC 1701 CTCTTTCCGA CACCTTGCCG AACACGCGCC CGAAAAAGTA CTGGACGCAA 1751 GTTGGGCGGC GTTGCAGGAA GGGCAAGTGT TCGCCGTCGA TATCGAAATC 1801 CGCGCCCAAG ACCGCTCCGG GCTTTTGCGC GACGTATCCG ACGCGCTCGC 1851 CCGCCACAAA CTCAACGTTA CCGCCGTGCA AACCCAGTCC CGCGACTTGG 1901 AAGCCAGCAT GAGGTTCACG CTCGAAGTCA AACAAGtCAA CGacCTCCCG 1951 CGCGTCCTCG CCGGCCTCGG CGATGTCAAA GGCGTATTGA GCGTTACCCG 2001 GCTTTAA This corresponds to the amino acid sequence <SEQ ID 412; ORF 117.ng>: gll7.pep 1 MVDELDLLPD AVAATLLADI GRYVPDWNLL VSERCNSTVA ELVKGVDEVQ 51 KLTHFARVDS LATPEERAQQ AETMRKMLLA MVTDIRWLI KLAMRTRTLL 101 FLSNAPDSPE KRAVAKETLD IFAPLANRLG VWQLKWQLED LGFRHQEPEK 151 YREIALLLDE KRTERLEYIE NFLDILRTEL KKYNIHFEVA GRPKHIYSIY 201 KKMVKKKLSF DGLFDIRAVR ILVDTVPECY TTLGIVHSLW QPIPGEFDDY 251 IANPKGNGYK SLHTVIVGPE DKGVEVQIRT FDMHQFNEFG VAAHWRYKEG 301 GKGDSAYEQK IAWLRQLLDW RENMAESGKE DLAAAFKTEL FNDTIYVLTP 351 HGKVLSLPTG ATPIDFAYAL HSSIGDRCRG AKVEGQIVPL STPLENGQRV 401 EIITAKEGHP SVNWLYEGWV KSGKAIGKIR AYIRQQNADT VREEGRVQLD 451 KQLAKLTPKP NLQELAENLG YKKPEDLYTA VGQGEISNRA IQKACGTLNE 501 PPPVPVSATT IVKQSKIKKG GKTGVLIDGE DGLMTTLAKC CKPAPPDDIA 551 GFVTRERGIS VHRKTCPSFR HLAEHAPEKV LDASWAALQE GQVFAVDIEI 601 RAQDRSGLLR DVSDALARHK LNVTAVQTQS RDLEASMRFT LEVKQVNDLP 651 RVLAGLGDVK GVLSVTRL* The following partial DNA sequence was identified in N meningitidis <SEQ ID 413>: mll7.seq (partial) 1 .. GTGAAACTCA AGAAATACAA TGTCCATTTC GAAGTCGCCG GCCGCCCGAA 51 ACACATCTAC TCCATTTACA AAAAAATGGT GAAGAAAAAA CTCAGCTTCG 101 ACGGCCTCTT TGACATCCGC GCCGTGCGAA TTCTGGTTGA TACCGTCCCC 151 GAGTGTTACA CCACGCTGGG TATCGTCCAC AGCCTCTGGC AGCCCATTCC 201 CGGCGAGTTC GACGACTACA TCGCCAATCC CAAAGGCAAC GGCTATAAAA 251 GTTTGCACAC CGTCATCGTC GGCCCGGAAG ACAAAGGCGT GGAAGTACAA 301 ATCCGCACCT TCGATATGCA CCAATTCAAC GAATTCGGTG TCGCCGCCCA 351 CTGgCGTTAC AAAGAGGGCG GCAAGGGCGA TTCCGCCTAC GAACAGAAAA 401 TCGCCTGGTT GCGCCAACTC TTGGACTGGC GCGAAAACAT GGCGGAAAGC 451 GGCAAGGAAG ACCTCGCCGC CGCCTTCAAA ACCGAGCTTT TCAACGACAC 501 GATTTATGTT TTGACCCCGC ACGGCAAAGT CCTCTCCCTG CCCACGGGCG 551 CGACCCCCAT CGACTTCGCC TACGCCCTGC ACAGCAGCAT CGGCGACCGT 601 TGCCGCGGTG CGAAAGTCGA AGGGCAGATT GTGCCGCTGT CCACCCCGCT 651 CGAAAACGGA CAGCGCGTCG AAATCATTAC CGCCAAAGAA GGGCATCCTT 701 CCGTCAACTG GCTTTACGAA GGCTGGGTCA AATCCAACAA GGCAATCGGC 751 AAAATCCGCG CCTACATCCG CCAGCAAAAC GCCGACACCG TGCGCGAAGA 801 AGGCCGCGTC CAACTCGACA AACAGCTTGC CAAACTCACG CCCAAACCCA 851 ACCTGCAAGA GCTTGCCGAA AATCTCGGCT ACAAAAAGCC AGAAGACCTC 901 TACACCGCCG TCGGACAAGG CGAAATTTCC AACCGCGCCA TCCAAAAAGC 951 CTGCGGCACg CTGAACGAAC CGCCGCCCGT ACCCGTCAGC GAAACCACCA 1001 TCGTCAAACA GTCCAAAATC AAAAAAGGCG GCAAAAACGG CGTGCTCATC 1051 GACGGCGAAG ACGGTCTGAT GACCACGCTT GCCAAATGCT GCAAACCCGC 1101 GCCGCCCGAC GATATTATCG GCTTCGTTAC CCGCGAGCGC GgCATTTCAG 1151 TGCACCGCAA AwyyTkCyCG TCTTTCCAAC ACCTCGCCGA ACACGCGCCC 1201 GAwAAAGTGC TGGACGCAAG CTGGGCGGCA TTGCAGGAAG GACAAGTATT 1251 CGCCGTCGAT ATCGAAATCC GCGCCCAAGA CCGCTCCGGG CTTTTGCGCG 1301 ACGTATCCGA CGCGCTCGCC CGCCACAAAC TCAACGTTAC CGCCGTGCAA 1351 ACCCAGTCCC GCGACTTGGA AGCCAGCATG AGGTTCACGC TCGAAGTCAA 1401 ACAAGTCAAC GACCTCCCGC GCGTCCTCGC CAGCCTCGGC GACGTCAAAG 1451 GCGTATTGAG CGTTACCCGG CTTTAA This corresponds to the amino acid sequence <SEQ ID 414; ORF 117>: mll7 .pep (partial ) 1....VKLKKYNVHF EVAGRPKHIY SIYKKMVKKK LSFDGLFDIR AVRILVDTVP 51 ECYTTLGIVH SLWQPIPGEF DDYIANPKGN GYKSLHTVIV GPEDKGVEVQ 101 IRTFDMHQFN EFGVAAHWRY KEGGKGDSAY EQKIAWLRQL LDWRENMAES 151 GKEDLAAAFK TELFNDTIYV LTPHGKVLSL PTGATPIDFA YALHSSIGDR 201 CRGAKVEGQI VPLSTPLENG QRVEIITAKE GHPSVNWLYE GWVKSNKAIG 251 KIRAYIRQQN ADTVREEGRV QLDKQLAKLT PKPNLQELAE NLGYKKPEDL 301 YTAVGQGEIS NRAIQKACGT LNEPPPVPVS ETTIVKQSKI KKGGKNGVLI 351 DGEDGLMTTL AKCCKPAPPD DIIGFVTRER GISVHRKXXX SFQHLAEHAP 401 XKVLDASWAA LQEGQVFAVD IEIRAQDRSG LLRDVSDALA RHKLNVTAVQ 451 TQSRDLEASM RFTLEVKQVN DLPRVLASLG DVKGVLSVTR L* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N. gonorrhoeae ORF 117 shows 97.6% identity over a 490 aa overlap with a predicted ORF (ORF 117.ng) from N. gonorrhoeae: mll7/gll7 10 20 30 mll7.pep VKLKKYΝVHFEVAGRPKHIYSIYKKMVKKKL = ! 111 I = 11 i 1111111 i 111111 ] F I [ I ! gll7 EKYREIALLLDEKRTERLEYIEΝFLDILRTELKKYΝIHFEVAGRPKHIYSIYKKMVKKKL 150 160 170 180 190 200 40 50 60 70 80 90 ml17. pep SFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIAΝPKGΝGYKSLHTVIVG gll7 SFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIAΝPKGΝGYKSLHTVIVG 210 220 230 240 250 260 100 110 120 130 140 150 mll7.pep PEDKGVEVQIRTFDMHQFΝEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREΝMAESG gll7 PEDKGVEVQIRTFDMHQFΝEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREΝMAESG 270 280 290 300 310 320 160 170 180 190 200 210 mll7.pep KEDLAAAFKTELFΝDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGAKVEGQIV gll7 KEDLAAAFKTELFΝDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGAKVEGQIV 330 340 350 360 370 380 220 230 240 250 260 270 mll7.pep PLSTPLEΝGQRVEIITAKEGHPSVΝWLYEGWVKSΝKAIGKIRAYIRQQΝADTVREEGRVQ gll7 PLSTPLEΝGQRVEIITAKEGHPSVΝWLYEGWVKSGKAIGKIRAYIRQQΝADTVREEGRVQ 390 400 410 420 430 440 280 290 300 310 320 330 ml17. pep LDKQLAKLTPKPΝLQELAEΝLGYKKPEDLYTAVGQGEISΝRAIQKACGTLΝEPPPVPVSE gll7 LDKQLAKLTPKPΝLQELAEΝLGYKKPEDLYTAVGQGEISΝRAIQKACGTLΝEPPPVPVSA 450 460 470 480 490 500 340 350 360 370 380 390 mll7.pep TTIVKQSKIKKGGKΝGVLIDGEDGLMTTLAKCCKPAPPDDIIGFVTRERGISVHRKXXXS 11111 II 1111111 : I gll7 TTIVK Q SKIKKGGKTGVLIDGEDGLMTTLAKCCKPAPPDDIAGFVTRERGISVHRKTCPS 510 520 530 540 550 560 400 410 420 430 440 450 mll7.pep F Q HLAEHAPXKVLDASWAAL Q EG Q VFAVDIEIRAQDRSGLLRDVSDALARHKLΝVTAVQT gll7 FRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVSDALARHKLNVTAVQT 570 580 590 600 610 620 460 470 480 490 ml17. pep QSRDLEASMRFTLEVKQVNDLPRVLASLGDVKGVLSVTRLX l l l l l l l l l l l l l l l l l l l l l l l l l l l l l l l l l l l l l l l gl 17 QSRDLEASMRFTLEVKQVNDLPRVLAGLGDVKGVLSVTRLX 630 640 650 660 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 415>: all7.seq 1 ATGGTTCATG AACTCGACCT GCTCCCCGAT GCCGTCGCCG CCACCCTGCT 51 TGCCGACATC GGACGCTACG TCCCCGACTG GAACCTATTG GTTTCCGAAC 101 GCTGCAACAG TACCGTCGCC GAGCTGGTCA AAGGTGTGGA CGAAGTGCAG 151 AAACTCACCC ACTTCGCCCG GGTGGACAGC CTCGCCACGC CGGAAGAACG 201 CGCCCAGCAG GCAGAAACTA TGCGGAAAAT GCTGCTGGCG ATGGTTACCG 251 ACATCCGCGT CGTGTTAATC AAACTGGCGA TGCGTACGCG CACCCTGCAA 301 TTTTTAAGCA ACGCCCCCGA CAGCCCCGAA AAACGCGCCG TCGCCAAAGA 351 AACCCTCGAC ATCTTCGCCC CGCTCGCCAA CCGTTTGGGC GTGTGGCAGC 401 TCAAATGGCA GCTCGAAGAT TTGGGCTTCC GCCATCAAGA ACCCGAAAAA 451 TACCGCGAAA TCGCCCTGCT TTTGGACGAA AAACGCACCG AACGCCTCGA 501 ATACATCGAA AACTTCCTTA ATATCCTGCG TACGGAACTC AAAAAATACA 551 ATATCCACTT TGAAGTCGCC GGCCGTCCGA AACACATCTA CTCCATTTAC 601 AAAAAAATGG TGAAGAAAAA ACTCAGCTTC GACGGGTTGT TCGACATCCG 651 CGCCGTGCGG ATTCTGGTTG ATACCGTCCC CGAGTGTTAC ACCACACTGG 701 GCATTGTCCA CAGCCTCTGG CAGCCCATTC CCGGCGAGTT CGACGACTAC 751 ATCGCCAACC CGAAAGGCAA CGGCTATAAA AGTTTGCACA CCGTCATCGT 801 CGGCCCGGAA GACAAAGGCG TGGAAGTGCA AATCCGCACC TTCGATATGC 851 ACCAATTCAA CGAATTCGGT GTCGCCGCGC ACTGGCGTTA CAAAGAGGGC 901 GGCAAAGGCG ATTCCGCCTA CGAACAAAAA ATCGCCTGGT TACGCCAACT 951 TTTGGACTGG CGCGAAAACA TGGCGGAAAG CGGCAAGGAA GACCTCGCCG 1001 CCGCCTTCAA AACCGAGCTT TTCAACGACA CGATTTATGT TTTGACCCCG 1051 CACGGCAAAG TCCTCTCCCT GCCCACAGGC GCGACCCCCA TCGACTTCGC 1101 CTACGCCCTG CACAGCAGCA TCGGCGACCG TTGCCGCGGT GCGAAAGTCG 1151 AAGGGCAGAT TGTGCCGCTG TCCACCCCGC TCGAAAACGG ACAGCGTGTC 1201 GAAATCATTA CCGCCAAAGA AGGGCATCCT TCCGTCAACT GGCTTTACGA 1251 AGGCTGGGTC AAATCCAACA AGGCAATCGG CAAAATCCGC GCCTACATCC 1301 GCCAGCAAAA CGCCGACACC GTGCGCGAAG AAGGCCGCGT CCAACTCGAC 1351 AAACAGCTTG CCAAACTCAC GCCCAAACCC AACCTGCAAG AGCTTGCCGA 1401 AAATCTCGGC TACAAAAAGC CAGAAGACCT CTACACCGCC GTCGGACAAG 1451 GCGAAATTTC CAACCGCGCC ATCCAAAAAG CCTGCGGCAC GCTGAACGAA 1501 CCGCCGCCCG TACCCGTCAG CGAAACCACC ATCGTCAAAC AGTCCAAAAT 1551 CAAAAAAGGC GGCAAAAACG GCGTGCTCAT CGACGGCGAA GACGGTCTGA 1601 TGACCACGCT TGCCAAATGC TGCAAACCCG CGCCGCCCGA CGACATTGTC 1651 GGCTTCGTTA CCCGCGATCG CGGCATTTCG GTACACCGCA AAACCTGCCC 1701 CTCTTTCCGA CACCTCGCCG AACACGCGCC CGAAAAAGTA CTGGACGCAA 1751 GTTGGGCGGC GTTGCAGGAA GGACAAGTGT TCGCCGTCGA TATCGAAATC 1801 CGCGCCCAAG ACCGCTCCGG GCTTTTGCGC GACGTATCCG ACGCGCTCGC 1851 CCGCCACAAA CTCAACGTTA CCGCCGTGCA AACCCAGTCC CGCGACTTGG 1901 AAGCCAGCAT GAGGTTCACG CTCGAAGTCA AACAAGTTAC CGACCTCCCA 1951 CGCGTCCTCG CCAGCCTCGG CGACGTCAAA GGCGTATTGA GCGTTACCCG 2001 GCTTTAA This corresponds to the amino acid sequence <SEQ ID 416; ORF 117. a>: all7.pep 1 MVHELDLLPD AVAATLLADI GRYVPDWΝLL VSERCΝSTVA ELVKGVDEVQ 51 KLTHFARVDS LATPEERAQQ AETMRKMLLA MVTDIRWLI KLAMRTRTLQ 101 FLSΝAPDSPE KRAVAKETLD IFAPLAΝRLG VWQLKWQLED LGFRHQEPEK 151 YREIALLLDE KRTERLEYIE ΝFLΝILRTEL KKYΝIHFEVA GRPKHIYSIY 201 KKMVKKKLSF DGLFDIRAVR ILVDTVPECY TTLGIVHSLW QPIPGEFDDY 251 IAΝPKGΝGYK SLHTVIVGPE DKGVEVQIRT FDMHQFΝEFG VAAHWRYKEG 301 GKGDSAYEQK IAWLRQLLDW REΝMAESGKE DLAAAFKTEL FΝDTIYVLTP 351 HGKVLSLPTG ATPIDFAYAL HSSIGDRCRG AKVEGQIVPL STPLEΝGQRV 401 EIITAKEGHP SVNWLYEGWV KSNKAIGKIR AYIRQQNADT VREEGRVQLD 451 KQLAKLTPKP NLQELAENLG YKKPEDLYTA VGQGEISNRA IQKACGTLNE 501 PPPVPVSETT IVKQSKIKKG GKNGVLIDGE DGLMTTLAKC CKPAPPDDIV 551 GFVTRDRGIS VHRKTCPSFR HLAEHAPEKV LDASWAALQE GQVFAVDIEI 601 RAQDRSGLLR DVSDALARHK LNVTAVQTQS RDLEASMRFT LEVKQVTDLP 651 RVLASLGDVK GVLSVTRL* mll7/all7 98.0% identity in 490 aa overlap 10 20 30 mll .pep VKLKKYNVHFEVAGRPKHIYSIYKKMVKKKL : I I I I I : I I I I I I I I I I I I I I I I I I I I I I I all7 EKYREIALLLDEKRTERLEYIENFLNILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKKL 150 160 170 180 190 200 40 50 60 70 80 90 mll7.pep SFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTVIVG I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I all7 SFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTVIVG 210 220 230 240 250 260 100 110 120 130 140 150 mll7. pep PEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWRENMAESG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I all7 PEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWRENMAESG 270 280 290 300 310 320 160 170 180 190 200 210 mll7. pep KEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGAKVEGQIV I I I I I I I I I I I I I I I I II I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I II I I I I I I I I all7 KEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGAKVEGQIV 330 340 350 360 370 380 220 230 240 250 260 270 ml17. pep PLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSNKAIGKIRAYIRQQNADTVREEGRVQ I I I I I I I I II I I I I I I I II II I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I all7 PLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSNKAIGKIRAYIRQQNADTVREEGRVQ 390 400 410 420 430 440 280 290 300 310 320 330 mll7. pep LDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGTLNEPPPVPVSE I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I all7 LDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGTLNEPPPVPVSE 450 460 470 480 490 500 340 350 360 370 380 390 mll7.pep TTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIIGFVTRERGISVHRKXXXS I II I I I I I I II I I I I I I I I II I I I I II I I II I II I I I I I I I : I I I I I : I I I I I I I I : I all7 TTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTRDRGISVHRKTCPS 510 520 530 540 550 560 400 410 420 430 440 450 mll7. pep FQHLAEHAPXKVLDASWAALQEGQVFAVDIEIRAQDRΞGLLRDVSDALARHKLNVTAVQT l l l l l l l I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I all7 FRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVSDALARHKLNVTAVQT 570 580 590 600 610 620 460 470 480 490 mll7.pep QSRDLEASMRFTLEVKQVNDLPRVLASLGDVKGVLSVTRLX I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I all7 QSRDLEASMRFTLEVKQVTDLPRVLASLGDVKGVLSVTRLX 630 640 650 660 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 417>: gll7-l.seq 1 ATGACCGCCA TCAGCCCGAT TCAAGACACG CAAAGCGCGA CCCTGCAAGA 51 ATTGCGCGAA TGGTTCGACA GCTACTGCGC CGCTCTGCCG GACAACGATA 101 AAAACCTCAT CGGTACCGCA TGGTCGCTGG CGCAGGAACA TTATCCTGCC 151 GATGCCGCCA CGCCGTATGG CGAGCCGCTG CCCGACCACT TCCTCGGCGC 201 GGCGCAAATG GTCGACGAAC TCGACCTGCT GCCCGATGCC GTCGCCGCCA 251 CCCTGCTTGC CGACATCGGA CGCTACGTCC CCGATTGGAA CCTATTGGTT 301 TCCGAGCGCT GCAACAGCAC CGTCGCCGAG CTGGTCAAAG GTGTGGACGA 351 AGTGCAGAAA CTTACCCACT TCGCCCGGGT GGACAGCCTC GCCACGCCGG 401 AAGAACGCGC ACAGCAAGCG GAAACCATGC GGAAAATGCT GCTGGCGATG 451 GTTACCGACA TCCGCGTCGT ATTAATCAAA CTGGCGATGC GTACGCGCAC 501 CCTGCAATTT TTAAGCAACG CCCCCGACAG CCCTGAAAAA CGCGCCGTCG 551 CCAAAGAAAC CCTCGACATC TTCGCCCCGC TCGCCAACCG CTTGGGCGTG 601 TGGCAGCTCA AATGGCAGCT CGAAGATTTG GGCTTCCGCC ATCAAGAACC 651 CGAAAAATAC CGCGAAATCG CCCTGCTTTT GGACGAAAAA CGCACCGAAC 701 GCCTCGAATA CATCGAAAAC TTCCTCGATA TCCTGCGTAC GGAACTCAAA 751 AAATACAATA TCCACTTTGA AGTCGCCGGC CGTCCGAAAC ACATCTACTC 801 CATTTACAAA AAAATGGTGA AGAAAAAACT CAGCTTCGAC GGCCTGTTCG 851 ACATCCGCGC CGTGCGGATT CTGGTCGATA CCGTCCCCGA GTGTTACACC 901 ACGCTGGGCA TCGTCCACAG CCTCTGGCAG CCCATTCCCG GCGagttCGA 951 cgactACATC GCCAACCCCA AAGgcaACGg ttATAAAAGt TTGCACACCG 1001 TCATCGTcgg cccGGAagaa aaaggtgtgg aagtgcAAAT CCGCACCTTC 1051 GATATGCacc AATTCaaCga ATTCGGTGTC GCCGCCCACT GGCGTTACAA 1101 AGAAGGCGGC AAAGGCGATT CCGCCTACGA ACAAAAAATC GCCTGGTTGC 1151 GCCAACTCTT GGACTGGCGC GAAAATATGG CGGAAAGCGG CAAGGAAGAC 1201 CTCGCCGCCG CCTTCAAAAC CGAGCTTTTC AACGACACGA TTTATGTTTT 1251 GACCCCGCAC GGCAAAGTCC TCTCTCTGCC AACGGGCGCA ACCCCCATCG 1301 ACTTCGCCTA CGCCCTGCAC AGCAGCATCG GCGACCGCTG CCGGGGCGCG 1351 AAAGTCGAAG GGCAGATTGT GCCGCTGTCC ACCCCGCTCG AAAACGGACA 1401 GCGCGTCGAA ATCATTACCG CCAAAGAAGG GCATCCTTCC GTCAACTGGC 1451 TTTACGAAGG CTGGGTCAAA TCCGGCAAGG CCATCGGCAA AATCCGCGCC 1501 TACATCCGCC AGCAAAACGC CGACACCGTG CGCGAAGAAG GCCGTGTCCA 1551 ACTCGACAAG CAGCTTGCCA AACTCACGCC CAAACCCAAC CTGCAAGAGC 1601 TTGCCGAAAA TCTCGGCTAC AAAAAGCCAG AAGACCTCTA CACCGCCGTC 1651 GGACAAGGCG AAATTTCCAA CCGCGCCATC CAAAAAGCCT GCGGCACGCT 1701 GAACGAACCG CCGCCCGTGC CCGTCAGCGC AACCACCATC GTCAAACAGT 1751 CCAAAATCAA AAAAGGTGGC AAAACCGGCG TGCTCATCGA CGGCGAAGAC 1801 GGCTTGATGA CCACGCTTGC CAAATGCTGC AAACCCGCGC CGCCCGACGA 1851 TATTGCCGGC TTCGTTACCC GCGAGCGCGG CATTTCCGTC CACCGCAAAA 1901 CCTGCCCCTC TTTCCGACAC CTTGCCGAAC ACGCGCCCGA AAAAGTACTG 1951 GACGCAAGTT GGGCGGCGTT GCAGGAAGGG CAAGTGTTCG CCGTCGATAT 2001 CGAAATCCGC GCCCAAGACC GCTCCGGGCT TTTGCGCGAC GTATCCGACG 2051 CGCTCGCCCG CCACAAACTC AACGTTACCG CCGTGCAAAC CCAGTCCCGC 2101 GACTTGGAAG CCAGCATGAG GTTCACGCTC GAAGTCAAAC AAGTCAACGA 2151 CCTCCCGCGC GTCCTCGCCG GCCTCGGCGA TGTCAAAGGC GTATTGAGCG 2201 TTACCCGGCT TTAA This corresponds to the amino acid sequence <SEQ ID 418; ORF 117-l.ng>: gll7-l.pep 1 MTAISPIQDT QSATLQELRE WFDSYCAALP DΝDKΝLIGTA SLAQEHYPA 51 DAATPYGEPL PDHFLGAAQM VDELDLLPDA VAATLLADIG RYVPDWΝLLV 101 SERCΝΞTVAE LVKGVDEVQK LTHFARVDSL ATPEERAQQA ETMRKMLLAM 151 VTDIRVVLIK LAMRTRTLQF LΞΝAPDSPEK RAVAKETLDI FAPLAΝRLGV 201 QLKWQLEDL GFRHQEPEKY REIALLLDEK RTERLEYIEΝ FLDILRTELK 251 KYΝIHFEVAG RPKHIYSIYK KMVKKKLSFD GLFDIRAVRI LVDTVPECYT 301 TLGIVHΞL Q PIPGEFDDYI AΝPKGΝGYKS LHTVIVGPEE KGVEVQIRTF 351 DMHQFΝEFGV AAH RYKEGG KGDSAYEQKI AWLRQLLDWR EΝMAESGKED 401 LAAAFKTELF ΝDTIYVLTPH GKVLΞLPTGA TPIDFAYALH SSIGDRCRGA 451 KVEGQIVPLS TPLEΝGQRVE IITAKEGHPS VΝ LYEGWVK SGKAIGKIRA 501 YIRQQΝADTV REEGRVQLDK QLAKLTPKPΝ LQELAEΝLGY KKPEDLYTAV 551 GQGEISΝRAI QKACGTLΝEP PPVPVSATTI VKQSKIKKGG KTGVLIDGED 601 GLMTTLAKCC KPAPPDDIAG FVTRERGIΞV HRKTCPSFRH LAEHAPEKVL 651 DASWAALQEG QVFAVDIEIR AQDRΞGLLRD VSDALARHKL ΝVTAVQTQSR 701 DLEASMRFTL EVKQVΝDLPR VLAGLGDVKG VLSVTRL* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 419>: mll7-l.seq 1 ATGACCGCCA TCAGCCCGAT TCAAGACACG CAAAGCGCGA CTCTGCAAGA 51 ATTGCGCGAA TGGTTCGACA GCTACTGCGC CGCTCTGCCG GACAACGATA 101 AAAACCTCAT CGGTACCGCA TGGTTGCTGG CGCAGGAACA TTACCCCGCC 151 GATGCCGCCA CGCCGTATGG CGAGCCGCTG CCCGACCACT TCCTCGGCGC 201 GGCGCAAATG GTTCATGAAC TCGACCTGCT CCCCGATGCC GTCGCCGCCA 251 CCCTGCTTGC CGACATCGGA CGCTACGTCC CCGACTGGAA CCTATTGGTT 301 TCCGAACGCT GCAACAGTAC CGTCGCCGAG CTGGTCAAAG GTGTGGACGA 351 AGTGCAGAAA CTCACCCACT TCGCCCGGGT GGACAGCCTC GCCACGCCGG 401 AAGAACGCGC CCAGCAGGCA GAAACTATGC GGAAAATGCT GCTGGCGATG 451 GTTACCGACA TCCGCGTCGT GTTAATCAAA CTGGCGATGC GTACGCGCAC 501 CCTGCAATTT TTAAGCAACG CCCCCGACAG CCCCGAAAAA CGCGCCGTCG 551 CCAAAGAAAC CCTCGACATC TTCGCCCCGC TCGCCAACCG TTTGGGCGTG 601 TGGCAGCTCA AATGGCAGCT CGAAGATTTG GGCTTCCGCC ATCAAAAGCC 651 CGAAAAATAC CGCGAAATCG CGCTGCTTTT GGACGAAAAA CGCACCGAAC 701 GCCTCGAATA CATCGAAAAC TTCCTCAACA TCCTGCGCGG TGAACTCAAG 751 AAATACAATG TCCATTTCGA AGTCGCCGGC CGCCCGAAAC ACATCTACTC 801 CATTTACAAA AAAATGGTGA AGAAAAAACT CAGCTTCGAC GGCCTCTTTG 851 ACATCCGCGC CGTGCGAATT CTGGTTGATA CCGTCCCCGA GTGTTACACC 901 ACGCTGGGTA TCGTCCACAG CCTCTGGCAG CCCATTCCCG GCGAGTTCGA 951 CGACTACATC GCCAATCCCA AAGGCAACGG CTATAAAAGT TTGCACACCG 1001 TCATCGTCGG CCCGGAAGAC AAAGGCGTGG AAGTACAAAT CCGCACCTTC 1051 GATATGCACC AATTCAACGA ATTCGGTGTC GCCGCCCACT GGCGTTACAA 1101 AGAGGGCGGC AAGGGCGATT CCGCCTACGA ACAGAAAATC GCCTGGTTGC 1151 GCCAACTCTT GGACTGGCGC GAAAACATGG CGGAAAGCGG CAAGGAAGAC 1201 CTCGCCGCCG CCTTCAAAAC CGAGCTTTTC AACGACACGA TTTATGTTTT 1251 GACCCCGCAC GGCAAAGTCC TCTCCCTGCC CACGGGCGCG ACCCCCATCG 1301 ACTTCGCCTA CGCCCTGCAC AGCAGCATCG GCGACCGTTG CCGCGGTGCG 1351 AAAGTCGAAG GGCAGATTGT GCCGCTGTCC ACCCCGCTCG AAAACGGACA 1401 GCGCGTCGAA ATCATTACCG CCAAAGAAGG GCATCCTTCC GTCAACTGGC 1451 TTTACGAAGG CTGGGTCAAA TCCAACAAGG CAATCGGCAA AATCCGCGCC 1501 TACATCCGCC AGCAAAACGC CGACACCGTG CGCGAAGAAG GCCGCGTCCA 1551 ACTCGACAAA CAGCTTGCCA AACTCACGCC CAAACCCAAC CTGCAAGAGC 1601 TTGCCGAAAA TCTCGGCTAC AAAAAGCCAG AAGACCTCTA CACCGCCGTC 1651 GGACAAGGCG AAATTTCCAA CCGCGCCATC CAAAAAGCCT GCGGCACGCT 1701 GAACGAACCG CCGCCCGTAC CCGTCAGCGA AACCACCATC GTCAAACAGT 1751 CCAAAATCAA AAAAGGCGGC AAAAACGGCG TGCTCATCGA CGGCGAAGAC 1801 GGTCTGATGA CCACGCTTGC CAAATGCTGC AAACCCGCGC CGCCCGACGA 1851 TATTATCGGC TTCGTTACCC GCGAGCGCGG CATTTCAGTG CACCGCAAAA 1901 CCTGCCCGTC TTTCCAACAC CTCGCCGAAC ACGCGCCCGA AAAAGTGCTG 1951 GACGCAAGCT GGGCGGCATT GCAGGAAGGA CAAGTATTCG CCGTCGATAT 2001 CGAAATCCGC GCCCAAGACC GCTCCGGGCT TTTGCGCGAC GTATCCGACG 2051 CGCTCGCCCG CCACAAACTC AACGTTACCG CCGTGCAAAC CCAGTCCCGC 2101 GACTTGGAAG CCAGCATGAG GTTCACGCTC GAAGTCAAAC AAGTCAACGA 2151 CCTCCCGCGC GTCCTCGCCA GCCTCGGCGA CGTCAAAGGC GTATTGAGCG 2201 TTACCCGGCT TTAA This corresponds to the amino acid sequence <SEQ ID 420; ORF 117-1>: mll7-l .pep 1 MTAISPIQDT QSATLQELRE FDSYCAALP DNDKNLIGTA WLLAQEHYPA 51 DAATPYGEPL PDHFLGAAQM VHELDLLPDA VAATLLADIG RYVPDWNLLV 101 ΞERCNSTVAE LVKGVDEVQK LTHFARVDSL ATPEERAQQA ETMRKMLLAM 151 VTDIRVVLIK LAMRTRTLQF LSNAPDSPEK RAVAKETLDI FAPLANRLGV 201 WQLK QLEDL GFRHQKPEKY REIALLLDEK RTERLEYIEN FLNILRGELK 251 KYNVHFEVAG RPKHIYSIYK KMVKKKLSFD GLFDIRAVRI LVDTVPECYT 301 TLGIVHSL Q PIPGEFDDYI ANPKGNGYKS LHTVIVGPED KGVEVQIRTF 351 DMHQFNEFGV AAH RYKEGG KGDSAYEQKI AWLRQLLDWR ENMAESGKED 401 LAAAFKTELF NDTIYVLTPH GKVLSLPTGA TPIDFAYALH SSIGDRCRGA 451 KVEGQIVPLS TPLENGQRVE IITAKEGHPS VN LYEGWVK SNKAIGKIRA 501 YIRQQNADTV REEGRVQLDK QLAKLTPKPN LQELAENLGY KKPEDLYTAV 551 GQGEISNRAI QKACGTLNEP PPVPVSETTI VKQSKIKKGG KNGVLIDGED 601 GLMTTLAKCC KPAPPDDIIG FVTRERGISV HRKTCPSFQH LAEHAPEKVL 651 DASWAALQEG QVFAVDIEIR AQDRSGLLRD VSDALARHKL NVTAVQTQSR 701 DLEASMRFTL EVKQVNDLPR VLASLGDVKG VLSVTRL* mll7-l/gll7-l 98.2% identity in 737 aa overlap 10 20 30 40 50 60 ml17-1. pep MTAISPIQDTQSATLQELRE FDSYCAALPDNDKNLIGTA LLAQEHYPADAATPYGEPL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gll7-l MTAISPIQDTQSATLQELREWFDSYCAALPDNDKNLIGTAWSLAQEHYPADAATPYGEPL 10 20 30 40 50 60 70 80 90 100 110 120 ml17-1. pep PDHFLGAAQMVHELDLLPDAVAATLLADIGRYVPD NLLVSERCNSTVAELVKGVDEVQK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g117-1 PDHFLGAAQMVDELDLLPDAVAATLLADIGRYVPD NLLVSERCNSTVAELVKGVDEVQK 70 80 90 100 110 120 130 140 150 160 170 180 mll7-l.pep LTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRWLIKLAMRTRTLQFLSNAPDSPEK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gll7-l LTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRWLIKLAMRTRTLQFLSNAPDSPEK 130 140 150 160 170 180 190 200 210 220 230 240 ml17-1. pep RAVAKETLDIFAPLANRLGV QLK QLEDLGFRHQKPEKYREIALLLDEKRTERLEYIEN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I gll7-l RAVAKETLDIFAPLANRLGV QLK QLEDLGFRHQEPEKYREIALLLDEKRTERLEYIEN 190 200 210 220 230 240 250 260 270 280 290 300 ml17-1. pep FLNILRGELKKYNVHFEVAGRPKHIYSIYKKMVKKKLSFDGLFDIRAVRILVDTVPECYT 11:111 I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gll7-l FLDILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKKLSFDGLFDIRAVRILVDTVPECYT 250 260 270 280 290 300 310 320 330 340 350 360 mll7-l.pep TLGIVHSL QPIPGEFDDYIANPKGNGYKSLHTVIVGPEDKGVEVQIRTFDMHQFNEFGV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I gll7-l TLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTVIVGPEEKGVEVQIRTFDMHQFNEFGV 310 320 330 340 350 360 370 380 390 400 410 420 ml17-1. pep AAHWRYKEGGKGDSAYEQKIA LRQLLD RENMAESGKEDLAAAFKTELFNDTIYVLTPH I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I II I I I I I I I gll7-l AAHWRYKEGGKGDSAYEQKIA LRQLLDWRENMAESGKEDLAAAFKTELFNDTIYVLTPH 370 380 390 400 410 420 430 440 450 460 470 480 mll7-l.pep GKVLSLPTGATPIDFAYALHSSIGDRCRGAKVEGQIVPLSTPLENGQRVEIITAKEGHPS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gll7-l GKVLSLPTGATPIDFAYALHSSIGDRCRGAKVEGQIVPLSTPLENGQRVEIITAKEGHPS 430 440 450 460 470 480 490 500 510 520 530 540 mll7-l . pep VNWLYEG VKSNKAIGKIRAYIRQQNADTVREEGRVQLDKQLAKLTPKPNLQELAENLGY I I I I II I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gll7-l VN LYEGWVKSGKAIGKIRAYIRQQNADTVREEGRVQLDKQLAKLTPKPNLQELAENLGY 490 500 510 520 530 540 550 560 570 580 590 600 mll7-l.pep KKPEDLYTAVGQGEISNRAIQKACGTLNEPPPVPVΞETTIVKQΞKIKKGGKNGVLIDGED I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I :| I I I I I I I gll7-l KKPEDLYTAVGQGEISNRAIQKACGTLNEPPPVPVSATTIVKQSKIKKGGKTGVLIDGED 550 560 570 580 590 600 610 620 630 640 650 660 mll7-l.pep GLMTTLAKCCKPAPPDDIIGFVTRERGISVHRKTCPSFQHLAEHAPEKVLDASWAALQEG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I gll7-l GLMTTLAKCCKPAPPDDIAGFVTRERGISVHRKTCPSFRHLAEHAPEKVLDAS AALQEG 610 620 630 640 650 660 670 680 690 700 710 720 mll7-l . pep QVFAVDIEIRAQDRSGLLRDVSDALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gll7-l QVFAVDIEIRAQDRSGLLRDVSDALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPR 670 680 690 700 710 720 730 ml17-1. pep VLASLGDVKGVLSVTRLX I I I : I I I I I I I I I I I I I I gll7-l VLAGLGDVKGVLSVTRLX 730 mll7-l/RelA sp|P55133|RELA__VIBSS GTP PYROPHOSPHOKINASE (ATP:GTP 3 ' -PYROPHOSPHOTRANSFERASE) (PPGPP SYNTHETASE I) >gι 1537617 (013769) ppGpp synthetase I [Vibrio sp.] Length = 744 Score = 536 bits (1366), Expect = e-151 Identities = 288/685 (42%), Positives = 432/685 (63%), Gaps = 31/685 (4%) Query: 74 LDLLPDAVAATLLADI GRYVPDWNLLVSERCNSTVAELVKGVDEVQKLTHFARVDSL 130 L + D + A LL + G Y D + E + T+ LV+GV+++ ++ ++ S Sb j ct: 68 LSMDADTLIAALLYPLVEGGCYSTD ALKEEYSGTILHLVQGVEQMCAIS QLKST 121 Query: 131 ATPEERAQQAETMRKMLLAMVTDIRVVLIKLAMRTRTLQFLSNAPDSPEKRAVAKETLDI 190 A +A Q + +R+MLL+MV D R V+IKLA R L+ + + PD +RA A+E +1 Sb j ct: 122 AEETAQAAQVDNIRRMLLSMVDDFRCWIKLAERICNLREVKDQPDEV-RRAAAQECANI 180 Query: 191 FAPLANRLGV QLKWQLEDLGFRHQKPEKYREIALLLDEKRTERLEYIENFLNILRGELK 250 +APLANRLG+ QLK ++ED FR+Q P+ Y++IA L E+R +R +YI +F++ L +K Sb j ct: 181 YAPLANRLGIGQLKWEIEDYAFRYQHPDTYKQIAKQLSERRIDREDYITHFVDDLSDAMK 240 Query: 251 KYNVHFEVAGRPKHIYSIYKKMVKKKLSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQ 310 N+ EV GRPKHIYSI++KM KK L FD LFD+RAVRI+ + + +CY LG+VH+ ++ Sb j ct: 241 ASNIRAEVQGRPKHIYSIWRKMQKKSLEFDELFDVRAVRIVAEELQDCYAALGWHTKYR 300 Query: 311 PIPGEFDDYIANPKGNGYKSLHTVIVGPEDKGVEVQIRTFDMHQFNEFGVAAH RYKEG- 369 +P EFDDY+ANPK NGY+S+HTV++GPE K +E+QIRT MH+ +E GVAAH +YKEG Sb j ct: 301 HLPKEFDDYVANPKPNGYQSIHTWLGPEGKTIEIQIRTKQMHEESELGVAAH KYKEGT 360 Query: 370 —GKGDSAYEQKIAWLRQLLDWRENMAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLP 427 G SAY++KI WLR+LL W+E M++SG ++ ++++F+D +Y TP G V+ LP Sb j ct: 361 ASGGAQSAYDEKIN LRKLLA QEEMSDSG—EMLDELRSQVFDDRVYAFTPKGDVVDLP 418 Query: 428 TGATPIDFAYALHSSIGDRCRGAKVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYE- 486 + ATP+DFAY +HS +G RC GAKVEG+IVP + L+ G +VEIIT KE +PS + L Sbjct: 419 SNATPLDFAYHIHSEVGHRCIGAKVEGRIVPFTYHLQMGDQVEIITQKEPNPSRDWLNPN 478 Query: 487 -GWVKSNKAIGKIRAYIRQQNADTVREEGRVQLDKQLAK —TPKPNLQELAENLGYKKP 543 G+V S++A K+ A+ R+Q+ D G+ L+ +L K+ T K A+ K P Sb j ct: 479 LGFVTSSRARAKVHA FRKQDRDKNIIAGKEILEAELVKIHATLKDAQYYAAKRFNVKSP 538 Query: 544 EDLYTAVGQGEIS-NRAIQKACGTLNEPPPVPVSETTIVKQSKI KKGGKNGV 594 E+LY +G G++ N+ I +N+P + + K S+ KK ++ V Sb j ct: 539 EELYAGIGSGDLRINQVINHINALVNKPTAEEEDQQLLEKLΞEASNKQATΞHKKPQRDAV 598 Query: 595 LIDGEDGLMTTLAKCCKPAPPDDIIGFVTRERGISVHRKTCPSFQHLAEHAPEKVLDASW 654 +++G D LMT LA+CC+P P DDI GFVT+ RGIΞVHR C + L HAPE+++D W Sb j ct: 599 WEGVDNLMTHLARCCQPIPGDDIQGFVTQGRGISVHRMDCEQLEELRHHAPERIIDTV 658 Query: 655 AALQEGQVFAVDIEIRAQDRSGLLRDVSDALARHKLNVTAVQTQ—SRDLEASMRFTLEV 712 G + + + + A +R+GLL+++++ L K+ V ++++ + + M F LE+ Sbjct: 659 GGGFVGN-YTITVRVTAΞERNGLLKELTNTLMNEKVKVAGMKSRVDYKKQMSIMDFELEL 717 Query: 713 KQVNDLPRVLASLGDVKGVLSVTRL 737 + L RVL + VK V RL Sbjct: 718 TDLEVLGRVLKRIEQVKDVAEAKRL 742 The following partial DNA sequence was identified in N meningitidis <SEQ ID 421>: all7-l.seq 1 ATGACCGCCA TCAGCCCGAT TCAAGACACG CAAAGCGCGA CTCTGCAAGA 51 ATTGCGCGAA TGGTTCGACA GCTACTGCAC CGCGCTGCCG AACAACGATA 101 AAAAACTTGT CTTAGCCGCC CGTTCGCTGG CGGAAGCACA TTACCCCGCC 151 GATGCCGCCA CGCCGTATGG CGAACCGCTG CCCGACCACT TCCTCGGCGC 201 GGCGCAAATG GTTCATGAAC TCGACCTGCT CCCCGATGCC GTCGCCGCCA 251 CCCTGCTTGC CGACATCGGA CGCTACGTCC CCGACTGGAA CCTATTGGTT 301 TCCGAACGCT GCAACAGTAC CGTCGCCGAG CTGGTCAAAG GTGTGGACGA 351 AGTGCAGAAA CTCACCCACT TCGCCCGGGT GGACAGCCTC GCCACGCCGG 401 AAGAACGCGC CCAGCAGGCA GAAACTATGC GGAAAATGCT GCTGGCGATG 451 GTTACCGACA TCCGCGTCGT GTTAATCAAA CTGGCGATGC GTACGCGCAC 501 CCTGCAATTT TTAAGCAACG CCCCCGACAG CCCCGAAAAA CGCGCCGTCG 551 CCAAAGAAAC CCTCGACATC TTCGCCCCGC TCGCCAACCG TTTGGGCGTG 601 TGGCAGCTCA AATGGCAGCT CGAAGATTTG GGCTTCCGCC ATCAAGAACC 651 CGAAAAATAC CGCGAAATCG CCCTGCTTTT GGACGAAAAA CGCACCGAAC 701 GCCTCGAATA CATCGAAAAC TTCCTTAATA TCCTGCGTAC GGAACTCAAA 751 AAATACAATA TCCACTTTGA AGTCGCCGGC CGTCCGAAAC ACATCTACTC 801 CATTTACAAA AAAATGGTGA AGAAAAAACT CAGCTTCGAC GGGTTGTTCG 851 ACATCCGCGC CGTGCGGATT CTGGTTGATA CCGTCCCCGA GTGTTACACC 901 ACACTGGGCA TTGTCCACAG CCTCTGGCAG CCCATTCCCG GCGAGTTCGA 951 CGACTACATC GCCAACCCGA AAGGCAACGG CTATAAAAGT TTGCACACCG 1001 TCATCGTCGG CCCGGAAGAC AAAGGCGTGG AAGTGCAAAT CCGCACCTTC 1051 GATATGCACC AATTCAACGA ATTCGGTGTC GCCGCGCACT GGCGTTACAA 1101 AGAGGGCGGC AAAGGCGATT CCGCCTACGA ACAAAAAATC GCCTGGTTAC 1151 GCCAACTTTT GGACTGGCGC GAAAACATGG CGGAAAGCGG CAAGGAAGAC 1201 CTCGCCGCCG CCTTCAAAAC CGAGCTTTTC AACGACACGA TTTATGTTTT 1251 GACCCCGCAC GGCAAAGTCC TCTCCCTGCC CACAGGCGCG ACCCCCATCG 1301 ACTTCGCCTA CGCCCTGCAC AGCAGCATCG GCGACCGTTG CCGCGGTGCG 1351 AAAGTCGAAG GGCAGATTGT GCCGCTGTCC ACCCCGCTCG AAAACGGACA 1401 GCGTGTCGAA ATCATTACCG CCAAAGAAGG GCATCCTTCC GTCAACTGGC 1451 TTTACGAAGG CTGGGTCAAA TCCAACAAGG CAATCGGCAA AATCCGCGCC 1501 TACATCCGCC AGCAAAACGC CGACACCGTG CGCGAAGAAG GCCGCGTCCA 1551 ACTCGACAAA CAGCTTGCCA AACTCACGCC CAAACCCAAC CTGCAAGAGC 1601 TTGCCGAAAA TCTCGGCTAC AAAAAGCCAG AAGACCTCTA CACCGCCGTC 1651 GGACAAGGCG AAATTTCCAA CCGCGCCATC CAAAAAGCCT GCGGCACGCT 1701 GAACGAACCG CCGCCCGTAC CCGTCAGCGA AACCACCATC GTCAAACAGT 1751 CCAAAATCAA AAAAGGCGGC AAAAACGGCG TGCTCATCGA CGGCGAAGAC 1801 GGTCTGATGA CCACGCTTGC CAAATGCTGC AAACCCGCGC CGCCCGACGA 1851 CATTGTCGGC TTCGTTACCC GCGATCGCGG CATTTCGGTA CACCGCAAAA 1901 CCTGCCCCTC TTTCCGACAC CTCGCCGAAC ACGCGCCCGA AAAAGTACTG 1951 GACGCAAGTT GGGCGGCGTT GCAGGAAGGA CAAGTGTTCG CCGTCGATAT 2001 CGAAATCCGC GCCCAAGACC GCTCCGGGCT TTTGCGCGAC GTATCCGACG 2051 CGCTCGCCCG CCACAAACTC AACGTTACCG CCGTGCAAAC CCAGTCCCGC 2101 GACTTGGAAG CCAGCATGAG GTTCACGCTC GAAGTCAAAC AAGTTACCGA 2151 CCTCCCACGC GTCCTCGCCA GCCTCGGCGA CGTCAAAGGC GTATTGAGCG 2201 TTACCCGGCT TTAA This corresponds to the amino acid sequence <SEQ ID 422; ORF 117-1.a>: all7-l.pep 1 MTAIΞPIQDT QSATLQELRE FDSYCTALP NNDKKLVLAA RSLAEAHYPA 51 DAATPYGEPL PDHFLGAAQM VHELDLLPDA VAATLLADIG RYVPD NLLV 101 SERCNSTVAE LVKGVDEVQK LTHFARVDΞL ATPEERAQQA ETMRKMLLAM 151 VTDIRVVLIK LAMRTRTLQF LSNAPDΞPEK RAVAKETLDI FAPLANRLGV 201 WQLKWQLEDL GFRHQEPEKY REIALLLDEK RTERLEYIEN FLNILRTELK 251 KYNIHFEVAG RPKHIYSIYK KMVKKKLSFD GLFDIRAVRI LVDTVPECYT 301 TLGIVHΞL Q PIPGEFDDYI ANPKGNGYKS LHTVIVGPED KGVEVQIRTF 351 DMHQFNEFGV AAHWRYKEGG KGDSAYEQKI AWLRQLLD R ENMAEΞGKED 401 LAAAFKTELF NDTIYVLTPH GKVLSLPTGA TPIDFAYALH SSIGDRCRGA 451 KVEGQIVPLS TPLENGQRVE IITAKEGHPS VN LYEGWVK ΞNKAIGKIRA 501 YIRQQNADTV REEGRVQLDK QLAKLTPKPN LQELAENLGY KKPEDLYTAV 551 GQGEISNRAI QKACGTLNEP PPVPVSETTI VKQSKIKKGG KNGVLIDGED 601 GLMTTLAKCC KPAPPDDIVG FVTRDRGISV HRKTCPSFRH LAEHAPEKVL 651 DASWAALQEG QVFAVDIEIR AQDRSGLLRD VSDALARHKL NVTAVQTQSR 701 DLEASMRFTL EVKQVTDLPR VLASLGDVKG VLSVTRL* all7-l/mll7-l 97.7% identity in 737 aa overlap 10 20 30 40 50 60 ml17-1. pep MTAISPIQDTQSATLQELRE FDSYCAALPDNDKNLIGTA LLAQEHYPADAATPYGEPL I I I I I I I I I I I I I I I II I I I I I I I I hi I 1:111:1: :| II: I I I I I I I I I I I I I I all7-l MTAISPIQDTQΞATLQELRE FDSYCTALPNNDKKLVLAARSLAEAHYPADAATPYGEPL 10 20 30 40 50 60 70 80 90 100 110 120 mll7-l.pep PDHFLGAAQMVHELDLLPDAVAATLLADIGRYVPD NLLVSERCNSTVAELVKGVDEVQK I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I all7-l PDHFLGAAQMVHELDLLPDAVAATLLADIGRYVPDWNLLVSERCNSTVAELVKGVDEVQK 70 80 90 100 110 120 130 140 150 160 170 180 ml17-1. pep LTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIKLAMRTRTLQFLSNAPDSPEK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I all7-1 LTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIKLAMRTRTLQFLSNAPDSPEK 130 140 150 160 170 180 190 200 210 220 230 240 ml17-1. pep RAVAKETLDIFAPLANRLGVWQLK QLEDLGFRHQKPEKYREIALLLDEKRTERLEYIEN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I: I I I I I I I I I I I I I I I I I I I I I I I I a117-1 RAVAKETLDIFAPLANRLGV QLKWQLEDLGFRHQEPEKYREIALLLDEKRTERLEYIEN 190 200 210 220 230 240 250 260 270 280 290 300 ml17-1. pep FLNILRGELKKYNVHFEVAGRPKHIYSIYKKMVKKKLΞFDGLFDIRAVRILVDTVPECYT l l l l l l I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I all7-l FLNILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKKLSFDGLFDIRAVRILVDTVPECYT 250 260 270 280 290 300 310 320 330 340 350 360 ml17-1. pep TLGIVHSL QPIPGEFDDYIANPKGNGYKSLHTVIVGPEDKGVEVQIRTFDMHQFNEFGV I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I all7-l TLGIVHSL QPIPGEFDDYIANPKGNGYKSLHTVIVGPEDKGVEVQIRTFDMHQFNEFGV 310 320 330 340 350 360 370 380 390 400 410 420 ml17-1.pep AAHWRYKEGGKGDSAYEQKIA LRQLLDWRENMAESGKEDLAAAFKTELFNDTIYVLTPH I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I all7-l AAHWRYKEGGKGDSAYEQKIAWLRQLLDWRENMAESGKEDLAAAFKTELFNDTIYVLTPH 370 380 390 400 410 420 430 440 450 460 470 480 ml17-1. pep GKVLSLPTGATPIDFAYALHSSIGDRCRGAKVEGQIVPLSTPLENGQRVEIITAKEGHPS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I all7-l GKVLSLPTGATPIDFAYALHSSIGDRCRGAKVEGQIVPLSTPLENGQRVEIITAKEGHPS 430 440 450 460 470 480 490 500 510 520 530 540 ml17-1. pep VN LYEGWVKSNKAIGKIRAYIRQQNADTVREEGRVQLDKQLAKLTPKPNLQELAENLGY I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I all7-l VNWLYEGWVKSNKAIGKIRAYIRQQNADTVREEGRVQLDKQLAKLTPKPNLQELAENLGY 490 500 510 520 530 540 550 560 570 580 590 600 mll7-l.pep KKPEDLYTAVGQGEISNRAIQKACGTLNEPPPVPVSETTIVKQSKIKKGGKNGVLIDGED I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al17-1 KKPEDLYTAVGQGEISNRAIQKACGTLNEPPPVPVΞETTIVKQSKIKKGGKNGVLIDGED 550 560 570 580 590 600 610 620 630 640 650 660 ml17-1. pep GLMTTLAKCCKPAPPDDIIGFVTRERGISVHRKTCPSFQHLAEHAPEKVLDAS AALQEG I I I I I I I I I I I I I I I I I I :| I I I I :| I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I all7-l GLMTTLAKCCKPAPPDDIVGFVTRDRGISVHRKTCPSFRHLAEHAPEKVLDAS AALQEG 610 620 630 640 650 660 670 680 690 700 710 720 ml17-1. pep QVFAVDIEIRAQDRSGLLRDVSDALARHKLNVTAVQTQSRDLEAΞMRFTLEVKQVNDLPR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I :| I I I all7-l QVFAVDIEIRAQDRΞGLLRDVSDALARHKLNVTAVQTQSRDLEASMRFTLEVKQVTDLPR 670 680 690 700 710 720 730 mll7-l .pep VLASLGDVKGVLSVTRLX I I I I I I I I I I I I I I I I I I all7-l VLASLGDVKGVLSVTRLX 730 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 423>: gll8. seq 1 ATGTGCGAGT TCAAGGATTT TAGAAGAAAC ATCCCTTGTT TTGAAGAGTA 51 TGACGAAAAT TCATTTATTG GCAAATGGTA TGATGACGGG GTGTGGGATG 101 ATGAAGAATA TTGGAAGCTG GAGAATGATT TAATcgaGGT TAGGAGAAAA 151 TATCCTTATC CGATGGATAT ACCAAGGGAT ATTGTGATTG GAATCGGTAC 201 CATTATTGAT TTTTTAATGG TTCCAAATTG GGAGCTTTTT GAAATTAAAG 251 CTTCCCCTTG GTTGCCTGAT AGCGTGGGAA TTCATGAACG TTATGAAAGA 301 TTCACAACGA TGCTCCGTTA TATTTTTACC GAGAAAGACA TAGTCAACGT 351 GCGATTTGAT TATTACAaCA AAAAATAG This corresponds to the amino acid sequence <SEQ ID 424; ORF 118.ng>: gllδ .pep 1 MCEFKDFRRΝ IPCFEEYDEΝ SFIGKWYDDG VWDDEEYWKL EΝDLIEVRRK 51 YPYPMDIPRD IVIGIGTIID FLMVPΝWELF EIKASPWLPD SVGIHERYER 101 FTTMLRYIFT EKDIVΝVRFD YYΝKK* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 425>: mll8. seq 1 ATGTGTGAGT TCAAGGATAT TATAAGAAAC GTTCCTTATT TTGAGGGGTA 51 TGACGAAAAT TCATTTATTG GCAAATGGTA TGATGACGGG GTGTGGGATG 101 ATGAAGAATA TTGGAAGTTG GAGAATGATT TAATCGAGGT TAGAAAAAAA 151 TATCCTTATC CGATGGACAT ACCAAGATAT GTTGTCATTG GAATCGGTAC 201 CATTATTGAT TTCTTAATGG TTCCAAATTG GAAACTTTTT GAAATTAAAG 251 CTTCCCCTTG GTTGCCTGAT AGTGTGGGAA TTCATGAACG TTATGAAAGA 301 TTCACAACGA TGCTCCGTTA TATTTTTACC GAGAAAGACA TAGTCAACGT 351 GCGATTTGAT TATTACAACA AAAAATAG This corresponds to the amino acid sequence <SEQ ID 426; ORF 118>: ml18.pep 1 MCEFKDIIRΝ VPYFEGYDEΝ SFIGKWYDDG VWDDEEYWKL EΝDLIEVRKK 51 YPYPMDIPRY VVIGIGTIID FLMVPΝWKLF EIKASPWLPD SVGIHERYER 101 FTTMLRYIFT EKDIVΝVRFD YYΝKK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 118 shows 92.8% identity over a 125 aa overlap with a predicted ORF (ORF 118.ng) from N gonorrhoeae: mll8/gll8 10 20 30 40 50 60 mll8. pep MCEFKDIIRΝVPYFEGYDEΝSFIGKWYDDGVWDDEEYWKLEΝDLIEVRKKYPYPMDIPRY l l l l l l: I I: I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I gll8 MCEFKDFRRΝIPCFEEYDEΝSFIGKWYDDGVWDDEEYWKLEΝDLIEVRRKYPYPMDIPRD 10 20 30 40 50 60 70 80 90 100 110 120 ml18. pep VVIGIGTIIDFLMVPΝWKLFEIKASPWLPDSVGIHERYERFTTMLRYIFTEKDIVΝVRFD : I I I I I I I I I I I I I I I I : II I I I I II I I I I I I I II I I I I I I I I I I I II I I I I I I I I I I I I gll8 IVIGIGTIIDFLMVPΝWELFEIKASPWLPDSVGIHERYERFTTMLRYIFTEKDIVΝVRFD 70 80 90 100 110 120 ml18. pep YYΝKKX llllll gll8 YYΝKKX The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 427>: all8. seq 1 ATGTGTGAGT TCAAGGATTT TAGAAGAAAC ATCCCTTGTT TTGAAGAGTA 51 TGACGAAAAT TCATTTATTG GCAAATGGTA TGATGACGGG GTGTGGGATG 101 ATGAAGAATA TTGGAAATTG GAGAATGATT TAATCGAGGT TAGAAAAAAA 151 TATCCTTATC CGATGGATAT ACCAAGGGAT ATTGTGATTG GAATCGGTAC 201 CATTATTGAT TTTTTAATGG TTCCAAATTG GGAGCTTTTT GAAATTAAAG 251 CTTCCCCTTG GTTGCCTGAT AGTGTGGGAA TTCATGAACG TTATGAAAGA 301 TTCACAACGA TGCTCCGTTA TATTTTTACC GAGAAAGACA TAGTCAACGT 351 GCGATTTGAT TATTACAACA AAAAATAG This corresponds to the amino acid sequence <SEQ ID 428; ORF 118.a>: allβ.pep 1 MCEFKDFRRΝ IPCFEEYDEΝ SFIGKWYDDG VWDDEEYWKL EΝDLIEVRKK 51 YPYPMDIPRD IVIGIGTIID FLMVPΝWELF EIKASPWLPD SVGIHERYER 101 FTTMLRYIFT EKDIVΝVRFD YYΝKK* mll8/all8 93.6% identity in 125 aa overlap 10 20 30 40 50 60 mil8. pep MCEFKDIIRΝVPYFEGYDEΝSFIGKWYDDGVWDDEEYWKLEΝDLIEVRKKYPYPMDIPRY l l l l l l: M: l M I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M I I I I I all8 MCEFKDFRRΝIPCFEEYDEΝSFIGKWYDDGVWDDEEYWKLEΝDLIEVRKKYPYPMDIPRD 10 20 30 40 50 60 70 80 90 100 110 120 mllδ.pep VVIGIGTIIDFLMVPNWKLFEIKASPWLPDSVGIHERYERFTTMLRYIFTEKDIVNVRFD : I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I all8 IVIGIGTIIDFLMVPNWELFEIKASPWLPDSVGIHERYERFTTMLRYIFTEKDIVNVRFD 70 80 90 100 110 120 ml18. pep YYNKKX all8 YYNKKX The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 429>: gl20.seq 1 ATGATGAAGA CTTTTAAAAA TATATTTTCC GCCGCCATTT TGTCCGCCGC 51 CCTGCCGTGC GCGTATGCGG CAAGGCTACC CCAATCCGCC GTGCTGCACT 101 ATTCCGGCAG CTACGGCATT CCCGCCACGA TGACATTTGA ACGCAGCGGC 151 AATGCTTACA AAATCGTTTC GACGATTAAA GTGCCGCTAT ACAATATCCG 201 TTTCGAATCC GGCGGTACGG TTGTCGGCAA TACCCTGCAC CCTGCCTACT 251 ATAAAGACAT ACGCAGGGGC AAACTGTATG CGGAAGCCAA ATTCGCCGAC 301 GGCAGCGTAA CCTACGGCAA AGCGGGCGAG AGCAAAACCG AGCAAAGCCC 351 CAAGGCTATG GATTTGTTCA CGCTTGCCTG GCAGTTGGCG GCAAATGACG 401 CGAAACTCCC CCCGGGTCTG AAAATCACCA ACGGCAAAAA ACTTTATTCC 451 GTCGGCGGCC TGAATAAGGC GGGTACGGGA AAATACAGCA Taggcggcgt 501 gGAAACCGAA GTCGTCAAAT ATCGGGTGCG GCGCGGCGAC GATACGGTAA 551 CGTATTTCTT CGCACCGTCC CTGAACAATA TTCCGGCACA AATCGGCTAT 601 ACCGAcgaCG GCAAAACCTA TACGCTGAAG CTCAAATCGG TGCAGATCAA 651 CGGACAGGCC GCCAAACCGT AA This corresponds to the amino acid sequence <SEQ ID 430; ORF 120.ng>: gl20.pep 1 MMKTFKΝIFS AAILSAALPC AYAARLPQSA VLHYSGSYGI PATMTFERSG 51 ΝAYKIVSTIK VPLYΝIRFES GGTWGΝTLH PAYYKDIRRG KLYAEAKFAD 101 GSVTYGKAGE SKTEQSPKAM DLFTLAWQLA AΝDAKLPPGL KITΝGKKLYS 151 VGGLΝKAGTG KYSIGGVETE WKYRVRRGD DTVTYFFAPS LΝΝIPAQIGY 201 TDDGKTYTLK LKSVQIΝGQA AKP* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 431>: ml20. seq 1 ATGATGAAGA CTTTTAAAAA TATATTTTCC GCCGCCATTT TGTCCGCCGC 51 CCTGCCGTGC GCGTATGCGG CAGGGCTGCC CCAATCCGCC GTGCTGmACT 101 ATTCCGGCAG CTACGGCATT CCCGCCACGA TGACATTTGA ACGCAGCGGC 151 AATGCTTACA AAATCGTTTC GACGATTAAA GTGCCGCTAT ACAATATCCG 201 TTTCGAGTCC GGCGGTACGG TTGTCGGCAA TACCCTGCAC CCTACCTACT 251 ATAGAGACAT ACGCAGGGGC AAACTGTATG CGGAAGCCAA ATTCGCCGAC 301 GGCAGCGTAA CTTACGGCAA AGCGGGCGAG AGCAAAACCG AGCAAAGCCC 351 CAAGGCTATG GATTTGTTCA CGCTTGCCTG GCAGTTGGCG GCAAATGACG 401 CGAAACTCCC CCCGGGGCTG AAAATCACCA ACGGCAAAAA ACTTTATTCC 451 GTCGGCGGTT TGAATAAGGC GGGTACAGGA AAATACAGCA TAGGCGGCGT 501 GGAAACCGAA GTCGTCAAAT ATCGGGTGCG GCGCGGCGAC GATGCGGTAA 551 TGTATTTCTT CGCACCGTCC CTGAACAATA TTCCGGCACA AATCGGCTAT 601 ACCGACGACG GCAAAACCTA TACGCTGAAA CTCAAATCGG TGCAGATCAA 651 CGGCCAGGCA GCCAAACCG This corresponds to the amino acid sequence <SEQ ID 432; ORF 120>: ml20.pep 1 MMKTFKΝIFS AAILSAALPC AYAAGLPQSA VLXYSGSYGI PATMTFERSG 51 ΝAYKIVSTIK VPLYΝIRFES GGTWGΝTLH PTYYRDIRRG KLYAEAKFAD 101 GSVTYGKAGE SKTEQSPKAM DLFTLAWQLA AΝDAKLPPGL KITΝGKKLYS 151 VGGLΝKAGTG KYSIGGVETE WKYRVRRGD DAVMYFFAPS LΝΝIPAQIGY 201 TDDGKTYTLK LKSVQIΝGQA AKP Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 120 shows 97.3% identity over a 223 aa overlap with a predicted ORF (ORF 120.ng) from N gonorrhoeae: ml20/gl20 10 20 30 40 50 60 ml20. pep MMKTFKΝIFSAAILSAALPCAYAAGLPQSAVLXYSGSYGIPATMTFERSGΝAYKIVSTIK 1111111 1111111111111 I 1111111111111 gl20 MMKTFKΝIFSAAILSAALPCAYAARLPQSAVLHYSGSYGIPATMTFERSGΝAYKIVSTIK 10 20 30 40 50 60 70 80 90 100 110 120 ml20.pep VPLYΝIRFESGGTWGΝTLHPTYYRDIRRGKLYAEAKFADGSVTYGKAGESKTEQSPKAM gl20 VPLYΝIRFESGGTWGΝTLHPAYYKDIRRGKLYAEAKFADGSVTYGKAGESKTEQSPKAM 70 80 90 100 110 120 130 140 150 160 170 180 ml20 . pep DLFTLAWQLAAΝDAKLPPGLKITΝGKKLYSVGGLΝKAGTGKYSIGGVETEWKYRVRRGD gl20 DLFTLAWQLAAΝDAKLPPGLKITΝGKKLYSVGGLΝKAGTGKYSIGGVETEWKYRVRRGD 130 140 150 160 170 180 190 200 210 220 ml20. pep DAVMYFFAPSLΝΝIPAQIGYTDDGKTYTLKLKSVQIΝGQAAKP gl20 DTVTYFFAPSLΝΝIPAQIGYTDDGKTYTLKLKSVQIΝGQAAKPX 190 200 210 220 The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 433>: al20.seq 1 ATGATGAAGA CTTTTAAAAA TATATTTTCC GCCGCCATTT TGTCCGCCGC 51 CCTGCCGTGC GCGTATGCGG CAGGGCTGCC CCAATCCGCC GTGCTGCACT 101 ATTCCGGCAG CTACGGCATT CCCGCCACGA TGACATTTGA ACGCAGCGGC 151 AATGCTTACA AAATCGTTTC GACGATTAAA GTGCCGCTAT ACAATATCCG 201 TTTCGAGTCC GGCGGTACGG TTGTCGGCAA TACCCTGCAC CCTACCTACT 251 ATAGAGACAT ACGCAGGGGC AAACTGTATG CGGAAGCCAA ATTCGCCGAC 301 GGCAGCGTAA CCTACGGCAA AGCGGGCGAG AGCAAAACCG AGCAAAGCCC 351 CAAGGCTATG GATTTGTTCA CGCTTGCCTG GCAGTTGGCG GCAAATGACG 401 CGAAACTCCC CCCGGGGCTG AAAATCACCA ACGGCAAAAA ACTTTATTCC 451 GTCGGCGGTT TGAATAAGGC GGGTACAGGA AAATACAGCA TAGGCGGCGT 501 GGAAACCGAA GTCGTCAAAT ATCGGGTGCG GCGCGGCGAC GATGCGGTAA 551 TGTATTTCTT CGCACCGTCC CTGAACAATA TTCCGGCACA AATCGGCTAT 601 ACCGACGACG GCAAAACCTA TACGCTGAAA CTCAAATCGG TGCAGATCAA 651 CGGCCAGGCA GCCAAACCGT AA This corresponds to the amino acid sequence <SEQ ID 434; ORF 120.a>: al20.pep 1 MMKTFKΝIFS AAILSAALPC AYAAGLPQSA VLHYSGSYGI PATMTFERSG 51 ΝAYKIVSTIK VPLYΝIRFES GGTWGΝTLH PTYYRDIRRG KLYAEAKFAD 101 GSVTYGKAGE SKTEQSPKAM DLFTLAWQLA AΝDAKLPPGL KITΝGKKLYS 151 VGGLΝKAGTG KYSIGGVETE WKYRVRRGD DAVMYFFAPS LΝΝIPAQIGY 201 TDDGKTYTLK LKSVQIΝGQA AKP* ml20/al20 99.6% identity in 223 aa overlap 10 20 30 40 50 60 ml20.pep MMKTFKΝIFSAAILSAALPCAYAAGLP Q SAVLXYSGSYGIPATMTFERSGΝAYKIVSTIK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al20 MMKTFKΝIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATMTFERSGΝAYKIVSTIK 10 20 30 40 50 60 70 80 90 100 110 120 ml20.pep VPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGSVTYGKAGESKTEQSPKAM I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al20 VPLYNIRFESGGTWGNTLHPTYYRDIRRGKLYAEAKFADGSVTYGKAGESKTEQSPKAM 70 80 90 100 110 120 130 140 150 160 170 180 ml20 . pep DLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTGKYSIGGVETEWKYRVRRGD I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al20 DLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTGKYSIGGVETEVVKYRVRRGD 130 140 150 160 170 180 190 200 210 220 ml20.pep DAVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQINGQAAKPX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al20 DAVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQINGQAAKPX 190 200 210 220 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 435>: gl21.seq 1 ATGGAAACAC AGCTTTACAT CGGCATTATG TCGGGAACCA GTATGGACGG 51 GGCGGATGCC GTGCTGGTAC GGATGGACGG CGGCAAATGG CTGGGCGCGG 101 AAGGGCACGC CTTTACCCCC TACCCTGACC GGTTGCGCCG CAAATTGCTG 151 GATTTGCAGG ACACAGGCAC AGACGAACTG CACCGCAGCA GGATGTTGTC 201 GCAAGAACTC AGCCGCCTGT ACGCGCAAAC CGCCGCCGAA CTGCTGTGCA 251 GTCAAAACCT CGCTCCGTGC GACATTACCG CCCTCGGCTG CCACGGGCAA 301 ACCGTCCGAC ACGCGCCGGA ACACGGTtac AGCATACAGC TTGCCGATTT 351 GCCGCTGCTG GCGGAACTGa cgcggatttT TACCGTCggc gacttcCGCA 401 GCCGCGACCT TGCTGCCGGC GGacaAGGTG CGCCGCTCGT CCCCGCCTTT 451 CACGAAGCCC TGTTCCGCGA TGACAGGGAA ACACGCGTGG TACTGAACAT 501 CGGCGGGATT GCCAACATCA GCGTACTCCC CCCCGGCGCA CCCGCCTTCG 551 GCTTCGACAC AGGGCCGGGC AATATGCTGA TGGAcgcgtg gacgcaggca 601 cacTGGcagc TGCCTTACGA CAAAAacggt gcAAAGgcgg cacAAGGCAA 651 catatTGCcg CAACTGCTCG gcaggctGCT CGCCcaccCG TATTTCTCAC 701 AACCCcaccc aaAAAGCACG GGgcGCGaac TgtttgcccT AAattggctc 751 gaaacctAcc ttgacggcgg cgaaaaccga tacgacgtat tgcggacgct 801 ttcccgattc accgcgcaaA ccgTttggga cgccgtctca CACGCAGCGG 851 CAGATGCCCG TCAAATGTAC ATTTGCGGCG GCGGCATCCG CAATCCTGTT 901 TTAATGGCGG ATTTGGCAGA ATGTTTCGGC ACACGCGTTT CCCTGCACAG 951 CACCGCCGAA CTGAACCTCG ATCCTCAATG GGTGGAGGCG gccgCATTtg 1001 cgtggttggC GGCGTGTTGG ATTAACCGCA TTCCCGGTAG TCCGCACAAA 1051 GCGACCGGCG CATCCAAACC GTGTATTCTG GGCGCGGGAT ATTATTATTG 1101 A This corresponds to the amino acid sequence <SEQ ID 436; ORF 121.ng>: gl21.pep 1 METQLYIGIM SGTSMDGADA VLVRMDGGKW LGAEGHAFTP YPDRLRRKLL 51 DLQDTGTDEL HRSRMLSQEL SRLYAQTAAE LLCSQΝLAPC DITALGCHGQ 101 TVRHAPEHGY SIQLADLPLL AELTRIFTVG DFRSRDLAAG GQGAPLVPAF 151 HEALFRDDRE TRVVLΝIGGI AΝISVLPPGA PAFGFDTGPG ΝMLMDAWTQA 201 HWQLPYDKΝG AKAAQGΝILP QLLGRLLAHP YFSQPHPKST GRELFALΝWL 251 ETYLDGGENR YDVLRTLSRF TAQTVWDAVS HAAADARQMY ICGGGIRNPV 301 LMADLAECFG TRVSLHSTAE LNLDPQWVEA AAFAWLAACW INRIPGSPHK 351 ATGASKPCIL GAGYYY* The following partial DNA sequence was identified in N meningitidis <SEQ ID 437>: ml21.seq 1 ATGGAAACAC AGCTTTACAT CGGCATCATG TCGGGAACCA GCATGGACGG 51 GGCGGATGCC GTACTGATAC GGATGGACGG CGGCAAATGG CTGGGCGCGG 101 AAGGGCACGC CTTTACCCCC TACCCCGGCA GGTTACGCCG CCAATTGCTG 151 GATTTGCAGG ACACAGGCGC AGACGAACTG CACCGCAGCA GGATTTTGTC 201 GCAAGAACTC AGCCGCCTAT ATGCGCAAAC CGCCGCCGAA CTGCTGTGCA 251 GTCAAAACCT CGCACCGTCC GACATTACCG CCCTCGGCTG CCACGGGCAA 301 ACCGTCCGAC ACGCGCCGGA ACACGGTTAC AGCATACAGC TTGCCGATTT 351 GCCGCTGCTG GCGxxxxxxx xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx 401 xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx 451 xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx 501 xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx xxxxxxxxx xxxxxxxxxx 551 xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx 601 xxxxxxCAGC TTCCTTACGA CAAAAACGGT GCAAAGTCGG CACAAGGCAA 651 CATATTGCCG CAACTGCTCG ACAGGCTGCT CGCCCACCCG TATTTCGCAC 701 AACGCCACCC TAAAAGCACG GGGCGCGAAC TGTTTGCCAT AAATTGGCTC 751 GAAACCTACC TTGACGGCGG CGAAAACCGA TACGACGTAT TGCGGACGCT 801 TTCCCGTTTT ACCGCGCAAA CCGTTTGCGA CGCCGTCTCA CACGCAGCGG 851 CAGATGCCCG TCAAATGTAC ATTTGCGACG GCGGCATCCG CAATCCTGTT 901 TTAATGGCGG ATTTGGCAGA ATGTTTCGGC ACACGCGTTT CCCTGCACAG 951 CACCGCCGAC CTGAACCTCG ATCCGCAATG GGTGGAAGCC GCCGnATTTG 1001 CGTGGTTGGC GGCGTGTTGG ATTAATCGCA TTCCCGGTAG TCCGCACAAA 1051 GCAACCGGCG CATCCAAACC GTGTATTCTG AnCGCGGGAT ATTATTATTG 1101 A This corresponds to the amino acid sequence <SEQ ID 438; ORF 121>: ml21.pep 1 METQLYIGIM SGTSMDGADA VLIRMDGGKW LGAEGHAFTP YPGRLRRQLL 51 DLQDTGADEL HRΞRILSQEL SRLYAQTAAE LLCSQNLAPS DITALGCHGQ 101 TVRHAPEHGY SIQLADLPLL Axxxxxxxxx xxxxxxxxxx xxxxxxxxxx 151 xxxxxxxxx xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx xxxxxxxxxx 201 xxQLPYDKNG AKSAQGNILP QLLDRLLAHP YFAQRHPKST GRELFAINWL 251 ETYLDGGENR YDVLRTLSRF TAQTVCDAVS HAAADARQMY ICDGGIRNPV 301 LMADLAECFG TRVSLHSTAD LNLDPQWVEA AXFAWLAACW INRIPGSPHK 351 ATGASKPCIL XAGYYY* Computer analysis of the amino acid sequences gave the following results: Homology with a predicted ORF from N meningitidis menA with menB ORF 121 shows 73.5% identity over a 366 aa overlap with a predicted ORF (ORF121.ng) from N gonorrhoeae: ml21/gl21 10 20 30 40 50 60 ml21.pep METQLYIGIMSGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPGRLRRQLLDLQDTGADEL I II I I II I I I I I I I I I I I I II I: I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I : I I I gl21 METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDRLRRKLLDLQDTGTDEL 10 20 30 40 50 60 70 80 90 100 110 120 ml21.pep HRSRILSQELSRLYAQTAAELLCSQΝLAPSDITALGCHGQTVRHAPEHGYSIQLADLPLL I I I I : I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl21 HRSRMLSQELSRLYAQTAAELLCSQΝLAPCDITALGCHGQTVRHAPEHGYSIQLADLPLL 70 80 90 100 110 120 130 140 150 160 170 180 ml21. pep AXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX I : : : gl21 AELTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDDRETR LΝIGGIAΝISVLPPGA 130 140 150 160 170 180 190 200 210 220 230 240 ml21. pep XXXXXXXXXXXXXXXXXXXXXXQLPYDKΝGAKSAQGΝILPQLLDRLLAHPYFAQRHPKST : : I I I I I I I I I I : I I I I I I I I I I I I I I I I I I : I I I I I I gl21 PAFGFDTGPGΝMLMDAWTQAHWQLPYDKΝGAKAAQGΝILPQLLGRLLAHPYFSQPHPKST 190 200 210 220 230 240 250 260 270 280 290 300 ml21. pep GRELFAIΝWLETYLDGGEΝRYDVLRTLSRFTAQTVCDAVSHAAADARQMYICDGGIRΝPV I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I lllllll gl21 GRELFALΝWLETYLDGGEΝRYDVLRTLSRFTAQTVWDAVSHAAADARQMYICGGGIRΝPV 250 260 270 280 290 300 310 320 330 340 350 360 ml21.pep LMADLAECFGTRVSLHSTADLNLDPQWVEAAXFAWLAACWINRIPGSPHKATGASKPCIL I II I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I gl21 LMADLAECFGTRVSLHSTAELNLDPQWVEAAAFAWLAACWINRIPGSPHKATGASKPCIL 310 320 330 340 350 360 ml21. pep XAGYYYX llllll gl21 GAGYYYX The following partial DNA sequence was identified in N meningitidis <SEQ ID 439>: al21. seq 1 ATGGAAACAC AGCTTTACAT CGGCATCATG TCGGGAACCA GCATGGACGG 51 GGCGGATGCC GTACTGATAC GGATGGACGG CGGCAAATGG CTGGGCGCGG 101 AAGGGCACGC CTTTACCCCC TACCCCGGCA GGTTACGCCG CAAATTGCTG 151 GATTTGCAGG ACACAGGCGC GGACGAACTG CACCGCAGCA GGATGTTGTC 201 GCAAGAACTC AGCCGCCTGT ACGCGCAAAC CGCCGCCGAA CTGCTGTGCA 251 GTCAAAACCT CGCGCCGTCC GACATTACCG CCCTCGGCTG CCACGGGCAA 301 ACCGTCAGAC ACGCGCCGGA ACACAGTTAC AGCGTACAGC TTGCCGATTT 351 GCCGCTGCTG GCGGAACGGA CTCAGATTTT TACCGTCGGC GACTTCCGCA 401 GCCGCGACCT TGCGGCCGGC GGACAAGGCG CGCCGCTCGT CCCCGCCTTT 451 CACGAAGCCC TGTTCCGCGA CGACAGGGAA ACACGCGCGG TACTGAACAT 501 CGGCGGGATT GCCAACATCA GCGTACTCCC CCCCGACGCA CCCGCCTTCG 551 GCTTCGACAC AGGACCGGGC AATATGCTGA TGGACGCGTG GATGCAGGCA 601 CACTGGCAGC TTCCTTACGA CAAAAACGGT GCAAAGGCGG CACAAGGCAA 651 CATATTGCCG CAACTGCTCG ACAGGCTGCT CGCCCACCCG TATTTCGCAC 701 AACCCCACCC TAAAAGCACG GGGCGCGAAC TGTTTGCCCT AAATTGGCTC 751 GAAACCTACC TTGACGGCGG CGAAAACCGA TACGACGTAT TGCGGACGCT 801 TTCCCGATTC ACCGCGCAAA CCGTTTTCGA CGCCGTCTCA CACGCAGCGG 851 CAGATGCCCG TCAAATGTAC ATTTGCGGCG GCGGCATCCG CAATCCTGTT 901 TTAATGGCGG ATTTGGCAGA ATGTTTCGGC ACACGCGTTT CCCTGCACAG 951 CACCGCCGAA CTGAACCTCG ATCCGCAATG GGTAGAAGCC GCCGCGTTCG 1001 CATGGATGGC GGCGTGTTGG GTCAACCGCA TTCCCGGTAG TCCGCACAAA 1051 GCAACCGGCG CATCCAAACC GTGTATTCTG GGCGCGGGAT ATTATTATTG 1101 A This corresponds to the amino acid sequence <SEQ ID 440; ORF 121.a>: al21.pep 1 METQLYIGIM SGTSMDGADA VLIRMDGGKW LGAEGHAFTP YPGRLRRKLL 51 DLQDTGADEL HRSRMLSQEL SRLYAQTAAE LLCSQΝLAPS DITALGCHGQ 101 TVRHAPEHSY SVQLADLPLL AERTQIFTVG DFRSRDLAAG GQGAPLVPAF 151 HEALFRDDRE TRAVLΝIGGI AΝISVLPPDA PAFGFDTGPG ΝMLMDAWMQA 201 HWQLPYDKΝG AKAAQGΝILP QLLDRLLAHP YFAQPHPKST GRELFALΝWL 251 ETYLDGGENR YDVLRTLSRF TAQTVFDAVS HAAADARQMY ICGGGIRNPV 301 LMADLAECFG TRVSLHSTAE LNLDPQWVEA AAFAWMAACW VNRIPGSPHK 351 ATGASKPCIL GAGYYY* l21/al21 74.0% identity in 366 aa overlap 10 20 30 40 50 60 ml21. pep METQLYIGIMSGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPGRLRRQLLDLQDTGADEL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I al21 METQLYIGIMSGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPGRLRRKLLDLQDTGADEL 10 20 30 40 50 60 70 80 90 100 110 120 ml21. pep HRSRILS Q ELSRLYAQTAAELLCSQNLAPSDITALGCHGQTVRHAPEHGYSIQLADLPLL I I I I: I I I I I I I I I I I I I I I II I I I II I I I I I I I I I I I II I I I I I I I I: I I: I I I I I I I I al21 HRSRMLS Q ELSRLYA Q TAAELLCS Q NLAPSDITALGCHGQTVRHAPEHSYSVQLADLPLL 70 80 90 100 110 120 130 140 150 160 170 180 ml21. pep AXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX al21 AERTQIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDDRETRAVLNIGGIANISVLPPDA 130 140 150 160 170 180 190 200 210 220 230 240 ml21. pep XXXXXXXXXXXXXXXXXXXXXXQLPYDKNGAKSAQGNILPQLLDRLLAHPYFAQRHPKST : I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I al21 PAFGFDTGPGNMLMDAWMQAHWQLPYDKNGAKAAQGNILPQLLDRLLAHPYFAQPHPKST 190 200 210 220 230 240 250 260 270 280 290 300 ml21. pep GRELFAINWLETYLDGGENRYDVLRTLSRFTAQTVCDAVSHAAADARQMYICDGGIRNPV I I I I I I : I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I lllllll al21 GRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVFDAVSHAAADARQMYICGGGIRNPV 250 260 270 280 290 300 310 320 330 340 350 360 ml21.pep LMADLAECFGTRVSLHSTADLNLDPQWVEAAXFAWLAACWINRIPGSPHKATGASKPCIL I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I : I I I I : I I I I II II I I I I I I I I I I I al21 LMADLAECFGTRVSLHSTAELNLDPQWVEAAAFAWMAACWVNRIPGSPHKATGASKPCIL 310 320 330 340 350 360 ml21. pep XAGYYYX al21 GAGYYYX The following partial DNA sequence was identified in N. meningitidis <SEQ ID 441>: ml21-l.seq 1 ATGGAAACAC AGCTTTACAT CGGCATCATG TCGGGAACCA GCATGGACGG 51 GGCGGATGCC GTACTGATAC GGATGGACGG CGGCAAATGG CTGGGCGCGG 101 AAGGGCACGC CTTTACCCCC TACCCCGGCA GGTTACGCCG CCAATTGCTG 151 GATTTGCAGG ACACAGGCGC AGACGAACTG CACCGCAGCA GGATTTTGTC 201 GCAAGAACTC AGCCGCCTAT ATGCGCAAAC CGCCGCCGAA CTGCTGTGCA 251 GTCAAAACCT CGCACCGTCC GACATTACCG CCCTCGGCTG CCACGGGCAA 301 ACCGTCCGAC ACGCGCCGGA ACACGGTTAC AGCATACAGC TTGCCGATTT 351 GCCGCTGCTG GCGGAACGGA CGCGGATTTT TACCGTCGGC GACTTCCGCA 401 GCCGCGACCT TGCGGCCGGC GGACAAGGCG CGCCACTCGT CCCCGCCTTT 451 CACGAAGCCC TGTTCCGCGA CAACAGGGAA ACACGCGCGG TACTGAACAT 501 CGGCGGGATT GCCAACATCA GCGTACTCCC CCCCGACGCA CCCGCCTTCG 551 GCTTCGACAC AGGGCCGGGC AATATGCTGA TGGACGCGTG GACGCAGGCA 601 CACTGGCAGC TTCCTTACGA CAAAAACGGT GCAAAGGCGG CACAAGGCAA 651 CATATTGCCG CAACTGCTCG ACAGGCTGCT CGCCCACCCG TATTTCGCAC 701 AACCCCACCC TAAAAGCACG GGGCGCGAAC TGTTTGCCCT AAATTGGCTC 751 GAAACCTACC TTGACGGCGG CGAAAACCGA TACGACGTAT TGCGGACGCT 801 TTCCCGTTTT ACCGCGCAAA CCGTTTGCGA CGCCGTCTCA CACGCAGCGG 851 CAGATGCCCG TCAAATGTAC ATTTGCGGCG GCGGCATCCG CAATCCTGTT 901 TTAATGGCGG ATTTGGCAGA ATGTTTCGGC ACACGCGTTT CCCTGCACAG 951 CACCGCCGAC CTGAACCTCG ATCCGCAATG GGTGGAAGCC GCCGΝATTTG 1001 CGTGGTTGGC GGCGTGTTGG ATTAATCGCA TTCCCGGTAG TCCGCACAAA 1051 GCAACCGGCG CATCCAAACC GTGTATTCTG AΝCGCGGGAT ATTATTATTG 1101 A This corresponds to the amino acid sequence <SEQ ID 442; ORF 121-1>: l21-l.pep 1 METQLYIGIM SGTSMDGADA VLIRMDGGKW LGAEGHAFTP YPGRLRRQLL 51 DLQDTGADEL HRSRILSQEL SRLYAQTAAE LLCSQΝLAPS DITALGCHGQ 101 TVRHAPEHGY SIQLADLPLL AERTRIFTVG DFRSRDLAAG GQGAPLVPAF 151 HEALFRDΝRE TRAVLΝIGGI AΝISVLPPDA PAFGFDTGPG ΝMLMDA TQA 201 H QLPYDKΝG AKAAQGΝILP QLLDRLLAHP YFAQPHPKST GRELFALΝWL 251 ETYLDGGENR YDVLRTLSRF TAQTVCDAVS HAAADARQMY ICGGGIRNPV 301 LMADLAECFG TRVSLHΞTAD LNLDPQWVEA AXFAWLAACW INRIPGSPHK 351 ATGASKPCIL XAGYYY* ml21-l/gl21 95.6% identity in 366 aa overlap 10 20 30 40 50 60 ml21-l.pep METQLYIGIMΞGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPGRLRRQLLDLQDTGADEL I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I : I I II I I I I : I I I gl21 METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDRLRRKLLDLQDTGTDEL 10 20 30 40 50 60 70 80 90 100 110 120 ml21-l.pep HRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTVRHAPEHGYSIQLADLPLL I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl21 HRSRMLSQELSRLYAQTAAELLCSQNLAPCDITALGCHGQTVRHAPEHGYSIQLADLPLL 70 80 90 100 110 120 130 140 150 160 170 180 ml21-l.pep AERTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDNRETRAVLNIGGIANISVLPPDA II I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I : I I I I I I I I I I I I I I I I g121 AELTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDDRETRVVLNIGGIANISVLPPGA 130 140 150 160 170 180 190 200 210 220 230 240 ml21-l.pep PAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNILPQLLDRLLAHPYFAQPHPKST I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I gl21 PAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNILPQLLGRLLAHPYFSQPHPKΞT 190 200 210 220 230 240 250 260 270 280 290 300 ml21-l.pep GRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVCDAVSHAAADARQMYICGGGIRNPV I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl21 GRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVWDAVSHAAADARQMYICGGGIRNPV 250 260 270 280 290 300 310 320 330 340 350 360 ml21-l.pep LMADLAECFGTRVSLHΞTADLNLDPQWVEAAXFAWLAAC INRIPGΞPHKATGASKPCIL I I I I I I I I I I I I I I I I I I I :| I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl21 LMADLAECFGTRVSLHSTAELNLDPQWVEAAAFAWLAACWINRIPGSPHKATGASKPCIL 310 320 330 340 350 360 ml21-l .pep XAGYYYX llllll gl21 GAGYYYX The following partial DNA sequence was identified in N. meningitidis <SEQ ID 443>: al21-l.seq 1 ATGGAAACAC AGCTTTACAT CGGCATCATG TCGGGAACCA GCATGGACGG 51 GGCGGATGCC GTACTGATAC GGATGGACGG CGGCAAATGG CTGGGCGCGG 101 AAGGGCACGC CTTTACCCCC TACCCCGGCA GGTTACGCCG CAAATTGCTG 151 GATTTGCAGG ACACAGGCGC GGACGAACTG CACCGCAGCA GGATGTTGTC 201 GCAAGAACTC AGCCGCCTGT ACGCGCAAAC CGCCGCCGAA CTGCTGTGCA 251 GTCAAAACCT CGCGCCGTCC GACATTACCG CCCTCGGCTG CCACGGGCAA 301 ACCGTCAGAC ACGCGCCGGA ACACAGTTAC AGCGTACAGC TTGCCGATTT 351 GCCGCTGCTG GCGGAACGGA CTCAGATTTT TACCGTCGGC GACTTCCGCA 401 GCCGCGACCT TGCGGCCGGC GGACAAGGCG CGCCGCTCGT CCCCGCCTTT 451 CACGAAGCCC TGTTCCGCGA CGACAGGGAA ACACGCGCGG TACTGAACAT 501 CGGCGGGATT GCCAACATCA GCGTACTCCC CCCCGACGCA CCCGCCTTCG 551 GCTTCGACAC AGGACCGGGC AATATGCTGA TGGACGCGTG GATGCAGGCA 601 CACTGGCAGC TTCCTTACGA CAAAAACGGT GCAAAGGCGG CACAAGGCAA 651 CATATTGCCG CAACTGCTCG ACAGGCTGCT CGCCCACCCG TATTTCGCAC 701 AACCCCACCC TAAAAGCACG GGGCGCGAAC TGTTTGCCCT AAATTGGCTC 751 GAAACCTACC TTGACGGCGG CGAAAACCGA TACGACGTAT TGCGGACGCT 801 TTCCCGATTC ACCGCGCAAA CCGTTTTCGA CGCCGTCTCA CACGCAGCGG 851 CAGATGCCCG TCAAATGTAC ATTTGCGGCG GCGGCATCCG CAATCCTGTT 901 TTAATGGCGG ATTTGGCAGA ATGTTTCGGC ACACGCGTTT CCCTGCACAG 951 CACCGCCGAA CTGAACCTCG ATCCGCAATG GGTAGAAGCC GCCGCGTTCG 1001 CATGGATGGC GGCGTGTTGG GTCAACCGCA TTCCCGGTAG TCCGCACAAA 1051 GCAACCGGCG CATCCAAACC GTGTATTCTG GGCGCGGGAT ATTATTATTG 1101 A This corresponds to the amino acid sequence <SEQ ID 444; ORF 121-1. a>: al21-l.pep 1 METQLYIGIM SGTSMDGADA VLIRMDGGKW LGAEGHAFTP YPGRLRRKLL 51 DLQDTGADEL HRSRMLSQEL SRLYAQTAAE LLCSQΝLAPS DITALGCHGQ 101 TVRHAPEHSY SVQLADLPLL AERTQIFTVG DFRSRDLAAG GQGAPLVPAF 151 HEALFRDDRE TRAVLΝIGGI AΝISVLPPDA PAFGFDTGPG ΝMLMDAWMQA 201 HWQLPYDKNG AKAAQGNILP QLLDRLLAHP YFAQPHPKST GRELFALNWL 251 ETYLDGGENR YDVLRTLSRF TAQTVFDAVS HAAADARQMY ICGGGIRNPV 301 LMADLAECFG TRVSLHSTAE LNLDPQWVEA AAFAWMAACW VNRIPGSPHK 351 ATGASKPCIL GAGYYY* ml21-l/al21-l 96.4% identity in 366 aa overlap 10 20 30 40 50 60 ml21-l.pep METQLYIGIMSGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPGRLRRQLLDLQDTGADEL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I al21-l METQLYIGIMSGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPGRLRRKLLDLQDTGADEL 10 20 30 40 50 60 70 80 90 100 110 120 ml21-l.pep HRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTVRHAPEHGYSIQLADLPLL I I I I :| I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I :| I I I I I I I al21-l HRSRMLSQELSRLYAQTAAELLCΞQNLAPSDITALGCHGQTVRHAPEHSYΞVQLADLPLL 70 80 90 100 110 120 130 140 150 160 170 180 ml21-l.pep AERTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDNRETRAVLNIGGIANISVLPPDA I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I al21-l AERTQIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDDRETRAVLNIGGIANISVLPPDA 130 140 150 160 170 180 190 200 210 220 230 240 ml21-l.pep PAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNILPQLLDRLLAHPYFAQPHPKΞT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al21-l PAFGFDTGPGNMLMDAWMQAHWQLPYDKNGAKAAQGNILPQLLDRLLAHPYFAQPHPKST 190 200 210 220 230 240 250 260 270 280 290 300 ml21-l . pep GRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVCDAVSHAAADARQMYICGGGIRNPV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al21-l GRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVFDAVSHAAADARQMYICGGGIRNPV 250 260 270 280 290 300 310 320 330 340 350 360 ml21-1. pep LMADLAECFGTRVSLHΞTADLNLDPQWVEAAXFAWLAACWINRIPGSPHKATGASKPCIL I I I I I I I I I I I I I I I I I I I = 1 I I I I I I I I I I I I I: I I I I :l I I I I I I I I I I I I I I I I I I a121 LMADLAECFGTRVSLHSTAELNLDPQWVEAAAFAWMAACWVNRIPGSPHKATGASKPCIL 310 320 330 340 350 360 ml21-l.pep XAGYYYX llllll al21 GAGYYYX The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 445>: gl22.seq 1 ATGGCTTTAC TGAGCATCCG CAAGCTGCAC AAACAATACG GCAGCGTAAC 51 CGCCATCCAA TCCTTAGACT TGGACTTGGA AAAAGGCGAA GtcatCGTAC 101 TGCTGGGCCC gTccggctgc ggCAAATCCA CCCTcctgcg ctgcgtcaaC 151 GGTTTGGAGC CGCACCAagg cgGCAGCATC GTGATGGACG GTgtcgGCGA 201 ATTCggcAAA GACGTTTCCT GGCAAACCGC CCGGCAAAAa gtcggtatgg 251 tctttcaaag taacgAactg Tttgcccaca tgaccgtcat cgAaaacatc 301 ttcttAggcC CGGTAAagga aCAAAAcCgc gaccgtgccg aagcaGAGGC 351 gCAAGCCGGC AAactGttgg aacgcgTCGG actgctAGAC CGCAAAAACG 401 CCTATCCGCG CGAACTTTCC GGCGGTCAGA AACAGCGCAT CGCCATTGTC 451 CGCGCCCTGT GCCTGAATCC GGAAGTCATC CTGCTGGACG AAATCACCGC 501 CGCACTTGAC CCCGAAATGG TGCGCGAAGT CTTGGAAGTG GTTTTGGAAC 551 TCGCCCGCGA AGGGATGAGT ATGCTCATCG TAACCCACGA AATGGGGTTC 601 GCACGCAAAG TTGCCGACCG CATCGTCTTT ATGGACAAAG GCGGCATCGT 651 CGAATCGTCC GACCCCGAAA CCTTTTTTTC CGCACCAAAA AGCGAACGCG 701 CCCGCCAATT TCTGGCAGGT ATGGACTACT GA This corresponds to the amino acid sequence <SEQ ID 446; ORF 122.ng>: gl22.pep 1 MALLSIRKLH KQYGSVTAIQ SLDLDLEKGE VIVLLGPSGC GKSTLLRCVN 51 GLEPHQGGSI VMDGVGEFGK DVSWQTARQK VGMVFQSNEL FAHMTVIENI 101 FLGPVKEQNR DRAEAEAQAG KLLERVGLLD RKNAYPRELS GGQKQRIAIV 151 RALCLNPEVI LLDEITAALD PEMVREVLEV VLELAREGMS MLIVTHEMGF 201 ARKVADRIVF MDKGGIVESS DPETFFSAPK SERARQFLAG MDY* The following partial DNA sequence was identified in N meningitidis <SEQ ID 447>: ml22. seq 1 GTTGTCATGA TTAAAATCCG CAATATCCAT AAGACCTTTG GCGAAAACAC 51 TATTTTGCGC GGCATCGATT TGGATGTGTG CAAAGGGCAG GTGGTCGTCA 101 TCCTCGGGcC TTCCGGCTCA GGCAAAACGA CGTTTCTGCG ATGCCTAAAC 151 GCGTTGGAAA TGCCCGAAGA CGGACAAATC GAGTTCGACA ACGAGCGACC 201 GCTGAAAATC GATTTTTCTA AAAAACCAAG CAAACACGAT ATTTTGGCAC 251 TGCGCCGCAA ATCA GCATG GTGTTTCAAC AATACAAyCT CTTTCCGCAC 301 AAAACCGCCT TGGAAAACGT AATGGAAGGA CCGGTTGCCG TACAgGGCAA 351 GCCTGCCGCC CAAGCGCGCG AAGAGGCTCT GAAACTGCTG GAAAAAGTCG 401 GCTTGGGCGA CAAAGTGGAT TTGTATCCCT ACCAGCTTTC CGGCGGTCAG 451 CAGCAGCGCG TCGGCATTGC CCGCGCATTG GCGATTCAGC CTGAACTGAT 501 GCTGTTTGAC GAACCGACTT CCGCGCTCGA TCCTGAATTG GTGCAAGATG 551 TTTTGGATmC CATGAAGGAA TTGGCGCAAG AAGGCTGGAC CATGGTTGTC 601 GTTACGCATG AAATCAAGTT CGCCTTAGAA GTGGCAACCA CCGwCGTCGT 651 GATGGACrGC GGCGTTATTG TCGAACAAGG CAGCCCGCAA GATTTGTTCG 701 ACCACCCCAA ACACGAACGG ACGCGGAGAT TTTTAAGCCA AATCCAATCT 751 ACCAAGATTT GA This corresponds to the amino acid sequence <SEQ ID 448; ORF 122>: ml2 .pep 1 WMIKIRΝIH KTFGEΝTILR GIDLDVCKGQ VWILGPSGS GKTTFLRCLΝ 51 ALEMPEDGQI EFDΝERPLKI DFSKKPSKHD ILALRRKSXM VFQQYΝLFPH 101 KTALEΝVMEG PVAVQGKPAA QAREEALKLL EKVGLGDKVD LYPYQLSGGQ 151 QQRVGIARAL AIQPELMLFD EPTSALDPEL VQDVLDXMKE LAQEGWTMW 201 VTHEIKFALE VATTXWMDX GVIVEQGSPQ DLFDHPKHER TRRFLSQIQS 251 TKI* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 122 shows 47.2%> identity over a 246 aa overlap with a predicted ORF (ORF 122.ng) from N. gonorrhoeae: ml22/gl22 10 20 30 40 50 60 ml22. pep WMIKIRΝIHKTFGEΝTILRGIDLDVCKGQVWILGPSGSGKTTFLRCLΝALEMPEDGQI :::::| | : : || : | I : = : : | || : I hhhll l l l I hhl lhhl l = hi gl22 MALLSIRKLHKQYGSVTAIQSLDLDLEKGEVIVLLGPSGCGKSTLLRCVΝGLEPHQGGSI 10 20 30 40 50 60 70 80 90 100 110 120 ml2 .pep EFDΝERPLKIDFSKKPSKHDILALRRKSXMVFQQYΝLFPHKTALEΝVMEGPVAVQGKPAA = h = I I = = hi 1111= =11 I l-ll- III I- I g122 VMDGVGEFGKDVSWQTA RQKVGMVFQSΝELFAHMTVIEΝIFLGPVKEQΝRDRA 70 80 90 100 110 130 140 150 160 170 180 ml22 -pep QAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAIQPELMLFDEPTSALDPEL : h =1 l l l hl l l h = I I : I I I I h I h = h I I I : :|h:hl l 1 = 11111 = gl22 EAEAQAGKLLERVGLLDRKΝAYPRELSGGQKQRIAIVRALCLΝPEVILLDEITAALDPEM 120 130 140 150 160 170 190 200 210 220 230 240 ml22.pep VQDVLDXMKELAQEGWTMVWTHEIKFALEVATTXWMDXGVIVEQGSPQDLFDHPKHER \---A\-- ■■ llhll : h = |||h II =11 I II I llh::|: :h II II gl22 VREVLEWLELAREGMSMLIVTHEMGFARKVADRIVFMDKGGIVESSDPETFFSAPKSER 180 190 200 210 220 230 250 ml22.pep TRRFLSQIQSTKIX ■ 1 • 11 • gl22 ARQFLAGMDYX 240 The following partial DNA sequence was identified in N meningitidis <SEQ ID 449>: al22.seq 1 GTTGTCATGA TTAAAATCCG CAATATCCAT AAGACCTTCG GCAAAAATAC 51 CATTTTGCGC GGCATCAATT TGGATGTGTG CAAAGGGCAG GTGGTCGTCA 101 TCCTCGGGCC TTCCGGCTCA GGCAAAACGA CGTTTCTGCG ATGCCTAAAC 151 GCGTTGGAAA TGCCCGAAGA CGGACAAATC GAGTTCGACA ACGAGCGACC 201 GCTGAAAATC GATTTTTCTA AAAAACCAAG CAAACACGAT ATTTTGGCAC 251 TGCGCCGCAA ATCAGGCATG GTGTTTCAAC AATACAACCT CTTTCCGCAC 301 AAAACCGCCT TGGAAAACGT GATGGAAGGA CCGGTTGCCG TACAGGGCAA 351 GCCTGCCGCC CAAGCGCGCG AAGAGGCTCT GAAACTGCTG GAAAAAGTCG 401 GCTTGGGCGA CAAAGTGGAT TTGTATCCCT ACCAGCTTTC CGGCGGTCAG 451 CAGCAGCGCG TCGGCATTGC CCGAGCATTG GCGATTCAGC CCGAGCTGAT 501 GTTGTTTGAC GAACCCACTT CCGCGCTTGA CCCCGAGTTG GTGCAAGACG 551 TGTTGAACGC CATGAAGGAA TTGGCGCGGG AAGGTTGGAC GATGGTCGTC 601 GTTACCCACG AAATCAAGTT CGCGCTGGAA GTTGCCACGA CCGTTGTCGT 651 GATGGACGGC GGCGTTATCG TAGAGCAGGG CAGCCCGAAA GAGTTGTTCG 701 ACCACCCCAA ACACGAACGG ACGCGGAGAT TTTTAAGCCA AATCCAATCT 751 ACCAAGATTT GA This corresponds to the amino acid sequence <SEQ ID 450; ORF 122.a>: al22.pep 1 VVMIKIRΝIH KTFGKΝTILR GIΝLDVCKGQ VWILGPSGS GKTTFLRCLΝ 51 ALEMPEDGQI EFDΝERPLKI DFSKKPSKHD ILALRRKSGM VFQQYΝLFPH 101 KTALEΝVMEG PVAVQGKPAA QAREEALKLL EKVGLGDKVD LYPYQLSGGQ 151 QQRVGIARAL AIQPELMLFD EPTSALDPEL VQDVLΝAMKE LAREGWTMVV 201 VTHEIKFALE VATTVWMDG GVIVEQGSPK ELFDHPKHER TRRFLSQIQS 251 TKI* ml22/al22 96.0% identity in 253 aa overlap 10 20 30 40 50 60 ml22 . pep WMIKIRΝIHKTFGEΝTILRGIDLDVCKGQVWILGPSGSGKTTFLRCLΝALEMPEDGQI I I I I I I I I I I I I I I : I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al22 WMIKIRΝIHKTFGKΝTILRGIΝLDVCKGQWVILGPSGSGKTTFLRCLΝALEMPEDGQI 10 20 30 40 50 60 70 80 90 100 110 120 ml22 . pep EFDΝERPLKIDFSKKPSKHDILALRRKSXMVFQQYΝLFPHKTALEΝVMEGPVAVQGKPAA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al22 EFDΝERPLKIDFSKKPSKHDILALRRKSGMVFQQYΝLFPHKTALEΝVMEGPVAVQGKPAA 70 80 90 100 110 120 130 140 150 160 170 180 ml22. pep QAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAIQPELMLFDEPTSALDPEL I I I I I I I I I II I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al22 QAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAIQPELMLFDEPTSALDPEL 130 140 150 160 170 180 190 200 210 220 230 240 ml22. pep VQDVLDXMKELAQEGWTMVVVTHEIKFALEVATTXVVMDXGVIVEQGΞPQDLFDHPKHER Mill: I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I :: I I I I I I I II al22 VQDVLΝAMKELAREGWTMVVVTHEIKFALEVATTVWMDGGVIVEQGSPKELFDHPKHER 190 200 210 220 230 240 250 ml22.pep TRRFLSQIQSTKIX I I I I I I I I I I I I I I al22 TRRFLSQIQSTKIX 250 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 451 > • gl22-l.seq 1 ATGATTAAAA TCCGCAATAT CCATAAGACC TTTGGCGAAA ACACCATTTT 51 GCGCGGCATC GATTTGGATG TGGGCAAAGG GCAGGTGGTC GTCATCCTCG 101 GGCCTTCCGG CTCGGGTAAA ACAACATTTC TGCGCTGCCT AAACGCGTTG 151 GAAATGCCCG AAGACGGACA AATCGAGTTC GACAACGCGC GGCCGTTACG 201 CATTGATTTT TCCAAAAAAA CAAGCAAACA CGATATTTTG GCACTGCGCC 251 GCAAGTCCGG AATGGTATTC CAACAATACA ACCTCTTCCC GCATAAAACC 301 GTGTTGGAAA ACGTGATGGA AGGGCCGGTT GCCGTACAGG GCAAGCCTGC 351 CGCCCAAGCG CGCGAAGAGG CTTTGAAACT GCTGGAAAAA GTCGGCTTGG 401 GCGATAAAGT GGATTTGTAT CCCTACCAGC TTTCCGGCGG TCAGCAGCAG 451 CGTGTCGGTA TCGCCCGCGC ACTGGCGATT CAGCCTGAAT TGATGCTGTT 501 TGACGAACCC ACTTCCGCGC TGGACCCCGA GTTGGTGCAA GACGTGTTGG 551 ACGCCATGAA GGAATTGGCG CGGGAAGGTT GGACGATGGT CGTCGTTACC 601 CACGAAATCA AGTTCACGCT GGAAGTTGCC ACGAACGTCG TCGTGATGGA 651 CGGCGGCGTT ATCGTAGAGC AGGGCAGCCC GAAAGAGTTG TTCGACCACC 701 TCAAACACGA ACGGACGCGG AGATTTTTAA GCCAAATCCA ATCTGCCAAG 751 ATTTGA This corresponds to the amino acid sequence <SEQ ID 452; ORF 122-1. ng>: gl22-l.pep 1 MIKIRΝIHKT FGEΝTILRGI DLDVGKGQVV VILGPSGΞGK TTFLRCLΝAL 51 EMPEDGQIEF DΝARPLRIDF SKKTSKHDIL ALRRKSGMVF QQYΝLFPHKT 101 VLEΝVMEGPV AVQGKPAAQA REEALKLLEK VGLGDKVDLY PYQLSGGQQQ 151 RVGIARALAI QPELMLFDEP TSALDPELVQ DVLDAMKELA REGWTMVVVT 201 HEIKFTLEVA TΝVVVMDGGV IVEQGΞPKEL FDHLKHERTR RFLSQIQSAK 251 I* The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 453>: ml22-l.seq 1 ATGATTAAAA TCCGCAATAT CCATAAGACC TTTGGCGAAA ACACTATTTT 51 GCGCGGCATC GATTTGGATG TGTGCAAAGG GCAGGTGGTC GTCATCCTCG 101 GGCCTTCCGG CTCAGGCAAA ACGACGTTTC TGCGATGCCT AAACGCGTTG 151 GAAATGCCCG AAGACGGACA AATCGAGTTC GACAACGAGC GACCGCTGAA 201 AATCGATTTT TCTAAAAAAC CAAGCAAACA CGATATTTTG GCACTGCGCC 251 GCAAATCAGG CATGGTGTTT CAACAATACA ACCTCTTTCC GCACAAAACC 301 GCCTTGGAAA ACGTAATGGA AGGACCGGTT GCCGTACAGG GCAAGCCTGC 351 CGCCCAAGCG CGCGAAGAGG CTCTGAAACT GCTGGAAAAA GTCGGCTTGG 401 GCGACAAAGT GGATTTGTAT CCCTACCAGC TTTCCGGCGG TCAGCAGCAG 451 CGCGTCGGCA TTGCCCGCGC ATTGGCGATT CAGCCTGAAC TGATGCTGTT 501 TGACGAACCG ACTTCCGCGC TCGATCCTGA ATTGGTGCAA GATGTTTTGG 551 ATACCATGAA GGAATTGGCG CAAGAAGGCT GGACCATGGT TGTCGTTACG 601 CATGAAATCA AGTTCGCCTT AGAAGTGGCA ACCACCGTCG TCGTGATGGA 651 CGGCGGCGTT ATTGTCGAAC AAGGCAGCCC GCAAGATTTG TTCGACCACC 701 CCAAACACGA ACGGACGCGG AGATTTTTAA GCCAAATCCA ATCTACCAAG 751 ATTTGA This corresponds to the amino acid sequence <SEQ ID 454; ORF 122-1>: ml22-l.pep 1 MIKIRΝIHKT FGEΝTILRGI DLDVCKGQVV VILGPSGSGK TTFLRCLΝAL 51 EMPEDGQIEF DΝERPLKIDF SKKPSKHDIL ALRRKSGMVF QQYΝLFPHKT 101 ALEΝVMEGPV AVQGKPAAQA REEALKLLEK VGLGDKVDLY PYQLSGGQQQ 151 RVGIARALAI QPELMLFDEP TSALDPELVQ DVLDTMKELA QEGWTMVVVT 201 HEIKFALEVA TTVVVMDGGV IVEQGΞPQDL FDHPKHERTR RFLΞQIQSTK 251 I* l22-l/gl22-l 94.8% identity in 251 aa overlap 10 20 30 40 50 60 ml22-l.pep MIKIRΝIHKTFGEΝTILRGIDLDVCKGQVVVILGPSGSGKTTFLRCLΝALEMPEDGQIEF 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 gl22 - l MIKIRΝIHKTFGEΝTILRGIDLDVGKGQ VVILGPSGSGKTTFLRCLΝALEMPEDGQIEF 10 20 30 40 50 60 70 80 90 100 110 120 ml22-l.pep DNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTALENVMEGPVAVQGKPAAQA II I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I gl22-l DNARPLRIDFSKKTSKHDILALRRKSGMVFQQYNLFPHKTVLENVMEGPVAVQGKPAAQA 70 80 90 100 110 120 130 140 150 160 170 180 ml22-l.pep REEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAIQPELMLFDEPTSALDPELVQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl22-l REEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAIQPELMLFDEPTSALDPELVQ 130 140 150 160 170 180 190 200 210 220 230 240 ml22-l.pep DVLDTMKELAQEGWTMVVVTHEIKFALEVATTVVVMDGGVIVEQGSPQDLFDHPKHERTR I I I I : I I I I I :| I I I I I I I I I I I I I : I I I I I :l I I I I I I I I I I I I I I ::| I I I llllll gl22-l DVLDAMKELAREGWTMVVVTHEIKFTLEVATNVVVMDGGVIVEQGSPKELFDHLKHERTR 190 200 210 220 230 240 ; 250 ml22 -1.pep RFLSQIQSTKIX 1 : 111 g!22 -1 RFLSQIQSAKIX : 250 The following partial DNA sequence was identified in N. menin< al22 -1. seq 1 ATGATTAAAA TCCGCAATAT CCATAAGACC TTCGGCAAAA ATACCATTTT 51 GCGCGGCATC AATTTGGATG TGTGCAAAGG GCAGGTGGTC GTCATCCTCG 101 GGCCTTCCGG CTCAGGCAAA ACGACGTTTC TGCGATGCCT AAACGCGTTG 151 GAAATGCCCG AAGACGGACA AATCGAGTTC GACAACGAGC GACCGCTGAA 201 AATCGATTTT TCTAAAAAAC CAAGCAAACA CGATATTTTG GCACTGCGCC 251 GCAAATCAGG CATGGTGTTT CAACAATACA ACCTCTTTCC GCACAAAACC 301 GCCTTGGAAA ACGTGATGGA AGGACCGGTT GCCGTACAGG GCAAGCCTGC 351 CGCCCAAGCG CGCGAAGAGG CTCTGAAACT GCTGGAAAAA GTCGGCTTGG 401 GCGACAAAGT GGATTTGTAT CCCTACCAGC TTTCCGGCGG TCAGCAGCAG 451 CGCGTCGGCA TTGCCCGAGC ATTGGCGATT CAGCCCGAGC TGATGTTGTT 501 TGACGAACCC ACTTCCGCGC TTGACCCCGA GTTGGTGCAA GACGTGTTGA 551 ACGCCATGAA GGAATTGGCG CGGGAAGGTT GGACGATGGT CGTCGTTACC 601 CACGAAATCA AGTTCGCGCT GGAAGTTGCC ACGACCGTTG TCGTGATGGA 651 CGGCGGCGTT ATCGTAGAGC AGGGCAGCCC GAAAGAGTTG TTCGACCACC 701 CCAAACACGA ACGGACGCGG AGATTTTTAA GCCAAATCCA ATCTACCAAG 751 ATTTGA This corresponds to the amino acid sequence <SEQ ID 456; ORF 122-l.a>: al22-l.pep 1 MIKIRΝIHKT FGKΝTILRGI ΝLDVCKGQVV VILGPSGSGK TTFLRCLΝAL 51 EMPEDGQIEF DΝERPLKIDF SKKPSKHDIL ALRRKSGMVF QQYΝLFPHKT 101 ALEΝVMEGPV AVQGKPAAQA REEALKLLEK VGLGDKVDLY PYQLSGGQQQ 151 RVGIARALAI QPELMLFDEP TSALDPELVQ DVLΝAMKELA REGWTMVVVT 201 HEIKFALEVA TTVVVMDGGV IVEQGSPKEL FDHPKHERTR RFLSQIQSTK 251 I* a!22-l/ml22-l 97.2% identity in 251 aa overlap 10 20 30 40 50 60 al22-l.pep MIKIRΝIHKTFGKΝTILRGIΝLDVCKGQVVVILGPSGSGKTTFLRCLΝALEMPEDGQIEF I I I I I I I I I I I I : I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml22-l MIKIRΝIHKTFGEΝTILRGIDLDVCKGQVVVILGPSGSGKTTFLRCLΝALEMPEDGQIEF 10 20 30 40 50 60 70 80 90 100 110 120 al22-1. pep DΝERPLKIDFSKKPSKHDILALRRKSGMVFQQYΝLFPHKTALEΝVMEGPVAVQGKPAAQA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml22-l DΝERPLKIDFΞKKPSKHDILALRRKSGMVFQQYΝLFPHKTALEΝVMEGPVAVQGKPAAQA 70 80 90 100 110 120 130 140 150 160 170 180 a122-1. pep REEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAIQPELMLFDEPTSALDPELVQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml22-l REEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAIQPELMLFDEPTSALDPELVQ 130 140 150 160 170 180 190 200 210 220 230 240 al22-l . pep DVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDGGVIVEQGSPKELFDHPKHERTR I I I :: I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ::| I I I I I I I I I I ml22-1 DVLDTMKELAQEGWTMVVVTHEIKFALEVATTVVVMDGGVIVEQGSPQDLFDHPKHERTR 190 200 210 220 230 240 250 al22-l.pep RFLΞQIQSTKIX I I I I I I I I I I I I ml22-l RFLSQIQSTKIX 250 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 457>: gl25. seq 1 ATGTCGGGCA ATGCCTCCTC TCCTTCATCT TCCGCCGCCA TCGGGCTGGT 51 TTGGTTCGGC GCGGCGGTAT CGATTGCCGA AATCAGCACG GGTACGCTGC 101 TCGCCCCCTT GGGCTGGCAG CGCGGTCTGG CGGCCCTGCT TTTGGGTCAT 151 GCCGTCGGCG GCGCGCTGTT TTTTGCGGCG GCGTATATCG GCGCACTGAC 201 CGGACGCAGC TCGATGGAAA GTGTGCGCCT GTCGTTCGGC AAATGCGGTT 251 CAGTGCTGTT TTCCGTGGCG AATATGCTGC AACTGGCCGG CTGGACGGCG 301 GTGATGATTT ACGTCGGCGC AacggTCAGC TCCGCTTTGG GCAAAGTGTT 351 GTGGGACggc gaATCCTTTG TCTGGTGGGC ATTGGCAAAC GGCGCACTGA 401 TCGTGCTGTG GCTGGTTTTC GGCGCACGCA GAACGGGCGG GCTGAAAACC 451 GTTTCGATGC TGCTGATGCT GCTTGCCGTG TTGTGGTTGA GCGTCGAAGT 501 GTTCGCTTCG TCCGGCACAA ACGCCGCGCC CGCCGTTTCA GACGGCATGA 551 CCTTCGGAAC GGCAGTCGAA CTGTCCGCCG TCATGCCGCT TTCCTGGCTG 601 CCGCTGGCCG CCGACTACAC GCGCCAAGCA CGCCGCCCGT TTGCGGCAAC 651 CCTGACGGCA ACGCTCGCCT ATACGCTGAC GGGCTGCTGG ATGTATGCCT 701 TGGGTTTGGC GGCGGCTCTG TTTACCGGAG AAACCGACGT GGCGAAAATC 751 CTGTTGGGCG CGGGCTTGGG CATAACGGGC ATTCTGGCAG TCGTCCTCTC 801 CACCGTTACC ACAACGTTTC TCGATACCTA TTCCGCCGGC GCGAGTGCGA 851 ACAACATTTC CGCGCGTTTT GCGGAAATAC CCGTCGCTGT CGGCGTTACC 901 CTGatccgca ccgtgcttgc cgtcatgctg cccgttaccg aatataaaaa 951 cttcctgctg cttatccgct cggtatttgg gccgatggcg ggtggttttg 1001 attgccgaCT TTTttgtctt AAAACGGCGT GA This corresponds to the amino acid sequence <SEQ ID 458; ORF 125.ng>: The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 459>: ml25. seq 1 ATGTCGGGCA ATGCCTCCTC TCCTTCATCT TCCTCCGCCA TCGGGCTGAT 51 TTGGTTCGGC GCGGCGGTAT CGATTGCCGA AATCAGCACG GGTACGCTGC 101 TTGCGCCTTT GGGCTGGCAG CGCGGTCTGG CGGCTCTACT TTTGGGTCAT 151 GCCGTCGGCG GCGCGCTGTT TTTTGCGGCG GCGTATATCG GCGCACTGAC 201 CGGACGCAGC TCGATGGAAA GCGTGCGCCT GTCGTTCGGC AAACGCGGTT 251 CAGTGCTGTT TTCCGTGGCG AATATGCTGC AACTGGCCGG CTGGACGGCG 301 GTGATGATTT ACGCCGGCGC AACGGTCAGC TCCGCTTTGG GCAAAGTGTT 351 GTGGGACGGC GAATCTTTTG TCTGGTGGGC ATTGGCAAAC GGCGCGCTGA 401 TTGTGCTGTG GCTGGTTTTC GGCGCACGCA AAACAGGCGG GCTGAAAACC 451 GTTTCGATGC TGCTGATGCT GTTGGCGGTT CTGTGGCTGA GTGCCGAAGT 501 CTTTTCCACG GCAGGCAGCA CCGCCGCACA GGTTTCAGAC GGCATGAGTT 551 TCGGAACGGC AGTCGAGCTG TCCGCCGTGA TGCCGCTTTC CTGGCTGCCG 601 CTTGCCGCCG ACTACACGCG CCACGCGCGC CGCCCGTTTG CGGCAACCCT 651 GACGGCAACG CTCGCCTACA CGCTGACCGG CTGCTGGATG TATGCCTTGG 701 GTTTGGCAGC GGCGTTGTTC ACCGGAGAAA CCGACGTGGC AAAAATCCTG 751 CTGGGCGCAr GTTTGgGTGC GGCAGGCATT TTGGCGGTCG TCCTCTCCAC 801 CGTTACCACA ACGTTTCTCG ATGCCTATTC CGCCGGCGCG AGTGCGAACA 851 ACATTTCCGC GCGTTTTGCG GAAACACCCG TCGCTGTCrG CGTTACCCTG 901 ATCGGCACGG TACTTGCCGT CATGCTGCCC GTTACCGAAT ATGAAAACTT 951 CCTGCTGCTT ATCGGCTCGG TATTTGCGCC GATGGCGGgC GGTTTTGATT 1001 GCCGACTTTT TCGTCTTGAA ACGGCGTGA This corresponds to the amino acid sequence <SEQ ID 460; ORF 125>: ml25.pep 1 MSGNASSPSS SSAIGLIWFG AAVSIAEIST GTLLAPLGWQ RGLAALLLGH 51 AVGGALFFAA AYIGALTGRS SMESVRLSFG KRGSVLFSVA NMLQLAGWTA 101 VMIYAGATVS SALGKVLWDG ESFVWWALAN GALIVLWLVF GARKTGGLKT 151 VSMLLMLLAV LWLSAEVFST AGSTAAQVSD GMSFGTAVEL SAVMPLSWLP 201 LAADYTRHAR RPFAATLTAT LAYTLTGCWM YALGLAAALF TGETDVAKIL 251 LGAXLGAAGI LAWLSTVTT TFLDAYSAGA SANNISARFA ETPVAVXVTL 301 IGTVLAVMLP VTEYENFLLL IGSVFAPMAG GFDCRLFRLE TA* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 125 shows 92.1% identity over a 343 aa overlap with a predicted ORF (ORF 125.ng) from N. gonorrhoeae: ml25/gl25 10 20 30 40 50 60 ml25.pep MSGΝASSPSSSSAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLAALLLGHAVGGALFFAA 11111111111 = 111 I = 11111 E I ! ! I i 1111 E 111 E j ! I E [ t E 1111111111111111 gl25 MSGΝASSPSSSAAIGLVWFGAAVSIAEISTGTLLAPLGWQRGLAALLLGHAVGGALFFAA 10 20 30 40 50 60 70 80 90 100 110 120 ml25.pep AYIGALTGRSSMESVRLSFGKRGSVLFSVAΝMLQLAGWTAVMIYAGATVSSALGKVLWDG gl25 AYIGALTGRSSMESVRLSFGKCGSVLFSVAΝMLQLAGWTAVMIYVGATVSSALGKVLWDG 70 80 90 100 110 120 130 140 150 160 170 179 ml25.pep ESFVWWALAΝGALIVLWLVFGARKTGGLKTVSMLLMLLAVLWLSAEVFSTAGSTAAQ-VS I I gl25 ESFVWWALAΝGALIVLWLVFGARRTGGLKTVSMLLMLLAVLWLSVEVFASSGTΝAAPAVS 130 140 150 160 170 180 180 190 200 210 220 230 239 ml25.pep DGMSFGTAVELSAVMPLSWLPLAADYTRHARRPFAATLTATLAYTLTGCWMYALGLAAAL gl25 DGMTFGTAVELSAVMPLSWLPLAADYTRQARRPFAATLTATLAYTLTGCWMYALGLAAAL 190 200 210 220 230 240 240 250 260 270 280 290 299 ml 25 . pep FTGETDVAKILLGAXLGAAGILAWLSTVTTTFLDAYSAGASAΝΝISARFAETPVAVXVT I I I I M I I M I I I I I I = 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 h 1 1 1 1 1 1 1 1 1 1 1 1 1 I I I M M I I gl25 FTGETDVAKILLGAGLGITGILAWLSTVTTTFLDTYSAGASAΝΝISARFAEIPVAVGVT 250 260 270 280 290 300 300 310 320 330 340 ml25.pep LIGTVLAVMLPVTEYEΝFLLLIGSVFAPMAGGFDCRLFRLETAX I I M I M M M I I h l l M M h i l l gl25 LIRTVLAVMLPVTEYKΝFLLLIRSVFGPMAGGFDCRLFCLKTAX 310 320 330 340 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 461>: al25. seq 1 ATGTCGGGCA ATGCCTCCTC TCCTTCATCT TCCGCCGCCA TCGGGCTGAT 51 TTGGTTCGGC GCGGCGGTAT CGATTGCCGA AATCAGCACG GGTACACTGC 101 TTGCGCCTTT GGGCTGGCAG CGCGGTCTGG CGGCTCTGCT TTTGGGTCAT 151 GCCGTCGGCG GCGCGCTGTT TTTTGCGGCG GCGTATATCG GCGCACTGAC 201 CGGACGCAGC TCGATGGAAA GCGTGCGCCT GTCGTTCGGC AAACGCGGTT 251 CAGTGCTGTT TTCCGTGGCG AATATGCTGC AACTGGCCGG CTGGACGGCG 301 GTGATGATTT ACGCCGGCGC AACGGTCAGC TCCGCTTTGG GCAAAGTGTT 351 GTGGGACGGC GAATCTTTTG TCTGGTGGGC ATTGGCAAAC GGCGCGCTGA 401 TTGTGCTGTG GCTGGTTTTC GGCGCACGCA AAACAGGCGG GCTGAAAACC 451 GTTTCGATGC TGCTGATGCT GTTGGCGGTT CTGTGGCTGA GTGCCGAAGT 501 CTTTTCCACG GCAGGCAGCA CCGCCGCACA GGTTTCAGAC GGCATGAGTT 551 TCGGAACGGC AGTCGAGCTG TCCGCCGTGA TGCCGCTTTC TTGGCTGCCG 601 CTGGCCGCCG ACTACACGCG CCACGCGCGC CGCCCGTTTG CGGCAACCCT 651 GACGGCAACG CTCGCCTACA CGCTGACCGG CTGCTGGATG TATGCCTTGG 701 GTTTGGCAGC GGCGTTGTTC ACCGGAGAAA CCGACGTGGC AAAAATCCTG 751 CTGGGCGCAG GTTTGGGTGC GGCAGGCATT TTGGCGGTCG TCCTGTCGAC 801 CGTTACCACC ACTTTTCTCG ATGCCTACTC CGCCGGCGTA AGTGCCAACA 851 ATATTTCCGC CAAACTTTCG GAAATACCCA TCGCCGTTGC CGTCGCCGTT 901 GTCGGCACAC TGCTTGCCGT CCTCCTGCCC GTTACCGAAT ATGAAAACTT 951 CCTGCTGCTT ATCGGCTCGG TATTTGCGCC GATGGCG . GC GGTTTTGATT 1001 GCCGACTTTT TCGTCTTGAA ACGGCGTGA This corresponds to the amino acid sequence <SEQ ID 462; ORF 125. a>: al25.pep 1 MSGNASSPSS SAAIGLIWFG AAVSIAEIST GTLLAPLGWQ RGLAALLLGH 51 AVGGALFFAA AYIGALTGRS SMESVRLSFG KRGSVLFSVA NMLQLAGWTA 101 VMIYAGATVS SALGKVLWDG ESFVWWALAN GALIVLWLVF GARKTGGLKT 151 VSMLLMLLAV LWLSAEVFST AGSTAAQVSD GMSFGTAVEL SAVMPLSWLP 201 LAADYTRHAR RPFAATLTAT LAYTLTGCWM YALGLAAALF TGETDVAKIL 251 LGAGLGAAGI LAVVLSTVTT TFLDAYSAGV SANNISAKLS EIPIAVAVAV 301 VGTLLAVLLP VTEYENFLLL IGSVFAPMAX GFDCRLFRLE TA* ml25/al25 95.6% identity in 342 aa overlap 10 20 30 40 50 60 ml25. pep MSGNASSPSSSSAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLAALLLGHAVGGALFFAA I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I al25 MSGNASSPSSSAAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLAALLLGHAVGGALFFAA 10 20 30 40 50 60 70 80 90 100 110 120 ml25. pep AYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVMIYAGATVSSALGKVLWDG I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I al25 AYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVMIYAGATVSSALGKVLWDG 70 80 90 100 110 120 130 140 150 160 170 180 ml25. pep ESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAVLWLSAEVFSTAGSTAAQVSD I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al25 ESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAVLWLSAEVFSTAGSTAAQVSD 130 140 150 160 170 180 190 200 210 220 230 240 ml25. pep GMSFGTAVELSAVMPLSWLPLAADYTRHARRPFAATLTATLAYTLTGCWMYALGLAAALF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I al25 GMSFGTAVELSAVMPLSWLPLAADYTRHARRPFAATLTATLAYTLTGCWMYALGLAAALF 190 200 210 220 230 240 250 260 270 280 290 300 ml25. pep TGETDVAKILLGAXLGAAGILAVVLSTVTTTFLDAYSAGASANNISARFAETPVAVXVTL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I ::: I I: I I I : : al25 TGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAYSAGVSANNISAKLSEIPIAVAVAV 250 260 270 280 290 300 310 320 330 340 ml25.pep IGTVLAVMLPVTEYENFLLLIGSVFAPMAGGFDCRLFRLETAX : I I : I I I : I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I al25 VGTLLAVLLPVTEYENFLLLIGSVFAPMAXGFDCRLFRLETAX 310 320 330 340 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 463>: gl26. seq 1 AtgccgtcTG AAaccCcaaa ggcACGCCGC CGGCTTTCAG ACGGCATCGC 51 GTCCGACAAC CATACCAAAG AATCCATCAT GCTCACCctg tacggcGAAA 101 CTTTCCCTTC GCGGCTGCTg ctcggcacgG cggcctacCC GACCCCTGAA 151 ATCCTCAAAC AATCCGTCCG AACCGCCCGG CCCGCGATGA ttaccGTCTC 201 GCTGCGCCGC ACGGGATGCG GCGGCGAGGC GCACGGTCAG GGGTTTTGGT 251 CGCTGCTTCA AGAAACCGGC GTTCCCGTCC TGCCGAACAC GGCAGGCTGC 301 CAAAGCGTGC AGGAAGCGGT AACGACGGCG CAAATGGCGC GCGAAGTGTT 351 TGAAACCGAT TGGATAAAAT TGGAACTCAT CGGCGACGAC GACACCTTGC 401 AGCCGGACGT GTTCCAACTC GTCGAAGCGG CGGAAATCCT GATTAAAGAC 451 GGCTTCAAAG TGCTGCCTTA TTGCACCGAA GACCTGATTG CCTGCCGCCG 501 CCTGCTCGAT GCGGGCTGTC AGGCGTTGAT GCCGTGGGCG GCTCCCATCG 551 GCACGGGTTT GGGGGCGGTT CACGCCTATG CGCTCAAAAT CCTGCGCGAA 601 CGCCTGCCCG ACACGCCGCT GATTATCGAC GCGGGCTTGG GTTTGCCTTC 651 CCAAGCGGCA CAAGTGATGG AATGGGGTTT TGACGGCGTA TTGTTAAACA 701 CCGCCGTTTC CCGCAGCGGC GACCCCGTCA ACATGGCGCG CGCCTTCGCA 751 CTCGCCGTCG AATCCGGACG GCTGGCATTT GAAGCCGGGC CGGTCGAAGC 801 GCGAACCAAA GCCCAAGCCA GCACGCCGAC AGTCGGACAA CCGTTTTGGC 851 ATTCGGCGGA ATATTGA This corresponds to the amino acid sequence <SEQ ID 464; ORF 126.ng>: gl26.pep 1 MPSETPKARR RLSDGIASDΝ HTKESIMLTL YGETFPSRLL LGTAAYPTPE 51 ILKQSVRTAR PAMITVSLRR TGCGGEAHGQ GFWSLLQETG VPVLPΝTAGC 101 QSVQEAVTTA QMAREVFETD WIKLELIGDD DTLQPDVFQL VEAAEILIKD 151 GFKVLPYCTE DLIACRRLLD AGCQALMPWA APIGTGLGAV HAYALKILRE 201 RLPDTPLIID AGLGLPSQAA QVMEWGFDGV LLΝTAVSRSG DPVΝMARAFA 251 LAVESGRLAF EAGPVEARTK AQASTPTVGQ PFWHSAEY* The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 465>: ml26.seq (partial) 1 .. CACTATACAA AGGAACCCAT TATGCTCACC CTATACGGCG AAACTTTCCC 51 CTCGCGGCTG CTGCTCGGCA CGGCTGCCTA CCCGACCCCC GAAATCCTCA 101 AACAATCCAT CCAAACCGCC CAGCCTGCGA TGATTACCGT CTCGCTGCGC 151 CGCGCGGGAA GCGGCGGCGA GGCGCACGGT CAGGGGTTTT GGTCGCTGCT 201 TCAAGAAACC GGCGTTCCCG TCCTGCCGAA CACGGCAGGC TGCCAAAGCG 251 TGCAGGAAGC GGTAACGACG GCGCAAATGG CGCGCGAAGT GTTTGAAACC 301 GATTGGATAA AATTGGAACT CATCGGAGAT GACGACACCT TGCAGCCGGA 351 TGTGTTCCAG CTTGTCGAAG CGGCGGAAAT CCTGATTAAA GACGGCTTCA 401 AAGTGCTGCC TTATTGCACC GAAGACCTGA TTGCCTGCCG CCGCCTGCTC 451 GACGCGGGCT GTCAGGCGTT GATGCCGTGG GCGGCCCCGA TCGGCACGGG 501 TTTGGGCGCG GTTCACGCCT ACGCGTTGAA CGTCCTGCGC GAACGCCTGC 551 CCGACACGCC GCTGATTATC GACGCGGGCT TGGGTTTGCC CTCACAGGCG 601 GCACAAGTGA TGGAATGGGG CTTTGACGGC GTGCTTTTGA ATACTGCCGT 651 TTCCCGCAGC GGCGATCCGG TCAATATGGC ACGCGCCTTC GCACTCGCCG 701 TCGAATCCGG ACGGCTGGCA TTTGAAGCCG GACCGGTCGA AGCACGCGAC 751 AAAGCGCAAG CCAGCACGCC GACAGTCGGA CAACCGTTTT GGCATTCGGC 801 GGAATATTGA This corresponds to the amino acid sequence <SEQ ID 466; ORF 126>: ml26.pep (partial) 1 ..HYTKEPIMLT LYGETFPSRL LLGTAAYPTP EILKQSIQTA QPAMITVSLR 51 RAGSGGEAHG QGFWSLLQET GVPVLPΝTAG CQSVQEAVTT AQMAREVFET 101 DWIKLELIGD DDTLQPDVFQ LVEAAEILIK DGFKVLPYCT EDLIACRRLL 151 DAGCQALMPW AAPIGTGLGA VHAYALNVLR ERLPDTPLII DAGLGLPSQA 201 AQVMEWGFDG VLLNTAVSRS GDPVNMARAF ALAVESGRLA FEAGPVEARD 251 KAQASTPTVG QPFWHSAEY* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 126 shows 95.9% identity over a 269 aa overlap with a predicted ORF (ORF 126.ng) from N gonorrhoeae: ml26/gl26 10 20 30 40 ml26.pep HYTKEPIMLTLYGETFPSRLLLGTAAYPTPEILKQSIQTAQ -III 1111111111 i 111111111 I 11 II 11 M I :: 11 : g126 MPSETPKARRRLSDGIASDΝHTKESIMLTLYGETFPSRLLLGTAAYPTPEILKQSVRTAR 10 20 30 40 50 60 50 60 70 80 90 100 ml26. pep PAMITVSLRRAGSGGEAHGQGFWSLLQETGVPVLPΝTAGCQSVQEAVTTAQMAREVFETD 1 1 1 1 1 1 1 1 1 h i M M M M M M M M M M M M I M M I M I M M M I M M M I gl26 PAMITVSLRRTGCGGEAHGQGFWSLLQETGVPVLPΝTAGCQSVQEAVTTAQMAREVFETD 70 80 90 100 110 120 110 120 130 140 150 160 ml26.pep WIKLELIGDDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWA II 1111 II 11111 II 11 ! 111 II 11 II 11111 II 111 II 1111111 II I II I M 111111 g126 WIKLELIGDDDTLQPDVFQLVEAAEI IKDGFKVLPYCTEDLIACRRLLDAGCQALMPWA 130 140 150 160 170 180 170 180 190 200 210 220 ml26. pep APIGTGLGAVHAYALΝVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLΝTAVSRSG 11111 II 1111111 = 1111111111111111111 M 11111111 M 111111111 M I gl26 APIGTGLGAVHAYALKILRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLΝTAVSRSG 190 200 210 220 230 240 230 240 250 260 270 ml26. pep DPVΝMARAFALAVESGRLAFEAGPVEARDKAQASTPTVGQPFWHSAEYX 11111111 i 1111111 ! 11111111 i 11 Ii 1111111 M j 1111111 i gl26 DPVΝMARAFALAVESGRLAFEAGPVEARTKAQASTPTVGQPFWHSAEYX 250 260 270 280 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 467>: al26. seq 1 TTGTTAATCC ACTATACAAA GGAACCCATT ATGCTCACCC TGTACAGCGA 51 AACTTTCCCT TCGCGGCTGC TGCTCGGCAC AGCCGCCTAC CCGACCCCTG 101 AAATCCTCAA ACAATCCGTC CGAACCGCCC GGCCCGCGAT GATTACCGTC 151 TCGCTGCGCC GCGCGGGATG CGGCGGCGAG GCGCACGGTC AGGGGTTTTG 201 GTCGCTGCTT CAAGAAACCG GCGTTCCCGT CCTGCCGAAC ACGGCAGGCT 251 GCCAAAGCGT GCAGGAAGCG GTAACGACGG CGCAAATGGC GCGCGAAGTG 301 TTTGAAACCG ATTGGATTAA ACTCGAACTC ATCGGCGACG ACGACACCTT 351 GCAGCCGGAT GTGTTCCAAC TTGTCGAAGC GGCGGAAATC CTGATTAAAG 401 ACGGCTTCAA AGTGCTGCCT TATTGCACCG AAGACCTGAT TGCCTGCCGC 451 CGCCTGCTCG ACGCGGGCTG TCAGGCGTTG ATGCCGTGGG CGGCCCCGAT 501 CGGCACGGGT TTGGGCGCGG TTCACGCCTA CGCGTTGAAC GTCCTGCGCG 551 AACGCCTGCC CGACACGCCG CTGATTATCG ACGCGGGCTT GGGTTTGCCC 601 TCACAGGCGG CACAAGTGAT GGAATGGGGC TTTGACGGCG TGCTTTTGAA 651 TACTGCCGTT TCCCGCAGCG GCGATCCGGT CAATATGGCA CGCGCCTTCG 701 CACTCGCCGT CGAATCCGGA CGGCTGGCAT TTGAAGCCGG ACCGGTCGAA 751 GCACGCGACA AAGCGCAAGC CAGCACGCCG ACAGTCGGAC AACCGTTTTG 801 GCATTCGGCG GAATATTGA This corresponds to the amino acid sequence <SEQ ID 468; ORF 126.a>: al26.pep 1 LLIHYTKEPI MLTLYSETFP SRLLLGTAAY PTPEILKQSV RTARPAMITV 51 SLRRAGCGGE AHGQGFWSLL QETGVPVLPN TAGCQSVQEA VTTAQMAREV 101 FETDWIKLEL IGDDDTLQPD VFQLVEAAEI LIKDGFKVLP YCTEDLIACR 151 RLLDAGCQAL MPWAAPIGTG LGAVHAYALN VLRERLPDTP LIIDAGLGLP 201 SQAAQVMEWG FDGVLLNTAV SRSGDPVNMA RAFALAVESG RLAFEAGPVE 251 ARDKAQASTP TVGQPFWHSA EY* ml26/al26 98.1% identity in 269 aa overlap 10 20 30 40 50 ml26.pep HYTKEPIMLTLYGETFPSRLLLGTAAYPTPEILKQSIQTAQPAMITVSLRRAGSGGE I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I :: I I : I I I I I I I I I I I I III al26 LLIHYTKEPIMLTLYSETFPSRLLLGTAAYPTPEILKQSVRTARPAMITVSLRRAGCGGE 10 20 30 40 50 60 60 70 80 90 100 110 ml26.pep AHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIGDDDTLQPD I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al26 AHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIGDDDTLQPD 70 80 90 100 110 120 120 130 140 150 160 170 ml26.pep VFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTGLGAVHAYALN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al26 VFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTGLGAVHAYALN 130 140 150 160 170 180 180 190 200 210 220 230 ml26.pep VLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVNMARAFALAVESG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I al26 VLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVNMARAFALAVESG 190 200 210 220 230 240 240 250 260 270 ml26.pep RLAFEAGPVEARDKAQASTPTVGQPFWHSAEYX I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I al26 RLAFEAGPVEARDKAQASTPTVGQPFWHSAEYX 250 260 270 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 469>: gl26-l.seq 1 ATGCTCACCC TGTACGGCGA AACTTTCCCT TCGCGGCTGC TGCTCGGCAC 51 GGCCGCCTAC CCGACCCCTG AAATCCTCAA ACAATCCGTC CGAACCGCCC 101 GGCCCGCGAT GATTACCGTC TCGCTGCGCC GCACGGGATG CGGCGGCGAG 151 GCGCACGGTC AGGGGTTTTG GTCGCTGCTT CAAGAAACCG GCGTTCCCGT 201 CCTGCCGAAC ACGGCAGGCT GCCAAAGCGT GCAGGAAGCG GTAACGACGG 251 CGCAAATGGC GCGCGAAGTG TTTGAAACCG ATTGGATAAA ATTGGAACTC 301 ATCGGCGACG ACGACACCTT GCAGCCGGAC GTGTTCCAAC TCGTCGAAGC 351 GGCGGAAATC CTGATTAAAG ACGGCTTCAA AGTGCTGCCT TATTGCACCG 401 AAGACCTGAT TGCCTGCCGC CGCCTGCTCG ATGCGGGCTG TCAGGCGTTG 451 ATGCCGTGGG CGGCTCCCAT CGGCACGGGT TTGGGGGCGG TTCACGCCTA 501 TGCGCTCAAA ATCCTGCGCG AACGCCTGCC CGACACGCCG CTGATTATCG 551 ACGCGGGCTT GGGTTTGCCT TCCCAAGCGG CACAAGTGAT GGAATGGGGT 601 TTTGACGGCG TATTGTTAAA CACCGCCGTT TCCCGCAGCG GCGACCCCGT 651 CAACATGGCG CGCGCCTTCG CACTCGCCGT CGAATCCGGA CGGCTGGCAT 701 TTGAAGCCGG GCCGGTCGAA GCGCGAACCA AAGCCCAAGC CAGCACGCCG 751 ACAGTCGGAC AACCGTTTTG GCATTCGGCG GAATATTGA This corresponds to the amino acid sequence <SEQ ID 470; ORF 126-l.ng>: gl26-l.pep 1 MLTLYGETFP SRLLLGTAAY PTPEILKQSV RTARPAMITV SLRRTGCGGE 51 AHGQGFWSLL QETGVPVLPN TAGCQSVQEA VTTAQMAREV FETDWIKLEL 101 IGDDDTLQPD VFQLVEAAEI LIKDGFKVLP YCTEDLIACR RLLDAGCQAL 151 MPWAAPIGTG LGAVHAYALK ILRERLPDTP LIIDAGLGLP SQAAQVMEWG 201 FDGVLLNTAV SRSGDPVNMA RAFALAVESG RLAFEAGPVE ARTKAQASTP 251 TVGQPFWHSA EY* The following partial DNA sequence was identified in N meningitidis <SEQ ID 471>: ml26-l.seq 1 ATGCTCACCC TATACGGCGA AACTTTCCCC TCGCGGCTGC TGCTCGGCAC 51 GGCTGCCTAC CCGACCCCCG AAATCCTCAA ACAATCCATC CAAACCGCCC 101 AGCCTGCGAT GATTACCGTC TCGCTGCGCC GCGCGGGAAG CGGCGGCGAG 151 GCGCACGGTC AGGGGTTTTG GTCGCTGCTT CAAGAAACCG GCGTTCCCGT 201 CCTGCCGAAC ACGGCAGGCT GCCAAAGCGT GCAGGAAGCG GTAACGACGG 251 CGCAAATGGC GCGCGAAGTG TTTGAAACCG ATTGGATAAA ATTGGAACTC 301 ATCGGAGATG ACGACACCTT GCAGCCGGAT GTGTTCCAGC TTGTCGAAGC 351 GGCGGAAATC CTGATTAAAG ACGGCTTCAA AGTGCTGCCT TATTGCACCG 401 AAGACCTGAT TGCCTGCCGC CGCCTGCTCG ACGCGGGCTG TCAGGCGTTG 451 ATGCCGTGGG CGGCCCCGAT CGGCACGGGT TTGGGCGCGG TTCACGCCTA 501 CGCGTTGAAC GTCCTGCGCG AACGCCTGCC CGACACGCCG CTGATTATCG 551 ACGCGGGCTT GGGTTTGCCC TCACAGGCGG CACAAGTGAT GGAATGGGGC 601 TTTGACGGCG TGCTTTTGAA TACTGCCGTT TCCCGCAGCG GCGATCCGGT 651 CAATATGGCA CGCGCCTTCG CACTCGCCGT CGAATCCGGA CGGCTGGCAT 701 TTGAAGCCGG ACCGGTCGAA GCACGCGACA AAGCGCAAGC CAGCACGCCG 751 ACAGTCGGAC AACCGTTTTG GCATTCGGCG GAATATTGA This corresponds to the amino acid sequence <SEQ ID 5; ORF 126-1>: ml26-l.pep 1 MLTLYGETFP SRLLLGTAAY PTPEILKQSI QTAQPAMITV SLRRAGΞGGE 51 AHGQGFWSLL QETGVPVLPN TAGCQSVQEA VTTAQMAREV FETDWIKLEL 101 IGDDDTLQPD VFQLVEAAEI LIKDGFKVLP YCTEDLIACR RLLDAGCQAL 151 MPWAAPIGTG LGAVHAYALN VLRERLPDTP LIIDAGLGLP SQAAQVMEWG 201 FDGVLLNTAV SRSGDPVNMA RAFALAVESG RLAFEAGPVE ARDKAQASTP 251 TVGQPFWHSA EY* ml26-l/gl26-l 96.9% identity in 262 aa overlap 10 20 30 40 50 60 ml26-l.pep MLTLYGETFPSRLLLGTAAYPTPEILKQSIQTAQPAMITVΞLRRAGSGGEAHGQGFWSLL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I :: I I : I I I I I I I I I I :| I I I I I I I I I I I I I gl26-l MLTLYGETFPSRLLLGTAAYPTPEILKQΞVRTARPAMITVSLRRTGCGGEAHGQGFWΞLL 10 20 30 40 50 60 70 80 90 100 110 120 ml26-l.pep QETGVPVLPNTAGCQΞVQEAVTTAQMAREVFETDWIKLELIGDDDTLQPDVFQLVEAAEI I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I gl26-l QETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIGDDDTLQPDVFQLVEAAEI 70 80 90 100 110 120 130 140 150 160 170 180 ml26-l.pep LIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTGLGAVHAYALNVLRERLPDTP I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I :: I I I I I I I I I gl26-l LIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTGLGAVHAYALKILRERLPDTP 130 140 150 160 170 180 190 200 210 220 230 240 ml26-l.pep LIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVNMARAFALAVESGRLAFEAGPVE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl26-l LIIDAGLGLPΞQAAQVMEWGFDGVLLNTAVSRSGDPVNMARAFALAVESGRLAFEAGPVE 190 200 210 220 230 240 250 260 ml26-l.pep ARDKAQASTPTVGQPFWHSAEYX II I I I I I I I I I I I I I I I I I I I I gl26-l ARTKAQASTPTVGQPFWHSAEYX 250 260 The following partial DNA sequence was identified in N meningitidis <SEQ ID 473>: al26-l.seq 1 ATGCTCACCC TGTACAGCGA AACTTTCCCT TCGCGGCTGC TGCTCGGCAC 51 AGCCGCCTAC CCGACCCCTG AAATCCTCAA ACAATCCGTC CGAACCGCCC 101 GGCCCGCGAT GATTACCGTC TCGCTGCGCC GCGCGGGATG CGGCGGCGAG 151 GCGCACGGTC AGGGGTTTTG GTCGCTGCTT CAAGAAACCG GCGTTCCCGT 201 CCTGCCGAAC ACGGCAGGCT GCCAAAGCGT GCAGGAAGCG GTAACGACGG 251 CGCAAATGGC GCGCGAAGTG TTTGAAACCG ATTGGATTAA ACTCGAACTC 301 ATCGGCGACG ACGACACCTT GCAGCCGGAT GTGTTCCAAC TTGTCGAAGC 351 GGCGGAAATC CTGATTAAAG ACGGCTTCAA AGTGCTGCCT TATTGCACCG 401 AAGACCTGAT TGCCTGCCGC CGCCTGCTCG ACGCGGGCTG TCAGGCGTTG 451 ATGCCGTGGG CGGCCCCGAT CGGCACGGGT TTGGGCGCGG TTCACGCCTA 501 CGCGTTGAAC GTCCTGCGCG AACGCCTGCC CGACACGCCG CTGATTATCG 551 ACGCGGGCTT GGGTTTGCCC TCACAGGCGG CACAAGTGAT GGAATGGGGC 601 TTTGACGGCG TGCTTTTGAA TACTGCCGTT TCCCGCAGCG GCGATCCGGT 651 CAATATGGCA CGCGCCTTCG CACTCGCCGT CGAATCCGGA CGGCTGGCAT 701 TTGAAGCCGG ACCGGTCGAA GCACGCGACA AAGCGCAAGC CAGCACGCCG 751 ACAGTCGGAC AACCGTTTTG GCATTCGGCG GAATATTGA This corresponds to the amino acid sequence <SEQ ID 474; ORF 126-1. a>: al26-l.pep 1 MLTLYSETFP SRLLLGTAAY PTPEILKQSV RTARPAMITV SLRRAGCGGE 51 AHGQGFWSLL QETGVPVLPN TAGCQSVQEA VTTAQMAREV FETDWIKLEL 101 IGDDDTLQPD VFQLVEAAEI LIKDGFKVLP YCTEDLIACR RLLDAGCQAL 151 MPWAAPIGTG LGAVHAYALN VLRERLPDTP LIIDAGLGLP SQAAQVMEWG 201 FDGVLLNTAV SRSGDPVNMA RAFALAVESG RLAFEAGPVE ARDKAQASTP 251 TVGQPFWHSA EY* al26-l/ l26-l 98.1% identity in 262 aa overlap 10 20 30 40 50 60 al26-l.pep MLTLYSETFPSRLLLGTAAYPTPEILKQSVRTARPAMITVSLRRAGCGGEAHGQGFWΞLL I I I I I : I I I I I I I I I I I I I I I I I I I I I I I :: I I : I I I I I I I I I I I I I I I I I I I I I I I I I ml26-l MLTLYGETFPSRLLLGTAAYPTPEILKQSIQTAQPAMITVSLRRAGSGGEAHGQGFWSLL 10 20 30 40 50 60 70 80 90 100 110 120 al26-l.pep QETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIGDDDTLQPDVFQLVEAAEI I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml26-l QETGVPVLPNTAGCQΞVQEAVTTAQMAREVFETDWIKLELIGDDDTLQPDVFQLVEAAEI 70 80 90 100 110 120 130 140 150 160 170 180 al26-l.pep LIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTGLGAVHAYALNVLRERLPDTP I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml26-l LIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTGLGAVHAYALNVLRERLPDTP 130 140 150 160 170 180 190 200 210 220 230 240 al26-l.pep LIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVNMARAFALAVESGRLAFEAGPVE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml26-l LIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRΞGDPVNMARAFALAVESGRLAFEAGPVE 190 200 210 220 230 240 250 260 al26-l .pep ARDKAQASTPTVGQPFWHSAEYX I I I I I I I I I I I I I I I I I I I I I I I ml26-l ARDKAQASTPTVGQPFWHSAEYX 250 260 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 475>: gl27.seq 1 ATGGAAATAT GGAATATGTT GAACACTTGG CCCGATGCCG TCCCGATACG 51 CGCGGAGGCG GCCGAATCCG TGGCGGCGGT CGCGGCTTTG CTGCTGGCGC 101 GCGCCCTTCT GTTGAATATC CACTTCAGAC GGCATCCGGA TTTCGGCATC 151 GAAAGCAAGC GGCGGTTTTT GGTTGCCAGC CGCAATATAA CGCTGCTTTT 201 GGTGCTGTTT TCGCTGGCAT TTATCTGGTC GGCGCAAATT CAAACGCTGG 251 CTTTGTCGAT GTTTGCGGTG GCGGCGGCGG TCGTCGTGGC GACAAAAGAA 301 CTGATTATGT GTCTGTCGGG CAGTATTTTA aggtctGCCA CCCAGCAATA 351 CTCGGTCGGC GACTATATCG AAATCAACGG CCTGCGCGGG CGCGTGGTCG 401 ACATCAATCT GTTGAACACG CTGATGATGC AGGTCGGTCC GAACCCCTTG 451 GTCGGACAGC TTGCGGGAAC CACCGTTTCT TTCCCCAACA GCCTGTTGTT 501 GAGCCACCCC GTGCGCCGCG ACAATATTTT GGGCGACTAT GTCATCCATA 551 CGGTCGAAAT CCCCGTTCCC ATCCATTTGG ATTCGGATGA AGCCGTATGC 601 CGTCTGAAAG CCGTACTCGA GCCCTTGTGC GCGCCCTACA TCCCCGCCAT 651 TCAGCGGTAT TTGGAAAACG TGCAGGCGGA AAAACTGTTT ATCACGCCCG 701 CCGCCAGGCC GCGCGTTACC CGCGTACCGT ACGACGACAA GGCATACCGC 751 ATCATCGTCC GCTTCGCCTC CCCCGTTTCA AAGCGGCTGG AAATCCAACA 801 GGCGGTTATG GACGAATTTT TGCGCGTACA ATACCGCCTG TTAAATCATC 851 CCGCCGgctc cgAAACACTT TAA This corresponds to the amino acid sequence <SEQ ID 476; ORF 127.ng>: gl27.pep 1 MEIWNMLNTW PDAVPIRAEA AESVAAVAAL LLARALLLNI HFRRHPDFGI 51 ESKRRFLVAS RNITLLLVLF SLAFIWSAQI QTLALSMFAV AAAWVATKE 101 LIMCLSGSIL RSATQQYSVG DYIEINGLRG RWDINLLNT LMMQVGPNPL 151 VGQLAGTTVS FPNSLLLSHP VRRDNILGDY VIHTVEIPVP IHLDSDEAVC 201 RLKAVLEPLC APYIPAIQRY LENVQAEKLF ITPAARPRVT RVPYDDKAYR 251 IIVRFASPVS KRLEIQQAVM DEFLRVQYRL LNHPAGSETL * The following partial DNA sequence was identified in N. meningitidis <SEQ ID 477>: ml27.seq 1 ATGGAAATAT GGAATATGTT GGACACTTGG CTCGGTGCCG TCCCGATACG 51 TGCGGAGGCG GTCGAATCCG TGGCGGCGGT TGCGGCTTTG CTGCTGGCGC 101 GCGCCCTTCT GTTGAATATC CACTTCAAAC GGCATCCGGA TTTCGGCATC 151 GAAAGCAAGC GGCGGTTTTT GGTTGCCAGC CGCAATATAA CGCTGCTTTT 201 GGTGCTGTTT TCGCTGGCAT TTATCTGGTC GGCGCAAATC CAAACGCTGG 251 CTTTGTCGAT GTTTGCGGTG GCGGCGGCGG TCGTCGTGGC GACGAAGGAA 301 CTGATTATGT GTCTGTCGGG CAGTATTTTA AGGTCTGCCA CCCAGCAATA 351 CTCGGTCGGC GACTATATCG AAATCAACGG CCTGCGCGGG CGCGTGGTCG 401 ACATCAACCT GTTGAACACG CTGATGATGC AGGTCGGTCC GAACCCCTTG 451 GTCGGACAGC TTGCGGGAAC CACCGTTTCT TTCCCCAACA GCCTGTTGTT 501 GAGCCACCCC GTGCGCCGCG ACAATATTTT GGGCGACTAT GTCATCCATA 551 CGGTCGAAAT CCCCGTTCCC ATCCATTTGG ATTCGGATGA AGCCGTATGC 601 CGTCTGAAAG CCGTACTCGA GCCCTTGTGC GCGCCCTACA TCCCCGCCAT 651 CCAACGGsAT TTGGAAAACG TGCAGGCGGA AAAACTGTTT ATCACGCCCG 701 CCGCCAGACC GCGCGTTACC CGCGTGCCGT ACGATGACAA GGCATACCGC 751 ATCATCGTCC GCTTCGCTTC CCCCGTTTCA AAGCGGCTGG AAATCCAACA 801 GGCGGTTATG GACGAATTTT TGCGCGTACA ATACCGCCTG TTAAATCACC 851 CCGCCGGCTC CGAAACACTT TAA This corresponds to the amino acid sequence <SEQ ID 478; ORF 127>: ml27.pep 1 MEIWΝMLDTW LGAVPIRAEA VESVAAVAAL LLARALLLNI HFKRHPDFGI 51 ESKRRFLVAS RNITLLLVLF SLAFIWSAQI QTLALSMFAV AAAWVATKE 101 LIMCLSGSIL RSATQQYSVG DYIEINGLRG RWDINLLNT LMMQVGPNPL 151 VGQLAGTTVS FPNSLLLSHP VRRDNILGDY VIHTVEIPVP IHLDSDEAVC 201 RLKAVLEPLC APYIPAIQRX LENVQAEKLF ITPAARPRVT RVPYDDKAYR 251 IIVRFASPVS KRLEIQQAVM DEFLRVQYRL LNHPAGSETL * Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 127 shows 97.9% identity over a 290 aa overlap with a predicted ORF (ORF 127.ng) from N gonorrhoeae: ml27/gl27 10 20 30 40 50 60 ml27. pep MEIWΝMLDTWLGAVPIRAEAVESVAAVAALLLARALLLΝIHFKRHPDFGIESKRRFLVAS l l l l l l h l l M l l l l l h l l l l l l M I M I I I I M I I I h l l l l l l l l l l M I I I I I gl27 MEIWΝMLΝTWPDAVPIRAEAAESVAAVAALLLARALLLΝIHFRRHPDFGIESKRRFLVAS 10 20 30 40 50 60 70 80 90 100 110 120 ml27.pep RΝITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVWATKELIMCLSGSILRSATQQYSVG 11 M 111111111 M 11111 i 111111111 M E 1111111111111111 M M 11111 ] I gl27 RΝITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVWATKELIMCLSGSILRSATQQYSVG 70 80 90 100 110 120 130 140 150 160 170 180 ml27. pep DYIEINGLRGRWDINLLNTLMMQVGPNPLVGQLAGTTVSFPNSLLLSHPVRRDNILGDY gl27 DYIEINGLRGRWDINLLNTLMMQVGPNPLVGQLAGTTVSFPNSLLLSHPVRRDNILGDY 130 140 150 160 170 180 190 200 210 220 230 240 ml27 . pep VIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQRXLENVQAEKLFITPAARPRVT M M I M M M M M M M M M M M M M M M M M 1 1 1 1 I I I I I I 1 1 I I 1 1 1 M I gl27 VIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQRYLENVQAEKLFITPAARPRVT 190 200 210 220 230 240 250 260 270 280 290 ml27. pep RVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRVQYRLLNHPAGSETLX M M M I M M M M M M M M M M M M M M I M M M I I M M M I gl27 RVPYDDKAYRI IVRFASPVSKRLEIQQAVMDEFLRVQYRLLNHPAGSETLX 250 260 270 280 290 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 479>: al27. seq 1 ATGGAAATAT GGAATATGTT GGACACTTGG CTCGGTGCCG TCCCGATACG 51 TGCGGAGGCG GTCGAATCCG TGGCGGTGGT CGCGGCTTTG CTGCTGGCGC 101 GCGCCCTTCT GTTGAATATC CACTTCAAAC GGCATCCGGA TTTCGGCATC 151 GAAAGCAAGC GGCGGTTTTT GGTTGCCAGC CGCAATATAA CGCTGCTTTT 201 GGTGCTGTTT TCGCTGGCAT TTATCTGGTC GGCGCAAATC CAAACGCTGG 251 CTTTGTCGAT GTTTGCGGTG GCGGCGGCGG TCGTCGTGGC GACGAAGGAA 301 CTGATTATGT GTCTGTCGGG CAGCATTTTA AGGTCTGCCA CCCAGCAATA 351 CTCGGTCGGC GACTATATCG AAATCAACGG CCTGCGCGGG CGCGTGGTCG 401 ACATCAACCT GTTGAACACG CTGATGATGC AGGTCGGTCC GAACCCCTTG 451 GTCGGACAGC TTGCGGGAAC CACCGTTTCT TTCCCCAACA GCCTGTTGTT 501 GAGCCACCCC GTGCGCCGCG ACAATATTTT GGGCGACTAC GTCATCCATA 551 CGGTCGAAAT CCCGGTTCCC ATCCATTTGG ATTCGGATGA AGCCGTATGC 601 CGTCTGAAAG CCGTACTCGA GCCCTTGTGC GCGCCCTACA TCCCCGCCAT 651 CCAACGGCAT TTGGAAAACG TGCAGGCGGA AAAACTGTTT ATCACGCCCG 701 CCGCCAAACC GCGCGTTACC CGCGTGCCGT ACGATGACAA GGCATACCGC 751 ATCATCGTCC GCTTCGCCTC CCCCGTTTCA AAGCGGCTGG AAATCCAACA 801 GGCGGTTATG GACGAATTTT TGCGCGTACA ATACCGCCTG TTAAATTACC 851 CCGCCGGCTC CGAAACACTT TAA This corresponds to the amino acid sequence <SEQ ID 480; ORF 127.a>: al27.pep 1 MEIWΝMLDTW LGAVPIRAEA VESVAWAAL LLARALLLNI HFKRHPDFGI 51 ESKRRFLVAS RNITLLLVLF SLAFIWSAQI QTLALSMFAV AAAWVATKE 101 LIMCLSGSIL RSATQQYSVG DYIEINGLRG RWDINLLNT LMMQVGPNPL 151 VGQLAGTTVS FPNSLLLSHP VRRDNILGDY VIHTVEIPVP IHLDSDEAVC 201 RLKAVLEPLC APYIPAIQRH LENVQAEKLF ITPAARPRVT RVPYDDKAYR 251 IIVRFASPVS KRLEIQQAVM DEFLRVQYRL LNYPAGSETL * ml27/al27 98.6% identity in 290 aa overlap 10 20 30 40 50 60 ml27.pep MEIWNMLDTWLGAVPIRAEAVESVAAVAALLLARALLLNIHFKRHPDFGIESKRRFLVAS I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al27 MEIWNMLDTWLGAVPIRAEAVESVAWAALLLARALLLNIHFKRHPDFGIESKRRFLVAS 10 20 30 40 50 60 70 80 90 100 110 120 ml27.pep RNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVWATKELIMCLSGSILRSATQQYSVG I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I II I I I I I al27 RNITLLLVLFSLAFIWSA Q IQTLALSMFAVAAAVWATKELIMCLSGSILRSATQQYSVG 70 80 90 100 110 120 130 140 150 160 170 180 ml27. pep DYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVSFPNSLLLSHPVRRDNILGDY I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I al27 DYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVSFPNSLLLSHPVRRDNILGDY 130 140 150 160 170 180 190 200 210 220 230 240 ml27. pep VIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQRXLENVQAEKLFITPAARPRVT I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I al27 VIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQRHLENVQAEKLFITPAAKPRVT 190 200 210 220 230 240 250 260 270 280 290 ml27.pep RVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRVQYRLLNHPAGSETLX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I al27 RVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRVQYRLLNYPAGSETLX 250 260 270 280 290 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 481>: gl28.seq 1 atgattgaca acgCActgct ccacttgggc gaagaaccCC GTTTTaatca 51 aatccaaacc gaagACAtca AACCCGCCGT CCAAACCGCC ATCGCCGAAG 101 CGCGCGGACA AATCGCCGCC GTCAAAGCGC AAACGCACAC CGGCTGGGCG 151 AACACCGTCG AGCGTCTGAC CGGCATCACC GAACGCGTCG GCAGGATTTG 201 GGGCGTCGTG TCCCATCTCA ACTCCGTCGT CGACACGCCC GAACTGCGCG 251 CCGTCTATAA CGAACTGATG CCTGAAATCA CCGTCTTCTT CACCGAAATC 301 GGACAAGACA TCGAACTGTA CAACCGCTTC AAAACCATCA AAAATTCCCC 351 CGAATTTGCA ACGCTTTCCC CCGCACAAAA AACCAAGCTC GATCACGACC 401 TGCGCGATTT CGTATTGAGC GGCGCGGAAC TGCCGCCCGA ACGGCAGGCA 451 GAACTGGCAA AACTGCAAAC CGAAGGCGCG CAACTTTCCG CCAAATTCTC 501 CCAAAACGTC CTAGACGCGA CCGACGCGTT CGGCATTTAC TTTGACGATG 551 CCGCACCGCT TGCCGGCATT CCCGAAGACG CGCTCGCCAT GTTTGCCGCC 601 GCCGCGCAAA GCGAAGGCAA AACAGGTTAC AAAATCGGCT TGCAGATTCC 651 GCACTACCTT GCCGTTATCC AATACGCCGG CAACCGCGAA CTGCGCGAAC 701 AAATCTACCG CGCCTACGTT ACCCGTGCCA GCGAACTTTC AAACGACGGC 751 AAATTCGACA ACACCGCCAA CATCGACCGC ACGCTCGAAA ACGCATTGAA 801 AACCGccaaa cTGCTCGGCT TTAAAAATTA CGCCGAATTG TCGCTGGCAA 851 CCAAAATGGC GGACACGCCC GAACAGGTTT TAAACTTCCT GCACGACCTC 901 GCCCGCCGCG CCAAACCCTA CGCCGAAAAA GACCTCGCCG AAGTCAAAGC 951 CTTCGCCCGC GAACACCTCG GTCTCGCCGA CCCGCAGCCG TGGGACTTGA 1001 GCTACGCCGG CGAAAAACTG CGCGAAGCCA AATACGCATT CAGCGAAACC 1051 GAAGTCAAAA AATACTTCCC CGTCGGCAAA GTTCTGGCAG GCCTGTTCGC 1101 CCAAATCAAA AAACTCTACG GCATCGGATT CGCCGAAAAA ACCGTTCCCG 1151 TCTGGCACAA AGACGTGCGC TATTTTGAAT TGCAACAAAA CGGCAAAACC 1201 ATCGGCGGCG TTTATATGGA TTTGTACGCA CGCGAAGGCA AACGCGGCGG 1251 CGCGTGGATG AACGACtaca AAGGCCGCCG CCGCTTTGCC GACGgcacGC 1301 TGCAACTGCC CACCGCCTAC CTCGTCTGCA ACTTCGCCCC GCCCGTCGGC 1351 GGCAAAGAAG CGCGTTTAAG CCACGACGAA ATCCTCACCC TCTTCCACGA 1401 AacCGGCCAC GGACTGCACC ACCTGCTTAC CCAAGTGGAC GAACTGGGCG 1451 TGTCCGGCAT CAAcggcgtA GAATGGGACG CGGTCGAACT GCCCAGCCAG 1501 TTTATGGAAA ACTTCGTTTG GGAATACAAT GTATTGGCAC AAATGTCCGC 1551 CCACGAAGAA AccgGCGAGC CCCTGCCGAA AGAACTCTTC GACAAAATGC 1601 TcgcCGCCAA AAACTTCCAG CGCGGTATGT TCCTCGTCCG GCAAATGGAG 1651 TTCGCCCTCT TCGATATGAT GATTTACAGT GAAAGCGACG AATGCCGTCT 1701 GAAAAACTGG CAGCAGGTTT TAGACAGCGT GCGCAAAGAA GTcGCCGTCA 1751 TCCAACCGCC CGAATACAAC CGCTTCGCCA ACAGCTTCGG CCacatctTC 1801 GCcggcGGCT ATTCCGCAGG CTATTACAGC TACGCATGGG CCGAAGTCCt 1851 cAGCACCGAT GCCTACGCCG CCTTTGAAGA AAGcGACGac gtcGCCGCCA 1901 CAGGCAAACG CTTCTGGCAA GAAAtccttg ccgtcggcgg ctCCCGCAGC 1951 gcgGCGGAAT CCTTCAAAGC CTTCCGCGGA CGCGAACCGA GCATAGACGC 2001 ACTGCTGCGC CAaagcggtT TCGACAACGC gGCttgA This corresponds to the amino acid sequence <SEQ ID 482; ORF 128.ng>: gl28.pep 1 MIDNALLHLG EEPRFNQIQT EDIKPAVQTA IAEARGQIAA VKAQTHTGWA 51 NTVERLTGIT ERVGRIWGW SHLNSWDTP ELRAVYNELM PEITVFFTEI 101 GQDIELYNRF KTIKNSPEFA TLSPAQKTKL DHDLRDFVLS GAELPPERQA 151 ELAKLQTEGA QLSAKFSQNV LDATDAFGIY FDDAAPLAGI PEDALAMFAA 201 AAQSEGKTGY KIGLQIPHYL AVIQYAGNRE LREQIYRAYV TRASELSNDG 251 KFDNTANIDR TLENALKTAK LLGFKNYAEL SLATKMADTP EQVLNFLHDL 301 ARRAKPYAEK DLAEVKAFAR EHLGLADPQP WDLSYAGEKL REAKYAFSET 351 EVKKYFPVGK VLAGLFAQIK KLYGIGFAEK TVPVWHKDVR YFELQQNGKT 401 IGGVYMDLYA REGKRGGAWM NDYKGRRRFA DGTLQLPTAY LVCNFAPPVG 451 GKEARLSHDE ILTLFHETGH GLHHLLTQVD ELGVSGINGV EWDAVELPSQ 501 FMENFVWEYN VLAQMSAHEE TGEPLPKELF DKMLAAKNFQ RGMFLVRQME 551 FALFDMMIYS ESDECRLKNW QQVLDSVRKE VAVIQPPEYN RFANSFGHIF 601 AGGYSAGYYS YAWAEVLSTD AYAAFEESDD VAATGKRFWQ EILAVGGSRS 651 AAESFKAFRG REPSIDALLR QSGFDNAA* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 483>: ml28.seq (partial) 1 ATGACTGACA ACGCACTGCT CCATTTGGGC GAAGAACCCC GTTTTGATCA 51 AATCAAAACC GAAGACATCA AACCCGCCCT GCAAACCGCC ATCGCCGAAG 101 CGCGCGAACA AATCGCCGCC ATCAAAGCCC AAACGCACAC CGGCTGGGCA 151 AACACTGTCG AACCCCTGAC CGGCATCACC GAACGCGTCG GCAGGATTTG 201 GGGCGTGGTG TCGCACCTCA ACTGCGTCGC CGACACGCCC GAACTGCGCG 251 CCGTCTATAA CGAACTGATG CCCGAAATCA CCGTCTTCTT CACCGAAATC 301 GGACAAGACA TCGAGCTGTA CAACCGCTTC AAAACCATCA AAAATTCCCC 351 CGAATTCGAC ACCCTCTCCC CCGCACAAAA AACCAAACTC AACCAC 1 TACGCCAGCG AAAAACTGCG CGAAGCCAAA TACGCGTTCA GCGAAACCGA 51 wGTCAAAAAA TAyTTCCCyG TCGGCAAwGT ATTAAACGGA CTGTTCGCCC 101 AAmTCAAAAA ACTmTACGGC ATCGGATTTA CCGAAAAAAC yGTCCCCGTC 151 TGGCACAAAG ACGTGCGCTA TT TGAATTG CAACAAAACG GCGAAmCCAT 201 AGGCGGCGTT TATATGGATT TGTACGCACG CGAAGGCAAA CGCGGCGGCG 251 CGTGGATGAA CGACTACAAA GGCCGCCGCC GTTTTTCAGA CGGCACGCTG 301 CAAyTGCCCA CCGCCTACCT CGTCTGCAAC TTCGCCCCAC CCGTCGGCGG 351 CAGGGAAGCC CGCyTGAGCC ACGACGAAAT CCTCATCCTC TTCCACGAAA 401 CCGGACACGG GCTGCACCAC CTGCTTACCC AAGTGGACGA ACTGGGCGTA 451 TCCGGCATCA ACGGCGTAkA ATGGGACGCG GTCGAACTGC CCAGCCAGTT 501 TATGGAAAAT TTCGTTTGGG AATACAATGT CTTGGCACAA mTGTCAGCCC 551 ACGAAGAAAC CGGcgTTCCC yTGCCGAAAG AACTCTTsGA CAAA TGCTC 601 GCCGCCAAAA ACTTCCAAsG CGGCATGTTC yTsGTCCGGC AAwTGGAGTT 651 CGCCCTCTTT GATATGATGA TTTACAGCGA AGACGACGAA GGCCGTCTGA 701 AAAACTGGCA ACAGGTTTTA GACAGCGTGC GCAAAAAAGT CGCCGTCATC 751 CAGCCGCCCG AATACAACCG CTTCGCCTTG AGCTTCGGCC ACATCTTCGC 801 AGGCGGCTAT TCCGCAGCTn ATTACAGCTA CGCGTGGGCG GAAGTATTGA 851 GCGCGGACGC ATACGCCGCC TTTGAAGAAA GCGACGATGT CGCCGCCACA 901 GGCAAACGCT TTTGGCAGGA AATCCTCGCC GTCGGGGnAT CGCGCAGCGG 951 nGCAGAATCC TTCAAAGCCT TCCGCGGCCG CGAACCGAGC ATAGACGCAC 1001 TCTTGCGCCA CAGCGGTTTC GACAACGCGG TCTGA This corresponds to the amino acid sequence <SEQ ID 484; ORF 128>: ml28.pep (partial) 1 MTDΝALLHLG EEPRFDQIKT EDIKPALQTA IAEAREQIAA IKAQTHTGWA 51 ΝTVEPLTGIT ERVGRIWGW SHLΝCVADTP ELRAVYΝELM PEITVFFTEI 101 GQDIELYΝRF KTIKΝSPEFD TLSPAQKTKL ΝH // 1 YASEKLREAK YAFSETXVKK YFPVGXVLΝG LFAQXKKLYG IGFTEKTVPV 51 WHKDVRYXEL QQΝGEXIGGV YMDLYAREGK RGGAWMΝDYK GRRRFSDGTL 101 QLPTAYLVCΝ FAPPVGGREA RLSHDEILIL FHETGHGLHH LLTQVDELGV 151 SGIΝGVXWDA VELPSQFMEΝ FVWEYΝVLAQ XSAHEETGVP LPKELXDKXL 201 AAKΝFQXGMF XVRQXEFALF DMMIYSEDDE GRLKΝWQQVL DSVRKKVAVI 251 QPPEYΝRFAL SFGHIFAGGY SAAXYSYAWA EVLSADAYAA FEESDDVAAT 301 GKRFWQEILA VGXSRSGAES FKAFRGREPS IDALLRHSGF DNAV* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 128 shows 91.7% identity over a 475 aa overlap with a predicted ORF (ORF 128.ng) from N gonorrhoeae: ml28/gl28 10 20 30 40 50 60 gl28.pep MIDΝALLHLGEEPRFΝQIQTEDIKPAVQTAIAEARGQIAAVKAQTHTGWAΝTVERLTGIT ml28 MTDΝALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQTHTGWAΝTVEPLTGIT 10 20 30 40 50 60 70 80 90 100 110 120 gl28.pep ERVGRIWGWSHLΝSWDTPELRAVYΝELMPEITVFFTEIGQDIELYΝRFKTIKΝSPEFA ml28 ERVGRI GWSHLΝCVADTPELRAVYΝELMPEITVFFTEIGQDIELYΝRFKTIKΝSPEFD 70 80 90 100 110 120 130 140 150 160 170 180 gl28.pep TTLLSSPPAAQQKKTTKKLLDDHH1DLRDFVLSGAELPPERQAELAKLQTEGAQLSAKFSQΝVLDATDAFGIY 1 1111111111111111111 - • 11 ml28 TLSPAQKTKLΝH 130 II 340 350 360 gl28.pep YAGEKLREAKYAFSETEVKKYFPVGKVLAG ml28 YASEKLREAKYAFSETXVKKYFPVGXVLΝG 10 20 30 370 380 390 400 410 420 gl28.pep LFAQIKKLYGIGFAEKTVPVWHKDVRYFELQQΝGKTIGGVYMDLYAREGKRGGAWMΝDYK ml28 LFAQXKKLYGIGFTEKTVPVWHKDVRYXELQQΝGEXIGGVYMDLYAREGKRGGAWMΝDYK 40 50 60 70 80 90 430 440 450 460 470 480 gl28.pep GRRRFADGTLQLPTAYLVCΝFAPPVGGKEARLSHDEILTLFHETGHGLHHLLTQVDELGV ml28 GRRRFSDGTLQLPTAYLVCΝFAPPVGGREARLSHDEILILFHETGHGLHHLLTQVDELGV 100 110 120 130 140 150 490 500 510 520 530 540 gl28.pep SGIΝGVEWDAVELPSQFMEΝFVWEYΝVLAQMSAHEETGEPLPKELFDKMLAAKΝFQRGMF ml28 SGIΝGVXWDAVELPSQFMEΝFVWEYΝVLAQXSAHEETGVPLPKELXDKXLAAKΝFQXGMF 160 170 180 190 200 210 550 560 570 580 590 600 gl28.pep LVRQMEFALFDMMIYSESDECRLKΝWQQVLDSVRKEVAVIQPPEYΝRFAΝSFGHIFAGGY ml28 XVRQXEFALFDMMIYSEDDEGRLKΝWQQVLDSVRKKVAVIQPPEYΝRFALSFGHIFAGGY 220 230 240 250 260 270 610 620 630 640 650 660 gl28.pep SAGYYSYAWAEVLSTDAYAAFEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPS ml28 SAAXYSYAWAEVLSADAYAAFEESDDVAATGKRFWQEILAVGXSRSGAESFKAFRGREPS 280 290 300 310 320 330 670 679 gl28.pep IDALLRQSGFDNAAX M l l l h l l l l l h ml28 IDALLRHSGFDNAVX 340 The following partial DNA sequence was identified in N meningitidis <SEQ ID 485>: al28. seq 1 ATGACTGACA ACGCACTGCT CCATTTGGGC GAAGAACCCC GTTTTGATCA 51 AATCAAAACC GAAGACATCA AACCCGCCCT GCAAACCGCC ATTGCCGAAG 101 CGCGCGAACA AATCGCCGCC ATCAAAGCCC AAACGCACAC CGGCTGGGCA 151 AACACTGTCG AACCCCTGAC CGGCATCACC GAACGCGTCG GCAGGATTTG 201 GGGCGTGGTG TCGCACCTCA ACTCCGTCAC CGACACGCCC GAACTGCGCG 251 CCGCCTACAA TGAATTAATG CCCGAAATTA CCGTCTTCTT CACCGAAATC 301 GGACAAGACA TCGAGCTGTA CAACCGCTTC AAAACCATCA AAAACTCCCC 351 CGAGTTCGAC ACCCTCTCCC ACGCGCAAAA AACCAAACTC AACCACGATC 401 TGCGCGATTT CGTCCTCAGC GGCGCGGAAC TGCCGCCCGA ACAGCAGGCA 451 GAATTGGCAA AACTGCAAAC CGAAGGCGCG CAACTTTCCG CCAAATTCTC 501 CCAAAACGTC CTAGACGCGA CCGACGCGTT CGGCATTTAC TTTGACGATG 551 CCGCACCGCT TGCCGGCATT CCCGAAGACG CGCTCGCCAT GTTTGCCGCT 601 GCCGCGCAAA GCGAAGGCAA AACAGGCTAC AAAATCGGTT TGCAGATTCC 651 GCACTACCTC GCCGTCATCC AATACGCCGA CAACCGCAAA CTGCGCGAAC 701 AAATCTACCG CGCCTACGTT ACCCGCGCCA GCGAGCTTTC AGACGACGGC 751 AAATTCGACA ACACCGCCAA CATCGACCGC ACGCTCGAAA ACGCCCTGCA 801 AACCGCCAAA CTGCTCGGCT TCAAAAACTA CGCCGAATTG TCGCTGGCAA 851 CCAAAATGGC GGACACCCCC GAACAAGTTT TAAACTTCCT GCACGACCTC 901 GCCCGCCGCG CCAAACCCTA CGCCGAAAAA GACCTCGCCG AAGTCAAAGC 951 CTTCGCCCGC GAAAGCCTCG GCCTCGCCGA TTTGCAACCG TGGGACTTGG 1001 GCTACGCCGG CGAAAAACTG CGCGAAGCCA AATACGCATT CAGCGAAACC 1051 GAAGTCAAAA AATACTTCCC CGTCGGCAAA GTATTAAACG GACTGTTCGC 1101 CCAAATCAAA AAACTCTACG GCATCGGATT TACCGAAAAA ACCGTCCCCG 1151 TCTGGCACAA AGACGTGCGC TATTTTGAAT TGCAACAAAA CGGCGAAACC 1201 ATAGGCGGCG TTTATATGGA TTTGTACGCA CGCGAAGGCA AACGCGGCGG 1251 CGCGTGGATG AACGACTACA AAGGCCGCCG CCGTTTTTCA GACGGCACGC 1301 TGCAACTGCC CACCGCCTAC CTCGTCTGCA ACTTCACCCC GCCCGTCGGC 1351 GGCAAAGAAG CCCGCTTGAG CCATGACGAA ATCCTCACCC TCTTCCACGA 1401 AACCGGACAC GGCCTGCACC ACCTGCTTAC CCAAGTCGAC GAACTGGGCG 1451 TATCCGGCAT CAACGGCGTA GAATGGGACG CAGTCGAACT GCCCAGTCAG 1501 TTTATGGAAA ATTTCGTTTG GGAATACAAT GTCTTGGCGC AAATGTCCGC 1551 CCACGAAGAA ACCGGCGTTC CCCTGCCGAA AGAACTCTTC GACAAAATGC 1601 TCGCCGCCAA AAACTTCCAA CGCGGAATGT TCCTCGTCCG CCAAATGGAG 1651 TTCGCCCTCT TTGATATGAT GATTTACAGC GAAGACGACG AAGGCCGTCT 1701 GAAAAACTGG CAACAGGTTT TAGACAGCGT GCGCAAAGAA GTCGCCGTCG 1751 TCCGACCGCC CGAATACAAC CGCTTCGCCA ACAGCTTCGG CCACATCTTC 1801 GCAGGCGGCT ATTCCGCAGG CTATTACAGC TACGCGTGGG CGGAAGTATT 1851 GAGCGCGGAC GCATACGCCG CCTTTGAAGA AAGCGACGAT GTCGCCGCCA 1901 CAGGCAAACG CTTTTGGCAG GAAATCCTCG CCGTCGGCGG ATCGCGCAGC 1951 GCGGCAGAAT CCTTCAAAGC CTTCCGCGGA CGCGAACCGA GCATAGACGC 2001 ACTCTTGCGC CACAGCGGCT TCGACAACGC GGCTTGA This corresponds to the amino acid sequence <SEQ ID 486; ORF 128. a>: al28.pep 1 MTDΝALLHLG EEPRFDQIKT EDIKPALQTA IAEAREQIAA IKAQTHTGWA 51 ΝTVEPLTGIT ERVGRIWGW SHLΝSVTDTP ELRAAYΝELM PEITVFFTEI 101 GQDIELYΝRF KTIKΝSPEFD TLSHAQKTKL NHDLRDFVLS GAELPPEQQA 151 ELAKLQTEGA QLSAKFSQNV LDATDAFGIY FDDAAPLAGI PEDALAMFAA 201 AAQSEGKTGY KIGLQIPHYL AVIQYADNRK LREQIYRAYV TRASELSDDG 251 KFDNTANIDR TLENALQTAK LLGFKNYAEL SLATKMADTP EQVLNFLHDL 301 ARRAKPYAEK DLAEVKAFAR ESLGLADLQP WDLGYAGEKL REAKYAFSET 351 EVKKYFPVGK VLNGLFAQIK KLYGIGFTEK TVPVWHKDVR YFELQQNGET 401 IGGVYMDLYA REGKRGGAWM NDYKGRRRFS DGTLQLPTAY LVCNFTPPVG 451 GKEARLSHDE ILTLFHETGH GLHHLLTQVD ELGVSGINGV EWDAVELPSQ 501 FMENFVWEYN VLAQMSAHEE TGVPLPKELF DKMLAAKNFQ RGMFLVRQME 551 FALFDMMIYS EDDEGRLKNW QQVLDSVRKE VAWRPPEYN RFANSFGHIF 601 AGGYSAGYYS YAWAEVLSAD AYAAFEESDD VAATGKRFWQ EILAVGGSRS 651 AAESFKAFRG REPSIDALLR HSGFDNAA* /al28 66.0% identity in 677 aa overlap 10 20 30 40 50 60 ml28.pep MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQTHTGWANTVEPLTGIT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al28 MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQTHTGWANTVEPLTGIT 10 20 30 40 50 60 70 80 90 100 110 120 ml28.pep ERVGRIWGVVSHLNCVADTPELRAVYNELMPEITVFFTEIGQDIELYNRFKTIKNSPEFD I I I I I I I I I I I I I I I : I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al28 ERVGRIWGWSHLNSVTDTPELRAAYNELMPEITVFFTEIGQDIELYNRFKTIKNSPEFD 70 80 90 100 110 120 130 ml28.pep TLSPAQKTKLNH III llllllll al28 TLSHAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGAQLSAKFSQNVLDATDAFGIY 130 140 150 160 170 180 ml28.pep al28 FDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPHYLAVIQYADNRKLREQIYRAYV 190 200 210 220 230 240 ml28.pep al28 TRASELSDDGKFDNTANIDRTLENALQTAKLLGFKNYAELSLATKMADTPEQVLNFLHDL 250 260 270 280 290 300 140 150 ml28.pep YASEKLREAKYAFSETXVKKYFPVGX I I : I I I I I I I I I I I I I llllllll al28 ARRAKPYAEKDLAEVKAFARESLGLADLQPWDLGYAGEKLREAKYAFSETEVKKYFPVGK 310 320 330 340 350 360 160 170 180 190 200 210 ml28. pep VLNGLFAQXKKLYGIGFTEKTVPVWHKDVRYXELQQNGEXIGGVYMDLYAREGKRGGAWM llllllll I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I al28 VLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYFELQQNGETIGGVYMDLYAREGKRGGAWM 370 380 390 400 410 420 220 230 240 250 260 270 ml28. pep NDYKGRRRFSDGTLQLPTAYLVCNFAPPVGGREARLSHDEILILFHETGHGLHHLLTQVD I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I: I I I I I I I I I I I I I I I I I I I I I I I I I I I al28 NDYKGRRRFSDGTLQLPTAYLVCNFTPPVGGKEARLSHDEILTLFHETGHGLHHLLTQVD 430 440 450 460 470 480 280 290 300 310 320 330 ml28.pep ELGVSGINGVXWDAVELPSQFMENFVWEYNVLAQXSAHEETGVPLPKELXDKXLAAKNFQ I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I II lllllll al28 ELGVSGINGVEWDAVELPSQFMENFVWEYNVLAQMSAHEETGVPLPKELFDKMLAAKNFQ 490 500 510 520 530 540 340 350 360 370 380 390 ml28.pep XGMFXVRQXEFALFDMMIYSEDDEGRLKNWQQVLDSVRKKVAVIQPPEYNRFALSFGHIF III III I I I I I I I I I I I I I I I I I I I I I I I I I I I I II : I I I : : I I I I I I I I llllll al28 RGMFLVR Q MEFALFDMMIYSEDDEGRLKNWQQVLDSVRKEVAWRPPEYNRFANSFGHIF 550 560 570 580 590 600 400 410 420 430 440 450 ml28. pep AGGYSAAXYSYAWAEVLSADAYAAFEESDDVAATGKRFWQEILAVGXSRSGAESFKAFRG llllll: I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ll lllllllll al28 AGGYSAGYYSYAWAEVLSADAYAAFEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRG 610 620 630 640 650 660 460 470 ml28.pep REPSIDALLRHSGFDNAVX I I I I I I I I I I I I I I I I I : al28 REPSIDALLRHSGFDNAAX 670 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 487>: gl28-l.seq (partial) 1 ATGATTGACA ACGCACTGCT CCACTTGGGC GAAGAACCCC GTTTTAATCA 51 AATCAAAACC GAAGACATCA AACCCGCCGT CCAAACCGCC ATCGCCGAAG 101 CGCGCGGACA AATCGCCGCC GTCAAAGCGC AAACGCACAC CGGCTGGGCG 151 AACACCGTCG AGCGTCTGAC CGGCATCACC GAACGCGTCG GCAGGATTTG 201 GGGCGTCGTG TCCCATCTCA ACTCCGTCGT CGACACGCCC GAACTGCGCG 251 CCGTCTATAA CGAACTGATG CCTGAAATCA CCGTCTTCTT CACCGAAATC 301 GGACAAGACA TCGAACTGTA CAACCGCTTC AAAACCATCA AAAATTCCCC 351 CGAATTTGCA ACGCTTTCCC CCGCACAAAA AACCAAGCTC GATCACGACC 401 TGCGCGATTT CGTATTGAGC GGCGCGGAAC TGCCGCCCGA ACGGCAGGCA 451 GAACTGGCAA AACTGCAAAC CGAAGGCGCG CAACTTTCCG CCAAATTCTC 501 CCAAAACGTC CTAGACGCGA CCGACGCGTT CGGCATTTAC TTTGACGATG 551 CCGCACCGCT TGCCGGCATT CCCGAAGACG CGCTCGCCAT GTTTGCCGCC 601 GCCGCGCAAA GCGAAGGCAA AACAGGTTAC AAAATCGGCT TGCAGATTCC 651 GCACTACCTT GCCGTTATCC AATACGCCGG CAACCGCGAA CTGCGCGAAC 701 AAATCTACCG CGCCTACGTT ACCCGTGCCA GCGAACTTTC AAACGACGGC 751 AAATTCGACA ACACCGCCAA CATCGACCGC ACGCTCGAAA ACGCATTGAA 801 AACCGCCAAA CTGCTCGGCT TTAAAAATTA CGCCGAATTG TCGCTGGCAA 851 CCAAAATGGC GGACACGCCC GAACAGGTTT TAAACTTCCT GCACGACCTC 901 GCCCGCCGCG CCAAACCCTA CGCCGAAAAA GACCTCGCCG AAGTCAAAGC 951 CTTCGCCCGC GAACACCTCG GTCTCGCCGA CCCGCAGCCG TGGGACTTGA 1001 GCTACGCCGG CGAAAAACTG CGCGAAGCCA AATACGCATT CAGCGAAACC 1051 GAAGTCAAAA AATACTTCCC CGTCGGCAAA GTTCTGGCAG GCCTGTTCGC 1101 CCAAATCAAA AAACTCTACG GCATCGGATT CGCCGAAAAA ACCGTTCCCG 1151 TCTGGCACAA AGACGTGCGC TATTTTGAAT TGCAACAAAA CGGCAAAACC 1201 ATCGGCGGCG TTTATATGGA TTTGTACGCA CGCGAAGGCA AACGCGGCGG 1251 CGCGTGGATG AACGACTACA AAGGCCGCCG CCGCTTTGCC GACGGCACGC 1301 TGCAACTGCC CACCGCCTAC CTCGTCTGCA ACTTCGCCCC GCCCGTCGGC 1351 GGCAAAGAAG CGCGTTTAAG CCACGACGAA ATCCTCACCC TCTTCCACGA 1401 AACCGGCCAC GGACTGCACC ACCTGCTTAC CCAAGTGGAC GAACTGGGCG 1451 TGTCCGGCAT CAACGGCGTA AAA This corresponds to the amino acid sequence <SEQ ID 488; ORF 128-1. ng>: gl28-l.pep (partial) 1 MIDΝALLHLG EEPRFΝQIKT EDIKPAVQTA IAEARGQIAA VKAQTHTGWA 51 ΝTVERLTGIT ERVGRIWGW SHLΝSWDTP ELRAVYΝELM PEITVFFTEI 101 GQDIELYΝRF KTIKΝSPEFA TLSPAQKTKL DHDLRDFVLS GAELPPERQA 151 ELAKLQTEGA QLSAKFSQΝV LDATDAFGIY FDDAAPLAGI PEDALAMFAA 201 AAQSEGKTGY KIGLQIPHYL AVIQYAGΝRE LREQIYRAYV TRASELSΝDG 251 KFDΝTAΝIDR TLEΝALKTAK LLGFKΝYAEL SLATKMADTP EQVLΝFLHDL 301 ARRAKPYAEK DLAEVKAFAR EHLGLADPQP WDLSYAGEKL REAKYAFSET 351 EVKKYFPVGK VLAGLFAQIK KLYGIGFAEK TVPVWHKDVR YFELQQΝGKT 401 IGGVYMDLYA REGKRGGAWM ΝDYKGRRRFA DGTLQLPTAY LVCΝFAPPVG 451 GKEARLSHDE ILTLFHETGH GLHHLLTQVD ELGVSGIΝGV K The following partial DΝA sequence was identified in N meningitidis <SEQ ID 489>: ml28-l.seq 1 ATGACTGACA ACGCACTGCT CCATTTGGGC GAAGAACCCC GTTTTGATCA 51 AATCAAAACC GAAGACATCA AACCCGCCCT GCAAACCGCC ATCGCCGAAG 101 CGCGCGAACA AATCGCCGCC ATCAAAGCCC AAACGCACAC CGGCTGGGCA 151 AACACTGTCG AACCCCTGAC CGGCATCACC GAACGCGTCG GCAGGATTTG 201 GGGCGTGGTG TCGCACCTCA ACTCCGTCGC CGACACGCCC GAACTGCGCG 251 CCGTCTATAA CGAACTGATG CCCGAAATCA CCGTCTTCTT CACCGAAATC 301 GGACAAGACA TCGAGCTGTA CAACCGCTTC AAAACCATCA AAAATTCCCC 351 CGAATTCGAC ACCCTCTCCC CCGCACAAAA AACCAAACTC AACCACGATC 401 TGCGCGATTT CGTCCTCAGC GGCGCGGAAC TGCCGCCCGA ACAGCAGGCA 451 GAACTGGCAA AACTGCAAAC CGAAGGCGCG CAACTTTCCG CCAAATTCTC 501 CCAAAACGTC CTAGACGCGA CCGACGCGTT CGGCATTTAC TTTGACGATG 551 CCGCACCGCT TGCCGGCATT CCCGAAGACG CGCTCGCCAT GTTTGCCGCC 601 GCCGCGCAAA GCGAAAGCAA AACAGGCTAC AAAATCGGCT TGCAGATTCC 651 ACACTACCTC GCCGTCATCC AATACGCCGA CAACCGCGAA CTGCGCGAAC 701 AAATCTACCG CGCCTACGTT ACCCGCGCCA GCGAACTTTC AGACGACGGC 751 AAATTCGACA ACACCGCCAA CATCGACCGC ACGCTCGCAA ACGCCCTGCA 801 AACCGCCAAA CTGCTCGGCT TCAAAAACTA CGCCGAATTG TCGCTGGCAA 851 CCAAAATGGC GGACACGCCC GAACAAGTTT TAAACTTCCT GCACGACCTC 901 GCCCGCCGCG CCAAACCCTA CGCCGAAAAA GACCTCGCCG AAGTCAAAGC 951 CTTCGCCCGC GAAAGCCTGA ACCTCGCCGA TTTGCAACCG TGGGACTTGG 1001 GCTACGCCAG CGAAAAACTG CGCGAAGCCA AATACGCGTT CAGCGAAACC 1051 GAAGTCAAAA AATACTTCCC CGTCGGCAAA GTATTAAACG GACTGTTCGC 1101 CCAAATCAAA AAACTCTACG GCATCGGATT TACCGAAAAA ACCGTCCCCG 1151 TCTGGCACAA AGACGTGCGC TATTTTGAAT TGCAACAAAA CGGCGAAACC 1201 ATAGGCGGCG TTTATATGGA TTTGTACGCA CGCGAAGGCA AACGCGGCGG 1251 CGCGTGGATG AACGACTACA AAGGCCGCCG CCGTTTTTCA GACGGCACGC 1301 TGCAACTGCC CACCGCCTAC CTCGTCTGCA ACTTCGCCCC ACCCGTCGGC 1351 GGCAGGGAAG CCCGCCTGAG CCACGACGAA ATCCTCATCC TCTTCCACGA 1401 AACCGGACAC GGGCTGCACC ACCTGCTTAC CCAAGTGGAC GAACTGGGCG 1451 TATCCGGCAT CAACGGCGTA GAATGGGACG CGGTCGAACT GCCCAGCCAG 1501 TTTATGGAAA ATTTCGTTTG GGAATACAAT GTCTTGGCAC AAATGTCAGC 1551 CCACGAAGAA ACCGGCGTTC CCCTGCCGAA AGAACTCTTC GACAAAATGC 1601 TCGCCGCCAA AAACTTCCAA CGCGGCATGT TCCTCGTCCG GCAAATGGAG 1651 TTCGCCCTCT TTGATATGAT GATTTACAGC GAAGACGACG AAGGCCGTCT 1701 GAAAAACTGG CAACAGGTTT TAGACAGCGT GCGCAAAAAA GTCGCCGTCA 1751 TCCAGCCGCC CGAATACAAC CGCTTCGCCT TGAGCTTCGG CCACATCTTC 1801 GCAGGCGGCT ATTCCGCAGG CTATTACAGC TACGCGTGGG CGGAAGTATT 1851 GAGCGCGGAC GCATACGCCG CCTTTGAAGA AAGCGACGAT GTCGCCGCCA 1901 CAGGCAAACG CTTTTGGCAG GAAATCCTCG CCGTCGGCGG ATCGCGCAGC 1951 GCGGCAGAAT CCTTCAAAGC CTTCCGCGGC CGCGAACCGA GCATAGACGC 2001 ACTCTTGCGC CACAGCGGTT TCGACAACGC GGTCTGA This corresponds to the amino acid sequence <SEQ ID 490; ORF 128-1>: ml28-l.pep. 1 MTDNALLHLG EEPRFDQIKT EDIKPALQTA IAEAREQIAA IKAQTHTGWA 51 NTVEPLTGIT ERVGRIWGW SHLNSVADTP ELRAVYNELM PEITVFFTEI 101 GQDIELYNRF KTIKNSPEFD TLSPAQKTKL NHDLRDFVLS GAELPPEQQA 151 ELAKLQTEGA QLSAKFSQNV LDATDAFGIY FDDAAPLAGI PEDALAMFAA 201 AAQSESKTGY KIGLQIPHYL AVIQYADNRE LREQIYRAYV TRASELSDDG 251 KFDNTANIDR TLANALQTAK LLGFKNYAEL SLATKMADTP EQVLNFLHDL 301 ARRAKPYAEK DLAEVKAFAR ESLNLADLQP WDLGYAΞEKL REAKYAFSET 351 EVKKYFPVGK VLNGLFAQIK KLYGIGFTEK TVPVWHKDVR YFELQQNGET 401 IGGVYMDLYA REGKRGGAWM NDYKGRRRFS DGTLQLPTAY LVCNFAPPVG 451 GREARLSHDE ILILFHETGH GLHHLLTQVD ELGVSGINGV EWDAVELPSQ 501 FMENFVWEYN VLAQMSAHEE TGVPLPKELF DKMLAAKNFQ RGMFLVRQME 551 FALFDMMIYS EDDEGRLKNW QQVLDSVRKK VAVIQPPEYN RFALSFGHIF 601 AGGYSAGYYS YAWAEVLSAD AYAAFEESDD VAATGKRFWQ EILAVGGSRS 651 AAESFKAFRG REPSIDALLR HSGFDNAV* l28-l/gl28-l 94.5% identity in 491 aa overlap 10 20 30 40 50 60 gl28 - 1.pep MIDNALLHLGEEPRFNQIKTEDIKPAVQTAIAEARGQIAAVKAQTHTGWANTVERLTGIT I 1 1 1 h 1 1 1 1 1 1 1 1 1 1 1 1 1 M i l l ml28 - l MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQTHTGWANTVEPLTGIT 10 20 30 40 50 60 70 80 90 100 110 120 gl28 - l . pep ERVGRIWGWSHLNSWDTPELRAVYNELMPEITVFFTEIGQDIELYNRFKTIKNSPEFA ml28-l ERVGRIWGWSHLNSVADTPELRAVYNELMPEITVFFTEIGQDIELYNRFKTIKNSPEFD 70 80 90 100 110 120 130 140 150 160 170 180 gl28-1.pep TLSPAQKTKLDHDLRDFVLSGAELPPERQAELAKLQTEGAQLSAKFSQNVLDATDAFGIY ml28-1 TLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGAQLSAKFSQNVLDATDAFGIY 130 140 150 160 170 180 190 200 210 220 230 240 gl28-l .pep FDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPHYLAVIQYAGNRELREQIYRAYV ! I i 1 1 E 1 1 1 1 1 1 I ml28-l FDDAAPLAGIPEDALAMFAAAAQSESKTGYKIGLQIPHYLAVIQYADNRELREQIYRAYV 190 200 210 220 230 240 250 260 270 280 290 300 gl28-1.pep TRASELSNDGKFDNTANIDRTLENALKTAKLLGFKNYAELSLATKMADTPEQVLNFLHDL 11 h 11111111111111111111 II 111 II 111111 ml28 -1 TRASELSDDGKFDNTANIDRTLANALQTAKLLGFKNYAELSLATKMADTPEQVLNFLHDL 250 260 270 280 290 300 310 320 330 340 350 360 gl28-l.pep ARRAKPYAEKDLAEVKAFAREHLGLADPQPWDLSYAGEKLREAKYAFSETEVKKYFPVGK h i l l I M I h l h l l l l M I I I M I I M I I I M I I I ml28-l ARRAKPYAEKDLAEVKAFARESLNLADLQPWDLGYASEKLREAKYAFSETEVKKYFPVGK 310 320 330 340 350 360 370 380 390 400 410 420 gl28-1.pep VLAGLFAQIKKLYGIGFAEKTVPVWHKDVRYFELQQNGKTIGGVYMDLYAREGKRGGAWM II ml28-l VLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYFELQQNGETIGGVYMDLYAREGKRGGAWM 370 380 390 400 410 420 430 440 450 460 470 480 gl28-1.pep NDYKGRRRFADGTLQLPTAYLVCNFAPPVGGKEARLSHDEILTLFHETGHGLHHLLTQVD 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Itll 8-1 NDYKGRRRFSDGTLQLPTAYLVCNFAPPVGGREARLSHDEILILFHETGHGLHHLLTQVD 430 440 450 460 470 480 490 gl28-l.pep ELGVSGINGVK ml28-1 ELGVSGINGVEWDAVELPSQFMENFVWEYNVLAQMSAHEETGVPLPKELFDKMLAAKNFQ 490 500 510 520 530 540 The following partial DNA sequence was identified in N meningitidis <SEQ ID 491>: al28-l.seq 1 ATGACTGACA ACGCACTGCT CCATTTGGGC GAAGAACCCC GTTTTGATCA 51 AATCAAAACC GAAGACATCA AACCCGCCCT GCAAACCGCC ATTGCCGAAG 101 CGCGCGAACA AATCGCCGCC ATCAAAGCCC AAACGCACAC CGGCTGGGCA 151 AACACTGTCG AACCCCTGAC CGGCATCACC GAACGCGTCG GCAGGATTTG 201 GGGCGTGGTG TCGCACCTCA ACTCCGTCAC CGACACGCCC GAACTGCGCG 251 CCGCCTACAA TGAATTAATG CCCGAAATTA CCGTCTTCTT CACCGAAATC 301 GGACAAGACA TCGAGCTGTA CAACCGCTTC AAAACCATCA AAAACTCCCC 351 CGAGTTCGAC ACCCTCTCCC ACGCGCAAAA AACCAAACTC AACCACGATC 401 TGCGCGATTT CGTCCTCAGC GGCGCGGAAC TGCCGCCCGA ACAGCAGGCA 451 GAATTGGCAA AACTGCAAAC CGAAGGCGCG CAACTTTCCG CCAAATTCTC 501 CCAAAACGTC CTAGACGCGA CCGACGCGTT CGGCATTTAC TTTGACGATG 551 CCGCACCGCT TGCCGGCATT CCCGAAGACG CGCTCGCCAT GTTTGCCGCT 601 GCCGCGCAAA GCGAAGGCAA AACAGGCTAC AAAATCGGTT TGCAGATTCC 651 GCACTACCTC GCCGTCATCC AATACGCCGA CAACCGCAAA CTGCGCGAAC 01 AAATCTACCG CGCCTACGTT ACCCGCGCCA GCGAGCTTTC AGACGACGGC 751 AAATTCGACA ACACCGCCAA CATCGACCGC ACGCTCGAAA ACGCCCTGCA 801 AACCGCCAAA CTGCTCGGCT TCAAAAACTA CGCCGAATTG TCGCTGGCAA 851 CCAAAATGGC GGACACCCCC GAACAAGTTT TAAACTTCCT GCACGACCTC 901 GCCCGCCGCG CCAAACCCTA CGCCGAAAAA GACCTCGCCG AAGTCAAAGC 951 CTTCGCCCGC GAAAGCCTCG GCCTCGCCGA TTTGCAACCG TGGGACTTGG 1001 GCTACGCCGG CGAAAAACTG CGCGAAGCCA AATACGCATT CAGCGAAACC 1051 GAAGTCAAAA AATACTTCCC CGTCGGCAAA GTATTAAACG GACTGTTCGC 1101 CCAAATCAAA AAACTCTACG GCATCGGATT TACCGAAAAA ACCGTCCCCG 1151 TCTGGCACAA AGACGTGCGC TATTTTGAAT TGCAACAAAA CGGCGAAACC 1201 ATAGGCGGCG TTTATATGGA TTTGTACGCA CGCGAAGGCA AACGCGGCGG 1251 CGCGTGGATG AACGACTACA AAGGCCGCCG CCGTTTTTCA GACGGCACGC 1301 TGCAACTGCC CACCGCCTAC CTCGTCTGCA ACTTCACCCC GCCCGTCGGC 1351 GGCAAAGAAG CCCGCTTGAG CCATGACGAA ATCCTCACCC TCTTCCACGA 1401 AACCGGACAC GGCCTGCACC ACCTGCTTAC CCAAGTCGAC GAACTGGGCG 1451 TATCCGGCAT CAACGGCGTA GAATGGGACG CAGTCGAACT GCCCAGTCAG 1501 TTTATGGAAA ATTTCGTTTG GGAATACAAT GTCTTGGCGC AAATGTCCGC 1551 CCACGAAGAA ACCGGCGTTC CCCTGCCGAA AGAACTCTTC GACAAAATGC 1601 TCGCCGCCAA AAACTTCCAA CGCGGAATGT TCCTCGTCCG CCAAATGGAG 1651 TTCGCCCTCT TTGATATGAT GATTTACAGC GAAGACGACG AAGGCCGTCT 1701 GAAAAACTGG CAACAGGTTT TAGACAGCGT GCGCAAAGAA GTCGCCGTCG 1751 TCCGACCGCC CGAATACAAC CGCTTCGCCA ACAGCTTCGG CCACATCTTC 1801 GCAGGCGGCT ATTCCGCAGG CTATTACAGC TACGCGTGGG CGGAAGTATT 1851 GAGCGCGGAC GCATACGCCG CCTTTGAAGA AAGCGACGAT GTCGCCGCCA 1901 CAGGCAAACG CTTTTGGCAG GAAATCCTCG CCGTCGGCGG ATCGCGCAGC 1951 GCGGCAGAAT CCTTCAAAGC CTTCCGCGGA CGCGAACCGA GCATAGACGC 2001 ACTCTTGCGC CACAGCGGCT TCGACAACGC GGCTTGA This corresponds to the amino acid sequence <SEQ ID 492; ORF 128-1. a>: al28-l.pep 1 MTDNALLHLG EEPRFDQIKT EDIKPALQTA IAEAREQIAA IKAQTHTGWA 51 NTVEPLTGIT ERVGRIWGW SHLNSVTDTP ELRAAYNELM PEITVFFTEI 101 GQDIELYNRF KTIKNSPEFD TLSHAQKTKL NHDLRDFVLS GAELPPEQQA 151 ELAKLQTEGA QLSAKFSQNV LDATDAFGIY FDDAAPLAGI PEDALAMFAA 201 AAQSEGKTGY KIGLQIPHYL AVIQYADNRK LREQIYRAYV TRASELSDDG 251 KFDNTANIDR TLENALQTAK LLGFKNYAEL SLATKMADTP EQVLNFLHDL 301 ARRAKPYAEK DLAEVKAFAR ESLGLADLQP WDLGYAGEKL REAKYAFSET 351 EVKKYFPVGK VLNGLFAQIK KLYGIGFTEK TVPVWHKDVR YFELQQNGET 401 IGGVYMDLYA REGKRGGAWM NDYKGRRRFS DGTLQLPTAY LVCNFTPPVG 451 GKEARLSHDE ILTLFHETGH GLHHLLTQVD ELGVSGINGV EWDAVELPSQ 501 FMENFVWEYN VLAQMSAHEE TGVPLPKELF DKMLAAKNFQ RGMFLVRQME 551 FALFDMMIYS EDDEGRLKNW QQVLDSVRKE VAWRPPEYN RFANSFGHIF 601 AGGYSAGYYS YAWAEVLSAD AYAAFEESDD VAATGKRFWQ EILAVGGSRS 651 AAESFKAFRG REPSIDALLR HSGFDNAA* ml28-l/al28-l 97.8% identity in 677 aa overlap 10 20 30 40 50 60 al28-1.pep MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQTHTGWANTVEPLTGIT ml28 - 1 MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQTHTGWANTVEPLTGIT 10 20 30 40 50 60 70 80 90 100 110 120 al28 -l . pep ERVGRIWGWSHLNSVTDTPELRAAYNELMPEITVFFTEIGQDIELYNRFKTIKNSPEFD ml28 -1 ERVGRIWGWΞHLNSVADTPELRAVYNELMPEITVFFTEIGQDIELYNRFKTIKNSPEFD 70 80 90 100 110 120 130 140 150 160 170 180 al28 - 1 . pep TLSHAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGAQLSAKFSQNVLDATDAFGI Y I I I ml28-1 TLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGAQLSAKFSQNVLDATDAFGIY 130 140 150 160 170 180 190 200 210 220 230 240 al28-l .pep FDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPHYLAVIQYADNRKLREQIYRAYV ml28-l FDDAAPLAGIPEDALAMFAAAAQSESKTGYKIGLQIPHYLAVIQYADNRELREQIYRAYV 190 200 210 220 230 240 250 260 270 280 290 300 al28 -1.pep TRASELSDDGKFDNTANIDRTLENALQTAKLLGFKNYAELSLATKMADTPEQVLNFLHDL 111 r 11 E a 11111111 i 11111 L i 11 j 111111 E 11 i ml28 -1 TRASELSDDGKFDNTANIDRTLANALQTAKLLGFKNYAELSLATKMADTPEQVLNFLHDL 250 260 270 280 290 300 310 320 330 340 350 360 al28 -1. pep ARRAKPYAEKDLAEVKAFARESLGLADLQPWDLGYAGEKLREAKYAFΞETEVKKYFPVGK ml28 -1 ARRAKPYAEKDLAEVKAFAREΞLNLADLQPWDLGYASEKLREAKYAFSETEVKKYFPVGK 310 320 330 340 350 360 370 380 390 400 410 420 al28-l.pep VLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYFELQQNGETIGGVYMDLYAREGKRGGAWM ml28 -1 VLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYFELQQNGETIGGVYMDLYAREGKRGGAWM 370 380 390 400 410 420 430 440 450 460 470 480 al28-l.pep NDYKGRRRFSDGTLQLPTAYLVCNFTPPVGGKEARLSHDEILTLFHETGHGLHHLLTQVD ml28 -1 NDYKGRRRFSDGTLQLPTAYLVCNFAPPVGGREARLSHDEILILFHETGHGLHHLLTQVD 430 440 450 460 470 480 490 500 510 520 530 540 al28 -1.pep ELGVSGINGVEWDAVELPSQFMENFVWEYNVLAQMSAHEETGVPLPKELFDKMLAAKNFQ ml28-l ELGVSGINGVEWDAVELPSQFMENFVWEYNVLAQMSAHEETGVPLPKELFDKMLAAKNFQ 490 500 510 520 530 540 550 560 570 580 590 600 al28-l.pep RGMFLVRQMEFALFDMMIYΞEDDEGRLKNWQQVLDSVRKEVAWRPPEYNRFANΞFGHIF ml28-l RGMFLVRQMEFALFDMMIYSEDDEGRLKNWQQVLDSVRKKVAVIQPPEYNRFALSFGHIF 550 560 570 580 590 600 610 620 630 640 650 660 al28-l.pep AGGYSAGYYSYAWAEVLSADAYAAFEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRG ml28 -1 AGGYSAGYYSYAWAEVLSADAYAAFEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRG 610 620 630 640 650 660 670 679 al28-l.pep REPSIDALLRHSGFDNAAX Ill M ml28-l REPSIDALLRHSGFDNAVX 670 al28-l/ P44573 sp|P44573|OPDA_HAEIN OLIGOPEPTIDASE A >gi | 1075082 | pir | | C64055 oligopeptidase A (prlC) homolog - Haemophilus influenzae (strain Rd KW20) >gi 11573174 (U32706) oligopeptidase A (prlC) [Haemophilus influenzae Rd] Length = 681 Score = 591 bits (1507) , Expect = e-168 Identities = 309/677 (45%), Positives = 415/677 (60%), Gaps = 4/677 (0%) Query: 4 NALLHLGEEPRFDQIKTEDIKPALQTXXXXXXXXXXXXXXXTHTGWANTVEPLTGITERV 63 N LL++ P F QIK E I+PA++ H W N + PLT +R+ Sbjct: 5 NPLLNIQGLPPFSQIKPEHIRPAVEKLIQDCRNTIEQVLKQPHFTWENFILPLTETNDRL 64 Query: 64 GRIWGWSHLNSVTDTPELRAAYNELMPEITVFFTEIGQDIELYNRFKTIKNSPEFDTLS 123 R W VSHLNSV ++ ELR AY +P ++ + T +GQ LYN + +KNS EF S Sbjct: 65 NRAWΞPVSHLNSVKNSTELREAYQTCLPLLSEYSTWVGQHKGLYNAYLALKNSAEFADYS 124 Query: 124 HAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGAQLSAKFSQNVLDATDAFGIYFDD 183 AQK + + LRDF LSG L E+Q ++ ++L+++FS NVLDAT + ++ Sbjct: 125 IAQKKAIENΞLRDFELSGIGLSEEKQQRYGEIVARLSELNSQFSNNVLDATMGWEKLIEN 184 Query: 184 AAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPHYLAVIQYADNRKLREQIYRAYVTRA 243 A LAG+PE AL +A+S+G GY+ L+IP YL V+ Y +NR LRE++YRAY TRA Sbjct: 185 EAELAGLPESALQAAQQSAESKGLKGYRFTLEIPΞYLPVMTYCENRALREEMYRAYATRA 244 Query: 244 SELSDD-GKFDNTANIDRTLENALQTAKLLGFKNYAELSLATKMADTPEQVLNFLHDLAR 302 SE + GK+DN+ ++ L ++ AKLLGF Y ELSLATKMA+ P+QVL+FL LA Sbjct: 245 SEQGPNAGKWDNSKVMEEILTLRVELAKLLGFNTYTELSLATKMAENPQQVLDFLDHLAE 304 Query: 303 RAKPYAEKDLAEVKAFARESLGLADLQPWDLGYAGEKLREAKYAFSETEVKKYFPVGKVL 362 RAKP EK+L E+K + + G+ +L PWD+G+ EK ++ YA ++ E++ YFP +V+ Sbjct: 305 RAKPQGEKELQELKGYCEKEFGVTELAPWDIGFYSEKQKQHLYAINDEELRPYFPENRVI 364 Query: 363 NGLFAQIKKLYGIGFTE-KTVPVWHKDVRYFEL-QQNGETIGGVYMDLYAREGKRGGAWM 420 +GLF IK+++ I E K V WHKDVR+F+L +N + G Y+DLYARE KRGGAWM Sbjct: 365 SGLFELIKRIFNIRAVERKGVDTWHKDVRFFDLIDENDQLRGSFYLDLYAREHKRGGAWM 424 Query: 421 NDYKGRRRFSDGTLQLPTAYLVCNFTPPVGGKEARLSHDEIXXXXXXXXXXXXXXXXQVD 480 +D GR+R DG+++ P AYL CNF P+G K A +H+E+ Q+D Sbjct: 425 DDCIGRKRKLDGSIETPVAYLTCNFNAPIGNKPALFTHNEVTTLFHEFGHGIHHMLTQID 484 Query: 481 ELGVSGINGVEWDAVELPΞQFMENFVWEYNVLAQMSAHEETGVPLPKELFDKMLAAKNFQ 540 V+GINGV WDAVELPSQFMEN+ WE LA +Ξ H ETG PLPKE ++L AKNFQ Sbjct: 485 VSDVAGINGVPWDAVELPSQFMEN CWEEEALAFISGHYETGEPLPKEKLTQLLKAKNFQ 544 Query: 541 RGMFLVRQMEFALFDMMIYΞEDDEGRLKNWQQVLDSVRKEVAWRPPEYNRFANSFGHIF 600 MF++RQ+EF +FD ++ D + L SV+ +VAV++ ++ R +SF HIF Sbjct: 545 AAMFILRQLEFGIFDFRLHHTFDAEKTNQILDTLKSVKSQVAVIKGVDWARAPHSFSHIF 604 Query: 601 XXXXXXXXXXXXWAEVLSADAYAAFEEΞDDV-AATGKRFWQEILAVGGSRSAAESFKAFR 659 WAEVLSADAY+ FEE TGK F EIL GGΞ E FK FR Sbjct: 605 AGGYAAGYYSYLWAEVLSADAYSRFEEEGIFNPITGKSFLDEILTRGGSEEPMELFKRFR 664 Query: 660 GREPΞIDALLRHSGFDN 676 GREP +DALLRH G N Sbjct: 665 GREPQLDALLRHKGIMN 681 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 493>: gl29.seq 1 ATGCTTTCAC CTCCTCGGCG TAAAACGGCG GCACATCAAT CAAGCCGTCT 51 TTCATTTGCG TGCGGAAAAA ATGCGGCGTG TTGCCGTGAT CAAAATCAAT 101 ATCGTGCAGC ATCCAGCCCA AATCGCGGTT TGCCTCGCTT TCCGATAACG 151 CCGACGGCGG CAGCGGTTCA CCCTTATCCG CGCTTTCGCC ATTTGCCCTT 201 TCAGGCTGCG GGCATAGGGG CGGAACAGGC GGCGGTCGAA TCCTGTTTCA 251 TCCGGACAAA CGCGTTGGCA GTCGGAAAAT CCGGCCGGCC GTGTCAAATA 301 ATGCGTTACT TTGGCCGGGT CTTGTCCTTT GTAAGCGGCG GTCTTTTTTT 351 GCGCGCCATC CGCATCTGTT TGGGCGCATG GCAAACGGCG GCTGCCGTAC 401 AATCAAAATG TTTGGCGATT TCATGCAGAC AGGCATCCGG ATGCCGCCCG 451 ACATATCGAG CCGGTTTTTG CCTATCCGAT TTGGCGGCAT TTAGGCCGGT 501 AACTTGA This corresponds to the amino acid sequence <SEQ ID 494; ORF 129.ng>: gl29.pep 1 MLSPPRRKTA AHQSSRLSFA CGKΝAACCRD QΝQYRAASSP ΝRGLPRFPIT 51 PTAAAVHPYP RFRHLPFQAA GIGAEQAAVE SCFIRTΝALA VGKSGRPCQI 101 MRYFGRVLSF VSGGLFLRAI RICLGAWQTA AAVQSKCLAI SCRQASGCRP 151 TYRAGFCLSD LAAFRPVT* The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 495>: ml29.seq (partial) 1 .. TATCTGCGCT TTCACTATTT GCCCTTTCAG GCTGCGGGCA TAGGGACGGA 51 ACAGGTAGCG GTCAAATCCT GTTTCATCCA AATAAACACG TTGGTAGTCG 101 GAAAATTCGG CCGGCTGTGT CAAATAATGC GTTACTTTGG CCGGGTCTTG 151 TTCTTTGTAA GTGGTGGTCT TTTTTTGCGC GTTATCCCCA TCTGTTTGAG 201 TGCATAGCAA ATGGTGGCTG CCGTACAATC AAAATGTTTG GCGATTTCAT 251 GCAGATAGGC ATCCGGGTGT TGCCCAACAT ATTGAGCCGG TTTTTGCCTA 301 TCCGATTTGA CGGCATTTAG ACCGGTAACT TGA This corresponds to the amino acid sequence <SEQ ID 496; ORF 129>: ml29.pep (partial) 1 ..YLRFHYLPFQ AAGIGTEQVA VKSCFIQIΝT LWGKFGRLC QIMRYFGRVL 51 FFVSGGLFLR VIPICLSAXQ MVAAVQSKCL AISCRXASGC CPTYXAGFCL 101 SDLTAFRPVT * Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 129 shows 79.1% identity over a 110 aa overlap with a predicted ORF (ORF 129.ng) from N gonorrhoeae: ml29/gl29 10 20 30 ml29 -pep YLRFHYLPFQAAGIGTEQVAVKSCFIQIΝT I I h M M M M I h l h l h l l l h h gl29 RDQΝQYRAASSPΝRGLPRFPITPTAAAVHPYPRFRHLPFQAAGIGAEQAAVESCFIRTΝA 30 40 50 60 70 80 40 50 60 70 80 90 ml29.pep LWGKFGRLCQIMRYFGRVLFFVSGGLFLRVIPICLSAXQMVAAVQSKCLAISCRXASGC hill II llhl I MM gl29 LAVGKSGRPCQIMRYFGRVLSFVSGGLFLRAIRICLGAWQTAAAVQSKCLAISCRQASGC 90 100 110 120 130 140 100 110 ml29. pep CPTYXAGFCLSDLTAFRPVTX III gl29 RPTYRAGFCLSDLAAFRPVTX 150 160 The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 497>: al29.seq (partial) 1 TATCTGCGCT TTCACTATTT GCCCTTTCAG GCTGCGGGCA TAGGGACGGA 51 ACAGGTAGCG GTCAAATCCT GTTTCATCCA AATAAACACG TTGGTAGTCG 101 GAAAATTCGG CCAGCTGTGT CAAATAATGC GTTACTTTGG CCGGGTCTTG 151 TTCTTTGTAA GTGGTGGTCT TTTTTTGCGC GTTATCCCCA TCTGTTTGAG 201 TGCATAGCAA ATGGTGGCTG CCGTACAATC AAAATGTTTG GCGATTTCAT 251 GCAGATAGGC ATCCTGGTGT TGCCCAACAT ATTGAGCCGG TTTTTGCCTA 301 TCCGATTTGA CGGCATTTAG ACCGGTAACT TGA This corresponds to the amino acid sequence <SEQ ID 498; ORF 129. a>: al29.pep (partial) 1 YLRFHYLPFQ AAGIGTEQVA VKSCFIQIΝT LWGKFGQLC QIMRYFGRVL 51 FFVSGGLFLR VIPICLSA*Q MVAAVQSKCL AISCR*ASWC CPTY*AGFCL 101 SDLTAFRPVT * ml29/al29 98.2% identity in 110 aa overlap 10 20 30 40 50 60 ml29.pep YLRFHYLPFQAAGIGTEQVAVKSCFIQIΝTLWGKFGRLCQIMRYFGRVLFFVSGGLFLR I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I al29 YLRFHYLPFQAAGIGTEQVAVKSCFIQIΝTLVVGKFGQLCQIMRYFGRVLFFVSGGLFLR 10 20 30 40 50 60 70 80 90 100 110 ml29.pep VIPICLSAXQMVAAVQSKCLAISCRXASGCCPTYXAGFCLSDLTAFRPVTX I I I II I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I al29 VIPICLSAXQMVAAVQSKCLAISCRXASWCCPTYXAGFCLSDLTAFRPVTX 70 80 90 100 110 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 499>: gl30.seq 1 ATGAAACAAC TCCGCGACAA CAAAGCCCAA GGCTCTGCAC TGTTTACCCT 51 TGTGAGCGGT ATCGTTATTG TTATTGCAGT CCTTTATTTC CTGATTAAGC 101 TGGCGGGCAG TGGATCGTTC GGCGATGTCG ATGCCACTAC GGAAGCGGCA 151 ACGCAGACCC GCATCCAGCC TGTCGGACAA TTGACGATGG GTGACGGCAT 201 CCCCGTCGGC GAACGCCAAG GCGAACAGAT TTTCGGCAAA ATCTGTATCC 251 AATGCCACGC GGCGGACAGC AATGTGCCGA ACGCTCCGAA ACTGGAACAC 301 AACGGCGACT GGGCGCCGCG TATCGCGCAA GGCTTCGATA CCTTGTTCCA 351 ACACGCGCTG AACGGCTTTA ACGCCATGCC TGCCAAAGGC GGTGCGGCAG 401 ACCTGACCGA TCAGGAACTC AAACGGGCGA TTACCTACAT GGCGAATAAA 451 AGCGGCGGTT CTTTCCCGAA TCCTGATGAG GCTGCGCCTG CCGACAATGC 501 CGCTTCAGGA ACAGCTTCTG CTCCTGCCGA TAGTGCAGCT CCGGCAGAAG 551 CGAAGGCAGA AGACAAGGGT GCGGCAGCCC CTGCGGTCGG CGTTGACGGT 601 AAAAAAGTCT TCGAAGCAAC CTGTCAGGTG TGCCACGGCG GTTCGATTCC 651 CGGTATTCCC GGCATAGGCA AAAAAGACGA TTGGGCACCG CGTATCAAAA 701 AAGGCAAAGA AACCTTGCAC AAACATGCCC TTGAAGGCTT TAACGCGATG 751 CCGGCCAAAG GCGGCAATGC AGGTTTGAGC GATGACGAAG TCAAAGCGGC 801 TGTTGACTAT ATGGCAAACC AATCCGGTGC AAAATTCTAA This corresponds to the amino acid sequence <SEQ ID 500; ORF 130.ng>: gl30.pep 1 MKQLRDNKAQ GSALFTLVSG IVIVIAVLYF LIKLAGSGSF GDVDATTEAA 51 TQTRIQPVGQ LTMGDGIPVG ERQGEQIFGK ICIQCHAADS NVPNAPKLEH 101 NGDWAPRIAQ GFDTLFQHAL NGFNAMPAKG GAADLTDQEL KRAITYMANK 151 SGGSFPNPDE AAPADNAASG TASAPADSAA PAEAKAEDKG AAAPAVGVDG 201 KKVFEATCQV CHGGSIPGIP GIGKKDDWAP RIKKGKETLH KHALEGFNAM 251 PAKGGNAGLS DDEVKAAVDY MANQSGAKF* The following partial DNA sequence was identified in N meningitidis <SEQ ID 501>: ml30.seq (partial) 1 ..GGCGAACAGA TTTTCGGCAA AATCTGTATC CAATGCCACG CGGCGGACAG 51 CAATGTGCCG AACGCTCCGA AACTGGAACA CAACGGCGAT TrGGCACCGC 101 GTATCGgCAA GGCTTCGATA CCTTGTTCCA ACACGCGCTG AACGGCTTTA 151 ACGCCATGCC TGCAAAAGGC GGTGCGGCAG ACCTGACCGA TCAGGAACTT 201 AAACGGGCGA TTACTTACAT GGCGAACAAA AGCGGCGGTT CTTTCCCGAA 251 TCCTGATGAG GCTGCGCCTG CCGACAATGC CGCTTCAGGA ACAGCTTCTG 301 CTCCTGCCGA TAGTGCAGCT CCGGCAGAAG CGAAGGCAGA AGACAAGGGT 351 GCGGCAcCCC TGCGGTCGGC GTTGACGGTA AAAAAGTCTT CGAAGCAACC 401 TGTCAGGTGT GCCACGGCGG TTCGATTCCC GGTATTCCCG GCATAGGCAA 451 AAAAGACGAT TGGGCACCGC GTATCAAAAA AGGCAAAGAA ACCTTGCACA 501 AACACGCCCT TGAAGGCTTT AACGCGATGC CTGCCAAArG CGgCAATGCA 551 GGTTTGAGCG ATGACGAAgT CAAAGCGGCT GTTGACTATA TGGCAAACCA 601 ATCCGGTGCA AAATTCTAA This corresponds to the amino acid sequence <SEQ LD 502; ORF 130>: ml30.pep (partial) 1 ..GEQIFGKICI QCHAADSΝVP ΝAPKLEHΝGD XAPRIQGFDT LFQHALΝGFΝ 51 AMPAKGGAAD LTDQELKRAI TYMAΝKSGGS FPΝPDEAAPA DΝAASGTASA 101 PADSAAPAEA KAEDKGAAPA VGVDGKKVFE ATCQVCHGGS IPGIPGIGKK 151 DDWAPRIKKG KETLHKHALE GFΝAMPAKXG ΝAGLSDDEVK AAVDYMAΝQS 201 GAKF* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 130 shows 98.1% identity over a 206 aa overlap with a predicted ORF (ORF 130.ng) from N gonorrhoeae: ml30/gl30 10 20 30 ml30.pep GEQIFGKICIQCHAADSΝVPΝAPKLEHΝGD 111 i 1111 II 11 II 11111111 M I M M 1 gl30 DATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSΝVPΝAPKLEHΝGD 50 60 70 80 90 100 40 50 60 70 80 89 ml30. pep XAPRI -QGFDTLFQHALΝGFΝAMPAKGGAADLTDQELKRAITYMAΝKSGGSFPΝPDEAAP MM MMMMMMMMMMMMMMMMMMMMIMMMMMMI gl30 WAPRIAQGFDTLFQHALΝGFΝAMPAKGGAADLTDQELKRAITYMAΝKSGGSFPΝPDEAAP 110 120 130 140 150 160 90 100 110 120 130 140 ml30.pep ADNAASGTASAPADSAAPAEAKAEDKGAA-PAVGVDGKKVFEATCQVCHGGSIPGIPGIG M M M M M M M M I M M M M M M I I I M I I 1 1 1 I I I I I M I I I I I I M 1 1 1 1 1 gl30 ADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPGIPGIG 170 180 190 200 210 220 150 160 170 180 190 200 ml30. pep KKDDWAPRIKKGKETLHKHALEGFNAMPAKXGNAGLSDDEVKAAVDYMANQSGAKFX M M I M M M M M M M I M M M M M I I I I I I I I I I I M 1 1 1 I I 1 1 M 1 1 I I gl30 KKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSGAKFX 230 240 250 260 270 280 The following partial DNA sequence was identified in N meningitidis <SEQ ID 503>: al30. seq 1 ATGAAACAAC TCCGCGACAA CAAAGCCCAA GGCTCTGCAC TGTTTACCCT 51 TGTGAGCGGT ATCGTTATTG TTATTGCAGT CCTTTATTTC CTGATTAAGC 101 TGGCGGGCAG CGGCTCGTTC GGCGATGTCG ATGCCACTAC GGAAGCAGCA 151 ACGCAGACCC GTATCCAGCC TGTCGGACAA TTGACGATGG GCGACGGCAT 201 CCCCGTCGGC GAACGCCAAG GCGAACAGAT TTTCGGCAAA ATCTGTATCC 251 AATGCCACGC GGCGGACAGC AATGTGCCGA ACGCTCCGAA ACTGGAACAC 301 AACGGCGATT GGGCGCCGCG TATCGCGCAA GGCTTCGATA CCTTGTTCCA 351 ACACGCGCTG AACGGCTTTA ACGCCATGCC TGCCAAAGGC GGTGCGGTAG 401 ACCTGACCGA TCAGGAACTC AAACGGGCGA TTACTTACAT GGCGAACAAA 451 AGCGGCGGTT CTTTCCCGAA TCCTGATGAG GCTGCGCCTG CCGACAATGC 501 CGCTTCAGGA ACAGCTTCTG CTCCTGCCGA TAGTGCAGCT CCGGCAGAAG 551 CGAAGGCAGA AGACAAGGGT GCGGCAGCCC CTGCGGTCGG CGTTGACGGT 601 AAAAAAGTCT TCGAAGCAAC CTGTCAGGTG TGCCACGGCG GTTCGATTCC 651 CGGTATTCCC GGCATAGGCA AAAAAGACGA TTGGGCACCG CGTATCAAAA 701 AAGGCAAAGA AACCTTGCAC AAACACGCCC TTGAAGGCTT TAACGCGATG 751 CCTGCCAAAG GCGGCAATGC AGGTTTGAGC GATGACGAAG TCAAAGCGGC 801 TGTTGACTAT ATGGCAAACC AATCCGGTGC AAAATTCTAA This corresponds to the amino acid sequence <SEQ ID 504; ORF 130.a>: al30.pep 1 MKQLRDNKAQ GSALFTLVSG IVIVIAVLYF LIKLAGSGSF GDVDATTEAA 51 TQTRIQPVGQ LTMGDGIPVG ERQGEQIFGK ICIQCHAADS NVPNAPKLEH 101 NGDWAPRIAQ GFDTLFQHAL NGFNAMPAKG GAVDLTDQEL KRAITYMANK 151 SGGSFPNPDE AAPADNAASG TASAPADSAA PAEAKAEDKG AAAPAVGVDG 201 KKVFEATCQV CHGGSIPGIP GIGKKDDWAP RIKKGKETLH KHALEGFNAM 251 PAKGGNAGLS DDEVKAAVDY MANQSGAKF* ml30/al30 97.6% identity in 206 aa overlap 10 20 30 ml30.pep GEQIFGKICIQCHAADSNVPNAPKLEHNGD I I I I I I I I I I I I II I I I I I I I I I I I I I I I I al30 DATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNGD 50 60 70 80 90 100 40 50 60 70 80 89 ml30.pep XAPRI-QGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDEAAP I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al30 WAPRIAQGFDTLFQHALNGFNAMPAKGGAVDLTDQELKRAITYMANKSGGSFPNPDEAAP 110 120 130 140 150 160 90 100 110 120 130 140 ml30.pep ADNAASGTASAPADSAAPAEAKAEDKGAA-PAVGVDGKKVFEATCQVCHGGSIPGIPGIG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al30 ADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPGIPGIG 170 180 190 200 210 220 150 160 170 180 190 200 ml30. pep KKDDWAPRIKKGKETLHKHALEGFNAMPAKXGNAGLSDDEVKAAVDYMANQSGAKFX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al30 KKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSGAKFX 230 240 250 260 270 280 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 505>: gl32.seq 1 ATGGAAGCCT TCAAAACCCT AATTTGGATT ATTAATATTA TTTCCGCTTT 51 GGCCGTCATC GTGTTAGTAT TGCTCCAACA CGGCAAAGGC GCGGATGCCG 101 GCGCGACCTT CGGATCGGGA AGCGGCAGCG CGCAAGGCGT ATTCGGCTCT 151 GCCGGCAACG CCAACTTcct CAgccGCTCG AccGccGTTG CAGCAACAtt 201 tttcttTGca acctgcAtgg gctatggTgt atattcacac CCACACGACA 251 AAACACGGTT TGGACTtcag caacataCGA CAGACTCAGC AagcACCCAA 301 ACCcgtAAGC AATACCGAAC CTTCTGCCCC TGTTCCTCAG CAGCAGAAAT 351 AACagtTTTT CAAATgccga caTGgtga This corresponds to the amino acid sequence <SEQ ID 506; ORF 132.ng>: gl32.pep 1 MEAFKTLIWI IΝIISALAVI VLVLLQHGKG ADAGATFGSG SGSAQGVFGS 51 AGΝAΝFLSRS TAVAATFFFA TCMGYGVYSH PHDKTRFGLQ QHTTDSASTQ 101 TRKQYRTFCP CSSAAEITVF QMPTW* The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 507>: ml32.seq (partial) 1 ATGGAACCCT TCAAAACCTT AATTTGGATT GTTAATTTAA TTTCCGCTTT 51 GGCCGTCTTC GTGTTAGTAT TGCTCCAACA CGGCAAAGGC GCGGATGCCG 101 GCGCGACTTT CGGA... This corresponds to the amino acid sequence <SEQ ID 508; ORF 132>: ml32.pep (partial) 1 MEPFKTLIWI VΝLISALAVF VLVLLQHGKG ADAGATFG ... Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 132 shows 89.5% identity over a 38 aa overlap with a predicted ORF (ORF 132.ng) from N. gonorrhoeae: ml32/gl32 10 20 30 ml32.pep MEPFKTLIWIVΝLISALAVFVLVLLQHGKGADAGATFG I I M M M h h M M I h M M M M M I M M I M gl32 MEAFKTLIWIIΝIISALAVIVLVLLQHGKGADAGATFGSGSGSAQGVFGSAGΝAΝFLSRS 10 20 30 40 50 60 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 509>: al32. seq 1 ATGGAAGCCT TCAAAACCCT AATTTGGATT GTTAATATAA TTTCCGCTTT 51 GGCCGTCATC GTGTTAGTAT TGCTCCAACA CGGCAAAGGC GCGGATGCCG 101 GCGCGACTTT CGGATCGGGA AGCGGCAGCG CGCAAGGCGT ATTCGGCTCT 151 GCCGGCAACG CTAACTTCCT CAGCCGCTCG ACCGCCGTTG CAGCAACATT 201 TTTCTTTGCA ACCTGCATGg GCTATGGTGT ATATTCACAC CCACACGACA 251 AAACACGGTT TGGACTTCAG CAACGTACAA CAAACTCAGC AAGCACCCAA 301 ACCCGTAAGC AATACCGAAC CTTCTGCCCC TGTTCCTCAG CAGCAGAAAT 351 AACAGTTTTT CAAATGCCGA CATGGTGA This corresponds to the amino acid sequence <SEQ ID 510; ORF 132. a>: al32.pep 1 MEAFKTLIWI VΝIISALAVI VLVLLQHGKG ADAGATFGSG SGSAQGVFGS 51 AGΝAΝFLSRS TAVAATFFFA TCMGYGVYSH PHDKTRFGLQ QRTTΝSASTQ 101 TRKQYRTFCP CSSAAEITVF QMPTW* ml32/al32 92.1% identity in 38 aa overlap 10 20 30 ml32. pep MEPFKTLIWIVNLISALAVFVLVLLQHGKGADAGATFG II I I I I I I I I I : I I I I I I : I I I I I I I I I I I I I I I I I I al32 MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSAQGVFGSAGNANFLSRS 10 20 30 40 50 60 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 511>: gl34. seq 1 ATGTCCCAAG AAATCCTCGA CCAAGTGCGC CGCCGCCGCA CGTTTGCCAT 51 CATCTCCCAC CCCGATGCGG GTAAAACCAC GCTGACCGAA AAACTGCTGC 101 TGTTTTCGGG CGCGATTCAA AGCGCAGGCA CGGTGAAAGG TAAGAAAACC 151 GGCAAATTCG CCACCTCCGA CTGGATGGAC ATCGAGAAGC AGCGCGGCAT 201 TTCCGTGGCA TCAAGCGTGA TGCAGTTCGA CTACAAAGAC CACACCGTCA 251 ACCTCTTGGA CACGCCGGGA CACCAAGACT TCTCCGAAGA CACCTACCGC 301 GTTTTAACCG CAGTGGACAG CGCCTTGATG GTCATCGACG CGGCAAAAGG 351 CGTGGAAGCG CAAACCATCA AACTCTTGAA CGTCTGCCGC CTGCGCGATA 401 CGCCGATTGT TACCTTCATG AACAAATACG ACCGCGAAGT GCGCGATTCT 451 TTGGAACTCT TGGACGAAGT GGAAGACATC CTGCAAATCC GCTGCGCGCC 501 CGTTACCTGG CCGATCGGTA TGGGCAAAAA CTTCAAGGGC GTGTACCACA 551 TCCTGAACGA CGAAATCTAT CTCTTTGAAG CGGGCGGCGA ACGCCTGCCG 601 CACGAGTTCG ACATCATCAA AGGCATAAAC AATCCCGAAT TGGAACAACG 651 CTTTCCGTTG GAAATCCAGC AGTTGCGCGA CGAAATCGAA TTGGTGCAGG 701 CGGCTTCCAA CGAATTTAAT CTCGacgaAT TTCTCGccgG CGAACTCACG 751 CCAGTGTTCT TCGGCTCTGC GATTAACAAC TTCGGCATTC AGGAAATCCT 801 CAATTCATTG ATTGACTGGG CACCCGCACC GAAACCGCGC GACGCGACCA 851 TGCGCATGGT CGGGCCGGAC GAGCCGAAAT TTTCCGGATT TATCTTTAAA 901 ATCCAAGCCA ATATGGACCC GAAACACCGC GACCGTATCG CCTTCTTGCG 951 CGTCTGCTCC GGTAAATTCG AGCGCGGCAT GAAGATGAAA CACCTGCGTA 1001 TCAACCGCGA AATCGCCGCC TCCAGCGTAG TAACCTTCAT GTCGCACGAC 1051 CGCGAACTGG CGGAAGAAGC CTACGCCGGC GACATCATCG GCATCCCGAA 1101 CCACGGCAAC ATCCAAATCG GCGACAGCTT CTCCGAAGGC GAACAACTGG 1151 CGTTTACCGG CATCCCATTC TTCGCGCCCG AACTGTTCCG CAGCGTCCGC 1201 ATCAAAAACC CGCTGAAAAT CAAACAACTG CAAAAAGGTT TGCAACAACT 1251 CGGCGAAGAA GGTGCGGTTC AAGTATTCAA ACCGATGAGC GGCGCGGATT 1301 TGATTTTGGG TGCGGTCGGC GTGTTGCAGT TTGAAGTCGT AACCTCACGC 1351 CTCGCCAACG AATACGGCGT GGAAGCCGTG TTCGACAGCG CATCCATCTG 1401 GTCGGCGCGC TGGGTATCGT GCGACGACAA GAAAAAACTG GCGGAATTTG 1451 AAAAAGCCAA CGCAGGCAAC CTCGCCATCG ACGCAGGCGG CAACCTCGCC 1501 TACCTCGCCC CCAACCGCGT GAATTTGGGG TTGACGCAAG AACGCTGGCC 1551 GGACATCGTG TTCCACGAAA CGCGCGAACA TTCGGTCAAA CTCTAA This corresponds to the amino acid sequence <SEQ ID 512; ORF 134.ng>: gl34.pep 1 MSQEILDQVR RRRTFAIISH PDAGKTTLTE KLLLFSGAIQ SAGTVKGKKT 51 GKFATSDWMD IEKQRGISVA SSVMQFDYKD HTVΝLLDTPG HQDFSEDTYR 101 VLTAVDSALM VIDAAKGVEA QTIKLLΝVCR LRDTPIVTFM ΝKYDREVRDS 151 LELLDEVEDI LQIRCAPVTW PIGMGKΝFKG VYHILΝDEIY LFEAGGERLP 201 HEFDIIKGIΝ ΝPELEQRFPL EIQQLRDEIE LVQAASΝEFΝ LDEFLAGELT 251 PVFFGSAIΝΝ FGIQEILΝSL IDWAPAPKPR DATMRMVGPD EPKFSGFIFK 301 IQAΝMDPKHR DRIAFLRVCS GKFERGMKMK HLRIΝREIAA SSWTFMSHD 351 RELAEEAYAG DIIGIPΝHGΝ IQIGDSFSEG EQLAFTGIPF FAPELFRSVR 401 IKΝPLKIKQL QKGLQQLGEE GAVQVFKPMS GADLILGAVG VLQFEWTSR 451 LAΝEYGVEAV FDSASIWSAR WVSCDDKKKL AEFEKAΝAGΝ LAIDAGGΝLA 501 YLAPΝRVΝLG LTQERWPDIV FHETREHSVK L* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 513>: ml34. seq 1 ATGTCCCAAG AAATCCTCGA CCAAGTGCGC CGCCGCCGCA CGTTTGCCAT 51 CATCTCCCAC CCTGACGCAG GTAAAACCAC GTTGACTGAA AAACTCTTGC 101 TGTTTTCGGG CGCGATTCAG AGCGCGGGTA CGGTAAAAGG CAAGAAAACC 151 GGCAAATTCG CCACTTCCGA CTGGATGGAA ATCGAGAAGC AGCGCGGCAT 201 TTCCGTGGCA TCAAGTGTGA TGCAGTTCGA TTACAAAGAC CACACCGTCA 251 ACCTCTTGGA CACGCCGGGA CACCAAGACT TCTCCGAAGA CACCTACCGC 301 GTTTTAACCG CCGTGGACAG CGCATTAATG GTCATCGACG CGGCAAAAGG 351 CGTGGAAGCG CAAACCATCA AGCTCTTAAA CGTCTGCCGC CTGCGCGATA 401 CACCGATTGT TACGTTTATG AACAAATACG ACCGCGAAGT GCGCGATTCC 451 CTGGAACTTT TGGACGAAGT GGAAAACATT TTAAAAATCC GCTGCGCGCC 501 CGTTACCTGG CCGATCGGTA TGGGCAAAAA CTTCAAGGGC GTGTACCACA 551 TCCTGAACGA TGAAATTTAT CTCTTTGAAG CTGGCGGCGA ACGCCTGCCG 601 CACGAGTTCG ACATCATCAA AGGCATCGAT AATCCTGAAT TGGAACAACG 651 CTTTCCGTTG GAAATCCAGC AGTTGCGCGA CGAAATCGAA TTGGTGCAGG 701 CGGCTTCCAA CGAGTTTAAT CTCGACGAAT TCCTCGCCGG CGAACTCACG 751 CCCGTATTCT TCGGCTCTGC GATTAACAAC TTCGGTATTC AGGAAATCCT 801 CAATTCATTG ATTGACTGGG CGCCCGCGCC GAAACCGCGC GACGCGACCG 851 TACGTATGGT CGAGCCGGAC GAGCCGAAGT TTTCCGGATT TATCTTCAAA 901 ATCCAAGCCA ATATGGACCC GAAACACCGC GACCGTATTG CCTTCTTGCG 951 CGTCTGCTCC GGCAAATTCG AGCGCGGCAT GAAGATGAAA CACCTGCGTA 1001 TCAACCGCGA AATCGCCGCC TCCAGCGTGG TTACCTTCAT GTCGCACGAC 1051 CGCGAGCTGG TTGAAGAAGC CTACGCCGGC GACATTATCG GCATCCCGAA 1101 CCACGGCAAC ATCCAAATCG GCGACAGCTT CTCCGAAGGC GAACAACTGG 1151 CGTTCACCGG CATCCCATTC TTCGCACCCG AACTGTTCCG CAGCGTACGC 1201 ATCAAAAACC CGCTGAAAAT CAAACAACTG CAAAAAGGCT TGCAACAGCT 1251 CGGCGAAGAA GGCGCGGTGC AGGTGTTCAA ACCGATGAGC GGCGCGGATT 1301 TGATTTTGGG CGCGGTCGGC GTGTTGCAGT TTGAAGTCGT TACCTCGCGC 1351 CTCGCCAACG AATACGGCGT AGAAGCCGTG TTCGACAGCG CATCCATCTG 1401 GTCGGCGCGC TGGGTATCGT GCGACGACAA GAAAAAACTG GCTGAATTTG 1451 AAAAAGCCAA CGCGGGCAAC CTCGCCATCG ACGCAGGCGG CAACCTCGCC 1501 TACCTCGCCC CCAACCGCGT GAATTTGGGA CTCACGCAAG AACGTTGGCC 1551 GGACATCGTG TTCCACGAAA CACGCGAACA TTCGGTCAAA CTGTAA This corresponds to the amino acid sequence <SEQ ID 514; ORF 134>: ml34.pep 1 MSQEILDQVR RRRTFAIISH PDAGKTTLTE KLLLFSGAIQ SAGTVKGKKT 51 GKFATSDWME IEKQRGISVA SSVMQFDYKD HTVNLLDTPG HQDFSEDTYR 101 VLTAVDSALM VIDAAKGVEA QTIKLLNVCR LRDTPIVTFM NKYDREVRDS 151 LELLDEVENI LKIRCAPVTW PIGMGKNFKG VYHILNDEIY LFEAGGERLP 201 HEFDIIKGID NPELEQRFPL EIQQLRDEIE LVQAASNEFN LDEFLAGELT 251 PVFFGSAINN FGIQEILNSL IDWAPAPKPR DATVRMVEPD EPKFSGFIFK 301 IQANMDPKHR DRIAFLRVCS GKFERGMKMK HLRINREIAA SSWTFMSHD 351 RELVEEAYAG DIIGIPNHGN IQIGDSFSEG EQLAFTGIPF FAPELFRSVR 401 IKNPLKIKQL QKGLQQLGEE GAVQVFKPMS GADLILGAVG VLQFEWTSR 451 LANEYGVEAV FDSASIWSAR WVSCDDKKKL AEFEKANAGN LAIDAGGNLA 501 YLAPNRVNLG LTQERWPDIV FHETREHSVK L* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N. gonorrhoeae ORF 134 shows 98.7% identity over a 531 aa overlap with a predicted ORF (ORF 134.ng) from N gonorrhoeae: ml34/gl34 10 20 30 40 50 60 ml34.pep MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGTVKGKKTGKFATSDWME 1111111111 E 11111 ! 11111111111 i 1111111 M 111111111 ! i 11 ! 1111111 : gl34 MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGTVKGKKTGKFATSDWMD 10 20 30 40 50 60 70 80 90 100 110 120 ml34.pep IEKQRGISVASSVMQFDYKDHTVΝLLDTPGHQDFSEDTYRVLTAVDSALMVIDAAKGVEA M M M M M I M I M M M M M M M I M M M I M M M M M I M M I M M M M gl34 IEKQRGISVASSVMQFDYKDHTVΝLLDTPGHQDFSEDTYRVLTAVDSALMVIDAAKGVEA 70 80 90 100 110 120 130 140 150 160 170 180 ml34.pep QTIKLLNVCRLRDTPIVTFMNKYDREVKDSLELLDEVENILKIRCAPVTWPIGMGKNFKG gl34 QTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVEDILQIRCAPVTWPIGMGKNFKG 130 140 150 160 170 180 190 200 210 220 230 240 ml34.pep VYHILNDEIYLFEAGGERLPHEFDIIKGIDNPELEQRFPLEIQQLRDEIELVQAASNEFN gl34 VYHILNDEIYLFEAGGERLPHEFDIIKGINNPELEQRFPLEIQQLRDEIELVQAASNEFN 190 200 210 220 230 240 250 260 270 280 290 300 ml34. pep LDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPAPKPRDATVRMVEPDEPKFSGFIFK MMMMMMIMMMIMMMMMMMMMMIIhMI 111111111111 gl34 LDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPAPKPRDATMRMVGPDEPKFSGFIFK 250 260 270 280 290 300 310 320 330 340 350 360 ml34.pep IQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRINREIAASSWTFMSHDRELVEEAYAG MMMMMIMMMIMMMMM I II 1111 II II II I II I h II II I I gl34 IQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRINREIAASSWTFMSHDRELAEEAYAG 310 320 330 340 350 360 370 380 390 400 410 420 ml34.pep DIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFAPELFRSVRIKNPLKIKQLQKGLQQLGEE MMMMMMMMMMIMMMMMMMMMMMMMMMMMMMI gl34 DIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFAPELFRSVRIKNPLKIKQLQKGLQQLGEE 370 380 390 400 410 420 430 440 450 460 470 480 ml34.pep GAVQVFKPMSGADLILGAVGVLQFEWTSRLANEYGVEAVFDSASIWSARWVSCDDKKKL MMMMMIMMMIMMMMMIMMMMIMMMMMMMMMMM gl34 GAVQVFKPMSGADLILGAVGVLQFEWTSRLANEYGVEAVFDSASIWSARWVSCDDKKKL 430 440 450 460 470 480 490 500 510 520 530 ml34.pep AEFEKANAGNLAIDAGGNLAYLAPNRVNLGLTQERWPDIVFHETREHSVKLX IMMMMMMMMMMIMMMMMMMMMIMIMMMM gl34 AEFEKANAGNLAIDAGGNLAYLAPNRVNLGLTQERWPDIVFHETREHSVKLX 490 500 510 520 530 The following partial DNA sequence was identified in N meningitidis <SEQ ID 515>: al3 .seq 1 ATGTCCCAAG AAATCCTCGA CCAAGTGCGC CGCCGCCGCA CGTTTGCCAT 51 CATCTCCCAC CCTGACGCAG GTAAAACCAC GTTGACTGAA AAACTCTTGC 101 TGTTTTCAGG TGCGATTCAA AGCGCGGGTA CGGTAAAAGG CAAGAAAACC 151 GGCAAATTCG CCACCTCCGA CTGGATGGAC ATCGAGAAGC AGCGCGGCAT 201 TTCCGTGGCA TCAAGCGTGA TGCAGTTCGA CTATAAAGAC CACACCGTCA 251 ACCTTTTGGA CACGCCGGGA CACCAAGACT TCTCCGAAGA CACCTACCGC 301 GTTTTGACCG CCGTCGATAG TGCCTTGATG GTCATCGACG CGGCAAAAGG 351 CGTGGAAGCG CAAACCATCA AACTCTTGAA CGTCTGCCGC CTGCGCAATA 401 CGCCGATTGT TACGTTCATG AACAAATACG ACCGCGAAGT GCGCGATTCC 451 CTGGAATTGC TGGACGAAGT GGAAAACATC CTGCAAATCC GCTGCGCGCC 501 CGTAACCTGG CCGATCGGCA TGGGCAAAAA CTTCAAAGGC GTGTACCACA 551 TCCTGAACGA CGAAATCTAT CTCTTTGAAG CGGGCGGCGA ACGCTTGCCG 601 CACGAGTTCG ACATCATCAA AGGCATCGAT AATCCCGAAT TGGAACAACG 651 CTTTCCGTTA GAAATACAGC AGTTGCGCGA CGAAATCGAA TTGGTGCAGG 701 CGGCTTCCAA CGAGTTCAAT CTCGACGAAT TCCTCGCCGG CGAACTCACG 751 CCCGTATTCT TCGGCTCTGC GATTAACAAC TTCGGTATTC AGGAAATCCT 801 CAATTCATTG ATTGAATGGG CGCCCGCGCC GAAACCACGC GATGCGACCG 851 TGCGTATGGT CGAGCCGGAC GAGCCGAAGT TTTCCGGATT TATCTTCAAA 901 ATCCAAGCCA ATATGGACCC GAAACACCGC GACCGTATTG CCTTCTTGCG 951 CGTCTGCTCC GGCAAATTCG AGCGCGGCAT GAAAATGAAA CACCTGCGTA 1001 TCAACCGCGA AATCGCCGCC TCCAGCGTGG TAACCTTCAT GTCCCACGAC 1051 CGCGAGCTGG TTGAAGAAGC CTACGCCGGC GACATTATCG GTATCCCAAA 1101 CCACGGCAAC ATCCAAATCG GCGACAGCTT CTCCGAAGGC GAACAACTGA 1151 CGTTTACCGG CATCCCATTC TTCGCGCCCG AACTGTTCCG CAGCGTTCGC 1201 ATCAAAAACC CGCTGAAAAT CAAGCAACTG CAAAAAGGTT TGCAACAGCT 1251 TGGCGAAGAA GGTGCGGTGC AGGTGTTCAA ACCAATGAGC GGCGCGGATT 1301 TGATTTTGGG CGCGGTCGGC GTGTTGCAGT TTGAAGTCGT TACCTCGCGC 1351 CTTGCCAACG AATACGGCGT GGAAGCCGTG TTCGACAACG CATCCATCTG 1401 GTCGGCGCGC TGGGTATCGT GCGACGACAA GAAAAAACTG GCGGAATTTG 1451 AAAAAGCCAA CGCGGGCAAC CTCGCCATCG ACGCGGGCGG CAACCTCGCC 1501 TACCTCGCCC CTAACCGCGT GAATCTGGGA CTCACGCAAG AACGCTGGCC 1551 GGACATCGTG TTCCACGAAA CGCGCGAGCA TTCGGTCAAA CTTTAA This corresponds to the amino acid sequence <SEQ ID 516; ORF 134.a>: al34.pep 1 MSQEILDQVR RRRTFAIISH PDAGKTTLTE KLLLFSGAIQ SAGTVKGKKT 51 GKFATSDWMD IEKQRGISVA SSVMQFDYKD HTVNLLDTPG HQDFSEDTYR 101 VLTAVDSALM VIDAAKGVEA QTIKLLNVCR LRNTPIVTFM NKYDREVRDS 151 LELLDEVENI LQIRCAPVTW PIGMGKNFKG VYHILNDEIY LFEAGGERLP 201 HEFDIIKGID NPELEQRFPL EIQQLRDEIE LVQAASNEFN LDEFLAGELT 251 PVFFGSAINN FGIQEILNSL IEWAPAPKPR DATVRMVEPD EPKFSGFIFK 301 IQANMDPKHR DRIAFLRVCS GKFERGMKMK HLRINREIAA SSWTFMSHD 351 RELVEEAYAG DIIGIPNHGN IQIGDSFSEG EQLTFTGIPF FAPELFRSVR 401 IKNPLKIKQL QKGLQQLGEE GAVQVFKPMS GADLILGAVG VLQFEWTSR 451 LANEYGVEAV FDNASIWSAR WVSCDDKKKL AEFEKANAGN LAIDAGGNLA 501 YLAPNRVNLG LTQERWPDIV FHETREHSVK L* ml34/al34 98.9% identity in 531 aaoverlap 10 20 30 40 50 60 ml34..pep MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGTVKGKKTGKFATSDWME I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : al3 MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGTVKGKKTGKFATSDWMD 10 20 30 40 50 60 70 80 90 100 110 120 ml34. pep IEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVLTAVDSALMVIDAAKGVEA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al34 IEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVLTAVDSALMVIDAAKGVEA 70 80 90 100 110 120 130 140 150 160 170 180 ml34.pep QTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVENILKIRCAPVTWPIGMGKNFKG I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I: I I I I I I I I II I I I I I I I I al34 QTIKLLNVCRLRNTPIVTFMNKYDREVRDSLELLDEVENILQIRCAPVTWPIGMGKNFKG 130 140 150 160 170 180 190 200 210 220 230 240 ml34. pep VYHILNDEIYLFEAGGERLPHEFDIIKGIDNPELEQRFPLEIQQLRDEIELVQAASNEFN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al34 VYHILNDEIYLFEAGGERLPHEFDIIKGIDNPELEQRFPLEIQQLRDEIELVQAASNEFN 190 200 210 220 ,230 240 250 260 270 280 290 300 ml34.pep LDEFLAGELTPVFFGSAINNFGIQEILNSLIDWAPAPKPRDATVRMVEPDEPKFSGFIFK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I: I I II I I I I I I I I I I I I I I I I I II I I I I I al34 LDEFLAGELTPVFFGSAINNFGIQEILNSLIEWAPAPKPRDATVRMVEPDEPKFSGFIFK 250 260 270 280 290 300 310 320 330 340 350 360 ml34. pep IQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRINREIAASSWTFMSHDRELVEEAYAG II I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I al34 I Q ANMDPKHRDRIAFLRVCSGKFERGMKMKHLRINREIAASSWTFMSHDRELVEEAYAG 310 320 330 340 350 360 370 380 390 400 410 420 ml3 .pep DIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFAPELFRSVRIKNPLKIKQLQKGLQQLGEE II I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al34 DI IGIPNHGNIQIGDSFSEGEQLTFTGIPFFAPELFRSVRIKNPLKIKQLQKGLQQLGEE 370 380 390 400 410 420 430 440 450 460 470 480 ml34 . pep GAVQVFKPMSGADLILGAVGVLQFEWTSRLANEYGVEAVFDSASIWSARWVSCDDKKKL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I al34 GAVQVFKPMSGADLILGAVGVLQFEWTΞRLANEYGVEAVFDNASIWSARWVSCDDKKKL 430 440 450 460 470 480 490 500 510 520 530 ml34.pep AEFEKANAGNLAIDAGGNLAYLAPNRVNLGLTQERWPDIVFHETREHSVKLX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I al34 AEFEKANAGNLAIDAGGNLAYLAPNRVNLGLTQERWPDIVFHETREHSVKLX 490 500 510 520 530 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 517>: gl35.seq 1 ATGAAATACA AAAGAATCGT ATTTAAAGTC GGCACATCTT CGATTACCCG 51 TTCGGAcgGC AGCCTCTCGC GCGGCAAAAT CCAAACCATC ACCCGCCAGC 101 TTGCCGCATT GCATCATGCG GGACACGAGC TGGTCTTGGT GTCTTCCGGC 151 GCGGTTGCTG CAGGGTTCGG CGCGCTGGGT TTCAAAAAAC GTCCGGTCAA 201 AATCGCCGAC AAACAGGCTT CCGCCGCCGT CGGGCAGGGG CTGCTGATGG 251 AAGAATATAC GGCAAACCTG TCTTCAGACG GCATCGTGTC CGCACAAATC 301 CTGCTCAGCC GTGCCGACTT TGCCGACAAA CGCCGCTACC AAAATGCCGG 351 CGGCGCACTT TCCGTGCTGC TGCAACGCCG CGCGATTCCC ATCATCAATG 401 AAAACGACAC GGTTTCGGTT GAGGAGTTGA AAATCGGCGA CAACGACACA 451 TTGAGTGCGC AAGTGGCGGC GATGATACAG GCAGACCTCT TGGTGCTGCT 501 GACCGACATA GACGGTCTTT ACACCGGCAA CCCGAACAGC AATCCCGATG 551 CCGTACGGCT GGACAAAATC GAACACATCA ACCATGAAAT CATCGAAATG 601 GCGGGCGGCT CGGGTTCGGC AAACGGCACG GGCGGTATGC TGACCAAAAT 651 CAAAGCGGCA ACCATCGCCG CCGAATCCGG CGTACCGGTG TATATCTGTT 701 CCTCACTCAA ACCCGATTCA TTGGCCGAAG CCGCCGAACA TCAGGCGGAC 751 GGCTCGTTTT TCGTcccCcg tgCCAAAGGT TTGCGGACAC AGAAGCAATG 801 GctggCGTTC TATTCcgaaa gcggGGgcag cgttTAtgtg gacgaaagtg 851 cggaacacgc tTtgtccgaa caagggaaag cctgCTGA This corresponds to the amino acid sequence <SEQ ID 518; ORF 135.ng>: gl35.pep 1 MKYKRIVFKV GTSSITRSDG SLSRGKIQTI TRQLAALHHA GHELVLVSSG 51 AVAAGFGALG FKKRPVKIAD KQASAAVGQG LLMEEYTAΝL SSDGIVSAQI 101 LLSRADFADK RRYQΝAGGAL SVLLQRRAIP IIΝEΝDTVSV EELKIGDΝDT 151 LSAQVAAMIQ ADLLVLLTDI DGLYTGΝPΝS ΝPDAVRLDKI EHIΝHEIIEM 201 AGGSGSAΝGT GGMLTKIKAA TIAAESGVPV YICSSLKPDS LAEAAEHQAD 251 GSFFVPRAKG LRTQKQWLAF YSESGGSVYV DESAEHALSE QGKAC* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 519>: ml35. seq 1 ATGAAATACA AAAGAATCGT ATTTAAAGTC GGCACATCTT CGATTACCCA 51 TTCGGACGGC AGTCTCTCGC GCGGCAAAAT CCAAACCATC ACCTGCCAGC 101 TTGCCGCATT GCATCATGCG GGACACGAGC TGGTCTTGGT GTCTTCCGGC 151 GCGGTTGCGG CAGGGTTCGG TGCGCTGGGT TTCAAAAAAC GTCCGGTCAA 201 AATCGCCGAC AAACAGGCTT CCGCCGCCGT CGGGCAGGGG CTGCTGATGG 251 AAGAATATAC GGCAAACCTG TCTTCAGACG GCATCGTGTC CGCGCAAATC 301 CTGCTCAGCC GCGCCGACTT TGCCGACAAA CGCCGCTACC AAAATGCCGG 351 CGGCGCACTT TCCGTGCTGC TGCAACGCCG CGCCGTCCCC ATCATCAATG 401 AAAACGATAC GGTTTCGGTT GAGGAATTGA AAATCGGCGA CAACGACACA 451 TTGAGTGCGC AAGTGGCGGC GATGATACAG GCAGACCTCT TGGTGCTGCT 501 GACCGACATA GACGGTCTTT ACACGGGCAA CCCGAACAGC AATCCCGATG 551 CCGTACGGCT GGACAAAATC GAACACATCA ACCATGAAAT CATCGAAATG 601 GCGGGCGGCT CGGGTTCGGC AAACGGCACG GGCGGTATGC TGACCAAAAT 651 CAAAGCGGCA ACCATCGCCG CCGAATCCGG CGTACCGGTG TATATCTGTT 701 CCTCGCTCAA ACCCGATGCA CTTGCCGAAG CTGCCGAACA TCAGGCGGAC 751 GGCTCGTTTT TCGTCCCCCG TGCCAAAGGT TTGCGGACGC AGAAGCAATG 801 GCTGGCGTTC TATTCCGAAA GCCGGGGCAG CGTTTATGTG GACGAAGGTG 851 CGGAACACGC TTTGTCCGAA CAGGGGAAAA GCCTGCTGAT GTCGGGCATT 901 GCCGGAATCG AAGGGCATTT TTCCCGTATG GACACCGTAA CCGTGTACAG 951 CAAGGCAACC AAACAGCCCC TGGGCAAAGG GCGCGTCCTG TTCGGCTCTG 1001 CCGCCGCCGA AGACCTGCTC AAATCGCGTA AGGCGAAAGG CGTGTTCATC 1051 CATCGGGACG ACTGGATTTC CATCACGCCC GAAATACGCC TGCTTCTGAC 1101 CGAATTTTAG This corresponds to the amino acid sequence <SEQ ID 520; ORF 135>: ml35.pep 1 MKYKRIVFKV GTSSITHSDG SLSRGKIQTI TCQLAALHHA GHELVLVSSG 51 AVAAGFGALG FKKRPVKIAD KQASAAVGQG LLMEEYTANL SSDGIVSAQI 101 LLSRADFADK RRYQNAGGAL SVLLQRRAVP IINENDTVSV EELKIGDNDT 151 LSAQVAAMIQ ADLLVLLTDI DGLYTGNPNS NPDAVRLDKI EHINHEIIEM 201 AGGSGSANGT GGMLTKIKAA TIAAESGVPV YICSSLKPDA LAEAAEHQAD 251 GSFFVPRAKG LRTQKQWLAF YSESRGSVYV DEGAEHALSE QGKSLLMSGI 301 AGIEGHFSRM DTVTVYSKAT KQPLGKGRVL FGSAAAEDLL KSRKAKGVFI 351 HRDDWISITP EIRLLLTEF* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 135 shows 97.6% identity over a 294 aa overlap with a predicted ORF (ORF 135.ng) from N gonorrhoeae: ml35/gl35 10 20 30 40 50 60 ml35.pep MKYKRIVFKVGTSSITHSDGSLSRGKIQTITCQLAALHHAGHELVLVSSGAVAAGFGALG MMIMMIMIMhMMMMMMM I II II 111 II 1111111 II II 111 II 11 gl35 MKYKRIVFKVGTSSITRSDGSLSRGKIQTITRQLAALHHAGHELVLVSSGAVAAGFGALG 10 20 30 40 50 60 70 80 90 100 110 120 ml35. pep FKKRPVKIADKQASAAVGQGLLMEEYTAΝLSSDGIVSAQILLSRADFADKRRYQΝAGGAL MMMMMIMMMMMMMMMMMMMMMMMMMIMIMIMM gl35 FKKRPVKIADKQASAAVGQGLLMEEYTAΝLSSDGIVSAQILLSRADFADKRRYQΝAGGAL 70 80 90 100 110 120 130 140 150 160 170 180 ml35.pep SVLLQRRAVPIIΝEΝDTVSVEELKIGDΝDTLSAQVAAMIQADLLVLLTDIDGLYTGΝPΝS gl35 SVLLQRRAIPIIΝEΝDTVSVEELKIGDΝDTLSAQVAAMIQADLLVLLTDIDGLYTGΝPΝS 130 140 150 160 170 180 190 200 210 220 230 240 ml35.pep ΝPDAVRLDKIEHIΝHEIIEMAGGSGSAΝGTGGMLTKIKAATIAAESGVPVYICSSLKPDA MMMMMMIIMMMIMMMMMMMMMMMMMIMIMMMIh gl35 ΝPDAVRLDKIEHIΝHEIIEMAGGSGSAΝGTGGMLTKIKAATIAAESGVPVYICSSLKPDS 190 200 210 220 230 240 250 260 270 280 290 300 ml35. pep LAEAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRGSVYVDEGAEHALSEQGKSLLMSGI MMMMMMMMMMIMMIMIMMI MMIIhllMMMIh gl35 LAEAAEHQADGSFFVPRAKGLRTQKQWLAFYSESGGSVYVDESAEHALSEQGKACX 250 260 270 280 290 310 320 330 340 350 360 ml35 . pep AGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSAAAEDLLKSRKAKGVFIHRDDWISITP The following partial DNA sequence was identified in N meningitidis <SEQ ID 521>: al35. seq 1 ATGAAATACA AAAGAATCGT ATTTAAAGTC GGCACATCTT CGATTACCCA 51 TTCGGACGGC AGTCTCTCGC GCGGCAAAAT CCAAACCATC ACCCGCCAGC 101 TTGCCGCATT GCATCATGCG GGACACGAGC TGGTCTTGGT GTCTTCCGGC 151 GCGGTTGCGG CAGGGTTCGG TGCGCTGGGT TTCAAAAAAC GTCCGGTCAA 201 AATCGCCGAC AAACAGGCTT CCGCCGCCGT CGGGCAGGGG CTGCTGATGG 251 AAGAATATAC GGCAAACCTG TCTTCAGACG GCATCGTGTC CGCACAAATC 301 CTGCTCAGCC GCGCCGACTT TGCCGACAAA CGCCGCTACC AAAATGCCGG 351 CGGCGCACTT TCCGTGCTGC TGCAACGCCG CGCCGTCCCC ATCATCAATG 401 AAAACGATAC GGTTTCGGTT GAGGAATTGA AAATCGGCGA CAACGACACA 451 TTGAGTGCGC AAGTGGCGGC GATGATACAG GCAGACCTCT TGGTGCTGCT 501 GACCGACATA GACGGTCTTT ACACCGGCAA CCCGAACAGC AATCCCGATG 551 CCGTACGGCT GGACAAAATC GAACACATCA ACCATGAAAT CATCGAAATG 601 GCGGGCGGCT CGGGTTCGGC AAACGGCACA GGCGGTATGC TGACTAAAAT 651 CAAAGCGGCG ACGATTGCGA CCGAGTCCGG CGTACCGGTC TATATCTGTT 701 CCTCGCTCAA ACCCGATGCA CTTGCCGAAG CGGCAGATAA TCAGGCGGAC 751 GGCTCGTTTT TCGTCCCCCG TGCCAAAGGT TTGCGGACGC AGAAGCAATG 801 GCTGGCGTTC TATTCCGAAA GCAGGGGCGG CGTTTATGTG GACGAAGGTG 851 CGGAACACGC TTTGTCCGAA CAGGGAAAAA GCCTGCTGAT GTCGGGCATT 901 GCCGGAATCG AAGGGCATTT TTCCCGTATG GACACCGTAA CCGTGTACAG 951 CAAGGCAACC AAACAGCCTT TGGGCAAAGG GCGAGTCCTG TTCGGCTCTG 1001 CCGCCGCCGA AGACCTGCTC AAATTGCGTA AGGCGAAAGG CGTGTTCATC 1051 CATCGGGACG ACTGGATTTC CATCACGCCC GAAATACGCC TGCTTCTGAC 1101 CGAATTTTAG This corresponds to the amino acid sequence <SEQ ID 522; ORF 135. a>: al35.pep 1 MKYKRIVFKV GTSSITHSDG SLSRGKIQTI TRQLAALHHA GHELVLVSSG 51 AVAAGFGALG FKKRPVKIAD KQASAAVGQG LLMEEYTAΝL SSDGIVSAQI 101 LLSRADFADK RRYQΝAGGAL SVLLQRRAVP IIΝEΝDTVSV EELKIGDΝDT 151 LSAQVAAMIQ ADLLVLLTDI DGLYTGΝPΝS ΝPDAVRLDKI EHIΝHEIIEM 201 AGGSGSAΝGT GGMLTKIKAA TIATESGVPV YICSSLKPDA LAEAADΝQAD 251 GSFFVPRAKG LRTQKQWLAF YSESRGGVYV DEGAEHALSE QGKSLLMSGI 301 AGIEGHFSRM DTVTVYSKAT KQPLGKGRVL FGSAAAEDLL KLRKAKGVFI 351 HRDDWISITP EIRLLLTEF* ml35/al35 98.4% identity in 369 aa overlap 10 20 30 40 50 60 ml35.pep MKYKRIVFKVGTSSITHSDGSLSRGKIQTITCQLAALHHAGHELVLVSSGAVAAGFGALG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al35 MKYKRIVFKVGTSSITHSDGSLSRGKIQTITRQLAALHHAGHELVLVSSGAVAAGFGALG 10 20 30 40 50 60 70 80 90 100 110 120 ml35. pep FKKRPVKIADKQASAAVGQGLLMEEYTAΝLSSDGIVSAQILLSRADFADKRRYQΝAGGAL I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al35 FKKRPVKIADKQASAAVGQGLLMEEYTAΝLSSDGIVSAQILLSRADFADKRRYQΝAGGAL 70 80 90 100 110 120 130 140 150 160 170 180 ml35.pep SVLLQRRAVPIIΝEΝDTVSVEELKIGDΝDTLSAQVAAMIQADLLVLLTDIDGLYTGΝPΝS I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al35 SVLLQRRAVPIIΝEΝDTVSVEELKIGDΝDTLSAQVAAMIQADLLVLLTDIDGLYTGΝPΝS 130 140 150 160 170 180 190 200 210 220 230 240 ml35. pep ΝPDAVRLDKIEHIΝHEIIEMAGGSGSAΝGTGGMLTKIKAATIAAESGVPVYICSSLKPDA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I al35 ΝPDAVRLDKIEHIΝHEIIEMAGGSGSAΝGTGGMLTKIKAATIATESGVPVYICSSLKPDA 190 200 210 220 230 240 250 260 270 280 290 300 ml35. pep LAEAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRGSVYVDEGAEHALSEQGKSLLMSGI I I I I I :: I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I II I I I I I I I I I I I I I I I I I al35 LAEAADNQADGSFFVPRAKGLRTQKQWLAFYSESRGGVYVDEGAEHALSEQGKSLLMSGI 250 260 270 280 290 300 310 320 330 340 350 360 ml35.pep AGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSAAAEDLLKSRKAKGVFIHRDDWISITP I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al35 AGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSAAAEDLLKLRKAKGVFIHRDDWISITP 310 320 330 340 350 360 370 ml35. pep ElRLLLTEFX I I I I I I I I I I al35 EIRLLLTEFX 370 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 523>: gl36. seq 1 ATGGAAATCC GGTTTCAGAC AGCATTTTTA CGTTTGGTTC AGatgaAAAC 51 AAACGCTtca aTTCTtaccg caACACGCCT TGTATTTCCT GccgCTGCCG 101 CACGGACAGG GATCGTTCCT GCCGgtTTTT TCCCCTTCCC TGCGGACGGT 151 TTGCGGTTTG TTGATGACCG CCTGCCAGTA GCGGTAGATG TCtgccagcg 201 cgTAAGGCag tTCGGAcgca agttccgcca gctcgccttc ggTGAATTGC 251 AGgcggataa cgccgtttTC CTCTTCGTCg taaatgccgc ccactgccat 301 cacgGGGTAA AACAGCTCTT CAAACGCTTC ATCATCGGCG GCTTCAAACC 351 AATCGGTCGG CACAATGTCC AAACCGTAAA GATAGGCGTT GCACCAAGTG 401 TAAAAATCGC TGCCGCCCTC GCCGTCGTCG TAGAGCCACA AATCGGGCAG 451 CTTTTTATCC GACATCGCGG CGGTTGTTTC CATCGCCATT GCCAAAACCA 501 GCCGTTCGAT TTCGGAACGT TCGGCGGCGG TAAATTGCGA TTCGTCGCCC 551 AACACTTCGG GCAGCCAGTC GAGCGGTGCC AATTTGTCCG GCCCGCTCAA 601 CAGCGCCGTC ATAAAACCTT GAACCTCGTC GCAACGCATC GTGTTGCCTT 651 GTTCGCTTTT GGCATCCAAT AA This corresponds to the amino acid sequence <SEQ ID 524; ORF 136.ng>: gl36.pep 1 MEIRFQTAFL RLVQMKTΝAS ILTATRLVFP AAAARTGIVP AGFFPFPADG 51 LRFVDDRLPV AVDVCQRVRQ FGRKFRQLAF GELQADΝAVF LFWΝAAHCH 101 HGVKQLFKRF IIGGFKPIGR HΝVQTVKIGV APSVKIAAAL AVWEPQIGQ 151 LFIRHRGGCF HRHCQΝQPFD FGTFGGGKLR FVAQHFGQPV ERCQFVRPAQ 201 QRRHKTLΝLV ATHRVALFAF GIQ* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 525>: ml36.seq 1 ATGGAAACAA ACGCTTCAAT TCTTACCGCA ACACGCCTTG TATTTTCTGC 51 CGCTGCCGCA CGGACAGGGA TCGTTCCTGC CTGTTTTTTC GCCTTCCCTG 101 CGGACGGTTT GCGGTTTGTT GATGACTGCC TGCCAGTAGC GGTAGATATC 151 CGCCAATGCA TAAGGCAACT CGGATTCCAG TTCCGCCAGC TCGCCTTCTG 201 TGAATTGCAG ACGGATAGCG CCGTTTTCCT CTTCGTCGTA AATACCGCCC 251 AATGCCATGA TGGGATAAAA CAACTCTTCA AACGCTTCAT CATCGACGGC 301 TTCAAACCAA TCGGTCGGCA CAATATCCAA ACCGTAAAGA TAAGCATTGC 351 ACCATGTGTA AAAATCGCTG CCGCCGTCTT CGTTTTCATA CAGCCACAAA 401 TCGGGCAGTT TTTTATCCGA CATCGCGGCG GTTGTTTCCA TCGCCATTGC 451 CAAAACCAGC CGTTCGATTT CGGAACGTTC GGCGGCGGTA AATTGCGATT 501 CGTCGCCCAA CACTTCGGGC AGCCAGTCGA GCGGTGTCAA TTTGTCCGGC 551 CCGCTCAACA GCGCCGTCAT AAAACCTTGA ACCTCGTCGC AACGCATCGT 601 GTTGCCTTGT TCGCTTTTGG CATCCAACAA TTCGCTCAAC CGCCGTTTGG 651 ATGCTTCGGT AAATTTTCGG GAATCCATCA TTTTCCTTTT CAAATGGGTT 701 TTGCGCCCTA TTATCGCCGC AATGCCGTCT GA This corresponds to the amino acid sequence <SEQ ID 526; ORF 136>: ml36.pep 1 METNASILTA TRLVFSAAAA RTGIVPACFF AFPADGLRFV DDCLPVAVDI 51 RQCIRQLGFQ FRQLAFCELQ TDSAVFLF NTAQCHDGIK QLFKRFIIDG 101 FKPIGRHNIQ TVKISIAPCV KIAAAVFVFI QPQIGQFFIR HRGGCFHRHC 151 QNQPFDFGTF GGGKLRFVAQ HFGQPVERCQ FVRPAQQRRH KTLNLVATHR 201 VALFAFGIQQ FAQPPFGCFG KFSGIHHFPF QMGFAPYYRR NAV* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 136 shows 85.6% identity over a 209 aa overlap with a predicted ORF (ORF 136.ng) from N gonorrhoeae: ml36/gl36 10 20 30 40 ml36.pep METΝASILTATRLVFSAAAARTGIVPACFFAFPADGLRFVDDCLPV hMMMMIMM ! 11 ! 1111111 II MMIIMIM III gl36 MEIRFQTAFLRLVQMKTΝASILTATRLVFPAAAARTGIVPAGFFPFPADGLRFVDDRLPV 10 20 30 40 50 60 50 60 70 80 90 100 ml36.pep AVDIRQCIRQLGFQFRQLAFCELQTDSAVFLFWΝTAQCHDGIKQLFKRFIIDGFKPIGR llh I = 11 : 1 MMIII llhhlMMMhhll hlMMIIM gl36 AVDVCQRVRQFGRKFRQLAFGELQADΝAVFLFWΝAAHCHHGVKQLFKRFIIGGFKPIGR 70 80 90 100 110 120 110 120 130 140 150 160 ml36.pep HΝIQTVKISIAPCVKIAAAVFVFIQPQIGQFFIRHRGGCFHRHCQΝQPFDFGTFGGGKLR ' |: I ::j lllllh I = = I II I h I II 11 II II II II II 111 II II I II II 11 gi36 HΝVQTVKIGVAPSVKIAAALAVWEPQIGQLFIRHRGGCFHRHCQΝQPFDFGTFGGGKLR 130 140 150 160 170 180 170 180 190 200 210 220 mi36. pep FVAQHFGQPVERCQFVRPAQQRRHKTLΝLVATHRVALFAFGIQQFAQPPFGCFGKFSGIH M II MMI M M I I M M M M M M M MM M M MM M g i36 FVAQHFGQPVERCQFVRPAQQRRHKTLΝLVATHRVALFAFGIQX 190 200 210 220 230 240 ml36. pep HFPFQMGFAPYYRRΝAVX The following partial DΝA sequence was identified in N meningitidis <SEQ ID 527>: al36. seq 1 ATGGAAACAA ACGCTTCAAT TCTTACCGCA ACACGCCTTG TATTTTCTGC 51 CGCTGCCGCA CGGACAGGGA TCGTTCCTGC CTGTTTTTTC GCCTTCCCTG 101 CGGACGGTTT GCGGCTTGTT GATGACCGCC TGCCAGTAGC GGTAGATATC 151 CGCCAATGCA TAAGGCAACT CGGATTCCAG TTCCGCCAGC TCGCCTTCTG 201 TGAATTGCAG ACGGATAGTG CCGTTGTCCT CTTCGTCGTA AATACCGCCC 251 AATGCCATGA TGGGATAAAA CAACTCTTCA AACGCTTCAT CATCGACGGC 301 TTCAAACCAA TCGGTCGGCA CAATATCCAA ACCGTAAAGA TAAGCATTGC 351 ACCATGTGTA AAAATCGCTG CCGCCGTCTT CGTTTTCATA CAGCCACAAA 401 TCGGGCAGTT TTTTATCCGA CATCGCGGCG GTTGTTTCCA TCGCCATTGC 451 CAAAACCAGC CGTTCGATTT CGGAACGTTC GGCGGCGGTA AATTGCGATT 501 CGTCGCCCAA CACTTCGGGC AGCCAGTCGA GCGGTGTCAA TTTGTCCGGC 551 CCGCTCAACA GCGCCGTCAT AAAACCTTGA ACCTCGTCGC AACGCATCGT 601 GTTGCCTTGT TCGCTTTTGG CATCCAACAA TTCGCTCAAC CGCCGTTTGG 651 ATGCTTCGGT AAATTTTCGG GAATCCATCA TTTTCCTTTT CCAATGGGTT 701 TTGCGCCCTA TTATAGTGGA TTAAATTTAA ATCAGGACAA GGCGACGAAG 751 CCGCAGACAG TACAAATAGT ACGGCAAGGC GAGGCAACGC CGTACTGGTT 801 TAAATTTAAT CCACTATATC GCCGCAATGC CGTCTGA This corresponds to the amino acid sequence <SEQ ID 528; ORF 136. a>: al36.pep 1 METNASILTA TRLVFSAAAA RTGIVPACFF AFPADGLRLV DDRLPVAVDI 51 RQCIRQLGFQ FRQLAFCELQ TDSAWLFW NTAQCHDGIK QLFKRFIIDG 101 FKPIGRHNIQ TVKISIAPCV KIAAAVFVFI QPQIGQFFIR HRGGCFHRHC 151 QNQPFDFGTF GGGKLRFVAQ HFGQPVERCQ FVRPAQQRRH KTLNLVATHR 201 VALFAFGIQQ FAQPPFGCFG KFSGIHHFPF PMGFAPYYSG LNLNQDKATK 251 PQTVQIVRQG EATPYWFKFN PLYRRNAV* ml 36/al 36 98.3% identity in 238 aa overlap 10 20 30 40 50 60 ml36.pep METNAΞILTATRLVFSAAAARTGIVPACFFAFPADGLRFVDDCLPVAVDIRQCIRQLGFQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I al36 METNASILTATRLVFSAAAARTGIVPACFFAFPADGLRLVDDRLPVAVDIRQCIRQLGFQ 10 20 30 40 50 60 70 80 90 100 110 120 ml36 . pep FRQLAFCELQTDSAVFLFWNTAQCHDGIKQLFKRFIIDGFKPIGRHNIQTVKISIAPCV l l l l l l l l l l l l l l l I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al36 FRQLAFCELQTDSAWLFWNTAQCHDGIKQLFKRFIIDGFKPIGRHNIQTVKISIAPCV 70 80 90 100 110 120 130 140 150 160 170 180 ml36 . pep KIAAAVFVFIQPQIGQFFIRHRGGCFHRHCQNQPFDFGTFGGGKLRFVAQHFGQPVERCQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al36 KIAAAVFVFIQPQIGQFFIRHRGGCFHRHCQNQPFDFGTFGGGKLRFVAQHFGQPVERCQ 130 140 150 160 170 180 190 200 210 220 230 240 ml36. pep FVRPAQQRRHKTLNLVATHRVALFAFGIQQFAQPPFGCFGKFSGIHHFPFQMGFAPYYRR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I lllllll al36 FVRPAQQRRHKTLNLVATHRVALFAFGIQQFAQPPFGCFGKFSGIHHFPFPMGFAPYYSG 190 200 210 220 230 240 ml36. pep NAVX al36 LNLNQDKATKPQTVQIVRQGEATPYWFKFNPLYRRNAVX 250 260 270 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 529>: gl37.seq 1 ATGATTATCC ATCACcaaTT CGATCCCGTC CTCATCAGTA TCGGCCCGCT 51 TGCCGTCCGC TGGTATGCCT TAAGCTACAT CCTCGGATTT ATTCTTTTTA 101 CCTTTCTCGG CAGAAGGCGC ATCGCGCAAG GCTTGTCCGT TTTTACCAAA 151 GAATCGCTCG ACGACTTCCT GACATGGGGC ATTTTGGGCG TGATTTTGGG 201 CGGACGCTTG GGCTATGTCC TGTTTTACAA ATTCTCCGAC TACCTCGCCC 251 ATCCGCTTGA TATTTTCAAG GTATGGGAAG GCGGAATGTC GTTCCACGGC 301 GGCTTTTTGG GTGTAGTTAT TGCCATATGG TTGTTCAGCC GCAAGCACGG 351 CATCGGCTTC CTCAAACTGA TGGACACGGT CGCGCCGCTC GTTCCGCTGG 401 GTCTCGCTTC GGGACGTATC GGCAACTTTA TCAACGGCGA ACTTTGGGGA 451 CGCATTACCG ACATTAACGC ATTTTGGGCA ATGGGCTTCC CGCAAGCGCA 501 TTACGAAGAT GCCGAAGCCG CCGCGCACAA TCCGCTTTGG GCAGAATGGC 551 TGCAACAATA CGGTATGCTG CCGCGTCATC CCTCGCAGCT TTATCAGTTT 601 GCCCTTGAAG GCATCTGCCT GTTCGCCGTC GTTTGGCTGT TTTCCAAAAA 651 ACCGCGCCCG ACCGGGCAGA CTGCCGCGCT TTTTCTCGGC GGCTACGGCG 701 TGTTCCGCTT TATTGCCGAA TTTGCGCGCC AACCCGACGA CTATCTCGGG 751 CTGCTGACCT TGGGGCTGTC GATGGGGCAA TGGTTGAGCG TCCCGATGAT 801 TGTTTTGGGT ATCGTCGGCT TTGTCCGGTT CGGCATGAAA AAACAGCACT 851 GA This corresponds to the amino acid sequence <SEQ ID 530; ORF 137.ng>: gl37 . pep 1 MIIHHQFDPV LISIGPLAVR WYALSYILGF ILFTFLGRRR IAQGLSVFTK 51 ESLDDFLTWG ILGVILGGRL GYVLFYKFSD YLAHPLDIFK VWEGGMSFHG 101 GFLGWIAIW LFSRKHGIGF LKLMDTVAPL VPLGLASGRI GNFINGELWG 151 RITDINAFWA MGFPQAHYED AEAAAHNPLW AEWLQQYGML PRHPSQLYQF 201 ALEGICLFAV VWLFSKKPRP TGQTAALFLG GYGVFRFIAE FARQPDDYLG 251 LLTLGLSMGQ WLSVPMIVLG IVGFVRFGMK KQH* The following partial DNA sequence was identified in N meningitidis <SEQ ID 531>: ml37. seq 1 ATGATTACCC ATCCCCAATT CGATCCCGTC CTTATCAGTA TCGGCCCGCT 51 TGCCGTCCGC TGGTATGCCC TAAGCTACAT CCTCGGATTT ATTCTTTTTA 101 CCTTTCTCGG CAGAAGGCGC ATCGCGCAAG GCTTGTCCGT TTTTACCAAA 151 GAATCGCTCG ACGACTTCCT GACATGGGGC ATTTTGGGCG TAATTTTGGG 201 CGGGCGTTTG GGTTACGTCC TGTTTTACAA GTTTTCCGAC TACCTCGCCC 251 ATCCGCTTGA TATTTTCAAG GTATGGGAAG GCGGAATGTC GTTCCACGGC 301 GGCTTTTTGG GTGTAGTTAT TGCCATACGG TTGTTCGGCC GCAAACACGG 351 CATCGGCTTC CTCAAACTGA TGGATACGGT CGCACCGCTC GTTCCGCTGG 401 GTCTCGCTTC GGGACGTATC GGCAACTTCA TCAACGGCGA ACTTTGGGGA 451 CGCGTTACCG ACATCAACGC ATTTTGGGCA ATGGGCTTCC CGCAGGCGCG 501 TTACGAAGAT GCCGAAGCCG CCGCGCACAA TCCGCTTTGG GCAGAATGGC 551 TGCAACAATA CGGTATGCTG CCGCGTCATC CCTCGCAGCT TTATCAGTTT 601 GCACTTGAAG GCATCTGCCT GTTCACCGTC ATTTGGCTGT TCTCTAAAAA 651 ACAGCGGTCG ACCGGACAAG TCGCCTCGCT CTTCCTCGGC GGCTACGGCA 701 TATTCCGCTT CATTGCCGAA TTCGCACGCC AACCCGACGA CTATCTCGGG 751 CTGCTGACCT TGGGGCTGTC GATGGGGCAA TGGTTGAGCG TCCCGATGAT 801 TGTTTTGGGT ATCGTCGGCT TTGTCCGGTT CGGCATGAAA AAACAGCACT 851 GA This corresponds to the amino acid sequence <SEQ ID 532; ORF 137>: ml37.pep 1 MITHPQFDPV LISIGPLAVR WYALSYILGF ILFTFLGRRR IAQGLSVFTK 51 ESLDDFLTWG ILGVILGGRL GYVLFYKFSD YLAHPLDIFK VWEGGMSFHG 101 GFLGWIAIR LFGRKHGIGF LKLMDTVAPL VPLGLASGRI GΝFIΝGELWG 151 RVTDIΝAFWA MGFPQARYED AEAAAHNPLW AEWLQQYGML PRHPSQLYQF 201 ALEGICLFTV IWLFSKKQRS TGQVASLFLG GYGIFRFIAE FARQPDDYLG 251 LLTLGLSMGQ WLSVPMIVLG IVGFVRFGMK KQH* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 137 shows 95.4% identity over a 283 aa overlap with a predicted ORF (ORF 137.ng) from N gonorrhoeae: ml37/gl37 10 20 30 40 50 60 ml37. pep MITHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQGLSVFTKESLDDFLTWG II I MMMMMMMMMMMMMMMMMMMMMMMIMMMM gl37 MIIHHQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQGLSVFTKESLDDFLTWG 10 20 30 40 50 60 70 80 90 100 110 120 ml37.pep ILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGFLGWIAIRLFGRKHGIGF M M M M M M M M M I M M M M M M M M M I I M M M M M I h l M I M I gl37 ILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGFLGWIAIWLFSRKHGIGF 70 80 90 100 110 120 130 140 150 160 170 180 ml37. pep LKLMDTVAPLVPLGLASGRIGΝFIΝGELWGRVTDIΝAFWAMGFPQARYEDAEAAAHΝPLW gl37 LKLMDTVAPLVPLGLASGRIGΝFIΝGELWGRITDIΝAFWAMGFPQAHYEDAEAAAHΝPLW 130 140 150 160 170 180 190 200 210 220 230 240 ml37. pep AEWL QQ YGMLPRHPSQLYQFALEGICLFTVIWLFSKKQRSTGQVASLFLGGYGIFRFIAE gl37 AEWLQQYGMLPRHPSQLYQFALEGICLFAWWLFSKKPRPTGQTAALFLGGYGVFRFIAE 190 200 210 220 230 240 250 260 270 280 ml37. pep FARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVRFGMKKQHX M M M M M M M I M M M M M M M M I I M I M M M M gl37 FARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVRFGMKKQHX 250 260 270 280 The following partial DNA sequence was identified in N meningitidis <SEQ ID 533>: al37. seq 1 ATGATTACCC ATCCCCAATT CGACCCCGTC CTTATCAGTA TCGGCCCGCT 51 TGCCGTCCGC TGGTATGCCC TAAGCTACAT CCTCGGATTT ATTCTTTTTA 101 CCTTTCTCGG CAGAAGGCGC ATCGCGCAAG GCTTGTCCGT TTTTACCAAA 151 GAATCGCTCG ACGACTTCCT GACATGGGGC ATTTTGGGCG TAATTTTGGG 201 CGGGCGTTTG GGTTACGTCC TGTTTTACAA GTTTTCCGAC TACCTCGCCC 251 ATCCGCTTGA TATTTTCAAG GTATGGGAAG GCGGAATGTC GTTCCACGGC 301 GGCTTTTTGG GTGTAGTTAT TGCCATATGG TTGTTCGGTC GCAAACACGG 351 CATCGGCTTC CTCAAACTGA TGGACACGGT CGCACCGCTC GTTCCACTGG 401 GTCTCGCTTC GGGACGTATC GGCAACTTCA TCAACGGCGA ACTTTGGGGA 451 CGCGTTACCG ACATCAACGC ATTTTGGGCA ATGGGCTTCC CGCAGGCGCG 501 TTACGAAGAC CTCGAAGCCG CCGCGCACAA TCCGCTTTGG GCAGAATGGC 551 TGCAACAATA CGGTATGCTG CCGCGTCATC CCTCGCAGCT TTATCAGTTT 601 GCACTTGAAG GCATCTGCCT GTTCGCCGTC GTTTGGCTGT TCTCTAAAAA 651 ACAGCGGCCG ACCGGACAAG TCGCCTCACT CTTCCTCGGC GGCTACGGCA 701 TATTCCGCTT CATTGCCGAA TTTGCACGCC AACCCGACGA CTATCTCGGG 751 CTGCTGACCT TGGGGCTGTC GATGGGGCAA TGGTTGAGCG TCCCGATGAT 801 TGTTTTGGGT ATCGTCGGCT TTGTCCGGTT CGGCATGAAA AAACAGCACT 851 GA This corresponds to the amino acid sequence <SEQ ID 534; ORF 137.a>: al37.pep 1 MITHPQFDPV LISIGPLAVR WYALSYILGF ILFTFLGRRR IAQGLSVFTK 51 ESLDDFLTWG ILGVILGGRL GYVLFYKFSD YLAHPLDIFK VWEGGMSFHG 101 GFLGWIAIW LFGRKHGIGF LKLMDTVAPL VPLGLASGRI GΝFIΝGELWG 151 RVTDIΝAFWA MGFPQARYED LEAAAHΝPLW AEWLQQYGML PRHPSQLYQF 201 ALEGICLFAV VWLFSKKQRP TGQVASLFLG GYGIFRFIAE FARQPDDYLG 251 LLTLGLSMGQ WLSVPMIVLG IVGFVRFGMK KQH* ml37/al37 98.2% identity in 283 aa overlap 10 20 30 40 50 60 ml37.pep MITHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQGLSVFTKESLDDFLTWG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al37 MITHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQGLSVFTKESLDDFLTWG 10 20 30 40 50 60 70 80 90 100 110 120 ml37. pep ILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGFLGWIAIRLFGRKHGIGF I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I II al37 ILGVILGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGFLGVVIAIWLFGRKHGIGF 70 80 90 100 110 120 130 140 150 160 170 180 ml37. pep LKLMDTVAPLVPLGLASGRIGΝFIΝGELWGRVTDIΝAFWAMGFPQARYEDAEAAAHΝPLW I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I lllllllll al37 LKLMDTVAPLVPLGLASGRIGΝFIΝGELWGRVTDIΝAFWAMGFPQARYEDLEAAAHΝPLW 130 140 150 160 170 180 190 200 210 220 230 240 ml37. pep AEWLQQYGMLPRHPSQLYQFALEGICLFTVIWLFSKKQRSTGQVASLFLGGYGIFRFIAE I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I al37 AEWLQQYGMLPRHPSQLYQFALEGICLFAWWLFSKKQRPTGQVASLFLGGYGIFRFIAE 190 200 210 220 230 240 250 260 270 280 ml37.pep FARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVRFGMKKQHX I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I al37 FARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVRFGMKKQHX 250 260 270 280 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 535>: gl38.seq 1 ATGGAGTTTG AAAACATTAT TTCCGCCGCc gaCAAGGCGC GTATCCTTGC 51 CGAAGCACTG CCTTACAtcc gccgGTTTTC CGGTTCGGTC GCCGTCATCA 101 AGTATGGCGG CAACGCGATG ACCGAACCTG CCTTGAAAGA AGGGTTTGCC 151 CGCGATGTCG TGCTGCTGAA GCTGGTCGGC ATTCATCCCG TCATCGTTCA 201 CGGCGGCGGG CCGCAGATCA ATGCGATGCT TGAAAAAGTC GGCAAAAAGG 251 GCGAATTTGT CCAAGGAATG CGCGTTACCG ACAAAGAGAC GATGGATATT 301 GTCGAAATGG TATTGGGCGG GCACGTCAAC AAGGAAATCG TGTCGATGAT 351 TAACACATAT GGAGGGCACG CGGTCGGCGT GAGCGGGCGC GACGACCATT 401 TCATTAAGGC GAAGAAACTT TTGGTCGATA CGCCCGAACA GAATAGCGTG 451 GACATCGGAC AGGTCGGTAC GGTGGAAAGC ATCGATACCG GTTTGGTTAA 501 AGGGCTGATA GAACGCGGCT GCATTCCCGT CGTCGCCCCC GTCGGCGTAG 551 GTGAAAAAGG CGAAGCGTTC AACATCAACG CCGATTTGGT GGCAGGCAAA 601 TTGGCGGAAG AATTGAACGC CGAAAAACTC TTGATGATGA CGAAtatcgc 651 cgGTGTGATG GACAAAACGG GCAATCTGCT GACCAAACTC acgCCGAAAC 701 GGATTGATGG GCTGATTGCC GACGGCACGC TGTATGGCGG TATGCTGCCG 751 AAAATCGCTT CTGCGGTCGA AGCcgccgtc aACGGTGTGA AAGCCACGCA 801 CATCATCGAC GGCAGGTTGC CCAACGCGCT TTTGCTGGAA ATCTTTACCG 851 ATGCCGGTAT CGGGTCGATG ATTTTAGGCA GAGGGGAAGA TGCCTGA This corresponds to the amino acid sequence <SEQ ID 536; ORF 138.ng>: gl38.pep 1 MEFEΝIISAA DKARILAEAL PYIRRFSGSV AVIKYGGΝAM TEPALKEGFA 51 RDWLLKLVG IHPVIVHGGG PQIΝAMLEKV GKKGEFVQGM RVTDKETMDI 101 VEMVLGGHVΝ KEIVSMIΝTY GGHAVGVSGR DDHFIKAKKL LVDTPEQΝSV 151 DIGQVGTVES IDTGLVKGLI ERGCIPWAP VGVGEKGEAF ΝIΝADLVAGK 201 LAEELΝAEKL LMMTΝIAGVM DKTGΝLLTKL TPKRIDGLIA DGTLYGGMLP 251 KIASAVEAAV ΝGVKATHIID GRLPΝALLLE IFTDAGIGSM ILGRGEDA* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 537>: ml38.seq 1 ATGGAGTCTG AAAACATTAT TTCCGCCGCC GACAAGGCGC GTATCCTTGC 51 CGAAGCGCTG CCTTACATCC GCCGGTTTTC CGGTTCGGTC GCCGTCATCA 101 AATACGGCGG CAACGCGATG ACCGAACCTG CCTTGAAAGA AGGGTTTGCC 151 CGCGATGTCG TGCTGCTGAA GCTGGTCGGC ATTCATCCCG TCATCGTTCA 201 CGGCGGCGGG CCGCAGATCA ATGCGATGCT TGAAAAAGTC GGCAAAAAGG 251 GTGAGTTTGT CCAAGGAATG CGCGTTACCG ACAAAGAGGC GATGGATATT 301 GTCGAAATGG TGTTGGGCGG GCATGTCAAT AAAGAAATCG TGTCGATGAT 351 TAACACATAT GGCGGACACG CGGTCGGCGT AAGCGGACGC GACGACCATT 401 TCATTAAGGC GAAGAAACTT TTGATCGATA CGCCCGAACA GAATGGCGTG 451 GACATCGGAC AGGTCGGTAC GGTGGAAAGC ATCGATACCG GTTTGGTTAA 501 AGGGCTGATA GAACGTGGCT GCATTCCCGT CGTCGCCCCC GTCGGCGTAG 551 GTGAAAAAGG CGAAGCGTTC AACATCAACG CCGATTTGGT AGCAGGCAAA 601 TTGGCGGAAG AATTGAACGC CGAAAAACTC TTGATGATGA CGAATATCGC 651 CGGTGTGATG GACAAAACGG GCAATCTGCT GACCAAACTC ACGCCGAAAC 701 GGATTGATGA ACTGATTGCC GACGGCACGC TGTATGGCGG TATGCTGCCG 751 AAAATCGCTT CTGCGGTCGA AGCCGCCGTC AACGGTGTGA AAGCCACGCA 801 TATCATCGAC GGCAGGTTGC CCAACGCGCT TTTGCTGGAA ATCTTTACCG 851 ATGCCGGTAT CGGTTCGATG ATTTTGGGCG GTGGGGAAGA TGCCTGA This corresponds to the amino acid sequence <SEQ ID 538; ORF 138>: ml38.pep 1 MESEΝIISAA DKARILAEAL PYIRRFSGSV AVIKYGGΝAM TEPALKEGFA 51 RDWLLKLVG IHPVIVHGGG PQINAMLEKV GKKGEFVQGM RVTDKEAMDI 101 VEMVLGGHVN KEIVSMINTY GGHAVGVSGR DDHFIKAKKL LIDTPEQNGV 151 DIGQVGTVES IDTGLVKGLI ERGCIPWAP VGVGEKGEAF NINADLVAGK 201 LAEELNAEKL LMMTNIAGVM DKTGNLLTKL TPKRIDELIA DGTLYGGMLP 251 KIASAVEAAV NGVKATHIID GRLPNALLLE IFTDAGIGSM ILGGGEDA* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 138 shows 98.0% identity over a 298 aa overlap with a predicted ORF (ORF 138.ng) from N gonorrhoeae: ml38/gl38 10 20 30 40 50 60 ml38 -pep MESEΝIISAADKARILAEALPYIRRFSGSVAVIKYGGΝAMTEPALKEGFARDWLLKLVG II MIMMIMIMMMMMMMMMMMMMMMMMMIMMIMM gl38 MEFEΝIISAADKARILAEALPYIRRFSGSVAVIKYGGΝAMTEPALKEGFARDWLLKLVG 10 20 30 40 50 60 70 80 90 100 110 120 ml38.pep IHPVIVHGGGPQIΝAMLEKVGKKGEFVQGMRVTDKEAMDIVEMVLGGHVΝKEIVSMIΝTY gl38 IHPVIVHGGGPQIΝAMLEKVGKKGEFVQGMRVTDKETMDIVEMVLGGHVΝKEIVSMIΝTY 70 80 90 100 110 120 130 140 150 160 170 180 ml38. ep GGHAVGVSGRDDHFIKAKKLLIDTPEQΝGVDIGQVGTVESIDTGLVKGLIERGCIPWAP I II III I II MM MUM MM II II hi III II II Mill II II gl38 GGHAVGVSGRDDHFIKAKKLLVDTPEQΝSVDIGQVGTVESIDTGLVKGLIERGCIPWAP 130 140 150 160 170 180 190 200 210 220 230 240 ml38. pep VGVGEKGEAFΝIΝADLVAGKLAEELΝAEKLLMMTΝIAGVMDKTGΝLLTKLTPKRIDELIA MMMMMMMMMMMMMMMMMMMMMMMMMMMM III gl38 VGVGEKGEAFΝIΝADLVAGKLAEELΝAEKLLMMTΝIAGVMDKTGΝLLTKLTPKRIDGLIA 190 200 210 220 230 240 250 260 270 280 290 299 ml38.pep DGTLYGGMLPKIASAVEAAVΝGVKATHIIDGRLPΝALLLEIFTDAGIGSMILGGGEDAX MIMMMMMMMMMMMMMMMMMMMMMMMMM Mill g138 DGTLYGGMLPKIASAVEAAVΝGVKATHIIDGRLPΝALLLEIFTDAGIGSMI GRGEDAX 250 260 270 280 290 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 539>: al38. seq 1 ATGGAGTCTG AAAACATTAT TTCCGCCGCC GACAAGGCGC GTATCCTTGC 51 CGAAGCGCTG CCTTACATCC GCCGGTTTTC CGGTTCGGTC GCCGTCATCA 101 AATACGGCGG CAACGCGATG ACCGAACCTG CCTTGAAAGA AGGGTTTGCC 151 CGCGATGTCG TGCTGCTGAA GCTGGTCGGC ATTCATCCCG TCATCGTTCA 201 CGGCGGCGGG CCGCAGATCA ATGCGATGCT TGAAAAAGTC GGCAAAAAGG 251 GTGAGTTTGT CCAAGGAATG CGCGTTACCG ACAAAGAGGC GATGGATATT 301 GTCGAAATGG TGTTGGGCGG GCATGTCAAT AAAGAAATCG TGTCGATGAT 351 TAACACATAT GGCGGACACG CGGTCGGCGT AAGCGGACGC GACGACCATT 401 TCATTAAGGC GAAGAAACTT TTGATCGATA CGCCCGAACA GAATGGCGTG 451 GACATCGGAC AGGTCGGTAC GGTGGAAAGC ATCGATACCG GTTTGGTTAA 501 AGGGCTGATA GAACGTGGCT GCATTCCCGT CGTCGCCCCC GTCGGCGTAG 551 GTGAAAAAGG CGAAGCGTTC AACATCAACG CCGATTTGGT AGCAGGCAAA 601 TTGGCGGAAG AATTGAACGC CGAAAAACTC TTGATGATGA CGAATATCGC 651 CGGTGTGATG GACAAAACGG GCAATCTGCT GACCAAACTC ACGCCGAAAC 701 GGATTGATGA ACTGATTGCC GACGGCACGC TGTATGGCGG TATGCTGCCG 751 AAAATCGCTT CTGCGGTCGA AGCCGCCGTC AACGGCGTGA AAGCCACGCA 801 TATCATCGAC GGCAGGGTGC CCAACGCGCT TTTGCTGGAA ATCTTTACCG 851 ATGCCGGTAT CGGTTCGATG ATTTTGGGCG GTGGGGAAGA TGCCTGA This corresponds to the amino acid sequence <SEQ ID 540; ORF 138.a>: al38.pep 1 MESENIISAA DKARILAEAL PYIRRFSGSV AVIKYGGNAM TEPALKEGFA 51 RDWLLKLVG IHPVIVHGGG PQINAMLEKV GKKGEFVQGM RVTDKEAMDI 101 VEMVLGGHVN KEIVSMINTY GGHAVGVSGR DDHFIKAKKL LIDTPEQNGV 151 DIGQVGTVES IDTGLVKGLI ERGCIPWAP VGVGEKGEAF NINADLVAGK 201 LAEELNAEKL LMMTNIAGVM DKTGNLLTKL TPKRIDELIA DGTLYGGMLP 251 KIASAVEAAV NGVKATHIID GRVPNALLLE IFTDAGIGSM ILGGGEDA* ml38/al38 99.7% identity in 298 aa overlap 10 20 30 40 50 60 ml38. pep MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPALKEGFARDWLLKLVG I I I II I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al38 MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPALKEGFARDWLLKLVG 10 20 30 40 50 60 70 80 90 100 110 120 ml38. pep IHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVEMVLGGHVNKEIVSMINTY I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al38 IHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVEMVLGGHVNKEIVSMINTY 70 80 90 100 110 120 130 140 150 160 170 180 ml38.pep GGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVESIDTGLVKGLIERGCIPWAP I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al38 GGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVESIDTGLVKGLIERGCIPWAP 130 140 150 160 170 180 190 200 210 220 230 240 ml38.pep VGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAGVMDKTGNLLTKLTPKRIDELIA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al38 VGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAGVMDKTGNLLTKLTPKRIDELIA 190 200 210 220 230 240 250 260 270 280 290 299 ml38.pep DGTLYGGMLPKIASAVEAAVNGVKATHIIDGRLPNALLLEIFTDAGIGSMILGGGEDAX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I: I I I I I I I I I I I I I I I I I I I I I I I I I I al38 DGTLYGGMLPKIASAVEAAVNGVKATHIIDGRVPNALLLEIFTDAGIGSMILGGGEDAX 250 260 270 280 290 The following partial DNA sequence was identified in N.gonorrhoeae <SEQ ID 541>: gl39.seq 1 ATGCGAACCA CCTCAACCTT CCCTACAAAA ACTTTCAAAC CGGCTGCCAT 51 GGCGTTAGCT GTTGCAACAA CACTTTCTGC CTGCTTAggc ggcggcggag 101 gcGGCACTTC TGCTCCCGAC TTTAATGCAG GCGGCACCGG TATCGGCAGC 151 AACAGCAGGG CAACGATAGC GGAATCAGCA GCAGTATCTT ACGCCGGTAT 201 AAAAAACGAA ATGTGCAAAG ACAGAAGCAT GCTCTGTGCC GGTCGGGATG 251 ACGTTGCGGT TACAGACAGG GATGCCAAAA TCAAAGCCCC CCGAATCTGC 301 ATACCGGAGA CTTTTCAAAC CCAAATGACC AATATTAAGA ATATGATCAA 351 CCTCAAACCT GCAATTGAAG CAGGCTATAC AGGACGCGGG GTAGAGGTAG 401 GTATCGTCGA TACAGGCGAA TCCGTCGGCA GCATATCCTT TCCCGAACTG 451 TATGGCAGAA AAGAACACGG CTATAACGAA AATTACAAAA ACAAATTACA 501 AAAACTATAC GGCGTATATG CGGAAGGAAG CGCCTGA This corresponds to the amino acid sequence <SEQ ID 542; ORF 138.ng>: gl39.pep 1 MRTTSTFPTK TFKPAAMALA VATTLSACLG GGGGGTSAPD FNAGGTGIGS 51 NSRATIAESA AVSYAGIKNE MCKDRSMLCA GRDDVAVTDR DAKIKAPRIC 101 IPETFQTQMT NIKNMINLKP AIEAGYTGRG VEVGIVDTGE SVGSISFPEL 151 YGRKEHGYNE NYKNKLQKLY GVYAEGSA* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 543>: ml39.seq 1 ATGCGAACGA CCCCAACCTT CCCTACAAAA ACTTTCAAAC CGACTGCCAT 51 GGCGTTAGCT GTTGCAACAA CACTTTCTGC CTGCTTAGGC GGCGGCGGAG 101 GCGGCACTTC TGCGCCCGAC TTCAATGCAG GCGGTACCGG TATCGGCAGC 151 AACAGCAGAG CAACAACAGC GAAATCAGCA GCAGTATCTT ACGCCGGTAT 201 CAAGAACGAA ATGTGCAAAG ACAGAAGCAT GCTCTGTGCC GGTCGGGATG 251 ACGTTGCGGT TACAGACAGG GATGCCAAAA TCAATGCCCC CCCCCGAATC 301 TGCATACCGG AGACTTTCCA AACCCAAATG ACGCATtACA AGAATTTGAT 351 CAACCTCAAA CCTGCAATTG AAGCAGGCTA TACAGGACGC GGGGTAGAGG 401 TAGGTATCGT CGACACAGGC GAATCCGTCG GCAGCATATC CTTTCCCGAA 451 CTGTATGGCA GAAAAGAACA CGGCTATAAC GAAAATTACG AAAAACTATA 501 CGGCGTATAT GCGGAAGGAA GCGCCTGA This corresponds to the amino acid sequence <SEQ ID 544; ORF 138>: ml39.pep 1 MRTTPTFPTK TFKPTAMALA VATTLSACLG GGGGGTSAPD FNAGGTGIGS 51 NSRATTAKSA AVSYAGIKNE MCKDRSMLCA GRDDVAVTDR DAKINAPPRI 101 CIPETFQTQM THYKNLINLK PAIEAGYTGR GVEVGIVDTG ESVGSISFPE 151 LYGRKEHGYN ENYEKLYGVY AEGSA* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 138 shows 92.2% identity over a 179 aa overlap with a predicted ORF (ORF 138.ng) from N gonorrhoeae: ml39/gl39 10 20 30 40 50 60 ml39. pep MRTTPTFPTKTFKPTAMALAVATTLSACLGGGGGGTSAPDFΝAGGTGIGSΝSRATTAKSA MM MMMMhlMMMMMMMMMMMMMMMIMMMM hi gl39 MRTTSTFPTKTFKPAAMALAVATTLSACLGGGGGGTSAPDFΝAGGTGIGSΝSRATIAESA 10 20 30 40 50 60 70 80 90 100 110 120 ml39.pep AVSYAGIKΝEMCKDRSMLCAGRDDVAVTDRDAKIΝAPPRICIPETFQTQMTHYKΝLIΝLK M M M M M M M M M M M M M M M M I h l l 1 1 1 1 1 1 1 1 1 1 1 1 1 = I I M I M gl39 AVSYAGIKΝEMCKDRSMLCAGRDDVAVTDRDAKIKAP-RICIPETFQTQMTΝIKΝMIΝLK 70 80 90 100 110 130 140 150 160 170 ml39.pep PAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGYΝEΝY EKLYGVYAEGSAX MMMMMMMMMMMMMMMMMMIMMM MMMIMMM gl39 PAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGYΝEΝYKΝKLQKLYGVYAEGSAX 120 130 140 150 160 170 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 545>: al39. seq 1 ATGCGAACGA CCCCAACCTT CCCTACAAAA ACTTTCAAAC CGGCTGCCAT 51 GGCGTTAGCT GTTGCAACAA CACTTTCTGC CTGCTTAGGC GGCGGCGGAG 101 GCGGCACTTC TGCGCCCGAC TTCAATGCAG GCGGCACCGG TATCGGCAGC 151 AACAGCAGGG CAACAACAGC GAAATCAGCA GCAATATCTT ACGCCGGTAT 201 CAAGAACGAA ATGTGCAAAG ACAGAAGCAT GCTCTGTGCC GGTCGGGATG 251 ACGTTGCGGT TACAGACAGG GATGCCAAAA TCAATGCCCC CCCCCGAATC 301 TGCATACCGG AGACTTTACA AACCCAAATG ACGCAT.ACA AGAATTTGAT 351 CAACCTCAAA CCTGCAATTG AAGCAGGCTA TACAGGACGC GGGGTAGAGG 401 TAGGTATCGT CGACACAGGC GAATCCGTCG GCAGCATATC CTTTCCCGAA 451 CTGTATGGCA GAAAAGAACA CGGCTATAAC GAAAATTAC . AAAAACTATA 501 CGGCGTATAT GCGGAAGGAA GCGCCTGA This corresponds to the amino acid sequence <SEQ ID 546; ORF 139.a>: al39 -pep 1 MRTTPTFPTK TFKPAAMALA VATTLSACLG GGGGGTSAPD FNAGGTGIGS 51 NSRATTAKSA AISYAGIKNE MCKDRSMLCA GRDDVAVTDR DAKINAPPRI 101 CIPETLQTQM THXKNLINLK PAIEAGYTGR GVEVGIVDTG ESVGSISFPE 151 LYGRKEHGYN ENYXKLYGVY AEGSA* ml39/al39 97.1% identity in 175 aa overlap 10 20 30 40 50 60 ml39. pep MRTTPTFPTKTFKPTAMALAVATTLSACLGGGGGGTSAPDFNAGGTGIGSNSRATTAKSA I I I I I I I I I I I I II : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al39 MRTTPTFPTKTFKPAAMALAVATTLSACLGGGGGGTSAPDFNAGGTGIGSNSRATTAKSA 10 20 30 40 50 60 70 80 90 100 110 120 ml39. pep AVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPRICIPETFQTQMTHYKNLINLK I : I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I : I I I I I I lllllll al39 AISYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPRICIPETLQTQMTHXKNLINLK 70 80 90 100 110 120 130 140 150 160 170 ml39.pep PAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGYNENYEKLYGVYAEGSAX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I al39 PAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGYNENYXKLYGVYAEGSAX 130 140 150 160 170 The following partial DNA sequence was identified in N.gonorrhoeae <SEQ LD 547>: gl40. seq 1 Atgtcggcac gCGGCAAGGG GGCAGgctat ctcAACAGTA CCGGACGACa 51 TGTTCCCTTC CTGAGTGCCG CCAAAATCGG GCAGGATTAT TCTTTCTTCA 101 AAAATATCAA AACCGACGGC GGTCTGCTGG CTTCCCTCGA CAGCGTCGAA 151 AAAACAGCGG GCAGTGAAGG CGACACGCCG TCCTATTATG TCCGTCGCGG 201 CAATGCGGCA CGGACTGCTT CGGCAGCGGC ACATTCCGCG CCCGCCGGTC 251 TGAAACACGC CGTAGAACAG GGCGGCAGCA ATCTGGAAAA CCTGATGGTC 301 GAGCTGGATG CCTCCGAATC ATCCGCAACA CCCGAGACGG TTGAAACTGC 351 GGTCGCCGAC CGCACAGATA TGCCGGGCAT CCGCCTACGG CGCACAACTT 401 TCCGCACAGC GGCAGCCGTA CAGCATGCGA ATACCGCCGA CGGCGTACGc 451 aTCTTcaaCA GTCTCGCCGC TAccgTCTAt GccgACAGTG CCGCCGCCCA 501 TGccgATATG CAGGGACGCC GCCTGAAAGC CGTATCGGAC GGGTTGGACC 551 ACAACGGTAC GGGTCTGCGC GTCATCGCGC AAACCCAACA GGACGGTGGA 601 ACGTGGGAAC AGGGCGGTGT CGAAGGCAAA ATGCGCGGCA GTACCCAAAC 651 TATCGGCATT GCCGCGAAAA CCGGCGAAAA TACGACAGCA GCCGCCACAC 701 TGGGCATAGG ACGCAGCACA TGGAGCGAAA ACAGTGCAAA TGCAAAAACC 751 GACAGCATTA GTCTGTTTGC AGGCATACGG CACGATGTGG GCGATATCGG 801 CTATCTCAAA GGCCTGTTCT CctaCGGACG CTACAAAAAC AGCATCAGCC 851 GCAGCACCGG TGCGGATGAA TATGCGGAAG GCAGCGTCAA CGGCACGCTG 901 ATGCAGCTGG GCGCACTGGG TGGTGTCAAC GTTCCGTTTG CCGCAACGGG 951 AGATTTGACG GTTGAAGGCG GTCTGCGCCA CGACCTGCTC AAACAGGATG 1001 CATTCGCCGA AAAAGGCagt GCTTTGGGCT GGAGCGGCAA CAGCCTCACT 1051 GAAGGCACAC TGGTCGGACT CGCGGGTCTG AAACTGTCGC AACCCTTGAG 1101 CGATAAAGCC GTCCTGTCTG CGACGGCGGG CGTGGAACGC GACCTGAACG 1151 GACGCGACTA CGCGGTAACG GGCGGCTTTA CCGGCGCGGC TGCAGCAACC 1201 GGCAAGACGG GTGCACGCAA TATGCCGCAC ACCCGCCGGG TTGCCGGTCT 1251 GGGGGTGGAT GTCGAATTCG GCAACGGCTG GAACGGCTTG GCACGTTACA 1301 GCTACACCGG TTCCAAACAG TACGGCAACC ACAGCGGACA AATCGGCGTA 1351 GGCTACCGGT TCTGA This corresponds to the amino acid sequence <SEQ ID 548; ORF 140.ng>: gl40.pep 1 MSARGKGAGY LNSTGRHVPF LSAAKIGQDY SFFKNIKTDG GLLASLDSVE 51 KTAGSEGDTP SYYVRRGNAA RTASAAAHSA PAGLKHAVEQ GGSNLENLMV 101 ELDASESSAT PETVETAVAD RTDMPGIRLR RTTFRTAAAV QHANTADGVR 151 IFNSLAATVY ADSAAAHADM QGRRLKAVSD GLDHNGTGLR VIAQTQQDGG 201 TWEQGGVEGK MRGSTQTIGI AAKTGENTTA AATLGIGRST WSENSANAKT 251 DSISLFAGIR HDVGDIGYLK GLFSYGRYKN SISRSTGADE YAEGSVNGTL 301 MQLGALGGVN VPFAATGDLT VEGGLRHDLL KQDAFAEKGS ALGWSGNSLT 351 EGTLVGLAGL KLSQPLSDKA VLSATAGVER DLNGRDYAVT GGFTGAAAAT 401 GKTGARNMPH TRRVAGLGVD VEFGNGWNGL ARYSYTGSKQ YGNHSGQIGV 451 GYRF* The following partial DNA sequence was identified in N meningitidis <SEQ ID 549>: ml40. seq 1 ATGTCGGCAC GCGGCAAGGG GGCAGGCTAT CTCAACAGTA CCGGACGACG 51 TGTTCCCTTC CTGAGTGCCG CCAAAATCGG GCAGGATTAT TCTTTCTTCA 101 CAAACATCGA AACCGACGGC GGCCTGCTGG CTTCCCTCGA CAGCGTCGAA 151 AAAACAGCGG GCAGTGAAGG CGACACGCTG TCCTATTATG TCCGTCGCGG 201 CAATGCGGCA CGGACTGCTT CGGCAGCGGC ACATTCCGCG CCCGCCGGTC 251 TGAAACACGC CGTAGAACAG GGCGGCAGCA ATCTGGAAAA CCTGATGGTC 301 GAACTGGATG CCTCCGAATC ATCCGCAACA CCCGAGACGG TTGAAACTGC 351 GGCAGCCGAC CGCACAGATA TGCCGGGCAT CCGCCCCTAC GGCGCAACTT 401 TCCGCGCAGC GGCAGCCGTA CAGCATGCGA ATGCCGCCGA CGGTGTACGC 451 ATCTTCAACA GTCTCGCCGC TACCGTCTAT GCCGACAGTA CCGCCGCCCA 501 TGCCGATATG CAGGGACGCC GCCTGAAAGC CGTATCGGAC GGGTTGGACC 551 ACAACGGCAC GGGTCTGCGC GTCATCGCGC AAACCCAACA GGACGGTGGA 601 ACGTGGGAAC AGGGCGGTGT TGAAGGCAAA ATGCGCGGCA GTACCCAAAC 651 CGTCGGCATT GCCGCGAAAA CCGGCGAAAA TACGACAGCA GCCGCCACAC 701 TGGGCATGGG ACGCAGCACA TGGAGCGAAA ACAGTGCAAA TGCAAAAACC 751 GACAGCATTA GTCTGTTTGC AGGCATACGG CACGATGCGG GCGATATCGG 801 CTATCTCAAA GGCCTGTTCT CCTACGGACG CTACAAAAAC AGCATCAGCC 851 GCAGCACCGG TGCGGACGAA CATGCGGAAG GCAGCGTCAA CGGCACGCTG 901 ATGCAGCTGG GCGCACTGGG CGGTGTCAAC GTTCCGTTTG CCGCAACGGG 951 AGATTTGACG GTCGAAGGCG GTCTGCGCTA CGACCTGCTC AAACAGGATG 1001 CATTCGCCGA AAAAGGCAGT GCTTTGGGCT GGAGCGGCAA CAGCCTCACT 1051 GAAGGCACGC TGGTCGGACT CGCGGGTCTG AAGCTGTCGC AACCCTTGAG 1101 CGATAAAGCC GTCCTGTTTG CAACGGCGGG CGTGGAACGC GACCTGAACG 1151 GACGCGACTA CACGGTAACG GGCGGCTTTA CCGGCGCGAC TGCAGCAACC 1201 GGCAAGACGG GGGCACGCAA TATGCCGCAC ACCCGTCTGG TTGCCGGCCT 1251 GGGCGCGGAT GTCGAATTCG GCAACGGCTG GAACGGCTTG GCACGTTACA 1301 GCTACGCCGG TTCCAAACAG TACGGCAACC ACAGCGGACG AGTCGGCGTA 1351 GGCTACCGGT TCTGA This corresponds to the amino acid sequence <SEQ ID 550; ORF 140>: ml40.pep 1 MSARGKGAGY LΝSTGRRVPF LSAAKIGQDY SFFTΝIETDG GLLASLDSVE 51 KTAGSEGDTL SYYVRRGNAA RTASAAAHSA PAGLKHAVEQ GGSNLENLMV 101 ELDASESSAT PETVETAAAD RTDMPGIRPY GATFRAAAAV QHANAADGVR 151 IFNSLAATVY ADSTAAHADM QGRRLKAVSD GLDHNGTGLR VIAQTQQDGG 201 TWEQGGVEGK MRGSTQTVGI AAKTGENTTA AATLGMGRST WSENSANAKT 251 DSISLFAGIR HDAGDIGYLK GLFSYGRYKN SISRSTGADE HAEGSVNGTL 301 MQLGALGGVN VPFAATGDLT VEGGLRYDLL KQDAFAEKGS ALGWSGNSLT 351 EGTLVGLAGL KLSQPLSDKA VLFATAGVER DLNGRDYTVT GGFTGATAAT 401 GKTGARNMPH TRLVAGLGAD VEFGNGWNGL ARYSYAGSKQ YGNHSGRVGV 451 GYRF* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 140 shows 94.5% identity over a 454 aa overlap with a predicted ORF (ORF 140.ng) from N gonorrhoeae: ml40/gl40 10 20 30 40 50 60 ml40. pep MSARGKGAGYLΝSTGRRVPFLSAAKIGQDYSFFTΝIETDGGLLASLDSVEKTAGSEGDTL I I I I M I I I I I M I I h l l l l l l l l l l l l l l l l I h l l l l l l l l l l l l l l l l l l l l M gl40 MSARGKGAGYLΝSTGRHVPFLSAAKIGQDYSFFKΝIKTDGGLLASLDSVEKTAGSEGDTP 10 20 30 40 50 60 70 80 90 100 110 120 ml 0. pep SYYVRRGNAARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAAAD MMMMMMMMMMMMMMMMMMMMMMMMMIMMIhll gl40 SYYVRRGNAARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAVAD 70 80 90 100 110 120 130 140 150 160 170 180 ml40. pep RTDMPGIRPYGATFRAAAAVQHANAADGVRIFNSLAATVYADSTAAHADMQGRRLKAVSD gl40 RTDMPGIRLRRTTFRTAAAVQHANTADGVRIFNSLAATVYADSAAAHADMQGRRLKAVSD 130 140 150 160 170 180 190 200 210 220 230 240 ml40. pep GLDHNGTGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENTTAAATLGMGRST gl40 GLDHNGTGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTIGIAAKTGENTTAAATLGIGRST 190 200 210 220 230 240 250 260 270 280 290 300 ml40.pep WSENSANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRSTGADEHAEGSVNGTL gl40 WSENSANAKTDSISLFAGIRHDVGDIGYLKGLFSYGRYKNSISRSTGADEYAEGSVNGTL 250 260 270 280 290 300 310 320 330 340 350 360 ml40. pep MQLGALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGWSGNSLTEGTLVGLAGL gl40 MQLGALGGVNVPFAATGDLTVEGGLRHDLLKQDAFAEKGSALGWSGNSLTEGTLVGLAGL 310 320 330 340 350 360 370 380 390 400 410 420 ml40. pep KLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGKTGARNMPHTRLVAGLGAD 1 1 1 1 M 1 1 I I 1 1 l l l l h l g140 KLSQPLSDKAVLSATAGVERDLNGRDYAVTGGFTGAAAATGKTGARNMPHTRRVAGLGVD 370 380 390 400 410 420 430 440 450 ml40. pep VEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRFX I II I II II 1111111 = 1111111 II : 1111111 gl40 VEFGNGWNGLARYSYTGSKQYGNHSGQIGVGYRFX 430 440 450 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 551>: al40. seq 1 ATGTCGGCAG GCGGTAAGGG GGCAGGCTAT CTCAACCGTA CCGGACAACG 51 TGTTCCCTTC CTGAGTGCCG CCAAAATCGG GCGGGATTAT TCTTTCTTCA 101 CAAACATCGA AACCGACGGC GGTCTGCTGG CTTCCCTCGA CAGCGTCGAA 151 AAAACAGCGG GTAGTGAAGG CGACACGCTG TCCTATTATG TCCGTCGCGG 201 CAATGCGGCA CGGACTGCTT CGGCAGCGGC ACATTCCGCG CCCGCCGGTC 251 TGAAACACGC CGTAGAACAG GGCGGCAGCA ATCTGGAAAA CCTGATGGTC 301 GAACTGGATG CCTCCGAATC ATCCGCAACA CCCGAGACGG TTGAAACTGC 351 GGCCGCCGAC CGCACAGATA TGCCGGGCAT CCGCCCCTAC GGCGCAACTT 401 TCCGCGCAGC GGCAGCCGTA CAGCATGCGA ATGCCGCCGA CGGTGTACGC 451 ATCTTCAACA ATCTCGCCGC TACCGTCTAT GCCGACAGTA CCGCCGCCCA 501 TGCCGATATG CAGGGACGCC GCCTGAAAGC CGTATCGGAC GGGTTGGACC 551 ACAACGCTAC GGGTCTGCGC GTCATCGCGC AAACCCAACA GGACGGTGGA 601 ACGTGGGAAC AGGGCGGTGT TGAAGGCAAA ATGCGCGGCA GTACCCAAAC 651 CGTCGGCATT GCCGCGAAAA CCGGCGAAAA TACGACAGCA GCCGCCACAC 701 TGGGCATGGG ACACAGCACA TGGAGCGAAA ACAGTGCAAA TGCAAAAACC 751 GACAGCATTA GTCTGTTTGC AGGCATACGG CACGATGCGG GCGATATCGG 801 CTATCTCAAA GGCCTGTTCT CCTACGGACG CTACAAAAAC AGCATCAGCC 851 GCAGCACCGG TGCGGACGAA CATGCGGAAG GCAGCGTCAA CGGCACGCTG 901 ATGCAGCTGG GCGCACTGGG CGGTGTCAAC GTTCCGTTTG CCGCAACGGG 951 AGATTTGACG GTCGAAGGCG GTCTGCGCTA CGACCTGCTC AAACAGGATG 1001 CATTCGCCGA AAAAGGCAGT GCTTTGGGCT GGAGCGGCAA CAGCATCACT 1051 GAAGGCACAC TGGTCGGACT CGCGGGTCTG AAGCTGTCGC AACCCTTGAG 1101 CGATAAAGCC GTCCTGTTTG CAACGGCGGG CGTGGAACGC GACCTGAACG 1151 GACGCGACTA CACGGTAACG GGCGGCTTTA CCGGCGCGAC TGCAGCAACC 1201 GGCAAGACGG GGGCACGCAA TATGCCGCAC ACCCGCCTGG TTGCCGGTCT 1251 GGGCGCGGAT GTCGAATTCG GCAACGGCTG GAACGGCTTG GCACGTTACA 1301 GCTACGCCGG TTCCAAACAG TACGGCAACC ACAGCGGACG AGTCGGCGTA 1351 GGCTACCGGT TCTGA This corresponds to the amino acid sequence <SEQ ID 552; ORF 140.a>: al40.pep 1 MSAGGKGAGY LNRTGQRVPF LSAAKIGRDY SFFTNIETDG GLLASLDSVE 51 KTAGSEGDTL SYYVRRGNAA RTASAAAHSA PAGLKHAVEQ GGSNLENLMV 101 ELDASESSAT PETVETAAAD RTDMPGIRPY GATFRAAAAV QHANAADGVR 151 IFNNLAATVY ADSTAAHADM QGRRLKAVSD GLDHNATGLR VIAQTQQDGG 201 TWEQGGVEGK MRGSTQTVGI AAKTGENTTA AATLGMGHST WSENSANAKT 251 DSISLFAGIR HDAGDIGYLK GLFSYGRYKN SISRSTGADE HAEGSVNGTL 301 MQLGALGGVN VPFAATGDLT VEGGLRYDLL KQDAFAEKGS ALGWSGNSIT 351 EGTLVGLAGL KLSQPLSDKA VLFATAGVER DLNGRDYTVT GGFTGATAAT 401 GKTGARNMPH TRLVAGLGAD VEFGNGWNGL ARYSYAGSKQ YGNHSGRVGV 451 GYRF* ml40/al40 98.2% identity in 454 aa overlap 10 20 30 40 50 60 ml40. pep MSARGKGAGYLNSTGRRVPFLSAAKIGQDYSFFTNIETDGGLLASLDSVEKTAGSEGDTL III llllllll I I : I I I I I I I I I I I: I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al40 MSAGGKGAGYLNRTGQRVPFLSAAKIGRDYSFFTNIETDGGLLASLDSVEKTAGSEGDTL 10 20 30 40 50 60 70 80 90 100 110 120 ml 0. pep SYYVRRGNAARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAAAD I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I al40 SYYVRRGNAARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAAAD 70 80 90 100 110 120 130 140 150 160 170 180 ml40. pep RTDMPGIRPYGATFRAAAAVQHANAADGVRIFNSLAATVYADSTAAHADMQGRRLKAVSD I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I al40 RTDMPGIRPYGATFRAAAAVQHANAADGVRIFNNLAATVYADSTAAHADMQGRRLKAVSD 130 140 150 160 170 180 190 200 210 220 230 240 ml40. pep GLDHNGTGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENTTAAATLGMGRST I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I al40 GLDHNATGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENTTAAATLGMGHST 190 200 210 220 230 240 250 260 270 280 290 300 ml40.pep WSENSANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRSTGADEHAEGSVNGTL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I al40 WSENSANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRSTGADEHAEGSVNGTL 250 260 270 280 290 300 310 320 330 340 350 360 ml40. pep MQLGALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGWSGNSLTEGTLVGLAGL I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I II I I I I : I I I I I I I II I I al40 MQLGALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGWSGNSITEGTLVGLAGL 310 320 330 340 350 360 370 380 390 400 410 420 ml40. pep KLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGKTGARNMPHTRLVAGLGAD I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al40 KLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGKTGARNMPHTRLVAGLGAD 370 380 390 400 410 420 430 440 450 ml 0. pep VEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRFX I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al40 VEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRFX 430 440 450 The following partial DNA sequence was identified in N.gonorrhoeae <SEQ ID 553>: gl41. seq 1 atgagcttca aAAccgATGC CGAAACCGCC CAATCCTCCA CCATGCGCCC 51 GATTGGCGAA ATTGCCGCCA AGCTGGGTTT GAACGTTGAC AACATTGAGC 101 CTTACGGTCA TTACAAAGCC AAAATCAATC CTGCCGAAGC GTTCAAGCTG 151 CCGCAAAAAC AAGGCAGGCT GATTTTGGTT ACCGCCATCA ACCCGACTCC 201 GGCGGGCGAA GGCAAAACCA CCGTAACCAT CGGTTTGGCG GACGCATTGC 251 GCCATATCGG CAAAGACTCT GTGATTGCTT TGCGCGAGCC TTCTTTGGGT 301 CCGGTGTTCG GCGTGAAAGG CGGCGCGGCA GGCGGCGGCT ACGCGCAAGT 351 TTTGCCGATG GAAGACATCA ACCTGCACTT CACCGGCGAC TTCCACGCCA 401 TCGGTGCGGC GAATAACCTC CTCGCCGCCA TGCTCGACAA CCATATCTAC 451 CAAGGTAACG AGTTGAACAT CGACCCCAAA CGCGTGCTGT GGCGGCGCGT 501 GGTCGATATG AACGACCGCC AGTTGCGCAA CATCATCGAC GGTATGGGCA 551 AGCCTGTtga cggCGTGATG CGtcccGACG GCTTCGACAT CACCGTCGCC 601 TCCGAAGTGa tggcgGTATT CTGCCTTGCC AAAGACATCA GCGATTTGAA 651 AGAGCGTTtt gGCAATATTC TCGTCGCCTA CGCCAAAGAC GGCAGCCCCG 701 TTTACGCCAA AGATTTGAAG GCACACGGCG CGATGGCGGC ATTGCTAAAA 751 GATGCGATTA AGCCCAATTT GGTGCAAACC ATCGAAGGCA CTCCGGCCTT 801 TGTACACGGC GGCCCGTTCG CCAACATCGC CCACGGCTGC AACTCCGTTA 851 CCGCAACCCG TCTGGCGAAA CACCTTGCCG ATTACGCCGT AACCGAAGCA 901 GGCTTCGGCG CGGACTTGGG TGCGGAAAAA TTCTGCGACA TCAAATGCCG 951 CCTTGCCGGT TTGAAACCTG ATGCGGCAGT CGTCGTGGCG ACTGTCCGCG 1001 CCCTGAAATA CAACGGCGGC GTGGAACGCG CCAACCTTGG TGAAGAAAAC 1051 CTCGAAGCCT TGGCAAAAGG TTTGCCCAAC CTGTTGAAAC ACATTTCCAA 1101 CCTGAAAAAC GTATTCGGAC TGCCCGTCGT CGTTGCGCTC AACCGCTTCG 1151 TGTCCGACTC CGATGCCGAG TTGGCGATGA TTGAAAAAGC CTGTGCCGAA 1201 CACGGCGTTG AAGTTTCCCT GACCGAAGTG TGGGGCAAAG GCGGCGCGGG 1251 CGGCGCGGAT TTGGCGCGCA AAGTCGTCAA TGCCATCGAC AACCAACCTA 1301 ATAACTTCGG TTTCGCCTAC GATGTCGAGT TGGGCATCAA AGACAAAATC 1351 CGTGCGATTG CCCAAAAAGT GTACGGCGCG GAAGATGTCG ATTTCAGCGC 1401 GGAAGCGTCT GCCGAAATCG CCTCGCTGGA AAAACTGGGC TTGGACAAAA 1451 TGCCGATCTG CATGGCGAAA ACCCAATATT CATTGAGCGA CAACGCCAAA 1501 CTCTTGGGCT GCCCCGAAGG CTTCCGCATC GCCGTACGCG GTATCACTGT 1551 TTCCGCCGGC GCGGGCTTCA TCGTTGCGTT GTGCGGCAAT ATGATGAAAA 1601 TGCCGGGCCT GCCGAAAGTT CCGGCTGCCG AGAAAATCGA TGTGGACGAA 1651 CACGGCGTGA TTCACGGCTT GTTCTGA This corresponds to the amino acid sequence <SEQ ID 554; ORF 141.ng>: gl41.pep 1 MSFKTDAETA QSSTMRPIGE IAAKLGLNVD NIEPYGHYKA KINPAEAFKL 51 PQKQGRLILV TAINPTPAGE GKTTVTIGLA DALRHIGKDS VIALREPSLG 101 PVFGVKGGAA GGGYAQVLPM EDINLHFTGD FHAIGAANNL LAAMLDNHIY 151 QGNELNIDPK RVLWRRWDM NDRQLRNIID GMGKPVDGVM RPDGFDITVA 201 SEVMAVFCLA KDISDLKERF GNILVAYAKD GSPVYAKDLK AHGAMAALLK 251 DAIKPNLVQT IEGTPAFVHG GPFANIAHGC NSVTATRLAK HLADYAVTEA 301 GFGADLGAEK FCDIKCRLAG LKPDAAVWA TVRALKYNGG VERANLGEEN 351 LEALAKGLPN LLKHISNLKN VFGLPVWAL NRFVSDSDAE LAMIEKACAE 401 HGVEVSLTEV WGKGGAGGAD LARKWNAID NQPNNFGFAY DVELGIKDKI 451 RAIAQKVYGA EDVDFSAEAS AEIASLEKLG LDKMPICMAK TQYSLSDNAK 501 LLGCPEGFRI AVRGITVSAG AGFIVALCGN MMKMPGLPKV PAAEKIDVDE 551 HGVIHGLF* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 555>: ml41.seq 1 ATGAGCTTCA AAACCGATGC CGAAATCGCC CAATCCTCCA CCATGCGCCC 51 GATTGGCGAA ATTGCCGCCA AGCTTGGTCT GAATGCCGAC AACATTGAGC 101 CTTACGGTCA TTACAAGGCG AAAATCAATC CTGCCGAAGC GTTCAAACTG 151 CCGCAAAAAC AGGGCAGGCT GATTTTGGTT ACCGCCATCA ACCCGACTCC 201 GGCGGGCGAA GGCAAAACCA CCGTAACCAT CGGTTTGGCG GACGCGTTGC 251 GCCACATCGG CAAAGATGCC GTGATTGCCC TGCGCGAACC TTCTCTGGGG 301 CCGGTGTTCG GCGTGAAAGG CGGCGCGGCA GGCGGCGGCT ATGCCCAAGT 351 TTTGCCGATG GAAGACATCA ACCTGCACTT CACCGGAGAT TTTCACGCCA 401 TCGGTGCGGC AAATAATCTG CTTGCCGCGA TGCTCGACAA CCATATCTAC 451 CAAGGCAACG AGTTGAACAT CGACCCCAAA CGCGTGCTGT GGCGGCGCGT 501 GGTCGATATG AACGACCGCC AGTTGCGCAA CATCATCGAC GGCATGGGTA 551 AACCCGTTGA CGGCGTGATG CGTCCTGACG GTTTCGATAT TACCGTTGCT 601 TCCGAAGTGA TGGCGGTATT CTGTCTTGCC AAAGACATCA GCGATTTGAA 651 AGAGCGTTTG GGCAACATCC TTGTCGCCTA CGCCAAAGAC GGCAGCCCCG 701 TTTACGCCAA AGATTTGAAA GCGAATGGCG CGATGGCGGC ATTGCTTAAA 751 GATGCGATTA AGCCCAACTT GGTGCAAACC ATCGAAGGCA CGCCCGCCTT 801 CGTACACGGC GGCCCGTTCG CCAACATCGC CCACGGCTGC AACTCCGTAA 851 CCGCAACCCG TCTGGCGAAA CACCTTGCCG ATTACGCCGT AACCGAAGCA 901 GGCTTCGGCG CGGACTTGGG CGCGGAAAAA TTCTGCGACA TCAAATGCCG 951 CCTTGCCGGT TTGAAACCTG ATGCGGCTGT TGTCGTGGCG ACTGTCCGCG 1001 CGTTGAAATA TAACGGCGGC GTGGAACGCG CCAACCTCGG CGAAGAAAAT 1051 TTAGACGCTT TGGAAAAAGG TTTGCCCAAC CTGCTGAAAC ACATTTCCAA 1101 CCTGAAAAAC GTATTCGGAC TGCCCGTCGT CGTTGCGCTC AACCGCTTCG 1151 TGTCCGACGC CGATGCCGAG TTGGCGATGA TTGAAAAAGC CTGTGCCGAA 1201 CACGGCGTTG AAGTTTCCCT GACCGAAGTG TGGGGCAAAG GTGGTGCGGG 1251 CGGCGCGGAT TTGGCGCGCA AAGTCGTCAA CGCCATTGAA AGTCAAACCA 1301 ATAACTTCGG TTTCGCCTAC GATGTCGAGT TGGGCATCAA AGACAAAATC 1351 CGTGCGATTG CCCAAAAAGT GTACGGCGCG GAAGATGTTG ATTTCAGCGC 1401 GGAAGCGTCT GCCGAAATCG CTTCACTGGA AAAACTGGGC TTGGACAAAA 1451 TGCCGATCTG CATGGCGAAA ACCCAATACT CTTTGAGCGA CAACGCCAAA 1501 CTGTTGGGCT GCCCCGAAGA CTTCCGCATC GCCGTGCGCG GCATCACCGT 1551 TTCCGCAGGC GCAGGTTTCA TCGTCGCCCT GTGCGGCAAC ATGATGAAAA 1601 TGCCCGGCCT GCCCAAAGTT CCGGCTGCCG AGAAAATCGA TGTGGACGCA 1651 GAAGGCGTGA TTCACGGCTT GTTCTGA This corresponds to the amino acid sequence <SEQ ID 556; ORF 141>: ml41.pep 1 MSFKTDAEIA QSSTMRPIGE IAAKLGLNAD NIEPYGHYKA KINPAEAFKL 51 PQKQGRLILV TAINPTPAGE GKTTVTIGLA DALRHIGKDA VIALREPSLG 101 PVFGVKGGAA GGGYAQVLPM EDINLHFTGD FHAIGAANNL LAAMLDNHIY 151 QGNELNIDPK RVLWRRWDM NDRQLRNIID GMGKPVDGVM RPDGFDITVA 201 SEVMAVFCLA KDISDLKERL GNILVAYAKD GSPVYAKDLK ANGAMAALLK 251 DAIKPNLVQT IEGTPAFVHG GPFANIAHGC NSVTATRLAK HLADYAVTEA 301 GFGADLGAEK FCDIKCRLAG LKPDAAVWA TVRALKYNGG VERANLGEEN 351 LDALEKGLPN LLKHISNLKN VFGLPVWAL NRFVSDADAE LAMIEKACAE 401 HGVEVSLTEV WGKGGAGGAD LARKWNAIE SQTNNFGFAY DVELGIKDKI 451 RAIAQKVYGA EDVDFSAEAS AEIASLEKLG LDKMPICMAK TQYSLSDNAK 501 LLGCPEDFRI AVRGITVSAG AGFIVALCGN MMKMPGLPKV PAAEKIDVDA 551 EGVIHGLF* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 141 shows 97.5% identity over a 558 aa overlap with a predicted ORF (ORF 141. ng) from N gonorrhoeae: ml41/gl41 10 20 30 40 50 60 ml41.pep MSFKTDAEIAQSSTMRPIGEIAAKLGLΝADΝIEPYGHYKAKIΝPAEAFKLPQKQGRLILV l l l l l l l l I I M I I I I I I M I I I I I I h l M I I I M I I I I M I I I I I I I I I I I I I I M I gl41 MSFKTDAETAQSSTMRPIGEIAAKLGLΝVDΝIEPYGHYKAKIΝPAEAFKLPQKQGRLILV 10 20 30 40 50 60 70 80 90 100 110 120 ml41. pep TAINPTPAGEGKTTVTIGLADALRHIGKDAVI LREPSLGPVFGVKGGAAGGGYAQVLPM gl41 TAINPTPAGEGKTTVTIGLADALRHIGKDSVIALREPSLGPVFGVKGGAAGGGYAQVLPM 70 80 90 100 110 120 130 140 150 160 170 180 ml41. pep EDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPKRVLWRRWDMNDRQLRNIID MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM gl41 EDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPKRVLWRRWDMNDRQLRNIID 130 140 150 160 170 180 190 200 210 220 230 240 ml41. pep GMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKERLGNILVAYAKDGSPVYAKDLK gl41 GMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKERFGNILVAYAKDGSPVYAKDLK 190 200 210 220 230 240 250 260 270 280 290 300 ml41. pep ANGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANIAHGCNSVTATRLAKHLADYAVTEA hMMMMMMMMMMMMMMMMMMMMMMMMMMMMM gl41 AHGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANIAHGCNSVTATRLAKHLADYAVTEA 250 260 270 280 290 300 310 320 330 340 350 360 ml41.pep GFGADLGAEKFCDIKCRLAGLKPDAAVWATVRALKYNGGVERANLGEENLDALEKGLPN MMMMMMMMMMMMMMMMMMMMMIMMMIhll Mill gl41 GFGADLGAEKFCDIKCRLAGLKPDAAVWATVRALKYNGGVERANLGEENLEALAKGLPN 310 320 330 340 350 360 370 380 390 400 410 420 ml41.pep LLKHISNLKNVFGLPVWALNRFVSDADAELAMIEKACAEHGVEVSLTEVWGKGGAGGAD gl41 LLKHISNLKNVFGLPVWALNRFVSDSDAELAMIEKACAEHGVEVSLTEVWGKGGAGGAD 370 380 390 400 410 420 430 440 450 460 470 480 ml41.pep LARKWNAIESQTNNFGFAYDVELGIKDKIRAIAQKVYGAEDVDFSAEASAEIASLEKLG M 1111111 :: I MMMMMIMMMMMMMMMMMMMMMMMM gl41 LARKVVNAIDNQPNNFGFAYDVELGIKDKIRAIAQKVYGAEDVDFSAEASAEIASLEKLG 430 440 450 460 470 480 490 500 510 520 530 540 ml41.pep LDKMPICMAKTQYSLSDNAKLLGCPEDFRIAVRGITVSAGAGFIVALCGNMMKMPGLPKV MMMMMMMMMMMMM 111 II I II II II II II II I II II II II II II II gl41 LDKMPICMAKTQYSLSDNAKLLGCPEGFRIAVRGITVSAGAGFIVALCGNMMKMPGLPKV 490 500 510 520 530 540 550 559 ml41.pep PAAEKIDVDAEGVIHGLFX .-l1l1l1l1l1l1l1l1 gl4l PAAEKIDVDEHGVIHGLFX 550 The following partial DNA sequence was identified in N meningitidis <SEQ ID 557>: al41. seq 1 ATGAGTTTCA AAACCGATGC CGAAATCGCC CAATCCTCCA CCATGCGCCC 51 GATTGGCGAA ATTGCCGCCA AGCTGGGTTT GAACGTTGAC AACATTGAGC 101 CTTACGGTCA TTACAAAGCC AAAATCAATC CTGCCGAAGC GTTCAAACTG 151 CCGCAAAAAC AGGGCAGGCT GATTTTGGTT ACCGCCATCA ACCCGACTCC 201 GGCGGGCGAA GGTAAAACCA CCGTAACCAT CGGTTTGGCG GACGCATTGC 251 GCCATATCGG CAAAGACTCT GTGATTGCTT TGCGCGAGCC TTCTTTGGGT 301 CCGGTGTTCG GCGTGAAAGG CGGCGCGGCA GGCGGCGGCT ATGCCCAAGT 351 TTTGCCGATG GAAGACATCA ACCTGCACTT CACCGGAGAT TTTCACGCCA 401 TCGGTGCGGC AAATAATCTG CTTGCCGCGA TGCTCGACAA CCATATCTAC 451 CAAGGCAACG AGTTGAACAT CGACCCCAAA CGCGTGCTGT GGCGGCGCGT 501 GGTCGATATG AACGACCGCC AGTTGCGCAA CATCATCGAC GGCATGGGCA 551 AGCCTGTTGA CGGCGTGATG CGTCCTGACG GTTTCGATAT TACCGTTGCT 601 TCCGAAGTGA TGGCGGTATT CTGTCTTGCC AAAGACATCA GCGATTTGAA 651 AGAGCGTTTG GGCAACATCC TTGTCGCCTA CGCCAAAGAC GGCAGCCCCG 701 TTTACGCCAA AGATTTGAAA GCGAATGGCG CGATGGCGGC ATTGCTTAAA 751 GATGCGATTA AGCCCAACTT GGTGCAAACC ATCGAAGGCA CGCCCGCCTT 801 CGTACACGGC GGCCCGTTCG CCAACATCGC CCACGGCTGC AACTCCGTAA 851 CCGCAACCCG TCTGGCGAAA CACCTTGCCG ATTACGCCGT AACCGAAGCA 901 GGCTTCGGCG CGGACTTGGG CGCGGAAAAA TTCTGCGACA TCAAATGCCG 951 CCTTGCCGGT TTGAAACCTG ATGCGGCTGT TGTCGTGGCG ACTGTCCGCG 1001 CGTTGAAATA TAACGGCGGC GTGGAACGCG CCAACCTCGG CGAAGAAAAT 1051 TTAGACGCTT TGGAAAAAGG TTTGCCCAAC CTGCTGAAAC ACATTTCCAA 1101 CCTGAAAAAC GTATTCGGAC TGCCCGTCGT CGTTGCGCTC AACCGCTTCG 1151 TGTCCGACTC CGATGCCGAG TTGGCGATGA TTGAAAAAGC CTGTGCCGAA 1201 CACGGCGTTG AAGTTTCCCT GACCGAAGTG TGGGGCAAAG GTGGTGCGGG 1251 CGGCGCGGAT TTGGCGCGCA AAGTCGTCAA CGCCATTGAA AGTCAAACCA 1301 ATAACTTCGG TTTCGCCTAC GATGTCGAGT TGGGCATCAA AGACAAAATC 1351 CGTGCGATTG CCCAAAAAGT GTACGGCGCG GAAGATGTTG ATTTCAGCGC 1401 GGAAGCGTCT GCCGAAATCG CTTCACTGGA AAAACTGGGC TTGGACAAAA 1451 TGCCGATCTG CATGGCGAAA ACCCAATACT CTTTGAGCGA CAACGCCAAA 1501 CTGTTGGGCT GCCCCGAAGA CTTCCGCATC GCCGTGCGCG GCATCACCGT 1551 TTCCGCAGGC GCAGGTTTCA TCGTCGCCCT GTGCGGCAAC ATGATGAAAA 1601 TGCCCGGCCT GCCCAAAGTT CCGGCTGCCG AGAAAATCGA TGTGGACGCA 1651 GAAGGCGTGA TTCACGGCTT GTTCTGA This corresponds to the amino acid sequence <SEQ ID 558; ORF 141.a>: al41.pep 1 MSFKTDAEIA QSSTMRPIGE IAAKLGLNVD NIEPYGHYKA KINPAEAFKL 51 PQKQGRLILV TAINPTPAGE GKTTVTIGLA DALRHIGKDS VIALREPSLG 101 PVFGVKGGAA GGGYAQVLPM EDINLHFTGD FHAIGAANNL LAAMLDNHIY 151 QGNELNIDPK RVLWRRWDM NDRQLRNIID GMGKPVDGVM RPDGFDITVA 201 SEVMAVFCLA KDISDLKERL GNILVAYAKD GSPVYAKDLK ANGAMAALLK 251 DAIKPNLVQT IEGTPAFVHG GPFANIAHGC NSVTATRLAK HLADYAVTEA 301 GFGADLGAEK FCDIKCRLAG LKPDAAVWA TVRALKYNGG VERANLGEEN 351 LDALEKGLPN LLKHISNLKN VFGLPVWAL NRFVSDSDAE LAMIEKACAE 401 HGVEVSLTEV WGKGGAGGAD LARKWNAIE SQTNNFGFAY DVELGIKDKI 451 RAIAQKVYGA EDVDFSAEAS AEIASLEKLG LDKMPICMAK TQYSLSDNAK 501 LLGCPEDFRI AVRGITVSAG AGFIVALCGN MMKMPGLPKV PAAEKIDVDA 551 EGVIHGLF* ml41/al41 99.5% identity in 558 aa overlap 10 20 30 40 50 60 ml41.pep MSFKTDAEIAQSSTMRPIGEIAAKLGLNADNIEPYGHYKAKINPAEAFKLPQKQGRLILV I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al41 MSFKTDAEIAQSSTMRPIGEIAAKLGLNVDNIEPYGHYKAKINPAEAFKLPQKQGRLILV 10 20 30 40 50 60 70 80 90 100 110 120 ml41. pep TAINPTPAGEGKTTVTIGLADALRHIGKDAVIALREPSLGPVFGVKGGAAGGGYAQVLPM I I II I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al41 TAINPTPAGEGKTTVTIGLADALRHIGKDSVIALREPSLGPVFGVKGGAAGGGYAQVLPM 70 80 90 100 110 120 130 140 150 160 170 180 ml41.pep EDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPKRVLWRRWDMNDRQLRNIID I I I I I I I I I I I I I I I I I I I I I I I I I II I II I I I I I I I I I I I I I I I I I I I I I I I I I I II I I al41 EDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPKRVLWRRWDMNDRQLRNIID 130 140 150 160 170 180 190 200 210 220 230 240 ml41.pep GMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKERLGNILVAYAKDGSPVYAKDLK I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al 1 GMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKERLGNILVAYAKDGSPVYAKDLK 190 200 210 220 230 240 250 260 270 280 290 300 ml41. pep ANGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANIAHGCNSVTATRLAKHLADYAVTEA I I I I I I I I II I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al41 ANGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANIAHGCNSVTATRLAKHLADYAVTEA 250 260 270 280 290 300 310 320 330 340 350 360 ml41. pep GFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRALKYNGGVERANLGEENLDALEKGLPN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I al41 GFGADLGAEKFCDIKCRLAGLKPDAAVWATVRALKYNGGVERANLGEENLDALEKGLPN 310 320 330 340 350 360 370 380 390 400 410 420 ml41. pep LLKHISNLKNVFGLPVWALNRFVSDADAELAMIEKACAEHGVEVSLTEVWGKGGAGGAD I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al41 LLKHISNLKNVFGLPVWALNRFVSDSDAELAMIEKACAEHGVEVSLTEVWGKGGAGGAD 370 380 390 400 410 420 430 440 450 460 470 480 ml41. pep LARKWNAIESQTNNFGFAYDVELGIKDKIRAIAQKVYGAEDVDFSAEASAEIASLEKLG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I al41 LARKVVNAIESQTNNFGFAYDVELGIKDKIRAIAQKVYGAEDVDFSAEASAEIASLEKLG 430 440 450 460 470 480 490 500 510 520 530 540 ml41. pep LDKMPICMAKTQYSLSDNAKLLGCPEDFRIAVRGITVSAGAGFIVALCGNMMKMPGLPKV I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I al41 LDKMPICMAKTQYSLSDNAKLLGCPEDFRIAVRGITVSAGAGFIVALCGNMMKMPGLPKV 490 500 510 520 530 540 550 559 ml41.pep PAAEKIDVDAEGVIHGLFX I I I I I I I I I I I I I I I I I I I al 1 PAAEKIDVDAEGVIHGLFX 550 The following partial DNA sequence was identified in N.gonorrhoeae <SEQ ID 559>: gl42. seq 1 ATGCGTGCCG ATTTCATGTT TGCCGACAAT ATGCCCGTGC AGGTGCGCCA 51 ACGCGCCTTC TATTTCAAGT TGTCCCGTTT TGCCGCGATG CCAAATATGG 101 TAGGCAAACC GCTCTTCGGG CGACAGGCCG GTCAGCCCGG CAAAATGTTC 151 GGCAACATCC TGATGTTCGT CCGCCAGCAT ATTGATGCAG AGgCTGCCGT 201 TTTCCGACAG GATcggaATG AttcgCGCAC TCCGGTTTAT GCACAGCATC 251 ACGGTCGGCG GCTCGTCGGT AACCGGCGCA ACCGCCGTCA TTGTAATGCC 301 GTAACGCCCT GCCGCACCGT CTGTCGTGAT GACATGAACG CCTGCCGCAC 351 AGGATGCCAT CGCATCACGG AACGAAGTTT GAAAAGTTTT CTGCAAATCC 401 GCCATTTTTC CCCTTTAAAC CGTCCCCTAT ATAAGAATGC TGCACACAAG 451 GCATCCCCCC ATGTGCAGCA GTTCTGA This corresponds to the amino acid sequence <SEQ ID 560; ORF 142.ng>: gl42.pep 1 MRADFMFADN MPVQVRQRAF YFKLSRFAAM PNMVGKPLFG RQAGQPGKMF 51 GNILMFVRQH IDAEAAVFRQ DRNDSRTPVY AQHHGRRLVG NRRNRRHCNA 101 VTPCRTVCRD DMNACRTGCH RITERSLKSF LQIRHFSPLN RPLYKNAAHK 151 ASPHVQQF* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 561>: ml4 .seq 1 ATGCGTGCCG ATTTCATGTT TGCCGACAAT ATGCCCGTGC AGGTGCGCCA 51 ACGCGCCCTC TATTTCAAGT TGTCCCGTTT TGCCGCGATG CCAGATGTGG 101 TAGGCAAACC GCTCTTCGGG CGACAGGCCG GTCAGCCCGG CAAAATGTTC 151 GGCAACATCC TGATGTTCGT CCGCCAGCGT ATTGATGCAG AGGCTGCCGT 201 TTTCCGACAG GATCGGAATG ATTCGCGCAC TCCGGTTGAT GCACAGCATC 251 ACGGTCGGCG GCTCGTCGGT AACCGGCGCG ACCGCCGTCA TTGTAATGCC 301 GTAACGCCCT GCCGCACCGT CTGTCGTGAT GACATGAACG CCTGCCGCGC 351 AAGATGCCAT CGCATCACGG AACGAAGTTT GAAAATTTTT CTGCAAATCC 401 GCCATTTTTC CCCTTTAAAC TGTCCCCTAT ATAAGAATGC TGCACACAAG 451 GCATCCCCCC ATGTGCAGCA GTTTTGA This corresponds to the amino acid sequence <SEQ LD 562; ORF 142>: ml42.pep 1 MRADFMFADN MPVQVRQRAL YFKLSRFAAM PDWGKPLFG RQAGQPGKMF 51 GNILMFVRQR IDAEAAVFRQ DRNDSRTPVD AQHHGRRLVG NRRDRRHCNA 101 VTPCRTVCRD DMNACRARCH RITERSLKIF LQIRHFSPLN CPLYKNAAHK 151 ASPHVQQF* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 142 shows 93.7% identity over a 158 aa overlap with a predicted ORF (ORF 142.ng) from N gonorrhoeae: ml42/gl42 10 20 30 40 50 60 ml42.pep MRADFMFADΝMPVQVRQRALYFKLSRFAAMPDWGKPLFGRQAGQPGKMFGΝILMFVRQR g142 MRADFMFADΝMPVQVRQRAFYFKLSRFAAMPΝMVGKPLFGRQAGQPGKMFGΝILMFVRQH 10 20 30 40 50 60 70 80 90 100 110 120 ml42. pep IDAEAAVFRQDRΝDSRTPVDAQHHGRRLVGΝRRDRRHCΝAVTPCRTVCRDDMΝACRARCH M M M M I M M M M M I I I I I I I I I I I I h I I 1 1 I I I I I I I I I I I I I I I I I h I I gl42 IDAEAAVFRQDRΝDSRTPVYAQHHGRRLVGΝRRΝRRHCΝAVTPCRTVCRDDMΝACRTGCH 70 80 90 100 110 120 130 140 150 159 ml42.pep RITERSLKIFLQIRHFSPLΝCPLYKΝAAHKASPHVQQFX l l l l l l l l 1 1 1 1 1 1 I I 1 1 1 M M M M M M M M M gl42 RITERSLKSFLQIRHFSPLΝRPLYKΝAAHKASPHVQQFX 130 140 150 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 563>: al42.seq 1 ATGCGTGCCG ATTTCATGTT TGCCGACAAT ATGCCCGTGC AGGTGCGCCA 51 ACGCGCCCTC TATTTCAAGT TGTCCCGTTT TGCCGCGATG CCAGATGTGG 101 TAGGCAAACC GCTCTTCGGG CGACAGGCCG GTCAGCCCGG CAAAATGTTC 151 GGCAACATCC TGATGTTCGT CCGCCAGCGT ATTGATGCAG AGGCTGCCGT 201 TTTCCGACAG GATCGGAATG ATTCGCGCAC TCCGGTTGAT GCACAGCATC 251 ACGGTCGGCG GCTCGTCCGT AACCGGCGCA ACCGCCGTCA TTGTAATGCC 301 GTAACGCCCT GCCGCACCGT CTGTCGTGAT GACATGAACG CCTGCCGCAC 351 AGGATGCCAT CGCATCACGG AACGAAGTTT GAAAAGTTTT CTGCAAATCC 401 GCCATTTTTC CCCTTTAAAC TGTCCCCTAT ATAAGAATGC TGCACACAAG 451 GCACCCCCCA TGTGCAGCAG TTCTGATTCA AAAAGCCGTC GGTCGGACAT 501 TTCCGCGCGT TACGGCGTAT TACGAGTTCA ACGCATCCTC GATTTTGGCA 551 AGTTCTGCCA ACAGGTCTTT AAGCAGCAGC ATTTTCTCGC GGCCCAGCAC 601 TTCCTCGATA GCGTCGTAAC GCTCGTCCAC TTCTTCGCCG ATTTCCTCAT 651 ACAGCTTCTC GCCCTCGGCA GTCAGCTTCA GAAAAACACG TCGTTGGTCG 701 TTGGAAGGTT TCAGGCGGAC AACCAAACCC GCTTTTTCAA GGCGGGTCAG 751 GATACCGGTC AGGCTGGGGC GCAAAATGCA CGCCTGATTC GCCAAATCTT 801 GAAAGTCCAG CGTGCCGTTT TCCGCCAAAA GACGGATAAT CCGCCATTGC 851 TGATCGGTAA TATTCGCCTG ATTCAGAATA GGCCTGAATT GGGTCATCAG 901 GGCTTCCCTT GCCTGTATCA GACCGATATT GATAGACGCA TGTTTTGA This corresponds to the amino acid sequence <SEQ ID 564; ORF 142.a>: al42.pep 1 MRADFMFADN MPVQVRQRAL YFKLSRFAAM PDWGKPLFG RQAGQPGKMF 51 GNILMFVRQR IDAEAAVFRQ DRNDSRTPVD AQHHGRRLVR NRRNRRHCNA 101 VTPCRTVCRD DMNACRTGCH RITERSLKSF LQIRHFSPLN CPLYKNAAHK 151 APPMCSSSDS KSRRSDISAR YGVLRVQRIL DFGKFCQQVF KQQHFLAAQH 201 FLDSWTLVH FFADFLIQLL ALGSQLQKNT SLWGRFQAD NQTRFFKAGQ 251 DTGQAGAQNA RLIRQILKVQ RAVFRQKTDN PPLLIGNIRL IQNRPELGHQ 301 GFPCLYQTDI DRRMF* ml42/al42 96.1% identity in 153 aa overlap 10 20 30 40 50 60 ml42. pep MRADFMFADNMPVQVRQRALYFKLSRFAAMPDWGKPLFGRQAGQPGKMFGNILMFVRQR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al42 MRADFMFADNMPVQVRQRALYFKLSRFAAMPDWGKPLFGRQAGQPGKMFGNILMFVRQR 10 20 30 40 50 60 70 80 90 100 110 120 ml42. pep IDAEAAVFRQDRNDSRTPVDAQHHGRRLVGNRRDRRHCNAVTPCRTVCRDDMNACRARCH I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I : II al42 IDAEAAVFRQDRNDSRTPVDAQHHGRRLVRNRRNRRHCNAVTPCRTVCRDDMNACRTGCH 70 80 90 100 110 120 130 140 150 159 ml42.pep RITERSLKIFLQIRHFSPLNCPLYKNAAHKASPHVQQFX llllllll I I I I I I I I I I I I I I I I I I I I I I I al42 RITERSLKSFLQIRHFSPLNCPLYKNAAHKAPPMCSSSDSKSRRSDISARYGVLRVQRIL 130 140 150 160 170 180 al42 DFGKFCQQVFKQQHFLAAQHFLDSVVTLVHFFADFLIQLLALGSQLQKNTSLVVGRFQAD 190 200 210 220 230 240 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 565>: gl43. seq 1 ATGTTGAGCT TCGGCTATCT CGGCGTTCAG ACGGCCTTTA CCCTGCAAAG 51 CTCGCAGATG AGCCGCATTT TTCAAACGCT AGGCGCAGAC CCGCACAATT 101 TGGGCTGGTT TTTCATCCTG CCGCCGCTGG CGGGGATGCT GGTTCAGCCG 151 ATAGTGgGCT ACTACTCAGA CCGCACTTGG AAGCCGCGCT TGGGCGGCCG 201 CCGCCTGCCG TATCTGCTTT ACGGCACGCT GATTGCGGTC ATCGTGATGA 251 TTTTGATGCC GAACTCGGGC AGCTTCGGTT TCGGCTATGC GTCGCTGGCG 301 GCCTTGTCGT TCGGCGCGCT GATGATTGCG CTGTTGGACG TGTCGTCGAA 351 TATGGCGATG CAGCCGTTTA AGATGATGGT CGGCGATATG GTCAACGAGG 401 AGCAGAAAAG CTACGCCTAC GGGATTCAAA GTTTCTTAGC GAATACGGAC 451 GCGGTTGTGG CAGCGATTCT GCCGTTTGTG TTcgcgtata TCGGTTTGGC 501 GAACACTGCC GAGAAAGGCG TTGTGCCACA AACCGTGGTC GTAGCATTCT 551 ATGTGGGTGC GGCGTTACTG ATTATTACCA GTGCGTTCAC AATCTCCAAA 601 GTCAAAGAAT ACGACCCGGA AACCTACGCC CGTTACCACG GCATCGATGT 651 CGCCGCGAAT CAGGAAAAAG CCAACTGGTT CGAACTCTTA AAAACCGCGC 701 CTAAAGTGTT TTGGACGGTT ACTCCGGTAC AGTTTTTCTG CTGGTTCGCC 751 TTCCGGTATA TGTGGACTTA CTCGGCAGGC GCGATTGCAG AAAACGTCTG 801 GCACACTACC GATGCGTCTT CCGTAGGCCA TCAGGAGGCG GGCAACCGGT 851 ACGGCGTTTT GGCGGCGGTG TAGTCGGTTG CGGCGGTGAT TTGTTCGTTT 901 ATTCTGGCAA AAGTACCGAA TAAATACCAT AAGGCGGGTT ATTTCGGCTG 951 TTTGGCTTTG GGCGCGCTCG GTTTCTTCTC TATCTTCTTC ATCTACAATC 1001 AATACGCACT CATCCTGTCT TATATCTTAA TCGGCATCGC TTGGGCGGGC 1051 ATTATCACTT ATCCGCTGAC GATTGTGGCC AACGCTTTGT CGGGCAAACA 1101 CATGGATACT TATTTGGGCC TGTttaacgg ctctgtCTGT ATGCcgcaaa 1151 tcgTcgctTC GctgttgAGT TTCGTGCTTT TCCCGATGCT GGGCGGCCAT 1201 CAGGCAACCA TGTTCTTGGT TGCAGGCGCA GTCTTGCTGC TGGGAGCCTT 1251 CTCAGTCTGT CTGATTAAAG AGATCCACGG CGGGGTTTGA This corresponds to the amino acid sequence <SEQ ID 566; ORF 143.ng>: gl43.pep 1 MLSFGYLGVQ TAFTLQSSQM SRIFQTLGAD PHNLGWFFIL PPLAGMLVQP 51 IVGYYSDRTW KPRLGGRRLP YLLYGTLIAV IVMILMPNSG SFGFGYASLA 101 ALSFGALMIA LLDVSSNMAM QPFKMMVGDM VNEEQKSYAY GIQSFLANTD 151 AWAAILPFV FAYIGLANTA EKGWPQTVV VAFYVGAALL IITSAFTISK 201 VKEYDPETYA RYHGIDVAAN QEKANWFELL KTAPKVFWTV TPVQFFCWFA 251 FRYMWTYSAG AIAENVWHTT DASSVGHQEA GNRYGVLAAV *SVAAVICSF 301 ILAKVPNKYH KAGYFGCLAL GALGFFSIFF IYNQYALILS YILIGIAWAG 351 IITYPLTIVA NALSGKHMDT YLGLFNGSVC MPQIVASLLS FVLFPMLGGH 401 QATMFLVAGA VLLLGAFSVC LIKEIHGGV* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 567>: ml43. seq 1 ATGCTCAGTT TCGGCTTTCT CGGCGTTCAG ACGGCCTTTA CCCTGCAAAG 51 CTCGCAAATG AGCCGCATTT TTCAAACGCT AGGCGCAGAC CCGCACAATT 101 TGGGCTGGTT TTTCATCCTG CCGCCGCTGG CGGGGATGCT GGTGCAGCCG 151 ATTGTCGGCC ATTACTCCGA CCGCACTTGG AAGCCGCGTT TGGGCGGCCG 201 CCGTCTGCCG TATCTGCTTT ATGGCACGCT GATTGCGGTT ATTGTGATGA 251 TTTTGATGCC GAACTCGGGC AGCTTCGGTT TCGGCTATGC GTCGCTGGCG 301 GCTTTGTCGT TCGGCGCGCT GATGATTGCG CTGTTAGACG TGTCGTCAAA 351 TATGGCGATG CAGCCGTTTA AGATGATGGT CGGCGACATG GTCAACGAGG 401 AGCAGAAAGG CTACGCCTAC GGGATTCAAA GTTTCTTAGC AAATACGGGC 451 GCGGTCGTGG CGGCGATTCT GCCGTTTGTG TTTGCGTATA TCGGTTTGGC 501 GAACACCGCC GAGAAAGGCG TTGTGCCGCA GACCGTGGTC GTGGCGTTTT 551 ATGTGGGTGC GGCGTTGCTG GTGATTACCA GCGCGTTCAC GATTTTCAAA 601 GTGAAGGAAT ACGATCCGGA AACCTACGCC CGTTACCACG GCATCGATGT 651 CGCCGCGAAT CAGGAAAAAG CCAACTGGAT CGAACTCTTG AAAACCGCGC 701 CTAAGGCGTT TTGGACGGTT ACTTTGGTGC AATTCTTCTG CTGGTTCGCC 751 TTCCAATATA TGTGGACTTA CTCGGCAGGC GCGATTGCGG AAAACGTCTG 801 GCACACCACC GATGCGTCTT CCGTAGGTTA TCAGGAGGCG GGTAACTGGT 851 ACGGCGTTTT GGCGGCGGTG CAGTCGGTTG CGGCGGTGAT TTGTTCGTTT 901 GTATTGGCGA AAGTGCCGAA TAAATACCAT AAGGCGGGTT ATTTCGGCTG 951 TTTGGCTTTG GGCGCGCTCG GCTTTTTCTC CGTTTTCTTC ATCGGCAACC 1001 AATACGCGCT GGTGTTGTCT TATACCTTAA TCGGCATCGC TTGGGCGGGC 1051 ATTATCACTT ATCCGCTGAC GATTGTGACC AACGCCTTGT CGGGCAAGCA 1101 TATGGGCACT TACTTGGGCT TGTTTAACGG CTCTATCTGT ATGCCTCAAA 1151 TCGTCGCTTC GCTGTTGAGT TTCGTGCTTT TCCCTATGCT GGGCGGCTTG 1201 CAGGCCACTA TGTTCTTGGT AGGGGGCGTC GTCCTGCTGC TGGGCGCGTT 1251 TTCCGTGTTC CTGATTAAAG AAACACACGG CGGGGTTTGA This corresponds to the amino acid sequence <SEQ ID 568; ORF 143>: ml 3.pep 1 MLSFGFLGVQ TAFTLQSSQM SRIFQTLGAD PHNLGWFFIL PPLAGMLVQP 51 IVGHYSDRTW KPRLGGRRLP YLLYGTLIAV IVMILMPNSG SFGFGYASLA 101 ALSFGALMIA LLDVSSNMAM QPFKMMVGDM VNEEQKGYAY GIQSFLANTG 151 AWAAILPFV FAYIGLANTA EKGWPQTVV VAFYVGAALL VITSAFTIFK 201 VKEYDPETYA RYHGIDVAAN QEKANWIELL KTAPKAFWTV TLVQFFCWFA 251 FQYMWTYSAG AIAENVWHTT DASSVGYQEA GNWYGVLAAV QSVAAVICSF 301 VLAKVPNKYH KAGYFGCLAL GALGFFSVFF IGNQYALVLS YTLIGIAWAG 351 IITYPLTIVT NALSGKHMGT YLGLFNGSIC MPQIVASLLS FVLFPMLGGL 401 QATMFLVGGV VLLLGAFSVF LIKETHGGV* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ml43 / gl43 93.9% identity in 429 aa overlap 10 20 30 40 50 60 ml43. pep MLSFGFLGVQTAFTLQSSQMSRIFQTLGADPHNLGWFFILPPLAGMLVQPIVGHYSDRTW I I II I : I I I I I I I I I I I II I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I gl43 MLSFGYLGVQTAFTLQSSQMSRIFQTLGADPHNLGWFFILPPLAGMLVQPIVGYYSDRTW 10 20 30 40 50 60 70 80 90 100 110 120 ml43. pep KPRLGGRRLPYLLYGTLIAVIVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAM I II I I I I I I I I I I I I I I I I I I I I I II I I I I II I I I I I I I I I I I I I I I I I I I I I I II I I I I gl 43 KPRLGGRRLPYLLYGTLIAVIVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAM 70 80 90 100 110 120 130 140 150 160 170 180 ml 43 . pep QPFKMMVGDMVNEEQKGYAYGIQSFLANTGAWAAILPFVFAYIGLANTAEKGWPQTW I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl43 QPFKMMVGDMVNEEQKSYAYGIQSFLANTDAWAAILPFVFAYIGLANTAEKGWPQTW 130 140 150 160 170 180 190 200 210 220 230 240 ml 43 . pep VAFYVGAALLVITSAFTIFKVKEYDPETYARYHGIDVAANQEKANWIELLKTAPKAFWTV I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I : I I I I gl43 VAFYVGAALLIITSAFTISKVKEYDPETYARYHGIDVAANQEKANWFELLKTAPKVFWTV 190 200 210 220 230 240 250 260 270 280 290 300 ml43. pep TLVQFFCWFAFQYMWTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSF I I II I I I I II : I I I I I I I I I I I I I I I I I I I I I I I I: I I I I I lllllll lllllllll gl43 TPVQFFCWFAFRYMWTYSAGAIAENVWHTTDASSVGHQEAGNRYGVLAAVXSVAAVICSF 250 260 270 280 290 300 310 320 330 340 350 360 ml43. pep VLAKVPNKYHKAGYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVT : I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I 11111:111 I I I I I I I I I I I I I I I I I : gl43 ILAKVPNKYHKAGYFGCLALGALGFFSIFFIYNQYALILSYILIGIAWAGIITYPLTIVA 310 320 330 340 350 360 370 380 390 400 410 420 ml43. pep NALSGKHMGTYLGLFNGSICMPQIVASLLSFVLFPMLGGLQATMFLVGGWLLLGAFSVF llllllll I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I : I : I I I I I I I I I gl 3 NALSGKHMDTYLGLFNGSVCMPQIVASLLSFVLFPMLGGHQATMFLVAGAVLLLGAFSVC 370 380 390 400 410 420 430 ml 3 . pep LIKETHGGVX I I I I I I I I I gl43 LIKEIHGGVX 430 The following partial DNA sequence was identified in N meningitidis <SEQ LD 569>: al43. seq 1 ATGCTCAGTT TCGGCTTTCT CGGCGTTCAG ACGGCCTTTA CCCTGCAAAG 51 CTCGCAGATG AGCCGCATCT TCCAGACGCT CGGTGCCGAT CCGCACAGCC 101 TCGGCTGGTT CTTTATCCTG CCGCCGCTGG CGGGGATGCT GGTGCAGCCG 151 ATTGTCGGCC ATTACTCCGA CCGCACTTGG AAGCCGCGTT TGGGCGGCCG 201 CCGTCTGCCG TATCTGCTTT ATGGCACGCT GATTGCGGTT ATTGTGATGA 251 TTTTGATGCC GAACTCGGGC AGCTTCGGTT TCGGCTATGC GTCGCTGGCG 301 GCTTTGTCGT TCGGCGCGCT GATGATTGCG CTGTTAGACG TGTCGTCAAA 351 TATGGCGATG CAGCCGTTTA AGATGATGGT CGGCGACATG GTCAACGAGG 401 AGCAGAAAGG CTACGCCTAC GGGATTCAAA GTTTCTTAGC GAATACGGGC 451 GCGGTCGTGG CGGCGATTCT GCCGTTTGTG TTTGCGTATA TCGGTTTGGC 501 GAACACCGCC GAGAAAGGCG TTGTGCCGCA GACCGTGGTC GTGGCGTTTT 551 ATGTGGGTGC GGCGTTGCTG GTGATTACCA GCGCGTTCAC GATTTTCAAA 601 GTGAAGGAAT ACAATCCGGA AACCTACGCC CGTTACCACG GCATCGATGT 651 CGCCGCGAAT CAGGAAAAAG CCAACTGGAT CGAACTCTTG AAAACCGCGC 701 CTAAGGCGTT TTGGACGGTT ACTTTGGTGC AATTCTTCTG CTGGTTCGCC 751 TTCCAATATA TGTGGACTTA CTCGGCAGGC GCGATTGCGG AAAACGTCTG 801 GCACACCACC GATGCGTCTT CCGTAGGTTA TCAGGAGGCG GGTAACTGGT 851 ACGGCGTTTT GGCGGCGGTG CAGTCGGTTG CGGCGGTGAT TTGTTCGTTT 901 GTATTGGCGA AAGTGCCGAA TAAATACCAT AAGGCGGGTT ATTTCGGCTG 951 TTTGGCTTTG GGCGCGCTCG GCTTTTTCTC CGTTTTCTTC ATCGGCAACC 1001 AATACGCGCT GGTGTTGTCT TATACCTTAA TCGGCATCGC TTGGGCGGGC 1051 ATTATCACTT ATCCGCTGAC GATTGTGACC AACGCCTTGT CGGGCAAGCA 1101 TATGGGCACT TACTTGGGCC TGTTTAACGG CTCTATCTGT ATGCCGCAAA 1151 TCGTCGCTTC GCTGTTGAGT TTCGTGCTTT TCCCTATGCT GGGCGGCTTG 1201 CAGGCCACTA TGTTCTTGGT AGGGGGCGTC GTCCTGCTGC TGGGCGCGTT 1251 TTCCGTGTTC CTGATTAAAG AAACACACGG CGGGGTTTGA This corresponds to the amino acid sequence <SEQ ID 570; ORF 143. a>: al43.pep 1 MLSFGFLGVQ TAFTLQSSQM SRIFQTLGAD PHSLGWFFIL PPLAGMLVQP 51 IVGHYSDRTW KPRLGGRRLP YLLYGTLIAV IVMILMPNSG SFGFGYASLA 101 ALSFGALMIA LLDVSSNMAM QPFKMMVGDM VNEEQKGYAY GIQSFLANTG 151 AWAAILPFV FAYIGLANTA EKGWPQTVV VAFYVGAALL VITSAFTIFK 201 VKEYNPETYA RYHGIDVAAN QEKANWIELL KTAPKAFWTV TLVQFFCWFA 251 FQYMWTYSAG AIAENVWHTT DASSVGYQEA GNWYGVLAAV QSVAAVICSF 301 VLAKVPNKYH KAGYFGCLAL GALGFFSVFF IGNQYALVLS YTLIGIAWAG 351 IITYPLTIVT NALSGKHMGT YLGLFNGSIC MPQIVASLLS FVLFPMLGGL 401 QATMFLVGGV VLLLGAFSVF LIKETHGGV* ml43/al43 99.5% identity in 429 aa overlap 10 20 30 40 50 60 ml43. pep MLSFGFLGVQTAFTLQSSQMSRIFQTLGADPHNLGWFFILPPLAGMLVQPIVGHYSDRTW I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I II I I I I I II I I I I I I I I I I I I I I I I al43 MLSFGFLGVQTAFTLQSSQMSRIFQTLGADPHSLGWFFILPPLAGMLVQPIVGHYSDRTW 10 20 30 40 50 60 70 80 90 100 110 120 ml43.pep KPRLGGRRLPYLLYGTLIAVIVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAM I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al43 KPRLGGRRLPYLLYGTLIAVIVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAM 70 80 90 100 110 120 130 140 150 160 170 180 ml43.pep QPFKMMVGDMVNEEQKGYAYGIQSFLANTGAWAAILPFVFAYIGLANTAEKGWPQTW I I I I I I I I I I I I I I II I I I II I I I I I I I I I I I I I I I II I II I I I I I I I I I I I I I I I I I I I al43 QPFKMMVGDMVNEEQKGYAYGIQSFLANTGAWAAILPFVFAYIGLANTAEKGWPQTW 130 140 150 160 170 180 190 200 210 220 230 240 ml43.pep VAFYVGAALLVITSAFTIFKVKEYDPETYARYHGIDVAANQEKANWIELLKTAPKAFWTV I I I I I I I I I I I I I II I I I I I I I I I : I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I II al43 VAFYVGAALLVITSAFTIFKVKEYNPETYARYHGIDVAANQEKANWIELLKTAPKAFWTV 190 200 210 220 230 240 250 260 270 280 290 300 ml43.pep TLVQFFCWFAFQYMWTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSF I I I I I II I II I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I II I I I I I I al43 TLVQFFCWFAFQYMWTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSF 250 260 270 280 290 300 310 320 330 340 350 360 ml43.pep VLAKVPNKYHKAGYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVT I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I al43 VLAKVPNKYHKAGYFGCLALGALGFFΞVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVT 310 320 330 340 350 360 370 380 390 400 410 420 ml43.pep NALSGKHMGTYLGLFNGΞICMPQIVASLLSFVLFPMLGGLQATMFLVGGWLLLGAFSVF al43 NALSGKHMGTYLGLFNGSICMPQIVASLLSFVLFPMLGGLQATMFLVGGWLLLGAFSVF 370 380 390 400 410 420 430 ml43 . pep LIKETHGGVX I I I I I I I I I I al43 LIKETHGGVX 430 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ LD 571>: gl44. seq 1 ATGAGCGATA CCCCCGCTAC CCGCGATTTC GGCCTGATCG ACGGGCGGGC 51 CGTAACCGGC TATGTGCTGT CCAACCGGCG TGGTACGTGC GTCTTCGTGC 101 TGGACTTGGG CGGGATTGTG CAGGAATTTT CCGTTTTGGC AGACGGCGTG 151 CGCGAAAACC CCGTGGTGTC GTTCGACGAT GCGGCTTCCT ATGCGGACAA 201 TCCGTTTCAG ATTAACAAGC AGATAGGGCG CGTGGCCGGA CGCATCCGCG 251 GTGCGGCGTT CGACATCAAC GGTAGGACTT ACCGCGTGGA GGCCAACGAA 301 GGCAGGAACG CGCTGCACGG CGGTTCGCAC GGGCTGGCCG TTACCcgtTT 351 CAACGCGGTG GCGGCAGACG GccgacggTt atCCCAACGA TTTGGatatT 401 TCctaccgCT TGGACGAGGA CGGCCGGCTT ACCGTtaccT ATCGCGCCAC 451 CGCgctCGGC GACACGGTGT TCGACCCGAC GCTGCACATT TACTGGCGGC 501 TGGACGCGGG CCTGCACGAT GCGGTTCTGC ATATTCCGCA GGGCGGACAT 551 ATTCCGGCCG ATGCCGAAAA ACTGCCCGTC TTAACGGTTT CAGACGGCCT 601 CGAAGTATTT GA This corresponds to the amino acid sequence <SEQ ID 572; ORF 144.ng>: gl44.pep 1 MSDTPATRDF GLIDGRAVTG YVLSΝRRGTC VFVLDLGGIV QEFSVLADGV 51 REΝP SFDD AASYADΝPFQ IΝKQIGRVAG RIRGAAFDIΝ GRTYRVEAΝE 101 GRΝALHGGSH GLAVTRFΝAV AADGRRLSQR FGYFLPLGRG RPAYRYLSRH 151 RARRHGVRPD AAHLLAAGRG PARCGSAYSA GRTYSGRCRK TARLΝGFRRP 201 RSI* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 573>: ml44. seq 1 ATGAGCGATA CCCCCGCTAC CCGCGATTTC GGTCTGATCG ACGGGCGTGC 51 CGTAACCGGC TATGTGCTGT CCAACCGGCG TGGTACGCGT GTCTGCGTGC 101 TGGACTTGGG CGGGATTGTG CAGGAATTTT CCGTTTTGGC AGACGGCGTG 151 CGCGAAAACC TCGTGGTGTC GTTCGATGAT GCGGCTTCCT ATGCGGACAA 201 TCCGTTTCAG ATTAACAAAC AGATAGGGCG CGTGGCCGGA CGCATCCGCG 251 GTGCGGCGTT CGACATCAAC GGCAGGACTT ACCGCGTGGA GGCCAACGAA 301 GGCAGGAACG CGCTGCACGG CGGTTCGCAC GGGCTGGCCG TTACCCGTTT 351 CAACGCGGTG GCGGCAGACG GCCGTTCGGT GGTGCTGCGC AGCCGCCTGg 401 CAACAGTCGG CCGACGGTTA TCCCAACGAT TTGGATTTGG ATATTTCCTA 451 CCGCTTGGAC GAGGACGACC GGCTTACCGT TAcCTATCGC GCCACCGCGC 501 TCGGCGACAC GGTGTTCGAC CCGACGCTGC ACATTTACTG GCGGCTGGAC 551 GCGGGCCTGC ACGATGCGGT TCTGCATATT CCGCAGGGCG GACATATGCC 601 GGCCGATGCC GAAAAACTGC CCGTCTCAAC GGTTTCAGAC GACCTCGAAG 651 TATTTGA This corresponds to the amino acid sequence <SEQ ID 574; ORF 144>: ml44.pep 1 MSDTPATRDF GLIDGRAVTG YVLSΝRRGTR VCVLDLGGIV QEFSVLADGV 51 REΝLWSFDD AASYADΝPFQ IΝKQIGRVAG RIRGAAFDIΝ GRTYRVEAΝE 101 GRΝALHGGSH GLAVTRFΝAV AADGRSWLR SRLATVGRRL SQRFGFGYFL 151 PLGRGRPAYR YLSRHRARRH GVRPDAAHLL AAGRGPARCG SAYSAGRTYA 201 GRCRKTARLΝ GFRRPRSI* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ml44 / gl44 91.3% identity in 218 aa overlap 10 20 30 40 50 60 ml4 . pep MSDTPATRDFGLIDGRAVTGYVLSΝRRGTRVCVLDLGGIVQEFSVLADGVREΝLWSFDD I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I llllll gl44 MSDTPATRDFGLIDGRAVTGYVLSΝRRGTCVFVLDLGGIVQEFSVLADGVREΝPWSFDD 10 20 30 40 50 60 70 80 90 100 110 120 ml44. pep AASYADΝPFQIΝKQIGRVAGRIRGAAFDIΝGRTYRVEAΝEGRΝALHGGSHGLAVTRFΝAV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I gl 4 AASYADΝPFQIΝKQIGRVAGRIRGAAFDIΝGRTYRVEAΝEGRΝALHGGSHGLAVTRFΝAV 70 80 90 100 110 120 130 140 150 160 170 180 ml 44 . pep AADGRSWLRSRLATVGRRLSQRFGFGYFLPLGRGRPAYRYLSRHRARRHGVRPDAAHLL I I I l l l l l l l l l I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl44 AAD GRRLSQRFG— YFLPLGRGRPAYRYLSRHRARRHGVRPDAAHLL 130 140 150 160 190 200 210 219 ml44 . pep AAGRGPARCGSAYSAGRTYAGRCRKTARLΝGFRRPRSIX I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I gl44 AAGRGPARCGSAYSAGRTYSGRCRKTARLΝGFRRPRSIX 170 180 190 200 The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 575>: al44.seq 1 ATGAGCGATA CCCCCGCTAC CCGCGATTTC GGCCTGATCG ACGGGCGTGC 51 CGTAACCGGC TATGTGCTGT CCAACCGGCG TGGTACGCGT GTCTGCGTGC 101 TGGACTTGGG CGGGATTGTG CAGGAATTTT CCGTTTTGGC AGACGGCGTG 151 CGCGAAAACC TCGTGGTGTC GTTCGACGAT GCGGCTTCCT ATGCGGACAA 201 TCCGTTTCAG ATTAACAAGC AGATAGGGCG CGTGGCCGGA CGCATCCGCG 251 GTGCGGCGTT CGACATCAAC GGCAGGACTT ACCGCGTGGA GGCCAACGAA 301 GGCAGGAACG CGCTGCACGG CGGTTCGCAC GGGCTGGCCG TTACCCGTTT 351 CAACGCGGTG GCGGCAGACG GCCGTTCGGT GGTGCTGCGC AGCCGCCTG. 401 CAACAGTCGG CCGACGGTTA TCCCAACGAT TTGGATTTGG ATATTTCCTA 451 CCGCTTGGAC GAGGACGACC GGCTTACCGT TACCTATCGC GCCACCGCGC 501 TCGGCGACAC GGTGTTCGAC CCGACGCTGC ACATTTACTG GCGGCTGGAC 551 GCGGGCCTGC ACGATGCGGT TCTGCATATT CCGCAGGGCG GACATATTCC 601 GGCCGATGCC GAAAAACTGC CCGTCTCAAC GGTTTCAGAC GACCTCGAAG 651 TATTTGA This corresponds to the amino acid sequence <SEQ ID 576; ORF 144.a>: al44.pep 1 MSDTPATRDF GLIDGRAVTG YVLSΝRRGTR VCVLDLGGIV QEFSVLADGV 51 REΝLWSFDD AASYADΝPFQ IΝKQIGRVAG RIRGAAFDIΝ GRTYRVEAΝE 101 GRΝALHGGSH GLAVTRFΝAV AADGRSWLR SRLXTVGRRL SQRFGFGYFL 151 PLGRGRPAYR YLSRHRARRH GVRPDAAHLL AAGRGPARCG SAYSAGRTYS 201 GRCRKTARLΝ GFRRPRSI* ml44/al44 99.1% identity in 218 aa overlap 10 20 30 40 50 60 ml4 . pep MSDTPATRDFGLIDGRAVTGYVLSΝRRGTRVCVLDLGGIVQEFSVLADGVREΝLWSFDD I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al44 MSDTPATRDFGLIDGRAVTGYVLSΝRRGTRVCVLDLGGIVQEFSVLADGVREΝLWSFDD 10 20 30 40 50 60 70 80 90 100 110 120 ml44. pep AASYADΝPFQIΝKQIGRVAGRIRGAAFDIΝGRTYRVEAΝEGRΝALHGGSHGLAVTRFΝAV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al44 AASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEANEGRNALHGGSHGLAVTRFNAV 70 80 90 100 110 120 130 140 150 160 170 180 ml 44 . pep AADGRSWLRSRLATVGRRLSQRFGFGYFLPLGRGRPAYRYLSRHRARRHGVRPDAAHLL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al44 AADGRSWLRSRLXTVGRRLSQRFGFGYFLPLGRGRPAYRYLSRHRARRHGVRPDAAHLL 130 140 150 160 170 180 190 200 210 219 ml44 . pep AAGRGPARCGSAYSAGRTYAGRCRKTARLNGFRRPRSIX I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I al 44 AAGRGPARCGSAYSAGRTYSGRCRKTARLNGFRRPRSIX 190 200 210 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 577>: gl46. seq 1 ATGAAGCAAA TCCCCCTCCG CCTTCTCCAG GTCGTCATTG ACCACGACAA 51 AGTCGAACAA TACGGACTGT TCGATTTCAT GCCTTGCCTT CGACAGCCTC 101 CTTTGGATAa ctTCCCGACT GTCCGTCCCG CGCcctTTGA GGCGCGCGGC 151 AAGCACGTCG AAAGAAGGCG GCAGGATAAA GATACCGACA GCTTCCGGCA 201 GCGCGTTGCG AACCTGCGCC GCGCCCTGAA CGTCGATTTC CAAAATCACG 251 TCATAGCCTG CCGCCGCCAA CGCATTCACG CCCTCCGTGC TTGTGCCGTA 301 ATAGTTGCCG AATACGTCTG CGTATTCCAA AAAAGCCTCC TGCGCGATAA 351 GCGATTCAAA CTCTTCTTTG GAAACAAAGT GATAATGTAC GCCGTTTGCT 401 TCGCCTTCAC GCGGCGGGCG CGTCGTATGC GACACGGAAA CGCGCAAACC 451 GTTATGGTTT GCCAACAGCC GCGACACCAG CGTGGTTTTG CCCGTGCCGG 501 AAGCGGCCGA AATGATAAAG ATGTTGCCTT TTCGATAAGC GGACATATTT 551 TTTACCTGTA TATTTTCCAA CCGATTGTAT CACAACGGAC ACCCTATTTC 601 ATATTTGCCG ATGCCCATAT TTTGCCGCTA TTGTTTTGA This corresponds to the amino acid sequence <SEQ ID 578; ORF 146.ng>: gl46.pep 1 MKQIPLRLLQ WIDHDKVEQ YGLFDFMPCL RQPPLDΝFPT VRPAPFEARG 51 KHVERRRQDK DTDSFRQRVA ΝLRRALΝVDF QΝHVIACRRQ RIHALRACAV 101 IVAEYVCVFQ KSLLRDKRFK LFFGΝKVIMY AVCFAFTRRA RRMRHGΝAQT 151 VMVCQQPRHQ RGFARAGSGR ΝDKDVAFSIS GHIFYLYIFQ PIVSQRTPYF 201 IFADAHILPL LF* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 579>: ml46. seq 1 ATGGCGCAAA TCCTCCTCCG CTCGCGCCAA GTCGTCATTG ACCACGACAA 51 AGTCAAACAA TACGGACTGC TCGATTTCAT GCCTTGCCTT CGACAGCCTC 101 CTTTGGATAA CTTCCCGACT GTCCGTCCCG CGTCCGTTGA GGCGCGCGGC 151 AAGTACGTCG AAAGAAGGCG GCAGGATAAA GATGCCGACG GCTTCGGGCA 201 GCGCGTCGCG AACCTGCGCC GCGCCCTGAA CGTCGATTTC CAAAATCACG 251 TCATAGCCTG CCGCCGCCAA CGCATTCACA CCCTCCGCGC CTGTGCCGTA 301 ATAGTTGCCA AATACGTCGG CGTATTCCAA AAAAGCTTCC TGCGCGATAA 351 GCGACTCAAA CTCTTCTTTG GAAACAAAGT GATAATGTAC GCCGTTTGCT 401 TCGCCTTCAC GCGGCGGGCG CGTCGTGTGC GACACGGAAA CGCGCAAACC 451 GTTATGGTTT GCCAACAGCC GCGACACCAG CGTGGTTTTG CCCGTGCCGG 501 AAGCGGCCGA AATGATAAAG ATGTTGCCTT TTCGATAAGC GGACATATTT 551 TTTACCTGTA TATTTTCCAG CCGATTGTAT CACAATGGAC ACCCAGTTTC 601 CTATTTGCCG ATGCCCATAT TTTGCCGCTA TTGTTTTGA This corresponds to the amino acid sequence <SEQ ID 580; ORF 146>: ml46. pep 1 MAQILLRSRQ WIDHDKVKQ YGLLDFMPCL RQPPLDΝFPT VRPASVEARG 51 KYVERRRQDK DADGFGQRVA ΝLRRALΝVDF QΝHVIACRRQ RIHTLRACAV 101 IVAKYVGVFQ KSFLRDKRLK LFFGΝKVIMY AVCFAFTRRA RRVRHGΝAQT 151 VMVCQQPRHQ RGFARAGSGR ΝDKDVAFSIS GHIFYLYIFQ PIVSQWTPSF 201 LFADAHILPL LF* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ml46 / gl46 90.1% identity in 212 aa overlap 10 20 30 40 50 60 ml46. pep MAQILLRSRQWIDHDKVKQYGLLDFMPCLRQPPLDΝFPTVRPASVEARGKYVERRRQDK I II II I I I I I I I I I : I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I gl46 MKQIPLRLLQWIDHDKVEQYGLFDFMPCLRQPPLDΝFPTVRPAPFEARGKHVERRRQDK 10 20 30 40 50 60 70 80 90 100 110 120 ml46. pep DADGFGQRVAΝLRRALΝVDFQΝHVIACRRQRIHTLRACAVIVAKYVGVFQKSFLRDKRLK I: h I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I : I I I I I I I: I I I I I: I gl46 DTDSFRQRVAΝLRRALΝVDFQΝHVIACRRQRIHALRACAVIVAEYVCVFQKSLLRDKRFK 70 80 90 100 110 120 130 140 150 160 170 180 ml46. pep LFFGΝKVIMYAVCFAFTRRARRVRHGΝAQTVMVCQQPRHQRGFARAGSGRΝDKDVAFSIS I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl46 LFFGΝKVIMYAVCFAFTRRARRMRHGΝAQTVMVCQQPRHQRGFARAGSGRΝDKDVAFSIS 130 140 150 160 170 180 190 200 210 ml46.pep GHIFYLYIFQPIVSQWTPSFLFADAHILPLLFX lllllllllllllll II I: I I I I I I I I I I I I gl46 GHIFYLYIFQPIVSQRTPYFIFADAHILPLLFX 190 200 210 The following partial DΝA sequence was identified in N meningitidis <SEQ LD 581>: al46. seq 1 ATGGCGCAAA TCCTCCTCCG CCCGCGCCAA GTCATCATTG ACCACGACAA 51 AATCGAACAA TACGGACTGT TCGATTTCAT GCCTTGCCTT CGACAGCCTC 101 CTTTGGATAA CTTCCCGACT GTCCGTCCCG CGTCCGTTGA GACGCGCAGC 151 AAGCACATCG AAAGACGGCG GCAGGATAAA GATGCCGACG GCTTCGGGCA 201 GCGCATCTCG AACCTGAGCC GCGCCCTGAA CGTCGATTTC CAAAATCACG 251 TCATAACCTG CCGCCGCCAA CGCATTCACA CCCTCCGCGC TTGTGCCGTA 301 ATAGTTGCCG AACACGTCCG CGTATTCCAA AAAAGCCTCC TGCGCGATAA 351 GCGACTCAAA CTCTTCTTTG GAAACAAAGT GATAATGTAC GCCGTTTGCT 401 TCGCCTTCAC GCGGCGGACG CGTCGTGTGC GACACGGAAA CGCGCAAACC 451 GTTATGGTTT GCCAACAGCC GCGACACCAG CGTGGTTTTG CCCGTGCCGG 501 AAGCGGCCGA AATGATAAAG ATGTTGCCTT TTCGATAAGC GGACATATTT 551 TTTACCTGTA TATTTTCCAG CCGATTGTAT CACAACGGAC ACCCGGTTTC 601 CTATTTGCCG ATGCCCATAT TTTGCCGCTA TTGTTTTGA This corresponds to the amino acid sequence <SEQ ID 582; ORF 146.a>: al46.pep 1 MAQILLRPRQ VIIDHDKIEQ YGLFDFMPCL RQPPLDΝFPT VRPASVETRS 51 KHIERRRQDK DADGFGQRIS ΝLSRALΝVDF QΝHVITCRRQ RIHTLRACAV 101 IVAEHVRVFQ KSLLRDKRLK LFFGΝKVIMY AVCFAFTRRT RRVRHGΝAQT 151 VMVCQQPRHQ RGFARAGSGR ΝDKDVAFSIS GHIFYLYIFQ PIVSQRTPGF 201 LFADAHILPL LF* ml46/al46 90.6% identity in 212 aa overlap 10 20 30 40 50 60 ml46. pep MAQILLRSRQWIDHDKVKQYGLLDFMPCLRQPPLDΝFPTVRPASVEARGKYVERRRQDK lllllll I II: I I I I I:: I I I I : I I I I I I I I I I I I I I I I I M I I I I : I : I:: I I I I I II al46 MAQILLRPRQVIIDHDKIEQYGLFDFMPCLRQPPLDΝFPTVRPASVETRSKHIERRRQDK 10 20 30 40 50 60 70 80 90 100 110 120 ml46. pep DADGFGQRVANLRRALNVDFQNHVIACRRQRIHTLRACAVIVAKYVGVFQKSFLRDKRLK II I I II I I :: II I I I I I II I I I I I : I I I I I I I II I I I I I I I I:: I I I I I I: I I I I I I I al46 DADGFGQRISNLSRALNVDFQNHVITCRRQRIHTLRACAVIVAEHVRVFQKSLLRDKRLK 70 80 90 100 110 120 130 140 150 160 170 180 ml46. pep LFFGNKVIMYAVCFAFTRRARRVRHGNAQTVMVCQQPRHQRGFARAGSGRNDKDVAFSIS I I II I I I I I I I I I I I I I I I : I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I al 6 LFFGNKVIMYAVCFAFTRRTRRVRHGNAQTVMVCQQPRHQRGFARAGSGRNDKDVAFSIS 130 140 150 160 170 180 190 200 210 ml46.pep GHIFYLYIFQPIVSQWTPSFLFADAHILPLLFX I I : I I I I II I I I I I I I I al46 GHIFYLYIFQPIVSQRTPGFLFADAHILPLLFX 190 200 210 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 583>: gl47.seq (partial) 1 ..ATGCGACGAG AAGCCAAAAT GGCACAAATC ACACTCAAAC CCATTGTTTT 51 ATCAATTCTT TTAATCAACA CACCCCTCCT CGCCCAAGCG CATGAAACTG 101 AGCAATCGGT GGGCTTGGAA ACGGTCAGCG TCGTCGGCAA AAGCCGTCCG 151 CGCGCGACTT CGGGGCTGCT GCACACTTCG ACCGCCTCCG ACAAAATCAT 201 CTCCGGCGAT ACTTTGCGCC AAAAAGCCGT CAACTTGGGC GACGCTTTGG 251 ACGGCGTACC GGGCATCCAC GCTTCGCAAT ACGGCGGCGG CGCATCCGCT 301 CCCGTTATTC GCGGTCAAAC GGGCAGACGG ATTAAAGTAT TGAACCATCA 351 CGGCGAAACG GGCGATATGG CGGACTTTTC TCCCGATCAC GCCATTATGG 401 TAGATACCGC CTTGTCGCAA CAGGTTGAAA TCCTGCGCGG GCCGGTTACG 451 CTCTTGTACA GCTCGGgcaa tgtggccgGG GCTGGtcaat gttgccgatg 501 gAAAAAtccc ccaaaaAAtg cc. This corresponds to the amino acid sequence <SEQ ID 584; ORF 147.ng>: gl47.pep (partial) 1 ..MRREAKMAQI TLKPIVLSIL LIΝTPLLAQA HETEQSVGLE TVSWGKSRP 51 RATSGLLHTS TASDKIISGD TLRQKAVΝLG DALDGVPGIH ASQYGGGASA 101 PVIRGQTGRR IKVLΝHHGET GDMADFSPDH AIMVDTALSQ QVEILRGPVT 151 LLYSSGΝVAG AGQCCRWKΝP PKΝA.. The following partial DΝA sequence was identified in N meningitidis <SEQ ID 585>: ml47.seq (partial) 1 .. CCGCATAAAA CTGAGCAATC GGTGGATTTG GAAACGGTCA GCGTCGTCGG 51 CAAAAGCCGT CCGCGCGCCA CGTCGGGGCT GTTGCACACT TCGACCGCCT 101 CCGACAAAAT CATCTCCGGC GATACCTTGC GCCAAAAAGC CGTCAACTTG 151 GGCGACGCTT TAGACGGCGT ACCGGGCATC CACGCTTCGC AATACGGCGG 201 CGGCGCGTCT GCTCCCGTCA TTCGCGGTCA AACAGGCAGG CGGATTAAAG 251 TGTTGAACCA TCACGGCGAA ACAGGCGATA TGGCGGATTT TTCGCCCGAT 301 CACGCCATTA TGGTAGATAC CGCCTTGTCG CAACAGGTCG AAATCCTGCG 351 CGGGCCGGTT ACGCTCTTGT ACAGCTCGGG CAATGTGGCG GGGCTGGTCG 401 ATGTTGCCGA TGGCAAAATC CCCGAAAAAA TGCCTGAAAA CGGCGTATCG 451 GGCGAACTCG GATTGCGTTT GAGCAGCGGC AATCTGGAAA AACTCACGTC 501 CGGCGGCATC AATATCGGTT TGGGCAAAAA CTTTGTATTG CACACGGAAG 551 GGCTGTACCG CAAATCGGGG GATTACGCCG TACCGCGTTA CCGCAATCTG 601 AAACGCCTGC CCGACAGCCA CGCCGATTCG CAAACGGGCA GCATCGGGCT 651 GTCTTGGGTT GGCGAAAAAG GTTTTATCGG CGTAGCGTAC AGCGACCGTC 701 GCGACCAATA TGGTCTGCCT GCCCACAGCC ACGAATACGA TGATTGCCAC 751 GCCGACATCA TCTGGCAAAA GAGCTTGATT AACAAACGCT ATTTACAGCT 801 TTATCCGCAC CTGTTGACCG AAGAAGACAT CGATTACGAC AATCCGGGCT 851 TGAGCTGCGG CTTCCACGAC GACGATAATG CACACGCACA CACCCACAGC 901 GGCAGACCGT GGATAGACCT GCGCAACAAA CGCTACGAAC TCCGTGCCGA 951 ATGGAAGCAA CCGTTCCCCG GTTTTGAAGC CCTGCGCGTA CACCTGAACC 1001 GCAACGACTA CCGCCACGAC GAAAAAGCAG GCGATGCAGT CGAAAACTTT 1051 TTTAACAACC AAACGCAAAA CGCCCGCATC GAGTTGCGCC ACCAACCCAT 1101 AGGTCGTCTG AAAGGCAGCT GGGGCGTGCA ATATTTACAA CAAAAATCCA 1151 GTGCTTTATC TGCCATATCC GAAGCGGTTA AACAACCGAT GCTGCTTGAC 1201 AACAAAGTGC AACATTACAG CTTTTTCGGT GTAGAACAGG CAAACTGGGA 1251 CAACTTCACG CTTGAAGGAG GCGTACGCGT GGAAAAACAA AAAGCCTCCA 1301 TTCAGTACGA CAAAGCATTG ATTGATCGGG AAAACTACTA CAACCACCCC 1351 CTGCCCGACC TCGGCGCGCA CCGCCAAACC GCCCGCTCAT TCGCACTTTC 1401 GGGCAACTGG TATTTCACGC CACAACACAA ACTCAGCCTG ACCGCCTCCC 1451 ATCAGGAACG CCTGCCGTCA ACGCAAGAGC TGTACGCACA CGGCAAACAC 1501 GTCGCCACCA ACACCTTTGA AGTCGGCAAC AAACACCTCA ACAAAGAGCG 1551 TTCCAACAAT ATCGAACTCG CGCTGGGCTA CGAAGGCGAC CGCTGGCAAT 1601 ACAATCTGGC ACTCTACCGC AACCGCTTCG GTAACTACAT TTACGCCCAA 1651 ACCTTAAACG ACGGACGCGG CCCCAAATCC ATCGAAGACG ACAGCGAAAT 1701 GAAGCTCGTG CGCTACAACC AATCCGGCGC CGACTTCTAC GGCGCGGAAG 1751 GCGAAATCTA CTTCAAACCG ACACCGCGCT ACCGCATCGG CGTTTCCGGC 1801 GACTATGTAC GAGGCCGTCT GAAAAACCTG CCTTCCCTAC CCGGCAGAGA 1851 AGATGCCTAC GGCAACCGTC CTTTCATCGC ACAGGACGAC CAAAATGCCC 1901 CCCGTGTTCC GGCTGCGCGC CTCGGCTTCC ACCTGAAAGC CTCGCTGACC 1951 GACCGTATCG ATGCCAATTT GGACTACTAC CGCGTGTTCG CCCAAAACAA 2001 ACTCGCCCGC TACGAAACGC GCACGCCCGG ACACCATATG CTCAACCTCG 2051 GCGCAAACTA CCGCCGCAAT ACGCGCTATG GCGAGTGGAA TTGGTACGTC 2101 AAAGCCGACA ACCTGCTCAA CCAATCCGTT TACGCCCACA GCAGCTTTCT 2151 CTCTGATACG CCGCAAATGG GCCGCAGCTT TACCGGCGGC GTGAACGTGA 2201 AGTTTTAA This corresponds to the amino acid sequence <SEQ ID 586; ORF 147>: ml47 .pβp (partial ) 1 .. PHKTEQSVDL ETVSVVGKSR PRATSGLLHT STASDKIISG DTLRQKAVNL 51 GDALDGVPGI HASQYGGGAS APVIRGQTGR RIKVLNHHGE TGDMADFSPD 101 HAIMVDTALS QQVEILRGPV TLLYSSGNVA GLVDVADGKI PEKMPENGVS 151 GELGLRLSSG NLEKLTSGGI NIGLGKNFVL HTEGLYRKSG DYAVPRYRNL 201 KRLPDSHADS QTGSIGLSWV GEKGFIGVAY SDRRDQYGLP AHSHEYDDCH 251 ADIIWQKSLI NKRYLQLYPH LLTEEDIDYD NPGLSCGFHD DDNAHAHTHS 301 GRPWIDLRNK RYELRAEWKQ PFPGFEALRV HLNRNDYRHD EKAGDAVENF 351 FNNQTQNARI ELRHQPIGRL KGSWGVQYLQ QKSSALSAIS EAVKQPMLLD 401 NKVQHYSFFG VEQANWDNFT LEGGVRVEKQ KASIQYDKAL IDRENYYNHP 451 LPDLGAHRQT ARSFALSGNW YFTPQHKLSL TASHQERLPS TQELYAHGKH 501 VATNTFEVGN KHLNKERSNN IELALGYEGD RWQYNLALYR NRFGNYIYAQ 551 TLNDGRGPKS IEDDSEMKLV RYNQSGADFY GAEGEIYFKP TPRYRIGVSG 601 DYVRGRLKNL PSLPGREDAY GNRPFIAQDD QNAPRVPAAR LGFHLKASLT 651 DRIDANLDYY RVFAQNKLAR YETRTPGHHM LNLGANYRRN TRYGEWNWYV 701 KADNLLNQSV YAHSSFLSDT PQMGRSFTGG VNVKF* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ml47 / gl47 92.3% identity in 142 aa overlap 10 20 30 ml47.pep PHKTEQSVDLETVSWGKSRPRATSGLLHTS 1:11111 I I I I I I I I I I I I I I I I I I I I I I gl47 MRREAKMAQITLKPIVLSILLIΝTPLLAQAHETEQSVGLETVSWGKSRPRATSGLLHTS 10 20 30 40 50 60 40 50 60 70 80 90 ml47.pep TASDKIISGDTLRQKAVΝLGDALDGVPGIHASQYGGGASAPVIRGQTGRRIKVLΝHHGET I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I II II I I I I I I I I I I II I I I I I I gl47 TASDKIISGDTLRQKAVΝLGDALDGVPGIHASQYGGGASAPVIRGQTGRRIKVLΝHHGET 70 80 90 100 110 120 100 110 120 130 140 150 ml47.pep GDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGΝVAGLVDVADGKIPEKMPEΝGVSG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I gl47 GDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGΝVAGAGQCCRWKΝPPKΝA The following partial DNA sequence was identified inN meningitidis <SEQ LD 587>: al47.seq 1 ATGCGACGAG AAGCCAAAAT GGCACAAACT ACACTCAAAC CCATTGTTTT 51 ATCAATTCTT TTAATCAACA CACCCCTCCT CTCCCAAGCG CATGGAACTG 101 AGCAATCAGT GGGCTTGGAA ACGGTCAGCG TCGTCGGCAA AAGCCGTCCG 151 CGCGCCACTT CGGGGCTGCT GCACACTTCT ACCGCCTCCG ACAAAATCAT 201 CAGCGGCGAC ACCTTGCGAC AAAAAGCCGT CAACTTGGGT GATGCTTTAG 251 ACGGCGTACC GGGCATTCAT GCCTCGCAAT ACGGCGGCGG CGCATCCGCT 301 CCCGTTATTC GCGGTCAAAC AGGCAGACGG ATTAAAGTGT TGAACCATCA 351 CGGCGAAACG GGCGACATGG CGGACTTCTC TCCAGACCAT GCAATCATGG 401 TGGACAGCGC CTTGTCGCAA CAGGTCGAAA TCCTGCGCGG TCCGGTTACG 451 CTCTTGTACA GCTCGGGCAA TGTGGCGGGG CTGGTCGATG TTGCCGATGG 501 CAAAATCCCC GAAAAAATGC CTGAAAACGG CGTATCGGGC GAACTCGGAT 551 TGCGTTTGAG CAGCGGCAAT CTGGAAAAAC TCACGTCCGG CGGCATCAAT 601 ATCGGTTTGG GCAAAAACTT TGTATTGCAC ACGGAAGGGC TGTACCGCAA 651 ATCGGGGGAT TACGCCGTAC CGCGTTACCG CAATCTGAAA CGCCTGCCCG 701 ACAGCCACGC CGATTCGCAA ACGGGCAGCA TCGGGCTGTC TTGGGTTGGC 751 GAAAAAGGCT TTATCGGCGC AGCATACAGC GACCGTCGCG ACCAATATGG 801 TCTGCCTGCC CACAGCCACG AATACGATGA TTGCCACGCC GACATCATCT 851 GGCAAAAGAG TTTGATTAAC AAACGCTATT TGCAGCTTTA TCCGCACCTG 901 TTGACCGAAG AAGACATCGA TTACGACAAT CCGGGCTTGA GCTGCGGCTT 951 TCACGACGAC GATGATGCAC ACGCCCATGC CCACAACGGC AAACCTTGGA 1001 TAGACCTGCG CAACAAACGC TACGAACTCC GCGCCGAATG GAAGCAACCG 1051 TTCCCCGGTT TTGAAGCCCT GCGCGTACAC CTGAACCGCA ACGACTACCG 1101 CCACGACGAA AAAGCAGGCG ATGCAGTAGA AAACTTTTTT AACAACCAAA 1151 CGCAAAACGC CCGTATCGAG TTGCGCCACC AACCCATAGG CCGTCTGAAA 1201 GGCAGCTGGG GCGTGCAATA TTTGGGACAA AAATCCAGTG CTTTATCTGC 1251 CACATCCGAA GCGGTCAAAC AACCGATGCT GCTTGACAAT AAAGTGCAAC 1301 ATTACAGCTT TTTCGGTGTA GAACAGGCAA ACTGGGACAA CTTCACGCTT 1351 GAAGGCGGCG TACGCGTGGA AAAACAAAAA GCCTCCATCC GCTACGACAA 1401 AGCATTGATT GATCGGGAAA ACTACTACAA CCATCCCCTG CCCGACCTCG 1451 GCGCGCACCG CCAAACCGCC CGCTCATTCG CACTTTCGGG CAACTGGTAT 1501 TTCACGCCAC AACACAAACT CAGCCTGACC GCCTCCCATC AGGAACGCCT 1551 GCCGTCAACG CAAGAGCTGT ACGCACACGG CAAACACGTC GCCACCAACA 1601 CCTTTGAAGT CGGCAACAAA CACCTCAACA AAGAGCGTTC CAACAATATC 1651 GAACTCGCGC TGGGCTACGA AGGCGACCGC TGGCAATACA ATCTGGCACT 1701 CTACCGCAAC CGCTTCGGCA ACTACATTTA CGCCCAAACC TTAAACGACG 1751 GACGCGGCCC CAAATCCATC GAAGACGACA GCGAAATGAA GCTCGTGCGC 1801 TACAACCAAT CCGGTGCGGA CTTCTACGGC GCGGAAGGCG AAATCTACTT 1851 CAAACCGACA CCGCGCTACC GCATCGGCGT TTCCGGCGAC TATGTACGAG 1901 GCCGTCTGAA AAACCTGCCT TCCCTACCCG GCAGGGAAGA CGCCTACGGC 1951 AACCGCCCAC TCATTGCCCA AGCCGACCAA AACGCCCCTC GCGTTCCGGC 2001 TGCGCGCCTC GGCGTCCACC TGAAAGCCTC GCTGACCGAC CGCATCGATG 2051 CCAATTTGGA CTACTACCGC GTGTTCGCCC AAAACAAACT CGCCCGCTAC 2101 GAAACGCGCA CGCCCGGACA CCATATGCTC AACCTCGGCG CAAACTACCG 2151 CCGCAATACG CGCTATGGCG AGTGGAATTG GTACGTCAAA GCCGACAACC 2201 TGCTCAACCA ATCCGTTTAC GCCCACAGCA GCTTCCTCTC TGATACGCCG 2251 CAAATGGGCC GCAGCTTTAC CGGCGGCGTG AACGTGAAGT TTTAA This corresponds to the amino acid sequence <SEQ ID 588; ORF 147.a>: al47.pep 1 MRREAKMAQT TLKPIVLSIL LIΝTPLLSQA HGTEQSVGLE TVSWGKSRP 51 RATSGLLHTS TASDKIISGD TLRQKAVΝLG DALDGVPGIH ASQYGGGASA 101 PVIRGQTGRR IKVLΝHHGET GDMADFSPDH AIMVDSALSQ QVEILRGPVT 151 LLYSSGΝVAG LVDVADGKIP EKMPEΝGVSG ELGLRLSSGΝ LEKLTSGGIΝ 201 IGLGKΝFVLH TEGLYRKSGD YAVPRYRΝLK RLPDSHADSQ TGSIGLSWVG 251 EKGFIGAAYS DRRDQYGLPA HSHEYDDCHA DIIWQKSLIΝ KRYLQLYPHL 301 LTEEDIDYDΝ PGLSCGFHDD DDAHAHAHΝG KPWIDLRΝKR YELRAEWKQP 351 FPGFEALRVH LΝRΝDYRHDE KAGDAVEΝFF ΝΝQTQΝARIE LRHQPIGRLK 401 GSWGVQYLGQ KSSALSATSE AVKQPMLLDΝ KVQHYSFFGV EQAΝWDΝFTL 451 EGGVRVEKQK ASIRYDKALI DREΝYYΝHPL PDLGAHRQTA RSFALSGΝWY 501 FTPQHKLSLT ASHQERLPST QELYAHGKHV ATΝTFEVGΝK HLΝKERSΝΝI 551 ELALGYEGDR WQYΝLALYRΝ RFGΝYIYAQT LΝDGRGPKSI EDDSEMKLVR 601 YNQSGADFYG AEGEIYFKPT PRYRIGVSGD YVRGRLKNLP SLPGREDAYG 651 NRPLIAQADQ NAPRVPAARL GVHLKASLTD RIDANLDYYR VFAQNKLARY 701 ETRTPGHHML NLGANYRRNT RYGEWNWYVK ADNLLNQSVY AHSSFLSDTP 751 QMGRSFTGGV NVKF* ml47/al47 98.1% identity in 734 aa overlap 10 20 30 ml 47. pep PHKTEQSVDLETVSWGKSRPRATSGLLHTS I I I I II I I I I I I I I I I I I I II I I I I I I I al47 MRREAKMAQTTLKPIVLSILLINTPLLSQAHGTEQSVGLETVSWGKSRPRATSGLLHTS 10 20 30 40 50 60 40 50 60 70 80 90 ml47. pep TASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTGRRIKVLNHHGET I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I al47 TASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTGRRIKVLNHHGET 70 80 90 100 110 120 100 110 120 130 140 150 ml47. pep GDMADFSPDHAIMVDTALΞQQVEILRGPVTLLYSSGNVAGLVDVADGKIPEKMPENGVSG I I I I I I I I I I I I I I I: I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al47 GDMADFSPDHAIMVDSALSQQVEILRGPVTLLYSSGNVAGLVDVADGKIPEKMPENGVSG 130 140 150 160 170 180 160 170 180 190 200 210 ml47. pep ELGLRLSSGNLEKLTSGGINIGLGKNFVLHTEGLYRKSGDYAVPRYRNLKRLPDSHADSQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I al47 ELGLRLSSGNLEKLTSGGINIGLGKNFVLHTEGLYRKSGDYAVPRYRNLKRLPDSHADSQ 190 200 210 220 230 240 220 230 240 250 260 270 ml47. pep TGSIGLSWVGEKGFIGVAYSDRRDQYGLPAHSHEYDDCHADIIWQKSLINKRYLQLYPHL I I I I I I I I I I I I I I I I : I I I I I I I I II I I I I I II I I I I I I I I I I I I I I I I I I I I I II I I I al47 TGSIGLSWVGEKGFIGAAYSDRRDQYGLPAHSHEYDDCHADIIWQKSLINKRYLQLYPHL 250 260 270 280 290 300 280 290 300 310 320 330 ml47. pep LTEEDIDYDNPGLSCGFHDDDNAHAHTHSGRPWIDLRNKRYELRAEWKQPFPGFEALRVH I I II I I I I I I I I I I I I I I I I I : I I I I : I : I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al47 LTEEDIDYDNPGLSCGFHDDDDAHAHAHNGKPWIDLRNKRYELRAEWKQPFPGFEALRVH 310 320 330 340 350 360 340 350 360 370 380 390 ml47. pep LNRNDYRHDEKAGDAVENFFNNQTQNARIELRHQPIGRLKGSWGVQYLQQKSSALSAISE I I I I I I I I I I I I I I II I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I llllllll II al 47 LNRNDYRHDEKAGDAVENFFNNQTQNARIELRHQPIGRLKGSWGVQYLGQKSSALSATSE 370 380 390 400 410 420 400 410 420 430 440 450 ml 47. pep AVKQPMLLDNKVQHYSFFGVEQANWDNFTLEGGVRVEKQKASIQYDKALIDRENYYNHPL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I II I I I I I al47 AVKQPMLLDNKVQHYSFFGVEQANWDNFTLEGGVRVEKQKASIRYDKALIDRENYYNHPL 430 440 450 460 470 480 460 470 480 490 500 510 ml 47. pep PDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQERLPSTQELYAHGKHVATNTFEVGNK I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I al47 PDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQERLPSTQELYAHGKHVATNTFEVGNK 490 500 510 520 530 540 520 530 540 550 560 570 ml47. pep HLNKERSNNIELALGYEGDRWQYNLALYRNRFGNYIYAQTLNDGRGPKSIEDDSEMKLVR I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I i I I I I I I I I I I I I al47 HLNKERSNNIELALGYEGDRWQYNLALYRNRFGNYIYAQTLNDGRGPKSIEDDSEMKLVR 550 560 570 580 590 600 580 590 600 610 620 630 ml 7. pep YNQSGADFYGAEGEIYFKPTPRYRIGVSGDYVRGRLKNLPSLPGREDAYGNRPFIAQDDQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I II al47 YNQSGADFYGAEGEIYFKPTPRYRIGVSGDYVRGRLKNLPSLPGREDAYGNRPLIAQADQ 610 620 630 640 650 660 640 650 660 670 680 690 ml47.pep NAPRVPAARLGFHLKASLTDRIDANLDYYRVFAQNKLARYETRTPGHHMLNLGANYRRNT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I al47 NAPRVPAARLGVHLKASLTDRIDANLDYYRVFAQNKLARYETRTPGHHMLNLGANYRRNT 670 680 690 700 710 720 700 710 720 730 ml47. pep RYGEWNWYVKADNLLNQSVYAHSSFLSDTPQMGRSFTGGVNVKFX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I al47 RYGEWNWYVKADNLLNQSVYAHSΞFLSDTPQMGRSFTGGVNVKFX 730 740 750 760 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 589>: gl48. seq 1 ATGGCGTTAA AAACATCAAA CTTGGAACAC GCAATGctgg ttcaTCCCGA 51 AgctATgagt gtcggcgCGC TTGccgAcaa AATCCGCAAA AtcgaAAact 101 gGCCGCAAAA AGgcaTCTTA TTCCACGACA TCACGCCCGT CCTGCAAAGT 151 GCGGAATACT TCCGCCTTTT GGTCGATTTG CTGGTTTACC GCTATATGGA 201 TCAGAAAATC GACATCGTTG CCGGCTTGGA CGCGCGCGGC TTCATTATCG 251 GCGCGGCACT CGCCTACCAG CTCAaCGtcg gctTCGTCCC CATCCGCAAA 301 AAAGGCAAGC TGCCTTTTGA AACCGTATCG CAAAGCTAcg cgcTCGAATA 351 CGGGGAAGCT GCGGTGGAAA TCCACACCGa tgccgTCAAA CCCGGTTCGC 401 GCGTCCTGCT GGTCGATGAT TTGGTTGCCA CGGGCGGCAC AATGCTTGCC 451 GGGCTGGAAC TGATCCGCAA ACTCGGCGGG GAAATTGTCG AAgccgccgC 501 CATTTTGGAA TTTACCGACC TTCAAGGCGG CAAGAATATC CGCGCAAGTG 551 GCGCGCCCTT ATTTACCCTG CTTCAAAACG AAGGCTGCAT GAAAGGCTGA This corresponds to the amino acid sequence <SEQ ID 590; ORF 148.ng>: gl48.pep 1 MALKTSΝLEH AMLVHPEAMS VGALADKIRK IEΝWPQKGIL FHDITPVLQS 51 AEYFRLLVDL LVYRYMDQKI DIVAGLDARG FIIGAALAYQ LΝVGFVPIRK 101 KGKLPFETVS QSYALEYGEA AVEIHTDAVK PGSRVLLVDD LVATGGTMLA 151 GLELIRKLGG EIVEAAAILE FTDLQGGKΝI RASGAPLFTL LQΝEGCMKG* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 591>: ml 48. seq 1 ATGGCGTTAA AAACATCAAA CTTGGAACAC GCAATGCTGG TTCATCCCGA 51 AGCTATGAGT GTCGGCGCGC TTGCCGACAA AATCCGCAAA ATCGAAAACT 101 GGCCGCAAAA AGGCATCTTA TTCCACGACA TCACGCCCGT CCTTCAAAGC 151 GCGGAATACT TCCGCCTTTT GGTTGATTTA TTGGTTTACC GCTATATGGA 201 TCAGAAAATC GACATCGTTG CCGGTTTGGA CGCGCGCGGC TTCATTATCG 251 GCGCGGCACT CGCCTACCAG CTCAACGTCG GTTTCGTCCC CATCCGCAAA 301 AAAGGCAAGC TGCCTTTTGA AACCGTATCG CAAAGCTACG CGCTCGAATA 351 CGGGGAAGCT GCGGTGGAAA TCCACACCGA TGCCGTCAAA CTCGGTTCGC 401 GCGTGCTGCT GGTCGATGAT TTGATTGCCA CGGGCGGCAC GATGCTTGCC 451 GGACTGGAAC TGATCCGCAA ACTCGGCGGA GAAATTGTCG AAGCCGCCGC 501 CATTTTGGAA TTTACCGACC TTCAAGGCGG CAAGAATATC CGTGCAAGCG 551 GCGCGCCCTT ATTTACCCTG CTTCAAAACG AAGGCTGTAT GAAGGGCTGA This corresponds to the amino acid sequence <SEQ ID 592; ORF 148>: ml48.pep 1 MALKTSΝLEH AMLVHPEAMS VGALADKIRK IEΝWPQKGIL FHDITPVLQS 51 AEYFRLLVDL LVYRYMDQKI DIVAGLDARG FIIGAALAYQ LΝVGFVPIRK 101 KGKLPFETVS QSYALEYGEA AVEIHTDAVK LGSRVLLVDD LIATGGTMLA 151 GLELIRKLGG EIVEAAAILE FTDLQGGKNI RASGAPLFTL LQNEGCMKG* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ml48 / gl48 99.0% identity in 199 aa overlap 10 20 30 40 50 60 ml48. pep MALKTSΝLEHAMLVHPEAMSVGALADKIRKIEΝWPQKGILFHDITPVLQSAEYFRLLVDL I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl48 MALKTSΝLEHAMLVHPEAMSVGALADKIRKIEΝWPQKGILFHDITPVLQSAEYFRLLVDL 10 20 30 40 50 60 70 80 90 100 110 120 ml48. pep LVYRYMDQKIDIVAGLDARGFIIGAALAYQLΝVGFVPIRKKGKLPFETVSQSYALEYGEA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl48 LVYRYMDQKIDIVAGLDARGFIIGAALAYQLΝVGFVPIRKKGKLPFETVSQSYALEYGEA 70 80 90 100 110 120 130 140 150 160 170 180 ml48. pep AVEIHTDAVKLGSRVLLVDDLIATGGTMLAGLELIRKLGGEIVEAAAILEFTDLQGGKΝI I I I I I I I I I I I I II I I I I I I: I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I gl48 AVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGGEIVEAAAILEFTDLQGGKΝI 130 140 150 160 170 180 190 200 ml48. pep RASGAPLFTLLQΝEGCMKGX I I I I I I I I I I I I I I I I I I I I gl48 RASGAPLFTLLQΝEGCMKGX 190 200 The following partial DΝA sequence was identified inN meningitidis <SEQ ID 593>: al48.seq 1 ATGGCGTTAA AAACATCAAA CTTGGAACAC GCAATGCTGG TTCATCCCGA 51 AGCTATGAGT GTCGGTGCGC TTGCCGACAA AATCCGCAAA ATCGAAAACT 101 GGCCGCAAAA AGGCATCTTA TTCCACGACA TCACGCCCGT CCTGCAAAGC 151 GCGGAATACT TCCGACTTTT GGTTGATTTA TTGGTTTACC GCTATATGGA 201 TCAGAAAATC GACATCGTTG CCGGTTTGGA CGCGCGCGGC TTCATTATCG 251 GCGCGGCACT CGCCTACCAG CTCAACGTCG GTTTCGTCCC CATCCGCAAA 301 AAAGGCAAGC TGCCTTTTGA AACCGTATCG CAAAGCTACG CGCTCGAATA 351 CGGGGAAGCT GCGGTGGAAA TCCACACCGA TGCCGTCAAA CTCGGTTCGC 401 GCGTGCTGCT GGTCGATGAT TTGGTTGCCA CGGGCGGCAC GATGCTTGCC 451 GGACTGGAGC TGATCCGCAA ACTCGGCGGG GAAATTGTCG AAGCCGCCGC 501 CATTTTGGAA TTTACCGACC TTCAAGGCGG CAAGAATATC CGTGCAAGCG 551 GCGCGCCCTT ATTTACCCTG CTTCAAAACG AAGGCTGTAT GAAGGGCTGA This corresponds to the amino acid sequence <SEQ ID 594; ORF 148.a>: al48.pep 1 MALKTSΝLEH AMLVHPEAMS VGALADKIRK IEΝWPQKGIL FHDITPVLQS 51 AEYFRLLVDL LVYRYMDQKI DIVAGLDARG FIIGAALAYQ LΝVGFVPIRK 101 KGKLPFETVS QSYALEYGEA AVEIHTDAVK LGSRVLLVDD LVATGGTMLA 151 GLELIRKLGG EIVEAAAILE FTDLQGGKΝI RASGAPLFTL LQΝEGCMKG* ml48/al48 99.5% identity in 199 aa overlap 10 20 30 40 50 60 ml48. pep MALKTSΝLEHAMLVHPEAMSVGALADKIRKIEΝWPQKGILFHDITPVLQSAEYFRLLVDL I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M al48 MALKTSΝLEHAMLVHPEAMSVGALADKIRKIEΝWPQKGILFHDITPVLQSAEYFRLLVDL 10 20 30 40 50 60 70 80 90 100 110 120 ml 48. pep LVYRYMDQKIDIVAGLDARGFIIGAALAYQLNVGFVPIRKKGKLPFETVSQSYALEYGEA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al48 LVYRYMDQKIDIVAGLDARGFIIGAALAYQLNVGFVPIRKKGKLPFETVSQSYALEYGEA 70 80 90 100 110 120 130 140 150 160 170 180 ml 8. pep AVEIHTDAVKLGSRVLLVDDLIATGGTMLAGLELIRKLGGEIVEAAAILEFTDLQGGKNI I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I al48 AVEIHTDAVKLGSRVLLVDDLVATGGTMLAGLELIRKLGGEIVEAAAILEFTDLQGGKNI 130 140 150 160 170 180 190 200 ml48.pep RASGAPLFTLLQNEGCMKGX I I I I I I I I I I I I I I I I I I I I al48 RASGAPLFTLLQNEGCMKGX 190 200 The following partial DNA sequence was identified in N.gonorrhoeae <SEQ ID 595>: gl49.seq 1 ATGTTGATTG ACAACAATGT CCGCCATTAC AGCTTTTTCG GTGTAGAACA 51 GGCAAATTGG GACAACTTCA CGCTTGAAGG CGGCGTACGC GTGGAAAAAC 101 AAAAAGCCTC CATCCGGTAC GACAAAGCAT TGATTGATCG AGAAAACTAC 151 TACAACCAGC CCCTGCCCGA CCTCGGCGCG CACCGCCAAA CCGCCCGCTC 201 GTTCGCACTT TCGGGCAACT GGTATTTCAC GCCACACCAC AAACTCAGCC 251 TGACCGCCTC CCATCAGGAa cgCCTGCCGT CAACGCaagA actGtACgca 301 cacggcAAGC ACGtcgccac CAACACCTTT GAagtcggca acaaACACCT 351 CAACAAAGaG CgttccaacA atatcgaACT CGCGCTGGgc tAcaaaggcg 401 accGCTGGCA ATACAATCTG GCAGCCTACC GCAACCGAtT CGGCAACTAC 451 ATTTACGCCC AAACCTTAaa cgacggacgC GGCCCCAAAT CCATCgaaga 501 cgacagcgaA ATGaagcTCG TGCGCTACAA CCAATCCGGT GCCGACTTCT 551 ACGgcgcggA aggcgaaatc tACTTcaaaC CGAcACCGCG CTACCGCATC 601 GGTGTTTCCG GCGACTatgt acgaggccgT CTGAAAAACC TGCCGTCCCT 651 ACCCGGCAGG gaagatccCT AcggcAAACG TCccttcaTC GCACAAGCCG 701 ACCAAAACGC CCCCCGCATT ccggctGCGC GCCTCGGCTT CCACCTGAAA 751 ACCTCGCTAA CCGACCGTAT CGATGCCAAT TTGGACTACT ACCGCGTGTT 801 CGCCCAAAAC AAACTCGCCC GCTACGAAAC GCGTACGCCC GGACACCATA 851 TGCTCAACCT CGGTGCAAAC TACCGCCGCA ATACGCGCTA TGGCGAGTGG 901 AATTGGTACG TCAAAGCCGA CAACCTGCtc aACcaatCcg tTTACGCCCa 951 cAGCAGCTTC CTCTCTGATA CGCCGCAAAt gGGCCGCAGC TTtgccgGCg 1001 gcgtaAACGT GaAGTTttaA This corresponds to the amino acid sequence <SEQ ID 596; ORF 149.ng>: gl49.pep 1 MLIDNNVRHY SFFGVEQANW DNFTLEGGVR VEKQKASIRY DKALIDRENY 51 YNQPLPDLGA HRQTARSFAL SGNWYFTPHH KLSLTASHQE RLPSTQELYA 101 HGKHVATNTF EVGNKHLNKE RSNNIELALG YKGDRWQYNL AAYRNRFGNY 151 IYAQTLNDGR GPKSIEDDSE MKLVRYNQSG ADFYGAEGEI YFKPTPRYRI 201 GVSGDYVRGR LKNLPSLPGR EDPYGKRPFI AQADQNAPRI PAARLGFHLK 251 TSLTDRIDAN LDYYRVFAQN KLARYETRTP GHHMLNLGAN YRRNTRYGEW 301 NWYVKADNLL NQSVYAHSSF LSDTPQMGRS FAGGVNVKF* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 597>: ml49. seq 1 ATGCTGCTTG ACAACAAAGT GCAACATTAC AGCTTTTTCG GTGTAGAACA 51 GGCAAACTGG GACAACTTCA CGCTTGAAGG AGGCGTACGC GTGGAAAAAC 101 AAAAAGCCTC CATTCAGTAC GACAAAGCAT TGATTGATCG GGAAAACTAC 151 TACAACCACC CCCTGCCCGA CCTCGGCGCG CACCGCCAAA CCGCCCGCTC 201 ATTCGCACTT TCGGGCAACT GGTATTTCAC GCCACAACAC AAACTCAGCC 251 TGACCGCCTC CCATCAGGAA CGCCTGCCGT CAACGCAAGA GCTGTACGCA 301 CACGGCAAAC ACGTCGCCAC CAACACCTTT GAAGTCGGCA ACAAACACCT 351 CAACAAAGAG CGTTCCAACA ATATCGAACT CGCGCTGGGC TACGAAGGCG 401 ACCGCTGGCA ATACAATCTG GCACTCTACC GCAACCGCTT CGGTAACTAC 451 ATTTACGCCC AAACCTTAAA CGACGGACGC GGCCCCAAAT CCATCGAAGA 501 CGACAGCGAA ATGAAGCTCG TGCGCTACAA CCAATCCGGC GCCGACTTCT 551 ACGGCGCGGA AGGCGAAATC TACTTCAAAC CGACACCGCG CTACCGCATC 601 GGCGTTTCCG GCGACTATGT ACGAGGCCGT CTGAAAAACC TGCCTTCCCT 651 ACCCGGCAGA GAAGATGCCT ACGGCAACCG TCCTTTCATC GCACAGGACG 701 ACCAAAATGC CCCCCGTGTT CCGGCTGCGC GCCTCGGCTT CCACCTGAAA 751 GCCTCGCTGA CCGACCGTAT CGATGCCAAT TTGGACTACT ACCGCGTGTT 801 CGCCCAAAAC AAACTCGCCC GCTACGAAAC GCGCACGCCC GGACACCATA 851 TGCTCAACCT CGGCGCAAAC TACCGCCGCA ATACGCGCTA TGGCGAGTGG 901 AATTGGTACG TCAAAGCCGA CAACCTGCTC AACCAATCCG TTTACGCCCA 951 CAGCAGCTTT CTCTCTGATA CGCCGCAAAT GGGCCGCAGC TTTACCGGCG 1001 GCGTGAACGT GAAGTTTTAA This corresponds to the amino acid sequence <SEQ ID 598; ORF 149>: ml49.pep 1 MLLDNKVQHY SFFGVEQANW DNFTLEGGVR VEKQKASIQY DKALIDRENY 51 YNHPLPDLGA HRQTARSFAL SGNWYFTPQH KLSLTASHQE RLPSTQELYA 101 HGKHVATNTF EVGNKHLNKE RSNNIELALG YEGDRWQYNL ALYRNRFGNY 151 IYAQTLNDGR GPKSIEDDSE MKLVRYNQSG ADFYGAEGEI YFKPTPRYRI 201 GVSGDYVRGR LKNLPSLPGR EDAYGNRPFI AQDDQNAPRV PAARLGFHLK 251 ASLTDRIDAN LDYYRVFAQN KLARYETRTP GHHMLNLGAN YRRNTRYGEW 301 NWYVKADNLL NQSVYAHSSF LSDTPQMGRS FTGGVNVKF* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 149 shows 95.9%) identity over a 339 aa overlap with a predicted ORF (ORF 149.ng) from N gonorrhoeae: ml49/gl49 10 20 30 40 50 60 ml49. pep MLLDΝKVQHYSFFGVEQAΝWDΝFTLEGGVRVEKQKASIQYDKALIDREΝYYΝHPLPDLGA gl49 MLIDΝΝVRHYSFFGVEQAΝWDΝFTLEGGVRVEKQKASIRYDKALIDREΝYYΝQPLPDLGA 10 20 30 40 50 60 70 80 90 100 110 120 ml49. pep HRQTARSFALSGΝWYFTPQHKLSLTASHQERLPSTQELYAHGKHVATΝTFEVGΝKHLΝKE gl49 HRQTARSFALSGΝWYFTPHHKLSLTASHQERLPSTQELYAHGKHVATΝTFEVGΝKHLΝKE 70 80 90 100 110 120 130 140 150 160 170 180 ml49.pep RSΝΝIELALGYEGDRWQYΝLALYRΝRFGΝYIYAQTLΝDGRGPKSIEDDSEMKLVRYΝQSG I II II 11111 hi 111 M I II MMMMMMMMMMMMMMMIMIMM gl49 RSΝΝIELALGYKGDRWQYΝLAAYRΝRFGΝYIYAQTLΝDGRGPKSIEDDSEMKLVRYΝQSG 130 140 150 160 170 180 190 200 210 220 230 240 ml49.pep ADFYGAEGEIYFKPTPRYRIGVSGDYVRGRLKΝLPSLPGREDAYGΝRPFIAQDDQΝAPRV MMMMMMMMMMMMMMMMMMMMM IhllMM lllllh gl49 ADFYGAEGEIYFKPTPRYRIGVSGDYVRGRLKΝLPSLPGREDPYGKRPFIAQADQΝAPRI 190 200 210 220 230 240 250 260 270 280 290 300 ml49.pep PAARLGFHLKASLTDRIDAΝLDYYRVFAQΝKLARYETRTPGHHMLΝLGAΝYRRΝTRYGEW MMMMIhlllll II III Mill II MM I Mill III Mill gl49 PAARLGFHLKTSLTDRIDAΝLDYYRVFAQΝKLARYETRTPGHHMLΝLGAΝYRRΝTRYGEW 250 260 270 280 290 300 310 320 330 340 ml49.pep ΝWYVKADΝLLΝQSVYAHSSFLSDTPQMGRSFTGGVΝVKFX g149 NWYVKADNLLNQSVYAHSSFLSDTPQMGRSFAGGVNVKFX 310 320 330 340 The following partial DNA sequence was identified in N meningitidis <SEQ TD 599>: al49. seq 1 ATGCTGCTTG ACAATAAAGT GCAACATTAC AGCTTTTTCG GTGTAGAACA 51 GGCAAACTGG GACAACTTCA CGCTTGAAGG CGGCGTACGC GTGGAAAAAC 101 AAAAAGCCTC CATCCGCTAC GACAAAGCAT TGATTGATCG GGAAAACTAC 151 TACAACCATC CCCTGCCCGA CCTCGGCGCG CACCGCCAAA CCGCCCGCTC 201 ATTCGCACTT TCGGGCAACT GGTATTTCAC GCCACAACAC AAACTCAGCC 251 TGACCGCCTC CCATCAGGAA CGCCTGCCGT CAACGCAAGA GCTGTACGCA 301 CACGGCAAAC ACGTCGCCAC CAACACCTTT GAAGTCGGCA ACAAACACCT 351 CAACAAAGAG CGTTCCAACA ATATCGAACT CGCGCTGGGC TACGAAGGCG 401 ACCGCTGGCA ATACAATCTG GCACTCTACC GCAACCGCTT CGGCAACTAC 451 ATTTACGCCC AAACCTTAAA CGACGGACGC GGCCCCAAAT CCATCGAAGA 501 CGACAGCGAA ATGAAGCTCG TGCGCTACAA CCAATCCGGT GCGGACTTCT 551 ACGGCGCGGA AGGCGAAATC TACTTCAAAC CGACACCGCG CTACCGCATC 601 GGCGTTTCCG GCGACTATGT ACGAGGCCGT CTGAAAAACC TGCCTTCCCT 651 ACCCGGCAGG GAAGACGCCT ACGGCAACCG CCCACTCATT GCCCAAGCCG 701 ACCAAAACGC CCCTCGCGTT CCGGCTGCGC GCCTCGGCGT CCACCTGAAA 751 GCCTCGCTGA CCGACCGCAT CGATGCCAAT TTGGACTACT ACCGCGTGTT 801 CGCCCAAAAC AAACTCGCCC GCTACGAAAC GCGCACGCCC GGACACCATA 851 TGCTCAACCT CGGCGCAAAC TACCGCCGCA ATACGCGCTA TGGCGAGTGG 901 AATTGGTACG TCAAAGCCGA CAACCTGCTC AACCAATCCG TTTACGCCCA 951 CAGCAGCTTC CTCTCTGATA CGCCGCAAAT GGGCCGCAGC TTTACCGGCG 1001 GCGTGAACGT GAAGTTTTAA This corresponds to the amino acid sequence <SEQ ID 600; ORF 149.a>: al49.pep 1 MLLDΝKVQHY SFFGVEQAΝW DΝFTLEGGVR VEKQKASIRY DKALIDREΝY 51 YΝHPLPDLGA HRQTARSFAL SGΝWYFTPQH KLSLTASHQE RLPSTQELYA 101 HGKHVATΝTF EVGΝKHLΝKE RSΝΝIELALG YEGDRWQYΝL ALYRΝRFGΝY 151 IYAQTLΝDGR GPKSIEDDSE MKLVRYΝQSG ADFYGAEGEI YFKPTPRYRI 201 GVSGDYVRGR LKΝLPSLPGR EDAYGΝRPLI AQADQΝAPRV PAARLGVHLK 251 ASLTDRIDAΝ LDYYRVFAQΝ KLARYETRTP GHHMLΝLGAΝ YRRΝTRYGEW 301 ΝWYVKADΝLL ΝQSVYAHSSF LSDTPQMGRS FTGGVΝVKF* ml49/al49 98.8% identity in 339 aa overlap 10 20 30 40 50 60 ml49. pep MLLDΝKVQHYSFFGVEQAΝWDΝFTLEGGVRVEKQKASIQYDKALIDREΝYYΝHPLPDLGA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I: I I I I I I I I I I I I I I I I I I I I I al 9 MLLDΝKVQHYSFFGVEQAΝWDΝFTLEGGVRVEKQKASIRYDKALIDREΝYYΝHPLPDLGA 10 20 30 40 50 60 70 80 90 100 110 120 ml49. pep HRQTARSFALSGΝWYFTPQHKLSLTASHQERLPSTQELYAHGKHVATΝTFEVGΝKHLΝKE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I al49 HRQTARSFALSGΝWYFTPQHKLSLTASHQERLPSTQELYAHGKHVATΝTFEVGΝKHLΝKE 70 80 90 100 110 120 130 140 150 160 170 180 ml49. pep RSΝΝIELALGYEGDRWQYΝLALYRΝRFGΝYIYAQTLΝDGRGPKSIEDDSEMKLVRYΝQSG I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al 9 RSΝΝIELALGYEGDRWQYΝLALYRΝRFGΝYIYAQTLΝDGRGPKSIEDDSEMKLVRYΝQSG 130 140 150 160 170 180 190 200 210 220 230 240 ml49. pep ADFYGAEGEIYFKPTPRYRIGVSGDYVRGRLKΝLPSLPGREDAYGΝRPFIAQDDQΝAPRV I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I lllllll al49 ADFYGAEGEIYFKPTPRYRIGVSGDYVRGRLKΝLPSLPGREDAYGΝRPLIAQADQΝAPRV 190 200 210 220 230 240 250 260 270 280 290 300 ml49. pep PAARLGFHLKASLTDRIDAΝLDYYRVFAQΝKLARYETRTPGHHMLΝLGAΝYRRΝTRYGEW llllll I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al49 PAARLGVHLKASLTDRIDANLDYYRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEW 250 260 270 280 290 300 310 320 330 340 ml 49. pep NWYVKADNLLNQSVYAHSSFLSDTPQMGRSFTGGVNVKFX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I a149 NWYVKADNLLNQSVYAHSSFLSDTPQMGRSFTGGVNVKFX 310 320 330 340 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 601>: gl49-l.seq 1 ATGGCACAAA TCACACTCAA ACCCATTGTT TTATCAATTC TTTTAATCAA 51 CACACCCCTC CTCGCCCAAG CGCATGAAAC TGAGCAATCG GTGGGCTTGG 101 AAACGGTCAG CGTCGTCGGC AAAAGCCGTC CGCGCGCGAC TTCGGGGCTG 151 CTGCACACTT CGACCGCCTC CGACAAAATC ATCTCCGGCG ATACTTTGCG 201 CCAAAAAGCC GTCAACTTGG GCGACGCTTT GGACGGCGTA CCGGGCATCC 251 ACGCTTCGCA ATACGGCGGC GGCGCATCCG CTCCCGTTAT TCGCGGTCAA 301 ACGGGCAGAC GGATTAAAGT ATTGAACCAT CACGGCGAAA CGGGCGATAT 351 GGCGGACTTT TCTCCCGATC ACGCCATTAT GGTAGATACC GCCTTGTCGC 401 AACAGGTTGA AATCCTGCGC GGGCCGGTTA CGCTCTTGTA CAGCTCGGGC 451 AATGTGGCGG GGCTGGTCGA TGTTGCCGAT GGAAAAATCC CCGAAAAAAT 501 GCCTGAAAAC GGCGTATCGG GCGaagccgG ATTGCGTTTG AGCAGCGGCA 551 ATTTAGAAAA ACTGACATCC GCAGGCATCA ATATCGGACT GGGCAAAAAC 601 TTCGTGCTGC ATACCGAAGG CTTGTACCGC AAATCGGGCG ATTACGCCGT 651 ACCGCGTTAC CGCAATCTGA AACGCCTGCC CGACAGCCAT GCCGATTCGC 701 AAACGGGCAG CATCGGGCTG TCTTGGGTGG GCGAAAAAGG CTTTATCGGC 751 GCAGCATACA GCGACCGTCG CGACCGCTAC GGCCTGCCTG CCCACAGCCA 801 CGAATACGAT GATTGCCACG CCGACATCAT CTGGCAAAAG AGTTTGATCA 851 ACAAACGCTA TTTGCAGCTT TATCCGCACT TGTTGACCGA AGAAGACATC 901 GATTACGACA ATCCGGGCTT GAGCTGCGGC TTCCACGACG GCGACGGTGC 951 ACACGCACAC ACCCACAACG GCAAACCGTG GATAGACCTG CGCAACAAAC 1001 GCTACGAACT CCGCGCCGAA TGGAAGCAGC CATTCCCCGG TTTTGAAGCC 1051 CTGCGCGTAC ATCTGAACCG CAATGACTAC CACCACGACG AAAAAGCAGG 1101 CGATGCAGTA GAAAACTTCT TCAACAACAA AACACACAAC GCCCGTATCG 1151 AGTTGCGCCA CCAACCCATA GGCCGTCTGA AAGGCAGCTG GGGCGTGCAA 1201 TATTTGGGAC AAAAATCCAG CGCGCTTTCC GCCATTCCCG AAACCGTCCA 1251 ACAACCGATG TTGATTGACA ACAATGTCCG CCATTACAGC TTTTTCGGTG 1301 TAGAACAGGC AAATTGGGAC AACTTCACGC TTGAAGGCGG CGTACGCGTG 1351 GAAAAACAAA AAGCCTCCAT CCGGTACGAC AAAGCATTGA TTGATCGAGA 1401 AAACTACTAC AACCAGCCCC TGCCCGACCT CGGCGCGCAC CGCCAAACCG 1451 CCCGCTCGTT CGCACTTTCG GGCAACTGGT ATTTCACGCC ACACCACAAA 1501 CTCAGCCTGA CCGCCTCCCA TCAGGAACGC CTGCCGTCAA CGCAAGAACT 1551 GTACGCACAC GGCAAGCACG TCGCCACCAA CACCTTTGAA GTCGGCAACA 1601 AACACCTCAA CAAAGAGCGT TCCAACAATA TCGAACTCGC GCTGGGCTAC 1651 GAAGGCGACC GCTGGCAATA CAATCTGGCA GCCTACCGCA ACCGATTCGG 1701 CAACTACATT TACGCCCAAA CCTTAAACGA CGGACGCGGC CCCAAATCCA 1751 TCGAAGACGA CAGCGAAATG AAGCTCGTGC GCTACAACCA ATCCGGTGCC 1801 GACTTCTACG GCGCGGAAGG CGAAATCTAC TTCAAACCGA CACCGCGCTA 1851 CCGCATCGGT GTTTCCGGCG ACTATGTACG AGGCCGTCTG AAAAACCTGC 1901 CGTCCCTACC CGGCAGGGAA GATCCCTACG GCAAACGTCC CTTCATCGCA 1951 CAAGCCGACC AAAACGCCCC CCGCATTCCG GCTGCGCGCC TCGGCTTCCA 2001 CCTGAAAACC TCGCTAACCG ACCGTATCGA TGCCAATTTG GACTACTACC 2051 GCGTGTTCGC CCAAAACAAA CTCGCCCGCT ACGAAACGCG TACGCCCGGA 2101 CACCATATGC TCAACCTCGG TGCAAACTAC CGCCGCAATA CGCGCTATGG 2151 CGAGTGGAAT TGGTACGTCA AAGCCGACAA CCTGCTCAAC CAATCCGTTT 2201 ACGCCCACAG CAGCTTCCTC TCTGATACGC CGCAAATGGG CCGCAGCTTT 2251 ACCGGCGGCG TAAACGTGAA GTTTTAA This corresponds to the amino acid sequence <SEQ ID 602; ORF 149-l.ng>: gl49-l.pep 1 MAQITLKPIV LSILLIΝTPL LAQAHETEQΞ VGLETVSWG KSRPRATSGL 51 LHTΞTASDKI ISGDTLRQKA VΝLGDALDGV PGIHAΞQYGG GASAPVIRGQ 101 TGRRIKVLΝH HGETGDMADF SPDHAIMVDT ALSQQVEILR GPVTLLYSSG 151 ΝVAGLVDVAD GKIPEKMPEΝ GVSGEAGLRL SSGΝLEKLTΞ AGIΝIGLGKΝ 201 FVLHTEGLYR KSGDYAVPRY RΝLKRLPDSH ADSQTGSIGL SWVGEKGFIG 251 AAYSDRRDRY GLPAHSHEYD DCHADIIWQK SLIΝKRYLQL YPHLLTEEDI 301 DYDΝPGLSCG FHDGDGAHAH THΝGKPWIDL RΝKRYELRAE WKQPFPGFEA 351 LRVHLNRNDY HHDEKAGDAV ENFFNNKTHN ARIELRHQPI GRLKGΞWGVQ 401 YLGQKSSALS AIPETVQQPM LIDNNVRHYS FFGVEQANWD NFTLEGGVRV 451 EKQKASIRYD KALIDRENYY NQPLPDLGAH RQTARSFALS GNWYFTPHHK 501 LSLTASHQER LPSTQELYAH GKHVATNTFE VGNKHLNKER SNNIELALGY 551 EGDRWQYNLA AYRNRFGNYI YAQTLNDGRG PKSIEDDSEM KLVRYNQSGA 601 DFYGAEGEIY FKPTPRYRIG VSGDYVRGRL KNLPSLPGRE DPYGKRPFIA 651 QADQNAPRIP AARLGFHLKT SLTDRIDANL DYYRVFAQNK LARYETRTPG 701 HHMLNLGANY RRNTRYGEWN WYVKADNLLN QSVYAHSSFL SDTPQMGRSF 751 TGGVNVKF* The following partial DNA sequence was identified in N meningitidis <SEQ ID 603>: ml49-l.seq 1 ATGGCACAAA CTACACTCAA ACCCATTGTT TTATCAATTC TTTTAATCAA 51 CACACCCCTC CTCGCCCAAG CGCATGAAAC TGAGCAATCG GTGGATTTGG 101 AAACGGTCAG CGTCGTCGGC AAAAGCCGTC CGCGCGCCAC GTCGGGGCTG 151 TTGCACACTT CGACCGCCTC CGACAAAATC ATCTCCGGCG ATACCTTGCG 201 CCAAAAAGCC GTCAACTTGG GCGACGCTTT AGACGGCGTA CCGGGCATCC 251 ACGCTTCGCA ATACGGCGGC GGCGCGTCTG CTCCCGTCAT TCGCGGTCAA 301 ACAGGCAGGC GGATTAAAGT GTTGAACCAT CACGGCGAAA CAGGCGATAT 351 GGCGGATTTT TCGCCCGATC ACGCCATTAT GGTAGATACC GCCTTGTCGC 401 AACAGGTCGA AATCCTGCGC GGGCCGGTTA CGCTCTTGTA CAGCTCGGGC 451 AATGTGGCGG GGCTGGTCGA TGTTGCCGAT GGCAAAATCC CCGAAAAAAT 501 GCCTGAAAAC GGCGTATCGG GCGAACTCGG ATTGCGTTTG AGCAGCGGCA 551 ATCTGGAAAA ACTCACGTCC GGCGGCATCA ATATCGGTTT GGGCAAAAAC 601 TTTGTATTGC ACACGGAAGG GCTGTACCGC AAATCGGGGG ATTACGCCGT 651 ACCGCGTTAC CGCAATCTGA AACGCCTGCC CGACAGCCAC GCCGATTCGC 701 AAACGGGCAG CATCGGGCTG TCTTGGGTTG GCGAAAAAGG TTTTATCGGC 751 GTAGCGTACA GCGACCGTCG CGACCAATAT GGTCTGCCTG CCCACAGCCA 801 CGAATACGAT GATTGCCACG CCGACATCAT CTGGCAAAAG AGCTTGATTA 851 ACAAACGCTA TTTACAGCTT TATCCGCACC TGTTGACCGA AGAAGACATC 901 GATTACGACA ATCCGGGCTT GAGCTGCGGC TTCCACGACG ACGATAATGC 951 ACACGCACAC ACCCACAGCG GCAGACCGTG GATAGACCTG CGCAACAAAC 1001 GCTACGAACT CCGTGCCGAA TGGAAGCAAC CGTTCCCCGG TTTTGAAGCC 1051 CTGCGCGTAC ACCTGAACCG CAACGACTAC CGCCACGACG AAAAAGCAGG 1101 CGATGCAGTC GAAAACTTTT TTAACAACCA AACGCAAAAC GCCCGCATCG 1151 AGTTGCGCCA CCAACCCATA GGTCGTCTGA AAGGCAGCTG GGGCGTGCAA 1201 TATTTACAAC AAAAATCCAG TGCTTTATCT GCCATATCCG AAGCGGTTAA 1251 ACAACCGATG CTGCTTGACA ACAAAGTGCA ACATTACAGC TTTTTCGGTG 1301 TAGAACAGGC AAACTGGGAC AACTTCACGC TTGAAGGAGG CGTACGCGTG 1351 GAAAAACAAA AAGCCTCCAT TCAGTACGAC AAAGCATTGA TTGATCGGGA 1401 AAACTACTAC AACCACCCCC TGCCCGACCT CGGCGCGCAC CGCCAAACCG 1451 CCCGCTCATT CGCACTTTCG GGCAACTGGT ATTTCACGCC ACAACACAAA 1501 CTCAGCCTGA CCGCCTCCCA TCAGGAACGC CTGCCGTCAA CGCAAGAGCT 1551 GTACGCACAC GGCAAACACG TCGCCACCAA CACCTTTGAA GTCGGCAACA 1601 AACACCTCAA CAAAGAGCGT TCCAACAATA TCGAACTCGC GCTGGGCTAC 1651 GAAGGCGACC GCTGGCAATA CAATCTGGCA CTCTACCGCA ACCGCTTCGG 1701 TAACTACATT TACGCCCAAA CCTTAAACGA CGGACGCGGC CCCAAATCCA 1751 TCGAAGACGA CAGCGAAATG AAGCTCGTGC GCTACAACCA ATCCGGCGCC 1801 GACTTCTACG GCGCGGAAGG CGAAATCTAC TTCAAACCGA CACCGCGCTA 1851 CCGCATCGGC GTTTCCGGCG ACTATGTACG AGGCCGTCTG AAAAACCTGC 1901 CTTCCCTACC CGGCAGAGAA GATGCCTACG GCAACCGTCC TTTCATCGCA 1951 CAGGACGACC AAAATGCCCC CCGTGTTCCG GCTGCGCGCC TCGGCTTCCA 2001 CCTGAAAGCC TCGCTGACCG ACCGTATCGA TGCCAATTTG GACTACTACC 2051 GCGTGTTCGC CCAAAACAAA CTCGCCCGCT ACGAAACGCG CACGCCCGGA 2101 CACCATATGC TCAACCTCGG CGCAAACTAC CGCCGCAATA CGCGCTATGG 2151 CGAGTGGAAT TGGTACGTCA AAGCCGACAA CCTGCTCAAC CAATCCGTTT 2201 ACGCCCACAG CAGCTTTCTC TCTGATACGC CGCAAATGGG CCGCAGCTTT 2251 ACCGGCGGCG TGAACGTGAA GTTTTAA This corresponds to the amino acid sequence <SEQ ID 604; ORF 149-1>: ml49-l.pep 1 MAQTTLKPIV LSILLIΝTPL LAQAHETEQS VDLETVSVVG KSRPRATSGL 51 LHTSTASDKI ISGDTLRQKA VΝLGDALDGV PGIHAΞQYGG GASAPVIRGQ 101 TGRRIKVLΝH HGETGDMADF SPDHAIMVDT ALSQQVEILR GPVTLLYSSG 151 ΝVAGLVDVAD GKIPEKMPEΝ GVSGELGLRL ΞSGΝLEKLTS GGIΝIGLGKΝ 201 FVLHTEGLYR KSGDYAVPRY RΝLKRLPDSH ADSQTGSIGL SWVGEKGFIG 251 VAYSDRRDQY GLPAHSHEYD DCHADIIWQK SLIΝKRYLQL YPHLLTEEDI 301 DYDΝPGLSCG FHDDDΝAHAH THSGRPWIDL RΝKRYELRAE WKQPFPGFEA 351 LRVHLΝRΝDY RHDEKAGDAV EΝFFΝΝQTQΝ ARIELRHQPI GRLKGSWGVQ 401 YLQQKSSALS AISEAVKQPM LLDΝKVQHYS FFGVEQANWD NFTLEGGVRV 451 EKQKASIQYD KALIDRENYY NHPLPDLGAH RQTARSFALS GNWYFTPQHK 501 LSLTASHQER LPSTQELYAH GKHVATNTFE VGNKHLNKER SNNIELALGY 551 EGDRWQYNLA LYR RFGNYI YAQTLNDGRG PKΞIEDDSEM KLVRYNQΞGA 601 DFYGAEGEIY FKPTPRYRIG VSGDYVRGRL KNLPSLPGRE DAYGNRPFIA 651 QDDQNAPRVP AARLGFHLKA ΞLTDRIDANL DYYRVFAQNK LARYETRTPG 701 HHMLNLGANY RRNTRYGEWN WYVKADNLLN QΞVYAHSSFL SDTPQMGRSF 751 TGGVNVKF* ml49-l/gl49-l 96.2% identity in 758 aa overlap 10 20 30 40 50 60 ml49-l.pep MAQTTLKPIVLΞILLINTPLLAQAHETEQSVDLETVSWGKSRPRATSGLLHTSTASDKI III I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl49-l MAQITLKPIVLSILLINTPLLAQAHETEQSVGLETVSWGKSRPRATΞGLLHTSTASDKI 10 20 30 40 50 60 70 80 90 100 110 120 ml49-l.pep ISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTGRRIKVLNHHGETGDMADF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I gl49-l ISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTGRRIKVLNHHGETGDMADF 70 80 90 100 110 120 130 140 150 160 170 180 ml49-l.pep SPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVADGKIPEKMPENGVSGELGLRL I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl49-l SPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVADGKIPEKMPENGVΞGEAGLRL 130 140 150 160 170 180 190 200 210 220 230 240 ral49-l.pep SSGNLEKLTSGGINIGLGKNFVLHTEGLYRKSGDYAVPRYRNLKRLPDSHADSQTGΞIGL I I I I I I I I I I :l I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl49-l SSGNLEKLTΞAGINIGLGKNFVLHTEGLYRKSGDYAVPRYRNLKRLPDSHADSQTGSIGL 190 200 210 220 230 240 250 260 270 280 290 300 ml49-l.pep SWVGEKGFIGVAYSDRRDQYGLPAHSHEYDDCHADIIWQKSLINKRYLQLYPHLLTEEDI I I I I I I I I I I :| I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl49-l SWVGEKGFIGAAYSDRRDRYGLPAHSHEYDDCHADIIWQKSLINKRYLQLYPHLLTEEDI 250 260 270 280 290 300 310 320 330 340 350 360 ml49-l.pep DYDNPGLSCGFHDDDNAHAHTHSGRPWIDLRNKRYELRAEWKQPFPGFEALRVHLNRNDY I I I I I I I I I I I I I I : I I I I I I : I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl49-l DYDNPGLSCGFHDGDGAHAHTHNGKPWIDLRNKRYELRAEWKQPFPGFEALRVHLNRNDY 310 320 330 340 350 360 370 380 390 400 410 420 ml49-l.pep RHDEKAGDAVENFFNNQTQNARIELRHQPIGRLKGSWGVQYLQQKSΞALSAISEAVKQPM : I I I I I I I I I I I I I I I :| :| I I I I I I I I I I I I I I I I I I I I I I lllllllll I :| :| I I gl49-l HHDEKAGDAVENFFNNKTHNARIELRHQPIGRLKGSWGVQYLGQKSSALΞAIPETVQQPM 370 380 390 400 410 420 430 440 450 460 470 480 ml49-1.pep LLDNKVQHYSFFGVEQANWDNFTLEGGVRVEKQKAΞIQYDKALIDRENYYNHPLPDLGAH I :| I : I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I :l I I I I I I I I I I I I :| I I I I I I I gl49-l LIDNNVRHYSFFGVEQANWDNFTLEGGVRVEKQKASIRYDKALIDRENYYNQPLPDLGAH 430 440 450 460 470 480 490 500 510 520 530 540 ml49-1. pep RQTARSFALΞGNWYFTPQHKLSLTASHQERLPΞTQELYAHGKHVATNTFEVGNKHLNKER I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl49-l RQTARSFALΞGNWYFTPHHKLΞLTASHQERLPSTQELYAHGKHVATNTFEVGNKHLNKER 490 500 510 520 530 540 550 560 570 580 590 600 ml49-l.pep SNNIELALGYEGDRWQYNLALYRNRFGNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQΞGA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl49-l SNNIELALGYEGDRWQYNLAAYRNRFGNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGA 550 560 570 580 590 600 610 620 630 640 650 660 ml49-l.pep DFYGAEGEIYFKPTPRYRIGVSGDYVRGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVP I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I 11:111111 I I I I I |:| gl49-l DFYGAEGEIYFKPTPRYRIGVSGDYVRGRLKNLPSLPGREDPYGKRPFIAQADQNAPRIP 610 620 630 640 650 660 670 680 690 700 710 720 ml49-l.pep AARLGFHLKASLTDRIDANLDYYRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWN I I I I I I I I I :| I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl49-l AARLGFHLKTSLTDRIDANLDYYRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWN 670 680 690 700 710 720 730 740 750 759 ml49-l .pep WYVKADNLLNQSVYAHSΞFLSDTPQMGRSFTGGVNVKFX I I I I I I I I I I I I I I I I I I I I I I II II I I I I I I I I II I I I gl49-l WYVKADNLLNQSVYAHSSFLSDTPQMGRSFTGGVNVKFX 730 740 750 The following partial DNA sequence was identified in N meningitidis <SEQ LD 605>: al49-l.seq 1 ATGGCACAAA CTACACTCAA ACCCATTGTT TTATCAATTC TTTTAATCAA 51 CACACCCCTC CTCTCCCAAG CGCATGGAAC TGAGCAATCA GTGGGCTTGG 101 AAACGGTCAG CGTCGTCGGC AAAAGCCGTC CGCGCGCCAC TTCGGGGCTG 151 CTGCACACTT CTACCGCCTC CGACAAAATC ATCAGCGGCG ACACCTTGCG 201 ACAAAAAGCC GTCAACTTGG GTGATGCTTT AGACGGCGTA CCGGGCATTC 251 ATGCCTCGCA ATACGGCGGC GGCGCATCCG CTCCCGTTAT TCGCGGTCAA 301 ACAGGCAGAC GGATTAAAGT GTTGAACCAT CACGGCGAAA CGGGCGACAT 351 GGCGGACTTC TCTCCAGACC ATGCAATCAT GGTGGACAGC GCCTTGTCGC 401 AACAGGTCGA AATCCTGCGC GGTCCGGTTA CGCTCTTGTA CAGCTCGGGC 451 AATGTGGCGG GGCTGGTCGA TGTTGCCGAT GGCAAAATCC CCGAAAAAAT 501 GCCTGAAAAC GGCGTATCGG GCGAACTCGG ATTGCGTTTG AGCAGCGGCA 551 ATCTGGAAAA ACTCACGTCC GGCGGCATCA ATATCGGTTT GGGCAAAAAC 601 TTTGTATTGC ACACGGAAGG GCTGTACCGC AAATCGGGGG ATTACGCCGT 651 ACCGCGTTAC CGCAATCTGA AACGCCTGCC CGACAGCCAC GCCGATTCGC 701 AAACGGGCAG CATCGGGCTG TCTTGGGTTG GCGAAAAAGG CTTTATCGGC 751 GCAGCATACA GCGACCGTCG CGACCAATAT GGTCTGCCTG CCCACAGCCA 801 CGAATACGAT GATTGCCACG CCGACATCAT CTGGCAAAAG AGTTTGATTA 851 ACAAACGCTA TTTGCAGCTT TATCCGCACC TGTTGACCGA AGAAGACATC 901 GATTACGACA ATCCGGGCTT GAGCTGCGGC TTTCACGACG ACGATGATGC 951 ACACGCCCAT GCCCACAACG GCAAACCTTG GATAGACCTG CGCAACAAAC 1001 GCTACGAACT CCGCGCCGAA TGGAAGCAAC CGTTCCCCGG TTTTGAAGCC 1051 CTGCGCGTAC ACCTGAACCG CAACGACTAC CGCCACGACG AAAAAGCAGG 1101 CGATGCAGTA GAAAACTTTT TTAACAACCA AACGCAAAAC GCCCGTATCG 1151 AGTTGCGCCA CCAACCCATA GGCCGTCTGA AAGGCAGCTG GGGCGTGCAA 1201 TATTTGGGAC AAAAATCCAG TGCTTTATCT GCCACATCCG AAGCGGTCAA 1251 ACAACCGATG CTGCTTGACA ATAAAGTGCA ACATTACAGC TTTTTCGGTG 1301 TAGAACAGGC AAACTGGGAC AACTTCACGC TTGAAGGCGG CGTACGCGTG 1351 GAAAAACAAA AAGCCTCCAT CCGCTACGAC AAAGCATTGA TTGATCGGGA 1401 AAACTACTAC AACCATCCCC TGCCCGACCT CGGCGCGCAC CGCCAAACCG 1451 CCCGCTCATT CGCACTTTCG GGCAACTGGT ATTTCACGCC ACAACACAAA 1501 CTCAGCCTGA CCGCCTCCCA TCAGGAACGC CTGCCGTCAA CGCAAGAGCT 1551 GTACGCACAC GGCAAACACG TCGCCACCAA CACCTTTGAA GTCGGCAACA 1601 AACACCTCAA CAAAGAGCGT TCCAACAATA TCGAACTCGC GCTGGGCTAC 1651 GAAGGCGACC GCTGGCAATA CAATCTGGCA CTCTACCGCA ACCGCTTCGG 1701 CAACTACATT TACGCCCAAA CCTTAAACGA CGGACGCGGC CCCAAATCCA 1751 TCGAAGACGA CAGCGAAATG AAGCTCGTGC GCTACAACCA ATCCGGTGCG 1801 GACTTCTACG GCGCGGAAGG CGAAATCTAC TTCAAACCGA CACCGCGCTA 1851 CCGCATCGGC GTTTCCGGCG ACTATGTACG AGGCCGTCTG AAAAACCTGC 1901 CTTCCCTACC CGGCAGGGAA GACGCCTACG GCAACCGCCC ACTCATTGCC 1951 CAAGCCGACC AAAACGCCCC TCGCGTTCCG GCTGCGCGCC TCGGCGTCCA 2001 CCTGAAAGCC TCGCTGACCG ACCGCATCGA TGCCAATTTG GACTACTACC 2051 GCGTGTTCGC CCAAAACAAA CTCGCCCGCT ACGAAACGCG CACGCCCGGA 2101 CACCATATGC TCAACCTCGG CGCAAACTAC CGCCGCAATA CGCGCTATGG 2151 CGAGTGGAAT TGGTACGTCA AAGCCGACAA CCTGCTCAAC CAATCCGTTT 2201 ACGCCCACAG CAGCTTCCTC TCTGATACGC CGCAAATGGG CCGCAGCTTT 2251 ACCGGCGGCG TGAACGTGAA GTTTTAA This corresponds to the amino acid sequence <SEQ ID 606; ORF 149-1. a>: al49-l.pep 1 MAQTTLKPIV LSILLIΝTPL LSQAHGTEQS VGLETVSWG KSRPRATSGL 51 LHTSTASDKI ISGDTLRQKA VΝLGDALDGV PGIHASQYGG GASAPVIRGQ 101 TGRRIKVLΝH HGETGDMADF SPDHAIMVDS ALSQQVEILR GPVTLLYSSG 151 ΝVAGLVDVAD GKIPEKMPEΝ GVSGELGLRL SSGΝLEKLTS GGIΝIGLGKΝ 201 FVLHTEGLYR KSGDYAVPRY RΝLKRLPDSH ADSQTGSIGL SWVGEKGFIG 251 AAYSDRRDQY GLPAHSHEYD DCHADIIWQK SLIΝKRYLQL YPHLLTEEDI 301 DYDΝPGLSCG FHDDDDAHAH AHΝGKPWIDL RΝKRYELRAE WKQPFPGFEA 351 LRVHLNRNDY RHDEKAGDAV ENFFNNQTQN ARIELRHQPI GRLKGSWGVQ 401 YLGQKSΞALS ATSEAVKQPM LLDNKVQHYS FFGVEQANWD NFTLEGGVRV 451 EKQKASIRYD KALIDRENYY NHPLPDLGAH RQTARSFALS GNWYFTPQHK 501 LSLTASHQER LPSTQELYAH GKHVATNTFE VGNKHLNKER SNNIELALGY 551 EGDRWQYNLA LYRNRFGNYI YAQTLNDGRG PKSIEDDΞEM KLVRYNQSGA 601 DFYGAEGEIY FKPTPRYRIG VSGDYVRGRL KNLPSLPGRE DAYGNRPLIA 651 QADQNAPRVP AARLGVHLKA SLTDRIDANL DYYRVFAQNK LARYETRTPG 701 HHMLNLGANY RRNTRYGEWN WYVKADNLLN QSVYAHSSFL SDTPQMGRSF 751 TGGVNVKF* a!49- 'l/m.149-1 98.0% identity in 758 aa overlap 10 20 30 40 50 60 al49-l.pep MAQTTLKPIVLSILLINTPLLSQAHGTEQSVGLETVSVVGKSRPRATΞGLLHTSTASDKI I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml49-l MAQTTLKPIVLSILLINTPLLAQAHETEQSVDLETVΞVVGKSRPRATSGLLHTSTASDKI 10 20 30 40 50 60 70 80 90 100 110 120 al49-l.pep ISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTGRRIKVLNHHGETGDMADF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml49-1 ISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTGRRIKVLNHHGETGDMADF 70 80 90 100 110 120 130 140 150 160 170 180 al49-1. pep SPDHAIMVDΞALSQQVEILRGPVTLLYΞΞGNVAGLVDVADGKIPEKMPENGVSGELGLRL I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml49-l SPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVADGKIPEKMPENGVSGELGLRL 130 140 150 160 170 180 190 200 210 220 230 240 al49-l.pep ΞSGNLEKLTSGGINIGLGKNFVLHTEGLYRKSGDYAVPRYRNLKRLPDSHADSQTGSIGL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I ml49-l SΞGNLEKLTSGGINIGLGKNFVLHTEGLYRKΞGDYAVPRYRNLKRLPDSHADSQTGΞIGL 190 200 210 220 230 240 250 260 270 280 290 300 al49-l.pep SWVGEKGFIGAAYSDRRDQYGLPAHSHEYDDCHADIIWQKΞLINKRYLQLYPHLLTEEDI I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml49-l SWVGEKGFIGVAYSDRRDQYGLPAHSHEYDDCHADIIWQKSLINKRYLQLYPHLLTEEDI 250 260 270 280 290 300 310 320 330 340 350 360 al49-1.pep DYDNPGLΞCGFHDDDDAHAHAHNGKPWIDLRNKRYELRAEWKQPFPGFEALRVHLNRNDY I I I I I I I I I I I I I I I :| I I I : I : I :| I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml49-l DYDNPGLSCGFHDDDNAHAHTHSGRPWIDLRNKRYELRAEWKQPFPGFEALRVHLNRNDY 310 320 330 340 350 360 370 380 390 400 410 420 al49-l.pep RHDEKAGDAVENFFNNQTQNARIELRHQPIGRLKGSWGVQYLGQKSSALSATSEAVKQPM I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml49-l RHDEKAGDAVENFFNNQTQNARIELRHQPIGRLKGSWGVQYLQQKSΞALSAISEAVKQPM 370 380 390 400 410 420 430 440 450 460 470 480 al49-l.pep LLDNKVQHYSFFGVEQANWDNFTLEGGVRVEKQKAΞIRYDKALIDRENYYNHPLPDLGAH I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I ml49-l LLDNKVQHYSFFGVEQANWDNFTLEGGVRVEKQKASIQYDKALIDRENYYNHPLPDLGAH 430 440 450 460 470 480 490 500 510 520 530 540 al49-l.pep RQTARSFALΞGNWYFTPQHKLSLTASHQERLPSTQELYAHGKHVATNTFEVGNKHLNKER I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml49-l RQTARSFALSGNWYFTPQHKLSLTASHQERLPSTQELYAHGKHVATNTFEVGNKHLNKER 490 500 510 520 530 540 550 560 570 580 590 600 al49-l.pep SNNIELALGYEGDRWQYNLALYRNRFGNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml49-l SNNIELALGYEGDRWQYNLALYRNRFGNYIYAQTLNDGRGPKSIEDDΞEMKLVRYNQSGA 550 560 570 580 590 600 610 620 630 640 650 660 al49-l.pep DFYGAEGEIYFKPTPRYRIGVSGDYVRGRLKNLPSLPGREDAYGNRPLIAQADQNAPRVP I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I llllllll ml49-l DFYGAEGEIYFKPTPRYRIGVSGDYVRGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVP 610 620 630 640 650 660 670 680 690 700 710 720 al49-l.pep AARLGVHLKASLTDRIDANLDYYRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I ml49-l AARLGFHLKASLTDRIDANLDYYRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWN 670 680 690 700 710 720 730 740 750 759 al49-l .pep WYVKADNLLNQSVYAHΞΞFLSDTPQMGRSFTGGVNVKFX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml49-l WYVKADNLLNQSVYAHΞΞFLSDTPQMGRSFTGGVNVKFX 730 740 750 The following partial DNA sequence was identified in N.gonorrhoeae <SEQ ID 607>: gl50.seq (partial) 1 ..TACTGCAAGG CAGACCCCTT TCCCGCCGCC CTGCTGGCCA ATCAGAAAAT 51 CACCGCCCGC CAATCCGATA AAGACGTGCG CCACATCGAA ATCGATTTGA 101 GCGGTTCGGA TTTGCACTAC CTCCCGGGCG ACGCGCTCGG CGTTTGGTTT 151 GACAACGATC CGGCACTGGT CGGGGAAATC CTAGACCTGC TCGGCATCAA 201 TCCGGCAACG GAAATACAGG CGGGCGGAAA AACCCTGCCG GTTGCCTCCG 251 CACTGTTATC CCATTTCGAA CTCACGCAAA ACACCCCCGC CTTTGTCAAA 301 GGCTATGCCA CGTTCGCCGA TAATGACGAA CTCGACCGTA TTGCTGCCGA 351 CAACGCCGTT TTGCAAGGCT TTGTGCAAAG CACGCCGATT GCCGGTGTGC 401 TGCACCGCTT CCCGGCAAAA CTGACGGCGG AACAATTCGC CGGCCTGCTG 451 CGCCCGCTTG CGCCGCGCCT GTATTCGATT TCCTCGTCGC AGGCGGAAGC 501 GGGGGACGAA GTGCACCTGA CCGTCGGCGC AGTGCGTTTC GAACACGAAG 551 GGCGCGCCAG GGCGGGCGGC GCATCGGGTT TCTTTGCCGA CCGGCTGGAA 601 GAGGACGGCA CGGTGCGCGT GTTTGCGGAA CGCAACGACG GCTTCAGGCT 651 GCCCGAAGAC AGCCGCAAGC CGATTGTGAT GATCGGCTCC GGTACCGGCG 701 TCGCACCGTT CCGCGCCTTC GTCCAACAAC GTGCCGCAGA AAATGCGGAA 751 GGCAGAAACT GGCTGATTTT CGGCAATCCG CATTTTGCCG CCGACTTCCT 801 CTATCAGACC GAATGGCAGC AGTTTGCCAA AGACGGCTTC CTGCACAGAT 851 ATGACTTCGC CTGGTCGCGC GATCAGGAAG AAAAAATCTA TGTGCAGGAC 901 AAAATCCGCG AACAGGCGGA AGGACTTTGG CAATGGCTGC AGGAAGGCGC 951 GCATATCTAT GTGTGCGGCG ATGCGGCAAA AATGGCAAAA GAAGTGGAAG 1001 CCGCCTTGCT GGATGTGATT ATCGGGGCAG GGCATTCGGA CGAAGACGGC 1051 GCAGAAGGAT ATTTGGATAT GCTGCGCGAA GAAAAACGCT ATCAGCGTGA 1101 TGTTTATTGA This corresponds to the amino acid sequence <SEQ ID 608; ORF 150.ng>: gl50.pep (partial) 1 ..YCKADPFPAA LLANQKITAR QSDKDVRHIE IDLSGSDLHY LPGDALGVWF 51 DNDPALVGEI LDLLGINPAT EIQAGGKTLP VASALLSHFE LTQNTPAFVK 101 GYATFADNDE LDRIAADNAV LQGFVQSTPI AGVLHRFPAK LTAEQFAGLL 151 RPLAPRLYSI SSSQAEAGDE VHLTVGAVRF EHEGRARAGG ASGFFADRLE 201 EDGTVRVFAE RNDGFRLPED SRKPIVMIGS GTGVAPFRAF VQQRAAENAE 251 GRNWLIFGNP HFAADFLYQT EWQQFAKDGF LHRYDFAWSR DQEEKIYVQD 301 KIREQAEGLW QWLQEGAHIY VCGDAAKMAK EVEAALLDVI IGAGHSDEDG 351 AEGYLDMLRE EKRYQRDVY* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 609>: ml50. seq 1 ATGCAGAACA CAAATCCGCC ATTACCGCCT CTGCCGCCCG AAATCACGCA 51 GCTCCTGTCG GGGCTGGACG CGGCACAATG GGCGTGGCTG TCCGGCTACG 101 CTTGGGCAAA AGCAGGAAAC GGGGCATCTG CAGGACTGCC CGCGCTTCAG 151 ACGGCATTGC CGGCGGCAGA ACCTTTTTCC GTAACCGTCC TTTCCGCCTC 201 GCAAACCGGC AATGCGAAAT CCGTTGCCGA CAAAGCGGCG GACAGCCTGG 251 AAGCCGCCGG CATCCAAGTC AGTCGCGCCG AACTGAAAGA CTATAAGGCG 301 AAAAACATCG CCGGCGAACG CCGCCTGCTG CTGGTTACCT CCACCCAAGG 351 CGAAGGCGAA CCGCCGAAAG AAGCCGTCGT GCTGCACAAA CTGCTGAACG 401 GCAAAAAAGC CCCGAAATTG GACAAACTCC AATTTGCCGT ACTGGGTTTG 451 GGCGACAGTT CCTATCCGAA TTTCTGTCAG GCAGGTAAAG ATTTCGACCG 501 GCGTTTTGAA GAATTGGGCG CAAAACGGCT GCTCGAACGC GTTGATGCGG 551 ATTTGGACTT TACCGCCTCC GCAAACGCCT GGACAGATAA TATCGCCGCA 601 CTCTTAAAAG AAGAAGCCGC AAAAAACCGG GCAACGCCCG CGCCGCAGAC 651 AACGCCCCCC GCCGGCCTTC AGACGGCACC GGATGGCAGG TACTGCAAGG 701 CAGCCCCCTT TCCCGCCGCC CTGCTGGCCA ATCAGAAAAT CACCGCCCGC 751 CAATCCGATA AAGACGTGCG CCACATCGAA ATCGATTTGA GCGGTTCGGA 801 TTTGCACTAC CTCCCGGGCG ACGCGCTCGG CGTTTGGTTT GACAACGATC 851 CGGCACTGGT CAGGGAAATC CTAGACCTGC TCGGCATCGA TCCGGCAACG 901 GAAATACAGG CGGGCGGAAA GATGATGCCG GTTGCGCGCG CACTTTCATC 951 TCATTTCGAA CTCACGCAAA ACACTCCGGC TTTCGTCAAA GGCTATGCCG 1001 CGTTCGCCCA TTATGAAGAA CTCGATAAAA TCATTGCCGA TAACGCCGTT 1051 TTGCAGGATT TCGTGCAAAA CACGCCTATT GTCGATGTGC TGCACCGCTT 1101 CCCGGCAAGC CTGACGGCAG AACAATTCAT CCGTTTACTG CGTCCGCTTG 1151 CACCCCGTTT GTATTCGATT TCTTCAGCAC AGGCGGAAGT GGGCGATGAA 1201 GTGCATTTAA CTGTCGGCGT GGTTCGTTTT GAACACGAAG GCCGCGCCAG 1251 AACGGGCGGC GCATCGGGTT TCCTTGCCGA CCGGCTGGAA GAGGACGGCA 1301 CGGTGCGCGT GTTTGTGGAA CGCAACGACG GCTTCAGGCT GCCCGAAGAC 1351 AGCCGCAAGC CGATTGTGAT GATCGGCTCG GGCACCGGCG TCGCACCGTT 1401 CCGCGCTTTC GTCCAACAAC GTGCCGCAGA AAATGCGGAA GGCAAAAACT 1451 GGCTGATTTT CGGCAATCCG CATTTTGCCC GTGATTTTCT CTATCAAACC 1501 GAATGGCAGC AGTTTGCCAA AGACGGCTTC CTGCACAGGT ACGATTTCGC 1551 CTGGTCCCGC GATCAGGAAG AAAAAATCTA TGTGCAGGAC AAAATCCGCG 1601 AACAGGCGGA AGGACTTTGG CAATGGCTGC AGGAAGGCGC GCATATCTAT 1651 GTGTGCGGCG ATGCGGCAAA AATGGCAAAA GACGTGGAAG CCGCCTTGCT 1701 GGATGTGATT ATCGGGGCAG GACATTTGGA CGAAGAGGGC GCAGAAGAAT 1751 ATTTGGATAT GCTGCGCGAA GAAAAACGCT ATCAGCGTGA TGTTTATTGA This corresponds to the amino acid sequence <SEQ LD 610; ORF 150>: ml50.pep 1 MQNTNPPLPP LPPEITQLLS GLDAAQWAWL SGYAWAKAGN GASAGLPALQ 51 TALPAAEPFS VTVLSASQTG NAKSVADKAA DSLEAAGIQV SRAELKDYKA 101 KNIAGERRLL LVTSTQGEGE PPKEAWLHK LLNGKKAPKL DKLQFAVLGL 151 GDSSYPNFCQ AGKDFDRRFE ELGAKRLLER VDADLDFTAS ANAWTDNIAA 201 LLKEEAAKNR ATPAPQTTPP AGLQTAPDGR YCKAAPFPAA LLANQKITAR 251 QSDKDVRHIE IDLSGSDLHY LPGDALGVWF DNDPALVREI LDLLGIDPAT 301 EIQAGGKMMP VARALSSHFE LTQNTPAFVK GYAAFAHYEE LDKIIADNAV 351 LQDFVQNTPI VDVLHRFPAS LTAEQFIRLL RPLAPRLYSI SSAQAEVGDE 401 VHLTVGWRF EHEGRARTGG ASGFLADRLE EDGTVRVFVE RNDGFRLPED 451 SRKPIVMIGS GTGVAPFRAF VQQRAAENAE GKNWLIFGNP HFARDFLYQT 501 EWQQFAKDGF LHRYDFAWSR DQEEKIYVQD KIREQAEGLW QWLQEGAHIY 551 VCGDAAKMAK DVEAALLDVI IGAGHLDEEG AEEYLDMLRE EKRYQRDVY* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 150 shows 91.3% identity over a 369 aa overlap with a predicted ORF (ORF 150.ng) from N gonorrhoeae: ml50/gl50 210 220 230 240 250 260 ml50.pep LLKEEAAKΝRATPAPQTTPPAGLQTAPDGRYCKAAPFPAALLAΝQKITARQSDKDVRHIE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl50 YCKADPFPAALLAΝQKITARQSDKDVRHIE 10 20 30 270 280 290 300 310 320 ml50.pep IDLSGSDLHYLPGDALGVWFDΝDPALVREILDLLGIDPATEIQAGGKMMPVARALSSHFE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I : I I I II M I I gl50 IDLSGSDLHYLPGDALGVWFDΝDPALVGEILDLLGIΝPATEIQAGGKTLPVASALLSHFE 40 50 60 70 80 90 330 340 350 360 370 380 ml50. pep LTQΝTPAFVKGYAAFAHYEELDKIIADΝAVLQDFVQΝTPIVDVLHRFPASLTAEQFIRLL I I I I I I I I I I I I I : I I : I I I : I l l l l l l l I I I : I I I : I I I I I I I : I I I I I I I I gl50 LTQNTPAFVKGYATFADNDELDRIAADNAVLQGFVQSTPIAGVLHRFPAKLTAEQFAGLL 100 110 120 130 140 150 390 400 410 420 430 440 ml50 . pep RPLAPRLYSISSAQAEVGDEVHLTVGWRFEHEGRARTGGASGFLADRLEEDGTVRVFVE I I I I I I I I I I I I : I I I: I I I I I I I I I: I I I I I I I I I I: I I I I II : II I I I I I I I I I I I : I g150 RPLAPRLYSISSSQAEAGDEVHLTVGAVRFEHEGRARAGGASGFFADRLEEDGTVRVFAE 160 170 180 190 200 210 450 460 470 480 490 500 ml50. pep RNDGFRLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAEGKNWLIFGNPHFARDFLYQT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I llllll gl50 RNDGFRLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAEGRNWLIFGNPHFAADFLYQT 220 230 240 250 260 270 510 520 530 540 550 560 ml50. pep EWQQFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I gl50 EWQQFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAK 280 290 300 310 320 330 570 580 590 600 ml50. pep DVEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVYX : I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I gl50 EVEAALLDVIIGAGHSDEDGAEGYLDMLREEKRYQRDVYX 340 350 360 370 The following partial DNA sequence was identified in N meningitidis <SEQ ID 611>: al50. seq 1 ATGCAGAACA CAAATCCGCC ATTACCGCCT ATGCCGCCCG AAATCACGCA 51 GCTCCTGTCG GGGCTGGACG CGGCACAATG GGCGTGGCTG TCCGGCTACG 101 CTTGGGCAAA AGCAGGAAAC GGGGCATCTG CAGGACTGCC CGCGCTTCAG 151 ACGGCATTGC CGACGGCAGA ACCTTTTTCC GTAACCGTCC TTTCCGCCTC 201 GCAAACCGGC AATGCGAAAT CCGTTGCCGA CAAAGCGGCG GACAGCCTGG 251 AAGCCGCCGG CATCCAAGTC AGTCGCGCCG AACTGAAAGA CTATAAGGCG 301 AAAAACATCG CCGGCGAACG CCGCCTGCTG CTGGTTACCT CCACCCAAGG 351 CGAAGGCGAA CCGCCGGAAG AAGCCGTCGT GCTGCACAAA CTGCTGAACG 401 GCAAAAAAGC CCCGAAATTG GACAAACTCC AATTTGCCGT ACTGGGTTTG 451 GGCGACAGCT CCTATCCGAA TTTCTGCCGG GCGGGCAAAG ATTTCGACAA 501 ACGTTTTGAA GAATTGGGCG CAAAACGCCT GCTCGAACGC GTTGATGCGG 551 ATTTGGACTT TGCCGCCGCC GCAGACGGAT GGACAGATAA TATCGCCGCA 601 CTCTTAAAAG AAGAAGCCGC AAAAAACCGG GCAACGCCCG CGCCGCAGAC 651 AACGCCCCCC GCCGGCCTTC AGACGGCACC GGATGGCAGG TACTGCAAGG 701 CAGACCCCTT TCCCGCCGCC CTGCTGGCCA ATCAGAAAAT CACCGCCCGC 751 CAATCCGATA AAGACGTGCG CCACATCGAA ATCGATTTGA GCGGTTCGGA 801 TTTGCACTAC CTCCCGGGCG ACGCGCTCGG CGTTTGGTTT GACAACGATC 851 CGGCACTGGT CAGGGAAATC CTAGACCTGC TCGGCATCGA TCAGGCAACG 901 GAAATACAGG CGGGCGGAAA AACCCTGCCG GTTGCCTCCG CACTGTTATC 951 CCATTTTGAA CTCACGCAAA ACACCCCCGC CTTTGTCAAA GGCTATGCCC 1001 CGTTCGCCGA TGATGACGAA CTCGACCGTA TTGCTGCCGA CAACGCCGTT 1051 TTGCAAGGCT TTGTGCAAAG CACGCCGATT GCCGATGTGC TGCACCGCTT 1101 CCCGGCAAAA CTGACAGCGG AACAATTCGC CGGCCTACTG CGCCCGCTTG 1151 CGCCGCGCCT GTATTCGATT TCCTCGTCGC AGGCGGAAGT GGGGGACGAA 1201 GTGCACCTGA CCGTCGGCGC GGTGCGTTTC GAACACGAAG GGCGCGCCAG 1251 GGCGGGCGGC GCATCGGGTT TCCTTGCCGA CCGGCTGGAA GAGGACGGCA 1301 CGGTGCGCGT GTTTGTGGAA CGCAACGACG GCTTCAGGCT GCCCGAAGAC 1351 AGCCGCAAGC CGATTGTGAT GATCGGCTCG GGCACCGGCG TCGCACCGTT 1401 CCGCGCTTTC GTCCAACAAC GTGCCGCAGA AAATGCGGAA GGCAAAAACT 1451 GGCTGTTTTT CGGCAATCCG CATTTTGCCC GTGATTTTCT CTATCAAACC 1501 GAATGGCAGC AGTTTGCCAA AGACGGCTTC CTGCACAGAT ACGATTTCGC 1551 CTGGTCGCGC GATCAGGAAG AAAAAATCTA TGTGCAGGAC AAAATCCGCG 1601 AACAGGCGGA AGGACTTTGG CAATGGCTGC AGGAAGGCGC GCATATCTAT 1651 GTGTGCGGCG ATGCGGCAAA AATGGCAAAA GACGTGGAAG CCGCCTTGCT 1701 GGATGTGATT ATCGGGGCAG GACATTTGGA CGAAGAGGGC GCAGAAGAAT 1751 ATTTGGATAT GCTGCGCGAA GAAAAACGCT ATCAGCGTGA TGTTTATTGA This corresponds to the amino acid sequence <SEQ ID 612; ORF 150.a>: al50.pep 1 MQNTNPPLPP MPPEITQLLS GLDAAQWAWL SGYAWAKAGN GASAGLPALQ 51 TALPTAEPFS VTVLSASQTG NAKSVADKAA DSLEAAGIQV SRAELKDYKA 101 KNIAGERRLL LVTSTQGEGE PPEEAWLHK LLNGKKAPKL DKLQFAVLGL 151 GDSSYPNFCR AGKDFDKRFE ELGAKRLLER VDADLDFAAA ADGWTDNIAA 201 LLKEEAAKNR ATPAPQTTPP AGLQTAPDGR YCKADPFPAA LLANQKITAR 251 QSDKDVRHIE IDLSGSDLHY LPGDALGVWF DNDPALVREI LDLLGIDQAT 301 EIQAGGKTLP VASALLSHFE LTQNTPAFVK GYAPFADDDE LDRIAADNAV 351 LQGFVQSTPI ADVLHRFPAK LTAEQFAGLL RPLAPRLYSI SSSQAEVGDE 401 VHLTVGAVRF EHEGRARAGG ASGFLADRLE EDGTVRVFVE RNDGFRLPED 451 SRKPIVMIGS GTGVAPFRAF VQQRAAENAE GKNWLFFGNP HFARDFLYQT 501 EWQQFAKDGF LHRYDFAWSR DQEEKIYVQD KIREQAEGLW QWLQEGAHIY 551 VCGDAAKMAK DVEAALLDVI IGAGHLDEEG AEEYLDMLRE EKRYQRDVY* ml50/al50 94.8% identity in 599 aa overlap 10 20 30 40 50 60 ml50. pep MQNTNPPLPPLPPEITQLLΞGLDAAQWAWLSGYAWAKAGNGAΞAGLPALQTALPAAEPFS I I I I I I I I I I :| I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I al50 MQNTNPPLPPMPPEITQLLSGLDAAQWAWLSGYAWAKAGNGAΞAGLPALQTALPTAEPFS 10 20 30 40 50 60 70 80 90 100 110 120 ml50. pep VTVLSASQTGNAKSVADKAADSLEAAGIQVSRAELKDYKAKNIAGERRLLLVTΞTQGEGE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al50 VTVLSAΞQTGNAKSVADKAADSLEAAGIQVSRAELKDYKAKNIAGERRLLLVTSTQGEGE 70 80 90 100 110 120 130 140 150 160 170 180 l50. .pep PPKEAWLHKLLNGKKAPKLDKLQFAVLGLGDSSYPNFCQAGKDFDRRFEELGAKRLLER I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : l I I I I 1 : 1 I I I I I I I I I I I I al50 PPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDΞΞYPNFCRAGKDFDKRFEELGAKRLLER 130 140 150 160 170 180 190 200 210 220 230 240 ml50. ,pep VDADLDFTASANAWTDNIAALLKEEAAKNRATPAPQTTPPAGLQTAPDGRYCKAAPFPAA I I I I I I I :| :| :: I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al50 VDADLDFAAAADGWTDNIAALLKEEAAKNRATPAPQTTPPAGLQTAPDGRYCKADPFPAA 190 200 210 220 230 240 250 260 270 280 290 300 ml50. .pep LLANQKITARQSDKDVRHIEIDLSGSDLHYLPGDALGVWFDNDPALVREILDLLGIDPAT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I II al50 LLANQKITARQSDKDVRHIEIDLΞGΞDLHYLPGDALGVWFDNDPALVREILDLLGIDQAT 250 260 270 280 290 300 310 320 330 340 350 360 ml50. .pep EIQAGGKMMPVARALSSHFELTQNTPAFVKGYAAFAHYEELDKIIADNAVLQDFVQNTPI lllllll : I I I II I I I I I I I I I I I I I I I I I II :| I I :| lllllll I I I : I I I al50 EIQAGGKTLPVASALLSHFELTQNTPAFVKGYAPFADDDELDRIAADNAVLQGFVQSTPI 310 320 330 340 350 360 370 380 390 400 410 420 ral50. .pep VDVLHRFPAΞLTAEQFIRLLRPLAPRLYSISSAQAEVGDEVHLTVGWRFEHEGRARTGG : I I I I I I I I :| I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I : I I I I I I I I I I : I I al50 ADVLHRFPAKLTAEQFAGLLRPLAPRLYSISSSQAEVGDEVHLTVGAVRFEHEGRARAGG 370 380 390 400 410 420 430 440 450 460 470 480 ml50.pep AΞGFLADRLEEDGTVRVFVERNDGFRLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al50 ASGFLADRLEEDGTVRVFVERNDGFRLPEDSRKPIVMIGΞGTGVAPFRAFVQQRAAENAE 430 440 450 460 470 480 490 500 510 520 530 540 ml50. pep GKNWLIFGNPHFARDFLYQTEWQQFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLW I I I I I :| I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al50 GKNWLFFGNPHFARDFLYQTEWQQFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLW 490 500 510 520 530 540 550 560 570 580 590 600 ml50. pep QWLQEGAHIYVCGDAAKMAKDVEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVYX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a150 QWLQEGAHIYVCGDAAKMAKDVEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVYX 550 560 570 580 590 600 The following partial DNA sequence was identified in N.gonorrhoeae <SEQ ID 613>: gl51.seq 1 ATGAAACAAA TCCGCAACAT CGCCATCATC GCACACGTCG ACCACGGCAA 51 AACCACATTG GTCGACCAAC TGCTGCGCCA ATCCGGCACA TTCCGCGCCA 101 ACCAGCAGGT TGACGAGCGC GTGATGGACA GCAACGACCT TGAAAAAGAA 151 CGCGGCATCA CCATCCTCGC CAAAAACACC GCCATCGATT ACGAAGGCTG 201 CCACATCAAT ATCGTCGACA CGCCGGGACA CGCCGACTTC GGCGGCGAAG 251 TGGAGCGCGT TTTGGGGATG GTGGATTGCG TCGTCTTGTT GGTGGACGCA 301 CAGGAAGGTC CGATGCCGCA AACCCGTTTC GTGACCAAAA AAGCCTTGGC 351 TTTGGGGCTG AAACCGATTG TCGTCATCAA CAAAATCGAC AAACCGTCCG 401 CCCGTCCGAG CTGGGTTATC GACCAGACTT TCGAGTTGTT CGACAACTTG 451 GGTGCGACCG ACGAGCAGTT GGATTTCCCG ATTGTTTACG CTTCAGGTTT 501 GAGCGGCTTT GCCAAGCTGG AAGAAAccga CGAGAGCAGC GATATGCGCC 551 CGCtgttcgA CACCATCCTA AAATACAcgc ctgCACCGAG CGGCAGCGCG 601 GACGAGCCGC TGCAACTGCA AATTTCCCAA CTCGACTACG ACAACTACAC 651 CGGCCGCCTC GGTATCGGTC GTATCTTGAA CGGACGCATC AAACCCGGCC 701 AAACCGTTGC CGTGATGAAC CACGAGCAGC AAATCGCCCA AGGCCGCATC 751 AACCAGCTTT TGGGTTTCAA AGGCTTGGAA CGCGTGCCGC TTGAAGAAGC 801 CGAAGCCGGC GACATTGTGA TTATTTCCGG TATCGAAGAC ATCGGCATCG 851 GCGTAACCAT CACCGACAAA GACAACCCCA AAGGCCTGCC GATGTTGAGC 901 GTGGACGAAC CGACGCTGAC GATGGACTTT ATGGTAAACA CCAGCCCGCT 951 CGCAGGTACA GAAGGCAAAT TCGTGACCAG CCGCCAAATC CGCGACCGCC 1001 TGCAAAAAGA ATTGCTGACC AACGTTGCCC TGCGCGTGGA AGACACCGCC 1051 GatgCCGACG TGTTCCGCGT ATCcgGGCGC GGCGAACTGC ACCTGACGAT 1101 TTTGCTGGAA AATATGCGCC GCGAAGGCTA CGAACTCGCC GTCGGCAAGC 1151 CGCGCGTCGT GTACCGAGAC ATCGACGGTC AAAAATGCGA ACCTTATGAA 1201 AACCTGACTG TGGACGTACc cgacgacAAC CAAGGCGCGG TAATGGAAGA 1251 ACTCGGCCGC CGCCGTGGCG AACTGACCAA TATGGAAAGC GACGGCAACG 1301 GacgCACCCG CCTCGAATAC CATATTCCAG CGCGCGGCTT GATCGGTTTC 1351 CAAGGCGAAT TCATGACCCT GACGCGCGGC GTCGGGCTGA TGAgccacGT 1401 GTTcgacgac tacgcgcccg tcaAACCCGA TATGCCCGGC CGCCACAACG 1451 GCGTactggt GtcccaAGAG CAGGGCGAGG CGGTTGCTTA CGCCTTGTGG 1501 AATCTTGAAG ACCGCGGCCG TATGTTCGTA TCGCCCAACG ACAAAATCTA 1551 CGAAGGTATG ATTATCGGCA TCCACAGCCG CGACAACGAT TTGGTGGTCA 1601 ACCCGCTCAA AGGCAAAAAA CTCACCAATA TCCGTGCCAG CGGTACCGAC 1651 GAAGCGGTGC GCCTGACCAC GCCGATCAAA CTGAcgcTGG AAGGCGCGGT 1701 CGAGTTTATC GACGATGACG AGCTGGTGGA AATCACGCCG CAAtCCatCC 1751 gcctgcgcat gcgttacctG AGCGaattgg aacgccgccg tcaTTTTAAA 1801 AagctgGATT AA This corresponds to the amino acid sequence <SEQ ID 614; ORF 151.ng>: glδl.pep 1 MKQIRNIAII AHVDHGKTTL VDQLLRQSGT FRANQQVDER VMDSNDLEKE 51 RGITILAKNT AIDYEGCHIN IVDTPGHADF GGEVERVLGM VDCWLLVDA 101 QEGPMPQTRF VTKKALALGL KPIWINKID KPSARPSWVI DQTFELFDNL 151 GATDEQLDFP IVYASGLSGF AKLEETDESS DMRPLFDTIL KYTPAPSGSA 201 DEPLQLQISQ LDYDNYTGRL GIGRILNGRI KPGQTVAVMN HEQQIAQGRI 251 NQLLGFKGLE RVPLEEAEAG DIVIISGIED IGIGVTITDK DNPKGLPMLS 301 VDEPTLTMDF MVNTSPLAGT EGKFVTSRQI RDRLQKELLT NVALRVEDTA 351 DADVFRVSGR GELHLTILLE NMRREGYELA VGKPRWYRD IDGQKCEPYE 401 NLTVDVPDDN QGAVMEELGR RRGELTNMES DGNGRTRLEY HIPARGLIGF 451 QGEFMTLTRG VGLMSHVFDD YAPVKPDMPG RHNGVLVSQE QGEAVAYALW 501 NLEDRGRMFV SPNDKIYEGM IIGIHSRDND LWNPLKGKK LTNIRASGTD 551 EAVRLTTPIK LTLEGAVEFI DDDELVEITP QSIRLRMRYL SELERRRHFK 601 KLD* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 615>: ml51.seq 1 ATGAAACAAA TCCGCAACAT CGCCATCATC GCCCACGTCG ACCACGGCAA 51 AACCACATTG GTCGACCAAC TGCTGCGCCA ATCCGGCACA TTCCGCGCCA 101 ACCAGCAGGT TGACGAGCGC GTGATGGACA GCAACGACCT TGAAAAAGAA 151 CGCGGCATCA CCATCCTCGC CAAAAACACC GCCATCGATT ACGAAGGCTA 201 CCACATCAAT ATCGTCGACA CGCCGGGACA CGCCGACTTC GGCGGCGAAG 251 TAGAGCGCGT TTTGGGGATG GTGGACTGCG TCGTCTTGTT GGTGGACGCG 301 CAGGAAGGCC CGATGCCGCA AACCCGTTTC GTGACCAAAA AAGCCTTGGC 351 TTTGGGGCTG AAACCGATTG TCGTCATCAA CAAAATCGAC AAGCCGTCCG 401 CTCGTCCGAG CTGGGTTATC GACCAAACTT TCGAGCTGTT CGACAACTTG 451 GGCGCGACCG ACGAGCAGTT GGATTTCCCG ATTGTTTACG CTTCAGGGTT 501 GAGCGGTTTC GCCAAATTGG AAGAAACCGA CGAGAGCAAC GACATGCGTC 551 CGCTGTTCGA TACTATCTTA AAATATACGC CTGCACCGAG CGGCAGCGCG 601 GACGAAACGC TGCAACTGCA AATTTCCCAA CTCGACTACG ACAACTACAC 651 CGGCCGCCTC GGTATCGGTC GTATCTTGAA CGGACGCATC AAACCCGGCC 701 AAACCGTTGC CGTCATGAAC CACGATCAGC AAATCGCCCA AGGCCGCATC 751 AACCAGCTTT TGGGTTTCAA AGGTTTGGAA CGCGTGCCGC TTGAAGAAGC 801 CGAAGCCGGC GACATCGTGA TTATTTCCGG TATCGAAGAC ATCGGTATCG 851 GCGTAACCAT CACCGACAAA GACAATCCCA AAGGCCTACC GATGTTGAGC 901 GTGGACGAAC CGACGCTGAC GATGGACTTT ATGGTCAACA CCAGCCCGCT 951 GGCGGGTACG GAAGGCAAAT TCGTAACCAG CCGCCAAATC CGCGACCGCC 1001 TGCAAAAAGA ATTGCTGACC AACGTCGCCC TGCGCGTGGA AGATACCGCC 1051 GATGCCGACG TGTTCCGCGT ATCCGGGCGC GGCGAGCTGC ACCTGACCAT 1101 TTTGCTGGAA AACATGCGCC GCGAAGGCTA CGAACTCGCC GTCGGCAAAC 1151 CGCGCGTCGT GTACCGCGAC ATCGACGGTC AAAAATGCGA ACCGTATGAA 1201 AACCTGACCG TGGATGTACC CGACGACAAC CAAGGCGCGG TAATGGAAGA 1251 ACTCGGCCGC CGCCGTGGCG AACTGACTAA TATGGAAAGC GACGGCAACG 1301 GACGCACCCG CCTCGAATAC CATATTCCAG CGCGCGGCTT GATCGGTTTC 1351 CAAGGCGAAT TTATGACCCT GACGCGCGGG GTCGGGCTGA TGAGCCACGT 1401 GTTCGACGAT TACGCGCCCG TCAAACCCGA TATGCCCGGC CGCCACAACG 1451 GCGTGCTGGT GTCCCAAGAG CAGGGCGAGG CAGTCGCTTA CGCCTTGTGG 1501 AATCTGGAAG ACCGCGGCCG TATGTTCGTA TCGCCCAACG ACAAAATCTA 1551 CGAAGGCATG ATTATCGGCA TCCACAGTCG CGACAACGAT TTGGTGGTCA 1601 ACCCGCTCAA AGGCAAAAAA CTTACCAACA TCCGTGCCAG CGGTACCGAC 1651 GAAGCCGTTC GCCTGACCAC GCCAATCAAG CTGACGCTGG AAGGTGCGGT 1701 TGAGTTTATC GACGATGACG AACTCGTTGA AATCACGCCG CAATCCATCC 1751 GTCTGCGCAA GCGTTACTTG AGCGAATTGG AACGCCGCCG CCACTTTAAA 1801 AAGCTGGATT GA This corresponds to the amino acid sequence <SEQ ID 616; ORF 151>: ml51.pep 1 MKQIRNIAII AHVDHGKTTL VDQLLRQSGT FRANQQVDER VMDSNDLEKE 51 RGITILAKNT AIDYEGYHIN IVDTPGHADF GGEVERVLGM VDCWLLVDA 101 QEGPMPQTRF VTKKALALGL KPIWINKID KPSARPSWVI DQTFELFDNL 151 GATDEQLDFP IVYASGLSGF AKLEETDESN DMRPLFDTIL KYTPAPSGSA 201 DETLQLQISQ LDYDNYTGRL GIGRILNGRI KPGQTVAVMN HDQQIAQGRI 251 NQLLGFKGLE RVPLEEAEAG DIVIISGIED IGIGVTITDK DNPKGLPMLS 301 VDEPTLTMDF MVNTSPLAGT EGKFVTSRQI RDRLQKELLT NVALRVEDTA 351 DADVFRVSGR GELHLTILLE NMRREGYELA VGKPRWYRD IDGQKCEPYE 401 NLTVDVPDDN QGAVMEELGR RRGELTNMES DGNGRTRLEY HIPARGLIGF 451 QGEFMTLTRG VGLMSHVFDD YAPVKPDMPG RHNGVLVSQE QGEAVAYALW 501 NLEDRGRMFV SPNDKIYEGM IIGIHSRDND LWNPLKGKK LTNIRASGTD 551 EAVRLTTPIK LTLEGAVEFI DDDELVEITP QSIRLRKRYL SELERRRHFK 601 KLD* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 151 shows 99.2% identity over a 603 aa overlap with a predicted ORF (ORF 15 l.ng) from N. gonorrhoeae: ml51/gl51 10 20 30 40 50 60 ml51.pep MKQIRΝIAIIAHVDHGKTTLVDQLLRQSGTFRAΝQQVDERVMDSΝDLEKERGITILAKΝT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl51 MKQIRΝIAIIAHVDHGKTTLVDQLLRQSGTFRAΝQQVDERVMDSΝDLEKERGITILAKΝT 10 20 30 40 50 60 70 80 90 100 110 120 ml51.pep AIDYEGYHIΝIVDTPGHADFGGEVERVLGMVDCWLLVDAQEGPMPQTRFVTKKALALGL llllll I I I I I I I I I I I I II I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I gl51 AIDYEGCHIΝIVDTPGHADFGGEVERVLGMVDCWLLVDAQEGPMPQTRFVTKKALALGL 70 80 90 100 110 120 130 140 150 160 170 180 ml51 . pep KPIWIΝKIDKPSARPSWVIDQTFELFDΝLGATDEQLDFPIVYASGLSGFAKLEETDESΝ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : gl51 KPIWIΝKIDKPSARPSWVIDQTFELFDΝLGATDEQLDFPIVYASGLSGFAKLEETDESS 130 140 150 160 170 180 190 200 210 220 230 240 ml51 . pep DMRPLFDTILKYTPAPSGSADETLQLQISQLDYDΝYTGRLGIGRILΝGRIKPGQTVAVMΝ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl51 DMRPLFDTILKYTPAPSGSADEPLQLQISQLDYDΝYTGRLGIGRILΝGRIKPGQTVAVMΝ 190 200 210 220 230 240 250 260 270 280 290 300 ml51. ■pep HDQQIAQGRIΝQLLGFKGLERVPLEEAEAGDIVIISGIEDIGIGVTITDKDΝPKGLPMLS I: I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl51 HEQQIAQGRIΝQLLGFKGLERVPLEEAEAGDIVIISGIEDIGIGVTITDKDΝPKGLPMLS 250 260 270 280 290 300 310 320 330 340 350 360 ml51, .pep VDEPTLTMDFMVΝTSPLAGTEGKFVTSRQIRDRLQKELLTΝVALRVEDTADADVFRVSGR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl51 VDEPTLTMDFMVΝTSPLAGTEGKFVTSRQIRDRLQKELLTΝVALRVEDTADADVFRVSGR 310 320 330 340 350 360 370 380 390 400 410 420 ml51. •pep GELHLTILLEΝMRREGYELAVGKPRWYRDIDGQKCEPYEΝLTVDVPDDΝQGAVMEELGR I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I gl51 GELHLTILLEΝMRREGYELAVGKPRWYRDIDGQKCEPYEΝLTVDVPDDΝQGAVMEELGR 370 380 390 400 410 420 430 440 450 460 470 480 ml51. ■pep RRGELTΝMESDGΝGRTRLEYHIPARGLIGFQGEFMTLTRGVGLMSHVFDDYAPVKPDMPG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl51 RRGELTΝMESDGΝGRTRLEYHIPARGLIGFQGEFMTLTRGVGLMSHVFDDYAPVKPDMPG 430 440 450 460 470 480 490 500 510 520 530 540 ml51. ■pep RHΝGVLVSQEQGEAVAYALWΝLEDRGRMFVSPΝDKIYEGMIIGIHSRDΝDLWΝPLKGKK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl51 RHΝGVLVSQEQGEAVAYALWΝLEDRGRMFVSPΝDKIYEGMIIGIHSRDΝDLVVΝPLKGKK 490 500 510 520 530 540 550 560 570 580 590 600 ml51.pep LTΝIRASGTDEAVRLTTPIKLTLEGAVEFIDDDELVEITPQSIRLRKRYLSELERRRHFK I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl51 LTΝIRASGTDEAVRLTTPIKLTLEGAVEFIDDDELVEITPQSIRLRMRYLSELERRRHFK 550 560 570 580 590 600 ml 51 . pep KLDX I I I I gl51 KLDX The following partial DNA sequence was identified in N. meningitidis <SEQ ID 617>: al51. seq 1 ATGAAACAAA TCCGCAACAT CGCCATCATC GCCCACGTCG ACCACGGCAA 51 AACCACATTG GTCGACCAAC TGCTGCGCCA ATCCGGCACA TTCCGCGCCA 101 ACCAGCAGGT TGACGAGCGC GTGATGGACA GCAACGACCT TGAAAAAGAA 151 CGCGGCATCA CCATCCTCGC CAAAAACACC GCCATCGATT ACGAAGGCTA 201 CCACATCAAT ATCGTCGACA CGCCGGGACA CGCCGACTTC GGCGGCGAAG 251 TAGAGCGAGT TTTGGGGATG GTGGACTGCG TCGTCTTGTT GGTGGACGCG 301 CAGGAAGGCC CGATGCCGCA AACCCGTTTC GTGACCAAAA AAGCCTTGGC 351 TTTGGGGCTG AAACCGATTG TCGTCATCAA TAAAATCGAC AAACCGTCCG 401 CCCGTCCGAG CTGGGTCATC GACCAAACTT TCGAGCTGTT CGACAACTTG 451 GGCGCGACCG ACGAGCAGTT GGATTTCCCG ATTGTTTATG CTTCCGGTCT 501 GTCCGGTTTC GCCAAATTGG AAGAAACCGA CGAGAGCAAC GACATGCGTC 551 CGCTGTTCGA TACTATCTTA AAATATACGC CTGCACCGAG CGGCAGCGCG 601 GACGAAACGC TGCAACTGCA AATTTCCCAA CTCGACTACG ACAACTACAC 651 CGGCCGCCTC GGTATCGGTC GTATCTTGAA CGGACGTATC AAGCCCGGTC 701 AAGTTGTTGC CGTCATGAAC CACGATCAAC AAATCGCCCA AGGCCGCATC 751 AACCAGCTTT TGGGTTTCAA AGGTTTAGAA CGCGTGCCGC TTGAAGAAGC 801 CGAAGCCGGC GACATCGTGA TTATTTCCGG TATTGAAGAC ATCGGCATCG 851 GCGTAACCAT CACCGACAAA GACAACCCCA AAGGCCTGCC GATGTTGAGC 901 GTGGACGAAC CGACGCTGAC GATGGACTTT ATGGTCAACA CCAGCCCGTT 951 GGCAGGTACG GAAGGCAAAT TCGTAACCAG CCGCCAAATC CGCGACCGCC 1001 TGCAAAAAGA ATTGCTGACC AACGTCGCCC TGCGCGTGGA AGATACCGCC 1051 GATGCCGACG TGTTCCGCGT ATCCGGGCGC GGCGAGCTGC ACCTGACCAT 1101 TTTGCTGGAA AACATGCGCC GCGAAGGCTA CGAACTCGCC GTCGGCAAAC 1151 CGCGCGTCGT GTACCGCGAC ATCGACGGTC AAAAATGCGA ACCGTATGAA 1201 AACCTGACCG TGGACGTACC CGACGACAAC CAAGGCGCGG TAATGGAAGA 1251 ACTCGGCCGC CGCCGTGGCG AACTGACTAA TATGGAAAGC GACGGCAACG 1301 GACGCACCCG CCTCGAATAC CATATTCCAG CGCGCGGCTT GATCGGCTTC 1351 CAAGGCGAAT TTATGACCCT GACGCGCGGG GTCGGGCTGA TGAGCCACGT 1401 GTTCGACGAT TACGCGCCCG TCAAACCCGA TATGCCTGGC CGCCACAACG 1451 GCGTGCTGGT GTCCCAAGAG CAGGGCGAGG CAGTCGCTTA CGCCTTGTGG 1501 AATCTGGAAG ACCGCGGCCG TATGTTCGTA TCGCCCAACG ACAAAATCTA 1551 CGAAGGTATG ATTATCGGCA TCCACAGTCG CGACAACGAT TTGGTGGTCA 1601 ACCCGCTCAA AGGCAAAAAA CTTACCAACA TCCGTGCCAG CGGTACCGAC 1651 GAAGCCGTTC GCCTGACCAC GCCGATTAAG CTGACGCTGG AAGGTGCGGT 1701 CGAGTTTATC GACGATGATG AGCTGGTAGA AATCACGCCG CAATCCATCC 1751 GTCTGCGCAA GCGTTACTTG AGCGAATTGG AACGCCGCCG CCATTTCAAA 1801 AAGCTAGATT GA This corresponds to the amino acid sequence <SEQ ID 618; ORF 151.a>: alδl.pep 1 MKQIRNIAII AHVDHGKTTL VDQLLRQSGT FRANQQVDER VMDSNDLEKE 51 RGITILAKNT AIDYEGYHIN IVDTPGHADF GGEVERVLGM VDCWLLVDA 101 QEGPMPQTRF VTKKALALGL KPIWINKID KPSARPSWVI DQTFELFDNL 151 GATDEQLDFP IVYASGLSGF AKLEETDESN DMRPLFDTIL KYTPAPSGSA 201 DETLQLQISQ LDYDNYTGRL GIGRILNGRI KPGQWAVMN HDQQIAQGRI 251 NQLLGFKGLE RVPLEEAEAG DIVIISGIED IGIGVTITDK DNPKGLPMLS 301 VDEPTLTMDF MVNTSPLAGT EGKFVTSRQI RDRLQKELLT NVALRVEDTA 351 DADVFRVSGR GELHLTILLE NMRREGYELA VGKPRWYRD IDGQKCEPYE 401 NLTVDVPDDN QGAVMEELGR RRGELTNMES DGNGRTRLEY HIPARGLIGF 451 QGEFMTLTRG VGLMSHVFDD YAPVKPDMPG RHNGVLVSQE QGEAVAYALW 501 NLEDRGRMFV SPNDKIYEGM IIGIHSRDND LWNPLKGKK LTNIRASGTD 551 EAVRLTTPIK LTLEGAVEFI DDDELVEITP QSIRLRKRYL SELERRRHFK 601 KLD* ml 51/al 51 99.8% identity in 603 aa overlap 10 20 30 40 50 60 l51.pep MKQIRNIAIIAHVDHGKTTLVDQLLRQΞGTFRANQQVDERVMDSNDLEKERGITILAKNT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a151 MKQI NI lIAHVDHGKTTLVDQLLRQΞGTFRANQQVDERVMDSNDLEKERGITI AKNT 10 20 30 40 50 60 70 80 90 100 110 120 l51.pep AIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQEGPMPQTRFVTKKALALGL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I al51 AIDYEGYHINIVDTPGHADFGGEVERVLGMVDCWLLVDAQEGPMPQTRFVTKKALALGL 70 80 90 100 110 120 130 140 150 160 170 180 ml51. .pep KPIWINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFPIVYASGLSGFAKLEETDESN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al51 KPIWINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFPIVYASGLSGFAKLEETDESN 130 140 150 160 170 180 190 200 210 220 230 240 ml51 .pep DMRPLFDTILKYTPAPSGSADETLQLQISQLDYDNYTGRLGIGRILNGRIKPGQTVAVMN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : | I I I I al51 DMRPLFDTILKYTPAPSGSADETLQLQISQLDYDNYTGRLGIGRILNGRIKPGQWAVMN 190 200 210 220 230 240 250 260 270 280 290 300 ml51. .pep HDQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIISGIEDIGIGVTITDKDNPKGLPMLS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al51 HDQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIISGIEDIGIGVTITDKDNPKGLPMLS 250 260 270 280 290 300 310 320 330 340 350 360 ml51.pep VDEPTLTMDFMVNTΞPLAGTEGKFVTSRQIRDRLQKELLTNVALRVEDTADADVFRVSGR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al51 VDEPTLTMDFMVNTSPLAGTEGKFVTΞRQIRDRLQKELLTNVALRVEDTADADVFRVSGR 310 320 330 340 350 360 370 380 390 400 410 420 ml 51 . pep GELHLTILLENMRREGYELAVGKPRWYRDIDGQKCEPYENLTVDVPDDNQGAVMEELGR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al51 GELHLTILLENMRREGYELAVGKPRWYRDIDGQKCEPYENLTVDVPDDNQGAVMEELGR 370 380 390 400 410 420 430 440 450 460 470 480 It l l51 . pep RRGELTNMEΞDGNGRTRLEYHIPARGLIGFQGEFMTLTRGVGLMSHVFDDYAPVKPDMPG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al51 RRGELTNMESDGNGRTRLEYHIPARGLIGFQGEFMTLTRGVGLMSHVFDDYAPVKPDMPG 430 440 450 460 470 480 490 500 510 520 530 540 ml51.pep RHNGVLVSQEQGEAVAYALWNLEDRGRMFVSPNDKIYEGMIIGIHSRDNDLWNPLKGKK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al51 RHNGVLVΞQEQGEAVAYALWNLEDRGRMFVΞPNDKIYEGMIIGIHSRDNDLWNPLKGKK 490 500 510 520 530 540 550 560 570 580 590 600 Itll51.pep LTNIRASGTDEAVRLTTPIKLTLEGAVEFIDDDELVEITPQΞIRLRKRYLSELERRRHFK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al51 LTNIRASGTDEAVRLTTPIKLTLEGAVEFIDDDELVEITPQSIRLRKRYLΞELERRRHFK 550 560 570 580 590 600 ml51.pep KLDX I I I I al51 KLDX The following partial DNA sequence was identified in N.gonorrhoeae <SEQ ID 619>: gl52.seq 1 ATGAAAAaca aAACCaaagt ctgGGacttc cCcacccgcc ttTTCCactG 51 GctgcttgCC gCATCCctgc CCTTTATGTG gtatagCGCA AAAGCCGGCG 101 GcgataTGCT GcaatgGCAC ACGCGCGTCG GGCTGCTCGT CCTTTTCCTG 151 CTCGTATTCC GCCTCTGCTG GGGCATTTGG GGCAgcgATA CCGCCCGTTT 201 CTCccgTtTC GTCCGAGGTT GGGCAGGTAT ACGCGGCTAT CTGAAAAAcg 251 gCATTCCCGA ACAtatcCAG CCCGGACACA ACCCCTTGGG CGCACTgatg 301 gtcGTTGCGC TTTTGgccgc cgtcTCATTT CAagtcggcA CGGGGCTTTT 351 Tgccgccaat gaaaacacct tcagcaCCAa cggctacctc aaccatttgg 401 tttccgaaca tacgGGCAGC CTTATACGGA AAATCCACCT CAACTTTTTC 451 AAGCTGCTCG CCGTTTTTTC CGCAGTCCAC ATCGCCGCCG TCGCCGCATA 501 CCGCATATTC AAAAAGAAAA ACCTCGTCCG CCCGATGATA ACCGGCTTCA 551 AATACATCGA AGGCAAAACC TCAATCCGCT TTGCCGGCAA AGCCGCGCTT 601 GCCGCCGCAT TATCGGTTGC CGCGCTTGCC GCAGCCGCCA TCCTGCTCCT 651 GTCCTGA This corresponds to the amino acid sequence <SEQ ID 620; ORF 152.ng>: gl52.pep 1 MKNKTKVWDF PTRLFHWLLA ASLPFMWYSA KAGGDMLQWH TRVGLLVLFL 51 LVFRLCWGIW GSDTARFSRF VRGWAGIRGY LKNGIPEHIQ PGHNPLGALM 101 WALLAAVSF QVGTGLFAAN ENTFSTNGYL NHLVSEHTGS LIRKIHLNFF 151 KLLAVFSAVH IAAVAAYRIF KKKNLVRPMI TGFKYIEGKT SIRFAGKAAL 201 AAALSVAALA AAAILLLS* The following partial DNA sequence was identified in N. meningitidis <SEQ LD 621>: ml52. seq 1 ATGAAAAACA AAACCAAAGT CTGGGACCTC CCCACCCGCC TTTTCCACTG 51 GCTGCTTGCC GCGTCCCTGC CCTTTATGTG GTATAGCGCG AAAGCCGGCG 101 GCGATATGCT GCAATGGCAC ACGCGCGTCG GGCTGTTCGT CCTTTTCCTG 151 CTCGTATTTC GCCTCTGCTG GGGCATTTGG GGCAGCGATA CCGCCCGTTT 201 TTCCCGTTTC GTCCAAGGCT GGGCAGGCAT ACGCGGCTAT CTGAAAAACG 251 GTATTCCCGA ACACATCCAG CCCGGACACA ACCCCTTGGG CGCACTGATG 301 GTCGTTGCGC TTTTGGCCGC CGTGTCCTTC CAAGTCGGCA CCGGGCTTTT 351 TGCCGCCGAT GAAAACACCT TCAGCACCAA CGGCTACCTC AACCATTTGG 401 TTTCCGAACA TACGGGCAGC CTTATGCGGA AAATCCACCT CAACTTTTTC 451 AAGCTGCTCG CCGTTTTTTC TGCAATCCAC ATCGCCGCCG TCGCCGCATA 501 CCGCGTATTC AAAAAGAAAA ACCTCATCCT CCCGATGATA ACCGGCTTCA 551 AATACATCGA AGGCAAAACC TCAATCCGCT TTGCAGGCAA AGCCGCGCTT 601 GCCGCCGCAT TATCGGTTGC CTCGCTTGCC GCAGCCGCCA TCCTGCTCCT 651 GTCCTGA This corresponds to the amino acid sequence <SEQ ID 622; ORF 152>: ml52. pep 1 MKNKTKVWDL PTRLFHWLLA ASLPFMWYSA KAGGDMLQWH TRVGLFVLFL 51 LVFRLCWGIW GSDTARFSRF VQGWAGIRGY LKNGIPEHIQ PGHNPLGALM 101 WALLAAVSF QVGTGLFAAD ENTFSTNGYL NHLVSEHTGS LMRKIHLNFF 151 KLLAVFSAIH IAAVAAYRVF KKKNLILPMI TGFKYIEGKT SIRFAGKAAL 201 AAALSVASLA AAAILLLS* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 152 shows 95.4% identity over a 218 aa overlap with a predicted ORF (ORF 152.ng) from N gonorrhoeae: ml52/gl52 10 20 30 40 50 60 ml52.pep MKΝKTKVWDLPTRLFHWLLAASLPFMWYSAKAGGDMLQWHTRVGLFVLFLLVFRLCWGIW gl52 MKΝKTKVWDFPTRLFHWLLAASLPFMWYSAKAGGDMLQWHTRVGLLVLFLLVFRLCWGIW 10 20 30 40 50 60 70 80 90 100 110 120 ml52.pep GSDTARFSRFVQGWAGIRGYLKΝGIPEHIQPGHΝPLGALMWALLAAVSFQVGTGLFAAD gl52 GSDTARFSRFVRGWAGIRGYLKΝGIPEHIQPGHΝPLGALMWALLAAVSFQVGTGLFAAΝ 70 80 90 100 110 120 130 140 150 160 170 180 ml52. pep ENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAIHIAAVAAYRVFKKKNLILPMI III gl52 ENTFSTNGYLNHLVSEHTGSLIRKIHLNFFKLLAVFSAVHIAAVAAYRIFKKKNLVRPMI 130 140 150 160 170 180 190 200 210 219 ml52.pep TGFKYIEGKTSIRFAGKAALAAALSVASLAAAAILLLSX gl52 TGFKYIEGKTSIRFAGKAALAAALSVAALAAAAILLLSX 190 200 210 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 623>: al52. seq 1 ATGAAAAACA AAACCAAAGT CTGGGACTTC CCCACCCGCC TTTTCCACTG 51 GCTGCTTGCC GCATCCCTAC CCTTTATGTG GTATAGCGCG AAAACCGGCG 101 GCGATATGCT GCAATGGCAC ACGCGCGTCG GGCTGTTTAT CCTTTTCCTG 151 CTCGTATTCC GCCTCTGCTG GGGCATTTGG GGCAGCGATA CCGCCCGTTT 201 CTCCCGTTTC GTCCGCGGAT GGTCGGGTAT CAGAGAGTAT ATGAAAAACG 251 GTATTCCCGA ACACGTCCAA CCCGGACACA ACCCCTTGGG CGCACTGATG 301 GTCGTTGCGC TTTTGGCCGC CGTGTCGTTC CAAGTCGGCA CAGGGCTTTT 351 TGCCGCCGAT GTAAACACCT TCAGCACCAA CGGCTACCTC AACCATTTGG 401 TTTCCGAACA TACGGGCAGC CTTATGCGGA AAATCCATCT CAACTTTTTC 451 AAACTGCTCG CCGTTTTTTC CGCAGTCCAC ATCGCCGΝCG TCGCCGCATA 501 CCGCGTGTTC AAAAAGAAAA ACCTCGTCCT CCCGATGATA ACCGGCTTCA 551 AATACATCGA AGGCAAAACC TCAATCCGCT TTGCCGGCAA AGCCGCGCTT 601 GCCGCCGCAT TATCGGTTGC CGCGCTTGCC GCAGCCGCCA TCCTGCTCCT 651 GTCCTGA This corresponds to the amino acid sequence <SEQ ID 624; ORF 152.a>: al52.pep 1 MKΝKTKVWDF PTRLFHWLLA ASLPFMWYSA KTGGDMLQWH TRVGLFILFL 51 LVFRLCWGIW GSDTARFSRF VRGWSGIREY MKΝGIPEHVQ PGHNPLGALM 101 WALLAAVSF QVGTGLFAAD VNTFSTNGYL NHLVSEHTGS LMRKIHLNFF 151 KLLAVFSAVH IAXVAAYRVF KKKNLVLPMI TGFKYIEGKT SIRFAGKAAL 201 AAALSVAALA AAAILLLS* ml52/al52 94.0% identity in 218 aa overlap 10 20 30 40 50 60 ml52. pep MKNKTKVWDLPTRLFHWLLAASLPFMWYSAKAGGDMLQWHTRVGLFVLFLLVFRLCWGIW I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I : I I I I I I I I I I I I I al52 MKNKTKVWDFPTRLFHWLLAASLPFMWYSAKTGGDMLQWHTRVGLFILFLLVFRLCWGIW 10 20 30 40 50 60 70 80 90 100 110 120 ml52. pep GSDTARFSRFVQGWAGIRGYLKNGIPEHIQPGHNPLGALMWALLAAVSFQVGTGLFAAD I I I I I I I I I I I : I I : II I I : I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al52 GSDTARFSRFVRGWSGIREYMKNGIPEHVQPGHNPLGALMWALLAAVSFQVGTGLFAAD 70 80 90 100 110 120 130 140 150 160 170 180 ml52. pep ENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAIHIAAVAAYRVFKKKNLILPMI I II I I I I I I I I I I I I II I II I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I : I I I I al52 VNTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAVHIAXVAAYRVFKKKNLVLPMI 130 140 150 160 170 180 190 200 210 219 ml52.pep TGFKYIEGKTSIRFAGKAALAAALSVASLAAAAILLLSX I I I I I I I I I I I I II I I I I I I I I I I I I I : I I I I I I I I I I I al52 TGFKYIEGKTSIRFAGKAALAAALSVAALAAAAILLLSX 190 200 210 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 625>: gl53.seq 1 atggggtttg cttaCAgtat gacgtatatc gaggtCGGGa taccggaggc 51 ggcatccgtc ctttCgctGC CCGAGATgat gcgcctgatG GTGTTtCagg 101 attATGGTTT TttggcCGAA GTGATGTTTG TGctgaCTTT cGGCGcgcCG 151 GTTCTGTTtC TGCTGCTGTG CCTGTATGTC TATGCCGCGC TGATACGGAA 201 ACAGGCGTAT CCTGCGCTGC GTTTGGCAAC GCGTGTGATG GTGCGCTTGA 251 GGCAGGCGAT GATGGTGGAT GTGTTTTTTG TTTCCACTCT GGTGGCGTAT 301 ATCAAGCTCT CGTCTGTGGC AAAGGTTCGC TTCGGGCCGG CGTTTTATCT 351 GATGTTCGCG CTGTCGGTTA TGCTGATTCG GACTTCGGTA TCGGTTCCCC 401 AGCATTGGGT GTATTTCCAA ATCGGGCGGC TGACGGGGAA TAATGCGGTT 451 CAGACGGCAT CGGAAGGCAA AACCTGTTGC AGCCGCTGCC TGTATTTccg 501 cgacAGTgcc gaatccCCCT GCGGGGTGTg cgGCGcggaA CTgtacggcg 551 gacggccgaa aagtCTGAGt atttCgtCGG CGTTTCTgac ggcggcggTT 601 GTTTTGTATT TCCctgCcaa TATCctgccg attaTGAttt cgtccAATCc 651 tgccgccacg GAGGcCAACA CCATCTTTAG CGGCATCGCT TATATGTGGG 701 ACGagggcgA CAGGCTGATT GCGGCGGTTA TTTTCAGCGC GAGTATTTTG 751 GTGCCGGTGC TGAAGATTGC GGCAATGTCG GTTTTGATTG CGGCGGCACG 801 GTTCGCTTTG CCGGCGGGCG CAAAGAAATT GTCGCACCTC tacCGCATCA 851 CCGAAGCGGT CGGCCGCTGG TCGATGATTG ATATTTTTGT GATTATTATT 901 TTGATGTGTT CGTTCCacaC TTATGCCGCG CGCGTCATTC CGGGCAGTGC 951 GGCAGTCTAT TTCTGCCTGG TCGTGATTTT GACGATGCTG TCCGCCTATT 1001 ATTTCGACCC GCGCCTGCTT TGGGACAAAC GCGCTTCAGA CGGCATTGCT 1051 TTCAACGAAA CGGAAAAATA TGACTGA This corresponds to the amino acid sequence <SEQ ID 626; ORF 153.ng>: gl53.pep 1 MGFAYSMTYI EVGIPEAASV LSLPEMMRLM VFQDYGFLAE VMFVLTFGAP 51 VLFLLLCLYV YAALIRKQAY PALRLATRVM VRLRQAMMVD VFFVSTLVAY 101 IKLSSVAKVR FGPAFYLMFA LSVMLIRTSV SVPQHWVYFQ IGRLTGΝΝAV 151 QTASEGKTCC SRCLYFRDSA ESPCGVCGAE LYGGRPKSLS ISSAFLTAAV 201 VLYFPAΝILP IMISSΝPAAT EAΝTIFSGIA YMWDEGDRLI AAVIFSASI 251 VPVLKIAAMS VLIAAARFAL PAGAKKLSHL YRITEAVGRW SMIDIFVIII 301 LMCSFHTYAA RVIPGSAAVY FCLWILTML SAYYFDPRLL WDKRASDGIA 351 FΝETEKYD* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 627>: ml53.seq 1 ATGGCGTTTG CTTACGGTAT GACGTATATC GAGGTCGGGA TACCGGGTGC 51 GGCATCCGTC CTTTCGCTGC CCGAGATGAT GCGCCTGATG GTGTTTCAGG 101 ATTATGGTTT TTTGGCCGAA GTGATGTTTG TGCTGACTTT CGGCGCGCCG 151 GTTCTGTTTC TGCTGCTGTG CCTGTATGTC TATGCCGCGC TGATACGGAA 201 ACAGGCGTAT CCTGCGCTGC GTTTGGCAAC GCGTGTGATG GTGCGCTTGA 251 GACAGGCGAT GATGGTGGAT GTGTTTTTTG TTTCCACTTT GGTGGCGTAT 301 ATCAAGCTCT CGTCTGTGGC AGAGGTTCGC TTCGGGCCGG CGTTTTATCT 351 GATGTTCGCG CTGTCAGTTA TGCTGATTCG GACTTCGGTA TCGGTTCCCC 401 AGCATTGGGT GTATTTTCAA ATCGGGCGGC TGACGGGGGA TAATGCGGTT 451 CAGACGGCAT CGGAAGGTAA AACCTGTTGC AGCCGCTGCC TGTATTTCCG 501 CGACAGTGCC GAATCCCCCT GCGGCGTGTG CGGTGCGGAA CTGTACCGCC 551 GACGGCCGAA AAGTCTGAGT ATTTCGTCGG CGTTTCTGAC GGCGGCGGTT 601 ATTTTGTATT TCCCTGCCAA TATCCTGCCG ATTATGATTT CGTCCAATCC 651 TGCCGCCACG GAGGTCAATA CCATCCTTAA CGGCATCGCT TATATGTGGG 701 ACGAGGGCGA CAGGCTGATT GCGGCGGTTA TTTTCAGCGC GAGTATTTTG 751 GTGCCGGTAC TGAAGATTGC GGCAATGTCG GTTTTGATTG CGTCCGCCCG 801 CTTCGCTTTG CCAACGGGTG CAAAGAAATT GTCGCACCTC TACCGCATCA 851 CCGAAGCGGT CGGCCGCTGG TCGATGATTG ATATTTTTGT GATTATTATT 901 TTGATGTGTT CGTTCCACAC TTATGCCGCG CGCGTCATTC CGGGCAGTGC 951 GGCAGTCTAT TTCTGCCTGG TCGTGATTCT GACGATGCTG TCCGCCTATT 1001 ATTTCGACCC GCGCCTGCTT TGGGACAAAC GCGCTTCAGA CGGCATTGCT 1051 TTCAATGAAA CGGAAAAACA TGACTGA This corresponds to the amino acid sequence <SEQ ID 628; ORF 153>: ml53.pep 1 MAFAYGMTYI EVGIPGAASV LSLPEMMRLM VFQDYGFLAE VMFVLTFGAP 51 VLFLLLCLYV YAALIRKQAY PALRLATRVM VRLRQAMMVD VFFVSTLVAY 101 IKLSSVAEVR FGPAFYLMFA LSVMLIRTSV SVPQHWVYFQ IGRLTGDNAV 151 QTASEGKTCC SRCLYFRDSA ESPCGVCGAE LYRRRPKSLS ISSAFLTAAV 201 ILYFPANILP IMISSNPAAT EVNTILNGIA YMWDEGDRLI AAVIFSASIL 251 VPVLKIAAMS V IASARFAL PTGAKKLSHL YRITEAVGRW SMIDIFVIII 301 LMCSFHTYAA RVIPGSAAVY FCLWILTML SAYYFDPRLL WDKRASDGIA 351 FNETEKHD* ml53 / gl53 96.1% identity in 358 aa overlap 10 20 30 40 50 60 ml53.pep MAFAYGMTYIEVGIPGAASVLSLPEMMRLMVFQDYGFLAEVMFVLTFGAPVLFLLLCLYV gl53 MGFAYSMTYIEVGIPEAASVLSLPEMMRLMVFQDYGFLAEVMFVLTFGAPVLFLLLCLYV 10 20 30 40 50 60 70 80 90 100 110 120 ml53.pep YAALIRKQAYPALRLATRVMVRLRQAMMVDVFFVSTLVAYIKLSSVAEVRFGPAFYLMFA gl53 YAALIRKQAYPALRLATRVMVRLRQAMMVDVFFVSTLVAYIKLSSVAKVRFGPAFYLMFA 70 80 90 100 110 120 130 140 150 160 170 180 ml53.pep LΞVMLIRTSVSVPQHWVYFQIGRLTGDNAVQTASEGKTCCSRCLYFRDSAESPCGVCGAE 111111 II 111111111111111111 • • I II 11 II 11111111111 II 111 II 1111 II 11 gl53 LSVMLIRTSVSVPQHWVYFQIGRLTGNNAVQTASEGKTCCSRCLYFRDSAESPCGVCGAE 130 140 150 160 170 180 190 200 210 220 230 240 ml53.pep LYRRRPKSLSISSAFLTAAVILYFPANILPIMISSNPAATEVNTILNGIAYMWDEGDRLI I I gl53 LYGGRPKSLSISSAFLTAAWLYFPANILPIMISSNPAATEANTIFSGIAYMWDEGDRLI 190 200 210 220 230 240 250 260 270 280 290 300 ml53 . ep AAVIFSASILVPVLKIAAMSVLIASARFALPTGAKKLΞHLYRITEAVGRWSMIDIFVIII gl53 AAVIFSASILVPVLKIAAMSVLIAAARFALPAGAKKLSHLYRITEAVGRWSMIDIFVIII 250 260 270 280 290 300 310 320 330 340 350 359 ml53 . pep LMCSFHTYAARVIPGSAAVYFCLWILTMLSAYYFDPRLLWDKRASDGIAFNETEKHDX gl53 LMCSFHTYAARVIPGSAAVYFCLWILTMLSAYYFDPRLLWDKRAΞDGIAFNETEKYDX 310 320 330 340 350 The following partial DNA sequence was identified in N meningitidis <SEQ ID 629>: al53.seq 1 ATGGCGTTTG CTTACGGTAT GACGTATATC GAGGTCGGGA TACCGGGTGC 51 GGCATCCGTC CTTTCGCTGC CCGAGATGAT GCGCCTGATG GTGTTTCAGG 101 ATTATGGTTT TTTGGCCGAA GTGATGTTTG TGCTGACCTT CGGCGCGCCG 151 GTTCTGTTTC TGCTGCTGTG CCTGTATGTC TATGCCGCGC TGATACGGAA 201 ACAGGCGTAT CCTGCGCTGC GTTTGGCAAC GCGTGTGATG GTGCGCTTGA 251 GACAGGCGAT GATGGTGGAT GTGTTTTTTG TTTCCACTTT GGTGGCGTAT 301 ATCAAGCTCT CGTCTGTGGC AGAGGTTCGC TTCGGATCGG CGTTTTATCT 351 GATGTTCGCG CTGTCGGTTA TGCTGATTCG GACTTCGGTA TCGGTTCCCC 401 AGCATTGGGT GTATTTTCAA ATCGGGCGGC TGACGGGGGA TAATGCGGTT 451 CAGACGGCAT CGGAAGGTAA AACCTGTTGC AGCCGCTGCC TGTATTTCCG 501 CGACAGTGCC GAATCCCCCT GCGGCGTGTG CGGTGCGGAA CTGTACCGCC 551 GACGGCCGAA AAGTCTGAGT ATTTCGTCGG CGTTTCTGAC GGCGGCGGTT 601 ATTTTGTATT TCCCTGCCAA TATCCTGCCG ATTATGATTT CGTCCAATCC 651 TGCCGCCACG GAGGTCAATA CCATCCTTAA CGGCATCGCT TATATGTGGG 701 ACGAGGGCGA CAGGCTGATT GCGGCGGTTA TTTTCAGCGC GAGTATTTTG 751 GTGCCGGTAC TGAAGATTGC GGCAATGTCG GTTTTGATTG CGTCCGCCCG 801 CTTCGCTTTG CCAACGGGTG CAAAGAAATT GTCGCACCTC TACCGCATCA 851 CCGAAGCGGT CGGCCGCTGG TCGATGATTG ATATTTTTGT GATTATTATT 901 TTGATGTGTT CGTTCCACAC TTATGCCGCG CGCGTCATTC CGGGCAGTGC 951 GGCAGTCTAT TTCTGCCTGG TCGTGATTCT GACGATGCTG TCCGCCTATT 1001 ATTTCGACCC GCGCCTGCTT TGGGACAAAC GCGCTTCAGA CGGCATTGCT 1051 TTCAATGAAA CGGAAAAACA TGACTGA This corresponds to the amino acid sequence <SEQ ID 630; ORF 153.a>: al53.pep 1 MAFAYGMTYI EVGIPGAASV LSLPEMMRLM VFQDYGFLAE VMFVLTFGAP 51 VLFLLLCLYV YAALIRKQAY PALRLATRVM VRLRQAMMVD VFFVSTLVAY 101 IKLSSVAEVR FGSAFYLMFA LSVMLIRTSV SVPQHWVYFQ IGRLTGDNAV 151 QTASEGKTCC SRCLYFRDSA ESPCGVCGAE LYRRRPKSLS ISSAFLTAAV 201 ILYFPANILP IMISSNPAAT EVNTILNGIA YMWDEGDRLI AAVIFSASIL 251 VPVLKIAAMS VLIASARFAL PTGAKKLSHL YRITEAVGRW SMIDIFVIII 301 LMCSFHTYAA RVIPGSAAVY FCLWILTML SAYYFDPRLL WDKRASDGIA 351 FNETEKHD* ml 53/al 53 99.7% identity in 358 aa overlap 10 20 30 40 50 60 ml53. pep MAFAYGMTYIEVGIPGAASVLSLPEMMRLMVFQDYGFLAEVMFVLTFGAPVLFLLLCLYV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al53 MAFAYGMTYIEVGIPGAASVLSLPEMMRLMVFQDYGFLAEVMFVLTFGAPVLFLLLCLYV 10 20 30 40 50 60 70 80 90 100 110 120 ml53. pep YAALIRKQAYPALRLATRVMVRLRQAMMVDVFFVSTLVAYIKLSSVAEVRFGPAFYLMFA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I lllllll al53 YAALIRKQAYPALRLATRVMVRLRQAMMVDVFFVSTLVAYIKLSSVAEVRFGSAFYLMFA 70 80 90 100 110 120 130 140 150 160 170 180 ml53.pep LSVMLIRTSVSVPQHWVYFQIGRLTGDNAVQTASEGKTCCSRCLYFRDSAESPCGVCGAE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I II I I I I I I I I I I I al53 LSVMLIRTSVSVPQHWVYFQIGRLTGDNAVQTASEGKTCCSRCLYFRDSAESPCGVCGAE 130 140 150 160 170 180 190 200 210 220 230 240 ml53. pep LYRRRPKSLSISSAFLTAAVILYFPANILPIMISΞNPAATEVNTILNGIAYMWDEGDRLI I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I al53 LYRRRPKSLSISSAFLTAAVILYFPANILPIMISSNPAATEVNTILNGIAYMWDEGDRLI 190 200 210 220 230 240 250 260 270 280 290 300 ml53.pep AAVIFSASILVPVLKIAAMSVLIASARFALPTGAKKLSHLYRITEAVGRWSMIDIFVIII I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I al53 AAVIFSASILVPVLKIAAMSVLIASARFALPTGAKKLSHLYRITEAVGRWSMIDIFVIII 250 260 270 280 290 300 310 320 330 340 350 359 ml53. pep LMCSFHTYAARVIPGSAAVYFCLVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKHDX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al53 LMCSFHTYAARVIPGSAAVYFCLVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKHDX 310 320 330 340 350 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 631>: gl54.seq 1 ATGACTGACA ACAGCCCTCC TCCAAACGGA CACGCTCAAG CACGCGTCCG 51 CAAAAACAAC accttCCtCT CCGCCGTCTG GCTGGTCCCG CTGATCGCGC 101 TGATTGCCGG CGGCTGGCTT TGGGTTAAGG AAATCCGCAA CAGGGGGCCT 151 GTGGTTACGC TCTTGATGGA CAGCGCGGAA GGCATCGAAG TCAACAATAC 201 GGTCATTAAG GTATTGAGCA TCGATGTCGG ACGCGTTACC CGAATCAAAC 251 TGCGCGACGA CCAAAAAGGC GTGGAAGTTA CTGCCCAACT CAATGCGGAC 301 GTATCCGGCC TCATCCGCAG CGATACCCAG TTTTGGGTGG TCAAGCCGCG 351 TATCGACCAA AGCGGcgtAA CCGGTTTGGG TACGCTGCTT TCGGGTTCGT 401 ACATCGCTTT TACACCCGGC AAAAGCGGCG AGGCAAAAGA CGTGTTCCAA 451 GTGCAGGACA TTCCGCCCGT TACCGCCATC GGGCAAAgcg GGCTGCGCTT 501 GAATTTGATT GGTAAAAACG AccgCATCCT CAACGTcaaC AGCCCTGTTT 551 TGTATGAAAA CTTTATGGTC GGGCAAATCG AAAGCGCGCA TTTCGAcccG 601 TCCGACCAAA GCGTGCATTA CACCATCTTC ATCCAAAGCC CCAACGACAA 651 ACTGATTCAT TCCGCCAGCC GTTTTTGGCT GGAAAGCGGC ATCAATATCG 701 AAACCACAGG CAGCGGCATC AAACTCAATT CCGCCCCTCT GCCTGCCCTG 751 CTGTCAGGCG CGATTTCATT TGATTCGCCG AAAACCAAAA ACAGTAAAAA 801 CGTCAAAAGC GAGGACAGCT TCACGCTTTA CGACAGCCGC AGCGAAATCG 851 CCAACCTGCC TGACGACCGC TCGCTGTACT ACACCGCGTT TTTCAAACAA 901 TCCGTGCGCG GACTGACCGT cggTTCGCCT GTcgaATACA AAGGGCtgaA 951 TGTcggCATG GTTTCCGATG TCCCTTATTT TGACCGCAAt gacagCCTGC 1001 ACCtgtTTGA aaacggctgg aTTcccGtac gCATCCGCAT cgagccTTCC 1051 CGTTTGGAAA TCAATGCCGA CGAGCAAAGC AAAGAGCATT GGAAACAACA 1101 ATTCCAGACG GCCTTAAACA AAGGCCTGAC CGCCACCATC TCCAGCAACA 1151 ACCTGCTGAC CGGCGGCAAA ATGATTGAGT TGAACGATCA GCCTTCCGCC 1201 TCGCCCAAGC TGCGACCGCA TACCGTTTAT GCAGGCGATA CCGTCATCGC 1251 CACACGGGGC GGCGGTTTGG ATGACTTGCA GGTCAAATTG GCGGATTTGC 1301 TGGACaaatT CAACAATCTG CCATTggata aAACCGTTGC CGAATTGAAC 1351 GGCTCGCTCG CCGAACTCAA GTCCGCACTC AAATCCGCCA ATGCCGCCCT 1401 AAGCTCCATT GacaAACTGG TCGgcaaTCC GCAGACGCAA AACATCCCGA 1451 ACGAACTGAA CCAAACTCTG AAAGAGTTGC GCATAACCCT GCAAGGCGTA 1501 TCGcctCAAT CGCCTATCTa cgGAgacgta caAAATAcgc tgCaAAGTTT 1551 GGACAAAACC TTAAAagacg TtcaACCCGT CATTAACACT TTGAaAGAAa 1601 aacCCaaCgc actGATTTtc aacaACAGCA GCAAAGAccc tATCCCGAAA 1651 GGAAGCCGAT AA This corresponds to the amino acid sequence <SEQ LD 632; ORF 154.ng>: gl54.pep 1 MTDNSPPPNG HAQARVRKNN TFLSAVWLVP LIALIAGGWL WVKEIRNRGP 51 WTLLMDSAE GIEVNNTVIK VLSIDVGRVT RIKLRDDQKG VEVTAQLNAD 101 VSGLIRSDTQ FWWKPRIDQ SGVTGLGTLL SGSYIAFTPG KSGEAKDVFQ 151 VQDIPPVTAI GQSGLRLNLI GKNDRILNVN SPVLYENFMV GQIESAHFDP 201 SDQSVHYTIF IQSPNDKLIH SASRFWLESG INIETTGSGI KLNSAPLPAL 251 LSGAISFDSP KTKNΞKNVKS EDSFTLYDSR SEIANLPDDR SLYYTAFFKQ 301 SVRGLTVGSP VEYKGLNVGM VSDVPYFDRN DSLHLFENGW IPVRIRIEPS 351 RLEINADEQS KEHWKQQFQT ALNKGLTATI SSNNLLTGGK MIELNDQPSA 401 SPKLRPHTVY AGDTVIATRG GGLDDLQVKL ADLLDKFNNL PLDKTVAELN 451 GSLAELKSAL KSANAALΞΞI DKLVGNPQTQ NIPNELNQTL KELRITLQGV 501 SPQSPIYGDV QNTLQSLDKT LKDVQPVINT LKEKPNALIF NNSSKDPIPK 551 GSR* The following partial DNA sequence was identified in N meningitidis <SEQ TD 633>: ml54.seq 1 ATGACTGACA ACAGCCCTCC TCCAAACGGA CACGCCCAAG CACGCGTCCG 51 CAAAAACAAC ACCTTCCTCT CTGCCGTCTG GCTGGTTCCG CTGATCGCGC 101 TGATTGCCGG CGGCTGGCTT TGGGTTAAGG AAATCCGCAA CAGGGGGCCT 151 GTGGTTACGC TCTTGATGGA CAGCGCGGAA GGCATTGAGG TCAACAATAC 201 GGTCATCAAA GTATTGAGCA TCGATGTCGG ACGCGTTACC CGAATCAAAC 251 TGCGCGACGA CCAAAAAGGC GTGGAAGTAA CCGCCCAACT CAATGCGGAC 301 GTATCCGGCC TCATCCGCAG CGATACCCAG TTTTGGGTGG TCAAGCCGCG 351 TATCGACCAA AGCGGCGTAA CCGGTTTGGG TACGCTGCTT TCGGGTTCGT 401 ACATCGCCTT TACACCCGGC AAAAGCGACG AGGCAAAAGA CGTGTTCCAA 451 GTGCAGGACA TTCCGCCCGT TACCGCCATC GGGCAAAGCG GGCTGCGCTT 501 GAATTTGATT GGTAAAAACG ACCGCATCCT CAACGTCAAC AGCCCTGTTT 551 TGTATGAAAA TTTTATGGTC GGGCAAGTCG AAAGCGCGCA TTTCGACCCG 601 TCCGACCAAA GCGTGCATTA CACCATCTTC ATCCAAAGCC CCAACGACAA 651 ACTGATTCAT TCCGCCAGCC GTTTTTGGCT GGAAAGCGGC ATCAATATCG 701 AAACCACAGG CAGCGGCATC AAACTCAATT CCGCCCCTCT GCCTGCCCTG 751 CTGTCGGGCG CGATTTCATT TGATTCGCCG AAAACCAAAA ACAGTAAAAA 801 CGTCAAAAGC GAAGACAGCT TCACGCTTTA CGACAGCCGC AGCGAAGTCG 851 CCAACCTGCC TGACGACCGC TCGCTGTACT ACACCGCGTT TTTCAAACAA 901 TCCGTGCGCG GCCTGACCGT CGGTTCGCCC GTCGAGTACA AAGGGCTGAA 951 TGTCGGCGTG GTTTCCGACG TTCCTTATTT CGACCGCAAC GACAGCCTGC 1001 ACCTGTTTGA AAACGGCTGG ATACCCGTAC GCATCCGCAT TGAACCTTCC 1051 CGTTTGGAAA TCAATGCCGA CGAACAAAGC AAAGAACATT GGAAACAACA 1101 ATTTCAGACG GCCTTAAACA AAGGCCTGAC CGCCACCATC TCCAGCAACA 1151 ACCTGCTGAC CGGAAGCAAA ATGATTGAGT TGAACGATCA GCCTTCCGCA 1201 TCACCTAAGC TGCGACCGCA TACCGTTTAT GCAGGCGATA CCGTTATCGC 1251 GACCCAGGGC GGCGGTTTGG ACGATTTGCA GGTCAAATTG GCGGATTTGC 1301 TGGACAAGTT CGACAAACTG CCTTTAGATA AGACGGTTGC CGAATTGAAC 1351 GGTTCGCTTG CCGAGCTCAA ATCCACACTC AAATCTGCCA ATGCCGCCCT 1401 AAGCTCCATC GACAAACTGG TCGGCAAACC GCAGACACAA AACATTCCGA 1451 ACGAACTGAA CCAAACCCTG AAAGAGTTGC GCACAACCCT GCAAGGCGTA 1501 TCGCCGCAAT CGCCTATCTA CGGCGACGTA CAAAATACGC TGCAAAGTTT 1551 GGACAAAACT TTAAAAGACG TTCAACCCGT GATTAATACT TTGAAAGAAA 1601 AACCCAACGC GCTGATTTTC AACAGCAGCA GCAAAGACCC TATCCCGAAA 1651 GGAAGCCGAT AA This corresponds to the amino acid sequence <SEQ ID 634 ORF ml54.pep 1 MTDNSPPPNG HAQARVRKNN TFLSAVWLVP LIALIAGGWL WVKEIRNRGP 51 WTLLMDSAE GIEVNNTVIK VLSIDVGRVT RIKLRDDQKG VEVTAQLNAD 101 VSGLIRSDTQ FWWKPRIDQ SGVTGLGTLL SGSYIAFTPG KSDEAKDVFQ 151 VQDIPPVTAI GQSGLRLNLI GKNDRILNVN SPVLYENFMV GQVESAHFDP 201 SDQSVHYTIF IQSPNDKLIH SASRFWLESG INIETTGSGI KLNSAPLPAL 251 LSGAIΞFDSP KTKNSKNVKS EDSFTLYDSR SEVANLPDDR SLYYTAFFKQ 301 SVRGLTVGSP VEYKGLNVGV VSDVPYFDRN DSLHLFENGW IPVRIRIEPS 351 RLEINADEQS KEHWKQQFQT ALNKGLTATI SSNNLLTGSK MIELNDQPSA 401 SPKLRPHTVY AGDTVIATQG GGLDDLQVKL ADLLDKFDKL PLDKTVAELN 451 GSLAELKSTL KSANAALSSI DKLVGKPQTQ NIPNELNQTL KELRTTLQGV 501 SPQSPIYGDV QNTLQSLDKT LKDVQPVINT LKEKPNALIF NSSSKDPIPK 551 GSR* ml54 / gl54 97.8% identity in 553 aa overlap 10 20 30 40 50 60 ml54.pep MTDNSPPPNGHAQARVRKNNTFLSAVWLVPLIALIAGGWLWVKEIRNRGPWTLLMDSAE gl54 MTDNSPPPNGHAQARVRKNNTFLSAVWLVPLIALIAGGWLWVKEIRNRGPWTLLMDSAE 10 20 30 40 50 60 70 80 90 100 110 120 ml54.pep GIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVSGLIRSDTQFWWKPRIDQ gl54 GIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVSGLIRSDTQFWWKPRIDQ 70 80 90 100 110 120 130 140 150 160 170 180 ml54.pep SGVTGLGTLLSGΞYIAFTPGKSDEAKDVFQVQDIPPVTAIGQSGLRLNLIGKNDRILNVN I M I I I I I I I I M I I I I I M I I 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 I I 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 gl54 SGVTGLGTLLSGSYIAFTPGKSGEAKDVFQVQDIPPVTAIGQSGLRLNLIGKNDRILNVN 130 140 150 160 170 180 190 200 210 220 230 240 ml54.pep SPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKLIHSASRFWLESGINIETTGSGI gl54 SPVLYENFMVGQIESAHFDPSDQSVHYTIFIQSPNDKLIHSASRFWLESGINIETTGSGI 190 200 210 220 230 240 250 260 270 280 290 300 ml54.pep KLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTLYDSRSEVANLPDDRSLYYTAFFKQ gl54 KLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTLYDSRSEIANLPDDRSLYYTAFFKQ 250 260 270 280 290 300 310 320 330 340 350 360 l54 . pep SVRGLTVGSPVEYKGLNVGWSDVPYFDRNDSLHLFENGWIPVRIRIEPSRLEINADEQS gl54 SVRGLTVGSPVEYKGLNVGMVSDVPYFDRNDSLHLFENGWIPVRIRIEPSRLEINADEQS 310 320 330 340 350 360 370 380 390 400 410 420 ml54.pep KEHWKQQFQTALNKGLTATISSNNLLTGSKMIELNDQPSASPKLRPHTVYAGDTVIATQG gl54 KEHWKQQFQTALNKGLTATISSNNLLTGGKMIELNDQPSASPKLRPHTVYAGDTVIATRG 370 380 390 400 410 420 430 440 450 460 470 480 ml54.pep GGLDDLQVKLADLLDKFDKLPLDKTVAELNGSLAELKSTLKSANAALSSIDKLVGKPQTQ gl54 GGLDDLQVKLADLLDKFNNLPLDKTVAELNGSLAELKΞALKSANAALSSIDKLVGNPQTQ 430 440 450 460 470 480 490 500 510 520 530 540 ml54.pep NIPNELNQTLKELRTTLQGVSPQSPIYGDVQNTLQSLDKTLKDVQPVINTLKEKPNALIF gl54 NIPNELNQTLKELRITLQGVSPQSPIYGDVQNTLQSLDKTLKDVQPVINTLKEKPNALIF 490 500 510 520 530 540 550 ml54.pep NSSSKDPIPKGSRX gl54 NNSSKDPIPKGSRX 550 The following partial DNA sequence was identified in N meningitidis <SEQ ID 635>: al54. seq 1 ATGACTGACA ACAGCCCTCC TCCAAACGGA CACGCCCAAG CACGCGTCCG 51 CAAAAACAAC ACCTTCCTCT CTGCCGTCTG GCTGGTTCCG CTGATCGCGC 101 TGATTGCCGG CGGCTGGCTT TGGGTTAAGG AAATCCGCAA CAGGGGGCCT 151 GTGGTTACGC TCTTGATGGA CAGCGCGGAA GGCATTGAGG TCAACAATAC 201 GGTCATCAAA GTATTGAGCA TCGATGTCGG ACGCGTTACC CGAATCAAAC 251 TGCGCGACGA CCAAAAAGGC GTGGAAGTAA CCGCCCAACT CAATGCGGAC 301 GTATCCGGCC TCATCCGCAG CGATACCCAG TTTTGGGTGG TCAAGCCGCG 351 TATCGACCAA AGCGGCGTAA CCGGTTTGGG TACGCTGCTT TCGGGTTCGT 401 ACATCGCCTT TACACCCGGC AAAAGCGACG AGGCAAAAGA CGTGTTCCAA 451 GTGCAGGACA TTCCGCCCGT TACCGCCATC GGGCAAAGCG GGCTGCGCTT 501 GAATTTGATT GGTAAAAACG ACCGCATCCT CAACGTCAAC AGCCCTGTTT 551 TGTATGAAAA CTTTATGGTC GGGCAAGTCG AAAGCGCGCA TTTCGACCCG 601 TCCGACCAAA GCGTGCATTA CACCATCTTC ATCCAAAGCC CCAACGACAA 651 ACTGATTCAT TCCGCCAGCC GTTTCTGGCT GGAAAGCGGC ATCAATATCG 701 AAACCACAGG CAGCGGCATC AAACTCAATT CCGCCCCTCT GCCTGCCCTG 751 CTGTCGGGCG CGATTTCATT TGATTCGCCG AAAACCAAAA ACAGTAAAAA 801 CGTCAAAAGC GAAGACAGCT TCACGCTTTA CGACAGCCGC AGCGAAGTCG 851 CCAACCTGCC TGATGACCGT TCGCTGTACT ACACCGCGTT TTTCAAACAA 901 TCCGTGCGCG GACTGACCGT CGGTTCGCCT GTCGAGTACA AAGGGCTGAA 951 TGTCGGCGTG GTTTCCGATG TTCCTTATTT CGACCGCAAC GACAGCCTGC 1001 ACCTGTTTGA AAACGGCTGG ATTCCCGTAC GCATCCGTAT TGAGCCTTCC 1051 CGTTTGGAAA TCAATGCCGA CGAACAAAGC AAAGAACATT GGAAACAACA 1101 ATTTCAGACG GCCTTAAACA AAGGCCTGAC CGCCACCATC TCCAGCAACA 1151 ACCTGCTGAC CGGCAGCAAA ATGATTGAGT TGAACGATCA GCCTTCCGCC 1201 TCGCCCAAGC TGCGACCGCA TACCGTTTAT GCAGGCGATA CCGTTATCGC 1251 GACCCAGGGC GGCGGTTTGG ACGATTTGCA GGTCAAATTG GCGGATTTGC 1301 TGGACAAGTT CGACAAACTG CCTTTAGATA AGACGGTTGC CGAATTGAAC 1351 GGTTCGCTTG CCGAGCTCAA ATCCACACTC AAATCTGCCA ATGCCGCCCT 1401 AAGCTCCATC GACAAACTGG TCGGCAAACC GCAGACACAA AACATTCCGA 1451 ACGAACTGAA CCAAACCCTG AAAGAGTTGC GCACAACCCT GCAAGGCGTA 1501 TCGCCTCAAT CGCCTATCTA CGGCGACGTA CAAAATACGC TGCAAAGTTT 1551 GGACAAAACC TTAAAAGACG TTCAACCCGT CATTAACACT TTGAAAGAAA 1601 AACCCAACGC GCTGATTTTC AACAGCAGCA GCAAAGACCC TATCCCGAAA 1651 GGAAGCCGAT AA This corresponds to the amino acid sequence <SEQ ID 636; ORF 154.a>: al54.pep 1 MTDΝSPPPΝG HAQARVRKΝΝ TFLSAVWLVP LIALIAGGWL WVKEIRΝRGP 51 WTLLMDSAE GIEVΝΝTVIK VLSIDVGRVT RIKLRDDQKG VEVTAQLΝAD 101 VSGLIRSDTQ FW KPRIDQ SGVTGLGTLL SGSYIAFTPG KSDEAKDVFQ 151 VQDIPPVTAI GQSGLRLNLI GKNDRILNVN SPVLYENFMV GQVESAHFDP 201 SDQSVHYTIF IQSPNDKLIH SASRFWLESG INIETTGSGI KLNSAPLPAL 251 LSGAISFDSP KTKNSKNVKS EDSFTLYDSR SEVANLPDDR SLYYTAFFKQ 301 SVRGLTVGSP VEYKGLNVGV VSDVPYFDRN DSLHLFENGW IPVRIRIEPS 351 RLEINADEQS KEHWKQQFQT ALNKGLTATI SSNNLLTGSK MIELNDQPSA 401 SPKLRPHTVY AGDTVIATQG GGLDDLQVKL ADLLDKFDKL PLDKTVAELN 451 GSLAELKSTL KSANAALSSI DKLVGKPQTQ NIPNELNQTL KELRTTLQGV 501 SPQSPIYGDV QNTLQSLDKT LKDVQPVINT LKEKPNALIF NSSSKDPIPK 551 GSR* /al 54 100.0% identity in 553 aa overlap 10 20 30 40 50 60 ml54. pep MTDNSPPPNGHAQARVRKNNTFLSAVWLVPLIALIAGGWLWVKEIRNRGPWTLLMDSAE I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al54 MTDNSPPPNGHAQARVRKNNTFLSAVWLVPLIALIAGGWLWVKEIRNRGPWTLLMDSAE 10 20 30 40 50 60 70 80 90 100 110 120 ml54.pep GIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVSGLIRSDTQFWWKPRIDQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al54 GIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVSGLIRSDTQFWWKPRIDQ 70 80 90 100 110 120 130 140 150 160 170 180 ml54.pep SGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAIGQSGLRLNLIGKNDRILNVN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I al54 SGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAIGQSGLRLNLIGKNDRILNVN 130 140 150 160 170 180 190 200 210 220 230 240 ml54.pep SPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKLIHSASRFWLESGINIETTGSGI I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I al54 SPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKLIHSASRFWLESGINIETTGSGI 190 200 210 220 230 240 250 260 270 280 290 300 ml54. pep KLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTLYDSRSEVANLPDDRSLYYTAFFKQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al54 KLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTLYDSRSEVANLPDDRSLYYTAFFKQ 250 260 270 280 290 300 310 320 330 340 350 360 ml54 . pep SVRGLTVGSPVEYKGLNVGWSDVPYFDRNDSLHLFENGWIPVRIRIEPSRLEINADEQS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al54 SVRGLTVGSPVEYKGLNVGWSDVPYFDRNDSLHLFENGWIPVRIRIEPSRLEINADEQS 310 320 330 340 350 360 370 380 390 400 410 420 ml54 . pep KEHWKQQFQTALNKGLTATISSNNLLTGSKMIELNDQPSASPKLRPHTVYAGDTVIATQG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al54 KEHWKQQFQTALNKGLTATISSNNLLTGSKMIELNDQPSASPKLRPHTVYAGDTVIATQG 370 380 390 400 410 420 430 440 450 460 470 480 ml54.pep GGLDDLQVKLADLLDKFDKLPLDKTVAELNGSLAELKSTLKSANAALSSIDKLVGKPQTQ I I I I I I I I I I I I II I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I a154 GGLDDLQVKLADLLDKFDKLPLDKTVAELNGSLAELKSTLKSANAALSSIDKLVGKPQTQ 430 440 450 460 470 480 490 500 510 520 530 540 ml54. pep NIPNELNQTLKELRTTLQGVSPQSPIYGDVQNTLQSLDKTLKDVQPVINTLKEKPNALIF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I al54 NIPNELNQTLKELRTTLQGVSPQSPIYGDVQNTLQSLDKTLKDVQPVINTLKEKPNALIF 490 500 510 520 530 540 550 ml54.pep NSSSKDPIPKGSRX I I I I I I I I I I I I I I al54 NSSSKDPIPKGSRX 550 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 637>: gl55. seq 1 atGAAaatcg GtatcCCACG CGAGTCAtta tcCGGCGAAA cccgcgtagc 51 ctgcAcgccc gCCACCGTTG CCctgctggg caAactAGGC TTTGAAACCG 101 TTGtcgaAAG CGGTGCAggt TTGGCGGCAA GTTTggaCGA TGCCGCTTAC 151 CAAACAGCAG GCGCAACCGT TGCCGACAAA GCGGCGGTTT GGGCCTGCCC 201 TTTAATTTAT AAGGTCAACG CGCCGTCCGA AGGCGAGCTG CCGCTGCTCA 251 AAGAAGGTCA AACCATCGTC AGCTTCCTGT GGCCGCGCCA AAACGAGGCT 301 TTGGTCGAGG CCTTGCGCGC CAAGAAAGTC AACGCGCTGG CGATGGACAT 351 GGTTCCCCGC ATTTCCCGCG CTCAGGCCTT GGACGCTTTG TCTTCAATGG 401 CAAACATCAG CGGCTACCGC GCCGTGATTG AAGCCGCCAA CGCCTTCGGC 451 CGTTTCTTCA CCGGTCAAAT CACTGCCGCC GGCAAAGTGC CGCCTGCGCA 501 GGTTTTGGTG ATTGGCGCCG GTGTGGCGGG TTTGGCGGCA ATCGGTACGG 551 CAAATTCGCT CGGCGCAGTG GTGCGCGCGT TCGATACCCG CTTGGAAGTG 601 GCGGAACAAA TCGAATCGAT GGGCGGTAAG TTcctGAAAC TCGACTTCCT 651 GCAAGAATCG GGCGGCAGCG GAGACGgctA CGCCAAAGTG ATGAGCGACG 701 AATTTATCGC CGCCGAAATG AAGCTCTTTG CCGAACAGGC GAAAGAAGTG 751 GACATCATCA TCACCACCGC CGCCATTCCG GGCAAACCCG CTCCCAAGCT 801 GATTACCAAA GAAATGGTGG AAAGCATGAA ATCCGGATCC GTCATCGTCG 851 ATTTGGCGGC GACGGGCGGC AACTGCGAAC TCACCCGACC GGGCGAATTG 901 TCCGTAACCG GCAACGGCGT GAAAATCATC GGCTACACCG ACATGGCAAA 951 CCGCCTTGCC GGACAGTCTT CCCAGCTTTA CGCCACCAAC TTGGTGAACC 1001 TGACCAAGCT GTTAAGCCCG AACAAAGAcg gcgaAATCAC GCTGGACTTC 1051 GAAGacgtGA TTATCCGCAA TATGACCGTT ACCCGcgacg gcgaaATCAC 1101 CTTCCCGCCT CCGccgaTTc aggtTTCcgc ccggccgCAG CAAAcgccgt 1151 ctgaAAAagc cgcGCCTGCC GCCAagcccg AgccGaaacc tgttCCcctg 1201 tggaAAAaac tcgCGCCCGC CGCcatcgCC GCCGTATTGG tgctgtgGgt 1251 cggCgcggtc gcacccgcag CATTCTTGAA CCACTTTATC GTCTTCGTCC 1301 TCGCCTGCGT CATCGGCTAC CATGTCGTTT GgaacgTCAG CCACTCGCTG 1351 CACACACCGC TGAtgtcggt aaccaaCgcc atctccGGCA tcatggtcgt 1401 cggCGCGCTG CTGCAAATCG GTCAGGGcaa cggcttcgtT TCgctGCTGT 1451 CGTTTGTTGC CATCCTGATT GCCGGCATCA ATATCTTCGG CGGCTTTGCG 1501 GTTACACGGC GTATGCTGAA TATGTTTAAG AAAGGGTAA This corresponds to the amino acid sequence <SEQ ID 638; ORF 155.ng>: gl55.pep 1 MKIGIPRESL SGETRVACTP ATVALLGKLG FETWESGAG LAASLDDAAY 51 QTAGATVADK AAVWACPLIY KVΝAPSEGEL PLLKEGQTIV SFLWPRQΝEA 101 LVEALRAKKV ΝALAMDMVPR ISRAQALDAL SSMAΝISGYR AVIEAAΝAFG 151 RFFTGQITAA GKVPPAQVLV IGAGVAGLAA IGTAΝSLGAV VRAFDTRLEV 201 AEQIESMGGK FLKLDFLQES GGSGDGYAKV MSDEFIAAEM KLFAEQAKEV 251 DIIITTAAIP GKPAPKLITK EMVESMKSGS VIVDLAATGG ΝCELTRPGEL 301 SVTGΝGVKII GYTDMAΝRLA GQSSQLYATΝ LVΝLTKLLSP ΝKDGEITLDF 351 EDVIIRΝMTV TRDGEITFPP PPIQVSARPQ QTPSEKAAPA AKPEPKPVPL 401 WKKLAPAAIA AVLVLWVGAV APAAFLΝHFI VFVLACVIGY HWWΝVSHSL 451 HTPLMSVTΝA ISGIM GAL LQIGQGΝGFV SLLSFVAILI AGIΝIFGGFA 501 VTRRMLΝMFK KG* The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 639>: ml55. seq 1 ATGAAAATCG GTATCCCACG CGAGTCATTA TCCGGCGAAA CCCGCGTCGC 51 CTGTACGCCC GCCACCGTCG CCCTGCTGGG CAAACTGGGC TTTGAAACCG 101 TTGTCGAAAG CGGTGCAGGT TTGGCGGCAA GTTTGGACGA TGCCGCTTAC 151 CAAACAGCAG GCGCAACCGT TGCCGACAAA GCGGCGGTTT GGGTCTGCCC 201 TTTGATTTAT AAGGTCAACG CGCCGTCCGA ACAGGAACTG CCGCTTTTGA 251 ACGAAGGTCA AACCATCGTC AGCTTCCTGT GGCCGCGCCA AAACGAGGCT 301 TTGGTCGAAG CCTTGCGCGC CAAGAAAGTG AACGCGCTGG CGATGGATAT 351 GGTGCCCCGC ATTTCGCGCG CGCAGGCTTT GGACGCTTTG TCTTCGATGG 401 CAAACATCAG CGGCTACCGC GCCGTAATTG AAGCCGCCAA CGCCTTCGGC 451 CGTTTCTTCA CCGGTCAAAT TACCGCCGCC GGCAAAGTGC CGCCCGCGCA 501 GGTTTTGGTG ATTGGTGCAG GTGTGGCAGG TTTGGCGGCG ATCGGTACGG 551 CAAACTCGCT CGGCGCAGTG GTACGCGCGT TCGATACCCG CTTGGAAGTG 601 GCGGAACAAA TCGAATCGAT GGGCGGCAAG TTCCTGAAAC TCGACTTCCC 651 ACAAGAATCG GGCGGCAGCG GAGACGGCTA CGCCAAAGTG ATGAGCGACG 701 AATTTATCGC AGCCGAGATG AAGCTCTTTG CCGAGCAGGC GAAAGAAGTG 751 GACATCATCA TCACCACCGC CGCCATTCCG GGCAAACCCG CGCCCAAGCT 801 GATTACCAAA GAAATGGTGG AAAGCATGAA ATCCGGCTCC GTCATCGTCG 851 ATTTGGCGGC GGCGACGGGC GGCAACTGCG AACTCACCCG CCCGGGCGAA 901 TTGTCCGTAA CCGGCAACGG CGTGAAAATC ATCGGCTACA CCGACATGGC 951 AAACCGCCTT GCCGGACAGT CTTCCCAGCT TTACGCCACC AACTTGGTCA 1001 ACCTGACCAA GCTGTTAAGC CCGAACAAAG ACGGCGAAAT CACGTTGGAC 1051 TTCGAAGACG TGATTATCCG CAACATGACC GTTACCCACG ACGGCGAAAT 1101 CACCTTCCCG CCTCCGCCGA TTCAAGTTTC CGCCCAGCCG CAGCAAACGC 1151 CGTCTGAAAA AGCCGTGCCT GCCGCCAAGC CCGAGCCAAA ACCCGTTCCC 1201 CTGTGGAAAA AACTCGCGCC CGCCGTCATC GCCGCCGTCT TGGTACTGTG 1251 GGTCGGCGCG GTCGCACCCG CAGCATTCCT GAACCACTTT ATCGTGTTCG 1301 TTCTCGCCTG CGTCATCGGC TACTACGTCG TCTGGAACGT CAGCCACTCG 1351 CTGCACACAC CGCTGATGTC GGTAACCAAC GCCATCTCCG GCATCATCGT 1401 CGTCGGCGCG CTGCTGCAAA TCGGTCAGGG CAACGGCTTC GTTTCGCTGC 1451 TGTCGTTTGT TGCCATCCTG ATTGCCGGCA TCAACATCTT CGGCGGCTTT 1501 GCGGTAACAC GGCGTATGCT GAATATGTTT AAGAAAGGGT AA This corresponds to the amino acid sequence <SEQ ID 640; ORF 155>: ml55.pep 1 MKIGIPRESL SGETRVACTP ATVALLGKLG FETWESGAG LAASLDDAAY 51 QTAGATVADK AAVWVCPLIY KVNAPSEQEL PLLNEGQTIV SFLWPRQNEA 101 LVEALRAKKV NALAMDMVPR ISRAQALDAL SSMANISGYR AVIEAANAFG 151 RFFTGQITAA GKVPPAQVLV IGAGVAGLAA IGTANSLGAV VRAFDTRLEV 201 AEQIESMGGK FLKLDFPQES GGSGDGYAKV MSDEFIAAEM KLFAEQAKEV 251 DIIITTAAIP GKPAPKLITK EMVESMKSGS VIVDLAAATG GNCELTRPGE 301 LSVTGNGVKI IGYTDMANRL AGQSSQLYAT NLVNLTKLLS PNKDGEITLD 351 FEDVIIRNMT VTHDGEITFP PPPIQVSAQP QQTPSEKAVP AAKPEPKPVP 401 LWKKLAPAVI AAVLVLWVGA VAPAAFLNHF IVFVLACVIG YYWWNVSHS 451 LHTPLMSVTN AISGIIVVGA LLQIGQGNGF VSLLSFVAIL IAGINIFGGF 501 AVTRRMLNMF KKG* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 155 shows 97.9% identity over a 513 aa overlap with a predicted ORF (ORF 155.ng) from N gonorrhoeae: ml55 / gl55 97.9% identity in 513 aa overlap 10 20 30 40 50 60 ml55. pep MKIGIPRESLSGETRVACTPATVALLGKLGFETWESGAGLAASLDDAAYQTAGATVADK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I gl55 MKIGIPRESLSGETRVACTPATVALLGKLGFETWESGAGLAASLDDAAYQTAGATVADK 10 20 30 40 50 60 70 80 90 100 110 120 ml55. pep AAVWVCPLIYKVΝAPSEQELPLLΝEGQTIVSFLWPRQΝEALVEALRAKKVΝALAMDMVPR I I I I: I I I I I I I I I I I I I I I I I: I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl55 AAVWACPLIYKVΝAPSEGELPLLKEGQTIVSFLWPRQΝEALVEALRAKKVΝALAMDMVPR 70 80 90 100 110 120 130 140 150 160 170 180 ml55. pep ISRAQALDALSSMAΝISGYRAVIEAAΝAFGRFFTGQITAAGKVPPAQVLVIGAGVAGLAA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl55 ISRAQALDALSSMAΝISGYRAVIEAAΝAFGRFFTGQITAAGKVPPAQVLVIGAGVAGLAA 130 140 150 160 170 180 190 200 210 220 230 240 ml55. ■pep IGTANSLGAWRAFDTRLEVAEQIESMGGKFLKLDFPQESGGSGDGYAKVMSDEFIAAEM I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl55 IGTANSLGAWRAFDTRLEVAEQIESMGGKFLKLDFLQESGGSGDGYAKVMSDEFIAAEM 190 200 210 220 230 240 250 260 270 280 290 300 ml55. .pep KLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESMKSGSVIVDLAAATGGNCELTRPGE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl55 KLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESMKSGSVIVDLAA-TGGNCELTRPGE 250 260 270 280 290 310 320 330 340 350 360 ml55. •pep LSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVNLTKLLΞPNKDGEITLDFEDVIIRNMT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl55 LSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVNLTKLLSPNKDGEITLDFEDVIIRNMT 300 310 320 330 340 350 370 380 390 400 410 420 ml55. pep VTHDGEITFPPPPIQVSAQPQQTPSEKAVPAAKPEPKPVPLWKKLAPAVIAAVLVLWVGA I I : I I I I I I I I I I I I I I I : I I I I I I I I I : I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I gl55 VTRDGEITFPPPPIQVSARPQQTPSEKAAPAAKPEPKPVPLWKKLAPAAIAAVLVLWVGA 360 370 380 390 400 410 430 440 450 460 470 480 ml55 . pep VAPAAFLNHFIVFVLACVIGYYWWNVSHSLHTPLMSVTNAISGIIWGALLQIGQGNGF I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I II I I I I I I I I: I I I I I I I I I I I I I I gl55 VAPAAFLNHFIVFVLACVIGYHWWNVSHSLHTPLMSVTNAISGIMWGALLQIGQGNGF 420 430 440 450 460 470 490 500 510 ml55.pep VSLLSFVAILIAGINIFGGFAVTRRMLNMFKKGX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl55 VSLLSFVAILIAGINIFGGFAVTRRMLNMFKKGX 480 490 500 510 The following partial DNA sequence was identified in N meningitidis <SEQ ID 641>: al55. seq 1 ATGAAAATCG GTATCCCACG TGAGTCATTA TCCGGCGAAA CCCGCGTCGC 51 CTGTACGCCC GCCACCGTCG CCCTGCTGGG CAAACTGGGC TTTGAAACCG 101 TTGTCGAAAG CGGCGCAGGT TTGGCGGCAA GTTTGGACGA TGCCGCTTAC 151 CAAGCAGCAG GCGCAACCGT TGCCGACAAA GCAGCGGTTT GGGCATACCC 201 TTTAATTTAT AAGGTTAACG CGCCGTCCGA AGACGAGCTG CCGCTGCTCA 251 AAGAAGGACA GACCATCGTC AGCTTCCTGT GGCCGCGCCA AAACGAGGCT 301 TTGGTCGAAG CCTTGCGCGC CAAGAAAGTG AACGCGCTGG CAATGGACAT 351 GGTGCCCCGC ATTTCGCGCG CGCAGGCTTT GGACGΝTTTG TCTTΝGATGG 401 CAAACATCAG CGGCTACCGC GCCGTGATTG AAGCCGCCAA CGCCTTCGGC 451 CGTTTNTTCA CCGGCCAAAT TACTGCCGCA GGCAAAGTGC CGCCCGCGCA 501 GGTTTTGGTG ATTGGTGCAG GTGTGGCAGG TTTGGCGGCG ATCGGTACGG 551 CAAACTCGCT CGGCGCAGTG GTACGCGTGT TCGATACCCG CCTG . AAGTG 601 GCGGAACAAT TAGAATCGAT GGGCGGCAAG TTCCTGAAAC TCGACTTCCC 651 GCAAGAATCG GGCGGCAGCG GCGACGGCTA CGCCAAAGTG ATGAGCGACG 701 AATTTATCGC CGCCGAGATG AAGCTTTTTG CCGAGCAGGC GAAAGAAGTG 751 GACATCATCA TCACCACCGC CGCCATTCCG GGCAAACCCG CGCCCAAGCΝ 801 NNTNANCAAA GAAATGGTCG AAAGCATGAA ACCCGGCTCC GTCATCGTCG 851 ATTTGGCGGC GGCGACGGGC GGCAACTGCG AACTCACCAA ACAGGGCGAA 901 TTGTTCGTAA CCGGCAACGG CGTGAAAATC ATCGGCTACA CCGACATGGC 951 AAACCGCCTT GCCGGACAGT CTTCGCAGCT TTACGCCACC AACTTGGTCA 1001 ACCTGACCAA GCTGTTAAGC CCGAACAAAG ACGGCGAAAT CACGCTGGAC 1051 TTCGAAGACG TGATTATCCG CAACATGACC GTTACCCGCG ACGGCGAAAT 1101 CACCTTCCCG CCTCCGCCGA TTCAAGTTTC CGCCCAACCG CAGCAAACGC 1151 CGTCTGAAAA AGCCGCGCCT GCCGCCAAGC CCGAACCGAA ACCCGTTCCC 1201 CTGTGGAAAA AACTCGCGCC CGCCNTNATC GCCGCCGTGT TGGTACTGTG 1251 GGTCGGCGCG GTCGCACCCG CAGCATTCCT GAACCACTTT ATCGTCTTCG 1301 TCCTCGCCTG CGTCATCGGC TACTATGTCG TTTGGAACGT CAGCCACTCG 1351 CTGCACACAC CGCTGATGTC GGTGACCAAC GCCATTTCCG GCATCATCGT 1401 CGTCGGCGCG CTGCTGCAAA TCGGTCAGGG CAACGGCTTC GTTTCGCTGC 1451 TGTCGTTTGT TGCCATCCTG ATTGCCAGCA TCAACATCTT CGGCGGCTTC 1501 TTTGTAACGC GGCGGATGCT GAATATGTTT AGGAAAGGGT AA This corresponds to the amino acid sequence <SEQ ID 642; ORF 155.a>: al55.pep 1 MKIGIPRESL SGETRVACTP ATVALLGKLG FETWESGAG LAASLDDAAY 51 QAAGATVADK AAVWAYPLIY KVNAPSEDEL PLLKEGQTIV SFLWPRQNEA 101 LVEALRAKKV NALAMDMVPR ISRAQALDXL SXMANISGYR AVIEAANAFG 151 RXFTGQITAA GKVPPAQVLV IGAGVAGLAA IGTANSLGAV VRVFDTRLXV 201 AEQLESMGGK FLKLDFPQES GGSGDGYAKV MSDEFIAAEM KLFAEQAKEV 251 DIIITTAAIP GKPAPKXXXK EMVESMKPGS VIVDLAAATG GNCELTKQGE 301 LFVTGNGVKI IGYTDMANRL AGQSSQLYAT NLVNLTKLLS PNKDGEITLD 351 FEDVIIRNMT VTRDGEITFP PPPIQVSAQP QQTPSEKAAP AAKPEPKPVP 401 LWKKLAPAXI AAVLVLWVGA VAPAAFLNHF IVFVLACVIG YYWWNVSHS 451 L LHHTTPPLLMMSSVVTTNN AAIISSGGIIIIVVVVGGAA LLLLQQIIGGQQGGNNGGFF VVSSLLLLSSFFVVAAIILL IASINIFGGF 501 FVTRRMLNMF RKG* ml55/al55 95 . 3% identity in 513 aa overlap 10 20 30 40 50 60 ml55. pep MKIGIPRESLSGETRVACTPATVALLGKLGFETWESGAGLAASLDDAAYQTAGATVADK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I al55 MKIGIPRESLSGETRVACTPATVALLGKLGFETWESGAGLAASLDDAAYQAAGATVADK 10 20 30 40 50 60 70 80 90 100 110 120 ml55. pep AAVWVCPLIYKVNAPSEQELPLLNEGQTIVSFLWPRQNEALVEALRAKKVNALAMDMVPR MM: II II I I I I I II : I M I I : I M M I I I II II I I I II I I II II I I II II I II I II I al55 AAVWAYPLIYKVNAPSEDELPLLKEGQTIVSFLWPRQNEALVEALRAKKVNALAMDMVPR 70 80 90 100 110 120 130 140 150 160 170 180 ml55. pep ISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAAGKVPPAQVLVIGAGVAGLAA llllllll II II I I I I II I I I I I II I I I I II I II I II II I I I II I I II II I II I II I al55 ISRAQALDXLSXMANISGYRAVIEAANAFGRXFTGQITAAGKVPPAQVLVIGAGVAGLAA 130 140 150 160 170 180 190 200 210 220 230 240 ml55. pep IGTANSLGAWRAFDTRLEVAEQIESMGGKFLKLDFPQESGGSGDGYAKVMSDEFIAAEM I I I II II II II I : I I I I I I II I : I II I I I II I II II I II II I I I I I I II I II I II I II I al55 IGTANSLGAWRVFDTRLXVAEQLESMGGKFLKLDFPQESGGSGDGYAKVMSDEFIAAEM 190 200 210 220 230 240 250 260 270 280 290 300 ml55. pep KLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESMKSGSVIVDLAAATGGNCELTRPGE I I I II II II II I I I I I I I II II II I I : I II II I I I II II I I I II I I I I II I II : II al55 KLFAEQAKEVDIIITTAAIPGKPAPKXXXKEMVESMKPGSVIVDLAAATGGNCELTKQGE 250 260 270 280 290 300 310 320 330 340 350 360 ml55. pep LSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVNLTKLLSPNKDGEITLDFEDVIIRNMT I I II II II II I I I I I I I II II II I II II II I II I I I I I II II II II I I II I II I II I II al55 LFVTGNGVKIIGYTDMANRLAGQSSQLYATNLVNLTKLLSPNKDGEITLDFEDVIIRNMT 310 320 330 340 350 360 370 380 390 400 410 420 ml55. pep VTHDGEITFPPPPIQVSAQPQQTPSEKAVPAAKPEPKPVPLWKKLAPAVIAAVLVLWVGA II : I II II II I I I I I I I I I I I I I I I I II : I I I I I I II I M I I I I I I I I I I II I II I II I al55 VTRDGEITFPPPPIQVSAQPQQTPSEKAAPAAKPEPKPVPLWKKLAPAXIAAVLVLWVGA 370 380 390 400 410 420 430 440 450 460 470 480 ml55. pep VAPAAFLNHFIVFVLACVIGYYWWNVSHSLHTPLMSVTNAISGIIWGALLQIGQGNGF I I II I I I I II II I I I I I I I I I II I I I II I I I I II II II II I II II I I I I II II II II I I I al55 VAPAAFLNHFIVFVLACVIGYYWWNVSHSLHTPLMSVTNAISGIIWGALLQIGQGNGF 430 440 450 460 470 480 490 500 510 ml55. pep VSLLSFVAILIAGINIFGGFAVTRRMLNMFKKGX I II II I I II II I : I I I I II I I I I I I I II I : I I I al55 VSLLSFVAILIASINIFGGFFVTRRMLNMFRKGX 490 500 510 The following partial DNA sequence was identified in N gonorrhoeae <SEQ LD 643>: gl56. seq 1 ATGACTTTCG CCTATTGGTG CATTCTGATT GCCTGCCTAT TGCCGCTTTT 51 TTGTGCGGCG TATGCCAAAA AAGCGGGCGG ATTCCGGTTT AAAGACAACC 101 ACAATCCTCG CGGTTTTCTG GCACATACGC AAGGCGCAGC CGCCCGTGCC 151 CACGCCGCGC AGCAAAACGG TTTTGAAGCC TTTGCACCGT TTGCCGCCGC 201 CGTTTTGACG GCACACGCAA CCGGCAATGC CGGACAAGCA ACCGTCAACA 251 CGCTTGCCGG ATTGTTCATC CTGTTCCGCC TCGCCTTTAT CTGGTGCTAC 301 ATCGCAGACA AAGCAGCATT GCGCTCGCTG ATGTGGGCGG GCGGATTTGC 351 CTGCACCGTC GGACTGTTTG TCGCGGCTGC TTGA This corresponds to the amino acid sequence <SEQ ID 644; ORF 156.ng>: gl56.pep 1 MTFAYWCILI ACLLPLFCAA YAKKAGGFRF KDΝHΝPRGFL AHTQGAAARA 51 HAAQQΝGFEA FAPFAAAVLT AHATGΝAGQA TVΝTLAGLFI LFRLAFIWCY 101 IADKAALRSL MWAGGFACTV GLFVAAA* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 645>: ml56. seq 1 ATGACTTTCG CCTATTGGTG TATTCTGATT GCCTGCCTAT TGCCGCTTTT 51 TTGTGCGGCG TATGCCAAAA AAGCGGGCGG ATTCCGGTTT AAAGACAACC 101 ACAATCCGCG CGGTTTTCTA GCGCACACGC AAGGCGCAGC CGCCCGTGCC 151 CACGCCGCAC AGCAAAACGG TTTTGAAGCC TTTGCACCGT TTGCCGCCGC 201 CGTTTTGACG GCACACGCAA CCGGCAATGC GGCGCAATCG ACCATCAACA 251 CGCTTGCCTG CCTGTTCATC CTGTTCCGCC TCGCCTTTAT CTGGTGCTAT 301 ATCGCCGACA AAGCCGCTAT GCGCTCACTG ATGTGGGCAG GCGGATTTGC 351 CTGCACCGTC GGGCTGTTTG TCGCGGCTGC TTGA This corresponds to the amino acid sequence <SEQ ID 646; ORF 156>: ml56.pep 1 MTFAYWCILI ACLLPLFCAA YAKKAGGFRF KDΝHΝPRGFL AHTQGAAARA 51 HAAQQΝGFEA FAPFAAAVLT AHATGΝAAQS TIΝTLACLFI LFRLAFIWCY 101 IADKAAMRSL MWAGGFACTV GLFVAAA* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ml56 / gl56 96.1% identity in 127 aa overlap 10 20 30 40 50 60 ml56.pep MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDΝHΝPRGFLAHTQGAAARAHAAQQΝGFEA I II II I I II II II II I I I I I I I I II I I I II I I II II I I II II I I I II II I I I II II I I I I gl56 MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDΝHΝPRGFLAHTQGAAARAHAAQQΝGFEA 10 20 30 40 50 60 70 80 90 100 110 120 ml56. pep FAPFAAAVLTAHATGΝAAQSTIΝTLACLFILFRLAFIWCYIADKAAMRSLMWAGGFACTV II II I II I II I I I I I II: I : I : I I I I II I II I II II I I I II I I I I : I II I I I I I II I II gl56 FAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIADKAALRSLMWAGGFACTV 70 80 90 100 110 120 ml56. pep GLFVAAAX I II I M II gl56 GLFVAAAX The following partial DNA sequence was identified in N meningitidis <SEQ ID 647>: al56. seq 1 ATGACTTTCG CCTATTGGTG TATTCTGATT GCCTACCTAT TGCCGCTTTT 51 TTGTGCGGCG TATGCCAAAA AAGCGGGCGG ATTCCGGTTT AAAGACAACC 101 ACAATCCGCG CGATTTTCTG GCGCGCACGC AAGGCACAGC CGCCCGTGCC 151 CACGCCGCGC AGCAAAACGG TTTTGAAGCC TTTGCACCGT TTGCAGCCGC 201 CGTTTTGACG GCACACGCAA CCGGCAATGC CGGACAAGCA ACCGTCAACA 251 CGCTTGCCGG CCTGTTCATC CTGTTCCGCC TCGCCTTTAT CTGGTGCTAC 301 ATCGCAGACA AAGCAGCATT ACGCTCGCTG ATGTGGGTGG GCGGATTTGT 351 CTGCACCGTC GGGCTGTTTG TCGTGGCTGC TTGA This corresponds to the amino acid sequence <SEQ ID 648; ORF 156.a>: al56.pep 1 MTFAYWCILI AYLLPLFCAA YAKKAGGFRF KDΝHΝPRDFL ARTQGTAARA 51 HAAQQΝGFEA FAPFAAAVLT AHATGΝAGQA TVΝTLAGLFI LFRLAFIWCY 101 IADKAALRSL MWVGGFVCTV GLFWAA* ml56/al56 90.6% identity in 127 aa overlap 10 20 30 40 50 60 ml56. pep MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDΝHΝPRGFLAHTQGAAARAHAAQQΝGFEA II I I II II I I I I I II I I II II II I I I I II I I II I I I I II : I I I : II I II I II II II I I al56 MTFAYWCILIAYLLPLFCAAYAKKAGGFRFKDΝHΝPRDFLARTQGTAARAHAAQQΝGFEA 10 20 30 40 50 60 70 80 90 100 110 120 ml56. pep FAPFAAAVLTAHATGΝAAQSTIΝTLACLFILFRLAFIWCYIADKAAMRSLMWAGGFACTV II II I I I I I I I I I II I I : I : I : I I I I I II II II I I I II I II I I II : I II I I : I I I : II I al56 FAPFAAAVLTAHATGΝAGQATVΝTLAGLFILFRLAFIWCYIADKAALRSLMWVGGFVCTV 70 80 90 100 110 120 ml56. pep GLFVAAAX 1111:111 al56 GLFWAAX The following partial DΝA sequence was identified in N gonorrhoeae <SEQ LD 649>: gl57. seq 1 atgaggaacg aggAAAAACg cgccctgcgc cgcgaattgC gCgGgcggcg 51 ttcgcAAATg GGgcgagacg tGCGggCGGC GGCGgCgatA Aaaatcaacc 101 gcctgctcaa aCGTtatatc AAGCGCggtc gGaAaatcgG CGTGTATTgg 151 cCGATGGGCA AGGAATTGcg TTTGGGCGgc tTtgtcCGCG CGGCGCAAAA 201 ACGCgGCGCA AAactctatc tgccttATAT CGAACCGCAC ACGCGGCGGA 251 TGTGGTTTAC GCCGTATCCT GAACGCGGAA TGGAACGGGA ACGCAAGCGC 301 GGTAGGGCGA AGCTGCATGT CCCTCAGTTT GCAGGGCGCA AAATCCGCGT 351 GCACGGTTTG TCGGTATTGC TCGTCCCGCT TGTCGGCATA GACCGCGAAG 401 GCTACCGTTT GGGGCAGGCA GGCGGCTATT ACGATGCGAC GCTTTCGGCG 451 ATGAAATACC GTTTGCAGGC GAAAACCGTG GGCGTGGGCT TTGCCTGCCA 501 GTTGGTGGAC AGGCTCCCAC GCGAGGCGCA CGACCTGCCG CTGGACGGTT 551 TTGTATCGGA AGCGGGGATA TTGTGTTTTT AG This corresponds to the amino acid sequence <SEQ ID 650; ORF 157.ng>: gl57.pep 1 MRNEEKRALR RELRGRRSQM GRDVRAAAAI KINRLLKRYI KRGRKIGVYW 51 PMGKELRLGG FVRAAQKRGA KLYLPYIEPH TRRMWFTPYP ERGMERERKR 101 GRAKLHVPQF AGRKIRVHGL SVLLVPLVGI DREGYRLGQA GGYYDATLSA 151 MKYRLQAKTV GVGFACQLVD RLPREAHDLP LDGFVSEAGI LCF* The following partial DNA sequence was identified in N meningitidis <SEQ LD 651>: ml57. seq 1 ATGAGGAACG AGGAAAAACG CGCCCTGCGC CGCGAATTGC GCGGGCGGCG 51 TTCGCAAATG GGGCGGGACG TGCGGGCGGC GGCAACGGTA AAAATCAACC 101 ACCTGCTCAA ACGTTATATT AAAAAAGGGC GGAAAATCGG CGTGTATTGG 151 CCGATGGGCA AGGAATTGCG TTTGGACGGC TTTGTCCGCG CGGCGCAAAA 201 ACGCGGTGCG GAACTCTACC TGCCTTATAT CGAACCGCGT TCGCGGCGGA 251 TGTGGTTTAC GCCGTATCCT GCCGATGGAG TAAAACAAGA ACGCAAGCGC 301 GGTAGGGCGA AGCTGCATGT CCCTCAGTTT GCAGGTCGGA AAAAGCGTGT 351 GCATGATTTG AACCTCCTGC TTGTGCCAGT GGTCGGTATG GACAGGCTGG 401 GCTACCGCTT GGGACAGGCA GGCGGCTATT ACGATGCGAC GCTTTCAGCG 451 ATGAAATACC GTTTGCAGGC AAAAACCGTG GGCGTGGGCT TTGCCTGCCA 501 GTTGGTGGAC AGGCTGCCGG TCGAGGCGCA CGACCGGTCT TTGGACGGTT 551 TTGTGTCGGA GGCGGGGATA TTGTGTTTTT AG This corresponds to the amino acid sequence <SEQ ID 652; ORF 157>: ml57.pep 1 MRNEEKRALR RELRGRRSQM GRDVRAAATV KINHLLKRYI KKGRKIGVYW 51 PMGKELRLDG FVRAAQKRGA ELYLPYIEPR SRRMWFTPYP ADGVKQERKR 101 GRAKLHVPQF AGRKKRVHDL NLLLVPWGM DRLGYRLGQA GGYYDATLSA 151 MKYRLQAKTV GVGFACQLVD RLPVEAHDRS LDGFVSEAGI LCF* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae l57 / gi57 88.1% identity in 193 aa overlap 10 20 30 40 50 60 ml57. •pep MRΝEEKRALRRELRGRRSQMGRDVRAAATVKIΝHLLKRYIKKGRKIGVYWPMGKELRLDG II I I II I II I I II I I II II II II II I I I : : II I : II I I I I I : II II II II I II I I II I I gl57 MRΝEEKRALRRELRGRRSQMGRDVRAAAAIKIΝRLLKRYIKRGRKIGVYWPMGKELRLGG 10 20 30 40 50 60 70 80 90 100 110 120 ml57. • pep FVRAAQKRGAELYLPYIEPRSRRMWFTPYPADGVKQERKRGRAKLHVPQFAGRKKRVHDL I I I I I II II I : I II I I I II :: I I I I II I II I ::: I I II I I I I I I I I II I II I II I I gl57 FVRAAQKRGAKLYLPYIEPHTRRMWFTPYPERGMERERKRGRAKLHVPQFAGRKIRVHGL 70 80 90 100 110 120 130 140 150 160 170 180 ml57. •pep ΝLLLVP GMDRLGYRLGQAGGYYDATLSAMKYRLQAKTVGVGFACQLVDRLPVEAHDRS :: I I I I : I I: II I I I I II II I I I I I II II II I II I I I II I I I I II I I II I II I I I I gl57 SVLLVPLVGIDREGYRLGQAGGYYDATLSAMKYRLQAKTVGVGFACQLVDRLPREAHDLP 130 140 150 160 170 180 190 ml57.pep LDGFVSEAGILCFX I I I I I I I I I I II II gl57 LDGFVSEAGILCFX 190 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 653>: al57. seq 1 ATGAGGAACG AGGAAAAACA CGCCTTGCGC CGAGAGTTGC GCCGCGCCCG 51 CGCGCAGATG GGGCATCAAG GGCGGTTGGC GGCGGGGCAA ACGATTAACC 101 GCCTGCTCAA ACGTTATATC AAGCGTGGTC GGAAAATCGG CGTGTATTGG 151 CCGATGGGCA AGGAATTGCG TTTGGACGGC TTTGTCCGCG CGGCGCAAAA 201 ACGCGGTGCA AAACTTTATC TGCCTTATAT CGAACCGCGT TCGCGGCGGA 251 TGTGGTTTAC GCCGTATCCT GAAAGCGGAA TGGAACGGGA GCGCATACGG 301 GGCAGGGCGA AGTTGAACGT GCCGCAGTTT GCAGGGCGCA AAATCCGCGT 351 GCACGGTTTG TCGGTATTGC TCGTCCCGCT TGTCGGCATA GACCGCGAGG 401 GCTACCGCTT AGGACAGGCA GGCGGCTATT ACGATGCGAC GCTTGCGGCG 451 ATGAAATACC GTTTGCAGGC AAAAACCGTG GGCGTGGGCT TTGCCTGCCA 501 GTTTGTGGAC AGGCTGCCGC GCGAACCGCA CGATCTGCTG CTGGACGGTT 551 TTGTGTCGGA GGCGGGGATA TTGTGCTTTT AG This corresponds to the amino acid sequence <SEQ ID 654; ORF 157.a>: al57.pep 1 MRNEEKHALR RELRRARAQM GHQGRLAAGQ TINRLLKRYI KRGRKIGVYW 51 PMGKELRLDG FVRAAQKRGA KLYLPYIEPR SRRMWFTPYP ESGMERERIR 101 GRAKLNVPQF AGRKIRVHGL SVLLVPLVGI DREGYRLGQA GGYYDATLAA 151 MKYRLQAKTV GVGFACQFVD RLPREPHDLL LDGFVSEAGI LCF* ml57/al57 82.4% identity in 193 aa overlap 10 20 30 40 50 60 ml 57 . pep MRNEEKRALRRELRGRRSQMGRDVRAAATVKINHLLKRYIKKGRKIGVYWPMGKELRLDG I I I I I I : I I I I I I I i l l : : I I I M M M M M M M M M M M I M M M al57 MRNEEKHALRRELRRARAQMGHQGRLAAGQTINRLLKRYIKRGRKIGVYWPMGKELRLDG 10 20 30 40 50 60 70 80 90 100 110 120 ml57. pep FVRAAQKRGAELYLPYIEPRSRRMWFTPYPADGVKQERKRGRAKLHVPQFAGRKKRVHDL I II II I II II : I I II I II I II II II II I I I : I :: : I I I I I I II : I II I I I I I III I al57 FVRAAQKRGAKLYLPYIEPRSRRMWFTPYPESGMERERIRGRAKLNVPQFAGRKIRVHGL 70 80 90 100 110 120 130 140 150 160 170 180 ml57. pep NLLLVPWGMDRLGYRLGQAGGYYDATLSAMKYRLQAKTVGVGFACQLVDRLPVEAHDRS :: I I I I : II : II II I II I II II II I I I : I I II I I II I II I I II II I : I II II I M al57 SVLLVPLVGIDREGYRLGQAGGYYDATLAAMKYRLQAKTVGVGFACQFVDRLPREPHDLL 130 140 150 160 170 180 190 ml57.pep LDGFVSEAGILCFX I II II II I II II I I al57 LDGFVSEAGILCFX 190 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 655>: gl58. seq 1 ATGAAAACCA ATTCAGAAGA ACTGACCGTA TTTGTTCAAG TGGTGGAAAG 51 CGGCAGCTTC AGCCGTGCGG CGgagcAGTT GGAGAtggCA AATTCTGCCG 101 TAAGCCGCAT CGTCAAACGG CTGGAGGAAA AGTTGGGCGT GAAcCTGCtc 151 aACCGCACCA CGCGGCAACT CAATCTGACG GAAGAAGGCG CGCAATATTT 201 CCGCCGCGCG CAGAGAATCC TGCAAGAAAT GGCAGCGGCG GAAACCGAAA 251 TGCTGGCAGT GCACGAAGTA CCGCAAGGCG TGTTGCGCGT GGATTCCGCG 301 ATGCcgatgg TGCTGCATCT GCTGGCGCCG CTGGCAGCAA AATTCAACGA 351 ACGCTATCCG CATATCcgaC TTTCGCTCGT TTCTTCCGAa ggctatatca 401 atctGattGA Acgcaaagtc gAtatTGCCT TACGGGCCGG AGAATTGGAC 451 GATTCCGGGC TGCGTGCACG CCATCTGTTT GACAGCCACT TCCGCGtagt 501 cgCCAGTCCT GAATATTTAG CAAAACACGG CACGCCACAA TCTGCAGAAG 551 atcTTGCCAA CCATCAATGT TTAGGCTTCA CAGAACCCGG TTCTCTAAAT 601 ACATGGGCGG TTTTAGAtgC GCAGGGAAAT CCCTATAAAA TTTCACCGCA 651 CTTTACCGCC AGCAGCGGTG AAATCTTACG CTCGTTGTGC CTTTCAAGtt 701 gCGGTATTGC TTGCTTATCA GATTTTTTGG TTGACAACGA CATCACTGAA 751 GGAAAGTTAA TTCCcctatt cgCCGAACAA ACCTCCAATA AAACACACCC 801 CTTTAATGCT GTTTATTACA GCGATAAAGC CGTCAACCTC CGCTTACGCG 851 TATTTTTGGA TTTTTTAGTG AAGGAACTGG GAAAAAATAT GAATAGAACG 901 AATACCAAAT AA This corresponds to the amino acid sequence <SEQ ID 656; ORF 158.ng>: gl58.pep 1 MKTNSEELTV FVQWESGSF SRAAEQLEMA NSAVSRIVKR LEEKLGVNLL 51 NRTTRQLNLT EEGAQYFRRA QRILQEMAAA ETEMLAVHEV PQGVLRVDSA 101 MPMVLHLLAP LAAKFNERYP HIRLSLVSSE GYINLIERKV DIALRAGELD 151 DSGLRARHLF DSHFRWASP EYLAKHGTPQ SAEDLANHQC LGFTEPGSLN 201 TWAVLDAQGN PYKISPHFTA SSGEILRSLC LSSCGIACLS DFLVDNDITE 251 GKLIPLFAEQ TSNKTHPFNA VYYSDKAVNL RLRVFLDFLV KELGKNMNRT 301 NTK* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 657>: ml58. seq 1 ATGAAAACCA ATTCAGAAGA ACTGACCGTA TTTGTTCAAG TGGTGGAAAG 51 CGGCAGCTTC AGCCGTGCGG CGGAGCAGTT GGCGATGGCA AATTCTGCCG 101 TAAGCCGCAT CGTCAAACGG CTGGAGGAAA AGTTGGGTGT GAACCTGCTC 151 AACCGCACCA CGCGGCAACT CAGTCTGACG GAAGAAGGCG CGCAATATTT 201 CCGCCGCGCG CAGAGAATCC TGCAAGAAAT GGCAGCGGCG GAAACCGAAA 251 TGCTGGCAGT GCACGAAATA CCGCAAGGCG TGTTGAGCGT GGATTCCGCG 301 ATGCCGATGG TGCTGCATCT GCTGGCGCCG CTGGCAGCAA AATTCAACGA 351 ACGCTATCCG CATATCCGAC TTTCGCTCGT TTCTTCCGAA GGCTATATCA 401 ATCTGATTGA ACGCAAAGTC GATATTGCCT TACGGGCCGG AGAATTGGAC 451 GATTCCGGGC TGCGTGCACG CCATCTGTTT GACAGCCGCT TCCGCGTAAT 501 CGCCAGTCCT GAATACCTGG CAAAACACGG CACGCCGCAA TCTACAGAAG 551 AGCTTGCCGG CCACCAATGT TTAGGCTTCA CCGAACCCGG TTCTCTAAAT 601 ACATGGGCGG TTTTAGATGC GCAGGGAAAT CCCTATAAGA TTTCACCGCA 651 CTTTACCGCC AGCAGCGGTG AAATCTTACG CTCGTTGTGC CTTTCAGGTT 701 GCGGTATTGT TTGCTTATCA GATTTTTTGG TTGACAACGA CATCGCTGAA 751 GGAAAGTTAA TTCCCCTGCT CGCCGAACAA ACCTCCGATA AAACACACCC 801 CTTTAATGCT GTTTATTACA GCGATAAAGC CGTCAATCTC CGCTTACGCG 851 TATTTTTGGA TTTTTTAGTG GAGGAACTGG GAAACAATCT CTGTGGATAA This corresponds to the amino acid sequence <SEQ ID 658; ORF 158>: ml58.pep 1 MKTNSEELTV FVQWESGSF SRAAEQLAMA NSAVSRIVKR LEEKLGVNLL 51 NRTTRQLSLT EEGAQYFRRA QRILQEMAAA ETEMLAVHEI PQGVLSVDSA 101 MPMVLHLLAP LAAKFNERYP HIRLSLVSSE GYINLIERKV DIALRAGELD 151 DSGLRARHLF DSRFRVIASP EYLAKHGTPQ STEELAGHQC LGFTEPGSLN 201 TWAVLDAQGN PYKISPHFTA SSGEILRSLC LSGCGIVCLS DFLVDNDIAE 251 GKLIPLLAEQ TSDKTHPFNA VYYSDKAVNL RLRVFLDFLV EELGNNLCG* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N. gonorrhoeae ml58 / gl58 94.3% identity in 297 aa overlap 10 20 30 40 50 60 ml58.pep MKTΝSEELTVFVQ ESGSFSRAAEQLAMAΝSAVSRIVKRLEEKLGVΝLLΝRTTRQLSLT I II II II I I I II I I I I I II I I II I I I I I I I II I I I I I II II I I II I I II I I II I II : I I gl58 MKTΝSEELTVFVQWESGSFSRAAEQLEMAΝSAVSRIVKRLEEKLGVΝLLΝRTTRQLΝLT 10 20 30 40 50 60 70 80 90 100 110 120 ml58.pep EEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLSVDSAMPMVLHLLAPLAAKFΝERYP I II II II II I II I I I I I II I I II I I I I II: II I I I I I II II II I II I II II II I II II I gl58 EEGAQYFRRAQRILQEMAAAETEMLAVHEVPQGVLRVDΞAMPMVLHLLAPLAAKFΝERYP 70 80 90 100 110 120 130 140 150 160 170 180 ml58. pep HIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLFDSRFRVIASPEYLAKHGTPQ I II I II II II II I I II I II II I II II II II I I II II II II II: II I : I II II II II II II gl58 HIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLFDSHFRWASPEYLAKHGTPQ 130 140 150 160 170 180 190 200 210 220 230 240 ml58.pep STEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHFTASSGEILRSLCLSGCGIVCLS I : I: I I: II II II I I II I II II I II I II II I II II I I II I I II I II II I II I: I I I : II I gl58 SAEDLANHQCLGFTEPGSLNTWAVLDAQGNPYKISPHFTASSGEILRSLCLSSCGIACLS 190 200 210 220 230 240 250 260 270 280 290 300 ml58. pep DFLVDNDIAEGKLIPLLAEQTSDKTHPFNAVYYSDKAVNLRLRVFLDFLVEELGNNLCGX II I II II I: II II II I: II I II: I I I I II I II II II I I II I I I II II II I : I II: I : gl58 DFLVDNDITEGKLIPLFAEQTSNKTHPFNAVYYSDKAVNLRLRVFLDFLVKELGKNMNRT 250 260 270 280 290 300 gl58 NTKX The following partial DNA sequence was identified in N meningitidis <SEQ ID 659>: al58. seq 1 ATGAAAACCA ATTCAGAAGA ACTGACCGTA TTTGTTCAAG TGGTGGAAAG 51 CGGCAGCTTC AGCCGTGCGG CGGAGCAGTT GGCGATGGCA AATTCTGCCG 101 TAAGCCGCAT CGTCAAACGG CTGGAGGAAA AGTTGGGTGT GAACCTGCTC 151 AACCGCACCA CGCGGCAACT CAGTCTGACG GAAGAAGGCG CGCAATATTT 201 CCGCCGCGCG CAGAGAATCC TGCAAGAAAT GGCAGCGGCG GAAACCGAAA 251 TGCTGGCAGT GCACGAAATA CCGCAAGGCG TGTTGCGCGT GGATTCCGCG 301 ATGCCGATGG TGCTGCATCT GCTGGCGCCG CTGGCAGCAA AATTCAACGA 351 ACGCTATCCG CATATCCGAC TTTCGCTCGT TTCTTCCGAA GGCTATATCA 401 ATCTGATTGA ACGCAAAGTC GATATTGCCT TACGGGCCGG AGAATTGGAC 451 GATTCCGGGC TGCGTGCACG CCATCTGTTT GACAGCCGCT TCCGCGTAAT 501 CGCCAGTCCT GAATACCTGG CAAAACACGG CACGCCGCAA TCTACAGAAG 551 AGCTTGCCGG CCACCAATGT TTAGGCTTCA CCGAACCCGG TTCTCTAAAT 601 ACATGGGCGG TTTTAGATGC GCAGGGAAAT CCCTATAAGA TTTCACCGCA 651 CTTTACCGCC AGCAGCGGTG AAATCTTACG CTCGTTGTGC CTTTCAGGTT 701 GCGGTATTGC TTGCTTATCA GATTTTTTGG TTGACAACGA CATCGCTGAA 751 GGAAAGTTAA TTCCCCTGCT CGCCGAACAA ACCTCCAATA AAACGCACCC 801 CTTTAATGCT GTTTATTACA GCGATAAAGC CGTCAACCTC CGCTTACGCG 851 TATTTTTGGA TTTTTTAGTG GAGGAACTGG GAAACAATCT CTGTGGATAA This corresponds to the amino acid sequence <SEQ LD 660; ORF 158. a>: al58. .pep 1 MKTNSEELTV FVQWESGSF SRAAEQLAMA NSAVSRIVKR LEEKLGVNLL 51 NRTTRQLSLT EEGAQYFRRA QRILQEMAAA ETEMLAVHEI PQGVLRVDSA 101 MPMVLHLLAP LAAKFNERYP HIRLSLVSSE GYINLIERKV DIALRAGELD 151 DSGLRARHLF DSRFRVIASP EYLAKHGTPQ STEELAGHQC LGFTEPGSLN 201 TWAVLDAQGN PYKISPHFTA SSGEILRSLC LSGCGIACLS DFLVDNDIAE 251 GKLIPLLAEQ TSNKTHPFNA VYYSDKAVNL RLRVFLDFLV EELGNNLCG* ml58/al58 1 99.0% identity in 299 aa overlap 10 20 30 40 50 60 ml58. .pep MKTNSEELTVFVQWESGSFSRAAEQLAMANSAVSRIVKRLEEKLGVNLLNRTTRQLSLT I I II I II II II II II I I I I II I I II I II II I II II II I I I II I I II II I II II I I I II I I al58 MKTNSEELTVFVQWESGSFSRAAEQLAMANSAVSRIVKRLEEKLGVNLLNRTTRQLSLT 10 20 30 40 50 60 70 80 90 100 110 120 ml58. pep EEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLSVDSAMPMVLHLLAPLAAKFNERYP II I II II I II II II II I I I I I II I I I I II I II I I I I I I I II I I I II I II II II II I I I I al58 EEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMPMVLHLLAPLAAKFNERYP 70 80 90 100 110 120 130 140 150 160 170 180 ml58. pep HIRLSLVSSEGYINLIERKVDIALFAGELDDSGLRARHLFDSRFRVIASPEYLAKHGTPQ II II II II II I II I I I I II II I I I I I II I II I I II I I I II I II II II II II II II II I II al58 HIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLFDSRFRVIASPEYLAKHGTPQ 130 140 150 160 170 180 190 200 210 220 230 240 ml58.pep STEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHFTASSGEILRSLCLSGCGIVCLS I I II II I I I I II II II I I II II II I I I II I I II II II II II I I I I II I I I I II II I : I II al58 STEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHFTASSGEILRSLCLSGCGIACLS 190 200 210 220 230 240 250 260 270 280 290 300 ml58. pep DFLVDNDIAEGKLIPLLAEQTSDKTHPFNAVYYSDKAVNLRLRVFLDFLVEELGNNLCGX II I II I II II I II II II II II I: I II II I II I II II II II I II I II II II I I II I II II I al58 DFLVDNDIAEGKLIPLLAEQTSNKTHPFNAVYYSDKAVNLRLRVFLDFLVEELGNNLCGX 250 260 270 280 290 300 The following partial DNA sequence was identified in N gonorrhoeae <SEQ LD 661>: gl60. seq 1 ATGGAcattc tgGACAAact ggtcgatCTC GCccaATTGA CGGGCAGTGC 51 GGATGTGCAG TgcctTTTGG GCGGACAATG gcATGaaacc TTGCAACGCG 101 AAGGGCTGGT ACACATTGTT ACGGCGGGCA GCGGTTATCT CTGCATCGAC 151 GGCGAAACTT CCCCGCGTCC GGTCGGCACG GGCGATATTG TATTTTTCCC 201 GCGCGGCTTG GGTCATGTGT TGAGCCACGA CGGAAAATAC GGAGAAAGTT 251 TACAACCGGA CATACGACAA AACGGCACAT TTATGGTCAA ACAGTGCGGC 301 AACGGGCTGG ATATGAGCCT GTTTTGCGCC CGTTTCCGCT ACGACACCCA 351 CGCCGATTTG ATGAACGGGC TGCCGGAAAC CGTTTTTCTG AACATTGCCC 401 ATCCAAGTTT GCAGTATGTG GTTTCAATGC TGCAACTGGA AAGCGAAAAA 451 CCTTTGACGG GGACGGTTTC CGTGGTCAAC GCATTACCGT CCGTCCTGCT 501 GGTGCTTATC CTGCGCGCCT ATCTCGAACA GGATAAGGAT GTCGAACTCT 551 CGGGCGTATT GAAAGGTTGG CAGGACAAAC GTTTGGGACA TTTGATCCAA 601 AAGGTGATAG ACAAACCGGA AGACGAATGG AATATTGACA AAATGGTTGC 651 CGCCGCCAAT ATGTCGCGCG CGCAACTGAT GCGCCGCTTC AAAAGCCAAG 701 TCGGACTCAG CCCGCACGCC TTTGTGAACC ATATCCGCCT GCAAAAAGGC 751 GCATTGCTGC TGAAGAAAAC CCCGGATTCG GTTTTGGAGG TCGCGCTGTC 801 GGTGGGCTTT CAGTCGGAAA CGCATTTCGG CAAGGCGTTC AAACGGCAAT 851 ATCACGTTTC GCCGGGGCAA TACCGGAAAG AAGGCGGGCA AAAATAA This corresponds to the amino acid sequence < SEQ ID 662; ORF 160.ng > : glδO.pep 1 MDILDKLVDL AQLTGSADVQ CLLGGQWHET LQREGLVHIV TAGSGYLCID 51 GETSPRPVGT GDIVFFPRGL GHVLSHDGKY GESLQPDIRQ ΝGTFMVKQCG 101 ΝGLDMSLFCA RFRYDTHADL MΝGLPETVFL ΝIAHPSLQYV VSMLQLESEK 151 PLTGTVSVVΝ ALPSVLLVLI LRAYLEQDKD VELSGVLKGW QDKRLGHLIQ 201 KVIDKPEDEW ΝIDKMVAAAΝ MSRAQLMRRF KSQVGLSPHA FVΝHIRLQKG 251 ALLLKKTPDS VLEVALSVGF QSETHFGKAF KRQYHVSPGQ YRKEGGQK* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 663>: ml60. seq 1 ATGGACATTC TGGACAAACT GGTCGATTTC GCCCAATTGA CGGGCAGTGT 51 GGATGTGCAG TGCCTTTTGG GCGGACAATG GTCGGTACGG CATGAAACCT 101 TGCAACGCGA AGGATTGGTA CACATTGTTA CATCGGGCAG CGGCTATCTC 151 TGCATCGACG GCGAAACTTC CCCGCGTCCG GTCAGTACAG GGGATATTGT 201 ATTTTTCCCG CGCGGCTTGG GTCATGTGTT GAGCCACGAC GGAAAATGCG 251 GAGAAAGTTT ACAACCGGAT ATGCGGCAGC ACGGTGCGTT TACGGTCAAG 301 CAGTGCGGCA ACGGACAGGA TATGAGCCTG TTTTGCGCCC GTTTCCGCTA 351 CGACACCCAC GCCGATTTGA TGAACGGGCT GCCTGAAACC GTTTTTCTGA 401 ACATTGCCCA TCCGAGTTTA CAGTATGTGG TTTCAATGCT GCAACTGGAA 451 AGCAAAAAAC CTTTGACGGG GACGGTTTCC ATGGTCAACG CATTGTCGTC 501 CGTCCTGCTG GTGCTTATCC TGCGCGCCTA TCTCGAACAG GATAAGGATG 551 TCGAACTCTC GGGCGTATTG AAAGGTTGGC AGGACAAACG TTTGGGACAT 601 TTAATCCAAA AGGTGATAGA CAAACCGGAA GACGAATGGA ATGTCGACAA 651 AATGGTGGCG GCTGCCAATA TGTCGCGCGC GCAACTGATG CGCCGTTTCA 701 AAAGCCGGGT CGGACTCAGC CCGCACGCCT TTGTGAACCA TATCCGCCTG 751 CAAAAAGGCG CGTTGCTGCT GAAAAAAAAC CCGGATTCGG TTTTGTCGGT 801 CGCACTGTCG GTAGGCTTTC AGTCGGAAAC GCACTTCGGC AAGGCGTTCA 851 AACGGCAATA TCACGTTTCG CCGGGTCAAT ACCGGAAAGA AggCGGGCAA 901 AAATAA This corresponds to the amino acid sequence <SEQ ID 664; ORF 160>: ml 60.pep 1 MDILDKLVDF AQLTGSVDVQ CLLGGQWSVR HETLQREGLV HIVTSGSGYL 51 CIDGETSPRP VSTGDIVFFP RGLGHVLSHD GKCGESLQPD MRQHGAFTVK 101 QCGNGQDMSL FCARFRYDTH ADLMNGLPET VFLNIAHPSL QYWSMLQLE 151 SKKPLTGTVS MVNALSSVLL VLILRAYLEQ DKDVELSGVL KGWQDKRLGH 201 LIQKVIDKPE DEWNVDKMVA AANMSRAQLM RRFKSRVGLS PHAFVNHIRL 251 QKGALLLKKN PDSVLSVALS VGFQSETHFG KAFKRQYHVS PGQYRKEGGQ 301 K* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ml60 / gl60 93.4% identity in 301 aa overlap 10 20 30 40 50 60 ml 60. • pep MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVTSGSGYLCIDGETSPRP II I II I I I I : I II II I : I I II I II I II II I II I I I II I I I I: II I II II I I II I I II gl60 MDILDKLVDLAQLTGSADVQCLLGGQW HETLQREGLVHIVTAGSGYLCIDGETSPRP 10 20 30 40 50 70 80 90 100 110 120 ml 60, • pep VSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQCGΝGQDMSLFCARFRYDTH I : I I II I I I I II II I I I I I I II I II I I I I : I I : I : I lllllll I II I II I I II I II I gl60 VGTGDIVFFPRGLGHVLSHDGKYGESLQPDIRQΝGTFMVKQCGΝGLDMSLFCARFRYDTH 60 70 80 90 100 110 130 140 150 160 170 180 ml60. pep ADLMΝGLPETVFLΝIAHPSLQYWSMLQLESKKPLTGTVSMVΝALSSVLLVLILRAYLEQ II I II I I I I II I I II II I I II II II I I I II I : I I I II I I I : I I I I II I II I I II I I II I gl60 ADLMΝGLPETVFLΝIAHPSLQYWSMLQLESEKPLTGTVSWΝALPSVLLVLILRAYLEQ 120 130 140 150 160 170 190 200 210 220 230 240 ml60. pep DKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWΝVDKMVAAAΝMSRAQLMRRFKSRVGLS I I I I I II I II II II I I I I II II I I I I I I I I I II I : I II II I II II II I II I II I I: I I II gl60 DKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWΝIDKMVAAAΝMSRAQLMRRFKSQVGLS 180 190 200 210 220 230 250 260 270 280 290 300 ml60. pep PHAFVΝHIRLQKGALLLKKΝPDSVLSVALSVGFQSETHFGKAFKRQYHVSPGQYRKEGGQ I I I I II I I II II II I I I I I : II I I I I II I I II I I I I I II I I I II II I II I II II I II I I gl60 PHAFVΝHIRLQKGALLLKKTPDSVLEVALSVGFQSETHFGKAFKRQYHVSPGQYRKEGGQ 240 250 260 270 280 290 ml60. pep KX I I gl60 KX The following partial DΝA sequence was identified in N meningitidis <SEQ LD 665>: al60. seq 1 ATGGACATTC TGGACAAACT GGTCGATTTC GCCCAATTGA CGGGCAGTGT 51 GGATGTGCAG TGCCTTTTGG GCGGACAATG GTCGGTACGG CATGAAACCT 101 TGCAACGCGA AGGATTGGTA CACATTGTTA CATCGGGCAG CGGCTATCTC 151 TGCATCGACG GCGAAACTTC CCCGCGTCCG GTCAGTACAG GGGATATTGT 201 ATTTTTCCCG CGCGGCTTGG GTCATGTGTT GAGCCACGAC GGAAAATGCG 251 GAGAAAGTTT ACAACCGGAT ATGCGGCAGC ACGGTGCGTT TACGGTCAAG 301 CAGTGCGGCA ACGGACAGGA TATGAGCCTG TTTTGCGCCC GTTTCCGCTA 351 CGACACCCAC GCCGATTTGA TGAACGGGCT GCCTGAAACC GTTTTTCTGA 401 ACATTGCCCA TCCGAGTTTA CAGTATGTGG TTTCAATGCT GCAACTGGAA 451 AGCAAAAAAC CTTTGACGGG GACGGTTTCC ATGGTCAACG CATTGTCGTC 501 CGTCCTGCTG GTGCTTATCC TGCGCGCCTA TCTCGAACAG GATAAGGATG 551 TCGAACTCTC GGGCGTATTG AAAGGTTGGC AGGACAAACG TTTGGGACAT 601 TTAATCCAAA AGGTGATAGA CAAACCGGAA GACGAATGGA ATGTCGACAA 651 AATGGTGGCG GCTGCCAATA TGTCGCGCGC GCAACTGATG CGCCGTTTCA 701 AAAGCCGGGT CGGACTCAGC CCGCACGCCT TTGTGAACCA TATCCGCCTG 751 CAAAAAGGCG CGTTGCTGCT GAAAAAAAAC CCGGATTCGG TTTTGTCGGT 801 CGCACTGTCG GTAGGCTTTC AGTCGGAAAC GCACTTCGGC AAGGCGTTCA 851 AACGGCAATA TCACGTTTCG CCGGGTCAAT ACCGGAAAGA AGGCGGGCAA 901 AAATAA This corresponds to the amino acid sequence <SEQ ID 666; ORF 160.a>: al60.pep 1 MDILDKLVDF AQLTGSVDVQ CLLGGQWSVR HETLQREGLV HIVTSGSGYL 51 CIDGETSPRP VSTGDIVFFP RGLGHVLSHD GKCGESLQPD MRQHGAFTVK 101 QCGNGQDMSL FCARFRYDTH ADLMNGLPET VFLNIAHPSL QYWSMLQLE 151 SKKPLTGTVS MVNALSSVLL VLILRAYLEQ DKDVELSGVL KGWQDKRLGH 201 LIQKVIDKPE DEWNVDKMVA AANMSRAQLM RRFKSRVGLS PHAFVNHIRL 251 QKGALLLKKN PDSVLSVALS VGFQSETHFG KAFKRQYHVS PGQYRKEGGQ 301 K* ml60/al60 100.0% identity in 301 aa overlap 10 20 30 40 50 60 ml 60. pep MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVTSGSGYLCIDGETSPRP II II I I I I I II II I I I I I I I I I II II I II II I I II II I II I I I II II I I II I II I II I I I al60 MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVTSGSGYLCIDGETSPRP 10 20 30 40 50 60 70 80 90 100 110 120 ml 60. pep VSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQCGNGQDMSLFCARFRYDTH II II II I I I I I I II II I I II I II I I II I I I I I I I I II I I I II I I I I II I I I I I II I I II I al60 VSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQCGNGQDMSLFCARFRYDTH 70 80 90 100 110 120 130 140 150 160 170 180 ml60. pep ADLMNGLPETVFLNIAHPSLQYWSMLQLESKKPLTGTVSMVNALSSVLLVLILRAYLEQ I I I I II II I I II I II II II II II I II II I II I II II I I II I I I I I II I I II I I I I I II II al60 ADLMNGLPETVFLNIAHPSLQYWSMLQLESKKPLTGTVSMVNALSSVLLVLILRAYLEQ 130 140 150 160 170 180 190 200 210 220 230 240 ml60. pep DKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKMVAAANMSRAQLMRRFKSRVGLS I I I I I II II II II I I I I I II II I I I II I I I II I I I II I I II I I I II II I I I II I II I I I I al60 DKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKMVAAANMSRAQLMRRFKSRVGLS 190 200 210 220 230 240 250 260 270 280 290 300 ml60. pep PHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSETHFGKAFKRQYHVSPGQYRKEGGQ II I I II II II II II I II II II I I I II I II II II II I I I II I I I II II I I II I II I I II II al60 PHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSETHFGKAFKRQYHVSPGQYRKEGGQ 250 260 270 280 290 300 ml60.pep KX I I al60 KX The following partial DNA sequence was identified in N gonorrhoeae <SEQ ED 667>: gl61. seq 1 ATGGATACCG CAAAAAAAGA CATTTTAGGA TCGGGCTGGA TGCTGGTGGC 51 GGCGGCCTGC TTCACCGTTA TGAACGTATT GATTAAAGAG GCATCGGCAA 101 AATTTGCCCT CGGCAGCGGC GAATTGGTCT TTTGGCGCAT GCTGTTTTCA 151 ACCGTTACGC TCGGTGCTGC CGCCGTATTG CGGCGCGACA CCTTCCGCAC 201 GCCCCATTGG AAAAACCACT TAAACCGCAG TATGGTCGGG ACGGGGGCGA 251 TGCTGCTGCT GTTTTACGCG GTAACGCATC TGCCTTTGAC AACCGGCGTT 301 ACCCTGAGTT ACACCTCGTC GATTTTTttg GCGGTATTTT CCTTCCTGAT 351 TTTGAAAGAA CGGATTTCCG TTTACACGCA GGCGGTGCTG CTCCTTGGTT 401 TTGCCGGCGT GGTATTGCTG CTTAATCCCT CGTTCCGCAG CGGTCAGGAA 451 CCGGCGGCAC TCGCCGGGCT GGCGGGCGGC GCGATGTCCG GCTGGGCGTA 501 TTTGAAAGTG CGCGAACTGT CTTTGGCGGG CGAACCCGGC TGGCGCGTCG 551 TGTTTTACCT TTCCGCAACC GGCGTGGCGA TGTCGTCggt ttgggcgacg 601 Ctgaccggct ggCACAcccT GTCCTTTcca tcggcagttt ATCtgtCGGG 651 CATCGGCGTG tccgcgCtgA TTGCCCAaCT GtcgatgAcg cGCGcctaca 701 aaGTCGGCGA CAAATTCACG GTTGCCTCGC tttcctaTAt gaccgtcGTC 751 TTTTCCGCCC TGTCTGCCGC ATTTTTTCTg ggcgaagagc ttttctggCA 801 GGAAATACTC GGTATGTGCA TCATTATcct CAGCGGCATT TTGAGCAGCA 851 TCCGCCCCAT TGCCTTCAAA CAGCGGCTGC AAGCCCTCTT CCGCCAAAGA 901 TAA This corresponds to the amino acid sequence <SEQ ID 668; ORF 161.ng>: 1 MDTAKKDILG SGWMLVAAAC FTVMΝVLIKE ASAKFALGSG ELVFWRMLFS 51 TVTLGAAAVL RRDTFRTPHW KΝHLΝRSMVG TGAMLLLFYA VTHLPLTTGV 101 TLSYTSSIFL AVFSFLILKE RISVYTQAVL LLGFAGWLL LΝPSFRSGQE 151 PAALAGLAGG AMSGWAYLKV RELSLAGEPG WRVVFYLSAT GVAMSSVWAT 201 LTGWHTLSFP SAVYLSGIGV SALIAQLSMT RAYKVGDKFT VASLSYMTW 251 FSALSAAFFL GEELFWQEIL GMCIIILSGI LSSIRPIAFK QRLQALFRQR 301 * The following partial DΝA sequence was identified in N meningitidis <SEQ ID 669>: ml61. seq 1 ATGGATACCG CAAAAAAAGA CATTTTAGGA TCGGGCTGGA TGCTGGTGGC 51 GGCGGCCTGC TTTACCATTA TGAACGTATT GATTAAAGAG GCATCGGCAA 101 AATTTGCCCT CGGCAGCGGC GAATTGGTCT TTTGGCGCAT GCTGTTTTCA 151 ACCGTTGCGC TCGGGGCTGC CGCCGTATTG CGTCGGGACA mCTTCCGCAC 201 GCCCCATTGG AAAAACCACT TAAACCGCAG TATGGTCGGG ACGGGGGCGA 251 TGCTGCTGCT GTTTTACGCG GTAACGCATC TGCCTTTGGC CACTGGCGTT 301 ACCCTGAGTT ACACCTCGTC GATTTTTTTG GCGGTATTTT CCTTCCTGAT 351 TTTGAAAGAA CGGATTTCCG TTTACACGCA GGCGGTGCTG CTCCTTGGTT 401 TTGCCGGCGT GGTATTGCTG CTTAATCCCT CGTTCCGCAG CGGTCAGGAA 451 ACGGCGGCAC TCGCCGGGCT GGCGGGCGGC GCGATGTCCG GCTGGGCGTA 501 TTTGAAAGTG CGCGAACTGT CTTTGGCGGG CGAACCCGGC TGGCGCGTCG 551 TGTTTTACCT TTCCGTGACA GGTGTGGCGA TGTCGTCGGT TTGGGCGACG 601 CTGACCGGCT GGCACACCCT GTCCTTTCCA TCGGCAGTTT ATCTGTCGTG 651 CATCGGCGTG TCCGCGCTGA TTGCCCAACT GTCGATGACG CGCGCCTACA 701 AAGTCGGCGA CAAATTCACG GTTGCCTCGC TTTCCTATAT GACCGTCGTT 751 TTTTCCGCTC TGTCTGCCGC ATTTTTTCTG GGCGAAGAGC TTTTCTGGCA 801 GGAAATACTC GGTATGTGCA TCATCATCCT CAGCGGTATT TTGAGCAGCA 851 TCCGCCCCAC TGCCTTCAAA CAGCGGCTGC AATCCCTGTT CCGCCAAAGA 901 TAA This corresponds to the amino acid sequence <SEQ ID 670; ORF 161>: ml 61.pep 1 MDTAKKDILG SGWMLVAAAC FTIMΝVLIKE ASAKFALGSG ELVFWRMLFS 51 TVALGAAAVL RRDXFRTPHW KΝHLΝRSMVG TGAMLLLFYA VTHLPLATGV 101 TLSYTSSIFL AVFSFLILKE RISVYTQAVL LLGFAGWLL LΝPSFRSGQE 151 TAALAGLAGG AMSGWAYLKV RELSLAGEPG WRWFYLSVT GVAMSSVWAT 201 LTGWHTLSFP SAVYLSCIGV SALIAQLSMT RAYKVGDKFT VASLSYMTW 251 FSALSAAFFL GEELFWQEIL GMCIIILSGI LSSIRPTAFK QRLQSLFRQR 301 * Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ml61 / gl61 97.0% identity in 300 aa overlap 10 20 30 40 50 60 ml61. pep MDTAKKDILGSGWMLVAAACFTIMΝVLIKEASAKFALGSGELVFWRMLFSTVALGAAAVL I I I I I I I I I I I II I II I I II I I : I I I II I II II I II II I II II I II II I II I: II II I I I gl 61 MDTAKKDILGSGWMLVAAACFTVMΝVLIKEASAKFALGSGELVFWRMLFSTVTLGAAAVL 10 20 30 40 50 60 70 80 90 100 110 120 ml61. pep RRDXFRTPHWKΝHLΝRSMVGTGAMLLLFYAVTHLPLATGVTLSYTSSIFLAVFSFLILKE II I: I II II II I II I II II II I I I I I I I II I II II I : I I I I I II I I I II I II II II I I II gl61 RRDTFRTPHWKΝHLΝRSMVGTGAMLLLFYAVTHLPLTTGVTLSYTSSIFLAVFSFLILKE 70 80 90 100 110 120 130 140 150 160 170 180 ml 61. pep RISVYTQAVLLLGFAGVVLLLΝPSFRSGQETAALAGLAGGAMSGWAYLKVRELSLAGEPG I I I I II II I II II I I I II II I II II I I II I II I I I I II I I II I II II I II II II II I I I gl61 RISVYTQAVLLLGFAGWLLLΝPSFRSGQEPAALAGLAGGAMSGWAYLKVRELSLAGEPG 130 140 150 160 170 180 190 200 210 220 230 240 ml61. pep WRWFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCIGVSALIAQLSMTRAYKVGDKFT I II I I I I I : I II I II I I I II II II I II I I II I II II I I II I I I II I I I I I II I I II I I I gl61 WRWFYLSATGVAMSSVWATLTGWHTLSFPSAVYLSGIGVSALIAQLSMTRAYKVGDKFT 190 200 210 220 230 240 250 260 270 280 290 300 mlβl.pep VASLSYMTWFSALSAAFFLGEELFWQEILGMCIIILSGILSSIRPTAFKQRLQSLFRQR II II I II II II I II I II II I II II I I I I II I I I II II I I II I II II I II I I I I : II I I I gl61 VAΞLSYMTWFSALSAAFFLGEELFWQEILGMCIIILSGILSSIRPIAFKQRLQALFRQR 250 260 270 280 290 300 ml 61. pep X I gl61 X The following partial DΝA sequence was identified in N meningitidis <SEQ ID 671>: al61. seq 1 ATGGATACCG CAAAAAAAGA CATTTTAGGA TCGGGCTGGA TGCTGGTGGC 51 GGCGGCCTGC TTTACCATTA TGAACGTATT GATTAAAGAG GCATCGGCAA 101 AATTTGCCCT CGGCAGCGGC GAATTGGTCT TTTGGCGCAT GCTGTTTTCA 151 ACCGTTGCGC TCGGGGCTGC CGCCGTATTG CGTCGGGACA CCTTCCGCAC 201 GCCCCATTGG AAAAACCACT TAAACCGCAG TATGGTCGGG ACGGGGGCGA 251 TGCTGCTGCT GTTTTACGCG GTAACGCATC TGCCTTTGGC CACCGGCGTT 301 ACCCTGAGTT ACACCTCGTC GATTTTTTTG GCGGTATTTT CCTTCCTGAT 351 TTTGAAAGAA CGGATTTCCG TTTACACGCA GGCGGTGCTG CTCCTTGGTT 401 TTGCCGGCGT GGTATTGCTG CTTAATCCCT CGTTCCGCAG CGGTCAGGAA 451 ACGGCGGCAC TCGCCGGGCT GGCGGGCGGC GCGATGTCCG GCTGGGCGTA 501 TTTGAAAGTG CGCGAACTGT CTTTGGCGGG CGAACCCGGC TGGCGCGTCG 551 TGTTTTACCT TTCCGTGACA GGTGTGGCGA TGTCATCGGT TTGGGCGACG 601 CTGACCGGCT GGCACACCCT GTCCTTTCCA TCGGCAGTTT ATCTGTCGTG 651 CATCGGCGTG TCCGCGCTGA TTGCCCAACT GTCGATGACG CGCGCCTACA 701 AAGTCGGCGA CAAATTCACG GTTGCCTCGC TTTCCTATAT GACCGTCGTT 751 TTTTCCGCTC TGTCTGCCGC ATTTTTTCTG GCCGAAGAGC TTTTCTGGCA 801 GGAAATACTC GGTATGTGCA TCATCATCCT CAGCGGTATT TTGAGCAGCA 851 TCCGCCCCAC TGCCTTCAAA CAGCGGCTGC AATCCCTGTT CCGCCAAAGA 901 TAA This corresponds to the amino acid sequence <SEQ ED 672; ORF 161. a>: al61.pep 1 MDTAKKDILG SGWMLVAAAC FTIMNVLIKE ASAKFALGSG ELVFWRMLFS 51 TVALGAAAVL RRDTFRTPHW KNHLNRSMVG TGAMLLLFYA VTHLPLATGV 101 TLSYTSSIFL AVFSFLILKE RISVYTQAVL LLGFAGWLL LNPSFRSGQE 151 TAALAGLAGG AMSGWAYLKV RELSLAGEPG WRWFYLSVT GVAMSSVWAT 201 LTGWHTLSFP SAVYLSCIGV SALIAQLSMT RAYKVGDKFT VASLSYMTW 251 FSALSAAFFL AEELFWQEIL GMCIIILSGI LSSIRPTAFK QRLQSLFRQR 301 * ml61/al61 99.3% identity in 300 aa overlap 10 20 30 40 50 60 ml 61. pep MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFALGSGELVFWRMLFSTVALGAAAVL II II I II I I II I II I I I I II II II II I I II II I II I II I II I I II I II I II II II II I M al 61 MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFALGSGELVFWRMLFSTVALGAAAVL 10 20 30 40 50 60 70 80 90 100 110 120 ml 61. pep RRDXFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTLSYTSSIFLAVFSFLILKE II I : I I I I I II I II II I I II II II II II II II I II I II I II I I II I II I II II II II I II al61 RRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTLSYTSSIFLAVFSFLILKE 70 80 90 100 110 120 130 140 150 160 170 180 ml61. pep RISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGGAMSGWAYLKVRELSLAGEPG II I II II I I II I II I I I II I II I II II I II II I I I I II I II II II I II I I I II II II I II al61 RISVYTQAVLLLGFAGWLLLNPSFRSGQETAALAGLAGGAMSGWAYLKVRELSLAGEPG 130 140 150 160 170 180 190 200 210 220 230 240 ml61. pep WRVVFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCIGVSALIAQLSMTRAYKVGDKFT II I II II II II II I I II II II I I I II II II II II II I II II II II II II I II I I I I II I I al61 WRWFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCIGVSALIAQLSMTRAYKVGDKFT 190 200 210 220 230 240 250 260 270 280 290 300 ml 61. pep VASLSYMTWFSALSAAFFLGEELFWQEILGMCIIILSGILSSIRPTAFKQRLQSLFRQR I II II II I I I I II II I II I I : I II I II II I I II II II I II II II I I II I II II II II II I al61 VASLSYMTWFSALSAAFFLAEELFWQEILGMCIIILSGILΞSIRPTAFKQRLQSLFRQR 250 260 270 280 290 300 ml61.pep X I al61 x The following partial DNA sequence was identified in N gonorrhoeae <SEQ LD 673>: gl63. seq 1 ATGGTTATTT TGACGACTTT GTTTTTTGTG TGTGTTTTGG TGGTATTGGT 51 TTTAACCGTG CCGGATCAGG TGCAGATGTG gctCGACCGG GCAAAAGAAG 101 TCATTTTTAC CGAGTTCAGC TGGTTTTATG TTTTAACGTT TTCCATTTTt 151 ctgGGTTTtc tgctGATACT CTCGGTCAGC GGTTTGGGAA ACATcagGCT 201 AGGACGGGAT GAAGATGTGC CGGAATTCGG CTTCCTGTCG TGGCTGGCGA 251 TGCTGTTTGC GGCCGGGATG GGCGTGGGCC TGATGTTTTT CGGCGTGGCA 301 GAGCCGTTGA TGCATTATTT TTCGGACATT ACGGTCGGCG CGCCGGAACA 351 CAGGCAGCAG CAGGCATTGC TGCACACGGT GTTCCATTGG GGCGTTCACG 401 CCTGGTCGGT GTACGGTACG ATTGCATTGG CTTTGGCTTA TTTCGGTTTC 451 CGCTACAAAC TGCCGCTTGC CCTGCGTTCT TGTTTTTACC CCCTGTTGAA 501 AGAAAAAATT TCCGGAAGGT TCGGCGATGC CATTGATATT ATGGCGTTGC 551 TTGCTACTTT TTTCGGCATC ATCACCACAT TGGGGTTCGG GGCTTCGCAA 601 CTGGGCGCCG GATTGCAGGA AATGGGCTGG ATTGCCGAAA ACAGCTTCGG 651 CGTGCAGGTC TTGATTATCG CCGCCGTAAT GTCCCTCGCC GTCGTTTCGG 701 CAATATCCGG CGTGGGGAAG GGCGTGAAGG TGTTGAGCGA GTTGAACCTG 751 GGCCTTGCGT TTTTGCTGCT GTTTTTTGTT TTGGCGGCGG ACCCCACTGT 801 TTACCTGTTG TCGGCATTCG GCGACAACAT AGGGAACTAC CTCGGAAATC 851 TGGTGCGCCT CAGTTTGAAA ACTTATGCGT ACGAACGGGA ACACAAGCCG 901 TGGTTTGAAT CTTGGACGGT GCTTTATTGG GCGTGGTGGT GTTCTTGGgc 951 gcCGTTTGTG GGTTTGTTTA TCGCGCGCAT TTCAAAGGGg cgcaccatCc 1001 gcgagtttgt CTTCGGGGTT TTGCTCATCC CCGGCCTGTT CGGCGTTTTG 1051 TGGTTTACCG TCTTCGGCAA TACGGCGATT TGGCTGAATG ACGGGGTTGC 1101 GGGGGGAATG CTCGAAAAGA TGACCTCCTC TCCGGAAACG CTGCTTTTTA 1151 AATTCTTTAA TTACCTCCCC CTGCCCGAAC TGACGAGCAT CGTCAGCCTG 1201 CTGGTCATTT CCCTGTTTTT TGTAACTTCT GCCGACTCCG GGATTTATGT 1251 CCTGAACAAT ATTACCTCTC GGGACAAAGG CTTGAGCGCG CCACGGTGGC 1301 AGGCGGTTAT GTGGGGCGTG CTGatgtcTG CCGTTGCCGT TTTGCTGATG 1351 CGCTCGGGCG GACTCGGCAA CCTGCAGTCT ATGACCCTGA TTGTTTCCCT 1401 GCCGTTTGCC CTGCTGATGC TGATAATGTG TTTCAGCCTG TGGAAAGGCT 1451 TGAGTGCGGA TAAGAAATAT TTTGAGACCC GGGTCAACCC TACCAGTGTA 1501 TTTTGGACGG GCGGCAAGTG GAAAGAACGG CTGGTGCGGA TAATGAGCCA 1551 GACGCAGGAG CAGGATATTT TAAAATTCCT CAAACATACC GCATCGCCCG 1601 CTATGCACGA GTTGCAACGG GAGCTTTCGG AAGAATACGG CTTGAGCGTC 1651 CGGGTCGATA AGATGTTTCA TCAGGACGAG CCCGCAATCG AGTTCGTCAT 1701 TCGGAAAGAG ACGATGCGCG ATTTTATGTA CGGGATTAAG TCTGTCGGGC 1751 AGGATGTATC CGACCAGTTG ATTAACGACG GCAAGCTGCC GCATATCCGG 1801 CACCAGACAA CTTACAAACC CTACGCTTAT TTTTTCGACG GGCGCGTCGG 1851 GTACGATGTG CAGTATATGA ACAAGGACGA GCTGATTGCC GACATTTTGA 1901 AAAACTACGA ACGTTATTTG ATGTTGTTGG ATGATGTCGG TCAGGAACTG 1951 ATGGCGCACG AGCAGGTGGA ATTGGCAGAG TAA This corresponds to the amino acid sequence <SEQ ID 674; ORF 163.ng>: gl63.pep 1 MVILTTLFFV CVLWLVLTV PDQVQMWLDR AKEVIFTEFS WFYVLTFSIF 51 LGFLLILSVS GLGNIRLGRD EDVPEFGFLS WLAMLFAAGM GVGLMFFGVA 101 EPLMHYFSDI TVGAPEHRQQ QALLHTVFHW GVHAWSVYGT IALALAYFGF 151 RYKLPLALRS CFYPLLKEKI SGRFGDAIDI MALLATFFGI ITTLGFGASQ 201 LGAGLQEMGW IAENSFGVQV LIIAAVMSLA WSAISGVGK GVKVLSELNL 251 GLAFLLLFFV LAADPTVYLL SAFGDNIGNY LGNLVRLSLK TYAYEREHKP 301 WFESWTVLYW AWWCSWAPFV GLFIARISKG RTIREFVFGV LLIPGLFGVL 351 WFTVFGNTAI WLNDGVAGGM LEKMTSSPET LLFKFFNYLP LPELTSIVSL 401 LVISLFFVTS ADSGIYVLNN ITSRDKGLSA PRWQAVMWGV LMSAVAVLLM 451 RSGGLGNLQS MTLIVSLPFA LLMLIMCFSL WKGLSADKKY FETRVNPTSV 501 FWTGGKWKER LVRIMSQTQE QDILKFLKHT ASPAMHELQR ELSEEYGLSV 551 RVDKMFHQDE PAIEFVIRKE TMRDFMYGIK SVGQDVSDQL INDGKLPHIR 601 HQTTYKPYAY FFDGRVGYDV QYMNKDELIA DILKNYERYL MLLDDVGQEL 651 MAHEQVELAE * The following partial DNA sequence was identified in N meningitidis <SEQ ID 675>: ml 63. seq 1 ATGGTTATTT TGACGACTTT GTTTTTTGTG TGTGTTTTGG TGGTATTGGT 51 TTTAACCGTG CCGGATCAGG TGCAGATGTG GCTCGATCGG GCAAAAGAAG 101 TCATTTTTAC CGAGTTCAGC TGGTTTTATG TTTTAACGTT TTCCATTTTT 151 CTGGGTTTCC TGCTGATACT CTCGGTCAGC AGTTTGGGAA ACATCAGGCT 201 CGGACGGGAT GAAGATGTGC CGGAATTCGG CTTCCTGTCG TGGCTGGCGA 251 TGCTGTTTGC GGCCGGGATG GGCGTGGGTC TGATGTTTTT CGGCGTGGCA 301 GAGCCGTTGA TGCATTATTT TTCGGACATT ACGGCCGGCA CGCCGGAACA 351 CAGGCAGCAG CAGGCATTGC TGCACACGGT GTTCCATTGG GGCGTTCACG 401 CTTGGTCGGT GTACGGTACG ATTGCATTGG CTTTGGCTTA TTTCGGTTTC 451 CGCTACAAGC TGCCGCTTGC CCTGCGTTCT TGTTTTTACC CCCTGTTGAA 501 AGAAAAAATT TCCGGAAGGT TCGGCGATGC CATTGATATT ATGGCGTTGC 551 TTGCTACTTT TTTCGGCATC ATCACCACAT TGGGGTTCGG GGCTTCGCAA 601 CTGGGCGCCG GATTGCAGGA AATGGGCTGG ATTGCCGAAA ACAGCTTCAG 651 CGTGCAGGTT TTGATTATCG CCGCCGTCAT GTCCCTCGCC GTCGTTTCGG 701 CAATATCCGG CGTGGGGAAG GGCGTGAAGG TGTTGAGCGA GTTGAACCTG 751 GGCCTTGCGT TTTTGCTGCT GTTTTTTGTT TTGGCGGCGG GACCCACTGT 801 TTACCTGTTG TCGGCATTCG GCGACAACAT AGGGAACTAC CTCGGAAATC 851 TGGTGCGCCT CAGTTTTAAA ACTTATGCGT ACGAACGGGA ACACAAGCCG 901 TGGTTTGAAT CTTGGACGGT GCTTTATTGG GCGTGGTGGT GTTCTTGGGC 951 GCCGTTTGTG GGTTTGTTTA TCGCGCGCAT TTCAAAGGGG CGCACCATCC 1001 GCGAGTTTGT CTTCGGGGTT TTGCTCATCC CCGGCCTGTT CGGCGTTTTG 1051 TGGTTTACCG TCTTCGGCAA TACGGCGATT TGGCTGAATG ACGGGGTTGC 1101 GGGGGGAATG CTCGAAAAGA TGACCTCCTC TCCGGAAACG CTGCTTTTTA 1151 AATTCTTTAA TTACCTCCCC CTGCCCGAAT TGACGAGCAT CGTCAGCCTG 1201 CTGGTCATTT CTCTGTTTTT TGTAACTTCT GCCGATTCCG GGATTTATGT 1251 CCTGAACAAT ATTACCTCTC GGGACAAAGG CTTGAGCGCG CCACGGTGGC 1301 AGGCGGTTAT GTGGGGCGTG CTGATGTCTG CCGTTGCCGT TTTGCTGATG 1351 CGCTCGGGCG GACTCGGCAA CCTGCAGTCT ATGACCCTGA TTGTTTCCCT 1401 GCCGTTTGCC CTGCTGATGC TGATAATGTG TTTCAGCCTG TGGAAAGGCT 1451 TGAGTGCGGA TAAGAAATAT TTTGAGACCC GGGTTAACCC TACCAGTGTA 1501 TTTTGGACGG GCGGCAAGTG GAAAGAACGG CTGGTGCAGA TAATGAGCCA 1551 GACGCAGGAG CAGGATATTT TAAAATTCCT CAAACAGACT GCATCGCCCG 1601 CTATGCACGA GTTGCAACGG GAGCTTTCGG AAGAATACGG CTTGAGCGTC 1651 CGGGTCGATA AAATGTTTCA TCGGGACGAG CCCGCAATCG AGTTCGTCAT 1701 TCGGAAAGAG ACGATGCGCG ATTTTATGTA CGGGATTAAG TCTGTCGGGC 1751 AGGATGTATC CGACCAGTTG ATTAACGACG GCAAGCTGCC GCATATCCGG 1801 CATCAGACAA CTTACAAACC CTACGCTTAT TTTTTCGACG GGCGCGTCGG 1851 GTACGATGTG CAGTATATGA ACAAGGACGA GCTGATTGCC GACATTTTGA 1901 AAAACTACGA ACGTTATTTG ATGTTGTTGG ATGATGTCGG TCAGGAACTG 1951 ATGGCGCACG AGCAGGTGGA ATTGGCAGAG TAA This corresponds to the amino acid sequence <SEQ ID 676; ORF 163>: ml 63.pep 1 MVILTTLFFV CVLWLVLTV PDQVQMWLDR AKEVIFTEFS WFYVLTFSIF 51 LGFLLILSVS SLGNIRLGRD EDVPEFGFLS WLAMLFAAGM GVGLMFFGVA 101 EPLMHYFSDI TAGTPEHRQQ QALLHTVFHW GVHAWSVYGT IALALAYFGF 151 RYKLPLALRS CFYPLLKEKI SGRFGDAIDI MALLATFFGI ITTLGFGASQ 201 LGAGLQEMGW IAENSFSVQV_ LIIAAVMSLA VVSAISGVGK GVKVLSELNL 251 GLAFLLLFFV LAAGPTVYLL SAFGDNIGNY LGNLVRLSFK TYAYEREHKP 301 WFESWTVLYW AWWCSWAPFV GLFIARISKG RTIREFVFGV LLIPGLFGVL 351 WFTVFGNTAI WLNDGVAGGM LEKMTSSPET LLFKFFNYLP LPELTSIVSL 401 LVISLFFVTS ADSGIYVLNN ITSRDKGLSA PRWQAVMWGV LMSAVAVLLM. 451 RSGGLGNLQS MTLIVSLPFA LLMLIMCFSL WKGLSADKKY FETRVNPTSV 501 FWTGGKWKER LVQIMSQTQE QDILKFLKQT ASPAMHELQR ELSEEYGLSV 551 RVDKMFHRDE PAIEFVIRKE TMRDFMYGIK SVGQDVSDQL INDGKLPHIR 601 HQTTYKPYAY FFDGRVGYDV QYMNKDELIA DILKNYERYL MLLDDVGQEL 651 MAHEQVELAE * Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ml63 / gl63 98.6% identity in 660 aa overlap 10 20 30 40 50 60 ml63. pep MVILTTLFFVCVLWLVLTVPDQVQMWLDRAKEVIFTEFSWFYVLTFSIFLGFLLILSVS II II II II II II II I I I II I I I I II II I II I II II I I I I II II I I I II I II I I I I I II II gl63 MVILTTLFFVCVLWLVLTVPDQVQMWLDRAKEVIFTEFSWFYVLTFSIFLGFLLILSVS 10 20 30 40 50 60 70 80 90 100 110 120 ml 63. pep SLGΝIRLGRDEDVPEFGFLSWLAMLFAAGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQ : II II II II II II I I I I I I II I I I I I I I I I I I I I II II I II I II II II II I : I : II I I I I gl63 GLGΝIRLGRDEDVPEFGFLSWLAMLFAGMGVGLMFFGVAEPLMHYFSDITVGAPEHRQQ 70 80 90 100 110 120 130 140 150 160 170 180 ml 63. pep QALLHTVFHWGVHAWSVYGTIALALAYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDI II I I II II I II II I II II II II I I I II II I II II I II II II II II II I II I I II II I II I gl63 QALLHTVFHWGVHAWSVYGTIALALAYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDI 130 140 150 160 170 180 190 200 210 220 230 240 ml63. pep MALLATFFGIITTLGFGASQLGAGLQEMGWIAENSFSVQVLIIAAVMSLAWSAISGVGK II II II II I II II I I I I I I I II I I I II II I II II II : II II II II II I II I II I II I II I gl 63 MALLATFFGIITTLGFGASQLGAGLQEMGWIAENSFGVQVLIIAAVMSLAWSAISGVGK 190 200 210 220 230 240 250 260 270 280 290 300 ml 63. pep GVKVLSELNLGLAFLLLFFVLAAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKP II II II II II I I II II I I II II I I II II II I II II II II I I II II II : II I II I I I I I I gl 63 GVKVLSELNLGLAFLLLFFVLAADPTVYLLSAFGDNIGNYLGNLVRLSLKTYAYEREHKP 250 260 270 280 290 300 310 320 330 340 350 360 ml 63. pep WFESWTVLYWAWWCSWAPFVGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAI II II II II II I I II II I I II I I II II I I I II I I I I I II II I I II II I II I II I II II I II gl63 WFESWTVLYWAWWCSWAPFVGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAI 310 320 330 340 350 360 370 380 390 400 410 420 ml63.pep WLNDGVAGGMLEKMTΞSPETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNN II II II II II I I I I I II I II I I I I II II II I I I II I I I II II I I I II I II I II I II I II I gl 63 WLNDGVAGGMLEKMTSSPETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNN 370 380 390 400 410 420 430 440 450 460 470 480 ml63. pep ITSRDKGLSAPRWQAVMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSL II II II II II I I I I I II I I II II I II II II II I II I I I II II I II II I II I II I II II I I gl63 ITSRDKGLSAPRWQAVMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSL 430 440 450 460 470 480 490 500 510 520 530 540 ml63. pep WKGLSADKKYFETRVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKQTASPAMHELQR II I II I II I II II I II I I II II II II I I I I I I : I I I II II I I II II I I : I II I II II I II gl 63 WKGLSADKKYFETRVNPTSVFWTGGKWKERLVRIMSQTQEQDILKFLKHTASPAMHELQR 490 500 510 520 530 540 550 560 570 580 590 600 ml63. pep ELSEEYGLSVRVDKMFHRDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIR I I II I I I II II I I I I I I : II II II I II II I II II I I II I I II I II I II I I II II I II I II gl63 ELSEEYGLSVRVDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIR 550 560 570 580 590 600 610 620 630 640 650 660 ml63. pep HQTTYKPYAYFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE I II I II II I I I I II II I I I II II I I I I I I I II I I I II I I II II II I II I II I II I II I II gl63 HQTTYKPYAYFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE 610 620 630 640 650 660 ml 63. pep X I gl63 X The following partial DNA sequence was identified in N meningitidis <SEQ ID 677>: al63. seq 1 ATGGTTATTT TGACGACTTT GTTTTTTGTG TGTGTTTTGG TGGTATTGGT 51 TTTAACCGTG CCGGATCAGG TGCAGATGTG GCTCGATCGG GCAAAAGAAG 101 TCATTTTTAC CGAGTTCAGC TGGTTTTATG TTTTAACGTT TTCCATTTTT 151 CTGGGTTTCC TGCTGATACT CTCGGTCAGC AGTTTGGGAA ACATCAGGCT 201 CGGACGGGAT GAAGATGTGC CGGAATTCGG CTTCCTGTCG TGGCTGGCGA 251 TGCTGTTTGC GGCCGGGATG GGCGTGGGTC TGATGTTTTT CGGCGTGGCA 301 GAGCCGTTGA TGCATTATTT TTCGGACATT ACGGCCGGCA CGCCGGAACA 351 CAGGCAGCAG CAGGCATTGC TGCACACGGT GTTCCATTGG GGCGTTCACG 401 CTTGGTCGGT GTACGGTACG ATTGCATTGG CTTTGGCTTA TTTCGGTTTC 451 CGCTACAAGC TGCCGCTTGC CCTGCGTTCT TGTTTTTACC CCCTGTTGAA 501 AGAAAAAATT TCCGGAAGGT TCGGCGATGC CATTGATATT ATGGCGTTGC 551 TTGCTACTTT TTTCGGCATC ATCACCACAT TGGGGTTCGG GGCTTCGCAA 601 CTGGGCGCCG GATTGCAGGA AATAGGCTGG ATTGCCGAAA ACAGCTTCAG 651 CGTGCAGGTT TTGATTATCG CCGCCGTCAT GTCCCTCGCC GTCGTTTCGG 701 CAATATCCGG CGTGGGGAAG GGTGTGAAGG TGTTGAGCGA GTTGAACCTG 751 GGTCTTGCGT TTTTGCTGCT GTTTTTTGTT TTGGCGGCGG GTCCCACTGT 801 TTACCTGTTG TCGGCATTCG GCGACAACAT AGGGAACTAC CTCGGAAATC 851 TGGTGCGCCT CAGTTTTAAA ACTTATGCGT ACGAACGGGA ACACAAGCCG 901 TGGTTTGAAT CTTGGACGGT GCTTTATTGG GCGTGGTGGT GTTCTTGGGC 951 GCCGTTTGTG GGTTTGTTTA TCGCGCGCAT TTCAAAGGGG CGCACCATCC 1001 GCGAGTTTGT CTTCGGGGTT TTGCTCATCC CCGGCCTGTT CGGCGTTTTG 1051 TGGTTTACCG TCTTCGGCAA TACGGCGATT TGGCTGAATG ACGGGGTTGC 1101 GGGGGGAGTG CTCGAAAAGA TGACCTCCTC TCCGGAAACG CTGCTTTTTA 1151 AATTCTTTAA TTACCTCCCC CTGCCCGAAT TGACGAGCAT CGTCAGCCTG 1201 CTGGTCATTT CTCTGTTTTT TGTAACTTCT GCCGATTCCG GGATTTATGT 1251 CCTGAACAAT ATTACCTCTC GGGACAAAGG CTTGAGCGCG CCACGGTGGC 1301 AGGCGGTTAT GTGGGGCGTG CTGATGTCTG CCGTTGCCGT TTTGCTGATG 1351 CGCTCGGGCG GACTCGGCAA CCTGCAGTCT ATGACCCTGA TTGTTTCCCT 1401 GCCGTTTGCC CTGCTGATGC TGATAATGTG TTTCAGCCTG TGGAAAGGAT 1451 TGAGTGCGGA TAAGAAATAT TTTGAGACCC GGGTTAACCC TACCAGTGTA 1501 TTTTGGACGG GCGGCAAGTG GAAAGAACGG CTGGTGCAGA TAATGAGCCA 1551 GACGCAGGAG CAGGATATTT TAAAATTCCT CAAACATACC GCATCGCCCG 1601 CTATGCACGA GTTACAACGG GAGCTTTCGG AAGAATACGG CTTGAGCGTC 1651 CGGGTCGATA AGATGTTTCA TCAGGACGAG CCCGCAATCG AGTTCGTCAT 1701 TCGGAAAGAG ACGATGCGCG ATTTTATGTA CGGGATTAAG TCTGTCGGGC 1751 AGGATGTATC CGACCAGTTG ATTAACGACG GCAAGCTGCC GCATATCCGG 1801 CATCAGACAA CTTACAAACC CTACGCTTAT TTTTTCGACG GGCGCGTCGG 1851 GTACGATGTG CAGTATATGA ACAAGGACGA GCTGATTGCC GACATTTTGA 1901 AAAACTACGA ACGTTATTTG ATGTTGTTGG ATGATGTCGG TCAGGAACTG 1951 ATGGCGCACG AGCAGGTGGA ATTGGCAGAG TAA This corresponds to the amino acid sequence <SEQ ID 678; ORF 163.a>: al63.pep 1 MVILTTLFFV CVLWLVLTV PDQVQMWLDR AKEVIFTEFS WFYVLTFSIF 51 LGFLLILSVS SLGNIRLGRD EDVPEFGFLS WLAMLFAAGM GVGLMFFGVA 101 EPLMHYFSDI TAGTPEHRQQ QALLHTVFHW GVHAWSVYGT IALALAYFGF 151 RYKLPLALRS CFYPLLKEKI SGRFGDAIDI MALLATFFGI ITTLGFGASQ 201 LGAGLQEIGW IAENSFSVQV LIIAAVMSLA WSAISGVGK GVKVLSELNL 251 GLAFLLLFFV LAAGPTVYLL SAFGDNIGNY LGNLVRLSFK TYAYEREHKP 301 WFESWTVLYW AWWCSWAPFV GLFIARISKG RTIREFVFGV LLIPGLFGVL 351 WFTVFGNTAI WLNDGVAGGV LEKMTSSPET LLFKFFNYLP LPELTSIVSL 401 LVISLFFVTS ADSGIYVLNN ITSRDKGLSA PRWQAVMWGV LMSAVAVLLM 451 RSGGLGNLQS MTLIVSLPFA LLMLIMCFSL WKGLSADKKY FETRVNPTSV 501 FWTGGKWKER LVQIMSQTQE QDILKFLKHT ASPAMHELQR ELSEEYGLSV 551 RVDKMFHQDE PAIEFVIRKE TMRDFMYGIK SVGQDVSDQL INDGKLPHIR 601 HQTTYKPYAY FFDGRVGYDV QYMNKDELIA DILKNYERYL MLLDDVGQEL 651 MAHEQVELAE * ml63/al63 99.4% identity in 660 aa overlap 10 20 30 40 50 60 ml 63. pep MVILTTLFFVCVLWLVLTVPDQVQMWLDRAKEVIFTEFSWFYVLTFSIFLGFLLILSVS II I I I II I II II II I II II II II I I I I I II I II II II I II I I I II II I II I I II I I I II I al63 MVILTTLFFVCVLWLVLTVPDQVQMWLDRAKEVIFTEFSWFYVLTFSIFLGFLLILSVS 10 20 30 40 50 60 70 80 90 100 110 120 ml 63. pep SLGNIRLGRDEDVPEFGFLSWLAMLFAAGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQ I II I I I I I II II II II I II II II II II I II I I I II II II I II II I I II II I II II I II II al63 SLGNIRLGRDEDVPEFGFLSWLAMLFAAGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQ 70 80 90 100 110 120 130 140 150 160 170 180 ml63. pep QALLHTVFHWGVHAWSVYGTIALALAYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDI II I II I I II II II I I II I II II II II I II II II I II II I II II II II II II I II I II II I al63 QALLHTVFHWGVHAWSVYGTIALALAYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDI 130 140 150 160 170 180 190 200 210 220 230 240 ml63. pep MALLATFFGIITTLGFGASQLGAGLQEMGWIAENSFSVQVLIIAAVMΞLAWSAISGVGK II II II II I I I II I I II II II II II II : II II I I I II II I II I I I II I II I II II I II I I al63 MALLATFFGIITTLGFGASQLGAGLQEIGWIAENSFSVQVLIIAAVMSLAWSAISGVGK 190 200 210 220 230 240 250 260 270 280 290 300 ml 63. pep GVKVLSELNLGLAFLLLFFVLAAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKP I II I I I I II I II I I II I II II I II II II I II II I I I I II II II I I I II II I I I I II I I II al63 GVKVLSELNLGLAFLLLFFVLAAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKP 250 260 270 280 290 300 310 320 330 340 350 360 ml63. pep WFESWTVLYWAWWCSWAPFVGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAI I II II I I II II I I II II I II II I I II I II I I II I II II I II II II I I I I II I II I II I I I al63 WFESWTVLYWAWWCSWAPFVGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAI 310 320 330 340 350 360 370 380 390 400 410 420 ml63.pep WLNDGVAGGMLEKMTSSPETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNN I II I I I I II : II I I II I II I II I I II II I II I I I I II II II II I II II II I I I II I I II I al63 WLNDGVAGGVLEKMTSSPETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNN 370 380 390 400 410 420 430 440 450 460 470 480 ml 63. pep ITSRDKGLSAPRWQAVMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSL II I II II I I I I II II I I II II I I II I II I I II I I I II II I II I I II II II I II II I I I II al63 ITSRDKGLSAPRWQAVMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSL 430 440 450 460 470 480 490 500 510 520 530 540 ml63. pep WKGLSADKKYFETRVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKQTASPAMHELQR I II I I I I II II II I I II I I II I I II I II II II I II II II I II I I II II : I I I I I II I II I al63 WKGLSADKKYFETRVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQR 490 500 510 520 530 540 550 560 570 580 590 600 ml63. pep ELSEEYGLSVRVDKMFHRDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIR I II I I I I II I I I I I I II : I II II II I II I I I II I I II II I II I I II II II I II II II II I al63 ELSEEYGLSVRVDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIR 550 560 570 580 590 600 610 620 630 640 650 660 ml 63. pep HQTTYKPYAYFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE I II I I I I II II II I I I I II II I I I II II I II I I I I I II I II II I I I I I I II I II I II I I I al63 HQTTYKPYAYFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE 610 620 630 640 650 660 ml63.pep X I al63 X The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 679>: gl64. seq (partial) 1 ..ATGAACACAT TTTTGAAAAA CAGCGAATAC GCGTATATCC TGAACGACTG 51 CAAGGCGCGC TTCCTGTTCG CCTCGGCCGG CCTGTCAAAA GAATTGGCGG 101 GCCTGAAGGC GCAAACGCCC GTCGAAAAAA TCATTTGGAC GGACAAAAGC 151 CGGCCGGCCG GCGAAACGGC GGAAGGCGAT GCCTTTTTTG AAAACGTGCG 201 CCGCTTCCCC GAAAAACCCG ACTTGGGCCG CCAACCCCGG ATAAATGATT 251 TGGCACACAT CATCTACACC TCCGGCACGA CGGGGCATCC CAAAGGCGCG 301 CTAATCAGTT ACGCCAACCT GTTCGCCAAC CTGAACGGCA TCGAACGCAT 351 CTTtaaAATT TCCAAACGCG ACCGCTTTAT CGTTTTCctg ccgatgTTCC 401 ACAGCTTCAC GCTGACGGCT ATGGTGCTGC TGCCGATTTA TATGGCGTGT 451 TCGATTATTT TGGTCAAAtc cgttttCCCc ttttccaacG TTTTGAAACA 501 GGCCCTGCTC AAACGCGCAA CCGTGTTTTT GGGCGTACCC GCGATTTACA 551 CCGCGATGAG CAAGGCAAAA ATCCCTTGGT ATTTCAGATG GTTCAACCGC 601 ATCCGCCTGT TTATCAGCGG CGGCGCGCCT TTGGCGGAAC AAACCATCCT 651 CGATTTTAAA GCCAAGTTCC CCCGCGCCAA ATTGCTGGAA GGCTACGGAC 701 TGAGCGAAGC CTCGCCCGTC GTCGCCGTCA ATACGCCCGA ACGGCAAAAA 751 GCCCGCAGCG TCGGCATCCC CCTGCCCGGT TTGGAAGCCA AAGCCGTCGA 801 TGAAGAATTG GTCGAAGTGC CGCGCGGCGA AGTGGGCGAA CTGATCGTCA 851 GGGGCGGTTC GGTGATGCGG GGCTACCTCA ATATGCCTGC CGCCACCGAT 901 GAAACCATCG TCAACGGCTG GTTGAAAACG GGCGATTTCG TTACCATAGA 951 CGAGGACGGC TTTATCTTTA TCGTCGACCG CAAAAAAGAT TTGATTATTT 1001 CCAAAGGTCA AAACGTCTAT CCGCGCGAGA TCGAAGAAGA AATCCACAAA 1051 CTCGATGCCG TCGAAGCCGC CGCCGTCATC GGCGTGAAAG ACCGTTATGC 1101 CGACGAGGAA ATCGTCGCCT TCGTCCAATT GAAGGAAGGT ATGGATTTGG 1151 GCGAGGACGA aatccgccgc caccTGCGTA CCGTGCTGGC AAATTTCAAA 1201 ATCCCCAAAC AGATCCACTT TAAAGACGGG CTGCCGCGCA ACGCTACGGG 1251 CAAAGTATTG AAACGGGTGC TGAAGGAGCA GTTTGAAGGA AACAAATGA This corresponds to the amino acid sequence <SEQ ID 680; ORF 164.ng>: gl64.pep (partial) 1 ..MΝTFLKΝSEY AYILΝDCKAR FLFASAGLSK ELAGLKAQTP VEKIIWTDKS 51 RPAGETAEGD AFFEΝVRRFP EKPDLGRQPR IΝDLAHIIYT SGTTGHPKGA 101 LISYAΝLFAΝ LΝGIERIFKI SKRDRFIVFL PMFHSFTLTA MVLLPIYMAC 151 SIILVKSVFP FSΝVLKQALL KRATVFLGVP AIYTAMSKAK IPWYFRWFΝR 201 IRLFISGGAP LAEQTILDFK AKFPRAKLLE GYGLSEASPV VAVΝTPERQK 251 ARSVGIPLPG LEAKAVDEEL VEVPRGEVGE LIVRGGSVMR GYLΝMPAATD 301 ETIVΝGWLKT GDFVTIDEDG FIFIVDRKKD LIISKGQΝVY PREIEEEIHK 351 LDAVEAAAVI GVKDRYADEE IVAFVQLKEG MDLGEDEIRR HLRTVLAΝFK 401 IPKQIHFKDG LPRΝATGKVL KRVLKEQFEG ΝK* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 681>: ml6 . seq 1 ATGAACCGGA CTTATGCCAA TTTCTACGAA ATGCTCGCCG CCGCCTGCCG 51 CAAAAACGGA AACGGCACGG CAGTGTTCGA CGGCAAGGAA AAAACCGCCT 101 ACCGCGCGCT CAAGCAGGAG GCCGAAGCCG TCGCGGCGTA TCTGCAAAAT 151 ATCGGCGTGA AGTTCGGCGA CACGGTCGCG CTGGCGGTTT CCAATTCCAC 201 AGAATTTATT ACCGCCTATT TCGCCATCTC CGCCATCGGC GCGGTCGCCG 251 TACCGATGAA CACATTTTTG AAAAACAGCG AATACGCGTA TATCCTGAAC 301 GACTGCAAGG CGCGCTTCCT GTTCGCCTCG GCCGGCCTGT CAAAAGAATT 351 GGCGGGCTTG AAGGCGCAAA CGCCCGTCGA AAAAATCATT TGGACGGACA 401 AAAGCCGTCC GACCGGCGAA ACGGCGGAAG GCGATGCCTT TTTTGAAGAC 451 GTGCGCCGCT TCCCCGAAAA ACCCGACTTG GGCCGCCAAC CCCGGATAAA 501 TGATTTGGCA CACATCATCT ACACCTCCGG CACGACGGGG CATCCCAAAG 551 GCGCGCTAAT CAGTTACGCC AACCTGTTCG CCAACCTGAA CGGCATCGAA 601 CGCATCTTTA AAATTTCCAA GCGCGACCGC TTTATCGTTT TCCTGCCGAT 651 GTTCCACAGC TTCACGCTGA CGGCTATGGT GCTGCTGCCG ATTTATATGG 701 CGTGTTCGAT TATTTTGGTC AAATCCGTTT TTCCGTTTTC CAACGTTTTG 751 AAACAGACAC TGCTCAAACG CGCGACCGTG TTTTTGGGCG TACCCGCGAT 801 TTACACCGCG ATGAGCAAGG CGAAAATCCC TTGGTATTTC AGATGGTTCA 851 ACCGCATTCG CCTGTTTATC AGCGGCGGCG CGCCTTTGGC GGAACAAACC 901 ATCCTCGATT TCAAAGCCAA GTTCCCCCGC GCCAAATTGC TGGAAGGCTA 951 CGGACTGAGC GAAGCCTCTC CCGTCGTCGC CGTCAATACG CCCGAGAGGC 1001 AAAAAGCCCG CAGCGTCGGC ATCCCCCTGC CCGGTTTGGA AGCCAAAGCC 1051 GTCGATGAAG AATTGGTCGA AGTGCCGCGC GGCGAAGTGG GCGAACTGAT 1101 CGTCAGGGGC GGTTCGGTGA TGCGGGGCTA CCTCAATATG CCTGCCGCCA 1151 CCGATGAAAC CATCGTCAAC GGCTGGTTGA AAACGGGCGA TTTCGTTACC 1201 ATAGACGAAG ACGGCTTTAT CTTTATCGTC GACCGCAAAA AAGATTTGAT 1251 TATTTCCAAA GGTCAAAATG TCTATCCGCG CGAGATTGAA GAAGAAATCT 1301 ACAAACTCGA TGCCGTCGAA GCCGCCGCCG TCATCGGCGT GAAAGACCGT 1351 TATGCCGACG AGGAAATCGT CGCCTTCGTC CAATTGAAGG AAGGTATGGA 1401 TTTGGGCGAG AACGAAATCC GCCGCCACCT GCGTACCGTG CTGGCAAATT 1451 TCAAAATCCC CAAACAAATC CACTTTAAAG ACGGGCTGCC GCGCAACGCT 1501 ACGGGCAAGG TATTGAAACG GGTGTTGAAG GAGCAGTTTG ACGGAAACAA 1551 ATGA This corresponds to the amino acid sequence <SEQ ID 682; ORF 164>: ml64.pep 1 MNRTYANFYE MLAAACRKNG NGTAVFDGKE KTAYRALKQE AEAVAAYLQN 51 IGVKFGDTVA LAVSNSTEFI TAYFAISAIG AVAVPMNTFL KNSEYAYILN 101 DCKARFLFAS AGLSKELAGL KAQTPVEKII WTDKSRPTGE TAEGDAFFED 151 VRRFPEKPDL GRQPRINDLA HIIYTSGTTG HPKGALISYA NLFANLNGIE 201 RIFKISKRDR FIVFLPMFHS FTLTAMVLLP IYMACSIILV KSVFPFSNVL 251 KQTLLKRATV FLGVPAIYTA MSKAKIPWYF RWFNRIRLFI SGGAPLAEQT 301 ILDFKAKFPR AKLLEGYGLS EASP AVNT PERQKARSVG IPLPGLEAKA 351 VDEELVEVPR GEVGELIVRG GSVMRGYLNM PAATDETIVN GWLKTGDFVT 401 IDEDGFIFIV DRKKDLIISK GQNVYPREIE EEIYKLDAVE AAAVIGVKDR 451 YADEEIVAFV QLKEGMDLGE NEIRRHLRTV LANFKIPKQI HFKDGLPRNA 501 TGKVLKRVLK EQFDGNK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ml64 / gl64 98.6% identity in 432 aa overlap 60 70 80 90 100 110 ml64. pep GDTVALAVSΝSTEFITAYFAISAIGAVAVPMΝTFLKΝSEYAYILΝDCKARFLFASAGLSK II I I I I II II I I I II II I II II II I I I I I I gl64 MΝTFLKΝSEYAYILΝDCKARFLFASAGLSK 10 20 30 120 130 140 150 160 170 ml64. pep ELAGLKAQTPVEKIIWTDKSRPTGETAEGDAFFEDVRRFPEKPDLGRQPRIΝDLAHIIYT II I II II II I II I I I I I II I I I: I I I I I I II I I I : I I I I II I I II I I II I I I I I II II I I gl64 ELAGLKAQTPVEKIIWTDKSRPAGETAEGDAFFEΝVRRFPEKPDLGRQPRIΝDLAHIIYT 40 50 60 70 80 90 180 190 200 210 220 230 ml64. pep SGTTGHPKGALISYAΝLFAΝLΝGIERIFKISKRDRFIVFLPMFHSFTLTAMVLLPIYMAC I II II II I II II II I I I I I I I II I I I I II I I II I II I I I II I I II II I II II I I I I I II I gl64 SGTTGHPKGALISYAΝLFAΝLΝGIERIFKISKRDRFIVFLPMFHSFTLTAMVLLPIYMAC 100 110 120 130 140 150 240 250 260 270 280 290 ml64. pep SIILVKSVFPFSΝVLKQTLLKRATVFLGVPAIYTAMSKAKIPWYFRWFΝRIRLFISGGAP I II I I I I I I I I II I I I I : II I I II I I I I I I I I I I I I I II I I I II I I II I I I II II I II II gl64 SIILVKSVFPFSΝVLKQALLKRATVFLGVPAIYTAMSKAKIPWYFRWFΝRIRLFISGGAP 160 170 180 190 200 210 300 310 320 330 340 350 ml64.pep LAEQTILDFKAKFPRAKLLEGYGLSEASPWAVΝTPERQKARSVGIPLPGLEAKAVDEEL II I I I II II I II I II I II I I I II I I II II I I II I II II II I I I I I II I I I II II I II II I g164 LAEQTILDFKAKFPRAKLLEGYGLSEASPWAVΝTPERQK RSVGIPLPGLEAKAVDEEL 220 230 240 250 260 270 360 370 380 390 400 410 ml64.pep VEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGWLKTGDFVTIDEDGFIFIVDRKKD I I I II I I II I II II II II I II II II I II II II II I I I II II II II I II II II II II I II I gl64 VEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGWLKTGDFVTIDEDGFIFIVDRKKD 280 290 300 310 320 330 420 430 440 450 460 470 ml 64. pep LIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDRYADEEIVAFVQLKEGMDLGENEIRR I I II I I I II I II II I I II : I II I II I II I I I I II I I II I II II I I I II II II II I : I I I I gl64 LIISKGQNVYPREIEEEIHKLDAVEAAAVIGVKDRYADEEIVAFVQLKEGMDLGEDEIRR 340 350 360 370 380 390 480 490 500 510 ml64. pep HLRTVLANFKIPKQIHFKDGLPRNATGKVLKRVLKEQFDGNKX II II I I I I I I II I I II I I I II II II I II II II II I I II : II II gl64 HLRTVLANFKIPKQIHFKDGLPRNATGKVLKRVLKEQFEGNKX 400 410 420 430 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 683>: al64. seq 1 ATGAACCGGA CTTATGCCAA TTTCTACGAA ATGCTGACCG CCGCCTGCCG 51 CAAAAACGGC AACGGCACGG CAGTGTTCGA CGGCAAGGAA AAAACCGCCT 101 ACCGCGCGCT CAAGCAGGAA GCCGAAGCCG TTGCGGCGTA TCTGCAAAAT 151 ATCGGCGTGA AGTTCGGCGA CACGGTCGCG CTGGCGGTTT CCAATTCCAC 201 GGAATTTATT ACCGCCTATT TCGCCGTATC CGCCATCGGC GCGGTTGCCG 251 TACCGATGAA CACATTTTTG AAAAACAGCG AATACGCGTA TATCCTGAAC 301 GACTGCAAGG CGCGCTTCCT GTTCGCCTCG GCCGGCCTGT CAAAAGAATT 351 GGCGGGCTTG AAGGCGCAAA CGCCCGTCGA AAAAATCATT TGGACGGGCC 401 AAAGCCGTCC GGACGGCGAA ATGGCGGAAG GCGATGCCTT TTTTGAAGAC 451 GTGCGCCGCT TCCCCGAAAA ACCCGACTTG GGCCGCCAAC CCCGGATAAA 501 TGATTTGGCA CACATCATCT ACACCTCCGG CACGACGGGG CATCCCAAAG 551 GTGCGCTAAT CAGCTACGCC AACCTGTTCG CCAACCTGAA CGGCATCGAA 601 CGCATCTTTA AAATCTCCAA GCGCGACCGC TTTATCGTTT TCCTGCCGAT 651 GTTCCACAGC TTCACGCTGA CGGCTATGGT GCTGCTGCCG ATTTATATGG 701 CGTGTTCGAT TATTTTGGTC AAATCCGTTT TCCCCTTTTC CAACGTTTTG 751 AAACAGGCAC TGCTCAAACG CGCGACCGTG TTTTTGGGCG TGCCCGCGAT 801 TTACACCGCG ATGAGCAAGA CGAAAATCCC TTGGTATTTC AGATGGTTCA 851 ACCGCATCCG CCTGTTTATC AGCGGCGGAG CACCTTTGGC GGAACAAACC 901 ATCCTCGATT TCAAAGCCAA GTTCCCCCGC GCCAAATTGC TGGAAGGCTA 951 CGGACTGAGC GAAGCCTCGC CCGTCGTCGC CGTCAATACG CCCGAGAGGC 1001 AAAAAGCCCG CAGCGTCGGC ATCCCCCTGC CCGGTTTGGA AGTCAAAGCC 1051 GTCGATGAAG AATTGGTCGA AGTGCCGCGC GGCGAAGTGG GCGAACTGAT 1101 CGTCAGGGGC GGTTCGGTGA TGCGGGGCTA CCTCAATATG CCTGCCGCCA 1151 CCGATGAAAC CATCGTCAAC GGCTGGTTGA AAACGGGCGA TTTCGTTACC 1201 ATAGACGAAG ACGGCTTTAT CTTTATCGTC GACCGCAAAA AAGATTTGAT 1251 TATTTCCAAA GGTCAAAATG TCTATCCGCG CGAAATCGAA GAAGAAATCT 1301 ACAAACTCGA TGCCGTCGAA GCCGCCGCCG TCATCGGCGT GAAAGACCGT 1351 TATGCCGACG AGGAAATCGT CGCCTTCGTC CAATTGAAGG AAGGTATGGA 1401 TTTGGGCGAG AACGAAATCC GCCGCCACCT GCGTACCGTG CTGGCAAATT 1451 TCAAAATCCC CAAACAAATC CACTTTAAAG ACGGGCTGCC GCGCAACGCT 1501 ACGGGCAAGG TATTGAAACG GGTGTTGAAG GAGCAGTTTG ACGGAAACAA 1551 ATGA This corresponds to the amino acid sequence <SEQ ID 684; ORF 164.a>: al64.pep 1 MΝRTYAΝFYE MLTAACRKΝG NGTAVFDGKE KTAYRALKQE AEAVAAYLQN 51 IGVKFGDTVA LAVSNSTEFI TAYFAVSAIG AVAVPMNTFL KNSEYAYILN 101 DCKARFLFAS AGLSKELAGL KAQTPVEKII WTGQSRPDGE MAEGDAFFED 151 VRRFPEKPDL GRQPRINDLA HIIYTSGTTG HPKGALISYA NLFANLNGIE 201 RIFKISKRDR FIVFLPMFHS FTLTAMVLLP IYMACSIILV KSVFPFSNVL 251 KQALLKRATV FLGVPAIYTA MSKTKIPWYF RWFNRIRLFI SGGAPLAEQT 301 ILDFKAKFPR AKLLEGYGLS EASPWAVNT PERQKARSVG IPLPGLEVKA 351 VDEELVEVPR GEVGELIVRG GSVMRGYLNM PAATDETIVN GWLKTGDFVT 401 IDEDGFIFIV DRKKDLIISK GQNVYPREIE EEIYKLDAVE AAAVIGVKDR 451 YADEEIVAFV QLKEGMDLGE NEIRRHLRTV LANFKIPKQI HFKDGLPRNA 501 TGKVLKRVLK EQFDGNK* ml64/al64 98.3% identity in 517 aa overlap 10 20 30 40 50 60 ml64.pep MNRTYANFYEMLAAACRKNGNGTAVFDGKEKTAYRALKQEAEAVAAYLQNIGVKFGDTVA I I II II II II II : I II II I II II II I II II II II II I I II I I I I II II II II II II I II I al64 MNRTYANFYEMLTAACRKNGNGTAVFDGKEKTAYRALKQEAEAVAAYLQNIGVKFGDTVA 10 20 30 40 50 60 70 80 90 100 110 120 ml 64. pep LAVSNSTEFITAYFAISAIGAVAVPMNTFLKNSEYAYILNDCKARFLFASAGLSKELAGL I I II II II I II I II I : II I II II I II II II I II II I I I II I I I I II II II I I II II I I II al64 LAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDCKARFLFASAGLSKELAGL 70 80 90 100 110 120 130 140 150 160 170 180 ml 64. pep KAQTPVEKIIWTDKSRPTGETAEGDAFFEDVRRFPEKPDLGRQPRINDLAHIIYTSGTTG II II I I I II II I M II II I I II II II I II II II I I I I I I I II II II II I I II I I I al64 KAQTPVEKIIWTGQSRPDGEMAEGDAFFEDVRRFPEKPDLGRQPRINDLAHIIYTSGTTG 130 140 150 160 170 180 190 200 210 220 230 240 ml64.pep HPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMFHSFTLTAMVLLPIYMACSIILV II I I II I II II II II II II I I I II I II II II I II II II I I II I I I II II I I I I II II I I I al64 HPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMFHSFTLTAMVLLPIYMACSIILV 190 200 210 220 230 240 250 260 270 280 290 300 ml64. pep KSVFPFSNVLKQTLLKRATVFLGVPAIYTAMSKAKIPWYFRWFNRIRLFISGGAPLAEQT II I II II II I II: II II I II II II I II I II I II: I II II II I I I I II I II II II II II I I al64 KSVFPFSNVLKQALLKRATVFLGVPAIYTAMSKTKIPWYFRWFNRIRLFISGGAPLAEQT 250 260 270 280 290 300 310 320 330 340 350 360 ml64. pep ILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQKARSVGIPLPGLEAKAVDEELVEVPR I II II II I II I II II II I II I II I II II II I II II II II II I II I I I : II II II II I I II al64 ILDFKAKFPRAKLLEGYGLSEASPWAVNTPERQKARSVGIPLPGLEVKAVDEELVEVPR 310 320 330 340 350 360 370 380 390 400 410 420 ml64.pep GEVGELIVRGGSVMRGYLNMPAATDETIVNGWLKTGDFVTIDEDGFIFIVDRKKDLIISK II I II II II I II I II II I II II II II II II II I II II II II II II II I II II II II I I II al64 GEVGELIVRGGSVMRGYLNMPAATDETIVNGWLKTGDFVTIDEDGFIFIVDRKKDLIISK 370 380 390 400 410 420 430 440 450 460 470 480 ml64. pep GQNVYPREIEEEIYKLDAVEAAAVIGVKDRYADEEIVAFVQLKEGMDLGENEIRRHLRTV I I I I I I II I II I II II II II II II I II I I I I II II II II II II I II I I II II II II I I II al64 GQNVYPREIEEEIYKLDAVEAAAVIGVKDRYADEEIVAFVQLKEGMDLGENEIRRHLRTV 430 440 450 460 470 480 490 500 510 ml64. pep LANFKIPKQIHFKDGLPRNATGKVLKRVLKEQFDGNKX I I II II II I II I II II II II II II II I I I I I II II II I al64 LANFKIPKQIHFKDGLPRNATGKVLKRVLKEQFDGNKX 490 500 510 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 685>: gl65. seq 1 ATGGCTGAAG CGACAGACGT TGTCTTGGTG GGCGGCGGCA TTATGAGCGC 51 GACTTTGGGC GTTTTGCTCA AAGAACTCGA ACCGTCTTGG GAAATCACCC 101 TGATTGAACG CTTGGAagat gTGGCGTTGG AATCGTCAAA cGCGTGGAAC 151 AACGcCGgca CGGGGCATTC CGcGCTGTGc GAATTGAACT AtgcgccGCT 201 GGGtgcggac ggcgtcatCA ATCCGGCGCg cgCCCTGAAT ATTGCCGAAC 251 AGTTTCATGT CAGCCGCCAG TTTTGGGcga cgctgGTCGC GGAAGGCAAG 301 TTGGAagaCA ATTCCTTCAT CAATGCcgtg ccgcatatGT Ctttggtgat 351 gAacgaagac cactgCCgtt acCTGCAAAA ACGCTATGAT GTGTTTAAAA 401 CGCAGAAACT TTTTGAAAAT ATGGAATTTT CCACCGATCG GAACAAAATT 451 TCCGATTGGG CtccgCTGAT TATGCGCGGC CGGgacgaaA ACCAACCCGT 501 CGCCGCCAAC TATTCCGCCG Aaggcacgga tgtcgATTTC GGACGGCTGA 551 CGCGCCAGAT GGTGAAATAT TTGCAGGGCA AGGGCGTAAA AACCGAGTTC 601 AACCGCCACG TCGAAGACAT CAAACGCGAA TCCGACGGCG CGTGGGTGCT 651 CAAAACCGCC GATACCCGCA ACCCAGACTG GCAGCTCACC CTCCGCACCC 701 GCTTCCTCTT CCTCGGCGCG GGCGGCGGCG CACTGACCCT GCTGCAAAAA 751 TCCGGCATCC CCGAAGGCAA AGGCTACGGC GGCTTACCCG TGTCCGGCCT 801 GTTCTTCCGC AACAGCAACC CCGAAACCGC CGAACAACAC AACGCCAAAG 851 TGTACGGGCA GGCTTCCGTC GGCGCGCCGC CGATGTCCGT CCCGCACCTC 901 GACACACGCA ACGTAGACGG CAAACGACAC CTTATGTTCG GTCCTTACGC 951 AGGTTTCCGT TCCAACTTCC TCAAGCAAGG CTCGTTTATG GATTTGCCGC 1001 TGTCCATCCA TATGGACAAC CTCTATCCTA TGCTGCGCGC CGGCTGGGCG 1051 AATATGCCGC TGACCAAATA CcTGCTGGgC gAaTTGCgtt aa This corresponds to the amino acid sequence <SEQ ID 686; ORF 165.ng>: gl65.pep 1 MAEATDWLV GGGIMSATLG VLLKELEPSW EITLIERLED VALESSNAWN 51 NAGTGHSALC ELNYAPLGAD GVINPARALN IAEQFHVSRQ FWATLVAEGK 101 LEDNSFINAV PHMSLVMNED HCRYLQKRYD VFKTQKLFEN MEFSTDRNKI 151 SDWAPLIMRG RDENQPVAAN YSAEGTDVDF GRLTRQMVKY LQGKGVKTEF 201 NRHVEDIKRE SDGAWVLKTA DTRNPDWQLT LRTRFLFLGA GGGALTLLQK 251 SGIPEGKGYG GLPVSGLFFR NSNPETAEQH NAKVYGQASV GAPPMSVPHL 301 DTRNVDGKRH LMFGPYAGFR SNFLKQGSFM DLPLSIHMDN LYPMLRAGWA 351 NMPLTKYLLG ELR* The following partial DNA sequence was identified in N meningitidis <SEQ ID 687>: ml65. seq (partial) 1 ATGGCTGAAG CGACAGACGT TGTCTTGGTG GGCGGCGGCA TTATGAGCGC 51 GACTTTGGGC GTTTTGCTCA AAGAACTCGA ACCGTCTTGG GAAATCACCC 101 TGATTGAACG CTTGGAAGAT GTGGCGTTGG AATCGTCAAA CGCGTGGAAC 151 AACGCCGGCA CGGGGCATTC CGCGCTGTGC GAATTGAACT ATGCGCCGTT 201 GGGTGCAAAT GGGATTATCG ATCCGGCGCG CGCCCTCAAT ATTGCCGAAC 251 AGTTTCATGT CAGCCGCCAG TTTTGGGCGA CGCTGGTCGC GGAAGGCAAG 301 TTGGAAGACA ATTCCTTCAT CAATGCCGTG CCGCATATGT CTTTGGTGAT 351 GAATGAAGAC CATTGTTCTT ATCTTCAAAA ACGTTATGAC GCGTTTAAAA 401 CCCAAAAACT TTTTGAAAAT ATGGAATTTT CCACCGATCG GAACAAAATT 451 TCCGATTGGG CTCCGCTGAT GATGCGCGGC CGGGACGAAA ACCAACCCGT 501 CGCCGCCAAC TACTCCGCCG AAGgTACGGA TGTCGATTTC GGACGGCTGA 551 CGCGCCAAAT GGTGAAATAT TTGCAGGGCA AGGGCGTAAA AACCGAGTTC 601 AACCGCCACG TCGAAGACAT CAAACGCGAA TCCGACGGCG CGTGGGTGCT 651 CAAAACCGCC GATACCCGCA ACCCCGACGG GCAGCTCACC CTCCGTACCC 701 GCTTCCTCTT CCTCGGCGCG GGCGGCGGCG CGCTGACCCT GCTGCAAAAA 751 TCCGGCATCC CCGAAGGCAA AGGCTACGGC GGCTTCCCCG TGTCCGGCCT 801 GTTCTTCCGC AACAGCAACC CCGAAACCGC CGAACAACAC AACGCCAAAG 851 TGTACGGGCA GGCTTCCGTC GGCGCGCCGC CGATGTCCGT CCCGCACCTC 901 GACACACGCA ACGTGGACGG CAAACGCCAC CTTATGTTCG GCCCTTACGC 951 AGGCTTCCGT TCCAACTTCC TCAAGCAAGG CTCGCTTATG GATTTGCCGC 1001 TGTCCATCCA TATGGACAAC CTCTATCCTA TGCTGTGCGC CGGCTGGGCG 1051 AATATGCCGC TGACCAAA... This corresponds to the amino acid sequence <SEQ ID 688; ORF 165>: ml65.pep (partial) 1 MAEATDWLV GGGIMSATLG VLLKELEPSW EITLIERLED VALESSΝAWΝ 51 NAGTGHSALC ELNYAPLGAN GIIDPARALN IAEQFHVSRQ FWATLVAEGK 101 LEDNSFINAV PHMSLVMNED HCSYLQKRYD AFKTQKLFEN MEFSTDRNKI 151 SDWAPLMMRG RDENQPVAAN YSAEGTDVDF GRLTRQMVKY LQGKGVKTEF 201 NRHVEDIKRE SDGAWVLKTA DTRNPDGQLT LRTRFLFLGA GGGALTLLQK 251 SGIPEGKGYG GFPVSGLFFR NSNPETAEQH NAKVYGQASV GAPPMSVPHL 301 DTRNVDGKRH LMFGPYAGFR SNFLKQGSLM DLPLSIHMDN LYPMLCAGWA 351 NMPLTK... Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ml65 / gl65 97.2% identity in 356 aa overlap 10 20 30 40 50 60 ml65. pep MAEATDWLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALESSΝAWΝΝAGTGHSALC I II I I I I I I II I II II I I I II II I II I I I II I II I I I I I I II I I II II I I II II II I I II gl65 MAEATDWLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALESSΝAWΝΝAGTGHSALC 10 20 30 40 50 60 70 80 90 100 110 120 ml65. pep ELΝYAPLGAΝGIIDPARALΝIAEQFHVSRQFWATLVAEGKLEDΝSFIΝAVPHMSLVMΝED I I I II II II : I : I: I II I II II II I II I I I I I I I II II I II II II II II I I I I II I I II I gl65 ELΝYAPLGADGVIΝPARALΝIAEQFHVSRQFWATLVAEGKLEDΝSFIΝAVPHMSLVMΝED 70 80 90 100 110 120 130 140 150 160 170 180 ml65. pep HCSYLQKRYDAFKTQKLFEΝMEFSTDRΝKISDWAPLMMRGRDEΝQPVAAΝYSAEGTDVDF II II I I II I: I II I II I I II I I I II I I II II II I I: II I II II II II I I II II I II I I I gl65 HCRYLQKRYDVFKTQKLFEΝMEFSTDRΝKISDWAPLIMRGRDEΝQPVAAΝYSAEGTDVDF 130 140 150 160 170 180 190 200 210 220 230 240 ml65. pep GRLTRQMVKYLQGKGVKTEFΝRHVEDIKRESDGAWVLKTADTRΝPDGQLTLRTRFLFLGA II II II II II II I I II I II II II I II I I I II I I I II II I I II I I I I I I II I II II I I II gl65 GRLTRQMVKYLQGKGVKTEFΝRHVEDIKRESDGAWVLKTADTRΝPDWQLTLRTRFLFLGA 190 200 210 220 230 240 250 260 270 280 290 300 ml65. pep GGGALTLLQKSGIPEGKGYGGFPVSGLFFRΝSΝPETAEQHΝAKVYGQASVGAPPMSVPHL I I II II II II II I I II I I I II: I I II I I II I I I I I II I II II I I II II I I II II I II I I I gl65 GGGALTLLQKSGIPEGKGYGGLPVSGLFFRΝSΝPETAEQHΝAKVYGQASVGAPPMSVPHL 250 260 270 280 290 300 310 320 330 340 350 ml 65. pep DTRΝVDGKRHLMFGPYAGFRSΝFLKQGSLMDLPLSIHMDΝLYPMLCAGWAΝMPLTK I I I II II II I I I I II I II II I I I I I I I I : I I I I I I 1 II I I II I I I II I I II II I I gl65 DTRΝVDGKRHLMFGPYAGFRSΝFLKQGSFMDLPLSIHMDΝLYPMLRAGWAΝMPLTKYLLG 310 320 330 340 350 360 gl65 ELRX The following partial DΝA sequence was identified in N meningitidis <SEQ ID 689>: al65. seq 1 ATGGCTGAAG CGACAGACGT TGTCTTGGTG GGCGGCGGCA TTATGAGCGC 51 GACTTTGGGC GTTTTGCTCA AAGAACTCGA ACCGTCTTGG GAAATCACCC 101 TGATTGAACG CTTGGAAGAT GTGGCGTTGG AATCGTCAAA CGCGTGGAAC 151 AACGCCGGCA CGGGGCATTC CGCGCTGTGC GAATTGAACT ATGCGCCGTT 201 GGGTGCAAAT GGGATTATCG ATCCGGCGCG CGCCCTCAAT ATTGCCGAAC 251 AGTTTCATGT CAGCCGCCAG TTTTGGGCGA CGTTGGTCGC GGAAGGCAAG 301 TTGGAAGACA ATTCCTTCAT CAATGCCGTG CCGCATATGT CTTTGGTGAT 351 GAATGAAGAC CATTGTTCTT ATCTTCAAAA ACGTTATGAC GCGTTTAAAA 401 CCCAAAAACT TTTTGAAAAT ATGGAATTTT CCACCGATCG GAACAAAATT 451 TCCGATTGGG CTCCGCTGAT GATGCGCGGC CGGGACGAAA ACCAACCCGT 501 CGCCGCCAAC TACTCCGCCG AAGGCACGGA TGTCGATTTC GGACGGCTGA 551 CGCGCCAAAT GGTGAAATAT TTGCAGGGCA AGGGCGTAAA AACCGAGTTC 601 AACCGCCACG TCGAAGACAT CAAACGCGAA TCCGACGGCG CGTGGGTGCT 651 CAAAACCGCC GATACCCGCA ACCCCGACGG GCAGCTCACC CTCCGTACCC 701 GCTTCCTCTT CCTCGGCGCG GGCGGCGGCG CGCTGACCCT GCTGCAAAAA 751 TCCGGCATCC CCGAAGGCAA AGGCTACGGT GGCTTTCCCG TGTCCGGCCT 801 GTTCTTCCGC AACAGCAACC CCGAAACCGC CGAACAACAC AACGCCAAAG 851 TGTACGGGCA GGCTTCCGTC GGCGCGCCGC CGATGTCCGT CCCGCACCTC 901 GACACACGCA ACGTGGACGG CAAACGCCAC CTTATGTTCG GCCCTTACGC 951 AGGCTTCCGT TCCAACTTCC TCAAGCAAGG CTCACTTATG GATTTGCCGC 1001 TGTCCATCCA TATGGACAAC CTCTATCCTA TGCTGCGCGC CGGCTGGGCG 1051 AATATGCCGC TGACCAAATA CCTGCTGGGC GAATTGCGTA AAACCAAAGA 1101 AGAACGCTTC GCCTCCCTGC TGGAATACTA CCCCGAGGCA AACCCCGACG 1151 ACTGGGAACT CATCACCGCA GGGCAACGCG TTCAAATCAT TAAAAAAGAC 1201 TCCGAAAAAG GCGGCGTGTT GCAGTTTGGT ACGGAGATTG TCGCACACGC 1251 CGACGGCTCG CTCGCCGCAT TGCTGGGCGC GTCGCCGGGC GCATCGACCG 1301 CCGTGCCGCT GATGATCCGG CTGATGCACC AATGCTTCCC CGAACGCACC 1351 CCGTCTTGGG AAGGCCGTCT GAAAGAGCTG GTACCGGGTT ACGGCATCAA 1401 GTTGAACGAA AACCCCGAAA GGGCGGATGA AATTATCGCC TATACCGCGA 1451 AAGTGTTGGA TATTTAA This corresponds to the amino acid sequence <SEQ ID 690; ORF 165.a>: al65.pep 1 MAEATDWLV GGGIMSATLG VLLKELEPSW EITLIERLED VALESSNAWN 51 NAGTGHSALC ELNYAPLGAN GIIDPARALN IAEQFHVSRQ FWATLVAEGK 101 LEDNSFINAV PHMSLVMNED HCSYLQKRYD AFKTQKLFEN MEFSTDRNKI 151 SDWAPLMMRG RDENQPVAAN YSAEGTDVDF GRLTRQMVKY LQGKGVKTEF 201 NRHVEDIKRE SDGAWVLKTA DTRNPDGQLT LRTRFLFLGA GGGALTLLQK 251 SGIPEGKGYG GFPVSGLFFR NSNPETAEQH NAKVYGQASV GAPPMSVPHL 301 DTRNVDGKRH LMFGPYAGFR SNFLKQGSLM DLPLSIHMDN LYPMLRAGWA 351 NMPLTKYLLG ELRKTKEERF ASLLEYYPEA NPDDWELITA GQRVQIIKKD 401 SEKGGVLQFG TEIVAHADGS LAALLGASPG ASTAVPLMIR LMHQCFPERT 451 PSWEGRLKEL VPGYGIKLNE NPERADEIIA YTAKVLDI* ml65/al65 99.7% identity in 356 aa overlap 10 20 30 40 50 60 ml65. pep MAEATDWLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALESSNAWNNAGTGHSALC I I I I I I I II I I II II I II I I II II I I I I II II I I I II I II I II II II II I II I II I II I I al65 MAEATDWLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALESSNAWNNAGTGHSALC 10 20 30 40 50 60 70 80 90 100 110 120 ml65.pep ELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLEDNSFINAVPHMSLVMNED II II II II I I I II I I II I I I I II II I II I I II I II II II II II II II II I I I I II II I II al65 ELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLEDNSFINAVPHMSLVMNED 70 80 90 100 110 120 130 140 150 160 170 180 ml 65. pep HCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRGRDENQPVAANYSAEGTDVDF I I II II II II II I I I II I I II II I I I II I I II I II II II I I II II II II I I I I I I I II I I al 65 HCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRGRDENQPVAANYSAEGTDVDF 130 140 150 160 170 180 190 200 210 220 230 240 ml 65. pep GRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLKTADTRNPDGQLTLRTRFLFLGA I I II I I I II I II I I II I I I II II I II II I I I II II II II II II II II I II I I I I I I I II I al65 GRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLKTADTRNPDGQLTLRTRFLFLGA 190 200 210 220 230 240 250 260 270 280 290 300 ml65. pep GGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPETAEQHNAKVYGQASVGAPPMSVPHL II M II II I I II I I I II I I I II II I I I II II I II I I I I II I I II II II II II II II I I I I al65 GGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPETAEQHNAKVYGQASVGAPPMSVPHL 250 260 270 280 290 300 310 320 330 340 350 ml 65. pep DTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPLSIHMDNLYPMLCAGWANMPLTK I II I I I II II II II II II II II II II II II I I I II II II II II I I II II I II II I al65 DTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPLSIHMDNLYPMLRAGWANMPLTKYLLG 310 320 330 340 350 360 al65 ELRKTKEERFASLLEYYPEANPDDWELITAGQRVQIIKKDSEKGGVLQFGTEIVAHADGS 370 380 390 400 410 420 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 691 > • gl65-l.seq 1 ATGGCTGAAG CGACAGACGT TGTCTTGGTG GGCGGCGGCA TTATGAGCGC 51 GACTTTGGGC GTTTTGCTCA AAGAACTCGA ACCGTCTTGG GAAATCACCC 101 TGATTGAACG CTTGGAagat gTGGCGTTGG AATCGTCAAA cGCGTGGAAC 151 AACGcCGgca CGGGGCATTC CGcGCTGTGc GAATTGAACT AtgcgccGCT 201 GGGtgcggac ggcgtcatCA ATCCGGCGCg cgCCCTGAAT ATTGCCGAAC 251 AGTTTCATGT CAGCCGCCAG TTTTGGGcga cgctggTCGC GGAAGGCAAG 301 TTGGAAGACA ATTCCTTCAT CAATGCCGTG CCGCATATGT CTTTGGTGAT 351 GAACGAAGAC CACTGCCGTT ACCTGCAAAA ACGCTATGAT GTGTTTAAAA 401 CGCAGAAACT TTTTGAAAAT ATGGAATTTT CCACCGATCG GAACAAAATT 451 TCCGATTGGG CtccgCTGAT TATGCGCGGC CGGGACGAAA ACCAACCCGT 501 CGCCGCCAAC TATTCCGCCG AAGGCACGGA TGTCGATTTC GGACGGCTGA 551 CGCGCCAGAT GGTGAAATAT TTGCAGGGCA AGGGCGTAAA AACCGAGTTC 601 AACCGCCACG TCGAAGACAT CAAACGCGAA TCCGACGGCG CGTGGGTGCT 651 CAAAACCGCC GATACCCGCA ACCCAGACTG GCAGCTCACC CTCCGCACCC 701 GCTTCCTCTT CCTCGGCGCG GGCGGCGGCG CACTGACCCT GCTGCAAAAA 751 TCCGGCATCC CCGAAGGCAA AGGCTACGGC GGCTTACCCG TGTCCGGCCT 801 GTTCTTCCGC AACAGCAACC CCGAAACCGC CGAACAACAC AACGCCAAAG 851 TGTACGGGCA GGCTTCCGTC GGCGCGCCGC CGATGTCCGT CCCGCACCTC 901 GACACACGCA ACGTAGACGG CAAACGACAC CTTATGTTCG GTCCTTACGC 951 AGGTTTCCGT TCCAACTTCC TCAAGCAAGG CTCGTTTATG GATTTGCCGC 1001 TGTCCATCCA TATGGACAAC CTCTATCCTA TGCTGCGCGC CGGCTGGGCG 1051 AATATGCCGC TGACCAAATA CCTGCTGGGC GAATTGCGTA AAACCAAAGA 1101 AGAACGCTtt gCCTCCCTGC TGgaatacta cccGaggcag acccGACGAc 1151 tggtactcat cacgcaggnc acGCGTcata tcattanata tgactCgaaa 1201 ctgcgcgtgc tgcagttgta cgagattgtg ccaCGCGacg ctcgctcgcg 1251 cattctggag cgtcgcggcg catcacgctn tgcgctgata tccgctgatg 1301 acactgctcc gaGCGcgccc gtcttggaaa gtgtctga This corresponds to the amino acid sequence <SEQ ID 692; ORF 165-l.ng>: gl65-1. ep 1 MAEATDWLV GGGIMSATLG VLLKELEPSW EITLIERLED VALESSΝAWΝ 51 NAGTGHSALC ELNYAPLGAD GVINPARALN IAEQFHVSRQ FWATLVAEGK 101 LEDNSFINAV PHMSLVMNED HCRYLQKRYD VFKTQKLFEN MEFSTDRNKI 151 SDWAPLIMRG RDENQPVAAN YSAEGTDVDF GRLTRQMVKY LQGKGVKTEF 201 NRHVEDIKRE SDGAWVLKTA DTRNPDWQLT LRTRFLFLGA GGGALTLLQK 251 SGIPEGKGYG GLPVSGLFFR NSNPETAEQH NAKVYGQASV GAPPMSVPHL 301 DTRNVDGKRH LMFGPYAGFR SNFLKQGSFM DLPLSIHMDN LYPMLRAGWA 351 NMPLTKYLLG ELRKTKEERF ASLLEYYPRQ TRRLVLITQX TRHIIXYDΞK 401 LRVLQLYEIV PRDARΞRILE RRGASRXALI SADDTAPSAP VLESV* The following partial DNA sequence was identified in N meningitidis <SEQ ID 693>: ml65-l.seq 1 ATGGCTGAAG CGACAGACGT TGTCTTGGTG GGCGGCGGCA TTATGAGCGC 51 GACTTTGGGC GTTTTGCTCA AAGAACTCGA ACCGTCTTGG GAAATCACCC 101 TGATTGAACG CTTGGAAGAT GTGGCGTTGG AATCGTCAAA CGCGTGGAAC 151 AACGCCGGCA CGGGGCATTC CGCGCTGTGC GAATTGAACT ATGCGCCGTT 201 GGGTGCAAAT GGGATTATCG ATCCGGCGCG CGCCCTCAAT ATTGCCGAAC 251 AGTTTCATGT CAGCCGCCAG TTTTGGGCGA CGCTGGTCGC GGAAGGCAAG 301 TTGGAAGACA ATTCCTTCAT CAATGCCGTG CCGCATATGT CTTTGGTGAT 351 GAATGAAGAC CATTGTTCTT ATCTTCAAAA ACGTTATGAC GCGTTTAAAA 401 CCCAAAAACT TTTTGAAAAT ATGGAATTTT CCACCGATCG GAACAAAATT 451 TCCGATTGGG CTCCGCTGAT GATGCGCGGC CGGGACGAAA ACCAACCCGT 501 CGCCGCCAAC TACTCCGCCG AAGGTACGGA TGTCGATTTC GGACGGCTGA 551 CGCGCCAAAT GGTGAAATAT TTGCAGGGCA AGGGCGTAAA AACCGAGTTC 601 AACCGCCACG TCGAAGACAT CAAACGCGAA TCCGACGGCG CGTGGGTGCT 651 CAAAACCGCC GATACCCGCA ACCCCGACGG GCAGCTCACC CTCCGTACCC 701 GCTTCCTCTT CCTCGGCGCG GGCGGCGGCG CGCTGACCCT GCTGCAAAAA 751 TCCGGCATCC CCGAAGGCAA AGGCTACGGC GGCTTCCCCG TGTCCGGCCT 801 GTTCTTCCGC AACAGCAACC CCGAAACCGC CGAACAACAC AACGCCAAAG 851 TGTACGGGCA GGCTTCCGTC GGCGCGCCGC CGATGTCCGT CCCGCACCTC 901 GACACACGCA ACGTGGACGG CAAACGCCAC CTTATGTTCG GCCCTTACGC 951 AGGCTTCCGT TCCAACTTCC TCAAGCAAGG CTCGCTTATG GATTTGCCGC 1001 TGTCCATCCA TATGGACAAC CTCTATCCTA TGCTGTGCGC CGGCTGGGCG 1051 AATATGCCGC TGACCAAATA CCTGCTGGGC GAATTGCGTA AAACCAAAGA 1101 AGAACGCTTC GCCTCCCTGC TGGAATACTA CCCCGAGGCA AACCCCGACG 1151 ACTGGGAACT CATCACCGCA GGGCAACGCG TCCAAATCAT TAAAAAAGAC 1201 TCCGAAAAAG GCGGCGTGCT CCAGTTTGGT ACGGAGATTG TCGCCCACGC 1251 CGACGGCTCA CTCGCCGCAT TGCTGGGCGC GTCGCCGGGC GCATCGACCG 1301 CTGTGCCGCT GATGATCCGG CTGATGCACC AATGCTTCCC CGAGCGCGCC 1351 CCGTCTTGGG AAGACCGTCT GAAAGAGCTG GTACCGGGTT ACGGCATCAA 1401 GTTGAACGAA AACCCTGAAA GGGCGGATGA AATTATCGCC TATACCGCGA 1451 AAGTATTGGA TATTTAA This corresponds to the amino acid sequence <SEQ ID 694; ORF 165-1>: ml65-l.pep 1 MAEATDWLV GGGIMSATLG VLLKELEPSW EITLIERLED VALESSNAWN 51 NAGTGHSALC ELNYAPLGAN GIIDPARALN IAEQFHVSRQ FWATLVAEGK 101 LEDNSFINAV PHMSLVMNED HCSYLQKRYD AFKTQKLFEN MEFSTDRNKI 151 SDWAPLMMRG RDENQPVAAN YSAEGTDVDF GRLTRQMVKY LQGKGVKTEF 201 NRHVEDIKRE SDGAWVLKTA DTRNPDGQLT LRTRFLFLGA GGGALTLLQK 251 SGIPEGKGYG GFPVSGLFFR NSNPETAEQH NAKVYGQASV GAPPMSVPHL 301 DTRNVDGKRH LMFGPYAGFR SNFLKQGSLM DLPLSIHMDN LYPMLCAGWA 351 NMPLTKYLLG ELRKTKEERF ASLLEYYPEA NPDDWELITA GQRVQIIKKD 401 SEKGGVLQFG TEIVAHADGS LAALLGASPG AΞTAVPLMIR LMHQCFPERA 451 PSWEDRLKEL VPGYGIKLNE NPERADEIIA YTAKVLDI* ml65-l/gl65-l 89.7% identity in 428 aa overlap 10 20 30 40 50 60 ml65-1. pep MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALESΞNAWNNAGTGHΞALC I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl65-l MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALESSNAWNNAGTGHSALC 10 20 30 40 50 60 70 80 90 100 110 120 ml65-1. pep ELNYAPLGANGIIDPARALNIAEQFHVΞRQFWATLVAEGKLEDNΞFINAVPHMΞLVMNED I I I I I I I I I :| : I :| I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl65-l ELNYAPLGADGVINPARALNIAEQFHVSRQFWATLVAEGKLEDNSFINAVPHMSLVMNED 70 80 90 100 110 120 130 140 150 160 170 180 ml65-1. pep HCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRGRDENQPVAANYSAEGTDVDF II I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I: I I I I I I I I I I I I I I I I I I I I I I I gl65-l HCRYLQKRYDVFKTQKLFENMEFSTDRNKISDWAPLIMRGRDENQPVAANYSAEGTDVDF 130 140 150 160 170 180 190 200 210 220 230 240 ml65-1. pep GRLTRQMVKYLQGKGVKTEFNRHVEDIKREΞDGAWVLKTADTRNPDGQLTLRTRFLFLGA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl65-l GRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLKTADTRNPDWQLTLRTRFLFLGA 190 200 210 220 230 240 250 260 270 280 290 300 ml65-l.pep GGGALTLLQKΞGIPEGKGYGGFPVSGLFFRNSNPETAEQHNAKVYGQASVGAPPMSVPHL I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl65-l GGGALTLLQKSGIPEGKGYGGLPVΞGLFFRNSNPETAEQHNAKVYGQASVGAPPMSVPHL 250 260 270 280 290 300 310 320 330 340 350 360 ml65-1. pep DTRNVDGKRHLMFGPYAGFRSNFLKQGΞLMDLPLΞIHMDNLYPMLCAGWANMPLTKYLLG I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I gl65-l DTRNVDGKRHLMFGPYAGFRSNFLKQGSFMDLPLSIHMDNLYPMLRAGWANMPLTKYLLG 310 320 330 340 350 360 370 380 390 400 410 420 ml65-l.pep ELRKTKEERFASLLEYYPEANPDDWELITAGQRVQIIKKDSEKGGVLQFGTEIVAHADGS I I I I I I I I I I I I I I I I I I : : III I : I I II I III: : I : gl65-l ELRKTKEERFASLLEYYPR-QTRRLVLITQXTR-HIIXYDS-KLRVLQLYEIVPRDARSR 370 380 390 400 410 430 440 450 460 470 480 ml 65-1. pep LAALLGASPGASTAVPLMIRLMHQCFPERAPSWEDRLKELVPGYGIKLNENPERADEIIA : III gl65-l ILERRGASRXALISADDTAPΞAPVLESVX 420 430 440 The following partial DNA sequence was identified in N meningitidis <SEQ ID 695>: al65-l.seq 1 ATGGCTGAAG CGACAGACGT TGTCTTGGTG GGCGGCGGCA TTATGAGCGC 51 GACTTTGGGC GTTTTGCTCA AAGAACTCGA ACCGTCTTGG GAAATCACCC 101 TGATTGAACG CTTGGAAGAT GTGGCGTTGG AATCGTCAAA CGCGTGGAAC 151 AACGCCGGCA CGGGGCATTC CGCGCTGTGC GAATTGAACT ATGCGCCGTT 201 GGGTGCAAAT GGGATTATCG ATCCGGCGCG CGCCCTCAAT ATTGCCGAAC 251 AGTTTCATGT CAGCCGCCAG TTTTGGGCGA CGTTGGTCGC GGAAGGCAAG 301 TTGGAAGACA ATTCCTTCAT CAATGCCGTG CCGCATATGT CTTTGGTGAT 351 GAATGAAGAC CATTGTTCTT ATCTTCAAAA ACGTTATGAC GCGTTTAAAA 401 CCCAAAAACT TTTTGAAAAT ATGGAATTTT CCACCGATCG GAACAAAATT 451 TCCGATTGGG CTCCGCTGAT GATGCGCGGC CGGGACGAAA ACCAACCCGT 501 CGCCGCCAAC TACTCCGCCG AAGGCACGGA TGTCGATTTC GGACGGCTGA 551 CGCGCCAAAT GGTGAAATAT TTGCAGGGCA AGGGCGTAAA AACCGAGTTC 601 AACCGCCACG TCGAAGACAT CAAACGCGAA TCCGACGGCG CGTGGGTGCT 651 CAAAACCGCC GATACCCGCA ACCCCGACGG GCAGCTCACC CTCCGTACCC 701 GCTTCCTCTT CCTCGGCGCG GGCGGCGGCG CGCTGACCCT GCTGCAAAAA 751 TCCGGCATCC CCGAAGGCAA AGGCTACGGT GGCTTTCCCG TGTCCGGCCT 801 GTTCTTCCGC AACAGCAACC CCGAAACCGC CGAACAACAC AACGCCAAAG 851 TGTACGGGCA GGCTTCCGTC GGCGCGCCGC CGATGTCCGT CCCGCACCTC 901 GACACACGCA ACGTGGACGG CAAACGCCAC CTTATGTTCG GCCCTTACGC 951 AGGCTTCCGT TCCAACTTCC TCAAGCAAGG CTCACTTATG GATTTGCCGC 1001 TGTCCATCCA TATGGACAAC CTCTATCCTA TGCTGCGCGC CGGCTGGGCG 1051 AATATGCCGC TGACCAAATA CCTGCTGGGC GAATTGCGTA AAACCAAAGA 1101 AGAACGCTTC GCCTCCCTGC TGGAATACTA CCCCGAGGCA AACCCCGACG 1151 ACTGGGAACT CATCACCGCA GGGCAACGCG TTCAAATCAT TAAAAAAGAC 1201 TCCGAAAAAG GCGGCGTGTT GCAGTTTGGT ACGGAGATTG TCGCACACGC 1251 CGACGGCTCG CTCGCCGCAT TGCTGGGCGC GTCGCCGGGC GCATCGACCG 1301 CCGTGCCGCT GATGATCCGG CTGATGCACC AATGCTTCCC CGAACGCACC 1351 CCGTCTTGGG AAGGCCGTCT GAAAGAGCTG GTACCGGGTT ACGGCATCAA 1401 GTTGAACGAA AACCCCGAAA GGGCGGATGA AATTATCGCC TATACCGCGA 1451 AAGTGTTGGA TATTTAA This corresponds to the amino acid sequence <SEQ ID 696; ORF 165-l.a>: al65-l.pep 1 MAEATDWLV GGGIMSATLG VLLKELEPSW EITLIERLED VALESSNAWN 51 NAGTGHSALC ELNYAPLGAN GIIDPARALN IAEQFHVSRQ FWATLVAEGK 101 LEDNSFINAV PHMSLVMNED HCSYLQKRYD AFKTQKLFEN MEFSTDRNKI 151 SDWAPLMMRG RDENQPVAAN YSAEGTDVDF GRLTRQMVKY LQGKGVKTEF 201 NRHVEDIKRE SDGAWVLKTA DTRNPDGQLT LRTRFLFLGA GGGALTLLQK 251 SGIPEGKGYG GFPVSGLFFR NSNPETAEQH NAKVYGQASV GAPPMSVPHL 301 DTRNVDGKRH LMFGPYAGFR SNFLKQGSLM DLPLSIHMDN LYPMLRAGWA 351 NMPLTKYLLG ELRKTKEERF ASLLEYYPEA NPDDWELITA GQRVQIIKKD 401 SEKGGVLQFG TEIVAHADGS LAALLGASPG ASTAVPLMIR LMHQCFPERT 451 PSWEGRLKEL VPGYGIKLNE NPERADEIIA YTAKVLDI* al65-l/ l65-l 99.4% identity in 488 aa overlap 10 20 30 40 50 60 a165-1. pep MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALESSNAWNNAGTGHSALC I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml65-l MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALEΞSNAWNNAGTGHSALC 10 20 30 40 50 60 70 80 90 100 110 120 al65-l.pep ELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLEDNSFINAVPHMSLVMNED I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml65-1 ELNYAPLGANGIIDPARALNIAEQFHVΞRQFWATLVAEGKLEDNΞFINAVPHMΞLVMNED 70 80 90 100 110 120 130 140 150 160 170 180 al65-1.pep HCΞYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRGRDENQPVAANYΞAEGTDVDF I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I II I I I I I I I I I I I I I I I I II I I I I I I I I ml65-1 HCΞYLQKRYDAFKTQKLFENMEFSTDRNKIΞDWAPLMMRGRDENQPVAANYSAEGTDVDF 130 140 150 160 170 180 190 200 210 220 230 240 al65-l.pep GRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLKTADTRNPDGQLTLRTRFLFLGA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml65-1 GRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLKTADTRNPDGQLTLRTRFLFLGA 190 200 210 220 230 240 250 260 270 280 290 300 al65-l.pep GGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPETAEQHNAKVYGQASVGAPPMSVPHL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml65-1 GGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPETAEQHNAKVYGQASVGAPPMSVPHL 250 260 270 280 290 300 310 320 330 340 350 360 al65-l.pep DTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPLSIHMDNLYPMLRAGWANMPLTKYLLG I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml65-l DTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPLSIHMDNLYPMLCAGWANMPLTKYLLG 310 320 330 340 350 360 370 380 390 400 410 420 al65-l.pep ELRKTKEERFASLLEYYPEANPDDWELITAGQRVQIIKKDSEKGGVLQFGTEIVAHADGS I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml65-l ELRKTKEERFASLLEYYPEANPDDWELITAGQRVQIIKKDΞEKGGVLQFGTEIVAHADGS 370 380 390 400 410 420 430 440 450 460 470 480 al65-1. pep LAALLGASPGASTAVPLMIRLMHQCFPERTPSWEGRLKELVPGYGIKLNENPERADEIIA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ml65-1 LAALLGAΞPGASTAVPLMIRLMHQCFPERAPSWEDRLKELVPGYGIKLNENPERADEIIA 430 440 450 460 470 480 489 al65-l.pep YTAKVLDIX ml65-l YTAKVLDIX al65-l/p33940 sp|P33940|YOJH_ECOLI HYPOTHETICAL 60.2 KD PROTEIN IN ECO-ALKB INTERGENIC REGION >gι| 1736851|gnl|PID|dl016718 (D90850) ORF_ID:o372#5; similar to [SwissProt Accession Number P33940] [Escherichia coli] >gι 11788539 (AE000310) f548; This 548 aa ORF is 100 pet identical to 490 residues of YOJH_ECOLI ΞW: P33940 (492 aa) but contains 56 additional N-ter aa; 100 pet identical to GB: ECOHU49_33 ACCESSION: 000008 (490 aa) but contains 58 aditional N-term resi... Length = 548 Score = 458 bits (1167), Expect = e-128 Identities = 233/490 (47%), Positives = 303/490 (61%), Gaps = 5/490 (1%) Query: 3 EATDWLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALEΞSNAWNNAGTGHSALCEL 62 + TDV+L+GGGIMSATLG L+ELEP W +T++ERLE VA ESΞN WNNAGTGHSAL EL Sb 3 ct: 30 QETDVLLIGGGIMSATLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMEL 89 Query: 63 NYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLED-NSFINAVPHMSLVMNEDH 121 NY P A+G I +A+ I E F +SRQFWA V G L SFIN VPHMS V ED+ Sb 3 Ct: 90 NYTPQNADGSISIEKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDN 149 Query: 122 CΞYLQKRYDAFKTQKLFENMEFΞTDRNKISDWAPLMMRGRDENQPVAANYSAEGTDVDFG 181 ++L+ RY A + LF M +S D +1 +WAPL+M GRD Q VAA + GTDV++G Sb 3 ct: 150 VNFLRARYAALQQΞΞLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYG 209 Query: 182 RLTRQMVKYLQGKG-VKTEFNRHVEDIKRESDGAWVLKTADTRNPDGQLTXXXXXXXXXX 240 +TRQ++ LQ K + + V +KR D W + AD +N Q Sb 3 ct: 210 EITRQLIASLQKKSNFSLQLSΞEVRALKRNDDNTWTVTVADLKNGTAQ-NIRAKFVFIGA 268 Query: 241 XXXXXXXXQKSGIPEGKGYGGFPVSGLFFRNΞNPETAEQHNAKVYGQASVGAPPMSVPHL 300 Q+SGIPE K Y GFPV G F + NP+ H AKVYG+ASVGAPPMSVPH+ Sb 3 ct: 269 GGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKAΞVGAPPMSVPHI 328 Query: 301 DTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPLSIHMDNLYPMLRAGWANMPLTKYLLG 360 DTR +DGKR ++FGP+A F + FLK GΞL DL S N+ PM+ G N L KYL+ Sb]Ct: 329 DTRVLDGKRWLFGPFATFSTKFLKNGΞLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVS 388 Query: 361 ELRKTKEERFASLLEYYPEANPDDWELITAGQRVQIIKKDSEKGGVLQFGTEIVXXXXXX 420 ++ ++E+RF +L EYYP+A +DW L AGQRVQIIK+D+EKGGVL+ GTE+V ΞbJCt: 389 QVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEWSDQQGT 448 Query: 421 XXXXXXXXXXXXXXVPLMIRLMHQCFPER—TPSWEGRLKELVPGYGIKLNENPERADEI 478 P+M+ L+ + F +R +P W+ LK +VP YG KLN + + Sbjct: 449 IAALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE 506 Query: 479 IAYTAKVLDI 488 + YT++VL + Sbjct: 509 LQYTSEVLGL 518 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ED 697>: g204. seq 1 atggcggcgg cggaaataaa acgccccctc gctgtcgatt tccagcacat 51 agcgtccgtt ctgcacggcg gcatagccgc ttttgcctgc ctgatagggt 101 tgcagggcgg aatgcgaaat caggtaatca gtcagtttgc cgccgtcttc 151 ggcgatattg cccaccagtt tggcaaacaa ggtatggcac acgccgtttt 201 ccgcccagcc cgaaggcgcg tcctttccgt cggtttccat acatttgccg 251 acgacggctt ccaagtcgtt gggatgcttt ccggtcagcc ggacggcgtt 301 ttgttccggc aagcctttaa tcggataact gatttgtttt ttgccgtcgt 351 tggttttgcc ttcgctactt tgtcccaaag ccaaaccggc aatcgccgta 401 ttgtcgatgt atttgacttt gaaaaccggt ttcggcgcgc tttgtgccgc 451 attttgcggc tgttccgccg tattttcgga tttgccgcag gcggcaagca 501 gcaggcagcc gcccaacacg gcaaaaggta ttttcagcat tccgcactcc 551 tgatggtttc aaaatgccgt ctgaaatgcc gtctgaaacg tggcaggcgg 601 aggttcggac ggcattgggt ttatttcaac gggcggatgc cgaccgcatc 651 gcgtacttta tccaacaatt cgcgcgcttc tttgcgcgct ttttgcgcgc 701 ctgcctgcaa aatctcttcg atttgcgaag gattagaggt caatgcgttg 751 tag This corresponds to the amino acid sequence <SEQ ID 698; ORF 204.ng>: g204.pep 1 MAAAEIKRPL AVDFQHIASV LHGGIAAFAC LIGLQGGMRΝ QVISQFAAVF 51 GDIAHQFGKQ GMAHAVFRPA RRRVLSVGFH TFADDGFQW GMLSGQPDGV 101 LFRQAFΝRIT DLFFAWGFA FATLSQSQTG ΝRRIVDVFDF EΝRFRRALCR 151 ILRLFRRIFG FAAGGKQQAA AQHGKRYFQH SALLMVSKCR LKCRLKRGRR 201 RFGRHWVYFΝ GRMPTASRTL SΝΝSRASLRA FCAPACKISS ICEGLEVΝAL 251 * The following partial DΝA sequence was identified in N meningitidis <SEQ ID 699>: m204. seq 1 ATGGCGGCGG CGGAAATAAA ACGCCCCTTC GCTGTCGATT TCCAGCACAT 51 AGCGTCCGTT CTGCACGGCG GCATAGCCGC TTTTGCCTGC CTGATAGGGT 101 TGCAGGGCGG CATGCGAAAC TAGGTAATCC GTCAGTTTGC CGCCGTCTTC 151 GGCGATATTG CCCACCAGTT TGGCAAACAA GGTATGGCAC ACGCCGTTTT 201 CTGCCCAACC TGCCGGACTG TCCTTATCAT CGGTTTCCAT ACATTTGCCG 251 CTGACGGCTT CCAAGTCGCC GGGATGCTTG CCGATCAGTC GGATAACATT 301 TTGTTCCGGC AAGCCTTTAA TCGGATAACT GATTTGTTTT TTGCCGTCGT 351 TGGTTTTGCC TTCGCTGCTT TGTCCCAAAT CCAAACCGGC AATCGCCGTA 401 TTGTCGATAT ATATGACTTT GAAAACCGGT TTCGGCGCGC TTTGTACCGC 451 GTTTTGCGGC TGTACCGCCG TATTTwCGGA TTTGCCGCaC GGCaArGCAG 501 CAGGCAGCCG CCCAATACGG CAAAArAwGT TTCAGCATT CCACAyTCCT 551 GATGGTTTCA AAATGCCGTC TGAAACGCGG CAGGCGGAGG TTCGGACGGC 601 ATCGGGTTCA TTTCAACGGG CGGATGcCGA CCGCATCgGT ACTTTGTCCA 651 ATAATTCGCG TGCTTCTTTA CGCGCTTTCG CCGCGCCTGC CTGCAAAATC 701 TCTTCGATTT GCGAAGGGTC GGCGGTCAGC TCGTTGTAG This corresponds to the amino acid sequence <SEQ ID 700; ORF 204>: m20 .pep 1 MAAAEIKRPF AVDFQHIASV LHGGIAAFAC LIGLQGGMRΝ *VIRQFAAVF 51 GDIAHQFGKQ GMAHAVFCPT CRTVLIIGFH TFAADGFQVA GMLADQSDΝI 101 LFRQAFΝRIT DLFFAWGFA FAALSQIQTG ΝRRIVDIYDF EΝRFRRALYR 151 VLRLYRRIXG FAATAXQQAA AQYGKXXXQH STXLMVSKCR LKRGRRRFGR 201 HRVHFΝGRMP TASGTLSΝΝS RASLRAFAAP ACKISSICEG SAVSSL* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 204 shows 82.0% identity over a 250 aa overlap with a predicted ORF (ORF 204.ng) from N gonorrhoeae: m204/g204 10 20 30 40 50 60 m204.pep MAAAEIKRPFAVDFQHIASVLHGGIAAFACLIGLQGGMRΝXVIRQFAAVFGDIAHQFGKQ MIMMIMMMMMMIMMMMMIMMMM II 1111111111111111 g204 MAAAEIKRPLAVDFQHIASVLHGGIAAFACLIGLQGGMRΝQVISQFAAVFGDIAHQFGKQ 10 20 30 40 50 60 70 80 90 100 110 120 m204.pep GMAHAVFCPTCRTVLIIGFHTFAADGFQVAGMLADQSDΝILFRQAFΝRITDLFFAWGFA I II MllhllM I | : g204 GMAHAVFRPARRRVLSVGFHTFADDGFQWGMLSGQPDGVLFRQAFΝRITDLFFAWGFA 70 80 90 100 110 120 130 140 150 160 170 180 m204. pep FAALSQIQTGΝRRIVDIYDFEΝRFRRALYRVLRLYRRIXGFAATAXQQAAAQYGKXXXQH I lll MMMMhMMIMMM ll lll MM = lllllhll II g204 FATLSQSQTGΝRRIVDVFDFEΝRFRRALCRILRLFRRIFGFAAGGKQQAAAQHGKRYFQH 130 140 150 160 170 180 190 200 210 220 230 m204. pep STXLMVSKCRLK RGRRRFGRHRVHFΝGRMPTASGTLSΝΝSRASLRAFAAPACKISS h g204 SALLMVSKCRLKCRLKRGRRRFGRHWVYFΝGRMPTASRTLSΝΝSRASLRAFCAPACKISS 190 200 210 220 230 240 240 m20 .pep ICEGSAVSSLX 1 1111111 11 -• ■• 11 g204 ICEGLEVΝAL 250 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 701>: a204.seq 1 ATGGCGGCGG CGGAAATAAA ACGCCCCCTC GCTGTCGATT TCCAGCACAT 51 AGCGTCCGTT CTGCACGGCG GCATAGCCGC TTTTGCCTGC CTGATAGGGT 101 TGCAGGGCGG AATGCGAAAT CAGGTAATCC GTCAGTTTGC CGCCGTCTTC 151 GGCGATATTG CCCACCAGTT TGGCAAACAA GGTATGGCAC ACGCCGTTTG 201 CCGCCCAGCC CGAAGGCGCG CCCTTTCCGT CGGTTTCCAT ACATTTGCCG 251 ACGACGGCTT CCAAGTCGTT GGGATGCTTG CCGGTCAGCC GGACGACGTT 301 TTGTTCCGGC AAGCCTTT 351 401 451 501 551 AAGAG GTTCGGACGG 601 CATTGGGTTT ATTTCAACGG GCGGATACCG ACCGCATCAC GTACTTTGCC 651 CAATAATTCG CGTGCTTCTT TACGCGCTTT TTGCGCGCCT GCCTGCAAAA 701 TCTCTTCGAT TTGCGAAGGG TCGGCGGTCA GCTCGTTGTA G This corresponds to the amino acid sequence <SEQ ID 702; ORF 204.a>: 1 MAAAEIKRPL AVDFQHIASV LHGGIAAFAC LIGLQGGMRΝ QVIRQFAAVF 51 GDIAHQFGKQ GMAHAVCRPA RRRALSVGFH TFADDGFQW GMLAGQPDDV 101 LFRQAF 151 KRFGR 201 HWVYFΝGRIP TASRTLPΝΝS RASLRAFCAP ACKISSICEG SAVSSL* m204/a204 54.5% identity in 246 aa overlap 10 20 30 40 50 60 m204. pep MAAAEIKRPFAVDFQHIASVLHGGIAAFACLIGLQGGMRNXVIRQFAAVFGDIAHQFGKQ I I I I I I I I 1:1 I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a204 MAAAEIKRPLAVDFQHIASVLHGGIAAFACLIGLQGGMRNQVIRQFAAVFGDIAHQFGKQ 10 20 30 40 50 60 70 80 90 100 110 120 m20 . pep GMAHAVFCPTCRTVLIIGFHTFAADGFQVAGMLADQΞDNILFRQAFNRITDLFFAWGFA l l l l l l I : I : :| I I I I I I I I I I :| I I I I |:: I I I I I I a204 GMAHAVCRPARRRALSVGFHTFADDGFQWGMLAGQPDDVLFRQAF 70 80 90 100 130 140 150 160 170 180 m204. pep FAALSQIQTGNRRIVDIYDFENRFRRALYRVLRLYRRIXGFAATAXQQAAAQYGKXXXQH a204 190 200 210 220 230 240 m20 .pep STXLMVΞKCRLKRGRRRFGRHRVHFNGRMPTASGTLSNNSRASLRAFAAPACKIΞΞICEG :| I I I I I : I I I I :| I I I II I I I I I I I I I I I I I I I I I I I I I I a204 KRFGRHWVYFNGRIPTASRTLPNNSRASLRAFCAPACKISΞICEG 110 120 130 140 150 m204.pep SAVSSLX lllllll a204 SAVSSLX The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 703>: g205.seq 1 atgctgaaaa taccttttgc cgtgttgggc ggctgcctgc tgcttgccgc 51 ctgcggcaaa tccgaaaata cggcggaaca gccgcaaaat gcggcacaaa 101 gcgcgccgaa accggttttc aaagtcaaat acatcgacaa tacggcgatt 151 gccggtttgg ctttgggaca aagtagcgaa ggcaaaacca acgacggcaa 201 aaaacaaatc agttatccga ttaaaggctt gccggaacaa aacgccgtcc 251 ggctgaccgg aaagcatccc aacgacttgg aagccgtcgt cggcaaatgt 301 atggaaaccg acggaaagga cgcgccttcg ggctgggcgg aaaacggcgt 351 gtgccatacc ttgtttgcca aactggtggg caatatcgcc gaagacggcg 401 gcaaactgac tgattacctg atttcgcatt ccgccctgca accctatcag 451 gcaggcaaaa gcggctatgc cgccgtgcag aacggacgct atgtgctgga 501 aatcgacagc gagggggcgt tttatttccg ccgccgccat tattga This corresponds to the amino acid sequence <SEQ ID 704; ORF 205.ng>: g205.pep 1 MLKIPFAVLG GCLLLAACGK SEΝTAEQPQΝ AAQSAPKPVF KVKYIDΝTAI 51 AGLALGQSSE GKTΝDGKKQI SYPIKGLPEQ ΝAVRLTGKHP ΝDLEAWGKC 101 METDGKDAPS GWAEΝGVCHT LFAKLVGΝIA EDGGKLTDYL ISHSALQPYQ 151 AGKSGYAAVQ ΝGRYVLEIDS EGAFYFRRRH Y The following partial DΝA sequence was identified in N meningitidis <SEQ ID 705>: m205.seq 1 ATGCTGAAwA CwTyTTTTGC CGTATTGGGC GGCTGCCTGC TGCyTtGCCG 51 tGCGGCAAAT CCG AAATAC GGCGGTACAG CCGCAAAACG CGGTACAAAG 101 CGCGCCGAAA CCGGTTTTCA AAGTCATATA TATCGACAAT ACGGCGATTG 151 CCGGTTTGGA TTTGGGACAA AGCAGCGAAG GCAAAACCAA CGACGGCAAA 201 AAACAAATCA GTTATCCGAT TAAAGGCTTG CCGGAACAAA ATGTTATCCG 251 ACTGATCGGC AAGCATCCCG GCGACTTGGA AGCCGTCAGC GGCAAATGTA 301 TGGAAACCGA TGATAAGGAC AGTCCGGCAG GTTGGGCAGA AAACGGCGTG 351 TGCCATACCT TGTTTGCCAA ACTGGTGGGC AATATCGCCG AAGACGGCGG 401 CAAACTGACG GATTACCTAG TTTCGCATGC CGCCCTGCAA CCCTATCAGG 451 CAGGCAAAAG CGGCTATGCC GCCGTGCAGA ACGGACGCTA TGTGCTGGAA 501 ATCGACAGCG AAGGGGCGTT TTATTTCCGC CGCCGCCATT ATTGA This corresponds to the amino acid sequence <SEQ ID 706; ORF 205>: m 05.pep 1 MLXTXFAVLG GCLLXCRCGK SXNTAVQPQN AVQSAPKPVF KVIYIDNTAI 51 AGLDLGQSSE GKTNDGKKQI SYPIKGLPEQ NVIRLIGKHP GDLEAVSGKC 101 METDDKDSPA GWAENGVCHT LFAKLVGNIA EDGGKLTDYL VSHAALQPYQ 151 AGKSGYAAVQ NGRYVLEIDS EGAFYFRRRH Y* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 205 shows 88.4% identity over a 181 aa overlap with a predicted ORF (ORF 205.ng) from N gonorrhoeae: m205/g205 10 20 30 40 50 60 m205. pep MLXTXFAVLGGCLLXCRCGKSXΝTAVQPQΝAVQSAPKPVFKVIYIDΝTAIAGLDLGQSSE II ll l ll ll l l M M I I I M M MMM M MM i l l l l ll ll g205 MLKIPFAVLGGCLLLAACGKSEΝTAEQPQΝAAQSAPKPVFKVKYIDΝTAIAGLALGQSSE 10 20 30 40 50 60 70 80 90 100 110 120 m205. pep GKTΝDGKKQISYPIKGLPEQΝVIRLIGKHPGDLEAVSGKCMETDDKDSPAGWAEΝGVCHT M MMM M M MM M MMM I ll lhll ll l l l ll l l l IhMMMM M M g205 GKTΝDGKKQISYPIKGLPEQΝAVRLTGKHPΝDLEAWGKCMETDGKDAPSGWAEΝGVCHT 70 80 90 100 110 120 130 140 150 160 170 180 m205.pep LFAKLVGΝIAEDGGKLTDYLVSHAALQPYQAGKSGYAAVQΝGRYVLEIDSEGAFYFRRRH MIMMMMMMIMIhlMMMMMMMMMMMMMMMMMMM g205 LFAKLVGΝIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQΝGRYVLEIDSEGAFYFRRRH 130 140 150 160 170 180 m205.pep YX I g205 Y The following partial DΝA sequence was identified in N meningitidis <SEQ ID 707>: a205.seq (partial) 1 TCCGAACCTC TTAAAGGCTT GCCGGAACAA AACGTCGTCC GGCTGACCGG 51 CAAGCATCCC AACGACTTGG AAGCCGTCGT CGGCAAATGT ATGGAAACCG 101 ACGGAAAGGG CGCGCCTTCG GGCTGGGCGG CAAACGGCGT GTGCCATACC 151 TTGTTTGCCA AACTGGTGGG CAATATCGCC GAAGACGGCG GCAAACTGAC 201 GGATTACCTG ATTTCGCATT CCGCCCTGCA ACCCTATCAG GCAGGCAAAA 251 GCGGCTATGC CGCCGTGCAG AACGGACGCT ATGTGCTGGA AATCGACAGC 301 GAGGGGGCGT TTTATTTCCG CCGCCGCCAT TATTGA This corresponds to the amino acid sequence <SEQ ID 708; ORF 205. a>: a205.pep (partial) 1 SEPLKGLPEQ ΝWRLTGKHP ΝDLEAVVGKC METDGKGAPS GWAAΝGVCHT 51 LFAKLVGNIA EDGGKLTDYL ISHSALQPYQ AGKSGYAAVQ NGRYVLEIDS 101 EGAFYFRRRH Y* m205/a205 88.3% identity in 111 aa overlap 50 60 70 80 90 100 m205.pep KVIYIDNTAIAGLDLGQSSEGKTNDGKKQISYPIKGLPEQNVIRLIGKHPGDLEAVSGKC I I : I I I I I I I I : I I l l l l l l l l I I I a205 SEPLKGLPEQNWRLTGKHPNDLEAWGKC 10 20 30 110 120 130 140 150 160 m205.pep METDDKDSPAGWAENGVCHTLFAKLVGNIAEDGGKLTDYLVSHAALQPYQAGKSGYAAVQ I I I I I : I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I : I I I I I I I I I I I I I I I I a205 METDGKGAPSGWAANGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQ 40 50 60 70 80 90 170 180 m205. pep NGRYVLEIDSEGAFYFRRRHYX I II I I I I I I I I I I I I I I I I I I I a205 NGRYVLEIDSEGAFYFRRRHYX 100 110 The following partial DNA sequence was identified in N gonorrhoeae <SEQ LD 709>: g205-l.seq (partial) 1 ATGCTGAAAA TAcCTTTTGC CGTGTTGGGC GGCTGCCTGC TGCTTGCCGC 51 CTGCGGCAAA TCCGAAAATA CGGCGGAACA GCCGCAAAAT GCGGCACAAA 101 GCGCGCCGAA ACCGGTTTTC AAAGTCAAAT ACATCGACAA TACGGCGATT 151 GCCGGTTTGG CTTTGGGACA AAGTAGCGAA GGCAAAACCA ACGACGGCAA 201 AAAACAAATC AGTTATCCGA TTAAAGGCTT GCCGGAACAA AACGCCGTCC 251 GGCTGACCGG AAAGCATCCC AACGACTTGG AAGCCGTCGT CGGCAAATGT 301 ATGGAAACCG ACGGAAAGGA CGCGCCTTCG GGCTGGGCGG AAAACGGCGT 351 GTGCCATACC TTGTTTGCCA AACTGGTGGG CAATATCGCC GAAGACGGCG 401 GCAAACTGAC TGATTACCTG ATTTCGCATT CCGCCCTGCA ACCCTATCAG 451 GCAGGCAAAA GCGGCTATGC CGCCGTGCAG AACGGACGCT ATGTGCTGGA 501 AATCGACAGC GAGGGGGCGT TTTA This corresponds to the amino acid sequence <SEQ ID 710; ORF 205-l.ng>: g205-l.pep (partial) . 1 MLKIPFAVLG GCLLLAACGK SEΝTAEQPQΝ AAQSAPKPVF KVKYIDΝTAI 51 AGLALGQSSE GKTΝDGKKQI SYPIKGLPEQ ΝAVRLTGKHP ΝDLEA GKC 101 METDGKDAPS GWAEΝGVCHT LFAKLVGNIA EDGGKLTDYL ISHSALQPYQ 151 AGKSGYAAVQ NGRYVLEIDS EGAF The following partial DNA sequence was identified in N meningitidis <SEQ ED 711>: m205-l.seq.. 1 ATGCTGAAAA CATCTTTTGC CGTATTGGGC GGCTGCCTGC TGCTTGCCGC 51 CTGCGGCAAA TCCGAAAATA CGGCGGAACA GCCGCAAAAC GCGGTACAAA 101 GCGCGCCGAA ACCGGTTTTC AAAGTCAAAT ATATCGACAA TACGGCGATT 151 GCCGGTTTGG ATTTGGGACA AAGCAGCGAA GGCAAAACCA ACGACGGCAA 201 AAAACAAATC AGTTATCCGA TTAAAGGCTT GCCGGAACAA AATGTTATCC 251 GACTGATCGG CAAGCATCCC GGCGACTTGG AAGCCGTCAG CGGCAAATGT 301 ATGGAAACCG ATGATAAGGA CAGTCCGGCA GGTTGGGCAG AAAACGGCGT 351 GTGCCATACC TTGTTTGCCA AACTGGTGGG CAATATCGCC GAAGACGGCG 401 GCAAACTGAC GGATTACCTA GTTTCGCATG CCGCCCTGCA ACCCTATCAG 451 GCAGGCAAAA GCGGCTATGC CGCCGTGCAG AACGGACGCT ATGTGCTGGA 501 AATCGACAGC GAAGGGGCGT TTTATTTCCG CCGCCGCCAT TATTGA This corresponds to the amino acid sequence <SEQ ID 712; ORF 205-l>: m205-l.pep 1 MLKTSFAVLG GCLLLAACGK SEΝTAEQPQΝ AVQSAPKPVF KVKYIDΝTAI 51 AGLDLGQSSE GKTΝDGKKQI SYPIKGLPEQ ΝVIRLIGKHP GDLEAVSGKC 101 METDDKDSPA GWAEΝGVCHT LFAKLVGNIA EDGGKLTDYL VSHAALQPYQ 151 AGKSGYAAVQ NGRYVLEIDS EGAFYFRRRH Y* 205-l/g205-l 92.0% identity in 174 aa overlap 10 20 30 40 50 60 g205-1.pep MLKIPFAVLGGCLLLAACGKSENTAEQPQNAAQSAPKPVFKVKYIDNTAIAGLALGQSSE I I I m205 - l MLKTSFAVLGGCLLLAACGKSENTAEQPQNAVQSAPKPVFKVKYIDNTAIAGLDLGQSSE 10 20 30 40 50 60 70 80 90 100 110 120 g205 - l . pep GKTNDGKKQISYPIKGLPEQNAVRLTGKHPNDLEAWGKCMETDGKDAPSGWAENGVCHT : I I I I M i I I I I - l l l h l l l l l m205 - 1 GKTNDGKKQISYPIKGLPEQNVIRLIGKHPGDLEAVSGKCMETDDKDSPAGWAENGVCHT 70 80 90 100 110 120 130 140 150 160 170 g205-l .pep LFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQNGRYVLEIDSEGAF Itl205-1 LFAKLVGNIAEDGGKLTDYLVSHAALQPYQAGKSGYAAVQNGRYVLEIDSEGAFYFRRRH 130 140 150 160 170 180 m205-l YX The following partial DNA sequence was identified in N meningitidis <SEQ ID 713>: a205-l.seq (partial) 1 CCTCTTAAAG GCTTGCCGGA ACAAAACGTC GTCCGGCTGA CCGGCAAGCA 51 TCCCAACGAC TTGGAAGCCG TCGTCGGCAA ATGTATGGAA ACCGACGGAA 101 AGGGCGCGCC TTCGGGCTGG GCGGCAAACG GCGTGTGCCA TACCTTGTTT 151 GCCAAACTGG TGGGCAATAT CGCCGAAGAC GGCGGCAAAC TGACGGATTA 201 CCTGATTTCG CATTCCGCCC TGCAACCCTA TCAGGCAGGC AAAAGCGGCT 251 ATGCCGCCGT GCAGAACGGA CGCTATGTGC TGGAAATCGA CAGCGAGGGG 301 GCGTTTTATT TCCGCCGCCG CCATTATTGA This corresponds to the amino acid sequence <SEQ ID 714; ORF 205-1. a>: a205-l.pep (partial) 1 PLKGLPEQΝV VRLTGKHPΝD LEAWGKCME TDGKGAPSGW AAΝGVCHTLF 51 AKLVGΝIAED GGKLTDYLIS HSALQPYQAG KSGYAAVQΝG RYVLEIDSEG 101 AFYFRRRHY* m205-l/a205-l 89.0% identity in 109 aa overlap 50 60 70 80 90 100 m205-l.pep KYIDΝTAIAGLDLGQSSEGKTΝDGKKQISYPIKGLPEQΝVIRLIGKHPGDLEAVSGKCME h l l l l l l l h l l l l l h l l l l l M i l l a205-l PLKGLPEQΝWRLTGKHPΝDLEAWGKCME 10 20 30 110 120 130 140 150 160 m205-1. pep TDDKDSPAGWAEΝGVCHTLFAKLVGΝIAEDGGKLTDYLVSHAALQPYQAGKSGYAAVQΝG II I lll a205-l TDGKGAPSGWAAΝGVCHTLFAKLVGΝIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQΝG 40 50 60 70 80 90 170 180 m205 -1. pep RYVLEIDSEGAFYFRRRHYX a205-l RYVLEIDSEGAFYFRRRHYX 100 110 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ED 715>: g206. seq 1 atgttttccc ccgacaaaac ccttttcctc tgtctcggcg cactgctcct 51 cgcctcatgc ggcacgacct ccggcaaaca ccgccaaccg aaacccaaac 101 agacagtccg gcaaatccaa gccgtccgca tcagccacat cggccgcaca 151 caaggctcgc aggaactcat gctccacagc ctcggactca tcggcacgcc 201 ctacaaatgg ggcggcagca gcaccgcaac cggcttcgac tgcagcggca 251 tgattcaatt ggtttacaaa aacgccctca acgtcaagct gccgcgcacc 301 gcccgcgaca tggcggcggc aagccgcaaa atccccgaca gccgcctcaa 351 ggccggcgac atcgtattct tcaacaccgg cggcgcacac cgctactcac 401 acgtcggact ctacatcggc aacggcgaat tcatccatgc ccccggcagc 451 ggcaaaacca tcaaaaccga aaaactctcc acaccgtttt acgccaaaaa 501 ctaccttgga gcgcatacgt tttttacaga atga This corresponds to the amino acid sequence <SEQ ID 716; ORF 206.ng>: g206.pep 1 MFSPDKTLFL CLGALLLASC GTTSGKHRQP KPKQTVRQIQ AVRISHIGRT 51 QGSQELMLHS LGLIGTPYKW GGSSTATGFD CSGMIQLVYK NALNVKLPRT 101 ARDMAAASRK IPDSRLKAGD IVFFNTGGAH RYSHVGLYIG NGEFIHAPGS 151 GKTIKTEKLS TPFYAKNYLG AHTFFTE* The following partial DNA sequence was identified in N meningitidis <SEQ ID 717>: m206.seq 1 ATGTTTCCCC CCGACAAAAC CCTTTTCCTC TGTCTCAGCG CACTGCTCCT 51 CGCCTCATGC GGCACGACCT CCGGCAAACA CCGCCAACCG AAACCCAAAC 101 AGACAGTCCG GCAAATCCAA GCCGTCCGCA TCAGCCACAT CGACCGCACA 151 CAAGGCTCGC AGGAACTCAT GCTCCACAGC CTCGGACTCA TCGGCACGCC 201 CTACAAATGG GGCGGCAGCA GCACCGCAAC CGGCTTCGAT TGCAGCGGCA 251 TGATTCAATT CGTTTACAAr AACGCCCTCA ACGTCAAGCT GCCGCGCACC 301 GCCCGCGACA TGGCGGCGGC AAGCCGsAAA ATCCCCGAcA GCCGCyTCAA 351 GGCCGGCGAC CTCGTATTCT TCAACACCGG CGGCGCACAC CGCTACTCAC 401 ACGTCGGACT CTACATCGGC AACGGCGAAT TCATCCATGC CCCCAGCAGC 451 GGCAAAACCA TCAAAACCGA AAAACTCTCC ACACCGTTTT ACGCCAAAAA 501 CTACCTCGGC GCACATACTT TTTTTACAGA ATGA This corresponds to the amino acid sequence <SEQ ID 718; ORF 206>: m206.pep.. 1 MFPPDKTLFL CLSALLLASC GTTSGKHRQP KPKQTVRQIQ AVRISHIDRT 51 QGSQELMLHS LGLIGTPYKW GGSSTATGFD CSGMIQFVYK ΝALΝVKLPRT 101 ARDMAAASRK IPDSRXKAGD LVFFΝTGGAH RYSHVGLYIG ΝGEFIHAPSS 151 GKTIKTEKLS TPFYAKNYLG AHTFFTE* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 206 shows 96.0% identity over a 177 aa overlap with a predicted ORF (ORF 206.ng) from N gonorrhoeae: m206/g206 10 20 30 40 50 60 m206.pep MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRISHIDRTQGSQELMLHS I I l l l l l l l l h l l l l l l l l l l l l l l l l l l l l l l l l l l l l l l l l l l l l l l l l l l l l l l g206 MFSPDKTLFLCLGALLLASCGTTSGKHRQPKPKQTVRQIQAVRISHIGRTQGSQELMLHS 10 20 30 40 50 60 70 80 90 100 110 120 m206.pep LGLIGTPYKWGGSSTATGFDCSGMIQFVYKΝALΝVKLPRTARDMAAASRKIPDSRXKAGD MM g206 LGLIGTPYKWGGSSTATGFDCSGMIQLVYKΝALΝVKLPRTARDMAAASRKIPDSRLKAGD 70 80 90 100 110 120 130 140 150 160 170 m206.pep LVFFΝTGGAHRYSHVGLYIGΝGEFIHAPSSGKTIKTEKLSTPFYAKΝYLGAHTFFTEX g206 IVFFΝTGGAHRYSHVGLYIGΝGEFIHAPGSGKTIKTEKLSTPFYAKΝYLGAHTFFTE 130 140 150 160 170 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 719>: a206. seq 1 ATGTTTCCCC CCGACAAAAC CCTTTTCCTC TGTCTCAGCG CACTGCTCCT 51 CGCCTCATGC GGCACGACCT CCGGCAAACA CCGCCAACCG AAACCCAAAC 101 AGACAGTCCG GCAAATCCAA GCCGTCCGCA TCAGCCACAT CGACCGCACA 151 CAAGGCTCGC AGGAACTCAT GCTCCACAGC CTCGGACTCA TCGGCACGCC 201 CTACAAATGG GGCGGCAGCA GCACCGCAAC CGGCTTCGAT TGCAGCGGCA 251 TGATTCAATT CGTTTACAAA AACGCCCTCA ACGTCAAGCT GCCGCGCACC 301 GCCCGCGACA TGGCGGCGGC AAGCCGCAAA ATCCCCGACA GCCGCCTTAA 351 GGCCGGCGAC CTCGTATTCT TCAACACCGG CGGCGCACAC CGCTACTCAC 401 ACGTCGGACT CTATATCGGC AACGGCGAAT TCATCCATGC CCCCAGCAGC 451 GGCAAAACCA TCAAAACCGA AAAACTCTCC ACACCGTTTT ACGCCAAAAA 501 CTACCTCGGC GCACATACTT TCTTTACAGA ATGA This corresponds to the amino acid sequence <SEQ ID 720; ORF 206.a>: a206.pep 1 MFPPDKTLFL CLSALLLASC GTTSGKHRQP KPKQTVRQIQ AVRISHIDRT 51 QGSQELMLHS LGLIGTPYKW GGSSTATGFD CSGMIQFVYK NALNVKLPRT 101 ARDMAAASRK IPDSRLKAGD LVFFNTGGAH RYSHVGLYIG NGEFIHAPSS 151 GKTIKTEKLS TPFYAKNYLG AHTFFTE* m206/a206 99.4% identity in 177 aa overlap 10 20 30 40 50 60 m206.pep MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRISHIDRTQGSQELMLHS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a206 MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRISHIDRTQGSQELMLHS 10 20 30 40 50 60 70 80 90 100 110 120 m206.pep LGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTARDMAAASRKIPDSRXKAGD I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I II I I I I I I a206 LGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTARDMAAASRKIPDSRLKAGD 70 80 90 100 110 120 130 140 150 160 170 m206. pep LVFFNTGGAHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLSTPFYAKNYLGAHTFFTEX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a206 LVFFNTGGAHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLSTPFYAKNYLGAHTFFTEX 130 140 150 160 170 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 721>: g209.seq 1 atgctgcggc atttaggaaa cgacttcgcc ttgggcgcgt tgtttttcga 51 tgctgcggtt gatgtgccac tgctgggcga tggtcaggag gttgttgacc 101 acccagtaga gaaccaaacc ggcagggaag aagaagaaca tgacggagaa 151 aaccaacggc atgattttca tcattttcgc ctgcatcggg tcggtcggcg 201 gcgggttcag ataggtttgg gcgaacatcg ttgccgccat aatgatgggc 251 aggatgtagt aggggtcggc gcggctgagg tcggtaatcc agcccagcca 301 aggtgcctgg cgcaattcta cggaggcgaa caatgcccag tacaagccga 351 tgaagacggg gatttgcaac agcataggca gacagccgcc cagcgggttg 401 atttcctcgt cttcgaaaag ctgcatcatc gcttgctgtt gcgccatacg 451 gtcgtcgccg tatttttctt tgatggtctg cagttcgggt gcggcggcac 501 gcattttcgc catcgaacgg taggaggcgt tggtcaatgg atacagtacg 551 gctttgacga tgatggtcaa aacgacgatt gcccagcccc agttgccgat 601 aatgttgtgc agttggttca ggagccagaa gagcggcgat gcgaaccagt 651 gtactttacc gtagtctttt gccagttgca ggttgtcggc gatgtttgcg 701 ataacggatg tggtttgcgg accggcatac aggttgaccg ccattttcgg 751 ttttggcccc cgggttggga tagcggttaa This corresponds to the amino acid sequence <SEQ ID 722; ORF 209.ng>: g209.pep 1 MLRHLGΝDFA LGALFFDAAV DVPLLGDGQE DHPVEΝQT GREEEEHDGE 51 ΝQRHDFHHFR LHRVGRRRVQ IGLGEHRCRH ΝDGQDWGVG AAEVGΝPAQP 101 RCLAQFYGGE QCPVQADEDG DLQQHRQTAA QRVDFLVFEK LHHRLLLRHT 151 WAVFFFDGL QFGCGGTHFR HRTVGGVGQW IQYGFDDDGQ ΝDDCPAPVAD 201 ΝWQLVQEPE ERRCEPVYFT WFCQLQWG DVCDΝGCGLR TGIQVDRHFR 251 FWPPGWDSG* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 723>: m209.seq 1 ATGCTGCGGC ATTTAGGAAA CGACTTCGCC TTGGgGCGTT GTTTTTCGAT 51 GCTGCGGTTG ATGTGCCATT GCTGGGCGAT GGTCAGGAGG TTGTTGACTA 101 CCCAGTACAA TACCAGACCG GCAGGGAAGA AGAAGAACAT GACGGAGAAA 151 ACCAACGGCA TGATTTTCAT CATTTTCGCC TGCATCGGGT CGGTCGGCGG 201 CGGGTTCAGA TAAGTTTGGG CGAACATCGT TGCCGCCATA ATGATGGGCA 251 GGATGTAGTA GGGGTCGGCG CGGCTGAGGT CGGTAATCCA ACCCAGCCAA 301 GGTGCCTGGC GCAATTCTAC GGAGGCGAAC AATGCCCAAT ACAATCCGAT 351 GAAGACGGGG ATTTGCAACA GCATAGGCAG GCAGCCGCCC AGCGGGTTGA 401 TTTTCTCGTC TGTGTAAAGC TGCATCATCG CCTGTTGTTG CGCCATACGG 451 TCGTCGCCGT ATTTCTCTTT GATGGCTTGC AGTTTGGGTG CGGCGGCACG 501 CATTTTCGCC ATAGAGCGGT AAGAGGCGTT GGTCAATGGA TACAGTACGG 551 CTTTGACGAT GATGGTTAAA ACGATAATCG CCCAGCCCCA GTTGCCGATG 601 ATGTTGTGCA GTTGGTTCAG GAGCCAGAAG AGCGGGGAGG CGAACCAGTG 651 TACTTTGCCG TAGTCTTTGG CCAGTTGCAG GTTGTCGGCG ATGTTTGCGA 701 TGACGGATGT GGTCTGCGGG CCGGCGTAGA GGTTGATGGA GGCTTCGgTT 751 TCGCGCCGTT TTGGATGGCG GCTAAAGGCA CGCTGACGCT GGTGCTGTAC 801 AGCTTGTCGT TGCGGCGTTT GATGTCGATG TTGCACTCGC CTGCGGCGCA 851 AACGCTTTGT CTGCCTTTAG GTTGGAGAAT CCAGGTGGAC ATGAAGTGGT 901 GTTCAATCAT GCCGAGCCAG CCGGTCGGGG TTTTGCGGAT GTATTCGGCC 951 TCGGATTTGC CGGATTTGGC ATCGTCGTCC AAGTCGGAAA AGCTGACTTT 1001 TTGGAAGTTG CCTTCAGGGG TATAA This corresponds to the amino acid sequence <SEQ ID 724; ORF 209>: m209. pep 1 MLRHLGNDFA LGALFFDAAV DVPLLGDGQE VVDYPVQYQT GREEEEHDGE 51 NQRHDFHHFR LHRVGRRRVQ ISLGEHRCRH NDGQDWGVG AAEVGNPTQP 101 RCLAQFYGGE QCPIQSDEDG DLQQHRQAAA QRVDFLVCVK LHHRLLLRHT 151 WAVFLFDGL QFGCGGTHFR HRAVRGVGQW IQYGFDDDG* NDNRPAPVAD 201 DWQLVQEPE ERGGEPVYFA VVFGQLQWG DVCDDGCGLR AGVEVDGGFG 251 FAPFWMAAKG TLTLVLYSLS LRRLMSMLHS PAAQTLCLPL GWRIQVDMKW 301 CSIMPSQPVG VLRMYSASDL PDLASSSKSE KLTFWKLPSG V* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 209 shows 88.5% identity over a 253 aa overlap with a predicted ORF (ORF 209.ng) from N gonorrhoeae: m209/g209 10 20 30 40 50 60 m209. pep MLRHLGΝDFALGALFFDAAVDVPLLGDGQE DYPVQYQTGREEEEHDGEΝQRHDFHHFR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I : I I I I I I I I I II I I I I I I I I I I I g209 MLRHLGΝDFALGALFFDAAVDVPLLGDGQE DHPVEΝQTGREEEEHDGEΝQRHDFHHFR 10 20 30 40 50 60 70 80 90 100 110 120 m209. pep LHRVGRRRVQISLGEHRCRHΝDGQDWGVGAAEVGΝPTQPRCLAQFYGGEQCPIQSDEDG I I I I I II I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I : I : I I I I g209 LHRVGRRRVQIGLGEHRCRHΝDGQDWGVGAAEVGΝPAQPRCLAQFYGGEQCPVQADEDG 70 80 90 100 110 120 130 140 150 160 170 180 m209 . pep DLQQHRQAAAQRVDFLVCVKLHHRLLLRHTVVAVFLFDGLQFGCGGTHFRHRAVRGVGQW I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I : I I I I I I g209 DLQQHRQTAAQRVDFLVFEKLHHRLLLRHTWAVFFFDGLQFGCGGTHFRHRTVGGVGQW 130 140 150 160 170 180 190 200 210 220 230 240 m209.pep IQYGFDDDGXΝDΝRPAPVADDWQLVQEPEERGGEPVYFA FGQLQWGDVCDDGCGLR lllllllll M: I I I I II : II I I I II I I I I I I I I I: I I I I I I I I I I I I I : I I I I I g209 IQYGFDDDGQΝDDCPAPVADΝWQLVQEPEERRCEPVYFTVVFCQLQWGDVCDΝGCGLR 190 200 210 220 230 240 250 260 270 280 290 299 m209.pep AGVEVDGGFGF-APFWMAAKGTLTLVLYSLSLRRLMSMLHSPAAQTLCLPLGWRIQVDMK : I : : I I I I I I g209 TGIQVDRHFRFWPPGWDSG 250 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 725>: a209.seq 1 ATGCTGCGGC ATTTAGGAAA CGACTTCGCC TTGGGCGCGT TGTTTTTCGA 51 TGCTGCGGTT GATGTGCCAT TGCTGGGCGA TGGTCAGGAG GTTGTTGATC 101 ACCCAGTACA ATACCAGACC GGCAGGGAAG AAGAAGAACA TGACGGAGAA 151 AACCAAAGGC ATGATTTTCA TCATTTTCGC CTGCATCGGG TCGGTCGGCG 201 GCGGGTTCAG ATAGGTTTGG GCGAACATCG TTGCCGCCAT AATGATGGGC 251 AGGATGTAGT AGGGGTCGGC GCGGCTGAGG TCGGTAATCC AACCCAGCCA 301 AGGTGCCTGG CGCAATTCTA CGGAGGCGAA CAATGCCCAA TACAATCCGA 351 TGAAGACGGG GATTTGCAAC AGCATAGGCA GGCAGCCGCC CAGCGGGTTG 401 ATTTTCTCGT CTGTGTAAAG CTGCATCATG GCTTGTTGCT GCGCCATACG 451 GTCGTCGCCG TATTTCTCTT TGATGGCTTG CAGTTTGGGC GCGGCGGCAC 501 GCATTTTCGC CATCGAACGG TAAGAGGCGT TGGTCAATGG ATACAGTACG 551 GCTTTGACGA TGATGGTTAA AACGATAATC GCCCAGCCCC AGTTGCCGAT 601 GATGTTGTGC AGTTGGTTCA AAAGCCAAAA GAGGGGGGAG GCGAACCAGT 651 GTACTTTGCC GTAGTCTTTG GCCAGTTGCA GGTTGTCGGC GATGTTTGCG 701 ATAACGGATG TGGTCTGTGG GCCGGCGTAG AGGTTGATGG AGGCTTCGGT 751 TTCGCACCGT TTTGGATAGC GGCTAAAGGC ACGCTGACGC TGGTGCTGTA 801 CAGCTTGTCG TTGCGGCGTT TGATGTCGAT ACGGCAGTCG CCAGCGGCGC 851 AAACGCTTTG TCCGCCTTTG GGTTGGAGGA TCCAGGTGGA CATGAAGTGG 901 TGTTCAATCA TGCCGAGCCA GCCGGTCGGG GTTTTGCGGA TGTATTCGGC 951 CTCGGATTTG CCGGATTTGG CATCGTCGTC CAAGTCGGAG AAGCTGACTT 1001 TTTGGAAGTT GCCTTCAGGG GTATAA This corresponds to the amino acid sequence <SEQ ID 726; ORF 209. a>: a209.pep 1 MLRHLGNDFA LGALFFDAAV DVPLLGDGQE VVDHPVQYQT GREEEEHDGE 51 NQRHDFHHFR LHRVGRRRVQ IGLGEHRCRH NDGQDWGVG AAEVGNPTQP 101 RCLAQFYGGE QCPIQSDEDG DLQQHRQAAA QRVDFLVCVK LHHGLLLRHT 151 WAVFLFDGL QFGRGGTHFR HRTVRGVGQW IQYGFDDDG* NDNRPAPVAD 201 DWQLVQKPK EGGGEPVYFA VVFGQLQWG DVCDNGCGLW AGVEVDGGFG 251 FAPFWIAAKG TLTLVLYSLS LRRLMSIRQS PAAQTLCPPL GWRIQVDMKW 301 CSIMPSQPVG VLRMYSASDL PDLASSSKSE KLTFWKLPSG V* m209/a209 95.6% identity in 341 aa overlap 10 20 30 40 50 60 m209. pep MLRHLGNDFALGALFFDAAVDVPLLGDGQEWDYPVQYQTGREEEEHDGENQRHDFHHFR I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I : I I I II I I I I I I I I I I I I I I I I I I I I I a209 MLRHLGNDFALGALFFDAAVDVPLLGDGQEWDHPVQYQTGREEEEHDGENQRHDFHHFR 10 20 30 40 50 60 70 80 90 100 110 120 m209.pep LHRVGRRRVQISLGEHRCRHNDGQDVVGVGAAEVGNPTQPRCLAQFYGGEQCPIQSDEDG I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I II II I I I I I I I I I I I I I I I I I I I I I a209 LHRVGRRRVQIGLGEHRCRHNDGQDWGVGAAEVGNPTQPRCLAQFYGGEQCPIQSDEDG 70 80 90 100 110 120 130 140 150 160 170 180 m209. pep DLQQHRQAAAQRVDFLVCVKLHHRLLLRHTWAVFLFDGLQFGCGGTHFRHRAVRGVGQW I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I : I I I I I I I a209 DLQQHRQAAAQRVDFLVCVKLHHGLLLRHT WAVFLFDGLQFGRGGTHFRHRTVRGVGQW 130 140 150 160 170 180 190 200 210 220 230 240 m209 . pep IQYGFDDDGXNDNRPAPVADDWQLVQEPEERGGEPVYFAWFGQLQWGDVCDDGCGLR I I I I I I I I I I I I I I I I I I I I I I I I I I I : I : I I I I I I I I I I I I I I I I I I I I I I I : I I I I a209 IQYGFDDDGXNDNRPAPVADDWQLVQKPKEGGGEPVYFAWFGQLQVVGDVCDNGCGLW 190 200 210 220 230 240 250 260 270 280 290 300 m209 . pep AGVEVDGGFGFAPFWMAAKGTLTLVLYSLSLRRLMSMLHSPAAQTLCLPLGWRIQVDMKW I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I : : I I I I I I I I I I I I I I I I I I I I a209 AGVEVDGGFGFAPFWIAAKGTLTLVLYSLSLRRLMSIRQSPAAQTLCPPLGWRIQVDMKW 250 260 270 280 290 300 310 320 330 340 m209.pep CSIMPSQPVGVLRMYSASDLPDLASSSKSEKLTFWKLPSGVX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a209 CSIMPSQPVGVLRMYSASDLPDLASSSKSEKLTFWKLPSGVX 310 320 330 340 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 727>: g211. seq 1 atgttgcgga ttgctgctgc caatcagttg ggcggtcgaa atggtgcggc 51 ggtgggaaac ggggtcgata agtttgggcg tggtgctgat aatcaggttg 101 agtttttgga aggaaacctg attgtagtcg gcgcgtccgg gcgtgccgct 151 gtaacggtag ccgtggcgca attcgagcgt gcgtttgttg tccttcagcg 201 agaagttacc ttctttggcg aagatgatgt tgtcgccgcc gtttttgtcc 251 tgttcgcgca ggaacaggtt tttcatgatg ccggattcgg tgtcaaaggt 301 ttcgacgaaa taaaccctgc cgttgcgctt gcccaagtta ttgaactcgc 351 cggcttccac caaagacaat tcctgcttct gcttcaaaat ttcggcatat 401 tcgcggctgc gcagctctgc ccacggtatc acccaaagct gcatgacggc 451 aatcaggatg gcaaacggca cggcaaactg catgacgggg cgtatccact 501 gtttcaacgc caatccgcag gatag This corresponds to the amino acid sequence <SEQ ID 728; ORF 21 l.ng>: g211.pep 1 MLRIAAAΝQL GGRΝGAAVGΝ GVDKFGRGAD ΝQVEFLEGΝL IWGASGRAA 51 VTVAVAQFER AFWLQREVT FFGEDDWAA VFVLFAQEQV FHDAGFGVKG 101 FDEIΝPAVAL AQVIELAGFH QRQFLLLLQΝ FGIFAAAQLC PRYHPKLHDG 151 ΝQDGKRHGKL HDGAYPLFQR QSAG* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 729>: m211.seq 1 ATGTTGCGGG TTGCTGCTGC CAATCAGTTG GGCGGTCGGA ATGGTACGGC 51 GGTGGGAAAC GGGGTCGATG AGTTTGGGCG TGGTGCTGAT AATCAGGTTG 101 AGTTTTTGGA AGGAAACCTG ATTGTAGTCG GCGCGTCCGG GCGTGCCGCT 151 GTAACGGTAG CCGTGGCGCA ATTCGAGCGT GCGTTTGTTG TCGTTCAGCG 201 AGAAGTTACC TTCTTTGGCG AAGATGATGT TGTCGCCGCC GTTTTTGTCC 251 TGTTCGCGCA GGAACAGGTT TTTCATGATG CCGGATTCGG TATCGAAGGT 301 TTCGACAAAA TAAACCCTGC CGTTGCGCTT GCCCAAACTG TTGAACTCGC 351 CTGCCTCCAC CAAAGACAAT TCCTGCTTCT GCTTCAGGAT TTCAGCGTAT 401 TCGCGGCTGC GTAGCTCTGC CCACGGTATC ACCCAAAGCT GCATGACGGC 451 AACCAAAACG GCAAACGGCA CGGCAAACTG CATCACCGGG CGTATCCATT 501 GTTTCAATGC CAATCCGCAg GATAG This corresponds to the amino acid sequence <SEQ ID 730; ORF 211>: m211.pep 1 MLRVAAAΝQL GGRΝGTAVGΝ GVDEFGRGAD ΝQVEFLEGΝL IWGASGRAA 51 VTVAVAQFER AFVWQREVT FFGEDDWAA VFVLFAQEQV FHDAGFGIEG 101 FDKIΝPAVAL AQTVELACLH QRQFLLLLQD FSVFAAAXLC PRYHPKLHDG 151 ΝQΝGKRHGKL HHRAYPLFQC QSAG* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 211 shows 89.1% identity over a 1 '4 aa overlap with a predicted ORF (ORF 21 l.ng) from N. gonorrhoeae: m21l/g211 10 20 30 40 50 60 m211.pep MLRVAAAΝQLGGRΝGTAVGΝGVDEFGRGADΝQVEFLEGΝLIWGASGRAAVTVAVAQFER g211 MLRIAAAΝQLGGRΝGAAVGΝGVDKFGRGADΝQVEFLEGΝLIWGASGRAAVTVAVAQFER 10 20 30 40 50 60 70 80 90 100 110 120 m211. pep AFVWQREVTFFGEDDWAAVFVLFAQEQVFHDAGFGIEGFDKIΝPAVALAQTVELACLH g211 AFWLQREVTFFGEDDWAAVFVLFAQEQVFHDAGFGVKGFDEINPAVALAQVIELAGFH 70 80 90 100 110 120 130 140 150 160 170 m211 . pep QRQFLLLLQDFSVFAAAXLCPRYHPKLHDGNQNGKRHGKLHHRAYPLFQCQSAGX M M I M I h h M I M l l l l l l l l l l l l l h l l l l l l l l l l l l l l M M g211 QRQFLLLLQNFGIFAAAQLCPRYHPKLHDGNQDGKRHGKLHDGAYPLFQRQSAG 130 140 150 160 170 The following partial DNA sequence was identified in N meningitidis <SEQ ID 731>: a211. seq 1 ATGTTGCGGG TTGCTGCTGC CAATCAGTTG GGCGGTCGGA ATGGTACGGC 51 GGTGGGAAAC GGGGTCGATG AGTTTGGGCG TGGTGCTGAT AATCAGGTTG 101 AGTTTTTGGA AGGAAACCTG ATTGTAGTCG GCGCGTCCGG GCGTGCCGCT 151 GTAACGGTAG CCGTGGCGCA ATTCGAGCGT GCGTTTGTTG TCGTTCAGCG 201 AGAAGTTACT TTCTTTGGCG AAGATGATGT TGTCGCCGCC GTTTTTGTCC 251 TGTTCGCGCA GGAACAGGTT TTTCATGATG CCGGATTCGG TATCGAAGGT 301 TTCGACAAAA TAAACCCTGC CGTTGCGCTT GCCCAAACTG TTGAACCCGC 351 CTGCCTCCAC CAAAGACAAT TCCTGCTTCT GCTTCAGGAT TTCAGCGTAT 401 TCGCGGCTGC GTAGCTCTGC CCACGGTATC ACCCAAAGCT GCATGACGGC 451 AACCAAAACG GCAAACGGCA CGGCAAACTG CATCACCGGG CGTATCCATT 501 GTTTCAATGC CAATCCGCAG GATAG This corresponds to the amino acid sequence <SEQ ID 732; ORF 21 l.a>: a211.pep 1 MLRVAAAΝQL GGRΝGTAVGΝ GVDEFGRGAD ΝQVEFLEGΝL IWGASGRAA 51 VTVAVAQFER AFVWQREVT FFGEDDWAA VFVLFAQEQV FHDAGFGIEG 101 FDKIΝPAVAL AQTVEPACLH QRQFLLLLQD FSVFAAA*LC PRYHPKLHDG 151 ΝQΝGKRHGKL HHRAYPLFQC QSAG* m21 l/a211 99.4% identity in 174 aa overlap 10 20 30 40 50 60 m211 . pep MLRVAAAΝQLGGRΝGTAVGΝGVDEFGRGADΝQVEFLEGΝLI WGASGRAAVTVAVAQFER I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a211 MLRVAAAΝQLGGRΝGTAVGΝGVDEFGRGADΝQVEFLEGΝLIWGASGRAAVTVAVAQFER 10 20 30 40 50 60 70 80 90 100 110 120 m211. pep AFVWQREVTFFGEDDWAAVFVLFAQEQVFHDAGFGIEGFDKIΝPAVALAQTVELACLH I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a211 AFVWQREVTFFGEDDWAAVFVLFAQEQVFHDAGFGIEGFDKIΝPAVALAQTVEPACLH 70 80 90 100 110 120 130 140 150 160 170 m211 . pep QRQFLLLLQDFSVFAAAXLCPRYHPKLHDGΝQΝGKRHGKLHHRAYPLFQCQSAGX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a211 QRQFLLLLQDFSVFAAAXLCPRYHPKLHDGΝQΝGKRHGKLHHRAYPLFQCQSAGX 130 140 150 160 170 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 733>: g212.seq (partial) 1 atggacaatc tcgtatggga cggcattccc gacatccgca cactcgacca 51 aaccatccgc aaacacgcac acccgctcaa cctgattgtc tgcctccccg 101 ataatcagat tcccgatttt caaaccgcac aagatgcttc ggactcggaa 151 tgccgtctga agcaccgttt ggatcaggca acccagtgcc tccagttcga 201 cagcatcaac ctcatcgaac acatcctgcc cgatgtccgc ttctggctgg 251 ttcccccttc acgcacccgc cgcctgcacg aacacttcca ccacatttcc 301 tggcagaccg aagccatccc gcaaaccgaa agcaagtccg acaaaccctg 351 gtttgcactt ccacaaacat ccgaacggaa aaaaccggaa cacgtcctcg 401 tcatcggtgc aggcattgcc ggcgcatcga ccgcccacgc cttagcatca 451 cacggcattt ccgttaccgt attggaagcc cgaaaagccg ctcaagccgc 501 cagcggcaac cggcaagggc tgctttacgc caaaatctcg ccgcacgaca 551 ccggacagac cgaactgctg cttgccggct acggctacac caaacgcctg 601 ctcggacaca tcctgcccga ctccgacact tggggcggca acggcatcat 651 ccacctcaat tacagccgca ccgaacaaca acgcaatcac gaattgggtt 701 tgcaaaaaca ccataaccac ctctaccgca gcatcacgtc tgcagaagcc 751 gaaaaaatcg ccggcatccc gctgaacacg ccctacgccg aaccattatg 801 cggactctac tggcaacacg gcgtatggct caatccgccc gcattcgtcc 851 gcaccctcct cagccatccg ctgatcgaac tatatgaaaa cacaacgtta 901 accggcattt cccacgacgg agaaaagtgg attgcaagca cgccaaacgg 951 cacatttacc gccacacaca tcatctactg caccggcgcg cacagcccct 1001 gcctgcccga aaccaacctc gccgccctac ccctcaggca aatacgcgga 1051 caaaccggcc tcacaccgtc caccccgttt tccgaacaac tgcgttgcgc 1101 cgtttcaggc gaaagctaca tcagcccgtc gtggcacgga ctgcactgct 1151 acggcgcgag ttttattccc aacagcagca ataccggatg gaacgaagcc 1201 gaagaagcct caaaccgcca agcattggca caccttaacc ccgcccttgc 1251 cgaatcattg ttt... This corresponds to the amino acid sequence <SEQ ID 734; ORF 212.ng>: g212.pep (partial) 1 MDNLVWDGIP DIRTLDQTIR KHAHPLNLIV CLPDNQIPDF QTAQDASDSE 51 CRLKHRLDQA TQCLQFDSIN LIEHILPDVR FWLVPPSRTR RLHEHFHHIS 101 WQTEAIPQTE SKSDKPWFAL PQTSERKKPE HVLVIGAGIA GASTAHALAS 151 HGISVTVLEA RKAAQAASGN RQGLLYAKIS PHDTGQTELL LAGYGYTKRL 201 LGHILPDSDT WGGNGIIHLN YSRTEQQRNH ELGLQKHHNH LYRSITSAEA 251 EKIAGIPLNT PYAEPLCGLY WQHGVWLNPP AFVRTLLSHP LIELYENTTL 301 TGISHDGEKW IASTPNGTFT ATHIIYCTGA HSPCLPETNL AALPLRQIRG 351 QTGLTPSTPF SEQLRCAVSG ESYISPSWHG LHCYGASFIP NSSNTGWNEA 401 EEASNRQALA HLNPALAESL F... The following partial DNA sequence was identified in N meningitidis <SEQ ID 735>: m212.seq 1 ATGGACAATC TCGTATGGGA CGGCATTCCC GACATCCGCA CACTCGACCA 51 AGCCATCCGC AAACACGCAC CCCCGCTCAA CCTGATTATC TGCCTCCCCG 101 ATAATCAGAT TCCCGATTTT CAAACCGCAC AAGATGCTTC GGACGCGGAA 151 TGCCGTCTGA AGCACCGTTT GGATCAGGCA ATGCAGTGCC TCCAGTTCGA 201 CAGCATCAAC CTCATCGAAC ACATCCTGCC CGATGTCCGC TTCTGGCTGG 251 TTCCCCCTTC ACGCACCCAC CACCTGCACG AACATTTCCA CCACATTTCC 301 TGGCAGACCG AAGCCATCCC GCAAACCGAA AGCAAGCCCG ACAAACCCTG 351 GTTTGCACTT CCACAAACAT CCGAACGGCA AAAACCGGAA CACATCCTCG 401 TTATCGGCGC GGGCATATCC GGCGCGGCAA CCGCCCACGC CTTAGCATCA 451 CACGGCATTT CCGTTACCGT ATTGGAAGCC CGAAAAGCCG CCCAAGCCGC 501 CAGCGGCAAC CGCCAAGGGC TGCTCTACGC CAAAATCTCG CCGCACGACA 551 CCGAACAGAC CGAACTTTTG CTTGCCGGCT ACGGCTACAC CAAACGCCTG 601 CTCGGACACA TCCTGCCCGA ATCCGAAACC TGGGGCGGCA ACGGCATCAT 651 CCACCTCAAT TACAGCCGCA CCGAACAACA ACGCAATCAC GAATTGGGTT 701 TGCAAAAACA CCATAACCAC CTCTACCGCA GCATCACATC TGCAGAAGCC 751 GAAAAAATCG CCGGTATCCC ACTGTCCGTC CCATACGACC ACCCTTCATG 801 CGGACTCTAC TGGCAACACG GCGTATGGCT CAATCCACCC GCATTCGTCC 851 GCACCCTCCT CAACCATCCG CTCATTGGAC TACACGAAGA CACACCCTTG 901 ACCGACATTT CCCACGACGG GGaAAAGTGG ATTGCAAGCA CGCCAAACGG 951 CACATTTACC GCCACACACA TCATCTACTG CACCGGTGCG AACAGCCCCT 1001 ACCTACCCGA AACCAACCTC GCCGCCCTGC CTCTCAGGCA AATACGCGGA 1051 CAAACCGGCC TCACACCGTC CACCCCGTTT TCCGAACAAC TGCGTTGCGC 1101 CGTTTCAGGC GAAAGCTACA TCAGCCCGTC GTGGCACGGA CTGCACTGCT 1151 ACGGCGCGAG TTTTATTCCC AACAGCAGCC ATACCGGATG GAACGAAGCC 1201 GAAGAAGCCT CAAACCGCCA AGCATTGGCA CACCTTAACC CCGCCCTTTC 1251 CGAATCATTG TTTGCCGCCA ACCCAAACCC CCAAAAACAC CAAGGGCACG 1301 CCGCCATACG CTGCGACAGC CCCGACCACC TTCCCCTAGT CGGCGCACTC 1351 GGCGACATTG CCGCCATGCG GCAGACCTAC ACCAAACTCG CGCTGGACAA 1401 AAACTACCGC ATCGACACCC CATGCCCATA CCTGCCTAAT GCCTACGTCA 1451 ACACCGCGCA CGGCACCCGC GGACTCGCCA CCGCCCCCAT CTGCGCCGCC 1501 GmCA TGCAG CCCAAATCsT AGGCyTGCCC CATCCCTTTT yAcAAcGCCT 1551 gCGCCACGCC cTAcACCCCA ACCGCACCAT CATCCGCGCC ATCGTCAGAA 1601 GGAAGGATCT AACCCCTTAA This corresponds to the amino acid sequence <SEQ ED 736; ORF 212>: m212.pep 1 MDNLVWDGIP DIRTLDQAIR KHAPPLNLII CLPDNQIPDF QTAQDASDAE 51 CRLKHRLDQA MQCLQFDSIN LIEHILPDVR FWLVPPSRTH HLHEHFHHIS 101 WQTEAIPQTE SKPDKPWFAL PQTSERQKPE HILVIGAGIS GAATAHALAS 151 HGISVTVLEA RKAAQAASGN RQGLLYAKIS PHDTEQTELL LAGYGYTKRL 201 LGHILPESET WGGNGIIHLN YSRTEQQRNH ELGLQKHHNH LYRSITSAEA 251 EKIAGIPLSV PYDHPSCGLY WQHGVWLNPP AFVRTLLNHP LIGLHEDTPL 301 TDISHDGEKW IASTPNGTFT ATHIIYCTGA NSPYLPETNL AALPLRQIRG 351 QTGLTPSTPF SEQLRCAVSG ESYISPSWHG LHCYGASFIP NSSHTGWNEA 401 EEASNRQALA HLNPALSESL FAANPNPQKH QGHAAIRCDS PDHLPLVGAL 451 GDIAAMRQTY TKLALDKNYR IDTPCPYLPN AYVNTAHGTR GLATAPICAA 501 XXAAQIXGLP HPFXQRLRHA LHPNRTIIRA IVRRKDLTP* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 212 shows 92.9% identity over a 421 aa overlap with a predicted ORF (ORF 212.ng) from N gonorrhoeae: m212/g212 10 20 30 40 50 60 m212.pep MDΝLVWDGIPDIRTLDQAIRKHAPPLΝLIICLPDΝQIPDFQTAQDASDAECRLKHRLDQA g212 MDΝLVWDGIPDIRTLDQTIRKHAHPLΝLIVCLPDΝQIPDFQTAQDASDSECRLKHRLDQA 10 20 30 40 50 60 70 80 90 100 110 120 m212.pep MQCLQFDSIΝLIEHILPDVRFWLVPPSRTHHLHEHFHHISWQTEAIPQTESKPDKPWFAL 111111111111111111111111 j 111 :H 111111111111 M 111111 I [ 11111 g212 TQCLQFDSIΝLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQTEAIPQTESKSDKPWFAL 70 80 90 100 110 120 130 140 150 160 170 180 m21 .pep PQTSERQKPEHILVIGAGISGAATAHALASHGISVTVLEARKAAQAASGΝRQGLLYAKIS M 11 M : 1111 = 11111 i I h 11111111111111111 ! 111111 ! 11111111 ! 111 g212 PQTSERKKPEHVLVIGAGIAGASTAHALASHGISVTVLEARKAAQAASGΝRQGLLYAKIS 130 140 150 160 170 180 190 200 210 220 230 240 m212.pep PHDTEQTELLLAGYGYTKRLLGHILPESETWGGΝGIIHLΝYSRTEQQRΝHELGLQKHHΝH MM MMMIMMMMMMIhhMMMMMMMMMMMMIMMM g212 PHDTGQTELLLAGYGYTKRLLGHILPDSDTWGGΝGIIHLΝYSRTEQQRΝHELGLQKHHΝH 190 200 210 220 230 240 250 260 270 280 290 300 m212.pep LYRSITSAEAEKIAGIPLSVPYDHPSCGLYWQHGVWLΝPPAFVRTLLΝHPLIGLHEDTPL M M M M M M M M I h M I M 1 1 1 1 1 1 I I I I 1 1 1 I I 1 1 1 1 1 1 = I I I I h h l I g212 LYRSITSAEAEKIAGIPLΝTPYAEPLCGLYWQHGVWLΝPPAFVRTLLSHPLIELYEΝTTL 250 260 270 280 290 300 310 320 330 340 350 360 m212. pep TDISHDGEKWIASTPΝGTFTATHIIYCTGAΝSPYLPETΝLAALPLRQIRGQTGLTPSTPF I g212 TGISHDGEKWIASTPΝGTFTATHIIYCTGAHSPCLPETΝLAALPLRQIRGQTGLTPSTPF 310 320 330 340 350 360 370 380 390 400 410 420 m21 .pep SEQLRCAVSGESYISPSWHGLHCYGASFIPΝSSHTGWΝEAEEASΝRQALAHLΝPALSESL g212 SEQLRCAVSGESYISPSWHGLHCYGASFIPNSSNTGWNEAEEASNRQALAHLNPALAESL 370 380 390 400 410 420 430 440 450 460 470 480 m212. pep FAANPNPQKHQGHAAIRCDSPDHLPLVGALGDIAAMRQTYTKLALDKNYRIDTPCPYLPN I g212 F The following partial DNA sequence was identified in N meningitidis <SEQ ID 737>: a212. seq 1 ATGGACAATC TCGCATGGAA CGGCATTCCC GACATCCGCA CACTCGACCA 51 AACCATCCGC AAACACGCAC ACCCGCTCAA CCTGATTGTC TGCCTCCCCG 101 ATAATCAGAT TCCCAATTTT CAAACCGCAC AAGATGCTTC GGACGCGGAA 151 TGCCGTCTGA AGCACCGTTT GGATCAGGCA ACCCAGTGCC TCCAGTTCGA 201 CAGCATCAAC CTGATTGAAC ACATCCTGCC CGATGTCCGC TTCTGGCTGG 251 TTCCCCCTTC ACGCACCCGC CGCCTGCACG AACACTTCCA CCACATTTCC 301 TGGCAGACCG AAGCCATCCC GCAAACCGAA AGTAAGCCCG ACAAACCCTG 351 GTTTGCACTT CCACAAACAT CCGAACGGCA AAAACCGGAA CACATCCTCG 401 TTATCGGAGC GGGCATATCC GGCGCGGCAA CCGCCCACGC CTTAGCATCA 451 TACGGCATTT CCGTTACCGT ATTGGAAGCC CGAAAAGCCG CCCAAGCCGC 501 CAGCGGCAAC CGCCAAGGGC TGCTCTACGC CAAAATCTCG CCGCACGACA 551 CCGAACAAAC CGAACTGCTG CTTGCCGGCT ACGGCTACAC CAAACGCCTG 601 CTCGGACATA TCCTGCCCGA ATCCGAAACC TGGGGCGGCA ACGGCATCAT 651 CCACCTCAAT TACAGCCGCA CCGAACAACA ACGCAATCAC GAATTGGGTT 701 TGCAAAAACA CCATAACCAC CTCTACCGCA GCATCACGCA GGCAGAAGCC 751 GAAAAAATCG CCGGCATCCC TCTGAACACG CCCTACGCCG AACCATTATG 801 CGGACTGTTT TGGCAGTACG GCGTATGGCT CAATCCTCCC ACATTCGTCC 851 GCGCCCTCCT CAGCCATCCG CTCATTGGAC TACACGAAGA CACACCGTTA 901 ACCGACATTT CCCACGACGG GGAAAAGTGG ATTGCAAGCA CGCCAAACGG 951 CACATTTACC GCCACACACA TCATCTACTG CACCGGTGCG AACAGCCCCT 1001 ACCTACCCGA AACCAACCTC GCCACCCTGC CCCTCAGGCA AATACGCGGA 1051 CAAACCGGCC TCACACCGTC CACCCCGTTT TCCGAACAAC TGCGTTGCGC 1101 CGTTTCAGGC GAAAGCTACA TCAGCCCGTC GTGGCACGGA CTGCACTGCT 1151 ACGGCGCGAG TTTTATTCCC AACAGCAGCC ATACCGGATG GAACGAAGCC 1201 GAAGAAGCCT CAAACCGCCA AGCATTGGCA CACCTTAACC CCGCCCTTTC 1251 CGAATCATTG TTTGCCGCCA ACCCAAACCC CCAAAAACAC CAAGGGCACG 1301 CCGCCATACG CTGCGACAGC CCCGACCACC TTCCCCTAGT CGGCGCACTC 1351 GGCGACATTG CCGCTATGCA ACAAACTTAC GCCAAACTCG CGCTGGACAA 1401 AAACTATCGC ATCGATGCCC CCTGCCCGTA CCTGCCCAAT GCCTACGCCA 1451 ACACCGCCCA CGGCACACGC GGGCTTGCCA CCGCCCCCAT CTGCGCCGCC 1501 GCCGTTGCAG CCGAAATCCT AGGCTTGCCC CATCCCCTCT CAAAACGCCT 1551 GCGCCACGCC CTACACCCCA ACCGCGCCAT CATCCGCGCC ATCGTCAGAA 1601 GGAAGGATCT AACCCCTTAA This corresponds to the amino acid sequence <SEQ ID 738; ORF 212. a>: a212.pep 1 MDΝLAWΝGIP DIRTLDQTIR KHAHPLNLIV CLPDNQIPNF QTAQDASDAE 51 CRLKHRLDQA TQCLQFDSIN LIEHILPDVR FWLVPPSRTR RLHEHFHHIS 101 WQTEAIPQTE SKPDKPWFAL PQTSERQKPE HILVIGAGIS GAATAHALAS 151 YGISVTVLEA RKAAQAASGN RQGLLYAKIS PHDTEQTELL LAGYGYTKRL 201 LGHILPESET WGGNGIIHLN YSRTEQQRNH ELGLQKHHNH LYRSITQAEA 251 EKIAGIPLNT PYAEPLCGLF WQYGVWLNPP TFVRALLSHP LIGLHEDTPL 301 TDISHDGEKW IASTPNGTFT ATHIIYCTGA NSPYLPETNL ATLPLRQIRG 351 QTGLTPSTPF SEQLRCAVSG ESYISPSWHG LHCYGASFIP NSSHTGWNEA 401 EEASNRQALA HLNPALSESL FAANPNPQKH QGHAAIRCDS PDHLPLVGAL 451 GDIAAMQQTY AKLALDKNYR IDAPCPYLPN AYANTAHGTR GLATAPICAA 501 AVAAEILGLP HPLSKRLRHA LHPNRAIIRA IVRRKDLTP* m212/a212 93.7% identity in 539 aa overlap 10 20 30 40 50 60 m212.pep MDNLVWDGIPDIRTLDQAIRKHAPPLNLIICLPDNQIPDFQTAQDAΞDAECRLKHRLDQA I I I I : I : I M I I II I I I : I I I I I I I I I : I I II I I I I : I I I I I I I I I I I I I I I II I I I I a212 MDNLAWNGIPDIRTLDQTIRKHAHPLNLIVCLPDNQIPNFQTAQDASDAECRLKHRLDQA 10 20 30 40 50 60 70 80 90 100 110 120 m212. ■Pep MQCLQFDSINLIEHILPDVRFWLVPPSRTHHLHEHFHHISWQTEAIPQTESKPDKPWFAL I I I I I I I I I I I I I I I I I I I I I I I I I I I I::| I I I I I I I I I I I I I I I I I I I I I I I I I I I I a212 TQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQTEAIPQTESKPDKPWFAL 70 80 90 100 110 120 130 140 150 160 170 180 m212. .pep PQTSERQKPEHILVIGAGISGAATAHALASHGISVTVLEARKAAQAASGNRQGLLYAKIS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I :| I I I I I I I I I I I I I I I I I I I I I I I I I I I I a212 PQTSERQKPEHILVIGAGISGAATAHALASYGISVTVLEARKAAQAASGNRQGLLYAKIΞ 130 140 150 160 170 180 190 200 210 220 230 240 m212 .pep PHDTEQTELLLAGYGYTKRLLGHILPESETWGGNGIIHLNYSRTEQQRNHELGLQKHHNH I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a212 PHDTEQTELLLAGYGYTKRLLGHILPESETWGGNGIIHLNYSRTEQQRNHELGLQKHHNH 190 200 210 220 230 240 250 260 270 280 290 300 m212.pep LYRSITSAEAEKIAGIPLSVPYDHPSCGLYWQHGVWLNPPAFVRTLLNHPLIGLHEDTPL I I I I I I :| I I I I I I I I I I :: I I : I I I I : I I : I I I I I I I : I I I : I I : I I I I I I I I I I I I a212 LYRΞITQAEAEKIAGIPLNTPYAEPLCGLFWQYGVWLNPPTFVRALLSHPLIGLHEDTPL 250 260 270 280 290 300 310 320 330 340 350 360 m212. .pep TDIΞHDGEKWIASTPNGTFTATHIIYCTGANSPYLPETNLAALPLRQIRGQTGLTPSTPF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I :| I I I I I I I I I I I I I I I I I a212 TDISHDGEKWIASTPNGTFTATHIIYCTGANΞPYLPETNLATLPLRQIRGQTGLTPΞTPF 310 320 330 340 350 360 370 380 390 400 410 420 m212. .pep SEQLRCAVSGESYISPSWHGLHCYGAΞFIPNSSHTGWNEAEEASNRQALAHLNPALSESL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a212 SEQLRCAVSGESYISPΞWHGLHCYGASFIPNSSHTGWNEAEEASNRQALAHLNPALΞEΞL 370 380 390 400 410 420 430 440 450 460 470 480 m212, .pep FAANPNPQKHQGHAAIRCDSPDHLPLVGALGDIAAMRQTYTKLALDKNYRIDTPCPYLPN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I :| I I: I I I I I I I I I I I : I I I I I I I a212 FAANPNPQKHQGHAAIRCDSPDHLPLVGALGDIAAMQQTYAKLALDKNYRIDAPCPYLPN 430 440 450 460 470 480 490 500 510 520 530 540 m212, .pep AYVNTAHGTRGLATAPICAAXXAAQIXGLPHPFXQRLRHALHPNRTIIRAIVRRKDLTPX I I : I I I I I I I I I I I I I I I I I I I : I Mill: : I I I I I I I I I I : I I I I I I I I I I I II I a212 AYANTAHGTRGLATAPICAAAVAAEILGLPHPLSKRLRHALHPNRAIIRAIVRRKDLTPX 490 500 510 520 530 540 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 739>: g214.seq 1 atgatacaaa agatatgtaa gctatttgtt ttaattgtaa tttttgcaac 51 ttctcccgct tttgcccttc aaagcgacag cagacggccc atccaaatcg 101 aagccgacca aggttcgctc gatcaagcca accaaaggac cacatttagc 151 ggcaatgtca tcatcagaca gggtacgctc aacatttccg cctcgtgtgt 201 caacgtcaca cgcggcaggc aaaggcggcg aatccgtgag ggcggaaggt 251 tcgcccgtcc gcttcagcca aacgttggac gggggcaaag ggacggtgcg 301 cggtcaggca aacaacgtta cctattcctc cgcaggaagc actgtcgttc 351 tgaccggcaa tgccaaagtg cagcgcggcg gcgacgttgc cgaaggtgcg 401 gtcattacct acaacaccaa aaccgaagtc tataccatca acggcagcac 451 gaaatcgggt gcgaaatccg cttccaaaac cggcagggtc agcgtcgtca 501 tccagccttc aagcacacaa aaaaccgaat aaccccgatg ccgtctgaaa 551 cggaaacgca gttcagacgg catttgccga ccgaaatgcc gagaagagat 601 tattga This corresponds to the amino acid sequence <SEQ ID 740; ORF 214.ng>: g214 . pep 1 MIQKICKLFV LIVIFATSPA FALQSDSRRP IQIEADQGSL DQANQRTTFS 51 GNVIIRQGTL NISASCVNVT RGRQRRRIRE GGRFARPLQP NVGRGQRDGA 101 RSGKQRYLFL RRKHCRSDRQ CQSAARRRRC RRCGHYLQHQ NRSLYHQRQH 151 EIGCEIRFQN RQGQRRHPAF KHTKNRITPM PSETETQFRR HLPTEMPRRD 201 Y The following partial DNA sequence was identified in N meningitidis <SEQ ID 741>: m214.seq (partial) 1 ATGATACAAA AGATATGTAA GCTATTTGTT TTAATAGCAT TTTTTTCGGC 51 GTCCCCCGCT TTTGCCCTTC AAAGCGACAG CAGGCAGCCT ATTCAGATTG 101 AGGCCGACCA AGGTTCGCTC GATCAAGCCA ACCAAAGCAC CACATTCAGC 151 GGAAACGTCG TCATCAGACA GGGTACGCTC AATATTTCCG CCGCCCGCGT 201 CAATGTTACA CGCGGCGGCA AAGGCGGCGA ATCCGTGAGG GCGGAAGGTT 251 CGCCAGTCCG CTTCAGCCAG ACATTGGACG GCGGCAAAGG CACGGTGCGC 301 GGACAGGCAA ACAACGTTGC TTATTCATCT GCAGGCAGCA CCGTAGTCTT 351 AACCGGTAAT GCCAAAGTAC AGCGCGGCGG CGATGTCGCC GAAGGTGCGG 401 TGATTACATA CAACACCAAA ACCGAAGTCT ATACCATCAG CGGCAGCACA 451 AAATT... This corresponds to the amino acid sequence <SEQ ID 742; ORF 214>: m214.pep (partial) 1 MIQKICKLFV LIAFFSASPA FALQSDSRQP IQIEADQGSL DQAΝQSTTFS 51 GΝWIRQGTL ΝISAARVΝVT RGRQRRRIRE GGRFASPLQP DIGRRQRHGA 101 RTGKQRCLFI CRQHRSLΝR* CQSTARRRCR RRCGDYIQHQ NRSLYHQRQH 151 KI... Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 214 shows 80.3% identity over a 152 aa overlap with a predicted ORF (ORF 214.ng) from N gonorrhoeae: m214/g214 10 20 30 40 50 60 m214.pep MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLDQAΝQSTTFSGΝWIRQGTL M M M M M I h M M M I M M M h M M M M M M M M g214 MIQKICKLFVLIVIFATSPAFALQSDSRRPIQIEADQGSLDQAΝQRTTFSGΝVI IRQGTL 10 20 30 40 50 60 70 80 90 100 110 120 m214.pep ΝISAARVΝVTRGRQRRRIREGGRFASPLQPDIGRRQRHGARTGKQRCLFICRQHRSLΝRX 1 1 1 1 = M l h M I I I l l h l l l l h i M g214 ΝISASCVΝVTRGRQRRRIREGGRFARPLQPΝVGRGQRDGARSGKQRYLFLRRKHCRSDRQ 70 80 90 100 110 120 130 140 150 m214.pep CQSTARRRCRRRCGDYIQHQΝRSLYHQRQHKI l l h l l l l M M g214 CQSAARRRRCRRCGHYLQHQΝRSLYHQRQHEIGCEIRFQΝRQGQRRHPAFKHTKΝRITPM 130 140 150 160 170 180 g214 PSETETQFRRHLPTEMPRRDY 190 200 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 743>: a214. seq 1 ATGATACAAA AGATATGTAA GCTATTTGTT TTAATAGCAT TTTTTTCGGC 51 GTCCCCCGCT TTTGCCCTTC AAAGCGACAG CAGGCAGCCT ATTCAGATTG 101 AGGCCGACCA AGGTTCGCTC GATCAAGCCA ACCAAAGCAC CACATTCAGC 151 GGAAACGTCG TCATCAGACA GGGTACGCTC AATATTTCCG CCGCCCGCGT 201 CAATGTTACA CGCGGC . GGC AAAGGCGGCG AATCCGTGAG GGCGGAAGGT 251 TCGCCAGTCC GCTTCAGCCA GACATTGGAC GGCGGCAAAG GCACGGTGCG 301 CGGACAGGCA AACAACGTTG CTTATTCATC TGCAGGCAGC ACCGTAGTCT 351 TAACCGGTAA TGCCAAAGTA CAGCGCGGCG GCGATGTCGC CGAAGGTGCG 401 GTGATTACAT ACAACACCAA AACCGAAGTC TATACCATCA GCGGCAGCAC 451 AAAATCCGGC GCAAAATCCG CTTCCAAATC CGGCAGGGTC AGCGTCGTTA 501 TCCAGCCTTC GAGTACGCAA AAATCCGAAT AATCCCAATG CCGTCTGAAA 551 CATAAACCTG GTTCGGACGG CATTTGCCGA CCGAAATATT GAAGAGATAT 601 TTATGA This corresponds to the amino acid sequence <SEQ ID 744; ORF 214.a>: a214.pep 1 MIQKICKLFV LIAFFSASPA FALQSDSRQP IQIEADQGSL DQANQSTTFS 51 GNWIRQGTL NISAARVNVT RGXQRRRIRE GGRFASPLQP DIGRRQRHGA 101 RTGKQRCLFI CRQHRSLNR* CQSTARRRCR RRCGDYIQHQ NRSLYHQRQH 151 KIRRKIRFQI RQGQRRYPAF EYAKIRIIPM PSET*TWFGR HLPTEILKRY 201 L* m214/a214 99.3% identity in 152 aa overlap 10 20 30 40 50 60 m214.pep MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLDQANQSTTFSGNWIRQGTL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a214 MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLDQANQSTTFSGNWIRQGTL 10 20 30 40 50 60 70 80 90 100 110 120 m214. pep NISAARVNVTRGRQRRRIREGGRFASPLQPDIGRRQRHGARTGKQRCLFICRQHRSLNRX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a214 NISAARVNVTRGXQRRRIREGGRFASPLQPDIGRRQRHGARTGKQRCLFICRQHRSLNRX 70 80 90 100 110 120 130 140 150 m214. pep CQSTARRRCRRRCGDYIQHQNRSLYHQRQHKI I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I a214 CQSTARRRCRRRCGDYIQHQNRSLYHQRQHKIRRKIRFQIRQGQRRYPAFEYAKIRIIPM 130 140 150 160 170 180 a214 PSETXTWFGRHLPTEILKRYLX 190 200 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 745>: g214-l.seq 1 ATGATACAAA AGATATGTAA GCTATTTGTT TTAATTGTAA TTTTTGCAAC 51 TTCTCCCGCT TTTGCCCTTC AAAGCGACAG CAGACGGCCC ATCCAAATCG 101 AAGCCGACCA AGGTTCGCTC GATCAAGCCA ACCAAAGTAC CACATTTAGC 151 GGCAATGTCA TCATCAGACA GGGTACGCTC AACATTTCCG CCTCGCGCGT 201 CAACGTCACA CGCGGCGGCA AAGGCGGCGA ATCCGTGAGG GCGGAAGGTT 251 CGCCCGTCCG CTTCAGCCAA ACGTTGGACG GGGGCAAAGG GACGGTGCGC 301 GGTCAGGCAA ACAACGTTAC CTATTCCTCC GCAGGAAGCA CCGTCGTTCT 351 GACCGGCAAT GCCAAAGTGC AGCGCGGCGG CGACGTTGCC GAAGGTGCGG 401 TCATTACCTA CAACACCAAA ACCGAAGTCT ATACCATCAA CGGCAGCACG 451 AAATCGGGTG CGAAATCCGC TTCCAAAACC GGCAGGGTCA GCGTCGTCAT 501 CCAGCCTTCA AGCACACAAA AAACCGAATA A This corresponds to the amino acid sequence <SEQ ID 746; ORF 214-1. ng>: g214-l.pep 1 MIQKICKLFV LIVIFATSPA FALQSDSRRP IQIEADQGSL DQANQSTTFS 51 GNVIIRQGTL NISASRVNVT RGGKGGESVR AEGΞPVRFSQ TLDGGKGTVR 101 GQANNVTYΞS AGSTWLTGN AKVQRGGDVA EGAVITYNTK TEVYTINGST 151 KSGAKSASKT GRVSWIQPS STQKTE* The following partial DNA sequence was identified in N meningitidis <SEQ ID 747>: m214-l.seq 1 ATGATACAAA AGATATGTAA GCTATTTGTT TTAATAGCAT TTTTTTCGGC 51 GTCCCCCGCT TTTGCCCTTC AAAGCGACAG CAGGCAGCCT ATTCAGATTG 101 AGGCCGACCA AGGTTCGCTC GATCAAGCCA ACCAAAGCAC CACATTCAGC 151 GGAAACGTCG TCATCAGACA GGGTACGCTC AATATTTCCG CCGCCCGCGT 201 CAATGTTACA CGCGGCGGCA AAGGCGGCGA ATCCGTGAGG GCGGAAGGTT 251 CGCCAGTCCG CTTCAGCCAG ACATTGGACG GCGGCAAAGG CACGGTGCGC 301 GGACAGGCAA ACAACGTTGC TTATTCATCT GCAGGCAGCA CCGTAGTCTT 351 AACCGGTAAT GCCAAAGTAC AGCGCGGCGG CGATGTCGCC GAAGGTGCGG 401 TGATTACATA CAACACCAAA ACCGAAGTCT ATACCATCAG CGGCAGCACA 451 AAATCCGGCG CAAAATCCGC TTCCAAATCC GGCAGGGTCA GCGTCGTTAT 501 CCAGCCTTCG AGTACGCAAA AATCCGAATA A This corresponds to the amino acid sequence <SEQ ID 748; ORF 214-1>: m214-l .pep 1 MIQKICKLFV LIAFFSASPA FALQSDSRQP IQIEADQGSL DQANQSTTFS 51 GNWIRQGTL NISAARVNVT RGGKGGESVR AEGSPVRFSQ TLDGGKGTVR 101 GQANNVAYΞΞ AGSTWLTGN AKVQRGGDVA EGAVITYNTK TEVYTISGST 151 KSGAKSASKS GRVSWIQPS STQKSE* m214-l/g214-l 93.8% identity in 176 aa overlap 10 20 30 40 50 60 m214-l.pep MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLDQANQSTTFSGNVVIRQGTL I I I I I I I II I I I :: I :: I I I I I I I I I I I :| I I I I I I I I I I I I I I I I I I I I I I I :l I I I I I g214-l MIQKICKLFVLIVIFATSPAFALQSDSRRPIQIEADQGΞLDQANQΞTTFSGNVIIRQGTL 10 20 30 40 50 60 70 80 90 100 110 120 m214-l.pep NISAARVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQANNVAYSSAGSTVVLTGN I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I :| I I I I I I I I I I I I g214-l NISASRVNVTRGGKGGEΞVRAEGSPVRFSQTLDGGKGTVRGQANNVTYSSAGSTWLTGN 70 80 90 100 110 120 130 140 150 160 170 m214-l.pep AKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSAΞKΞGRVSWIQPSSTQKSEX I I I I I I I I I I I I I I I I I I I I I I I I I I :l I I I I I I I I I I I :l I I I I I I I I I I I I I : I I g214-l AKVQRGGDVAEGAVITYNTKTEVYTINGSTKSGAKΞASKTGRVSWIQPSSTQKTEX 130 140 150 160 170 g214-l/p38685 sp|P38685| YHBN_ECOLI 17.3 KD PROTEIN IN MURA-RPON INTERGENIC REGION PRECURSOR (0RF185) >gι 1551336 (U12684) orfl85 [Escherichia coli] >gι 1606139 (U18997) ORF_ol85 [Escherichia coli] >g I 1789592 (AE000399) orf, hypothetical protein [Escherichia coli] Length = 185 Score = 97.1 bits (238), Expect = 6e-20 Identities = 57/126 (45%), Positives = 74/126 (58%), Gaps = 3/126 (2%) Query: 19 PAFALQSDSRQPIQIEADQGSLDQANQSTTFSGNVVIRQGTLNISAARVNVTR—GGKGG 76 PAFA+ D+ QPI IE+DQ SLD TF+GNV++ QGT+ I+A +V VTR G +G Sbjct: 24 PAFAVTGDTDQPIHIEΞDQQSLDMQGNWTFTGNVIVTQGTIKINADKVWTRPGGEQGK 83 Query: 77 ESVRAEGSPVRFΞQTLDGGKGTVRGQANNVAYSSAGSTWLTGNAKVQRGGDVAEGAVIT 136 E + G P F Q D GK V G A+ + Y A VVLTGNA +Q+ +G IT Sbjct: 84 EVIDGYGKPATFYQMQDNGK-PVEGHASQMHYELAKDFVVLTGNAYLQQVDΞNIKGDKIT 142 Query: 137 YNTKTE 142 Y K + Sbjct: 143 YLVKEQ 148 The following partial DNA sequence was identified in N meningitidis <SEQ ID 749>: a214-l.seq 1 ATGATACAAA AGATATGTAA GCTATTTGTT TTAATAGCAT TTTTTTCGGC 51 GTCCCCCGCT TTTGCCCTTC AAAGCGACAG CAGGCAGCCT ATTCAGATTG 101 AGGCCGACCA AGGTTCGCTC GATCAAGCCA ACCAAAGCAC CACATTCAGC 151 GGAAACGTCG TCATCAGACA GGGTACGCTC AATATTTCCG CCGCCCGCGT 201 CAATGTTACA CGCGGCGGCA AAGGCGGCGA ATCCGTGAGG GCGGAAGGTT 251 CGCCAGTCCG CTTCAGCCAG ACATTGGACG GCGGCAAAGG CACGGTGCGC 301 GGACAGGCAA ACAACGTTGC TTATTCATCT GCAGGCAGCA CCGTAGTCTT 351 AACCGGTAAT GCCAAAGTAC AGCGCGGCGG CGATGTCGCC GAAGGTGCGG 401 TGATTACATA CAACACCAAA ACCGAAGTCT ATACCATCAG CGGCAGCACA 451 AAATCCGGCG CAAAATCCGC TTCCAAATCC GGCAGGGTCA GCGTCGTTAT 501 CCAGCCTTCG AGTACGCAAA AATCCGAATA A This corresponds to the amino acid sequence <SEQ ID 750; ORF 214-1. a>: 1 MIQKICKLFV LIAFFSASPA FALQSDSRQP IQIEADQGSL DQANQSTTFS 51 GNWIRQGTL NISAARVNVT RGGKGGESVR AEGSPVRFΞQ TLDGGKGTVR 101 GQANNVAYSS AGSTWLTGN AKVQRGGDVA EGAVITYNTK TEVYTISGST 151 KSGAKSASKS GRVSWIQPS STQKSE* a214-l/m214-l 100.0% identity in 176 aa overlap 10 20 30 40 50 60 a214-l.pep MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLDQANQSTTFSGNWIRQGTL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m214-l MIQKICKLFVLIAFFΞASPAFALQΞDΞRQPIQIEADQGΞLDQANQΞTTFSGNWIRQGTL 10 20 30 40 50 60 70 80 90 100 110 120 a214-1. pep NISAARVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQANNVAYSSAGΞTWLTGN I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m214 - 1 NI SAARVNVTRGGKGGESVRAEGΞ PVRFSQTLDGGKGTVRGQANNVAYSSAGSTVVLTGN 70 80 90 100 110 120 130 140 150 160 170 a214 - l . pep AKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKΞAΞKSGRVSWIQPSΞTQKSEX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m214-l AKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKSGRVSVVIQPSSTQKSEX 130 140 150 160 170 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 751>: g215.seq 1 atgaaagtaa gatggcggta cggaattgcg ttcccattga tattggcggt 51 tgccttgggc agcctgtcgg catggttggg ccgtatcagc gaagtcgaaa 101 tcgaggaagt caggctcaat cccgacgaac ctcaatacac aatggacggc 151 ttggacggaa ggcggtttga cgaacaggga tacttgaaag aacatttgag 201 cgcgaaaggt gcgaaacagt ttcccgaaaa cagcgacatc cattttgatt 251 cgccgcatct cgtgttcttc caagaaggca ggctgttgta cgaagtcggc 301 agcgatgaag ccgtttacca taccgaaaac aaacaggttc tttttaaaaa 351 caacgttgtg ctgaccaaaa ccgccgacgg caggcggcag gcgggtaaag 401 tcgaaaccga aaaactgcac gtcgataccg aatctcaata tgcccaaacc 451 gatacgcctg tcagtttcca atatggcgcg tcgcacggtc aggcgggcgg 501 tatgacctac aaccacaaaa caggcatgtt gaacttctca tctaaagtga 551 aagccgcgat ttatgataca aaagatatgt aa This corresponds to the amino acid sequence <SEQ ID 752; ORF 215.ng>: g215.pep 1 MKVRWRYGIA FPLILAVALG SLSAWLGRIS EVEIEEVRLΝ PDEPQYTMDG 51 LDGRRFDEQG YLKEHLSAKG AKQFPEΝSDI HFDSPHLVFF QEGRLLYEVG 101 SDEAVYHTEΝ KQVLFKΝΝW LTKTADGRRQ AGKVETEKLH VDTESQYAQT 151 DTPVSFQYGA SHGQAGGMTY ΝHKTGMLΝFS SKVKAAIYDT KDM* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 753>: m215.seq (partial) 1 ..AGCCTGTCGG CATGGTTGGG TCGTATCAGC GAAGTCGAGA TTGAAGAAGT 51 CAGGCTCAAT CCCGACGAAC CGCAATACAC AATGGACAGC TTGGACGGCA 101 GGCGGTTTGA CGAACAGGGA TACTTGAAAG AACATTTGAG CGCGAAGGGC 151 GCGAAACAGT TTCCGGAAAG CAGCGACATC CATTTTGATT CGCCGCATCT 201 CGTGTTCTTC CAAGAAGGCA GGTTGTTGTA CGAAGTCGGC AGCGACGAAG 251 CCGTTTACCA TACCGAAAAC AAACAGGTTC TTTTTAAAAA CAACGTTGTG 301 CTGACCAAAA CCGCCGACGG CAAACGGCAG GCGGGTAAAG TTGAAGCCGA 351 AAAGCTGCAC GTCGATACCG AATCTCAATA TGCCCAAACC GATACGCCTG 401 CAGTTTCCA ATATGGTGCA TCGCACGGTC AGGCGGGCGG CATGACTTAC 451 GACCACAwwA CAGGCATGTT GAACTTCTCA TCTAAAGTGA AAGCCACGAT 501 TTATGATACA AAAGATATGT AA This corresponds to the amino acid sequence <SEQ ID 754; ORF 215>: m215.pep (partial) 1 ..SLSAWLGRIS EVEIEEVRLΝ PDEPQYTMDS LDGRRFDEQG YLKEHLSAKG 51 AKQFPESSDI HFDSPHLVFF QEGRLLYEVG SDEAVYHTEΝ KQVLFKΝΝW 101 LTKTADGKRQ AGKVEAEKLH VDTESQYAQT DTPVSFQYGA SHGQAGGMTY 151 DHXTGMLNFS SKVKATIYDT KDM* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 215 shows 96.0% identity over a 173 aa overlap with a predicted ORF (ORF 215.ng) from N gonorrhoeae: m215/g215 10 20 30 40 m215.pep SLSAWLGRISEVEIEEVRLΝPDEPQYTMDSLDGRRFDEQG g215 MKVRWRYGIAFPLI AVALGSLSAWLGRISEVEIEEVRLΝPDEPQYTMDGLDGRRFDEQG 10 20 30 40 50 60 50 60 70 80 90 100 m215. pep YLKEHLSAKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSDEAVYHTEΝKQVLFKΝΝW g215 YLKEHLSAKGAKQFPEΝSDIHFDSPHLVFFQEGRLLYEVGSDEAVYHTEΝKQVLFKΝΝW 70 80 90 100 110 120 110 120 130 140 150 160 m215. pep LTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGASHGQAGGMTYDHXTGMLΝFS III II II lllhl g215 LTKTADGRRQAGKVETEKLHVDTESQYAQTDTPVSFQYGASHGQAGGMTYΝHKTGMLΝFS 130 140 150 160 170 180 170 m215.pep SKVKATIYDTKDMX g215 SKVKAAIYDTKDM 190 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 755>: a215. seq 1 ATGAAAGTAA GATGGCGGTA CGGAATTGCG TTCCCATTGA TATTGGCGGT 51 TGCCTTGGGC AGCCTGTCGG CATGGTTGGG ACGCATCAGC GAAGTCGAGA 101 TTGAAGAAGT CAGGCTCAAT CCCGACGAAC CGCAATACAC AATGGACGGA 151 TTGGATGGCA GGCGGTTTGA CGAACAGGGA TACTTGAAAG AACATTTGAG 201 TTCGAAGGGC GCGAAACAGT TTCCCGAAAG CAGCGACATT CATTTCGACT 251 CACCGCATCT CGTGTTCTTC CAAGAAGGCA GGTTGTTGTA CGAAGTCGGC 301 AGCGATGAAG CCGTTTACCA TACCGAAAAC AAACAGGTTC TTTTTAAAAA 351 CAACGTTGTG CTGACCAAAA CCGCCGACGG CAAACGGCAG GCGGGTAAAG 401 TTGAAGCCGA AAAGCTGCAC GTCGATACCG AATCTCAATA TGCCCAAACC 451 GATACGCCTG TCAGTTTCCA ATATGGTGCA TCGCACGGTC AGGCGGGCGG 501 CATGACTTAC GACCACAAAA CAGGCATGTT GAACTTCTCA TCTAAAGTGA 551 AAGCCACGAT TTATGATACA AAAGATATGT AA This corresponds to the amino acid sequence <SEQ ID 756; ORF 215. a>: a215.pep 1 MKVRWRYGIA FPLILAVALG SLSAWLGRIS EVEIEEVRLΝ PDEPQYTMDG 51 LDGRRFDEQG YLKEHLSSKG AKQFPESSDI HFDSPHLVFF QEGRLLYEVG 101 SDEAVYHTEΝ KQVLFKΝΝVV LTKTADGKRQ AGKVEAEKLH VDTESQYAQT 151 DTPVSFQYGA SHGQAGGMTY DHKTGMLΝFS SKVKATIYDT KDM* m215/a215 98.3% identity in 173 aa overlap 10 20 30 40 m215.pep SLSAWLGRISEVEIEEVRLΝPDEPQYTMDSLDGRRFDEQG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I II I I I I a215 MKVRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLΝPDEPQYTMDGLDGRRFDEQG 10 20 30 40 50 60 50 60 70 80 90 100 m215.pep YLKEHLSAKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSDEAVYHTEΝKQVLFKΝΝW I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a215 YLKEHLSSKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSDEAVYHTENKQVLFKNNW 70 80 90 100 110 120 110 120 130 140 150 160 m215. pep LTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGASHGQAGGMTYDHXTGMLNFS I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I lllllll a215 LTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGASHGQAGGMTYDHKTGMLNFS 130 140 150 160 170 180 170 m215.pep SKVKATIYDTKDMX I I I I I I I I I I I I I I a215 SKVKATIYDTKDMX 190 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ TD 757>: g216.seq (partial) 1 ..atgatatcga tttcgagctc ggtacccagc gacgaaatca ccgccatcat 51 ccccgcactc aaacgcaaag acattaccct cgtctgcatc accgcccgcc 101 ccgattcaac catggcgcgc catgccgata tccacatcac cgcatcggtt 151 tcgcaagaag cctgcccgtt ggggcttgcc ccgaccacca gcaccaccgc 201 cgttatggct ttgggcgacg cgttggcggt cgtcctgctg cgcgcccgcg 251 cgttcacgcc cgacgacttc gccttgatcc accctgccgg cagcctcggc 301 aaacgcctgc ttttgcgcgt tgccgacatt atgcacaaag gcggcggcct 351 gcccgccgtc cgactcggca cgcccttgaa aggagccatc gtcagcatga 401 gcgagaaagg tttgggcatg tgggcgggaa cggacgggca aaggctgtct 451 gaaaggcctt tttactga This corresponds to the amino acid sequence <SEQ ID 758; ORF 216.ng>: g216.pep (partial) 1 ..MISISSSVPS DEITAIIPAL KRKDITLVCI TARPDSTMAR HADIHITASV 51 SQEACPLGLA PTTSTTAVMA LGDALAWLL RARAFTPDDF ALIHPAGSLG 101 KRLLLRVADI MHKGGGLPAV RLGTPLKGAI VSMSEKGLGM WAGTDGQRLS 151 ERPFY* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 759>: m216. seq 1 ATGGCAATGG CAGAAAACGG AAAATATCTC GACTGGGCAC GCGAAGTGTT 51 GCACGCCGAA GCGGAAGGCT TGCGCGAAAT TGCAGCGGAA TTGsACAAAA 101 ACTTCGTCCT TGCGGCAGAC GCGTTGTTGC ACTGCAAGGG CAGGGTCGTT 151 ATCACGGGCA TGGTCAAGTC GGGACATATC GGGCGCAAAA TGGCGGCAAC 201 TATGGCCTCG ACCGGCACGC CTGCGTTTTT CGTCCACCCT GCGGAAGCGG 251 CACACGgCGA TTTGGGTATG ATTGTGGACA rCGACGTGGT CGTCGCGATT 301 TCCAATTCCG GCGAAAGCGA CGAAATCGCC GCCATCATCC CCGCACTCAA 351 ACGCAAAGAC ATCACGCTTG TCTGCATCAC CGCCCGCCCC GATTCAACCA 401 TGGCGCGCCA TGCCGACATC CACATCACGG CGTCGGTTTC CAAAGAAGCC 451 TGCCCGCTGG GGCTTGCCCC GACCACCAGC ACCACCGCCG TCATGGCTTT 501 GGGCGATGCG TTGGCGGTCG TCCtGCTGCG CgcACGCGCG TTCACGCCCG 551 ACGATTTCGC CTTGAGCCAT CCTGCCGGCA GCCTCGGCAA ACGCCTACTT 601 TTGCGCGTTG CCGACATTAT GCACAAAGGC GGCGGCCTGC CTGCCGTCCG 651 ACTCGGCACG CCCTTGAAAG AAGCCATCGT CAGCATGAGT GAAAAAGGGC 701 TGGGCATGTT GGCGGTAACG GACGGGCAAG GCCGTCTGAA AGGCGTATTC 751 ACCGACGGCG ATTTGCGCCG CCTGTTTCAA GAATGCGACA ATTTTACCGG 801 TCTTTCGATA GACGAAGTCA TGCATACGCA TCCTAAAACC ATCTCCGCCG 851 AACGTCTCGC CACCGAAGCC CTGAAAGTCA TGCAGGCAAA CCATGTGAAC 901 GGGCTTCTGG TTACCGATGC AGATGGCGTG CTGATCGGCG CGCTGAATAT 951 GCACGACCTG CTGGCGGCAC GGATTGTATA G This corresponds to the amino acid sequence <SEQ ID 760; ORF 216>: m2l6.pep 1 MAMAEΝGKYL DWAREVLHAE AEGLREIAAE LXKΝFVLAAD ALLHCKGRW 51 ITGMVKSGHI GRKMAATMAS TGTPAFFVHP AEAAHGDLGM IVDXDVWAI 101 SNSGESDEIA AIIPALKRKD ITLVCITARP DSTMARHADI HITASVSKEA 151 CPLGLAPTTS TTAVMALGDA LAWLLRARA FTPDDFALSH PAGSLGKRLL 201 LRVADIMHKG GGLPAVRLGT PLKEAIVSMS EKGLGMLAVT DGQGRLKGVF 251 TDGDLRRLFQ ECDNFTGLSI DEVMHTHPKT ISAERLATEA LKVMQANHVN 301 GLLVTDADGV LIGALNMHDL LAARIV* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N. gonorrhoeae ORF 216 shows 91.8% identity over a 147 aa overlap with a predicted ORF (ORF 216.ng) from N gonorrhoeae: m216/g216 70 80 90 100 110 120 m216.pep TMASTGTPAFFVHPAEAAHGDLGMIVDXD WAISΝSGESDEIAAIIPALKRKDITLVCI :: hl g216 MISISSSVPSDEITAIIPALKRKDITLVCI 10 20 30 130 140 150 160 170 180 m216.pep TARPDSTMARHADIHITASVSKEACPLGLAPTTSTTAVMALGDALAWLLRARAFTPDDF g216 TARPDSTMARHADIHITASVSQEACPLGLAPTTSTTAVMALGDALAWLLRARAFTPDDF 40 50 60 70 80 90 190 200 210 220 230 240 m216.pep ALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGTPLKEAIVSMSEKGLGMLAVTDGQGRL II I MM g216 ALIHPAGSLGKRLLLRVADIMHKGGGLPAVRLGTPLKGAIVSMSEKGLGMWAGTDGQRLS 100 110 120 130 140 150 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 761>: a216. seq 1 ATGGCGATGG CAGGAAACGA AAAATATCTT GATTGGGCAC GCGAAGTGTT 51 GCACACCGAA GCGGAAGGCT TGCGCGAAAT TGCGGCGGAT TTGGACGAAA 101 ACTTCGCCCT TGCGGCGGAC GCGTTGTTGC ACTGCAAAGG CAGGGTCGTT 151 ATCACGGGCA TGGGCAAGTC GGGACATATC GGGCGCAAAA TGGCGGCAAC 201 CATGGCCTCG ACCGGCACGC CCGCGTTTTT CGTCCACCCT GCGGAAGCGG 251 CACACGGCGA TTTGGGCATG ATTGTGGACA ACGACGTGGT CGTCGCGATT 301 TCCAATTCCG GTGAAAGCGA CGAAATCGCC GCCATCATCC CCGCGCTCAA 351 ACGCAAAGAT ATCACGCTTG TCTGCATCAC CGCCCGCCCC GATTCAACCA 401 TGGCGCGCCA TGCCGACATC CACATCACGG CGTCGGTTTC CAAAGAAGCC 451 TGCCCGCTGG GGCTTGCCCC GACCACCAGC ACCACCGCCG TTATGGCTTT 501 GGGCGATGCG TTGGCGGTTG TCCTGCTGCG CGCCCGCGCG TTCACGCCCG 551 ACGACTTCGC CTTGAGCCAC CCTGCCGGCA GCCTCGGCAA ACGCCTACTT 601 TTGCGCGTTG CCGACATTAT GCACAAAGGC GGCGGCCTGC CTGCCGTCCG 651 ACTCGGCACG CCCTTGAAAG AAGCCATCGT CAGCATGAGT GAAAAAGGGC 701 TGGGCATGTT GGCGGTAACG GACGGGCAAG GCCGTCTGAA AGGCGTATTC 751 ACCGACGGCG ATTTGCGCCG CCTGTTTCAA GAATGCGACA ATTTTACCGG 801 TCTTTCGATA GACGAAGTCA TGCATACGCA TCCTAAAACC ATCTCCGCCG 851 AACGTCTCGC CACCGAAGCC CTGAAAGTCA TGCAGGCAAA CCATGTGAAC 901 GGGCTTCTGG TTACCGATGC AGATGGCGTG CTGATCGGCG CGCTGAATAT 951 GCACGACCTT TTGGCGGCGC GGATTGTATA G This corresponds to the amino acid sequence <SEQ ID 762; ORF 216.a>: a216.pep 1 MAMAGΝEKYL DWAREVLHTE AEGLREIAAD LDEΝFALAAD ALLHCKGRW 51 ITGMGKSGHI GRKMAATMAS TGTPAFFVHP AEAAHGDLGM IVDΝDVWAI 101 SNSGESDEIA AIIPALKRKD ITLVCITARP DSTMARHADI HITASVSKEA 151 CPLGLAPTTS TTAVMALGDA LAWLLRARA FTPDDFALSH PAGSLGKRLL 201 LRVADIMHKG GGLPAVRLGT PLKEAIVSMS EKGLGMLAVT DGQGRLKGVF 251 TDGDLRRLFQ ECDNFTGLSI DEVMHTHPKT ISAERLATEA LKVMQANHVN 301 GLLVTDADGV LIGALNMHDL LAARIV* m216/a216 97.2% identity in 326 aa overlap 10 20 30 40 50 60 m216.pep MAMAENGKYLDWAREVLHAEAEGLREIAAELXKNFVLAADALLHCKGRWITGMVKSGHI I I I I I I I I I I I I I I I I: I I I I I I I I I I : I : I I : I I I I I I I I I I I I I I I I II I I I I I a216 MAMAGNEKYLDWAREVLHTEAEGLREIAADLDENFALAADALLHCKGRWITGMGKSGHI 10 20 30 40 50 60 70 80 90 100 110 120 m216.pep GRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDXDVWAISNSGESDEIAAIIPALKRKD I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a216 GRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVWAISNSGESDEIAAIIPALKRKD 70 80 90 100 110 120 130 140 150 160 170 180 m216.pep ITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTTAVMALGDALAWLLRARA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a216 ITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTTAVMALGDALAWLLRARA 130 140 150 160 170 180 190 200 210 220 230 240 m216.pep FTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGTPLKEAIVSMSEKGLGMLAVT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a216 FTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGTPLKEAIVSMSEKGLGMLAVT 190 200 210 220 230 240 250 260 270 280 290 300 m216. pep DGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHPKTISAERLATEALKVMQANHVN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a216 DGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHPKTISAERLATEALKVMQANHVN 250 260 270 280 290 300 310 320 m216. pep GLLVTDADGVLIGALNMHDLLAARIVX I I I I I I I I I I I I I I I I I I I I I I I I I I I a216 GLLVTDADGVLIGALNMHDLLAARIVX 310 320 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 763>: g217.seq 1 atggcggatg acggtttgtt gcggcaactg tccgaaaaac ccagccaaag 51 tgctctcttc ctgccatttg acccattcgt tttcgaggtt ttggactgcc 101 ttttggtcat cgggcccggc ttgaaacaat gtttcaagca aatcccggca 151 acgcgccacc cattcgccga ccgtcgcagg ttgccgccat atccgggcaa 201 tatccgacag ggtttcgagg aaggcggcaa aacgtccgaa catggcggtt 251 tgattcacgt cggcatacca cgcgctgaca tcctgccaca tcgggttgcc 301 gccttcgggc agcatccagc ccaatatcat acggtctgcc gcctgcttcc 351 aggtaaacag ctgatccgtg ccgccgcgca tttctccgtc caatccccaa 401 tggacgttca aatcggcaac catatcgtgc aaaagcggca aatcgtcccc 451 ggtcagtccg aaacggcgca acacgggcgc ggtttccaaa agcgcgagca 501 ctttgccgac ttcaaaacgg ctttccagca agtcggacac gcactccaac 551 gcataaaaaa acggttgccg gcggctgatt ttcacgtccg aaacggaata 601 cggcaatgcc tgcgcgccgg gttgcgcctg tccgaacacg gcttccataa 651 aaggcgtata gggttcgata ttcggggtta a This corresponds to the amino acid sequence <SEQ ID 764; ORF 217.ng>: g217.pep.. 1 MADDGLLRQL SEKPSQSALF LPFDPFVFEV LDCLLVIGPG LKQCFKQIPA 51 TRHPFADRRR LPPYPGΝIRQ GFEEGGKTSE HGGLIHVGIP RADILPHRVA 101 AFGQHPAQYH TVCRLLPGKQ LIRAAAHFSV QSPMDVQIGΝ HIVQKRQIVP 151 GQSETAQHGR GFQKREHFAD FKTAFQQVGH ALQRIKKRLP AADFHVRΝGI 201 RQCLRAGLRL SEHGFHKRRI GFDIRG* The following partial DNA sequence was identified in N meningitidis <SEQ ID 765>: m217.seq 1 ATGGCGGATG ACGGTGTGCG GCGGCAACTG TCCGGAAAAT TGCGCCAATT 51 CGGTTTCCGC CTrCCATTTG ACCCATTCGT TTTCAAGGTT TTGGACTGAC 101 TTTTGGTCAT CGGCTTCAGC TTGGAACAAT GTTTCAAGCA AATCCCGGCA 151 ACGCGCCACC CATTCGCCGA CCGTTGCGGG CTGCCGCCAT ATCCGTACAA 201 TATCCGTCAG GGTTTCGAGG AAGGCGGCAA AACGTCCGAA CATGGCGGTT 251 TGATTCACGT CGGCATACCA CGCGCTGACA TCCTGCCACA TCGGATTGCC 301 GCCTTTGGGC AGCATCCAGC CCAATATCAT GCGTTCTACC GCCTGCTTCC 351 AGGTGAACAG CTGATCCGTG CCGCCGCGCA TTTCTCCGTC CAAACCCCAG 401 TGGACGTTCA AATCGGCAAC CATGTCGTGC AAAAGCGGTA AATCGTCCTC 451 AGTCAGTCCG AAACGGCGCA ACACGGGCGC GGTTTCTAAA AGCACAAGCA 501 CTTTATCGAC TTCAAATCGG CTTTCCAACA AGTCGAACAG GCATGACAAA 551 GCATGAAACA GCGGTTGGCG GCGGCTGATT TTCACGTCTG ACACGGAATA 601 CGGCAATGCC TGCGCACCgG GctGCGCCTG TCCGAACACG GCTTCGATAA 651 AAGGCGTATA GGATTCGATA TTCGGGGTTA A This corresponds to the amino acid sequence <SEQ ID 766; ORF 217>: m217.pep 1 MADDGVRRQL SGKLRQFGFR LPFDPFVFKV LDXLLVIGFS LEQCFKQIPA 51 TRHPFADRCG LPPYPYΝIRQ GFEEGGKTSE HGGLIHVGIP RADILPHRIA 101 AFGQHPAQYH AFYRLLPGEQ LIRAAAHFSV QTPVDVQIGΝ HWQKRXIVL 151 SQSETAQHGR GFXKHKHFID FKSAFQQVEQ AXQSMKQRLA AADFHVXHGI 201 RQCLRTGLRL SEHGFDKRRI GFDIRG* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 217 shows 80.5% identity over a 226 aa overlap with a predicted ORF (ORF 217.ng) from N gonorrhoeae: m217/g217 10 20 30 40 50 60 m217.pep MADDGVRRQLSGKLRQFGFRLPFDPFVFKVLDXLLVIGFSLEQCFKQIPATRHPFADRCG l l l l h M M I I ; : M i l l = h I I 1 1 I I I I I I I 1 1 1 1 1 g217 MADDGLLRQLSEKPSQSALFLPFDPFVFEVLDCLLVIGPGLKQCFKQIPATRHPFADRRR 10 20 30 40 50 60 70 80 90 100 110 120 m217.pep LPPYPYΝIRQGFEEGGKTSEHGGLIHVGIPRADILPHRIAAFGQHPAQYHAFYRLLPGEQ M i l l l l l l h l g217 LPPYPGΝIRQGFEEGGKTSEHGGLIHVGIPRADILPHRVAAFGQHPAQYHTVCRLLPGKQ 70 80 90 100 110 120 130 140 150 160 170 180 m217 . pep LIRAAAHFSVQTPVDVQIGΝHWQKRXIVLSQSETAQHGRGFXKHKHFIDFKSAFQQVEQ I I M M I M I M M h M I l l h l l l l l = g217 LIRAAAHFSVQSPMDVQIGΝHIVQKRQIVPGQSETAQHGRGFQKREHFADFKTAFQQVGH 130 140 150 160 170 180 190 200 210 220 m217.pep AXQSMKQRLAAADFHVXHGIRQCLRTGLRLSEHGFDKRRIGFDIRGX I I = h l l g217 ALQRIKKRLPAADFHVRΝGIRQCLRAGLRLSEHGFHKRRIGFDIRG 190 200 210 220 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 767>: a217. seq 1 GTGGCGGATG ACGGTGTGCA GCGGCAACTG TCCGGAAAAT TGCGCCAATT 51 CGGTTTCCGC CTGCCATTTG ACCCATTCGT TTTCGAGGCT TTGGACTGCC 101 TTTTGGTCAT CGCCTTCGAC TTGGAACAAT GTTTCAAGCA AATCCCGGCA 151 ACGCGCCACC CATTCGTCAA CCGTCGCAGG TTGCCGCCAT ATCCGTACAA 201 TATCCGTCAG GGTTTCGAGG AAGGCGGCAA AACGTCCGAA CAGGGCGGTT 251 TGGTTCACGT CGGCATACCA CGCGCTGACC CCCTGCCACA TCGGATTGCC 301 GCCTTCGGGC AGCATCCAGC CCAATATCAT GCGTTCTACC GCCTGCTTCC 351 AGGTGAACAG CTGATCCGTG CCGCCGCGCA TTTCTCCGTC CAAACCCCAG 401 CGGACGTTCA AATCGGCAAC CATGTCGTGC AAAAGCGGCA AATCGTCCTC 451 AGTCAGTCCG AAATGGCGCA ACACGGGCGC GGTTTCTAAA AGCACAAGCA 501 CTTTATCGAC TTCAAATCGG CTTTCCAACA AGTCGAACAG GCATGACAAA 551 GCATGAAACA GCGGTTGTCG GCGGCTGATT TTCACATCCG AAACGGAATA 601 CGGCAATGCC TGCGCGCCGG GCTGCGCCTG TCCGAACACG GCTTCGATAA 651 AAGGCGTATA GGATTCGATA TTCGGGGTTA A This corresponds to the amino acid sequence <SEQ ID 768; ORF 217.a>: a217.pep 1 VADDGVQRQL SGKLRQFGFR LPFDPFVFEA LDCLLVIAFD LEQCFKQIPA 51 TRHPFVNRRR LPPYPYNIRQ GFEEGGKTSE QGGLVHVGIP RADPLPHRIA 101 AFGQHPAQYH AFYRLLPGEQ LIRAAAHFSV QTPADVQIGN HWQKRQIVL 151 SQSEMAQHGR GF*KHKHFID FKSAFQQVEQ A*QSMKQRLS AADFHIRNGI 201 RQCLRAGLRL SEHGFDKRRI GFDIRG* m217/a217 90.3% identity in 226 aa overlap 10 20 30 40 50 60 m217.pep MADDGVRRQLSGKLRQFGFRLPFDPFVFKVLDXLLVIGFΞLEQCFKQIPATRHPFADRCG :| I I I I : I I I I I I I I I I I I I I I I I I I I I ::l I I I I I : I : I I I I I I I I I I I I I I I :: I a217 VADDGVQRQLSGKLRQFGFRLPFDPFVFEALDCLLVIAFDLEQCFKQIPATRHPFVNRRR 10 20 30 40 50 60 70 80 90 100 110 120 m217.pep LPPYPYNIRQGFEEGGKTΞEHGGLIHVGIPRADILPHRIAAFGQHPAQYHAFYRLLPGEQ I I I I I I I I I I I I I I I I I I I I :| I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a217 LPPYPYNIRQGFEEGGKTSEQGGLVHVGIPRADPLPHRIAAFGQHPAQYHAFYRLLPGEQ 70 80 90 100 110 120 130 140 150 160 170 180 m217 . pep LIRAAAHFSVQTPVDVQIGNHWQKRXIVLSQSETAQHGRGFXKHKHFIDFKSAFQQVEQ I I I I I I I I I I I I I : | I I I I I I I I I I I l l l l l l l I I I I I I I I I I I I I I I I I I I I I I I I I a217 LIRAAAHFΞVQTPADVQIGNHVVQKRQIVLΞQSEMAQHGRGFXKHKHFIDFKSAFQQVEQ 130 140 150 160 170 180 190 200 210 220 m217.pep AXQΞMKQRLAAADFHVXHGIRQCLRTGLRLSEHGFDKRRIGFDIRGX I I I I I I I I 1:1 I I I I : : I I I I I I I : I I I II I I I I I I I I I I I I I I I I a217 AXQSMKQRLΞAADFHIRNGIRQCLRAGLRLSEHGFDKRRIGFDIRGX 190 200 210 220 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 769>: g2l8.seq 1 atggttgcgg tggatcctta tacggcaaaa gtggtcaaca ccatgccgcg 51 caatcagggt tggtatcaca ctatggatga aatccacggc gatatgatgc 101 tcggtgcggc aggcgattat cttttggaaa cggcagcttc actgaccatt 151 attatggttg tcagcggctt gtacctttgg tgggcgaaac agcgcggcat 201 taaagcgatg ctgctgccgc caaaaagcag ggcgcgttct tggtggcgga 251 atctgcacgg cgcgtttgga acttgggtgt cgttgatttt actgttgttc 301 tgcctgtcgg gtattgcttg ggcaggtatt tggggcggca aattcgtgca 351 ggcttggaat cagttcccgg ccggcaaatg gggtgtcgaa ccgaaccccg 401 tttcaatcgt gccgacccac ggcgaggtat tgaatgacgg caaggttaag 451 gaagtgccgt ggattttgga gcttatgcct atgcctgtct cagggacgac 501 tgtgggtgaa aacggcatta accccaccga gcccaataac attggaaacc 551 gtcgaccgtt tcgcgcggga aatcggtttc aaagggcgtt atcagttgaa 601 tttgcccaaa ggcgaggacg gggtatggac tttgtcgcag gattctatga 651 gttatga This corresponds to the amino acid sequence <SEQ ID 770; ORF 218.ng>: g218 . pep 1 MVAVDPYTAK WΝTMPRΝQG WYHTMDEIHG DMMLGAAGDY LLETAASLTI 51 IMWSGLYLW WAKQRGIKAM LLPPKSRARS WWRNLHGAFG TWVSLILLLF 101 CLSGIAWAGI WGGKFVQAWN QFPAGKWGVE PNPVSIVPTH GEVLNDGKVK 151 EVPWILELMP MPVSGTTVGE NGINPTEPNN IGNRRPFRAG NRFQRALSVE 201 FAQRRGRGMD FVAGFYEL* The following partial DNA sequence was identified in N meningitidis <SEQ ID 771>: m218.seq 1 ATGGTCGCGG TCGATCCTTA TACGGCAAAA GTGGTCAGTA CCATGCCGCG 51 CAATCAGGGT TGGTATTACA CGATGGATGA AATCCACAGC GATATGATGC 101 TCGGTGCGGC AGGCGATTAT CTTTTGGAAA CGGCAGCTTC ACTGACCATT 151 ATTATGGTTG TCAGCGGCTT GTACCTTTGG TGGGTGAAAC GGCGCGGCAT 201 CAAGGCGATG CTGCTGCCGT CAAAAGGCAr GGCGCGTTCT TGGTGGCGGA 251 ATCTGCACGG CACGTTTGGA ACTTGGGTGT CGTTGATTTT GCTGTTGTTC 301 TGCCTGTCGG GTATTGCTTG GGCGGGTATT TGGGGCGGCA AGTTCGTACA 351 GGCTTGGAGT CAGTTCCCTG CCGGTAAATG GGGTGTCGAA CCGAACCCCG 401 TTTCAGTCGT GCCGACCCAC GGCGAGGTAT TGAATGACGG CAAGGTTAAG 451 GAAGTGCCGT GGGTTTTGGA GCTTACGCCT ATGCCTGTTT CAGGGACGaC 501 yGtgGGCAAA GACGGCATTA ACCCTGACGA GCCGATGACA TTGGAAACCG 551 TCGACCGCTT TGCGCGGnGA AATCGGTTTC AAAGGGCGTT ATCAGTTGAA 601 TTTGCCCAAA GGCGAGGACG GCGTATGGAC TTTGTCGCAG GATTCTATGA 651 GTTA This corresponds to the amino acid sequence <SEQ ID 772; ORF 218>: m218. pep 1 MVAVDPYTAK WSTMPRΝQG WYYTMDEIHS DMMLGAAGDY LLETAASLTI 51 IMWSGLYLW WVKRRGIKAM LLPSKGXARS WWRΝLHGTFG TWVSLILLLF 101 CLSGIAWAGI WGGKFVQAWS QFPAGKWGVE PΝPVSWPTH GEVLΝDGKVK 151 EVPWVLELTP MPVSGTTVGK DGIΝPDEPMT LETVDRFARX NRFQRALSVE 201 FAQRRGRRMD FVAGFYEL Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 218 shows 87.2% identity over a 218 aa overlap with a predicted ORF (ORF 218.ng) from N gonorrhoeae: m218/g218 10 20 30 40 50 60 m218 . pep MVAVDPYTAKWSTMPRΝQGWYYTMDEIHSDMMLGAAGDYLLETAASLTI IMWSGLYLW g218 MVAVDPYTAKWΝTMPRΝQGWYHTMDEIHGDMMLGAAGDYLLETAASLTI IMWSGLYLW 10 20 30 40 50 60 70 80 90 100 110 120 m218. pep WVKRRGIKAMLLPSKGXARSWWRΝLHGTFGTWVSLILLLFCLSGIAWAGIWGGKFVQAWS h g218 WAKQRGIKAMLLPPKSRARSWWRΝLHGAFGTWVSLILLLFCLSGIAWAGIWGGKFVQAWΝ 70 80 90 100 110 120 130 140 150 160 170 180 m218 . pep QFPAGKWGVEPΝPVSWPTHGEVLΝDGKVKEVPWVLELTPMPVSGTTVGKDGIΝPDEPMT M M M M M M M h l M M M M M M M M h l M M M M M I h M I M I I = g218 QFPAGKWGVEPΝPVSIVPTHGEVLΝDGKVKEVPWILELMPMPVSGTTVGEΝGIΝPTEPΝΝ 130 140 150 160 170 180 190 200 210 m218. pep LETVDRFARXΝRFQRALSVEFAQRRGRRMDFVAGFYEL = = I g218 IGΝRRPFRAGΝRFQRALSVEFAQRRGRGMDFVAGFYEL 190 200 210 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 773>: a21δ.seg 1 ATGGTCGCGG TCGATCCTTA TACGGCAAAA GTGGTCAGTA CCATGCCGCG 51 CAATCAGGGT TGGTATTACG CGATGGATGA AATCCACAGC GATATGATGC 101 TCGGTTCGAC AGGTGATTAT CTTTTGGAAA CGGCTGCATC GCTGACGATT 151 ATCATGATAA TCAGCGGTTT GTACCTTTGG TGGGTGAAAC GGCGCGGCAT 201 CAAGGCGATG CTGCTGCCGC CAAAAGGCAG GGCGCGTTCT TGGTGGCGGA 251 ATCTGCACGG CGCGTTTGGA ACTTGGGTGT CGTTGATTTT ACTGTTGTTC 301 TGCCTGTCGG GTATTGCTTG GGCAGGTATT TGGGGCGGCA AGTTCGTGCA 351 GGCTTGGAGT CAGTTCCCGG CAGGCAAATG GGGTGTCGAA CCGAACCCTG 401 TTTCAGTCGT GCCGACCCAC GGCGAGGTAT TGAATGACGG CAAGGTTAAG 451 GAAGTGCCGT GGGTTTTGGA GCTTACGCCT ATGCCTGTTT CAGGGACGAC 501 TGTGGGCAAA GACGGTATTA ACCCTGACGA GCCGATGACA TTGGAAACCG 551 TCGACCGTTT TGCGCGG . GA AATCGGTTTC AAAGGGCGTT ATCAGCTGAA 601 TTTGCCCAAA GGCGAGGACG GCGTATGGAC TTTGTCGCAG GATTCTATGA 651 GTTA This corresponds to the amino acid sequence <SEQ ID 774; ORF 218.a>: a218.pep 1 MVAVDPYTAK WSTMPRNQG WYYAMDEIHS DMMLGSTGDY LLETAASLTI 51 IMIISGLYLW WVKRRGIKAM LLPPKGRARS WWRNLHGAFG TWVSLILLLF 101 CLSGIAWAGI WGGKFVQAWS QFPAGKWGVE PNPVSWPTH GEVLNDGKVK 151 EVPWVLELTP MPVSGTTVGK DGINPDEPMT LETVDRFARX NRFQRALSAE 201 FAQRRGRRMD FVAGFYEL m218/a218 95.9% identity in 218 aa overlap 10 20 30 40 50 60 m218 . pep MVAVDPYTAKWΞTMPRNQGWYYTMDEIHSDMMLGAAGDYLLETAASLTI IMWSGLYLW I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I : : | I I I I I I I I I I I I I l : : | I I I I I a218 MVAVDPYTAKWSTMPRNQGWYYAMDEIHSDMMLGSTGDYLLETAAΞLTIIMIIΞGLYLW 10 20 30 40 50 60 70 80 90 100 110 120 m218. ■pep WVKRRGIKAMLLPSKGXARSWWRNLHGTFGT VΞLILLLFCLSGIA AGIWGGKFVQAWS I I I I I I I I I I I I I II I I I I I I I I I I :l I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a218 VKRRGIKAMLLPPKGRARSWWRNLHGAFGTWVSLILLLFCLSGIAWAGIWGGKFVQAWS 70 80 90 100 110 120 130 140 150 160 170 180 m218. .pep QFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWVLELTPMPVSGTTVGKDGINPDEPMT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a218 QFPAGKWGVEPNPVSWPTHGEVLNDGKVKEVP VLELTPMPVSGTTVGKDGINPDEPMT 130 140 150 160 170 180 190 200 210 m218. .pep LETVDRFARXNRFQRALSVEFAQRRGRRMD FVAGFYEL I I I I I I I I I I I I I I I I I I : | I I I I I I I I I I I I I I I I I I a218 LETVDRFARXNRFQRALSAEFAQRRGRRMDFVAGFYEL 190 200 210 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ED 775>: g219.seq 1 atgacggcaa ggttaaggaa gtgccgtgga ttttggagct tatgcctatg 51 cctgtctcag ggacgactgt gggtgaaaac ggcattaacc ccaccgagcc 101 caataacatt ggaaaccgtc gaccgtttcg cgcgggaaat cggtttcaaa 151 gggcgttatc agttgaattt gcccaaaggc gaggacgggg tatggacttt 201 gtcgcaggat tctatgagtt atgacatgat cagcccgttt gccgaccgca 251 cggtacatat cgaccagtac agcggcgaga ttcttgccga catccgtttt 301 gacgattaca acccgttcgg caaatttatg gcggcaagca ttgcgctgca 351 tatggggact ttgggctggt ggagcgtgtt ggcgaacgtc gtgttctgcc 401 ttgccgtgat ttttatcggc atcagcggct gcgtgatgtg gtggaaacgc 451 cgtccgtccg gcgtggcggg cattgttcct ccggcgcaaa aaatcaaact 501 gcccgtctgg tgggcgatgg cattgccgct gctgttgatt gcactgcttt 551 tcccgaccgc gctgcttgcc attgccgtga tttggctgtt ggataccttg 601 ctgctgtcgc ggattcctgt gttgaggaaa tggtttaaat ga This corresponds to the amino acid sequence <SEQ ID 776; ORF 219.ng>: g219.pep 1 MTARLRKCRG FWSLCLCLSQ GRLWVKTALT PPSPITLETV DRFAREIGFK 51 GRYQLΝLPKG EDGVWTLSQD SMSYDMISPF ADRTVHIDQY SGEILADIRF 101 DDYΝPFGKFM AASIALHMGT LGWWSVLAΝV VFCLAVIFIG ISGCVMWWKR 151 RPSGVAGIVP PAQKIKLPVW WAMALPLLLI ALLFPTALLA IAVIWLLDTL 201 LLSRIPVLRK WFK* The following partial DΝA sequence was identified in N meningitidis <SEQ ED 777>: m219. seq 1 ATGACGGCAA GGTTAAGGAA GTGCCGTGGG TTTTGGAGCT TACGCCTATG 51 CCTGTTTCAG GGACGaCyGt gGGCAAAGAC GGCATTAACC CTGACGAGCC 101 GATGACATTG GAAACCGTCG ACCGCTTTGC GCGGnGAAAT CGGTTTCAAA 151 GGGCGTTATC AGTTGAATTT GCCCAAAGGC GAGGACGGCG TATGGACTTT 201 GTCGCAGGAT TCTATGAGTT ACGACATGAT CAGCCCGTTT GCCGACCGCA 251 CGGTACATAT CGACCAGTAC AGCGGCAAAA TCCTTGCCGA CATCCGTTTT 301 GACGATTACA ACCCGTTCGG CAAATTTATG GCGGCAAGCA TTGCGCTGCA 351 TATGGGGACT CTGGGCTGGT GGAGCGTGTT GGCGAACGTC TTGTTCTGCC 401 TTGCCGTCAT TTTTATCGGT ATCAGCGGCT GCGTGATGTG GTGGAAACGC 451 CGTCCGACCG GAGCGGTGGG CATCGTTCCG CCGGCGCAGA AAGTCAAGCT 501 GCCGGTTTGG TGGATGATGG CATTGCCGCT ATTGGCAATC GCACTGCTCT 551 TCCCGACCTC ACTGCTTGCC ATTGCCGTGA TTTGGCTGTT GGATACGCTG 601 CTGTTGTCGC GGATTCCTGT TTTGAGGAGA TGGTTTAAAT GA This corresponds to the amino acid sequence <SEQ ID 778; ORF 219>: m219.pep 1 MTARLRKCRG FWSLRLCLFQ GRXWAKTALT LTSRXHWKPS TALRGEIGFK 51 GRYQLΝLPKG EDGVWTLSQD SMSYDMISPF ADRTVHIDQY SGKILADIRF 101 DDYΝPFGKFM AASIALHMGT LGWWSVLAΝV LFCLAVIFIG ISGCVMWWKR 151 RPTGAVGIVP PAQKVKLPVW WMMALPLLAI ALLFPTSLLA IAVIWLLDTL 201 LLSRIPVLRR WFK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 219 shows 86.9% identity over a 213 aa overlap with a predicted ORF (ORF 219.ng) from N gonorrhoeae: m219/g219 10 20 30 40 50 60 m219.pep MTARLRKCRGFWSLRLCLFQGRXWAKTALTLTSRXHWKPSTALRGEIGFKGRYQLΝLPKG 1 1 1 1 1 1 1 1 i 1 1 1 1 1 I I I I I I h l l M I I : = M M I M M M M M g219 MTARLRKCRGFWSLCLCLSQGRLWVKTALTPPSPITLETVDRFAREIGFKGRYQLΝLPKG 10 20 30 40 50 60 70 80 90 100 110 120 m219. pep EDGVWTLSQDSMSYDMISPFADRTVHIDQYSGKILADIRFDDYΝPFGKFMAASIALHMGT g219 EDGVWTLSQDSMSYDMISPFADRTVHIDQYSGEILADIRFDDYΝPFGKFMAASIALHMGT 70 80 90 100 110 120 130 140 150 160 170 180 m219. pep LGWWSVLAlWLFCLAVIFIGISGCVMWWKRRPTGAVGIVPPAQKVKLPVWWMMALPLLAI M M I M M h M M M M M M M M M M h h M I M M I h l l M M I g219 LGWWSVLANWFCLAVIFIGISGCVMWWKRRPSGVAGIVPPAQKIKLPVWWAMALPLLLI 130 140 150 160 170 180 190 200 210 m219. pep ALLFPTSLLAIAVIWLLDTLLLSRIPVLRRWFKX M I M h l M M M M M M I M M M I h l M g219 ALLFPTALLAIAVIWLLDTLLLSRIPVLRKWFK 190 200 210 The following partial DNA sequence was identified in N meningitidis <SEQ TD 779>: a219.seq 1 ATGACGGCAA GGTTAAGGAA GTGCCGTGGG TTTTGGAGCT TACGCCTATG 51 CCTGTTTCAG GGACGACTGT GGGCAAAGAC GGTATTAACC CTGACGAGCC 101 GATGACATTG GAAACCGTCG ACCGTTTTGC GCGG . GAAAT CGGTTTCAAA 151 GGGCGTTATC AGCTGAATTT GCCCAAAGGC GAGGACGGCG TATGGACTTT 201 GTCGCAGGAT TCTATGAGTT ACGACATGAT CAGCCCGTTT GCTGACCGCA 251 CGGTGCATAT CGACCAGTAC AGCGGCAAGA TTCTTGCCGA CATCCGTTTT 301 GACGATTACA ACCCGTTCGG CAAATTTATG GCGGCAAGCA TTGCGCTGCA 351 TATGGGGACT TTGGGCTGGT GGAGCGTGTT GGCGAACGTT TTGTTCTGCC 401 TTGCCGTGAT TTTTATCGGC ATCAGCGGCT GCGTGATGTG GTGGAAACGC 451 CGTCCGTCCG GCGCGGTGGG CATGGTTCCG CCGGCGCAAA AAATCAAGCT 501 GCCCGTCTGG TGGGCAATGG CGGTGCCGCT GCTGCTGATT GCATTGCTTT 551 TCCCGACCGC GTTGCTTGCC ATTGCCGTGA TTTGGCTGTT GGATACGCTG 601 CTGTTGTCGC GGATTCCTGT TTTGAGGAGA TGGTTTAAAT GA This corresponds to the amino acid sequence <SEQ ID 780; ORF 219.a>: a219.pep 1 MTARLRKCRG FWSLRLCLFQ GRLWAKTVLT LTSR*HWKPS TVLRXEIGFK 51 GRYQLΝLPKG EDGVWTLSQD SMSYDMISPF ADRTVHIDQY SGKILADIRF 101 DDYΝPFGKFM AASIALHMGT LGWWSVLAΝV LFCLAVIFIG ISGCVMWWKR 151 RPSGAVGMVP PAQKIKLPVW WAMAVPLLLI ALLFPTALLA IAVIWLLDTL 201 LLSRIPVLRR WFK* m219/a219 94.8% identity in 213 aa overlap 10 20 30 40 50 60 m219. pep MTARLRKCRGFWSLRLCLFQGRXWAKTALTLTSRXHWKPSTALRGEIGFKGRYQLΝLPKG I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I: I I lllllllllllllll a219 MTARLRKCRGFWSLRLCLFQGRLWAKTVLTLTSRXHWKPSTVLRXEIGFKGRYQLΝLPKG 10 20 30 40 50 60 70 80 90 100 110 120 m219.pep EDGVWTLSQDSMSYDMISPFADRTVHIDQYSGKILADIRFDDYΝPFGKFMAASIALHMGT I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I II I I I I I I I I II II I I II I I I I I I I I I a219 EDGVWTLSQDSMSYDMISPFADRTVHIDQYSGKILADIRFDDYΝPFGKFMAASIALHMGT 70 80 90 100 110 120 130 140 150 160 170 180 m219. pep LGWWSVLAΝVLFCLAVIFIGISGCVMWWKRRPTGAVGIVPPAQKVKLPVWWMMALPLLAI I I I I I II I I I I I I I I I I I I I I II I I I I I I I I I : I I I I : I I I I I I : I I I I I I I I : I I I I a219 LGWWSVLAΝVLFCLAVIFIGISGCVMWWKRRPSGAVGMVPPAQKIKLPVWWAMAVPLLLI 130 140 150 160 170 180 190 200 210 m219.pep ALLFPTSLLAIAVIWLLDTLLLSRIPVLRRWFKX I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I a219 ALLFPTALLAIAVIWLLDTLLLSRIPVLRRWFKX 190 200 210 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 781>: g221.seq 1 atgcacgacc acggcgccat ggatcgccgc ctccccgctt tcggaagtct 51 gatgcggcga gccgtaaatc adatcgacgc tgacggattt gaaccctgcc 101 tcacgggcgg catcgatgac ttctttggtt tcttcgtagc tttggatgcg 151 gttgactgcc gcctgcactt tggggtcgaa atcctgaatg ccgacgctca 201 tgcggttgaa gccgagtctg ccgagcatga ggacggtgtc gcggctgact 251 ttgcgcgggt cgatttcgat ggaatattcg ccggacggta tcagttcgaa 301 atgtttgcgg atcatgcgga agacacgttc gatctgttcg tcgctcaaaa 351 aggtcggcgt gccgccgccg aagtgcagtt gggcaagctg gtgccgtccg 401 ttcagatgtg gagcgagcag ttccatttct ttttcaagat attcgatgta 451 ggtatcggcg cggcttttgt ctttggtgat gattttgttg cagccgcagt 501 agtagcagat ggtgttgcaa aacggaatgt gaatgtaaag ggaaagcggt 551 ttgtttaa This corresponds to the amino acid sequence <SEQ ID 782; ORF 221.ng>: g221.pep 1 MHDHGAMDRR LPAFGSLMRR AVΝXIDADGF EPCLTGGIDD FFGFFVALDA 51 VDCRLHFGVE ILΝADAHAVE AESAEHEDGV AADFARVDFD GIFAGRYQFE 101 MFADHAEDTF DLFVAQKGRR AAAEVQLGKL VPSVQMWSEQ FHFFFKIFDV 151 GIGAAFVFGD DFVAAAWAD GVAKRΝVΝVK GKRFV* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 783>: m221. seq 1 ATGGyGGTTT TGATGc cmg AAGTCTGGTG CGGCAGGCCG TAAATCAAAT 51 CGACGCTGAC GGATTTGAAC CCCGCTTCGC GCGCCGCATC GATGACTTCT 101 TTGGTTTCTT CGTAACTTTG GATGCGGTTG ACCGCCGCCT GCACTTTGGG 151 GTCGAAATCC TGAATGCCGA TGCTCATGCG GTTGAAGCCG AGTCTGCCGA 201 GCATGAGGAC GGTGTCGCGG CTGACTTTGC GCGGGTCGAT TTCGATGGAG 251 TATTCGCCGG TGGGGATTAA CTCGAAATGT TTGCGTATCA TGCGGAAGAC 301 ACGTTCGATC TGTTCGTCGC TCAAAAAGGt GCGTGCcCCG CCGAAGTGCA 351 GTTGGGCAAG CTGGTGCCGT CCGTTCAGAT GTGGAGCGAG CAGTTCCATT 401 TCTTTTTCAA GATATTCGAT GTAGGCATCG GCGCGGCTTT TGTCTTTGGT 451 GATGATTTTG TTGCAGCCGC AGTAGTAGCA GATGGTGTTG CAGAACGGAA 501 TGTGAATGTA AAGGGAAAGC GGTTTGTTTA A This corresponds to the amino acid sequence <SEQ ID 784; ORF 221>: m221.pep 1 MXVLMXRSLV RQAVΝQIDAD GFEPRFARRI DDFFGFFVTL DAVDRRLHFG 51 VEILΝADAHA VEAESAEHED GVAADFARVD FDGVFAGGDX LEMFAYHAED 101 TFDLFVAQKG ACPAEVQLGK LVPSVQMWSE QFHFFFKIFD VGIGAAFVFG 151 DDFVAAAWA DGVAERΝVΝV KGKRFV* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 221 shows 87.6% identity over a 170 aa overlap with a predicted ORF (ORF 22 l.ng) from N gonorrhoeae: m221/g221 10 20 30 40 50 m2 1. pep MXVLMXRSLVRQAVΝQIDADGFEPRFARRIDDFFGFFVTLDAVDRRLHFGVE I h h l l l I I I I M I I - 1 1 1 1 1 1 1 1 I ! 1 1 1 1 1 i 1 1 1 E g221 MHDHGAMDRRLPAFGSLMRRAVΝXIDADGFEPCLTGGIDDFFGFFVALDAVDCRLHFGVE 10 20 30 40 50 60 60 70 80 90 100 110 m221. pep ILΝADAHAVEAESAEHEDGVAADFARVDFDGVFAGGDXLEMFAYHAEDTFDLFVAQKGA- M M M M M M M M M M M M I M M I h l l l M i l l M M M M M M M g221 ILΝADAHAVEAESAEHEDGVAADFARVDFDGIFAGRYQFEMFADHAEDTFDLFVAQKGRR 70 80 90 100 110 120 120 130 140 150 160 170 m221.pep CPAEVQLGKLVPSVQMWSEQFHFFFKIFDVGIGAAFVFGDDFVAAAWADGVAERNVNVK MMIMMMMMMMMMMMMMMMMMMMMMMIhMMM g221 AAAEVQLGKLVPSVQMWSEQFHFFFKIFDVGIGAAFVFGDDFVAAAVVADGVAKRNVNVK 130 140 150 160 170 180 m221.pep GKRFVX g221 GKRFVX The following partial DNA sequence was identified in N meningitidis <SEQ ED 785>: a221. seq 1 ATGGTGGTTT TGATGCTCCG AAGTCTGGTG CGGCAGGCCG TAAATCAAAT 51 CGACGCTGAC GGATTTGAAC CCCGCTTCGC GCGCCGCATC GATGACTTCT 101 TTGGTTTCTT CGTAACTTTG GATGCGGTTG ACCGCCGCCT GCACTTTGGG 151 GTCGAAATCC TGAATGCCGA TGCTCATGCG GTTGAAGCCG AGTCTGCCGA 201 GCATGAGGAC GGTGTCGCGG CTGACTTTGC GCGGGTCGAT TTCGATGGAG 251 TATTCGCCGG TGGGGATTAA CTCGAAATGT TTGCGTATCA TGCGGAAGAC 301 ACGTTCGATT TGGTCGTCGC TCAAAAAGGT CGGCGTGCCG CCGCCGAAGT 351 GCAGTTGGGC AAGCTGGTGC CGTCCGTTCA GATGTGGAGC GAGCAGTTCC 401 ATTTCTTTTT CAAGAAATTC GATGTAGGCA TCGGCGCGGC TTTTGTCTTT 451 GGTGATGATT TTGTTGCAGC CGCAGTAGTA GCAGATGGTG TTGCAGAACG 501 GAATGTGAAT GTAAAGGGAA AGCGGTTTGT TTAA This corresponds to the amino acid sequence <SEQ ID 786; ORF 221.a>: a221.pep 1 MVVLMLRSLV RQAVΝQIDAD GFEPRFARRI DDFFGFFVTL DAVDRRLHFG 51 VEILΝADAHA VEAESAEHED GVAADFARVD FDGVFAGGD* LEMFAYHAED 101 TFDLWAQKG RRAAAEVQLG KLVPSVQMWS EQFHFFFKKF DVGIGAAFVF 151 GDDFVAAAW ADGVAERΝVΝ VKGKRFV* m221/a221 95.5% identity in 177 aa overlap 10 20 30 40 50 60 m221. pep MXVLMXRSLVRQAVΝQIDADGFEPRFARRIDDFFGFFVTLDAVDRRLHFGVEILΝADAHA I III I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a221 MVVLMLRSLVRQAVΝQIDADGFEPRFARRIDDFFGFFVTLDAVDRRLHFGVEILΝADAHA 10 20 30 40 50 60 70 80 90 100 110 119 m221. pep VEAESAEHEDGVAADFARVDFDGVFAGGDXLEMFAYHAEDTFDLFVAQKGA-CPAEVQLG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I llllll a221 VEAESAEHEDGVAADFARVDFDGVFAGGDXLEMFAYHAEDTFDLWAQKGRRAAAEVQLG 70 80 90 100 110 120 120 130 140 150 160 170 m221. pep KLVPSVQMWSEQFHFFFKIFDVGIGAAFVFGDDFVAAAWADGVAERΝVΝVKGKRFVX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a221 KLVPSVQMWSEQFHFFFKKFDVGIGAAFVFGDDFVAAAWADGVAERΝVΝVKGKRFVX 130 140 150 160 170 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 787>: g223.seq 1 atggaattca ggcaccaggt agtggtagtt ggtgtcgaac catttggtca 51 tttcgatggc gaattggtct ttgttgccgc gcgccagttg gaagaattgt 101 tccaaaggca ggttttggct atcgaagccg aaacgggcgg gaatcgcgcc 151 cgtggatact tgcaggtcga ggatgtgatg gtagaaagtg aaatcacgta 201 cagcaacgta atcagcgtta ggagcagctt ggtgtttcca gtttttctcg 251 cgcaggtctt tggcaacgtc gagcagctct tgttcactga tctctttgcg 301 ccagtatttt tcttgggcga atttcaattc acggaaggcg ccgacacgcg 351 ggaagcctga This corresponds to the amino acid sequence <SEQ ID 788; ORF 223.ng>: g223. pep . . 1 MEFRHQWW GVEPFGHFDG ELVFVAARQL EELFQRQVLA IEAETGGNRA 51 RGYLQVEDVM VESEITYSNV ISVRSSLVFP VFLAQVFGNV EQLLFTDLFA 101 PVFFLGEFQF TEGADTREA* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 789>: m223. seq 1 GTGGAATTCA GGCACCAAGT AGTGGTAGTT GGTGTCGAAC CATTTGGTCA 51 TTTCGATAGC GAATTGGTCT TTGTTACCGC GCGCCAGTTG GAAGAATTGT 101 TCCAAAGACA GGTTTTGGCT GTCGAAGCCG AAGCGGGCGG GAATCGCGCC 151 GGTGGCGACT TGCAGGTCGA GGATGTGGTC GTAGAAAGTG AAATCsCTAC 201 GGCAACGAAA TCGGCGTTGG CAGCGACCTG GTGTTTCCAG TTTTTCTCGC . 251 GCAAGTCTTT AGCAACAGCC AGCAATTCTT GCTCGCTGAT TTCTTTGCGC 301 CAGTATTTTT CTTGTGCGAA TTTCAATTCG CGGAAGGCGC CGACACGCGG 351 GAAGCCTGA This corresponds to the amino acid sequence <SEQ TD 790; ORF 223>: m223.pep 1 VEFRHQWW GVEPFGHFDS ELVFVTARQL EELFQRQVLA VEAEAGGΝRA 51 GGDLQVEDW VESEIXYGΝE IGVGSDLVFP VFLAQVFSΝS QQFLLADFFA 101 PVFFLCEFQF AEGADTREA* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 223 shows 80.7% identity over a 119 aa overlap with a predicted ORF (ORF 223. ng) from N gonorrhoeae: m223/g223 10 20 30 40 50 60 m223.pep VEFRHQWWGVEPFGHFDSELVFVTARQLEELFQRQVLAVEAEAGGΝRAGGDLQVEDW : I I I I I I I I I I I I I I I I I I : I I I I I : I I I I I I I I I I I I I I : I I I : I I II I I llllll: g223 MEFRHQWWGVEPFGHFDGELVFVAARQLEELFQRQVLAIEAETGGΝRARGYLQVEDVM 10 20 30 40 50 60 70 80 90 100 110 120 m223.pep VESEIXYGΝEIGVGΞDLVFPVFLAQVFSΝSQQFLLADFFAPVFFLCEFQFAEGADTREAX I I I I I : I : I I : I I : I II I I I I I I I I : I : I : I : : I : I I I I I I I I I I I : I II I I I I I I g223 VESEITYSΝVISVRSSLVFPVFLAQVFGΝVEQLLFTDLFAPVFFLGEFQFTEGADTREAX 70 80 90 100 110 The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 791>: a223. seq 1 GTGGAATTCA GGCACCAAGT AGTGGTAGTT GGTGTCGAAC CATTTGGTCA 51 TTTCGATAGC GAATTGGTCT TTGTTACCGC GCGCCAGTTG GAAGAATTGT 101 TCCAAAGATA GGTTTTGGCT GTCGAAGCCG AAGCGGGCGG GAATCGCGCC 151 GGTGGCGACT TGCAGGTCGA GGATGTGGTC GTAGAAAGTG AAATCGCCTA 201 CGGCAACGTA ATCGGCGTTG GCAGCGGCCT GGTGTTTCCA GTTTTTCTCG 251 CGCAAGTCTT TAGCAACAGC CAGCAATTCT TGCTCGCTGA TTTCTTTGCG 301 CCAGTATTTT TCTTGTGCGA ATTTCAATTC GCGGAAGGCA CCGACACGCG 351 GGAAGCCTGA This corresponds to the amino acid sequence <SEQ ID 792; ORF 223. a>: a223.pep 1 VEFRHQWW GVEPFGHFDS ELVFVTARQL EELFQR*VLA VEAEAGGΝRA 51 GGDLQVEDW VESEIAYGΝV IGVGSGLVFP VFLAQVFSΝS QQFLLADFFA 101 PVFFLCEFQF AEGTDTREA* m223/a223 95.8% identity in 119 aa overlap 10 20 30 40 50 60 m223 . pep VEFRHQWWGVEPFGHFDSELVFVTARQLEELFQRQVLAVEAEAGGΝRAGGDLQVEDW I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a223 VEFRHQWWGVEPFGHFDSELVFVTARQLEELFQRXVLAVEAEAGGΝRAGGDLQVEDVV 10 20 30 40 50 60 70 80 90 100 110 120 m223 . pep VEΞEIXYGΝEIGVGSDLVFPVFLAQVFSΝSQQFLLADFFAPVFFLCEFQFAEGADTREAX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I 1 : 1 I I I I I a223 VESEIAYGΝVIGVGSGLVFPVFLAQVFSΝSQQFLLADFFAPVFFLCEFQFAEGTDTREAX 70 80 90 100 110 120 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 793>: g225.seq 1 atggattctt ttttcaaacc ggcagtttgg gcggttttgt ggctgatgtt 51 tgccgtccgc cccgcccttg ccgacgagtt gaccaacctg ctcagcagcc 101 gcgagcagat tctcagacag tttgccgaag acgaacagcc cgttttaccc 151 gtcaaccgag cccccgcccg gcgggcgggc aatgccgacg aactcatcgg 201 cggcgcgatg gggcttaacg aacagcccgt tgtacgcgtc aaccgagccn 251 ccgcccggcg ggcgggcaat gccgacaaac tcatcggcag cgcgatgcgg 301 cttttgggta ttgcctaccg ctacggcggc acatcggtgt ctaccggttt 351 tgactgcagc ggattcatgc agcacatctt caaacgcgcc atgggcatca 401 acctgccgcg cacgtcggcg gaacaggcgc ggatgggcgc acccgttgcc 451 cgaagcgaat tgcagcccgg ggatatggtg tttttccgca cgctcggcgg 501 cagccgcatt tcccatgtcg gactttatat cggcaacaac cgcttcatcc 551 acgcgccgcg cacggggaaa aatatcgaaa tcaccagcct gagccacaaa 601 tattggagcg gcaaatatgc gttcgcccgc cgggtcaaga aaaacgaccc 651 gtcacgcttt ctgaactga This corresponds to the amino acid sequence <SEQ ID 794; ORF 225. ng>: g2 5.pep 1 MDSFFKPAVW AVLWLMFAVR PALADELTΝL LSSREQILRQ FAEDEQPVLP 51 VΝRAPARRAG ΝADELIGGAM GLΝEQPWRV ΝRAXARRAGΝ ADKLIGSAMR 101 LLGIAYRYGG TSVSTGFDCS GFMQHIFKRA MGIΝLPRTSA EQARMGAPVA 151 RSELQPGDMV FFRTLGGSRI SHVGLYIGΝΝ RFIHAPRTGK ΝIEITSLSHK 201 YWSGKYAFAR RVKKΝDPSRF LΝ* The following partial DΝA sequence was identified in N meningitidis <SEQ ED 795>: m225.seq (partial) 1 .. TTTTCAAACC CGGCAGTTTG GGCGGTTTTG TGGCTGAwGT TTGCCGTCCG 51 CCCCGCCCTT GCCGACGAGT TGACCAACCT GCTCAGCAGC CGCGAGCAGA 101 TTCTCAGACA GTTTGCCGAA GACGAACAGC CCGTTTTACC CATCAACCGA 151 GCCCCCGCCC GGCGGGCGGG CAATGCCGAC GAACTCATCG GCAGCGCGAT 201 GGGGCTTAAC GAACAGCCCG TTTTACCCGT CAACCGAGTC CCCGCCCGGC 251 GGGCGGGCAA TGCCGACGAA CTCATCGGCA ACGCGATGGG GCTTAACGAA 301 CAGCCCGTTT TACCCGTCAA CCGAGCCCCC GCCCGGCGGG CGGGCAATGC 351 CGACGAACTC ATCGGCAACG CGATGGGACT TTTGGGTATT GCCTACCGCT 401 ACGGCGGCAC ATCGGTTTCT ACCGGTTTTG ACTGCAGCGG CTTCATGCAG 451 CACATCTTCA AACGCGCCAT GGGCATCAAC CTGCCGCGCA CGTCGGCAGA 501 ACAGGCACGG ATGGGTACGC CGGTTGCCCG AAGCGAATTG CAGCCCGGAG 551 ATATGGTGTT TTTCCGCACG CTCGGCGGCA GCCGCATTTC CCATGTCGGA 601 CTTTATATCG GCAACAACCG CTTCATCCAC GCGCCGCGCA CGGGGAAAAA 651 TATCGAAATC ACCAGCCTGA GCCACAAATA TTGGAGCGGC AAATACGCGT 701 TCGCCCGCCG GGTCAAGAAA AACGACCCGT CCCGCTTTCT GAACTGA This corresponds to the amino acid sequence <SEQ ID 796; ORF 225>: m225.pep (partial) 1 ..FSΝPAVWAVL WLXFAVRPAL ADELTΝLLSS REQILRQFAE DEQPVLPIΝR 51 APARRAGΝAD ELIGSAMGLΝ EQPVLPVΝRV PARRAGΝADE LIGΝAMGLΝE 101 QPVLPVΝRAP ARRAGΝADEL IGΝAMGLLGI AYRYGGTSVS TGFDCSGFMQ 151 HIFKRAMGIΝ LPRTSAEQAR MGTPVARSEL QPGDMVFFRT LGGSRISHVG 201 LYIGΝΝRFIH APRTGKΝIEI TSLSHKYWSG KYAFARRVKK ΝDPSRFLΝ* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 225 shows 83.5% identity over a 248 aa overlap with a predicted ORF (ORF 225.ng) from N gonorrhoeae: m225/g225 10 20 30 40 50 m225.pep FSΝPAVWAVLWLXFAVRPALADELTΝLLSSREQILRQFAEDEQPVLPIΝRAPARRAG I M M M I I I I M M M M I M M M M M M M M M M M M h l M M M M g225 MDSFFKPAVWAVLWLMFAVRPALADELTΝLLSSREQILRQFAEDEQPVLPVΝRAPARRAG 10 20 30 40 50 60 60 70 80 90 100 110 m225.pep NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNA MIIMMIh MM Mlllll g225 NADELIG GAMGLNEQPWRVNRAXARRAGNA 70 80 90 120 130 140 150 160 170 m225.pep DELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVAR hllhll Mill Mill I II Mill 11 II III IIIIIMI III II Ihl III g225 DKLIGSAMRLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGAPVAR 100 110 120 130 140 150 180 190 200 210 220 230 m225.pep SELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR I MMMMMMMMMMMM 11 Mill II Mill II III I II g225 SELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR 160 170 180 190 200 210 240 249 m225. pep VKKNDPSRFLNX IIIMMMM g225 VKKNDPSRFLN 220 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 797>: a225. seq 1 ATGGATTCTT TTTTCAAACC GGCAGTTTGG GCGGTTTTGT GGCTGATGTT 51 TGCCGTCCGC CCCGCCCTTG CCGACGAGTT GACCAACCTG CTCAGCAGCC 101 GCGAGCAGAT TCTCAGACAG TTTGCCGAAG ACGAACAGCC CGTTTTACCC 151 ATCAACCGAΝ CCCCCGCCCG GCGGGCGGGC AATGCCGACG AACTCATCGG 201 CAGCGCGATG GGGCTTAACG AACAGCCCGT TTTACCCGTC AACCGAΝTCC 251 CCGCCCGGCG GGCGGGCAAT GCCGACΝAAC TCATCGGCAA CGCGATGGGG 301 CTTAACGAAC AGCCCGTTTT ACCCGTCAAC CGAGTCCCCG CCCGGCGGGC 351 GGGCAATGCC GACGAACTCA TCGGCAACGC GATGGGGCTT AACGAACAGC 401 CCGTTTTACC CGTCAACCGA GCCCCCGCCC GGCGGGCGGG CAATGCCGAC 451 GAACTCATCG GCAACGCGAT GGGACTTTTG GGTATTGCCT ACCGCTACGG 501 CGGCACATCG ATTTCTACCG GTTTTGACTG CAGCGGCTTC ATGCAGCACA 551 TCTTCAAACG CGCCATGGGC ATCAACCTGC CGCGCACGTC GGCAGAACAG 601 GCGCGGATGG GTACGCCGGT TGCCCGAAGC GAATTGCAGC CCGGGGATAT 651 GGTGTΝTTTC CGCACGCTCG GCGGCAGCCG CATTTCCCAT GTCGGACTTT 701 ATATCGGCAA CAACCGCTTC ATCCACGCGC CGCGCACGGG GAAAAATATC 751 GAAATCACCA GCCTGAGCCA CAAATATTGG AGCGGCAAAT ACGCGTTCGC 801 CCGCCGGGTC AAGAAAAACG ACCCGTCCCG CTTTCTGAAC TGA This corresponds to the amino acid sequence <SEQ ID 798; ORF 225. a>: a225.pep 1 MDSFFKPAVW AVLWLMFAVR PALADELTΝL LSSREQILRQ FAEDEQPVLP 51 IΝRXPARRAG ΝADELIGSAM GLΝEQPVLPV ΝRXPARRAGΝ ADXLIGΝAMG 101 LΝEQPVLPVΝ RVPARRAGΝA DELIGΝAMGL ΝEQPVLPVΝR APARRAGΝAD 151 ELIGΝAMGLL GIAYRYGGTS ISTGFDCSGF MQHIFKRAMG IΝLPRTSAEQ 201 ARMGTPVARS ELQPGDMVXF RTLGGSRISH VGLYIGΝΝRF IHAPRTGKΝI 251 EITSLSHKYW SGKYAFARRV KKΝDPSRFLΝ * m225/a225 87.4% identity in 277 aa overlap 10 20 30 40 50 m225. pep FSΝPAVWAVLWLXFAVRPALADELTΝLLSSREQILRQFAEDEQPVLPIΝRAPARRAG I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I II I a225 MDSFFKPAVWAVLWLMFAVRPALADELTΝLLSSREQILRQFAEDEQPVLPIΝRXPARRAG 10 20 30 40 50 60 60 70 79 80 m225. pep ΝADELIGSAMGLΝEQPVLPVΝR VPARRAGΝA I I I I I I I I I I I I I I I I I I I I I I lllllllll a225 NADELIGSAMGLNEQPVLPVNRXPARRAGNADXLIGNAMGLNEQPVLPVNRVPARRAGNA 70 80 90 100 110 120 90 100 110 120 130 140 m225. pep DELIGNAMGLNEQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSVSTGFDCSGF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I a225 DELIGNAMGLNEQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSISTGFDCSGF 130 140 150 160 170 180 150 160 170 180 190 200 m225.pep MQHIFKRAMGINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a225 MQHIFKRAMGINLPRTSAEQARMGTPVARSELQPGDMVXFRTLGGSRISHVGLYIGNNRF 190 200 210 220 230 240 210 220 230 240 249 m225. pep IHAPRTGKNIEITSLSHKYWSGKYAFARRVKKNDPSRFLNX I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I a225 IHAPRTGKNIEITSLSHKYWSGKYAFARRVKKNDPSRFLNX 250 260 270 280 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 799>: g225-l.seq 1 atggattctt ttttcaaacc ggcagtttgg gcggttttgt ggctgatgtt 51 tgccgtccgc cccgcccttg ccgacgagtt gaccaacctg ctcagcagcc 101 gcgagcagat tctcagacag tttgccgaag acgaacagcc cgttttaccc 151 gtcaaccgag cccccgcccg gcgggcgggc aatgccgacg aactcatcgg 201 cggcgcgatg gggcttaacg aacagcccgt tgtacgcgtc aaccgagccn 251 ccgcccggcg ggcgggcaat gccgacaaac tcatcggcag cgcgatgcgg 301 cttttgggta ttgcctaccg ctacggcggc acatcggtgt ctaccggttt 351 tgactgcagc ggattcatgc agcacatctt caaacgcgcc atgggcatca 401 acctgccgcg cacgtcggcg gaacaggcgc ggatgggcgc acccgttgcc 451 cgaagcgaat tgcagcccgg ggatatggtg tttttccgca cgctcggcgg 501 cagccgcatt tcccatgtcg gactttatat cggcaacaac cgcttcatcc 551 acgcgccgcg cacggggaaa aatatcgaaa tcaccagcct gagccacaaa 601 tattggagcg gcaaatatgc gttcgcccgc cgggtcaaga aaaacgaccc 651 gtcacgcttt ctgaactga This corresponds to the amino acid sequence <SEQ ID 800; ORF 225-l.ng>: g225-l.pep 1 MDSFFKPAVW AVLWLMFAVR PALADELTΝL LSSREQILRQ FAEDEQPVLP 51 VΝRAPARRAG ΝADELIGGAM GLΝEQP RV ΝRAXARRAGΝ ADKLIGSAMR 101 LLGIAYRYGG TSVSTGFDCS GFMQHIFKRA MGIΝLPRTSA EQARMGAPVA 151 RSELQPGDMV FFRTLGGSRI SHVGLYIGΝΝ RFIHAPRTGK ΝIEITSLSHK 201 YWSGKYAFAR RVKKΝDPSRF LΝ* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 801>: m225-l.seq 1 ATGGATTCTT TTTTCAAACC GGCAGTTTGG GCGGTTTTGT GGCTGATGTT 51 TGCCGTCCGC CCCGCCCTTG CCGACGAGTT GACCAACyTG CTCAGCAGCC 101 GCGAGCAGAT TCTCAGACAG TTTGCCGAAG ACGAACAGCC CGTTTTACCC 151 ATCAACCGAG CCCCCGCCCG GCGGGCGGGC AATGCCGACG AACTCATCGG 201 CAGCGCGATG GGGCTTAACG AACAGCCCGT TTTACCCGTC AACCGAGTCC 251 CCGCCCGGCG GGCGGGCAAT GCCGACGAAC TCATCGGCAA CGCGATGGGG 301 CTTAACGAAC AGCCCGTTTT ACCCGTCAAC CGAGCCCCCG CCCGGCGGGC 351 GGGCAATGCC GACGAACTCA TCGGCAACGC GATGGGACTT TTGGGTATTG 401 CCTACCGCTA CGGCGGCACA TCGGTTTCTA CCGGTTTTGA CTGCAGCGGC 451 TTCATGCAGC ACATCTTCAA ACGCGCCATG GGCATCAACC TGCCGCGCAC 501 GTCGGCAGAA CAGGCACGGA TGGGTACGCC GGTTGCCCGA AGCGAATTGC 551 AGCCCGGAGA TATGGTGTTT TTCCGCACGC TCGGCGGCAG CCGCATTTCC 601 CATGTCGGAC TTTATATCGG CAACAACCGC TTCATCCACG CGCCGCGCAC 651 GGGGAAAAAT ATCGAAATCA CCAGCCTGAG CCACAAATAT TGGAGCGGCA 701 AATACGCGTT CGCCCGCCGG GTCAAGAAAA ACGACCCGTC CCGCTTTCTG 751 AACTGA This corresponds to the amino acid sequence <SEQ ID 802; ORF 217>: m225-l.pep 1 MDSFFKPAVW AVLWLMFAVR PALADELTNL LSSREQILRQ FAEDEQPVLP 51 INRAPARRAG NADELIGSAM GLNEQPVLPV NRVPARRAGN ADELIGNAMG 101 LNEQPVLPVN RAPARRAGNA DELIGNAMGL LGIAYRYGGT SVSTGFDCSG 151 FMQHIFKRAM GINLPRTSAE QARMGTPVAR SELQPGDMVF FRTLGGSRIS 201 HVGLYIGNNR FIHAPRTGKN IEITSLSHKY WSGKYAFARR VKKNDPSRFL 251 N* 225-l/g225-l 84.9% identity in 251 aa overlap 10 20 30 40 50 60 m225-l .pep MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG g225-1 MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPVNRAPARRAG 10 20 30 40 50 60 70 80 90 100 110 120 m225-l .pep NADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLNEQPVLPVNRAPARRAGNA M i l M M g225-1 NADE LIGGAMGLNEQPWRVNRAXARRAGNA 70 80 90 130 140 150 160 170 180 m225-l.pep DELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGTPVAR h l l h l l g225-l DKLIGSAMRLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAMGINLPRTSAEQARMGAPVAR 100 110 120 130 140 150 190 200 210 220 230 240 m225-l.pep SELQPGDMVFFRTLGGΞRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR g225-1 SELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTGKNIEITSLSHKYWSGKYAFARR 160 170 180 190 200 210 250 m225-l.pep VKKNDPSRFLNX g225-l VKKNDPSRFLNX 220 The following partial DNA sequence was identified in N meningitidis <SEQ ID 803>: a225-l.seq 1 ATGGATTCTT TTTTCAAACC GGCAGTTTGG GCGGTTTTGT GGCTGATGTT 51 TGCCGTCCGC CCCGCCCTTG CCGACGAGTT GACCAACCTG CTCAGCAGCC 101 GCGAGCAGAT TCTCAGACAG TTTGCCGAAG ACGAACAGCC CGTTTTACCC 151 ATCAACCGAΝ CCCCCGCCCG GCGGGCGGGC AATGCCGACG AACTCATCGG 201 CAGCGCGATG GGGCTTAACG AACAGCCCGT TTTACCCGTC AACCGAΝTCC 251 CCGCCCGGCG GGCGGGCAAT GCCGACΝAAC TCATCGGCAA CGCGATGGGG 301 CTTAACGAAC AGCCCGTTTT ACCCGTCAAC CGAGTCCCCG CCCGGCGGGC 351 GGGCAATGCC GACGAACTCA TCGGCAACGC GATGGGGCTT AACGAACAGC 401 CCGTTTTACC CGTCAACCGA GCCCCCGCCC GGCGGGCGGG CAATGCCGAC 451 GAACTCATCG GCAACGCGAT GGGACTTTTG GGTATTGCCT ACCGCTACGG 501 CGGCACATCG ATTTCTACCG GTTTTGACTG CAGCGGCTTC ATGCAGCACA 551 TCTTCAAACG CGCCATGGGC ATCAACCTGC CGCGCACGTC GGCAGAACAG 601 GCGCGGATGG GTACGCCGGT TGCCCGAAGC GAATTGCAGC CCGGGGATAT 651 GGTGTΝTTTC CGCACGCTCG GCGGCAGCCG CATTTCCCAT GTCGGACTTT 701 ATATCGGCAA CAACCGCTTC ATCCACGCGC CGCGCACGGG GAAAAATATC 751 GAAATCACCA GCCTGAGCCA CAAATATTGG AGCGGCAAAT ACGCGTTCGC 801 CCGCCGGGTC AAGAAAAACG ACCCGTCCCG CTTTCTGAAC TGA This corresponds to the amino acid sequence <SEQ ID 804; ORF 225-1. a>: a225-l.pep 1 MDSFFKPAVW AVLWLMFAVR PALADELTΝL LSSREQILRQ FAEDEQPVLP 51 IΝRXPARRAG ΝADELIGSAM GLΝEQPVLPV ΝRXPARRAGΝ ADXLIGΝAMG 101 LΝEQPVLPVΝ RVPARRAGΝA DELIGΝAMGL ΝEQPVLPVΝR APARRAGΝAD 151 ELIGΝAMGLL GIAYRYGGTS ISTGFDCSGF MQHIFKRAMG IΝLPRTΞAEQ 201 ARMGTPVARS ELQPGDMVXF RTLGGSRISH VGLYIGΝΝRF IHAPRTGKΝI 251 EITSLSHKYW SGKYAFARRV KKΝDPΞRFLΝ * a225-l/m225-l 88.6% identity in 280 aa overlap 10 20 30 40 50 60 a225-l .pep MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRXPARRAG m225-1 MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAEDEQPVLPINRAPARRAG 10 20 30 40 50 60 70 80 90 100 110 120 a225-l .pep NADELIGSAMGLNEQPVLPVNRXPARRAGNADXLIGNAMGLNEQPVLPVNRVPARRAGNA I I M I N I M m225 -l NADELIGSAMGLNEQP VLPVNRVPARRAGNA 70 80 90 130 140 150 160 170 180 a225-l .pep DELIGNAMGLNEQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSISTGFDCSGF N N I M i M M . M i l l l M l I I I I M M I M N i I i i m225-l DELIGNAMGLNEQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSVSTGFDCSGF 100 110 120 130 140 150 190 200 210 220 230 240 a225-l .pep MQHIFKRAMGINLPRTSAEQARMGTPVARSELQPGDMVXFRTLGGSRISHVGLYIGNNRF m225-1 MQHIFKRAMGINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRF 160 170 180 190 200 210 250 260 270 280 a225-l .pep IHAPRTGKNIEITSLSHKYWSGKYAFARRVKKNDPΞRFLNX m225-l IHAPRTGKNIEITSLSHKYWSGKYAFARRVKKNDPSRFLNX 220 230 240 250 The following partial DNA sequence was identified in N gonorrhoeae <SEQ LD 805>: g226. seq 1 ATGAGCGAAA TCCTCAGGCA GCCCAGCGTT CTGCTTTTCC TCACGCTTGC 51 CGTGTACGCG CTTGCGATTA TCGTGCGCAC GCGCACGGGC AATATCTTCT 101 GCAACCCCGT ACTCGTCAGC ACTATCGTGC TGATTGCCTA CCTGAAAATC 151 CTCGGTATCG ATTATGCGGT GTACCACAAC GCCGCGCAAT TCATTGATTT 201 TCGGCTGAAA cccGccgtCG TCGTGCTTGC CGTGCCGCTC TACCAAAACC 251 GCCGTAAAAT CTTCAACCAG TGGCTGCCCG TCATCGTTTC GCAGCTTGCG 301 GGCAGCGTTA cggGCATTGT tacggggATG TATTTTgccg cttggctcgg 351 gccggatacc caattctcct tcccgcctcg tcttcaatat ctgttattta 401 caccctctgg aatcccaatt cacaccctgt atgcgcgggt tctcccgcca 451 tttctgttgc ctccgcctct cctgccgcgc ctcggcccgc atacattgcg 501 ccggttcaca atacttccaa aaaaactacg gccgtttaag cccctcctcc 551 cagttgtggt cctttctcct Ccgggcctcg cccctcccct cttataa This corresponds to the amino acid sequence <SEQ ID 806; ORF 226.ng>: g226.pep 1 MSEILRQPSV LLFLTLAVYA LAIIVRTRTG ΝIFCΝPVLVS TIVLIAYLKI 51 LGIDYAVYHΝ AAQFIDFRLK PAVWLAVPL YQΝRRKIFΝQ WLPVIVSQLA 101 GSVTGIVTGM YFAAWLGPDT QFSFPPRLQY LLFTPSGIPI HTLYARVLPP 151 FLLPPPLLPR LGPHTLRRFT ILPKKLRPFK PLLPVWLSP PGLAPPLL* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 807>: m226. seq 1 ATGAACGAAA TCCTCAGGCA GCCCAGCGTT CTGCTTTTCC TCACGCTTGC 51 CGTGTACGCG CTTGCGATTA TCGtGCGCAC GCGCACGGGC AATATCTTCT 101 GCAACCCCGT ACTCGTCAGC ACTATCGTGC TGATTGCCTA CCTGAAAATC 151 CTCGGTATCG ATTATGCGGT GTACCACAAC GCCGCGCAAT TCATTGATTT 201 TTGGCTGAAA CCCGCCGTCG TCGTGCTTGC CGTGCCGCTC TACCAAAACC 251 GCCGTAAAAT CTTCAACCAG TGGCTGCCCG TCATCGTTTC ACAGCTTGCG 301 GGCAGCGTTA CGGGCATTGT TACAGGGATG TATTTTGCCA AATGGCTGGG 351 CGCGGAACGC GAAGTCGTCC TCTCGCTCGC GTCCAAATCT GTTACCAACC 401 CCATCGCTAT TGAAATCACC CGCTCCATCG GCGGCATTCC CGCCATTACC 451 GCCGCCACCG TCATCATTGC CGGTCTGGTC GGACAGATTG CCGGTTACAA 501 AATGCTGAAG AACACGGTCG TCATGCCCTC GTCCGTGGGT ATGTCGCTCG 551 GCACGGCTTC GCACGCGATG GGGATTGCCG CCTCGCTCGA ACGCAGCCGC 601 CGTATGGCGG CATACGCGGG GCTGGGGCTG ACGTTCAACG GCGTACTGAC 651 CGCGCTGATT GCGCCGCTGC TCATCCCCGT TTTGGGATTT TGA This corresponds to the amino acid sequence <SEQ ID 808; ORF 226>: m226.pep 1 MNEILRQPSV LLFLTLAVYA LAIIVRTRTG NIFCNPVLVS TIVLIAYLKI 51 LGIDYAVYHN AAQFIDFWLK PAVWLAVPL YQNRRKIFNQ WLPVIVSQLA 101 GSVTGIVTGM YFAKWLGAER EWLSLASKS VTNPIAIEIT RSIGGIPAIT 151 AATVIIAGLV GQIAGYKMLK NTWMPSSVG MSLGTASHAM GIAASLERSR 201 RMAAYAGLGL TFNGVLTALI APLLIPVLGF * Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 226 shows 94.2% identity over a 121 aa overlap with a predicted ORF (ORF 226.ng) from N gonorrhoeae: m226/g226 10 20 30 40 50 60 m226.pep MΝEILRQPSVLLFLTLAVYALAIIVRTRTGΝIFCΝPVLVSTIVLIAYLKILGIDYAVYHΝ hllll II Mill 11 MM III III II III Mill III I III 11 III II II II g226 MSEILRQPSVLLFLTLAVYALAIIVRTRTGΝIFCΝPVLVSTIVLIAYLKILGIDYAVYHΝ 10 20 30 40 50 60 70 80 90 100 110 120 m226. pep AAQFIDFWLKPAVWLAVPLYQΝRRKIFΝQWLPVIVSQLAGSVTGIVTGMYFAKWLGAER l l l l l l l M M M M M M M M M M M M M M M M M M I M M M M I I I ■ g226 AAQFIDFRLKPAVWLAVPLYQΝRRKI FΝQWLPVIVSQLAGSVTGI VTGMYFAAWLGPDT 70 80 90 100 110 120 130 140 150 160 170 180 m226 . pep EWLSLASKSVTΝPIAIEITRSIGGIPAITAATVIIAGLVGQIAGYKMLKΝTWMPSSVG g226 QFSFPPRLQYLLFTPSGIPIHTLYARVLPPFLLPPPLLPRLGPHTLRRFTILPKKLRPFK 130 140 150 160 170 180 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 809>: a226. seq 1 ATGAACGAAA TCCTCAGGCA GCCGAGCATC CTGCTTTTCC TCACGCTTGC 51 CGTGTACGCG CTTGCGATTA TCGTGCGCAC GCGCACGGGT AATATCTTCT 101 GCAACCCCGT ACTCGTCAGC ACTATCGTGC TGATTGCCTA CCTGAAAATC 151 CTCGGTATCG ATTATGCGGT GTACCACAAC GCCGCGCAGT TTATCGATTT 201 CTGGCTCAAG CCCGCCGTCG TCGTGCTTGC CGTGCCGCTC TACCAAAACC 251 GCCGTAAAAT CTTCAACCAA TGGCTGCCCG TCATCGTTTC GCAGCTTGCG 301 GGCAGCGTTA CGGGCATTGT TACGGGGATG TATTTTGCCA AATGGCTGGG 351 CGCGGAACGC GAAGTCGTCC TCTCGCTCGC GTCCAAATCT GTTACCAATC 401 CTATCGCCAT CGAAATCACC CGCTCCATCG GCGGCATTCC CGCCATTACC 451 GCCGCCACCG TCATCATTGC CGGCCTGGTC GGACAGATTG CCGGTTACAA 501 AATGTTGAAA AACACGGTCG TTATGCCCTC ATCTGTCGGA ATGTCGCTCG 551 GCACGGCTTC GCACGCGATG GGCATTGCCG CCTCGCTCGA ACGCAGCCGC 601 CGCATGGCGG CATACGCGGG GCTGGGGCTG ACGTTCAACG GCGTACTGAC 651 CGCGCTGATT GCGCCGCTGC TTATCCCCGT TTTGGGATTT TGA This corresponds to the amino acid sequence <SEQ ID 810; ORF 226. a>: a226.pep 1 MΝEILRQPSI LLFLTLAVYA LAIIVRTRTG ΝIFCΝPVLVS TIVLIAYLKI 51 LGIDYAVYHN AAQFIDFWLK PAVWLAVPL YQNRRKIFNQ WLPVIVSQLA 101 GSVTGIVTGM YFAKWLGAER EWLSLASKS VTNPIAIEIT RSIGGIPAIT 151 AATVIIAGLV GQIAGYKMLK NTWMPSSVG MSLGTASHAM GIAASLERSR 201 RMAAYAGLGL TFNGVLTALI APLLIPVLGF * m226/a226 99.6% identity in 230 aa overlap 10 20 30 40 50 60 m226. pep MNEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVLIAYLKILGIDYAVYHN I I I I I I I I I: I I II I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I a226 MNEILRQPSILLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVLIAYLKILGIDYAVYHN 10 20 30 40 50 60 70 80 90 100 110 120 m226. pep AAQFIDFWLKPAVWLAVPLYQNRRKIFNQWLPVIVSQLAGSVTGIVTGMYFAKWLGAER I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I II I I I II I I I I a226 AAQFIDFWLKPAVWLAVPLYQNRRKIFNQWLPVIVSQLAGSVTGIVTGMYFAKWLGAER 70 80 90 100 110 120 130 140 150 160 170 180 m226.pep EWLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLVGQIAGYKMLKNTWMPSSVG I I I I I I I I I I II I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a226 EVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLVGQIAGYKMLKNTWMPSSVG 130 140 150 160 170 180 190 200 210 220 230 m226. pep MSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTALIAPLLIPVLGFX I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I a226 MSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTALIAPLLIPVLGFX 190 200 210 220 230 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 811>: g227.seq 1 atgaacatca tccgcgcgct cctcatcatc ctcggctgcc tcgccgccgg 51 cgaaaccgcc gttttcctag caggcatcaa actgcccggc agcatcgtcg 101 gcatgggcgt gctgtttgcg cttttgcagg cgggttggct caaaacgtct 151 tggctgcaac agcttaccga cgcgctgatg gcaaacctga cgctgttcct 201 cgtgccgccc tgcgtggcgg tcatcagcta tttggatttg attgccgacg 251 attggttttc gatactggtt tccgcctccg ccagcacttt gtgcgtactg 301 ctggttacgg gcaaggttca ccgctggata cggagcatta tctga This corresponds to the amino acid sequence <SEQ ID 812; ORF 227. ng>: g227.pep 1 MΝIIRALLII LGCLAAGETA VFLAGIKLPG SIVGMGVLFA LLQAGWLKTS 51 WLQQLTDALM AΝLTLFLVPP CVAVISYLDL IADDWFSILV SASASTLCVL 101 LVTGKVHRWI RSII* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 813>: m227.seq (partial) 1 ..ACGTCTT GC TGCAACAGCT TACCGACGCG CTGATGTCGA ACCTGACGCT 51 GTtCCTCGTG CCgCC.TGCG TGGCGGTCAT CAGCTATTTG GATTTGATTG 101 CCGACGATTG GTTTTCGATA CTGGTTTCCG CCTCCGCCAG cACTTTGTGC 151 GTACTGCTGG TTACGGGCAA AGTCCACCGG TGGATACGGG GTATTATCCG 201 ATGA This corresponds to the amino acid sequence <SEQ ID 814; ORF 227>: m227.pep (partial) 1 .. TSXLQQLTDA LMSΝLTLFLV PPCVAVISYL DLIADDWFSI LVSASASTLC 51 VLLVTGKVHR WIRGIIR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 227 shows 95.5% identity over a 66 aa overlap with a predicted ORF (ORF 227.ng) from N gonorrhoeae: m227/g227 10 20 30 m227.pep TSXLQQLTDALMSΝLTLFLVPPCVAVISYL II I I I I I I I I I: I I I II I I I I I I I I I I I I g227 TAVFLAGIKLPGSIVGMGVLFALLQAGWLKTSWLQQLTDALMAΝLTLFLVPPCVAVISYL 20 30 40 50 60 70 40 50 60 m227.pep DLIADDWFSILVSASASTLCVLLVTGKVHRWIRGIIRX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I g227 DLIADDWFSILVSASASTLCVLLVTGKVHRWIRSIIX 80 90 100 110 The following partial DNA sequence was identified inN meningitidis <SEQ ED 815>: a227.seq 1 ATGAACATCA TCCGCGCGCT CCTCATCATC CTCGGCTGCC TCGCCACCGG 51 CGAAACCGCC GTTTTCCTAG CAGGCATCAA ACTGCCCGGC AGCATCGTCG 101 GCATGGGCGT ACTGTTTGCG CTTTTGCAGG CGGGTTGGGT CAAAACGTCT 151 TGGCTGCAAC AGCTTACCGA CGCGCTGATG GCGAATCTGA CGTTGTTTCT 201 CGTGCCGCCC TGCGTGGCGG TCATCAGCTA TTTGGATTTG ATTGCCGACG 251 ATTGGTTTTC GATACTGGTT TCCGCCTCCG CCAGCACTTT GTGCGTACTG 301 CTGGTTACAG GCAAGGTTCA CCGCTGGATA CGGAGCATTA TCTGA This corresponds to the amino acid sequence <SEQ ED 816; ORF 227. a>: a227.pep 1 MΝIIRALLII LGCLATGETA VFLAGIKLPG SIVGMGVLFA LLQAGWVKTS 51 WLQQLTDALM AΝLTLFLVPP CVAVISYLDL IADDWFSILV SASASTLCVL 101 LVTGKVHRWI RSII* m227/a227 95.5% identity in 66 aa overlap 10 20 30 m227. pep TSXLQQLTDALMSΝLTLFLVPPCVAVISYL II I I I I I I I I I : I I I I I I I I I I I I I I I I I a227 TAVFLAGIKLPGSIVGMGVLFALLQAGWVKTSWLQQLTDALMAΝLTLFLVPPCVAVISYL 20 30 40 50 60 70 40 50 60 m227.pep DLIADDWFSILVSASASTLCVLLVTGKVHRWIRGIIRX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I a227 DLIADDWFSILVSASASTLCVLLVTGKVHRWIRSIIX 80 90 100 110 The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 817>: m228. seq 1 ATGAAAAAAT TATTGATTGC CGCAATGATG GCGGCTGCCT TGGCAGCTTG 51 TTCGCAAGAA GCCAAACAGG AGGTTAAGGA AGCGGTTCAA GCCGTTGAGT 101 CCGATGTTAA AGACACTGCG GCTTCTGCCG CCGAGTCTGC CGCTTCTGCC 151 GTCGAAGAAG CGAAAGACCA AGTCAAAGAT GCTGCGGCTG ATGCAAAGGC 201 AAGTGCCGAG GAAGCTGTAA CTGAAGCCAA AGAAGCTGTA ACTGAAGCAG 251 CTAAAGATAC TTTGAACAAA GCTGCCGACG CGACTCAGGA AGCGGCAGAC 301 AAAATGAAAG ATGCCGCCAA ATAA This corresponds to the amino acid sequence <SEQ ID 818; ORF 228>: m228.pep 1 MKKLLIAAMM AAALAACSQE AKQEVKEAVQ AVESDVKDTA ASAAESAASA 51 VEEAKDQVKD AAADAKASAE EAVTEAKEAV TEAAKDTLΝK AADATQEAAD 101 KMKDAAK* Computer analysis of this amino acid sequence gave the following results: The following partial DΝA sequence was identified in N meningitidis <SEQ ID 819>: a228.seq 1 ATGAAAAAAT TATTGATTGC CGCAATGATG GCGGCTGCCT TGGCAGCTTG 51 TTCGCAAGAA GCCAAACAGG AGGTTAAGGA AGCGGTTCAA GCCGTTGAGT 101 CCGATGTTAA AGACACTGCG GCTTCTGCCG CCGAGTCTGC CGCTTCTGCC 151 GTCGAAGAAG CGAAAGACCA AGTCAAAGAT GCTGCGGCTG ATGCAAAGGC 201 AAGTGCCGAG GAAGCTGTAA CTGAAGCCAA AGAAGCTGTA ACTGAAGCAG 251 CTAAAGATAC TTTGAACAAA GCTGCCGACG CGACTCAGGA AGCGGCAGAC 301 AAAATGAAAG ATGCCGCCAA ATAA This corresponds to the amino acid sequence <SEQ ID 820; ORF 228. a>: a228 . pep 1 MKKLLIAAMM AAALAACSQE AKQEVKEAVQ AVESDVKDTA ASAAESAASA 51 VEEAKDQVKD AAADAKASAE EAVTEAKEAV TEAAKDTLNK AADATQEAAD 101 KMKDAAK* m228/a228 100.0% identity in 107 aa overlap 10 20 30 40 50 60 m228.pep MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAAESAASAVEEAKDQVKD I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a228 MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAAESAASAVEEAKDQVKD 10 20 30 40 50 60 70 80 90 100 m228. pep AAADAKASAEEAVTEAKEAVTEAAKDTLNKAADATQEAADKMKDAAKX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a228 AAADAKASAEEAVTEAKEAVTEAAKDTLNKAADATQEAADKMKDAAKX 70 80 90 100 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ED 821>: g229.seq 1 atggctgccg tatcgggcgg cggtgcggtc ttcctgataa tgcttccaca 51 tattgcccgc gttcagcgtc agccgccagc gttcgcccaa gcgtcgggag 101 aaatcggcat tgaagccgcc ggcgaaattg tatcggctgc cgcccaagag 151 gttttgcccg acaaacggca cggtgccgaa cgagcgcgtt accgaacggt 201 tttgatggcc gaacgacagg cgcaggttct gttcgctgaa atctttgtta 251 tcccaataat gcacgccgcg gctgatgccg ccgtagagga aatgatgccc 301 gcccgcattg atttcgcgcg acacgcccaa gccgtagcgc aaaccgtgtg 351 ccttttgcgg caggctgtcg gcggttttcg tccagcttct gcccgcaaat 401 tcaatcgttt tttcggacga agcgttgttt atagcggatt aacaaaaatc 451 aggacaaggc ggcgggccgc aggcagtacg gatggtacgg aaccggttcg 501 cccggtgctt ggacgcctta gggaaccgtt ccctttgagc cggggcgggg 551 caacccgtac cggtttttgt tcatccgcca tattgtgttg a This corresponds to the amino acid sequence <SEQ ID 822; ORF 229.ng>: g229.pep 1 MAAVSGGGAV FLIMLPHIAR VQRQPPAFAQ ASGEIGIEAA GEIVSAAAQE 51 VLPDKRHGAE RARYRTVLMA ERQAQVLFAE IFVIPIMHAA ADAAVEEMMP 101 ARIDFARHAQ AVAQTVCLLR QAVGGFRPAS ARKFΝRFFGR SWYSGLTKI 151 RTRRRAAGST DGTEPVRPVL GRLREPFPLS RGGATRTGFC SSAILC* The following partial DΝA sequence was identified in N meningitidis <SEQ ED 823>: m229.seq (partial) 1 ..GCTCAAGCGT TGGGAGAAAT CGGCATTGAA GCCGCCGACG AAATTGTATC 51 GGCTGCCGCC TAAGAGGTTT TGCTCGACAA ACGGCACGAT GCCGAACGAG 101 CGCGTTACCG AACGGTTTTT ATAGCCGAAC GACAGGCGCA GGCTCTGTTC 151 GCTGAAATCT TTGTTATCCC AATAATGCAC GCCGCCGCCG CTGATGCCGC 201 CGTAGAGGAA ATGATGCCTG CCCGCATTGA TTTCGCGCGA CACGCCTAAG 251 CCCTAGCGCA AACCGTGTGC CTTTTGCGGC AGGCTGTCGG CGGTTTTCGT 301 CCAGCTTCTG CCCGCAAATT CAATCGTTTT TTCGGACGAA GCGTTGTTTA 351 TAGCGGATTA ACAAAAATCA GGACAAGGCA ACGAAGCCGC AGACAGTACA 401 AATAGTACGG AACCGATTCA CTTGGTGCTT CAGCACcTTA GAGAATCGTT 451 CTCTTTTTTG TTCATCCGCT ATATTGTGTT GA This corresponds to the amino acid sequence <SEQ ID 824; ORF 229>: m229.pep (partial) 1 . -AQALGEIGIE AADEIVSAAA XEVLLDKRHD AERARYRTVF IAERQAQALF 51 AEIFVIPIMH AAAADAAVEE MMPARIDFAR HAXALAQTVC LLRQAVGGFR 101 PASARKFΝRF FGRS YSGL TKIRTRQRSA DSTΝSTEPIH LVLQHLRESR 151 SLFCSSAILC * Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 229 shows 80.5% identity over a 169 aa overlap with a predicted ORF (ORF 229.ng) from N gonorrhoeae: m229/g209 10 20 30 m229.pep AQALGEIGIEAADEIVSAAAXEVLLDKRHDAE III III MM II g229 MAAVSGGGAVFLIMLPHIARVQRQPPAFAQASGEIGIEAAGEIVSAAAQEVLPDKRHGAE 10 20 30 40 50 60 40 50 60 70 80 90 m229.pep RARYRTVFIAERQAQALFAEIFVIPIMHAAAADAAVEEMMPARIDFARHAXALAQTVCLL I I I I M h M I M I h l l l l l l l l l l l l l l l 1 1 1 I I I I I I I 1 1 1 I I I I I h l l l l l M g229 RARYRTVLMAERQAQVLFAEIFVIPIMHAAA-DAAVEEMMPARIDFARHAQAVAQTVCLL 70 80 90 100 110 100 110 120 130 140 m229. pep RQAVGGFRPASARKFΝRFFGRSWYSGLTKIRTRQRSADSTΝSTEPIHLVLQHLRE MMMMMMMMMMMMMMMMIhhl 11 - 111 = = II MM g229 RQAVGGFRPASARKFΝRFFGRSWYSGLTKIRTRRRAAGSTDGTEPVRPVLGRLREPFPL 120 130 140 150 160 170 150 160 m229.pep SRSLFCSSAILCX = h g229 SRGGATRTGFCSSAILC 180 190 The following partial DΝA sequence was identified in N meningitidis <SEQ TD 825>: a229.seq (partial) 1 ATGGCTGTCG TATCGGGCGG CGGTGCGGTC TTCCTGATAA CGCTTCCACA 51 TATTGCCCAC GTTCAGCGTC AGCCGCCA.. GTTCGCTCAA GCGTCGGGAG 101 AAATCGGCAT TGAAGCCGCC GACGAAATTG TATCGGCTGC CGCCTAAGAG 151 GTTTTGCTCG ATAAACGGCA CGATGCCGAA TGAGCGCGTT ACTGAACGGT 201 TTTTATAGCC GAGCGACAGG CGCAGGCTCT GTTCGCTGAA ATCTTTGTTA 251 TCCTAATAGT GCACGCCGCC GCCGCTGATG TCTCCGTAGA GGAAATGATG 301 CCCGCCCGCA TTGATTTCGC GCGACACGCC CAAGCCGTAG CGCAAACCGT 351 GTGCCTTTTG CGGCAGGCTG TCGGCGGTTT TCGTCCAGCT TCTGCCTGCA 401 AATTCAATCG TTTTTTCGGA CGAAGCGTTG TTTATAGCGG ATTAACAAAA 451 ATCAGGACAA GGCGACGAAG CGCAGACAGT ACAGATAGTA CGGAACCGAT 501 TCACTTGGTG CTTCAGCACC TTAGAGAATC GTCTCTTTGA GCTAAGGCGA 551 GGCAACGCCG TACTGGTTTT TGTTCATCCA CTATA This corresponds to the amino acid sequence <SEQ ID 826; ORF 229.a>: a229.pep (partial) 1 MAWSGGGAV FLITLPHIAH VQRQPPXFAQ ASGEIGIEAA DEIVSAAA*E 51 VLLDKRHDAE *ARY*TVFIA ERQAQALFAE IFVILIVHAA AADVSVEEMM 101 PARIDFARHA QAVAQTVCLL RQAVGGFRPA SACKFΝRFFG RSWYSGLTK 151 IRTRRRSADS TDSTEPIHLV LQHLRESSL* AKARQRRTGF CSSTI m229/a229 85.6% identity in 167 aa overlap 10 20 30 m229 . pep AQALGEIGIEAADEIVSAAAXEVLLDKRHDAE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a229 MAWSGGGAVFLITLPHIAHVQRQPPXFAQASGEIGIEAADEIVSAAAXEVLLDKRHDAE 10 20 30 40 50 60 40 50 60 70 80 90 m229. pep RARYRTVFIAERQAQALFAEIFVIPIMHAAAADAAVEEMMPARIDFARHAXALAQTVCLL III I I I I I I I I I I I I I I I I I I I I : I I I I I I :: I I I I I I I I I I I I I I I I : I I I I I I I a229 XARYXTVFIAERQAQALFAEIFVILIVHAAAADVSVEEMMPARIDFARHAQAVAQTVCLL 70 80 90 100 110 120 100 110 120 130 140 149 m229. pep RQAVGGFRPASARKFNRFFGRSWYSGLTKIRTRQRSADSTNSTEPIHLVLQHLRES I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I : I I I I I I I I I I I I I I I a229 RQAVGGFRPASACKFNRFFGRSWYSGLTKIRTRRRSADSTDSTEPIHLVLQHLRESSLX 130 140 150 160 170 180 150 160 m229.pep RSLFCSSAILCX I : I I I I: I a229 AKARQRRTGFCSSTI 190 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 827>: g230. seq 1 atgttccatt ccatcgaaaa atacagaaca cccgcccaag tcttattagg 51 cctgattgca ttaacttttg tcggcttcgg cgtcagcacg gtttcccatc 101 cgggcgccga ctacatcgtc caagtgggcg acgaaaaaat cagcgagcac 151 tcaatcaaca acgccatgca gaacgagcag gcggacggcg gcagcccttg 201 gcgcgacgcg gtgttccaat ccctgctgca acgcgcctac ctgaaacagg 251 gcgcgaagct gatgggcatt tcggtttctt ccgaacaaat caagcagatg 301 attgtggacg atcccaattt ccacgacgca aacggcaaat tcagtcacgc 351 gcttttgagt caatacctgt cgcaacgcca tatgtctgaa gaccagtttg 401 tcgaagaaat ccgcgatcag tttgccttgc agaatttggt aagcctcgtc 451 caaaacggcg tattggtcgg cgacgcgcag gcggaacagc tgatcaggct 501 gacgcaggtc aaccgcacca tccgttcgca cactttcaac cccgacgagt 551 tcatcgccca agtcaaagcg tctgaagccg atttgcagaa attttataat 601 gcgaacaaaa aagactatct gctgccgcag gcggtcaaat tggaatatgt 651 cgccttgaat ctgaaggatt ttgcagacaa gcagaccgtc agtgaaacgg 701 aagtgaaaaa tgcgtttgaa gagcgcgtgg cgcgtttgcc ggcacatgaa 751 gccaaacctt ctttcgagca ggaaaaagcc gccgtcgaaa acgaattgaa 801 aatgaaaaag gcggttgccg acttcaacaa ggcaaaagaa aagctgggcg 851 acgatgcgtt caatcatccc tcctcgcttg ccgaagccgc caaaaacagc 901 ggtttgaaag tggaaaccca agaaacttgg ctgagcaggc aggacgcaca 951 aatgtccggc atgcccgaaa acctaatcaa tgccgtattc agcgacgacg 1001 tattgaagaa aaaacacaat tccgaagtgc tgaccatcaa cagcgaaacc 1051 gcgtgggtcg tccgcgccaa agaagtccgc gaagaaaaaa acctactgtt 1101 tgaagaagcc aaagatgcgg tgcgtcaggc ctatatccgt accgaagccg 1151 ccaaactttt gaaaacaatg taa This corresponds to the amino acid sequence <SEQ ID 828; ORF 230.ng>: g230.pep 1 MFHSIEKYRT PAQVLLGLIA LTFVGFGVST VSHPGADYIV QVGDEKISEH 51 SIΝΝAMQΝEQ ADGGSPWRDA VFQSLLQRAY LKQGAKLMGI SVSSEQIKQM 101 IVDDPΝFHDA ΝGKFSHALLS QYLSQRHMSE DQFVEEIRDQ FALQΝLVSLV 151 QΝGVLVGDAQ AEQLIRLTQV ΝRTIRSHTFΝ PDEFIAQVKA SEADLQKFYΝ 201 AΝKKDYLLPQ AVKLEYVALΝ LKDFADKQTV SETEVKΝAFE ERVARLPAHE 251 AKPSFEQEKA AVEΝELKMKK AVADFΝKAKE KLGDDAFΝHP SSLAEAAKΝS 301 GLKVETQETW LSRQDAQMSG MPEΝLIΝAVF SDDVLKKKHΝ SEVLTIΝSET 351 AWWRAKEVR EEKΝLLFEEA KDAVRQAYIR TEAAKLLKTM * The following partial DΝA sequence was identified in N meningitidis <SEQ ID 829>: m230.seq (partial) 1 ATGTTCCATT CCATCGAAAA ATACAGAACG CCCGCCCAAG TCCTTTTGGG 51 CCTGATTGCA TTAACCTTCG TCGGCTTCGG GGTCAGCACG GTATCCCATC 101 CGGGTGCCGA CTACATCGTC CAAGTGGGCG ACGAAAAAaT CAGCGACCAC 151 TCCATCAACA ACGCCATACA GAACGAACAG GCGGACGGCG GCGGCCCTTC 201 GCc.GACGCG GTGTTCCAAT CCCTGCTGCA ACGCGCCTAC CTGAAACAGG 251 GCGCGAAGCT GATGGGCATT TCGGTTTCTT CCGAACAAAT CAAGCAAATT 301 ATCGTGGACG ATCCCAATTT CCACGACGCA AACGGCAAAT TCGACCACGC 351 GCTTTTAAAC CGCTACCTTT CCCAACGCCA TATGTCTGAA GACCAGTTTG 401 TCGAAGAAAT CCGCGATCAG TTTGCCTTGC AGAATTTGGT AAACCTCGTC 451 CAAAACGGCG TATTGGTCGG CGACGCGCAG GCGGAACAGC TGATCAGGCT 501 GACACAGGTC AACCGCACCA TCCGTTCGCA CACTTTCAAC CCCGACGAGT 551 TCATCGCCCA AGTCAAAGTG TCTGAAGCCG ATTTGCAGAA ATTTTATAAT 601 GCGAACAAAA AAGACTATCT GCTGCCGCAG gCGGTCAAAT TGGAATATGT 651 CGCCTTGAAT CTGAAGGATT TTGCAGACAA GCAGACCGTC AGTGAAACGg 701 AAGTGAAAAA TGCATTTGAA GAGCGCGTGG CGCGTTTGCC GGCAAATGAA 751 GCCAAACCTT CTTTCGAGCA GGAAAAAGCC GCCGTCGAAA ACGAATTGAA 801 AATGAAAAAG GCGGTTGCCG ACTTCAACAA GGCAAAAGAA AAATTGGGCG 851 ACGATGC.GT cAACCATCCT TCyTCGCTTG CCGAAGCCGC CAAAAACAGC 901 GGTTTGAAAG TCGAAACCCA AGAAACTTGG CTGAGTAGGC AGGACGCGCA 951 AATGTCCGGT ATGCCCGAAA ACCTGATCAA TGCCGTATTC AGCGACGACG 1001 TATTGAAGAA AAAACACAAT TCCGAAGTGC TGACCATCAA CAGCGAAACC 1051 GCGTGGGTCG TCCGCGCCAA AGAAGTCCGC GAAGAGAAAA CCCTGCCGTT 1101 TGCCGAAGCC AAAGACGCGG TACGTCAGGC TTATATCCGT ACCGAAGCCG 1151 CCAAACTT This corresponds to the amino acid sequence <SEQ ID 830; ORF 230>: m230.pep (partial) 1 MFHSIEKYRT PAQVLLGLIA LTFVGFGVST VSHPGADYIV QVGDEKISDH 51 SINNAIQNEQ ADGGGPSPDA VFQSLLQRAY LKQGAKLMGI SVSSEQIKQI 101 IVDDPNFHDA NGKFDHALLN RYLSQRHMSE DQFVEEIRDQ FALQNLVNLV 151 QNGVLVGDAQ AEQLIRLTQV NRTIRSHTFN PDEFIAQVKV SEADLQKFYN 201 ANKKDYLLPQ AVKLEYVALN LKDFADKQTV SETEVKNAFE ERVARLPANE 251 AKPSFEQEKA AVENELKMKK AVADFNKAKE KLGDDAVNHP SSLAEAAKNS 301 GLKVETQETW LSRQDAQMSG MPENLINAVF SDDVLKKKHN SEVLTINSET 351 AWWRAKEVR EEKTLPFAEA KDAVRQAYIR TEAAKL.... Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 230 shows 95.9% identity over a 386 aa overlap with a predicted ORF (ORF 230.ng) from N gonorrhoeae: m230/g230 10 20 30 40 50 60 m230. pep MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGDEKISDHSIΝΝAIQΝEQ I MIIMIIM MM MM II III I II II III I II II III I III II Ihlll II hi III g230 MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGDEKISEHSIΝΝAMQΝEQ 10 20 30 40 50 60 70 80 90 100 110 120 m230.pep ADGGGPSPDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIVDDPΝFHDAΝGKFDHALLΝ lllhl MMMMMMMMMMMMMMMhlMMMIMMIhMlh g230 ADGGSPWRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQMIVDDPΝFHDAΝGKFSHALLS 70 80 90 100 110 120 130 140 150 160 170 180 m230. ep RYLSQRHMSEDQFVEEIRDQFALQΝLVΝLVQΝGVLVGDAQAEQLIRLTQVΝRTIRSHTFΝ g230 QYLSQRHMSEDQFVEEIRDQFALQΝLVSLVQΝGVLVGDAQAEQLIRLTQVΝRTIRSHTFΝ 130 140 150 160 170 180 190 200 210 220 230 240 m230. pep PDEFIAQVKVSEADLQKFYΝAΝKKDYLLPQAVKLEYVALΝLKDFADKQTVSETEVKΝAFE MMMMhlMMMMMMMMMMMMMMMMMMMMIMMMM g230 PDEFIAQVKASEADLQKFYΝAΝKKDYLLPQAVKLEYVALΝLKDFADKQTVSETEVKΝAFE 190 200 210 220 230 240 250 260 270 280 290 300 m230. pep ERVARLPAΝEAKPSFEQEKAAVEΝELKMKKAVADFΝKAKEKLGDDAVΝHPSSLAEAAKΝS MMMMMMMMMMIMMMMMMMMMIMMM MMIMMMM g230 ERVARLPAHEAKPSFEQEKAAVEΝELKMKKAVADFΝKAKEKLGDDAFΝHPSSLAEAAKΝS 250 260 270 280 290 300 310 320 330 340 350 360 m230.pep GLKVETQETWLSRQDAQMSGMPENLINAVFSDDVLKKKHNSEVLTINSETAWWRAKEVR MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g230 GLKVETQETWLSRQDAQMSGMPENLINAVFSDDVLKKKHNSEVLTINSETAWWRAKEVR 310 320 330 340 350 360 370 380 m230. pep EEKTLPFAEAKDAVRQAYIRTEAAKL l l h l I l l l l l l l l l g230 EEKNLLFEEAKDAVRQAYIRTEAAKLLKTM 370 380 390 The following partial DNA sequence was identified inN meningitidis <SEQ ID 831>: a230.seq (partial) 1 ATGTTCCATT CCATCGAAAA ATACAGAACG CCCGCCCAAG TCCTTTTGGG 51 CCTGATTGCA TTAACCTTCG TCGGCTTCGG GGTCAGCACG GTATCCCATC 101 CGGGTGCCGA CTACATCGTC CAAGTGGGCG ACGAAAAAAT CAGCGACCAC 151 TCCATCAACA ACGCCATACA GAACGAACAG GCGGACGGCG GCGGCCCTTC 201 GCGCGACGCG GTGTTCCAAT CCCTGCTACA ACGCGCCTAC CTGAAACAGG 251 GCGCGAAGCT GATGGGCATT TCGGTTTCTT CCGAACAAAT CAAGCAGATT 301 ATCGTGGACG ATCCCAATTT CCACGACGCA AACGGCAAAT TCGACCACGC 351 GCTTTTAAAC CGCTACCTTT CCCAACGTCA TATGTCTGAA GACCAGTTTG 401 TCGAAGAAAT CCGCGATCAG TTTGCCTTGC AGAATTTGGT AAACCTCGTC 451 CAAAACGGCG TATTGGTCGG CGACGCGCAG GCGGAACAGC TGATCAGGCT 501 GACGCAGGTC AACCGCACCA TCCGTTCGCA CACTTTCAAC CCCGACGAAT 551 TCATCGCCCA AGTCAAAGTG TCTGAAGCCG ATTTGCAGAA GTTTTATAAC 601 GCAAACAAAA AAGACTACCT GCTTCCCAAA GCGGTCAAAT TGGAATATGT 651 CGCCTTGAAT CTGAAAGACT TTGCAGACAA ACAGACCGTC AGCGAAACAG 701 AAGTGAAAAA TGCGTTTGAA GAGCGCGTGG CGCGTTTGCC GGCAAATGAA 751 GCCAAACCTT CTTTCGAGCA GGAAAAAGCC GCCGTCGAAA ACGAATTGAA 801 AATGAAAAAG GCGGTTGCCG ACTTCAATAA GGCAAAAGAA AAGCTGGGCG 851 ATGACGCGTT CAACCATCCT TCCTCGCTTG CCGAAGCCGC CAAAAACAGC 901 GGTTTGAAAG TCGAAACCCA AGAAACTTGG CTGAGCAGGC AGGATGCGCA 951 AATGTCCGGT ATGCCCGAAA ACCTGATCAA TGCCGTATTC AGCGACGACG 1001 TATTGAAGAA AAAACACAAT TCCGAAGTGC TGACCATCAA CAGCGAAACC 1051 GCGTGGGTCG TCCGCGCCAA AGAAGTCCGC GAAGAGAAAA CCCTGCCGTT 1101 TGCCGAAGCC AAAGACGCGG TACGTCAGGC TTATATCCGT ACCGAAGCCG 1151 CCAAACTT This corresponds to the amino acid sequence <SEQ ID 832; ORF 230.a>: a230.pep (partial) 1 MFHSIEKYRT PAQVLLGLIA LTFVGFGVST VSHPGADYIV QVGDEKISDH 51 SIΝΝAIQΝEQ ADGGGPSRDA VFQSLLQRAY LKQGAKLMGI SVSSEQIKQI 101 IVDDPΝFHDA ΝGKFDHALLΝ RYLSQRHMSE DQFVEEIRDQ FALQΝLVΝLV 151 QΝGVLVGDAQ AEQLIRLTQV ΝRTIRSHTFΝ PDEFIAQVKV SEADLQKFYΝ 201 AΝKKDYLLPK AVKLEYVALΝ LKDFADKQTV SETEVKΝAFE ERVARLPANE 251 AKPSFEQEKA AVENELKMKK AVADFNKAKE KLGDDAFNHP SSLAEAAKNS 301 GLKVETQETW LSRQDAQMSG MPENLINAVF SDDVLKKKHN SEVLTINSET 351 AWWRAKEVR EEKTLPFAEA KDAVRQAYIR TEAAKL m230/a230 99.2% identity in 386 aa overlap 10 20 30 40 50 60 m230.pep MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGDEKISDHSINNAIQNEQ I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a230 MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGDEKISDHSINNAIQNEQ 10 20 30 40 50 60 70 80 90 100 110 120 m230.pep ADGGGPSPDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIVDDPNFHDANGKFDHALLN lllllll I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I a230 ADGGGPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIVDDPNFHDANGKFDHALLN 70 80 90 100 110 120 130 140 150 160 170 180 m230. pep RYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQAEQLIRLTQVNRTIRSHTFN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I II I I I I I I I I I I I I I I a230 RYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQAEQLIRLTQVNRTIRSHTFN 130 140 150 160 170 180 190 200 210 220 230 240 m230. pep PDEFIAQVKVSEADLQKFYNANKKDYLLPQAVKLEYVALNLKDFADKQTVSETEVKNAFE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a230 PDEFIAQVKVSEADLQKFYNANKKDYLLPKAVKLEYVALNLKDFADKQTVSETEVKNAFE 190 200 210 220 230 240 250 260 270 280 290 300 m230. pep ERVARLPANEAKPSFEQEKAAVENELKMKKAVADFNKAKEKLGDDAVNHPSSLAEAAKNS I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a230 ERVARLPANEAKPSFEQEKAAVENELKMKKAVADFNKAKEKLGDDAFNHPSSLAEAAKNS 250 260 270 280 290 300 310 320 330 340 350 360 m230. pep GLKVETQETWLSRQDAQMSGMPENLINAVFSDDVLKKKHNSEVLTINSETAWWRAKEVR I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a230 GLKVETQETWLSRQDAQMSGMPENLINAVFSDDVLKKKHNSEVLTINSETAWVVRAKEVR 310 320 330 340 350 360 370 380 m230.pep EEKTLPFAEAKDAVRQAYIRTEAAKL I I I I I I I I I I I I I I I I I I I I I I I I I I a230 EEKTLPFAEAKDAVRQAYIRTEAAKL 370 380 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 833>: g230-l.seq 1 ATGTTCCATT CCATCGAAAA ATACAGAACA CCCGCCCAAG TCTTATTAGG 51 CCTGATTGCA TTAACTTTTG TCGGCTTCGG CGTCAGCACG GTTTCCCATC 101 CGGGCGCCGA CTACATCGTC CAAGTGGGCG ACGAAAAAAT CAGCGAGCAC 151 TCAATCAACA ACGCCATGCA GAACGAGCAG GCGGACGGCG GCAGCCCTTG 201 GCGCGACGCG GTGTTCCAAT CCCTGCTGCA ACGCGCCTAC CTGAAACAGG 251 GCGCGAAGCT GATGGGCATT TCGGTTTCTT CCGAACAAAT CAAGCAGATG 301 ATTGTGGACG ATCCCAATTT CCACGACGCA AACGGCAAAT TCAGTCACGC 351 GCTTTTGAGT CAATACCTGT CGCAACGCCA TATGTCTGAA GACCAGTTTG 401 TCGAAGAAAT CCGCGATCAG TTTGCCTTGC AGAATTTGGT AAGCCTCGTC 451 CAAAACGGCG TATTGGTCGG CGACGCGCAG GCGGAACAGC TGATCAGGCT 501 GACGCAGGTC AACCGCACCA TCCGTTCGCA CACTTTCAAC CCCGACGAGT 551 TCATCGCCCA AGTCAAAGCG TCTGAAGCCG ATTTGCAGAA ATTTTATAAT 601 GCGAACAAAA AAGACTATCT GCTGCCGCAG GCGGTCAAAT TGGAATATGT 651 CGCCTTGAAT CTGAAGGATT TTGCAGACAA GCAGACCGTC AGTGAAACGG 701 AAGTGAAAAA TGCGTTTGAA GAGCGCGTGG CGCGTTTGCC GGCACATGAA 751 GCCAAACCTT CTTTCGAGCA GGAAAAAGCC GCCGTCGAAA ACGAATTGAA 801 AATGAAAAAG GCGGTTGCCG ACTTCAACAA GGCAAAAGAA AAGCTGGGCG 851 ACGATGCGTT CAATCATCCC TCCTCGCTTG CCGAAGCCGC CAAAAACAGC 901 GGTTTGAAAG TGGAAACCCA AGAAACTTGG CTGAGCAGGC AGGACGCACA 951 AATGTCCGGC ATGCCCGAAA ACCTAATCAA TGCCGTATTC AGCGACGACG 1001 TATTGAAGAA AAAACACAAT TCCGAAGTGC TGACCATCAA CAGCGAAACC 1051 GCGTGGGTCG TCCGCGCCAA AGAAGTCCGC GAAGAAAAAA ACCTACTGTT 1101 TGAAGAAGCC AAAGATGCGG TGCGTCAGGC CTATATCCGT ACCGAAGCCG 1151 CCAAACTTGC CGAAAACAAG GCAAAAGAAG TGCTTACCCA ACTGAACGGC 1201 GGCAAGGCAG TTGACGTGAA ATGGTCGGAA GTGTCCGTTT TGGGCGCGCA 1251 GCAGGCAAGG CAGTCCATGC CGCCCGAGGC TTATGCGGAA CTGCTGAAAG 1301 CAAAACCGGC AAACGGCAAA CCCGCCTATG TCAGACTGAC CGGTCTGCCG 1351 GCACCCGTGA TTGTCGAGGC GCAGGCAGTC ACGCCTCCGG AGGATATTGC 1401 CGCACAGCTT CCTCCTGCGA AACAGGCTTT GGCGCAACAG CAGTCTGCCA 1451 ATACTTTCGA CCTGCTGATC CGCTATTTCA ACGGAAAAAT CAAACAGACT 1501 AAAGGAGCAC AATCGGTTGA CAACGGCGAT GGTCAGTAA This corresponds to the amino acid sequence <SEQ ID 834; ORF 230-l.ng>: g230-l.pep 1 MFHSIEKYRT PAQVLLGLIA LTFVGFGVST VSHPGADYIV QVGDEKISEH 51 SIΝΝAMQΝEQ ADGGSPWRDA VFQSLLQRAY LKQGAKLMGI SVSSEQIKQM 101 IVDDPΝFHDA ΝGKFSHALLS QYLSQRHMSE DQFVEEIRDQ FALQΝLVSLV 151 QNGVLVGDAQ AEQLIRLTQV NRTIRSHTFN PDEFIAQVKA SEADLQKFYN 201 ANKKDYLLPQ AVKLEYVALN LKDFADKQTV SETEVKNAFE ERVARLPAHE 251 AKPSFEQEKA AVENELKMKK AVADFNKAKE KLGDDAFNHP SSLAEAAKNS 301 GLKVETQETW LSRQDAQMSG MPENLINAVF SDDVLKKKHN SEVLTINSET 351 AWWRAKEVR EEKNLLFEEA KDAVRQAYIR TEAAKLAENK AKEVLTQLNG 401 GKAVDVKWSE VSVLGAQQAR QSMPPEAYAE LLKAKPANGK PAYVRLTGLP 451 APVIVEAQAV TPPEDIAAQL PPAKQALAQQ QSANTFDLLI RYFNGKIKQT 501 KGAQSVDNGD GQ* The following partial DNA sequence was identified in N meningitidis <SEQ ID 835>: m230-l.seq 1 ATGTTCCATT CCATCGAAAA ATACAGAACG CCCGCCCAAG TCCTTTTGGG 51 CCTGATTGCA TTAACCTTCG TCGGCTTCGG GGTCAGCACG GTATCCCATC 101 CGGGTGCCGA CTACATCGTC CAAGTGGGCG ACGAAAAAAT CAGCGACCAC 151 TCCATCAACA ACGCCATACA GAACGAACAG GCGGACGGCG GCGGCCCTTC 201 GCGCGACGCG GTGTTCCAAT CCCTGCTGCA ACGCGCCTAC CTGAAACAGG 251 GCGCGAAGCT GATGGGCATT TCGGTTTCTT CCGAACAAAT CAAGCAAATT 301 ATCGTGGACG ATCCCAATTT CCACGACGCA AACGGCAAAT TCGACCACGC 351 GCTTTTAAAC CGCTACCTTT CCCAACGCCA TATGTCTGAA GACCAGTTTG 401 TCGAAGAAAT CCGCGATCAG TTTGCCTTGC AGAATTTGGT AAACCTCGTC 451 CAAAACGGCG TATTGGTCGG CGACGCGCAG GCGGAACAGC TGATCAGGCT 501 GACACAGGTC AACCGCACCA TCCGTTCGCA CACTTTCAAC CCCGACGAGT 551 TCATCGCCCA AGTCAAAGTG TCTGAAGCCG ATTTGCAGAA ATTTTATAAT 601 GCGAACAAAA AAGACTATCT GCTGCCGCAG GCGGTCAAAT TGGAATATGT 651 CGCCTTGAAT CTGAAGGATT TTGCAGACAA GCAGACCGTC AGTGAAACGG 701 AAGTGAAAAA TGCATTTGAA GAGCGCGTGG CGCGTTTGCC GGCAAATGAA 751 GCCAAACCTT CTTTCGAGCA GGAAAAAGCC GCCGTCGAAA ACGAATTGAA 801 AATGAAAAAG GCGGTTGCCG ACTTCAACAA GGCAAAAGAA AAATTGGGCG 851 ACGATGCGTT CAACCATCCT TCCTCGCTTG CCGAAGCCGC CAAAAACAGC 901 GGTTTGAAAG TCGAAACCCA AGAAACTTGG CTGAGTAGGC AGGACGCGCA 951 AATGTCCGGT ATGCCCGAAA ACCTGATCAA TGCCGTATTC AGCGACGACG 1001 TATTGAAGAA AAAACACAAT TCCGAAGTGC TGACCATCAA CAGCGAAACC 1051 GCGTGGGTCG TCCGCGCCAA AGAAGTCCGC GAAGAGAAAA CCCTGCCGTT 1101 TGCCGAAGCC AAAGACGCGG TACGTCAGGC TTATATCCGT ACCGAAGCCG 1151 CCAAACTTGC CGAAAACAAG GCAAAAGACG TGCTTACCCA ACTGAACGGC 1201 GGCAAGGCTG TTGACGTGAA ATGGTCGGAA GTGTCCGTTT TGGGCGCACA 1251 GCAGGCAAGG CAGTCCATGC CGCCCGAGGC TTATGCGGAA CTGCTGAAAG 1301 CAAAACCGGC AAACGGCAAA CCCGCCTACG TCAGGCTGAT CGGTCTGCCG 1351 GCACCCGTGA TTGTCGAAGT ACAGGCTGTA ACCCCGCCGG ATGATATCGC 1401 CGCACAGCTT CCGCTTGCAA AACAGGCTTT GGCGCAACAG CAGTCTGCCA 1451 ATACTTTCGA CTTGTTGATA CGTTATTTCA ACGGCAAAAT CAAACAGACC 1501 AAAGGAGCGC AATCGGTCGA CAACGGCGAC GGTCAGTAA This corresponds to the amino acid sequence <SEQ ID 836; ORF 230-l>: m230-l.pep 1 MFHSIEKYRT PAQVLLGLIA LTFVGFGVST VSHPGADYIV QVGDEKISDH 51 SIΝΝAIQΝEQ ADGGGPSRDA VFQSLLQRAY LKQGAKLMGI SVSSEQIKQI 101 IVDDPΝFHDA ΝGKFDHALLΝ RYLSQRHMSE DQFVEEIRDQ FALQΝLVΝLV 151 QΝGVLVGDAQ AEQLIRLTQV ΝRTIRΞHTFΝ PDEFIAQVKV SEADLQKFYΝ 201 ANKKDYLLPQ AVKLEYVALN LKDFADKQTV SETEVKNAFE ERVARLPANE 251 AKPSFEQEKA AVENELKMKK AVADFNKAKE KLGDDAFNHP SSLAEAAKNS 301 GLKVETQETW LSRQDAQMSG MPENLINAVF SDDVLKKKHN SEVLTINSET 351 AWWRAKEVR EEKTLPFAEA KDAVRQAYIR TEAAKLAENK AKDVLTQLNG 401 GKAVDVKWSE VSVLGAQQAR QSMPPEAYAE LLKAKPANGK PAYVRLIGLP 451 APVIVEVQAV TPPDDIAAQL PLAKQALAQQ QSANTFDLLI RYFNGKIKQT 501 KGAQSVDNGD GQ* m230-l/g230-l 96.3% identity in 512 aa overlap 10 20 30 40 50 60 m230-1. pep MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGDEKISDHSINNAIQNEQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I : I I I I I I :| I I I g230-l MFHΞIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGDEKISEHSINNAMQNEQ 10 20 30 40 50 60 70 80 90 100 110 120 m230-l.pep ADGGGPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIVDDPNFHDANGKFDHALLN I I I |:l I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I hi I I I I I I I I I I I I |:| I I I: g230-l ADGGΞPWRDAVFQΞLLQRAYLKQGAKLMGISVSSEQIKQMIVDDPNFHDANGKFSHALLS 70 80 90 100 110 120 130 140 150 160 170 180 m230-1. pep RYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQAEQLIRLTQVNRTIRSHTFN : I I I I I I I I I I I I I I I I I I I I I I I I I I: I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g230-l QYLSQRHMSEDQFVEEIRDQFALQNLVSLVQNGVLVGDAQAEQLIRLTQVNRTIRSHTFN 130 140 150 160 170 180 190 200 210 220 230 240 m230-l.pep PDEFIAQVKVΞEADLQKFYNANKKDYLLPQAVKLEYVALNLKDFADKQTVSETEVKNAFE I I I I I I I I hi I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g230-l PDEFIAQVKASEADLQKFYNANKKDYLLPQAVKLEYVALNLKDFADKQTVSETEVKNAFE 190 200 210 220 230 240 250 260 270 280 290 300 m230-l . pep ERVARLPANEAKPSFEQEKAAVENELKMKKAVADFNKAKEKLGDDAFNHPΞSLAEAAKNS I I I I I I I I : I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I II I I g230-l ERVARLPAHEAKPSFEQEKAAVENELKMKKAVADFNKAKEKLGDDAFNHPSSLAEAAKNS 250 260 270 280 290 300 310 320 330 340 350 360 m230-1. pep GLKVETQETWLSRQDAQMSGMPENLINAVFSDDVLKKKHNΞEVLTINSETAWWRAKEVR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I g230-1 GLKVETQETWLSRQDAQMSGMPENLINAVFSDDVLKKKHNSEVLTINSETAWVVRAKEVR 310 320 330 340 350 360 370 380 390 400 410 420 m230-1. pep EEKTLPFAEAKDAVRQAYIRTEAAKLAENKAKDVLTQLNGGKAVDVKWSEVSVLGAQQAR I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I g230-1 EEKNLLFEEAKDAVRQAYIRTEAAKLAENKAKEVLTQLNGGKAVDVKWSEVΞVLGAQQAR 370 380 390 400 410 420 430 440 450 460 470 480 m230-l.pep QSMPPEAYAELLKAKPANGKPAYVRLIGLPAPVIVEVQAVTPPDDIAAQLPLAKQALAQQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I :| I I I I I: I II I I I I g230-l QSMPPEAYAELLKAKPANGKPAYVRLTGLPAPVIVEAQAVTPPEDIAAQLPPAKQALAQQ 430 440 450 460 470 480 490 500 510 m230-l .pep QSANTFDLLIRYFNGKIKQTKGAQSVDNGDGQX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g230-l QSANTFDLLIRYFNGKIKQTKGAQΞVDNGDGQX 490 500 510 The following partial DNA sequence was identified in N. meningitidis <SEQ ED 837>: a230-l. seq 1 ATGTTCCATT CCATCGAAAA ATACAGAACG CCCGCCCAAG TCCTTTTGGG 5 CCTGATTGCA TTAACCTTCG TCGGCTTCGG GGTCAGCACG GTATCCCATC 10 CGGGTGCCGA CTACATCGTC CAAGTGGGCG ACGAAAAAAT CAGCGACCAC 15 TCCATCAACA ACGCCATACA GAACGAACAG GCGGACGGCG GCGGCCCTTC 20 GCGCGACGCG GTGTTCCAAT CCCTGCTACA ACGCGCCTAC CTGAAACAGG 25 GCGCGAAGCT GATGGGCATT TCGGTTTCTT CCGAACAAAT CAAGCAGATT 30 ATCGTGGACG ATCCCAATTT CCACGACGCA AACGGCAAAT TCGACCACGC 35 GCTTTTAAAC CGCTACCTTT CCCAACGTCA TATGTCTGAA GACCAGTTTG 40 TCGAAGAAAT CCGCGATCAG TTTGCCTTGC AGAATTTGGT AAACCTCGTC 45 CAAAACGGCG TATTGGTCGG CGACGCGCAG GCGGAACAGC TGATCAGGCT 50 GACGCAGGTC AACCGCACCA TCCGTTCGCA CACTTTCAAC CCCGACGAAT 55 TCATCGCCCA AGTCAAAGTG TCTGAAGCCG ATTTGCAGAA GTTTTATAAC 60 GCAAACAAAA AAGACTACCT GCTTCCCAAA GCGGTCAAAT TGGAATATGT 65 CGCCTTGAAT CTGAAAGACT TTGCAGACAA ACAGACCGTC AGCGAAACAG 70 AAGTGAAAAA TGCGTTTGAA GAGCGCGTGG CGCGTTTGCC GGCAAATGAA 75 GCCAAACCTT CTTTCGAGCA GGAAAAAGCC GCCGTCGAAA ACGAATTGAA 80 AATGAAAAAG GCGGTTGCCG ACTTCAATAA GGCAAAAGAA AAGCTGGGCG 85 ATGACGCGTT CAACCATCCT TCCTCGCTTG CCGAAGCCGC CAAAAACAGC 90 GGTTTGAAAG TCGAAACCCA AGAAACTTGG CTGAGCAGGC AGGATGCGCA 95 AATGTCCGGT ATGCCCGAAA ACCTGATCAA TGCCGTATTC AGCGACGACG 100 TATTGAAGAA AAAACACAAT TCCGAAGTGC TGACCATCAA CAGCGAAACC 105 GCGTGGGTCG TCCGCGCCAA AGAAGTCCGC GAAGAGAAAA CCCTGCCGTT 110 TGCCGAAGCC AAAGACGCGG TACGTCAGGC TTATATCCGT ACCGAAGCCG 115 CCAAACTTGC CGAAAACAAG GCAAAAGACG TGCTTACCCA ACTGAACGGC 120 GGCAAGGCTG TTGACGTGAA ATGGTCGGAA GTGTCCGTTT TGGGCGCACA 125 GCAGGCAAGG CAGTCCATGC CGCCCGAGGC TTATGCGGAA CTGCTGAAAG 130 CAAAACCGGC AAACGGCAAA CCCGCCTACG TCAGGCTGAT CGGTCTGCCG 135 GCACCCGTGA TTGTCGAAGT ACAGGCTGTA ACCCCGCCGG ATGATATCGC 140 CGCACAGCTT CCGCTTGCAA AACAGGCTTT GGCGCAACAG CAGTCTGCCA 145 ATACTTTCGA CTTGTTGATA CGTTATTTCA ACGGCAAAAT CAAACAGACC 150 AAAGGAGCGC AATCGGTCGA CAACGGCGAC GGTCAGTAA This corresponds to the amino acid sequence <SEQ ID 838; ORF 230-l.a>: a230-l.pep 1 MFHSIEKYRT PAQVLLGLIA LTFVGFGVST VSHPGADYIV QVGDEKISDH 51 ΞINNAIQNEQ ADGGGPSRDA VFQSLLQRAY LKQGAKLMGI SVSSEQIKQI 101 IVDDPNFHDA NGKFDHALLN RYLSQRHMSE DQFVEEIRDQ FALQNLVNLV 151 QNGVLVGDAQ AEQLIRLTQV NRTIRSHTFN PDEFIAQVKV SEADLQKFYN 201 ANKKDYLLPK AVKLEYVALN LKDFADKQTV SETEVKNAFE ERVARLPANE 251 AKPSFEQEKA AVENELKMKK AVADFNKAKE KLGDDAFNHP SSLAEAAKNS 301 GLKVETQETW LSRQDAQMSG MPENLINAVF SDDVLKKKHN SEVLTINSET 351 AWWRAKEVR EEKTLPFAEA KDAVRQAYIR TEAAKLAENK AKDVLTQLNG 401 GKAVDVKWSE VSVLGAQQAR QSMPPEAYAE LLKAKPANGK PAYVRLIGLP 451 APVIVEVQAV TPPDDIAAQL PLAKQALAQQ QSANTFDLLI RYFNGKIKQT 501 KGAQSVDNGD GQ* a230-l/πι230-l 99.8% identity in 512 aa overlap 10 20 30 40 50 60 a230-l.pep MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGDEKISDHSINNAIQNEQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m230-l MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGDEKISDHSINNAIQNEQ 10 20 30 40 50 60 70 80 90 100 110 120 a230-l . pep ADGGGPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIVDDPNFHDANGKFDHALLN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I m230-1 ADGGGPSRDAVFQΞLLQRAYLKQGAKLMGISVSSEQIKQIIVDDPNFHDANGKFDHALLN 70 80 90 100 110 120 130 140 150 160 170 180 a230-l . pep RYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQAEQLIRLTQVNRTIRSHTFN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I m230-l RYLΞQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQAEQLIRLTQVNRTIRSHTFN 130 140 150 160 170 180 190 200 210 220 230 240 a230-1. pep PDEFIAQVKVSEADLQKFYNANKKDYLLPKAVKLEYVALNLKDFADKQTVΞETEVKNAFE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m230-1 PDEFIAQVKVSEADLQKFYNANKKDYLLPQAVKLEYVALNLKDFADKQTVΞETEVKNAFE 190 200 210 220 230 240 250 260 270 280 290 300 a230-l . pep ERVARLPANEAKPSFEQEKAAVENELKMKKAVADFNKAKEKLGDDAFNHPSSLAEAAKNS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m230-l ERVARLPANEAKPSFEQEKAAVENELKMKKAVADFNKAKEKLGDDAFNHPSSLAEAAKNS 250 260 270 280 290 300 310 320 330 340 350 360 a230-l.pep GLKVETQETWLSRQDAQMΞGMPENLINAVFSDDVLKKKHNSEVLTINΞETAWWRAKEVR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m230-l GLKVETQETWLSRQDAQMSGMPENLINAVFSDDVLKKKHNSEVLTINSETAWWRAKEVR 310 320 330 340 350 360 370 380 390 400 410 420 a230-l.pep EEKTLPFAEAKDAVRQAYIRTEAAKLAENKAKDVLTQLNGGKAVDVKWSEVSVLGAQQAR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I m230-l EEKTLPFAEAKDAVRQAYIRTEAAKLAENKAKDVLTQLNGGKAVDVKWΞEVΞVLGAQQAR 370 380 390 400 410 420 430 440 450 460 470 480 a230-l.pep QSMPPEAYAELLKAKPANGKPAYVRLIGLPAPVIVEVQAVTPPDDIAAQLPLAKQALAQQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I m230-1 QSMPPEAYAELLKAKPANGKPAYVRLIGLPAPVIVEVQAVTPPDDIAAQLPLAKQALAQQ 430 440 450 460 470 480 490 500 510 a230-l .pep QSANTFDLLIRYFNGKIKQTKGAQSVDNGDGQX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m230-l QSANTFDLLIRYFNGKIKQTKGAQSVDNGDGQX 490 500 510 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 839>: g231.seq 1 atgtcaaaac gaaaatccat aaaccgtccg tatcaaaaac cggcggaact 51 gccgccgttg caaaataatc cgccatttta ccgtaaaaac cgccgcctga 101 acttttttat cgcggcagac ggcggttgcg cgtctccgca aaaatgcagg 151 gcgcgcggtt ttcagacggc atttgccgtt caaggccgtg cggtgtcttt 201 accaaatgcc caaccattcg cccacggaat ccatccaatc cttattgccc 251 ccgccgctcc tgcctgcccg gcggtacgcc cacggcgctt gcggattttt 301 agctttccac aatcctttgc gttccctttc cgcctgaatt tgagcgtcgg 351 catagtcggc aaaatccgcc ttatcctgct gttctttagc ataactttta 401 taatgccacg ccgccccgtc ctgcacctgc atcaggttca aatcggtttt 451 gccggcggat acctgcgcca cttcgcgctg atagcggtcg gtttcaaaca 501 cacgtacact gactttccta ccctccgccg ccgcgcgcag gttgtcgcgc 551 gaacgtgtac cgtaagcctg tttcatctcc ggtgcgtcga tatacgccat 601 ccgaatttta tgtttcgcgc cgtcgccgtc gatgacgtga agggtatcgc 651 cgtcatagac tttggacacc gtgcctgtgt agctgtggcc ggatttcgcc 701 gatgcccgtc ggcgaacggg cgcgtcgaaa cccacgtccc ctgcagtgcc 751 gagtacgtcg agtacggcaa ccgccgtccg caccgcctca ctgtcatatc 801 ccgtataacc caacgcgccc aaaagcgaca gggcgacggg aagccatttc 851 atgatttttt taatctgcat atttttcaaa tgccgatgcc gtctgaacat 901 ctctga This corresponds to the amino acid sequence <SEQ ID 840; ORF 231.ng>: g231.pep 1 MSKRKSIΝRP YQKPAELPPL QΝΝPPFYRKΝ RRLΝFFIAAD GGCASPQKCR 51 ARGFQTAFAV QGRAVSLPΝA QPFAHGIHPI LIAPAAPACP AVRPRRLRIF 101 SFPQSFAFPF RLΝLSVGIVG KIRLILLFFS ITFIMPRRPV LHLHQVQIGF 151 AGGYLRHFAL IAVGFKHTYT DFPTLRRRAQ WARTCTVSL FHLRCVDIRH 201 PΝFMFRAVAV DDVKGIAVID FGHRACVAVA GFRRCPSAΝG RVETHVPCSA 251 EYVEYGΝRRP HRLTVISRIT QRAQKRQGDG KPFHDFFΝLH IFQMPMPSEH 301 L* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 841>: m231.seq (partial) 1 ATGTCAAAAC GAAAATCCAT AAACCGTCCG TATCAAAAAC CGGCGGAACT 51 GCCGCCGTTG CAAAATAATC CGCCATTTTA CCGTAAAAAC CGCCGCCTGA 101 ACTTTTTTAT CGCGGCAGAC GGCGGTTGCG CGTCTCCGCA AAAATGCAGG 151 GCGCGCGGTT TTCAGACGGC ATTTGCCGTT CAAAGCCGTG CGGTGTCTTT 201 ACCAAATGCC CAACCATTCG GC This corresponds to the amino acid sequence <SEQ ID 842; ORF 231>: m231.pep (partial) 1 MSKRKSIΝRP YQKPAELPPL QΝΝPPFYRKΝ RRLΝFFIAAD GGCASPQKCR 51 ARGFQTAFAV QSRAVSLPΝA QPFG Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 231 shows 98.6% identity over a 73 aa overlap with a predicted ORF (ORF 23 l.ng) from N gonorrhoeae: m231/g231 10 20 30 40 50 60 m231. pep MSKRKSIΝRPYQKPAELPPLQΝΝPPFYRKΝRRLΝFFIAADGGCASPQKCRARGFQTAFAV M M M M M M M M I M M M M M M M M M M M M M M M M M I M M M M g231 MSKRKSIΝRPYQKPAELPPLQΝΝPPFYRKΝRRLΝFFIAADGGCASPQKCRARGFQTAFAV 10 20 30 40 50 60 70 m231 . pep QSRAVSLPΝAQPFG h i 1 1 1 1 1 1 1 1 I g231 Q GRAVSLPΝAQPFAHGIHPILIAPAAPACPAVRPRRLRIFSFPQSFAFPFRLΝLSVGIVG 70 80 90 100 110 120 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 843>: a231.seq (partial) 1 ATGTCAAAAC GAAAATCCAT AAACCGTCCG TATCAAAAAC CGGCGGAACT 51 GCCGCCGTTG CAAAATAATC CGCCATTTTA CCGTAAAAAC CGCCGCCTGA 101 ACTTTTTTAT CGNGGCAGAC GGCGGTTGCG CGTCTCCGCA AAAATGCAGG 151 GCGCGCGGTT TTCAGACGGC ATTTGCCGTT CAAAGCCGTG CGGTGTCTTT 201 ACCAAATGCC CAACCATTCG CCCACGGCAT CCATCCAATC CTTATTGCCC 251 CCGCCGCTCC TGCCTGCCCG GCGGTACGCC CACGGCGCTT GCGGATTTTT 301 AGCTTTCCAC AATCCTTTGC GTTCCCTTTC CGCCTGAATT TGAGCGTCGG 351 CATAATCGGC AAAATCCGCC TTATCCTGCT GTTCTTTAGC ATAACTTTTA 401 TAATGCCACG CCGCCCCGTC CTGCACCTGC ATCAGGTTCA AATCGGTTTT 451 GCCGACAGAA ACCTGCGCCA CTTCGCGCTG GTAGCGGTCG GTGTCGAACA 501 CGCGGACGCT GACTTTCCTG CCTTCCGCCG CCGCGCGCAG GTTGTCGCGC 551 GAACGCGTGC CGTAAGCCTG TTTCATCTCC GGCGCGTCGA TATACGCCAT 601 CCGGATTTTG TGTTTCGCGC CGTCGCCGTC GATAACGTGA AGGGTGTCGC 651 CGTCATAGAC TTTGGACACC GTGCCTGTGT AGCGGTGGCC GGATTTCGCC 701 GATGCTCGGC GGCGGGCGGG CGCGTCGGAA CCCGCGTCCC CTGCCGCGCC 751 GAGTACGTCG AGTACGGCAA CCGCCGTCCG CACCGCCTCG CTGCCGTACC 801 CCGTATAACC CAACGCACCC AAAAGCGACA AGGCGACGGG AAGCCATTTC 851 ATGATTTTTT TAATCTGCAT ATTTTTCAAA TGCCGATGCC GTCTGAACAT 901 ATC corresponds to the amino acid sequence <SEQ ID 844; ORF 217.a>: a231.pep (partial) 1 MSKRKSINRP YQKPAELPPL QNNPPFYRKN RRLNFFIXAD GGCASPQKCR 51 ARGFQTAFAV QSRAVSLPNA QPFAHGIHPI LIAPAAPACP AVRPRRLRIF 101 SFPQSFAFPF RLNLSVGIIG KIRLILLFFS ITFIMPRRPV LHLHQVQIGF 151 ADRNLRHFAL VAVGVEHADA DFPAFRRRAQ WARTRAVSL FHLRRVDIRH 201 PDFVFRAVAV DNVKGVAVID FGHRACVAVA GFRRCSAAGG RVGTRVPCRA 251 EYVEYGNRRP HRLAAVPRIT QRTQKRQGDG KPFHDFFNLH IFQMPMPSEH 301 I m231/a231 98.6% i i iddeennttiittyy i inn 7733 aaaa oovveerrllaapp 1 1 00 2200 30 40 50 60 m231 . pep MSKRKSINRPYQKPAELPPLQNNPPFYRKNRRLNFFIAADGGCASPQKCRARGFQTAFAV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a231 MSKRKSINRPYQKPAELPPLQNNPPFYRKNRRLNFFIXADGGCASPQKCRARGFQTAFAV 10 20 30 40 50 60 70 m231.pep QSRAVSLPNAQPFG I I I I I I I I I I I I I : a231 QSRAVSLPNAQPFAHGIHPILIAPAAPACPAVRPRRLRIFSFPQSFAFPFRLNLSVGIIG 70 80 90 100 110 120 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 845>: g231-l.seq 1 ATGTCAAAAC GAAAATCCAT AAACCGTCCG TATCAAAAAC CGGCGGAACT 51 GCCGCCGTTG CAAAATAATC CGCCATTTTA CCGTAAAAAC CGCCGCCTGA 101 ACTTTTTTAT CGCGGCAGAC GGCGGTTGCG CGTCTCCGCA AAAATGCAGG 151 GCGCGCGGTT TTCAGACGGC ATTTGCCGTT CAAGGCCGTG CGGTGTCTTT 201 ACCAAATGCC CAACCATTCG CCCACGGAAT CCATCCAATC CTTATTGCCC 251 CCGCCGCTCC TGCCTGCCCG GCGGTACGCC CACGGCGCTT GCGGATTTTT 301 AGCTTTCCAC AATCCTTTGC GTTCCCTTTC CGCCTGAATT TGAGCGTCGG 351 CATAGTCGGC AAAATCCGCC TTATCCTGCT GTTCTTTAGC ATAACTTTTA 401 TAATGCCACG CCGCCCCGTC CTGCACCTGC ATCAGGTTCA AATCGGTTTT 451 GCCGGCGGAT ACCTGCGCCA CTTCGCGCTG ATAGCGGTCG GTTTCAAACa 501 CaCgTaCaat gagtttcgtA ccctccGCCG ccgcgcgCAG GTTGtcgcGC 551 GAACgTGTAC CGTAagcgtg TTtcatctcc GGTGCgtcGA TATACGCCaT 601 cCgAATTTta tGTttcgcgc cgtcgcCgtc gATGACGTGA AGGGtatcGC 651 CgtcATAGAC TTTGGACACC Gtgcctgcgt AGctGTGGCC GGATttcgc This corresponds to the amino acid sequence <SEQ ID 846; ORF 231-l.ng>: g231-l.pep 1 MSKRKSIΝRP YQKPAELPPL QΝΝPPFYRKΝ RRLΝFFIAAD GGCASPQKCR 51 ARGFQTAFAV QGRAVΞLPΝA QPFAHGIHPI LIAPAAPACP AVRPRRLRIF 101 SFPQSFAFPF RLNLSVGIVG KIRLILLFFS ITFIMPRRPV LHLHQVQIGF 151 AGGYLRHFAL IAVGFKHTYN EFRTLRRRAQ WARTCTVSV FHLRCVDIRH 201 PNFMFRAVAV DDVKGIAVID FGHRACVAVA GFR The following partial DNA sequence was identified in N meningitidis <SEQ ID 847>: -l.seq 1 ATGTCAAAAC GAAAATCCAT AAACCGTCCG TATCAAAAAC CGGCGGAACT 51 GCCGCCGTTG CAAAATAATC CGCCATTTTA CCGTAAAAAC CGCCGCCTGA 101 ACTTTTTTAT CGCGGCAGAC GGCGGTTGCG CGTCTCCGCA AAAATGCAGG 151 GCGCGCGGTT TTCAGACGGC ATTTGCCGTT CAAAGCCGTG CGGTGTCTTT 201 ACCAAATGCC CAACCATTCG CCCACGGCAT CCATCCAATC CTTATTGCCC 251 CCGCCGCTCC TGCCTGCTCG GCGGTACGCC CACGGCGCTT GCGGATTTTT 301 AGCTTTCCAC AATCCTTTGC GTTCCCTTTC CGCCTGAATT TGAGCGTCGG 351 CATAATCGGC AAAATCCGCC TTATCCTGCT GTTCTTTAGC ATAACTTTTA 401 TAATGCCACG CCGCCCCGTC CTGCACCTGC ATCAGGTTCA AATCGGTTTT 451 GCCGACAGAA ACCTGCGCCA CTTCGCGCTG GTAGCGGTCG GTATCGAACA 501 CGCGCACGCT GACTTTCCTG CCTTCCGCCG CCGCGCGCAG GTTGTCGCGC 551 GAACGCGTGC CGTAAGCCTG TTTCATCTCC GGCGCGTCGA TATACGCCAT 601 CCGGATTTTG TGTTTCGCGC CGTCGCCGTC GATAACGTGA AGGGTGTCGC 651 CGTCATAGAC TTTGGACACC GTGCCTGTGT AGCGGTGGCC GGATTTCGCC 701 GATGCTCGGC GGCGGGCGGG CGCGTCGGAA CCCGCGTCCC CTGCCGCGCC 751 GAGTACGTCG AGTACGGCAA CCGCCGTCCG CACCGCCTCG CTGCCGTACC 801 CCGTATAACC CAACGCACCC AAAAGCGACA GGGCGACGGG AAGCCATTTC 851 ATGATTTTTT TAATCTGCAT ATTTTTCAAA TGCCGATGCC GTCTGAACAT 901 ATCGGAATCG GATTTCAGAC GGCATCTTAA This corresponds to the amino acid sequence <SEQ ID 848; ORF 231-1>: m231-l.pep 1 MSKRKSIΝRP YQKPAELPPL QΝΝPPFYRKΝ RRLΝFFIAAD GGCASPQKCR 51 ARGFQTAFAV QSRAVSLPΝA QPFAHGIHPI LIAPAAPACS AVRPRRLRIF 101 SFPQSFAFPF RLΝLSVGIIG KIRLILLFFS ITFIMPRRPV LHLHQVQIGF 151 ADRΝLRHFAL VAVGIEHAHA DFPAFRRRAQ WARTRAVΞL FHLRRVDIRH 201 PDFVFRAVAV DΝVKGVAVID FGHRACVAVA GFRRCΞAAGG RVGTRVPCRA 251 EYVEYGΝRRP HRLAAVPRIT QRTQKRQGDG KPFHDFFΝLH IFQMPMPSEH 301 IGIGFQTAS* g231-l/m231-l 87.0% identity in 262 aa overlap 10 20 30 40 50 60 g231-l.pep MSKRKSIΝRPYQKPAELPPLQΝΝPPFYRKΝRRLΝFFIAADGGCASPQKCRARGFQTAFAV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m231-l MSKRKSIΝRPYQKPAELPPLQΝΝPPFYRKΝRRLΝFFIAADGGCASPQKCRARGFQTAFAV 10 20 30 40 50 60 70 80 90 100 110 120 g231-l.pep QGRAVΞLPΝAQPFAHGIHPILIAPAAPACPAVRPRRLRIFSFPQΞFAFPFRLΝLΞVGIVG I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I m231-l QSRAVSLPΝAQPFAHGIHPILIAPAAPACΞAVRPRRLRIFSFPQSFAFPFRLΝLSVGIIG 70 80 90 100 110 120 130 140 150 160 170 180 g231-l.pep KIRLILLFFSITFIMPRRPVLHLHQVQIGFAGGYLRHFALIAVGFKHTYΝEFRTLRRRAQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I :| I I ::| :: :| :: I I I I I m231-l KIRLILLFFSITFIMPRRPVLHLHQVQIGFADRΝLRHFALVAVGIEHAHADFPAFRRRAQ 130 140 150 160 170 180 190 200 210 220 230 240 g231-l . pep WARTCTVSVFHLRCVDIRHPΝFMFRAVAVDDVKGIAVIDFGHRACVAVAGFRXCPSAΝG I I I I I : | I : | I I I I I I I I I : I : I I I I I I I : I I I : I I I I I I I I I I I I I I I I I I : I : I m231-l WARTRAVSLFHLRRVDIRHPDFVFRAVAVDΝVKGVAVIDFGHRACVAVAGFRRCΞAAGG 190 200 210 220 230 240 250 260 g231 - 1 . pep CVETHVPCSAE YWXGΝRRPHR I I : I I I I I I I l l l l l l l m231 - 1 RVGTRVPCRAEYVEYGΝRRPHRLAAVPRITQRTQKRQGDGKPFHDFFΝLHI FQMPMPSEH 250 260 270 280 290 300 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 849>: a231-l.seq 1 ATGTCAAAAC GAAAATCCAT AAACCGTCCG TATCAAAAAC CGGCGGAACT 51 GCCGCCGTTG CAAAATAATC CGCCATTTTA CCGTAAAAAC CGCCGCCTGA 101 ACTTTTTTAT CGCGGCAGAC GGCGGTTGCG CGTCTCCGCA AAAATGCAGG 151 GCGCGCGGTT TTCAGACGGC ATTTGCCGTT CAAAGCCGTG CGGTGTCTTT 201 ACCAAATGCC CAACCATTCG CCCACGGCAT CCATCCAATC CTTATTGCCC 251 CCGCCGCTCC TGCCTGCCCG GCGGTACGCC CACGGCGCTT GCGGATTTTT 301 AGCTTTCCAC AATCCTTTGC GTTCCCTTTC CGCCTGAATT TGAGCGTCGG 351 CATAATCGGC AAAATCCGCC TTATCCTGCT GTTCTTTAGC ATAACTTTTA 401 TAATGCCACG CCGCCCCGTC CTGCACCTGC ATCAGGTTCA AATCGGTTTT 451 GCCGACAGAA ACCTGCGCCA CTTCGCGCTG GTAGCGGTCG GTGTCGAACA 501 CGCGGACGCT GACTTTCCTG CCTTCCGCCG CCGCGCGCAG GTTGTCGCGC 551 GAACGCGTGC CGTAAGCCTG TTTCATCTCC GGCGCGTCGA TATACGCCAT 601 CCGGATTTTG TGTTTCGCGC CGTCGCCGTC GATAACGTGA AGGGTGTCGC 651 CGTCATAGAC TTTGGACACC GTGCCTGTGT AGCGGTGGCC GGATTTCGCC 701 GATGCTCGGC GGCGGGCGGG CGCGTCGGAA CCCGCGTCCC CTGCCGCGCC 751 GAGTACGTCG AGTACGGCAA CCGCCGTCCG CACCGCCTCG CTGCCGTACC 801 CCGTATAACC CAACGCACCC AAAAGCGACA AGGCGACGGG AAGCCATTTC 851 ATGATTTTTT TAATCTGCAT ATTTTTCAAA TGCCGATGCC GTCTGAACAT 901 ATCGGAATCG GATTTCAGAC GGCATCTTAA This corresponds to the amino acid sequence <SEQ ID 850; ORF 231-l.a>: a231-l.pep 1 MSKRKSINRP YQKPAELPPL QNNPPFYRKN RRLNFFIAAD GGCASPQKCR 51 ARGFQTAFAV QSRAVSLPNA QPFAHGIHPI LIAPAAPACP AVRPRRLRIF 101 SFPQSFAFPF RLNLSVGIIG KIRLILLFFS ITFIMPRRPV LHLHQVQIGF 151 ADRNLRHFAL VAVGVEHADA DFPAFRRRAQ WARTRAVSL FHLRRVDIRH 201 PDFVFRAVAV DNVKGVAVID FGHRACVAVA GFRRCSAAGG RVGTRVPCRA 251 EYVEYGNRRP HRLAAVPRIT QRTQKRQGDG KPFHDFFNLH IFQMPMPSEH 301 IGIGFQTAΞ* a231-l/m231-l 99.0% identity in 309 aa overlap 10 20 30 40 50 60 a231-l.pep MSKRKSINRPYQKPAELPPLQNNPPFYRKNRRLNFFIAADGGCAΞPQKCRARGFQTAFAV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m231-l MSKRKSINRPYQKPAELPPLQNNPPFYRKNRRLNFFIAADGGCASPQKCRARGFQTAFAV 10 20 30 40 50 60 70 80 90 100 110 120 a231-l.pep QΞRAVSLPNAQPFAHGIHPILIAPAAPACPAVRPRRLRIFSFPQSFAFPFRLNLSVGIIG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m231-l QSRAVΞLPNAQPFAHGIHPILIAPAAPACSAVRPRRLRIFΞFPQSFAFPFRLNLSVGIIG 70 80 90 100 110 120 130 140 150 160 170 180 a231-1. pep KIRLILLFFSITFIMPRRPVLHLHQVQIGFADRNLRHFALVAVGVEHADADFPAFRRRAQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I m231-l KIRLILLFFSITFIMPRRPVLHLHQVQIGFADRNLRHFALVAVGIEHAHADFPAFRRRAQ 130 140 150 160 170 180 190 200 210 220 230 240 a231-l . pep WARTRAVSLFHLRRVDIRHPDFVFRAVAVDNVKGVAVIDFGHRACVAVAGFRRCΞAAGG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m231-l WARTRAVΞLFHLRRVDIRHPDFVFRAVAVDNVKGVAVIDFGHRACVAVAGFRRCΞAAGG 190 200 210 220 230 240 250 260 270 280 290 300 a231-1 . pep RVGTRVPCRAEYVEYGNRRPHRLAAVPRITQRTQKRQGDGKPFHDFFNLHI FQMPMPSEH I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m231-l RVGTRVPCRAEYVEYGNRRPHRLAAVPRITQRTQKRQGDGKPFHDFFNLHIFQMPMPSEH 250 260 270 280 290 300 310 a231-l.pep IGIGFQTASX I I I I I I I I I I m231-l IGIGFQTASX 310 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 851>: g232.seq 1 atgatgggca acagcctgat tgaatccggt acgtttgtcg ccatcctgtt 51 tggtcagatt ttgggaacgg cggttgccgg cgcgccgcct tatattgtcg 101 ggatactggt tttgctggtc gccgtcggag gaacggccgg cagcctgttt 151 atgccgtccg tacccgccaa ggctgccgat acccaaatcg agtggaatat 201 tgtccgtggt acaaaatccc tgctgcgtga aacggtgcgg cacaatcccg 251 tttttaccgc cattatcggc atctcgtggt tttggtttgt cggcgcggtt 301 tataccacgc aactgccgac ctttacccaa atccatttgg gcggcaacga 351 taatgttttt aacctgatgc ttgctttgtt ttccatcggt attgccgccg 401 gttcggtact gtgtgccaag ttcggcaggg aacggctgat gttggcttgg 451 gtaacggttg gtgcgttggg ttcgacggtt tgcggcctgg ttttggtgtg 501 gctgacgcac ggacaccgtt ttgaagggct gaacggcatt ttttggtttt 551 tatcgcaagg atgggcatac cccgtgatgg cggtgatgac gctgatcggc 601 tttttcggcg gatttttctc cgttccgctc tatacctggc tgcaaaccgc 651 cagcagcgag actttccgcg cccgcgccgt tgccgccaac aatatcgtta 701 acggcatctt tatggtttcc gccgccgttt tgagcgcggt attgctgttt 751 ttgtttgaca gcatttccct gctgtatctg attgtcgcct tgggcaatat 801 tccgttggcg gtatttttga ttaagcgcga aaggcggttt ttaggcgcgg 851 cggcaatcag gaaaaaacct tga This corresponds to the amino acid sequence <SEQ ID 852; ORF 232.ng>: g232.pep 1 MMGNSLIESG TFVAILFGQI LGTAVAGAPP YIVGILVLLV AVGGTAGSLF 51 MPSVPAKAAD TQIEWNIVRG TKSLLRETVR HNPVFTAIIG ISWFWFVGAV 101 YTTQLPTFTQ IHLGGNDNVF NLMLALFSIG IAAGSVLCAK FGRERLMLAW 151 VTVGALGSTV CGLVLVWLTH GHRFEGLNGI FWFLSQGWAY PVMAVMTLIG 201 FFGGFFSVPL YTWLQTASSE TFRARAVAAN NIVNGIFMVS AAVLSAVLLF 251 LFDSISLLYL IVALGNIPLA VFLIKRERRF LGAAAIRKKP * The following partial DNA sequence was identified in N meningitidis <SEQ ID 853>: m232. seq 1 ATGATGGGCA ACAGCCTGAT TGAATCGGGT ACGTTTGTCG CCATCCTGTT 51 CGGTCAGATT TTGGGAACGG CGGTGGCAGG TGTACCGCCT TATATTGTCG 101 GGATACTGGT TTTGCTGGTC GCCGTCGGAG GCACGGTCGG CAGCCTGTTT 151 ATGCCGTCCG TACCCGCCAA GGCTGCCGAT ACACAAATTG AGTGGAATAT 201 TGTCCGTGGC ACAAAATCCC TGCTGCGTGA AACGGTGCGG CACAAGCCCG 251 TTTTTACCGC CATTATCGGT ATTTCGTGGT TTTGGTTTGT CGGCGCGGTT 301 TATACCACGC AACTGCCGAC CTTTACCCAA ATCCATCTGG GCGGCAACGA 351 CAATGTTTTC AACCTGATGC TTGCTCTGTT TTCCATCGGT ATTGCCGCCG 401 GTTCGGTACT GTGTGCCAAG TTCAGCA GG AACGCCTGAT GTTGGCTTGG 451 GTAACGGTTG GTGCGTTGGG TTTGACGGTT TGCGGCTTGG TTTTGGTGTG 501 GCTGACGCAC GGACACCGTT TTGAAGGGCT GAACGGCATT TTTTrGTTTT 551 TATCGCAAGG ATGGGCATAT CCCGTGATGG CGGTGATGAC GCTGATCGGC 601 TTTTTCGGCG GATTTTTCTC CGTTCCGCTC TATACCt(g)TG CAAACCGCCa 651 TAGCGAGaTT TCCGCGCCCg GCCGTTGCCG CCAACAATAT CGTTAACGGT 701 ATTTTTATGG TTTCCGCTGC CGTTTTGAGC GCGGTGTTGC TGTTTTTGTT 751 TGACAGCATT TCCTTGTTGT ATCTGATTGT CGCTTTGGGC AATATTCCGT 801 TGTCGGTATT TTTGATTAAG CGCGAAAGGC GGTTTTTAGG CGCGGCGGCA 851 ATCAGGAAAA AACCTTGA This corresponds to the amino acid sequence <SEQ ID 854; ORF 232>: m232.pep 1 MMGNSLIESG TFVAILFGQI LGTAVAGVPP YIVGILVLLV AVGGTVGSLF 51 MPSVPAKAAD TQIEWNIVRG TKSLLRETVR HKPVFTAIIG ISWFWFVGAV 101 YTTQLPTFTQ IHLGGNDNVF NLMLALFSIG IAAGSVLCAK FSXERLMLAW 151 VTVGALGLTV CGLVLVWLTH GHRFEGLNGI FXFLSQGWAY PVMAVMTLIG 201 FFGGFFSVPL YTVQTAIARF PRPAVAANNI VNGIFMVSAA VLSAVLLFLF 251 DSISLLYLIV ALGNIPLSVF LIKRERRFLG AAAIRKKP* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 232 shows 94.1% identity over a 290 aa overlap with a predicted ORF (ORF 232.ng) from N gonorrhoeae: m232/g232 10 20 30 40 50 60 m232.pep MMGΝSLIESGTFVAILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAAD I M I I I I I I I I M I I I I I I I I I I I I I h l l l l l l l l l l l l l M l h l l l l l l l l l l l l l l g232 MMGΝSLIESGTFVAILFGQILGTAVAGAPPYIVGILVLLVAVGGTAGSLFMPSVPAKAAD 10 20 30 40 50 60 70 80 90 100 110 120 m232.pep TQIEWNIVRGTKSLLRETVRHKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVF I I I I I I I I I I M M I I I I I I h l l I I M i l l I I 1 1 M M l l l l l l l l l l l l l l l I I I I I I g232 TQIEWNIVRGTKSLLRETVRHNPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVF 70 80 90 100 110 120 130 140 150 160 170 180 m232.pep NLMLALFSIGIAAGSVLCAKFSXERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGI M M M M M M I M M M I h M M M M M M M I I I I I I I I I I I I I I I I I I I I I I g232 NLMLALFSIGIAAGSVLCAKFGRERLMLAWVTVGALGSTVCGLVLVWLTHGHRFEGLNGI 130 140 150 160 170 180 190 200 210 220 230 m232.pep FXFLSQGWAYPVMAVMTLIGFFGGFFSVPLYT-VQTAIARFPRP-AVAANNIVNGIFMVS I MMMMMMMMMMMMMMM MM - I MMMMIMMM g232 FWFLSQGWAYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVS 190 200 210 220 230 240 240 250 260 270 280 289 m232.pep AAVLSAVLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKPX MMMMMMMMMMMMMMhlMMMMIMMMMM g232 AAVLSAVLLFLFDSISLLYLIVALGNIPLAVFLIKRERRFLGAAAIRKKP 250 260 270 280 290 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 855>: a232.seq 1 ATGTACGCTA AAAAAGGCGG TTTGGGACTG GTTAAAAGCC GCCGTTTCGC 51 ACCTCTTTTC GCTACGCAGT TTCTCGGCGC GTTCAACGAC AATGTGTTCA 101 AAACCGCGCT GTTTGTGATG ATTGGGTTTT ACGGTTTGGG GCAAAACGGC 151 TTCCTGCCTG CCGGACAGAT GTTGAACTTG GGCGCGTTGC TGTTTATTTT 201 GCCGTATTTC CTGTTTTCCT CGCTGTCGGG GCAGTTGGGT AACAAATTCG 251 ACAAGGCCGT TTTGGCGCGT TGGGCCAAGG TGCTGGAAAT GATCATTATG 301 GCGGTGGCGG CATACGGGTT TTATATCCGG TCTGCCCCGC TGCTTTTGGC 351 GTGTCTGTTT TGCATGGGCG CGCAATCGAC GCTGTTCGGG CCGCTGAAAT 401 ACGCCATCCT GCCCGATTAT CTCGACGACA AAGAGTTGAT GATGGGCAAC 451 AGCCTGATTG AATCGGGTAC GTTTGTCGCC ATCCTGTTCG GTCAGATACT 501 GGGGACTGCG GTGGCAGGTG TACCGCCTTA TATTGTCGGG ATACTGGTTT 551 TGCTGGTCGC CGTAGGAGGC ACGGTCGGCA GCCTGTTTAT GCCGTCCGTA 601 CCCGCCAAGG CTGCCGATAC ACAAATTGAG TGGAATATTG TCCGGGGTAC 651 AAAATCCCTG CTGCGTGAAA CGGTGCGGCA CAAGCCCGTT TTTACCGCCA 701 TTATCGGTAT TTCGTGGTTT TGGTTTGTCG GCGCGGTTTA TACCACGCAA 751 CTGCCGACCT TTACCCAAAT CCATCTAGGC GGCAACGACA ATGTTTTCAA 801 CCTGATGCTT GCCCTGTTTT CCATCGGTAT TGCCGCCGGT TCGGTACTGT 851 GTGCCAAGTT CAGCAGGGAA CGGCTGAGGT TGGCTTGGGT AACGGTTGGT 901 GCGTTGGGTT TGACGGTTTG CGGCTTGGTT TTGGTGTGGC TGACGCACGG 951 ACACCGTTTT GAAGGGCTGA ACGGCATTTT TTGGTTTTTA TCGCAAGGAT 1001 GGGCATATCC CGTGATGGCG GTGATGACGC TGATCGGCTT TTTCGGCGGA 1051 TTTTTCTCCG TTCCGCTCTA TACCTGGCTG CAAACCGCCA GTAGCGAGAC 1101 TTTCCGCGCC CGCGCCGTTG CCGCCAACAA TATCGTTAAC GGTATTTTTA 1151 TGGTTTCCGC TGCCGTTTTG AGCGCGGTGT TGCTGTTTTT GTTTGACAGC 1201 ATTTCCTTGT TGTATCTGAT TGTCGCTTTG GGCAATATTC CGTTGTCGGT 1251 ATTTTTGATT AAGCGCGAAA GGCGGTTTTT AGGCGCGGCG GCAATCAGGA 1301 AAAAACCTTG A This corresponds to the amino acid sequence <SEQ ID 856; ORF 232. a>: a232.pep 1 MYAKKGGLGL VKSRRFAPLF ATQFLGAFΝD ΝVFKTALFVM IGFYGLGQΝG 51 FLPAGQMLΝL GALLFILPYF LFSSLSGQLG ΝKFDKAVLAR WAKVLEMIIM 101 AVAAYGFYIR SAPLLLACLF CMGAQSTLFG PLKYAILPDY LDDKELMMGΝ 151 SLIESGTFVA ILFGQILGTA VAGVPPYIVG ILVLLVAVGG TVGSLFMPSV 201 PAKAADTQIE WΝIVRGTKSL LRETVRHKPV FTAIIGISWF WFVGAVYTTQ 251 LPTFTQIHLG GΝDΝVFΝLML ALFSIGIAAG SVLCAKFSRE RLRLAWVTVG 301 ALGLTVCGLV LVWLTHGHRF EGLNGIFWFL SQGWAYPVMA VMTLIGFFGG 351 FFSVPLYTWL QTASSETFRA RAVAANNIVN GIFMVSAAVL SAVLLFLFDS 401 ISLLYLIVAL GNIPLSVFLI KRERRFLGAA AIRKKP* m232/a232 95.9% identity in 290 aa overlap 10 20 30 m232.pep MMGNSLIESGTFVAILFGQILGTAVAGVPP I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a232 ACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVAILFGQILGTAVAGVPP 120 130 140 150 160 170 40 50 60 70 80 90 m232.pep YIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTKSLLRETVRHKPVFTAIIG I I I I I II I I I I II I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a232 YIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTKΞLLRETVRHKPVFTAIIG 180 190 200 210 220 230 100 110 120 130 140 150 m232.pep ISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIGIAAGSVLCAKFSXERLMLAW I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I III III a232 ISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIGIAAGSVLCAKFSRERLRLAW 240 250 260 270 280 290 160 170 180 190 200 210 m232. pep VTVGALGLTVCGLVLVWLTHGHRFEGLNGIFXFLSQGWAYPVMAVMTLIGFFGGFFSVPL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I a232 VTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGWAYPVMAVMTLIGFFGGFFSVPL 300 310 320 330 340 350 220 230 240 250 260 m232. pep YT-VQTAIARFPRP-AVAANNIVNGIFMVSAAVLSAVLLFLFDSISLLYLIVALGNIPLS II : I I I :: I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a232 YTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSAVLLFLFDSISLLYLIVALGNIPLS 360 370 380 390 400 410 270 280 289 m232.pep VFLIKRERRFLGAAAIRKKPX I I I I I I I I II I I I I I I I I I I I a232 VFLIKRERRFLGAAAIRKKPX 420 430 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 857>: g233.seq 1 atgaaacgca aaaatatcgc gctgattccc gccgccggca tcggggtgcg 51 tttcggtgcg gacaaaccca agcaatatgt cgaaatcgga agcaaaaccg 101 ttttagaaca tgtacttggg atttttgaac ggcatgaggc cgtcgatttg 151 accgtcgttg tcgtctcgcc cgaagacacg tttgccgata aggttcagac 201 ggcatttcca caggttcggg tgtggaaaaa cggtggacag acccgcgccg 251 aaactgtccg caacggtgtg gcaaaactgt tggaaaccgg tttggcggcg 301 gaaaccgaca atattctggt acacgatgcc gcccgctgct gcctgccgtc 351 tgaagctctg gcgcggttga tagaacaggc gggcaacgcc gccgaaggcg 401 ggattttggc agttcccgtt gccgatacgc tcaagcgcgc agaaagcgga 451 caaatcagtg caactgtcga ccgttcgggg ctttggcagg cgcaaacgcc 501 gcagcttttt caagcgggtt tgctgcaccg cgcattggct gcggaaaact 551 tgggcggcat taccgatgaa gcgtccgccg tggaaaaact gggtgtgcgt 601 ccgctactga tacagggcga cgcgcgcaat ttgaaactga cgcagccgca 651 ggacgcatac atcgtcaggc tgctgctcaa tgccgtctga This corresponds to the amino acid sequence <SEQ ID 858; ORF 233.ng>: g233.pep 1 MKRKΝIALIP AAGIGVRFGA DKPKQYVEIG SKTVLEHVLG IFERHEAVDL 51 TWWSPEDT FADKVQTAFP QVRVWKΝGGQ TRAETVRΝGV AKLLETGLAA 101 ETDΝILVHDA ARCCLPSEAL ARLIEQAGΝA AEGGILAVPV ADTLKRAESG 151 QISATVDRSG LWQAQTPQLF QAGLLHRALA AENLGGITDE ASAVEKLGVR 201 PLLIQGDARN LKLTQPQDAY IVRLLLNAV* The following partial DNA sequence was identified in N meningitidis <SEQ ID 859>: m233.seq (partial) 1 ATGAAGCGCA AAAATATCGC GCTGATTCCC GCCGCCGGCA TCGGGGCGCG 51 TTTCGGTGCG GACAAACCCA AGCAATATGT CGAAATCGGA AGCAAAACCG 101 TTTTAGAACA TACGATTGGG ATTTTTGAAC GGCATGAGGC CGTCGATTTG 151 ACCGTCGTTG TCGTCTCGCC CGAAGACACG TTTGCCGATA AGGTTCAGAC 201 GGCATTTCCA CAGGTTCGGG TGTGGAAAAA CGGCGGACAG ACCCGCGCCG 251 AAACCGTCCG CAACGGTGTG GCAAAACTGT TGGAAACCGG TTTGGCGGCG 301 GAAACCGACA ATATTCTGGT ACACGATGCC GCGCGTTGCT GCCTGCCGTC 351 TGAAGCTTTG ACGCGGTTGA TAGAACAGGC GGGCAACGCC GCCGAAGGCG 401 GGATTTTGGC AATTCCCATT GCCGATACGC TCAAGTGCGC GGACGGTGGG 451 AACATT.... This corresponds to the amino acid sequence <SEQ ID 860; ORF 233>: m233.pep (partial) 1 MKRKΝIALIP AAGIGARFGA DKPKQYVEIG SKTVLEHTIG IFERHEAVDL 51 TWWSPEDT FADKVQTAFP QVRVWKΝGGQ TRAETVRΝGV AKLLETGLAA 101 ETDΝILVHDA ARCCLPSEAL TRLIEQAGΝA AEGGILAIPI ADTLKCADGG 151 ΝI.... Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N. gonorrhoeae ORF 233 shows 93.4% identity over a 152 aa overlap with a predicted ORF (ORF 233.ng) from N gonorrhoeae: m233/g233 10 20 30 40 50 60 m233.pep MKRKΝIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTIGIFERHEAVDLTVVVVSPEDT MIMMMMIMhMMMMMMMMMMhMMMMMMMMMMM g233 MKRKΝIALIPAAGIGVRFGADKPKQYVEIGSKTVLEHVLGIFERHEAVDLTWWSPEDT 10 20 30 40 50 60 70 80 90 100 110 120 m233. pep FADKVQTAFPQVRVWKΝGGQTRAETVRΝGVAKLLETGLAAETDΝILVHDAARCCLPSEAL M M I I I M M M M I M M M M M M M M M M M M M M M M M M M M M M g233 FADKVQTAFPQVRVWKΝGGQTRAETVRΝGVAKLLETGLAAETDΝILVHDAARCCLPSEAL 70 80 90 100 110 120 130 140 150 m233.pep TRLIEQAGΝAAEGGILAIPIADTLKCADGGΝI M I I I I I I I M I M I I h h l l l l l \ -- -- \ -- \ g233 ARLIEQAGΝAAEGGILAVPVADTLKRAESGQISATVDRSGLWQAQTPQLFQAGLLHRALA 130 140 150 160 170 180 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 861>: a233. seq 1 ATGAAGCGCA AAAATATCGC GCTGATTCCC GCCGCCGGCA TCGGGGCGCG 51 TTTCGGTGCG GACAAACCCA AGCAATATGT CGAAATCGGA AGCAAAACCG 101 TTTTAGAACA TACGATTGGG ATTTTTGAAC GGCATGAGGC CGTCGATTTG 151 ACCGTCGTTG TCGTCTCGCC CGAAGACACG TTTGCCGATA AGGTTCAGAC 201 GGCATTTCCA CAGGTTCGGG TGTGGAAAAA CGGCGGACAG ACCCGCGCCG 251 AAACTGTCCG CAACGGTGTG GCAAAATTGT TGGAAACCGG TTTGGCGGCG 301 GAAACCGACA ATATTCTGGT ACACGATGCC GCGCGTTGCT GCCTGCCGTC 351 TGAAGCTTTG ACGCGGTTGA TAGAACAGGC GGGCAACGCT GCCGAAGGTG 401 GGATTTTGGC AATTCCCGTT GCCGATACGC TCAAGTGCGC GGACGGTGGG 451 AACATTAGTG CAACCGTCGA GCGGACGAGC CTTTGGCAGG CGCAAACGCC 501 GCAGCTTTTC CGCGCCGGGC TGCTGCACCG CGCATTGGCT GCGGAAAACT 551 TGGACGGCAT TACCGATGAA GCGTCCGCCG TGGAAAAATT GGGCATCCGC 601 CCTTTGCTGG TGCAGGGCGA CGCGCGCAAT TTGAAACTGA CGCAGCCGCA 651 GGACGCATAC ATCGTCAGGC TGCTGCTCGA TGCCGTCTGA This corresponds to the amino acid sequence <SEQ ID 862; ORF 233. a>: a233.pep 1 MKRKNIALIP AAGIGARFGA DKPKQYVEIG SKTVLEHTIG IFERHEAVDL 51 TWWSPEDT FADKVQTAFP QVRVWKNGGQ TRAETVRNGV AKLLETGLAA 101 ETDNILVHDA ARCCLPSEAL TRLIEQAGNA AEGGILAIPV ADTLKCADGG 151 NISATVERTS LWQAQTPQLF RAGLLHRALA AENLDGITDE ASAVEKLGIR 201 PLLVQGDARN LKLTQPQDAY IVRLLLDAV* m233/a233 99.3% identity in 152 aa overlap 10 20 30 40 50 60 m233. pep MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTIGIFERHEAVDLTVWVSPEDT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a233 MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTIGIFERHEAVDLTWWSPEDT 10 20 30 40 50 60 70 80 90 100 110 120 m233. pep FADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLETGLAAETDNILVHDAARCCLPSEAL I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a233 FADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLETGLAAETDNILVHDAARCCLPSEAL 70 80 90 100 110 120 130 140 150 m233.pep TRLIEQAGNAAEGGILAIPIADTLKCADGGNI I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I a233 TRLIEQAGNAAEGGILAIPVADTLKCADGGNISATVERTSLWQAQTPQLFRAGLLHRALA 130 140 150 160 170 180 a233 AENLDGITDEASAVEKLGIRPLLVQGDARNLKLTQPQDAYIVRLLLDAVX 190 200 210 220 230 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 863>: g234.seq 1 atgaaaaccg tttccgccgc catcgctttt gccgccgctg ccgtttcact 51 gaccggctgt gcgaccgagt cctcacgcag cctcgaggtt gcaaaagtcg 101 cctcctgcaa tacgcaatat cacggtgttc gcaccccgat ttccgtcgga 151 acattcgaca accgctccag cttccaaaaa ggcattttct ccgacagtga 201 agaccgtctg ggcagccagg caaaaaccat cctggtaaca cacctgcaac 251 aaaccaaccg cttcaacgta ctgaaccgca ccaaccttag cgcattgaaa 301 caggaatccg gcatttccgg caaagcgcag aacctgaaag gcgcagatta 351 tgtcgttacc ggcgatgtaa ccgaattcgg acgcagagat gtcggcgatc 401 atcagctctt cggcattttg ggtcgcggca aatcgcaaat cgcctatgca 451 aaagtggctc tgaatatcgt caacgtcaat acttccgaaa tcgtctattc 501 cacacagggc gcgggcgaat acgcactttc caaccgcgaa atcatcggtt 551 tcggcggcac ttccggctac gatgcgactt tgaacggcaa agttttagac 601 ttggcaatcc gcgaagccgt cgacaacttg gttcaggctg tcgacaacgg 651 cgcatggcaa tccaaccgtt aa This corresponds to the amino acid sequence <SEQ ID 864; ORF 234.ng>: g234.pep 1 MKTVSAAIAF AAAAVSLTGC ATESSRSLEV AKVASCΝTQY HGVRTPISVG 51 TFDΝRSSFQK GIFSDSEDRL GSQAKTILVT HLQQTΝRFΝV LΝRTΝLSALK 101 QESGISGKAQ ΝLKGADYWT GDVTEFGRRD VGDHQLFGIL GRGKSQIAYA 151 KVALΝIVΝVΝ TSEIVYSTQG AGEYALSΝRE IIGFGGTSGY DATLΝGKVLD 201 LAIREAVDΝL VQAVDΝGAWQ SΝR* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 865>: m234.seq (partial) 1 ...GGCGCGGGCG AATACGCACT TTCCAACCGt GAAATCATCG GTTTCGGCGG 51 CACTTCCGGC TACGATGCGA CTTTGAACGG CAAAGTTTTA GACTTGGCAA 101 TCCGCGAAGC .gTCAACAGC CTGGTTCAGG CTGTTGACAA CGGCGCATGG 151 CAACCCAACC GTTAA This corresponds to the amino acid sequence <SEQ ID 866; ORF 234>: m234 . pep (partial ) 1 ..GAGEYALSNR EIIGFGGTSG YDATLNGKVL DLAIREAVNS LVQAVDNGAW 51 QPNR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 234 shows 94.4% identity over a 54 aa overlap with a predicted ORF (ORF 234.ng) from N gonorrhoeae: m234/g234 10 20 30 m234.pep GAGEYALSΝREIIGFGGTSGYDATLΝGKVL MMMMIMMMMMMMIMMM g234 LGRGKSQIAYAKVALΝIVΝVΝTSEIVYSTQGAGEYALSΝREIIGFGGTSGYDATLΝGKVL 140 150 160 170 180 190 40 50 m234.pep DLAIREAVΝSLVQAVDΝGAWQPΝRX M M M I h M M M M M M I I I g234 DLAIREAVDΝLVQAVDΝGAWQSΝRX 200 210 220 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 867>: a234.seq (partial) 1 AACCGCACCT ATTTGAACGC ATTAAAACAG GAATCCGGCA TTTCCGGCAA 51 AGCGCATAAC CTGAAAGGCG CAAATTATGT CGΝΝACCGGC GATGTAACCG 101 AATTCGGACG CAΝAGATGTC GGCGATCATC AGCTCTTCGG CATTTTGGGT 151 CGCGGCAAAT CGCAAATCGC CTATGCAAAA GTGGCTCTGA ATATCGTCAA 201 CGTCAATACT TCCGAAATCG TCTATTCCGC ACAGGGCGCG GGCGAATACG 251 CACTTTCCAA CCGTGAAATC ATCGGTTTCG GCGGCACTTC CGGCTACGAT 301 GCGACTTTGA ACGGCAAAGT TTTAGACTTG GCAATCCGCG AAGCCGTCAA 351 CAGCCTGGTT CAGGCTGTTG ACAACGGCGC ATGGCAACCC AACCGTTAA This corresponds to the amino acid sequence <SEQ ID 868; ORF 234.a>: a234.pep (partial) 1 ΝRTYLΝALKQ ESGISGKAHΝ LKGAΝYVXTG DVTEFGRXDV GDHQLFGILG 51 RGKSQIAYAK VALΝIVΝVΝT SEIVYSAQGA GEYALSΝREI IGFGGTSGYD 101 ATLΝGKVLDL AIREAVΝSLV QAVDΝGAWQP ΝR* m234/a234 100.0% identity in 54 aa overlap 10 20 30 m234 . pep GAGEYALSΝREI IGFGGTSGYDATLΝGKVL a234 LGRGKSQIAYAKVALΝIVΝVΝTSEIVYSAQGAGEYALSΝREIIGFGGTSGYDATLΝGKVL 50 60 70 80 90 100 40 50 m234 . pep DLAIREAVΝSLVQAVDΝGAWQPΝRX I I I I I I I I I I I I I I I I I I I I I I I I I a234 DLAIREAVΝSLVQAVDΝGAWQPΝRX 110 120 130 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 869>: g235 . seq 1 atgaaacctt tgattttagg gcttgccgcc gtgttggctc tgtctgcctg 51 ccaagttcga aaagctcccg acctcgacta cacgtcattc aaagaaagca 101 aaccggcttc aattttggtg gttccgccgc tgaacgagtc gcctgatgtc 151 aacggcactt gggggatgct ggcttcgacc gccgcgccga tttccgaagc 201 cggctattac gtctttcccg ccgcagtcgt ggaggaaacc ttcaaagaaa 251 acggcttgac caatgccgcc gatattcacg ccgtccggcc ggaaaaactg 301 catcaaattt tcggcaatga tgcggttttg tacattacgg ttaccgaata 351 cggcacttca tatcaaattt tagacagcgt gacgaccgta tccgccaaag 401 cacggctggt cgattcccgc aacgggaaag agttgtggtc gggttcggcc 451 agcatccgcg aaggcagcaa caacagcaac agcggcctgt tgggggcttt 501 ggtcggcgca gtggtcaatc agattgccaa cagcctgacc gaccgcggtt 551 atcaggtttc caaaaccgcc gcatacaacc tactgtcgcc ctattcccgc 601 aacggtatct tgaaaggtcc gagattcgtc gaagagcagc ccaaataa This corresponds to the amino acid sequence <SEQ ID 870; ORF 235.ng>: g235.pep 1 MKPLILGLAA VLALSACQVR KAPDLDYTSF KESKPASILV VPPLNESPDV 51 NGTWGMLAST AAPISEAGYY VFPAAWEET FKENGLTNAA DIHAVRPEKL 101 HQIFGNDAVL YITVTEYGTS YQILDSVTTV SAKARLVDSR NGKELWSGSA 151 SIREGSNNSN SGLLGALVGA WNQIANSLT DRGYQVSKTA AYNLLSPYSR 201 NGILKGPRFV EEQPK* The following partial DNA sequence was identified in N meningitidis <SEQ ID 871>: m235. seq 1 ATGAAACCTT TGATTTTAGG GCTTGCCGCC GTGTTGGCGC TGTCTGCCTG 51 CCAAGTTCAA AAAGCGCCCG ATTTCGACTA CACGTCATTC AAGGAAAGCA 101 AACCGGCTTC AATTTTGGTG GTTCCGCCGC TGAACGAATC GCCCGATGTC 151 AACGGAACAT GGGGTGTACT GGCTTCGACC GCCGCGCCGC TTTCCGAAGC 201 CGGCTATTAC GTCTTCCCCG CCGCAGTCGT GGAGGAAACC TTCAAACAAA 251 ACGGCTTGAC CAATGCCGCC GATATTCACG CCGTCCGGCC GGAAAAACTG 301 CATCAGATTT TCGGCAATGA TGCGGTTTTG TACATTACGG TTACCGAATA 351 CGGCACTTCA TATCAAATTT TAGACAGCGT GACGACCGTA TCCGCCAAAG 401 CACGGCTGGT CGATTCCCGC AACGGAAAAG AGTTGTGGTC GGGTTCGGCC 451 AGCATCCGCG AAGGCAGCAA CAACAGCAAC AGCGGCCTGT TGGGGGCTTT 501 GGTCAGCGCA GTGGTCAATC AGATTGCCAA CAGCCTGACC GACCGCGGTT 551 ATCAGGTTTC CAAAACCGCC GCATACAACC TGCTGTCGCC CTATTCTCAC 601 AACGGCATCT TGAAAGGTCC GAGATTCGTT GAAGAGCAGC CCAAATAA This corresponds to the amino acid sequence <SEQ ID 872; ORF 235>: m235. pep 1 MKPLILGLAA VLALSACQVQ KAPDFDYTSF KESKPASILV VPPLNESPDV 51 NGTWGVLAST AAPLSEAGYY VFPAAWEET FKQNGLTNAA DIHAVRPEKL 101 HQIFGNDAVL YITVTEYGTS YQILDSVTTV SAKARLVDSR NGKELWSGSA 151 SIREGSNNSN SGLLGALVSA WNQIANSLT DRGYQVSKTA AYNLLSPYSH 201 NGILKGPRFV EEQPK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 235 shows 96.7% identity over a 215 aa overlap with a predicted ORF (ORF 235.ng) from N gonorrhoeae: m235/g235 10 20 30 40 50 60 m235.pep MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILWPPLΝESPDVΝGTWGVLAST g235 MKPLILGLAAVLALSACQVRKAPDLDYTSFKESKPASILWPPLΝESPDVΝGTWGMLAST 10 20 30 40 50 60 70 80 90 100 110 120 m235.pep AAPLSEAGYYVFPAAWEETFKQΝGLTΝAADIHAVRPEKLHQIFGΝDAVLYITVTEYGTS g235 AAPISEAGYYVFPAAWEETFKEΝGLTΝAADIHAVRPEKLHQIFGΝDAVLYITVTEYGTS 70 80 90 100 110 120 130 140 150 160 170 180 m235.pep YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNSGLLGALVSAWNQIANSLT g235 YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNSGLLGALVGAWNQIANSLT 130 140 150 160 170 180 190 200 210 m235. pep DRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPKX g235 DRGYQVSKTAAYNLLSPYSRNGILKGPRFVEEQPKX 190 200 210 The following partial DNA sequence was identified in N meningitidis <SEQ ID 873>: a235. seq 1 ATGAAACCTT TGATTTTAGG GCTTGCCGCC GTGTTGGCGC TGTCTGCCTG 51 CCAAGTTCAA AAAGCGCCCG ATTTCGACTA CACGTCATTC AAGGAAAGCA 101 AACCGGCTTC AATTTTGGTG GTTCCGCCGC TGAACGAATC GCCCGATGTC 151 AACGGAACAT GGGGTGTACT GGCTTCGACC GCCGCGCCGC TTTCCGAAGC 201 CGGCTATTAC GTCTTCCCCG CCGCAGTCGT GGAGGAAACC TTCAAACAAA 251 ACGGCTTGAC CAATGCCGCC GATATTCACG CCGTCCGGCC GGAAAAACTG 301 CATCAGATTT TCGGCAATGA TGCGGTTTTG TACATTACGG TTACCGAATA 351 CGGCACTTCA TATCAAATTT TAGACAGCGT GACGACCGTA TCCGCCAAAG 401 CACGGCTGGT CGATTCCCGC AACGGAAAAG AGTTGTGGTC GGGTTCGGCC 451 AGCATCCGCG AAGGCAGCAA CAACAGCAAC AGCGGCCTGT TGGGGGCTTT 501 GGTCAGCGCA GTGGTCAATC AGATTGCCAA CAGCCTGACC GACCGCGGTT 551 ATCAGGTTTC TAAAACCGCC GCATACAACC TGCTGTCGCC CTATTCTCAC 601 AACGGCATCT TGAAAGGTCC GAGATTCGTC GAAGAGCAGC CCAAATAA This corresponds to the amino acid sequence <SEQ ID 874; ORF 235. a>: a235.pep 1 MKPLILGLAA VLALSACQVQ KAPDFDYTSF KESKPASILV VPPLNESPDV 51 NGTWGVLAST AAPLSEAGYY VFPAAWEET FKQNGLTNAA DIHAVRPEKL 101 HQIFGNDAVL YITVTEYGTS YQILDSVTTV SAKARLVDSR NGKELWSGSA 151 SIREGSNNSN SGLLGALVSA WNQIANSLT DRGYQVSKTA AYNLLSPYSH 201 NGILKGPRFV EEQPK* m235/a235 100.0% identity in 215 aa overlap 10 20 30 40 50 60 m235.pep MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILWPPLNESPDVNGTWGVLAST I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I a235 MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILWPPLNESPDVNGTWGVLAST 10 20 30 40 50 60 70 80 90 100 110 120 m235. pep AAPLSEAGYYVFPAAWEETFKQNGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I a235 AAPLSEAGYYVFPAAWEETFKQNGLTNAADIHAVRPEKLHQIFGNDAVLYITVTEYGTS 70 80 90 100 110 120 130 140 150 160 170 180 m235 . pep YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNSGLLGALVSAWNQIANSLT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a235 YQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSNSGLLGALVSAWNQIANSLT 130 140 150 160 170 180 190 200 210 m235. pep DRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPKX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a235 DRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPKX 190 200 210 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 875>: g236 . seq 1 ATGGCGCGTT TCGCCTTCTC CGCCGACATT CTCCGCACAG CGTTTGCAGA 51 CGGTTTCATA ACCTGCAACC GCGCCCACAT CGCGGGTGTA ATGCCAGCAG 101 CGTTCGCATT TTTCGCCGTC GCTGGCTTTG GCGGCAACGG CAAGTTCATC 151 ACCGACTTTC ACTTCTGCTT TAGACACCAG CAGGGCAAAG CGCAATTCTT 201 CGCCCAAAGC ATTCAGATAG CCGGCCATTT CTTCCGGCGC GGTAATTTCG 251 GCTTCCGCCT GCAAggacga accgacagTT TTGTcggcGC GCAAAGGCTC 301 GAtagcggcg gTTACTGCTT CGCGCGCTTC GCGGATTGCC GTCCATTTTT 351 TCACCAGTTC GGCTTCGGCT TTTTCGTTGA TGGCCGGGAA CTCGTGCCAA 401 GTATGGAAGA GGACGCTGTC TTCTTCGCCG CCGCCGATGA TGTCCCACGC 451 TTCTTCGCCG GTGAAGCACA AAATCGGTGC AATCAAGAGA ACCAGGCTGC 501 GCGTGATGTG GTACAGGGCG GTTTGCGCGC TGCGGCGGGC GCGGCTGTCG 551 GCTTTGGTGG TGTAGAGGCG GTCTTTCAGG ATGTCGAGGT AGAACGCGCC 601 CAAGTCTTCC GAGCAGAAAG AAACAATGTC TTTCACGGCG AAGTGGAAGG 651 CATAGCGCGG ATAGTAACCG CCTGCCAAAC GCTCTTGCAG CCGCCGCGCC 701 AATACCAAGG CGTAGCGGTC GATTTCCACC ATATCCGCCT GTTGCACGGC 751 ATCTTCAATC GGATTAAAGT CGCTCAAATT GGCAAAcagG AAGCTCAAGG 801 TATTGCGGAT GCGGCGGTAG CTTTCGGTAA CGCGTTTGAG GATTTCTTTG 851 GAAatcgCCA ATtcgccgct gTAATCGGTG GATGCCGCCC ACAGGCGCAG 901 GATGTCCGCG CCGAATTCGT TATAGACTTC CTGCGGCGCG ACGACGTTGC 951 CGATGGATTT CGACATTTTG CGGCCGTTTT GGTCAACCAC GAAACCGTGG 1001 GTCAGCAGCT GTTTATACGG TGCGCGTCCC ATGGATGA This corresponds to the amino acid sequence <SEQ ID 876; ORF 236.ng>: g236.pep 1 MARFAFSADI LRTAFADGFI TCNRAHIAGV MPAAFAFFAV AGFGGNGKFI 51 TDFHFCFRHQ QGKAQFFAQS IQIAGHFFRR GNFGFRLQGR TDSFVGAQRL 101 DSGGYCFARF ADCRPFFHQF GFGFFVDGRE LVPSMEEDAV FFAAADDVPR 151 FFAGEAQNRC NQENQAARDV VQGGLRAAAG AAVGFGGVEA VFQDVEVERA 201 QVFRAERNNV FHGEVEGIAR IVTACQTLLQ PPRQYQGVAV DFHHIRLLHG 251 IFNRIKVAQI GKQEAQGIAD AAVAFGNAFE DFFGNRQFAA VIGGCRPQAQ 301 DVRAEFVIDF LRRDDVADGF RHFAAVLVNH ETVGQQLFIR CASHG* The following partial DNA sequence was identified in N meningitidis <SEQ ID 877>: m236.seq (partial) 1 .. TTGCACGGAC GAACCGACGG TTTTGTCGGC GCGCAAAGGC TCGATGGCGG 51 CGGTTACCGC TTCGCGGGCT TCGCGGATTG CCGTCCATTT TTTCACCAGT 101 TCGGCTTCGG TTTTTTCGTT GATGGTCGGG AACTCGTGCC AAGTATGGAA 151 GAGGACGCTG TCkTCTTCGC CGCCGCCGwT GAyGTCCCAC GCTTCTTCGC 201 CGGTGAAGCA CAAAATCGGT GCAATCAAGA GAACCAAACT GCGTGTGATG 251 TGATACAGGG CAGTTTGTGC GCTGCGGCGT GCATGGCTGT CTGCTTTGGT 301 GGTGTAGAGG CGGTCTTTCA GGATGTCGAG GTAGAACGCA CCCAAGTCTT 351 CCGAGCAGAA AGAAACArTG TCTTTTACGG CAAAGTGGaA kGCATAACGC 401 GGATAGTAAT CGCCTGCCAG ACACTCTTGC AGCTGACGTG CCAATACCAC 451 GGCGTAGCGG TCGATTTCCA CCATATCCGC CTGTTGCACG GCATCTTCAA 501 TCGGATTAAA GTCGCTCAAG TTGGCAAACA AAAAGCTCAA GGTATTGCGG 551 ATACGGCGGT AgCTTTCGGT TACGCGTTTG AGGATTTCTT TGGAAATCGC 601 CAATTCGCCG CTGTAATCGG TAGATGCCGC CCACAGGCGC AGGATGTCTG 651 CGCCGAATTC GTTATAAACC TCTTGCGGTG CAACGACGTT GCCGATGGAT 701 TTCGACATTT TTTTGCCTTC GCCGTCGACA ACGAAACCAT GGGTCAGCAG 751 CTGTTTATAC GGCGCGCGAC CCATTGA This corresponds to the amino acid sequence <SEQ ID 878; ORF 236>: m236.pep (partial) 1 ..LHGRTDGFVG AQRLDGGGYR FAGFADCRPF FHQFGFGFFV DGRELVPSME 51 EDAVXFAAAX DVPRFFAGEA QΝRCΝQEΝQT ACDVIQGSLC AAACMAVCFG 101 GVEAVFQDVE VERTQVFRAE RΝXVFYGKVE XITRIVIACQ TLLQLTCQYH 151 GVAVDFHHIR LLHGIFΝRIK VAQVGKQKAQ GIADTAVAFG YAFEDFFGΝR 201 QFAAVIGRCR PQAQDVCAEF VIΝLLRCΝDV ADGFRHFFAF AVDΝETMGQQ 251 LFIRRATH* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 236 shows 82.9% identity over a 258 aa overlap with a predicted ORF (ORF 236.ng) from N gonorrhoeae: m236/g236 10 20 30 m236. pep LHGRTDGFVGAQRLDGGGYRFAGFADCRPF II g236 FRHQQGKAQFFAQSIQIAGHFFRRGNFGFRLQGRTDSFVGAQRLDSGGYCFARFADCRPF 60 70 80 90 100 110 40 50 60 70 80 90 m236. pep FHQFGFGFFVDGRELVPSMEEDAVXFAAAXDVPRFFAGEAQNRCNQENQTACDVIQGSLC MMMMMMMMMMMM MM 111 II II 1111 II II I II h I Ihlhl g236 FHQFGFGFFVDGRELVPSMEEDAVFFAAADDVPRFFAGEAQNRCNQENQAARDWQGGLR 120 130 140 150 160 170 100 110 120 130 140 150 m236. pep AAACMAVCFGGVEAVFQDVEVERTQVFRAERNXVFYGKVEXITRIVIACQTLLQLTCQYH III II MMMMMIMIhllMMM MM hill Ih g236 AAAGAAVGFGGVEAVFQDVEVERAQVFRAERNNVFHGEVEGIARIVTACQTLLQPPRQYQ 180 190 200 210 220 230 160 170 180 190 200 210 m236.pep GVAVDFHHIRLLHGIFNRIKVAQVGKQKAQGIADTAVAFGYAFEDFFGNRQFAAVIGRCR MMMMMMMM II g236 GVAVDFHHIRLLHGIFNRIKVAQIGKQEAQGIADAAVAFGNAFEDFFGNRQFAAVIGGCR 240 250 260 270 280 290 220 230 240 250 259 m236. pep PQAQDVCAEFVINLLRCNDVADGFRHFFAFAVDNETMGQQLFIRRATHX IIMhMI MMIIIIM I hMhlMMM hi g236 PQAQDVRAEFVIDFLRRDDVADGFRHFAAVLVNHETVGQQLFIRCASHG 300 310 320 330 340 The following partial DNA sequence was identified in N meningitidis <SEQ ID 879>: a236. seq 1 ATGGCGCGTT TCGCCTTCTC CGCCGACATT CTCTGCACAG CGTTTGCAGA 51 CGGTTTCATG GCCTGCAACC GCGCCCACAT CGCGGGTGTA GTGCCAGCAG 101 CGTTCGCATT TTTCACCATC ACTGGCTTTA GCGGCAACGG CAAGTTCGCT 151 GCCTACTTTC ACTTCTGCTT TAGACACCAG CAAAGCAAAG CGCAATTCTT 201 CGCCCAAAGC ATTCAGATAG CCGGCCATTT CTTCCGGCGC GGTAATTTCG 251 GCTTCGGCTT GCAAGGACGA ACCGACGGTT TTGTCGGCGC GCAAAGGCTC 301 GATGGCGGCG GTTACCGCTT CGCGGGCTTC GCGGATTGCC GTCCATTTTT 351 TCACCAGTTC GGCTTCGGCT TTTTCGTTGA TGGTCGGGAA CTCGTGCCAA 401 GTATGGAAAA GCACGCTGTC TTCTGCGCCG CCGCCGATGA TGTCCCACGC 451 TTCTTCGCCG GTGAAGCACA AAATCGGTGC AATCAAGAGA ACCAGGCTGC 501 GCGTGATGTG GTACAGGGCG GTTTGCGCGC TGCGGCGGGC GCGGCTGTCG 551 GCTTTGGTGG TATAGAGGCG GTCTTTCAGG ATATCGAGGT AGAACGCGCC 601 CAAGTCTTCC GAGCAGAAAG AAACCATTTC TTTCACGGCA AAGTGGAAGG 651 CATAACGCGG ATAAAAATCA CCGGCAACGC GTTCTTGCAG CCGCCTTGCC 701 AACACCAAGG CATAGCGGTC GATTTCCACC ATATCCGCCT GTTGCACGGC 751 ATCTTCAATA GGATTGAAGT CGCTCAAGTT GGCAAACAAA AAGCTCAAGG 801 TATTGCGGAT ACGGCGGTAG CTTTCGGTTA CGCGCTTGAG GATTTCTTTG 851 GAAATCGCCA ATTCGCCGCT GTAATCGGTG GATGCCGCCC ACAGGCGCAG 901 GATGTCCGCG CCGAACTCGT TATACACTTC TTGCGGCGCG ACGACGTTGC 951 CGATGGATTT CGACATTTTG CGCCCGTTTT GATCCACCAC GAAACCATGG 1001 GTCAGCAGCT GTTTGTACGG CGCGCGACCC ATTGA This corresponds to the amino acid sequence <SEQ ID 880; ORF 236.a>: a236.pep 1 MARFAFSADI LCTAFADGFM ACΝRAHIAGV VPAAFAFFTI TGFSGΝGKFA 51 AYFHFCFRHQ QSKAQFFAQS IQIAGHFFRR GΝFGFGLQGR TDGFVGAQRL 101 DGGGYRFAGF ADCRPFFHQF GFGFFVDGRE LVPSMEKHAV FCAAADDVPR 151 FFAGEAQNRC NQENQAARDV VQGGLRAAAG AAVGFGGIEA VFQDIEVERA 201 QVFRAERNHF FHGKVEGITR IKITGNAFLQ PPCQHQGIAV DFHHIRLLHG 251 IFNRIEVAQV GKQKAQGIAD TAVAFGYALE DFFGNRQFAA VIGGCRPQAQ 301 DVRAELVIHF LRRDDVADGF RHFAPVLIHH ETMGQQLFVR RATH* m236/a236 81.0% identity in 258 aa overlap 10 20 30 m236. pep LHGRTDGFVGAQRLDGGGYRFAGFADCRPF I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I a236 FRHQQSKAQFFAQSIQIAGHFFRRGNFGFGLQGRTDGFVGAQRLDGGGYRFAGFADCRPF 60 70 80 90 100 110 40 50 60 70 80 90 m236. pep FHQFGFGFFVDGRELVPSMEEDAVXFAAAXDVPRFFAGEAQNRCNQENQTACDVIQGSLC I I I I I I I I I I I I I I I I I I I I : II III I I I I I I II I I I I I I I I I I I : I I I : I I a236 FHQFGFGFFVDGRELVPSMEKHAVFCAAADDVPRFFAGEAQNRCNQENQAARDWQGGLR 120 130 140 150 160 170 100 110 120 130 140 150 m236. pep AAACMAVCFGGVEAVFQDVEVERTQVFRAERNXVFYGKVEXITRIVIACQTLLQLTCQYH III II I I I : I I I I I I : I I I I : I I I I I I I I llll I I I I I: ::: I I II:: a236 AAAGAAVGFGGIEAVFQDIEVERAQVFRAERNHFFHGKVEGITRIKITGNAFLQPPCQHQ 180 190 200 210 220 230 160 170 180 190 200 210 m236. pep GVAVDFHHIRLLHGIFNRIKVAQVGKQKAQGIADTAVAFGYAFEDFFGNRQFAAVIGRCR I : I I I I I I I I I I I I I I I I I: I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I II a236 GIAVDFHHIRLLHGIFNRIEVAQVGKQKAQGIADTAVAFGYALEDFFGNRQFAAVIGGCR 240 250 260 270 280 290 220 230 240 250 259 m236. pep PQAQDVCAEFVINLLRCNDVADGFRHFFAFAVDNETMGQQLFIRRATHX llllll I I : I I:: I I : I I I I I I I I I : : I I I I I I I I : I I I I I I a236 PQAQDVRAELVIHFLRRDDVADGFRHFAPVLIHHETMGQQLFVRRATHX 300 310 320 330 340 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 881>: g237.seq 1 atgcgggaca aggttggcgg taatatcgca ctccccgccc cacgaatatt 51 cgattctaac atcggcaagc tgcggaaaaa ctttaagcat atcttggcgg 101 acaagctcgg tcatacgcgc aggattgtcg ataaattcgt tatccttacc 151 gccgaaaagc agcctgccgt ccgcgctgag gcggtaataa tccaaaatat 201 ggcggttgtc gcatactgcc atattgttgc ggataagccc ttttgtgcgc 251 gcgcccaagg gttcggtggc aataataaag gtgctgacgg caatcgcctt 301 gcgttccaaa ggccggaata tcgggttcaa accgacataa gtattgacgg 351 catagaccac atttttacac tcgacgctgc cttcgggcgt gtaaaccagc 401 caaccgtttt gatacggttc gatgcgcgtc atcggggatt gctcgaaaat 451 ctgcgcgccg gcttcggcag cggcgctggc aacacccaac gtgtaattga 501 gcggatgaag atgcccggac aagggatcga actgtgcgcc ttggtacata 551 tcgctgtcaa gctgctgttt caactcggct ttatcccaaa gttgataatg 601 actcgcaccg taatgccgtt gggcgtgttc atgccactgc tgcaactctt 651 cccaatgctg cggacggacg gcaaccgtgg cataaccgcg ctgccaatcg 701 caatcgatgg catgtttgcg gacgcgttcg tccaccagtt cgaccgcctg 751 caaagactgt tgccaaaacc attgcgcctg ctccaagccg acctgttttt 801 caatttcccc cataccgcag gcgtagtcgc tgataacctg cccgccactc 851 ctgccggacg cgccgaagcc gatacgtgcg gcttccaaaa cgacggcttc 901 atgtccgtgt tccgccagcg gcaatgcggt acacaaaccg ctcaaaccgc 951 cgccgataat gcaggtttcg gctttcagac ggcattggag tttcggataa 1001 acagtatgcg gattaaccga actaaaataa taagaaggca gatattcttg 1051 aaaatcaggg cgaatcattg tgtttgcttt atcgggtata ttttcggacg 1101 gaatgataca gactgtcggg ccatatcgtc caaacagaaa atcggttga This corresponds to the amino acid sequence <SEQ ID 882; ORF 237.ng>: g237.pep 1 MRDKVGGΝIA LPAPRIFDSΝ IGKLRKΝFKH ILADKLGHTR RIVDKFVILT 51 AEKQPAVRAE AVIIQΝMAW AYCHIVADKP FCARAQGFGG ΝΝKGADGΝRL 101 AFQRPEYRVQ TDISIDGIDH IFTLDAAFGR VNQPTVLIRF DARHRGLLEN 151 LRAGFGSGAG NTQRVIERMK MPGQGIELCA LVHIAVKLLF QLGFIPKLIM 201 TRTVMPLGVF MPLLQLFPML RTDGNRGITA LPIAIDGMFA DAFVHQFDRL 251 QRLLPKPLRL LQADLFFNFP HTAG ADNL PATPAGRAEA DTCGFQNDGF 301 MSVFRQRQCG TQTAQTAADN AGFGFQTALE FRINSMRINR TKIIRRQIFL 351 KIRANHCVCF IGYIFGRNDT DCRAISSKQK IG* The following partial DNA sequence was identified in N meningitidis <SEQ TD 883>: m237.seq 1 ATGCGGGACA AGGTTGGCGG TAATGTCGCA CTCCCCGCCC CACGAATATT 51 CGATTTTGAC ATCGGCAAGC TGCGGAAAAA CTTTAAGCAT ATCTTGGCGG 101 ACAAGCTCGG TCATaCGCTC AGGATTGTCG ATAAACTCGT TATCCTTACC 151 GCCGAAAAGC AGTCTGCCGT CCGCGCTGAG GCGGTAATAA TCCAAAATAT 201 GGCGGTTGTC GCATACTGCC ATATTGTTAC GGATAAGCCC TTTTGCGCGC 251 GCCCCCAAGG GTTCGGTCGC AATAATAAAG GTGCTGACAG CAATCGCCTT 301 GCGTTCCAAA GGCCGGAATA TCGGGTTCAA ACCTGCATAA GTATTGACAG 351 CATAGACCAC ATTTTTGCAC TCGACGCTGC CTTCGGGCGT GTAAACCAGC 401 CAACCGTTTT GATGCGGTTC GATGCACGTC ATCGGGGATT GCTCGAAAAT 451 CTGCGCACCG GCTTCGGCAG CGGCACGAGC GATGCCCAAA GTGTAAGTGA 501 GCGGATGCAG GTGTCCGGAT AAGGGGTCGA ATTGTGCCCC TTGGTACATA 551 TCGCTGTCAA GCTGCTGTTT CAACTCGGCT TTATCCCAAA GTTGATAATG 601 ACTCGCACCG TAATGCCGTT GGGCGTGTTC ATGCCACTGC TGCAACTCTT 651 CCCAATGCTG CGGACGGACG GCAACCGTGG CATAACCGCG CTGCCAATCA 701 CAATCGACGG CATGTTTGCG GACGCGTTCG TCCACCAGTT CGACCGCCTG 751 CAAAGACTGT TGCCAAAACC ATTGCGCCTG CTCCAAGCCG ACCTGTTTTT 801 CAATTTCCCC CATACCGCAG nCGTAATCGC TGATAACCTG CCCGCCACTC 851 CGTCCCGACG CGCCGAAACC GATACGCGCG GCTTCCAACA CAACCGTTTC 901 ATGTCCCTGC TCCGCCAAGG GCAATGCAGT GCACAAACCA CCCAATCCGC 951 CGCCGATGAT ACAGGTATCG GTTTTCAGAC GGCATTGAAG TTtCGGATAA 1001 ACAGTATGAG GATTAACCGA ACTGAAATAA TAAGAAGGCA GATATTCTTG 1051 AAAATCAGGG CGAATCATTG TGTTTGCTTT ATCAGGTGTA TTTTCGGACG 1101 GAATGATACA GGCTGTCGGG CCATATCGTC CA ACAGAAA ATCGGTTGA This corresponds to the amino acid sequence <SEQ ED 884; ORF 237>: m237.pep 1 MRDKVGGΝVA LPAPRIFDFD IGKLRKΝFKH ILADKLGHTL RIVDKLVILT 51 AEKQSAVRAE AVIIQΝMAW AYCHIVTDKP FCARPQGFGR ΝΝKGADSΝRL 101 AFQRPEYRVQ TCISIDSIDH IFALDAAFGR VΝQPTVLMRF DARHRGLLEN 151 LRTGFGSGTS DAQSVSERMQ VSGXGVELCP LVHIAVKLLF QLGFIPKLIM 201 TRTVMPLGVF MPLLQLFPML RTDGNRGITA LPITIDGMFA DAFVHQFDRL 251 QRLLPKPLRL LQADLFFNFP HTAXVIADNL PATPSRRAET DTRGFQHNRF 301 MSLLRQGQCS AQTTQSAADD TGIGFQTALK FRINSMRINR TEIIRRQIFL 351 KIRANHCVCF IRCIFGRNDT GCRAISSXQK IG* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 237 shows 86.1% identity over a 382 aa overlap with a predicted ORF (ORF 237.ng) from N gonorrhoeae: m237/g237 10 20 30 40 50 60 m237. pep MRDKVGGΝVALPAPRIFDFDIGKLRKΝFKHILADKLGHTLRIVDKLVILTAEKQSAVRAE l l l l l l l h l l l l l l l l l M I M M M M I M M M M l l l l h l l l l l l l l M i l l g237 MRDKVGGΝIALPAPRIFDSΝIGKLRKΝFKHILADKLGHTRRIVDKFVILTAEKQPAVRAE 10 20 30 40 50 60 70 80 90 100 110 120 m237.pep AVIIQΝMAWAYCHIVTDKPFCARPQGFGRΝΝKGADSΝRLAFQRPEYRVQTCISIDSIDH 1 1 M ! I ! 1 1 1 M 1 1 1 1 : 1 1 1 1 1 1 1 M M I I I I I h 1 1 I I 1 1 I I I I I I 1 1 l l l h l l l g237 AVIIQΝMAWAYCHIVADKPFCARAQGFGGΝΝKGADGΝRLAFQRPEYRVQTDISIDGIDH 70 80 90 100 110 120 130 140 150 160 170 180 m237 . pep IFALDAAFGRVΝQPTVLMRFDARHRGLLEΝLRTGFGSGTSDAQSVSERMQVSGXGVELCP g237 IFTLDAAFGRVNQPTVLIRFDARHRGLLENLRAGFGSGAGNTQRVIERMKMPGQGIELCA 130 140 150 160 170 180 190 200 210 220 230 240 m237.pep LVHIAVKLLFQLGFIPKLIMTRTVMPLGVFMPLLQLFPMLRTDGNRGITALPITIDGMFA II III Mill III II II II Mill MMMMMMMMM III Mill II hill III g237 LVHIAVKLLFQLGFIPKLIMTRTVMPLGVFMPLLQLFPMLRTDGNRGITALPIAIDGMFA 190 200 210 220 230 240 250 260 270 280 290 300 m237. pep DAFVHQFDRLQRLLPKPLRLLQADLFFNFPHTAXVIADNLPATPSRRAETDTRGFQHNRF MMIIMMIMMMMMMMMMMM llhll 111 = = I g237 DAFVHQFDRLQRLLPKPLRLLQADLFFNFPHTAGWADNLPATPAGRAEADTCGFQNDGF 250 260 270 280 290 300 310 320 330 340 350 360 m237.pep MSLLRQGQCSAQTTQSAADDTGIGFQTALKFRINSMRINRTEIIRRQIFLKIRANHCVCF IhMI g237 MSVFRQRQCGTQTAQTAADNAGFGFQTALEFRINSMRINRTKIIRRQIFLKIRANHCVCF 310 320 330 340 350 360 370 380 m237.pep IRCIFGRNDTGCRAISSXQKIGX I Mlllll Mill g237 IGYIFGRNDTDCRAISSKQKIGX 370 380 The following partial DNA sequence was identified in N meningitidis <SEQ ID 885>: a237. seq 1 ATGCGGGACA AGGTTGGCGG TAATGTCGCA CTCCCCGCCC CACGAATATT 51 CGATTTTGAC ATCGGCAAGC TGCGGAAAAA CTTTAAGCAT ATCTTGGCGG 101 ACAAGCTCGG TCATACGCGC GGGATTGTCG ATAAACTCGT TATCCTTACC 151 GCCGAAAAGC AGTCTGCCGT CCGCGCTGAG GCGGTAATAA TCCAAAATAT 201 GACGGTTGTC GCATACTGCC ATATTGTTGC GGATAAGCCC TTTTGCACGC 251 GCGCCCAAGG GTTCTGTGGC AATAATAAAG GTGCTGACAG CAATCGCCTT 301 GCGCTCCAAA GGCTTGAATA TCGGATTCAA ACCGGCATAA GTATTGACGG 351 CGTACACCAG ATTTTTGCAT TCGACGCTGC CTTCGGGGGT GTAAACCAGC 401 CAACCGTTTT GATAAGGTTC AATGCGTATC ATGGGAGAAT GCTCAAAAAT 451 CTTCGTACCA GCTTCGGCAG CGGCGCGGGC GATGCCCAAC GTGTAATTGA 501 GCGGATGGAG ATGCCCGGAC AAGGGATCGA ACTGTGCGCC TTGGTACATA 551 TCGCTGTCAA GCTGCTGCTT CAGTTCAGTG TTATCCCAGA GTTGATAATG 601 AGTTGCACCG TAATATTTTT GGGCGTGCTC ATGCCATTGT TGCAATTCTT 651 CCCAATGCTG CGAACGGATG GCAACCGTGG CATAACCGCG CTGCCAATCG 701 CAATCAATGG CATGTTTGCG GACGCGTTCG TCCACCAGTT CGACCGCCTG 751 CAAAGACTGT TGCCAAAACC ATTGCGCTTG CTCCAAACCG ACCTGTTTTT 801 CAATTTCCTC CATACCGCAG GCGTAATCGC TGATAACCTG CCCGCCACTC 851 CGTCCCGACG CGCCGAAACC GATACGCGCG GCTTCCAACA CAACCGTTTC 901 ATGTCCCTGC TCCGCCAAGG GCAATGCAGT GCACAAACCA CTCAATCCGC 951 CGCCGATGAT ACAGGTATCG GTTTTCAGAC GGCATTGAAG TTTCGGATAA 1001 ACAGTATGAG GATTAACCGA ACTGAAATAA TAAGAAGGCA GATATTCTTG 1051 AAAATCAGGG CGAATCATTG TGTTTGCTTT ATCGGGTATA TTTTCGGACG 1101 GAATGATACA GGCTGTCGAG CCATATCGTC CAAACAGAAA ATCGGTTGA This corresponds to the amino acid sequence <SEQ ID 886; ORF 237. a>: a237.pep 1 MRDKVGGΝVA LPAPRIFDFD IGKLRKΝFKH ILADKLGHTR GIVDKLVILT 51 AEKQSAVRAE AVIIQΝMTW AYCHIVADKP FCTRAQGFCG ΝΝKGADΞΝRL 101 ALQRLEYRIQ TGISIDGVHQ IFAFDAAFGG VNQPTVLIRF NAYHGRMLKN 151 LRTSFGSGAG DAQRVIERME MPGQGIELCA LVHIAVKLLL QFSVIPELIM 201 SCTVIFLGVL MPLLQFFPML RTDGNRGITA LPIAINGMFA DAFVHQFDRL 251 QRLLPKPLRL LQTDLFFNFL HTAGVIADNL PATPSRRAET DTRGFQHNRF 301 MSLLRQGQCS AQTTQSAADD TGIGFQTALK FRINSMRINR TEIIRRQIFL 351 KIRANHCVCF IGYIFGRNDT GCRAISSKQK IG* m237/a237 85.6% identity in 382 aa overlap 10 20 30 40 50 60 m237. pep MRDKVGGNVALPAPRIFDFDIGKLRKNFKHILADKLGHTLRIVDKLVILTAEKQSAVRAE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a237 MRDKVGGNVALPAPRIFDFDIGKLRKNFKHILADKLGHTRGIVDKLVILTAEKQSAVRAE 10 20 30 40 50 60 70 80 90 100 110 120 m237.pep AVIIQNMAWAYCHIVTDKPFCARPQGFGRNNKGADSNRLAFQRPEYRVQTCISIDSIDH I I I I I I I: I I I I I I I I : I I I I I: I III I I I I I I I I I I I : I I I I I: I I Mil:: : a237 AVIIQNMTWAYCHIVADKPFCTRAQGFCGNNKGADSNRLALQRLEYRIQTGISIDGVHQ 70 80 90 100 110 120 130 140 150 160 170 180 m237. pep IFALDAAFGRVNQPTVLMRFDARHRGLLENLRTGFGSGTSDAQSVSERMQVSGXGVELCP 111:11111 1111111:11:1 I : I : I I I I : I I I I : : I I I I III:: I ill a237 IFAFDAAFGGVNQPTVLIRFNAYHGRMLKNLRTSFGSGAGDAQRVIERMEMPGQGIELCA 130 140 150 160 170 180 190 200 210 220 230 240 m237. pep LVHIAVKLLFQLGFIPKLIMTRTVMPLGVFMPLLQLFPMLRTDGNRGITALPITIDGMFA I I I I I I I I I : I :: I I : I I I : II: I I I : I I I I I : I I I I I I I I I I I I II I I I : I : I I I I a237 LVHIAVKLLLQFSVIPELIMSCTVIFLGVLMPLLQFFPMLRTDGNRGITALPIAINGMFA 190 200 210 220 230 240 250 260 270 280 290 300 m237. pep DAFVHQFDRLQRLLPKPLRLLQADLFFNFPHTAXVIADNLPATPSRRAETDTRGFQHNRF I I I I I I I I I I I I I I I I I I I I I I : I I I I I I III I I II I I I I I I I I I I I I I I I I I I I I I I a237 DAFVHQFDRLQRLLPKPLRLLQTDLFFNFLHTAGVIADNLPATPSRRAETDTRGFQHNRF 250 260 270 280 290 300 310 320 330 340 350 360 m237.pep MSLLRQGQCSAQTTQSAADDTGIGFQTALKFRINSMRINRTEIIRRQIFLKIRANHCVCF I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a237 MSLLRQGQCSAQTTQSAADDTGIGFQTALKFRINSMRINRTEIIRRQIFLKIRANHCVCF 310 320 330 340 350 360 370 380 m237.pep IRCIFGRNDTGCRAISSXQKIGX I I I I I I I I I I I I I I I I I I I I a237 IGYIFGRNDTGCRAISSKQKIGX 370 380 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 887>: g238.seq 1 atgaatttgc ctattcaaaa attcatgatg ctgttggcag cggcaatatc 51 gatgctgcat atccccatta gtcatgcgaa cggtttggat gcccgtttgc 101 gcgatgatat gcaggcaaaa cactacgaac cgggtggcaa ataccatctg 151 tttggtaatg ctcgcggcag tgttaaaaat cgggtttgcg ccgtccaaac 201 atttgatgca actgcggtcg gccccatact gcctattaca cacgaacgga 251 caggatttga aggtgttatc ggctatgaaa cccatttttc aggacacgga 301 cacgaagtac acagtccgtt cgataatcat gattcaaaaa gcacttctga 351 tttcagcggc ggcgtagacg gcggttttac cgtttaccaa cttcatcgga 401 cagggtcgga aatacatccc gcagacggat atgacgggcc tcaaggcggc 451 ggttatccgg aaccacaagg ggcaagggat atatacagct accatatcaa 501 aggaacttca accaaaacaa agataaacac tgttccgcaa gccccttttt 551 cagaccgctg gctaaaagaa aatgccggtg ccgcttccgg ttttctcagc 601 cgtgcggatg aagcaggaaa actgatatgg gaaaacgacc ccgataaaaa 651 ttggcgggct aaccgtatgg atgatattcg cggcatcgtc caaggtgcgg 701 ttaatccttt tttaacgggt tttcaagggg tagggattgg ggcaattaca 751 gacagtgcgg taagcccggt cacagataca gccgctcagc agactctaca 801 aggtattaat gatttaggaa atttaagtcc ggaagcacaa cttgccgccg 851 cgagcctatt acaggacagt gcctttgcgg taaaagacgg catcaattcc 901 gccagacaat gggctgatgc ccatccgaat ataacagcaa cagcccaaac 951 tgcccttgcc gtagcagagg ccgcaggtac ggtttggcgc ggtaaaaaag 1001 tagaacttaa cccgaccaaa tgggattggg ttaaaaatac cggctataaa 1051 aaacctgctg cccgccatat gcagactgta gatggggaga tggcaggggg 1101 gaatagaccg cctaaatcta taacgtcgga aggaaaagct aatgctgcaa 1151 cctatcctaa gttggttaat cagctaaatg agcaaaactt aaataacatt 1201 gcggctcaag atccaagatt gagtctagct attcatgagg gtaaaaaaaa 1251 ttttccaata ggaactgcaa cttatgaaga ggcagataga ctaggtaaaa 1301 tttgggttgg tgagggtgca agacaaacta gtggaggcgg atggttaagt 1351 agagatggca ctcgacaata tcggccacca acagaaaaaa aatcacaatt 1401 tgcaactaca ggtattcaag caaattttga aacttatact attgattcaa 1451 atgaaaaaag aaataaaatt aaaaatggac atttaaatat taggtaa This corresponds to the amino acid sequence <SEQ ID 888; ORF 238.ng>: g238.pep 1 MNLPIQKFMM LLAAAISMLH IPISHANGLD ARLRDDMQAK HYEPGGKYHL 51 FGNARGSVKN RVCAVQTFDA TAVGPI PIT HERTGFEGVI GYETHFSGHG 101 HEVHSPFDNH DSKSTSDFSG GVDGGFTVYQ LHRTGSEIHP ADGYDGPQGG 151 GYPEPQGARD IYSYHIKGTS TKTKINTVPQ APFSDRWLKE NAGAASGFLS 201 RADEAGKLIW ENDPDKNWRA NRMDDIRGIV QGAVNPFLTG FQGVGIGAIT 251 DSAVSPVTDT AAQQTLQGIN DLGNLSPEAQ LAAASLLQDS AFAVKDGINS 301 ARQWADAHPN ITATAQTALA VAEAAGTVWR GKKVELNPTK WDWVKNTGYK 351 KPAARHMQTV DGEMAGGNRP PKSITSEGKA NAATYPKLVN QLNEQNLNNI 401 AAQDPRLSLA IHEGKKNFPI GTATYEEADR LGKIWVGEGA RQTSGGGWLS 451 RDGTRQYRPP TEKKSQFATT GIQANFETYT IDSNEKRNKI KNGHLNIR* The following partial DNA sequence was identified in N meningitidis <SEQ ID 889>: m238. seq 1 ATGAATTTGC CTATTCAAAA ATTCATGATG CTGTTTGCAG CAGCAATATC 51 GTTGCTGCAA ATCCCCATTA GTCATGCGAA CGGTTTGGAT GCCCGTTTGC 101 GCGATGATAT GCAGGCAAAA CAcTACGAAC CGGGTGGTAA ATACCATCTG 151 TTTGGTAATG CTCGCGGCAG TGTTAAAAAG CGGGTTTACG CCGTCCAGAC 201 ATTTGATGCA ACTGCGGTCA GTCCTGTACT GCCTATTACA CACGAACGGA 251 CAGGGTTTGA AGGTGTTATC GGTTATGAAA CCCATTTTTC AGGGCACGGA 301 CATGAAGTAC ACAGTCCGTT CGATCATCAT GATTCAAAAA GCACTTCTGA 351 TTTCAGCGGC GGTGTAGACG GCGGTTTTAC TGTTTACCAA CTTCATCGAA 401 CAGGGTCGGA AATCCATCCG GAGGATGGAT ATGACGGGCC GCAAGGCAGC 451 GATTATCCGC CCCCCGGAGG AGCAAGGGAT ATATACAGCT ATTATGTCAA 501 AGGAACTTCA ACAAAAACAA AGACTAATAT TGTCCCTCAA GCCCCATTTT 551 CAGACCGTTG GCTAAAAGAA AATGCCGGTG CCGCCTCTGG TTTTTTCAGC 601 CGTGCGGATG AAGCAGGAAA ACTGATATGG GAAAGCGACC CCAATAAAAA 651 TTGGTGGGCT AACCGTATGG ATGATGTTCG CGGCATCGTC CAAGGTGCGG 701 TTAATCCTTT TTTAATGGGT TTTCAAGGAG TAGGGATTGG GGCAATTACA 751 GACAGTGCAG TAAGCCCGGT CACAGATACA GCCGCGCAGC AGACTCTACA 801 AGGTATTAAT GATTTAGGAA AATTAAGTCC GGAAGCACAA CTTGCTGCCG 851 CGAGCCTATT ACAGGACAGT GCTTTTGCGG TAAAAGACGG TATCAACTCT 901 GCCAAACAAT GGGCTGATGC CCATCCAAAT ATAACAGCTA CTGCCCAAAC 951 TGCCCTTTCC GCAGCAGAGG CCGCAGGTAC GGTTTGGAGA GGTAAAAAAG 1001 TAGAACTTAA CCCGACTAAA TGGGATTGGG TTAAAAATAC CGGTTATAAA 1051 AAACCTGCTG CCCGCCATAT GCAGACTTTA GATGGGGAGA TGGCAGGTGG 1101 GAATAAACCT ATTAAATCTT TACCAAACAG TGCCGCTGAA AAAAGAAAAC 1151 AAAATTTTGA GAAGTTTAAT AGTAACTGGA GTTCAGCAAG TTTTGATTCA 1201 GTGCACAAAA CACTAACTCC CAATGCACCT GGTATTTTAA GTCCTGATAA 1251 AGTTAAAACT CGATACACTA GTTTAGATGG AAAAATTACA ATTATAAAAG 1301 ATAACGAAAA CAACTATTTT AGAATCCATG ATAATTCACG AAAACAGTAT 1351 CTTGATTCAA ATGGTAATGC TGTGAAAACC GGTAATTTAC AAGGTAAGCA 1401 AGCAAAAGAT TATTTACAAC AACAAACTCA TATCAGGAAC TTAGACAAAT 1451 GA This corresponds to the amino acid sequence <SEQ LD 890; ORF 238>: m238.pep 1 MNLPIQKFMM LFAAAISLLQ IPISHANGLD ARLRDDMQAK HYEPGGKYHL 51 FGNARGSVKK RVYAVQTFDA TAVSPVLPIT HERTGFEGVI GYETHFSGHG 101 HEVHSPFDHH DSKSTSDFSG GVDGGFTVYQ LHRTGSEIHP EDGYDGPQGS 151 DYPPPGGARD IYSYYVKGTS TKTKTNIVPQ APFSDRWLKE NAGAASGFFS 201 RADEAGKLIW ESDPNKNWWA NRMDDVRGIV QGAVNPFLMG FQGVGIGAIT 251 DSAVSPVTDT AAQQTLQGIN DLGKLSPEAQ LAAASLLQDS AFAVKDGINS 301 AKQWADAHPN ITATAQTALS AAEAAGTVWR GKKVELNPTK WDWVKNTGYK 351 KPAARHMQTL DGEMAGGNKP IKSLPNSAAE KRKQNFEKFN SNWSSASFDS 401 VHKTLTPNAP GILSPDKVKT RYTSLDGKIT IIKDNENNYF RIHDNSRKQY 451 LDSNGNAVKT GNLQGKQAKD YLQQQTHIRN LDK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 238 shows 86.0% identity over a 401 aa overlap with a predicted ORF (ORF 238.ng) from N gonorrhoeae: m238/g238 10 20 30 40 50 60 m238. pep MΝLPIQKFMMLFAAAISLLQIPISHAΝGLDARLRDDMQAKHYEPGGKYHLFGΝARGSVKK g238 MΝLPIQKFMMLLAAAISMLHIPISHAΝGLDARLRDDMQAKHYEPGGKYHLFGΝARGSVKΝ 10 20 30 40 50 60 70 80 90 100 110 120 m238. pep RVYAVQTFDATAVSPVLPITHERTGFEGVIGYETHFSGHGHEVHSPFDHHDSKSTSDFSG II MMMMIhhMMMMMMMMMMMMMMMIhMMIMMM g238 RVCAVQTFDATAVGPILPITHERTGFEGVIGYETHFSGHGHEVHSPFDΝHDSKSTSDFSG 70 80 90 100 110 120 130 140 150 160 170 180 m238.pep GVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARDIYSYYVKGTSTKTKTΝIVPQ MMMMMMMMMM II I MMMIhMIMMM I III g238 GVDGGFTVYQLHRTGSEIHPADGYDGPQGGGYPEPQGARDIYSYHIKGTSTKTKIΝTVPQ 130 140 150 160 170 180 190 200 210 220 230 240 m238. pep APFSDRWLKEΝAGAASGFFSRADEAGKLIWESDPΝKΝWWAΝRMDDVRGIVQGAVΝPFLMG MMMMMIMMMhMMMMMIhlhMI I g238 APFSDRWLKEΝAGAASGFLSRADEAGKLIWEΝDPDKΝWRAΝRMDDIRGIVQGAVΝPFLTG 190 200 210 220 230 240 250 260 270 280 290 300 m238.pep FQGVGIGAITDSAVSPVTDTAAQQTLQGIΝDLGKLSPEAQLAAASLLQDSAFAVKDGIΝS g238 FQGVGIGAITDSAVSPVTDTAAQQTLQGIΝDLGΝLSPEAQLAAASLLQDSAFAVKDGIΝS 250 260 270 280 290 300 310 320 330 340 350 360 m238. pep AKQWADAHPΝITATAQTALSAAEAAGTVWRGKKVELΝPTKWDWVKΝTGYKKPAARHMQTL hMMMMMMMMhMMMMIMMMMMMMMMMMMMMIh g238 ARQWADAHPΝITATAQTALAVAEAAGTVWRGKKVELΝPTKWDWVKΝTGYKKPAARHMQTV 310 320 330 340 350 360 370 380 390 400 410 420 m238. pep DGEMAGGΝKPIKSLPΝSAAEKRKQΝFEKFΝSΝWSSASFDSVHKTLTPΝAPGILSPDKVKT IIMMIhl 11= =1 == - h =: = : = = = = g238 DGEMAGGΝRPPKSI -TSEGKAΝAATYPKLVΝQLΝEQΝLΝΝIAAQDPRLSLAIHEGKKΝFP 370 380 390 400 410 430 440 450 460 470 480 m238. pep RYTSLDGKITI IKDΝEΝΝYFRIHDΝSRKQYLDSΝGΝAVKTGΝLQGKQAKDYLQQQTHIRΝ g238 IGTATYEEADRLGKIWVGEGARQTSGGGWLSRDGTRQYRPPTEKKSQFATTGIQAΝFETY 420 430 440 450 460 470 The following partial DNA sequence was identified in N meningitidis <SEQ ID 891> • a238. seq (partial) 1 ATGAATTTGC CTATTCAAAA ATTCATGATG CTGTTTGCAG CAGCAATATC 51 GTTGCTGCAA ATCCCCATTA GTCATGCGAA CGGTTTGGAT GCCCGTTTGC 101 GCGATGATAT GCAGGCAAAA CACTACGAAC CGGGTGGTAA ATACCATCTG 151 TTTGGTAATG CTCGCGGCAG TGTTAAAAAT CGGGTTTACG CCGTCCAAAC 201 ATTTGATGCA ACTGCGGTCG GCCCCATACT GCCTATTACA CACGAACGGA 251 CAGGATTTGA AGGCATTATC GGTTATGAAA CCCATTTTTC AGGACATGGA 301 CATGAAGTAC ACAGTCCGTT CGATAATCAT GATTCAAAAA GCACTTCTGA 351 TTTCAGCGGC GGCGTAGACG GTGGTTTTAC CGTTTACCAA CTTCATCGGA 401 CAGGGTCGGA AATCCATCCG GAGGATGGAT ATGACGGGCC GCAAGGCAGC 451 GATTATCCGC CCCCCGGAGG AGCAAGGGAT ATATACAGCT ACTATGTCAA 501 AGGAACTTCA ACAAAAACAA AGAGTAATAT TGTTCCCCGA GCCCCATTTT 551 CAGACCGCTG GCTAAAAGAA AATGCCGGTG CCGCCTCTGG TTTTTTCAGC 601 CGTGCTGATG AAGCAGGAAA ACTGATATGG GAAAGCGACC CCAATAAAAA 651 TTGGTGGGCT AACCGTATGG ATGATATTCG CGGCATCGTC CAAGGTGCGG 701 TTAATCCTTT TTTAATGGGT TTTCAAGGAG TAGGGATTGG GGCAATTACA 751 GACAGTGCAG TAAGCCCGGT CACAGATACA GCCGCGCAGC AGACTCTACA 801 AGGTATTAAT CATTTAGGAA ATTTAAGTCC CGAAGCACAA CTTGCGGCTG 851 CAACCGCATT ACAAGACAGT GCTTTTGCGG TAAAAGACGG TATCAATTCC 901 GCCAGACAAT GGGCTGATGC CCATCCGAAT ATAACTGCAA CAGCCCAAAC 951 TGCCCTTGCC GTAGCAGAGG CCGCAACTAC GGTTTGGGGC GGTAAAAAAG 1001 TAGAACTTAA CCCGACCAAA TGGGATTGGG TTAAAAATAC CGGCTATAAA 1051 ACACCTGCTG TTCGCACCAT GCATACTTTG GATGGGGAAA TGGCCGGTGG 1101 GAATAGACCG CCTAAATCTA TAACGTCCAA CAGCAAAGCA GATGCTTCCA 1151 CACAA This corresponds to the amino acid sequence <SEQ ID 892; ORF 238.a>: a238.pep (partial) 1 MNLPIQKFMM LFAAAISLLQ IPISHANGLD ARLRDDMQAK HYEPGGKYHL 51 FGNARGSVKN RVYAVQTFDA TAVGPILPIT HERTGFEGII GYETHFSGHG 101 HEVHSPFDNH DSKSTSDFSG GVDGGFTVYQ LHRTGSEIHP EDGYDGPQGS 151 DYPPPGGARD IYSYYVKGTS TKTKSNIVPR APFSDRWLKE NAGAASGFFS 201 RADEAGKLIW ESDPNKNWWA NRMDDIRGIV QGAVNPFLMG FQGVGIGAIT 251 DSAVSPVTDT AAQQTLQGIN HLGNLSPEAQ LAAATALQDS AFAVKDGINS 301 ARQWADAHPN ITATAQTALA VAEAATTVWG GKKVELNPTK WDWVKNTGYK 351 TPAVRTMHTL DGEMAGGNRP PKSITSNSKA DASTQ m238/a238 91.9% identity in 385 aa overlap 10 20 30 40 50 60 m238. pep MNLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEPGGKYHLFGNARGSVKK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : a238 MNLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEPGGKYHLFGNARGSVKN 10 20 30 40 50 60 70 80 90 100 110 120 m238.pep RVYAVQTFDATAVSPVLPITHERTGFEGVIGYETHFSGHGHEVHSPFDHHDSKSTSDFSG I I I I I I I I I I I I I : I : I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I a238 RVYAVQTFDATAVGPILPITHERTGFEGIIGYETHFSGHGHEVHSPFDNHDSKSTSDFSG 70 80 90 100 110 120 130 140 150 160 170 180 m238.pep GVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARDIYSYYVKGTSTKTKTNIVPQ I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I : a238 GVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARDIYSYYVKGTSTKTKSNIVPR 130 140 150 160 170 180 190 200 210 220 230 240 m238. pep APFSDRWLKENAGAASGFFSRADEAGKLIWESDPNKNWWANRMDDVRGIVQGAVNPFLMG I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I II I I : I I I I I I I I I I I I I I a238 APFSDRWLKENAGAASGFFSRADEAGKLIWESDPNKNWWANRMDDIRGIVQGAVNPFLMG 190 200 210 220 230 240 250 260 270 280 290 300 m238.pep F Q GVGIGAITDSAVSPVTDTAAQQTLQGINDLGKLSPEAQLAAASLLQDSAFAVKDGINS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I : I I I I I I I II I I I I I a238 FQGVGIGAITDSAVSPVTDTAAQQTLQGINHLGNLSPEAQLAAATALQDSAFAVKDGINS 250 260 270 280 290 300 310 320 330 340 350 360 m238. pep AKQWADAHPNITATAQTALSAAEAAGTVWRGKKVELNPTKWDWVKNTGYKKPAARHMQTL I : I I I I I I I I I I I I I I I I I:: I I I I III I I I I I I I I I I I I I I I I I I I I I I: I I: I I a238 ARQWADAHPNITATAQTALAVAEAATTVWGGKKVELNPTKWDWVKNTGYKTPAVRTMHTL 310 320 330 340 350 360 370 380 390 400 410 419 m238.pep DGEMAGGNKPIKSLP-NSAAEKRKQNFEKFNSNWSSASFDSVHKTLTPNAPGILSPDKVK I I I I I I I I : I I I : II I: I a238 DGEMAGGNRPPKSITSNSKADASTQ The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 893>: g239.seq 1 atgttccacc ataaaggtat tgcccgaaac cggcggatgg aggttttgtt 51 tttctgccgc cgccctgatc gcttcgtgat tcgccaaacg cgcctgttgc 101 agcctcattt gcgcataatc ctgctccaag gcgatttcct gttttttcgc 151 cttgtccaaa gctgtgaagt tgagcctgta ctggttttgc tgcatcacaa 201 cggaaaaagc ggaaacgcac accgcaagca gcagaaagaa attcgatttg 251 ttcattgccg ttcagacgtt tttctctgtt attattccgg tatcggaccg 301 gcagtccgct ccgccacacg caaaactgcg ctcctcgccc tcgggttggc 351 ggcaatttcc gcttcacccg gctttaatgc cctgcccacg attttcaggg 401 gcggatcggg caaatccgct tctctgaccg ccgcccagct cggcaggggc 451 tcgtgttgcg aatatttttt gacaaactgc ttcacaatgc ggtcttccaa 501 cgaatggaaa gcaatgaccg ccaaacgccc gccctctttc agacggcaca 551 tgacctgcgg caataccgcc cctacttctt caagctcgcg gttaataaag 601 atgcggattg cctggaaggt gcgcgtcgca ggatcctgcc cccgctcgcg 651 agtacggacg ttttgtgcca cgatctgcgc cagcttgcgg gttgtatcga 701 ttggactttc cgcccgttgc gcgacaatgg cgcgcacaat ctggcggcta 751 aaccgctctt caccataa This corresponds to the amino acid sequence <SEQ ID 894; ORF 239.ng>: g239.pep 1 MFHHKGIARΝ RRMEVLFFCR RPDRFVIRQT RLLQPHLRII LLQGDFLFFR 51 LVQSCEVEPV LVLLHHΝGKS GΝAHRKQQKE IRFVHCRSDV FLCYYSGIGP 101 AVRSATRKTA LLALGLAAIS ASPGFΝALPT IFRGGSGKSA SLTAAQLGRG 151 SCCEYFLTΝC FTMRSSΝEWK AMTAKRPPSF RRHMTCGΝTA PTSSSSRLIK 201 MRIAWKVRVA GSCPRSRVRT FCATICASLR WSIGLSARC ATMARTIWRL 251 ΝRSSP* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 895>: m239.seq 1 ATGCTCCACC ATAAAGGTmy kGCCCGAAAC CGGCkGATGG AGGTTTTGTT 51 TTTCTGCCGC CGCCCTGATC GCTTCGTGGT TCGCCAAACG CGCCTGTTGC 101 AGCCTCATTT GCGCATAATC CTGCTCCAAG GCGATTTCCT GTTTTTTCGC 151 CTTATCCAAA GCTGTGAAAT TGAGCCTGTA CTGGTTTTGC TGCATCACAA 201 CGGAAAAAGC GGAAACGCAC ACCGCAAGCA GCAGAAGGAA ATTCAATTTG 251 TTCATTGCCA TTCAGACGTT TTTCTCTGTG ATTGTTCCGG TATCGGACCG 301 GCAGTCCGCT CCGCCACACG CAAAACCGCA CTTCTCGCCC TCGGATTGGC 351 GGCAATTTCC GCCTCACCCG GCTTTAATGC CCTGCCCACG ATTTTCAGGG 401 GCAGCTCGGG CAAATCCGCT TCCCTGaCCG CCGCCCAGCG CGGCAGGGGC 451 GCGTGTTGCG AATATTTTTT GACAAACTGC TTCACAATGC GATCTTCCAA 501 CGAATGGAAA GCAATGACCG CCAAACGTCC GCCCTCTTTC AGACGACACA 551 TGACCTGCGG CAATACTGCC CCTACTTCTT CAAGCTCGCG GTTAATAAAG 601 ATGCGGACCG CCTGGAAGGT GCGCGTCGCA GGATCCTGCC CCCGCTCGCG 651 AGTACGGACG TTTTGTGCCA CGATCTGCGC CAGCTTGCGG GTTGTATCGA 701 TTGGACTTTC CGCCCGTTGC GCAACAATGG CGCGCGCAAT cCGGCGGCTa 751 AACCGCTCTT CACCATAA This corresponds to the amino acid sequence <SEQ ID 896; ORF 239>: m239.pep 1 MLHHKGXARN RXMEVLFFCR RPDRFWRQT RLLQPHLRII LLQGDFLFFR 51 LIQSCEIEPV LVLLHHNGKS GNAHRKQQKE IQFVHCHSDV FLCDCSGIGP 101 AVRSATRKTA LLALGLAAIS ASPGFNALPT IFRGSSGKSA SLTAAQRGRG 151 ACCEYFLTNC FTMRSSNEWK AMTAKRPPSF RRHMTCGNTA PTSSSSRLIK 201 MRTAWKVRVA GSCPRSRVRT FCATICASLR WSIGLSARC ATMARAIRRL 251 NRSSP* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 239 shows 93.7% identity over a 255 aa overlap with a predicted ORF (ORF 239.ng) from N gonorrhoeae: m239/g239 10 20 30 40 50 60 m239.pep MLHHKGXARΝRXMEVLFFCRRPDRFWRQTRLLQPHLRIILLQGDFLFFRLIQSCEIEPV hllll MM MMMMMMIhMMMMMMMMMMMIhlllhMI g239 MFHHKGIARΝRRMEVLFFCRRPDRFVIRQTRLLQPHLRIILLQGDFLFFRLVQSCEVEPV 10 20 30 40 50 60 70 80 90 100 110 120 m239. pep LVLLHHΝGKSGΝAHRKQQKEIQFVHCHSDVFLCDCSGIGPAVRSATRKTALLALGLAAIS I II I II I II II II II 11 II I II II I g239 LVLLHHΝGKSGΝAHRKQQKEIRFVHCRSDVFLCYYSGIGPAVRSATRKTALLALGLAAIS 70 80 90 100 110 120 130 140 150 160 170 180 m239. pep ASPGFΝALPTIFRGSSGKSASLTAAQRGRGACCEYFLTΝCFTMRSSΝEWKAMTAKRPPSF 111 M 1111111 M II 11111111 MhlMMMMMMMMIMMMIMM g239 ASPGFΝALPTIFRGGSGKSASLTAAQLGRGSCCEYFLTΝCFTMRSSΝEWKAMTAKRPPSF 130 140 150 160 170 180 190 200 210 220 230 240 m239.pep RRHMTCGΝTAPTSSSSRLIKMRTAWKVRVAGSCPRSRVRTFCATICASLRWSIGLSARC MMMMMMMMMMM II 111 II I II II 1111 II I II 11 II M 111 II 111 II g239 RRHMTCGΝTAPTSSSSRLIKMRIAWKVRVAGSCPRSRVRTFCATICASLRWSIGLSARC 190 200 210 220 230 240 250 m239.pep ATMARAIRRLΝRSSPX M 11l1l1l1-•11 llll g239 ATMARTIWRLΝRSSPX 250 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 897>: a239. seq 1 ATGCTCCACC ATAAAGGTAT TGCCCGAAAC CGGCGGATGG AGGTTTTGTT 51 TTTCTGCCGC CGCCCTGATC GCTTCGTGGT TCGCCAAACG CGCCTGTTGC 101 AGCCTCATTT GCGCATAATC CTGCTCCAAG GCGATTTCCT GTTTTTTCGC 151 CTTATCCAAA GCTGTGAAGT TGAGCCTGTA CTGGTTTTGC TGCATCACAA 201 CGGAAAAAGC GGAAACGCAC ACCGCAAGCA GCAGAAGGAA ATTCAATTTG 251 TTCATTGCCA TTCAGACGTT TTTCTCTGTG ATTGTTCCGG TATCGGACCG 301 GCAGTCCGCT CCGCCACACG CAAAACCGCA CTTCTCGCCC TCGGATTGGC 351 GGCAATTTCC GCCTCACCCG GCTTTAATGC CCTGCCCGCG ATTTTCAGGG 401 GCGGCTCGGG CAAATCCGCT TCCCTGACCG CCGCCCAGCG CGGCAGGGGC 451 GCGTGTTGCG AATATTTTTT GACAAACTGC TTCACAATGC GGTCTTCCAA 501 CGAATGGAAA GCAATGACCG CCAAACGTCC GCCCTCTTTC AGACGACACA 551 TGACCTGCGG CAATACTGCC CCTACTTCTT CAAGCTCGCG GTTAATAAAG 601 ATGCGGATTG CCTGGAAGGT GCGCGTCGCA GGATCCTGCC CCCGCTCGCG 651 AGTACGGACG TTTTGTGCCA CGATCTGCGC CAGCTTGCGG GTTGTATCGA 701 TTGGACTTTC CGCCCGTTGC GCAACAATGG CGCGCGCAAT CTGGCGGCTA 751 AACCGCTCTT CACCATAA This corresponds to the amino acid sequence <SEQ ID 898; ORF 239.a>: a239.pep 1 MLHHKGIARN RRMEVLFFCR RPDRFWRQT RLLQPHLRII LLQGDFLFFR 51 LIQSCEVEPV LVLLHHNGKS GNAHRKQQKE IQFVHCHSDV FLCDCSGIGP 101 AVRSATRKTA LLALGLAAIS ASPGFNALPA IFRGGSGKSA SLTAAQRGRG 151 ACCEYFLTNC FTMRSSNEWK AMTAKRPPSF RRHMTCGNTA PTSSSSRLIK 201 MRIAWKVRVA GSCPRSRVRT FCATICASLR WSIGLSARC ATMARAIWRL 251 NRSSP* m239/a239 97.3% identity in 255 aa overlap 10 20 30 40 50 60 m239.pep MLHHKGXARNRXMEVLFFCRRPDRFWRQTRLLQPHLRIILLQGDFLFFRLIQSCEIEPV llllll I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I a239 MLHHKGIARNRRMEVLFFCRRPDRFWRQTRLLQPHLRIILLQGDFLFFRLIQSCEVEPV 10 20 30 40 50 60 70 80 90 100 110 120 m239. pep LVLLHHNGKSGNAHRKQQKEIQFVHCHSDVFLCDCSGIGPAVRSATRKTALLALGLAAIS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a239 LVLLHHNGKSGNAHRKQQKEIQFVHCHSDVFLCDCSGIGPAVRSATRKTALLALGLAAIS 70 80 90 100 110 120 130 140 150 160 170 180 m239.pep ASPGFNALPTIFRGSSGKSASLTAAQRGRGACCEYFLTNCFTMRSSNEWKAMTAKRPPSF I I I I I I I I I : I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a239 ASPGFNALPAIFRGGSGKSASLTAAQRGRGACCEYFLTNCFTMRSSNEWKAMTAKRPPSF 130 140 150 160 170 180 190 200 210 220 230 240 m239. pep RRHMTCGNTAPTSSSSRLIKMRTAWKVRVAGSCPRSRVRTFCATICASLRWSIGLSARC I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a239 RRHMTCGNTAPTSSSSRLIKMRIAWKVRVAGSCPRSRVRTFCATICASLRWSIGLSARC 190 200 210 220 230 240 250 m239.pep ATMARAIRRLNRSΞPX a239 ATMARAIWRLNRSSPX 250 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 899>: g240.seq 1 atgatagaag tcatacattt cttcggcgcc gaaacgcgca gacagtttgc 51 ttgtgccgac gttggacgat ttctgcataa tgccgcgcac atccaaagag 101 gggtaaacat gggtatcatc gcgcacggga gacggtccga ttttataagg 151 ctgcgtattc agccgttcgt tcaaatcggt tttgcccgca tccaatgcct 201 tcgcaatcac gaacggtttg attgccgaac caggttcgat catatcggtt 251 acggcacggt tgcgccgctg ttcgctgtct gcccggccgg gtctgttggg 301 atcgtaggcg ggcgtattgg ccaaggcgag gatttccccc gtgcgggcat 351 ccaaaaccac caccgttccg gcttttgcct gatggtattc gaccgccttg 401 ttcaactctt cataggccaa ggtctgaatc ctctgatcga gggaaaggat 451 gatgtctttg ccgttttgcg gtgctttatt gcgcggggag tccaagctgt 501 ccacaatatt gccctgccgg tcccgcaaaa caacttccgc gccgtcttcg 551 ccatacaggc tgtcttcaag cgaaagttcc aaaccttcct gacctttgcc 601 gtcaatatcg gtaaatccga tgacgtgtgc aaacaggttg cccatcgggt 651 aatggcgttt taa This corresponds to the amino acid sequence <SEQ ID 900; ORF 240.ng>: g240.pep 1 MIEVIHFFGA ETRRQFACAD VGRFLHΝAAH IQRGVΝMGII AHGRRSDFIR 51 LRIQPFVQIG FARIQCLRΝH ERFDCRTRFD HIGYGTVAPL FAVCPAGSVG 101 IVGGRIGQGE DFPRAGIQΝH HRSGFCLMVF DRLVQLFIGQ GLΝPLIEGKD 151 DVFAVLRCFI ARGVQAVHΝI ALPVPQΝΝFR AVFAIQAVFK RKFQTFLTFA 201 VNIGKSDDVC KQVAHRVMAF* The following partial DNA sequence was identified in N meningitidis <SEQ ID 901>: m240. seq 1 ATGATAGAAG TCATACATTT CTTCGGCACC GAAACGCGCA GACAGTTTGC 51 TTGTGCCGAC GTTGGACGAT TTCTGCATGA TGCCGCGCAC ATCCAAAGAG 101 GGGTAAACAT GGGTATCGCG CACGGGAGAC GGTCCGATTT TATAAGGCTG 151 CGTATTGAGC CGTTCGTTCA AATCGGTTTT GCCCGCATCC AATGCCTTCG 201 CAATCACAAA CGGTTTGATT GCCGAACCGG GTTCGATCAT ATCGGTTACG 251 GCACGGTTGC GCCGCTGTTC GCTGTCTGCC CGGCCGGGCC TGTTGGGATC 301 GTAGGCGGGC GTATTGGCCA AGGCGAGGAT TTCCCCCGTG CGGGCATCCA 351 ArACCACCAC CGTTCCGGCT TTTGCCTGAT GGTATTCGAC CGCCTTGTTC 401 AACTCTTCAT AGGCCAAGGT CTGAATCCTC TGATCGAGGG AAAGGATGAT 451 GTCTTTGCCG TTTTTCGGGG CTTTAkTGCG CGGGGAGTCC AAGCTGTCCA 501 CAATATTGCC CTGCCGGTCC CGCAAAACGA CTTCCGCGCC GTCTTCGCCA 551 TGCAAGCTGT CTTCAAGCGA AAGTTCCAAA CCTTCCTGAC CTTTGCCGTC 601 AATATCGGTA AATCCGATGA CGTGTGCAAA CAGGTTGCCC ATCGGGTAAT 651 GGCGTTTTAA This corresponds to the amino acid sequence <SEQ ID 902; ORF 240>: m240. pep 1 MIEVIHFFGT ETRRQFACAD VGRFLHDAAH IQRGVΝMGIA HGRRSDFIRL 51 RIQPFVQIGF ARIQCLRΝHK RFDCRTGFDH IGYGTVAPLF AVCPAGPVGI 101 VGGRIGQGED FPRAGIQXHH RSGFCLMVFD RLVQLFIGQG LΝPLIEGKDD 151 VFAVFRGFXA RGVQAVHΝIA LPVPQΝDFRA VFAMQAVFKR KFQTFLTFAV 201 ΝIGKSDDVCK QVAHRVMAF* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 240 shows 94.5% identity over a 220 aa overlap with a predicted ORF (ORF 240.ng) from N gonorrhoeae: m240/g240 10 20 30 40 50 59 m240.pep MIEVIHFFGTETRRQFACADVGRFLHDAAHIQRGVΝMGI-AHGRRSDFIRLRIQPFVQIG M M M M M I M I M M M g240 MIEVIHFFGAETRRQFACADVGRFLHΝAAHIQRGVΝMGIIAHGRRSDFIRLRIQPFVQIG 10 20 30 40 50 60 60 70 80 90 100 110 119 m240. pep FARIQCLRΝHKRFDCRTGFDHIGYGTVAPLFAVCPAGPVGIVGGRIGQGEDFPRAGIQXH M M M M M M I M M M I I I I I I I I I I I I I I I I 1 1 1 1 I g240 FARIQCLRΝHERFDCRTRFDHIGYGTVAPLFAVCPAGSVGIVGGRIGQGEDFPRAGIQΝH 70 80 90 100 110 120 120 130 140 150 160 170 179 m240.pep HRSGFCLMVFDRLVQLFIGQGLΝPLIEGKDDVFAVFRGFXARGVQAVHΝIALPVPQΝDFR 111111111 i 1111 j 1111111111111111111 h I I g240 HRSGFCLMVFDRLVQLFIGQGLΝPLIEGKDDVFAVLRCFIARGVQAVHΝIALPVPQΝΝFR 130 140 150 160 170 180 180 190 200 210 220 m240. pep AVFAMQAVFKRKFQTFLTFAVΝIGKSDDVCKQVAHRVMAFX MlhlMMMMMMMMMMMMMMMMM g240 AVFAIQAVFKRKFQTFLTFAVΝIGKSDDVCKQVAHRVMAF 190 200 210 220 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 903>: a240.seq 1 ATGATAGAAG TCATACATTT CTTCGGCACC GAAACGCGCA GACAGTTTGC 51 TTGTGCCGAC GTTGGACGAT TTCTGCATGA TGCCGCGCAC ATCCAAAGAG 101 GGGTAAACAT GGGTATCGCG CACGGGAGAC GGTCCGATTT TATAAGGCTG 151 CGTATTCAGC CGTTCGTTCA AATCGGTTTT GCCCGCATCC AATGCCTTCG 201 CAATCACAAA CGGTTTGATT GCCGAACCGG GTTCGATCAT ATCGGTTACG 251 GCACGGTTGC GCCGCTGTTC GCTGTCTGCC CGGCCGGGCC TGTTGGGATC 301 GTAGGCGGGC GTATTGGCCA AGGCGAGGAT TTCCCCCGTG CGGGCATCCA 351 AAACCACCAC CGTTCCGGCT TTTGCCTGAT GGTATTCGAC CGCCTTGTTC 401 AACTCTTCAT AGGCCAAGGT CTGAATCCTC TGATCGAGGG AAAGGATGAT 451 GTCTTTGCCG TTTTTCGGGG CTTTATTGCG CGGGGAGTCC AAGCTGTCCA 501 CAATATTGCC CTGCCGGTCC CGCAAAACGA CTTCCGCGCC GTCTTCGCCA 551 TGCAGGCTGT CTTCAAGCGA AAGTTCCAAA CCTTCCTGAC CTTTGCCGTC 601 AATATCGGTA AATCCGATGA CGTGTGCAAA CAGGTTGCCC ATCGGGTAAT 651 GGCGTTTTAA This corresponds to the amino acid sequence <SEQ ID 904; ORF 240. a>: a240.pep 1 MIEVIHFFGT ETRRQFACAD VGRFLHDAAH IQRGVNMGIA HGRRSDFIRL 51 RIQPFVQIGF ARIQCLRNHK RFDCRTGFDH IGYGTVAPLF AVCPAGPVGI 101 VGGRIGQGED FPRAGIQNHH RSGFCLMVFD RLVQLFIGQG LNPLIEGKDD 151 VFAVFRGFIA RGVQAVHNIA LPVPQNDFRA VFAMQAVFKR KFQTFLTFAV 201 NIGKSDDVCK QVAHRVMAF* m240/a240 99.1 % identity in 219 aa overlap 10 20 30 40 50 60 m2 0.pep MIEVIHFFGTETRRQFACADVGRFLHDAAHIQRGVNMGIAHGRRSDFIRLRIQPFVQIGF I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a240 MIEVIHFFGTETRRQFACADVGRFLHDAAHIQRGVNMGIAHGRRSDFIRLRIQPFVQIGF 10 20 30 40 50 60 70 80 90 100 110 120 m240.pep ARIQCLRNHKRFDCRTGFDHIGYGTVAPLFAVCPAGPVGIVGGRIGQGEDFPRAGIQXHH I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II a240 ARIQCLRNHKRFDCRTGFDHIGYGTVAPLFAVCPAGPVGIVGGRIGQGEDFPRAGIQNHH 70 80 90 100 110 120 130 140 150 160 170 180 m240. pep RSGFCLMVFDRLVQLFIGQGLNPLIEGKDDVFAVFRGFXARGVQAVHNIALPVPQNDFRA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I a240 RSGFCLMVFDRLVQLFIGQGLNPLIEGKDDVFAVFRGFIARGVQAVHNIALPVPQNDFRA 130 140 150 160 170 180 190 200 210 220 m240. pep VFAMQAVFKRKFQTFLTFAVNIGKSDDVCKQVAHRVMAFX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a240 VFAMQAVFKRKFQTFLTFAVNIGKSDDVCKQVAHRVMAFX 190 200 210 220 The following partial DNA sequence was identified in N gonorrhoeae <SEQ TD 905>: g241.seq 1 ATGATAGAAG TCATACATTT CTTCGGCACC GAAACGCGCA GACAGTTTGC 51 TTGTGCCGAC GTTGGACGAT TTCTGCATGA TGCCGCGCAC ATCCAAAGAG 101 GGGTAAACAT GGGTATCGCG CACGGGAGAC GGTCCGATTT TATAAGGCTG 151 CGTATTCAGC CGTTCGTTCA AATCGGTTTT GCCCGCATCC AATGCCTTCG 201 CAATCACAAA CGGTTTGATT GCCGAACCGG GTTCGATCAT ATCGGTTACG 251 GCACGGTTGC GCCGCTGTTC GCTGTCTGCC CGGCCGGGCC TGTTGGGATC 301 GTAGGCGGGC GTATTGGCCA AGGCGAGGAT TTCCCCCGTG CGGGCATCCA 351 ArACCACCAC CGTTCCGGCT TTTGCCTGAT GGTATTCGAC CGCCTTGTTC 401 AACTCTTCAT AGGCCAAGGT CTGAATCCTC TGATCGAGGG AAAGGATGAT 451 GTCTTTGCCG TTTTTCGGGG CTTTAkTGCG CGGGGAGTCC AAGCTGTCCA 501 CAATATTGCC CTGCCGGTCC CGCAAAACGA CTTCCGCGCC GTCTTCGCCA 551 TGCAAGCTGT CTTCAAGCGA AAGTTCCAAA CCTTCCTGAC CTTTGCCGTC 601 AATATCGGTA AATCCGATGA CGTGTGCAAA CAGGTTGCCC ATCGGGTAAT 651 GGCGTTTTAA This corresponds to the amino acid sequence <SEQ ID 906; ORF 241. ng>: g241.pep 1 MPTRPTRAAΝ PPTPTTWLQT AYCPRPPYRP PSVQTHTPHE PASSTCAAKS 51 ANRRENSHNA QPTYLLHPSN KMPSETEQTL FRRHQIPPSC RQSVWMTVR 101 TVDMTVCDFL IGCIAHAFNR SFKADFHACQ RMVAVHHRLA VGNIGYTIDD 151 NIAGFRIVRF KHHTDLDFNR ERARIFNTDQ LRIMLTERIV GRKRHFDRIA 201 GILTVQRLFH QRENA TAV QIRNRFFGFI QKLIVGIIHL IMQRNHGIFC 251 NSHICPFRNS RLITGAF* The following partial DNA sequence was identified in N meningitidis <SEQ ID 907>: m241.seq (partial) 1 .. CGGCAATCAG TGGTGGTGAT GACCGTGCGG GCCGTGGACA TGACCGTGTG 51 CGATTTCCTC ATCGGATGCA TCGCGCACGC TTTCAACTGT AGCCTTAAAG 101 CGGATTTTCA TGCCTGCCAA AGGATGGTTG CCGTCCACCA CCGCCTTGCC 151 GTCGGCAACA TCGGTTACAC GATAGACGAC AACATCGCCG GTTTCAGGAT 201 CGTCGGCTTC AAACATCATG CCGACTTCGA CTTCAACAGG GAACACGCCC 251 GCATCTTCGA TACGGACCAA CTCCGGATCC TGCTCGCCGA ACGCATCGTC 301 GGGCGACAGC GCCACATCGA CCGTATCGCC GGCATCCTTA CCGTGCAACG 351 CCTCTTCCAC CAAAGGGAAA ATGCCGTCGT AACCGCCGTG CAGATACGCA 401 ATCGGTTCTT CGGTTTTGTC CAAAAGCTGA TTGTTGGCAT CATACATCTC 451 ATAATGCAGC GAAACCACGG AATTTTTCAC GATAGCCATA TTTGTCCTTT 501 CAGGAACAGC AGATTAATTA CAGGCGCATT CTAA This corresponds to the amino acid sequence <SEQ ID 908; ORF 241>: m241.pep (partial) 1 ..RQSVWMTVR AVDMTVCDFL IGCIAHAFΝC SLKADFHACQ RMVAVHHRLA 51 VGNIGYTIDD NIAGFRIVGF KHHADFDFNR EHARIFDTDQ LRILLAERIV 101 GRQRHIDRIA GILTVQRLFH QRENAWTAV QIRNRFFGFV QKLIVGIIHL 151 IMQRNHGIFH DSHICPFRNS RLITGAF* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 241 shows 91.5% identity over a 177 aa overlap with a predicted ORF (ORF 241.ng) from N gonorrhoeae: m241/g241 10 20 30 m241 . pep RQSVWMTVRAVDMTVCDFLIGCIAHAFΝC g241 QPTYLLHPSΝKMPSETEQTLFRRHQIPPSCRQSWVMTVRTVDMTVCDFLIGCIAHAFΝR 70 80 90 100 110 120 40 50 60 70 80 90 m241 . pep SLKADFHACQRMVAVHHRLAVGΝIGYTIDDΝIAGFRIVGFKHHADFDFΝREHARIFDTDQ h M M M M M M M M M I M M M M I M M M M l l l h h l l l l h l l l h l l l g241 SFKADFHACQRMVAVHHRLAVGΝIGYTIDDΝIAGFRIVRFKHHTDLDFΝRERARIFΝTDQ 130 140 150 160 170 180 100 110 120 130 140 150 m241.pep LRILLAERIVGRQRHIDRIAGILTVQRLFHQREΝAWTAVQIRΝRFFGFVQKLIVGIIHL MhhMMIhlhMMMMMMMMMMMMMMMMhlMIMMM g241 LRIMLTERIVGRKRHFDRIAGILTVQRLFHQREΝAWTAVQIRΝRFFGFIQKLIVGIIHL 190 200 210 220 230 240 160 170 m241.pep IMQRΝHGIFHDSHICPFRΝSRLITGAFX M M M M M M M M M g241 IMQRΝHGIFCΝSHICPFRΝSRLITGAFX 250 260 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 909>: a241.seq 1 ATGCCAACAC GTCCAACTCG CGCCGCAAAG CATCCAACCC CGCCAACCTG 51 GCTTCAGACG GCATACTGCC CTCGTCCGCC ATATCGTCCG CCGTCCGTGC 101 AAACGCATAC ACCGCATGAA CCGGCTTCCT CAACCTGCGC GGCAAAATCA 151 GCGAACCGAC GGGAAAATTT TCATAATGCC CAACCGACAT ACCTTCTCCA 201 TCCATCAAAC AAAATGCCGT CTGAAATGGA ACAAACCCTT TTCAGACGGC 251 ATCAGATACC TCCAAGCTGC CGGCAATCAG TGGTGGTGAT GACCGTGCGG 301 ACCGTGGACA TGACCGTGTG CGATTTCCTC ATCGGATGCA TCGCGCACAC 351 TTTCAACCGT AGCCTTAAAG CGGATTTTCA TGCCTGCCAA AGGATGGTTG 401 CCGTCCACCA CCGCCTTACC GTCGGCAACA TCGGTTACAC GATAGACGAC 451 AACATCGCCG GTTTCAGGAT CGTCGGCTTC AAACATCATG CCGACTTCGA 501 CTTCAACAGG GAACACGCCC GCATCTTCAA TACGGACCAA CTCCGGATCC 551 TGCTCGCCGA ACGCATCGTC GGGCGAAAGC GCCACATCGA CCGTATCGCC 601 GGCATCCTTA CCGTGCAACG CCTCTTCCAC CAAAGGGAAA ATGCCGTCGT 651 AACCGCCGTG CAGATACGCA ATCGGTTCTT CGGTTTTGTC CAAAAGCTGA 701 TTGTTGGCAT CATACATCTC ATAATGCAGC GAAACCACGG AATTCTTCAC 751 GATAGCCATA TTTGTCCTTT CAGGAACAGC AGATTAATTA CAGGCGCATT 801 CTAA This corresponds to the amino acid sequence <SEQ ID 910; ORF 241.a>: a241.pep 1 MPTRPTRAAK HPTPPTWLQT AYCPRPPYRP PSVQTHTPHE PASSTCAAKS 51 ANRRENFHNA QPTYLLHPSN KMPSEMEQTL FRRHQIPPSC RQSVWMTVR 101 TVDMTVCDFL IGCIAHTFNR SLKADFHACQ RMVAVHHRLT VGNIGYTIDD 151 NIAGFRIVGF KHHADFDFNR EHARIFNTDQ LRILLAERIV GRKRHIDRIA 201 GILTVQRLFH QRENAWTAV QIRNRFFGFV QKLIVGIIHL IMQRNHGILH 251 DSHICPFRNS RLITGAF* m24 l/a241 96.0% identity in 177 aa overlap 10 20 30 m241 . pep RQSVWMTVRAVDMTVCDFLIGCIAHAFNC I I I I I I I I I I : I I I I I I I I I I I I I I I : I I a241 QPTYLLHPSNKMPSEMEQTLFRRHQIPPΞCRQSVWMTVRTVDMTVCDFLIGCIAHTFNR 70 80 90 100 110 120 40 50 60 70 80 90 m2 1.pep ΞLKADFHACQRMVAVHHRLAVGNIGYTIDDNIAGFRIVGFKHHADFDFNREHARIFDTDQ I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I a241 SLKADFHACQRMVAVHHRLTVGNIGYTIDDNIAGFRIVGFKHHADFDFNREHARIFNTDQ 130 140 150 160 170 180 100 110 120 130 140 150 m241 . pep LRILLAERIVGRQRHIDRIAGILTVQRLFHQRENAWTAVQIRNRFFGFVQKLIVGIIHL I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a241 LRILLAERIVGRKRHIDRIAGILTVQRLFHQRENAWTAVQIRNRFFGFVQKLIVGIIHL 190 200 210 220 230 240 160 170 m241.pep IMQRNHGIFHDSHICPFRNSRLITGAFX I I I I I I I I : I I I I I I I I I I I I I I I I I I I a241 IMQRNHGILHDSHICPFRNSRLITGAFX 250 260 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 911>: g241-l.seq 1 ATGATAGAAG TCATACATTT CTTCGGCACC GAAACGCGCA GACAGTTTGC 51 TTGTGCCGAC GTTGGACGAT TTCTGCATGA TGCCGCGCAC ATCCAAAGAG 101 GGGTAAACAT GGGTATCGCG CACGGGAGAC GGTCCGATTT TATAAGGCTG 151 CGTATTCAGC CGTTCGTTCA AATCGGTTTT GCCCGCATCC AATGCCTTCG 201 CAATCACAAA CGGTTTGATT GCCGAACCGG GTTCGATCAT ATCGGTTACG 251 GCACGGTTGC GCCGCTGTTC GCTGTCTGCC CGGCCGGGCC TGTTGGGATC 301 GTAGGCGGGC GTATTGGCCA AGGCGAGGAT TTCCCCCGTG CGGGCATCCA 351 ArACCACCAC CGTTCCGGCT TTTGCCTGAT GGTATTCGAC CGCCTTGTTC 401 AACTCTTCAT AGGCCAAGGT CTGAATCCTC TGATCGAGGG AAAGGATGAT 451 GTCTTTGCCG TTTTTCGGGG CTTTAkTGCG CGGGGAGTCC AAGCTGTCCA 501 CAATATTGCC CTGCCGGTCC CGCAAAACGA CTTCCGCGCC GTCTTCGCCA 551 TGCAAGCTGT CTTCAAGCGA AAGTTCCAAA CCTTCCTGAC CTTTGCCGTC 601 AATATCGGTA AATCCGATGA CGTGTGCAAA CAGGTTGCCC ATCGGGTAAT 651 GGCGTTTTAA This corresponds to the amino acid sequence <SEQ ID 912; ORF 241-1. ng>: 1 MPTRPTRAAN PPTPTTWLQT AYCPRPPYRP PSVQTHTPHE PASSTCAAKS 51 ANRRENSHNA QPTYLLHPΞN KMPSETEQTL FRRHQIPPSC RQSVWMTVR 101 TVDMTVCDFL IGCIAHAFNR SFKADFHACQ RMVAVHHRLA VGNIGYTIDD 151 NIAGFRIVRF KHHTDLDFNR ERARIFNTDQ LRIMLTERIV GRKRHFDRIA 201 GILTVQRLFH QRENAWTAV QIRNRFFGFI QKLIVGIIHL IMQRNHGIFC 251 NSHICPFRNS RLITGAF* The following partial DNA sequence was identified in N meningitidis <SEQ ID 913>: m241-l.seq 1 ATGCCAACAC GTCCAACTCG CGCTGCAAAC CCTCCAACCC CGCCAACCTG 51 GCTTCAGACG GCATACTGCC CTCGTCCGCC ATATCGTCCG CCGTCCGTGC 101 AAACGCGTAC ACCGCGTGAA CCGGCTTCCT CAACCTGCGC GGCAAAATCA 151 GCGAACCGAC GGGAAAATTC TCATAATGCC CAACCGACAT ACCTTCTCCA 201 TCCATCAAAC AAAATGCCGT CTGAAACGGA ACAAACCCTT TTCAGACGGC 251 ATCAGATACC TCCAAGCTGC CGGCAATCAG TGGTGGTGAT GACCGTGCGG 301 GCCGTGGACA TGACCGTGTG CGATTTCCTC ATCGGATGCA TCGCGCACGC 351 TTTCAACTGT AGCCTTAAAG CGGATTTTCA TGCCTGCCAA AGGATGGTTG 401 CCGTCCACCA CCGCCTTGCC GTCGGCAACA TCGGTTACAC GATAGACGAC 451 AACATCGCCG GTTTCAGGAT CGTCGGCTTC AAACATCATG CCGACTTCGA 501 CTTCAACAGG GAACACGCCC GCATCTTCGA TACGGACCAA CTCCGGATCC 551 TGCTCGCCGA ACGCATCGTC GGGCGACAGC GCCACATCGA CCGTATCGCC 601 GGCATCCTTA CCGTGCAACG CCTCTTCCAC CAAAGGGAAA ATGCCGTCGT 651 AACCGCCGTG CAGATACGCA ATCGGTTCTT CGGTTTTGTC CAAAAGCTGA 701 TTGTTGGCAT CATACATCTC ATAATGCAGC GAAACCACGG AATTTTTCAC 751 GATAGCCATA TTTGTCCTTT CAGGAACAGC AGATTAATTA CAGGCGCATT 801 CTAA This corresponds to the amino acid sequence <SEQ ID 914; ORF 241-1>: m241-l.pep 1 MPTRPTRAAN PPTPPTWLQT AYCPRPPYRP PSVQTRTPRE PASSTCAAKS 51 ANRRENSHNA QPTYLLHPSN KMPSETEQTL FRRHQIPPSC RQSVWMTVR 101 AVDMTVCDFL IGCIAHAFNC SLKADFHACQ RMVAVHHRLA VGNIGYTIDD 151 NIAGFRIVGF KHHADFDFNR EHARIFDTDQ LRILLAERIV GRQRHIDRIA 201 GILTVQRLFH QRENAWTAV QIRNRFFGFV QKLIVGIIHL IMQRNHGIFH 251 DSHICPFRNS RLITGAF* m241-l/g241-l 93.3% identity in 267 aa overlap 10 20 30 40 50 60 m241-1. pep MPTRPTRAANPPTPPTWLQTAYCPRPPYRPPSVQTRTPREPAΞSTCAAKSANRRENΞHNA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I : I I I I I I I I I II I I I I I I I I I I g241 MPTRPTRAANPPTPTTWLQTAYCPRPPYRPPSVQTHTPHEPAΞSTCAAKSANRRENSHNA 10 20 30 40 50 60 70 80 90 100 110 120 Itι241-l . pep QPTYLLHPSNKMPSETEQTLFRRHQIPPSCRQSVWMTVRAVDMTVCDFLIGCIAHAFNC I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I g241 QPTYLLHPSNKMPSETEQTLFRRHQIPPSCRQSVVVMTVRTVDMTVCDFLIGCIAHAFNR 70 80 90 100 110 120 130 140 150 160 170 180 m241-1.pep ΞLKADFHACQRMVAVHHRLAVGNIGYTIDDNIAGFRIVGFKHHADFDFNREHARIFDTDQ I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I : I I I I I : I I I I :| I I g241 SFKADFHACQRMVAVHHRLAVGNIGYTIDDNIAGFRIVRFKHHTDLDFNRERARIFNTDQ 130 140 150 160 170 180 190 200 210 220 230 240 m241-1. pep LRILLAERIVGRQRHIDRIAGILTVQRLFHQRENAWTAVQIRNRFFGFVQKLIVGIIHL I I I : I : I I I I I I :l I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I g241 LRIMLTERIVGRKRHFDRIAGILTVQRLFHQRENAWTAVQIRNRFFGFIQKLIVGIIHL 190 200 210 220 230 240 250 260 m241-l .pep IMQRNHGIFHDSHICPFRNSRLITGAFX : I I I I I I I I I I I I I I I I I g241 IMQRNHGIFCNSHICPFRNSRLITGAFX 250 260 The following partial DNA sequence was identified in N meningitidis <SEQ LD 915>: a241-l.seq 1 ATGCCAACAC GTCCAACTCG CGCCGCAAAG CATCCAACCC CGCCAACCTG 51 GCTTCAGACG GCATACTGCC CTCGTCCGCC ATATCGTCCG CCGTCCGTGC 101 AAACGCATAC ACCGCATGAA CCGGCTTCCT CAACCTGCGC GGCAAAATCA 151 GCGAACCGAC GGGAAAATTT TCATAATGCC CAACCGACAT ACCTTCTCCA 201 TCCATCAAAC AAAATGCCGT CTGAAATGGA ACAAACCCTT TTCAGACGGC 251 ATCAGATACC TCCAAGCTGC CGGCAATCAG TGGTGGTGAT GACCGTGCGG 301 ACCGTGGACA TGACCGTGTG CGATTTCCTC ATCGGATGCA TCGCGCACAC 351 TTTCAACCGT AGCCTTAAAG CGGATTTTCA TGCCTGCCAA AGGATGGTTG 401 CCGTCCACCA CCGCCTTACC GTCGGCAACA TCGGTTACAC GATAGACGAC 451 AACATCGCCG GTTTCAGGAT CGTCGGCTTC AAACATCATG CCGACTTCGA 501 CTTCAACAGG GAACACGCCC GCATCTTCAA TACGGACCAA CTCCGGATCC 551 TGCTCGCCGA ACGCATCGTC GGGCGAAAGC GCCACATCGA CCGTATCGCC 601 GGCATCCTTA CCGTGCAACG CCTCTTCCAC CAAAGGGAAA ATGCCGTCGT 651 AACCGCCGTG CAGATACGCA ATCGGTTCTT CGGTTTTGTC CAAAAGCTGA 701 TTGTTGGCAT CATACATCTC ATAATGCAGC GAAACCACGG AATTCTTCAC 751 GATAGCCATA TTTGTCCTTT CAGGAACAGC AGATTAATTA CAGGCGCATT 801 CTAA This corresponds to the amino acid sequence <SEQ ID 916; ORF 241-1. a>: a241-l.pep 1 MPTRPTRAAK HPTPPTWLQT AYCPRPPYRP PSVQTHTPHE PASSTCAAKS 51 ANRRENFHNA QPTYLLHPSN KMPSEMEQTL FRRHQIPPSC RQSVWMTVR 101 TVDMTVCDFL IGCIAHTFNR SLKADFHACQ RMVAVHHRLT VGNIGYTIDD 151 NIAGFRIVGF KHHADFDFNR EHARIFNTDQ LRILLAERIV GRKRHIDRIA 201 GILTVQRLFH QRENAWTAV QIRNRFFGFV QKLIVGIIHL IMQRNHGILH 251 DSHICPFRNS RLITGAF* 241-l/a241-l 95.1% identity in 267 aa overlap 10 20 30 40 50 60 m2 1-1. pep MPTRPTRAANPPTPPTWLQTAYCPRPPYRPPSVQTRTPREPAΞSTCAAKSANRRENSHNA II I I I I I I I I I I I I I I I I I I I I I I : I I : I I I I I I I I I I I I I I I I I III a241 MPTRPTRAAKHPTPPTWLQTAYCPRPPYRPPΞVQTHTPHEPAΞSTCAAKSANRRENFHNA 10 20 30 40 50 60 70 80 90 100 110 120 m241-1. pep QPTYLLHPSNKMPSETEQTLFRRHQIPPSCRQSVWMTVRAVDMTVCDFLIGCIAHAFNC I I I I I I I I I I I I I I I I I I I I I I I I :| I I I I I I I I I I I I I I: I I a241 QPTYLLHPSNKMPSEMEQTLFRRHQIPPΞCRQSVWMTVRTVDMTVCDFLIGCIAHTFNR 70 80 90 100 110 120 130 140 150 160 170 180 m241-1. pep SLKADFHACQRMVAVHHRLAVGNIGYTIDDNIAGFRIVGFKHHADFDFNREHARIFDTDQ I I I I I I I I I I I I I I I I I I I :| I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I :| I I a241 SLKADFHACQRMVAVHHRLTVGNIGYTIDDNIAGFRIVGFKHHADFDFNREHARIFNTDQ 130 140 150 160 170 180 190 200 210 220 230 240 m241-1. pep LRILLAERIVGRQRHIDRIAGILTVQRLFHQRENAVVTAVQIRNRFFGFVQKLIVGIIHL I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a241 LRILLAERIVGRKRHIDRIAGILTVQRLFHQRENAWTAVQIRNRFFGFVQKLIVGIIHL 190 200 210 220 230 240 250 260 m241-l .pep IMQRNHGIFHDΞHICPFRNSRLITGAFX I I I I I I I I :| I I I I I I I I I I I I I I I I I I a241 IMQRNHGILHDSHICPFRNΞRLITGAFX 250 260 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 917>: g242. seq 1 atgatcggcg aacttgttgt tttgttcgtg atcgagcact tcaagcaacg 51 cgctggcggg atcgccccga aagtcgctgc ccaatttgtc gatttcgtcg 101 agcaggaaca acgggtttct tacgcctgct tttgccatat tctgcaaaat 151 cttgccgggc atagagccga tataggtacg gcggtgcccg cggatttcgc 201 tttcgtcgcg cacgccgccc aaggccatac ggacatattt ccgccccgtt 251 gctttggcga tggattcgcc caaagaggtt ttgcccacgc ccggagggcc 301 gaccaaacac agaatcggac ctttgagctt gtccatacgt ttttggacgg 351 cgaggtattc caaaatccgt tctttgactt tttccaggcc gtagtggtcg 401 gcatccagca ccagtccggc tttggcgatg tctttgctga cgcgggattt 451 tttcttccac ggcagtccga gcagggtgtc gatgtagttg cgtacgacgg 501 tggattcggc agacatcggc ggcatcattt tgagtttttt cagttcggac 551 aggcattttt cttccgcttc tttggtcata cccgcctttt tgatgcctgc 601 ctccaaggca tccagttcgc cgttttcgtc ttcttcgccc aattctttgt 651 gtatcgcttt aatctgttcg ttcagataat attcgcgttg ggatttttcc 701 atttggcgtt tgacgcgtcc gcgtatgcgt ttttcggcct gcataatgtc 751 gagttcggat tccagctttg ccagcaggaa ttccatccgt ttgccgattt 801 cgggaatctc caaaatctgt tggcgttgcg ccagtttcaa ctgcaaatgc 851 gctgcgaccg tatcggttag This corresponds to the amino acid sequence <SEQ ID 918; ORF 242.ng>: g242.pep 1 MIGELWLFV IEHFKQRAGG IAPKVAAQFV DFVEQEQRVS YACFCHILQN 51 LAGHRADIGT AVPADFAFVA HAAQGHTDIF PPRCFGDGFA QRGFAHARRA 101 DQTQNRTFEL VHTFLDGEVF QNPFFDFFQA VGIQHQSG FGDVFADAGF 151 FLPRQSEQGV D AYDGGFG RHRRHHFEFF QFGQAFFFRF FGHTRLFDAC 201 LQGIQFAVFV FFAQFFVYRF NLFVQIIFAL GFFHLAFDAS AYAFFGLHNV 251 EFGFQLCQQE FHPFADFGNL QNLLALRQFQ LQMRCDRIG* The following partial DNA sequence was identified in N meningitidis <SEQ ED 919>: m242. seq 1 ATGATCGGCA AACTTGTTGT TTTGTTCGGG ATCGAGCACT TCGAGCAACG 51 CGCTGGCGGG ATCGCCTCGG AAGTCGTTAC CCAATTTGTC GATTTCGTCG 101 AGCAGGAACA AGGGGTTTTT CACGCCGGCT TTTGCCATAT TCTGCAAAAT 151 CTTACCGGGC ATAGAGCCGA TATAGGTGCG GCGGTGTCCC CTGATTTCGC 201 TTTCGTCGCG CACGCCGCCC AAAGCCATGC GGACATATTT CCGCCCCGTT 251 GCTTTGGCGA TGGATTCGCC CAAAGAGGTT TTGCCCACGC CCGGAGGGCC 301 GACCAGGCAC AGAATCGGGC CTTTGAGTTT GTCCATACGT TTTTGGACGG 351 CGAGGTATTC CAAAATCCGT TCTTTGACTT TTTCCAGGCC GTAGTGGTCG 401 GCATCCAGCA CCAGTCCGGC TTTGGCGATG TCTTTGCTGA CGCGGGATTT 451 TTTCTTCCAC GGCAGCTCGA GCAAAGTGTC GATGTAGTTG CGTACGACGG 501 TGGATTCCGC AGACATCGGT GGCATCATTT TGAGCTTTTT CAGTTCGGAC 551 AGGCATTTTT CTTCCGCTTC TTTGGTCATA CCCGCCTTTT TGATATCTGC 601 TTCCAAGGCA TCCAGTTCGC CGTTTTCGTC TTCTTCGCCC AGTTCTTTGT 651 GTATCGCTTT AATCTGTTCG TTCAGATAAT ATTCGCGCTG GGATTTTTCC 701 ATTTGGCGTT TGACGCGTCC GCGTATGCGT TTTTCGGCCT GCATAATGTC 751 GAGTTCGGAT TCCAGCTGTG CCAGCAGGAA TTCCATCCGT TTGCCGATTT 801 CGGGAATTTC CAAAATCTGT TGGCGTTGCG CCAGTTTCAA CTGCAAATGC 851 GCTGCGACCG TATCGGTTAG This corresponds to the amino acid sequence <SEQ ID 920; ORF 242>: m242.pep 1 MIGKLWLFG IEHFEQRAGG IASEWTQFV DFVEQEQGVF HAGFCHILQΝ 51 LTGHRADIGA AVSPDFAFVA HAAQSHADIF PPRCFGDGFA QRGFAHARRA 101 DQAQΝRAFEF VHTFLDGEVF QNPFFDFFQA WVGIQHQSG FGDVFADAGF 151 FLPRQLEQSV DWAYDGGFR RHRWHHFELF QFGQAFFFRF FGHTRLFDIC 201 FQGIQFAVFV FFAQFFVYRF NLFVQIIFAL GFFHLAFDAS AYAFFGLHNV 251 EFGFQLCQQE FHPFADFGNF QNLLALRQFQ LQMRCDRIG* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 242 shows 90.3% identity over a 289 aa overlap with a predicted ORF (ORF 242.ng) from N gonorrhoeae: m242/g24290.3% identity in 289 aa overlap 10 20 30 40 50 60 m242.pep MIGKLWLFGIEHFEQRAGGIASEWTQFVDFVEQEQGVFHAGFCHILQΝLTGHRADIGA M h l l l M 1 1 1 1 1 1 1 1 1 1 - - M M ! I M g242 MIGELWLFVIEHFKQRAGGIAPKVAAQFVDFVEQEQRVSYACFCHILQΝLAGHRADIGT 10 20 30 40 50 60 70 80 90 100 110 120 m242.pep AVSPDFAFVAHAAQSHADIFPPRCFGDGFAQRGFAHARRADQAQΝRAFEFVHTFLDGEVF I I g242 AVPADFAFVAHAAQGHTDIFPPRCFGDGFAQRGFAHARRADQTQΝRTFELVHTFLDGEVF 70 80 90 100 110 120 130 140 150 160 170 180 m242.pep QNPFFDFFQAVWGIQHQSGFGDVFADAGFFLPRQLEQSVDWAYDGGFRRHRWHHFELF MMMMMMMMMMMMMMIMMM IhlMMIMM III Mlhl g242 QNPFFDFFQAVWGIQHQSGFGDVFADAGFFLPRQSEQGVDWAYDGGFGRHRRHHFEFF 130 140 150 160 170 180 190 200 210 220 230 240 m242.pep QFGQAFFFRFFGHTRLFDICFQGIQFAVFVFFAQFFVYRFNLFVQIIFALGFFHLAFDAS MMMMMMMMM h I II II II 1111 II II 11 II I II 11 II I II II 111 II II I g242 QFGQAFFFRFFGHTRLFDACLQGIQFAVFVFFAQFFVYRFNLFVQIIFALGFFHLAFDAS 190 200 210 220 230 240 250 260 270 280 290 π\242.pep AYAFFGLHNVEFGFQLCQQEFHPFADFGNFQNLLALRQFQLQMRCDRIGX Mill II MM Mill Mill III III MM III MM 111111111111 g242 AYAFFGLHNVEFGFQLCQQEFHPFADFGNLQNLLALRQFQLQMRCDRIGX 250 260 270 280 The following partial DNA sequence was identified in N meningitidis <SEQ ID 921>: a242. seq 1 ATGATCGGCG AACTTGTTGT TTTGCTCGGG ATCAAGCACT TCGAGCAACG 51 CGCTGGCGGG ATCGCCCCGG AAGTCGCTAΝ CCAATTTGTC GATTTCGTCG 101 AGCAGGAACA ATGGGTTTTT TACGCCGGCT TTTGCCATAT TCTGCAAAAT 151 CTTACCGGGC ATGGAGCCGA TATAGGTGCG GCGGTGTCCC CGGATTTCGC 201 TTTCGTCGCG CACGCCGCCC AAAGCCATGC GGACATATTT CCGCCCCGTT 251 GCTTTGGCGA TGGATTCGCC CAAAGAGGTT TTGCCCACGC CTGGAGGGCC 301 GACCAGGCAC AGAATCGGGC CTTTGAGTTT GTCCATACGT TTTTGGACGG 351 CGAGGTATTC CAAAATCCGT TCTTTGACTT TTTCCAGGCC GTAGTGGTCG 401 GTATCCAGCA CCAATCCGGC TTTGGCGATG TCTTTGCTGA CGCGGGATTT 451 TTTCTTCCAC GGCAGTTCGA GCAGGGTGTC GATGTAGTTG CGTACGACGG 501 TGGATTCGGC AGACATCGGC GGCATCATTT TGAGCTTTTT CAGTTCGGAC 551 AGGCATTTTT CTTCCGCTTC TTTGGTCATA CCCGCCTTTT TGATATCTGC 601 TTCCAAGGCA TCCAGTTCGC CGTTTTCGTC TTCTTCGCCC AGTTCTTTGT 651 GTATCGCTTT AATCTGTTCG TTCAGATAAT ATTCGCGCTG GGATTTTTCC 701 ATTTGGCGTT TGACGCGTCC GCGTATGCGT TTTTCGGCCT GCATAATGTC 751 GAGTTCGGAT TCCAGCTGTG CCAGCAGGAA TTCCATCCGT TTGCCGATTT 801 CGGGAATTTC CAAAATCTGT TGGCGTTGCG CCAGTTTCAA CTGCAAATGC 851 GCTGCGACCG TATCGGTTAG This corresponds to the amino acid sequence <SEQ ID 922; ORF 242. a>: a242.pep 1 MIGELVVLLG IKHFEQRAGG IAPEVAXQFV DFVEQEQWVF YAGFCHILQΝ 51 LTGHGADIGA AVSPDFAFVA HAAQSHADIF PPRCFGDGFA QRGFAHAWRA 101 DQAQΝRAFEF VHTFLDGEVF QNPFFDFFQA WVGIQHQSG FGDVFADAGF 151 FLPRQFEQGV DWAYDGGFG RHRRHHFELF QFGQAFFFRF FGHTRLFDIC 201 FQGIQFAVFV FFAQFFVYRF NLFVQIIFAL GFFHLAFDAS AYAFFGLHNV 251 EFGFQLCQQE FHPFADFGNF QNLLALRQFQ LQMRCDRIG* m242/a242 95.2% identity in 289 aa overlap 10 20 30 40 50 60 m242. pep MIGKLWLFGIEHFEQRAGGIASEWTQFVDFVEQEQGVFHAGFCHILQNLTGHRADIGA I I I : II I I : I I : I I I I I I I I I I I I :: I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I a242 MIGELWLLGIKHFEQRAGGIAPEVAXQFVDFVEQEQWVFYAGFCHILQNLTGHGADIGA 10 20 30 40 50 60 70 80 90 100 110 120 m242. pep AVSPDFAFVAHAAQSHADIFPPRCFGDGFAQRGFAHARRADQAQNRAFEFVHTFLDGEVF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a242 AVSPDFAFVAHAAQSHADIFPPRCFGDGFAQRGFAHAWRADQAQNRAFEFVHTFLDGEVF 70 80 90 100 110 120 130 140 150 160 170 180 m242. pep QNPFFDFFQAVWGIQHQSGFGDVFADAGFFLPRQLEQSVDWAYDGGFRRHRWHHFELF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I: I I I I I I I I I I I I I l l l l l l a242 QNPFFDFFQAVWGIQHQSGFGDVFADAGFFLPRQFEQGVDWAYDGGFGRHRRHHFELF 130 140 150 160 170 180 190 200 210 220 230 240 m242 . pep QFGQAFFFRFFGHTRLFDICFQGIQFAVFVFFAQFFVYRFNLFVQIIFALGFFHLAFDAS II I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a242 QFGQAFFFRFFGHTRLFDICFQGIQFAVFVFFAQFFVYRFNLFVQIIFALGFFHLAFDAS 190 200 210 220 230 240 250 260 270 280 290 m242. pep AYAFFGLHNVEFGFQLCQQEFHPFADFGNFQNLLALRQFQLQMRCDRIGX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a242 AYAFFGLHNVEFGFQLCQQEFHPFADFGNFQNLLALRQFQLQMRCDRIGX 250 260 270 280 290 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 923>: g243.seq 1 ATGGTaatcg tctGGTTGCc cgAGTTaccg CCGATGCCGG CGACGATGGG 51 CATCAGCGCG GCGAGTGCGA CGATTTTTTC gatactgcCT TCAAACGCGC 101 CGATGACGCG GCTGGCGAGG AAGGCGGTGC AGAGGTTGAC GGCGAGCCAC 151 ATCCAGCGGT TTTTGACGGA ATCCAAGACG GGGGCGAACA GGTCTTCCTC 201 TTCCTGCAAA CCTGCCATGT TCAACATATC CGCTTCGGAT TCTTCGCGGA 251 TCACGTCCAC CATCTCGTCG ATGGTAATCc tgCCGATGAG CTTTTTGTTT 301 TCATCAACGA CGGGCGCGGT AACCAAGTCG TAG This corresponds to the amino acid sequence <SEQ ID 924; ORF 243. ng>: g243.pep 1 MVIVWLPELP PMPATMGISA ASATIFSILP SΝAPMTRLAR KAVQRLTASH 51 IQRFLTESKT GAΝRSSSSCK PAMFΝISASD SSRITSTISS MVILPMSFLF 101 SSTTGAVTKS * The following partial DΝA sequence was identified in N meningitidis <SEQ ID 925>: m243. seq 1 ATGGTAATCG TCTGGTTGCC CGAGTTACCG CCTATGCCGG CGACGATGGG 51 CATCAGCGCG GyGAGTGCGA CGATTTTTTC GATGCTGCCT TCAAACGCGC 101 CGATAACACG GyTGGCGAGG AAGGCGGTGC AGAGGTTGAC GGCGAGCCAC 151 ATCCAGyGGT TTTTCACCGA ATCCCACACG GGGGCGAAyA GGTCTTCCTC 201 TTCCTGCAAA CCCGCCATAT TCAGCATATC CGCTTCCGAT TCTTCGCGGA 251 TCACGTCCAC CATCTCGTCG ATGGTAATCC TGCCGATGAG CTTTTTGTTT 301 TCATCGACGA CGGGCGCGGT AACCAAGTCG TAG This corresponds to the amino acid sequence <SEQ ID 926; ORF 243>: m243.pep 1 MVIVWLPELP PMPATMGISA XSATIFSMLP SΝAPITRLAR KAVQRLTASH 51 IQXFFTESHT GAΝRSSSSCK PAIFSISASD SSRITSTISS MVILPMSFLF 101 SSTTGAVTKS * Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 243 shows 92.7% identity over a 110 aa overlap with a predicted ORF (ORF 243.ng) from N gonorrhoeae: m243/g243 10 20 30 40 50 60 m243. pep MVIVWLPELPPMPATMGISAXSATIFSMLPSΝAPITRLARKAVQRLTASHIQXFFTESHT M M M M M M M M M M I I 1 1 1 h I I I I I h I I 1 1 I I 1 1 1 I I I I 1 1 I I h l l h l g243 MVIVWLPELPPMPATMGISAASATIFSILPSΝAPMTRLARKAVQRLTASHIQRFLTESKT 10 20 30 40 50 60 70 80 90 100 110 m243.pep GAΝRSSSSCKPAIFSISASDSSRITSTISSMVILPMSFLFSSTTGAVTKSX MMMMMIhhMMMMMMMMMMMMMMMMMM g243 GAΝRSSSSCKPAMFΝISASDSSRITSTISSMVILPMΞFLFSSTTGAVTKSX 70 80 90 100 110 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 927>: a243. seq 1 ATGGTAATCG TCTGGTTGCC CGAGTTACCG CCTATGCCGG CGACGATGGG 51 CATCAGCGCG GCGAGTGCGA CGATTTTTTC GATGCTGCCT TCAAACGCGC 101 CGATAACACG GCTGGCGAGG AAGGCGGTGC AGAGGTTGAC GGCGAGCCAC 151 ATCCAGCGGT TTTTGACGGA ATCCAAGACG GGGGCGAATA AGTCTTCCTC 201 TTCTTGCAAA CCCGCCATAT TCAACATATC CGCTTCGGAT TCTTCGCGGA 251 TCACGTCCAC CATTTCGTCA ACGGTCACCC TGCCGATGAG CTTTTTGTTT 301 TCATCGACGA CGGGCGCGGT AACCAAGTCA TAG This corresponds to the amino acid sequence <SEQ ID 928; ORF 243. a>: a243.pep 1 MVIVWLPELP PMPATMGISA ASATIFSMLP SΝAPITRLAR KAVQRLTASH 51 IQRFLTESKT GAΝKSSSSCK PAIFΝISASD SSRITSTISS TVTLPMSFLF 101 SSTTGAVTKS * m243/a243 92.7% identity in 110 aa overlap 10 20 30 40 50 60 m243 . pep MVIVWLPELPPMPATMGISAXSATIFSMLPSΝAPITRLARKAVQRLTASHIQXFFTESHT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I l l l a243 MVIVWLPELPPMPATMGISAASATIFSMLPSΝAPITRLARKAVQRLTASHIQRFLTESKT 10 20 30 40 50 60 70 80 90 100 110 m243.pep GAΝRSSSSCKPAIFSISASDSSRITSTISSMVILPMSFLFSSTTGAVTKSX I I I : I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a2 3 GAΝKSSSSCKPAIFΝISASDSSRITSTISSTVTLPMSFLFSSTTGAVTKSX 70 80 90 100 110 The following partial DΝA sequence was identified in N. gonorrhoeae <SEQ ID 929>: g244. seq 1 atgccgcctg aagcccggcc ggcgggttca gacggcattg ccgctttact 51 tcgatcggtt tatacgcaaa acgcgcttca ggaaataaat cagattattc 101 cccagacgcc ttcaggcttc cttccgtgcc accgtaacca tagccgggcg 151 caacacacgg tcggacaggg tataaccctt cttcatcaca ccaaccacgg 201 tattgggttc ctgctcactg gccaccgcct gcatcgcctg atggatattc 251 ggatcgagct tatcgcccgc tttaggattg atttccttga tttgcgtggc 301 atcaaacgcc ttctgcaact cattcaaagt catctgcaca cccattttca 351 gcgcatcgaa attaccgctc tgatccaaaa gcgccatttc cagataatcc 401 ttgaccggca acatttccac ggcaaacttc tgtccggcga acttgtgcgt 451 atcggcaatt tcctgctggt ggcggcggcg caggttttgc tcgtttgcca 501 aagcgcgcag ttgttcgtct ttcaactgcg cttccagctc ggcaatccgc 551 gcctgcaaat cctcataagc cggctcggcg gcagcctgtt cctgtacacc 601 gtccgcattt cctactgtct cgacggtttc caccgcctcc acattttcaa 651 ccgcttcttc actgttttgc tgctgtgtct gttcgctcat atcgtatccc 701 tcaaaacaaa ttggaaatca aaatccggtt attacccgag caagataagg 751 acattttcaa gaaacttcaa gcaaaggcag gaaatttcac atccgccgcc 801 gaatacccta ccgcaaaaac catataaacg gtaa This corresponds to the amino acid sequence <SEQ ID 930; ORF 244.ng>: g244.pep 1 MPPEARPAGS DGIAALLRSV YTQΝALQEIΝ QIIPQTPSGF LPCHRΝHSRA 51 QHTVGQGITL LHHTΝHGIGF LLTGHRLHRL MDIRIELIAR FRIDFLDLRG 101 IKRLLQLIQS HLHTHFQRIE ITALIQKRHF QIILDRQHFH GKLLSGELVR 151 IGΝFLLVAAA QVLLVCQSAQ LFVFQLRFQL GΝPRLQILIS RLGGSLFLYT 201 VRISYCLDGF HRLHIFΝRFF TVLLLCLFAH IVSLKTΝWKS KSGYYPSKIR 251 TFSRΝFKQRQ EISHPPPΝTL PQKPYKR* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 931>: m244 . seq 1 ATGCCGTCTG AAGCCCGACA GGCGGGTTCA GACGGCATTG CCGCTTTACT 51 TCGATCGGTT TATACGCAAA ACGCGCTTCA GGAAATAAAT CAGATTATTC 101 CCCAGACGCC TTCAGGCTTC CTTCTGCGCC ACCGTAACCA TAGCCGGGCG 151 CAACACGCGG TCGGACAGCG TATAACCCTT CTTCATCACA CCCACCACGG 201 TATTCGGCTC CTGTTCGCTT GCCACCGCCT GCATCGCCTG ATGGATATTC 251 GGATCGAGCT TATCGCCCGC TTTAGGGTTG ATTTCCTTGA TTTGCGTAGC 301 ATCAAATGCT TTCTGCAACT CGTTCAAAGT CATCTGCACG CCCATTTTCA 351 GCGCATCGAA ATTGCCGCTC TGATCCAAAA GCGCCATTTC CAGATAATCC 401 TTGACCGGCA GCATTTCCAC GGCAAACTTC TGTCCGGCGA ACTTGTGCGT 451 ATCCGCAATT TyCTGCTGGT GGCGGCGGCG CAGGTTTTGC TCGTTTGCCA 501 AAGCGCGCTG CTCGTCTTTC AACTGCGTTT CCAGCTCGGC AATCCGCGCC 551 TGCAAATCCT CATAAGCCGG CTCTGCGGCA GCCTGTTCCT GCACACCGTC 601 CGCATTTCCT ACTGTTTCGA CGGTTTCCAC CGCCTCCACA TTTTCAACCG 651 CTTCTTCACT GTTTTGCTGC TGTGTCTGTT CGCTCATATC GTATCCCTTA 701 AAACAAATTG GAAATCAAAA TCCAGTTATT ACCCGCGCAA GATAAGGACA 751 TTTTCAAGAA ACTTCAAkCA AAAkCAGAGA ATTTCAAATT CATTTTCAAA 801 TCCCCTACCG AAAAAATAAT ATAGACGGTA A This corresponds to the amino acid sequence <SEQ ID 932; ORF 244>: m244.pep 1 MPSEARQAGS DGIAALLRSV YTQNALQEIN QIIPQTPSGF LLRHRNHSRA 51 QHAVGQRITL LHHTHHGIRL LFACHRLHRL MDIRIELIAR FRVDFLDLRS 101 IKCFLQLVQS HLHAHFQRIE IAALIQKRHF QIILDRQHFH GKLLSGELVR 151 IRNFLLVAAA QVLLVCQSAL LVFQLRFQLG NPRLQILISR LCGSLFLHTV 201 RISYCFDGFH RLHIFNRFFT VLLLCLFAHI VSLKTNWKSK SSYYPRKIRT 251 FSRNFXQXQR ISNSFSNPLP KKXYRR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 244 shows 86.3% identity over a 277 aa overlap with a predicted ORF (ORF 244.ng) from N gonorrhoeae: M244/G244 10 20 30 40 50 60 m244.pep MPSEARQAGSDGIAALLRSVYTQΝALQEIΝQIIPQTPSGFLLRHRΝHSRAQHAVGQRITL I I I I I M M M M M M M M M M M M M M M M M M l g244 MPPEARPAGSDGIAALLRSVYTQΝALQEIΝQIIPQTPSGFLPCHRΝHSRAQHTVGQGITL 10 20 30 40 50 60 70 80 90 100 110 120 m244. pep LHHTHHGIRLLFACHRLHRLMDIRIELIARFRVDFLDLRSIKCFLQLVQSHLHAHFQRIE l l l h l l l M = = 1 1 1 1 1 1 1 M 1 1 1 1 1 1 1 1 1 : 1 1 1 1 1 1 = 1 1 g244 LHHTΝHGIGFLLTGHRLHRLMDIRIELIARFRIDFLDLRGIKRLLQLIQSHLHTHFQRIE 70 80 90 100 110 120 130 140 150 160 170 180 m244.pep IAALIQKRHFQIILDRQHFHGKLLSGELVRIRΝFLLVAAAQVLLVCQSAALLVFQLRFQL M M M M M M M M ! I M M ! M l 1 1 1 1 1 1 I I I I I I 1 1 I I I g244 ITALIQKRHFQIILDRQHFHGKLLSGELVRIGΝFLLVAAAQVLLVCQSAQLFVFQLRFQL 130 140 150 160 170 180 190 200 210 220 230 240 m244. pep GΝPRLQILISRLCGSLFLHTVRISYCFDGFHRLHIFΝRFFTVLLLCLFAHIVSLKTΝWKS III I g244 GΝPRLQILISRLGGSLFLYTVRISYCLDGFHRLHIFΝRFFTVLLLCLFAHIVSLKTΝWKS 190 200 210 220 230 240 250 260 270 m244.pep KSSYYPRKIRTFSRΝFXQXQRISΝSFSΝPLPKKXYRRX I h l l l I h l h I M M h l l g244 KSGYYPSKIRTFSRΝFKQRQEISHPPPΝTLPQKPYKRX 250 260 270 The following partial DNA sequence was identified in N meningitidis <SEQ ID 933>: a244 . seq 1 ATGCCGTCTG AAGCCCGACA GGCGGGTTCA GACGGCATTG CCGCTTTACT 51 TCGATCGGTT TATACGCAAA ACGCGCTTCA GGAAATAAAT CAGATTATTC 101 CCCAGACGCC TTCAGGCTTC CTTCTGTGCC ACCGTAACCA TAGCCGGGCG 151 CAACACGCGG TCGGACAGCG TATAACCCTT CTTCATCACG CCCACCACGG 201 TATTGGGTTC CTGTTCGCTT GCCACCGCCT GCATCGCCTG ATGGATATTC 251 GGATCGAGCT TATCGCCCGC TTTAGGATTG ATTTCCTTGA TTTGCGTAGC 301 ATCAAATGCT TTCTGCAACT CGTTCAAAGT CATCTGCACG CCCATTTTCA 351 GCGCATCGAA ATTGCCGCTC TGATCCAAAA GCGCCATTTC CAGATAATCC 401 TTGACCGGCA GCATTTCCAC GGCAAACTTC TGTCCGGCGA ACTTGTGCGT 451 ATCCGCAATT TCCTGCTGGT GGCGGCGGCG CAGGTTTTGC TCGTTTGCCA 501 AAGCGCGCAG CTGCTCGTCT TTCAACTGCG CTTCCAGCTC GGCAATCCGC 551 GCCTGCAAAT CCTCATAAGC CGGCTCTGCG GCAGCCTGTT CCTGCACACC 601 GTCCGCATTT CCTACTGTCT CGACGGTTTC CACCGCCTCC ACATTTTCAA 651 CCGCTTCTTC ACTGTTTTGC TGCTGTGTCT GTTCGCTCAT ATCGTATCCC 701 TTAAAACAAA TTGGAAATCA AAATCCAGTT ATTACCCGCG CAAGATAAGG 751 ACATTTTCAA GAAACTTCAA GCAAAGGCAG AGAATTTCAA ATTCATTTTC 801 AAATCCCCTA CCGAAAAAAT AATATAGACG GTAA This corresponds to the amino acid sequence <SEQ ID 934; ORF 244. a>: a24 .pep 1 MPSEARQAGS DGIAALLRSV YTQΝALQEIΝ QIIPQTPSGF LLCHRΝHSRA 51 QHAVGQRITL LHHAHHGIGF LFACHRLHRL MDIRIELIAR FRIDFLDLRS 101 IKCFLQLVQS HLHAHFQRIE IAALIQKRHF QIILDRQHFH GKLLSGELVR 151 IRΝFLLVAAA QVLLVCQSAQ LLVFQLRFQL GΝPRLQILIS RLCGSLFLHT 201 VRISYCLDGF HRLHIFΝRFF TVLLLCLFAH IVSLKTΝWKS KSSYYPRKIR 251 TFSRΝFKQRQ RISΝSFSΝPL PKK*YRR* m244/a244 96.8% identity in 277 aa overlap 10 20 30 40 50 60 m244. pep MPSEARQAGSDGIAALLRSVYTQΝALQEIΝQIIPQTPSGFLLRHRΝHSRAQHAVGQRITL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a244 MPSEARQAGSDGIAALLRSVYTQΝALQEIΝQIIPQTPSGFLLCHRΝHSRAQHAVGQRITL 10 20 30 40 50 60 70 80 90 100 110 120 m244 . pep LHHTHHGIRLLFACHRLHRLMDIRIELIARFRVDFLDLRSIKCFLQLVQSHLHAHFQRIE l l l l l l : I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I a24 LHHAHHGIGFLFACHRLHRLMDIRIELIARFRIDFLDLRSIKCFLQLVQSHLHAHFQRIE 70 80 90 100 110 120 130 140 150 160 170 179 m244.pep IAALIQKRHFQIILDRQHFHGKLLSGELVRIRΝFLLVAAAQVLLVCQSA-LLVFQLRFQL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II lllllllll! a244 IAALIQKRHFQIILDRQHFHGKLLSGELVRIRΝFLLVAAAQVLLVCQSAQLLVFQLRFQL 130 140 150 160 170 180 180 190 200 210 220 230 239 m244.pep GΝPRLQILISRLCGSLFLHTVRISYCFDGFHRLHIFΝRFFTVLLLCLFAHIVSLKTΝWKS I I I I I II I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a244 GΝPRLQILISRLCGSLFLHTVRISYCLDGFHRLHIFΝRFFTVLLLCLFAHIVSLKTΝWKS 190 200 210 220 230 240 240 250 260 270 m244.pep KSSYYPRKIRTFSRΝFXQXQRISΝSFSΝPLPKKXYRRX I I I I I I I I I I I 1 I I I I I I I I I I I I I I I I I I I I I I a244 KSSYYPRKIRTFSRΝFKQRQRISΝSFSΝPLPKKXYRRX 250 260 270 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 935>: g244-l.seq 1 atgccgcctg aagcccggcc ggcgggttca gacggcattg ccgctttact 51 tcgatcggtt tatacgcaaa acgcgcttca ggaaataaat cagattattc 101 cccagacgcc ttcaggcttc cttccgtgcc accgtaacca tagccgggcg 151 caacacacgg tcggacaggg tataaccctt cttcatcaca ccaaccacgg 201 tattgggttc ctgctcactg gccaccgcct gcatcgcctg atggatattc 251 ggatcgagct tatcgcccgc tttaggattg atttccttga tttgcgtggc 301 atcaaacgcc ttctgcaact cattcaaagt catctgcaca cccattttca 351 gcgcatcgaa attaccgctc tgatccaaaa gcgccatttc cagataatcc 401 ttgaccggca acatttccac ggcaaacttc tgtccggcga acttgtgcgt 451 atcggcaatt tcctgctggt ggcggcggcg caggttttgc tcgtttgcca 501 aagcgcgcag ttgttcgtct ttcaactgcg cttccagctc ggcaatccgc 551 gcctgcaaat cctcataagc cggctcggcg gcagcctgtt cctgtacacc 601 gtccgcattt cctactgtct cgacggtttc caccgcctcc acattttcaa 651 ccgcttcttc actgttttgc tgctgtgtct gttcgctcat atcgtatccc 701 tcaaaacaaa ttggaaatca aaatccggtt attacccgag caagataagg 751 acattttcaa gaaacttcaa gcaaaggcag gaaatttcac atccgccgcc 801 gaatacccta ccgcaaaaac catataaacg gtaa This corresponds to the amino acid sequence <SEQ ID 936; ORF 244-1. ng>: g244-l.pep 1 MPPEARPAGS DGIAALLRSV YTQNALQEIN QIIPQTPSGF LPCHRNHSRA 51 QHTVGQGITL LHHTNHGIGF LLTGHRLHRL MDIRIELIAR FRIDFLDLRG 101 IKRLLQLIQS HLHTHFQRIE ITALIQKRHF QIILDRQHFH GKLLSGELVR 151 IGNFLLVAAA QVLLVCQSAQ LFVFQLRFQL GNPRLQILIS RLGGSLFLYT 201 VRISYCLDGF HRLHIFNRFF TVLLLCLFAH IVSLKTNWKS KSGYYPSKIR 251 TFSRNFKQRQ EISHPPPNTL PQKPYKR* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 937>: m244-l.seq 1 ATGCCGTCTG AAGCCCGACA GGCGGGTTCA GACGGCATTG CCGCTTTACT 51 TCGATCGGTT TATACGCAAA ACGCGCTTCA GGAAATAAAT CAGATTATTC 101 CCCAGACGCC TTCAGGCTTC CTTCTGCGCC ACCGTAACCA TAGCCGGGCG 151 CAACACGCGG TCGGACAGCG TATAACCCTT CTTCATCACA CCCACCACGG 201 TATTCGGCTC CTGTTCGCTT GCCACCGCCT GCATCGCCTG ATGGATATTC 251 GGATCGAGCT TATCGCCCGC TTTAGGGTTG ATTTCCTTGA TTTGCGTAGC 301 ATCAAATGCT TTCTGCAACT CGTTCAAAGT CATCTGCACG CCCATTTTCA 351 GCGCATCGAA ATTGCCGCTC TGATCCAAAA GCGCCATTTC CAGATAATCC 401 TTGACCGGCA GCATTTCCAC GGCAAACTTC TGTCCGGCGA ACTTGTGCGT 451 ATCCGCAATT TyCTGCTGGT GGCGGCGGCG CAGGTTTTGC TCGTTTGCCA 501 AAGCGCGCTG CTCGTCTTTC AACTGCGTTT CCAGCTCGGC AATCCGCGCC 551 TGCAAATCCT CATAAGCCGG CTCTGCGGCA GCCTGTTCCT GCACACCGTC 601 CGCATTTCCT ACTGTTTCGA CGGTTTCCAC CGCCTCCACA TTTTCAACCG 651 CTTCTTCACT GTTTTGCTGC TGTGTCTGTT CGCTCATATC GTATCCCTTA 701 AAACAAATTG GAAATCAAAA TCCAGTTATT ACCCGCGCAA GATAAGGACA 751 TTTTCAAGAA ACTTCAAkCA AAAkCAGAGA ATTTCAAATT CATTTTCAAA 801 TCCCCTACCG AAAAAATAA This corresponds to the amino acid sequence <SEQ ID 938; ORF 244- 1>: m244-l.pep 1 MPSEARQAGS DGIAALLRSV YTQΝALQEIΝ QIIPQTPSGF LLRHRΝHSRA 51 QHAVGQRITL LHHTHHGIRL LFACHRLHRL MDIRIELIAR FRVDFLDLRS 101 IKCFLQLVQS HLHAHFQRIE IAALIQKRHF QIILDRQHFH GKLLSGELVR 151 IRΝFLLVAAA QVLLVCQSAL LVFQLRFQLG ΝPRLQILISR LCGSLFLHTV 201 RISYCFDGFH RLHIFΝRFFT VLLLCLFAHI VSLKTΝWKSK SΞYYPRKIRT 251 FSRΝFXQXQR ISΝSFSΝPLP KK* m244-l/G244-l 86.3% identity in 277 aa overlap 10 20 30 40 50 60 m244-l.pep MPSEARQAGSDGIAALLRSVYTQΝALQEIΝQIIPQTPSGFLLRHRΝHSRAQHAVGQRITL I I I I I I I I g244-l MPPEARPAGSDGIAALLRSVYTQΝALQEIΝQIIPQTPSGFLPCHRΝHSRAQHTVGQGITL 10 20 30 40 50 60 70 80 90 100 110 120 m244 -1. pep LHHTHHGIRLLFACHRLHRLMDIRIELIARFRVDFLDLRSIKCFLQLVQSHLHAHFQRIE l l l h l l l ■■ ] ■■ ■■ M I I I I M I I I I I M I I h l l l l l h l l I h 1 1 I I h 1 1 1 1 1 1 g244-l LHHTΝHGIGFLLTGHRLHRLMDIRIELIARFRIDFLDLRGIKRLLQLIQSHLHTHFQRIE 70 80 90 100 110 120 130 140 150 160 170 180 m244 -1. pep IAALIQKRHFQIILDRQHFHGKLLSGELVRI NFLLVAAAQVLLVCQSAALLVFQLRFQL g244 -1 ITALIQKRHFQIILDRQHFHGKLLSGELVRIGNFLLVAAAQVLLVCQSAQLFVFQLRFQL 130 140 150 160 170 180 190 200 210 220 230 240 m244-l.pep GNPRLQILISRLCGSLFLHTVRISYCFDGFHRLHIFNRFFTVLLLCLFAHIVSLKTNWKS g244 -1 GNPRLQILISRLGGSLFLYTVRISYCLDGFHRLHIFNRFFTVLLLCLFAHIVSLKTNWKS 190 200 210 220 230 240 250 260 270 244-l .pep KSSYYPRKIRTFSRNFXQXQRISNSFSNPLPKKX I h l l l I M M I M g244 - 1 KSGYYPSKIRTFSRNFKQRQEISHPPPNTLPQKPYKRX 250 260 270 The following partial DNA sequence was identified in N meningitidis <SEQ ID 939>: a244-l . seq 1 ATGCCGTCTG AAGCCCGACA GGCGGGTTCA GACGGCATTG CCGCTTTACT 51 TCGATCGGTT TATACGCAAA ACGCGCTTCA GGAAATAAAT CAGATTATTC 101 CCCAGACGCC TTCAGGCTTC CTTCTGTGCC ACCGTAACCA TAGCCGGGCG 151 CAACACGCGG TCGGACAGCG TATAACCCTT CTTCATCACG CCCACCACGG 201 TATTGGGTTC CTGTTCGCTT GCCACCGCCT GCATCGCCTG ATGGATATTC 251 GGATCGAGCT TATCGCCCGC TTTAGGATTG ATTTCCTTGA TTTGCGTAGC 301 ATCAAATGCT TTCTGCAACT CGTTCAAAGT CATCTGCACG CCCATTTTCA 351 GCGCATCGAA ATTGCCGCTC TGATCCAAAA GCGCCATTTC CAGATAATCC 401 TTGACCGGCA GCATTTCCAC GGCAAACTTC TGTCCGGCGA ACTTGTGCGT 451 ATCCGCAATT TCCTGCTGGT GGCGGCGGCG CAGGTTTTGC TCGTTTGCCA 501 AAGCGCGCAG CTGCTCGTCT TTCAACTGCG CTTCCAGCTC GGCAATCCGC 551 GCCTGCAAAT CCTCATAAGC CGGCTCTGCG GCAGCCTGTT CCTGCACACC 601 GTCCGCATTT CCTACTGTCT CGACGGTTTC CACCGCCTCC ACATTTTCAA 651 CCGCTTCTTC ACTGTTTTGC TGCTGTGTCT GTTCGCTCAT ATCGTATCCC 701 TTAAAACAAA TTGGAAATCA AAATCCAGTT ATTACCCGCG CAAGATAAGG 751 ACATTTTCAA GAAACTTCAA GCAAAGGCAG AGAATTTCAA ATTCATTTTC 801 AAATCCCCTA CCGAAAAAAT AA This corresponds to the amino acid sequence <SEQ ID 940; ORF 244-l.a>: a244-l.pep 1 MPSEARQAGS DGIAALLRSV YTQΝALQEIΝ QIIPQTPSGF LLCHRΝHSRA 51 QHAVGQRITL LHHAHHGIGF LFACHRLHRL MDIRIELIAR FRIDFLDLRS 101 IKCFLQLVQS HLHAHFQRIE IAALIQKRHF QIILDRQHFH GKLLSGELVR 151 IRΝFLLVAAA QVLLVCQSAQ LLVFQLRFQL GΝPRLQILIS RLCGSLFLHT 201 VRISYCLDGF HRLHIFΝRFF TVLLLCLFAH IVSLKTΝWKS KSSYYPRKIR 251 TFSRΝFKQRQ RISΝSFSΝPL PKK* m244-l/a244-l 96.8% identity in 274 aa overlap 10 20 30 40 50 60 m244-1. pep MPSEARQAGSDGIAALLRSVYTQΝALQEIΝQIIPQTPΞGFLLRHRΝHSRAQHAVGQRITL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I lllll a244-l MPSEARQAGSDGIAALLRΞVYTQΝALQEIΝQIIPQTPSGFLLCHRΝHSRAQHAVGQRITL 10 20 30 40 50 60 70 80 90 100 110 120 m244-l.pep LHHTHHGIRLLFACHRLHRLMDIRIELIARFRVDFLDLRΞIKCFLQLVQSHLHAHFQRIE ll llll :| I I I I I I I I I I I I I I I I I I I I hi I I I I I I I I I I I I I I I I I I I I I I I I I I a244-l LHHAHHGIGFLFACHRLHRLMDIRIELIARFRIDFLDLRSIKCFLQLVQSHLHAHFQRIE 70 80 90 100 110 120 130 140 150 160 170 179 m244-l.pep IAALIQKRHFQIILDRQHFHGKLLSGELVRIRΝFLLVAAAQVLLVCQSA-LLVFQLRFQL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I a244-l IAALIQKRHFQIILDRQHFHGKLLSGELVRIRΝFLLVAAAQVLLVCQSAQLLVFQLRFQL 130 140 150 160 170 180 180 190 200 210 220 230 239 m24 -1.pep GΝPRLQILISRLCGSLFLHTVRISYCFDGFHRLHIFNRFFTVLLLCLFAHIVSLKTNWKS I I I I I I I I I I I I I I II I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a244-1 GNPRLQILISRLCGSLFLHTVRISYCLDGFHRLHIFNRFFTVLLLCLFAHIVSLKTNWKS 190 200 210 220 230 240 240 250 260 270 m244-1. pep KSSYYPRKIRTFSRNFXQXQRIΞNSFSNPLPKKX I I I I I I I I I I I I I I I I I lllllllllllllll a244-l KSSYYPRKIRTFSRNFKQRQRISNΞFSNPLPKKX 250 260 270 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 941>: g246. seq 1 atgtacgggc ggaacggtag tactcaagcg gccgttgcct tcgttttcga 51 ccagacacag cgtgcccgtt tcggcaacgg cgaagtttac gccgctcaag 101 ccgacatcgg cagtgctgta aatatcgcgc agggctttgc gggcgaatcc 151 ggtcagttgg tccacgtcgt ctgtaagcgg tgtgccgagg ttttggtgga 201 acagttcgct gacctgttct ttggttttat ggattgcggg catcacgata 251 tgggtcggtt tttcgcctgc catttggacg ataaactcgc ccaagtcgct 301 ttccaccgcc ttaatgcctt ttgcttcaag ataatggttc agctcgattt 351 cttcgctgac catggatttg cctttgacca tcagcttgcc gtttttggct 401 gtgatgatgt cgtggataat ttggcaggct tcggcagggg tttccgccca 451 gtgtactttc acgcccaact tagtcaggtt ttcttccaac tgctccagca 501 gcgcgggtaa This corresponds to the amino acid sequence <SEQ ID 942; ORF 246.ng>: g246.pep 1 MYGRΝGSTQA AVAFVFDQTQ RARFGΝGEVY AAQADIGSAV ΝIAQGFAGES 51 GQLVHWCKR CAEVLVEQFA DLFFGFMDCG HHDMGRFFAC HLDDKLAQVA 101 FHRLΝAFCFK IMVQLDFFAD HGFAFDHQLA VFGCDDWDΝ LAGFGRGFRP 151 VYFHAQLSQV FFQLLQQRG* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 943>: m246.seq (partial) 1 ATGCACGGGC GGTACGGTGG TACTCAAGCG ACCGTTgCTT CGTTTTCCAC 51 CAGACACAGC GTACCTGTTT CAGCAACGGC AAAGTTTACG CCACTCAAAC 101 CGACATCGGC AGTGCTGTAA ATATCGCGCA GTGCTTTACG GGCGAAGCCG 151 GTCAGTTGGT CTACATCGTC TGTCAGCGGC GTACCGAGGT TTTGGTGGAA 201 CAGTTCGCTA ACCTGTTCTT TGGTTTTGTG GATAGCAGGC ATCACGATAT 251 GGGTCGGTTT TTCGCCTGCC ATTTGGACGA TGAACTCGCC CAAGTCGCTT 301 TCTACCGCTT TAATGCyTTT TGCTTCAAGA TAATGrTTCA GCTCGATTTC 351 CTCGCTGACC ATCGATTTGC CTTTGACCAT CAGCTTGCCG TTTTTGGCTG 401 TGATGATGTC GTGGATAATT TGGCAGGCTT CGGTCGGGGT TTCTGCCCG... This corresponds to the amino acid sequence <SEQ ID 944; ORF 246>: m246.pep (partial) 1 MHGRYGGTQA TVAFVFHQTQ RTCFSΝGKVY ATQTDIGSAV ΝIAQCFTGEA 51 GQLVYIVCQR RTEVLVEQFA ΝLFFGFVDSR HHDMGRFFAC HLDDELAQVA 101 FYRFΝAFCFK IMXQLDFLAD HRFAFDHQLA VFGCDDWDΝ LAGFGRGFCP ... Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 246 shows 80.0% identity over a 150 aa overlap with a predicted ORF (ORF 246.ng) from N gonorrhoeae: m246/g246 10 20 30 40 50 60 m246. pep MHGRYGGTQATVAFVFHQTQRTCFSΝGKVYATQTDIGSAVΝIAQCFTGEAGQLVYIVCQR g246 MYGRΝGSTQAAVAFVFDQTQRARFGΝGEVYAAQADIGSAVΝIAQGFAGESGQLVHWCKR 10 20 30 40 50 60 70 80 90 100 110 120 m246. pep RTEVLVEQFAΝLFFGFVDSRHHDMGRFFACHLDDELAQVAFYRFΝAFCFKIMXQLDFLAD M l l l l l l h l l l l h l M M M M M I M h l M M h h l l M M M l l l h l l g246 CAEVLVEQFADLFFGFMDCGHHDMGRFFACHLDDKLAQVAFHRLΝAFCFKIMVQLDFFAD 70 80 90 100 110 120 130 140 150 m246. pep HRFAFDHQLAVFGCDDWDNLAGFGRGFCP I MMMMMMMMMMMMM I g246 HGFAFDHQLAVFGCDDWDNLAGFGRGFRPVYFHAQLSQVFFQLLQQRGX 130 140 150 160 The following partial DNA sequence was identified in N meningitidis <SEQ ID 945>: a246.seq (partial) 1 ATGCACGGGC GGAACGGTGG TACTCAAGCG ACCGTTGCCT TCGTTTTCCA 51 CCAGACACAG CGTACCTGTT TCAGCAACGG CGAAGTTCAC GCCACTCAAA 101 CCGACATCGG CAGTGCTGTA AATATCGCGC AGTGCTTTAC GGGCGAAGCC 151 GGTCAGTTGG TCTACGTCGT CCGTTAACGG TGTGCCGAGG TTTTGGTGGA 201 ACAGTTCGCT AACCTGTTCT TTGGTTTTAT GGATTGCGGG CATCACGATA 251 TGGGTCGGTT TTTCACCTGC CATTTGGACG ATGAACTCGC CCAAGTCGCT 301 TTCCACCGCT TTAATGCCTT TTGCTTCAAG ATAATGGTTC AGCTCGATTT 351 CCTCGCTGAC CATCGATTTG CCTTTGACCA TCAGCTTGCC GTTTTTGGCT 401 GTGATGATGT CGTGGATGAT TTCGCAGGCT TCGGCCGGTG TTTCCGCCCA 451 GTGTACTTTT ACGCCCAACT TGGTCAGGTT TTCTTCCAGC TGCTCCAGCA 501 G This corresponds to the amino acid sequence <SEQ ID 946; ORF 246. a>: a246.pep (partial) 1 MHGRΝGGTQA TVAFVFHQTQ RTCFSΝGEVH ATQTDIGSAV ΝIAQCFTGEA 51 GQLVYWR*R CAEVLVEQFA ΝLFFGFMDCG HHDMGRFFTC HLDDELAQVA 101 FHRFΝAFCFK IMVQLDFLAD HRFAFDHQLA VFGCDDWDD FAGFGRCFRP 151 VYFYAQLGQV FFQLLQQ m246/a246 88.0% identity in 150 aa overlap 10 20 30 40 50 60 m246. pep MHGRYGGTQATVAFVFHQTQRTCFSΝGKVYATQTDIGSAVΝIAQCFTGEAGQLVYIVCQR I I I I I I II I I I I I I I I I I I I I I I I I I : I : I I I I I I I I I I I I I I I I I I I I I I I I I : I I a246 MHGRΝGGTQATVAFVFHQTQRTCFSΝGEVHATQTDIGSAVΝIAQCFTGEAGQLVYVVRXR 10 20 30 40 50 60 70 80 90 100 110 120 m246. pep RTEVLVEQFAΝLFFGFVDSRHHDMGRFFACHLDDELAQVAFYRFΝAFCFKIMXQLDFLAD : I I I I I I I I I I I I I I : I I I I I I I I I : I I I I I I I I II I I : I I I I I I I I I I lllllll a24 6 CAEVLVEQFAΝLFFGFMDCGHHDMGRFFTCHLDDELAQVAFHRFΝAFCFKIMVQLDFLAD 70 80 90 100 110 120 130 140 150 m24 6 . pep HRFAFDHQLAVFGCDDWDNLAGFGRGFCP I I I I I I I I I I I I I I I I I I I : : I I I I I I I a24 6 HRFAFDHQLAVFGCDDWDDFAGFGRCFRPVYFYAQLGQVFFQLLQQ 130 140 150 160 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 947>: g247. seq 1 atgaaacgta aaatgctaaa cgtaccaaag ggcggttatg atggtatgaa 51 gggttttacc attgttgaat ttctggttgc gggcctgctc agtataattg 101 tcctgatagc ggtcgtatcg agttacttta catcccggaa attaaatgat 151 gtggcaaacg agcgtcttgc cattcaacag gatttgcgga atgcggcaac 201 attaattgtc cgcgatgcaa gaatggcggg gagcttcggt tgtttcaata 251 tgtccgagca tactaaagac gatattgttg attcaagtaa tcaaactcaa 301 tctaaccttg caaaacccgg tgccaaacaa gaaaatcccc ttttttcctt 351 aaaaaggagc ggcatggata aacaactgat tcccgttgct gaatccatag 401 atattaaata tccgggtttt atccagcgcc ttaacgcatt ggttttccaa 451 tacggtatcg atgatcttga tgcgagtgct gagactgttg tagtcagcag 501 ctgttccaaa atagcaaaac cgggtaagaa aatatctacc ttgcaagaag 551 caaagagtgc attacagatt actaatgatg ataaacaaaa tggaaatatc 601 acccgtcaga aacatgtggt caatgcctat gcggtcggca ggtttggcaa 651 taatgaggaa agtttgttcc gcttccaatt ggatgataag ggcaagtggg 701 gtaatcctca gttgctcgtg aaaaaggtta aacgtatgga tgtgcggtat 751 atttatgttt ccggttgtcc tgaagatgaa gatgccggca aagaggaaaa 801 attcagatat acgaataaat tcgacaaatc caaaaatgct gttacgcctg 851 ccggggtgga ggttttattg gatagcggcc ttaatgccaa gattgccgct 901 tcttcagaca atagtattta tgcttaccgt atcaatgcga caatacgcgg 951 gggaaatgta tgcgcaaaca gaacactttg a This corresponds to the amino acid sequence <SEQ ID 948; ORF 247.ng>: g247.pep 1 MKRKMLNVPK GGY GMKGFT IVEFLVAGLL SIIVLIAWS SYFTSRKLND 51 VANERLAIQQ DLRNAATLIV RDARMAGSFG CFNMSEHTKD DIVDSSNQTQ 101 SNLAKPGAKQ ENPLFSLKRS GMDKQLIPVA ESIDIKYPGF IQRLNALVFQ 151 YGIDDLDASA ETVWSSCSK IAKPGKKIST LQEAKSALQI TNDDKQNGNI 201 TRQKHWNAY AVGRFGNNEE SLFRFQLDDK GKWGNPQLLV KKVKRMDVRY 251 IYVSGCPEDE DAGKEEKFRY TNKFDKSKNA VTPAGVEVLL DSGLNAKIAA 301 SSDNSIYAYR INATIRGGNV CANRTL* The following partial DNA sequence was identified in N meningitidis <SEQ ID 949>: m247.seq (partial) 1 ATsAGACGTA AAATGCTAAA CGTwsyArAA GGCAGTTATG ATGGTATGAA 51 AGGTTTTACC ATTATTGAAT TTTTGGTTGC GGGCCTGCTC AGTATGATTG 101 TCCTGATGGC GGTCGGATCG AGTTACTTCA CATCCCGGAA ATTAAATGAT 151 GCGGCAAACG AGCGTCTTGC CGCGCAACAG GATTTGCGGA ATGCGGCAAC 201 ATTGATTGTC CGCGATGCGA GAATGGCAGG CGGCTTCGGT TGTTTCAATA 251 TGTCCGAGCA TCCTGCAACT GATGTTATTC CCGATACGAC GCAACAAAAT 301 TCTCCTTTTT CCTTAAAAAG GAACGGTATA GATAAACTTA TTCCCATAGC 351 GGAATCTTCA AATATCAATT ATCAGAATTT TTTCCAGGTT GGTAGCGCAT 401 TGATTTTTCA ATACGGAATC GATGATGTTA ATGCAAGCAC CGCGACTACC 451 GTCGTCAGCA GCTGTGCCGC AATATCGAAA CCGGGCAAGC AAATCCCTAC 501 TTTAGAAGAT GCAAAAAAAG AATTGAAGAT TCCGGATCAG GATAAGGAGC 551 AAAATGGCAA TATAGCGCGT CAAAGGCATG TGGTCAATGC CTATGCGGTC 601 GGCAGGATTG CCGATGAGGA AAGTTTGTTC CGCTTCCAAT TGGATGATAA 651 GGGCAAGTGG GGTAATCCTC AGTTGC... This corresponds to the amino acid sequence <SEQ ID 950; ORF 247>: m247.pep (partial) 1 XRRKMLΝVXX GSYDGMKGFT IIEFLVAGLL SMIVLMAVGS SYFTSRKLND 51 AANERLAAQQ DLRNAATLIV RDARMAGGFG CFNMSEHPAT DVIPDTTQQN 101 SPFSLKRNGI DKLIPIAESS NINYQNFFQV GSALIFQYGI DDVNASTATT 151 WSSCAAISK PGKQIPTLED AKKELKIPDQ DKEQNGNIAR QRHWNAYAV 201 GRIADEESLF RFQLDDKGKW GNPQL.... Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 247 shows 69.3% identity over a 238 aa overlap with a predicted ORF (ORF 247.ng) from N gonorrhoeae: m247/g247 10 20 30 40 50 60 m247.pep XRRKMLΝVXXGSYDGMKGFTIIEFLVAGLLSMIVLMAVGSSYFTSRKLΝDAAΝERLAAQQ Mlllll hllllllllhllllllllhllhll MMMMMhMMM II g247 MKRKMLΝVPKGGYDGMKGFTIVEFLVAGLLSIIVLIAWSSYFTSRKLΝDVAΝERLAIQQ 10 20 30 40 50 60 70 80 90 100 m247. pep DLRΝAATLIVRDARMAGGFGCFΝMSEHPATDVI PDTTQQΝSPFSLKRΝ IIMMIIIIIIIIIIhlllllllM h = I = hi llllh g247 DLRΝAATLIVRDARMAGSFGCFΝMSEHTKDDIVDSSΝQTQSΝLAKPGAKQEΝPLFSLKRS 70 80 90 100 110 120 110 120 130 140 150 160 m247.pep GIDK-LIPIAESSΝIΝYQΝFFQVGSALIFQYGIDDVΝASTATTWSSCAAISKPGKQIPT g247 GMDKQLIPVAESIDIKYPGFIQRLNALVFQYGIDDLDASAETVWSSCSKIAKPGKKIST 130 140 150 160 170 180 170 180 190 200 210 220 m247.pep LEDAKKELKIPDQDKEQNGNIARQRHWNAYAVGRIAD-EESLFRFQLDDKGKWGNPQL \ -- -- W -- h i : I M l l h l h l M M M M h : : M M M M M M M M M M g247 LQEAKSALQITNDDK-QNGNITRQKHWNAYAVGRFGNNEESLFRFQLDDKGKWGNPQLL 190 200 210 220 230 g247 VKKVKRMDVRYIYVSGCPEDEDAGKEEKFRYTNKFDKSKNAVTPAGVEVLLDSGLNAKIA 240 250 260 270 280 290 The following partial DNA sequence was identified in N meningitidis <SEQ ED 951 >: a247. seq 1 ATGAGACGTA AAATGCTAAA CGTACCAAAA GGCAATTATG ATGGTATGAA 51 GGGTTTTACC ATTATTGAAT TTTTGGTTGC GGGCATGCTC AGTATGATTG 101 TCCTGATGGC GGTCGGATCG AGTTACTTCA CATCCCGGAA ATTAAATGAT 151 GCGGCAAACG AGCGTCTTTC CGCGCAACAG GATTTGCGGA ATGCGGCAAC 201 ATTGATTGTC CGCGATGCAA GAATGGCAGG GGGCTTCGGT TGTTTCAATA 251 TGTCCGAGCA TACTAAAAAT GATATTATTG TTGATCCAAG TAAGCAAACT 301 CAACATGTCC CTGTAAAACC CGGTGCCAAA CAAGAAAATC CCCTTTTTTC 351 TTTAGAGTGG GCTAATACTA ATAATACTAA TAATAATACA GCTAAATTGA 401 TTCCTATTGC TGAATCCACA GATATTAAAT ATCCGGGTTT TGCCCAGGCT 451 CGTCCGGCAT TGATTTTCCA ATACGGCATC GATGATCTTG ATGCGAGTGC 501 TGAGACTGTT GTAGTCAGCA GCTGTTCCAA AATAGCAAAA CCGGGTAAGA 551 AAATATCTAC CTTGCAAGAA GCAAAGAGTG CATTACAGAT TACTAATGAT 601 GATAAACAAA ATGGAAATAT CACCCGTCAA AGGCATGTGG TCAATGCCTA 651 TGCGGTCGGC AGGATTGCCG GTGAGGAAGG TTTGTTCCGC TTCCAATTGG 701 ATGATAAGGG CAAGTGGGGT AATCCTCAGT TGCTCGTGAA AAAGATTAGA 751 CATATGAAAG TGCGGTATAT CTATGTTTCC GACTGTCCTG AAGATGACGA 801 TGCCGGCAAA GAGGAAAAAT TCAAATATAC GGGTACATTC GACAGCTCCA 851 CAAATGCTGT TACGCCCGCC GGGGTGGAGG TTTTATTGAG TAΝCGGTACT 901 GATACCAAGA TTGCCGCTTC TTCAGACAAT CATATTTATG CTTACCGTAT 951 CGATGCGACA ATACGCGGGG GAAATGTATG CGCAAACAGA ACACTTTGA This corresponds to the amino acid sequence <SEQ ID 952; ORF 247. a>: a24 .pep 1 MRRKMLΝVPK GΝYDGMKGFT IIEFLVAGML SMIVLMAVGS SYFTSRKLND 51 AANERLSAQQ DLRNAATLIV RDARMAGGFG CFNMSEHTKN DIIVDPΞKQT 101 QHVPVKPGAK QENPLFSLEW ANTNNTNNNT AKLIPIAEST DIKYPGFAQA 151 RPALIFQYGI DDLDASAETV WSSCSKIAK PGKKISTLQE AKSALQITND 201 DKQNGNITRQ RHWNAYAVG RIAGEEGLFR FQLDDKGKWG NPQLLVKKIR 251 HMKVRYIYVS DCPEDDDAGK EEKFKYTGTF DSSTNAVTPA GVEVLLSXGT 301 DTKIAASSDN HIYAYRIDAT IRGGNVCANR TL* m247/a247 70.9% identity in 244 aa overlap 10 20 30 40 50 60 m247. pep XRRKMLNVXXGSYDGMKGFTIIEFLVAGLLSMIVLMAVGSSYFTSRKLNDAANERLAAQQ lllllll I : I I I I I I I I I I I I I I I I : I I I I I I I I I I I I II I I II I I I I I I I I I : I I I a247 MRRKMLNVPKGNYDGMKGFTIIEFLVAGMLSMIVLMAVGSSYFTSRKLNDAANERLSAQQ 10 20 30 40 50 60 70 80 90 100 m247.pep DLRNAATLIVRDARMAGGFGCFNMSEHPATDVI PDTTQQNSPFSLK- I I I I I I I I I II I I I I I I I I I I I I I I I I : I : I I : I : I III: a247 DLRNAATLIVRDARMAGGFGCFNMSEHTKNDIIVDPSKQTQHVPVKPGAKQENPLFSLEW 70 80 90 100 110 120 110 120 130 140 150 160 m247.pep RNGIDKLIPIAESSNINYQNFFQVGSALIFQYGIDDVNASTATTWSSCAAISK I : I I I I I I I I :: I : I : I I: I I I I I I I I I I :: I I : |: I I I I |: I : I a247 ANTNNTNNNTAKLIPIAESTDIKYPGFAQARPALIFQYGIDDLDASAETVWSSCSKIAK 130 140 150 160 170 180 170 180 190 200 210 220 m247.pep PGKQIPTLEDAKKELKIPDQDKEQNGNIARQRHWNAYAVGRIADEESLFRFQLDDKGKW 111:1 I I :: I I : I : I :: I I I I I I I : I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I a247 PGKKISTLQEAKSALQITNDDK-QNGNITRQRHWNAYAVGRIAGEEGLFRFQLDDKGKW 190 200 210 220 230 m247 . pep GNPQL I I I I I a2 7 GNPQLLVKKIRHMKVRYIYVSDCPEDDDAGKEEKFKYTGTFDSSTNAVTPAGVEVLLSXG 240 250 260 270 280 290 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 953>: g247-l.seq (partial) 1 CCCGGTGCCA AACAAGAAAA TCCCCTTTTT TCCTTAAAAA GGAGCGGCAT 51 GGATAAACAA CTGATTCCCG TTGCTGAATC CATAGATATT AAATATCCGG 101 GTTTTATCCA GCGCCTTAAC GCATTGGTTT TCCAATACGG TATCGATGAT 151 CTTGATGCGA GTGCTGAGAC TGTTGTAGTC AGCAGCTGTT CCAAAATAGC 201 AAAACCGGGT AAGAAAATAT CTACCTTGCA AGAAGCAAAG AGTGCAT C 251 AGATTACTAA TGATGATAAA CAAAATGGAA ATATCACCCG TCAGAAACAT 301 GTGGTCAATG CCTATGCGGT CGGCAGGTTT GGCAATAATG AGGAAAGTTT 351 GTTCCGCTTC CAATTGGATG ATAAGGGCAA GTGGGGTAAT CCTCAGTTGC 401 TCGTGAAAAA GGTTAAACGT ATGGATGTGC GGTATATTTA TGTTTCCGGT 451 TGTCCTGAAG ATGAAGATGC CGGCAAAGAG GAAAAATTCA GATATACGAA 501 TAAATTCGAC AAATCCAAAA ATGCTGTTAC GCCTGCCGGG GTGGAGGTTT 551 TATTGGATAG CGGCCTTAAT GCCAAGATTG CCGCTTCTTC AGACAATAGT 601 ATTTATGCTT ACCGTATCAA TGCGACAATA CGCGGGGGAA ATGTATGCGC 651 AAACAGAACA CTTTGA This corresponds to the amino acid sequence <SEQ ID 954; ORF 247-1. ng>: g247-l.pep (partial) 1 PGAKQEΝPLF ΞLKRSGMDKQ LIPVAESIDI KYPGFIQRLΝ ALVFQYGIDD 51 LDASAETVW SSCSKIAKPG KKISTLQEAK ΞALQITΝDDK QΝGΝITRQKH 101 VVΝAYAVGRF GΝΝEESLFRF QLDDKGKWGΝ PQLLVKKVKR MDVRYIYVSG 151 CPEDEDAGKE EKFRYTΝKFD KΞKΝAVTPAG VEVLLDSGLΝ AKIAAΞSDΝS 201 IYAYRIΝATI RGGΝVCAΝRT L* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 955>: 247-l.seq 1 ATGAGACGTA AAATGCTAAA CGTACCAAAA GGCAGTTATG ATGGTATGAA 51 AGGTTTTACC ATTATTGAAT TTTTGGTTGC GGGCCTGCTC AGTATGATTG 101 TCCTGATGGC GGTCGGATCG AGTTACTTCA CATCCCGGAA ATTAAATGAT 151 GCGGCAAACG AGCGTCTTGC CGCGCAACAG GATTTGCGGA ATGCGGCAAC 201 ATTGATTGTC CGCGATGCGA GAATGGCAGG CGGCTTCGGT TGTTTCAATA 251 TGTCCGAGCA TCCTGCAACT GATGTTATTC CCGATACGAC GCAACAAAAT 301 TCTCCTTTTT CCTTAAAAAG GAACGGTATA GATAAACTTA TTCCCATAGC 351 GGAATCTTCA AATATCAATT ATCAGAATTT TTTCCAGGTT GGTAGCGCAT 401 TGATTTTTCA ATACGGAATC GATGATGTTA ATGCAAGCAC CGCGACTACC 451 GTCGTCAGCA GCTGTGCCGC AATATCGAAA CCGGGCAAGC AAATCCCTAC 501 TTTAGAAGAT GCAAAAAAAG AATTGAAGAT TCCGGATCAG GATAAGGAGC 551 AAAATGGCAA TATAGCGCGT CAAAGGCATG TGGTCAATGC CTATGCGGTC 601 GGCAGGATTG CCGATGAGGA AGGTTTGTTC CGCTTCCAAT TGGATGATAA 651 GGGCAAGTGG GGTAATCCTC AGTTGCTCGT GAAAAAGGTT AGACATATGA 701 AAGTGCGGTA TATCTATGTT TCCGGCTGTC CTGAAGATGA CGATGCCGGC 751 AAAGAGGAAA CATTCAAATA TACGGATAAA TTCGACAGCG CCCAAAATGC 801 TGTTACGCCC GCCGGGGTGG AGGTTTTATT GAGTAGCGGT ACTGATACCA 851 AGATTGCCGC TTCTTCAGAC AATCATATTT ATGCTTACCG TATCGATGCG 901 ACAATACGCG GGGGAAATGT ATGCGCAAAC AGAACACTTT GA This corresponds to the amino acid sequence <SEQ ED 956; ORF 247-l>: m247-l.pep 1 MRRKMLΝVPK GSYDGMKGFT IIEFLVAGLL SMIVLMAVGS SYFTSRKLND 51 AANERLAAQQ DLRNAATLIV RDARMAGGFG CFNMSEHPAT DVIPDTTQQN 101 SPFΞLKRNGI DKLIPIAESS NINYQNFFQV GΞALIFQYGI DDVNASTATT 151 VVSSCAAISK PGKQIPTLED AKKELKIPDQ DKEQNGNIAR QRHVVNAYAV 201 GRIADEEGLF RFQLDDKGKW GNPQLLVKKV RHMKVRYIYV SGCPEDDDAG 251 KEETFKYTDK FDΞAQNAVTP AGVEVLLSSG TDTKIAASSD NHIYAYRIDA 301 TIRGGNVCAN RTL* m247-l / g247-l 72.1% identity in 222 aa overlap 70 80 90 100 110 120 m247-1.pep NAATLIVRDARMAGGFGCFNMSEHPATDVIPDTTQQNSPFSLKRNGIDK-LIPIAESSNI I : I :| I I I I I :l : I I I I I : I I I : I g247-l PGAKQENPLFSLKRSGMDKQLIPVAEΞIDI 10 20 30 130 140 150 160 170 180 m247 - 1 . pep NYQNFFQVGΞALIFQYGI DDVNASTATTVVSSCAAI SKPGKQIPTLEDAKKELKI PDQDK : I : I : I : I I : I I I I I I I : : I I : I : | I I I I : I : | I I I : | I I : : I I : I : I : : | I g247-l KYPGFIQRLNALVFQYGIDDLDASAETVWSSCSKIAKPGKKISTLQEAKSALQITNDDK 40 50 60 70 80 90 190 200 210 220 230 240 m247-l.pep EQNGNIARQRHWNAYAVGRIAD-EEGLFRFQLDDKGKWGNPQLLVKKVRHMKVRYIYVS I I I I I : I I : I I I I I I I I I I ::: I I : I I I I I I I I I I I I I I I I I I I I I I :: I g247-l -QNGNITRQKHWNAYAVGRFGNNEESLFRFQLDDKGKWGNPQLLVKKVKRMDVRYIYVS 100 110 120 130 140 250 260 270 280 290 300 m247-l.pep GCPEDDDAGKEETFKYTDKFDSAQNAVTPAGVEVLLΞΞGTDTKIAAΞΞDNHIYAYRIDAT 11111:111111 I : I I : I I I ::: I I I I I I I I I I I I : I I :: I I I I I I I I I I I I I I :| I g247-l GCPEDEDAGKEEKFRYTNKFDKSKNAVTPAGVEVLLDΞGLNAKIAASSDNSIYAYRINAT 150 160 170 180 190 200 310 m247- -1.pep IRGGNVCANRTLX 1111111111111 g247- -1 IRGGNVCANRTLX 210 220 The following partial DNA sequence was :identified in N menin a247 -l.seq (partial) 1 AATAATACAG CTAAATTGAT TCCTATTGCT GAATCCACAG ATATTAAATA 51 TCCGGGTTTT GCCCAGGCTC GTCCGGCATT GATTTTCCAA TACGGCATCG 101 ATGATCTTGA TGCGAGTGCT GAGACTGTTG TAGTCAGCAG CTGTTCCAAA 151 ATAGCAAAAC CGGGTAAGAA AATATCTACC TTGCAAGAAG CAAAGAGTGC 201 ATTACAGATT ACTAATGATG ATAAACAAAA TGGAAATATC ACCCGTCAAA 251 GGCATGTGGT CAATGCCTAT GCGGTCGGCA GGATTGCCGG TGAGGAAGGT 301 TTGTTCCGCT TCCAATTGGA TGATAAGGGC AAGTGGGGTA ATCCTCAGTT 351 GCTCGTGAAA AAGATTAGAC ATATGAAAGT GCGGTATATC TATGTTTCCG 401 ACTGTCCTGA AGATGACGAT GCCGGCAAAG AGGAAAAATT CAAATATACG 451 GGTACATTCG ACAGCTCCAC AAATGCTGTT ACGCCCGCCG GGGTGGAGGT 501 TTTATTGAGT AGCGGTACTG ATACCAAGAT TGCCGCTTCT TCAGACAATC 551 ATATTTATGC TTACCGTATC GATGCGACAA TACGCGGGGG AAATGTATGC 601 GCAAACAGAA CACTTTGA This corresponds to the amino acid sequence <SEQ ID 958; ORF 247-1. a>: a247-l.pep (partial) .. 1 ΝΝTAKLIPIA EΞTDIKYPGF AQARPALIFQ YGIDDLDASA ETVWSSCSK 51 IAKPGKKIΞT LQEAKSALQI TΝDDKQΝGΝI TRQRHWΝAY AVGRIAGEEG 101 LFRFQLDDKG KWGΝPQLLVK KIRHMKVRYI YVSDCPEDDD AGKEEKFKYT 151 GTFDSSTΝAV TPAGVEVLLS SGTDTKIAAS SDΝHIYAYRI DATIRGGΝVC 201 AΝRTL* m247-l / a247-l 80.6% identity in 206 aa overlap 10 20 30 a247-1. pep ΝΝTAKLIPIAESTDIKYPGFAQARPALIFQ I : I I I I I I I I :: I : I : I I : I I I I I m247-l GFGCFΝMSEHPATDVIPDTTQQΝSPFSLKRΝGIDKLIPIAESSΝIΝYQΝFFQVGSALIFQ 80 90 100 110 120 130 40 50 60 70 80 89 a247-l.pep YGIDDLDASAETVWΞSCSKIAKPGKKISTLQEAKΞALQITΝDDK-QΝGΝITRQRHVVΝA I I I I I :: I I: I : I I I I I : I : I I I I : I I I ::| I : hi :: I I I I I I I : I I I I I I I I m247-l YGIDDVΝASTATTVVSSCAAISKPGKQIPTLEDAKKELKIPDQDKEQΝGΝIARQRHVVΝA 140 150 160 170 180 190 90 100 110 120 130 140 149 a247-1. ep YAVGRIAGEEGLFRFQLDDKGKWGΝPQLLVKKIRHMKVRYIYVSDCPEDDDAGKEEKFKY lllllll I I I I I I I I I I I II I II I I I I I II 1:1 I I I I I I I I I I I I I I I I I I I I I III m247-1 YAVGRIADEEGLFRFQLDDKGKWGNPQLLVKKVRHMKVRYIYVΞGCPEDDDAGKEETFKY 200 210 220 230 240 250 150 160 170 180 190 200 a247-1. pep TGTFDSSTNAVTPAGVEVLLSΞGTDTKIAASSDNHIYAYRIDATIRGGNVCANRTLX I ll I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m247-l TDKFDΞAQNAVTPAGVEVLLSSGTDTKIAAΞSDNHIYAYRIDATIRGGNVCANRTLX 260 270 280 290 300 310 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 959>: g248.seq 1 atgcgcaaac agaacacttt gacaggaatc ccgacttctg acggacagag 51 ggggtccgca ctgtttatcg tgctgatggt gatgatagtc gtggcctttt 101 tggttgtaac tgccgcccag tcctacaata ccgaacagag gatcagtgcc 151 aacgaatcag acaggaaatt ggctttgtct ttagccgagg cggctttgcg 201 ggagggcgaa tttcaggttt tggatttgga atatgctgcg gacagtaagg 251 ttacgtttag cgaaaactgt gaaaaaggtc tgtgtaccgc agtgaatgtg 301 cggacaaata ataatggtag tgaagaggct tttggcaata tcgtggtgca 351 aggcaagccc gccgttgagg cggtgaaacg ttcttgccct gcaaagtctg 401 gcaaaaattc taccgacctg tgcattgaca ataaagggat ggaatataat 451 aaaggcgcgg caggcgtcag caaaatgccg cgctatatta tcgaatattt 501 aggcgtgaag aacggacaaa atgtttatcg ggttactgcc aaggcttggg 551 gtaagaatgc caataccgtg gtcgtccttc aatcttatgt aggcaataat 601 gatgagcaat aa This corresponds to the amino acid sequence <SEQ ID 960; ORF 248.ng>: g248.pep 1 MRKQΝTLTGI PTSDGQRGSA LFIVLMVMIV VAFLWTAAQ SYΝTEQRISA 51 ΝESDRKLALS LAEAALREGE FQVLDLEYAA DSKVTFSEΝC EKGLCTAVΝV 101 RTΝΝΝGSEEA FGΝIWQGKP AVEAVKRSCP AKSGKΝSTDL CIDΝKGMEYΝ 151 KGAAGVSKMP RYIIEYLGVK ΝGQΝVYRVTA KAWGKΝAΝTV WLQSYVGΝΝ 201 DEQ* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 961>: m248.seq (partial) 1 .. GGGTTTGCAC TGTTAATCGT GCTGATGGTG ATrATCGTCG TGGCT . TywT 51 gG TGTAACT GCCGCGCAGT CTTACAATAC cGAGCAGCGk ATCAGTkCCA 101 ACGAATCAGA CAGGAAATTG GCTwTGTCTT TGGCCGAGkC GkCTwTGCGG 151 GAAGGCGAAC TTCAGGTTTT GGATTTGGAA TATGATACGG ACAGTAAGGT 201 TACATTTAGC GAAAACTGTG GAAAAGGTCT GTsTGCCGCA GTGAATGTGC 251 GGACAAATAA TGATAATGAA GAGGCTTTTG ACAATATCGT GGTGCAAGGC 301 AAGCCCACCG TTGAGGCGGT GAAGCGTTCT TGCCCTGCAA ATTCTACCGA 351 CCTGTGCATT GACAAGAAAG GG TGGAATA TAAGAAAGGC ACGAGAAGCG 401 TCAC.AAAAT GCCACGTTAT ATTATCGAAT ATTTGGGCGT GwAGAACGGA 451 GAAAATGTTT ATCGGGTTAC TGCCAAGGCT TGGGGtAAGA ATGCCAATAC 501 CGTGGTCGTC CTTCAATCTT ATGTAAGCAA TAATGATGAG TAA This corresponds to the amino acid sequence <SEQ ID 962; ORF 248>: m248.pep (partial) 1 .. GFALLIVLMV XIWAFXXVT AAQSYΝTEQR ISXΝESDRKL AXSLAEXXXR 51 EGELQVLDLE YDTDSKVTFS EΝCGKGLXAA VΝVRTΝΝDΝE EAFDΝI QG 101 KPTVEAVKRS CPAΝSTDLCI DKKGXEYKKG TRSVTKMPRY IIEYLGVXΝG 151 EΝVYRVTAKA WGKΝAΝTVW LQSYVSΝΝDE * Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 248 shows 81.1% identity over a 185 aa overlap with a predicted ORF (ORF 248. ng) from N gonorrhoeae: m248/g248 10 20 30 40 m248.pep GFALLIVLMVXIWAFXXVTAAQSYΝTEQRISXΝESDRKLAXS g248 MRKQNTLTGIPTSDGQRGSALFIVLMVMIWAFLWTAAQSYNTEQRISANESDRKLALS 10 20 30 40 50 60 50 60 70 80 90 100 m248. pep LAEXXXREGELQVLDLEYDTDSKVTFSENCGKGLXAAVNVRTNND-NEEAFDNIWQGKP III 111 1111111 MIIMMIM III Mill IIIIIMI g248 LAEAALREGEFQVLDLEYAADSKVTFSENCEKGLCTAVNVRTNNNGSEEAFGNIWQGKP 70 80 90 100 110 120 110 120 130 140 150 m248. pep TVEAVKRSCPA NSTDLCIDKKGXEYKKGTRSVTKMPRYI IEYLGVXNGENVYRVTA MIMIMMI MMMMMI 11 = 11= MMMMIMMM Mil l g248 AVEAVKRSCPAKSGKNSTDLCIDNKGMEYNKGAAGVSKMPRYIIEYLGVKNGQNVYRVTA 130 140 150 160 170 180 160 170 180 m248. pep KAWGKNANTVWLQSYVSNNDEX g248 KAWGKNANTVWLQSYVGNNDEQX 190 200 The following partial DNA sequence was identified in N meningitidis <SEQ ID 963>: a248.seq 1 ATGCGCAAAC AGAACACTTT GACGGGAATC CCGACTTCTG ACGGACAGAG 51 GGGGTTTGCA CTGTTTATCG TGCTGATGGT GATGATCGTC GTGGCTTTTT 101 TGGTTGTAAC TGCCGCGCAG TCTTACAATA CCGAGCAGCG GATCAGTGCC 151 AACGAATCAG ACAGGAAATT GGCTTTGTCT TTGGCCGAGG CGGCTTTGCG 201 GGAAGGCGAA CTTCAGGTTT TGGATTTGGA ATATGATACG GACAGTAAGG 251 TTACATTTAG CGAAAACTGT GGAAAAGGTC TGTGTACCGC AGTGAATGTG 301 CGGACAAATA ATGATAATGA AGAGGCTTTT GACAATATCG TGGTGCAAGG 351 CAAGCCCACC GTTGAGGCGG TGAAGCGTTC TTGCACTGCA AAATCTACAG 401 GCCTGTGCAT TGACAATAAA GGGATGGAAT ATAAGAAAGG CACGCAAAGC 451 GTCAGCAAAA TGCCACGTTA TATTATCGAA TATTTGGGCG TGAAGAACGG 501 AGAAAATGTT TATCGGGTTA CTGCCAAGGC TTGGGGTAAG AATGCCAATA 551 CCGTGGTCGT CCTTCAATCT TATGTAAGCA ATAATGATGA GTAA This corresponds to the amino acid sequence <SEQ ID 964; ORF 248.a>: a248.pep 1 MRKQΝTLTGI PTSDGQRGFA LFIVLMVMIV VAFLWTAAQ SYΝTEQRISA 51 ΝESDRKLALS LAEAALREGE LQVLDLEYDT DSKVTFSEΝC GKGLCTAVΝV 101 RTΝΝDΝEEAF DΝIVVQGKPT VEAVKRSCTA KSTGLCIDΝK GMEYKKGTQS 151 VSKMPRYIIE YLGVKΝGEΝV YRVTAKAWGK ΝAΝTVWLQS YVSΝΝDE* m248/a248 89.4% identity in 180 aa overlap 10 20 30 40 m248.pep GFALLIVLMVXIWAFXXVTAAQSYΝTEQRISXΝESDRKLAXS 1111: 11111 I I I I I I I I I I I I I I I I I I I l l l l l l l l I a2 8 MRKQΝTLTGIPTSDGQRGFALFIVLMVMIWAFLWTAAQSYΝTEQRISAΝESDRKLALS 10 20 30 40 50 60 50 60 70 80 90 100 m248. pep LAEXXXREGELQVLDLEYDTDSKVTFSEΝCGKGLXAAVΝVRTΝΝDΝEEAFDΝIWQGKPT III I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I a248 LAEAALREGELQVLDLEYDTDSKVTFSEΝCGKGLCTAVΝVRTΝΝDΝEEAFDΝIWQGKPT 70 80 90 100 110 120 110 120 130 140 150 160 m248. pep VEAVKRSCPAΝSTDLCIDKKGXEYKKGTRSVTKMPRYIIEYLGVXΝGEΝVYRVTAKAWGK llllllll I : I I I I I I: I I I I I I I I: I I : I I I I I I I I I I I I lllllllllllllll a248 VEAVKRSCTAKSTGLCIDΝKGMEYKKGTQSVSKMPRYIIEYLGVKΝGEΝVYRVTAKAWGK 130 140 150 160 170 180 170 180 m248. pep ΝAΝTWVLQSYVSΝΝDEX I I I I I I I I I I I I I I I I I I a248 NANTVWLQSYVSNNDEX 190 The following partial DNA sequence was identified in N meningitidis <SEQ ID 965>: 1 ATGCGCAAAC AGAACACTTT GACGGGAATC CCGACTTCTG ACGGACAGAG 51 GGGGTTTGCA CTGTTTATCG TGCTGATGGT GATGATCGTC GTGGCTTTTT 101 TGGTTGTAAC TGCCGCGCAG TCTTACAATA CCGAGCAGCG GATCAGTGCC 151 AACGAATCAG ACAGGAAATT GGCTTTGTCT TTGGCCGAGG CGGCTTTGCG 201 GGAAGGCGAA CTTCAGGTTT TGGATTTGGA ATATGATACG GACAGTAAGG 251 TTACATTTAG CGAAAACTGT GGAAAAGGTC TGTGTGCCGC AGTGAATGTG 301 CGGACAAATA ATGATAATGA AGAGGCTTTT GACAATATCG TGGTGCAAGG 351 CAAGCCCACC GTTGAGGCGG TGAAGCGTTC TTGCCCTGCA AATTCTACCG 401 ACCTGTGCAT TGACAAGAAA GGGATGGAAT ATAAGAAAGG CACGAGAAGC 451 GTCAGCAAAA TGCCACGTTA TATTATCGAA TATTTGGGCG TGAAGAACGG 501 AGAAAATGTT TATCGGGTTA CTGCCAAGGC TTGGGGTAAG AATGCCAATA 551 CCGTGGTCGT CCTTCAATCT TATGTAAGCA ATAATGATGA GTAA This corresponds to the amino acid sequence <SEQ ID 966; ORF 248-l>: m248-l.pep 1 MRKQΝTLTGI PTSDGQRGFA LFIVLMVMIV VAFLWTAAQ SYΝTEQRISA 51 ΝESDRKLALS LAEAALREGE LQVLDLEYDT DSKVTFSEΝC GKGLCAAVΝV 101 RTΝΝDΝEEAF DΝIVVQGKPT VEAVKRSCPA ΝSTDLCIDKK GMEYKKGTRΞ 151 VSKMPRYIIE YLGVKΝGEΝV YRVTAKAWGK ΝAΝTVWLQS YVSΝΝDE* m248-l/g248 39.1% identity in 202 aa overlap 10 20 30 40 50 60 m248-1. pep MRKQΝTLTGIPTΞDGQRGFALFIVLMVMIWAFLVVTAAQSYΝTEQRISAΝESDRKLALS I I I I I I I I II I I I I I I I I I I I II I I I I I I I II I I I I I I I I I I I I I I I I I I I II I I I I I I g248 MRKQΝTLTGIPTSDGQRGSALFIVLMVMIWAFLVVTAAQSYΝTEQRIΞAΝESDRKLALS 10 20 30 40 50 60 70 80 90 100 110 119 m248-1.pep LAEAALREGELQVLDLEYDTDSKVTFSEΝCGKGLCAAVΝVRTΝΝD-ΝEEAFDΝIVVQGKP llllll lllll :| I I I I I I I I I I I I 1:1 I I I I I I I: :| I II g248 LAEAALREGEFQVLDLEYAADSKVTFSEΝCEKGLCTAVΝVRTΝΝΝGΞEEAFGΝIVVQGKP 70 80 90 100 110 120 120 130 140 150 160 170 m248-l.pep TVEAVKRSCPA ΝSTDLCIDKKGMEYKKGTRSVSKMPRYIIEYLGVKΝGEΝVYRVTA : I I I I I I I I I I I I I I I I I I : I I I I I : I I : : I I I I I I I I I I I I I I I I I : I I I I I I I g248 AVEAVKRSCPAKSGKΝΞTDLCIDΝKGMEYΝKGAAGVSKMPRYIIEYLGVKΝGQΝVYRVTA 130 140 150 160 170 180 180 190 m248-l .pep KAWGKΝAΝTVWLQΞYVSΝΝDEX g2 8 KAWGKNANTVWLQSYVGNNDEQX 190 200 m248-l/a248 97.0% identity in 197 aa overlap 10 20 30 40 50 60 m248-1.pep MRKQNTLTGIPTSDGQRGFALFIVLMVMIWAFLWTAAQSYNTEQRISANESDRKLALΞ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a248 MRKQNTLTGIPTΞDGQRGFALFIVLMVMIWAFLWTAAQSYNTEQRISANESDRKLALS 10 20 30 40 50 60 70 80 90 100 110 120 m248-1 . pep LAEAALREGELQVLDLEYDTDSKVTFSENCGKGLCAAVNVRTNNDNEEAFDNIWQGKPT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I h i I I I I I I I I I I I I I I I I I I I I I I I a248 LAEAALREGELQVLDLEYDTDSKVTFSENCGKGLCTAVNVRTNNDNEEAFDNIVVQGKPT 70 80 90 100 110 120 130 140 150 160 170 180 m248-1. pep VEAVKRSCPANSTDLCIDKKGMEYKKGTRSVSKMPRYIIEYLGVKNGENVYRVTAKAWGK llllllll I : I I I I I 1:1 I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a2 8 VEAVKRSCTAKSTGLCIDNKGMEYKKGTQSVSKMPRYIIEYLGVKNGENVYRVTAKAWGK 130 140 150 160 170 180 190 m248-1. pep NANTVVVLQSYVSNNDEX I I I I I I I I I I I I I I I I I I a248 NANTVVVLQSYVSNNDEX 190 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 967>: g249.seq 1 atgaagaata atgattgctt gcgcctgaaa aatccccagt ccggtatggc 51 gttgatagaa gtcttggtcg ctatgctcgt tctgaccatc ggtattttgg 101 cattgctgtc cgtacagttg cggacagtcg cttccgtcag ggaggcggaa 151 acgcaaacca tcgtcagcca aatcacgcaa aacctgatgg aaggaatgtt 201 gatgaatccg accattgatt tggacagcaa caagaaaaac tatagtcttt 251 acatgggaaa acagacacta tcagctgtgg atggtgagtt tatgcttgat 301 gccgagaaaa gtaaggcgca gttggcagag gaacaattga agagatttag 351 tcatgagctg aaaaatgcct tgccggatgc ggtagctatt cattacgccg 401 tctgcaagga ttcgtcgggt gacgcgccga cattgtccga cagcggtgct 451 ttttcttcaa attgcgacaa taaggcaaac ggggatactt tgattaaagt 501 attgtgggta aatgattcgg caggggattc ggatatttcc cgtacgaatc 551 ttgaagtgag cggcgacaat atcgtatata cctatcaggc aagggtcgga 601 ggtcgtgaat ga This corresponds to the amino acid sequence <SEQ ID 968; ORF 249.ng>: g249.pep 1 MKΝΝDCLRLK ΝPQSGMALIE VLVAMLVLTI GILALLSVQL RTVASVREAE 51 TQTIVSQITQ ΝLMEGMLMΝP TIDLDSΝKKΝ YSLYMGKQTL SAVDGEFMLD 101 AEKSKAQLAE EQLKRFSHEL KΝALPDAVAI HYAVCKDSSG DAPTLSDSGA 151 FSSΝCDΝKAΝ GDTLIKVLWV ΝDSAGDSDIS RTΝLEVSGDΝ IVYTYQARVG 201 GRE* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 969>: m249. seq 1 ATGAAGAATA ATGATTGCTT CCGCCTGAAA GATTCCCAGT CCGGTATGGC 51 GCTGATAGAA GTCTTGGTTG CTATGCTCGT TCTGACCATC GGTATTTTGG 101 CACTATTGTC TGTACAGTTG CGGACAGTCΝ ΝΝΝΝΝΝΝΝΝΝ ΝΝΝΝΝΝΝΝΝΝ 151 ΝΝΝΝΝΝΝΝΝΝ ΝΝΝΝΝΝΝΝΝΝ ΝΝΝΝΝΝΝΝΝΝ ΝΝΝTTGATGG AGGGAATGTT 201 GATGAATCCG ACCATTGATT CGGACAGCAA CAAGAAAAAC TATAATCTTT 251 ACATGGGAAA CCATACACTA TCAGCTGTGG ATGGCGATTT TGCGATTGAT 301 GCCATGAAAA CTAAGGGGCA ATTGGCAGAG GCACAATTGA AGAGATTTAG 351 TTATGAGCTG AAAAATGCCT TGCCGGATGC GGCAGCCATC CATTACGCCG 401 TCTGCAAGGA TTCGTCGGGT AACGCGCCGA CATTGTCCGG CAATGCTTTT 451 TCTTCAAATT GCGACAATAA GGCAAACGGG GATACTTTAA TTAAAGTATT 501 GTGGGTAAAT GATTCGGCAG GGGATTCGGA TATTTCCCGT ACGAATCTTG 551 AGGTGAGCGG CGACAATATC GTATATACTT ATCAGGCAAG GGTCGGAGGT 601 CGGGAATGA This corresponds to the amino acid sequence <SEQ LD 970; ORF 249>: m249. pep 1 MKΝΝDCFRLK DSQSGMALIE VLVAMLVLTI GILALLSVQL RTVXXXXXXX 51 XXXXXXXXXX XLMEGMLMΝP TIDSDSΝKKΝ YΝLYMGΝHTL SAVDGDFAID 101 AMKTKGQLAE AQLKRFSYEL KΝALPDAAAI HYAVCKDSSG ΝAPTLSGΝAF 151 SSΝCDΝKAΝG DTLIKVLWVΝ DSAGDSDISR TΝLEVSGDΝI VYTYQARVGG 201 RE* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 249 shows 81.3% identity over a 203 aa overlap with a predicted ORF (ORF 249.ng) from N gonorrhoeae: m249/g249 10 20 30 40 50 60 m249.pep MKΝΝDCFRLKDSQSGMALIEVLVAMLVLTIGILALLSVQLRTVXXXXXXXXXXXXXXXXX g249 MKNNDCLRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVASVREAETQTIVSQITQ 10 20 30 40 50 60 70 80 90 100 110 120 m249. pep XLMEGMLMNPTIDSDSNKKNYNLYMGNHTLSAVDGDFAIDAMKTKGQLAEAQLKRFSYEL 1 1 1 1 1 1 1 1 1 1 1 1 I I I I M h l l l h M l l l l l h l M l h h l l l l I M g249 NLMEGMLMNPTIDLDSNKKNYSLYMGKQTLSAVDGEFMLDAEKSKAQLAEEQLKRFSHEL 70 80 90 100 110 120 130 140 150 160 170 179 m249. ep KNALPDAAAIHYAVCKDSSGNAPTLSGN-AFSSNCDNKANGDTLIKVLWVNDSAGDSDIS : M M M M M M M M M M M I M M M M g249 KNALPDAVAIHYAVCKDSSGDAPTLSDSGAFSSNCDNKANGDTLIKVLWVNDSAGDSDIS 130 140 150 160 170 180 180 190 200 m249.pep RTNLEVSGDNIVYTYQARVGGREX MMMMMMMMMMMM g2 9 RTNLEVSGDNIVYTYQARVGGREX 190 200 The following partial DNA sequence was identified in N meningitidis <SEQ ID 971>: a249.seq 1 ATGAAGAATA ATGATTGCTT CCGCCTGAAA AACCCCCAGT CCGGTATGGC 51 GCTGATAGAA GTCTTGGTCG CTATGCTCGT TCTGACCATC GGTATTTTGG 101 CACTATTGTC TGTTCAGTTG CGGACAGTCG CTTCCGTCAG GGAGGCAGAG 151 ACGCAAACCA TCGTCAGTCA AATCACGCAA AACCTGATGG AAGGAATGTT 201 GATGAATCCG ACCATTGATT CGGACAGCAA CAAGAAAAAC TATAATCTTT 251 ACATGGGAAA CCATCATGCA CTATCAGTTG TGGATGGCGA TTTTCAGGTT 301 GATGCCATAA AAACTAAGAC GCAGTTGGCA GAGGCACAAT TGAAGAGATT 351 TAGTTATGAG CTGAAAAATG CCTTGCCGGA TGCGGCAGCC ATCCATTACG 401 CCGTCTGCAA GGATTCGTCG GGTGTTGCGC CGACATTGTC CGCCGGCAGT 451 ACTTTTTCTT CAAATTGCGA TGGTAGTGCA AATGGGGATA CTTTGATTAA 501 AGTATTGTGG GTAAATGATT CGGCAGGGGA TTCGGATATC GCCCGTACGA 551 ATCTTGAGAC GAACGGCAAC AATATCGTAT ATACCTATCA GGCAAGGGTC 601 GGAGGTCGGG AATGA This corresponds to the amino acid sequence <SEQ ID 972; ORF 249. a>: a249.pep 1 MKΝΝDCFRLK ΝPQSGMALIE VLVAMLVLTI GILALLSVQL RTVASVREAE 51 TQTIVSQITQ ΝLMEGMLMΝP TIDSDSΝKKΝ YΝLYMGΝHHA LSVVDGDFQV 101 DAIKTKTQLA EAQLKRFSYE LKΝALPDAAA IHYAVCKDSS GVAPTLSAGS 151 TFSSΝCDGSA ΝGDTLIKVLW VΝDSAGDSDI ARTΝLETΝGΝ ΝIVYTYQARV 201 GGRE* m249/a249 81.9% identity in 204 aa overlap 10 20 30 40 50 60 m249. pep MKΝΝDCFRLKDSQSGMALIEVLVAMLVLTIGILALLSVQLRTVXXXXXXXXXXXXXXXXX I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : : : a249 MKΝΝDCFRLKΝPQSGMALIEVLVAMLVLTIGILALLSVQLRTVASVREAETQTIVSQITQ 10 20 30 40 50 60 70 80 90 100 110 119 m249. pep XLMEGMLMΝPTIDSDSΝKKΝYΝLYMGΝH-TLSAVDGDFAIDAMKTKGQLAEAQLKRFSYE I I I I I I I I I I I I I I I I I I I I I I I I I II : I I : I I I I I : I I : I I I I I I I I I I I I I I I I a249 ΝLMEGMLMΝPTIDSDSΝKKΝYΝLYMGΝHHALSWDGDFQVDAIKTKTQLAEAQLKRFSYE 70 80 90 100 110 120 120 130 140 150 160 170 m249.pep LKΝALPDAAAIHYAVCKDSSGΝAPTLS-GΝAFSSΝCDΝKAΝGDTLIKVLWVΝDSAGDSDI I I I I I I I I I I I I I I I I I I I I I I I I I I h: I I I I I h: I I I I I I I I II I I I I I I I I I I I a249 LKΝALPDAAAIHYAVCKDSSGVAPTLSAGSTFSSΝCDGSAΝGDTLIKVLWVΝDSAGDSDI 130 140 150 160 170 180 180 190 200 m249. pep SRTNLEVSGDNIVYTYQARVGGREX : I I I I I : : I : I I I I I I I I I I I I I I I a2 9 ARTNLETNGNNIVYTYQARVGGREX 190 200 The following partial DNA sequence was identified in N meningitidis <SEQ ID 973>: 1 ATGAAGAATA ATGATTGCTT CCGCCTGAAA GATTCCCAGT CCGGTATGGC 51 GCTGATAGAA GTCTTGGTTG CTATGCTCGT TCTGACCATC GGTATTTTGG 101 CACTATTGTC TGTACAGTTG CGGACAGTCG CTTCCGTCAG GGAGGCGGAG 151 ACACAAACCA TCGTCAGCCA AATCACGCAA AACCTGATGG AGGGAATGTT 201 GATGAATCCG ACCATTGATT CGGACAGCAA CAAGAAAAAC TATAATCTTT 251 ACATGGGAAA CCATACACTA TCAGCTGTGG ATGGCGATTT TGCGATTGAT 301 GCCATGAAAA CTAAGGGGCA ATTGGCAGAG GCACAATTGA AGAGATTTAG 351 TTATGAGCTG AAAAATGCCT TGCCGGATGC GGCAGCCATC CATTACGCCG 401 TCTGCAAGGA TTCGTCGGGT AACGCGCCGA CATTGTCCGG CAATGCTTTT 451 TCTTCAAATT GCGACAATAA GGCAAACGGG GATACTTTAA TTAAAGTATT 501 GTGGGTAAAT GATTCGGCAG GGGATTCGGA TATTTCCCGT ACGAATCTTG 551 AGGTGAGCGG CGACAATATC GTATATACTT ATCAGGCAAG GGTCGGAGGT 601 CGGGAATGA This corresponds to the amino acid sequence <SEQ ID 974; ORF 249- 1>: m249-l.pep 1 MKΝΝDCFRLK DSQSGMALIE VLVAMLVLTI GILALLSVQL RTVASVREAE 51 TQTIVSQITQ ΝLMEGMLMΝP TIDSDSΝKKΝ YΝLYMGΝHTL SAVDGDFAID 101 AMKTKGQLAE AQLKRFSYEL KΝALPDAAAI HYAVCKDSSG ΝAPTLSGΝAF 151 SSΝCDΝKAΝG DTLIKVLWVΝ DSAGDSDISR TΝLEVSGDΝI VYTYQARVGG 201 RE* m249-l/g249 90.1% identity in 203 aa overlap 10 20 30 40 50 60 m249-1. pep MKΝΝDCFRLKDSQSGMALIEVLVAMLVLTIGILALLΞVQLRTVAΞVREAETQTIVSQITQ I I I I I I : I I h I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g249 MKΝΝDCLRLKΝPQSGMALIEVLVAMLVLTIGILALLΞVQLRTVASVREAETQTIVSQITQ 10 20 30 40 50 60 70 80 90 100 110 120 m249-l.pep ΝLMEGMLMΝPTIDΞDΞΝKKΝYΝLYMGΝHTLSAVDGDFAIDAMKTKGQLAEAQLKRFSYEL I I I I I I I I I I I I I I I I I I I I : I I I I :: I I I I I I I :| :| I hhllll I I I I I I : I I g249 ΝLMEGMLMΝPTIDLDSΝKKΝYSLYMGKQTLΞAVDGEFMLDAEKSKAQLAEEQLKRFSHEL 70 80 90 100 110 120 130 140 150 160 170 179 m249-l.pep KΝALPDAAAIHYAVCKDSSGΝAPTLSGΝ-AFSSΝCDΝKAΝGDTLIKVLWVΝDSAGDSDIS lllll lllhlllll : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g249 KΝALPDAVAIHYAVCKDSSGDAPTLSDSGAFΞSΝCDΝKAΝGDTLIKVLWVΝDSAGDSDIS 130 140 150 160 170 180 180 190 200 m249-1.pep RTNLEVSGDNIVYTYQARVGGREX I I I I I I I I I I I I I I I I I I I I I I I I g249 RTNLEVSGDNIVYTYQARVGGREX 190 200 a249/ L36117 gi I 643582 (L36117) prepilin leader sequence requires cleavage to be active [Pseudomonas aerugmosa] >gι| 1161222 (L48934) involved in type 4 fimbrial biogenesis; contains pre-pilm like leader sequence [Pseudomonas aerugmosa] >gι| 1246299 (L76605) reference L36117, L48934 [Pseudomonas aerugmosa] Length = 185 Score = 50.4 bits (118), Expect = 9e-06 Identities = 45/183 (24%), Positives = 84/183 (45%), Gaps = 26/183 (14%) Query: 13 QSGMALIEVLVAMLVLTIGILALLSVQLRTVASVREAETQTIVSQITQNLMEGMLMNPTI 72 QSG ++IEVLVA+L+++IG+L ++++Q +T+ ++ + + + NL+E M +P Sb 3 Ct: 12 QSGFSMIEVLVALLLISIGVLGMIAMQGKTIQYTADΞVERNKAAMLGSNLLESMRASPKA 71 Query: 73 DΞDΞNKKNYNLYMGNHHALSWDGDFQVDAIKTKTQLAEA QLKRFSYELKNALPDAA 129 D + M G A + T L +A +L ++ ++KN LP A Sbjct: 72 LYDVKDQ MATQSDFFKAKGSAFPTAPΞΞCTPLPDAIKDRLGCWAEQVKNELPGAG 126 Query: 130 Al HYAVCKDSSGVAPTLSAGSTFSSNCDGSANGDTL-IKVLWVNDΞAGDSDIARTNL 185 + Y +C+ S +CDG G L I++ W + A ++ Sbjct: 127 DLLKSDYYICRSSK PGDCDG—KGSMLEIRLAWRGKQGACVNAADSSA 172 Query: 186 ETN 188 +T+ Sbjct: 173 DTS 175 m249-l/a249 90.7% identity in 204 aa overlap 10 20 30 40 50 60 m249-l.pep MKNNDCFRLKDSQSGMALIEVLVAMLVLTIGILALLSVQLRTVASVREAETQTIVSQITQ I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a2 9 MKNNDCFRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVASVREAETQTIVSQITQ 10 20 30 40 50 60 70 80 90 100 110 119 m249-l.pep NLMEGMLMNPTIDSDSNKKNYNLΪMGNH-TLSAVDGDFAIDAMKTKGQLAEAQLKRFSYE I I I I I I I I I I I I I I I I I I I I I I I I I I I I :| hi I I I I : I hi I I I I I I I I I I I I I I I a249 NLMEGMLMNPTIDSDSNKKNYNLYMGNHHALΞWDGDFQVDAIKTKTQLAEAQLKRFSYE 70 80 90 100 110 120 120 130 140 150 160 170 m2 9-l . pep LKNALPDAAAIHYAVCKDSSGNAPTLS-GNAFSΞNCDNKANGDTLIKVLWVNDSAGDΞDI I I I I I I I I I I I I I I I I I I I I I I I I I I I : : I I I I I I : : | I I I I I I I I I I I I I I I I I I I I a249 LKNALPDAAAIHYAVCKDSSGVAPTLSAGSTFSSNCDGSANGDTLIKVLWVNDSAGDSDI 130 140 150 160 170 180 180 190 200 m249-l .pep SRTNLEVSGDNIVYTYQARVGGREX : I I I I h: I: I I I I I I I I I I I I I I I a249 ARTNLETNGNNIVYTYQARVGGREX 190 200 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 975>: g250.seq 1 atgacgcaca cagcctctcc acgtgatgaa ttcatacgcg gcataaaaga 51 aagttcgccc atgctgattg ggcttttgcc ttgggcattg atactcggta 101 tgcagggcgg gcaaaaaggt atgggccggc tggaaatgct gctgatgacg 151 gggatgaact ttgccggcgg ctccgaattt gccacggtca acctgtgggc 201 ggaacctctg ccgatactgc ttatcgccac cataaccttt atgattaatt 251 cgcggcatat cctgatgggg ggcggcgctt gccacgcaca tgaaagaaat 301 accgctgaaa aaagccgcgc ccgcgctgtt ttttatgtgt ga This corresponds to the amino acid sequence <SEQ ED 976; ORF 250.ng>: g250.pep 1 MTHTASPRDE FIRGIKESSP MLIGLLPWAL ILGMQGGQKG MGRLEMLLMT 51 GMΝFAGGSEF ATVΝLWAEPL PILLIATITF MIΝSRHILMG GGACHAHERΝ 101 TAEKSRARAV FYV* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 977>: m250.seq 1 ATGCACACCT TCCCCGCATA ACGAATTTAT ACGCGGCATC AAAGAAAGTT 51 CGCCTATGCT GATTGGGCTG CTGCCTTGGG CATTAATACT CGGTATGCAG 101 GGCGGACAAA AAGGCATGAG CTGGCTGGAA ATGTTGTTGA TGACCAGTAT 151 GAACTTCGCC GGCGGCTCCG AGTTTGCCAC GGTCAACCTG TGGGCsGAAC 201 CTCTGCCGAT ACTGCTTATC GCCACCGTAA CCTTTATGAT TAATTCTCGG 251 CATATCCTGA T.GGGGGCGG CGCTTGCCCC GCACCTGAAA GGAaTACCGC 301 TGAAAAAAGC CGTGCCCGCA CTGTTTTTTA TGTGTGA This corresponds to the amino acid sequence <SEQ ED 978; ORF 250>: m250.pep 1 MHTPSPHΝEF IRGIKESSPM LIGLLPWALI LGMQGGQKGM SWLEMLLMTS 51 MΝFAGGSEFA TVΝLWAEPLP ILLIATVTFM IΝSRHILMGG GACPAPERΝT 101 AEKSRARTVF YV* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 250 shows 91.0% identity over a 111 aa overlap with a predicted ORF (ORF 250.ng) from N gonorrhoeae: m250/g250 10 20 30 40 50 59 m250, .pep MHTPSPHΝEFIRGIKESSPMLIGLLPWALILGMQGGQKGMSWLEMLLMTSMΝFAGGSEF I I I h M M M M M M M M M M M M I M M M I h 1 1 1 1 I I h 1 1 1 1 I I I I I g250 MTHTASPRDEFIRGIKESSPMLIGLLPWALILGMQGGQKGMGRLEMLLMTGMΝFAGGSEF 10 20 30 40 50 60 60 70 80 90 100 110 m250. .pep ATVΝLWAEPLPILLIATVTFMIΝSRHILMGGGACPAPERΝTAEKSRARTVFYVX M M I M M I M M M I I i l l , I g250 ATVΝLWAEPLPILLIATITFMIΝSRHILMGGGACHAHERΝTAEKSRARAVFYV 70 80 90 100 110 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 979>: a250.seq 1 ATGACACACA TAAGCTCGCC CCGTAACGAA TTTATACGCG GCATCAAAGA 51 AAGTTCGCCC ATGCTGATCG GGCTTTTGCC TTGGGCATTA ATACTCGGTA 101 TGCAGGGTGG ACAAAAAGGC ATGAGCTGGC TGGAAATGTT GTTGATGACC 151 GGTATGAACT TCGCCGGCGG CTCCGAGTTT GCCACGGTCA ACCTGTGGGC 201 GGAACCTCTG CCGATACTGC TTATCGCCAC CGTAACCTTT ATGATTAATT 251 CTCGGCATAT CCTGATGGGG G.CGGCACTT GCCCCGCACC TGAAAGAAAT 301 ACCGCTGAAA AAAGCCGTGC CCGCACTGTT TTTTATGTGT GA This corresponds to the amino acid sequence <SEQ ID 980; ORF 250.a>: a250.pep 1 MTHISSPRΝE FIRGIKESSP MLIGLLP AL ILGMQGGQKG MS LEMLLMT 51 GMΝFAGGSEF ATVΝLWAEPL PILLIATVTF MIΝSRHILMG XGTCPAPERΝ 101 TAEKSRARTV FYV* m250/a250 94.6% identity in 111 aa overlap 10 20 30 40 50 59 m250.pep MHTPSPHΝEFIRGIKESSPMLIGLLP ALILGMQGGQKGMS LEMLLMTSMΝFAGGSEF I I I : II I I I I I I I I I I I II I M I I II I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I a250 MTHISSPRΝEFIRGIKESSPMLIGLLP ALILGMQGGQKGMS LEM LMTGMΝFAGGSEF 10 20 30 40 50 60 60 70 80 90 100 110 m250.pep ATVΝL AEPLPILLIATVTFMIΝSRHILMGGGACPAPERΝTAEKSRARTVFYVX I I I I I II I I I I I I I I I I I I I I I I I I II I I I I : I I I II I II I I I II I I I I I I I I a250 ATVΝLWAEPLPILLIATVTFMIΝSRHILMGXGTCPAPERΝTAEKSRARTVFYVX 70 80 90 100 110 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 981>: g251.seq 1 atgcctgacc caatagggat tcttttcgct gccgtcgggg ttgatttttt 51 tgccgttgtt ttgagggggc gttttcaacg aataggcgcg gttggcatgt 101 tgataataat aatcctgatg gcggaggtcg gaaccaaaac ggtcgtaacc 151 gaggttgacg ctcaggttgt ggcggatttt ggcggtatcg aaggattttt 201 tgaatgccgc ctgcaagagc ctgtggcttt ccccgtaaat cacgcggtcg 251 gatttgtagt aggaagacgg cttgtcggca ctcgggcggc aatatttgtc 301 cgaaccgtcg gcggaacagt gcgtctgctg aaaatgattg tccaaaccga 351 tgccctgccg gtcgtaagag aggcgggcat aatccgccca agtgtcttta 401 tcggcattgg tatagacata ttccaaaccg tagcggcttt tggtgtgcgt 451 ctcgtcgtaa aacacgcccg taccgtattc cgcgcccacc tccgcaccgt 501 tttcaccgtt ggtaatcagc ccgctgtatt tgcggccgcc cgcgtatttg 551 ccgtagcctc ttatcgatcc gtatttttta ttttcatcaa aaaccgcctt 601 ggtcaggaat gccggaaccg tcatatcgcg cgtgtcgaaa gtttgctgcg 651 tgcgttcgag tatgccgccg atgtagtgcc gtttgttttc aaaacgaaaa 701 cccgggcgga acagccacga ccggctttcg tatga This corresponds to the amino acid sequence <SEQ ID 982; ORF 251.ng>: g251.pep 1 MPDPIGILFA AVGVDFFA LRGRFQRIGA VGMLIIIILM AEVGTKTWT 51 EVDAQWADF GGIEGFFECR LQEPVAFPVN HAVGFWGRR LVGTRAAIFV 101 RTVGGTVRLL KMIVQTDALP WREAGIIRP SVFIGIGIDI FQTVAAFGVR 151 LWKHARTVF RAHLRTVFTV GNQPAVFAAA RVFAVASYRS VFFIFIKNRL 201 GQECRNRHIA RVESLLRAFE YAADWPFVF KTKTRAEQPR PAFV* The following partial DNA sequence was identified in N meningitidis <SEQ ID 983>: m251. seq 1 ATGCGTGCTG CGGTAGTCGT AGCGCAAGCC CGCGCCGACA TCCGCCCACC 51 TGCCCAAACG GACATTGTCC CGAACTGCCG CGTAATAGCT TTTACCGTTG 101 ATGCTGCGCG GCGTGCAGTC CGTATAAGTA TTGTTGCCCA AGCGGCAGAT 151 TTGCCCCGTA ACGACATTTC CCCTGCCTAT GGTGACCCAA TAGGGGCTGG 201 TTTCACTGCC GTTGGGGCTG ATTTTTTTGC CGTTGTTTTG AGGGGGCGTG 251 TTCGACGAAT AGGCGCGGTT GGCATGTTGA TAATAATAAT CCTGATGGCG 301 GAGATTAGAG CCAAAGCGGT CAAACCCGAG ATTCACGCTC AGGTTGTGGC 351 GGATTTTGGC GGTATCGAAG GATTTTTTGA ATGCCGCCTG CAAGAGCCTG 401 TGGCTTTCCC CGTAAATCAC GCGATCGGAT TTGTAATAGG AAAACGGCTT 451 GTCGGCACTC GGGCGGCAAT ATTTGTCCGA ACCGTCGGCA GAACAGTGCG 501 TCTGCTGAAA ATGATTATCC AAACCGATGC CCTGCCGGTC GTAAGAGAGG 551 CGGGCATAAT CCGCCCAAGT GTCTTTATCG GCATTGGTAT AGACATATTC 601 CAAACCGTAG CGGCTTTTGG TGTGCGTCTC GTCGTAAAAC ACGCCCGTAC 651 CGTATTCCGC GCCCACCAGC GCACCGTTTT CGCCGTTGGT AAACAGTCCG 701 CCGTATTTGT GGTTGCCCGC GTATTTGCCG TTACCGGGCA AAGAACCCGC 751 CTGTTTTTTA TTTGCATCAA AAACCGCCTT GGTCAGGAAT GCCGGAACCG 801 TCATATCGCG CGTGTCGAAA GTTTGTTGCG TGTGTTCGAG TATGCCGCCG 851 ATGTAGTGCC GCTTATTCTC AAAACGAAAA CCCGGGCGGA ACAGCCACGA 901 CCGGCTTTCG TATGA This corresponds to the amino acid sequence <SEQ ID 984; ORF 251>: m251.pep 1 MRAAVWAQA RADIRPPAQT DIVPΝCRVIA FTVDAARRAV RISIVAQAAD 51 LPRΝDISPAY GDPIGAGFTA VGADFFAWL RGRVRRIGAV GMLIIIILMA 101 EIRAKAVKPE IHAQWADFG GIEGFFECRL QEPVAFPVΝH AIGFVIGKRL 151 VGTRAAIFVR TVGRTVRLLK MIIQTDALPV VREAGIIRPS VFIGIGIDIF 201 QTVAAFGVRL WKHARTVFR AHQRTVFAVG KQSAVFWAR VFAVTGQRTR 251 LFFICIKΝRL GQECRNRHIA RVESLLRVFE YAADWPLIL KTKTRAEQPR 301 PAFV* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 251 shows 85.2% identity over a 243 aa overlap with a predicted ORF (ORF 251.ng) from N gonorrhoeae: m25l/g251 40 50 60 70 80 90 m251.pep TVDAARRAVRISIVAQAADLPRΝDISPAYGDPIGAGFTAVGADFFAWLRGRVRRIGAVG M M h l l h l l l l l l l l l l M l l l l l g251 MPDPIGILFAAVGVDFFAWLRGRFQRIGAVG 10 20 30 100 110 120 130 140 150 m251.pep MLIIIILMAEIRAKAVKPEIHAQWADFGGIEGFFECRLQEPVAFPVNHAIGFVIGKRLV 1111111 M I = -- '-l h g251 MLI 11 ILMAEVGTKTWTEVDAQWADFGGIEGFFECRLQEPVAFPVNHAVGFWGRRLV 40 50 60 70 80 90 160 170 180 190 200 210 m251.pep GTRAAIFVRTVGRTVRLLKMIIQTDALPWREAGIIRPSVFIGIGIDIFQTVAAFGVRLV 111111111111 MMMIhlMMMMMMMMMMMMMMMMMMI g251 GTRAAIFVRTVGGTVRLLKMIVQTDALPWREAGIIRPSVFIGIGIDIFQTVAAFGVRLV 100 110 . 120 130 140 150 220 230 240 250 260 270 m251.pep VKHARTVFRAHQRTVFAVGKQSAVFWARVFAVTGQRTRLFFICIKNRLGQECRNRHIAR I iMM IM 111 ::] 11111 :: h MM MMMMMMMM g251 VKHARTVFRAHLRTVFTVGNQPAVFAAARVFAVASYRS-VFFIFIKNRLGQECRNRHIAR 160 170 180 190 200 210 280 290 300 m251. pep VESLLRVFEYAADWPLILKTKTRAEQPRPAFVX MMIhMIIMMh MIIMIMMIMM g251 VESLLRAFEYAADWPFVFKTKTRAEQPRPAFVX 220 230 240 The following partial DNA sequence was identified in N meningitidis <SEQ ID 985>: a251. seq 1 ATGCGTGCTG CGGTAGTCGT AGCGCAACCC CGCGCCGACA TCCGCCCACC 51 TGCCCAAACG GACATTGTCC CGAACTGCCG CGTAATAGCT TTTGCCGTTG 101 ATGCTGCGCG GCGTGCAGTC CGTATAAGTA TTGTTGCCCA AGCGGCAGAT 151 TTGCCCCGTA ACCACATTTC CCCTGCCTAT GCTGACCCAA TAGGGTTGGT 201 CCTTGCCGCC GTTGGGGTTG GCGGTTTTAG GGGGCGTTTT CGACGAATAG 251 GCGCGGTTGG CATGTTGATA ATAATAATCC TGATGGCGGA GATTAGAGTC 301 AAAGCGGTCA AAACCGAGAT TCACGCTCAG GTTGTGGCGG ATTTTGGCGG 351 TATCGAAGGA TTTTTTGAAT GCCGCCTGCA AGAGCCTGTG GCTTTCCCCG 401 TAAATCACGC GGTCGGATTT GTAGTAGGAA AACGGCTTGT CGGCACTCGG 451 GCGGCAATAT TTGTCCGAAC CGTCGGCAGA ACAGTGCGTC TGCTGAAAAT 501 GATTGTCCAA ACCGATGCCC TGCCGGTCGT AAGAGAGGCG GGCATAATCC 551 ACCCAAGTGT CTTTATCGGC ATTGGTATAG ACATATTCCA AACCGTAGCG 601 GCTTTTGGTG TGCGTCTCGT CGTAAAACAC GCCCGTACCG TATTCCGCGC 651 CCACCAGCGC ACCGTTTTCG CCGTTGGTAA ACAGACCGCC GTATTTGTGG 701 TCGCCCGCGT ATTTGCCGTT GCCTCTTATC GGTCCGTATT TTCTATTTTC 751 ATCAAAAACC GCCTTGGTCA GGAATGCCGG AACCGTCATA TCGCGCGTGT 801 CGAAAGTTTG TTGCGTGTGT TCGAGTATGC CGCCGATGTA GTGCCGTTTG 851 TTTTCAAAAC GAAAACCCGG GCGGAACAGC CACGATCGGC TTTCGTATGA This corresponds to the amino acid sequence <SEQ ID 986; ORF 251.a>: a251.pep 1 MRAAVWAQP RADIRPPAQT DIVPΝCRVIA FAVDAARRAV RISIVAQAAD 51 LPRΝHISPAY ADPIGLVLAA VGVGGFRGRF RRIGAVGMLI IIILMAEIRV 101 KAVKTEIHAQ ADFGGIEG FFECRLQEPV AFPVΝHAVGF WGKRLVGTR 151 AAIFVRTVGR TVRLLKMIVQ TDALPWREA GIIHPSVFIG IGIDIFQTVA 201 AFGVRLWKH ARTVFRAHQR TVFAVGKQTA VFWARVFAV ASYRSVFSIF 251 IKΝRLGQECR ΝRHIARVESL LRVFEYAADV VPFVFKTKTR AEQPRSAFV* m25 l/a251 88.5% identity in 304 aa overlap 10 20 30 40 50 60 m251. pep MRAAWVAQARADIRPPAQTDIVPΝCRVIAFTVDAARRAVRISIVAQAADLPRΝDISPAY l l l l l l l l l I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I a251 MRAAVWAQPRADIRPPAQTDIVPΝCRVIAFAVDAARRAVRISIVAQAADLPRΝHISPAY 10 20 30 40 50 60 70 80 90 100 110 120 m251. pep GDPIGAGFTAVGADFFAWLRGRVRRIGAVGMLIIIILMAEIRAKAVKPEIHAQWADFG : I I I I :: I I I : I III I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I a251 ADPIGLVLAAVGVGGF RGRFRRIGAVGMLIIIILMAEIRVKAVKTEIHAQWADFG 70 80 90 100 110 130 140 150 160 170 180 m251. pep GIEGFFECRLQEPVAFPVNHAIGFVIGKRLVGTRAAIFVRTVGRTVRLLKMIIQTDALPV I I I I I I I I I I I I I I I I I I I I I : I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I a251 GIEGFFECRLQEPVAFPVNHAVGFWGKRLVGTRAAIFVRTVGRTVRLLKMIVQTDALPV 120 130 140 150 160 170 190 200 210 220 230 240 m251. pep VREAGIIRPSVFIGIGIDIFQTVAAFGVRLWKHARTVFRAHQRTVFAVGKQSAVFWAR I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II: I I I I I I I a251 VREAGIIHPSVFIGIGIDIFQTVAAFGVRLWKHARTVFRAHQRTVFAVGKQTAVFWAR 180 190 200 210 220 230 250 260 270 280 290 300 m251. pep VFAVTGQRTRLFFICIKNRLGQECRNRHIARVESLLRVFEYAADWPLILKTKTRAEQPR MM:: I : : I I I I I II I II I II II II I I I I II I I II II I I I II ::: I II II II I II a251 VFAVASYRS-VFSIFIKNRLGQECRNRHIARVESLLRVFEYAADWPFVFKTKTRAEQPR 240 250 260 270 280 290 m251. .pep PAFVX 1111 a251 SAFVX 300 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 987>: g253.seq 1 atgatcgaca gggaccgtat gttgcgggac acgttggaac gtgtgcgtgc 51 ggggtcgttc tggttatggg tggtggtggc atcgatgatg tttaccgccg 101 gattttcagg cacttatctt ctgatggaca atcaggggct gaatttcttt 151 ttagttttgg cgggagtgtt gggcatgaat acgctgatgc tggcagtatg 201 gttggcaacg ttgttcctgc gcgtgaaagt gggacggttt ttcagcagtc 251 cggcgacgtg gtttcggggc aaaggccctg taaatcaggc ggtgttgcgg 301 ctgtatgcgg accagtggcg gcaaccttcg gtacgatgga aaataggcgc 351 aacggcgcac agcttgtggc tctgcacgct gctcggaatg ctggtgtcgg 401 tattgctgct gcttttggtg cggcaatata cgttcaactg ggaaagcacg 451 ctgttgagca atgccgcttc ggtacgcgcg gtggaaatgt tggcatggct 501 gccgtcgaaa ctcggtttcc ctgtccccga tgcgcgggcg gtcatcgaag 551 gtcgtctgaa cggcaatatt gccgatgcgc gggcttggtc ggggctgctg 601 gtcggcagta tcgtctgcta cggcatcctg ccgcgcctct tggcttgggt 651 agtgtgtaaa atccttttga aaacaagcga aaacggattg gatttggaaa 701 aaacctatta tcaggcggtc atccgccgct ggcagaacaa aatcaccgat 751 gcggatacgc gtcgggaaac cgtgtccgcc gtttcgccga aaatcgtctt 801 gaacgatgcg ccgaaatggg cgctcatgct ggagaccgag tggcaggacg 851 gccaatggtt cgagggcagg ctggcgcagg aatggctgga taagggcgtt 901 gccgccaatc gggaacaggt tgccgcgctg gagacagagc tgaagcagaa 951 accggcgcaa ctgcttatcg gcgtacgcgc ccaaactgtg ccggaccggg 1001 gcgtgctgcg gcagattgtg cggctttcgg aagcggcgca gggcggcgcg 1051 gtggtgcagc ttttggcgga acaggggctt tcagacgacc tttcggaaaa 1101 gctggaacat tggcgtaacg cgctgaccga atgcggcgcg gcgtggcttg 1151 agcctgacag ggtggcgcag gaaggccgtt tgaaagacca ataa This corresponds to the amino acid sequence <SEQ ID 988; ORF 253. ng>: g253.pep 1 MIDRDRMLRD TLERVRAGSF WLWVWASMM FTAGFSGTYL LMDΝQGLΝFF 51 LVLAGVLGMΝ TLMLAVWLAT LFLRVKVGRF FSSPATWFRG KGPVΝQAVLR 101 LYADQWRQPS VRWKIGATAH SLWLCTLLGM LVSVLLLLLV RQYTFΝWEST 151 LLSΝAASVRA VEMLAWLPSK LGFPVPDARA VIEGRLΝGΝI ADARAWSGLL 201 VGSIVCYGIL PRLLAWWCK ILLKTSEΝGL DLEKTYYQAV IRRWQΝKITD 251 ADTRRETVSA VSPKIVLΝDA PKWALMLETE WQDGQWFEGR LAQEWLDKGV 301 AANREQVAAL ETELKQKPAQ LLIGVRAQTV PDRGVLRQIV RLSEAAQGGA 351 WQLLAEQGL SDDLSEKLEH WRNALTECGA AWLEPDRVAQ EGRLKDQ* The following partial DNA sequence was identified in N meningitidis <SEQ ID 989>: m253. seq 1 ATGATTGACA GGAACCGTAT GCTGCGGGAG ACGTTGGAAC GTGTGCGTGC 51 GGGGTCGTTC TGGTTGTGGG TGGTGGCGGC GACGTTTGCA TTTTTTACCG 101 GTTTTTCAGT CACTTATCTT CTAATGGACA ATCAGGGTCT GAATTTCTTT 151 TTGGTTTTGG CGGGCGTGTT GGGCATGAAT ACGCTGATGC TGGCAGTATG 201 GTTGGCAATG TTGTTCCTGC GTGTGAAAGT GGGGCGTTTT TTCAGCAGTC 251 CGGCGACGTG GTTTCGGGGC AAAGACCCTG TAAATCAGGC GGTGTTGCGG 301 CTGTATGCGG ACGAGTGGCG GCAACCTTCG GTACGTTGGA AAATAGGCGC 351 AACGTCGCAC AGCCTGTGGC TCTGCACGCT GCTCGGAATG CTGGTGTCGG 401 TATTGTTGCT GCTTTTGGTG CGGCAATATA CGTTCAACTG GGAAAGCACG 451 CTGTTGAGCA ATGCCGCTTC GGTACGCGCG GTGGAAATGT TGGCATGGCT 501 GCCGTCGAAA CTCGGTTTCC CTGTCCCCGA TGCGCGGGCG GTCATCGAAG 551 GCCGTCTGAA CGGCAATATT GCCGATGCGC GGGCTTGGTC GGGGCTGCTG 601 GTCGGCAGTA TCGCCTGCTA CGGCATCCTG CCGCGCCTGC TGGCTTGGGT 651 AGTGTGTAAA ATCCTTTTGA AAACAAGCGA AAACGGATTG GATTTGGAAA 701 AGCCCTATTA TCAGGCGGTC ATCCGCCGCT GGCAGAACAA AATCACCGAT 751 GCGGATACGC GTCGGGAAAC CGTGTCCGCC GTTTCACCGA AAATCATCTT 801 GAACGATGCG CCGAAATGGG CGGTCATGCT GGAGACCGAG TGGCAGGACG 851 GCGAATGGTT CGAGGGCAGG CTGGCGCAGG AATGGCTGGA TAAGGGCGTT 901 GCCACCAATC GGGAACAGGT TGCCGCGCTG GAGACAGAGC TGAAGCAGAA 951 ACCGGCGCAA CTGCTTATCG GCGTGCGCGC CCAAACTGTG CCGGACCGCG 1001 GCGTGTTGCG GCAGATTGTC CGACTCTCGG AAGCGGCGCA GGGCGGCGCG 1051 GTGGTGCAGC TTTTGGCGGA ACAGGGGCTT TCAGACGACC TTTCGGAAAA 1101 GCTGGAACAT TGGCGTAACG CGCTGGCCGA ATGCGGCGCG GCGTGGCTTG 1151 AGCCTGACAG GGCGGCGCAG GAAGGGCGTT TGAAAGACCA ATAA This corresponds to the amino acid sequence <SEQ ID 990; ORF 253>: m253.pep 1 MIDRΝRMLRE TLERVRAGSF WLWWAATFA FFTGFSVTYL LMDΝQGLΝFF 51 LVLAGVLGMΝ TLMLAVWLAM LFLRVKVGRF FSSPATWFRG KDPVΝQAVLR 101 LYADEWRQPS VRWKIGATSH SLWLCTLLGM LVSVLLLLLV RQYTFΝWEST 151 LLSΝAASVRA VEMLAWLPSK LGFPVPDARA VIEGRLΝGΝI ADARAWSGLL 201 VGSIACYGIL PRLLAWWCK ILLKTSEΝGL DLEKPYYQAV IRRWQΝKITD 251 ADTRRETVSA VSPKIILΝDA PKWAVMLETE WQDGEWFEGR LAQEWLDKGV 301 ATΝREQVAAL ETELKQKPAQ LLIGVRAQTV PDRGVLRQIV RLSEAAQGGA 351 WQLLAEQGL SDDLSEKLEH WRΝALAECGA AWLEPDRAAQ EGRLKDQ* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 253 shows 94.7% identity over a 397 aa overlap with a predicted ORF (ORF 253.ng) from N gonorrhoeae: m253/g253 10 20 30 40 50 60 m253.pep MIDRΝRMLRETLERVRAGSFWLWWAATFAFFTGFSVTYLLMDΝQGLΝFFLVLAGVLGMΝ M l h M l h M M M I M M M I h h : I M M I I 1 1 1 1 1 I I I I I I 1 1 1 I I I I I I I g253 MIDRDRMLRDTLERVRAGSFWLWVWASMMFTAGFSGTYLLMDΝQGLΝFFLVLAGVLGMΝ 10 20 30 40 50 60 70 80 90 100 110 120 m253. pep TLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVΝQAVLRLYADEWRQPSVRWKIGATSH M I M I I I I M M M M M M M I M M M 1 1 I I I I I I I I I h I I 1 1 I I 1 1 1 I I I h I g253 TLMLAVWLATLFLRVKVGRFFSSPATWFRGKGPVΝQAVLRLYADQWRQPSVRWKIGATAH 70 80 90 100 110 120 130 140 150 160 170 180 m253.pep SLWLCTLLGMLVSVLLLLLVRQYTFΝWESTLLSΝAASVRAVEMLAWLPSKLGFPVPDARA MMMMMMMIMMMMMMMMMMMMMMMMMMIMMIMI g253 SLWLCTLLGMLVSVLLLLLVRQYTFΝWESTLLSΝAASVRAVEMLAWLPSKLGFPVPDARA 130 140 150 160 170 180 190 200 210 220 230 240 m253.pep VIEGRLNGNIADARAWSGLLVGSIACYGILPRLLAWWCKILLKTSENGLDLEKPYYQAV Mill g253 VIEGRLNGNIADARAWSGLLVGS IVCYGI LPRLLAWWCKILLKTSENGLDLEKTYYQAV 190 200 210 220 230 240 250 260 270 280 290 300 m253 . pep IRRWQNKITDADTRRETVSAVSPKI ILNDAPKWAVMLETEWQDGEWFEGRLAQEWLDKGV MMMMMMMMMMMMhMMMIhMMMMhMMMMIMMM g253 IRRWQNKITDADTRRETVSAVSPKIVLNDAPKWALMLETEWQDGQWFEGRLAQEWLDKGV 250 260 270 280 290 300 310 320 330 340 350 360 m253.pep ATNREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLSEAAQGGAWQLLAEQGL hMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g253 AANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLSEAAQGGAWQLLAEQGL 310 320 330 340 350 360 370 380 390 m253. pep SDDLSEKLEHWRNALAECGAAWLEPDRAAQEGRLKDQX 1111111111 M i ! I = i 11111111 ! 1 : 111 i 111111 g253 SDDLSEKLEHWRNALTECGAAWLEPDRVAQEGRLKDQX 370 380 390 The following partial DNA sequence was identified in N meningitidis <SEQ ID 991>: a253. seq 1 ATGATCGACA GGAACCGTAT GCTGCGGGAG ACGTTGGAAC GTGTGCGTGC 51 GGGGTCGTTC TGGTTGTGGG TGGCGGCGGC GACGTTTGCG TTTTTTACCG 101 GTTTTTCAGT TACTTATCTT CTAATGGACA ATCAGGGTCT GAATTTCTTT 151 TTGGTTTTGG CGGGCGTGTT GGGCATGAAT ACGCTGATGC TGGCAGTATG 201 GTTGGCAATG TTGTTCCTGC GCGTGAAAGT GGGGCGTTTT TTCAGCAGTC 251 CGGCGACGTG GTTTCGGGGC AAAGACCCTG TCAATCAGGC GGTGTTGCGG 301 CTGTATGCGG ACGAGTGGCG GCAACCTTCG GTACGTTGGA AAATAGGCGC 351 AACGTCGCAC AGCCTGTGGC TCTGCACGCT GCTCGGAATG CTGGTGTCGG 401 TATTGTTGCT GCTTTTGGTG CGGCAATATA CGTTCAACTG GGAAAGCACG 451 CTGTTGGGCG ATTCGTCTTC GGTACGGCTG GTGGAAATGT TGGCATGGCT 501 GCCTGCGAAA CTGGGTTTTC CCGTGCCTGA TGCGCGGGCG GTCATCGAAG 551 GTCGTCTGAA CGGCAATATT GCCGATGCGC GGGCTTGGTC GGGGCTGCTG 601 GTCGGCAGTA TCGCCTGCTA CGGCATCCTG CCGCGCCTCT TGGCTTGGGC 651 GGTATGCAAA ATCCTTTTGA AAACAAGCGA AAACGGCTTG GATTTGGAAA 701 AGCCCTATTA TCAGGCGGTC ATCCGCCGCT GGCAGAACAA AATCACCGAT 751 GCGGATACGC GTCGGGAAAC CGTGTCCGCC GTTTCGCCGA AAATCGTCTT 801 GAACGATGCG CCGAAATGGG CGGTCATGCT GGAGACCGAA TGGCAGGACG 851 GCGAATGGTT CGAGGGCAGG CTGGCGCAGG AATGGCTGGA TAAGGGCGTT 901 GCCGCCAATC GGGAACAGGT TGCCGCGCTG GAGACAGAGC TGAAGCAGAA 951 ACCGGCGCAA CTGCTTATCG GCGTGCGCGC CCAAACTGTG CCCGACCGCG 1001 GCGTGTTGCG GCAGATCGTC CGACTTTCGG AAGCGGCGCA GGGCGGCGCG 1051 GTGGTGCAGC TTTTGGCGGA ACAGGGGCTT TCAGACGACC TTTCGGAAAA 1101 GCTGGAACAT TGGCGTAACG CGCTGACCGA ATGCGGCGCG GCGTGGCTGG 1151 AACCCGACAG AGCGGCGCAG GAAGGCCGTC TGAAAACCAA CGACCGCACT 1201 TGA This corresponds to the amino acid sequence <SEQ ID 992; ORF 253. a>: a253.pep 1 MIDRΝRMLRE TLERVRAGSF WLWVAAATFA FFTGFSVTYL LMDΝQGLΝFF 51 LVLAGVLGMΝ TLMLAVWLAM LFLRVKVGRF FSSPATWFRG KDPVΝQAVLR 101 LYADEWRQPS VRWKIGATSH SLWLCTLLGM LVSVLLLLLV RQYTFΝWEST 151 LLGDSSSVRL VEMLAWLPAK LGFPVPDARA VIEGRLΝGΝI ADARAWSGLL 201 VGSIACYGIL PRLLAWAVCK ILLKTSEΝGL DLEKPYYQAV IRRWQΝKITD 251 ADTRRETVSA VSPKIVLΝDA PKWAVMLETE WQDGEWFEGR LAQEWLDKGV 301 AAΝREQVAAL ETELKQKPAQ LLIGVRAQTV PDRGVLRQIV RLSEAAQGGA 351 WQLLAEQGL SDDLSEKLEH WRΝALTECGA AWLEPDRAAQ EGRLKTΝDRT 401 * m253/a253 97.2% identity in 395 aa overlap 10 20 30 40 50 60 m253 . pep MIDRNRMLRETLERVRAGSFWLWWAATFAFFTGFSVTYLLMDNQGLNFFLVLAGVLGMN I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a253 MIDRNRMLRETLERVRAGSFWLWVAAATFAFFTGFSVTYLLMDNQGLNFFLVLAGVLGMN 10 20 30 40 50 60 70 80 90 100 110 120 m253. pep TLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAVLRLYADEWRQPSVRWKIGATSH I I II I II I I II II I I II II I II I II I II I II II II I II II I II I II II I II II II I II II a253 TLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAVLRLYADEWRQPSVRWKIGATSH 70 80 90 100 110 120 130 140 150 160 170 180 m253. pep SLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLSNAASVRAVEMLAWLPSKLGFPVPDARA I II II II I I II I I I I II II II I I II I I I II II ::: : I I I II I I II II : I I II I I II I II a253 SLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLGDSSSVRLVEMLAWLPAKLGFPVPDARA 130 140 150 160 170 180 190 200 210 220 230 240 m253. pep VIEGRLNGNIADARAWSGLLVGSIACYGILPRLLAWVVCKILLKTSENGLDLEKPYYQAV I II I II II I II I I II I II II I I II I II II I II II I I : I I I II II II II II I I II II I II I a253 VIEGRLNGNIADARAWSGLLVGSIACYGILPRLLAWAVCKILLKTSENGLDLEKPYYQAV 190 200 210 220 230 240 250 260 270 280 290 300 m253. pep IRRWQNKITDADTRRETVSAVSPKIILNDAPKWAVMLETEWQDGEWFEGRLAQEWLDKGV I I II II I II I II II I I II II II II I: I II II I I I I I I II II II I I II II II I II I I II I I a253 IRRWQNKITDADTRRETVSAVSPKIVLNDAPKWAVMLETEWQDGEWFEGRLAQEWLDKGV 250 260 270 280 290 300 310 320 330 340 350 360 m253. pep ATNREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLSEAAQGGAWQLLAEQGL I : I I I II I I II I I II I II II I I II II I II II I II I II I I I II II II II II I I II II II I I a253 AANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLSEAAQGGAWQLLAEQGL 310 320 330 340 350 360 370 380 390 m253.pep SDDLSEKLEHWRNALAECGAAWLEPDRAAQEGRLKDQX I II I II I II I II I I I : I II I II I II I II I I I II I I a253 SDDLSEKLEHWRNALTECGAAWLEPDRAAQEGRLKTNDRTX 370 380 390 400 The following partial DNA sequence was identified in N gonorrhoeae <SEQ TD 993>: g254. seq 1 atgtatgcag gcgaacgctt caatacttac agccatttga gcggtttgat 51 tctggcggcg gcaggtttga tgctgatgct gctgaaaacc ataggacacg 101 gggacggata ccgtatcttc agcgtatcgg tttacggcat cagccttctt 151 ctgctctatt tgagttcctc gctgtaccac ggaattgcag ccggaaaact 201 gaaaagcatt ttgaaaaaaa ccgaccactg catgatttat gtgctgattg 251 ccggaagcta cacaccgttt gcactggttt ctttgagaaa cgggccgggc 301 tggacggtat tttcactgtc ctggctgctg gcggctgcag gaatcgcaca 351 agaactcacc atcggacgga aaagcgaaaa acgtctgctg tctattgcga 401 tttatatcgt aatgggctgg atggtcttgg cggtaatgaa atccctgaca 451 gcctcactcc cgccggcagg actggcttgg ctggcggcag gcggtatgct 501 gtacagcgtc ggcatttact ggtttgtaaa cgatgaaaaa atccgacacg 551 ggcacggaat ctggcatctg ttcgtattgg gcggcagcat aacccaattt 601 gtcagcgtgt acggttatgt aatctga This corresponds to the amino acid sequence <SEQ ID 994; ORF 254.ng>: g254.pep 1 MYAGERFΝTY SHLSGLILAA AGLMLMLLKT IGHGDGYRIF SVSVYGISLL 51 LLYLSSSLYH GIAAGKLKSI LKKTDHCMIY VLIAGSYTPF ALVSLRNGPG 101 WTVFSLSWLL AAAGIAQELT IGRKSEKRLL SIAIYIVMGW MVLAVMKSLT 151 ASLPPAGLAW LAAGGMLYSV GIYWFVNDEK IRHGHGIWHL FVLGGSITQF 201 VSVYGYVI* The following partial DNA sequence was identified in N meningitidis <SEQ ED 995>: m254.seq (partial) 1 ..GTATCGGTTT ACGGCATCAG CCTTCTTCTG CTCTATTTGA GTTCCTGGCT 51 GTACCACGGA ATTGCAGCCG GAAAACTGAA AAGCATTTTG AAAAAAACCG 101 ACCACTGCAT GATTTATGTG CTGATTGCCG GAAGCTACAC ACCGTTTGCA 151 CTGGTTTCTT TGAGAAACGG GCCGGGCTGG ACGGTATTTT CACTGTCCTG 201 GCTGCTGGCG GCTGCAGGAA TCGCACAAGA ACTCACCATC GGACGGAAAA 251 GCGAAAAACG TCTGCTGTCT ATTGTGATTT ATGTCGTCAT GGGTTGGATG 301 GTCTTGGCGG TAATGAAATC CCTGACAGCC TCACTCCCGT CGGCAGGACT 351 GGCTTGGCTG GCGGCAGGCG GTATGCTGTA CAGTGTCGGC ATTTACTGGT 401 TTGTAAACGA TGAAAAAATC CGACACGGGC ACGGAATCTG GCATCTGTTC 451 GTATTGGGCG GCAGCATCAC CCAATTTGTC AGCGTGTACG GTTACGTAAT 501 CTGA This corresponds to the amino acid sequence <SEQ ID 996; ORF 254>: m254.pep (partial) 1 ..VSVYGISLLL LYLSSWLYHG IAAGKLKSIL KKTDHCMIYV LIAGSYTPFA 51 LVSLRΝGPGW TVFSLSWLLA AAGIAQELTI GRKSEKRLLS IVIYWMGWM 101 VLAVMKSLTA SLPSAGLAWL AAGGMLYSVG IYWFVΝDEKI RHGHGIWHLF 151 VLGGSITQFV SVYGYVI* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 254 shows 97.6% identity over a 167 aa overlap with a predicted ORF (ORF 254.ng) from N. gonorrhoeae: m254/g254 10 20 30 m254. pep VSVYGISLLLLYLSSWLYHGIAAGKLKSIL M M M M I M M M l l l l l l l l l l l l l l g254 HLSGLILAAAGLMLMLLKTIGHGDGYRIFSVSVYGISLLLLYLSSSLYHGIAAGKLKSIL 20 30 40 50 60 70 40 50 60 70 80 90 m254.pep KKTDHCMIYVLIAGSYTPFALVSLRΝGPGWTVFSLSWLLAAAGIAQELTIGRKSEKRLLS MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g254 KKTDHCMIYVLIAGSYTPFALVSLRΝGPGWTVFSLSWLLAAAGIAQELTIGRKSEKRLLS 80 90 100 110 120 130 100 110 120 130 140 150 m254 . pep IVI YWMGWMVLAVMKSLTASLPSAGLAWLAAGGMLYSVGI YWFVΝDEKIRHGHGIWHLF h l h l M M M M I M M M M M I I I I I I I I I I I I 1 1 1 1 I I I I I I I I I 1 1 I I I I I I I g254 IAIYIVMGWMVLAVMKSLTASLPPAGLAWLAAGGMLYSVGIYWFVΝDEKIRHGHGIWHLF 140 150 160 170 180 190 160 m254.pep VLGGSITQFVSVYGYVIX I I I I I II II I II I I II I I g254 VLGGSITQFVSVYGYVIX 200 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 997>: a254. seq 1 ATGTATACAG GCGAACGCTT CAATACTTAC AGCCATTTGA GCGGTTTGAT 51 TCTGGCGGCG GCAGGTTTGG CGCTGATGCT GCTGAAAACC ATAGGACACG 101 GGGACGGCTA CCGTATCTTC AGCGTATCGG TTTACGGCAT CAGCCTTCTT 151 CTGCTCTATT TGAGTTCCTC GCTGTACCAC GGAATTGCAG CCGGAAAACT 201 GAAAAGCATT TTGAAAAAAA CCGACCACTG CATGATTTAT GTGCTGATTG 251 CCGGAAGCTA CACACCGTTT GCACTGGTTT CTTTGAGAAA CGGGCCGGGC 301 TGGACGGTAT TTTCACTGTC CTGGCTGCTG GCGGCTGCAG GAATCGCACA 351 AGAACTCACC ATTGGACGGA AAAGCGAAAA ACGACTGCTG TCTATTGCGA 401 TTTATATCGT AATGGGCTGG ATGGTCTTGG CGGTAATGAA ATCCCTGACA 451 GCCTCACTCC CGCCGGCAGG ACTGGCTTGG CTGGCGGCAG GCGGTATGCT 501 GTACAGCGTC GGCATTTACT GGTTTGTAAA CGATGAAAAA ATCCGACACG 551 GGCACGGAAT CTGGCATCTG TTCGTATTGG GCGGCAGCAT CACCCAATTT 601 GTCAGCGTGT ACGGTTACGT AATCTGA This corresponds to the amino acid sequence <SEQ ID 998; ORF 254. a>: a254.pep 1 MYTGERFNTY SHLSGLILAA AGLALMLLKT IGHGDGYRIF SVSVYGISLL 51 LLYLSSSLYH GIAAGKLKSI LKKTDHCMIY VLIAGSYTPF ALVSLRNGPG 101 WTVFSLSWLL AAAGIAQELT IGRKSEKRLL SIAIYIVMGW MVLAVMKSLT 151 ASLPPAGLAW LAAGGMLYSV GIYWFVNDEK IRHGHGIWHL FVLGGSITQF 201 VSVYGYVI* m254/a254 97.6% identity in 167 aa overlap 10 20 30 m254.pep VSVYGISLLLLYLSSWLYHGIAAGKLKSIL a254 HLSGLILAAAGLALMLLKTIGHGDGYRIFSVSVYGISLLLLYLSSSLYHGIAAGKLKSIL 20 30 40 50 60 70 40 50 60 70 80 90 m254.pep KKTDHCMIYVLIAGSYTPFALVSLRNGPGWTVFSLSWLLAAAGIAQELTIGRKSEKRLLS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a254 KKTDHCMIYVLIAGSYTPFALVSLRNGPGWTVFSLSWLLAAAGIAQELTIGRKSEKRLLS 80 90 100 110 120 130 100 110 120 130 140 150 m254.pep IVIYVVMGWMVLAVMKSLTASLPSAGLAWLAAGGMLYSVGIYWFVNDEKIRHGHGIWHLF I : I I : I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a254 IAIYIVMGWMVLAVMKSLTASLPPAGLAWLAAGGMLYSVGIYWFVNDEKIRHGHGIWHLF 140 150 160 170 180 190 160 m254.pep VLGGSITQFVSVYGYVIX I I I I I I I I I I I I I I I I I I a254 VLGGSITQFVSVYGYVIX 200 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 999>: g255. seq 1 atggttggac aggaagcctt gcggggtcag ttcgtcgccg tgttcgctgc 51 cgcgttgcgt tacgctgtca aaacctgcgc cgatttccac gcctttgacg 101 gcgttgatgc ccatcatcgc gtaggcgatt tcggcatcga ggcggtcgaa 151 aacgggttcg cccaaaccga cggggacgtt ggcggcttcg atatgcagtt 201 tcgcgccgac ggaatccaag gatttgcgca caccgtccat atagtgttcc 251 agttcggcga tttggctttg gttggcggca aaaaaaggat tttgggaaat 301 gtgttcgctg ccttcaaacc ggattttttt ttcgccgact tgggtaacgt 351 aggcggtgat ttccgtgccg aatttttctt tcagccattt tttggcaacg 401 gctccggcgg caacgcgggc tgcggtttcg cgggcggaac tcctgccgcc 451 gccccggtag tcgcgcgtac cgtatttgtg ccaataggta tagtcggcgt 501 gtccggggcg gaaggcggtg gcgatgtcgc cgtagtcttc gctgcgctgg 551 tcggtgttgc ggattag This corresponds to the amino acid sequence <SEQ ID 1000; ORF 255.ng>: g255.pep 1 MVGQEALRGQ FVAVFAAALR YAVKTCADFH AFDGVDAHHR VGDFGIEAVE 51 NGFAQTDGDV GGFDMQFRAD GIQGFAHTVH IVFQFGDLAL VGGKKRILGN 101 VFAAFKPDFF FADLGNVGGD FRAEFFFQPF FGNGSGGNAG CGFAGGTPAA 151 APWARTVFV PIGIVGVSGA EGGGDVAWF AALVGVAD* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1001>: m255.seq 1 GTGGTTGGAC AGGAAGCCTT GCGGGGTCAG TTCGTCGCCG TGTTCGCTGC 51 CGCGTTGCGT TACGCTGTCA AAACCTGCGC CGATTTCCAC GCCTTTGACG 101 GCGTTGATGC CCATCATCGC GTAGGCGATT TCGGCATCGA GGCGGTCAAA 151 AACAGGTTCG CCCAAGCCGA CAGGGACATT GGCTGCTTCG ATATGCAGCT 201 TCGCGCCGAC GGAATCCAAG GATTTGCGCA CGCTGTCCAT ATAGTTTTCC 251 AGCTCGGCAA TTTGGCTATG GTTGGCGGCA AAAAAAGGAT TTTGGGAAAT 301 GTGTTCGCAG CCTTCAAACC GGATTTCTTT TTCGCCGACT TGGGTAACGT 351 AGGCGGTGAT TTCCGTGCCG AATTTTTCTT TCAACCATTT TTTGGCAACG 401 GCTCCGGCAG CAACGCGGGC GGCGGTTTCA CGGGCGGAGC TCCTGCCGCC 451 GCCGCGGTAG TCGCGCGTGC CGTATTTGTG CCAATAGGTA TAGTCGGCGT 501 GGCCGGGGCG GAAGCTGGTG GCGATGTTGC CGTAGTCTTT GCTGCGCTGG 551 TCGGTATTGC GGATTAA This corresponds to the amino acid sequence <SEQ ID 1002; ORF 255>: m255.pep 1 WGQEALRGQ FVAVFAAALR YAVKTCADFH AFDGVDAHHR VGDFGIEAVK 51 ΝRFAQADRDI GCFDMQLRAD GIQGFAHAVH IVFQLGΝLAM VGGKKRILGN 101 VFAAFKPDFF FADLGNVGGD FRAEFFFQPF FGNGSGSNAG GGFTGGAPAA 151 AAWARAVFV PIGIVGVAGA EAGGDVAWF AALVGIAD* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 255 shows 88.8% identity over a 188 aa overlap with a predicted ORF (ORF 255.ng) from N. gonorrhoeae: m255/g255 10 20 30 40 50 60 m255 . pep WGQEALRGQFVAVFAAALRYAVKTCADFHAFDGVDAHHRVGDFGIEAVKΝRFAQADRDI M M M M M M M M M M M M M M M M M I M M M M M M I h l I M M h g255 MVGQEALRGQFVAVFAAALRYAVKTCADFHAFDGVDAHHRVGDFGIEAVEΝGFAQTDGDV 10 20 30 40 50 60 70 80 90 100 110 120 m255.pep GCFDMQLRADGIQGFAHAVHIVFQLGΝLAMVGGKKRILGΝVFAAFKPDFFFADLGΝVGGD I g255 GGFDMQFRADGIQGFAHTVHIVFQFGDLALVGGKKRILGΝVFAAFKPDFFFADLGΝVGGD 70 80 90 100 110 120 130 140 150 160 170 180 m255 . pep FRAEFFFQPFFGΝGSGSΝAGGGFTGGAPAAAAWARAVFVPIGIVGVAGAEAGGDVAWF M M M M M M M M M M I h l h l l l l I I 1 1 = I I I 1 1 I I I I h 1 1 h 1 1 I I I I 1 1 g255 FRAEFFFQPFFGΝGSGGΝAGCGFAGGTPAAAPWARTVFVPIGIVGVSGAEGGGDVAWF 130 140 150 160 170 180 189 m255. pep AALVGIADX llllhlll g255 AALVGVADX The following partial DNA sequence was identified in N meningitidis <SEQ ID 1003>: a255. seq 1 GTGGTTGGAC AGGAAGCCTT GCGGGGTGAG TTCGTCGCCG TGTTCGCTGC 51 CGCGTTGCGT TACGCTGTCA AAACCTGCGC CGATTTCCAC GCCTTTGACG 101 GCGTTGATGC CCATCATGGC GTAGGCGATT TCGGCATCGA GGCGGTCGAA 151 TACGGGTTCG CCCAAGCCGA CGGGGACGTT GGCGGCTTCA ATATGCAGCT 201 TCGCGCCGAC GGAATCCAAG GATTTGCGCA CGCTGTCCAT ATAGTTTTCC 251 AGCTCGGCAA TTTGGCTATG GTTGGCGGCA AAAAAAGGAT TTTGGGAAAT 301 GTGTTCGCAG CCTTCAAACC GGATTTCTTT TTCGCCGACT TGGGTAACGT 351 AGGCGGTGAT TTCCGTGCCG AATTTTTCTT TCAACCATTT TTTGGCAACG 401 GCTCCGGCGG CAACGCGGGC GGCGGTTTCG CGGGCGGAAC TCCTGCCGCC 451 GCCCCGGTAG TCGCGCGTGC CGTATTTGTG CCAATAGGTA TAGTCGGCGT 501 GGCCGGGGCG GAAGCTGGTG GCGATGTTGC CGTAGTCTTT GCTGCGCTGG 551 TCGGTATTGC GGATTAA This corresponds to the amino acid sequence <SEQ ID 1004; ORF 255. a>: a255.pep 1 WGQEALRGE FVAVFAAALR YAVKTCADFH AFDGVDAHHG VGDFGIEAVE 51 YGFAQADGDV GGFΝMQLRAD GIQGFAHAVH IVFQLGΝLAM VGGKKRILGN 101 VFAAFKPDFF FADLGNVGGD FRAEFFFQPF FGNGSGGNAG GGFAGGTPAA 151 APWARAVFV PIGIVGVAGA EAGGDVAWF AALVGIAD* m255/a255 93.1% identity in 188 aa overlap 10 20 30 40 50 60 m255. pep WGQEALRGQFVAVFAAALRYAVKTCADFHAFDGVDAHHRVGDFGIEAVKNRFAQADRDI I I II II I II : I I II I II II I I I I I I II I II I II II I I I I lllllllll: I I I I I I : a255 WGQEALRGEFVAVFAAALRYAVKTCADFHAFDGVDAHHGVGDFGIEAVEYGFAQADGDV 10 20 30 40 50 60 70 80 90 100 110 120 m255.pep GCFDMQLRADGIQGFAHAVHIVFQLGNLAMVGGKKRILGNVFAAFKPDFFFADLGNVGGD I I : II I I II II II I I II II I II II I II I I II II I I II II II I I II I II II I I I II I II I a255 GGFNMQLRADGIQGFAHAVHIVFQLGNLAMVGGKKRILGNVFAAFKPDFFFADLGNVGGD 70 80 90 100 110 120 130 140 150 160 170 180 m255. pep FRAEFFFQPFFGNGSGSNAGGGFTGGAPAAAAWARAVFVPIGIVGVAGAEAGGDVAWF I II II II II I II II I I : I II II I : II : I II I II II II II II I I I II II II I II II II II a255 FRAEFFFQPFFGNGSGGNAGGGFAGGTPAAAPWARAVFVPIGIVGVAGAEAGGDVAWF 130 140 150 160 170 180 189 m255. pep AALVGIADX II II II I II a255 AALVGIADX The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1005>: g256. seq 1 atgctcgcgg tacgcaatcg gggttggcac ggcgcagtcg tccatttccg 51 cagctgcggc ggcgtagcga acaccgcccc ggtgttctac cacttgggtg 101 ataccgccga aatcgccttt gctttggaca cgctcaccgc gcgttaccgt 151 gaaatatacg ccgtcggcgt atcgctgggc ggcaacgcgc cggcaaaata 201 tttgggcgaa cagggcaaaa aggcattgcc gcacgcctcg gccgccgtat 251 ccgcccccgt tgatgcagag gcggcaggca gccgcttcga cagcggcatc 301 acgcggctgc tctacacgcg ctacttcctc cgcacactga tacccaaagc 351 acgttcgctc caaggttttc agacggcatt tgccgcaggg tgcaaaacac 401 tgggcgagtt tgacgaccgt ttcaccgcac cgctgcacgg ctttgccgac 451 cggcacgact actaccgcca aacttcctgc aaaccgctgc tcaaacacgt 501 tgccaaaccg ctgctcctgc tcaatgccgc caacgacccc ttcctgccgc 551 ccgaagccct gccccgtgca gacgaagcgt ccgaagccgt taccctgttc 601 caacctgcac acggcgggca cgccggcttt gtcagcagca ccggcggcag 651 gctgcacctg caatggctgc cgcagaccgt cctgtcctat tttgacagct 701 tccgcacaaa caggcgttaa This corresponds to the amino acid sequence <SEQ ID 1006; ORF 256.ng>: g256.pep 1 MLAVRNRGWH GA HFRSCG GVANTAPVFY HLGDTAEIAF ALDTLTARYR 51 EIYAVGVSLG GNAPAKYLGE QGKKALPHAS AAVSAPVDAE AAGSRFDSGI 101 TRLLYTRYFL RTLIPKARSL QGFQTAFAAG CKTLGEFDDR FTAPLHGFAD 151 RHDYYRQTSC KPLLKHVAKP LLLLNAANDP FLPPEALPRA DEASEAVTLF 201 QPAHGGHAGF VSSTGGRLHL QWLPQTVLSY FDSFRTNRR* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1007>: m256.seq 1 ATGCTTGCGG TACGCGATCG GGGTTGGCAC GGCGTAGTCG TCCATTTCCG 51 CAGCTGCGGC GGCATTGCCA ACACCGCTCC GGTGTTCTAC CA.CTtGGCG 101 ATACCGCCGA AATCGCCTTT ACTTTGGACA CGTTCGCCGC GCGTTACCGT 151 GAAAtATACG CCGTCGGCGT ATCGCTGGGC GGCAACGCGC TGGCAAAATA 201 TTTGGGCGAA CAGGGCAAAA AGGCATTGCC GCAAGCCGCT GCCGTCATCT 251 CCGCCCCCGT CGATGCAGAG GCGGCAGGCA GACGCTTCGA CAGCGGCATC 301 ACGCGGCTGC TCTACACGCG CTACTTCCTC CGCACCCTGA TACCCAAAGC 351 AAAATCGCTC CAAGGTTTTC AGACGGCATT TGCCGCAGGG TGCAAAACAC 401 TGGGCGAGTT TGACGACCGC TTCACCGCAC CGCTGCACGG CTTTGCCGAC 451 CGGCACGACT ACTACCGCCA AACTTCCTGC AAACCGCTGC TCAAACACGT 501 TGCCAAACCG CTGCTCCTGC TCAATGCCGT CAACGACCCC TTCCTGCCGC 551 CCGAAGCCCT GCCCCGCGCA GACGAAGTAT CCGAAGCCGT TACCCTGTTC 601 CAGCCGGCAT ATGGTGGTCA TGTCGGCTTT GTCAGCAGCA CCGGCGGCAG 651 GCTGCACCTG CAATGGCTGC CGCAGACCGT CCTGTCCTAT TTCGACAGCT 701 TCCGCACAAA CAGGCGTTAA This corresponds to the amino acid sequence <SEQ ID 1008; ORF 256>: m256.pep 1 MLAVRDRGWH GVWHFRSCG GIAΝTAPVFY XLGDTAEIAF TLDTFAARYR 51 EIYAVGVSLG GΝALAKYLGE QGKKALPQAA AVISAPVDAE AAGRRFDSGI 101 TRLLYTRYFL RTLIPKAKSL QGFQTAFAAG CKTLGEFDDR FTAPLHGFAD 151 RHDYYRQTSC KPLLKHVAKP LLLLΝAVΝDP FLPPEALPRA DEVSEAVTLF 201 QPAYGGHVGF VSSTGGRLHL QWLPQTVLSY FDSFRTNRR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 256 shows 92.9% identity over a 239 aa overlap with a predicted ORF (ORF 256.ng) from N. gonorrhoeae: m256/g256 10 20 30 40 50 60 m256.pep MLAVRDRGWHGVWHFRSCGGIAΝTAPVFYHLGDTAEIAFTLDTFAARYREIYAVGVSLG g256 MLAVRΝRGWHGAWHFRSCGGVAΝTAPVFYHLGDTAEIAFALDTLTARYREIYAVGVSLG 10 20 30 40 50 60 70 80 90 100 110 120 m256.pep GΝALAKYLGEQGKKALPQAAAVISAPVDAEAAGRRFDSGITRLLYTRYFLRTLIPKAKSL I I I M M M M M M h h h M M M I M M I I I I I I 1 1 1 I I 1 1 I I 1 1 I I I I I h I I g256 GΝAPAKYLGEQGKKALPHASAAVSAPVDAEAAGSRFDSGITRLLYTRYFLRTLIPKARSL 70 80 90 100 110 120 130 140 150 160 170 180 m256.pep QGFQTAFAAGCKTLGEFDDRFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLΝAVΝDP M M M M M M M M M M M M M M M M M M M M M M M M M M M I h l l l g256 QGFQTAFAAGCKTLGEFDDRFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLΝAAΝDP 130 140 150 160 170 180 190 200 210 220 230 240 m256.pep FLPPEALPRADEVSEAVTLFQPAYGGHVGFVSSTGGRLHLQWLPQTVLSYFDSFRTNRRX M l l l l l I I I I I M I l l l l l l l h 1 1 h I I I I I M I I I I I 1 1 I I I M M M M I M M M g256 FLPPEALPRADEASEAVTLFQPAHGGHAGFVSSTGGRLHLQWLPQTVLSYFDSFRTNRRX 190 200 210 220 230 The following partial DNA sequence was identified inN meningitidis <SEQ ID 1009>: a256. seq 1 ATGCTCGCGG TACGCGATCG GGGTTGGAAC GGCGTAGTCG TCCATTTCCG 51 CAGCTGCGGC GGCGTAGCGA ACACCGCCCC GGTGTTCTAC CACTTGGGCG 101 ATACCGCCGA AATTGCCTTT ACTTTGGACA CGCTCGCCGC GCGTTACCGT 151 GAAATATACG CCGTCGGCGT ATCGCTGGGC GGCAACGCGC TGGCAAAATA 201 TTTGGGCGAA CAGGGCGAAA ACGCGCTGCC GCAAGCCGCC GCCGTCATCT 251 CCGCACCCGT CGATGCAGAG GCGGCAGGCA ACCGCTTCGA CAGCGGCATC 301 ACACGGCTGC TCTACACGCG CTACTTCCTC CGCACACTGA TACCCAAAGC 351 ACGGTCGCTC CAAGGTTTTC AGACGGCATT TGCCGCAGGG TGCAAAACAC 401 TGGGCGAGTT TGACGACCGT TTCACCGCAC CGCTGCACGG CTTTGCCGAT 451 CGGCACGACT ACTACCGCCA AACTTCCTGC AAACCGCTGC TCAAACACGT 501 TGCCAAACCG CTGCTCCTGC TCAATGCCGT CAACGACCCC TTCCTGCCGC 551 CCGAAGCGCT GCCCCGCGCA GACGAAGTGT CCGAAGCCGT TACCCTGTTC 601 CAGCCGACAC ACGGTGGTCA TGTCGGCTTT GTCGGCAGCA CCGGCGGCAG 651 GCTGCACCTG CAATGGTTGC CGCAGACCGT CCTGTCCTAT TTCGACAGCT 701 TCCGCACAAA CAGGCGTTAA This corresponds to the amino acid sequence <SEQ ID 1010; ORF 256.a>: a256.pep 1 MLAVRDRGWΝ GVWHFRSCG GVANTAPVFY HLGDTAEIAF TLDTLAARYR 51 EIYAVGVSLG GNALAKYLGE QGENALPQAA AVISAPVDAE AAGNRFDSGI 101 TRLLYTRYFL RTLIPKARSL QGFQTAFAAG CKTLGEFDDR FTAPLHGFAD 151 RHDYYRQTSC KPLLKHVAKP LLLLNAVNDP FLPPEALPRA DEVSEAVTLF 201 QPTHGGHVGF VGSTGGRLHL QWLPQTVLSY FDSFRTNRR* m256/a256 95.4% identity in 239 aa overlap 10 20 30 40 50 60 m256. pep MLAVRDRGWHGVWHFRSCGGIANTAPVFYXLGDTAEIAFTLDTFAARYREIYAVGVSLG I II II II II: II I I II I II II : II I I II I I I II II I II II II I : II I II II I II II II I a256 MLAVRDRGWNGVWHFRSCGGVANTAPVFYHLGDTAEIAFTLDTLAARYREIYAVGVSLG 10 20 30 40 50 60 70 80 90 100 110 120 m256. pep GNALAKYLGEQGKKALPQAAAVISAPVDAEAAGRRFDSGITRLLYTRYFLRTLIPKAKSL I II II II II I II :: II I II I I I II I I II I I I I I II I II II II II II II II II I II I : II a256 GNALAKYLGEQGENALPQAAAVISAPVDAEAAGNRFDSGITRLLYTRYFLRTLIPKARSL 70 80 90 100 110 120 130 140 150 160 170 180 m256. pep QGFQTAFAAGCKTLGEFDDRFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAVNDP I II II II II I II I II I II I I I I II I I I II II I II II I II II I II II I II II I II II I II I a256 QGFQTAFAAGCKTLGEFDDRFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAVNDP 130 140 150 160 170 180 190 200 210 220 230 240 m256. pep FLPPEALPRADEVSEAVTLFQPAYGGHVGFVSSTGGRLHLQWLPQTVLSYFDSFRTNRRX II I I I I II II II I II II I II I I :: II I I I I I : I I I I II I II I II II II II II I II II I I I a256 FLPPEALPRADEVSEAVTLFQPTHGGHVGFVGSTGGRLHLQWLPQTVLSYFDSFRTNRRX 190 200 210 220 230 240 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ED 1011>: g256-l.seq 1 ATGATTTTGA CACCGCCGGA CACGCCCTTT TTCCTCCGCA ACGGCAATGC 51 CGACACGATT GCCGCCAAAT TCCTGCAACA CCCCGCACCC GCATACCGCC 101 GCGAGATGCT TCCCGACAGC ACGGGTAAAA CCAAAACCGC CTACGACTTT 151 TCAGCAGGCG GCATTTCGCC CGATGCGCCG CTGGTCGTGC TGTTTCACGG 201 TTTGGAAGGA AGCAGCCGCA GCCATTACGC GGTCGAACTG ATGCTCGCGG 251 TACGCAATCG GGGTTGGCAC GGCGCAGTCG TCCATTTCCG CAGCTGCGGC 301 GGCGTAGCGA ACACCGCCCC GGTGTTCTAC CACTTGGGTG ATACCGCCGA 351 AATCGCCTTT GCTTTGGACA CGCTCACCGC GCGTTACCGT GAAATATACG 401 CCGTCGGCGT ATCGCTGGGC GGCAACGCGC CGGCAAAATA TTTGGGCGAA 451 CAGGGCAAAA AGGCATTGCC GCACGCCTCG GCCGCCGTAT CCGCCCCCGT 501 TGATGCAGAG GCGGCAGGCA GCCGCTTCGA CAGCGGCATC ACGCGGCTGC 551 TCTACACGCG CTACTTCCTC CGCACACTGA TACCCAAAGC ACGTTCGCTC 601 CAAGGTTTTC AGACGGCATT TGCCGCAGGG TGCAAAACAC TGGGCGAGTT 651 TGACGACCGT TTCACCGCAC CGCTGCACGG CTTTGCCGAC CGGCACGACT 701 ACTACCGCCA AACTTCCTGC AAACCGCTGC TCAAACACGT TGCCAAACCG 751 CTGCTCCTGC TCAATGCCGC CAACGACCCC TTCCTGCCGC CCGAAGCCCT 801 GCCCCGTGCA GACGAAGCGT CCGAAGCCGT TACCCTGTTC CAACCTGCAC 851 ACGGCGGGCA CGCCGGCTTT GTCAGCAGCA CCGGCGGCAG GCTGCACCTG 901 CAATGGCTGC CGCAGACCGT CCTGTCCTAT TTTGACAGCT TCCGCACAAA 951 CAGGCGTTAA This corresponds to the amino acid sequence <SEQ ID 1012; ORF 256-l.ng>: g256-l.pep 1 MILTPPDTPF FLR GNADTI AAKFLQHPAP AYRREMLPDS TGKTKTAYDF 51 ΞAGGISPDAP LVVLFHGLEG SSRSHYAVEL MLAVRNRGWH GAWHFRSCG 101 GVANTAPVFY HLGDTAEIAF ALDTLTARYR EIYAVGVSLG GNAPAKYLGE 151 QGKKALPHAS AAVSAPVDAE AAGSRFDSGI TRLLYTRYFL RTLIPKARSL 201 QGFQTAFAAG CKTLGEFDDR FTAPLHGFAD RHDYYRQTSC KPLLKHVAKP 251 LLLLNAANDP FLPPEALPRA DEASEAVTLF QPAHGGHAGF VSSTGGRLHL 301 QWLPQTVLSY FDSFRTNRR* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1013>: m256-l. seq 1 ATGATTTTAA CACCGCCGGA CACGCCCTTT TTCCTCCGCA ACGGCAATGC 51 CGACACGATT GCCGCCAAAT TCCTGCAACG CCCCGCGCCC GCATACCGCC 101 GAGAGCTGCT TCCCGACAGC ACGGGTAAAA CCAAAGTCGC CTACGACTTT 151 TCAGACGGCA TTTCGCCCGA TGCGCCGCTG GTCGTGCTGT TTCACGGTTT 201 GGAAGGAAGC AGCCGCAGCC ATTACGCGGT CGAACTGATG CTTGCGGTAC 251 GCGATCGGGG TTGGCACGGC GTAGTCGTCC ATTTCCGCAG CTGCGGCGGC 301 ATTGCCAACA CCGCTCCGGT GTTCTACCAC TTGGGCGATA CCGCCGAAAT 351 CGCCTTTACT TTGGACACGT TCGCCGCGCG TTACCGTGAA ATATACGCCG 401 TCGGCGTATC GCTGGGCGGC AACGCGCTGG CAAAATATTT GGGCGAACAG 451 GGCAAAAAGG CATTGCCGCA AGCCGCTGCC GTCATCTCCG CCCCCGTCGA 501 TGCAGAGGCG GCAGGCAGAC GCTTCGACAG CGGCATCACG CGGCTGCTCT 551 ACACGCGCTA CTTCCTCCGC ACCCTGATAC CCAAAGCAAA ATCGCTCCAA 601 GGTTTTCAGA CGGCATTTGC CGCAGGGTGC AAAACACTGG GCGAGTTTGA 651 CGACCGCTTC ACCGCACCGC TGCACGGCTT TGCCGACCGG CACGACTACT 701 ACCGCCAAAC TTCCTGCAAA CCGCTGCTCA AACACGTTGC CAAACCGCTG 751 CTCCTGCTCA ATGCCGTCAA CGACCCCTTC CTGCCGCCCG AAGCCCTGCC 801 CCGCGCAGAC GAAGTATCCG AAGCCGTTAC CCTGTTCCAG CCGGCATATG 851 GTGGTCATGT CGGCTTTGTC AGCAGCACCG GCGGCAGGCT GCACCTGCAA 901 TGGCTGCCGC AGACCGTCCT GTCCTATTTC GACAGCTTCC GCACAAACAG 951 GCGTTAA This corresponds to the amino acid sequence <SEQ ID 1014; ORF 256-l>: m256-l.pep 1 MILTPPDTPF FLR GΝADTI AAKFLQRPAP AYRRELLPDS TGKTKVAYDF 51 SDGISPDAPL VVLFHGLEGS SRSHYAVELM LAVRDRGWHG VVVHFRSCGG 101 IAΝTAPVFYH LGDTAEIAFT LDTFAARYRE IYAVGVSLGG ΝALAKYLGEQ 151 GKKALPQAAA VISAPVDAEA AGRRFDSGIT RLLYTRYFLR TLIPKAKSLQ 201 GFQTAFAAGC KTLGEFDDRF TAPLHGFADR HDYYRQTSCK PLLKHVAKPL 251 LLLΝAVΝDPF LPPEALPRAD EVSEAVTLFQ PAYGGHVGFV SΞTGGRLHLQ 301 WLPQTVLSYF DSFRTΝRR* m256-l/g256-l 93.1% identity in 319 aa overlap 10 20 30 40 50 59 m256-l . pep MILTPPDTPFFLRΝGΝADTIAAKFLQRPAPAYRRELLPDSTGKTKVAYDFS-DGISPDAP I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I :l I I I I I I I hi I I I I lllllll g256-l MILTPPDTPFFLRΝGΝADTIAAKFLQHPAPAYRREMLPDSTGKTKTAYDFΞAGGISPDAP 10 20 30 40 50 60 60 70 80 90 100 110 119 m256-l . pep LVVLFHGLEGSSRSHYAVELMLAVRDRGWHGVVVHFRSCGGIAΝTAPVFYHLGDTAEIAF I I I I I I I I I I I I I II I I I I II III hi I I I hi I I II I I I hi I I I I I I I I I I I I I I I I I g256-l LVVLFHGLEGSSRSHYAVELMLAVRNRGWHGAVVHFRΞCGGVANTAPVFYHLGDTAEIAF 70 80 90 100 110 120 120 130 140 150 160 170 179 m256-l.pep TLDTFAARYREIYAVGVSLGGNALAKYLGEQGKKALPQAAAVISAPVDAEAAGRRFDSGI : I I I :: I I I I I I I I I I I I I I I 1 I I I II I I I I I I I I I : h I : : I I I I I I I I I I g256-l ALDTLTARYREIYAVGVSLGGNAPAKYLGEQGKKALPHASAAVSAPVDAEAAGΞRFDSGI 130 140 150 160 170 180 180 190 200 210 220 230 239 m256-l.pep TRLLYTRYFLRTLIPKAKSLQGFQTAFAAGCKTLGEFDDRFTAPLHGFADRHDYYRQTSC I I I I I I I I I I I II I I I hi I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g256-l TRLLYTRYFLRTLIPKARSLQGFQTAFAAGCKTLGEFDDRFTAPLHGFADRHDYYRQTSC 190 200 210 220 230 240 240 250 260 270 280 290 299 Itl256-l.pep KPLLKHVAKPLLLLNAVNDPFLPPEALPRADEVSEAVTLFQPAYGGHVGFVSSTGGRLHL I I I I I I I I I I I I I I I h I I I I I I I I I I I I I I hi I I I I I I I I I :| I I : I I I I I I I I I I I I g256-l KPLLKHVAKPLLLLNAANDPFLPPEALPRADEASEAVTLFQPAHGGHAGFVΞSTGGRLHL 250 260 270 280 290 300 300 310 319 m256-l.pep QWLPQTVLSYFDSFRTNRRX I I I I I I I I I I I I I I I I I I I I g256-l QWLPQTVLSYFDSFRTNRRX 310 320 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1015>: a256-l.seq 1 ATGATTTTGA CACCGCCGGA CACACCCTTT TTCCTCCGCA ACGGCAATGC 51 CGACACGATT GCCGCCAAAT TCCTGCAACG CTCCGCACCT GCATACCGCC 101 GCGAGCTGCT TCCCGACAGC ACGGGTAAAA CCAAAACCGC CTACGACTTT 151 TCAGACGGCA TTTCGCCCGA TGCGCCGCTG GTCGTGCTGT TTCACGGTTT 201 GGAGGGCGGC AGTGGCAGCC ATTACGCGGT CGAACTGATG CTCGCGGTAC 251 GCGATCGGGG TTGGAACGGC GTAGTCGTCC ATTTCCGCAG CTGCGGCGGC 301 GTAGCGAACA CCGCCCCGGT GTTCTACCAC TTGGGCGATA CCGCCGAAAT 351 TGCCTTTACT TTGGACACGC TCGCCGCGCG TTACCGTGAA ATATACGCCG 401 TCGGCGTATC GCTGGGCGGC AACGCGCTGG CAAAATATTT GGGCGAACAG 451 GGCGAAAACG CGCTGCCGCA AGCCGCCGCC GTCATCTCCG CACCCGTCGA 501 TGCAGAGGCG GCAGGCAACC GCTTCGACAG CGGCATCACA CGGCTGCTCT 551 ACACGCGCTA CTTCCTCCGC ACACTGATAC CCAAAGCACG GTCGCTCCAA 601 GGTTTTCAGA CGGCATTTGC CGCAGGGTGC AAAACACTGG GCGAGTTTGA 651 CGACCGTTTC ACCGCACCGC TGCACGGCTT TGCCGATCGG CACGACTACT 701 ACCGCCAAAC TTCCTGCAAA CCGCTGCTCA AACACGTTGC CAAACCGCTG 751 CTCCTGCTCA ATGCCGTCAA CGACCCCTTC CTGCCGCCCG AAGCGCTGCC 801 CCGCGCAGAC GAAGTGTCCG AAGCCGTTAC CCTGTTCCAG CCGACACACG 851 GTGGTCATGT CGGCTTTGTC GGCAGCACCG GCGGCAGGCT GCACCTGCAA 901 TGGTTGCCGC AGACCGTCCT GTCCTATTTC GACAGCTTCC GCACAAACAG 951 GCGTTAA This corresponds to the amino acid sequence <SEQ ID 1016; ORF 256-1. a>: a256-l.pep 1 MILTPPDTPF FLRΝGΝADTI AAKFLQRSAP AYRRELLPDS TGKTKTAYDF 51 SDGISPDAPL VVLFHGLEGG SGSHYAVELM LAVRDRGWΝG VVVHFRSCGG 101 VAΝTAPVFYH LGDTAEIAFT LDTLAARYRE IYAVGVSLGG ΝALAKYLGEQ 151 GEΝALPQAAA VISAPVDAEA AGΝRFDSGIT RLLYTRYFLR TLIPKARΞLQ 201 GFQTAFAAGC KTLGEFDDRF TAPLHGFADR HDYYRQTSCK PLLKHVAKPL 251 LLLΝAVΝDPF LPPEALPRAD EVSEAVTLFQ PTHGGHVGFV GSTGGRLHLQ 301 WLPQTVLSYF DSFRTΝRR* a256-l/ 256-l 95.6% identity in 318 aa overlap 10 20 30 40 50 60 a256-l.pep MILTPPDTPFFLRΝGΝADTIAAKFLQRSAPAYRRELLPDSTGKTKTAYDFΞDGISPDAPL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I h I I I I I I I I I I I I I I m256-l MILTPPDTPFFLRΝGΝADTIAAKFLQRPAPAYRRELLPDSTGKTKVAYDFSDGISPDAPL 10 20 30 40 50 60 70 80 90 100 110 120 a256-1.pep VVLFHGLEGGSGSHYAVELMLAVRDRGWΝGVVVHFRSCGGVAΝTAPVFYHLGDTAEIAFT I I I I I I I I h I I I I I I I I I I I I I I I I hi I I I I I I I I I hi I I I I I I I I I I I I I I I I I I πι256-l VVLFHGLEGSSRSHYAVELMLAVRDRGWHGVVVHFRSCGGIAΝTAPVFYHLGDTAEIAFT 70 90 100 110 120 130 140 150 160 170 180 a256-1. pep LDTLAARYREIYAVGVSLGGNALAKYLGEQGENALPQAAAVISAPVDAEAAGNRFDSGIT I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I ::| I I II I I I I I I I I I I I I I I lllllll m256-l LDTFAARYREIYAVGVΞLGGNALAKYLGEQGKKALPQAAAVISAPVDAEAAGRRFDΞGIT 130 140 150 160 170 180 190 200 210 220 230 240 a256-l.pep RLLYTRYFLRTLIPKARSLQGFQTAFAAGCKTLGEFDDRFTAPLHGFADRHDYYRQTSCK I I I I I I I I I I I I I I I hi I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I m256-l RLLYTRYFLRTLIPKAKSLQGFQTAFAAGCKTLGEFDDRFTAPLHGFADRHDYYRQTSCK 190 200 210 220 230 240 250 260 270 280 290 300 a256-l.pep PLLKHVAKPLLLLNAVNDPFLPPEALPRADEVSEAVTLFQPTHGGHVGFVGSTGGRLHLQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ::| I I I I I hi I I I I I I I I m256-l PLLKHVAKPLLLLNAVNDPFLPPEALPRADEVSEAVTLFQPAYGGHVGFVΞSTGGRLHLQ 250 260 270 280 290 300 310 319 a256-l.pep WLPQTVLSYFDSFRTNRRX I I I I I I I I I I I I I I I I I I I m256-l WLPQTVLSYFDSFRTNRRX 310 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1017>: g257.seq 1 atgggcaggc atttcgggcg cagacgtttt ctgacggctg ccgccgttgc 51 tgtggccggt gcggcggttt cttttttgcc gaatcctttt gccgccggcg 101 gcgaaaaacg caacatggat aaaaaacgcg atgaaaatgt gtttttctgg 151 aaaggtgtcg cgctgggttc cggcgcggag ctgcgcctgt tcggcgtgga 201 cgacagacag gcggcggatt tggtcaataa ggttttggcg gaagtggcgc 251 gtttggaaaa aatgttcagc ctttaccgtg aagacagcct gatcagccgt 301 ctgaaccgcg acggttatct gacttcgcct ccggcggatt ttttggaact 351 gttgagcctg gccgcgatat tcacgcgctg a This corresponds to the amino acid sequence <SEQ ID 1018; ORF 257.ng>: g257.peρ 1 MGRHFGRRRF LTAAAVAVAG AAVSFLPΝPF AAGGEKRΝMD KKRDEΝVFFW 51 KGVALGSGAE LRLFGVDDRQ AADLVΝKVLA EVARLEKMFS LYREDSLISR 101 LΝRDGYLTSP PADFLELLSL AAIFTR* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1019>: m257. seq 1 ATGGGCAGGC ATTTCGGGCG . CAGCGTTTT CTGACGGTTG CCGCCGTTGC 51 GGCGGGGaC. GCGGcGGTTT CTTTCCTGCC GAATCCTTTT GCCGCCGATG 101 ATGAAAAACG CAAcGGGGAT GAAAAACGCA ATGAAAATGT GTTTTTCTGG 151 AAAGGTGTCG CACTGGGTTC CGGTGCGGa . CTCCGTCTGT TCGGTGTGGA 201 CGACAGGCGT GCGGCGGATT TGGTCAACAA GGTTTTGGCG GAAGTGGCGC 251 GTTTGGAAAA ATTGTTCAGC CTTTACCGTG AAGACAGCCT GATCAGCCGC 301 CTGAACAGGG ACGGTTATCT GACTTCGCCG TCGGCGGATT TTTTGGAACT 351 G TGAGCCTG GCCGCGATAT TCACGCkCTG A This corresponds to the amino acid sequence <SEQ ID 1020; ORF 257>: m257.pep 1 MGRHFGXQRF LTVAAVAAGX AAVSFLPΝPF AADDEKRΝGD EKRΝEΝVFFW 51 KGVALGSGAX LRLFGVDDRR AADLVΝKVLA EVARLEKLFS LYREDSLISR 101 LΝRDGYLTSP SADFLELXSL AAIFTX* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 257 shows 88.0% identity over a 125 aa overlap with a predicted ORF (ORF 257.ng) from N gonorrhoeae: m257/g257 10 20 30 40 50 60 m257 . pep MGRHFGRQRFLTVAAVAAGTAAVSFLPNPFAADDEKRNGDEKRNENVFFWKGVALGSGAD I I M M I M I I I M I I h : M M h I h I I I I I I I I I I 1 1 1 1 h g257 MGRHFGRRRFLTAAAVAVAGAAVSFLPNPFAAGGEKRNMDKKRDENVFFWKGVALGSGAE 10 20 30 40 50 60 70 80 90 100 110 120 m257. pep LRLFGVDDRRAADLVNKVLAEVARLEKLFSLYREDSLISRLNRDGYLTSPSADFLELXSL MMMMhMMMMMMMMhMMMMMMMMMMM M g257 LRLFGVDDRQAADLVNKVLAEVARLEKMFSLYREDSLISRLNRDGYLTSPPADFLELLSL 70 80 90 100 110 120 m257.pep AAIFTXX I II II I g257 AAIFTRX The following partial DNA sequence was identified in N meningitidis <SEQ LD 1021>: a257. seq 1 ATGGGCAGGC ATTTCGGGCG CAGGCGTTTT TTGACAGTTG CCGCCGTTGC 51 GGCGGCGGGC GCGGCGGTTT CTTTCCTGCC GAATCCTTTT GCCGCCGATG 101 ATGAAAAACG CAATAAAGAT GAAAAACGCA ATGAAAATGT GTTTTTCTGG 151 AAAGGTGTCG CACTGGGTTC CGGTGCGGAG CTCCGTCTGT TCGGTGTGGA 201 CGACAGGCGT GCGGCGGATT TGGTCAACAA GGTTTTGGCG GAAGTGGCGC 251 GTTTGGAAAA AATGTTCAGC CTTTACCGTG AAGACAGCCT GATCAGCCGT 301 CTGAACCGTG ACGGTTATTT GACTTCGCCG CCGGCGGATT TTTTGGAACT 351 GTTGAGCCTG GCCGTGATAT TCACGCGCTG A This corresponds to the amino acid sequence <SEQ ID 1022; ORF 257.a>: a257.pep 1 MGRHFGRRRF LTVAAVAAAG AAVSFLPΝPF AADDEKRΝKD EKRΝEΝVFFW 51 KGVALGSGAE LRLFGVDDRR AADLVΝKVLA EVARLEKMFS LYREDSLISR 101 LΝRDGYLTSP PADFLELLSL AVIFTR* m257/a257 92.0% identity in 125 aa overlap 10 20 30 40 50 60 m257.pep MGRHFGXQRFLTVAAVAAGXAAVSFLPΝPFAADDEKRΝGDEKRΝEΝVFFWKGVALGSGAX llllll : I I I II I I I I I : I I I II I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a257 MGRHFGRRRFLTVAAVAAAGAAVSFLPΝPFAADDEKRΝKDEKRΝEΝVFFWKGVALGSGAE 10 20 30 40 50 60 70 80 90 100 110 120 m257.pep LRLFGVDDRRAADLVΝKVLAEVARLEKLFSLYREDSLISRLΝRDGYLTSPSADFLELXSL I I I I I I I I I I I I I I I I I I I II I I I I I I : I I I I I I I I I I I I I I I I I I I I II llllll I I a257 LRLFGVDDRRAADLVΝKVLAEVARLEKMFSLYREDSLISRLΝRDGYLTSPPADFLELLSL 70 80 90 100 110 120 m257.pep AAIFTXX 1:111 a257 AVIFTRX The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1023>: g258.seq 1 atgcgccgct tcctaccgat cgcagccata tgcgccgtcg tcctgctgta 51 cggattgacg gcggcgaccg gcagcaccag ttcgctggcg gattatttct 101 ggtggatagt ctcgttcagc gcaatgctgc tgctggtgtt gtccgccgtt 151 ttggcacgtt atgtcatatt gctgttgaaa gacaggcgca acggcgtgtt 201 cggttcgcag attgccaaac gcctttccgg gatgttcacg ctggtcgccg 251 tactgcccgg cttgttcctg ttcggcattt ccgcgcagtt tatcaacggc 301 acgattaatt cgtggttcgg caacgacacc cacgaagccc tcgaacgcag 351 ccttaatttg agcaagtccg cactggattt ggcggcagac aatgccgtca 401 gcaacgccgt tcccgtacag atagacctca tcggcaccgc ctccctgtcg 451 ggcaatatgg gcagtgtgct ggaacactac gccggcagcg gttttgccca 501 gcttgccctg tacaatgccg caagcgggaa aatcgaaaaa agcatcaatc 551 cgcaccaatt cgaccagccg cttcccgaca aagaacattg ggaacagatt 601 cagcagaccg gttcggttcg gagtttggaa agcataggcg gcgtattgta 651 cgcgcaggga tggttgtcgg caggtacgca caacgggcgc gattacgcgc 701 tgttcttccg ccagccgatt cccgaaaatg tggcacagga tgccgttctg 751 attgaaaagg cgcgggcgaa atatgccgaa ttgagttaca gcaaaaaagg 801 tttgcagacc ttttttctgg taaccctgct gattgcctcg ctgctgtcga 851 tttttcttgc gctggtaatg gcactgtatt ttgcccgccg tttcgtcgaa 901 cccattctgt cgcttgccga gggcgcaaag gcggtggcgc agggtgattt 951 cagccagacg cgccccgtat tgcgcaacga cgagttcgga cgtttgacca 1001 agctgttcaa ccatatgacc gagcagcttt ccatcgccaa agaagcagac 1051 gaacgcaacc gccggcgcga ggaagccgcc cgtcactacc tcgagtgcgt 1101 gttggatggg ttgactaccg gtgtggtggt ctcntacccc ctctcttgtt 1151 gccgtaccgc ggtgttttcc acttgtcatt cctcccctct ttcttatttc 1201 taa This corresponds to the amino acid sequence <SEQ ID 1024; ORF 258.ng>: g258.pep 1 MRRFLPIAAI CAWLLYGLT AATGSTSSLA DYFWWIVSFS AMLLLVLSAV 51 LARYVILLLK DRRΝGVFGSQ IAKRLSGMFT LVAVLPGLFL FGISAQFIΝG 101 TIΝSWFGΝDT HEALERSLΝL SKSALDLAAD ΝAVSΝAVPVQ IDLIGTASLS 151 GΝMGSVLEHY AGSGFAQLAL YΝAASGKIEK SIΝPHQFDQP LPDKEHWEQI 201 QQTGSVRSLE SIGGVLYAQG WLSAGTHΝGR DYALFFRQPI PEΝVAQDAVL 251 IEKARAKYAE LSYSKKGLQT FFLVTLLIAS LLSIFLALVM ALYFARRFVE 301 PILSLAEGAK AVAQGDFSQT RPVLRΝDEFG RLTKLFΝHMT EQLSIAKEAD 351 ERΝRRREEAA RHYLECVLDG LTTGWVSYP LSCCRTAVFS TCHSSPLSYF* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1025>: m258. seq 1 ATGCGCCGTT TTCTACCGAT CGCAGCCATA TGCGCCGTCG TCCTGTTGTA 51 CGGACTGACG GCGGCAACCG GCAGCACCAG TTCGCTGGCG GATTATTTCT 101 GGTGGATTGT TGCGTTCAGC GCAATGCTGC TGCTGGTGTT GTCCGCCGTT 151 TTGGCACGTT ATGTCATATT GCTGTTGAAA GACAGGCGCG ACGGCGTATT 201 CGGTTCGCAG ATTGCCAAAC GCCTTTCTGG GATGTTTACG CTGGTTGCCG 251 TACTGCCCGG CGTGTTTCTG TTCGGCGTTT CCGCACAGTT CATCAACGGC 301 ACGATTAATT CGTGGTTCGG CAACGATACC CACGAGGCGC TTGAACGCAG 351 CCTCAATTTG AGCAAGTCCG CATTGAATTT GGCGGCAGAC AACGCCCTCG 401 GCAACGCCGT CCCCGTGCAG ATAGACCTCA TCGGCGCGGC TTCCCTGCCC 451 GGGGATATGG GCAGGGTGCT GGAACATTAC GCCGGCAGCG GTTTTGCCCA 501 GCTTGCCCTG TACAATGCCG CAAGCGGCAA AATCGAAAAA AGCATCAACC 551 CGCACAAGCT CGATCAGCCG TTTCCAGGTA AGGCGCGTTG GGAAAAAATC 601 CAACGGGCGG GTTCGGTCAG GGATTTGGAA AGCATAGGCG GCGTATTGTA 651 CGCGCAGGGC TGGCTGTCGG CGGGTACGCA CAACGGGCGC GATTACGCCT 701 TGTTTTTCCG TCAGCCGGTT CCCAAAGGCG TGGCAGAGGA TGCCGTCTTA 751 ATCGAAAAGG CAAGGGCGAA ATATGCTGAG TTGAGTTACA GCAAAAAAGG 801 TTTGCAGACC TTTTTCCTGG CAACCCTGCT GATTGCCTCG CTGCTGTCGA 851 TTTTTCTTGC ACTGGTCATG GCACTGTATT TCGCCCGCCG TTTCGTCGAA 901 CCCGTCCTAT CGCTTGCCGA GGGGGCGAAG GCGGTGGCGC AAGGCGATTT 951 CAGCCAGACG CGCCCCGTGT TGCGCAACGA CGAGTTCGGA CGCTTGACCA 1001 AGTTGTTCAA CCACATGACC GAGCAGCTTT CCATCGCCAA AGAAGCAGAC 1051 GAGCGCAACC GCCGGCGCGA GGAAGCCGCC AGGCATTATC TTGAATGCGT 1101 GTTGGAGGGG CTGACCACGG GCGTGGTGGT GTTTGACGAA CAAGGCTGTC 1151 TGAAAACsTT CAACAAAGCG GCGGAACAGA TTyTGGGGAT GCCGCTTACC 1201 CCCcTGTGGG GCAGCAGCCG GCACGGTTGG CACGGCGTTT CGGCGCAGCA 1251 GTCCCTGCTT GCCGAAGTGT TTGCCGCCAT CGGCGCGGCG GCAGGTACGG 1301 ACAAACCGGT CCATGTGAAA TATGCCGCGC CGGACGATGC CAAAATCCTG 1351 CTGGGCAAGG CAACCGTCCT GCCCGAAGAC AACGGCAACg GCGTGGTAAT 1401 GGTGATTGAC GACATCACCG TTTTGATACA CGCGCAAAAA GAAGCCGCGT 1451 GGGGCGAAgT GGCGaAgCGG CTGGCACACG AAATCCGCAA TCCGCTCACG 1501 CCCATCCAGC TTTCCGCCGA ACgGsTGGCG TkGAAATTGG GCGGGAAGCT 1551 GGATGAGCAG GATGCGCAAA TCCTGACGCG TTCGACCGAC ACCATCGTCA 1601 AACAGGTGGC GGCATTGAAG GAAATGGTCG AAGCATTCCG CAATTATGCG 1651 CGTTCCCCTT CGCTCAAATT GGAAAATCAG GATTTGAACG CCTTAATCGG 1701 CGATGTGTTG GCATTGTATG AAGCCGGTCC GTGCCGGTTT GCGGCGGACT 1751 TGCCGGCGAA CCGCTGA This corresponds to the amino acid sequence <SEQ ED 1026; ORF 258>: m258.pep 1 MRRFLPIAAI CAWLLYGLT AATGSTSSLA DYFWWIVAFS AMLLLVLSAV 51 LARYVILLLK DRRDGVFGSQ IAKRLSGMFT LVAVLPGVFL FGVSAQFING 101 TINSWFGNDT HEALERSLNL SKSALNLAAD NALGNAVPVQ IDLIGAASLP 151 GDMGRVLEHY AGSGFAQLAL YNAASGKIEK SINPHKLDQP FPGKARWEKI 201 QRAGSVRDLE SIGGVLYAQG WLSAGTHNGR DYALFFRQPV PKGVAEDAVL 251 IEKARAKYAE LSYSKKGLQT FFLATLLIAS LLSIFLALVM ALYFARRFVE 301 PVLSLAEGAK AVAQGDFSQT RPVLRNDEFG RLTKLFNHMT EQLSIAKEAD 351 ERNRRREEAA RHYLECVLEG LTTGVWFDE QGCLKTFNKA AEQILGMPLT 401 PLWGSSRHGW HGVSAQQSLL AEVFAAIGAA AGTDKPVHVK YAAPDDAKIL 451 LGKATVLPED NGNGWMVID DITVLIHAQK EAAWGEVAKR LAHEIRNPLT 501 PIQLSAERXA XKLGGKLDEQ DAQILTRSTD TIVKQVAALK EMVEAFRNYA 551 RSPSLKLENQ DLNALIGDVL ALYEAGPCRF AADLPANR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 258 shows 90.9% identity over a 386 aa overlap with a predicted ORF (ORF 258.ng) from N gonorrhoeae: m258/g258 10 20 30 40 50 60 m258.pep MRRFLPIAAICAWLLYGLTAATGSTSSLADYFWWIVAFSAMLLLVLSAVLARYVILLLK M M I M M i ! M I M M M M M M M M I M M I I M M M M g258 MRRFLPIAAI CAWLLYGLTAATGSTSSLADYFWWIVSFSAMLLLVLSAVLARYVILLLK 10 20 30 40 50 60 70 80 90 100 110 120 m258. pep DRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFIΝGTIΝSWFGΝDTHEALERSLΝL MhMMMMMMMMMMMhMlhMMMMMMMMMMMMIM g258 DRRΝGVFGSQIAKRLSGMFTLVAVLPGLFLFGISAQFIΝGTIΝSWFGΝDTHEALERSLΝL 70 80 90 100 110 120 130 140 150 160 170 180 m258. pep SKSALΝLAADΝALGΝAVPVQIDLIGAASLPGDMGRVLEHYAGSGFAQLALYΝAASGKIEK I I I I I M I I I I h M l l l l l l l l l h l l l M 1 1 I I 1 1 I I 1 1 I I 1 1 I I I I I I I I I I I g258 SKSALDLAADΝAVSΝAVPVQIDLIGTASLSGΝMGSVLEHYAGSGFAQLALYΝAASGKIEK 130 140 150 160 170 180 190 200 210 220 230 240 m258.pep SIΝPHKLDQPFPGKARWEKIQRAGSVRDLESIGGVLYAQGWLSAGTHΝGRDYALFFRQPV 11 II I = : 111 : 1 I : I h I h M I II : 11111111 II 11 II I II I II II II 11 II II I ■ • g258 SIΝPHQFDQPLPDKEHWEQIQQTGSVRSLESIGGVLYAQGWLSAGTHΝGRDYALFFRQPI 190 200 210 220 230 240 250 260 270 280 290 300 m258.pep PKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATLLIASLLSIFLALVMALYFARRFVE M M h i M M M M M M M M M M M M I h i l l I g258 PENVAQDAVLIEKARAKYAELSYSKKGLQTFFLVTLLIASLLSIFLALVMALYFARRFVE 250 260 270 280 290 300 310 320 330 340 350 360 m258. pep PVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTKLFNHMTEQLSIAKEADERNRRREEAA h i l l I I I I I M i l l I I M M M M M M M M M M M I M M M g258 PILSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTKLFNHMTEQLSIAKEADERNRRREEAA 310 320 330 340 350 360 370 380 390 400 410 420 m258 . pep RHYLECVLEGLTTGVWFDEQGCLKTFNKAAEQILGMPLTPLWGSSRHGWHGVSAQQSLL M M g258 RHYLECVLDGLTTGVWSYPLSCCRTAVFSTCHSSPLSYFX 370 380 390 400 The following partial DNA sequence was identified in N meningitidis <SEQ ED 1027>: a258. seq 1 ATGCGCCGTT TTCTACCGAT CGCAGCCATA TGCGCCGTCG TCCTGTTGTA 51 CGGACTGACG GCGGCAACCG GCAGCACCAG TTCGCTGGCG GATTATTTCT 101 GGTGGATTGT TGCGTTCAGC GCAATGCTGC TGCTGGTGTT GTCCGCCGTT 151 TTGGCACGTT ATGTCATATT GCTGTTGAAA GACAGGCGCG ACGGCGTATT 201 CGGTTCGCAG ATTGCCAAAC GCCTTTCCGG GATGTTTACG CTGGTTGCCG 251 TACTGCCCGG CGTGTTTCTG TTCGGCGTTT CCGCACAGTT TATCAACGGC 301 ACGATTAATT CGTGGTTCGG CAACGATACC CACGAGGCGC TTGAACGCAG 351 CCTCAATTTG AGCAAGTCCG CATTGAATCT GGCGGCAGAC AACGCCCTTG 401 GCAACGCCAT CCCCGTGCAG ATAGACCTCA TCGGCGCGGC TTCCCTGCCC 451 GGGGATATGG GCAGGGTGCT GGAACATTAC GCCGGCAGCG GTTTTGCCCA 501 GCTTGCCCTG TACAATGCCG CAAGCGGCAA AATCGAAAAA AGCATCAACC 551 CGCACAAGCT CGATCAGCCG TTTCCAGGTA AGGCGCGTTG GGAAAAAATC 601 CAACAGGCGG GTTCGGTCAG GGATTTGGAA AGCATAGGCG GCGTATTGTA 651 CGCGCAGGGC TGGCTGTCGG CAGGTACGCA CAACGGGCGC GATTACGCCT 701 TGTTTTTCCG TCAGCCGGTT CCCAAAGGCG TGGCAGAGGA TGCCGTCTTA 751 ATCGAAAAGG CAAGGGCGAA ATATGCTGAG TTGAGTTACA GCAAAAAAGG 801 TTTGCAGACC TTTTTCCTGG CAACCCTGCT GATTGCCTCG CTGCTGTCGA 851 TTTTTCTTGC ACTGGTCATG GCACTGTATT TCGCCCGCCG TTTCGTCGAA 901 CCCGTCCTAT CGCTTGCCGA GGGGGCGAAG GCGGTGGCGC AAGGCGATTT 951 CAGCCAGACG CGCCCCGTGT TGCGCAACGA CGAGTTCGGA CGCTTGACCA 1001 AGTTGTTCAA CCACATGACC GAGCAGCTTT CCATCGCCAA AGAAGCAGAC 1051 GAGCGCAACC GCCGGCGCGA GGAAGCCGCC AGACATTATC TCGAATGCGT 1101 GTTGGAGGGG CTGACCACGG GCGTGGTGGT GTTTGACGAA CAAGGCTGTC 1151 TGAAAACCTT CAACAAAGCG GCGGAACAGA TTTTGGGGAT GCCGCTTACC 1201 CCCCTGTGGG GCAGCAGCCG GCACGGTTGG CACGGCGTTT CGGCGCAGCA 1251 GTCCCTGCTT GCCGAAGTGT TTGCCGCCAT CGGCGCGGCG GCAGGTACGG 1301 ACAAACCGGT CCATGTGAAA TATGCCGCGC CGGACGATGC CAAAATCCTG 1351 CTGGGCAAGG CAACCGTCCT GCCCGAAGAC AACGGCAACG GCGTGGTAAT 1401 GGTGATTGAC GACATCACCG TTTTGATACA CGCGCAAAAA GAAGCCGCGT 1451 GGGGCGAAGT GGCAAAACGG CTGGCACACG AAATCCGCAA TCCGCTCACG 1501 CCCATCCAGC TTTCTGCCGA ACGGCTGGCG TGGAAATTGG GCGGGAAGCT 1551 GGACGAGCAG GACGCGCAAA TCCTGACACG TTCGACCGAC ACCATCATCA 1601 AACAAGTGGC GGCATTAAAA GAAATGGTCG AGGCATTCCG CAATTACGCG 1651 CGTTCCCCTT CGCTCAAATT GGAAAATCAG GATTTGAACG CCTTAATCGG 1701 CGATGTGTTG GCATTGTACG AAGCTGGTCC GTGCCGGTTT GCGGCGGAAC 1751 TTGCCGGCGA ACCGCTGATG ATGGCGGCGG ATACGACCGC CATGCGGCAG 1801 GTGCTGCACA ATATTTTCAA AAATGCCGCC GAAGCGGCGG AAGAAGCCGA 1851 TGTGCCCGAA GTCAGGGTAA AATCGGAAGC GGGGCAGGAC GGACGGATTG 1901 TCCTGACAGT TTGCGACAAC GGCAAGGGGT TCGGCAGGGA AATGCTGCAC 1951 AATGCCTTCG AGCCGTATGT AACGGACAAA CCGGCTGGAA CGGGATTGGG 2001 ACTGCCCGTG GTGAAAAAAA TCATTGAAGA ACACGGCGGC CGCATCAGCC 2051 TGAGCAATCA GGATGCGGGC GGCGCGTGTG TCAGAATCAT CTTGCCAAAA 2101 ACGGTAGAAA CTTATGCGTA G This corresponds to the amino acid sequence <SEQ ID 1028; ORF 258.a>: a258.pep 1 MRRFLPIAAI CAWLLYGLT AATGSTSSLA DYFWWIVAFS AMLLLVLSAV 51 LARYVILLLK DRRDGVFGSQ IAKRLSGMFT LVAVLPGVFL FGVSAQFING 101 TINSWFGNDT HEALERSLNL SKSALNLAAD NALGNAIPVQ IDLIGAASLP 151 GDMGRVLEHY AGSGFAQLAL YNAASGKIEK SINPHKLDQP FPGKARWEKI 201 QQAGSVRDLE SIGGVLYAQG WLSAGTHNGR DYALFFRQPV PKGVAEDAVL 251 IEKARAKYAE LSYSKKGLQT FFLATLLIAS LLSIFLALVM ALYFARRFVE 301 PVLSLAEGAK AVAQGDFSQT RPVLRNDEFG RLTKLFNHMT EQLSIAKEAD 351 ERNRRREEAA RHYLECVLEG LTTGVWFDE QGCLKTFNKA AEQILGMPLT 401 PLWGSSRHGW HGVSAQQSLL AEVFAAIGAA AGTDKPVHVK YAAPDDAKIL 451 LGKATVLPED NGNGWMVID DITVLIHAQK EAAWGEVAKR LAHEIRNPLT 501 PIQLSAERLA WKLGGKLDEQ DAQILTRSTD TIIKQVAALK EMVEAFRNYA 551 RSPSLKLENQ DLNALIGDVL ALYEAGPCRF AAELAGEPLM MAADTTAMRQ 601 VLHNIFKNAA EAAEEADVPE VRVKSEAGQD GRIVLTVCDN GKGFGREMLH 651 NAFEPYVTDK PAGTGLGLPV VKKIIEEHGG RISLSNQDAG GACVRIILPK 701 TVETYA* m258/a258 99.0% identity in 584 aa overlap 10 20 30 40 50 60 m258. pep MRRFLPIAAICAWLLYGLTAATGSTSSLADYFWWIVAFSAMLLLVLSAVLARYVILLLK I II II II II I I II II I II I II II II II II I II II II I II II I II I I II I II I II I II II I a258 MRRFLPIAAICAWLLYGLTAATGSTSSLADYFWWIVAFSAMLLLVLSAVLARYVILLLK 10 20 30 40 50 60 70 80 90 100 110 120 m258. pep DRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTINSWFGNDTHEALERSLNL II II II I II I II II I II I II II II II II II I II II I II II I II II II II I II II II II II a258 DRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTINSWFGNDTHEALERSLNL 70 80 90 100 110 120 130 140 150 160 170 180 m258. pep SKSALNLAADNALGNAVPVQIDLIGAASLPGDMGRVLEHYAGSGFAQLALYNAASGKIEK I II II II I I I I II II I: II II II II II I II II II II I II II I II II II I II II II II I II a258 SKSALNLAADNALGNAIPVQIDLIGAASLPGDMGRVLEHYAGSGFAQLALYNAASGKIEK 130 140 150 160 170 180 190 200 210 220 230 240 m258. pep SINPHKLDQPFPGKARWEKIQRAGSVRDLESIGGVLYAQGWLSAGTHNGRDYALFFRQPV I II I I II I I I I II II I I II II : II I II I II II II II I II II I II II II II I II II II I II a258 SINPHKLDQPFPGKARWEKIQQAGSVRDLESIGGVLYAQGWLSAGTHNGRDYALFFRQPV 190 200 210 220 230 240 250 260 270 280 290 300 m258.pep PKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATLLIASLLSIFLALVMALYFARRFVE I I II I I II II II I II I I I I II II II I II II I I I I II II I II II I I II II I II II II I II I a258 PKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATLLIASLLSIFLALVMALYFARRFVE 250 260 270 280 290 300 310 320 330 340 350 360 m258. pep PVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTKLFNHMTEQLSIAKEADERNRRREEAA II II I I II II I II II I I I I II I II II II II II I II I II I II II II II II I II II I II II I a258 PVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTKLFNHMTEQLSIAKEADERNRRREEAA 310 320 330 340 350 360 370 380 390 400 410 420 m258. pep RHYLECVLEGLTTGVWFDEQGCLKTFNKAAEQILGMPLTPLWGSSRHGWHGVSAQQSLL I 111 I I II II I I I II II I I I II I I II II II I II II I II I I I II II II II I I I II I II II I a258 RHYLECVLEGLTTGVWFDEQGCLKTFNKAAEQILGMPLTPLWGSSRHGWHGVSAQQSLL 370 380 390 400 410 420 430 440 450 460 470 480 m258. pep AEVFAAIGAAAGTDKPVHVKYAAPDDAKILLGKATVLPEDNGNGVVMVIDDITVLIHAQK II I II II II I II II II I I II I II II II II I II I I II I II II I II II II I II II I II I I II a258 AEVFAAIGAAAGTDKPVHVKYAAPDDAKILLGKATVLPEDNGNGWMVIDDITVLIHAQK 430 440 450 460 470 480 490 500 510 520 530 540 m258. pep EAAWGEVAKRLAHEIRNPLTPIQLSAERXAXKLGGKLDEQDAQILTRSTDTIVKQVAALK II I II I II II II I II I I II II II I II I I I II I II I II I II I I II I II II I : II I I I II a258 EAAWGEVAKRLAHEIRNPLTPIQLSAERLAWKLGGKLDEQDAQILTRSTDTIIKQVAALK 490 500 510 520 530 540 550 560 570 580 589 m258. pep EMVEAFRNYARSPSLKLENQDLNALIGDVLALYEAGPCRFAADLPANRX I II II I II II II I I I II I II I I I I I II II I I II II I II I II I : I a258 EMVEAFRNYARSPSLKLENQDLNALIGDVLALYEAGPCRFAAELAGEPLMMAADTTAMRQ 550 560 570 580 590 600 a258 VLHNIFKNAAEAAEEADVPEVRVKSEAGQDGRIVLTVCDNGKGFGREMLHNAFEPYVTDK 610 620 630 640 650 660 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1029>: g259.seq 1 atgatgatgc acgcttctgt ccaaagtcgt ttcgcaccga tactttatgt 51 tttgattttc tttgccggtt ttttgaccgc gcaaatctgg ttcaatcaga 101 aagcctatac tgaagagctg cctccgcttc tgtccgcatt gtccgccgtc 151 gcgctggtgt ggctggcgtg ggcgttcgtg tcggtgcgtt caaaggctaa 201 ggcagaaaag ttctaccgcg aaaaaatgat acagaacgaa agcatacacc 251 ccgtcctgca cgcttctttg caacacttgg aacacaagcc gcaaatgctc 301 gccctgctgg tcaaaaacca cggcaaaggc atggcggaac aggtcaggtt 351 caaggcggaa gtgctgcccg acgacgaaga cgcgcgcacg attgccgccg 401 agttggcaaa aatggatatg ttcgcattgg ggacggacgc ggtcgcctcg 451 ggcgaaacct atgggcgcgt gttcgccgat attttcgagt tgtcggcggc 501 tttggaaagg cgcgcgttca aagggatact gaaactgacg gcggaatata 551 aaaaacatct tcggcgatgc ctgccgttcg gaaacggcgt tggatttggg 601 cgcgctcaat caggcgttga gggaaatctc gaaaacgccg gaaaagccta 651 a This corresponds to the amino acid sequence <SEQ ID 1030; ORF 259.ng>: g259.pep 1 MMMHASVQSR FAPILYVLIF FAGFLTAQIW FΝQKAYTEEL PPLLSALSAV 51 ALVWLAWAFV SVRSKAKAEK FYREKMIQΝE SIHPVLHASL QHLEHKPQML 101 ALLVKΝHGKG MAEQVRFKAE VLPDDEDART IAAELAKMDM FALGTDAVAS 151 GETYGRVFAD IFELSAALER RAFKGILKLT AEYKKHLRRC LPFGΝGVGFG 201 RAQSGVEGΝL EΝAGKA* The following partial DΝA sequence was identified in N meningitidis <SEQ ED 1031>: m259.seq (partial) 1 ATGATGATGC ACGCTTCTGT CCAAAGCCGT TTCGCACCGA TACTTTATGT 51 TTTGATTTTC TTTGCCGGTT TTTTGACCGC GCAAATCTGG TTCAATCAGA 101 AAGCCTATAC TGAAGAGCTG CCTCCGCTTC TGTCCGCATT GTCCGCCGTC 151 GCGCTGGTGT GGCTGGCGTG GGCGTTCGTG TCGGCGCGTT CAAAGGCCAA 201 GGCGGAAAAG TTCTACCGCG AAAAAATGAT ACAGAACGAA AGCATACACC 251 CCGTCsTGCA CGCCTCTTTG CAACACTTGG AACACAAGCC GCAAATACTC 301 GCCCTGCTGG TCAAAAACCA CGGCAAAGGG ATGGCGGAAC AGGTCAGGTT 351 CAAGGCGGAA GTGCTGCCCG ACGACGAAGA CGCGCGCACG ATTGCCGCCG 401 AGTTGGCAAA AATGGATATG TTCGCATTGG GGACkGACGC GGTCGCCTCG 451 GGCGAAACCT ATGGACGCGT GTTCGCCGAT ATTTTCGAGT TGTCGGmGGC 501 TTTGGAAGGG CGCGCGTTCA AAGGAATGTT GAAACTGACG GCGGAATATA 551 AA.AACATCT TCGGmGATGC CTGCCGTTCG GAAACGGCGT TGGAGTTGGG 601 CGCACTCAAT CAGGCGTTGC AGGAGATTTC AAAAACATCC GG.. This corresponds to the amino acid sequence <SEQ ED 1032; ORF 259>: m259.pep (partial) 1 MMMHASVQSR FAPILYVLIF FAGFLTAQIW FΝQKAYTEEL PPLLSALSAV 51 ALVWLAWAFV SARSKAKAEK FYREKMIQΝE SIHPVXHASL QHLEHKPQIL 101 ALLVKΝHGKG MAEQVRFKAE VLPDDEDART IAAELAKMDM FALGTDAVAS 151 GETYGRVFAD IFELSXALEG RAFKGMLKLT AEYKXHLRRC LPFGNGVGVG 201 RTQSGVAGDF KNIR.. Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N. gonorrhoeae ORF 259 shows 94.3% identity over a 212 aa overlap with a predicted ORF (ORF 259.ng) from N. gonorrhoeae: m259/g259 10 20 30 40 50 60 m259. pep MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLLSALSAVALVWLAWAFV MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g259 MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLLSALSAVALVWLAWAFV 10 20 30 40 50 60 70 80 90 100 110 120 m259.pep SARSKAKAEKFYREKMIQNESIHPVXHASLQHLEHKPQILALLVKNHGKGMAEQVRFKAE hMMMMMMMIMMMM M n II MM IIMMMI Mill g259 SVRSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLALLVKNHGKGMAEQVRFKAE 70 80 90 100 110 120 130 140 150 160 170 180 m259.pep VLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFADIFELSXALEGRAFKGMLKLT MMMMMMMMMMMMMMMMMMIMMMM III MllhlM! g259 VLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFADIFELSAALERRAFKGILKLT 130 140 150 160 170 180 190 200 210 m259. pep AEYKKHLRRCLPFGNGVGVGRTQSGVAGDFKNIR MMMMMMMMM Mil h-l g259 AEYKKHLRRCLPFGNGVGFGRAQSGVEGNLENAGKAX 190 200 210 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1033>: a259.sβq (partial) 1 ATGATGATGC ACGCTTCTGT CCAAAGCCGT TTCGCACCGA TACTTTATGT 51 TTTGATTTTC TTTGCCGGTT TTTTGACCGC GCAAATCTGG TTCAATCAGA 101 AAGCCTATAC TGAAGAGCTG CCTCCGCTTC TGTCCGCATT GTCCGCCGTC 151 GCGCTGGTGT GGCTGGCGTG GGCGTTCGTG TCGGCGCGTT CAAAGGCTAA 201 GGCGGAAAAG TTCTACCGCG AAAAAATGAT ACAGAACGAA AGCATACACC 251 CCGTCCTGCA CGCTTCTTTG CAACACTTGG AACACAAGCC GCAAATGCTC 301 GCCCTGCTGG TCAAAAACCA CGGCAAAGGG ATGGCGGAAC AGGTCAGGTT 351 CAAGGCGGAA GTGCTGCCCG ACGACGAAGA CGCGCGCACG ATTGCCGCCG 401 AGTTGGCAAA AATGGATATG TTTGCATTGG GGACGGACGC GGTCGCCTCG 451 GGCGAAACCT ATGGACGCGT GTTCGCCGAT ATTTTCGAGT TGTCGGCGGC 501 TTTGGAAGGG CGCGCGTTCA AAGGAATGTT GAAACTGACG GCGGAATATA 551 AAAA.CATCT TCGGCGATGC CTGCCGTTCG GAAACGGCGT TGGAGTTGGG 601 CGCGCTCAAT CAGGCGTTGC AGGAGATTTC AAAAACATCG GAAAAGTCCA 651 A This corresponds to the amino acid sequence <SEQ ID 1034; ORF 259. a>: a259.pep (partial) 1 MMMHASVQSR FAPILYVLIF FAGFLTAQIW FΝQKAYTEEL PPLLSALSAV 51 ALVWLAWAFV SARSKAKAEK FYREKMIQΝE SIHPVLHASL QHLEHKPQML 101 ALLVKΝHGKG MAEQVRFKAE VLPDDEDART IAAELAKMDM FALGTDAVAS 151 GETYGRVFAD IFELSAALEG RAFKGMLKLT AEYKXHLRRC LPFGNGVGVG 201 RAQSGVAGDF KNIGKVQ m259/a259 98.1% identity in 213 aa overlap 10 20 30 40 50 60 m259. pep MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLLSALSAVALVWLAWAFV II I I I II II I I II II I II I II II I I II I II I I II I II I II II II I II I II II I II I I I M a259 MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLLΞALSAVALVWLAWAFV 10 20 30 40 50 60 70 80 90 100 110 120 m259. pep SARSKAKAEKFYREKMIQNEΞIHPVXHASLQHLEHKPQILALLVKNHGKGMAEQVRFKAE II I II II I II II I I I I II II I I II I II I II I II I I I I : I I I II I I I I I I I I I I I II II I a259 SARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLALLVKNHGKGMAEQVRFKAE 70 80 90 100 110 120 130 140 150 160 170 180 m259. pep VLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFADIFELSXALEGRAFKGMLKLT II I I II II I II II II II II I II I II II I I I I I I I I II I I II I I I I llllllllllllll a259 VLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFADIFELSAALEGRAFKGMLKLT 130 140 150 160 170 180 190 200 210 m259.pep AEYKXHLRRCLPFGNGVGVGRTQSGVAGDFKNIR I II I I II II I I I I I I I I I I I I : I II II II II II a259 AEYKXHLRRCLPFGNGVGVGRAQSGVAGDFKNIGKVQ 190 200 210 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1035>: g259-l.seq 1 ATGATGATGC ACGCTTCTGT CCAAAGTCGT TTCGCACCGA TACTTTATGT 51 TTTGATTTTC TTTGCCGGTT TTTTGACCGC GCAAATCTGG TTCAATCAGA 101 AAGCCTATAC TGAAGAGCTG CCTCCGCTTC TGTCCGCATT GTCCGCCGTC 151 GCGCTGGTGT GGCTGGCGTG GGCGTTCGTG TCGGTGCGTT CAAAGGCTAA 201 GGCAGAAAAG TTCTACCGCG AAAAAATGAT ACAGAACGAA AGCATACACC 251 CCGTCCTGCA CGCTTCTTTG CAACACTTGG AACACAAGCC GCAAATGCTC 301 GCCCTGCTGG TCAAAAACCA CGGCAAAGGC ATGGCGGAAC AGGTCAGGTT 351 CAAGGCGGAA GTGCTGCCCG ACGACGAAGA CGCGCGCACG ATTGCCGCCG 401 AGTTGGCAAA AATGGATATG TTCGCATTGG GGACGGACGC GGTCGCCTCG 451 GGCGAAACCT ATGGGCGCGT GTTCGCCGAT ATTTTCGAGT TGTCGGCGGC 501 TTTGGAA This corresponds to the amino acid sequence <SEQ ID 1036; ORF 259-1. ng>: g259-l.pep 1 MMMHASVQSR FAPILYVLIF FAGFLTAQIW FΝQKAYTEEL PPLLSALSAV 51 ALVWLAWAFV SVRSKAKAEK FYREKMIQΝE SIHPVLHASL QHLEHKPQML 101 ALLVKΝHGKG MAEQVRFKAE VLPDDEDART IAAELAKMDM FALGTDAVAS 151 GETYGRVFAD IFELSAALE The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1037>: m259-l.seq 1 ATGATGATGC ACGCTTCTGT CCAAAGCCGT TTCGCACCGA TACTTTATGT 51 TTTGATTTTC TTTGCCGGTT TTTTGACCGC GCAAATCTGG TTCAATCAGA 101 AAGCCTATAC TGAAGAGCTG CCTCCGCTTC TGTCCGCATT GTCCGCCGTC 151 GCGCTGGTGT GGCTGGCGTG GGCGTTCGTG TCGGCGCGTT CAAAGGCCAA 201 GGCGGAAAAG TTCTACCGCG AAAAAATGAT ACAGAACGAA AGCATACACC 251 CCGTCCTGCA CGCCTCTTTG CAACACTTGG AACACAAGCC GCAAATACTC 301 GCCCTGCTGG TCAAAAACCA CGGCAAAGGG ATGGCGGAAC AGGTCAGGTT 351 CAAGGCGGAA GTGCTGCCCG ACGACGAAGA CGCGCGCACG ATTGCCGCCG 401 AGTTGGCAAA AATGGATATG TTCGCATTGG GGACGGACGC GGTCGCCTCG 451 GGCGAAACCT ATGGACGCGT GTTCGCCGAT ATTTTCGAGT TGTCGGCGGC 501 TTTGGAAGGG CGCGCGTTCA AAGGAATGTT GAAACTGACG GCGGAATATA 551 AAAACATCTT CGGCGATGCC TGCCGTTCGG AAACGGCGTT GGAGTTGGGC 601 GCACTCAATC AGGCGTTGCA GGAGATTTCA AAAACATCGG AAAAGTCCAA 651 ACGGATATTT TATTGA This corresponds to the amino acid sequence <SEQ ID 1038; ORF 259-l>: m259-l.pep 1 MMMHASVQSR FAPILYVLIF FAGFLTAQIW FΝQKAYTEEL PPLLSALSAV 51 ALVWLAWAFV SARSKAKAEK FYREKMIQΝE SIHPVLHASL QHLEHKPQIL 101 ALLVKΝHGKG MAEQVRFKAE VLPDDEDART IAAELAKMDM FALGTDAVAS 151 GETYGRVFAD IFELSAALEG RAFKGMLKLT AEYKΝIFGDA CRSETALELG 201 ALΝQALQEIS KTΞEKSKRIF Y* g259-l/ 259-l 98.8% identity in 169 aa overlap 10 20 30 40 50 60 g259-l.pep MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLLSALSAVALVWLAWAFV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I m259-l MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLLSALSAVALVWLAWAFV 10 20 30 40 50 60 70 80 90 100 110 120 g259-l.pep SVRSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLALLVKNHGKGMAEQVRFKAE I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I m259-l SARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQILALLVKNHGKGMAEQVRFKAE 70 80 90 100 110 120 130 140 150 160 169 g259-l .pep VLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFADIFELSAALE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m259-l VLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFADIFELSAALEGRAFKGMLKLT 130 140 150 160 170 180 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1039>: a259-l.seq 1 ATGATGATGC ACGCTTCTGT CCAAAGCCGT TTCGCACCGA TACTTTATGT 51 TTTGATTTTC TTTGCCGGTT TTTTGACCGC GCAAATCTGG TTCAATCAGA 101 AAGCCTATAC TGAAGAGCTG CCTCCGCTTC TGTCCGCATT GTCCGCCGTC 151 GCGCTGGTGT GGCTGGCGTG GGCGTTCGTG TCGGCGCGTT CAAAGGCTAA 201 GGCGGAAAAG TTCTACCGCG AAAAAATGAT ACAGAACGAA AGCATACACC 251 CCGTCCTGCA CGCTTCTTTG CAACACTTGG AACACAAGCC GCAAATGCTC 301 GCCCTGCTGG TCAAAAACCA CGGCAAAGGG ATGGCGGAAC AGGTCAGGTT 351 CAAGGCGGAA GTGCTGCCCG ACGACGAAGA CGCGCGCACG ATTGCCGCCG 401 AGTTGGCAAA AATGGATATG TTTGCATTGG GGACGGACGC GGTCGCCTCG 451 GGCGAAACCT ATGGACGCGT GTTCGCCGAT ATTTTCGAGT TGTCGGCGGC 501 TTTGGAAGGG CGCGCGTTCA AAGGAATGTT GAAACTGACG GCGGAATATA 551 AAAACATCTT CGGCGATGCC TGCCGTTCGG AAACGGCGTT GGAGTTGGGC 601 GCGCTCAATC AGGCGTTGCA GGAGATTTCA AAAACATCGG AAAAGTCCAA 651 ACGGATATTT TATTGA This corresponds to the amino acid sequence <SEQ ID 1040; ORF 259-l.a>: a259-l.pep 1 MMMHASVQSR FAPILYVLIF FAGFLTAQIW FΝQKAYTEEL PPLLSALSAV 51 ALVWLAWAFV SARSKAKAEK FYREKMIQΝE SIHPVLHASL QHLEHKPQML 101 ALLVKΝHGKG MAEQVRFKAE VLPDDEDART IAAELAKMDM FALGTDAVAS 151 GETYGRVFAD IFELSAALEG RAFKGMLKLT AEYKΝIFGDA CRSETALELG 201 ALΝQALQEIS KTSEKSKRIF Y* a259-l/m259-l 99.5% identity in 221 aa overlap 10 20 30 40 50 60 a259-l.pep MMMHASVQSRFAPILYVLIFFAGFLTAQIWFΝQKAYTEELPPLLSALSAVALVWLAWAFV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m259-l MMMHASVQSRFAPILYVLIFFAGFLTAQIWFΝQKAYTEELPPLLSALSAVALVWLAWAFV 10 20 30 40 50 60 70 80 90 100 110 120 a259-l.pep SARSKAKAEKFYREKMIQΝESIHPVLHASLQHLEHKPQMLALLVKΝHGKGMAEQVRFKAE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I m259-l SARSKAKAEKFYREKMIQΝESIHPVLHASLQHLEHKPQILALLVKΝHGKGMAEQVRFKAE 70 80 90 100 110 120 130 140 150 160 170 180 a259-1. pep VLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFADIFELSAALEGRAFKGMLKLT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m259-l VLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFADIFELSAALEGRAFKGMLKLT 130 140 150 160 170 180 190 200 210 220 a259-1. pep AEYKΝIFGDACRSETALELGALΝQALQEISKTSEKSKRIFYX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m259-1 AEYKΝIFGDACRΞETALELGALΝQALQEISKTSEKSKRIFYX 190 200 210 220 The following partial DΝA sequence was identified in N. gonorrhoeae <SEQ ID 1041>: g260 . seq 1 atgggtgcgg gtgtagtatt cgttgtcttt cagccgttct tcagcctgtt 51 tcgagcgttg ttcgagggcg gagtcggtat agtcgaggga gcgcacgatg 101 ccgctgaatg cgacttcttg tccgaggaat ttacccgtat ccggatcggt 151 gatgttttta ttgattcggt aggtcagata acggcccggt tctttcaggc 201 ctttggtgta aaccctggcg cctttggtgt acagcagcct gccttccggg 251 cccgagagca ggcgcggcgc ggcagcggtt tctttgcggg aaacgatttg 301 cgggtgctgc ataaagacgc ggtagaagtt gacatcgatg gcgggaatac 351 cgtatccgga cacttcctta tccggactga ttttgacgac ggggatgccg 401 tctgtctgtt ccaagccgag gcgcggttcg ccgccaacgt agcgcaacac 451 caatacctgg cccggataaa tcaggtcggg attgtggatt tgatcccggt 501 tcgcgcccca caggggggga ccattgccac gggctgtaca ggtatttgcc 551 cgaaataccc cacagggtgt cgccctgttt ga This corresponds to the amino acid sequence <SEQ ID 1042; ORF 260.ng>: g260.pep 1 MGAGWFWF QPFFSLFRAL FEGGVGIVEG AHDAAECDFL SEEFTRIRIG 51 DVFIDSVGQI TARFFQAFGV NPGAFGVQQP AFRAREQARR GSGFFAGNDL 101 RVLHKDAVEV DIDGGNTVSG HFLIRTDFDD GDAVCLFQAE ARFAANVAQH 151 QYLARINQVG IVDLIPVRAP QGGTIATGCT GICPKYPTGC RPV* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1043>: m260. seq 1 ATGGGTGCGG GTATGGTATT CGTTGTCTTT CGGCCGTTCT CCAGCCTGTT 51 TCGAGCGTTG TTCGAGGACA GAGTCGGTAT AGTCGAGGGA GCGCACGATG 101 CCGCTGAATG CGACTTCCTG CCCGAGGAAT TTACCCGTAT CCGGATCGGT 151 GATGTTTTTA TTGATTCGGT AGGTCAGGTA GCGGCCCGGC TCTTTCAGGC 201 CTTTGGTGTA AACCCTGGTG CCTTTGGTGT ACAGCAGCCT GCCTTCCGGG 251 CCCGAG rCA sGCGCGGyGC GGCAGCGGTT TCTTTGCGGG AAACGATTTG 301 CGGATGCCGC ATAAAGATGC GGTAGAAGTT GACATCGATG GCGGGAATAC 351 CGTATCCGGA CACTTCCTTA TCCGGACTCA TTTTGACGAC GGGGATGCCG 401 TCTGTCTGTT CCAAGCCGAG GCGCGGTTCG CCGTCAACGT GGCGCAACAC 451 CAATACCTGG TCCGGATAAA TCAGGTCGGG ATTGTGGATT TGATCCCGGT 501 TCGCGTyCCA CAG This corresponds to the amino acid sequence <SEQ ID 1044; ORF 260>: m260.pep 1 MGAGMVFWF RPFSSLFRAL FEDRVGIVEG AHDAAECDFL PEEFTRIRIG 51 DVFIDSVGQV AARLFQAFGV NPGAFGVQQP AFRARXXARX GSGFFAGNDL 101 RMPHKDAVEV DIDGGNTVSG HFLIRTHFDD GDAVCLFQAE ARFAVNVAQH 151 QYLVRINQVG IVDLIPVRVP Q Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 260 shows 89.5% identity over a 171 aa overlap with a predicted ORF (ORF 260.ng) from N gonorrhoeae: m260/g260 10 20 30 40 50 60 m260.pep MGAGMVFWFRPFSSLFRALFEDRVGIVEGAHDAAECDFLPEEFTRIRIGDVFIDSVGQV M M M M M M I I I I I M I I 1 1 1 1 1 I I I I I I I I 1 1 1 1 1 I I 1 1 1 I I 1 1 1 1 I I 1 1 h g260 MGAGWFWFQPFFSLFRALFEGGVGIVEGAHDAAECDFLSEEFTRIRIGDVFIDSVGQI 10 20 30 40 50 60 70 80 90 100 110 120 m260. pep AARLFQAFGVΝPGAFGVQQPAFRARXXARXGSGFFAGΝDLRMPHKDAVEVDIDGGΝTVSG M h l l l l l l l l l l l l l l l l l l l M I I 1 1 1 1 1 ! 1 1 1 1 M M M M I M M M M g260 TARFFQAFGVΝPGAFGVQQPAFRAREQARRGSGFFAGΝDLRVLHKDAVEVDIDGGΝTVSG 70 80 90 100 110 120 130 140 150 160 170 m260. pep HFLIRTHFDDGDAVCLFQAEARFAVΝVAQHQYLVRIΝQVGIVDLI VRVPQ g260 HFLIRTDFDDGDAVCLFQAEARFAAΝVAQHQYLARIΝQVGIVDLIPVRAPQGGTIATGCT 130 140 150 160 170 180 g260 GICPKYPTGCRPV 190 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1045>: a260. seq 1 ATGGGTGCGG GTATGGTATT CGTTGTCTTT CGGCCGTTCT CCAGCCTGTT 51 TCGAGCGTTG TTCGAGGACA GAGTCGGTAT AGTCGAGGGA GCGCACGATG 101 CCGCTGAATG CGACTTCCTG CCCGAGGAAT TTACCCGTAT CCGGATCGGT 151 GATGTTTTTA TTGATTCGGT AGGTCAGGTA GCGGCCCGGC TCTTTCAGGC 201 CTTTGGTGTA AACCCTGGTG CCTTTGGTGT ACAGCAGCCT GCCTTCCGGG 251 CCCGAGAGCA GGCGCGGCGC GGCAGCGGTT TCTTTGCGGG AAACGATTTG 301 CGGGTGCCGC ATAAAGATGC GGTAGAAGTT GACATCGATG GCGGGAATAC 351 CGTATCCGGA CACTTCCTTA TCCGGACTCA TTTTGACGAC GGGGATGCCG 401 TCTGTCTGTT CCAAGCCGAG GCGCGGTTCG CCGTCAACGT GGCGCAACAC 451 CAATACCTGG TCCAGATAAA TCAGGTCGGG ATTGTGGATT TGATCCCGGT 501 TCGCGTCCCA CAGGCGGCC . CCATTGCCAC GGGCTGTACA GGTATTTGCC 551 CGAAATGCCC CACAGGGTGT CGCCCTGTTT GA This corresponds to the amino acid sequence <SEQ ID 1046; ORF 260. a>: a260.pep 1 MGAGMVFVVF RPFSSLFRAL FEDRVGIVEG AHDAAECDFL PEEFTRIRIG 51 DVFIDSVGQV AARLFQAFGV NPGAFGVQQP AFRAREQARR GSGFFAGNDL 101 RVPHKDAVEV DIDGGNTVSG HFLIRTHFDD GDAVCLFQAE ARFAVNVAQH 151 QYLVQINQVG IVDLIPVRVP QAAXIATGCT GICPKCPTGC RPV* m260/a260 97.1 % identity in 171 aa overlap 10 20 30 40 50 60 m260. pep MGAGMVFVVFRPFSSLFRALFEDRVGIVEGAHDAAECDFLPEEFTRIRIGDVFIDSVGQV II I II I I II II I II I II II I II I I I I I I II I I II I I I II I I I II I II I I I I II I I I II I I a260 MGAGMVFVVFRPFSSLFRALFEDRVGIVEGAHDAAECDFLPEEFTRIRIGDVFIDSVGQV 10 20 30 40 50 60 70 80 90 100 110 120 m260. pep AARLFQAFGVNPGAFGVQQPAFRARXXARXGSGFFAGNDLRMPHKDAVEVDIDGGNTVSG I I I II I I II II I II I II II I II II I II I I II I II II I I : I I I I I I I II II II I II I I a260 AARLFQAFGVNPGAFGVQQPAFRAREQARRGSGFFAGNDLRVPHKDAVEVDIDGGNTVSG 70 80 90 100 110 120 130 140 150 160 170 m260.pep HFLIRTHFDDGDAVCLFQAEARFAVNVAQHQYLVRINQVGIVDLIPVRVPQ I II I I I II II I II I II I I I I II I II II I I I II I I : I II II II I II II I I I I a260 HFLIRTHFDDGDAVCLFQAEARFAVNVAQHQYLVQINQVGIVDLIPVRVPQAAXIATGCT 130 140 150 160 170 180 a260 GICPKCPTGCRPVX 190 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1047>: g261.seq 1 atggagcttg ggcatatcgt attccttgtg ctttgcgcgc gttcagacgg 51 cctttttact ttccagacat tccgccagcc cgcgttcgcg caagatacag 101 ctcgggcatt cgcggcagcc gccgacgata cccttgtagc aggtgtgggt 151 ctgttcgcgg atgtagtcca acacgcccat ttcgtccgcc aacgcccacg 201 tttgcgcctt ggtcaggtac atcagcggcg tgtggatttg aaaatcgtag 251 tccatcgcca gattaagggt aacgttcatg gatttgacga acacgccgcg 301 gcagtcggga tagcccgaaa aatcggtttc gcacacgccc gcgatgatgt 351 gccggatacc ctgccctttg gcaaaaatgg cggcgtaaag caggaaaagc 401 gcgttacgcc cgtccacaaa ggtattggga acgccgttgt cggcggtttc 451 gatggcggcg gtttcgatgg cggcggtttc gtccatcagg gcgttgtgcg 501 taatctgccg catcaggctc aaatcgagta cggtttgact gacacccaaa 551 tcctgcgcga tccactctgc gcgttccagc tcgacggcat ggcgttgccc 601 gtatcggaag gtgatggctt ggacgttttc gcgcccgtag gtttggattg 651 cctgaatcag gcaggtggtc gaatcctgac cgcccgagaa gatgaccaag 701 gctttttggt ttga This corresponds to the amino acid sequence <SEQ ID 1048; ORF 261.ng>: g261.pep 1 MELGHIVFLV LCARSDGLFT FQTFRQPAFA QDTARAFAAA ADDTLVAGVG 51 LFADWQHAH FVRQRPRLRL GQVHQRRVDL KIWHRQIKG NVHGFDEHAA 101 AVGIARKIGF AHARDDVPDT LPFGKNGGVK QEKRVTPVHK GIGNAWGGF 151 DGGGFDGGGF VHQGWRNLP HQAQIEYGLT DTQILRDPLC AFQLDGMALP 201 VSEGDGLDVF APVGLDCLNQ AGGRILTARE DDQGFLV* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1049>: m261. seq 1 ATGGAGCTTG GGCATATCGT ATTCCTTATG GTTTGCGCGT GTTCAGACGG 51 CCTTTTTACT TTCCAGATAT TCCGCCAGCC cGcGTTCGCG CAAGATACAG 101 CTCGGGCATT CGCGgCAGCC GCCGACGATG CCGTTATAGC AGGTGTGGGT 151 TTGCTCGCGG ATATAGTCCA GCACGCCCAT TTCGTCCGCC AACGCCCACG 201 TTTGCGCCTT GGTCAGATAC ATCAGCGGCG TGTGGATTTG AAAATCATAG 251 TCCATCGCCA AATTAAGGGT AACGTTCATC GATTTGACAA ACACGTCGCG 301 GCAGTCGGGA TAGCCGGAGA AGTCGGTTTC GCACACGCCC GCGATGATGT 351 GCCGTATCCC CTGCCCTTTG GCGTAAATCG CGGCATAGAG CAGGAAAAGC 401 gCGTTGCGGC CGTCTACAAA GGTATTCGGA ACGCCGTTTT CGGCAGTTTC 451 GATGGCGGCG GTGTCGTCCA TCAGGGCATT GTGCGTAATC TGCCGCATCA 501 GgCTcAAGTC GAGTACGGTT TGTTTGACGC CCAAATCCTG CGCAATCCAG 551 CGGGCACGTT CCAGCTCGAC GGCATGGCGT TGCCCGTATT GGAAAGTAAT 601 GGCTTGGACG TTTTCGCGCC CGTAGGTTTG GATTGCCTGA ATCAGGCAGG 651 TGGTCGAATC CTGACCGCCC GAAAAGATGA CCAAGGCTTG TTGGTTTGA This corresponds to the amino acid sequence <SEQ ID 1050; ORF 261>: m261.pep 1 MELGHIVFLM VCACSDGLFT FQIFRQPAFA QDTARAFAAA ADDAVIAGVG 51 LLADIVQHAH FVRQRPRLRL GQIHQRRVDL KIIVHRQIKG ΝVHRFDKHVA 101 AVGIAGEVGF AHARDDVPYP LPFGVΝRGIE QEKRVAAVYK GIRΝAVFGSF 151 DGGGWHQGI VRΝLPHQAQV EYGLFDAQIL RΝPAGTFQLD GMALPVLESΝ 201 GLDVFAPVGL DCLΝQAGGRI LTARKDDQGL LV* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 261 shows 79.7% identity over a 237 aa overlap with a predicted ORF (ORF 261. ng) from N gonorrhoeae: m26l/g261 10 20 30 40 50 60 m261.pep MELGHIVFLMVCACSDGLFTFQIFRQPAFAQDTARAFAAAADDAVIAGVGLLADIVQHAH IMMMIhMI I II II I II 11111 II 1111 h = M 111 h I h II 111 g261 MELGHIVFLVLCARSDGLFTFQTFRQPAFAQDTARAFAAAADDTLVAGVGLFADWQHAH 10 20 30 40 50 60 70 80 90 100 110 120 m261.pep FVRQRPRLRLGQIHQRRVDLKIIVHRQIKGΝVHRFDKHVAAVGIAGEVGFAHARDDVPYP MMMMMIhlMMMMMMIMMM g261 FVRQRPRLRLGQVHQRRVDLKIWHRQIKGΝVHGFDEHAAAVGIARKIGFAHARDDVPDT 70 80 90 100 110 120 130 140 150 160 170 m261.pep LPFGVΝRGIEQEKRVAAVYKGIRΝAVFGSFDGGGV VHQGIVRΝLPHQAQVEYGLF MM I hMlllh IMM III 1 = 1111! 111 h II I 111 II I M I 11 g261 LPFGKΝGGVKQEKRVTPVHKGIGΝAWGGFDGGGFDGGGFVHQGWRΝLPHQAQIEYGLT 130 140 150 160 170 180 180 190 200 210 220 230 m261.pep DAQILRΝPAGTFQLDGMALPVLESΝGLDVFAPVGLDCLΝQAGGRILTARKDDQGLLVX hlllhl MMIIMIM h M II II 1111111111111 II I II h II I h I II g261 DTQILRDPLCAFQLDGMALPVSEGDGLDVFAPVGLDCLΝQAGGRILTAREDDQGFLVX 190 200 210 220 230 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1051>: a261. seq 1 ATGGAGCTTG GGCATATCGT ATTCCTTATG GTTTGCGCGT GTTCAGACGG 51 CCTTTTTACT TTCCAGATAT TCCGCCAGCC CGCGTTCGCG CAAGATACAG 101 CTCGGGCATT CGCGGCAGCC GCCGACGATG CCGTTATAGC AGGTGTGGGT 151 TTGCTCGCGG ATATAGTCCA GCGCGCCCAT TTCGTCCGCC AACGCCCAAG 201 TTTGCGCCTT GGTCAGATAC ATCAGCGGCG TGTGGATTTG AAAATCATAG 251 TCCATCGCCA GATTAAGGGT AACGTTCATG GATTTGACAA ACACGTCACG 301 GCAGTCGGGA TAGCCGGAGA AGTCGGTTTC GCACACGCCC GCGATGATGT 351 GCCGTATCCC CTGCCCTTTG GCGTAAATCG CGGCATAGAG CAGGAAAAGC 401 GCGTTGCGGC CGTCTACAAA GGTATTCGGA ACGCCGTTTT CGGCAGTTTC 451 GATGGCGGCG GTGTCGTCCA TCAGGGCATT GTGCGTAATC TGCCGCATCA 501 GGCTCAAGTC GAGTACGGTT TGTTTGACGC CCAAATCCTG CGCAATCCAG 551 CGGGCACGTT CCAGCTCGAC GGCATGGCGT TGCCCGTATT GGAAAGTAAT 601 GGCTTGGACG TTTTCGCGCC CGTAGGTTTG GATTGCCTGA ATCAGGCAGG 651 TGGTCGAATC CTGACCGCCC GAAAAGATGA CCAAGGCTTT TTGGTTTGA This corresponds to the amino acid sequence <SEQ ID 1052; ORF 261. a>: a261.pep 1 MELGHIVFLM VCACSDGLFT FQIFRQPAFA QDTARAFAAA ADDAVIAGVG 51 LLADIVQRAH FVRQRPSLRL GQIHQRRVDL KIIVHRQIKG ΝVHGFDKHVT 101 AVGIAGEVGF AHARDDVPYP LPFGVΝRGIE QEKRVAAVYK GIRΝAVFGSF 151 DGGGVVHQGI VRΝLPHQAQV EYGLFDAQIL RΝPAGTFQLD GMALPVLESΝ 201 GLDVFAPVGL DCLΝQAGGRI LTARKDDQGF LV* m261/a261 97.8% identity in 232 aa overlap 10 20 30 40 50 60 m261. pep MELGHIVFLMVCACSDGLFTFQIFRQPAFAQDTARAFAAAADDAVIAGVGLLADIVQHAH I II II II I II I I I II I II I II II I II I II II I II I II II II I II I I I I I I II I I II I: I I a261 MELGHIVFLMVCACSDGLFTFQIFRQPAFAQDTARAFAAAADDAVIAGVGLLADIVQRAH 10 20 30 40 50 60 70 80 90 100 110 120 m261.pep FVRQRPRLRLGQIHQRRVDLKIIVHRQIKGΝVHRFDKHVAAVGIAGEVGFAHARDDVPYP llllll I I I II I I II I I I I I I I I I I II I I II I II I I I : II I II I I I I II I II I I I I II a261 FVRQRPSLRLGQIHQRRVDLKIIVHRQIKGΝVHGFDKHVTAVGIAGEVGFAHARDDVPYP 70 80 90 100 110 120 130 140 150 160 170 180 m261. pep LPFGVΝRGIEQEKRVAAVYKGIRΝAVFGSFDGGGWHQGIVRΝLPHQAQVEYGLFDAQIL I II II II I I I I I I II I I I II I I I I I I II I I I II I I II I I I II II I I I I II I I I I II I I I I a261 LPFGVΝRGIEQEKRVAAVYKGIRΝAVFGSFDGGGVVHQGIVRΝLPHQAQVEYGLFDAQIL 130 140 150 160 170 180 190 200 210 220 230 m261. pep RΝPAGTFQLDGMALPVLESΝGLDVFAPVGLDCLΝQAGGRILTARKDDQGLLVX II I I I I II I I II I I II I I I II II I II I II II I I II I I II II I I II I I I I : II I a261 RΝPAGTFQLDGMALPVLESΝGLDVFAPVGLDCLΝQAGGRILTARKDDQGFLVX 190 200 210 220 230 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 1053>: g263. seq 1 atggcacgtt taaccgtaca caccctcgaa accgcccccg aagccgccaa 51 accgcgcgta gaggccgtac ccaaaaacaa cggctttatc cccaacctca 101 tcggcgtatt ggcaaacgcc cccgaagctt tggcgtttta ccaagaagtc 151 ggcaagctca acgccgccaa cagcctgacc gccggcgaag tcgaagtgat 201 ccggatcatc gccgtccgca ccaaccaatg cagcttctgc gtggcagggc 251 acaccaaact cgcaaccctg aaaaaactcc tgtccgagca atccctcaat 301 gccgcccgcg ctttggcggc aggtaaatct gacgatgcca aactcggcgc 351 gcttgccgcc ttcacccaag ccgtaatggc gaaaaaaggc gcagtatccg 401 acgacgaact caacgccttc ctcgaagcgg gctacaaccg gcagcaggca 451 gtcgaagtcg taatgggcgt agccttggca actttgtgca actacgccaa 501 caacctcgcc caaaccgaaa tcaaccccaa attgcaggca tacgcctaa This corresponds to the amino acid sequence <SEQ ID 1054; ORF 263.ng>: g263.pep 1 MARLTVHTLE TAPEAAKPRV EAVPKNNGFI PNLIGVLANA PEALAFYQEV 51 GKLNAANSLT AGEVEVIRII AVRTNQCSFC VAGHTKLATL KKLLSEQSLN 101 AARALAAGKS DDAKLGALAA FTQAVMAKKG AVSDDELNAF LEAGYNRQQA 151 VEWMGVALA TLCNYANNLA QTEINPKLQA YA* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1055>: m263.seq (partial) 1 ..GCAGCAGGCG AATTTGACGA TGCCAAACTC GGCGCGCTCG CCGCCTTCAC 51 CCAAGCCGTA ATGGCGAAAA AAGGCGCGGT ATCCGACGAG GAACTCAAAG 101 CATTTTTCGA TGCGGGCTAC AACCAGCAGC AGGCAGTCGA AGTCGTGATG 151 GGCGT.AsyC TgGCAACCCT GTGCAACTAC GTCAACAACC TCGGACAAAC 201 CGAAATCAAC CCCGAATTGC AGGCTTACGC CTGA This corresponds to the amino acid sequence <SEQ ID 1056; ORF 263>: m263.pep (partial) 1 ..GCAGCAGGCG AATTTGACGA TGCCAAACTC GGCGCGCTCG CCGCCTTCAC 51 CCAAGCCGTA ATGGCGAAAA AAGGCGCGGT ATCCGACGAG GAACTCAAAG 101 CATTTTTCGA TGCGGGCTAC AACCAGCAGC AGGCAGTCGA AGTCGTGATG 151 GGCGT.AsyC TgGCAACCCT GTGCAACTAC GTCAACAACC TCGGACAAAC 201 CGAAATCAAC CCCGAATTGC AGGCTTACGC CTGA Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 263 shows 85.7% identity over a 77 aa overlap with a predicted ORF (ORF 263.ng) from N gonorrhoeae: m263/g263 10 20 30 m263. pep AAGEFDDAKLGALAAFTQAVMAKKGAVSDE l l h M M M M M M M M M M M I h g263 QCSFCVAGHTKLATLKKLLSEQSLΝAARALAAGKSDDAKLGALAAFTQAVMAKKGAVSDD 80 90 100 110 120 130 40 50 60 70 m263 . pep ELKAFFDAGYΝQQQAVEWMGVXLATLCΝYVΝΝLGQTEIΝPELQAYAX I I M h M I I I M I I I I I I I I I 1 1 1 1 1 I I M I h I I I I I h 1 1 1 I I I g263 ELΝAFLEAGYΝRQQAVEWMGVALATLCΝYAΝΝLAQTEIΝPKLQAYAX 140 150 160 170 180 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1057>: a263. seq 1 ATGGCACGTT TAACCGTACA CACCCTCGAA ACCGCCCCCG AAGCCGCCAA 51 AGCGCGCGTC GAGGCGGTAC TTCAAAACAA CGGCTTTATC CCCAACCTTA 101 TCGGCGTATT ATCAAACGCC CCCGAAGCCT TGGCGTTTTA CCAAGAAGTC 151 GGCAAGCTCA ACGCCGCCAA CAGCCTGACC GCCGGCGAAG TCGAAGTAAT 201 CCAGATTATT GCCGCCCGCA CCAACCAATG CGGCTTCTGC GTGGCAGGGC 251 ACACCAAACT CGCAACCCTG AAAAAACTCC TTTCCGAACA ATCCGTCAAA 301 GCCGCGCGCG CTTTGGCGGC AGGCGAATTT GACGATGCTA AACTCGGCGC 351 GCTCGCCGCC TTTACCCAAG CCGTAATGGC AAAAAAAGGC GCGGTATCCG 401 ACGAGGAACT CAAAGCATTT TTTGATGCGG GCTACAACCA GCAGCAGGCA 451 GTCGAAGTCG TGATGGGCGT AGCCTTGGCA ACTTTGTGCA ACTACGTCAA 501 CAACCTCGGA CAAACCGAAA TCAACCCCGA ATTGCAGGCT TACGCCTGA This corresponds to the amino acid sequence <SEQ ID 1058; ORF 263. a>: a263.pep 1 MARLTVHTLE TAPEAAKARV EAVLQΝΝGFI PΝLIGVLSΝA PEALAFYQEV 51 GKLΝAAΝSLT AGEVEVIQII AARTΝQCGFC VAGHTKLATL KKLLSEQSVK 101 AARALAAGEF DDAKLGALAA FTQAVMAKKG AVSDEELKAF FDAGYΝQQQA 151 VEWMGVALA TLCΝYVΝΝLG QTEIΝPELQA YA* m263/a263 97.4% identity in 77 aa overlap 10 20 30 m263. pep AAGEFDDAKLGALAAFTQAVMAKKGAVSDE i ii ii ii 11 ii i ii i ii i ii 111 ii 111 ii a263 QCGFCVAGHTKLATLKKLLSEQSVKAARALAAGEFDDAKLGALAAFTQAVMAKKGAVSDE 80 90 100 110 120 130 40 50 60 70 m263. pep ELKAFFDAGYNQQQAVEVVMGXXLATLCNYVNNLGQTEINPELQAYAX I II II I I II II I II II I I I I I I I I I I I I II I II I I I II I I I I I I I I a263 ELKAFFDAGYNQQQAVEVVMGVALATLCNYVNNLGQTEINPELQAYAX 140 150 160 170 180 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1059>: g264. seq 1 ttgactttaa cccgaaaaac ccttttcctc ctcaccgccg cgttcggcac 51 acactccctt cagacggcat ccgccgacgc agtggtcaag ccggaaaaac 101 tgcacgcctc cgccaaccgc agctacaaag tcgccgaatt cacgcaaacc 151 ggcaacgcct cgtggtacgg cggcaggttt cacgggcgca aaacttccgg 201 cggagaccgc tacgatatga acgcctttac cgccgcccac aaaaccctgc 251 ccatccccag ccatgtgcgc gtaaccaaca ccaaaaacgg caaaagcgtc 301 atcgtccgcg tcaacgaccg cggccccttc cacggcaacc gcatcatcga 351 cgtatccaaa gccgccgcgc aaaaattggg ctttgtcagc caagggacgg 401 cacacgtcaa aatcgaacaa atcgtcccgg gccaatccgc accggttgcc 451 gaaaacaaag acatctttat cgacttgaaa tctttcggta cggaacacga 501 agcacaagcc tatctgaacc aagccgccca aaatttcgcc gcttcgtcat 551 caagcccgaa cctctcggtt gaaaaacgcc gttacgaata cgttgtcaaa 601 atgggcccgt ttgcctcgca ggaacgcgcc gccgaagccg aagcgcaggc 651 acgcggtatg gttcgggcgg tactgacctc cggttga This corresponds to the amino acid sequence <SEQ ID 1060; ORF 264.ng>: g264.pep 1 LTLTRKTLFL LTAAFGTHSL QTASADAWK PEKLHASAΝR SYKVAEFTQT 51 GΝASWYGGRF HGRKTSGGDR YDMΝAFTAAH KTLPIPSHVR VTΝTKΝGKSV 101 IVRVΝDRGPF HGΝRIIDVSK AAAQKLGFVS QGTAHVKIEQ IVPGQSAPVA 151 EΝKDIFIDLK SFGTEHEAQA YLΝQAAQΝFA ASSSSPΝLSV EKRRYEYWK 201 MGPFASQERA AEAEAQARGM VRAVLTSG* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1061>: m264. seq 1 TTGACTTTAA CCCGAAAAAC CCTTTTCCTT CTCACCGCCG CATTCGGCAC 51 ACACTCCCTT CAGACGGCAT CCGCCGACGC AGTGGTCAAG GCAGAAAAAC 101 TGCACGCCTC CGCCAACCGC AGCTACAAAG TCGCCGGAAA ACGCTACACG 151 CCGAAAAACC AAGTCGCCGA ATTCACGCAA ACCGGCAACG CCTCGTGGTA 201 CGGCGGCAGG TTTCACGGGC GCAAAACTTC CGGCGGAGAA CGATACGATA 251 TGAACGCCTT TACCGCCGCC CACAAAACCC TGCCCATCCC CAGCTATGTG 301 CGCGTAACCA ATACCAAAAA CGGCAAAAGC GTCATCGTCC GCGTCAACGA 351 CCGCGGCCCC TTCCACGGCA ACCGCATCAT CGACGTATCC AAAGCCGCCG 401 CGCAAAAATT GGGCTTTGTC AACCAAGGGA CGGCACACGT CAAAATCGAA 451 CAAATCGTCC CGGGCCAATC CGCACCGGTT GCCGAAAACA AAGACATCTT 501 TATCGACTTG AAATCTTTCG GTACGGAACA CGAAGCACAA GCCTATCTGA 551 ACCAAGCCGC CCAAAACTTC GCCGTTTCGT CATCGGGTAC GAACCTCTCG 601 GTTGAAAAAC GCCGTTACGA ATACGTCGTC AAAATGGGAC CGTTTACCTC 651 GCAGGAACGC GCCGCCGAAG CCGAAGCTCA GGCGCGCGGT ATGGTTCGGG 701 CGGTATTGAC CGCCGGCTGA This corresponds to the amino acid sequence <SEQ ID 1062; ORF 264>: m264.pep 1 LTLTRKTLFL LTAAFGTHSL QTASADAWK AEKLHASAΝR SYKVAGKRYT 51 PKΝQVAEFTQ TGΝASWYGGR FHGRKTSGGE RYDMΝAFTAA HKTLPIPSYV 101 RVTΝTKΝGKS VIVRVΝDRGP FHGΝRIIDVS KAAAQKLGFV ΝQGTAHVKIE 151 QIVPGQSAPV AEΝKDIFIDL KSFGTEHEAQ AYLΝQAAQΝF AVSSSGTΝLS 201 VEKRRYEYW KMGPFTSQER AAEAEAQARG MVRAVLTAG* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N. gonorrhoeae ORF 264 shows 91.6% identity over a 239 aa overlap with a predicted ORF (ORF 264.ng) from N gonorrhoeae: m264/g264 10 20 30 40 50 60 m264. pep LTLTRKTLFLLTAAFGTHSLQTASADAWKAEKLHASAΝRSYKVAGKRYTPKΝQVAEFTQ MMMMMMM MM g264 LTLTRKTLFLLTAAFGTHSLQTASADAWKPEKLHASAΝRSYKVA EFTQ 10 20 30 40 70 80 90 100 110 120 m264. pep TGΝASWYGGRFHGRKTSGGERYDMΝAFTAAHKTLPIPSYVRVTΝTKΝGKSVIVRVΝDRGP g264 TGΝASWYGGRFHGRKTSGGDRYDMΝAFTAAHKTLPIPSHVRVTΝTKΝGKSVIVRVΝDRGP 50 60 70 80 90 100 130 140 150 160 170 180 m264.pep FHGΝRIIDVSKAAAQKLGFVΝQGTAHVKIEQIVPGQSAPVAEΝKDIFIDLKSFGTEHEAQ MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g264 FHGΝRIIDVSKAAAQKLGFVSQGTAHVKIEQIVPGQSAPVAEΝKDIFIDLKSFGTEHEAQ 110 120 130 140 150 160 190 200 210 220 230 240 m264 . pep AYLΝQAAQΝFAVSSSGTΝLSVEKRRYEYWKMGPFTSQERAAEAEAQARGMVRAVLTAGX 1 1 1 1 1 1 1 1 M I = 1 1 1 : M M M M M M M M I h l M M M M M M M M M I h l l g264 AYLΝQAAQΝFAASSSSPΝLSVEKRRYEYWKMGPFASQERAAEAEAQARGMVRAVLTSGX 170 180 190 200 210 220 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1063>: a264. seq 1 TTGACTTTAA CCCGAAAAAC CCTTTTCCTC CTCACCGCCG CATTCGGCAT 51 ACATTCCTTT CAGACGGCAT CCGCCGACGC AGTGGTCAGG GCAGAAAAAC 101 TGCACGCCTC CGCCAACCGC AGCTACAAAG TCGCCGGAAA ACGCTACACG 151 CCGAAAAACC AAGTCGCCGA ATTCACGCAA ACCGGCAACG CCTCGTGGTA 201 CGGCGGCAGG TTTCACGGGC GCAAAACTTC CGGCGGAGAA CGATACGATA 251 TGAACGCCTT TACCGCCGCC CACAAAACCC TGCCCATCCC CAGCTATGTG 301 CGCGTAACCA ATACCAAAAA CGGCAAAAGC GTCATCGTCC GCGTCAACGA 351 CCGCGGCCCC TTCCACGGCA ACCGCATCAT CGACGTATCC AAAGCCGCCG 401 CGCAAAAATT GGGCTTTGTC AACCAAGGGA CGGCGCACGT CAAAATCGAA 451 CAAATCGTCC CGGGCCAATC CGCACCGGTT GCCGAAAACA AAGACATCTT 501 CATCGACTTG AAATCTTTCG GTACGGAACA CGAAGCACAA GCCTATCTGA 551 ACCAAGCCGC CCAAAACCTG GCTTCATCGG CATCAAACCC GAACCTCTCG 601 GTTGAAAAAC GCCGTTACGA ATACGTCGTC AAAATGGGAC CGTTTGCCTC 651 GCAGGAACGC GCCGCCGAGG CCGAAGCTCA GGCGCGCGGT ATGGTTCGGG 701 CGGTATTAAC CGCCGGTTGA This corresponds to the amino acid sequence <SEQ ID 1064; ORF 264.a>: a264.pep 1 LTLTRKTLFL LTAAFGIHSF QTASADAVVR AEKLHASAΝR SYKVAGKRYT 51 PKΝQVAEFTQ TGΝASWYGGR FHGRKTSGGE RYDMΝAFTAA HKTLPIPSYV 101 RVTΝTKΝGKS VIVRVΝDRGP FHGΝRIIDVS KAAAQKLGFV ΝQGTAHVKIE 151 QIVPGQSAPV AEΝKDIFIDL KSFGTEHEAQ AYLΝQAAQΝL ASSASΝPΝLS 201 VEKRRYEYW KMGPFASQER AAEAEAQARG MVRAVLTAG* m264/a264 96.2% identity in 239 aa overlap 10 20 30 40 50 60 m26 . pep LTLTRKTLFLLTAAFGTHSLQTASADAWKAEKLHASAΝRSYKVAGKRYTPKΝQVAEFTQ I M I I II I I I II I I I I I I: II II II II I: I I I I I I I I I I I I I M I I I I I M M I I M I I a264 LTLTRKTLFLLTAAFGIHSFQTASADAWRAEKLHASAΝRSYKVAGKRYTPKΝQVAEFTQ 10 20 30 40 50 60 70 80 90 100 110 120 m264.pep TGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRVTNTKNGKSVIVRVNDRGP I I II I I I I I I I II I I I I I I I I II I II I I I I II I II I I II II II II II II I I II II II I I I a264 TGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRVTNTKNGKSVIVRVNDRGP 70 80 90 100 110 120 130 140 150 160 170 180 m264.pep FHGNRIIDVSKAAAQKLGFVNQGTAHVKIEQIVPGQSAPVAENKDIFIDLKSFGTEHEAQ I II I I II I II II II II I I I I I I I I I II II II I II I II II II I I I I I I II I I II II II II I a264 FHGNRIIDVSKAAAQKLGFVNQGTAHVKIEQIVPGQSAPVAENKDIFIDLKSFGTEHEAQ 130 140 150 160 170 180 190 200 210 220 230 240 m264. pep AYLNQAAQNFAVSSSGTNLSVEKRRYEYVVKMGPFTSQERAAEAEAQARGMVRAVLTAGX I II II I II I : I I : I : II I II I I II II I I II I I I : II II II II I I I I I I II II I I I I I I a264 AYLNQAAQNLASSASNPNLSVEKRRYEYWKMGPFASQERAAEAEAQARGMVRAVLTAGX 190 200 210 220 230 240 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1065>: m265. seq 1 ATGTCGGTGA TTTTGCCGCC GACACGCGCC AACGCTGCTT TTTCGGCTTG 51 GGCGCGGCTG ATGATTTTGT CTTGTTTGTT GTGTTGGTGT GCGGCGTGTC 101 CGTGGTCGTC ATCGCCGTGT CCGTCGTGGT GGGCGAGCGC GGGGGCGGAA 151 ATGCTCAGCA GTGCGGTTGC GGCGGAGGTC AAGAGAAGGT GTTTGATGTT 201 CATAT.TTTT GCCTTTGTAA ATCGTGGGTT GGAAAATGTG GATATTAATA 251 AGGTATCAAA TAACCGTCAG CCGGCGGTCA ATACCGCCCG AACCATACCG 301 CGCGCCTGAG CTTCGGCTTC GGCGGCGCGT TCCTGCGAGG TAAACGGTCC 351 CATTTTGACG ACGTATTCGT AA This corresponds to the amino acid sequence <SEQ ID 1066; ORF 265>: m265.pep 1 MSVILPPTRA ΝAAFSAWARL MILSCLLCWC AACPWSSSPC PSWWASAGAE 51 MLSSAVAAEV KRRCLMFIXF AFVΝRGLEΝV DIΝKVSΝΝRQ PAVΝTARTIP 101 RAXASASAAR SCEVΝGPILT TYS* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 265 shows 88.6% identity over a 123 aa overlap with a predicted ORF (ORF 265.ng) from N gonorrhoeae: 10 20 30 40 50 60 m265.pep MSVILPPTRAΝAAFSAWARLMILSCLLCWCAACPWSSSPCPSWWASAGAEMLSSAVAAEV M M M M M M M M M M M M M U M I g265 MSVILPPTRAQAAFSAWARLMILSCLPCWCAACPWSSSPCPSWWASAGAEMPΝSAVAAAV 10 20 30 40 50 60 70 80 90 100 110 120 m265 -pep KRRCLMFIXFAFVΝRGLEΝVDIΝKVSΝΝRQPAVΝTARTIPRAXASASAARSCEVΝGPILT I h l h l h l I M I I I I I M M M M I I I h l l M M g265 KRRCLMFI -FALVΝQGLKΝGDIΝKVSΝΝRQPEVSTARTIPRACASASAARSCEAΝGPILT 70 80 90 100 110 m265.pep TYSX MM g265 TYSX 120 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1067>: a265.seq 1 ATGTCGGTGA TTTTGCCGCC GACACGCGCC AACGCTGCTT TTTCGGCTTG 51 GGCGCGGCTG ATGATTTTGT CTTGTTTGCT GTGTTGGTGT GCGGCGTGTC 101 CGTGGTCGTC ATCGCCGTGT CCGTCGTGGT GGGCGAGTGC GGGGGCGGAA 151 ATGCCCATCA GTGCGGTTGC GGCGGCGGTC AAGAGAAGGC GTTTGAAGTT 201 CATTTTTGCT CCTGCGAAGT ATCTGGT GGTGT TTGAAGGACG 251 TAAAGGCGGG ACATCAACCG GCGGTTAATA CCGCCCGAAC CATACCGCGC 301 GCCTGAGCTT CGGCCTCGGC GGCGCGTTCC TGCGAGGCAA ACGGTCCCAT 351 TTTGACGACG TATTCGTAA This corresponds to the amino acid sequence <SEQ ID 1068; ORF 265. a>: a265.pep 1 MSVILPPTRA ΝAAFSAWARL MILSCLLCWC AACPWSSSPC PSWWASAGAE 51 MPISAVAAAV KRRRLKFIFA PAKYLX .. XC LKDVKAGHQP AVΝTARTIPR 101 A*ASASAARS CEAΝGPILTT YS* m265/a265 79.7% identity in 123 aa overlap 10 20 30 40 50 60 m265.pep MSVILPPTRAΝAAFSAWARLMILSCLLCWCAACPWSSSPCPSWWASAGAEMLSSAVAAEV I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I a265 MSVILPPTRAΝAAFSAWARLMILΞCLLCWCAACPWSSSPCPΞWWASAGAEMPISAVAAAV 10 20 30 40 50 60 70 80 90 100 110 120 m265.pep KRRCLMFIXFAFVΝRGLEΝVDIΝKVSΝΝRQPAVΝTARTIPRAXASASAARSCEVΝGPILT III I II h :: h :: I I I I I I I I I I I I I I I I II I I I I I I : I I I I I I a265 KRRRLKFI FAPAKYLXXCLKDVKAGHQPAVΝTARTIPRAXASASAARΞCEAΝGPILT 70 80 90 100 110 m265.pep TYSX a265 TYSX 120 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 1069>: g266. seq 1 agttcagacg gcatcgccgc cgacaatgcc caaacagaaa gcccatcatg 51 accgcatcca tgtacatcct tttggtcttg gcactcatct ttgccaacgc 101 ccccttcctc acgaccagac tgttcggcgt ggccgcgctc aagcgcaaac 151 atttcggaca ccacctgatc gagctggcgg caggtttcgc gctgaccgcc 201 tctcttgcct acatcctcga atcccgtgcg ggagcggtac acaatcaggg 251 ttgggagttt tacgccaccg tcgtctgcct gtacctcatt ttcgccttcc 301 cgtgtttcgt gcggcggtat ttttggcaca cgcgcaacag ggaataa This corresponds to the amino acid sequence <SEQ ID 1070; ORF 266.ng>: g266.pep 1 MQFRRHRRRQ CPΝRKPIMTA SMYILLVLAL IFAΝAPFLTT RLFGVAALKR 51 KHFGHHLIEL AAGFALTASL AYILESRAGA VHΝQGWEFYA TWCLYLIFA 101 FPCFVRRYFW HTRΝRE* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1071>: tn266. seq 1 ATGCCGTTCC GCAACGCGtT CAGACGGCAT CGCCGCCGAC AACGCCTAAA 51 CAGAAAGCCC ACCATGACCG CATCCATGTA CATCCTTTTG GTCTTGGCAC 101 TCATCTTTGC CAACGCCCCC TTCCTCACGA CCAGACTGTT CGGCGTGGCC 151 rCACTCAAGC GCAAACATTT CGGACACCAC ATGATCGAGC TGGCGGCAGG 201 TTTCGCGCTG ACCGCCGTTC TTGCCTACAT CCTsGAATCC CGTGCAGGAT 251 CGGTACACGA TCAGGGTTGG GAGTTTTATG CCACAGTCGT CTGCCTGTAC 301 CTGATTTTTG CGTTTCCATG TTTTGTGTGG CGGTATTTTT GGCACACGCG 351 CAACAGGGAA TAG This corresponds to the amino acid sequence <SEQ ID 1072; ORF 266>: m266.pep 1 MPFRNAFRRH RRRQRLNRKP TMTASMYILL VLALIFANAP FLTTRLFGVA 51 XLKRKHFGHH MIELAAGFAL TAVLAYILES RAGSVHDQGW EFYATWCLY 101 LIFAFPCFVW RYFWHTRNRE * Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 266 shows 92.1 % identity over a 114 aa overlap with a predicted ORF (ORF 266.ng) from N gonorrhoeae: m266/g266 10 20 30 40 50 60 m266. pep MPFRΝAFRRHRRRQRLΝRKPTMTASMYILLVLALIFAΝAPFLTTRLFGVAXLKRKHFGHH M M M M M M M M M M M M M I M M M g266 MQFRRHRRRQCPΝRKPIMTASMYILLVLALIFAΝAPFLTTRLFGVAALKRKHFGHH 10 20 30 40 50 70 80 90 100 110 120 m266.pep MIELAAGFALTAVLAYILESRAGSVHDQGWEFYATWCLYLIFAFPCFVWRYFWHTRΝREX M M I M M I M g266 LIELAAGFALTASLAYILESRAGAVHΝQGWEFYATWCLYLIFAFPCFVRRYFWHTRΝREX 60 70 80 90 100 110 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1073>: a266. seq 1 ATGCCGTTCC GCAATGCGTT CAGACGGCAT CGCCGCCGAC AATGCCCAAA 51 CAGAAAGCCC GCCATGACCG CATCCATGTA CATCCTTTTG CTGCTTGCCT 101 TGATTTTTGC CAACGCCCCC TTCCTCACGA CCAAGCTGTT CGGCATCGTA 151 CCGCTCAAGC GCAAACATTT CGGACACCAC CTGATCGAGC TGGCGGCAGG 201 TTTCGCGCTG ACCGCCGTTC TTGCCTACAT CCTCGAATCC CGTGCGGGAG 251 CGGTACACGA TCAGGGTTGG GAGTTTTACG CCACCGTCGT CTGCCTGTAC 301 CTGATTTTTG CGTTTCCCTG TTTCGTGTGG CGGTATTTTT GGCACACGCG 351 CAACAGGGAA TAG This corresponds to the amino acid sequence <SEQ ID 1074; ORF 266.a>: a266.pep 1 MPFRNAFRRH RRRQCPNRKP AMTASMYILL LLALIFANAP FLTTKLFGIV 51 PLKRKHFGHH LIELAAGFAL TAVLAYILES RAGAVHDQGW EFYATWCLY 101 LIFAFPCFVW RYFWHTRNRE * m266/a266 91.7% identity in 120 aa overlap 10 20 30 40 50 60 m266. pep MPFRNAFRRHRRRQRLNRKPTMTASMYILLVLALIFANAPFLTTRLFGVAXLKRKHFGHH I II II I II II I II I II I I: I II II I I II : I I I II I II I I I I I: I I I :: lllllllll a266 MPFRNAFRRHRRRQCPNRKPAMTASMYILLLLALIFANAPFLTTKLFGIVPLKRKHFGHH 10 20 30 40 50 60 70 80 90 100 110 120 m266. pep MIELAAGFALTAVLAYILESRAGSVHDQGWEFYATWCLYLIFAFPCFVWRYFWHTRNRE : I I I II II II I I M II II I I II I : I I I I II I I I I II I II II I II I I I I I I II I II II I II a266 LIELAAGFALTAVLAYILESRAGAVHDQGWEFYATWCLYLIFAFPCFVWRYFWHTRNRE 70 80 90 100 110 120 m266.pep X I a266 X The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1075>: g267. seq 1 atgcaagtcg ccttttttct cgccgtggta ttcaaaaata tgggtttcca 51 caatcgcatc ggtcgggcag gcctcttcgc agaaaccgca gaagatgcac 101 ttggtcaggt cgatgtcgta acgcttggtg cggcgggtgc cgtcttcgcg 151 ttcttccgat tcgatgttga tcgccattgc cggacacacc gcctcgcaca 201 atttacacgc gatgcagcgt tcctctccgt tcggaaaacg gcgttgcgcg 251 tgcagaccgc ggaaacgcac ggattgcggc gttttctctt cgggaaaata 301 aattgtgtct ttgcgggcaa aaaagttttt gagcgttacg cccatgcctt 351 tgaccagttc gccaagcaga aaggttttta ctaa This corresponds to the amino acid sequence <SEQ ID 1076; ORF 267.ng>: g267.pep 1 MQVAFFLAW FKNMGFHNRI GRAGLFAETA EDALGQVDW TLGAAGAVFA 51 FFRFDVDRHC RTHRLAQFTR DAAFLSVRKT ALRVQTAETH GLRRFLFGKI 101 NCVFAGKKVF ERYAHAFDQF AKQKGFY* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1077>: m267. seq 1 GTGCAAGTCG CCTTTTTTCT CGCCGTGGTA TTCAAAAATA TGGGTTTCCA 51 CAATCGCATC AGTCGGGCAT GCCTCTTCGC AGAAACCGCA GAAGATGCAC 101 TTGGTCAGGT CGATGTCGTA ACGCTTGGTG CGGCGCGTAC CGTCTTCACG 151 TTCTTCCGAT TCGATGTTAA TCGCCATTGC CGGACACACT GCCTCACACA 201 ACTTACACGC GATACACCGC TCTTCGCCGT TCGGATACCG CcGCTGCGCG 251 TGCAGACCGC GGAAACGCAC GGATTGCGGC GTTTTCTCTT CGGGGAAATA 301 AATTGTGTCT TTGCGGGCGA AAAAGTTTTT GAGCGTTACG CCCATACCTT 351 TTACCAATTC GCCAAGCAGA AAGGTTTTTA CTAA This corresponds to the amino acid sequence <SEQ ID 1078; ORF 267>: m267.pep 1 VQVAFFLAW FKΝMGFHΝRI SRACLFAETA EDALGQVDW TLGAARTVFT 51 FFRFDVΝRHC RTHCLTQLTR DTPLFAVRIP PLRVQTAETH GLRRFLFGEI 101 ΝCVFAGEKVF ERYAHTFYQF AKQKGFY* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 267 shows 82.7% identity over a 127 aa overlap with a predicted ORF (ORF 267.ng) from N gonorrhoeae: m267/g267 10 20 30 40 50 60 m267 . pep VQVAFFLAWFKΝMGFHΝRI SRACLFAETAEDALGQVDWTLGAARTVFTFFRFDVΝRHC M l l l l l l l l l l l l l l l l l h l l l l l l l l l l l l l l l l l l l l l l l M h l l l l l h l l l g267 MQVAFFLAWFKΝMGFHΝRIGRAGLFAETAEDALGQVDWTLGAAGAVFAFFRFDVDRHC 10 20 30 40 50 60 70 80 90 100 110 120 m267. pep RTHCLTQLTRDTPLFAVRIPPLRVQTAETHGLRRFLFGEIΝCVFAGEKVFERYAHTFYQF III 1 = 1 = 111= ■ II g267 RTHRLAQFTRDAAFLSVRKTALRVQTAETHGLRRFLFGKIΝCVFAGKKVFERYAHAFDQF 70 80 90 100 110 120 m267. pep AKQKGFYX l l l l l l l l g267 AKQKGFYX The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1079>: a267. seq 1 GTGCAAGTCG CCTTTTTTCT CGCCGTGGTA TTCAAAAATA TGGGTTTCCA 51 CAATCGCATC GGTCGGGCAG GCTTCTTCGC AGAAACCGCA GAAGATGCAC 101 TTGGTCAGGT CGATGTCGTA ACGCTTGGTG CGGCGCGTGC CGTCTTCGCG 151 TTCTTCCGAT TCGATGTTGA TCGCCATTGC GGGGCAAACG GCTTCACACA 201 ATTTACACGC GATGCAGCGT TCCTCGCCGT TTGGATAACG GCGTTGCGCG 251 TGCAGACCGC GGAAACGCAC GGATTGCGGC GTTTTCTCTT CGGGAAAATA 301 AATCGTGTCT TTGCGGGCAA AAAAGTTTTT GAGCGTTACG CCCATACCTT 351 TTACCAATTC GCCAAGCAGA AAGGTTTTTA CTAA This corresponds to the amino acid sequence <SEQ ID 1080; ORF 267. a>: a267.pep 1 VQVAFFLAW FKNMGFHNRI GRAGFFAETA EDALGQVDW TLGAARAVFA 51 FFRFDVDRHC GANGFTQFTR DAAFLAVWIT ALRVQTAETH GLRRFLFGKI 101 NRVFAGKKVF ERYAHTFYQF AKQKGFY* m267/a267 82.7% identity in 127 aa overlap 10 20 30 40 50 60 m267. pep VQVAFFLAWFKNMGFHNRISRACLFAETAEDALGQVDWTLGAARTVFTFFRFDVNRHC I I I I I I I I I II II II I II II : I I : I I I I I II I I I I I II I I I II I I : I I : I I I I I I : I I I a267 VQVAFFLAWFKNMGFHNRIGRAGFFAETAEDALGQVDWTLGAARAVFAFFRFDVDRHC 10 20 30 40 50 60 70 80 90 100 110 120 m267.pep RTHCLTQLTRDTPLFAVRIPPLRVQTAETHGLRRFLFGEINCVFAGEKVFERYAHTFYQF : : : I I : I II : : : I I I I I I I I II II II I I I I I I : I I I I I I : I II I I I I I I I II I a267 GANGFTQFTRDAAFLAVWITALRVQTAETHGLRRFLFGKINRVFAGKKVFERYAHTFYQF 70 80 90 100 110 120 m267.pep AKQKGFYX a267 AKQKGFYX The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 1081>: G268.seq 1 atgaaaaaaa atttacccgc actggcattg gcaagtatgc tgattttgtc 51 gggctgcgac cgtttgggaa taggcaaccc gttttccgga aaggaaattt 101 cctgcggaag cgaagagact aaagagattt tggtcaaact ggtccgcgac 151 aatgtcgaag gtgaaaccgt caaaactttt gacgacgacg cattcaaaga 201 ccaagcattt gccgatatcg gcatatcgca tatccgcaga atggtcgaac 251 gtttgggcat aaccgtcgat gaagtccgaa ctaccgagaa aaccgacacg 301 tccagcaaac tcaaatgtga agccgcgtta aaactggacg tgcccgacga 351 tgttgtcgat tatgccgtcg ccgccaacca atctataggc aacagccata 401 agaaaacgcc cgactttttt gaaccctact accgcaaaga aggcgcgtat 451 tatgtcaaaa ctatttctta cagcgtccag ccgacagacg acaaaagcaa 501 aatctttgcc gaactcagtc aggcacacga tatcatccat ccgctcagcg 551 agctggtgtc tatggcactg attaaagagc cgttggacaa agcgaaacaa 601 aggaacgaaa aacttgaagc ggcagaagcc accgcgcagg aagcgaggga 651 ggcagaagaa gcggcggcgc aggaggcatt gggtcgggag caggaagccg 701 cccgcgtatc cgaatgggaa gaacgctaca agctgtcgcg cagcgagttc 751 gagcagtttt ggaaaggatt gcctcaaact gtacagaata agctgcaagc 801 ctcgcagaaa acatggaaaa gcggtatgga caagatctgt gccaacaatg 851 cgaaagccga aggtgaaacg ccaaacggca taaaagtcag tgagttggcg 901 tgtaaaacgg cagaaaccga agcacgcttg gaagagctgc acaaccgtaa 951 aaaagccctt atcgacgaaa tggtcaggga agaggacaag aaagaactgc 1001 caaagcggct ctga This corresponds to the amino acid sequence <SEQ ID 1082; ORF 268.ng>: m268.pep 1 MKKΝLPALAL ASMLILSGCD RLGIGΝPFSG KEISCGSEET KEILVKLVRD 51 ΝVEGETVKTF DDDAFKDQAF ADIGISHIRR MVERLGITVD EVRTTEKTDT 101 SSKLKCEAAL KLDVPDDWD YAVAAΝQSIG ΝSHKKTPDFF EPYYRKEGAY 151 YVKTISYSVQ PTDDKSKIFA ELSQAHDIIH PLSELVSMAL IKEPLDKAKQ 201 RΝEKLEAAEA TAQEAREAEE AAAQEALGRE QEAARVSEWE ERYKLSRSEF 251 EQFWKGLPQT VQΝKLQASQK TWKSGMDKIC AΝΝAKAEGET PΝGIKVSELA 301 CKTAETEARL EELHΝRKKAL IDEMVREEDK KELPKRL* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1083>: m268.seq (partial) 1 ..ATGGCACTGA TTAAAGAGCC GTTGGACAAA GTGAAACAAA GGAACGAAGA 51 ACTTGAAGCG GCAGAAGAAG CGGCGGCGCA GGAGGCATTG GGTCGGGAGC 101 AGGAAGCCGC CCGCGTATCC GAATGGGAAG AACGCTACAA GCTGTCGCGC 151 AG.CAGTTCG AGCAGTTCTG GAAAGGATTG CCTCAAACCG TACAGAATAA 201 GCTGCAACCn TCACAGAAAA CATGGAAAAG CGGGATGGAT AAAATCTGTG 251 CCAACAATGC GAAAGCTGAA GGTAAAACGC CAAACGGCAT AAAATTCAGC 301 GAACTGGCAT GCAAAACGGC GAAAACCGAA GCACGCTTGG AAGAGCTGCA 351 CAACCGTAAA AAAGCCCTTA TCGACGAAAT GGyCAGGGAA GCGGACAmGA 401 AAGAACTGTC AAAGCGGCTs TGA This corresponds to the amino acid sequence <SEQ ID 1084; ORF 268>: m268.pep (partial) 1 ..MALIKEPLDK VKQRNEELEA AEEAAAQEAL GREQEAARVS EWEERYKLSR 51 XQFEQFWKGL PQTVQNKLQP SQKTWKSGMD KICANNAKAE GKTPNGIKFS 101 ELACKTAKTE ARLEELHNRK KALIDEMXRE ADXKELSKRL * Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 268 shows 86.0% identity over a 150 aa overlap with a predicted ORF (ORF 268.ng) from N gonorrhoeae: m268/g268 10 20 m268. pep MALIKEPLDKVKQRΝEELEAAE g268 SVQPTDDKSKIFAELSQAHDIIHPLSELVSMALIKEPLDKAKQRΝEKLEAAEATAQEARE 160 170 180 190 200 210 30 40 50 60 70 80 m268. pep - -EAAAQEALGREQEAARVSEWEERYKLSRSQFEQFWKGLPQTVQΝKLQPSQKTWKSGMD 111111 E 111 g268 AEEAAAQEALGREQEAARVSEWEERYKLSRSEFEQFWKGLPQTVQΝKLQASQKTWKSGMD 220 230 240 250 260 270 90 100 110 120 130 140 m268. pep KICAΝΝAKAEGKTPΝGIKFSELACKTAKTEARLEELHΝRKKALIDEMXREADXKELSKRLX II I III MM g268 KICAΝΝAKAEGETPΝGIKVSELACKTAETEARLEELHΝRKKALIDEMVREEDKKELPKRLX 280 290 300 310 320 330 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1085>: a268.seq 1 ATGGCACTGA TTAAAGAGCC GTTGGACAAA GCGAAACAAA GGAACGAAGA 51 ACTTGAAGCG GCAGAAGAAG CGGCGGCGCA GGAGGCATTG GGTCGGGAGC 101 AGGAAGTCGA CCGCGTATCC GAATGGGAAG AACGCTACAA GCTGTCGCGC 151 AGCGAGTTCG AGCAGTTCTG GAAAGGATTG CCTCAAACCG TACAGAATAA 201 GCTGCAAGCC TCACAGAAAA CATGGAAAAG CGGGATGGAT AAAATCTGTG 251 CCAACAATGC GAAAGCTGAA GGTGAAACGC CAAACGGCAT AAAATTCAGC 301 GAACTGGCAT GCAAAACGGC GGAAACCGAA GCACGCTTGG AAGAGCTGCA 351 CAACCGTAAA AAAGCCCTTC TCGACGAAAT GGCCAGGGAA GCGGACAAGA 401 AAGAACTGCC AAAGCGGCTC TGA This corresponds to the amino acid sequence <SEQ ID 1086; ORF 268. a>: a268.pep 1 MALIKEPLDK AKQRΝEELEA AEEAAAQEAL GREQEVDRVS EWEERYKLSR 51 SEFEQFWKGL PQTVQΝKLQA SQKTWKSGMD KICAΝΝAKAE GETPΝGIKFS 101 ELACKTAETE ARLEELHNRK KALLDEMARE ADKKELPKRL * m268/a268 91.4% identity in 140 aa overlap 10 20 30 40 50 60 m268.pep MALIKEPLDKVKQRNEELEAAEEAAAQEALGREQEAARVSEWEERYKLSRXQFEQFWKGL I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I h I I I I I I I I I I I I I : I I I II I I I a268 MALIKEPLDKAKQRNEELEAAEEAAAQEALGREQEVDRVSEWEERYKLSRSEFEQFWKGL 10 20 30 40 50 60 70 80 90 100 110 120 m268.pep PQTVQNKLQPSQKTWKSGMDKICANNAKAEGKTPNGIKFSELACKTAKTEARLEELHNRK l I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I : I I I I I I I I I I I I a268 PQTVQNKLQASQKTWKSGMDKICANNAKAEGETPNGIKFSELACKTAETEARLEELHNRK 70 80 90 100 110 120 130 140 m268.pep KALIDEMXREADXKELSKRLX 111 = 111 I I I I III I I I I a268 KALLDEMAREADKKELPKRLX 130 140 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 1087>: m268-l . seq 1 GTGCAATCCC GATATGATGG TTTGCATAAA TTTAAACATA TATGTTCCGC 51 AGCTATGGCA CTGATTAAAG AGCCGTTGGA CAAAGTGAAA CAAAGGAACG 101 AAGAACTTGA AGCGGCAGAA GAAGCGGCGG CGCAGGAGGC ATTGGGTCGG 151 GAGCAGGAAG CCGCCCGCGT ATCCGAATGG GAAGAACGCT ACAAGCTGTC 201 GCGCAGCGAG TTCGAGCAGT TCTGGAAAGG ATTGCCTCAA ACCGTACAGA 251 ATAAGCTGCA AGCCTCACAG AAAACATGGA AAAGCGGGAT GGATAAAATC 301 TGTGCCAACA ATGCGAAAGC TGAAGGTAAA ACGCCAAACG GCATAAAATT 351 CAGCGAACTG GCATGCAAAA CGGCGAAAAC CGAAGCACGC TTGGAAGAGC 401 TGCACAACCG TAAAAAAGCC CTTATCGACG AAATGGCCAG GGAAGCGGAC 451 AAGAAAGAAC TGTCAAAGCG GCTCTGA This corresponds to the amino acid sequence <SEQ ID 1088; ORF 268-l>: m268-l.pep 1 VQSRYDGLHK FKHICSAAMA LIKEPLDKVK QRΝEELEAAE EAAAQEALGR 51 EQEAARVSEW EERYKLSRSE FEQFWKGLPQ TVQΝKLQASQ KTWKSGMDKI 101 CAΝΝAKAEGK TPΝGIKFSEL ACKTAKTEAR LEELHΝRKKA LIDEMAREAD 151 KKELSKRL* m268-l/g268 82.3% identity in 164 aa overlap 10 20 30 m268-l.pep VQΞRYDGLHKFKHICSAAMALIKEPLDKVKQRΝE :| : I :::: I I I I I I I I I I I : I I I I I g268 KEGAYYVKTISYSVQPTDDKSKIFAELSQAHDIIHPLΞELVΞ—MALIKEPLDKAKQRΝE 150 160 170 180 190 200 40 50 60 70 80 m268-1.pep ELEAAE EAAAQEALGREQEAARVSEWEERYKLΞRSEFEQFWKGLPQTVQΝ :| I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g268 KLEAAEATAQEAREAEEAAAQEALGREQEAARVSEWEERYKLSRSEFEQFWKGLPQTVQΝ 210 220 230 240 250 260 90 100 110 120 130 140 m268-1. ep KLQASQKTWKSGMDKICAΝΝAKAEGKTPΝGIKFSELACKTAKTEARLEELHΝRKKALIDE I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I g268 KLQASQKTWKSGMDKICAΝΝAKAEGETPΝGIKVSELACKTAETEARLEELHΝRKKALIDE 270 280 290 300 310 320 150 159 m268-l.pep MAREADKKELSKRLX I : I I I I I I I I I I I g268 MVREEDKKELPKRLX 330 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1089>: a268-l.seq 1 GTGCAATCCC GATATGATGG TTTGCATAAA TTTAAACATA TATGTTCCGC 51 AGCTATGGCA CTGATTAAAG AGCCGTTGGA CAAAGCGAAA CAAAGGAACG 101 AAGAACTTGA AGCGGCAGAA GAAGCGGCGG CGCAGGAGGC ATTGGGTCGG 151 GAGCAGGAAG TCGACCGCGT ATCCGAATGG GAAGAACGCT ACAAGCTGTC 201 GCGCAGCGAG TTCGAGCAGT TCTGGAAAGG ATTGCCTCAA ACCGTACAGA 251 ATAAGCTGCA AGCCTCACAG AAAACATGGA AAAGCGGGAT GGATAAAATC 301 TGTGCCAACA ATGCGAAAGC TGAAGGTGAA ACGCCAAACG GCATAAAATT 351 CAGCGAACTG GCATGCAAAA CGGCGGAAAC CGAAGCACGC TTGGAAGAGC 401 TGCACAACCG TAAAAAAGCC CTTCTCGACG AAATGGCCAG GGAAGCGGAC 451 AAGAAAGAAC TGCCAAAGCG GCTCTGA This corresponds to the amino acid sequence <SEQ ID 1090; ORF 268-1. a>: a268-l.pep 1 VQSRYDGLHK FKHICSAAMA LIKEPLDKAK QRNEELEAAE EAAAQEALGR 51 EQEVDRVSEW EERYKLSRSE FEQFWKGLPQ TVQNKLQASQ KTWKSGMDKI 101 CANNAKAEGE TPNGIKFSEL ACKTAETEAR LEELHNRKKA LLDEMAREAD 151 KKELPKRL* a268-l/m268-l 95.6% identity in 158 aa overlap 10 20 30 40 50 60 a268-1. pep VQSRYDGLHKFKHICSAAMALIKEPLDKAKQRNEELEAAEEAAAQEALGREQEVDRVSEW I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I m268-l VQSRYDGLHKFKHICSAAMALIKEPLDKVKQRNEELEAAEEAAAQEALGREQEAARVSEW 10 20 30 40 50 60 70 80 90 100 110 120 a268-1. pep EERYKLSRSEFEQFWKGLPQTVQNKLQASQKTWKSGMDKICANNAKAEGETPNGIKFSEL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I m268-l EERYKLSRSEFEQFWKGLPQTVQNKLQASQKTWKSGMDKICANNAKAEGKTPNGIKFSEL 70 80 90 100 110 120 130 140 150 159 a268-1. pep ACKTAETEARLEELHNRKKALLDEMAREADKKELPKRLX I I I I I : I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I m268-l ACKTAKTEARLEELHNRKKALIDEMAREADKKELSKRLX 130 140 150 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1091>: g269. seq 1 atggtttggc gtgtgaattg cgcggcaacg gcggcgctga ttttttcgtc 51 cagcccttgg atttgggcgg tggtgtgggt gtggtcgcgg tcggcttttt 101 cctgcaaacc ttgcgccagc cttgacgcgt ccagtgcgcc ggcgttggcg 151 gtttcgccgt gggactttat ccggaacacg gcttcgccca aggtgtcggc 201 ggctttgatg cacagtttta aaaccagggc tttggggcgg ttttctgcgc 251 cgcccgttgc cattttgctg tccaatcgcg gggttaaaaa accgttgtcg 301 tttaagtcgc cgtccgtcca agtcgatacg agcgcgcttc tttgcctttc 351 attgcggtct tcgtaa This corresponds to the amino acid sequence <SEQ ID 1092; ORF 269.ng>: g269.pep 1 MVWRVΝCAAT AALIFSSSPW IWAWWVWSR SAFSCKPCAS LDASSAPALA 51 VSPWDFIRΝT ASPKVSAALM HSFKTRALGR FSAPPVAILL SΝRGVKKPLS 101 FKSPSVQVDT SALLCLSLRS S* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1093>: m269. seq 1 ATGGTTTGGC GTGTGAATTG CGCGGCAACG GCGGTGCTGA TTTTTTCGTC 51 CAGCCCTTGG ATTTGGGCGG CGGTGTGGGT GTGGTCTCGG TCGGCTTTGT 101 CTTGCAAACC TTGCGCCaCG TGCCCGCGTC CAGCGCCTGC GTTGATGGTT 151 TCGCCGTGGG ACTTTATCCA AAACACGGCT TCGCCCAAGG TGTCGGCGGC 201 TTTGATGCAC AGTTTTAAAA CCAGGGCTTT GGGGCGGTTT TCGTCGCCGC 251 CTGTCGCCAT TTTGCTGTCC GAGCGCGGGG TTAAAAAGCC GTTGTCGTTT 301 AAATTTTCGT CCGTCCAAGT CGATACGAGC GCGCTTCTCT GCCTTTCGTT 351 GCGGTCTTCG TAA This corresponds to the amino acid sequence <SEQ ID 1094; ORF 269>: m269.pep 1 MVWRVΝCAAT AVLIFSSSPW IWAAVWVWSR SALSCKPCAT CPRPAPALMV 51 SPWDFIQΝTA SPKVSAALMH SFKTRALGRF SSPPVAILLS ERGVKKPLSF 101 KFSSVQVDTS ALLCLSLRSS * Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 269 shows 87.6% identity over a 121 aa overlap with a predicted ORF (ORF 269.ng) from N. gonorrhoeae: m269.pep MVWRVΝCAATAVLIFSSSPWIWAAVWVWSRΞALΞCKPCATCP-RPAPALMVSPWDFIQΝT 59 llllllllllhlllllMlllhllllMlhlllllh llll llllllhll g269 MVWRVΝCAATAALIFSSSPWIWAWWVWSRSAFSCKPCASLDASSAPALAVSPWDFIRΝT 60 m269.pep ASPKVΞAALMHSFKTRALGRFSSPPVAILLΞERGVKKPLΞFKFSSVQVDTSALLCLSLRS 119 g269 ASPKVSAALMHSFKTRALGRFΞAPPVAILLSΝRGVKKPLSFKSPSVQVDTSALLCLSLRS 120 m269.pep SX 121 I I g269 SX 122 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1095>: a269.seq 1 ATGGTTTGGC GTGTGAATTG CGCGGCAACG GCGGTGCTGA TTTTTTCGTC 51 CAGCCCTTGG ATTTGGGCGG CGGTGTGGGT GTGGGCGCGG TCTGCTTTGT 101 CTTGGAGGTT TTGCGCCAGC GTGCCCGCGT CCAGCGCGCC GGCGTTGACG 151 GTTTCGCCGT GGGACTTTAT CCAGAACACG GCTTCGCCCA AGGTGTCGGC 201 GGCTTTGATG CACAGTTTTA AAACCAGGGC TTTGGGGCGG TTTTCGTCGC 251 CGCCTGTCGC CATTTTGCTG TCCGGGCGCG GGGTTAAAAA GCCGTTGTCG 301 TTTAAATTTT CGTCCGTCCA AGTCGATACG AGCGCGCTTC TCTGCCTTTC 351 GTTGTGGTCT TCGTAA This corresponds to the amino acid sequence <SEQ ID 1096; ORF 269. a>: a269.pep 1 MVWRVΝCAAT AVLIFSSSPW IWAAVWVWAR SALSWRFCAS VPASSAPALT 51 VSPWDFIQΝT ASPKVSAALM HSFKTRALGR FSSPPVAILL SGRGVKKPLS 101 FKFSSVQVDT SALLCLSLWS S* m269/a269 90.1% identity in 121 aa overlap 10 20 30 40 50 59 m269.pep MVWRVΝCAATAVLIFSΞSPWIWAAVWVWSRSALSCKPCATCP-RPAPALMVSPWDFIQΝT I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I : II: I lll l I I I I I I I I I I a269 MVWRVΝCAATAVLIFΞSSPWIWAAVWVWARΞALSWRFCAΞVPASSAPALTVSPWDFIQΝT 10 20 30 40 50 60 60 70 80 90 100 110 119 m269.pep ASPKVSAALMHSFKTRALGRFSSPPVAILLSERGVKKPLSFKFSSVQVDTSALLCLΞLRS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a269 ASPKVSAALMHSFKTRALGRFSSPPVAILLSGRGVKKPLSFKFSSVQVDTSALLCLΞLWS 70 80 90 100 110 120 120 m269.pep SX I I a269 sx The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 1097>: g270.seq 1 atgaataaaa accgcaaatt actgcttgcc gcactgctgc tgactgcctt 51 tgccgccttc aagctcgttt tgttgcaatg gtggcaggcg cagcagccgc 101 aagccgtggc ggcgcaatgc gatttgaccg agggttgcac gctgccggac 151 ggaagccgtg tccgcgccgc cgccgtttca accaaaaaac cgtttgatat 201 ttatatcgaa cacgcgcccg ccggcacgga acaggtcagc atcagcttca 251 gtatgaaaaa tatggatatg ggtttcaacc gctatatgtt cgagcggcaa 301 ccgtcgggga cttggcaggc agcacgcatc cgcctgcccg tctgtgtcga 351 aggcaggcgc gattttacgg cggacattac aatcggcagc cggacatttc 401 agacggcatt taccgccgaa taa This corresponds to the amino acid sequence <SEQ ID 1098; ORF 270.ng>: g270 . pep 1 MNKNRKLLLA ALLLTAFAAF KLVLLQWWQA QQPQAVAAQC DLTEGCTLPD 51 GSRVRAAAVS TKKPFDIYIE HAPAGTEQVS ISFSMKNMDM GFNRYMFERQ 101 PSGTWQAARI RLPVCVEGRR DFTADITIGS RTFQTAFTAE* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1099>: m270.seq 1 ATGAATAAAA ACCGTAAATT ACTGCTTGCC GCACTGCTGC TGATTGCCTT 51 TGCCGCCGTC AAGCTCGTTT TGTTGCAATG GTGGCAGGCG Ca . CAGCCGC 101 AAGCTGTGGC GGCGCAATGC GATTTGACCG AGGGTTGCAC GCTGCCGGAC 151 GGAAGCCGCG TCCGCGCCGC CGCcGTTTCA ACCAAAAAAC CGTTTGATAT 201 TTATATCGAA CACGCGCCCG CCGGCACGGA ACAGGTCAGC ATCAGCTTCA 251 GTATGAAAAA TATGGATATG GGTTTCaACC GCTATATGTT CGAGCGGCAA 301 CCGTCGGGGA CTTGGCAGGC AGTACGCATC CGCCTGCCCA TCTGTGTCGA 351 AGGCAGGCGC GATTTTACGG CGGACATTAC AATCGGCAGT CGGACATTTC 401 AGACGGCATT TACCGCCGAA TAA This corresponds to the amino acid sequence <SEQ ID 1100; ORF 270>: m270.pep 1 MΝKΝRKLLLA ALLLIAFAAV KLVLLQWWQA XQPQAVAAQC DLTEGCTLPD 51 GSRVRAAAVS TKKPFDIYIE HAPAGTEQVS ISFSMKNMDM GFNRYMFERQ 101 PSGTWQAVRI RLPICVEGRR DFTADITIGS RTFQTAFTAE * Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 270 shows 96.4% identity over a 140 aa overlap with a predicted ORF (ORF 270.ng) from N gonorrhoeae: m270/g270 10 20 30 40 50 60 m270. pep MΝKΝRKLLLAALLLIAFAAVKLVLLQWWQAXQPQAVAAQCDLTEGCTLPDGSRVRAAAVS l l l l l l l l l l l l l l l l l l l l l l l l l l l l M M M M M M M M M M M I M M M g270 MΝKΝRKLLLAALLLTAFAAFKLVLLQWWQAQQPQAVAAQCDLTEGCTLPDGSRVRAAAVS 10 20 30 40 50 60 70 80 90 100 110 120 m270.pep TKKPFDIYIEHAPAGTEQVSISFSMKΝMDMGFΝRYMFERQPSGTWQAVRIRLPICVEGRR g270 TKKPFDIYIEHAPAGTEQVSISFSMKΝMDMGFΝRYMFERQPSGTWQAARIRLPVCVEGRR 70 80 90 100 110 120 130 140 m270.pep DFTADITIGSRTFQTAFTAEX g270 DFTADITIGSRTFQTAFTAEX 130 140 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1101>: a270.seq 1 ATGAATAAAA ACCGTAAATT ACTGCTTGCC GCACTGCTGC TGATTGCCTT 51 TGCCGCCGTC AAGCTCGTTT TGTTGCAATG GTGGCAGGCG CAGCAGCCGC 101 AAGCTGTGGC GGCGCAATGC GATTTGACCG AGGGTTGCAC GCTGCCGGAC 151 GGAAGCCGCG TCCGCGCCGC CGCCGTTTCA ACCAAAAAAC CGTTTGATAT 201 TTATATCGAA CACGCGCCCG CCGGCACGGA ACAGGTCAGC ATCAGCTTCA 251 GTATGAAAAA TATGGATATG GGTTTCAACC GCTATATGTT CGAGCGGCAA 301 CCGTCGGGGA CTTGGCAGGC AGTACGCATC CGCCTGCCCA TCTGTGTCGA 351 AGGCAGGCGC GATTTTACGG CGGACATTAC AATCGGCAGC CGGACATTTC 401 AGACGGCATT TACCGCCGAA TAA This corresponds to the amino acid sequence <SEQ ID 1102; ORF 270. a>: a270.pep 1 MΝKΝRKLLLA ALLLIAFAAV KLVLLQWWQA QQPQAVAAQC DLTEGCTLPD 51 GSRVRAAAVS TKKPFDIYIE HAPAGTEQVS ISFSMKNMDM GFNRYMFERQ 101 PSGTWQAVRI RLPICVEGRR DFTADITIGS RTFQTAFTAE * m270/a270 99.3% identity in 140 aa overlap 10 20 30 40 50 60 m270. pep MNKNRKLLLAALLLIAFAAVKLVLLQWWQAXQPQAVAAQCDLTEGCTLPDGSRVRAAAVS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a270 MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTEGCTLPDGSRVRAAAVS 10 20 30 40 50 60 70 80 90 100 110 120 m270.pep TKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPSGTWQAVRIRLPICVEGRR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a270 TKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPSGTWQAVRIRLPICVEGRR 70 80 90 100 110 120 130 140 m270.pep DFTADITIGSRTFQTAFTAEX I I I I I I I I I I I I I I I I I I I I I a270 DFTADITIGSRTFQTAFTAEX 130 140 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1103>: g271.seq 1 atgttcagtt cgcggatggc gaggatttgg gcgacggggg taacgttgtg 51 tatggtcagt ccgtgtccgg cgttgacgac caagcccaaa tcgccggcga 101 aatgcgcgcc gttttggatg cgctcgaact gcctgatttg ttcggcgtgg 151 ctttgtgcgt cggcatatgc gccggtgtgc agctcgacaa cgggcgcgcc 201 gacatcacgg gcggcttgga tttgcctgtc gtcggcatcg ataaacaagg 251 acacgcgtat gcccgcgtcg gtcaggattt tggcgaattc ggcgattttt 301 tcctgttgcg ccaatacgtc caaaccgcct tcggtcgtga tttcctgccg 351 tttttcaggc acgatgcaca cgtcttccgg catcacttta agcgcgtttt 401 cgagcatttc ttccgtcaac gccatttcaa ggttcaggcg cgtgcggatg 451 gcgtttttga cggcaaatac atccgcgtct ttgatgtggc ggcggtcttc 501 gcgcaggtgc atggtaatca ggtctgcacc gtgcgtttcg gcaaccagtg 551 ccgcctccac ggggctggga taa This corresponds to the amino acid sequence <SEQ ID 1104; ORF 271.ng>: g271.pep 1 MFSSRMARIW ATGVTLCMVS PCPALTTKPK SPAKCAPFWM RSΝCLICSAW 51 LCASAYAPVC SSTTGAPTSR AAWICLSSAS IΝKDTRMPAS VRILAΝSAIF 101 SCCAΝTSKPP SWISCRFSG TMHTSSGITL SAFSSISSVΝ AISRFRRVRM 151 AFLTAΝTSAS LMWRRSSRRC MVIRSAPCVS ATSAASTGLG* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1105>: m271. seq 1 A GTTCAGTT CGCGGATGGC GAGGATTTGG GCGATGGGGG TAACGTTGTG 51 TATGGTCAGT CCGTGTCCGG CGTTGACGAC CAAGCCCAAA TCGCCGGCGA 101 AATGCGCGCC GTTTTGGATG CGCTCGAACT GCCTGATTTG TTCGGCGTGG 151 CTGCGCGCGT CGGCATACGC GCCTGTGTGC AGCTCGACAA CGGGCGCGCC 201 GACATCACGG GCGGCTTGGA TTTGCCTGTC GTCGGCATCG ATAAACAAAG 251 ACACGCGTAT GCCTGCGTCG GTCAGGATTT TGGTGAACCC GGCGATTTTT 301 TCCTGTTGCG CCAATACGTC CAAACCGCCT TCGGTCGTGA TTTCCTGACG 351 TTTTTCAGGC ACGATGCACA CGTCTTCCGG CATCACTTTC AAAGCGTTTT 401 CCAACATTTC TTCCGTCAAC GCCATTTCAA GGTTCAGGCG CGTGCGGATG 451 GCGTTTTTGA CGGCAAACAC GTCCGCGTCT TTGATGTGGC GGCGGTCTTC 501 GCGCAGGTGC ATGGTAATCA AATCCGCACC GTGCGTTTCG GCAACCAGTG 551 CCGCCTCCAC GGGGCTGGGA TAA This corresponds to the amino acid sequence <SEQ ID 1106; ORF 271>: m271.pep 1 XFSSRMARIW AMGVTLCMVS PCPALTTKPK SPAKCAPFWM RSΝCLICSAW 51 LRASAYAPVC SSTTGAPTSR AAWICLSSAS IΝKDTRMPAS VRILVΝPAIF 101 SCCAΝTSKPP SWISXRFSG TMHTSSGITF KAFSΝISSVΝ AISRFRRVRM 151 AFLTAΝTSAS LMWRRSSRRC MVIKSAPCVS ATSAASTGLG * Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 271 shows 95.2% identity over a 189 aa overlap with a predicted ORF (ORF 27 l.ng) from N gonorrhoeae: m271/g271 10 20 30 40 50 60 m271. ep XFSSRMARIWAMGVTLCMVSPCPALTTKPKSPAKCAPFWMRSΝCLICSAWLRASAYAPVC 11 II I M II I II 111111 II 11 II II II II II 11 II 111 II II 111111 g271 MFSSRMARIWATGVTLCMVSPCPALTTKPKSPAKCAPFWMRSΝCLICSAWLCASAYAPVC 10 20 30 40 50 60 70 80 90 100 110 120 m271 -pep SSTTGAPTSRAAWICLSSASIΝKDTRMPASVRILVΝPAIFSCCAΝTSKPPSWISXRFΞG llll g271 SSTTGAPTSRAAWICLSSASIΝKDTRMPASVRILAΝSAIFSCCAΝTSKPPSWISCRFSG 70 80 90 100 110 120 130 140 150 160 170 180 m271.pep TMHTSSGITFKAFSΝISSVΝAISRFRRVRMAFLTAΝTSASLMWRRSSRRCMVIKSAPCVS I I I I I I I I I :: I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I g271 TMHTSSGITLSAFSSISSVΝAISRFRRVRMAFLTAΝTSASLMWRRSSRRCMVIRSAPCVS 130 140 150 160 170 180 190 m271.pep ATSAASTGLGX I I I I I I I I I I I g271 ATSAASTGLGX 190 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1107>: a271. seq 1 ATGTTCAGTT CGCGGATGGC GAGGATTTGG GCGATGGGGG TAACGTTGTG 51 TATGGTCAGT CCGTGTCCGG CGTTGACGAC CAAGCCCAAA TCGCTGGCAA 101 AATGCGCGCC GTTTTGGATG CGCTCGAACT GCCTGATTTG TTCGGCGTGG 151 CTGCGCGCGT CGGCATACGC GCCTGTGTGC AGCTCGACAA CGGGCGCGCC 201 GACATCACGG GCGGCTTGGA TTTGCCTGTC GTCGGCATCG ATAAACAAGG 251 ACACGCGTAT GCCCGCGTCG GTCAGGATTT TGGTGAATTC GGCAATTTTG 301 TCTTGTTGCG CCAATACGTC CAAGCCGCCT TCGGTCGTGA TTTCCTGACG 351 TTTTTCCGGC ACGATGCACA CGTCTTCCGG CATCACTTTA AGCGCGTTTT 401 CGAGCATTTC TTCCGTCAAC GCCATTTCAA GGTTCAGGCG CGTGCGGATG 451 GCGTTTTTGA CAGCAAACAC GTCCGCGTCT TTGATGTGGC GGCGGTCTTC 501 GCGCAGGTGC ATGGTAATCA GGTCGGCACC GTGCGTTTCG GCAACCAGTG 551 CCGCCTCCAC GGGGCTGGGA TAA This corresponds to the amino acid sequence <SEQ ID 1108; ORF 271.a>: a271.pep 1 MFSSRMARIW AMGVTLCMVS PCPALTTKPK SLAKCAPFWM RSΝCLICSAW 51 LRASAYAPVC SSTTGAPTSR AAWICLSSAS IΝKDTRMPAS VRILVΝSAIL 101 SCCAΝTSKPP SVVIS*RFSG TMHTSSGITL SAFSSISSVΝ AISRFRRVRM 151 AFLTAΝTSAS LMWRRSSRRC MVIRSAPCVS ATSAASTGLG * m271/a271 96.3% identity in 189 aa overlap 10 20 30 40 50 60 m271.pep XFSSRMARIWAMGVTLCMVSPCPALTTKPKΞPAKCAPFWMRSΝCLICSAWLRASAYAPVC I I I I I I II I I I I I I I I I I I I I I I I I I I I II I I I II I I I I I I I I I II I I I I I I I I I I I I a271 MFSSRMARIWAMGVTLCMVSPCPALTTKPKSLAKCAPFWMRSΝCLICSAWLRASAYAPVC 10 20 30 40 50 60 70 80 90 100 110 120 m271.pep SSTTGAPTSRAAWICLSSASIΝKDTRMPASVRILVΝPAIFSCCAΝTSKPPSVVISXRFSG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I II I I I I I I I I a271 SSTTGAPTSRAAWICLΞSASIΝKDTRMPASVRILVΝSAILSCCAΝTSKPPSVVISXRFSG 70 80 90 100 110 120 130 140 150 160 170 180 m271.pep TMHTSSGITFKAFSNISSVNAISRFRRVRMAFLTANTSASLMWRRSSRRCMVIKSAPCVS I I I I I I I I I :: I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I a271 TMHTSSGITLSAFSSISSVNAISRFRRVRMAFLTANTSASLMWRRSSRRCMVIRSAPCVS 130 140 150 160 170 180 190 m271.pep ATSAASTGLGX I I I I I I I I I I I a271 ATSAASTGLGX 190 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1109>: g272.seq 1 atgactgcaa aggaagaact gttcgcatgg ctgcgccata tgaacaaaaa 51 caaaggttcc gacctgtttg tgacgaccca tttcccgccc gctatgaagc 101 tggacggcaa aatcacccgc atcacggacg aaccgctgac ggcggaaaaa 151 tgtatggaaa tcgccttttc gattatgagt gcgaagcagg cggaagaatt 201 ttcatcgacc aacgagtgca atttcgccat cagcctgccg gacaccagcc 251 gcttccgcgt caatgcgatg atacagcgcg gtgcgacggc gttggtattc 301 cgcgcgatta ccagcaagat tcccaagttt gaaagcctga acctgccgcc 351 ggccttgaag gatgttgcgc tgaaaaaacg cgggctggtt atttttgtcg 401 gcggcaccgg ctcgggcaaa tcgacttcgc tcgcctcgct tatcgactac 451 cgcaatgaaa attcgttcgg acacatcatc accatcgaag atccgatcga 501 gtttgtccac gaacacaaaa actgcatcat tacccagcgc gaggtcggcg 551 tggacacgga aaactggatg gcggcgttga aaaatacgct gcgtcaggcg 601 ccggatgtga tccttatcgg cgaaatccgc gaccgtgaaa caatggacta 651 cgccatcgcc tttgccgaaa cggggcattt gtgtatggcg acgctgcacg 701 ccaacagcac caatcaggcg ctcgaccgca tcatcaactt cttccccgag 751 gagcggcgcg aacaattgct gacggatttg tcgctcaacc ttcaggcgtt 801 tatttcgcaa cgcctcgttc cgcgagacgg cggcaagggc agggtggcgg 851 cagtcgaggt gctgctcaat tcgcccctga tttcggagtt gattcacaac 901 ggcaacatcc atgaaatcaa agaagtgatg aaaaaatcca ctaccctggg 951 tatgcagacc ttcgaccaac acctttacca attgtatgaa aaaggcgaga 1001 tttccttgca ggatgccttg aaaaatgccg attccgcaca tgatttgcgt 1051 ttggcggtac agttgcgcag ccgcagggca caaagttccg accccgattt 1101 ggaactgctc tga This corresponds to the amino acid sequence <SEQ ID 1110; ORF 272.ng>: g272.pep 1 MTAKEELFAW LRHMΝKΝKGS DLFVTTHFPP AMKLDGKITR ITDEPLTAEK 51 CMEIAFSIMS AKQAEEFSST ΝECΝFAISLP DTSRFRVΝAM IQRGATALVF 101 RAITSKIPKF ESLΝLPPALK DVALKKRGLV IFVGGTGSGK STSLASLIDY 151 RΝEΝSFGHII TIEDPIEFVH EHKΝCIITQR EVGVDTEΝWM AALKΝTLRQA 201 PDVILIGEIR DRETMDYAIA FAETGHLCMA TLHAΝSTΝQA LDRIIΝFFPE 251 ERREQLLTDL SLΝLQAFISQ RLVPRDGGKG RVAAVEVLLΝ SPLISELIHΝ 301 GΝIHEIKEVM KKSTTLGMQT FDQHLYQLYE KGEISLQDAL KΝADSAHDLR 351 LAVQLRSRRA QSSDPDLELL* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1111>: m272.seq 1 ATGACCGCAA AGGAAGAACT GTTCGCATGG CTGCGCCATA TGA CCAAAA 51 CAAAGGTTCC GACCTGTTCG TGACAACCCA TTTCCCGCCC GCAATGAAGC 101 TGGACGGCAA AATCACCCGC ATCACGGACG AACCGCTGAC GGCGGAAAAA 151 TGTATGGAAA TCGCCTTTTC GATTATGAGT GCGAAGCAGG CGGAAGAATT 201 TTCATCGACC AACGAGTGCA ACTTCGCCAT CAGCCTGCCG GACACCAGCC 251 GCTTCCGCGT CAATGCGATG ATACAGCgCG GCGCGACGGC GTTGGTATTC 301 CGTACGATTA CCAGCAAGAT TCCCAAGTTT GAAAGCCTGA ACCTGCCGCC 351 AGTCTTGAAG GATGTCGCGC TGAAAAAACG CGGGCTGGTT ATTTTTGTCG 401 GCGGCACCGG CTCGGGTAAA TCGACTTCGC TTGCCTCGCT TATCGACTAC 451 CGCAATGAAA ATTCGTTCGG ACACATCATC ACCATCGAAG ACCCGATCGA 501 GTTTGTCCAC GAACACAAAA ACTGCATCAT CACCCAGCGC GAGGTCGGCG 551 TGGATACGGA AAACTGGATG GcGGCGTTGA AAAACACGCT GCGTCAGGCG 601 CCTGATGTCA TCCTTATCGG CGAAATCCGT GACCGCGAAA CAATGGACTA 651 CGCCATTGCC TTTGCCGAAA CGGGGCATTT GTGTATGGCG ACGCTGCACG 701 CCAACAGCAC CAATCAGGCA CTCGACCGCA TCATCAACTT TTTCCCCGAG 751 GAGCGGCGCG AACAATTGCT GACGGATTTG TCGCTCAACC TTCAGGCGTT 801 TATTTCGCAA CGCCTCGTTC CGCGAGACGG CGGCAAGGGC AGGGTGGCGG 851 CAGTCGAGGT GCTGCTCAAT TCGCCCCtGA TTTCGGAGTT GATTCACAAC 901 GGCAACATCC ATGAAATCAA AGAAGTGATG AAAAAATCCA CTACCCTGGG 951 TATGCAGACC TTCGATCAAC ACCTTTACCA ATTGTATGAA AAAGGCGATA 1001 TTTCCCTGCA AGAAGCATTG AAAAATGCCG ATTCCGCACA CGATTTGCGT 1051 TTGGCGGTAC AGTTGCGCAG CCGCCGCGCG CAaAGTTyCA GCCCCGATTT 1101 GGnACTGCTC TGA This corresponds to the amino acid sequence <SEQ ID 1112; ORF 272>: m272 . pep 1 MTAKEELFAW LRHMXQNKGS DLFVTTHFPP AMKLDGKITR ITDEPLTAEK 51 CMEIAFSIMS AKQAEEFSST NECNFAISLP DTSRFRVNAM IQRGATALVF 101 RTITSKIPKF ESLNLPPVLK DVALKKRGLV IFVGGTGSGK STSLASLIDY 151 RNENSFGHII TIEDPIEFVH EHKNCIITQR EVGVDTENWM AALKNTLRQA 201 PDVILIGEIR DRETMDYAIA FAETGHLCMA TLHANSTNQA LDRIINFFPE 251 ERREQLLTDL SLNLQAFISQ RLVPRDGGKG RVAAVEVLLN SPLISELIHN 301 GNIHEIKEVM KKSTTLGMQT FDQHLYQLYE KGDISLQEAL KNADSAHDLR 351 LAVQLRSRRA QSXSPDLXLL * Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 272 shows 97.6% identity over a 370 aa overlap with a predicted ORF (ORF 272.ng) from N gonorrhoeae: m272/g272 10 20 30 40 50 60 m272. pep MTAKEELFAWLRHMXQΝKGSDLFVTTHFPPAMKLDGKITRITDEPLTAEKCMEIAFSIMS llllllllllllll : I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g272 MTAKEELFAWLRHMΝKΝKGSDLFVTTHFPPAMKLDGKITRITDEPLTAEKCMEIAFSIMS 10 20 30 40 50 60 70 80 90 100 110 120 m272. pep AKQAEEFSSTΝECΝFAISLPDTSRFRVΝAMIQRGATALVFRTITSKIPKFESLΝLPPVLK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I : I I g272 AKQAEEFSSTΝECΝFAISLPDTSRFRVΝAMIQRGATALVFRAITSKIPKFESLΝLPPALK 70 80 90 100 110 120 130 140 150 160 170 180 m272. pep DVALKKRGLVIFVGGTGSGKSTSLASLIDYRΝEΝSFGHIITIEDPIEFVHEHKΝCIITQR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I g272 DVALKKRGLVIFVGGTGSGKSTSLASLIDYRΝEΝSFGHIITIEDPIEFVHEHKΝCIITQR 130 140 150 160 170 180 190 200 210 220 230 240 m272. pep EVGVDTEΝWMAALKΝTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHAΝSTΝQA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g272 EVGVDTEΝWMAALKΝTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHAΝSTΝQA 190 200 210 220 230 240 250 260 270 280 290 300 m272.pep LDRIIΝFFPEERREQLLTDLSLΝLQAFISQRLVPRDGGKGRVAAVEVLLΝSPLISELIHΝ I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g2 2 LDRIIΝFFPEERREQLLTDLSLΝLQAFISQRLVPRDGGKGRVAAVEVLLΝSPLISELIHΝ 250 260 270 280 290 300 310 320 330 340 350 360 m272. pep GΝIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGDISLQEALKΝADSAHDLRLAVQLRSRRA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I h I I I I I I I I I I I I I I I I I I I I I I g272 GΝIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGEISLQDALKΝADSAHDLRLAVQLRSRRA 310 320 330 340 350 360 370 m272.pep QSXSPDLXLLX I I : I I I I I I g272 QSSDPDLELLX 370 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1113>: a272 . seq 1 ATGACCGCAA AGGAAGAACT GTTCGCATGG CTGCGCCATA TGAACAAAAA 51 CAAAGGTTCC GACCTGTTCG TGACGACCCA TTTCCCGCCC GCAATGAAGC 101 TGGACGGCAA AATCACCCGC ATCACGGACG AACCGCTGAC GGCGGAAAAA 151 TGTATGGAAA TCGCCTTTTC GATTATGAGT GCGAAGCAGG CGGAAGAATT 201 TTCATCGACC AACGAGTGCA ACTTCGCCAT CAGTCTGCCG GACACCAGCC 251 GCTTCCGCGT CAATGCGATG ATACAGCGCG GTGCGACGGC GTTGGTATTC 301 CGTGCGATTA CCAGCAAGAT TCCCAAGTTT GAAAGCCTGA ACCTGCCGCC 351 GGTCTTGAAG GATGTCGCGC TGAAAAAACG CGGGCTGGTT ATTTTTGTCG 401 GCGGCACCGG CTCGGGCAAA TCGACTTCGC TTGCCTCGCT TATCGACTAC 451 CGCAATGAAA ATTCGTTCGG ACACATCATC ACCATCGAAG ACCCGATCGA 501 GTTTGTCCAC GAACACAAAA ACTGCATCAT CACCCAGCGC GAGGTCGGCG 551 TGGATACGGA AAACTGGATG GCGGCGTTGA AAAACACGCT GCGTCAGGCA 601 CCGGATGTGA TTCTGATCGG CGAAATCCGC GACCGCGAAA CAATGGACTA 651 CGCCATTGCT TTTGCCGAAA CGGGGCATTT GTGTATGGCG ACGCTGCACG 701 CCAACAGCAC CAATCAGGCA CTCGACCGCA TCATCAACTT TTTCCCCGAG 751 GAGCGGCGCG AACAATTGCT GACGGATTTG TCGCTCAACC TTCAGGCATT 801 TATTTCGCAA CGCCTCGTTC CGCGAGACGG CGGCAAGGGC AGGGTGGCGG 851 CAGTCGAGGT GCTGCTCAAT TCGCCCCTGA TTTCGGAGTT GATTCACAAC 901 GGCAATATCC ATGAAATCAA AGAAGTGATG AAAAAATCCA CTACCCTGGG 951 TATGCAGACT TTCGACCAAC ACCTTTACCA ATTGTATGAA AAAGGCGAGA 1001 TTTCCTTGCA GGATGCCTTG AAAAATGCCG ATTCCGCACA CGATTTGCGT 1051 TTGGCGGTAC AGTTGCGCAG CCGCCAGGCG CAAAGTTCCG GTCCCGATTT 1101 GGAACTGCTC TGA This corresponds to the amino acid sequence <SEQID1114;ORF272.a>: a272.pep 1 MTAKEELFAW LRHMNKNKGS DLFVTTHFPP AMKLDGKITR ITDEPLTAEK 51 CMEIAFSIMS AKQAEEFSST NECNFAISLP DTSRFRVNAM IQRGATALVF 101 RAITSKIPKF ESLNLPPVLK DVALKKRGLV IFVGGTGSGK STSLASLIDY 151 RΝEΝSFGHII TIEDPIEFVH EHKNCIITQR EVGVDTENWM AALKNTLRQA 201 PDVILIGEIR DRETMDYAIA FAETGHLCMA TLHANSTNQA LDRIINFFPE 251 ERREQLLTDL SLNLQAFISQ RLVPRDGGKG RVAAVEVLLN SPLISELIHN 301 GNIHEIKEVM KKSTTLGMQT FDQHLYQLYE KGEISLQDAL KNADSAHDLR 351 LAVQLRSRQA QSSGPDLELL * m272/a272 97.6% identity in 370 aa overlap 10 20 30 40 50 60 m272 . pep MTAKEELFAWLRHMXQNKGSDLFVTTHFPPAMKLDGKITRITDEPLTAEKCMEIAFSIMS llllllllllllll : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a272 MTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKITRITDEPLTAEKCMEIAFΞIMΞ 10 20 30 40 50 60 70 80 90 100 110 120 m272.pep AKQAEEFΞΞTNECNFAISLPDTSRFRVNAMIQRGATALVFRTITSKIPKFESLNLPPVLK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I a272 AKQAEEFSΞTNECNFAISLPDTSRFRVNAMIQRGATALVFRAITSKIPKFESLNLPPVLK 70 80 90 100 110 120 130 140 150 160 170 180 m272.pep DVALKKRGLVIFVGGTGSGKSTSLASLIDYRNENSFGHIITIEDPIEFVHEHKNCIITQR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a272 DVALKKRGLVIFVGGTGSGKSTSLASLIDYRNENSFGHIITIEDPIEFVHEHKNCIITQR 130 140 150 160 170 180 190 200 210 220 230 240 m272.pep EVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHANSTNQA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a272 EVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYAIAFAETGHLCMATLHANSTNQA 190 200 210 220 230 240 250 260 . 270 280 290 300 ιrι272.pep LDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRDGGKGRVAAVEVLLNSPLISELIHN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a272 LDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRDGGKGRVAAVEVLLNSPLISELIHN 250 260 270 280 290 300 310 320 330 340 350 360 m272.pep GNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGDISLQEALKNADSAHDLRLAVQLRSRRA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I :l I I I I I I I I I I I I I I I I I I h I a272 GNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGEISLQDALKNADSAHDLRLAVQLRSRQA 310 320 330 340 350 360 370 m272.pep QSXSPDLXLLX II : I I I III a272 QSSGPDLELLX 370 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1115>: g273.seq 1 atgagtcttc aggcggtatt tatatacccc ccaagccgta ccgcacaata 51 caacgaaaat caggaaaacg gcggtaaagc tcataaacag ggacaaagcg 101 gcaaacacac cgaccgccgt caggatatag gcgtattcga ggccggaact 151 ccattcaccg ttttcctgcc gtttcttgtc gcttttgaaa taaaggatga 201 tgccggcaag cagcgcggca gccgcgcccg acattggcat tgtgttcatt 251 gttgttcctt aacggttaaa aacccgcccg gccgtgcaac cgttttaagg 301 cgggaaattg caaaatttgt ttgcgggcgc gtgccgctga aatcaaggcg 351 gtttgagaag tgtttccnac gcgcccgccc tatgtgccga aatattattt 401 gtcgctcacc tgcaaaatcg ccaagaacgc gctttgcgga atttccacgt 451 tgcccacttg tttcatacgg cgtttgcctg ctttttgttt ttcaagcagt 501 tttttcttac gcgtaa This corresponds to the amino acid sequence <SEQ ID 1116; ORF 273. ng>: g273.pep 1 MSLQAVFIYP PSRTAQYΝEΝ QEΝGGKAHKQ GQSGKHTDRR QDIGVFEAGT 51 PFTVFLPFLV AFEIKDDAGK QRGSRARHWH CVHCCSLTVK ΝPPGRATVLR 101 REIAKFVCGR VPLKSRRFEK CFXRARPMCR ΝIICRSPAKS PRTRFAEFPR 151 CPLVSYGVCL LFVFQAVFSY A* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1117>: m273.seq 1 ATGAGTCTTC AGGCGGTATT TATATACCCm CCAAGCCGTA CCGCACAATA 51 CAACGAAAAT CAGGAAAACG GCGGTAAAGC TCAyAAACAG GGACAAAGCG 101 GCAAACACGC CGACCGCTGT CAGGATATAG GCGTATTCAA GGCCGGAACT 151 CCATTCCCCG TTTTCCTGCC GCTTCTTGTC GCTTTTGAAA TAAAGGATGA 201 TGCCGGCAAG CAGCGCGGCA GCCGCGCCCG ACATTAGCAT TGTGTTCATT 251 GTTGTTCCTT AATGCTTAAA AACCCGCCTG TCCGTGCAAC CGTTTTAAGG 301 CGGCAAATTG CAAAATTTGT TTGCGGGCGC GTGCCCCTGA AATCAGGGCG 351 GTTTGAGGGG TGTTCCCGAC GCGCCGCCCT GTGTGCCGGA GTTATTTGTC 401 GCTCACCTGC AAAATCGCCA AGAACGCGCT TTGCGGAATT TCCACATTGC 451 CCACTTGTTT CATACGGCGT TTACCTGCCT TTTGTkT TC AAGCAGTTTT 501 TTCTTACGCG TAA This corresponds to the amino acid sequence <SEQ ID 1118; ORF 273>: m273.pep 1 MSLQAVFIYP PSRTAQYΝEΝ QEΝGGKAHKQ GQSGKHADRC QDIGVFKAGT 51 PFPVFLPLLV AFEIKDDAGK QRGSRARH*H CVHCCSLMLK ΝPPVRATVLR 101 RQIAKFVCGR VPLKSGRFEG CSRRAALCAG VICRSPAKSP RTRFAEFPHC 151 PLVSYGVYLP FVXQAVFSYA * Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 273 shows 86.0% identity over a 171 aa overlap with a predicted ORF (ORF 273.ng) from N gonorrhoeae: m273/g273 10 20 30 40 50 60 m273. pep MSLQAVFIYPPSRTAQYΝEΝQEΝGGKAHKQGQSGKHADRCQDIGVFKAGTPFPVFLPLLV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I: I I I I I I I I: I I I I I I I I I: I I g273 MSLQAVFIYPPSRTAQYΝEΝQEΝGGKAHKQGQSGKHTDRRQDIGVFEAGTPFTVFLPFLV 10 20 30 40 50 60 70 80 90 100 110 120 m273. pep AFEIKDDAGKQRGSRARHXHCVHCCSLMLKΝPPVRATVLRRQIAKFVCGRVPLKSGRFEG I I I I I I I I I I I I I I I I I I l l l l l l l l : I I I I I I I I I I I: I I I I I I I I g273 AFEIKDDAGKQRGSRARHWHCVHCCSLTVKΝPPGRATVLRREIAKFVCGRVPLKSRRFEK 70 80 90 100 110 120 130 140 150 160 170 m273. pep CSRRA-ALCAGVICRSPAKSPRTRFAEFPHCPLVSYGVYLPFVXQAVFSYAX I I I : I :: I I I I I I I I I I I I I I I I I : I I I I I I I I I I I l l l l l l l l g273 CFXRARPMCRΝIICRSPAKSPRTRFAEFPRCPLVSYGVCLLFVFQAVFSYAX 130 140 150 160 170 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1119>: a273. seq 1 ATGAGTCTTC AGGCGGTATT TGTATACCCC CCAAGCCGTA CCGCACAATA 51 CAACGAAAAT CAGGAAAACG GCGGTAAAGC TCATAAACAG GGACAAAGCG 101 GCAAACACGC CGACCGCCGT CAGGATATAG GCGTATTCCA GACCGGAACT 151 CCATTCACCG TTTTCCTGCC GCTTTTTGTC GCTTTTGAAA TAAAGGATGA 201 TGCCGGCAAG CAGCGCGGCA GCCGCGCCCG ACATTAGCAT AATGTTCATT 251 GTTGTTCCTT AACGGTTAAA AACCCGCCCG TCCGTGCAAC CGTTTTTAAG 301 AGGCGGTAAA TCACAAAGTT TGTTGGCGGA CGTGCTCTCT TACAATCAGG 351 GCGGTTTAAG GGGCATGATG CACTGCCCCG TGTGCCGGAT ATTATTTGTC 401 GCTCACCTGC AAAATTGCCA AGAACGCGCT TTGCGGGATT TCCACATTGC 451 CCACTTGTTT CATACGGCGT TTGCCTGCTT TTTGTTTTTC AAGCAGTTTT 501 TTCTTACGCG TAA This corresponds to the amino acid sequence <SEQ ID 1120; ORF 273. a>: a273.pep 1 MSLQAVFVYP PSRTAQYΝEΝ QEΝGGKAHKQ GQSGKHADRR QDIGVFQTGT 51 PFTVFLPLFV AFEIKDDAGK QRGSRARH*H ΝVHCCSLTVK ΝPPVRATVFK 101 RR*ITKFVGG RALLQSGRFK GHDALPRVPD IICRSPAKLP RTRFAGFPHC 151 PLVSYGVCLL FVFQAVFSYA * m273/a273 80.1 % identity in 171 aa overlap 10 20 30 40 50 60 m273.pep MSLQAVFIYPPSRTAQYΝEΝQEΝGGKAHKQGQSGKHADRCQDIGVFKAGTPFPVFLPLLV I I I I I I hi I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I :: I I I I I I I I hi a273 MSLQAVFVYPPSRTAQYΝEΝQEΝGGKAHKQGQSGKHADRRQDIGVFQTGTPFTVFLPLFV 10 20 30 40 50 60 70 80 90 100 110 119 m273.pep AFEIKDDAGKQRGSRARHXHCVHCCSLMLKΝPPVRATVL-RRQIAKFVCGRVPLKSGRFE I I I I I I II I I I I I I I I I I I I llllll :| I I I I I I I I: II hill Ih h I I I I : a273 AFEIKDDAGK Q RGSRARHXHΝVHCCSLTVKΝPPVRATVFKRRXITKFVGGRALLQSGRFK 70 80 90 100 110 120 120 130 140 150 160 170 m273.pep GCSRRAALCAGVICRSPAKSPRTRFAEFPHCPLVSYGVYLPFVXQAVFSYAX I : : : | I I I I II llllll I I I I I I I I I I I I II llllllll a273 GHDALPRV-PDIICRSPAKLPRTRFAGFPHCPLVSYGVCLLFVFQAVFSYAX 130 140 150 160 170 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1121>: g274.seq 1 ATGGCGGGGC CGATTTTTGT CGTCatCGCC AgcgTCGCTA TGTTTTTTGT 51 CGCGCAGCAG CACGCGACAG ATTTGGTTAC GGACGATTAT TATAAGGATG 101 GCAAGCATAT CGACATCCAG CTTCATCGGG ATGAAGAAGC CGTCAGACGG 151 CATATCGGGG TGCAGGTCCT CATTTCTCCC GATATGAATG CGGCAAAAGT 201 GTTTGTCGGc ggCgagtTTG ACGGCAAACA GCCTTTGAAC CTGCTGCTGA 251 TGCACCCGAC CCGCAAGGCG GACGATCAAA CCGTCGCCCT CAAGCCCGTC 301 GGCAGCGCGC AGAACGGCAG GGCGGAATAT GAGGCGGTgt tcaaAACCCT 351 TCCGCCGGCC AACCACTGGT ATGTGCGCGT GGAggacgCG GCAGGCGTGT 401 GGCGCGTCGA GAACAAATGG ATTACCAGCC AGGGCAATGC GGTCGATTTG 451 ACCCCGATGG ACAAACTTTT CAATAATGCA GGAAGCAAAT AA This corresponds to the amino acid sequence <SEQ ID 1122; ORF 274.ng>: g274.pep 1 MAGPIFWIA SVAMFFVAQQ HATDLVTDDY YKDGKHIDIQ LHRDEEAVRR 51 HIGVQVLISP DMΝAAKVFVG GEFDGKQPLΝ LLLMHPTRKA DDQTVALKPV 101 GSAQΝGRAEY EAVFKTLPPA ΝHWYVRVEDA AGVWRVEΝKW ITSQGΝAVDL 151 TPMDKLFΝΝA GSK* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1123>: m274. seq 1 ATGGCGGGGC CGATTTTTGT CGTCATCGCC AGCGTCGCTA TGTTTTTTGT 51 CGCGCAGCAG CACGCGACAG ATTTGGTTAC GGACGATTAT TATAAAGACG 101 GCAAACATAT CGACATCCAG CTTCATCGGG ATGAAGAAGC CGTCAGACGG 151 CATATCGGGG TGCAGGTTCT CATTTCCCCC GATATGAATG CGGCAAAAGT 201 GTTTGTCGGC GGCGAGTTTG ACGGCAAACA GCCTTTGAAC CTGCTGCTGA 251 TGCACCCGAC CCGCAAGGCG GACGATCAAA CCGTCGCCCT CAAGCCCGTC 301 GGCAGCGCGC AGAACGGCAG GGCGGAATAT GAGGCGGTGT TCAAAACCCT 351 TTCGCCGACC AACCACTGGT ATGTGCGCGT GGAGGACGCG GCAGGCGTGT 401 GGCGCGTCGA GAACAAATGG ATTACCAGCC AAGGCAATGC GGTCGATTTG 451 ACCCCGATGG ACAAGCTTTT CAATAATACT GAAAGCAAAT AA This corresponds to the amino acid sequence <SEQ ID 1124; ORF 274>: m274.pep 1 MAGPIFWIA SVAMFFVAQQ HATDLVTDDY YKDGKHIDIQ LHRDEEAVRR 51 HIGVQVLISP DMΝAAKVFVG GEFDGKQPLΝ LLLMHPTRKA DDQTVALKPV 101 GSAQΝGRAEY EAVFKTLSPT ΝHWYVRVEDA AGVWRVEΝKW ITSQGΝAVDL 151 TPMDKLFΝΝT ESK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 274 shows 97.5% identity over a 163 aa overlap with a predicted ORF (ORF 274.ng) from N gonorrhoeae: g274/m274 10 20 30 40 50 60 g274.pep MAGPIFWIASVAMFFVAQQHATDLVTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISP I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M m274 MAGPIFWIASVAMFFVAQQHATDLVTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISP 10 20 30 40 50 60 70 80 90 100 110 120 g274. pep DMΝAAKVFVGGEFDGKQPLΝLLLMHPTRKADDQTVALKPVGSAQΝGRAEYEAVFKTLPPA I I I I I I I I M I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : m274 DMΝAAKVFVGGEFDGK Q PLΝLLLMHPTRKADDQTVALKPVGSAQΝGRAEYEAVFKTLSPT 70 80 90 100 110 120 130 140 150 160 g274.pep ΝHWYVRVEDAAGVWRVEΝKWITSQGΝAVDLTPMDKLFΝΝAGSKX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I: I I I m274 ΝHWYVRVEDAAGVWRVEΝKWITSQGΝAVDLTPMDKLFΝΝTESKX 130 140 150 160 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1125>: a27 seq 1 ATGGCGGGGC CGATTTTTGT CGTCATCGCC AGCGTCGCTA TGTTTTTTGT 51 CGCGCAGCAG CACGCGACAG ATTTGGTTAC GGACGATTAT TATAAAGACG 101 GCAAGCATAT CGACATCCAG CTTCATCGGG ATGAAGAAGC CGTCAGACGG 151 CATATCGGGG TGCAGGTTCT CATTTCCCCC GATATGAATG CGGCAAAAGT 201 GTTTGTCGGC GGCGAGTTTG ACGGCAAACA GCCTTTGAAC CTGCTGCTGA 251 TGCACCCGAC CCGCAAGGCG GACGATCAAA CCGTCGCCCT CAAGCCCGTC 301 GGCAGCGCGC AGAACGGCAG GGCGGAATAT GAGGCGGTGT TCAAAACCCT 351 TTCGCCGACC AACCACTGGT ATGTGCGCGT GGAGGACGCG GCAGGCGTGT 401 GGCGCGTCGA GAACAAATGG ATTACCAGCC AAGGCAATGC GGTCGATTTG 451 ACCCCGATGG ACAAACTTTT CAATAATACT GAAAGCAAAT AA This corresponds to the amino acid sequence <SEQ ID 1126; ORF 274.a>: a274.pep 1 MAGPIFWIA SVAMFFVAQQ HATDLVTDDY YKDGKHIDIQ LHRDEEAVRR 51 HIGVQVLISP DMΝAAKVFVG GEFDGKQPLΝ LLLMHPTRKA DDQTVALKPV 101 GSAQΝGRAEY EAVFKTLSPT ΝHWYVRVEDA AGVWRVEΝKW ITSQGΝAVDL 151 TPMDKLFΝΝT ESK* m274/a274 100.0% identity in 163 aa overlap 10 20 30 40 50 60 m274.pep MAGPIFVVIASVAMFFVAQQHATDLVTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISP I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a274 MAGPIFVVIAΞVAMFFVAQQHATDLVTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLIΞP 10 20 30 40 50 60 70 80 90 100 110 120 m274. pep DMΝAAKVFVGGEFDGKQPLΝLLLMHPTRKADDQTVALKPVGSAQΝGRAEYEAVFKTLSPT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a274 DMΝAAKVFVGGEFDGKQPLΝLLLMHPTRKADDQTVALKPVGSAQΝGRAEYEAVFKTLΞPT 70 80 90 100 110 120 130 140 150 160 m274.pep ΝHWYVRVEDAAGVWRVEΝKWITSQGΝAVDLTPMDKLFΝΝTEΞKX I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a274 ΝHWYVRVEDAAGVWRVEΝKWITSQGΝAVDLTPMDKLFΝΝTEΞKX 130 140 150 160 The following partial DΝA sequence was identified in N.gonorrhoeae <SEQ ID 1127>: g276.seq 1 atgattttgc cgccatccat gacgatgatg cggtcggcgg attcgacggt 51 ggtcaggcgg tgggcgacga tgatgccggt gcggttttcc atcaggcgtt 101 cgagcgcttg ttggacgagg cgttcggatt cgttgtccaa tgcgctggtg 151 gcttcgtcca ataataatat cggcgcgtct ttcaaaatgg cgcgggcgat 201 ggcgacgcgt tgccgctgtc cgccggataa gttgctgccg ttcgatccga 251 tgggctggtg cagtccgagc ggggatgcgt cgatcaggct ttgcaggttg 301 gcggcttgga gggcggacag gacttcggct tcgcccgcgt cgggacggct 351 gtatcggacg ttttcaaaca gggtgtcgtc aaacaggaat acgtcttggg 401 agacgagggc gaattgggcg cgcaggcagt cgagtttgat gtcggcgatg 451 tcgataccgt ctatgcagat gttgccggca gacggttcga caaagcgggg 501 cagaaggttg acgacggtgg atttgccgct gccggaacgt ccgaccaggg 551 cgacgcgttc gccttgtctg atgtcgaggt tgaagttgtc gagggctttg 601 atgccgtctg aacggtattc gacatcgacg ttgcggaagc tgatgcgccc 651 ttcgacacgc tgcggcgcga gcgtgccttt gtcctgttcg ggcggggtgt 701 cgagaaatgc acatacgccg tcggcggcga ggaacatcgt ctgcataggg 751 atgctgatgt tggcaaggct tttgatgggg gcgtacattt gcagcatcgc 801 gacgatgaat gccataaatt cgccgatggt ggtgtag This corresponds to the amino acid sequence <SEQ ID 1128; ORF 276.ng>: g276.pep 1 MILPPSMTMM RSADSTWRR WATMMPVRFS IRRSSACWTR RSDSLSNALV 51 ASSNNNIGAS FKMARAMATR CRCPPDKLLP FDPMGWCSPS GDASIRLCRL 101 AAWRADRTSA SPASGRLYRT FSNRVSSNRN TSWETRANWA RRQSSLMSAM 151 SIPSMQMLPA DGSTKRGRRL TTVDLPLPER PTRATRSPCL MSRLKLSRAL 201 MPSERYSTST LRKLMRPSTR CGASVPLSCS GGVSRNAHTP SAARNIVCIG 251 MLMLARLLMG AYICSIATMN AINSPMW* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 1129>: m276. seq 1 ATGATTTTGC CGTCGTCCAT CACGATGATG CGGTCGGCCC CTTCGATGGT 51 GGTCAGGCGG TGGGCGACGA TGATGCCGGT GCGGTTTTCC ATCAGGCGTT 101 CGAGCGCCTG TTGGACGAGG CGTTCGGATT CGTTGTCTAA TGCGCTGGTG 151 GCTTCGTCCA ATAATAATAT CGGCGCGTCT TTCAAAATGG CGCGGGCAAT 201 GGCGACGCGT TGCCGCTGTC CGCCGGATAA GTTGCTGCCG TTCGATCCGA 251 TGGGCTGGTG CAGTCCGAGC GGGGAGCTGT CAATCAGGCT TTGCAGGTTG 301 GCGGTTTGGA GGGCGAACAG GACTTCGGCT TCGCCCGCGT CGGGACGGCT 351 GTATCGGACG TTTTCAAACA GGGTGTCGTC AAACAGGAAT ACGTCTTGGG 401 AGACGAGGGC GAATTGGGCG CGCAGGCAGT CGAGTTTGAT GTCGGCGATG 451 TCGATACCGT CTATGCAGAT GTTGCCGGCA GACGGTTCGA CAAAGCGGGG 501 CAGCAGGTTG ACGACGGTGG ATTTGCCGCT GCCGGAACGT CCGACCAGGG 551 CGACGCGTTC GCCTTGTCTG ATGTCGAGGT TGAAGTTGTC GAGGGCTTTG 601 ATGCCGTCTG AACGGTATTC GACATCGACG TTGCGGAAGC TGATGCGCCC 651 TTCGACACGC TGCGGTGCGA GCGTGCCCTT GTCCTGTTCG GGCGGGGTGT 701 CGAGAAATGC ACATACACCG TCGGCGGCGA GGAACATCGT CTGCATAGGG 751 ATGCTGATGT TGGCAAGGCT TTTGATGGGG GCGTACATTT GCAGCATCGC 801 GACGATGAAT GCCATAAATT CGCCGATGGT GGTGTAG This corresponds to the amino acid sequence <SEQ ID 1130; ORF 276>: m276.pep 1 MILPSSITMM RSAPSMWRR WATMMPVRFS IRRSSACWTR RSDSLSNALV 51 ASSNNNIGAS FKMARAMATR CRCPPDKLLP FDPMGWCSPS GELSIRLCRL 101 AVWRANRTSA SPASGRLYRT FSNRVSSNRN TSWETRANWA RRQSSLMSAM 151 SIPSMQMLPA DGSTKRGSRL TTVDLPLPER PTRATRSPCL MSRLKLSRAL 201 MPSERYSTST LRKLMRPSTR CGASVPLSCS GGVSRNAHTP SAARNIVCIG 251 MLMLARLLMG AYICSIATMN AINSPMW* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 276 shows 96.8% identity over a 278 aa overlap with a predicted ORF (ORF 276.ng) from N gonorrhoeae: m276/g276 10 20 30 40 50 60 m276.pep MILPSSITMMRSAPSMWRRWATMMPVRFSIRRSSACWTRRSDSLSΝALVASSΝΝΝIGAS llll I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g276 MILPPSMTMMRSADSTWRRWATMMPVRFSIRRSSACWTRRSDSLSΝALVASSΝΝΝIGAS 10 20 30 40 50 60 70 80 90 100 110 120 m276. pep FKMARAMATRCRCPPDKLLPFDPMGWCΞPSGELSIRLCRLAVWRAΝRTSASPASGRLYRT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I : I I I : I I I I I I I I I I I I I I g276 FKMARAMATRCRCPPDKLLPFDPMGWCSPSGDASIRLCRLAAWRADRTΞASPASGRLYRT 70 80 90 100 110 120 130 140 150 160 170 180 m276.pep FSΝRVSSΝRΝTSWETRAΝWARRQSSLMSAMSIPSMQMLPADGSTKRGSRLTTVDLPLPER I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g276 FSΝRVSSΝRΝTSWETRAΝWARRQSSLMSAMSIPSMQMLPADGSTKRGRRLTTVDLPLPER 130 140 150 160 170 180 190 200 210 220 230 240 m276.pep PTRATRSPCLMSRLKLSRALMPSERYSTSTLRKLMRPSTRCGASVPLSCSGGVSRΝAHTP M I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g276 PTRATRSPCLMSRLKLSRALMPSERYSTSTLRKLMRPSTRCGASVPLSCSGGVSRΝAHTP 190 200 210 220 230 240 250 260 270 279 m276.pep SAARNIVCIGMLMLARLLMGAYICSIATMNAINSPMVVX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g276 SAARNIVCIGMLMLARLLMGAYICSIATMNAINSPMWX 250 260 270 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1131>: a276 . seq 1 ATGATTTTGC CGTCGTCCAT TACGATGATG CGGTCGGCCC CTTCGATGGT 51 GGTCAGGCGG TGGGCGACGA TGATGCCGGT GCGGTTTTCC ATCAGGCGTT 101 CGAGCGCCTG TTGGACGAGG CGTTCGGATT CGTTGTCCAA TGCGCTGGTG 151 GCTTCGTCCA ATAATAATAT CGGCGCGTCT TTCAAAATGG CGCGGGCAAT 201 GGCAACGCGT TGCCGCTGTC CGCCGGATAA GTTGCTGCCG TTCGATCCGA 251 TGGGCTGGTG CAGTCCGAGC GGTGATGCGT CGATCAGGCT TTGCAGGTTA 301 GCGGCTTGGA GGGCGGATAG GACTTCGGCT TCGCCCGCGT CGGGACGGCT 351 ATATCGGACG TTTTCAAACA GGGTGTCGTC AAACAGGAAT ACGTCTTGGG 401 AGACGAGGGC AAATTGGGCG CGCAGGCAGT CGAGTTTGAT GTCGGCGATG 451 TCGATACCGT CTATGCAGAT GTTGCCGGCA GACGGTTCGA CAAAGCGGGG 501 CAGCAGGTTG ACGACGGTGG ATTTGCCGCT GCCGGAACGT CCGACCAGGG 551 CGACGCGTTC GCCTTGTCTG ATGTCGAGGT TGAAGCCGTC GAGGGCTTTG 601 ATGCCGTCCG AACGGTATTC GACATCGACG TTGCGGAAGC TGATGCGCCC 651 TTCGACACGC TGCGGTGCGA GCGTGCCTTT GTCCTGTTCG GGCGGGGTGT 701 CGAGAAATGC ACATACGCCG TCGGCGGCGA GGAACATCGT CTGCATAGGG 751 ATGCTAATGT TGGCAAGGCT TTTGATGGGG GCGTACATTT GCAGCATCGC 801 GACGATGAAT GCCATAAATT CGCCGATGGT GGTGTAG This corresponds to the amino acid sequence <SEQ ID 1132; ORF 276. a>: a276. pep 1 MILPSSITMM RSAPSMWRR WATMMPVRFS IRRSSACWTR RSDSLSNALV 51 ASSNNNIGAS FKMARAMATR CRCPPDKLLP FDPMGWCSPS GDASIRLCRL 101 AAWRADRTSA SPASGRLYRT FSNRVSSNRN TSWETRANWA RRQSSLMSAM 151 SIPSMQMLPA DGSTKRGSRL TTVDLPLPER PTRATRSPCL MSRLKPSRAL 201 MPSERYSTST LRKLMRPSTR CGASVPLSCS GGVSRNAHTP SAARNIVCIG 251 MLMLARLLMG AYICSIATMN AINSPMW* m276/a276 98.2% identity in 278 aa overlap 10 20 30 40 50 60 m276.pep MILPSSITMMRSAPSMVVRRWATMMPVRFSIRRSSACWTRRSDSLSNALVASSNNNIGAS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a276 MILPΞSITMMRSAPSMVVRRWATMMPVRFSIRRSΞACWTRRSDSLΞNALVASSNNNIGAS 10 20 30 40 50 60 70 80 90 100 110 120 m276. pep FKMARAMATRCRCPPDKLLPFDPMGWCSPSGELSIRLCRLAVWRANRTΞAΞPASGRLYRT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I h I I I I I I I h I I h I I I I I I I I I I I I I I a276 FKMARAMATRCRCPPDKLLPFDPMGWCSPΞGDASIRLCRLAAWRADRTSASPASGRLYRT 70 80 90 100 110 120 130 140 150 160 170 180 m276.pep FSNRVSSNRNTSWETRANWARRQSSLMSAMSIPSMQMLPADGSTKRGSRLTTVDLPLPER I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a276 FSNRVSΞNRNTSWETRANWARRQSSLMSAMSIPSMQMLPADGSTKRGSRLTTVDLPLPER 130 140 150 160 170 180 190 200 210 220 230 240 m276.pep PTRATRSPCLMSRLKLSRALMPSERYSTSTLRKLMRPSTRCGASVPLΞCSGGVSRNAHTP lllllllllllllll I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a276 PTRATRSPCLMSRLKPSRALMPSERYSTSTLRKLMRPSTRCGASVPLSCSGGVSRNAHTP 190 200 210 220 230 240 250 260 270 279 m276.pep SAARNIVCIGMLMLARLLMGAYICSIATMNAINSPMVVX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a276 SAARNIVCIGMLMLARLLMGAYICSIATMNAINSPMVVX 250 260 270 The following partial DNA sequence was identified in N.gonorrhoeae <SEQ ID 1133>: g277.seq (partial) 1 ..atggtacacg tcgccgtagc ttacggtatt gccgtccggc gtttttgccc 51 aaacgaggtc atagacgttt tccacgcctt gcaggtacat cgccaagcgt 101 tcgatgccgt aggtaatttc gccgagtacg ggcgtgcaat cgataccgcc 151 gacttgttgg aaataggtaa actgggttac ttccatgccg ttgagccaga 201 cttcccagcc caaaccccac gcaccgaggg tggggttttc ccagtcgtct 251 tcgacaaagc ggatgtcgtg gactttggga tcgatgccca attcgcgcag 301 ggagtcgaga tagaggtctt ggatattggc gggggcgggt ttgagggcga 351 cttggaattg gtaatagtgt tgcaggcggt tggggttgtc gccgtagcgg 401 ccgtctttgg ggcggcggct gggttggacg taggcggcaa accaaggctc 451 ggggccgagc gcgcgcaggc aggtggcggg atgggatgtg ccggcaccga 501 cttccatgtc gaagggttgg atgacggtgc agcctttgtc tgcccagaag 551 gtttgcagtt tgaagatgat ttgttggaag gtaagcatgg cttattgttc 601 gataaaataa aggttttatt ttactgtttc catagccgct tgaatagatt 651 tatctcgaag acagcctga This corresponds to the amino acid sequence <SEQ ID 1134; ORF 211. ng>: g277.pep (partial) 1 ..MVHVAVAYGI AVRRFCPNEV IDVFHALQVH RQAFDAVGNF AEYGRAIDTA 51 DLLEIGKLGY FHAVEPDFPA QTPRTEGGVF PWFDKADW DFGIDAQFAQ 101 GVEIEVLDIG GGGFEGDLEL VIVLQAVGW AVAAVFGAAA GLDVGGKPRL 151 GAERAQAGGG MGCAGTDFHV EGLDDGAAFV CPEGLQFEDD LLEGKHGLLF 201 DKIKVLFYCF HSRLNRFISK TA* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 1135>: m277.seq 1 ATGCCCCGCT TTGAGGACAA GCTCGTAGGC AGGCAGGGCG AGGGCGGCGT 51 TTTCTTCGGC AAGCAGGCGT TTGGCTTGCG CTTCGTAGTC GTTGAACTGG 101 CGCAGCAGCC AGTCGGCATC GCTGTATTCG AAGTTGTAGG TGGATTGCTC 151 GACTTCGTTT TGGTGGTACA CGTCGCCGTA GGTGACGGTG TTGCCGTCGA 201 GCGTTTTTGC CCAAACGAGG TCGTAGACGT TTTCTACACC TTGCAAGTAC 251 ATCGCCAAGC GTTCGATGCC GTAGGTGATT TCGCCGAGTA CGGGCGTGCA 301 GTCGATGCCG CCGACTTGTT GGAAATAGGT AAACTGGGTT ACTTCCATGC 351 CGTTGAGCCA GACTTCCCAG CCCAAACCCC ACGCGCCGAG GGTGGGGTTT 401 TCCCAGTCGT CTTCGACAAA GCGGATGTCG TGGACTTTGG GATCGATGCC 451 CAATTCGCGC AGAGAGTCGA GATAGAGGTC TTGGATATTG GCGGGAGCGG 501 GCTTGAGGGC GACTTGGAAT TGGTAATAGT GTTGCAGGCG GTTGGGGTTG 551 TCGCCGTAGC GGCCGTCTTT GGGGCGGCGG CTGGGTTGGA CGTAGGCGGC 601 AAACCAAGGC TCGGGGCCGA GTGCGCGCAG GCAGGTGGCG GGATGGGATG 651 TGCCGGCACC GACTTCCATG TCGAAGGGTT GGATGACGGT GCAGCCTTTG 701 TCTGCCCAGA ATGTTTGCAG TTTGAAGATG ATTTGTTGGA AGGTAAGCAT 751 GGCTTATGA This corresponds to the amino acid sequence <SEQ ID 1136; ORF 277>: m277.pep 1 MPRFEDKLVG RQGEGGVFFG KQAFGLRFW VELAQQPVGI AVFEWGGLL 51 DFVLWHVAV GDGVAVERFC PNEWDVFYT LQVHRQAFDA VGDFAEYGRA 101 VDAADLLEIG KLGYFHAVEP DFPAQTPRAE GGVFPWFDK ADWDFGIDA 151 QFAQRVEIEV LDIGGSGLEG DLELVIVLQA VGWAVAAVF GAAAGLDVGG 201 KPRLGAECAQ AGGGMGCAGT DFHVEGLDDG AAFVCPECLQ FEDDLLEGKH 251 GL* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 277 shows 90.0% identity over a 221 aa overlap with a predicted ORF (ORF 277.ng) from N gonorrhoeae: g277/m277 10 20 30 g277. pep MVHVAVAYGIAVRRFCPΝEVIDVFHALQVH : I I I II : I : I I : I I I I I I I : I I I :: I I I I m277 GLRFVWELAQQPVGIAVFEWGGLLDFVLWHVAVGDGVAVERFCPNEVVDVFYTLQVH 30 40 50 60 70 80 40 50 60 70 80 90 g277. pep RQAFDAVGNFAEYGRAIDTADLLEIGKLGYFHAVEPDFPAQTPRTEGGVFPWFDKADW I I I I I I I I : I I I I I I I : I : I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I m277 RQAFDAVGDFAEYGRAVDAADLLEIGKLGYFHAVEPDFPAQTPRAEGGVFPWFDKADW 90 100 110 120 130 140 100 110 120 130 140 150 g277. pep DFGIDAQFAQGVEIEVLDIGGGGFEGDLELVIVLQAVGWAVAAVFGAAAGLDVGGKPRL I I I I I I I I I I I I I I I I I I I I : I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m277 DFGIDAQFAQRVEIEVLDIGGSGLEGDLELVIVLQAVGWAVAAVFGAAAGLDVGGKPRL 150 160 170 180 190 200 160 170 180 190 200 g277.pep GAERAQAGGGMGCAGTDFHVEGLDDGAAFVCPEGLQFEDDLLEGKHGLL III I I I I I I I I I I I I I I I I I I I I I I I I I I I I I llllllllllllll m277 GAECAQAGGGMGCAGTDFHVEGLDDGAAFVCPECLQFEDDLLEGKHGLX 210 220 230 240 250 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1137>: a277 . seq 1 ATGCCCCGCT TTGAGGACAA GCTCGTAGGC AGGCAGGGCG AGGGCGGCGT 51 TTTCTTCGGC AAGCAGGCGT TTGGCTTGCG CTTCGTAGTC GTTGAACTGG 101 CGCAGCAGCC AATCGGCATC GCTGTATTCG AAGTTGTAGG TGGATTGTTC 151 GACTTCGTTT TGGTGGTACA CGTCGCCGTA AGTTACTGTA TTACCGTCCA 201 GCGTTTTTGC CCAAACGAGG TCATAGACGT TTTCCACGCC TTGCAGGTAC 251 ATCGCCAAGC GTTCGATGCC GTAGGTGATT TCGCCGAGTA CGGGGGTGCA 301 GTCGATGCCG CCGACTTGTT GGAAATAGGT GAACTGGGTT ACTTCCATAC 351 CGTTGAGCCA GACTTCCCAG CCCAAACCCC ACGCGCCGAG GGTGGGGTTT 401 TCCCAGTCGT CTTCGACAAA GCGGATGTCG TGCACTTTGG GGTCGATGCC 451 CAATTCGCGC AGGGAGTCGA GATAGAGGTC TTGGATATTG GCGGGAGCGG 501 GCTTGAGGGC GACTTGGAAT TGGTAATAGT GTTGCAGGCG GTTGGGGTTG 551 TCGCCGTAGC GACCGTCTTT GGGGCGGCGG CTGGGTTGGA CGTAGGCGGC 601 AAACCAAGGC TCGGGGCCGA GTGCGCGCAG ACAGGTGGCG GGATGGGATG 651 TGCCGGCACC GACTTCCATG TCGAAGGGTT GGATGACGGT GCAGCCTTTG 701 TCTGCCCAGA ATGTTTGCAG TTTGAAGATG ATTTGTTGGA AGGTAAGCAT 751 GGCTTATGA This corresponds to the amino acid sequence <SEQ ID 1138; ORF 277. a>: a277.pep 1 MPRFEDKLVG RQGEGGVFFG KQAFGLRFVV VELAQQPIGI AVFEWGGLF 51 DFVLWHVAV SYCITVQRFC PΝEVIDVFHA LQVHRQAFDA VGDFAEYGGA 101 VDAADLLEIG ELGYFHTVEP DFPAQTPRAE GGVFPWFDK ADWHFGVDA 151 QFAQGVEIEV LDIGGSGLEG DLELVIVLQA VGWAVATVF GAAAGLDVGG 201 KPRLGAECAQ TGGGMGCAGT DFHVEGLDDG AAFVCPECLQ FEDDLLEGKH 251 GL* m277/a277 92.5% identity in 252 aa overlap 10 20 30 40 50 60 m277. pep MPRFEDKLVGRQGEGGVFFGKQAFGLRFVVVELAQQPVGIAVFEVVGGLLDFVLVVHVAV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I : I I I I I I I I I I a277 MPRFEDKLVGRQGEGGVFFGKQAFGLRFVVVELAQQPIGIAVFEVVGGLFDFVLVVHVAV 10 20 30 40 50 60 70 80 90 100 110 120 m277.pep GDGVAVERFCPΝEVVDVFYTLQVHRQAFDAVGDFAEYGRAVDAADLLEIGKLGYFHAVEP : :: h I I I I I I I :l I h: I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I : I I I a277 SYCITVQRFCPΝEVIDVFHALQVHRQAFDAVGDFAEYGGAVDAADLLEIGELGYFHTVEP 70 80 90 100 110 120 130 140 150 160 170 180 m277 . pep DFPAQTPRAEGGVFPVVFDKADVVDFGIDAQFAQRVEIEVLDIGGSGLEGDLELVIVLQA I I I I I I I I I I I I I I I I I I I I I I I I I h I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a277 DFPAQTPRAEGGVFPVVFDKADVVHFGVDAQFAQGVEIEVLDIGGSGLEGDLELVIVLQA 130 140 150 160 170 180 190 200 210 220 230 240 m277. pep VGVVAVAAVFGAAAGLDVGGKPRLGAECAQAGGGMGCAGTDFHVEGLDDGAAFVCPECLQ I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I :| I I I I I I I I I I I I I I I I I I I I I I I I I I I I a277 VGVVAVATVFGAAAGLDVGGKPRLGAECAQTGGGMGCAGTDFHVEGLDDGAAFVCPECLQ 190 200 210 220 230 240 250 m277. pep FEDDLLEGKHGLX I I I I I I I I I I I I I a277 FEDDLLEGKHGLX 250 The following partial DNA sequence was identified in N.gonorrhoeae <SEQ ID 1139>: g278.seq (partial) 1 ttgcgtgcaa tcacgcccgg tgcgattttt tcgacagggg cggtcaaagt 51 tgtattaatc ggacctttgc cgtcgatagg ccgacccaat gcatcgacga 101 cgcgtccgac caattcgcgt ccgaccggca cttctaaaat acggccggta 151 caggtaaccg tgtcgccttc tttaatatgt tcgtactcgc ccaacactac 201 ggcaccgacg gagtcgcgct ccaggttcat cgccaagcct aaagtgttac 251 ccgggaattc gagcatctca ccttgcattg catctgacaa accatggatg 301 cgaacgatac cgtcagttac cgaaatcacc gtaccacggg tactcacttc 351 ggcatttaca gacagatttt cgatcttggc tttaatcaga tcgctaattt 401 cagcaggatt aagctgcatg aaaactctcc taattcgtca tagtcgtgta 451 caaagcactc agtttgcctt gtacagacaa atccaaaacc tgatcaccca 501 cttcaacttt ta... This corresponds to the amino acid sequence <SEQ ID 1140; ORF 278.ng>: g278.pep (partial) 1 LRAITPGAIF STGAVKWLI GPLPSIGRPN ASTTRPTNSR PTGTSKIRPV 51 QVTVSPSLIC SYSPNTTAPT ESRSRFIAKP KVLPGNSSIS PCIASDKPWM 101 RTIPSVTEIT VPRVLTSAFT DRFSILALIR SLISAGLSCM KTLLIRHSRV 151 QSTQFALYRQ IQNLITHFNF .... The following partial DNA sequence was identified in N. meningitidis <SEQ ID 1141>: m278.seq.. 1 TTGCGCGCAA TCACGCCCGG TGCGATTTTT TCGATAGGGG CGGTCAAAGT 51 TGTATTAATC GGGCCTTTGC CGTCGATAGG CCGACCCAAT GCATCAACGA 101 CGCGTCCGAC CAGTTCGCGT CCGACCGGCA CTTCCAAGAT ACGACCGGTA 151 CAGGTAACCG TGTCGCCTTC TTTAATGTGT TCGTACTCGC CCAACACTAC 201 GGCGCCGACG GAGTCGCGCT CCAGGTTCAT CGCCAAGCCG AAAGTGTTAC 251 CCGGGAATTC GAGCATCTCA CCTTGCATTG CATCTGACAA ACCATGGATG 301 CGAACGATAC CGTCAGTTAC CGAAATTACC GTACCACAGG TACGCACTTC 351 GGCATTTACA GACAGATTTT CGATCTTGGC TTTAATCAAA TCGCTAATTT 401 CAGCAGGATT AAGCTGCATG AAAACTCTCC TAATTCGTCA TAGTCGTGTA 451 CAAGGCACTC AATTTGCCTT GTACAGACAA ATCCAAAACC TGATCACCCA 501 CTTCAACTTT TATGCCGCCA ATCAGCTCCG GTTCGATTTC GACAGAGATT 551 TTCAGCTCGC TGTCGAAACG CTTATTCAGC ATTTGCACCA ACTCGCCGAC 601 CTGTTTGTCG GTCAACGGAT AGGCACTGTA AATGACGGCA GATTTGATAT 651 GGTTGAATGA This corresponds to the amino acid sequence <SEQ ID 1142; ORF 278>: m278.pep 1 LRAITPGAIF SIGAVKWLI GPLPSIGRPN ASTTRPTSSR PTGTSKIRPV 51 QVTVSPSLMC SYSPNTTAPT ESRSRFIAKP KVLPGNSSIS PCIASDKPWM 101 RTIPSVTEIT VPQVRTSAFT DRFSILALIK SLISAGLSCM KTLLIRHSRV 151 QGTQFALYRQ IQNLITHFNF YAANQLRFDF DRDFQLAVET LIQHLHQLAD 201 LFVGQRIGTV NDGRFDMVE* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 278 shows 95.9% identity over a 170 aa overlap with a predicted ORF (ORF 278. ng) from N gonorrhoeae: g278/m278 10 20 30 40 50 60 g278.pep LRAITPGAIFSTGAVKWLIGPLPSIGRPΝASTTRPTΝSRPTGTSKIRPVQVTVSPSLIC I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I : I m278 LRAITPGAIFSIGAVKWLIGPLPΞIGRPΝASTTRPTSSRPTGTSKIRPVQVTVSPSLMC 10 20 30 40 50 60 70 80 90 100 110 120 g278.pep SYSPΝTTAPTESRSRFIAKPKVLPGΝSSISPCIASDKPWMRTIPSVTEITVPRVLTSAFT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I m278 SYSPΝTTAPTESRSRFIAKPKVLPGΝSSISPCIASDKPWMRTIPSVTEITVPQVRTSAFT 70 80 90 100 110 120 130 140 150 160 170 g278.pep DRFSILALIRSLISAGLSCMKTLLIRHSRVQSTQFALYRQIQΝLITHFΝF I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I m278 DRFSILALIKSLISAGLSCMKTLLIRHSRVQGTQFALYRQIQΝLITHFΝFYAAΝQLRFDF 130 140 150 160 170 180 m278 DRDFQLAVETLIQHLHQLADLFVGQRIGTVΝDGRFDMVE* 190 200 210 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1143>: a278.seq 1 TTGCGCGCAA TCACGCCCGG TGCGATTTTT TCGATAGGGG CGGTCAAAGT 51 TGTATTAATC GGGCCTTTGC CGTCGATAGG CCGACCCAAT GCATCAACGA 101 CGCGTCCGAC CAGTTCGCGT CCGACCGGCA CTTCCAAGAT ACGACCGGTA 151 CAGGTAACCG TGTCGCCTTC TTTAATATGT TCGTGCTCGC CCAACACTAC 201 GGCGCCGACG GAGTCGCGCT CCAGGTTCAT CGCCAAGCCG AAAGTGTTAC 251 CCGGGAATTC GAGCATCTCA CCTTGCATTG CATCTGACAA ACCATGGATG 301 CGAACGATAC CGTCAGTTAC CGAAATCACC GTACCACGGG TACGCACTTC 351 GGCATTTACA GACAGATTTT CGATCTTGGC TTTAATCAAA TCGCTAATTT 401 CAGCAGGATT AAGCTGCATG AAAACTCTCC TAATTCGTCA TAGTCGTGTA 451 CAAGGCACTC AATTTGCCTT GTACAGACAA ATCCAAAACC TGATCACCCA 501 CTTCAACTTT TATGCCGCCA ATCAGCTCCG GTTCGATTTC GACAGAGATT 551 TTCAGCTCGC TGTCGAAACG CTTATTCAGC ATTTGCGCCA ACTCGCCGAC 601 CTGTTTGTCG GTCAACGGAT AGGCACTGTA AATGACGGCA GATTTGATAT 651 GGTTGAATGA This corresponds to the amino acid sequence <SEQ ID 1144; ORF 278. a>: a278.pep 1 LRAITPGAIF SIGAVKWLI GPLPSIGRPN ASTTRPTSSR PTGTSKIRPV 51 QVTVSPSLIC SCSPΝTTAPT ESRSRFIAKP KVLPGNSSIS PCIASDKPWM 101 RTIPSVTEIT VPRVRTSAFT DRFSILALIK SLISAGLSCM KTLLIRHSRV 151 QGTQFALYRQ IQNLITHFNF YAANQLRFDF DRDFQLAVET LIQHLRQLAD 201 LFVGQRIGTV NDGRFDMVE* m278/a278 98.2% identity in 219 aa overlap 10 20 30 40 50 60 m278. .pep LRAITPGAIFSIGAVKVVLIGPLPSIGRPNASTTRPTSSRPTGTSKIRPVQVTVSPSLMC I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I hi a278 LRAITPGAIFSIGAVKVVLIGPLPSIGRPNASTTRPTSSRPTGTSKIRPVQVTVSPSLIC 10 20 30 40 50 60 70 80 90 100 110 120 m278. ■pep SYSPNTTAPTESRSRFIAKPKVLPGNΞΞISPCIASDKPWMRTIPSVTEITVPQVRTΞAFT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I |: I I M I I I a278 SCSPNTTAPTESRSRFIAKPKVLPGNSSISPCIASDKPWMRTIPSVTEITVPRVRTSAFT 70 80 90 100 110 120 130 140 150 160 170 180 m278.pep DRFSILALIKSLISAGLSCMKTLLIRHSRVQGTQFALYRQIQNLITHFNFYAANQLRFDF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a278 DRFSILALIKSLISAGLSCMKTLLIRHSRVQGTQFALYRQIQNLITHFNFYAANQLRFDF 130 140 150 160 170 180 190 200 210 220 m278.pep DRDFQLAVETLIQHLHQLADLFVGQRIGTVNDGRFDMVEX I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I a278 DRDFQLAVETLIQHLRQLADLFVGQRIGTVNDGRFDMVEX 190 200 210 220 The following partial DNA sequence was identified in N.gonorrhoeae <SEQ ID 1145>: g279.seq 1 atgacgcgga tttgcggctg cttgatttca acggttttga gtgtttcggc 51 aagtttgtcg gcggcgggtt tcatcaggct gcaatgggaa ggaacggata 101 ccggcagcgg cagggcgcgt ttggctccgg cttctttggc ggcagccatg 151 gtgcgtccga cggcggcggc gttgcctgca atcacgactt gtccgggcga 201 gttgaagttg acggcttcga ccacttcgcc ctgtgcggat tcggcacaaa 251 tctgcctgac ctgttcatct tccaaaccca aaatggccgc cattgcgcct 301 acgccttgcg gtacggcgga ctgcatcagt tcggcgcgca ggcggacgag 351 tttgacggca tcggcaaaat ccaatgcttc ggcggcgaca agcgcggtgt 401 attcgccgag gctgtgtccg gcaacggcgg caggcgtttt gccgcccact 451 tccaaatag This corresponds to the amino acid sequence <SEQ ID 1146; ORF 279.ng>: g279.pep 1 MTRICGCLIS TVLSVSASLS AAGFIRLQWE GTDTGSGRAR LAPASLAAAM 51 VRPTAAALPA ITTCPGELKL TASTTSPCAD SAQICLTCSS SKPKMAAIAP 101 TPCGTADCIS SARRRTSLTA SAKSNASAAT SAVYSPRLCP ATAAGVLPPT 151 SK* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 1147>: m279.seq 1 ATAACGCGGA TTTGCGGCTG CTTGATTTCA ACGGTTTTCA GGGCTTCGGC 51 AAGTTTGTCG GCGGCGGGTT TCATCAGGCT GCAATGGGAA GGTACGGACA 101 CGGGCAGCGG CAGGGCGCGT TTGGCACCGG CTTCTTTGGC GGCAGCCATG 151 GCGCGTCCGA CGGCGGCGGC GTTGCCTGCA ATCACGATTT GTCCGGGTGA 201 GTTGAAGTTG ACGGCTTCGA CCACTTCGCT TTGGGCGGCT TCGGCACAAA 251 TGGCTTTAAC CTGCTCATCT TCCAAGCCGA GAATCGCCGC CATTGCGCCC 301 ACGCCTTGCG GTACGGCGGA CTGCATCAGT TCGGCGCGCA GGCGCACGAG 351 TTTGACCGCG TCGGCAAAAT TCAATGCGCC GGCGGCAACG AGTGCGGTGT 401 ATTCGCCGAG GCTGTGTCCG GCAACGGCGG CAGGCGTTTT GCCGCCCGCT 451 TCTAAATAG This corresponds to the amino acid sequence <SEQ ID 1148; ORF 279>: m279.pep 1 ITRICGCLIS TVFRASASLS AAGFIRLQWE GTDTGSGRAR LAPASLAAAM 51 ARPTAAALPA ITICPGELKL TASTTSLWAA SAQMALTCSS SKPRIAAIAP 101 TPCGTADCIS SARRRTSLTA SAKFNAPAAT SAVYSPRLCP ATAAGVLPPA 151 SK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 279 shows 89.5% identity over a 152 aa overlap with a predicted ORF (ORF 279.ng) from N gonorrhoeae: 10 20 30 40 50 60 m279.pep ITRICGCLISTVFRASASLSAAGFIRLQWEGTDTGSGRARLAPASLAAAMARPTAAALPA = 11111111111= g279 MTRICGCLISTVLSVSASLSAAGFIRLQWEGTDTGSGRARLAPASLAAAMVRPTAAALPA 10 20 30 40 50 60 70 80 90 100 110 120 m279 . pep ITICPGELKLTASTTSLWAASAQMALTCSSSKPRIAAIAPTPCGTADCISSARRRTSLTA I I M M M I M M M I 1 1 1 = 1 1 1 1 1 I I I = = I I 1 1 1 1 I I I I I I I I I I I I I 1 1 1 1 I I g279 ITTCPGELKLTASTTSPCADSAQICLTCSSSKPKMAAIAPTPCGTADCISSARRRTSLTA 70 80 90 100 110 120 130 140 150 m279. pep SAKFNAPAATSAVYSPRLCPATAAGVLPPASKX I I I I I g279 SAKSNASAATSAVYSPRLCPATAAGVLPPTSKX 130 140 150 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1149>: a279.seq 1 ATGACΝCΝGA TTTGCGGCTG CTTGATTTCA ACGGTTTΝΝA GGGCTTCGGC 51 GAGTTTGTCG GCGGCGGGTT TCATGAGGCT GCAATGGGAA GGTACΝGACA 101 CΝGGCAGCGG CAGGGCGCGT TTGGCGCCGG CTTCTTTGGC GGCAAGCATA 151 GCGCGCTCGA CGGCGGCGGC ATTGCCTGCA ATCACGACTT GTCCGGGCGA 201 GTTGAAGTTG ACGGCTTCAA CCACTTCATC CTGTGCGGAT TCGGCGCAAA 251 TTTGTTTTAC CTGTTCATCT TCCAAGCCGA GAATCGCCGC CATTGCGCCC 301 ACGCCTTGCG GTACGGCGGA CTGCATCAGT TCGGCGCGCA ΝGCGCACGAG 351 TTTGACCGCG TCGGCAAAAT CCAATGCGCC GGCGGCAACΝ AGTGCGGTGT 401 ATTCGCCGAΝ GCTGTGTCCG GCAACGGCGG CAGGCGTTTT GCCGCCCGCT 451 TCCGAATAG This corresponds to the amino acid sequence <SEQ ID 1150; ORF 279. a>: a279.pep 1 MTXICGCLIS TVXRASASLS AAGFMRLQWE GTDTGSGRAR LAPASLAASI 51 ARSTAAALPA ITTCPGELKL TASTTSSCAD SAQICFTCSS SKPRIAAIAP 101 TPCGTADCIS SARXRTSLTA SAKSΝAPAAT SAVYSPXLCP ATAAGVLPPA 151 SE* m279/a279 88.2% identity in 152 aa overlap 10 20 30 40 50 60 m279.pep ITRICGCLISTVFRASASLSAAGFIRLQWEGTDTGSGRARLAPASLAAAMARPTAAALPA : I lllllllll I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I :: I I lllllll a279 MTXICGCLISTVXRASASLSAAGFMRLQWEGTDTGSGRARLAPASLAASIARSTAAALPA 10 20 30 40 50 60 70 80 90 100 110 120 m279. pep ITICPGELKLTASTTSLWAASAQMALTCSSSKPRIAAIAPTPCGTADCISSARRRTSLTA II I I I I I I I I I I I I I I ll : I I I I I I I I I I I I I I I I I I I I I I I I I I I llllll a279 ITTCPGELKLTASTTSSCADSAQICFTCSSSKPRIAAIAPTPCGTADCISSARXRTSLTA 70 80 90 100 110 120 130 140 150 m279. pep SAKFΝAPAATSAVYSPRLCPATAAGVLPPASKX III I I I I I I I I I I I I I I I I I I I I I I I I I h I a279 SAKSΝAPAATSAVYSPXLCPATAAGVLPPASEX 130 140 150 Expression of ORF 279 The primer described in Table 1 for ORF 279 was used to locate and clone ORF 279. ORF 279 was cloned in pET and pGex vectors and expressed in E.coli as above-described. The products of protein expression and purification were analyzed by SDS-PAGE. Figure 2A shows the results of affinity purification and Figure 2B shows the expression in E.coli. Purified GST-fusion protein was used to immunize mice whose sera were used for ELISA (positive result), FACS analysis (Figure 2C), western blot (Figure 2D). These experiments confirm that 279 is a surface-exposed protein and that it is a useful immunogen. The hydrophilicity plots, antigenic index, and amphipatic regions of ORF 279 are provided in Figure 6. The AMPHI program is used to predict putative T-cell epitopes (Gao et al 1989, J. Immunol 143:3007; Roberts et al. 1996, AIDS Res Human Retroviruses 12:593; Quakyi et al. 1992, Scand J Immunol Suppl 11:9). The nucleic acid sequence of ORF 279 and the amino acid sequence encoded thereby is provided herein. The following partial DNA sequence was identified in N.gonorrhoeae <SEQ ID 1151>: g280.seq 1 atgaaacacc tcaaacttac ccttattgcc gcattgctgg ccaccgccgc 51 aactgccgca ccccttccgg ttgtaaccag tttcagcatt ttaggcgacg 101 tagccaaaca aatcggcggt gagcgcgtag ccgtacaaag cctcgtcgga 151 gccaaccaag atactcatgc ctatcacatg accagtggcg acattaaaaa 201 aatccgcagt gcaaaactcg tcctgctcaa cggcttggga cttgaagccg 251 ccgacatcca acgcgccgtc aaacagagca aagtatccta tgccgaagcg 301 accaaaggca tccaacccct caaagccgaa gaagaaggcg gacaccatca 351 cgaccaccat cacgaccacg atcatgacca cgaaggacac caccacgacc 401 acggcgaata tgacccccac gtctggaacg accctgttct tatgtccgac 451 tatgcccaaa acgtcgctga aaccctgata aaggccgatc ccgaaggcaa 501 agtttattat caacaacgct tgggcaacta ccaaatgcag cttaaaaaac 551 tgcacagcga cgcacaagcc gcatttaatg ccgtccctgc cgccaaacgc 601 aaagtcctga ccgggcacga cgcattttcc tacatgggca accgctacaa 651 catcagcttc atcgccccgc aaggcgtgag cagcgaagcc gagccgtccg 701 ccaaacaagt cgccgccatc atccggcaaa tcaaacgcga aggcatcaaa 751 gccgtattta ccgaaaatat caaagacacc cgcatggttg accgcatcgc 801 caaagaaacc ggcgtcaacg tcagcggcaa actgtattcc gacgcactcg 851 gcaacgcgcc cgcagacacc tacatcggca tgtaccgcca caacgtcgaa 901 gccttgacca acgcgatgaa gcaataa This corresponds to the amino acid sequence <SEQ ID 1152; ORF 280.ng>: g280.pep 1 MKHLKLTLIA ALLATAATAA PLPWTSFSI LGDVAKQIGG ERVAVQSLVG 51 ANQDTHAYHM TSGDIKKIRS AKLVLLNGLG LEAADIQRAV KQSKVSYAEA 101 TKGIQPLKAE EEGGHHHDHH HDHDHDHEGH HHDHGEYDPH VWNDPVLMSD 151 YAQNVAETLI KADPEGKVYY QQRLGNYQMQ LKKLHSDAQA AFNAVPAAKR 201 KVLTGHDAFS YMGNRYNISF IAPQGVSSEA EPSAKQVAAI IRQIKREGIK 251 AVFTENIKDT RMVDRIAKET GVNVSGKLYS DALGNAPADT YIGMYRHNVE 301 ALTNAMKQ* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 1153>: m280.seq 1 ATGAAACACC TCAAACTCAC CCTTATTGCC GCATTGCTGA CCGCCTCCGC 51 AACTGCCGCC CCCCTGCCGG TTGTAACCAG CTTCAGCATT TTAGGCGACG 101 TAGCCAAACA AATCGGCGGA GAGCGCGTAT CCATACAAAG TTTGGTCGGA 151 GCCAACCAAG ATACGCACGC CTATCATATG ACCAGTGGCG ACATTAAAAA 201 AATCCGCAGT GCAAAACTCG TCCTGCTCAA CGGCTTAGGA CTTGAAGCTG 251 CCGATGTGCA ACGCGCCGTC AAACAAAGCA AAGTATCCTA TACCGAAGCG 301 ACCAAAGGCA TCCAACCCCT CAAAGCCGAA GAAGAAGGCG GACACCATCA 351 CGACCACGAT CATGACCACG AAGGACACCA CCATGACCAC GGCGAATATG 401 ACCCGCACGT CTGGAACGAC CCCGTCCTTA TGTCCGCCTA TGCCCAAAAC 451 GTTGCCAAAG CCCTGATAAA GGCCGATCCC GAAGGCAAAG TTTATTATCA 501 ACAACGCTTG GGCAACTACC AAATGCAGCT CAAAAAACTG CACAGCGACG 551 CACAAGCCGC ATTTAATGCC GTCCCTGCTG CCAAACGCAA AGTCCTGACC 601 GGGCACGATG CCTTTTCCTA TATGGGCAAA CGTTACCATA TCGAATTCAT 651 CGCCCCGCAA GGCGTGAGCA GCGAAGCCGA GCCTTCGGCC AAACAAGTCG 701 CCGCCATCAT CCGACAAATC AAACGCGAAG GCATCAAAGC CGTCTTTACC 751 GAAAACATCA AGGACACCCG TATGGTTGAC CGTATCGCCA AAGAAACCGG 801 TGTCAACGTC AGCGGCAAAC TGTATTCCGA CGCACTCGGC AACGCGCCCG 851 CAGACACCTA CATCGGAATG TACCGCCACA ACATCAAAGC CTTGACCAAC 901 GCGATGAAGC AATAA This corresponds to the amino acid sequence <SEQ ID 1154; ORF 280>: m280.pep 1 MKHLKLTLIA ALLTASATAA PLPWTSFSI LGDVAKQIGG ERVSIQSLVG 51 ANQDTHAYHM TSGDIKKIRS AKLVLLNGLG LEAADVQRAV KQSKVSYTEA 101 TKGIQPLKAE EEGGHHHDHD HDHEGHHHDH GEYDPHVWND PVLMSAYAQN 151 VAKALIKADP EGKVYYQQRL GNYQMQLKKL HSDAQAAFNA VPAAKRKVLT 201 GHDAFSYMGK RYHIEFIAPQ GVSSEAEPSA KQVAAIIRQI KREGIKAVFT 251 ENIKDTRMVD RIAKETGVNV SGKLYSDALG NAPADTYIGM YRHNIKALTN 301 AMKQ* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 280 shows 93.8% identity over a 308 aa overlap with a predicted ORF (ORF 280.ng) from N gonorrhoeae: m280/g280 10 20 30 40 50 60 m280.pep MKHLKLTLIAALLTASATAAPLPWTSFSILGDVAKQIGGERVSIQSLVGAΝQDTHAYHM M I M M M M M = = = I M M M M M M I I M M M M M I h = I M M M M M M M g280 MKHLKLTLIAALLATAATAAPLPWTSFSILGDVAKQIGGERVAVQSLVGAΝQDTHAYHM 10 20 30 40 50 60 70 80 90 100 110 119 m280. pep TSGDIKKIRSAKLVLLΝGLGLEAADVQRAVKQSKVSYTEATKGIQPLKAEEEGGHHHDH- g280 TSGDIKKIRSAKLVLLΝGLGLEAADIQRAVKQSKVSYAEATKGIQPLKAEEEGGHHHDHH 70 80 90 100 110 120 120 130 140 150 160 170 m280. pep DHDHEGHHHDHGEYDPHVWΝDPVLMSAYAQΝVAKALIKADPEGKVYYQQRLGΝYQMQ 1111 II = = 11 II 11111111 II I 1111111 g280 HDHDHDHEGHHHDHGEYDPHVWΝDPVLMSDYAQΝVAETLIKADPEGKVYYQQRLGΝYQMQ 130 140 150 160 170 180 180 190 200 210 220 230 m280. pep LKKLHSDAQAAFΝAVPAAKRKVLTGHDAFSYMGKRYHIEFIAPQGVSSEAEPSAKQVAAI M M M M M M M M M M M M I M M M I h l h l I I I 1 1 1 1 1 I I I I I I I 1 1 I I 1 1 g280 LKKLHSDAQAAFΝAVPAAKRKVLTGHDAFSYMGΝRYΝISFIAPQGVSSEAEPSAKQVAAI 190 200 210 220 230 240 240 250 260 270 280 290 m280.pep IRQIKREGIKAVFTEΝIKDTRMVDRIAKETGVΝVSGKLYSDALGΝAPADTYIGMYRHΝIK MMMMMMMMMMMMMMMMMMMMMMMMMMMMIh: g280 IRQIKREGIKAVFTEΝIKDTRMVDRIAKETGVΝVSGKLYSDALGΝAPADTYIGMYRHΝVE 250 260 270 280 290 300 300 m280. pep ALTΝAMKQX g280 ALTΝAMKQX The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 1155>: a280.seq 1 ATGAAACACC CCAAACTCAC CCTTATCGCC GCATTGCTGA CCACTGCCGC 51 AACTGCCGCC CCCCTGCCGG TTGTAACCAG CTTCAGCATT TTAGGCGACG 101 TAGCCAAACA AATCGGCGGA GAGCGCGTAT CCATACAAAG TTTGGTCGGA 151 GCCAACCAAG ATACGCACGC CTATCATATG ACCAGCGGCG ACATTAAAAA 201 AATCCGCAGT GCAAAACTCG TCCTGATTAA CGGCTTAGGA CTTGAAGCTG 251 CCGACATCCA ACGTGCCGTC AAACAGAGCA AAGTATCCTA TGCCGAAGCG 301 ACCAAAGGCA TCCAACCCCT CAAAGCCGAA GAAGAAGGCG GACACCATCA 351 CGACCACGAT CATGACCACG ACCATGACCA CGAAGGACAC CACCACGACC 401 ACGGCGAATA TGACCCCCAC GTCTGGAACG ACCCCGTCCT TATGTCCGCC 451 TATGCCCAAA ACGTCGCCGA AGCCCTGATA AAGGCCGACC CCGAAGGCAA 501 AGTTTATTAT CAACAACGCT TGGGCAACTA CCAAATGCAG CTCAAAAAAC 551 TGCACAGTGA CGCACAAGCC GCATTTAATG CCGTCCCTGC CGCCAAACGC 601 AAAGTCCTGA CCGGGCACGA TGCCTTTTCC TATATGGGCA AACGTTACCA 651 TATCGAATTC ATCGCCCCAC AAGGTGTGAG CAGCGAAGCC GAGCCTTCAG 701 CCAAACAAGT CGCCGCCATC ATCCGACAAA TCAAACGCGA AGGCATCAAA 751 GCCGTATTTA CCGAAAATAT CAAAGACACC CGCATGGTTG ACCGCATCGC 801 CAAAGAAACC GGTGTCAACG TCAGCGGCAA ACTGTATTCC GACGCACTCG 851 GCAACGCACC CGCAGACACC TACATCGGCA TGTACCGCCA CAACATCAAA 901 GCCTTAACCA ACGCGATGAA GCAATAA This corresponds to the amino acid sequence <SEQ ID 1156; ORF 280.a>: a280.pep 1 MKHPKLTLIA ALLTTAATAA PLPWTSFSI LGDVAKQIGG ERVSIQSLVG 51 ANQDTHAYHM TSGDIKKIRS AKLVLINGLG LEAADIQRAV KQSKVSYAEA 101 TKGIQPLKAE EEGGHHHDHD HDHDHDHEGH HHDHGEYDPH VWNDPVLMSA 151 YAQNVAEALI KADPEGKVYY QQRLGNYQMQ LKKLHSDAQA AFNAVPAAKR 201 KVLTGHDAFS YMGKRYHIEF IAPQGVSSEA EPSAKQVAAI IRQIKREGIK 251 AVFTENIKDT RMVDRIAKET GVNVSGKLYS DALGNAPADT YIGMYRHNIK 301 ALTNAMKQ* m280/a280 96.4% identity in 308 aa overlap 10 20 30 40 50 60 m280. pep MKHLKLTLIAALLTASATAAPLPWTSFSILGDVAKQIGGERVSIQSLVGANQDTHAYHM III I I I I I I I I I I : : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a280 MKHPKLTLIAALLTTAATAAPLPWTSFSILGDVAKQIGGERVSIQSLVGANQDTHAYHM 10 20 30 40 50 60 70 80 90 100 110 120 m280. pep TSGDIKKIRSAKLVLLNGLGLEAADVQRAVKQSKVSYTEATKGIQPLKAEEEGGHHHDHD I I I I I I I I I I I I I I I : I I I I I I I I h I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I a280 TSGDIKKIRSAKLVLINGLGLEAADIQRAVKQSKVSYAEATKGIQPLKAEEEGGHHHDHD 70 80 90 100 110 120 130 140 150 160 170 m280. pep HDH EGHHHDHGEYDPHVWNDPVLMSAYAQNVAKALIKADPEGKVYYQQRLGNYQMQ III I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I a280 HDHDHDHEGHHHDHGEYDPHVWNDPVLMSAYAQNVAEALIKADPEGKVYYQQRLGNYQMQ 130 140 150 160 170 180 180 190 200 210 220 230 m280. pep LKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHIEFIAPQGVSSEAEPSAKQVAAI I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a280 LKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHIEFIAPQGVSSEAEPSAKQVAAI 190 200 210 220 230 240 240 250 260 270 280 290 m280. pep IRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYSDALGNAPADTYIGMYRHNIK I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a280 IRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYSDALGNAPADTYIGMYRHNIK 250 260 270 280 290 300 300 m280. pep ALTNAMKQX a280 ALTNAMKQX The following partial DNA sequence was identified in N.gonorrhoeae <SEQ ID 1157>: g281. seq 1 atgcactacg ccctcgcatc cgtcttctgc ctgtccctca gcgccgcacc 51 cgtcggcgta ttcctcgtca tgcgccgtat gagcctgata ggcgacgcat 101 tgagccacgc cgtcctgccc ggtgccgccg tcggctacat gtttgccggc 151 ttgagcctgc ccgctatggg tgtgggcggg tttgccgccg gtatgctgat 201 ggcgctgctt gccggactcg tcagccgctt taccaccctg aaagaagatg 251 ccaactttgc cgccttttac ctgagcagcc tcgccatcgg cgtaatcctc 301 atcagcaaaa acggcagcag cgtcgattta ctccacctcc ttttcggatc 351 tgtgcttgcc gtcgatattc ccgcactgca actcatcgcc gccgtctccg 401 gcctcacgct cattaccctt gccgtcatct accgccccct ggtgctagaa 451 agcatagacc cccttttcct caagtccgtc aacggcaaag gcgggctttg 501 gcacgtcatt ttcctcatcc tcgtcgttat gaacctcgta tccggcttcc 551 aagctctcgg catcctgatg tcggtcggaa ttatgatgct gcccgccatt 601 accgcccgtt tatgggcaag aaatatgggg acgctcattc tgttgtccgt 651 cctcatcgcc cttttttgcg gtttgatcgg gctgctcatt tcctaccaca 701 tcgaaatccc ttccggcccc gccatcatcc tctgttgcag cgtcctttat 751 cttttttccg tcatactcgg caaagaaggc ggcatcttgc ccaaatggtt 801 caaaaaccac cgccaccaca ccacctga This corresponds to the amino acid sequence <SEQ ID 1158; ORF 281.ng>: g281 -pep 1 MHYALASVFC LSLSAAPVGV FLVMRRMSLI GDALSHAVLP GAAVGYMFAG 51 LSLPAMGVGG FAAGMLMALL AGLVSRFTTL KEDANFAAFY LSS AIGVIL 101 ISKNGSSVDL LHLLFGSVLA VDIPALQLIA AVSGLTLITL AVIYRPLVLE 151 SIDPLFLKSV NGKGGLWHVI FLILWMNLV SGFQALGILM SVGIMMLPAI 201 TARLWARNMG TLILLSVLIA LFCGLIGLLI SYHIEIPSGP AIILCCSVLY 251 LFSVILGKEG GILPKWFKNH RHHTT* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 1159>: m281.seq (partial) 1 ATGCGCTACG CCCTCGCATC CGTCTTCTGC CTGTCCCTCA GTGCCGCACC 51 CGTCGGCGTA TTCCTCGTCA TGCGCCGTAT GAGCCTGATA GGCGACGCAT 101 TGAGCCACGC CGTCCTGCCC GGTGCCGCCG TCGGCTACAT GTTTGCCGGC 151 TTGAGCCTGC CCGCCATGGG TTTGGGCGGC GTAGCCGCAG GCATGCTGAT 201 GGCACTGCTT GCCGGACTCG TCAGCCGCTT CACCACCCTG AAAGAAGATG 251 CCAACTTTGC CGCCTTTTAT CTCAGCAGCC TCGCCATCGG CGTAGTCCTC 301 GTCAGCAAAA ACGGGAGCAG CGTCGATTTG CTCCACCTCC TTTTCGGCTC 351 TGTACTTGCC GTCGATATTC CTGCCCTGCA GCTCATCGCC GCCGTCTCCA 401 GCCTCACGCT CATTACCCTT GCCGTCATCT ACCGCCCGCT CGTACTCGAA 451 AGCATCGACC CCCTGTTTCT CAAATCCGTC GGCGGCAAAG GCGGGCTTTG 501 GCACGTCCTC TTTCTCGTCC TGGTCGTCAT GAACCTCGTA TCCGGCTTTC 551 AAGCCCTCGG CACACTCATG TCCGTCGGAC TCATGATGCT GCCAGCCATT 601 ACCGCCCGCC TGTGGGCGAA GCATATGGGC GCACTCATCC TCCTATCCGT 651 TCTGACAGCC CTGCTGTGCG GCTTGAGCGG ACTGCTCATT TCCTACCACA 701 TCGAAATTCC TTCCGGTCCC GCCATCATCC TCTGTTGCAG CGTCCTTTAT 751 CTCTTTTCCG TCATACTCGG CAAAGAAGGC GGCATTCTGA CC.. This corresponds to the amino acid sequence <SEQ ID 1160; ORF 281>: m281.pep (partial) 1 MRYALASVFC LSLSAAPVGV FLVMRRMSLI GDALSHAVLP GAAVGYMFAG 51 LSLPAMGLGG VAAGMLMALL AGLVSRFTTL KEDANFAAFY LSS AIGWL 101 VSKNGSSVDL LHLLFGSVLA VDIPALQLIA AVSSLTLITL AVIYRPLVLE 151 SIDPLFLKSV GGKGGLWHVL FLVLWMNLV SGFQALGTLM SVGLMMLPAI 201 TARLWAKHMG ALILLSVLTA LLCGLSGLLI SYHIEIPSGP AIILCCSVLY 251 LFSVILGKEG GILT.. Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 281 shows 93.5% identity over a 263 aa overlap with a predicted ORF (ORF 281.ng) from N gonorrhoeae: m281/g281 10 20 30 40 50 60 m281. pep MRYALASVFCLSLSAAPVGVFLVMRRMSLIGDALSHAVLPGAAVGYMFAGLSLPAMGLGG hMMMMMMMMMMMMMMMMMMMMMMMMIMMIhll g281 MHYALASVFCLSLSAAPVGVFLVMRRMSLIGDALSHAVLPGAAVGYMFAGLSLPAMGVGG 10 20 30 40 50 60 70 80 90 100 110 120 m281.pep VAAGMLMALLAGLVSRFTTLKEDAΝFAAFYLSSLAIGWLVSKΝGSSVDLLHLLFGSVLA MMMMMMMMMMMMMMMMMMhhMMMMMMIMMM g281 FAAGMLMALLAGLVSRFTTLKEDAΝFAAFYLSSLAIGVILISKΝGSSVDLLHLLFGSVLA 70 80 90 100 110 120 130 140 150 160 170 180 m281. pep VDIPALQLIAAVSSLTLITLAVIYRPLVLESIDPLFLKSVGGKGGLWHVLFLVLWMΝLV g281 VDIPALQLIAAVSGLTLITLAVIYRPLVLESIDPLFLKSVNGKGGLWHVIFLILWMNLV 130 140 150 160 170 180 190 200 210 220 230 240 m281.pep SGFQALGTLMSVGLMMLPAITARLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGP I M I I I I M M h l M M M I M M = = l h l M M M 1 1 = 1 1 1 l l l l l l l l l l l l l l g281 SGFQALGILMSVGIMMLPAITARLWARNMGTLILLSVLIALFCGLIGLLISYHIEIPSGP 190 200 210 220 230 240 250 260 m281.pep AIILCCSVLYLFSVILGKEGGILT ' MIMMMMMMMMMM g281 AlILCCSVLYLFSVILGKEGGILPKWFKNHRHHTTX 250 260 270 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 1161>: a281.seq 1 ATGCGCTACG CCCTCGCATC CGTCTTCTGC CTGTCCCTCA GTGCCGCACC 51 CGTCGGCGTA TTCCTCGTCA TGCGCCGTAT GAGCCTGATA GGCGACGCAT 101 TGAGCCACGC CGTCCTGCCC GGTGCCGCCG TCGGCTACAT GTTTGCCGGC 151 TTAAGCCTGC CCGCCATGGG TTTGGGCGGC GTAGCCGCAG GTATGCTGAT 201 GGCACTGCTT GCCGGACTCG TCAGCCGCTT CACCACCCTG AAAGAAGATG 251 CCAACTTTGC CGCCTTTTAT CTCAGCAGCC TCGCCATCGG TGTAGTCCTC 301 GTCAGCAAAA ACGGCAGCAG CGTCGATTTG CTCCACCTCC TTTTCGGCTC 351 CGTACTTGCC GTCGATATTC CTGCCCTGCA ACTCATCGCC GCCGTATCCA 401 CCCTCACACT GCTTACCCTT GCCGTCATCT ACCGCCCGCT CGTACTCGAA 451 AGCATCGACC CCCTGTTTCT CAAATCTGTC GGCGGCAAAG GCGGGCTTTG 501 GCACGTCCTC TTTCTCGTCC TGGTCGTCAT GAACCTCGTA TCCGGCTTTC 551 AAGCCCTCGG CACACTCATG TCCGTCGGAC TTATGATGCT GCCAGCCATT 601 ACCGCCCGCC TATGGGCGAA GCACATGGGC GCACTCATCC TCCTATCCGT 651 TCTGACAGCC CTGCTGTGCG GCTTGAGCGG ACTGCTCATT TCCTACCACA 701 TCGAAATTCC TTCCGGTCCC GCCATCATCC TCTGTTGCAG CGTCCTTTAT 751 CTCTTTTCCG TCATACTCGG CAAAGAAGGC GGCATTCTGA CCAAATGGCT 801 CAAAAACCAC CGCCACCACA CCACCTGA This corresponds to the amino acid sequence <SEQ ID 1162; ORF 281.a>: a281.pep 1 MRYALASVFC LSLSAAPVGV FLVMRRMSLI GDALSHAVLP GAAVGYMFAG 51 LSLPAMGLGG VAAGMLMALL AGLVSRFTTL KEDANFAAFY LSSLAIGWL 101 VSKNGSSVDL LHLLFGSVLA VDIPALQLIA AVSTLTLLTL AVIYRPLVLE 151 SIDPLFLKSV GGKGGLWHVL FLVLVVMNLV SGFQALGTLM SVGLMMLPAI 201 TARLWAKHMG ALILLSVLTA LLCGLSGLLI SYHIEIPSGP AIILCCSVLY 251 LFSVILGKEG GILTKWLKNH RHHTT* m28 l/a281 99.2% identity in 264 aa overlap 10 20 30 40 50 60 m281.pep MRYALASVFCLSLSAAPVGVFLVMRRMSLIGDALSHAVLPGAAVGYMFAGLSLPAMGLGG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a281 MRYALASVFCLSLSAAPVGVFLVMRRMSLIGDALSHAVLPGAAVGYMFAGLSLPAMGLGG 10 20 30 40 50 60 70 80 90 100 110 120 m281. pep VAAGMLMALLAGLVSRFTTLKEDANFAAFYLSSLAIGWLVSKNGSSVDLLHLLFGSVLA I I I I I I I I I I I I I II I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a281 VAAGMLMALLAGLVSRFTTLKEDANFAAFYLSSLAIGWLVSKNGSSVDLLHLLFGSVLA 70 80 90 100 110 120 130 140 150 160 170 180 m281.pep VDIPALQLIAAVSSLTLITLAVIYRPLVLESIDPLFLKΞVGGKGGLWHVLFLVLWMNLV I I I I I I I I I I I I I : I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a281 VDIPALQLIAAVSTLTLLTLAVIYRPLVLESIDPLFLKSVGGKGGLWHVLFLVLWMNLV 130 140 150 160 170 180 190 200 210 220 230 240 m281.pep SGFQALGTLMSVGLMMLPAITARLWAKHMGALILLSVLTALLCGLΞGLLISYHIEIPSGP I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a281 SGFQALGTLMSVGLMMLPAITARLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGP 190 200 210 220 230 240 250 260 m281.pep AIILCCSVLYLFSVILGKEGGILT I I I I I I I I I I I I I I I I I II I I I I I a281 AIILCCSVLYLFSVILGKEGGILTKWLKNHRHHTTX 250 260 270 The following partial DNA sequence was identified in N.gonorrhoeae <SEQ ID 1163>: g282.seq 1 atgggattgg gtatggaaat cggcaagctg attgtggctc ttttggtgct 51 gatcaatccg tttagcgcgt tgtcgcttta ccttgacctg accaacggac 101 acagcacgaa ggagcgcagg aaggtcgcgc ggacggccgc cgtcgccgtg 151 tttgccgtga ttgcggtatt tgcgctgatc ggcggtgcgc tattgaaggt 201 tttgggcatc agcgtcggtt cgtttcaggt cggcggcggg attttggtgc 251 tgctgatcgc catttcgatg atgaacggca acgacaatcc cgccaagcag 301 aatctcggcg cgcagccgga aacggggcaa gcgcgccccg cccgcaatgc 351 aggggcgatt gccgtcgtgc ccatcgccat accgatcacc atcggtccgg 401 gcggtatttc gactgtgatt atttatgctt cggcagccaa aacgtacagc 451 gatatcgcgc tgattatcgc ggccggtttg gtggtcagtg cgatttgtta 501 tgccatttta atcgttgccg ggaaggtcag ccgcctgctg ggcgcgacgg 551 ggctgacgat tttaaaccgc attatgggta tgatgctggc ggcggtatcg 601 gtggagatta ttgtgtcggg actgaaaacg atattcccgc aactggcagg 651 ttga This corresponds to the amino acid sequence <SEQ ID 1164; ORF 282.ng>: g282.pep 1 MGLGMEIGKL IVALLVLINP FSALSLYLDL TNGHSTKERR KVARTAAVAV 51 FAVIAVFALI GGALLKVLGI SVGSFQVGGG ILVLLIAISM MNGNDNPAKQ 101 NLGAQPETGQ ARPARNAGAI AWPIAIPIT IGPGGISTVI IYASAAKTYS 151 DIALIIAAGL WSAICYAIL IVAGKVSRLL GATGLTILNR IMGMMLAAVS 201 VEIIVSGLKT IFPQLAG* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 1165>: m282. seq 1 ATGGGATTGG GCATGGAAAT CGGCAAGCTG ATTGTGGCTT TTTTGGTGCT 51 GATTAATCCG TTTAGCGCGT TGTCGCTTTA CCTTGACCTG ACCAACGGGC 101 ACAGCACGAA GGAGCGCAGG AAGGTCGCGC GGACGGCCGC CGTTGCCGTG 151 TTTGCCGTGA TTGCGGTATT TGCGCTGATC GGCGGTACGC TGCTGAAGGT 201 TTTGGGCATC AGCGTCGGTT CGTTTCAGGT CGGCGGCGGG ATTTTGGTGC 251 TGCTGATCGC CATTTCGATG ATGAACGGCA ACGACAATCC CGCCAAGCAG 301 AATCTCGGCG CGCAGCCGGA AACGGGGCAG GCGCGCCCCG CCCGCAATGC 351 CGGAGCGATT GCCGTCGTGC CCATCGCCAT ACCGATCACC ATCGGCCCGG 401 GCGGTATTTC GACCGTGATT ATTTACGCTT CGGCGGCTAA AACATACGGC 451 GACATCGCGT TGATTATCGC GGCCGGTTTG GTGGTCAGTG CGATTTGTTA 501 TGCCATTTTA ATCGTTGCCG GGAAGGTCAG CCGCCTGCTG GGCGCGACGG 551 GGCTGACGAT TTTAAACCGC ATTATGGGTA TGATGCTGGC GGCGGTATCG 601 GTGGAGATTA TTGTGTCGGG ACTGAAAACG ATATTCCCGC AACTGGCAGG 651 TTGA This corresponds to the amino acid sequence <SEQ ID 1166; ORF 282.ng>: m282 -pep 1 MGLGMEIGKL IVAFLVLINP FSALSLYLDL TNGHSTKERR KVARTAAVAV 51 FAVIAVFALI GGTLLKVLGI SVGSFQVGGG ILVLLIAISM MNGNDNPAKQ 101 NLGAQPETGQ ARPARNAGAI AWPIAIPIT IGPGGISTVI IYASAAKTYG 151 DIALIIAAGL WSAICYAIL IVAGKVSRLL GATGLTILNR IMGMMLAAVS 201 VEIIVSGLKT IFPQLAG* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 282 shows 98.6 % identity over a 217 aa overlap with a predicted ORF (ORF 282.ng) from N gonorrhoeae: m282/g282 10 20 30 40 50 60 m282. pep MGLGMEIGKLIVAFLVLIΝPFSALSLYLDLTΝGHSTKERRKVARTAAVAVFAVIAVFALI g282 MGLGMEIGKLIVALLVLIΝPFSALSLYLDLTΝGHSTKERRKVARTAAVAVFAVIAVFALI 10 20 30 40 50 60 70 80 90 100 110 120 m282. pep GGTLLKVLGISVGSFQVGGGILVLLIAlSMMΝGΝDΝPAKQΝLGAQPETGQARPARΝAGAI g282 GGALLKVLGI SVGSFQVGGGI LVLLI Al SMMΝGΝDΝPAKQΝLGAQPETGQARPARΝAGAI 70 80 90 100 110 120 130 140 150 160 170 180 m282 . pep AWPIAIPITIGPGGISTVIIYASAAKTYGDIALI IAAGLWSAICYAILIVAGKVSRLL g282 AWPIAIPITIGPGGISTVIIYASAAKTYSDIALIIAAGLWSAICYAILIVAGKVSRLL 130 140 150 160 170 180 190 200 210 m282 . pep GATGLTILΝRIMGMMLAAVSVEIIVSGLKTIFPQLAGX MMMMMMMMMMMMMMMMMMM g282 GATGLTILΝRIMGMMLAAVSVEIIVSGLKTIFPQLAGX 190 200 210 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1167>: a282.seq 1 ATGGGATTGG GCATGGAAAT CGGCAAGCTG ATTGTGGCTT TTTTGGTGCT 51 GATTAATCCG TTTAGCGCGT TGTCGCTTTA CCTTGACCTG ACCAACGGGC 101 ACAGCACGAA GGAGCGCAGG AAGGTCGCGC GGACGGCCGC CGTTGCCGTG 151 TTTGCCGTGA TTGCGGTATT TGCGCTGATC GGCGGTACGC TGCTGAAGGT 201 TTTGGGCATC AAGGCCGGTTCCGGGGTTTT CGTTTCAGGT CGGCGGCGGA ATTTTGGTGT 251 TGCTGATTGC CATTTCGATG ATGAACGGCA ACGACAATCC CGCCAAGCAG 301 AATCTCGGCG CGCAGCCGGA AACGGGGCAG GTGCGCCCCG CCCGCAATGC 351 CGGAGCGATT GCCGTCGTGC CCATCGCCAT ACCGATCACC ATCGGCCCGG 401 GCGGTATTTC GACCGTGATT ATTTACGCTT CGGCGGCTAA AACATACGGC 451 GACATCGCGT TGATTATCGC GGCCGGTTTG GTGGTCAGTG CGATTTGTTA 501 TGCCATTTTA ATCGTTGCCG GGAAGGTCAG CCGCCTGCTG GGTGCGACGG 551 GGCTGACGAT TTTAAACCGT ATCATGGGTA TGATGCTGGC GGCGGTATCG 601 GTGGAGATTA TTGTGTCGGG ACTGAAAATG ATATTCCCGC AACTGGCAGG 651 TTGA This corresponds to the amino acid sequence <SEQ ID 1168; ORF 282. a>: a282.pep 1 MGLGMEIGKL IVAFLVLINP FSALSLYLDL TNGHSTKERR KVARTAAVAV 51 FAVIAVFALI GGTLLKVLGI SVGSFQVGGG ILVLLIAISM MNGNDNPAKQ 101 NLGAQPETGQ VRPARNAGAI AWPIAIPIT IGPGGISTVI IYASAAKTYG 151 DIALIIAAGL WSAICYAIL IVAGKVSRLL GATGLTILNR IMGMMLAAVS 201 VEIIVSGLKM IFPQLAG^ m282/a282 99.1 % identity in 217 aa overlap 10 20 30 40 50 60 m282.pep MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHΞTKERRKVARTAAVAVFAVIAVFALI I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a282 MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVARTAAVAVFAVIAVFALI 10 20 30 40 50 60 70 80 90 100 110 120 m282.pep GGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNLGAQPETGQARPARNAGAI I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I :| I I I I I I I I a282 GGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNLGAQPETGQVRPARNAGAI 70 80 90 100 110 120 130 140 150 160 170 180 m282.pep AVVPIAIPITIGPGGIΞTVIIYASAAKTYGDIALIIAAGLVVSAICYAILIVAGKVSRLL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a282 AVVPIAIPITIGPGGISTVIIYAΞAAKTYGDIALIIAAGLVVSAICYAILIVAGKVSRLL 130 140 150 160 170 180 190 200 210 πι282.pep GATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAGX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I llllllll a282 GATGLTILNRIMGMMLAAVSVEIIVSGLKMIFPQLAGX 190 200 210 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1169>: g283. seq 1 atgaactttg ctttatccgt catcacattt accctcgcct ctttcctgcc 51 cgtcccgcct gccggaaccg ccgtctttac ttggaaagac ggcggcggca 101 acagctattc ggatgtgccg aaacagcttc atcccgacca gagccaaatc 151 ctcaacctgc ggacgctcca aaccaaaccg gcggtcaagc ccaaacctgc 201 cgtcgatacg aatgcggaca gtgcgaagga aaacgaaaag gatatcgccg 251 agaaaaacgg gcagcttgag gaagaaaaga aaaaaattgc cgaaaccgaa 301 cggcagaaca aagaagaaaa ctgccggatt tcaaaaatga acctgaaggc 351 ggtgggaaac tcaaatgcga aaaacaagga tgatttgatc cgtaaataca 401 ataacgccgt aaacaaatac tgccgttaa This corresponds to the amino acid sequence <SEQ ID 1170; ORF 283. ng>: g283.pep 1 MΝFALSVITF TLASFLPVPP AGTAVFTWKD GGGΝSYSDVP KQLHPDQSQI 51 LΝLRTLQTKP AVKPKPAVDT ΝADSAKEΝEK DIAEKΝGQLE EEKKKIAETE 101 RQΝKEEΝCRI SKMΝLKAVGΝ SΝAKΝKDDLI RKYΝΝAVΝKY CR* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1171>: m283. seq 1 ATGAACTTTG CTTTATCCGT CATTATGTTG ACCCTCGCCT CTTTCCTGCC 51 CGTCCCGCCT GCCGGAGCCG CCGTCTTTAC TTGGAAGGAC GGCGGCGGCA 101 ACAGCTATTC GGATGTACCG AAACAGCTTC ATCCCGACCA AAGCCAAATC 151 TTAAACCTGC GGACGCGCCA AACCAAACCG GCGGTCAAAC CCGCCCAAGC 201 CGACGCAGGG AAGCGCACAG ACGGCGCGGC ACAGGAAAAC AATCCCGACA 251 CTGCCGAGAA AAACCGGCAG CTTGAGGAAG AAAAGAAAAG AATTGCCGAA 301 ACCGAACGGC AGAACAAAGA AGAAAACTGC CGGATTTCAA AAATGAACCT 351 GAAGGCGGTG GGAAATTCAA ATGCAAAAAA CAAGGATGAT TTGATTCGGA 401 AATACAATAA CGCCGTAAAC AAATACTGCC GTTAA This corresponds to the amino acid sequence <SEQ ID 1172; ORF 283>: m283.pep 1 MΝFALSVIML TLASFLPVPP AGAAVFTWKD GGGΝSYSDVP KQLHPDQSQI 51 LΝLRTRQTKP AVKPAQADAG KRTDGAAQEΝ ΝPDTAEKΝRQ LEEEKKRIAE 101 TERQΝKEEΝC RISKMΝLKAV GΝSΝAKΝKDD LIRKYΝΝAVΝ KYCR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m283/g283 86.1% identity in 144 aa overlap 10 20 30 40 50 60 m283.pep MΝFALSVIMLTLASFLPVPPAGAAVFTWKDGGGΝSYSDVPKQLHPDQSQILΝLRTRQTKP llllllll :| I I I I I I I I I I 1:1 I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I llll g283 MΝFALSVITFTLASFLPVPPAGTAVFTWKDGGGΝSYSDVPKQLHPDQSQILΝLRTLQTKP 10 20 30 40 50 60 70 80 90 100 110 120 m283.pep AVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETERQNKEENCRISKMNLKAV llll I : : I : I : I I : I llll I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I g283 AVKPKPA-VDTNAD-SAKENEKDIAEKNGQLEEEKKKIAETERQNKEENCRISKMNLKAV 70 80 90 100 110 130 140 m283. pep GNSNAKNKDDLIRKYNNAVNKYCRX I I I I I I I I I I I I I I I I I I I I I I I I I g283 GNSNAKNKDDLIRKYNNAVNKYCRX 120 130 140 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1173>: a283. seq 1 ATGAACTTTG CTTTATCCGT CATTATGTTG ACCCTCGCCT CTTTCCTGCC 51 CGTCCCGCCT GCCGGAGCCG CCGTCTTTAC TTGGAAGGAC GGCGGCGGCA 101 ACAGCTATTC GGATGTACCG AAACAGCTTC ATCCCGACCA AAGCCAAATC 151 TTAAACCTGC GGACGCGCCA AACCAAACCG GCGGTCAAAC CCGCCCAAGC 201 CGACGCAGGG AAGCGCACAG ACGGCGCGGC ACAGGAAAAC AATCCCGACA 251 CTGCCGAGAA AAACCGGCAG CTTGAGGAAG AAAAGAAAAG AATTGCCGAA 301 ACCGAACGGC AGAACAAAGA AGAAAACTGC CGGATTTCAA AAATGAACCT 351 GAAAGCGGTG GGAAATTCAA ATGCAAAAAA CAAGGATGAT TTGATTCGGA 401 AATACAATAA CGCCGTAAAC AAATACTGCC GTTAA This corresponds to the amino acid sequence <SEQ ID 1174; ORF 283. a>: a283.pep 1 MΝFALSVIML TLASFLPVPP AGAAVFTWKD GGGΝSYSDVP KQLHPDQSQI 51 LΝLRTRQTKP AVKPAQADAG KRTDGAAQEΝ ΝPDTAEKΝRQ LEEEKKRIAE 101 TERQΝKEEΝC RISKMΝLKAV GΝSΝAKΝKDD LIRKYΝΝAVΝ KYCR* m283/a283 100.0% identity in 144 aa overlap 10 20 30 40 50 60 m283.pep MΝFALSVIMLTLASFLPVPPAGAAVFTWKDGGGΝSYSDVPKQLHPDQSQILΝLRTRQTKP I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I a283 MΝFALSVIMLTLASFLPVPPAGAAVFTWKDGGGΝSYSDVPKQLHPDQSQILΝLRTRQTKP 10 20 30 40 50 60 70 80 90 100 110 120 m283.pep AVKPAQADAGKRTDGAAQEΝΝPDTAEKΝRQLEEEKKRIAETERQΝKEEΝCRISKMΝLKAV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a283 AVKPAQADAGKRTDGAAQEΝΝPDTAEKΝRQLEEEKKRIAETERQΝKEEΝCRISKMΝLKAV 70 80 90 100 110 120 130 140 m283. pep GNSNAKNKDDLIRKYNNAVNKYCRX I I I I I I I I I I I I I I I I I I I I I I I I I a283 GNSNAKNKDDLIRKYNNAVNKYCRX 130 140 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1175>: g284. seq. 1 atgccgtctg aaactcgaaa tcggtttcag acggcattgg tttacgcggc 51 aggttggggc ttagcggtct ttgtaacggc attcgctttt gcctgcaaaa 101 gagtcgccgg ctttgcgttt gcctttgaag ccttcgccgg tttttttgaa 151 actgtctttc ttaaagcctt ctttcttgaa accttcgccg cgcgttttgc 201 cgccgaagcc ttctttgccc ggtttatgat cgccgcgccg gccgccggat 251 ttcctatcgc cccagccgcc tttgcctttc ggcttgccgc ctgcggattt 301 gcgtttgcgg gccggctcca tgccttcgat ggtcagttcg ggcagtttgc 351 ggttaatgta tttttcgatt ttgtggactt tgacgtattc gttcacttcg 401 gcaaacgtaa tcgcaatacc cgtgcggcct gcgcggccgg tgcgcccgat 451 gcggtggacg tagtcttccg cctgtttcgg caggtcgtag tttatgacgt 501 gggtaatggt cggtacgtca ataccgcgtg cggcaacgtc ggtggcaacc 551 aaaattttgc agcggccttt acgcaaatcc gtcagcgtgc ggttgcgcca 601 gccctgcggc atatcgccgt gcaggcagtt ggcggcgaaa cctttttcgt 651 acaattcatc cgcgatgact tcggtcatcg ctttggtgga cgtgaaaatc 701 acacattggt cgatgttggc atcgcgcagg atgtggtcga gcaggcggtt 751 tttgtggcgc atatcgtcgc agtacaacaa ctgctcttcg attttgcctt 801 ggccgtccac gcgttcgact tcgataattt cagagtcttt ggtcagtttg 851 cgcgccagtt tgccgactgc gccgtcccaa gtggcggaga acaataa This corresponds to the amino acid sequence <SEQ ID 1176; ORF 284.ng>: g284.pep 1 MPSETRNRFQ TALVYAAGWG LAVFVTAFAF ACKRVAGFAF AFEAFAGFFE 51 TVFLKAFFLE TFAARFAAEA FFARFMIAAP AAGFPIAPAA FAFRLAACGF 101 AFAGRLHAFD GQFGQFAVNV FFDFVDFDVF VHFGKRNRNT RAACAAGAPD 151 AVDWFRLFR QVWYDVGNG RYVNTACGNV GGNQNFAAAF TQIRQRAVAP 201 ALRHIAVQAV GGETFFVQFI RDDFGHRFGG RENHTLVDVG IAQDWEQAV 251 FVAHIVAVQQ LLFDFALAVH AFDFDNFRVF GQFARQFADC AVPSGGEQ* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1177>: m284.seq.. 1 ATGCCGTCTG AAACTCGAAA TCGGTTTCAG ACGGCATTGG TTTATGCGGC 51 AGGTTGGGGC TTAGCGGTCT TTGTAACGGC GTTCGCCTTT GCCTGCAAAA 101 GAATCGCCGG CTTTGCGTTT GCCTTTGAAG CCTTCGCCGG TTTTTTTGAA 151 ACCGTCTCTC TTAAAGCCTT CTTTCTTGAA ACCTTCGCCG CGCGTTTTGC 201 CGCCGAAGCC TTCTTTGCTC GGTTTATGAT CGCCGCGCCA ACCGCCGGAT 251 TTACGATCGC CCCAGCCGCC TTTGCCTTTC GGCTTGCCGC CTGCGGATTT 301 GCGTTTGCGG GTCGGTTCCA TGCCTTCGAT GGTCAGTTCG GGCAGTTTTC 351 GGTTAATGTA TTTTTCGATT TTGTGGACTT TGACGTATTC GTTCACTTCG 401 GCAAACGTAA TCGCAATACC CGTGCGGCCT GCGCGGCCGG TGCGCCCGAT 451 GCGGTGGACG TAGTCTTCCG CCTGTTTCGG CAGGTCGTAG TTGATAACGT 501 GGGTAATGGT CGGTACGTCG ATACCGCGTG CGGCAACATC GGTGGCAACC 551 AAAATTTTGC AGCGGCCTTT ACGCAAATCC ATCAGCGTGC GGTTGCGCCA 601 GCCTTGCGGC ATATCGCCGT GCAGGCAGTT TGCGGCGAAA CCTTTTTCGT 651 ACAGTTCATC CGCAATGACT TCGGTCATGG CTTTGGTGGA CGTGAAAATC 701 ACGCATTGAT CGATATTGGC ATCGCGCAAG ATATGATCGA GCAGGCGGTT 751 TTTGTGGCGC ATATCGTCGC AGTACAGCAG TTGTTCTTCG ATTTTGCCTT 801 GATCGTCCAC GCGTTCGACT TCGATGATTT CAGGGTCTTT GGTCAGTTTG 851 CGCGCCAGTT TGCCGACCGC GCCGTCCCAA GTGGCGGAGA ACAACAAAGT 901 CTGACGGTCG CTCGGCGTTG CTTCCACGAT GGTTTCGATG TCGTCGATAA 951 AGCCCATATC CAACATACGG TCGGCTTCGT CCAAAATCAG CACTTCCAAA 1001 CGTTCAAAAT CAACTTTGCC GCTTTGCATC AGGTCCATCA GACGGCCCGG 1051 CGTGGCGACA ATCAGATCGA CCGGTTTGCT CAGGGCACGG GTTTGGTAGC 1101 CGAAAGACGC GCCGCCGACG ATGCTGACGG TGCGGAACCA ACGCATATTT 1151 TTGGCATACG CCAGCGCGTT TTTCTCGACT TGAGCCGCCA GTTCGCGGGT 1201 CGGGGTCAAC ACCAAAGCAC GCGGGCCTTT GCCCGGTTTT TCGCTGCGTT 1251 TGGTCAGTTT TTGCAAAGTC GGTAA This corresponds to the amino acid sequence <SEQ ID 1178; ORF 284>: m284.pep 1 MPSETRNRFQ TALVYAAGWG LAVFVTAFAF ACKRIAGFAF AFEAFAGFFE 51 TVSLKAFFLE TFAARFAAEA FFARFMIAAP TAGFTIAPAA FAFRLAACGF 101 AFAGRFHAFD GQFGQFSVNV FFDFVDFDVF VHFGKRNRNT RAACAAGAPD 151 AVDWFRLFR QVWDNVGNG RYVDTACGNI GGNQNFAAAF TQIHQRAVAP 201 ALRHIAVQAV CGETFFVQFI RNDFGHGFGG RENHALIDIG IAQDMIEQAV 251 FVAHIVAVQQ LFFDFALIVH AFDFDDFRVF GQFARQFADR AVPSGGEQQS 301 LTVARRCFHD GFDWDKAHI QHTVGFVQNQ HFQTFKINFA ALHQVHQTAR 351 RGDNQIDRFA QGTGLVAERR AADDADGAEP THIFGIRQRV FLDLSRQFAG 401 RGQHQSTRAF ARFFAAFGQF LQSR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae πι284/g284 92 . 3% identity in 298 aa overlap 10 20 30 40 50 60 m284. pep MPSETRNRFQTALVYAAGWGLAVFVTAFAFACKRIAGFAFAFEAFAGFFETVSLKAFFLE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I lllllll g284 MPSETRNRFQTALVYAAGWGLAVFVTAFAFACKRVAGFAFAFEAFAGFFETVFLKAFFLE 10 20 30 40 50 60 70 80 90 100 110 120 m284. pep TFAARFAAEAFFARFMIAAPTAGFTIAPAAFAFRLAACGFAFAGRFHAFDGQFGQFSVNV I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I : I I I g284 TFAARFAAEAFFARFMIAAPAAGFPIAPAAFAFRLAACGFAFAGRLHAFDGQFGQFAVNV 70 80 90 100 110 120 130 140 150 160 170 180 m284 . pep FFDFVDFDVFVHFGKRNRNTRAACAAGAPDAVDWFRLFRQWVDNVGNGRYVDTACGNI I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I h I I I I I : g284 FFDFVDFDVFVHFGKRNRNTRAACAAGAPDAVDWFRLFRQWVYDVGNGRYVNTACGNV 130 140 150 160 170 180 190 200 210 220 230 240 m284 . pep GGNQNFAAAFTQIHQRAVAPALRHIAVQAVCGETFFVQFIRNDFGHGFGGRENHALIDIG I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I : I : I : I g284 GGNQNFAAAFTQIRQRAVAPALRHIAVQAVGGETFFVQFIRDDFGHRFGGRENHTLVDVG 190 200 210 220 230 240 250 260 270 280 290 300 m284 . pep IAQDMIEQAVFVAHIVAVQQLFFDFALIVHAFDFDDFRVFGQFARQFADRAVPSGGEQQS I I I I : : I I I I I I I I I I I I I I I : I I I I I I I I I I I I : I I I I I I I I I I I I I l l l l l l l l g284 IAQDWEQAVFVAHIVAVQQLLFDFALAVHAFDFDNFRVFGQFARQFADCAVPSGGEQX 250 260 270 280 290 310 320 330 340 350 360 m284.pep LTVARRCFHDGFDWDKAHIQHTVGFVQNQHFQTFKINFAALHQVHQTARRGDNQIDRFA The following partial DNA sequence was identified in N meningitidis <SEQ ID 1179>: a284.seq 1 ATGCCGTCTG AAACTCGAAA TCGGTTTCAG ACGGCATTGG TTTATGCGGC 51 AGGTTGGGGC TTAGCGGTCT TTGTAACGGC GTTCGCCTTT GCCTGCAAAA 101 GAATCGCCGG CTTTGCGTTT GCCTTTGAAG CCTTCGCCGG TTTTTTTGAA 151 ACCGTCTCTC TTAAAGCCTT CTTTCTTGAA ACCTTCGCCG CGCGTTTTGC 201 CGCCGAAGCC TTCTTTGCTC GGTTTATGAT CGCCGCGCCA ACCGCCGGAT 251 TTACGATCGC CCCAGCCGCC TTTGCCTTTC GGCTTGCCGC CTGCGGATTT 301 GCGTTTGCGG GTCGGTTCCA TGCCTTCGAT GGTCAGTTCG GGCAGTTTTC 351 GGTTAATGTA TTTTTCGATT TTGTGGACTT TGACGTATTC GTTCACTTCG 401 GCAAACGTAA TCGCAATACC CGTGCGGCCT GCGCGGCCGG TGCGCCCGAT 451 GCGGTGGACG TAGTCTTCCG CCTGTTTCGG CAGGTCGTAG TTGATAACGT 501 GGGTAATGGT CGGTACGTCG ATACCGCGTG CGGCAACGTC GGTGGCAACC 551 AAAATTTTGC AGCGGCCTTT GCGCAAATCC ATCAGCGTGC GGTTGCGCCA 601 GCCTTGCGGC ATATCGCCGT GCAGGCAGTT GGCGGCGAAA CCTTTTTCGT 651 ACAATTCATC CGCGATGACT TCGGTCATGG CTTTGGTGGA CGTGAAAATC 701 ACGCATTGAT CGATGTCGGC ATCGCGCAAG ATATGATCGA GCAGGCGGTT 751 TTTGTGGCGC ATATCGTCGC AGTACAGCAG TTGTTCTTCG ATTTTGCCTT 801 GGTCGTCCAC GCGTTCGACT TCGATGATTT CAGGGTCTTT GGTCAGTTTG 851 CGCGCCAGTT TGCCGACCGC GCCGTCCCAA GTGGCGGAGA ACAACAAAGT 901 CTGACGGTCT TCCGGCGTGG CTTCGACGAT GGTTTCGATG TCGTCGATAA 951 AGCCCATATC CAACATACGG TCGGCTTCGT CCAAAATCAG CACTTCCAAG 1001 CGGGCGAAAT CGACTTTGCC GCTTTGCATC AAGTCCATCA GACGGCCCGG 1051 CGTGGCGACA ATCAGATCGA CCGGTTTGCT CAGGGCGCGG GTTTGGTAGC 1101 CGAACGATGC ACCACCGACG ATGCTGACGG TACGGAACCA ACGCATATTT 1151 TTGGCATACG CCAGCGCGTT TTTCTCGACT TGAGCCGCCA ATTCGCGGGT 1201 CGGCGTCAAC ACCAACGCGC GCGGGCCTTT GCCCGGTTTT TCGCTGCGTT 1251 TGGTCAGTCG CTGCAAAGTC GGTAA This corresponds to the amino acid sequence <SEQ ID 1180; ORF 284.a>: a284.pep 1 MPSETRNRFQ TALVYAAGWG LAVFVTAFAF ACKRIAGFAF AFEAFAGFFE 51 TVSLKAFFLE TFAARFAAEA FFARFMIAAP TAGFTIAPAA FAFRLAACGF 101 AFAGRFHAFD GQFGQFSVNV FFDFVDFDVF VHFGKRNRNT RAACAAGAPD 151 AVDWFRLFR QVWDNVGNG RYVDTACGNV GGNQNFAAAF AQIHQRAVAP 201 ALRHIAVQAV GGETFFVQFI RDDFGHGFGG RENHALIDVG IAQDMIEQAV 251 FVAHIVAVQQ LFFDFALWH AFDFDDFRVF GQFARQFADR AVPSGGEQQS 301 LTVFRRGFDD GFDWDKAHI QHTVGFVQNQ HFQAGEIDFA ALHQVHQTAR 351 RGDNQIDRFA QGAGLVAERC TTDDADGTEP THIFGIRQRV FLDLSRQFAG 401 RRQHQRARAF ARFFAAFGQS LQSR* m284/a284 94.8% identity in 424 aa overlap 10 20 30 40 50 60 m284.pep MPSETRNRFQTALVYAAGWGLAVFVTAFAFACKRIAGFAFAFEAFAGFFETVSLKAFFLE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I II I I I I I I I I I I I I I I I II I I I a284 MPSETRNRFQTALVYAAGWGLAVFVTAFAFACKRIAGFAFAFEAFAGFFETVSLKAFFLE 10 20 30 40 50 60 70 80 90 100 110 120 m284. pep TFAARFAAEAFFARFMIAAPTAGFTIAPAAFAFRLAACGFAFAGRFHAFDGQFGQFSVNV I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a284 TFAARFAAEAFFARFMIAAPTAGFTIAPAAFAFRLAACGFAFAGRFHAFDGQFGQFSVNV 70 80 90 100 110 120 130 140 150 160 170 180 m284. pep FFDFVDFDVFVHFGKRNRNTRAACAAGAPDAVDWFRLFRQVWDNVGNGRYVDTACGNI I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : a284 FFDFVDFDVFVHFGKRNRNTRAACAAGAPDAVDWFRLFRQVWDNVGNGRYVDTACGNV 130 140 150 160 170 180 190 200 210 220 230 240 m28 . pep GGNQNFAAAFTQIHQRAVAPALRHIAVQAVCGETFFVQFIRNDFGHGFGGRENHALIDIG I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I : I a28 GGNQNFAAAFAQIHQRAVAPALRHIAVQAVGGETFFVQFIRDDFGHGFGGRENHALIDVG 190 200 210 220 230 240 250 260 270 280 290 300 m28 . pep IAQDMIEQAVFVAHIVAVQQLFFDFALIVHAFDFDDFRVFGQFARQFADRAVPSGGEQQS I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a284 IAQDMIEQAVFVAHIVAVQQLFFDFALWHAFDFDDFRVFGQFARQFADRAVPSGGEQQS 250 260 270 280 290 300 310 320 330 340 350 360 m284. pep LTVARRCFHDGFDWDKAHIQHTVGFVQNQHFQTFKINFAALHQVHQTARRGDNQIDRFA III II I I I I I I I I I I I I I I I I I I I I I I I I I : : I : I I I I I I I I I I I I I I I I I I I I I I a284 LTVFRRGFDDGFDWDKAHIQHTVGFVQNQHFQAGEIDFAALHQVHQTARRGDNQIDRFA 310 320 330 340 350 360 370 380 390 400 410 420 m28 . pep QGTGLVAERRAADDADGAEPTHIFGIRQRVFLDLSRQFAGRGQHQSTRAFARFFAAFGQF I I : I I I I I I : : I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I a284 QGAGLVAERCTTDDADGTEPTHIFGIRQRVFLDLSRQFAGRRQHQRARAFARFFAAFGQS 370 380 390 400 410 420 m284 . pep LQSRX M i l l a284 LQSRX The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1181>: g285. seq 1 atgaccgata ccacaccgac agataccgat ccgaccgaaa acggcacgcg 51 caaaatgccg tctgaacacc gccccgcccc gccggcaaaa aaacgccgcc 101 cgctgctgaa gctgtcggcg gcactgctgt ctgtcctgat tttggcagta 151 tgtttcctcg gctggatcgc cggtacggaa gcaggtttgc gcttcgggct 201 gtaccaaatc ccgtcctggt tcggcgtaaa catttcctcc caaaacctca 251 aaggcacact gctcgacggc ttcgacggcg acaactggtc gatagaaacc 301 gagggggcag accttaaaat cagccgcttc cgcttcgcgt ggaaaccgtc 351 cgaactgatg cgccgcagcc tgcacatcac cgacatctcc gccggcgaca 401 tcgccatcgt aaccaaaccg actccgccta aagaagaacg cccgcctcaa 451 ggcctgcccg acagcataga cctgcccgcc gctgtctatc tcgaccgctt 501 cgagacgggc aaaatcagca tgggcaaaac ctttgacaaa caaaccgtct 551 atctcgaacg cctcaacgcg gcataccgtt acgaccgtaa agggcaccgc 601 ctcgacctga aggccgccga cacgccgtgg agcagttcgt cggggtcagc 651 ctcggtcggc ttgaaaaaac cgtttgccct cgataccgcc atttacacca 701 aaggcggatt cgaaggcgaa accatacaca gtacggcgcg gctgagcggc 751 agcctgaagg atgtgcgcgc cgaactgacg atcgacggcg gcaatatccg 801 cctctcggga aaatccgtca tccacccgtt tgccgaatca ttggataaaa 851 cattggaaga agtactggtc aaaggattca acatcaatcc gtccgccttc 901 gtgccttccc tgcccgatgc cgggctgaat ttcgacctga ccgccatccc 951 gtcgttttca gacggcatcg cgctggaagg ctcgctcgat ttggaaaaca 1001 ccaaagccgg ctttgccgac cgcaacggca tccccgtccg tcaggttttg 1051 ggcggctttg tcatccggca ggacggcacg gtgcatatcg gcaatacgtc 1101 cgccgccctg ctcggacggg gcggcatcag gctgtcgggc aaaatcgaca 1151 ccgaaaaaga catccttgat ttaaatatag gcatcaactc cgtcggcgcg 1201 gaagacgtgc tgcaaaccgc gttcaaaggc aggttggacg gcagcatcgg 1251 catcggcggc acgaccgcct cgcccaaaat ctcttggcaa ctcggcaccg 1301 gcacggcacg cacggacggc agcctcccca tcgcaagcga ccccgcaaac 1351 gaacagcgga aactggtgtt cgacaccgtc aacatctccg ccggggaagg 1401 cagcctgacc gcgcaaggct atctcgagct gtttaaagac cgcctgctca 1451 agctggacat ccgttcccgc gcattcgacc cttcgcgcat cgatccgcaa 1501 tttccggcag gcaatatcaa cggttcgatt catcttgccg gtgaactggc 1551 aaaagagaaa tttacgggca aaatgcgttt tttgcccggt acgttcaacg 1601 gcgtgccgat tgccggcagc gccgacattg tttacgagtc ccgccacctt 1651 ccgcgcgccg ccgtcgattt gcggttgggg cggaacatcg tcaaaacaga 1701 cggcggcttc ggcaaaaaag gcgaccggct taacctcaat atcaccgcac 1751 ccgatttatc ccgtttcggt ttcggactcg cggggtcttt aaatgtacgc 1801 ggacaccttt ccggcgattt ggacggcggc atccgaacct ttgaaaccga 1851 cctttccggc acggcgcgca acttacacat cggcaaagcg gcagacatcc 1901 gttcgctcga ttttaccctc aaaggctcac ccggcacaag ccgcccgatg 1951 cgcgccgata tcaagggcgg ccgcctttcc ctgtcgggcg gcgcggcggt 2001 tgtcgatacc gccggcctga cgctggaagg tacgggcgcg cagcaccgca 2051 tccgcacaca cgccgccatg acgctggacg gcaaaccgtt caaactcgat 2101 ttggacgctt caggcggcat caacagggaa cttacccgat ggaaaggcag 2151 catcggcatc ctcgacatcg gcggcgcatt caacctcaag ctgcaaaacc 2201 gtatgacgct cgaagccggt gcggaacacg tggcggcaag tgcggcaaat 2251 tggcaggcaa tgggcggcag cctcaacctg caacactttt cttgggacag 2301 gaaaaccggc atatcggcaa aaggcggcgc acgcggcctg cacatcgccg 2351 agttgcacaa tttcttcaaa ccgcccttcg aacacaatct ggttttaaac 2401 ggcgactggg atgtcgccta cgggcacaac gcgcgcggct acctcaatat 2451 cagccggcaa agcggcgatg ccgtattgcc cggcgggcag gctttgggtt 2501 tgaacgcatt ttccctgaaa acgcgctttc aaaacgaccg catcggaatc 2551 ctgcttgacg gcggcgcgcg tttcggacgg attaacgccg atttgggcat 2601 cggcaacgcc ttcggcggca atatggcaaa tacaccgctc ggcggcagga 2651 ttacagcctc ccttcccgac ttgggcgcat tgaagccctt tctgcccgcc 2701 gccgcgcaaa acattaccgg cagcctgaat gcctccgcgc aaatcggcgg 2751 acgggtaggc tctccgtccg tcaatgccgc cgtcaacggt agcagcaact 2801 acgggaaaat caacggcaat atcaccgtcg ggcaaagccg ctccttcgat 2851 accgcacctt tgggcggcag gctcaacctg accgttgccg atgccgaagc 2901 attccgcaac ttcctaccgg tcggacaaac cgtcaaaggc agcctgaatg 2951 ccgccgtaac cctcggcggc agcatcgccg acccgcactt gggcggcagt 3001 atcaacggcg acaagctcta ttaccgcaac caaacccaag gcatcatctt 3051 ggacaacggc tcgctgcgtt cgcatattgc aggcaggaaa tgggtaatcg 3101 acagcctgaa attccggcac gaagggacgg cggaactctc cggcacggtc 3151 agcatggaaa acagcgtgcc cgatgtcgat atcggcgcgg tgttcgacaa 3201 ataccgcatc ctgtcccgcc ccaaccgccg cctgacggtt tccggcaaca 3251 cccgcctgcg ctattcgccg caaaaaggca tatccgttac cggtatgatt 3301 aaaactgatc aggggctgtt cggttcgcaa aaatcctcga tgccgtccgt 3351 cggcgacgat gtcgtcgtat tgggcgaagt caagaaagag gcggcggcat 3401 cgctccccgt caatatgaac ctgactttag acctcaatga cggcatccgc 3451 ttctccggct acggcgcgga cgttaccata ggcggcaaac tgaccctgac 3501 cgcgcaaccg ggcggaaatg tgcgtggggt gggcacggtc cgcgtcatca 3551 aagggcgtta caaagcatac gggcaggatt tagacattac caaaggcaca 3601 gtctcctttg tcggcccgct caacgacccc aacctgaaca tccgcgccga 3651 acgccgcctt tcccccgtcg gtgcgggcgt ggaaatattg ggcagcctca 3701 acagcccgcg cattacgctg acggcaaacg aaccgatgag tgaaaaagac 3751 aagctctcct ggctcatcct caaccgtgcc ggcagcggca gcagcggcga 3801 caatgccgcc ctgtccgcag ccgcaggcgc gctgcttgcc gggcaaatca 3851 acgaccgcat cgggctggtg gatgatttgg gctttaccag caagcgcagc 3901 cgcaacgcgc aaaccggcga actcaacccc gccgaacagg tgctgaccgt 3951 cggcaaacaa ctgaccggca aactctacat cggctacgaa tacggcatct 4001 ccagcgcgga acagtccgtc aaactgattt accggctgac ccgcgccata 4051 caggcggttg cccgtatcgg cagccgttcg tcgggcggcg agctgacata 4101 caccatacgt ttcgaccgcc tcttcggttc ggacaaaaaa gactccgcag 4151 gaaacggcaa agggaaataa This corresponds to the amino acid sequence <SEQ ID 1182; ORF 285. ng>: g285.pep 1 MTDTTPTDTD PTENGTRKMP SEHRPAPPAK KRRPLLKLSA ALLSVLILAV 51 CFLGWIAGTE AGLRFGLYQI PSWFGVNISS QNLKGTLLDG FDGDNWSIET 101 EGADLKISRF RFAWKPSELM RRSLHITDIS AGDIAIVTKP TPPKEERPPQ 151 GLPDSIDLPA AVYLDRFETG KISMGKTFDK QTVYLERLNA AYRYDRKGHR 201 LDLKAADTPW SSSSGSASVG LKKPFALDTA IYTKGGFEGE TIHSTARLSG 251 SLKDVRAELT IDGGNIRLSG KSVIHPFAES LDKTLEEVLV KGFNINPSAF 301 VPSLPDAGLN FDLTAIPSFS DGIALEGSLD LENTKAGFAD RNGIPVRQVL 351 GGFVIRQDGT VHIGNTSAAL LGRGGIRLSG KIDTEKDILD LNIGINSVGA 401 EDVLQTAFKG RLDGSIGIGG TTASPKISWQ LGTGTARTDG SLPIASDPAN 451 EQRKLVFDTV NISAGEGSLT AQGYLELFKD RLLKLDIRSR AFDPSRIDPQ 501 FPAGNINGSI HLAGELAKEK FTGKMRFLPG TFNGVPIAGS ADIVYESRHL 551 PRAAVDLRLG RNIVKTDGGF GKKGDRLNLN ITAPDLSRFG FGLAGSLNVR 601 GHLSGDLDGG IRTFETDLSG TARNLHIGKA ADIRSLDFTL KGSPGTSRPM 651 RADIKGGRLS LSGGAAWDT AGLTLEGTGA QHRIRTHAAM TLDGKPFKLD 701 LDASGGINRE LTRWKGSIGI LDIGGAFNLK LQNRMTLEAG AEHVAASAAN 751 WQAMGGSLNL QHFSWDRKTG ISAKGGARGL HIAELHNFFK PPFEHNLVLN 801 GDWDVAYGHN ARGYL ISRQ SGDAVLPGGQ ALGLNAFSLK TRFQNDRIGI 851 LLDGGARFGR INADLGIGNA FGGNMANTPL GGRITASLPD LGALKPFLPA 901 AAQNITGSLN ASAQIGGRVG SPSVNAAVNG SSNYGKINGN ITVGQSRSFD 951 TAPLGGRLNL TVADAEAFRN FLPVGQTVKG SLNAAVTLGG SIADPHLGGS 1001 INGDKLYYRN QTQGIILDNG SLRSHIAGRK WVIDSLKFRH EGTAELSGTV 1051 SMENSVPDVD IGAVFDKYRI LSRPNRRLTV SGNTRLRYSP QKGISVTGMI 1101 KTDQGLFGSQ KSSMPSVGDD VWLGEVKKE AAASLPVNMN LTLDLNDGIR 1151 FSGYGADVTI GGKLTLTAQP GGNVRGVGTV RVIKGRYKAY GQDLDITKGT 1201 VSFVGPLNDP NLNIRAERRL SPVGAGVEIL GSLNSPRITL TANEPMSEKD 1251 KLSWLILNRA GSGSSGDNAA LSAAAGALLA GQINDRIGLV DDLGFTSKRS 1301 RNAQTGELNP AEQVLTVGKQ LTGKLYIGYE YGISSAEQSV KLIYRLTRAI 1351 QAVARIGSRS SGGELTYTIR FDRLFGSDKK DSAGNGKGK* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1183>: m285. seq 1 ATGACCGATA CCGCACCGAC AGATACCGAT CCGACCGAAA ACGGCACGCG 51 CAAAATGCCG TCTGAACACC GCCCTACCCC GCCGGCAAAA AAACGCCGCC 101 CGTTGCTGAA GCTGTCGGCG GCACTGCTGT CTGTCCTGAT TTTGGCAGTA 151 TGTTTCCTCG GCTGGCTCGC CGGTACGGAA GCAGGTTTGC GCTTCGGGCT 201 GTACCAAATC CCGTCTTGGT TCGGCGTAAA CATTTCCTCC CAAAACCTCA 251 AAGGCACGCT GCTCGACGGC TTCGACGGCG ACAACTGGTC GATAGAAACC 301 GAGGGGGCAG ACCTTAAAAT CAGCCGCTTC CGCTTCGCGT GGAAACCGTC 351 CGAACTGATG CGCCGCAGCC TGCACATTAC CGAAATTTCC GCCGGCGACA 401 TCGCCATCGT TACCAAACCG ACTCCGCCTA AAGAAGAACG CCCGCCGCTC 451 AGCCTTCCCG ACAGCATAGA CCTGCCTGCC GCCGTCTATC TCGACCGCTT 501 CGAGACGGGC AAAATCAGCA TGGGCAAAGC CTTTGACAAA CAAACCGTCT 551 ATCTCGAACG GCTGGATGCT TCATACCGTT ACGACCGCAA AGGACACCGC 601 CTTGACCTGA AGGCCGCCGA CACGCCGTGG AGCAGTTCGT CGGGGGCGGC 651 CTCGGTCGGC TTGAAAAAAC CGTTTGCCCT CGATACCGCC ATTTACACCA 701 AAGGCGGACT CGAAGGCAAA ACCATACACA GTACGGCTCG GCTGAGCGGC 751 AGCCTGAAGG ATGTGCGCGC CGAACTGGCG ATCGACGGCG GCAATATCCG 801 CCTCTCGGGA AAATCCGTCA TCCACCCGTT TGCCGAATCA TTGGATAAAA 851 CATTGGAAGA AGTACTGGTC AAAGGGTTCA ACATCAATCC GGCCGCCTTC 901 GTGCCTTCCC TGCCCGATGC CGGACTGAAT TTCGACCTGA CCGCCATCCC 951 GTCGTTTTCA GACGGCATCG CGCTGGAAGG TTCGCTCGAT TTGGAAAACA 1001 CCAAAGCCGG CTTTGCCGAC CGCAACGGCA TCCCCGTCCG TCAGGTTTTA 1051 GGCGGCTTTG TCATCCGGCA GGACGGCACG GTGCATATCG GCAATACGTC 1101 CGCCGCCCTG CTCGGACGGG GCGGCATCAG GCTGTCGGGC AAAATCGACA 1151 CCGAAAAAGA CATCCTCGAT TTAAATATAG GCATCAACTC CGTCGGCGCG 1201 GAAGACGTAC TGCAAACCGC GTTCAAAGGC AGGTTGGACG GCAGCATCGG 1251 CATCGGTGGC ACGACCGCCT CGCCCAAAAT CTCTTGGCAA CTCGGCATCG 1301 GCACGGCGCG CACGGACGGC AGCCTCGCCA TTGCAAGCGA CCCAGCAAAC 1351 GGACAGCGGA AACTGGTGCT CGACACCGTC AACATCGCCG CCGGGCAAGG 1401 CAGCCTGACC GCGCAAGGCT ATCTCGAGCT GTTTAAAGAC CGCCTGCTCA 1451 AGCTGGACAT CCGTTCCCGC GCATTCGACC CTTCGCGCAT CGATCCGCAA 1501 CTTCCGGCAG GCAATATCAA CGGCTCAATA AACCTTGCCG GCGAACTGGC 1551 AAAAGAGAAA TTCACAGGCA AAATGCGGTT TTTACCCGGC ACGTTCAACG 1601 GCGTACCGAT TGCCGGCAGT GCCGACATTG TTTACGAGTC CCGCCACCTT 1651 CCGCGTGCCG CCGTCGATTT GCGGCTGGGG CGGAACATTA TTAAAACAGA 1701 CGGCGGCTTC GGCAAAAAAG GCGACCGGCT TAACCTCAAT ATCACCGCAC 1751 CCGATTTATC CCGTTTCGGT TTCGGACTCG CGGGGTCTTT AAATGTACGC 1801 GGACACCTTT CCGGTGATTT GGACGGCGGC ATCCGAACCT TTGAAACCGA 1851 CCTTTCCGGC GCGGCGCGCA ACCTGCACAT CGGCAAGGCG GCAGACATCC 1901 GTTCGCTCGA TTTCACGCTC AAAGGTTCGC CCGACACAAG CCGCCCGATA 1951 CGCGCCGACA TCAAAGGCAG CCGCCTTTCG CTGTCGGGCG GAGCGGCGGT 2001 TGTCGATACC GCCGACCTGA TGCTGGACGG CACGGGCGTG CAGCACCGCA 2051 TCCGCACACA CGCCGCCATG ACGCTGGATG GCAAACCGTT CAAATTCGAT 2101 TTGGACGCTT CAGGCGGCAT CAACAGGGAA CTTACCCGAT GGAAAGGCAG 2151 CATCGGCATC CTCGACATCG GCGGCGCATT CAACCTCAAG CTGCAAAACC 2201 GTATGACGCT CGAAGCCGGT GCGGAACGCG TGGCGGCAAG TGCGGCAAAT 2251 TGGCAGGCAA TGGGCGGCAG CCTCAACCTG CAACACTTTT CTTGGGATAA 2301 AAAAACCGGC ATATCGGCAA AAGGCGGCGC ACACGGTCTG CATATCGCCG 2351 AGTTGCACAA TTTCTTCAAA CCGCCCTTCG AACACAATCT GGTTTTAAAC 2401 GGCGACTGGG ATGTCGCCTA CGGGCGCAAC GCGCGCGGCT ACCTCAATAT 2451 CAGCCGGCAA AGCGGCGATG CCGTATTGCC CGGCGGGCAG GCTTTGGGTT 2501 TGAACGCATT TTCCCTGAAA ACGCGCTTTC AAAACGACCG CATCGGAATC 2551 CTGCTTGACG GCGGCGCGCG TTTCGGGCGG ATTAACGCCG ATTTGGGCAT 2601 CGCCAACGCC TTCGGCGGCA ATATGGCAAA TGCACCGCTC GGCGGCAGGA 2651 TTACCGCCTC CCTTCCCGAC TTGGGCGCAT TGAAGCCCTT TCTGCCCGCC 2701 GCCGCGCAAA ACATTACCGG CAGCCTGAAT GCCGCCGCGC AAATCGGCGG 2751 ACGGGTAGGC TCTCCGTCCG TCAATGCCGC CGTCAACGGC AGCAGCAACT 2801 ACGGGAAAAT CAACGGCAAC ATCACCGTCG GGCAAAGCCG CTCTTTCGAT 2851 ACCGCGCCTT TGGGCGGCAG GCTCAACCTG ACCGTTGCCG ATGCCGAAGT 2901 ATTCCGCAAC TTCCTACCGG TCGGACAAAC CGTCAAAGGC AGCCTGAATG 2951 CCGCCGTAAC CCTCGGCGGC AGCATCGCCG ATCCGCACTT GGGCGGCAGC 3001 ATCAACGGCG ACAAACTCTA TTACCGCAAC CAAACCCAAG GCATCATCTT 3051 GGACAACGGC TCGCTGCGTT CGCATATCGC GGGCAGGAAA TGGGTAATCG 3101 ACAGCCTGAA ATTCCGGCAC GAAGGGACGG CGGAACTCTC CGGTACGGTC 3151 GGTATGGAAA ACAGCGGACC CGATGTCGAT ATCGGCGCGG TGTTCGACAA 3201 ATACCGCATC CTGTCCCGCC CCAACCGCCG CCTGACGGTT TCCGGCAACA 3251 CCCGCCTGCG CTATTCGCCG CAAAAAGGCA TATCCGTTAC CGGGATGATT 3301 AAAACGGATC AGGGGCTGTT CGGTTCGCAA AAATCCTCGA TGCCGTCCGT 3351 CGGCGACGAT GTCGTCGTAT TAGGCGAAGT CAAAAAAGAG GCGGCGGCAC 3401 CGCTCCCCGT CAATATGAAC CTGACTTTAG ACCTCAATGA CGGCATCCGC 3451 TTCGCCGGCT ACGGCGCGGA CGTTACCATA GGCGGCAAAC TGACCCTGAC 3501 CGCCCAATCG GGCGGAAGCG TACGGGGCGT GGGCACGGTC CGCGTCATCA 3551 AAGGGCGTTA TAAGGCATAC GGGCAGGATT TGGACATTAC CAAAGGCACG 3601 GTCTCCTTTG TCGGCCCGCT CAACGATCCC AACCTCAACA TCCGCGCCGA 3651 ACGCCGCCTT TCCCCCGTCG GTGCGGGCGT GGAAATATTG GGCAGCCTCA 3701 ACAGCCCGCG CATTACGCTG ACGGCAAACG AACCGATGAG TGAAAAAGAC 3751 AAGCTCTCTT GGCTCATCCT CAACCGCGCC GGCAGCGGCA GCAGCGGCGA 3801 CAATGCCGCC CTGTCTGCAG CCGCAGGTGC GCTGCTTGCC GGGCAAATCA 3851 ACGACCGCAT CGGGCTGGTG GATGATTTGG GCTTTACCAG CAAGCGCAGC 3901 CGCAACGCGC AAACCGGCGA ACTCAACCCC GCCGAACAGG TGCTGACCGT 3951 CGGCAAACAA CTGACCGGCA AACTCTACAT CGGCTACGAA TACAGCATCT 4001 CCAGCGCGGA ACAGTCCGTC AAACTGATTT ACCGGCTGAC CCGCGCCATA 4051 CAGGCGGTTG CCCGTATCGG CAGCCGTTCG TCGGGCGGCG AGCTGACATA 4101 CACCATACGT TTCGACCGCT TCTCCGGTTC GGACAAAAAA GACTCCGCCG 4151 GAAACGGCAA AGGAAAATAA This corresponds to the amino acid sequence <SEQ ID 1184; ORF 285>: m285.pep 1 MTDTAPTDTD PTENGTRKMP SEHRPTPPAK KRRPLLKLSA ALLSVLILAV 51 CFLGWLAGTE AGLRFGLYQI PSWFGVNISS QNLKGTLLDG FDGDNWSIET 101 EGADLKISRF RFAWKPSELM RRSLHITEIS AGDIAIVTKP TPPKEERPPL 151 SLPDSIDLPA AVYLDRFETG KISMGKAFDK QTVYLERLDA SYRYDRKGHR 201 LDLKAADTPW SSSSGAASVG LKKPFALDTA IYTKGGLEGK TIHSTARLSG 251 ΞLKDVRAELA IDGGNIRLSG KSVIHPFAES LDKTLEEVLV KGFNINPAAF 301 VPSLPDAGLN FDLTAIPSFS DGIALEGSLD LENTKAGFAD RNGIPVRQVL 351 GGFVIRQDGT VHIGNTSAAL LGRGGIRLSG KIDTEKDILD LNIGINSVGA 401 EDVLQTAFKG RLDGSIGIGG TTASPKISWQ LGIGTARTDG SLAIASDPAN 451 GQRKLVLDTV NIAAGQGSLT AQGYLELFKD RLLKLDIRSR AFDPSRIDPQ 501 LPAGNINGSI NLAGELAKEK FTGKMRFLPG TFNGVPIAGS ADIVYESRHL 551 PRAAVDLRLG RNIIKTDGGF GKKGDRLNLN ITAPDLSRFG FGLAGSLNVR 601 GHLSGDLDGG IRTFETDLSG AARNLHIGKA ADIRSLDFTL KGSPDTSRPI 651 RADIKGSRLS LSGGAAWDT ADLMLDGTGV QHRIRTHAAM TLDGKPFKFD 701 LDASGGINRE LTRWKGSIGI LDIGGAFNLK LQNRMTLEAG AERVAASAAN 751 WQAMGGSLNL QHFSWDKKTG ISAKGGAHGL HIAELHNFFK PPFEHNLVLN 801 GDWDVAYGRN ARGYLNISRQ SGDAVLPGGQ ALGLNAFSLK TRFQNDRIGI 851 LLDGGARFGR INADLGIANA FGGNMANAPL GGRITASLPD LGALKPFLPA 901 AAQNITGSLN AAAQIGGRVG SPSVNAAVNG SSNYGKINGN ITVGQSRSFD 951 TAPLGGRLNL TVADAEVFRN FLPVGQTVKG SLNAAVTLGG SIADPHLGGS 1001 INGDKLYYRN QTQGIILDNG SLRSHIAGRK WVIDSLKFRH EGTAELSGTV 1051 GMENSGPDVD IGAVFDKYRI LSRPNRRLTV SGNTRLRYSP QKGISVTGMI 1101 KTDQGLFGSQ KSSMPSVGDD VWLGEVKKE AAAPLPVNMN LTLDLNDGIR 1151 FAGYGADVTI GGKLTLTAQS GGSVRGVGTV RVIKGRYKAY GQDLDITKGT 1201 VSFVGPLNDP NLNIRAERRL SPVGAGVEIL GSLNSPRITL TANEPMSEKD 1251 KLSWLILNRA GSGSSGDNAA LSAAAGALLA GQINDRIGLV DDLGFTSKRS 1301 RNAQTGELNP AEQVLTVGKQ LTGKLYIGYE YSISSAEQSV KLIYRLTRAI 1351 QAVARIGSRS SGGELTYTIR FDRFSGSDKK DSAGNGKGK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m285/g285 36.5% identity in 1389 aa overlap 10 20 30 40 50 60 m285. pep MTDTAPTDTDPTEΝGTRKMPSEHRPTPPAKKRRPLLKLSAALLSVLILAVCFLGWLAGTE I I I I : I I I I I I I I I I I I I I II I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I g285 MTDTTPTDTDPTEΝGTRKMPSEHRPAPPAKKRRPLLKLSAALLSVLILAVCFLGWIAGTE 10 20 30 40 50 60 70 80 90 100 110 120 m285.pep AGLRFGLYQIPSWFGVΝISSQΝLKGTLLDGFDGDΝWSIETEGADLKISRFRFAWKPSELM I I I I I I I I I I I I I I I I I I I I II I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g285 AGLRFGLYQIPSWFGVΝISSQΝLKGTLLDGFDGDΝWSIETEGADLKISRFRFAWKPSELM 70 80 90 100 110 120 130 140 150 160 170 180 m285.pep RRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPAAVYLDRFETGKISMGKAFDK I I I I I I I : I I I I I I I I I I I I I II I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I: I I I g285 RRSLHITDISAGDIAIVTKPTPPKEERPPQGLPDSIDLPAAVYLDRFETGKISMGKTFDK 130 140 150 160 170 180 190 200 210 220 230 240 m285.pep QTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGAASVGLKKPFALDTAIYTKGGLEGK I I I I I I I I : I : I I I I I I I I I I I I I I I I I I I I I I I I: I I I I I I I I I I I I I I I I I I I I : I I : g285 QTVYLERLΝAAYRYDRKGHRLDLKAADTPWSSSSGSASVGLKKPFALDTAIYTKGGFEGE 190 200 210 220 230 240 250 260 270 280 290 300 m285.pep TIHSTARLSGSLKDVRAELAIDGGΝIRLSGKSVIHPFAESLDKTLEEVLVKGFΝIΝPAAF I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I g285 TIHSTARLSGSLKDVRAELTIDGGNIRLSGKSVIHPFAESLDKTLEEVLVKGFNINPSAF 250 260 270 280 290 300 310 320 330 340 350 360 m285.pep VPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENTKAGFADRNGIPVRQVLGGFVIRQDGT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g285 VPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENTKAGFADRNGIPVRQVLGGFVIRQDGT 310 320 330 340 350 360 370 380 390 400 410 420 m285.pep VHIGNTSAALLGRGGIRLSGKIDTEKDILDLNIGINSVGAEDVLQTAFKGRLDGSIGIGG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g285 VHIGNTSAALLGRGGIRLSGKIDTEKDILDLNIGINSVGAEDVLQTAFKGRLDGSIGIGG 370 380 390 400 410 420 430 440 450 460 470 480 m285.pep TTASPKISWQLGIGTARTDGSLAIASDPANGQRKLVLDTVNIAAGQGSLTAQGYLELFKD I I I I I I I I I I I I lllllllll lllllll I I I I I : I I I I I : I I : I I I I I I I I I I I I I I g285 TTASPKISWQLGTGTARTDGSLPIASDPANEQRKLVFDTVNISAGEGSLTAQGYLELFKD 430 440 450 460 470 480 490 500 510 520 530 540 m285.pep RLLKLDIRSRAFDPSRIDPQLPAGNINGSINLAGELAKEKFTGKMRFLPGTFNGVPIAGS I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g285 RLLKLDIRSRAFDPSRIDPQFPAGNINGSIHLAGELAKEKFTGKMRFLPGTFNGVPIAGS 490 500 510 520 530 540 550 560 570 580 590 600 m285.pep ADIVYESRHLPRAAVDLRLGRNIIKTDGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVR I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g285 ADIVYESRHLPRAAVDLRLGRNIVKTDGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVR 550 560 570 580 590 600 610 620 630 640 650 660 m285.pep GHLSGDLDGGIRTFETDLSGAARNLHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLS I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I : I I I g285 GHLSGDLDGGIRTFETDLSGTARNLHIGKAADIRSLDFTLKGSPGTSRPMRADIKGGRLS 610 620 630 640 650 660 670 680 690 700 710 720 m285 . pep LSGGAAWDTADLMLDGTGVQHRIRTHAAMTLDGKPFKFDLDASGGINRELTRWKGSIGI I I I I I I I I I I I I I : I I I : I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I g285 LSGGAAWDTAGLTLEGTGAQHRIRTHAAMTLDGKPFKLDLDASGGINRELTRWKGSIGI 670 680 690 700 710 720 730 740 750 760 770 780 m285 . pep LDIGGAFNLKLQNRMTLEAGAERVAASAANWQAMGGSLNLQHFSWDKKTGISAKGGAHGL I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I : I I g285 LDIGGAFNLKLQNRMTLEAGAEHVAASAANWQAMGGSLNLQHFSWDRKTGISAKGGARGL 730 740 750 760 770 780 790 800 810 820 830 840 m285. pep HIAELHNFFKPPFEHNLVLNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLK I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g285 HIAELHNFFKPPFEHNLVLNGDWDVAYGHNARGYLNISRQSGDAVLPGGQALGLNAFSLK 790 800 810 820 830 840 850 860 870 880 890 900 m285. pep TRFQNDRIGILLDGGARFGRINADLGIANAFGGNMANAPLGGRITASLPDLGALKPFLPA I I I I I I I I I I I I I I I II I I I I I I I I I I : I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I g285 TRFQNDRIGILLDGGARFGRINADLGIGNAFGGNMANTPLGGRITASLPDLGALKPFLPA 850 860 870 880 890 900 910 920 930 940 950 960 m285.pep AAQNITGSLNAAAQIGGRVGSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNL I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g285 AAQNITGSLNASAQIGGRVGSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNL 910 920 930 940 950 960 970 980 990 1000 1010 1020 m285.pep TVADAEVFRNFLPVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNG I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g285 TVADAEAFRNFLPVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNG 970 980 990 1000 1010 1020 1030 1040 1050 1060 1070 1080 m285.pep SLRSHIAGRKWVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTV I I I I I I I I I I I I I I I I I I I I I I I I I I II I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I g285 SLRSHIAGRKWVIDSLKFRHEGTAELSGTVSMENSVPDVDIGAVFDKYRILSRPNRRLTV 1030 1040 1050 1060 1070 1080 1090 1100 1110 1120 1130 1140 m285. pep SGNTRLRYSPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDWVLGEVKKEAAAPLPVNMN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I llllll g285 SGNTRLRYSPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVWLGEVKKEAAASLPVNMN 1090 1100 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 m285. pep LTLDLNDGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGT I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I g285 LTLDLNDGIRFSGYGADVTIGGKLTLTAQPGGNVRGVGTVRVIKGRYKAYGQDLDITKGT 1150 1160 1170 1180 1190 1200 1210 1220 1230 1240 1250 1260 m285.pep VSFVGPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g285 VSFVGPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRA 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 1310 1320 m285. pep GSGSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g285 GSGSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ 1270 1280 1290 1300 1310 1320 1330 1340 1350 1360 1370 1380 m285.pep LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSDKK I I I I I I I I I I I: I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I g285 LTGKLYIGYEYGISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRLFGSDKK 1330 1340 1350 1360 1370 1380 1390 m285.pep DSAGNGKGKX I I I I I I I I I I g285 DSAGNGKGKX The following partial DNA sequence was identified in N meningitidis <SEQ ID 1185>: a285. seq 1 ATGACCGATA CCGCACCGAC AGATACCGAT CCGACCGAAA ACGGCACGCG 51 CAAAATGCCG TCTGAACACC GCCCTACCCC GCCGGCAAAA AAACGCCGCC 101 CGCTGCTGAA GCTGTCGGCG GCACTGCTGT CTGTTCTGAT TTTGGCAGTA 151 TGTTTCCTCG GCTGGCTCGC CGGCACGGAA GCGGGTTTGC GCTTCGGGCT 201 GTACCAAATC CCGTCTTGGT TCGGCGTAAA CATTTCCTCC CAAAACCTCA 251 AAGGCACGCT GCTCGACGGC TTCGACGGCG ACAACTGGTC GATAGAAACC 301 GAGGGGGCAG ACCTTAAAAT CAGCCGCTTC CGCTTCGCGT GGAAACCGTC 351 CGAACTGATG CGCCGCAGCC TGCACATTAC CGAAATTTCC GCCGGCGACA 401 TCGCCATCGT TACCAAACCG ACTCCGCCTA AAGAAGAACG CCCGCCGCTC 451 AGCCTTCCCG ACAGCATAGA CCTGCCTGCC GCCGTCTATC TCGACCGCTT 501 CGAGACGGGC AAAATCAGCA TGGGCAAAGC CTTTGACAAA CAAACCGTCT 551 ATCTCGAACG GCTGGATGCT TCATACCGTT ACGACCGCAA AGGACACCGC 601 CTCGACCTGA AGGCTGCCGA CACGCCGTGG AGCAGTTCGT CGGGGTCAGC 651 CTCGGTCGGC TTGAAAAAAC CGTTTGCCCT CGATACCGCC ATTTACACCA 701 AAGGCGGACT CGAAGGCAAA ACCATACACA GTACGGCTCG GCTGAGCGGC 751 AGCCTGAAGG ATGTGCGCGC CGAACTGGCG ATCGACGGCG GCAATATCCG 801 CCTCTCGGGA AAATCCGTCA TCCACCCGTT TGCCGAATCA TTGGATAAAA 851 CATTGGAAGA AGTACTGGTC AAAGGGTTCA ACATCAATCC GTCCGCCTTC 901 GTGCCTTCCC TGCCCGATGC CGGGCTGAAT TTCGACCTGA CCGCCATCCC 951 GTCGTTTTCA GACGGCATCG CGCTGGAAGG CTCGCTCGAT TTGGAAAACA 1001 CCAAAGCCGG CTTTGCCGAC CGCAACGGCA TCCCCGTCCG TCAGGTTTTA 1051 GGCAGCTTTG TCATCCGGCA GGACGGCACG GTGCATATCG GCAATACGTC 1101 CGTCGCCCTG CTCGGACGGG GCGGCATCAG GCTGTCGGGC AAAATCGACA 1151 CCGAAAAAGA CATCCTCGAT TTAAATATAG GCATCAACTC CGTCGGCGCG 1201 GAAGACGTAC TGCAAACCGC GTTCAAAGGC AGGTTGGACG GCAGCATCGG 1251 CATCGGTGGC ACGACCGCCT CGCCCAAAAT CTCTTGGCAA CTCGGCATCG 1301 GCACGGCGCG CACGGACGGC AGCCTCGCCA TTGCAAGCGA CCCCGCAAAC 1351 GGACAGCGGA AACTGGTGCT CGACACCGTC AACATCGCCG CCGGGCAAGG 1401 CAGCCTGACC GCGCAAGGCT ATCTCGAGCT GTTTAAAGAC CGCCTGCTCA 1451 AGCTGGACAT CCGTTCCCGC GCATTCGACC CTTCGCGCAT CGATCCGCAA 1501 CTTCCGGCAG GCAATATCAA CGGCTCAATA AACCTTGCCG GCGAACTGGC 1551 AAAAGAGAAA TTCACAGGCA AAATGCGGTT TTTACCCGGC ACGTTCAACG 1601 GCGTACCGAT TGCCGGCAGT GCCGACATTG TTTACGAGTC CCGCCACCTT 1651 CCGCGTGCCG CCGTCGATTT GCGGCTGGGG CGGAACATTA TTAAAACAGA 1701 CGGCGGCTTC GGCAAAAAAG GCGACCGGCT TAACCTCAAT ATCACCGCAC 1751 CCGATTTATC CCGTTTCGGT TTCGGACTCG CGGGGTCTTT AAATGTACGC 1801 GGACACCTTT CCGGCGATTT GGACGGTGGC ATCCGAACCT TTGAAACCGA 1851 CCTTTCCGGC GCGGCGCGCA ACCTGCACAT CGGCAAGGCG GCAGACATCC 1901 GTTCGCTCGA TTTCACGCTC AAAGGTTCGC CCGACACAAG CCGCCCGATA 1951 CGCGCCGACA TCAAAGGCAG CCGCCTTTCG CTGTCGGGCG GAGCGGAGGT 2001 TGTCGATACC GCCGACCTGA TGCTGGACGG CACGGGCGTG CAGCACCGCA 2051 TCCGCACACA CGCCGCCATG ACGCTGGATG GCAAACCGTT CAAATTCGAT 2101 TTGGACGCTT CAGGCGGCAT CAACAGGGAA CTTACCCGAT GGAAAGGCAG 2151 CATCGGCATC CTCGACATCG GCGGCGCATT CAACCTCAAG CTGCAAAACC 2201 GTATGACGCT CGAAGCCGGT GCGGAACGCG TGGCGGCAAG TGCGGCAAAT 2251 TGGCAGGCAA TGGGCGGCAG CCTCAACCTG CAACACTTTT CTTGGGATAA 2301 AAAAACCGGC ATATCGGCAA AAGGCGGCGC ACACGGTCTG CATATCGCCG 2351 AGTTGCACAA TTTCTTCAAA CCGCCCTTCG AACACAATCT GGTTTTAAAC 2401 GGCGACTGGG ATGTCGCCTA CGGGCGAAAC GCGCGCGGCT ACCTCAATAT 2451 CAGCCGGCAA AGCGGCGATG CCGTATTGCC CGGCGGGCAG GCTTTGGGTT 2501 TGAACGCATT TTCCCTGAAA ACGCGCTTTC AAAACGACCG TATCGGAATC 2551 CTGCTTGACG GCGGCGCGCG TTTCGGGCGG ATTAACGCCG ATTTGGACAT 2601 CGGCAACGCC TTCGGCGGCA ATATGGCAAA TGCACCGCTC GGCGGCAGGA 2651 TTACCGCCTC CCTTCCCGAC TTGGGCACAT TGAAGCCCTT TCTGCCCGCC 2701 GCCGCGCAAA ACATTACCGG CAGCCTGAAT GCCGCCGCGC AAATCGGCGG 2751 ACGGGTCGGC TCTCCGTCCG TCAATGCCGC CGTCAACGGC AGCAGCAACT 2801 ACGGGAAAAT CAACGGCAAC ATCACCGTCG GGCAAAGCCG CTCTTTCGAT 2851 ACCGCGCCTT TGGGCGGCAG GCTCAACCTG ACCGTTGCCG ATGCCGAAGT 2901 ATTCCGCAAC TTCCTACCGG TCGGACAAAC CGTCAAAGGC AGCCTGAATG 2951 CCGCCGTAAC CCTCGGCGGC AGCATCGCCG ATCCGCACTT GGGCGGCAGC 3001 ATCAACGGCG ACAAACTCTA TTACCGCAAC CAAACCCAAG GCATCATCTT 3051 GGACAACGGC TCGCTGCGTT CGCATATCGC GGGCAGGAAA TGGGTAATCG 3101 ACAGCCTGAA ATTCCGGCAC GAAGGGACGG CGGAACTCTC CGGTACGGTC 3151 GGTATGGAAA ACAGCGGACC CGATGTCGAT ATCGGCGCGG TGTTCGACAA 3201 ATACCGCATC CTGTCCCGCC CCAACCGCCG CCTGACGGTT TCCGGCAACA 3251 CCCGCCTGCG CTATTCGCCG CAAAAAGGCA TATCCGTTAC CGGGATGATT 3301 AAAACGGATC AGGGGCTGTT CGGTTCGCAA AAATCCTCGA TGCCGTCCGT 3351 CGGCGACGAT GTCGTCGTAT TAGGCGAAGT CAAAAAAGAG GCGGCGGCAC 3401 CGCTCCCCGT CAATATGAAC CTGACTTTAG ACCTCAATGA CGGCATCCGC 3451 TTCGCCGGCT ACGGCGCGGA CGTTACCATA GGCGGCAAAC TGACCCTGAC 3501 CGCCCAATCG GGCGGAAGCG TGCGGGGCGT GGGCACGGTC CGCGTCATCA 3551 AAGGGCGTTA TAAGGCATAC GGGCAGGATT TGGACATTAC CAAAGGCACG 3601 GTCTCCTTTG TCGGCCCGCT CAACGACCCC AACCTCAACA TCCGCGCCGA 3651 ACGCCGCCTT TCCCCCGTCG GTGCGGGCGT GGAAATATTG GGCAGCCTCA 3701 ACAGTCCGCG CATTACGCTG ACGGCAAACG AACCGATGAG TGAAAAAGAC 3751 AAGCTCTCCT GGCTCATCCT CAACCGCGCC GGCAGTGGCA GCAGCGGCGA 3801 CAATGCCGCC CTGTCCGCAG CCGCCGGCGC GCTGCTTGCC GGGCAAATCA 3851 ACGACCGCAT CGGGCTGGTG GATGATTTGG GCTTTACCAG CAAGCGCAGC 3901 CGCAACGCGC AAACCGGCGA ACTCAACCCC GCCGAACAGG TGCTGACCGT 3951 CGGCAAACAA CTGACCGGCA AACTCTACAT CGGCTACGAA TACAGCATCT 4001 CCAGCGCGGA ACAGTCCGTC AAACTGATTT ACCGGCTGAC CCGCGCCATA 4051 CAGGCGGTTG CCCGTATCGG CAGCCGTTCG TCGGGCGGCG AGCTGACATA 4101 CACCATACGT TTCGACCGCT TCTCCGGTTC GGACAAAAAA GACTCCGCCG 151 GAAACAGCAA AGGAAAATAA This corresponds to the amino acid sequence <SEQ ID 1186; ORF 285. a>: a285.pep 1 MTDTAPTDTD PTENGTRKMP SEHRPTPPAK KRRPLLKLSA ALLSVLILAV 51 CFLGWLAGTE AGLRFGLYQI PSWFGVNISS QNLKGTLLDG FDGDNWSIET 101 EGADLKISRF RFAWKPSELM RRSLHITEIS AGDIAIVTKP TPPKEERPPL 151 SLPDSIDLPA AVYLDRFETG KISMGKAFDK QTVYLERLDA SYRYDRKGHR 201 LDLKAADTPW SSSSGSASVG LKKPFALDTA IYTKGGLEGK TIHSTARLSG 251 SLKDVRAELA IDGGNIRLSG KSVIHPFAES LDKTLEEVLV KGFNINPSAF 301 VPSLPDAGLN FDLTAIPSFS DGIALEGSLD LENTKAGFAD RNGIPVRQVL 351 GSFVIRQDGT VHIGNTSVAL LGRGGIRLSG KIDTEKDILD LNIGINSVGA 401 EDVLQTAFKG RLDGSIGIGG TTASPKISWQ LGIGTARTDG SLAIASDPAN 451 GQRKLVLDTV NIAAGQGSLT AQGYLELFKD RLLKLDIRSR AFDPSRIDPQ 501 LPAGNINGSI NLAGELAKEK FTGKMRFLPG TFNGVPIAGS ADIVYESRHL 551 PRAAVDLRLG RNIIKTDGGF GKKGDRLNLN ITAPDLSRFG FGLAGSLNVR 601 GHLSGDLDGG IRTFETDLSG AARNLHIGKA ADIRSLDFTL KGSPDTSRPI 651 RADIKGSRLS LSGGAEWDT ADLMLDGTGV QHRIRTHAAM TLDGKPFKFD 701 LDASGGINRE LTRWKGSIGI LDIGGAFNLK LQNRMTLEAG AERVAASAAN 751 WQAMGGSLNL QHFSWDKKTG ISAKGGAHGL HIAELHNFFK PPFEHNLVLN 801 GDWDVAYGRN ARGYLNISRQ SGDAVLPGGQ ALGLNAFSLK TRFQNDRIGI 851 LLDGGARFGR INADLDIGNA FGGNMANAPL GGRITASLPD LGTLKPFLPA 901 AAQNITGSLN AAAQIGGRVG SPSVNAAVNG SSNYGKINGN ITVGQSRSFD 951 TAPLGGRLNL TVADAEVFRN FLPVGQTVKG SLNAAVTLGG SIADPHLGGS 1001 INGDKLYYRN QTQGIILDNG SLRSHIAGRK WVIDSLKFRH EGTAELSGTV 1051 GMENSGPDVD IGAVFDKYRI LSRPNRRLTV SGNTRLRYSP QKGISVTGMI 1101 KTDQGLFGSQ KSSMPSVGDD VWLGEVKKE AAAPLPVNMN LTLDLNDGIR 1151 FAGYGADVTI GGKLTLTAQS GGSVRGVGTV RVIKGRYKAY GQDLDITKGT 1201 VSFVGPLNDP NLNIRAERRL SPVGAGVEIL GSLNSPRITL TANEPMSEKD 1251 KLSWLILNRA GSGSSGDNAA LSAAAGALLA GQINDRIGLV DDLGFTSKRS 1301 RNAQTGELNP AEQVLTVGKQ LTGKLYIGYE YSISSAEQSV KLIYRLTRAI 1351 QAVARIGSRS SGGELTYTIR FDRFSGSDKK DSAGNSKGK* m285/a285 99.4% identity in 1389 aa overlap 10 20 30 40 50 60 m285.pep MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRPLLKLSAALLSVLILAVCFLGWLAGTE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a285 MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRPLLKLSAALLSVLILAVCFLGWLAGTE 10 20 30 40 50 60 70 80 90 100 110 120 m285.pep AGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEGADLKISRFRFAWKPSELM I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a285 AGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEGADLKISRFRFAWKPSELM 70 80 90 100 110 120 130 140 150 160 170 180 m285.pep RRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPAAVYLDRFETGKISMGKAFDK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I II I I I I I I a285 RRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPAAVYLDRFETGKISMGKAFDK 130 140 150 160 170 180 190 200 210 220 230 240 m285. pep QTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGAASVGLKKPFALDTAIYTKGGLEGK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I a285 QTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGSASVGLKKPFALDTAIYTKGGLEGK 190 200 210 220 230 240 250 260 270 280 290 300 m285.pep TIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHPFAESLDKTLEEVLVKGFNINPAAF I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I a285 TIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHPFAESLDKTLEEVLVKGFNINPSAF 250 260 270 280 290 300 310 320 330 340 350 360 m285.pep VPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENTKAGFADRNGIPVRQVLGGFVIRQDGT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I a285 VPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENTKAGFADRNGIPVRQVLGSFVIRQDGT 310 320 330 340 350 360 370 380 390 400 410 420 m285.pep VHIGNTSAALLGRGGIRLSGKIDTEKDILDLNIGINSVGAEDVLQTAFKGRLDGSIGIGG I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a285 VHIGNTSVALLGRGGIRLSGKIDTEKDILDLNIGINSVGAEDVLQTAFKGRLDGSIGIGG 370 380 390 400 410 420 430 440 450 460 470 480 m285.pep TTASPKISWQLGIGTARTDGSLAIASDPANGQRKLVLDTVNIAAGQGSLTAQGYLELFKD I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a285 TTASPKISWQLGIGTARTDGSLAIASDPANGQRKLVLDTVNIAAGQGSLTAQGYLELFKD 430 440 450 460 470 480 490 500 510 520 530 540 m285.pep RLLKLDIRSRAFDPSRIDPQLPAGNINGSINLAGELAKEKFTGKMRFLPGTFNGVPIAGS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a285 RLLKLDIRSRAFDPSRIDPQLPAGNINGSINLAGELAKEKFTGKMRFLPGTFNGVPIAGS 490 500 510 520 530 540 550 560 570 580 590 600 m285.pep ADIVYESRHLPRAAVDLRLGRNIIKTDGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a285 ADIVYESRHLPRAAVDLRLGRNIIKTDGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVR 550 560 570 580 590 600 610 620 630 640 650 660 m285.pep GHLSGDLDGGIRTFETDLSGAARNLHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLS I I I I I I I I I I I I I I I I I I I I I II I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I a285 GHLSGDLDGGIRTFETDLSGAARNLHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLS 610 620 630 640 650 660 670 680 690 700 710 720 m285 . pep LSGGAAWDTADLMLDGTGVQHRIRTHAAMTLDGKPFKFDLDASGGINRELTRWKGSIGI I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a285 LSGGAEWDTADLMLDGTGVQHRIRTHAAMTLDGKPFKFDLDASGGINRELTRWKGSIGI 670 680 690 700 710 720 730 740 750 760 770 780 m285 . pep LDIGGAFNLKLQNRMTLEAGAERVAASAANWQAMGGSLNLQHFSWDKKTGISAKGGAHGL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a285 LDIGGAFNLKLQNRMTLEAGAERVAASAANWQAMGGSLNLQHFSWDKKTGISAKGGAHGL 730 740 750 760 770 780 790 800 810 820 830 840 m285. pep HIAELHNFFKPPFEHNLVLNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a285 HIAELHNFFKPPFEHNLVLNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLK 790 800 810 820 830 840 850 860 870 880 890 900 m285. pep TRFQNDRIGILLDGGARFGRINADLGIANAFGGNMANAPLGGRITASLPDLGALKPFLPA I I I I I I I II I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I: I I I I I I I a285 TRFQNDRIGILLDGGARFGRINADLDIGNAFGGNMANAPLGGRITASLPDLGTLKPFLPA 850 860 870 880 890 900 910 920 930 940 950 960 m285.pep AAQNITGSLNAAAQIGGRVGSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a285 AAQNITGSLNAAAQIGGRVGSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNL 910 920 930 940 950 960 970 980 990 1000 1010 1020 m285.pep TVADAEVFRNFLPVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I a285 TVADAEVFRNFLPVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNG 970 980 990 1000 1010 1020 1030 1040 1050 1060 1070 1080 m285.pep SLRSHIAGRKWVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a285 SLRSHIAGRKWVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTV 1030 1040 1050 1060 .1070 1080 1090 1100 1110 1120 1130 1140 m285. pep SGNTRLRYSPQKGIΞVTGMIKTDQGLFGSQKSSMPSVGDDVWLGEVKKEAAAPLPVNMN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a285 SGNTRLRYSPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVWLGEVKKEAAAPLPVNMN 1090 1100 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 m285. pep LTLDLNDGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I a285 LTLDLNDGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGT 1150 1160 1170 1180 1190 1200 1210 1220 1230 1240 1250 1260 m285.pep VSFVGPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I a285 VSFVGPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRA 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 1310 1320 m285.pep GSGSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a285 GSGSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ 1270 1280 1290 1300 1310 1320 1330 1340 1350 1360 1370 1380 m285.pep LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSDKK I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a285 LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSDKK 1330 1340 1350 1360 1370 1380 1390 m285.pep DSAGNGKGKX I I I I I: I I I I a285 DSAGNSKGKX 1390 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1187>: g285-l.seq 1 CTGAAGCTGT CGGCGGCACT GCTGTCTGTC CTGATTTTGG CAGTATGTTT 51 CCTCGGCTGG ATCGCCGGTA CGGAAGCAGG TTTGCGCTTC GGGCTGTACC 101 AAATCCCGTC CTGGTTCGGC GTAAACATTT CCTCCCAAAA CCTCAAAGGC 151 ACACTGCTCG ACGGCTTCGA CGGCGACAAC TGGTCGATAG AAACCGAGGG 201 GGCAGACCTT AAAATCAGCC GCTTCCGCTT CGCGTGGAAA CCGTCCGAAC 251 TGATGCGCCG CAGCCTGCAC ATCACCGACA TCTCCGCCGG CGACATCGCC 301 ATCGTAACCA AACCGACTCC GCCTAAAGAA GAACGCCCGC CTCAAGGCCT 351 GCCCGACAGC ATAGACCTGC CCGCCGCCGT CTATCTCGAC CGCTTCGAGA 401 CGGGCAAAAT CAGCATGGGC AAAACCTTTG ACAAACAAAC CGTCTATCTC 451 GAACGCCTCA ACGCGGCATA CCGTTACGAC CGTAAAGGGC ACCGCCTCGA 501 CCTGAAGGCC GCCGACACGC CGTGGAGCAG TTCGTCGGGG TCAGCCTCGG 551 TCGGCTTGAA AAAACCGTTT GCCCTCGATA CCGCCATTTA CACCAAAGGC 601 GGATTCGAAG GCGAAACCAT ACACAGTACG GCGCGGCTGA GCGGCAGCCT 651 GAAGGATGTG CGCGCCGAAC TGACGATCGA CGGCGGCAAT ATCCGCCTCT 701 CGGGAAAATC CGTCATCCAC CCGTTTGCCG AATCATTGGA TAAAACATTG 751 GAAGAAGTAC TGGTCAAAGG ATTCAACATC AATCCGTCCG CCTTCGTGCC 801 TTCCCTGCCC GATGCCGGGC TGAATTTCGA CCTGACCGCC ATCCCGTCGT 851 TTTCAGACGG CATCGCGCTG GAAGGCTCGC TCGATTTGGA AAACACCAAA 901 GCCGGCTTTG CCGACCGCAA CGGCATCCCC GTCCGTCAGG TTTTGGGCGG 951 CTTTGTCATC CGGCAGGACG GCACGGTGCA TATCGGCAAT ACGTCCGCCG 1001 CCCTGCTCGG ACGGGGCGGC ATCAGGCTGT CGGGCAAAAT CGACACCGAA 1051 AAAGACATCC TTGATTTAAA TATAGGCATC AACTCCGTCG GCGCGGAAGA 1101 CGTGCTGCAA ACCGCGTTCA AAGGCAGGTT GGACGGCAGC ATCGGCATCG 1151 GCGGCACGAC CGCCTCGCCC AAAATCTCTT GGCAACTCGG CACCGGCACG 1201 GCACGCACGG ACGGCAGCCt cgcCATCGCA AGCGAcCCCG CAAACGAACA 1251 GCGGAAACTG GTGTTCGACA CCGTCAACAT CTCCGCCGGG GAAGGCAGCC 1301 TGACCGCGCA AGGCTATCTC GAGCTGTTTA AAGACCGCCT GCTCAAGCTG 1351 GACATCCGTT CCCGCGCATT CGACCCTTCG CGCATCGATC CGCAATTTCC 1401 GGCAGGCgat atCAACGGTT CGATTCATCT TGCCGGTGAA CTGGCAAAAG 1451 AGAAATTTAC GGGCAAAATG CGTTTTTTGC CCGGTACGTT CAACGGCGTG 1501 CCGATTGCCG GCAGCGCCGA CATTGTTTAC GAGTCCCGCC ACCTTCCGCG 1551 CGCCGCCGTC GATTTGCGGT TGGGGCGGAA CATCGTCAAA ACAGACGGCG 1601 GCTTCGGCAA AAAAGGCGAC CGGCTTAACC TCAATATCAC CGCACCCGAT 1651 TTATCCCGTT TCGGTTTCGG ACTCGCGGGG TCTTTAAATG TACGCGGACA 1701 CCTTTCCGGC GATTTGGACG GCGGCATCCG AACCTTTGAA ACCGACCTTT 1751 CCGGCACGGC GCGCAACTTA CACATCGGCA AAGCGGCAGA CATCCGTTCG 1801 CTCGATTTTA CCCTCAAAGG CTCACCCGGC ACAAGCCGCC CGATGCGCGC 1851 CGATATCAAG GGCGGCCGCC TTTCCCTGTC GGGCGGCGCG GCGGTTGTCG 1901 ATACCGCCGG CCTGACGCTG GAAGGTACGG GCGCGCAGCA CCGCATCCGC 1951 ACACACGCCG CCATGACGCT GGACGGCAAA CCGTTCAAAC TCGATTTGGA 2001 CGCTTCAGGC GGCATCAACA GGGAACTTAC CCGATGGAAA GGCAGCATCG 2051 GCATCCTCGA CATCGGCGGC GCATTCAACC TCAAGCTGCA AAACCGTATG 2101 ACGCTCGAAG CCGGTGCGGA ACACGTGGCG GCAAGTGCGG CAAATTGGCA 2151 GGCAATGGGC GGCAGCCTCA ACCTGCAACA CTTTTCTTGG GACAGGAAAA 2201 CCGGCATATC GGCAAAAGGC GGCGCACGCG GCCTGCACAT CGCCGAGTTG 2251 CACAATTTCT TCAAACCGCC CTTCGAACAC AATCTGGTTT TAAACGGCGA 2301 CTGGGATGTC GCCTACGGGC ACAACGCGCG CGGCTACCTC AATATCAGCC 2351 GGCAAAGCGG CGATGCCGTA TTGCCCGGCG GGCAGGCTTT GGGTTTGAAC 2401 GCATTTTCCC TGAAAACGCG CTTTCAAAAC GACCGCATCG GAATCCTGCT 2451 TGACGGCGGC GCGCGTTTCG GACGGATTAA CGCCGATTTG GGCATCGGCA 2501 ACGCCTTCGG CGGCAATATG GCAAATACAC CGCTCGGCGG CAGGATTACA 2551 GCCTCCCTTC CCGACTTGGG CGCATTGAAG CCCTTTCTGC CCGCCGCCGC 2601 GCAAAACATT ACCGGCAGCC TGAATGCCTC CGCGCAAATC GGCGGACGGG 2651 TAGGCTCTCC GTCCGTCAAT GCCGCCGTCA ACGGTAGCAG CAACTACGGG 2701 AAAATCAACG GCAATATCAC CGTCGGGCAA AGCCGCTCCT TCGATACCGC 2751 ACCTTTGGGC GGCAGGCTCA ACCTGACCGT TGCCGATGCC GAAGCATTCC 2801 GCAACTTCCT ACCGGTCGGA CAAACCGTCA AAGGCAGCCT GAATGCCGCC 2851 GTAACCCTCG GCGGCAGCAT CGCCGACCCG CACTTGGGCG GCAGTATCAA 2901 CGGCGACAAG CTCTATTACC GCAACCAAAC CCAAGGCATC ATCTTGGACA 2951 ACGGCTCGCT GCGTTCGCAT ATTGCAGGCA GGAAATGGGT AATCGACAGC 3001 CTGAAATTCC GGCACGAAGG GACGGCGGAA CTCTCCGGCA CGGTCAGCAT 3051 GGAAAACAGC GTGCCCGATG TCGATATCGG CGCGGTGTTC GACAAATACC 3101 GCATCCTGTC CCGCCCCAAC CGCCGCCTGA CGGTTTCCGG CAACACCCGC 3151 CTGCGCTATT CGCCGCAAAA AGGCATATCC GTTACCGGTA TGATTAAAAC 3201 TGATCAGGGG CTGTTCGGTT CGCAAAAATC CTCGATGCCG TCCGTCGGCG 3251 ACGATGTCGT CGTATTGGGC GAAGTCAAGA AAGAGGCGGC GGCATCGCTC 3301 CCCGTCAATA TGAACCTGAC TTTAGACCTC AATGACGGCA TCCGCTTCTC 3351 CGGCTACGGC GCGGACGTTA CCATAGGCGG CAAACTGACC CTGACCGCGC 3401 AACCGGGCGG AAATGTGCGT GGGGTGGGCA CGGTCCGCGT CATCAAAGGG 3451 CGTTACAAAG CATACGGGCA GGATTTAGAC ATTACCAAAG GCACAGTCTC 3501 CTTTGTCGGC CCGCTCAACG ACCCCAACCT GAACATCCGC GCCGAACGCC 3551 GCCTTTCCCC CGTCGGTGCG GGCGTGGAAA TATTGGGCAG CCTCAACAGC 3601 CCGCGCATTA CGCTGACGGC AAACGAACCG ATGAGTGAAA AAGACAAGCT 3651 CTCCTGGCTC ATCCTCAACC GTGCCGGCAG CGGCAGCAGC GGCGACAATG 3701 CCGCCCTGTC CGCAGCCGCA GGCGCGCTGC TTGCCGGGCA AATCAACGAC 3751 CGCATCGGGC TGGTGGATGA TTTGGGCTTT ACCAGCAAGC GCAGCCGCAA 3801 CGCGCAAACC GGCGAACTCA ACCCCGCCGA ACAGGTGCTG ACCGTCGGCA 3851 AACAACTGAC CGGCAAACTC TACATCGGCT ACGAATACGG CATCTCCAGC 3901 GCGGAACAGT CCGTCAAACT GATTTACCGG CTGACCCGCG CCATACAGGC 3951 GGTTGCCCGT ATCGGCAGCC GTTCGTCGGG CGGCGAGCTG ACATACACCA 4001 TACGTTTCGA CCGCCTCTTC GGTTCGGACA AAAAAGACTC CGCAGGAAAC 4051 GGCAAAGGGA AATAA This corresponds to the amino acid sequence <SEQ ID 1188; ORF 285-l.ng>: g285-l .pep 1 LKLSAALLSV LILAVCFLGW IAGTEAGLRF GLYQIPSWFG VNIΞSQNLKG 51 TLLDGFDGDN WSIETEGADL KISRFRFAWK PSELMRRSLH ITDISAGDIA 101 IVTKPTPPKE ERPPQGLPDS IDLPAAVYLD RFETGKISMG KTFDKQTVYL 151 ERLNAAYRYD RKGHRLDLKA ADTPWΞSSSG SASVGLKKPF ALDTAIYTKG 201 GFEGETIHST ARLSGΞLKDV RAELTIDGGN IRLSGKSVIH PFAESLDKTL 251 EEVLVKGFNI NPSAFVPSLP DAGLNFDLTA IPSFSDGIAL EGSLDLENTK 301 AGFADRNGIP VRQVLGGFVI RQDGTVHIGN TSAALLGRGG IRLSGKIDTE 351 KDILDLNIGI NSVGAEDVLQ TAFKGRLDGS IGIGGTTASP KISWQLGTGT 401 ARTDGSLAIA SDPANEQRKL VFDTVNISAG EGΞLTAQGYL ELFKDRLLKL 451 DIRSRAFDPS RIDPQFPAGD INGSIHLAGE LAKEKFTGKM RFLPGTFNGV 501 PIAGSADIVY ESRHLPRAAV DLRLGRNIVK TDGGFGKKGD RLNLNITAPD 551 LSRFGFGLAG SLNVRGHLSG DLDGGIRTFE TDLSGTARNL HIGKAADIRS 601 LDFTLKGSPG TSRPMRADIK GGRLSLSGGA AVVDTAGLTL EGTGAQHRIR 651 THAAMTLDGK PFKLDLDASG GINRELTRWK GSIGILDIGG AFNLKLQNRM 701 TLEAGAEHVA ASAANWQAMG GΞLNLQHFSW DRKTGISAKG GARGLHIAEL 751 HNFFKPPFEH NLVLNGDWDV AYGHNARGYL NISRQSGDAV LPGGQALGLN 801 AFSLKTRFQN DRIGILLDGG ARFGRINADL GIGNAFGGNM ANTPLGGRIT 851 ASLPDLGALK PFLPAAAQNI TGSLNASAQI GGRVGSPSVN AAVNGΞSNYG 901 KINGNITVGQ SRSFDTAPLG GRLNLTVADA EAFRNFLPVG QTVKGΞLNAA 951 VTLGGSIADP HLGGSINGDK LYYRNQTQGI ILDNGSLRSH IAGRKWVIDS 1001 LKFRHEGTAE LSGTVΞMENS VPDVDIGAVF DKYRILSRPN RRLTVSGNTR 1051 LRYSPQKGIS VTGMIKTDQG LFGSQKSSMP SVGDDVVVLG EVKKEAAASL 1101 PVNMNLTLDL NDGIRFSGYG ADVTIGGKLT LTAQPGGNVR GVGTVRVIKG 1151 RYKAYGQDLD ITKGTVSFVG PLNDPNLNIR AERRLSPVGA GVEILGSLNS 1201 PRITLTANEP MSEKDKLSWL ILNRAGSGSS GDNAALSAAA GALLAGQIND 1251 RIGLVDDLGF TSKRSRNAQT GELNPAEQVL TVGKQLTGKL YIGYEYGISS 1301 AEQSVKLIYR LTRAIQAVAR IGSRSSGGEL TYTIRFDRLF GSDKKDSAGN 1351 GKGK* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1189>: m285-l.seq 1 CTGAAGCTGT CGGCGGCACT GCTGTCTGTC CTGATTTTGG CAGTATGTTT 51 CCTCGGCTGG CTCGCCGGTA CGGAAGCAGG TTTGCGCTTC GGGCTGTACC 101 AAATCCCGTC TTGGTTCGGC GTAAACATTT CCTCCCAAAA CCTCAAAGGC 151 ACGCTGCTCG ACGGCTTCGA CGGCGACAAC TGGTCGATAG AAACCGAGGG 201 GGCAGACCTT AAAATCAGCC GCTTCCGCTT CGCGTGGAAA CCGTCCGAAC 251 TGATGCGCCG CAGCCTGCAC ATTACCGAAA TTTCCGCCGG CGACATCGCC 301 ATCGTTACCA AACCGACTCC GCCTAAAGAA GAACGCCCGC CGCTCAGCCT 351 TCCCGACAGC ATAGACCTGC CTGCCGCCGT CTATCTCGAC CGCTTCGAGA 401 CGGGCAAAAT CAGCATGGGC AAAGCCTTTG ACAAACAAAC CGTCTATCTC 451 GAACGGCTGG ATGCTTCATA CCGTTACGAC CGCAAAGGAC ACCGCCTTGA 501 CCTGAAGGCC GCCGACACGC CGTGGAGCAG TTCGTCGGGG GCGGCCTCGG 551 TCGGCTTGAA AAAACCGTTT GCCCTCGATA CCGCCATTTA CACCAAAGGC 601 GGACTCGAAG GCAAAACCAT ACACAGTACG GCTCGGCTGA GCGGCAGCCT 651 GAAGGATGTG CGCGCCGAAC TGGCGATCGA CGGCGGCAAT ATCCGCCTCT 701 CGGGAAAATC CGTCATCCAC CCGTTTGCCG AATCATTGGA TAAAACATTG 751 GAAGAAGTAC TGGTCAAAGG GTTCAACATC AATCCGGCCG CCTTCGTGCC 801 TTCCCTGCCC GATGCCGGAC TGAATTTCGA CCTGACCGCC ATCCCGTCGT 851 TTTCAGACGG CATCGCGCTG GAAGGTTCGC TCGATTTGGA AAACACCAAA 901 GCCGGCTTTG CCGACCGCAA CGGCATCCCC GTCCGTCAGG TTTTAGGCGG 951 CTTTGTCATC CGGCAGGACG GCACGGTGCA TATCGGCAAT ACGTCCGCCG 1001 CCCTGCTCGG ACGGGGCGGC ATCAGGCTGT CGGGCAAAAT CGACACCGAA 1051 AAAGACATCC TCGATTTAAA TATAGGCATC AACTCCGTCG GCGCGGAAGA 1101 CGTACTGCAA ACCGCGTTCA AAGGCAGGTT GGACGGCAGC ATCGGCATCG 1151 GTGGCACGAC CGCCTCGCCC AAAATCTCTT GGCAACTCGG CATCGGCACG 1201 GCGCGCACGG ACGGCAGCCT CGCCATTGCA AGCGACCCAG CAAACGGACA 1251 GCGGAAACTG GTGCTCGACA CCGTCAACAT CGCCGCCGGG CAAGGCAGCC 1301 TGACCGCGCA AGGCTATCTC GAGCTGTTTA AAGACCGCCT GCTCAAGCTG 1351 GACATCCGTT CCCGCGCATT CGACCCTTCG CGCATCGATC CGCAACTTCC 1401 GGCAGGCAAT ATCAACGGCT CAATAAACCT TGCCGGCGAA CTGGCAAAAG 1451 AGAAATTCAC AGGCAAAATG CGGTTTTTAC CCGGCACGTT CAACGGCGTA 1501 CCGATTGCCG GCAGTGCCGA CATTGTTTAC GAGTCCCGCC ACCTTCCGCG 1551 TGCCGCCGTC GATTTGCGGC TGGGGCGGAA CATTATTAAA ACAGACGGCG 1601 GCTTCGGCAA AAAAGGCGAC CGGCTTAACC TCAATATCAC CGCACCCGAT 1651 TTATCCCGTT TCGGTTTCGG ACTCGCGGGG TCTTTAAATG TACGCGGACA 1701 CCTTTCCGGT GATTTGGACG GCGGCATCCG AACCTTTGAA ACCGACCTTT 1751 CCGGCGCGGC GCGCAACCTG CACATCGGCA AGGCGGCAGA CATCCGTTCG 1801 CTCGATTTCA CGCTCAAAGG TTCGCCCGAC ACAAGCCGCC CGATACGCGC 1851 CGACATCAAA GGCAGCCGCC TTTCGCTGTC GGGCGGAGCG GCGGTTGTCG 1901 ATACCGCCGA CCTGATGCTG GACGGCACGG GCGTGCAGCA CCGCATCCGC 1951 ACACACGCCG CCATGACGCT GGATGGCAAA CCGTTCAAAT TCGATTTGGA 2001 CGCTTCAGGC GGCATCAACA GGGAACTTAC CCGATGGAAA GGCAGCATCG 2051 GCATCCTCGA CATCGGCGGC GCATTCAACC TCAAGCTGCA AAACCGTATG 2101 ACGCTCGAAG CCGGTGCGGA ACGCGTGGCG GCAAGTGCGG CAAATTGGCA 2151 GGCAATGGGC GGCAGCCTCA ACCTGCAACA CTTTTCTTGG GATAAAAAAA 2201 CCGGCATATC GGCAAAAGGC GGCGCACACG GTCTGCATAT CGCCGAGTTG 2251 CACAATTTCT TCAAACCGCC CTTCGAACAC AATCTGGTTT TAAACGGCGA 2301 CTGGGATGTC GCCTACGGGC GCAACGCGCG CGGCTACCTC AATATCAGCC 2351 GGCAAAGCGG CGATGCCGTA TTGCCCGGCG GGCAGGCTTT GGGTTTGAAC 2401 GCATTTTCCC TGAAAACGCG CTTTCAAAAC GACCGCATCG GAATCCTGCT 2451 TGACGGCGGC GCGCGTTTCG GGCGGATTAA CGCCGATTTG GGCATCGCCA 2501 ACGCCTTCGG CGGCAATATG GCAAATGCAC CGCTCGGCGG CAGGATTACC 2551 GCCTCCCTTC CCGACTTGGG CGCATTGAAG CCCTTTCTGC CCGCCGCCGC 2601 GCAAAACATT ACCGGCAGCC TGAATGCCGC CGCGCAAATC GGCGGACGGG 2651 TAGGCTCTCC GTCCGTCAAT GCCGCCGTCA ACGGCAGCAG CAACTACGGG 2701 AAAATCAACG GCAACATCAC CGTCGGGCAA AGCCGCTCTT TCGATACCGC 2751 GCCTTTGGGC GGCAGGCTCA ACCTGACCGT TGCCGATGCC GAAGTATTCC 2801 GCAACTTCCT ACCGGTCGGA CAAACCGTCA AAGGCAGCCT GAATGCCGCC 2851 GTAACCCTCG GCGGCAGCAT CGCCGATCCG CACTTGGGCG GCAGCATCAA 2901 CGGCGACAAA CTCTATTACC GCAACCAAAC CCAAGGCATC ATCTTGGACA 2951 ACGGCTCGCT GCGTTCGCAT ATCGCGGGCA GGAAATGGGT AATCGACAGC 3001 CTGAAATTCC GGCACGAAGG GACGGCGGAA CTCTCCGGTA CGGTCGGTAT 3051 GGAAAACAGC GGACCCGATG TCGATATCGG CGCGGTGTTC GACAAATACC 3101 GCATCCTGTC CCGCCCCAAC CGCCGCCTGA CGGTTTCCGG CAACACCCGC 3151 CTGCGCTATT CGCCGCAAAA AGGCATATCC GTTACCGGGA TGATTAAAAC 3201 GGATCAGGGG CTGTTCGGTT CGCAAAAATC CTCGATGCCG TCCGTCGGCG 3251 ACGATGTCGT CGTATTAGGC GAAGTCAAAA AAGAGGCGGC GGCACCGCTC 3301 CCCGTCAATA TGAACCTGAC TTTAGACCTC AATGACGGCA TCCGCTTCGC 3351 CGGCTACGGC GCGGACGTTA CCATAGGCGG CAAACTGACC CTGACCGCCC 3401 AATCGGGCGG AAGCGTACGG GGCGTGGGCA CGGTCCGCGT CATCAAAGGG 3451 CGTTATAAGG CATACGGGCA GGATTTGGAC ATTACCAAAG GCACGGTCTC 3501 CTTTGTCGGC CCGCTCAACG ATCCCAACCT CAACATCCGC GCCGAACGCC 3551 GCCTTTCCCC CGTCGGTGCG GGCGTGGAAA TATTGGGCAG CCTCAACAGC 3601 CCGCGCATTA CGCTGACGGC AAACGAACCG ATGAGTGAAA AAGACAAGCT 3651 CTCTTGGCTC ATCCTCAACC GCGCCGGCAG CGGCAGCAGC GGCGACAATG 3701 CCGCCCTGTC TGCAGCCGCA GGTGCGCTGC TTGCCGGGCA AATCAACGAC 3751 CGCATCGGGC TGGTGGATGA TTTGGGCTTT ACCAGCAAGC GCAGCCGCAA 3801 CGCGCAAACC GGCGAACTCA ACCCCGCCGA ACAGGTGCTG ACCGTCGGCA 3851 AACAACTGAC CGGCAAACTC TACATCGGCT ACGAATACAG CATCTCCAGC 3901 GCGGAACAGT CCGTCAAACT GATTTACCGG CTGACCCGCG CCATACAGGC 3951 GGTTGCCCGT ATCGGCAGCC GTTCGTCGGG CGGCGAGCTG ACATACACCA 4001 TACGTTTCGA CCGCTTCTCC GGTTCGGACA AAAAAGACTC CGCCGGAAAC 4051 GGCAAAGGAA AATAA This corresponds to the amino acid sequence <SEQ ID 1190; ORF 285- 1>: m285-l.pep 1 LKLSAALLSV LILAVCFLGW LAGTEAGLRF GLYQIPSWFG VNISSQNLKG 51 TLLDGFDGDN WSIETEGADL KISRFRFAWK PSELMRRSLH ITEISAGDIA 101 IVTKPTPPKE ERPPLSLPDΞ IDLPAAVYLD RFETGKISMG KAFDKQTVYL 151 ERLDASYRYD RKGHRLDLKA ADTPWΞΞSSG AASVGLKKPF ALDTAIYTKG 201 GLEGKTIHST ARLΞGΞLKDV RAELAIDGGN IRLSGKSVIH PFAESLDKTL 251 EEVLVKGFNI NPAAFVPSLP DAGLNFDLTA IPSFSDGIAL EGSLDLENTK 301 AGFADRNGIP VRQVLGGFVI RQDGTVHIGN TSAALLGRGG IRLSGKIDTE 351 KDILDLNIGI NSVGAEDVLQ TAFKGRLDGS IGIGGTTASP KIΞWQLGIGT 401 ARTDGSLAIA SDPANGQRKL VLDTVNIAAG QGΞLTAQGYL ELFKDRLLKL 451 DIRSRAFDPS RIDPQLPAGN INGSINLAGE LAKEKFTGKM RFLPGTFNGV 501 PIAGSADIVY ESRHLPRAAV DLRLGRNIIK TDGGFGKKGD RLNLNITAPD 551 LSRFGFGLAG SLNVRGHLSG DLDGGIRTFE TDLΞGAARNL HIGKAADIRS 601 LDFTLKGΞPD TΞRPIRADIK GSRLSLSGGA AVVDTADLML DGTGVQHRIR 651 THAAMTLDGK PFKFDLDASG GINRELTRWK GSIGILDIGG AFNLKLQNRM 701 TLEAGAERVA ASAANWQAMG GSLNLQHFSW DKKTGISAKG GAHGLHIAEL 751 HNFFKPPFEH NLVLNGDWDV AYGRNARGYL NISRQSGDAV LPGGQALGLN 801 AFSLKTRFQN DRIGILLDGG ARFGRINADL GIANAFGGNM ANAPLGGRIT 851 ASLPDLGALK PFLPAAAQNI TGSLNAAAQI GGRVGSPSVN AAVNGSΞNYG 901 KINGNITVGQ SRSFDTAPLG GRLNLTVADA EVFRNFLPVG QTVKGSLNAA 951 VTLGGSIADP HLGGSINGDK LYYRNQTQGI ILDNGSLRSH IAGRKWVIDS 1001 LKFRHEGTAE LSGTVGMENS GPDVDIGAVF DKYRILSRPN RRLTVSGNTR 1051 LRYSPQKGIS VTGMIKTDQG LFGSQKSSMP SVGDDVVVLG EVKKEAAAPL 1101 PVNMNLTLDL NDGIRFAGYG ADVTIGGKLT LTAQSGGSVR GVGTVRVIKG 1151 RYKAYGQDLD ITKGTVSFVG PLNDPNLNIR AERRLSPVGA GVEILGSLNS 1201 PRITLTANEP MSEKDKLSWL ILNRAGSGSS GDNAALSAAA GALLAGQIND 1251 RIGLVDDLGF TSKRSRNAQT GELNPAEQVL TVGKQLTGKL YIGYEYSISS 1301 AEQSVKLIYR LTRAIQAVAR IGSRSSGGEL TYTIRFDRFS GSDKKDSAGN 1351 GKGK* g285-l/m285-l 96.5% identity in 1354 aa overlap 10 20 30 40 50 60 g285-1. pep LKLSAALLSVLILAVCFLGWIAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDN I I II I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m285-l LKLSAALLSVLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDN 10 20 30 40 50 60 70 80 90 100 110 120 g285-1. pep WSIETEGADLKIΞRFRFAWKPSELMRRSLHITDISAGDIAIVTKPTPPKEERPPQGLPDS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I h I I I I I I I I I I I I I I I I I I I I I :| I I I m285-l WSIETEGADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDS 70 80 90 100 110 120 130 140 150 160 170 180 g285-1. pep IDLPAAVYLDRFETGKISMGKTFDKQTVYLERLNAAYRYDRKGHRLDLKAADTPWSSSSG I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I : I : I I I I I I I I I I I I I I I I I I I I I I I I m285-l IDLPAAVYLDRFETGKISMGKAFDKQTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSG 130 140 150 160 170 180 190 200 210 220 230 240 g285-1. pep SASVGLKKPFALDTAIYTKGGFEGETIHSTARLSGSLKDVRAELTIDGGNIRLSGKSVIH : I I I I I II I I I I I I I I I I I I hi I : I I I I I I I I I I I I I I I I I I hi I I I I I I I I I I I I II m285-l AASVGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIH 190 200 210 220 230 240 250 260 270 280 290 300 g285-l.pep PFAESLDKTLEEVLVKGFNINPSAFVPSLPDAGLNFDLTAIPΞFSDGIALEGSLDLENTK I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m285-1 PFAESLDKTLEEVLVKGFNINPAAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENTK 250 260 270 280 290 300 310 320 330 340 350 360 g285-l.pep AGFADRNGIPVRQVLGGFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLNIGI I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m285-l AGFADRNGIPVRQVLGGFVIRQDGTVHIGNTΞAALLGRGGIRLSGKIDTEKDILDLNIGI 310 320 330 340 350 360 370 380 390 400 410 420 g285-1. pep NSVGAEDVLQTAFKGRLDGΞIGIGGTTASPKISWQLGTGTARTDGΞLAIAΞDPANEQRKL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I ll lllllll llll m285-l NSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPANGQRKL 370 380 390 400 410 420 430 440 450 460 470 480 g285-l.pep VFDTVNIΞAGEGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQFPAGDINGSIHLAGE I : I I I I h I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I :l I I I I : I I I I m285-l VLDTVNIAAGQGΞLTAQGYLELFKDRLLKLDIRΞRAFDPSRIDPQLPAGNINGSINLAGE 430 440 450 460 470 480 490 500 510 520 530 540 g285-1. pep LAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIVKTDGGFGKKGD I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I :| I I I I I I I I I I m285-l LAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKTDGGFGKKGD 490 500 510 520 530 540 550 560 570 580 590 600 g285-l.pep RLNLNITAPDLSRFGFGLAGΞLNVRGHLSGDLDGGIRTFETDLSGTARNLHIGKAADIRS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I: I I I I II I I I I I I I I m285-l RLNLNITAPDLSRFGFGLAGSLNVRGHLΞGDLDGGIRTFETDLSGAARNLHIGKAADIRS 550 560 570 580 590 600 610 620 630 640 650 660 g285-l.pep LDFTLKGSPGTSRPMRADIKGGRLSLSGGAAVVDTAGLTLEGTGAQHRIRTHAAMTLDGK lllllllll I I I I :l I I I I I : I I I I I I I I I I I I I I I I : I I I : I I I I I I I I I I I I I I I m285-l LDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQHRIRTHAAMTLDGK 610 620 630 640 650 660 670 680 690 700 710 720 g285-1. pep PFKLDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAGAEHVAASAANWQAMG I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I m285-l PFKFDLDASGGINRELTRWKGΞIGILDIGGAFNLKLQNRMTLEAGAERVAASAANWQAMG 670 680 690 700 710 720 730 740 750 760 770 780 g285-1. pep GSLNLQHFSWDRKTGISAKGGARGLHIAELHNFFKPPFEHNLVLNGDWDVAYGHNARGYL I I I I I I I I I I hi I I I I I I I I I :| I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I m285-l GSLNLQHFSWDKKTGISAKGGAHGLHIAELHNFFKPPFEHNLVLNGDWDVAYGRNARGYL 730 740 750 760 770 780 790 800 810 820 830 840 g285-1. pep NISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGARFGRINADLGIGNAFGGNM I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I hi I I I I I I m285-l NISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGARFGRINADLGIANAFGGNM 790 800 810 820 830 840 850 860 870 880 890 900 g285-1. pep ANTPLGGRITASLPDLGALKPFLPAAAQNITGSLNASAQIGGRVGSPSVNAAVNGSSNYG I h I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I m285-l ANAPLGGRITASLPDLGALKPFLPAAAQNITGSLNAAAQIGGRVGSPSVNAAVNGSSNYG 850 860 870 880 890 900 910 920 930 940 950 960 g285-1. pep KINGNITVGQSRΞFDTAPLGGRLNLTVADAEAFRNFLPVGQTVKGSLNAAVTLGGSIADP I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I m285-l KINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFLPVGQTVKGSLNAAVTLGGSIADP 910 920 930 940 950 960 970 980 990 1000 1010 1020 g285-1. pep HLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRKWVIDSLKFRHEGTAELSGTVSMENS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I h I II I m285-l HLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRKWVIDSLKFRHEGTAELSGTVGMENS 970 980 990 1000 1010 1020 1030 1040 1050 1060 1070 1080 g285-1. pep VPDVDIGAVFDKYRILΞRPNRRLTVSGNTRLRYSPQKGIΞVTGMIKTDQGLFGSQKSSMP I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m285-l GPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRYSPQKGISVTGMIKTDQGLFGSQKSSMP 1030 1040 1050 1060 1070 1080 1090 1100 1110 1120 1130 1140 g285-1.pep SVGDDVVVLGEVKKEAAASLPVNMNLTLDLNDGIRFSGYGADVTIGGKLTLTAQPGGNVR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I: I I m285-l SVGDDVVVLGEVKKEAAAPLPVNMNLTLDLNDGIRFAGYGADVTIGGKLTLTAQSGGSVR 1090 1100 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 g285-1.pep GVGTVRVIKGRYKAYGQDLDITKGTVSFVGPLNDPNLNIRAERRLΞPVGAGVEILGΞLNS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I m285-l GVGTVRVIKGRYKAYGQDLDITKGTVSFVGPLNDPNLNIRAERRLSPVGAGVEILGΞLNS 1150 1160 1170 1180 1190 1200 1210 1220 1230 1240 1250 1260 g285-1. pep PRITLTANEPMSEKDKLSWLILNRAGSGSΞGDNAALSAAAGALLAGQINDRIGLVDDLGF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m285-1 PRITLTANEPMSEKDKLSWLILNRAGSGSSGDNAALSAAAGALLAGQINDRIGLVDDLGF 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 1310 1320 g285-l.pep TSKRSRNAQTGELNPAEQVLTVGKQLTGKLYIGYEYGISSAEQSVKLIYRLTRAIQAVAR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I hi I I I I I I I I I I I I I I I I I I I I I I m285-l TSKRSRNAQTGELNPAEQVLTVGKQLTGKLYIGYEYΞIΞSAEQΞVKLIYRLTRAIQAVAR 1270 1280 1290 1300 1310 1320 1330 1340 1350 g285-l .pep IGSRSSGGELTYTIRFDRLFGΞDKKDΞAGNGKGK I I I I I I I I I I I I I I I I I h llllllllllllll m285-l IGSRSSGGELTYTIRFDRFSGSDKKDSAGNGKGKX 1330 1340 1350 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 1191>: a285-l.seq 1 CTGAAGCTGT CGGCGGCACT GCTGTCTGTT CTGATTTTGG CAGTATGTTT 51 CCTCGGCTGG CTCGCCGGCA CGGAAGCGGG TTTGCGCTTC GGGCTGTACC 101 AAATCCCGTC TTGGTTCGGC GTAAACATTT CCTCCCAAAA CCTCAAAGGC 151 ACGCTGCTCG ACGGCTTCGA CGGCGACAAC TGGTCGATAG AAACCGAGGG 201 GGCAGACCTT AAAATCAGCC GCTTCCGCTT CGCGTGGAAA CCGTCCGAAC 251 TGATGCGCCG CAGCCTGCAC ATTACCGAAA TTTCCGCCGG CGACATCGCC 301 ATCGTTACCA AACCGACTCC GCCTAAAGAA GAACGCCCGC CGCTCAGCCT 351 TCCCGACAGC ATAGACCTGC CTGCCGCCGT CTATCTCGAC CGCTTCGAGA 401 CGGGCAAAAT CAGCATGGGC AAAGCCTTTG ACAAACAAAC CGTCTATCTC 451 GAACGGCTGG ATGCTTCATA CCGTTACGAC CGCAAAGGAC ACCGCCTCGA 501 CCTGAAGGCT GCCGACACGC CGTGGAGCAG TTCGTCGGGG TCAGCCTCGG 551 TCGGCTTGAA AAAACCGTTT GCCCTCGATA CCGCCATTTA CACCAAAGGC 601 GGACTCGAAG GCAAAACCAT ACACAGTACG GCTCGGCTGA GCGGCAGCCT 651 GAAGGATGTG CGCGCCGAAC TGGCGATCGA CGGCGGCAAT ATCCGCCTCT 701 CGGGAAAATC CGTCATCCAC CCGTTTGCCG AATCATTGGA TAAAACATTG 751 GAAGAAGTAC TGGTCAAAGG GTTCAACATC AATCCGTCCG CCTTCGTGCC 801 TTCCCTGCCC GATGCCGGGC TGAATTTCGA CCTGACCGCC ATCCCGTCGT 851 TTTCAGACGG CATCGCGCTG GAAGGCTCGC TCGATTTGGA AAACACCAAA 901 GCCGGCTTTG CCGACCGCAA CGGCATCCCC GTCCGTCAGG TTTTAGGCAG 951 CTTTGTCATC CGGCAGGACG GCACGGTGCA TATCGGCAAT ACGTCCGTCG 1001 CCCTGCTCGG ACGGGGCGGC ATCAGGCTGT CGGGCAAAAT CGACACCGAA 1051 AAAGACATCC TCGATTTAAA TATAGGCATC AACTCCGTCG GCGCGGAAGA 1101 CGTACTGCAA ACCGCGTTCA AAGGCAGGTT GGACGGCAGC ATCGGCATCG 1151 GTGGCACGAC CGCCTCGCCC AAAATCTCTT GGCAACTCGG CATCGGCACG 1201 GCGCGCACGG ACGGCAGCCT CGCCATTGCA AGCGACCCCG CAAACGGACA 1251 GCGGAAACTG GTGCTCGACA CCGTCAACAT CGCCGCCGGG CAAGGCAGCC 1301 TGACCGCGCA AGGCTATCTC GAGCTGTTTA AAGACCGCCT GCTCAAGCTG 1351 GACATCCGTT CCCGCGCATT CGACCCTTCG CGCATCGATC CGCAACTTCC 1401 GGCAGGCAAT ATCAACGGCT CAATAAACCT TGCCGGCGAA CTGGCAAAAG 1451 AGAAATTCAC AGGCAAAATG CGGTTTTTAC CCGGCACGTT CAACGGCGTA 1501 CCGATTGCCG GCAGTGCCGA CATTGTTTAC GAGTCCCGCC ACCTTCCGCG 1551 TGCCGCCGTC GATTTGCGGC TGGGGCGGAA CATTATTAAA ACAGACGGCG 1601 GCTTCGGCAA AAAAGGCGAC CGGCTTAACC TCAATATCAC CGCACCCGAT 1651 TTATCCCGTT TCGGTTTCGG ACTCGCGGGG TCTTTAAATG TACGCGGACA 1701 CCTTTCCGGC GATTTGGACG GTGGCATCCG AACCTTTGAA ACCGACCTTT 1751 CCGGCGCGGC GCGCAACCTG CACATCGGCA AGGCGGCAGA CATCCGTTCG 1801 CTCGATTTCA CGCTCAAAGG TTCGCCCGAC ACAAGCCGCC CGATACGCGC 1851 CGACATCAAA GGCAGCCGCC TTTCGCTGTC GGGCGGAGCG GAGGTTGTCG 1901 ATACCGCCGA CCTGATGCTG GACGGCACGG GCGTGCAGCA CCGCATCCGC 1951 ACACACGCCG CCATGACGCT GGATGGCAAA CCGTTCAAAT TCGATTTGGA 2001 CGCTTCAGGC GGCATCAACA GGGAACTTAC CCGATGGAAA GGCAGCATCG 2051 GCATCCTCGA CATCGGCGGC GCATTCAACC TCAAGCTGCA AAACCGTATG 2101 ACGCTCGAAG CCGGTGCGGA ACGCGTGGCG GCAAGTGCGG CAAATTGGCA 2151 GGCAATGGGC GGCAGCCTCA ACCTGCAACA CTTTTCTTGG GATAAAAAAA 2201 CCGGCATATC GGCAAAAGGC GGCGCACACG GTCTGCATAT CGCCGAGTTG 2251 CACAATTTCT TCAAACCGCC CTTCGAACAC AATCTGGTTT TAAACGGCGA 2301 CTGGGATGTC GCCTACGGGC GAAACGCGCG CGGCTACCTC AATATCAGCC 2351 GGCAAAGCGG CGATGCCGTA TTGCCCGGCG GGCAGGCTTT GGGTTTGAAC 2401 GCATTTTCCC TGAAAACGCG CTTTCAAAAC GACCGTATCG GAATCCTGCT 2451 TGACGGCGGC GCGCGTTTCG GGCGGATTAA CGCCGATTTG GACATCGGCA 2501 ACGCCTTCGG CGGCAATATG GCAAATGCAC CGCTCGGCGG CAGGATTACC 2551 GCCTCCCTTC CCGACTTGGG CACATTGAAG CCCTTTCTGC CCGCCGCCGC 2601 GCAAAACATT ACCGGCAGCC TGAATGCCGC CGCGCAAATC GGCGGACGGG 2651 TCGGCTCTCC GTCCGTCAAT GCCGCCGTCA ACGGCAGCAG CAACTACGGG 2701 AAAATCAACG GCAACATCAC CGTCGGGCAA AGCCGCTCTT TCGATACCGC 2751 GCCTTTGGGC GGCAGGCTCA ACCTGACCGT TGCCGATGCC GAAGTATTCC 2801 GCAACTTCCT ACCGGTCGGA CAAACCGTCA AAGGCAGCCT GAATGCCGCC 2851 GTAACCCTCG GCGGCAGCAT CGCCGATCCG CACTTGGGCG GCAGCATCAA 2901 CGGCGACAAA CTCTATTACC GCAACCAAAC CCAAGGCATC ATCTTGGACA 2951 ACGGCTCGCT GCGTTCGCAT ATCGCGGGCA GGAAATGGGT AATCGACAGC 3001 CTGAAATTCC GGCACGAAGG GACGGCGGAA CTCTCCGGTA CGGTCGGTAT 3051 GGAAAACAGC GGACCCGATG TCGATATCGG CGCGGTGTTC GACAAATACC 3101 GCATCCTGTC CCGCCCCAAC CGCCGCCTGA CGGTTTCCGG CAACACCCGC 3151 CTGCGCTATT CGCCGCAAAA AGGCATATCC GTTACCGGGA TGATTAAAAC 3201 GGATCAGGGG CTGTTCGGTT CGCAAAAATC CTCGATGCCG TCCGTCGGCG 3251 ACGATGTCGT CGTATTAGGC GAAGTCAAAA AAGAGGCGGC GGCACCGCTC 3301 CCCGTCAATA TGAACCTGAC TTTAGACCTC AATGACGGCA TCCGCTTCGC 3351 CGGCTACGGC GCGGACGTTA CCATAGGCGG CAAACTGACC CTGACCGCCC 3401 AATCGGGCGG AAGCGTGCGG GGCGTGGGCA CGGTCCGCGT CATCAAAGGG 3451 CGTTATAAGG CATACGGGCA GGATTTGGAC ATTACCAAAG GCACGGTCTC 3501 CTTTGTCGGC CCGCTCAACG ACCCCAACCT CAACATCCGC GCCGAACGCC 3551 GCCTTTCCCC CGTCGGTGCG GGCGTGGAAA TATTGGGCAG CCTCAACAGT 3601 CCGCGCATTA CGCTGACGGC AAACGAACCG ATGAGTGAAA AAGACAAGCT 3651 CTCCTGGCTC ATCCTCAACC GCGCCGGCAG TGGCAGCAGC GGCGACAATG 3701 CCGCCCTGTC CGCAGCCGCC GGCGCGCTGC TTGCCGGGCA AATCAACGAC 3751 CGCATCGGGC TGGTGGATGA TTTGGGCTTT ACCAGCAAGC GCAGCCGCAA 3801 CGCGCAAACC GGCGAACTCA ACCCCGCCGA ACAGGTGCTG ACCGTCGGCA 3851 AACAACTGAC CGGCAAACTC TACATCGGCT ACGAATACAG CATCTCCAGC 3901 GCGGAACAGT CCGTCAAACT GATTTACCGG CTGACCCGCG CCATACAGGC 3951 GGTTGCCCGT ATCGGCAGCC GTTCGTCGGG CGGCGAGCTG ACATACACCA 4001 TACGTTTCGA CCGCTTCTCC GGTTCGGACA AAAAAGACTC CGCCGGAAAC 4051 AGCAAAGGAA AATAA This corresponds to the amino acid sequence <SEQ ID 1192; ORF 285-1. a>: a285-l.pep 1 LKLSAALLSV LILAVCFLGW LAGTEAGLRF GLYQIPSWFG VNISSQNLKG 51 TLLDGFDGDN WSIETEGADL KISRFRFAWK PSELMRRSLH ITEISAGDIA 101 IVTKPTPPKE ERPPLSLPDS IDLPAAVYLD RFETGKISMG KAFDKQTVYL 151 ERLDASYRYD RKGHRLDLKA ADTPWSSSSG SASVGLKKPF ALDTAIYTKG 201 GLEGKTIHST ARLSGSLKDV RAELAIDGGN IRLSGKSVIH PFAESLDKTL 251 EEVLVKGFNI NPSAFVPSLP DAGLNFDLTA IPSFSDGIAL EGSLDLENTK 301 AGFADRNGIP VRQVLGSFVI RQDGTVHIGN TSVALLGRGG IRLSGKIDTE 351 KDILDLNIGI NSVGAEDVLQ TAFKGRLDGS IGIGGTTASP KISWQLGIGT 401 ARTDGSLAIA SDPANGQRKL VLDTVNIAAG QGSLTAQGYL ELFKDRLLKL 451 DIRSRAFDPS RIDPQLPAGN INGSINLAGE LAKEKFTGKM RFLPGTFNGV 501 PIAGSADIVY ESRHLPPAAV DLRLGRNIIK TDGGFGKKGD RLNLNITAPD 551 LSRFGFGLAG SLNVRGHLSG DLDGGIRTFE TDLSGAARNL HIGKAADIRS 601 LDFTLKGSPD TSRPIRADIK GSRLSLSGGA EVVDTADLML DGTGVQHRIR 651 THAAMTLDGK PFKFDLDASG GINRELTRWK GSIGILDIGG AFNLKLQNRM 701 TLEAGAERVA ASAANWQAMG GSLNLQHFSW DKKTGISAKG GAHGLHIAEL 751 HNFFKPPFEH NLVLNGDWDV AYGRNARGYL NISRQSGDAV LPGGQALGLN 801 AFSLKTRFQN DRIGILLDGG ARFGRINADL DIGNAFGGNM ANAPLGGRIT 851 AΞLPDLGTLK PFLPAAAQNI TGSLNAAAQI GGRVGSPSVN AAVNGΞSNYG 901 KINGNITVGQ SRSFDTAPLG GRLNLTVADA EVFRNFLPVG QTVKGSLNAA 951 VTLGGSIADP HLGGSINGDK LYYRNQTQGI ILDNGSLRSH IAGRKWVIDS 1001 LKFRHEGTAE LSGTVGMENS GPDVDIGAVF DKYRILSRPN RRLTVSGNTR 1051 LRYSPQKGIS VTGMIKTDQG LFGSQKSSMP SVGDDVVVLG EVKKEAAAPL 1101 PVNMNLTLDL NDGIRFAGYG ADVTIGGKLT LTAQSGGSVR GVGTVRVIKG 1151 RYKAYGQDLD ITKGTVSFVG PLNDPNLNIR AERRLSPVGA GVEILGSLNS 1201 PRITLTANEP MSEKDKLSWL ILNRAGSGSS GDNAALSAAA GALLAGQIND 1251 RIGLVDDLGF TSKRSRNAQT GELNPAEQVL TVGKQLTGKL YIGYEYSISΞ 1301 AEQSVKLIYR LTRAIQAVAR IGSRSSGGEL TYTIRFDRFS GΞDKKDΞAGN 1351 SKGK* a285-l/m285-l 99.3% identity in 1354 aa overlap 10 20 30 40 50 60 a285-1. pep LKLSAALLSVLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m285-l LKLΞAALLΞVLILAVCFLGWLAGTEAGLRFGLYQIPΞWFGVNIΞSQNLKGTLLDGFDGDN 10 20 30 40 50 60 70 80 90 100 110 120 a285-1. pep WΞIETEGADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDΞ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I m285-l SIETEGADLKISRFRFAWKPΞELMRRΞLHITEISAGDIAIVTKPTPPKEERPPLΞLPDΞ 70 80 90 100 110 120 130 140 150 160 170 180 a285-l.pep IDLPAAVYLDRFETGKI8MGKAFDKQTVYLERLDAΞYRYDRKGHRLDLKAADTPWSSSΞG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m285-l IDLPAAVYLDRFETGKI8MGKAFDKQTVYLERLDAΞYRYDRKGHRLDLKAADTPWSSSSG 130 140 150 160 170 180 190 200 210 220 230 240 a285-l.pep 8ASVGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIH : I I I I I I I I I I I I I I I I II I I I I II I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I m285-l AASVGLKKPFALDTAIYTKGGLEGKTIHSTARLSGΞLKDVRAELAIDGGNIRLSGKΞVIH 190 200 210 220 230 240 250 260 270 280 290 300 a285-1. pep PFAESLDKTLEEVLVKGFNINPSAFVPΞLPDAGLNFDLTAIPΞFSDGIALEGΞLDLENTK I I I I I I I I I I I I I I I I I I I I I hi I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m285-l PFAESLDKTLEEVLVKGFNINPAAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENTK 250 260 270 280 290 300 310 320 330 340 350 360 a285-l.pep AGFADRNGIPVRQVLGΞFVIRQDGTVHIGNTSVALLGRGGIRLΞGKIDTEKDILDLNIGI I I I I I I I I I I I I I I I I :l I I I I I I I I II I I I I = 1 I I I I I I I I I I I I I I I I I I I I I I I I I I m285-l AGFADRNGIPVRQVLGGFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLNIGI 310 320 330 340 350 360 370 380 390 400 410 420 a285-l.pep NSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIAΞDPANGQRKL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m285-1 NSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIAΞDPANGQRKL 370 380 390 400 410 420 430 440 350 460 470 480 a285-1. pep VLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQLPAGNINGSINLAGE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m285-l VLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQLPAGNINGSINLAGE 430 440 450 460 470 480 490 500 510 520 530 540 a285-1. pep LAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKTDGGFGKKGD I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m285-1 LAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKTDGGFGKKGD 490 500 510 520 530 540 550 560 570 580 590 600 a285-1. pep RLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLΞGAARNLHIGKAADIRS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m285-l RLNLNITAPDLSRFGFGLAGSLNVRGHLΞGDLDGGIRTFETDLSGAARNLHIGKAADIRS 550 560 570 580 590 600 610 620 630 640 650 660 a285-l.pep LDFTLKGSPDTΞRPIRADIKGSRLSLSGGAEVVDTADLMLDGTGVQHRIRTHAAMTLDGK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m285-l LDFTLKGSPDTSRPIRADIKGSRLΞLΞGGAAVVDTADLMLDGTGVQHRIRTHAAMTLDGK 610 620 630 640 650 660 670 680 690 700 710 720 a285-1. pep PFKFDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAGAERVAASAANWQAMG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m285-1 PFKFDLDASGGINRELTRWKG IGILDIGGAFNLKLQNRMTLEAGAERVAASAANWQAMG 670 680 690 700 710 720 730 740 750 760 770 780 a285-l.pep GΞLNLQHFSWDKKTGIΞAKGGAHGLHIAELHNFFKPPFEHNLVLNGDWDVAYGRNARGYL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m285-1 GSLNLQHFSWDKKTGIΞAKGGAHGLHIAELHNFFKPPFEHNLVLNGDWDVAYGRNARGYL 730 740 750 760 770 780 790 800 810 820 830 840 a285-l.pep NISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGARFGRINADLDIGNAFGGNM I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I h I I I I I I I m285-l NISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGARFGRINADLGIANAFGGNM 790 800 810 820 830 840 850 860 870 880 890 900 a285-l.pep ANAPLGGRITAΞLPDLGTLKPFLPAAAQNITGSLNAAAQIGGRVGΞPSVNAAVNGSSNYG I I I I I I I I I I I I I I I I hi I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m285-1 ANAPLGGRITASLPDLGALKPFLPAAAQNITGSLNAAAQIGGRVGΞPSVNAAVNGSSNYG 850 860 870 880 890 900 910 920 930 940 950 960 a285-l.pep KINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFLPVGQTVKGSLNAAVTLGGΞIADP I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m285-l KINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFLPVGQTVKGSLNAAVTLGGΞIADP 910 920 930 940 950 960 970 980 990 1000 1010 1020 a285-1. pep HLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRKWVIDSLKFRHEGTAELSGTVGMENS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m285-l HLGGSINGDKLYYRNQTQGIILDNGΞLRSHIAGRKWVIDSLKFRHEGTAELΞGTVGMENS 970 980 990 1000 1010 1020 1030 1040 1050 1060 1070 1080 a285-1. pep GPDVDIGAVFDKYRILΞRPNRRLTVΞGNTRLRYSPQKGISVTGMIKTDQGLFGΞQKΞSMP I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m285-l GPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRYSPQKGIΞVTGMIKTDQGLFGΞQKSSMP 1030 1040 1050 1060 1070 1080 1090 1100 1110 1120 1130 1140 a285-1. pep SVGDDVVVLGEVKKEAAAPLPVNMNLTLDLNDGIRFAGYGADVTIGGKLTLTAQSGGSVR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m285-l SVGDDVVVLGEVKKEAAAPLPVNMNLTLDLNDGIRFAGYGADVTIGGKLTLTAQSGGΞVR 1090 1100 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 a285-l.pep GVGTVRVIKGRYKAYGQDLDITKGTVSFVGPLNDPNLNIRAERRLSPVGAGVEILGΞLNS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m285-l GVGTVRVIKGRYKAYGQDLDITKGTVSFVGPLNDPNLNIRAERRLSPVGAGVEILGSLNS 1150 1160 1170 1180 1190 1200 1210 1220 1230 1240 1250 1260 a285-1. pep PRITLTANEPMSEKDKLSWLILNRAGSGSΞGDNAALΞAAAGALLAGQINDRIGLVDDLGF I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m285-l PRITLTANEPMΞEKDKLΞWLILNRAGSGSSGDNAALSAAAGALLAGQINDRIGLVDDLGF 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 1310 1320 a285-l.pep TSKRSRNAQTGELNPAEQVLTVGKQLTGKLYIGYEYSIΞΞAEQSVKLIYRLTRAIQAVAR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m285-l TΞKRSRNAQTGELNPAEQVLTVGKQLTGKLYIGYEYSISΞAEQSVKLIYRLTRAIQAVAR 1270 1280 1290 1300 1310 1320 1330 1340 1350 a285-l .pep IGSRSSGGELTYTIRFDRFSGΞDKKDΞAGNSKGKX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I hi I I I m285-l IGΞRΞΞGGELTYTIRFDRFSGSDKKDSAGNGKGKX 1330 1340 1350 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1193>: g286. seq 1 atgcagaaca ccggtaccat gatgatcaaa ccgaccgccc tgctcctgcc 51 ggctttattt ttctttccgc acgcatacgc gcctgccgcc gacctttccg 101 aaaacaaggc ggcgggtttc gcattgttca aaagcaaaag ccccgacacc 151 gaatcagtca aattaaaacc caaattcccc gtccgcatcg acacgcagga 201 cagtgaaatc aaagatatgg tcgaagaaca cctgccgctc atcacgcagc 251 agcaggaaga ggttttggat aaggaacaga cgggattcct tgccgaagaa 301 gcaccggaca acgttaaaac aatgctccgc agcaaaggct atttcagcag 351 caaggtcagc ctgacggaaa aagacggagc ttatacggtg cacatcacac 401 cgggcccgcg caccaaaatc gccaacgtcg gcgtcgccat cctcggcgac 451 atcctttcag acggcaacct cgccgaatac taccgcaacg cgctggaaaa 501 ctggcagcag ccggtaggca gcgatttcga tcaggacagt tgggaaaaca 551 gcaaaacttc cgtcctcggc gcggtaacgc gcaaaggcta cccgcttgcc 601 aagctcggca acacccgggc ggccgtcaac cccgataccg ccaccgccga 651 tttgaacgtc gtcgtggaca gcggccgccc cattgccttc ggcgactttg 701 aaatcaccgg cacacagcgt taccccgaac aaaccgtctc cggcctggcg 751 cgcttccaac cgggcacgcc ctacgacctc gacctgctgc tcgacttcca 801 acaggcgctc gaacaaaacg ggcattattc cggcgcgtcc gtacaagccg 851 acttcgaccg cctcccaagg ggaccgcgtc cccgtcaaag tcagcgtaac 901 cgaggtcaaa cgccacaaac tcgaaaccgg catccgcctc gattcggaat 951 acggtttggg cggcaaaatc gcctacgact attacaacct cttcaacaaa 1001 ggctatatcg gctcggtcgt ctgggatatg gacaaatacg aaaccacgct 1051 tgccgccggc atcagccagc cgcgcaacta tcggggcaac tactggacaa 1101 gcaacgtttc ctacaaccgt tcgaccaccc aaaacctcga aaaacgcgcc 1151 ttctccggcg gcatctggta tgtgcgcgac cgcgcgggca tcgatgccag 1201 gctgggggcg gaatttctcg cagaaggccg gaaaatcccc ggctcggatg 1251 tcgatttggg caacagccac gccacgatgc tgaccgcctc ttggaaacgc 1301 cagctgctca acaacgtgct gcaccccgaa aacggccatt acctcgacgg 1351 caaaatcggg acgactttgg gcacattcct gtcctccacc gcgctaatcc 1401 gcacctctgc ccgcgcaggt tatttcttca cgcccgaaaa caaaaaactc 1451 ggcacgttca tcatacgcgg acaagcgggt tacaccgttg cacgcgacaa 1501 tgccgatgtc ccctcggggc tgatgttccg cagcggcggc gcgtcttccg 1551 tgcgcggtta cgaacttga This corresponds to the amino acid sequence <SEQ ID 1194; ORF 286.ng>: g286.pep 1 MQΝTGTMMIK PTALLLPALF FFPHAYAPAA DLSEΝKAAGF ALFKSKSPDT 51 ESVKLKPKFP VRIDTQDSEI KDMVEEHLPL ITQQQEEVLD KEQTGFLAEE 101 APDΝVKTMLR SKGYFSSKVS LTEKDGAYTV HITPGPRTKI AΝVGVAILGD 151 ILSDGΝLAEY YRΝALEΝWQQ PVGSDFDQDS WEΝSKTSVLG AVTRKGYPLA 201 KLGΝTRAAVΝ PDTATADLΝV WDSGRPIAF GDFEITGTQR YPEQTVSGLA 251 RFQPGTPYDL DLLLDFQQAL EQΝGHYSGAS VQADFDRLPR GPRPRQSQRΝ 301 RGQTPQTRΝR HPPRFGIRFG RQΝRLRLLQP LQQRLYRLGR LGYGQIRΝHA 351 CRRHQPAAQL SGQLLDKQRF LQPFDHPKPR KTRLLRRHLV CARPRGHRCQ 401 AGGGISRRRP EΝPRLGCRFG QQPRHDADRL LETPAAQQRA APRKRPLPRR 451 QNRDDFGHIP VLHRANPHLC PRRLFLHARK QKTRHVHHTR TSGLHRCTRQ 501 CRCPLGADVP QRRRVFRARL RT* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1195>: m2i 6. seq 1 ATGCACGACA CCCGTACCAT GATGATCAAA CCGACCGCCC TGCTCCTGCC 51 GGCTTTATTT TTCTTTCCGC ACGCATACGC GCCTGCCGCC GACCTTTCCG 101 AAAACAAGGC GGCGGGTTTC GCATTGTTCA AAAACAAAAG CCCCGACACC 151 GAATCAGTCA AATTAAAACC CAAATTCCCC GTCCTCATCG ACACGCAGGA 201 CAGTGAAATC AAAGATATGG TCGAAGAACA CCTGCCGCTC ATCACGCAGC 251 AGCAGGAAGA AGTATTGGAC AAGGAACAGA CGGGCTTCCT CGCCGAAGAA 301 GCGCCGGACA ACGTTAAAAC GATGCTCCGC AGCAAAGGCT ATTTCAGCAG 351 CAAAGTCAGC CTGACGGAAA AAGACGGAGC TTATACGGTA CACATCACAC 401 CGGGCCCGCG CACCAAAATC GCCAACGTCG GCGTCGCCAT CCTCGGCGAC 451 ATCCTTTCAG ACGGCAACCT CGCCGAATAC TACCGCAACG CGCTGGAAAA 501 CTGGCAGCAG CCGGTAGGCA GCGATTTCGA TCAGGACAGT TGGGAAAACA 551 GCAAAACTTC CGTCCTCGGC GCGGTAACGC GCAAAGCCTA CCCGCTTGCC 601 AAGCTCGGCA ATACGCAGGC GGCCGTCAAC CCCGATACCG CCACCGCCGA 651 TTTGAACGTC GTCGTGGACA GCGGCCGCCC CATCGCCTTC GGCGACTTTG 701 AAATCACCGG CACACAGCGT TACCCCGAAC AAATCGTCTC CGGCCTTGCG 751 CGTTTCCAGC CCGGTATGCC GTACGACCTC GACCTGCTGC TCGACTTCCA 801 ACAGGCGCTC GAACAAAACG GGCATTATTC CGGCGCGTCC GTACAAGCCG 851 ACTTCGACCG CCTCCAAGGC GACCGCGTCC CCGTCAAAGT CAGCGTAACC 901 GAGGTCAAAC GCCACAAACT CGAAACCGGC ATCCGCCTCG ATTCGGAATA 951 CGGTTTGGGC GGCAAAATCG CCTACGACTA TTACAACCTC TTCAACAAAG 1001 GCTATATCGG TTCGGTCGTC TGGGATATGG ACAAATACGA AACCACGCTT 1051 GCCGCCGGCA TCAGCCAGCC GCGCAACTAT CGGGGCAACT ACTGGACAAG 1101 CAACGTTTCC TACAACCGTT CGACCACCCA AAACCTCGAA AAACGCGCCT 1151 TCTCCGGCGG CGTCTGGTAT GTGCGCGACC GCGCGGGCAT CGATGCCAGG 1201 CTGGGGGCGG AATTTCTCGC AGAAGGCCGG AAAATCCCCG GCTCGGCTGT 1251 CGATTTGGGC AACAGCCACG CCACGATGCT GACCGCCTCT TGGAAACGCC 1301 AGCTGCTCAA CAACGTGCTG CATCCCGAAA ACGGCCATTA CCTCGACGGC 1351 AAAATCGGTA CGACTTTGGG CACATTCCTG TCCTCCACCG CGCTGATCCG 1401 CACCTCTGCC CGTGCAGGTT ATTTCTTCAC GCCCGAAAAC AAAAAACTCG 1451 GCACGTTCAT CATACGCGGA CAAGCGGGTT ACACCGTTGC CCGCGACAAT 1501 GCCGACGTTC CTTCAGGGCT GATGTTCCGC AGCGGCGGCG CGTCTTCCGT 1551 GCGCGGTTAC GAACTCGACA GCATCGGACT TGCCGGCCCG AACGGATCGG 1601 TCCTGCCCGA ACGCGCCCTC CTGGTGGGCA GCCTGGAATA CCAACTGCCG 1651 TTTACGCGCA CCCTTTCCGG CGCGGTGTTC CACGATATGG GCGATGCCGC 1701 CGCCAATTTC AAACGTATGA AGCTGAAACA CGGTTCGGGA CTGGGCGTGC 1751 GCTGGTTCAG CCCGCTTGCG CCGTTTTCCT TCGACATCGC CTACGGGCAC 1801 AGCGATAAGA AAATCCGCTG GCACATCAGC TTGGGAACGC GCTTCTAA This corresponds to the amino acid sequence <SEQ ID 1196; ORF 286>: m286.pep 1 MHDTRTMMIK PTALLLPALF FFPHAYAPAA DLSEΝKAAGF ALFKΝKSPDT 51 ESVKLKPKFP VLIDTQDSEI KDMVEEHLPL ITQQQEEVLD KEQTGFLAEE 101 APDΝVKTMLR SKGYFSSKVS LTEKDGAYTV HITPGPRTKI AΝVGVAILGD 151 ILSDGΝLAEY YRΝALEΝWQQ PVGSDFDQDS WEΝSKTSVLG AVTRKAYPLA 201 KLGΝTQAAVΝ PDTATADLΝV WDSGRPIAF GDFEITGTQR YPEQIVSGLA 251 RFQPGMPYDL DLLLDFQQAL EQΝGHYSGAS VQADFDRLQG DRVPVKVSVT 301 EVKRHKLETG IRLDSEYGLG GKIAYDYYΝL FΝKGYIGSW WDMDKYETTL 351 AAGISQPRΝY RGΝYWTSΝVS YΝRSTTQΝLE KRAFSGGVWY VRDRAGIDAR 401 LGAEFLAEGR KIPGSAVDLG ΝSHATMLTAS WKRQLLΝΝVL HPEΝGHYLDG 451 KIGTTLGTFL SSTALIRTSA RAGYFFTPEΝ KKLGTFIIRG QAGYTVARDΝ 501 ADVPSGLMFR SGGASSVRGY ELDSIGLAGP ΝGSVLPERAL LVGSLEYQLP 551 FTRTLSGAVF HDMGDAAAΝF KRMKLKHGSG LGVRWFSPLA PFSFDIAYGH 601 SDKKIRWHIS LGTRF* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m286/g286 95.9% identity in 293 aa overlap 10 20 30 40 50 60 m286.pep MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFKNKSPDTESVKLKPKFP I : : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I g286 MQNTGTMMIKPTALLLPALFFFPHAYAPAADLΞENKAAGFALFKSKSPDTESVKLKPKFP 10 20 30 40 50 60 70 80 90 100 110 120 m286.pep VLIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAPDNVKTMLRSKGYFSSKVS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g286 VRIDTQDΞEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAPDNVKTMLRSKGYFSSKVS 70 80 90 100 110 120 130 140 150 160 170 180 m286. pep LTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEYYRNALENWQQPVGSDFDQDS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I g286 LTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEYYRNALENWQQPVGSDFDQDS 130 140 150 160 170 180 190 200 210 220 230 240 m286.pep WENSKTSVLGAVTRKAYPLAKLGNTQAAVNPDTATADLNWVDSGRPIAFGDFEITGTQR I I I I I I I I I I I I I I I : I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g286 WENSKTSVLGAVTRKGYPLAKLGNTRAAVNPDTATADLNWVDSGRPIAFGDFEITGTQR 190 200 210 220 230 240 250 260 270 280 290 299 m286.pep YPEQIVSGLARFQPGMPYDLDLLLDFQQALEQNGHYSGASVQADFDRL-QGDRVPVKVSV llll I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I g286 YPEQTVSGLARFQPGTPYDLDLLLDFQQALEQNGHYSGASVQADFDRLPRGPRPRQSQRN 250 260 270 280 290 300 300 310 320 330 340 350 359 m286.pep TEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKGYIGSWWDMDKYETTLAAGISQPRN g286 RGQTPQTRNRHPPRFGIRFGRQNRLRLLQPLQQRLYRLGRLGYGQIRNHACRRHQPAAQL 310 320 330 340 350 360 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1197>: a286. seq 1 ATGCACGACA CCCGTACCAT GATGATTAAA CCGACCGCCC TGCTCCTGCC 51 GGCTTTATTT TTCTTTCCGC ACGCATACGC GCCTGCCGCC GACCTTTCCG 101 AAAACAAGGC GGCGGGTTTC GCATTGTTCA AAAACAAAAG CCCCGACACC 151 GAATCAGTTA AATTAAAACC CAAATTCCCC GTCCGCATCG ACACGCAGGA 201 TAGTGAAATC AAAGATATGG TCGAAGAACA CCTGCCGCTC ATCACGCAGC 251 AGCAGGAAGA AGTATTGGAC AAGGAACAGA CGGGCTTCCT CGCCGAAGAA 301 GCACCGGACA ACGTTAAAAC AATGCTCCGC AGCAAAGGCT ATTTCAGCAG 351 CAAAGTCAGC CTGACGGAAA AAGACGGAGC TTATACGGTA CACATCACAC 401 CGGGCCCGCG CACCAAAATC GCCAACGTCG GCGTCGCCAT CCTCGGCGAC 451 ATCCTTTCAG ACGGCAACCT CGCCGAATAC TACCGCAACG CGCTGGAAAA 501 CTGGCAGCAG CCGGTAGGCA GTGATTTCGA TCAGGACAGT TGGGAAAACA 551 GCAAAACTTC CGTCCTCGGC GCGGTAACGC GCAAAGCCTA CCCGCTTGCC 601 AAGCTCGGCA ACACCCGGGC GGCCGTCAAC CCCGATACCG CCACCGCCGA 651 TTTGAACGTC GTCGTGGACA GCGGCCGCCC CATCGCCTTC GGCGACTTTG 701 AAATTACCGG CACGCAGCGT TACCCCGAAC AAATCGTCTC CGGCTTGGCG 751 CGCTTCCAAC CGGGCACGCC CTACGACCTC GACCTGCTGC TCGACTTCCA 801 ACAGGCGCTC GAACAAAACG GGCATTATTC CGGCGCGTCC GTACAAGCCG 851 ACTTCGACCG CCTCCAAGGC GACCGCGTCC CCGTCAAAGT CAGCGTAACC 901 GAGGTCAAAC GCCACAAGCT CGAAACCGGC ATCCGCCTCG ATTCGGAATA 951 CGGTTTGGGC GGCAAAATCG CCTACGACTA TTACAACCTC TTCAACAAAG 1001 GCTATATCGG TTCGGTCGTC TGGGATATGG ACAAATACGA AACCACGCTT 1051 GCCGCCGGCA TCAGCCAGCC GCGCAACTAT CGGGGCAACT ACTGGACAAG 1101 CAACGTTTCC TACAACCGTT CGACCACCCA AAACCTCGAA AAACGCGCCT 1151 TCTCCGGCGG CATCTGGTAT GTGCGCGACC GCGCGGGCAT CGATGCCAGG 1201 CTGGGGGCGG AGTTTCTCGC AGAAGGCCGG AAAATCCCCG GCTCGGATAT 1251 CGATTTGGGC AACAGCCACG CCACGATGCT GACCGCCTCT TGGAAACGCC 1301 AGCTGCTCAA CAACGTGCTG CATCCCGAAA ACGGCCATTA CCTCGACGGC 1351 AAAATCGGTA CGACTTTGGG CGCATTCCTG TCCTCCACCG CGCTGATCCG 1401 CACCTCTGCC CGCGCAGGTT ATTTCTTCAC GCCCGAAAAC AAAAAACTCG 1451 GCACGTTCAT CATACGCGGA CAAGCGGGTT ACACCGTTGC CCGCGACAAT 1501 GCCAACGTTC CTTCAGGGCT GATGTTCCGC AGCGGCGGCG CGTCTTCCGT 1551 GCGCGGTTAC GAACTCGACA GCATCGGGCT TGCCGGCCCG AACGGATCGG 1601 TCCTGCCCGA ACGCGCCCTC TTGGTGGGCA GCCTGGAATA CCAACTGCCG 1651 TTTACGCGCA CCCTTTCCGG CGCGGTGTTC CACGATATGG GCGACGCCGC 1701 CGCCAATTTC AAACGTATGA AGCTGAAACA CGGTTCGGGA CTGGGCGTGC 1751 GCTGGTTCAG CCCGCTCGCG CCGTTTTCCT TCGACATCGC CTACGGGCAC 1801 AGCGACAAGA AAATCCGCTG GCACATCAGC TTGGGAACGC GCTTCTAA This corresponds to the amino acid sequence <SEQ ID 1198; ORF 286. a> a286.pep 1 MHDTRTMMIK PTALLLPALF FFPHAYAPAA DLSENKAAGF ALFKNKSPDT 51 ESVKLKPKFP VRIDTQDSEI KDMVEEHLPL ITQQQEEVLD KEQTGFLAEE 101 APDNVKTMLR SKGYFSSKVS LTEKDGAYTV HITPGPRTKI ANVGVAILGD 151 ILSDGNLAEY YRNALENWQQ PVGSDFDQDS WENSKTSVLG AVTRKAYPLA 201 KLGNTRAAVN PDTATADLNV WDSGRPIAF GDFEITGTQR YPEQIVSGLA 251 RFQPGTPYDL DLLLDFQQAL EQNGHYSGAS VQADFDRLQG DRVPVKVSVT 301 EVKRHKLETG IRLDSEYGLG GKIAYDYYNL FNKGYIGSW WDMDKYETTL 351 AAGISQPRNY RGNYWTSNVS YNRSTTQNLE KRAFSGGIWY VRDRAGIDAR 401 LGAEFLAEGR KIPGSDIDLG NSHATMLTAS WKRQLLNNVL HPENGHYLDG 451 KIGTTLGAFL SSTALIRTSA RAGYFFTPEN KKLGTFIIRG QAGYTVARDN 501 ANVPSGLMFR SGGASSVRGY ELDSIGLAGP NGSVLPERAL LVGSLEYQLP 551 FTRTLSGAVF HDMGDAAANF KRMKLKHGSG LGVRWFSPLA PFSFDIAYGH 601 SDKKIRWHIS LGTRF* m286/a286 98.7% identity in 615 aa overlap 10 20 30 40 50 60 m286.pep MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFKNKSPDTESVKLKPKFP I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a286 MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFKNKSPDTESVKLKPKFP 10 20 30 40 50 60 70 80 90 100 110 120 m286.pep VLIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAPDNVKTMLRSKGYFSSKVS I I I I I I I I I I I I I I I I I I I I I I I I I II I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I a286 VRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAPDNVKTMLRSKGYFSSKVS 70 80 90 100 110 120 130 140 150 160 170 180 m286.pep LTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEYYRNALENWQQPVGSDFDQDS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a286 LTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEYYRNALENWQQPVGSDFDQDS 130 140 150 160 170 180 190 200 210 220 230 240 m286. pep WENSKTSVLGAVTRKAYPLAKLGNTQAAVNPDTATADLNVWDSGRPIAFGDFEITGTQR I I I I I I I I I I I I I I I I I I I I I I I I I: I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a286 WENSKTSVLGAVTRKAYPLAKLGNTRAAVNPDTATADLNVWDSGRPIAFGDFEITGTQR 190 200 210 220 230 240 250 260 270 280 290 300 m286. pep YPEQIVSGLARFQPGMPYDLDLLLDFQQALEQNGHYSGASVQADFDRLQGDRVPVKVSVT lllllllllllllll I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a286 YPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHYSGASVQADFDRLQGDRVPVKVSVT 250 260 270 280 290 300 310 320 330 340 350 360 m286.pep EVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKGYIGSWWDMDKYETTLAAGISQPRNY I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a286 EVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKGYIGSWWDMDKYETTLAAGISQPRNY 310 320 330 340 350 360 370 380 390 400 410 420 m286.pep RGNYWTSNVSYNRSTTQNLEKRAFSGGVWYVRDRAGIDARLGAEFLAEGRKIPGSAVDLG I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I a286 RGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVRDRAGIDARLGAEFLAEGRKIPGSDIDLG 370 380 390 400 410 420 430 440 450 460 470 480 m286.pep NSHATMLTASWKRQLLNNVLHPENGHYLDGKIGTTLGTFLSSTALIRTSARAGYFFTPEN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I a286 NSHATMLTASWKRQLLNNVLHPENGHYLDGKIGTTLGAFLSSTALIRTSARAGYFFTPEN 430 440 450 460 470 480 490 500 510 520 530 540 m286.pep KKLGTFIIRGQAGYTVARDNADVPSGLMFRSGGASSVRGYELDSIGLAGPNGSVLPERAL I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a286 KKLGTFIIRGQAGYTVARDNANVPSGLMFRSGGASSVRGYELDSIGLAGPNGSVLPERAL 490 500 510 520 530 540 550 560 570 580 590 600 m286.pep LVGSLEYQLPFTRTLSGAVFHDMGDAAANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGH I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a286 LVGSLEYQLPFTRTLSGAVFHDMGDAAANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGH 550 560 570 580 590 600 610 m286.pep SDKKIRWHISLGTRFX I I I I I I I I I I I I I I I I a286 SDKKIRWHISLGTRFX 610 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1199>: g287.seq 1 atgtttaaac gcagtgtgat tgcaatggct tgtatttttc ccctttcagc 51 ctgtgggggc ggcggtggcg gatcgcccga tgtcaagtcg gcggacacgc 101 cgtcaaaacc ggccgccccc gttgttgctg aaaatgccgg ggaaggggtg 151 ctgccgaaag aaaagaaaga tgaggaggca gcgggcggtg cgccgcaagc 201 cgatacgcag gacgcaaccg ccggagaagg cagccaagat atggcggcag 251 tttcggcaga aaatacaggc aatggcggtg cggcaacaac ggacaacccc 301 aaaaatgaag acgcgggggc gcaaaatgat atgccgcaaa atgccgccga 351 atccgcaaat caaacaggga acaaccaacc cgccggttct tcagattccg 401 cccccgcgtc aaaccctgcc cctgcgaatg gcggtagcga ttttggaagg 451 acgaacgtgg gcaattctgt tgtgattgac ggaccgtcgc aaaatataac 501 gttgacccac tgtaaaggcg attcttgtaa tggtgataat ttattggatg 551 aagaagcacc gtcaaaatca gaatttgaaa aattaagtga tgaagaaaaa 601 attaagcgat ataaaaaaga cgagcaacgg gagaattttg tcggtttggt 651 tgctgacagg gtaaaaaagg atggaactaa caaatatatc atcttctata 701 cggacaaacc acctactcgt tctgcacggt cgaggaggtc gcttccggcc 751 gagattccgc tgattcccgt caatcaggcc gatacgctga ttgtggatgg 801 ggaagcggtc agcctgacgg ggcattccgg caatatcttc gcgcccgaag 851 ggaattaccg gtatctgact tacggggcgg aaaaattgcc cggcggatcg 901 tatgccctcc gtgtgcaagg cgaaccggca aaaggcgaaa tgcttgttgg 951 cacggccgtg tacaacggcg aagtgctgca tttccatatg gaaaacggcc 1001 gtccgtaccc gtccggaggc aggtttgccg caaaagtcga tttcggcagc 1051 aaatctgtgg acggcattat cgacagcggc gatgatttgc atatgggtac 1101 gcaaaaattc aaagccgcca tcgatggaaa cggctttaag gggacttgga 1151 cggaaaatgg cggcggggat gtttccggaa ggttttacgg cccggccggc 1201 gaggaagtgg cgggaaaata cagctatcgc ccgacagatg ctgaaaaggg 1251 cggattcggc gtgtttgccg gcaaaaaaga tcgggattga This corresponds to the amino acid sequence <SEQ ID 1200; ORF 287.ng>: g287.pep 1 MFKRSVIAMA CIFPLSACGG GGGGSPDVKS ADTPSKPAAP WAEΝAGEGV 51 LPKEKKDEEA AGGAPQADTQ DATAGEGSQD MAAVSAEΝTG ΝGGAATTDΝP 101 KΝEDAGAQΝD MPQΝAAESAΝ QTGΝΝQPAGS SDSAPASΝPA PAΝGGSDFGR 151 TNVGNSWID GPSQNITLTH CKGDSCNGDN LLDEEAPSKS EFEKLSDEEK 201 IKRYKKDEQR ENFVGLVADR VKKDGTNKYI IFYTDKPPTR SARSRRSLPA 251 EIPLIPVNQA DTLIVDGEAV SLTGHSGNIF APEGNYRYLT YGAEKLPGGS 301 YALRVQGEPA KGEMLVGTAV YNGEVLHFHM ENGRPYPSGG RFAAKVDFGS 351 KSVDGIIDSG DDLHMGTQKF KAAIDGNGFK GTWTENGGGD VSGRFYGPAG 401 EEVAGKYSYR PTDAEKGGFG VFAGKKDRD* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1201>: m287. seq 1 ATGTTTAAAC GCAGCGTAAT CGCAATGGCT TGTATTTTTG CCCTTTCAGC 51 CTGCGGGGGC GGCGGTGGCG GATCGCCCGA TGTCAAGTCG GCGGACACGC 101 TGTCAAAACC TGCCGCCCCT GTTGTTTCTG AAAAAGAGAC AGAGGCAAAG 151 GAAGATGCGC CACAGGCAGG TTCTCAAGGA CAGGGCGCGC CATCCGCACA 201 AGGCAGTCAA GATATGGCGG CGGTTTCGGA AGAAAATACA GGCAATGGCG 251 GTGCGGTAAC AGCGGATAAT CCCAAAAATG AAGACGAGGT GGCACAAAAT 301 GATATGCCGC AAAATGCCGC CGGTACAGAT AGTTCGACAC CGAATCACAC 351 CCCGGATCCG AATATGCTTG CCGGAAATAT GGAAAATCAA GCAACGGATG 401 CCGGGGAATC GTCTCAGCCG GCAAACCAAC CGGATATGGC AAATGCGGCG 451 GACGGAATGC AGGGGGACGA TCCGTCGGCA GGCGGGCAAA ATGCCGGCAA 501 TACGGCTGCC CAAGGTGCAA ATCAAGCCGG AAACAATCAA GCCGCCGGTT 551 CTTCAGATCC CATCCCCGCG TCAAACCCTG CACCTGCGAA TGGCGGTAGC 601 AATTTTGGAA GGGTTGATTT GGCTAATGGC GTTTTGATTG ACGGGCCGTC 651 GCAAAATATA ACGTTGACCC ACTGTAAAGG CGATTCTTGT AGTGGCAATA 701 ATTTCTTGGA TGAAGAAGTA CAGCTAAAAT CAGAATTTGA AAAATTAAGT 751 GATGCAGACA AAATAAGTAA TTACAAGAAA GATGGGAAGA ATGATAAATT 801 TGTCGGTTTG GTTGCCGATA GTGTGCAGAT GAAGGGAATC AATCAATATA 851 TTATCTTTTA TAAACCTAAA CCCACTTCAT TTGCGCGATT TAGGCGTTCT 901 GCACGGTCGA GGCGGTCGCT TCCGGCCGAG ATGCCGCTGA TTCCCGTCAA 951 TCAGGCGGAT ACGCTGATTG TCGATGGGGA AGCGGTCAGC CTGACGGGGC 1001 ATTCCGGCAA TATCTTCGCG CCCGAAGGGA ATTACCGGTA TCTGACTTAC 1051 GGGGCGGAAA AATTGCCCGG CGGATCGTAT GCCCTTCGTG TTCAAGGCGA 1101 ACCGGCAAAA GGCGAAATGC TTGCGGGCGC GGCCGTGTAC AACGGCGAAG 1151 TACTGCATTT CCATACGGAA AACGGCCGTC CGTACCCGAC CAGGGGCAGG 1201 TTTGCCGCAA AAGTCGATTT CGGCAGCAAA TCTGTGGACG GCATTATCGA 1251 CAGCGGCGAT GATTTGCATA TGGGTACGCA AAAATTCAAA GCCGCCATCG 1301 ATGGAAACGG CTTTAAGGGG ACTTGGACGG AAAATGGCAG CGGGGATGTT 1351 TCCGGAAAGT TTTACGGCCC GGCCGGCGAG GAAGTGGCGG GAAAATACAG 1401 CTATCGCCCG ACAGATGCGG AAAAGGGCGG ATTCGGCGTG TTTGCCGGCA 1451 AAAAAGAGCA GGATTGA This corresponds to the amino acid sequence <SEQ ID 1202; ORF 287>: m287.pep 1 MFKRSVIAMA CIFALSACGG GGGGSPDVKS ADTLSKPAAP WSEKETEAK 51 EDAPQAGSQG QGAPSAQGSQ DMAAVSEEΝT GΝGGAVTADΝ PKΝEDEVAQΝ 101 DMPQΝAAGTD SSTPΝHTPDP ΝMLAGΝMEΝQ ATDAGESSQP AΝQPDMAΝAA 151 DGMQGDDPSA GGQΝAGΝTAA QGAΝQAGΝΝQ AAGSSDPIPA SΝPAPAΝGGS 201 ΝFGRVDLAΝG VLIDGPSQΝI TLTHCKGDSC SGΝΝFLDEEV QLKSEFEKLS 251 DADKISΝYKK DGKΝDKFVGL VADSVQMKGI ΝQYIIFYKPK PTSFARFRRS 301 ARSRRSLPAE MPLIPVΝQAD TLIVDGEAVS LTGHSGΝIFA PEGΝYRYLTY 351 GAEKLPGGSY ALRVQGEPAK GEMLAGAAVY ΝGEVLHFHTE ΝGRPYPTRGR 401 FAAKVDFGSK SVDGIIDSGD DLHMGTQKFK AAIDGΝGFKG TWTEΝGSGDV 451 SGKFYGPAGE EVAGKYSYRP TDAEKGGFGV FAGKKEQD* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m287/g287 70.1% identity in 499 aa overlap 10 20 30 40 49 m287. pep MFKRSVIAMACIFALSACGGGGGGSPDVKSADTLSKPAAPWSE KETEA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I : II g287 MFKRSVIAMACIFPLSACGGGGGGSPDVKSADTPSKPAAPWAEΝAGEGVLPKEKKDEEA 10 20 30 40 50 60 50 60 70 80 90 100 109 m287. pep KEDAPQAGSQGQGAPSAQGSQDMAAVSEENTGNGGAVTADNPKNEDEVAQNDMPQNAAGT llll : I I ::: I I I I I I I I I I I I I I I I I : I : I I I I I I I I I I I I I I I I I g287 AGGAPQADTQD—ATAGEGSQDMAAVSAENTGNGGAATTDNPKNEDAGAQNDMPQNAA— 70 80 90 100 110 110 120 130 140 150 160 169 m287. pep DSSTPNHTPDPNMLAGNMENQATDAGESSQPANQPDMANAADGMQGDDPSAGGQNAGNTA g287 170 180 190 200 210 220 229 m287.pep AQGANQAGNNQAAGSSDPIPASNPAPANGGSNFGRVDLANGVLIDGPSQNITLTHCKGDS : : I I I : I I I I I I I I I I I I I I I I I I I I I : I I I : : :: I : I : I I I I I I I I I I I I I I I I I g287 -ESANQTGNNQPAGSSDSAPASNPAPANGGSDFGRTNVGNSWIDGPSQNITLTHCKGDS 120 130 140 150 160 170 230 240 250 260 270 280 289 m287.pep CSGNNFLDEEVQLKSEFEKLSDADKISNYKKDGKNDKFVGLVADSVQMKGINQYIIFYKP I : I : I : I I I I : lllllllll : I I : llll : : : I I I I I I I I : I I : I I I I I g287 CNGDNLLDEEAPSKSEFEKLSDEEKIKRYKKDEQRENFVGLVADRVKKDGTNKYIIFYTD 180 190 200 210 220 230 290 300 310 320 330 340 349 m287.pep KPTSFARFRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGNIFAPEGNYRYLT II : I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g287 KPPT RSARSRRSLPAEIPLIPVNQADTLIVDGEAVSLTGHSGNIFAPEGNYRYLT 240 250 260 270 280 290 350 360 370 380 390 400 409 m287. pep YGAEKLPGGSYALRVQGEPAKGEMLAGAAVYNGEVLHFHTENGRPYPTRGRFAAKVDFGS I I I I I I I I I I I I I I I I I I I I I I I I I : I : I I I I I I I I I I I lllllll: I I I I I I I I I I I g287 YGAEKLPGGSYALRVQGEPAKGEMLVGTAVYNGEVLHFHMENGRPYPSGGRFAAKVDFGS 300 310 320 330 340 350 410 420 430 440 450 460 469 m287. pep KSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGTWTENGSGDVSGKFYGPAGEEVAGKYSYR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I : I I I I I I I I I I I I I I I I g287 KSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGTWTENGGGDVSGRFYGPAGEEVAGKYSYR 360 370 380 390 400 410 470 480 489 m287. pep PTDAEKGGFGVFAGKKEQDX I I I I I I I I I I I I I I I I :: I I g287 PTDAEKGGFGVFAGKKDRDX 420 430 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1203>: a287.seq 1 ATGTTTAAAC GCAGTGTGAT TGCAATGGCT TGTATTGTTG CCCTTTCAGC 51 CTGTGGGGGC GGCGGTGGCG GATCGCCCGA TGTTAAGTCG GCGGACACGC 101 TGTCAAAACC TGCCGCCCCT GTTGTTACTG AAGATGTCGG GGAAGAGGTG 151 CTGCCGAAAG AAAAGAAAGA TGAGGAGGCG GTGAGTGGTG CGCCGCAAGC 201 CGATACGCAG GACGCAACCG CCGGAAAAGG CGGTCAAGAT ATGGCGGCAG 251 TTTCGGCAGA AAATACAGGC AATGGCGGTG CGGCAACAAC GGATAATCCC 301 GAAAATAAAG ACGAGGGACC GCAAAATGAT ATGCCGCAAA ATGCCGCCGA 351 TACAGATAGT TCGACACCGA ATCACACCCC TGCACCGAAT ATGCCAACCA 401 GAGATATGGG AAACCAAGCA CCGGATGCCG GGGAATCGGC ACAACCGGCA 451 AACCAACCGG ATATGGCAAA TGCGGCGGAC GGAATGCAGG GGGACGATCC 501 GTCGGCAGGG GAAAATGCCG GCAATACGGC AGATCAAGCT GCAAATCAAG 551 CTGAAAACAA TCAAGTCGGC GGCTCTCAAA ATCCTGCCTC TTCAACCAAT 601 CCTAACGCCA CGAATGGCGG CAGCGATTTT GGAAGGATAA ATGTAGCTAA 651 TGGCATCAAG CTTGACAGCG GTTCGGAAAA TGTAACGTTG ACACATTGTA 701 AAGACAAAGT ATGCGATAGA GATTTCTTAG ATGAAGAAGC ACCACCAAAA 751 TCAGAATTTG AAAAATTAAG TGATGAAGAA AAAATTAATA AATATAAAAA 801 AGACGAGCAA CGAGAGAATT TTGTCGGTTT GGTTGCTGAC AGGGTAGAAA 851 AGAATGGAAC TAACAAATAT GTCATCATTT ATAAAGACAA GTCCGCTTCA 901 TCTTCATCTG CGCGATTCAG GCGTTCTGCA CGGTCGAGGC GGTCGCTTCC 951 GGCCGAGATG CCGCTGATTC CCGTCAATCA GGCGGATACG CTGATTGTCG 1001 ATGGGGAAGC GGTCAGCCTG ACGGGGCATT CCGGCAATAT CTTCGCGCCC 1051 GAAGGGAATT ACCGGTATCT GACTTACGGG GCGGAAAAAT TGTCCGGCGG 1101 ATCGTATGCC CTCAGTGTGC AAGGCGAACC GGCAAAAGGC GAAATGCTTG 1151 CGGGCACGGC CGTGTACAAC GGCGAAGTGC TGCATTTCCA TATGGAAAAC 1201 GGCCGTCCGT CCCCGTCCGG AGGCAGGTTT GCCGCAAAAG TCGATTTCGG 1251 CAGCAAATCT GTGGACGGCA TTATCGACAG CGGCGATGAT TTGCATATGG 1301 GTACGCAAAA ATTCAAAGCC GTTATCGATG GAAACGGCTT TAAGGGGACT 1351 TGGACGGAAA ATGGCGGCGG GGATGTTTCC GGAAGGTTTT ACGGCCCGGC 1401 CGGCGAAGAA GTGGCGGGAA AATACAGCTA TCGCCCGACA GATGCGGAAA 1451 AGGGCGGATT CGGCGTGTTT GCCGGCAAAA AAGAGCAGGA TTGA This corresponds to the amino acid sequence <SEQ ID 1204; ORF 287. a>: a287.pep 1 MFKRSVIAMA CIVALSACGG GGGGSPDVKS ADTLSKPAAP WTEDVGEEV 51 LPKEKKDEEA VSGAPQADTQ DATAGKGGQD MAAVSAENTG NGGAATTDNP 101 ENKDEGPQND MPQNAADTDS STPNHTPAPN MPTRDMGNQA PDAGESAQPA 151 NQPDMANAAD GMQGDDPSAG ENAGNTADQA ANQAENNQVG GSQNPASSTN 201 PNATNGGSDF GRINVANGIK LDSGSENVTL THCKDKVCDR DFLDEEAPPK 251 SEFEKLSDEE KINKYKKDEQ RENFVGLVAD RVEKNGTNKY VIIYKDKSAS 301 SSSARFRRSA RSRRSLPAEM PLIPVNQADT LIVDGEAVSL TGHSGNIFAP 351 EGNYRYLTYG AEKLSGGSYA LSVQGEPAKG EMLAGTAVYN GEVLHFHMEN 401 GRPSPSGGRF AAKVDFGSKS VDGIIDSGDD LHMGTQKFKA VIDGNGFKGT 451 WTENGGGDVS GRFYGPAGEE VAGKYSYRPT DAEKGGFGVF AGKKEQD* m287/a287 77.2% identity in 501 aa overlap 10 20 30 40 49 m287.pep MFKRSVIAMACIFALSACGGGGGGSPDVKSADTLSKPAAPWSE KETEA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I: I I : II a287 MFKRSVIAMACIVALSACGGGGGGSPDVKSADTLSKPAAPWTEDVGEEVLPKEKKDEEA 10 20 30 40 50 60 50 60 70 80 90 100 109 m287. pep KEDAPQAGSQGQGAPSAQGSQDMAAVSEENTGNGGAVTADNPKNEDEVAQNDMPQNAAGT llll : I I ::: I: I I I I I I I I I I I I I I I : I : I I I : I : I I lllllllll I a287 VSGAPQADTQ--DATAGKGGQDMAAVSAENTGNGGAATTDNPENKDEGPQNDMPQNAADT 70 80 90 100 110 110 120 130 140 150 160 169 m287.pep DSSTPNHTPDPNMLAGNMENQATDAGESSQPANQPDMANAADGMQGDDPSAGGQNAGNTA lllllllll III : : I III I I I I I : I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I a287 DSSTPNHTPAPNMPTRDMGNQAPDAGESAQPANQPDMANAADGMQGDDPSAG-ENAGNTA 120 130 140 150 160 170 170 180 190 200 210 220 229 m287.pep AQGANQAGNNQAAGSSDPIPASNPAPANGGSNFGRVDLANGVLIDGPSQNITLTHCKGDS I : I I I I I I I : : I I : : I : : I I : I I I I : I I I : : : I I I : : I : I : I : I I I I I I a287 DQAANQAENNQVGGSQNPASSTNPNATNGGSDFGRINVANGIKLDSGSENVTLTHCKDKV 180 190 200 210 220 230 230 240 250 260 270 280 289 m287. pep CSGNNFLDEEVQLKSEFEKLSDADKISNYKKDGKNDKFVGLVADSVQMKGINQYIIFYKP I : : I I I I I : lllllllll : I |:: I I I I : :: I I I I I I I I : : I I : I : I : I I a287 CD-RDFLDEEAPPKSEFEKLSDEEKINKYKKDEQRENFVGLVADRVEKNGTNKYVIIYKD 240 250 260 270 280 290 290 300 310 320 330 340 m287.pep KP--TSFARFRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGNIFAPEGNYRY I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a287 KSASSSSARFRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGNIFAPEGNYRY 300 310 320 330 340 350 350 360 370 380 390 400 m287. pep LTYGAEKLPGGΞYALRVQGEPAKGEMLAGAAVYNGEVLHFHTENGRPYPTRGRFAAKVDF llllllll llllll I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I : lllllllll a287 LTYGAEKLSGGSYALSVQGEPAKGEMLAGTAVYNGEVLHFHMENGRPSPSGGRFAAKVDF 360 370 380 390 400 410 410 420 430 440 450 460 m287.pep GSKSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGTWTENGSGDVSGKFYGPAGEEVAGKYS I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I : I I I I I : I I I I I I I I I I I I I I a287 GSKSVDGIIDSGDDLHMGTQKFKAVIDGNGFKGTWTENGGGDVSGRFYGPAGEEVAGKYS 420 430 440 450 460 470 470 480 489 m287.pep YRPTDAEKGGFGVFAGKKEQDX I I I I I I I I I I I I I I I I I I I I II a287 YRPTDAEKGGFGVFAGKKEQDX 480 490 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1205>: g288.seq 1 atgcacaccg gacaggcggt aagccgggtt ctgtctcgga cagtcattcc 51 tctaggcata ccgttgccgg tatgctcaag caacctaccc gaacgctcgg 101 cgggcagcgt cattgcgttc tgtttggtct tgctccgaat ggggtttggc 151 ctgccgcata ttgttaccaa atgcgcggtg cgcccttacc gcaccttttc 201 acccttgcct gtgctgccaa agcagccatc ggcggttttg ctttctgttc 251 cactttccgt cgcgttaccg cgcccggccg ttaaccggca ttctaccctg 301 cggagcccgg actttcctcc ccgtatgcct tacgcgatac gcggcgactg 351 tctgcccgtc ccgtgtgcgg cgcggattat aacacgaaac gcaaaaatgc 401 cgtctgaaac ggtacaggtt tcagacggca tacagcctaa actacacacc 451 ctgtttcagg ctggcttcga tgaagccgtc caagtcgccg tccaatacgg 501 ctttgtggtt gccgacttcg tagcctgtac gcaagtcttt gatgcgtga This corresponds to the amino acid sequence <SEQ ID 1206; ORF 288.ng>: g288.pep 1 MHTGQAVSRV LSRTVIPLGI PLPVCSSΝLP ERSAGSVIAF CLVLLRMGFG 51 LPHIVTKCAV RPYRTFSPLP VLPKQPSAVL LSVPLSVALP RPAVΝRHSTL 101 RSPDFPPRMP YAIRGDCLPV PCAARIITRΝ AKMPSETVQV SDGIQPKLHT 151 LFQAGFDEAV QVAVQYGFW ADFVACTQVF DA* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1207>: m288.seq 1 ATGCACACCG GACAGGCGGT AAGCCGGGTT CTGTCTCGGA CAGTCATTCC 51 TCTAGGCATA CCGTTACCGG TATGCTCAAG CAACCTACCC GAACGCTCGG 101 CGGGCAGCGT CATTGCGTTC TGTTTGGTCT TGCTCCGAAT GGGGTTTGGC 151 CTGCCGCATA TTGTTACCAA ATGCGCGGTG CGCCCTTACC GCACCTTTTC 201 ACCCTTACCT GTGCTGCCAA AGCAGCCATC GGCGGTTTTG CTTTCTGTTC 251 CACTTTCCGT CGCGTTACCG CGCCCGGCCG TTAACCGGCA TTCTACCCTG 301 CGGAGCCCGG ACTTTCCTCC CCGTATGCCT TACGCGATAC GCGGCGACTG 351 TCTGCCCGTC CCGTGTGCGG CGCGGATTAT AACACGAAAC ACAAAAATGC 401 CGTCTGAAAC GGTACAGGTT TCAGACGGCA TACAGCCTAA ACTACACGCC 451 CTGTTTCAGG CTGGCTTCGA TGAAGCCGTC CAAGTCGCCA TCCAATACGG 501 CTTTGGTGTT GCCGACTTCG TAGCCTGTAC GCAAGTCTTT GATACGTGA This corresponds to the amino acid sequence <SEQ ID 1208; ORF 288>: m288.pep 1 MHTGQAVSRV LSRTVIPLGI PLPVCSSΝLP ERSAGSVIAF CLVLLRMGFG 51 LPHIVTKCAV RPYRTFSPLP VLPKQPSAVL LSVPLSVALP RPAVΝRHSTL 101 RSPDFPPRMP YAIRGDCLPV PCAARIITRΝ TKMPSETVQV SDGIQPKLHA 151 LFQAGFDEAV QVAIQYGFGV ADFVACTQVF DT* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m288/g288 97.8% identity in 181 aa overlap 10 20 30 40 50 60 m288. pep MHTGQAVSRVLSRTVIPLGIPLPVCSSΝLPERSAGSVIAFCLVLLRMGFGLPHIVTKCAV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g288 MHTGQAVSRVLSRTVIPLGIPLPVCSSΝLPERSAGSVIAFCLVLLRMGFGLPHIVTKCAV 10 20 30 40 50 60 70 80 90 100 110 120 m288.pep RPYRTFSPLPVLPKQPSAVLLSVPLSVALPRPAVΝRHSTLRSPDFPPRMPYAIRGDCLPV I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g288 RPYRTFSPLPVLPKQPSAVLLSVPLSVALPRPAVΝRHSTLRSPDFPPRMPYAIRGDCLPV 70 80 90 100 110 120 130 140 150 160 170 180 m288. pep PCAARIITRΝTKMPSETVQVSDGIQPKLHALFQAGFDEAVQVAIQYGFGVADFVACTQVF I I I I I I I I I I : I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I : I I I I I I I I I I I I I I I g288 PCAARIITRΝAKMPSETVQVSDGIQPKLHTLFQAGFDEAVQVAVQYGFWADFVACTQVF 130 140 150 160 170 180 m288.pep DTX I : I g288 DAX The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1209>: a288.seq 1 ATGCACACCG GACAGGCGGT AAGCCGGGTT CTGTCTCGGA CAGTCATTCC 51 TCTAGGCATA CCGTTGCCGG TATGCTCAAG CAACCTACCC GAACGCTCGG 101 CGGGCAGCGT CATTGCGTTC TGTTTGGTCT TGCTCCGAAT GGGGTTTGGC 151 CTGCCGCATA TTGTTACCAA ATGCGCGGTG CGCCCTTACC GCACCTTTTC 201 ACCCTTGCCT GTGCTGCCAA AGCAGCCATC GGCGGTTTTG CTTTCTGTTC 251 CACTTTCCGT CGCGTTACCG CGCCCGGCCG TTAACCGGCA TTCTACCCTG 301 CGGAGCCCGG ACTTTCCTCC CCGTATGCCT TACGCGATAC GCGGCGACTG 351 TCTGCCCGTC CCGTGTGCGG CGCGGATTAT AACACGAAAC GCAAAAATGC 401 CGTCTGAAAC GGTACAGGTT TCAGACGGCA TACAGCCTAA ACTACACGCC 451 CTGTTTCAGG CTGGCTTCGA TAAAGCCGTC CAAGTCGCCG TCCAATACGG 501 CTTTGGTGTT GCCGACTTCG TAGCCTGTGC GCAAGTCTTT AATGCGTGA This corresponds to the amino acid sequence <SEQ ID 1210; ORF 288. a>: a288.pep 1 MHTGQAVSRV LSRTVIPLGI PLPVCSSΝLP ERSAGSVIAF CLVLLRMGFG 51 LPHIVTKCAV RPYRTFSPLP VLPKQPSAVL LSVPLSVALP RPAVΝRHSTL 101 RSPDFPPRMP YAIRGDCLPV PCAARIITRΝ AKMPSETVQV SDGIQPKLHA 151 LFQAGFDKAV QVAVQYGFGV ADFVACAQVF ΝA* m288/a288 97.2% identity in 181 aa overlap 10 20 30 40 50 60 m288.pep MHTGQAVSRVLSRTVIPLGIPLPVCSSΝLPERSAGSVIAFCLVLLRMGFGLPHIVTKCAV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I a288 MHTGQAVSRVLSRTVIPLGIPLPVCSSΝLPERSAGSVIAFCLVLLRMGFGLPHIVTKCAV 10 20 30 40 50 60 70 80 90 100 110 120 m288.pep RPYRTFSPLPVLPKQPSAVLLSVPLSVALPRPAVΝRHSTLRSPDFPPRMPYAIRGDCLPV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a288 RPYRTFSPLPVLPKQPSAVLLSVPLSVALPRPAVΝRHSTLRSPDFPPRMPYAIRGDCLPV 70 80 90 100 110 120 130 140 150 160 170 180 m288.pep PCAARIITRΝTKMPSETVQVSDGIQPKLHALFQAGFDEAVQVAIQYGFGVADFVACTQVF I I I I I I I I M : I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I : I I I I I I I I I I I I : I I I a288 PCAARIITRNAKMPSETVQVSDGIQPKLHALFQAGFDKAVQVAVQYGFGVADFVACAQVF 130 140 150 160 170 180 m288.pep DTX a288 NAX The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1211>: g290.seq 1 atggcaaaaa tgatgaaatg ggcggctgtt gcggcggtcg cggcggcagc 51 ggtttggggc ggatggtctt atctgaagcc cgaaccgcag gctgcttata 101 ttacggaagc ggtcaggcgc ggcgatatca gccggacggt ttccgcgacg 151 ggcgagattt cgccgtccaa cctggtatcg gtcggcgcgc aggcttcggg 201 gcagattaaa aagctttatg tcaaactcgg gcaacaggtc aaaaagggcg 251 atttgattgc ggaaatcaat tcgaccacgc agaccaacac gatcgatatg 301 gaaaaatcca aattggaaac gtatcaggcg aagctggtgt ccgcacagat 351 tgcattgggc agcgcggaaa aaaaatataa gcgtcaggcg gcgttgtgga 401 aggatgatgc gacctctaaa gaagatttgg aaagcgcgca ggatgcgctt 451 gccgccgcca aagccaatgt tgccgagttg aaggctttaa tcagacagag 501 caaaatttcc atcaataccg ccgagtcgga tttgggctac acgcgcatta 551 ccgcgacgat ggacggcacg gtggtggcga ttcccgtgga agaggggcag 601 actgtgaacg cggcgcagtc tacgccgacg attgtccaat tggcgaatct 651 ggatatgatg ttgaacaaaa tgcagattgc cgagggcgat attaccaagg 701 tgaaggcggg gcaggatatt tcgtttacga ttttgtccga accggatacg 751 ccgattaagg cgaagctcga cagcgtcgac cccgggctga ccacgatgtc 801 gtcgggcggc tacaacagca gtacggatac ggcttccaat gcggtctatt 851 attatgcccg ttcgtttgtg ccgaatccgg acggcaaact cgccacgggg 901 atgacgacgc agaatacggt tgaaatcgac ggtgtgaaaa atgtgttgct 951 tattccgtcg ctgaccgtga aaaatcgcgg cggcaaggcg ttcgtacgcg 1001 tgttgggtgc ggacggcaag gcagtggaac gcgaaatccg gaccggtatg 1051 aaagacagta tgaataccga agtgaaaagc gggttgaaag agggggacaa 1101 agtggtcatc tccgaaataa ccgccgccga gcagcaggaa agcggcgaac 1151 gcgccctagg cggcccgccg cgccgataa This corresponds to the amino acid sequence <SEQ ID 1212; ORF 290.ng>: g290.pep 1 MAKMMKWAAV AAVAAAAVWG GWSYLKPEPQ AAYITEAVRR GDISRTVSAT 51 GEISPSΝLVS VGAQASGQIK KLYVKLGQQV KKGDLIAEIΝ STTQTΝTIDM 101 EKSKLETYQA KLVSAQIALG SAEKKYKRQA ALWKDDATSK EDLESAQDAL 151 AAAKAΝVAEL KALIRQSKIS IΝTAESDLGY TRITATMDGT WAIPVEEGQ 201 TVΝAAQSTPT IVQLAΝLDMM LΝKMQIAEGD ITKVKAGQDI SFTILSEPDT 251 PIKAKLDSVD PGLTTMSSGG YΝSSTDTASΝ AVYYYARSFV PΝPDGKLATG 301 MTTQΝTVEID GVKΝVLLIPS LTVKΝRGGKA FVRVLGADGK AVEREIRTGM 351 KDSMΝTEVKS GLKEGDKWI SEITAAEQQE SGERALGGPP RR* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1213>: m290.seq (partial) 1 .. GTATCGGTCG GCGCGCAGGC ATCGGGGCAG ATTAAGATAC TTTATGTCAA 51 ACTCGGGCAA CAGGTTAAAA AGGGCGATTT GATTGCGGAA ATCAATTCGA 101 CCTCGCAGAC CAATACGCTC AATACGGAAA AATCCAAGTT GGAAACGTAT 151 CAGGCGAAGC TGGTGTCGGC ACAGATTGCA TTGGGCAGCG CGGAGAAGAA 201 ATATAAGCGT CAGGCGGCGT TATGGAAGGA AAACGCGACT TCCAAAGAGG 251 ATTTGGAAAG CGCGCAGGAT GCGTTTGCCG CCGCCAAAGC CAATGTTGCC 301 GAGCTGAAGG CTTTAATCAG ACAGAGCAAA ATTTCCATCA ATACCGCCGA 351 GTCGGAATTG GGCTACACGC GCATTACCGC AACGATGGAC GGCACGGTGG 401 TGGCGATTCT CGTGGAAGAG GGGCAGACTG TGAACGCGGC GCAGTCTACG 451 CCGACGATTG TCCAATTGGC GAATCTGGAT ATGATGTTGA ACAAAATGCA 501 GATTGCCGAG GGCGATATTA CCAAGGTGAA GGCGGGGCAG GATATTTCGT 551 TTACGATTTT GTCCGAACCG GATACGCCGA TTAAGGCGAA GCTCGACAGC 601 GTCGACCCCG GGCTGACCAC GATGTCGTCG GGCGGTTACA ACAGCAGTAC 651 GGATACGGCT TCCAATGCGG TCTACTATTA TGCCCGTTCG TTTGTGCCGA 701 ATCCGGACGG CAAACTCGCC ACGGGGATGA CGACGCAGAA TACGGTTGAA 751 ATCGACGGCG TGAAAAATGT GCTGATTATT CCGTCGCTGA CCGTGAAAAA 801 TCGCGGCGGC AAGGCGTTTG TGCGCGTGTT GGGTGCGGAC GGCAAGGCGG 851 CGGAACGCGA AATCCGGACC GGTATGAGAG ACAGTATGAA TACCGAAGTA 901 AAAAGCGGGT TGAAAGAGGG GGACAAAGTG GTCATCTCCG AAATAACCGC 951 CGCCGAGCAA CAGGAAAGCG GCGAACGCGC CCTAGGCGGC CCGCCGCGCC 1001 GATAA This corresponds to the amino acid sequence <SEQ ID 1214; ORF 290>: m290.pep (partial) 1 ..VSVGAQASGQ IKILYVKLGQ QVKKGDLIAE INSTSQTNTL NTEKSKLETY 51 QAKLVSAQIA LGSAEKKYKR QAALWKENAT SKEDLESAQD AFAAAKANVA 101 ELKALIRQSK ISINTAESEL GYTRITATMD GTWAILVEE GQTVNAAQST 151 PTIVQLANLD MMLNKMQIAE GDITKVKAGQ DISFTILSEP DTPIKAKLDS 201 VDPGLTTMSS GGYNSSTDTA SNAVYYYARS FVPNPDGKLA TGMTTQNTVE 251 IDGVKNVLII PSLTVKNRGG KAFVRVLGAD GKAAEREIRT GMRDSMNTEV 301 KSGLKEGDKV VISEITAAEQ QESGERALGG PPRR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m290/g290 96.1% identity in 334 aa overlap 10 20 30 m290. pep VSVGAQASGQIKILYVKLGQQVKKGDLIAE I I I I I I I I I I I I lllllllllllllllll g290 PQAAYITEAVRRGDISRTVSATGEISPSΝLVSVGAQASGQIKKLYVKLGQQVKKGDLIAE 30 40 50 60 70 80 40 50 60 70 80 90 m290.pep IΝSTSQTΝTLΝTEKSKLETYQAKLVSAQIALGSAEKKYKRQAALWKEΝATSKEDLESAQD I I I I : I I I I :: I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I :: I I I I I I I I I I I I g290 IΝSTTQTΝTIDMEKSKLETYQAKLVSAQIALGSAEKKYKRQAALWKDDATSKEDLESAQD 90 100 110 120 130 140 100 110 120 130 140 150 m290.pep AFAAAKAΝVAELKALIRQSKISIΝTAESELGYTRITATMDGTWAILVEEGQTVΝAAQST I : I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g290 ALAAAKAΝVAELKALIRQSKISIΝTAESDLGYTRITATMDGTWAIPVEEGQTVΝAAQST 150 160 170 180 190 200 160 170 180 190 200 210 m290.pep PTIVQLAΝLDMMLΝKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g290 PTIVQLAΝLDMMLΝKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSS 210 220 230 240 250 260 220 230 240 250 260 270 m290. pep GGYΝSSTDTASΝAVYYYARSFVPΝPDGKLATGMTTQΝTVEIDGVKΝVLIIPSLTVKΝRGG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I g290 GGYΝSSTDTASΝAVYYYARSFVPΝPDGKLATGMTTQΝTVEIDGVKΝVLLIPSLTVKΝRGG 270 280 290 300 310 320 280 290 300 310 320 330 m290.pep KAFVRVLGADGKAAEREIRTGMRDSMΝTEVKSGLKEGDKWIΞEITAAEQQESGERALGG I I I I I I I I I I I I I : I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g290 KAFVRVLGADGKAVEREIRTGMKDSMΝTEVKSGLKEGDKWISEITAAEQQESGERALGG 330 340 350 360 370 380 m290.pep PPRRX I I I I I g290 PPRRX 390 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1215>: a290.seq 1 ATGGCAAAAA TGATGAAATG GGCGGCTGTT GCGGCGGTCG CGGCGGCAGC 51 GGTTTGGGGC GGATGGTCTT ATCTGAAGCC CGAGCCGCAG GCTGCTTATA 101 TTACGGAAAC GGTCAGGCGC GGCGACATCA GCCGGACGGT TTCTGCAACA 151 GGGGAGATTT CGCCGTCCAA CCTGGTATCG GTCGGCGCGC AGGCATCGGG 201 GCAGATTAAG AAACTTTATG TCAAACTCGG GCAACAGGTT AAAAAGGGCG 251 ATTTGATTGC GGAAATCAAT TCGACCTCGC AGACCAATAC GCTCAATACG 301 GAAAAATCCA AATTGGAAAC GTATCAGGCG AAGCTGGTGT CGGCACAGAT 351 TGCATTGGGC AGCGCGGAGA AGAAATATAA GCGTCAGGCG GCGTTGTGGA 401 AGGATGATGC GACCGCTAAA GAAGATTTGG AAAGCGCACA GGATGCGCTT 451 GCCGCCGCCA AAGCCAATGT TGCCGAGCTG AAGGCTCTAA TCAGACAGAG 501 CAAAATTTCC ATCAATACCG CCGAGTCGGA ATTGGGCTAC ACGCGCATTA 551 CCGCAACGAT GGACGGCACG GTGGTGGCGA TTCTCGTGGA AGAGGGGCAG 601 ACTGTGAACG CGGCGCAGTC TACGCCGACG ATTGTCCAAT TGGCGAATCT 651 GGATATGATG TTGAACAAAA TGCAGATTGC CGAGGGCGAT ATTACCAAGG 701 TGAAGGCGGG GCAGGATATT TCGTTTACGA TTTTGTCCGA ACCGGATACG 751 CCGATTAAGG CGAAGCTCGA CAGCGTCGAC CCCGGGCTGA CCACGATGTC 801 GTCGGGCGGC TACAACAGCA GTACGGATAC GGCTTCCAAT GCGGTCTACT 851 ATTATGCCCG TTCGTTTGTG CCGAATCCGG ACGGCAAACT CGCCACGGGG 901 ATGACGACGC AGAATACGGT TGAAATCGAC GGTGTGAAAA ATGTGCTGAT 951 TATTCCGTCG CTGACCGTGA AAAATCGCGG CGGCAGGGCG TTTGTGCGCG 1001 TGTTGGGTGC AGACGGCAAG GCGGCGGAAC GCGAAATCCG GACCGGTATG 1051 AGAGACAGTA TGAATACCGA AGTAAAAAGC GGGTTGAAAG AGGGGGACAA 1101 AGTGGTCATC TCCGAAATAA CCGCCGCCGA GCAGCAGGAA AGCGGCGAAC 1151 GCGCCCTAGG CGGCCCGCCG CGCCGATAA This corresponds to the amino acid sequence <SEQ ID 1216; ORF 290. a>: a290.pep 1 MAKMMKWAAV AAVAAAAVWG GWSYLKPEPQ AAYITETVRR GDISRTVSAT 51 GEISPSΝLVS VGAQASGQIK KLYVKLGQQV KKGDLIAEIΝ STSQTΝTLΝT 101 EKSKLETYQA KLVSAQIALG SAEKKYKRQA ALWKDDATAK EDLESAQDAL 151 AAAKAΝVAEL KALIRQSKIS IΝTAESELGY TRITATMDGT WAILVEEGQ 201 TVΝAAQSTPT IVQLAΝLDMM LΝKMQIAEGD ITKVKAGQDI SFTILSEPDT 251 PIKAKLDSVD PGLTTMSSGG YΝSSTDTASΝ AVYYYARSFV PΝPDGKLATG 301 MTTQΝTVEID GVKΝVLIIPS LTVKΝRGGRA FVRVLGADGK AAEREIRTGM 351 RDSMΝTEVKS GLKEGDKWI SEITAAEQQE SGERALGGPP RR* m290/a290 98.2% identity in 334 aa overlap 10 20 30 m290. pep VSVGAQASGQIKILYVKLGQQVKKGDLIAE I I I II I I I I I I I lllllllllllllllll a290 PQAAYITETVRRGDISRTVSATGEISPSΝLVSVGAQASGQIKKLYVKLGQQVKKGDLIAE 30 40 50 60 70 80 40 50 60 70 80 90 m290. pep IΝSTSQTΝTLΝTEKSKLETYQAKLVSAQIALGSAEKKYKRQAALWKEΝATSKEDLESAQD I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I :: I I : I I I I I I I I I a290 IΝSTSQTΝTLΝTEKSKLETYQAKLVSAQIALGSAEKKYKRQAALWKDDATAKEDLESAQD 90 100 110 120 130 140 100 110 120 130 140 150 m290. pep AFAAAKAΝVAELKALIRQSKISIΝTAESELGYTRITATMDGTWAILVEEGQTVΝAAQST I : I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a290 ALAAAKAΝVAELKALIRQSKISIΝTAESELGYTRITATMDGTWAILVEEGQTVΝAAQST 150 160 170 180 190 200 160 170 180 190 200 210 PTIVQLAΝLDMMLΝKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a290 PTIVQLAΝLDMMLΝKM Q IAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSS 210 220 230 240 250 260 220 230 240 250 260 270 m290.pep GGYNSSTDTASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I a290 GGYNSSTDTASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGG 270 280 290 300 310 320 280 290 300 310 320 330 m290.pep KAFVRVLGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKWISEITAAEQQESGERALGG : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a290 RAFVRVLGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKWISEITAAEQQESGERALGG 330 340 350 360 370 380 m290.pep PPRRX Mill a290 PPRRX 390 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1217>: g292.seq 1 atgaaaacca agttaatcaa aatcttgacc ccctttaccg tcctgccgct 51 gctggcttgc gggcaaacgc ccgtttccaa tgccaacgcc gaatccgccg 101 tcaaagccga atccgccggc aaatccgttg ccgcttcttt gaaagcgcgt 151 ttggaaaaaa cctattccgc ccaagatttg aaagtgttga gcgtcagcga 201 aacaccggtc aaaggcattt acgaagtcgt cgtcagcggc aggcagatta 251 tctacaccga tgccgaaggc ggctatatgt tcgtcggcga actcatcaac 301 atcgacacgc gcaaaaacct gaccgaagaa cgcgccgccg atttgaacaa 351 aatcgacttc gcctccctgc ctttggacaa agccatcaaa gaagtacgcg 401 gcaacggcaa gctgaaagtc gccgtcttct ccgaccccga ttgtccgttc 451 tgcaaacgct tggaacatga gtttgaaaaa atgaccgacg tgacggttta 501 cagctttatg atgcccattg ccggcctgca cccagatgcc gcgcgcaagg 551 cgcaaatctt atggtgtcag cccgaccgtg ccaaagcgtg gacggattgg 601 atgcgtaaag gcaaattccc ggtcggcggc agcatctgcg acaatcccgt 651 cgcggaaacc acttccttgg gcgaacagtt cggcttcaac ggcacgccga 701 cccttcgtct tccccaacgg gcgcacccaa agcggttaca gcccgatgcc 751 ccaactggag gaaatcatcc gcaaaaacca gcagtaaacc cgcaatga This corresponds to the amino acid sequence <SEQ ID 1218; ORF 292.ng>: g292.pep 1 MKTKLIKILT PFTVLPLLAC GQTPVSΝAΝA ESAVKAESAG KSVAASLKAR 51 LEKTYSAQDL KVLSVSETPV KGIYEVWSG RQIIYTDAEG GYMFVGELIΝ 101 IDTRKΝLTEE RAADLΝKIDF ASLPLDKAIK EVRGΝGKLKV AVFSDPDCPF 151 CKRLEHEFEK MTDVTVYSFM MPIAGLHPDA ARKAQILWCQ PDRAKAWTDW 201 MRKGKFPVGG SICDΝPVAET TSLGEQFGFΝ GTPTLRLPQR AHPKRLQPDA 251 PTGGΝHPQKP AVΝPQ* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1219>: m292.seq 1 ATGAAAACCA AGTTAATCAA AATCTTGACC CCCTTTACCG TCCTCCCGCT 51 GCTGGCTTGC GGGCAAACGC CCGTTTCCAA TGCCAACGCC GAACCCGCCG 101 TCAAAGCCGA GTCCGCCGGC AAATCCGTTG CCGCCTCTTT GAAAGCGCGT 151 TTGGAAAAAA CCTATTCCGC CCAAGATTTG AAAGTGTTGA GCGTCAGCGA 201 AACACCGGTC AAAGGCATTT ACGAAGTCGT CGTCAGCGGC AGGCAGATTA 251 TCTACACCGA TGCCGAAGGC GGCTATATGT TCGTCGGCGA ACTCATCAAC 301 ATCGACACGC GCAAAAACCT GACCGAAGAA CGCGCCGCCG ATTTGAACAA 351 AATCGACTTC GCCTCCCTGC CTTTGGACAA AGCCATCAAA GAAGTGCGCG 401 GCAACGGCAA GCTGAAAGTC GCCGTCTTCT CCGACCCCGA TTGTCCGTTC 451 TGCAAACGCT TGGAACACGA GTTTGAAAAA ATGACCGACG TGACGGTTTA 501 CAGCTTTATG ATGCCCATTG CCGGCCTGCA CCCCGATGCC GCGCGCAAGG 551 CGCAAATCTT ATGGTGTCAG CCCGACCGCG CCAAAGCGTG GACGGATTGG 601 ATGCGTAAAG GCAAATTCCC GGTCGGCGGC AGCATCTGCG ACAATCCCGT 651 CGCGGAAACC ACTTCCTTGG GCGAACAATT CGGCTTCAAC GGCACGCCGA 701 CCCTCGTCTT CCCCAACGGG CGCAGCCAAA GCGGCTACAG CCCGATGCCC 751 CAACTGGAGG AAATCATCCG CAAAAATCAA TAA This corresponds to the amino acid sequence <SEQ ID 1220; ORF 292>: m292.pep 1 MKTKLIKILT PFTVLPLLAC GQTPVSNANA EPAVKAESAG KSVAASLKAR 51 LEKTYSAQDL KVLSVSETPV KGIYEVWSG RQIIYTDAEG GYMFVGELIN 101 IDTRKNLTEE RAADLNKIDF ASLPLDKAIK EVRGNGKLKV AVFSDPDCPF 151 CKRLEHEFEK MTDVTVYSFM MPIAGLHPDA ARKAQILWCQ PDRAKAWTDW 201 MRKGKFPVGG SICDNPVAET TSLGEQFGFN GTPTLVFPNG RSQSGYSPMP 251 QLEEIIRKNQ * Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m292/g292 98.7% identity in 238 aa overlap 10 20 30 40 50 60 m292. pep MKTKLIKILTPFTVLPLLACGQTPVSΝAΝAEPAVKAESAGKSVAASLKARLEKTYSAQDL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g292 MKTKLIKILTPFTVLPLLACGQTPVSΝAΝAESAVKAESAGKSVAASLKARLEKTYSAQDL 10 20 30 40 50 60 70 80 90 100 110 120 m292.pep KVLSVSETPVKGIYEVWSGRQIIYTDAEGGYMFVGELIΝIDTRKΝLTEERAADLΝKIDF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g292 KVLSVSETPVKGIYEVWSGRQIIYTDAEGGYMFVGELIΝIDTRKΝLTEERAADLΝKIDF 70 80 90 100 110 120 130 140 150 160 170 180 m292 . pep ASLPLDKAIKEVRGΝGKLKVAVFSDPDCPFCKRLEHEFEKMTDVTVYSFMMPIAGLHPDA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g292 ASLPLDKAIKEVRGΝGKLKVAVFSDPDCPFCKRLEHEFEKMTDVTVYSFMMPIAGLHPDA 130 140 150 160 170 180 190 200 210 220 230 240 m292.pep ARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDΝPVAETTSLGEQFGFΝGTPTLVFPΝG I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I : g292 ARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDΝPVAETTSLGEQFGFΝGTPTLRLPQR 190 200 210 220 230 240 250 260 m292.pep RSQSGYSPMPQLEEIIRKΝQX g292 AHPKRLQPDAPTGGΝHPQKPAVΝPQX 250 260 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1221>: a292.seq 1 ATGAAAACCA AGTTAATCAA AATCTTGACC CCCTTTACCG TCCTCCCGCT 51 GCTGGCTTGC GGGCAAACGC CCGTTTCCAA TGCCAACGCC GAACCCGCCG 101 TCAAAGCCGA GTCCGCCGGC AAATCCGTTG CCGCCTCTTT GAAAGCGCGT 151 TTGGAAAAAA CCTATTCCGC CCAAGATTTG AAAGTGTTGA GCGTCAGCGA 201 AACACCGGTC AAAGGCATTT ACGAAGTCGT CGTCAGCGGC AGGCAGATTA 251 TCTACACCGA TGCCGAAGGC GGCTATATGT TCGTCGGCGA ACTCATCAAC 301 ATCGACACGC GCAAAAACCT GACCGAAGAA CGCGCCGCCG ATTTGAACAA 351 AATCGACTTC GCCTCCCTGC CTTTGGACAA AGCCATCAAA GAAGTGCGCG 401 GCAACGGCAA GCTGAAAGTC GCCGTCTTCT CCGACCCCGA TTGTCCGTTC 451 TGCAAACGCT TGGAACACGA GTTTGAAAAA ATGACCGACG TGACGGTTTA 501 CAGCTTTATG ATGCCCATTG CCGGCCTGCA CCCCGATGCC GCGCGCAAGG 551 CGCAAATCTT ATGGTGTCAG CCCGACCGCG CCAAAGCGTG GACGGATTGG 601 ATGCGTAAAG GCAAATTCCC GGTCGGCGGC AGCATCTGCG ACAATCCCGT 651 CGCGGAAACC ACTTCCTTGG GCGAACAATT CGGCTTCAAC GGCACGCCGA 701 CCCTCGTCTT CCCCAACGGG CGCAGCCAAA GCGGCTACAG CCCGATGCCC 751 CAACTGGAGG AAATCATCCG CAAAAATCAA TAA This corresponds to the amino acid sequence <SEQ ID 1222; ORF 292. a>: a292.pep 1 MKTKLIKILT PFTVLPLLAC GQTPVSNANA EPAVKAESAG KSVAASLKAR 51 LEKTYSAQDL KVLSVSETPV KGIYEVWSG RQIIYTDAEG GYMFVGELIN 101 IDTRKNLTEE RAADLNKIDF ASLPLDKAIK EVRGNGKLKV AVFSDPDCPF 151 CKRLEHEFEK MTDVTVYSFM MPIAGLHPDA ARKAQILWCQ PDRAKAWTDW 201 MRKGKFPVGG SICDNPVAET TSLGEQFGFN GTPTLVFPNG RSQSGYSPMP 251 QLEEIIRKNQ * m292/a292 100.0% identity in 260 aa overlap 10 20 30 40 50 60 m292 •pep MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAVKAESAGKSVAASLKARLEKTYSAQDL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a292 MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAVKAESAGKSVAASLKARLEKTYSAQDL 10 20 30 40 50 60 70 80 90 100 110 120 m292.pep KVLSVSETPVKGIYEVWSGRQIIYTDAEGGYMFVGELINIDTRKNLTEERAADLNKIDF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a292 KVLSVSETPVKGIYEWVSGRQI IYTDAEGGYMFVGELINIDTRKNLTEERAADLNKIDF 70 80 90 100 110 120 130 140 150 160 170 180 m292 . pep ASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEKMTDVTVYSFMMPIAGLHPDA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a292 ASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEKMTDVTVYSFMMPIAGLHPDA 130 140 150 160 170 180 190 200 210 220 230 240 m292. pep ARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVAETTSLGEQFGFNGTPTLVFPNG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a292 ARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVAETTSLGEQFGFNGTPTLVFPNG 190 200 210 220 230 240 250 260 m292.pep RSQSGYSPMPQLEEIIRKNQX I I I I I I I I I I I I I I I I I I I I I a292 RSQSGYSPMPQLEEIIRKNQX 250 260 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1223>: g294.seq (partial) 1 atgcgtatta cctgtgcgcc gatgtcgctt ttgtcggcgg cagtctggtc 51 ggttcgggct gtcagaacat catcgaaccg ctttcctgcg gcgttacgac 101 gatattcggc ttttcgacct acaatttttc cgaagcctgc cggcacgcct 151 tggcatcggg tgcggcggtt caagtcgaat cggcggacgc gtggcgtgaa 201 gccgttgaaa aaaccttatc tggcgagggg ggcggaatgc agatgcaggc 251 gcgcgtggac ggctttatcg cacaacatcg cggagcgggc gcgagaatcg 301 ccgaggcggt gcgggaagcg gtatgcggac atcgggggcg atagtgatac 351 aatccgtatc cgagttttcc ggttggagca tcgtatgagt atttatgccg 401 tcgcgcacat catccacctg tattgcgcca ccgcctttgt cggcggcgtg 451 ttttttgaag tgctggtttt gtccgtcctg catacgggac gggtgtcgcg 501 cgaggcgcgg cgcgaagtgg aaaaggcaat gtcttaccgc gccgtcaggg 551 tgatgccgtt tgcggtcgga ctgctgttcg ccaggggaac tctagagtcg 601 actgcagcag catgccctc... This corresponds to the amino acid sequence <SEQ ID 1224; ORF 294.ng>: g294.pep (partial) 1 MRITCAPMSL LSAAVWSVRA VRTSΞΝRFPA ALRRYSAFRP TIFPKPAGTP 51 WHRVRRFKSΝ RRTRGVKPLK KPYLARGAEC RCRRAWTALS HΝIAERARES 101 PRRCGKRYAD IGGDSDTIRI RVFRLEHRMS IYAVAHIIHL YCATAFVGGV 151 FFEVLVLSVL HTGRVSREAR REVEKAMSYR AVRVMPFAVG LLFARGTLES 201 TAAACP.... The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1225>: m294.seq 1 ATGCGTATTA CCTGTGCGCC GATGTCGCTT TTGTCGGCGG CAGTCTGGTC 51 GATTCGGGTT GTCAGAACAT CATCGAACCG CTTTCCTGCG GCGTTCCGAC 101 GATATTCGGC TTTTCAACCT ACAATTTTTC CGAAGCCTGC CGACACGCCT 151 TGGCATCGGG TGCGGCGGTT CAAGTCGAAT CGGCGGATGC GTGGCGGGAA 201 GCCGTTGAAA AAACCTTATC GTCCGAGGGG GGGGGGATGC AGATGCAGGC 251 GCGCGTGGAC GGCTTTATCG CACAACATCG CGGAGCGGGC GCGAGAATCG 301 CCGAGGCGGT GCGGGAAGCG GTATGCGGAT ATCGGGGGCG ATAGTGATAC 351 AATCCGTATC CGAGTTTTCC GTTTGGAGCA TCGTATGAGT ATTTATGCCG 401 TCGCGCACAT CGTTCATCTG TATTGCGCTA TTGCCTTTGT CGGCGGCGTG 451 TTTTTTGAAG TGCTGGTTTT GTCCGTCCTG CATACGGGAC GGGTGTCGCG 501 CGAGGCGCGG CGCGAAGTGG AAAAGGCAAT GTCTTACCGC GCCGTCAGGG 551 TGATGCCGTT TGTGGTCGGA CTGCTGTTCG CCAGCGGCAT CGTGATGGCG 601 GCAAACCGCT ATCTTTCTAT ATTGGGCGAA CCGTTTGCCA CTTCCTTCGG 651 TACGATGCTG ACGCTGAAAA TCCTGTTGGC GTTCAGCGTA TTGGCGCACT 701 TCGCCATCGC CGTCGTCAAA ATGGCGCGTT CCACACTGAC GGTCGGTTGG 751 TCGAAATACA TACACGCCGT CGTCTTTACC CATATGcTGC TGATTGTCTT 801 TTTGGCAAAA GCGATGTTTT ATATCAGCTG GTAA This corresponds to the amino acid sequence <SEQ ID 1226; ORF 294>: m294.pep 1 MRITCAPMSL LSAAVWΞIRV VRTSSNRFPA AFRRYSAFQP TIFPKPADTP 51 WHRVRRFKSN RRMRGGKPLK KPYRPRGGGC RCRRAWTALΞ HNIAERARES 101 PRRCGKRYAD IGGDΞDTIRI RVFRLEHRMS IYAVAHIVHL YCAIAFVGGV 151 FFEVLVLSVL HTGRVSREAR REVEKAMSYR AVRVMPFVVG LLFASGIVMA 201 ANRYLSILGE PFATSFGTML TLKILLAFSV LAHFAIAVVK MARSTLTVGW 251 SKYIHAVVFT HMLLIVFLAK AMFYISW* g294/m294 92.3% identity in 196 aa overlap 10 20 30 40 50 60 g294.pep MRITCAPMSLLΞAAVWΞVRAVRTSSNRFPAALRRYSAFRPTIFPKPAGTPWHRVRRFKSN I I I I I I I I I I I I I I I I I : hi I I I I I I I I I I : I I I I I I : I I I I I I I I I I I I I I I I I I I I m294 MRITCAPMΞLLΞAAVWSIRVVRTSSNRFPAAFRRYSAFQPTIFPKPADTPWHRVRRFKΞN 10 20 30 40 50 60 70 80 90 100 110 120 g294. pep RRTRGVKPLKKPYLARGAECRCRRAWTALΞHNIAERAREΞPRRCGKRYADIGGDΞDTIRI II II lllllll Ih I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m294 RRMRGGKPLKKPYRPRGGGCRCRRAWTALΞHNIAERAREΞPRRCGKRYADIGGDΞDTIRI 70 80 90 100 110 120 130 140 150 160 170 180 g294.pep RVFRLEHRMΞIYAVAHIIHLYCATAFVGGVFFEVLVLΞVLHTGRVΞREARREVEKAMSYR I I I I I I I I I I I I I I I I hi I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m294 RVFRLEHRMSIYAVAHIVHLYCAIAFVGGVFFEVLVLΞVLHTGRVΞREARREVEKAMSYR 130 140 150 160 170 180 190 200 g294.pep AVRVMPFAVGLLFARGTLEΞTAAACP I I I I I I hi I I I I I I m294 AVRVMPFVVGLLFASGIVMAANRYLSILGEPFATSFGTMLTLKILLAFSVLAHFAIAVVK 190 200 210 220 230 240 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1227>: a294.seq 1 ATGCGTATTA CCTGTGCGCC GATGTCGCTT TTGTCGGCGG CAGTCTGGTC 51 GATTCGGGCT GTCAGAACAT CATCGAACCG CTTTCCTGCG GCGTTCCGAC 101 GATATTCGGC TTTTCGACCT ACAATTTTTC CGAAGCCTGC CGGCACGCCT 151 TGGCATCGGG TGCGGCGGTT CAAGTCGAAT CGGCGGACGC GTGGCGGGAA 201 GCCGTTGAAA AAAACTTATC GTCCGAGGAG GGCGGAATGC AGATGCAGGC 251 GCGCGCGGAC GGCTTTATCG CACAACATCG CGGAGCGGGC GCGAGAATCG 301 CCGAGGCGGT ACGGGAAGCG GTATGCGGAC ATCGGGGACG ATAGTGATAC 351 AATCCGTATC CGAGTTTTCC GGTTGGAGTA CCGTATGAGT ATTTATGCCG 401 TCGCGCACAT CGTCCACCTG TATTGCGCCA TCGCCTTTGT CGGCGGCGTG 451 TTTTTTGAAG TGCTGGTTTT GTCCGTCCTG CATACGGGAC GGGTGTCGTG 501 CGAGGCGCGG CGCGAAGTGG AAAAGGCAAT GTCTTACCGC GCCGTCAGGG 551 TGATGCCGTT TGTGGTCGGA CTGCTGTTCG CCAGCGGCAT CGTGATGGCG 601 GCAAACCGCT ATCTTTCTAT ATTGGGCGAA CCGTTTGCCA CTTCCTTCGG 651 TACGATGCTG ACGCTGAAAA TCCTGTTGGC GTTCAGCGTG TTGGCGCACT 701 TCGCCATCGC CGTCGTCAAA ATGGCGCGTT CCACACTGAC CGTCGGCTGG 751 TCGAAATACA TACACACCGT CGTCTTTACC CATATGCTGC TGATTGTCTT 801 TTTGGCAAAA GCGATGTTTT ATATCAGCTG GTAA This corresponds to the amino acid sequence <SEQ ID 1228; ORF 294.a>: a294.pep 1 MRITCAPMSL LSAAVWSIRA VRTSSNRFPA AFRRYSAFRP TIFPKPAGTP 51 WHRVRRFKSN RRTRGGKPLK KTYRPRRAEC RCRRARTALS HNIAERARES 101 PRRYGKRYAD IGDDSDTIRI RVFRLEYRMS IYAVAHIVHL YCAIAFVGGV 151 FFEVLVLSVL HTGRVSCEAR REVEKAMSYR AVRVMPFVVG LLFASGIVMA 201 ANRYLSILGE PFATSFGTML TLKILLAFSV LAHFAIAVVK MARSTLTVGW 251 SKYIHTWFT HMLLIVFLAK AMFYISW* m294/a294 94.9% identity in 277 aa overlap 10 20 30 40 50 60 m294 . pep MRITCAPMΞLLSAAVWSIRWRTSSNRFPAAFRRYSAFQPTIFPKPADTPWHRVRRFKSN I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I a294 MRITCAPMSLLSAAVWSIRAVRTSSNRFPAAFRRYSAFRPTIFPKPAGTPWHRVRRFKSN 10 20 30 40 50 60 70 80 90 100 110 120 m294.pep RRMRGGKPLKKPYRPRGGGCRCRRAWTALSHNIAERARESPRRCGKRYADIGGDSDTIRI II llllllll llll : llllll lllllllllllllllll llllllll lllllll a294 RRTRGGKPLKKTYRPRRAECRCRRARTALSHNIAERARESPRRYGKRYADIGDDSDTIRI 70 80 90 100 110 120 130 140 150 160 170 180 m294.pep RVFRLEHRMSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSREARREVEKAMSYR I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a294 RVFRLEYRMS I YAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSCEARREVEKAMSYR 130 140 150 160 170 180 190 200 210 220 230 240 m294 . pep AVRVMPFWGLLFASGIVMAANRYLSILGEPFATSFGTMLTLKILLAFSVLAHFAIAWK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a294 AVRVMPFVVGLLFASGIVMAANRYLSILGEPFATSFGTMLTLKILLAFSVLAHFAIAWK 190 200 210 220 230 240 250 260 270 m294.pep MARSTLTVGWSKYIHAWFTHMLLIVFLAKAMFYISWX I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I a294 MARSTLTVGWSKYIHTWFTHMLLIVFLAKAMFYISWX 250 260 270 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1229>: g295.seq 1 atgctcggga tggcgcggca cgacggccag cagggcatcg ccgcgatatt 51 gttgccacgc cgccagcagt ttttccgcct cgtcttcgcc ccgataaacg 101 cgcgtgctgc cgcacacggc aaccggccgg cctccgatgc gtttttcaaa 151 ctgccccgcc agcgttttca tgtcttcaga cggcatcagg tcgtatttgg 201 tattgccgca cacctgcacg gatgccgcgc ccaatttcgc caaccgcgcc 251 gcatccgcct ccgtctgcgc cagacagccc gtcagcgaag cggctgcggg 301 acggatcagg cggcggactt tcagataacc gttcagcgat ttttccgaca 351 gccgcgcatt cgccaaaaac agcggcacac ccgctcgccg gcattccttc 401 atcagattgg gccagatttc ggtttccatc aaaatgccga acatcgggcg 451 gtgttcgcgc aaaaactgcc gtacccacgt ttttttgtca tacggaagat 501 agcggcattg cgcatcggga aacagaactt gcgcggtttc ccgtcccgtc 551 ggggtcatct gcgtcatcag cagcggcgca tcgggaaaac gccgccgcaa 601 ctcgcgtatc aagggctggg cggcacgcgt ttctccgacc gaaacggcgt 651 gtatccaaac cgcgccggta acgggattcg gatgcggctt gccgaaacgc 701 tcgtccctat gcgcccggta tgccggggca cttccggagc gtttgtccaa 751 ataacgccgt atccatatcg gcgcaagcag ccacaataca tcataaagcc 801 attggaacat ctttctattt cctgcaaaac aaatgccgtc cgaacggttc 851 ggacggcatt tcggcaacgg aatcaaatat cgtag This corresponds to the amino acid sequence <SEQ ID 1230; ORF 295.ng>: g295.pep 1 MLGMARHDGQ QGIAAILLPR RQQFFRLVFA PINARAAAHG NRPASDAFFK 51 LPRQRFHVFR RHQVVFGIAA HLHGCRAQFR QPRRIRLRLR QTARQRSGCG 101 TDQAADFQIT VQRFFRQPRI RQKQRHTRSP AFLHQIGPDF GFHQNAEHRA 151 VFAQKLPYPR FFVIRKIAAL RIGKQNLRGF PΞRRGHLRHQ QRRIGKTPPQ 201 LAYQGLGGTR FSDRNGVYPN RAGNGIRMRL AETLVPMRPV CRGTΞGAFVQ 251 ITPYPYRRKQ PQYIIKPLEH LSISCKTNAV RTVRTAFRQR NQIΞ* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1231>: m295. seq 1 ATGCTCGGGA TGGCGCGGCA CGACGACCAG CAGCGCATCG CCGCGATATT 51 GTTGCCACGC CGCCAGCAGT TTTTCCGCCT CGTCTTCACC CCGATAAACG 101 CGCGTGCTGC CGCACACGGC AACCGGCCGG CCTCCGATGC GTTTTTCAAA 151 CTGCCCCGCC AGCGTTTTCA TCTGTTCCGA CGGTATGATG TCGTATTTGG 201 TATTGCCGCA CACCTGCACG GATGCCGCGC CCAATTTCGC CAACCGCGCC 251 GCATCCGCCT CTGTCTGCGC CAGACACCCC GTCAGCGAAG CGGCGGCAGG 301 ACGGATCAGG CGGCGGACTT TCAGATAACC GTTCAACGAT TTTTCCGACA 351 GCCGCGCATT CGCCAAAAAC AGCGGCACAC CCGCGCGCCG GCATTCCCTC 401 ATCAGGTTGG GCCAGATTTC GGTTTCCATC AAAATGCCGA ACATCGGGCG 451 GTGTTCGCGC AAAAACTGCC GTACCCACGT TTTTTTGTCA TACGGAAGAT 501 AGCGGCATTG CGCATCGGGA AACAGAACTT GCGCGGTTTC CCGCCCCGTC 551 GGGGTCATCT GCGTCATCAG CAGCGGCGCA TCGGGAAAAC GCCGCCGCAA 601 CTCGCGTATC AAGGACTGGG CGGCACGCGT TTCTCCGACC GAAACGGCGT 651 GTATCCAAAC CGCGCCGGTA ACGGGATTCG GATACGGCTT GCCGAAACGC 701 TCGTCCCGAT GCGCCCGATA TGCCGGGGCA CTTCCGGAGC GTTTGTCCAA 751 ATAACGCCGT ATCCATATCG GCGCAAGCAG CCACAATACA TCATAAAGCC 801 ATTGGAACAT CTTTCTATTT CCTGCAAAAC AAATGCCGTC TGAACGGTTC 851 AGACGGCATT TCGGCAACGG AATCAAATAT CGTAG This corresponds to the amino acid sequence <SEQ ID 1232; ORF 295>: m295.pep 1 MLGMARHDDQ QRIAAILLPR RQQFFRLVFT PINARAAAHG NRPAΞDAFFK 51 LPRQRFHLFR RYDVVFGIAA HLHGCRAQFR QPRRIRLCLR QTPRQRSGGR 101 TDQAADFQIT VQRFFRQPRI RQKQRHTRAP AFPHQVGPDF GFHQNAEHRA 151 VFAQKLPYPR FFVIRKIAAL RIGKQNLRGF PPRRGHLRHQ QRRIGKTPPQ 201 LAYQGLGGTR FSDRNGVYPN RAGNGIRIRL AETLVPMRPI CRGTΞGAFVQ 251 ITPYPYRRKQ PQYIIKPLEH LΞISCKTNAV XTVQTAFRQR NQIΞ* m295/g295 93.9% identity in 294 aa overlap 10 20 30 40 50 60 m295.pep MLGMARHDDQQRIAAILLPRRQQFFRLVFTPINARAAAHGNRPASDAFFKLPRQRFHLFR llllllll II I I I I I I I I I I I I I I I I hi I I I I I I I I I I I I I I I I I I I I I I I I I I :| I g295 MLGMARHDGQQGIAAILLPRRQQFFRLVFAPINARAAAHGNRPAΞDAFFKLPRQRFHVFR 10 20 30 40 50 60 70 80 90 100 110 120 m295.pep RYDVVFGIAAHLHGCRAQFRQPRRIRLCLRQTPRQRSGGRTDQAADFQITVQRFFRQPRI I :: I I I I I I I I I I I I I I I I I I I I I I I I llll I I I I I I I I I I I I I I I I I I I I I I I I I g295 RHQVVFGIAAHLHGCRAQFRQPRRIRLRLRQTARQRSGCGTDQAADFQITVQRFFRQPRI 70 80 90 100 110 120 130 140 150 160 170 180 m295.pep RQKQRHTRAPAFPHQVGPDFGFHQNAEHRAVFAQKLPYPRFFVIRKIAALRIGKQNLRGF I I I I I I I h I I I I h I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g295 RQKQRHTRSPAFLHQIGPDFGFHQNAEHRAVFAQKLPYPRFFVIRKIAALRIGKQNLRGF 130 140 150 160 170 180 190 200 210 220 230 240 m295.pep PPRRGHLRHQQRRIGKTPPQLAYQGLGGTRFΞDRNGVYPNRAGNGIRIRLAETLVPMRPI I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I : g295 PΞRRGHLRHQQRRIGKTPPQLAYQGLGGTRFΞDRNGVYPNRAGNGIRMRLAETLVPMRPV 190 200 210 220 230 240 250 260 270 280 290 m295.pep CRGTSGAFVQITPYPYRRKQPQYIIKPLEHLSISCKTNAVXTVQTAFRQRNQISX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I hi I I I I I I I I I g295 CRGTSGAFVQITPYPYRRKQPQYIIKPLEHLSISCKTNAVRTVRTAFRQRNQIΞ 250 260 270 280 290 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1233>: a295. seq 1 ATGCTCGGGA TGGCGCGGCA CGACGACCAG CAGGGCATCG CCGCGATATT 51 GTTGCCACGC CGCCAGCAGT TTTTCCGCCT CGTCTTCACC CCGATAAACG 101 CGCGTGCTGC CGCACACGGC AACCTGCCGG TCTCCGATGC GTTTTTCAAA 151 CTGCCCCGCC AGCGTTTTCA TCTGTTCCGA CGGCATCAGG TCGTATTTGG 201 TATTGCCGCA CACCTGCACG GATGCCGCGC CCAATTTCGC CAACCGCGCC 251 GCATCCGCCT CCGTCTGTGC CAGACAGCCC GTCAGCGAAG CGGCGGCAGG 301 ACGGATCAGG CTGCGGACTT TCAGATAACC GTTTAGCGAT TTTTCCGACA 351 GCCGCGCATT CGCCAAAAAC AGCGGCACAC CCGTGCGCCG GCATTCCTTC 401 ATCAGATTGG GCCAGATTTC GGTTTCCATC AAAATGCCGA ACATCGGGCG 451 GTGTTCGCGC AAAAACTGCC GTACCCACGT TTTTTTGTCA TACGGAAGAT 501 AGCGGCATTG TGCATCAGGA AACAGAACTT GCGCGGTTTC CCGTCCCGTC 551 GGGGTCATCT GCGTCATCAG CAGCGGCGCA TCGGGAAAAC GCTGCCGCAA 601 CTCGCGTATC AAAGGTTGGG CGGCACGCGT TTCCCCGACC GAAACGGCGT 651 GTATCCAAAC CGCGCCGGTA ACGGGATTCG GATACGGCTT GCCGAAACGC 701 TCGCCCCGAT GCGCCCGATA TGCAGGGGCA CTTCCGGAGC GTTTGTCCAA 751 ATAACGCCGT ATCCATATCG GCGCAAGCAG CCACAATACA TCATAAAGCC 801 ATTGGAACAT CTTTCTATTT CCTGCAAAAC AAATGCCGTC CGAACGGTTC 851 GGACGGCATT TCGGCAACGG AATCAAATAT CGTAG This corresponds to the amino acid sequence <SEQ ID 1234; ORF 295. a>: a295.pep 1 MLGMARHDDQ QGIAAILLPR RQQFFRLVFT PINARAAAHG NLPVSDAFFK 51 LPRQRFHLFR RHQWFGIAA HLHGCRAQFR QPRRIRLRLC QTARQRSGGR 101 TDQAADFQIT V*RFFRQPRI RQKQRHTRAP AFLHQIGPDF GFHQNAEHRA 151 VFAQKLPYPR FFVIRKIAAL CIRKQNLRGF PSRRGHLRHQ QRRIGKTLPQ 201 LAYQRLGGTR FPDRNGVYPN RAGNGIRIRL AETLAPMRPI CRGTSGAFVQ 251 ITPYPYRRKQ PQYIIKPLEH LSISCKTNAV RTVRTAFRQR NQIS* m295/a295 93.2% identity in 294 aa overlap 10 20 30 40 50 60 m295. pep MLGMARHDDQQRIAAILLPRRQQFFRLVFTPINARAAAHGNRPASDAFFKLPRQRFHLFR I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I: I I I I I I I I I I I I I I I I a295 MLGMARHDDQQGIAAILLPRRQQFFRLVFTPINARAAAHGNLPVSDAFFKLPRQRFHLFR 10 20 30 40 50 60 70 80 90 100 110 120 m295 . pep RYDWFGIAAHLHGCRAQFRQPRRIRLCLRQTPRQRSGGRTDQAADFQITVQRFFRQPRI I : : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I l l l l l l l l a295 RHQWFGIAAHLHGCRAQFRQPRRIRLRLCQTARQRSGGRTDQAADFQITVXRFFRQPRI 70 80 90 100 110 120 130 140 150 160 170 180 m295 . pep RQKQRHTRAPAFPHQVGPDFGFHQNAEHRAVFAQKLPYPRFFVIRKIAALRIGKQNLRGF I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I l l l l l l l a295 RQKQRHTRAPAFLHQIGPDFGFHQNAEHRAVFAQKLPYPRFFVIRKIAALCIRKQNLRGF 130 140 150 160 170 180 190 200 210 220 230 240 m295. pep PPRRGHLRHQQRRIGKTPPQLAYQGLGGTRFSDRNGVYPNRAGNGIRIRLAETLVPMRPI I lllllllllllllll llllll llllll I I I I I I I I I I I I I I I I I I I I I I: I I I I I a295 PSRRGHLRHQQRRIGKTLPQLAYQRLGGTRFPDRNGVYPNRAGNGIRIRLAETLAPMRPI 190 200 210 220 230 240 250 260 270 280 290 m295.pep CRGTSGAFVQITPYPYRRKQPQYIIKPLEHLSISCKTNAVXTVQTAFRQRNQISX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I a295 CRGTSGAFVQITPYPYRRKQPQYIIKPLEHLSISCKTNAVRTVRTAFRQRNQISX 250 260 270 280 290 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 1235>: g297.seq 1 ATGGCTGTCT TCCCACTTTC GGCAAAACAT CGGAAATACG CGCTGCGCGC 51 GCTTGCCGTT TCGATTATTC TGGTGtcgGC GGCATACATT GCttcgacag 101 aggggaccga gcgcgtcaga ccgcAGCGCG TggaacaaAA ACTGCCGCCG 151 CTGTCtTGGg gcggcaacgg CGTtcagacg gcaTATTGGG TGCAGGAGGC 201 GGTGCagccg ggggactcgC TGGCGGACGT GCTGGCGCGT TCGGGTATGG 251 CGCGGGacga gattgCCcga ATcacGGAAA aataTggcgG CGAAGCCGAT 301 TTGCGgcatt tGCGTGCCGA CCAGTCGGTT CATGTTTTGG TCGGCGGCGA 351 CGGCAGTGCG CGCGAAGTGC AGTTTTttaC CGACGAAGAC GGCGAGCGCA 401 aTctGGTCGC TTTGGAAAAA AAAGGCGGCA TATGGCGGCG GTCGGCTTCT 451 GATGCGGATA TGAAGGTTTT GCCGACACTG CGTTCGGTCG TGGTCAAAAC 501 GTCGGCGCGC GGTTCGCTGG CGCGGGCGGA AGTGCCCGTC GAAATCCGCG 551 AATCCTTAAG CGGGATTTTT GCCGGCCGCT TCAGCCTTGA CGGTTTGAAG 601 GAAGGCGATG CCGTGCGCCT GCTTTACGAC AGCCTGTATT TCCACGGGCA 651 GCAGGTGGCG GCGGGCGATA TTTTGGCGGC GGAAGTTGTC AAGGGCGGCA 701 CAACCCATCA GGCGTTCTAT TACCGTTCGG ACAAGGAAGG CGGAGGGGGC 751 GGCAATTATT ACGATGAAGA CGGCAGGGTG TTGCAGGAAA AAGGCGGCTT 801 CAACATCgaG CCGCTGGTCT ATACGCGCAT TTCTTCGCCG TTCGGCTACC 851 GTATGCACCC CATCCTGCAC ACATGGCGGC TGCACACGGG CATCGATTAT 901 GCCGCACCGC AGGGAACGCC GGTCAGGGCT TCCGCCGACG GCGTGATTAC 951 CTTTAAAGGC CGGAAGGGCG GATACGGCAA CGCGGTGATG ATACGCCACG 1001 CCAACGGTGT GGAAACGCTG TACGCGCACT TGAGCGCGTT TTCGCAGGCA 1051 CAAGGCAATG TGCGCGGCGG CGAGGTCATC GGTTTTGTCG GTTCGACAGG 1101 GCGTTCGACC GGGCCGCACC TGCATTACGA GGCGCGCATC AACGGGCAGC 1151 CCGTCAATCC TGTTTCGGTC GCATTGCCGA CACCCGAATT GACGCAGGCG 1201 GACAAGGCGG CGTTTGCCGC GCAGAAACAG AAGGCGGACG CGCTGCTTGC 1251 GCGCTTGCGC GGCATACCGG TTACCGTGTC GCAATCGGAT TGA This corresponds to the amino acid sequence <SEQ ID 1236; ORF 297.ng>: g297.pep 1 MAVFPLΞAKH RKYALRALAV ΞIILVSAAYI ASTEGTERVR PQRVEQKLPP 51 LSWGGNGVQT AYWVQEAVQP GDSLADVLAR ΞGMARDEIAR ITEKYGGEAD 101 LRHLRADQSV HVLVGGDGΞA REVQFFTDED GERNLVALEK KGGIWRRSAS 151 DADMKVLPTL RSVVVKTSAR GSLARAEVPV EIREΞLSGIF AGRFΞLDGLK 201 EGDAVRLLYD SLYFHGQQVA AGDILAAEVV KGGTTHQAFY YRSDKEGGGG 251 GNYYDEDGRV LQEKGGFNIE PLVYTRISΞP FGYRMHPILH TWRLHTGIDY 301 AAPQGTPVRA ΞADGVITFKG RKGGYGNAVM IRHANGVETL YAHLΞAFSQA 351 QGNVRGGEVI GFVGSTGRΞT GPHLHYEARI NGQPVNPVSV ALPTPELTQA 401 DKAAFAAQKQ KADALLARLR GIPVTVSQSD * The following partial DNA sequence was identified in N meningitidis <SEQ ID 1237>: m297.seq 1 ATGGCTGTCT TCCCACTTTC GGCAAAACAT CGGAAATACG CGCTGCGTGC 51 GCTTGCCGTT TCGATTATTT TGGTGTCGGC GGCATACATT GCTTCGACAG 101 AGAGGACGGA GCGCGTCAGA CCGCAGCGCG TGGAACAAAA TCTGCCGCCG 151 CTGTCTTGGG GCGGCAGCGG CGTTCAGACG GCATATTGGG TGCAGGAGGC 201 GGTGCAGCCG GGCGACTCGC TGGCGGACGT GCTGGCGCGT TCGGGTATGG 251 CGCGGGACGA GATTGCCCGA ATCACGGAAA AATATGGCGG CGAAGCCGAT 301 TTGCGGCATT TGCGTGCCGA CCAGTCGGTT CATGTTTTGG TCGGCGGCGA 351 CGGCGGCGCG CGCGAAGTGC AGTTTTTTAC CGACGAAGAC GGCGAGCGCA 401 ATCTGGTCGC TTTGGAAAAG AAAGGCGGCA TATGGCGGCG GTCGGCTTCT 451 GAGGCGGATA TGAAGGTTTT GCCGACGCTG CGTTCGGTCG TGGTCAAAAC 501 GTCGGCGCGC GGTTCGCTGG CGCGGGCGGA AGTGCCCGTC GAAATCCGCG 551 AATCCTTAAG CGGGATTTTC GCCGGCCGCT TCAGCCTTGA CGGTTTGAAG 601 GAAGGCGATG CCGTGCGCCT GATGTACGAC AGCCTGTATT TCCACGGGCA 651 GCAGGTGGCG GCGGGCGATA TTTTGGCGGC TGAAGTCGTT AAGGGCGGCA 701 CAAGGCATCA GGCGTTCTAT TACCGTTCGG ACAAGGAAGG CGGAGGGGGC 751 GGCAATTATT ATGATGAAGA CGGCAAGGTG TTGCAGGAAA AAGGCGGCTT 801 CAACATCGAG CCGCTGGTCT ATACGCGCAT TTCTTCGCCG TTCGGCTACC 851 GTATGCACCC CATCCTGCAC ACATGGCGGC TGCACACGGG CATCGATTAT 901 GCCGCACCGC AGGGAACGCC GGTCAGGGCT TCCGCCGACG GCGTGATTAC 951 CTTTAAAGGC CGGAAGGGCG GATACGGCAA CGCGGTGATG ATACGCCACG 1001 CCAACGGTGT GGAAACGCTG TACGCGCACT TGAGCGCGTT TTCGCAGGCG 1051 GAAGGCAATG TGCGCGGCGG CGAGGTCATC GGTTTTGTCG GTTCGACCGG 1101 GCGTTCGACC GGGCCGCACC TGCATTACGA GGCGCGCATC AACGGGCAGC 1151 CCGTCAATCC TGTTTCGGTC GCATTGCCGA CACCGGAATT GACGCAGGCG 1201 GACAAGGCGG CGTTTGCCGC GCAGAAACAG AAGGCGGACG CGCTGCTTGC 1251 GCGCTTGCGC GGCATACCGG TTACCGTGTC GCAATCGGAT TGA This corresponds to the amino acid sequence <SEQ ID 1238; ORF 297>: m297.pep 1 MAVFPLΞAKH RKYALRALAV ΞIILVSAAYI ASTERTERVR PQRVEQΝLPP 51 LΞWGGSGVQT AYWVQEAVQP GDΞLADVLAR SGMARDEIAR ITEKYGGEAD 101 LRHLRADQSV HVLVGGDGGA REVQFFTDED GERNLVALEK KGGIWRRSAS 151 EADMKVLPTL RSVVVKTSAR GSLARAEVPV EIRESLΞGIF AGRFΞLDGLK 201 EGDAVRLMYD SLYFHGQQVA AGDILAAEVV KGGTRHQAFY YRSDKEGGGG 251 GNYYDEDGKV LQEKGGFNIE PLVYTRISΞP FGYRMHPILH TWRLHTGIDY 301 AAPQGTPVRA ΞADGVITFKG RKGGYGNAVM IRHANGVETL YAHLΞAFΞQA 351 EGNVRGGEVI GFVGSTGRST GPHLHYEARI NGQPVNPVSV ALPTPELTQA 401 DKAAFAAQKQ KADALLARLR GIPVTVSQΞD * 297/g297 97.9% identity in 430 aa overlap 10 20 30 40 50 60 m297.pep MAVFPLΞAKHRKYALRALAVSIILVΞAAYIASTERTERVRPQRVEQNLPPLSWGGSGVQT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I hi I I I I I I h I I I I g297 MAVFPLΞAKHRKYALRALAVS11LVSAAYIASTEGTERVRPQRVEQKLPPLΞWGGNGVQT 10 20 30 40 50 60 70 80 90 100 110 120 m297. pep AYWVQEAVQPGDΞLADVLARSGMARDEIARITEKYGGEADLRHLRADQSVHVLVGGDGGA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I h I g297 AYWVQEAVQPGDΞLADVLARSGMARDEIARITEKYGGEADLRHLRADQSVHVLVGGDGSA 70 80 90 100 110 120 130 140 150 160 170 180 m297.pep REVQFFTDEDGERNLVALEKKGGIWRRΞAΞEADMKVLPTLRΞVVVKTΞARGΞLARAEVPV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g297 REVQFFTDEDGERNLVALEKKGGIWRRSASDADMKVLPTLRSVVVKTSARGSLARAEVPV 130 140 150 160 170 180 190 200 210 220 230 240 m297.pep EIRESLSGIFAGRFSLDGLKEGDAVRLMYDSLYFHGQQVAAGDILAAEVVKGGTRHQAFY I I I I I I I I I I I I I I I I I I I I I I I I I I hi I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g297 EIRESLSGIFAGRFSLDGLKEGDAVRLLYDSLYFHGQQVAAGDILAAEVVKGGTTHQAFY 190 200 210 220 230 240 250 260 270 280 290 300 m297.pep YRΞDKEGGGGGNYYDEDGKVLQEKGGFNIEPLVYTRIΞΞPFGYRMHPILHTWRLHTGIDY I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g297 YRΞDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTRIΞΞPFGYRMHPILHTWRLHTGIDY 250 260 270 280 290 300 310 320 330 340 350 360 m297.pep AAPQGTPVRASADGVITFKGRKGGYGNAVMIRHANGVETLYAHLSAFSQAEGNVRGGEVI I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I hi I I I I I I I I g297 AAPQGTPVRASADGVITFKGRKGGYGNAVMIRHANGVETLYAHLΞAFSQAQGNVRGGEVI 310 320 330 340 350 360 370 380 390 400 410 420 m297.pep GFVGSTGRSTGPHLHYEARINGQPVNPVSVALPTPELTQADKAAFAAQKQKADALLARLR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g297 GFVGSTGRΞTGPHLHYEARINGQPVNPVSVALPTPELTQADKAAFAAQKQKADALLARLR 370 380 390 400 410 420 430 m297.pep GIPVTVSQΞDX I I I I I I I I I I I g297 GIPVTVΞQΞDX 430 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1239>: a297.seq 1 ATGGCTGTCT TCCCACTTTC GGCAAAACAT CGGAAATACG CGCTGCGCGC 51 GCTTGCCGTT TCGATTATTT TGGTGTCGGC GGCATACATT GCTTCGACAG 101 AGAGGACGGA GCGCGTCAGA CCGCAGCGCG TGGAACAAAA ACTGCCGCCG 151 CTGTCTTGGG GCGGCAGCGG TGTTCAGACG GCATATTGGG TGCAGGAGGC 201 GGTGCAGCCA GGCGACTCGC TGGCGGACGT GCTGGCGCGT TCGGGTATGG 251 CGCGGGACGA AATTGCCCGA ATAACGGAAA AATATGGCGG CGAAGCCGAT 301 TTGCGGCATT TGCGTGCCGA CCAGTCGGTT CATGTTTTGG TCGGCGGCGA 351 CGGCGGCGCG CGCGAAGTGC AGTTTTTTAC CGACGAAGAC GGCGAGCGCA 401 ATCTGGTCGC TTTGGAAAAA AAAGGCGGCA TATGGCGGCG GTCGGCTTCT 451 GAGGCGGATA TGAAGGTTTT GCCGACGCTG CGTTCGGTCG TGGTCAAAAC 501 GTCGGCGCGC GGTTCGCTGG CGCGGGCGGA AGTGCCCGTC GAAATTCGCG 551 AATCCTTAAG CGGGATTTTC GCCGGCCGCT TCAGCCTTGA TGGTTTGAAG 601 GAAGGCGATG CCGTGCGCCT GATTTACGAC AGCCTGTATT TCCACGGGCA 651 GCAGGTGGCG GCGGGCGATA TTCTGGCGGC GGAAGTCGTT AAGGGCGGCA 701 CAAGGCATCA GGCGTTCTAT TACCGTTCGG ACAAGGAAGG AGGAGGGGGC 751 GGCAATTATT ACGATGAAGA CGGCAGGGTG TTGCAGGAAA AAGGCGGCTT 801 CAACATCGAG CCACTGGTCT ATACGCGCAT TTCTTCGCCG TTCGGCTACC 851 GTATGCACCC CATCCTGCAC ACTTGGCGGC TGCACACGGG CATCGATTAT 901 GCCGCACCGC AGGGAACGCC GGTCAGGGCT TCCGCCGACG GCGTGATTAC 951 CTTTAAAGGC CGGAAGGGTG GCTACGGCAA CGCGGTGATG ATACGCCACG 1001 CCAACGGTGT GGAAACGCTG TATGCGCACT TGAGCGCGTT TTCTCAGGCA 1051 GAAGGCAATG TGCGCGGCGG CGAGGTCATC GGTTTTGTCG GTTCGACCGG 1101 GCGTTCGACG GGGCCGCACC TGCATTACGA GGCGCGCATC AATGGGCAGC 1151 CCGTCAATCC TGTTTCGGTC GCATTGCCGA CACCCGAATT GACGCAGGCG 1201 GACAAGGCGG CGTTTGCCGC GCAGAAACAG AAGGCGGACG CGCTGCTTGC 1251 GCGCTTGCGC GGCATACCGG TTACCGTGTC GCAATCGGAT TGA This corresponds to the amino acid sequence <SEQ ID 1240; ORF 297. a>: a297.pep 1 MAVFPLSAKH RKYALRALAV SIILVSAAYI ASTERTERVR PQRVEQKLPP 51 LSWGGSGVQT AYWVQEAVQP GDSLADVLAR SGMARDEIAR ITEKYGGEAD 101 LRHLRADQSV HVLVGGDGGA REVQFFTDED GERNLVALEK KGGIWRRSAS 151 EADMKVLPTL RSVVVKTSAR GSLARAEVPV EIRESLSGIF AGRFSLDGLK 201 EGDAVRLIYD SLYFHGQQVA AGDILAAEVV KGGTRHQAFY YRSDKEGGGG 251 GNYYDEDGRV LQEKGGFNIE PLVYTRISSP FGYRMHPILH TWRLHTGIDY 301 AAPQGTPVRA SADGVITFKG RKGGYGNAVM IRHANGVETL YAHLSAFSQA 351 EGNVRGGEVI GFVGSTGRST GPHLHYEARI NGQPVNPVSV ALPTPELTQA 401 DKAAFAAQKQ KADALLARLR GIPVTVSQSD * m297/a297 99.3% identity in 430 aa overlap 10 20 30 40 50 60 m297. pep MAVFPLSAKHRKYALRALAVSIILVSAAYIASTERTERVRPQRVEQNLPPLSWGGSGVQT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I a297 MAVFPLSAKHRKYALRALAVSIILVSAAYIASTERTERVRPQRVEQKLPPLSWGGSGVQT 10 20 30 40 50 60 70 80 90 100 110 120 m297.pep AYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLRHLRADQSVHVLVGGDGGA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a297 AYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLRHLRADQSVHVLVGGDGGA 70 80 90 100 110 120 130 140 150 160 170 180 m297. pep REVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTLRSVWKTSARGSLARAEVPV I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a297 REVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTLRSVWKTSARGSLARAEVPV 130 140 150 160 170 180 190 200 210 220 230 240 m297.pep EIRESLSGIFAGRFSLDGLKEGDAVRLMYDSLYFHGQQVAAGDILAAEWKGGTRHQAFY I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a297 EIRESLSGIFAGRFSLDGLKEGDAVRLIYDSLYFHGQQVAAGDILAAEWKGGTRHQAFY 190 200 210 220 230 240 250 260 270 280 290 300 m297.pep YRSDKEGGGGGNYYDEDGKVLQEKGGFNIEPLVYTRISSPFGYRMHPILHTWRLHTGIDY I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a297 YRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTRISSPFGYRMHPILHTWRLHTGIDY 250 260 270 280 290 300 310 320 330 340 350 360 m297. pep AAPQGTPVRASADGVITFKGRKGGYGNAVMIRHANGVETLYAHLSAFSQAEGNVRGGEVI I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a297 AAPQGTPVRASADGVITFKGRKGGYGNAVMIRHANGVETLYAHLSAFSQAEGNVRGGEVI 310 320 330 340 350 360 370 380 390 400 410 420 m297. pep GFVGSTGRSTGPHLHYEARINGQPVNPVSVALPTPELTQADKAAFAAQKQKADALLARLR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a297 GFVGSTGRSTGPHLHYEARINGQPVNPVSVALPTPELTQADKAAFAAQKQKADALLARLR 370 380 390 400 410 420 430 m297.pep GIPVTVSQSDX I I I I I I I I I I I a297 GIPVTVSQSDX 430 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1241>: g298.seq 1 ATGAAAAACT TTCTTTCCCT TTTCGCCTCC ATACTGATGT CTGCCCTGAT 51 TGCCGTGTGG TTCAGCCAAA ACCCCATCAA CGCCTACTGG CAGCAGACCT 101 ACCACCGCAA CAGCCCGCTC GAACCGCTTG CCGCCTACGG ATGGTGGCGG 151 AGCGGAGCGG CGTTGCAAGA AAACGCCTAC GCCCTTTCAG ACGGCATCAA 201 AACCTTCCTG TCCGGCGAAA cgccccccac ggCTCAAGAC GGCGGTTCGG 251 CAGATATGCC GCCTGAAGCC GCCGCATCCG AAGCCGCCCC GCCGGCCGGC 301 GGAACAGAAT GGAAACAAGG CACCGAAGCC GCCGCCGTCC GCAGCGGCGA 351 CAAAGTCTTT TTCGCCGGAG ATTCGCTGAT GCAGGGCGTT GCGCCTTTCG 401 TGCAAAAAAG CCTGAAACAG CAATACGGCA TCGAATCCGC CAACCTCAGC 451 AAACAAAGCA CGGGGCTTTC CTATCCCTCA TTCTTCGACT GGCCGAAAAC 501 GATTGAAGAA ACCTTGAAAA AACATCCCGA AATCAGCGTA CTCGCCGTCT 551 TCCTCGGCCC GAACGACCCG TGGGATTTCC CCGTCGGCAA ACGCTACCTC 601 AAATTCGCTT CCGACGAATG GGCGCAAGAA TACCTGAAAC GCGTCGACCG 651 CATCCTTGAA GCCGCACACA CGCACCGCGT CCAAGTCGTC TGGCTCGGCA 701 TCCCCTACAT GAAAAAAGTC AAGCTCGACG GTCAGATGCG CTACCTCGAC 751 AAACTGCTTT CGGAACACTT GAAAGGCAAA ATCATCCTGA TTCCCACCGC 801 GCAAACACTG AGCGGCGGGA AAGgccGCTA CACCGATTCC GTCAACGTCA 851 ACGGCAAACC CGTCCGCTAC CGCAGTAAGG ACGGCATACA CTTTACCGCC 901 GAAGGACAAA AACTGCTGGC GGAAAAAATA ATGGAAAAAA TCGTTTTTGA 951 ACCGAGTACG CAACCATCAA GTACACAGCC ATGA This corresponds to the amino acid sequence <SEQ ID 1242; ORF 298.ng>: g298.pep 1 MKΝFLSLFAS ILMSALIAVW FSQΝPIΝAYW QQTYHRΝSPL EPLAAYGWWR 51 SGAALQEΝAY ALSDGIKTFL SGETPPTAQD GGSADMPPEA AASEAAPPAG 101 GTEWKQGTEA AAVRSGDKVF FAGDΞLMQGV APFVQKSLKQ QYGIESAΝLS 151 KQSTGLSYPΞ FFDWPKTIEE TLKKHPEISV LAVFLGPΝDP WDFPVGKRYL 201 KFASDEWAQE YLKRVDRILE AAHTHRVQW WLGIPYMKKV KLDGQMRYLD 251 KLLΞEHLKGK IILIPTAQTL SGGKGRYTDS VΝVΝGKPVRY RSKDGIHFTA 301 EGQKLLAEKI MEKIVFEPST QPSSTQP* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1243>: m298.seq 1 ATGAAAAACT TTCTTTCCCT TTTCTCCTCC ATACTGATGT CTGCCCTGAT 51 TGCCGTGTGG TTCAGCCAAA ACCCCATCAA CGCCTACTGG CAGCAGACCT 101 ACCACCGCAA CAGCCCGCTC GAACCGCTTG CCGCCTACGG ATGGTGGCGG 151 AGCGGTGCGG CGTTGCAAGA AAACGCCTAC GCCCTTTCAG ACGGCATCAA 201 AGCCTTCCTG TCCGGCGAAA CGCCGCCGAC GGCTCAAGAC GGCGGTTCGG 251 CAGATATGCC GTCTGAAGCC GCCGCATCCG AAGCCGTCCC TCAAACCGGT 301 GAAACAGAAT GGAAACAAGA CACCGAAGCC GCCGCCGTCC GCAGCGGCGA 351 CAAAGTCTTT TTTGTCGGCG ACTCGCTGAT GCAGGGCGTT GCCCCCTTCG 401 TGCAAAAAAG CCTGAAACAG CAATACGGCA TCGAATCCGT CAACCTCAGC 451 AAACAAAGCA CGGGGCTGTC CTACCCCTCA TTCTTCGACT GGCCGAAAAC 501 GATTGAAGAA ACCCTGCAAA AACATCCCGA AATCAGCGTA CTCGCCGTCT 551 TCCTCGGACC GAACGACCCG TGGGATTTCC CCGTCGGCAA ACTCTATCTC 601 AAATTCGCTT CCGACGAATG GGCGCAAGAA TACCTGAAAC GTGTCGACCG 651 CATCCTTGAA GCCGCACACA CGCACCGCGT CCAAGTCGTC TGGCTCGGCA 701 TCCCCTACAT GAAAAAAGCC AAGCTCGACG GACAGATGCG CTACCTAGAC 751 AAACTGCTTT CGGAACATTT GAAAGGCAAA ATCATCCTGA TTCCCACCAC 801 GCACACCCTG AGCGGCGGGA AAGACCGCTA CACCGACTCC GTCAACGTCA 851 ACGGCAAACC CGTCCGCTAC CGCAGCAAGG ACGGCATACA CTTTACCGCC 901 GAAGGACAAA AACTGCTGGC GGCAAAAATA ATGGAAAAAA TCGTTTTTGA 951 ACCAAGTACG CAACCATCAA GTACACAGCC ATGA This corresponds to the amino acid sequence <SEQ ID 1244; ORF 298>: m298.pep 1 MKNFLSLFSS ILMSALIAVW FSQNPINAYW QQTYHRNSPL EPLAAYGWWR 51 SGAALQENAY ALSDGIKAFL SGETPPTAQD GGSADMPSEA AASEAVPQTG 101 ETEWKQDTEA AAVRSGDKVF FVGDSLMQGV APFVQKSLKQ QYGIESVNLΞ 151 KQΞTGLSYPS FFDWPKTIEE TLQKHPEISV LAVFLGPNDP WDFPVGKLYL 201 KFASDEWAQE YLKRVDRILE AAHTHRVQW WLGIPYMKKA KLDGQMRYLD 251 KLLSEHLKGK IILIPTTHTL SGGKDRYTDS VNVNGKPVRY RSKDGIHFTA 301 EGQKLLAAKI MEKIVFEPST QPSSTQP* m298/g298 94.8% identity in 327 aa overlap 10 20 30 40 50 60 m298.pep MKNFLSLFSSILMSALIAVWFΞQNPINAYWQQTYHRNSPLEPLAAYGWWRSGAALQENAY E 11 E 1111 H I ! 111 i i 11 i ! 11111 ! 111111 E M E 111111111111 i 1111 ! 11111 g298 MKNFLSLFASILMΞALIAVWFSQNPINAYWQQTYHRNSPLEPLAAYGWWRΞGAALQENAY 10 20 30 40 50 60 70 80 90 100 110 120 m298.pep ALΞDGIKAFLSGETPPTAQDGGΞADMPSEAAASEAVPQTGETEWKQDTEAAAVRSGDKVF 11 i 11 i j: 1111111111 ! 11111 i 11 ! 1111 M : I = 1 MM! g298 ALSDGIKTFLSGETPPTAQDGGSADMPPEAAASEAAPPAGGTEWKQGTEAAAVRSGDKVF 70 80 90 100 110 120 130 140 150 160 170 180 m298.pep FVGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLΞYPSFFDWPKTIEETLQKHPEISV I H II 11111111 i 11111111 II I i U 1111111 ! I II 111 i I M II 1111 : 111 II I ! g298 FAGDSLMQGVAPFVQKSLKQQYGIESANLSKQSTGLSYPSFFDWPKTIEETLKKHPEISV 130 140 150 160 170 180 190 200 210 220 230 240 m298.pep LAVFLGPNDPWDFPVGKLYLKFASDEWAQEYLKRVDRILEAAHTHRVQWWLGIPYMKKA g298 AVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRILEAAHTHRVQWWLGIPYMKKV 190 200 210 220 230 240 250 260 270 280 290 300 m298.pep KLDGQMRYLDKLLSEHLKGKIILIPTTHTLSGGKDRYTDSVNVNGKPVRYRSKDGIHFTA g298 KLDGQMRYLDKLLSEHLKGKIILIPTAQTLSGGKGRYTDΞVNVNGKPVRYRSKDGIHFTA 250 260 270 280 290 300 310 320 m298.pep EGQKLLAAKIMEKIVFEPSTQPSSTQPX g298 EGQKLLAEKIMEKIVFEPSTQPSSTQPX 310 320 The following partial DNA sequence was identified inN meningitidis <SEQ ID 1245>: a298.seq 1 ATGAAAAACT TTCTTTCCCT TTTCGCCTCC ATACTGATGT CTGCCCTGAT 51 TGCCGTGTGG TTCAGCCAAA ACCCCATCAA CGCCTACTGG CAGCAGACCT 101 ACCACCGCAA CAGCCCGCTC GAACCGCTTG CCGCCTACGG ATGGTGGCGG 151 AGCGGTGCGG CATTGCAAGA AAACGCCTAC GCCCTTTCAG ACGGCATCAA 201 AGCCTTCCTG TCCGGCGAAA CGCCGCCGAC GGCTCAAGAC GGCGGTTCGG 251 CAGATATGCC GTCTGAAGCC GCCGCACCCG AAACCGCCCC TCAAACTGGC 301 GAAACAGAAT GGAAACAAAA CACCGAAGCC GCCGCCGTCC GAACAGGGGA 351 CAAAGTCTTT TTCGCCGGCG ACTCGCTGAT GCAGGGCGTT GCACCCTTCG 401 TGCAAAAAAG CCTGAAACAG CAATACGGCA TCGAATCCGT CAACCTCAGC 451 AAACAAAGCA CGGGGCTGTC CTACCCCTCA TTCTTCGACT GGCCGAAAAC 501 GATTGAAGAA ACCCTGAAAA AACATCCCGA AATCAGCGTG CTCGCCGTCT 551 TCCTCGGTCC GAACGACCCG TGGGATTTCC CCGTTGGCAA ACGCTACCTC 601 AAATTCGCTT CCGACGAATG GGCGCAAGAA TACCTGAAAC GCGTCGACCG 651 CATCCTTGAA GCCGCACACA CGCACTACGT CCAAGTCGTC TGGCTCGGCA 701 TCCCCTACAT GAAAAAAGCC AAGCTCGACG GACAGATGCG CTACCTAGAC 751 AAACTGCTTT CGGAATATTT GAAAGGCAAA ATCATCCTGA TTCCCACCGC 801 GCACACCCTG AGCGGCGGGA AAGACCGCTA CACCGACTCC GTCAACGTCA 851 ACGGCAAACC CGTCCGCTAC CGCAGCAAGG ACGGCATACA CTTTACCGCC 901 GAAGGACAAA AACTGCTGGC GGCAAAAATA ATGGAAAAAA TCGTTTTTGA 951 ACCAAGTACG CAACCATCAA GTACACAGCC ATGA This corresponds to the amino acid sequence <SEQ ID 1246; ORF 298. a>: a298.pep 1 MKNFLSLFAS ILMSALIAVW FSQNPINAYW QQTYHRNSPL EPLAAYGWWR 51 SGAALQENAY ALSDGIKAFL SGETPPTAQD GGSADMPSEA AAPETAPQTG 101 ETEWKQNTEA AAVRTGDKVF FAGDSLMQGV APFVQKSLKQ QYGIESVNLS 151 KQSTGLSYPS FFDWPKTIEE TLKKHPEISV LAVFLGPNDP WDFPVGKRYL 201 KFASDEWAQE YLKRVDRILE AAHTHYVQW WLGIPYMKKA KLDGQMRYLD 251 KLLSEYLKGK IILIPTAHTL SGGKDRYTDS VNVNGKPVRY RSKDGIHFTA 301 EGQKLLAAKI MEKIVFEPST QPSSTQP* m298/a298 96.3% identity in 327 aa overlap 10 20 30 40 50 60 m298.pep MKNFLSLFSSILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLAAYGWWRSGAALQENAY I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I a298 MKNFLSLFASILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLAAYGWWRSGAALQENAY 10 20 30 40 50 60 70 80 90 100 110 120 m298.pep ALSDGIKAFLSGETPPTAQDGGSADMPSEAAASEAVPQTGETEWKQDTEAAAVRSGDKVF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : : I I I I I I I I I I : I I I I I I I : I I I I I a298 ALSDGIKAFLSGETPPTAQDGGSADMPSEAAAPETAPQTGETEWKQNTEAAAVRTGDKVF 70 80 90 100 110 120 130 140 150 160 170 180 m298.pep FVGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYPSFFDWPKTIEETLQKHPEISV I : I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I h I I I I I I I a298 FAGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYPSFFDWPKTIEETLKKHPEISV 130 140 150 160 170 180 190 200 210 220 230 240 m298. pep LAVFLGPNDPWDFPVGKLYLKFASDEWAQEYLKRVDRILEAAHTHRVQWWLGIPYMKKA lllllllllllllllll I I I I I I I I I I I I I I I I I I I I I I I I I I I llllllllllllll a298 LAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRILEAAHTHYVQWWLGIPYMKKA 190 200 210 220 230 240 250 260 270 280 290 300 m298. pep KLDGQMRYLDKLLSEHLKGKIILIPTTHTLSGGKDRYTDSVNVNGKPVRYRSKDGIHFTA I I I I I I I I I II II I I : I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a298 KLDGQMRYLDKLLSEYLKGKIILIPTAHTLSGGKDRYTDSVNVNGKPVRYRSKDGI-HFTA 250 260 270 280 290 300 310 320 m298.pep EGQKLLAAKIMEKIVFEPSTQPSSTQPX I I I I I I I I I I I I I I I I I I II I I I I I I I I a298 EGQKLLAAKIMEKIVFEPSTQPSSTQPX 310 320 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1247>: g299.seq 1 ATGAACCCCA AACACTTCAT CGCATTTTCC GCCCTGTTCG CCGCCACGCA 51 GGCAGAAGCC CTGCCCGTCG CCTCCGTCAG CCCCGACACC GTTACCGTTT 101 CCCCGTCCGC CCCCTACACC GATACAAACG GGCTGCTGAC CGACTACGGC 151 AACGCCGCCG CCTCGCCTTG GATGAAAAAA CTCCGATCCG TCGCACAAGG 201 CAGCGGCGAG GCCTTCCGCA TCCTGCAAAT CGGCGACTCG CATACCGCCG 251 GCGACTTCTT TACCGACGCC CTGCGCAAAC GCCTGCAAAA AACATGGGGC 301 GACGGCGGCA TAGGCTGGGT TTACCCCGCC AACGTCAAAG GGCAGCGCAT 351 GGCGGCCGTC CGTCACAGCG GCAACTGGCA AAGCTTCACC AGCAGGAACA 401 ATACCGGAGA TTTCCCGCTC GGCGGCATCC TCGCCCAAAC CGGCAGCGGC 451 GGCGGCATGA CCCTGACCGC GTCTGACGGC AAAACCGGCA AACAGCGCGT 501 TTCCCTGTTT GCCAAACCGC TGCTCGCCGA ACAAACCCTG ACCGTCAACG 551 GCAACACCGT CTCCGCCAAC GGCGGCGGCT GGCAGGTACT GGATACGGGC 601 GCGGCACTGC CCCTGGCCAT ACAGACCGAA ATGCCGTGGG ACATCGGCTT 651 CATCAACATC GAAAATCCCG CCGGCGGCAT TACCGTTTCC GCGATGGGCA 701 TCAACGGCGC ACAATTGACC CAGTGGTCGA AATGGCGTGC CGACCGTATG 751 AACGACCTTG CCCAAACCGG CGCCGATTTG GTTATCCTTT CCTACGGCAC 801 CAACGAAGCC TTCAACAACA ACATCGACAT TGCCGATACC GAACAAAAAT 851 GGCTGGATAC CGTCCGCCAA ATCCGCGACA GCCTGCCCGC CGCCGGCATC 901 CTCATCATCG GCGCGCCCGA ATCCCTGAAA AACACGCTCG GCGTATGCGG 951 CACGCGCCCC GTCCTCCTGA CCGAAGTCCA ACAGATGCAG CGGCGCGTCG 1001 CCCGTCAGGG GCAGACGATG TTTTGGTCTT GGCAAAACGC AATGGGCGGC 1051 ATATGCAGCA TGAAAAACTG GCTCAACCAA GGATGGGCCG CCAAAGACGG 1101 CGTACACTTC TCCGCCCAAG GCTACCGGCG CGCGGCGGAA ATGCTTGCCG 1151 ACAGCCTCGA AGAACTCGTC CGCGCCGCCG CAATCAGGCA ATAA This corresponds to the amino acid sequence <SEQ ID 1248; ORF 299.ng>: g299.pep 1 MNPKHFIAFS ALFAATQAEA LPVASVΞPDT VTVSPSAPYT DTNGLLTDYG 51 NAAASPWMKK LRSVAQGSGE AFRILQIGDS HTAGDFFTDA LRKRLQKTWG 101 DGGIGWVYPA NVKGQRMAAV RHSGNWQSFT SRNNTGDFPL GGILAQTGSG 151 GGMTLTASDG KTGKQRVSLF AKPLLAEQTL TVNGNTVSAN GGGWQVLDTG 201 AALPLAIQTE MPWDIGFINI ENPAGGITVS AMGINGAQLT QWSKWRADRM 251 NDLAQTGADL VILSYGTNEA FNNNIDIADT EQKWLDTVRQ IRDSLPAAGI 301 LIIGAPESLK NTLGVCGTRP VLLTEVQQMQ RRVARQGQTM FWSWQNAMGG 351 ICSMKNWLNQ GWAAKDGVHF SAQGYRRAAE MLADSLEELV RAAAIRQ* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1249>: m299.seq 1 ATGAACCCCA AACACCTCAT CGCATTTTCC GCCCTATTCG CCGCCACGCA 51 GGCAGAAGCC CTACCTGTCG CCTCCGTCAG CCTCGACACC GTTACCGTTT 101 CCCCGTCCGC CCCCTACACC GATACAAACG GGCTGCTGAC CGACTACGGC 151 AACGCCTCCG CCTCGCCTTG GATGAAAAAA CTCCAATCCG TCGCACAAGG 201 CAGCGGCGAG ACCTTCCGTA TCCTGCAAAT CGGCGACTCG CATACCGCCG 251 GCGACTTCTT TACCGACAGC CTGCGCAAAC GCCTGCAAAA AACTTGGGGC 301 GACGGCGGCA TAGGCTGGGT TTACCCCGCC AACGTCAAAG GGCAGCGCAT 351 GGCGGCCGTC CGGCACAACG GTAACTGGCA AAGCCTCACC AGCAGGAACA 401 ACACCGGAGA CTTCCCGCTC GGCGGCATCC TCGCCCACAC CGGCAGCGGC 451 GGCAGCATGA CCCTGACCGC ATCGGACGGC ATAGCAAGCA AGCAGCGCGT 501 TTCCCTGTTT GCCAAACCCC TGCTTGCCGA ACAAACCCTG ACCGTCAACG 551 GCAACACCGT CTCCGCCAAC GGCGGCGGCT GGCAGGTACT GGATACGGGC 601 GCGGCACTGC CCCTGACCAT ACACACCGAA ATGCCGTGGG ACATCGGCTT 651 CATCAACATC GAAAATCCCG CCGGCGGCAT TACCGTTTCC GCGATGGGCA 701 TCAACGGCGC ACAATTAACC CAGTGGTCGA AATGGCGTGC CGACCGTATG 751 AACGACCTCG CCCAAACCGG CGCCGATTTG GTTATCCTTT CCTACGGCAC 801 CAACGAAGCT TTCAACAACA ACATCGACAT TGCCGACACC GAACAAAAAT 851 GGCTGGATAC CGTCCGCCAA ATCCGCGACA GCCTGCCTGC CGCCGGCATC 901 CTCATCATCG GCGCACCCGA ATCCCTGAAA AACACGCTCG GCGTATGCGG 951 CACACGCCCC GTCCGCCTGA CCGAAGTCCA ACAGATGCAG CGGCGCGTCG 1001 CCCGTCAGGG GCAGACGATG TTCTGGTCTT GGCAAAACGC CATGGGCGGC 1051 ATATGCAGCA TGAAAAACTG GCTCAACCAA GGATGGGCCG CCAAAGACGG 1101 CGTACACTTC TCCGCCAAAG GCTACCGGCG CGCGGCGGAA ATGCTCGCCG 1151 ACAGCCTCGA AGAACTCGTC CGCTCCGCTG CAATCAGGCA ATAA This corresponds to the amino acid sequence <SEQ ID 1250; ORF 299>: m299.pep 1 MΝPKHLIAFS ALFAATQAEA LPVASVSLDT VTVSPSAPYT DTNGLLTDYG 51 NASASPWMKK LQSVAQGSGE TFRILQIGDS HTAGDFFTDS LRKRLQKTWG 101 DGGIGWVYPA NVKGQRMAAV RHNGNWQSLT SRNNTGDFPL GGILAHTGSG 151 GSMTLTASDG IASKQRVSLF AKPLLAEQTL TVNGNTVSAN GGGWQVLDTG 201 AALPLTIHTE MPWDIGFINI ENPAGGITVS AMGINGAQLT QWSKWRADRM 251 NDLAQTGADL VILSYGTNEA FNNNIDIADT EQKWLDTVRQ IRDSLPAAGI 301 LIIGAPESLK NTLGVCGTRP VRLTEVQQMQ RRVARQGQTM FWSWQNAMGG 351 ICSMKNWLNQ GWAAKDGVHF SAKGYRRAAE MLADSLEELV RSAAIRQ* m299/g299 95.5% identity in 397 aa overlap 10 20 30 40 50 60 m299.pep MNPKHLIAFSALFAATQAEALPVASVSLDTVTVSPSAPYTDTNGLLTDYGNASASPWMKK g299 MNPKHFIAFSALFAATQAEALPVASVSPDTVTVSPSAPYTDTNGLLTDYGNAAASPWMKK 10 20 30 40 50 60 70 80 90 100 110 120 m299.pep LQSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDGGIGWVYPANVKGQRMAAV g299 LRSVAQGSGEAFRILQIGDSHTAGDFFTDALRKRLQKTWGDGGIGWVYPANVKGQRMAAV 70 80 90 100 110 120 130 140 150 160 170 180 m299.pep RHNGNWQSLTSRNNTGDFPLGGILAHTGSGGSMTLTASDGIASKQRVΞLFAKPLLAEQTL I h l l l l M I I I I I M I I I M I I I h l l l l h l l M I I I I g299 RHSGNWQSFTSRNNTGDFPLGGILAQTGSGGGMTLTASDGKTGKQRVΞLFAKPLLAEQTL 130 140 150 160 170 180 190 200 210 220 230 240 m299.pep TVNGNTVSANGGGWQVLDTGAALPLTIHTEMPWDIGFINIENPAGGITVSAMGINGAQLT g299 TVNGNTVΞANGGGWQVLDTGAALPLAIQTEMPWDIGFINIENPAGGITVSAMGINGAQLT 190 200 210 220 230 240 250 260 270 280 290 300 m299.pep QWSKWRADRMNDLAQTGADLVILSYGTNEAFNNNIDIADTEQKWLDTVRQIRDSLPAAGI g299 QWSKWRADRMNDLAQTGADLVILSYGTNEAFNNNIDIADTEQKWLDTVRQIRDSLPAAGI 250 260 270 280 290 300 310 320 330 340 350 360 m299.pep LIIGAPESLKNTLGVCGTRPVRLTEVQQMQRRVARQGQTMFWSWQNAMGGICSMKNWLNQ g299 LIIGAPEΞLKNTLGVCGTRPVLLTEVQQMQRRVARQGQTMFWSWQNAMGGICSMKNWLNQ 310 320 330 340 350 360 370 380 390 m299. pep GWAAKDGVHFSAKGYRRAAEMLADSLEELVRSAAIRQX g299 GWAAKDGVHFSAQGYRRAAEMLADSLEELVRAAAIRQX 370 380 390 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1251>: a299.seq 1 ATGAACCCCA AACACCTCAT CGCATTTTCC GCCCTATTCG CCGCCACGCA 51 GGCAGAAGCC CTACCTGTCG CCTCAGTCAG CCTCGACACC GTTACCGTTT 101 CCCCGTCCGC CCCCTACACC GATACAAACG GGCTGCTGAC CGACTACGGC 151 AACGCCTCCG CCTCGCCTTG GATGAAAAAA CTCCAATCCG TCGCACAAGG 201 CAGCGGCGAG ACCTTCCGTA TCCTGCAAAT CGGCGACTCG CATACCGCCG 251 GCGACTTCTT TACCGACAGC CTGCGCAAAC GCCTACAAAA AACTTGGGGC 301 GACGGCGGCA TAGGCTGGGT TTACCCCGCC AACGTCAAAG GGCAGCGCAT 351 GGCGGCCGTC CGGCACAACG GTAACTGGCA AAGCCTCACC AGCAGGAACA 401 ACACCGGAGA CTTCCCGCTC GGCGGCATCC TCGCCCACAC CGGCAGCGGC 451 GGCAGCATGA CCCTGACCGC ATCGGACGGC ATAGCAAGCA AGCAGCGCGT 501 TTCCCTGTTT GCCAAACCCC TGCTTGCCGA ACAAACCCTG ACCGTCAACG 551 GCAACACCGT CTCCGCCAAC GGCGGCGGCT GGCAGGTACT GGATACGGGC 601 GCGGCACTGC CCCTGACCAT ACACACCGAA ATGCCGTGGG ACATCGGCTT 651 CATCAACATC GAAAATCCCG CCGGCGGCAT TACCGTTTCC GCGATGGGCA 701 TCAACGGCGC ACAATTAACC CAGTGGTCGA AATGGCGTGC CGACCGTATG 751 AACGACCTTG CCCAAACCGG CGCCGATCTA GTCATCCTTG CCTACGGTAC 801 CAACGAAGCC TTCGGCGACA ACATCGACAT TGCCGATACC GAACAGAAAT 851 GGCTGGATAC CGTCCGCCAA ATCCGCGACA GCCTACCTGC CGCCGGCATC 901 CTCATCATCG GCGCGCCCGA ATCCCTGAAA AACACGCTCG GCGTATGCGG 951 CACACGCCCC GTCCGCCTGA CCGAAGTCCA ACAGATGCAG CGGCGCATCG 1001 CCCGTCAGGG GCAGACGATG TTCTGGTCTT GGCAAAACGC GATGGGCGGC 1051 GTTTGCAGCA TGAAAAACTG GCTCAACCAC GGATGGGCCG CCAAAGACGG 1101 CGTACACTTT TCCGCCAAAG GCTACCAACG GTCGGCGGAA ATGCTCGCCG 1151 ACAGCCTCGA AGAACTCGTC CGCTCCGCTG CAATCAGGCA ATAA This corresponds to the amino acid sequence <SEQ ID 1252; ORF 299. a>: a299.pep 1 MNPKHLIAFS ALFAATQAEA LPVASVSLDT VTVSPSAPYT DTNGLLTDYG 51 NASASPWMKK LQSVAQGSGE TFRILQIGDS HTAGDFFTDS LRKRLQKTWG 101 DGGIGWVYPA NVKGQRMAAV RHNGNWQSLT SRNNTGDFPL GGILAHTGSG 151 GSMTLTASDG IASKQRVSLF AKPLLAEQTL TVNGNTVSAN GGGWQVLDTG 201 AALPLTIHTE MPWDIGFINI ENPAGGITVS AMGINGAQLT QWSKWRADRM 251 NDLAQTGADL VILAYGTNEA FGDNIDIADT EQKWLDTVRQ IRDSLPAAGI 301 LIIGAPESLK NTLGVCGTRP VRLTEVQQMQ RRIARQGQTM FWSWQNAMGG 351 VCSMKNWLNH GWAAKDGVHF SAKGYQRSAE MLADSLEELV RSAAIRQ* m299/a299 98.0% identity in 397 aa overlap 10 20 30 40 50 60 m299. pep MNPKHLIAFSALFAATQAEALPVASVSLDTVTVSPSAPYTDTNGLLTDYGNASASPWMKK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a299 MNPKHLIAFSALFAATQAEALPVASVSLDTVTVSPSAPYTDTNGLLTDYGNASASPWMKK 10 20 30 40 50 60 70 80 90 100 110 120 m299.pep LQSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDGGIGWVYPANVKGQRMAAV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a299 LQSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDGGIGWVYPANVKGQRMAAV 70 80 90 100 110 120 130 140 150 160 170 180 m299.pep RHNGNWQSLTSRNNTGDFPLGGILAHTGSGGSMTLTASDGIASKQRVSLFAKPLLAEQTL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a299 RHNGNWQSLTSRNNTGDFPLGGILAHTGSGGSMTLTASDGIASKQRVSLFAKPLLAEQTL 130 140 150 160 170 180 190 200 210 220 230 240 m299.pep TVNGNTVSANGGGWQVLDTGAALPLTIHTEMPWDIGFINIENPAGGITVSAMGINGAQLT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a299 TVNGNTVSANGGGWQVLDTGAALPLTIHTEMPWDIGFINIENPAGGITVSAMGINGAQLT 190 200 210 220 230 240 250 260 270 280 290 300 m299.pep QWSKWRADRMNDLAQTGADLVILSYGTNEAFNNNIDIADTEQKWLDTVRQIRDSLPAAGI I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I :: I I I I I I I I I I I I I I I I I I I I I I I I I I I a299 QWSKWRADRMNDLAQTGADLVILAYGTNEAFGDNIDIADTEQKWLDTVRQIRDSLPAAGI 250 260 270 280 290 300 310 320 330 340 350 360 m299.pep LIIGAPESLKNTLGVCGTRPVRLTEVQQMQRRVARQGQTMFWSWQNAMGGICSMKNWLNQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I : I I I I I I I I : a299 LIIGAPESLKNTLGVCGTRPVRLTEVQQMQRRIARQGQTMFWSWQNAMGGVCSMKNWLNH 310 320 330 340 350 360 370 380 390 m299. pep GWAAKDGVHFSAKGYRRAAEMLADSLEELVRSAAIRQX I I I I I I I I I I I I I I I : I : I II I I I I I I I I I I I I I I I I I a299 GWAAKDGVHFSAKGYQRSAEMLADSLEELVRSAAIRQX 370 380 390 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1253>: g302. seq 1 ATGCACTCAA TATATTTTTT TAAGGAGAAG CAGATGAGTC AAACCGACGC 51 GCGTCGTAGC GGACGATTTT TACGCACAGT CGAATGGCTG GGCAATATGT 101 TGCCGCACCC GGTTACGCTT TTTATTATTT TCATTGTGTT ATTGCTGATT 151 GCCTCTGCCG TCGGTGCGTA TTTCGGACTA TCCGTCCCCG ATCCGCGTCC 201 TGTTGGGGCG AAAGGACGTG CCGATGACGG TTTGATTCAC GTTGTCAGCC 251 TGCTCGATGC CGACGGTTTG ATCAAAATCC TGACGCATAC CGTTAAAAAT 301 TTCACCGGTT TCGCGCCGTT GGGAACGGTG TTGGTTTCTT TATTGGGCGT 351 GGGGATTGCG GAAAAATCGG GCTTGATTTC CGCATTAATG CGCTTATTGC 401 TCACAAAATC CCCACGCAAA CTCACTACTT TTATGGTTGT TTTTACAGGG 451 ATTTTATCCA ATACGGCTTC TGAATTGGGC TATGTCGTCC TAATCCCTTT 501 GTCCGCCGTC ATCTTTCATT CGCTCGGCCG CCATCCGCTT GCCGGTTTGG 551 CTGCGGCTTT CGCCGGCGTT TCGGGCGGTT ATTCGGCCAA TCTGTTCTTA 601 GGCACAATCG ATCCGCTCTT GGCAGGCATC ACCCAACAGG CGGCGCAAAT 651 CATCCATCCC GACTACGTCG TAGGCCCTGA AGCCAACTGG TTTTTTATGG 701 CAGCCAGTAC GTTTGTGATT GCTTTGATTG GTTATTTTGT TACTGAAAAA 751 ATCGTCGAAC CGCAATTGGG CCCTTATCAA TCAGATTTGT CACAAGAAGA 801 AAAAGACATT CGGCATTCCA ATGAAATCAC GCCTTTGGAA TATAAAGGAT 851 TAATTTGGGC AGGCGTGGTG TTTGTTGCCT TATCCGCCCT ATTGGCTTGG 901 AGCATCGTCC CTGCCGACGG TATTTTGCGT CATCCTGAAA CAGGATTGGT 951 TGCCGGTTCG CCGTTTTTAA AATCGATTGT TGTTTTTATT TTCTTGTTGT 1001 TTGCGCTGCC GGGCATTGTT TATGGCCGGA TAACCCGAAG TTTGCGCGGC 1051 GAACGGGAAG TCGTTAATGC GATGGCCGAA TCGATGAGTA CTTTGGGACT 1101 TTATTTGGTC ATCATCTTTT TTGCCGCACA GTTTGTCGCA TTTTTTAATT 1151 GGACGAATAT TGGGCAATAT ATTGCCGTTA AAGGGGCGGT GTTCTTAAAA 1201 GAAGTCGGCT TGGGCGGCAG TGTGTTGTTT ATCGGTTTTA TTTTAATTTG 1251 TGCTTTTATC AATCTGATGA TAGGCTCCGC CTCCGCGCAA TGGGCGGTAA 1301 CTGCGCCGAT TTTCGTCCCT ATGCTGATGT TGGCCGGCTA CGCGCCCCAA 1351 GTCATTCAAG CCGCTTACCG CATCGGTGAT TCCGTTACCA ATATTATTAC 1401 GCCGATGATG AGTTATTTCG GGCTGATTAT GGCGACGGTA ATCAAATACA 1451 AAAAAGATGC GGGCGTAGGC ACGCTGATTT CTATGATGTT GCCGTATTCC 1501 GCTTTCTTCT TAATTGCATG GATCGCCTTA TTCTGCATTT GGGTATTTGT 1551 TTTGGGTCTG CCCGTCGGTC CCGGCACACC CACATTCTAT CCGGTGCCTT 1601 AA This corresponds to the amino acid sequence <SEQ ID 1254; ORF 302.ng>: g30 -pep 1 MHSIYFFKEK QMSQTDARRS GRFLRTVEWL GNMLPHPVTL FIIFIVLLLI 51 ASAVGAYFGL SVPDPRPVGA KGRADDGLIH WSLLDADGL IKILTHTVKN 101 FTGFAPLGTV LVSLLGVGIA EKSGLISALM RLLLTKSPRK LTTFMWFTG 151 ILSNTASELG YWLIPLSAV IFHSLGRHPL AGLAAAFAGV SGGYSANLFL 201 GTIDPLLAGI TQQAAQIIHP DYWGPEANW FFMAASTFVI ALIGYFVTEK 251 IVEPQLGPYQ SDLSQEEKDI RHSNEITPLE YKGLIWAGW FVALSALLAW 301 SIVPADGILR HPETGLVAGS PFLKSIWFI FLLFALPGIV YGRITRSLRG 351 EREWNAMAE SMSTLGLYLV IIFFAAQFVA FFNWTNIGQY IAVKGAVFLK 401 EVGLGGSVLF IGFILICAFI NLMIGSASAQ WAVTAPIFVP MLMLAGYAPQ 451 VIQAAYRIGD SVTNIITPMM SYFGLIMATV IKYKKDAGVG TLISMMLPYS 501 AFFLIAWIAL FCIWVFVLGL PVGPGTPTFY PVP* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1255>: m302. seq 1 ATGCACTCAA TATATTTTTT TAAGGAGAAG CAGATGAGTC AAACCGATAC 51 GCAACGGGAC GGACGATTTT TACGCACAGT CGAATGGCTG GGCAATATGT 101 TGCCGCATCC GGTTACGCTT TTTATTATTT TCATTGTGTT ATTGCTGATT 151 GCCTCTGCCG TCGGTGCGTA TTTCGGACTA TCCGTCCCCG ATCCGCGCCC 201 TGTTGGTGCG AAAGGACGTG CCGATGACGG TTTGATTTAC ATTGTCAGCC 251 TGCTCAATGC CGACGGTTTT ATCAAAATCC TGACGCATAC CGTTAAAAAT 301 TTCACCGGTT TCGCGCCGTT GGGAACGGTG TTGGTTTCTT TATTGGGCGT 351 GGGGATTGCG GAAAAATCGG GCTTGATTTC CGCATTAATG CGCTTATTGC 401 TCACAAAATC GCCACGCAAA CTCACTACTT TTATGGTTGT TTTTACAGGG 451 ATTTTATCTA ATACCGCTTC TGAATTGGGC TATGTCGTCC TAATCCCTTT 501 GTCCGCCATC ATCTTTCATT CCCTCGGCCG CCATCCGCTT GCCGGTCTGG 551 CTGCGGCTTT CGCCGGCGTT TCGGGCGGTT ATTCGGCCAA TCTGTTCTTA 601 AGCACAATCG ATCCGCTCTT GGCATGCATC ACCCATCAGG CGGCGGTCGT 651 AGGCCCTGAA GCCAACTGGT TTTTTATGGT AGCCAGTACG TTTGTGATTG 701 CTTTGATTGG TTATTTTGTT ACTGAAAAAA TCGTCGAACC GCAATTGGGC 751 CCTTATCAAT CAGATTTGTC ACAAGAAGAA AAAGACATTC GGCATTCCAA 801 TGAAATCACG CCTTTGGAAT ATAAAGGATT AATTTGGGCT GGCGTGGTGT 851 TTGTTGCCTT ATCCGCCCTA TTGGCTTGGA GCATCGTCCC TGCCGACGGT 901 ATTTTGCGTC ATCCTGAAAC AGGATTGGTT TCCGGTTCGC CGTTTTTAAA 951 ATCGATTGTT GTTTTTATTT TCTTGTTGTT TGCACTGyCG GGCmTTGTTT 1001 ATGGmCGGGT AACCCGAAGT TTGCGCGGCG AACAGGAAGT CGTTAATGCG 1051 ATGGCCGAAT CGATGAGTAC TCTGGsGCTT TmTTTGswCA kcATCTTTTT 1101 TGCCGCACAG TTTGTCGCAT TTTTTAATTG GACGAATATT GGGCAATATA 1151 TTGCCGTTAA AGGGGCGACG TTCTTAAAAG AAGTCGGCTT GGGCGGCAGC 1201 GTGTTGTTTA TCGGTTTTAT TTTAATTTGT GCTTTTATCA ATCTGATGAT 1251 AGGCTCCGCC TCCGCGCAAT GGGCGGTAAC TGCGCCGATT TTCGTCCCTA 1301 TGCTGATGTT GGCCGGCTAC GCGCCCGAAG TCATTCAAGC CGCTTACCGC 1351 ATCGGTGATT CCGTTACCAA TATTATTACG CCGATGATGA GTTATTTCGG 1401 GCTGATTATG GCGACGGTGA TCAAATACAA AAAAGATGCG GGCGTGGGTA 1451 CGCTGATTTC TATGATGTTG CCGTATTCCG CTTTCTTCTT GATTGCGTGG 1501 ATTGCCTTAT TCTGCATTTG GGTATTTGTT TTGGGCCTGC CCGTCGGTCC 1551 CGGCGCGCCC ACATTCTATC CCGCACCTTA A This corresponds to the amino acid sequence <SEQ ID 1256; ORF 302>: m302.pep 1 MHSIYFFKEK QMSQTDTQRD GRFLRTVEWL GNMLPHPVTL FIIFIVLLLI 51 ASAVGAYFGL SVPDPRPVGA KGRADDGLIY IVSLLNADGF IKILTHTVKN 101 FTGFAPLGTV LVSLLGVGIA EKSGLISALM RLLLTKSPRK LTTFMWFTG 151 ILSNTASELG YWLIPLSAI IFHSLGRHPL AGLAAAFAGV SGGYSANLFL 201 STIDPLLACI THQAAWGPE ANWFFMVAST FVIALIGYFV TEKIVEPQLG 251 PYQSDLSQEE KDIRHSNEIT PLEYKGLIWA GWFVALSAL LAWSIVPADG 301 ILRHPETGLV SGSPFLKSIV VFIFLLFALX GXVYGRVTRS LRGEQEWNA 351 MAESMSTLXL XLXXIFFAAQ FVAFFNWTNI GQYIAVKGAT FLKEVGLGGS 401 VLFIGFILIC AFINLMIGSA SAQWAVTAPI FVPMLMLAGY APEVIQAAYR 451 IGDSVTNIIT PMMSYFGLIM ATVIKYKKDA GVGTLISMML PYSAFFLIAW 501 IALFCIWVFV LGLPVGPGAP TFYPAP* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 302 shows 94.0% identity over a 533 aa overlap with a predicted ORF (ORF 302.ng) from N gonorrhoeae: m302/g302 10 20 30 40 50 60 m302.pep MHSIYFFKEKQMSQTDTQRDGRFLRTVEWLGΝMLPHPVTLFIIFIVLLLIASAVGAYFGL I M MI I I i ; :: M ! M M Ml MI M I I I I M MM M MM g302 MHSIYFFKEKQMSQTDARRSGRFLRTVEWLGΝMLPHPVTLFIIFIVLLLIASAVGAYFGL 10 20 30 40 50 60 70 80 90 100 110 120 m302. pep SVPDPRPVGAKGRADDGLIYIVSLLΝADGFIKILTHTVKΝFTGFAPLGTVLVSLLGVGIA I MM : M M : !| : II I I I i I , I M I g302 SVPDPRPVGAKGRADDGLIHWSLLDADGLIKILTHTVKΝFTGFAPLGTVLVSLLGVGIA 70 80 90 100 110 120 130 140 150 160 170 180 m302.pep EKSGLISALMRLLLTKSPRKLTTFMWFTGILSΝTASELGYWLIPLSAIIFHSLGRHPL 11111 i J 1 E 1111 E 11111111 i E 111 i 11111 E ! M 11111111111 ! = 11 F 111111 E g302 EKSGLISALMRLLLTKSPRKLTTFMWFTGILSΝTASELGYWLIPLSAVIFHSLGRHPL 130 140 150 160 170 180 190 200 210 220 230 m302. pep AGLAAAFAGVSGGYSAΝLFLSTIDPLLACITHQAA WGPEAΝWFFMVASTFVI 11111 i 1111 II I IE !!! 11 ^ E 111111 Ihlll 11 II 11111 II = 1111 II g302 AGLAAAFAGVSGGYSAΝLFLGTIDPLLAGITQQAAQIIHPDYWGPEAΝWFFMAASTFVI 190 200 210 220 230 240 240 250 260 270 280 290 m302. pep ALIGYFVTEKIVEP Q LGPY Q SDLSQEEKDIRHSΝEITPLEYKGLIWAGWFVALSALLAW I I I MM II M IMI I III III II II M I III M II III I I II M II I I I I I M I M I I I g302 ALIGYFVTEKIVEP Q LGPY Q SDLSQEEKDIRHΞΝEITPLEYKGLIWAGWFVALSALLAW 250 260 270 280 290 300 300 310 320 330 340 350 m302 . pep SIVPADGILRHPETGLVSGSPFLKSIWFIFLLFALXGXVYGRVTRSLRGEQEWNAMAE I M l h l l M M M M M M M g302 SIVPADGILRHPETGLVAGSPFLKSIWFIFLLFALPGIVYGRITRSLRGEREWNAMAE 310 320 330 340 350 360 360 370 380 390 400 410 m302 . pep SMSTLXLXLXXIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVGLGGSVLFIGFILICAFI I M M I I 1 1 1 E 1 1 1 1 1 f 1 1 1 E 1 1 1 1 i E 1 1 1 1 i = 1 1 E 1 1 1 1 1 1 1 J 1 1 E 1 1 1 E I E I E I g302 SMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGAVFLKEVGLGGSVLFIGFILICAFI 370 380 390 400 410 420 420 430 440 450 460 470 m302.pep NLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDSVTNIITPMMSYFGLIMATV E 111 ! I i 111111 ! 111 i 11 i 11111 i 11 = M 111111 ! I ! I ! 11111111 ! M 111 ! 11 g3 o2 NLMIGSASAQWAVTAPIFVPMLMLAGYAPQVIQAAYRIGDSVTNI ITPMMSYFGLIMATV 430 440 450 460 470 480 480 490 500 510 520 m302. pep IKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLGLPVGPGAPTFYPAPX M M M M M M M M M M M M M M M M M I M M M M I h M l l h l l g302 IKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLGLPVGPGTPTFYPVPX 490 500 510 520 530 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1257>: a302. seq 1 ATGCACTCAA TATATTTTTT TAAGGAGAAG CAGATGAGTC AAACCGATAC 51 GCAACGGGAC GGACGATTTT TACGCACAGT CGAATGGCTG GGCAATATGT 101 TGCCGCACCC GGTTACGCTT TTTATTATTT TCATTGTGTT ATTGCTGATT 151 GCCTCTGCCG CCGGTGCGTA TTTCGGACTA TCCGTCCCCG ATCCGCGCCC 201 TGTTGGTGCG AAAGGACGTG CCGATGACGG TTTGATTCAC GTTGTCAGCC 251 TGCTCGATGC TGACGGTTTG ATCAAAATCC TGACGCATAC CGTTAAAAAT 301 TTCACCGGTT TCGCGCCGTT GGGAACGGTG TTGGTTTCTT TATTGGGCGT 351 GGGGATTGCG GAAAAATCGG GCTTGATTTC CGCATTAATG CGCTTATTGC 401 TCACAAAATC TCCACGCAAA CTCACTACTT TTATGGTTGT TTTTACAGGG 451 ATTTTATCTA ATACCGCTTC TGAATTGGGC TATGTCGTCC TAATCCCTTT 501 GTCCGCCATC ATCTTTCATT CCCTCGGCCG CCATCCGCTT GCCGGTCTGG 551 CTGCGGCTTT CGCCGGCGTT TCGGGCGGTT ATTCGGCCAA TCTGTTCTTA 601 GGCACAATCG ATCCGCTCTT GGCAGGCATC ACCCAACAGG CGGCGCAAAT 651 CATCCATCCC GACTACGTCG TAGGCCCTGA AGCCAACTGG TTTTTTATGG 701 TAGCCAGTAC GTTTGTGATT GCTTTGATTG GTTATTTTGT TACTGAAAAA 751 ATCGTCGAAC CGCAATTGGG CCCTTATCAA TCAGATTTGT CACAAGAAGA 801 AAAAGACATT CGACATTCCA ATGAAATCAC GCCTTTGGAA TATAAAGGAT 851 TAATTTGGGC TGGCGTGGTG TTTGTTGCCT TATCCGCCCT ATTGGCTTGG 901 AGCATCGTCC CTGCCGACGG TATTTTGCGT CATCCTGAAA CAGGATTGGT 951 TTCCGGTTCG CCGTTTTTAA AATCAATTGT TGTTTTTATT TTCTTGTTGT 1001 TTGCACTGCC GGGCATTGTT TATGGCCGGG TAACCCGAAG TTTGCGCGGC 1051 GAACAGGAAG TCGTTAATGC GATGGCCGAA TCGATGAGTA CTCTGGGGCT 1101 TTATTTGGTC ATCATCTTTT TTGCCGCACA GTTTGTCGCA TTTTTTAATT 1151 GGACGAATAT TGGGCAATAT ATTGCCGTTA AAGGGGCGAC GTTCTTAAAA 1201 GAAGTCGGCT TGGGCGGCAG CGTGTTGTTT ATCGGTTTTA TTTTAATTTG 1251 TGCTTTTATC AATCTGATGA TAGGCTCCGC CTCCGCGCAA TGGGCGGTAA 1301 CTGCGCCGAT TTTCGTCCCT ATGCTGATGT TGGCCGGCTA CGCGCCCGAA 1351 GTCATTCAAG CCGCTTACCG CATCGGTGAT TCCGTTACCA ATATTATTAC 1401 GCCGATGATG AGTTATTTCG GGCTGATTAT GGCGACGGTG ATCAAATACA 1451 AAAAAGATGC GGGCGTGGGT ACGCTGATTT CTATGATGTT GCCGTATTCC 1501 GCTTTCTTCT TGATTGCGTG GATTGCCTTA TTCTGCATTT GGGTATTTGT 1551 TTTGGGCCTG CCCGTCGGTC CCGGCGCGCC CACATTCTAT CCCGCACCTT 1601 AA This corresponds to the amino acid sequence <SEQ ID 1258; ORF 302. a>: a302.pep 1 MHSIYFFKEK QMSQTDTQRD GRFLRTVEWL GNMLPHPVTL FIIFIVLLLI 51 ASAAGAYFGL SVPDPRPVGA KGRADDGLIH VVSLLDADGL IKILTHTVKN 101 FTGFAPLGTV LVSLLGVGIA EKSGLISALM RLLLTKSPRK LTTFMWFTG 151 ILSNTASELG YVVLIPLSAI IFHSLGRHPL AGLAAAFAGV SGGYSANLFL 201 GTIDPLLAGI TQQAAQIIHP DYWGPEANW FFMVASTFVI ALIGYFVTEK 251 IVEPQLGPYQ SDLSQEEKDI RHSNEITPLE YKGLIWAGW FVALSALLAW 301 SIVPADGILR HPETGLVSGS PFLKSIWFI FLLFALPGIV YGRVTRSLRG 351 EQEWNAMAE SMSTLGLYLV IIFFAAQFVA FFNWTNIGQY IAVKGATFLK 401 EVGLGGSVLF IGFILICAFI NLMIGSASAQ WAVTAPIFVP MLMLAGYAPE 451 VIQAAYRIGD SVTNIITPMM SYFGLIMATV IKYKKDAGVG TLISMMLPYS 501 AFFLIAWIAL FCIWVFVLGL PVGPGAPTFY PAP* m302/a302 96.1 % identity in 533 aa overlap 10 20 30 40 50 60 m302. pep MHSIYFFKEKQMSQTDTQRDGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAVGAYFGL I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I a302 MHSIYFFKEKQMSQTDTQRDGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAAGAYFGL 10 20 30 40 50 60 70 80 90 100 110 120 m302.pep SVPDPRPVGAKGRADDGLIYIVSLLNADGFIKILTHTVKNFTGFAPLGTVLVSLLGVGIA I I I I I I I I I I I I I I I I I I I :: I I I I : I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a302 SVPDPRPVGAKGRADDGLIHWSLLDADGLIKILTHTVKNFTGFAPLGTVLVSLLGVGIA 70 80 90 100 110 120 130 140 150 160 170 180 m302. pep EKSGLISALMRLLLTKSPRKLTTFMWFTGILSNTASELGYWLIPLSAIIFHSLGRHPL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a302 EKSGLISALMRLLLTKSPRKLTTFMWFTGILSNTASELGYWLIPLSAIIFHSLGRHPL 130 140 150 160 170 180 190 200 210 220 230 m302. pep AGLAAAFAGVSGGYSANLFLSTIDPLLACITHQAA WGPEANWFFMVASTFVI I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I: I I I I I I I I I I I I I I I I I I I I I a302 AGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYWGPEANWFFMVASTFVI 190 200 210 220 230 240 240 250 260 270 280 290 m302.pep ALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWAGWFVALSALLAW I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a302 ALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWAGVVFVALSALLAW 250 260 270 280 290 300 300 310 320 330 340 350 m302. pep SIVPADGILRHPETGLVSGSPFLKSIWFIFLLFALXGXVYGRVTRSLRGEQEWNAMAE I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a302 SIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVTRSLRGEQEWNAMAE 310 320 330 340 350 360 360 370 380 390 400 410 m302.pep SMSTLXLXLXXIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVGLGGSVLFIGFILICAFI I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a302 SMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVGLGGSVLFIGFILICAFI 370 380 390 400 410 420 420 430 440 450 460 470 m302. pep NLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDSVTNIITPMMSYFGLIMATV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a302 NLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDSVTNIITPMMSYFGLIMATV 430 440 450 460 470 480 480 490 500 510 520 m302.pep IKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLGLPVGPGAPTFYPAPX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M I a302 IKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLGLPVGPGAPTFYPAPX 490 500 510 520 530 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1259>: g305 . seq 1 ATGGATTTTT TGATTGTCCT GAAAGCCCTG ATGATGGGCT TGGTAGAAGG 51 TTTTACCGAA TTTTTACCGA TTTCCAGCAC CGGACATTTG ATTGTGTTCG 101 GCAATCTGAT TGGTTTTCAC AGCAATCACA AGGTTTTTGA AATTGCCATC 151 CAGCTCGGTG CGGTTTTGGC GGTAGTGTTT GAATACCGGC AGCGTTTCAG 201 CAATGTGTTG CATGGCGTGG GAAAAGACCG GAAAGCCAAC CGTTTCGTCC 251 TCAATCTTGC CATTGCTTTT ATACCTGCCG CCGTGATGGG GCTGTTGTTC 301 GACAAACAAA TCAAAGAGTA TCTGTTTAAC CCCTTGAGTG TTGCAGTCAT 351 GCTGGTTTTG GGCGGTTTTT TTATTTTGTG GGTGGAGAAA CGCCAAAGCC 401 GAGCAGAGCC TAAAATTGCC GATGTTGATG CATTGCGTCC GATTGATGCG 451 TTGATGATCG GTGTTGCCCA AGTGTTTGCA CTGGTTCCGG GTACGTCCCG 501 TTCGGGCAGT ACGGTTATGG GCGGGATGCT TTGGGGAATC GAGCGGAAAA 551 CGGCAACGGA GTTTTCATTT TTCTTGGCCG TTCCGATGAT GGTTGCAGCA 601 ACGGCTTATG ATGTCCTGAA ACATTACCGA TTTTTCACCC TGCATGATGT 651 CGGTTTGATT TTGATAGGCT TTATTGCCGC TTTTGTTTCC GGTTTGGTAG 701 CGGTTAAAGC ACTGCTGAAG TTTGTTTCCA AGAAAAACTA TATCCCGTTT 751 GCCTATTACC GCATTGTTTT CGGCATTGTC ATCATAATAT TGTGGTTGTC 801 GGGCTGGATA AGTTGGGAAT GA This corresponds to the amino acid sequence <SEQ ID 1260; ORF 305.ng>: g305.pep 1 MDFLIVLKAL MMGLVEGFTE FLPISSTGHL IVFGNLIGFH SNHKVFEIAI 51 QLGAVLAWF EYRQRFSNVL HGVGKDRKAN RFVLNLAIAF IPAAVMGLLF 101 DKQIKEYLFN PLSVAVMLVL GGFFILWVEK RQSRAEPKIA DVDALRPIDA 151 LMIGVAQVFA LVPGTSRSGS TVMGGMLWGI ERKTATEFSF FLAVPMMVAA 201 TAYDVLKHYR FFTLHDVGLI LIGFIAAFVS GLVAVKALLK FVSKKNYIPF 251 AYYRIVFGIV IIILWLSGWI SWE* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 1261>: m305.seq (partial) 1 AtGGATTTTC TGATTGTCCT GAAAGCCCTG ATGATGGGCT TGGTAGAAGG 51 TTTTACCGAA TTTTTACCGA TTTCCAGCAC CGGACATTTG ATTGTGTTCG 101 GCAATCTGAT TGGTTTTCAC AGCAATCACA AGGTTTTTGA AATTGCCATC 151 CAGCTCGGTG CAGTTTTGGC GGTAGTGTTT GAATACCGGC AACGTTTCAG 201 CAATGTGTTG CACGGCTTGG GAAAAGACCG GAAAGCCAAC CGCTTCGTCC 251 TTAATCTTGC CATTGCTTTT ATACCTGCCG CCGTGATGGG GCTGTTGTTC 301 GGCAwACAAA TCAAAGAGyA TCTGTTTAAC CCCTTGAGTG TTGCAGTCAT 351 GCTGGTTyTG GrCGGTTTTT yTATTTTGTG GGTGGAGAAA CGCCAAAGCC 401 GAGCAGAGCC TAAAATTGCC GATGTTGATG CATTGCGTCC GATTGATGCC 451 TTGATGATCG GCGTTGCCCA AGTGTTTGCA CTGGTTCCGG GTACGTCCCG 501 TTCGGGCAGT ACGATTATGG GCGGGATGCT TTGGGGCATC GAACGGAAAA 551 CTGCGACAGA ATTCTCGTTT TTCTTGGCTG TGCCGATGAT GGTTGCCGCA 601 ACGGCTTATG ATGTCCTGAA ACATTACCGA TTTTTCACCC TGCATGATGT 651 CGGTTTGATT CTGATAGGCT TTATTGCTGC CTTTGTTTCA GGCTTGGTAG 701 CGGTAAAAGC GTTGCTGAGG TTTGTTTCGG GTAC ... This corresponds to the amino acid sequence <SEQ ID 1262; ORF 305>: m305.pep (partial) 1 MDFLIVLKAL MMGLVEGFTE FLPISSTGHL IVFGNLIGFH SNHKVFEIAI 51 QLGAVLAWF EYRQRFSNVL HGLGKDRKAN RFVLNLAIAF IPAAVMGLLF 101 GXQIKEXLFN PLSVAVMLVL XGFXILWVEK RQSRAEPKIA DVDALRPIDA 151 LMIGVAQVFA LVPGTSRSGS TIMGGMLWGI ERKTATEFSF FLAVPMMVAA 201 TAYDVLKHYR FFTLHDVGLI LIGFIAAFVS GLVAVKALLR FVSG ... Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 305 shows 96.7% identity over a 243 aa overlap with a predicted ORF (ORF 305.ng) from N gonorrhoeae: g305/m305 10 20 30 40 50 60 g305.pep MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGΝLIGFHSΝHKVFEIAIQLGAVLAWF 11 II Ml I II I M I M II I Ii II M II 11 II Ml III III II II 1111 II 111 m305 MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGΝLIGFHSΝHKVFEIAIQLGAVLAWF 10 20 30 40 50 60 70 80 90 100 110 120 g305.pep EYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFDKQIKEYLFNPLSVAVMLVL l l l l M M I M M M M m305 EYRQRFSNVLHGLGKDRKANRFVLNLAIAFIPAAVMGLLFGXQIKEXLFNPLSVAVMLVL 70 80 90 100 110 120 130 140 150 160 170 180 g305 -pep GGFFILWVEKRQSRAEPKIADVDALRPIDALMIGVAQVFALVPGTSRSGSTVMGGMLWGI I I M M M M M M M M M M I M M M M M M M M M M M M I h l M M I M m305 XGFXILWVEKRQSRAEPKIADVDALRPIDALMIGVAQVFALVPGTSRSGSTIMGGMLWGI 130 140 150 160 170 180 190 200 210 220 230 240 g305. pep ERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVGLILIGFIAAFVSGLVAVKALLK MMMMMMMMMMMMMMMMMMMMMMMIMMMMMIh m305 ERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVGLILIGFIAAFVSGLVAVKALLR 190 200 210 220 230 240 250 260 270 g305.pep FVSKKNYIPFAYYRIVFGIVIIILWLSGWISWEX III m305 FVSG The following partial DNA sequence was identified in N meningitidis <SEQ ID 1263>: a305. seq 1 ATGGATTTTC TGATTGTCCT GAAAGCCCTG ATGATGGGCT TGGTAGAAGG 51 TTTTACCGAA TTTTTACCGA TTTCCAGCAC CGGACATTTG ATTGTGTTCG 101 GCAATCTGAT TGATTTTCAC AGCAATCACA AGGTTTTTGA AATTACCATC 151 CAGCTCGGTG CGGTTTTGGC GGTAGTGTTT GAATACCGGC AGCGTTTCAG 201 CAATGTGTTG CATGGCGTGG GAAAAGACCG GAAAGCCAAC CGTTTCGTCC 251 TTAATCTTGC CATTGCTTTT ATACCTGCCG CCGTGATGGG GCTGTTGTTC 301 GGCAAACAAA TCAAAGAGTA TCTGTTTAAC CCCTTGAGTG TTGCAGTCAT 351 GCTGGTTTTG GGCGGTTTTT TTATTTTGTG GGTGGAGAAA CGCCAAAGCC 401 GAGCAGAGCC TAAAATTGTC GATGTTGATG CATTGCGTCC GATTGATGCG 451 TTGATGATCG GCGTTGCCCA AGTGTTTGCA CTGGTTCCAG GTACGTCCCG 501 TTCGGGCAGT ACGATTATGG GCGGGATGCT TTGGGGAATC GAGCGGAAAA 551 CGGCAACGGA GTTTTCATTT TTCTTGGCCG TTCCGATGAT GGTTGCAGCA 601 ACGGCTTATG ATGTCCTGAA GCATTACCGG TTTTTCACCC TGCATGATGT 651 CGGTTTGATT TTGATTGGCT TTGTTGCTGC CTTTGTTTCA GGCTTGGTGG 701 CGGTCAAAGC GTTGCTGAGG TTTGTTTCCA AGAAAAATTA TATTCCTTTT 751 GCCTATTACC GCATTGTTTT TGGTATTGCC ATCATTATAT TGTGGCTGTC 801 AGGCTGGATA AGTTGGGAAT GA This corresponds to the amino acid sequence <SEQ ID 1264; ORF 305. a>: a305.pep 1 MDFLIVLKAL MMGLVEGFTE FLPISSTGHL IVFGΝLIDFH SΝHKVFEITI 51 QLGAVLAWF EYRQRFSNVL HGVGKDRKAN RFVLNLAIAF IPAAVMGLLF 101 GKQIKEYLFN PLSVAVMLVL GGFFILWVEK RQSRAEPKIV DVDALRPIDA 151 LMIGVAQVFA LVPGTSRSGS TIMGGMLWGI ERKTATEFSF FLAVPMMVAA 201 TAYDVLKHYR FFTLHDVGLI LIGFVAAFVS GLVAVKALLR FVSKKNYIPF 251 AYYRIVFGIA IIILWLSGWI SWE* m305/a305 96.3% identity in 243 aa overlap 10 20 30 40 50 60 m305.pep MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIGFHSNHKVFEIAIQLGAVLAWF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I: I I I I I I I I I I I a305 MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIDFHSNHKVFEITIQLGAVLAWF 10 20 30 40 50 60 70 80 90 100 110 120 m305.pep EYR Q RFSNVLHGLGKDRKANRFVLNLAIAFIPAAVMGLLFGXQIKEXLFNPLSVAVMLVL I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I llll I I I I I I I I I I I I I a305 EYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFGKQIKEYLFNPLSVAVMLVL 70 80 90 100 110 120 130 140 150 160 170 180 m305.pep XGFXILWVEKRQSRAEPKIADVDALRPIDALMIGVAQVFALVPGTSRSGSTIMGGMLWGI II I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a305 GGFFILWVEKRQSRAEPKIVDVDALRPIDALMIGVAQVFALVPGTSRSGSTIMGGMLWGI 130 140 150 160 170 180 190 200 210 220 230 240 m305. pep ERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVGLILIGFIAAFVSGLVAVKALLR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I a305 ERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVGLILIGFVAAFVSGLVAVKALLR 190 200 210 220 230 240 m305.pep FVSG I I I a305 FVSKKNYIPFAYYRIVFGIAIIILWLSGWISWEX 250 260 270 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1265>: g306. seq 1 ATGTTTATGA ACAAATTTTC CCAATCCGGA AAAGGTCTGT CCGGTTTCTT 51 CTTCGGTTTG ATACTGGCAA CGGTCATTAT TGCCGGTATT TTGCTTTATC 101 TGAACCAGGG CGGTCAAAAT GCGTTCAAAA TCCCGGCTCC GTCGAAGCAG 151 CCTGCAGAAA CGGAAATCCT GAAACTGAAA AACCAGCCTA AGGAAGACAT 201 CCAACCTGAA CCGGCCGATC AAAACGCCTT GTCCGAACCG GATGTTGCGA 251 AAGAGGCAGA GCAGTCGGAT GCGGAAAAAG CTGCCGACAA GCAGCCCGTT 301 GCCGACAAAG CCGACGAGGT TGAAGAAAAG GCGGGCGAGC CGGAACGGGA 351 AGAGCCGGAC GGACAGGCAG TGCGCAAGAA AGCACTGACT GAAGAGCGTG 401 AACAAACCGT CAGGGAAAAA GCGCAGAAGA AAGATGCCGA AACGGTTAAA 451 AAAAAAGCGG TAAAACCGTC TAAAGAAACA GAGAAAAAAG CTTCAAAAGA 501 AGAGAAAAAG GCGGCGAAAG AAAAAGTTGC ACCCAAACCG ACCCCGGAAC 551 AAATCCTCAA CAGCCGCAGT ATCGAAAAAG CGCGTAGTGC CGCTGCCAAA 601 GAAGTGCAGA AAATGAAAAA CTTTGGGCAA GGCGGAAGCC AACGCATTAT 651 CTGCAAATGG GCGCGTATGC CGAACCCCGG AGCGCGGAAG GGCAGCGTGC 701 CAAACTGGCA ATCTTGGGCA TATCTTCCGA AGTGGTCGGC TATCAGGCGG 751 GACATAAAAC GCTTTACCGC GTGCAAAGCG GCAATATGTC CGCCGATGCG 801 GTGA This corresponds to the amino acid sequence <SEQ ID 1266; ORF 306.ng>: g306 -pep 1 MFMΝKFSQSG KGLSGFFFGL ILATVIIAGI LLYLΝQGGQΝ AFKIPAPSKQ 51 PAETEILKLK ΝQPKEDIQPE PADQΝALSEP DVAKEAEQSD AEKAADKQPV 101 ADKADEVEEK AGEPEREEPD GQAVRKKALT EEREQTVREK AQKKDAETVK 151 KKAVKPSKET EKKASKEEKK AAKEKVAPKP TPEQILΝSRS IEKARSAAAK 201 EVQKMKΝFGQ GGSQRIICKW ARMPΝPGARK GSVPΝWQSWA YLPKWSAIRR 251 DIKRFTACKA AICPPMR* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1267>: m306.seq (partial) 1 ..GGTTTGTTCT TCGGTTTGAT ACTGGCGACG GTCATTATTG CCGGTATTTT 51 GTTTTATCTG AACCAGAGCG GTCAAAATGC GTTCAAAATC CCGGCTTCGT 101 CGAAGCAGCC TGCAGAAACG GAAATCCTGA AACCGmA AA CCAGCyTAAG 151 GAAGACATCC AACCTGA CC GGCCGATCAA AACGCCTTGT CCGAACCGGA 201 TGCTGCGACA GAGGCAGAGC AGTCGGATGC GGAAAAwGCT GCCGACAAGC 251 AGCCCGTTGC CGATAAAGCC GACGAGGTTG AAGAAAAGGC GGGCGAGCCG 301 GAACGGGAAG AGCCGGACGG ACAGGCAGTG CGTAAGAAAG CGCTGACGGA 351 AGAGCGTGAA CAAACCGTCA GGGAAAAAGC GCAGAAGAAA GATGCCGAAA 401 CGGTTAAAAw ACAAGCGGTA AAACCGTCTA AAGAAACAGA GAAAAAAGCT 451 TCAAAAGAAG AGAAAAAGGC GGCGAAGGAA AAAGTTGCAC CCAAACCAAC 501 CCCGGAACAA ATCCTCAACA GCGGCAGCAT CGAAAAAGCG CGCAGTGCCG 551 CCGCCAAAGA AGTGCAGAAA ATGAAAACGC CGACAAGGCG GAAGCAACGC 601 ATTATCTGCA AATGGGCGCG TATGCCGACC GTCAGAGCGC GGAAGGGCAG 651 CGTGCCAAAC TGGCAATCTT GGGCATATCT TCCAAGGTGG TCGGTTATCA 701 GGCGGGACAT AAAACGCTTT ACCGGGTGCA AAGCGGCAAT ATGTCTGCCG 751 ATGCGGTGA This corresponds to the amino acid sequence <SEQ ID 1268; ORF 306>: m306.pep (partial) 1 ..GLFFGLILAT VIIAGILFYL NQSGQNAFKI PASSKQPAET EILKPXNQXK 51 EDIQPXPADQ NALSEPDAAT EAEQSDAEXA ADKQPVADKA DEVEEKAGEP 101 EREEPDGQAV RKKALTEERE QTVREKAQKK DAETVKXQAV KPSKETEKKA 151 SKEEKKAAKE KVAPKPTPEQ ILNSGSIEKA RSAAAKEVQK MKTPTRRKQR 201 IICKWARMPT VRARKGSVPN WQSWAYLPRW SVIRRDIKRF TGCKAAICLP 251 MR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 306 shows 88.9% identity over a 253 aa overlap with a predicted ORF (ORF 306.ng) from N. gonorrhoeae: m306/g306 10 20 30 40 m306.pep GLFFGLILATVIIAGILFYLΝQSGQΝAFKIPASSKQPAETEILKPX MIIMMIM g306 MFMΝKFSQSGKGLSGFFFGLILATVIIAGILLYLΝQGGQΝAFKIPAPSKQPAETEILKLK 10 20 30 40 50 60 50 60 70 80 90 100 m306.pep ΝQXKEDIQPXPADQΝALSEPDAATEAEQSDAEXAADKQPVADKADEVEEKAGEPEREEPD II IMMMIMMI I II II II II II I II II II II II I II II g306 ΝQPKEDIQPEPADQΝALSEPDVAKEAEQSDAEKAADKQPVADKADEVEEKAGEPEREEPD 70 80 90 100 110 120 110 120 130 140 150 160 m306. pep GQAVRKKALTEEREQTVREKAQKKDAETVKXQAVKPSKETEKKASKEEKKAAKEKVAPKP M 1111 II II I II 11 II I II II 11 II II 11 MMMMMMMMMMMMIMM g306 GQAVRKKALTEEREQTVREKAQKKDAETVKKKAVKPSKETEKKASKEEKKAAKEKVAPKP 130 140 150 160 170 180 170 180 190 200 210 220 m306.pep TPEQILΝSGSIEKARSAAAKEVQKMKTPTRR-KQRIICKWARMPTVRARKGSVPΝWQSWA MMMMMMMIM: : MMMMMIh g306 TPEQILΝSRSIEKARSAAAKEVQKMKΝFGQGGSQRIICKWARMPΝPGARKGSVPΝWQSWA 190 200 210 220 230 240 230 240 250 m306. pep YLPRWSVIRRDIKRFTGCKAAICLPMRX llll g306 YLPKWSAIRRDIKRFTACKAAICPPMRX 250 260 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1269>: a306. seq 1 ATGTTTATGA ACAAATTTTC CCAATCCGGA AAAGGTCTGT CCGGTTTTTT 51 CTTCGGTTTG ATACTGGCGA CGGTCATTAT TGCCGGTATT TTGTTTTATC 101 TGAACCAGAG CGGTCAAAAT GCGTTCAAAA TCCCGGTTCC GTCGAAGCAG 151 CCTGCAGAAA CGGAAATCCT GAAACCGAAA AACCAGCCTA AGGAAGACAT 201 CCAACCTGAA CCGGCCGATC AAAACGCCTT GTCCGAACCG GATGCTGCGA 251 AAGAGGCAGA GCAGTCGGAT GCGGAAAAAG CTGCCGACAA GCAGCCCGTT 301 GCCGACAAAG CCGACGAGGT TGAGGAAAAG GCGGACGAGC CGGAGCGGGA 351 AAAGTCGGAC GGACAGGCAG TGCGCAAGAA AGCACTGACG GAAGAGCGTG 401 AACAAACCGT CGGGGAAAAA GCGCAGAAGA AAGATGCCGA AACGGTTAAA 451 AAACAAGCGG TAAAACCATC TAAAGAAACA GAGAAAAAAG CTTCAAAAGA 501 AGAGAAAAAG GCGGAGAAGG AAAAAGTTGC ACCCAAACCG ACCCCGGAAC 551 AAATCCTCAA CAGCGGCAGC ATCGAAAAAG CGCGCAGTGC CGCTGCCAAA 601 GAAGTGCAGA AAATGAAAAC GCCGACAAGG CGGAAGCAAC GCATTATCTG 651 CAAATGGGCG CGTATGCCGA CCGCCGGAGC GCGGAAGGGC AGCGTGCCAA 701 ACTGGCAATC TTGGGCATAT CTTCCAAGGT GGTCGGTTAT CAGGCGGGAC 751 ATAAAACGCT TTACCGGGTG CAAAGCGGCA ATATGTCTGC CGATGCGGTG 801 A This corresponds to the amino acid sequence <SEQ ID 1270; ORF 306. a>: a306.pep 1 MFMNKFSQSG KGLSGFFFGL ILATVIIAGI LFYLNQSGQN AFKIPVPSKQ 51 PAETEILKPK NQPKEDIQPE PADQNALSEP DAAKEAEQSD AEKAADKQPV 101 ADKADEVEEK ADEPEREKSD GQAVRKKALT EEREQTVGEK AQKKDAETVK 151 KQAVKPSKET EKKASKEEKK AEKEKVAPKP TPEQILNSGS IEKARSAAAK 201 EVQKMKTPTR RKQRIICKWA RMPTAGARKG SVPNWQSWAY LPRWSVIRRD 251 IKRFTGCKAA ICLPMR* m306/a306 93.7% identity in 252 aa overlap 10 20 30 40 m306.pep GLFFGLILATVIIAGILFYLNQSGQNAFKIPASSKQPAETEILKPX I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I a306 MFMNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPVPSKQPAETEILKPK 10 20 30 40 50 60 50 60 70 80 90 100 m306. pep NQXKEDIQPXPADQNALSEPDAATEAEQSDAEXAADKQPVADKADEVEEKAGEPEREEPD II llllll lllllllllllll llllllll I I I I I I I I I I I I I I I I I I Mill: I a306 NQPKEDIQPEPADQNALSEPDAAKEAEQSDAEKAADKQPVADKADEVEEKADEPEREKSD 70 80 90 100 110 120 110 120 130 140 150 160 m306. pep GQAVRKKALTEEREQTVREKAQKKDAETVKXQAVKPSKETEKKASKEEKKAAKEKVAPKP lllllllllllllllll I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I llllllll a306 GQAVRKKALTEEREQTVGEKAQKKDAETVKKQAVKPSKETEKKASKEEKKAEKEKVAPKP 130 140 150 160 170 180 170 180 190 200 210 220 m306.pep TPEQILNSGSIEKARSAAAKEVQKMKTPTRRKQRIICKWARMPTVRARKGSVPNWQSWAY I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : llllllllllllll a306 TPEQILNSGSIEKARSAAAKEVQKMKTPTRRKQRIICKWARMPTAGARKGSVPNWQSWAY 190 200 210 220 230 240 230 240 250 m306.pep LPRWSVIRRDIKRFTGCKAAICLPMRX I I I I I I I I I I I I I I I I I I I I I I I I I I I a306 LPRWSVIRRDIKRFTGCKAAICLPMRX 250 260 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1271>: g307.seq 1 atgaaaacct tcttcaaaac cctttcgacc gcgtcactcg cgctcatcct 51 cgcagcctgc ggcggtcaaa aagacagcgc gcccgcagcc tctgccgccg 101 ccccttctgc cgataacggc gcggcgaaaa aagaaatcgt cttcggcacg 151 accgtgggcg acttcggcga tatggtcaaa gaacaaatcc aagccgagct 201 ggagaaaaaa ggctacaccg tcaaattggt cgaatttacc gactatgtgc 251 gcccgaatct ggcattggcg gagggcgagt tggacatcaa cgtcttccaa 301 cacaaaccct atcttgacga tttcaaaaaa gaacacaacc tggacatcac 351 cgaagccttc caagtgccga ccgcgccttt gggactgtat ccgggcaaac 401 tgaaatcgct ggaagaagtc aaagacggca gcaccgtatc cgcgcccaac 451 gacccgtcca acttcgcacg cgccttggtg atgctgaacg aactgggttg 501 gatcaaactc aaagacggca tcaatccgct gaccgcatcc aaagccgaca 551 tcgcggaaaa cctgaaaaac atcaaaatcg tcgagcttga agccgcacaa 601 ctgccgcgca gccgcgccga cgtggatttt gccgtcgtca acggcaacta 651 cgccataagc agcggcatga agctgaccga agccctgttc caagagccga 701 gctttgccta tgtcaactgg tctgccgtca aaaccgccga caaagacagc 751 caatggctta aagacgtaac cgaggcctat aactccgacg cgttcaaagc 801 ctacgcgcac aaacgcttcg agggctacaa ataccctgcc gcatggaatg 851 aaggcgcagc caaataa This corresponds to the amino acid sequence <SEQ ID 1272; ORF 307.ng>: g307.pep 1 MKTFFKTLST ASLALILAAC GGQKDSAPAA SAAAPSADNG AAKKEIVFGT 51 TVGDFGDMVK EQIQAELEKK GYTVKLVEFT DYVRPNLALA EGELDINVFQ 101 HKPYLDDFKK EHNLDITEAF QVPTAPLGLY PGKLKSLEEV KDGSTVSAPN 151 DPSNFARALV MLNELGWIKL KDGINPLTAS KADIAENLKN IKIVELEAAQ 201 LPRSRADVDF AWNGNYAIS SGMKLTEALF QEPSFAYVNW SAVKTADKDS 251 QWLKDVTEAY NSDAFKAYAH KRFEGYKYPA AWNEGAAK* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1273>: m307.seq (partial) 1 .. CAATGGCTTA AAGACGTAAC CGAGGCCTAT AACTCCGACG CGTTCAAAGC 51 CTACGCGCAC AAACGCTTCG AGGGCTACAA ATCCCCTGCC GCATGGAATG 101 AAGGCGCAGC CAAATAA This corresponds to the amino acid sequence <SEQ ID 1274; ORF 307>: m307.pep (partial) 1 ..QWLKDVTEAY NSDAFKAYAH KRFEGYKSPA AWNEGAAK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 307 shows 97.4% identity over a 38 aa overlap with a predicted ORF (ORF 307.ng) from N gonorrhoeae: m307/g307 10 20 30 m307.pep QWLKDVTEAYΝSDAFKAYAHKRFEGYKSPA M M I M M M M M M M M M M M I I g307 SGMKLTEALFQEPSFAYVΝWSAVKTADKDSQWLKDVTEAYΝSDAFKAYAHKRFEGYKYPA 230 240 250 260 270 280 39 m307. pep AWΝEGAAKX 1 1 1 1 E 1 1 1 1 g307 AWΝEGAAKX The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1275>: a307. seq 1 ATGAAAACCT TCTTCAAAAC CCTTTCCGCC GCCGCACTCG CGCTCATCCT 51 CGCCGCCTGC GGCGGTCAAA AAGATAGCGC GCCCGCCGCA TCCGCTTCTG 101 CCGCCGCCGA CAACGGCGCG GCGAAAAAAG ΝAATCGTCTT CGGCACGACC 151 GTCGGCGACT TCGGCGATAT GGTCAAAGAA CAAATCCAAC CCGAGCTGGA 201 GAAAAAAGGC TACACCGTCA AACTGGTCGA GTTTACCGAC TATGTGCGCC 251 CGAATCTGGC ATTGGCTGAG GGCGAGTΝGG ACATCAACGT CTTCCAACAC 301 AAACCCTATC TTGACGACTT CAAAAAAGAA CACAATCTGG ACATCACCGA 351 AGTCTTCCAA GTGCCGACCG CGCCTTTGGG ACTGTACCCG GGCAAGCTGA 401 AATCGCTGGA AGAAGTCAAA GACGGCAGCA CCGTATCCGC GCCCAACGAC 451 CCGTCCAACT TCGCCCGCGT CTTGGTGATG CTCGACGAAC TGGGTTGGAT 501 CAAACTCAAA GAΝGGCATCA ATCCGCTGAC CGCATCCAAA GCGGACATTG 551 CCGAAAACCT GAAAAACATC AAAATCGTCG AGCTTGAAGC CGCGCAACTG 601 CCGCGTAGCC GCGCCGACGT GGATTTTGΝC GTCGTCAACG GCAAΝTACGC 651 CATAAGCAGC GGCATGAAGC TGACCGAAGC CCTGTTCCAA GAACCGAGCT 701 TTGCCTATGT CAACTGGTCT GCCGTCAAAA CCGCCGACAA AGACAGCCAA 751 TGGCTTAAAG ACGTAACCGA GGCCTATAAC TCCGACGCGT TCAAAGCCTA 801 CGCGCACAAA CGCTTCGAGG GCTACAAATC CCCTGCCGCA TGGAATGAAG 851 GCGCAGCCAA ATAA This corresponds to the amino acid sequence <SEQ ID 1276; ORF 307.a>: a307.pep 1 MKTFFKTLSA AALALILAAC GGQKDSAPAA SASAAADΝGA AKKXIVFGTT 51 VGDFGDMVKE QIQPELEKKG YTVKLVEFTD YVRPNLALAE GEXDINVFQH 101 KPYLDDFKKE HNLDITEVFQ VPTAPLGLYP GKLKSLEEVK DGSTVSAPND 151 PSNFARVLVM LDELGWIKLK XGINPLTASK ADIAENLKNI KIVELEAAQL 201 PRSRADVDFX WNGXYAISS GMKLTEALFQ EPSFAYVNWS AVKTADKDSQ 251 WLKDVTEAYN SDAFKAYAHK RFEGYKSPAA WNEGAAK* m307/a307 100.0% identity in 38 aa overlap 10 20 30 m307.pep QWLKDVTEAYNSDAFKAYAHKRFEGYKSPA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a307 SGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYKSPA 220 230 240 250 260 270 39 m307. pep AWNEGAAKX a307 AWNEGAAKX 280 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1277>: g308.seq 1 ATGTTAAATC GGGTATTTTA TCGGATATTG GGTGTTGCCG ACAATTTGTA 51 TCCGTGTTTA TCGGATTTCT GTTTTTTCAC TATAATAGCC GGTTTGCCGT 101 TGCAGGCGGT TTTATGGGAA AGGCGGATGA TGGTACGGCG TTTGATAATC 151 GGCATCAGCG GGGCGAGCGG TTTCCAATAC GGCGTGAAGG CTTTGGAACT 201 TTTGCGCGCG CAAGATGTCG AAACGCACCT TGTGGTATCG AAAGGCGCGG 251 AGATGGCGCG CGCTTCGGAA ACGGATTATA CGAAAGACGA AGTATATGCC 301 TTGGCTGATT TCGTCCATCC GATCGGCAAT ATCGGGGCGT GCATTGCCAG 351 CGGTACGTTT AAAACGGACG GGATGCTGGT CGCACCCTGT TCGATGCGGA 401 CGCTTGCCTC TGTCGCGCAC GGCTTCGGCG ACAACCTCTT GACGCGTGCG 451 GCGGATGTGG TTTTGAAGGA AAGGCGGCGG CTGGTGCTGA TGGTGCGCGA 501 AACGCCGCTG AACCTTGCCC ATTTGGACAA TATGAAGCGG GTAACGGAAA 551 TGGGCGGCGT GGTGTTTCCC CCTGTTCCTG CGATGTACCG CAAGCCGCAG 601 ACGGCGGACG ACATAGTGGC GCACAGTATC GCACACACGC TGTCGCTGTT 651 CGGAATCGAT ACGCCGGATT TGGCGGAATG GCAGGGAATG GCGGATTAA This corresponds to the amino acid sequence <SEQ ID 1278; ORF 308.ng>: g308.pep 1 MLΝRVFYRIL GVADΝLYPCL SDFCFFTIIA GLPLQAVLWE RRMMVRRLII 51 GISGASGFQY GVKALELLRA QDVETHL S KGAEMARASE TDYTKDEVYA 101 LADFVHPIGΝ IGACIASGTF KTDGMLVAPC SMRTLASVAH GFGDΝLLTRA 151 ADWLKERRR LVLMVRETPL ΝLAHLDΝMKR VTEMGGWFP PVPAMYRKPQ 201 TADDIVAHSI AHTLSLFGID TPDLAEWQGM AD* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1279>: m308.seq (partial) 1 ATGTTAAATC GGGTATTTTA TCGGATATTG GGTGTTGCCG ACAATTTGTA 51 TCCGCGTTTA TCGGATTTCT GTTTTTTCAC TATAATAGCC GGTTTGCCGT 101 TGCAGGCGGT TTTATGGGAA AGGCGGATGA TGGTACGGCG TTTGATAATC 151 GGCATCAGCG GGGCGAGCGG TTTCCAATAC GGCGTGAAGG CTTTGGAACT 201 TTTGCGCGCG CAAGATGTCG AAACGCACCT TGTGGTATCG AAAGGTGCGG 251 AGATGGCGCG CGCTTCGGAA ACGGCTTATG CGAGAGACGA GGTATATGCC 301 TTGGCGGACT TCGTGCATCC GATCGGCAAT ATCGGGGCGT GCATTGCCAG 351 CGGTACGTTT AAAACGGATG GGATGCTGGT CGCCCCCTGT TCGATGCGGA 401 CGCTTGCCTC TGTCGCGCAC GGCTTCGGCG ACAATCTGcT GACGCgTGCG 451 GCGGATGTGG TTTTGAAGGA AAGGCGGCGG CTGGTGCTGA TGGTGCGCGA 501 AACGCCGCTG AACCTTGCCC ATTTGGACAA TATGAAGCGG GwAACGGAAA 551 TGGGCGGCGT GGTGTTTCCC CCTGTTCCTG CGATGTACCG CAAACCGCAG 601 ACGGCGGACG ACATAGTGGC GCACAGTGTT GCACACGCtT TGTCGCTGTT 651 CGGAATCGAT ACGCCGGATT CGGCGGAATG GCArGGAATG gcG... This corresponds to the amino acid sequence <SEQ ID 1280; ORF 308>: m308 . pep (partial ) 1 MLNRVFYRIL GVADNLYPRL SDFCFFTIIA GLPLQAVLWE RRMMVRRLII 51 GISGASGFQY GVKALELLRA QDVETHLWS KGAEMARASE TAYARDEVYA 101 LADFVHPIGN IGACIASGTF KTDGMLVAPC SMRTLASVAH GFGDNLLTRA 151 ADWLKERRR LVLMVRETPL NLAHLDNMKR XTEMGG FP PVPAMYRKPQ 201 TADDIVAHSV AHALSLFGID TPDSAEWQGM A.. Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 308 shows 96.5% identity over a 231 aa overlap with a predicted ORF (ORF 308.ng) from N gonorrhoeae: m308/g308 10 20 30 40 50 60 m308.pep MLΝRVFYRILGVADΝLYPRLSDFCFFTIIAGLPLQAVLWERRMMVRRLIIGISGASGFQY 11111111111 ! 1 i 11 i i 1111111111 M ! 111111111 E 1111 ! ! M ! I i 111111 ! g308 MLΝRVFYRILGVADΝLYPCLSDFCFFTIIAGLPLQAVLWERRMMVRRLIIGISGASGFQY 10 20 30 40 50 60 70 80 90 100 110 120 m308. pep GVKALELLRAQDVETHLWSKGAEMARASETAYARDEVYALADFVHPIGΝIGACIASGTF M M M M M M M M M M M I M M M M h M I I I I I I I I I I I I I I I 1 1 I I I I I I I g308 GVKALELLRAQDVETHLWSKGAEMARASETDYTKDEVYALADFVHPIGΝIGACIASGTF 70 80 90 100 110 120 130 140 150 160 170 180 m308. pep KTDGMLVAPCSMRTLASVAHGFGDΝLLTRAADWLKERRRLVLMVRETPLΝLAHLDΝMKR MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g308 KTDGMLVAPCSMRTLASVAHGFGDΝLLTRAADWLKERRRLVLMVRETPLΝLAHLDΝMKR 130 140 150 160 170 180 190 200 210 220 230 m308 . ep XTEMGGWFPPVPAMYRKPQTADDIVAHSVAHALSLFGIDTPDSAEWQGMA ! ! I M 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 i 1 1 I I I = 1 1 = 1 1 1 I I 1 1 1 1 1 l l l l l l l g308 VTEMGGWFPPVPAMYRKPQTADDIVAHS IAHTLSLFGIDTPDLAEWQGMADX 190 200 210 220 230 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1281>: a308. seq 1 ATGTTAAATC GGATATTTTA TCGGATATTG GGTGTTGCCG ACAATTTGTA 51 TCCGTATTTA TCGGATTTCT GTTTTTTCAC TATAATAGCC GGTTTGCCGT 101 TGCAGGCGGT TTTATGGGAA AGGCGGATGA TGGTACGGCG TTTGATAATC 151 GGCATCAGTG GGGCGAGCGG TTTCCAATAC GGCGTGAAGG CTTTGGAΝCT 201 TTTACGCGCG CAAGATATCG AAACGCACCT TGTGGTATCG AAAGGTGCGG 251 AGATGGCGCG CGCTTCGGAA ACGGΝTTATG CGAGAGACGA ΝGTATATGCC 301 TTGGCGGACT TΝGTGCATCC GATCGGCAAT ATCGGGGCGT GCATTGCCAG 351 CGGTACGTTT AAAACGGACG GGATGCTGGT CGCCCCCTGT TCGATGCGGA 401 CGCTTGCCTC GGTCGTGCAC GGCTTCGGCG ACAACCTCTT GACGCGTGCG 451 GCGGATGTGG TTTTGAAGGA AAGGCGGCGG CTGGTGCTGA TGGTGCGCGA 501 AACGCCGCTG AACCTTGCCC ATTTGGACAA TATGAAΝCGG GTAACGGAAA 551 TGGGCGGCGT GGTGTTTCCC CCTGTTCCTG CGATGTACCG CAAACCGCAG 601 ACGGCGGACG ACATAGTGGC GCACAGTGTT GCACACGCTT TGTCGCTGTT 651 CGGAATCGAT ACGCCGGATT CGGCGGAATG GCAGGGAATG GCGGATTAA This corresponds to the amino acid sequence <SEQ ID 1282; ORF 308.a>: a308.pep 1 MLΝRIFYRIL GVADΝLYPYL SDFCFFTIIA GLPLQAVLWE RRMMVRRLII 51 GISGASGFQY GVKALXLLRA QDIETHLVVS KGAEMARASE TXYARDXVYA 101 LADXVHPIGΝ IGACIASGTF KTDGMLVAPC SMRTLASVVH GFGDΝLLTRA 151 ADWLKERRR LVLMVRETPL ΝLAHLDΝMXR VTEMGGWFP PVPAMYRKPQ 201 TADDIVAHSV AHALSLFGID TPDSAEWQGM AD* m308/a308 95.7% identity in 231 aa overlap 10 20 30 40 50 60 m308.pep MLNRVFYRILGVADNLYPRLSDFCFFTIIAGLPLQAVLWERRMMVRRLIIGISGASGFQY I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a308 MLNRIFYRILGVADNLYPYLSDFCFFTIIAGLPLQAVLWERRMMVRRLIIGISGASGFQY 10 20 30 40 50 60 70 80 90 100 110 120 m308.pep GVKALELLRAQDVETHLVVSKGAEMARASETAYARDEVYALADFVHPIGNIGACIASGTF I I I I I I I I I I I :| I I I I I I I I I I I I I I I I I llll I I I I I I I I I I I I I I I I a308 GVKALXLLRAQDIETHLWSKGAEMARASETXYARDXVYALADXVHPIGNIGACIASGTF 70 80 90 100 110 120 130 140 150 160 170 180 m308 . pep KTDGMLVAPCSMRTLASVAHGFGDNLLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKR I I I I I I I I I I I I I I I I I h I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a308 KTDGMLVAPCSMRTLASVVHGFGDNLLTRAADVVLKERRRLVLMVRETPLNLAHLDNMXR 130 140 150 160 170 180 190 200 210 220 230 m308.pep XTEMGGVVFPPVPAMYRKPQTADDIVAHSVAHALSLFGIDTPDSAEWQGMA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a308 VTEMGGVVFPPVPAMYRKPQTADDIVAHSVAHALSLFGIDTPDSAEWQGMADX 190 200 210 220 230 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1283>: g308-l.seq 1 ATGTTAAATC GGGTATTTTA TCGGATATTG GGTGTTGCCG ACAATTTGTA 51 TCCGTGTTTA TCGGATTTCT GTTTTTTCAC TATAATAGCC GGTTTGCCGT 101 TGCAGGCGGT TTTATGGGAA AGGCGGATGA TGGTACGGCG TTTGATAATC 151 GGCATCAGCG GGGCGAGCGG TTTCCAATAC GGCGTGAAGG CTTTGGAACT 201 TTTGCGCGCG CAAGATGTCG AAACGCACCT TGTGGTATCG AAAGGCGCGG 251 AGATGGCGCG CGCTTCGGAA ACGGATTATA CGAAAGACGA AGTATATGCC 301 TTGGCTGATT TCGTCCATCC GATCGGCAAT ATCGGGGCGT GCATTGCCAG 351 CGGTACGTTT AAAACGGACG GGATGCTGGT CGCACCCTGT TCGATGCGGA 401 CGCTTGCCTC TGTCGCGCAC GGCTTCGGCG ACAACCTCTT GACGCGTGCG 451 GCGGATGTGG TTTTGAAGGA AAGGCGGCGG CTGGTGCTGA TGGTGCGCGA 501 AACGCCGCTG AACCTTGCCC ATTTGGACAA TATGAAGCGG GTAACGGAAA 551 TGGGCGGCGT GGTGTTTCCC CCTGTTCCTG CGATGTACCG CAAGCCGCAG 601 ACGGCGGACG ACATAGTGGC GCACAGTATC GCACACACGC TGTCGCTGTT 651 CGGAATCGAT ACGCCGGATT TGGCGGAATG GCAGGGAATG GCGGATTAA This corresponds to the amino acid sequence <SEQ ID 1284; ORF 308-1. ng>: g308-l.pep 1 MLΝRVFYRIL GVADΝLYPCL SDFCFFTIIA GLPLQAVLWE RRMMVRRLII 51 GISGASGFQY GVKALELLRA QDVETHLWS KGAEMARASE TDYTKDEVYA 101 LADFVHPIGΝ IGACIASGTF KTDGMLVAPC SMRTLASVAH GFGDΝLLTRA 151 ADWLKERRR LVLMVRETPL ΝLAHLDΝMKR VTEMGGWFP PVPAMYRKPQ 201 TADDIVAHSI AHTLSLFGID TPDLAEWQGM AD* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1285>: m308-l.seq 1 ATGTTAAATC GGGTATTTTA TCGGATATTG GGTGTTGCCG ACAATTTGTA 51 TCCGCGTTTA TCGGATTTCT GTTTTTTCAC TATAATAGCC GGTTTGCCGT 101 TGCAGGCGGT TTTATGGGAA AGGCGGATGA TGGTACGGCG TTTGATAATC 151 GGCATCAGCG GGGCGAGCGG TTTCCAATAC GGCGTGAAGG CTTTGGAACT 201 TTTGCGCGCG CAAGATGTCG AAACGCACCT TGTGGTATCG AAAGGTGCGG 251 AGATGGCGCG CGCTTCGGAA ACGGCTTATG CGAGAGACGA GGTATATGCC 301 TTGGCGGACT TCGTGCATCC GATCGGCAAT ATCGGGGCGT GCATTGCCAG 351 CGGTACGTTT AAAACGGATG GGATGCTGGT CGCCCCCTGT TCGATGCGGA 401 CGCTTGCCTC TGTCGCGCAC GGCTTCGGCG ACAATCTGCT GACGCGTGCG 451 GCGGATGTGG TTTTGAAGGA AAGGCGGCGG CTGGTGCTGA TGGTGCGCGA 501 AACGCCGCTG AACCTTGCCC ATTTGGACAA TATGAAGCGG GTAACGGAAA 551 TGGGCGGCGT GGTGTTTCCC CCTGTTCCTG CGATGTACCG CAAACCGCAG 601 ACGGCGGACG ACATAGTGGC GCACAGTGTT GCACACGCTT TGTCGCTGTT 651 CGGAATCGAT ACGCCGGATT CGGCGGAATG GCAGGGAATG GCGGATTAA This corresponds to the amino acid sequence <SEQ ID 1286; ORF 308- 1>: m308-l.pep 1 MLΝRVFYRIL GVADNLYPRL SDFCFFTIIA GLPLQAVLWE RRMMVRRLII 51 GISGASGFQY GVKALELLRA QDVETHLWS KGAEMARASE TAYARDEVYA 101 LADFVHPIGN IGACIASGTF KTDGMLVAPC SMRTLASVAH GFGDNLLTRA 151 ADWLKERRR LVLMVRETPL NLAHLDNMKR VTEMGGWFP PVPAMYRKPQ 201 TADDIVAHSV AHALSLFGID TPDSAEWQGM AD* m308-l/g308-l 97.0% identity in 232 aa overlap 10 20 30 40 50 60 m308-l.pep MLNRVFYRILGVADNLYPRLSDFCFFTIIAGLPLQAVLWERRMMVRRLIIGIΞGASGFQY I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I g308-l MLNRVFYRILGVADNLYPCLSDFCFFTIIAGLPLQAVLWERRMMVRRLIIGISGASGFQY 10 20 30 40 50 60 70 80 90 100 110 120 m308-1. pep GVKALELLRAQDVETHLWSKGAEMARASETAYARDEVYALADFVHPIGNIGACIASGTF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : : I I I I I I I I I I I I I I I I I I I I I I I I I g308- l GVKALELLRAQDVETHLWSKGAEMARASETDYTKDEVYALADFVHPIGNIGACIASGTF 70 80 90 100 110 120 130 140 150 160 170 180 m308 - 1 . pep KTDGMLVAPCSMRTLASVAHGFGDNLLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g308-1 KTDGMLVAPCSMRTLASVAHGFGDNLLTRAADWLKERRRLVLMVRETPLNLAHLDNMKR 130 140 150 160 170 180 190 200 210 220 230 m308-l .pep VTEMGGWFPPVPAMYRKPQTADDIVAHSVAHALSLFGIDTPDSAEWQGMADX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I : I I I I I I I I I I lllllllll g308-l VTEMGGWFPPVPAMYRKPQTADDIVAHSIAHTLSLFGIDTPDLAEWQGMADX 190 200 210 220 230 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1287>: a308-l.seq 1 ATGTTAAATC GGATATTTTA TCGGATATTG GGTGTTGCCG ACAATTTGTA 51 TCCGTATTTA TCGGATTTCT GTTTTTTCAC TATAATAGCC GGTTTGCCGT 101 TGCAGGCGGT TTTATGGGAA AGGCGGATGA TGGTACGGCG TTTGATAATC 151 GGCATCAGTG GGGCGAGCGG TTTCCAATAC GGCGTGAAGG CTTTGGAΝCT 201 TTTACGCGCG CAAGATATCG AAACGCACCT TGTGGTATCG AAAGGTGCGG 251 AGATGGCGCG CGCTTCGGAA ACGGΝTTATG CGAGAGACGA ΝGTATATGCC 301 TTGGCGGACT TΝGTGCATCC GATCGGCAAT ATCGGGGCGT GCATTGCCAG 351 CGGTACGTTT AAAACGGACG GGATGCTGGT CGCCCCCTGT TCGATGCGGA 401 CGCTTGCCTC GGTCGTGCAC GGCTTCGGCG ACAACCTCTT GACGCGTGCG 451 GCGGATGTGG TTTTGAAGGA AAGGCGGCGG CTGGTGCTGA TGGTGCGCGA 501 AACGCCGCTG AACCTTGCCC ATTTGGACAA TATGAAΝCGG GTAACGGAAA 551 TGGGCGGCGT GGTGTTTCCC CCTGTTCCTG CGATGTACCG CAAACCGCAG 601 ACGGCGGACG ACATAGTGGC GCACAGTGTT GCACACGCTT TGTCGCTGTT 651 CGGAATCGAT ACGCCGGATT CGGCGGAATG GCAGGGAATG GCGGATTAA This corresponds to the amino acid sequence <SEQ ID 1288; ORF 308-1. a>: a308-l.pep 1 MLΝRIFYRIL GVADΝLYPYL SDFCFFTIIA GLPLQAVLWE RRMMVRRLII 51 GISGASGFQY GVKALXLLRA QDIETHLVVΞ KGAEMARASE TXYARDXVYA 101 LADXVHPIGΝ IGACIASGTF KTDGMLVAPC ΞMRTLAΞVVH GFGDΝLLTRA 151 ADWLKERRR LVLMVRETPL ΝLAHLDΝMXR VTEMGGWFP PVPAMYRKPQ 201 TADDIVAHSV AHALSLFGID TPDSAEWQGM AD* a308-l/m308-l 96.1% identity in 232 aa overlap 10 20 30 40 50 60 a308-l MLΝRIFYRILGVADΝLYPYLSDFCFFTIIAGLPLQAVLWERRMMVRRLIIGISGASGFQY I I I h I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m308-l MLNRVFYRILGVADNLYPRLSDFCFFTIIAGLPLQAVLWERRMMVRRLIIGISGASGFQY 10 20 30 40 50 60 70 80 90 100 110 120 a308-l GVKALXLLRAQDIETHLVVSKGAEMARASETXYARDXVYALADXVHPIGNIGACIASGTF I I I I I I I I I I I : I I I I I I I I I II I I I I I I I llll II I I I I I I I I I I I I I I m308-l GVKALELLRAQDVETHLVVSKGAEMARASETAYARDEVYALADFVHPIGNIGACIASGTF 70 80 90 100 110 120 130 140 150 160 170 180 a308-l KTDGMLVAPCSMRTLASWHGFGDNLLTRAADWLKERRRLVLMVRETPLNLAHLDNMXR I I I I I I I I I II I I I I I I hi I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M I I I I m308- l KTDGMLVAPCSMRTLASVAHGFGDNLLTRAADWLKERRRLVLMVRETPLNLAHLDNMKR 130 140 150 160 170 180 190 200 210 220 230 a308-l VTEMGGWFPPVPAMYRKPQTADDIVAHSVAHALSLFGIDTPDSAEWQGMADX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m308-1 VTEMGGVVFPPVPAMYRKPQTADDIVAHSVAHALSLFGIDTPDΞAEWQGMADX 190 200 210 220 230 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1289>: g311. seq 1 atgttcagtt tcggctgggc gtttgaccgc ccgcagtatg agttgggttc 51 gctgtcgcct gttgcggcac ttgcgtgccg gcgcgctttg gggtgtttgg 101 gtttggaaac gcaaatcaag tggccaaacg atttggtcgt cggacgcgac 151 aaattgggcg gcattctgat tgaaacagtc agggcgggcg gtaaaacggt 201 tgccgtggtc ggtatcggca tcaatttcgt gctgcccaag gaagtggaaa 251 acgccgcttc cgtgcagtcg ctgtttcaga cggcatcgcg gcggggcaat 301 gccgatgccg ccgtattgct ggaaacattg cttgcggaac tgggcgcggt 351 gttggaacaa tatgcggaag aagggttcgc gccattttta aatgagtatg 401 aaacggccaa ccgcgaccac ggcaaggcgg tattgctgtt gcgcgacggc 451 gaaaccgtgt gcgaaggcac ggttaaaggc gtggacggac gaggcgttct 501 gcacttggaa acggcagaag gcgaacagac ggtcgtcagc ggcgaaatca 551 gcctgcggcc cgacaacagg tcggtttccg tgccgaagcg gccggattcg 601 gaacgttttt tgctgttgga aggcgggaac agccggctca agtgggcgtg 651 ggtggaaaac ggcacgttcg caaccgtggg cagcgcgccg taccgcgatt 701 tgtcgccttt gggcgcggag tgggcggaaa aggcggatgg aaatgtccgc 751 atcgtcggtt gcgccgtgtg cggagaatcc aaaaaggcac aagtgaagga 801 acagctcgcc cgaaaaatcg agtggctgcc gtcttccgca caggctttgg 851 gcatacgcaa ccactaccgc caccccgaag aacacggttc cgaccgttgg 901 ttcaacgcct tgggcagccg ccgcttcagc cgcaacgcct gcgtcgtcgt 951 cagttgcggc acggcggtaa cggttgacgc gctcaccgat gacggacatt 1001 atctcggcgg aaccatcatg cccggcttcc acctgatgaa agaatcgctc 1051 gccgtccgaa ccgccaacct caaccgcccc gccggcaaac gttacccttt 1101 cccgaccaca acgggcaacg ccgtcgcaag cggcatgatg gacgcggttt 1151 gcggctcgat aatgatgatg cacggccgtt tgaaagaaaa aaacggcgcg 1201 ggcaagcctg tcgatgtcat cattaccggc ggcggcgcgg cgaaagtcgc 1251 cgaagccctg ccgcctgcat ttttggcgga aaataccgtg cgcgtggcgg 1301 acaacctcgt catccacggg ctgctgaacc tgattgccgc cgaaggcggg 1351 gaatcggaac acgcttaa This corresponds to the amino acid sequence <SEQ ID 1290; ORF 31 l.ng>: g311.pep 1 MFSFGWAFDR PQYELGSLSP VAALACRRAL GCLGLETQIK WPΝDLWGRD 51 KLGGILIETV RAGGKTVAW GIGIΝFVLPK EVEΝAASVQS LFQTASRRGΝ 101 ADAAVLLETL LAELGAVLEQ YAEEGFAPFL ΝEYETAΝRDH GKAVLLLRDG 151 ETVCEGTVKG VDGRGVLHLE TAEGEQTWS GEISLRPDΝR SVSVPKRPDS 201 ERFLLLEGGΝ SRLKWAWVEΝ GTFATVGSAP YRDLSPLGAE WAEKADGΝVR 251 IVGCAVCGES KKAQVKEQLA RKIEWLPSSA QALGIRΝHYR HPEEHGSDRW 301 FΝALGSRRFS RΝACVWSCG TAVTVDALTD DGHYLGGTIM PGFHLMKESL 351 AVRTAΝLΝRP AGKRYPFPTT TGΝAVASGMM DAVCGSIMMM HGRLKEKΝGA 401 GKPVDVIITG GGAAKVAEAL PPAFLAEΝTV RVADΝLVIHG LLΝLIAAEGG 451 ESEHA* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1291>: m311.seq (partial) 1 ATGTTCAGTT TTGGCTGGGT GTTTGACCGG CCGCAGTATG AGTTGGGTTC 51 GCTGTCGCCT GTTGCGGCAG TGGCGTGTCG GCGCGCCTTG TCGCGTTTAG 101 GTTTGGATGT GCArATTAAG TGGCCCAATG ATTTGGTTGT CGGACGCGAC 151 AAATTGGGCG GCATTCTGAT TGAAACGGTC AGGACGGGCG GCAAAACGGT 201 TGCCGTGGTC GGTATCGGCA TCAATTTTGT CCTGCCCAAn GAAGTAGAAA 251 ATGCCGCTTC CGTGCAATCG CTGTTTCAGA CGGCATCGCG GCGGGGCAAT 301 GCCGATGCCG CCGTGCTGCT nnnnnnnnnn nnnnnnnnnn nnnnGGAAAT 351 CAGCCTGCGG TCCGACnACA GGCCGGTTTC CGTGnCGAAG CGGCGGGATT 401 CGGAACGTTT TCTGCTGTTG GACGGCGGCA ACAGCCGGCT CAAGTGGgCG 451 TGGGTGGAAA ACGGCACGTT CGCAACCGTC GGTAGCGCGC CGTACCgCGA 501 TTTGTCGCCT TTGGGCGCGG AGTGGGCGGA AAAGGCGGAT GGAAATGTCC 551 GCATCGTCGG TTGCGCTGTG TGCGGAGAAT TCAAAAAGGC ACAAGTGCAG 601 GAACAGCTCG CCCGAAAAAT CGAGTGGCTG CCGTCTTCCG CACAGGCTTT 651 GTTTGGCATA CGCAACCACT ACCGCCACCC CGAAGAACAC GGTTCCGACC 701 GCTGGTTCAA CGCCTTGGGC AGCCGCCGCT TCAGCCGCAA CGCyTGCGTC 751 GTCGTCAGTT GCGGCACGGC GGTAACGGTT GACGCGCTCA CCGATGACGG 801 ACATTATCTC GGrgGAACCA TCATGCCCGG TTTCCACCTG ATGAAAGAAT 851 CGCTCGCCGT CCGAACCGCC AACCTCAACC GGCACGCCGG TAAGCGTTAT 901 CCTTTCCCGA CCACAACGGG CAATGCCGTC GCCAGCGGCA TGATGGATGC 951 GGTTTGCGGC TCGGTTATGA TGATGCACGG GCGTTTGAAA GAAAAAACCG 1001 GGGCGGGCAA GCCTGTCGAT GTCATCATTA CCGGCGGCGG CGCGGCAAAA 1051 GTTGCCGAAG CCCTGCCGCC TGCATTTTTG GCGGAAAATA CCGTGCGCGT 1101 GGCGGACAAC CTCGTCATTT ACGGGTTGTT GAACATGATT GCCGCCGAAG 1151 GCAGGGAATA TGAACAT.... This corresponds to the amino acid sequence <SEQ ID 1292; ORF 311>: m311.pep (partial) 1 MFSFGWVFDR PQYELGSLSP VAAVACRRAL SRLGLDVQIK WPNDLWGRD 51 KLGGILIETV RTGGKTVAW GIGINFVLPX EVENAASVQS LFQTASRRGN 101 ADAAVLLXXX XXXXXEISLR SDXRPVSVXK RRDSERFLLL DGGNSRLKWA 151 WVENGTFATV GSAPYRDLSP LGAEWAEKAD GNVRIVGCAV CGEFKKAQVQ 201 EQLARKIEWL PSSAQALFGI RNHYRHPEEH GSDRWFNALG SRRFSRNACV 251 WSCGTAVTV DALTDDGHYL GGTIMPGFHL MKESLAVRTA NLNRHAGKRY 301 PFPTTTGNAV ASGMMDAVCG SVMMMHGRLK EKTGAGKPVD VIITGGGAAK 351 VAEALPPAFL AENTVRVADN LVIYGLLNMI AAEGREYEH .... Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 311 shows 78.5% identity over a 455 aa overlap with a predicted ORF (ORF 311.ng) from N gonorrhoeae: m311/g311 10 20 30 40 50 60 m311. pep MFSFGWVFDRPQYELGSLSPVAAVACRRALSRLGLDVQIKWPΝDLWGRDKLGGILIETV 111111 : 111 II 1111111111 i = 111111 = MhMMMMMMMMMIIMM g 311 MFS FGWAFDRPQYELGS LS PVAALACRRALGCLGLETQ I KWPΝDLWGRDKLGGI L I ETV 10 20 30 40 50 60 70 80 90 100 110 m311. pep RTGGKTVAWGIGIΝFVLPXEVEΝAASVQSLFQTASRRGΝADAAVLLXXX g311 RAGGKTVAWGIGIΝFVLPKEVEΝAASVQSLFQTASRRGΝADAAVLLETLLAELGAVLEQ 70 80 90 100 110 120 m311. pep XXXX g311 YAEEGFAPFLΝEYETAΝRDHGKAVLLLRDGETVCEGTVKGVDGRGVLHLETAEGEQTWS 130 140 150 160 170 180 120 130 140 150 160 170 m311. pep XEISLRSDXRPVSVXKRRDSERFLLLDGGΝSRLKWAWVEΝGTFATVGSAPYRDLSPLGAE Mill I I III II MMMIhlMMMMMMIMMMMMMMIMM g311 GEISLRPDΝRSVSVPKRPDSERFLLLEGGΝSRLKWAWVEΝGTFATVGSAPYRDLSPLGAE 190 200 210 220 230 240 180 190 200 210 220 230 m311. pep WAEKADGΝVRIVGCAVCGEFKKAQVQEQLARKIEWLPSSAQALFGIRΝHYRHPEEHGSDR MMIMMMMMMM I II I h II I II I II II II II I II II 1111111 M 11 M I g311 WAEKADGΝVRIVGCAVCGESKKAQVKEQLARKIEWLPSSAQAL-GIRΝHYRHPEEHGSDR 250 260 270 280 290 240 250 260 270 280 290 m311. pep WFNALGSRRFSRNACVWSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNR M M I M M M M M M I M M M M M M M M M M M M M M I I M M M M M M g311 WFNALGSRRFSRNACVWSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNR 300 310 320 330 340 350 300 310 320 330 340 350 m311 . pep HAGKRYPFPTTTGNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEA M M M M I M M M I M I I 1 1 I I I h 1 1 M 1 1 I I I h I I I I I I I I I I I I I I I I I I I I I g311 PAGKRYPFPTTTGNAVASGMMDAVCGSIMMMHGRLKEKNGAGKPVDVIITGGGAAKVAEA 360 370 380 390 400 410 360 370 380 389 m311.pep LPPAFLAENTVRVADNLVIYGLLNMIAAEGREYEH II I ! 111111111111111 : 1111 H I i II I II g311 LPPAFLAENTVRVADNLVIHGLLNLIAAEGGESEHAX 420 430 440 450 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1293>: a311. seq 1 ATGTTCAGTT TTGGCTGGGT GTTTGACCGG CCGCAGTATG AGTTGGGTTC 51 GCTGTCGCCT GTTGCGGCAG TGGCGTGCCG GCGCGCCTTG TCGCGTTTGG 101 GTTTGAAAAC GCAAATCAAG TGGCCAAACG ATTTGGTCGT CGGACGCGAC 151 AAATTGGGCG GCATTCTGAT TGAAACGGTC AGGACGGGCG GCAAAACGGT 201 TGCCGTGGTC GGTATCGGCA TCAATTTCGT GCTGCCCAAG GAAGTGGAAA 251 ACGCCGCTTC CGTGCAATCG CTGTTTCAGA CGGCATCGCG GCGGGGAAAT 301 GCCGATGCCG CCGTGTTGCT GGAAACGCTG TTGGCGGAAC TTGATGCGGT 351 GTTGTTGCAA TATGCGCGGG ACGGATTTGC GCCTTTTGTG GCGGAATATC 401 AGGCTGCCAA CCGCGACCAC GGCAAGGCGG TATTGCTGTT GCGCGACGGC 451 GAAACCGTGT TCGAAGGCAC GGTTAAAGGC GTGGACGGAC AAGGCGTTCT 501 GCACTTGGAA ACGGCAGAGG GCAAACAGAC GGTCGTCAGC GGCGAAATCA 551 GCCTGCGGTC CGACGACAGG CCGGTTTCCG TGCCGAAGCG GCGGGATTCG 601 GAACGTTTTC TGCTGTTGGA CGGCGGCAAC AGCCGGCTCA AGTGGGCGTG 651 GGTGGAAAAC GGCACGTTCG CAACCGTCGG TAGCGCGCCG TACCGCGATT 701 TGTCGCCTTT GGGCGCGGAG TGGGCGGAAA AGGTGGATGG AAATGTCCGC 751 ATCGTCGGTT GCGCCGTGTG CGGAGAATTC AAAAAGGCAC AAGTGCAGGA 801 ACAGCTCGCC CGAAAAATCG AGTGGCTGCC GTCTTCCGCA CAGGCTTTGG 851 GCATACGCAA CCACTACCGC CACCCCGAAG AACACGGTTC CGACCGCTGG 901 TTCAACGCCT TGGGCAGCCG CCGCTTCAGC CGCAACGCCT GCGTCGTCGT 951 CAGTTGCGGC ACGGCGGTAA CGGTTGACGC GCTCACCGAT GACGGACATT 1001 ATCTCGGGGG AACCATCATG CCCGGTTTCC ACCTGATGAA AGAATCGCTC 1051 GCCGTCCGAA CCGCCAACCT CAACCGGCAC GCCGGTAAGC GTTATCCTTT 1101 CCCGACCACA ACGGGCAATG CCGTCGCCAG CGGCATGATG GATGCGGTTT 1151 GCGGCTCGGT TATGATGATG CACGGGCGTT TGAAAGAAAA AACCGGGGCG 1201 GGCAAGCCTG TCGATGTCAT CATTACCGGC GGCGGCGCGG CAAAAGTTGC 1251 CGAAGCCCTG CCGCCTGCAT TTTTGGCGGA AAATACCGTG CGCGTGGCGG 1301 ACAACCTCGT CATTCACGGG CTGCTGAACC TGATTGCCGC CGAAGGCGGG 1351 GAATCGGAAC ATACTTAA This corresponds to the amino acid sequence <SEQ ID 1294; ORF 31 l.a>: a311.pep 1 MFSFGWVFDR PQYELGSLSP VAAVACRRAL SRLGLKTQIK WPNDLWGRD 51 KLGGILIETV RTGGKTVAW GIGINFVLPK EVENAASVQS LFQTASRRGN 101 ADAAVLLETL LAELDAVLLQ YARDGFAPFV AEYQAANRDH GKAVLLLRDG 151 ETVFEGTVKG VDGQGVLHLE TAEGKQTWS GEISLRSDDR PVSVPKRRDS 201 ERFLLLDGGN SRLKWAWVEN GTFATVGSAP YRDLSPLGAE WAEKVDGNVR 251 IVGCAVCGEF KKAQVQEQLA RKIEWLPSSA QALGIRNHYR HPEEHGSDRW 301 FNALGSRRFS RNACVWSCG TAVTVDALTD DGHYLGGTIM PGFHLMKESL 351 AVRTANLNRH AGKRYPFPTT TGNAVASGMM DAVCGSVMMM HGRLKEKTGA 401 GKPVDVIITG GGAAKVAEAL PPAFLAENTV RVADNLVIHG LLNLIAAEGG 451 ESEHT* m31 l/a311 81.3% identity in 455 aa overlap 10 20 30 40 50 60 m311.pep MFSFGWVFDRPQYELGSLSPVAAVACRRALSRLGLDVQIKWPNDLWGRDKLGGILIETV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I a311 MFSFGWVFDRPQYELGSLSPVAAVACRRALSRLGLKTQIKWPNDLVVGRDKLGGILIETV 10 20 30 40 50 60 70 80 90 100 110 m311 . pep RTGGKTVAWGIGINFVLPXEVENAASVQSLFQTASRRGNADAAVLLXXXXXXXX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : a311 RTGGKTVAWGIGINFVLPKEVENAASVQSLFQTASRRGNADAAVLLETLLAELDAVLLQ 70 80 90 100 110 120 m311 . pep a311 YARDGFAPFVAEYQAANRDHGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGKQTWS 130 140 150 160 170 180 120 130 140 150 160 170 m311.pep -EISLRSDXRPVSVXKRRDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAE lllllll I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a311 GEISLRSDDRPVSVPKRRDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAE 190 200 210 220 230 240 180 190 200 210 220 230 m311.pep WAEKADGNVRIVGCAVCGEFKKAQVQEQLARKIEWLPSSAQALFGIRNHYRHPEEHGSDR I I I I: I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a311 WAEKVDGNVRIVGCAVCGEFKKAQVQEQLARKIEWLPSSAQAL-GIRNHYRHPEEHGSDR 250 260 270 280 290 240 250 260 270 280 290 m311.pep WFNALGSRRFSRNACVWSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a311 WFNALGSRRFSRNACVWSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNR 300 310 320 330 340 350 300 310 320 330 340 350 m311. pep HAGKRYPFPTTTGNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a311 HAGKRYPFPTTTGNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEA 360 370 380 390 400 410 360 370 380 389 m311. pep LPPAFLAENTVRVADNLVIYGLLNMIAAEGREYEH I I I I I I I I I I I I I I I I I I I: I I I I : I I I I I I II a311 LPPAFLAENTVRVADNLVIHGLLNLIAAEGGESEHTX 420 430 440 450 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1295>: g311-l.seq 1 ATGACGGTTT TGAAGCCTTC GCATTGGCGG GTGTTGGCGG AGCTTGCCGA 51 CGGTTTGCCG CAACACGTAT CGCAATTGGC GCGTGAGGCG GACATGAAGC 101 CGCAGCAGCT CAACGGTTTT TGGCAGCAGA TGCCGGCGCA TATACGCGGG 151 CTGTTGCGCC AACACGACGG CTATTGGCGG CTGGTGCGCC CCTTGGCGGT 201 TTTCGATGCC GAAGGTTTGC GCGATCTGGG GGAAAGGTCG GGTTTTCAGA 251 CGGCATTGAA GCACGAGTGC GCGTCCAGCA ACGACGAGAT ACTGGAATTG 301 GCGCGGATTG CGCCGGACAA GGCGCACAAA ACCATATGCG TGACCCACCT 351 GCAAAGTAAG GGCAGGGGGC GGCAGGGGCG GAAGTGGTCG CACCGTTTGG 401 GCGAGTGCCT GATGTTCAGT TTCGGCTGGG CGTTTGACCG GCCGCAGTAT 451 GAGTTGGGTT CGCTGTCGCC TGTTGCGGCA CTTGCGTGCC GGCGCGCTTT 501 GGGGTGTTTG GGTTTGGAAA CGCAAATCAA GTGGCCAAAC GATTTGGTCG 551 TCGGACGCGA CAAATTGGGC GGCATTCTGA TTGAAACAGT CAGGGCGGGC 601 GGTAAAACGG TTGCCGTGGT CGGTATCGGC ATCAATTTCG TGCTGCCCAA 651 GGAAGTGGAA AACGCCGCTT CCGTGCAGTC GCTGTTTCAG ACGGCATCGC 701 GGCGGGGCAA TGCCGATGCC GCCGTATTGC TGGAAACATT GCTTGCGGAA 751 CTGGGCGCGG TGTTGGAACA ATATGCGGAA GAAGGGTTCG CGCCATTTTT 801 AAATGAGTAT GAAACGGCCA ACCGCGACCA CGGCAAGGCG GTATTGCTGT 851 TGCGCGACGG CGAAACCGTG TGCGAAGGCA CGGTTAAAGG CGTGGACGGA 901 CGAGGCGTTC TGCACTTGGA AACGGCAGaa ggCGAACAGa cggtcGtcag 951 cggcGaaaTC AGccTGCGGc CCGacaacag gtcggtttcc GTgccgaagc 1001 gGccggatTC GgaacgttTT tTGCTgttgg aaggcgggaa cagccggctc 1051 aAGTGGgcgt gGGTggAAAA Cggcacgttc gcaaccgtgg gcAGCGCgCC 1101 gtaCCGCGAT TTGTCGCCTT TGGGCGCGGA GTGGGCGGAA AAGGCGGATG 1151 GAAATGTCCG CATCGTCGGT TGCGCCGTGT GCGGAGAATC CAAAAAGGCA 1201 CAAGTGAAGG AACAGCTCGC CCGAAAAATC GAGTGGCTGC CGTCTTCCGC 1251 ACAGGCTTTG GGCATACGCA ACCACTACCG CCACCCCGAA GAACACGGTT 1301 CCGACCGTTG GTTCAACGCC TTGGGCAGCC GCCGCTTCAG CCGCAACGCC 1351 TGCGTCGTCG TCAGTTGCGG CACGGCGGTA ACGGTTGACG CGCTCACCGA 1401 TGACGGACAT TATCTCGGCG GAACCATCAT GCCCGGCTTC CACCTGATGA 1451 AAGAATCGCT CGCCGTCCGA ACCGCCAACC TCAACCGCCC CGCCGGCAAA 1501 CGTTACCCTT TCCCGACCAC AACGGGCAAC GCCGTCGCAA GCGGCATGAT 1551 GGACGCGGTT TGCGGCTCGA TAATGATGAT GCACGGCCGT TTGAAAGAAA 1601 AAAACGGCGC GGGCAAGCCT GTCGATGTCA TCATTACCGG CGGCGGCGCG 1651 GCGAAAGTCG CCGAAGCCCT GCCGCCTGCA TTTTTGGCGG AAAATACCGT 1701 GCGCGTGGCG GACAACCTCG TCATCCACGG GCTGCTGAAC CTGATTGCCG 1751 CCGAAGGCGG GGAATCGGAA CACGCTTAA This corresponds to the amino acid sequence <SEQ ID 1296; ORF 311-l.ng>: g311-l.pep 1 MTVLKPΞHWR VLAELADGLP QHVΞQLAREA DMKPQQLNGF WQQMPAHIRG 51 LLRQHDGYWR LVRPLAVFDA EGLRDLGERS GFQTALKHEC ASSNDEILEL 101 ARIAPDKAHK TICVTHLQΞK GRGRQGRKWS HRLGECLMFS FGWAFDRPQY 151 ELGSLSPVAA LACRRALGCL GLETQIKWPN DLVVGRDKLG GILIETVRAG 201 GKTVAWGIG INFVLPKEVE NAASVQSLFQ TASRRGNADA AVLLETLLAE 251 LGAVLEQYAE EGFAPFLNEY ETANRDHGKA VLLLRDGETV CEGTVKGVDG 301 RGVLHLETAE GEQTWSGEI SLRPDNRSVS VPKRPDSERF LLLEGGNΞRL 351 KWAWVENGTF ATVGSAPYRD LSPLGAEWAE KADGNVRIVG CAVCGESKKA 401 QVKEQLARKI EWLPSSAQAL GIRNHYRHPE EHGSDRWFNA LGSRRFSRNA 451 CVWSCGTAV TVDALTDDGH YLGGTIMPGF HLMKESLAVR TANLNRPAGK 501 RYPFPTTTGN AVASGMMDAV CGSIMMMHGR LKEKNGAGKP VDVIITGGGA 551 AKVAEALPPA FLAENTVRVA DNLVIHGLLN LIAAEGGESE HA* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1297>: m311-l.seq 1 ATGACGGTTT TGAAGCTTTC GCACTGGCGG GTGTTGGCGG AGCTTGCCGA 51 CGGTTTGCCG CAACACGTCT CGCAACTGGC GCGTATGGCG GATATGAAGC 101 CGCAGCAGCT CAACGGTTTT TGGCAGCAGA TGCCGGCGCA CATACGCGGG 151 CTGTTGCGCC AACACGACGG CTATTGGCGG CTGGTGCGCC CATTGGCGGT 201 TTTCGATGCC GAAGGTTTGC GCGAGCTGGG GGAAAGGTCG GGTTTTCAGA 251 CGGCATTGAA GCACGAGTGC GCGTCCAGCA ACGACGAGAT ACTGGAATTG 301 GCGCGGATTG CGCCGGACAA GGCGCACAAA ACCATATGCG TGACCCACCT 351 GCAAAGTAAG GGCAGGGGGC GGCAGGGGCG GAAGTGGTCG CACCGTTTGG 401 GCGAGTGTCT GATGTTCAGT TTTGGCTGGG TGTTTGACCG GCCGCAGTAT 451 GAGTTGGGTT CGCTGTCGCC TGTTGCGGCA GTGGCGTGTC GGCGCGCCTT 501 GTCGCGTTTA GGTTTGGATG TGCAGATTAA GTGGCCCAAT GATTTGGTTG 551 TCGGACGCGA CAAATTGGGC GGCATTCTGA TTGAAACGGT CAGGACGGGC 601 GGCAAAACGG TTGCCGTGGT CGGTATCGGC ATCAATTTTG TCCTGCCCAA 651 GGAAGTAGAA AATGCCGCTT CCGTGCAATC GCTGTTTCAG ACGGCATCGC 701 GGCGGGGCAA TGCCGATGCC GCCGTGCTGC TGGAAACGCT GTTGGTGGAA 751 CTGGACGCGG TGTTGTTGCA ATATGCGCGG GACGGATTTG CGCCTTTTGT 801 GGCGGAATAT CAGGCTGCCA ACCGCGACCA CGGCAAGGCG GTATTGCTGT 851 TGCGCGACGG CGAAACCGTG TTCGAAGGCA CGGTTAAAGG CGTGGACGGA 901 CAAGGCGTTT TGCACTTGGA AACGGCAGAG GGCAAACAGA CGGTCGTCAG 951 CGGCGAAATC AGCCTGCGGT CCGACGACAG GCCGGTTTCC GTGCCGAAGC 1001 GGCGGGATTC GGAACGTTTT CTGCTGTTGG ACGGCGGCAA CAGCCGGCTC 1051 AAGTGGGCGT GGGTGGAAAA CGGCACGTTC GCAACCGTCG GTAGCGCGCC 1101 GTACCGCGAT TTGTCGCCTT TGGGCGCGGA GTGGGCGGAA AAGGCGGATG 1151 GAAATGTCCG CATCGTCGGT TGCGCTGTGT GCGGAGAATT CAAAAAGGCA 1201 CAAGTGCAGG AACAGCTCGC CCGAAAAATC GAGTGGCTGC CGTCTTCCGC 1251 ACAGGCTTTG GGCATACGCA ACCACTACCG CCACCCCGAA GAACACGGTT 1301 CCGACCGCTG GTTCAACGCC TTGGGCAGCC GCCGCTTCAG CCGCAACGCC 1351 TGCGTCGTCG TCAGTTGCGG CACGGCGGTA ACGGTTGACG CGCTCACCGA 1401 TGACGGACAT TATCTCGGGG GAACCATCAT GCCCGGTTTC CACCTGATGA 1451 AAGAATCGCT CGCCGTCCGA ACCGCCAACC TCAACCGGCA CGCCGGTAAG 1501 CGTTATCCTT TCCCGACCAC AACGGGCAAT GCCGTCGCCA GCGGCATGAT 1551 GGATGCGGTT TGCGGCTCGG TTATGATGAT GCACGGGCGT TTGAAAGAAA 1601 AAACCGGGGC GGGCAAGCCT GTCGATGTCA TCATTACCGG CGGCGGCGCG 1651 GCAAAAGTTG CCGAAGCCCT GCCGCCTGCA TTTTTGGCGG AAAATACCGT 1701 GCGCGTGGCG GACAACCTCG TCATTTACGG GTTGTTGAAC ATGATTGCCG 1751 CCGAAGGCAG GGAATATGAA CATATTTAA This corresponds to the amino acid sequence <SEQ ID 1298; ORF 311-1>: m311-l.pep 1 MTVLKLΞHWR VLAELADGLP QHVSQLARMA DMKPQQLNGF WQQMPAHIRG 51 LLRQHDGYWR LVRPLAVFDA EGLRELGERS GFQTALKHEC ASSNDEILEL 101 ARIAPDKAHK TICVTHLQSK GRGRQGRKWS HRLGECLMFS FGWVFDRPQY 151 ELGSLSPVAA VACRRALSRL GLDVQIKWPN DLWGRDKLG GILIETVRTG 201 GKTVAWGIG INFVLPKEVE NAASVQSLFQ TASRRGNADA AVLLETLLVE 251 LDAVLLQYAR DGFAPFVAEY QAANRDHGKA VLLLRDGETV FEGTVKGVDG 301 QGVLHLETAE GKQTVVSGEI SLRSDDRPVS VPKRRDΞERF LLLDGGNSRL 351 KWAWVENGTF ATVGSAPYRD LSPLGAEWAE KADGNVRIVG CAVCGEFKKA 401 QVQEQLARKI EWLPSSAQAL GIRNHYRHPE EHGSDRWFNA LGSRRFSRNA 451 CVWSCGTAV TVDALTDDGH YLGGTIMPGF HLMKESLAVR TANLNRHAGK 501 RYPFPTTTGN AVASGMMDAV CGΞVMMMHGR LKEKTGAGKP VDVIITGGGA 551 AKVAEALPPA FLAENTVRVA DNLVIYGLLN MIAAEGREYE HI* m311-l/g311-l 93.9% identity in 591 aa overlap 10 20 30 40 50 60 m311-1.pep MTVLKLSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQMPAHIRGLLRQHDGYWR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I g311-l MTVLKPSHWRVLAELADGLPQHVSQLAREADMKPQQLNGFWQQMPAHIRGLLRQHDGYWR 10 20 30 40 50 60 70 80 90 100 110 120 m311-1. pep LVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELARIAPDKAHKTICVTHLQSK I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g311-l LVRPLAVFDAEGLRDLGERSGFQTALKHECASSNDEILELARIAPDKAHKTICVTHLQSK 70 80 90 100 110 120 130 140 150 160 170 180 m311-l.pep GRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGΞLSPVAAVACRRALSRLGLDVQIKWPN I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I : I I I I I I: I I I :: I I I I I I g311-l GRGRQGRKWSHRLGECLMFΞFGWAFDRPQYELGSLSPVAALACRRALGCLGLETQIKWPN 130 140 150 160 170 180 190 200 210 220 230 240 m311-1 . pep DLVVGRDKLGGILIETVRTGGKTVAWGIGINFVLPKEVENAASVQSLFQTAΞRRGNADA I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g311-l DLWGRDKLGGILIETVRAGGKTVAWGIGINFVLPKEVENAAΞVQSLFQTAΞRRGNADA 190 200 210 220 230 240 250 260 270 280 290 300 m311-1. pep AVLLETLLVELDAVLLQYARDGFAPFVAEYQAANRDHGKAVLLLRDGETVFEGTVKGVDG I I I I I I I h I I III I I I :: I I I I h I I : : II I I I I I I I I I I I I I I I I ll g311-1 AVLLETLLAELGAVLEQYAEEGFAPFLNEYETANRDHGKAVLLLRDGETVCEGTVKGVDG 250 260 270 280 290 300 310 320 330 340 350 360 m311-l.pep QGVLHLETAEGKQTVVSGEISLRΞDDRPVSVPKRRDSERFLLLDGGNSRLKWAWVENGTF : I I I I I I I I I I : I I I I I I I I I I I hi llllll I I I I I I I I : I I I I I I I I I I I I I I I I g311 - 1 RGVLHLETAEGEQTWSGEI SLRPDNRSVΞVPKRPDSERFLLLEGGNΞRLKWAWVENGTF 310 320 330 340 350 360 370 380 390 400 410 420 m311-l . pep ATVGSAPYRDLΞPLGAEWAEKADGNVRIVGCAVCGEFKKAQVQEQLARKIEWLPSSAQAL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I h i I I I I I I I I I I I I I I I I g311-l ATVGSAPYRDLSPLGAEWAEKADGNVRIVGCAVCGESKKAQVKEQLARKIEWLPSSAQAL 370 380 390 400 410 420 430 440 450 460 470 480 m311-1. pep GIRNHYRHPEEHGΞDRWFNALGΞRRFSRNACVWSCGTAVTVDALTDDGHYLGGTIMPGF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g311-l GIRNHYRHPEEHGSDRWFNALGSRRFSRNACWVSCGTAVTVDALTDDGHYLGGTIMPGF 430 440 450 460 470 480 490 500 510 520 530 540 m311 - 1 . pep HLMKEΞLAVRTANLNRHAGKRYPFPTTTGNAVASGMMDAVCGSVMMMHGRLKEKTGAGKP I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I M I h i I I I I g311-l HLMKESLAVRTANLNRPAGKRYPFPTTTGNAVASGMMDAVCGSIMMMHGRLKEKNGAGKP 490 500 510 520 530 540 550 560 570 580 590 m311-l .pep VDVIITGGGAAKVAEALPPAFLAENTVRVADNLVIYGLLNMIAAEGREYEHIX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I II h I I I I I I II g311-1 VDVIITGGGAAKVAEALPPAFLAENTVRVADNLVIHGLLNLIAAEGGESEHAX 550 560 570 580 590 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1299>: a311-l.seq 1 ATGACGGTTT TGAAGCCTTC GCACTGGCGG GTGTTGGCGG AGCTTGCCGA 51 CGGTTTGCCG CAACACGTCT CGCAACTGGC GCGTATGGCG GATATGAAGC 101 CGCAGCAGCT CAACGGTTTT TGGCAGCAGA TGCCGGCGCA CATACGCGGG 151 CTGTTGCGCC AACACGACGG CTATTGGCGG CTGGTGCGCC CATTGGCGGT 201 TTTCGATGCC GAAGGTTTGC GCGAGCTGGG GGAAAGGTCG GGTTTTCAGA 251 CGGCATTGAA GCACGAGTGC GCGTCCAGCA ACGACGAGAT ACTGGAATTG 301 GCGCGGATTG CGCCGGACAA GGCGCACAAA ACCATATGTG TGACCCACCT 351 GCAAAGTAAG GGCAGGGGGC GGCAGGGGCG GAAGTGGTCG CACCGTTTGG 401 GCGAGTGTCT GATGTTCAGT TTTGGCTGGG TGTTTGACCG GCCGCAGTAT 451 GAGTTGGGTT CGCTGTCGCC TGTTGCGGCA GTGGCGTGCC GGCGCGCCTT 501 GTCGCGTTTG GGTTTGAAAA CGCAAATCAA GTGGCCAAAC GATTTGGTCG 551 TCGGACGCGA CAAATTGGGC GGCATTCTGA TTGAAACGGT CAGGACGGGC 601 GGCAAAACGG TTGCCGTGGT CGGTATCGGC ATCAATTTCG TGCTGCCCAA 651 GGAAGTGGAA AACGCCGCTT CCGTGCAATC GCTGTTTCAG ACGGCATCGC 701 GGCGGGGAAA TGCCGATGCC GCCGTGTTGC TGGAAACGCT GTTGGCGGAA 751 CTTGATGCGG TGTTGTTGCA ATATGCGCGG GACGGATTTG CGCCTTTTGT 801 GGCGGAATAT CAGGCTGCCA ACCGCGACCA CGGCAAGGCG GTATTGCTGT 851 TGCGCGACGG CGAAACCGTG TTCGAAGGCA CGGTTAAAGG CGTGGACGGA 901 CAAGGCGTTC TGCACTTGGA AACGGCAGAG GGCAAACAGA CGGTCGTCAG 951 CGGCGAAATC AGCCTGCGGT CCGACGACAG GCCGGTTTCC GTGCCGAAGC 1001 GGCGGGATTC GGAACGTTTT CTGCTGTTGG ACGGCGGCAA CAGCCGGCTC 1051 AAGTGGGCGT GGGTGGAAAA CGGCACGTTC GCAACCGTCG GTAGCGCGCC 1101 GTACCGCGAT TTGTCGCCTT TGGGCGCGGA GTGGGCGGAA AAGGTGGATG 1151 GAAATGTCCG CATCGTCGGT TGCGCCGTGT GCGGAGAATT CAAAAAGGCA 1201 CAAGTGCAGG AACAGCTCGC CCGAAAAATC GAGTGGCTGC CGTCTTCCGC 1251 ACAGGCTTTG GGCATACGCA ACCACTACCG CCACCCCGAA GAACACGGTT 1301 CCGACCGCTG GTTCAACGCC TTGGGCAGCC GCCGCTTCAG CCGCAACGCC 1351 TGCGTCGTCG TCAGTTGCGG CACGGCGGTA ACGGTTGACG CGCTCACCGA 1401 TGACGGACAT TATCTCGGGG GAACCATCAT GCCCGGTTTC CACCTGATGA 1451 AAGAATCGCT CGCCGTCCGA ACCGCCAACC TCAACCGGCA CGCCGGTAAG 1501 CGTTATCCTT TCCCGACCAC AACGGGCAAT GCCGTCGCCA GCGGCATGAT 1551 GGATGCGGTT TGCGGCTCGG TTATGATGAT GCACGGGCGT TTGAAAGAAA 1601 AAACCGGGGC GGGCAAGCCT GTCGATGTCA TCATTACCGG CGGCGGCGCG 1651 GCAAAAGTTG CCGAAGCCCT GCCGCCTGCA TTTTTGGCGG AAAATACCGT 1701 GCGCGTGGCG GACAACCTCG TCATTCACGG GCTGCTGAAC CTGATTGCCG 1751 CCGAAGGCGG GGAATCGGAA CATACTTAA This corresponds to the amino acid sequence <SEQ ID 1300; ORF 31 l-l.a>: a311-l.pep 1 MTVLKPSHWR VLAELADGLP QHVSQLARMA DMKPQQLΝGF WQQMPAHIRG 51 LLRQHDGYWR LVRPLAVFDA EGLRELGERS GFQTALKHEC ASSΝDEILEL 101 ARIAPDKAHK TICVTHLQΞK GRGRQGRKWS HRLGECLMFS FGWVFDRPQY 151 ELGSLSPVAA VACRRALΞRL GLKTQIKWPΝ DLWGRDKLG GILIETVRTG 201 GKTVAWGIG IΝFVLPKEVE ΝAASVQSLFQ TASRRGΝADA AVLLETLLAE 251 LDAVLLQYAR DGFAPFVAEY QAAΝRDHGKA VLLLRDGETV FEGTVKGVDG 301 QGVLHLETAE GKQTVVSGEI SLRΞDDRPVS VPKRRDSERF LLLDGGΝSRL 351 KWAWVEΝGTF ATVGSAPYRD LSPLGAEWAE KVDGΝVRIVG CAVCGEFKKA 401 QVQEQLARKI EWLPSSAQAL GIRΝHYRHPE EHGSDRWFΝA LGΞRRFSRΝA 451 CVVVSCGTAV TVDALTDDGH YLGGTIMPGF HLMKESLAVR TAΝLΝRHAGK 501 RYPFPTTTGΝ AVAΞGMMDAV CGSVMMMHGR LKEKTGAGKP VDVIITGGGA 551 AKVAEALPPA FLAEΝTVRVA DΝLVIHGLLΝ LIAAEGGESE HT* a311-l/m311-l 98.5% identity in 591 aa overlap 10 20 30 40 50 60 a311-1.pep MTVLKPSHWRVLAELADGLPQHVΞQLARMADMKPQQLΝGFWQQMPAHIRGLLRQHDGYWR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m311-l MTVLKLSHWRVLAELADGLPQHVSQLARMADMKPQQLΝGFWQQMPAHIRGLLRQHDGYWR 10 20 30 40 50 60 70 80 90 100 110 120 a311-1. pep LVRPLAVFDAEGLRELGERSGFQTALKHECASSΝDEILELARIAPDKAHKTICVTHLQΞK II I II I II I I II I I I II I I I I I I I I I II I I I I I I I I I I I I I I I I I I I || I I M I I I I I I I m311-l LVRPLAVFDAEGLRELGERSGFQTALKHECASΞΝDEILELARIAPDKAHKTICVTHLQSK 70 80 90 100 110 120 130 140 150 160 170 180 a311-1. pep GRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAAVACRRALSRLGLKTQIKWPN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I m311- l GRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAAVACRRALSRLGLDVQIKWPN 130 140 150 160 170 180 190 200 210 220 230 240 a311- 1 . pep DLWGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKEVENAASVQSLFQTASRRGNADA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m311- l DLWGRDKLGGILIETVRTGGKTVAWGIGINFVLPKEVENAASVQSLFQTASRRGNADA 190 200 210 220 230 240 250 260 270 280 290 300 a311- 1 . pep AVLLETLLAELDAVLLQYARDGFAPFVAEYQAANRDHGKAVLLLRDGETVFEGTVKGVDG I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m311-1 AVLLETLLVELDAVLLQYARDGFAPFVAEYQAANRDHGKAVLLLRDGETVFEGTVKGVDG 250 260 270 280 290 300 310 320 330 340 350 360 a311-1. pep QGVLHLETAEGKQTWSGEISLRSDDRPVSVPKRRDSERFLLLDGGNSRLKWAWVENGTF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m311-l QGVLHLETAEGKQTVVSGEISLRSDDRPVSVPKRRDSERFLLLDGGNSRLKWAWVENGTF 310 320 330 340 350 360 370 380 390 400 410 420 a311-1. pep ATVGSAPYRDLSPLGAEWAEKVDGNVRIVGCAVCGEFKKAQVQEQLARKIEWLPSSAQAL I I I I I I I I I I I I I I I I I I I I h I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m311-l ATVGSAPYRDLSPLGAEWAEKADGNVRIVGCAVCGEFKKAQVQEQLARKIEWLPSSAQAL 370 380 390 400 410 420 430 440 450 460 470 480 a311-1. pep GIRNHYRHPEEHGSDRWFNALGSRRFSRNACVVVSCGTAVTVDALTDDGHYLGGTIMPGF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m311- l GIRNHYRHPEEHGSDRWFNALGSRRFSRNACVWSCGTAVTVDALTDDGHYLGGTIMPGF 430 440 450 460 470 480 490 500 510 520 530 540 a311-l . pep HLMKESLAVRTANLNRHAGKRYPFPTTTGNAVASGMMDAVCGSVMMMHGRLKEKTGAGKP I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m311-1 HLMKESLAVRTANLNRHAGKRYPFPTTTGNAVASGMMDAVCGΞVMMMHGRLKEKTGAGKP 490 500 510 520 530 540 550 560 570 580 590 a311-l .pep VDVIITGGGAAKVAEALPPAFLAENTVRVADNLVIHGLLNLIAAEGGEΞEHTX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I h I I I I : I I I I I I I I m311-1 VDVIITGGGAAKVAEALPPAFLAENTVRVADNLVIYGLLNMIAAEGREYEHIX 550 560 570 580 590 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1301>: g312.seq 1 atgaGtatCc aatCcGgcga AATTTtagaa accgtCAAAA TGGTTGCCGA 51 ccggaATttt gAtgtccgCA CCATTAccat cggcaTTgaT ttgcacgact 101 gcatcagcac cgacatcgac gtgttaAACC AAAACATtta caaCAaaaTc 151 accacggtcg gcaaagactT GGTGGCAacg Gcgaaacacc tTTccgcCAA 201 ATACGGCGTG CCGATTGTGA ATCAGCGCAT TTCCGTTACG CCGAttgccc 251 AaatcGCGGC GGcgaccaAa gccgaCAGTT AtgtcAGCgt ggcgcAGact 301 tTGGACAAGG CAGCCAAAGC CATCGGCGTG TCCTTTATCG GcggCTTTTC 351 CGCGCTGGTG CAAAAAGGTA TGTCGCCTTC GGATGAGGTG TTGATCCGTT 401 CCGTTCCCGA AGCGATGAAA ACTACCGATA TCGTGTGCAG CTCCATCAAT 451 ATCGGCAGCA CGCGTGCCGG TATCAATATG GATGCGGTCA AGCTGGCAGG 501 CGAAACCATC AAACGCACGG CTGAAATCAC ACCCGAAGGT TTCGGCTGCG 551 CCAAAATCGT CGTGTTCTGC AACGCGGTGG AAGACAATCC GTTTATGGCG 601 GGTGCGTTCC ACGGCTCGGG CGAAGCGGAT GCTGTGATTA ATGTCGGCGT 651 ATCCGGTCCA GGCGTGGTCA AAGCCGCGCT GGAAAATTCG GACGCGGTCA 701 GCCTGACCGA GGTCGCCGAA GTCGTGAAGA AAACCGCTTT CAAAATCACC 751 CGCGTGGGCG AACTCATCGG TCGCGAAGCC TCAAAAATGC TGAATATCCC 801 GTTCGGCATT CTCGATTTGT CGCTGGCACC GACCCCCGCC GTCGGCGACT 851 CGGTGGCGCG CATTCTTGAA GAAATGGGCT TGAGCGTCTG CGGTACGCAC 901 GGCACAACAG CAGCTTTGGC ATTGCTGAAC GATGCCGTGA AAAAGGGCGG 951 CATGATGGCT TCCAGCGCGG TCGGCGGTTT GAGCGGCGCG TTTATCCCCG 1001 TTTCCGAAGA CGAAGGTATG ATTGCCGCCG CCGAGGCAGG CGTGTTGACG 1051 CTGGACAAAC TCGAAGCCAT GACCGCCGTC TGCTCCGTTG GTTTGGACAT 1101 GATTGCCGTT CCCGGCGACA CGCCCGCGCA CACCATTTCC GGCATCATCG 1151 CCGACGAAGC CGCCATCGGC ATGATCAACA GCAAAACCAC CGCCGTGCGC 1201 ATTATTCCGG TAACGGGCAA AACCGTCGGC GACAGCGTCG AGTTCGGCGG 1251 TCTGTTGGGC TACGCGCCTG TAATGCCGGC AAAAGAAGGT TCGTGCGAAG 1301 TGTTCGTCAA CCGGGGCGGC AGGATTCCCG CACCGGTTCA ATCGATGAAA 1351 AACTGA This corresponds to the amino acid sequence <SEQ ID 1302; ORF 312.ng>: g312.pep 1 MSIQSGEILE TVKMVADRNF DVRTITIGID LHDCISTDID VLNQNIYNKI 51 TTVGKDLVAT AKHLSAKYGV PIVNQRISVT PIAQIAAATK ADSYVSVAQT 101 LDKAAKAIGV SFIGGFSALV QKGMSPSDEV LIRSVPEAMK TTDIVCSSIN 151 IGSTRAGINM DAVKLAGETI KRTAEITPEG FGCAKIWFC NAVEDNPFMA 201 GAFHGSGEAD AVINVGVSGP GWKAALENS DAVSLTEVAE WKKTAFKIT 251 RVGELIGREA SKMLNIPFGI LDLSLAPTPA VGDSVARILE EMGLSVCGTH 301 GTTAALALLN DAVKKGGMMA SSAVGGLSGA FIPVSEDEGM IAAAEAGVLT 351 LDKLEAMTAV CSVGLDMIAV PGDTPAHTIS GIIADEAAIG MINSKTTAVR 401 IIPVTGKTVG DSVEFGGLLG YAPVMPAKEG SCEVFVNRGG RIPAPVQSMK 451 N* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1303>: m312.seq 1 ATGAGTATCC AATCCGGCGA AATTTTAGAA ACCGTCAAAA TGGTTGCCGA 51 CCAGAATTTT GATGTCCGCA CCATTACCAT CGGCATTGAT TTGCACGACT 101 GCATCAGCAG CGATATCAAT GTGTTGAACC AAAATATTTA CAATAAAATT 151 ACCACAGTCG GCAAAGACTT GGTCACTACG GCAAAATATC TGTCTGCCAA 201 ATACGGCGTA CCGATTGTGA ATCAGCGCAT TTCCGTTACG CCGATTGCCC 251 AAATCGCGGC GGCCACCCAT GCTGATTCTT ACGTCAGCGT GGCGCAAACT 301 TTGGATAAAG CTGCCAAAGC CATCGGTGTG TCTTTTATCG GCGGTTTTTC 351 CGCGTTGGTG CAAAAAGGGA TGTCGCCTTC GGATGAGGTG TTAATCCGCT 401 CCATTCCCGA AGCGATGAAG ACTACCGATA TTGTGTGCwG CTCCATCAAT 451 ATCGGCAGTA CGCGTGCCGG TATCAATATG GATGCGGTCA AGCTGGCGGG 501 CGAAACcGTc AAACGCACGG CGGAAATCAC GCCCGAAGGT TTCGGCTGCG 551 CTAAAATTGT CGTGTTCTGC AACGCGGTGG AAGACAACCC GTTT TGGCG 601 GGCGCGTTTC ATGGTTCGGG CGATGCCGTT ATCAATGTCG GCGTATCCGG 651 CCCAGGTGTC GTAAAAGCCG CGTTGGAAAA TTCAGATGCA ACGACATTGA 701 CCGAAGTTGC GGAAGTAGTG AAGAAAACTG CTTTCAAAAT TACCCGCGTG 751 GGCGAACTCA TCGGCCGCGA AGCcTCAAAA ATGCTGAATA TCCCGTTTGG 801 TATTCTCOAC TTGTCGCCGA CCCCGCCCGT CGGCGACTCA GTGGCACGCA 851 TTCTTGAAGA AATGGGCTTG AGCGTCTGCG GTACGCACGG CACAACAGCA 901 GCTTTGGCAT TGCTGAACGA TGCCGTGAAA AAAGGCGGCA TGATGGCTTC 951 CAGCGCGGTC GGGGGTTTGA GTGGCGCGTT TATCCCCGTT TCCGAAGACG 1001 AAGGTATGAT yGmCgCcGCC GAAGCAGGCG TGCTGACGCT GGACAAACTC 1051 GAAGCCATGA CCGCCGTTTG TTCGGTCGGC TTGGATATGA TTGCCGTTCC 1101 CGGCGACACG CCCGCGCACA CCATTTCCGG CATCATTGCC GACGAAGCCG 1151 CCATCGGCAt GATCAACAGC AAAACCACTG CCGTGCGCAT TATTCCGGTA 1201 ACCGGTAAAA CCGTCGGCGA CAcGGTCGAG TTCGGCGGCT TGTTGGgCTA 1251 CGCGCCTGTG ATGCCGGTCA AAGAAGGTTC GTGCGAAGTA TTCGTCAACC 1301 GAGGCGGCAG AATTCCGGCT CCGGTTCAAT CGATGAAAAA CTGA This corresponds to the amino acid sequence <SEQ ID 1304; ORF 312>: m312.pep 1 MSIQSGEILE TVKMVADQΝF DVRTITIGID LHDCISSDIΝ VLΝQΝIYΝKI 51 TTVGKDLVTT AKYLSAKYGV PIVNQRISVT PIAQIAAATH ADSYVSVAQT 101 LDKAAKAIGV SFIGGFSALV QKGMSPSDEV LIRSIPEAMK TTDIVCXSIN 151 IGSTRAGINM DAVKLAGETV KRTAEITPEG FGCAKIWFC NAVEDNPFXA 201 GAFHGSGDAV INVGVSGPGV VKAALENSDA TTLTEVAE KKTAFKITRV 251 GELIGREASK MLNIPFGILD LSPTPPVGDS VARILEEMGL SVCGTHGTTA 301 ALALLNDAVK KGGMMASSAV GGLSGAFIPV SEDEGMIXAA EAGVLTLDKL 351 EAMTAVCSVG LDMIAVPGDT PAHTISGIIA DEAAIGMINS KTTAVRIIPV 401 TGKTVGDTVE FGGLLGYAPV MPVKEGSCEV FVNRGGRIPA PVQSMKN* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 312 shows 95.6% identity over a 451 aa overlap with a predicted ORF (ORF 312.ng) from N gonorrhoeae: m312/g312 10 20 30 40 50 60 m312. pep MSIQSGEILETVKMVADQΝFDVRTITIGIDLHDCISSDIΝVLΝQΝIYΝKITTVGKDLVTT MIMIMhlMMIMMMMMIhlhllMMMMMMMIhl g312 MSIQSGEILETVKMVADRΝFDVRTITIGIDLHDCISTDIDVLΝQΝIYΝKITTVGKDLVAT 10 20 30 40 50 60 70 80 90 100 110 120 m312. pep AKYLSAKYGVPIVΝQRISVTPIAQIAAATHADSYVSVAQTLDKAAKAIGVSFIGGFSALV g312 AKHLSAKYGVPIVΝQRISVTPIAQIAAATKADSYVSVAQTLDKAAKAIGVSFIGGFSALV 70 80 90 100 110 120 130 140 150 160 170 180 m312.pep QKGMSPSDEVLIRSIPEAMKTTDIVCXSIΝIGSTRAGIΝMDAVKLAGETVKRTAEITPEG I MMMMMMMMMMIhllMMMM g312 QKGMSPSDEVLIRSVPEAMKTTDIVCSSIΝIGSTRAGIΝMDAVKLAGETIKRTAEITPEG 130 140 150 160 170 180 190 200 210 220 230 m312. pep FGCAKIWFCΝAVEDΝPFXAGAFHGSG- -DAVIΝVGVSGPGWKAALEΝSDATTLTEVAE MMMMMMMMM M M I II II II II II II I II I II I II h = 111 II I g312 FGCAKIWFCΝAVEDΝPFMAGAFHGSGEADAVIΝVGVSGPGWKAALEΝSDAVSLTEVAE 190 200 210 220 230 240 240 250 260 270 280 290 m312.pep WKKTAFKITRVGELIGREASKMLΝIPFGILDLS--PTPPVGDSVARILEEMGLSVCGTH MMMMIMMMMMMMMMIMMM III MMMMMMMMMM g312 WKKTAFKITRVGELIGREASKMLNIPFGILDLSLAPTPAVGDSVARILEEMGLSVCGTH 250 260 270 280 290 300 300 310 320 330 340 350 m312. pep GTTAALALLNDAVKKGGMMASSAVGGLSGAFIPVSEDEGMIXAAEAGVLTLDKLEAMTAV MMMMMMMMIMMMIMMMMMIMMM MMMMIMIMMM g312 GTTAALALLNDAVKKGGMMASSAVGGLSGAFIPVSEDEGMIAAAEAGVLTLDKLEAMTAV 310 320 330 340 350 360 360 370 380 390 400 410 m312.pep CSVGLDMIAVPGDTPAHTISGIIADEAAIGMINSKTTAVRIIPVTGKTVGDTVEFGGLLG MMMMMMMMMMMMMMMMIMMMMMMM MINIMUM g312 CSVGLDMIAVPGDTPAHTISGIIADEAAIGMINSKTTAVRIIPVTGKTVGDSVEFGGLLG 370 380 390 400 410 420 420 430 440 m312. pep YAPVMPVKEGSCEVFVNRGGRIPAPVQSMKNX MMIhlMMMMMMMMMMMM g312 YAPVMPAKEGSCEVFVNRGGRIPAPVQSMKNX 430 440 450 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1305>: a312. seq 1 ATGAGTATCC AATCCGGCGA AATTTTAGAA ACCGTCAAAA TGGTTGCCGA 51 CCAGAATTTC GATGTCCGCA CCATTACCAT CGGCATTGAT TTGCACGACT 101 GCATCAGCAC CGACATCGAC GTGTTGAACC AAAATATTTA CAACAAAATT 151 ACCACGGTCG GCAAAGACTT GGTGGCGACA GCAAAATATC TGTCTGCCAA 201 ATACGGCGTG CCGATTGTGA ATCAGCGCAT TTCTGTCACG CCGATTGCCC 251 AAATCGCGGC GGCCACCCAT GCTGATTCTT ACGTCAGCGT GGCGCAAACT 301 TTGGATAAGG CTGCCAAAGC CATCGGCGTG TCTTTTATTG GCGGCTTTTC 351 CGCGCTGGTG CAAAAAGGTA TGTCGCCTTC TGACGAGGTG TTAATCCGTT 401 CCATTCCCGA AGCGATGAAG ACTACTGATA TCGTGTGCAG CTCCATCAAT 451 ATCGGCAGTA CGCGCGCCGG TATCAATATG GACGCGGTCA GACTGGCGGG 501 CGAAACCATC AAACGCACGG CTGAAATCAC ACTAGAAGGT TTCGGCTGCG 551 CCAAAATCGT CGTGTTCTGC AACGCGGTGG AAGACAACCC GTTTATGGCG 601 GGCGCGTTTC ACGGCTCAGG CGAAGCGGAT GCTGTGATTA ATGTCGGCGT 651 ATCCGGCCCG GGTGTCGTAA AAGCCGCGTT GGAAAATTCG GATGCAACGA 701 CATTGACCGA AGTTGCCGAA GTTGTGAAGA AAACCGCCTT CAAAATTACC 751 CGCGTGGGCG AACTCATCGG CCGCGAAGCC TCAAAAATGC TGAATATCCC 801 GTTTGGTATT CTCGACTTGT CGCTGGCACC GACCCCTGCC GTCGGCGACT 851 CGGTGGCGCG CATTCTTGAA GAAATGGGTT TGAGCGTCTG CGGTACGCAC 901 GGCACAACAG CAGCTTTGGC ATTGCTGAAC GATGCCGTGA AAAAGGGCGG 951 CATGATGGCT TCGAGCGCGG TTGGCGGTTT GAGTGGCGCG TTTATCCCCG 1001 TTTCCGAAGA CGAAGGTATG ATTGCCGCCG CCGAAGCAGG CGTGCTGACG 1051 TTGGATAAAC TCGAAGCGAT GACCGCCGTT TGTTCGGTCG GCTTGGATAT 1101 GATTGCCGTT CCCGGCGACA CACCCGCGCA CACCATTTCC GGCATCATTG 1151 CCGACGAAGC CGCCATCGGC ATGATCAACA GCAAAACCAC TGCCGTGCGC 1201 ATTATTCCGG TAACCGGTAA AACCGTCGGC GACAGCGTCG AGTTCGGCGG 1251 CCTGTTGGGC TACGCGCCTG TAATGCCGGT AAAAGAAGGC TCATGCGAAG 1301 TGTTCGTCAA CCGGGGCGGC AGGATTCCCG CACCGGTTCA ATCGATGAAA 1351 AACTGA This corresponds to the amino acid sequence <SEQ ID 1306; ORF 312. a>: a312.pep 1 MSIQSGEILE TVKMVADQNF DVRTITIGID LHDCISTDID VLNQNIYNKI 51 TTVGKDLVAT AKYLSAKYGV PIVNQRISVT PIAQIAAATH ADSYVSVAQT 101 LDKAAKAIGV SFIGGFSALV QKGMSPSDEV LIRSIPEAMK TTDIVCSSIN 151 IGSTRAGINM DAVRLAGETI KRTAEITLEG FGCAKIWFC NAVEDNPFMA 201 GAFHGSGEAD AVINVGVSGP GVVKAALENS DATTLTEVAE WKKTAFKIT 251 RVGELIGREA SKMLNIPFGI LDLSLAPTPA VGDSVARILE EMGLSVCGTH 301 GTTAALALLN DAVKKGGMMA SSAVGGLSGA FIPVSEDEGM IAAAEAGVLT 351 LDKLEAMTAV CSVGLDMIAV PGDTPAHTIS GIIADEAAIG MINSKTTAVR 401 IIPVTGKTVG DSVEFGGLLG YAPVMPVKEG SCEVFVNRGG RIPAPVQSMK 451 N* m312/a312 96.7% identity in 451 aa overlap 10 20 30 40 50 60 m312.pep MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISSDINVLNQNIYNKITTVGKDLVTT I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I: I I I I I I I I I I I I I I I I I I : I a312 MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISTDIDVLNQNIYNKITTVGKDLVAT 10 20 30 40 50 60 70 80 90 100 110 120 m312.pep AKYLSAKYGVPIVNQRISVTPIAQIAAATHADSYVSVAQTLDKAAKAIGVSFIGGFSALV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a312 AKYLSAKYGVPIVNQRISVTPIAQIAAATHADSYVSVAQTLDKAAKAIGVSFIGGFSALV 70 80 90 100 110 120 130 140 150 160 170 180 m312.pep QKGMSPSDEVLIRSIPEAMKTTDIVCXSINIGSTRAGINMDAVKLAGETVKRTAEITPEG I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I: I I I I I I I II a312 QKGMSPSDEVLIRSIPEAMKTTDIVCSSINIGSTRAGINMDAVRLAGETIKRTAEITLEG 130 140 150 160 170 180 190 200 210 220 230 m312 . pep FGCAKIWFCNAVEDNPFXAGAFHGSG — DAVINVGVSGPGWKAALENSDATTLTEVAE I I I I I I I I I I I I I I I I I I l l l l l l l l I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a312 FGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVSGPGWKAALENSDATTLTEVAE 190 200 210 220 230 240 240 250 260 270 280 290 m312 . pep WKKTAFKITRVGELIGREASKMLNIPFGILDLS — PTPPVGDSVARILEEMGLSVCGTH I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a312 WKKTAFKITRVGELIGREASKMLNIPFGILDLSLAPTPAVGDSVARILEEMGLSVCGTH 250 260 270 280 290 300 300 310 320 330 340 350 m312. pep GTTAALALLNDAVKKGGMMASSAVGGLSGAFIPVSEDEGMIXAAEAGVLTLDKLEAMTAV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a312 GTTAALALLNDAVKKGGMMASSAVGGLSGAFIPVSEDEGMIAAAEAGVLTLDKLEAMTAV 310 320 330 340 350 360 360 370 380 390 400 410 m312. pep CSVGLDMIAVPGDTPAHTISGIIADEAAIGMINSKTTAVRIIPVTGKTVGDTVEFGGLLG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I a312 CSVGLDMIAVPGDTPAHTISGIIADEAAIGMINSKTTAVRIIPVTGKTVGDSVEFGGLLG 370 380 390 400 410 420 420 430 440 m312. pep YAPVMPVKEGSCEVFVNRGGRIPAPVQSMKNX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a312 YAPVMPVKEGSCEVFVNRGGRIPAPVQSMKNX 430 440 450 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1307>: g313. seq 1 atggacgacc cgcgcaccta cggatcgggc aatcccggcg cgaccaatgt 51 tttacgcagc ggcaaaaaaa aggcggccgc gctgacgctc ttgggcgatg 101 ccgccaaagg tttggttgcc gttttgcttg cacgcgtgct tcaagaaccg 151 ctcggtttat ccgacagcgc aatcgccgcc gtcgcactcg ccgcgctggt 201 cgggcatatg tggccggtgt ttttcggatt taagggcggc aaaggcgtgg 251 caacggcatt gggcgtgctt ctggcactct ctcctgcaac tgccttggtc 301 tgcgcgttga tttggcttgt gatggcattc ggcttcaaag tatcctccct 351 tgccgcgctg gtcgccacaa ccgccgcccc ccttgccgca ctgtttttta 401 tgccgcatac ttcttggatt ttcgcaaccc tcgcaatcgc catattggtg 451 ttgctccgcc ataagagcaa catcctcaac ctgattaaag gcaaagaaag 501 caaaatcggc gaaaaacgct ga This corresponds to the amino acid sequence <SEQ ID 1308; ORF 313.ng>: g313.pep 1 MDDPRTYGSG ΝPGATΝVLRS GKKKAAALTL LGDAAKGLVA VLLARVLQEP 51 LGLSDSAIAA VALAALVGHM WPVFFGFKGG KGVATALGVL LALSPATALV 101 CA I LVMAF GFKVSSLAAL VATTAAPLAA LFFMPHTSWI FATLAIAILV 151 LLRHKSΝILΝ LIKGKESKIG EKR* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1309>: m313.seq 1 ATGGACGACC CGCGCACCTA CGGATCGGGC AATCCGGGGG CAACCAATGT 51 TTTACGCAGC GGCAAAAAAA AGGCGGCCGC GCTGACGCTC TTGGGCGATG 101 CCGCCAAAGG TTTAGTTGCC GTTTTGCTTG CACGCGTGCT TCAAGAACCG 151 CTCGGTTTAT CCGACAGCGC AATCGCGGCC GTCGCACTCG CCGCGCTGGT 201 CGGGCATATG TGGCCGGTGT TTTTCGGATT TAAAGGCGGC AAAGGCGTGG 251 CAACGGCATT GGGCGTGCTT CTGGCACTCT CTCCCGCAAC TGCCTTGGTC 301 TGCGCGTTGA TTTGGCTTGT TATGGCATTC GGCTTCAAGG TGTCCTCCCT 351 TGCCGCATTA ACCGCCACAA TCGCCGCACC GGTCGCCGCA TCCTTCTTTA 401 TGCCGCACGT CTCGTGGGTT TGGGCGACCG TCGCCATTGC TTTGCTGGTG 451 TTGTTCCGCC ACAAAAGTAA TATCGTCAAG CTGCTCGAAG GCAGAGAAAG 501 CAAAATCGGC GGCAGCCGCT GA This corresponds to the amino acid sequence <SEQ ID 1310; ORF 313>: m313.pep 1 MDDPRTYGSG ΝPGATΝVLRS GKKKAAALTL LGDAAKGLVA VLLARVLQEP 51 LGLSDSAIAA VALAALVGHM WPVFFGFKGG KGVATALGVL LALSPATALV 101 CALIWLVMAF GFKVSSLAAL TATIAAPVAA SFFMPHVSWV WATVAIALLV 151 LFRHKSΝIVK LLEGRESKIG GSR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 313 shows 90.2% identity over a 173 aa overlap with a predicted ORF (ORF 313.ng) from N gonorrhoeae: m313/g313 10 20 30 40 50 60 m313. pep MDDPRTYGSGΝPGATΝVLRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAA MMMMMMMMMMMMMMMMMMMMIMMMMIMMMMM g313 MDDPRTYGSGΝPGATΝVLRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAA 10 20 30 40 50 60 70 80 90 100 110 120 m313.pep VALAALVGHMWPVFFGFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAAL IMMMMMMMMMMMMMMMMMMMMMMMIMMMMMM g313 VALAALVGHMWPVFFGFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAAL 70 80 90 100 110 120 130 140 150 160 170 m313.pep TATIAAPVAASFFMPHVSWVWATVAIALLVLFRHKSΝIVKLLEGRESKIGGSRX Ml llhll IIMMIhM|:MIMIhMIM|::|::|:|MM Ml g313 VATTAAPLAALFFMPHTSWIFATLAIAILVLLRHKSΝILΝLIKGKESKIGEKRX 130 140 150 160 170 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1311>: a313. seq 1 ATGGACGACC CGCGCACCTA CGGATCGGGC AATCCGGGGG CAACCAATGT 51 TTTACGCAGC GGCAAAAAAA AGGCGGCCGC GCTGACGCTC TTGGGCGATG 101 CCGCCAAAGG TTTGGTTGCC GTTTTGCTTG CACGCGTGCT TCAAGAACCG 151 CTCGGTTTAT CCGACAGCGC AATCGCGGCC GTCGCACTCG CCGCGCTGGT 201 CGGGCATATG TGGCCGGTGT TTTTCGGATT TAAAGGCGGC AAAGGCGTGG 251 CAACGGCATT GGGCGTGCTT CTGGCACTCT CTCCCACAAC TGCCTTGGTC 301 TGCGCGTTGA TTTGGCTTGT GATGGCATTC GGCTTCAAGG TGTCCTCCCT 351 TGCCGCATTA ACCGCCACAA TCGCCGCCCC CCTTGCCGCA CTGTTTTTTA 401 TGCCGCATAC TTCTTGGATT TTCGCAACCC TCGCAATCGC CATATTGGTG 451 TTGCTCCGCC ATAAGAGCAA CATCCTCAAC CTGATTAAAG GCAAAGAAAG 501 CAAAATCGGC GAAAAACGCT GA This corresponds to the amino acid sequence <SEQ ID 1312; ORF 313. a>: a313.pep 1 MDDPRTYGSG ΝPGATΝVLRS GKKKAAALTL LGDAAKGLVA VLLARVLQEP 51 LGLSDSAIAA VALAALVGHM WPVFFGFKGG KGVATALGVL LALSPTTALV 101 CALIWLVMAF GFKVSSLAAL TATIAAPLAA LFFMPHTSWI FATLAIAILV 151 LLRHKSΝILΝ LIKGKESKIG EKR* m313/a313 90.8% identity in 173 aa overlap 10 20 30 40 50 60 m313.pep MDDPRTYGSGΝPGATΝVLRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a313 MDDPRTYGSGΝPGATΝVLRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAA 10 20 30 40 50 60 70 80 90 100 110 120 m313. pep VALAALVGHMWPVFFGFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAAL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I a313 VALAALVGHMWPVFFGFKGGKGVATALGVLLALSPTTALVCALIWLVMAFGFKVSSLAAL 70 80 90 100 110 120 130 140 150 160 170 m313.pep TATIAAPVAASFFMPHVSWVWATVAIALLVLFRHKSΝIVKLLEGRESKIGGSRX I I I I I I I : I I I I I I I : II :: I I : I I I: I I I : I I I I I I :: I :: I: I I I I I : I I a313 TATIAAPLAALFFMPHTSWIFATLAIAILVLLRHKSΝILΝLIKGKESKIGEKRX 130 140 150 160 170 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1313>: g401. seq 1 atgaaattac aacaattggc tgaagaaaaa atcggcgttc tgattgtgtt 51 cacgctgctt gtagtcagtg tcggtctgtt gattgaagtt gtgcccttgg 101 cctttaccaa ggcggcaaca cagccggcgc cgggcgtgaa gccttacaat 151 gccctgcagg ttgccggacg cgatatttac atccgtgagg gctgttacaa 201 ctgccactct caaatgattc gtccgttccg tgcggaaacc gagcgttacg 251 gtcattactc tgttgccgga gagtcggttt acgaccatcc gttccaatgg 301 ggttccaaac gtaccggtcc tgatttggca cgtgtgggcg gccgctattc 351 cgacgaatgg caccgcatcc acctgctgaa tccccgtgat gtcgtgcctg 401 agtccaatat gccggcattc ccgtggcttg cacgcaataa agtcgatgtc 451 gatgcaaccg ttgccaacat gaaggctttg cgtaaagtag gtactcctta 501 cagtgatgag gaaattgcga aagcgcctga ggctttggca aacaaatccg 551 agctggatgc tgtagtcgcc tatctgcaag gattgggtct ggctttgaaa 601 aacgtaaggt aa This corresponds to the amino acid sequence <SEQ ID 1314; ORF 401.ng>: g40l.ρep 1 MKLQQLAEEK IGVLIVFTLL WSVGLLIEV VPLAFTKAAT QPAPGVKPYΝ 51 ALQVAGRDIY IREGCYΝCHS QMIRPFRAET ERYGHYSVAG ESVYDHPFQW 101 GSKRTGPDLA RVGGRYSDEW HRIHLLΝPRD WPESΝMPAF PWLARΝKVDV 151 DATVAΝMKAL RKVGTPYSDE EIAKAPEALA ΝKSELDAWA YLQGLGLALK 201 ΝVR* The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 1315>: m401. seq 1 ATGAAATTAC AaCAATTGGC TGAAGAAAAA ATCGGCGTTC TGATTGTGTT 51 CACGCTGCTT GTAGTCAGTG TCGGTCTGTT GATTGAAGTT GTGCCCTTGG 101 CCTTTACCAA GGCGGCAACA CAGCCGGCGC CGGGCGTGAA GCCTTACAAT 151 GCCCTGCAGG TTGCCGGACG CGATATTTAC ATCCGTGAGG GCTGTTACAA 201 CTGCCACTCG CAAATGATTC GTCCGTTCCG TGCGGAAACC GAGCGTTACG 251 GTCATTACTC TGTTGCCGGA GAGTCGGTTT ACGACCATCC GTTCCAATGG 301 GGTTCCAAAC GTACCGGTCC TGATTTGGCA CGTGTGGGCG GTCGCTATTC 351 CGACGAATGG CACCGTATCC ACCTGCTGAA TCCCCGTGAT GTCGTGCCTG 401 AGTCCAATAT GCCGGCATTC CCGTGGCTTG CACGCAATAA AGTCGATGTC 451 GATGCAACCG TTGCCAACAT GAAGGCTTTG CGTAAAGTAG GTACTCCTTA 501 CAGTGATGAG GAAATTGCGA AAGCACCTGA GGCTTTGGCA AACAAATCCG 551 AGCTGGATGC TGTAGTCGCC TATCTGCAAG GATTGGGTCT GGCTTTGAAA 601 AACGTAAGGT AA This corresponds to the amino acid sequence <SEQ ID 1316; ORF 401>: m401. ep 1 MKLQQLAEEK IGVLIVFTLL WSVGLLIEV VPLAFTKAAT QPAPGVKPYΝ 51 ALQVAGRDIY IREGCYΝCHS QMIRPFRAET ERYGHYSVAG ESVYDHPFQW 101 GSKRTGPDLA RVGGRYSDEW HRIHLLΝPRD WPESΝMPAF PWLARΝKVDV 151 DATVAΝMKAL RKVGTPYSDE EIAKAPEALA ΝKSELDAWA YLQGLGLALK 201 ΝVR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 401 shows 100.0% identity over a 203 aa overlap with a predicted ORF (ORF 401.ng) from N gonorrhoeae: m401/g401 10 20 30 40 50 60 m401. pep MKLQQLAEEKIGVLIVFTLLWSVGLLIEWPLAFTKAATQPAPGVKPYΝALQVAGRDIY M M M M M M M M I M M M M M M M M M M M M I M M I M M I M M M M g401 MKLQQLAEEKIGVLIVFTLLWSVGLLIEWPLAFTKAATQPAPGVKPYΝALQVAGRDIY 10 20 30 40 50 60 70 80 90 100 110 120 m401.pep IREGCYΝCHS Q MIRPFRAETERYGHYSVAGESVYDHPFQWGSKRTGPDLARVGGRYSDEW g401 IREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGSKRTGPDLARVGGRYSDEW 70 80 90 100 110 120 130 140 150 160 170 180 m401 . pep HRIHLLNPRDWPESNMPAFPWLARNKVDVDATVANMKALRKVGTPYSDEEIAKAPEALA MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g401 HRIHLLNPRDWPESNMPAFPWLARNKVDVDATVANMKALRKVGTPYSDEEIAKAPEALA 130 140 150 160 170 180 190 200 m401. pep NKSELDAWAYLQGLGLALKNVRX M M M M M I M M M I M M M g401 NKSELDAWAYLQGLGLALKNVRX 190 200 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1317>: a401. seq 1 ATGAAATTAC AACAATTGGC TGAAGAAAAA ATCGGCGTTC TGATTGTGTT 51 CACGCTGCTT GTAGTCAGTG TCGGTCTGTT GATTGAAGTT GTGCCCTTGG 101 CCTTTACCAA GGCGGCAACA CAGCCGGCGT CGGGCGTGAA GCCTTACAAT 151 GCCCTGCAGG TTGCCGGACG CGATATTTAC ATCCGTGAGG GCTGTTACAA 201 CTGCCACTCG CAAATGATTC GTCCGTTCCG TGCGGAAACC GAGCGTTACG 251 GTCATTACTC TGTTGCCGGA GAGTCGGTTT ACGACCATCC GTTCCAATGG 301 GGTTCCAAAC GTACCGGTCC TGATTTGGCA CGTGTGGGCG GTCGCTATTC 351 CGACGAATGG CACCGTATCC ACCTGCTGAA TCCCCGTGAT GTCGTGCCTG 401 AGTCCAATAT GCCGGCATTC CCGTGGCTTG CACGCAATAA AGTCGATGTC 451 GATGCAACCG TTGCCAACAT GAAGGCTTTG CGTAAAGTAG GTACTCCTTA 501 CAGTGATGAG GAAATTGCGA AAGCGCCTGA GGCTTTGGCA AACAAATCCG 551 AGCTGGATGC TGTAGTCGCC TATCTGCAAG GATTGGGTCT GGCTTTGAAA 601 AACGTAAGGT AA This corresponds to the amino acid sequence <SEQ ID 1318; ORF 401. a>: a401.pep 1 MKLQQLAEEK IGVLIVFTLL WSVGLLIEV VPLAFTKAAT QPASGVKPYΝ 51 ALQVAGRDIY IREGCYΝCHS QMIRPFRAET ERYGHYSVAG ESVYDHPFQW 101 GSKRTGPDLA RVGGRYSDEW HRIHLLΝPRD WPESΝMPAF PWLARΝKVDV 151 DATVAΝMKAL RKVGTPYSDE EIAKAPEALA ΝKSELDAWA YLQGLGLALK 201 ΝVR* m401/a401 99.5% identity in 203 aa overlap 10 20 30 40 50 60 m401.pep MKLQQLAEEKIGVLIVFTLLWSVGLLIEVVPLAFTKAATQPAPGVKPYΝALQVAGRDIY I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a 01 MKLQQLAEEKIGVLIVFTLLWSVGLLIEWPLAFTKAATQPASGVKPYΝALQVAGRDIY 10 20 30 40 50 60 70 80 90 100 110 120 m401.pep IREGCYΝCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGSKRTGPDLARVGGRYSDEW I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a401 IREGCYΝCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGSKRTGPDLARVGGRYSDEW 70 80 90 100 110 120 130 140 150 160 170 180 m401.pep HRIHLLΝPRDVVPESΝMPAFPWLARΝKVDVDATVAΝMKALRKVGTPYSDEEIAKAPEALA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a401 HRIHLLΝPRDVVPESΝMPAFPWLARΝKVDVDATVAΝMKALRKVGTPYSDEEIAKAPEALA 130 140 150 160 170 180 190 200 m401. pep NKSELDAWAYLQGLGLALKNVRX I I I I I I I I I I I I I I I I I I I I I I I I a401 NKSELDAWAYLQGLGLALKNVRX 190 200 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1319>: g402. seq 1 ATGGATATGG TGAACACTAA Accgaatact agtgtgatta atatgctttc 51 tttccttacc ggatTATTGA GCTTGGGTat agaagtCtTg tGGGTAAGGA 101 TGttttcgTT CGCagcAcag tccgtgcctc aggCATTTTC atttattctt 151 gcctGttttc tgACCGgtat cgccgtcggc gCgTATTTTG GCAAACGGAT 201 TTGCCGCAGC CGCTTTGTTG ATATTCCctT TATCGGGCAG TgcttcttgT 251 GGGCGGGTAT TgccgaTttt ttgatTTTGG GTGCTGCGTG GTTGTTGACG 301 GGTTTTTccg gtttcGTCCA CCACGCCGGT AtttTCATTA CCCTgtctgc 351 CGtcGTCAGG GGGTTGATTT TCCCACTTGT ACACCATgtg GGTACGGATG 401 GCAACAAATC CGGACGACAG GTTTCCAATG TTTATTTCGC CAACGTTGCC 451 GGCAGTGCAT TGGGTCCGGT CCTTATCGGC TTTGTGATAC TTGATttgtt 501 gTCCACCCAA CAGATTtacc tgctcatCTG TTTGATTTCT GCTGCtgtcc 551 cTTTGTTTTg tacaCTGtTC CAAAAAAGTC TCCGACTGAA TGCAGTGTCG 601 GTAGCAGTTT CCCTAATGTT CGGCATCCTC ATGTTCCTAC TGCCGGATTC 651 TGTCTTTCAA AATATTGCTG GCCGTCCGGA TAGGTTGATT GAAAACAAAC 701 ACGGCATTGT TGCGGTTTAC CATAGAGATG GTGATAAGGT TGTTTATGGG 751 GCGAATGTAT ACGACGGCGC ATACAATACC GATATATTCA ATAGTGTCAA 801 CGGCATCGAA CGTGCCTATC TGCTACCCTC CCTGAAGTCC GGCATACGCC 851 GCATTTTCGT CGTTGGATTG AGTACAGGTT CGTGGGCGCG CGTCTTGTCT 901 GCCATTCCGG AAATGCAGTC GATGATCGTT GCGGAAATCA ATCCGGCATA 951 CCGTAGCCTT ATCGCGGAcg agccgcAAAT CGCACCGCTT TTGCAGGACA 1001 AACGTGTTGA AATTGTATTG GATGACGGTA GGAAATGGCT GCGTCGCCAT 1051 CCTGATGAAA AATTCGACCT GATTTTGATG AATTCGACTT GGTACTGGCG 1101 TGCCTATTCC ACTAACCTGT TGAGTGCGGA ATTTTTAAAA CAGGTGCAAA 1151 GCCACCTTAC CCCGGATGGT ATTGTAATGT TTAATACCAC GCACAGCCCG 1201 CATgctTTTG CTACCGCCGT ACACAGTATT CCCTATGCAT ACCGCTACGG 1251 GCATATGGTA GTCGGCTCGG CAACCCCGGT AGTTTTCcct AATAAAGAAC 1301 TGCTCaagca aCGCCTTTcc cgGTTGATTT GGCCGGAAAG CGGCAGgcac 1351 gtATTTGACA GCAGCACCGT GGATGCTGCA GCACAAAAGG TTGtctCtCG 1401 TATGCTGATT CGGATGACGG AAcctTCGGC TGGGGCGGAA GTCATTACTG 1451 ACGATAATAT GATTGTAGAA TACAAATACG GCAGAGGGAT TTAA This corresponds to the amino acid sequence <SEQ ID 1320; ORF 402.ng>: g402.pep 1 MDMVΝTKPΝT SVIΝMLSFLT GLLSLGIEVL WVRMFSFAAQ SVPQAFSFIL 51 ACFLTGIAVG AYFGKRICRS RFVDIPFIGQ CFLWAGIADF LILGAAWLLT 101 GFSGFVHHAG IFITLSA R GLIFPLVHHV GTDGΝKSGRQ VSΝVYFAΝVA 151 GSALGPVLIG FVILDLLSTQ QIYLLICLIS AAVPLFCTLF QKSLRLΝAVS 201 VAVSLMFGIL MFLLPDSVFQ ΝIAGRPDRLI EΝKHGIVAVY HRDGDKWYG 251 AΝVYDGAYΝT DIFΝSVΝGIE RAYLLPSLKS GIRRIFWGL STGSWARVLS 301 AIPEMQSMIV AEIΝPAYRSL IADEPQIAPL LQDKRVEIVL DDGRKWLRRH 351 PDEKFDLILM ΝSTWYWRAYS TΝLLSAEFLK QVQSHLTPDG IVMFΝTTHSP 401 HAFATAVHSI PYAYRYGHMV VGSATPWFP ΝKELLKQRLS RLIWPESGRH 451 VFDSSTVDAA AQKWSRMLI RMTEPSAGAE VITDDΝMIVE YKYGRGI* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1321>: m402. seq 1 ATGGATATAG TGAACACTAA ACCGAATACT AGTTTGATTT ATATGCnTTC 51 TTTCCTTAGC GGCTTATTGA GCTTGGGTAT AGAAGTCTTG TGGGTGAGGA 101 TGTTTTCGTT CGCAGCACAG TCCGTGCCTC AGGCATTTTC ATTTACCCTT 151 GCCTGTTTTC TGACCGGTAT CGCCGTCGGC GCGTATTTTG GCAAACGGAT 201 TTGCCGCAGC CGCTTTGTTG ATATTCCCTT TATCGGGCAG TGCTTCTTGT 251 GGGCGGGTAT TGCCGACTTT TTGATTTTGG GTGCTGCGTG GTTGTTGACG 301 GGTTTTTCCG GCTTCGTCCA CCACGCCGGT ATCTTCATTA CCCTGTCTGC 351 CGTCGTCAsA sGGTTGATTT TCCCGCTCGT ACACCATGTG GGTACGGATG 401 GCAACAAATC CGGACGACAG GTTTCCAATG TTTATTTCGC CAmCGTTGCC 451 GGCAGTGCAT TGGGTCCGGT CCTTATCGGC TTTGTGATAC TTGATTTCTT 501 GTCCACCCAA CAGATTTACC TGCTCATCTG T TGATTTCT GCTGCTGTCC 551 CTTTGTTTTG TACACTGTTC CAAAAAAGTC TCCGACTGAA TGCAGTGTCG 601 GTAGCAGTTT CCCTAATGTT CGGCATCCTC ATGTTCyTAC TGCCGGATTC 651 TGTCTTTCAA AATATTGCTG ACCGTCCGGA TAgGCTGATT GAAAACAAAC 701 ACGGCATTGT TGCGGTTTAC CATAGAGATG GTGATAAGGT TGTTTATGGG 751 GCGAATGTAT ACGACGGCGC ATACAATACC GATGTATTCA ATAGTGTCAA 801 CGGCATCGAA CGTGCCTATC TGCTACCCTC CCTGAAGTCT GGCATACGCC 851 GCATTTTCGT CGTTGGACTG AGTACAGGTT CGTGGGCGCG CGTCTTGTCT 901 GCCATTCCGG AAATGCAGTC GATGATCGTT GCGGAAATCA ATCCGGCATA 951 CCGTAGCCTT ATCGCGGACG AGCCGCAAAT CGCCCCGCTT TTGCAGGACA 1001 AACGTGTTGA AATTGTATTG GATGACGGTA GGAAATGGCT GCGTCGCCAT 1051 CCTGATGAAA AATTCGACCT GATTTTGATG AATACGACTT GGTACTGGCG 1101 TGCCTATTCC ACCAACCTGT TGAGTGCGGA ATTTTTAAAA CAGGTGCAAA 1151 GCCACCTTAC CCCGGATGGT ATTGTAATGT TTAATACCAC GCACAGCCCG 1201 CATGCTTTTG CTACCGCCGT ACACAGTATT CCCTATGCAT ACCGCTATGG 1251 GCATATGGTA GTCGGCTCGG CAACCCCGGT AGTTTTCCCT AATAAAGAAC 1301 TGCTCAAGCA ACGTCTCTCC CGGTTGATTT GGCCGGAAAG CGGCAGGCAC 1351 GTATTTGACA GCAGCACCGT GGATGCTGCA GCACAAAAGG TTGTCTCTCG 1401 TATGCTGATT CAGATGACGG aAcCTTCGGC TGGGGCGGAA GTTATTACCG 1451 ACGATAATAT GATTGTAGAA TACAAATACG GCAGAGGGAT TTAA This corresponds to the amino acid sequence <SEQ ID 1322; ORF 402>: m402.pep 1 MDIVNTKPNT SLIYMXSFLS GLLSLGIEVL WVRMFSFAAQ SVPQAFSFTL 51 ACFLTGIAVG AYFGKRICRS RFVDIPFIGQ CFLWAGIADF LILGAAWLLT 101 GFSGFVHHAG IFITLSAWX XLIFPLVHHV GTDGNKSGRQ VSNVYFAXVA 151 GSALGPVLIG FVILDFLSTQ QIYLLICXIS AAVPLFCTLF QKSLRLNAVS 201 VAVSLMFGIL MFLLPDSVFQ NIADRPDRLI ENKHGIVAVY HRDGDKWYG 251 ANVYDGAYNT DVFNSVNGIE RAYLLPSLKS GIRRIFWGL STGSWARVLS 301 AIPEMQSMIV AEINPAYRSL IADEPQIAPL LQDKRVEIVL DDGRKWLRRH 351 PDEKFDLILM NTTWYWRAYS TNLLSAEFLK QVQSHLTPDG IVMFNTTHSP 401 HAFATAVHSI PYAYRYGHMV VGSATPWFP NKELLKQRLS RLIWPESGRH 451 VFDSSTVDAA AQKWSRMLI QMTEPSAGAE VITDDNMIVE YKYGRGI* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 402 shows 97.0% identity over a 497 aa overlap with a predicted ORF (ORF 402.ng) from N gonorrhoeae: m402/g402 10 20 30 40 50 60 m402.pep MDIVΝTKPΝTSLIYMXSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIAVG I h l l M M M M I g402 MDMVΝTKPΝTSVIΝMLSFLTGLLSLGIEVLWVRMFSFAAQSVPQAFSFILACFLTGIAVG 10 20 30 40 50 60 70 80 90 100 110 120 m402.pep AYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLSAWX M M M M M M M M I M M M M M M M M M I M I M M M M I M M I M M M g402 AYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLSAWR 70 80 90 100 110 120 130 140 150 160 170 180 m402.pep XLIFPLVHHVGTDGΝKSGRQVSΝVYFAXVAGSALGPVLIGFVILDFLSTQQIYLLICXIS M M M M M M M M M M M M M I I I I I I I I I I 1 1 I I 1 1 h I I I I I I I I I 1 1 I I g402 GLIFPLVHHVGTDGΝKSGRQVSΝVYFAΝVAGSALGPVLIGFVILDLLSTQQIYLLICLIS 130 140 150 160 170 180 190 200 210 220 230 240 m402. pep AAVPLFCTLFQKSLRLΝAVSVAVSLMFGILMFLLPDSVFQΝIADRPDRLIEΝKHGIVAVY 1 1 M I j i M M 1 1 1 i 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 I I 1 1 ! 1 1 1 ! I I I 1 1 1 1 1 1 1 1 I 1 1 1 1 1 1 1 g402 AAVPLFCTLFQKSLRLΝAVSVAVSLMFGILMFLLPDSVFQΝIAGRPDRLIEΝKHGIVAVY 190 200 210 220 230 240 250 260 270 280 290 300 m402 . pep HRDGDKWYGAΝVYDGAYΝTDVFΝSVΝGIERAYLLPSLKSGIRRIFWGLSTGSWARVLS g402 HRDGDKWYGANVYDGAYNTDIFNSVNGIERAYLLPSLKSGIRRIFWGLSTGSWARVLS 250 260 270 280 290 300 310 320 330 340 350 360 m402 . pep AIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLRRHPDEKFDLILM 1 1 1 1 1 1 1 i 1 1 1 ! 1 1 1 1 1 1 1 1 1 1 1 1 1 I I 1 1 I I 1 1 1 1 1 1 1 1 1 1 1 1 1 1 I I 1 1 1 1 1 1 1 1 1 1 1 1 1 g402 AIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLRRHPDEKFDLILM 310 320 330 340 350 360 370 380 390 400 410 420 m402.pep NTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATAVHSIPYAYRYGHMV h M M M M M M I M M M M M M M M M M M M M M M M M M I M M M M g402 NSTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATAVHSIPYAYRYGHMV 370 380 390 400 410 420 430 440 450 460 470 480 m402 . pep VGSATPWFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKWSRMLIQMTEPSAGAE MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g402 VGSATPWFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKWSRMLIRMTEPSAGAE 430 440 450 460 470 480 490 m402.pep VITDDNMIVEYKYGRGIX M M M M I M M M M g402 VITDDNMIVEYKYGRGI 490 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1323>: a402.seq 1 ATGGATATAG TGAACACTAA ACCGAATACT AGTTTGATTT ATATGCTTTC 51 TTTCCTTAGC GGCTTATTGA GCTTGGGTAT AGAAGTCTTG TGGGTAAGGA 101 TGTTTTCGTT CGCAGCACAG TCCGTGCCTC AGGCATTTTC ATTTACTCTT 151 GCCTGTTTTC TGACCGGTAT CGCCGTCGGC GCGTATTTTG GCAAACGGAT 201 TTGCCGCAGC CGCTTTGTTG ATATTCCCTT TATCGGGCAG TGCTTCTTGT 251 GGGCGGGTAT TGCCGACTTT TTGATTTTGG GTGCTGCGTG GTTGTTGACG 301 GGTTTTTCCG GCTTCGTCCA CCACGCCGGT ATCTTCATTA CCCTGTCTGC 351 CGTCGTCAGA GGGTTGATTT TCCCGCTCGT ACACCATGTG GGTACGGATG 401 GCAACAAATC CGGACGACAG GTTTCCAATG TTTATTTCGC CAACGTTGCC 451 GGCAGTGCAT TGGGTCCGGT CCTTATCGGC TTTGTGATAC TTGATTTCTT 501 GTCCACCCAA CAGATTTACC TGCTCATCTG TTTGATTTCT GCTGCTGTCC 551 CTTTGTTTTG TACACTGTTC CAAAAAAGTC TCCGACTGAA TGCAGTGTCG 601 GTAGCAGTTT CCCTAATGTT CGGCATCCTC ATGTTCCTAC TGCCGGATTC 651 TGTCTTTCAA AATATTGCTG ACCGTCCGGA TAGGCTGATT GAAAACAAAC 701 ACGGCATTGT TGCGGTTTAC CATAGAGATG GTGATAAGGT TGTTTATGGG 751 GCGAATGTAT ACGACGGCGC ATACAATACC GATGTATTCA ATAGTGTCAA 801 CGGCATCGAA CGTGCCTATC TGCTACCCTC CCTGAAGTCT GGCATACGCC 851 GCATTTTCGT CGTTGGATTG AGTACAGGTT CGTGGGCGCG CGTCTTGTCT 901 GCCATTCCGG AAATGCAGTC GATGATCGTT GCGGAAATCA ATCCGGCATA 951 CCGTAGCCTT ATCGCGGACG AGCCGCAAAT CGCCCCGCTT TTGCAGGACA 1001 AACGTGTTGA AATTGTATTG GATGACGGTA GGAAATGGCT GCGTCGCCAT 1051 CCTGATGAAA AATTCGACCT GATTTTGATG AATACGACTT GGTACTGGCG 1101 TGCCTATTCC ACCAACCTGT TGAGTGCGGA ATTTTTAAAA CAGGTGCAAA 1151 GCCACCTTAC CCCGGATGGT ATTGTAATGT TTAATACCAC GCACAGCCCG 1201 CATGCTTTTG CTACCGCCGT ACACAGTATT CCCTATGCAT ACCGCTATGG 1251 GCATATGGTA GTCGGCTCGG CAACCCCGGT AGTTTTCCCT AATAAAGAAC 1301 TGCTCAAGCA ACGTCTCTCC CGGTTGATTT GGCCGGAAAG CGGCAGGCAC 1351 GTATTTGACA GCAGCACCGT GGATGCTGCA GCACAAAAGG TTGTCTCTCG 1401 TATGCTGATT CAGATGACGG AACCTTCGGC TGGTGCGGAA GTCATTACCG 1451 ACGATAATAT GATTGTAGAA TACAAATACG GCAGAGGGAT TTAA This corresponds to the amino acid sequence <SEQ ID 1324; ORF 402. a>: a402.pep 1 MDIVΝTKPΝT SLIYMLSFLS GLLSLGIEVL WVRMFSFAAQ SVPQAFSFTL 51 ACFLTGIAVG AYFGKRICRS RFVDIPFIGQ CFLWAGIADF LILGAAWLLT 101 GFSGFVHHAG IFITLSAWR GLIFPLVHHV GTDGNKSGRQ VSNVYFANVA 151 GSALGPVLIG FVILDFLSTQ QIYLLICLIS AAVPLFCTLF QKSLRLNAVS 201 VAVSLMFGIL MFLLPDSVFQ NIADRPDRLI ENKHGIVAVY HRDGDKWYG 251 ANVYDGAYNT DVFNSVNGIE RAYLLPSLKS GIRRIFWGL STGSWARVLS 301 AIPEMQSMIV AEINPAYRSL IADEPQIAPL LQDKRVEIVL DDGRKWLRRH 351 PDEKFDLILM NTTWYWRAYS TNLLSAEFLK QVQSHLTPDG IVMFNTTHSP 401 HAFATAVHSI PYAYRYGHMV VGSATPWFP NKELLKQRLS RLIWPESGRH 451 VFDSSTVDAA AQKWSRMLI QMTEPSAGAE VITDDNMIVE YKYGRGI* /a402 99.0% identity in 497 aa overlap 10 20 30 40 50 60 m402.pep MDIVNTKPNTSLIYMXSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIAVG lllllllllllllll I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a402 MDIVNTKPNTSLIYMLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIAVG 10 20 30 40 50 60 70 80 90 100 110 120 m402.pep AYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLSAWX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a402 AYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLSAWR 70 80 90 100 110 120 130 140 150 160 170 180 m402.pep XLIFPLVHHVGTDGNKSGRQVSNVYFAXVAGSALGPVLIGFVILDFLSTQQIYLLICXIS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II a402 GLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYLLICLIS 130 140 150 160 170 180 190 200 210 220 230 240 m402.pep AAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIENKHGIVAVY I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I a402 AAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIENKHGIVAVY 190 200 210 220 230 240 250 260 270 280 290 300 m402 . pep HRDGDKWYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFWGLSTGSWARVLS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a402 HRDGDKWYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFWGLSTGSWARVLS 250 260 270 280 290 300 310 320 330 340 350 360 m402 . pep AIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLRRHPDEKFDLILM I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a402 AIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLRRHPDEKFDLILM 310 320 330 340 350 360 370 380 390 400 410 420 m402. pep NTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATAVHSIPYAYRYGHMV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I a402 NTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATAVHSIPYAYRYGHMV 370 380 390 400 410 420 430 440 450 4 60 470 480 m402 . pep VGSATPWFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKWSRMLIQMTEPSAGAE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a402 VGSATPWFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKWSRMLIQMTEPSAGAE 430 440 450 460 470 480 490 m402.pep VITDDNMIVEYKYGRGIX I I I I I I I I I I I I I I I I I I a402 VITDDNMIVEYKYGRGIX 490 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1325>: g406.seq 1 ATGCGGGCAC GGCTGCTGAT ACCTATTCTT TTTTCAGTTT TTATTTTATC 51 CGCCTGCGGG ACACTGACAG GTATTCCATC GCATGGCGGA GGCAAACGCT 101 TCGCGGTCGA ACAAGAACTT GTGGCCGCTT CTGCCAGAGC TGCCGTTAAA 151 GACATGGATT TACAGGCATT ACACGGACGA AAAGTTGCAT TGTACATTGC 201 AACTATGGGC GACCAAGGTT CAGGCAGTTT GACAGGGGGT CGCTACTCCA 251 TTGATGCACT GATTCGCGGC GAATACATAA ACAGCCCTGC CGTCCGCACC 301 GATTACACCT ATCCGCGTTA CGAAACCACC GCTGAAACAA CATCAGGCGG 351 TTTGACGGGT TTAACCACTT CTTTATCTAC ACTTAATGCC CCTGCACTCT 401 CGCGCACCCA ATCAGACGGT AGCGGAAGTA GGAGCAGTCT GGGCTTAAAT 451 ATTGGCGGGA TGGGGGATTA TCGAAATGAA ACCTTGACGA CCAACCCGCG 501 CGACACTGCC TTTCTTTCCC ACTTGGTGCA GACCGTATTT TTCCTGCGCG 551 GCATAGACGT TGTTTCTCCT GCCAATGCCG ATACAGATGT GTTTATTAAC 601 ATCGACGTAT TCGGAACGAT ACGCAACAGA ACCGAAATGC ACCTATACAA 651 TGCCGAAACA CTGAAAGCCC AAACAAAACT GGAATATTTC GCAGTAGACA 701 GAACCAATAA AAAATTGCTC ATCAAACCCA AAACCAATGC GTTTGAAGCT 751 GCCTATAAAG AAAATTACGC ATTGTGGATG GGGCCGTATA AAGTAAGCAA 801 AGGAATCAAA CCGACGGAAG GATTGATGGT CGATTTCTCC GATATCCAAC 851 CATACGGCAA TCATACGGGT AACTCCGCCC CATCCGTAGA GGCTGATAAC 901 AGTCATGAGG GGTATGGATA CAGCGATGAA GCAGTGCGAC AACATAGACA 951 AGGGCAACCT TGA This corresponds to the amino acid sequence <SEQ ID 1326; ORF 406>: g406.pep 1 MRARLLIPIL FSVFILSACG TLTGIPSHGG GKRFAVEQEL VAASARAAVK 51 DMDLQALHGR KVALYIATMG DQGSGSLTGG RYSIDALIRG EYINSPAVRT 101 DYTYPRYETT AETTSGGLTG LTTSLSTLNA PALSRTQSDG SGSRSSLGLN 151 IGGMGDYRNE TLTTNPRDTA FLSHLVQTVF FLRGIDWSP ANADTDVFIN 201 IDVFGTIRNR TEMHLYNAET LKAQTKLEYF AVDRTNKKLL IKPKTNAFEA 251 AYKENYALWM GPYKVSKGIK PTEGLMVDFS DIQPYGNHTG NSAPSVEADN 301 SHEGYGYSDE AVRQHRQGQP * The following partial DNA sequence was identified in N meningitidis <SEQ ID 1327>: m406.seq 1 ATGCAAGCAC GGCTGCTGAT ACCTATTCTT TTTTCAGTTT TTATTTTATC 51 CGCCTGCGGG ACACTGACAG GTATTCCATC GCATGGCGGA GGTAAACGCT 101 TTGCGGTCGA ACAAGAACTT GTGGCCGCTT CTGCCAGAGC TGCCGTTAAA 151 GACATGGATT TACAGGCATT ACACGGACGA AAAGTTGCAT TGTACATTGC 201 CACTATGGGC GACCAAGGTT CAGGCAGTTT GACAGGGGGT CGCTACTCCA 251 TTGATGCACT GATTCGTGGC GAATACATAA ACAGCCCTGC CGTCCGTACC 301 GATTACACCT ATCCACGTTA CGAAACCACC GCTGAAACAA CATCAGGCGG 351 TTTGACAGGT TTAACCACTT CTTTATCTAC ACTTAATGCC CCTGCACTCT 401 CTCGCACCCA ATCAGACGGT AGCGGAAGTA AAAGCAGTCT GGGCTTAAAT 451 ATTGGCGGGA TGGGGGATTA TCGAAATGAA ACCTTGACGA CTAACCCGCG 501 CGACACTGCC TTTCTTTCCC ACTTGGTACA GACCGTATTT TTCCTGCGCG 551 GCATAGACGT TGTTTCTCCT GCCAATGCCG ATACAGATGT GTTTATTAAC 601 ATCGACGTAT TCGGAACGAT ACGCAACAGA ACCGAAATGC ACCTATACAA 651 TGCCGAAACA CTGAAAGCCC AAACAAAACT GGAATATTTC GCAGTAGACA 701 GAACCAATAA AAAATTGCTC ATCAAACCAA AAACCAATGC GTTTGAAGCT 751 GCCTATAAAG AAAATTACGC ATTGTGGATG GGGCCGTATA AAGTAAGCAA 801 AGGAATTAAA CCGACGGAAG GATTAATGGT CGATTTCTCC GATATCCGAC 851 CATACGGCAA TCATACGGGT AACTCCGCCC CATCCGTAGA GGCTGATAAC 901 AGTCATGAGG GGTATGGATA CAGCGATGAA GTAGTGCGAC AACATAGACA 951 AGGACAACCT TGA This corresponds to the amino acid sequence <SEQ ID 1328; ORF 406>: m406. ep 1 MQARLLIPIL FSVFILSACG TLTGIPSHGG GKRFAVEQEL VAASARAAVK 51 DMDLQALHGR KVALYIATMG DQGSGSLTGG RYSIDALIRG EYINSPAVRT 101 DYTYPRYETT AETTSGGLTG LTTSLSTLNA PALSRTQSDG SGSKSSLGLN 151 IGGMGDYRNE TLTTNPRDTA FLSHLVQTVF FLRGIDWSP ANADTDVFIN 201 IDVFGTIRNR TEMHLYNAET LKAQTKLEYF AVDRTNKKLL IKPKTNAFEA 251 AYKENYALWM GPYKVSKGIK PTEGLMVDFS DIRPYGNHTG NSAPSVEADN 301 SHEGYGYSDE WRQHRQGQP * Computer analysis of the amino acid sequences gave the following results: Homology with a predicted ORF from N meningitidis menA with menB ORF 406 shows 98.8% identity over a 320 aa overlap with a predicted ORF (ORF406.a) from N gonorrhoeae: g406/m406 10 20 30 40 50 60 g406. pep MRARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAASARAAVKDMDLQALHGR 1 : 1111111 I M I M I M 111111 M 1111 M 1111111111111 M 111111111 II 11 m406 MQARLLIPI FSVFILSACGTLTGIPSHGGGKRFAVEQELVAASARAAVKDMDLQALHGR 10 20 30 40 50 60 70 80 90 100 110 120 g406. pep KVALYIATMGDQGSGSLTGGRYSIDALIRGEYIΝSPAVRTDYTYPRYETTAETTSGGLTG MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM m406 KVALYIATMGDQGSGSLTGGRYSIDALIRGEYIΝSPAVRTDYTYPRYETTAETTSGGLTG 70 80 90 100 110 120 130 140 150 160 170 180 g406. pep LTTSLSTLΝAPALSRTQSDGSGSRSSLGLΝIGGMGDYRΝETLTTΝPRDTAFLSHLVQTVF MIMMMMMMMMIMhMMMMMMIMMMMIMMMMMMM m406 LTTSLSTLΝAPALSRTQSDGSGSKSSLGLΝIGGMGDYRΝETLTTΝPRDTAFLSHLVQTVF 130 140 150 160 170 180 190 200 210 220 230 240 g406 . pep FLRGIDWSPAΝADTDVFIΝIDVFGTIRΝRTEMHLYΝAETLKAQTKLEYFAVDRTΝKKLL M I M M I M M M M I M M M M M M M M M M M M M M M I M M M M I I M m406 FLRGIDWSPAΝADTDVFIΝIDVFGTIRΝRTEMHLYΝAETLKAQTKLEYFAVDRTΝKKLL 190 200 210 220 230 240 250 260 270 280 290 300 g406 . pep IKPKTΝAFEAAYKEΝYALWMGPYKVSKGIKPTEGLMVDFSDIQPYGΝHTGΝSAPSVEADΝ MMMMMMMMMMMIMMMMMMMMMIMMMMIMIMMM m406 IKPKTΝAFEAAYKEΝYALWMGPYKVSKGIKPTEGLMVDFSDIRPYGΝHTGΝSAPSVEADΝ 250 260 270 280 290 300 310 320 g406. pep SHEGYGYSDEAVRQHRQGQPX I M I I I I I I h l l l M I M M m406 SHEGYGYSDEWRQHRQGQPX 310 320 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1329>: a406. seq 1 ATGCAAGCAC GGCTGCTGAT ACCTATTCTT TTTTCAGTTT TTATTTTATC 51 CGCCTGCGGG ACACTGACAG GTATTCCATC GCATGGCGGA GGTAAACGCT 101 TCGCGGTCGA ACAAGAACTT GTGGCCGCTT CTGCCAGAGC TGCCGTTAAA 151 GACATGGATT TACAGGCATT ACACGGACGA AAAGTTGCAT TGTACATTGC 201 AACTATGGGC GACCAAGGTT CAGGCAGTTT GACAGGGGGT CGCTACTCCA 251 TTGATGCACT GATTCGTGGC GAATACATAA ACAGCCCTGC CGTCCGTACC 301 GATTACACCT ATCCACGTTA CGAAACCACC GCTGAAACAA CATCAGGCGG 351 TTTGACAGGT TTAACCACTT CTTTATCTAC ACTTAATGCC CCTGCACTCT 401 CGCGCACCCA ATCAGACGGT AGCGGAAGTA AAAGCAGTCT GGGCTTAAAT 451 ATTGGCGGGA TGGGGGATTA TCGAAATGAA ACCTTGACGA CTAACCCGCG 501 CGACACTGCC TTTCTTTCCC ACTTGGTACA GACCGTATTT TTCCTGCGCG 551 GCATAGACGT TGTTTCTCCT GCCAATGCCG ATACGGATGT GTTTATTAAC 601 ATCGACGTAT TCGGAACGAT ACGCAACAGA ACCGAAATGC ACCTATACAA 651 TGCCGAAACA CTGAAAGCCC AAACAAAACT GGAATATTTC GCAGTAGACA 701 GAACCAATAA AAAATTGCTC ATCAAACCAA AAACCAATGC GTTTGAAGCT 751 GCCTATAAAG AAAATTACGC ATTGTGGATG GGACCGTATA AAGTAAGCAA 801 AGGAATTAAA CCGACAGAAG GATTAATGGT CGATTTCTCC GATATCCAAC 851 CATACGGCAA TCATATGGGT AACTCTGCCC CATCCGTAGA GGCTGATAAC 901 AGTCATGAGG GGTATGGATA CAGCGATGAA GCAGTGCGAC GACATAGACA 951 AGGGCAACCT TGA This corresponds to the amino acid sequence <SEQ ID 1330; ORF 406.a>: a406.ρep 1 MQARLLIPIL FSVFILSACG TLTGIPSHGG GKRFAVEQEL VAASARAAVK 51 DMDLQALHGR KVALYIATMG DQGSGSLTGG RYSIDALIRG EYINSPAVRT 101 DYTYPRYETT AETTSGGLTG LTTSLSTLNA PALSRTQSDG SGSKSSLGLN 151 IGGMGDYRNE TLTTNPRDTA FLSHLVQTVF FLRGIDWSP ANADTDVFIN 201 IDVFGTIRNR TEMHLYNAET LKAQTKLEYF AVDRTNKKLL IKPKTNAFEA 251 AYKENYALWM GPYKVSKGIK PTEGLMVDFS DIQPYGNHMG NSAPSVEADN 301 SHEGYGYSDE AVRRHRQGQP * m406/a406 98.8% identity in 320 aa overlap 10 20 30 40 50 60 m406 .pep MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAASARAAVKDMDLQALHGR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a406 MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAASARAAVKDMDLQALHGR 10 20 30 40 50 60 70 80 90 100 110 120 m406.pep KVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDYTYPRYETTAETTSGGLTG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a406 KVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDYTYPRYETTAETTSGGLTG 70 80 90 100 110 120 130 140 150 160 170 180 m406.pep LTTSLSTLNAPALSRTQSDGSGSKSSLGLNIGGMGDYRNETLTTNPRDTAFLSHLVQTVF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a406 LTTSLSTLNAPALSRTQSDGSGSKSSLGLNIGGMGDYRNETLTTNPRDTAFLSHLVQTVF 130 140 150 160 170 180 190 200 210 220 230 240 m406 . pep FLRGIDWSPANADTDVFINIDVFGTIRNRTEMHLYNAETLKAQTKLEYFAVDRTNKKLL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a406 FLRGIDWSPANADTDVFINIDVFGTIRNRTEMHLYNAETLKAQTKLEYFAVDRTNKKLL 190 200 210 220 230 240 250 260 270 280 290 300 m406 . pep IKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLMVDFSDIRPYGNHTGNSAPSVEADN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I a 06 IKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLMVDFSDIQPYGNHMGNSAPSVEADN 250 260 270 280 290 300 310 320 m406.pep SHEGYGYSDEWRQHRQGQPX I I I I I I I I I I : I I : I II I I I I a406 SHEGYGYSDEAVRRHRQGQPX 310 320 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1331>: g501.seq 1 atggtcggac ggaccttgac cgcagatacc gacatatttg ttctgcttgc, 51 ggcaggcgga gatggcaaga tgcagcatca ctttgacggc agggttgcgt 101 tcgtcaaacg attcggacac caagccgctg tctcggtcga ggccgagggt 151 cagctgggtc atgtcgttcg agccgatgga gaagccgtcg aagtattgca 201 ggaattgttc cgccaatacc gcgttgctcg gcagctcgca catcataatc 251 aggcgcaggc cgtttttgcc gcgttccaag ccgttttctt tcaatgcctt 301 aaccactgct tcggcttcgc ccaaagtgcg gacgaacgga atcatgattt 351 cgacgttggt cagacccatt tcgtcacgaa cgcgtttcaa ggctttgcat 401 tccaaggcga aacagtcttt gaagctctcg gcaacataac gcgccgcacc 451 acggaagccc aacatcgggt tttcttcatg cggttcgtat acgctgccgc 501 cgaccaggtt ggcgtattcg ttggatttga agtcggacat acggacgatg 551 gttttacgcg gataaaccga tgcggcaagc gttgccacgc cttcggcgat 601 tttatcgacg tagaagtcga caggggatgc gtaaccggcg atgcggcgga 651 taatttccgc tttcagttcg tcgtcttgtt tgtcaaattc caacaaggct 701 ttcgggtgga tgccgatttg gcggttgatg ataaattcca tacgcgccaa 751 gccgatgcct tcgctgggca gattggcgaa gctgaatgcg agttcgggat 801 tgccgacgtt catcatgact ttgacgggtg cttttggcat attgtccaag 851 gcgacatcgg taatttgtac gtccagcagg ccggcataga taaagccggt 901 atcgccttcg gcacaggata cggtaacttc ctgaccgttt tccaagagtt 951 cggtcgcatt gccgcagccg acgacggcag gaatacccag ttcgcgcgcg 1001 atgatggcgg cgtggcaggt gcgtccgccg cggttggtca cgatggcgga 1051 agcacgtttc atcacgggtt cccaatccgg atcggtcatg tcggtaacca 1101 gtacgtcgcc ggcttcgacg gaatccatct cggaagcatc tttaatcagg 1151 cgcaccttgc cctgaccgac tttttgaccg atggcacgac cttcgcacaa 1201 gacggttttt tcgccgttga tggcgtagcg gcgcaggttg cggctgcctt 1251 cttcttggga tttgacggtt tcggggcggg cttgcaggat gtagagtttg 1301 ccgtccaggc cgtcgcgtcc ccattcgata tccatcgggc ggccgtagtg 1351 tttttcgatg gtcagcgcgt agtgtgccaa ctcggtgatt tcttcgtcgg 1401 taatggagaa gcggttgcgg tcttcttcgg ggacttcgac gttggttacc 1451 gatttgccgg cttcggcttt gtcggtgaaa atcattttga tgtgtttcga 1501 acccatggtc ttgcgcagga tggcgggttt gcctgctttg agcgtgggtt 1551 tgaacacata aaattcgtcc gggttgaccg cgccttgtac gacgttttcg 1601 cccagaccgt aagaggaggt aacaaagacg acttggttgt agccggattc 1651 ggtgtcgagg gtgaacatca cacctga This corresponds to the amino acid sequence <SEQ ID 1332; ORF 501.ng>: g501.pep 1 MVGRTLTADT DIFVLLAAGG DGKMQHHFDG RVAFVKRFGH QAAVSVEAEG 51 QLGHWRADG EAVEVLQELF RQYRVARQLA HHNQAQAVFA AFQAVFFQCL 101 NHCFGFAQSA DERNHDFDVG QTHFVTNAFQ GFAFQGETVF EALGNITRRT 151 TEAQHRVFFM RFVYAAADQV GVFVGFEVGH TDDGFTRINR CGKRCHAFGD 201 FIDVEVDRGC VTGDAADNFR FQFWLFVKF QQGFRVDADL AVDDKFHTRQ 251 ADAFAGQIGE AECEFGIADV HHDFDGCFWH IVQGDIGNLY VQQAGIDKAG 301 IAFGTGYGNF LTVFQEFGRI AAADDGRNTQ FARDDGGVAG ASAAVGHDGG 351 STFHHGFPIR IGHVGNQYVA GFDGIHLGSI FNQAHLALTD FLTDGTTFAQ 401 DGFFAVDGVA AQVAAAFFLG FDGFGAGLQD VEFAVQAVAS PFDIHRAAW 451 FFDGQRWCQ LGDFFVGNGE AVAVFFGDFD VGYRFAGFGF VGENHFDVFR 501 THGLAQDGGF ACFERGFEHI KFVRVDRALY DVFAQTVRGG NKDDLWAGF 551 GVEGEHHT* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1333>: m501. seq 1 atggtcggac sggccttgac cgcagatgcc gacatatttg ttctgcttgc 51 ggcaggcgga gatggcaagg tgcagcatca ctttgacggc agggttgcgt 101 tcgtcaaacg attcggatac caagccgctg tcgcggtcga gaccgagggt 151 cagttgggtc atgtcgttcg agccgatgga gaagccgtcg aagtattgca 201 ggaattgttc cgccaatacc gcgttgctcg gcagctcgca catcataatc 251 aggcgcaggc cgtttttgcc gcgttccaag ccgttttctt tcagggcttt 301 gacaacggmt tcggcttcgc ccaaagtgcg gacgaacgga atcatgattt 351 caacgttggy caaccccatt tcatcgcgga cgcgtttcaa ggctttgcat 401 tccaaggcga aacagtcttt gaagttgtcg gcgacataac gcgccgcacc 451 acggaagccc aacatcgggt tttcttcatg cggttcgtat acgttgccgc 501 cgaccaggtt ggcgtattcg ttggatttga agtcggacat acggacgatg 551 gttttacgcg gataaaccga tgcggccaat gtcgccacgc cttcggcgat 601 tttatcgacg tagaagtcga caggggacgc gtaaccggcg atacggcggg 651 taatttccgc ttttaattcg tcgtcttgtt tgtcaaattc caacaargct 701 ttggggtgga taccgatttg gcggttgatg ataaattcca tacgcgccaa 751 gccgatgcct tcgctgggca ggttggcgaa gctgaatgcg agttcgggat 801 tgccgacgtt catcatgact tttacaggtg ctttaggcat attgtctaag 851 gcgacatcgg taatctgtac gtccaacaga ccggcataga taaagccggt 901 atcgccttcg gcacaggata cggtaacttc ttgaccgttt ttcagcaatt 951 cggttgcatt gccgcagccg acaacggcag gaatgcccaa ttcacgcgcg 1001 atgatggcgg cgtggcaggt acggccgccg cggttggtaa cgatggcaga 1051 agcacgtttc atcacgggtt cccaatccgg atcggtcatg tcggtaacga 1101 gtacgtcgcc ggcttcgacg gaatccatct cggaagcatc tttaatcagg 1151 cgcaccttgc cctgaccgac tttctgaccg atggcgcggc cttcgcataa 1201 tacggttttg tcgccgttga tggcgaagcg gcgcaggttg cggttgccct 1251 cttcttggga ttttacggtt tcgggacggg cttgcaggat gtagagtttg 1301 ccgtccaagc cgtcgcgtcc ccattcgata tccatcgggc ggccgtagtg 1351 tttttcgatg gtcagtgcgt aatgcgccaa ctcagtaatt tcttcgtcgg 1401 taatggagaa gcggttgcgg tcttcctcgg ggacatcgac gttggttacg 1451 gatttaccgg cttctgcttt gtcggtaaaa atcattttga tgtgttttga 1501 acccatggtt ttacgcagga tggcgggctt gcccgytttg agcgtgggtt 1551 tgaacacatr aaattcgtcc gggttgaccg caccttgtac gacgttttcg 1601 cccagaccgt aagaggaggt aacaaagacg acytgatcgt akccggattc 1651 ggtgtcgagg gtgaacatca cacctga This corresponds to the amino acid sequence <SEQ ID 1334; ORF 501>: m501.pep 1 MVGXALTADA DIFVLLAAGG DGKVQHHFDG RVAFVKRFGY QAAVAVETEG 51 QLGHWRADG EAVEVLQELF RQYRVARQLA HHNQAQAVFA AFQAVFFQGF 101 DNGFGFAQSA DERNHDFNVG QPHFIADAFQ GFAFQGETVF EWGDITRRT 151 TEAQHRVFFM RFVYVAADQV GVFVGFEVGH TDDGFTRINR CGQCRHAFGD 201 FIDVEVDRGR VTGDTAGNFR FXFWLFVKF QQXFGVDTDL AVDDKFHTRQ 251 ADAFAGQVGE AECEFGIADV HHDFYRCFRH IVXGDIGNLY VQQTGIDKAG 301 IAFGTGYGNF LTVFQQFGCI AAADNGRNAQ FTRDDGGVAG TAAAVGNDGR 351 STFHHGFPIR IGHVGNEYVA GFDGIHLGSI FNQAHLALTD FLTDGAAFAX 401 YGFVAVDGEA AQVAVALFLG FYGFGTGLQD VEFAVQAVAS PFDIHRAAW 451 FFDGQCVMRQ LSNFFVGNGE AVAVFLGDID VGYGFTGFCF VGKNHFDVFX 501 THGFTQDGGL ARFERGFEHX KFVRVDRTLY DVFAQTVRGG NKDDLIVXGF 551 GVEGEHHT* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 501 shows 86.2% identity over a 558 aa overlap with a predicted ORF (ORF 501. ng) from N gonorrhoeae: m501/g501 10 20 30 40 50 60 m501. ep MVGXALTADADIFVLLAAGGDGKVQHHFDGRVAFVKRFGYQAAVAVETEGQLGHWRADG M l g501 MVGRTLTADTDIFVLLAAGGDGKMQHHFDGRVAFVKRFGHQAAVSVEAEGQLGHWRADG 10 20 30 40 50 60 70 80 90 100 110 120 m501. pep EAVEVLQELFRQYRVARQLAHHΝQAQAVFAAFQAVFFQGFDΝGFGFAQSADERΝHDFΝVG M I M M M M M M I I M M M M M M M I M M M ■ M M M M M M I h M g501 EAVEVLQELFRQYRVARQLAHHΝQAQAVFAAFQAVFFQCLΝHCFGFAQSADERΝHDFDVG 70 80 90 100 110 120 130 140 150 160 170 180 m501 . pep QPHFIADAFQGFAFQGETVFEWGDITRRTTEAQHRVFFMRFVYVAADQVGVFVGFEVGH I M - M M M M M M M h M M M M M M M M I M I I h M M M M I M M M g501 QTHFVTΝAFQGFAF Q GETVFEALGΝITRRTTEAQHRVFFMRFVYAAADQVGVFVGFEVGH 130 140 150 160 170 180 190 200 210 220 230 240 m501. pep TDDGFTRIΝRCG Q CRHAFGDFIDVEVDRGRVTGDTAGΝFRFXFWLFVKFQQXFGVDTDL g501 TDDGFTRINRCGKRCHAFGDFIDVEVDRGCVTGDAADNFRFQFWLFVKFQQGFRVDADL 190 200 210 220 230 240 250 260 270 280 290 300 m501.pep AVDDKFHTRQADAFAGQVGEAECEFGIADVHHDFYRCFRHIVXGDIGNLYVQQTGIDKAG II Ml MMMMMMIMM g501 AVDDKFHTRQADAFAGQIGEAECEFGIADVHHDFDGCFWHIVQGDIGNLYVQQAGIDKAG 250 260 270 280 290 300 310 320 330 340 350 360 m501. pep IAFGTGYGNFLTVFQQFGCIAAADNGRNAQFTRDDGGVAGTAAAVGNDGRSTFHHGFPIR MMIMMMMIhM II II 1 = II I M I = I II II II h M II I M I g501 IAFGTGYGNFLTVFQEFGRIAAADDGRNTQFARDDGGVAGASAAVGHDGGSTFHHGFPIR 310 320 330 340 350 360 370 380 390 400 410 420 mδOl.pep IGHVGNEYVAGFDGIHLGSIFNQAHLALTDFLTDGAAFAXYGFVAVDGEAAQVAVALFLG MMMMMMMMMMMMIMMMMIhMI II MM IIIMMMM g501 IGHVGNQYVAGFDGIHLGSIFNQAHLALTDFLTDGTTFAQDGFFAVDGVAAQVAAAFFLG 370 380 390 400 410 420 430 440 450 460 470 480 m501. pep FYGFGTGLQDVEFAVQAVASPFDIHRAAWFFDGQCVMRQLSNFFVGNGEAVAVFLGDID I MIMMMMMMMIMMMMMMIM h I h M I II I II II II I = II = I g501 FDGFGAGLQDVEFAVQAVASPFDIHRAAWFFDGQRWCQLGDFFVGNGEAVAVFFGDFD 430 440 450 460 470 480 490 500 510 520 530 540 m501. pep VGYGFTGFCFVGKNHFDVFXTHGFTQDGGLARFERGFEHXKFVRVDRTLYDVFAQTVRGG Ml IMI IIIMIMM MhMllhl g501 VGYRFAGFGFVGENHFDVFRTHGLAQDGGFACFERGFEHIKFVRVDRALYDVFAQTVRGG 490 500 510 520 530 540 550 m501.pep ΝKDDLIVXGFGVEGEHHT 1 111111111 ■• 11 llllllllll llll g501 ΝKDDL AGFGVEGEHHT 550 allowing partial DNA sequence was identified in N meningitidis <SEQ ID 1335: a501. seq (partial) 1 ATGGTCGGAC GGGCCTTGAC CGCAGATGCC GACATATTTG TTCTGCTTGC 51 GGCAGGCGGA GATGGCAAGG TGCAGCATCA CTTTGACGGC AGGGTTGCGT 101 TCGTCAAACG ATTCGGATAC CAAGCCGCTG TCGCGGTCGA GACCGAGGGT 151 CAGTTGGGTC ATGTCGTTCG AGCCGATGGA GAAGCCGTCG AAGTATTGCA 201 GGAATTGTTC CGCCAATACC GCGTTGCTCG GCAGCTCGCA CATCATAATC 251 AGGCGCAGGC CGTTTTTGCC GCGTTCCAAG CCGTTTTCTT TCAGGGCTTT 301 GACAACGGCT TCGGCTTCGC CCAAAGTGCG GACGAACGGA ATCATGATTT 351 CAACGTTGGT CAACCCCATT TCATCGCGGA CGCGTTTCAA GGCTTTGCAT 401 TCCAAGGCGA AACAGTCTTT GAAGTTGTCG GCGACATAAC GCGCCGCACC 451 ACGGAAGCCC AACATCGGGT TTTCTTCATG CGGTTCGTAT ACGTTGCCGC 501 CGACCAGGTT GGCGTATTCG TTGGATTTGA AGTCGGACAT ACGGACGATG 551 GTTTTACGCG GATAAACCGA TGCGGCCAAT GTCGCCACGC CTTCGGCGAT 601 TTTATCGACG TAGAAGTCGA CAGGGGACGC GTAACCGGCG ATACGGCGGG 651 TAATTTCCGC TTTTAATTCG TCGTCTTGTT TGTCAAATTC CAACAAGGCT 701 TTGGGGTGGA TACCGATTTG GCGGTTGATG ATAAATTCCA TACGCGCCAA 751 GCCGATGCCT TCGCTGGGCA GGTTGGCGAA GCTGAATGCG AGTTCGGGAT 801 TGCCGACGTT CATCATGACT TTTACAGGTG CTTTAGGCAT GTTGTCCAAA 851 GCAACATCGG TAATTTGTAC GTCCAGCAGG CCGGAGTAGA TGAAGCCGGT 901 ATCGCCTTCG GCACAGGATA CGGTAACTTC TTGACCGTTT TTCAGCAATT 951 CGGTTGCATT GCCGCAGCCG ACAACGGCAG GAATACCCAG TTCGCGCGCG 1001 ATGATGGCGG CGTGGCAGGT ACGTCCGCCC CTGTTGGTCA CGATGGCGGA 1051 AGCGCGTTTC ATCACCGGTT CCCAATCTGG GTCGGTCATG TCGGTAACCA 1101 GTACGTCGCC GGCTTCGACG GAATCCATCT CGGAAGCATC TTTAATCAGG 1151 CGTACCTTGC CCTGACCGAC TTTCTGACCG ATGGCGCGGC CTTCGCACAA 1201 GACGGTTTTT TCGCCGTTGA TAGAAAAGCG GCGCAGGTTG CGGCTGCCTT 1251 CTTCCTGGGA TTTGACGGTT TCGGGACGGG CTTGCAGGAT GTAGAGTTTG 1301 CCGTCCAAGC CGTCGCGTCC CCATTCGATG TCCATCGGGC GGCCGTAGTG 1351 TTTTTCGATG GTCAGTGCGT AATGCGCCAA CTCGGTGATT TCTTCGTCGG 1401 TAATGGAGAA GCGGTTGCGG TCTTCTTCGG GGACATCGAC GTTGGTTACC 1451 GATTTGCCGG CTTCTGCTTT GTCGGTAAAA ATCATTTTGA TGTGTTTTGA 1501 GCCCATGGTT TTGCGCAGGA TGGCAGGTTT GCCTGCTTTC AGCGTGGGTT 1551 TGAACACATA GAATTCGTCG GGATTGACTG CGCCTTGTAC GACGTTTTCG 1601 CCCAGACCGT AGGATGAAGT GACAAAGACG ACTTGGTCGT AACCGGATTC 1651 GGTATCGAGG GTGAACATCA C This corresponds to the amino acid sequence <SEQ ID 1336; ORF 501. a>: a501.pep 1 MVGRALTADA DIFVLLAAGG DGKVQHHFDG RVAFVKRFGY QAAVAVETEG 51 QLGHWRADG EAVEVLQELF RQYRVARQLA HHNQAQAVFA AFQAVFFQGF 101 DNGFGFAQSA DERNHDFNVG QPHFIADAFQ GFAFQGETVF EVVGDITRRT 151 TEAQHRVFFM RFVYVAADQV GVFVGFEVGH TDDGFTRINR CGQCRHAFGD 201 FIDVEVDRGR VTGDTAGNFR F*FVVLFVKF QQGFGVDTDL AVDDKFHTRQ 251 ADAFAGQVGE AECEFGIADV HHDFYRCFRH VVQSNIGNLY VQQAGVDEAG 301 IAFGTGYGNF LTVFQQFGCI AAADNGRNTQ FARDDGGVAG TSAPVGHDGG 351 SAFHHRFPI VGHVGNQYVA GFDGIHLGSI FNQAYLALTD FLTDGAAFAQ 401 DGFFAVDRKA AQVAAAFFLG FDGFGTGLQD VEFAVQAVAS PFDVHRAAVV 451 FFDGQCVMRQ LGDFFVGNGE AVAVFFGDID VGYRFAGFCF VGKNHFDVF* 501 AHGFAQDGRF ACFQRGFEHI EFVGIDCALY DVFAQTVG*S DKDDLVVTGF 551 GIEGEHH m501/a501 90.3% identity in 557 aa overlap 10 20 30 40 50 60 m501.pep MVGXALTADADIFVLLAAGGDGKVQHHFDGRVAFVKRFGYQAAVAVETEGQLGHVVRADG III I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a501 MVGRALTADADIFVLLAAGGDGKVQHHFDGRVAFVKRFGYQAAVAVETEGQLGHVVRADG 10 20 30 40 50 60 70 80 90 100 110 120 m501. .pep EAVEVLQELFRQYRVARQLAHHNQAQAVFAAFQAVFFQGFDNGFGFAQSADERNHDFNVG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a501 EAVEVLQELFRQYRVARQLAHHNQAQAVFAAFQAVFFQGFDNGFGFAQSADERNHDFNVG 70 80 90 100 110 120 130 140 150 160 170 180 m501. .pep QPHFIADAFQGFAFQGETVFEVVGDITRRTTEAQHRVFFMRFVYVAADQVGVFVGFEVGH I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a501 QPHFIADAFQGFAFQGETVFEVVGDITRRTTEAQHRVFFMRFVYVAADQVGVFVGFEVGH 130 140 150 160 170 180 190 200 210 220 230 240 m501. ,pep TDDGFTRINRCGQCRHAFGDFIDVEVDRGRVTGDTAGNFRFXFVVLFVKFQQXFGVDTDL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I lllllll a501 TDDGFTRINRCGQCRHAFGDFIDVEVDRGRVTGDTAGNFRFXFVVLFVKFQQGFGVDTDL 190 200 210 220 230 240 250 260 270 280 290 300 πι501. .pep AVDDKFHTRQADAFAGQVGEAECEFGIADVHHDFYRCFRHIVXGDIGNLYVQQTGIDKAG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I : :| I I I I I I I : h I : I I a501 AVDDKFHTRQADAFAGQVGEAECEFGIADVHHDFYRCFRHVVQSNIGNLYVQQAGVDEAG 250 260 270 280 290 300 310 320 330 340 350 360 m501. .pep IAFGTGYGNFLTVFQQFGCIAAADNGRNAQFTRDDGGVAGTAAAVGNDGRSTFHHGFPIR I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I h I I I I I I I I h I I I :| I hill III a501 IAFGTGYGNFLTVFQQFGCIAAADNGRNTQFARDDGGVAGTSAPVGHDGGΞAFHHRFPIW 310 320 330 340 350 360 370 380 390 400 410 420 m501.pep IGHVGNEYVAGFDGIHLGSIFNQAHLALTDFLTDGAAFAXYGFVAVDGEAAQVAVALFLG : I I I I I : I I I I I I I I I I I I I I I I h I I 1 I I I I I I I I I I I II III : I I I I h I : I I I a501 VGHVGNQYVAGFDGIHLGSIFNQAYLALTDFLTDGAAFAQDGFFAVDRKAAQVAAAFFLG 370 380 390 400 410 420 430 440 450 460 470 480 m501.pep FYGFGTGLQDVEFAVQAVASPFDIHRAAVVFFDGQCVMRQLΞNFFVGNGEAVAVFLGDID I I I I I I I I I I I I I I I I I I I I I I: I I I I I I I I I I I I I I I I I ::| I I I I I I I I I I hi I I I a501 FDGFGTGLQDVEFAVQAVASPFDVHRAAVVFFDGQCVMRQLGDFFVGNGEAVAVFFGDID 430 440 450 460 470 480 490 500 510 520 530 540 m501. pep VGYGFTGFCFVGKNHFDVFXTHGFTQDGGLARFERGFEHXKFVRVDRTLYDVFAQTVRGG III I :| I I I I I I I I I I I I I : I I I : I I I : I I : I I I I I : I I : I .'lllllllll : a501 VGYRFAGFCFVGKNHFDVFXAHGFAQDGRFACFQRGFEHIEFVGIDCALYDVFAQTVGXS 490 500 510 520 530 540 550 559 m501. pep NKDDLIVXGFGVEGEHHTX : I I I h h I I I :| I I I I a501 DKDDLVVTGFGIEGEHH 550 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1337>: g502.seq 1 atgatgaaac cgcacaacct gttccaattc ctcgccgttt gctccctgac 51 cgtcgccgtc gcttccgcac aggcgggcgc ggtggacgcg ctcaagcaat 101 tcaacaacga tgccgacggt atcagcggca gcttcaccca aaccgtccaa 151 agcaaaaaga aaacccaaac cgcgcacggc acgttcaaaa tcctgcgccc 201 gggcctcttc aaatgggaat acactttgcc ctacagacag actattgtcg 251 gcgacggtca aaccgtttgg ctctacgatg ttgatttggc acaagtgacc 301 aagtcgtccc aagaccaggc catcggcggc agccccgccg ccatcctgtc 351 gaacaaaacc gccctcgaaa gcagttacac gctgaaagag gacggttcgt 401 ccaacggcat cgattatgtg cggggcaacg cccaaacgca acaacgccgg 451 ctaccaatac atccgcatcg gcttcaaagg cggcaacctc gccgccatgc 501 agcttaa This corresponds to the amino acid sequence <SEQ ID 1338; ORF 502.ng>: g502.pep 1 MMKPHΝLFQF LAVCSLTVAV ASAQAGAVDA LKQFΝΝDADG ISGSFTQTVQ 51 SKKKTQTAHG TFKILRPGLF KWEYTLPYRQ TIVGDGQTVW LYDVDLAQVT 101 KSSQDQAIGG SPAAILSΝKT ALESSYTLKE DGSSΝGIDYV RGΝAQTQQRR 151 LPIHPHRLQR RQPRRHAA* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1339>: m502. seq 1 atgatgaaac cgcacaacct gttccaattc ctcgccgttt gctccctgac 51 cgtcgccgtc gcttccgcac aggcgggcgc ggtagacgcg cttaagcaat 101 tcaacaacga tgccgacggt atcagcggca gcttcaccca amccgtccaa 151 gcaaaaaga aaacccaaac cgcgcacggc acgttcaaaa tcctgcgacc 201 gggccttttc aaatgggaat acaccaaact t.acaggcaa accatcgtcg 251 gcgacggtca aacygtttgg ctmtacgatg tygatctggc acaagtgacc 301 aagtcgtccc aagaccaggc cataggcgsc agccccgccg ccatcctgtc 351 gaacaaarcc gccctcgaaa gcagctacac gctgaaagag gacggttcgt 401 ccaacggcat cgattatgtg ggcaacgccc aaacgcaaca acgccggcta 451 ccaatacatc cgcatcggct tcaaaggcgg caacctcgcc gccatgcagc 501 tyaa This corresponds to the amino acid sequence <SEQ ID 1340; ORF 502.ng>: m502.pep 1 MMKPHΝLFQF LAVCSLTVAV ASAQAGAVDA LKQFΝΝDADG ISGSFTQXVQ 51 XKKKTQTAHG TFKILRPGLF KWEYTKLYRQ TIVGDGQTVW LYDVDLAQVT 101 KSSQDQAIGX SPAAILSΝKX ALESSYTLKE DGSSΝGIDYV GΝAQTQQRRL 151 PIHPHRLQRR QPRRHAAX Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 502 shows 95.8% identity over a 168 aa overlap with a predicted ORF (ORF 502.ng) from N gonorrhoeae: m502/g502 10 20 30 40 50 60 m502. pep MMKPHΝLFQFLAVCSLTVAVASAQAGAVDALKQFΝΝDADGISGSFTQXVQXKKKTQTAHG MMMMMMMMMMIMMMMMMMMMMMMIhM IMIMIII g502 MMKPHΝLFQFLAVCSLTVAVASAQAGAVDALKQFΝΝDADGISGSFTQTVQSKKKTQTAHG 10 20 30 40 50 60 70 80 90 100 110 120 m50 . pep TFKILRPGLFKWEYTKLYRQTIVGDGQTVWLYDVDLAQVTKSSQDQAIGXSPAAILSΝKX MMIMMMMM II II I II II II II II 11 II I II I II II II II I g502 TFKILRPGLFKWEYTLPYRQTIVGDGQTVWLYDVDLAQVTKSSQDQAIGGSPAAILSΝKT 70 80 90 100 110 120 130 140 150 160 m502.pep ALESSYTLKEDGSSΝGIDYV-GΝAQTQQRRLPIHPHRLQRRQPRRHAA MMIMMMIMMMM I II M II II M 111 II II 11 M I II M g502 ALESSYTLKEDGSSΝGIDYVRGΝAQTQQRRLPIHPHRLQRRQPRRHAA 130 140 150 160 The following partial DΝA sequence was identified inN. meningitidis <SEQ ID 1341>: a502. seq 1 ATGATGAAAC CGCACAACCT GTTCCAATTC CTCGCCGTTT GCTCCCTGAC 51 CGTCTCCGTC GCTTCCGCAC AGGCGGGCGC GGTGGACGCG CTCAAGCAAT 101 TCAACAACGA TGCCGACGGT ATCAGCGGCA GCTTCACCCA AACCGTCCAA 151 AGCAAAAAGA AAACCCAAAC CGCGCACGGC ACGTTCAAAA TCCTGCGCCC 201 GGGCCTCTTT AAATGGGAAT ACACTTCGCC TTACAAACAG ACTATTGTCG 251 GCGACGGTCA AACCGTTTGG CTCTACGATG TCGATTTGGC ACAAGTGACC 301 AAGTCGTCCC AAGACCAGGC CATAGGCGGC AGCCCCGCCG CCATCCTGTC 351 GAACAAAACC GCCCTCGAAA GCAGCTACAC GCTGAAAGAG GACGGTTCGT 401 CCAACGGCAT CGATTATGTG GGCAACGCCC AAACGCAACA ACGCCGGCTA 451 CCAATACATC CGCATCGGCT TCAAAGGCGG CAACCTCGCC GCCATGCAGC 501 TTAA This corresponds to the amino acid sequence <SEQ ID 1342; 502217.a>: a502.pep 1 MMKPHΝLFQF LAVCSLTVSV ASAQAGAVDA LKQFΝΝDADG ISGSFTQTVQ 51 SKKKTQTAHG TFKILRPGLF KWEYTSPYKQ TIVGDGQTVW LYDVDLAQVT 101 KSSQDQAIGG SPAAILSΝKT ALESSYTLKE DGSSΝGIDYV GΝAQTQQRRL 151 PIHPHRLQRR QPRRHAA* m502/a502 95.2% identity in 167 aa overlap 10 20 30 40 50 60 m502. pep MMKPHΝLFQFLAVCSLTVAVASAQAGAVDALKQFΝΝDADGISGSFTQXVQXKKKTQTAHG I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I lllllllll a502 MMKPHΝLFQFLAVCSLTVSVASAQAGAVDALKQFΝΝDADGISGSFTQTVQSKKKTQTAHG 10 20 30 40 50 60 70 80 90 100 110 120 m502. pep TFKILRPGLFKWEYTKLYRQTIVGDGQTVWLYDVDLAQVTKSSQDQAIGXSPAAILSΝKX lllllllllllllll: I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I lllllllll: a502 TFKILRPGLFKWEYTSPYKQTIVGDGQTVWLYDVDLAQVTKSSQDQAIGGSPAAILSΝKT 70 80 90 100 110 120 130 140 150 160 m502.pep ALESSYTLKEDGSSΝGIDYVGΝAQTQQRRLPIHPHRLQRRQPRRHAAX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a502 ALEΞSYTLKEDGSSΝGIDYVGΝAQTQQRRLPIHPHRLQRRQPRRHAAX 130 140 150 160 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1343>: g502-l.seq 1 ATGatGAAAc cgcaCaacct gttccaaTTc CTCGCCGTTT GCTCCCTGAC 51 CGTCGCCGTC GCTTCCGCAC AGGCGGGCGC GGTGGACGCG CTCAAGCAAT 101 TCAACAACGA TGCCGACGGT ATCAGCGGCA GCTTCACCCA AACCGTCCAA 151 AGCAAAAAGA AAACCCAAAC CGCGCACGGC ACGTTCAAAA TCCTGCGCCC 201 GGGCCTCTTC AAATGGGAAT ACACTTTGCC CTACAGACAG ACTATTGTCG 251 GCGACGGTCA AACCGTTTGG CTCTACGATG TTGATTTGGC ACAAGTGACC 301 AAGTCGTCCC AAGACCAGGC CATCGGCGGC AGCCCCGCCG CCATCCTGTC 351 GAACAAAACC GCCCTCGAAA GCAGTTACAC GCTGAAAGAG GACGGTTCGT 401 CCAACGGCAT CGATTATGTG CGGGCAACGC CCAAACGCAA CAACGCCGGC 451 TACCAATACA TCCGCATCGG CTTCAAAGGC GGCAACCTCG CCGCCATGCA 501 GCTTAAAGAC AGCTTCGGCA ACCAAACCTC CATCAGTTTC GGCGGTTTGA 551 ATACCAATCC CCAACTCTCG CGCGGCGCGT TCAAGTTTAC CCCGCCCAAA 601 GGCGTGGACG TGTTGAGCAA CTGA This corresponds to the amino acid sequence <SEQ ID 1344; ORF 502-l.ng>: g502-l.pep 1 MMKPHΝLFQF LAVCSLTVAV ASAQAGAVDA LKQFΝΝDADG ISGSFTQTVQ 51 SKKKTQTAHG TFKILRPGLF KWEYTLPYRQ TIVGDGQTVW LYDVDLAQVT 101 KSSQDQAIGG SPAAILSΝKT ALESSYTLKE DGSSΝGIDYV RATPKRΝΝAG 151 YQYIRIGFKG GΝLAAMQLKD SFGΝQTSISF GGLΝTΝPQLS RGAFKFTPPK 201 GVDVLSΝ* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1345>: a502-l.seq 1 ATGATGAAAC CGCACAACCT GTTCCAATTC CTCGCCGTTT GCTCCCTGAC 51 CGTCGCCGTC GCTTCCGCAC AGGCGGGCGC GGTAGACGCG CTTAAGCAAT 101 TCAACAACGA TGCCGACGGT ATCAGCGGCA GCTTCACCCA AACCGTCCAA 151 AGCAAAAAGA AAACCCAAAC CGCGCACGGC ACGTTCAAAA TCCTGCGACC 201 GGGCCTTTTC AAATGGGAAT ACACCAAACC TTACAGGCAA ACCATCGTCG 251 GCGACGGTCA AACCGTTTGG CTCTACGATG TTGATCTGGC ACAAGTGACC 301 AAGTCGTCCC AAGACCAGGC CATAGGCGGC AGCCCCGCCG CCATCCTGTC 351 GAACAAAACC GCCCTCGAAA GCAGCTACAC GCTGAAAGAG GACGGTTCGT 401 CCAACGGCAT CGATTATGTG CTGGCAACGC CCAAACGCAA CAACGCCGGC 451 TACCAATACA TCCGCATCGG CTTCAAAGGC GGCAACCTCG CCGCCATGCA 501 GCTTAAAGAC AGCTTCGGCA ACCAAACCTC CATCAGTTTC GGCGGTTTGA 551 ATACCAATCC CCAACTCTCG CGCGGCGCGT TCAAGTTTAC CCCGCCCAAA 601 GGCGTGGACG TGTTGAGCAA CTGA This corresponds to the amino acid sequence <SEQ ID 1346; ORF 502-l>: rα502-l.pep 1 MMKPHΝLFQF LAVCSLTVAV ASAQAGAVDA LKQFΝΝDADG ISGSFTQTVQ 51 SKKKTQTAHG TFKILRPGLF KWEYTKPYRQ TIVGDGQTVW LYDVDLAQVT 101 KSSQDQAIGG SPAAILSΝKT ALESSYTLKE DGSSΝGIDYV LATPKRΝΝAG 151 YQYIRIGFKG GΝLAAMQLKD SFGΝQTΞISF GGLΝTΝPQLS RGAFKFTPPK 201 GVDVLSΝ* m502-l/g502-l 99.0% identity in 207 aa overlap 10 20 30 40 50 60 m502-l.pep MMKPHΝLFQFLAVCSLTVAVASAQAGAVDALKQFΝΝDADGISGΞFTQTVQSKKKTQTAHG I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I II I I I I I I I I II I I I I I I I I I I I I I g502-l MMKPHΝLFQFLAVCSLTVAVASAQAGAVDALKQFΝΝDADGISGSFTQTVQΞKKKTQTAHG 10 20 30 40 50 60 70 80 90 100 110 120 m502-1. pep TFKILRPGLFKWEYTKPYRQTIVGDGQTVWLYDVDLAQVTKSSQDQAIGGSPAAILSΝKT I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I g502-l TFKILRPGLFKWEYTLPYRQTIVGDGQTVWLYDVDLAQVTKSSQDQAIGGSPAAILSΝKT 70 80 90 100 110 120 130 140 150 160 170 180 m502-l.pep ALESΞYTLKEDGSΞΝGIDYVLATPKRΝΝAGYQYIRIGFKGGΝLAAMQLKDSFGΝQTΞISF I I I II I I II I I I I I I I I I I I I I I I I II I I I I I I I I II I I I I I I I I I I I I I I I I I M I I I g502-l ALESSYTLKEDGSSΝGIDYVRATPKRΝΝAGYQYIRIGFKGGΝLAAMQLKDSFGΝQTSISF 130 140 150 160 170 180 190 200 m502-1. pep GGLNTNPQLSRGAFKFTPPKGVDVLSNX I I I I I I I I I I I I I I I I I I I I I I I I I I I I g502-l GGLNTNPQLSRGAFKFTPPKGVDVLSNX 190 200 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1347>: a502-l.seq 1 ATGATGAAAC CGCACAACCT GTTCCAATTC CTCGCCGTTT GCTCCCTGAC 51 CGTCTCCGTC GCTTCCGCAC AGGCGGGCGC GGTGGACGCG CTCAAGCAAT 101 TCAACAACGA TGCCGACGGT ATCAGCGGCA GCTTCACCCA AACCGTCCAA 151 AGCAAAAAGA AAACCCAAAC CGCGCACGGC ACGTTCAAAA TCCTGCGCCC 201 GGGCCTCTTT AAATGGGAAT ACACTTCGCC TTACAAACAG ACTATTGTCG 251 GCGACGGTCA AACCGTTTGG CTCTACGATG TCGATTTGGC ACAAGTGACC 301 AAGTCGTCCC AAGACCAGGC CATAGGCGGC AGCCCCGCCG CCATCCTGTC 351 GAACAAAACC GCCCTCGAAA GCAGCTACAC GCTGAAAGAG GACGGTTCGT 401 CCAACGGCAT CGATTATGTG CTGGCAACGC CCAAACGCAA CAACGCCGGC 451 TACCAATACA TCCGCATCGG CTTCAAAGGC GGCAACCTCG CCGCCATGCA 501 GCTTAAAGAC AGCTTCGGCA ATCAAACCTC CATCAGTTTC GGCGGTTTGA 551 ATACCAATCC CCAACTCTCG CGCGGCGCGT TCAAGTTTAC CCCGCCCAAA 601 GGCGTGGACG TGTTGAGCAA CTGA This corresponds to the amino acid sequence <SEQ ID 1348; ORF 502-1. a>: a502-l.pep 1 MMKPHΝLFQF LAVCSLTVSV ASAQAGAVDA LKQFΝΝDADG ISGSFTQTVQ 51 SKKKTQTAHG TFKILRPGLF KWEYTSPYKQ TIVGDGQTVW LYDVDLAQVT 101 KSSQDQAIGG SPAAILSΝKT ALESSYTLKE DGSSΝGIDYV LATPKRΝΝAG 151 YQYIRIGFKG GΝLAAMQLKD SFGΝQTSISF GGLΝTΝPQLΞ RGAFKFTPPK 201 GVDVLΞΝ* a502-l/m502-l 98.6% identity in 207 aa overlap 10 20 30 40 50 60 a502-l.pep MMKPHΝLFQFLAVCSLTVSVASAQAGAVDALKQFΝΝDADGISGSFTQTVQSKKKTQTAHG I I I I I I I I I I I I I I I I I h I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m502-l MMKPHΝLFQFLAVCSLTVAVASAQAGAVDALKQFΝΝDADGIΞGSFTQTVQSKKKTQTAHG 10 20 30 40 50 60 70 80 90 100 110 120 a502-l.pep TFKILRPGLFKWEYTSPYKQTIVGDGQTVWLYDVDLAQVTKSSQDQAIGGSPAAILSΝKT I I I I I I I I I I I I I I hi I :l I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m502-l TFKILRPGLFKWEYTKPYRQTIVGDGQTVWLYDVDLAQVTKSSQDQAIGGSPAAILSΝKT 70 80 90 100 110 120 130 140 150 160 170 180 a502-l.pep ALESSYTLKEDGSSΝGIDYVLATPKRΝΝAGYQYIRIGFKGGΝLAAMQLKDSFGΝQTSISF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m502-l ALESSYTLKEDGSSΝGIDYVLATPKRΝΝAGYQYIRIGFKGGΝLAAMQLKDΞFGΝQTΞISF 130 140 150 160 170 180 190 200 a502-l .pep GGLNTNPQLSRGAFKFTPPKGVDVLSNX I I I I I I I I I I I I I I I I I I I I I I I I I I I I m502-l GGLNTNPQLSRGAFKFTPPKGVDVLSNX 190 200 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1349>: g503. seq 1 atgtccgcgc cgtcggcatc ggtaatcatt ttgttccatg ccgcttcgat 51 ttcggcatcg agctgttcgg ggaagggcgt gtccaaaatc cattggcgga 101 tttctttgcc gacgcgtgcc agttcggaaa cgtcttcgac atccaatttt 151 gccagagcgg cggaaatgcg ttcgttcaga ccgttgtgtg cgagaaatgc 201 gcggtag This corresponds to the amino acid sequence <SEQ ID 1350; ORF 503.ng>: g503.pep 1 MSAPSASVII LFHAASISAS SCSGKGVSKI HWRISLPTRA SSETSSTSΝF 51 ARAAEMRSFR PLCARΝAR* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1351 >: m503. seq 1 atgtccgcac cgccggcatc ggcaaccatt ttgttccatg ccgcttcgat 51 ttcggcatcg agctgttcgg ggaaaggcgt atccaaaatc cattggcgga 101 tttctttgcc gacgcgtgcc agttcggcaa cgtcttcgac atccaatttt 151 gccagtgcgg cggaaatgcg ttcgctcaga ccgttgtgtg cgaggaatgc 201 gcggtag This corresponds to the amino acid sequence <SEQ ID 1352; ORF 503>: m503.pep 1 MSAPPASATI LFHAASISAS SCSGKGVSKI HWRISLPTRA SSATSSTSNF 51 ASAAEMRSLR PLCARNAR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 503 shows 91.2% identity over a 68 aa overlap with a predicted ORF (ORF 503.ng) from N gonorrhoeae: m503/g503 10 20 30 40 50 60 m503. pep MSAPPASATILFHAASISASSCSGKGVSKIHWRISLPTRASSATSSTSΝFASAAEMRSLR llll II: I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I llllllll I I I I I I : I g503 MSAPSASVIILFHAASISASSCSGKGVSKIHWRISLPTRASSETSSTSΝFARAAEMRSFR 10 20 30 40 50 60 69 m503.pep PLCARΝAR g503 PLCARΝAR The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1353>: a503. seq 1 ATGTCCGCGC CGCCGGCATC GGCAACCATT TTGTTCCATG CCGCTTCGAT 51 TTCGGCATCG AGCTGTTCGG GGAAGGGCGT GTCCAAAATC CATTGGCGGA 101 TTTCTTTGCC GACGCGTGCC AGTTCGGCAA CGTCTTCGAC ATCTAATTTT 151 GCCAGTGCGG CGGAAATGCG TTCGCTCAGA CCGTTGTGTG CGAGGAATGC 201 GCGGTAG This corresponds to the amino acid sequence <SEQ ID 1354; ORF 503. a>: a503.pep 1 MSAPPASATI LFHAASISAS SCSGKGVSKI HWRISLPTRA SSATSSTSNF 51 ASAAEMRSLR PLCARNAR* m503/a503 100.0% identity in 68 aa overlap 10 20 30 40 50 60 m503.pep MSAPPASATILFHAASISASSCSGKGVSKIHWRISLPTRASSATSSTSNFASAAEMRSLR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a503 MSAPPASATILFHAASISASSCSGKGVSKIHWRISLPTRASSATSSTSNFASAAEMRSLR 10 20 30 40 50 60 69 m503.pep PLCARNARX a503 PLCARNARX The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1355>: g503-l.seq 1 ATGGCGCGGT CGTTGTACAG GGAGGCGAAA ACGTGGCGCA TCGCTTTTTT 51 AACGTTATCC AAGCCATTGA TATTCAGGAA GGTTTCCTGT TGGCCGGCAA 101 ATGATGCGTC GGGCAGGTCT TCGGCGGTTG CGGAAGAGCG TACGGCAACG 151 GAAATGTCCG CGCCGTCGGC ATCGGTAATC ATTTTGTTCC ATGCCGCTTC 201 GATTTCGGCA TCGAGCTGTT CGGGGAAGGG CGTGTCCAAA ATCCATTGGC 251 GGATTTCTTT GCCGACGCGT GCCAGTTCGG AAACGTCTTC GACATCCAAT 301 TTTGCCAGAG CGGCGGAAAT GCGTTCGTTC AGACCGTTGT GTGCGAGAAA 351 TGCGCGGTAG This corresponds to the amino acid sequence <SEQ ID 1356; ORF 214.ng>: g503-l.pep 1 MARSLYREAK TWRIAFLTLS KPLIFRKVSC WPANDASGRS SAVAEERTAT 51 EMSAPSASVI ILFHAASISA SSCSGKGVSK IHWRISLPTR ASSETΞSTSN 101 FARAAEMRSF RPLCARNAR* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1357>: m503-l.seq 1 ATGGCACGGT CGTTATACAG GGAAGCGAAT ACATGGTGCA TCGCTTCTTT 51 AACGTTATCC AAGCCGTTGA TGTTCAAGAA GGTTTCCTGT TGTCCAGCGA 101 ATGATGCGTC CGGCAGGTCT TCGGCAGTTG CGGAAGAACG TACGGCAACG 151 GAAATGTCCG CACCGCCGGC ATCGGCAACC ATTTTGTTCC ATGCCGCTTC 201 GATTTCGGCA TCGAGCTGTT CGGGGAAAGG CGTATCCAAA ATCCATTGGC 251 GGATTTCTTT GCCGACGCGT GCCAGTTCGG CAACGTCTTC GACATCCAAT 301 TTTGCCAGTG CGGCGGAAAT GCGTTCGCTC AGACCGTTGT GTGCGAGGAA 351 TGCGCGGTAG This corresponds to the amino acid sequence <SEQ ID 1358; ORF 503- 1>: m503-l.pep 1 MARSLYREAΝ TWCIASLTLS KPLMFKKVSC CPAΝDASGRΞ SAVAEERTAT 51 EMSAPPASAT ILFHAASISA SSCSGKGVSK IHWRISLPTR ASSATΞSTSΝ 101 FASAAEMRSL RPLCAR AR* g503-l / m503-l 89.9% identity in 119 aa overlap 10 20 30 40 50 60 g503-l.pep MARSLYREAKTWRIAFLTLSKPLIFRKVSCWPANDASGRSΞAVAEERTATEMSAPSAΞVI I I I I I I I I hi I II I I I I I I I : I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I Ih m503-l MARSLYREANTWCIASLTLSKPLMFKKVSCCPANDASGRSSAVAEERTATEMSAPPASAT 10 20 30 40 50 60 70 80 90 100 110 120 g503-l.pep ILFHAASIΞASSCSGKGVSKIHWRIΞLPTRASSETSSTSNFARAAEMRSFRPLCARNARX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I l l I I I I I I : I I I I I I I I I I m503-l ILFHAASISASSCSGKGVSKIHWRISLPTRASSATSSTSNFAΞAAEMRSLRPLCARNARX 70 80 90 100 110 120 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1359>: a503-l.seq 1 ATGGCGCGGT CGTTGTACAG GGAGGCGAAT ACATGGCGCA TCGCTTCTTT 51 AACGTTTTCC AAGCCGTTGA TATTCAGGAA GGTTTCCTGT TGGCCGGCAA 101 ATGATGCGTC GGGCAGGTCT TCGGCGGTTG CGGAAGAGCG TACGGCAACG 151 GAAATGTCCG CGCCGCCGGC ATCGGCAACC ATTTTGTTCC ATGCCGCTTC 201 GATTTCGGCA TCGAGCTGTT CGGGGAAGGG CGTGTCCAAA ATCCATTGGC 251 GGATTTCTTT GCCGACGCGT GCCAGTTCGG CAACGTCTTC GACATCTAAT 301 TTTGCCAGTG CGGCGGAAAT GCGTTCGCTC AGACCGTTGT GTGCGAGGAA 351 TGCGCGGTAG This corresponds to the amino acid sequence <SEQ ID 1360; ORF 503-l.a>: a503-l.pep 1 MARSLYREAΝ TWRIASLTFS KPLIFRKVSC WPANDASGRS SAVAEERTAT 51 EMSAPPASAT ILFHAASISA SSCSGKGVSK IHWRISLPTR ASSATSSTSN 101 FASAAEMRSL RPLCARNAR* a503-l / m503-l 95.8% identity in 119 aa overlap 10 20 30 40 50 60 a503-l.pep MARΞLYREANTWRIASLTFSKPLIFRKVSCWPANDASGRSSAVAEERTATEMSAPPASAT I I I II I I I I I I I I I I I I: I I I I : I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m503-l MARSLYREANTWCIASLTLSKPLMFKKVSCCPANDASGRSSAVAEERTATEMSAPPASAT 10 20 30 40 50 60 70 80 90 100 110 120 a503-l.pep I FHAASISASSCSGKGVSKIHWRISLPTRASSATSSTSNFASAAEMRSLRPLCARNARX II I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m503-l ILFHAASISAΞSCSGKGVSKIHWRISLPTRASSATSΞTΞNFASAAEMRSLRPLCARNARX 70 80 90 100 110 120 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1361>: g504. seq 1 atgttggttc aggacttgcc ttttgaagtc aaactgaaaa aattccatat 51 cgatttttac aatacgggta tgccgcgcga ttttgccagc gatattgaag 101 taacggacaa ggcaaccggt gagaaactcg agcgcaccat ccgcgtgaac 151 catcctttga ccttgcacgg catcacgatt tatcaggcga gttttgccga 201 cggcggttcg gatttgacat tcaaggcgtg gaatttgagg gatgcttcgc 251 gcgaacctgt cgtgttgaag gcaacctcca tacaccagtt tccgttggaa 301 atcggcaaac acaaatatcg tcttgagttc gatcagttca cttctatgaa 351 tgtggaggac atgagcgagg gtgcggaacg ggaaaaaagc ctgaaatcca 401 ctctgaacga tgtccgcgcc gttactcagg aaggtaaaaa atacaccaat 451 atcggccctt ccatcgtgta ccgcatccgt gatgcggcag ggcaggcggt 501 cgaatataaa aactatatgc tgccgatttt gcaggacaaa gattattttt 551 ggctgaccgg cacgcgcagc ggcttgcagc agcaataccg ctggctgcgt 601 atccccttgg acaagcagtt gaaagcggac acctttatgg cattgcgtga 651 gtttttgaaa gatggggaag ggcgcaaacg tctggttgcc gacgcaacca 701 aagacgcacc tgccgaaatc cgcgaacaat tcatgctggc tgcggaaaac 751 acgctgaata tctttgcgca aaaaggctat ttgggattgg acgaatttat 801 tacgtccaat atcccgaaag ggcagcagga taagatgcag ggctatttct 851 acgaaatgct ttacggcgtg atgaacgctg ctttggatga aaccatacgc 901 cggtacggct tgcccgaatg gcagcaggat gaagcgcgga accgtttcct 951 gctgcacagt atggatgcct atacggggct gacggaatat cccgcgccta 1001 tgctgctcca gcttgacggg ttttccgagg tgcgttcctc aggtttgcag 1051 atgacccgtt cgccgggtgc gcttttggtc tatctcggct cggtattgtt 1101 ggttttgggt acagtattta tgttttatgt gcccaaaaaa cgggcgtggg 1151 tattgttttc aaacdgcaaa atccgttttg ctatgtcttc ggcccgcagc 1201 gaacgggatt tgcagaagga atttccaaaa cacgtcgaga gcctgcaacg 1251 gctcggcaag gacttgaatc atgactga This corresponds to the amino acid sequence <SEQ ID 1362; ORF 504.ng>: g504.pep 1 MLVQDLPFEV KLKKFHIDFY ΝTGMPRDFAS DIEVTDKATG EKLERTIRVΝ 51 HPLTLHGITI YQASFADGGS DLTFKAWΝLR DASREPWLK ATSIHQFPLE 101 IGKHKYRLEF DQFTSMΝVED MSEGAEREKS LKSTLΝDVRA VTQEGKKYTΝ 151 IGPSIVYRIR DAAGQAVEYK ΝYMLPILQDK DYFWLTGTRS GLQQQYRWLR 201 IPLDKQLKAD TFMALREFLK DGEGRKRLVA DATKDAPAEI REQFMLAAEΝ 251 TLΝIFAQKGY LGLDEFITSΝ IPKGQQDKMQ GYFYEMLYGV MΝAALDETIR 301 RYGLPEWQQD EARΝRFLLHS MDAYTGLTEY PAPMLLQLDG FSEVRSSGLQ 351 MTRSPGALLV YLGSVLLVLG TVFMFYVPKK RAWVLFSΝKI RFAMSSARSE 401 RDLQKEFPKH VESLQRLGKD LΝHD* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1363>: m504. seq.. 1 atattggttc aggacttgcc ttttgaagtc aaactgaaaa aattccatat 51 cgatttttac aatacgggta tgccgcgtga tttcgccagc gatattgaag 101 tgacggacaa ggcaaccggt gagaaactcg agcgcaccat ccgcgtgaac 151 catcctttga ccttgcacgg catcacgatt tatcaggcga gttttgccga 201 cggcggttcg gatttgacat tcaaggcgtg gaatttgggt gatgcttcgc 251 gcgagcctgt cgtgttgaag gcaacatcca tacaccagtt tccgttggaa 301 attggcaaac acaaatatcg tcttgagttc gatcagttca cttctatgaa 351 tgtggaggac atgagcgagg gcgcggaacg ggaaaaaagc ctgaaatcca 401 cgctgmmcga tgtccgcgcc gttactcagg aaggtaaaaa atacaccaat 451 atcggccctt ccattgttta ccgtatccgt gatgcggcag ggcaggcggt 501 cgaatataaa aactatatgc tgccggtttt gcaggaacag gattattttt 551 ggattaccgg cacgcgcagc ggcttgcagc agcaataccg ctggctgcgt 601 atccccttgg acaagcagtt gaaagcggac acctttatgg cattgcgtga 651 gtttttgaaa gatggggaag ggcgcaaacg tctggttgcc gacgcaacca 701 aaggcgcacc tgccgaaatc cgcgaacaat tcatgctggc tgcggaaaac 751 acgctgaaca tctttgcaca aaaaggctat ttgggattgg acgaatttat 801 tacgtccaat atcccgaaag agcagcagga taagatgcag ggctatttct 851 acgaaatgct ttacggcgtg atgaacgctg ctttggatga aaccatacgc 901 cggtacggct tgcccgaatg gcagcaggat gaagcgcgga atcgtttcct 951 gctgcacagt atggatgcgt acacgggttt gaccgaatat cccgcgccta 1001 tgctgctgca acttgatggg ttttccgagg tgcgttcgtc gggtttgcag 1051 atgacccgtt ccccgggtgc gcttttggtc tatctcggct cggtgctgtt 1101 ggtattgggt acggtattga tgttttatgt gcgcgaaaaa cgggcgtggg 1151 tattgttttc agacggcaaa atccgttttg ccatgtcttc ggcccgcagc 1201 gaacgggatt tgcagaagga atttccaaaa cacgtcgaga gtctgcaacg 1251 gctcggcaag gacttgaatc atga This corresponds to the amino acid sequence <SEQ ID 1364; ORF 504>: m504.pep.. 1 ILVQDLPFEV KLKKFHIDFY NTGMPRDFAS DIEVTDKATG EKLERTIRVN 51 HPLTLHGITI YQASFADGGS DLTFKAWNLG DASREPWLK ATSIHQFPLE 101 IGKHKYRLEF DQFTSMNVED MSEGAEREKS LKSTLXDVRA VTQEGKKYTN 151 IGPSIVYRIR DAAGQAVEYK NYMLPVLQEQ DYFWITGTRS GLQQQYRWLR 201 IPLDKQLKAD TFMALREFLK DGEGRKRLVA DATKGAPAEI REQFMLAAEN 251 TLNIFAQKGY LGLDEFITSN IPKEQQDKMQ GYFYEMLYGV MNAALDETIR 301 RYGLPEWQQD EARNRFLLHS MDAYTGLTEY PAPMLLQLDG FSEVRSSGLQ 351 MTRSPGALLV YLGSVLLVLG TVLMFYVREK RAWVLFSDGK IRFAMSSARS 401 ERDLQKEFPK HVESLQRLGK DLNHD* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 504 shows 96.7% identity over a 425 aa overlap with a predicted ORF (ORF 504.ng) from N. gonorrhoeae: m504/g504 10 20 30 40 50 60 m504.pep ILVQDLPFEVKLKKFHIDFYΝTGMPRDFASDIEVTDKATGEKLERTIRVΝHPLTLHGITI MMMMMMMMMMIMMMMMMMIMMMMMMMMMMMM g504 MLVQDLPFEVKLKKFHIDFYΝTGMPRDFASDIEVTDKATGEKLERTIRVΝHPLTLHGITI 10 20 30 40 50 60 70 80 90 100 110 120 m504.pep YQASFADGGSDLTFKAWΝLGDASREPWLKATSIHQFPLEIGKHKYRLEFDQFTSMΝVED MMMMMIMMMM M II I II II M 111 II II II II II II II I II M II II I II g504 YQASFADGGSDLTFKAWΝLRDASREPWLKATSIHQFPLEIGKHKYRLEFDQFTSMΝVED 70 80 90 100 110 120 130 140 150 160 170 180 m504. pep MSEGAEREKSLKSTLXDVRAVTQEGKKYTΝIGPSIVYRIRDAAGQAVEYKΝYMLPVLQEQ MMMMMMMMMMMMMMMIMMMMMh: g504 MSEGAEREKSLKSTLΝDVRAVTQEGKKYTΝIGPSIVYRIRDAAGQAVEYKΝYMLPILQDK 130 140 150 160 170 180 190 200 210 220 230 240 m504.pep DYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGRKRLVADATKGAPAEI MMMMMMMMMMMMMMMMMMMMMMMMMMM Mill g504 DYFWLTGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGRKRLVADATKDAPAEI 190 200 210 220 230 240 250 260 270 280 290 300 m504.pep REQFMLAAEΝTLΝIFAQKGYLGLDEFITSΝIPKEQQDKMQGYFYEMLYGVMΝAALDETIR IMMMMMMMMIMMMIMMMM 111 II II I II I II 1111 II II I II II g504 REQFMLAAEΝTLΝIFAQKGYLGLDEFITSΝIPKGQQDKMQGYFYEMLYGVMΝAALDETIR 250 260 270 280 290 300 310 320 330 340 350 360 m504. pep RYGLPEWQQDEARΝRFLLHSMDAYTGLTEYPAPMLLQLDGFSEVRSSGLQMTRSPGALLV 1 ! I E E E ! 1111111111 i 11 E j 1 i ! E 11 j ! I j 11 F F ! I r ! 11 E E 11 r 11111111 F 11 f I g504 RYGLPEW QQ DEARΝRFLLHSMDAYTGLTEYPAPMLLQLDGFSEVRSSGLQMTRSPGALLV 310 320 330 340 350 360 370 380 390 400 410 420 m504.pep YLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSARSERDLQKEFPKHVESLQRLGK g504 YLGSVLLVLGTVFMFYVPKKRAWVLFSN-KIRFAMSSARSERDLQKEFPKHVESLQRLGK 370 380 390 400 410 m504.pep DLNHD Mill g504 DLNHD 420 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 1365>: a504. seq 1 ATATTGGTTC AGGACTTGCC TTTTGAAGTC AAACTGAAAA AATTCCATAT 51 CGATTTTTAC AATACGGGTA TGCCGCGCGA TTTTGCCAGT GATATTGAAG 101 TAACGGATAA GGCAACCGGT GAGAAACTCG AGCGCACCAT CCGCGTGAAC 151 CATCCTTTGA CCTTGCACGG CATCACGATT TATCAGGCGA GTTTTGCCGA 201 CGGCGGTTCG GATTTGACAT TCAAGGCGTG GAATTTGGGT GATGCTTCGC 251 GCGAGCCTGT CGTGTTGAAG GCAACATCCA TACACCAGTT TCCGTTGGAA 301 ATTGGCAAAC ACAAATATCG TCTTGAGTTC GATCAGTTTA CTTCTATGAA 351 TGTGGAGGAC ATGAGCGAGG GCGCGGAACG GGAAAAAAGC CTGAAATCCA 401 CGCTGAACGA TGTCCGCGCC GTTACTCAGG AAGGTAAAAA ATACACCAAT 451 ATCGGCCCTT CCATTGTTTA CCGTATCCGT GATGCGGCAG GGCAGGCGGT 501 CGAATATAAA AACTATATGC TGCCGGTTTT GCAGGAACAG GATTATTTTT 551 GGATTACCGG CACGCGCAGC GGCTTGCAGC AGCAATACCG CTGGCTGCGT 601 ATCCCCTTGG ACAAGCAGTT GAAAGCGGAC ACCTTTATGG CATTGCGTGA 651 GTTTTTGAAA GATGGGGAAG GGCGCAAACG TCTGGTTGCC GACGCAACCA 701 AAGGCGCACC TGCCGAAATC CGCGAACAAT TCATGCTGGC TGCGGAAAAC 751 ACGCTGAACA TCTTTGCACA AAAAGGCTAT TTGGGATTGG ACGAATTTAT 801 TACGTCCAAT ATCCCGAAAG AGCAGCAGGA TAAGATGCAG GGCTATTTCT 851 ACGAAATGCT TTACGGCGTG ATGAACGCTG CTTTGGATGA AACCATACGC 901 CGGTACGGCT TGCCCGAATG GCAGCAGGAT GAAGCGCGGA ATCGTTTCCT 951 GCTGCACAGT ATGGATGCGT ACACGGGTTT GACCGAATAT CCCGCGCCTA 1001 TGCTGCTGCA ACTTGATGGG TTTTCCGAGG TGCGTTCGTC GGGTTTGCAG 1051 ATGACCCGTT CCCCGGGTGC GCTTTTGGTC TATCTCGGCT CGGTGCTGTT 1101 GGTATTGGGT ACGGTATTGA TGTTTTATGT GCGCGAAAAA CGGGCGTGGG 1151 TATTGTTTTC AGACGGCAAA ATCCGTTTTG CCATGTCTTC GGCCCGCAGC 1201 GAACGGGATT TGCAGAAGGA ATTTCCAAAA CACGTCGAGA GTCTGCAACG 1251 GCTCGGCAAG GACTTGAATC ATGACTGA This corresponds to the amino acid sequence <SEQ ID 1366; ORF 504.a>: a504.pep 1 ILVQDLPFEV KLKKFHIDFY ΝTGMPRDFAS DIEVTDKATG EKLERTIRVΝ 51 HPLTLHGITI YQASFADGGS DLTFKAWNLG DASREPWLK ATSIHQFPLE 101 IGKHKYRLEF DQFTSMNVED MSEGAEREKS LKSTLNDVRA VTQEGKKYTN 151 IGPSIVYRIR DAAGQAVEYK NYMLPVLQEQ DYFWITGTRS GLQQQYRWLR 201 IPLDKQLKAD TFMALREFLK DGEGRKRLVA DATKGAPAEI REQFMLAAEN 251 TLNIFAQKGY LGLDEFITSN IPKEQQDKMQ GYFYEMLYGV MNAALDETIR 301 RYGLPEWQQD EARNRFLLHS MDAYTGLTEY PAPMLLQLDG FSEVRSSGLQ 351 MTRSPGALLV YLGSVLLVLG TVLMFYVREK RAWVLFSDGK IRFAMSSARS 401 ERDLQKEFPK HVESLQRLGK DLNHD* m504/a504 99.8% identity in 425 aa overlap 10 20 30 40 50 60 m504.pep ILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNHPLTLHGITI I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a504 ILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNHPLTLHGITI 10 20 30 40 50 60 70 80 90 100 110 120 m504. pep YQASFADGGSDLTFKAWNLGDASREPWLKATSIHQFPLEIGKHKYRLEFDQFTSMNVED I I I I I I I M I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a504 YQASFADGGSDLTFKAWNLGDASREPWLKATSIHQFPLEIGKHKYRLEFDQFTSMNVED 70 80 90 100 110 120 130 140 150 160 170 180 m50 . pep MSEGAEREKSLKSTLXDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAVEYKNYMLPVLQEQ lllllllllllllll I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a504 MSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAVEYKNYMLPVLQEQ 130 140 150 160 170 180 190 200 210 220 230 240 m504. pep DYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGRKRLVADATKGAPAEI I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a504 DYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGRKRLVADATKGAPAEI 190 200 210 220 230 240 250 260 270 280 290 300 m504. pep REQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYFYEMLYGVMNAALDETIR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a504 REQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYFYEMLYGVMNAALDETIR 250 260 270 280 290 300 310 320 330 340 350 360 m504. pep RYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGFSEVRSSGLQMTRSPGALLV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a504 RYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGFSEVRSSGLQMTRSPGALLV 310 320 330 340 350 360 370 380 390 400 410 420 m504. pep YLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSARSERDLQKEFPKHVESLQRLGK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I a504 YLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSARSERDLQKEFPKHVESLQRLGK 370 380 390 400 410 420 m504.pep DLNHDX llllll a504 DLNHDX The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1367>: g505. seq 1 atgtttcgtt tacaattcag gctgtttccc cctttgcgaa ccgccatgca 51 catcctgttg accgccctgc tcaaatgcct ctccctgctg tcgctttcct 101 gtctgcacac gctgggaaac cggctcggac atctggcgtt ttacctttta 151 aaggaagacc gcgcgcgcat cgtcgccaat atgcggcagg cgggtttgaa 201 ccccgacacg cagacggtca aagccgtttt tgcggaaacg gcaaaatgcg 251 gtttggaact tgcccccgcg tttttcaaaa aaccggaaga catcgaaaca 301 atgttcaaag cggtacacgg ctgggaacac gtgcagcagg ctttggacaa 351 gggcgaaggg ctgctgttca tcacgccgca catcggcagc tacgatttgg 401 gcggacgcta catcagccag cagcttccgt tccacctgac cgccatgtac 451 aagccgccga aaatcaaagc gatagacaaa atcatgcagg cgggcagggt 501 gcgcggcaaa ggcaaaaccg cgcccaccgg catacaaggg gtcaaacaaa 551 tcatcaaggc cctgcgcgcg ggcgaggcaa ccatcatcct gcccgaccac 601 gtcccttctc cgcaggaagg cggcggcgtg tgggcggatt ttttcggcaa 651 acctgcatac accatgacac tggcggcaaa attggcacac gtcaaaggcg 701 tgaaaaccct gtttttctgc tgcgaacgcc tgcccgacgg acaaggcttc 751 gtgttgcaca tccgccccgt ccaaggggaa ttgaacggca acaaagccca 801 cgatgccgcc gtgttcaacc gcaataccga atattggata cgccgttttc 851 cgacgcagta tctgtttatg tacaaccgct ataaaacgcc gtaa This corresponds to the amino acid sequence <SEQ ID 1368; ORF 505.ng>: g505.pep 1 MFRLQFRLFP PLRTAMHILL TALLKCLSLL SLSCLHTLGΝ RLGHLAFYLL 51 KEDRARIVAΝ MRQAGLΝPDT QTVKAVFAET AKCGLELAPA FFKKPEDIET 101 MFKAVHGWEH VQQALDKGEG LLFITPHIGS YDLGGRYISQ QLPFHLTAMY 151 KPPKIKAIDK IMQAGRVRGK GKTAPTGIQG VKQIIKALRA GEATIILPDH 201 VPSPQEGGGV WADFFGKPAY TMTLAAKLAH VKGVKTLFFC CERLPDGQGF 251 VLHIRPVQGE LΝGΝKAHDAA VFΝRΝTEYWI RRFPTQYLFM YΝRYKTP* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1369>: m505 . seq (partial ) 1 GGCATGTTTC GTTTACAATT CAGGCTGTTT CCCCCTTTGC GAACCGCCAT 51 GCACATCCTG TTGACCGCCC TGCTCAAATG CCTCTCCCTG CTGCCGCTTT 101 CCTGTCTGCA CACGCTGGGA AACCGGCTCG GACATCTGGC GTTTTACCTT 151 TTAAAGGAAG ACCGCGCGCG CATCGTCGCC AATATGCGGC AGGCGGGTTT 201 GAACCCCGAC CCCAAAACGG TCAAAGCCGT TTTTGCGGAA ACGGCAAAAG 251 GCGGTTTGGA ACTTGCCCCC GCGTTTTTCA GAAAACCGGA AGACATAGAA 301 ACAATGTTCA AAGCGGTACA CGGCTGGGAA CATGTGCAGC AGGCTTTGGA 351 CAAACACGAA GGGCTGCTAT TCATCACGCC GCACATCGGC AGCTACGATT 401 TGGGCGGACG CTACATCAGC CAGCAGCTTC CGTTCCCGCT GACCGCCATG 451 TACAAACCGC CGAAAATCAA AGCGATAGAC AAAATCATGC AGGCGGGCAG 501 GGTTCGCGGC AAAGGAAAAA CCGCGCCTAC CAGCATACAA GGGGTCAAAC 551 AAATCATCAA AGCCCTGCGT TCGGGCGAgC AACCATCGTC CTGCCCGACC 601 ACGTCCCCTC CCCTCAAGAA GGCGGGGAAG GCGTATGGGT GGATTTCTTC 651 GGCAAACCTG CCTATACCAT GACGCTGGCG GCAArATTGG CACACGTCAA 701 AGGCGTGAAA ACCCTGTTTT TCTGCTGCGA ACGCCTGCCT GGCGGACAAG 751 GTTTCGATTT GCACATCCGC CCCGTCCAAG GGGAATTGAA CGGCGACAAA 801 GCCCATGATG CCGCCGTGTT CAACCGCAAT GCCGAATATT GGATACGCCG 851 TTTTCCGACG CAtATC .... This corresponds to the amino acid sequence <SEQ ID 1370; ORF 505>: m505.pep (partial) 1 MFRLQFRLFP PLRTAMHILL TALLKCLSLL PLSCLHTLGN RLGHLAFYLL 51 KEDRARIVAN MRQAGLNPDP KTVKAVFAET AKGGLELAPA FFRKPEDIET 101 MFKAVHGWEH VQQALDKHEG LLFITPHIGS YDLGGRYISQ QLPFPLTAMY 151 KPPKIKAIDK IMQAGRVRGK GKTAPTSIQG VKQIIKALRS GEATIVLPDH 201 VPSPQEGGEG VWVDFFGKPA YTMTLAAXLA HVKGVKTLFF CCERLPGGQG 251 FDLHIRPVQG ELNGDKAHDA AVFNRNAEYW IRRFPTHI ... Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 505 shows 93.7% identity over a 287 aa overlap with a predicted ORF (ORF 505.ng) from N. gonorrhoeae: m505/g505 10 20 30 40 50 60 m505.pep MFRLQFRLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGΝRLGHLAFYLLKEDRARIVAΝ MMMMMMMMMMMMMMM MMMMMMMMMMMIMMM g505 MFRLQFRLFPPLRTAMHILLTALLKCLSLLSLSCLHTLGΝRLGHLAFYLLKEDRARIVAΝ 10 20 30 40 50 60 70 80 90 100 110 120 m505.pep MRQAGLΝPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMFKAVHGWEHVQQALDKHEG IMIMIII :! 1111111111 II II I II I h II II I II II II II II II I II I II I II g505 MR Q AGLΝPDT Q TVKAVFAETAKCGLELAPAFFKKPEDIETMFKAVHGWEHVQQALDKGEG 70 80 90 100 110 120 130 140 150 160 170 180 m505.pep LLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDKIMQAGRVRGKGKTAPTSIQG M M M M M M M M M M M M I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M I I g505 LLFITPHIGSYDLGGRYISQQLPFHLTAMYKPPKIKAIDKIMQAGRVRGKGKTAPTGIQG 130 140 150 160 170 180 190 200 210 220 230 240 m505. pep VKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGKPAYTMTLAAXLAHVKGVKTLFF I I I I I I I I I I I II I : I I I I I I I I I I I I I I I: I I I I I I I I I I I I I I I I I I I I I I I I I I g505 VK Q IIKALRAGEATIILPDHVPSPQEGG-GVWADFFGKPAYTMTLAAKLAHVKGVKTLFF 190 200 210 220 230 250 260 270 280 289 m505. pep CCERLPGGQGFDLHIRPVQGELΝGDKAHDAAVFΝRΝAEYWIRRFPTHI llllll llll I I I I I I II I I I I : I I I I I I I I I I I : I I I I I I I I I : g505 CCERLPDG Q GFVLHIRPVQGELΝGΝKAHDAAVFΝRΝTEYWIRRFPTQYLFMYΝRYKTP 240 250 260 270 280 290 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1371>: a505. seq 1 ATGTTTCGTT TACAATTCAG GCTGTTTCCC CCTTTGCGAA CCGCCATGCA 51 CATCCTGTTG ACCGCCCTGC TCAAATGCCT CTCCCTGCTG CCGCTTTCCT 101 GTCTGCACAC GCTGGGAAAC CGGCTCGGAC ATCTGGCGTT TTACCTTTTA 151 AAGGAAGACC GCGCGCGCAT CGTCGCCAAT ATGCGTCAGG CAGGCATGAA 201 TCCCGACCCC AAAACGGTCA AAGCCGTTTT TGCGGAAACG GCAAAAGGCG 251 GTTTGGAACT TGCCCCCGCG TTTTTCAGAA AACCGGAAGA CATAGAAACA 301 ATGTTCAAAG CGGTACACGG CTGGGAACAT GTGCAGCAGG CTTTGGACAA 351 ACACGAAGGG CTGCTATTCA TCACGCCGCA CATCGGCAGC TACGATTTGG 401 GCGGACGCTA CATCAGCCAG CAGCTTCCGT TCCCGCTGAC CGCCATGTAC 451 AAACCGCCGA AAATCAAAGC GATAGACAAA ATCATGCAGG CGGGCAGGGT 501 TCGCGGCAAA GGAAAAACCG CGCCTACCAG CATACAAGGG GTCAAACAAA 551 TCATCAAAGC CCTGCGTTCG GGCGAAGCAA CCATCGTCCT GCCCGACCAC 601 GTCCCCTCCC CTCAAGAAGG CGGGGAAGGC GTATGGGTGG ATTTCTTCGG 651 CAAACCTGCC TATACCATGA CGCTGGCGGC AAAATTGGCA CACGTCAAAG 701 GCGTGAAAAC CCTGTTTTTC TGCTGCGAAC GCCTGCCTGG CGGACAAGGT 751 TTCGATTTGC ACATCCGCCC CGTCCAAGGG GAATTGAACG GCGACAAAGC 801 CCATGATGCC GCCGTGTTCA ACCGCAATGC CGAATATTGG ATACGCCGTT 851 TTCCGACGCA GTATCTGTTT ATGTACAACC GCTACAAAAT GCCGTAA This corresponds to the amino acid sequence <SEQ ID 1372; ORF 505. a>: a505.pep 1 MFRLQFRLFP PLRTAMHILL TALLKCLSLL PLSCLHTLGN RLGHLAFYLL 51 KEDRARIVAN MRQAGMNPDP KTVKAVFAET AKGGLELAPA FFRKPEDIET 101 MFKAVHGWEH VQQALDKHEG LLFITPHIGS YDLGGRYISQ QLPFPLTAMY 151 KPPKIKAIDK IMQAGRVRGK GKTAPTSIQG VKQIIKALRS GEATIVLPDH 201 VPSPQEGGEG VWVDFFGKPA YTMTLAAKLA HVKGVKTLFF CCERLPGGQG 251 FDLHIRPVQG ELNGDKAHDA AVFNRNAEYW IRRFPTQYLF MYNRYKMP* m505/a505 99.0% identity in 287 aa overlap 10 20 30 40 50 60 m505.pep MFRLQFRLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGHLAFYLLKEDRARIVAN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a505 MFRLQFRLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGHLAFYLLKEDRARIVAN 10 20 30 40 50 60 70 80 90 100 110 120 m505. .pep MRQAGLNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMFKAVHGWEHVQQALDKHEG I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a505 MRQAGMNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMFKAVHGWEHVQQALDKHEG 70 80 90 100 110 120 130 140 150 160 170 180 m505. •pep LLFITPHIGΞYDLGGRYISQQLPFPLTAMYKPPKIKAIDKIMQAGRVRGKGKTAPTSIQG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a505 LLFITPHIGSYDLGGRYIΞQQLPFPLTAMYKPPKIKAIDKIMQAGRVRGKGKTAPTSIQG 130 140 150 160 170 180 190 200 210 220 230 240 m505, .pep VKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGKPAYTMTLAAXLAHVKGVKTLFF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a505 VKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGKPAYTMTLAAKLAHVKGVKTLFF 190 200 210 220 230 240 250 260 270 280 m505, .pep CCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRNAEYWIRRFPTHI I I I I I I II I I I I I I I I I I I I I I I I I I I II I I I I I I I II I I I I I I I h a505 CCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRNAEYWIRRFPTQYLFMYNRYKMPX 250 260 270 280 290 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 1373>: m505-l . seq 1 ATGTTTCGTT TACAATTCAG GCTGTTTCCC CCTTTGCGAA CCGCCATGCA 51 CATCCTGTTG ACCGCCCTGC TCAAATGCCT CTCCCTGCTG CCGCTTTCCT 101 GTCTGCACAC GCTGGGAAAC CGGCTCGGAC ATCTGGCGTT TTACCTTTTA 151 AAGGAAGACC GCGCGCGCAT CGTCGCCAAT ATGCGGCAGG CGGGTTTGAA 201 CCCCGACCCC AAAACGGTCA AAGCCGTTTT TGCGGAAACG GCAAAAGGCG 251 GTTTGGAACT TGCCCCCGCG TTTTTCAGAA AACCGGAAGA CATAGAAACA 301 ATGTTCAAAG CGGTACACGG CTGGGAACAT GTGCAGCAGG CTTTGGACAA 351 ACACGAAGGG CTGCTATTCA TCACGCCGCA CATCGGCAGC TACGATTTGG 401 GCGGACGCTA CATCAGCCAG CAGCTTCCGT TCCCGCTGAC CGCCATGTAC 451 AAACCGCCGA AAATCAAAGC GATAGACAAA ATCATGCAGG CGGGCAGGGT 501 TCGCGGCAAA GGAAAAACCG CGCCTACCAG CATACAAGGG GTCAAACAAA 551 TCATCAAAGC CCTGCGTTCG GGCGAAGCAA CCATCGTCCT GCCCGACCAC 601 GTCCCCTCCC CTCAAGAAGG CGGGGAAGGC GTATGGGTGG ATTTCTTCGG 651 CAAACCTGCC TATACCATGA CGCTGGCGGC AAAATTGGCA CACGTCAAAG 701 GCGTGAAAAC CCTGTTTTTC TGCTGCGAAC GCCTGCCTGG CGGACAAGGT 751 TTCGATTTGC ACATCCGCCC CGTCCAAGGG GAATTGAACG GCGACAAAGC 801 CCATGATGCC GCCGTGTTCA ACCGCAATGC CGAATATTGG ATACGCCGTT 851 TTCCGACGCA GTATCTGTTT ATGTACAACC GCTACAAAAT GCCGTAA This corresponds to the amino acid sequence <SEQ ID 1374; ORF 505-l>: m505-l.pep 1 MFRLQFRLFP PLRTAMHILL TALLKCLSLL PLSCLHTLGN RLGHLAFYLL 51 KEDRARIVAN MRQAGLNPDP KTVKAVFAET AKGGLELAPA FFRKPEDIET 101 MFKAVHGWEH VQQALDKHEG LLFITPHIGS YDLGGRYISQ QLPFPLTAMY 151 KPPKIKAIDK IMQAGRVRGK GKTAPTSIQG VKQIIKALRS GEATIVLPDH 201 VPSPQEGGEG VWVDFFGKPA YTMTLAAKLA HVKGVKTLFF CCERLPGGQG 251 FDLHIRPVQG ELNGDKAHDA AVFNRNAEYW IRRFPTQYLF MYNRYKMP* m505-l/g505 94.3% identity in 298 aa overlap 10 20 30 40 50 60 m505-1.pep MFRLQFRLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGHLAFYLLKEDRARIVAN 111111111111 E I f i 111 i 11111 J 11 J J g505 MFRLQFRLFPPLRTAMHILLTALLKCLSLLSLSCLHTLGNRLGHLAFYLLKEDRARIVAN 10 20 30 40 50 60 70 80 90 100 110 120 m505-1.pep MRQAGLNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMFKAVHGWEHVQQALDKHEG 111111 i 111 II g505 MRQAGLNPDTQTVKAVFAETAKCGLELAPAFFKKPEDIETMFKAVHGWEHVQQALDKGEG 70 80 90 100 110 120 130 140 150 160 170 180 m505-l.pep LLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDKIMQAGRVRGKGKTAPTSIQG I i : i 111 I : g505 LLFITPHIGSYDLGGRYISQQLPFHLTAMYKPPKIKAIDKIMQAGRVRGKGKTAPTGIQG 130 140 150 160 170 180 190 200 210 220 230 240 m505-1.pep VKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGKPAYTMTLAAKLAHVKGVKTLFF I I I I I H I h l l l l M I I I I I I I I I I I I M M M l I I I I I I M M U g505 VKQIIKALRAGEATIILPDHVPSPQEGG-GVWADFFGKPAYTMTLAAKLAHVKGVKTLFF 190 200 210 220 230 250 260 270 280 290 299 m505-1.pep CCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRNAEYWIRRFPTQYLFMYNRYKMPX llll II g505 CCERLPDGQGFVLHIRPVQGELNGNKAHDAAVFNRNTEYWIRRFPTQYLFMYNRYKTPX 240 250 260 270 280 290 m505-l/a505 99.7% identity in 298 aa overlap 10 20 30 40 50 60 m505-1.pep MFRLQFRLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGHLAFYLLKEDRARIVAN a505 MFRLQFRLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGHLAFYLLKEDRARIVAN 10 20 30 40 50 60 70 80 90 100 110 120 m505-1.pep MRQAGLNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMFKAVHGWEHVQQALDKHEG a505 MRQAGMNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMFKAVHGWEHVQQALDKHEG 70 80 90 100 110 120 130 140 150 160 170 180 m505-l.pep LLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDKIMQAGRVRGKGKTAPTSIQG I I I I I I I I I I I I I M I I I I I I I I I M I I I M M M I I I M I I M I I I M I M M I I I I I I a505 LLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDKIMQAGRVRGKGKTAPTSIQG 130 140 150 160 170 180 190 200 210 220 230 240 m505-l .pep VKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGKPAYTMTLAAKLAHVKGVKTLFF a505 VKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGKPAYTMTLAAKLAHVKGVKTLFF 190 200 210 220 230 240 250 260 270 280 290 299 m505 -1. pep CCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRNAEYWIRRFPTQYLFMYNRYKMPX a505 CCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRNAEYWIRRFPTQYLFMYNRYKMPX 250 260 270 280 290 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1375>: g506. seq 1 ATGGCGGTAT TTGATGAAGT CGGGCGCATC GCCCATGGCT GCGGCGGTGT 51 TGTCAAACAA AGCCTGTTTC TGCGCGTCGT TCATCAGGTT GAACAAGGCG 101 CGCGGTTGGC TGAAGTAGTC GTCATCGTCT TGGCGGTAGT CCCAGTGTGC 151 CGCGTCGCCG TTGATTTTCA AAGGCGGTTC GGCGAAGTCG GGTTGTTGCT 201 GCCATTGGCC GAAGCTGTTG GGTTCGTAGT GCGGCAGGCT GCCGTAGTTG 251 CCGTCGGCGC GGCCTTGTCC GTCGCGCTGG TTGCTGTGAA CAGGGCAACG 301 CGGACGATTG ACGGGGATTT GGCGGAAGTT CACACCCAAG CGGTAACGTT 351 GCGCGTCGGC GTAATTGAAC AAACGGGCTT GCAACATTTT ATCCGGGCTC 401 GCGCCGATAC CGGGAACGAG GTTGCTCGGT GCGAAGGCGG ATTGTTCCAC 451 ATCGGCGAAG AAGTTTTCGG GATTGCGGTT CAACTCGAAT TCGCCCACTT 501 CAATCAGCGG ATAGTCTTTT TTCGGCCAAA CTTTGGTCAA GTCAAACGGA 551 TGATAAGGCA CTTTTTCGGC ATCGGCTTCA GGCATGACTT GGATGTACAT 601 CGTCCATTTC GGGAACTCGC CGCGCTCGAT GGCTTCGTAC AGGTCGCGCT 651 GATGGCTTTC GCGGTCGTCG GCGATGATTT TTGCAGCTTC TTCGTTGGTC 701 AGGTTTTTAA TCCCTTGCTG GCTGCGGAAA TGGAATTTCA CCCAAAAACG 751 TTCGCCCGCT TCGTTCCAGA AGCTGTAGGT ATGCGAACCG AAGCCGTGCA 801 TATGGCGGTA GCTGGCGGGA ATACCGCGGT CGCTCATCAC GATGGTAACT 851 TGGTGCAGGG CTTCGGGCAG CAGCGTCCAG AAGTCCCAGT TGTTTGTGGC 901 GGAACGCATA TTGGTGCGCG GATCGCGTTT GACGGCTTTG TTCAGGTCGG 951 GGAATTTGCG CGGGTCGCGC AGGAAGAACA CGGGCGTGTT GTTGCCGACC 1001 ACATCCCAGT TGCCTTCTTC GGTATAGAAT TTCAACGCAA AACCGCGGAT 1051 GTCGCGTTCC GCATCGGCTG CGCCGCGCTC GCCTGCCACG GTGGTGAAAC 1101 GGGCGAACAT CTCGGTTTTT TTGCCGACTT CGCTGAAAAT TTTGGCGCGG 1151 GTGTATTTGG TGATGTCGTG TGTTACGGTA AACGTACCGA ACGCGCCCGA 1201 ACCTTTGGCG TGCATACGGC GTTCGGGGAT GACTTCGCGC ACGAAGTCGG 1251 CGAGTTTTTC ATTCAGCCAC AAATCTTGCG TCAGCAGGGG GCCGCGCGGG 1301 CCGGCGGTCA GGCTGTTTTG ATTGTCGGCA ACGGGCGCGC CGTTGTTCAT 1351 GGTCAGATGG GTTACGGGGC ATTTGGAGGT AGTCATCGCT CTTGTTCCTT 1401 TTCTCAGGTT GGTCAAATGG GGGGCAAACG GCTTACAGTA CGATTTGGCG 1451 GAAAGCGTAT TCGTAACCGG TTTCTTGATT GTAATAAATT TCTTGAATCG 1501 ACATTTTATT TTCCTTTTGC AAAAACTATG GATGCGATTA TACGCCAAGA 1551 TTTTCGTTAT TAA This corresponds to the amino acid sequence <SEQ ID 1376; ORF 506.ng>: g506.pep 1 MAVFDEVGRI AHGCGGWKQ SLFLRWHQV EQGARLAEW VIVLAWPVC 51 RVAVDFQRRF GEVGLLLPLA EAVGFWRQA AWAVGAALS VALVAVΝRAT 101 RTIDGDLAEV HTQAVTLRVG VIEQTGLQHF IRARADTGΝE VARCEGGLFH 151 IGEEVFGIAV QLEFAHFΝQR IVFFRPΝFGQ VKRMIRHFFG IGFRHDLDVH 201 RPFRELAALD GFVQVALMAF AWGDDFCSF FVGQVFNPLL AAEMEFHPKT 251 FARFVPEAVG MRTEAVHMAV AGGNTAVAHH DGNLVQGFGQ QRPEVPWCG 301 GTHIGARIAF DGFVQVGEFA RVAQEEHGRV VADHIPVAFF GIEFQRKTAD 351 VAFRIGCAAL ACHGGETGEH LGFFADFAEN FGAGVFGDW CYGKRTERAR 401 TFGVHTAFGD DFAHEVGEFF IQPQILRQQG AARAGGQAVL IVGNGRAWH 451 GQMGYGAFGG SHRSCSFSQV GQMGGKRLTV RFGGKRIRNR FLDCNKFLES 501 TFYFPFAKTM DAIIRQDFRY * The following partial DNA sequence was identified in N meningitidis <SEQ ID 1377>: m506. seq 1 ATGGCGGTAT TTGATGAAGT CGGGCGCGTC GCCCATTGCG GCGGCGGTGT 51 TGCCGAACAA TGCCTGTTTC TGCGCGTCGT TCATCAGGTT GAACAGGGCG 101 CGCGGTTGGC TGAAATAGTC GTCATCGTCT TGGCGGTAGT CCCAGTGTGC 151 CGCGTCGCCG TTGATTTTCA AAGGCGGTTC GGCGAAtCGg GGTTGTTGCT 201 GCCATTGGCC GAAGCTGTyG GGTTCGTAGT GCGGCAGGCT GCCGyAGTTG 251 CCGTCGGCGC GGCCTTGCCC GTyGCGsTgr TTgCTGTgAA CAsGGCAACG 301 CGGACGATTG ACGGGAATTT GGCGGAAGTT TACGCCCAAA CGGTAGCGTT 351 GTGCGTCGGC GTAATTGAAC AAACGCGCTT GCAGCATTTT ATCTsGGCTG 401 GCGCCGACAC CGGGAACGAG GTTGCTCGGT GCGAAGGCGG ATTGTTCCAC 451 ATCGGCGAAG AAGTTTTCGG GATTGCGGTT CTCAAACGGA TGATAAGGTA 501 CTTTTTCCGC GTCTGCTTCA GGCATGACTT GGATGTACAT CGTCCATTTC 551 GGAAACTCGC CGCGTTCGAT GGCTTCsTAT AAGTCGCGCT GATGGCTTTC 601 GCGGTCGTCG GCGATGATTT TGGCGGCTTC TTCGTTGGTC AGGTTTTTAA 651 TGCCTTGTTG GGTGCGGAAA TGGAATTTCA CCCAAAAACG CTCGCCTGCT 701 TCGTTCCAGA AGCTGTAGGT ATGCGAACCG AAGCCGTGCA TATGGCGGTA 751 GCCGGCGGGG ATGCCGCGGT CGCTCATCAC GATGGTAACT TGGTGCAGTG 801 CTTCGGGCAG CAGCGTCCAG AAGTCCCAGT TGTTTGTGGC AGAGCGCATA 851 TTGGTGCGCG GGTCGCGTTT GACGGCTTTG TTCAGGTCGG GGAACTTACG 901 CGGGTCGCGC AGGAAGAACA CGGGCGTGTT GTTGCCGACC ACATCCCAGT 951 TGCCTTCTTC GGTATAAAAT TTCAAGGCAA AACCGCGGAT GTCGCGTTCT 1001 GCATCGGCTG CGCCGCGTTC GCCTGCCACG GTGGTGAAAC GGGCGAACAT 1051 CTCGGTTTTT TTGCCGACTT CGCTGAAGAT TCCTTTGGCG TGCATACGGC 1101 GTTCGGGGAT GACTTCGCGC ACGAAGTCGG CGAGTTTTTC AGTCATCGCT 1151 CTTGTTCCTT TTCTCAGGTT GGTCAAATGG GGGTAAACGG CTTACAGTAC 1201 GATTTGGCGG AAAGCGTATT CGTAACCGGT TTCTTGATTG CAATAAATTT 1251 CTTGAATCGA CATTTTATTT CCCTTTTGTA AAAACTATGG ATGCGACTAT 1301 ACGCCAAGAT TTTCGCTATT AA This corresponds to the amino acid sequence <SEQ ID 1378; ORF 506>: m506.pep 1 MAVFDEVGRV AHCGGGVAEQ CLFLRWHQV EQGARLAEIV VIVLAWPVC 51 RVAVDFQRRF GESGLLLPLA EAVGFWRQA AXVAVGAALP VAXXAVΝXAT 101 RTIDGΝLAEV YAQTVALCVG VIEQTRLQHF IXAGADTGΝE VARCEGGLFH 151 IGEEVFGIAV QLEFAHFΝQR IVFFRPΝFGQ VKRMIRYFFR VCFRHDLDVH 201 RPFRKLAAFD GFXXVALMAF AWGDDFGGF FVGQVFΝALL GAEMEFHPKT 251 LACFVPEAVG MRTEAVHMAV AGGDAAVAHH DGΝLVQCFGQ QRPEVPWCG 301 RAHIGARVAF DGFVQVGELT RVAQEEHGRV VADHIPVAFF GIKFQGKTAD 351 VAFCIGCAAF ACHGGETGEH LGFFADFAED FGAGVFGDW RYGKRTERAR 401 TFGVHTAFGD DFAHEVGEFF IQPQILRQQR AARTGGQAVL IVGΝRRAWH 451 GQMGYRAFGG SHRSCSFSQV GQMGGKRLTV RFGGKRIRNR FLDCNKFLES 501 TFYFPFVKTM DATIRQDFRY * Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 506 shows 89.2% identity over a 520 aa overlap with a predicted ORF (ORF 506.ng) from N gonorrhoeae: m506/g506 10 20 30 40 50 60 m506. pep MAVFDEVGRVAHCGGGVAE Q CLFLRWHQVEQGARLAEIWIVLAWPVCRVAVDFQRRF I M M M M M I l l l - l I I 1 1 I I I I I I I I I I 1 1 h I I I I I I I I I I I I I I I I I 1 1 I I g506 MAVFDEVGRIAHGCGGWKQSLFLRWHQVEQGARLAEVWIVLAWPVCRVAVDFQRRF 10 20 30 40 50 60 70 80 90 100 110 120 m506. pep GESGLLLPLAEAVGFWRQAAXVAVGAALPVAXXAVNXATRTIDGNLAEVYAQTVALCVG II IMMMMIMMMM 11 ! ! 111 II Ml IMMIhlllhMMM II g 506 GEVGLLLPLAEAVGFWRQAAWAVGAALSVALVAVNRATRT I DGDLAEVHTQAVTLRVG 70 80 90 100 110 120 130 140 150 160 170 180 m506. pep VIEQTRLQHFIXAGADTGNEVARCEGGLFHIGEEVFGIAVQLEFAHFNQRIVFFRPNFGQ Mill Mill I MMMMMMMMMMMMMMMMIMMMIMMM g506 VIEQTGLQHFIRARADTGNEVARCEGGLFHIGEEVFGIAVQLEFAHFNQRIVFFRPNFGQ 130 140 150 160 170 180 190 200 210 220 230 240 m506.pep VKRMIRYFFRVCFRHDLDVHRPFRKLAAFDGFXXVALMAFAWGDDFGGFFVGQVFNALL MM- : I M 1111 II 111 = 111 = I II MMMMIMM =11111111 II g506 VKRMIRHFFGIGFRHDLDVHRPFRELAALDGFVQVALMAFAWGDDFCSFFVGQVFNPLL 190 200 210 220 230 240 250 260 270 280 290 300 m506. pep GAEMEFHPKTLACFVPEAVGMRTEAVHMAVAGGDAAVAHHDGNLVQCFGQQRPEVPWCG MMMMMMMMMIhMMIMIMM g506 AAEMEFHPKTFARFVPEAVGMRTEAVHMAVAGGNTAVAHHDGNLVQGFGQQRPEVPWCG 250 260 270 280 290 300 310 320 330 340 350 360 m506.pep RAHIGARVAFDGFVQVGELTRVAQEEHGRWADHIPVAFFGIKFQGKTADVAFCIGCAAF :| 111 I II 111111 = l 111111 II 111111 II 111 II UI Mllh g506 GTHIGARIAFDGFVQVGEFARVAQEEHGRWADHIPVAFFGIEFQRKTADVAFRIGCAAL 310 320 330 340 350 360 370 380 390 400 410 420 m506. pep ACHGGETGEHLGFFADFAEDFGAGVFGDWRYGKRTERARTFGVHTAFGDDFAHEVGEFF I ] F E ! 11 E 11111111111 = 111 ! 111111 111 ! 1111111 E 1 E 111111 E 11111 E 11 g506 ACHGGETGEHLGFFADFAENFGAGVFGDWCYGKRTERARTFGVHTAFGDDFAHEVGEFF 370 380 390 400 410 420 430 440 450 460 470 480 m506. pep IQPQILRQQRAARTGGQAVLIVGNRRAWHGQMGYRAFGGSHRSCSFSQVGQMGGKRLTV g506 IQPQILRQQGAARAGGQAVLIVGNGRAWHGQMGYGAFGGSHRSCSFSQVGQMGGKRLTV 430 440 450 460 470 480 490 500 510 520 m506.pep RFGGKRIRNRFLDCNKFLESTFYFPFVKTMDATIRQDFRY MM MM MM I MMM M I g506 RFGGKRIRNRFLDCNKFLESTFYFPFAKTMDAIIRQDFRY 490 500 510 520 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1379>: a506. seq 1 ATGGCGGTAT TTGATGAAGT CGGGCGCGTC GCCCATTGCG GCGGCGGTGT 51 TGCCGAACAA TGCCTGTTTC TGCGCGTCGT TCATCAGGTT GAACAGGGCG 101 CGCGGTTGGC TGAAATAGTC GTCATCGTCT TGGCGGTAGT CCCAGTGCGC 151 CGCGTCGCCG TTGATTTTCA AAGGCGGTTC GGCGAAGTCG GGCTGCTGCT 201 GCCATTGGCC GAAGCTGTTG GGTTCGTAGT GCGGCAGGCT GCCGTAGTTG 251 CCGTCGGCGC GTCCTTGTCC GTCGCGCTGG TTGCTGTGAA CAGGGCAACG 301 CGGACGGTTG ACAGGGATTT GGCGGAAGTT CACGCCCAAG CGGTAGCGTT 351 GCGCGTCGGC GTAATTGAAC AAACGCGCCT GCAACATTTT ATCTGGGCTG 401 GCGCCGACAC CGGGAACGAG GTTGCTCGGT GCGAAGGCGG ATTGTTCCAC 451 ATCGGCGAAG AAGTTTTCGG GATTGCGGTT CAACTCGAAT TCGCCCACTT 501 CAATCAGCGG ATAGTCTTTT TTCGGCCAAA CTTTGGTCAA GTCAAACGGA 551 TGATACGGCA CTTTTTCCGC ATCGGCTTCA GGCATGACTT GGATGTACAT 601 CGTCCATTTC GGAAACTCGC CGCGCTCGAT GGCTTCGTAC AGGTCGCGCT 651 GATGGCTTTC ACGGTCGTCG GCGATGATTT TGGCGGCTTC TTCGTTGGTC 701 AGGTTTTTAA TGCCTTGTTG GGTGCGGAAA TGGAATTTCA CCCAAAAACG 751 CTCGCCTGCT TCGTTCCAGA AGCTGTAGGT ATGCGAACCG AAGCCGTGCA 801 TATGGCGGTA GCCGGCGGGG ATGCCGCGGT CGCTCATCAC GATGGTAACT 851 TGGTGCAGTG CTTCGGGCAG CAGCGTCCAG AAGTCCCAGT TGTTTGTGGC 901 AGAGCGCATA TTGGTGCGCG GGTCGCGTTT GACGGCTTTG TTCAGGTCGG 951 GGAACTTACG CGGGTCGCGC AGGAAGAACA CGGGCGTGTT GTTGCCGACC 1001 ACATCCCAGT TGCCTTCTTC GGTATAGAAC TTCAACGCAA AACCGCGGAT 1051 GTCGCGTTCT GCATCGGCTG CGCCGCGTTC GCCTGCCACG GTGGTGAAAC 1101 GGGCGAACAT CTCGGTTTTT TTGCCGACTT CGCTGAAGAT TTTGGCGCGG 1151 GTGTATTTGG TGATGTCGTG CGTTACGGTA AACGTACCGA ACGCGCCCGA 1201 ACCTTTGGCG TGCATACGGC GTTCGGGGAT GACTTCGCGC ACGAAGTCGG 1251 CGAGTTTTTC ATTCAGCCAC AAATCCTGCG CCAGCAGAGG GCCGCGAGGA 1301 CCGGCGGTCA GGCTGTTTTG ATTGTCGGCA ACAGGCGCGC CGTTGTTCAT 1351 GGTCAGATGG GTTACAGGGC ATTTGGAGGT ANTCATCGCT CTTGTTCCTT 1401 TTCTCAGGTT GGTCAAAT . G GGGGTAAACG GCTTACAGTA CGATTTGGCG 1451 GAAAGCGTAT TCGTAACCGG TTTCTTGATT GCAATAAATT TCTTGAATCG 1501 ACATTTTATT TCCCTTTTGT AAAAACTATG GATGCGACTA TACGCCAAGA 1551 TTTTCGCTAT TAA This corresponds to the amino acid sequence <SEQ ID 1380; ORF 506. a>: a506.pep 1 MAVFDEVGRV AHCGGGVAEQ CLFLRVVHQV EQGARLAEIV VIVLAVVPVR 51 RVAVDFQRRF GEVGLLLPLA EAVGFWRQA AVVAVGASLS VALVAVNRAT 101 RTVDRDLAEV HAQAVALRVG VIEQTRLQHF IWAGADTGNE VARCEGGLFH 151 IGEEVFGIAV QLEFAHFNQR IVFFRPNFGQ VKRMIRHFFR IGFRHDLDVH 201 RPFRKLAALD GFVQVALMAF TVVGDDFGGF FVGQVFNALL GAEMEFHPKT 251 LACFVPEAVG MRTEAVHMAV AGGDAAVAHH DGNLVQCFGQ QRPEVPWCG 301 RAHIGARVAF DGFVQVGELT RVAQEEHGRV VADHIPVAFF GIELQRKTAD 351 VAFCIGCAAF ACHGGETGEH LGFFADFAED FGAGVFGDW RYGKRTERAR 401 TFGVHTAFGD DFAHEVGEFF IQPQILRQQR AARTGGQAVL IVGNRRAVVH 451 GQMGYRAFGG XHRSCSFSQV GQXGGKRLTV RFGGKRIRNR FLDCNKFLES 501 TFYFPFVKTM DATIRQDFRY * m506/a506 94.8% identity in 520 aa overlap 10 20 30 40 50 60 m506. pep MAVFDEVGRVAHCGGGVAEQCLFLRWHQVEQGARLAEIWIVLAWPVCRVAVDFQRRF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I llllllllll a506 MAVFDEVGRVAHCGGGVAEQCLFLRWHQVEQGARLAEIVVIVLAWPVRRVAVDFQRRF 10 20 30 40 50 60 70 80 90 100 110 120 m506. pep GESGLLLPLAEAVGFWRQAAXVAVGAALPVAXXAVNXATRTIDGNLAEVYAQTVALCVG II I I I I I I I I I I I I I I I I I I I I I I I : I II III I I I I : I : I I I I : I I : I I I II a506 GEVGLLLPLAEAVGFWRQAAVVAVGASLSVALVAVNRATRTVDRDLAEVHAQAVALRVG 70 80 90 100 110 120 130 140 150 160 170 180 m506.pep VIEQTRLQHFIXAGADTGNEVARCEGGLFHIGEEVFGIAVQLEFAHFNQRIVFFRPNFGQ I I I II I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a506 VIEQTRLQHFIWAGADTGNEVARCEGGLFHIGEEVFGIAVQLEFAHFNQRIVFFRPNFGQ 130 140 150 160 170 180 190 200 210 220 230 240 m506.pep VKRMIRYFFRVCFRHDLDVHRPFRKLAAFDGFXXVALMAFAVVGDDFGGFFVGQVFNALL lllll ll I I I I I I I I I I I I I I I I : I I I I I I I I I : I I I I I I I I I I I I I I I I I I I a506 VKRMIRHFFRIGFRHDLDVHRPFRKLAALDGFVQVALMAFTWGDDFGGFFVGQVFNALL 190 200 210 220 230 240 250 260 270 280 290 300 m506. pep GAEMEFHPKTLACFVPEAVGMRTEAVHMAVAGGDAAVAHHDGNLVQCFGQQRPEVPVVCG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M I I a506 GAEMEFHPKTLACFVPEAVGMRTEAVHMAVAGGDAAVAHHDGNLVQCFGQQRPEVPWCG 250 260 270 280 290 300 310 320 330 340 350 360 m506.pep RAHIGARVAFDGFVQVGELTRVAQEEHGRWADHIPVAFFGIKFQGKTADVAFCIGCAAF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : : I llllllllllllll a506 RAHIGARVAFDGFVQVGELTRVAQEEHGRVVADHIPVAFFGIELQRKTADVAFCIGCAAF 310 320 330 340 350 360 370 380 390 400 410 420 m506.pep ACHGGETGEHLGFFADFAEDFGAGVFGDWRYGKRTERARTFGVHTAFGDDFAHEVGEFF I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a506 ACHGGETGEHLGFFADFAEDFGAGVFGDVVRYGKRTERARTFGVHTAFGDDFAHEVGEFF 370 380 390 400 410 420 430 440 450 4 60 470 480 m506 . pep IQPQILRQQRAARTGGQAVLIVGNRRAWHGQMGYRAFGGSHRSCSFSQVGQMGGKRLTV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I l l l l l l l a506 IQPQILRQQRAARTGGQAVLIVGNRRAWHGQMGYRAFGGXHRSCSFSQVGQXGGKRLTV 430 440 450 460 470 480 490 500 510 520 m506.pep RFGGKRIRNRFLDCNKFLESTFYFPFVKTMDATIRQDFRYX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a506 RFGGKRIRNRFLDCNKFLESTFYFPFVKTMDATIRQDFRYX 490 500 510 520 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1381>: g507.seq 1 ATGCTCTTGC CGGCTTTGCA ACAAGGCGGC GGCTTCCTGA GCGGCGGCGG 51 TTTCGGCCTC GTCGGGCAGG TTCAGGGCTT GGTTTTCCTG CTTCAGACGG 101 CCTTTGCGCT CTTCGTGCTT GGCAACGGTT TGTTCGGCAT GGGCAAGCTG 151 CTGCTGCTTC AACGCCAGTT CGCGGCGGAT GCGGTTTGCC TCGTCCTGCT 201 GGGTTTGGAA GGCAGCGTTG AGCGTGGCTT GGACTTCTTC CAATTCGGGC 251 AGACGCTCTT CGTGTTCGGC AACCTGCATC GCCCATTCCG CCAATTCGGT 301 TTGCTTTTCT TCGACCTGCA ACTCGTTTTC CTCAAGCTGC ACGCGGATTT 351 GCTGCTGCTC CTGCCGGATG CGTTGCAACT GCGCCTGCGC TGCCTGCTTG 401 TCGCGTTCGA TGCGTTGGTG CAGGTTTTGC CGGTGGCGGA TTTGTTCTTC 451 CAAACGGGCA ATCTGCTCGC GCAACACGCC GCGTTTGTTG CTCAATTCGT 501 GTACTGCCTG CTGCTGCGAC TGTTCGGCAG TCTGCAAGGC GTGTACTTCG 551 TTATTTAA This corresponds to the amino acid sequence <SEQ ID 1382; ORF 507.ng>: g507.pep 1 MLLPALQQGG GFLSGGGFGL VGQVQGLVFL LQTAFALFVL GΝGLFGMGKL 51 LLLQRQFAAD AVCLVLLGLE GSVERGLDFF QFGQTLFVFG ΝLHRPFRQFG 101 LLFFDLQLVF LKLHADLLLL LPDALQLRLR CLLVAFDALV QVLPVADLFF 151 QTGΝLLAQHA AFVAQFVYCL LLRLFGSLQG VYFVI* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1383>: m507. seq 1 ATGCTCTTGC TGACTTTGCA ACAAGGCGGC TGCTTCCTGC GCGGCGGCGG 51 TTTCGGCTTC GTCGGGCAGG TTTAAGGCTT GGTTTTCCTG TTTCAGACGA 101 CCTTTGCGCT CTTCGTGCTT GGCAATCGTT TGTTCGGCAT GGGCAAGCTG 151 CTGCTGCTTC AACGCCAGTT CGCGGCGGAT GCGGTTTGCC TCGTCCTGCT 201 GGGTTTGGAA GGCGGCGTTG AGCGTGGCTT GGGCTTCTTC CAATTCGGGC 251 AGACGCTCCT CGTGTTCGGC AACCTGCATC GCCCATTCCG CCAGCTCGGT 301 TTGTTTTTCT TCGACCTGCA ACTCGTTTTC TTCAAGCTGC ACGCGGATTT 351 GCTGCTGCTC TTGATGAATG CGTTGTAACT GCGCCTGCGC TGCCTGCTTG 401 TCGCGTTCGA TGCGTTGGTG CAGGTTTTGC TGATGGCGGA TTTGTTCTTC 451 CAAACGGGCA ATCTGCTCGC GCAACACGCC GCGCTTGTTG CTCAATTCAT 501 GCACTGCCTG CTGCTGCGAC TGTTCGGCAG TCTGCAAGGC GTGTACTTCG 551 TCGTTTAA This corresponds to the amino acid sequence <SEQ ID 1384; ORF 507>: m507.pep 1 MLLLTLQQGG CFLRGGGFGF VGQVXGLVFL FQTTFALFVL GΝRLFGMGK 51 LLLQRQFAAD AVCLVLLGLE GGVERGLGFF QFGQTLLVFG NLHRPFRQLG 101 LFFFDLQLVF FKLHADLLLL LMNALXLRLR CLLVAFDALV QVLLMADLFF 151 QTGNLLAQHA ALVAQFMHCL LLRLFGSLQG VYFW* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 507 shows 87.0% identity over a 185 aa overlap with a predicted ORF (ORF 507.ng) from N gonorrhoeae: m507/g507 10 20 30 40 50 60 m507. pep MLLLTLQQGGCFLRGGGFGFVGQVXGLVFLFQTTFALFVLGΝRLFGMGKLLLLQRQFAAD Ml MMM II Mllhllll MMMMMIMMM g507 MLLPALQQGGGFLSGGGFGLVGQVQGLVFLLQTAFALFVLGΝGLFGMGKLLLLQRQFAAD 10 20 30 40 50 60 70 80 90 100 110 120 m507. pep AVCLVLLGLEGGVERGLGFFQFGQTLLVFGΝLHRPFRQLGLFFFDLQLVFFKLHADLLLL 11111111111 = 11111 II Ml MMM III MM III III g507 AVCLVLLGLEGSVERGLDFFQFGQTLFVFGΝLHRPFRQFGLLFFDLQLVFLKLHADLLLL 70 80 90 100 110 120 130 140 150 160 170 180 m507. pep LMΝALXLRLRCLLVAFDALVQVLLMADLFFQTGΝLLAQHAALVAQFMHCLLLRLFGSLQG I Ml MMMMMMMMIMMhMMMIMMM g507 LPDALQLRLRCLLVAFDALVQVLPVADLFFQTGΝLLAQHAAFVAQFVYCLLLRLFGSLQG 130 140 150 160 170 180 m507.pep VYFW lllh g507 VYFVI The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1385>: a507.seq 1 ATGCTCTTGC TGGCTTTGCA ACAAGGCGGC AGCTTCCTGC GCGGCGGCGG 51 TTTCGGCTTC GTCAGGCAGA TTCAGGGCTT GGTTTTCCTG TTTCAGACGA 101 CCTTTGCGCT CTTCGTGCTT GGCAACGGTT TGTTCGGCAT GGGCAAGCTG 151 CTGCTGCTTC AACGCCAGTT CGCGGCGGAT GCGGTTTGCC TCGTCCTGCT 201 GGGTTTGGAA GGCGGCATTG AGTGTGGCTT GGGTTTCTTC CAATTCGGGC 251 AGACGCTCTT CGTGTTCGGC AACCTGCATC GCCCATTCCG CCAATTCGGT 301 TTGCTTTTCT TCCGCCTGCA ACTCGTTTTC TTCAAGCTGC ACGCGGATTT 351 GCTGCTGCTC CTGATGGATG CGCTGCATCT GCGCCTGCGC CGCCTGCTTG 401 TCGCGTTCGA TGCGTTGGTG CAGGTTTTGC TGATGGCGGA TTTGTTCTTC 451 CAAACGGGCA ATCTGTTCGC GCAACACGCC GCGTTTGTTG CCCAATTCGT 501 GCACCGCCTG CTGCTGCGAC TGTTCGGCAG TCTGCAAGGC GTGTACTTCG 551 TCGTTTAA This corresponds to the amino acid sequence <SEQ ID 1386; ORF 507. a>: a507.pep 1 MLLLALQQGG SFLRGGGFGF VRQIQGLVFL FQTTFALFVL GΝGLFGMGKL 51 LLLQRQFAAD AVCLVLLGLE GGIECGLGFF QFGQTLFVFG ΝLHRPFRQFG 101 LLFFRLQLVF FKLHADLLLL LMDALHLRLR RLLVAFDALV QVLLMADLFF 151 QTGΝLFAQHA AFVAQFVHRL LLRLFGSLQG VYF * m507/a507 89.7% identity in 185 aa overlap 10 20 30 40 50 60 m507. pep MLLLTLQQGGCFLRGGGFGFVGQVXGLVFLFQTTFALFVLGΝRLFGMGKLLLLQRQFAAD lll lllll llllllllll I : lllllllllllllllll lllllllllllllllll a507 MLLLALQQGGSFLRGGGFGFVRQIQGLVFLFQTTFALFVLGΝGLFGMGKLLLLQRQFAAD 10 20 30 40 50 60 70 80 90 100 110 120 m507. pep AVCLVLLGLEGGVERGLGFFQFGQTLLVFGNLHRPFRQLGLFFFDLQLVFFKLHADLLLL I I I I I I I I I I I I : I I I I I I I I I I I I : I I I I I I I I I I I : I I : II lllllllllllllll a507 AVCLVLLGLEGGIECGLGFFQFGQTLFVFGNLHRPFRQFGLLFFRLQLVFFKLHADLLLL 70 80 90 100 110 120 130 140 150 160 170 180 m507. pep LMNALXLRLRCLLVAFDALVQVLLMADLFFQTGNLLAQHAALVAQFMHCLLLRLFGSLQG I I : I I llll I I I I II I I I I I I I I I I I I I I I I I I : I I I I I : I I I I : I I I I I I I I I I I I a507 LMDALHLRLRRLLVAFDALVQVLLMADLFFQTGNLFAQHAAFVAQFVHRLLLRLFGSLQG 130 140 150 160 170 180 m507.pep VYFVVX llllll a507 VYFWX The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1387>: g508. seq 1 ATGGTAGCGT TTGGCGTTGA TCAGGGCCTC CTGCTGCTGC AACAGGGCGG 51 TTTGGGTGGC GGCCTGAAGC TGCGGCAGCT TGGTTTGCAG GGTTTGTACG 101 CGGGCGTATT GCTCCCTGCC CTGTTCCTGA ATCTGCGCGA GTTTTTCCTG 151 CACGGCGATG TATTCTTCGT CCAGCGTGTG TACGGTTTCG GTCAACTCGT 201 CGAGCTTGAT GTGCTGCTCG TCGTTTTGGA ACTCGGTTTC ATAGGCGAGG 251 GCAAGCTCTT GCCGGCGTTC CTGCCAGTCC AGGGTTTGCT GTTCGAGCCG 301 GGCGATTTGC TGCCGGTAGT TTTGTTTTTG CGGGTTGAGT TTGTGGACGG 351 CGACTTCGGC AAGCCCGTAT TGGCGGTTGG CTTCCAACAG GGCAAGCTGC 401 GCCTGTTTCA GACGGCCTTG CTGCTCTTGG CGGCTGTGCG CGGTGGTTTG 451 CTGCTGGTGT TCGAGTTCGG CGGCGGCTTC CTGCAAAGTA GCGATGTCGT 501 CTGA This corresponds to the amino acid sequence <SEQ ID 1388; ORF 508.ng>: g508.pep 1 MVAFGVDQGL LLLQQGGLGG GLKLRQLGLQ GLYAGVLLPA LFLΝLREFFL 51 HGDVFFVQRV YGFGQLVELD VLLWLELGF IGEGKLLPAF LPVQGLLFEP 101 GDLLPWLFL RVEFVDGDFG KPVLAVGFQQ GKLRLFQTAL LLLAAVRGGL 151 LLVFEFGGGF LQSSDW The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 1389>: m508. seq 1 ATGGTAGCGT TTGGCGTTGA TCAGGGCTTC CTGCTGCTGC AACAAGGCGG 51 TTTGGGTGGC GGCCTGAAGC TGCGGCAGCT TGGTTTGCAG GGTTTGCACT 101 TTAGCGTATT GCTCCCTGCC CTGTTCCTGA ATCTGCGCGA GTTTCTCTTG 151 CACAACAATA TATTCTTCGT CCAAGGTCTG TACGGCTTCG CTTAATTCTT 201 CAAGCTTGAT GTGCTGCTCG TCGTTTTGGA ACTCGGTTTC ATAGGCGAGG 251 GCAAGCTCTT GCTGGCGTTC CTGCCAGTCG AGGGTTTGCT GTTCAAGCTG 301 GGCGATTTGC TGCCGGTAGT TTTGTTTTTG CTGGTTGAGT TTGTGGACGG 351 CGACTTCGGC AAGCCCGTAT TGGCGGTTGG CTTCCAACAG GGCAAGCTGC 401 GCCTGTTTCA GACGGCCTTG CTGCTCTTGG CGGCTGTGCG CGGTGGTTTG 451 CTGCTGGTGT TCGAGTTCGG CGGCGGCTTC CTGCAAGGTA ACGATGTCGT 501 CTGA This corresponds to the amino acid sequence <SEQ ID 1390; ORF 508.ng>: m508.pep 1 MVAFGVDQGF LLLQQGGLGG GLKLRQLGLQ GLHFSVLLPA LFLΝLREFLL 51 HΝΝIFFVQGL YGFAXFFKLD VLLWLELGF IGEGKLLLAF LPVEGLLFKL 101 GDLLPWLFL LVEFVDGDFG KPVLAVGFQQ GKLRLFQTAL LLLAAVRGGL 151 LLVFEFGGGF LQGΝDW* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 508 shows 86.8% identity over a 167 aa overlap with a predicted ORF (ORF 508.ng) from N gonorrhoeae: m508/g508 10 20 30 40 50 60 m508. pep MVAFGVDQGFLLLQQGGLGGGLKLRQLGLQGLHFSVLLPALFLNLREFLLHNNIFFVQGL MMMMIMMMMMMMIMMMIh M I II I II II II 11 M I = = M II I ■ ■ g508 MVAFGVDQGLLLLQQGGLGGGLKLRQLGLQGLYAGVLLPALFLNLREFFLHGDVFFVQRV 10 20 30 40 50 60 70 80 90 100 110 120 m508. pep YGFAXFFKLDVLLWLELGFIGEGKLLLAFLPVEGLLFKLGDLLPWLFLLVEFVDGDFG MM : MMMMMMMMMM IIIIIMMh g508 YGFGQLVELDVLLWLELGFIGEGKLLPAFLPVQGLLFEPGDLLPWLFLRVEFVDGDFG 70 80 90 100 110 120 130 140 150 160 m508. pep KPVLAVGFQQGKLRLFQTALLLLAAVRGGLLLVFEFGGGFLQGNDW MIMMMMMMMMMMMMMMMMIMMIhMM g508 KPVLAVGFQQGKLRLFQTALLLLAAVRGGLLLVFEFGGGFLQSSDW 130 140 150 160 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1391>: a508. seq 1 ATGGTAGCGT TTGGCGTTGA TCAGGGCTTC CTGCTGCTGC AACAGGGCGG 51 TTTGGGTGGC GGCCTGAAGC TGCGGCAGCT TGGTTTGCAG GGTTTGTACG 101 CGGGCGTATT GTTCCCTACC CTGCTCCTGA ATCTGCGCGA GTTTCTCCTG 151 TACGACAATA TATTCTTCGT CCAAACTCTG TACGGCTTCG CTCAACTCTT 201 CGAGCTTGAT GTGCTGCTCG TCGTTTTGGA ACTCGGTTTC ATAGGCGAGG 251 GCAAGCTCTT GCTGGCGTTC CTGCCAATCG AAGGTTTGTT GTTCAAGCTG 301 GGCAATTTGC TGTTGGTAGT TTTGTTTTTG CTGGTTGAGC TTGTGGACGG 351 CGACTTCGGC AAGCCCGTAT TGGCGGTTGG CTTCCAACAG GGCAAGCTGC 401 GCCTGTTTCA GACGACCTTG CTGCTCTTGG CGGCTGTGCG CGGCGGTTTG 451 CTGCTGGTGT TCGAGTTCGG CGGCGGCTTC CTGCAAAATG GCGATGTCGT 501 CTGA This corresponds to the amino acid sequence <SEQ ID 1392; ORF 508. a>: a508.pep 1 MVAFGVDQGF LLLQQGGLGG GLKLRQLGLQ GLYAGVLFPT LLLΝLREFLL 51 YDΝIFFVQTL YGFAQLFELD VLLWLELGF IGEGKLLLAF LPIEGLLFKL 101 GΝLLLWLFL LVELVDGDFG KPVLAVGFQQ GKLRLFQTTL LLLAAVRGGL 151 LLVFEFGGGF LQΝGDVV* m508/a508 88.6% identity in 167 aa overlap 10 20 30 40 50 60 m508. pep MVAFGVDQGFLLLQQGGLGGGLKLRQLGLQGLHFSVLLPALFLΝLREFLLHΝΝIFFVQGL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : : I I : I : I : I I I I I I I I :: I I I I I I I a508 MVAFGVDQGFLLLQQGGLGGGLKLRQLGLQGLYAGVLFPTLLLΝLREFLLYDΝIFFVQTL 10 20 30 40 50 60 70 80 90 100 110 120 m508. pep YGFAXFFKLDVLLVVLELGFIGEGKLLLAFLPVEGLLFKLGDLLPWLFLLVEFVDGDFG llll : I : I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I : I I I I I I I I I I : I I I I I I a508 YGFAQLFELDVLLWLELGFIGEGKLLLAFLPIEGLLFKLGΝLLLWLFLLVELVDGDFG 70 80 90 100 110 120 130 140 150 160 m508. pep KPVLAVGFQQGKLRLFQTALLLLAAVRGGLLLVFEFGGGFLQGΝDWX I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I : : I I I I a508 KPVLAVGFQQGKLRLFQTTLLLLAAVRGGLLLVFEFGGGFLQΝGDWX 130 140 150 160 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 1393>: g509. seq 1 atggtcgctg tatgtgatga acgggctgta cagcggacgt tggtggccca 51 attcgcgcaa caaggcggct tgtttttgct cttcgttcag gctgttgtag 101 tcttccaagc ctgcgtgttg gaaaagctcg gcaaccacat cggcgtgttt 151 gcctgcgtgt tggcgcaggt cgagcggcat catgtggaag ccgaacacgg 201 acacggaacg gatgaggtct gccaaacggc cttcggcaag caggcggctg 251 ccgttgtcga taagggaacg ttgcaatttt ttcaaatcat cgagaaattt 301 ttgggccgaa gcataaggct cgagaaagcc gaatttgcag cccatgccca 351 aaccgagcga gcgcgctttg cccatagcgc gcgccataat gtaggcaatg 401 gcgcggcggt aaggttcttc ggtgcgggcg atttcttcgt caggcgagag 451 ggctgccagt gccattacgt cgtcgttgac tttgacgcgg cggatggaaa 501 gcggcagttc gcggtaaagt ttgtcgagtt cgctgcggta aaaacggaac 551 acggcatcgg cgtggcggcg gaaggcaaag cgcagggttt cgccagaaac 601 aaacggattg ccgtcgcggt cgccgccgat ccagccgccg attttaagga 651 tattcggaac gcggacatcg ggataggccg tctgaaagtc gtgttccatc 701 ttgcggtaga gtttgggcag ggcttcaaaa aagctcatcg ggaagatgga 751 cacgccgttg ttgatttcgt cgttgacgct gagtttgtgg cggcgcgttt 801 cgctggtctg ccacaagccc agaagcacgg tgtcgatttc gcggcgcagc 851 cgtgccagcg cgtcggcatt ggtgcagcgt tcgcgttgcg gcagcagcgc 901 gcggatgcgg cggttgaaat tcaaaacggt ttggcgttgc acttcggtcg 951 ggtgcgcggt caaaacggcg gtaacggacg tattgtccaa ctgccgctgc 1001 accgatttgc cgtcggcttt ccccgctttg agcctgcgga cggtttccgt 1051 caggctgcct tctgctgcgt tgtggccggc atcttcgtgg atttggcggc 1101 ggcgttcgtg gtgcacgtct tcggcgatat tcagaatctg ggcgaacagc 1151 ccgcaggcaa gcgtcagatc gtaggtctgc cgttcgtcca attgcggcaa 1201 tactttttca atcaatgccg cgctgtcgtc ggaagtggac aagagtttga 1251 ccgtttcgac aaccaacggc gaggcttctt cgtgcaggag gttgaacagg 1301 gactgtttca aaaattccgc gtccgccgcc aaagccgcgt ccttcggatt 1351 gttcaggata tgcagttgca tgattttcct ctcattgccg taaatactgt 1401 aaatgtacct caaatgccgc atccgtgcca aaccgttcac actttaacca 1451 ctcatgtccc gaaatgccgt ctgaagttga acgccgcccg acggcggcgt 1501 tacaatcgcc cgcaactgtt tttttccgaa catcatcatg accgcgaccg 1551 aacacgacaa cgacgacgca ctcctgctgc ggtacagccg ccacatcctc 1601 ttggacgaaa tcggcatcga agggcagcag aagctttccg ccgcgcatat 1651 tttggtcgtc ggctgcggcg gattgggcgc cgccgcccct gccctatctc 1701 gccgcctcgg gggtcggcac gctga This corresponds to the amino acid sequence <SEQ ID 1394; ORF 509.ng>: g509.pep 1 MVAVCDERAV QRTLVAQFAQ QGGLFLLFVQ AVWFQACVL EKLGNHIGVF 51 ACVLAQVERH HVEAEHGHGT DEVCQTAFGK QAAAWDKGT LQFFQIIEKF 101 LGRSIRLEKA EFAAHAQTER ARFAHSARHN VGNGAAVRFF GAGDFFVRRE 151 GCQCHYVWD FDAADGKRQF AVKFVEFAAV KTEHGIGVAA EGKAQGFARN 201 KRIAVAVAAD PAADFKDIRN ADIGIGRLKV VFHLAVEFGQ GFKKAHREDG 251 HAWDFWDA EFVAARFAGL PQAQKHGVDF AAQPCQRVGI GAAFALRQQR 301 ADAAVEIQNG LALHFGRVRG QNGGNGRIVQ LPLHRFAVGF PRFEPADGFR 351 QAAFCCWAG IFVDLAAAFV VHVFGDIQNL GEQPAGKRQI VGLPFVQLRQ 401 YFFNQCRAW GSGQEFDRFD NQRRGFFVQE VEQGLFQKFR VRRQSRVLRI 451 VQDMQLHDFP LIAVNTVNVP QMPHPCQTVH TLTTHVPKCR LKLNAARRRR 501 YNRPQLFFSE HHHDRDRTRQ RRRTPAAVQP PHPLGRNRHR RAAEAFRRAY 551 FGRRLRRIGR RRPCPISPPR GSAR* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1395>: m509. seq 1 ATGGTCGCTG TATGTGATAA ACGGGCTGTA CAGAGGACGT TGATGGCTCA 51 ATTCGCGCAA CAGGGCGGTT TGTTTTTGCT CTTCGTTCAG GCGGTTGTAG 101 TCTTCCAAGC CTGCGTGTTG GAAAAGCTCG GCAACCACAT CGGCGTGTTT 151 GCCTGCGTGT TGGCGCAAGT CGAGCGGCAT CATGTGAAAG CCGAACACGG 201 ATACGGAACG GATGAGGTCT GCCAAACGGC CTTCGGCAAG CAGACGGCTG 251 CCGTTGTCGA TAAGGGAACG TTGCAATTTT TTCAAATCAT CCAGAAACTC 301 TTGTGCCGAA GCATAAGGCT CGAGAAAGCC GAATTTGCAG CCCATACCCA 351 AACCGAGCGC GCGCGCTTTG CCCATAGCGC GCGCCATAAT GTAGGCGATG 401 GCGCGGCGGT AGGGTTCTTC GGCGCGGGCG ATTTCTTCGT CGGGCGATTT 451 GTCGGACAAC GCCGTTACAT CGCCGTTGAC TTTGACGCGG CGGATGGAGA 501 GCGGCAGTTC GCGGTAGAGT TTGTCGAGTT CGCCGCGATA GAAGCGGAAC 551 ACGGCATCGG CGTGGCGGCG GAAGGCAAAG CGCAGGGTTT CGGCAGAAAC 601 AAACGGATTG CCGTCGCGGT CGCCGCCGAT CCAGCCGCCG ATTTTGAGGA 651 TGTCCGGAAC GCGGACGCCG GGATAGGCCG TCTGAAAGTC GTGTTCCATC 701 TTGCGGTAGA GCTTGGGCAG GGCTTCGAAA AAGCTCATCG GGAAGATGGA 751 CACGCCGTTG TTGATTTCGT CGTTGACGCT GAGTTTGTGG CGGCGCGTTT 801 CGCTGGTCTG CCACAAGCCC AGCAGGATAG TGTCGATTtC GCgGCGCAGC 851 CGTGCCAGCG CGTCGGCATT GGTGCAGCGT TCgCGTTGCG GCAACAGTGC 901 GCGGATGCGG CGGTTGAAGC TTAAGACGGT TTGGCGTTGC ACTTCGGTCG 951 GGTGCGCGGT CAAAACGGCG GTAACGGACG TATTGTCCAA CTGCCGCTGC 1001 ACCGATTTGC CGTCGGCTTT CCCCGCTTTG AGCCTGCGGA CGGTTTCCGT 1051 CAGGCTGCCT TCCGCGCCGC CGCGTCCGGC TTCTTCGTGG ATTTGGCGGC 1101 GGCGTTCGTG GTGCACGTCT TCGGCGATGT TCAAAATCTG GGCGAACAGG 1151 CCGCAGGCCA AGGTTAAATC GTGGGTTTGT TGTTCGTCCA ATTGCGGCAA 1201 TACTTTTTCA ATCAATGCCG CGCTGTCGTC GGAAGTGGAC AAGAGTTTGA 1251 CTGTTTCGAC AACCAACGGC GAGGCTTCTT CGTGCAGGAG GTTGAACAGG 1301 GATTGTTTCA GAAATTCCGC GTCCGCCGCC AAAGCCGCGT CCTTTGGATT 1351 GTTCAGAATA TGCAGTTGCA TGATTTTTCT CTCTCGTCTG CCGTAAATAT 1401 TGTAAATGTA CCCCAAATGC CGCATCCGTG CCAAACCGTT CACACTTTAA 1451 CCGCCCGTGT CCCGAAATGC CGTCTGAAGT TGAACGCCGC CCGACGGCAG 1501 CGTTACAATC GCCCGCAACT GTTTTtTTCC GAACATCATC ATGACCACGA 1551 CCGAACACGA CAACGACGAT GCATTCCTGC TGCGGTACAG CCGCCACATC 1601 CTCTTGGACG AAATCGGCAT CGAAGGGCAG CAGAAACTTT CCGCCGCGCA 1651 TATTTTGGTC GTCGGCTGCG GCGGTTTGGG TGCCGCCGCA CT.GCCCTAC 1701 CTTGCCGCTT CGGGTGTCGG CACGCTGA This corresponds to the amino acid sequence <SEQ ID 1396; ORF 509>: m509.pep 1 MVAVCDKRAV QRTLMAQFAQ QGGLFLLFVQ AVWFQACVL EKLGNHIGVF 51 ACVLAQVERH HVKAEHGYGT DEVCQTAFGK QTAAWDKGT LQFFQIIQKL 101 LCRSIRLEKA EFAAHTQTER ARFAHSARHN VGDGAAVGFF GAGDFFVGRF 151 VGQRRYIAVD FDAADGERQF AVEFVEFAAI EAEHGIGVAA EGKAQGFGRN 201 KRIAVAVAAD PAADFEDVRN ADAGIGRLKV VFHLAVELGQ GFEKAHREDG 251 HAWDFWDA EFVAARFAGL PQAQQDSVDF AAQPCQRVGI GAAFALRQQC 301 ADAAVEAXDG LALHFGRVRG QNGGNGRIVQ LPLHRFAVGF PRFEPADGFR 351 QAAFRAAASG FFVDLAAAFV VHVFGDVQNL GEQAAGQGXI VGLLFVQLRQ 401 YFFNQCRAW GSGQEFDCFD NQRRGFFVQE VEQGLFQKFR VRRQSRVLWI 451 VQNMQLHDFS LSSAVNIVNV PQMPHPCQTV HTLTARVPKC RLKLNAARRQ 501 RYNRPQLFFS EHHHDHDRTR QRRCIPAAVQ PPHPLGRNRH RRAAETFRRA 551 YFGRRLRRFG CRRTXPTLPL RVSAR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 509 shows 87.8% identity over a 575 aa overlap with a predicted ORF (ORF 509.ng) from N gonorrhoeae: m509/g509 10 20 30 40 50 60 m509. pep MVAVCDKRAVQRTLMAQFAQQGGLFLLFVQAVWFQACVLEKLGΝHIGVFACVLAQVERH MMM MMM MMMMMMMMMMMMMMMMMMMMMM g509 MVAVCDERAVQRTLVAQFAQQGGLFLLFVQAVWFQACVLEKLGΝHIGVFACVLAQVERH 10 20 30 40 50 60 70 80 90 100 110 120 IT1509 . pep HVKAEHGYGTDEVCQTAFGKQTAAWDKGTLQFFQI IQKLLCRSIRLEKAEFAAHTQTER MMMIMMMMMMMMMMMMMMMMM MMMMIMMMIM g509 HVEAEHGHGTDEVCQTAFGKQAAAWDKGTLQFFQIIEKFLGRSIRLEKAEFAAHAQTER 70 80 90 100 110 120 130 140 150 160 170 180 m509. pep ARFAHSARHΝVGDGAAVGFFGAGDFFVGRFVGQRRYIAVDFDAADGERQFAVEFVEFAAI M M M M M M M I M l l l l l l l l l I I = h M 1 1 1 I I 1 1 M I I I I M I I I I I = g509 ARFAHSARHΝVGΝGAAVRFFGAGDFFVRREGCQCHYVWDFDAADGKRQFAVKFVEFAAV 130 140 150 160 170 180 190 200 210 220 230 240 m509.pep EAEHGIGVAAEGKAQGFGRNKRIAVAVAADPAADFEDVRNADAGIGRLKWFHLAVELGQ = MMMMMMIMIMMMMMMMMMMMIM MMMMMMMMI g509 KTEHGIGVAAEGKAQGFARNKRIAVAVAADPAADFKDIRNADIGIGRLKWFHLAVEFGQ 190 200 210 220 230 240 250 260 270 280 290 300 m509. pep GFEKAHREDGHAWDFWDAEFVAARFAGLPQAQQDSVDFAAQPCQRVGIGAAFALRQQC MMMIMMMMMMMMMIMMMM : M II II I II 1111 II I II I II 11 g509 GFKKAHREDGHAWDFWDAEFVAARFAGLPQAQKHGVDFAAQPCQRVGIGAAFALRQQR 250 260 270 280 290 300 310 320 330 340 350 360 m509. pep ADAAVEAXDGLALHFGRVRGQNGGNGRIVQLPLHRFAVGFPRFEPADGFRQAAFRAAASG MMMMMMMMMMMMMMMMMMMMMMM - g509 ADAAVEIQNGLALHFGRVRGQNGGNGRIVQLPLHRFAVGFPRFEPADGFRQAAFCCWAG 310 320 330 340 350 360 370 380 390 400 410 420 π \ 509. pep FFVDLAAAFWHVFGDVQNLGEQAAGQGXIVGLLFVQLRQYFFNQCRAWGSGQEFDCFD MMMMMMMMMIMM lh llll 1111 II II I II II II II I II I II II g509 IFVDLAAAFWHVFGDIQNLGEQPAGKRQIVGLPFVQLRQYFFNQCRAWGSGQEFDRFD 370 380 390 400 410 420 430 440 450 460 470 480 m509. pep NQRRGFFVQEVEQGLFQKFRVRRQSRVLWIVQNMQLHDFSLSSAVNIVNVPQMPHPCQTV IMMIIIM I III g509 NQRRGFFVQEVEQGLFQKFRVRRQSRVLRIVQDMQLHDFPLI -AVNTVNVPQMPHPCQTV 430 440 450 460 470 490 500 510 520 530 540 m509.pep HTLTARVPKCRLKLNAARRQRYNRPQLFFSEHHHDHDRTRQRRCIPAAVQPPHPLGRNRH MlhMMMMMMMMMMMMMMMMMMM g509 HTLTTHVPKCRLKLNAARRRRYNRPQLFFSEHHHDRDRTRQRRRTPAAVQPPHPLGRNRH 480 490 500 510 520 530 550 560 570 m509. pep RRAAETFRRAYFGRRLRRFGCRRTCPTLPLRVSAR MMIMMMIMMMM ll ll I I III g509 RRAAEAFRRAYFGRRLRRIGRRRPCPISPPRGSAR 540 550 560 570 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1397>: a509.seq 1 ATGGTCGCTG TATGTGATGA ACGGACTGTA CAGTGGACGT TGATGGCTCA 51 ATTCGCGCAA CAGGGCGGCT TGTTTTTGCT CTTCGTTGAG GCTGTTGTAG 101 TCTTCCAAGC CTGCGTGTTG GAAAAGCTCG GCAACCACAT CGGCGTGTTT 151 GCCTGCGTGT TGGCGCAGGT CGAGCGGCAT CATGTGGAAG CCGAACACGG 201 ATACGGAACG GATGAGGTCT GCCAAACGGC CTTCGGCAAG CAGGCGGCTG 251 CCGTTGTCGA TAAGGGAATG TTGCAATTTT TTCAAATCAT CGAGAAATTC 301 TTGTGCCGAA GCATAAGGCT CGAGAAAGCC GAATTTGCAG CCCATACCCA 351 AACCGAGCGC GCGCGCTTTG CCCATAGCGC GCGCCA AAT GTAGGCAATG 401 GCGCGACGGT AGGGTTCTTC GGCGCGGGCG GTTTCTTCGT CGGGCGATTT 451 GTCGGACAAC GCCATCACAT CGCCGTTGAC TTTGACGCGG CGGATGGAGA 501 GCGGCAGTTC GCGGTAGAGT TTGTCGAGTT CGCCACGGTA AAAACGGAAC 551 ACGGCATCGG CGTGGCGGCG GAAGGCAAAA CGCAAGGTTT CGGCAGAAAC 601 GAACGGATTG CCGTCGCGGT CGCCGCCGAT CCAGCCGCCG ATTTTGAGGA 651 TGTCCGGAAC GCGGACATCG GGATAGGCCG TCTGAAAGTC GTGTTCCATC 701 TTGCGGTAGA GCTTGGGCAG GGCTTCAAAA AAGCTCATCG GAAAGATGGA 751 CACGCCGTTG TTGATTTCGT CGTTGACGCT GAGTTTGTGG CGGCGCGTTT 801 CGCTGGTCTG CCACAAGCCC AGCAGGATAG TGTCGATTTC GCGGCGCAGC 851 CGTGCCAGCG CGTCGGCATT GGTACAGCGT TCGCGTTGCG GCAGCAGCGC 901 GCGGATGCGG CGGTTGAAAT TCAAGACGGT CTGGCGTTGC ACTTCGGTCG 951 GGTGCGCGGT CAAAACGGCG GTAACGGACG TATTGTCCAA CTGCCGCTGC 1001 ACCGATTTGC CGTCGGCTTT CCCCGCTTTG AGCCTGCGGA CGGTTTCCGT 1051 CAGGCTGCCT TCCGCGCCGC CGCGTCCGGC TTCTTCGTGG ATTTGGCGGC 1101 GGCGTTCGTG GTGCACGTCT TCGGCGATGT TCAAAATCTG GGCGAACAGG 1151 CCGCAGGCCA AGGTTAAATC GTGGGTTTGT TGTTCGTCCA ATTGCGGCAA 1201 TACTTTTTCA ATCAATGCCG CGCTGTCGTC GGAAGTGGAC AAGAGTTTGA 1251 CCGTTTCGAC AACCAACGGC GAGGCTTCTT CGTGCAGGAG GTTGAACAGG 1301 GATTGTTTCA GAAATTCCGC GTCCGCCGCC AAAGCCGCGT CCTTTGGATT 1351 GTTCAGAATA TGCAGTTGCA TGATTTTTCT CTCATTGCCG TAAATACTGT 1401 AAATGTACCT CAAATGCCGC ATCCGTGCCA AACCGTTCAC ACTTTAACCG 1451 CCCGTGTCCC GAAATGCCGT CTGAAGTTGA ACGCCGCCCG ACGGCAGCGT 1501 TACAATCGCC CACAACTGTT TTT . TCCGAA CATCATCATG ACCACGACCG 1551 AACACGACAA CGACGATGCA TTCCTGCTGC GGTACAGCCG CCACATCCTC 1601 TTGGACGAAA TTGGCATCGA AGGGCAGCAG AAACTTTCCG CCGCGCATAT 1651 TTTGGTCGTC GGCTGCGGCG GTTTGGGTGC CGCCG.CCCT GCCCTATCTC 1701 GCCGCTTCCG GCATCGGCAC GCTGA This corresponds to the amino acid sequence <SEQ ID 1398; ORF 509. a>: a509.pep 1 MVAVCDERTV QWTLMAQFAQ QGGLFLLFVE AVVVFQACVL EKLGNHIGVF 51 ACVLAQVERH HVEAEHGYGT DEVCQTAFGK QAAAVVDKGM LQFFQIIEKF 101 LCRSIRLEKA EFAAHTQTER ARFAHSARHN VGNGATVGFF GAGGFFVGRF 151 VGQRHHIAVD FDAADGERQF AVEFVEFATV KTEHGIGVAA EGKTQGFGRN 201 ERIAVAVAAD PAADFEDVRN ADIGIGRLKV VFHLAVELGQ GFKKAHRKDG 251 HAWDFWDA EFVAARFAGL PQAQQDSVDF AAQPCQRVGI GTAFALRQQR 301 ADAAVEIQDG LALHFGRVRG QNGGNGRIVQ LPLHRFAVGF PRFEPADGFR 351 QAAFRAAASG FFVDLAAAFV VHVFGDVQNL GEQAAGQG*I VGLLFVQLRQ 401 YFFNQCRAW GSGQEFDRFD NQRRGFFVQE VEQGLFQKFR VRRQSRVLWI 451 VQNMQLHDFS LIAVNTVNVP QMPHPCQTVH TLTARVPKCR LKLNAARRQR 501 YNRPQLFXSE HHHDHDRTRQ RRCIPAAVQP PHPLGRNWHR RAAETFRRAY 551 FGRRLRRFGC RXPCPISPLP ASAR* m509/a509 93.0% identity in 575 aa overlap 10 20 30 40 50 60 m509 . pep MVAVCDKRAVQRTLMAQFAQQGGLFLLFVQAVWFQACVLEKLGNHIGVFACVLAQVERH I I I I I I : I : I I I I I I I I I I I I I I I I I I h I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a509 MVAVCDERTVQWTLMAQFAQQGGLFLLFVEAWVFQACVLEKLGNHIGVFACVLAQVERH 10 20 30 40 50 60 70 80 90 100 110 120 m509. pep HVKAEHGYGTDEVCQTAFGKQTAAVVDKGTLQFFQIIQKLLCRSIRLEKAEFAAHTQTER I I : I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I : I : I I I I I I I I I I I I I I I I I II I a509 HVEAEHGYGTDEVCQTAFGKQAAAWDKGMLQFFQIIEKFLCRSIRLEKAEFAAHTQTER 70 80 90 100 110 120 130 140 150 160 170 180 m509 . pep ARFAHSARHNVGDGAAVGFFGAGDFFVGRFVGQRRYIAVDFDAADGERQFAVEFVEFAAI I I I I I I I I I I I I : I I : I I I I I I I I I I I I I I I I I : : I I I I I I I I I I I I I I I I I I I I I I : : a509 ARFAHSARHNVGNGATVGFFGAGGFFVGRFVGQRHHIAVDFDAADGERQFAVEFVEFATV 130 140 150 160 170 180 190 200 210 220 230 240 m509 . pep EAEHGIGVAAEGKAQGFGRNKRIAVAVAADPAADFEDVRNADAGIGRLKWFHLAVELGQ : : I I I I I I I I I I I : I I I I I I : I I I I I I I I I I I I I I I I I I I I I l l l l l l l l l l l l l l l l l a509 KTEHGIGVAAEGKTQGFGRNERIAVAVAADPAADFEDVRNADIGIGRLKWFHLAVELGQ 190 200 210 220 230 240 250 260 270 280 290 300 m509 . pep GFEKAHREDGHAWDFWDAEFVAARFAGLPQAQQDSVDFAAQPCQRVGIGAAFALRQQC I I : I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I | : I M I I I I a509 GFKKAHRKDGHAWDFVVDAEFVAARFAGLPQAQQDSVDFAAQPCQRVGIGTAFALRQQR 250 260 270 280 290 300 310 320 330 340 350 360 m509. pep ADAAVEAXDGLALHFGRVRGQNGGNGRIVQLPLHRFAVGFPRFEPADGFRQAAFRAAASG llllll I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I a509 ADAAVEIQDGLALHFGRVRGQNGGNGRIVQLPLHRFAVGFPRFEPADGFRQAAFRAAASG 310 320 330 340 350 360 370 380 390 400 410 420 m509.pep FFVDLAAAFVVHVFGDVQNLGEQAAGQGXIVGLLFVQLRQYFFNQCRAWGSGQEFDCFD I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II II a509 FFVDLAAAFWHVFGDVQNLGEQAAGQGXIVGLLFVQLRQYFFNQCRAWGSGQEFDRFD 370 380 390 400 410 420 430 440 450 460 470 480 m509.pep NQRRGFFVQEVEQGLFQKFRVRRQSRVLWIVQNMQLHDFSLSSAVNIVNVPQMPHPCQTV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I III a509 NQRRGFFVQEVEQGLFQKFRVRRQSRVLWIVQNMQLHDFSLI-AVNTVNVPQMPHPCQTV 430 440 450 460 470 490 500 510 520 530 540 m509. pep HTLTARVPKCRLKLNAARRQRYNRPQLFFSEHHHDHDRTRQRRCIPAAVQPPHPLGRNRH I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a509 HTLTARVPKCRLKLNAARRQRYNRPQLFXSEHHHDHDRTRQRRCIPAAVQPPHPLGRNWH 480 490 500 510 520 530 550 560 570 m509.pep RRAAETFRRAYFGRRLRRFGCRRTXPTLPLRVSARX I I I I I I I I I I I I I I I I I I I I I I I II I I I I a509 RRAAETFRRAYFGRRLRRFGCRXPCPISPLPASARX 540 550 560 570 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1399>: g510. seq 1 atgccttcgc ggacaccgca gggaaaaagg ggttattcct gccccaagcg 51 ggatagtgcc ttttggcagg cgttgtccat atcggttatt ttacgcgcaa 101 aatcgccgat tgccaaatcg ccgccgttca gggaggtttt caataggtcg 151 tggacgacgt tgagcgcggc cataatgacg attttttcgc tgtccgcgac 201 gcggccgcct tcgcggatgg cttcggcttt gccgttgagc attccgactg 251 cctgcaacag tgtgtctttt tcttctgccg gcgtgttgac agtcagccgg 301 ggcgtgcatg acttcgatgt agacttgttc gatgttcatc ctttaatcct 351 tattgctgcg tttcctgccg ttgggggagg cgcgctgcca gtgcgctga This corresponds to the amino acid sequence <SEQ ID 1400; ORF 510.ng>: g510.pep 1 MPSRTPQGKR GYSCPKRDSA FWQALSISVI LRAKSPIAKS PPFREVFΝRS 51 WTTLSAAIMT IFSLSATRPP SRMASALPLS IPTACΝSVSF SSAGVLTVSR 101 GVHDFDVDLF DVHPLILIAA FPAVGGGALP VR* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1401>: m510. seq 1 ATGCCTTCGC GGACACCGCA GGGnAAAAGG GGTTATTCCT GCGCCAAGCG 51 GGATAGTGCT TTTTGGCAGG CGTTGTCCAT ATCGGCTATT TTACGCGCAA 101 AATCGCCGAT TGCCAAATCG CCGCCGTTCA GGGAGGTTTT CAACAGGTCG 151 TGGACGACGT TGAGCGCGGC CATAATGACG ATTTTTTCGC TGTCCGCGAC 201 GCGTCCGCCT TCGCGGATGG CTTCGGCTTT GCCGTTGAGC ATTCCGACTG 251 CCTGCAACAG TGTGTCTTTT TCTTCTGCCG GCGTGTTGAC GGTCAGCCGG 301 GGCGTGCA G ACTTCsAtGT GGACTTGTTC GATGTTCATC CTTTAATCCT 351 TATTGCTGCG TTTCCTGCCA TTGGGGGAGG CGCGCTGCCA GTGCGCTGA This corresponds to the amino acid sequence <SEQ ID 1402; ORF 510>: m510.pep 1 MPSRTPQGKR GYSCAKRDSA FWQALSISAI LRAKSPIAKS PPFREVFΝRS 51 WTTLSAAIMT IFSLSATRPP SRMASALPLS IPTACΝSVSF SSAGVLTVSR 101 GVXDFXVDLF DVHPLILIAA FPAIGGGALP VR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 510 shows 96.2% identity over a 132 aa overlap with a predicted ORF (ORF 510.ng) from N gonorrhoeae: m510/g510 10 20 30 40 50 60 m510.pep MPSRTPQGKRGYSCAKRDSAFWQALSISAILRAKSPIAKSPPFREVFΝRSWTTLSAAIMT llllllllllllll I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g510 MPSRTPQGKRGYSCPKRDSAFWQALSISVILRAKSPIAKSPPFREVFΝRSWTTLSAAIMT 10 20 30 40 50 60 70 80 90 100 110 120 m510.pep IFSLSATRPPSRMASALPLSIPTACΝSVSFSSAGVLTVSRGVXDFXVDLFDVHPLILIAA I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I II llllllllllllll g510 IFSLSATRPPSRMASALPLSIPTACΝSVSFSSAGVLTVSRGVHDFDVDLFDVHPLILIAA 70 80 90 100 110 120 130 m510.pep FPAIGGGALPVRX I I I : I I I I I I I I I g510 FPAVGGGALPVRX 130 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1403>: a510. seq 1 ATGCCTTCGC GGACACCGCA GGGAAAAAGG GGTTATTCCT GCGCCAAGCG 51 GGATAGTGCT TTTTGGCAGG CGTTGTCCAT ATCGGCTATT TTACGCGCAA 101 AATCGCCGAT TGCCAAATCG CCGCCGTTCA GGGAGGTTTT CAACAGGTCG 151 TGGACGACGT TGAGCGCGGC CATAATGACG ATTTTTTCGC TGTCCGCGAC 201 GCGTCCGCCT TCGCGGATGG CTTCGGCTTT GCCGTTGAGC ATTCCGACTG 251 CCTGCAACAG TGTGTCTTTT TCTTCTGCCG GCGTGTTGAC GGTCAGCCGG 301 G.CGTGCATG ACTTCGATGT GGACTTGTTC GATGTTCATC CTTTAATCCT 351 TATTGCTGCG TTTCCTGCCG TTGGGGGAGG CGCGCTGCCA GTGCGCTGA This corresponds to the amino acid sequence <SEQ ID 1404; ORF 510.a>: a510.pep 1 MPSRTPQGKR GYSCAKRDSA FWQALSISAI LRAKSPIAKS PPFREVFΝRS 51 WTTLSAAIMT IFSLSATRPP SRMASALPLS IPTACΝSVSF SSAGVLTVSR 101 XVHDFDVDLF DVHPLILIAA FPAVGGGALP VR* m510/a510 97.0% identity in 132 aa overlap 10 20 30 40 50 60 m510.pep MPSRTPQGKRGYSCAKRDSAFWQALSISAILRAKSPIAKSPPFREVFΝRSWTTLSAAIMT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a510 MPSRTPQGKRGYSCAKRDSAFWQALSISAILRAKSPIAKSPPFREVFΝRSWTTLSAAIMT 10 20 30 40 50 60 70 80 90 100 110 120 m510. pep IFSLSATRPPSRMASALPLSIPTACΝSVSFSSAGVLTVSRGVXDFXVDLFDVHPLILIAA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II llllllllllllll a510 IFSLSATRPPSRMASALPLSIPTACΝSVSFSSAGVLTVSRXVHDFDVDLFDVHPLILIAA 70 80 90 100 110 120 130 m510.pep FPAIGGGALPVRX I I I : I I I I I I I I I a510 FPAVGGGALPVRX 130 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 1405>: g512.seq 1 atgaaagtgc ttgttttagg tgcgggtgtt gccggcgtat cctccgtgtg 51 gtatctggca gaggccggac atgaagtaac ggtcatcgac cgcaccgagg 101 gtgtggcgat ggaaaccagt tttgccaatg caggccagct ttcttacggc 151 tataccacgc cttgggctgc acccggtatt ccgaccaaag cactgaaacg 201 gctgtttaaa agccatccgc ctttactgtt ccgccctgac ggcggcctgt 251 atcaaatcga atggctgtgg cggatgctgc aaaactgcac ggcaacgcgc 301 tatcaaatca ataaagagcg catggtcagg atttccgaat acagccgtga 351 aatgttccgc cgttttgaag cgcaaaccga catgaatttt gagggacgca 401 aaaaagggac gttgcagatt ttccgccaaa ccgaagaagt cgaagcggca 451 aaacaagaca ttgccgtttt ggaacgctac ggcgtgccgt accgccgtct 501 gaagcccgaa gaatgcgcag aattcgagcc tgcgctggca cgcgttaccg 551 ccaaaattgt cggcggtctg cacctgcctg cggatgcgac cggcgactgc 601 cgcctcttca ccgaaaacct gtacaaattg tgtcaagaga agggggtacg 651 gttctacttc aaccaaacca tcagccgcat cgaccacaac gggctgcgca 701 tcaaagccgt tgaaacgaaa cagggcggtt tgaaacagat gccgttgtct 751 gcgcgctcgg ctgcttcagc aggactgtgt tggcgcagtt ggatctcaat 801 ctgcccattt atcccgtcaa aggctattcc ttga This corresponds to the amino acid sequence <SEQ ID 1406; ORF 512.ng>: g512.pep 1 MKVLVLGAGV AGVSSVWYLA EAGHEVTVID RTEGVAMETS FANAGQLSYG 51 YTTPWAAPGI PTKALKRLFK SHPPLLFRPD GGLYQIEWLW RMLQNCTATR 101 YQINKERMVR ISEYSREMFR RFEAQTDMNF EGRKKGTLQI FRQTEEVEAA 151 KQDIAVLERY GVPYRRLKPE ECAEFEPALA RVTAKIVGGL HLPADATGDC 201 RLFTENLYKL CQEKGVRFYF NQTISRIDHN GLRIKAVETK QGGLKQMPLS 251 ARSAASAGLC WRSWISICPF IPSKAIP* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1407>: m512.seq (partial) 1 ..GTTTTGGAAC GCTACGGCGT GCCGTACCGC CGTCTGAAAC CCGAAGAATG 51 TGCAGAATTT GAGCCTGCGC TGGCACGCGT TACCGCCAAA ATTGCCGGCG 101 GCCTGCACCT GCCTGCAGAT GCGACCGGCG ACTggCGCCT CTTCACTGAA 151 AACCTATACA AATTGTGTCA GGAAAAGGGC GTACGGTTTC ATTTCAACCA 201 AAACATCAGC CGCATCGACC ACAACGGGCT GCGCATCAAA ACCGTTGAAA 251 CCAAACAGGG CGGTTTGAAG CAGATGCCGT TGTCTGCGCG CTCGGTTGCT 301 TCAGCAGGAC GGTTTTGGCG CAGTTGGATC TCAATCTGCC CATTTATCCC 351 GTCAAAGGCT ATTCCTTGA This corresponds to the amino acid sequence <SEQ ID 1408; ORF 512>: m512.pep (partial) 1 ..VLERYGVPYR RLKPEECAEF EPALARVTAK IAGGLHLPAD ATGDWRLFTE 51 ΝLYKLCQEKG VRFHFΝQΝIS RIDHΝGLRIK TVETKQGGLK QMPLSARSVA 101 SAGRFWRSWI SICPFIPSKA IP* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 512 shows 93.4% identity over a 122 aa overlap with a predicted ORF (ORF 512.ng) from N gonorrhoeae: m512/g512 10 20 30 m512.pep VLERYGVPYRRLKPEECAEFEPALARVTAK MMMMMMMMMMMMMMM g512 TDMΝFEGRKKGTLQIFRQTEEVEAAKQDIAVLERYGVPYRRLKPEECAEFEPALARVTAK 130 140 150 160 170 180 40 50 60 70 80 90 m512. pep IAGGLHLPADATGDWRLFTEΝLYKLCQEKGVRFHFΝQΝISRIDHΝGLRIKTVETKQGGLK hi 11111111111 MMMMMMMMMMMMMIMMMMMMMMM g512 IVGGLHLPADATGDCRLFTEΝLYKLC Q EKGVRFYFΝQTISRIDHΝGLRIKAVETKQGGLK 190 200 210 220 230 240 100 110 120 m512.pep QMPLSARSVASAGRFWRSWISICPFIPSKAIP MMMMMIM MMMIMMMMM g512 QMPLSARSAASAGLCWRSWISICPFIPSKAIP 250 260 270 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 1409>: a512. seq 1 ATGAAAGTGC TTGTTTTAGG TGCTGGTGTT GCCGGCGTAT CTTCCGCGTG 51 GTATCTGGCA GAGGCAGGAC ATGAAGTAAC GGTCATCGAC CGCGCCGAGG 101 GCGTGGCGAT GGAAACCAGT TTTGCCAACG CAGGCCAGCT TTCTTACGGC 151 TATACCACGC CTTGGGCTGC ACCCGGTATT CCGACCAAAG CACTGAAATG 201 GCTGTTTAAA AGCCATCCGC CTTTGCTGTT TCGCCCCGAC GGCAGCCTGT 251 ATCAAATCGA ATGGCTGTGG CAGATGCTGC AACACTGCAC GGCAGCGCGC 301 TATCAAATCA ATAAAGAGCG CATGGTCAGG ATGTCCGAAT ACAGCCGTGA 351 AATGTTCCGC CGTTTTGAAG CGCAAACCGG CATGAATTTT GAGGGACGCA 401 AAAAAGGGAC GTTGCAGATT TTCCGCCAAA CCAAAGAAGT CGAAGCGGCA 451 AAACAAGACA TTGCCGTTTT GGAACGCTAC GGCGTGCCGT ACCGCCGTCT 501 GAAGCCCGAA GAATGCGCAG AATTCGAGCC TGCGCTGGCA CGCGTTACCG 551 CCAAAATTGC CGGCGGCCTG CACCTGCCCG CAGACGCGAC CGGCGACTGC 601 CGCCTCTTCA CTGAAAACCT GTACAAATTG TGTCAGGAAA AGGGCGTACG 651 GTTTCATTTC AACCAAACCA TCAGCCGCAT CGACCACAAC GGGCTGCGCA 701 TCAAAACCGT TGAAACGAAA CAGGGCGGTT TGAAGCAGAT GCCGTTGTCT 751 GCGCGCTCGG CTGCTTCAGC AGGACGGTTT TGGCGCAAGT GGATCTCAAT 801 CTGCCGATTT ATCCCGTCAA AGGCTATTCC TTGA This corresponds to the amino acid sequence <SEQ ID 1410; ORF 512.a>: a512.pep 1 MKVLVLGAGV AGVSSAWYLA EAGHEVTVID RAEGVAMETS FANAGQLSYG 51 YTTPWAAPGI PTKALKWLFK SHPPLLFRPD GSLYQIEWLW QMLQHCTAAR 101 YQINKERMVR MSEYSREMFR RFEAQTGMNF EGRKKGTLQI FRQTKEVEAA 151 KQDIAVLERY GVPYRRLKPE ECAEFEPALA RVTAKIAGGL HLPADATGDC 201 RLFTENLYKL CQEKGVRFHF NQTISRIDHN GLRIKTVETK QGGLKQMPLS 251 ARSAASAGRF WRKWISICRF IPSKAIP* m512/a512 95.9% identity in 122 aa overlap 10 20 30 m512.pep VLERYGVPYRRLKPEECAEFEPALARVTAK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a512 TGMNFEGRKKGTLQIFRQTKEVEAAKQDIAVLERYGVPYRRLKPEECAEFEPALARVTAK 130 140 150 160 170 180 40 50 60 70 80 90 m512. pep IAGGLHLPADATGDWRLFTENLYKLCQEKGVRFHFNQNISRIDHNGLRIKTVETKQGGLK llllllllllllll I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I a512 IAGGLHLPADATGDCRLFTENLYKLCQEKGVRFHFNQTISRIDHNGLRIKTVETKQGGLK 190 200 210 220 230 240 100 110 120 m512.pep QMPLSARSVASAGRFWRSWISICPFIPSKAIPX I I I I I I I I : I I I I I I I I : I I I I I a512 QMPLSARSAASAGRFWRKWISICRFIPSKAIPX 250 260 270 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1411>: g513.seq 1 ATGGGTTCCG CGCCGAACGC CGCCGCCGCC GCCGAAGTGA AACACCCTGT 51 TTCGCAAGGT ATGATTCAAA TGCTGGGCGT GTTTGTCGAT ACCATCATCG 101 TTTGTTCTTG CACCGCCTTC ATCATCTTGA TTTACCAACA GCCTTATGGC 151 GATTTGAGCG GTGCGGCGCT GAcgcAGGCG GCGATTGTCA GCCAAGTGGG 201 GCAATGGGGC GCGGGTTTCC TCGCCGTCAT CCTGTTTATG TTTGCCTTTT 251 CCACCGTTAT CGGCAACTAT GCCTATGCCG AGTCCAACGT CCAATTCATC 301 AAAAGCCATT GGCTGATTAC CGCCGTTTTC CGTATGCTGG TTTTGGCGTG 351 GGTCTATTTC GGCGCGGTTG CCAATGTGCC TTTGGTCTGG GATATGGCGG 401 ATATGGCGAT GGGCATCATG GCGTGGATCA ACCTCGTCGC CATCCTGCTG 451 CTCTCGCCat TGGCGTTTAT GCTGCTGCGC GATTACACCG CCAAGCTGAA 501 AATGGGCAAA GACCCCGAGT TCAAACTTTc cgAACATCCG GGCCTGAAAC 551 GCCGCATCAA ATCCGATGTT TGGTAA This corresponds to the amino acid sequence <SEQ ID 1412; ORF 513.ng>: g513 . pep 1 MGSAPNAAAA AEVKHPVSQG MIQMLGVFVD TIIVCSCTAF IILIYQQPYG 51 DLSGAALTQA AIVSQVGQWG AGFLAVILFM FAFSTVIGNY AYAESNVQFI 101 KSHWLITAVF RMLVLAWVYF GAVANVPLVW DMADMAMGIM AWINLVAILL 151 LSPLAFMLLR DYTAKLKMGK DPEFKLSEHP GLKRRIKSDV W* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1413>: m513.seq 1 ATGGGTTCCG CGCCGAACGC CGCCGCCGCC GCCGAAGTGA AACACCCTGT 51 TTCGCAAGGT ATGATTCAAA TGCTGGGCGT GTTTGTCGAT ACCATCATCG 101 TTTGTTCTTG CACCGCCTTC ATCATCTTGA TTTACCAACA GCCTTATGGC 151 GATTTGAGCG GTGCGGCGCT GAcgcAGGCG GCGATTGTCA GCCAAGTGGG 201 GCAATGGGGC GCGGGTTTCC TCGCCGTCAT CCTGTTTATG TTTGCCTTTT 251 CCACCGTTAT CGGCAACTAT GCCTATGCCG AGTCCAACGT CCAATTCATC 301 AAAAGCCATT GGCTGATTAC CGCCGTTTTC CGTATGCTGG TTTTGGCGTG 351 GGTCTATTTC GGCGCGGTTG CCAATGTGCC TTTGGTCTGG GATATGGCGG 401 ATATGGCGAT GGGCATCATG GCGTGGATCA ACCTCGTCGC CATCCTGCTG 451 CTCTCGCCat TGGCGTTTAT GCTGCTGCGC GATTACACCG CCAAGCTGAA 501 AATGGGCAAA GACCCCGAGT TCAAACTTTc cgAACATCCG GGCCTGAAAC 551 GCCGCATCAA ATCCGATGTT TGGTAA This corresponds to the amino acid sequence <SEQ ID 1414; ORF 513>: m513.pep 1 MGSAPNAAAA AEVKHPVSQG MIQMLGVFVD TIIVCSCTAF IILIYQQPYG 51 DLSGAALTQA AIVSQVGQWG AGFLAVILFM FAFSTVIGNY AYAESNVQFI 101 KSHWLITAVF RMLVLAWVYF GAVANVPLVW DMADMAMGIM AWINLVAILL 151 LSPLAFMLLR DYTAKLKMGK DPEFKLSEHP GLKRRIKSDV W* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 513 shows 99.5% identity over a 191 aa overlap with a predicted ORF (ORF 513.ng) from N gonorrhoeae: m513/g513 10 20 30 40 50 60 m513.pep MGSAPΝAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYGDLSGAALTQA MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g513 MGSAPΝAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYGDLSGAALTQA 10 20 30 40 50 60 70 80 90 100 110 120 m513.pep AIVSQVGQWGAGFLAVILFMFAFSTVIGΝYAYAESΝVQFIKSHWLITAVFRMLVLAWVYF g513 AIVSQVGQWGAGFLAVILFMFAFSTVIGΝYAYAESΝVQFIKSHWLITAVFRMLVLAWVYF 70 80 90 100 110 120 130 140 150 160 170 180 m513.pep GAVAΝVPLVWDMADMAMGIMAWIΝLVAILLLSPLAFMXLRDYTAKLKMGKDPEFKLSEHP g513 GAVAΝVPLVWDMADMAMGIMAWIΝLVAILLLSPLAFMLLRDYTAKLKMGKDPEFKLSEHP 130 140 150 160 170 180 190 m513. pep GLKRRIKSDVW g513 GLKRRIKSDVW 190 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1415>: a513 . seq 1 ATGAACGAGA ACTTTACCGA ATGGCTGCAC GGCTGGGTCG GCGCCATCAA 51 CGATCCGATG TGGTCATACT TGGTTTATNT GCTTTTGGGT ACGGGGCTTT 101 TCTTCACCGT AACCACGGGC TTTGTCCAAT TCCGCCTGTT CGGGCGCAGC 151 ATCAAAGAAA TGCTCGGCGG CCGCAAACAG GGGGACGACC CTCACGGCAT 201 CACGCCGTTT CAGGCATTTG TAACCGGCCT TGCCAGCCGC GTGGGCGTGG 251 GCAATATCGC GGGCGTGGCC ATCGCCATCA AAGTCGGCGG ACCGGGCGCG 301 GTGTTTTGGA TGTGGGTAAC CGCCTTAATC GGTATGAGTT CGGCGTTTGT 351 CGAATCTTCG CTGGCGCAGC TCTTTAAAGT CCGCGACTAC GACAACCACC 401 ATTTCCGGGG CGGCCCTGCC TACTACATCA CTCAAGGGCT GGGGCAGAAA 451 TGGCTGGGCG TGTTGTTCGC CCTGAGCCTG ATTTTCTGTT TCGGCTTTGT 501 GTTTGAAGCG GTTCAGACCA ATACCATTGC CGATACCGTC AAAGCGGCGT 551 GGGGTTGGGA GCCTCATTAT GTCGGCGTCG CCCTGGTGAT TTTAACCGCG 601 CCGATTATCT TCGGCGGCAT CAGGCGCATA TCTAAAGCGG CGGAAATCGT 651 CGTCCCCCTG ATGGCGGTTT TGTACCTCTT TATCGCGCTT TTCATCATTT 701 TGACCAATAT TCCGATGATT CCGGACGTGT TCGGTCAGAT TTTTTCGGGC 751 GCGTTCAAAT TCGACGCGGC AGCAGGCGGC TTACTCGGCG GTCTGATTTC 801 GCAAACGATG ATGATGGGCA TCAAACGCGG CCTGTATTCC AACGAGGCGG 851 GTATGGGTTC CGCGCCGAAC GCCGCCGCCG CCGCCGAAGT GAAACACCCT 901 GTTTCGCAAG GTATGATTCA AATGCTGGGC GTGTTTGTCG ATACCATCAT 951 CGTTTGTTCT TGCACCGCCT TCATCATCTT GATTTACCAA CAGCCTTACG 1001 GCGATTTGAG CGGTGCGGCG CTGACGCAGG CGGCGATTGT CAGCCAAGTG 1051 GGGCAATGGG GCGCGGGCTT CCTCGCCGTC ATCCTGTTTA TGTTTGCCTT 1101 TTCCACCGTT ATCGGCAACT ATGCCTATGC CGAGTCCAAC GTCCAATTCA 1151 TCAAAAGCCA TTGGCTGATT ACCGCCGTTT TCCGTATGCT GGTTTTGGCG 1201 TGGGTCTATT TCGGCGCGGT TGCCAATGTG CCTTTGGTCT GGGATATGGC 1251 GGATATGGCG ATGGGCATTA TGGCGTGGAT CAACCTTGTC GCCATCCTGC 1301 TGCTCTCGCC CTTGGCGTTT ATGCTGCTGC GCGATTACAC CGCCAAGCTG 1351 AAAATGGGCA AAGACCCCGA GTTCAAACTT TCCGAACATC CGGGCCTGAA 1401 ACGCCGTATC AAATCCGACG TTTGGTAA This corresponds to the amino acid sequence <SEQ ID 1416; ORF 513. a>: a513. pep 1 MNENFTEWLH GWVGAINDPM WSYLVYXLLG TGLFFTVTTG FVQFRLFGRS 51 IKEMLGGRKQ GDDPHGITPF QAFVTGLASR VGVGNIAGVA IAIKVGGPGA 101 VFWMWVTALI GMSSAFVESS LAQLFKVRDY DNHHFRGGPA YYITQGLGQK 151 WLGVLFALSL IFCFGFVFEA VQTNTIADTV KAAWGWEPHY VGVALVILTA 201 PIIFGGIRRI SKAAEIVVPL MAVLYLFIAL FIILTNIPMI PDVFGQIFSG 251 AFKFDAAAGG LLGGLISQTM MMGIKRGLYS NEAGMGSAPN AAAAAEVKHP 301 VSQGMIQMLG VFVDTIIVCS CTAFIILIYQ QPYGDLSGAA LTQAAIVSQV 351 GQWGAGFLAV ILFMFAFSTV IGNYAYAESN VQFIKSHWLI TAVFRMLVLA 401 WVYFGAVANV PLVWDMADMA MGIMAWINLV AILLLSPLAF MLLRDYTAKL 451 KMGKDPEFKL SEHPGLKRRI KSDVW* m513/a513 100.0% identity in 191 aa overlap 10 20 30 m513 . pep MGSAPNAAAAAEVKHPVSQGMIQMLGVFVD I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a513 DAAAGGLLGGLISQTMMMGIKRGLYΞNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVD 260 270 280 290 300 310 40 50 60 70 80 90 m513.pep TIIVCSCTAFIILIYQQPYGDLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNY I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a513 TIIVCSCTAFIILIYQQPYGDLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNY 320 330 340 350 360 370 100 110 120 130 140 150 m513. pep AYAESNVQFIKSHWLITAVFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILL I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a513 AYAESNV Q FIKSHWLITAVFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILL 380 390 400 410 420 430 160 170 180 190 m513. pep LSPLAFMLLRDYTAKLKMGKDPEFKLSEHPGLKRRIKSDVWX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a513 LSPLAFMLLRDYTAKLKMGKDPEFKLSEHPGLKRRIKSDVWX 440 450 460 470 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1417>: g5l5.seq 1 atggttcaaa tacaggttgt gcgcgccgcc ggcgttgccc gtggtctgca 51 ttccgagttt gcgcgcgctg taactgccga ggaaatagcc ttcgacaatg 101 ccgttttgaa tcacgaagcg cggcgcggtg gcaacacctt ccgcatcaaa 151 atagctgctg cggaaagagc gggggatgtg cggttcttcg cgcaggttga 201 ggaaatcggg caggactttt ttgccgatgc tgtcgatcag gaaactgctt 251 tggcggtaga gcgcgccgcc ggagagtgtg ccgacgaggt gtccgatcag 301 cccgcccgaa acggtggtat cgaagaggac ggggtagctg cctgtcggga 351 tgctgcggct gccgagtcgg cgcaaagtgc ggcgggcggc ggtttgaccg 401 atggtttcgg ggctgtccat atccggatgg cggcaggcgg aatcgtacca 451 gtagtcgcgc tgcattccgt tttcgtcggc ggcgacgacg ctgcaggaaa 501 tgctgtggtg cgtgctttgc cggtgtgcgg caaaaccgtg ggtgttgccg 551 taaacgtatt ggtactgtcc ggtttgcacc gccgcgcctt cggagttttc 601 gatgcggctg tccgtgtcca acgctgcctg ttcgcattgt tttgccaagc 651 cgacggcggc ttccgtatcc aaatcccatt cgtggtaaag gtcggggtcg 701 ccgatgtgtt gcgccatcaa ctcggggtcg gcaagtccgg cgcaaccgtc 751 ttcggcggtg tggcgggcga tgtcggcggc ggcgcggacg gtgtcgcgca 801 gggcttgttc ggagaagtcg gcggtgccgg cgcggccttt gcgtttgccg 851 acgtaaacgg taatgtccag cgatttgtcc tgctggaact cgatttgttc 901 gatttcgccc aagcgcacgc tgacgctttg tccgagcgat tcgctgaagt 951 cggcttcggc ggcggtcgcg cccgctgctt ttgccaagtc gagcgtgcgg 1001 cggcagaggt cgaggagttc ggaagcggtg tggttgaaca gcataacaat 1051 ctttcttggt ggagcgttgt ggcattttaa This corresponds to the amino acid sequence <SEQ ID 1418; ORF 515.ng>: g515.pep 1 MVQIQWRAA GVARGLHSEF ARAVTAEEIA FDΝAVLΝHEA RRGGΝTFRIK 51 IAAAERAGDV RFFAQVEEIG QDFFADAVDQ ETALAVERAA GECADEVSDQ 101 PARΝGGIEED GVAACRDAAA AESAQSAAGG GLTDGFGAVH IRMAAGGIVP 151 WALHSVFVG GDDAAGΝAW RALPVCGKTV GVAVΝVLVLS GLHRRAFGVF 201 DAAVRVQRCL FALFCQADGG FRIQIPFWK VGVADVLRHQ LGVGKSGATV 251 FGGVAGDVGG GADGVAQGLF GEVGGAGAAF AFADVΝGΝVQ RFVLLELDLF 301 DFAQAHADAL SERFAEVGFG GGRARCFCQV ERAAAEVEEF GSGWEQHΝΝ 351 LSWWSWAF* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1419>: m515.seq (partial) 1 .. GGAAAGAGCG GGGGATGTGC GTTCTTCGCG CAGGTTGAGG AAATCGGGCA 51 GGACTTTTCT GCCGATGCTG TCGATCAGGA AACTGCTTTG GCGGTAGAGC 101 GCGCCGCCGG AGAGTGCGCC GACGAGGTGT CCGATAAGAC CGCCCGAAAC 151 GGTGGTATCG AAGAGGACGG GGTAGCTGCC TGTCGGGATG CTGCGGCTGC 201 CGAGTCGGCG CAAAGTGCGG CGGGCGGCGG TTTGACCGAT GGTTTCGGGG 251 CTGTCCATAT CCGGATGGCG GCAGGCGGAA TCGTACCAGT AGTCGCGCTG 301 CATGCCGTTT TCGTCGGCGG CAACGACGCT GCAGGAAATG CTGTGGTGCG 351 TGCCTTGCCG GTGTGCGGCA AAACCGTGGG TGTTGCCGTA AACGTATTGG 401 TAATGGCCGG TTTGCACCGC CGCGCCTTCG GAGTTTTCGA TGCGCTCATC 451 CTCGTTCAGG GCGGCTTGTT CGCATTGTTT TGCCAAGCCG ACGGCGGCTk 501 CCGTATCCAA ATCCCATTCG TGGTAAAGGT CGGGGTCGCC GATGTGTTTT 551 GCCATCAGAC AGGCATCGGC AAGTCCGGCG CAACCGTCTT CGGCGGTGTG 601 GCGGGCGATG TCGATGGCGG CTTTGACGGT GTCTTGCAGG GCTTTTTCGG 651 AGAAGTCGGC AGTACTGGCG CGGCCTTTGC GTTTGCCGAC GTAAACGGTA 701 ATGTCCAGCG ACTTGTCCTG CTGGAACTCG ATTTGTTsGA TTTsGCCCAG 751 CCGCACGCTG ACGCTTTGTC CCAATGATTC GCTGAAATCG GCTTCGGCGG 801 CGGTTGCGCC CGTCGCTTTT GCCAAGTCGA GCOTGCGGCG GCAGAGGTCG 851 AGGAGTTCGG AAGCGGTGTG GTTgAACAGC ATAGAAATCT TTCTTGATGA 901 TGCTTTGCGG CATTTTAA This corresponds to the amino acid sequence <SEQ ID 1420; ORF 515>: m515 . pep (partial ) 1 ..GKSGGCAFFA QVEEIGQDFS ADAVDQETAL AVERAAGECA DEVSDKTARN 51 GGIEEDGVAA CRDAAAAESA QSAAGGGLTD GFGAVHIRMA AGGIVPWAL 101 HAVFVGGNDA AGNAWRALP VCGKTVGVAV NVLVMAGLHR RAFGVFDALI 151 LVQGGLFALF CQADGGXRIQ IPFWKVGVA DVFCHQTGIG KSGATVFGGV 201 AGDVDGGFDG VLQGFFGEVG STGAAFAFAD VNGNVQRLVL LELDLXDXAQ 251 PHADALSQXF AEIGFGGGCA RRFCQVERAA AEVEEFGSGV VEQHRNLSXX 301 CFAAF* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 515 shows 85.9% identity over a 304 aa overlap with a predicted ORF (ORF 515.ng) from N gonorrhoeae: m515/g515 10 20 30 m515. pep GKSGGCAFFAQVEEIGQDFSADAVDQETALA ■■ ■ A lll g515 AEEIAFDΝAVLΝHEARRGGΝTFRIKIAAAERAGDVRFFAQVEEIGQDFFADAVDQETALA 30 40 50 60 70 80 40 50 60 70 80 90 m515. pep VERAAGECADEVSDKTARΝGGIEEDGVAACRDAAAAESAQSAAGGGLTDGFGAVHIRMAA 11111111111111= 11 ! 11 ! 111111111111111111 M 11111 I 111111111 ! 11 g515 VERAAGECADEVSDQPARΝGGIEEDGVAACRDAAAAESAQSAAGGGLTDGFGAVHIRMAA 90 100 110 120 130 140 100 110 120 130 140 150 m515. pep GGI VPWALHAVFVGGΝDAAGΝAWRALPVCGKTVGVAVΝVLVMAGLHRRAFGVFDALI L MMMMMMMMMMMMMMMIMMMMMIhMMMIMMM : g515 GGIVPWALHSVFVGGDDAAGΝAWRALPVCGKTVGVAVΝVLVLSGLHRRAFGVFDAAVR 150 160 170 180 190 200 160 170 180 190 200 210 m515. pep VQGGLFALFCQADGGXRIQIPFWKVGVADVFCHQTGIGKSGATVFGGVAGDVDGGFDGV II M II IMMMMMMMM II III g515 VQRCLFALFCQADGGFRIQI PFWKVGVADVLRHQLGVGKΞGATVFGGVAGDVGGGADGV 210 220 230 240 250 260 220 230 240 250 260 270 m515.pep LQGFFGEVGSTGAAFAFADVΝGΝVQRLVLLELDLXDXAQPHADALSQXFAEIGFGGGCAR MMMIhMMMMMMMMMMMM I II lllllh IIIMIIM II g515 AQGLFGEVGGAGAAFAFADVΝGΝVQRFVLLELDLFDFAQAHADALSERFAEVGFGGGRAR 270 280 290 300 310 320 280 290 300 m515.pep RFCQVERAAAEVEEFGSGWEQHRΝLSXXCFAAF IMM Ml I I i I III Ml g515 CFCQVERAAAEVEEFGSGWEQHΝΝLSWWSWAF 330 340 350 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1421>: a515. seq 1 ATGGTTCAAA TAAAGGTTGT GCGCGCCGCC GGCGTTGCCC GTGGTCTGCA 51 TTCCGAGTTT GCGCGCGCTG TAACTGCTGA GGAAATAGCC TTCGACAATG 101 CCGTTTTGAA TCACGAAGCG CGGTGCGGTG GCAACGCCTT CCGCATCAAA 151 ATAGCTGCTG CGGAAAGAGC GGGGGATGTG CGGTTCTTCG CGCAGGTTGA 201 GGAAATCGGG CAGGACTTTT TTGCCGATGC TGTCGATCAG GAAACTGCTT 251 TGGCGGTAGA GCGCTCCGCC GGAGAGTGCG CCGACGAGGT GTCCGATAAG 301 ACCGCCCGAA ACGGTGGTAT CGAAGAGGAC GGGGTAGTTG CCTGTCGGGA 351 TGCTGCGGCT GCCGAGTCGG CGCAAAGTGC GGCGGGCGGC GGTTTGACCG 401 ATGGTTTCGG GGCTGTCCAT ATCCGGATGG CGGCAGGCGG AATCGTACCA 451 GTAGTCGCGC TGCATGCCGT TTTCGTCGGC GGCAACGACG CTGCAGGAAA 501 TGCTGTGGTG CGTGCTTTGC CGGTGTGCGG CAAAACCGTA GGTGTTGCCG 551 TAAACGTATT GGTAATGGCC GGTTTGCACC GCCGCGCCTT CGGAGTTTTC 601 GATGCGCTCA TCCTCGTTCA GGGCGGCTTG TTCGCATTGT TTTGCCAAGC 651 CGACGGCGGC TTCCGTATCC AAATCCCATT CGTGGTAAAG GTCGGGGTCG 701 CCGATGTGTT GCGCCATCAA CTCGGGGTCG GCAAGTCCGG CGCAACCGTC 751 TTCGGCGGTG TGGCGGGCGA TGTCNNNNGC GGCGCGGACG GTGTCGCGCA 801 GGGCTTGTTC GGAGAAATCG GCGGTGCCGG CGCGGCCTTT GCGTTTGCCG 851 ACGTAAACGG TAATGTCCAG CGACTTGTCC TGCTGAAACT CGATTTGTTC 901 GATTTCGCCC AGCCGCACGC TGACGCTTTG TCCCAATGAT TCGCTGAAAT 951 CGGCTTCGGC GGCGGTTGCG CCCGTCGCTT TTGCCAAGTC GAGCGTGCGG 1001 CGGCAGAGGT CGAGGAGTTC GGAAGCGGTG TGGTTGAACA GCATAGAAAT 1051 CTTTCTTGAT GATGCTTTGC GGCATTTTAA This corresponds to the amino acid sequence <SEQ ID 1422; ORF 515.a>: a515.pep 1 MVQIKWRAA GVARGLHSEF ARAVTAEEIA FDNAVLNHEA RCGGNAFRIK 51 IAAAERAGDV RFFAQVEEIG QDFFADAVDQ ETALAVERSA GECADEVSDK 101 TARNGGIEED GWACRDAAA AESAQΞAAGG GLTDGFGAVH IRMAAGGIVP 151 WALHAVFVG GNDAAGNAVV RALPVCGKTV GVAVNVLVMA GLHRRAFGVF 201 DALILVQGGL FALFCQADGG FRIQIPFVVK VGVADVLRHQ LGVGKSGATV 251 FGGVAGDVXX GADGVAQGLF GEIGGAGAAF AFADVNGNVQ RLVLLKLDLF 301 DFAQPHADAL SQ*FAEIGFG GGCARRFCQV ERAAAEVEEF GSGWEQHRN 351 LS**CFAAF* m515/a515 92.1% identity in 304 aa overlap 10 20 30 m515.pep GKSGGCAFFAQVEEIGQDFSADAVDQETALA : : I I I I I I I I I I I I I I I I I I I I I I I I a515 AEEIAFDNAVLNHEARCGGNAFRIKIAAAERAGDVRFFAQVEEIGQDFFADAVDQETALA 30 40 50 60 70 80 40 50 60 70 80 90 m515. pep VERAAGECADEVSDKTARNGGIEEDGVAACRDAAAAESAQSAAGGGLTDGFGAVHIRMAA I I I : I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a515 VERSAGECADEVSDKTARNGGIEEDGVVACRDAAAAESAQSAAGGGLTDGFGAVHIRMAA 90 100 110 120 130 140 100 110 120 130 140 150 m515. pep GGIVPVVALHAVFVGGNDAAGNAVVRALPVCGKTVGVAVNVLVMAGLHRRAFGVFDALIL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I a515 GGIVPWALHAVFVGGNDAAGNAVVRALPVCGKTVGVAVNVLVMAGLHRRAFGVFDALIL 150 160 170 180 190 200 160 170 180 190 200 210 m515. pep VQGGLFALFCQADGGXRIQIPFWKVGVADVFCHQTGIGKSGATVFGGVAGDVDGGFDGV lllllllllllllll lllllllllllllll II I : I I I I I I I I I I I I I I I I III a515 VQGGLFALFCQADGGFRIQIPFWKVGVADVLRHQLGVGKSGATVFGGVAGDVXXGADGV 210 220 230 240 250 260 220 230 240 250 260 270 m515. pep LQGFFGEVGSTGAAFAFADVNGNVQRLVLLELDLXDXAQPHADALSQXFAEIGFGGGCAR I I : I I I: I :: I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I a515 AQGLFGEIGGAGAAFAFADVNGNVQRLVLLKLDLFDFAQPHADALSQXFAEIGFGGGCAR 270 280 290 300 310 320 280 290 300 m515. pep RFCQVERAAAEVEEFGSGWEQHRNLSXXCFAAFX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a515 RFCQVERAAAEVEEFGSGVVEQHRNLSXXCFAAFX 330 340 350 360 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1423>: g515-l.seq 1 ATGGTTCAAA TACAGGTTGT GCGCGCCGCC GGCGTTGCCC GTGGTCTGCA 51 TTCCGAGTTT GCGCGCGCTG TAACTGCCGA GGAAATAGCC TTCGACAATG 101 CCGTTTTGAA TCACGAAGCG CGGCGCGGTG GCAACACCTT CCGCATCAAA 151 ATAGCTGCTG CGGAAAGAGC GGGGGATGTG CGGTTCTTCG CGCAGGTTGA 201 GGAAATCGGG CAGGACTTTT TTGCCGATGC TGTCGATCAG GAAACTGCTT 251 TGGCGGTAGA GCGCGCCGCC GGAGAGTGTG CCGACGAGGT GTCCGATCAG 301 CCCGCCCGAA ACGGTGGTAT CGAAGAGGAC GGGGTAGCTG CCTGTCGGGA 351 TGCTGCGGCT GCCGAGTCGG CGCAAAGTGC GGCGGGCGGC GGTTTGACCG 401 ATGGTTTCGG GGCTGTCCAT ATCCGGATGG CGGCAGGCGG AATCGTACCA 451 GTAGTCGCGC TGCATTCCGT TTTCGTCGGC GGCAACGACG CTGCAGGAAA 501 TGCTGTGGTG CGTGCTTTGC CGGTGTGCGG CAAAACCGTG GGTGTTGCCG 551 TAAACGTATT GGTAGTGTCC GGTTTGCACC GCCGCGCCTT CGGAGTTTTC 601 GATGCGGCTG TCCGTGTCCA ACGCTGCCTG TTCGCATTGT TTTGCCAAGC 651 CGACGGCGGC TTCCGTATCC AAATCCCATT CGTGGTAAAG GTCGGGGTCG 701 CCGATGTGTT GCGCCATCAA CTCGGGGTCG GCAAGTCCGG CGCAACCGTC 751 TTCGGCGGTG TGGCGGGCGA TGTCGGCGGC GGCGCGGACG GTGTCGCGCA 801 GGGCTTGTTC GGAGAAGTCG GCGGTGCCGG CGCGGCCTTT GCGTTTGCCG 851 ACGTAAACGG TAATGTCCAG CGATTTGTCC TGCTGGAACT CGATTTGTTC 901 GATTTCGCCC AAGCGCACGC TGACGCTTTG TCCGAGCGAT TCGCTGAAGT 951 CGGCTTCGGC GGCGGTCGCG CCCGCTGCTT TTGCCAAGTC GAGCGTGCGG 1001 CGGCAGAGGT CGAGGAGTTC GGAAGCGGTG TGGTTGAACA GCATAACAAT 1051 CTTTCTTGGT GGAGCGTTGT GGCATTTTAA This corresponds to the amino acid sequence <SEQ ID 1424; ORF 515-l.ng>: g515-l.pep 1 MVQIQVVRAA GVARGLHSEF ARAVTAEEIA FDNAVLNHEA RRGGNTFRIK 51 IAAAERAGDV RFFAQVEEIG QDFFADAVDQ ETALAVERAA GECADEVSDQ 101 PARNGGIEED GVAACRDAAA AESAQSAAGG GLTDGFGAVH IRMAAGGIVP 151 VVALHSVFVG GNDAAGNAVV RALPVCGKTV GVAVNVLVVS GLHRRAFGVF 201 DAAVRVQRCL FALFCQADGG FRIQIPFVVK VGVADVLRHQ LGVGKSGATV 251 FGGVAGDVGG GADGVAQGLF GEVGGAGAAF AFADVNGNVQ RFVLLELDLF 301 DFAQAHADAL SERFAEVGFG GGRARCFCQV ERAAAEVEEF GSGVVEQHNN 351 LSWWSVVAF* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1425>: m515-l.seq 1 ATGGTTCAAA TACAGGTTGT GCGCGCCGCC GGCGTTGCCC GTGGTCTGCA 51 TACCGAGTTT GCGCGCGCTG TAACTGCTGA GGAAATAGCC TTCGACAATG 101 CCGTTTTGAA TCACGAAGCG CGGTGCGGTG GCAACGCCTT CCGCATCAAA 151 ATAGCTGCTG CGGAAAGAGC GGGGGATGTG CGGTTCTTCG CGCAGGTTGA 201 GGAAATCGGG CAGGACTTTT TTGCCGATGC TGTCGATCAG GAAACTGCTT 251 TGGCGGTAGA GCGCGCCGCC GGAGAGTGCG CCGACGAGGT GTCCGATAAG 301 ACCGCCCGAA ACGGTGGTAT CGAAGAGGAC GGGGTAGCTG CCTGTCGGGA 351 TGCTGCGGCT GCCGAGTCGG CGCAAAGTGC GGCGGGCGGC GGTTTGACCG 401 ATGGTTTCGG GGCTGTCCAT ATCCGGATGG CGGCAGGCGG AATCGTACCA 451 GTAGTCGCGC TGCATGCCGT TTTCGTCGGC GGCAACGACG CTGCAGGAAA 501 TGCTGTGGTG CGTGCCTTGC CGGTGTGCGG CAAAACCGTG GGTGTTGCCG 551 TAAACGTATT GGTAATGGCC GGTTTGCACC GCCGCGCCTT CGGAGTTTTC 601 GATGCGCTCA TCCTCGTTCA GGGCGGCTTG TTCGCATTGT TTTGCCAAGC 651 CGACGGCGGC TTCCGTATCC AAATCCCATT CGTGGTAAAG GTCGGGGTCG 701 CCGATGTGTT TTGCCATCAG ACAGGCATCG GCAAGTCCGG CGCAACCGTC 751 TTCGGCGGTG TGGCGGGCGA TGTCGATGGC GGCTTTGACG GTGTCTTGCA 801 GGGCTTTTTC GGAGAAGTCG GCAGTACTGG CGCGGCCTTT GCGTTTGCCG 851 ACGTAAACGG TAATGTCCAG CGACTTGTCC TGCTGGAACT CGATTTGTTC 901 GATTTCGCCC AGCCGCACGC TGACGCTTTG TCCCAATGA This corresponds to the amino acid sequence <SEQ ID 1426; ORF 515-1>: m515-l .pep 1 MVQIQVVRAA GVARGLHTEF ARAVTAEEIA FDNAVLNHEA RCGGNAFRIK 51 IAAAERAGDV RFFAQVEEIG QDFFADAVDQ ETALAVERAA GECADEVSDK 101 TARNGGIEED GVAACRDAAA AESAQΞAAGG GLTDGFGAVH IRMAAGGIVP 151 WALHAVFVG GNDAAGNAVV RALPVCGKTV GVAVNVLVMA GLHRRAFGVF 201 DALILVQGGL FALFCQADGG FRIQIPFVVK VGVADVFCHQ TGIGKSGATV 251 FGGVAGDVDG GFDGVLQGFF GEVGSTGAAF AFADVNGNVQ RLVLLELDLF 301 DFAQPHADAL SQ* m515-l/g515-l 91.7% identity in 312 aa overlap 10 20 30 40 50 60 g515-l.pep MVQIQVVRAAGVARGLHSEFARAVTAEEIAFDNAVLNHEARRGGNTFRIKIAAAERAGDV I I I I II I I I I I I I I I I hi I I I I I I I I I I I I I I I I I I I I I I I | | :| I I I I I I I I I I I I I m515-1 MVQIQVVRAAGVARGLHTEFARAVTAEEIAFDNAVLNHEARCGGNAFRIKIAAAERAGDV 10 20 30 40 50 60 70 80 90 100 110 120 g515-l.pep RFFAQVEEIGQDFFADAVDQETALAVERAAGECADEVSDQPARNGGIEEDGVAACRDAAA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I h I I I I I I I I I I I I I I I I I I I m515-l RFFAQVEEIGQDFFADAVDQETALAVERAAGECADEVSDKTARNGGIEEDGVAACRDAAA 70 80 90 100 110 120 130 140 150 160 170 180 g515-l.pep AESAQSAAGGGLTDGFGAVHIRMAAGGIVPVVALHSVFVGGNDAAGNAVVRALPVCGKTV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I hi I I I I I I I I I I I I II I I I I I I I I I m515-l AESAQSAAGGGLTDGFGAVHIRMAAGGIVPVVALHAVFVGGNDAAGNAVVRALPVCGKTV 130 140 150 160 170 180 190 200 210 220 230 240 g515-1. pep GVAVNVLVVSGLHRRAFGVFDAAVRVQRCLFALFCQADGGFRIQIPFVVKVGVADVLRHQ I I I I I I I h:| I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I : II m515-l GVAVNVLVMAGLHRRAFGVFDALILVQGGLFALFCQADGGFRIQIPFVVKVGVADVFCHQ 190 200 210 220 230 240 250 260 270 280 290 300 g515-1. pep LGVGKSGATVFGGVAGDVGGGADGVAQGLFGEVGGAGAAFAFADVNGNVQRFVLLELDLF I : I I I I I I I I I I I I I I I II III I I: I I I I h:| I I I I I I I I I I I I I h I I I I I I I I m515-1 TGIGKSGATVFGGVAGDVDGGFDGVLQGFFGEVGSTGAAFAFADVNGNVQRLVLLELDLF 250 260 270 280 290 300 310 320 330 340 350 360 g515-l . pep DFAQAHADALSERFAEVGFGGGRARCFCQVERAAAEVEEFGSGVVEQHNNLSWWSVVAFX llll lllllh m515-l DFAQPHADALSQX 310 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1427>: a515-l.seq 1 ATGGTTCAAA TAAAGGTTGT GCGCGCCGCC GGCGTTGCCC GTGGTCTGCA 51 TTCCGAGTTT GCGCGCGCTG TAACTGCTGA GGAAATAGCC TTCGACAATG 101 CCGTTTTGAA TCACGAAGCG CGGTGCGGTG GCAACGCCTT CCGCATCAAA 151 ATAGCTGCTG CGGAAAGAGC GGGGGATGTG CGGTTCTTCG CGCAGGTTGA 201 GGAAATCGGG CAGGACTTTT TTGCCGATGC TGTCGATCAG GAAACTGCTT 251 TGGCGGTAGA GCGCTCCGCC GGAGAGTGCG CCGACGAGGT GTCCGATAAG 301 ACCGCCCGAA ACGGTGGTAT CGAAGAGGAC GGGGTAGTTG CCTGTCGGGA 351 TGCTGCGGCT GCCGAGTCGG CGCAAAGTGC GGCGGGCGGC GGTTTGACCG 401 ATGGTTTCGG GGCTGTCCAT ATCCGGATGG CGGCAGGCGG AATCGTACCA 451 GTAGTCGCGC TGCATGCCGT TTTCGTCGGC GGCAACGACG CTGCAGGAAA 501 TGCTGTGGTG CGTGCTTTGC CGGTGTGCGG CAAAACCGTA GGTGTTGCCG 551 TAAACGTATT GGTAATGGCC GGTTTGCACC GCCGCGCCTT CGGAGTTTTC 601 GATGCGCTCA TCCTCGTTCA GGGCGGCTTG TTCGCATTGT TTTGCCAAGC 651 CGACGGCGGC TTCCGTATCC AAATCCCATT CGTGGTAAAG GTCGGGGTCG 701 CCGATGTGTT GCGCCATCAA CTCGGGGTCG GCAAGTCCGG CGCAACCGTC 751 TTCGGCGGTG TGGCGGGCGA TGTCGGCGGC GGCGCGGACG GTGTCGCGCA 801 GGGCTTGTTC GGAGAAATCG GCGGTGCCGG CGCGGCCTTT GCGTTTGCCG 851 ACGTAAACGG TAATGTCCAG CGACTTGTCC TGCTGAAACT CGATTTGTTC 901 GATTTCGCCC AGCCGCACGC TGACGCTTTG TCCCAATGA This corresponds to the amino acid sequence <SEQ ID 1428; ORF 515-1. a>: a515-l.pep 1 MVQIKVVRAA GVARGLHSEF ARAVTAEEIA FDNAVLNHEA RCGGNAFRIK 51 IAAAERAGDV RFFAQVEEIG QDFFADAVDQ ETALAVERSA GECADEVSDK 101 TARNGGIEED GVVACRDAAA AEΞAQΞAAGG GLTDGFGAVH IRMAAGGIVP 151 WALHAVFVG GNDAAGNAVV RALPVCGKTV GVAVNVLVMA GLHRRAFGVF 201 DALILVQGGL FALFCQADGG FRIQIPFVVK VGVADVLRHQ LGVGKSGATV 251 FGGVAGDVGG GADGVAQGLF GEIGGAGAAF AFADVNGNVQ RLVLLKLDLF 301 DFAQPHADAL SQ* m515-l/a515-l 94.9% identity in 312 aa overlap 10 20 30 40 50 60 a515-l . pep MVQIKWRAAGVARGLHSEFARAVTAEEIAFDNAVLNHEARCGGNAFRIKIAAAERAGDV I I I I : I I I I I I I I I I I hi I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I m515-l MVQIQVVRAAGVARGLHTEFARAVTAEEIAFDNAVLNHEARCGGNAFRIKIAAAERAGDV 10 20 30 40 50 60 70 80 90 100 110 120 a515-l.pep RFFAQVEEIGQDFFADAVDQETALAVERSAGECADEVSDKTARNGGIEEDGVVACRDAAA I I I I I I I I I I I I I I I I I I I I I I I I I I I h I I I I I I II I I I I I I I I I I I I I I I : II I I I I I m515- l RFFAQVEEIGQDFFADAVDQETALAVERAAGECADEVSDKTARNGGIEEDGVAACRDAAA 70 80 90 100 110 120 130 140 150 160 170 180 a515-1 . pep AESAQSAAGGGLTDGFGAVHIRMAAGGIVPWALHAVFVGGNDAAGNAWRALPVCGKTV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m515 - 1 AESAQSAAGGGLTDGFGAVHI RMAAGGI VPVVALHAVFVGGNDAAGNAVVRALPVCGKTV 130 140 150 160 170 180 190 200 210 220 230 240 a515-1 . pep GVAVNVLVMAGLHRRAFGVFDALILVQGGLFALFCQADGGFRIQI PFVVKVGVADVLRHQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I m515-1 GVAVNVLVMAGLHRRAFGVFDALI VQGGLFALFCQADGGFRIQIPFWKVGVADVFCHQ 190 200 210 220 230 240 250 260 270 280 290 300 a515-l.pep LGVGKSGATVFGGVAGDVGGGADGVAQGLFGEIGGAGAAFAFADVNGNVQRLVLLKLDLF h I I I I I I I I I I I I I I I II III I I : I I I : I ::| I I I I I I I I I I I I I I I I I I : I I I I II1515-1 TGIGKSGATVFGGVAGDVDGGFDGVLQGFFGEVGΞTGAAFAFADVNGNVQRLVLLELDLF 250 260 270 280 290 300 310 a515-l.pep DFAQPHADALSQX lllllllllllll m515-l DFAQPHADALSQX 310 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1429>: g516. seq 1 atgttgttcc gtaaaacgac cgccgccgtt ttggcggcaa ccttgatact 51 gaacggctgt acgatgatgt tgcgggggat gaacaacccg gtcagccaaa 101 caatcacccg caaacacgtt gacaaagacc aaatccgcgc cttcggtgtg 151 gttgccgaag acaatgccca attggaaaag ggcagcctgg tgatgatggg 201 cgggaaatac tggttcgccg tcaatcccga agattcggcg aagctgacgg 251 gccttttgaa ggccgggttg gacaagccct tccaaatagt tgaggatacc 301 ccgagctatg cccgccacca agccctgccg gtcaaattcg aagcgcccgg 351 cagccagaat ttcagtaccg gaggtctttg cctgcgctat gataccggca 401 gacctgacga catcgccaag ctgaaacagc ttgagtttaa agcggtcaaa 451 ctcgacaatc ggaccattta cacgcgctgc gtatccgcca aaggcaaata 501 ctacgccacg ccgcaaaaac tgaacgccga ttatcatttt gagcaaagtg 551 tgcccgccga tatttattat acggttactg aaaaacatac cgacaaatcc 601 aagctgtttg gaaatatctt atatacgccc cccttgttga tattggatgc 651 ggcggccgcg gtgctggtct tgcctatggc tctgattgca gccgcgaatt 701 cctcagacaa atga This corresponds to the amino acid sequence <SEQ ID 1430; ORF 516.ng>: g516.pep 1 MLFRKTTAAV LAATLILΝGC TMMLRGMΝΝP VSQTITRKHV DKDQIRAFGV 51 VAEDΝAQLEK GSLVMMGGKY WFAVΝPEDSA KLTGLLKAGL DKPFQIVEDT 101 PSYARHQALP VKFEAPGSQΝ FSTGGLCLRY DTGRPDDIAK LKQLEFKAVK 151 LDΝRTIYTRC VSAKGKYYAT PQKLΝADYHF EQSVPADIYY TVTEKHTDKS 201 KLFGΝILYTP PLLILDAAAA VLVLPMALIA AAΝSSDK* The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 1431>: m516. seq 1 ATGTTGTTCC GTAAAACGAC CGCCGCCGTT TTGGCGGCAA CCTTGATGCT 51 GAACGGCTGT ACGTTGATGT TGTGGGGAAT GAACAACCCG GTCAGCGAAA 101 CAATCACCCG CAAACACGTT GACAAAGACC AAATCCGCGC CTTCGGTGTG 151 GTTGCCGAAG ACAATGCCCA ATTGGAAAAG GGCAGCCTGG TGATGATGGG 201 CGGAAAATAC TGGTTCGTCG TCAATCCCGA AGATTCGGCG AAGCTGACGG 251 GCATTTTGAA GGCAGGGCTG GACAAACCCT TCCAAATAGT TGAGGATACC 301 CCGAGCTATG CTCGCCACCA AGCCCTGCCG GTCAAACTCG AATCGCCTGG 351 CAGCCAGAAT TTCAGTACCG AAGGCCTTTG CCTGCGCTAC GATACCGACA 401 AGCCTGCCGA CATCGCCAAG CTGAAACAGC TCGGGTTTGA AGCGGTCAAA 451 CTCGACAATC GGACCATTTA CACGCGCTGC GTATCCGCCA AAGGCAAATA 501 CTACGCCACA CCGCAAAAAC TGAACGCCGA TTACCATTTT GAGCAAAGTG 551 TGCCTGCCGA TATTTATTAC ACGGTTACTG AAGAACATAC CGACAAATCC 601 AAGCTGTTTG CAAATATCTT ATATACGCCC CCCTTTTTGA TACTGGATGC 651 GGCGGGCGCG GTACTGGCCT TGCCTGCGGC GGCTCTGGGT GCGGTCGTGG 701 ATGCCGCCCG CAAATGA This corresponds to the amino acid sequence <SEQ ID 1432; ORF 516>: m516.pep 1 MLFRKTTAAV LAATLMLNGC TLMLWGMNNP VSETITRKHV DKDQIRAFGV 51 VAEDNAQLEK GSLVMMGGKY WFWNPEDSA KLTGILKAGL DKPFQIVEDT 101 PSYARHQALP VKLESPGSQN FSTEGLCLRY DTDKPADIAK LKQLGFEAVK 151 LDNRTIYTRC VSAKGKYYAT PQKLNADYHF EQSVPADIYY TVTEEHTDKS 201 KLFANILYTP PFLILDAAGA VLALPAAALG AWDAARK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 516 shows 90.0% identity over a 231 aa overlap with a predicted ORF (ORF 516.ng) from N gonorrhoeae: 10 20 30 40 50 60 m516. pep MLFRKTTAAVLAATLMLΝGCTLMLWGMΝΝPVSETITRKHVDKDQIRAFGWAEDΝAQLEK 111111111 M 1111 = 11111 H I I II 111 I : I II 1111111111 II 11111111 M 11 g516 MLFRKTTAAVLAATLILΝGCTMMLRGMΝΝPVSQTITRKHVDKDQIRAFGWAEDΝAQLEK 10 20 30 40 50 60 70 80 90 100 110 120 m516.pep GSLVMMGGKYWFWΝPEDSAKLTGILKAGLDKPFQIVEDTPSYARHQALPVKLESPGSQΝ MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g516 GSLVMMGGKYWFAVΝPEDSAKLTGLLKAGLDKPFQIVEDTPSYARHQALPVKFEAPGSQΝ 70 80 90 100 110 120 130 140 150 160 170 180 m516. pep FSTEGLCLRYDTDKPADIAKLKQLGFEAVKLDΝRTIYTRCVSAKGKYYATPQKLΝADYHF I I I l l l l l l l l M I I I M M M M M M M M M M M M M M M M M g516 FSTGGLCLRYDTGRPDDIAKLKQLEFKAVKLDΝRTIYTRCVSAKGKYYATPQKLΝADYHF 130 140 150 160 170 180 190 200 210 220 230 239 m516.pep EQSVPADIYYTVTEEHTDKSKLFAΝILYTPPFLILDAAGAVLALPAAALGAWDAARK I M M M M M I M M M M M I M M M M M I M M M M M I I = = 1 = g516 EQSVPADIYYTVTEKHTDKSKLFGΝILYTPPLLILDAAAAVLVLPMALIAAAΝSSDK 190 200 210 220 230 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1433>: a516. seq 1 ATGTTGTTCC GTAAAACGAC CGCCGCCGTT TTGGCGGCAA CCTTGATGTT 51 GAACGGCTGT ACGGTAATGA TGTGGGGTAT GAACAGCCCG TTCAGCGAAA 101 CGACCGCCCG CAAACACGTT GACAAGGACC AAATCCGCGC CTTCGGTGTG 151 GTTGCCGAAG ACAATGCCCA ATTGGAAAAG GGCAGCCTGG TGATGATGGG 201 CGGGAAATAC TGGTTCGTCG TCAATCCTGA AGATTCGGCG AAGCTGACGG 251 GCATTTTGAA GGCCGGGTTG GACAAGCAGT TTCAAATGGT TGAGCCCAAC 301 CCGCGCTTTG CCTACCAAGC CCTGCCGGTC AAACTCGAAT CGCCCGCCAG 351 CCAGAATTTC AGTACCGAAG GCCTTTGCCT GCGCTACGAT ACCGACAGAC 401 CTGCCGACAT CGCCAAGCTG AAACAGCTTG AGTTTGAAGC GGTCGAACTC 451 GACAATCGGA CCATTTACAC GCGCTGCGTC TCCGCCAAAG GCAAATACTA 501 CGCCACACCG CAAAAACTGA ACGCCGATTA TCATTTTGAG CAAAGTGTGC 551 CTGCCGATAT TTATTACACG GTTACGAAAA AACATACCGA CAAATCCAAG 601 TTGTTTGAAA ATATTGCATA TACGCCCACC ACGTTGATAC TGGATGCGGT 651 GGGCGCGGTG CTGGCCTTGC CTGTCGCGGC GTTGATTGCA GCCACGAATT 701 CCTCAGACAA ATGA This corresponds to the amino acid sequence <SEQ ID 1434; ORF 516.a>: a516. pep 1 MLFRKTTAAV LAATLMLNGC TVMMWGMNSP FSETTARKHV DKDQIRAFGV 51 VAEDNAQLEK GSLVMMGGKY WFVVNPEDSA KLTGILKAGL DKQFQMVEPN 101 PRFAYQALPV KLESPASQNF STEGLCLRYD TDRPADIAKL KQLEFEAVEL 151 DNRTIYTRCV SAKGKYYATP QKLNADYHFE QSVPADIYYT VTKKHTDKSK 201 LFENIAYTPT TLILDAVGAV LALPVAALIA ATNSSDK* m516/a516 86.1% identity in 238 aa overlap 10 20 30 40 50 60 m516. pep MLFRKTTAAVLAATLMLNGCTLMLWGMNNPVSETITRKHVDKDQIRAFGWAEDNAQLEK I I I I I I I I I I I I I I I I I I I I I : I : I I I I : I III : I I I I I I I I I I I I I I I I I I I I I I I I a516 MLFRKTTAAVLAATLMLNGCTVMMWGMNSPFSETTARKHVDKDQIRAFGWAEDNAQLEK 10 20 30 40 50 60 70 80 90 100 110 120 m516.pep GSLVMMGGKYWFWNPEDSAKLTGILKAGLDKPFQIVEDTPSYARHQALPVKLESPGSQN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I 11:11 : I : I : I I I I I I I I I I : I I I a516 GSLVMMGGKYWFWNPEDSAKLTGILKAGLDKQFQMVEPNPRFA-YQALPVKLESPASQN 70 80 90 100 110 130 140 150 160 170 180 m516.pep FSTEGLCLRYDTDKPADIAKLKQLGFEAVKLDNRTIYTRCVSAKGKYYATPQKLNADYHF I I I I I I I I I I I I I : II I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a516 FSTEGLCLRYDTDRPADIAKLKQLEFEAVELDNRTIYTRCVSAKGKYYATPQKLNADYHF 120 130 140 150 160 170 190 200 210 220 230 239 m516.pep EQSVPADIYYTVTEEHTDKSKLFANILYTPPFLILDAAGAVLALPAAALGAWDAARKX a516 EQSVPADIYYTVTKKHTDKSKLFENIAYTPTTLILDAVGAVLALPVAALIAATNSSDKX 180 190 200 210 220 230 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1435>: g517.seq 1 atgcatcggg tttcagacgg cattggagtg tcagtcgtgt tctgccgatt 51 cgtaggcttc gacgattttt tgcaccagag gatgccggac aacgtcttcg 101 ccggtgaagg tatggaaata cagtcctgcc acgccgtgca gtttctcacg 151 tgcgtctttc aatcccgatt tgatgttttt gggcaggtcg atttggctgg 201 tgtcgccggt aatgacggct ttcgcgccga agccgatgcg ggtcaggaac 251 attttcattt gttcgggcgt ggtgttttgc gcttcgtcga ggatgatgta 301 tgcgccgttg agcgtcctgc cgcgcatata ggcgagcggg gcgatttcaa 351 tcaggccttt ttcaatcagc ttggttacac ggtcaaagcc catcaggtca 401 tagagggcat cataaagcgg acggaggtag gggtcgactt tttgggtcag 451 gtctccgggc aggaagccca gtttctcacc ggcttcgacg gcaggccgaa 501 ctaa This corresponds to the amino acid sequence <SEQ ID 1436; ORF 517.ng>: g517.pep 1 MHRVSDGIGV SWFCRFVGF DDFLHQRMPD ΝVFAGEGMEI QSCHAVQFLT 51 CVFQSRFDVF GQVDLAGVAG ΝDGFRAEADA GQEHFHLFGR GVLRFVEDDV 101 CAVERPAAHI GERGDFΝQAF FΝQLGYTVKA HQVIEGIIKR TEVGVDFLGQ 151 VSGQEAQFLT GFDGRPΝ* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1437>: m517.seq 1 ATGCATCGGG TTTCAGACGG CATTGGAATG TCAGTCGTGT TCTGCCGATT 51 CGTAGGCTTC GACGATTTTT TGCACCAAAG GATGCCGGAC AACGTCTTCG 101 CCGGTAAAGG TGTGGAAATA CAGCCCTTCC ACGTTGTGCA GTTTCTCACG 151 CGCATCTTTT AATCCCGATT TGATGTTTTT GGGCAGGTCG ATTTGGCTGG 201 TGTCGCCGGT AATGACGGCT TTCGCGCCGA AGCCGATGCG GGTCAGGAAC 251 ATTTTCATTT GTTCGGGCGT GGTGTTTTGC GCTTCGTCGA GGATGATGTA 301 TGCGCCGTTG AGCGTCCTGC CGCGCATATA GGCGAGCGGG GCGATTTCAA 351 TCAGGCCTTT TTCAATCAGC TTGGTTACAC GGTCAAAGCC CATCAGGTCA 401 TAGAGGGCAT CATAAAGCGG ACGAAGGTAG GGATCGACTT TCTGGGTCAG 451 GTCTCCGGGC AGGAAGCCCA GTTTCTCGCC GGCTTCGACG GCTGgGCGCA 501 CTAA This corresponds to the amino acid sequence <SEQ ID 1438; ORF 517>: m517.pep 1 MHRVSDGIGM SWFCRFVGF DDFLHQRMPD NVFAGKGVEI QPFHWQFLT 51 RIFXSRFDVF GQVDLAGVAG NDGFRAEADA GQEHFHLFGR GVLRFVEDDV 101 CAVERPAAHI GERGDFNQAF FNQLGYTVKA HQVIEGIIKR TKVGIDFLGQ 151 VSGQEAQFLA GFDGWAH* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 517 shows 92.7% identity over a 164 aa overlap with a predicted ORF (ORF 517.ng) from N gonorrhoeae: m517/g517 10 20 30 40 50 60 m517. pep MHRVSDGIGMSWFCRFVGFDDFLHQRMPDΝVFAGKGVEIQPFHWQFLTRIFXSRFDVF M M M M I M M M M M M M M M M M M M M M M I M I I M M M M M g 517 MHRVSDGI GVS WFCRFVGFDDFLHQRMPDΝVFAGEGME I QS CHAVQFLTCVFQSRFDVF 10 20 30 40 50 60 70 80 90 100 110 120 m517. pep GQVDLAGVAGΝDGFRAEADAGQEHFHLFGRGVLRFVEDDVCAVERPAAHIGERGDFΝQAF MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g517 GQVDLAGVAGΝDGFRAEADAGQEHFHLFGRGVLRFVEDDVCAVERPAAHIGERGDFΝQAF 70 80 90 100 110 120 130 140 150 160 m517.pep FΝQLGYTVKAHQVIEGIIKRTKVGIDFLGQVSGQEAQFLAGFDGWAH I I M i l l I I I I M M M I I M M I M M M M M M I M M M I g517 FΝQLGYTVKAHQVIEGIIKRTEVGVDFLGQVSGQEAQFLTGFDGRPΝ 130 140 150 160 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1439>: a517. seq 1 ATGCATCGGG TTTCAGACGG CATTGGAATG TCAGTCGTGT TCTGCCGATT 51 CGTAGGCTTC GACGATTTTT TGCACCAAAG GATGCCGGAC AACGTCTTCG 101 CCGGTAAAGG TGTGGAAATA CAGCCCTTCC ACGCCGTGCA GTTTCTCACG 151 CGCATCTTTT AATCCCGATT TGATGTTTTT GGGCAGGTCG ATTTGGCTGG 201 TGTCGCCGGT AATGACGGCT TTCGCGCCGA AGCCGATGCG GGTCAGGAAC 251 ATTTTCATTT GTTCGGGCGT GGTGTTTTGC GCTTCGTCGA GGATGATGTA 301 TGCGCCGTTG AGCGTCCTGC CGCGCATATA GGCGAGCGGG GCAATCTCAA 351 TCAGACCTTT TTCAATCAGC TTGGTGACAC GGTCGAAGCC CATCAGGTCA 401 TAGAGGGCAT CATAAAGCGG ACGAAGGTAG GGATCGACTT TCTGGGTCAG 451 GTCACCGGGC AGAAAACCCA GTTTCTCGCC GGCTTCGACG GCAGGCCGCA 501 CTAA This corresponds to the amino acid sequence <SEQ ID 1440; ORF 517.a>: a517.pep 1 MHRVSDGIGM SWFCRFVGF DDFLHQRMPD NVFAGKGVEI QPFHAVQFLT 51 RIF*ΞRFDVF GQVDLAGVAG NDGFRAEADA GQEHFHLFGR GVLRFVEDDV 101 CAVERPAAHI GERGNLNQTF FNQLGDTVEA HQVIEGIIKR TKVGIDFLGQ 151 VTGQKTQFLA GFDGRPH* m517/a517 93.4% identity in 167 aa overlap 10 20 30 40 50 60 m517.pep MHRVSDGIGMSVVFCRFVGFDDFLHQRMPDNVFAGKGVEIQPFHWQFLTRIFXSRFDVF I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I hi I I I I I I I I I I I I I I a517 MHRVSDGIGMSWFCRFVGFDDFLHQRMPDNVFAGKGVEIQPFHAVQFLTRIFXSRFDVF 10 20 30 40 50 60 70 80 90 100 110 120 m517 . pep G Q VDLAGVAGNDGFRAEADAG Q EHFHLFGRGVLRFVEDDVCAVERPAAHIGERGDFNQAF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : : I h I a517 G Q VDLAGVAGNDGFRAEADAG Q EHFHLFGRGVLRFVEDDVCAVERPAAHIGERGNLNQTF 70 80 90 100 110 120 130 140 150 160 m517. pep FNQLGYTVKAHQVIEGIIKRTKVGIDFLGQVSGQEAQFLAGFDGWAHX I I I I I I I : I I I I I I I I I I I I I I I I I I I I I hi h: I I I I I I I I II a517 FNQLGDTVEAHQVIEGIIKRTKVGIDFLGQVTGQKTQFLAGFDGRPHX 130 140 150 160 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1441>: g518. seq 1 atgacgtttt cggcggcaaa gctcaacatt tcggcactga tgttgtgtct 51 ttcggcagga atgaccgttt tactttccgc ttttttactg ctccgaccgg 101 aaggcagcat cttattcaac cattttttca gcataaatat tctgacccga 151 agagcggcat ctccacgggc aaccgtgttc agactgcatc aggcggtacg 201 attccacaag atgccgaaaa ccataagcaa aatgcgtaga aactacgccg 251 tccgaatcac gccgcctcct cgggcggcaa cgcttcatta taacagattg 301 ccccttaaaa aatcagaccc tgcttttgtg gcggagtctg aaatttga This corresponds to the amino acid sequence <SEQ ID 1442; ORF 518.ng>: g518.pep 1 MTFSAAKLΝI SALMLCLSAG MTVLLSAFLL LRPEGSILFΝ HFFSIΝILTR 51 RAASPRATVF RLHQAVRFHK MPKTISKMRR ΝYAVRITPPP RAATLHYΝRL 101 PLKKSDPAFV AESEI* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1443>: m518. seq 1 ATGACGTTTT CGGCGGCAAA GCTCAACATT TCGGCACGGA TGTTGTGTCT 51 TTCGGCAGGA ATGACCGTTT TACTTTCCGC TTTTTTACTG CTCCGACCGG 101 AAGGCAGCAT CTTATTCAAC CATTTTTTCA GCATAAATAT TCTGACCCGA 151 AGAGCGGCAT CTCCACAGGC AACCGTGTTC AGACGGCATC AGGCGCGGTT 201 TGCAAGATGC CGTACCATAA ACAAAAGGCG TAGAAACTAC GCCGTCCGAA 251 TCACGCCGCC CTCGCG.GCG GCAACGCGTC ATTATAACAG ATTGCCCTCC 301 GCGGCAGGCT TAGTGCGGCG GGAGCGCCGC CGTTGCGCAG TAATATTGTC 351 TAACGGGAGG AAAAAATCAG ACCCTGCTTT TGTGGCAGAG TCTGAAATTT 401 GA This corresponds to the amino acid sequence <SEQ ID 1444; ORF 518>: m518.pep 1 MTFSAAKLΝI SARMLCLSAG MTVLLSAFLL LRPEGSILFΝ HFFSIΝILTR 51 RAASPQATVF RRHQARFARC RTIΝKRRRΝY AVRITPPSXA ATRHYΝRLPS 101 AAGLVRRERR RCAVILSΝGR KKSDPAFVAE SEI* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 518 shows 74.1% identity over a 135 aa overlap with a predicted ORF (ORF 518.ng) from N gonorrhoeae: m518/g518 m518.pep MTFSAAKLΝISARMLCLSAGMTVLLSAFLLLRPEGSILFΝHFFSIΝILTRRAASPQATVF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I: I I I I g518 MTFSAAKLΝISALMLCLSAGMTVLLSAFLLLRPEGSILFΝHFFSIΝILTRRAASPRATVF 10 20 30 40 50 60 70 80 90 100 110 m518.pep RRHQA-RFARC-RTIΝKRRRΝYAVRITPPSXAATRHYΝRLPSAAGLVRRERRRCAVILSΝ I III II : : I I : I I I I I I I I I I I I III llllll g518 RLHQAVRFHKMPKTISKMRRΝYAVRITPPPRAATLHYΝRLPL 70 80 90 100 120 130 m518.pep GRKKSDPAFVAESEI g518 —KKSDPAFVAESEI 110 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1445>: a518.seq 1 ATGACGTTTT CGGCGGCAAA GCTCAACATT TCGGCACGGA TGTTGTGTCT 51 TTCGGCAGGA ATGACCGTTT TACTTTCCGC TTTTTTACTG CTCCGACCGG 101 AAGGCAGCAT CTTATTCAAC CATTTTTTCA GCATAAATAT TCTAACCCGA 151 AGAGCGGCAT CTCCACGGGC AACCGTGTTC AGACGGCATC AGGCGGTACG 201 ATTCCGCAAG ATGCCGACCA TAAACAAAAG GCGTAGAAAC TACGCCGTCC 251 GAATCACGCC GTCCTCG.CG GCGGCAACGC GTCATTATAA CAGATTGCCC 301 TCC 351 AAAAAAT CAGACCCTGC TTTTGTGGCA GAGTCTGAAA 401 TTTGA This corresponds to the amino acid sequence <SEQ ID 1446; ORF 518.a>: a518.pep 1 MTFSAAKLΝI SARMLCLΞAG MTVLLSAFLL LRPEGSILFΝ HFFSIΝILTR 51 RAASPRATVF RRHQAVRFRK MPTIΝKRRRΝ YAVRITPSSX AATRHYΝRLP 101 S KKSDPAFVA ESEI* πι518/a518 79.9% identity in 134 aa overlap 10 20 30 40 50 60 m518.pep MTFSAAKLΝISARMLCLSAGMTVLLSAFLLLRPEGSILFΝHFFSIΝILTRRAASPQATVF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I hi I I I a518 MTFSAAKLΝISARMLCLSAGMTVLLSAFLLLRPEGSILFΝHFFSIΝILTRRAASPRATVF 10 20 30 40 50 60 70 80 90 100 110 119 m518. pep RRHQA-RFARCRTIΝKRRRΝYAVRITPPSXAATRHYΝRLPSAAGLVRRERRRCAVILΞΝG I I I I I II : lllllllllllllll lllllllllllll a518 RRHQAVRFRKMPTIΝKRRRΝYAVRITPSΞXAATRHYΝRLPS 70 80 90 100 120 130 m518.pep RKKSDPAFVAESEIX a518 -KKSDPAFVAESEIX 110 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 1447>: g519. seq 1 atggaatttt tcattatctt gttggcagcc gtcgccgttt tcggcttcaa 51 atcctttgtc gtcatccccc agcaggaagt ccacgttgtc gaaaggctcg 101 ggcgtttcca tcgcgccctg acggccggtt tgaatatttt gattcccttt 151 atcgaccgcg tcgcctaccg ccattcgctg aaagaaatcc ctttagacgt 201 acccagccag gtctgcatca cgcgcgataa tacgcaattg actgttgacg 251 gcatcatcta tttccaagta accgatccca aactcgcctc atacggttcg 301 agcaactaca ttatggcaat tacccagctt gcccaaacga cgctgcgttc 351 cgttatcggg cgtatggagt tggacaaaac gtttgaagaa cgcgacgaaa 401 tcaacagtac cgtcgtctcc gccctcgatg aagccgccgg ggcttggggt 451 gtgaaagtcc tccgttacga aatcaaggat ttggttccgc cgcaagaaat 501 ccttcgcgca atgcaggcac aaattaccgc cgaacgcgaa aaacgcgccc 551 gtattgccga atccgaaggc cgtaaaatcg aacaaatcaa ccttgccagt 601 ggtcagcgtg aagccgaaat ccaacaatcc gaaggcgagg ctcaggctgc 651 ggtcaatgcg tccaatgccg agaaaatcgc ccgcatcaac cgcgccaaag 701 gcgaagcgga atccctgcgc cttgttgccg aagccaatgc cgaagccaac 751 cgtcaaattg ccgccgccct tcaaacccaa agcggggcgg atgcggtcaa 801 tctgaagatt gcgggacaat acgttaccgc gttcaaaaat cttgccaaag 851 aagacaatac gcggattaag cccgccaagg ttgccgaaat cgggaaccct 901 aattttcggc ggcatgaaaa attttcgcca gaagcaaaaa cggccaaata 951 a This corresponds to the amino acid sequence <SEQ ID 1448; ORF 519.ng>: g519.pep 1 MEFFIILLAA VAVFGFKSFV VIPQQEVHVV ERLGRFHRAL TAGLΝILIPF 51 IDRVAYRHSL KEIPLDVPSQ VCITRDΝTQL TVDGIIYFQV TDPKLASYGS 101 SNYIMAITQL AQTTLRSVIG RMELDKTFEE RDEINSTVVS ALDEAAGAWG 151 VKVLRYEIKD LVPPQEILRA MQAQITAERE KRARIAESEG RKIEQINLAS 201 GQREAEIQQS EGEAQAAVNA SNAEKIARIN RAKGEAESLR LVAEANAEAN 251 RQIAAALQTQ SGADAVNLKI AGQYVTAFKN LAKEDNTRIK PAKVAEIGNP 301 NFRRHEKFSP EAKTAK* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1449>: m519.seq (partial) 1 ..TCCGTTATCG GGCGTATGGA GTTGGACAAA ACGTTTGAAG AACGCGACGA 51 AATCAACAGT ACTGTTGTTG CGGCTTTGGA CGAGGCGGCC GGGgCTTgGG 101 GTGTGAAGGT TTTGCGTTAT GAGATTAAAG ACTTGGTTCC GCCGCAAGAA 151 ATCCTTCGCT CAATGCAGGC GCAAATTACT GCCGAACGCG AAAAACGCGC 201 CCGTATCGCC GAATCCGAAG GTCGTAAAAT CGAACAAATC AACCTTGCCA 251 GTGGTCAGCG CGAAGCCGAA ATCCAACAAT CCGAAGGCGA GGCTCAGGCT 301 GCGGTCAATG CGTCAAATGC CGAGAAAATC GCCCGCATCA ACCGCGCCAA 351 AGGTGAAGCG GAATCCTTGC GCCTTGTTGC CGAAGCCAAT GCCGAAGCCA 401 TCCGTCAAAT TGCCGCCGCC CTTCAAACCC AAGGCGGTGC GGATGCGGTC 451 AATCTGAAGA TTGCGGAACA ATACGTCGCT GCGTTCAACA ATCTTGCCAA 501 AGAAAGCAAT ACGCTGATTA TGCCCGCCAA TGTTGCCGAC ATCGGCAGCC 551 TGATTTCTGC CGGTATGAAA ATTATCGACA GCAGCAAAAC CGCCAAaTAA This corresponds to the amino acid sequence <SEQ ID 1450; ORF 519>: m519.pep (partial) 1 ..SVIGRMELDK TFEERDEIΝS TWAALDEAA GAWGVKVLRY EIKDLVPPQE 51 ILRSMQAQIT AEREKRARIA ESEGRKIEQI ΝLASGQREAE IQQSEGEAQA 101 AVΝASΝAEKI ARIΝRAKGEA ESLRLVAEAΝ AEAIRQIAAA LQTQGGADAV 151 ΝLKIAEQYVA AFΝΝLAKESΝ TLIMPAΝVAD IGSLISAGMK IIDSSKTAK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 519 shows 87.5% identity over a 200 aa overlap with a predicted ORF (ORF 519.ng) from N gonorrhoeae: m519/g519 10 20 30 m519.pep SVIGRMELDKTFEERDEIΝSTWAALDEAA MMMMMIMMMMMMMIMM g519 YFQVTDPKLASYGSSΝYIMAITQLAQTTLRSVIGRMELDKTFEERDEIΝSTWSALDEAA 90 100 110 120 130 140 40 50 60 70 80 90 m519. pep GAWGVKVLRYEIKDLVPPQEILRSMQAQITAEREKRARIAESEGRKIEQIΝLASGQREAE MMMMMMMMMMMIMMMMMMMMMMMMMMIMMMM g519 GAWGVKVLRYEIKDLVPPQEILRAMQAQITAEREKRARIAESEGRKIEQIΝLASGQREAE 150 160 170 180 190 200 100 110 120 130 140 150 m519. pep IQQSEGEAQAAVΝASΝAEKIARIΝRAKGEAESLRLVAEAΝAEAIRQIAAALQTQGGADAV MMMMMMMMMMMMMMMMMIMMMM MIIMMMMMM g519 IQQSEGEAQAAVΝASΝAEKIARIΝRAKGEAESLRLVAEAΝAEAΝRQIAAALQTQSGADAV 210 220 230 240 250 260 160 170 180 190 200 m519.pep ΝLKIAEQYVAAFΝΝLAKESΝTLIMPAΝVADIGSL-ISAGMKIIDSSKTAK M i l l I M M I M M M M I I I I M I M h : h M i l l g519 ΝLKIAGQYVTAFKΝLAKEDΝTRIKPAKVAEIGΝPΝFRRHEKFSPEAKTAK 270 280 290 300 310 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1451>: a519.seq 1 ATGGAATTTT TCATTATCTT GCTGGCAGCC GTCGTTGTTT TCGGCTTCAA 51 ATCCTTTGTT GTCATCCCAC AGCAGGAAGT CCACGTTGTC GAAAGGCTCG 101 GGCGTTTCCA TCGCGCCCTG ACGGCCGGTT TGAATATTTT GATTCCCTTT 151 ATCGACCGCG TCGCCTACCG CCATTCGCTG AAAGAAATCC CTTTAGACGT 201 ACCCAGCCAG GTCTGCATCA CGCGCGACAA TACGCAGCTG ACTGTTGACG 251 GTATCATCTA TTTCCAAGTA ACCGACCCCA AACTCGCCTC ATACGGTTCG 301 AGCAACTACA TTATGGCGAT TACCCAGCTT GCCCAAACGA CGCTGCGTTC 351 CGTTATCGGG CGTATGGAAT TGGACAAAAC GTTTGAAGAA CGCGACGAAA 401 TCAACAGCAC CGTCGTCTCC GCCCTCGATG AAGCCGCCGG AGCTTGGGGT 451 GTGAAGGTTT TGCGTTATGA GATTAAAGAC TTGGTTCCGC CGCAAGAAAT 501 CCTTCGCTCA ATGCAGGCGC AAATTACTGC TGAACGCGAA AAACGCGCCC 551 GTATCGCCGA ATCCGAAGGT CGTAAAATCG AACAAATCAA CCTTGCCAGT 601 GGTCAGCGCG AAGCCGAAAT CCAACAATCC GAAGGCGAGG CTCAGGCTGC 651 GGTCAATGCG TCAAATGCCG AGAAAATCGC CCGCATCAAC CGCGCCAAAG 701 GTGAAGCGGA ATCCTTGCGC CTTGTTGCCG AAGCCAATGC CGAAGCCATC 751 CGTCAAATTG CCGCCGCCCT TCAAACCCAA GGCGGTGCGG ATGCGGTCAA 801 TCTGAAGATT GCGGAACAAT ACGTCGCCGC GTTCAACAAT CTTGCCAAAG 851 AAAGCAATAC GCTGATTATG CCCGCCAATG TTGCCGACAT CGGCAGCCTG 901 ATTTCTGCCG GTATGAAAAT TATCGACAGC AGCAAAACCG CCAAATAA This corresponds to the amino acid sequence <SEQ ID 1452; ORF 519.a>: a519.pep 1 MEFFIILLAA VVVFGFKSFV VIPQQEVHVV ERLGRFHRAL TAGLNILIPF 51 IDRVAYRHSL KEIPLDVPSQ VCITRDNTQL TVDGIIYFQV TDPKLASYGS 101 SNYIMAITQL AQTTLRSVIG RMELDKTFEE RDEINSTVVS ALDEAAGAWG 151 VKVLRYEIKD LVPPQEILRS MQAQITAERE KRARIAESEG RKIEQINLAS 201 GQREAEIQQS EGEAQAAVNA SNAEKIARIN RAKGEAESLR LVAEANAEAI 251 RQIAAALQTQ GGADAVNLKI AEQYVAAFNN LAKESNTLIM PANVADIGSL 301 ISAGMKIIDS SKTAK* 519/a519 99.5% identity in 199 aa overlap 10 20 30 m519.pep SVIGRMELDKTFEERDEINSTVVAALDEAA I I I I I I I I I I I I I I I I I I I I I I h I I I I I I a519 YFQVTDPKLASYGSΞNYIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVSALDEAA 90 100 110 120 130 140 40 50 60 70 80 90 m519.pep GAWGVKVLRYEIKDLVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a519 GAWGVKVLRYEIKDLVPPQEILRSMQAQITAEREKRARIAEΞEGRKIEQINLAΞGQREAE 150 160 170 180 190 200 100 110 120 130 140 150 m519.pep IQQΞEGEAQAAVNASNAEKIARINRAKGEAEΞLRLVAEANAEAIRQIAAALQTQGGADAV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a519 IQQSEGEAQAAVNAΞNAEKIARINRAKGEAEΞLRLVAEANAEAIRQIAAALQTQGGADAV 210 220 230 240 250 260 160 170 180 190 200 m519.pep NLKIAEQYVAAFNNLAKEΞNTLIMPANVADIGSLISAGMKIIDSΞKTAKX I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a519 NLKIAEQYVAAFNNLAKESNTLIMPANVADIGΞLISAGMKIIDΞSKTAKX 270 280 290 300 310 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1453>: g519-l.seq 1 ATGGAATTTT TCATTATCTT GTTGGCAGCC GTCGCCGTTT TCGGCTTCAA 51 ATCCTTTGTC GTCATCCCCC AGCAGGAAGT CCACGTTGTC GAAAGGCTCG 101 GGCGTTTCCA TCGCGCCCTG ACGGCCGGTT TGAATATTTT GATTCCCTTT 151 ATCGACCGCG TCGCCTACCG CCATTCGCTG AAAGAAATCC CTTTAGACGT 201 ACCCAGCCAG GTCTGCATCA CGCGCGATAA TACGCAATTG ACTGTTGACG 251 GCATCATCTA TTTCCAAGTA ACCGATCCCA AACTCGCCTC ATACGGTTCG 301 AGCAACTACA TTATGGCAAT TACCCAGCTT GCCCAAACGA CGCTGCGTTC 351 CGTTATCGGG CGTATGGAGT TGGACAAAAC GTTTGAAGAA CGCGACGAAA 401 TCAACAGTAC CGTCGTCTCC GCCCTCGATG AAGCCGCCGG GGCTTGGGGT 451 GTGAAAGTCC TCCGTTACGA AATCAAGGAT TTGGTTCCGC CGCAAGAAAT 501 CCTTCGCGCA ATGCAGGCAC AAATTACCGC CGAACGCGAA AAACGCGCCC 551 GTATTGCCGA ATCCGAAGGC CGTAAAATCG AACAAATCAA CCTTGCCAGT 601 GGTCAGCGTG AAGCCGAAAT CCAACAATCC GAAGGCGAGG CTCAGGCTGC 651 GGTCAATGCG TCCAATGCCG AGAAAATCGC CCGCATCAAC CGCGCCAAAG 701 GCGAAGCGGA ATCCCTGCGC CTTGTTGCCG AAGCCAATGC CGAAGCCATC 751 CGTCAAATTG CCGCCGCCCT TCAAACCCAA GGCGGGGCGG ATGCGGTCAA 801 TCTGAAGATT GCGGAACAAT ACGTAGCCGC GTTCAACAAT CTTGCCAAAG 851 AAAGCAATAC GCTGATTATG CCCGCCAATG TTGCCGACAT CGGCAGCCTG 901 ATTTCTGCCG GCATGAAAAT TATCGACAGC AGCAAAACCG CCAAATAA This corresponds to the amino acid sequence <SEQ ID 1454; ORF 519-l.ng>: g519-l.pep 1 MEFFIILLAA VAVFGFKSFV VIPQQEVHVV ERLGRFHRAL TAGLNILIPF 51 IDRVAYRHSL KEIPLDVPSQ VCITRDNTQL TVDGIIYFQV TDPKLASYGS 101 SNYIMAITQL AQTTLRSVIG RMELDKTFEE RDEINSTVVS ALDEAAGAWG 151 VKVLRYEIKD LVPPQEILRA MQAQITAERE KRARIAESEG RKIEQINLAS 201 GQREAEIQQS EGEAQAAVNA SNAEKIARIN RAKGEAESLR LVAEANAEAI 251 RQIAAALQTQ GGADAVNLKI AEQYVAAFNN LAKESNTLIM PANVADIGSL 301 ISAGMKIIDS SKTAK* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1455>: m519-l.seq 1 ATGGAATTTT TCATTATCTT GTTGGTAGCC GTCGCCGTTT TCGGTTTCAA 51 ATCCTTTGTT GTCATCCCAC AACAGGAAGT CCACGTTGTC GAAAGGCTGG 101 GGCGTTTCCA TCGCGCCCTG ACGGcCGGTT TGAATATTTT GATTCCCTTT 151 ATCGACCGCG TCGCCTACCG CCATTCGCTG AAAGAAATCC CTTTAGACGT 201 ACCCAGCCAG GTCTGCATCA CGCGCGACAA TACGCAGCTG ACTGTTGACG 251 GCATCATCTA TTTCCAAGTA ACCGACCCCA AACTCGCCTC ATACGGTTCG 301 AGCAACTACA TTATGGCGAT TACCCAGCTT GCCCAAACGA CGCTGCGTTC 351 CGTTATCGGG CGTATGGAGT TGGACAAAAC GTTTGAAGAA CGCGACGAAA 401 TCAACAGTAC TGTTGTTGCG GCTTTGGACG AGGCGGCCGG GGCTTGGGGT 451 GTGAAGGTTT TGCGTTATGA GATTAAAGAC TTGGTTCCGC CGCAAGAAAT 501 CCTTCGCTCA ATGCAGGCGC AAATTACTGC CGAACGCGAA AAACGCGCCC 551 GTATCGCCGA ATCCGAAGGT CGTAAAATCG AACAAATCAA CCTTGCCAGT 601 GGTCAGCGCG AAGCCGAAAT CCAACAATCC GAAGGCGAGG CTCAGGCTGC 651 GGTCAATGCG TCAAATGCCG AGAAAATCGC CCGCATCAAC CGCGCCAAAG 701 GTGAAGCGGA ATCCTTGCGC CTTGTTGCCG AAGCCAATGC CGAAGCCATC 751 CGTCAAATTG CCGCCGCCCT TCAAACCCAA GGCGGTGCGG ATGCGGTCAA 801 TCTGAAGATT GCGGAACAAT ACGTCGCTGC GTTCAACAAT CTTGCCAAAG 851 AAAGCAATAC GCTGATTATG CCCGCCAATG TTGCCGACAT CGGCAGCCTG 901 ATTTCTGCCG GTATGAAAAT TATCGACAGC AGCAAAACCG CCAAATAA This corresponds to the amino acid sequence <SEQ ID 1456; ORF 519-1>: m519-l. 1 MEFFIILLVA VAVFGFKSFV VIPQQEVHVV ERLGRFHRAL TAGLΝILIPF 51 IDRVAYRHSL KEIPLDVPSQ VCITRDΝTQL TVDGIIYFQV TDPKLASYGS 101 SΝYIMAITQL AQTTLRSVIG RMELDKTFEE RBEIΝΞTWA ALDEAAGAWG 151 VKVLRYEIKD LVPPQEILRS MQAQITAERE KRARIAESEG RKIEQIΝLAS 201 GQREAEIQQS EGEAQAAVΝA SΝAEKIARIΝ RAKGEAESLR LVAEANAEAI 251 RQIAAALQTQ GGADAVNLKI AEQYVAAFNN LAKESNTLIM PANVADIGSL 301 ISAGMKIIDS SKTAK* m519-l/g519-l 99.0% identity in 315 aa overlap 10 20 30 40 50 60 g519-l .pep MEFFIILLAAVAVFGFKSFWIPQQEVHWERLGRFHRALTAGLNILIPFIDRVAYRHSL m519-l MEFFIILLVAVAVFGFKSFWIPQQEVHWERLGRFHRALTAGLNILIPFIDRVAYRHSL 10 20 30 40 50 60 70 80 90 100 110 120 g519-1.pep KEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIG m519 -l KEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLAΞYGSSNYIMAITQLAQTTLRSVIG 70 80 90 100 110 120 130 140 150 160 170 180 g519 -l . pep RMELDKTFEERDEINSTWSALDEAAGAWGVKVLRYEIKDLVPPQEILRAMQAQITAERE m519 - l RMELDKTFEERDEINSTWAALDEAAGAWGVKVLRYEIKDLVPPQEILRSMQAQITAERE 130 140 150 160 170 180 190 200 210 220 230 240 g519 -l . pep KRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR 1 1 1 E 1 F 1 1 1 j E 1 1 1 1 1 1 1 1 J F 1 1 f F I ! 1 1 1 1 1 F F I F F I j 1 1 1 1 1 1 i 1 1 1 1 [ f I [ 1 1 1 1 i I m519-l KRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR 190 200 210 220 230 240 250 260 270 280 290 300 g519-l .pep LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL m519-1 LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL 250 260 270 280 290 300 310 g519-l.pep ISAGMKIIDSSKTAKX m519 -l ISAGMKIIDSSKTAKX 310 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1457>: a519-l.seq 1 ATGGAATTTT TCATTATCTT GCTGGCAGCC GTCGTTGTTT TCGGCTTCAA 51 ATCCTTTGTT GTCATCCCAC AGCAGGAAGT CCACGTTGTC GAAAGGCTCG 101 GGCGTTTCCA TCGCGCCCTG ACGGCCGGTT TGAATATTTT GATTCCCTTT 151 ATCGACCGCG TCGCCTACCG CCATTCGCTG AAAGAAATCC CTTTAGACGT 201 ACCCAGCCAG GTCTGCATCA CGCGCGACAA TACGCAGCTG ACTGTTGACG 251 GTATCATCTA TTTCCAAGTA ACCGACCCCA AACTCGCCTC ATACGGTTCG 301 AGCAACTACA TTATGGCGAT TACCCAGCTT GCCCAAACGA CGCTGCGTTC 351 CGTTATCGGG CGTATGGAAT TGGACAAAAC GTTTGAAGAA CGCGACGAAA 401 TCAACAGCAC CGTCGTCTCC GCCCTCGATG AAGCCGCCGG AGCTTGGGGT 451 GTGAAGGTTT TGCGTTATGA GATTAAAGAC TTGGTTCCGC CGCAAGAAAT 501 CCTTCGCTCA ATGCAGGCGC AAATTACTGC TGAACGCGAA AAACGCGCCC 551 GTATCGCCGA ATCCGAAGGT CGTAAAATCG AACAAATCAA CCTTGCCAGT 601 GGTCAGCGCG AAGCCGAAAT CCAACAATCC GAAGGCGAGG CTCAGGCTGC 651 GGTCAATGCG TCAAATGCCG AGAAAATCGC CCGCATCAAC CGCGCCAAAG 701 GTGAAGCGGA ATCCTTGCGC CTTGTTGCCG AAGCCAATGC CGAAGCCATC 751 CGTCAAATTG CCGCCGCCCT TCAAACCCAA GGCGGTGCGG ATGCGGTCAA 801 TCTGAAGATT GCGGAACAAT ACGTCGCCGC GTTCAACAAT CTTGCCAAAG 851 AAAGCAATAC GCTGATTATG CCCGCCAATG TTGCCGACAT CGGCAGCCTG 901 ATTTCTGCCG GTATGAAAAT TATCGACAGC AGCAAAACCG CCAAATAA This corresponds to the amino acid sequence <SEQ ID 1458; ORF 519-1. a>: a519-l.pep. 1 MEFFIILLAA VVVFGFKSFV VIPQQEVHVV ERLGRFHRAL TAGLΝILIPF 51 IDRVAYRHSL KEIPLDVPSQ VCITRDΝTQL TVDGIIYFQV TDPKLASYGS 101 SΝYIMAITQL AQTTLRSVIG RMELDKTFEE RDEINSTVVS ALDEAAGAWG 151 VKVLRYEIKD LVPPQEILRS MQAQITAERE KRARIAESEG RKIEQINLAS 201 GQREAEIQQS EGEAQAAVNA SNAEKIARIN RAKGEAESLR LVAEANAEAI 251 RQIAAALQTQ GGADAVNLKI AEQYVAAFNN LAKESNTLIM PANVADIGSL 301 ISAGMKIIDS SKTAK* m519-l/a519-l 99.0% identity in 315 aa overlap 10 20 30 40 50 60 a519-l .pep MEFFIILLAAVWFGFKSFWIPQQEVHWERLGRFHRALTAGLNILIPFIDRVAYRHSL m519 - 1 MEFFIILLVAVAVFGFKSFWI PQQEVHWERLGRFHRALTAGLNILI PFIDRVAYRHSL 10 20 30 40 50 60 70 80 90 100 110 120 a519 - l . pep KEIPLDVPΞQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIG m519 - l KEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSNYIMAITQLAQTTLRSVIG 70 80 90 100 110 120 130 140 150 160 170 180 a519 -l . pep RMELDKTFEERDEINSTWSALDEAAGAWGVKVLRYEIKDLVPPQEILRSMQAQITAERE m519 -l RMELDKTFEERDEINΞTWAALDEAAGAWGVKVLRYEIKDLVPPQEILRSMQAQITAERE 130 140 150 160 170 180 190 200 210 220 230 240 a519 - l . pep KRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAVNASNAEKIARINRAKGEAEΞLR m519-l KRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAVNASNAEKIARINRAKGEAESLR 190 200 210 220 230 240 250 260 270 280 290 300 a519-l .pep LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKEΞNTLIMPANVADIGSL m519-l LVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVAAFNNLAKESNTLIMPANVADIGSL 250 260 270 280 290 300 310 a519-l.pep ISAGMKIIDSSKTAKX m519-l ISAGMKIIDSSKTAKX 310 Expression of ORF 519 The primer described in Table 1 for ORF 519 was used to locate and clone ORF 519. ORF 519 was cloned in pET and pGex vectors and expressed in E.coli as above described. The products of protein expression and purification were analyzed by SDS-PAGE. Figure 4A shows the results of affinity purification, and Figure 4B shows the expression in E.coli. Purified Nis-fusion protein was used to immunize mice whose sera were used for ELISA (positive result), FACS analysis (Figure 4C), western blot (Figure IE), and a bactericidal assay (Figure 4D). These experiments confirm that 519 is a surface-exposed protein and that it is a useful immunogen. The hydrophilicity plots, antigenic index, and amphipatic regions of ORF 519 are provided in Figure 8. The AMPHI program is used to predict putative T-cell epitopes (Gao et al 1989, J. Immunol 143:3007; Roberts et al. 1996, AIDS Res Human Retroviruses 12:593; Quakyi et al. 1992, Scand J Immunol Suppl 11 :9). The nucleic acid sequence of ORF 519 and the amino acid sequence encoded thereby as provided herein. The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1459>: g520.seq 1 atgcctgcgc ttctttcaat acgtcgggca aacgcgctgc ctttttcgcg 51 catttcggaa aggatgaagt tgctggtgcc gttaataatg ccggcgatgg 101 atttaatcct gtttgccgcc aaaccttcgc gcacggcttt gatgattggg 151 ataccgcccg ctactgccgc ttcaaattgg acgatgacgt tttgtttttc 201 cgccagcggg aagatttcgt tgccgtattc ggcgagcagt tttttgttgg 251 cggtaacgat gtgtttgccg ttttcaatgg ctttcaacac cgcttctttg 301 gcaatgcccg tgccgccgaa caattcgacc aagacatcga cgtctttacg 351 cgcgaacagt tcgaacggat cttttgacaa gggcgggcga cgggccgatt 401 ttggcgggct ttttcttcgc ttaagtcgca catggcagaa atacggattt 451 cgcgccccaa gcggcgggaa atttcctctg cgttgtcccg caacacggca 501 gccgcaccgc cgccgaccgt acctaagcct aaaagaccga tgtttactgg 551 cttcattgtg tctccttgta agccgactga aatgtaaata ttga This corresponds to the amino acid sequence <SEQ ID 1460; ORF 520.ng>: g520.pep 1 MPALLSIRRA ΝALPFSRISE RMKLLVPLIM PAMDLILFAA KPSRTALMIG 51 IPPATAASΝW TMTFCFSASG KISLPYSASS FLLAVTMCLP FSMAFΝTASL 101 AMPVPPΝΝST KTSTSLRAΝS SΝGSFDKGGR RADFGGLFLR LSRTWQKYGF 151 RAPSGGKFPL RCPATRQPHR RRPYLSLKDR CLLASLCLLV SRLKCKY* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1461>: m520. seq 1 ATGCCTGCGC TTCTTTCAGT ACATCG.GCA AACGCGCTGC CTTTTTCGCG 51 CATTTCGGrk AGGATGAAGT TGCTGGTGCC GTTAATAATG CCGGCGATGG 101 ATTTAATCCT GTTTGCCGCC AAACCTTCGC GCAGGGCTTT GATGATTGGG 151 ATACCGCCCG CTACTGCCGC TTCAAATTGG ACGATGACGT TTTGTTTTTC 201 CGCCAGCGGG AAGATTTCGT TGCCGTATTC GGCGAGCAGT TTTTTGTTGG 251 CGGTAACGAT GTGTTTGCCG TTTTCAATGG CTTTCAACAC CGCATCTTTG 301 GCAATGCCGG TACCGCCGaA CAATTCGACG ACGACATCGA CGTCTTCACG 351 TGCGACCAGT TCGAACGGAT CTTTGACAAA GGCTGc . CGG ACGGGCAGGT 401 TTGTCGGGCT TTTTCTTCAC TCAAATCGCA CACGGCAGAA ATACGGATTT 451 CGCGCCCCAA GCGACGGGAA ATTTCCTCCG CGTTGTCsCG CAACACGGCA 501 GCCGTACCGC CGCCGACCGT ACCCAAACCT AAAAGACCGA TGTTTACTGG 551 CTTCATTGTG TCTCCTTGTA AGCCGACTGA AATGTAAATA TTGA This corresponds to the amino acid sequence <SEQ ID 1462; ORF 520>: m520.pep 1 MPALLSVHXA NALPFSRISX RMKLLVPLIM PAMDLILFAA KPSRRALMIG 51 IPPATAASNW TMTFCFSASG KISLPYSASS FLLAVTMCLP FSMAFNTASL 101 AMPVPPNNST TTSTSSRATS SNGSLTKAXR TGRFVGLFLH SNRTRQKYGF 151 RAPSDGKFPP RCXATRQPYR RRPYPNLKDR CLLASLCLLV SRLKCKY* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 520 shows 87.3% identity over a 197 aa overlap with a predicted ORF (ORF 520.ng) from N. gonorrhoeae: m520/g520 10 20 30 40 50 60 m520.pep MPALLSVHRAΝALPFSRISXRMKLLVPLIMPAMDLILFAAKPSRRALMIGIPPATAASΝW M M I h M M M M M M M M M M M M M M M M M M I M M M M M M M g520 MPALLSIRRAΝALPFSRISERMKLLVPLIMPAMDLILFAAKPSRTALMIGIPPATAASΝW 10 20 30 40 50 60 70 80 90 100 110 120 m520.pep TMTFCFSASGKISLPYSASSFLLAVTMCLPFSMAFΝTASLAMPVPPΝΝSTTTSTSSRATS MMMIMMMMMMMMMMMMMMMMMMMMMI MM MM g520 TMTFCFSASGKISLPYSASSFLLAVTMCLPFSMAFΝTASLAMPVPPΝΝSTKTSTSLRAΝS 70 80 90 100 110 120 130 140 150 160 170 180 m520.pep SΝGSLTKAARTGRFVGLFLHSΝRTRQKYGFRAPSDGKFPPRCXATRQPYRRRPYPΝLKDR M M : M M : I M i : M l 1 1 ! 1 1 1 1 i I l l l l I I M I M M I I M M i l l g520 SΝGSFDKGGRRADFGGLFLRLSRTWQKYGFRAPSGGKFPLRCPATRQPHRRRPYLSLKDR 130 140 150 160 170 180 190 m520. pep CLLASLCLLVSRLKCKY MMMMMMIMM g520 CLLASLCLLVSRLKCKY 190 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1463>: a520. seq 1 ATGCCTGCGC TTCTTTCAGT ACATCGG.CA AACGCGCTGC CTTTTTCGCG 51 CATTTCGGAG AGGATGAAGT TGCTGGTGCC GTTAATAATG CCGGCGATGG 101 ATTTAATCCT GTTTGCCGCC AAACCTTCGC GCAGGGCTTT GATGATTGGG 151 ATACCGCCCG CTACTGCCGC TTCAAATTGG ACGATGACGT TTTGTTTTTC 201 CGCCAGCGGG AAGATTTCGT TGCCGTATTC GGCGAGCAGT TTTTTGTTGG 251 CGGTAACGAT GTGTTTGCCG TTTTCAATGG CTTTCAACAC CGCATCTTTG 301 GCAATGCCGG TACCGCCGAA CAATTCGACG ACGACATCGA CGTCTTCACG 351 TGCGACCAGT TCGAACGGAT CTTTGACAAA GGCTG .. CGG ACGGGCAGGT 401 TTGTCGGGCT TTTTCTTCAC TCAAATCGCA CACGGCAGAA ATACGGATTT 451 CGCGCCCCAA GCGACGGGAA ATTTCCTCCG CGTTGTCCCG CAACACGGCA 501 GCCGTACCGC CGCCGACCGT ACCCAAACCT AAAAGACCGA TGTTTACTGG 551 CTTCATTGTG TCTCCTTGTA AGCCGACTGA AATGTAAATA TTGA This corresponds to the amino acid sequence <SEQ ID 1464; ORF 520. a>: a520.pep 1 MPALLSVHRX ΝALPFSRISE RMKLLVPLIM PAMDLILFAA KPSRRALMIG 51 IPPATAASNW TMTFCFSASG KISLPYSASS FLLAVTMCLP FSMAFNTASL 101 AMPVPPNNST TTSTSSRATS SNGSLTKAXR TGRFVGLFLH SNRTRQKYGF 151 RAPSDGKFPP RCPATRQPYR RRPYPNLKDR CLLASLCLLV SRLKCKY* m520/a520 98.0% identity in 197 aa overlap 10 20 30 40 50 60 m520.pep MPALLSVHXANALPFSRISXRMKLLVPLIMPAMDLILFAAKPSRRALMIGIPPATAASNW llllllll lllllllll I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a520 MPALLSVHRXNALPFSRISERMKLLVPLIMPAMDLILFAAKPSRRALMIGIPPATAASNW 10 20 30 40 50 60 70 80 90 100 110 120 m520.pep TMTFCFSASGKISLPYSASSFLLAVTMCLPFSMAFNTASLAMPVPPNNSTTTSTSSRATS I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a520 TMTFCFSAΞGKISLPYSASSFLLAVTMCLPFSMAFNTASLAMPVPPNNSTTTSTSSRATS 70 80 90 100 110 120 130 140 150 160 170 180 m520.pep SNGSLTKAXRTGRFVGLFLHSNRTRQKYGFRAPSDGKFPPRCXATRQPYRRRPYPNLKDR I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I lllllllllllllllll a520 SNGSLTKAXRTGRFVGLFLHSNRTRQKYGFRAPSDGKFPPRCPATRQPYRRRPYPNLKDR 130 140 150 160 170 180 190 m520.pep CLLASLCLLVSRLKCKYX I I I I I I I I I I I I I I I I I I a520 CLLASLCLLVSRLKCKYX 190 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1465>: g520-l.seq 1 ATGAAGTTGC TGGTGCCGTT AATAATGCCG GCGATGGATT TAATCCTGTT 51 TGCCGCCAAA CCTTCGCGCA GGGCTTTGAT GATTGGGATA CCGCCCGCTA 101 CTGCCGCTTC AAATTGGACG ATGACGTTTT GTTTTTCCGC CAGCGGGAAG 151 ATTTCGTTGC CGTATTCGGC GAGCAGTTTT TTGTTGGCGG TAACGATGTG 201 TTTGCCGTTT TCAATGGCTT TCAACACCGC TTCTTTGGCA ATGCCCGTGC 251 CGccgAACAA TTCGACGACG ACATCGACGT CTTTACGCGC GACCAGTtCG 301 AACGGATCTT TGACAAAGGC GGCGGACGGG CAGATTTGGC GGGCTTTTTC 351 TTCGCTTAAG TCGCACATGG CAGAAATACG GATTTCGCGC CCCAAGCGGC 401 GGGAAATTTC CTCTGCGTTG TCCCGCAACA CGGCAGCCGC ACCGCCGCCG 451 ACCgTACCTA AGCCTAAAAG ACCGATGTTT ACTGGCTTCA TTGTGTCTCC 501 TTGTAAGCCG ACTGAAATGT AA This corresponds to the amino acid sequence <SEQ ID 1466; ORF 520-1. ng>: g520-l.pep 1 MKLLVPLIMP AMDLILFAAK PSRRALMIGI PPATAAΞΝWT MTFCFSAΞGK 51 ISLPYSAΞSF LLAVTMCLPF SMAFΝTASLA MPVPPΝΝSTT TSTSLRATSS 101 ΝGSLTKAADG QIWRAFSΞLK SHMAEIRISR PKRREIΞSAL SRΝTAAAPPP 151 TVPKPKRPMF TGFIVΞPCKP TEM* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1467>: m520-l .seq 1 ATGAAGTTGC TGGTGCCGTT AATAATGCCG GCGATGGATT TAATCCTGTT 51 TGCCGCCAAA CCTTCGCGCA GGGCTTTGAT GATTGGGATA CCGCCCGCTA 101 CTGCCGCTTC AAATTGGACG ATGACGTTTT GTTTTTCCGC CAGCGGGAAG 151 ATTTCGTTGC CGTATTCGGC GAGCAGTTTT TTGTTGGCGG TAACGATGTG 201 TTTGCCGTTT TCAATGGCTT TCAACACCGC ATCTTTGGCA ATGCCGGTAC 251 CGCCGAACAA TTCGACGACG ACATCGACGT CTTCACGTGC GACCAGTTCG 301 AACGGATCTT TGACAAAGGC TGCGGACGGG CAGGTTTGTC GGGCTTTTTC 351 TTCACTCAAA TCGCACACGG CAGAAATACG GATTTCGCGC CCCAAGCGAC 401 GGGAAATTTC CTCCGCGTTG TCCCGCAACA CGGCAGCCGT ACCGCCGCCG 451 ACCGTACCCA AACCTAAAAG ACCGATGTTT ACTGGCTTCA TTGTGTCTCC 501 TTGTAAGCCG ACTGAAATGT AA This corresponds to the amino acid sequence <SEQ ID 1468; ORF 520-l>: m520-l .pep 1 MKLLVPLIMP AMDLILFAAK PSRRALMIGI PPATAASΝWT MTFCFΞASGK 51 ISLPYSASSF LLAVTMCLPF SMAFΝTAΞLA MPVPPΝΝSTT TSTSSRATSS 101 NGΞLTKAADG QVCRAFSSLK SHTAEIRISR PKRREISSAL SRNTAAVPPP 151 TVPKPKRPMF TGFIVSPCKP TEM* g520-l/m520-l 97.1% identity in 173 aa overlap 10 20 30 40 50 60 g520-l.pep MKLLVPLIMPAMDLILFAAKPSRRALMIGIPPATAASNWTMTFCFSASGKISLPYSASSF I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m520-l MKLLVPLIMPAMDLILFAAKPSRRALMIGIPPATAASNWTMTFCFSASGKISLPYSASSF 10 20 30 40 50 60 70 80 90 100 110 120 g520-l .pep LLAVTMCLPFSMAFNTASLAMPVPPNNSTTTSTSLRATSSNGSLTKAADGQIWRAFSSLK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I: l l l l l l l m520-l LLAVTMCLPFSMAFNTAΞLAMPVPPNNSTTTSTSSRATSSNGSLTKAADGQVCRAFSSLK 70 80 90 100 110 120 130 140 150 160 170 g520-l .pep SHMAEIRISRPKRREISSALSRNTAAAPPPTVPKPKRPMFTGFIVSPCKPTEMX II I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I m520-l SHTAEIRISRPKRREISSALSRNTAAVPPPTVPKPKRPMFTGFIVSPCKPTEMX 130 140 150 160 170 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1469>: a520-l.seq 1 ATGAAGTTGC TGGTGCCGTT AATAATGCCG GCGATGGATT TAATCCTGTT 51 TGCCGCCAAA CCTTCGCGCA GGGCTTTGAT GATTGGGATA CCGCCCGCTA 101 CTGCCGCTTC AAATTGGACG ATGACGTTTT GTTTTTCCGC CAGCGGGAAG 151 ATTTCGTTGC CGTATTCGGC GAGCAGTTTT TTGTTGGCGG TAACGATGTG 201 TTTGCCGTTT TCAATGGCTT TCAACACCGC ATCTTTGGCA ATGCCGGTAC 251 CGCCGAACAA TTCGACGACG ACATCGACGT CTTCACGTGC GACCAGTTCG 301 AACGGATCTT TGACAAAGGC TGCGGACGGG CAGGTTTGTC GGGCTTTTTC 351 TTCACTCAAA TCGCACACGG CAGAAATACG GATTTCGCGC CCCAAGCGAC 401 GGGAAATTTC CTCCGCGTTG TCCCGCAACA CGGCAGCCGT ACCGCCGCCG 451 ACCGTACCCA AACCTAAAAG ACCGATGTTT ACTGGCTTCA TTGTGTCTCC 501 TTGTAAGCCG ACTGAAATGT AA This corresponds to the amino acid sequence <SEQ ID 1470; ORF 520-l.a>: a520-l.pep 1 MKLLVPLIMP AMDLILFAAK PSRRALMIGI PPATAAΞΝWT MTFCFSASGK 51 ISLPYΞASSF LLAVTMCLPF SMAFΝTASLA MPVPPΝΝSTT TSTSSRATSS 101 ΝGSLTKAADG QVCRAFSSLK SHTAEIRISR PKRREISSAL SRNTAAVPPP 151 TVPKPKRPMF TGFIVSPCKP TEM* m520-l/a520-l 100.0% identity in 173 aa overlap 10 20 30 40 50 60 a520-l.pep MKLLVPLIMPAMDLILFAAKPΞRRALMIGIPPATAASNWTMTFCFSASGKISLPYΞASSF I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m520-l MKLLVPLIMPAMDLILFAAKPSRRALMIGIPPATAASNWTMTFCFSASGKISLPYSASSF 10 20 30 40 50 60 70 80 90 100 110 120 a520-l.pep LLAVTMCLPFSMAFNTASLAMPVPPNNSTTTΞTSSRATSΞNGΞLTKAADGQVCRAFSSLK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m520-l LLAVTMCLPFSMAFNTAΞLAMPVPPNNSTTTSTΞΞRATSΞNGSLTKAADGQVCRAFSSLK 70 80 90 100 110 120 130 140 150 160 170 a520-l.pep SHTAEIRISRPKRREIΞSALSRNTAAVPPPTVPKPKRPMFTGFIVΞPCKPTEMX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m520-l SHTAEIRISRPKRREISSALSRNTAAVPPPTVPKPKRPMFTGFIVSPCKPTEMX 130 140 150 160 170 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1471>: g521.seq 1 ATGAAATCAA AACTCCCCTT AATCCTAATC AACCTTTCCC TGATTTCAAG 51 CCCATTGGGT GCGAATGCGG CCAAAATCTA TACCTGCACA ATCAACGGAG 101 AAACCGTTTA CACCACCAAG CCGTCTAAAA GCTGCCACTC AACCGATTTG 151 CCCCCAATCG GCAACTACAG CAGCGAACGC TATATCCTGC CCCAAACTCC 201 CGAACCGGCA CCATCACCGT CAAACGGCGG ACAGGCTGTC AAATATAAAG 251 CCCCGGTCAA AACAGTATCC AAGCCGGCAA AATCCAATAC GCCGCCTCAA 301 CAAGCACCTG TAAATAACAG CAGACGCTCC ATTCTcgaag caGaattaag 351 cAatgaacgc aaagccctGa ctGaAGCCCA AAAAATGTTA TCACAagcac 401 gtCtGGCAAA AGGCGgcaAC AtcaaCCatc aaaAaatcaa cgcattgtaa 451 AGCAATGTTt tggacAGACA GCAAAATaTC Caagcactgc aaaGAgAATt 501 GGGACGTATG TAA This corresponds to the amino acid sequence <SEQ ID 1472; ORF 521. ng>: g521n.pep 1 MKSKLPLILI NLSLISSPLG ANAAKIYTCT INGETVYTTK PSKSCHSTDL 51 PPIGNYSSER YILPQTPEPA PSPSNGGQAV KYKAPVKTVS KPAKSNTPPQ 101 QAPVNNSRRS ILEAELSNER KALTEAQKML SQARLAKGGN INHQKINAL* 151 SNVLDRQQNI QALQRELGRM * The following partial DNA sequence was identified in N meningitidis <SEQ ID 1473>: m521. seq 1 ATGAAATCAA AACTCCTCTT AATCCTAATC AACTTTTCCC TGATTTCAAG 51 CCCATTGGGT GCGAATGCGG CCAAAATCTA sACCTGCACA ATCAACGGAG 101 AAACCGTTTA CACCAsCAAG CCGTCCAAAA GCTGCCACTC AACCGATTTG 151 CCCCCAATCG GCAACTACAG CAGCGAACGC TATATCCCGC CCCAAACGCC 201 CGAACCGGTA TCATCACCGT CAAACGGCGG ACwGGTTGTC AAATATAAAG 251 CCCCGGTCAA AACAGTATCC AAGCCGGCAA AATCCArTAC GCCGCCGCCG 301 CAACAAGCAC CCTCAAACAA CAGCAGACGC TCCATTCTCG AAACAGAATT 351 GAGCAACGAA CGCAAAGCAT TGGTTGAAGC CCAAAAAATG TTATCACAAG 401 CACGTCTGGC AAAGGGCGGC AACATCAACC ATCAAGAAAT AAATGCATTA 451 CAAAGCAATG TATTGGACAG GCAGCAAAAT ATTCAAGCCC TGCAAAGGGA 501 ACTGGGGCGT ATGTAA This corresponds to the amino acid sequence <SEQ ID 1474; ORF 521>: m521.pep 1 MKSKLLLILI ΝFSLISSPLG AΝAAKIXTCT IΝGETVYTXK PSKSCHSTDL 51 PPIGNYSSER YIPPQTPEPV SSPSNGGXW KYKAPVKTVS KPAKSXTPPP 101 QQAPSNNSRR SILETELSNE RKALVEAQKM LSQARLAKGG NINHQEINAL 151 QSNVLDRQQN IQALQRELGR M* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 521 shows 90.6% identity over a 171 aa overlap with a predicted ORF (ORF 52 l.ng) from N gonorrhoeae: m521/g521 10 20 30 40 50 60 m521.pep MKSKLLLILIΝFSLISSPLGAΝAAKIXTCTIΝGETVYTXKPSKSCHSTDLPPIGΝYSSER I M M M M M M l M I I I I I I I I I I I M I I I I I I I I I I I I I I 1 1 I I M g521 MKSKLPLILIΝLSLISSPLGAΝAAKIYTCTIΝGETVYTTKPSKSCHSTDLPPIGΝYSSER 10 20 30 40 50 60 70 80 90 100 110 120 m521. pep YIPPQTPEPVSSPSΝGGXWKYKAPVKTVSKPAKSXTPPPQQAPSΝΝSRRSILETELSΝE I I I M M l l l l l l M M M M I M M M M I I I l l l l M M M M I M M M g521 YILPQTPEPAPSPSΝGGQAVKYKAPVKTVSKPAKSΝTPP-QQAPVΝΝSRRSILEAELSΝE 70 80 90 100 110 130 140 150 160 170 m521. pep RKALVEAQKMLSQARLAKGGΝIΝHQEIΝALQSΝVLDRQQΝIQALQRELGRMX MMMMMMIMMMMMMMIM MMMMMIMMMMM g521 RKALTEAQKMLSQARLAKGGΝIΝHQKIΝALXSΝVLDRQQΝIQALQRELGRMX 120 130 140 150 160 170 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1475>: a521. seq 1 ATGAAATCAA AACTCCCCTT AATCCTAATC AACTTTTCCC TGATTTCAAG 51 CCCATTGGGT GCGAATGCGG CCAAAATCTA CACCTGCACA ATCAACGGAG 101 AAACCGTTTA CACCACCAAG CCGTCCAAAA GCTGCCTCTC AACCGATTTG 151 CCCCCAATCG GCAACTACAG CAGCGAACGC TATATCCCGC CCCAAACATC 201 CGAACCGACA CCATCACCGT CAAACGGCGG ACAGGCTGTC AAATATAAAG 251 CCCCGGTCAA AACAGTATCC AAGCCGGCAA AATCCAATAC GCCGCCGCCG 301 CAACAAGCAC CCTCAAACAA CAGCAGACGC TCCATTCTCG AAACAGAATT 351 GAGCAACGAA CGCAAAGCAT TGGTTGAAGC CCAAAAAATG TTATCACAAG 401 CACGTCTGGC AAAAGGCGGC AACATCAACC ATCAAGAAAT CAACGCATTG 451 CAAAGCAATG TATTGGACAG GCAGCAAAAT ATCCAAGCAC TGCAAAGAGA 501 ATTGGGACGT ATGTAA This corresponds to the amino acid sequence <SEQ ID 1476; ORF 521. a>: a521.pep 1 MKSKLPLILI NFSLISSPLG ANAAKIYTCT INGETVYTTK PSKSCLSTDL 51 PPIGNYSSER YIPPQTSEPT PSPSNGGQAV KYKAPVKTVS KPAKSNTPPP 101 QQAPSNNSRR SILETELSNE RKALVEAQKM LSQARLAKGG NINHQEINAL 151 QSVLDRQQN IQALQRELGR M* m521 /a521 94.2% identity in 171 aa overlap 10 20 30 40 50 60 m521.pep MKSKLLLILINFSLISSPLGANAAKIXTCTINGETVYTXKPSKSCHSTDLPPIGNYSSER I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I llllllllllllll a521 MKSKLPLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKSCLSTDLPPIGNYSSER 10 20 30 40 50 60 70 80 90 100 110 120 m521.pep YIPPQTPEPVSSPSNGGXWKYKAPVKTVSKPAKSXTPPPQQAPSNNSRRSILETELSNE llllll II: llllll : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a521 YIPPQTSEPTPSPSNGGQAVKYKAPVKTVSKPAKSNTPPPQQAPSNNSRRSILETELSNE 70 80 90 100 110 120 130 140 150 160 170 m521. pep RKALVEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQNIQALQRELGRMX I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I a521 RKALVEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQNIQALQRELGRMX 130 140 150 160 170 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1477>: g522.seq 1 atgactgagc cgaaacacga aacgccgacg gaagagcagg ttgccgcgcg 51 caaaaaagca aaagccaaaa tccgcaccat ccgcatttgg gcgtgggtca 101 ttttggcgtt gctcgcttca accgccctgc tctcccaatg cgcgatgtcc 151 aaaccgcagg caaaacagaa aattgtcgag tcttgcatga aaaatattcc 201 gtttgctgaa aaatggcaga acgatttgaa agcgcgcggc ttggatgcgg 251 acaatacccg tctcgccgtc gactactgca aatgtatgtg ggagcagcct 301 ttggacggat tgagcgagaa acagatcagc tccttcggca aactcggtgc 351 acaagaacag cttgacctgc tcggcggcgc aaacgcgttt gaaactcgag 401 acaaacaatg tgtcgcggat ttgaaagccg attga This corresponds to the amino acid sequence <SEQ ID 1478; ORF 522.ng>: g52 . pep 1 MTEPKHETPT EEQVAARKKA KAKIRTIRIW AWVILALLAS TALLSQCAMS 51 KPQAKQKIVE SCMKΝIPFAE KWQΝDLKARG LDADΝTRLAV DYCKCMWEQP 101 LDGLSEKQIS SFGKLGAQEQ LDLLGGAΝAF ETRDKQCVAD LKAD* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1479>: m522. seq 1 ATGACTGAGC CGAAACACGA AATGCTGACG AAAGAGCAGG TTGCCGCGCG 51 CAAAAAAGCA AAAGCCAAAA TCCGCACCAT CCGCATTTGG GCGTGGGTCA 101 TTTTGGCGTT GCTCGCTTTA ACCGCCCTGC TCTCCCAATG CGCGATGTCC 151 AAACCGCAGG CAAAACAGAA AATTGTCGAG TCTTGCGTGA AGAATATTCC 201 GTTTGCCGAA AAATGGCAAA ACGATTTGCG GGCCCGCGGT TTAGATTCAA 251 ACAATACCCG CCTCGCCGTC GACTACTGCA AATGTATGTG GGAGCAGCCT 301 TTGGACAGAT TGAGCGAGAA ACAGATTAGA TCCTTCGGCA AACTCGGCGC 351 ACAAGAACAG CTTGACCTGC TCGGCGGCGC AAATGCCTTT GAAGCACGTG 401 ACAAGCAGTG TGTTGCCGAT TTGAAATCAG AATAA This corresponds to the amino acid sequence <SEQ ID 1480; ORF 522>: m522.pep 1 MTEPKHEMLT KEQVAARKKA KAKIRTIRIW AWVILALLAL TALLSQCAMS 51 KPQAKQKIVE SCVKNIPFAE KWQNDLRARG LDSNNTRLAV DYCKCMWEQP 101 LDRLSEKQIR SFGKLGAQEQ LDLLGGANAF EARDKQCVAD LKSE* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 522 shows 91.0% identity over a 144 aa overlap with a predicted ORF (ORF 522.ng) from N gonorrhoeae: m522/g522 10 20 30 40 50 60 m522.pep MTEPKHEMLTKEQVAARKKAKAKIRTIRIWAWVILALLALTALLSQCAMSKPQAKQKIVE IMMMMMMMMMIMMMMM MMMMMMMMMM g522 MTEPKHETPTEEQVAARKKAKAKIRTIRIWAWVILALLASTALLSQCAMSKPQAKQKIVE 10 20 30 40 50 60 70 80 90 100 110 120 m522.pep SCVKΝIPFAEKWQΝDLRARGLDSΝΝTRLAVDYCKCMWEQPLDRLSEKQIRSFGKLGAQEQ MMMMMMMMMMIhMMMMIMMMMM g522 SCMKΝIPFAEKWQΝDLKARGLDADΝTRLAVDYCKCMWEQPLDGLSEKQISSFGKLGAQEQ 70 80 90 100 110 120 130 140 m522.pep LDLLGGAΝAFEARDKQCVADLKSEX MMMMMIMMIMMM:: g522 LDLLGGAΝAFETRDKQCVADLKAD 130 140 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1481>: a522. seq 1 ATGACTGAGC CGAAACACGA AATGCCGACG GAAGAGCAGG TTGCCGCGCG 51 CAAAAAAGCA AAAGCCAAAA TCCGCACCAT CCGCATTTGG GCATGGGTCA 101 TTTTGGCGTT GCTCGCTTCA ACCGCCCTGC TCTCCCAATG CGCGATGTCC 151 AAACCGCAGG CAAAACAGAA AATTGTCGAG TCTTGCGTGA AGAATATTCC 201 GTTTGCCGAA AAATGGCAAA ACGATTTGCG GGCCCGCGGT TTAGATTCAA 251 ACAATACCCG CCTTACCGTC GACTACTGCA AATGTATGTG GGAGCAGCCT 301 TTGGACAGAT TGAGCGAGAA ACAGATTAGT TCCTTCGGCA AACTCGGCGC 351 ACAAGAACAG CTTGACCTGC TCGGCGGCGC AAATGCCTTT GAAACGCGAG 401 ACAAGCAGTG TGTTGCCGAT TTGAAATCAG AATAA This corresponds to the amino acid sequence <SEQ ID 1482; ORF 522.a>: a522.pep 1 MTEPKHEMPT EEQVAARKKA KAKIRTIRIW AWVILALLAS TALLSQCAMS 51 KPQAKQKIVE SCVKNIPFAE KWQNDLRARG LDSNNTRLTV DYCKCMWEQP 101 LDRLΞEKQIS SFGKLGAQEQ LDLLGGANAF ETRDKQCVAD LKSE* m522/a522 95.8% identity in 144 aa overlap 10 20 30 40 50 60 m522. pep MTEPKHEMLTKEQVAARKKAKAKIRTIRIWAWVILALLALTALLSQCAMSKPQAKQKIVE llllllll I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a522 MTEPKHEMPTEE Q VAARKKAKAKIRTIRIWAWVILALLASTALLSQCAMSKPQAKQKIVE 10 20 30 40 50 60 70 80 90 100 110 120 m522. pep SCVKNIPFAEKW Q NDLRARGLDSNNTRLAVDYCKCMWEQPLDRLSEKQIRSFGKLGAQEQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I: I I I I I I I I I I I I I I I M I I I llllllllll a522 SCVKNIPFAEKW Q NDLRARGLDSNNTRLTVDYCKCMWEQPLDRLSEKQISSFGKLGAQEQ 70 80 90 100 110 120 130 140 m522.pep LDLLGGANAFEARDKQCVADLKSEX I I I I I I I I I I I : I I I I I I I I I I I I I a522 LDLLGGANAFETRDKQCVADLKSEX 130 140 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 1483>: g523. seq 1 atgactgtat ggtttgttgc cgctgttgcc gtcttaatca tcgaattatt 51 gacgggaacg gtttatcttt tggttgtcag cgcggctttg gcgggttcgg 101 gcattgccta cgggctgact ggcagcacgc ctgccgccgt cttgaccgcc 151 gcactgcttt ccgcgctggg catttggttc gtacatgcca aaaccgccgt 201 gggaaaagtt gaaacggatt catatcagga tttggatacc ggaaaatatg 251 ccgaaatcct ccgatacaca ggcggcaacc gttacgaagt tttttatcgc 301 ggtacgcact ggcaggcgca aaatacgggg caggaagtgt ttgaaccggg 351 aacgcgcgcc ctcatcgtcc gcaaagaagg taaccttctt atcatcgcaa 401 acccttaa This corresponds to the amino acid sequence <SEQ ID 1484; ORF 523.ng>: g523 -pep 1 MTVWFVAAVA VLIIELLTGT VYLLWSAAL AGSGIAYGLT GSTPAAVLTA 51 ALLSALGIWF VHAKTAVGKV ETDSYQDLDT GKYAEILRYT GGΝRYEVFYR 101 GTHWQAQΝTG QEVFEPGTRA LIVRKEGΝLL IIAΝP* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1485>: m523.seq (partial) 1 ..GCCGTCTTAA TCATCGAATT ATTGACGGGA ACGGTTTATC TTTTGGTTGT 51 nAGCGCGGCT TTGGCGGGTT CGGGCATTGC TTACGGGCTG ACCGGCAGTA 101 CGCCTGCCGC CGTCTTGACC GnCGCTCTGC TTTCCGCGCT GGGTATTTnG 151 TTCGTACACG CCAAAACCGC CGTTAGAAAA GTTGAAACGG ATTCATATCA 201 GGATTTGGAT GCCGGACAAT ATGTCGAAAT CCTCCGACAC ACAGGCGGCA 251 ACCGTTACGA AGTTTTtTAT CGCGGTACGc ACTGGCAGGC TCAAAATACG 301 GGGCAAGAAG AGCTTGAACC AGGAACTCGC GCCCTCATTG TCCGCAAGGA 351 AGGCAACCTT CTTATTATCA CACACCCTTAA This corresponds to the amino acid sequence <SEQ ID 1486; ORF 523>: m523.pep (partial) 1 ..AVLIIELLTG TVYLLWSAA LAGSGIAYGL TGSTPAAVLT XALLSALGIX 51 FVHAKTAVRK VETDSYQDLD AGQYVEILRH TGGΝRYEVFY RGTHWQAQΝT 101 GQEELEPGTR ALIVRKEGΝL LIITHP* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF523 shows 91.3% identity over a 126 aa overlap with a predicted ORF (ORF 523.ng) from N gonorrhoeae: m523/g523 10 20 30 40 50 m523.pep AVLIIELLTGTVYLLWSAALAGSGIAYGLTGSTPAAVLTXALLSALGIXF MMIMMMMMMMMMIMMMMMMMM IIIIIMI I g523 MTVWFVAAVAVLIIELLTGTVYLLWSAALAGSGIAYGLTGSTPAAVLTAALLSALGIWF 10 20 30 40 50 60 60 70 80 90 100 110 m523. pep VHAKTAVRKVETDSYQDLDAGQYVEILRHTGGΝRYEVFYRGTHWQAQΝTGQEELEPGTRA Mlllll MMMMMIMMMMIMMMMMMMMMMMM MIIIM g523 VHAKTAVGKVETDSY Q DLDTGKYAEILRYTGGΝRYEVFYRGTHWQAQΝTGQEVFEPGTRA 70 80 90 100 110 120 120 m523.pep LIVRKEGΝLLIITHP g523 LIVRKEGNLLI IANPX 130 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1487>: a523. seq 1 ATGACTGTAT GGTTTGTTGC CGCTGTTGCC GTCTTAATCA TCGAATTATT 51 GACGGGAACG GTTTATCTTT TGGTTGTCAG CGCGGCTTTG GCGGGTTCGG 101 GCATTGCTTA CGGGCTGACC GGCAGCACGC CTGCCGCCGT CTTGACCGCC 151 GCTCTGCTTT CCGCGCTGGG TATTTGGTTC GTACACGCCA AAACCGCCGT 201 GGGAAAAGTT GAAACGGATT CATATCAGGA TTTGGATGCC GGGCAATATG 251 CCGAAATCCT CCGGCACGCA GGCGGCAACC GTTACGAAGT TTTTTATCGC 301 GGTACGCACT GGCAGGCTCA AAATACGGGG CAAGAAGAGC TTGAACCAGG 351 AACGCGCGCC CTAATCGTCC GCAAGGAAGG CAACCTTCTT ATCATCGCAA 401 AACCTTAA This corresponds to the amino acid sequence <SEQ ID 1488; ORF 523. a>: a523.pep 1 MTVWFVAAVA VLIIELLTGT VYLLWSAAL AGSGIAYGLT GSTPAAVLTA 51 ALLSALGIWF VHAKTAVGKV ETDSYQDLDA GQYAEILRHA GGΝRYEVFYR 101 GTHWQAQΝTG QEELEPGTRA LIVRKEGΝLL IIAKP* m523/a523 94.4% identity in 126 aa overlap 10 20 30 40 50 m523 . pep AVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTPAAVLTXALLSALGIXF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I l l l l l l l l I a523 MTVWFVAAVAVLIIELLTGTVYLLWSAALAGSGIAYGLTGSTPAAVLTAALLSALGIWF 10 20 30 40 50 60 60 70 80 90 100 110 m523. pep VHAKTAVRKVETDSYQDLDAGQYVEILRHTGGΝRYEVFYRGTHWQAQΝTGQEELEPGTRA lllllll I I I I I I I I I I I I I I I : I I I I I : I I I I I I I I I I II I I I I I I I I I I I I I I I I I I a523 VHAKTAVGKVETDSYQDLDAGQYAEILRHAGGΝRYEVFYRGTHWQAQΝTGQEELEPGTRA 70 80 90 100 110 120 120 m523.pep LIVRKEGNLLIITHPX I I I I I I I I I I I I :: I I a523 LIVRKEGNLLIIAKPX 130 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1489>: g525. seq 1 atgaagtacg tccggttatt tttcctcggc acggcactcg ccggcactca 51 agcggcggct gccgaaatgg ttcaaatcga aggcggcagc taccgcccgc 101 tttatctgaa aaaagatacc ggcctgatta aagtcaaacc gttcaaactg 151 gataaatatc ccgttaccaa tgccgagttt gccgaatttg tcaacagcca 201 cccccaatgg caaaaaggca ggatcggttc caaacaggca gaacccgctt 251 acctgaagca ttggatgaaa aacggcagcc gcagctatgc gccgaaggcg 301 ggcgaattga aacagccggt taccaatatt tcctggtttg ccgccaacgc 351 ctattgcgcc gcacaaggca aacgcctgcc gaccatcgac gaatgggaat 401 ttgccggact tgcttccgcc acgcagaaaa aacggctcaa acgaacccgg 451 ctacaaccgc actattctcg attggtatgc cgacggcgga cggaaaggcc 501 tgcacgatgt cggcaaagca ccgcccgaac tactggggtg tttatgatat 551 gcacgggctg a This corresponds to the amino acid sequence <SEQ ID 1490; ORF 525. ng>: g525.pep 1 MKYVRLFFLG TALAGTQAAA AEMVQIEGGS YRPLYLKKDT GLIKVKPFKL 51 DKYPVTΝAEF AEFVΝSHPQW QKGRIGSKQA EPAYLKHWMK ΝGSRSYAPKA 101 GELKQPVTΝI SWFAAΝAYCA AQGKRLPTID EWEFAGLASA TQKKRLKRTR 151 LQPHYSRLVC RRRTERPARC RQSTARTTGV FMICTG * The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1491>: m525.seq 1 ATGAAGTATG TCCGGTTATT TTwCCTCGGC GCGGCACTCG cCrrCACTCA 51 ArCGGCGGCT GcCGAAATGG TTCAAATCGA AGGCGGCAgC TACCGCCCrC 101 TTTATCTGAA AAAAGATACC GGCCTGATTA AAGTCAAACC GTTCAAACTG 151 GATAAATATC CCGTTACCAA TGCCGAGTTT GCCGAATTTG TCAACAGCCA 201 CCCCCAATGG CAAAAAGGCA GGATCGGTTC CAAACAGGCA GAACCCGCTT 251 ACCTGAAGCA TTGGATGAAA AACGGCAGCC GCAGCTATGc GCCGAAGgCG 301 GgCGAATTAA AACAACCGGT AACCAATGTT TCCTGGwTTG CCGCCAAcGC 351 CTAtTGCGCC GCACAAGGCA AACGCCTGCC GACCATTGAC GAATGGGAAT 401 TTGCCGGACT TGCTTCCGCC ACGCAGAAAA A.CGGCTCAA ACGAACCCGG 451 CTACAACCGC ACTATTCTCG ATTGGTATGC CGACGGCGGA CGGAAAGGCC 501 TGCACGATGT CGGCA.AAGG CCGCCCGAAC TACTGGGGCG TTTATGATAT 551 GCACGGGCTG A This corresponds to the amino acid sequence <SEQ ID 1492; ORF 525>: m525.pep 1 MKYVRLFXLG AALAXTQXAA AEMVQIEGGS YRPLYLKKDT GLIKVKPFKL 51 DKYPVTNAEF AEFVNSHPQW QKGRIGSKQA EPAYLKHWMK NGSRSYAPKA 101 GELKQPVTNV SWXAANAYCA AQGKRLPTID EWEFAGLASA TQKXRLKRTR 151 LQPHYSRLVC RRRTERPARC RXKAARTTGA FMICTG* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 525 shows 94.1% identity over a 186 aa overlap with a predicted ORF (ORF 525. ng) from N gonorrhoeae: m525/g525 10 20 30 40 50 60 m525.pep MKYVRLFXLGAALAXTQXAAAEMVQIEGGSYRPLYLKKDTGLIKVKPFKLDKYPVTΝAEF MMM II I II II II I II II II II II I II II II II II II I II I II I II II g525 MKYVRLFFLGTALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIKVKPFKLDKYPVTΝAEF 10 20 30 40 50 60 70 80 90 100 110 120 m525.pep AEFVΝSHPQWQKGRIGSKQAEPAYLKHWMKΝGSRSYAPKAGELKQPVTΝVSWXAAΝAYCA M M M M M M M M M M M M M M M M M M M M I M M M M M I M I I I M g525 AEFVΝSHPQWQKGRIGSKQAEPAYLKHWMKΝGSRSYAPKAGELKQPVTΝISWFAAΝAYCA 70 80 90 100 110 120 130 140 150 160 170 180 m525.pep AQGKRLPTIDEWEFAGLASATQKXRLKRTRLQPHYSRLVCRRRTERPARCRXKAARTTGA M M M I M M M M M M M M I I I I I I I I I I I I I I I I I I I I I I I I I I I : M I M h g525 AQGKRLPTIDEWEFAGLASATQKKRLKRTRLQPHYSRLVCRRRTERPARCRQSTARTTGV 130 140 150 160 170 180 m525.pep FMICTGX MIIIM g525 FMICTGX The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1493>: a525. seq 1 ATGAAGTTTA CCCGGTTACT CTTTCTCTGT GCGGCACTCG CCGGCACTCA 51 AGCGGCAGCT GCCGAAATGG TTCAAATCGA AGGCGGCAGC TACCGCCCGC 101 TTTATCTGAA AAAAGATACC GGCCTGATTA AAGTCAAACC GTTCAAACTG 151 GATAAATATC CCGTTACCAA TGCCGAGTTT GCCGAATTTG TCAACAGCCA 201 CCCCCAATGG CAAAAAGGCA GGATCGGTTC CAAACAGGCA GAACCCGCTT 251 ACCTGAAGCA TTGGATGAAA AACGGCAGCC GCAGCTATGC GCCGAAGGCG 301 GGCGATTTAA AACAACCGGT AACCAATGTT TCCTGGTTCG CCGCCAACGC 351 CTATTGCGCC GCACAAGGCA AACGCCTGCC GACCATTGAC GAATGGGAAT 401 TTGCCGGACT TGCCTCCGCC ACGCAG.AAA AACGGCTCAA ACGAACCCGG 451 CTACAACCGC ACTATTCTCG ACTGGTATGC GGATGGCGAC CGGAAAGACC 501 TGCACGATGT CGGCAAAG . G TCGCCCGAAC TACTGGGGCG TTTATGATAT 551 GCACGGTCTG A This corresponds to the amino acid sequence <SEQ ID 1494; ORF 525. a>: a525.pep 1 MKFTRLLFLC AALAGTQAAA AEMVQIEGGS YRPLYLKKDT GLIKVKPFKL 51 DKYPVTNAEF AEFVNSHPQW QKGRIGSKQA EPAYLKHWMK NGSRSYAPKA 101 GDLKQPVTNV SWFAANAYCA AQGKRLPTID EWEFAGLASA TQXKRLKRTR 151 LQPHYSRLVC GWRPERPARC RQXVARTTGA FMICTV* m525/a525 90.8% identity in 185 aa overlap 10 20 30 40 50 60 m525. pep MKYVRLFXLGAALAXTQXAAAEMVQIEGGSYRPLYLKKDTGLIKVKPFKLDKYPVTNAEF I I :: I I : I llll II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a525 MKFTRLLFLCAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIKVKPFKLDKYPVTNAEF 10 20 30 40 50 60 70 80 90 100 110 120 m525.pep AEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGELKQPVTNVSWXAANAYCA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I a525 AEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGDLKQPVTNVSWFAANAYCA 70 80 90 100 110 120 130 140 150 160 170 180 m525. pep AQGKRLPTIDEWEFAGLASATQKXRLKRTRLQPHYSRLVCRRRTERPARCRXKAARTTGA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I lllllll : I I I I I I a525 AQGKRLPTIDEWEFAGLASATQXKRLKRTRLQPHYSRLVCGWRPERPARCRQXVARTTGA 130 140 150 160 170 180 m525.pep FMICTGX I I I I I a525 FMICTVX The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1495>: g525-l.seq 1 ATGAAGTACG TCCGGTTATT TTTCCTCGGC ACGGCACTCG CCGGCACTCA 51 AGCGGCGGCT GCCGAAATGG TTCAAATCGA AGGCGGCAGC TACCGCCCGC 101 TTTATCTGAA AAAAGATACC GGCCTGATTA AAGTCAAACC GTTCAAACTG 151 GATAAATATC CCGTTACCAA TGCCGAGTTT GCCGAATTTG TCAACAGCCA 201 CCCCCAATGG CAAAAAGGCA GGATCGGTTC CAAACAGGCA GAACCCGCTT 251 ACCTGAAGCA TTGGATGAAA AACGGCAGCC GCAGCTATGC GCCGAAGGCG 301 GGCGAATTGA AACAGCCGGT TACCAATATT TCCTGGTTTG CCGCCAACGC 351 CTATTGCGCC GCACAAGGCA AACGCCTGCC GACCATCGAC GAATGGGAAT 401 TTGCCGGACT TGCTTCCGCC ACGCAGAAAA ACGGCTCAAA CGAACCCGGC 451 TACAACCGCA CTATTCTCGA TTGGTATGCC GACGGCGGAC GGAAAGGCCT 501 GCACGATGTC GGCAAAGACC GCCCGAACTA CTGGGGTGTT TATGATATGC 551 ACGGGCTGAT TTGGGAATGG ACGGAAGATT TCAACAGCAG CCTGCTTTCT 601 TCCGGCAATG CCAACGCGCA AATGTTTTGC AGCGGCGCAT CTGTCGGGGC 651 GAGCGACTCG TCCAACTATG CCGCCTTCCT CCGCTACGGC ATCCGCACCA 701 GCCTGCAATC CAAATACGTC CTGCACAACT TGGGCTTCCG CTGCGCAAGC 751 CGATAA This corresponds to the amino acid sequence <SEQ ID 1496; ORF 525-l.ng>: g525-l .pep 1 MKYVRLFFLG TALAGTQAAA AEMVQIEGGS YRPLYLKKDT GLIKVKPFKL 51 DKYPVTΝAEF AEFVΝSHPQW QKGRIGSKQA EPAYLKHWMK ΝGSRSYAPKA 101 GELKQPVTΝI SWFAANAYCA AQGKRLPTID EWEFAGLASA TQKNGSNEPG 151 YNRTILDWYA DGGRKGLHDV GKDRPNYWGV YDMHGLIWEW TEDFNSSLLS 201 SGNANAQMFC SGASVGASDS SNYAAFLRYG IRTSLQSKYV LHNLGFRCAS 251 R* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1497>: 525-l. seq 1 ATGAAGTATG TCCGGTTATT TTTCCTCGGC GCGGCACTCG CCGGCACTCA 51 AGCGGCGGCT GCCGAAATGG TTCAAATCGA AGGCGGCAGC TACCGCCCGC 101 TTTATCTGAA AAAAGATACC GGCCTGATTA AAGTCAAACC GTTCAAACTG 151 GATAAATATC CCGTTACCAA TGCCGAGTTT GCCGAATTTG TCAACAGCCA 201 CCCCCAATGG CAAAAAGGCA GGATCGGTTC CAAACAGGCA GAACCCGCTT 251 ACCTGAAGCA TTGGATGAAA AACGGCAGCC GCAGCTATGC GCCGAAGGCG 301 GGCGAATTAA AACAACCGGT AACCAATGTT TCCTGGTTTG CCGCCAACGC 351 CTATTGCGCC GCACAAGGCA AACGCCTGCC GACCATTGAC GAATGGGAAT 401 TTGCCGGACT TGCTTCCGCC ACGCAGAAAA ACGGCTCAAA CGAACCCGGC 451 TACAACCGCA CTATTCTCGA TTGGTATGCC GACGGCGGAC GGAAAGGCCT 501 GCACGATGTC GGCAAAGGCC GCCCGAACTA CTGGGGCGTT TATGATATGC 551 ACGGGCTGAT TTGGGAATGG ACGGAAGATT TCAACAGCAG CCTGCTTTCT 601 TCCGGCAATG CCAACGCGCA AATGTTTTGC AGCGGCGCGT CTATCGGGTC 651 GAGCGACTCG TCCAACTATG CCGCCTTCCT CCGCTACGGC ATCCGTACCA 701 GCCTGCAATC CAAATATGTC TTGCACAACT TGGGCTTCCG TTGCACAAGC 751 CGATAA This corresponds to the amino acid sequence <SEQ ID 1498; ORF 525-l>: m525-l .pep 1 MKYVRLFFLG AALAGTQAAA AEMVQIEGGS YRPLYLKKDT GLIKVKPFKL 51 DKYPVTNAEF AEFVNSHPQW QKGRIGSKQA EPAYLKHWMK NGSRSYAPKA 101 GELKQPVTNV SWFAANAYCA AQGKRLPTID EWEFAGLASA TQKNGSNEPG 151 YNRTILDWYA DGGRKGLHDV GKGRPNYWGV YDMHGLIWEW TEDFNSSLLS 201 SGNANAQMFC SGASIGSSDS SNYAAFLRYG IRTSLQSKYV LHNLGFRCTS 251 R* m525-l/g525-l 97.6% identity in 251 aa overlap 10 20 30 40 50 60 m525-l.pep MKYVRLFFLGAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIKVKPFKLDKYPVTNAEF I I I I I I I I I hi I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g525-l MKYVRLFFLGTALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIKVKPFKLDKYPVTNAEF 10 20 30 40 50 60 70 80 90 100 110 120 m525-l . pep AEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGELKQPVTNVSWFAANAYCA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I g525-l AEFVNSHPQWQKGRIGΞKQAEPAYLKHWMKNGSRSYAPKAGELKQPVTNISWFAANAYCA 70 80 90 100 110 120 130 140 150 160 170 180 m525-l . pep AQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYADGGRKGLHDVGKGRPNYWGV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g525-l AQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYADGGRKGLHDVGKDRPNYWGV 130 140 150 160 170 180 190 200 210 220 230 240 m525-l.pep YDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASIGSSDSΞNYAAFLRYGIRTSLQSKYV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I hhl I I I I I I I I I I I I I I I I I I I I I I g525-l YDMHGLIWEWTEDFNSΞLLSSGNANAQMFCSGASVGASDSSNYAAFLRYGIRTSLQSKYV 190 200 210 220 230 240 250 m525- -1. pep LHNLGFRCTSRX hill g525' -1 LHNLGFRCASRX 250 The following partial DNA sequence was :identified in N menin\ a525 -l.seq 1 ATGAAGTTTA CCCGGTTACT CTTTCTCTGT GCGGCACTCG CCGGCACTCA 51 AGCGGCAGCT GCCGAAATGG TTCAAATCGA AGGCGGCAGC TACCGCCCGC 101 TTTATCTGAA AAAAGATACC GGCCTGATTA AAGTCAAACC GTTCAAACTG 151 GATAAATATC CCGTTACCAA TGCCGAGTTT GCCGAATTTG TCAACAGCCA 201 CCCCCAATGG CAAAAAGGCA GGATCGGTTC CAAACAGGCA GAACCCGCTT 251 ACCTGAAGCA TTGGATGAAA AACGGCAGCC GCAGCTATGC GCCGAAGGCG 301 GGCGATTTAA AACAACCGGT AACCAATGTT TCCTGGTTCG CCGCCAACGC 351 CTATTGCGCC GCACAAGGCA AACGCCTGCC GACCATTGAC GAATGGGAAT 401 TTGCCGGACT TGCCTCCGCC ACGCAGAAAA ACGGCTCAAA CGAACCCGGC 451 TACAACCGCA CTATTCTCGA CTGGTATGCG GATGGCGACC GGAAAGACCT 501 GCACGATGTC GGCAAAGGTC GCCCGAACTA CTGGGGCGTT TATGATATGC 551 ACGGTCTGAT TTGGGAATGG ACGGAAGATT TCAACAGCAG CCTGCTTTCT 601 TCCGGCAATG CCAACGCGCA AATGTTTTGC AGCGGCGCGT CTATCGGGTC 651 GAGCGACTCG TCCAACTATG CCGCCTTCCT CCGCTACGGC ATCCGCACCA 701 GCCTGCAATC CAAATATGTC TTGCACAACT TGGGCTTCCG TTGCACAAGC 751 CGATAA This corresponds to the amino acid sequence <SEQ ID 1500; ORF 525-1. a>: a525-l.pep 1 MKFTRLLFLC AALAGTQAAA AEMVQIEGGS YRPLYLKKDT GLIKVKPFKL 51 DKYPVTNAEF AEFVNSHPQW QKGRIGSKQA EPAYLKHWMK NGSRSYAPKA 101 GDLKQPVTNV SWFAANAYCA AQGKRLPTID EWEFAGLASA TQKNGSNEPG 151 YNRTILDWYA DGDRKDLHDV GKGRPNYWGV YDMHGLIWEW TEDFNSSLLS 201 SGNANAQMFC SGASIGΞSDS SNYAAFLRYG IRTSLQSKYV LHNLGFRCTS 251 R* m525-l/a525-l 97.2% identity in 251 aa overlap 10 20 30 40 50 60 m525-l.pep MKYVRLFFLGAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIKVKPFKLDKYPVTNAEF I I :: I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a525-l MKFTRLLFLCAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIKVKPFKLDKYPVTNAEF 10 20 30 40 50 60 70 80 90 100 110 120 m525-l.pep AEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGELKQPVTNVSWFAANAYCA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I :| I I I I I I I I I I I I I I I I I a525-l AEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRΞYAPKAGDLKQPVTNVSWFAANAYCA 70 80 90 100 110 120 130 140 150 160 170 180 m525-l.pep AQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYADGGRKGLHDVGKGRPNYWGV I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I II llllllllllllll a525-l AQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYADGDRKDLHDVGKGRPNYWGV 130 140 150 160 170 180 190 200 210 220 230 240 m525-l.pep YDMHGLIWEWTEDFNSSLLSSGNANAQMFCΞGASIGSΞDSSNYAAFLRYGIRTSLQΞKYV I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I II I I I I I I I I I I I a525-l YDMHGLIWEWTEDFNSΞLLSΞGNANAQMFCSGASIGSSDSSNYAAFLRYGIRTSLQSKYV 190 200 210 220 230 240 250 m525-l.pep LHNLGFRCTSRX I I I I I I I I I I I I a525-l LHNLGFRCTSRX 250 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1501>: g527.seq 1 atggttttac cagtctcctt ttttcagcct gtccagttgg cggcggtcgc 51 gcttggtcgg tctgccgtcg ggatgggcgg aagtgatgcg gctgaattgg 101 tcgagctgtt tgcactcttc cctcaatgct gccgttttcg cgtcttcttc 151 atacagaagc cgcgcctcgg gtgccgggcg gcgttggtgg ttcaaacctt 201 taaccttgat tttatgggga agggaattga gcgtcaggtc gataatatcg 251 ccgatgtcta tggttttact gtttttgact ttcgagccgt ttacttgaac 301 cctacccagt tcgatatgct tttgcgcaag ggaacgggtc ttgaaaaaac 351 gtgccgccca aagccatttg tccagccgca tggcggaaga atcgtgcttg 401 tctttcatac gattttgttt gaaataattg aatttgtttc gagtttagca 451 taa This corresponds to the amino acid sequence <SEQ ID 1502; ORF 527.ng>: g527.pep 1 MVLPVSFFQP VQLAAVALGR SAVGMGGSDA AELVELFALF PQCCRFRVFF 51 IQKPRLGCRA ALWQTFΝLD FMGKGIERQV DΝIADVYGFT VFDFRAVYLΝ 101 PTQFDMLLRK GTGLEKTCRP KPFVQPHGGR IVLVFHTILF EIIEFVSSLA 151 * The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1503>: m527. seq 1 ATGGTTTTAC CAGTCTCCTT TTTTCAGCCT GTCCAGTTGG CGGCGGTCGC 51 GCTTGGTCGG TCTGCCGTCG GGATAGGCGG AAGTGATGCG GCTGAATTGG 101 TCGAGCTGTT TGCGCTCTTC CCTCAATGTT GCCGTT TCG CGTCCTCTTC 151 ATACAGAAGC CGCGCyTCGG ATGCCGGGCG GCGTTGGTGG TTCAAACCTT 201 TAACCkTGAT TTTATAGGGA AGGG.AATTk AgCkTCaGTy GrT ATaTCG 251 CsGATGTmTA TGGTTTTACT GTTTTTGACC TTCGAGCCGT TTACTTGAAC 301 CCTACCCAGT TCGATGTGCT TTTGCGCAAG GGAACGGGTC TTGAAAAAAC 351 GTGCCGCCCA AAGCCATTTG TCCAGCCGCA TGGCGGAAGA ATCGTGCTTG 401 TCTTTCATAC GATTTTGTTT GAAATAATTG AATTTGTTTC GAGTTTAGCA 451 TAA This corresponds to the amino acid sequence <SEQ ID 1504; ORF 527>: m527pep 1 MVLPVSFFQP VQLAAVALGR SAVGIGGSDA AELVELFALF PQCCRXRVLF 51 IQKPRXGCRA ALWQTFNXD FIGKXNXASV XXIADVYGFT VFDLRAVYLN 101 PTQFDVLLRK GTGLEKTCRP KPFVQPHGGR IVLVFHTILF EIIEFVSSLA 151 * Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 527 shows 90.0% identity over a 150 aa overlap with a predicted ORF (ORF 527.ng) from N gonorrhoeae: m527/g527 10 20 30 40 50 60 m527. pep MVLPVSFFQPVQLAAVALGRSAVGIGGSDAAELVELFALFPQCCRXRVLFIQKPRXGCRA M M M M M M MM MM M MMM M MMMI M MM M I IM I I I M M M g527 MVLPVSFFQPVQLAAVALGRSAVGMGGSDAAELVELFALFPQCCRFRVFFIQKPRLGCRA 10 20 30 40 50 60 70 80 90 100 110 120 m527. pep ALWQTFΝXDFIGKXΝXASVXXIADVYGFTVFDLRAVYLΝPTQFDVLLRKGTGLEKTCRP II II IM I Ml MMM MM IM MMMM MMM M I M M M M g527 ALWQTFΝLDFMGKGIERQVDΝIADVYGFTVFDFRAVYLΝPTQFDMLLRKGTGLEKTCRP 70 80 90 100 110 120 130 140 150 m527.pep KPFVQPHGGRIVLVFHTILFEIIEFVSSLA MMMMMMMMMMMMMMM g527 KPFVQPHGGRIVLVFHTILFEIIEFVSSLA 130 140 150 The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 1505>: a527. seq 1 ATGGTTTTAC CAGTCTCCTT TTTTCAGCCT GTCCAGTTGG CGGCGGTCGC 51 GCTTGGTCGG TCTGCCGTCG GGATAGGCGG AAGTGATGCG GCTGAATTGG 101 TCGAGCTGTT TGCGCTCTTC CCTCAATGTT GCCGTTTTCG CGTCCTCTTC 151 ATACAGAAGC CGCGCCTCGG ATGCCGGGCG GCGTTGGTGG TTCAAACCTT 201 TAACCTTGAT TTTATAGGGA AGGGAATTGA GCGTCAGGTC GATAATATCG 251 CCGATGTCTA TGGTTTTACT GTTTTTGACC TTCGAGCCGT TTACTTGAAC 301 CCTACCCAGT TCGATGTGCT TTTGCGCAAG GGAACGGGTC TTGAAAAAAC 351 GTGCCGCCCA AAGCCATTTG TCCAGCCGCA TGGCGGAAGA ATCGTGCTTG 401 TCTTTCATAC GATTTTGTTT GAAATAATTG AATTTGTTTC GAGTTTAGCA 451 TAA This corresponds to the amino acid sequence <SEQ ID 1506; ORF 527. a>: a527.pep 1 MVLPVSFFQP VQLAAVALGR SAVGIGGSDA AELVELFALF PQCCRFRVLF 51 IQKPRLGCRA ALWQTFΝLD FIGKGIERQV DΝIADVYGFT VFDLRAVYLN 101 PTQFDVLLRK GTGLEKTCRP KPFVQPHGGR IVLVFHTILF EIIEFVSSLA 151 * m527/a527 93.3% identity in 150 aa overlap 10 20 30 40 50 60 m527. pep MVLPVSFF Q PV Q LAAVALGRSAVGIGGSDAAELVELFALFPQCCRXRVLFIQKPRXGCRA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I lllllllll llll a527 MVLPVSFF Q PV Q LAAVALGRSAVGIGGSDAAELVELFALFPQCCRFRVLFIQKPRLGCRA 10 20 30 40 50 60 70 80 90 100 110 120 m527. pep ALVVQTFNXDFIGKXNXASVXXIADVYGFTVFDLRAVYLNPTQFDVLLRKGTGLEKTCRP llllllll I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a527 ALVVQTFNLDFIGKGIERQVDNIADVYGFTVFDLRAVYLNPTQFDVLLRKGTGLEKTCRP 70 80 90 100 110 120 130 140 150 m52 .pep KPFVQPHGGRIVLVFHTILFEIIEFVSSLAX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a527 KPFVQPHGGRIVLVFHTILFEIIEFVSSLAX 130 140 150 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1507>: g528.seq 1 atggaaattc gggtaataaa atatacggca acggctgcgt tgtttgcatt 51 tacggttgca ggctgccggc tggcggggtg gtatgagtgt ttgtccttgt 101 ccggctggtg taagccgaga aaacctgccg ccatcgattt ttgggatatt 151 ggcggcgaga gtccgctgtc tttagaggac tacgagatac cgctttcaga 201 cggcaatcgt tccgtcaggg caaacgaata tgaatccgcg caaaaatctt 251 acttttatag gaaaataggg aagtttgaag cctgcgggtt ggattggcgt 301 acgcgtgacg gcaaaccttt ggttgagagg ttcaaacagg aaggtttcga 351 ctgtttggaa aagcaggggt tgcggcgcaa cggcctgtcc gagcgcgtcc 401 gatggtaa This corresponds to the amino acid sequence <SEQ ID 1508; ORF 528.ng>: g528.pep 1 MEIRVIKYTA TAALFAFTVA GCRLAGWYEC LSLSGWCKPR KPAAIDFWDI 51 GGESPLSLED YEIPLSDGΝR SVRAΝEYESA QKSYFYRKIG KFEACGLDWR 101 TRDGKPLVER FKQEGFDCLE KQGLRRΝGLS ERVRW* The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 1509>: m528.seq (partial) 1 ATGGAAATTC GGGCAATAAA ATATACGGCA ATGGCTGCGT TGCTTGCATT 51 TACGGTTGCA GGCTGCCGGC TGGCGGGGTG GTATGAGTGT TCGTCCCTCA 101 CCGGCTGGTG TAAGCCGAGA AAACCGGCTG CCATCGATTT TTGGGATATT 151 GGCGGCGAGA GTCCGCCGTC TTTAGGGGAC TACGAGATAC CGCTTTCAGA 201 CGGCAATAGT TCCGTCAGGG CAAACGAATA TGAATCCGCA CAACAATCTT 251 ACTTTTACAG GAAAATAGGG AAGTTTGAAG C . TGCGGGCT GGATTGGCGT 301 ACGCGTGACG GCAAACCTTT GATTGAGACG TTCAAACAGG GAGGATTTGA 351 CTGCTTGGAA AAG.... This corresponds to the amino acid sequence <SEQ ID 1510; ORF 528>: m528.pep (partial) 1 MEIRAIKYTA MAALLAFTVA GCRLAGWYEC SSLTGWCKPR KPAAIDFWDI 51 GGESPPSLGD YEIPLSDGΝS SVRAΝEYESA QQSYFYRKIG KFEXCGLDWR 101 TRDGKPLIET FKQGGFDCLE K.... Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 528 shows 89.3% identity over a 121 aa overlap with a predicted ORF (ORF 528.ng) from N gonorrhoeae: m528/g528 10 20 30 40 50 60 m528. .pep MEIRAIKYTAMAALLAFTVAGCRLAGWYECSSLTGWCKPRKPAAIDFWDIGGESPPSLGD lllhlllll I I I : I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I II I g528 MEIRVIKYTATAALFAFTVAGCRLAGWYECLSLSGWCKPRKPAAIDFWDIGGESPLSLED 10 20 30 40 50 60 70 80 90 100 110 120 m528. .pep YEIPLSDGΝSSVRAΝEYESAQQSYFYRKIGKFEXCGLDWRTRDGKPLIETFKQGGFDCLE lllllllll I I I I I I I I I I I: I I I I I I I I I I I I I I I I I I I I I I I I: I III llllll g528 YEIPLSDGΝRSVRAΝEYESAQKSYFYRKIGKFEACGLDWRTRDGKPLVERFKQEGFDCLE 70 80 90 100 110 120 m528.pep K I g528 KQGLRRNGLSERVRW The following partial DNA sequence was identified in N meningitidis <SEQ ID 1511>: a528. seq 1 ATGGAAATTC GGGCAATAAA ATATACGGCA ATGGCTGCGT TGCTTGCATT 51 TACGGTTGCA GGCTGCCGGT TGGCAGGTTG GTATGAGTGT TCGTCCCTGT 101 CCGGCTGGTG TAAGCCGAGA AAACCTGCCG CCATCGATTT TTGGGATATT 151 GGCGGCGAGA GTCCTCCGTC TTTAGAGGAC TACGAGATAC CGCTTTCAGA 201 CGGCAATCGT TCCGTCAGGG CAAACGAATA TGAATCCGCA CAACAATCTT 251 ACTTTTACAG GAAAATAGGG AAGTTTGAAG CCTGCGGGTT GGATTGGCGT 301 ACGCGTGACG GCAAACCTTT GATTGAGACG TTCAAACAGG AAGGTTTTGA 351 TTGTTTGAAA AAGCAGGGGT TGCGGCGCAA CGGTCTGTCC GAGCGCGTCC 401 GATGGTAA This corresponds to the amino acid sequence <SEQ ID 1512; ORF 528. a>: a528.pep 1 MEIRAIKYTA MAALLAFTVA GCRLAGWYEC SSLSGWCKPR KPAAIDFWDI 51 GGESPPSLED YEIPLSDGΝR SVRAΝEYESA QQSYFYRKIG KFEACGLDWR 101 TRDGKPLIET FKQEGFDCLK KQGLRRΝGLS ERVRW* m528/a528 95.0% identity in 121 aa overlap 10 20 30 40 50 60 m528.pep MEIRAIKYTAMAALLAFTVAGCRLAGWYECSSLTGWCKPRKPAAIDFWDIGGESPPSLGD I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I a528 MEIRAIKYTAMAALLAFTVAGCRLAGWYECSSLSGWCKPRKPAAIDFWDIGGESPPSLED 10 20 30 40 50 60 70 80 90 100 110 120 m528.pep YEIPLSDGΝSSVRAΝEYESAQQSYFYRKIGKFEXCGLDWRTRDGKPLIETFKQGGFDCLE lllllllll I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I Mill: a528 YEIPLSDGΝRSVRAΝEYESAQQSYFYRKIGKFEACGLDWRTRDGKPLIETFKQEGFDCLK 70 80 90 100 110 120 m528.pep K I a528 KQGLRRΝGLSERVRWX 130 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 1513>: g528-l.seq 1 ATGGAAATTC GGGTAATAAA ATATACGGCA ACGGCTGCGT TGTTTGCATT 51 TACGGTTGCA GGCTGCCGGC TGGCGGGGTG GTATGAGTGT TCGTCCTTGT 101 CCGGCTGGTG TAAGCCGAGA AAACCTGCCG CCATCGATTT TTGGGATATT 151 GGCGGCGAGA GTCCGCTGTC TTTAGAGGAC TACGAGATAC CGCTTTCAGA 201 CGGCAATCGT TCCGTCAGGG CAAACGAATA TGAATCCGCG CAAAAATCTT 251 ACTTTTATAG GAAAATAGGG AAGTTTGAAG CCTGCGGGTT GGATTGGCGT 301 ACGCGTGACG GCAAACCTTT GGTTGAGAGG TTCAAACAGG AAGGTTTCGA 351 CTGTTTGGAA AAGCAGGGGT TGCGGCGCAA CGGCCTGTCC GAGCGCGTCC 401 GATGGTAA This corresponds to the amino acid sequence <SEQ ID 1514; ORF 528-l.ng>: g528-l.pep 1 MEIRVIKYTA TAALFAFTVA GCRLAGWYEC SSLSGWCKPR KPAAIDFWDI 51 GGESPLSLED YEIPLΞDGΝR SVRAΝEYESA QKSYFYRKIG KFEACGLDWR 101 TRDGKPLVER FKQEGFDCLE KQGLRRΝGLS ERVRW* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1515>: m528-l.seq 1 ATGGAAATTC GGGCAATAAA ATATACGGCA ATGGCTGCGT TGCTTGCATT 51 TACGGTTGCA GGCTGCCGGC TGGCGGGGTG GTATGAGTGT TCGTCCCTCA 101 CCGGCTGGTG TAAGCCGAGA AAACCGGCTG CCATCGATTT TTGGGATATT 151 GGCGGCGAGA GTCCGCCGTC TTTAGGGGAC TACGAGATAC CGCTTTCAGA 201 CGGCAATCGT TCCGTCAGGG CAAACGAATA TGAATCCGCA CAACAATCTT 251 ACTTTTACAG GAAAATAGGG AAGTTTGAAG CCTGCGGGCT GGATTGGCGT 301 ACGCGTGACG GCAAACCTTT GATTGAGACG TTCAAACAGG GAGGATTTGA 351 CTGCTTGGAA AAGCAGGGGT TGCGGCGCAA CGGTCTGTCC GAGCGCGTCC 401 GATGGTAA This corresponds to the amino acid sequence <SEQ ID 1516; ORF 528-l>: m528-l.pep.. 1 MEIRAIKYTA MAALLAFTVA GCRLAGWYEC SSLTGWCKPR KPAAIDFWDI 51 GGESPPSLGD YEIPLSDGNR SVRANEYESA QQSYFYRKIG KFEACGLDWR 101 TRDGKPLIET FKQGGFDCLE KQGLRRNGLS ERVRW* g528-l/m528-l 92.6% identity in 135 aa overlap 10 20 30 40 50 60 g528-l.pep MEIRVIKYTATAALFAFTVAGCRLAGWYECΞΞLSGWCKPRKPAAIDFWDIGGESPLSLED lllhlllll I I hi I I I I I I I I I I I I I I I I hi I I I I I I I I I I I I I I I I I I I I II I m528-l MEIRAIKYTAMAALLAFTVAGCRLAGWYECSSLTGWCKPRKPAAIDFWDIGGESPPSLGD 10 20 30 40 50 60 70 80 90 100 110 120 g528-l.pep YEIPLSDGNRSVRANEYESAQKSYFYRKIGKFEACGLDWRTRDGKPLVERFKQEGFDCLE I I I I I I I I I I I I I I I I I I I I h I I I I I I I I I I I I I I I I I I I I I I I I I: I III llllll m528-l YEIPLSDGNRSVRANEYESAQQSYFYRKIGKFEACGLDWRTRDGKPLIETFKQGGFDCLE 70 80 90 100 110 120 130 g528-l .pep KQGLRRNGLSERVRWX I I I I I I I I I I I I I I I I m528-l KQGLRRNGLSERVRWX 130 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1517>: a528-l.seq 1 ATGGAAATTC GGGCAATAAA ATATACGGCA ATGGCTGCGT TGCTTGCATT 51 TACGGTTGCA GGCTGCCGGT TGGCAGGTTG GTATGAGTGT TCGTCCCTGT 101 CCGGCTGGTG TAAGCCGAGA AAACCTGCCG CCATCGATTT TTGGGATATT 151 GGCGGCGAGA GTCCTCCGTC TTTAGAGGAC TACGAGATAC CGCTTTCAGA 201 CGGCAATCGT TCCGTCAGGG CAAACGAATA TGAATCCGCA CAACAATCTT 251 ACTTTTACAG GAAAATAGGG AAGTTTGAAG CCTGCGGGTT GGATTGGCGT 301 ACGCGTGACG GCAAACCTTT GATTGAGACG TTCAAACAGG AAGGTTTTGA 351 TTGTTTGAAA AAGCAGGGGT TGCGGCGCAA CGGTCTGTCC GAGCGCGTCC 401 GATGGTAA This corresponds to the amino acid sequence <SEQ ID 1518; ORF 528-1. a>: a528-l.pep 1 MEIRAIKYTA MAALLAFTVA GCRLAGWYEC SSLSGWCKPR KPAAIDFWDI 51 GGESPPSLED YEIPLSDGNR SVRANEYESA QQSYFYRKIG KFEACGLDWR 101 TRDGKPLIET FKQEGFDCLK KQGLRRNGLS ERVRW* a528-l/m528-l 97.0% identity in 135 aa overlap 10 20 30 40 50 60 a528-l.pep MEIRAIKYTAMAALLAFTVAGCRLAGWYECΞΞLΞGWCKPRKPAAIDFWDIGGESPPSLED I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I hi I I I I I I I I I I I I I I I I I I I I I I I I m528-l MEIRAIKYTAMAALLAFTVAGCRLAGWYECΞSLTGWCKPRKPAAIDFWDIGGESPPSLGD 10 20 30 40 50 60 70 80 90 100 110 120 a528-l.pep YEIPLSDGNRSVRANEYESAQQSYFYRKIGKFEACGLDWRTRDGKPLIETFKQEGFDCLK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I Mill: m528-l YEIPLSDGNRΞVRANEYESAQQSYFYRKIGKFEACGLDWRTRDGKPLIETFKQGGFDCLE 70 80 90 100 110 120 130 a528-l.pep KQGLRRNGLSERVRWX I I I I I I I I I I I I I I I I m528-l KQGLRRNGLSERVRWX 130 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1519>: g529.seq (partial) 1 atgacccata tcaaacccgt cattgccgcg ctcgcactca tcgggcttgc 51 cgcctgctcc ggcagcaaaa ccgaacagcc caagctcgac taccaaagcc 101 ggtcgcaccg cctgatcaaa ctcgaagtcc cgcctgattt gaacaacccc 151 gaccaaggca acctctaccg cctgcctgcc ggttcgggag ccgtccgcgc 201 cggggatttg gaaaaacgcc gcacacccgc cgtccaacag ccagcggatg 251 ccggaagtat tgaaaagcgt caaaggcgtc cgcttcgagc ggcgacggca 301 gccaacgcct ggcttgtcgt tgacggcaaa tcccccgccg aaatctccgc 351 cgctttctg. This corresponds to the amino acid sequence <SEQ ID 1520; ORF 529.ng>: g529.pep (partial) 1 MTHIKPVIAA LALIGLAACS GSKTEQPKLD YQSRSHRLIK LEVPPDLΝΝP 51 DQGΝLYRLPA GSGAVRAGDL EKRRTPAVQQ PADAGSIEKR QRRPLRAATA 101 AΝAWLWDGK SPAEISAAF.. The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1521>: m529. seq 1 ATGACCCATA TCAAACCCGT CATTGCCGCG CTCGCACTCA TCGGGCTTGC 51 CGCCTGCTCC GGCAGCAAAA CCGAACAGCC CAAGCTCGAC TACCAAAGCC 101 GGTCGCACCG CCTGATCAAA CTTGAAGTCC CACCTGATTT GAACAACCCC 151 GACCAAGGCA ACCTCTACCG CCTGCCTGCC GGTTCGGGCG CCGTCCGCGC 201 CAGCGATTTG GAAAAACGCC GCACACCCGC CGTCCAACAG CCTGCCGATG 251 CCGAAGTATT GAAAAGCGTC AAAGGTGTCC GCCTCGAGCG CGACGGCAGC 301 CAACGCTGGC TCGTTGTCGA CGGCAAGTCT CCTGCCGAAA TCTGGCCGCT 351 CCTGAAAGCC TTTTGGCAGG AAAACGGCTT CGACATCAAA TCCGAAGAAC 401 CCGCCATCGG ACAAATGGAA ACCGAGTGGG CGGAAAACCG CGCCAAAATC 451 CCCCAAGACA GCTTGCGCCG CCTCTTCGAC AAAGTCGGCT TGGGCGGCAT 501 CTACTCCACC GGCGAGCGCG ACAAATTCAT CGTCCGTATC GAACAGGGCA 551 AAAACGGCGT TTCCGACATC TTCTTCGCCC ACAAAGCCAT GAAAGAAGTG 601 TACGGCGGCA AAGACAAAGA CACGACCGTA TGGCAGCCCT CCCCGTCCGA 651 TCCCAACCTC GAAGCCGCTT TCCTGACGCG CTTTATGCAA TATTTGGGCG 701 TTGACGGACA GCAGGCGGAA AACGCATCGG CAAAAAAACC TACCCTTCCC 751 GCCGCCAACG AAATGGCGCG TATCGAAGGC AAAAGCCTGA TTGTCTTTGG 801 CGACTACGGC AGAAACTGGC GGCGCACCGT GCTCGCCCTC GACCGCATCG 851 GGCTGACCGT CGTCGGTCAA AACACCGAAC GCCACGCCTT CCTGGTTCAA 901 AAAGCCCCGA ACGAAAGCAA TGCAGTTACC GAACAAAAAC CCGGCCTGTT 951 CAAACGCCTG CTGGGCAAAG GCAAAGCGGA GAAACCTGCC GAACAGCCGG 1001 AACTGATTGT CTATGCAGAA CCTGTCGCCA ACGGCTCGCG CATCGTCCTG 1051 CTCAACAAAG ACGGCAGCGC ATATGCCGGC AAAGACGCAT CCGCATTATT 1101 GGGCAAACTC CATTCCGAAC TGCGTTAA This corresponds to the amino acid sequence <SEQ ID 1522; ORF 529>: m529.pep 1 MTHIKPVIAA LALIGLAACS GSKTEQPKLD YQSRSHRLIK LEVPPDLΝΝP 51 DQGΝLYRLPA GSGAVRASDL EKRRTPAVQQ PADAEVLKSV KGVRLERDGS 101 QRWLWDGKS PAEIWPLLKA FWQEΝGFDIK SEEPAIGQME TEWAEΝRAKI 151 PQDSLRRLFD KVGLGGIYST GERDKFIVRI EQGKΝGVSDI FFAHKAMKEV 201 YGGKDKDTTV WQPSPSDPΝL EAAFLTRFMQ YLGVDGQQAE ΝASAKKPTLP 251 AAΝEMARIEG KSLIVFGDYG RΝWRRTVLAL DRIGLTWGQ ΝTERHAFLVQ 301 KAPΝESΝAVT EQKPGLFKRL LGKGKAEKPA EQPELIVYAE PVAΝGSRIVL 351 LΝKDGSAYAG KDASALLGKL HSELR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 529 shows 83.5% identity over a 115 aa overlap with a predicted ORF (ORF 529.ng) from N gonorrhoeae: g529/m529 10 20 30 40 50 60 g529.pep MTHIKPVIAALALIGLAACSGSKTEQPKLDYQSRSHRLIKLEVPPDLΝΝPDQGΝLYRLPA m529 MTHIKPVIAALALIGLAACSGSKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPA 10 20 30 40 50 60 70 80 90 100 110 120 g529.pep GSGAVRAGDLEKRRTPAVQQPADAGSIEKRQRRPLRAATAANAWLWDGKSPAEISAAFX M I M M M M M M I M M M M - = h ■■ ■■ ■■ 1 1 I I 1 1 1 1 1 1 1 1 m529 GSGAVRASDLEKRRTPAVQQPADAEVLKSVKGVRLER-DGSQRWLWDGKSPAEIWPLLK 70 80 90 100 110 m529 AFWQENGFDIKSEEPAIGQMETEWAENRAKIPQDSLRRLFDKVGLGGIYSTGERDKFIVR 120 130 140 150 160 170 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1523>: a529.seq 1 ATGACCCATA TCAAACCCGT CATTGCCGCG CTCGCACTCA TCGGGCTTGC 51 CGCCTGCTCC GGCAGCAAAA CCGAACAGCC CAAGCTCGAC TACCAAAGCC 101 GGTCGCACCG CCTGATCAAA CTCGAAGTCC CACCTGATTT GAACAACCCC 151 GACCAAGGCA ACCTCTACCG CCTGCCTGCC GGTTCGGGCG CCGTCCGCGC 201 CAGCGATTTG GAAAAACGCC GCACACCCGC CGTCCAACAG CCTGCCGATG 251 CCGAAGTATT GAAAAGCGTC AAAGGTGTCC GCCTCGAGCG CGACGGCAGC 301 CAACGCTGGC TCGTTGTCGA CGGCAAGTCT CATGCCGAAA TCTGGCCGCT 351 CCTGAAAGCC TTTTGGCAGG AAAACGGCTT CGACATCAAA TCCGAAGAAC 401 CCGCCATCGG ACAAATGGAA ACCGAGTGGG CGGAAAACCG TGCCAAAATC 451 CCCCAAGACA GCTTGCGCCG CCTATTCGAC ACAGTCGGTT TGGGCGGCAT 501 CTACTCCACC GGCGAGCGCG ACAAATTCAT CGTCCGTATC GAACAGGGCA 551 AAAACGGCGT TTCCGACATC TTCTTCGCCC ACAAAGCCAT GAAAGAAGTG 601 TACGGCGGCA AAGACAAAGA CACGACCGTA TGGCAGCCCT CCCCGTCCGA 651 TCCCAACCTC GAAGCCGCTT TCCTGACGCG CTTTATGCAA TATTTGGGCG 701 TTGACGGACA GCAGGCGGAA AACGCATCGG CAAAAAAACC TACCCTTCCC 751 GCCGCCAACG AAATGGCGCG TATCGAAGGC AAAAGCCTGA TTGTCTTTGG 801 CGACTACGGC AGAAACTGGC GGCGCACCGC GCTCGCCCTC GACCGCATCG 851 GGCTGACCGT CGTCGGTCAA AACACCGAAC GCCACGCTTT CCTGGTTCAA 901 AAAGCCCCGA ACGAAAGCAA TGCAGTTACC GAACAAAAAC CCGGCCTGTT 951 CAAACGCCTG CTGGGCAAAG GCAAAGCGGA GAAACCTGCC GAACAGCCGG 1001 AACTGATTGT CTATGCCGAG CCTGTCGCCA ACGGCTCGCG CATCGTCCTG 1051 CTCAACAAAG ACGGCAGCGC ATATGCCGGC AAAGACGCAT CCGCATTATT 1101 GGGCAAACTC CATTCCGAAC TGCGTTAA This corresponds to the amino acid sequence <SEQ ID 1524; ORF 529. a>: a529.pep 1 MTHIKPVIAA LALIGLAACS GSKTEQPKLD YQSRSHRLIK LEVPPDLΝΝP 51 DQGΝLYRLPA GSGAVRASDL EKRRTPAVQQ PADAEVLKSV KGVRLERDGS 101 QRWLWDGKS HAEIWPLLKA FWQEΝGFDIK SEEPAIGQME TEWAEΝRAKI 151 PQDSLRRLFD TVGLGGIYST GERDKFIVRI EQGKΝGVSDI FFAHKAMKEV 201 YGGKDKDTTV WQPSPSDPΝL EAAFLTRFMQ YLGVDGQQAE ΝASAKKPTLP 251 AAΝEMARIEG KSLIVFGDYG RΝWRRTALAL DRIGLTWGQ ΝTERHAFLVQ 301 KAPΝESΝAVT EQKPGLFKRL LGKGKAEKPA EQPELIVYAE PVAΝGSRIVL 351 LΝKDGSAYAG KDASALLGKL HSELR* m529/a529 99.2% identity in 375 aa overlap 10 20 30 40 50 60 m529. pep MTHIKPVIAALALIGLAACSGSKTEQPKLDYQSRSHRLIKLEVPPDLΝΝPDQGΝLYRLPA I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a529 MTHIKPVIAALALIGLAACSGSKTEQPKLDYQSRSHRLIKLEVPPDLΝΝPDQGΝLYRLPA 10 20 30 40 50 60 70 80 90 100 110 120 m529. pep GSGAVRASDLEKRRTPAVQQPADAEVLKSVKGVRLERDGSQRWLWDGKSPAEIWPLLKA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a529 GSGAVRASDLEKRRTPAVQQPADAEVLKSVKGVRLERDGSQRWLVVDGKSHAEIWPLLKA 70 80 90 100 110 120 130 140 150 160 170 180 m529.pep FWQENGFDIKSEEPAIGQMETEWAENRAKIPQDSLRRLFDKVGLGGIYSTGERDKFIVRI I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a529 FWQENGFDIKSEEPAIGQMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRI 130 140 150 160 170 180 190 200 210 220 230 240 m529. pep EQGKNGVSDIFFAHKAMKEVYGGKDKDTTVWQPSPSDPNLEAAFLTRFMQYLGVDGQQAE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a529 EQGKNGVSDIFFAHKAMKEVYGGKDKDTTVWQPSPSDPNLEAAFLTRFMQYLGVDGQQAE 190 200 210 220 230 240 250 260 270 280 290 300 m529.pep NASAKKPTLPAANEMARIEGKSLIVFGDYGRNWRRTVLALDRIGLTVVGQNTERHAFLVQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I a529 NASAKKPTLPAANEMARIEGKSLIVFGDYGRNWRRTALALDRIGLTWGQNTERHAFLVQ 250 260 270 280 290 300 310 320 330 340 350 360 m529. pep KAPNESNAVTEQKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M I I I I a529 KAPNESNAVTEQKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAG 310 320 330 340 350 360 370 m529.pep KDASALLGKLHSELRX I I I I I I I I I I I I I I I I a529 KDASALLGKLHSELRX 370 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1525>: g530 . seq 1 atgagtgcga gcgcggcaat gacgggtttg atatgggtca tcgtgtcatc 51 ctgtgtgatg gatattaaag tgtttgtcat gttatgccgt ccgaacggtt 101 cagacggcat ggctatattt aaagttgtcc tgaggctttc agggcggcgc 151 ggacttttgc ctgtccgcct tccgtcagcg gaacgagcgg caggcgcacg 201 tgcggtccgc atccgcccaa ggcggatacc gcccatttcg gtgcggcggg 251 actgggttcg cagaacatgg tgtcgtaaat cggaatcagc cggtcgttga This corresponds to the amino acid sequence <SEQ ID 1526; ORF 530.ng>: g530.pep 1 MSASAAMTGL IWVIVSSCVM DIKVFVMLCR PΝGSDGMAIF KWLRLSGRR 51 GLLPVRLPSA ERAAGARAVR IRPRRIPPIS VRRDWVRRTW CRKSESAGR* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1527>: m530. seq 1 wTGAGTGCGA GCGCGGCAAT GACGGGTyTG ATATGGGTCA TCGTGTCATC 51 sTGTGTGATG GATATTAAAG TGTyTGTTGC GwTATGCCGT CCGAACGGTT 101 CGGACGGCAT GGmTATATTT AAAGTTGTCC TGAGGCTTTC AGGGCGGCGC 151 GGACTkTTGC TGTCCGTTT yCCGTCAGCG GAACGAGCGG CAGGCGGACG 201 TGCGGTTCGC ATCTGCCCAg GGCGGATACC GCCCATTTCG GTGCGGCGGG 251 GCTGGGTTCG CAGAACATGG TGTCGTAAAT CGGAATCAGT CGGTCGTTGA This corresponds to the amino acid sequence <SEQ ID 1528; ORF 530>: m530.pep 1 XSASAAMTGL IWVIVSSCVM DIKVXVAXCR PΝGSDGMXIF KWLRLSGRR 51 GLLXVRFPSA ERAAGGRAVR ICPGRIPPIS VRRGWVRRTW CRKSESVGR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 530 shows 88.8% identity over a 98 aa overlap with a predicted ORF (ORF 530.ng) from N gonorrhoeae: m530/g530 m530.pep XSASAAMTGLIWVIVSSCVMDIKVXVAXCRPΝGSDGMXIFKWLRLSGRRGLLXVRFPSA 60 I II I I I I I I I I I I I I I I I I I I I I I lllllllll lllllllllllllll I I : I I I g530 MSASAAMTGLIWVIVSSCVMDIKVFVMLCRPNGSDGMAIFKWLRLSGRRGLLPVRLPSA 60 10 20 30 40 50 60 m530.pep ERAAGGRAVRICPGRIPPISVRRGWVRRTWCRKSESVGR 99 I I I I I : I I I I I I lllllllll I I I I I I I I I I I I : I I g530 ERAAGARAVRIRPRRIPPISVRRDWVRRTWCRKSESAGR 99 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1529>: a530. seq 1 ATGAGTGCGA GCGCGGCAAT GACGGGTTTG ATATGGGTCA TCGTGTCATC 51 CTGTGTGATG GATATTAAAG TGTTTGTTGC GTTATGCCGT CCGAACGGTT 101 CGGACGGCAT GGCTATATTT AAAGTTGTCC TGAGGCTTTC AGGGCGGCGC 151 GGACTTTTGC CTGTCCGCCT TCCGTCAGCG GAACGAGCGG CAGGCGGACG 201 TGCGGTTCGC ATCTGCCCAG GGCGGATACC GCCCATTTCG GTGCGGCGGG 251 GCTGGGTTCG CAGAACATGG TGTCGTAAAT CGGAATCAGC CGGTCGTTGA This corresponds to the amino acid sequence <SEQ ID 1530; ORF 530. a>: a530.pep 1 MSASAAMTGL IWVIVSSCVM DIKVFVALCR PΝGSDGMAIF KWLRLSGRR 51 GLLPVRLPSA ERAAGGRAVR ICPGRIPPIS VRRGWVRRTW CRKSESAGR* m530/a530 93.9% identity in 98 aa overlap 10 20 30 40 50 60 m530. pep XSASAAMTGLIWVIVSSCVMDIKVXVAXCRPΝGSDGMXIFKWLRLSGRRGLLXVRFPSA I I I I I I I I I I I I I I I I I I I II I I II lllllllll lllllllllllllll I I: I I I a530 MSASAAMTGLIWVIVSSCVMDIKVFVALCRPΝGSDGMAIFKWLRLSGRRGLLPVRLPSA 10 20 30 40 50 60 70 80 90 100 m530.pep ERAAGGRAVRICPGRIPPISVRRGWVRRTWCRKSESVGRX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I: I I I a530 ERAAGGRAVRICPGRIPPISVRRGWVRRTWCRKSESAGRX 70 80 90 100 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 1531>: g531.seq 1 ATGACCGCCC TACTCGTCAT CCTCGCCCTC GCCCTGATAG CCGTCGGCAC 51 GGCAGGCATC GTCTATCCCG CCCTGCCCGG CTTGGCATTG ATGTTTGCCG 101 GAACATGGCT GCTTGCCTAT GCCGGCGGCT ATCAAATCTA CGGCGCAGGC 151 ATCTTGTGGA CGGTCGGACT CATCAGCCTT GGCGGCATAC TGGCGGACTA 201 TATGGCAGGC ATGTTGGGGG TAAAATACAC TGGGGCAGGC AAACTCGCCG 251 TCCGAGGTGC ATTGGCCGGC AGCATCATCG GCATATTTTT CTCCCTTCCC 301 GGACTAATAC TCGGCCCCTT TATCGGCGCG GCGGCAGGCG AACTGATCGA 351 TCGGCGCAAT ATGCTTCAGG CAGGTAAAGC GGGCTTGGGT ACGCTGTTGG 401 GGCTTGTCGT CGGCACGGCG TTCAAAATCG GCTGCGCCGT ATCCATCTTG 451 TTTATCCTGT TGGTGAAATA CATCGCATAC CTGTTTTAA This corresponds to the amino acid sequence <SEQ ID 1532; ORF 531.ng>: g531.pep 1 MTALLVILAL ALIAVGTAGI VYPALPGLAL MFAGTWLLAY AGGYQIYGAG 51 ILWTVGLISL GGILADYMAG MLGVKYTGAG KLAVRGALAG SIIGIFFSLP 101 GLILGPFIGA AAGELIDRRΝ MLQAGKAGLG TLLGLWGTA FKIGCAVSIL 151 FILLVKYIAY LF The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 1533>: m531. seq 1 ATGACCGTAC TGACCGTCAT CCTCGCCCTC GCCCTGATAG CCGTCGGCAC 51 GGCGGGCATC GTTTaCCCCG CCCTGCCCGG ATTGGCATTG ATGTTTGCCG 101 GAACATGGCT GCTTGCCTAT GCCGGCGGCT ACCAAATCTA CGGCGCGGGC 151 GTTTTGTGGA CGGTCGGACT CATCAGCCTT GCCGGCATAC TGGCGGACTA 201 TGTGGCAGGC ATATGGGGGA CAAAATATAC CGGAGCGGGC AAGCTCGCCG 251 TTCGCGGCGC ATTGGCCGGC AGCATCATCG GCATATTTTT CTCCCTTCCC 301 GGACTAATAC TCGGTCCCTT TATCGGCGCG GCGGCAGGCG AACTGATCGA 351 ACGGCGCAAT ATGCTTCAGG CAGGTAAAGC GGGCTTGGGT ACGCTGTTGG 401 GGCTTGTCGT CGGCACGGCG TTCAAAATCG GCTGCGCnGT ATCCATCTTG 451 TTTATCCTGT TGGTGAaATA CATCGCCTAC CTGTTTTAA This corresponds to the amino acid sequence <SEQ ID 1534; ORF 531>: m531.pep 1 MTVLTVILAL ALIAVGTAGI VYPALPGLAL MFAGTWLLAY AGGYQIYGAG 51 VLWTVGLISL AGILADYVAG IWGTKYTGAG KLAVRGALAG SIIGIFFSLP 101 GLILGPFIGA AAGELIERRN MLQAGKAGLG TLLGLWGTA FKIGCAVSIL 151 FILLVKYIAY LF* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 531 shows 94.4% identity over a 162 aa overlap with a predicted ORF (ORF 53 l.ng) from N gonorrhoeae: m53l/g531 10 20 30 40 50 60 m531.pep MTVLTVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYAGGYQIYGAGVLWTVGLISL MM MMMMMMMMMMMMMMMMMMIMMMMMMMMM g531 MTALLVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYAGGYQIYGAGILWTVGLISL 10 20 30 40 50 60 70 80 90 100 110 120 m531.pep AGILADYVAGIWGTKYTGAGKLAVRGALAGSIIGIFFSLPGLILGPFIGAAAGELIERRΝ M I I I M h I M M M M M M M M M M M M M M M M M M I M M M M M g531 GGILADYMAGMLGVKYTGAGKLAVRGALAGSIIGIFFSLPGLILGPFIGAAAGELIDRRΝ 70 80 90 100 110 120 130 140 150 160 m531 . pep MLQAGKAGLGTLLGLWGTAFKIGCAVSILFILLVKYIAYLF MM MMIMI I M Ml I M MM : M g531 MLQAGKAGLGTLLGLWGTAFKIGCAVSILFILLVKYIAYLF 130 140 150 160 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1535>: a531. seq 1 ATGACCGCCT TGCTCGTCAT CCTCGCCCTC GCCCTGATAG CCGCCGGTAC 51 GGCGGGCATC GTTTACCCCG CCCTGCCCGG ATTGGCATTG ATGTTTGCCG 101 GAACCTGGCT GCTCGCCTAC TCCGGCGGCT ACCAAATCTA CGGCGCGGGC 151 GTTTTGTGGA CGGTCGGACT CATCAGCCTT GCCGGCATAC TGGCGGACTA 201 TGTGGCAGGC ATATGGGGGA CAAAATATAC CGGAGCGGGC AAGCTCGCCG 251 TTCGCGGCGC ATTGGCCGGC AGCATCATCG GCATATTTTT CTCCCTTCCC 301 GGACTAATAC TCGGTCCCTT TATCGGCGCG GCGGCAGGCG AACTGATCGA 351 ACGGCGCAAT ATGCTTCAGG CAGGTAAAGC GGGCTTGGGT ACGCTGTTGG 401 GGCTTATCGT CGGTACGGCG TTCAAAATCG GCTGCGCCGT ATCCATCTTG 451 TTTATCCTGT TGGTGAAATA CATCGCCTAC CTGTTTTAA This corresponds to the amino acid sequence <SEQ ID 1536; ORF 531.a>: a531.pep 1 MTALLVILAL ALIAAGTAGI VYPALPGLAL MFAGTWLLAY SGGYQIYGAG 51 VLWTVGLISL AGILADYVAG IWGTKYTGAG KLAVRGALAG SIIGIFFSLP 101 GLILGPFIGA AAGELIERRN MLQAGKAGLG TLLGLIVGTA FKIGCAVSIL 151 FILLVKYIAY LF* m53 l/a531 96.9% identity in 162 aa overlap 10 20 30 40 50 60 m531. pep MTVLTVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYAGGYQIYGAGVLWTVGLISL 11:1 I I M I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I II I I I I I I I I I l a531 MTALLVILALALIAAGTAGIVYPALPGLALMFAGTWLLAYSGGYQIYGAGVLWTVGLIΞL 10 20 30 40 50 60 70 80 90 100 110 120 m531.pep AGILADYVAGIWGTKYTGAGKLAVRGALAGSIIGIFFSLPGLILGPFIGAAAGELIERRN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a531 AGILADYVAGIWGTKYTGAGKLAVRGALAGSIIGIFFSLPGLILGPFIGAAAGELIERRN 70 80 90 100 110 120 130 140 150 160 m531.pep MLQAGKAGLGTLLGLWGTAFKIGCAVSILFILLVKYIAYLFX I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I a531 MLQAGKAGLGTLLGLIVGTAFKIGCAVSILFILLVKYIAYLFX 130 140 150 160 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1537>: g532.seq (partial) 1 atggctgaaa caatgaaaaa acaggcggat tcgcctgatt tggtgtacgg 51 tttggaagac aggccgccgt tcggtaatgc gctcttgagc gcggttaccc 101 atcttttggc gattttcgtg ccgatgatta cgcccgcgct gattgtgggc 151 ggcgcgctgg aattgccggt ggagatgacg gcgtatctgg tgtcgatggc 201 gatggttgcg tcgggtgtcg gcacttattt gcaggtcaac cgcttcgggt 251 cggtcggctc ggggatgctg tccatccagc gttaccgtca tgattgcgct 301 cggcgcgggg atgaaagagg gcggtttgag ... This corresponds to the amino acid sequence <SEQ ID 1538; ORF 532.ng>: g532.pep (partial) 1 MAETMKKQAD SPDLVYGLED RPPFGΝALLS AVTHLLAIFV PMITPALIVG 51 GALELPVEMT AYLVSMAMVA SGVGTYLQVΝ RFGSVGSGML SIQRYRHDCA 101 RRGDERGRFE ... The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1539>: m532. seq 1 ATGAGCGGTC AGTTGGGCAA AGGTGCGGAT GCGCCTGATT TGGTGTACGG 51 TTTGGAAGAC AGGCCGCCGT TCGGTAATGC GCTCTTGAGC GCGGTTACCC 101 ATCTTTTGGC GATTTTTGTG CCGATGATTA CGCCCGCGCT GATTGTGGGC 151 GGCGCGCTGG AATTGCCGGT GGAGATGACG GCGTATCTCG TGTCGATGGC 201 GATGGTTGCG TCGGGTGTCG GCACTTATTT GCAGGTCAAC CGCTTCGGGC 251 CGGTCGGTTC GGGGATGCTG TCCATCCAGT CGGTGAATTT TTCGTTCGTT 301 ACCGTGATGA TTGCGCTGGG CGCGGGGATG AAAGAGGGCG GTTTGACTAA 351 GGATGCGATG ATTTCGACGC TCTTGGGCGT ATCGTTTGTC GGCGCGTTTT 401 TGGTGTGTTT CTCGGCGTGG CTTCTGCCGT ATTTGAAAAA AGTGATTACG 451 CCGACGGTCA GCGGCGTGGT CGTGATGCTC ATTGGTTTGA GTTTGGTACA 501 CGTCGGCATT ACCGATTTCG GCGGCGGCTT CGGCGCGAAG GCGGACGGCA 551 CGTTCGGCTC GATGGAAAAC TTGGGGCTGG CATCGCTGGT GTTGCTGATT 601 GTGTTGGTGT TCAACTGCAT GAAAAACCCG CTGTTGCGCA TGAGCGGCAT 651 TGCGGTCGGG CTGATTGCCG GCTATATCGT CGCGCTGTTT TTGGGCAAGG 701 TGGATTTTTC CGCGCTGCAA AACCTGCCGC TGGTTACGCT GCCCGTACCG 751 TTTAAATACG GTTTTGCTTT CGACTGGCAC GCGTTTATTG TGGCGGGCGC 801 GATTTTCTTG TTGAGCGTGT TTGAGGCGGT CGGCGATTTA ACCGCGACGG 851 CAATGGTGTC CGACCAGCCG ATTGAAGGCG AGGAATACAC CAAACGCCTG 901 CGCGGCGGCG TGTTGGCTGA CGGCTTGGTG TCGGTGATTG CGACGGCTTT 951 GGGTTCGCTG CCGCTGACGA CGTTTGCGCA AAACAACGGC GTGATTCAGA 1001 TGACCGGCGT GGCTTCGCGC CATGTGGGCA AATATATTGC CGTGATTTTG 1051 GTGCTGTTGG GTCTGTTCCC CGTTGTCGGT CGCGCGTTTA CGACGATTCC 1101 GAGTCCGGTG TTGGGCGGCG CGATGGTTTT GATGTTCGGC TTAATTGCGA 1151 TTGCGGGCGT GCGGATTTTG GTCAGTCACG GCATCCGCAG GCGCGAAGCG 1201 GTGATTGCGG CAACGTCGGT CGGTTTGGGC TTGGGTGTCG CGTTTGAGCC 1251 GGAAGTGTTT AAAAACCTGC CCGTCTTGTT CCAAAACTCT ATTTCCGCCG 1301 GCGGCATTAC GGCAGTCTTG CTGAATTTGG TCTTGCCCGA AGATAAAACC 1351 GAGGCGGCGG TCAAGTTTGA TACCGACCAC TTGGAACACT GA This corresponds to the amino acid sequence <SEQ ID 1540; ORF 532>: m532.pep 1 MSGQLGKGAD APDLVYGLED RPPFGΝALLS AVTHLLAIFV PMITPALIVG 51 GALELPVEMT AYLVSMAMVA SGVGTYLQVΝ RFGPVGSGML SIQSVΝFSFV 101 TVMIALGAGM KEGGLTKDAM ISTLLGVSFV GAFLVCFSAW LLPYLKKVIT 151 PTVSGVWML IGLSLVHVGI TDFGGGFGAK ADGTFGSMEΝ LGLASLVLLI 201 VLVFNCMKNP LLRMSGIAVG LIAGYIVALF LGKVDFSALQ NLPLVTLPVP 251 FKYGFAFDWH AFIVAGAIFL LSVFEAVGDL TATAMVSDQP IEGEEYTKRL 301 RGGVLADGLV SVIATALGSL PLTTFAQNNG VIQMTGVASR HVGKYIAVIL 351 VLLGLFPWG RAFTTIPSPV LGGAMVLMFG LIAIAGVRIL VSHGIRRREA 401 VIAATSVGLG LGVAFEPEVF KNLPVLFQNS ISAGGITAVL LNLVLPEDKT 451 EAAVKFDTDH LEH* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF532 shows 91.4% identity over a 93 aa overlap with a predicted ORF (ORF 532.ng) from N gonorrhoeae: g532/m532 10 20 30 40 50 60 g532.pep MAETMKKQADSPDLVYGLEDRPPFGΝALLSAVTHLLAIFVPMITPALIVGGALELPVEMT h = I MMMMMMMMMMMMMMMMMMMMMMMMMM m532 MSGQLGKGADAPDLVYGLEDRPPFGΝALLSAVTHLLAIFVPMITPALIVGGALELPVEMT 10 20 30 40 50 60 70 80 90 100 110 g532. pep AYLVSMAMVASGVGTYLQVΝRFGSVGSGMLSIQRYRHDCARRGDERGRFEX ! j I M 11111111111 j 11111 ! I i 11 E I E 11 m532 AYLVSMAMVASGVGTYLQVΝRFGPVGSGMLSIQSVΝFSFVTVMIALGAGMKEGGLTKDAM 70 80 90 100 110 120 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1541>: a532. seq 1 ATGAGCGGTC AGTTGGGCAA AGGTGCGGAT GCGCCTGATT TGGTGTACGG 51 TTTGGAGGAT AGGCCGCCGT TCGGTAATGC GCTCTTGAGC GCGGTTACCC 101 ATCTTTTGGC GATTTTTGTG CCGATGATTA CGCCCGCGCT GATTGTGGGC 151 GGCGCGCTGG AATTGCCGGT GGAGATGACG GCGTATCTCG TGTCGATGGC 201 GATGGTTGCG TCGGGTGTCG GCACTTATTT GCAGGTCAAC CGCTTCGGGC 251 CGGTCGGTTC GGGGATGCTG TCCATCCAGT CGGTGAATTT CTCGTTCGTT 301 ACCGTCATGA TTGCGCTCGG CGCGGGGATG AAAGAGGGCG GTTTGACTAA 351 GGATGCGATG ATTTCGACGC TCTTGGGCGT ATCGTTTGTC GGCGCGTTTT 401 TGGTGTGTTT TTCGGCGTGG CTTCTGCCGT ATTTGAAAAA AGTGATTACG 451 CCGACGGTCA GCGGTGTGGT GGTGATGCTG ATCGGCTTGA GTTTGGTACA 501 CGTCGGTATT ACCGATTTCG GCGGCGGCTT CGGCGCAAAG GCGGACGGCA 551 CGTTCGGCTC GATGGAAAAC TTGGGGCTGG CATCGCTGGT GCTGCTGATT 601 GTGCTGGTGT TCAATTGCAT GAAAAACCCG CTGCTGCGGA TGAGCGGCAT 651 TGCGGTCGGT CTGATTGCCG GCTATATCGT CGCGCTGTTT TTGGGCAAGG 701 TGGATTTTTC GGCACTGCAA AACCTGCCGC TGGTTACGCT GCCCGTACCG 751 TTTAAATATG GTTTTGCTTT TGACTGGCAC GCATTTATTG TGGCGGGTGC 801 GATTTTCTTG TTGAGCGTGT TTGAGGCGGT CGGCGATTTG ACGGCGACGG 851 CAATGGTGTC CGACCAGCCG ATTGAAGGCG AGGAATACAC CAAACGCTTG 901 CGCGGCGGCG TGTTGGCGGA CGGCTTGGTG TCGGTGATTG CGACGGCTTT 951 GGGTTCGCTG CCGCTGACGA CGTTTGCACA AAACAACGGC GTGATTCAGA 1001 TGACCGGCGT GGCTTCGCGC CATGTGGGCA AATATATTGC CGTGATTTTG 1051 GTGCTGTTGG GTCTGTTCCC CGTTGTCGGA CGCGCGTTTA CGACGATTCC 1101 GAGTCCGGTG TTGGGCGGCG CGATGGTTTT GATGTTCGGC TTGATTGCGA 1151 TTGCGGGCGT GCGGATTTTG GTCAGCCACG GCATCCGCAG GCGCGAAGCG 1201 GTAATTGCGG CAACGTCGGT CGGTTTGGGC TTGGGTGTCG CGTTTGAGCC 1251 GGAAGTGTTT AAAAACCTGC CCGTCTTGTT CCAAAACTCT ATTTCCGCCG 1301 GCGGCATTAC GGCAGTCTTG CTGAATTTGG TCTTGCCCGA AGATAAAACC 1351 GAGGCGGCGG TCAAGTTTGA TACCGACCAC TTGGAACACT GA This corresponds to the amino acid sequence <SEQ ID 1542; ORF 532. a>: a532.pep 1 MSGQLGKGAD APDLVYGLED RPPFGΝALLS AVTHLLAIFV PMITPALIVG 51 GALELPVEMT AYLVSMAMVA SGVGTYLQVΝ RFGPVGSGML SIQSVΝFSFV 101 TVMIALGAGM KEGGLTKDAM ISTLLGVSFV GAFLVCFSAW LLPYLKKVIT 151 PTVSGVVVML IGLSLVHVGI TDFGGGFGAK ADGTFGSMEN LGLASLVLLI 201 VLVFNCMKNP LLRMSGIAVG LIAGYIVALF LGKVDFSALQ NLPLVTLPVP 251 FKYGFAFDWH AFIVAGAIFL LSVFEAVGDL TATAMVSDQP lEGEEYTKRL 301 RGGVLADGLV SVIATALGSL PLTTFAQNNG VIQMTGVASR HVGKYIAVIL 351 VLLGLFPVVG RAFTTIPSPV LGGAMVLMFG LIAIAGVRIL VSHGIRRREA 401 VIAATSVGLG LGVAFEPEVF KNLPVLFQNS ISAGGITAVL LNLVLPEDKT 451 EAAVKFDTDH LEH* m532/a532 100.0% identity in 463 aa overlap 10 20 30 40 50 60 m532.pep MSGQLGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMITPALIVGGALELPVEMT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a532 MSGQLGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMITPALIVGGALELPVEMT 10 20 30 40 50 60 70 80 90 100 110 120 m532 .pep AYLVSMAMVASGVGTYLQVNRFGPVGSGMLΞIQSVNFSFVTVMIALGAGMKEGGLTKDAM I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a532 AYLVSMA VASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTVMIALGAGMKEGGLTKDAM 70 80 90 100 110 120 130 140 150 160 170 180 m532 .pep ISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVMLIGLSLVHVGITDFGGGFGAK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I a532 ISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVV LIGLSLVHVGITDFGGGFGAK 130 140 150 160 170 180 190 200 210 220 230 240 m532 .pep ADGTFGSMENLGLASLVLLIVLVFNCMKNPLLRMSGIAVGLIAGYIVALFLGKVDFSALQ I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I a532 ADGTFGSMENLGLAΞLVLLIVLVFNC KNPLLR SGIAVGLIAGYIVALFLGKVDFSALQ 190 200 210 220 230 240 250 260 270 280 290 300 m532, .pep NLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLΞVFEAVGDLTATAMVSDQPIEGEEYTKRL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a532 NLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEAVGDLTATAMVSDQPIEGEEYTKRL 250 260 270 280 290 300 310 320 330 340 350 360 m532, .pep RGGVLADGLVΞVIATALGΞLPLTTFAQNNGVIQMTGVASRHVGKYIAVILVLLGLFPVVG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a532 RGGVLADGLVSVIATALGSLPLTTFAQNNGVIQMTGVASRHVGKYIAVILVLLGLFPVVG 310 320 330 340 350 360 370 380 390 400 410 420 m532. •pep RAFTTIPSPVLGGAMVLMFGLIAIAGVRILVSHGIRRREAVIAATSVGLGLGVAFEPEVF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a532 RAFTTIPΞPVLGGAMVLMFGLIAIAGVRILVSHGIRRREAVIAATSVGLGLGVAFEPEVF 370 380 390 400 410 420 430 440 450 460 m532. •pep KNLPVLFQNSISAGGITAVLLNLVLPEDKTEAAVKFDTDHLEHX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I II I I I I a532 KNLPVLFQNSISAGGITAVLLNLVLPEDKTEAAVKFDTDHLEHX 430 440 450 460 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1543>: g535. seq 1 atgccctttc ccgttttcag acaantattt gcttngtcct tgctacggtt 51 ttttgccgta ggtcggattc tcgaatccga catttccaac agcggttttt 101 cggaaacgat aaacgcgtca aatgtttttt ttgtcggata cgaatatccg 151 gcctgcattt caaatttaca tcgcttccaa tttcgcaaac ttggtatcca 201 gttctttcac gccctgtttg ccgaagttga tggtcagtcg ggcggattcg 251 cctttgtctg cggcatcgat aatcacgccg gtgccgaatt tggcgtgacg 301 gacgttttgt ccgatgcgga agcctgcgta ggtttgcggc tgtttgaagt 351 catcgatgat tttgtcccgt tgtacggtgg tttggcgcgt gttgccgtag 401 ctgtcgaagg cgggtttttt gacggacagg tagtgcaata cttctggcgg 451 gatttcttcg acgaagcggg atgcgatgcc gaattgggtt tgtccgtgca 501 gcatgcgttg ctgtgccatg gtgatgtaga ggcgtttgcg ggcgcgggtg 551 atggcgacgt acatgaggcg gcgttcttct tcgaggccgc cgcgctcggc 601 aaggctcatt tcgctgggga aacgcccctc ttccataccg gtgaggaaga 651 cggcgttgaa ttccaagcct ttggcggcgt ggacggtcat cagttggacg 701 gctttttcgc ctgcccctgc ttggttttcg ccggattcga gggcggcgtt 751 gctcaagaag gcgaggatgg ggaaggcggg atcgtctga This corresponds to the amino acid sequence <SEQ ID 1544; ORF 535.ng>: g535.pep 1 MPFPVFRQXF AXSLLRFFAV GRILESDISN SGFSETINAS NVFFVGYEYP 51 ACISNLHRFQ FRKLGIQFFH ALFAEVDGQS GGFAFVCGID NHAGAEFGVT 101 DVLSDAEACV GLRLFEVIDD FVPLYGGLAR VAVAVEGGFF DGQWQYFWR 151 DFFDEAGCDA ELGLSVQHAL LCHGDVEAFA GAGDGDVHEA AFFFEAAALG 201 KAHFAGETPL FHTGEEDGVE FQAFGGVDGH QLDGFFACPC LVFAGFEGGV 251 AQEGEDGEGG IV* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1545>: m535. seq 1 aTGCCCTTtC CCGTTTTCAG ACGGCCTTTT GCTTTGTCCT TACTtACGTT 51 TTTTGCCGTA AGTCAGATTC TTGTATCCGA CATTTCCAAC AGCGGTGTTT 101 CGGAAACAAT AGACGCGTCA AATGTTTTTG TCGGATACGA ATATCCGACC 151 TACATTTCAA ATTTACATCT CTTCCAATTT CGCAAACTTG GTGTCCAACT 201 CTTTCACGCC CTGTTTGCCG AAATTGATGG TCAGTCGGGC GGATTCGCCT 251 TTATCTGCGG CATCGATAAT CACGCCGGTG CCGAATTTGG CGTGGCGGAC 301 GTTTTGTCCG ATACGGAAAC CTGCGTAGGT TTGGGGCTGT TTGTAGTCGT 351 CGATGATTTT ATCTTTGGAT GCGGCGGTTT GGCGCGTGTT GCCGTAACTG 401 TCGTAGGCAG GCTTTTTGAC GGACAGGTAG TGCAATACTT CGGGTGGGAT 451 CTCTTCGACG AAGCGGGAGA CGATGCCGAA TTGGGTTTGT CCGTGCAGCA 501 TGCGTTGTTG CGCCATGGTG ATGTAGAGGC GTTTGCGGGC GCGGGTGATG 551 GCGACGTACA TGAGGCGGCG TTCTTCTTCG AGGCCGCCGC GTTCGGCAAG 601 GCTCATTTCG CTGGGGAAGC GGCCTTCTTC CATGCCGGTG AGGAAGACGG 651 CGTTAAATTC CAAGCCTTTG GCGGCGTGGA CGGTCATGAG TTGGACGGCC 701 TTTTCGCCTG CGCCTGCCTG GTTTTCACCG GATTCGAGGG CGGCATTGCT 751 TAGGAAGGCG AGAATGGGGA AGGCGGGGTC GTCTGA This corresponds to the amino acid sequence <SEQ ID 1546; ORF 535>: m535.pep 1 MPFPVFRRPF ALSLLTFFAV SQILVSDISΝ SGVSETIDAS ΝVFVGYEYPT 51 YISΝLHLFQF RKLGVQLFHA LFAEIDGQSG GFAFICGIDΝ HAGAEFGVAD 101 VLSDTETCVG LGLFVWDDF IFGCGGLARV AVT GRLFD GQWQYFGWD 151 LFDEAGDDAE LGLSVQHALL RHGDVEAFAG AGDGDVHEAA FFFEAAAFGK 201 AHFAGEAAFF HAGEEDGVKF QAFGGVDGHE LDGLFACACL VFTGFEGGIA 251 XEGEΝGEGGV V* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N. gonorrhoeae ORF 535 shows 80.9% identity over a 262 aa overlap with a predicted ORF (ORF 535.ng) from N gonorrhoeae: m535/g535 10 20 30 40 50 59 m535.pep MPFPVFRRPFALSLLTFFAVSQILVSDISΝSGVSETIDASΝVF-VGYEYPTYISΝLHLFQ M I I I I M I I I I I M l h M I l l l l l l l I M l l l l l l l : M i l l I I g535 MPFPVFRQXFAXSLLRFFAVGRILESDISΝSGFSETIΝASΝVFFVGYEYPACISΝLHRFQ 10 20 30 40 50 60 60 70 80 90 100 110 119 m535.pep FRKLGVQLFHALFAEIDG Q SGGFAFICGIDΝHAGAEFGVADVLSDTETCVGLGLFVWDD M M I M M M M M M M M M M M M M M M M M M M M M M I M I I I M I g535 FRKLGIQFFHALFAEVDGQSGGFAFVCGIDΝHAGAEFGVTDVLSDAEACVGLRLFEVIDD 70 80 90 100 110 120 120 130 140 150 160 170 179 m535. pep FIFGCGGLARVAVTWGRLFDGQWQYFGWDLFDEAGDDAELGLSVQHALLRHGDVEAFA h IIIIMMM I MIIIIIM! IMMM MMMIMMM MINIM g535 FVPLYGGLARVAVAVEGGFFDGQWQYFWRDFFDEAGCDAELGLSVQHALLCHGDVEAFA 130 140 150 160 170 180 180 190 200 210 220 230 239 m535. pep GAGDGDVHEAAFFFEAAAFGKAHFAGEAAFFHAGEEDGVKFQAFGGVDGHELDGLFACAC MMMMMMMMMMIMMIh M I M II II I M II II I II 11 M II M 11 I g535 GAGDGDVHEAAFFFEAAALGKAHFAGETPLFHTGEEDGVEFQAFGGVDGHQLDGFFACPC 190 200 210 220 230 240 240 250 260 m535. pep LVFTGFEGGIAXEGENGEGGW IMMMIIM IIIMIMM g535 LVFAGFEGGVAQEGEDGEGGIV 250 260 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1547>: a535.seq (partial) 1 TTCAGACGGC CTTTTGCCTT GTCCTTGCTA CAGTTTTTTG CCATAGGTCG 51 GATTCTCGAA TCCGACATTT CCAACAGCGG TTTTTCGGAA ACGATAGACG 101 CGTCAAATAT TTTTGTCGGA TACGAGTATC CAGCCTGCAT TTCAAATTTA 151 CATCGCTTCC AATTTCGCAA ACTTGGTGTC CAACTCTTTC ACGCCCTGTT 201 TGCCGAAATT GATGGTCAGT CGGGCGGATT CGCCTTTATC TGCGGCATCG 251 ATAATCACGC CGGTGCCGAA TTTGGCGTGG CGGACGTTTT GTCCGATACG 301 GAAACCTGCG TAGGTTTGGG GCTGTTTGTA GTCGTCGATG ATTTTGTCTT 351 TGGGCGCGGC GGTTTGGCGC GTGTTGCCAT AGCGGTCGTA GGCGGGTTTT 401 TTGACGGACA GGTAGTGCAA TACTTCGGGC GGGATTTCTT CGACGAAGCG 451 GGAGACGATG CCGAATTGGG TTTGTCCGTG CAGCATGCGT TGTTGCGCCA 501 TGGTGATGTA GAGGCGTTTG CGGGCGCGGG TGATGGCGAC GTACATCAGG 551 CGGCGTTCTT CTTCGAGGCC GCCGCGTTCG GCAAGGCTCA TTTCGCTGGG 601 GAAGCGGCCT TCTTCCATGC CGGTGAGGAA TACGGCGTTA AATTCCAAGC 651 CTTTGGCGGC GTGCACGGTC ATGAGTTGTA CGGCTTTTTC GCCCGCGCCT 701 GCTTGGTTTT CGCCGGATTC GAGAGCAGCA TTGCTTAGGA AAGCGAGGAT 751 GGGGAAGGCG GGGTCGTCTG A This corresponds to the amino acid sequence <SEQ ID 1548; ORF 535. a>: a535.pep (partial) 1 FRRPFALSLL QFFAIGRILE SDISΝSGFSE TIDASΝIFVG YEYPACISΝL 51 HRFQFRKLGV QLFHALFAEI DGQSGGFAFI CGIDΝHAGAE FGVADVLSDT 101 ETCVGLGLFV WDDFVFGRG GLARVAIAVV GGFFDGQWQ YFGRDFFDEA 151 GDDAELGLSV QHALLRHGDV EAFAGAGDGD VHQAAFFFEA AAFGKAHFAG 201 EAAFFHAGEE YGVKFQAFGG VHGHELYGFF ARACLVFAGF ESSIA*ESED 251 GEGGVV* m535/a535 88.7% identity in 256 aa overlap 10 20 30 40 50 60 m535.pep MPFPVFRRPFALSLLTFFAVSQILVSDISΝSGVSETIDASΝVFVGYEYPTYISΝLHLFQF I I I I II I II I I I I::: I I MIIIM II II I II I : II II II I : II II I III a535 FRRPFALSLLQFFAIGRILESDISΝSGFSETIDASΝIFVGYEYPACISΝLHRFQF 10 20 30 40 50 70 80 90 100 110 120 m535. pep RKLGVQLFHALFAEIDGQSGGFAFICGIDΝHAGAEFGVADVLSDTETCVGLGLFVWDDF II II I II II I I II II I I I I I II II I I I I I II II I I I II II II I II II II I II II I ! I II I a535 RKLGVQLFHALFAEIDGQSGGFAFICGIDΝHAGAEFGVADVLSDTETCVGLGLFVWDDF 60 70 80 90 100 110 130 140 150 160 170 180 m535. pep IFGCGGLARVAVTWGRLFDGQWQYFGWDLFDEAGDDAELGLSVQHALLRHGDVEAFAG l I II II II :M II M I II II I I II I M I I II II II II II II II II II I I I I II II a535 VFGRGGLARVAIAWGGFFDGQVVQYFGRDFFDEAGDDAELGLSVQHALLRHGDVEAFAG 120 130 140 150 160 170 190 200 210 220 230 240 m535. pep AGDGDVHEAAFFFEAAAFGKAHFAGEAAFFHAGEEDGVKFQAFGGVDGHELDGLFACACL I I I I II |: I I II II II I I II I II I I I I II I II II I II I I I I II II I II I I M I III a535 AGDGDVHQAAFFFEAAAFGKAHFAGEAAFFHAGEEYGVKFQAFGGVHGHELYGFFARACL 180 190 200 210 220 230 250 260 m535.pep VFTGFEGGIAXEGENGEGGVVX II M II :M I II MM I I II II a535 VFAGFESSIAXESEDGEGGWX 240 250 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1549>: g537.seq 1 atgaaatccc tttttatttg gctgcttcta ttgggctcgg cggcaggcgt 51 tttctaccat acccaaaacc aatccctgcc cgcgggcgaa cttgtctatc 101 cgtccgcacc gcaaatcagg gacggcggcg atgcgctgca ctacctcaac 151 cgcatccgca cacaaatcgg tttgcacgcg ctggcacacg cgccggtttt 201 ggaaaattcc gcccgcaggc acgcacgcta tctcacgctc aatcccgaag 251 acggacacgg cgaacaccat cccgacaatc cgcactacac cgcacaaaag 301 ctgaccgaac gcacacgcct tgccgggtat ctctacaacg gcgtgcatga 351 aaacatcagc acggaagagg aagccgccga atcgtccgac agcgacatcc 401 gcacgcagca acgccaagtg gacgctttga tgagcgcaat ctaccaccgc 451 ctttcgctgc ttgaccgcca taccgacgaa gcaggtgcgg catttgtgcg 501 cgaaaacggc aaaaccgtcc tcgtattcaa tcagggcaac ggcagcttcg 551 agcgcgcctg tgcaaaagga aggcggcagc cggaagcagg acggaaatat 601 taccgcaacg cttgccacaa cggtgcggcc gtttatgctg acgaagccat 651 gcccgtaacg gaattgcttt ataccgccta tccggttggc ggcggcgcgc 701 tgccttattt ttacggggaa cgtcccgacc ccgtgccgga atatgaaatc 751 acaggcaatc ctgccagcat tgatttttcc gaggcggcag gcaaaattgc 801 gatgaaaagt ttcaagctgt atcagggtaa aaacgaaatc cgccccgtca 851 gggttttaac cgccggcaac gaccctaacg gcaggctgac cgcgcaccaa 901 ttcgcccttt tcccgctcaa acctttggaa tacggcacgc tttatacggc 951 ggtattcgac tatgtccgca acggacggca cgcgcaggcg aaatggcagt 1001 ttagaacccg aaaacccgat tacccttatt ttgaggtaaa cggcggcgag 1051 acacttgcgg ttagaaaagg cgaaaaatat ttcatccact ggcgcggacg 1101 ctggtgtctg gaagcgtgta cccgttatac ctaccggcgg cagttcggca 1151 acagcctgtc catactccgg cacgaagcgg gcggcattgt cttcagcgtc 1201 agcggaatgg cgggaagccg catcaggctt actccggaag acagcccgga 1251 acgcggtgta accctttatt tgcaggattg a This corresponds to the amino acid sequence <SEQ ID 1550; ORF 537.ng>: g537.pep 1 MKSLFIWLLL LGSAAGVFYH TQΝQSLPAGE LVYPSAPQIR DGGDALHYLΝ 51 RIRTQIGLHA LAHAPVLEΝS ARRHARYLTL ΝPEDGHGEHH PDΝPHYTAQK 101 LTERTRLAGY LYΝGVHEΝIS TEEEAAESSD SDIRTQQRQV DALMSAIYHR 151 LSLLDRHTDE AGAAFVREΝG KTVLVFΝQGΝ GSFERACAKG RRQPEAGRKY 201 YRΝACHΝGAA VYADEAMPVT ELLYTAYPVG GGALPYFYGE RPDPVPEYEI 251 TGΝPASIDFS EAAGKIAMKS FKLYQGKΝEI RPVRVLTAGΝ DPΝGRLTAHQ 301 FALFPLKPLE YGTLYTAVFD YVRKGRHAQA KWQFRTRKPD YPYFEVΝGGE 351 TLAVRKGEKY FIHWRGRWCL EACTRYTYRR QFGΝSLSILR HEAGGIVFSV 401 SGMAGSRIRL TPEDSPERGV TLYLQD * The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1551>: m537.seq (partial) 1 ATGAAATCCC TTTTTATTCG GCTGCTCCTG TTGGGTTCGG CGGCAGGCGT 51 TTTCTACCAT ACCCAAAmCC AATCCCTGCC CGCGGGCGAA CTTGTCTATC 101 CGTCCGCACC GCAAATCAGG GACGGCGGCG ATGCGCTGCA CTACCTCAAC 151 CGCATCCGAG CCCAAATCGG TTTGCACAAG CTGGCACACG CGCCGGTTTT 201 GGAAAACTCC GCCCGCAgGC ACGCAAGCTA CCTCACGCTC AATCCCGAAG 251 ACGGACACGG CGAACACCAT CCCGACAATC CGCACTACAC CGCACAAAAG 301 CTGACCGAAC GCACACGCCT TGCCGGGTAT CTCTACAACG GCGTGCATGA 351 AAACATCAGC ACGGAAGAAG AAGCCGCCGA ATCGTCCGAC AGCGACATCC 401 GCACGCAGCA ACGCCAAGTG GACGGATTAA TGAGCGCAAT CTACCACCGC 451 CTTTCCCTAC TTGACCGCCA TACGGATGAG TCAGGAGCGG CATT ... This corresponds to the amino acid sequence <SEQ ID 1552; ORF 537>: m537.pep (partial) 1 MKSLFIRLLL LGSAAGVFYH TQXQSLPAGE LVYPSAPQIR DGGDALHYLN 51 RIRAQIGLHK LAHAPVLENS ARRHASYLTL NPEDGHGEHH PDNPHYTAQK 101 LTERTRLAGY LYNGVHENIS TEEEAAESSD SDIRTQQRQV DGLMSAIYHR 151 LSLLDRHTDE SGAA... Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 537 shows 95.7% identity over a 164 aa overlap with a predicted ORF (ORF 537.ng) from N gonorrhoeae: m537/g537 10 20 30 40 50 60 m537. pep MKSLFIRLLLLGSAAGVFYHT Q XQSLPAGELVYPSAPQIRDGGDALHYLΝRIRAQIGLHK M M M M M M M I M M M I I I I I I I I I 1 1 I I I I I I I I I 1 1 I I I I I I I I M I I I I g537 MKSLFIWLLLLGSAAGVFYHTQΝQSLPAGELVYPSAPQIRDGGDALHYLΝRIRTQIGLHA 10 20 30 40 50 60 70 80 90 100 110 120 m537. pep LAHAPVLEΝSARRHASYLTLΝPEDGHGEHHPDΝPHYTAQKLTERTRLAGYLYΝGVHEΝIS l l l l l l l l l l l l l l l M M M M M M M M M M M M M M M M M M M M M M g537 LAHAPVLENSARRHARYLTLΝPEDGHGEHHPDΝPHYTAQKLTERTRLAGYLYΝGVHEΝIS 70 80 90 100 110 120 130 140 150 160 m537. pep TEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDESGAA MMMMMMMMMMIMMMIMMMMMMMM g537 TEEEAAESSDSDIRTQQRQVDALMSAIYHRLSLLDRHTDEAGAAFVRENGKTVLVFNQGN 130 140 150 160 170 180 g537 GSFERACAKGRRQPEAGRKYYRNACHNGAAVYADEAMPVTELLYTAYPVGGGALPYFYGE 190 200 210 220 230 240 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 1553>: a537. seq 1 ATGAAATCCC TTTTTATTCG GCTGCTCCTG TTGGGTTCGG CGGCCGGCGT 51 TTTCTATCAT ACCCAAAACC AATCCCTGCC CGCGGGCGAA CTTGTCTATC 101 CGTCCGCACC GCAAATCAGG GACGGCGGCG ATGCGCTGCA CTACCTCAAC 151 CGCATCCGCG CCCAAATCGG TTTGCACAAG CTGGCACACG CGCCGGTTTT 201 GGAAAATTCC GCCCGCAGGC ACGCACGCTA TCTCACGCTC AATCCCGAAG 251 ACGGACACGG CGAACACCAT CCCGACAATC CGCACTACAC CGCACAAAAG 301 CTGACCGAAC GCACACGCCT TGCCGGGTAT CTCTACAACG GCGTGCATGA 351 AAACATCAGC ACGGAAGAGG AAGCCGCCGA ATCGTCCGAC AGCGACATCC 401 GCACGCAGCA ACGCCAAGTG GACGGATTAA TGAGCGCAAT CTACCACCGC 451 CTTTCCCTAC TTGACCGCCA TACGGATGAG GCAGGAGCGG CATTTGTGCG 501 CGAAAACGGT AAAACCGTTC TCGTATTCAA TCAGGGCAAC GGCAGGTTTG 551 AGCGGCATTG CGCCCAAGGC AGAAATCAGC CGGAAGCAGG ACGGAAATAT 601 TACCGCAACG CCTGCCATAA CGGTGCGGTC GTGTACACCG ACGAAGCCAT 651 GCCCGCACAG GAGCTGCTCT ATACAGCCTA TCCCGTCGGC AACGGCGCAC 701 TGCCTTATTT CCACGGCGAG CGTCCAGACC CCGTGCCGGA ATATGAAATC 751 ACGGGCAATC CTGCCAGCAT TGATTTTTCC GAGGCGGCAG GCAAAATTAC 801 GATGAAAAGT TTCAAGCTGT ATCAGGGTAA AAACGAAATC CGCCCCGTCA 851 GGGTTTTAAC CGCCGGCAAC GACCCCAACG GCAGGCTGAC CGCGTACCAA 901 TTCGCGCTTT TCCCGCTCAA GCCTTTGGAA TACGGTACGC TTTATACGGC 951 GGTATTCGAC TATGTCCGCA ACGGACGGCG CGCGCAGGCG AAATGGCAGT 1001 TTAGAACCCG AAAACCCGAT TACCCTTATT TTGAGGTAAA CGGCGGCGAG 1051 ACACTTGCGG TTAGAAAAGG CGAAAAATAT TTCATCCACT GGCGCGGACG 1101 CTGGTGTTTG GAAGCGTGTA CCCGTTATAC CTACCGGCAG CGACCCGGCA 1151 GCCGCCTGTC CATAGGAAGG CACAAGGCGG GCGGCATCGT CTTCAGCGTT 1201 GACGGAATGG CGGGCAGCCG CATCACGCTT GCACCGGAAG GAGAAACGGA 1251 ACGAGGCGTA ACCCTTTATT TACAGGATTG A This corresponds to the amino acid sequence <SEQ ID 1554; ORF 537.a>: a537. pep 1 MKSLFIRLLL LGSAAGVFYH TQNQSLPAGE LVYPSAPQIR DGGDALHYLN 51 RIRAQIGLHK LAHAPVLENS ARRHARYLTL NPEDGHGEHH PDNPHYTAQK 101 LTERTRLAGY LYNGVHENIS TEEEAAESSD SDIRTQQRQV DGLMSAIYHR 151 LSLLDRHTDE AGAAFVRENG KTVLVFNQGN GRFERHCAQG RNQPEAGRKY 201 YRNACHNGAV VYTDEAMPAQ ELLYTAYPVG NGALPYFHGE RPDPVPEYEI 251 TGNPASIDFS EAAGKITMKS FKLYQGKNEI RPVRVLTAGN DPNGRLTAYQ 301 FALFPLKPLE YGTLYTAVFD YVRNGRRAQA KWQFRTRKPD YPYFEVNGGE 351 TLAVRKGEKY FIHWRGRWCL EACTRYTYRQ RPGSRLSIGR HKAGGIVFSV 401 DGMAGSRITL APEGETERGV TLYLQD* m537/a537 98.2% identity in 164 aa overlap 10 20 30 40 50 60 m537. pep MKSLFIRLLLLGSAAGVFYHTQXQSLPAGELVYPSAPQIRDGGDALHYLNRIRAQIGLHK II II I I I I II I I I I I I I I I I I I I I I II II I I I I I I I I I I II I II I I I I I II I II II I II a537 MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGDALHYLNRIRAQIGLHK 10 20 30 40 50 60 70 80 90 100 110 120 m537. pep LAHAPVLENSARRHASYLTLNPEDGHGEHHPDNPHYTAQKLTERTRLAGYLYNGVHENIS II II II I I II I I II I I I I I II I I I I II II I I I I I II I II I I II I II I I I II I II I II I I a537 LAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLTERTRLAGYLYNGVHENIS 70 80 90 100 110 120 130 140 150 160 m537.pep TEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDESGAA I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I II I I II II I M II a537 TEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDEAGAAFVRENGKTVLVFNQGN 130 140 150 160 170 180 a537 GRFERHCAQGRNQPEAGRKYYRNACHNGAWYTDEAMPAQELLYTAYPVGNGALPYFHGE 190 200 210 220 230 240 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1555>: g538. seq 1 atgtcaggta gaacaggacg gaacagtgcc actcaggcgc aaccggaacg 51 cgtcatgctg gtgggcgtaa tgttggataa agatgatacg ggcagcaatg 101 ccgcccgtct gaacggtttt cagacggcat tggcggaagc cgtcgagctg 151 gtcaaagcgg cgggcggcga ttccgtacgc gtggagactg ccaaacgcga 201 ccgcccgcac actgcgctgt ttgtcggcac gggcaaggcg gcggagctgt 251 cggaagcagt tgccgcagac ggcattgatt tggtcgtatt caaccacgaa 301 cttactccca cgcaggaacg caatttggaa aaaatcctcc aatgccgcgt 351 attggacaga gtggggctga ttctggcgat tttcgcccgc cgcgcccgca 401 cgcaggaagg caggctgcaa gtcgagttgg cgcaattgag ccatttggcg 451 ggacgcttga tacgcggtta cggacatttg caaagccagc gcggcggtat 501 cggcatgaaa gggccgggcg aaaccaaact ggaaaccgac cgccgattaa 551 ccgcccatcg gatcaacgcc ttgaaaaaac agcttgccaa cctcaaaaaa 601 cagcgcgccc tgcgccgcaa gtcccgcgag tcgggcagaa tcaaaacgtt 651 tgcgctggtc ggctatacca atgtcggcaa atccagcctg ttcaaccggc 701 tgaccaagtc gggcatatat gcgaaagacc agcttttcgc cactctcgac 751 acgacggcgc ggcggctgta catcagtccc gcatgcagca ttatcctgac 801 cgataccgtc ggattcgtca gcgatctgcc gcacaaactg atttccgcct 851 tttccgccac cttggaagaa accgtgcaag ccgatgtgct gctgcacgtc 901 gtcgatgctg ccgcccggaa cagcgggcag cagattgaag acgtggaaaa 951 cgtactgcaa gaaatccatg cccacgatat tccgtgcatc aaggtgtaca 1001 acaaaaccga cctgctgccg tctgaagaac aaaacacggg catatggcgc 1051 gacgctgcgg gaaaaattgc cgccgtccgc atttccgttg ctgaaaatac This corresponds to the amino acid sequence <SEQ ID 1556; ORF 538.ng>: g538.pep 1 MSGRTGRNSA TQAQPERVML VGVMLDKDDT GSNAARLNGF QTALAEAVEL 51 VKAAGGDSVR VETAKRDRPH TALFVGTGKA AELSEAVAAD GIDLWFNHE 101 LTPTQERNLE KILQCRVLDR VGLILAIFAR RARTQEGRLQ VELAQLSHLA 151 GRLIRGYGHL QSQRGGIGMK GPGETKLETD RRLTAHRINA LKKQLANLKK 201 QRALRRKSRE SGRIKTFALV GYTNVGKSSL FNRLTKSGIY AKDQLFATLD 251 TTARRLYISP ACSIILTDTV GFVSDLPHKL ISAFSATLEE TVQADVLLHV 301 VDAAARNSGQ QIEDVENVLQ EIHAHDIPCI KVYNKTDLLP SEEQNTGIWR 351 DAAGKIAAVR ISVAENTGID ALREAIAEYC AAAPNTDETE MP* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1557>: m538. seq 1 ATGACAGGCA GAACAGGCGG CAACGGCAGT ACCCAAGCGC AACCCGAACG 51 CGTCATGCTG GTGGGCGTAA TGTTGGACAA AGATGGTACG GGCAGTAGTG 101 CCGCCCGTCT GAACGGTTTT CAGACGGCAT TGGCGGAAGC TGTCGAGCTG 151 GTCAAAGCGG CGGGCGGCGA TTCCGTGCGC GTGGAGACTG CCAAACGCGA 201 CCGTCCGCAC ACCGCGCTGT TTGTCGGCAC GGGCAAGGCG GCGGAGCTGT 251 CAGAAGCAGT TGCCGCAGAC GGCATCGATT TGGTCGTATT CAACCACGAA 301 CTCACGCCCA CGCAGGAACG CAACCTTGAA AAAGAACTsA AATGCCGCGT 351 ATTGGACAGG GTAGGGCTGA TTCTGGCGAT TTTCGCTCGC CGCGCCCGCA 401 CGCAGGAAGG CAGGCTGCAA GTCGAGTTGG CGCAATTGAG CCATTTGGCG 451 GGACGCTTGA TACGCGGTTA CGGCCATCTG CAGAGCCAGC GCGGCGGTAT 501 CGGCATGAAA GGCCCCGGCG AAACCAAACT GGAAACCGAC CGCCGATTGA 551 TCGCCCATCG GATCAATGCC TTGATAAAAC AGCTTGCCAA CCTCAAAAAA 601 CAGCGCGCCC TGCGCCGCAA GTCnCGCGAA TCGGGCACAA TCAAAACGTT 651 TGCGCTGGTC GGCTATACAA ATGTCGGAAA ATCCAGCCTG TTCAACCGGC 701 TGACAAAGTC GGGCATATAT GCAAAGGACA AGCTTAGTCC CGAATGCAGC 751 ATTATCCTGA CCGATACCGT CGGATTCGTn AGCGATCTGC CGCAcAAACT 801 GATTTCCGCC TTTTCgCC.A CGCTGGAAGA AACCGCGCAA GCCGATGTGC 851 TGCTGCACGT CGTCGATGCC GCCGCTCCGA ACAGCGGACA GCAGATTGAA 901 GACGTGGAAA ACGTACTGCA AGAAATCCAT GCCGGCGATA TTCCGTGCAT 951 cAAGGTGTAC AACAAAACCG ACCTGCTGCC GTCTGAAGAA CAAAACACGG 1001 GCATATGGCG CGACGCTGCG GGAAAAATTG CCGCCGTCCG CATTTCCGTT 1051 GCTGAAAATA CCGGTATAGA CGCACTGCGC GAAGCcATTG CCGAGTCTTG 1101 TGCCGCCGCA CCAAACACAG ACGAAACCGA AATGCCATGA This corresponds to the amino acid sequence <SEQ ID 1558; ORF 538>: m538.pep 1 MTGRTGGΝGS TQAQPERVML VGVMLDKDGT GSSAARLΝGF QTALAEAVEL 51 VKAAGGDSVR VETAKRDRPH TALFVGTGKA AELSEAVAAD GIDLWFNHE 101 LTPTQERNLE KELKCRVLDR VGLILAIFAR RARTQEGRLQ VELAQLSHLA 151 GRLIRGYGHL QSQRGGIGMK GPGETKLETD RRLIAHRINA LIKQLANLKK 201 QRALRRKSRE SGTIKTFALV GYTNVGKSSL FNRLTKSGIY AKDKLSPECS 251 IILTDTVGFV SDLPHKLISA FSXTLEETAQ ADVLLHWDA AAPNSGQQIE 301 DVENVLQEIH AGDIPCIKVY NKTDLLPSEE QNTGIWRDAA GKIAAVRISV 351 AENTGIDALR EAIAESCAAA PNTDETEMP* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 538 shows 92.1% identity over a 392 aa overlap with a predicted ORF (ORF 538.ng) from N gonorrhoeae: m538/g538 10 20 30 40 50 60 m538.pep MTGRTGGΝGSTQAQPERVMLVGVMLDKDGTGSSAARLΝGFQTALAEAVELVKAAGGDSVR IMM! -Ml MM I || I M II I II II I II II II I II II I II II 11 g538 MSGRTGRΝSATQAQPERVMLVGVMLDKDDTGSΝAARLΝGFQTALAEAVELVKAAGGDSVR 10 20 30 40 50 60 70 80 90 100 110 120 m538. pep VETAKRDRPHTALFVGTGKAAELSEAVAADGIDLWFΝHELTPTQERΝLEKELKCRVLDR M M M M M M M M M M M I M M M M M M M M M M I M M I M I M M I M g538 VETAKRDRPHTALFVGTGKAAELSEAVAADGIDLWFΝHELTPTQERΝLEKILQCRVLDR 70 80 90 100 110 120 130 140 150 160 170 180 m538. pep VGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHLQSQRGGIGMKGPGETKLETD MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g538 VGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHLQSQRGGIGMKGPGETKLETD 130 140 150 160 170 180 190 200 210 220 230 240 m538. pep RRLIAHRINALIKQLANLKKQRALRRKSRESGTIKTFALVGYTNVGKSSLFNRLTKSGIY III MIIIM MMMMMMMMMM I II I II II II II II II II II I II II II g538 RRLTAHRINALKKQLANLKKQRALRRKSRESGRIKTFALVGYTNVGKSSLFNRLTKSGIY 190 200 210 220 230 240 250 260 270 280 m538. pep AKDKL SPECSIILTDTVGFVSDLPHKLISAFSXTLEETAQADVLLHV IMM II MMMMMMMMMMMM MIMMMMIM g538 AKDQLFATLDTTARRLYISPACSIILTDTVGFVSDLPHKLISAFSATLEETVQADVLLHV 250 260 270 280 290 300 290 300 310 320 330 340 m538. pep VDAAAPNSGQQIEDVENVLQEIHAGDIPCIKVYNKTDLLPSEEQNTGIWRDAAGKIAAVR Mill MMMMMMMMM II I II II II II II I II II I II II II II I II II I II g538 VDAAARNSGQQIEDVENVLQEIHAHDIPCIKVYNKTDLLPSEEQNTGIWRDAAGKIAAVR 310 320 330 340 350 360 350 360 370 380 m538. pep ISVAENTGIDALREAIAESCAAAPNTDETEMPX MMMMMMMMM MMMMMMM g538 ISVAENTGIDALREAIAEYCAAAPNTDETEMPX The following partial DNA sequence was identified in N meningitidis <SEQ ID 1559>: a538. seq 1 ATGACAGGCA GAACAGGCCG CAACGGCAGT ACCCAAGCGC AACCCGAACG 51 CGTCATGCTG GTGGGCGTAA TGTTGGACAA AGATGGTACG GGCAGCAGTG 101 CCACCCGTCT GAACGGTTTT CAGACGGCAT TGGCGGAAGC TGTCGAGCTG 151 GTCAAAGCGG CGGGCGGCGA TTCCGTGCGC GTGGAGACTG CCAAACGCGA 201 CCGTCCGCAC ACCGCGCTGT TTGTCGGCAC GGGCAAGGCG GCGGAGCTGT 251 CGGAAGCAGT TGCCGCAGAC GGCATCGATT TGGTCGTATT CAACCACGAA 301 CTTACGCCCA CGCAGGAACG CAATTTGGAA AAAATCCTCC AATGCCGCGT 351 ATTGGACAGA GTGGGGCTGA TTCTGGCGAT TTTCGCCCGC CGCGCCCGCA 401 CGCAGGAAGG CAGGCTGCAA GTCGAGTTGG CACAATTGAG CCATTTGGCG 451 GGACGCTTGA TACGCGGTTA CGGCCATCTG CAGAGCCAGC GCGGCGGTAT 501 CGGCATGAAA GGCCCCGGCG AAACCAAACT GGAAACCGAC CGCCGATTGA 551 TCGCCCATCG GATCAATGCC TTGAAAAAAC AGCTTGCCAA CCTCAAAAAA 601 CAGCGCGCCC TGCGCCGCAA GTCCCGCGAA TCGGGCACAA TCAAAACGTT 651 TGCGCTGGTC GGCTATACCA ATGTCGGCAA ATCCAGTCTG TTCAACCGGC 701 TGACCAAGTC GGGCATATAT GCGAAAGACC AGCTTTTCGC CACACTCGAC 751 ACGACGGCGC GGCGGCTGTA CATCAGTCCC GAATGCAGCA TTATCCTGAC 801 CGATACCGTC GGATTCGTCA GCGATCTGCC GCACAAACTG ATTTCCGCCT 851 TTTCCGCCAC GCTGGAAGAA ACCGCGCAAG CCGATGTGCT GCTGCACGTC 901 GTCGATGCCG CCGCTCCGAA CAGCGGACAG CAGATTGAAG ACGTGGAAAA 951 CGTACTGCAA GAAATCCATG CCGGCGATAT TCCGTGCATC AAGGTGTACA 1001 ACAAAACCGA CCTGCTGCCG TCTGAAGAAC AAAACACGGG CATATGGCGC 1051 GACGCTGCGG GAAAAATTGC CGCCGTCCGC ATTTCCGTTG CTGAAAATAC 1101 CGGTATAGAC GCACTGCGCG AAGCCATTGC CGAGTATTGT GCCGCCGCAC 1151 CAAACACAGA CGAAACCGAA ATGCCATGA This corresponds to the amino acid sequence <SEQ ID 1560; ORF 538. a>: a538.pep 1 MTGRTGRΝGS TQAQPERVML VGVMLDKDGT GSSATRLΝGF QTALAEAVEL 51 VKAAGGDSVR VETAKRDRPH TALFVGTGKA AELSEAVAAD GIDLWFNHE 101 LTPTQERNLE KILQCRVLDR VGLILAIFAR RARTQEGRLQ VELAQLSHLA 151 GRLIRGYGHL QSQRGGIGMK GPGETKLETD RRLIAHRINA LKKQLANLKK 201 QRALRRKSRE SGTIKTFALV GYTNVGKSSL FNRLTKSGIY AKDQLFATLD 251 TTARRLYISP ECSIILTDTV GFVSDLPHKL ISAFSATLEE TAQADVLLHV 301 VDAAAPNSGQ QIEDVENVLQ EIHAGDIPCI KVYNKTDLLP SEEQNTGIWR 351 DAAGKIAAVR ISVAENTGID ALREAIAEYC AAAPNTDETE MP* m538/a538 94.6% identity in 392 aa overlap 10 20 30 40 50 60 m538. pep MTGRTGGNGSTQAQPERVMLVGVMLDKDGTGSSAARLNGFQTALAEAVELVKAAGGDSVR I I I II I II I I II I I II I I II I I I I II I I I I II I M I I II II I II I II II II I II I I II I a538 MTGRTGRNGSTQAQPERVMLVGVMLDKDGTGSSATRLNGFQTALAEAVELVKAAGGDSVR 10 20 30 40 50 60 70 80 90 100 110 120 m538. pep VETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELTPTQERNLEKELKCRVLDR II I II II I I I II II II II I II I I I I II II I I II I I I I I I I I I I I I I I I I I I I M I I I II a538 VETAKRDRPHTALFVGTGKAAELSEAVAADGIDLWFNHELTPTQERNLEKILQCRVLDR 70 80 90 100 110 120 1 130 140 150 160 170 180 m538. pep VGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHLQSQRGGIGMKGPGETKLETD I I II I I II I II I I M I I I I I I I II I I I I I I I II I I II II I I II II I I II I I II I I I II I I a538 VGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHLQSQRGGIGMKGPGETKLETD 130 140 150 160 170 180 190 200 210 220 230 240 m538. pep RRLIAHRINALIKQLANLKKQRALRRKSRESGTIKTFALVGYTNVGKSSLFNRLTKSGIY II II II I I I I I I II I I I I I I I II I I I I I I I II I I I I I II I I I II I I I II I I I II I II II a538 RRLIAHRINALKKQLANLKKQRALRRKSRESGTIKTFALVGYTNVGKSSLFNRLTKSGIY 190 200 210 220 230 240 250 260 270 280 m538.pep AKDKL SPECSIILTDTVGFVSDLPHKLISAFSXTLEETAQADVLLHV IMM I I I I I I I I I II II I I I I II I II II I II I I I II I I I I II I I I a538 AKDQLFATLDTTARRLYISPECSIILTDTVGFVSDLPHKLISAFSATLEETAQADVLLHV 250 260 270 280 290 300 290 300 310 320 330 340 m538. pep VDAAAPNSGQQIEDVENVLQEIHAGDIPCIKVYNKTDLLPSEEQNTGIWRDAAGKIAAVR II I II II I II II II II I I I I I II I I I II I II II I II I I I I I I I I II II I I I I I II I I II I a538 VDAAAPNSGQQIEDVENVLQEIHAGDIPCIKVYNKTDLLPSEEQNTGIWRDAAGKIAAVR 310 320 330 340 350 360 350 360 370 380 m538. pep ISVAENTGIDALREAIAESCAAAPNTDETEMPX MMMMMMMMM II II II I I I II II I a538 ISVAENTGIDALREAIAEYCAAAPNTDETEMPX 370 380 390 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1561>: g539.seq 1 atggaggatc tgcaggaaat cgggttcgat gtcgccgccg taaaggtagg 51 tcggcagcgc gaacatcatc gtctgcatca tacccagtcc ggcaacggca 101 aggcggacga tgtattgttt gcgttctttt tggttggcgg cttcgatttt 151 ttgcgcgtca tagggtgcgg cggtgtagcc tgtctgccgg attttcaaca 201 gaatgtcgga gaggcggatt ttgccgtcgt cccagacgac gcggcagcgg 251 tgcgtgctgt aattgaggtc gatgcggacg atgccgtctg tgcgcaaaag 301 ctgctgttcg atcagccaga cgcaggcggc gcaggtaatg ccgctgagca 351 tcagcactgc ttcgtgcgtg ccattatggg tttccacaaa gtcggattgg 401 acttcgggca ggtcgtacag gcggatttgg tcgaggattt cttggggcgg 451 cagttcggtt tttttcgcgt cggcggtgcg tcgtttgtaa taactgccca 501 agccggaatc gatgatgctt tgtgcgactg cctgacagcc gacgcagcag 551 gtttcgcggt cttcgccttc gtagcggacg gtcagatgca ggttttcggg 601 aacgtccagc ccgcagtgga aacaggtttt tttcatggca tttcggtttc 651 gtctgtgttt ggtgcggcgg cacaatactc ggcaatggct tcgcgcagtg 701 cgtctatacc ggtattttca gcaacggaaa tgcggacggc ggcaattttt 751 cccgcagcgt cgcgccatat gcccgtgttt tgttcttcag acggcagcag 801 gtcggttttg ttgtacacct tgatgcacgg aatatcgtgg gcatggattt 851 cttgcagtac gttttccacg tcttcaatct gctgcccgct gttccgggcg 901 gcagcatcga cgacgtgcag cagcacatcg gcttgcacgg tttcttccaa 951 ggtggcggaa aaggcggaaa tcagtttgtg cggcagatcg ctgacgaatc 1001 cgacggtatc ggtcaggata atgctgcatg cgggactgat gtacagccgc 1051 cgcgccgtcg tgtcgagagt ggcgaaaagc tggtctttcg catatatgcc 1101 cgacttggtc agccggttga acaggctgga tttgccgaca ttggtatag This corresponds to the amino acid sequence <SEQ ID 1562; ORF 539.ng>: g539.pep 1 MEDLQEIGFD VAAVKVGRQR EHHRLHHTQS GNGKADDVLF AFFLVGGFDF 51 LRVIGCGGVA CLPDFQQNVG EADFAWPDD AAAVRAVIEV DADDAVCAQK 101 LLFDQPDAGG AGNAAEHQHC FVRAIMGFHK VGLDFGQ Q ADLVEDFLGR 151 QFGFFRVGGA SFVITAQAGI DDALCDCLTA DAAGFAVFAF VADGQMQVFG 201 NVQPAVETGF FHGISVSSVF GAAAQYSAMA SRSASIPVFS ATEMRTAAIF 251 PAASRHMPVF CSSDGSRSVL LYTLMHGISW AWISCSTFST SSICCPLFRA 301 AASTTCSSTS ACTVSSKVAE KAEISLCGRS LTNPTVSVRI MLHAGLMYSR 351 RAWSRVAKS WSFAYMPDLV SRLNRLDLPT LV* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1563>: m539.seq (partial) 1 ATGGAGGATT TGCAGGAAAT CGGGTTCGAT GTCGCCGCCG TAAAGGTAGG 51 TCGGCAGCGC GAACATCATC GTCTGCATCA TCCCCAGCCC GGCAACGGCG 101 AGGCGGACGA TGTATTGTTT GCGTTCTTTT TGGTTGGCGG CTTCGATTTT 151 TTGCGCGTCA TAGGGTGCGG CGGTGTAGCC TATCTGCCTG ATTTTCAACA 201 GAATGTCGGA AAGGCGGATT TTGCCGTCGT CCCAGACGAc GCGGCaGCgG 251 TGCGTGCTGT AATTGAGGTC GATGCGGACG ATGCCGTCTG TACGCAAAAG 301 CTGCTGTTCG ATCAGCCAGA CGCAGGCGGC GCAGGTGATG CCGCCGAGCA 351 TTAAAACCGC CTCGCGCGTG CCGCCGTGGG TTTCCACAAA GTCGGACTGG 401 ACTTCGGGCA GGTCGTACAG GCGGATTTGG TCGAGGATTT CTTGGGGCGG 451 CAgCTCGGTT TTTTGCGCGT CGGCGGTGCG TTGTTTGTAA TAACTGCCCA 501 AGCCCGCGTC AATAATGCTT TGTGCGACCG CCTGACAGCC GGCGCaCAgG 551 GTTTCGCGGT CTTCGTTTTC GTAACGGACA GTCAGGTGGA GGTGTTCGGG 601 AACATCCAGA CCGCAGTGGA AACAGGTTTT TTTCATGGCA TTTCGGTTTC 651 GTCTGTGTTT GGTGCGGCGG CACAAGACTC GGCAATgGCT TCGCGCAGTG 701 CGTCTATACC GGTATTTTCA GCAACGGAAA TGCGGACGGC GGCAATTTTT 751 CCCGCAGCGT CGCGCCATAT GCCCGTGTTT TGTTCTTCAG ACGGCAGCAG 801 GTCGGTTTTG TTGTACACCT TgATGCACGG AATATCGCCG GCATGGATTT 851 CTTGCAGTAC GTTTTCCACG TCTTCAATCT GCTGTCCGCT GTTCGGAGCG 901 GCGGCATCGA CGACGTGCAG CAGCACATCG GCTTGCGCGG TTTCTTCCAG 951 CGTGGcG.AA AAGGCGGAAA TCAGTTTgTG CGGCAGATCG CTnACGAATC 1001 CGACGGTATC GGTCAGGATA ATGCTGCATT CGGGAC ... This corresponds to the amino acid sequence <SEQ ID 1564; ORF 539>: m539.pep (partial) 1 MEDLQEIGFD VAAVKVGRQR EHHRLHHPQP GΝGEADDVLF AFFLVGGFDF 51 LRVIGCGGVA YLPDFQQΝVG KADFAWPDD AAAVRAVIEV DADDAVCTQK 101 LLFDQPDAGG AGDAAEH*ΝR LARAAVGFHK VGLDFGQWQ ADLVEDFLGR 151 QLGFLRVGGA LFVITAQARV NNALCDRLTA GAQGFAVFVF VTDSQVEVFG 201 NIQTAVETGF FHGISVSSVF GAAAQDSAMA SRSASIPVFS ATEMRTAAIF 251 PAASRHMPVF CSSDGSRSVL LYTLMHGISP AWISCSTFST SSICCPLFGA 301 AASTTCSSTS ACAVSSSVAX KAEISLCGRS LTNPTVSVRI MLHSG.... Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 539 shows 89% identity over a 345 aa overlap with a predicted ORF (ORF 539.ng) from N gonorrhoeae: m539/g539 10 20 30 40 50 60 m539. ep MEDLQEIGFDVAAVKVGRQREHHRLHHPQPGΝGEADDVLFAFFLVGGFDFLRVIGCGGVA MUM II llll II II I I I Mill III I I I h I I I II I I I I I I I I I I I I II I I M I I I g539 MEDL Q EIGFDVAAVKVGR Q REHHRLHHTQSGΝGKADDVLFAFFLVGGFDFLRVTGCGGVA 10 20 30 40 50 60 70 80 90 100 110 120 m539.pep YLPDFQQNVGKADFAWPDDAAAVRAVIEVDADDAVCTQKLLFDQPDAGGAGDAAEHXNR I I I I I I I I I : I I I I I I I I I I II I I I I I I I II I I I I I : I I I I I I I I I II I I I : I I I I : g539 CLPDFQQNVGEADFAWPDDAAAVRAVIEVDADDAVCAQKLLFDQPDAGGAGNAAEHQHC 70 80 90 100 110 120 130 140 150 160 170 180 m539. ep LARAAVGFHKVGLDFGQWQADLVEDFLGRQLGFLRVGGALFVITAQARVNNALCDRLTA : : I I : I I I I II I I I I I I I I I I I II I I I I I I : I I :| I I I I MIIIM : : : I II I III g539 FVRAIMGFHKVGLDFGQWQADLVEDFLGRQFGFFRVGGASFVITAQAGIDDALCDCLTA 130 140 150 160 170 180 190 200 210 220 230 240 m539.pep GAQGFAVFVFVTDSQVEVFGNIQTAVETGFFHGISVSSVFGAAAQDSAMASRSASIPVFS I I I I I I : ! I t : I : : I I I I : I I I I I II I I I I I I I I I I I I I I I g539 DAAGFAVFAFVADGQMQVFGNVQPAVETGFFHGISVSSVFGAAAQYSAMASRSASIPVFS 190 200 210 220 230 240 250 260 270 280 290 300 m539.pep ATEMRTAAIFPAASRHMPVFCSSDGSRSVLLYTLMHGISPAWISCSTFSTSSICCPLFGA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I g539 ATEMRTAAIFPAASRHMPVFCSSDGSRSVLLYTLMHGISWATSCSTFSTSSICCPLFRA 250 260 270 280 290 300 310 320 330 340 πι539. ep AASTTCSSTSACAVSSSVAXKAEISLCGRS TNPTVSVRIMLHSG I I I I I I I I I I I I : I I I :| I I I I I I I I I I I I I I I I I I I I I I I I : I g539 AASTTCSSTSACTVSSKVAEKAEISLCGRSLTNPTVSVRIMLHAGLMYSRRAWSRVAKS 310 320 330 340 350 360 g539 WSFAYMPD VSRLNRLDLPTLV 370 380 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1565>: a539. seq 1 ATGGAGGATT TGCAGGAAAT CGGGTTCGAT GTCGCCGCCG TAAAGGTAGG 51 TCGGCAGCGC GAACATCATC GTCTGCATCA TCCCCAGCCC GGCAACGGCG 101 AGGCGGACGA TGTATTGTTT GCGTTCTTTT TGGTTGGCGG CTTCGATTTT 151 TTGCGCGTCA TAGGGTGCGG CGGTGTAGCC TATCTGCCTG ATTTTCAACA 201 GAATGTCGGA AAGGCGGATT TTGCCGTCGT CCCAGACGAC GCGGCAGCGG 251 TGCGTGCTGT AATTGAGGTC GATGCGGACG ATGCCGTCTG TACGCAAAAG 301 CTGCTGTTCG ATCAGCCAGA CGCAGGCGGC GCAGGTGATG CCGCCGAGCA 351 TTAAAACCGC CTCGCGCGTG CCGCCGTGGG TTTCCACAAA GTCGGACTGG 401 ACTTCGGGCA GGTCGTACAG GCGGATTTGG TCGAGGATTT CTTGGGGCGG 451 CAGCTCGGTT TTTTGCGCGT CGGCGGTGCG TTGTTTGTAA TAACTGCCCA 501 AGCCCGCGTC AATAATGCTT TGTGCGACTG CCTGACAACC GGCGCAGCAG 551 GTTTCGCGGT CTTCGTTTTC GTAACGGACG GTCAGATGCA GGTTTTCGGG 601 AACGTCCAGC CCGCAGTGGA AACAGGTTTT TTTCATGGCA TTTCGGTTTC 651 GTCTGTGTTT GGTGCGGCGG CACAATACTC GGCAATGGCT TCGCGCAGTG 701 CGTCTATACC GGTATTTTCA GCAACGGAAA TGCGGACGGC GGCAATTTTT 751 CCCGCAGCGT CGCGCCATAT GCCCGTGTTT TGTTCTTCAG ACGGCAGCAG 801 GTCGGTTTTG TTGTACACCT TGATGCACGG AATATCGCCG GCATGGATTT 851 CTTGCAGTAC GTTTTCCACG TCTTCAATCT GCTGTCCGCT GTTCGGAGCG 901 GCGGCATCGA CGACGTGCAG CAGCACATCG GCTTGCGCGG TTTCTTCCAG 951 CGTGGCGGAA AAGGCGGAAA TCAGTTTGTG CGGCAGATCG CTGACGAATC 1001 CGACGGTATC GGTCAGGATA ATGCTGCATT CGGGACTGAT GTACAGCCGC 1051 CGCGCCGTCG TGTCGAGTGT GGCGAAAAGC TGGTCTTTCG CATATATGCC 1101 CGACTTGGTC AGCCGGTTGA ACAGACTGGA TTTGCCGACA TTGGTATAG This corresponds to the amino acid sequence <SEQ ID 1566; ORF 539. a>: a539.pep 1 MEDLQEIGFD VAAVKVGRQR EHHRLHHPQP GΝGEADDVLF AFFLVGGFDF 51 LRVIGCGGVA YLPDFQQNVG KADFA PDD AAAVRAVIEV DADDAVCTQK 101 LLFDQPDAGG AGDAAEH*NR LARAAVGFHK VGLDFGQWQ ADLVEDFLGR 151 QLGFLRVGGA LFVITAQARV NNALCDCLTT GAAGFAVFVF VTDGQMQVFG 201 NVQPAVETGF FHGISVSSVF GAAAQYSAMA SRSASIPVFS ATEMRTAAIF 251 PAASRHMPVF CSSDGSRSVL LYTLMHGISP AWISCSTFST SSICCPLFGA 301 AASTTCSSTS ACAVSSSVAE KAEISLCGRS LTNPTVSVRI MLHSGLMYSR 351 RAWSSVAKS WSFAYMPDLV SRLNRLDLPT LV* m539/a539 97.1 % identity in 345 aa overlap 10 20 30 40 50 60 m539. pep MEDLQEIGFDVAAVKVGRQREHHRLHHPQPGNGEADDVLFAFFLVGGFDFLRVIGCGGVA II II II II II II I II II II II II II II I I II I I I I I I II II I I I I I I I I I II II I I I I I I a539 MEDLQEIGFDVAAVKVGRQREHHRLHHPQPGNGEADDVLFAFFLVGGFDFLRVIGCGGVA 10 20 30 40 50 60 70 80 90 100 110 120 m539. pep YLPDFQQNVGKADFAWPDDAAAVRAVIEVDADDAVCTQKLLFDQPDAGGAGDAAEHXNR II II II II II II II II II II II I II II II I I I I II I I I II I II I I I I II I II I I I II I I I a539 YLPDFQQNVGKADFAWPDDAAAVRAVIEVDADDAVCTQKLLFDQPDAGGAGDAAEHXNR 70 80 90 100 110 120 130 140 150 160 170 180 m539. pep LARAAVGFHKVGLDFGQWQADLVEDFLGRQLGFLRVGGALFVITAQARVNNALCDRLTA II II I I I II II I II I II II II II II I I II I II II I II II I II II II I I I I I II I I I II: a539 LARAAVGFHKVGLDFGQWQADLVEDFLGRQLGFLRVGGALFVITAQARVNNALCDCLTT 130 140 150 160 170 180 190 200 210 220 230 240 m539.pep GAQGFAVFVFVTDSQVEVFGNIQTAVETGFFHGISVSSVFGAAAQDSAMASRSASIPVFS II II I I II II II M :: I I II : I I II II II II II II II II I II I llllllllllllll a539 GAAGFAVFVFVTDGQMQVFGNVQPAVETGFFHGISVSSVFGAAAQYSAMASRSASIPVFS 190 200 210 220 230 240 250 260 270 280 290 300 m539.pep ATEMRTAAIFPAASRHMPVFCSSDGSRSVLLYTLMHGISPAWISCSTFSTSSICCPLFGA II II II II II II II II II II II I II II I I II II II II II II I II II I I I II I I I I I I II I a539 ATEMRTAAIFPAASRHMPVFCSSDGSRSVLLYTLMHGISPAWISCSTFSTSSICCPLFGA 250 260 270 280 290 300 310 320 330 340 m539.pep AASTTCSSTSACAVSSSVAXKAEISLCGRSLTNPTVSVRIMLHSG I I II II II II II I II II II II I II I I II I I II I I I I I II I I I I I a539 AASTTCSSTSACAVSSSVAEKAEISLCGRSLTNPTVSVRIMLHSGLMYSRRAWSSVAKS 310 320 330 340 350 360 a539 WSFAYMPDLVSRLNRLDLPTLVX 370 380 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1567>: g540. seq 1 atgccgccct cccgacgcgg caacggggtg ttttatcaaa acggcaaact 51 tgccaatgcg gtttccgctt gccgattgcc aaaccggcaa acctttcccg 101 tgccggtgcc gaacccgatg ccgtctgaac cttcagacgg catcgggtgt 151 ttatttgtcc actcggacgg gtgcaggttc gtattgtgtc gattcgtcgc 201 cgtaatacag cacgccgagt ttgacgggga tgcgtccctg cgatttgcgg 251 tgggcgttgg aatcgcgcaa ggaatacgcg cagccgcagt attcctgctg 301 gtagaagttt tcgcgtttgc tgatttcaat catacgcgcg ccgccgccgc 351 ctttgcgcca gttgaagtcc caataggcca catcatcgta aggcgcggcg 401 gcacggtgtc cgcagtcgtt gatttgcgcc atatttttcc agcgtga This corresponds to the amino acid sequence <SEQ ID 1568; ORF 540.ng>: g540.pep 1 MPPSRRGΝGV FYQΝGKLAΝA VSACRLPΝRQ TFPVPVPΝPM PSEPSDGIGC 51 LFVHSDGCRF VLCRFVAVIQ HAEFDGDASL RFAVGVGIAQ GIRAAAVFLL 101 VEVFAFADFN HTRAAAAFAP VEVPIGHIIV RRGGTVSAW DLRHIFPA * The following partial DNA sequence was identified in N meningitidis <SEQ ID 1569>: m540.seq (partial) 1 .. CCGAACCCGA TGCCGTCTGA ACCTTCAGAC GGCATCGGGT GTTTATTTGT 51 CCACCCGGAT GGGGGCAGGT TCGTATTGTG TCGATTCGTC GCCGTAATAC 101 AGCACGCCGA GTTTGATGGG GATTCTGCCC TGTGATTTGC GGTGGGCATT 151 GGAATCCCTC AGGGAATAGG CACAACCGCA ATATTCCTGC TGGTAGAAGT 201 TTTCACGTTT GCTGATTTCA ATCATGCGCG CGCTGCCGCC GCCTTTGCGC 251 CAGTTGAAAT CCCAATACAC CACATCATCG TAAGGCGCGG CGGCGCGGTG 301 TCCGCAGTCG TTGATTTGCG CCATATTTTT CCAGCGTGA This corresponds to the amino acid sequence <SEQ ID 1570; ORF 540>: m540.pep (partial) 1 ..PΝPMPSEPSD GIGCLFVHPD GGRFVLCRFV AVIQHAEFDG DSAL*FAVGI 51 GIPQGIGTTA IFLLVEVFTF ADFΝHARAAA AFAPVEIPIH HIIVRRGGAV 101 SAWDLRHIF PA* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 540 shows 85.7% identity over a 112 aa overlap with a predicted ORF (ORF 540.ng) from N gonorrhoeae: m540/g540 10 20 30 m540.pep PΝPMPSEPSDGIGCLFVHPDGGRFVLCRFV M M M M M M M M M I I l l l l l l l l g540 GΝGVFYQΝGKLAΝAVSACRLPΝRQTFPVPVPΝPMPSEPSDGIGCLFVHSDGCRFVLCRFV 10 20 30 40 50 60 40 50 60 70 80 90 m540.pep AVIQHAEFDGDSALXFAVGIGIPQGIGTTAIFLLVEVFTFADFΝHARAAAAFAPVEIPIH M I M M M I h M I I I I M I M l = M M I I I I I I M I I I I I M I I I I I I I I I M I g540 AVI Q HAEFDGDASLRFAVGVGIAQGIRAAAVFLLVEVFAFADFΝHTRAAAAFAPVEVPIG 70 80 90 100 110 120 100 110 m540.pep HIIVRRGGAVSAWDLRHIFPAX MMMMMMIMMMMM g540 HIIVRRGGTVSAWDLRHIFPAX 130 140 L' estremita 1 Ν-terminale di meningococco e' assente perche ' interviene la fine del contig The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1571>: a540. seq 1 ATGCCGTCCT CCCGACGCGG CAACGGGGTG TTTTATCAAA ACGGCAAACT 51 TGCCAATGCG GTTTCCGATT GCAGATTGCC AAACCGGCAA ACCTTTCCCG 101 TGCCGATGCC GAACCCGATG CCGTCTGAAC CTTCAGACGG CATCGGGTGT 151 TTATTTGTCC ACCCGGATGG GTGCAGGTTC GTATTGTGTC GATTCGTCGC 201 CGTAATACAG CACGCCGAGT TTGATGGGGA TTCTGCCCTG TGATTTGCGG 251 TGGGCGTTGG AATCCCTCAG GGAATAGGCA CAACCGCAAT ATTCCTGCTG 301 GTAGAAGTTT TCACGTTTGC TGATTTCAAT CATACGCGCG CTGCCGCCGC 351 CTTTGCGCCA GTTGAAATCC CAATACACCA CATCATCGTA AGGCGCGGCG 401 GCGCGGCGGC CGCAGTCGTT AATCTGGTTC ATGTTTTTCC A This corresponds to the amino acid sequence <SEQ ID 1572; ORF 540. a>: a540.pep (partial) 1 MPSSRRGΝGV FYQΝGKLAΝA VSDCRLPΝRQ TFPVPMPΝPM PSEPSDGIGC 51 LFVHPDGCRF VLCRFVAVIQ HAEFDGDSAL *FAVGVGIPQ GIGTTAIFLL 101 VEFTFADFΝ HTRAAAAFAP VEIPIHHIIV RRGGAAAAW ΝLVHVFP m540/a540 92.8% identity in 111 aa overlap 10 20 30 m540. pep PNPMPSEPSDGIGCLFVHPDGGRFVLCRFV I I I I I I II II II I II I I I I II llllllll a540 GNGVFYQNGKLANAVSDCRLPNRQTFPVPMPNPMPSEPSDGIGCLFVHPDGCRFVLCRFV 10 20 30 40 50 60 40 50 60 70 80 90 m540. pep AVIQHAEFDGDSALXFAVGIGIPQGIGTTAIFLLVEVFTFADFNHARAAAAFAPVEIPIH I I I II II I I I II I II II II : I II I I II I I I I I II I II I I I II II I II I II II II I I I I a540 AVIQHAEFDGDSALXFAVGVGIPQGIGTTAIFLLVEVFTFADFNHTRAAAAFAPVEIPIH 70 80 90 100 110 120 100 110 m540.pep HIIVRRGGAVSAWDLRHIFPAX I II I I II I I:: I I Ml M a5 0 HIIVRRGGAAAAWNLVHVFP 130 140 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1573>: g542. seq 1 atgccgaaat ggtcgcgcat acggcgttgc agcgtccttt cgctgatgtt 51 cagcgcggct gtcagccggt tgacttggtg tgcgccgccg tcgaacgcgg 101 cattcagggt gcggctgaag tcttcagacg gcatagcgtc tgcttccgcc 151 gtttgccccg ccgccggctc gatgccgtct gaaaccgtgt cccacaaatc 201 cgacagcagc cgcaacacgt ccgcctcgcg gcgcaatgtt tcgcccaaat 251 gcccctttgg gacggtttgc aggcaggatg ccgccaagcc gcgcaggttt 301 gggggcaaat cccatatcct gaccggttcg cggtaa This corresponds to the amino acid sequence <SEQ ID 1574; ORF 542.ng>: g542.pep 1 MPKWSRIRRC SVLSLMFSAA VSRLTWCAPP SΝAAFRVRLK SSDGIASASA 51 VCPAAGSMPS ETVSHKSDSS RΝTSASRRΝV SPKCPFGTVC RQDAAKPRRF 101 GGKSHILTGS R* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1575>: m542. seq 1 ATGCCGAAAT GGTCGCGCAT ACGGCGTTGC AGCGTCCTTT CACTGATGTT 51 CAGCGCGTCT GTCAGCCGGT TGACTTGGTG TGCGCCGTCG GCAAACGCGG 101 CATTTAGGGT GCGGCTGAAG TCTTCAGACG GCATAGCGTC TGCTTCCGCC 151 GTTTGCCCCG CCGCCGGCCC GATGCCGTCT GAAACCGTGT CCCACAAGTC 201 CGACAGCAGC CGCAACACGT CCGCCTCGCG . CGCAATGTT TCGCCCAAAT 251 GCCCCTTTGG GACGGCTTTC AGGCAGGATG CCGCCAAGCC GCGCAGGTTC 301 GGGGGCAAAT CCCATATCCT GACCGGTTCG CGGTAA This corresponds to the amino acid sequence <SEQ ID 1576; ORF 542>: m542.pep 1 MPKWSRIRRC SVLSLMFSAS VSRLTWCAPS AΝAAFRVRLK SSDGIASASA 51 VCPAAGPMPS ETVSHKSDSS RΝTSASRAMF RPΝAPLGRΝV SPKCPFGTAF 101 RQDAAKPRRF GGKSHILTGS R* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 542 shows 93.7% identity over a 111 aa overlap with a predicted ORF (ORF 542.ng) from N gonorrhoeae: m542/g542 10 20 30 40 50 60 m542. pep MPKWSRIRRCSVLSLMFSASVSRLTWCAPSAΝAAFRVRLKSSDGIASASAVCPAAGPMPS MMMMMMMMMMMIMMM M II I II II II II II I I I II I || || | | || g542 MPKWSRIRRCSVLSLMFSAAVSRLTWCAPPSΝAAFRVRLKSSDGIASASAVCPAAGSMPS 10 20 30 40 50 60 70 80 90 100 110 m542. pep ETVSHKSDSSRΝTSASXRΝVSPKCPFGTAFRQDAAKPRRFGGKSHILTGSRX g542 ETVSHKSDSSRNTSASRRNVSPKCPFGTVCRQDAAKPRRFGGKSHILTGSRX 70 80 90 100 110 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1577>: a542 . seq 1 ATGCCGAAAT GGTCGCGCAT ACGGCGTTGC AGCGTCCTTT CGCTGATGTT 51 CAGCGTGTCT GCCAGCCGGT TGACTTGATG TGCGCCGCCG GCAAACGCGG 101 CATTCAGGAT GCGGCTGAAG TCTTCAGACG GCATAGCGTC TGCTTCCGCC 151 GTTTGCCCCG CCGCCGGCCC GATGCCGTCT GAAACCGTGT CCCACAAGTC 201 CGACAGCAGC CGCAACACGT CCGCCTCGCG GCGCAATGTT TCGCCCAAAT 251 GCCCCTTTGG GACGGCTTTC AGGCAGGATG CCGCCAAGCC GCGCAGGTTC 301 GGGGGCAAAT CCCATATCCT GACCGGTTCG CGGTAA This corresponds to the amino acid sequence <SEQ ID 1578; ORF 542.a>: a542.pep 1 MPKWSRIRRC SVLSLMFSVS ASRLT*CAPP AΝAAFRMRLK SSDGIASASA 51 VCPAAGPMPS ETVSHKSDSS RΝTSASRRΝV SPKCPFGTAF RQDAAKPRRF 101 GGKSHILTGS R* m542/a542 94.6% identity in 111 aa overlap 10 20 30 40 50 60 m542. pep MPKWSRIRRCSVLSLMFSASVSRLTWCAPSAΝAAFRVRLKSSDGIASASAVCPAAGPMPS I I I II I I II II II I II II : I : I II I III II II II M I II II II II I II II II II II I I a542 MPKWSRIRRCSVLSLMFSVSASRLTXCAPPAΝAAFRMRLKSSDGIASASAVCPAAGPMPS 10 20 30 40 50 60 70 80 90 100 110 m542.pep ETVSHKSDSSRΝTSASXRΝVSPKCPFGTAFRQDAAKPRRFGGKSHILTGSRX I II II I II II II II I I II II I I II I I II II II II II II I II I II II II I II a542 ETVSHKSDSSRΝTSASRRΝVSPKCPFGTAFRQDAAKPRRFGGKSHILTGSRX 70 80 90 100 110 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 1579>: g543.seq 1 atggtttgtc ggttatttgc cgccgttttt ggctttcaac tcggcaatca 51 gcccgtcgat gcctttggct ttgatgattt cgccgaattg gttgcggtac 101 acggtaacca ggctcgtgcc ttcgatggcg acgttgtagg tacggtattt 151 gccgccgctt tggtaggtgg taaagtccat attgacgggc ttctgaccgg 201 ggatgccgac ttcggcacgg acgacgattt ccttgccgcc cttattgacg 251 atgggattgt ctttgacgtt gacggtcgcg tttttgaatt tcagcatcgt 301 gccggaatag gtgcggatca gcagggtttg aaattctttg gccaacgctt 351 gtttttgcgc gtcggacgcg gtacgccaag ggttgccgac cgccaatgcg 401 gtcatacgtt ggaaatcgaa atagggaacc gcataggctt cggcttttgg 451 gcgtgcagaa gccgcgtcgc cgcttttgag gatggtcaaa acctgtgtgg 501 cgttttggcg gatttgtccc actgcgtcgg ccggggaggc aaatgccatg 551 ccgatgctca aaataccgat gcccaatgcg ctgatgaagg aggatttttt 601 cacgatgtct ttcctgaaaa tggatgtgta tgtttattct gcggcttttt 651 ccgcattgcc gccctcagcg tttttctcgg cgaagctggt catgaattta 701 ccgatcaggt tttccagaac cattgcagaa ctggttacgg agatggtgtc 751 gccggcagca aggttttccg tatcgccgcc ctgctgcagc ccgatgtact 801 gttcgcccaa aagtcccgaa gtcaggattt gcgcggaaac gtcactgctg 851 aactgatact tgccgtccaa atcaaggcgc accctcgcct gataggattt 901 cgggtcaagc ccgatagcgc cgacgcgccc gaccaatacg cctgcggatt 951 tgacgggggc attgaccttc aaaccgccga tgtcgccgaa atcggcataa 1001 acggcgtaag ttttgtccga accgccgaac gccgcgccgc ccgccacgcg 1051 gaaagcgaga aaggcaaccg ccgccgcgcc gatcaagacg aacagtccga 1101 cccaaaattc caatatgttc tttttcatta a This corresponds to the amino acid sequence <SEQ ID 1580; ORF 543.ng>: g543.pep 1 MVCRLFAAVF GFQLGΝQPVD AFGFDDFAEL VAVHGΝQARA FDGDWGTVF 51 AAALVGGKVH IDGLLTGDAD FGTDDDFLAA LIDDGIVFDV DGRVFEFQHR 101 AGIGADQQGL KFFGQRLFLR VGRGTPRVAD RQCGHTLEIE IGNRIGFGFW 151 ACRSRVAAFE DGQNLCGVLA DLSHCVGRGG KCHADAQNTD AQCADEGGFF 201 HDVFPENGCV CLFCGFFRIA ALSVFLGEAG HEFTDQVFQN HCRTGYGDGV 251 AGSKVFRIAA LLQPDVLFAQ KSRSQDLRGN VTAELILAVQ IKAHPRLIGF 301 RVKPDSADAP DQYACGFDGG IDLQTADVAE IGINGVSFVR TAERRAARHA 351 ESEKGNRRRA DQDEQSDPKF QYVLFH* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1581>: m543.seq 1 ATGGTTTGTC GGTTATTTGC CGCCGTTTTT GGCTTTCAAC TCGGCAATCA 51 GTCCGTCCAC GCCTTTCGCT TTGATAATTT CGCCGAATTG GTTGCGGTAC 101 ACGGTAACCA GGCTCGCGCC TTCGATGGCG ACGTTGTAGG TACGGTATTT 151 ACCGCCGCTT TGGTAGGTGG TGAAGTCCAT GTTGACGGGT TTTTGCCCGG 201 GTACGCCGAC TTCGGCGCGG ACGATGATTT CTTTGCCGCC TTTATTGACG 251 ATGGGATTGT CTTTGACGTT GACGTTGGCG TTTTTTAATT TCAGCATCGT 301 GCCGGAATAG GTGCGGATCA GCAGGGTTTG AAATTCTTTG GCCAACGCTT 351 GTTTTTGCGC GTCGGACGCG GTGCGCCAAG GGTTGCCGAC CGCCAATGCG 401 GTCATACGTT GGAAATCGAA ATAGGGAATC GCATAGGCTT CGGCTTTTTG 451 GCGAGCGGTG TTGGCATCGC CGTTTTTTAA GATGCTCAAT ACTTGAGTGG 501 CGTTTTGACG GATTTGGCTT ACCGCGTCGG CAGGGGCGGC AAATGCCATG 551 CCGATGCTCA AAATACCGAT GCCCAATGCG CTGATGAGGG AGGATTTTTT 601 CATGATTAAG TGTCCTAGTT TGAATATGAT GGCATACGTT TATTCGGCGG 651 CTTTTTCCGC ATTGCCGCCG TCGGCATTTT TCTCGGCAAA ACTCGTCATG 701 AATTTGCCGA TAAGGTTTTC CAGAACCATT GCAGAACTGG TTACGGAGAT 751 GGTGTCGCCG GCAGCAAGGT TTTCCGTGTC GCCGCCCTGC TGCAGCCCGA 801 TGTACTGCTC GCCCAAAAGT CCCGAAGTCA GGATTTGCGC GGAAACGTCG 851 CTGCTGAACT GATACTTGCC GTCCAAATCG AGGCGCACCC TCGCCTGATA 901 GGATTTCGGG TCAAGTCCGA TAGCGCCGAC GCGCCCGACC AATACGCCTG 951 CGGATTTGAC GGGGGCATTG ACCTTCAAAC CGCCGATGTC GCCGAAATCG 1001 GCATAAACGG CGTAAGTTTT GTCCGAACCG CCGAACGCCG CACCGCCGGC 1051 CACGCGGAAA GCGAGAAAGG CAACCGCCGC CGCGCCAATC AGGACGAACA 1101 GTCCGACCCA AAATTCCAAT ATGTTCTTCT TCATTAA This corresponds to the amino acid sequence <SEQ ID 1582; ORF 543>: m543.pep 1 MVCRLFAAVF GFQLGΝQSVH AFRFDΝFAEL VAVHGΝQARA FDGDWGTVF 51 TAALVGGEVH VDGFLPGYAD FGADDDFFAA FIDDGIVFDV DVGVFXFQHR 101 AGIGADQQGL KFFGQRLFLR VGRGAPRVAD RQCGHTLEIE IGΝRIGFGFL 151 ASGVGIAVFX DAQYLSGVLT DLAYRVGRGG KCHADAQNTD AQCADEGGFF 201 HDXVSXFEYD GIRLFGGFFR IAAVGIFLGK TRHEFADKVF QNHCRTGYGD 251 GVAGSKVFRV AALLQPDVLL AQKSRSQDLR GNVAAELILA VQIEAHPRLI 301 GFRVKSDSAD APDQYACGFD GGIDLQTADV AEIGINGVSF VRTAERRTAG 351 HAESEKGNRR RANQDEQSDP KFQYVLLH* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 543 shows 84.2% identity over a 379 aa overlap with a predicted ORF (ORF 543. ng) from N gonorrhoeae: m543/g543 10 20 30 40 50 60 m543. pep MVCRLFAAVFGFQLGΝQSVHAFRFDΝFAELVAVHGΝQARAFDGDWGTVFTAALVGGEVH M M M M I M M M M I I I I I M I I 1 1 I I I I I I I I 1 1 I I I I 1 1 I I I M I I I 1 1 M I g543 MVCRLFAAVFGFQLGΝQPVDAFGFDDFAELVAVHGΝQARAFDGDWGTVFAAALVGGKVH 10 20 30 40 50 60 70 80 90 100 110 120 IT1543 . pep VDGFLPGYADFGADDDFFAAFIDDGIVFDVDVGVFXFQHRAGIGADQQGLKFFGQRLFLR MIM I 111 hi II hi hi 11111 I II I M I II II I II I II II I II II II II II g543 IDGLLTGDADFGTDDDFLAALIDDGIVFDVDGRVFEFQHRAGIGADQQGLKFFGQRLFLR 70 80 90 100 110 120 130 140 150 160 170 180 m543 . pep VGRGAPRVADRQCGHTLEIEIGNRIGFGFLASGVGIAVFXDAQYLSGVLTDLAYRVGRGG M M M M M M M M M M M M I M M I A A \ A I M I I - M i l l g543 VGRGTPRVADRQCGHTLEIEIGNRIGFGFWACRSRVAAFEDGQNLCGVLADLSHCVGRGG 130 140 150 160 170 180 190 200 210 220 230 239 m543.pep KCHADAQNTDAQCADEGGFFHDXVSXFEYDG- IRLFGGFFRIAAVGIFLGKTRHEFADKV g543 KCHADAQNTDAQCADEGGFFHDV- -FPENGCVCLFCGFFRIAALSVFLGEAGHEFTDQV 190 200 210 220 230 240 250 260 270 280 290 299 m543.pep FQNHCRTGYGDGVAGSKVFRVAALLQPDVLLAQKSRSQDLRGNVAAELILAVQIEAHPRL MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g543 FQNHCRTGYGDGVAGSKVFRIAALLQPDVLFAQKSRSQDLRGNVTAELILAVQIKAHPRL 240 250 260 270 280 290 300 310 320 330 340 350 359 m543. pep IGFRVKSDSADAPDQYACGFDGGIDLQTADVAEIGINGVSFVRTAERRTAGHAESEKGNR M M M M M M M M M M M M M M M M M M M M M M I M M l l l l l l l l l g543 IGFRVKPDSADAPDQYACGFDGGIDLQTADVAEIGINGVSFVRTAERRAARHAESEKGNR 300 310 320 330 340 350 360 370 379 m543. pep RRANQDEQSDPKFQYVLLHX M I M M M M M M M M I g543 RRADQDEQSDPKFQYVLFHX 360 370 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1583>: a543. seq 1 ATGGCTTATG GATTACTTGC TGCCGTTTΝT AGCCTTCAAC TCGΝCAATCA 51 GTCCGTCCAC GCCTTTCGCT TTGATAATTT CGCCGAATTG GTTGCGGTAC 101 ACGGTAACCA GGCTCGCGCC TTCGATGGCG ACGTTGTAGG TACGGTATTT 151 ACCGCCGCTT TGGTAGGTGG TGAAGTCCAT GTTGACGGGT TTTTGCCCGG 201 ΝΝACGCCGAC TTCGGCGCGG ACGATGATTT CTTTGCCGCC TTTATTGACG 251 ATΝGGATTGT CTTTGACGTT GACGTTGGCG TTTTTTAATT TCAGCATCGT 301 GCCGGAATAG GTGCGGATCA GCAGGGTTTG AAATTCTTTG GCCAACGCTT 351 GTTTTTGCGC GTCGGACGCG GTGCGCCAAG GGTTGCCGAC CGCCAATGCG 401 GTCATACGTT GGAAATCGAA ATAGGGAATC GCATAGGCTT CGGCTTTTTG 451 GCGGGCGGTG TTGGCATCAC CGCTTTTTAA GATGCTCAAT ACTTGAGTGG 501 CGTTTTGACG GATTTGGTTT ACCGCGTCGG CAGGGGCGGC AAATGCCATG 551 CCGATGCTCA AAATACCGAT GCCCAATGCG CTGATGAAGG AGGATTTTTT 601 CATGATTAAG TGTCCTAGTT TGAATATGAT GGCATACGTT TATTCGGCGG 651 CTTTTTCCGC ATTGCCGCCG TCGGCATTTT TCTCGGCAAA ACTCGTCATG 701 AATTTGCCGA TAAGGTTTTC CAGAACCATT GCAGAACTGG TTACGGAGAT 751 GGTGTCGCCG GCAGCAAGGT TTTCCGTGTC GCCGCCCTGC TGCAGCCCGA 801 TGTACTGCTC GCCCAAAAGT CCCGAAGTCA GGATTTGCGC GGAAACGTCG 851 CTGCTGAACT GATACTTGCC GTCCAAATCG AGGCGCACCC TCGCCTGATA 901 GGATTTCGGG TCAAGTCCGA TAGCGCCGAC GCGCCCGACC AATACGCCTG 951 CGGATTTGAC GGGGGCATTG ACCTTCAAAC CGCCGATGTC GCCGAAATCG 1001 GCATAAACGG CGTAAGTTTT GTCCGAACCG CCGAACGCCG CACCGCCGGC 1051 CACGCGGAAA GCGAGAAAGG CAACCGCCGC CGCGCCAATC AGGACGAACA 1101 GTCCGACCCA AAATTCCAAT ATGTTCTTTT TCATTAA This corresponds to the amino acid sequence <SEQ ID 1584; ORF 543. a>: a543.pep 1 MAYGLLAAVX SLQLXΝQSVH AFRFDΝFAEL VAVHGΝQARA FDGDWGTVF 51 TAALVGGEVH VDGFLPGXAD FGADDDFFAA FIDDXIVFDV DVGVF*FQHR 101 AGIGADQQGL KFFGQRLFLR VGRGAPRVAD RQCGHTLEIE IGΝRIGFGFL 151 AGGVGITAF* DAQYLSGVLT DLVYRVGRGG KCHADAQNTD AQCADEGGFF 201 HD*VS*FEYD GIRLFGGFFR IAAVGIFLGK TRHEFADKVF QNHCRTGYGD 251 GVAGSKVFRV AALLQPDVLL AQKSRSQDLR GNVAAELILA VQIEAHPRLI 301 GFRVKSDSAD APDQYACGFD GGIDLQTADV AEIGINGVSF VRTAERRTAG 351 HAESEKGNRR RANQDEQSDP KFQYVLFH* m543/a543 96.0% identity in 378 aa overlap 10 20 30 40 50 60 m5 3. pep MVCRLFAAVFGFQLGNQSVHAFRFDNFAELVAVHGNQARAFDGDWGTVFTAALVGGEVH I : ill : M I I II II II II II II II II II I II II I II I II I II II II II I II II I a543 MAYGLLAAVXSLQLXNQSVHAFRFDNFAELVAVHGNQARAFDGDWGTVFTAALVGGEVH 10 20 30 40 50 60 70 80 90 100 110 120 m543.pep VDGFLPGYADFGADDDFFAAFIDDGIVFDVDVGVFXFQHRAGIGADQQGLKFFGQRLFLR lllllll II I II II II II II II I II II II II I II I I II II I II I I I I II II I I II II I a5 3 VDGFLPGXADFGADDDFFAAFIDDXIVFDVDVGVFXFQHRAGIGADQQGLKFFGQRLFLR 70 80 90 100 110 120 130 140 150 160 170 180 m543.pep VGRGAPRVADRQCGHTLEIEIGNRIGFGFLASGVGIAVFXDAQYLSGVLTDLAYRVGRGG II I I I II I II II II II II II II II II II I II M I I I : M II II I II II II I I : II I II II a543 VGRGAPRVADRQCGHTLEIEIGNRIGFGFLAGGVGITAFXDAQYLSGVLTDLVYRVGRGG 130 140 150 160 170 180 190 200 210 220 230 240 m543.pep KCHADAQNTDAQCADEGGFFHDXVSXFEYDGIRLFGGFFRIAAVGIFLGKTRHEFADKVF II II II I I II I I II II II I II II II II II II I I II I I II I I II II I I I II I I I I I I I I I I a5 3 KCHADAQNTDAQCADEGGFFHDXVSXFEYDGIRLFGGFFRIAAVGIFLGKTRHEFADKVF 190 200 210 220 230 240 250 260 270 280 290 300 m543. pep QNHCRTGYGDGVAGSKVFRVAALLQPDVLLAQKSRSQDLRGNVAAELILAVQIEAHPRLI II I II I II II II II II II I II II I II I II II I II II I II I I II II II I II II I I I II I I I a543 QNHCRTGYGDGVAGSKVFRVAALLQPDVLLAQKSRSQDLRGNVAAELILAVQIEAHPRLI 250 260 270 280 290 300 310 320 330 340 350 360 m543.pep GFRVKSDSADAPDQYACGFDGGIDLQTADVAEIGINGVSFVRTAERRTAGHAESEKGNRR I I I II I II II I II II I II II II I II I I I I I I II II II II I I I II I I I I I I II I I II I I I I a543 GFRVKSDSADAPDQYACGFDGGIDLQTADVAEIGINGVSFVRTAERRTAGHAESEKGNRR 310 320 330 340 350 360 370 379 m543.pep RANQDEQSDPKFQYVLLHX I I I II I II II I II II I M I a543 RANQDEQSDPKFQYVLFHX 370 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1585>: g544. seq 1 atgaaaaaaa tactcaccgc cgccgccgtc gcactgatcg gcatcctcct 51 cgccaccgtc ctcatccccg acagtaaaac cgcgcccgcc ttctccctgc 101 ccgacctgca cggaaaaacc gtttccaacg ccgacctgca aggcaaagtc 151 accctgatta atttttggtt tccctcctgt ccgggttgtg tgagcgaaat 201 gcccaaagtc accaaaacgg caaacgacta caaaaataaa gatttccaag 251 tcctcgccgt tgcccagccc atcgatccga tagaaagcgt ccgccaatac 301 gtcaaagact acggactgcc gtttaccgtc atttatgatg cggacaaagc 351 cgtcggacag gcattcggca cacaggttta tccgacttcc gtccttatcg 401 gcaaaaaagg cgaaatcctc aaaacttatg tcggcgaacc cgatttcggc 451 aaactctacc aagaaatcga taccgcgctg gcgcaatag This corresponds to the amino acid sequence <SEQ ID 1586; ORF 544.ng>: g544.pep 1 MKKILTAAAV ALIGILLATV LIPDSKTAPA FSLPDLHGKT VSΝADLQGKV 51 TLIΝFWFPSC PGCVSEMPKV TKTAΝDYKΝK DFQVLAVAQP IDPIESVRQY 101 VKDYGLPFTV IYDADKAVGQ AFGTQVYPTS VLIGKKGEIL KTYVGEPDFG 151 KLYQEIDTAL AQ* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1587>: m544. seq 1 ATGAwAAAAA TACTCACCGC CGCCGTCGTC GCACTGATCG GCATCCTCCT 51 TGCCATCGTC CTCmTCCCCG ACAGCAAAAC CGCGCCCGCC TTCTCC TGC 101 CCGACCTGCA CGGAAAAACC GTTTCCAACG CCGACCTGCA AGGCAAAGTA 151 ACCCTGATTA ATTTTTGGTT TCCCTCCTGT CCGGGTTGTG TGAGCGA AT 201 GCCCAAAATC ATTAAAACGG CAAATGACTA TAAAA CAAA AACTTCCAAG 251 TACTTGCCGT CGCCCAGCCC ATCGATCCGA TAGAAAGCGT CCGCCAATAT 301 GTCAAAGACT ACGGTTTGCC GTTTACCGTC ATGTATGATG CGGACAAAGC 351 TGTCGGACAG GCGTTCGGCA CACAGGTTTA TCCGACTTCC GTCCTTATCG 401 GCAAATAAGG CGAAATCTTC AAAACCTACG TCGGCGAACC CGATTTCGGC 451 AAACTCTACC AAGAAATCGA TACGCGCGTG GCGCAATAG This corresponds to the amino acid sequence <SEQ ID 1588; ORF 544>: m544.pep 1 MXKILTAAW ALIGILLAIV LXPDSKTAPA FSXPDLHGKT VSΝADLQGKV 51 TLIΝFWFPSC PGCVSXMPKI IKTAΝDYKXK ΝFQVLAVAQP IDPIESVRQY 101 VKDYGLPFTV MYDADKAVGQ AFGTQVYPTS VLIGK*GEIF KTYVGEPDFG 151 KLYQEIDTRV AQ* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 544 shows 90.7% identity over a 162 aa overlap with a predicted ORF (ORF 544.ng) from N gonorrhoeae: m544/g544 10 20 30 40 50 60 m544.pep MXKILTAAWALIGILLAIVLXPDSKTAPAFSXPDLHGKTVSΝADLQGKVTLIΝFWFPSC I MMMMMMMM II 1111111 j 11 II II I II II II II 11 II I II II II I II g544 MKKILTAAAVALIGILLATVLIPDSKTAPAFSLPDLHGKTVSΝADLQGKVTLIΝFWFPSC 10 20 30 40 50 60 70 80 90 100 110 120 m544.pep PGCVSXMPKIIKTAΝDYKXKΝFQVLAVAQPIDPIESVRQYVKDYGLPFTVMYDADKAVGQ Mill MM MIIIM I A I II II I II II II II II II II II I II I II II I II I II g544 PGCVSEMPKVTKTAΝDYKΝKDFQVLAVAQPIDPIESVRQYVKDYGLPFTVIYDADKAVGQ 70 80 90 100 110 120 130 140 150 160 m544.pep AFGTQVYPTSVLIGKXGEIFKTYVGEPDFGKLYQEIDTRVAQX MMMMIMMM II I A II II I II I II II II II I MM g544 AFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTALAQX 130 140 150 160 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1589>: a544. seq 1 ATGAAAAAAA TACTCACCGC CGCCGTCGTC GCACTGATCG GCATCCTCCT 51 TGCCATCGTC CTCATCCCCG ACAGCAAAAC CGCGCCCGCT TTCTCCCTGT 101 CCGAΝCTGCA CGGAAAAAΝC GTTTΝCAACG CCGACCTGCA AGGCΝAAGTT 151 AΝCCTGATTA AΝTTTTGGTT TCCCTCCTGT CCGGGTTGTG TGAGCGAAAT 201 GΝCCAΝAATC ATTAAAACGG CAAATGACTA TAAAAACAAA AACTTCCAAG 251 TCCTTGCCGT CGCCCAGCCC ATCGATCCGA TAGAAAGCGT CCGCCAATAT 301 GTCAAAGACT ACGGTTTGCC GTTTACCGTC ATGTATGATG CGGACAAAGC 351 TGTCGGACAG GCGTTCGGCA CACAGGTTTA TCCGACTTCC GTCCTTATCG 401 GCAAAAAAGG CGAAATCCTC AAAACTTATG TCGGCGAACC CGATTTCGGC 451 AAACTCTACC AAGAAATCGA TACCGCGCTG GCACAATAG This corresponds to the amino acid sequence <SEQ ID 1590; ORF 544.a>: a544.pep 1 MKKILTAAW ALIGILLAIV LIPDSKTAPA FSLSXLHGKX VXΝADLQGXV 51 XLIXFWFPSC PGCVSEMXXI IKTAΝDYKΝK ΝFQVLAVAQP IDPIESVRQY 101 VKDYGLPFTV MYDADKAVGQ AFGTQVYPTS VLIGKKGEIL KTYVGEPDFG 151 KLYQEIDTAL AQ* m544/a544 88.9% identity in 162 aa overlap 10 20 30 40 50 60 m544 . pep MXKILTAAWALIGILLAIVLXPDSKTAPAFSXPDLHGKTVSNADLQGKVTLINFWFPSC I I I I I I I I I I I I I I I I I I I I l l l l l l l l l l M M l l l l l l M M l l l l l l a544 MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLSXLHGKXVXNADLQGXVXLIXFWFPSC 10 20 30 40 50 60 70 80 90 100 110 120 m544.pep PGCVSXMPKI IKTANDYKXKNFQVLAVAQPIDPIESVRQYVKDYGLPFTVMYDADKAVGQ II II I I lllllllll II I I II II I II II II II II II II I II II II I I I II I I II II a544 PGCVSEMXXIIKTANDYKNKNFQVLAVAQPIDPIESVRQYVKDYGLPFTVMYDADKAVGQ 70 80 90 100 110 120 130 140 150 160 m544.pep AFGTQVYPTSVLIGKXGEIFKTYVGEPDFGKLYQEIDTRVAQX lllllllllllllll I I I : II I II II II II I II II II M a544 AFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTALAQX 130 140 150 160 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1591>: g547. seq 1 atgttcgtag ataacggatt taataaaacg gtagcgagtt ttgcccaaat 51 cgtcgaaact ttcgacgtat tcttctttag gaacgattgc gcctttttta 101 cgcagatgaa acagcggtgc ggttgggtct gctcgttggt atatctcgtt 151 gatatattta caagatgcgg cttcgagatt ccgaaccgct cctttaaaga 201 gcttgggctt ttgatacaga taagtctgtc ggaacgtttt aggactaatg 251 ccgaagtcga gatggatgcc cattacttcc ccttactcag aaaatattta 301 aaatttataa tgttacatat agttacaaat attagagttt tttgtgtgtg 351 cgtcaaggaa ttgttgacaa ttttagttaa aaatttgtct ccaaacggaa 401 aaaagcggtt tgttttttgt tgttaa This corresponds to the amino acid sequence <SEQ ID 1592; ORF 547.ng>: g547.pep 1 MFVDΝGFΝKT VASFAQIVET FDVFFFRΝDC AFFTQMKQRC GWVCSLVYLV 51 DIFTRCGFEI PΝRSFKELGL LIQISLSERF RTΝAEVEMDA HYFPLLRKYL 101 KFIMLHIVTΝ IRVFCVCVKE LLTILVKΝLS PΝGKKRFVFC C* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1593>: m547.seq 1 ATGTTCGTAG ATAACGGATT TAATAAAACG GTAGCGAGTT TTGCCCAAAT 51 CGTCGAAACT TTCGACGTAT TCTTCTTTAG GAACGATTGC GCCTTTTTTA 101 CGCAGATGAA ACAGCGGTGC GGTTGGGTCT GCTCGTTGGT ATATCTCGTT 151 GATATCTTTC CAAGATGCGG ATTCGAGATT CCGAACCGCT CCTTTAAAGA 201 GCTTGGGCTT TTGATACAGA TAAGTCTGTC GGAACGTTTT AGGACTAATG 251 CCGAAGTCGA GATGGATGCT CATTACTTCC CCTTACTCAG AAAATATTTA 301 AAATTTATAA TGTTACATAT ATTTACAAAT ATTAAAGTTT TTTwTTGTGT 351 GTGCGTCAAG GAATTGTTGA CAATTTTAGT TAAAAATTTG TCTCCAAACG 401 GAAAAAAGCG GTTTGTTTTT TGTTGTTAA This corresponds to the amino acid sequence <SEQ ID 1594; ORF 547>: m547.pep 1 MFVDΝGFΝKT VASFAQIVET FDVFFFRΝDC AFFTQMKQRC GWVCSLVYLV 51 DIFPRCGFEI PΝRSFKELGL LIQISLSERF RTΝAEVEMDA HYFPLLRKYL 101 KFIMLHIFTΝ IKVFXCVCVK ELLTILVKΝL SPΝGKKRFVF CC* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 547 shows 97.2% identity over a 142 aa overlap with a predicted ORF (ORF 547.ng) from N gonorrhoeae: m547/g547 10 20 30 40 50 60 m547.pep MFVDNGFNKTVASFAQIVETFDVFFFRNBCAFFTQMKQRCGWVCSLVYLVDIFPRCGFEI MMMMMMMMMMMIMMM MMMMMMMMMMMM g547 MFVDNGFNKTVASFAQIVETFDVFFFRNDCAFFTQMKQRCGWVCSLVYLVDIFTRCGFEI 10 20 30 40 50 60 70 80 90 100 110 120 m547. pep PNRSFKELGLLIQISLSERFRTNAEVEMDAHYFPLLRKYLKFIMLHIFTNIKVFXCVCVK MMMMMMIIIMMMMMMMMMMMMMMMM IMMI Mill g547 PNRSFKELGLLIQISLSERFRTNAEVEMDAHYFPLLRKYLKFIMLHIVTNIRVF-CVCVK 70 80 90 100 110 130 140 m547.pep ELLTILVKNLSPNGKKRFVFCCX MMMMMMMMIMMM g547 ELLTILVKNLSPNGKKRFVFCCX 120 130 140 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1595>: a547. seq 1 ATGTTCGTAG ATAACGGATT TAATAAAACG GTAGCGAGTT TTGCCCAAAT 51 CGTCGAAACT TTCGACGTAT TCTTCTTTAG GAACAATTGC ACCTTTTTTA 101 CGCAGATGAA ACAGCGGTGC GGTTGGGTCT GCTCGTTGGT ATATCTCGTT 151 GATATCTTTC CAAGATGCGG CTTCGAGATT CCGAACCGCT CCTTTAAAGA 201 GCTTGGGCTT TTGATACAGA TAAGTCTGTC GGAACGTTTT AGGACTAATG 251 CCGAAGTCGA GATAGATGCT CATTACTTCC CCTTACTCAG AAAATATTTA 301 AAATTTATAA TGTTACATAT ATTTACAAAT ATTAAAGTTT TTTT . TGTGT 351 GTGCGTCAAG GAATTGTTGA CAATTTTAGT T This corresponds to the amino acid sequence <SEQ ID 1596; ORF 547. a>: a547.pep 1 MFVDΝGFΝKT VASFAQIVET FDVFFFRΝΝC TFFTQMKQRC GWVCSLVYLV 51 DIFPRCGFEI PΝRSFKELGL LIQISLSERF RTΝAEVEIDA HYFPLLRKYL 101 KFIMLHIFTΝ IKVFXCVCVK ELLTILV m547/a547 97.6% identity in 127 aa overlap 10 20 30 40 50 60 m547.pep MFVDΝGFΝKTVASFAQIVETFDVFFFRΝDCAFFTQMKQRCGWVCSLVYLVDIFPRCGFEI II II II II II I II II II I II I II II I I I : I M I II I II II I II II II I II II II II II I I a547 MFVDΝGFΝKTVASFAQIVETFDVFFFRΝΝCTFFTQMKQRCGWVCSLVYLVDIFPRCGFEI 10 20 30 40 50 60 70 80 90 100 110 120 m547. pep PΝRSFKELGLLIQISLSERFRTΝAEVEMDAHYFPLLRKYLKFIMLHIFTΝIKVFXCVCVK MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a547 PΝRSFKELGLLIQISLSERFRTΝAEVEIDAHYFPLLRKYLKFIMLHIFTΝIKVFXCVCVK 70 80 90 100 110 120 130 140 m547.pep ELLTILVKNLSPNGKKRFVFCCX lllllll a547 ELLTILV The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1597>: g548. seq 1 atgttttccg taccgcgttc ctttttgccg ggcgttttcg tacttgccgc 51 gcttgccgcc tgcaaacctc aagacaacag tgcggcgcaa gccgcttctt 101 caagtgcatc cgcgccggct gcggaaaatg cggcaaagcc gcaaacgcgc 151 ggtacggata tgcgtaagga agacatcggc ggcgatttca cactgaccga 201 cggcgaaggc aagcctttca gcctgagcga tttgaaaggc aaggtcgtga 251 ttctgtcttt cggctttacg cactgtcccg atgtctgccc gacagggctt 301 ttgacgtaca gcgacacttt gaagcagttg ggcgggcagg ctaaggacgt 351 gaaagtggtg ttcgtcagca tcgatccgga acgcgacacg cctgaaatca 401 tcggcaagta tgccaaacag ttcaatccgg actttatcgg tctgacggca 451 acgggcggcc aaaacctgcc ggtcatcaag cagcaatacc gcgtggtttc 501 tgccaaaatc aatcaaaaag acgacagcga aaactatttg gtcgaccact 551 cttccggtgc gtatcttatc gataaaaacg gtgaggttgc cattttctcg 601 ccttacggaa gcgagccgga aacgattgct gccgatgtaa ggaccctgct 651 ctga This corresponds to the amino acid sequence <SEQ ID 1598; ORF 548.ng>: g548.pep 1 MFSVPRSFLP GVFVLAALAA CKPQDNSAAQ AASSSASAPA AENAAKPQTR 51 GTDMRKEDIG GDFTLTDGEG KPFSLSDLKG KWILSFGFT HCPDVCPTGL 101 LTYSDTLKQL GGQAKDVKW FVSIDPERDT PEIIGKYAKQ FNPDFIGLTA 151 TGGQNLPVIK QQYRWSAKI NQKDDSENYL VDHSSGAYLI DKNGEVAIFS 201 PYGSEPETIA ADVRTLL* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1599>: m548. seq 1 ATGTTTTCCG TACCGCGTTC CTTTTTGCCG GGCGTTTTCG TACTTGCCGC 51 GCTTGCCGCC TGCAAACCTC AAGACAACAG TGCGGCGCAA GTCGCTTCTT 101 CAAGTGCATC CGCGTCGGCT GCGGAAAATG CGGCAAAGCA AnACACGCGC 151 GGTACGGATA TGCGTAAGGA AGACATCGGC GGCGATTTCA CGCTGACCGA 201 CGGCGAAGGC AAGCCTTTCA ACCTGAGCGA TTTGAAAGGC AAGGTCGTGA 251 TTCTGTCTTT CGGCTTTACG CACTGTCCCG ATGTCTGCCC GACAGAGCTT 301 TTGACGTACA GCGACACGTT GAAGCAGTTG GGCGGGCAGG CTAAGGACGT 351 GAAAGTGGTG TTCGTCAGCA TCGATCCGGA ACGCGACACG CCTGAAATCA 401 TCGGCAAGTA TGCCAAACAG TTCAATCCGG ACTTTATCGs TCTGACGGCA 451 ACGGGCGGCC AAAACCTGCC GGTCATCAAG CAGCAATACC GCGTGGTTTC 501 TGCCAAAGTC AATCAAAAmG ACGACAGCGA AAACTATTTG GTCGACCACT 551 CTTCCGGTGC GTATCTCATC GACAAAAACG GTGAGGTTGC CATTTTCTCG 601 CCTTACGGAA GCGAGCCGGA AACGATTGCT GCCGATGTAA GGACCCTGCT 651 CTGA This corresponds to the amino acid sequence <SEQ ID 1600; ORF 548>: m548.pep 1 MFSVPRSFLP GVFVLAALAA CKPQDNSAAQ VASSSASASA AENAAKQXTR 51 GTDMRKEDIG GDFTLTDGEG KPFNLSDLKG KWILSFGFT HCPDVCPTEL 101 LTYSDTLKQL GGQAKDVKW FVSIDPERDT PEIIGKYAKQ FNPDFIXLTA 151 TGGQNLPVIK QQYRWSAKV NQXDDSENYL VDHSSGAYLI DKNGEVAIFS 201 PYGSEPETIA ADVRTLL* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 548 shows 95.9% identity over a 217 aa overlap with a predicted ORF (ORF 548.ng) from N gonorrhoeae: m548/g548 10 20 30 40 50 60 m548.pep MFSVPRSFLPGVFVLAALAACKPQDΝSAAQVASSSASASAAEΝAAKQXTRGTDMRKEDIG MMMMMMMMMMMMMMMMMMM MIIIM 111111 II 1111 g548 MFSVPRSFLPGVFVLAALAACKPQDΝSAAQAASSSASAPAAEΝAAKPQTRGTDMRKEDIG 10 20 30 40 50 60 70 80 90 100 110 120 m548.pep GDFTLTDGEGKPFΝLSDLKGKWILSFGFTHCPDVCPTELLTYSDTLKQLGGQAKDVKW MMMMMMIMIMMMMMIMMIMMM II II II 111 II I II I II II 11 g548 GDFTLTDGEGKPFSLSDLKGKWILSFGFTHCPDVCPTGLLTYSDTLKQLGGQAKDVKW 70 80 90 100 110 120 130 140 150 160 170 180 m548.pep FVSIDPERDTPEIIGKYAKQFΝPDFIXLTATGGQΝLPVIKQQYRWSAKVΝQXDDSEΝYL MMMMMMMMMMMMM II 11 II I II II I II II II 11 II M I g548 FVSIDPERDTPEIIGKYAK Q FΝPDFIGLTATGGQΝLPVIKQQYRWSAKIΝQKDDSEΝYL 130 140 150 160 170 180 190 200 210 m548 . pep VDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLLX M M M M M M M M M I M M M M I M M M M M g548 VDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLLX 190 200 210 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1601>: a548. seq 1 ATGTTTTCCG TACCGCGTTC CTTTTTGCCG GGCGTTTTCG TACTTGCCGC 51 GCTTGCCGCC TGCAAACCTC AAGACAACAG TGCGGCGCAA GTCGCTTCTT 101 CAAGTGCATC CGCGTCGGCT GCGGAAAATG CGGCAAAGCC GCAAACGCGC 151 GGTACGGATA TGCGTAAGGA AGACATCGGC GGCGATTTCA CGCTGACCGA 201 CGGCGAAGGC AAGCCTTTCA ACCTGAGCGA TTTGAAAGGC AAGGTCGTGA 251 TTCTGTCTTT CGGCTTTACG CACTGTCCCG ATGTCTGCCC GACAGAGCTT 301 TTGACGTACA GCGACACGTT GAAGCAGTTG GGCGGGCAGG CTAAGGACGT 351 GAAAGTGGTG TTCGTCAGCA TCGATCCGGA ACGCGACACG CCTGAAATCA 401 TCGGCAAGTA TGCCAAACAG TTCAATCCGG ACTTTATCGG TCTGACGGCA 451 ACGGGCGACC AAAACCTGCC GGTCATCAAG CAGCAATACC GCGTGGTTTC 501 TGCCAAAGTC AATCAAAAAG ACGACAGCGA AAACTATTTG GTCGACCACT 551 CTTCCGGTGC GTATCTCATC GACAAAAACG GTGAGGTTGC CATTTTCTCG 601 CCTTACGGAA GCGAGCCGGA AACGATTGCT GCCGATGTAA GGACCCTGCT 651 CTGA This corresponds to the amino acid sequence <SEQ ID 1602; ORF 548. a>: a548.pep 1 MFSVPRSFLP GVFVLAALAA CKPQDNSAAQ VASSSASASA AENAAKPQTR 51 GTDMRKEDIG GDFTLTDGEG KPFNLSDLKG KWILSFGFT HCPDVCPTEL 101 LTYSDTLKQL GGQAKDVKW FVSIDPERDT PEIIGKYAKQ FNPDFIGLTA 151 TGDQNLPVIK QQYRWSAKV NQKDDSENYL VDHSSGAYLI DKNGEVAIFS 201 PYGSEPETIA ADVRTLL* m548/a548 97.7% identity in 217 aa overlap 10 20 30 40 50 60 m548. pep MFSVPRSFLPGVFVLAALAACKPQDNSAAQVASSSASASAAENAAKQXTRGTDMRKEDIG II II II II II II I II II I I I II II II II II I II II II II II II II I II II II II II I I a548 MFSVPRSFLPGVFVLAALAACKPQDNSAAQVASSSASASAAENAAKPQTRGTDMRKEDIG 10 20 30 40 50 60 70 80 90 100 110 120 m548.pep GDFTLTDGEGKPFNLSDLKGKWILSFGFTHCPDVCPTELLTYSDTLKQLGGQAKDVKW II II II II I II I II I I II I I II I II I II II I I II I II I I II II I I II I I I II I I I I I I I I a548 GDFTLTDGEGKPFNLSDLKGKWILSFGFTHCPDVCPTELLTYSDTLKQLGGQAKDVKW 70 80 90 100 110 120 130 140 150 160 170 180 m548.pep FVSIDPERDTPEIIGKYAKQFNPDFIXLTATGGQNLPVIKQQYRWSAKVNQXDDSENYL II II II II II II I II II II II I II I I II II I II II II II II II II I I II I lllllll a548 FVSIDPERDTPEIIGKYAKQFNPDFIGLTATGDQNLPVIKQQYRWSAKVNQKDDSENYL 130 140 150 160 170 180 190 200 210 m548.pep VDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLLX II II II I II I II II I II I I II II I II II II II II I I II a548 VDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLLX 190 200 210 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 1603>: g550.seq 1 atgataacgg acaggtttca tctctttcat tttccagtat ctttcattta 51 tcaatctgac aacaaaatgc cgcctgaaaa cagttcagac ggcattttaa 101 ccacaaacgg cttacagctt ccattcgccc aacttggcag cgtaagcttc 151 caaatctgca atcggacggg ttgccacgcc gctttccatc gctgctttgg 201 cggcagccgt agcgacgcga ggcagcaggc gggaatcgaa cggagtagga 251 atcaggtatt ccgcgccgaa ttcgaatttc ttaccgtaag cggcaaccac 301 ttcttcggtt acttcttcca tcgccaaatc tgccaaagca tacacgcagg 351 cgcgtttcat ttcttcgttg atggtggttg cgccgacatc caacgcgccc 401 cggaagatga acgggaagca caatacgttg ttcacttggt tcgggaagtc 451 ggagcggccg gtaccgataa ccacgtccgg acgggtttct ttcgccagcg 501 gcggcaggat ttccggattc gggttggcca tggcgaacac gatgggtttt 551 tcgttcatcg tgttcaacat ttcaggcgtc agcaggtttg cgccggagag 601 gcccaagaag atgtctttgc ctttaaccgc atcggcaagt acgcgccggc 651 cgttgtcttc aacggcgtag aattttttgg attcgtccat gcggtctttg 701 tcttcgcggg tttggtaaat cacgcctttg gagttgcaaa cggttacgtt 751 ttcacgtttc aagcccaaat ccagcagttg gttcaggcag gcaatcgcgg 801 cggcacctgc gccggagcac accaaagtcg cttcttcgat tttacggccg 851 gtataacgca gggcgttcaa tacggcggcg gcggtaatga tggccgtgcc 901 gtgctggtca tcatgaaata cggggatttt gcagcgtttg cgtaa This corresponds to the amino acid sequence <SEQ ID 1604; ORF 550.ng>: g550.pep 1 MITDRFHLFH FPVSFIYQSD NKMPPENSSD GILTTNGLQL PFAQLGSVSF 51 QICNRTGCHA AFHRCFGGSR SDARQQAGIE RSRNQVFRAE FEFLTVSGNH 101 FFGYFFHRQI CQSIHAGAFH FFVDGGCADI QRAPEDEREA QYWHLVREV 151 GAAGTDNHVR TGFFRQRRQD FRIRVGHGEH DGFFVHRVQH FRRQQVCAGE 201 AQEDVFAFNR IGKYAPAWF NGVEFFGFVH AVFVFAGLVN HAFGVANGYV 251 FTFQAQIQQL VQAGNRGGTC AGAHQSRFFD FTAGITQGVQ YGGGGNDGRA 301 VLVIMKYGDF AAFA* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1605>: m550.seq (partial) 1 .. GACGGCATCG GCAAGCACGC GCTGGCCGTT GTCTTCAATG GCGTAGAACT 51 GTTTGGACTC GTCCATACGG TCTTTGTCTT CGCGGGTTTG GTAAATCACG 101 CCTTTGGAGT CGCAAACGGT CACGTTTTCG CGTTTCAAGC CCAAATCCAG 151 CAATTGGwTC AAGCAGGCAA TCGCGGCCGC ACCTGCGCCG GAACACACCA 201 AAGTCGCTTC TTCGATTTTA CGGCCGGTAA AACGCA GGC GTTCAATACG 251 GCGGCGGCGG TAATGATGGC CGTGCCGTGC TGGTCGTCGT GGAATACGGG 301 GATTTTGCAG CGTTTGCGTA A This corresponds to the amino acid sequence <SEQ ID 1606; ORF 550>: m550.pep (partial) 1 ..DGIGKHALAV VFΝGVELFGL VHTVFVFAGL VΝHAFGVAΝG HVFAFQAQIQ 51 QLXQAGΝRGR TCAGTHQSRF FDFTAGKTXG VQYGGGGΝDG RAVLVWEYG 101 DFAAFA* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 550 shows % identity over a aa overlap with a predicted ORF (ORF 550.ng) from N gonorrhoeae: m550/g550 10 20 30 m550. pep DGIGKHALAWFΝGVELFGLVHTVFVFAGLVΝ I M M I I I I I I I I M I M I M I I I I I I I I g550 DGFFVHRVQHFRRQQVCAGEAQEDVFAFΝRIGKYAPAWFΝGVEFFGFVHAVFVFAGLVΝ 190 200 210 220 230 240 40 50 60 70 80 90 m550. pep HAFGVAΝGHVFAFQAQIQQLXQAGΝRGRTCAGTHQSRFFDFTAGKTXGVQYGGGGΝDGRA M M M M M I M M M I M M M M M M M M M M M M I M M I M M M M g550 HAFGVAΝGYVFTFQAQIQQLVQAGΝRGGTCAGAHQSRFFDFTAGITQGVQYGGGGΝDGRA 250 260 270 280 290 300 100 m550 . pep VLVWEYGDFAAFAX 1 I II I 1 ■ • • • • ■ 1 \ 1 I I 1 1 I I 1 11 1 I 1I 11 g550 VLVIMKYGDFAAFAX 310 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1607>: a550. seq 1 CTATATCAAT CTGACAGCAA AATGCCGCCT GAAAACAGTT CAGACGGCAT 51 TTTAACCGCA AACGGCTTAC AGCTTCCATT CGCTCAGCTT GGCAGCGTAA 101 GCTTCCAAAT CTGCAATCGG ACGGGTTGCC ACGCCGCTTT CCATCGCTGC 151 TTTGGCGGCA GCCGTAGCAA CGCGCGGCAG CAGGCGGGAA TCGAACGGAG 201 TCGGAATCAG GTATTCCGCG CCGAATTCAA ATTTCTTACC GTAAGCGGCA 251 ACCACTTCTT CGGTTACCTC TTCCATCGCC AAATCCGCCA AAGCATACAC 301 GCAGGCGCGT TTCATTTCTT CGTTGATGGT CGTCGCGCCG ACATCCAACG 351 CACCGCGGAA GATGAACGGG AAGCACAATA CATTGTTCAC TTGGTTCGGG 401 AAGTCGGAGC GGCCGGTACC GATAACCACG TCCGGACGGG TTTCTTTCGC 451 CAGCGGCGGC AGGATTTCCG GATTCGGGTT GGCCATAGCG AACACGATGG 501 GTTTTTCGTT CATGGTGTTC AGTATTTCAG GCGTCAGCAG GTTCGCGCCG 551 GAGAGGCCCA AGAAGATGTC TTTGCCTTTG ACGGCATCGG CAAGCACGCG 601 CTGGCCGTTG TCTTCAATGG CGTAGAACTG TTTGGACTCG TCCATACGGT 651 CTTTGTCTTC GCGGGTTTGG TAAATCACGC CTTTGGAGTC GCAAACGGTC 701 ACGTTTTCGC GTTTCAAGCC CAAATCCAGC AATTGGTTCA AGCAGGCAAT 751 CGCGGCCGCA CCTGCGCCGG AACACACCAA AGTCGCTTCT TCGATTTTAC 801 GGCCGGTAAA ACGCAGGGCG TTCAATACGG CAGCGGCGGT AATGATGGCC 851 GTGCCGTGCT GGTCGTCGTG GAATACGGGG ATTTTGCAGC GTTTGCGTAA This corresponds to the amino acid sequence <SEQ ID 1608; ORF 550. a>: a550.pep 1 LYQSDSKMPP EΝSSDGILTA ΝGLQLPFAQL GSVSFQICΝR TGCHAAFHRC 51 FGGSRSΝARQ QAGIERSRΝQ VFRAEFKFLT VSGΝHFFGYL FHRQIRQSIH 101 AGAFHFFVDG RRADIQRTAE DEREAQYIVH LVREVGAAGT DΝHVRTGFFR 151 QRRQDFRIRV GHSEHDGFFV HGVQYFRRQQ VRAGEAQEDV FAFDGIGKHA 201 LAWFΝGVEL FGLVHTVFVF AGLVΝHAFGV AΝGHVFAFQA QIQQLVQAGΝ 251 RGRTCAGTHQ SRFFDFTAGK TQGVQYGSGG ΝDGRAVLWV EYGDFAAFA* m550/a550 97.2% identity in 106 aa overlap 10 20 30 m550 . pep DGIGKHALAWFΝGVELFGLVHTVFVFAGL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a550 EHDGFFVHGVQYFRRQQVRAGEAQEDVFAFDGIGKHALAWFΝGVELFGLVHTVFVFAGL 170 180 190 200 210 220 40 50 60 70 80 90 m550. pep VΝHAFGVAΝGHVFAFQAQIQQLXQAGΝRGRTCAGTHQSRFFDFTAGKTXGVQYGGGGΝDG II II I II II I I II II II II II I I I I II II II I I II II I II II II I I I I I II I M II II a550 VΝHAFGVAΝGHVFAFQAQIQQLVQAGΝRGRTCAGTHQSRFFDFTAGKTQGVQYGSGGΝDG 230 240 250 260 270 280 100 m550. pep RAVLVWEYGDFAAFAX a550 RAVLVWEYGDFAAFAX 290 300 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 1609>: g552.seq 1 atgaagctga aaaccttgtt attgcccttc gccgcactgg cattgtgtgc 51 caacgcattt gccgccccgc ccggcgacgc gtcgttggca cgttggctgg 101 atacgcagaa tttcgaccgg gatatagaaa aaaatatgat tgaaggcttt 151 aatgccggat ttaaaccgta tgcggacaaa gcccttgccg aaatgccgga 201 agcgaaaaaa gatcaggcgg cagaagcctt taatcgttat cgtgagaatg 251 ttttgaaaga tttgattacg cccgaagtga aacaggctgt ccgcaatacc 301 ttattgaaga atgcccgtga aatatacacg caagaagaaa ttgacggcat 351 gattgccttt tacggttcgc ctgtcggtca gtccgtcgtt gccaaaaatc 401 cgcgcttaat caagaaatcg atgagtgaaa tagcggtatc ttggactgca 451 ttgtcaggga aaatcgcgcg acatcatctg cccgagttta cggaagagtt 501 acggcgcatc atctgcggcg gtatagtgga ttaa This corresponds to the amino acid sequence <SEQ ID 1610; ORF552.ng>: g552.pep 1 MKLKTLLLPF AALALCANAF AAPPGDASLA RWLDTQNFDR DIEKNMIEGF 51 NAGFKPYADK ALAEMPEAKK DQAAEAFNRY RENVLKDLIT PEVKQAVRNT 101 LLKNAREIYT QEEIDGMIAF YGSPVGQSW AKNPRLIKKS MSEIAVSWTA 151 LSGKIARHHL PEFTEELRRI ICGGIVD* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1611>: m552.seq (partial) 1 ..ATTAAACTGA AAACCTTGTT ATTGCCCTTC GCCACGCTGG CATTGTGCAC 51 CAATGCTTTT GCCGCCCCGC CCAGCGACGC GTCGTTGGCG CGTTGGCTGG 101 ATACGCAGAA TTTTGACCGG GATATAGAAA AAAATATGAT TGAGGGCTTT 151 AATGCCGGAT TTAAACCGTA TGCGGACAAA GCCCTTGCCG AAATGCCGGA 201 AGCGAAAAAA GATCAGGCGG CAGAAGCCTT TAACCGTTAT CGTGAGAATG 251 TTTTGAAAGA TTTGATTACG CCCGAAGTGA AACAGGCTGT CCGCAATACT 301 TTATTGAAGA ATGCCCGTGA GATATACACG CAAGAAGAAA TTGACGGCAT 351 GATTGCCTTT TACGGTTCGC CTGTCGGTCA GTCCGTCGTT GCCAAAAATC 401 CGCGCTTAAT CAAGAAATCG ATGAGTGAAA TAGCGGTATC TTGGACTGCA 451 TTGTCAGGGA AAATCGCGCA ACATCATCTG CCCGAGTTTA CGGAAGAGTT 501 GCGGCGCATC ATCTGCGGCG GTAAAAATCC CGATGCGGGC TGTAAACAAG 551 CCGGACAGGT TGGGAAAAGG CATCAGAAAT AA This corresponds to the amino acid sequence <SEQ ID 1612; ORF 552>: m552.pep (partial) 1 ..IKLKTLLLPF ATLALCTΝAF AAPPSDASLA RWLDTQNFDR DIEKNMIEGF 51 NAGFKPYADK ALAEMPEAKK DQAAEAFNRY RENVLKDLIT PEVKQAVRNT 101 LLKNAREIYT QEEIDGMIAF YGSPVGQSW AKNPRLIKKS MSEIAVSWTA 151 LSGKIAQHHL PEFTEELRRI ICGGKNPDAG CKQAGQVGKR HQK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 552 shows 97.1% identity over a 174 aa overlap with a predicted ORF (ORF 552.ng) from N gonorrhoeae: m552/g552 10 20 30 40 50 60 m552.pep IKLKTLLLPFATLALCTΝAFAAPPSDASLARWLDTQΝFDRDIEKΝMIEGFΝAGFKPYADK MMMMMIMMIMMMMMMMMMMMMMMMMMMMMMM g552 MKLKTLLLPFAALALCAΝAFAAPPGDASLARWLDTQΝFDRDIEKΝMIEGFΝAGFKPYADK 10 20 30 40 50 60 70 80 90 100 110 120 m552.pep ALAEMPEAKKDQAAEAFΝRYREΝVLKDLITPEVKQAVRΝTLLKΝAREIYTQEEIDGMIAF MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g552 ALAEMPEAKKDQAAEAFΝRYREΝVLKDLITPEVKQAVRΝTLLKΝARE I YTQEE IDGMI AF 70 80 90 100 110 120 130 140 150 160 170 180 m552 . pep YGSPVGQSWAKΝPRLIKKSMSEIAVSWTALSGKIAQHHLPEFTEELRRI ICGGKNPDAG MMMMMMMMMMMMMMMMMMMMMMMMMMM g552 YGSPVGQSWAKNPRLIKKSMSEIAVSWTALSGKIARHHLPEFTEELRRIICGGIVDX 130 140 150 160 170 190 m552. pep CKQAGQVGKRHQKX The following partial DNA sequence was identified in N meningitidis <SEQ ID 1613>: a552. seq 1 ATTAAACTGA AAACCTTGTT ATTGCCCTTC GCCACGCTGG CATTGTGCAC 51 CAATGCTTTT GCCGCCCCGC CCAGCGACGC GTCGTTGGCG CGTTGGCTGG 101 ATACGCAGAA TTTTGACCGG GATATAGAAA AAAATATGAT TGAGGGCTTT 151 AATGCCGGAT TTAAACCGTA TGCGGACAAA GCCCTTGCCG AAATGCCGGA 201 AGCGAAAAAA GATCAGGCGG CAGAAGCCTT TAACCGTTAT CGTGAGAATG 251 TTTTGAAAGA TTTGATTACG CCCGAAGTGA AACAGGCTGT CCGCAATACT 301 TTATTGAAGA ATGCCCGTGA GATATACACG CAAGAAGAAA TTGACGGCAT 351 GATTGCCTTT TACGGTTCGC CTGTCGGTCA GTCCGTCGTT GCCAAAAATC 401 CGCGCTTAAT CAAGAAATCG ATGAGTGAAA TAGCGGTATC TTGGACTGCA 451 TTGTCAGGGA AAATCGCGCA ACATCATCTG CCCGAGTTTA CGGAAGAGTT 501 GCGGCGCATC ATCTGCGGCG GTAAAAATCC CGATGCGGGC TGTAAACAAG 551 CCGGACAGGT TGGGAAAAGG CATCAGAAAT AA This corresponds to the amino acid sequence <SEQ ID 1614; ORF 552. a>: a552.pep 1 IKLKTLLLPF ATLALCTNAF AAPPSDASLA RWLDTQNFDR DIEKNMIEGF 51 NAGFKPYADK ALAEMPEAKK DQAAEAFNRY RENVLKDLIT PEVKQAVRNT 101 LLKNAREIYT QEEIDGMIAF YGSPVGQSW AKNPRLIKKS MSEIAVSWTA 151 LSGKIAQHHL PEFTEELRRI ICGGKNPDAG CKQAGQVGKR HQK* m552/a552 100.0% identity in 193 aa overlap 10 20 30 40 50 60 m552. pep IKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEKNMIEGFNAGFKPYADK I I I I I I II II I II I I I I I I I I II II II I II II II II I II II II II II II I I I M l M II I a552 IKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEKNMIEGFNAGFKPYADK 10 20 30 40 50 60 70 80 90 100 110 120 m552. pep ALAEMPEAKKD Q AAEAFNRYRENVLKDLITPEVKQAVRNTLLKNAREIYTQEEIDGMIAF II M II II II I II II II II II II I I II I II II I I I II II II I I I I I I I I I M I I I I I M I a552 ALAEMPEAKKD Q AAEAFNRYRENVLKDLITPEVKQAVRNTLLKNAREIYTQEEIDGMIAF 70 80 90 100 110 120 130 140 150 160 170 180 m552. pep YGSPVG Q SWAKNPRLIKKSMSEIAVSWTALSGKIAQHHLPEFTEELRRIICGGKNPDAG I I I I I II I I M I II II I II II I I I I I I II I I I M M I I I M M M M M I I I M M M M a552 YGSPVG Q SWAKNPRLIKKSMSEIAVSWTALSGKIAQHHLPEFTEELRRIICGGKNPDAG 130 140 150 160 170 180 190 m552. pep CKQAGQVGKRHQKX a552 CKQAGQVGKRHQKX 190 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1615>: m552-l.seq 1 TTGAATATTA AACTGAAAAC CTTGTTATTG CCCTTCGCCA CGCTGGCATT 51 GTGCACCAAT GCTTTTGCCG CCCCGCCCAG CGACGCGTCG TTGGCGCGTT 101 GGCTGGATAC GCAGAATTTT GACCGGGATA TAGAAAAAAA TATGATTGAG 151 GGCTTTAATG CCGGATTTAA ACCGTATGCG GACAAAGCCC TTGCCGAAAT 201 GCCGGAAGCG AAAAAAGATC AGGCGGCAGA AGCCTTTAAC CGTTATCGTG 251 AGAATGTTTT GAAAGATTTG ATTACGCCCG AAGTGAAACA GGCTGTCCGC 301 AATACTTTAT TGAAGAATGC CCGTGAGATA TACACGCAAG AAGAAATTGA 351 CGGCATGATT GCCTTTTACG GTTCGCCTGT CGGTCAGTCC GTCGTTGCCA 401 AAAATCCGCG CTTAATCAAG AAATCGATGA GTGAAATAGC GGTATCTTGG 451 ACTGCATTGT CAGGGAAAAT CGCGCAACAT CATCTGCCCG AGTTTACGGA 501 AGAGTTGCGG CGCATCATCT GCGGCGGTAA AAATCCCGAT GCGGGCTGTA 551 AACAAGCCGG ACAGGTTGGG AAAAGGCATC AGAAATAA This corresponds to the amino acid sequence <SEQ ID 1616; ORF 552-l>: m552-l.pep 1 LΝIKLKTLLL PFATLALCTΝ AFAAPPSDAS LARWLDTQΝF DRDIEKΝMIE 51 GFΝAGFKPYA DKALAEMPEA KKDQAAEAFΝ RYREΝVLKDL ITPEVKQAVR 101 ΝTLLKΝAREI YTQEEIDGMI AFYGSPVGQS VVAKΝPRLIK KSMSEIAVSW 151 TALSGKIAQH HLPEFTEELR RIICGGKΝPD AGCKQAGQVG KRHQK* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1617>: a552-l . seq 1 TTGAATATTA AACTGAAAAC CTTGTTATTG CCCTTCGCCA CGCTGGCATT 51 GTGCACCAAT GCTTTTGCCG CCCCGCCCAG CGACGCGTCG TTGGCGCGTT 101 GGCTGGATAC GCAGAATTTT GACCGGGATA TAGAAAAAAA TATGATTGAG 151 GGCTTTAATG CCGGATTTAA ACCGTATGCG GACAAAGCCC TTGCCGAAAT 201 GCCGGAAGCG AAAAAAGATC AGGCGGCAGA AGCCTTTAAC CGTTATCGTG 251 AGAATGTTTT GAAAGATTTG ATTACGCCCG AAGTGAAACA GGCTGTCCGC 301 AATACTTTAT TGAAGAATGC CCGTGAGATA TACACGCAAG AAGAAATTGA 351 CGGCATGATT GCCTTTTACG GTTCGCCTGT CGGTCAGTCC GTCGTTGCCA 401 AAAATCCGCG CTTAATCAAG AAATCGATGA GTGAAATAGC GGTATCTTGG 451 ACTGCATTGT CAGGGAAAAT CGCGCAACAT CATCTGCCCG AGTTTACGGA 501 AGAGTTGCGG CGCATCATCT GCGGCGGTAA AAATCCCGAT GCGGGCTGTA 551 AACAAGCCGG ACAGGTTGGG AAAAGGCATC AGAAATAA This corresponds to the amino acid sequence <SEQ ID 1618; ORF 552-l.a>: a552-l.pep 1 LNIKLKTLLL PFATLALCTN AFAAPPSDAS LARWLDTQNF DRDIEKNMIE 51 GFNAGFKPYA DKALAEMPEA KKDQAAEAFN RYRENVLKDL ITPEVKQAVR 101 NTLLKNAREI YTQEEIDGMI AFYGSPVGQS VVAKNPRLIK KSMSEIAVSW 151 TALSGKIAQH HLPEFTEELR RIICGGKNPD AGCKQAGQVG KRHQK* a552-l/m552-l 100.0% identity in 195 aa overlap 10 20 30 40 50 60 a552-l.pep LNIKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEKNMIEGFNAGFKPYA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m552-l LNIKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEKNMIEGFNAGFKPYA 10 20 30 40 50 60 70 80 90 100 110 120 a552-l .pep DKALAEMPEAKKDQAAEAFNRYRENVLKDLITPEVKQAVRNTLLKNAREIYTQEEIDGMI I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m552-l DKALAEMPEAKKDQAAEAFNRYRENVLKDLITPEVKQAVRNTLLKNAREIYTQEEIDGMI 70 80 90 100 110 120 130 140 150 160 170 180 a552-l.pep AFYGSPVGQΞVVAKNPRLIKKSMSEIAVSWTALSGKIAQHHLPEFTEELRRIICGGKNPD I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m552-l AFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIAQHHLPEFTEELRRIICGGKNPD 130 140 150 160 170 180 190 a552-l .pep AGCKQAGQVGKRHQKX I I I I I I I I I I I I I I I I m552-l AGCKQAGQVGKRHQKX 190 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1619>: g553. seq 1 atggattatc tgcaaaacct gtctttgggc ttgacaaaaa agctgcccgt 51 tatactgcaa acagaagtag cggagtgtgg cttggcatgt ctagcggctg 101 tggccggatt ttatggtttc tatacggatt tgcgcgcact gcgttcaaaa 151 tactgtctgt cacttaaggg tgagaatttg gcagatattg ttcgttttgc 201 tgatgatatg gggctgacgg gacgggcgtt gaggctggat ttagacgaat 251 tgggcagttt gcgcctgccc tgtattctac attgggattt gaatcatttt 301 gtggtgctgg aatcggtatc ttcggacggg gctgccgtca tggatccggc 351 ttcgggacga cgcaaagtca agacggagga aatatcgcgc aagtttacgg 401 gaattgcttt ggaactgtgg ccaaacacgc gtttcgaggc aggggaagaa 451 aagcaggaaa tccgcatcct acccatgttg cgcgggattt ctgggctggg 501 gcggacattg tttcagcttt tggctttggc agcagcaatg gaagtgtttg 551 cttttttaca aaacgtcagc ttcaagatcg gacgtggtga atcgcttgcg 601 ttaatcggac gatcgggctg cggtaaatcg acacttttgg atattttaag 651 cggcaatcta cctcccgaat caggcaaagt catgataaat gggcacgaca 701 tttacagctt accgccacct tttattccgc aatttgagtg cgatggtcaa 751 ggcaggacga tgttttatag tggattaaat ttaaaccggt ag This corresponds to the amino acid sequence <SEQ ID 1620; ORF 553. ng>: g553.pep 1 MDYLQNLSLG LTKKLPVILQ TEVAECGLAC LAAVAGFYGF YTDLRALRSK 51 YCLSLKGENL ADIVRFADDM GLTGRALRLD LDELGSLRLP CILHWDLNHF 101 WLESVSSDG AAVMDPASGR RKVKTEEISR KFTGIALELW PNTRFEAGEE 151 KQEIRILPML RGISGLGRTL FQLLALAAAM EVFAFLQNVS FKIGRGESLA 201 LIGRSGCGKS TLLDILSGNL PPESGKVMIN GHDIYSLPPP FIPQFECDGQ 251 GRTMFYSGLN LNR* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1621>: m553.seq (partial) 1 ATGGATTATT TATCAAGACT GTCCTTTGGA TTTAACAAAA AGCTACCTGT 51 CATTCTGCAA ACAGAAGTTG CTGAATGTGG TTTAGCATGC CTGACATCCA 101 TCTTGTCCTA TTATGGCTTT CACACTGATT TAAGAACGTT ACGCCAAAAA 151 TACACCCTGT CATTAAAGGG CGCAAATCTT GCAGACATCA TGAGATTTGG 201 CAATGAAATG AATTTAACGC CACGAGCTTT GCGTTTAGAG TTAGATGAGC 251 TGTCAAATTT ACAACTACCC TGCATTCTCC ATTGGAACTT AAACCATTTT 301 GTTGTACTTT GTTCCATTTC CAAAGACAGT ATCGTCATTA TGGACCCTGC 351 TGTCGGTATG CGAAAAATCA AAATGGACGA AGTTTCACAA AAATTCACAG 401 GGATTGCCCT AGAATTATTC CCCAATACCC ATTTTGAAGA GAAAAAAGAA 451 ACAAAGAAAA TCAAAATATT ATCTCTATTA AGGGGGGG . T CAGGCTTAAA 501 ACGCTCTTTA ATTCAAATGC TTATATTAGC TATTTCTTTG GAAGTCTTTG 551 CATTG... This corresponds to the amino acid sequence <SEQ ID 1622; ORF 553>: m553.pep (partial) 1 MDYLSRLSFG FΝKKLPVILQ TEVAECGLAC LTSILSYYGF HTDLRTLRQK 51 YTLSLKGAΝL ADIMRFGΝEM ΝLTPRALRLE LDELSΝLQLP CILHWΝLΝHF 101 WLCSISKDS IVIMDPAVGM RKIKMDEVSQ KFTGIALELF PΝTHFEEKKE 151 TKKIKILSLL RGXSGLKRSL IQMLILAISL EVFAL ... Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 553 shows 65.5% identity over a 185 aa overlap with a predicted ORF (ORF 553.ng) from N gonorrhoeae: 10 20 30 40 50 60 g553. pep MDYLQΝLSLGLTKKLPVILQTEVAECGLACLAAVAGFYGFYTDLRALRSKYCLSLKGEΝL I M M I h h M M M M M M M M M I h : : = M I h 1 1 1 h M M I M i l l I I m553 MDYLSRLSFGFΝKKLPVILQTEVAECGLACLTSILSYYGFHTDLRTLRQKYTLSLKGAΝL 10 20 30 40 50 60 70 80 90 100 110 120 g553.pep ADIVRFADDMGLTGRALRLDLDELGSLRLPCILHWDLΝHFWLESVSSDGAAVMDPASGR m553 ADIMRFGΝEMΝLTPRALRLELDELSΝLQLPCILHWΝLΝHFWLCSISKDSIVIMDPAVGM 70 80 90 100 110 120 130 140 150 160 170 180 g553.pep RKVKTEEISRKFTGIALELWPΝTRFEAGEEKQEIRILPMLRGISGLGRTLFQLLALAAAM M M M M M M M M M M M M I A = M M I M M I I I I M M M I I = = m553 RKIKMDEVSQKFTGIALELFPΝTHFEEKKETKKIKILSLLRGXSGLKRSLIQMLILAISL 130 140 150 160 170 180 190 200 210 220 230 240 g553.pep EVFAFLQΝVSFKIGRGESLALIGRSGCGKSTLLDILSGΝLPPESGKVMIΝGHDIYSLPPP 1 1 1 1 = m553 EVFAL The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1623>: a553. seq 1 ATGCCCCATC TGCAAAACCT GTCTTTGGGC TTAAAGAAAA AGCTGCCTGT 51 TATCCTGCAA ACAGAAATAT CAGAATGCGG CTTGGCATGT CTGGCGGCTG 101 TGGCGGGATT TCATGGTTTC CATACGAATT TACGCGCACT GCGTTCAAAA 151 TAC This corresponds to the amino acid sequence <SEQ ID 1624; ORF 553. a>: a553.pep 1 MPHLQNLSLG LKKKLPVILQ TEISECGLAC LAAVAGFHGF HTNLRALRSK 51 Y m553/a553 62.7% identity in 51 aa overlap 10 20 30 40 50 60 m553. pep MDYLSRLSFGFNKKLPVILQTEVAECGLACLTSILSYYGFHTDLRTLRQKYTLSLKGANL I : I : I I : I : II II II I I I :: I I I II II : : : :: : I II I : II : \ I : II a553 MPHLQNLSLGLKKKLPVILQTEISECGLACLAAVAGFHGFHTNLRALRSKY 10 20 30 40 50 70 80 90 100 110 120 m553.pep ADIMRFGNEMNLTPRALRLELDELSNLQLPCILHWNLNHFVVLCSISKDSIVIMDPAVGM The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1625>: g554. seq.. 1 atgacagcac ataaaatcct gcccgtcctt cttcccatca tcttaggcgt 51 ttctcacgca acggctgcat cgcccgcgcc caacagaccg acggtacacg 101 ccgcccccac gctccaaaca cccgaaaccc tcacggcggc acacatcgtt 151 atcgaccttc aaagcaggca gactttatcc gccaaaaaca ccaatacccc 201 tgtcgaaccg gcggcactaa cccaactgat gaccgcatat ttggttttca 251 aaaacatgaa atcgggaaat atccaatctg aagaaaactt aaaaataccc 301 gaatccgcat gggcttcaga aggaagcaga atgtttgtac gtcccggcga 351 tacggtcagc accgacaaac tcttaaaagg catgattgcc ctatgcgcaa 401 acgatgccgc cctaaccctt gccgaccggc tgggcaacgg ctcgattgaa 451 aattttgtgc aacaaatgaa caaagaagcc cgacgcttgg gcatgaagaa 501 caccgtattc aaaaacccga caggcttggg tagagaagga caggtttcca 551 ccgccaaaga cctctccctg ctgtctgaag cattgatgcg cgactttccg 601 gaatattacc cgctgttttc catcaaatcg ttcaagtttg aaaacataga 651 acaaaacaac cgcaatatcc ttttatatag ggacaacaat gtaaacggcc 701 tgaaagccgg gcacacagaa agcggcggct acaaccttgc cgtgtcatac 751 tccggcaacg gcaggcacat ccttgtcatc acactaggtt cggaatcggc 801 ggaaacccgc gcatcggaca acagcaagct gctgaaccgg gcattgcagg 851 ccttcgatac gcccaaaata tatccgaaag gcaaaaccgt tgcccaaatc 901 caaatttccg gaggcagcaa aaaaaccgtc cgcgcaggct tcctcaaaga 951 agcctacatc actctgccac ataaagaagc gaaaatggca gaacagattt 1001 tggaaaccat acagccgatt cccgccccgg taaaaaaagg gcagatttta 1051 ggaaaaatca aaatcaggca aaacggacat accattgccg aaaaagaaat 1101 cgtcgcactg gaaaacgtag aaaaaagaag ccggtggcaa aggctttgga 1151 cgcgtctgac agggcagtaa This corresponds to the amino acid sequence <SEQ ID 1626; ORF 554.ng>: g554.pep.. 1 MTAHKILPVL LPIILGVSHA TAASPAPΝRP TVHAAPTLQT PETLTAAHIV 51 IDLQSRQTLS AKΝTΝTPVEP AALTQLMTAY LVFKΝMKSGΝ IQSEEΝLKIP 101 ESAWASEGSR MFVRPGDTVS TDKLLKGMIA LCAΝDAALTL ADRLGΝGSIE 151 ΝFVQQMΝKEA RRLGMKΝTVF KΝPTGLGREG QVSTAKDLSL LSEALMRDFP 201 EYYPLFSIKS FKFEΝIEQΝΝ RΝILLYRDΝΝ VΝGLKAGHTE SGGYΝLAVSY 251 SGΝGRHILVI TLGSESAETR ASDΝSKLLΝR ALQAFDTPKI YPKGKTVAQI 301 QISGGSKKTV RAGFLKEAYI TLPHKEAKMA EQILETIQPI PAPVKKGQIL 351 GKIKIRQΝGH TIAEKEIVAL EΝVEKRSRWQ RLWTRLTGQ* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1627>: m554. seq.. 1 ATGACAGCAC ATAAAATCCT GCCCGTCCTG CTTTCCATCA TCTTAGGCGT 51 TTCTCACGCA ACGGCTGCAT CGCCCGCGCC CAACAGACCG ACGGTACACG 101 CCGCCCCCAC GTTCCAAACA CCCGAAACCC TCACAGCGGC ACACATCGTT 151 ATCGACCTTC AAAGCAAACA GATTTTATCC GCCAAAAACA TCAATACCCC 201 TGTTGAACCG GCGGCACTAA CCCAACTGAT GACCGCATAT CTGGTTTTCA 251 AAAACATGAA ATCGGGCAAT ATCCAATCTG AAGAAAACTT AAAAATACCC 301 GAATCCGCAT GGGCTTCAGA AGGAAGCAGA ATGTTTGTAC GTCCCGGCGA 351 TACGGTCAGC ACCGACAAAC TCTTAAAAGG CATGATTGCA CTATCCGCAA 401 ACGATGCCGC CCTAACCCTT GCCGGCCGGC TGGGCAACGG CTCGATTGAA 451 AATTTTGTGC AACAAATGAA CAAAGAAGCC CGACGCTTGG GCATGAAGAA 501 CACTGTATTC AAAAACCCGA CAGGCTTGAG TAGAGAAGGA CAGGTTTCCA 551 CCGCCAAAGA CCTCGCCCTG CTGTCTGAAG CATTGATGCG CGACTTTCCG 601 GAATATTACC CGCTGTTTTC CATCAAATCT TTCAAATTCA AAAATATAGA 651 ACAAAACAAC CGCAATATCC TTTTATATAG GGACAACAAT GTAAACGGTC 701 TGAAAGCCGG ACACACAGAA AGCGGCGGCT ACAACCTTGC CGTGTCATAC 751 TCCGGCAACG GCAGGCACAT CCTTGTCATC ACATTGGGTT CGGAATCGGC 801 GGAAACACGC GCATCAGACA ACAGCAAGCT GCTGAACTGG GCATTGCAGG 851 CCTTCGATAC GCCCAAAATA TATCCGAAAG GCAAAACCGT TGCCCAAATC 901 CAAATTTCCG GAGGCAGCAA AAAAACCGTC CGCGCAGGCT TCCTCAAAGA 951 AGCCTACATC ACTCTGCCAC ATAAGGAAGC GAAAATGGCA GAACAAATTC 1001 TAGAAACCAT ACAGCCGATT CCCGCCCCAG TAAAAAAAGG GCAAATTTTA 1051 GGAAAAATCA AAATCAGACA AAACGGATAC ACCATTGCCG AAAAAGAAAT 1101 CGTCGCACTG GAAAATGTAA AAAAAAGAAG CCGGTGGCAA AGGCTTTGGG 1151 CGTGTCTGAC AGGGCAGTAA This corresponds to the amino acid sequence <SEQ ID 1628; ORF 554>: m554.pep.. 1 MTAHKILPVL LSIILGVSHA TAASPAPNRP TVHAAPTFQT PETLTAAHIV 51 IDLQSKQILS AKNINTPVEP AALTQLMTAY LVFKNMKSGN IQSEENLKIP 101 ESAWASEGSR MFVRPGDTVS TDKLLKGMIA LSANDAALTL AGRLGNGSIE 151 NFVQQMNKEA RRLGMKNTVF KNPTGLSREG QVSTAKDLAL LSEALMRDFP 201 EYYPLFSIKS FKFKNIEQNN RNILLYRDNN VNGLKAGHTE SGGYNLAVSY 251 SGNGRHILVI TLGSESAETR ASDNSKLLNW ALQAFDTPKI YPKGKTVAQI 301 QISGGSKKTV RAGFLKEAYI TLPHKEAKMA EQILETIQPI PAPVKKGQIL 351 GKIKIRQNGY TIAEKEIVAL ENVKKRSRWQ RLWACLTGQ* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 554 shows 96.1% identity over a 389 aa overlap with a predicted ORF (ORF 554.ng) from N gonorrhoeae: m554/g554 10 20 30 40 50 60 m554 -pep MTAHKILPVLLSIILGVSHATAASPAPΝRPTVHAAPTFQTPETLTAAHIVIDLQSKQILS I M 1 1 1 I I 1 1 1 M M M M M M M M M M M M I M M M M M M M M M M I I g554 MTAHKILPVLLPIILGVSHATAASPAPΝRPTVHAAPTLQTPETLTAAHIVIDLQSRQTLS 10 20 30 40 50 60 70 80 90 100 110 120 m554. pep AKΝIΝTPVEPAALTQLMTAYLVFKΝMKSGΝIQSEEΝLKIPESAWASEGSRMFVRPGDTVS Ml MMMMMMMMMMMMMMMMMMMMMMMMMMMM g554 AKΝTΝTPVEPAALTQLMTAYLVFKΝMKSGΝIQSEEΝLKIPESAWASEGSRMFVRPGDTVS 70 80 90 100 110 120 130 140 150 160 170 180 m554.pep TDKLLKGMIALSAΝDAALTLAGRLGΝGSIEΝFVQQMΝKEARRLGMKΝTVFKΝPTGLSREG I M I M I M M l l l l l l l l l I I I I I I I I I I I I I I I I I I I I 1 1 1 I I I I I I I I I 1 1 A 1 1 g554 TDKLLKGMIALCAΝDAALTLADRLGΝGSIEΝFVQQMΝKEARRLGMKΝTVFKΝPTGLGREG 130 140 150 160 170 180 190 200 210 220 230 240 m554.pep QVSTAKDLALLSEALMRDFPEYYPLFSIKSFKFKΝIEQΝΝRΝILLYRDΝΝVΝGLKAGHTE I I I I I I I h i 1 1 1 1 1 1 1 1 1 1 1 1 1 1 M 1 1 1 1 1 1 h i I I M l 1 1 1 1 1 1 1 M M I I 1 1 1 1 I I I g554 Q VSTAKDLSLLSEALMRDFPEYYPLFSIKSFKFEΝIEQΝΝRΝILLYRDΝΝVΝGLKAGHTE 190 200 210 220 230 240 250 260 270 280 290 300 m554. pep SGGYΝLAVSYSGΝGRHILVITLGSESAETRASDΝSKLLΝWALQAFDTPKIYPKGKTVAQI g554 SGGYNLAVSYSGNGRHILVITLGSESAETRASDNSKLLNRALQAFDTPKIYPKGKTVAQI 250 260 270 280 290 300 310 320 330 340 350 360 m554.pep QISGGSKKTVRAGFLKEAYITLPHKEAKMAEQILETIQPIPAPVKKGQILGKIKIRQNGY MIMMMMMMMMMMMMMMMMMMMMMMMMMMMIh g554 QISGGSKKTVRAGFLKEAYITLPHKEAKMAEQILETIQPIPAPVKKGQILGKIKIRQNGH 310 320 330 340 350 360 370 380 390 m554.pep TIAEKEIVALENVKKRSRWQRLWACLTGQX II I II II I II II h II 111 II II.: II II I g554 TIAEKEIVALENVEKRSRWQRLWTRLTGQX 370 380 390 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1629>: a554. seq 1 ATGACAGCAC ATAAAATCCT GCCCGTCCTG CTTTCCATCA TCTTAGGCGT 51 TTCTCACGCA ACGGCTGCAT CGCCCGCGCC CAACAGACCG ACGGCACACG 101 CCGCCCCCAC GTTCCAAACA CCCGAAACCC TCACAGCGGC ACACATCGTT 151 ATCGACCTTC AAAGCAAACA GATTTTATCC GCCAAAAACA TCAATACCCC 201 TGTCGAACCG GCGGCACTAA CCCAACTGAT GACCGCATAT CTGGTTTTCA 251 AAAACATGAA ATCGGGAAAT ATCCGATCTG AAGAAAACTT AAAAATACCC 301 GAATCCGCAT GGGCTTCAGA AGGAAGCAGA ATGTTTGTAC GTCCCGGCGA 351 TACGGTCAGC ACCGACAAAC TCTTAAAAGG CATGATTGCA CTATCCGCAA 401 ACGATGCCGC CCTAACCCTT GCCGGCCGGC TGGGCAACGG CTCGATTGAA 451 AATTTTGTGC AACAAATGAA CAAAGAAGCC CGACGCTTGG GCATGAAGAA 501 CACTGTATTC AAAAATCCGA CAGGCTTGAG TAGAGAAGGA CAGGTTTCCA 551 CCGCCAAAGA CCTCGCCCAG CTGTCTGAAG CATTGATGCG CGACTTTCCG 601 GAATATTACC CGCTGTTTTC CATCAAATCT TTCAAATTCA AAAATATAGA 651 GCAAAACAAC CGCAATATCC TTTTATATAG GGACAACAAT GTAAACGGTC 701 TGAAAGCCGG ACACACAGAA AGCGGCGGCT ACAACCTTGC CGTGTCATAC 751 TCCGGCAACG GCAGGCACAT CCTTGTCATC ACATTGGGTT CGGAATCGGC 801 GGAAACACGC GCATCAGACA ACAGCAAGCT GCTGAACTGG GCATTGCAAG 851 CCTTCGATAC GCCCAAAATA TATCCGAAAG GCAAAACCGT TGCCCAAATC 901 CAAATTTCCG GAGGCAGCAA AAAAACCGTC CGCGCAGGCT TCCTCAAAGA 951 AGCCTACATC ACTCTGCCAC ATAAGGAAGC GAAAATGGCA GAACAAATTC 1001 TAGAAACCAT ACAGCCGATT CCCGCCCCAG TAAAAAAAGG GCAAATTTTA 1051 GGAAAAATCA AAATCAGACA AAACGGATAC ACCATTGCCG AAAAAGAAAT 1101 CGTCGCACTG GAAAATGTAA AAAAAAGAAG CCGGTGGCAA AGGCTTTGGG 1151 CGTGTCTGAC AGGGCAGTAA This corresponds to the amino acid sequence <SEQ ID 1630; ORF 554.a>: a554.pep 1 MTAHKILPVL LSIILGVSHA TAASPAPNRP TAHAAPTFQT PETLTAAHIV 51 IDLQSKQILS AKNINTPVEP AALTQLMTAY LVFKNMKSGN IRSEENLKIP 101 ESAWASEGSR MFVRPGDTVS TDKLLKGMIA LSANDAALTL AGRLGNGSIE 151 NFVQQMNKEA RRLGMKNTVF KNPTGLSREG QVSTAKDLAQ LSEALMRDFP 201 EYYPLFSIKS FKFKNIEQNN RNILLYRDNN VNGLKAGHTE SGGYNLAVSY 251 SGNGRHILVI TLGSESAETR ASDNSKLLNW ALQAFDTPKI YPKGKTVAQI 301 QISGGSKKTV RAGFLKEAYI TLPHKEAKMA EQILETIQPI PAPVKKGQIL 351 GKIKIRQNGY TIAEKEIVAL ENVKKRSRWQ RLWACLTGQ* m554/a554 99.2% identity in 389 aa overlap 10 20 30 40 50 60 m554.pep MTAHKILPVLLSIILGVSHATAASPAPNRPTVHAAPTFQTPETLTAAHIVIDLQSKQILS MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a554 MTAHKILPVLLSIILGVSHATAASPAPNRPTAHAAPTFQTPETLTAAHIVIDLQSKQILS 10 20 30 40 50 60 70 80 90 100 110 120 m554. pep AKNINTPVEPAALT Q LMTAYLVFKNMKSGNIQSEENLKIPESAWASEGSRMFVRPGDTVS I I I I I M II I II I II I II II II II II I I I I I: I I I I I II II II II II II I II I II II I I I a554 AKNINTPVEPAALT Q LMTAYLVFKNMKSGNIRSEENLKIPESAWASEGSRMFVRPGDTVS 70 90 100 110 120 130 140 150 160 170 180 m55 .pep TDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEARRLGMKNTVFKNPTGLSREG II I II I I I II II II I I II I I I II I II I II II II I I II II II II I II I I I I II II II II I I a554 TDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEARRLGMKNTVFKNPTGLSREG 130 140 150 160 - 170 180 190 200 210 220 230 240 m554. pep QVSTAKDLALLSEALMRDFPEYYPLFSIKSFKFKNIEQNNRNILLYRDNNVNGLKAGHTE lllllllll I I I I II I II II II II I II II II II I I II II I II I II I I II II I II I I II I a554 QVSTAKDLAQLSEALMRDFPEYYPLFSIKSFKFKNIEQNNRNILLYRDNNVNGLKAGHTE 190 200 210 220 230 240 250 260 270 280 290 300 m554. pep SGGYNLAVSYSGNGRHILVITLGSESAETRASDNSKLLNWALQAFDTPKIYPKGKTVAQI II II II II II I I II II II I II II II II II II II II I II I II II II I II I I II I I I I I II I a554 SGGYNLAVSYSGNGRHILVITLGSESAETRASDNSKLLNWALQAFDTPKIYPKGKTVAQI 250 260 270 280 290 300 310 320 330 340 350 360 m554.pep QISGGSKKTVRAGFLKEAYITLPHKEAKMAEQILETIQPIPAPVKKGQILGKIKIRQNGY I I I I II I II II II I I II II I II II II II II II I II I II I II I I I II II II II II II II I I a554 QISGGSKKTVRAGFLKEAYITLPHKEAKMAEQILETIQPIPAPVKKGQILGKIKIRQNGY 310 320 330 340 350 360 370 380 390 m554. pep TIAEKEIVALENVKKRSRWQRLWACLTGQX II II II II II II I I II II II I II II II II I a554 TIAEKEIVALENVKKRSRWQRLWACLTGQX 370 380 390 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1631>: g556. seq.. 1 atggacaata agaccaaact gcgcttgggc ggcctgattt tactgaccac 51 cgccgtttta agcctcatta tcgtattgat tgtcgattcc tggccgcttg 101 ccatcctgct tgccgccgtc atcgtcgccg ccgctgcggg cggctttgtt 151 tggacatccc gccgacagca acgccagttt atcgaacgtc tgaaaaaatt 201 cgacatcgat cccgaaaaag gcagaatcaa cgaggcaaac ctgcgccgta 251 tgtaccacag cggcggacaa caccagaaag atgcgattac cctgatctgc 301 ctgtcgcaaa aatgttcggt ggacgaggcg cacgctatgt tcaaaaaacg 351 cccgacacgt caggaaatca atcaaatggc ggcaaaacag tcgcgcggtc 401 agaaacgtcc gcaccgttaa This corresponds to the amino acid sequence <SEQ ID 1632; ORF 556.ng>: g556.pep. 1 MDΝKTKLRLG GLILLTTAVL SLIIVLIVDS WPLAILLAAV IVAAAAGGFV 51 WTSRRQQRQF IERLKKFDID PEKGRIΝEAΝ LRRMYHSGGQ HQKDAITLIC 101 LSQKCSVDEA HAMFKKRPTR QEIΝQMAAKQ SRGQKRPHR* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1633>: m556. seq.. 1 ATGGACAATA AGACCAAACT GCGCTTGGGC GGCCTGATTT TACTGACCAC 51 CGCCGTTTTA AGCCTCATTA TCGTATTGAT TGTCGATTCC TGGCCGCTTG 101 CCATCCTGCT TGCAGCCGTC ATTGTCGCTG CCGCTGCGGG CGGTTTTGTT 151 TGGACATCCC GCCGACAGCA ACGCCAGTTT ATCGAACGCC TGAAAAAATT 201 CGACATCGAT CCCGAAAAAG GCAGAATCAA CGAGGCAAAC CTGCGCCGTA 251 TGTACCACAG CGGCGGACAA CACCAGAAAG ATGCGATTAC CCTGATCTGC 301 CTGTCGCAAA AATGTTCGGT GGACGAGGCG CACGCTATGT TCAAAAAACG 351 CCCGACACGT CAGGAAATCA ATCAAATGGC GGCAAAACAG TCGCGCGGTC 401 AGAAACGTCC GCACCGTTAA This corresponds to the amino acid sequence <SEQ ID 1634; ORF 556>: m556.pep.. 1 MDΝKTKLRLG GLILLTTAVL SLIIVLIVDS WPLAILLAAV IVAAAAGGFV 51 WTSRRQQRQF IERLKKFDID PEKGRIΝEAΝ LRRMYHSGGQ HQKDAITLIC 101 LSQKCSVDEA HAMFKKRPTR QEINQMAAKQ SRGQKRPHR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 556 shows 100.0% identity over a 139 aa overlap with a predicted ORF (ORF 556.ng) from N gonorrhoeae: m556/g556 10 20 30 40 50 60 m556.pep MDΝKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAAAAGGFVWTSRRQQRQF II I II II II II I II II I II II II I I II II I I II II II I I I I I II I II II M I I I I I M I I g556 MDΝKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAAAAGGFVWTSRRQQRQF 10 20 30 40 50 60 70 80 90 100 110 120 m556. pep IERLKKFDIDPEKGRIΝEAΝLRRMYHSGGQHQKDAITLICLSQKCSVDEAHAMFKKRPTR MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g556 IERLKKFDIDPEKGRIΝEAΝLRRMYHSGGQHQKDAITLICLSQKCSVDEAHAMFKKRPTR 70 80 90 100 110 120 130 140 m556.pep QEIΝQMAAKQSRGQKRPHRX I II II II I II I II I II II II g556 QEIΝQMAAKQSRGQKRPHRX 130 140 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1635>: a556. seq 1 ATGGACAATA AGACCAAACT GCGCTTGGGC GGCCTGATTT TACTGACCAC 51 CGCCGTTTTA AGCCTCATTA TCGTATTGAT TGTCGATTCC TGGCCGCTTG 101 CCATCCTGCT TGCCGCCGTC ATCGTCGCCG CCGCTGCGGG CGGCTTTGTT 151 TGGACATCCC GCCGACAGCA ACGCCAGTTT ATCGAACGTC TGAAAAAATT 201 CGACATCGAT CCCGAAAAAG GCAGAATCAA CGAGGCAAAC CTGCGCCGTA 251 TGTACCACAG CGGCGGACAA CACCAAAAAG ATGCGATTAC CCTGATCTGC 301 CTGTCGCAAA AATGTTCGGT GGACGAGGCG CACGCTATGT TCAAAAAACG 351 CCCGACACGT CAGGAAATCA ATCAAATGGC GGCAAAACAG TCGCGCGGTC 401 AGAAACGTCC GCACCGTTAA This corresponds to the amino acid sequence <SEQ ID 1636; ORF 556. a>: a556.pep 1 MDΝKTKLRLG GLILLTTAVL SLIIVLIVDS WPLAILLAAV IVAAAAGGFV 51 WTSRRQQRQF IERLKKFDID PEKGRIΝEAΝ LRRMYHSGGQ HQKDAITLIC 101 LSQKCSVDEA HAMFKKRPTR QEIΝQMAAKQ SRGQKRPHR* m556/a556 100.0% identity in 139 aa overlap 10 20 30 40 50 60 m556.pep MDΝKTKLRLGGLILLTTAVLSLIIVLIVDΞWPLAILLAAVIVAAAAGGFVWTΞRRQQRQF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I a556 MDΝKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAAAAGGFVWTSRRQQRQF 10 20 30 40 50 60 70 80 90 100 110 120 m556.pep IERLKKFDIDPEKGRIΝEAΝLRRMYHSGGQHQKDAITLICLΞQKCSVDEAHAMFKKRPTR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a556 IERLKKFDIDPEKGRIΝEAΝLRRMYHΞGGQHQKDAITLICLSQKCSVDEAHAMFKKRPTR 70 80 90 100 110 120 130 140 m556.pep QEIΝQMAAKQSRGQKRPHRX I I I I I I I I I I I I I I I I I I I I a556 QEIΝQMAAKQSRGQKRPHRX 130 140 The following partial DΝA sequence was identified in N. gonorrhoeae <SEQ ID 1637>: g557.seq 1 atgaacaaaa tattccttac tgccgcagcc ttggtgctgg gcgcgtgcgg 51 tttccacctg aaaggtgcag acggcatttc tccgccgctg acctaccgga 101 gctggcacat cgaaggcgga caggcattgc aatttccttt ggaaaccgcg 151 ctgtatcagg cttcgggcag ggtggacgat gctgccggcg cgcagatgac 201 cctgcgtata gacagcgttt cccaaaacaa ggaaacctat accgttaccc 251 gtgcggcagt catcaacgaa tatcttttga tattgacggt tgaagcgcag 301 gtattgaaac gcggcgagcc ggtcggcaaa ccgatgaccg tgtccgtccg 351 ccgcattttg gattatgccg acaacgaaat tttgggcaaa caggaagaag 401 aagaaaccct gtgggcggaa atgcggcagg atgttgccga acagattgtc 451 cgccgcctga cctttctgaa ggcggaatga This corresponds to the amino acid sequence <SEQ ID 1638; ORF 557.ng>: g557.pep.. 1 MNKIFLTAAA LVLGACGFHL KGADGISPPL TYRSWHIEGG QALQFPLETA 51 LYQASGRVDD AAGAQMTLRI DSVSQNKETY TVTRAAVINE YLLILTVEAQ 101 VLKRGEPVGK PMTVSVRRIL DYADNEILGK QEEEETLWAE MRQDVAEQIV 151 RRLTFLKAE* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1639>: m557.seq.. 1 ATGAACAAAC TGTTTCTTAC TGCCGCAGTG CTGATGCTGG GCGCGTGCGG 51 TTTCCACCTG AAAGGTGCAG ACGGCATTTC TCCGCCGCTG ACCTACCGGA 101 GCTGGCACAT CGAAGGCGGA CAGGCATTGC GGTTTCCTTT GGAAACCGCG 151 CTGTATCAGG CTTCGGGCAG GGTGGACGAT GCTGCCGGCG CGCAGATGAC 201 CCTGCGTATA GACAGCGTTT CCCAAAACAA GGAAACCTAC ACCGTTACCC 251 GTGCGGCAGT CATCAACGAA TATCTTTTGA TATTGACGGT TGAAGCGCAG 301 GTATTGAAAC GCGGCGAGCC GGTCGGTAAA CCGATGACCG TGTCCGTCCG 351 CCGCGTCCTT GCTTATGCCG ACAACGAGAT CTTGGGCAAA CAGGAAGAGG 401 AAGCGGCATT GTGGGCGGAA ATGCGGCAGG ATGCCGCCGA ACAGATTGTC 451 CGCCGCCTGA CCTTTCTGAA GGCGGAATGA This corresponds to the amino acid sequence <SEQ ID 1640; ORF 557>: m557.pep.. 1 MΝKLFLTAAV LMLGACGFHL KGADGISPPL TYRSWHIEGG QALRFPLETA 51 LYQASGRVDD AAGAQMTLRI DSVSQNKETY TVTRAAVINE YLLILTVEAQ 101 VLKRGEPVGK PMTVSVRRVL AYADNEILGK QEEEAALWAE MRQDAAEQIV 151 RRLTFLKAE* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 557 shows 94.3% identity over a 159 aa overlap with a predicted ORF (ORF 557.ng) from N gonorrhoeae: m557/g557 10 20 30 40 50 60 m557. pep MΝKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALRFPLETALYQASGRVDD l l h l l l l h h l l l l l l l l l l l l l l l l M I I M I I I I M I I h l M I M M I I I I I I M g557 MΝKIFLTAAALVLGACGFHLKGADGISPPLTYRSWHIEGGQALQFPLETALYQASGRVDD 10 20 30 40 50 60 70 80 90 100 110 120 m557. pep AAGAQMTLRIDSVSQΝKETYTVTRAAVIΝEYLLILTVEAQVLKRGEPVGKPMTVSVRRVL E 1111 II II I i II I M II I II 111 M 11 II M M 11 II M 11 II M M II II 11 i I M M g557 AAGAQMTLRIDSVSQΝKETYTVTRAAVIΝEYLLILTVEAQVLKRGEPVGKPMTVSVRRIL 70 80 90 100 110 120 130 140 150 160 m557.pep AYADΝEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAEX M I M M M M M M I M I I I h I I I 1 1 1 1 1 1 I I 1 1 I I g557 DYADΝEILGKQEEEETLWAEMRQDVAEQIVRRLTFLKAEX 130 140 150 160 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1641 a557. seq 1 ATGAACAAAC TGTTTCTTAC TGCCGCAGTG CTGATGCTGG GCGCGTGCGG 51 TTTCCACCTG AAAGGTGCAG ACGGCATTTC TCCGCCGCTG ACCTACCGGA 101 GCTGGCACAT CGAAGGCGGA CAGGCATTGC AGTTTCCTTT GGAAACCGCG 151 CTGTATCAGG CTTCGGGTAG GGTGGACGAT GCTGCCGGCG CGCAGATGAC 201 CCTGCGTATA GACAGCGTTT CCCAAAACAA GGAAACCTAC ACCGTTACCC 251 GTGCGGCAGT CATCAACGAA TATCTTTTGA TATTGACGGT TGAAGCGCAG 301 GTATTGAAAC GCGGCGAGCC GGTCGGCAAA CCGATGACCG TGTCCGTCCG 351 CCGCGTCCTT GCTTATGCCG ACAACGAGAT CTTGGGCAAA CAGGAAGAGG 401 AAGCGGCATT GTGGGCGGAA ATGCGGCAGG ATGCCGCCGA ACAGATTGTC 451 CGCCGCCTGA CCTTTCTGAA GGCGGAATGA This corresponds to the amino acid sequence <SEQ ID 1642; ORF 557. a>: a557.pep 1 MNKLFLTAAV LMLGACGFHL KGADGISPPL TYRSWHIEGG QALQFPLETA 51 LYQASGRVDD AAGAQMTLRI DSVSQNKETY TVTRAAVINE YLLILTVEAQ 101 VLKRGEPVGK PMTVSVRRVL AYADNEILGK QEEEAALWAE MRQDAAEQIV 151 RRLTFLKAE* m557/a557 99.4% identity in 159 aa overlap 10 20 30 40 50 60 m557. pep MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALRFPLETALYQASGRVDD II II II I II II I II II II II II II I II I I II II II I II II I II : I II II II I I I II II II a557 MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQFPLETALYQASGRVDD 10 20 30 40 50 60 70 80 90 100 110 120 m557. pep AAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVLKRGEPVGKPMTVSVRRVL II II I II II II I II II II II I I II I I I I I II II II I II II I II I I II II II II II I II I I a557 AAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVLKRGEPVGKPMTVSVRRVL 70 80 90 100 110 120 130 140 150 160 m557.pep AYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAEX II II II II I II I II II II II II II I II I I II I II II II II a557 AYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAEX 130 140 150 160 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1643>: g558. seq.. 1 ATGGATGCTT GTTTTTTCGT CATTCCCGCA CAGGCGGGAA TTCGGAGATT 51 CGGGATTGTT TTCAAACGTT CGGGTCGGAT TCTTGCCGGT GCGGGAATGA 101 TGCCCTTATA TACTTTCTCC GAGCTTTATA TGCTTCAACA GGGGACGGCA 151 CATCAAGCAC CGCACTGCGT GTTGCCCGAA CGAGGCTGCC CTCCGATTAG 201 ATTCTATCGC TATAAACAGA CGGGTTTCAA CCGAAAAGGA ATGGGGATAA 251 AGTCCATTTC CGACACCTCT CGGGCGATGC CGTCTGAAAA CCAATCTCCA 301 CTTTCAGACG GCATTGTTTA G This corresponds to the amino acid sequence <SEQ ID 1644; ORF 558.ng>: g558.pep.. 1 MDACFFVIPA QAGIRRFGIV FKRSGRILAG AGMMPLYTFS ELYMLQQGTA 51 HQAPHCVLPE RGCPPIRFYR YKQTGFΝRKG MGIKSISDTS RAMPSEΝQSP 101 LSDGIV* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1645>: m558. seq.. 1 ATGAATGCTT GTTTTTTCGT CATTCCCACA CAGGCGGGAA TTCGGAGATT 51 CGGGATTGTT TTCAAACGTT CGGGTCGGAT TCTTGCCGGT GCAGGAATGA 101 TGCCCTTATA TACTTTCTCC GAGCTTTATA TGTTTCAACA GGGGACGGCA 151 CATCAAGCAC CGCACTGCGT GTTGCCCGAA CGAGACTACC CTCCGATTAG 201 ATTCTATCGC CATAAACAGA CGGGTTTCAA CCGAAAAGGA ATGGGGATAA 251 AGTCCATTTC CGACATCTsT CGGGCGATGC CGTCTGAAAA CCAATCTCCA 301 CTTTCAGACG GCATTGTTTA G This corresponds to the amino acid sequence <SEQ ID 1646; ORF 558>: m558 . pep . . 1 MNACFFVIPT QAGIRRFGIV FKRSGRILAG AGMMPLYTFS ELYMFQQGTA 51 HQAPHCVLPE RDYPPIRFYR HKQTGFNRKG MGIKSISDIX RAMPSENQSP 101 LSDGIV* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 558 shows 92.5% identity over a 106 aa overlap with a predicted ORF (ORF 558.ng) from N gonorrhoeae: m558/g558 10 20 30 40 50 60 m558. pep MΝACFFVIPTQAGIRRFGIVFKRSGRILAGAGMMPLYTFSELYMFQQGTAHQAPHCVLPE MMMMMMMMMMMMMMMMMMMMMMMMMMMMM g558 MDACFFVIPAQAGIRRFGIVFKRSGRILAGAGMMPLYTFSELYMLQQGTAHQAPHCVLPE 10 20 30 40 50 60 70 80 90 100 m558.pep RDYPPIRFYRHKQTGFΝRKGMGIKSISDIXRAMPSEΝQSPLSDGIVX I II II II I: II II II II I II II II I I lllllllllllllllll g558 RGCPPIRFYRYKQTGFΝRKGMGIKSISDTSRAMPSEΝQSPLSDGIVX 70 80 90 100 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1647>: a558. seq 1 ATGAATGCTT GTTTTTTCGT CATTCCCACA CAGGCGGGAA TTCGGAGATT 51 CGGGATTGTT TTCAAACGTT CGGGTCGGAT TCTTGCCGGT GCGGGAATGA 101 TGCCCTTATA TATAGTGGAT TAAATTTAAA TCAGGACAAG GCGACGAAGC 151 CGCAGACAGT ACAAATAGTA CGGCAAGGCG AGGCAACGCC GTACTGGTTT 201 AAATTTAATC CACTATACTT TCTCCGAGCT TTATATGTTT CAACAGAGGA 251 CGGCACATCA AGCACCGCAC TGCGTGTTGC CCGAACGAGA CTGCCCTCCG 301 ATTAGATTCT ATCGCTATAA ACAGACGGGT TTCAACCGAA AAGGAATGGG 351 AATGAAGTCC GTTTCCGACA CCTCTCGGGC GATGCCGTCT GAAAACCAAT 401 CTCCACTTTC AGACGGCATT GTTTAG This corresponds to the amino acid sequence <SEQ ID 1648; ORF 558. a>: a558.pep 1 MNACFFVIPT QAGIRRFGIV FKRSGRILAG AGMMPLYIVD *I*IRTRRRS 51 RRQYK*YGKA RQRRTGLNLI HYTFSELYMF QQRTAHQAPH CVLPERDCPP 101 IRFYRYKQTG FNRKGMGMKS VSDTSRAMPS ENQSPLSDGI V* m558/a558 70.2% identity in 141 aa overlap 10 20 30 m558.pep MNACFFVIPTQAGIRRFGIVFKRSGRILAGAGMMPLY I I I I I I II II II II I I II II I II II II I I M M M I I a558 MNACFFVIPT Q AGIRRFGIVFKRSGRILAGAGMMPLYIVDXIXIRTRRRSRRQYKXYGKA 10 20 30 40 50 60 40 50 60 70 80 m558. pep TFSELYMFQQGTAHQAPHCVLPERDYPPIRFYRHKQTGFNRKGMGIKS M I I II II I I llllllllllllll II I II I l II II II II MM I a558 R Q RRTGLNLIHYTFSELYMF QQ RTAH Q APHCVLPERDCPPIRFYRYKQTGFNRKGMGMKS 70 80 90 100 110 120 90 100 m558.pep ISDIXRAMPSENQSPLSDGIVX l a558 VSDTSRAMPSENQSPLSDGIVX 130 140 The following partial DNA sequence was identified in N.gonorrhoeae <SEQ ID 1649>: g560. seq 1 atgctcatca tccgcaacct gatttactgg ctgatactct gttccagcct 51 gattttcctc tttcccttta tgctgctcgc ctcgcctttc cgggacgggg 101 cgcacaagat ggcgcgggtc tgggtcggca tcctcaactg gtcgctcaaa 151 cacatcgtcg ggctcaaata ccgcatcatc ggcgcggaac acattccgga 201 ccgcccctcc gtcatctgcg ccaaacacca aagcggctgg gaaacgctcg 251 cgctccaaga gatttttccg ccgcaggttt acgttgccaa gcgcgagttg 301 ttcaaaatcc cctttttcgg ctggggcttg aaactggtca aaaccatagg 351 catagaccgc aacaaccgcc gcgaagccaa cgaacagctc ataaaacagg 401 gtttggcgcg caaaaacgaa ggttattgga ttaccatttt ccccgaaggc 451 acgcgccttg cgcccggaaa acgcggcaaa tacaaactcg gcggcgcgcg 501 catggcgaaa atgtttgaga tggacatcgt ccccgtcgcc ctcaacagcg 551 gcgaattttg gccgaaaaat tcctttctga aatatccggg ggaaatcacc 601 gtcatcatct gtccgaccat cccgcacgca agcggcagcg aagccgaatt 651 gatggaaaaa tgcgaacacc tcattgaaac gcaacaaccg cttatttccg 701 gcgcaggccc gtttgccgcc gaaatgccgt ctgaaaccgc atga This corresponds to the amino acid sequence <SEQ ID 1650; ORF 560.ng>: g560.pep.. 1 MLIIRNLIYW LILCSSLIFL FPFMLLASPF RDGAHKMARV WVGILNWSLK 51 HIVGLKYRII GAEHIPDRPS VICAKHQSGW ETLALQEIFP PQVYVAKREL 101 FKIPFFGWGL KLVKTIGIDR NNRREANEQL IKQGLARKNE GYWITIFPEG 151 TRLAPGKRGK YKLGGARMAK MFEMDIVPVA LNSGEFWPKN SFLKYPGEIT 201 VIICPTIPHA SGSEAELMEK CEHLIETQQP LISGAGPFAA EMPSET* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 1651>: m560. seq 1 ATGCTCATCA TCCGCAACCT GATTTACTGG CTGATACTCT GTTCCACCCT 51 GATTTTCCTC TTTCCCTTTA TGCTGCTCGC CTCGCCTTTC CGGGACGGGG 101 CGCACAAGAT GGCGCGGGTC TGGGTCGGCA TTCTCAACTG GTCGCTCAAA 151 CACATCGTCG GGCTCAAATA CCGCATCATC GGCGCGGAAA ACATCCCCGA 201 CCGCCCCGCC GTCATCTGCG CCAAACACCA AAGCGGCTGG GAAACGCTCG 251 CCCTTCAGGA CATTTTTCCG CCGCAGGTTT ACGTTGCCAA ACGCGAGTTG 301 TTCAAAATCC CCTTTTTCGG CTGGGGCTTG AAACTGGTCA AAACCATAGG 351 CATAGACCGC AACAACCGCC GCGAAGCCAA CGAGCAGCTC ATAAAACAGG 401 GGTTGGTGCG CAAAAACGAA GGCTATTGGA TTACCATTTT CCCCGAAGGC 451 ACGCGCCTTG CGCCCGGAAA ACGCGGCAAA TACAAACTCG GCGGCGCGCG 501 CATGGCGAAA ATGTTTGAGA TGGACATCGT CCCCGTCGCC CTCAACAGCG 551 GCGAATTTTG GCCGAAAAAC TCCTTTCTGA AATATCCGGG GGAAATCACC 601 GTCGTCATCT GTCCGACCAT CCCGCACGCA AGCGGCAGCG AAGCCGAATT 651 GATGGAAAAA TGCGAACATC TCATCGAAAC GCAACAACCG CTTATTTCCG 701 GCGCAGGCCC GTTTGCCGCC AAAATGCCGT CTGAAACCGC ATGA This corresponds to the amino acid sequence <SEQ ID 1652; ORF 560>: m560.pep 1 MLIIRNLIYW LILCSTLIFL FPFMLLASPF RDGAHKMARV WVGILNWSLK 51 HIVGLKYRII GAENIPDRPA VICAKHQSGW ETLALQDIFP PQVYVAKREL 101 FKIPFFGWGL KLVKTIGIDR NNRREANEQL IKQGLVRKNE GYWITIFPEG 151 TRLAPGKRGK YKLGGARMAK MFEMDIVPVA LNSGEFWPKN SFLKYPGEIT 201 WICPTIPHA SGSEAELMEK CEHLIETQQP LISGAGPFAA KMPSETA* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 560 shows 97.2% identity over a 246 aa overlap with a predicted ORF (ORF 560.ng) from N gonorrhoeae: m560/g560 10 20 30 40 50 60 m560.pep MLIIRΝLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVGILΝWSLKHIVGLKYRII 1 1 1 1 1 1 1 1 1 1 M 1 1 h l l M l M M 1 1 1 1 1 1 1 M 1 1 1 I I I I 1 1 1 I I I M 1 1 I I 1 1 1 I I 1 1 g560 MLIIRΝLIYWLILCSSLIFLFPFMLLASPFRDGAHKMARVWVGILΝWSLKHIVGLKYRII 10 20 30 40 50 60 70 80 90 100 110 120 m560. pep GAEΝIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFKIPFFGWGLKLVKTIGIDR I I hll llhll II II Ml III I llhll II II III III 11 III II II g560 GAEHIPDRPSVICAKHQSGWETLALQEIFPPQVYVAKRELFKIPFFGWGLKLVKTIGIDR 70 80 90 100 110 120 130 140 150 160 170 180 m560.pep NNRREANEQLIKQGLVRKNEGYWITIFPEGTRLAPGKRGKYKLGGARMAKMFEMDIVPVA g560 NNRREANEQLIKQGLARKNEGYWITI FPEGTRLAPGKRGKYKLGGARMAKMFEMDIVPVA 130 140 150 160 170 180 190 200 210 220 230 240 m560 . pep LNSGEFWPKNSFLKYPGEITWICPTIPHASGSEAELMEKCEHLIETQQPLISGAGPFAA MIMMMMMMMMIhlMMMMMMMMMMMMMMIMMMM g560 LNSGEFWPKNSFLKYPGEITVIICPTIPHASGSEAELMEKCEHLIETQQPLISGAGPFAA 190 200 210 220 230 240 m560.pep KMPSETAX MMM g560 EMPSETX The following partial DNA sequence was identified in N meningitidis <SEQ ID 1653>: a560. seq 1 ATGCTCATCA TCCGCAACCT GATTTACTGG CTGATACTCT GTTCCACCCT 51 GATTTTCCTC TTTCCCTTTA TGCTGCTCGC CTCGCCTTTC CGAGACGGGG 101 CGCACAAGAT GGCGCGGGTC TGGGTCAAAA TCCTCAACCT CTCGCTCAAA 151 CACATCGTCG GGCTCAAATA CCGCATCATC GGCGCGGAAA ACATCCCCGA 201 CCGCCCCGCC GTCATCTGCG CCAAACACCA AAGCGGCTGG GAAACGCTCG 251 CCCTTCAGGA CATTTTTCCG CCGCAGGTTT ACGTTGCCAA ACGCGAGTTG 301 TTCAAAATCC CCTTTTTCGG CTGGGGCTTG AAACTGGTCA AAACCATAGG 351 CATAGACCGC AACAACCGCC GCGAAGCCAA CGAGCAGCTC ATAAAACAGG 401 GGTTGGCGCG CAAAAACGAA GGCTATTGGA TTACCATTTT CCCCGAAGGC 451 ACACGCCTTG CGCCCGGAAA ACGCGGCAAA TACAAACTCG GCGGCGCGCG 501 CATGGCGAAA ATGTTTGAGA TGGACATCGT CCCCGTCGCC CTCAACAGCG 551 GCGAATTTTG GCCGAAAAAC TCCTTTCTGA AATATCCGGG GGAAATCACC 601 GTCGTCATCT GTCCGACCAT CCCGCACGCA AGCGGCAGCG AAGCCGAATT 651 GATGGGAAAA TGCGAACACC TCATCGAAAC GCAGCAGCCG CTCATTTCCG 701 GCGCAGGCCC GTTTGCCGCC AAAATGCCGT CTGAAACCGC ATGA This corresponds to the amino acid sequence <SEQ ID 1654; ORF 560.a>: a560.pep 1 MLIIRNLIYW LILCSTLIFL FPFMLLASPF RDGAHKMARV WVKILNLSLK 51 HIVGLKYRII GAENIPDRPA VICAKHQSGW ETLALQDIFP PQVYVAKREL 101 FKIPFFGWGL KLVKTIGIDR NNRREANEQL IKQGLARKNE GYWITIFPEG 151 TRLAPGKRGK YKLGGARMAK MFEMDIVPVA LNSGEFWPKN SFLKYPGEIT 201 WICPTIPHA SGSEAELMGK CEHLIETQQP LISGAGPFAA KMPSETA* m560/a560 98.4% identity in 247 aa overlap 10 20 30 40 50 60 m560.pep MLIIRNLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVGILNWSLKHIVGLKYRII II II I II II I II II II I II II II II II I II II I II II II II I III lllllllllllll a560 MLIIRNLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVKILNLSLKHIVGLKYRII 10 20 30 40 50 60 70 80 90 100 110 120 m560. pep GAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFKIPFFGWGLKLVKTIGIDR II II II II II I II II II II II I I II I I I II II I II II I I I II M II I I I II I M I II II I a560 GAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFKIPFFGWGLKLVKTIGIDR 70 80 90 100 110 120 130 140 150 160 170 180 m560. pep NNRREANEQLIKQGLVRKNEGYWITIFPEGTRLAPGKRGKYKLGGARMAKMFEMDIVPVA MMMMMMM MMMMMMMMMMMMMMMMMMMMMM a560 NNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGKYKLGGARMAKMFEMDIVPVA 130 140 150 160 170 180 190 200 210 220 230 240 m560.pep LNSGEFWPKNSFLKYPGEITWICPTIPHASGSEAELMEKCEHLIETQQPLISGAGPFAA II II II II II I I II I II II II II I II II I I I I I II II I I I I I II II II I I I I II II II I a560 LNSGEFWPKNSFLKYPGEITWICPTIPHASGSEAELMGKCEHLIETQQPLISGAGPFAA 190 200 210 220 230 240 m560.pep KMPSETAX a560 KMPSETAX The following partial DNA sequence was identified in N. meningitidis <SEQ ID 1655>: m561.seq. 1 ATGATACTGC CAGCCCGTTT TTCAGACGGC ATCAGCCTTT CCCTGCGCCT 51 GAAACTCCTG ACCGGACTGT GGGTCGGGTT GGCGGCATTG TCTGTCGTTT 101 TGACACTGCT GCTCTCTTTG CGTCTGGAAA ACGCGGCCTC CGTCATCGAA 151 GAGGCGGGCA ACTTGAGAAT GCAGGCATAC CGTCTGGCAT ACATGGCGGG 201 TGAAGGCTCG CCCCGTGCGC AAATTGACAA TCAGGTTGCC GAATTTGAAA 251 AAAGTTTAAA ACGCATTGCC CAAAGCGATG CCATCCATCC GCTGATTCCT 301 TCGGACACCC CTCTTGCTTA TGATTTGATA CAATCCATGC TGATTATAGA 351 TTGGCAGGCA CACATCCTCC CCCCGCTCCA GTCCTACCGG CGACCGACTC 401 AGGTCGATCT CTACCGCTTT GCCGGAAACA TCGAACTGTT TTTGCAGGCA 451 TTGGAAAATG CCAACGAAAA AAACACATGG TGGCTCAGGC GTTTTCAATG 501 GGCAATTATG TTGATGACGC TGGTGTCGTC TGTACTGATG CTGTTTTGGC 551 ACCAGATTTG GGTTATCCGG CCGCTGCAGG CGTTAAGGGA AGGTGCGGAA 601 CGCATCGGAC GGAGGTGTTT CGATATTCCG GTTCCCGAAG GCGGTACGCC 651 GGAATTCAAA CAGGTCGGGC GTTGTTTCAA TCAAATGGGC GGCAGGTTGA 701 AAATTTTATA TGATGATTTG GAAGGACAAG TCGCCGAGCA GACACGCAGT 751 CTCGAAAAAC AAAATCAAAA CCTGACCCTG CTGTACCAAA CTACACGGGA 801 CCTGCACCAA TCCTACATAC CGCAACAGGC TGCAGAACAT TTTCTAAACC 851 GTATCCTGCC CGCCGTAGGA GCAGATTCCG GCAGAGTTTG TTTGGACGGC 901 GGATCCGATG TTTATGTTTC CATTCATCAT GCGGATTGCG GCACAGCAGC 951 TTCGGATTTG GGGAAGTACC ATGAGGAAAT CTTCCCCATT GAGTACCAGA 1001 ACGAAACATT GGGCAGGCTG TTGCTCAGCT TTCCAAACGG CATTTCTCTT 1051 GATGAAGACG ACCGCATCCT GCTTCAAACA CTAGGCAGGC AATTGGGCGT 1101 ATCGCTTGCC GGCGCAAAAC AGGAGGAAGA AAAACGCCTG CTTGCAGTAT 1151 TGCAGGAACG CAACCTGATT GCGCAAGGAT TACATGACAG CATCGCACAA 1201 GCATTAACGT TCCTAAACCT ACAGGTACAG ATGCTGGAAA CCGCCTTTGC 1251 CGAAAACAAA CGGGAGGAAG CCGCAGAAAA CATCAGCTTT ATCAAAACAG 1301 GCGTGCAGGA ATGTTATGAA GATGTCCGCG AACTGCTGCT CAACTTCCGT 1351 ACCAAAATCA GCAATAAAGA ATTTCCCGAA GCCGTTGCCG ACCTATTCGC 1401 CCGCTTTACG CAACAAACCG GGATAACGGT CGAAACCGCC TGGGAAAACG 1451 GTTCGTTCCT GCCGCCTCAG GAAGCGCAGC TCCAAATGAT TTTTATCCTG 1501 CAGGAAAGCC TGTCCAACAT CCGCAAACAC GCCCGCGCCA CCCATGTAAA 1551 ATTCACCCTT TCCGAACACG GCGGACGCTT TACCATGACC ATCCAAGACA 1601 ACGGACAAGG TTTCGACACG GAGAAAATAG GAGAACCCAC GGGCAGCCAT 1651 GTCGGACTGC ACATCATGCA GGAGCGTGCC AAACGCATCC ATGCCGTTTT 1701 AGAAATCCGT TCCCAAGCTC AACAGGGAAC CACCGTCTCA TTGACGGTTG 1751 CATCTGAAGA AAGCTTGAAA TGA This corresponds to the amino acid sequence <SEQ ID 1656; ORF 561>: m561.pep 1 MILPARFSDG ISLSLRLKLL TGLWVGLAAL SWLTLLLSL RLEΝAASVIE 51 EAGΝLRMQAY RLAYMAGEGS PRAQIDΝQVA EFEKSLKRIA QSDAIHPLIP 101 SDTPLAYDLI QSMLIIDWQA HILPPLQSYR RPTQVDLYRF AGΝIELFLQA 151 LEΝAΝEKΝTW WLRRFQWAIM LMTLVSSVLM LFWHQIWVIR PLQALREGAE 201 RIGRRCFDIP VPEGGTPEFK QVGRCFΝQMG GRLKILYDDL EGQVAEQTRS 251 LEKQΝQΝLTL LYQTTRDLHQ SYIPQQAAEH FLΝRILPAVG ADSGRVCLDG 301 GSDVYVSIHH ADCGTAASDL GKYHEEIFPI EYQΝETLGRL LLSFPΝGISL 351 DEDDRILLQT LGRQLGVSLA GAKQEEEKRL LAVLQERΝLI AQGLHDSIAQ 401 ALTFLΝLQVQ MLETAFAEΝK REEAAEΝISF IKTGVQECYE DVRELLLΝFR 451 TKISΝKEFPE AVADLFARFT QQTGITVETA WEΝGSFLPPQ EAQLQMIFIL 501 QESLSNIRKH ARATHVKFTL SEHGGRFTMT IQDNGQGFDT EKIGEPTGSH 551 VGLHIMQERA KRIHAVLEIR SQAQQGTTVS LTVASEESLK * Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m561/g561 89.7% identity in 223 aa overlap 10 20 30 40 50 60 m561.pep MILPARFSDGISLSLRLKLLTGLWVGLAALSWLTLLLSLRLEΝAASVIEEAGΝLRMQAY I II I : II II I I II II II II I II II II II II II I I II II M I II II I II I II I I I : I II I g561 MILPTRFSDGIPLSLRLKLLTGLWVGLAALSWLTLLLSFRLEΝAASVIEEAGΝLKMQAY 10 20 30 40 50 60 70 80 90 100 110 120 m561.pep RLAYMAGEGSPRAQIDΝQVAEFEKSLKRIAQSDAIHPLIPSDTPLAYDLIQSMLIIDWQA II II II II I II II II II I M II II II I II M I II I II I II I I M II II II II II I I II I I g561 RLAYMAGEGSPRAQIDΝQIAEFEKSLKRISQSDAIHPLIPSDΝPLAYDLIQSMLIIDWQA 70 80 90 100 110 120 130 140 150 160 170 180 m561. pep HILPPLQSYRRPTQVDLYRFAGΝIELFLQALEΝAΝEKΝTWWLRRFQWAIMLMTLVSSVLM M II II I : II II II : : I I II II I II II I II II I I M II II II II II I M II I II I I I I I I g561 ΝILPPLQAYRRPTQIELYRFAGΝIELFLQALEΝAGEKΝTWWLRRFQWVIMLMTLVSSVLM 130 140 150 160 170 180 190 200 210 220 230 240 m561.pep LFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPEFKQVGRCFΝQMGGRLKILYDDL II II I II I II II II I I II II I II M lllllll M :: I g561 LFWHQIWVIRPLQALREGAERIGQRHFDIPVPEDVRPΝSΝRSGGVSTKWRSGX 190 200 210 220 230 250 260 270 280 290 300 m561.pep EGQVAEQTRSLEKQΝQΝLTLLYQTTRDLHQSYIPQQAAEHFLΝRILPAVGADSGRVCLDG The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1657>: a561. seq 1 ATGATACTGC CAGCCCGTTT TTCAGACGGC ATCAGCCTTT CCCTGCGCCT 51 GAAACTCCTG ACCGGACTGT GGGTCGGGTT GGCGGCATTG TCTGTCGTTT 101 TGACACTGCT GCTCTCTTTG CGTCTGGAAA ACGCGGCCTC CGTCATCGAA 151 GAGGCGGGCA ACTTGAGAAT GCAGGCATAC CGTCTGGCAT ACATGGCGGG 201 TGAAGGCTCG CCCCGTGCGC AAATTGACAA TCAGGTTGCC GAATTTGAAA 251 AAAGTTTAAA ACGCATTGCC CAAAGCGATG CCATCCATCC GCTGATTCCT 301 TCGGACACCC CTCTTGCTTA TGATTTGATA CAATCCATGC TGATTATAGA 351 TTGGCAGGCA CACATCCTCC CCCCGCTCCA GTCCTACCGG CGACCGACTC 401 AGGTCGATCT CTACCGCTTT GCCGGAAACA TCGAACTGTT TTTGCAGGCA 451 TTGGAAAATG CCAACGAAAA AAACACATGG TGGCTCAGGC GTTTTCAATG 501 GGCAATTATG TTGATGACGC TGGTGTCGTC TGTACTGATG CTGTTTTGGC 551 ACCAGATTTG GGTTATCCGG CCGCTGCAGG CGTTAAGGGA AGGTGCGGAA 601 CGCATCGGAC GGAGGTGTTT CGATATTCCG GTTCCCGAAG GCGGTACGCC 651 GGAATTCAAA CAGGTCGGGC GTTGTTTCAA TCAAATGGGC GGCAGGTTGA 701 AAATTTTATA TGATGATTTG GAAGGACAAG TCGCCGAGCA GACACGCAGT 751 CTCGAAAAAC AAAATCAAAA CCTGACCCTG CTGTACCAAA CTACACGGGA 801 TCTGCACCAA TCCTACATAC CGCAACAGGC TGCAGAACAT TTTCTAAACC 851 GTATCCTGCC CGCCGTAGGA GCAGATTCCG GCAGAGTTTG TTTGGACGGC 901 GGATCCGATG TTTATGTTTC CATTCATCAT GCGGATTGCG GCACAGCAGC 951 TTCGGATTTG GGGAAGTACC ATGAGGAAAT CTTCCCCATT GAGTACCAGA 1001 ACGAAACATT GGGCAGGCTG TTGCTCAGCT TTCCAAACGG CATTTCTCTT 1051 GATGAAGACG ACCGCATCCT GCTTCAAACA CTAGGCAGGC AATTGGGCGT 1101 ATCGCTTGCC GGCGCAAAAC AGGAGGAAGA AAAACGCCTG CTTGCAGTAT 1151 TGCAGGAACG CAACCTGATT GCGCAAGGAT TACATGACAG CATCGCACAA 1201 GCATTAACGT TCCTAAACCT ACAGGTACAG ATGCTGGAAA CCGCCTTTGC 1251 CGAAAACAAA CGGGAGGAAG CCGCAGAAAA CATCGGCTTC ATCAAAACAG 1301 GCGTGCAGGA ATGTTATGAA GATGTCCGCG AACTGCTGCT CAACTTCCGT 1351 ACCAAAATCA GTAATAAAGA ATTTCCCGAA GCCGTTGCCG ACCTATTCTC 1401 GCGCTTTACG CAACAGACCG GCACGACTGT CGAAACCGCT TGGGAAAACG 1451 GCACGCACCT GCCTACACAG GACGAGCAGC TCCAAATGAT TTTCATCCTG 1501 CAAGAAAGCT TGTCCAACAT CCGAAAACAT GCCCACGCCA CCCATATCAA 1551 ATTCAGACTG CTCAAACAGG ATGGAAGTTT TACAATGACC ATTCAAGACA 1601 ACGGACAGGG TTTTGACACG GAAAACATTG GAGAACCATC GGGCAGCCAT 1651 GTCGGACTGC ATATCATGCA GGAGCGTGCC AAACGCATCC ATGCCGTTTT 1701 AGAAATCCGT TCCCAAGCTC AACAGGGAAC CACCGTCTCA TTGACGGTTG 1751 CATCTGAAGA AAGCTTGAAA TGA This corresponds to the amino acid sequence <SEQ ID 1658; ORF 561. a>: a561 ■pep 1 MILPARFSDG ISLSLRLKLL TGLWVGLAAL SWLTLLLSL RLENAASVIE 51 EAGNLRMQAY RLAYMAGEGS PRAQIDNQVA EFEKSLKRIA QSDAIHPLIP 101 SDTPLAYDLI QSMLIIDWQA HILPPLQSYR RPTQVDLYRF AGNIELFLQA 151 LENANEKNTW WLRRFQWAIM LMTLVSSVLM LFWHQIWVIR PLQALREGAE 201 RIGRRCFDIP VPEGGTPEFK QVGRCFNQMG GRLKILYDDL EGQVAEQTRS 251 LEKQNQNLTL LYQTTRDLHQ SYIPQQAAEH FLNRILPAVG ADSGRVCLDG 301 GSDVYVSIHH ADCGTAASDL GKYHEEIFPI EYQNETLGRL LLSFPNGISL 351 DEDDRILLQT LGRQLGVSLA GAKQEEEKRL LAVLQERNLI AQGLHDSIAQ 401 ALTFLNLQVQ MLETAFAENK REEAAENIGF IKTGVQECYE DVRELLLNFR 451 TKISNKEFPE AVADLFSRFT QQTGTTVETA WENGTHLPTQ DEQLQMIFIL 501 QESLSNIRKH AHATHIKFRL LKQDGSFTMT IQDNGQGFDT ENIGEPSGSH 551 VGLHIMQERA KRIHAVLEIR SQAQQGTTVS LTVASEESLK m561/a561 96.9% identity in 590 aa overlap 10 20 30 40 50 60 m561 . pep MILPARFSDGISLSLRLKLLTGLWVGLAALSWLTLLLSLRLENAASVIEEAGNLRMQAY M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M a561 MILPARFSDGISLSLRLKLLTGLWVGLAALSWLTLLLSLRLENAASVIEEAGNLRMQAY 10 20 30 40 50 60 70 80 90 100 110 120 m561. pep RLAYMAGEGSPRAQIDNQVAEFEKSLKRIAQSDAIHPLIPSDTPLAYDLIQSMLIIDWQA II II II II II II II II I II II II II I II II I II I II II I II II II II I I II I II II I I I I a561 RLAYMAGEGSPRAQIDNQVAEFEKSLKRIAQSDAIHPLIPSDTPLAYDLIQSMLIIDWQA 70 80 90 100 110 120 130 140 150 160 170 180 m561.pep HILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTWWLRRFQWAIMLMTLVSSVLM II II II II II II II I II II I II I II I II II II I II I II II I I II I II I II II II II I II I a561 HILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTWWLRRFQWAIMLMTLVSSVLM 130 140 150 160 170 180 190 200 210 220 230 240 m561.pep LFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPEFKQVGRCFNQMGGRLKILYDDL II II II I II II II I II II I I I I I I II I II II II II I I II I II II I II I I II II I I I II I I a561 LFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPEFKQVGRCFNQMGGRLKILYDDL 190 200 210 220 230 240 250 260 270 280 290 300 m561. pep EGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQAAEHFLNRILPAVGADSGRVCLDG II II II I I II II II II II I II II I I I II II I I I I I I II I I I II II II I II II I II II M I a561 EGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQAAEHFLNRILPAVGADSGRVCLDG 250 260 270 280 290 300 310 320 330 340 350 360 m561.pep GSDVYVSIHHADCGTAASDLGKYHEEIFPIEYQNETLGRLLLSFPNGISLDEDDRILLQT I I II II II II II I I I II I II I II II II I II I I I II I I I I I I II II I I I I I II II II I I I I a561 GSDVYVSIHHADCGTAASDLGKYHEEIFPIEYQNETLGRLLLSFPNGISLDEDDRILLQT 310 320 330 340 350 360 370 380 390 400 410 420 m561. pep LGRQLGVSLAGAK Q EEEKRLLAVL Q ERNLIAQGLHDSIAQALTFLNLQVQMLETAFAENK MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a561 LGR Q LGVSLAGAK Q EEEKRLLAVL Q ERNLIAQGLHDSIAQALTFLNLQVQMLETAFAENK 370 380 390 400 410 420 430 440 450 460 470 480 m561.pep REEAAENISFIKTGVQECYEDVRELLLNFRTKISNKEFPEAVADLFARFTQQTGITVETA II I II II I : I II I II II II II II II I II II II II II II II I II II I M I I I I II II II I a561 REEAAENIGFIKTGVQECYEDVRELLLNFRTKISNKEFPEAVADLFSRFTQQTGTTVETA 430 440 450 460 470 480 490 500 510 520 530 540 m561.pep WENGSFLPPQEAQLQMIFILQESLSNIRKHARATHVKFTLSEHGGRFTMTIQDNGQGFDT llll: II M I II II II II II I I I I II I I : I I I : II I : : I llllllllllllll a 561 WENGTHLPTQDEQLQMIFILQESLSNIRKHAHATHIKFRLLKQDGSFTMTIQDNGQGFDT 490 500 510 520 530 540 550 560 570 580 590 m561.pep EKIGEPTGSHVGLHIMQERAKRIHAVLEIRSQAQQGTTVSLTVASEESLKX I : I II I M II I II II II II II II I II II II I II II II I II II II II I I I I I a561 ENIGEPSGSHVGLHIMQERAKRIHAVLEIRSQAQQGTTVSLTVASEESLKX 550 560 570 580 590 The following partial DNA sequence was ; identified in N gonorrh øeαe <SEQ ID 1659> g562. seq. 1 atggcaagcc cgtcgagtct gcctttcaat tcgggcaaga ccaaaccgac 51 ggcttttgcc gcgccggttt tggtcggaat catgttttcc acgccgctgc 101 gggcgcggcg caggtctttg tggcgcacgt cggtaacggt ttggtcgttg 151 gtcagtgcgt ggatggtggt cattgcgcct ttgacgatgc cgacgctttc 201 gctcaacact ttggcaaccg gcgagaggca gttggtggtg caggaagcgt 251 tggaaacgac ggtcatgtcg gcggtcagga cgctgtcgtt cacgccgtac 301 acgacggttg catcgacatc gtcgccgccc ggtgcggaaa tgaggacttt 351 tttcgcgccg ctttcgaggt ggattttggc tttttctttg ctggtgaacg 401 cgccggtgca ttccatgacc aaatcgacac cgagttcttt ccacggcagt 451 tcggcagggt tgcgggtcga gaagaagggg attttgtcgc cgttgacgat 501 gaggttgccg ccgtcgtggg atacgtcggc ttcaaagcgt ccgtgtacgg 551 tgtcgaattt ggtcagatgg gcgttggttt caaggctgcc gctggcgttg 601 acggcgacga tttggagttg gtcttga This corresponds to the amino acid sequence <SEQ ID 1660; ORF 562.ng>: g562.pep 1 MASPSSLPFΝ SGKTKPTAFA APVLVGIMFS TPLRARRRSL WRTSVTVWSL 51 VSAWMWIAP LTMPTLSLΝT LATGERQL V QEALETTVMS AVRTLSFTPY 101 TTVASTSSPP GAEMRTFFAP LSRWILAFSL LVΝAPVHSMT KSTPSSFHGS 151 SAGLRVEKKG ILSPLTMRLP PSWDTSASKR PCTVSΝLVRW ALVSRLPLAL 201 TATIWSWS* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1661>: m562.seq 1 ATGGCAAGCC CGTCGAGCCT GCCTTTCAAT TCGGGCAGTA CCAAACCGAC 51 GGCTTTTGCC GCGCCGGTTT TGGTCGGAAT CATGTTTTCC ACGCCGCTGC 101 GGGCGCGGCG CAGGTCTTTG TGGCGCACGT CGGTAACGGT TTGGTCGTTG 151 GTCAGCGCGT GGATGGTGGT CATCGCGCCT TTGACGATGC CGACGCTTTC 201 GCTCAACACT TTGGCAACCG GCGAGAGGCA GTTGGTGGTG CAGGAAGCGT 251 TGGAAACGAC GGTCATGTCG GCGGTCAGGA CGCTGTCGTT CACGCCGTAC 301 ACGACGGTTG CATCGACATC GTCGCCGCCC GGTGCGGAAA TGAGGACTTT 351 TTTCGCGCCG CTTTCGAGGT GGATTTTGGC TTTTTCTTTG CTGGTGAACG 401 CGCCGGTGCA TTCCATGACC AAATCGACAC CGAGTTCTTT CCACGGCAGT 451 TCGGCAGGGT TGCGGGTCGA GAAGAAGGGG ATTTTGTCGC CGTTGACGAT 501 GAGGTTGCCG CCGTCGTGGG ATACGTCGGC TTCAAAGCGT CCGTGCACGG 551 TGTCGAATTT GGTCAGATGG GCGTTGGTTT CAAGGCTGCC GCTGGCGTTG 601 ACGGCGACGA GTTGGAGTTG GTCTTGA This corresponds to the amino acid sequence <SEQ ID 1662; ORF 562>: m562.pep 1 MASPSSLPFΝ SGSTKPTAFA APVLVGIMFS TPLRARRRSL WRTSVTVWSL 51 VSAWMWIAP LTMPTLSLΝT LATGERQLVV QEALETTVMS AVRTLSFTPY 101 TTVASTSSPP GAEMRTFFAP LSRWILAFSL LVNAPVHSMT KSTPSSFHGS 151 SAGLRVEKKG ILSPLTMRLP PSWDTSASKR PCTVSNLVRW ALVSRLPLAL 201 TATSWSWS* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m562/g562 99.0% identity in 208 aa overlap 10 20 30 40 50 60 m562 . pep MASPSSLPFΝSGSTKPTAFAAPVLVGIMFSTPLRARRRSLWRTSVTVWSLVSAWMWIAP M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M g562 MASPSSLPFΝSGKTKPTAFAAPVLVGIMFSTPLRARRRSLWRTSVTVWSLVSAWMWIAP 10 20 30 40 50 60 70 80 90 100 110 120 m562.pep LTMPTLSLΝTLATGERQLWQEALETTVMSAVRTLSFTPYTTVASTSSPPGAEMRTFFAP II II II II II II I II II II I II I II II II I II II II II II II II II II II II II II I I II g562 LTMPTLSLΝTLATGERQLWQEALETTVMSAVRTLSFTPYTTVASTSSPPGAEMRTFFAP 70 80 90 100 110 120 130 140 150 160 170 180 m562.pep LSRWILAFSLLVΝAPVHSMTKSTPSSFHGSSAGLRVEKKGILSPLTMRLPPSWDTSASKR II II II II II II II II II II II I II II II II II II I II II I I I I II II I II II II I I M I g562 LSRWILAFSLLVΝAPVHSMTKSTPSSFHGSSAGLRVEKKGILSPLTMRLPPSWDTSASKR 130 140 150 160 170 180 190 200 209 m562.pep PCTVSΝLVRWALVSRLPLALTATSWSWSX II II II II II II II I II I II II I I II II g562 PCTVSΝLVRWALVSRLPLALTATIWSWSX The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 1663>: a562. seq 1 ATGGCAAGCC CGTCGAGTTT GTCTTTCAAT TCGGGCAGTA CCAAACCGAC 51 GGCTTTTGCC GCGCCAGTTT TGGTCGGAAT CATGTTTTCC ACGCCGCTGC 101 GGGCGCGGCG CAGGTCTTTG TGGCGCACGT CGGTAACGGT TTGGTCGTTG 151 GTCAGCGCGT GGATGGTGGT CATCGCGCCT TTGACGATGC CGACGCTTTC 201 GCTCAACACT TTGGCAACCG GCGAGAGGCA GTTGGTGGTG CAGGAAGCGT 251 TGGAAACGAC GGTCATGTCG GCGGTCAGGA TGCTGTCGTT CACGCCGTAC 301 ACGACGGTTG CATCGACATC GTCGCCGCCC GGTGCGGAAA TGAGGACTTT 351 TTTCGCGCCG CTTTCCAGAT GAACTTTGGC TTTTTCTTTG CTGGTGAACG 401 CGCCGGTGCA TTCCATGACC AAATCGACAC CGAGTTCTTT CCACGGCAGT 451 TCGGCAGGGT TGCGGGTCΝA GAAGAAΝGGG ATTTTGTCGC CGTTGACGAT 501 GAGGTTGCCG CCGTCGTGGG ATACGTCGGC TTCAAAGCGT CCGTGCACGG 551 TGTCGAATTT GGTGAGGTGG GCGTTGGTTT CAAGGCTGCC GCTGGCGTTG 601 ACGGCGACGA TTTGGAGTTG GTCTTGA This corresponds to the amino acid sequence <SEQ ID 1664; ORF 562.a>: a562.pep 1 MASPSSLSFΝ SGSTKPTAFA APVLVGIMFS TPLRARRRSL WRTSVTVWSL 51 VSAWMWIAP LTMPTLSLΝT LATGERQLVV QEALETTVMS AVRMLSFTPY 101 TTVASTSSPP GAEMRTFFAP LSR*TLAFSL LVNAPVHSMT KSTPSSFHGS 151 SAGLRVXKXG ILSPLTMRLP PSWDTSASKR PCTVSNLVRW ALVSRLPLAL 201 TATIWSWS* m562/a562 96.6% identity in 208 aa overlap 10 20 30 40 50 60 m562.pep MASPSSLPFNSGSTKPTAFAAPVLVGIMFSTPLRARRRSLWRTSVTVWSLVSAWMWIAP lllllll II II II II II II II I II I I I I II II II II I I I II I I I I M M M I I I M M I a562 MASPSSLSFNSGSTKPTAFAAPVLVGIMFSTPLRARRRSLWRTSVTVWSLVSAWMWIAP 10 20 30 40 50 60 70 80 90 100 110 120 m562. pep LTMPTLSLNTLATGERQLWQEALETTVMSAVRTLSFTPYTTVASTSSPPGAEMRTFFAP II II II II II II II II II II II II II II II I II II II II II I II II I I I II I I I I I I I I a562 LTMPTLSLNTLATGERQLWQEALETTVMSAVRMLSFTPYTTVASTSSPPGAEMRTFFAP 70 80 90 100 110 120 130 140 150 160 170 180 m562.pep LSRWILAFSLLVNAPVHSMTKSTPSSFHGSSAGLRVEKKGILSPLTMRLPPSWDTSASKR III II II II II II II II II II II II II II I II I I I II II I II I I I I I I II I I I I I I a562 LSRXTLAFSLLVNAPVHSMTKSTPSSFHGSSAGLRVXKXGILSPLTMRLPPSWDTSASKR 130 140 150 160 170 180 190 200 209 m562. pep PCTVSNLVRWALVSRLPLALTATSWSWSX II II I II II II II I II II II II I I I I I I a562 PCTVSNLVRWALVSRLPLALTATIWSWSX 190 200 The following partial DNA sequence was identified in N.gonorrhoeae <SEQ ID 1665>: g563.seq 1 ATGAACAAAA CCCTCTATCG TGTGATTTTC AACCGCAAAC GCGGTGCTGT 51 GGTAGCTGTT GCCGAAACCA CCAAGCGCGA AGGTAAAAGC TGTGCCGATA 101 GTGGTTCGGG CAGCGTTTAT GTGAAATCCG TTTCTTTCAT TCCTACTCAT 151 TCCAAAGCCT TTTGTTTTTC TGCATTAGGC TTTTCTTTAT GTTTGGCTTT 201 GGGTACGGTC AATATTGCTT TTGCTGACGG CATTATTACT GATAAAGCTG 251 CTCCTAAAAC CCAACAAGCC ACGATTCTGC AAACAGGTAA CGGCATACCG 301 CAAGTCAATA TTCAAACCcc tACTTCGGCa ggGGTTTCTG TTAATCAATA 351 TGCCCAGTTT GATGTGGGTA ATcgcGGGGC GATTTTAAAC AACAGTCGCA 401 GCAACACCCA AACACAGCTA GGCGGTTGGA TTCAAGGCAA TCCTTGGTTG 451 ACAAGGGGCG AAGCACGTGT GGTTGTAAAC CAAATCAACA GCAGCCATCC 501 TTCACAACTG AATGGCTATA TTGAAGTGGG TGGACGACGT GCAGAAGTCG 551 TTATTGCCAA TCCGGCAGGG ATTGCAGTCA ATGGTGGTGG TTTTATCAAT 601 GCTTCCCGTG CCACTTTGAC GACAGGCCAA CCGCAATATC AAGCAGGAGA 651 CTTTAGCGGC TTTAAGATAA GGCAAGGCAA TGCTGTAATC GCCGGACACG 701 GTTTGGATGC CCGTGATACC GATTTCACAC GTATTCTTTT GTATGCCAAC 751 AAAATCACCT TGATCAGTAC GGCCGAACAA GCAGGCATTC GTAATCAAGG 801 GCAGTTGTTT GCTTCTTCCG GTAATGTGGC GATTGATGCA AATGGCCGTT 851 TGGTCAATAG TGGCACGATG GCTGCCGCCA ATGTGCAAGA TATGAATAAT 901 ACAGCGGAAC ACAAAGTCAA TATCCGCAGT CAAGCCTTTG AAAACAGCGG 951 TACGGCGGTA TCGCAACAAG GCACTCAAAT TCACAGTCAA TCGATTCAAA 1001 ACACTGGCAA ATTATTGTCG GCAGGAACAG AGGATTTAGC CGTTTCAGGC 1051 AGCCTGAACA ATCAAAATGG CGAAATAGCG ACCAATCAAC AACTGATTAT 1101 TCACGATGGT CAGCAATCTA CCGTTGTCAT TGATAATACG AATGGCACGA 1151 TACAATCAGG CCGTGATGTT GCCATTCAGG CAAAATCGTT ATCCAACAAC 1201 GGCACACTTG CCGCTGATAA TAAACTGGAT ATTGCGTTAC AAGATGATTT 1251 TTATGTAGAA CGCAAGATCG TGGCGGGCAA TGAATTGTCG CTCAGTACAC 1301 GAGGCAGCCT GAAAAATTCA CATACCTTGC AAGCAGGAAA ACGCATTCGG 1351 ATTAAAGCAA ATAACCTTGA TAATGCAGTA CAAGGCAACA TTCAATCCGG 1401 CGGTACGACA GACATTGGCA CGCAGCACAA TTTAACCAAT AGAGGCTTGA 1451 TTGACGGACA ACAAACCAAA ATCCAAGCCG GGCAAATGAA TAATATCGGT 1501 ACAGGTCGGA TTTATGGCGA CAATATCGCT ATTGCGGCTA CCCGCTTAGA 1551 CAATCAAGAT GAAAACGGTA CAGGTGCCGC CATTGCGGCA CGCGAAAACC 1601 TGAATTTAGG CATTGAACAA TTAAATAACC GTGAAAACAG TCTGATTTAC 1651 AGCGGTAACG ATATGGCGGT TGGCGGCGCA TTAGATACCA ATGACCAAGC 1701 CACAGGCAAA GCCCAAAGGA TACACAATGC CGGCGCAATC ATTGAAGCTG 1751 CAGGCAAAAT GCGTTTAGGT GTAGAAAAGC TGCACAATAC CAATGAGCAT 1801 TTGAAAACGC AGTTGGTAGA AACAGGGCGC GAGCGTATTG TTGATTACGA 1851 AGCATTTGGA CGACACGAAT TATTGCGAGA AGGCACGCAA CATGAATTAG 1901 GCTGGTTTGT CTACAACAAT GAATCAGACC ACTTACGCAC CCCTGATGGA 1951 GTGGCGCATG AAAATTGGCA TAAATACGAT TATGAAAAAG TAACGCAAGA 2001 AACTCAAGTA ACCGGAACTG CGCCTGCTAA AATCATTGCA GGTAGCGATT 2051 TGATTATTGA TAGCAAAGCA GTCTTCAACA GCGACAGCCG AATCATTGCC 2101 GGCGGCCAAT TGCTTGTGCA AACAGAAAAA GACGGTTTGC ATAACGAGCA 2151 AACCTTTGGC GAGAAGAAAG TCTTCAGCGA AAATGGTAAG TTGCACAACT 2201 ACTGGCGTGC GCGTCGTAAA GGACATGATG AAACAGGGCA TCGTGAACAA 2251 AATTATACTT TGCCGGAGGA AATCACACGC GACATTTCAC TGGGTTCATT 2301 TGCCTATGAA TCGCATAGCA AAGCATTAAG CCGTCATGCG CCCAGCCAAG 2351 GCACTGAGTT GCCACAAAGT AACCGGGATA ATATCCGTAC TGCGAAAAGC 2401 AACGGTATTT CGCTACCCTA TACGCCCAAT TCTTTTACCC CATTACCCGG 2451 CAGCAGCTTA TACATTATCA ATCCTGCCAA TAAAGGCTAT CTTGTTGAAA 2501 CCGATCCACG CTTTGCCAAC TACCGTCAAT GGTTGGGTAG TGACTATATG 2551 CTGGGCAGCC TCAAACTAGA CCCAAACAAT TTACATAAAC GTTTGGGTGA 2601 TGGTTATTAC GAGCAACGTT TAATCAATGA ACAAATCGCA GAGCTGACAG 2651 GGCATCGTCG TTTAGACGGT TATCAAAACG ACGAAGAACA ATTTAAAGCC 2701 TTAATGGATA ATGGCGCGAC TGCGGCACGT TCGATGAATC TCAGCGTTGG 2751 CATTGCATTA AGTGCCGAGC AAGCAGCGCA ACTGACCAGC GATATTGTTT 2801 GGTTGGTACA AAAAGAAGTT AAACTTCCTG ATGGCGGCAC ACAAACCGTA 2851 TTGATGCCAC AGGTTTATGT ACGCGTTAAA AATGGCGGCA TAGACGGTAA 2901 AGGTGCATTG TTGTCAGGCA GCAATACACA AATCAATGTT TCAGGCAGCC 2951 TGAAAAACTC AGGCACGATT GCAGGGCGCA ATGCGCTTAT TATCAATACC 3001 GATACGCTAG ACAATATCGG TGGGCGTATT CATGCGCAAA AATCAGCGGT 3051 TACGGCCACA CAAGACATCA ATAATATTGG CGGCATTCTT TCTGCCGAAC 3101 AGACATTATT GCTCAATGCG GGTAACAACA TCAACAACCA AAGCACGGCC 3151 AAGAGCAGTC AAAATGCACA AGGTAGCAGC ACCTACCTAG ACCGAATGGC 3201 AGGTATTTAT ATCACAGGCA AAGAAAAAGG TGTTTTAGCA GCGCAGGCAG 3251 GCAAAGACAT CAACATCATT GCCGGTCAAA TCAGCAATCA ATCAGATCAA 3301 GGGCAAACCC GGCTGCAGGC AGGACGCGAC ATTAACCTGG ATACGGTACA 3351 AACCGGCAAA TATCAAGAAA TCCATTTTGA TGCCGATAAC CATACCATCC 3401 GAGGTTCAAC GAACGAAGTC GGCAGCAGCA TTCAAACAAA AGGCGATGTT 3451 ACCCtatTGT CAGGGAATAA TCTCAATGCC AAAGCTGCCG AAGTCGGCAG 3501 CGCAAAAGGC ACACTTGCCG TGTATGCTAA AAATGACATT ACTATCAGCT 3551 CAGGCATCCA TGCCGGCCAA GTTGATGATG CGTCCAAACA TACAGGCAGA 3601 AGCGGCGGCG GTAATAAATT AGTCATTACC GATAAAGCCC AAAGTCATCA 3651 CGAAACTGCT CAAAGCAGCA CCTTTGAAGG CAAGCAAGTT GTATTGCAGG 3701 CAGGAAACGA TGCCAACATC CTTGGCAGTA ATGTTATTTC CGATAATGGC 3751 ACCCGGATTC AAGCAGGCAA TCATGTTCGC ATTGGTACAA CCCAAACTCA 3801 AAGCCAAAGC GAAACCTATC ATCAAACCCA AAAATCAGGA TTGATGAGTG 3851 CAGGTATCGG CTTCACTATT GGCAGCAAGA CAAACACACA AGAAAACCAA 3901 TCCCAAAGCA ACGAACATAC AGGCAGTACC GTAGGCAGCC TGAAAGGCGA 3951 TACCACCATT GTTGCAAGCA AACACTACGA ACAAACCGGC AGCAACGTTT 4001 CCAGCCCTGA GGGCAACAAC CTTATCAGCA CGCAAAGTAT GGATATTGGC 4051 GCAGCACAAA ACCAATTAAA CAGCAAAACC ACCCAAACCT ACGAACAAAA 4101 AGGCTTAACG GTGGGCATTC AGTTCGCCCG TTACCGATTT GGCACAACAA 4151 GCGATTGCCG TAGCACACAA AGCAGCAAAC AAGTCGGACA AAGCAAAAAC 4201 GACCGCGTTA ATGCCATGGC GGCTGCCAAT GCAGGTTGGC AGGCCTATCA 4251 AACAGGCAAA GGCGCACAAA ACTTAGCCAA TGGTACAACC AATGCCAAAC 4301 AAGTCAGCAT CTCCATAACC TACGGCGAAC AGCAAAACCG ACAAACCACC 4351 CAAGTTCAAG CCAATCAAGC CCAAGCGAGT CAAATTCAAG CAGGCGGCAA 4401 AACTACCCTT TATTGCCGAA GGTGCGGCGA ACAATCCAAT ATCAACATCA 4451 CAGGCTCAGG TGTTTCAGGC AGAGCAGGAA CCGGCCTGAT TGCCGATAAG 4501 CAAATCCATC TGCAATCAGC CGAGCAAAGC AATACCGAAC GCAGCCAAAA 4551 CAAATCAGCA GGCTGGAACG CAGGTGCTGC CGTATCATTC GGACAAGGAG 4601 GCTGGTCATT AGGCGTTGCC GCAGGCGGCA ATGTCGGCAA AGGCTACGGC 4651 TATGGCGATA GCGTAACCCA CCGCCATAGC CATATTGGCG ACAAAGGCAG 4701 CCAAACCCTT ATCCAAAGTG GTGGCGATAC CATCATCAAA GGCGCGCAAG 4751 TACGCGGCAA AGGCGTACAA GTCAATGCCA AAAACCTAAG CATTCAAAGT 4801 GTACAAGATA GAGAAACTTA TCAAAGCAAA CAACAAAACG CCGGTGCACA 4851 AGTTACCGTA GGTTATGGCT TCAGTGCCAG TGGCGATTAC AGCCAAAGCA 4901 AAATCCGAGC CGACCATGCT TCGGTAACCG AGCAAAGCGG TATTTATGCC 4951 GGAGAAGACG GCTATCAAAT CAAGGTCGGA AACCATACAG GCCTCAAAGG 5001 CGGCATCATC ACCAGCAGCC AAAGCGCAAA AGACAAGGGT AAAAACCGAT 5051 TCAGCACAGG CACACTCGCC GGCAGTGATA TTCAAAATTA CAGCCAATAC 5101 GAAGGAAAAA GTTTTGGATT GGGTGCCAGC GTTGCCGTAA GCGGCAAAAC 5151 ACTGGGACAG GGCGCAAAAA ATAAACCTCA AGACAAACAC CTGACAAGCA 5201 TAGCCGATAA AAACGGCGCA AGTTCATCAG TAGGGTACGG CAGCGACAGC 5251 GACAGTCAAA GCAGCATCAC AAAAAGCGGC ATCAATACCC CCAAAAACAT 5301 TCAAATCACA GACGAAGCCG CACAAATCAG GCTGACAGGC AAAATAGCGG 5351 CACAAACCAA AGCCGATATT GATACAAACG TAACCACAGA CACCGCCGAA 5401 CGACATTCGG GCAGCCTGAA AAACATATTT GACAAAGATA GAGTGCAAAG 5451 TGAACTGGAT TTACAAAgaA CCGTCAGCCA AGATTTTAGT AAAAATGTTC 5501 AACAAACCAA TACCGAGATT AACCAACATT TAGACAAACT CAAAGCAGAC 5551 AAAGAAGCAG CCGAAACAGC AGCAGCCGAG GCATTAGCCA ATGGCGATAT 5601 GGAAACTGCC AAACGCAAAG CCCATGAAGC TCAAGATGCG GCAGCAAAAG 5651 CAGATAATTG GCAACAAGGC AAAGTCATTC TCAACATGTT AGCCTCAGGT 5701 TTAGCTGAGC CGACCCAAAG CGGAGCgggc ATCGCTGCGG CTACCGCATC 5751 GCCagaCGTA TCGTATGCGA TTGGACAGCA CTTTAAagaT TTAGCCGGTC 5801 AAAACGCGAA TGGCAAACTA ACCGCCAGTC AagaAACCGC TCACGTTCTT 5851 GCCCACGCGG TATTAGGAGC AGCGGTTGCC GCAGCATGAG GCAACAATGC 5901 CCCGGCAGGA GCATTGGGTG CGGGCGGGTc ggAagcggCC GCCCCAATCA 5951 TCGGCAAATG GCTGTACGGC AAAGGAGAcg gcggcagccT GAATgcggag 6001 gaaaAAGaga CCGTTTCGGC GATTACAAGG ATGCTGggta cGgctGCCGG 6051 AGCAGCTGAG GGAAACTCGT CCGCCGATGC TGTGTGGGGT TGTTTTcaaa 6101 cggctTCaga TTTCGCTTCC TCTTTTTCAT ATCCTATAAA CATGTGA This corresponds to the amino acid sequence <SEQ ID 1666; ORF 563.ng>: g563.pep.. 1 MNKTLYRVIF NRKRGAWAV AETTKREGKS CADSGSGSVY VKSVSFIPTH 51 SKAFCFSALG FSLCLALGTV NIAFADGIIT DKAAPKTQQA TILQTGNGIP 101 QVNIQTPTSA GVSVNQYAQF DVGNRGAILN NSRSNTQTQL GGWIQGNPWL 151 TRGEARVWN QINSSHPSQL NGYIEVGGRR AEWIANPAG IAVNGGGFIN 201 ASRATLTTGQ PQYQAGDFSG FKIRQGNAVI AGHGLDARDT DFTRILLYAN 251 KITLISTAEQ AGIRNQGQLF ASSGNVAIDA NGRLVNSGTM AAANVQDMNN 301 TAEHKVNIRS QAFENSGTAV SQQGTQIHSQ SIQNTGKLLS AGTEDLAVSG 351 SLNNQNGEIA TNQQLIIHDG QQSTWIDNT NGTIQSGRDV AIQAKSLSNN 401 GTLAADNKLD IALQDDFYVE RKIVAGNELS LSTRGSLKNS HTLQAGKRIR 451 IKANNLDNAV QGNIQSGGTT DIGTQHNLTN RGLIDGQQTK IQAGQMNNIG 501 TGRIYGDNIA IAATRLDNQD ENGTGAAIAA RENLNLGIEQ LNNRENSLIY 551 SGNDMAVGGA LDTNDQATGK AQRIHNAGAI IEAAGKMRLG VEKLHNTNEH 601 LKTQLVETGR ERIVDYEAFG RHELLREGTQ HELGWFVYNN ESDHLRTPDG 651 VAHENWHKYD YEKVTQETQV TGTAPAKIIA GSDLIIDSKA VFNSDSRIIA 701 GGQLLVQTEK DGLHNEQTFG EKKVFSENGK LHNYWRARRK GHDETGHREQ 751 NYTLPEEITR DISLGSFAYE SHSKALSRHA PSQGTELPQS NRDNIRTAKS 801 NGISLPYTPN SFTPLPGSSL YIINPANKGY LVETDPRFAN YRQWLGSDYM 851 LGSLKLDPNN LHKRLGDGYY EQRLINEQIA ELTGHRRLDG YQNDEEQFKA 901 LMDNGATAAR SMNLSVGIAL SAEQAAQLTS DIVWLVQKEV KLPDGGTQTV 951 LMPQVYVRVK NGGIDGKGAL LSGSNTQINV SGSLKNSGTI AGRNALIINT 1001 DTLDNIGGRI HAQKSAVTAT QDINNIGGIL SAEQTLLLNA GNNINNQSTA 1051 KSSQNAQGSS TYLDRMAGIY ITGKEKGVLA AQAGKDINII AGQISNQSDQ 1101 GQTRLQAGRD INLDTVQTGK YQEIHFDADN HTIRGSTNEV GSSIQTKGDV 1151 TLLSGNNLNA KAAEVGSAKG TLAVYAKNDI TISSGIHAGQ VDDASKHTGR 1201 SGGGNKLVIT DKAQSHHETA QSSTFEGKQV VLQAGNDANI LGSNVISDNG 1251 TRIQAGNHVR IGTTQTQSQS ETYHQTQKSG LMSAGIGFTI GSKTNTQENQ 1301 SQSNEHTGST VGSLKGDTTI VASKHYEQTG SNVSSPEGNN LISTQSMDIG 1351 AAQNQLNSKT TQTYEQKGLT VGIQFARYRF GTTSDCRSTQ SSKQVGQSKN 1401 DRVNAMAAAN AGWQAYQTGK GAQNLANGTT NAKQVSISIT YGEQQNRQTT 1451 QVQANQAQAΞ QIQAGGKTTL YCRRCGEQSN INITGSGVSG RAGTGLIADK 1501 QIHLQSAEQS NTERSQNKSA GWNAGAAVSF GQGGWSLGVA AGGNVGKGYG 1551 YGDSVTHRHS HIGDKGSQTL IQSGGDTIIK GAQVRGKGVQ VNAKNLSIQS 1601 VQDRETYQSK QQNAGAQVTV GYGFSASGDY SQSKIRADHA SVTEQSGIYA 1651 GEDGYQIKVG NHTGLKGGII TSSQSAKDKG KNRFSTGTLA GSDIQNYSQY 1701 EGKSFGLGAS VAVSGKTLGQ GAKNKPQDKH LTSIADKNGA SSSVGYGSDS 1751 DSQSSITKSG INTPKNIQIT DEAAQIRLTG KIAAQTKADI DTNVTTDTAE 1801 RHSGSLKNIF DKDRVQSELD LQRTVSQDFS KNVQQTNTEI NQHLDKLKAD 1851 KEAAETAAAE ALANGDMETA KRKAHEAQDA AAKADNWQQG KVILNMLASG 1901 LAEPTQSGAG IAAATASPDV SYAIGQHFKD LAGQNANGKL TASQETAHVL 1951 AHAVLGAAVA AAXGNNAPAG ALGAGGSEAA APIIGKWLYG KGDGGSLNAE 2001 EKETVSAITR MLGTAAGAAE GNSSADAVWG CFQTASDFAS SFSYPINM* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 1667>: m563. seq.. 1 ATGAATAAAA CTCTCTATCG TGTAATTTTC AACCGCAAAC GTGGGGCTGT 51 GGTAGCCGTT GCTGAAACTA CCAAGCGCGA AGGTAAAAGC TGTGCCGATA 101 GTGATTCAGG CAGCGCTCAT GTGAAATCTG TTCCTTTTGG TACTACTCAT 151 GCACCTGTTT GTCGTTCAAA TATCTTTTCT TTTTCTTTAT TGGGCTTTTC 201 TTTATGTTTG GCTGTAGGTA CGGCCAATAT TGCTTTTGCT GATGGCATTA 251 TTGCTGATAA AGCTGCTCCT AAAACTCAAC AAGCCACGAT TCTGCAAACA 301 GGTAACGGCA TACCGCAAGT CAATATTCAA ACCCCTACTT CGGCAGGGGT 351 TTCTGTTAAT CAATACGCCC AGTTTGATGT GGGTAATCGC GGGGCGATTT 401 TAAACAACAG CCGCAGCAAC ACCCAAACAC AGCTAGGCGG TTGGATTCAA 451 GGTAATCCTT GGTTGGCAAG GGGCGAAGCA CGTGTGGTTG TAAACCAAAT 501 CAACAGCAGC CATTCTTCAC AAATGAATGG CTATATTGAA GTGGGCGGAC 551 GACGTGCAGA AGTCGTTATT GCCAATCCGG CAGGGATTGC AGTCAATGGT 601 GGTGGTTTTA TCAATGCTTC CCGTGCCACT TTGACGACAG GCCAACCGCA 651 ATATCAAGCA GGAGACCTTA GCGGCTTTAA GATAAGGCAA GGCAATGTTG 701 TAATCGCCGG ACACGGTTTG GATGCCCGTG ATACCGATTT CACACGTATT 751 CTCAGTTATC ATTCCAAAAT TGATGCACCC GTATGGGGAC AAGATGTTCG 801 TGTCGTCGCG GGACAAAACG ATGTGGTCGC AACAGGTAAT GCACATTCGC 851 CTATTCTCAA TAATGCTGCT GCCAATACGT CAAACAATAC AGCCAACAAC 901 GGCACACATA TCCCTTTATT TGCGATTGAT ACAGGCAAAT TAGGAGGTAT 951 GTATGCCAAC AAAATCACCT TGATCAGTAC GGCCGAGCAA GCAGGCATTC 1001 GTAATCAAGG GCAGTTGTTT GCTTCTTCCG GTAATGTGGC GATTGATGCA 1051 AATGGCCGTT TAGTCAATAG TGGCACGATG GCTGCCGCCA ATGCGAAAGA 1101 TACGGATAAT ACAGCGGAAC ACAAAGTCAA TATCCGCAGT CAGGGCGTTG 1151 AAAACAGCGG TACGGCGGTA TCGCAACAAG GCACTCAAAT TCACAGTCAG 1201 TCGATTCAAA ACACTGGCAC ATTATTGTCC TCAGGCGAAA TATTGATTCA 1251 CAATTCGGGC AGCCTGAAAA ATGAAACATC AGGCACCATT GAAGCCGCTC 1301 GTTTGGCTAT TGATACCGAC ACACTTAATA ATCAAGGCAA ACTCTCTCAA 1351 ACAGGTTCAC AAAAACTCCA TATTGATGCA CAAGGCAAAA TGGATAACCG 1401 TGGCCGCATG GGTTTACAAG ATACCGCACC AACCGCGTCA AATGGTTCAA 1451 GCAATCAAAC CGGCAATAGT TACAATGCAT CTTTCCATTC ATCCACTACC 1501 ACACCAACAA CGGCAACAGG TACGGGTACT GCAACCGTTT CTATATCAAA 1551 CATAACTGCG CCTACCTTTG CTGATGGGAC AATTCGCACT CATGGTGCAC 1601 TGGATAATTC AGGCAGTATT ATTGCCAATG GTCAAACAGA TGTTAGTGCG 1651 CAACAAGGTT TAAATAATGC AGGACAAATA GACATTCATC AGTTAAATGC 1701 AAAAGGTTCG GCGTTTGACA ATCACAATGG AACAATTATC AGTGATGCGG 1751 TCCACATTCA AGCCGGCAGC CTGAATAATC AAAATGGCAA CATCACAACA 1801 CGCCAACAGT TAGAGATTGA AACCGATCAA CTGGATAACG CTCATGGCAA 1851 GTTATTATCA GCAGAAATAG CGGATTTAGC CGTTTCAGGC AGCCTGAACA 1901 ATCAAAATGG CGAAATAGCG ACCAATCAAC AACTGATTAT TCACGATGGT 1951 CAGCAATCTA CCGCTGTCAT TGATAATACG AATGGCACGA TACAATCAGG 2001 CCGTGATGTT GCTATTCAGG CAAAATCGTT ATCCAACAAC GGCACACTTG 2051 CCGCTGATAA TAAACTGGAT ATTGCGTTAC AAGATGATTT TTATGTAGAA 2101 CGCAATATCG TGGCGGGCAA TGAATTGTCG CTCAGTACAC GAGGCAGCCT 2151 GAAAAATTCA CATACTTTGC AAGCAGGAAA ACGCATTCGG ATTAAAGCAA 2201 ATAACCTTGA TAATGCAGCA CAAGGCAACA TTCAATCCGG CGGTACGACA 2251 GACATTGGCA CGCAGCACAA TTTAACCAAT AGAGGCTTGA TTGACGGACA 2301 ACAAACCAAA ATCCAAGCCG GGCAAATGAA TAATATCGGT ACAGGTCGGA 2351 TTTATGGCGA CAATATCGCT ATTGCGGCTA CCCGCTTAGA CAATCAAGAT 2401 GAAAACGGTA CAGGTGCCGC CATTGCGGCA CGTGAAAACC TGAATTTAGG 2451 CATCGGACAA TTAAACAACC GTGAAAACAG TCTGATTTAC AGCGGTAACG 2501 ATATGGCGGT TGGCGGCGCA TTAGATACCA ATGGCCAAGC CACAGGCAAA 2551 GCCCAAAGGA TACACAATGC CGGCGCAACC ATTGAAGCTG CAGGCAAAAT 2601 GCGTTTAGGT GTAGAAAAGC TGCACAATAC CAATGAGCAT TTGAAAACGC 2651 AGTTGGTAGA AACAGGGCGC GAGCATATTG TTGATTACGA AGCATTTGGA 2701 CGACACGAAT TATTGCGAGA AGGCACGCAA CATGAATTAG GCTGGTCTGT 2751 CTATAACGAT GAATCAGACC ACTTACGCAC CCCTGATGGA GCGGCGCATG 2801 AAAATTGGCA TAAATACGAT TATGAAAAAG TCACCCAAAA AACCCAAGTT 2851 ACCCAAACTG CGCCAGCCAA AATCATTTCA GGTAATGATT TAACCATTGA 2901 TGGTAAAGAA GTATTTAATA CCGATAGCCA AATCATTGCT GGTGGCAATC 2951 TCATTGTACA AACAGAAAAA GACGGTTTGC ATAACGAGCA AACCTTTGGC 3001 GAAAAGAAAG TATTCAGTGA AAATGGCAAA TTACACAGCT ATTGGCGTGA 3051 GAAACATAAA GGACGAGACT CAACGGGACA TAGCGAACAA AATTACACTT 3101 TGCCGGAGGA AATCACACGC AACATTTCAC TGGGTTCATT TGCCTATGAA 3151 TCGCATCGCA AAGCATTAAG CCATCATGCG CCCAGCCAAG GCACTGAGTT 3201 GCCGCAAAGC AACGGTATTT CGCTACCCTA TACGTCCAAT TCTTTTACCC 3251 CATTACCCAG CAGCAGCTTA TACATTATCA ATCCTGTCAA TAAAGGCTAT 3301 CTTGTTGAAA CCGATCCACG CTTTGCCAAC TACCGTCAAT GGTTGGGTAG 3351 TGACTATATG CTGGACAGCC TCAAACTAGA CCCAAACAAT TTACATAAAC 3401 GTTTGGGTGA TGGTTATTAC GAGCAACGTT TAATCAATGA ACAAATCGCA 3451 GAGCTGACAG GGCATCGTCG TTTAGACGGT TATCAAAACG ACGAAGAACA 3501 ATTTAAAGCC TTAATGGATA ATGGCGCGAC TGCGGCACGT TCGATGAATC 3551 TCAGCGTTGG CATTGCATTA AGTGCCGAGC AAGTAGCGCA ACTGACCAGC 3601 GATATTGTTT GGTTGGTACA AAAAGAAGTT AAGCTTCCTG ATGGCGGCAC 3651 ACAAACCGTA TTGGTGCCAC AGGTTTATGT ACGCGTTAAA AATGGCGACA 3701 TAGACGGTAA AGGTGCATTG TTGTCAGGCA GCAATACACA AATCAATGTT 3751 TCAGGCAGCC TGAAAAACTC AGGCACGATT GCAGGGCGCA ATGCGCTTAT 3801 TATCAATACC GATACGCTAG ACAATATCGG TGGGCGTATT CATGCGCAAA 3851 AATCAGCGGT TACGGCCACA CAAGACATCA ATAATATTGG CGGCATGCTT 3901 TCTGCCGAAC AGACATTATT GCTCAACGCA GGCAACAACA TCAACAGCCA 3951 AAGCACCACC GCCAGCAGTC AAAATACACA AGGCAGCAGC ACCTACCTAG 4001 ACCGAATGGC AGGTATTTAT ATCACAGGCA AAGAAAAAGG TGTTTTAGCA 4051 GCGCAGGCAG GAAAAGACAT CAACATCATT GCCGGTCAAA TCAGCAATCA 4101 ATCAGAGCAA GGGCAAACCC GGCTGCAAGC AGGGCGCGAC ATTAACCTAG 4151 ATACGGTACA AACCAGCAAA CATCAAGCAA CCCATTTTGA TGCCGATAAC 4201 CATGTTATTC GCGGTTCAAC GAACGAAGTC GGCAGCAGCA TTCAAACAAA 4251 AGGCGATGTT ACCCTATTGT CAGGGAATAA CCTCAATGCC AAAGCTGCCG 4301 AAGTCAGCAG CGCAAACGGT ACACTCGCTG TGTCTGCCAA AAATGACATC 4351 AACATCAGCG CAGGCATCAA CACGACCCAT GTTGATGATG CGTCCAAACA 4401 CACAGGCAGA AGCGGTGGTG GCAATAAATT AGTCATTACC GATAAAGCCC 4451 AAAGTCATCA CGAAACCGCC CAAAGCAGCA CCTTTGAAGG CAAGCAAGTT 4501 GTATTGCAGG CAGGAAACGA TGCCAACATC CTTGGCAGCA ATGTTATTTC 4551 CGATAATGGC ACCCAGATTC AAGCAGGCAA TCATGTTCGC ATTGGTACAA 4601 CCCAAACTCA AAGCCAAAGC GAAACCTATC ATCAAACCCA GAAATCAGGA 4651 TTGATGAGTG CAGGTATCGG CTTCACTATT GGCAGCAAGA CAAACACACA 4701 AGAAAACCAA TCCCAAAGCA ACGAACATAC AGGCAGTACC GTAGGCAGCT 4751 TGAAAGGCGA TACCACCATT GTTGCAGGCA AACACTACGA ACAAATCGGC 4801 AGTACCGTTT CCAGCCCGGA AGGCAACAAT ACCATCTATG CCCAAAGCAT 4851 AGACATTCAA GCGGCACACA ACAAATTAAA CAGTAATACC ACCCAAACCT 4901 ATGAACAAAA AGGCCTAACG GTGGCATTCA GTTCGCCCGT TACCGATTTG 4951 GCACAACAAG CGATTGCCGT AGCACAAAGC AGCAAACAAG TCGGACAAAG 5001 CAAAAACGAC CGCGTTAATG CCATGGCGGC TGCCAATGCA GGCTGGCAAG 5051 CCTATCAAAC AGGTAAGAGT GCACAAAACT TAGCCAATGG TACAACCAAT 5101 GCCAAACAAG TCAGCATCTC CATAACCTAC GGCGAACAGC AAAACCGACA 5151 AACCACCCAA GTTCAAGCCA ATCAAGCCCA AGCGAGTCAA ATTCAAGCAG 5201 GTGGTAAAAC CACATTAATC GCCACAGGCG CAGCAGAACA ATCCAATATC 5251 AACATCGCAG GCTCAGATGT TGCCGGCAAA GCAGGCACAA TCCTGATTGC 5301 CGATAACGAC ATCACACTCC AATCAGCCGA GCAAAGCAAT ACCGAACGCG 5351 GCCAAAACAA ATCGGCAGGC TGGAACGCAG GTGCTGCCGT ATCATTCGGA 5401 CAAGGAGGCT GGTCATTAGG CGTTACCGCA GGCGGCAATG TCGGCAAAGG 5451 CTACGGCAAT GGCGACAGCA TCACCCACCG CCATAGCCAT ATCGGCGACA 5501 AAGGCAGCCA AACCCTTATC CAAAGCGGTG GCGACACTAC CATCAAAGGC 5551 GCGCAAGTAC GCGGCAAAGG CGTACAAGTC AATGCCAAAA ACCTAAGTAT 5601 TCAAAGCGTA CAAGATAGAG AAACCTATCA AAGCAAACAA CAAAACGCCA 5651 GTGCACAAGT TACCGTAGGT TATGGCTTCA GTGCCGGTGG CGATTACAGC 5701 CAAAGCAAAA TCCGAGCCGA CCATGTTTCA GTAACCGAGC AAAGCGGTAT 5751 TTATGCCGGA GAAGACGGCT ATCAAATCAA GGTCGGAAAC CATACAGACC 5801 TCAAAGGCGG CATCATCACC AGTACCCAAA GCGCAGAAGA CAAGGGTAAA 5851 AACCGCTTTC AGACGGCCAC CCTCACCCAT AGCGACATCA AAAACCACAG 5901 CCAATACAAA GGCGAAAGTT TTGGATTGGG CGCAAGTGCG TCCATAAGCG 5951 GCAAAACACT GGGACAGGGC GCACAAAATA AACCTCAAAA CAAACACCTG 6001 ACAAGCGTAG CCGATAAAAA CAGCGCAAGT TCATCAGTGG GTTATGGCAG 6051 CGACAGCGAC AGTCAAAGCA GCATCACAAA AAGCGGCATC AACACCCGCA 6101 ACATTCAAAT CACCGACGAA GCCGCACAAA TCCGGCTGAC AGGCAAAACA 6151 GCGGCACAAA CCAAAGCCGA TATTGATACA AACGTAACCA CAGACACCGC 6201 CGAACGACAT TCGGGCAGCT TGAAGAACAC CTTCAACAAA GAAGCGGTGC 6251 AAAGTGAACT GGATTTACAA AGAACCGTCA GCCAAGATTT TAGTAAAAAT 6301 GTTCAACAAG CCAATACCGA GATTAACCAA CATTTAGACA AACTCAAAGC 6351 AGACAAAGAA GCAGCCGAAA CAGCAGCAGC CGAGGCATTA GCCAATGGCG 6401 ATATGGAAAC TGCCAAACGC AAAGCCCATG AAGCTCAAGA TGCGGCAGCA 6451 AAAGCAGATA ATTGGCAACA AGGCAAAGTC ATTCTCAACA TGTTAGCCTC 6501 AGGTTTAGCT GCGCCGACCC AAAGCGGAGC GGGCATCGCT GCGGCTACCG 6551 CATCGCCAGC CGTATCGTAT GCGATTGGAC AGCACTTTAA AGATTTAGCC 6601 GGTCAAAACG CGAATGGTAA ACTAACCGCC AGTCAAGAAA CCGCACACGT 6651 TCTTGCCCAC GCGGTATTAG GAGCAGCGGT TGCCGCAGTA GGAGACAACA 6701 ATGCTCTAGC AGGAGCATTG AGTGCGGGCG GGTCGGAAGC GGCTGCGCCT 6751 TACATCAGCA AATGGTTATA CGGCAAAGAA AAAGGAAGCG ACTTAACGGC 6801 GGAAGAGAAA GAGACTGTAA CAGCGATTAC AAATGTATTG GGTACGGCTA 6851 CGGGTGCGGC AGTCGGCAAC AGCGCAACAG ATGCAGCGCA AGGCAGCCTG 6901 AATGCGCAAA GTGCGGTGGA GAATAATGAT ACTGTAGAGC AAGTGAAATT 6951 TGCTCTTAGG CACCCTAGAA TTGCTATTGC AATTGGATCT GTACATAAAG 7001 ATCCTGGCTC TACATTAGAG CCTAATATTT CAACAATTGC TTCAACTTTT 7051 CAATTAAATT TATTTCCTAA TAGTGAATTT GGTGGTGAAG GTGGAGTTGG 7101 CAATGCATTC AGGCACGTTT TATGGCAAGC AACCATCACA CGAGAATTTG 7151 GCAAAGATAT TGCTGTTAAA GTAGGAAATA GTCATGAAAG TGGGGAAAAA 7201 ATTAATTATT CTATAAGACG TAATCTTTCA TTAGATAAAG CAGATGAAAT 7251 GATTGATCAA CTAAATAACG AAATAGGAAG AGAAATAGCA TTAAATACCA 7301 ATAGGTTAAA CACAAAAGAG TTAGTTGGAT TAATTCTGGA AACTTATAAA 7351 AATAATGGTT TTTATCAAGC AGAAAGAAAC AGTAATGGAA ATTATGATGT 7401 TGTAAGAAAA AGATTATCTG AAAAAGATTA CCAGAATACA AGCAATATAT 7451 TGATTCACTT AGATAATACT GGTGCCGGAT TTAAAATTCA GCAGAGGAGA 7501 AAACAAATCA GAGCACAAAT TTCAGCCAGA CAATGGAGAA GATAA This corresponds to the amino acid sequence <SEQ ID 1668; ORF 563>: m563.pep.. 1 MNKTLYRVIF NRKRGA AV AETTKREGKS CADSDSGSAH VKSVPFGTTH 51 APVCRSNIFS FSLLGFSLCL AVGTANIAFA DGIIADKAAP KTQQATILQT 101 GNGIPQVNIQ TPTSAGVSVN QYAQFDVGNR GAILNNSRSN TQTQLGGWIQ 151 GNPWLARGEA RVWNQINSS HSSQMNGYIE VGGRRAEWI ANPAGIAVNG 201 GGFINASRAT LTTGQPQYQA GDLSGFKIRQ GNWIAGHGL DARDTDFTRI 251 LSYHΞKIDAP VWGQDVRWA GQNDWATGN AHSPILNNAA ANTSNNTANN 301 GTHIPLFAID TGKLGGMYA KITLISTAEQ AGIRNQGQLF ASSGNVAIDA 351 NGRLVNSGTM AAANAKDTDN TAEHKVNIRS QGVENSGTAV SQQGTQIHSQ 401 SIQNTGTLLS SGEILIHNSG SLKNETSGTI EAARLAIDTD TLNNQGKLSQ 451 TGSQKLHIDA QGKMDNRGRM GLQDTAPTAS NGSSNQTGNS YNASFHSSTT 501 TPTTATGTGT ATVSISNITA PTFADGTIRT HGALDNSGSI IANGQTDVSA 551 QQGLNNAGQI DIHQLNAKGS AFDNHNGTII SDAVHIQAGS LNNQNGNITT 601 RQQLEIETDQ LDNAHGKLLS AEIADLAVSG SLNNQNGEIA TNQQLIIHDG 651 QQSTAVIDNT NGTIQSGRDV AIQAKSLSNN GTLAADNKLD IALQDDFYVE 701 RNIVAGNELS LSTRGSLKNS HTLQAGKRIR IKANNLDNAA QGNIQSGGTT 751 DIGTQHNLTN RGLIDGQQTK IQAGQMNNIG TGRIYGDNIA IAATRLDNQD 801 ENGTGAAIAA RENLNLGIGQ LNNRENSLIY SGNDMAVGGA LDTNGQATGK 851 AQRIHNAGAT IEAAGKMRLG VEKLHNTNEH LKTQLVETGR EHIVDYEAFG 901 RHELLREGTQ HELGWSVYND ESDHLRTPDG AAHENWHKYD YEKVTQKTQV 951 TQTAPAKIIS GNDLTIDGKE VFNTDSQIIA GGNLIVQTEK DGLHNEQTFG 1001 EKKVFSENGK LHSYWREKHK GRDSTGHSEQ NYTLPEEITR NISLGSFAYE 1051 SHRKALSHHA PSQGTELPQS NGISLPYTSN SFTPLPSSSL YIINPVNKGY 1101 LVETDPRFAN YRQWLGSDYM LDSLKLDPNN LHKRLGDGYY EQRLINEQIA 1151 ELTGHRRLDG YQNDEEQFKA LMDNGATAAR SMNLSVGIAL SAEQVAQLTΞ 1201 DIVWLVQKEV KLPDGGTQTV LVPQVYVRVK NGDIDGKGAL LSGSNTQINV 1251 SGSLKNSGTI AGRNALIINT DTLDNIGGRI HAQKSAVTAT QDINNIGGML 1301 SAEQTLLLNA GNNINSQSTT ASSQNTQGSS TYLDRMAGIY ITGKEKGVLA 1351 AQAGKDINII AGQISNQSEQ GQTRLQAGRD INLDTVQTSK HQATHFDADN 1401 HVIRGSTNEV GSSIQTKGDV TLLSGNNLNA KAAEVSSANG TLAVSAKNDI 1451 NISAGINTTH VDDASKHTGR SGGGNKLVIT DKAQSHHETA QSSTFEGKQV 1501 VLQAGNDANI LGSNVISDNG TQIQAGNHVR IGTTQTQSQS ETYHQTQKSG 1551 LMSAGIGFTI GSKTNTQENQ SQSNEHTGST VGSLKGDTTI VAGKHYEQIG 1601 STVSSPEGNN TIYAQSIDIQ AAHNKLNSNT TQTYEQKGLT VAFSSPVTDL 1651 AQQAIAVAQS SKQVGQSKND RVNAMAAANA GWQAYQTGKS AQNLANGTTN 1701 AKQVSISITY GEQQNRQTTQ VQANQAQASQ IQAGGKTTLI ATGAAEQSNI 1751 NIAGSDVAGK AGTILIADND ITLQSAEQSN TERGQNKSAG WNAGAAVSFG 1801 QGGWSLGVTA GGNVGKGYGN GDSITHRHSH IGDKGSQTLI QSGGDTTIKG 1851 AQVRGKGVQV NAKNLSIQSV QDRETYQSKQ QNASAQVTVG YGFSAGGDYS 1901 QSKIRADHVS VTEQSGIYAG EDGYQIKVGN HTDLKGGIIT STQSAEDKGK 1951 NRFQTATLTH SDIKNHSQYK GESFGLGASA SISGKTLGQG AQNKPQNKHL 2001 TSVADKNSAS SSVGYGSDSD SQSSITKSGI NTRNIQITDE AAQIRLTGKT 2051 AAQTKADIDT NVTTDTAERH SGSLKNTFNK EAVQSELDLQ RTVSQDFSKN 2101 VQQANTEINQ HLDKLKADKE AAETAAAEAL ANGDMETAKR KAHEAQDAAA 2151 KADNWQQGKV ILNMLASGLA APTQSGAGIA AATASPAVSY AIGQHFKDLA 2201 GQNANGKLTA SQETAHVLAH AVLGAAVAAV GDNNALAGAL SAGGSEAAAP 2251 YISKWLYGKE KGSDLTAEEK ETVTAITNVL GTATGAAVGN SATDAAQGSL 2301 NAQSAVENND TVEQVKFALR HPRIAIAIGS VHKDPGSTLE PNISTIASTF 2351 QLNLFPNSEF GGEGGVGNAF RHVLWQATIT REFGKDIAVK VGNSHESGEK 2401 INYSIRRNLS LDKADEMIDQ LNNEIGREIA LNTNRLNTKE LVGLILETYK 2451 NNGFYQAERN SNGNYDWRK RLSEKDYQNT SNILIHLDNT GAGFKIQQRR 2501 KQIRAQISAR QWRR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 563 shows 79.1% identity over a 2316 aa overlap with a predicted ORF (ORF 563.ng) from N gonorrhoeae: m563/g563 10 20 30 40 50 g563. pep MΝKTLYRVIFΝRKRGAWAVAETTKREGKSCADSGSGSVYVKSVSFIPTH SKAFC M l : M M | M M M M h M I M I I I h i m563 . pep MΝKTLYRVIFΝRKRGAWAVAETTKREGKSCADSDSGSAHVKSVPFGTTHAPVCRSΝIFS 10 20 30 40 50 60 60 70 80 90 100 110 g563.pep FSALGFSLCLALGTVΝIAFADGIITDKAAPKTQQATILQTGΝGIPQVΝIQTPTSAGVSVΝ I I m563.pep FSLLGFSLCLAVGTAΝIAFADGIIADKAAPKTQQATILQTGΝGIPQVΝIQTPTSAGVSVΝ 70 80 90 100 110 120 120 130 140 150 160 170 g563.pep QYAQFDVGΝRGAILΝΝSRSΝTQTQLGGWIQGΝPWLTRGEARVWΝQIΝSSHPSQLΝGYIE M : M M M M l M M I I M i l l M | I M M M I I M M I I m563 . pep QYAQFDVGΝRGAILΝΝSRSΝTQTQLGGWIQGΝPWLARGEARVWΝQIΝSSHSSQMΝGYIE 130 140 150 160 170 180 180 190 200 210 220 230 g563 . pep VGGRRAEWIAΝPAGIAVΝGGGFIΝASRATLTTGQPQYQAGDFSGFKIRQGΝAVIAGHGL E 1 1 1 1 1 1 E 1 1 1 E 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 ] 1 1 1 1 1 1 1 1 1 1 1 = I E 1 1 1 1 1 1 1 = L I E 1 1 1 1 m563 . pep VGGRRAEWIAΝPAGIAVΝGGGFIΝASRATLTTGQPQYQAGDLSGFKIRQGΝWIAGHGL 190 200 210 220 230 240 240 g563 . pep DARDTDFTRIL m563 . pep DARDTDFTRILSYHSKIDAPVWGQDVRWAGQΝDWATGΝAHSPILΝΝAAAΝTSΝΝTAΝΝ 250 260 270 280 290 300 250 260 270 280 290 g563 . pep LYAΝKITLISTAEQAGIRΝQGQLFASSGΝVAIDAΝGRLVΝSGTM MMMMMMMMMMMMMMMMMMMMMM m563.pep GTHIPLFAIDTGKLGGMYAΝKITLISTAEQAGIRΝQGQLFASSGΝVAIDAΝGRLVΝSGTM 310 320 330 340 350 360 300 310 320 330 340 g563.pep AAANVQDMNNTAEHKVNIRSQAFENSGTAVSQQGTQIHSQSIQNTGKLLSAGT- m563.pep AAANAKDTDNTAEHKVNIRSQGVENSGTAVSQQGTQIHSQSIQNTGTLLSSGEILIHNSG 370 380 390 400 410 420 g563.pep m563.pep SLKNETSGTIEAARLAIDTDTLNNQGKLSQTGSQKLHIDAQGKMDNRGRMGLQDTAPTAS 430 440 450 460 470 480 g563.pep m563. pep NGSSNQTGNSYNASFHSSTTTPTTATGTGTATVSISNITAPTFADGTIRTHGALDNSGSI 490 500 510 520 530 540 g563.pep m563.pep IANGQTDVSAQQGLNNAGQIDIHQLNAKGSAFDNHNGTIISDAVHIQAGSLNNQNGNITT 550 560 570 580 590 600 350 360 370 380 g563. pep EDLAVSGSLNNQNGEIATNQQLIIHDGQQSTWIDNT MMMMMMMMMMMMMMIhMIM m563.pep RQQLEIETDQLDNAHGKLLSAEIADLAVSGSLNNQNGEIATNQQLIIHDGQQSTAVIDNT 610 620 630 640 650 660 390 400 410 420 430 440 g563.pep NGTIQSGRDVAIQAKSLSNNGTLAADNKLDIALQDDFYVERKIVAGNELSLSTRGSLKNS m563.pep NGTIQSGRDVAIQAKSLSNNGTLAADNKLDIALQDDFYVERNIVAGNELSLSTRGSLKNS 670 680 690 700 710 720 450 460 470 480 490 500 g563.pep HTLQAGKRIRIKANNLDNAVQGNIQSGGTTDIGTQHNLTNRGLIDGQQTKIQAGQMNNIG m563.pep HTLQAGKRIRIKANNLDNAAQGNIQSGGTTDIGTQHNLTNRGLIDGQQTKIQAGQMNNIG 730 740 750 760 770 780 510 520 530 540 550 560 g563.pep TGRIYGDNIAIAATRLDNQDENGTGAAIAARENLNLGIEQLNNRENSLIYSGNDMAVGGA MMMMMMMMMMMMMMMMMMM MMIMMMMMMMM m563.pep TGRIYGDNIAIAATRLDNQDENGTGAAIAARENLNLGIGQLNNRENSLIYSGNDMAVGGA 790 800 810 820 830 840 570 580 590 600 610 620 g563. pep LDTNDQATGKAQRIHNAGAIIEAAGKMRLGVEKLHNTNEHLKTQLVETGRERIVDYEAFG MM MMMMMMM I II II II I II I II II I II II 11 II I II I II h 11 II II II m563.pep LDTNGQATGKAQRIHNAGATIEAAGKMRLGVEKLHNTNEHLKTQLVETGREHIVDYEAFG 850 860 870 880 890 900 630 640 650 660 670 680 g563. pep RHELLREGTQHELGWFVYNNESDHLRTPDGVAHENWHKYDYEKVTQETQVTGTAPAKIIA MMMMIMMM II h I II II II II h II I II II II II 111 h II II m563. pep RHELLREGTQHELGWSVYNDESDHLRTPDGAAHENWHKYDYEKVTQKTQVTQTAPAKIIS 910 920 930 940 950 960 690 700 710 720 730 740 g563.pep GSDLIIDSKAVFNSDSRIIAGGQLLVQTEKDGLHNEQTFGEKKVFSENGKLHNYWRARRK hll MM MhlhMMhhMMMMMMMMMIMMMIhMI - m563.pep GNDLTIDGKEVFNTDSQIIAGGNLIVQTEKDGLHNEQTFGEKKVFSENGKLHSYWREKHK 970 980 990 1000 1010 1020 750 760 770 780 790 800 g563. pep GHDETGHREQNYTLPEEITRDISLGSFAYESHSKALSRHAPSQGTELPQSNRDNIRTAKS hi Ml MMMMMMMMMMMM I Ii h II M 111 II II 11 m563. pep GRDSTGHSEQNYTLPEEITRNISLGSFAYESHRKALSHHAPSQGTELPQSN 1030 1040 1050 1060 1070 810 820 830 840 850 860 g563.pep NGISLPYTPNSFTPLPGSSLYIINPANKGYLVETDPRFANYRQWLGSDYMLGSLKLDPNN m563.pep -GISLPYTSNSFTPLPSSSLYIINPVNKGYLVETDPRFANYRQWLGSDYMLDSLKLDPNN 1080 1090 1100 1110 1120 1130 870 880 890 900 910 920 g563. pep LHKRLGDGYYEQRLINEQIAELTGHRRLDGYQNDEEQFKALMDNGATAARSMNLSVGIAL MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM m563. pep LHKRLGDGYYEQRLINEQIAELTGHRRLDGYQNDEEQFKALMDNGATAARSMNLSVGIAL 1140 1150 1160 1170 1180 1190 930 940 950 960 970 980 g563. pep SAEQAAQLTSDI WLVQKEVKLPDGGTQTVLMPQVYVRVKNGGIDGKGALLSGSNTQINV 111 I = 111111111111111111111111 E I = 1111111111 E 11 i 1111111111111 m563. pep SAEQVAQLTSDIVWLVQKEVKLPDGGTQTVLVPQVYVRVKNGDIDGKGALLSGSNTQINV 1200 1210 1220 1230 1240 1250 990 1000 1010 1020 1030 1040 g563.pep SGSLKNSGTIAGRNALIINTDTLDNIGGRIHAQKSAVTATQDINNIGGILSAEQTLLLNA Mi MMM Ml I MM I I Ml I I M ' ! MMM: ; M l m563.pep SGSLKNSGTIAGRNALIINTDTLDNIGGRIHAQKSAVTATQDINNIGGMLSAEQTLLLNA 1260 1270 1280 1290 1300 1310 1050 1060 1070 1080 1090 1100 g563. pep GNNINNQSTAKSSQNAQGSSTYLDRMAGIYITGKEKGVLAAQAGKDINIIAGQISNQSDQ llllhllh MlhlMMMMMMMMMMMMMMMMMIMMMM m563.pep GNNINSQSTTASSQNTQGSSTYLDRMAGIYITGKEKGVLAAQAGKDINIIAGQISNQSEQ 1320 1330 1340 1350 1360 1370 1110 1120 1130 1140 1150 1160 g563. pep GQTRLQAGRDINLDTVQTGKYQEIHFDADNHTIRGSTNEVGSSIQTKGDVTLLSGNNLNA MMMIMMMMMhhl II II II h II II II I II I II II II II I II II II II I m563. pep GQTRLQAGRDINLDTVQTSKHQATHFDADNHVIRGSTNEVGSSIQTKGDVTLLSGNNLNA 1380 1390 1400 1.410 1420 1430 1170 1180 1190 1200 1210 1220 g563.pep KAAEVGSAKGTLAVYAKNDITISSGIHAGQVDDASKHTGRSGGGNKLVITDKAQSHHETA llllhlhlllll llllhlhlh : MMMMMMMMIMMMMMMM m563. pep KAAEVSSANGTLAVSAKNDINI SAGINTTHVDDASKHTGRSGGGNKLVITDKAQSHHETA 1440 1450 1460 1470 1480 1490 1230 1240 1250 1260 1270 1280 g563. pep QSSTFEGKQWLQAGNDANILGSNVISDNGTRIQAGNHVRIGTTQTQSQSETYHQTQKSG m563.pep QSSTFEGKQWLQAGNDANILGSNVISDNGTQIQAGNHVRIGTTQTQSQSETYHQTQKSG 1500 1510 1520 1530 1540 1550 1290 1300 1310 1320 1330 1340 g563.pep LMSAGIGFTIGSKTNTQENQSQSNEHTGSTVGSLKGDTTIVASKHYEQTGSNVSSPEGNN m563.pep LMSAGIGFTIGSKTNTQENQSQSNEHTGSTVGSLKGDTTIVAGKHYEQIGSTVSSPEGNN 1560 1570 1580 1590 1600 1610 1350 1360 1370 1380 1390 1400 g563.pep LI:STQSMDIGAAQNQLNSKTTQTYEQKGLTVGIQFARYRFGTTSDCRSTQSSKQVGQSKN I Mhll IhlMlhlMMIMMIh : : - = MMIIMMM m563.pep TIY YAAQQSSIDIQAAHNKLNSNTTQTYEQKGLTVAFSSPVTDLAQQA-IAVAQSSKQVGQSKN 1620 1630 1640 1650 1660 1670 1680 1690 1700 1710 1720 1470 1480 1490 1500 1510 1520 g563.pep QIQAGGKTTLYCRRCGEQSNINITGSGVSGRAGTGLIADKQIHLQSAEQSNTERSQNKSA Mlllllhll 1 = 1 = 111 MlhM 1111111 M I h 11111 m563.pep QIQAGGKTTLIATGAAEQSNINIAGSDVAGKAGTILIADNDITLQSAEQSNTERGQNKSA 1730 1740 1750 1760 1770 1780 1530 1540 1550 1560 1570 1580 g563. pep GWNAGAAVSFGQGGWSLGVAAGGNVGKGYGYGDSVTHRHSHIGDKGSQTLIQSGGDTIIK 111 = 1111 E i 1111111111111 E 11 II m563. pep GWNAGAAVSFGQGGWSLGVTAGGNVGKGYGNGDSITHRHSHIGDKGSQTLIQSGGDTTIK 1790 1800 1810 1820 1830 1840 1590 1600 1610 1620 1630 1640 g563.pep GAQVRGKGVQVNAKNLSIQSVQDRETYQSKQQNAGAQVTVGYGFSASGDYSQSKIRADHA m563.pep GAQVRGKGVQVNAKNLS I QSVQDRETYQS KQQNASAQVTVGYGFSAGGDYSQS KI RADHV 1850 1860 1870 1880 1890 1900 1650 1660 1670 1680 1690 1700 g563. pep SVTEQSGIYAGEDGYQIKVGNHTGLKGGIITSSQSAKDKGKNRFSTGTLAGSDIQNYSQY MMMMMMMIMMMM II I II 11 h II h II II 11 h h I h llhhlll m563. pep SVTEQSGIYAGEDGYQIKVGNHTDLKGGIITSTQSAEDKGKNRFQTATLTHSDIKNHSQY 1910 1920 1930 1940 1950 1960 1710 1720 1730 1740 1750 1760 g563. pep EGKSFGLGASVAVSGKTLGQGAKNKPQDKHLTSIADKNGASSSVGYGSDSDSQSSITKSG m563. pep KGESFGLGASASISGKTLGQGAQNKPQNKHLTSVADKNSASSSVGYGSDSDSQSSITKSG 1970 1980 1990 2000 2010 2020 1770 1780 1790 1800 1810 1820 g563.pep INTPKNIQITDEAAQIRLTGKIAAQTKADIDTNVTTDTAERHSGSLKNIFDKDRVQSELD III MMMIMMMMM II II 11 II II I II II II II II 11 II I \A ~ - MMM m563.pep INT-RNIQITDEAAQIRLTGKTAAQTKADIDTNVTTDTAERHSGSLKNTFNKEAVQSELD 0 2040 2050 2060 2070 2080 1830 1840 1850 1860 1870 1880 g563.pep LQRTVSQDFSKNVQQTNTEINQHLDKLKADKEAAETAAAEALANGDMETAKRKAHEAQDA m563.pep LQRTVSQDFSKNVQQANTEINQHLDKLKADKEAAETAAAEALANGDMETAKRKAHEAQDA 190 2100 2110 2120 2130 2140 1890 1900 1910 1920 1930 1940 g563.pep AAKADNWQQGKVILNMLASGLAEPTQSGAGIAAATASPDVSYAIGQHFKDLAGQNANGKL MMMMMMMMMMM MMMMIMMM MMMMMMMIMMM m563.pep AAKADNWQQGKVILNMLASGLAAPTQSGAGIAAATASPAVSYAIGQHFKDLAGQNANGKL 2150 2160 2170 2180 2190 2200 1950 1960 1970 1980 1990 2000 g563. pep TASQETAHVLAHAVLGAAVAAAXGNNAPAGALGAGGSEAAAPIIGKWLYGKGDGGSLNAE M M M M M M I M M M I h I I I M I h l l 1 1 1 I I I I h l l l l l l h M M I m563.pep TASQETAHVLAHAVLGAAVAAVGDNNALAGALSAGGSEAAAPYISKWLYGKEKGSDLTAE 2210 2220 2230 2240 2250 2260 2010 2020 2030 2040 2049 g563.pep EKETVSAITRMLGTAAGAAEGNSSADAVWGCFQTASDFASSFSYPINMX M l l h l l l M M M h M h I ■ ■ ■ ■ ■ ■ I m563. pep EKETVTAITNVLGTATGAAVGNSATDAAQGSLNAQSAVENNDTVEQVKFALRHPRIAIAI 2270 2280 2290 2300 2310 2320 m563. pep GSVHKDPGSTLEPNISTIASTFQLNLFPNSEFGGEGGVGNAFRHVLWQATITREFGKDIA 2330 2340 2350 2360 2370 2380 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1669>: m564. seq 1 ATGAACCGCA CCCTGTACAA AGTTGTATTT AACAAACATC GAAACTGCAT 51 GATAGCCGTT GCTGAAAATG CCAAACGCGA GGGCAAAAAC ACAGCCGACA 101 CCCAAGCTGT AGGTATTTTG CCAAATGATA TTGCGGGCTT TGCGGGTTTT 151 ATCCATTCTA TCTCTGTTAT CTCATTCTCC CTTTCATTAC TGCTCGGTTC 201 TGCCCTTATC CTGACTTCTT CTTCTGCTAC TGCCCAAGGT ATCGTTGCCG 251 ACAAATCCGC ACCTGCACAG CAACAGCCTA CCATCCTGCA AACAGGTAAC 301 GGCATACCGC AAGTCAATAT TCAAACCCCT ACTTCGGCAG GGGTTTCTGT 351 TAATCAATAC GCCCAGTTTG ATGTGGGTAA TCGCGGGGCG ATTTTAAACA 401 ACAGTCGCAG CAACACCCAA ACACAGCTAG GCGGTTGGAT TCAAGGCAAT 451 CCTTGGTTGG CAAGGGGCGA AGCACGTGTG GTTGTAAACC AAATCAACAG 501 CAGCCATTCT TCACAACTGA ATGGCTATAT TGAAGTGGGC GGACGACGTG 551 CAGAAGTCGT TATTGCCAAT CCGGCAGGGA TTGCAGTCAA TGGTGGTGGT 601 TTTATCAATG CTTCCCGTGC CACTTTGACG ACAGCCCAAC CGCAATATCA 651 AGCAGGAGAC CTTAGCGGCT TTAAGATAAG GCAAGGCAAT GTTGTAATCG 701 CCGGACACGG TTTGGATGCA CGTGATACCG ATTACACACG TATTCTCAGT 751 TATCATTCCA AAATTGATGC ACCCGTATGG GGACAAGATG TTCGTGTCGT 801 CGCGGGACAA AACGATGTGG CCGCAACAGG TGATGCACAT TCGCCTATTC 851 TCAATAATGC TGCTGCCAAT ACGTCAAACA ATACAGCCAA CAACGGCACA 901 CATATCCCTT TATTTGCGAT TGATACAGGC AAATTAGGAG GTATGTATGC 951 CAACAAAATC ACCTTGATCA GTACGGTCGA GCAAGCAGGC ATTCGTAATC 1001 AAGGGCAATG GTTTGCCTCA GCCGGCAATG TGGCAGTGAA TGCTGAGGGT 1051 AAACTGGTCA ACACGGGCAT GATTGCAGCG ACGGGAGAAA ATCATGCGGT 1101 TTCACTTCAT GCCCGCAATG TTCATAATAG CGGTACGGTT GCCTCACAGG 1151 ATGATGCCAA TATTCACAGC CAGACGCTGG ACAATTCAGG TACGGTCTTA 1201 TCCTCAGGTC GATTGACTGT TCGTAATTTA GGCCGTCTGA AAAACCAAAA 1251 CAACGGTACG ATCCAGGCTG CCCGCTTAGA TATGTCAACA GGTGGTTTGG 1301 ATAACACAGG TAATATTACT CAAACAGGTT CACAAGCATT GGATTTGGTA 1351 TCTGCCGGCA AATTCGATAA CAGTGGCAAG ATTGGTGTAA GTGACGTTCC 1401 ACAGACCGGT TTGAATCCCA ATCCATCAGT CATACCACAG ATTCCGAGTA 1451 CTGCAACAGG TTCAGGCAGC AGCACTGTCT CGGTATCTAA GCCTGGTTCA 1501 AACAATCCCG TTTCACCTAC AGCACCTGCA AAAAACTACG CCGTAGGACG 1551 CATTCAAACA ACAGGAGCAT TTGACAATGC AGGATCAATT AATGCGGGTG 1601 GGCAAATTGA CATTGCCGCC CAAAACGGTT TGGGAAATTC GGGTAGTCTG 1651 AATGCGGCTA AACTACGAGT ATCAGGCGAT TCATTTAACA ATACGGTAAA 1701 AGGCAAACTC CAGGCACACG ATCTGGCTGT TAACACTCAA ACTGCTAAAA 1751 ACAGCGGTCA CTTATTAACT CAAACCGGCA AGATTGATAA CCGTGAACTG 1801 CATAATGCCG GAGAAATTGC CGCCAACAAT CTGACACTCA TTCATTCGGG 1851 CCGCTTGAGC AATGATAAAA AAGGCAATAT TCGAGCTGCA CATTTACAGC 1901 TTGATACCGC CGGTTTACAT AATGCAGGTA ACATTCTTGC CGATAGTGGA 1951 ACCGTTACCA CCAAGAATAA TCTTCGCAAT ACAGGAAAAG TTTCTGTTGC 2001 ACGACTGAAT ACCGAAGGTC AGACTCTAGA TAATACGCGC GGACGTATAG 2051 AGGCTGAAAC GGTTAACATC CAAAGTCAGC AACTGACTAA CCAAAGCGGC 2101 CATATTACTG CTACCGAACA ACTGACTATC AATAGTCGAA ATGTAGACAA 2151 CCAAAACGGC AAACTCCTAT CTGCAAACCA AGCACAATTA GCTGTTTCAG 2201 ACGGCCTATA CAACCAACAT GGTGAAATTG CCACCAACCG GCAGTTGTCT 2251 ATTCACGATA AAAATCAAAA CACTTTGGCG TTAAACAATG CGGATGGCAC 2301 GATTCAATCT GCCGGTAATG TATCGCTACA AGCCAAATCA CTCGCCAACA 2351 ATGGCACATT AACAGCCGGT AACAAACTGG ATATTGCTTT GACGGACGAT 2401 TTCGTCGTAG AGCGCGACCT CACTGCAGGC AAACAATTAA ATCTAAGCAT 2451 AAAAGGCCGT CTGAAAAATA CCCATACCCT ACAAGCAGGC CATACGCTCA 2501 AACTCAATGC CGGCAATATA GATAACCAAG TTACAGGCAA AATTATTGGT 2551 GGAGAACAAA CGGACATCAC ATCCGAACAG CATGTTGACA ACAGGGGCTT 2601 GATCAACAGC GACGGTTTGA CCCACATCGG TGCAGGTCAA ACCCTGACCA 2651 ACACCGGGAC AGGCAAAATC TATGGCAACC ATATTGCCCT GGACGCGCAA 2701 ATACTGCTTA ACCGGGAAGA AACGACGGAA GGCAGTACCA AAGCGGGGGC 2751 AAT GCTGCA AGGAAACGTT TGGATATTGG AGCGAAAGAG ATTCATAACC 2801 AAGAAGGTGC CCTACTATCC AGCGAAGGTA TTTTTGCCGT AgGTAATCGA 2851 CTGGATGAAC AACATCATGC GGCAGGCATG GCCGATACCT TTGTTAATGG 2901 CAGTGCCGGT TTGGAAGTAC AAGGTGATGC ATTGATGTCC GTTCGGAATA 2951 TGCAGAATAT CAATAATCAC TTTAAAACAG AGACATACTT AGCCAAAGCG 3001 GAAAAGCAAG TCCGCGACTA CACCGTACTG GGGCAAAATA CCTACTATCA 3051 GGCGGGAAAA GACGGTTTAT TCGACAACTC GCAAGGACAA AAAGACCAAA 3101 CTACTGCTAC GTTCCATTTA AAAAATGGTT CTCGTATTGA GGCCAACCAA 3151 TGGCATGTCC GAGACTACCA CATCGAGACT TATAAAGAAC GCATCATCGA 3201 AAACCGGCCG GCACACATTA CTGTGGGCGG TGATTTGACT GCCTCAGGTC 3251 AAAATTGGCT GAACAAAGAC AGCCGGATTG TAGTAGGCGG GCGTATTATC 3301 ACTGATGATT TAAACCAGAA AGAAATTACC AATCAAAGTA CAACAGGCAA 3351 AGGTCGCACA GATGCTGTCG GCACACAGTG GGATTCAGTT ACAAAAAAAG 3401 GATGGTACAG CGGTAGAAAA AGACAACGCC GTACTGAAAG AAACCATACT 3451 CCTTACCATG ATACCCAACT ATTTACCCAC GACTTCGACA CGCCTGTATC 3501 CGTCATCCAA CAGAATGCCG CCTCCCCTTC CTTTCAACCC GCCGCATCTG 3551 CAATCAAACT GATTGACGGA GTATCCACGG CAGCCGTCAA TGGTCAGCGC 3601 ATCCATACCG GTAATGTGGT CTCGTTAAAT AACGCTACTG TTACTCTGCC 3651 TAACAGCAGC CTCTATACCA CCCATCCTGA CAATAAAGGC TGGTTGGTTG 3701 AAACCGATCC TCAATTTGCA GACTACCGCC GCTGGTTGGG CAGCGACTAC 3751 ATGTTGCAAC AACTGCAATT GGACACCAAT CATCTACACA AACGGCTTGG 3801 CGACGGCTAC TACGAACAAA AACTTGTTAA TGAACAAATC CATCAGTTAA 3851 CAGGCTACCG CCGACTCGAC GGCTACAGGA GTGATGAAGA ACAATTCAAA 3901 GCTCTGATGG ACAACGGCCT TACTGCTGCC AAAACATTCG GTCTCACCCC 3951 AGGTATCGCC TTGAGTGCAG AGCAAGTTGC CCGCTTAACT TCAGATATCG 4001 TTTGGATGGA AAATCAAACC GTCACCCTGT CTGACGGTTC GACTCAAACC 4051 GTACTGGTTC CTAAAGTCTA TGCCCTGGCG CGCAAAGGTG ATCTCAATAC 4101 CTCCGGTGGC CTGATTAGTG CCGAACAAGT CTTACTTAAA CTGCAAAACG 4151 GCAACCTGAC TAACAGCGGT ACCATTGCGG GGCGACAGGC CGTACTCATC 4201 CAGGCACGGA ATATTAACAG CAACGGTAAC ATTCAAGCCG ACCAAATCGG 4251 CTTAAAAGCT GAAAAAAGTA TCAATATCGA CGGCGGGCAG GTACAAGCAG 4301 GCAGACTGCT GACTGCCCAA GCGCAAAATA TCAACCTTAA CGGTACAACC 4351 CAAACTTCCG GTAATGAACG TAACGGCAAT ACCGCCATCG ATCGTATGGC 4401 CGGCATTAAC GTGGTCGGAA GCCATACTGA ACAAGTAGAT AACAGAACTT 4451 CAGACGGCAT CCTATCCCTG CATGCCAGCA ACGATATCAA CCTCAATGCG 4501 GCCACCGTCT CTAACCAAGT TAAAGACGGC ACTACCCAAA TTACCGCCGG 4551 CAATAATCTC AACCTCGGCA CCATCCGTAC CGAACATCGC GAAGCCTATG 4601 GTACATTAGA TGACGAGAAC CATCGCCATG TCCGCCAAAG TACCGAAGTC 4651 GGCAGCAGTA TCCGCACGCA AAACGGCGCA CTGCTTAGAG CCGGTAACGA 4701 CTTAAAAATC CGCCAAGGCG AACTGGAGGC CGAAGAAGGC AAAACCGTCC 4751 TTGCCGCAGG ACGTGATGTC ACTATCAGCG AAGGACGCCA AATAACCGAA 4801 CTGGATACCT CGGTAAGCGG AAAAAGCAAA GGCATCCTTT CCAGTACCAA 4851 AACACACGAC CGCTACCGCT TCAGTCATGA TGAAGCAGTC GGCAGCAACA 4901 TCGGCGGCGG CAAAATGATT GTTGCAGCCG GGCAGGATAT CAATGTACGC 4951 GGCAGCAACC TTATTTCTGA TAAGGGCATT GTTTTAAAAG CAGGACACGA 5001 CATCGATATT TCTACTGCCC ATAATCGCTA TACCGGCAAT GAATACCACG 5051 AGAGCAAAAA ATCAGGCGTC ATGGGTACTG GCGGATTGGG CTTTACTATC 5101 GGTAACCGGA AAACTACCGA TGACACTGAT CGTACCAATA TTGTCCATAC 5151 AGGCAGCATT ATAGGCAGCC TGAATGGAGA CACCGTTACA GTTGCAGGAA 5201 ACCGCTACCG ACAAACCGGC AGTACCGTCT CCAGCCCCGA GGGGCGCAAT 5251 ACCGTCACAG CCAAAAGCAT AGATGTAGAG TTCGCAAACA ACCGGTATGC 5301 CACTGACTAC GCCCATACCC AGGAACAAAA AGGCCTTACC GTCGCCCTCA 5351 ATGTCCCGGT TGTCCAAGCT GCACAAAACT TCATACAAGC AGCCCAAAAT 5401 GTGGGCAAAA GTAAAAATAA ACGCGTTAAT GCCATGGCTG CAGCCAATGC 5451 TGCATGGCAG AGTTATCAAG CAACCCAACA AATGCAACAA TTTGCTCCAA 5501 GCAGCAGTGC GGGACAAGGT CAAAACAACA ATCAAAGCCC CAGTATCAGT 5551 GTGTCCATTA CCTACGGCGA ACAGAAAAGT CGTAACGAGC AAAAAAGACA 5601 TTACACCGAA GCGGCAGCAA GTCAAATTAT CGGCAAAGGG CAAACCACAC 5651 TTGCGGCAAC AGGAAGTGGG GAGCAGTCCA ATATCAATAT TACAGGTTCC 5701 GATGTCATCG GCCATGCAGG TACTGCCCTC ATTGCCGACA ACCATATCAG 5751 ACTCCAATCT GCCAAACAGG ACGGCAGCGA GCAAAGCAAA AACAAAAGCA 5801 GTGGTTGGAA TGCAGGCGTA GCCGTCAAAA TAGGCAACGG CATCAGGTTT 5851 GGAATTACCG CCGGAGGAAA TATCGGTAAA GGTAAAGAGC AAGGGGGAAG 5901 TACTACCCAC CGCCACACCC ATGTCGGCAG CACAACCGGC AAAACTACCA 5951 TCCGAAGCGG CGGGGATACC ACCCTCAAAG GTGTGCAGCT CATCGGCAAA 6001 GGCATACAGG CAGATACGCG CAACCTGCAT ATAGAAAGTG TTCAAGATAC 6051 TGAAACCTAT CAGAGCAAAC AGCAAAACGG CAATGTCCAA GTTACTGTCG 6101 GTTACGGATT CAGTGCAAGC GGCAGTTACC GCCAAAGCAA AGTCAAAGCA 6151 GACCATGCCT CCGTAACCGG GCAAAGCGGT ATTTATGCCG GAGAAGACGG 6201 CTATCAAATC AAAGTCAGAG ACAACACAGA CCTCAAGGGC GGTATCATCA 6251 CGTCTAGCCA AAGCGCAGAA GATAAGGGCA AAAACCTTTT TCAGACGGCC 6301 ACCCTTACTG CCAGCGACAT TCAAAACCAC AGCCGCTACG AAGGCAGAAG 6351 CTTCGGCATA GGCGGCAGTT TCGACCTGAA CGGCGGCTGG GACGGCACGG 6401 TTACCGACAA ACAAGGCAGG CCTACCGACA GGATAAGCCC GGCAGCCGGC 6451 TACGGCAGCG ACGGAGACAG CAAAAACAGC ACCACCCGCA GCGGCGTCAA 6501 CACCCACAAC ATACACATCA CCGACGAAGC GGGACAACTT GCCCGAACAG 6551 GCAGGACTGC AAAAGAAACC GAAGCGCGTA TCTACACCGG CATCGACACC 6601 GAAACTGCGG ATCAACACTC AGGCCATCTG AAAAACAGCT TCGACAAAGA 6651 CGCGGTCGCC AAAGAGATCA ACCTGCAAAG GGAAGTAACG AAGGAGTTCG 6701 GCAGAAACGC CGCCCAAGCC GTAGCGGCCG TTGCCGACAA ACTCGGCAAT 6751 ACCCAAAGTT ACGAACGGTA TCAGGAAGCC CGAACCCTGC TGGAGGCCGA 6801 ACTGCAAAAC ACGGACAGCG AAGCCGAAAA AGCCGCCTTC CGCGCATCCC 6851 TCGGCCAAGT AAACGCCTAT CTTGCCGAAA ACCAAAGCCG CTACGACACC 6901 TGGAAAGAAG GCGGCATAGG CAGGAGCATA CTGCACGGGG CGGCAGGCGG 6951 ACTGACGACC GGCAGCCTCG GCGGCATACT GGCCGGCGGC GGCACTTCCC 7001 TTGCCGCACC GTATTTGGAC AAAGCGGCGG AAAACCTCGG TCCGGCGGGC 7051 AAAGCGGCGG TCAACGCACT GGGCGGTGCG GCCATCGGCT ATGCAACTGG 7101 TGGTAGTGGT GGTGCTGTGG TGGGTGCGAA TGTAGATTGG AACAATAGGC 7151 AGCTGCATCC GAAAGAAATG GCGTTGGCCG ACAAATATGC CGAAGCCCTC 7201 AAGCGCGAAG TTGAAAAACG CGAAGGCAGA AAAATCAGCA GCCAAGAAGC 7251 GGCAATGAGA ATCCGCAGGC AGATACTGCG TTGGGTGGAC AAAGGTTCCC 7301 AAGACGGCTA TACCGACCAA AGCGTCATAT CCCTTATCGG AATGAAAGGC 7351 GAAGACAAAG CCTTGGGTTA TACTTGGGAC TACCGCGACT ACGGCGCAAG 7401 AAATCCGCAA ACCTACAACG ATCCGAAGCT GTTTGAGGAA TACCGCCGAC 7451 AGGACAAACC CGAATACCGC AACCTGACCT GGCTGCACAG CGGGACGAAA 7501 GACACCAAAA TCAGGCAGGG AGAGCGGAAA AACGAAGAGT TTGCACTGAA 7551 CGTTGCCGAA GGACTGACGA GCCTTGTCAA CCCCAATCCG AGGATAAAAG 7601 TCCCGATTCT TGCAGGCATC CGCAACCTGA AAAACATCAA GCCGACAGTT 7651 ACCGGCAGCG ATCCCTTATT GGCGGGTGCG GGGAATATCC GTATCCCTGC 7701 AAACGGCAAT GTTGCGAAGG GGGACAGGAT TCCGGATACG GCATTGGCTA 7751 GCAAGGGAAT CAAACATAAA GATCGTAAAG ATCAACTGGA GAAAAAATAA This corresponds to the amino acid sequence <SEQ ID 1670; ORF 564>: m564.pep 1 MNRTLYKWF NKHRNCMIAV AENAKREGKN TADTQAVGIL PNDIAGFAGF 51 IHSISVISFS LSLLLGSALI LTSSSATAQG IVADKSAPAQ QQPTILQTGN 101 GIPQVNIQTP TSAGVSVNQY AQFDVGNRGA ILNNSRSNTQ TQLGGWIQGN 151 PWLARGEARV WNQINSSHS SQLNGYIEVG GRRAE IAN PAGIAVNGGG 201 FINASRATLT TAQPQYQAGD LSGFKIRQGN WIAGHGLDA RDTDYTRILS 251 YHSKIDAPVW GQDVRWAGQ NDVAATGDAH SPILNNAAAN TSNNTANNGT 301 HIPLFAIDTG KLGGMYANKI TLISTVEQAG IRNQGQWFAS AGNVAVNAEG 351 KLVNTGMIAA TGENHAVSLH ARNVHNSGTV ASQDDANIHS QTLDNSGTVL 401 ΞSGRLTVRNL GRLKNQNNGT IQAARLDMST GGLDNTGNIT QTGSQALDLV 451 SAGKFDNSGK IGVSDVPQTG LNPNPSVIPQ IPSTATGSGS STVSVSKPGS 501 NNPVSPTAPA KNYAVGRIQT TGAFDNAGSI NAGGQIDIAA QNGLGNSGSL 551 NAAKLRVSGD SFNNTVKGKL QAHDLAVNTQ TAKNSGHLLT QTGKIDNREL 601 HNAGEIAANN LTLIHSGRLS NDKKGNIRAA HLQLDTAGLH NAGNILADSG 651 TVTTKNNLRN TGKVSVARLN TEGQTLDNTR GRIEAETVNI QSQQLTNQSG 701 HITATEQLTI NSRNVDNQNG KLLSANQAQL AVSDGLYNQH GEIATNRQLS 751 IHDKNQNTLA LNNADGTIQS AGNVSLQAKS LANNGTLTAG NKLDIALTDD 801 FWERDLTAG KQLNLSIKGR LKNTHTLQAG HTLKLNAGNI DNQVTGKIIG 851 GEQTDITSEQ HVDNRGLINS DGLTHIGAGQ TLTNTGTGKI YGNHIALDAQ 901 ILLNREETTE GSTKAGAIAA RKRLDIGAKE IHNQEGALLS SEGIFAVGNR 951 LDEQHHAAGM ADTFVNGSAG LEVQGDALMS VRNMQNINNH FKTETYLAKA 1001 EKQVRDYTVL GQNTYYQAGK DGLFDNSQGQ KDQTTATFHL KNGSRIEANQ 1051 WHVRDYHIET YKERIIENRP AHITVGGDLT ASGQNWLNKD SRIWGGRII 1101 TDDLNQKEIT NQSTTGKGRT DAVGTQWDSV TKKGWYSGRK RQRRTERNHT 1151 PYHDTQLFTH DFDTPVSVIQ QNAASPSFQP AASAIKLIDG VSTAAVNGQR 1201 IHTGNWSLN NATVTLPNSS LYTTHPDNKG WLVETDPQFA DYRRWLGSDY 1251 MLQQLQLDTN HLHKRLGDGY YEQKLVNEQI HQLTGYRRLD GYRSDEEQFK 1301 ALMDNGLTAA KTFGLTPGIA LSAEQVARLT SDIVWMENQT VTLSDGSTQT 1351 VLVPKVYALA RKGDLNTSGG LISAEQVLLK LQNGNLTNSG TIAGRQAVLI 1401 QARNINSNGN IQADQIGLKA EKSINIDGGQ VQAGRLLTAQ AQNINLNGTT 1451 QTSGNERNGN TAIDRMAGIN WGSHTEQVD NRTSDGILSL HASNDINLNA 1501 ATVSNQVKDG TTQITAGNNL NLGTIRTEHR EAYGTLDDEN HRHVRQSTEV 1551 GSSIRTQNGA LLRAGNDLKI RQGELEAEEG KTVLAAGRDV TISEGRQITE 1601 LDTSVSGKSK GILSSTKTHD RYRFSHDEAV GSNIGGGKMI VAAGQDINVR 1651 GSNLISDKGI VLKAGHDIDI STAHNRYTGN EYHESKKSGV MGTGGLGFTI 1701 GNRKTTDDTD RTNIVHTGSI IGSLNGDTVT VAGNRYRQTG STVSSPEGRN 1751 TVTAKSIDVE FANNRYATDY AHTQEQKGLT VALNVPWQA AQNFIQAAQN 1801 VGKSKNKRVN AMAAANAAWQ SYQATQQMQQ FAPSSSAGQG QNNNQSPSIS 1851 VSITYGEQKS RNEQKRHYTE AAASQIIGKG QTTLAATGSG EQSNINITGS 1901 DVIGHAGTAL IADNHIRLQS AKQDGSEQSK NKSSGWNAGV AVKIGNGIRF 1951 GITAGGNIGK GKEQGGSTTH RHTHVGSTTG KTTIRSGGDT TLKGVQLIGK 2001 GIQADTRNLH IESVQDTETY QSKQQNGNVQ VTVGYGFSAS GSYRQSKVKA 2051 DHASVTGQSG IYAGEDGYQI KVRDNTDLKG GIITSSQSAE DKGKNLFQTA 2101 TLTASDIQNH SRYEGRSFGI GGSFDLNGGW DGTVTDKQGR PTDRISPAAG 2151 YGSDGDSKNS TTRSGVNTHN IHITDEAGQL ARTGRTAKET EARIYTGIDT 2201 ETADQHSGHL KNSFDKDAVA KEINLQREVT KEFGRNAAQA VAAVADKLGN 2251 TQSYERYQEA RTLLEAELQN TDSEAEKAAF RASLGQVNAY LAENQSRYDT 2301 WKEGGIGRSI LHGAAGGLTT GSLGGILAGG GTSLAAPYLD KAAENLGPAG 2351 KAAVNALGGA AIGYATGGSG GAWGANVDW NNRQLHPKEM ALADKYAEAL 2401 KREVEKREGR KISSQEAAMR IRRQILRWVD KGSQDGYTDQ SVISLIGMKG 2451 EDKALGYTWD YRDYGARNPQ TYNDPKLFEE YRRQDKPEYR NLTWLHSGTK 2501 DTKIRQGERK NEEFALNVAE GLTSLVNPNP RIKVPILAGI RNLKNIKPTV 2551 TGSDPLLAGA GNIRIPANGN VAKGDRIPDT ALASKGIKHK DRKDQLEKK* Computer analysis of this amino acid sequence gave the following results: Homology with fha m564/fha ID FHAB_BORPE STANDARD; PRT; 3591 AA. AC P12255; DT 01-OCT-1989 (REL. 12, CREATED) DT 01-FEB-1996 (REL. 33, LAST SEQUENCE UPDATE) DT 01-FEB-1996 (REL. 33, LAST ANNOTATION UPDATE) DE FILAMENTOUS HEMAGGLUTININ. SCORES Initl: 190 Initn: 524 Opt: 594 Smith-Waterman score: 866; 21.7% identity in 2427 aa overlap 10 20 30 40 50 60 m564 .pep MNRTLYKWFNKHRNCMIAVAENAKREGKNTADTQAVGILPNDIAGFAGFIHSISVISFS I I : I I :: I I:: I: :: I : I: I II :: I : : I : | :: ::: fhab_borpe MNTNLYRLVFSHVRGMLVPVSEHCTV-G-NTFCGRTRG QARSGARATSLSVAPNALA 10 20 30 40 50 70 80 90 100 110 119 m564 . pep LSLLLG-SALILTSSSATAQGIVADKSAPAQQQPTILQTGNGIPQVNIQTPTSAGVSVNQ : I : I : : : I I : : I M I I I : I I I I : I I I I I : I : I I I I : fhab_borpe WALMLACTGLPLVTH AQGLV P-QGQTQVLQGGNKVPWNIADPNSGGVSHNK 60 70 80 90 100 120 130 140 150 160 170 179 m564 . pep YAQFDVGNRGAILNNSRSNTQTQLGGWIQGNPWLARGEARVWNQINSSHSΞQLNGYIEV : M M I :: : II : :: : :: I I : II Ml : I :: : : : : : : | : | | : || fhab_borpe FQQFNVANPGWFNNGLTDGVSRIGGALTKNPNLTR-QASAILAEVTDTSPSRLAGTLEV 110 120 130 140 150 160 180 190 200 210 220 230 239 m564 . pep GGRRAEWIANPAGIAVNGGGFINASRATLTTAQPQYQAGDLSGFKIRQGNWIAGHGLD I : I : :: I I I I M M : MM I I I I :: I : :: I : I : : : II : I : I I :: fhab_borpe YGKGADLI IANPNGISVNGLSTLNASNLTLTTGRPSVNGGRI-GLDVQQGTVTIERGGVN 170 180 190 200 210 220 240 250 260 270 280 290 m564 . pep ARDTDYTRILSYHSKIDAPV WGQ DVRWAGQNDVAATGDAHSPILNNAAANTSN I I : : : I : : : I I : I : I I I I I : : I I I I : : : fhabjDorpe ATGLGYFDWARLVKLQGAVSSKQGKPLADIAWAGANRYDHATRRATPI AAGARG 230 240 250 260 270 280 300 310 320 330 340 350 m564 .pep NTANNGTHIPLFAIDTGKLGGMYANKITLISTVEQAGIRNQGQWFASAGNVAVNAEGKLV fhab borpe AAAGA- -YAIDGTAAGAMYGKHITLVSSDSGLGVRQLGS-LSSPSAITVSSQGEIA 290 300 310 320 330 360 370 380 390 400 410 m564 . pep NTGMIAATGENHAVSLHARNVHNSGTVASQDDANIHSQTLDNSGTVLSSGRLTVRNLGRL : II : : I I : : : I :: I : II I :: ::|:| : : : : : I I fhab_borpe LGDATVQRGPLSLKGAGWSAGKLASGGGAV NVAGGGAVKIA SASSVGNL 340 350 360 370 380 420 430 440 450 460 470 m564 . pep KNQNNGTIQAARLDMSTGGLDNTGNITQTGSQALDLVSAGKFDNSGKIGVSDVPQTGLNP I :: I : I I: I : : I :: : I I |:: I : I :: : : : : : I : fhab_borpe AVQGGGKVQATLLNAG GTLLVSGRQAVQLGAASSRQALSVNAGGALKADKLSA 390 400 410 420 430 480 490 500 510 520 530 m564 .pep NPSV-IPQIPSTATGSGSSTVSVSKPGSNNPVSPTAPAKNYAVGRIQTTGAFD-NAGSIN : I : :: I I |: M :: : I : : I | : ||::: | : || : fhab_borpe TRRVDVDGKQAVALGSASSNALSVRAGGA LKAGKLSATGRLDVDGKQAVTLGSVA 440 450 460 470 480 490 540 550 55 560 570 579 m564 . pep AGGQIDIAAQNGLGNSGSLNAAKLRVSG DSFNNT VKGKLQAHDLAVNT : I :: :: I : : I : :: : I : I : I I I : : : : : I I I : : I : fhab_borpe SDGALSVSAGGNLRANELVSSAQLEVRGQREVALDDASSARGMTWAAGALAARNLQSKG 500 510 520 530 540 550 580 590 600 610 620 630 m564 . pep QTAKNSGHLLTQTGKIDNRELH—NAGEIAANNLTLIHSGRLSNDKKGNIRAAHLQLDTA : :: I : : : : : : : II : : I : : :: I : ::: : I : : : I I I : : I : fhabjoorpe AIGVQGGEAVSVANANSDAELRVRGRGQVDLHDLSAARGADISGEGRVNIGRARSDSDVK 560 570 580 590 600 610 640 650 660 670 680 690 m564 . pep GLHNAGNILADSGTVTTKNNLRNTGKVSVARLNTEGQTLDNTRGRIEAETVNIQSQQLTN : I : M ::: IM M : :: I : : I : I I : : I fhabjaorpe -VSAHGALSIDSMTALGAIGVQAGGSVSAKDMRSRGAVTVSGGG AVNLGDVQ 620 630 640 650 660 700 710 720 730 740 750 m564 . pep QSGHITATEQLTINSRNVDNQNGKLLSANQAQLAVSDGLYNQHGEIATNRQLSIHDKNQN : : I : : II : : : I : I I I : I I :: I I I : : :::: :: fhabjoorpe SDGQVRATSAGAMTVRDV AAAADLALQAGDALQAGFLKSAGAMTVNGRDAV 670 680 690 700 710 760 770 780 790 800 810 m564 .pep TLALNNADGTIQSAGNVSLQAKSLANNGTLTAGNKLDIALTDDFWERDLTAGKQL-NLS I I I : ::: I :: :: : : I I : I : I : : I : : : : I : I MM fhabjoorpe RL DGA-HAGGQLRVSSDGQAALGSLAAKGELTVSAARAATVA-EL KSLDNIS 720 730 740 750 760 820 830 840 850 860 870 m564 .pep IKGRLK-NTHTLQAGHTLKLNA-GNIDNQVTGKIIGGEQTDITSEQHVDNRGLINSDGLT : I : :::::::: : :: | | : | : | |: : :: I I : I ::: | : | fhab_borpe VTGGERVSVQSVNSASRVAISAHGALD VGKV--SAKSGIGLE GWGAVGADSL- 770 780 790 800 810 880 890 900 910 920 930 m564 .pep HIGAGQTLTNTGTGKIYGNHIALDAQILLNREETTEGSTKAGAIAARKRLDI-GAKEIHN I : : : : : I : :: I MM: I I : I : I : I : : : fhabjoorpe --GSDGAISVSGRDAVRVDQARSLADISLG AEGGATLGAVEAAGSIDVRGGSTV-- 820 830 840 850 860 940 950 960 970 980 990 m564 . pep QEGALLSSEGIFAVGNRLDEQHHAAGMADTFVNGSAGLEVQGDALMSVRNMQNINNHFKT fhabjoorpe AANSLHANRDVRVSGK—DAVRVTAATSGGGLHVSSGRQLDLGAVQA-RGALALDGGAGV 870 880 890 900 910 920 1000 1010 1020 1030 1040 1050 m564 . pep ETYLAKAEK—QVRDYTVLGQNTYYQAGKDGLFDNSQGQKDQTTATFHLKNGSRIEANQ- III : I : I M : I : : I I : I : I : :: I : : fhabjoorpe ALQSAKASGTLHVQGGEHLDLGTLAAVGAVDV NGTGDVRVAKLVSDAGADLQAGRS 930 940 950 960 970 1060 1070 1080 1090 1100 m564 .pep —WHVRDYHIETYKERIIENRPAHITVGGDLTASGQNWLNKDSRIWGGRIITDDLNQKE : I : : : : : : | | |:: : | : : | : | : :: : | fhabjoorpe MTLGIVDTTGDLQARAQQKLELGSVKSDGGLQAAAGGALSLAAAEVAGALELS GQGV 980 990 1000 1010 1020 1030 1110 1120 1130 1140 1150 1160 m564 . pep ITNQSTTGKGRTDAVGTQWDSVTKKGWY—SGRKRQRRTERNHTPYHDTQLFTHDFDTPV ::::::::: I I :: I : : : I I : : : I I I : I Mi ll fhabjoorpe TVDRASASRARIDSTGSVGIGALKAGAVEAASPRRARRALR QDFFTPG 1040 1050 1060 1070 1080 1170 1180 1190 1200 1210 1220 m564 .pep SVI QQNAASPSFQPAASAIKLIDGVSTAAVNGQRIHTGNWSLNNATVTLPNSSLYT II: I I:: M : : : I : I : I : I : MMM: : I fhabjoorpe SVWRAQGNVTVGRGDPHQGVLAQGDIIMDA--KGGTLLLRNDALTENGTVTISADSAVL 1090 1100 1110 1120 1130 1140 1230 1240 1250 1260 1270 1280 m564 . pep THPDNKGWLVETD-PQFADYRRWLGSDYMLQQLQLDTNHLHKRLGDGYYEQKLVNEQIHQ I : : : :: : | : | : :: | | | : :: | : :: : :: || fhab_borpe EHSTIESKISQSVLAAKGDKGKPAVSVKVAKKLFL—NGTLRAVNDN—NETMSGRQIDV 1150 1160 1170 1180 1190 1290 1300 1310 1320 1330 1340 m564 . pep LTGYRRLDGYRSDEEQFKALMDNGLTAAKTFGLTPG-IALSAEQVARLTSDIVWMENQTV : I : : : I : I I ::::: :: I |:: I ::: :: I : I : fhabjoorpe VDGRPQI TDAVTGEARKDESWSDAALVADGGPIWEAGELVSHAGGIGNGRNK— 1200 1210 1220 1230 1240 1250 1350 1360 1370 1380 1390 1400 m564 . pep TLSDGSTQTVLVPKVYALARKGDLNTSGGLISAEQVLLKLQNGNLTNSGTIAGRQAVLIQ : I : : II : I : I : : I : : : : I : I : : : I II I : :: : I : fhabjoorpe —ENGASVTVRTT GNLVNKGYISAGKQGVLEV-GGALTNEFLVGSDGTQRIE 1260 1270 1280 1290 1300 1410 1420 1430 1440 1450 m564 .pep ARNINSNGNIQ ADQIGLKAEKSINIDGGQVQAGRLLTAQ AQNINLNGTT fhabjoorpe AQRIENRGTFQSQAPAGTAGALWKAAEAIVHDGVMATKGEMQIAGKGGGSPTVTAGAKA 1310 1320 1330 1340 1350 1360 1460 1470 1480 1490 1500 m564 . pep QTSGNERNGNTAI-DRMAGINW-GSHTEQVDNRTSD-GILSLHASNDINLNAATVSNQV I I : I : : : : I I :: : :: I : I : I : : I : I : :: : : : :| :: : II fhabjoorpe TTSANKLSVDVASWDNAGSLDIKKGGAQVTVAGRYAEHGEVSIQGDYTVSADAIALAAQV 1370 1380 1390 1400 1410 1420 1510 1520 1530 1540 1550 m564 .pep —KDGTTQITAGNNLNLGT-IRTE HREAYGTLDDENHRHVRQST EVGS : I:::: I : :: ::: II : : I I ::: :: : I I :: : | : | : fhabjoorpe TQRGGAANLTSRHDTRFSNKIRLMGPLQVNAGGPVSNTGNLKVREGVTVTAASFDNETGA 1430 1440 1450 1460 1470 1480 1560 1570 1580 1590 1600 m564 .pep SIRTQNGALLRAGNDLKIRQGELEAEEGKTVLAAGRDV--TISEGRQITELDTS VSG fhabjoorpe EVMAKSATLTTSGAARN—AGKMQVKEAATIVAASVSNPGTFTAGKDITVTSRGGFDNEG 1490 1500 1510 1520 1530 1610 1620 1630 1640 1650 1660 m564 .pep K SKGILSSTKTHDRYRF SHDEAV-GSNIGGGKMIVAAGQDINVRGSNLISDKGI I : I I: : |: : I : I I : I :| : : :: : I |: |:: I::: : I :: fhabjoorpe KMESNKDIVIKTEQFSNGRVLDAKHDLTVTASGQADNRGSLKAGHDFTVQAQRI—DNSG 1540 1550 1560 1570 1580 1590 1670 16 1680 1690 1700 1710 m564 .pep VLKAGHDIDISTAHNRYTG NEYHESKKSGVMGTGGLGFTIGNRKTTDDTDRTNIV : : II II :: : I I I I : : I ::: : : I I : I : I : I II fhabjoorpe TMAAGHDATLKAPHLRNTGQWAGHDIHIINSAKLENTGRV—DARNDIALDVADFTN— 1600 1610 1620 1630 1640 1650 1720 1730 1 1740 1750 1760 1770 m564 .pep HTGSIIGSLNGDTVTVAGNRYRQT GSTVSSPEGRNTVTAKSIDVEFANNRYATDYA III: : :: I: I: I : I : : II I I II : : I : :: :: I fhabjoorpe -TGSLYAEHDA-TLTLAQGTQRDLWDQDHILPVAEGTLRVKAKSLTTEIETGNPGSLIA 1660 1670 1680 1690 1700 1710 1780 1790 1800 1810 1820 1830 m564 .pep HTQEQKGLTVALNVPWQAAQNFIQAAQNVGKSKNKRVNAMAAANAA-WQSYQATQQMQQ :: I I I : II | : : ::: I : : I : III I : : I : | : fhabjoorpe EVQE NIDNKQA IWGKDLTLS-SAHGNVANEANALLWAAGELTVKAQN 1720 1730 1740 1750 1840 1850 1860 1870 1880 1890 m564 .pep FAPSSSAGQGQNNNQSPΞISVSITYGEQKSRNEQKRHYTEAAASQIIGKGQTTLAATGSG :: : : I : : I : : I :: : I : I I : I :: | : | fhabjoorpe ITNKRAALIEAGGNARLTAAVALLNKLGRIRAGEDMHLD APRI ENTAKLSGEV 1760 1770 1780 1790 1800 1810 1900 1910 1920 1930 1940 1950 m564 .pep EQSNINITGSDVIGHAGTALIADNHIRLQSAKQDGSEQSKNKSSGWNAGVAVKIGNGIRF fhabjoorpe QRKGVQDVGGGEHGRWSGIGYVNYWLRAGNGKKAGT IAAPWYGGDLTAEQSLIEV 1820 1830 1840 1850 1860 I960 1970 1980 1990 2000 2010 m564 . pep GITAGGNIGKGKEQGGSTTHRHTHVGSTTGKTTIRSGGDTTLKGVQLIGKGIQADTRNLH I I I I : : III :: : I : : I I : I :: I : I : : fhab_borpe GKDLYLNAGARKDE HRHL LNEGVIQAGGHGHIGG DVDNRSV- 1870 1880 1890 1900 2020 2030 2040 2050 2060 m564 . pep IESVQDTETYQSKQQNGNVQVTVGYGFSASGSYRQSKVKA DHASVTGQSGIYAGE :: : I : I : :: : : : I : I : II : I : Ml fhabjoorpe VRTVSAMEYFKTPLPVSLTALDNRAGLSPATWNFQSTYELLDYLLDQNRYEYIWGLYPTY 1910 1920 1930 1940 1950 1960 2070 2080 2090 2100 2110 2120 m564 . pep DGYQIKVRDNTDLKGGIITSSQSAEDKGKNLFQTATLTASDIQNHS—RYEGRSFGIGGS : :: :: l ll l : : I I I : : : I : I I I :: : I fhabjoorpe TEWSVNTLKNLDL-GYQAKPAPTAPPMPKA PELDLRGHTLESAEGRKI-FGEY 1970 1980 1990 2000 2010 2130 2140 2150 2160 2170 m564 .pep FDLNGGWDGT VTDKQGRPTDRISPAAGYGSDGDSKNSTTRSGVNTHNIHITDEAG |:| :: : ::: |: I I : I :: : : : | ::::: : : fhabjoorpe KKLQGEYEKAKMAVQAVEAYGEATRRVHDQLG QRYGKALGGMDAETKEVDGIIQ 2020 2030 2040 2050 2060 2070 2180 2190 2200 2210 2220 2230 m564 .pep QLARTGRTAKETEARIYTGIDTETADQHSGHLKNSFDKDAVAKEINLQREVTKEFGRNAA fhabjoorpe EFAADLRTVYAKQADQAT-IDAET-DKVAQRYKSQID- -AVRLQAIQPGRVT--LAKALS 2080 2090 2100 2110 2120 2240 2250 2260 2270 2280 2290 m564 . pep QAVAAVADKLGNTQSYERYQEARTLLE-AELQNTDSEAEKAAFRASLGQVNAYL MM I I : : I : : I ::: : : : II : : I I Ml | : : fhabjoorpe AALGADWRALGHSQLMQRWKDFKAGKRGAEIAFYPKEQTVLAAGAGLTLSNGAIHNGENA 2130 2140 2150 2160 2170 2180 2300 2310 2320 2330 2340 2350 m564 .pep AENQSRYDTWKEGGIGRSILHGAAGGLTTGSLGGILAGGGTSLAAPYLDKAAENLGPAGK fhabjoorpe AQNRGRPEGLKIGAHSATSVSGSFDALRDVGLEKRLDIDDALAAVLVNPHIFTRIGAAQT 2190 2200 2210 2220 2230 2240 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1671>: g565. seq 1 atggacagca cattgtctaa aacgtgttgc gtttcgtgca tattgttgag 51 cgtaaccacc accattttcg cccgtcccag accggcggct tccaatactt 101 ccctgcgttt cgcatcgccg aacgacaccg gctcgcctgc acttctggct 151 acctgcacgc gtgcgatgtc caagtcgagc gcgaaatacg gaatatcctc 201 tttgggcgaa gacgcgtccg accgtctgcc cgcccctgcc gaagccgaca 251 atcagcacat gatcagactt gctcatcgct tccaccaaca tgctgtgcag 301 atcgagcgac ttcatgtccc agcttga This corresponds to the amino acid sequence <SEQ ID 1672; ORF 565.ng>: g565.pep 1 MDSTLSKTCC VSCILLSVTT TIFARPRPAA SΝTSLRFASP ΝDTGSPALLA 51 TCTRAMSKSS AKYGISSLGE DASDRLPAPA EADΝQHMIRL AHRFHQHAVQ 101 IERLHVPA* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1673>: m565. seq 1 ATGGACAGCA CATTGTCTAA AACGTGTTGC GTTTCGTGCA TATTGTTGAG 51 CGTAACCACC ACCATTTTCG CCCGTCCCAG ACCGGCGGCT TCCAATACTT 101 CCCTGCGTTT CGCATCGCCG AACGACACCG GTTCGCCCGC ACTTCTGGCA 151 ACCTGCACCC GCGCAATGTC CAAGTCGAGC GCGAAATACG GAATATCCTC 201 TTGGGCAAGG ACGCGTCCGA CCGTCTGCCC GCCCCTGCCG AAGCCGACAA 251 TCAGCACATG GTCGGACTTG CTCATGGTTT CTACCAGCAT ACTGTGCAGA 301 TCGAGCGACT TCATGTCCCA GCTTGACTTG ACCAAACGCC CGACCAGCGC 351 ATCGCTGCCG CCCAAGAGGA AGGGCGCGAT AATCATCGAC AGCAGAACCG 401 CCGCCGTCGC CGCCTGTTCC CATTCTGGCG AAACCATATC AAGCTGCCCG 451 GCAATGGCCA GCATCACGAA GCCGAACTCG CCGCCCTGCG CGAGATACAA 501 AGCCGTTTTG AGGCTGTCGC CGACCGAATG TTTCATTTTG AAGGCAATGG 551 CAAACACAAC CAGTGCCTTC AACACCAGCA GCATTGCCAA CAGCATCAAT 601 ACCTGCCGCC AGCCGCCGAT CAATGCCTGA This corresponds to the amino acid sequence <SEQ ID 1674; ORF 565>: m565.pep 1 MDSTLSKTCC VSCILLSVTT TIFARPRPAA SNTSLRFASP NDTGSPALLA 51 TCTRAMSKSS AKYGISSWAR TRPTVCPPLP KPTISTWSDL LMVSTSILCR 101 SSDFMSQLDL TKRPTSASLP PKRKGAIIID SRTAAVAACS HSGETISSCP 151 AMASITKPNS PPCARYKAVL RLSPTECFIL KAMANTTSAF NTSSIANSIN 201 TCRQPPINA* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m565/g565 100.0% identity in 67 aa overlap 10 20 30 40 50 60 m565.pep MDSTLSKTCCVSCILLSVTTTIFARPRPAASΝTSLRFASPΝDTGSPALLATCTRAMSKSS II II II II I II II I I II II II I I II II II II II I II II II II I I II I I II II II I II I II g565 MDSTLSKTCCVSCILLSVTTTIFARPRPAASΝTSLRFASPΝDTGSPALLATCTRAMSKSS 10 20 30 40 50 60 70 80 90 100 110 120 m565. pep AKYGISSWARTRPTVCPPLPKPTISTWSDLLMVSTSILCRSSDFMSQLDLTKRPTSASLP g565 AKYGISSLGEDASDRLPAPAEADΝQHMIRLAHRFHQHAVQIERLHVPAX The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1675>: a565. seq 1 ATGGACAGCA CATTGTCTAA AACGTGTTGC GTTTCGTGCA TATTGTTGAG 51 CGTAACCACC ACCATTTTCG CCCGTCCCAG ACCGGCGGCT TCCAATACTT 101 CCCTGCGTTT CGCATCGCCG AACGACACCG GTTCGCCCGC ACTTCTGGCA 151 ACCTGCACCC GCGCAATGTC CAAGTCGAGC GCGAAATACG GAATATCCTC 201 TTGGGCAAGG ACGCGTCCGA CCGTCTGCCC GCCCCTGCCG AAGCCGACAA 251 TCAGCACATG GTCGGACTTG CTCATGGTTT CTACCAGCAT ACTGTGCAGA 301 TCGAGCGACT TCATGTCCCA GCTTGACTTG ACCAAACGCC CGACCAGTGC 351 ATCGCTGCCG CCCAAGAGGA AGGGCGCGAT AATCATCGAC AGCAGAACCG 401 CCGCCGTCGC CGCCTGTTCC CATTCTAGCG AAACCATATC AAGCTGCCCG 451 GCAATGGCCA GCATCACGAA GCCGAACTCG CCGCCCTGCG CGAGATACAA 501 AGCCGTTTTG AGGCTGTCGC CGACCGAATG TTTCATTTTG AAGGCAATGG 551 CAAACACAAC CAGTGCCTTC AACACCAGCA GCATTGCCAA CAGCATCAAT 601 ACCTGCCGCC AGCCGCCGAT TAATGCCTGA This corresponds to the amino acid sequence <SEQ ID 1676; ORF 565. a>: a565.pep 1 MDSTLSKTCC VSCILLSVTT TIFARPRPAA SNTSLRFASP NDTGSPALLA 51 TCTRAMSKSS AKYGISSWAR TRPTVCPPLP KPTISTWSDL LMVSTSILCR 101 SSDFMSQLDL TKRPTSASLP PKRKGAIIID SRTAAVAACS HSSETISSCP 151 AMASITKPNS PPCARYKAVL RLSPTECFIL KAMANTTSAF NTSSIANSIN 201 TCRQPPINA* m565/a565 99.5% identity in 209 aa overlap 10 20 30 40 50 60 m565.pep MDSTLSKTCCVSCILLSVTTTIFARPRPAASNTSLRFASPNDTGSPALLATCTRAMSKSS II II I I II II II II II I I II I I II I I II II I I I I I II I I I II I I II II ll li I I I I i I i i a565 MDSTLSKTCCVSCILLSVTTTIFARPRPAASNTSLRFASPNDTGSPALLATCTRAMSKSS 10 20 30 40 50 60 70 80 90 100 110 120 m565.pep AKYGISSWARTRPTVCPPLPKPTISTWSDLLMVSTSILCRSSDFMSQLDLTKRPTSASLP II II II II II II II II II II II II II II II I I II I II II II II II II I II II II I I I I I I a565 AKYGISSWARTRPTVCPPLPKPTISTWSDLLMVSTSILCRSSDFMSQLDLTKRPTSASLP 70 80 90 100 110 120 130 140 150 160 170 180 m565.pep PKRKGAIIIDSRTAAVAACSHSGETISSCPAMASITKPNSPPCARYKAVLRLSPTECFIL I II II II II II I II II II II II : II II II I I II II II II II II II II II I I I II I I I I I I a565 PKRKGAIIIDSRTAAVAACSHSSETISSCPAMASITKPNSPPCARYKAVLRLSPTECFIL 130 140 150 160 170 180 190 200 210 m565.pep KAMANTTSAFNTSSIANSINTCRQPPINAX II I II II II I II I II II II II I II I II II I a565 KAMANTTSAFNTSSIANSINTCRQPPINAX 190 200 210 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1677>: g566. seq.. 1 atgccgtctg aacaatatct tttcagacgg cattttgtat gggggttaac 51 ggttgttcag cccgagtacg tcctgcatat cgtacaaacc cgttttgccg 101 tttacccaaa ctgcggcgcg gacggcaccg gcggcaaagg tcatgcggct 151 gccggctttg tgggtgattt ccacgcgttc gccgtcggtg gcgaagaggg 201 cggtgtggtc gccgactatg tcgcctgcgc ggacggtggc aaagccgatg 251 gtggaaggat cgcgcggacc agtgtggcct tcgcggccgt aaacggcgca 301 ttgtttgagg tcgcggccga gcgcgccggc gatgacttcg cccattcgta 351 a This corresponds to the amino acid sequence <SEQ ID 1678; ORF 566.ng>: g566.pep.. 1 MPSEQYLFRR HFVWGLTWQ PEYVLHIVQT RFAVYPΝCGA DGTGGKGHAA 51 AGFVGDFHAF AVGGEEGGW ADYVACADGG KADGGRIART SVAFAAVΝGA 101 LFEVAAERAG DDFAHS* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1679>: m566. seq.. 1 ATGCCGTCTG AACAATATCT TTTCAGACGG CATTTTGTAT GGGGGTTAAC 51 GGTTGTTCAG CCCGAGTACG TCCTGCATAT CGTACAAACC CGTTTTGCCG 101 TTGACCCAAA CTGCGGCGCG GACGGCACCG GCGGCAAAGG TCATGCGGCT 151 GCTGGCCTTG TGGGTGATTT CCACGCGCTC GCCGTCGGTG GCGAAGAGGG 201 CGGTGTGGTC GCCGACGATG TCGCCTGCGC GGACGGTGGC AAAGCCGATG 251 GTCGACGGAT CGCGCGGACC GGTGTGGCCT TCGCGGCCGT AAACGGCGCA 301 TTGTTTGAGG TCTCTGCCGA GCGCGCCGGC GATGACTTCG CCCATGCGTA 351 A This corresponds to the amino acid sequence <SEQ ID 1680; ORF 566>: m566.pep.. 1 MPSEQYLFRR HFVWGLTWQ PEYVLHIVQT RFAVDPΝCGA DGTGGKGHAA 51 AGLVGDFHAL AVGGEEGGW ADDVACADGG KADGRRIART GVAFAAVΝGA 101 LFEVSAERAG DDFAHA* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m566/g566 93.1% identity in 116 aa overlap 10 20 30 40 50 60 m566. pep MPSEQYLFRRHFVWGLTWQPEYVLHIVQTRFAVDPΝCGADGTGGKGHAAAGLVGDFHAL I I I I II II II II I II I I I I I II I I II I I I I I I II II II II II I I I II II II : II I I I I : g566 MPSEQYLFRRHFVWGLTWQPEYVLHIVQTRFAVYPNCGADGTGGKGHAAAGFVGDFHAF 10 20 30 40 50 60 70 80 90 100 110 m566. pep AVGGEEGGWADDVACADGGKADGRRIARTGVAFAAVNGALFEVSAERAGDDFAHAX II I II II II II I II II II II I II II II I I II I I I I II II I : I II I I II II I : I g566 AVGGEEGGWADYVACADGGKADGGRIARTSVAFAAVNGALFEVAAERAGDDFAHSX 70 80 90 100 110 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1681>: a566. seq 1 ATGCCGTCTG AACAATATCT TTTCAGACGG CATTTTGTAT GGGGGTTAAC 51 GGTTGTTCAG CCCGAGTACG TCCTGCATAT CGTACAAACC CGTTTTACCG 101 TTTACCCAAA CTGCGGCGCG GACGGCGCCG GCGGCAAAGG TCATGCGGCT 151 GCTTGCCTTG TGGGTGATTT CCACGCGCTC GCCGTCGGTG GCGAAGAGGG 201 CGGTGTGGTC GCCGACGATG TCGCCCGCGC GGACGGTGGC AAAGCCGATG 251 GTGGACGGAT CGCGCGGGCC GGTGTGGCCT TCGCGGCCGT AAACGGCGCA 301 TTGTTTGAGG TCTCTGCCGA GCGCGCCGGC GATGACTTCG CCCATGCGTA 351 A This corresponds to the amino acid sequence <SEQ ID 1682; ORF 566. a>: a566.pep 1 MPSEQYLFRR HFVWGLTWQ PEYVLHIVQT RFTVYPΝCGA DGAGGKGHAA 51 ACLVGDFHAL AVGGEEGGW ADDVARADGG KADGGRIARA GVAFAAVΝGA 101 LFEVSAERAG DDFAHA* m566/a566 94.0% identity in 116 aa overlap 10 20 30 40 50 60 m566. pep MPSEQYLFRRHFVWGLTWQPEYVLHIVQTRFAVDPΝCGADGTGGKGHAAAGLVGDFHAL I I I II I II II II II II II II I II II II II II I : I II II II I M I II II I I llllllll a566 MPSEQYLFRRHFVWGLTWQPEYVLHIVQTRFTVYPΝCGADGAGGKGHAAACLVGDFHAL 10 20 30 40 50 60 70 80 90 100 110 m566. pep AVGGEEGGWADDVACADGGKADGRRIARTGVAFAAVΝGALFEVSAERAGDDFAHAX lllllllllllllll I II II II I II I I : II I II II II II I II II II II I I II I I I a566 AVGGEEGGWADDVARADGGKADGGRIARAGVAFAAVΝGALFEVSAERAGDDFAHAX 70 80 90 100 110 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 1683>: g56 . seq.. 1 atgcgacgac gggcagcggc atcgacaagg cgggtttgca gtccggcgtt 51 tatcaggtct tattgggcga tgcggacgtg cagtcggcgg cggtacgcag 101 caaagagggc ggatacggcg tgttgggtgc gaacgcgcgc gcttgccggc 151 gcggaaatcg agctggtgca ggaaatcgcc cgggaagtgc gtttgaaaaa 201 cgcgctcaag gcagtggcgg aagattacga ctttatcctg atcgactgtc 251 cgccttcgct gacgctgttg acgcttaacg gcttggtggc ggcgggcggc 301 gtgattgtgc cgatgttgtg cgaatattac gcgctggaag ggatttccga 351 tttgattgcg accgtgcgca aaatccgtca ggcggtcaat cccgatttgg 401 acatcacggg catcgtgcgt acgatgtacg acagccgcag caggctggtt 451 gccgaagtca gcgaacagtt gcgcagccat ttcggggatt tgctttttga 501 aaccgccatc ccgcgcaata tccgccttgc ggaagcgccg agccacggta 551 tgccggtgat ggcttacgac gcgcaggcaa agggtgccaa ggcgtatctt 601 gccttggcgg acgaactggc ggcgagggtg tcggggaaat ag This corresponds to the amino acid sequence <SEQ ID 1684; ORF 567.ng>: g567.pep 1 MRRRAAASTR RVCSPAFIRS YWAMRTCSRR RYAAKRADTA CWVRTRALAG 51 AEIELVQEIA REVRLKΝALK AVAEDYDFIL IDCPPSLTLL TLΝGLVAAGG 101 VIVPMLCEYY ALEGISDLIA TVRKIRQAVΝ PDLDITGIVR TMYDSRSRLV 151 AEVSEQLRSH FGDLLFETAI PRΝIRLAEAP SHGMPVMAYD AQAKGAKAYL 201 ALADELAARV SGK* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1685>: ιtι567. seq.. 1 ATGAGTGCGA ACATCCTTGC CATCGCCAAT CAGAAGGGCG GTGTGGGCAA 51 AACGACGACG ACGGTAAATT TGGCGGCTTC GCTGGCATCG CGCGGCAAAC 101 GCGTGCTGGT GGTCGATTTG GATCCGCAGG GCAATGCGAC GACGGGCAGC 151 GGCATCGACA AGGCGGGTTT GCAGTCCGGC GTTTATCAGG TCTTATTGGG 201 CGATGCGGAC GTGCAGTCGG CGGCGGTACG CAGCAAAGAG GGCGGATACG 251 CTGTGTTGGG TGCGAACCGC GCGCTGGCCG GCGCGGAAAT CGAACTGGTG 301 CAGGAAATCG CCCGGGAAGT GCGTTTGAAA AACGCGCTCA AGGCAGTGGA 351 AGAAGATTAC GACTTTATCC TGATCGACTG CCCGCCTTCG CTGACGCTGT 401 TGACGCTTAA CGGGCTGGTG GCGGCGGGCG GCGTGATTGT GCCGATGTTG 451 TGCGAATATT ACGCGCTGGA AGGGATTTCC GATTTGATTG CGACCGTGCG 501 CAAAATCCGT CAGGCGGTCA ATCCCGATTT GGACATCACG GGCATCGTGC 551 GCACGATGTA CGACAGCCGC AGCAGGCTGG TTGCCGAAGT CAGCGAACAG 601 TTGCGCAGCC ATTTCGGGGA TTTGCTTTTT GAAACCGTCA TCCCGCGCAA 651 TATCCGCCTT GCGGAAGCGC CGAGCCACGG TATGCCGGTG ATGGCTTACG 701 ACGCGCAGGC AAAGGGTACC AAGGCGTATC TTGCCTTGGC GGACGAGCTG 751 GCGGCGAGGG TGTCGGGGAA ATAG This corresponds to the amino acid sequence <SEQ ID 1686; ORF 567>: m567.pep.. 1 MSAΝILAIAΝ QKGGVGKTTT TVΝLAASLAS RGKRVLWDL DPQGΝATTGS 51 GIDKAGLQSG VYQVLLGDAD VQSAAVRSKE GGYAVLGAΝR ALAGAEIELV 101 QEIAREVRLK ΝALKAVEEDY DFILIDCPPS LTLLTLΝGLV AAGGVIVPML 151 CEYYALEGIS DLIATVRKIR QAVΝPDLDIT GIVRTMYDSR SRLVAEVSEQ 201 LRSHFGDLLF ETVIPRΝIRL AEAPSHGMPV MAYDAQAKGT KAYLALADEL 251 AARVSGK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m567/g567 98.2% identity in 168 aa overlap 60 70 80 90 100 110 119 m567. pep GVYQVLLGDADVQSAAVRSKEGGYAVLGAΝRALAGAEIELVQEIAREVRLKΝALKAVEED II I II II II II I II I II I II II II II I II g567 AFIRSYWAMRTCSRRRYAAKRADTACWVRTRALAGAEIELVQEIAREVRLKΝALKAVAED 20 30 40 50 60 70 120 130 140 150 160 170 179 m567. pep YDFILIDCPPSLTLLTLΝGLVAAGGVIVPMLCEYYALEGISDLIATVRKIRQAVΝPDLDI II I II II II II II I II II II II II II II I I II I II II I II II II II II II II II II II II g567 YDFILIDCPPSLTLLTLΝGLVAAGGVIVPMLCEYYALEGISDLIATVRKIRQAVΝPDLDI 80 90 100 110 120 130 180 190 200 210 220 230 239 m567. pep TGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRΝIRLAEAPSHGMPVMAYDAQAKG II I II II II II II I II II II II I II I II I II I I: II I II II II I I II I II I I I I II I I I I g567 TGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETAIPRΝIRLAEAPSHGMPVMAYDAQAKG 140 150 160 170 180 190 240 250 m567. pep TKAYLALADELAARVSGKX : II I II I II II II II II II g567 AKAYLALADELAARVSGKX 200 210 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1687>: a567. seq 1 ATGAGTGCGA ACATCCTTGC CATCGCCAAT CAGAAGGGCG GTGTGGGCAA 51 AACGACGACG ACGGTAAATT TGGCGGCTTC GCTGGCATCG CGCGGCAAAC 101 GCGTGCTGGT GGTCGATTTG GATCCGCAGG GCAATGCGAC GACGGGCAGC 151 GGCATCGACA AGGCGAGTTT GCAGTCCGGC GTTTATCAGG TCTTATTGGG 201 CGATGCGGAC GTGAAATCGG CGGCGGTACG CAGCAAAGAG GGCGGATACG 251 GCGTGTTGGG TGCGAACCGC GCGCTGGCCG GCGCGGAAAT CGAGCTGGTG 301 CAGGAAATCG CCCGGGAAGT GCGTTTGAAA AACGCGCTCA AGGCAGTGGC 351 GGAAGATTAC GACTTTATCC TGATCGACTG CCCGCCTTCG CTGACGCTGT 401 TGACGCTTAA CGGCTTGGTG GCGGCAGGCG GCGTGATTGT GCCGATGTTG 451 TGCGAATATT ACGCGCTGGA AGGGATTTCC GATTTGATTG CGACCGTGCG 501 CAAAATCCGT CAGGCGGTCA ATCCCGATTT GGATATCACG GGCATCGTGC 551 GTACGATGTA CGACAGCCGC AGCAGGCTAG TTGCCGAAGT CAGCGAACAG 601 TTGCGCAGCC ATTTCGGGGA TTTGCTGTTT GAAACCGTCA TCCCGCGCAA 651 TATCCGCCTT GCGGAAGCGC CGAGCCACGG TATGCCGGTG ATGGCTTATG 701 ATGCGCAGGC AAAGGGTGCC AAGGCGTATC TTGCCTTGGC GGACGAGCTG 751 ATGGCGAGGG TGTCGGGGAA ATAG This corresponds to the amino acid sequence <SEQ ID 1688; ORF 567. a>: a567.pep 1 MSANILAIAN QKGGVGKTTT TVNLAASLAS RGKRVLWDL DPQGNATTGS 51 GIDKASLQSG VYQVLLGDAD VKSAAVRSKE GGYGVLGANR ALAGAEIELV 101 QEIAREVRLK NALKAVAEDY DFILIDCPPS LTLLTLNGLV AAGGVIVPML 151 CEYYALEGIS DLIATVRKIR QAVNPDLDIT GIVRTMYDSR SRLVAEVSEQ 201 LRSHFGDLLF ETVIPRNIRL AEAPSHGMPV MAYDAQAKGA KAYLALADEL 251 MARVSGK* m567/a567 97.7% identity in 257 aa overlap 10 20 30 40 50 60 m567 ■pep MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLWDLDPQGNATTGSGIDKAGLQSG I I I I I II II II I II II II I II II II II II II II II I II II II II II II II II I II : I I I I a567 MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLWDLDPQGNATTGSGIDKASLQSG 10 20 30 40 50 60 70 80 90 100 110 120 m567. pep VYQVLLGDADVQSAAVRSKEGGYAVLGANRALAGAEIELVQEIAREVRLKNALKAVEEDY II I II II II II M II II II II II: II I II II I II II II II I II I II II II II I II I III a567 VYQVLLGDADVKSAAVRSKEGGYGVLGANRALAGAEIELVQEIAREVRLKNALKAVAEDY 70 80 90 100 110 120 130 140 150 160 170 180 m567.pep DFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGISDLIATVRKIRQAVNPDLDIT I I I I II I II I II II II II I II I II II II II I II II II II I II II II I I II II II II II II a567 DFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGISDLIATVRKIRQAVNPDLDIT 130 140 150 160 170 180 190 200 210 220 230 240 m567.pep GIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRNIRLAEAPSHGMPVMAYDAQAKGT I I I I II II II I I II II I II II I II II II II II I II I II II II II II I I II I I I I I I II I : a567 GIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRNIRLAEAPSHGMPVMAYDAQAKGA 190 200 210 220 230 240 250 m567.pep KAYLALADELAARVSGKX II I I II II II lllllll a567 KAYLALADELMARVSGKX 250 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1689>: g568. seq 1 atgctcaggg tcagaccggt attatttgcc gtcaaggctt ccgcctcttc 51 gataccttgc agaatctgcc gattaaagcg ttcgcggctg cccaatattt 101 tcaggcgcat attgttttcg tgcaggcggc gtacctgttt ttgcaaagcc 151 tgtaaaaaca gccccatcag gaacgaaact tcgtcttcgg ggcgacgcca 201 gttttcggtt gaaaaggcaa acacggtcag atattgcacg cccagtttgg 251 cgcaatgctt caccatattt tccaacgcgt ccaagccgcg tttgtgtccc 301 attatacgcg ggagaaaacg ttttttcgcc caacggccgt tgccgtccat 351 aattacggcg atgtgcctcg ggatggcggt gtgttccaaa atggtctgcg 401 tgctgctctt catatctgcc tttcgcggtt cggcgttcaa atgccgtctg 451 aacgccgcgc cgtga This corresponds to the amino acid sequence <SEQ ID 1690; ORF 568.ng>: g568.pep 1 MLRVRPVLFA VKASASSIPC RICRLKRSRL PNIFRRILFS CRRRTCFCKA 51 CKNSPIRNET SSSGRRQFSV EKANTVRYCT PSLAQCFTIF SNASKPRLCP 101 IIRGRKRFFA QRPLPSIITA MCLGMAVCSK MVCVLLFISA FRGSAFKCRL 151 NAAP* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1691>: m568. seq 1 ATGCTCAGGG TCAGGCCGGT ATTGTTTGCC GTCAACGCTT CCGCCTCTTC 51 GATGCCTTGC AGAATCTGCC GGTTGAAGCG TTCGCGGCTG CCCAATATCT 101 TCAGGCGCAT ATTGTTTTCG TGCAGGCGGC GTACCTGTTT TTGCAAAGCC 151 TGTAAAAACA GCCCCATCAG GAACGAAACT TCGTCTTCGG GGCGGCGCCA 201 GTTTTCGGTT GAAAAGGCAA ACACGGTCAG ATATTGCACA CCCAGTTTGG 251 CGCAATGCTT CACCATATTT TCCAATGCGT CCAAACCGCG TTTGTGTCCC 301 ATTATGCGCG GGAGGAAACG TTTTTTCGCC CAACGGCCGT TGCCGTCCAT 351 AATCACGGCG ATATGCTTGG GAATGGCGGT GTGTTCCAAA ACGGCCTGCG 401 TGCTGCTTTT CATGTCTGCC TTTCGCGGTT CGGCATTCAA ATGCCGTCTG 451 AACGCCGAAC CGTGCAGGTT AAATTGCCAT CAAATCTTCT TCTTTGGCAG 501 TCAGGAGTTT GTCGGCTTCG GTAATGTATT TGTCGGTCAG TTTTTGAACC 551 GCTTCTTCGC CGCGACGTGC CTCGTCTTCG GAAATTTCTT TGTCTTTGAG 601 GAGTTTTTTG ATGTGGTCGT TGGCATCGCG GCGCACGTTG CGGATAGAGA 651 CGCGGCCTTC TTCCGCTTCG CCGCGTACGA CTTTAATCAG GTCTTTGCGG 701 CGTTCCTCGG TCAGCATGGG CATCGGCACG CGGATCAGGT CGCCGACAGC 751 TGCCGGGTTC AGTCCCAAGT TTGA This corresponds to the amino acid sequence <SEQ ID 1692; ORF 568>: m568.pep.. 1 MLRVRPVLFA VΝASASSMPC RICRLKRSRL PNIFRRILFS CRRRTCFCKA 51 CKNSPIRNET SSSGRRQFSV EKANTVRYCT PSLAQCFTIF SNASKPRLCP 101 IMRGRKRFFA QRPLPSIITA ICLGMAVCSK TACVLLFMSA FRGSAFKCRL 151 NAEPCRLNCH QIFFFGSQEF VGFGNVFVGQ FLNRFFAATC LVFGNFFVFE 201 EFFDVWGIA AHVADRDAAF FRFAAYDFNQ VFAAFLGQHG HRHADQVADS 251 CRVQSQV* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m568/g568 94.8% identity in 154 aa overlap 10 20 30 40 50 60 m568. pep MLRVRPVLFAVΝASASSMPCRICRLKRSRLPΝIFRRILFSCRRRTCFCKACKΝSPIRΝET I II I I II I II I : I II II M II II I I II I I I II I I II II I II I I I I II I II II II II I II I g568 MLRVRPVLFAVKASASSIPCRICRLKRSRLPΝIFRRILFSCRRRTCFCKACKΝSPIRΝET 10 20 30 40 50 60 70 80 90 100 110 120 m568.pep SSSGRRQFSVEKAΝTVRYCTPSLAQCFTIFSΝASKPRLCPIMRGRKRFFAQRPLPSIITA MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g568 SSSGRRQFSVEKAΝTVRYCTPSLAQCFTIFSΝASKPRLCPIIRGRKRFFAQRPLPSIITA 70 80 90 100 . 110 120 130 140 150 160 170 180 m568.pep ICLGMAVCSKTACVLLFMSAFRGSAFKCRLΝAEPCRLΝCHQIFFFGSQEFVGFGΝVFVGQ : I II I II II I M II II: II II II II II II II I g568 MCLGMAVCSKMVCVLLFISAFRGSAFKCRLΝAAPX 130 140 150 190 200 210 220 230 240 m568. pep FLΝRFFAATCLVFGΝFFVFEEFFDWVGIAAHVADRDAAFFRFAAYDFΝQVFAAFLGQHG The following partial DNA sequence was identified in N meningitidis <SEQ ID 1693>: a568. seq 1 ATGCTCAGGG TCAGGCCGGT ATTGTTTGCC GTCAAGGCTT CCGCCTCTTC 51 GATGCCCTTC AGGATTTGAC GGTTGAAGCG TTCGCGGCTG CCCAGTATTT 101 TCAGGCGCAT ATTGTTTTCG TGCAGGCGGC GTACCTGTTT TTGCAAAGCC 151 TGTAAAAACA GCCCCATCAG GAACGAAACT TCGTCTTCGG GGCGGCGCCA 201 GTTTTCGGTT GAAAAGGCAA ACACGGTCAG ATATTGCACA CCCAGTTTGG 251 CGCAATGCTT CACCATATTT TCCAATGCGT CCAAACCGCG TTTGTGTCCC 301 ATTATGCGCG GGAGGAAACG TTTTTTCGCC CAACGGCCGT TGCCGTCCAT 351 AATCACGGCG ATATGCTTGG GAATGGCGGT GTGTTCCAAA ACGGCCTGCG 401 TGCTGCTTTT CATGTCTGCC TTTCGCGGTT CGGCATTCAA ATGCCGTCTG 451 AACGCCGAAC CGTGCAGGTT AAATTGCCAT CAAATCTTCT TCTTTGGCAG 501 TCAGGAGTTT GTCGGCTTCG GTAATGTATT TGTCGGTCAG TTTTTGAACC 551 GCTTCTTCGC CGCGACGTGC CTCGTCTTCG GAAATTTCTT TGTCTTTGAG 601 GAGTTTTTTG ATGTGGTCGT TGGCATCGCG GCGCACGTTG CGGATGGAGA 651 CGCGGCCTTC TTCCGCTTCG CCGCGTACGA CTTTAATCAG GTCTTTGCGG 701 CGTTCCTCGG TCAGCATGGG CATCGGCACG CGGATCAGGT CGCCGACAGC 751 TGCCGGGTTC AGTCCCAAGT TTGA This corresponds to the amino acid sequence <SEQ ID 1694; ORF 568. a>: a568.pep 1 MLRVRPVLFA VKASASSMPF RI*RLKRSRL PSIFRRILFS CRRRTCFCKA 51 CKNSPIRNET SSSGRRQFSV EKANTVRYCT PSLAQCFTIF SNASKPRLCP 101 IMRGRKRFFA QRPLPSIITA ICLGMAVCSK TACVLLFMSA FRGSAFKCRL 151 NAEPCRLNCH QIFFFGSQEF VGFGNVFVGQ FLNRFFAATC LVFGNFFVFE 201 EFFDVWGIA AHVADGDAAF FRFAAYDFNQ VFAAFLGQHG HRHADQVADS 251 CRVQSQV* m568/a568 98.1% identity in 257 aa overlap 10 20 30 40 50 60 m568. pep MLRVRPVLFAVNASASSMPCRICRLKRSRLPNIFRRILFSCRRRTCFCKACKNSPIRNET II I II II II I I : II I II II II II II I II I : I II II II II II II II I II II II II II I I a568 MLRVRPVLFAVKASASSMPFRIXRLKRSRLPSIFRRILFSCRRRTCFCKACKNSPIRNET 10 20 30 40 50 60 70 80 90 100 110 120 m568.pep SSSGRRQFSVEKANTVRYCTPSLAQCFTIFSNASKPRLCPIMRGRKRFFAQRPLPSIITA II II II I II II II I II I I II I I II II II II II II II II II II II II II II I II I I I I I II a568 SSSGRRQFSVEKANTVRYCTPSLAQCFTIFSNASKPRLCPIMRGRKRFFAQRPLPSIITA 70 80 90 100 110 120 130 140 150 160 170 180 m568. pep ICLGMAVCSKTACVLLFMSAFRGSAFKCRLNAEPCRLNCHQIFFFGSQEFVGFGNVFVGQ MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a568 ICLGMAVCSKTACVLLFMSAFRGSAFKCRLNAEPCRLNCHQIFFFGSQEFVGFGNVFVGQ 130 140 150 160 170 180 190 200 210 220 230 240 m568. pep FLNRFFAATCLVFGNFFVFEEFFDVWGIAAHVADRDAAFFRFAAYDFNQVFAAFLGQHG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M M M M M M M M M M M M a568 FLNRFFAATCLVFGNFFVFEEFFDVWGIAAHVADGDAAFFRFAAYDFNQVFAAFLGQHG 190 200 210 220 230 240 250 m568.pep HRHADQVADSCRVQSQVX II II I II II II II I II II a568 HRHADQVADSCRVQSQVX 250 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1695>: g569.seq.. 1 atgctgaaac aacgggtaat aaccgctatg tggctgctgc cgctgatgct 51 gggcatgctg ttttacgcgc cgcaatggct gtgggctgca ttttgcgggc 101 tgattgccct gaccgccttg tgggagtatg cccgtatggc cggtttgtgc 151 aaaaccgaaa ccaaccatta cctcgccgca accttggttt tcggcgtagt 201 tgcctatgcg ggcggctgga tgctgcctaa tttggtttgg tatgttgttt 251 tggcattttg gctcgccgtt atgcctttgt ggttgagatt caaatggagg 301 ctcaacggcg gttggcaggt ttatgccgtc ggctggcttt tgctcatgcc 351 gttttggttc gcgctcgtat ccctggcgcc cgcatcccga tga This corresponds to the amino acid sequence <SEQ ID 1696; ORF 569.ng>: g569.pep 1 MLKQRVITAM WLLPLMLGML FYAPQWLWAA FCGLIALTAL WEYARMAGLC 51 KTETNHYLAA TLVFGWAYA GGW LPNLVW YWLAFWLAV MPLWLRFKWR 101 LNGGWQVYAV GWLLLMPFWF ALVSLAPASR * The following partial DNA sequence was identified in N meningitidis <SEQ ID 1697>: m569.seq.. 1 ATGCTGAAAC AACGGGTAAT AACCGCCATG TGGCTGCTGC CGCTGATGCT 51 GGGCATGCTG TTTTACGCGC CGCAATGGTT GTGGGCTGCA TTTTGCGGAC 101 TGATTGCCCT GATTGCCTTG TGGGAATATG CCCGTATGGG CGGTTTGTGC 151 AAAATTAAAA CCAACCATTA CCTCGCCGCA ACCTTGGTTT TCGGCGTGGT 201 TGCCTATGCG GGCGGCTGGA TGCTGCCTAA TTTGGTTTGG TATGTTGTTT 251 TGGCATTTTG GCTCGCCGTT ATGCCTTTAT GGTTGAGATT CAAATGGAGG 301 CTCAACGGCG GTTGGCAGGT TTATGCCGTC GGCTGGCTTC TGGTCATGCC 351 GTTTTGGTTC GCGCTCGTAT CCCTGCGCCC GCATCCCGAT GATGCCCTGC 401 CGCTGCTCGC CGTGATGGGT TTGGTGTGGG TTGCCGATAT TTGCGCGTAT 451 TTCAGCGGCA AGGCGTTCGG CAAACACAAA ATCGCGCCGG CAATCAGCCC 501 CGGCAAAAGC TGGGAAGGTG CAATCGGCGG CGCGGTTTGC GTGGCAGTGT 551 ACATGACCGC CGTACGAAGT GCCGGCTGGC TGGCATTCGA TACAGGCTGG 601 TTCGATACCG TGTTAATCGG TTTGGTGCTG ACCGTTGTCA GCGTATGCGG 651 CGACCTTTTG GAAAGCTGGC TCAAGCGCGC GGCAGGCATC AAAGACAGCA 701 GCAAGCTGCT GCCCGGACAC GGCGGCGTGT TCGACCGTAC CGACAGCCTG 751 ATTGCCGTTA TCAGCGTCTA TGCAGCGATG ATGTCGGTTT TAAATTGA This corresponds to the amino acid sequence <SEQ ID 1698; ORF 569>: m569.pep.. 1 MLKQRVITAM WLLPLMLGML FYAPQWLWAA FCGLIALIAL WEYARMGGLC 51 KIKTΝHYLAA TLVFGWAYA GGWMLPNLVW YWLAFWLAV MPLWLRFKWR 101 LNGGWQVYAV GWLLVMPFWF ALVSLRPHPD DALPLLAVMG LVWVADICAY 151 FSGKAFGKHK IAPAISPGKS WEGAIGGAVC VAVYMTAVRS AGWLAFDTGW 201 FDTVLIGLVL TWSVCGDLL EΞWLKRAAGI KDSSKLLPGH GGVFDRTDSL 251 IAVISVYAAM MSVLN* m569/g569 95.3% identity in 127 aa overlap 10 20 30 40 50 60 m569.pep MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYARMGGLCKIKTNHYLAA M M M M I N I M g569 MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALTALWEYARMAGLCKTETNHYLAA 10 20 30 40 50 60 70 80 90 100 110 120 m569. pep TLVFGWAYAGGWMLPNLVWYWLAFWLAVMPLWLRFKWRLNGGWQVYAVGWLLVMPFWF g569 TLVFGWAYAGGWMLPNLVWYWLAFWLAVMPLWLRFKWRLNGGWQVYAVGWLLLMPFWF 70 80 90 100 110 120 130 140 150 160 170 180 m569 . pep ALVSLRPHPDDALPLLAVMGLVWVADICAYFSGKAFGKHKIAPAISPGKΞWEGAIGGAVC M i l l I g569 ALVSLAPASRX 130 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1699>: a569. seq 1 ATGCTGAAAC AACGGGTGAT AACCGCCATG TGGCTGCTGC CGCTGATGCT 51 GGGCATGCTG TTTTACGCGC CGCAATGGTT GTGGGCTGCA TTTTGCGGAC 101 TGATTGCCCT GATTGCCTTG TGGGAATATG CCCGTATGGG CGGTTTGTGC 151 7AAAATTAAAA CCAACCATTA CCTCGCCGCA ACCTTGGTTT TCGGCGTGGT 201 TGCCTATGCG GGCGGCTGGA TGCTGCCTAA TTTGGTTTGG TATGTTGTTT 251 TGGCATTTTG GCTCGCCGTT ATGCCTTTAT GGTTGAGATT CAAATGGAGG 301 CTCAACGGCG GTTGGCAGGT TTATGCCGTC GGCTGGCTTC TGGTCATGCC 351 GTTTTGGTTC GCGCTCGTAT CCCTGCGCCC GCATCCCGAT GATGCCCTGC 401 CGCTGCTCGC CGTGATGGGT TTGGTGTGGG TTGCCGATAT TTGCGCGTAT 451 TTCAGCGGCA AGGCGTTCGG CAAACACAAA ATCGCACCGG CAATCAGCCC 501 CGGCAAAAGC TGGGAAGGTG CAATCGGCGG CGCGGTTTGC GTGGCCGTGT 551 ACATGACCGC CGTACGAAGT GCCGGCTGGC TGGCATTCGA TACAGGCTGG 601 TTCGATACCG TGTTAATCGG TTTGGTGTTG ACCGTTGTCA GCGTATGCGG 651 CGACCTTTTG GAAAGCTGGC TCAAGCGCGC GGCAGGCATC AAAGACAGCA 701 GCAACCTGCT GCCCGGACAC GGCGGCGTGT TCGACCGCAC CGACAGCCTG 751 ATTGCCGTTA TCAGCGTCTA TGCAGCGATG ATGTCGGTTT TAAATTGA This corresponds to the amino acid sequence <SEQ ID 1700; ORF 569. a>: a569.pep 1 MLKQRVITAM WLLPLMLGML FYAPQWLWAA FCGLIALIAL WEYARMGGLC 51 KIKTNHYLAA TLVFGWAYA GGWMLPNLVW YWLAFWLAV MPLWLRFKWR 101 LNGGWQVYAV GWLLVMPFWF ALVSLRPHPD DALPLLAVMG LVWVADICAY 151 FSGKAFGKHK IAPAISPGKS WEGAIGGAVC VAVYMTAVRS AGWLAFDTGW 201 FDTVLIGLVL TWSVCGDLL ESWLKRAAGI KDSSNLLPGH GGVFDRTDSL 251 IAVISVYAAM MSVLN* m569/a569 99.6% identity in 265 aa overlap 10 20 30 40 50 60 m569. pep MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYARMGGLCKIKTNHYLAA I II I II II II II II II II II II II II II II II I I II II II I II I I I II II II II II I I I I a569 MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYARMGGLCKIKTNHYLAA 10 20 30 40 50 60 70 80 90 100 110 120 m569. pep TLVFGWAYAGGWMLPNLVWYWLAFWLAVMPLWLRFKWRLNGGWQVYAVGWLLVMPFWF II II II II II I II II II II I II II II II II I I I I I I I II II I II II II II II I II M M I a569 TLVFGWAYAGGWMLPNLVWYWLAFWLAVMPLWLRFKWRLNGGWQVYAVGWLLVMPFWF 70 80 90 100 110 120 130 140 150 160 170 180 m569.pep ALVSLRPHPDDALPLLAVMGLVWVADICAYFSGKAFGKHKIAPAISPGKSWEGAIGGAVC II II II II II II II I II II I II II II I II II I II II II I II I II II I II I II I I I I II II a569 ALVSLRPHPDDALPLLAVMGLVWVADICAYFSGKAFGKHKIAPAISPGKSWEGAIGGAVC 130 140 150 160 170 180 190 200 210 220 230 240 m569. pep VAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTWSVCGDLLESWLKRAAGIKDSSKLLPGH MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a569 VAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTWSVCGDLLESWLKRAAGIKDSSNLLPGH 190 200 210 220 230 240 250 260 m569.pep GGVFDRTDSLIAVISVYAAMMSVLNX II II II II II II II II II I I II II II a569 GGVFDRTDSLIAVISVYAAMMSVLNX 250 260 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1701>: g570.seq.. 1 atgatccgtt tgacccgcgc gtttgccgcc gccctgatcg gtttatgctg 51 caccacaggc gcgcacgccg acaccttcca aaaaatcggc tttatcaaca 101 ccgagcgcat ctacctcgaa tccaagcagg cgcgcaacat ccaaaaaacg 151 ctggacggcg aattttccgc ccgtcaggac gaattgcaaa aactgcaacg 201 cgaaggcttg gatttggaaa ggcagctcgc cggcggcaaa cttaaggacg 251 caaaaaaggc gcaagccgaa gaaaaatggc gcgggctggt cgaagcgttc 301 cgcaaaaaac aggcgcagtt tgaagaagac tacaacctcc gccgcaacga 351 agagtttgcc tccctccagc aaaacgccaa ccgcgtcatc gtcaaaatcg 401 ccaaacagga aggttacgat gtcattttgc aggacgtgat ttacgtcaac 451 acccaatacg acgttaccga cagcgtcatt aaagaaatga acgcccgctg 501 a This corresponds to the amino acid sequence <SEQ ID 1702; ORF 570.ng>: g570.pep.. 1 MIRLTRAFAA ALIGLCCTTG AHADTFQKIG FINTERIYLE SKQARNIQKT 51 LDGEFSARQD ELQKLQREGL DLERQLAGGK LKDAKKAQAE EKWRGLVEAF 101 RKKQAQFEED YNLRRNEEFA SLQQNANRVI VKIAKQEGYD VILQDVIYVN 151 TQYDVTDSVI KEMNAR* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1703>: m570.seq.. 1 ATGACCCGTT TGACCCGCGC GTTTGCCGCG GCTCTGATCG GTTTGTGCTG 51 CACCGCAGGC GCGCACGCCG ACACCTTCCA AAAAATCGGC TTTATCAACA 101 CCGAGCGCAT CTACCTCGAA TCCAAGCAGG CGCGCAAGAT TCAAAAAACG 151 CTGGACAGCG AATTTTCCGC TCGTCAGGAC GAATTGCAAA AACTGCAACG 201 CGAAGGTCTG GATTTGGAAA GGCAGCTTGC CGAAGGCAAA CTCAGAAACG 251 CAAAAAAGGC GCAAGCCGAA GAAAAATGGC GCGGGCTGGT CGCAGCGTTC 301 CGCAAAAAAC AGGCGCAGTT TGAAGAAGAC TACAACCTCC GCCGCAACGA 351 AGAGTTTGCC TCCCTCCAGC AAAACGCCAA CCGCGTCATC GTCAAAATCG 401 CCAAACAGGA AGGTTACGAT GTCATTTTGC AGAACGTGAT TTACGTCAAC 451 ACCCAATACG ACGTTACCGA CAGCGTCATT AAAGAAATGA ACGCCCGCTG 501 A This corresponds to the amino acid sequence <SEQ ID 1704; ORF 570>: m570.pep 1 MTRLTRAFAA ALIGLCCTAG AHADTFQKIG FINTERIYLE SKQARKIQKT 51 LDSEFSARQD ELQKLQREGL DLERQLAEGK LRNAKKAQAE EKWRGLVAAF 101 RKKQAQFEED YNLRRNEEFA SLQQNANRVI VKIAKQEGYD VILQNVIYVN 151 TQYDVTDSVI KEMNAR* m570/g570 94.6% identity in 166 aa overlap 10 20 30 40 50 60 m570.pep MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQARKIQKTLDSEFSARQD I g570 MIRLTRAFAAALIGLCCTTGAHADTFQKIGFINTERIYLESKQARNIQKTLDGEFSARQD 10 20 30 40 50 60 70 80 90 100 110 120 m570. pep ELQKLQREGLDLERQLAEGKLRNAKKAQAEEKWRGLVAAFRKKQAQFEEDYNLRRNEEFA I I M M I I M I M I I I I M I I 1 1 1 1 I I I I 1 1 1 1 1 1 1 1 1 1 1 1 g570 ELQKLQREGLDLERQLAGGKLKDAKKAQAEEKWRGLVEAFRKKQAQFEEDYNLRRNEEFA 70 80 90 100 110 120 130 140 150 160 m570.pep SLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVIKEMNARX g570 SLQQNANRVIVKIAKQEGYDVILQDVIYVNTQYDVTDSVIKEMNARX 130 140 150 160 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 1705>: a570. seq 1 ATGACCCGTT TGACCCGCGC GTTTGCCGCG GCTCTGATCG GTTTGTGCTG 51 CACCGCAGGC GCGCACGCCG ACACCTTCCA AAAAATCGGC TTTATCAACA 101 CCGAGCGCAT CTACCTCGAA TCCAAGCAGG CGCGCAAGAT TCAAAAAACG 151 CTGGACAGCG AATTTTCCGC CCGCCAGGAC GAATTGCAAA AACTGCAACG 201 CGAAGGTCTG GATTTGGAAA GGCAGCTTGC CGAAGGCAAA CTCAAAGACG 251 CAAAAAAGGC GCAAGCCGAA GAAAAATGGT GCGGGCTGGT CGCAGCGTTC 301 CGCAAAAAAC AGGCGCAGTT TGAAGAAGAC TACAACCTCC GCCGCAACGA 351 AGAGTTTGCC TCCCTCCAGC AAAACGCCAA CCGCGTCATC GTCAAAATCG 401 CCAAACAGGA AGGTTACGAT GTCATTTTGC AGGACGTGAT TTACGTCAAC 451 ACCCAATACG ACGTTACCGA CAGCGTCATT AAAGAAATGA ACGCCCGCTG 501 A This corresponds to the amino acid sequence <SEQ ID 1706; ORF 570. a>: a570.pep 1 MTRLTRAFAA ALIGLCCTAG AHADTFQKIG FINTERIYLE SKQARKIQKT 51 LDSEFSARQD ELQKLQREGL DLERQLAEGK LKDAKKAQAE EKWCGLVAAF 101 RKKQAQFEED YNLRRNEEFA SLQQNANRVI VKIAKQEGYD VILQDVIYVN 151 TQYDVTDSVI KEMNAR* m570/a570 97.6% identity in 166 aa overlap 10 20 30 40 50 60 m570.pep MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQARKIQKTLDSEFSARQD II II II II II II II II II II I I II II II II I II II II I II I II II I I II I II II II I I II a570 MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQARKIQKTLDSEFSARQD 10 20 30 40 50 60 70 80 90 100 110 120 m570. pep ELQKLQREGLDLERQLAEGKLRNAKKAQAEEKWRGLVAAFRKKQAQFEEDYNLRRNEEFA I II II II II II II II II II II :: I I II II II II II II II II II II II I II II II I II I I a570 ELQKLQREGLDLERQLAEGKLKDAKKAQAEEKWCGLVAAFRKKQAQFEEDYNLRRNEEFA 70 80 90 100 110 120 130 140 150 160 m570. pep SLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVIKEMNARX II II I II II II I I II II II II II I: II I II II II I I I I I II II I I II a570 SLQQNANRVIVKIAKQEGYDVILQDVIYVNTQYDVTDSVIKEMNARX 130 140 150 160 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1707>: g571.seq (partial) 1 atgcgcgttt tccgagtaaa ccgatttgtt gttaccgttt tcggcggcgg 51 tataggttct gccgtcccac acgctgcctg cgtcggcaaa caggctcagg 101 cggacggtgc gtgcgtcttt cgcaccgggc atcgggaaga gcagctcggc 151 ggagacgttg gcttttttgt tgccgccgta gctgattttt tcgccgtatt 201 cgtcatacac tttcgggccg agcgtgccgc tttcgtagcc gcgcaccgaa 251 cccaggccgc cgccgtagaa gttttcaaag aaggggattt ctttggttct 301 gccgtagccg cccgcaatgc cgacttcgcc gccgagcatc agcgtgaagg 351 ttttgct... This corresponds to the amino acid sequence <SEQ ID 1708; ORF 571.ng>: g571.pep (partial) 1 MRVFRVΝRFV VTVFGGGIGS AVPHAACVGK QAQADGACVF RTGHREEQLG 51 GDVGFFVAAV ADFFAVFVIH FRAERAAFVA AHRTQAAAVE VFKEGDFFGS 101 AVAARΝADFA AEHQREGFA... The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1709>: m571.seq 1 ATGGGTATTG CCGGCGCCGT AAATGTTTTG AACCCTGCCG CCGGTCGCGG 51 AACTGCTGTT GTCGTCGTAG GTTTTGCCGT CCCACACGCT GCCTGCGTCG 101 GCAAACAGGC TCAGGCGGAC GGTGCGCGCG TCTTTCGCGC CGGGCATCGG 151 GAAGAGCAGC TCGGCGGAGA CGTTGGCTTT TTTGTTGCCG CCGTAGCTGA 201 TTTTTTCGCC GTATTCGTCA TAGACTTTCG GACCGAGCGT GCCGCTTTCG 251 TATCCGCGCA CCGAACCCAG GCCGCCGCCG TAGAAGTTTT CAAAGAAGGG 301 GATTTCTTTG GTTCTGCCGT AGCCGCCCGC AATGCCGACT TCGCCGCCGA 351 GCATCAGCGT GAAGGTTTTG CTCAGGGGGA AGAACCAGGT TTGGTTGTGG 401 GTGGCGGAGT AGTATTGCAG TTTGCTGCCA GGCAGGGCGA TTTCGGCGTT 451 CACGCCCGTC AGGTAGCCGC GCGTCGGCCA TAA This corresponds to the amino acid sequence <SEQ ID 1710; ORF 571>: m571.pep 1 MGIAGAVΝVL ΝPAAGRGTAV VWGFAVPHA ACVGKQAQAD GARVFRAGHR 51 EEQLGGDVGF FVAAVADFFA VFVIDFRTER AAFVSAHRTQ AAAVEVFKEG 101 DFFGSAVAAR ΝADFAAEHQR EGFAQGEEPG LWGGGWLQ FAARQGDFGV 151 HARQVAARRP * m571/g571 93.1% identity in 102 aa overlap 10 20 30 40 50 60 m571.pep MGIAGAVNVLNPAAGRGTAWWGFAVPHAACVGKQAQADGARVFRAGHREEQLGGDVGF IIMIMIIIIIMIII g571 MRVFRVNRFWTVFGGGIGSAVPHAACVGKQAQADGACVFRTGHREEQLGGDVGF 10 20 30 40 50 70 80 90 100 110 120 m571.pep FVAAVADFFAVFVIDFRTERAAFVSAHRTQAAAVEVFKEGDFFGSAVAARNADFAAEHQR g571 FVAAVADFFAVFVIHFRAERAAFVAAHRTQAAAVEVFKEGDFFGSAVAARNADFAAEHQR 60 70 80 90 100 110 1 13300 114400 115500 116600 EGFAQGEEPGLWGGGWLQFAARQGDFGVHARQVAARRPX g571 EGFA 119 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1711>: a571. seq 1 ATGGGTATTG CCGGCGCCGT AAATGTTTTG AACCCTGCCG CCGGTCGCGG 51 AACTGCTGTT GTCGTCGTAG GTTTTGCCGT CCCACACGCT GCCTGCGTCG 101 GCAAACAGGC TCAGGCGGAC GGTGCGCGCG TCTTTCGCGC CGGGCATCGG 151 GAAGAGCAGC TCGGCGGAGA CGTTGGCTTT TTTGTTGCCG CCGTAGCTGA 201 TTTTTTCGCC GTATTCGTCA TACACTTTCG GACCGAGCGT GCCGCTTTCG 251 TATCCGCGCA CCGAACCCAG GCCGCCGCCG TAGAAGTTTT CAAAGAAGGG 301 GATTTCTTTG GTTCTGCCGT AGCCGCCCGC AATGCCGACT TCGCCGCCGA 351 GCATCAGCGT GAAGGTTTTG CTTAAGGGGA AGAACCAGGT TTGGTTGTGG 401 GTGGCGGAGT AGTATTGCAG TTTGCTGCCG GGCAGGGCGA TTTCGGCGTT 451 CACGCCCGTC AGGTAGCCGC GCGTCGGCCA TAA This corresponds to the amino acid sequence <SEQ ID 1712; ORF 571. a>: a571.pep 1 MGIAGAVΝVL ΝPAAGRGTAV VWGFAVPHA ACVGKQAQAD GARVFRAGHR 51 EEQLGGDVGF FVAAVADFFA VFVIHFRTER AAFVSAHRTQ AAAVEVFKEG 101 DFFGSAVAAR ΝADFAAEHQR EGFA*GEEPG LWGGGWLQ FAAGQGDFGV 151 HARQVAARRP * m571/a571 98.1% identity in 160 aa overlap 10 20 30 40 50 60 m571. pep MGIAGAVΝVLΝPAAGRGTAWWGFAVPHAACVGKQAQADGARVFRAGHREEQLGGDVGF II I II II II II II II II II II II I I II II II II II I II II II II II II II II II II I I M a571 MGIAGAVΝVLΝPAAGRGTAWWGFAVPHAACVGKQAQADGARVFRAGHREEQLGGDVGF 10 20 30 40 50 60 70 80 90 100 110 120 m571. pep FVAAVADFFAVFVIDFRTERAAFVSAHRTQAAAVEVFKEGDFFGSAVAARΝADFAAEHQR llllllllllllll II II II II II II II II II I I II II II II II II II I I II I I I II I I a571 FVAAVADFFAVFVIHFRTERAAFVSAHRTQAAAVEVFKEGDFFGSAVAARΝADFAAEHQR 70 80 90 100 110 120 130 140 150 160 m571. pep EGFAQGEEPGLWGGGVVLQFAARQGDFGVHARQVAARRPX II I I MMMMMMMMM a571 EGFAXGEEPGLWGGGWLQFAAGQGDFGVHARQVAARRPX 130 140 150 160 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 1713>: g572. seq . . 1 atgtgcgcca tcgtcggggc ggcggggctg ccttccgcgc tcgcagcggc 51 gcaaaaaggc aaaaccattt atctggcaaa oaaagaaacg ctggtggttt 101 ccggcgcgtt gtttatggaa accgcccgcg caaacggcgc ggcagtgttg 151 cccgtcgaca gcgaacacaa cgccattttc caagttttgc cgcgcgatta 201 cacagaccgt ctgaacgaac acggcatcga ttcgattatc ctgaccgctt 251 ccggcggccc gtttttaaca accgatttaa gcacgttcga cagcattacg 301 cccgagcagg cggtcaaaca ccccaattgg cgtatggggc gcaaaatctc 351 cgtcgattca gccactatgg caaacaaggg cttggaactg attgaagcgc 401 attggctgtt caactgtccg cccgacaaac tcgaagtcgt catccatccc 451 caatccgtga tacacagtat ggtgcgctac cgcgacggct ccgtgctggc 501 gcaactgggc aatcccgata tgcgaacgcc catcgcctat tgtttgggct 551 tgcccgagcg catcgattcg ggtgtcggca aactcgattt cggcgcattg 601 tccgcgctga ccttccaaaa gcccgacttc ggccgcttcc cctgcctgaa 651 gttcgcctat gaaaccataa acgcaggcgg agccgcgccc tgcgtattga 701 acgccgccaa cgaaaccgcc gtcgccgcct ttttggacgg acagattaag 751 tttaccgaca ttgccaaaac cgtcgcccac tgtcttgcac aagacttttc 801 aaacggcatg ggcgatatag aaggactgtt ggcgcaagat gcccggacac 851 gcgcacaagc gcgggcattt atcggcacao tgcgctga This corresponds to the amino acid sequence <SEQ ID 1714; ORF 572.ng>: g572.pep.. 1 MCAIVGAAGL PSALAAAQKG KTIYLANKET L SGALFME TARANGAAVL 51 PVDSEHNAIF QVLPRDYTDR LNEHGIDSII LTASGGPFLT TDLSTFDSIT 101 PEQΛVKHPNW RMGRKISVDS ATMANKGLEL IEAHWLFNCP PDKLEWIHP 151 QSVIHSMVRY RDGSVLAQLG NPDMRTPIAY CLGLPERIDS GVGKLDFGAL 201 SALTFQKPDF GRFPCLKFAY ETINAGGAAP CVL AANETA VAAFLDGQIK The following partial DNA sequence was identified in N meningitidis <SEQ ID 1715>: m572. seq.. 1 ATGTGCGCCA TCGTCGGGGC GGTGGGGCTG CCTTCCGCGC TCGCAGCGGC 51 GCAAAAAGGC AAAACCATTT ATCTGGCAAA CAAAGAAACG CTGGTGGTTT 101 CCGGCGCGTT GTTTATGGAA ACCGCCCGTG CAAACGGCGC GGCAGTGCTG 151 CCCGTCGACA GCGAACACAA CGCCGTTTTC CAAGTTTTGC CGCGCGATTA 201 CGCCGGCCGT CTGAACGAAC ACGGCATCGC TTCGATTATC CTGACCGCTT 251 CCGGCGGCCC GTTTCTGACC GCCGATTTAA ACACGTTCGA CCGCATTACG 301 CCCGCCCAAG CGGTCAAACA CCCCAATTGG CGTATGGGAC GCAAAATCTC 351 CGTCGATTCC GCCACCATGA TGAACAAAGG TTTGGAGCTG ATTGAAGCGC 401 ATTGGCTGTT CAACTGTCCG CCCGACAAAC TCGAAGTCGT CATCCATCCG 451 CAATCCGTGA TACACAGCAT GGTGCGCTAC CGCGACGGCT CCGTGCTGGC 501 GCAACTGGGC AATCCCGATA TGCGAACGCC CATCGCTTAT TGTTTGGGTT 551 TGCCCGAGCG CATCGATTCG GGTGTCGGCG ACCTGGATTT CGACGCATTG 601 TCCGCGCTGA CCTTCCAAAA GCCCGACTTT GACCGCTTCC CCTGCCTGAG 651 GCTCGCCTAT GAAGCCATGA ACGCAGGCGG AGCCGCGCCC TGCGTATTGA 701 ACGCCGCCAA CGAAGCCGCC GTCGCCGCCT TTTTGGACGG ACAGATTAAG 751 TTTACCGACA TTGCCAAAAC CGTCGCCCAC TGTCTTGCAC AAGACTTTTC 801 AGACGGCATA GGCGATATAG GGGGGCTCTT GGCGCAAGAT GCCCGGACAC 851 GCGCACAAGC GCGAGCATTT ATCGGCACAC TGCGCTGA This corresponds to the amino acid sequence <SEQ ID 1716; ORF 572>: m572.pep.. 1 MCAIVGAVGL PSALAAAQKG KTIYLANKET LWSGALFME TARANGAAVL 51 PVDSEHNAVF QVLPRDYAGR LNEHGIASII LTASGGPFLT ADLNTFDRIT 101 PAQAVKHPNW RMGRKISVDS ATMMNKGLEL IEAHWLFNCP PDKLEWIHP 151 QSVIHSMVRY RDGSVLAQLG NPDMRTPIAY CLGLPERIDS GVGDLDFDAL 201 SALTFQKPDF DRFPCLRLAY EAMNAGGAAP CVLNAANEAA VAAFLDGQIK 251 FTDIAKTVAH CLAQDFSDGI GDIGGLLAQD ARTRAQARAF IGTLR* m572/g572 92.9% identity in 295 aa overlap 10 20 30 40 50 60 m572.pep MCAIVGAVGLPSALAAAQKGKTIYLANKETLWSGALFMETARANGAAVLPVDSEHNAVF g572 MCAIVGAAGLPSALAAAQKGKTIYLANKETLWSGALFMETARANGAAVLPVDSEHNAIF 10 20 30 40 50 60 70 80 90 100 110 120 572 . pep QVLPRDYAGRLNEHGIASIILTAΞGGPFLTADLNTFDRITPAQAVKHPNWRMGRKISVDS M i l l : I I I 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 g572 QVLPRDYTDRLNEHGIDSIILTASGGPFLTTDLΞTFDΞITPEQAVKHPNWRMGRKISVDS 70 30 90 100 110 120 130 140 150 160 170 180 m572. pep ATMMNKGLELIEAHWLFNCPPDKLEWIHPQSVIHSMVRYRDGSVLAQLGNPDMRTPIAY M l g572 ATMANKGLELIEAHWLFNCPPDKLEWIHPQSVIHSMVRYRDGSVLAQLGNPDMRTPIAY 130 140 150 160 170 180 190 200 210 220 230 240 m572 . pep CLGLPERIDSGVGDLDFDALΞALTFQKPDFDRFPCLRLAYEAMNAGGAAPCVLNAANEAA I I I 1 1 1 1 1 1 1 1 1 1 1 1 g572 CLGLPERIDSGVGKLDFGALΞALTFQKPDFGRFPCLKFAYETINAGGAAPCVLNAANETA 190 200 210 220 230 240 250 260 270 280 290 m572.pep VAAFLDGQIKFTDIAKTVAHCLAQDFSDGIGDIGGLLAQDARTRAQARAFIGTLRX 1 1 1 1 1 1 I I 1 1 1 1 1 1 1 1 1 1 1 1 1 1 g572 VAAFLDGQIKFTDIAKTVAHCLAQDFSNGMGDIEGLLAQDARTRAQARAFIGTLRX 250 260 270 280 290 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1717>: a572. seq 1 ATGTGCGCCA TCGTCGGGGC GGTGGGGCTG CCTTCCGCGC TCGCAGCGGC 51 GCAAAAAGGC AAAACCATTT ATCTGGCGAA CAAAGAGACG CTGGTGGTTT 101 CCGGCGCGTT GTTTATGGAA ACCGCCCGTG CAAACGGCGC GGCAGTGCTG 151 CCCGTCGACA GCGAACACAA CGCCGTTTTC CAAGTTTTGC CGCGCGATTA 201 CACAGGTCGC CTGAACGAAC ACGGCATCGC TTCGATTATC CTGACCGCTT 251 CCGGCGGCCC GTTTCTGACC GCCGATTTAA ACACGTTCGA CAGCATTACG 301 CCCGACCAAG CGGTCAAACA CCCCAATTGG CGTATGGGAC GCAAAATCTC 351 CGTCGATTCC GCCACCATGA TGAACAAAGG TTTGGAGCTG ATTGAAGCGC 401 ATTGGCTGTT CAACTGTCCG CCCGACAAAC TCGAAGTCGT CATCCATCCG 451 CAATCTGTGA TACACAGCAT GGTGCGCTAC CGCGACGGCT CCGTGTTGGC 501 GCAACTGGGC AATCCCGATA TGCGAACGCC TATCGCTTAT TGTTTGGGTT 551 TGCCCGAGCG CATCGATTCG GGTGTCGGCG ACCTGGATTT CGACGCATTG 601 TCCGCGCTGA CCTTCCAAAA GCCCGACTTT GACCGCTTCC CCTGCCTGAA 651 GCTCGCCTAT GAAGCCATGA ACGCAGGCGG AGCCGCGCCC TGCGTATTGA 701 ACGCCGCCAA CGAAGCCGCC GTCGCCGCCT TTTTGGACGG ACAGATTAAG 751 TTTACCGACA TTGCCAAAAC CGTCGCCCAT TGTCTTTCAC AAGACTTTTC 801 AGACGGCATA GGCGACATAG GGGGGCTCTT GGCGCAAGAT GCCCGGACAC 851 GCGCACAAGC GCGGGCATTT ATCGGCACAC TGCGCTGA This corresponds to the amino acid sequence <SEQ ID 1718; ORF 572. a>: a572.pep 1 MCAIVGAVGL PSALAAAQKG KTIYLANKET LWSGALFME TARANGAAVL 51 PVDSEHNAVF QVLPRDYTGR LNEHGIASII LTASGGPFLT ADLNTFDSIT 101 PDQAVKHPNW RMGRKISVDS ATMMNKGLEL IEAHWLFNCP PDKLEWIHP 151 QSVIHSMVRY RDGSVLAQLG NPDMRTPIAY CLGLPERIDS GVGDLDFDAL 201 SALTFQKPDF DRFPCLKLAY EAMNAGGAAP CVLNAANEAA VAAFLDGQIK 251 FTDIAKTVAH CLSQDFSDGI GDIGGLLAQD ARTRAQARAF IGTLR* m572/a572 98.3% identity in 295 aa overlap 10 20 30 40 50 60 m572 . pep MCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARANGAAVLPVDSEHNAVF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a572 MCAIVGAVGLPΞALAAAQKGKTIYLANKETLWSGALFMETARANGAAVLPVDSEHNAVF 10 20 30 40 50 60 70 80 90 100 110 120 m572.pep QVLPRDYAGRLNEHGIASIILTASGGPFLTADLNTFDRITPAQAVKHPNWRMGRKISVDS I II I II M II II I II I II I II II II II II I II I II I I III II II I I I I I I II I I I I II a572 QVLPRDYTGRLNEHGIASIILTASGGPFLTADLNTFDSITPDQAVKHPNWRMGRKISVDS 70 80 90 100 110 120 130 140 150 160 170 180 m572.pep ATMMNKGLELIEAHWLFNCPPDKLEWIHPQSVIHSMVRYRDGSVLAQLGNPDMRTPIAY II II I I II I II II I II I I II II I II I II II II I II II II II I II II I II II I II I I I I II a572 ATMMNKGLELIEAHWLFNCPPDKLEWIHPQSVIHSMVRYRDGSVLAQLGNPDMRTPIAY 130 140 150 160 170 180 190 200 210 220 230 240 m572. pep CLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLRLAYEAMNAGGAAPCVLNAANEAA MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a572 CLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKLAYEAMNAGGAAPCVLNAANEAA 190 200 210 220 230 240 250 260 270 280 290 m572. pep VAAFLDGQIKFTDIAKTVAHCLAQDFSDGIGDIGGLLAQDARTRAQARAFIGTLRX II II I II II II II II II II II I : II II II II II II II II II II II I II II I II II I a572 VAAFLDGQIKFTDIAKTVAHCLSQDFSDGIGDIGGLLAQDARTRAQARAFIGTLRX 250 260 270 280 290 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1719>: g573.seq.. 1 atgccctgtt tgtgccgcct taatcgcaat atcggcagtt tccaaatcac 51 gaatctcacc gaccataatg atgtccgggt cctgacgcag gaaagacttc 101 aaagcagcgg caaaagtcag accctgctta tcattgacgt taacctgatt 151 gatgcccggc aggttaatct cggcagggtc ttccgccgtt gcaatattta 201 ccgactccgt attcaaaata ttcaaacagg tatagagcga caccgtctta 251 cccgaacccg tcggaccggt taccagcacc atcccgtaag gacggtgaat 301 cgcttccaac aacaattttt tctggaacgg ctcaaaaccg agctggtcga 351 tgttcaaaga ogcggcatcg gaattcaaaa tccgcatcac gaccttttcg 401 ccaaacagcg tcggcaatgt gctgacacgg aaatcgacag gcttgccgcc 451 cttttgaaag gtcagctgca tcctaccgtc ctgcggtatc cgtttttcgg 501 aaatgtccaa acgcgacatt accttaatcc gggaagcaag ctgccccctt 551 accgcaatgg gcggctgaac cacctcgcgg agctgcccgt ccacacggaa 601 acggatacgc gcattgtgtt cgtaaaactc gaaatggatg tcggatgccc 651 cgctacgcaa ggcatccgac aaagttttat ggataaacct cggaacaggg 701 ccgtcttctg cctcctcgtc gtcgatatac agggtgtggc tttcctcttc 751 ctcttgcccc tccccaagct cctgaagcag cgatgtcgaa cgcgaaccca 801 cccaatcgag caaacccgcc aactggtcat cctcgacaat gaccaactca 851 accgcaatcc ctgcggcaga aaccgttttc tgaatttgcg gcatctgggt 901 cggatcggaa accgcaaaaa atactttgtc gcccccacgg aaaaccggca 951 cacagtggaa ctccaccatc tgctcctccg tcaacacccc catcagcacc 1001 ctgtggcgcg gataatgacg caaatcaaga atcgaataac tgaacaccct 1051 cgcaatcaat gccgcaagcg acttgggcga aatgacaccg tctga This corresponds to the amino acid sequence <SEQ ID 1720; ORF 573.ng>: g573.pep.. 1 MPCLCRLΝRΝ IGSFQITΝLT DHΝDVRVLTQ ERLQSSGKSQ TLLIIDVΝLI 51 DARQVΝLGRV FRRCΝIYRLR IQΝIQTGIER HRLTRTRRTG YQHHPVRTVΝ 101 RFQQQFFLER LKTELVDVQR RGIGIQΝPHH DLFAKQRRQC ADTEIDRLAA 151 LLKGQLHPTV LRYPFFGΝVQ TRHYLΝPGSK LPPYRΝGRLΝ HLAELPVHTE 201 TDTRIVFVKL EMDVGCPATQ GIRQSFMDKP RΝRAVFCLLV VDIQGVAFLF 251 LLPLPKLLKQ RCRTRTHPIE QTRQLVILDΝ DQLΝRΝPCGR ΝRFLΝLRHLG 301 RIGΝRKKYFV APTE RHTVE LHHLLLRQHP HQHPVARIMT QIKΝRITEHP 351 RΝQCRKRLGR ΝDTV* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1721>: m573.seq.. 1 ATGCCCTGTT TGTGCCGCCT TAATCGCAAT ATCGGCAGTT TCCAAATCAC 51 GAATCTCACC GACCATAATG ATGTCCGGGT CCTGACGCAG GAAAGACTTC 101 AAAGCAGCGG CAAAAGTCAG GCCCTGCTTA TCATTGACGT TAACCTGATT 151 GATGCCCGGC AGGTTAATCT CGGCAGGGTC TTCCGCCGTT GCAATATTTA 201 CCGACTCCGT ATTCAAAATA TTCAAACAGG TATAGAGCGA CACCGTCTTA 251 CCCGAACCCG TCGGACCGGT TACCAGCACC ATCCCGTAGG GACGGTGAAT 301 CGCTACCAAC aCa TTTTT TCTGAAACGG CTCAAAACCG AGCTGGTCGA 351 TGTTCAAAGA CGCGGCATCG GAATTCAAAA TCCGCATCAC GACCTTTTCG 401 CCAAACAGCG TCGGCAATGT GCTGACACGG AAATCGACAG GCTTGCCGCC 451 CTTTTGAAAG GTCAGCTGCA TCCTGCCGTC CTGCGGTATC CGTTTTTCGG 501 AAATGTCCAA ACGCGACATT ACCTTAATCC GTGAAGCAAG CTGCCCCCTT 551 ACCGCAATGG GCGGCTGAAC CACCTCGCGG AGCTGCCCGT CCACACGGAA 601 ACGGATACGG GCATTGTGTT CGTAAAACTC GAAATGGATG TCCGATGCCC 651 CGCTGCGCAA GGCATCCGAC AAAGTCTTAT GGATAAACCT CGGAACAGGG 701 CCGTCTTCTG CCTCCTCGTT GTCGATATAC AGGGTGTGGC TTTCCTCTTC 751 CTCCTGCCCC TCCCCAAGCT CCTGAAGCAG CGATGTCGAA CGCGAACCCA 801 CCCAATCGAG CAAACCCGCC AACTGGTCAT CCTCGACAAT GACCAACTCA 851 ACCTCAATCC CTGCGGCAGA AACGGTTTTC TGAATTTGCG GCATCTGTGT 901 CGGATCGGAA ACCGCAAAAA ATACTTTGTC GCCCCGACGG AAAACCGGCA 951 CACAGTGGAA CTCCACCATC TGCTCCTCCG TCAACACCCC CATCAGCACC 1001 CTGTGGCGCG GATAATGACG CAAATCAAGA ATCGAATAAC TGAACACCCT 1051 CGCAATCAAT GCCGCAAGCG ACTTGGGCGA AATGACACCG TCTGA This corresponds to the amino acid sequence <SEQ ID 1722; ORF 573>: m573.pep.. 1 MPCLCRLNRN IGSFQITNLT DHNDVRVLTQ ERLQSSGKSQ ALLIIDVNLI 51 DARQVNLGRV FRRCNIYRLR IQNIQTGIER HRLTRTRRTG YQHHPVGTVN 101 RYQHXFFLKR LKTELVDVQR RGIGIQNPHH DLFAKQRRQC ADTEIDRLAA 151 LLKGQLHPAV LRYPFFGNVQ TRHYLNP*SK LPPYRNGRLN HLAELPVHTE 201 TDTGIVFVKL EMDVRCPAAQ GIRQSLMDKP RNRAVFCLLV VDIQGVAFLF 251 LLPLPKLLKQ RCRTRTHPIE QTRQLVILDN DQLNLNPCGR NGFLNLRHLC 301 RIGNRKKYFV APTENRHTVE LHHLLLRQHP HQHPVARIMT QIKNRITEHP 351 RNQCRKRLGR NDTV* m573/g573 95.9% identity in 364 aa overlap 10 20 30 40 50 60 m573.pep MPCLCRLNRNIGSFQITNLTDHNDVRVLTQERLQSSGKSQALLIIDVNLIDARQVNLGRV g573 MPCLCRLNRNIGSFQITNLTDHNDVRVLTQERLQSSGKSQTLLIIDVNLIDARQVNLGRV 10 20 30 40 50 60 70 80 90 100 110 120 m573. pep FRRCNIYRLRIQNIQTGIERHRLTRTRRTGYQHHPVGTVNRYQHXFFLKRLKTELVDVQR I I I M h I I I M I I M I I M M g573 FRRCNIYRLRIQNIQTGIERHRLTRTRRTGYQHHPVRTVNRFQQQFFLERLKTELVDVQR 70 80 90 100 110 120 130 140 150 160 170 180 m573.pep RGIGIQNPHHDLFAKQRRQCADTEIDRLAALLKGQLHPAVLRYPFFGNVQTRHYLNPXSK I I g573 RGIGIQNPHHDLFAKQRRQCADTEIDRLAALLKGQLHPTVLRYPFFGNVQTRHYLNPGSK 130 140 150 160 170 180 190 200 210 220 230 240 m573.pep LPPYRNGRLNHLAELPVHTETDTGIVFVKLEMDVRCPAAQGIRQSLMDKPRNRAVFCLLV g573 LPPYRNGRLNHLAELPVHTETDTRIVFVKLEMDVGCPATQGIRQSFMDKPRNRAVFCLLV 190 200 210 220 230 240 250 260 270 280 290 300 m573.pep VDIQGVAFLFLLPLPKLLKQRCRTRTHPIEQTRQLVILDNDQLNLNPCGRNGFLNLRHLC g573 VDIQGVAFLFLLPLPKLLKQRCRTRTHPIEQTRQLVILDNDQLNRNPCGRNRFLNLRHLG 250 260 270 280 290 300 310 320 330 340 350 360 m573. pep RIGNRKKYFVAPTENRHTVELHHLLLRQHPHQHPVARIMTQIKNRITEHPRNQCRKRLGR I 'M Ml MM! MM! Ill MMM M I M i,-! I g573 RIGNRKKYFVAPTENRHTVELHHLLLRQHPHQHPVARIMTQIKNRITEHPRNQCRKRLGR 310 320 330 340 350 360 m573.pep NDTVX Mill g573 NDTVX The following partial DNA sequence was identified in N. meningitidis <SEQ ID 1723>: a.573. seq 1 ATGCCCTGTT TGTGCCGCCT TAATCGCAAT ATCGGCAGTT TCCAAATCAC 51 GAATCTCACC GACCATAATG ATGTCCGGGT CCTGACGCAG GAAAGACTTC 101 AAAGCAGCGG CAAAAGTCAG ACCCTGCTTA TCATTGACGT TAACCTGATT 151 GATGCCCGGC AGGTTAATCT CGGCAGGGTC TTCCGCCGTT GCAATATTTA 201 CCGACTCCGT ATTCAAAATA TTCAAACAGG TATAGAGCGA CACCGTCTTA 251 CCCGAACCCG TCGGACCGGT TACCAGCACC ATCCCGTAGG GACGGTGAAT 301 CGCTTCCAAC AACAATTTTT TCTGAAACGG CTCAAAACCG AGCTGGTCGA 351 TGTTCAAAGA CGCGGCATCG GAATTCAAAA TCCGCATCAC GACCTTTTCG 401 CCAAACAGCG TCGGCAATGT GCTGACACGG AAATCGACAG GCTTGCCGCC 451 CTTTTGAAAG GTCAGCTGCA TCCTGCCGTC CTGCGGTATC CGTTTTTCGG 501 AAATGTCCAA ACGCGACATT ACCTTAATCC GGGAAGCAAG CTGCCCCCTT 551 ACCGCAATGG GCGGCTGAAC CACCTCGCGG AGCTGCCCGT CCACACGGAA 601 ACGGATACGG GCATTGTGTT CGTAAAACTC GAAATGGATG TCCGATGCCC 651 CGCTGCGCAA GGCATCCGAC AAAGTCTTAT GGATAAACCT CGGAACAGGG 701 CCGTCTTCTG CCTCCTCGTT GTCGATATAC AGGGTGTGGC TTTCCTCTTC 751 CTCCTGCCCC TCCCCAAGCT CCTGAAGCAG CGATGTCGAA CGCGAACCCA 801 CCCAATCGAG CAAACCCGCC AACTGGTCAT CCTCGACAAT GACCAACTCA 851 ACCTCAATCC CTGCGGCAGA AACGGTTTTC TGAATTTGCG GCATCTGTGT 901 CGGATCGGAA ACCGCAAAAA ATACTTTGTC GCCCCGACGG AAAACCGGCA 951 CACAGTGGAA CTCCACCATC TGCTCCTCCG TCAACACCCC CATCAGCACC 1001 CTGTGGCGCG GATAATGACG CAAATCAAGA ATCGAATAAC TGAACACCCT 1051 CGCAATCAAT GCCGCAAGCG ACTTGGGCGA AATGACACCG TCTGA This corresponds to the amino acid sequence <SEQ ID 1724; ORF 573. a>: a573.pep 1 MPCLCRLNRN IGSFQITNLT DHNDVRVLTQ ERLQSSGKSQ TLLIIDVNLI 51 DARQVNLGRV FRRCNIYRLR IQNIQTGIER HRLTRTRRTG YQHHPVGTVN 101 RFQQQFFLKR LKTELVDVQR RGIGIQNPHH DLFAKQRRQC ADTEIDRLAA 151 LLKGQLHPAV LRYPFFGNVQ TRHYLNPGSK LPPYRNGRLN HLAELPVHTE 201 TDTGIVFVKL EMDVRCPAAQ GIRQSLMDKP RNRAVFCLLV VDIQGVAFLF 251 LLPLPKLLKQ RCRTRTHPIE QTRQLVILDN DQLNLNPCGR NGFLNLRHLC 301 RIGNRKKYFV APTENRHTVE LHHLLLRQHP HQHPVARIMT QIKNRITEHP 351 RNQCRKRLGR NDTV* m573/a573 98.6% identity in 364 aa overlap 10 20 30 40 50 60 m573. pep MPCLCRLNRNIGSFQITNLTDHNDVRVLTQERLQSSGKSQALLIIDVNLIDARQVNLGRV MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a573 MPCLCRLNRNIGSFQITNLTDHNDVRVLTQERLQSSGKSQTLLIIDVNLIDARQVNLGRV 10 20 30 40 50 60 70 80 90 100 110 120 m573. pep FRRCNIYRLRIQNIQTGIERHRLTRTRRTGYQHHPVGTVNRYQHXFFLKRLKTELVDVQR I II II II II II I II II II I II II I I I II I II II II II II II : I : lllllllllllllll a573 FRRCNIYRLRIQNIQTGIERHRLTRTRRTGYQHHPVGTVNRFQQQFFLKRLKTELVDVQR 70 80 90 100 110 120 130 140 150 160 170 180 m573. pep RGIGIQNPHHDLFAKQRRQCADTEIDRLAALLKGQLHPAVLRYPFFGNVQTRHYLNPXSK I II II II II II I II II I II II I II II II II II I II II II II II II II II I I I I II II II a573 RGIGIQNPHHDLFAKQRRQCADTEIDRLAALLKGQLHPAVLRYPFFGNVQTRHYLNPGSK 130 140 150 160 170 180 190 200 210 220 230 240 m573.pep LPPYRNGRLNHLAELPVHTETDTGIVFVKLEMDVRCPAAQGIRQSLMDKPRNRAVFCLLV I II II II II II I I II II II I I I II I II I I I II I II II II II I I II I II II II I II II M I a573 LPPYRNGRLNHLAELPVHTETDTGIVFVKLEMDVRCPAAQGIRQSLMDKPRNRAVFCLLV 190 200 210 220 230 240 250 260 270 280 290 300 m573. pep VDIQGVAFLFLLPLPKLLKQRCRTRTHPIEQTRQLVILDNDQLNLNPCGRNGFLNLRHLC II II I II I I II II II II II II II II I I I I I I II II II I II I I I II II II I I I I I I I M I I a573 VDIQGVAFLFLLPLPKLLKQRCRTRTHPIEQTRQLVILDNDQLNLNPCGRNGFLNLRHLC 250 260 270 280 290 300 310 320 330 340 350 360 m573. pep RIGNRKKYFVAPTENRHTVELHHLLLRQHPHQHPVARIMTQIKNRITEHPRNQCRKRLGR MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a573 RIGNRKKYFVAPTENRHTVELHHLLLRQHPHQHPVARIMTQIKNRITEHPRNQCRKRLGR 310 320 330 340 350 360 m573.pep NDTVX I I II I a573 NDTVX The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1725>: g574.seq 1 atgctgccga atctgccaaa cagccttaag aaagccgata tggacaacga 51 attgtggatt atcctgctgc cgattatcct tttgcccgtc ttcttcacga 101 tgggctggtt tgccgcccgc gtggatatga aaaccgtatt gaagcaggca 151 aaaagcatcc cttcgggatt ttataaaagc ctggacgctt tggtcgaccg 201 caacagcggg cgcgcggcaa gggagttggc ggaagtcgtc gacggccggc 251 cgcaatcgta tgatttgaac cttaccctcg gcaaacttta ccgtcagcgc 301 ggcgaaaacg acaaagccat caacatacac cggacaatgc tcgattctcc 351 cgatacggtc ggcgaaaagc gcgcgcgcgt cctgtttgaa ttggogcaaa 401 actaccaaag cgcgggtttg gtcgatcgtg ccgaacagat ttttttgggg 451 ctgcaagacg gtgaaatggc gcgtgaagcc agacagcacc tgctcaatat 501 ctaccagcag gacagggatt gggaaaaagc ggttgaaacc gcccaacttc 551 ttagtcacga cgaacagaca tatcagtttg agattgcaca gttttattgc 601 gaacttgccc aagccgcgct gttcaagtcc aatttcgatg ccgcgcgttt 651 caatgtcggc aaggcactcg aagccaacaa aaaatgcacc cgcgccaaca 701 tgattttggg cgacattgaa caccgacaag gcaatttccc tgccgccgtc 751 gaagcctatg ccgccatcga gcagcaaaac catgcatact tgagcatggt 801 cggcgagaag ctttacgaag cctatgccgc gcagggaaaa cctgaagaag 851 gcttgaaccg tctgacagga tatatgcaga cgtttcccga acttgacctg 901 atcaatgtcg tgtacgagaa atccctgctg cttaagggcg agaaagaagc 951 cgcgcaaacc gccgtcgagc ttgtccgccg caagcccgac cttaacggcg 1001 tgtaccgcct gctcggtttg aaactcagcg atttggatcc ggcttggaaa 1051 gccgatgccg acatgatgcg ttcggttatc ggacggcagc tccagcgcag 1101 cgtgatgtac cgttgccgca actgccactt caaatcccaa gtctttttct 1151 ggcactgtcc cgcctgcaac aaatggcaga cgtttacgcc gaataaaatc 1201 gaagtttaa This corresponds to the amino acid sequence <SEQ ID 1726; ORF 574.ng>: g574.pep.. 1 MLPΝLPΝSLK KADMDΝELWI ILLPIILLPV FFTMGWFAAR VDMKTVLKQA 51 KSIPSGFYKS LDALVDRΝSG RAARELAEW DGRPQSYDLΝ LTLGKLYRQR 101 GEΝDKAIΝIH RTMLDSPDTV GEKRARVLFE LAQΝYQSAGL VDRAEQIFLG 151 LQDGEMAREA RQHLLΝIYQQ DRDWEKAVET AQLLSHDEQT YQFEIAQFYC 201 ELAQAALFKS ΝFDAARFΝVG KALEAΝKKCT RAΝMILGDIE HRQGΝFPAAV 251 EAYAAIEQQΝ HAYLSMVGEK LYEAYAAQGK PEEGLΝRLTG YMQTFPELDL 301 IΝWYEKSLL LKGEKEAAQT AVELVRRKPD LΝGVYRLLGL KLSDLDPAWK 351 ADADMMRSVI GRQLQRSVMY RCRΝCHFKSQ VFFWHCPACΝ KWQTFTPΝKI 401 EV* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1727>: m574.seq.. 1 ATGCGCCCGA ATCTACCAAA CAGCCTTAAG AAAGCCGATA TGGACAACGA 51 ATTGTGGATT ATCCTGCTGC CGATTATCCT TTTGCCCGTC TTCTTCGCGA 101 TGGGCTGGTT TGCCGCCCGC GTGGATATGA AAACCGTATT GAAGCAGGCA 151 AAAAGCATCC CTTCGGGATT TTATAAAAGC TTGGACGCTT TGGTCGACCG 201 CAACAGCGGG CGCGCGGCAA GGGAGTTGGC GGAAGTCGTC GACGGCCGGC 251 CGCAATCGTA TGATTTGAAC CTCACCCTCG GCAAACTTTA CCGCCAGCGT 301 GGCGAAAACG ACAAAGCCAT CAACATACAC CGGACAATGC TCGATTCTCC 351 CGATACGGTC GGCGAAAAGC GCGCGCGCGT CCTGTTTGAA TTGGCGCAAA 401 ACTACCAAAG TGCGGGGTTG GTCGATCGTG CCGAACAGAT TTTTTTGGGG 451 CTGCAAGACG GTAAAATGGC GCGTGAAGCC AGACAGCACC TGCTCAATAT 501 CTACCAACAG GACAGGGATT GGGAAAAAGC GGTTGAAACC GCCCGGCTGC 551 TCAGCCATGA CGATCAGACC TATCAGTTTG AAATCGCCCA GTTTTATTGC 601 GAACTTGCCC AAGCCGCGCT GTTCAAGTCC AATTTCGATG TCGCGCGTTT 651 CAATGTCGGC AAGGCACTCG AAGCCAACAA AAAATGCACC CGCGCCAACA 701 TGATTTTGGG CGACATCGAA CACCGACAAG GCAATTTCCC TGCCGCCGTC 751 GAAGCCTATG CCGCCATCGA GCAGCAAAAC CATGCATACT TGAGCATGGT 801 CGGCGAGAAG CTTTACGAAG CCTATGCCGC GCAGGGAAAA CCTGAAGAAG 851 GCTTGAACCG TCTGACAGGA TATATGCAGA CGTTTCCCGA ACTTGACCTG 901 ATCAATGTCG TGTACGAGAA ATCCCTGCTG CTTAAGTGCG AGAAAGAAGC 951 CGCGCAAACC GCCGTCGAGC TTGTCCGCCG CAAGCCCGAC CTTAACGGCG 1001 TGTACCGCCT GCTCGGTTTG AAACTCAGCG ATATGAATCC GGCTTGGAAA 1051 GCCGATGCCG ACATGATGCG TTCGGTTATC GGACGGCAGC TACAGCGCAG 1101 CGTGATGTAC CGTTGCCGCA ACTGCCACTT CAAATCCCAA GTCTTTTTCT 1151 GGCACTGCCC CGCCTGCAAC AAATGGCAGA CGTTTACCCC GAATAAAATC 1201 GAAGTTTAA This corresponds to the amino acid sequence <SEQ ID 1728; ORF 574>: m574.pep.. 1 MRPNLPNSLK KADMDNELWI ILLPIILLPV FFAMGWFAAR VDMKTVLKQA 51 KSIPSGFYKS LDALVDRNSG RAARELAEW DGRPQSYDLN LTLGKLYRQR 101 GENDKAINIH RTMLDSPDTV GEKRARVLFE LAQNYQΞAGL VDRAEQIFLG 151 LQDGKMAREA RQHLLNIYQQ DRDWEKAVET ARLLSHDDQT YQFEIAQFYC 201 ELAQAALFKS NFDVARFNVG KALEANKKCT RANMILGDIE HRQGNFPAAV 251 EAYAAIEQQN HAYLSMVGEK LYEAYAAQGK PEEGLNRLTG YMQTFPELDL 301 INWYEKSLL LKCEKEAAQT AVELVRRKPD LNGVYRLLGL KLSDMNPAWK 351 ADADMMRSVI GRQLQRSVMY RCRNCHFKSQ VFFWHCPACN KWQTFTPNKI 401 EV* m573/g573 97.8% identity in 402 aa overlap 10 20 30 40 50 60 m574.pep MRPNLPNSLKKADMDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGFYKS I g574 MLPNLPNSLKKADMDNELWIILLPIILLPVFFTMGWFAARVDMKTVLKQAKSIPΞGFYKS 10 20 30 40 50 60 70 80 90 100 110 120 m574 . pep LDALVDRNSGRAARELAEWDGRPQSYDLNLTLGKLYRQRGENDKAINIHRTMLDSPDTV g574 LDALVDRNSGRAARELAEWDGRPQΞYDLNLTLGKLYRQRGENDKAINIHRTMLDSPDTV 70 80 90 100 110 120 130 140 150 160 170 180 m574 . pep GEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGKMAREARQHLLNIYQQDRDWEKAVET g574 GEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGEMAREARQHLLNIYQQDRDWEKAVET 130 140 150 160 170 180 190 200 210 220 230 240 m574.pep ARLLSHDDQTYQFEIAQFYCELAQAALFKSNFDVARFNVGKALEANKKCTRANMILGDIE g574 AQLLSHDEQTYQFEIAQFYCELAQAALFKSNFDAARFNVGKALEANKKCTRANMILGDIE 190 200 210 220 230 240 250 260 270 280 290 300 m574.pep HRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLTGYMQTFPELDL g574 HRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLTGYMQTFPELDL 250 260 270 280 290 300 310 320 330 340 350 360 m574 . pep INWYEKSLLLKCEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDMNPAWKADADMMRSVI I M M ' M g574 INWYEKSLLLKGEKEAAQTAVELVRRKPDLNGVYRLLGLKLΞDLDPAWKADADMMRSVI 310 320 330 340 350 360 370 380 390 400 m574 . pep GRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEVX g574 GRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEVX 370 380 390 400 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1729>: a574. seq 1 ATGCGCCCGA ATCTGCCAAA CAGCCTTGAG AAAGCCGATA TGGACAATGA 51 ATTGTGGATT ATCCTGCTGC CGATTATCCT TTTGCCCGTT TTCTTCGCGA 101 TGGGCTGGTT TGCCGCCCGC GTGGATATGA AGACTGTATT AAAGCAGGCA 151 AAAAGCATAC CGTCGGGATT TTATAAAAGT CTGGATGCCT TGGTTGACCG 201 CAACAGCGGG CGCGCGGCAA GGGAGTTGGC GGAAGTCGTC GACGGCCGGC 251 CGCAATCGTA TGATTTGAAC CTCACCCTCG GCAAACTTTA CCGCCAGCGT 301 GGCGAAAACG ACAAAGCCAT CAATATGCAC CAAACATTGC TTGACTCTCC 351 CGATACAACC GGAGCCAAGC GCGCGCGCGT CCTGTTTGAA TTGGCGCAAA 401 ACTACCAAAG TGCGGGGTTG GTCGATCGTG CCGAACAGAT TTTTTTGGGG 451 CTGCAAGACG GTGAAATGGC GCGTGAAGCC AGACAGCACC TGCTCAATAT 501 CTACCAACAG GACAGGGATT GGGAAAAAGC GGTTGAAACC GCCCGGCTGC 551 TCAGCCATGA CGATCAGACC TATCAGTTTG AAATCGCCCA GTTTTATTGC 601 GAACTTGCCC AAGCCGCGCT GTTCAAGTCC AATTTCGATG CCGCGCGTTT 651 CAATGTCGGC AAGGCACTCG AAGCCAACAA AAAATGCACC CGCGCCAACA 701 TGATTTTGGG CGACATCGAA CACCGACAAG GCAATTTCCC TGCCGCCGTC 751 GAAGCCTATG CCGCCATCGA GCAGCAAAAC CATGCATACT TGAGTATGGT 801 CGGCGAGAAG CTTTACGAAG CCTATGCCGC GCAGGGAAAA CCTGAAGAAG 851 GCTTGAACCG TCTGACAGGA TATATGCAGA CGTTTCCCGA ACTTGACCTG 901 ATCAATGTCG TGTACGAGAA ATCCCTGCTG CTTAAGTGCG AGAAAGAAGC 951 CGCGCAAACC GCCGTCGAGC TTGTCCGCCG CAAGCCCGAC CTCAACGGCG 1001 TGTACCGCCT GCTTGGTTTG AAACTCAGCG ATTTGGATCC GGCTTGGAAA 1051 GCCGATGCCG ATATGATGCG TTCGGTTATC GGACGGCAGC TACAGCGCAG 1101 CGTGATGTAC CGGTGCCGAA ACTGCCACTT CAAATCACAA GTCTTTTTCT 1151 GGCATTGTCC TGCCTGCAAC AAATGGCAGA CGTTTACGCC AAACAAAATC 1201 GAAGTTTAA This corresponds to the amino acid sequence <SEQ ID 1730; ORF 574.a>: a574.pep 1 MRPΝLPΝSLE KADMDΝELWI ILLPIILLPV FFAMGWFAAR VDMKTVLKQA 51 KSIPSGFYKS LDALVDRΝSG RAARELAEW DGRPQSYDLΝ LTLGKLYRQR 101 GEΝDKAIΝMH QTLLDSPDTT GAKRARVLFE LAQΝYQSAGL VDRAEQIFLG 151 LQDGEMAREA RQHLLΝIYQQ DRDWEKAVET ARLLSHDDQT YQFEIAQFYC 201 ELAQAALFKS ΝFDAARFΝVG KALEAΝKKCT RAΝMILGDIE HRQGΝFPAAV 251 EAYAAIEQQΝ HAYLSMVGEK LYEAYAAQGK PEEGLΝRLTG YMQTFPELDL 301 IΝWYEKSLL LKCEKEAAQT AVELVRRKPD LΝGVYRLLGL KLSDLDPAWK 351 ADADMMRSVI GRQLQRSVMY RCRΝCHFKSQ VFFWHCPACΝ KWQTFTPΝKI 401 EV* m574/a574 97.5% identity in 402 aa overlap 10 20 30 40 50 60 m574. pep MRPΝLPΝSLKKADMDΝELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGFYKS MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a574 MRPΝLPΝSLEKADMDΝELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGFYKS 10 20 30 40 50 60 70 80 90 100 110 120 m574. pep LDALVDRΝSGRAARELAEWDGRPQSYDLΝLTLGKLYRQRGEΝDKAIΝIHRTMLDSPDTV II II II II II II II II I II II II II II I I II II I II II II I II II II I : I : I : I I I II I : a574 LDALVDRΝSGRAARELAEWDGRPQSYDLΝLTLGKLYRQRGEΝDKAIΝMHQTLLDSPDTT 70 80 90 100 110 120 130 140 150 160 170 180 m574. pep GEKRARVLFELAQΝYQSAGLVDRAEQIFLGLQDGKMAREARQHLLΝIYQQDRDWEKAVET I I II II II II I I I II I I II II II II II I II II I : II II II I II II II I II II II I II II a574 GAKRARVLFELAQΝYQSAGLVDRAEQIFLGLQDGEMAREARQHLLΝIYQQDRDWEKAVET 130 140 150 160 170 180 190 200 210 220 230 240 m574.pep ARLLSHDD Q TYQFEIAQFYCELAQAALFKSΝFDVARFΝVGKALEAΝKKCTRAΝMILGDIE II II II II I II II I I II I I I I I I I I II I I I II I : II I M I I I M I I I I I I M M I I M M a574 ARLLSHDD Q TYQFEIAQFYCELAQAALFKSΝFDAARFΝVGKALEAΝKKCTRAΝMILGDIE 190 200 210 220 230 240 250 260 270 280 290 300 m574. pep HRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLTGYMQTFPELDL I II II II II II II I II I I II II II II II II II I II II II II II II II II II I II I I I I I I a574 HRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLTGYMQTFPELDL 250 260 270 280 290 300 310 320 330 340 350 360 m574. pep INWYEKSLLLKCEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDMNPAWKADADMMRSVI II I I II II II II II II I II II II II II I II I II I II II II I II I : : II II II I II I II I I a574 INWYEKSLLLKCEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDLDPAWKADADMMRSVI 310 320 330 340 350 360 370 380 390 400 m574. pep GRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEVX II I II II I II I II II II II II II II II II I II I I II II II II I a574 GRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEVX 370 380 390 400 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1731>: g575.seq (partial) 1 ..atgcogtgcc tccgccggca agcagcaagg tgtacgaacc gccgaacaga 51 ccgtcaaaca gtccgctttc ggtttcttct tcggcagaaa cctgttcgac 101 aggttcggca acgggttcgg cggcaacttc actggctgtt tccgcaacag 151 gttcggaaac ggtgttaccg gtttcgtcgg tcggcgtgtc gatggcagaa 201 gcggcggctt cttggggggg cggattcggc agcggtttcc gatgcggcag 251 tatttgcagc gggtacaggt ccgggttggc gttctgtcgc cgaagccgga 301 gtttcggaca ctgcgggttt gggttcgggt cgaacggccg gtttttccgc 351 ttttgcttcg ggcgcggcaa cttttgcttc aggtttttca accggttttt 401 cgacaggttt ctctatcggt ttctccacag ttgcctgttt ggacggttca 451 gacggcatgg atgcagtttc ggctttgggt ttcgccgttt gcggtttggg 501 ttgttccgct ttgatttttt tgggtgctgc cgctttgatc ctgttcagat 551 tcggaatgtg a* This corresponds to the amino acid sequence <SEQ ID 1732; ORF 575.ng>: g575.pep (partial) 1 ..MPCLRRQAAR CTΝRRTDRQT VRFRFLLRQK PVRQVRQRVR RQLHWLFPQQ 51 VRKRCYRFRR SACRWQKRRL LGGADSAAVS DAAVFAAGTG PGWRSVAEAG 101 VSDTAGLGSG RTAGFSAFAS GAATFASGFS TGFSTGFSIG FSTVACLDGS 151 DGMDAVSALG FAVCGLGCSA LIFLGAAALI LFRFGM* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1733>: m575.seq.. 1 ATGGTTTCGG GCGAGGAAGC CTTCAGGAAG CCTGCCAGTC CGGAGGGTGA 51 GGCAGGTTTT GCGGAAGCTG TTTCTTCTGT GCCGATATGG TTGTTTGAGG 101 GCAGGTTGTC GGAGAAATCG GTATCGACGG TTTCCGGTTT GTTTTCGGCA 151 GTTTGGGCGA CAGATTCCGG TTCGGGCGTG TCGATGACGA TTTCGACAGG 201 GTTGTACGGG TTGAAGGTCT CGGGCTCGTA CACGCTGTCT GTGGATTCGA 251 TGGCGTTCCA ATCGGCATCC GCGCGTTTTT GGGTTTCTTC ATCCTGCGTA 301 AGTGCGCCGG ATAAAATGCC GTTTTGCGCG GCTGCCAGGC TGTCGAAATC 351 CAAGTCGATG CGGTTGGAAG GCGTATCGGT TTCGACATCG AACGTTTGTT 401 TTGCCGATAA CTCTTCTTCA GATTCCCCAT CTAAGGCAAG TGTGTCGTTT 451 ACATCGTTTT TCGGAGCGGG TTCGGGCGTT GCCGGAGTTT CGACTTCGGC 501 AAAGGTGATT TCTATGCCGT CGTCTGCCGC GTCGTCAAGG TCAGGCTCTT 551 CCTCAGGGAC GGATTCTTCG GTACGGCGCG CGCGTTTGGA TTGGGCAAGG 601 CGCAAAAGCA GCAGCAGGGC GATTAATGCC GCGCCTCCGC CGGCAAGCAG 651 CAAGGTGTAC GAACCGCCGA ACAGACCGTC AAACAGTCCG CTTTCGGTTT 701 CTTCTTCGGC AGAAACCTGT TCGACAGGTT CGGAAACGGC GTTACCGGTT 751 TCGTCGGTCG GCGTGTCGAT GGCAGAAGCG GCGGCTTCTT GGGGGGCGGA 801 TTCGGCAGCG GTTTCCGATG CGGCAGTATT TGCAGCGGGT ACAGGTTCGG 851 GTCGAACGGC CGGTTTTTCC GCTTTTGCTT CGGGCGCGGC AACTTTTGCT 901 TCAGGTTTTT CAACCGGTTT CTCTACCGTT GCCTGTTTGG ACGGTTCGGA 951 CGGCATGGAT GCGGTTTCGG CTTTGGGTTT CGCCGTTTGC GGTTTGGGTT 1001 GTTCCGCTTT GATCCTGTTC AGATTCGGAA TGTGA This corresponds to the amino acid sequence <SEQ ID 1734; ORF 575>: m575.pep 1 MVSGEEAFRK PAΞPEGEAGF AEAVSSVPIW LFEGRLSEKS VSTVΞGLFΞA 51 VWATDSGSGV SMTISTGLYG LKVSGSYTLS VDSMAFQΞAS ARFWVSSSCV 101 SAPDKMPFCA AARLSKSKΞM RLEGVSVSTS NVCFADNSSS DSPSKAΞVSF 151 TSFFGAGSGV AGVSTSAKVI SMPΞSAASSR SGSSSGTDSS VRRARLDWAR 201 RKSSSRAINA APPPASSKVY EPPNRPSNSP LSVSSSAETC STGSETALPV 251 SSVGVSMAEA AASWGADSAA VSDAAVFAAG TGSGRTAGFS AFASGAATFA 301 SGFSTGFSTV ACLDGΞDGMD AVSALGFAVC GLGCSALILF RFGM* m575/g575 70.2% identity in 114 aa overlap 240 250 260 270 280 m575.pep SSAETCSTGSETALPVSSVGVSMAEAAASWGADSAAVSDAAVFAAGTG g575 LHWLFPQQVRKRCYRFRRSACRWQKRRLLGGADSAAVSDAAVFAAGTGPGWRSVAEAGVS 50 60 70 80 90 100 290 300 309 310 320 m575. pep SGRTAGFSAFASGAATFASGFSTGFΞT VACLDGSDGMDAVSALGFA l l l l l l l l l l l l l l l l l l l l l l l l l l l I I I I I I I I M I I I I M I I I g575 DTAGLGSGRTAGFSAFASGAATFASGFSTGFΞTGFSIGFSTVACLDGSDGMDAVSALGFA 110 120 130 140 150 160 330 340 m575. pep VCGLGCSALI LFRFGMX g575 VCGLGCSALIFLGAAALILFRFGMX 170 180 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1735>: a575. seq 1 ATGGTTTCGG GCGAGGAAGC CTTCAGGAAG CCTGCCAGTC CGGAGGGTGA 51 GGCAGGTTTT GCGGAAGCTG TTTCTTCTGT GCCGATATGG TTGTTTGAGG 101 GCAGGTTGTC GGAGAAATCG GTATCGACGG TTTCCGGTTT GTTTTCGGCA 151 GTTTGGGCGA CAGATTCCGG TTCGGGCGTG TCGATGACGA TTTCGACAGG 201 GTTGTACGGG TTGAAGGTCT CGGGCTCGTA CACGCTGTCT GTGGATTCGA 251 TGGCGTTCCA ATCGGCATCC GCGCGTTTTT GGGTTTCTTC ATCCTGCGTA 301 AGTGCGCCGG ATAAAATGCC GTTTTGCGCG GCTGCCAGGC TGTCGAAATC 351 CAAGTCGATG CGGTTGGAAG GCGTATCGGT TTCGACATCG AACGTTTGTT 401 TTGCCGACAA CTCTTCTTCA GATTCCCCAT CTAAGGCAAG TGTGTCGTTT 451 ACATCGTTTT TCGGAGCGGG TTCGGGCGTT GCCGGAGTTT CGACTTCGGC 501 AAAGGTGATT TCTATGCCGT CGTCTGCCGC GTCGTCAAGG TCAGGCTCTT 551 CCTCAGGGAC GGATTCTTCG GTACGGCGCG CGCGTTTGGA TTGGGCAAGG 601 CGCAAAAGCA GCAGCAGGGC GATCAATGCC GCGCCTCCGC CGGCAAGCAG 651 CAAGGTGTAC GAACCGCCGA ACAGTCCGCT TTCGGTTTCT TCTTCGGCAG 701 AAACCTGTTC GACAGGTTCG GAAACGGCGT TACCGGTTTC GTCGGTCGGC 751 GTGTCGATGG CAGAAGCGGC GGCTTCTTGG GGGGCGGATT CGGCAGCGGT 801 TTCCGATGCG GCAGTATTTG CAGCGGGTAC AGGTTCGGGT CGAACGGCCG 851 GTTTTTCCGC TTTTGCTTCG GGCGCGGCAA CTTTTGCTTC AGGTTTTTCA 901 ACCGGTTTCT CTACCGTTGC CTGTTTGGAC GGTTCGGACG GCATGGATGC 951 GGTTTCGGCT TTGGGTTTCG CCGTTTGCGG TTTGGGTTGT TCCGCTTTGA 1001 TCCTGTTCAG ATTCGGAATG TGA This corresponds to the amino acid sequence <SEQ ID 1736; ORF 575. a>: a575.pep 1 MVSGEEAFRK PASPEGEAGF AEAVSSVPIW LFEGRLSEKS VSTVSGLFSA 51 VWATDSGSGV SMTISTGLYG LKVSGSYTLS VDSMAFQSAS ARFWVSSSCV 101 SAPDKMPFCA AARLSKSKSM RLEGVSVSTS ΝVCFADΝSSS DSPSKASVSF 151 TSFFGAGSGV AGVSTSAKVI SMPSSAASSR SGSSSGTDSS VRRARLDWAR 201 RKSSSRAINA APPPASSKVY EPPNSPLSVS SSAETCSTGS ETALPVSSVG 251 VSMAEAAASW GADSAAVSDA AVFAAGTGSG RTAGFSAFAS GAATFASGFS 301 TGFSTVACLD GSDGMDAVSA LGFAVCGLGC SALILFRFGM * m575/a575 98.8% identity in 344 aa overlap 10 20 30 40 50 60 m575.pep MVSGEEAFRKPASPEGEAGFAEAVSSVPIWLFEGRLSEKSVSTVSGLFSAVWATDSGSGV I II I I I I II I II II II II II II I II I II II II II II II II II I I II I I II II I I I I II I I a575 MVSGEEAFRKPASPEGEAGFAEAVSSVPIWLFEGRLSEKSVSTVSGLFSAVWATDSGSGV 10 20 30 40 50 60 70 80 90 100 110 120 m575.pep SMTISTGLYGLKVSGSYTLSVDSMAFQSASARFWVSSSCVSAPDKMPFCAAARLSKSKSM I I I I I I I I I I II II II II II I II II II II I II II II II II I II II II I II II I I II II I I a575 SMTISTGLYGLKVSGSYTLSVDSMAFQSASARFWVSSSCVSAPDKMPFCAAARLSKSKSM 70 80 90 100 110 120 130 140 150 160 170 180 m575.pep RLEGVSVSTSNVCFADNSSSDSPSKASVSFTSFFGAGSGVAGVSTSAKVISMPSSAASSR I I I II II I II I II II II II I II I II I II II II II II II II II II II II II II II II II II a575 RLEGVSVSTSNVCFADNSSSDSPSKASVSFTSFFGAGSGVAGVSTSAKVISMPSSAASSR 130 140 150 160 170 180 190 200 210 220 230 240 m575. pep SGSSSGTDSSVRRARLDWARRKSSSRAINAAPPPASSKVYEPPNRPSNSPLSVSSSAETC I II I II II II II II I II II II I I I II II I I I II I I II I I II I II II II I I II I I I I a575 SGSSSGTDSSVRRARLDWARRKSSSRAINAAPPPASSKVYEPPN SPLSVSSSAETC 190 200 210 220 230 250 260 270 280 290 300 m575. pep STGSETALPVSSVGVSMAEAAASWGADSAAVSDAAVFAAGTGSGRTAGFSAFASGAATFA I I I II II I II I II II I II I I II II I I I II II II II II I II II II I II I II II II II II II a575 STGSETALPVSSVGVSMAEAAASWGADSAAVSDAAVFAAGTGSGRTAGFSAFASGAATFA 240 250 260 270 280 290 310 320 330 340 m575.pep SGFSTGFSTVACLDGSDGMDAVSALGFAVCGLGCSALILFRFGMX I I I II II I II II II I II II II II II II II II II II II II II II II a575 SGFSTGFSTVACLDGSDGMDAVSALGFAVCGLGCSALILFRFGMX 300 310 320 330 340 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1737>: g576.sβq.. (partial) 1 ..atgggcgtgg acatcggacg ctccctgaaa caaatgaagg aacagggcgc 51 ggaaatcgat ttgaaagtct ttaccgatgc catgcaggca gtgtatgacg 101 gcaaagaaat caaaatgacc gaagagcagg cccaggaagt gatgatgaaa 151 ttcctgcagg agcagcaggc taaagccgta gaaaaacaca aggcggatgc 201 gaaggccaac aaagaaaaag gcgaagcctt cctgaaggaa aatgccgccg 251 aagacggcgt gaagaccact gcttccggtc tgcagtacaa aatcaccaaa 301 cagggtgaag gcaaacagcc gacaaaagac gacatcgtta ccgtggaata 351 cgaaggccgc ctgattgacg gtaccgtatt cgacagcagc aaagccaacg 401 gcggcccggc caccttccct ttgagccaag tgattccggg ttggaccgaa 451 ggcgtacggc ttctgaaaga aggcggcgaa gccacgttct acatcccgtc 501 caaccttgcc taccgcgaac agggtgcggg cgaaaaaatc ggtccgaacg 551 ccactttggt atttgacgtg aaactggtca aaatcggcgc acccgaaaac 601 gcgcccgcca agcagccgga tcaagtcgac atcaaaaaag taaattaa This corresponds to the amino acid sequence <SEQ ID 1738; ORF 576.ng>: g576.pep.. (partial) 1 ..MGVDIGRSLK QMKEQGAEID LKVFTDAMQA VYDGKEIKMT EEQAQEVMMK 51 FLQEQQAKAV EKHKADAKAΝ KEKGEAFLKE ΝAAEDGVKTT ASGLQYKITK 101 QGEGKQPTKD DIVTVEYEGR LIDGTVFDSS KAΝGGPATFP LSQVIPGWTE 151 GVRLLKEGGE ATFYIPSΝLA YREQGAGEKI GPΝATLVFDV KLVKIGAPEΝ 201 APAKQPDQVD IKKVΝ* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1739>: m576.seq.. (partial) 1 ..ATGCAGCAGG CAAGCTATGC GATGGGCGTG GACATCGGAC GCTCCCTGAA 51 GCAAATGAAG GAACAGGGCG CGGAAATCGA TTTGAAAGTC TTTACCGAAG 101 CCATGCAGGC AGTGTATGAC GGCAAAGAAA TCAAAATGAC CGAAGAGCAG 151 GCTCAGGAAG TCATGATGAA ATTCCTTCAG GAACAACAGG CTAAAGCCGT 201 AGAAAAACAC AAGGCGGACG CGAAGGCCAA TAAAGAAAAA GGCGAAGCCT 251 TTCTGAAAGA AAATGCCGCC AAAGACGGCG TGAAGACCAC TGCTTCCGGC 301 CTGCAATACA AAATCACCAA ACAGGGCGAA GGCAAACAGC CGACCAAAGA 351 CGACATCGTT ACCGTGGAAT ACGAAGGCCG CCTGATTGAC GGTACGGTAT 401 TCGACAGCAG CAAAGCCAAC GGCGGCCCGG TCACCTTCCC TTTGAGCCAA 451 GTGATTCCGG GTTGGACCGA AGgCGTACAG CTTCTGAAAG AAGGCGGCGA 501 AGCCACGTTC TACATCCCGT CCAACCTTGC CTACCGCGAA CAGGGTGCGG 551 GCGACAAAAT CGGTCCGAAC GCCACTTTGG TATTTGATGT GAAACTGGTC 601 AAAATCGGCG CACCCGAAAA CGCGCCCGCC AAGCAGCCGG CTCAAGTCGA 651 CATCAAAAAA GTAAATTAA This corresponds to the amino acid sequence <SEQ ID 1740; ORF 576>: m576.pep.. (partial) 1 ..MQQASYAMGV DIGRSLKQMK EQGAEIDLKV FTEAMQAVYD GKEIKMTEEQ 51 AQEVMMKFLQ EQQAKAVEKH KADAKANKEK GEAFLKENAA KDGVKTTASG 101 LQYKITKQGE GKQPTKDDIV TVEYEGRLID GTVFDSSKAN GGPVTFPLSQ 151 VIPGWTEGVQ LLKEGGEATF YIPSNLAYRE QGAGDKIGPN ATLVFDVKLV 201 KIGAPENAPA KQPAQVDIKK VN* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m576/g576 97.2% identity in 215 aa overlap 10 20 30 40 50 60 m576. pep MQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQEVMMKFLQ II II I I I II II II II II II II II II: II II I II II II II II II II II II II II g576 MGVDIGRSLKQMKEQGAEIDLKVFTDAMQAVYDGKEIKMTEEQAQEVMMKFLQ 10 20 30 40 50 70 80 90 100 110 120 m576. pep EQQAKAVEKHKADAKAΝKEKGEAFLKEΝAAKDGVKTTASGLQYKITKQGEGKQPTKDDIV II II II II II II II II II II II II II I II I: II II II II II I II II II II II II II II II g576 EQQAKAVEKHKADAKAΝKEKGEAFLKEΝAAEDGVKTTASGLQYKITKQGEGKQPTKDDIV 60 70 80 90 100 110 130 140 150 160 170 180 m576.pep TVEYEGRLIDGTVFDSSKAΝGGPVTFPLSQVIPGWTEGVQLLKEGGEATFYIPSΝLAYRE II II I II I I I II II II I II II II : II II II I II I II II I : II II II II II II II II II I I g576 TVEYEGRLIDGTVFDSSKAΝGGPATFPLSQVIPGWTEGVRLLKEGGEATFYIPSΝLAYRE 120 130 140 150 160 170 190 200 210 220 m576. pep QGAGDKIGPΝATLVFDVKLVKIGAPEΝAPAKQPAQVDIKKVΝX II II : II II II I II II II II II II II II II I II lllllllll g576 QGAGEKIGPΝATLVFDVKLVKIGAPEΝAPAKQPDQVDIKKVΝX 180 190 200 210 The following partial DΝA sequence was identified inN meningitidis <SEQ ID 1741>: a576. seq 1 ATGAACACCA TTTTCAAAAT CAGCGCACTG ACCCTTTCCG CCGCTTTGGC 51 ACTTTCCGCC TGCGGCAAAA AAGAAGCCGC CCCCGCATCT GCATCCGAAC 101 CTGCCGCCGC TTCTTCCGCG CAGGGCGACA CCTCTTCGAT CGGCAGCACG 151 ATGCAGCAGG CAAGCTATGC GATGGGCGTG GACATCGGAC GCTCCCTGAA 201 GCAAATGAAG GAACAGGGCG CGGAAATCGA TTTGAAAGTC TTTACCGAAG 251 CCATGCAGGC AGTGTATGAC GGCAAAGAAA TCAAAATGAC CGAAGAGCAG 301 GCTCAGGAAG TCATGATGAA ATTCCTTCAG GAACAACAGG CTAAAGCCGT 351 AGAAAAACAC AAGGCGGACG CGAAGGCCAA TAAAGAAAAA GGCGAAGCCT 401 TTCTGAAAGA AAATGCCGCC AAAGACGGCG TGAAGACCAC TGCTTCCGGC 451 CTGCAATACA AAATCACCAA ACAGGGCGAA GGCAAACAGC CGACCAAAGA 501 CGACATCGTT ACCGTGGAAT ACGAAGGCCG CCTGATTGAC GGTACGGTAT 551 TCGACAGCAG CAAAGCCAAC GGCGGCCCGG TCACCTTCCC TTTGAGCCAA 601 GTGATTCTGG GTTGGACCGA AGGCGTACAG CTTCTGAAAG AAGGCGGCGA 651 AGCCACGTTC TACATCCCGT CCAACCTTGC CTACCGCGAA CAGGGTGCGG 701 GCGACAAAAT CGGCCCGAAC GCCACTTTGG TATTTGATGT GAAACTGGTC 751 AAAATCGGCG CACCCGAAAA CGCGCCCGCC AAGCAGCCGG CTCAAGTCGA 801 CATCAAAAAA GTAAATTAA This corresponds to the amino acid sequence <SEQ ID 1742; ORF 576.a>: a576.pep 1 MNTIFKISAL TLSAALALSA CGKKEAAPAS ASEPAAASSA QGDTSSIGST 51 MQQASYAMGV DIGRSLKQMK EQGAEIDLKV FTEAMQAVYD GKEIKMTEEQ 101 AQEVMMKFLQ EQQAKAVEKH KADAKANKEK GEAFLKENAA KDGVKTTASG 151 LQYKITKQGE GKQPTKDDIV TVEYEGRLID GTVFDSSKAN GGPVTFPLSQ 201 VILGWTEGVQ LLKEGGEATF YIPSNLAYRE QGAGDKIGPN ATLVFDVKLV 251 KIGAPENAPA KQPAQVDIKK VN* m576/a576 99.5% identity in 222 aa overlap 10 20 30 m576. pep MQQASYAMGVDIGRSLKQMKEQGAEIDLKV I II II II I II II II II I I II II I I II I I I I a576 CGKKEAAPASAΞEPAAASSAQGDTSSIGSTMQQASYAMGVDIGRSLKQMKEQGAEIDLKV 30 40 50 60 70 80 40 50 60 70 80 90 m576. pep FTEAMQAVYDGKEIKMTEEQAQEVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAA II I II II II II II I II II II II II II II II II II II II II I II II II II I I II II II I II a576 FTEAMQAVYDGKEIKMTEEQAQEVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAA 90 100 110 120 130 140 100 110 120 130 140 150 m576. pep KDGVKTTASGLQYKITKQGEGKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQ II I II I II II I II I I II II II II I II II II II II II II II II II I II I II II II II II II a576 KDGVKTTASGLQYKITKQGEGKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQ 150 160 170 180 190 200 160 170 180 190 200 210 m576. pep VIPGWTEGVQLLKEGGEATFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPA II I II II II II I II II II I I II I II II II II II I II I II II II II II II II II I II I II a576 VILGWTEGVQLLKEGGEATFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPA 210 220 230 240 250 260 220 m576.pep KQPAQVDIKKVNX II II II II II I II a576 KQPAQVDIKKVNX 270 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1743>: g576-l.seq 1 ATGAACACCA TTTTCAAAAT CAGCGCACTG ACCCTTTCCG CCGCTTTGGC 51 ACTTTCCGCC TGCGGCAAAA AAGAAGCCGC CCCCGCATCT GCATCCGAAC 101 CTGCCGCCGC TTCTGCCGCG CAGGGCGACA CCTCTTCAAT CGGCAGCACG 151 ATGCAGCAGG CAAGCTATGC AATGGGCGTG GACATCGGAC GCTCCCTGAA 201 ACAAATGAAG GAACAGGGCG CGGAAATCGA TTTGAAAGTC TTTACCGATG 251 CCATGCAGGC AGTGTATGAC GGCAAAGAAA TCAAAATGAC CGAAGAGCAG 301 GCCCAGGAAG TGATGATGAA ATTCCTGCAG GAGCAGCAGG CTAAAGCCGT 351 AGAAAAACAC AAGGCGGATG CGAAGGCCAA CAAAGAAAAA GGCGAAGCCT 401 TCCTGAAGGA AAATGCCGCC AAAGACGGCG TGAAGACCAC TGCTTCCGGT 451 CTGCAGTACA AAATCACCAA ACAGGGTGAA GGCAAACAGC CGACAAAAGA 501 CGACATCGTT ACCGTGGAAT ACGAAGGCCG CCTGATTGAC GGTACCGTAT 551 TCGACAGCAG CAAAGCCAAC GGCGGCCCGG CCACCTTCCC TTTGAGCCAA 601 GTGATTCCGG GTTGGACCGA AGGCGTACGG CTTCTGAAAG AAGGCGGCGA 651 AGCCACGTTC TACATCCCGT CCAACCTTGC CTACCGCGAA CAGGGTGCGG 701 GCGAAAAAAT CGGTCCGAAC GCCACTTTGG TATTTGACGT GAAACTGGTC 751 AAAATCGGCG CACCCGAAAA CGCGCCCGCC AAGCAGCCGG ATCAAGTCGA 801 CATCAAAAAA GTAAATTAA This corresponds to the amino acid sequence <SEQ ID 1744; ORF 576-l.ng>: g576-l.pep 1 MNTIFKISAL TLSAALALSA CGKKEAAPAS ASEPAAAΞAA QGDTSSIGST 51 MQQASYAMGV DIGRSLKQMK EQGAEIDLKV FTDAMQAVYD GKEIKMTEEQ 101 AQEVMMKFLQ EQQAKAVEKH KADAKANKEK GEAFLKENAA KDGVKTTASG 151 LQYKITKQGE GKQPTKDDIV TVEYEGRLID GTVFDSSKAN GGPATFPLΞQ 201 VIPGWTEGVR LLKEGGEATF YIPSNLAYRE QGAGEKIGPN ATLVFDVKLV 251 KIGAPENAPA KQPDQVDIKK VN* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1745>: m576-l.seq 1 ATGAACACCA TTTTCAAAAT CAGCGCACTG ACCCTTTCCG CCGCTTTGGC 51 ACTTTCCGCC TGCGGCAAAA AAGAAGCCGC CCCCGCATCT GCATCCGAAC 101 CTGCCGCCGC TTCTTCCGCG CAGGGCGACA CCTCTTCGAT CGGCAGCACG 151 ATGCAGCAGG CAAGCTATGC GATGGGCGTG GACATCGGAC GCTCCCTGAA 201 GCAAATGAAG GAACAGGGCG CGGAAATCGA TTTGAAAGTC TTTACCGAAG 251 CCATGCAGGC AGTGTATGAC GGCAAAGAAA TCAAAATGAC CGAAGAGCAG 301 GCTCAGGAAG TCATGATGAA ATTCCTTCAG GAACAACAGG CTAAAGCCGT 351 AGAAAAACAC AAGGCGGACG CGAAGGCCAA TAAAGAAAAA GGCGAAGCCT 401 TTCTGAAAGA AAATGCCGCC AAAGACGGCG TGAAGACCAC TGCTTCCGGC 451 CTGCAATACA AAATCACCAA ACAGGGCGAA GGCAAACAGC CGACCAAAGA 501 CGACATCGTT ACCGTGGAAT ACGAAGGCCG CCTGATTGAC GGTACGGTAT 551 TCGACAGCAG CAAAGCCAAC GGCGGCCCGG TCACCTTCCC TTTGAGCCAA 601 GTGATTCCGG GTTGGACCGA AGGCGTACAG CTTCTGAAAG AAGGCGGCGA 651 AGCCACGTTC TACATCCCGT CCAACCTTGC CTACCGCGAA CAGGGTGCGG 701 GCGACAAAAT CGGTCCGAAC GCCACTTTGG TATTTGATGT GAAACTGGTC 751 AAAATCGGCG CACCCGAAAA CGCGCCCGCC AAGCAGCCGG CTCAAGTCGA 801 CATCAAAAAA GTAAATTAA This corresponds to the amino acid sequence <SEQ ID 1746; ORF 576-1 > • m576-l .pep 1 MΝTIFKISAL TLSAALALSA CGKKEAAPAS ASEPAAASSA QGDTSSIGST 51 MQQASYAMGV DIGRSLKQMK EQGAEIDLKV FTEAMQAVYD GKEIKMTEEQ 101 AQEVMMKFLQ EQQAKAVEKH KADAKAΝKEK GEAFLKEΝAA KDGVKTTASG 151 LQYKITKQGE GKQPTKDDIV TVEYEGRLID GTVFDSSKAN GGPVTFPLSQ 201 VIPGWTEGVQ LLKEGGEATF YIPSNLAYRE QGAGDKIGPN ATLVFDVKLV 251 KIGAPENAPA KQPAQVDIKK VN* g576-l/m576-l 97.8% identity in 272 aa overlap 10 20 30 40 50 60 g576-l.pep MNTIFKISALTLSAALALΞACGKKEAAPAΞASEPAAASAAQGDTSSIGSTMQQASYAMGV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I m576-l MNTIFKISALTLSAALALSACGKKEAAPAΞASEPAAASSAQGDTSSIGΞTMQQASYAMGV 10 20 30 40 50 60 70 80 90 100 110 120 g576-l.pep DIGRΞLKQMKEQGAEIDLKVFTDAMQAVYDGKEIKMTEEQAQEVMMKFLQEQQAKAVEKH I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m576-l DIGRΞLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQEVMMKFLQEQQAKAVEKH 70 80 90 100 110 120 130 140 150 160 170 180 g576-l.pep KADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGEGKQPTKDDIVTVEYEGRLID I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m5 6-l KADAKANKEKGEAFLKENAAKDGVKTTAΞGLQYKITKQGEGKQPTKDDIVTVEYEGRLID 130 140 150 160 170 180 190 200 210 220 230 240 g576-l.pep GTVFDSSKANGGPATFPLSQVIPGWTEGVRLLKEGGEATFYIPSNLAYREQGAGEKIGPN I I I I I I I I I I I I I : I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I m576-l GTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEATFYIPSNLAYREQGAGDKIGPN 190 200 210 220 230 240 250 260 270 g576-l -pep ATLVFDVKLVKIGAPENAPAKQPDQVDIKKVNX I I I I I I I I I I I I I I I I I I I I I I I m576-l ATLVFDVKLVKIGAPENAPAKQPAQVDIKKVNX 250 260 270 The following partial DNA sequence was identified inN meningitidis <SEQ ID 1747>: a576-l . seq 1 ATGAACACCA TTTTCAAAAT CAGCGCACTG ACCCTTTCCG CCGCTTTGGC 51 ACTTTCCGCC TGCGGCAAAA AAGAAGCCGC CCCCGCATCT GCATCCGAAC 101 CTGCCGCCGC TTCTTCCGCG CAGGGCGACA CCTCTTCGAT CGGCAGCACG 151 ATGCAGCAGG CAAGCTATGC GATGGGCGTG GACATCGGAC GCTCCCTGAA 201 GCAAATGAAG GAACAGGGCG CGGAAATCGA TTTGAAAGTC TTTACCGAAG 251 CCATGCAGGC AGTGTATGAC GGCAAAGAAA TCAAAATGAC CGAAGAGCAG 301 GCTCAGGAAG TCATGATGAA ATTCCTTCAG GAACAACAGG CTAAAGCCGT 351 AGAAAAACAC AAGGCGGACG CGAAGGCCAA TAAAGAAAAA GGCGAAGCCT 401 TTCTGAAAGA AAATGCCGCC AAAGACGGCG TGAAGACCAC TGCTTCCGGC 451 CTGCAATACA AAATCACCAA ACAGGGCGAA GGCAAACAGC CGACCAAAGA 501 CGACATCGTT ACCGTGGAAT ACGAAGGCCG CCTGATTGAC GGTACGGTAT 551 TCGACAGCAG CAAAGCCAAC GGCGGCCCGG TCACCTTCCC TTTGAGCCAA 601 GTGATTCTGG GTTGGACCGA AGGCGTACAG CTTCTGAAAG AAGGCGGCGA 651 AGCCACGTTC TACATCCCGT CCAACCTTGC CTACCGCGAA CAGGGTGCGG 701 GCGACAAAAT CGGCCCGAAC GCCACTTTGG TATTTGATGT GAAACTGGTC 751 AAAATCGGCG CACCCGAAAA CGCGCCCGCC AAGCAGCCGG CTCAAGTCGA 801 CATCAAAAAA GTAAATTAA This corresponds to the amino acid sequence <SEQ ID 1748; ORF 576-1. a>: a576-l.pep 1 MNTIFKISAL TLSAALALSA CGKKEAAPAS ASEPAAASSA QGDTSSIGST 51 MQQASYAMGV DIGRSLKQMK EQGAEIDLKV FTEAMQAVYD GKEIKMTEEQ 101 AQEVMMKFLQ EQQAKAVEKH KADAKANKEK GEAFLKENAA KDGVKTTASG 151 LQYKITKQGE GKQPTKDDIV TVEYEGRLID GTVFDSSKAN GGPVTFPLSQ 201 VILGWTEGVQ LLKEGGEATF YIPSNLAYRE QGAGDKIGPN ATLVFDVKLV 251 KIGAPENAPA KQPAQVDIKK VN* a576-l/ 576-l 99.6% identity in 272 aa overlap 10 20 30 40 50 60 a576-l.pep MNTIFKISALTLΞAALALSACGKKEAAPAΞAΞEPAAASΞAQGDTSSIGSTMQQASYAMGV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m576-l MNTIFKIΞALTLSAALALΞACGKKEAAPASASEPAAASSAQGDTSΞIGSTMQQASYAMGV 10 20 30 40 50 60 70 80 90 100 110 120 a576-l.pep DIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQEVMMKFLQEQQAKAVEKH I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m576-1 DIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQEVMMKFLQEQQAKAVEKH 70 80 90 100 110 120 130 140 150 160 170 180 a576-l.pep KADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGEGKQPTKDDIVTVEYEGRLID I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m576-l KADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGEGKQPTKDDIVTVEYEGRLID 130 140 150 160 170 180 190 200 210 220 230 240 a576-l.pep GTVFDSSKANGGPVTFPLΞQVILGWTEGVQLLKEGGEATFYIPSNLAYREQGAGDKIGPN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I πι576-l GTVFDΞSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEATFYIPSNLAYREQGAGDKIGPN 190 200 210 220 230 240 250 260 270 a576-l .pep ATLVFDVKLVKIGAPENAPAKQPAQVDIKKVNX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m576-1 ATLVFDVKLVKIGAPENAPAKQPAQVDIKKVNX 250 260 270 Expression of ORF 576 The primer described in Table 1 for ORF 576 was used to locate and clone ORF 576. ORF 576 was cloned in pET and pGex vectors and expressed in E.coli as above described. The products of protein expression and purification were analyzed by SDS-PAGE. Figure 3 A shows the results of affinity purification and Figure 3B shows the expression in E.coli. Purified His-fusion protein was used to immunize mice, whose sera were used for ELISA (positive result), FACS analysis (Figure 3C), western blot (Figure 3D). These experiments confirm that ORF 576 is a surface-exposed protein and that it is a useful immunogen. The hydrophilicity plots, antigenic index, and amphipatic regions of ORF 576 are provided in Figure 7. The AMPHI program is used to predict putative T-cell epitopes (Gao et al 1989, J. Immunol 143:3007; Roberts et al. 1996, AIDS Res Human Retroviruses 12:593; Quakyi et al. 1992, Scand J Immunol Suppl 11:9). The nucleic acid sequence of ORF 576 and the amino acid sequence encoded thereby is provided in Example 1. The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1749>: g57 . seq.. 1 atggaaagga gcggtgtatt tggtaaaatt gtcggcaatc gcatactccg 51 tatgccgtcc gaacacgctg ccgcattcta tccgaaaccg tgcaaatcgt 101 ttaaactaac gcaatcttgg ttcagagtgc gaagctgtcc gtgcggcgtt 151 tttatttacg gagcaaacat gaaacttatc tataccgtca tcaaaatcat 201 tatcctgctg ctcttcctgc tgcttgccgt cattaatatg gatgccgtta 251 ccttttccta tcttccgggg cagagtgtca atctgccgct gattgtcgta 301 ttgttcggcg cgtttgtcgt cggcatcgtg ttcggaatgt ttgccctgtt 351 cgggcggctg ctgtccttgc gcggcgaaaa cagccgcctg cgtgcggaag 401 tgaagaaaag tgcgcgcttg agcggacaga aattgactgc accgccgata 451 caaaatgctg ccgaatctgc caaacagcct taa This corresponds to the amino acid sequence <SEQ ID 1750; ORF 577.ng>: g577.pep 1 MERSGVFGKI VGΝRILRMPS EHAAAFYPKP CKSFKLTQSW FRVRSCPCGV 51 FIYGAΝMKLI YTVIKIIILL LFLLLAVIΝM DAVTFSYLPG QSVΝLPLIW 101 LFGAFWGIV FGMFALFGRL LSLRGEΝSRL RAEVKKSARL SGQKLTAPPI 151 QΝAAESAKQP * The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1751>: m577. seq.. 1 ATGGAAAGGA ACGGTGTATT TGGTAAAATT GTCGGCAATC GCATACTCCG 51 TATGTCGTCC GAACACGCTG CCGCATCCTA TCCGAAACCG TGCAAATCGT 101 TTAAACTAGC GCAATCTTGG TTCAGAGTGC GAAGCTGTCT GGGCGGCGTT 151 TTTATTTACG GAGCAAACAT GAAACTTATC TATACCGTCA TCAAAATCAT 201 TATCCTGCTG CTCTTCCTGC TGCTTGCCGT CATTAATACG GATGCCGTTA 251 CCTTTTCCTA CCTGCCGGGG CAAAAATTCG ATTTGCCGCT GATTGTCGTA 301 TTGTTCGGCG CATTTGTAGT CGGTATTATT TTTGGAATGT TTGCCTTGTT 351 CGGACGGTTG TTGTCGTTAC GTGGCGAGAA CGGCAGGTTG CGTGCCGAAG 401 TAAAGAAAAA TGCGCGTTTG ACGGGGAAGG AGCTGACCGC ACCACCGGCG 451 CAAAATGCGC CCGAATCTAC CAAACAGCCT TAA This corresponds to the amino acid sequence <SEQ ID 1752; ORF 577>: m577.pep.. 1 MERΝGVFGKI VGΝRILRMSS EHAAASYPKP CKSFKLAQSW FRVRSCLGGV 51 FIYGAΝMKLI YTVIKIIILL LFLLLAVIΝT DAVTFSYLPG QKFDLPLIW 101 LFGAFWGII FGMFALFGRL LSLRGEΝGRL RAEVKKΝARL TGKELTAPPA 151 QΝAPESTKQP Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N. gonorrhoeae m577/g577 88.1% identity 160 aa overlap 10 20 30 40 50 60 m577. pep MERΝGVFGKIVGΝRILRMSSEHAAASYPKPCKSFKLAQSWFRVRSCLGGVFIYGAΝMKLI M M M M M M M M l l l l l l I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I g577 MERSGVFGKIVGΝRILRMPSEHAAAFYPKPCKSFKLTQSWFRVRSCPCGVFIYGAΝMKLI 10 20 30 40 50 60 70 80 90 100 110 120 m577. pep YTVIKIIILLLFLLLAVIΝTDAVTFSYLPGQKFDLPLIWLFGAFWGIIFGMFALFGRL I I I I II II II II II I II II I II II II I II I : : I II II I II II II I II : II II I II II I g 577 YTVIKIIILLLFLLLAVINMDAVTFSYLPGQSVNLPLIWLFGAFWGIVFGMFALFGRL 70 80 90 100 110 120 130 140 150 160 m577.pep LSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESTKQPX I I I I II I : I II II II I : I I I : I : : II I II III II : I II I g577 LSLRGENSRLRAEVKKSARLSGQKLTAPPIQNAAESAKQPX 130 140 150 160 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1753>: a577. seq 1 ATGGAAAGGA ACGGTGTATT TGGTAAAATT GTCGGCAATC GCATACTCCG 51 TATGTCGTCC GAACACGCTG CCGCATCCTA TCCGAAACCG TGCAAATCGT 101 TTAAACTAGC GCAATCTTGG TTCAGAGTGC GAAGCTGTCC GGGCGGCGTT 151 TTTATTTACG GAGCAAACAT GAAACTTATC TATACCGTCA TCAAAATCAT 201 TATCCTGCTG CTCTTCCTGC TGCTTGCTGT CATTAATACG GATGCCGTTA 251 CCTTTTCCTA CCTGCCGGGG CAAAAATTCG ATTTGCCGCT GATTGTCGTA 301 TTGTTCGGCG CGTTTGTCGT CGGCATCGTG TTCGGAATGT TTGCCTTGTT 351 CGGACGGTTG TTGTCGTTAC GTGGCGAGAA CGGCAGGTTG CGTGCCGAAG 401 TAAAGAAAAA TGCGCGTTTG ACGGGGAAGG AGCTGACCGC ACCACCGGCG 451 CAAAATGCGC CCGAATCTGC CAAACAGCCT TGA This corresponds to the amino acid sequence <SEQ ID 1754; ORF 577. a>: a577.pep 1 MERΝGVFGKI VGΝRILRMSS EHAAASYPKP CKSFKLAQSW FRVRSCPGGV 51 FIYGAΝMKLI YTVIKIIILL LFLLLAVIΝT DAVTFSYLPG QKFDLPLIW 101 LFGAFWGIV FGMFALFGRL LSLRGEΝGRL RAEVKKΝARL TGKELTAPPA 151 QΝAPESAKQP * m577/a577 98.1% identity in 160 aa overlap 10 20 30 40 50 60 m577. pep MERΝGVFGKIVGΝRILRMSSEHAAASYPKPCKSFKLAQSWFRVRSCLGGVFIYGAΝMKLI II II II II II II II II II I II I II II II I II II II I II I I II II II lllllllllllll a577 MERΝGVFGKIVGΝRILRMSSEHAAASYPKPCKSFKLAQSWFRVRSCPGGVFIYGAΝMKLI 10 20 30 40 50 60 70 80 90 100 110 120 m577 . pep YTVIKI I ILLLFLLLAVIΝTDAVTFSYLPGQKFDLPLIWLFGAFWGI I FGMFALFGRL M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M a577 YTVIKI I ILLLFLLLAVIΝTDAVTFSYLPGQKFDLPLIWLFGAFWGIVFGMFALFGRL 70 80 90 100 110 120 130 140 150 160 m577 . pep LSLRGEΝGRLRAEVKKΝARLTGKELTAPPAQΝAPESTKQPX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I a 577 L S LRGEΝGRLRAEVKKΝARLTGKELT AP PAQΝAPE S AKQPX 130 140 150 160 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 1755>: g578.seq.. 1 atgggaaagc tcgacatcgg gatattgttt gccgatttct tcaaagattt 51 cgcgccacag ttcggtggtt tccaaaacgt tggctttgcc tacggagcag 101 acttttttgc tgcgtttttg ggcggattgg aaggccacgt gggcgatgcg 151 gcggatttcg ctttcgctgt atttcatggt gttgtagcct tcgtgttcgc 201 cgttttccaa aacacggatg ccgcgcggtt cgccgaaata aatatcgccg 251 gtaagttcgc gcacaatcaa aatatccaaa ccggcaacga tttcaggctt 301 gagcgtggag gcgttggcta a This corresponds to the amino acid sequence <SEQ ID 1756; ORF 578.ng>: g578.pep 1 MGKLDIGILF ADFFKDFAPQ FGGFQΝVGFA YGADFFAAFL GGLEGHVGDA 51 ADFAFAVFHG WAFVFAVFQ ΝTDAARFAEI ΝIAGKFAHΝQ ΝIQTGΝDFRL 101 ERGGVG* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1757>: m578.seq.. 1 ATGGGAAAGC TCGACATCAG GGTACTCTTT GCCGATTTCT TCAAAGATTT 51 CGCGCCACAA TTCGGTGGTT TCCAAAACGT TGGCTTTGCC TACGGAACAG 101 ACTTTTTTGC TGCGTTTTTG GGCGGATTGG AAGGCAACAT GGGCAATACG 151 GCGGATTTCG CTTTCGCTGT ATTTCATGGT GTTGTAGCCT TCGCGTTCGC 201 CGTTTTCCAG AACGCGGATG CCGCGCGGTT CGCCGAAATA GATGTCGCCG 251 GTGAGTTCGC GCACAATCAA AATATCCAAA CCGGCAACGA TTTCAGGCTT This corresponds to the amino acid sequence <SEQ ID 1758; ORF 578>: m578.pep.. 1 MGKLDIRVLF ADFFKDFAPQ FGGFQΝVGFA YGTDFFAAFL GGLEGΝMGΝT 51 ADFAFAVFHG WAFAFAVFQ ΝADAARFAEI DVAGEFAHΝQ ΝIQTGΝDFRL 101 QRGGVG* m578/g578 87.7% identity in 106 aa overlap 10 20 30 40 50 60 m578.pep MGKLDIRVLFADFFKDFAPQFGGFQΝVGFAYGTDFFAAFLGGLEGΝMGΝTADFAFAVFHG g578 MGKLDIGILFADFFKDFAPQFGGFQΝVGFAYGADFFAAFLGGLEGHVGDAADFAFAVFHG 10 20 30 40 50 60 70 80 90 100 m578.pep WAFAFAVFQΝADAARFAEIDVAGEFAHΝQΝIQTGΝDFRLQRGGVGX g578 WAFVFAVFQΝTDAARFAEIΝIAGKFAHΝQΝIQTGΝDFRLERGGVGX 70 80 90 100 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1759>: a578. seq 1 ATGGGAAAGC TCGACATCAG GGTATTCTTT GCCGATTTCT TCAAAGATTT 51 CGCGCCACAA TTCGGTGGTT TCCAAAACGT TGGCTTTGCC TACGGAGCAG 101 ACTTTTTTGC TGCGTTTTTG GGCGGATTGG AAGGCGACGT GGGCAATACG 151 GCGGATTTCG CTTTCGCTGT ATTTCATGGT GTTGTAGCCT TCGCGTTCGC 201 CGTTTTCCAG AACACGGATG CCGCGCGGTT CGCCGAAATA AATATCGCCG 251 GTGAGTTCGC GCACAATCAA AATATCCAAA CCCGCAACGA TTTCAGACTT 301 GAGCGTGGAG GCGTTGGCTA G This corresponds to the amino acid sequence <SEQ ID 1760; ORF 578.a>: a578.pep 1 MGKLDIRVFF ADFFKDFAPQ FGGFQΝVGFA YGADFFAAFL GGLEGDVGΝT 51 ADFAFAVFHG WAFAFAVFQ ΝTDAARFAEI ΝIAGEFAHΝQ ΝIQTRΝDFRL 101 ERGGVG* m578/a578 91.5% identity in 106 aa overlap 10 20 30 40 50 60 m578 . pep MGKLDIRVLFADFFKDFAPQFGGFQΝVGFAYGTDFFAAFLGGLEGΝMGΝTADFAFAVFHG M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M a578 MGKLDIRVFFADFFKDFAPQFGGFQΝVGFAYGADFFAAFLGGLEGDVGΝTADFAFAVFHG 10 20 30 40 50 60 70 80 90 100 m578. pep WAFAFAVFQΝADAARFAEIDVAGEFAHΝQΝIQTGΝDFRLQRGGVGX II II II II II I : I I II I II I : : I II II II II I I I II I II : I II II I a578 WAFAFAVFQΝTDAARFAEIΝIAGEFAHΝQΝIQTRΝDFRLERGGVGX 70 80 90 100 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 1761>: g579. seq.. 1 ATGAGGGCGG CGATGACGCG CGCGCAGGTC GATGCCACGC TGATTAGTTT 51 TTTGTGTAAT GTTGCCAATA TCGGCTTATT GATTTTGGTG ATTATTGCCG 101 CATTGGGACG GTTGGGCGTT TCCACAACAT CCGTAACCGC CTTAATCGGC 151 GGCGCGGGTT TGGCGGTGGC GTTGTCCTTA AAAGACCAGC TGTCCAATTT 201 TGCCGCCGGC GCGCTGATTA TCCTGTTCCG CCCGTTCAAA GTCGGCGACT 251 TTATCCGTGT CGGCGGTTTT GAAGGATATG TCCGGGAAAT CAAAATGGTG 301 CAGACTTCTT TGCGGACGAC CGACAACGAA GAAGTCGTGC TGCCCAACAG 351 CGTGGTGATG GGCAACAGCA TCGTCAACCG TTCCAGCCTG CCGCTTTGCC 401 GCGCCCAAGT GATAGTCGGC GTCGATTACA ACTGCGATTT GAAAGTGGCG 451 AAAGAGGCGG TGTTGAAAGC CGCCGCCGAA CACCCCTTGA GCGTTCAAAA 501 CGAAGAGCGG CAGCCCGCCG CCTACATCAC CGCCTTGGGC GACAATGCCA 551 TCGAAATCAC ATTATGGGCT TGGGCAAACG AAGCAGACCG CTGGACGCTG 601 CAATGCGACT TGAACGAACA AGTGGTCGAA AACCTCCGCA AAGTCAATAT 651 CAACATCCCG TTCCCGCAAC GCGACATACA CATCATCAAT TCTTAA This corresponds to the amino acid sequence <SEQ ID 1762; ORF 579.ng>: g579.pep.. 1 MRAAMTRAQV DATLISFLCN VANIGLLILV IIAALGRLGV STTSVTALIG 51 GAGLAVALSL KDQLSNFAAG ALIILFRPFK VGDFIRVGGF EGYVREIKMV 101 QTSLRTTDNE EWLPNSWM GNSIVNRSSL PLCRAQVIVG VDYNCDLKVA 151 KEAVLKAAAE HPLSVQNEER QPAAYITALG DNAIEITLWA WANEADRWTL 201 QCDLNEQWE NLRKVNINIP FPQRDIHIIN S* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1763>: m579. seq.. 1 ATGAGGGCGG CGATGACGCG CGCGCAGGTC GATGCCACGC TGATTAGTTT 51 TTTGTGTAAT GTTGCCAATA TCGGCTTATT GATTTTGGTG ATTATTGCCG 101 CATTGGGCAG ATTGGGCGTT TCCACAACAT CCGTAACCGC CTTAATCGGC 151 GGCGCGGGTT TGGCGGTGGC GTTGTCCCTG AAAGACCAGC TGTCCAATTT 201 TGCCGCCGGC GCACTGATTA TCCTGTTCCG CCCGTTCAAA GTCGGCGATT 251 TTATCCGCGT CGGCGGTTTT GAAGGATATG TCCGAGAGAT TAAAATGGTG 301 CAGACTTCTT TGCGGACGAC CGACAACGAA GAAGTCGTGC TGCCCAACAG 351 CGTGGTGATG GGCAACAGCA TCGTCAACCG TTCCACACTG CCGCTGTGCC 401 GCGCCCAAGT GATAGTCGGC GTCGATTACA ACTGCGATTT GAAAGTGGCG 451 AAAGAGGCGG TGTTGAAAGC CGCCGTCGAA CACCCCTTGA GCGTTCAAAA 501 CGAAGAGCGG CAGGCTGCCG CCTACATCAC CGCCTTGGGC GACAATGCCA 551 TCGAAATCAC ATTATGGGCT TGGGCAAACG AAGCAGACCG CTGGACGCTG 601 CAATGCGACT TGAACGAACA AGTGGTCGAA AACCTCCGCA AAGTCAATAT 651 CAACATCCCG TTCCCGCAAC GCGACATACA CATCATCAAT TCTTAA This corresponds to the amino acid sequence <SEQ ID 1764; ORF 579>: m579.pep.. 1 MRAAMTRAQV DATLISFLCN VANIGLLILV IIAALGRLGV STTSVTALIG 51 GAGLAVALSL KDQLSNFAAG ALIILFRPFK VGDFIRVGGF EGYVREIKMV 101 QTSLRTTDNE EWLPNSWM GNSIVNRSTL PLCRAQVIVG VDYNCDLKVA 151 KEAVLKAAVE HPLSVQNEER QAAAYITALG DNAIEITLWA WANEADRWTL 201 QCDLNEQWE NLRKVNINIP FPQRDIHIIN S* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m579/g579 98.7% identity in 231 aa overlap 10 20 30 40 50 60 m579. pep MRAAMTRAQVDATLISFLCΝVAΝIGLLILVIIAALGRLGVSTTSVTALIGGAGLAVALSL II II II II I II II II II II II I I I I I II II II I II II I I II II II II II II II I I I I I M g579 MRAAMTRAQVDATLISFLCΝVAΝIGLLILVIIAALGRLGVSTTSVTALIGGAGLAVALSL 10 20 30 40 50 60 70 80 90 100 110 120 m579.pep KDQLSΝFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDΝEEWLPΝSWM II II II II I II II II II II II I I I I I I I I I I I I I II I I I I II II I I I I I I I I I I I I M M g579 KD Q LSΝFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDΝEEWLPΝSWM 70 80 90 100 110 120 130 140 150 160 170 180 m579. pep GNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQNEERQAAAYITALG II II II I I: II I II I II I II II I II II II II I II II I I: II II II II I II I llllllll g579 GNSIVNRSSLPLCRAQVIVGVDYNCDLKVAKEAVLKAAAEHPLSVQNEERQPAAYITALG 130 140 150 160 170 180 190 200 210 220 230 m579.pep DNAIEITLWAWANEADRWTLQCDLNEQWENLRKVNINIPFPQRDIHIINSX II II II I I II II II I II II I II I II II II II I II II I II I I I I II II II II I g579 DNAIEITLWAWANEADRWTLQCDLNEQWENLRKVNINIPFPQRDIHIINSX 190 200 210 220 230 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1765>: a579. seq 1 ATGAGGGCGG CGATGACGCG CGCGCAGGTC GATGCCACGC TGATTAGTTT 51 TTTGTGTAAT GTTGCCAATA TCGGCTTATT GATTTTGGTG ATTATTGCCG 101 CATTGGGCAG ATTGGGCGTT TCCACAACAT CCGTAACCGC CTTAATCGGC 151 GGCGCGGGTT TGGCGGTGGC GTTGTCCTTG AAAGACCAGC TGTCCAATTT 201 TGCCGCCGGC GCGCTGATTA TCCTGTTCCG CCCGTTCAAA GTCGGCGATT 251 TTATCCGCGT CGGCGGTTTT GAAGGATATG TCCGAGAGAT TAAAATGGTG 301 CAGACTTCTT TGCGGACGAC CGACAACGAA GAAGTCGTGC TGCCCAACAG 351 CGTGGTGATG GGCAACAGCA TCGTCAACCG TTCCACACTG CCGCTGTGCC 401 GCGCCCAAGT GATAGTCGGC GTCGATTACA ACTGCGATTT GAAAGTGGCG 451 AAAGAGGCGG TGTTGAAAGC CGCCGTCGAA CACCCCTTGA GCGTTCAAAA 501 CGAAGAGCGG CAGGCCGCCG CCTACATCAC CGCCTTGGGC GACAATGCCA 551 TCGAAATCAC ATTATGGGCT TGGGCAAACG AAGCAGACCG CTGGACGCTG 601 CAATGCGACT TGAACGAACA AGTGGTCGAA AACCTCCGCA AAGTCAATAT 651 CAACATCCCG TTCCCGCAAC GCGACATACA CATCATCAAT TCTTAA This corresponds to the amino acid sequence <SEQ ID 1766; ORF 579. a>: a579.pep 1 MRAAMTRAQV DATLISFLCN VANIGLLILV IIAALGRLGV STTSVTALIG 51 GAGLAVALSL KDQLSNFAAG ALIILFRPFK VGDFIRVGGF EGYVREIKMV 101 QTSLRTTDNE EWLPNSWM GNSIVNRSTL PLCRAQVIVG VDYNCDLKVA 151 KEAVLKAAVE HPLSVQNEER QAAAYITALG DNAIEITLWA WANEADRWTL 201 QCDLNEQWE NLRKVNINIP FPQRDIHIIN S* m579/a579 100.0% identity in 231 aa overlap 10 20 30 40 50 60 m579. pep MRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGGAGLAVALSL II II II I I II II I II II II II II I I II II I II II II II II II II II II I II II II I I I II a579 MRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGGAGLAVALSL 10 20 30 40 50 60 70 80 90 100 110 120 m579. pep KDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDNEEWLPNSWM II I II I II I II II I II I I II II I II I II II I II II I II II I II I II II I II II II II II I a579 KDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDNEEVVLPNSWM 70 80 90 100 110 120 130 140 150 160 170 180 m579. pep GNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQNEERQAAAYITALG II II II II II I II II I II II II I II II II II II II I I II II II I I II I I I I II I II II I I a579 GNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQNEERQAAAYITALG 130 140 150 160 170 180 190 200 210 220 230 m579.pep DNAIEITLWAWANEADRWTLQCDLNEQWENLRKVNINIPFPQRDIHIINSX I II I I II II II II II II I II II I II I I II II II II I I II I I I I I I II I I II I a579 DNAIEITLWAWANEADRWTLQCDLNEQWENLRKVNINIPFPQRDIHIINSX 190 200 210 220 230 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1767>: g579-l . seq 1 ATGGACTTCA AACAATTTGA TTTTTTACAC CTGATCAGTG TTTCCGGTTG 51 GGGGCATCTG GCTGAAAAGG CGTGGGCGTT CGGGCTGAAC CTTGCCGCCG 101 CGCTGCTTAT TTTCTTGGTC GGGAAATGGG CGGCGAAACG CATTGTCGCC 151 GTAATGAGGG CGGCGATGAC GCGCGCGCAG GTCGATGCCA CGCTGATTAG 201 TTTTTTGTGT AATGTTGCCA ATATCGGCTT ATTGATTTTG GTGATTATTG 251 CCGCATTGGG ACGGTTGGGC GTTTCCACAA CATCCGTAAC CGCCTTAATC 301 GGCGGCGCGG GTTTGGCGGT GGCGTTGTCC TTAAAAGACC AGCTGTCCAA 351 TTTTGCCGCC GGCGCGCTGA TTATCCTGTT CCGCCCGTTC AAAGTCGGCG 401 ACTTTATCCG TGTCGGCGGT TTTGAAGGAT ATGTCCGGGA AATCAAAATG 451 GTGCAGACTT CTTTGCGGAC GACCGACAAC GAAGAAGTCG TGCTGCCCAA 501 CAGCGTGGTG ATGGGCAACA GCATCGTCAA CCGTTCCAGC CTGCCGCTTT 551 GCCGCGCCCA AGTGATAGTC GGCGTCGATT ACAACTGCGA TTTGAAAGTG 601 GCGAAAGAGG CGGTGTTGAA AGCCGCCGCC GAACACCCCT TGAGCGTTCA 651 AAACGAAGAG CGGCAGCCCG CCGCCTACAT CACCGCCTTG GGCGACAATG 701 CCATCGAAAT CACATTATGG GCTTGGGCAA ACGAAGCAGA CCGCTGGACG 751 CTGCAATGCG ACTTGAACGA ACAAGTGGTC GAAAACCTCC GCAAAGTCAA 801 TATCAACATC CCGTTCCCGC AACGCGACAT ACACATCATC AATTCTTAA This corresponds to the amino acid sequence <SEQ ID 1768; ORF 008.ng>: g579-l.pep 1 MDFKQFDFLH LISVSGWGHL AEKAWAFGLN LAAALLIFLV GKWAAKRIVA 51 VMRAAMTRAQ VDATLISFLC NVANIGLLIL VIIAALGRLG VSTTSVTALI 101 GGAGLAVALS LKDQLSNFAA GALIILFRPF KVGDF1RVGG FEGYVREIKM 151 VQTSLRTTDN EEVVLPNSVV MGNSIVNRSΞ LPLCRAQVIV GVDYNCDLKV 201 AKEAVLKAAA EHPLSVQNEE RQPAAYITAL GDNAIEITLW AWANEADRWT 251 LQCDLNEQVV ENLRKVNINI PFPQRDIHII NS* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1769>: m579-l.seq 1 ATGGACTTCA AACAATTTGA TTTTTTACAC CTGATCAGTG TTTCCGGTTG 51 GGAGCATCTG GCTGAAAAGG CGTGGGCGTT CGGGCTGAAC CTTGCCGCCG 101 CGCTGCTTAT TTTTTTGGTC GGAAAATGGG CGGCGAAACG CATTGTCGCT 151 GTGATGAGGG CGGCGATGAC GCGCGCGCAG GTCGATGCCA CGCTGATTAG 201 TTTTTTGTGT AATGTTGCCA ATATCGGCTT ATTGATTTTG GTGATTATTG 251 CCGCATTGGG CAGATTGGGC GTTTCCACAA CATCCGTAAC CGCCTTAATC 301 GGCGGCGCGG GTTTGGCGGT GGCGTTGTCC CTGAAAGACC AGCTGTCCAA 351 TTTTGCCGCC GGCGCACTGA TTATCCTGTT CCGCCCGTTC AAAGTCGGCG 401 ATTTTATCCG CGTCGGCGGT TTTGAAGGAT ATGTCCGAGA GATTAAAATG 451 GTGCAGACTT CTTTGCGGAC GACCGACAAC GAAGAAGTCG TGCTGCCCAA 501 CAGCGTGGTG ATGGGCAACA GCATCGTCAA CCGTTCCACA CTGCCGCTGT 551 GCCGCGCCCA AGTGATAGTC GGCGTCGATT ACAACTGCGA TTTGAAAGTG 601 GCGAAAGAGG CGGTGTTGAA AGCCGCCGTC GAACACCCCT TGAGCGTTCA 651 AAACGAAGAG CGGCAGGCTG CCGCCTACAT CACCGCCTTG GGCGACAATG 701 CCATCGAAAT CACATTATGG GCTTGGGCAA ACGAAGCAGA CCGCTGGACG 751 CTGCAATGCG ACTTGAACGA ACAAGTGGTC GAAAACCTCC GCAAAGTCAA 801 TATCAACATC CCGTTCCCGC AACGCGACAT ACACATCATC AATTCTTAA This corresponds to the amino acid sequence <SEQ ID 1770; ORF 579- 1>: m579-l.pep 1 MDFKQFDFLH LIΞVSGWEHL AEKAWAFGLN LAAALLIFLV GKWAAKRIVA 51 VMRAAMTRAQ VDATLISFLC NVANIGLLIL VIIAALGRLG VSTTSVTALI 101 GGAGLAVALS LKDQLSNFAA GALIILFRPF KVGDFIRVGG FEGYVREIKM 151 VQTSLRTTDN EEVVLPNSVV MGNSIVNRST LPLCRAQVIV GVDYNCDLKV 201 AKEAVLKAAV EHPLSVQNEE RQAAAYITAL GDNAIEITLW AWANEADRWT 251 LQCDLNEQVV ENLRKVNINI PFPQRDIHII NS* m579-l/g579-l 98.6% identity in 282 aa overlap 10 20 30 40 50 60 m579-l .pep MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWAAKRIVAVMRAAMTRAQ llll lllllll I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g579-l MDFKQFDFLHLISVSGWGHLAEKAWAFGLNLAAALLIFLVGKWAAKRIVAVMRAAMTRAQ 10 20 30 40 50 60 70 80 90 100 110 120 m579-1. pep VDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGGAGLAVALSLKDQLSNFAA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g579-l VDATLIΞFLCNVANIGLLILVIIAALGRLGVΞTTSVTALIGGAGLAVALSLKDQLSNFAA 70 80 90 100 110 120 130 140 150 160 170 180 m579-l.pep GALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDNEEVVLPNSVVMGNSIVNRST I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I : g579-l GALIILFRPFKVGDFIRVGGFEGYVREIKMVQTΞLRTTDNEEVVLPNSVVMGNSIVNRSS 130 140 150 160 170 180 190 200 210 220 230 240 m579-l.pep LPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQNEERQAAAYITALGDNAIEITLW I I I I I I I I I I I I I I I I I I I I I I I I I I I I I :l I I I I I I I I I I I l g579-l LPLCRAQVIVGVDYNCDLKVAKEAVLKAAAEHPLSVQNEERQPAAYITALGDNAIEITLW 190 200 210 220 230 240 250 260 270 280 m579-l .pep AWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRDIHIINSX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g579-l AWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRDIHIINSX 250 260 270 280 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1771>: a579-l.seq 1 ATGGACTTCA AACAATTTGA TTTTTTACAC CTGATAAGTG CTTCCGGCTG 51 GGAGCATCTG GCTGAAAAGG CGTGGGCGTT CGGGCTGAAC CTTGCCGCCG 101 CGCTGCTTAT TTTTTTGGTC GGAAAATGGG CGGCGAAACG CATTGTCGCC 151 G GATGAGGG CGGCGATGAC GCGCGCGCAG GTCGATGCCA CGCTGATTAG 201 TTTTTTGTGT AATGTTGCCA ATATCGGCTT ATTGATTTTG GTGATTATTG 251 CCGCATTGGG CAGATTGGGC GTTTCCACAA CATCCGTAAC CGCCTTAATC 301 GGCGGCGCGG GTTTGGCGGT GGCGTTGTCC TTGAAAGACC AGCTGTCCAA 351 TTTTGCCGCC GGCGCGCTGA TTATCCTGTT CCGCCCGTTC AAAGTCGGCG 401 ATTTTATCCG CGTCGGCGGT TTTGAAGGAT ATGTCCGAGA GATTAAAATG 451 GTGCAGACTT CTTTGCGGAC GACCGACAAC GAAGAAGTCG TGCTGCCCAA 501 CAGCGTGGTG ATGGGCAACA GCATCGTCAA CCGTTCCACA CTGCCGCTGT 551 GCCGCGCCCA AGTGATAGTC GGCGTCGATT ACAACTGCGA TTTGAAAGTG 601 GCGAAAGAGG CGGTGTTGAA AGCCGCCGTC GAACACCCCT TGAGCGTTCA 651 AAACGAAGAG CGGCAGGCCG CCGCCTACAT CACCGCCTTG GGCGACAATG 701 CCATCGAAAT CACATTATGG GCTTGGGCAA ACGAAGCAGA CCGCTGGACG 751 CTGCAATGCG ACTTGAACGA ACAAGTGGTC GAAAACCTCC GCAAAGTCAA 801 TATCAACATC CCGTTCCCGC AACGCGACAT ACACATCATC AATTCTTAA This corresponds to the amino acid sequence <SEQ ID 1772; ORF 579-l.a>: a579-l.pep 1 MDFKQFDFLH LISASGWEHL AEKAWAFGLN LAAALLIFLV GKWAAKRIVA 51 VMRAAMTRAQ VDATLISFLC NVANIGLLIL VIIAALGRLG VSTTSVTALI 101 GGAGLAVALS LKDQLSNFAA GALIILFRPF KVGDFIRVGG FEGYVREIKM 151 VQTSLRTTDN EEVVLPNSVV MGNSIVNRST LPLCRAQVIV GVDYNCDLKV 201 AKEAVLKAAV EHPLSVQNEE RQAAAYITAL GDNAIEITLW AWANEADRWT 251 LQCDLNEQVV ENLRKVNINI PFPQRDIHII NS* a579-l/ 579-l 99.6% identity in 282 aa overlap 10 20 30 40 50 60 a579-l.pep MDFKQFDFLHLIΞASGWEHLAEKAWAFGLNLAAALLIFLVGKWAAKRIVAVMRAAMTRAQ I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m579-1 MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWAAKRIVAVMRAAMTRAQ 10 20 30 40 50 60 70 80 90 100 110 120 a579-l.pep VDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGGAGLAVALSLKDQLSNFAA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m579-l VDATLISFLCNVANIGLLILVIIAALGRLGVΞTTSVTALIGGAGLAVALSLKDQLSNFAA 70 80 90 100 110 120 130 140 150 160 170 180 a579-l.pep GALIILFRPFKVGDFIRVGGFEGYVREIKMVQTΞLRTTDNEEVVLPNΞVVMGNSIVNRST I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m579-l GALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDNEEVVLPNSVVMGNSIVNRST 130 140 150 160 170 180 190 200 210 220 230 240 a579-l.pep LPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQNEERQAAAYITALGDNAIEITLW I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m579-l LPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQNEERQAAAYITALGDNAIEITLW 190 200 210 220 230 240 250 260 270 280 a579-l.pep AWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRDIHIINSX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m579-l AWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRDIHIINSX 250 260 270 280 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1773>: g580.seq 1 atggattcgc ccaaggtcgg gtgcgggtgg atggttttgc cgatgtctgc 51 cgcgtcgcag cccatttcga tggcaaggca gacttcgccg atcatgtcgc 101 caccgttcgg accgacaatg ccgccgccga tgatgcggcc ggtttcggca 151 tcgaaaatca gcttggtaaa gccgttgtcg caaccgttgg caatcgcacg 201 accggaagcc gcccatggga agttggcttt ggtaattttg cggcctgatg 251 ctttggcaga caattcggtt tcaccgaccc atgccacttc gggggaagtg 301 tag This corresponds to the amino acid sequence <SEQ ID 1774; ORF 580.ng>: g580.pep.. 1 MDSPKVGCGW MVLPMSAASQ PISMARQTSP IMSPPFGPTM PPPMMRPVSA 51 SKISLVKPLS QPLAIARPEA AHGKLALVIL RPDALADΝSV SPTHATSGEV 101 * The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1775>: m580 -seq.. 1 ATGGATTCGC CCAAGGTCGG GTGCGGGTGG ATGGTTTTGC CGATGTCTGC 51 CGCGTCGCAG CCCATTTCGA TGGCAAGGCA GACTTCGCCG ATCATATCGC 101 CACCGTTCGG ACCGACAATG CCGCCGCCGA TGATGCGGCC GGTTTCGGCA 151 TCAAAAATCA GCTTGGTAAA GCCGTTGTCG CAACCGTTGG CAATCGCACG 201 GCCGGAAGCC GCCCACGGGA AGTTGGCTTT GGTGATTTTG CGGCCGGAGG 251 CTTTGGCGGA CAGTTCGGTT TCGCCCACCC ACGCCACTTC GGGGGAAGTG 301 TAG This corresponds to the amino acid sequence <SEQ ID 1776; ORF 580>: m580.pe .. 1 MDSPKVGCGW MVLPMSAASQ PISMARQTSP IISPPFGPTM PPPMMRPVSA 51 SKISLVKPLS QPLAIARPEA AHGKLALVIL RPEALADSSV SPTHATSGEV 101 * m580/g580 97.0% identity in 100 aa overlap 10 20 30 40 50 60 m580.pep MDSPKVGCGWMVLPMSAASQPISMARQTSPIISPPFGPTMPPPMMRPVSASKISLVKPLS g580 MDS PKVGCGWMVLPMSAASQP I ΞMARQTS PIMS PPFGPTMPPPMMRPVSASKI SLVKPLS 10 20 30 40 50 60 70 80 90 100 πι580 . pep QPLAIARPEAAHGKLALVILRPEALADSSVSPTHATSGEVX g580 QPLAIARPEAAHGKLALVILRPDALADΝSVSPTHATSGEVX 70 80 90 1O0 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1777>: a580. seq 1 ATGGATTCGC CCAAGGTCGG GTGCGGGTGG ATGGTTTTGC CGATGTCTGC 51 CGCGTCGCAG CCCATTTCGA TGGCAAGGCA GACTTCGCCG ATCATGTCGC 101 CACCGTTCGG ACCGACAATG CCGCCGCCGA TGATGCGGCC GGTTTCAGCA 151 TCAAAAATCA GCTTGGTGAA ACCATTGTCG CAACCGTTGG CAATCGCACG 201 GCCGGAAGCA GCCCATGGGA AGTTGGCTTT GGTGATTTTG CGGCCGGAGG 251 CTTTGGCAGA CAATTCGGTT TCGCCCACCC ATGCCACTTC AGGAGAAGTG 301 TAA This corresponds to the amino acid sequence <SEQ ID 1778; ORF 580. a>: a580.pep 1 MDSPKVGCGW MVLPMSAASQ PISMARQTSP IMSPPFGPTM PPPMMRPVSA 51 SKISLVKPLS QPLAIARPEA AHGKLALVIL RPEALADΝSV SPTHATSGEV 101 * m580/a580 98.0% identity in 100 aa overlap 10 20 30 40 50 60 m580.pep MDSPKVGCGWMVLPMSAASQPISMARQTSPIISPPFGPTMPPPMMRPVSASKISLVKPLS II II II II II II II II II I II II II II I II I : II II I II II I I II II II II I II I I II I I a580 MDSPKVGCGWMVLPMSAASQPISMARQTSPIMSPPFGPTMPPPMMRPVSASKISLVKPLS 10 20 30 40 50 60 70 80 90 100 m580. pep QPLAIARPEAAHGKLALVILRPEALADSSVSPTHATSGEVX II II II II II II I II II I II I II I II I : II II II II II I II a580 QPLAIARPEAAHGKLALVILRPEALADNSVSPTHATSGEVX 70 80 90 100 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1779>: g581.seq.. 1 atgcacttcg cccagcttgt gggtcaaacc ggtatagaac aaaatacgtt 51 ctgtcgtcgt ggttttaccc gcatcgatat gggcggaaat accgatgttg 101 cggtacaggc tgatcggggt cttacgagcc attttattag cctttcaaaa 151 ttagaaacgg aagtgagaga atgctttgtt ggcttcagcc atacggtgta 201 cttcttcacg ttttttcaac gcaccgccac ggccttcgga cgcatcaatc 251 aactcgcctg ccaaacgcag atccatggat ttctcaccac gtttgcgggc 301 cgcgtcgcga acccaacgca ttgccaaagc cagacggcgt ga This corresponds to the amino acid sequence <SEQ ID 1780; ORF 581.ng>: g581.pep.. 1 MHFAQLVGQT GIEQΝTFCRR GFTRIDMGGΝ TDVAVQADRG LTSHFISLΞK 51 LETEVRECFV GFSHTVYFFT FFQRTATAFG RIΝQLACQTQ IHGFLTTFAG 101 RVAΝPTHCQS QTA* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1781>: m581.seq.. 1 ATGCACTTCG CCCAGCTTGT GGGTCAAACC GGTATAGAAC AAAATACGTT 51 CTGTCGTCGT GGTTTTACCC GCGTCAATAT GGGCGGAAAT ACCGATGTTA 101 CGGTACAGGC TGATCGGGGT CTTACGAGCC ATTTTATTAG CCTTTCAAAA 151 TTAGAAACGG AAGTGAGAGA ATGCTTTGTT GGCTTCAGCC ATACGGTGTA 201 CTTCTTCACG TTTTTTCAAC GCACCGCCAC GGCCTTCGGA CGCATCAATC 251 AATTCGCCTG CCAAACGCAG GTCCATGGAT TTCTCACCAC GTTTGCGGGC 301 CGCATCGCGA ACCCAGCGCA TTGCCAAAGC CAAACGGCGT GA This corresponds to the amino acid sequence <SEQ ID 1782; ORF 581>: m581.pep.. 1 MHFAQLVGQT GIEQΝTFCRR GFTRVΝMGGΝ TDVTVQADRG LTSHFISLSK 51 LETEVRECFV GFSHTVYFFT FFQRTATAFG RIΝQFACQTQ VHGFLTTFAG 101 RIAΝPAHCQS QTA* m581 / g581 93.8% identity in 113 aa overlap 10 20 30 40 50 60 m581.pep MHFAQLVGQTGIEQΝTFCRRGFTRVΝMGGΝTDVTVQADRGLTSHFISLSKLETEVRECFV g581 MHFAQLVGQTGIEQΝTFCRRGFTRIDMGGΝTDVAVQADRGLTSHFISLSKLETEVRECFV 10 20 30 40 50 60 70 80 90 100 110 m581. pep GFSHTVYFFTFFQRTATAFGRIΝQFACQTQVHGFLTTFAGRIAΝPAHCQSQTAX g581 GFSHTVYFFTFFQRTATAFGRIΝQLACQTQIHGFLTTFAGRVAΝPTHCQSQTAX 70 80 90 100 110 The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 1783>: a581. seq 1 ATGCACTTCG CCCAGCTTGT GGGTCAAACC GGTATAGAAC AAAATACGTT 51 CTGTCGTCGT GGTTTTACCC GCATCGATAT GGGCGGAAAT ACCGATGTTA 101 CGGTACAGGC TGATCGGGGT CTTACGAGCC ATTTTATTAG CCTTTCAAAA 151 TTAGAAACGG AAGTGAGAGA ATGCTTTGTT GGCTTCAGCC AT CGGTGTA 201 CTTCTTCACG TTTTTTCAAC GCACCGCCAC GGCCTTCGGA CGCATCAATC 251 AATTCGCCTG CCAAACGCAG GTCCATGGAT TTCTCACCAC GTTTGCGGGC 301 CGCATCGCGA ACCCAGCGCA TTGCCAAAGC CAAACGGCGT GA This corresponds to the amino acid sequence <SEQ ID 1784; ORF 581.a>: a581.pep 1 MHFAQLVGQT GIEQNTFCRR GFTRIDMGGN TDVTVQADRG LTSHFISLSK 51 LETEVRECFV GFSHTVYFFT FFQRTATAFG RINQFACQTQ VHGFLTTFAG 101 RINPAHCQS QTA* m581/a581 98.2% identity in 113 aa overlap 10 20 30 40 50 60 m581. pep MHFAQLVGQTGIEQNTFCRRGFTRVNMGGNTDVTVQADRGLTSHFISLSKLETEVRECFV II II II II II II II II I II II II I :: II II I II I II II II I I I I II I II II I I II I I I I I a581 MHFAQLVGQTGIEQNTFCRRGFTRIDMGGNTDVTVQADRGLTSHFISLSKLETEVRECFV 10 20 30 40 50 60 70 80 90 100 110 m581.pep GFSHTVYFFTFFQRTATAFGRINQFACQTQVHGFLTTFAGRIANPAHCQSQTAX II II II II II II II II II II II II I I I II I I II I II I I II I II II II I I II I I I a581 GFSHTVYFFTFFQRTATAFGRINQFACQTQVHGFLTTFAGRIANPAHCQSQTAX 70 80 90 100 110 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1785>: g582.seq.. 1 atgcgctata ttcttttgac aggactgttg ccgacggcat ccgcttttgg 51 agagaccgcg ctgcaatgcg ccgctttgac ggacaatgtt acgcgtttgg 101 cgtgttacga caggattttt gcggcacagc ttccgtcttc ggcagggcag 151 gaagggcagg agtcgaaagc cgtactcaat ctgacggaaa ccgtccgcag 201 cagcttggat aagggcgagg cggtcattgt tgttgaaaaa ggcggggatg 251 cgcttcctgc cgacagtgcg ggcgaaaccg ccgatatcta tacgcctttg 301 agcctgatgt acgacttgga caaaaacgat ttgcgcgggc tgttgggcgt 351 acgcgaacac aatccgatgt accttatgcc gttttggtat aacaattcgc 401 ccaactatgc cccgagttcg ccgacgcgcg gtacgactgt acaggaaaaa 451 ttcggacagc agaaacgtgc ggaaaccaaa ttgcaggttt cgttcaaaag 501 caaaattgcc gaaaatttgt ttaaaacccg cgcggatctg tggttcggct 551 acacccaaag atccgattgg cagatttaca accaaggcag gaaatccgcg 601 ccgttccgca atacggatta caaacctgaa attttcctga cccagcctgt 651 gaaggcggat ttgccgttcg gcggcaggct gcgtatgctc ggtgcgggtt 701 ttgtccacca gtccaacgga cagagccgtc ccgaatcgcg ttcgtggaac 751 aggatttatg ccatggcagg catggaatgg ggcaaattga cggtgattcc 801 gcgcgtgtgg gtgcgtgcgt tcgatcagag cggcgataaa aacgacaatc 851 ccgatattgc cgactatatg gggtatggcg acgtgaagct gcagtaccgc 901 ctgaacgaca ggcagaatgt gtattccgta ttgcgctaca accccaaaac 951 gggctacggc gcgattgaag ccgcctacac gtttccgatt aagggcaaac 1001 tcaaaggcgt ggtacgcgga ttccacggtt acggcgagag cctgatcgac 1051 tacaaccaca agcagaacgg tatcggtatc gggttgatgt tcaacgactg 1101 ggacggcatc tga This corresponds to the amino acid sequence <SEQ ID 1786; ORF 582.ng>: g582.pep .. 1 MRYILLTGLL PTASAFGETA LQCAALTDΝV TRLACYDRIF AAQLPSSAGQ 51 EGQESKAVLΝ LTETVRSSLD KGEAVIWEK GGDALPADΞA GETADIYTPL 101 SLMYDLDKΝD LRGLLGVREH ΝPMYLMPFWY ΝΝSPΝYAPSS PTRGTTVQEK 151 FGQQKRAETK LQVSFKSKIA EΝLFKTRADL WFGYTQRSDW QIYΝQGRKSA 201 PFRΝTDYKPE IFLTQPVKAD LPFGGRLRML GAGFVHQSΝG QSRPESRSWΝ 251 RIYAMAGMEW GKLTVIPRVW VRAFDQSGDK ΝDΝPDIADYM GYGDVKLQYR 301 LΝDRQΝVYSV LRYΝPKTGYG AIEAAYTFPI KGKLKGWRG FHGYGESLID 351 YΝHKQΝGIGI GLMFΝDWDGI * The following partial DNA sequence was identified in N meningitidis <SEQ ID 1787>: m582.seq 1 ATGCGCTATA TTCTTTTGAC AGGACTGTTG CCGATGGCAT CCGCTTTTGG 51 AGAGACCGCG CTGCAATGCG CCGCTTTGAC GGACAATGTT ACGCGTTTGG 101 CGTGTTACGA CAGGATTTTT GCGGCACAGC TTCCGTCTTC GGCAGGGCAG 151 GAAGGGCAGG AGTCGAAAGC CGTACTCAAT CTGACGGAAA CCGTCCGCAG 201 CAGCCTGGAT AAGGGCGAGG CGGTCATTGT TGTTGAAAAA GGCGGGGATG 251 CGCTTCCTGC CGACAGTGCG GGCGAAACCG CCGACATCTA TACGCCTTTG 301 AGCCTGATGT ACGACTTGGA CAAAAACGAT TTGCGCGGGC TGTTGGGCGT 351 ACGCGAACAC AATCCGATGT ACCTTATGCC GCTCTGGTAC AACAATTCGC 401 CCAACTATGC CCCGGGTTCG CCGACGCGCG GTACGACTGT ACAGGAAAAA 451 TTCGGACAGC AGAAACGTGC GGAAACCAAA TTGCAGGTTT CGTTCAAAAG 501 CAAAATTGCC GAAGATTTGT TTAAAACCCG CGCGGATCTG TGGTTCGGCT 551 ACACCCAAAG ATCCGATTGG CAGATTTACA ACCAAGGCAG GAAATCCGCG 601 CCGTTCCGCA ATACGGATTA CAAACCTGAA ATTTTCCTGA CCCAGCCTGT 651 GAAGGCGGAT TTGCCGTTCG GCGGCAGGCT GCGTATGCTC GGTGCGGGTT 701 TTGTCCACCA GTCCAACGGA CAGAGCCGTC CCGAATCGCG TTCGTGGAAC 751 AGGATTTACG CCATGGCAGG CATGGAATGG GGCAAATTGA CGGTGATTCC 801 GCGCGTGTGG GTGCGTGCGT TCGATCAGAG CGGCGATAAA AACGACAATC 851 CCGATATTGC CGACTATATG GGGTATGGCG ACGTGAAGCT GCAGTACCGC 901 CTGAACGACA GGCAGAATGT GTATTCCGTA TTGCGCTACA ACCCCAAAAC 951 GGGCTACGGC GCGATTGAAG CCGCCTACAC GTTTCCGATT AAGGGCAAAC 1001 TCAAAGGCGT GGTACGCGGA TTCCACGGTT ACGGCGAGAG CCTGATCGAC 1051 TACAACCACA AGCAGAACGG TATCGGTATC GGGTTGATGT TCAACGACTT 1101 GGACGGCATC TGA This corresponds to the amino acid sequence <SEQ ID 1788; ORF 582>: m582.pep 1 MRYILLTGLL PMASAFGETA LQCAALTDΝV TRLACYDRIF AAQLPSSAGQ 51 EGQESKAVLΝ LTETVRSSLD KGEAVIWEK GGDALPADSA GETADIYTPL 101 SLMYDLDKΝD LRGLLGVREH ΝPMYLMPLWY ΝΝSPΝYAPGS PTRGTTVQEK 151 FGQQKRAETK LQVSFKSKIA EDLFKTRADL WFGYTQRSDW QIYΝQGRKSA 201 PFRΝTDYKPE IFLTQPVKAD LPFGGRLRML GAGFVHQSΝG QSRPESRSWΝ 251 RIYAMAGMEW GKLTVIPRVW VRAFDQSGDK ΝDΝPDIADYM GYGDVKLQYR 301 LΝDRQΝVYSV LRYΝPKTGYG AIEAAYTFPI KGKLKGWRG FHGYGESLID 351 YΝHKQΝGIGI GLMFΝDLDGI * m582 / g582 98.6% identity in 370 aa overlap 10 20 30 40 50 60 m582.pep MRYILLTGLLPMASAFGETALQCAALTDΝVTRLACYDRIFAAQLPSSAGQEGQESKAVLΝ g582 MRYILLTGLLPTASAFGETALQCAALTDΝVTRLACYDRIFAAQLPSSAGQEGQESKAVLΝ 10 20 30 40 50 60 70 80 90 100 110 120 m582 . pep LTETVRSSLDKGEAVIWEKGGDALPADSAGETADIYTPLΞLMYDLDKΝDLRGLLGVREH I I I I I I I I I I I I I I I I I I I I I I I I I M I I I I I I I M I I I I I I I M I M M M M I I I I I I g582 LTETVRSSLDKGEAVIWEKGGDALPADSAGETADIYTPLSLMYDLDKΝDLRGLLGVREH 70 80 90 100 110 120 130 140 150 160 170 180 m582 . pep ΝPMYLMPLWYΝΝSPΝYAPGSPTRGTTVQEKFGQQKRAETKLQVSFKSKIAEDLFKTRADL M I ! I I I M MM IM M I M : IM I MMM g582 ΝPMYLMPFWYΝΝSPΝYAPSSPTRGTTVQEKFGQQKRAETKLQVSFKSKIAEΝLFKTRADL 130 140 150 160 170 180 190 200 210 220 230 240 TT1582.pep WFGYTQRSDWQIYΝQGRKSAPFRΝTDYKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSΝG g582 WFGYTQRSDWQIYΝQGRKSAPFRΝTDYKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSΝG 190 200 210 220 230 240 250 260 270 280 290 300 m582.pep QSRPESRSWΝRIYAMAGMEWGKLTVIPRVWVRAFDQSGDKΝDΝPDIADYMGYGDVKLQYR g582 QSRPESRSWΝRIYAMAGMEWGKLTVIPRVWVRAFDQSGDKΝDΝPDIADYMGYGDVKLQYR 250 260 270 280 290 300 310 320 330 340 350 360 m582.pep LNDRQNVYSVLRYNPKTGYGAIEAAYTFPIKGKLKGWRGFHGYGESLIDYNHKQNGIGI g582 LNDRQNVYSVLRYNPKTGYGAIEAAYTFPIKGKLKGWRGFHGYGESLIDYNHKQNGIGI 310 320 330 340 350 360 370 m582. pep GLMFNDLDGIX llll g582 GLMFNDWDGIX 370 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1789>: a582. seq 1 ATGCGCTATA TTCTTTTGAC AGGACTGTTG CCGATGGCAT CCGCTTTTGG 51 AGAGACCGCG CTGCAATGCG CCGCTTTGAC GGACAATGTT ACGCGTTTGG 101 CGTGTTACGA CAGGATTTTT GCGGCACAGC TTCCGTCTTC GGCAGGGCAG 151 GAAGGGCAGG AGTCGAAAGC CGTACTCAAT CTGACGGAAA CCGTCCGCAG 201 CAGCCTGGAT AAGGGCGAGG CGGTCATTGT TGTTGAAAAA GGCGGGGATG 251 CGCTTCCTGC CGACAGTGCG GGCGAAACCG CCGACATCTA TACGCCTTTG 301 AGCCTGATGT ACGACTTGGA CAAAAACGAT TTGCGCGGGC TGTTGGGCGT 351 ACGCGAACAC AATCCGATGT ACCTTATGCC GCTCTGGTAC AACAATTCGC 401 CCAACTATGC CCCGGGTTCG CCGACGCGCG GTACGACTGT ACAGGAAAAA 451 TTCGGACAGC AGAAACGTGC GGAAACCAAA TTGCAGGTTT CGTTCAAAAG 501 CAAAATTGCC GAAGATTTGT TTAAAACCCG CGCGGATCTG TGGTTCGGCT 551 ACACCCAAAG ATCCGATTGG CAGATTTACA ACCAAGGCAG GAAATCCGCG 601 CCGTTCCGCA ATACGGATTA CAAACCTGAA ATTTTCCTGA CCCAGCCTGT 651 GAAGGCGGAT TTGCCGTTCG GCGGCAGGCT GCGTATGCTC GGTGCGGGTT 701 TTGTCCACCA GTCCAACGGA CAGAGCCGTC CCGAATCGCG TTCGTGGAAC 751 AGGATTTACG CCATGGCAGG CATGGAATGG GGCAAATTGA CGGTGATTCC 801 GCGCGTGTGG GTGCGTGCGT TCGATCAGAG CGGCGATAAA AACGACAATC 851 CCGATATTGC CGACTATATG GGGTATGGCG ACGTGAAGCT GCAGTACCGC 901 CTGAACGACA GGCAGAATGT GTATTCCGTA TTGCGCTACA ATCCCAAAAC 951 GGGCTACGGC GCGATTGAAG CCGCCTACAC GTTTCCGATT AAGGGCAAAC 1001 TCAAAGGCGT GGTACGCGGA TTCCACGGTT ACGGCGAGAG CCTGATCGAC 1051 TACAACCACA AGCAGAACGG TATCGGTATC GGGTTGATGT TCAACGACTT 1101 GGACGGCATC TGA This corresponds to the amino acid sequence <SEQ ID 1790; ORF 582.a>: a582.pep 1 MRYILLTGLL PMASAFGETA LQCAALTDΝV TRLACYDRIF AAQLPSSAGQ 51 EGQESKAVLΝ LTETVRSSLD KGEAVIWEK GGDALPADSA GETADIYTPL 101 SLMYDLDKΝD LRGLLGVREH ΝPMYLMPLWY ΝΝSPΝYAPGS PTRGTTVQEK 151 FGQQKRAETK LQVSFKSKIA EDLFKTRADL WFGYTQRSDW QIYΝQGRKSA 201 PFRΝTDYKPE IFLTQPVKAD LPFGGRLRML GAGFVHQSΝG QSRPESRSWΝ 251 RIYAMAGMEW GKLTVIPRVW VRAFDQSGDK ΝDΝPDIADYM GYGDVKLQYR 301 LΝDRQΝVYSV LRYΝPKTGYG AIEAAYTFPI KGKLKGWRG FHGYGESLID 351 YΝHKQΝGIGI GLMFΝDLDGI * m582/a582 100.0% identity in 370 aa overlap 10 20 30 40 50 60 m582.pep MRYILLTGLLPMASAFGETALQCAALTDΝVTRLACYDRIFAAQLPSSAGQEGQESKAVLΝ II II I I II II II II II II II I II I II I II II I I II I I II II II I I I II I II I II I I I I I I a582 MRYILLTGLLPMASAFGETALQCAALTDΝVTRLACYDRIFAAQLPSSAGQEGQESKAVLΝ 10 20 30 40 50 60 70 80 90 100 110 120 m582.pep LTETVRSSLDKGEAVIWEKGGDALPADSAGETADIYTPLSLMYDLDKΝDLRGLLGVREH I II I II I II I II II II I II II I I I I I II I II I I I I I I I M M I M I M I I I I M I M M I a582 LTETVRSSLDKGEAVIWEKGGDALPADSAGETADIYTPLSLMYDLDKΝDLRGLLGVREH 70 80 90 100 110 120 130 140 150 160 170 180 m582. pep NPMYLMPLWYNNSPNYAPGSPTRGTTVQEKFGQQKRAETKLQVSFKSKIAEDLFKTRADL I II I II II II II II I II II II II II II II II II I I I I II II II I II I I I II II II I I I I I a582 NPMYLMPLWYNNSPNYAPGSPTRGTTVQEKFGQQKRAETKLQVSFKSKIAEDLFKTRADL 130 140 150 160 170 180 190 200 210 220 230 240 m582.pep WFGYTQRSDWQIYNQGRKSAPFRNTDYKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNG II II II II II I II I II II II I I II II II II II II II II I I I I II II I I II I I I I I I II II a582 WFGYTQRSDWQIYNQGRKSAPFRNTDYKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNG 190 200 210 220 230 240 250 260 270 280 290 300 m582. pep QSRPESRSWNRIYAMAGMEWGKLTVIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYR I II II II II II II II II II I I II II II I II II II II I I II I I II II I I II I I I I I I I I I I a582 QSRPESRSWNRIYAMAGMEWGKLTVIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYR 250 260 270 280 290 300 310 320 330 340 350 360 m582.pep LNDRQNVYSVLRYNPKTGYGAIEAAYTFPIKGKLKGWRGFHGYGESLIDYNHKQNGIGI II II II II II II II I II II II II II II II II I I II II I II I I I II II II II II M I I M I a582 LNDRQNVYSVLRYNPKTGYGAIEAAYTFPIKGKLKGWRGFHGYGESLIDYNHKQNGIGI 310 320 330 340 350 360 370 m582.pep GLMFNDLDGIX I II II II II II a582 GLMFNDLDGIX 370 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1791>: g583.seq.. 1 atgataattg accaaagcca aatatttacc catcttgcct tctgtgcctt 51 ttgcgggatt ggagccgtaa ctgccggcaa tcgactgcat aatcggatgt 101 ataatgccgc cgccgcgcgc ggtattggaa ggggtaacgg gagccagcag 151 cagttcggaa agagcgagac tgtaaccgat gcccagcgtt tttcttccaa 201 aaacggcgat aaacaaatat ccgatacgca tccccagccc tgttttgagc 251 aaaccgcgcg aaatcataac tgcgatggca atcagccaaa tcaacggatt 301 ggcgaacgca ctcaacgcat cgctcatcgc cgcgcccggt ttgtcggcgg 351 ttacgccggt tactgcgacc aacccgacgg caataatcga cagcgcgccc 401 aacggcataa ccttgccgat aatggcggca atcacaccga caaacatagc 451 cagcagcgtc caagcctgag gcttgacccc gtcgggtacg ggcagtgcca 501 aaaccagggc gcacaatact gcggcaatgg cgaggggtat cggtttgaaa 551 cccaatttca tcatattgac ctccgtaaaa aagaccgtcc cgaaaaatcg 601 gaaaaataa This corresponds to the amino acid sequence <SEQ ID 1792; ORF 583.ng>: g583.pep.. 1 MIIDQSQIFT HLAFCAFCGI GAVTAGΝRLH ΝRMYΝAAAAR GIGRGΝGSQQ 51 QFGKSETVTD AQRFSSKΝGD KQISDTHPQP CFEQTARΝHΝ CDGΝQPΝQRI 101 GERTQRIAHR RARFVGGYAG YCDQPDGΝΝR QRAQRHΝLAD ΝGGΝHTDKHS 151 QQRPSLRLDP VGYGQCQΝQG AQYCGΝGEGY RFETQFHHID LRKKDRPEKS 201 EK* The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 1793>: m583.seq.. 1 ATGATAGTTG ACCAAAGCCA AATATTTACC CATCTTGCCT TCTGTGCCTT 51 TTGCGGGATT GGAGCCGTAA CTGCCGGCAA TCGACTGCAT AATCGGATGT 101 ATAATGCCGC CGCCGCGCGC GGTATTGGAA GGGGTAACGG GAGCCAGCAG 151 CAGTTCGGAA AGAGCGAGAC TGTAACCGAT GCCCAGCGTT TTTCTTCCAA 201 AAACGGCGAT AAACAAATAT CCGATACGCA TCCCCAGCCC TGTTTTGAGC 251 AAACCGCGCG AAATCATAAC TGCGATGGCA ATCAGCCAAA TCAACGGATT 301 GGCGAACGCA CTCAACGCAT CGCTCATCGC CGCGCCCGGT TTGTCGGCGG 351 TTACGCCGGT TACTGCGACC AACCCGACGG CAATAATCGA CAGCGCGCCC 401 AACGGCATGG CCTTGCCGAT AATGGCGGCA ATCACACCGA CAAACATGGC 451 CAGCAGCGTC CAAGCCTGAG GCTTGACCCC GTCGGGTACG GGCAGTGCCA 501 AAACCAGGGC GCACAATACT GCGGCAATGG CGAGGGGTAT CGGTTTGAAA 551 CCCAATTTCA TCATATTGAC CTCCGTAAAA AAGACCGTCC CGAAAAATCG 601 GAAAAATAA This corresponds to the amino acid sequence <SEQ ID 1794; ORF 583>: m583. e .. 1 MIVDQSQIFT HLAFCAFCGI GAVTAGNRLH NRMYNAAAAR GIGRGNGSQQ 51 QFGKSETVTD AQRFSSKNGD KQISDTHPQP CFEQTARNHN CDGNQPNQRI 101 GERTQRIAHR RARFVGGYAG YCDQPDGNNR QRAQRHGLAD NGGNHTDKHG 151 QQRPSLRLDP VGYGQCQNQG AQYCGNGEGY RFETQFHHID LRKKDRPEKS 201 EK* m583 / g583 98.5% identity in 202 aa overlap 10 20 30 40 50 60 m583.pep MIVDQSQIFTHLAFCAFCGIGAVTAGNRLHNRMYNAAAARGIGRGNGSQQQFGKSETVTD g583 MIIDQSQIFTHLAFCAFCGIGAVTAGNRLHNRMYNAAAARGIGRGNGSQQQFGKSETVTD 10 20 30 40 50 60 70 80 90 100 110 120 m583.pep AQRFSSKNGDKQIΞDTHPQPCFEQTARNHNCDGNQPNQRIGERTQRIAHRRARFVGGYAG I I I I I M M I I M I I M I I M I I I I I I I I I I I I I I I I I I M I I I I I I I I I M I M I I I M g583 AQRFSSKNGDKQISDTHPQPCFEQTARNHNCDGNQPNQRIGERTQRIAHRRARFVGGYAG 70 80 90 100 110 120 130 140 150 160 170 180 m583. pep YCDQPDGNNRQRAQRHGLADNGGNHTDKHGQQRPSLRLDPVGYGQCQNQGAQYCGNGEGY g583 YCDQPDGNNRQRAQRHNLADNGGNHTDKHSQQRPSLRLDPVGYGQCQNQGAQYCGNGEGY 130 140 150 160 170 180 190 200 m583. pep RFETQFHHIDLRKKDRPEKSEKX g583 RFETQFHHIDLRKKDRPEKSEKX 190 200 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1795>: a583. seq 1 ATGATAGTTG ACCAAAGCCA AATATTTACC CATCTTGCCT TCTGTGCCTT 51 TTGCGGGATT GGAGCCGTAA CTGCCGGCAA TCGACTGCAT AATCGGATGT 101 ATAATGCCGC CGCCGCGCGC GGTATTGGAA GGGGTAACGG GAGCCAGCAG 151 CAGTTCGGAA AGAGCGAGAC TGTAACCGAT GCCCAGCGTT TTTCTTCCAA 201 AAACGGCGAT AAACAAATAT CCGATACGCA TCCCCAGCCC TGTTTTGAGC 251 AAACCGCGCG AAATCATAAC TGCGATGGCA ATCAGCCAAA TCAACGGATT 301 GGCGAACGCA CTCAACGCAT CGCTCATCGC CGCACCCGGT TTGTCGGCGG 351 TTACGCCGGT TACTGCGACC AACCCGACGG CAATAATCGA CAGCGCACCC 401 AACGGCATGG CCTTGCCGAT AATGGCGGCA ATCACACCGA TAAACATGGC 451 CAGCAGCGTC CAAGCCTGAG GCTTGACCCC GTCGGGTACG GGCAGTGCCA 501 AAACCAAGGC GCACAATACT GCGGCAATGG CGAGGGGTAT CGGTTTGAAA 551 CCCAATTTCA TCATATTGAC CTCCGTAAAA AAGACCGTCC CGAAAAATCG 601 GAAAAATAA This corresponds to the amino acid sequence <SEQ ID 1796; ORF 583. a>: a583.pep 1 MIVDQSQIFT HLAFCAFCGI GAVTAGΝRLH ΝRMYΝAAAAR GIGRGΝGSQQ 51 QFGKSETVTD AQRFSSKΝGD KQISDTHPQP CFEQTARΝHΝ CDGΝQPΝQRI 101 GERTQRIAHR RTRFVGGYAG YCDQPDGΝΝR QRTQRHGLAD ΝGGΝHTDKHG 151 QQRPSLRLDP VGYGQCQΝQG AQYCGΝGEGY RFETQFHHID LRKKDRPEKS 201 EK* m583/a583 99.0% identity in 202 aa overlap 10 20 30 40 50 60 m583.pep MIVD Q SQIFTHLAFCAFCGIGAVTAGΝRLHΝRMYΝAAAARGIGRGΝGSQQQFGKSETVTD M I I M I I M II II I I I I I II I I II II II I I I I M I I I I I M M II I I I I II I I II II I I a583 MIVDQSQIFTHLAFCAFCGIGAVTAGNRLHNRMYNAAAARGIGRGNGSQQQFGKSETVTD 10 20 30 40 50 60 70 80 90 100 110 120 m583.pep AQRFSSKNGDKQISDTHPQPCFEQTARNHNCDGNQPNQRIGERTQRIAHRRARFVGGYAG II I II I II II II II II I I II II II I I II II I II I I II II II II II II II II : I II I II II a583 AQRFSSKNGDKQISDTHPQPCFEQTARNHNCDGNQPNQRIGERTQRIAHRRTRFVGGYAG 70 80 90 100 110 120 130 140 150 160 170 180 m583. pep YCDQPDGNNRQRAQRHGLADNGGNHTDKHGQQRPSLRLDPVGYGQCQNQGAQYCGNGEGY II II II II I II I : II I II I II II II I I I II II I I I II II II II II II II II II II I I II I a583 YCDQPDGNNRQRTQRHGLADNGGNHTDKHGQQRPSLRLDPVGYGQCQNQGAQYCGNGEGY 130 140 150 160 170 180 190 200 m583. pep RFETQFHHIDLRKKDRPEKSEKX II II II II II II II II I I II II I a583 RFETQFHHIDLRKKDRPEKSEKX 190 200 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1797>: g584.seq.. 1 atgctgcgtt ctattttggc ggcttccctg ctggcggtat cttttccggc 51 ggcggctgag gcattgaatt acaatattgt cgaattttcc gaatcggcgg 101 gtatcgaggt ggctcaggat acaatgtccg cgcgtttcca ggtggcggcg 151 gaaggacggg acaaaaatgc cgtcaatgcc gagtttgtta aaaaattcaa 201 caatttcacc agaaaatcga aaaatggtag ctttaaaacc gaattggtat 251 cgcgcagtgc gatgccgcgc tatcaatata ccaacggcag acgcattcaa 301 acaggctggg aggagcgtgc ggaatttaag gcggagggca gggattttga 351 tgctttaaac cgttttattg ctgatgttca gacggatgct tcgcttgaag 401 ataccgattt cagcgtgtcg cgcgaacgcc gaaacgaggt catcgatcag 451 gtcagcaagg atgccgtttt gcgtttcaag gcgcgtgccg aaaaactggc 501 gggcgttctg ggtgcgtccg gttataaaat cgtcaaattg aattttgggc 551 aaatcggcag ccatattgcg ggcgatgggg ctgttcgggc aaaaatgctg 601 cgcgcgatgc cgatggcggc aagcgtcaat atgaagggta cggattcagc 651 cgcaccgggt gtggaggaaa tcagcatcag catcaatggg acggttcagt 701 tctaa This corresponds to the amino acid sequence <SEQ ID 1798; ORF 584.ng>: g584.pep Length: .. 1 MLRSILAASL LAVSFPAAAE ALΝYΝIVEFS ESAGIEVAQD TMSARFQVAA 51 EGRDKΝAVΝA EFVKKFΝΝFT RKSKΝGSFKT ELVSRSAMPR YQYTΝGRRIQ 101 TGWEERAEFK AEGRDFDALΝ RFIADVQTDA SLEDTDFSVS RERRΝEVIDQ 151 VSKDAVLRFK ARAEKLAGVL GASGYKIVKL ΝFGQIGSHIA GDGAVRAKML 201 RAMPMAASVΝ MKGTDSAAPG VEEISISIΝG TVQF* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1799>: m584.seq.. 1 ATGTTGCGTC TTGTTTTGGC GGCTTCGCTG TCGGCGGTAT CTTTTCCGGC 51 AGCGGCTGAA GCATTGAATT ACAATATTGT CGAATTTTCC GAATCGGCGG 101 GTGTCGAGGT GGCTCAGGAT ACAATGTCCG CACGTTTCCA AGTGACGGCG 151 GAAGGACGGG ACAAAAATGC CGTCAATGCT GAGTTTGTTA AAAAATTCAA 201 CAAGTTCATC AGAAAATCGA AAAATGGTAG CTTTAAAACC GAATTGGTAT 251 CGCGCAGTGC GATGCCGCGC TATCAATATA CCAACGGCAG ACGCATTCAA 301 ACAGGCTGGG AGGAGCGTGC GGAATTTAAG GTCGAAGGTA GAGATTTTGA 351 TGAGTTAAAC CGTTTTATTG CCGATATTCA AGCAGATGCC GCGTTGGmAT 401 ATACGGATTT CCATGTGTCG CGCGAACGCC GCAACGAGGT CATC ATCAG 451 GTCAGCAAGG ATGCCGTTTT GCGTTTCAAG GCGCGTGCCG AAAAGTTGGC 501 GGGCGTTTTG GGTGCGTCCG GTTATAAAAT CGTCAAATTG AATTTGGGAC 551 ACATCGGCAG CCATATCGCG GGAGGGGGAG CTGCTCAGGC AAAAATGCTT 601 CGTGCCATGC CGATGGCGGC AAGCGTCAAT ATGGAGGGTG CGGATTCCGC 651 CGCGCCTGGT GTGGAGGAAA TCAGCATCAG CGTCAATGGG ACGGTTCAGT 701 TCTGA This corresponds to the amino acid sequence <SEQ ID 1800; ORF 584>: m58 .pep.. 1 MLRLVLAAΞL SAVSFPAAAE ALNYNIVEFS ESAGVEVAQD TMSARFQVTA 51 EGRDKNAVNA EFVKKFNKFI RKSKNGSFKT ELVSRSAMPR YQYTNGRRIQ 101 TGWEERAEFK VEGRDFDELN RFIADIQADA ALXYTDFHVS RERRNEVIXQ 151 VSKDAVLRFK ARAEKLAGVL GASGYKIVKL NLGHIGΞHIA GGGAAQAKML 201 RAMPMAASVN MEGADSAAPG VEEIΞISVNG TVQF* m584 / g584 89.7% identity in 234 aa overlap 10 20 30 40 50 60 m584. pep MLRLVLAASLSAVSFPAAAEALNYNIVEFSESAGVEVAQDTMSARFQVTAEGRDKNAVNA III MINI g584 MLRSILAASLLAVSFPAAAEALNYNIVEFSESAGIEVAQDTMSARFQVAAEGRDKNAVNA 10 20 30 40 50 60 70 80 90 100 110 120 m584.pep EFVKKFNKFIRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTGWEERAEFKVEGRDFDELN MINIMI II g584 EFVKKFNNFTRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTGWEERAEFKAEGRDFDALN 70 80 90 100 110 120 130 140 150 160 170 180 m584.pep RFIADIQADAALXYTDFHVΞRERRNEVIXQVSKDAVLRFKARAEKLAGVLGAΞGYKIVKL I M I h M I M I I I I l l l l l l l l l l 1 1 1 1 1 1 1 1 1 I I 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 g584 RFIADVQTDASLEDTDFΞVSRERRNEVIDQVSKDAVLRFKARAEKLAGVLGASGYKIVKL 130 140 150 160 170 180 190 200 210 220 230 m584. pep NLGHIGSHIAGGGAAQAKMLRAMPMAASVNMEGADSAAPGVEEISISVNGTVQFX h h l l l l l l l : - I I ; M M I I M M M I I I M I M M M I M g584 NFGQIGSHIAGDGAVRAKMLRAMPMAASVNMKGTDSAAPGVEEISIΞINGTVQFX 190 200 210 220 230 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1801>: a584. seq 1 ATGTTGCGTT CTATTTTGGC GGCTTCCCTG CTG 51 ATTGT CGAATTTTCT GAATCGGCGG 101 GTGTCGAGGC GGTTCAGGAT ACAATGTCCG CACGTTTCCA AGTGACGGCG 151 GAAGGACGGG ACAAAAATGC CGTCAATGCC GAGTTTGTTA AAAAATTCAA 201 CAATTTCACC AGAAAATCAA AAAATGGTAG CTTTAAAACC GAATTGGTAT 251 CGCGCAGTGC GATGCCGCGC TATCAATATA CCAACGGCAG ACGCATTCAA 301 ACAGGTTGGG AGGAGCGTGC GGAATTTAAG GTCGAGGGTA GGAATTTTGA 351 TGCGTTGAAC CGTTTTATTG CCGATGTTCA GGCAGATGCC GCGTTGGAAT 401 ATACGGATTT CCATGTGTCG CGCGAACGCC GCAACGAGGT CATCGATCAG 451 GTCAGCAAGG ATGCCGTTTT GCGTTTCAAG GCGCGTGCCG AAAAGTTGGC 501 GGGCGTTTTG GGTGCGTCCG GTTATAAAAT CGTCAAATTG AATTTGGGAC 551 ACATCGGCAG CCATATCGCG GGAGGGGGAG CTGCTCAGGC AAAAATGCTT 601 CGTGCCATGC CGATGGCGGC AAGCGTCAAT ATGGAGGGTG CGGATTCCGC 651 CGCGCCTGGT GTGGAGGAAA TCAGCATCAG CGTCAATGGG ACGGTTCAGT 701 TCTGA This corresponds to the amino acid sequence <SEQ ID 1802; ORF 584.a>: a584.pep 1 MLRSILAASL L IVEFS ESAGVEAVQD TMSARFQVTA 51 EGRDKΝAVΝA EFVKKFΝΝFT RKSKΝGSFKT ELVSRSAMPR YQYTΝGRRIQ 101 TGWEERAEFK VEGRΝFDALΝ RFIADVQADA ALEYTDFHVS RERRΝEVIDQ 151 VSKDAVLRFK ARAEKLAGVL GASGYKIVKL ΝLGHIGSHIA GGGAAQAKML 201 RAMPMAASVΝ MEGADSAAPG VEEISISVΝG TVQF* m584/a584 88.9% identity in 234 aa overlap 10 20 30 40 50 60 m584 •pep MLRLVLAASLSAVSFPAAAEALΝYΝIVEFSESAGVEVAQDTMSARFQVTAEGRDKΝAVΝA Ml MMM I M I M M I IM: I I I II I M I I I I I I I I I I I I I I a584 MLRSILAASLL IVEFSESAGVEAVQDTMSARFQVTAEGRDKΝAVΝA 10 20 30 40 70 80 90 100 110 120 m584. pep EFVKKFNKFIRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTGWEERAEFKVEGRDFDELN II I II II : I I II I II I II II I II II II II I II II II II II II II II II II I I I : II II a584 EFVKKFNNFTRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTGWEERAEFKVEGRNFDALN 50 60 70 80 90 100 130 140 150 160 170 180 m584.pep RFIADIQADAALXYTDFHVΞRERRNEVIXQVSKDAVLRFKARAEKLAGVLGASGYKIVKL II II I : II I II I lllllllllllllll I II II II II I II II II II II I I II II II I I I a58 RFIADVQADAALEYTDFHVSRERRNEVIDQVSKDAVLRFKARAEKLAGVLGASGYKIVKL 110 120 130 140 150 160 190 200 210 220 230 m584.pep NLGHIGSHIAGGGAAQAKMLRAMPMAASVNMEGADSAAPGVEEISISVNGTVQFX II I II II II II I I I II I II II I II I II II II I II II II II II II II II II I I I I I a584 NLGHIGSHIAGGGAAQAKMLRAMPMAASVNMEGADSAAPGVEEISISVNGTVQFX 170 180 190 200 210 220 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1803>: g585.seq.. 1 atgaaactgt tccaacgcat tttcgccaca ttttgcgcgg ttatcgtctg 51 cgcaatcttt gtggcgagtt tttctttttg gctggtgcag aacacccttg 101 ccgaaaacca attcaaccaa cgccgcacca tcgaaaccac attgatgggc 151 agcattattt ccgcattcaa gacacggggc gacaacggcg cgcgcgaaat 201 cctgaccgaa tggaaaaaca gccccgtctc atccgccgtt tacgtcatac 251 agggcgacga gaaaaaagac atcttaaacc gctatatcga caattacacc 301 atagaacgcg cccggctgtt tgccgccaac aacccccatt ccaaccttgt 351 ccgcatcgaa tacgaccgtt tcggcgaaga atacctgttc ttcattaaag 401 gctgggacaa ccaccaggca caacgcctgc ccagcccgct gtttatcccg 451 ggcctgccgc ttgccccgat ttggcacgaa ttcatcatcc tctccttcat 501 catcattgtc ggactgctga tggcatatat ccttgccggc aacattgcca 551 aacccatcag aatcttaggc aacggcatgg acagggtggc agaacgagaa 601 cttgaagacc gcgtttgcca acaggttcgc gaccgcgacg acgaattggc 651 cgatgttgcc atgcaattcg acacaatggt ggaaaaactg gaataa This corresponds to the amino acid sequence <SEQ ID 1804; ORF 585.ng>: g585.pep.. 1 MKLFQRIFAT FCAVIVCAIF VASFSFWLVQ ΝTLAEΝQFΝQ RRTIETTLMG 51 SIISAFKTRG DΝGAREILTE WKΝSPVSSAV YVIQGDEKKD ILΝRYIDΝYT 101 IERARLFAAΝ ΝPHSΝLVRIE YDRFGEEYLF FIKGWDΝHQA QRLPSPLFIP 151 GLPLAPIWHE FIILSFIIIV GLLMAYILAG ΝIAKPIRILG ΝGMDRVAERE 201 LEDRVCQQVR DRDDELADVA MQFDTMVEKL E* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1805>: m585.seq.. 1 ATGAAACTGT TCCAACGCAT TTTCGCCACA TTTTGCGCGG TTATCGTCTG 51 TGCAATCTTT GTGGCGAGTT TTTCTTTCTG GCTGGTGCAG AACACCCTTG 101 CCGAAAACCA GTTCAACCAA CGCCGCACCA TCGAAACCAC TTTGATGGGC 151 AGCATCATTT CCGCATTCCG GGCACGCGGG GACGCGGGTG CGCGCGAAAT 201 CCTGACGGAA TGGAAAGACA GCCCCGTCTC ATCGGGCGTG TACGTTATAC 251 AGGGCGACGA GAAAAAAGAT ATCCTGAACC GGTATATCGA CAGCTATACC 301 ATCGAACGCG CCCGGCTTTT CGCCGCCGGA CACCCGCATT CCAACCTCGT 351 CCATATCGAA TACGACCGCT TCGGCGAAGA ATACCTGTTC TTCACCAAAG 401 ACTGGGACAA ACTCCAAGCC CGCCGCCTGC CCAGCCCCCT GTTGATCCCC 451 GGCCTGCCGC TCGCCCCGAT TTGGCACGAA CTCATCATAT TGTCCTTCAT 501 CATCATCGTC GGACTGCTGA TGGCATATAT CCTCGCCGGC AACATTGCCA 551 AACCCATCAG AATCTTAGGC AACGGCATGG ACAGGGTGGC AAACGGAGAA 601 CTTGAAACCC GTATCTCCCA ACAGGTCGAC GACCGCGACG ACGAATTGTC 651 CCATCTTGCC ATCCAATTCG ACAAAATGGT GGAAAAACTC GAAAAACTCG 701 TTGCCAAAGA ACGCCACCTG CTCCATCACG TCTCCCATGA AATGCGTTCT 751 CCCCTTGCGC GCATGCAGGC AATTGTCGGA CTGATTCAGG CGCAGCCCCA 801 AAAACAGGAG CAATATCTCA AACGGCTGGA AGGCGAACTG ACCCGCATGG 851 ATACGCTGGC CGGGGAACTG TTAACCCTGT CCCGTCTCGA AACTTCCAAT 901 ATGGCTTTGG AAAAAGAAAG CCTGAAACTC CTGCCCTTCC TGGGCAACCT 951 GGTAGAAGAC AATCAAAGCA TTGCCCAGAA AAACGGACAA ACGGTTACCC 1001 TGTCTGCCGA CGGAAAAATC CCCGAAAACA CAACCATCCT TGCCAACGAA 1051 AGCTACCTGT ACCGCGCCTT CGACAACGTC ATCCGCAACG CCGTCAACTA 1101 CAGTCCCGAA GGCAGCACCA TCCTGATCAA CATCGGACAA GACCACAAAC 1151 ACTGGATAAT CGACGTTACC GACAACGGCC CCGGCGTGGA CGAAATGCAG 1201 CTCCCGCACA TCTTCACCGC TTTCTACCGT GCAGACTCCA GTGCCAACAA 1251 ACCCGGAACA GGACTGGGGC TTGCATTGAC CCAACATATT ATTGAACAGC 1301 ACTGCGGCAA AATCATCGCC GAAAACATCA AACCGAACGG TCTGCGGATG 1351 CGCTTTATCC TGCCCAAGAA AAAAACCGGT TCCAAAACAG AAAAAAGTGC 1401 GAACTGA This corresponds to the amino acid sequence <SEQ ID 1806; ORF 585>: m585.pep .. 1 MKLFQRIFAT FCAVIVCAIF VASFSFWLVQ NTLAENQFNQ RRTIETTLMG 51 SIISAFRARG DAGAREILTE WKDSPVSSGV YVIQGDEKKD ILNRYIDSYT 101 IERARLFAAG HPHSNLVHIE YDRFGEEYLF FTKDWDKLQA RRLPSPLLIP 151 GLPLAPIWHE LIILSFIIIV GLLMAYILAG NIAKPIRILG NGMDRVANGE 201 LETRISQQVD DRDDELSHLA IQFDKMVEKL EKLVAKERHL LHHVSHEMRS 251 PLARMQAIVG LIQAQPQKQE QYLKRLEGEL TRMDTLAGEL LTLSRLETΞN 301 MALEKESLKL LPFLGNLVED NQSIAQKNGQ TVTLSADGKI PENTTILANE 351 SYLYRAFDNV IRNAVNYSPE GΞTILINIGQ DHKHWIIDVT DNGPGVDEMQ 401 LPHIFTAFYR ADSSANKPGT GLGLALTQHI IEQHCGKIIA ENIKPNGLRM 451 RFILPKKKTG ΞKTEKΞAN* m585 / g585 88.3% identity in 231 aa overlap 10 20 30 40 50 60 m585.pep MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTIETTLMGΞIIΞAFRARG g585 MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTIETTLMGSIISAFKTRG 10 20 30 40 50 60 70 80 90 100 110 120 m585.pep DAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIERARLFAAGHPHSNLVHIE I g585 DNGAREILTEWKNSPVSSAVYVIQGDEKKDILNRYIDNYTIERARLFAANNPHSNLVRIE 70 80 90 100 110 120 130 140 150 160 170 180 m585.pep YDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHELIILSFIIIVGLLMAYILAG M M M I M : g585 YDRFGEEYLFFIKGWDNHQAQRLPSPLFIPGLPLAPIWHEFIILSFIIIVGLLMAYILAG 130 140 150 160 170 180 190 200 210 220 230 240 m585.pep NIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSHLAIQFDKMVEKLEKLVAKERHL I I I M I I I M I I I M M M M g585 NIAKPIRILGNGMDRVAERELEDRVCQQVRDRDDELADVAMQFDTMVEKLEX 190 200 210 220 230 250 260 270 280 290 300 m585.pep LHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRLEGELTRMDTLAGELLTLSRLETSN The following partial DNA sequence was identified in N meningitidis <SEQ ID 1807>: a585. seq 1 ATGAAACTGT TCCAACGCAT CTTCGCCACA TTTTGCGCGG TTATCGTCTG 51 TGCAATCTTT GTGGCGAGTT TTTCTTTCTG GCTGGTGCAG AACACCCTTG 101 CCGAAAACCA GTTCAACCAA CGCCGCACCA TCGAAACCAC TTTGATGGGC 151 AGCATCATTT CCGCATTCCG GGCACGCGGG GACGCGGGTG CGCGCGAAAT 201 CCTGACGGAA TGGAAAGACA GCCCCGTCTC ATCGGGCGTG TACGTTATAC 251 AGGGCGACGA GAAAAAAGAT ATCCTGCACC GGTATATCGA CAGCTACACC 301 ATCGAACGCG CCCGGCTTTT CGCCGCCGGA CACCCGCATT CCAACCTCGT 351 CCATATCGAA TACGACCGCT TCGGCGAAGA ATACCTGTTC TTCACCAAAG 401 ACTGGGACAA ACTCCAAGCC CGCCGCCTGC CCAGCCCCCT GTTGATCCCC 451 GGCCTGCCGC TCGCCCCGAT TTGGCACGAA CTCATCATAT TGTCCTTCAT 501 CATCATCGTC GGACTGCTGA TGGCGTACAT CCTCGCCGGC AACATTGCCA 551 AACCCATCAG AATCTTAGGC AACGGCATGG ACAGGGTGGC AAACGGAGAA 601 CTTGAAACCC GTATCTCCCA ACAGGTCGAC GACCGCGACG ACGAATTGTC 651 CCATCTTGCC ATCCAATTCG ACAAAATGGT GGAAAAACTC GAAAAACTCG 701 TTGCCAAAGA ACGCCACCTG CTCCATCACG TCTCCCATGA AATGCGTTCT 751 CCCCTTGCGC GCATGCAGGC AATTGTCGGA CTGATTCAGG CGCAGCCCCA 801 AAAACAGGAG CAATATCTCA AACGGCTGGA AGGCGAACTG ACCCGCATGG 851 ATACGCTGGC CGGGGAACTG TTAACCCTGT CCCGTCTCGA AACTTCCAAT 901 ATGGCTTTGG AAAAAGAAAG CCTGAAACTC CTGCCCTTCC TGGGCAACCT 951 GGTAGAAGAC AATCAAAGCA TTGCCCAGAA AAACGGACAA ACGGTTACCC 1001 TGTCTGCCGA CGGAAAAATC CCCGAAAACA CAACCATCCT TGCCAACGAA 1051 AGCTACCTGT ACCGCGCCTT CGACAACGTC ATCCGCAACG CCGTCAACTA 1101 CAGTCCCGAA GGCAGCACCA TCCTGATCAA CATCGGACAA GACCACAAAC 1151 ACTGGATAAT CGACGTTACC GACAACGGCC CCGGCGTGGA CGAAATGCAG 1201 CTCCCGCACA TCTTCACCGC TTTCTACCGT GCAGACTCCA GTGCCAACAA 1251 ACCCGGAACA GGACTGGGGC TTGCATTGAC CCAACATATT ATTGAACAGC 1301 ACTGCGGCAA AATCATCGCC GAAAACATCA AACCGAACGG TCTGCGGATG 1351 CGCTTTATCC TGCCCAAGAA AAAAACCGGT TCCAAAACAG AAAAAAGTGC 1401 GAACTGA This corresponds to the amino acid sequence <SEQ ID 1808; ORF 585. a>: a585.pep 1 MKLFQRIFAT FCAVIVCAIF VASFSFWLVQ NTLAENQFNQ RRTIETTLMG 51 SIISAFRARG DAGAREILTE WKDSPVSSGV YVIQGDEKKD ILHRYIDSYT 101 IERARLFAAG HPHSNLVHIE YDRFGEEYLF FTKDWDKLQA RRLPSPLLIP 151 GLPLAPIWHE LIILSFIIIV GLLMAYILAG NIAKPIRILG NGMDRVANGE 201 LETRISQQVD DRDDELSHLA IQFDKMVEKL EKLVAKERHL LHHVSHEMRS 251 PLARMQAIVG LIQAQPQKQE QYLKRLEGEL TRMDTLAGEL LTLSRLETSN 301 MALEKESLKL LPFLGNLVED NQSIAQKNGQ TVTLSADGKI PENTTILANE 351 SYLYRAFDNV IRNAVNYSPE GSTILINIGQ DHKHWIIDVT DNGPGVDEMQ 401 LPHIFTAFYR ADSSANKPGT GLGLALTQHI IEQHCGKIIA ENIKPNGLRM 451 RFILPKKKTG SKTEKSAN* m585/a585 99.8% identity in 468 aa overlap 10 20 30 40 50 60 m585. pep MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTIETTLMGS11SAFRARG I II II II II II II II I II II II II II I II II II II II I II I II II II I I I II I II I I II I a585 MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTIETTLMGSIISAFRARG 10 20 30 40 50 60 70 80 90 100 110 120 m585. pep DAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIERARLFAAGHPHSNLVHIE MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a585 DAGAREILTEWKDSPVSSGVYVIQGDEKKDILHRYIDSYTIERARLFAAGHPHSNLVHIE 70 80 90 100 110 120 130 140 150 160 170 180 m585.pep YDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHELIILSFIIIVGLLMAYILAG II II II II II II II I II I II II I II II II II II II II II II II I I II I I I II II I I I I M a585 YDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHELIILSFIIIVGLLMAYILAG 130 140 150 160 170 180 190 200 210 220 230 240 m585.pep NIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSHLAIQFDKMVEKLEKLVAKERHL I II II II II II II I II I I II II II I II II II II II II I II II I I II I I I I II I II II II I a585 NIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSHLAIQFDKMVEKLEKLVAKERHL 190 200 210 220 230 240 250 260 270 280 290 300 m585. pep LHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRLEGELTRMDTLAGELLTLSRLETSN II II II II II II I II I II II I II II I I II I I I I M I I I M I M M I I I M M M M M II a585 LHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRLEGELTRMDTLAGELLTLSRLETSN 250 260 270 280 290 300 310 320 330 340 350 360 m585.pep MALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTLSADGKIPENTTILANESYLYRAFDNV II II I II II II I II I II I I II I I I II I I I II I I I I I M I I M M I M M I I M I I I I I I I a585 MALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTLSADGKIPENTTILANESYLYRAFDNV 310 320 330 340 350 360 370 380 390 400 410 420 m585.pep IRNAVNYSPEGSTILINIGQDHKHWIIDVTDNGPGVDEMQLPHIFTAFYRADSSANKPGT II II II I II II I I II II II II I I II I II II II I I II I II II II II II II II II I I II I II a585 IRNAVNYSPEGSTILINIGQDHKHWIIDVTDNGPGVDEMQLPHIFTAFYRADSSANKPGT 370 380 390 400 410 420 430 440 450 460 469 m585.pep GLGLALTQHIIEQHCGKIIAENIKPNGLRMRFILPKKKTGSKTEKSANX II II II II I II II I II II II II I II II II II II II II II II I II II II I a585 GLGLALTQHIIEQHCGKIIAENIKPNGLRMRFILPKKKTGSKTEKSANX 430 440 450 460 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1809>: g586.seq.. 1 atggcagccc atctcgaaga acaacaagag ttagacaact ttaaatattt 51 ttggaaaacc acgggcaaat ggctgtttgc cctgctgatt ttggcggcac 101 tcggctactt gggatacacg gtttaccaaa accgtgcggc ttcccaaaat 151 caggaagcgg cggcggtgct ggcaaacatc gtggaaaagg cgcaaaacaa 201 agccccgcaa agcgaaatca atgccgaact gtccaaactc caacaaagct 251 acccccattc catttccgcc gcccaagcca cgctgatggc ggcggcaacc 301 gaatttgacg cgcagcgtta cgatgttgcc gaaggtcatt tgaaatgggt 351 gttgtccaac caaaaagaca gcctgattca ggcgttggcg gcgcagcgtc 401 tgggcgttgt gttgttgcaa caaaaaaaat acgatgccgc gcttgccgca 451 ctcgacacgc cggttgaggc ggacttcgcc cccctgctga tggaaactaa 501 aggcgatgtt tatgccgcac aggaaaaaag ccaggaagcc ttaaaaaact 551 acggacaggc tttggaaaaa atgcctcaag attctgtcgg tcgcgaattg 601 cttcaaatga aactcgattc gctgaaataa This corresponds to the amino acid sequence <SEQ ID 1810; ORF 586.ng>: g586.pep.. 1 MAAHLEEQQE LDΝFKYFWKT TGKWLFALLI LAALGYLGYT VYQΝRAASQΝ 51 QEAAAVLAΝI VEKAQΝKAPQ SEIΝAELSKL QQSYPHSISA AQATLMAAAT 101 EFDAQRYDVA EGHLKWVLSΝ QKDSLIQALA AQRLGWLLQ QKKYDAALAA 151 LDTPVEADFA PLLMETKGDV YAAQEKSQEA LKΝYGQALEK MPQDSVGREL 201 LQMKLDSLK* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1811>: m586.seq 1 ATGGCAGCCC ATCTCGAAGA ACAACAAGAG TTAGACAACT TTAAATATTT 51 TTGGAAAACC ACGGGCAAAT GGCTGTTTGC CTTGCTGATT TTGGCGGCAC 101 TCGGCTACTT GGGATACACG GTTTACCAAA ACCGTAAAGT TTCCCAAAAT 151 CAGGAAGCGG CGGCGGTGCT GGCAAACATC GTAGAAAAGG CGCAAAGCAA 201 AGCCCCGCAA AGCGAAATCA ATGCCGAATT GACCAAACTC CAACAAAGCT 251 ACCCGCATTC CATTTCCGCC GCCCAAGCCA CACTGATGGC GGCGGCAACC 301 GAATTTGACG CGCAGCGTTA CGATGTTGCC GAAGGCCATT TGAAATGGGT 351 GTTGTCCAAC CAAAAAGACA GCCTGATTCA AGCGTTGGCG GCGCAGCGTC 401 TGGGCGTTGT GTTGTTGCAA CAAAAAAAAT ACGATGCCGC GCTTGCCGCG 451 CTCGATACGC CGGTTGAAGC GGACTTCGCC CCCCTGCTGA TGGAAACCAA 501 AGGCGATGTC TATGCCGCAC AGGGAAAAAG CCAGGAAGCC TTAAAAAACT 551 ACGGACAGGC TTTAGAAAAA ATGCCTCAAG ATTCTGTCGG TCGCGAATTG 601 GTTCAAATGA AACTTGATTC GCTGAAATAA This corresponds to the amino acid sequence <SEQ ID 1812; ORF 586>: m586.pep 1 MAAHLEEQQE LDΝFKYFWKT TGKWLFALLI LAALGYLGYT VYQΝRKVSQΝ 51 QEAAAVLAΝI VEKAQSKAPQ SEIΝAELTKL QQSYPHSISA AQATLMAAAT 101 EFDAQRYDVA EGHLKWVLSΝ QKDSLIQALA AQRLGWLLQ QKKYDAALAA 151 LDTPVEADFA PLLMETKGDV YAAQGKSQEA LKΝYGQALEK MPQDSVGREL 201 VQMKLDSLK* m586 / g586 97.1% identity in 209 aa overlap 10 20 30 40 50 60 m586.pep MAAHLEEQQELDΝFKYFWKTTGKWLFALLILAALGYLGYTVYQΝRKVΞQΝQEAAAVLAΝI 1 1 [ M 1 1 1 1 1 1 1 1 M 1 1 1 1 1 M I M 1 1 1 1 1 1 ! i I M 1 1 1 1 1 1 1 M M I I I I M I I M M g586 MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQNRAASQNQEAAAVLANI 10 20 30 40 50 60 70 80 90 100 110 120 m586.pep VEKAQSKAPQSEINAELTKLQQSYPHSISAAQATLMAAATEFDAQRYDVAEGHLKWVLSN i 1 1 1 1 : 1 1 1 1 1 1 1 1 1 1 1 ^ [ ! i ! i 1 1 1 ! M 1 1 1 1 1 1 ! 1 1 1 1 1 1 1 1 1 1 1 i 1 1 i 1 1 1 1 1 j 1 1 1 g586 VEKAQNKAPQSEINAELSKLQQSYPHΞISAAQATLMAAATEFDAQRYDVAEGHLKWVLSN 70 80 90 100 110 120 130 140 150 160 170 180 m586 . pep QKDSLIQALAAQRLGWLLQQKKYDAALAALDTPVEADFAPLLMETKGDVYAAQGKSQEA i 1 1 1 1 1 1 1 1 1 ! i i 1 1 1 1 1 1 1 ! I ! 1 1 E ! 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 ! ! I ! i I ! 1 1 M i l l g586 QKDSLIQALAAQRLGWLLQQKKYDAALAALDTPVEADFAPLLMETKGDVYAAQEKSQEA 130 140 150 160 170 180 190 200 210 m586. pep LKNYGQALEKMPQDSVGRELVQMKLDSLKX M i n I I I I I I i M i i i i i M I I I M I I ΓI g586 LKNYGQALEKMPQDSVGRELLQMKLDSLKX 190 200 210 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1813>: a586. seq 1 ATGGCAGCCC ATTTGGAAGA ACAACAAGAG TTGGACAACT TTAAATATTT 51 TTGGAAAACC ACGGGCAAAT GGCTGTTTGC CGTGCTGATT TTGGCGGCAC 101 TCGGCTACTT GGGATACACG GTTTACCAAA ACCGTGCGGC TTCCCAAAAT 151 CAGGAAGCGG CGGCGGTGCT GGCAAACATC GTGGAAAAGG CGCAAAACAA 201 AGCCCCGCAA AGCGAAATCA ATGCCGAATT GGCCAAGCTC CAACAAAGCT 251 ACCCCCATTC CATTTCCGCC GCCCAAGCCA CGCTGATGGC GGCAGCAACC 301 GAATTTGACG CGCAGCGTTA CGATGTTGCC GAAGGCCATT TGAAATGGGT 351 ATTGTCCAAC CAAAAAGACA GCCTGATCCA GGCGTTGGCG GCGCAGCGTC 401 TGGGCGTTGT GTTGTTGCAA CAAAAAAAAT ACGATGCCGC GCTTGCCGCA 451 CTCGACACGC CGGTTGAAGC GGACTTCGCC CCCCTGCTGA TGGAAACCAA 501 AGGCGATGTC TATGCCGCAC AGGGAAAAAG CCAGGAAGCC TTAAAAAACT 551 ACGGACAGGC TTTAGAAAAA ATGCCTCAAG ATTCTGTCGG TCGCGAATTG 601 GTTCAAATGA AACTTGATTC GCTGAAATAA This corresponds to the amino acid sequence <SEQ ID 1814; ORF 586. a>: a586.pep 1 MAAHLEEQQE LDΝFKYFWKT TGKWLFAVLI LAALGYLGYT VYQΝRAASQΝ 51 QEAAAVLAΝI VEKAQΝKAPQ SEIΝAELAKL QQSYPHSISA AQATLMAAAT 101 EFDAQRYDVA EGHLKWVLSΝ QKDSLIQALA AQRLGWLLQ QKKYDAALAA 151 LDTPVEADFA PLLMETKGDV YAAQGKSQEA LKΝYGQALEK MPQDSVGREL 201 VQMKLDSLK* m586/a586 97.6% identity in 209 aa overlap 10 20 30 40 50 60 m586 .pep MAAHLEEQQELDΝFKYFWKTTGKWLFALLILAALGYLGYTVYQΝRKVSQΝQEAAAVLAΝI II II II I II I II II I I I II II II I I II : II II II II II II II II I : II II II I I II I I I a586 MAAHLEEQQELDΝFKYFWKTTGKWLFAVLILAALGYLGYTVYQΝRAASQΝQEAAAVLAΝI 10 20 30 40 50 60 70 80 90 100 110 120 m586.pep VEKAQSKAPQSEIΝAELTKLQQSYPHSISAAQATLMAAATEFDAQRYDVAEGHLKWVLSΝ II II I : I II II I I II II : I II I I I I II II II I I II II II I I I I I II I II II I I I I I I I I I a586 VEKAQΝKAPQSEIΝAELAKLQQSYPHSISAAQATLMAAATEFDAQRYDVAEGHLKWVLSΝ 70 80 90 100 110 120 130 140 150 160 170 180 m586. pep QKDSLIQALAAQRLGWLLQQKKYDAALAALDTPVEADFAPLLMETKGDVYAAQGKSQEA I II II II II II I I I II II II I I I I II I I I I I M I I I I M I I M I I I M M M I I I I I I I I a586 QKDSLIQALAAQRLGWLLQQKKYDAALAALDTPVEADFAPLLMETKGDVYAAQGKSQEA 130 140 150 160 170 180 190 200 210 m586.pep LKNYGQALEKMPQDSVGRELVQMKLDSLKX II II II II II II II II II II II II II II II a586 LKNYGQALEKMPQDSVGRELVQMKLDSLKX 190 200 210 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1815>: g587. seq.. 1 atgaaacgta tctttttgcc cgccttgccc gccatcctgc ctttatccgc 51 ttatgccgac ctgcccttga cgattgaaga cataatgacc gacaagggaa 101 aatggaaact ggaaacttcc cttacctatc tgaatagcga aaacagccgc 151 gccgcacttg ccgcaccggt ttacattcaa accggcgcaa cctcgtttat 201 ccccattccg accgaaattc aagaaaacgg cagcaatacc gatatgctcg 251 ccggcacgct cggtttgcgc tacggactga ccggcaatac cgacatttac 301 ggcagcggca gctatctgtg gcacgaagaa cgcaaactcg acggcaacgg 351 caaaacccgc aacaaacgga tgtccgacat atccgccggc atcagccaca 401 ccttccttaa agacggcaaa aaccccgccc taatcagctt tcttgaaagc 451 acggtttacg aaaaatcgcg caacaaagcc tcgttaatca aaaaaagggg 501 gctttgcccc ttttataact taaggataaa ttatgaatat taa This corresponds to the amino acid sequence <SEQ ID 1816; ORF 587.ng>: g587.pep.. 1 MKRIFLPALP AILPLSAYAD LPLTIEDIMT DKGKWKLETS LTYLΝSEΝSR 51 AALAAPVYIQ TGATSFIPIP TEIQEΝGSΝT DMLAGTLGLR YGLTGΝTDIY 101 GSGSYLWHEE RKLDGΝGKTR ΝKRMSDISAG ISHTFLKDGK ΝPALISFLES 151 TVYEKSRΝKA SLIKKRGLCP FYΝLRIΝYEY * The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1817>: m587. seq.. 1 ATGAAGCGCA TCTTTTTGCC CGCCTTGCCC GCCATCCTGC CTTTATCCAC 51 TTATGCCGAC CTGCCCTTGA CGATTGAAGA CATAATGACC GACAAGGGAA 101 AATGGAAACT GGAAACTTCC CTTACCTACC TGAACAGCGA AAACAACCGC 151 GCCGAACTTG CCGCACCGGT TTACATTCAA ACCGGCGCAA CCTCGTTTAT 201 CCCCATTCCG ACCGAAATCC AAGAAAACGG CAGCAATACC GATATGCTCG 251 TCGGCACGCT CGGTTTGCGC TACGGACTGA CCGGGAATAC CGACATTTAC 301 GGCAGCGGCA GCTATCTGTG GCACGAAGAA CGCAAACTCG ACGGCAACAG 351 CAAAACCCGC AACAAACGGA TGTCCGACGT ATCCCTCGGC ATCAGCCACA 401 CTTTCCTTAA AGACGACAAA AACCCCGCCC TAATCAGCTT TCTTGAAAGC 451 ACGGTTTACG AAAAATCGCG CAACAAAGCC TCGTCGGGAA AATCCTGGCT 501 CATCGGCGCC ACCACCTACA AAGCCATAGA TCCGATTGTC CTTTCCCTCA 551 CCGCCGCCTA CCGCATCAAC GGCAGCAAAA CCCTTTCAGA CGGCATCCGC 601 TACAAATCGG GCAACTACCT GCTGCTCAAC CCCAACATCT CATTTGCTGC 651 CAACGACAGA ATCAGCCTGA CCGGAGGCAT CCAATGGCTG GGCAGGCAGC 701 CCGACCGGAC GGACGGCAAA CGGGAATCCT CCAGAAACAC ATCCACCTAC 751 GCCCATTTCG GCGCAGGTTT CGGTTTCACC AAAACCACGG CTTTAAACGC 801 ATCCGCACGT TTCAACGTTT CAGGGCAAAG CAGTTCCGAA CTGAAATTTG 851 GCGTACAGCA TACATTTTAA This corresponds to the amino acid sequence <SEQ ID 1818; ORF 587>: m587.pep.. 1 MKRIFLPALP AILPLSTYAD LPLTIEDIMT DKGKWKLETS LTYLΝSEΝΝR 51 AELAAPVYIQ TGATSFIPIP TEIQEΝGSΝT DMLVGTLGLR YGLTGΝTDIY 101 GSGSYLWHEE RKLDGΝSKTR ΝKRMSDVSLG ISHTFLKDDK ΝPALISFLES 151 TVYEKSRΝKA SSGKSWLIGA TTYKAIDPIV LSLTAAYRIΝ GSKTLSDGIR 201 YKSGΝYLLLΝ PΝISFAAΝDR ISLTGGIQWL GRQPDRTDGK RESSRΝTSTY 251 AHFGAGFGFT KTTALΝASAR FΝVSGQSSSE LKFGVQHTF* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m587 / g587 95.0% identity in 161 aa overlap 10 20 30 40 50 60 m587.pep MKRIFLPALPAILPLSTYADLPLTIEDIMTDKGKWKLETSLTYLNSENNRAELAAPVYIQ I II II I II I I II II II : I II II II II II II II I II II II II I II II II : II llllllll g587 MKRIFLPALPAILPLSAYADLPLTIEDIMTDKGKWKLETSLTYLNSENSRAALAAPVYIQ 10 20 30 40 50 60 70 80 90 100 110 120 m587. pep TGATSFIPIPTEIQENGSNTDMLVGTLGLRYGLTGNTDIYGSGSYLWHEERKLDGNSKTR MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g587 TGATSFIPIPTEIQENGSNTDMLAGTLGLRYGLTGNTDIYGSGSYLWHEERKLDGNGKTR 70 80 90 100 110 120 130 140 150 160 170 180 m587. pep NKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKASSGKSWLIGATTYKAIDPIV II II II : I II II II II I II II I II II II II II I II II II g587 NKRMSDISAGISHTFLKDGKNPALISFLESTVYEKSRNKASLIKKRGLCPFYNLRINYEY 130 140 150 160 170 180 190 200 210 220 230 240 m587. pep LSLTAAYRINGSKTLSDGIRYKSGNYLLLNPNISFAANDRISLTGGIQWLGRQPDRTDGK g587 X The following partial DNA sequence was identified in N meningitidis <SEQ ID 1819>: a587. seq 1 ATGAAGCGCA TCTTTTTGCC CGCCTTGCCC GCCATCCTGC CTTTATCCGC 51 TTATGCCGAC CTGCCCTTGA CGATTGAAGA CATAATGACC GACAAGGGCA 101 AATGGAAACT GGAAACTTCC CTTACCTACC TGAACAGCGA AAACAACCGC 151 GCCGAACTTG CCGCACCGGT TTACATCCAA ACCGGCGCAA CCTCGTTTAT 201 CCCCATTCCG ACCGAAATCC AAGAAAACGG CAGCAATACC GATATGCTCG 251 TTGGCACGCT CGGTTTGCGC TACGGACTGA CCGGGAATAC CGACATTTAC 301 GGCAGCGGCA GCTATCTGTG GCACGAAGAA CGCAAACTCG ACGGCAACGG 351 CAAAACCCGA AACAAACGGA TGTCCGACGT ATCCCTCGGC ATCAGCCACA 401 CCTTCCTTAA AGACGACAAA AACCCCGCCC TAATCAGCTT TCTTGAAAGC 451 ACGGTTTACG AAAAATCGCG CAACAAAGCC TCGTCGGGAA AATCCTGGCT 501 CATCGGCGCC ACCACCTACA AAGCCATCGA CCCCGTCGTC CTCTCATTGA 551 CCGCTGCCTA CCGTATCAAC GGCAGCAAAA CCCTTTCAAG CAACACCAAA 601 TACAAAGCAG GCAATTACTG GATGCTGAAT CCCAATATAT CCTTCGCCGC 651 CAACGACAGA ATCAGCCTCA CGGGCGGCAT CCAATGGCTG GGCAAGCAGC 701 CCGACCGTCT GGACGGCAAA AAAGAATCCG CAAGAAACAC ATCCACCTAT 751 GCCCATTTCG GCGCAGGTTT CGGTTTCACC AAAACCACGG CTTTAAACGC 801 ATCCGCACGT TTCAACGTTT CAGGGCAAAG CAGTTCCGAA CTGAAATTTG 851 GCGTACAGCA TACGTTTTAA This corresponds to the amino acid sequence <SEQ ID 1820; ORF 587.a>: a58 .pep 1 MKRIFLPALP AILPLSAYAD LPLTIEDIMT DKGKWKLETS LTYLΝSEΝΝR 51 AELAAPVYIQ TGATSFIPIP TEIQEΝGSΝT DMLVGTLGLR YGLTGΝTDIY 101 GSGSYLWHEE RKLDGΝGKTR ΝKRMSDVSLG ISHTFLKDDK ΝPALISFLES 151 TVYEKSRΝKA SSGKSWLIGA TTYKAIDPW LSLTAAYRIΝ GSKTLSSΝTK 201 YKAGΝYWMLΝ PΝISFAAΝDR ISLTGGIQWL GKQPDRLDGK KESARΝTSTY 251 AHFGAGFGFT KTTALΝASAR FΝVSGQSSSE LKFGVQHTF* m587/a587 95.2% identity in 289 aa overlap 10 20 30 40 50 60 m587. pep MKRIFLPALPAILPLSTYADLPLTIEDIMTDKGKWKLETSLTYLΝSEΝΝRAELAAPVYIQ MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a587 MKRIFLPALPAILPLSAYADLPLTIEDIMTDKGKWKLETSLTYLΝSEΝΝRAELAAPVYIQ 10 20 30 40 50 60 70 80 90 100 110 120 m587. pep TGATSFIPIPTEIQEΝGSΝTDMLVGTLGLRYGLTGΝTDIYGSGSYLWHEERKLDGΝSKTR MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a587 TGATSFIPIPTEI Q EΝGSΝTDMLVGTLGLRYGLTGΝTDIYGSGSYLWHEERKLDGΝGKTR 70 80 90 100 110 120 130 140 150 160 170 180 m587. pep NKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKASSGKSWLIGATTYKAIDPIV MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a587 NKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKASSGKSWLIGATTYKAIDPW 130 140 150 160 170 180 190 200 210 220 230 240 m587. pep LSLTAAYRINGSKTLSDGIRYKSGNYLLLNPNISFAANDRISLTGGIQWLGRQPDRTDGK I I I I I I I I I I I I I I I I : : M M M M M M M M M M M M I M M M I I I a587 LSLTAAYRINGSKTLSSNTKYKAGNYWMLNPNISFAANDRISLTGGIQWLGKQPDRLDGK 190 200 210 220 230 240 250 260 270 280 290 m587.pep RESSRNTSTYAHFGAGFGFTKTTALNASARFNVSGQΞSSELKFGVQHTFX : I I : I I I I II II II II II II II I II II I II I I II II II II II I II II I II a587 KESARNTSTYAHFGAGFGFTKTTALNASARFNVSGQSSSELKFGVQHTFX 250 260 270 280 290 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1821>: g588. seq 1 atgcttaaac atctcgcatt cctactgccc gccatgatgt tcgccctccc 51 cgcccagacc gccgtcctaa gcccctatca ggaaaccggc tgcacctacg 101 aaggcgggat cggaaaagac gggcttcctt caggcaaagg catatggcgt 151 tgccgggatg ggcgcggtta taccggttca ttcaaaaacg gcaaattcga 201 cgggcaaggc gtttataccg ttgccgccgg ccgcgaagta tttctcgagc 251 cgttcaattc cgacagtacc aaattccgca atatggcatt gtcgggcacg 301 ttcaaacaag gcttggcaca cggcaggttc gccgcctcgc aaaacggcga 351 aaccctcttt tattatgaaa tgcgaacacg gcatgattaa This corresponds to the amino acid sequence <SEQ LD 1822; ORF 588.ng>: g588.pep.. 1 MLKHLAFLLP AMMFALPAQT AVLSPYQETG CTYEGGIGKD GLPSGKGIWR 51 CRDGRGYTGS FKΝGKFDGQG VYTVAAGREV FLEPFΝSDST KFRΝMALSGT 101 FKQGLAHGRF AASQΝGETLF YYEMRTRHD* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1823>: m588. seq.. 1 ATGCTTAAAC ATCTCGCATT CCTACTGCCC GCCATGATGT TCGCCCTCCC 51 CACTTCGGCC GCCGTCCTGA CTTCCTATCA AGAACCAGGC TGCACCTACG 101 ACGGCAATGT CGGCAAAGAC GGTAAACCCG CCGGCAAAGG CACATGGCGC 151 TGCCAAGACG GGCGCAACTA TACCGGTTCG TTTAAAAACG GCAAATTCGA 201 CGGGCAAGGC GTTTATACCG TTGCCGCCAA CCGCGAAATA TTTATCGAAC 251 CGTTCAATTC CGACAGTACC AAATTCCGCA ACATGGTACT CTCGGGCACG 301 TTCAAAAAAG GCTTGGCACA CGGCAGATTT ACCGTCTCGC AAAACGGCGA 351 AACCCTCTTC ATTATGAAAT GCGAAAACGG CATGATTAAA GAAGTGAAAC 401 TGCCCAAAAA CAAATAA This corresponds to the amino acid sequence <SEQ ID 1824; ORF 588>: m588.pep.. 1 MLKHLAFLLP AMMFALPTSA AVLTSYQEPG CTYDGΝVGKD GKPAGKGTWR 51 CQDGRΝYTGS FKΝGKFDGQG VYTVAAΝREI FIEPFΝSDST KFRΝMVLSGT 101 FKKGLAHGRF TVSQΝGETLF IMKCEΝGMIK EVKLPKΝK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m588 / g588 82.5% identity in 120 aa overlap 10 20 30 40 50 60 m588.pep MLKHLAFLLPAMMFALPTSAAVLTSYQEPGCTYDGNVGKDGKPAGKGTWRCQDGRNYTGS I II I I I II I I I II I II I ::: I I I : III II II : I : : I II I Mill II I : II I : II I I g588 MLKHLAFLLPAMMFALPAQTAVLSPYQETGCTYEGGIGKDGLPSGKGIWRCRDGRGYTGS 10 20 30 40 50 60 70 80 90 100 110 120 m588. pep FKNGKFDGQGVYTVAANREIFIEPFNSDSTKFRNMVLSGTFKKGLAHGRFTVSQNGETLF I II I II I I I II I II I I : II : I : I II II II II II II : II I I I I M II II I I : : I I I II I I I g588 FKNGKFDGQGVYTVAAGREVFLEPFNSDSTKFRNMALSGTFKQGLAHGRFAASQNGETLF 70 80 90 100 110 120 130 139 m588.pep IMKCENGMIKEVKLPKNKX g588 YYEMRTRHDX 130 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1825>: a588. seq 1 ATGCTTAAAC ATCTCGCATT CCTACTGCCC GCCATGATGT TCGCCCTCCC 51 CGCCGCGTCC GCCGTTCTGA CTTCCTATCA AGAACCCGGC TGCACCTACG 101 AAGGCGATGT CGGCAAAGAC GGTAAACCCG CCGGCAAAGG CACATGGCGC 151 TGCCAAGACG GGCGCAACTA TACCGGTTCG TTTAAAAATG GCAAATTCGA 201 CGGACAAGGC GTTTATACCG TTGCCGCCAA CCGCGAAATA TTTATCGAAC 251 CGTTCAATTC CGACAGTACC AAATTCCGCA ACATGGTACT CTCGGGCACA 301 TTCAAAAAAG GCTTGGCACA CGGCAGATTT ACCGTCTCGC AAAACGGCGA 351 AACCCTCTTC ATTATGAAAT GCGAAAACGG CATGATTAAA GAAGTGAAGC 401 TGCCCAAAAA CAAATAA This corresponds to the amino acid sequence <SEQ ID 1826; ORF 588.a>: a588.pep 1 MLKHLAFLLP AMMFALPAAS AVLTSYQEPG CTYEGDVGKD GKPAGKGTWR 51 CQDGRΝYTGS FKΝGKFDGQG VYTVAAΝREI FIEPFΝSDST KFRΝMVLSGT 101 FKKGLAHGRF TVSQΝGETLF IMKCEΝGMIK EVKLPKΝK* m588 'a58 96.4% identity in 138 aa overlap 10 20 30 40 50 60 m588. pep MLKHLAFLLPAMMFALPTSAAVLTSYQEPGCTYDGΝVGKDGKPAGKGTWRCQDGRΝYTGS II I I II II I II II I II I :: : II I II I I II II II : I : I II II II II II II II II II II II I a588 MLKHLAFLLPAMMFALPAASAVLTSYQEPGCTYEGDVGKDGKPAGKGTWRCQDGRΝYTGS 10 20 30 40 50 60 70 80 90 100 110 120 m588.pep FKΝGKFDGQGVYTVAAΝREIFIEPFΝSDSTKFRΝMVLSGTFKKGLAHGRFTVSQΝGETLF II II I II I II II I II II II II II II I II II I II I II II II II II I II I II II II I I I I I I a588 FKΝGKFDGQGVYTVAAΝREIFIEPFΝSDSTKFRΝMVLSGTFKKGLAHGRFTVSQΝGETLF 70 80 90 100 110 120 130 139 m588.pep IMKCENGMIKEVKLPKNKX II I II II II II II II I II I a588 IMKCENGMIKEVKLPKNKX 130 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1827>: g589. seq. 1 atgcaacaaa aaatccgttt ccaaatcgag gcgatgacct gtcaggcatg 51 tgcttcgcgc attgaaaaag tgttgaacaa aaaagatttt gtcgaatcgg 101 cgggagtgaa ctttgccagt gaggaagcgc aggttacgtt tgacggcagc 151 aaaacctcgg ttgccgacat tgccaaaatc attgagaaaa ccggttacgg 201 cgcgaaggaa aaaacggaag atacattgcc gcaacctgaa gcagaacacc 251 atatcggctg gcggttgtgg cttttgctga ccatcaatat cccgttcctt 301 atcggtatgg tagggatgat gctaaaaggg ctgaattgga cacggcacga 351 ttggatgatt ccgcctgtat ggcagtttgt actggcaagc atagtgcaac 401 tttggctggc aatcccgttt tacaaaagcg cgtgggcaag cattaaaggc 451 gggctggcga atatggacgt actcgttacc atcggcacgg tgtcgattta 501 cctgtattcc gtttatatgc tgtttttcag ttcgcatgcg gcgcacggta 551 tggcgcatgt gtattttgaa gcgggcgtga tggtgatcgg ttttgtgtcg 601 ctgggtaagt ttttggaaca ccgcaccaaa aaatccagcc tgaacagctt 651 gggcttactg ctaaaactca cgccgaccca agtcaacgtg caacgcaacg 701 gcgaatggaa acaactgccc atcgaccaag tgcaaatcgg cgaccttatc 751 cgcaccaacc acggcgaacg catcgctgcc gacggcatta tcgaaagcgg 801 cagcggttgg gcggacgaaa gccaccttac cggcgaatcc aatcccgaag 851 agaaaaaggc gggcggcaaa gtgttggcgg gcgcgctgat gaccgaaggc 901 agcgtggtgt accgcgccgc gcagctcggc agccaaaccc tgctcggcga 951 catgatgaac gcgctctctg aagcacaagg cagtaaagca ccgattgcgc 1001 gcgtggccga taaagcggcg gcggtatttg tgccaactgt cgtgggcatc 1051 gcgcttctga cttttatcgt tgcttggctg attaagggcg attggacggt 1101 cgcactgatg cacgccgttg ccgttttggt gattgcctgc ccgtgcgcgc 1151 tcggtctggc gacccctgcc gcgattatgg tcggcatggg caaagcggtg 1201 aaacacggca tttggtttaa agacgcggcg gcaatggagg aagcagccca 1251 cgtcgatgcc gtcgtattgg acaaaaccgg tacgctgacc gaaggcaggc 1301 cgcaggttgc cgccgtttat tacgttcccg acagcggctt tgacgaagac 1351 gctttgtacc gcatcgccgc cgccgtcgag caaaacgccg cccacccgct 1401 cgcccgcgcc atcgtctccg ccgcacaagc gcgcggtttg gagattcccg 1451 ctgcacaaaa tgcgcaaacc gttgtcggag caggcattac cgccgaagtg 1501 gaaggcgtgg gtttggtgaa atcaggcaaa gccgaatttg ccgaactgac 1551 cttgccgaag ttttcagacg gcgtttggga aatcgccagt gcggttaccg 1601 tatctgtaaa cggcaaaccg atcggcgcat tcgcactctc cgacgcgttg 1651 aaagccgata ccgccgaagc cataggccgt ctgaaaaaac acaatatcga 1701 tgtctatatt atgagcggcg ataaccaaag tacggtcgaa tacgtcgcca 1751 aacaactggg catcgcacac gccttcggta atatgagtcc gtgcgacaaa 1801 gccgccgaag tgcagaaact caaagccgcc ggcaaaaccg tggcgatggt 1851 cggcgacggc atcaacgacg cgcccgcgct tgccgccgcc aacgtcagct 1901 tcgccatgaa aggcggtgcg gacgttgccg aacacaccgc ctccgccacg 1951 ctgatgcagc attcggtcaa tcagctcgcc gatgccctgc tgatatcgca 2001 ggcaacgttg gaaaacatca agcaaaacct atttttcgcc ttcttctaca 2051 atatattggg cattccgctc gccgcgctcg gctttttaaa tcccgtcata 2101 gcaggcgcgg caatggcggc aagctcggtt tcggtattgg gcaatgccct 2151 gcgcctgaaa tgggtaaaaa tcgattga This corresponds to the amino acid sequence <SEQ ID 1828; ORF 589.ng>: g589.pep.. 1 MQQKIRFQIE AMTCQACASR IEKVLNKKDF VESAGVNFAS EEAQVTFDGS 51 KTSVADIAKI IEKTGYGAKE KTEDTLPQPE AEHHIGWRLW LLLTINIPFL 101 IGMVGMMLKG LNWTRHDWMI PPVWQFVLAS IVQLWLAIPF YKSAWASIKG 151 GLANMDVLVT IGTVSIYLYS VYMLFFSSHA AHGMAHVYFE AGVMVIGFVS 201 LGKFLEHRTK KSSLNSLGLL LKLTPTQVNV QRNGEWKQLP IDQVQIGDLI 251 RTNHGERIAA DGIIESGSGW ADESHLTGES NPEEKKAGGK VLAGALMTEG 301 SWYRAAQLG SQTLLGDMMN ALSEAQGSKA PIARVADKAA AVFVPTWGI 351 ALLTFIVAWL IKGDWTVALM HAVAVLVIAC PCALGLATPA AIMVGMGKAV 401 KHGIWFKDAA AMEEAAHVDA WLDKTGTLT EGRPQVAAVY YVPDSGFDED 451 ALYRIAAAVE QNAAHPLARA IVSAAQARGL EIPAAQNAQT WGAGITAEV 501 EGVGLVKSGK AEFAELTLPK FSDGVWEIAS AVTVSVNGKP IGAFALSDAL 551 KADTAEAIGR LKKHNIDVYI MSGDNQSTVE YVAKQLGIAH AFGNMSPCDK 601 AAEVQKLKAA GKTVAMVGDG INDAPALAAA NVSFAMKGGA DVAEHTASAT 651 LMQHSVNQLA DALLISQATL ENIKQNLFFA FFYNILGIPL AALGFLNPVI 701 AGAAMAASSV SVLGNALRLK WVKID* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1829>: m589. seq. 1 ATGCAACAAA AAATCCGTTT CCAAATCGAA GGCATGACCT GCCAGGCCTG 51 CGCTTCGCGC ATTGAAAAAG TGTTGAACAA AAAAGATTTT GTCGAATCGG 101 CGGGGGTAAA CTTCGCCAGC GAAGAGGCGC AGGTAGTGTT TGACGACAGC 151 AAAACCTCAG TAGCCGACAT TGCCAAAATC ATTGAGAAAA CCGGTTACGG 201 CGCGAAGGAA AAAACGGAAG ATACATTGCC GCAACCCGAA GCAGAACACC 251 ATATCGGCTG GCGGCTGTGG CTGCTGTTCA CCATCAACGT CCCGTTCCTT 301 ATCGGCATGG CGGGGATGAT GATCGGCAGA CACGATTGGA TGATTCCGCC 351 GTTGTGGCAG TTCGCATTGG CAAGCGTGGT GCAGCTTTGG CTGGCAATCC 401 CGTTTTACAA AAGCGCGTGG GCGAGCATTA AGGGCGGACT GGCGAATATG 451 GACGTGCTGG TTACCATCGG CACGGTCTCG ATTTACCTGT ATTCCGTCTA 501 TATGCTGTTT TTCAGCCCGC ACGCGGCGTA CGGTATGGCG CATGTGTATT 551 TTGAAGTGGG CGTGATGGTG ATCGGTTTTG TGTCACTGGG TAAATTTTTG 601 GAACACCGTA CCAAAAAATC CAGCCTCAAC AGCTTGGGCT TGCTGCTCAA 651 ACTTACACCA ACCCAAGTCA ACGTGCAACG CAACGGCGAA TGGAAACAGC 701 TTCCCATCGA CCAAGTGCAA ATCGGCGACC TTATCCGCGC CAACCACGGC 751 GAACGCATTG CCGCAGACGG CATCATTGAA AGCGGCAGCG GTTGGGCGGA 801 CGAGAGCCAT CTTACCGGCG AATCCAATCC TGAAGAAAAA AAGGCGGGCG 851 GCAAAGTGTT GGCGGGCGCG TTAATGACCG AAGGCAGTGT GGTGTACCGC 901 GCCACGCAGC TCGGCAGCCA AACCCAGCTC GGCGACATGA TGAACGCGCT 951 CTCTGAAGCA CAAGGCAGTA AAGCACCGAT TGCGCGCGTA GCCGATAAAG 1001 CGGCTGCGGT ATTCGTGCCT GCCGTCGTGG GCATTGCGTT GTTGACTTTT 1051 ATTGTTACTT GGCTGATTAA GGGCGATTGG ACGGTTGCGC TGATGCACGC 1101 CGTCGCCGTT TTGGTGATTG CCTGCCCGTG CGCGCTGGGT CTGGCAACCC 1151 CTGCCGCGAT TATGGTCGGT ATGGGCAAAG CGGTTAAACA CGGTATTTGG 1201 TTTAAAGACG CGGCAGCAAT GGAGGAAGCC GCCCACGTCG ATGCCGTCGT 1251 GTTGGACAAA ACCGGTACGC TGACCGAAGG CAGCCCGCAG GTTGCCGCCG 1301 TTTATTGCGT TCCCGACAGC GGCTTTGACG AAGACGCTTT GTACCGCATC 1351 GCCGCCGCCG TCGAACAAAA CGCCGCCCAT CCGCTCGCCC GTGCCATCGT 1401 CTCCGCCGCC CAAGCGCGCG GTTTGGACAT TCCCGCCGCA CAAAACGCAC 1451 AAACCGTTGT CGGCGCAGGC ATTACCGCCG AAGTGGAAGG CGTGGGTTTG 1501 GTGAAAGCAG GCAAAGCCGA ATTTGCCGAA CTGGCCTTGC CGAAGTTTTT 1551 AGACGGCGTT TGGGATATTG CAAGCATTGT TGCGGTCTCA GTCGATAACA 1601 AACCCATCGG CGCATTCGCA CTTGCCGACG CGTTGAAAGC CGATACCGCC 1651 GAAGCCATAG GCCGTCTGAA AAAACACAAT ATCGATGTCT ATATTATGAG 1701 CGGCGACAAC CAAGGCACGG TCGAATACGT CGCCAAACAA CTGGGCATCG 1751 CACACGCCTT CGGCAACATG AGTCCGCGCG ATAAAGCTGC CGAAGTGCAA 1801 AAACTCAAAG CCGCCGGCAA AACCGTGGCG ATGGTCGGCG ACGGCATCAA 1851 CGACGCGCCC GCGCTTGCCG CCGCTAACGT CAGCTTCGCC ATGAAAGGCG 1901 GAGCGGACGT TGCCGAACAT ACCGCATCCG CCACGCTGAT GCAGCATTCG 1951 GTCAACCAAC TCGCCGATGC TCTGCTGGTG TCGCAAGCCA CTTTGAAAAA 2001 CATCAAGCAA AACCTGTTTT TCGCCTTCTT CTACAATATT TTGGGCATTC 2051 CTCTCGCCGC GCTTGGCTTT TTAAATCCCG TCATCGCTGG CGCGGCAATG 2101 GCGGCAAGCT CGGTTTCCGT GTTGAGCAAT GCCTTGCGCC TGAAACGGGT 2151 AAAAATCGAT TAG This corresponds to the amino acid sequence <SEQ ID 1830; ORF 589>: m589.pep.. 1 MQQKIRFQIE GMTCQACASR IEKVLNKKDF VESAGVNFAS EEAQWFDDS 51 KTSVADIAKI IEKTGYGAKE KTEDTLPQPE AEHHIGWRLW LLFTINVPFL 101 IGMAGMMIGR HDWMIPPLWQ FALASWQLW LAIPFYKSAW ASIKGGLANM 151 DVLVTIGTVS IYLYSVYMLF FSPHAAYGMA HVYFEVGVMV IGFVSLGKFL 201 EHRTKKSSLN SLGLLLKLTP TQVNVQRNGE WKQLPIDQVQ IGDLIRANHG 251 ERIAADGIIE SGSGWADESH LTGESNPEEK KAGGKVLAGA LMTEGSWYR 301 ATQLGSQTQL GDMMNALSEA QGSKAPIARV ADKAAAVFVP AWGIALLTF 351 IVTWLIKGDW TVALMHAVAV LVIACPCALG LATPAAIMVG MGKAVKHGIW 401 FKDAAAMEEA AHVDAWLDK TGTLTEGSPQ VAAVYCVPDS GFDEDALYRI 451 AAAVEQNAAH PLARAIVSAA QARGLDIPAA QNAQTWGAG ITAEVEGVGL 501 VKAGKAEFAE LALPKFLDGV WDIASIVAVS VDNKPIGAFA LADALKADTA 551 EAIGRLKKHN IDVYIMSGDN QGTVEYVAKQ LGIAHAFGNM SPRDKAAEVQ 601 KLKAAGKTVA MVGDGINDAP ALAAANVSFA MKGGADVAEH TASATLMQHS 651 VNQLADALLV SQATLKNIKQ NLFFAFFYNI LGIPLAALGF LNPVIAGAAM 701 AASSVSVLSN ALRLKRVKID * Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m589 / g589 94.2% identity in 725 aa overlap 10 20 30 40 50 60 m589.pep M QQ KIRF Q IEGMTC Q ACASRIEKVLΝKKDFVESAGVΝFASEEAQWFDDSKTSVADIAKI I M I II II II M II I I II I I I I II I I I M I I I I I I I I I I I I M I I : I I lllllllll! g589 MQQKIRFQIEAMTCQACASRIEKVLNKKDFVESAGVNFASEEAQVTFDGSKTSVADIAKI 10 20 30 40 50 60 70 80 90 100 1 110 m589.pep IEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLFTINVPFLIGMAGMMIG RHDWMI I II I II II I I I II II I II II II II I II I II II : I I I : II I II M I I I : llllll g589 IEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLLTINIPFLIGMVGMMLKGLNWTRHDWMI 70 80 90 100 110 120 120 130 140 150 160 170 m589.pep PPLWQFALASVVQLWLAIPFYKSAWASIKGGLANMDVLVTIGTVSIYLYSVYMLFFSPHA I I : II I : II I : II II II II II I I II I II II II II II II II II II II II II II II II I II g589 PPVWQFVLASIVQLWLAIPFYKSAWASIKGGLANMDVLVTIGTVSIYLYSVYMLFFSSHA 130 140 150 160 170 180 180 190 200 210 220 230 m589. pep AYGMAHVYFEVGVMVIGFVSLGKFLEHRTKKSSLNSLGLLLKLTPTQVNVQRNGEWKQLP MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g589 AHGMAHVYFEAGVMVIGFVSLGKFLEHRTKKSSLNSLGLLLKLTPTQVNVQRNGEWKQLP 190 200 210 220 230 240 240 250 260 270 280 290 m589.pep IDQVQIGDLIRANHGERIAADGIIESGSGWADESHLTGESNPEEKKAGGKVLAGALMTEG II II I I II II I : I II II II II II II I II II II I I I II I I I I II I I II I II I I I I I I I I I I g589 IDQVQIGDLIRTNHGERIAADGI IESGSGWADESHLTGESNPEEKKAGGKVLAGALMTEG 250 260 270 280 290 300 300 310 320 330 340 350 m589 . pep SWYRATQLGSQTQLGDMMNALSEAQGSKAPIARVADKAAAVFVPAVVGIALLTFIVTWL I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I : I I g589 SWYRAAQLGSQTLLGDMMNALSEAQGSKAPIARVADKAAAVFVPTWGIALLTFIVAWL 310 320 330 340 350 360 360 370 380 390 400 410 m589. pep IKGDWTVALMHAVAVLVIACPCALGLATPAAIMVGMGKAVKHGIWFKDAAAMEEAAHVDA MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g589 IKGDWTVALMHAVAVLVIACPCALGLATPAAIMVGMGKAVKHGIWFKDAAAMEEAAHVDA 370 380 390 400 410 420 420 430 440 450 460 470 m589. pep WLDKTGTLTEGSPQVAAVYCVPDSGFDEDALYRIAAAVEQNAAHPLARAIVSAAQARGL II II II II II II II II I II II II II II II II II I II II I II II II II II I I g589 WLDKTGTLTEGRPQVAAVYYVPDSGFDEDALYRIAAAVEQNAAHPLARAIVSAAQARGL 430 440 450 460 470 480 480 490 500 510 520 530 m589. pep DIPAAQNAQTWGAGITAEVEGVGLVKAGKAEFAELALPKFLDGVWDIASIVAVSVDNKP : II II II II II II I II II II I I II II I : II II II II : II II II II : I I I I : I II : : I I g589 EIPAAQNAQTWGAGITAEVEGVGLVKSGKAEFAELTLPKFSDGVWEIASAVTVSVNGKP 490 500 510 520 530 540 540 550 560 570 580 590 m589. pep IGAFALADALKADTAEAIGRLKKHNIDVYIMSGDNQGTVEYVAKQLGIAHAFGNMSPRDK II II II : I II I II I II II II I II II II II II II II I : II II II II I II II II I I II I II g589 IGAFALΞDALKADTAEAIGRLKKHNIDVYIMSGDNQSTVEYVAKQLGIAHAFGNMSPCDK 550 560 570 580 590 600 600 610 620 630 640 650 m589. pep AAEVQKLKAAGKTVAMVGDGINDAPALAAANVSFAMKGGADVAEHTASATLMQHSVNQLA I II II II II II II II II I II I II II I II II II II II II II II II I II I II II II II I II I g589 AAEVQKLKAAGKTVAMVGDGINDAPALAAANVSFAMKGGADVAEHTASATLMQHSVNQLA 610 620 630 640 650 660 660 670 680 690 700 710 m589. pep DALLVSQATLKNIKQNLFFAFFYNILGIPLAALGFLNPVIAGAAMAASSVSVLSNALRLK II II : II II I : I II II II II I II II I II II II II I I II II II II I II I II II I : I II II I g589 DALLISQATLENIKQNLFFAFFYNILGIPLAALGFLNPVIAGAAMAASSVSVLGNALRLK 670 680 690 700 710 720 720 m589.pep RVKIDX II II I g589 WVKIDX The following partial DNA sequence was identified in N meningitidis <SEQ ID 1831>: a589. seq 1 ATGCAACAAA AAGTCCGTTT CCAAATCGAA GGCATGACCT GCCAGGCATG 51 TGCTTCGCGC ATTGAAAAAG TGTTGAACAA AAAAGATTTT GTCGAATCGG 101 CGGGGGTAAA CTTCGCCAGC GAAGAGGCTC AGGTAGTGTT TGACGACAGC 151 AAAACCTCAG TAGCCGACAT TGCCAAAATC ATTGAGAAAA CCGGTTACGG 201 CGCGAAGGAA AAAACGGAAG ATACATTGCC GCAACCCGAA GCAGAACACC 251 ATATCGGCTG GAGGTTGTGG CTTTTGCTGG CCATCAATAT CCCGTTCCTT 301 ATCGGTATGG TAGGGATGAT GCTAAAAGGG CTGAATTGGA CACGGCATGA 351 TTGGATGTTG TCGCCCTTGT TGCAGTTTGC ATTGGCGAGT GTGGTGCAGC 401 TTTGGCTGGC GGTGCCATTT TACAAAAGCG CGTGGGCGAG CATTAAAGGC 451 GGGCTGGCGA ATATGGACGT ACTCGTTACC ATCGGCACGG TCTCGATTTA 501 CCTGTATTCC GTCTATATGC TGTTTTTCAG CCCGCACGCG GCGTACGGTA 551 TGGCGCATGT GTATTTTGAA GTAGGCATAA TGGTGATTGG TTTTGTGTCA 601 CTGGGTAAAT TTTTGGAACA CCGCACCAAA AAATCCAGCC TGAACAGCTT 651 GGGCTTGCTG CTCAAACTCA CGCCAACCCA AGTCAACGTG CAACGCGATG 701 GCGAATGGCG GCAGCTACCC ATCGACCAAG TGCAAATCGG CGACCTAATC 751 CGCGCCAATC ACGGCGAACG CATTGCCGCC GACGGCATCA TAGAAAGCGG 801 CAGCGGCTGG GCGGACGAAA GCCATCTTAC CGGCGAATCC AATCCCGAAG 851 AGAAAAAGGC AGGCGGCAAA GTATTGGCGG GCGCGCTGAT GACTGAAGGC 901 AGCGTGGTGT ACCGCGCCGC GCAGCTCGGC AGCCAAACCC TGCTCGGCGA 951 CATGATGAAC GCGCTCTCCG AAGCGCAAGG CAGTAAAGCA CCGATTGCGC 1001 GTGTGGCGGA CAAGGCGGCG GCGGTATTCG TGCCTGCCGT TGTGGGCATC 1051 GCACTTTTGA CTTTTATCGC TACTTGGCTG ATTAAGGGCG ATTGGACGCT 1101 CGCATTGATG CACGCCGTCG CCGTTTTGGT GATTGCCTGC CCGTGTGCAC 1151 TCGGTTTGGC AACCCCTGCT GCGATTATGG TCGGTATGGG CAAAGCGGTT 1201 AAACACGGTA TTTGGTTTAA AGACGCGGCA GCAATGGAAG AAGCCGCCCA 1251 CGTTGATGCC GTCGTGCTGG ACAAAACCGG CACGCTGACC GAAGGCAAGC 1301 CGCAGGTTGC CGCCGTTTAT TGTGTTCCCG ACAGCGGCTT TGACGAAGAC 1351 GCTTTGTACC GCATCGCCGC CGCCGTCGAA CAAAACGCCG CCCATCCGCT 1401 CGCCCGTGCC ATCGTCTCCG CCGCCCAGGC GCGCGGTTTG GAGATTCCCA 1451 CCGCACAAAA TGCCCAAACC ATTGTCGGCG CGGGCATTAC CGCCGAAGTA 1501 AAAGGCGCGG GTTTGGTAAA AGCAGGCAAA GCCGAATTTG CCGAACTGAC 1551 CTTGCCGAAG TTTTCAGACG GCGTTTGGGA AATCGCCAGT GTGGTTGCCG 1601 TATCTGTAAA CGGCAAACCT ATCGGCGCAT TCGCACTCGC CGACGCGTTG 1651 AAAGCCGATA CCGCCGAAGC CATAGGCCGT CTGAAAAAAC ACAATATCGA 1701 TGTCTATATT ATGAGCGGCG ATAACCAAGG CACGGTCGAG TACGTCGCCA 1751 AACAACTGGG CATCGCACAC GCCTTCGGTA ATATGAGTCC GCGCGACAAA 1801 GCCGCCGAAG TGCAGAAACT CAAAGCCGCC GGCAAAACCG TGGCGATGGT 1851 CGGCGACGGC ATCAACGACG CGCCCGCGCT CGCCGCCGCC AACGTCAGCT 1901 TCGCCATGAA AGGCGGTGCA GACGTTGCCG AACACACCGC ATCCGCCACA 1951 CTGATGCAGC ATTCGGTCAA CCAGCTCGCC GATGCGCTAT CGGTATCGCG 2001 AGCGACGTTG AAAAACATCA AGCAAAACCT GTTTTTCGCC TTCTTCTACA 2051 ATATTTTGGG CATTCCGCTC GCCGCGCTCG GCTTTTTAAA CCCCGTCATC 2101 GCAGGCGCGG CAATGGCGGC AAGCTCGGTT TCCGTGTTGA GCAACGCCTT 2151 GCGCCTGAAA CGGGTAAAAA TCGATTGA This corresponds to the amino acid sequence <SEQ ID 1832; ORF 589. a>: a589.pep 1 MQQKVRFQIE GMTCQACASR IEKVLNKKDF VESAGVNFAS EEAQWFDDS 51 KTSVADIAKI IEKTGYGAKE KTEDTLPQPE AEHHIGWRLW LLLAINIPFL 101 IGMVGMMLKG LNWTRHDWML SPLLQFALAS WQLWLAVPF YKSAWASIKG 151 GLANMDVLVT IGTVSIYLYS VYMLFFSPHA AYGMAHVYFE VGIMVIGFVS 201 LGKFLEHRTK KSSLNSLGLL LKLTPTQVNV QRDGEWRQLP IDQVQIGDLI 251 RANHGERIAA DGIIESGSGW ADESHLTGES NPEEKKAGGK VLAGALMTEG 301 SWYRAAQLG SQTLLGDMMN ALSEAQGSKA PIARVADKAA AVFVPAWGI 351 ALLTFIATWL IKGDWTLALM HAVAVLVIAC PCALGLATPA AIMVGMGKAV 401 KHGIWFKDAA AMEEAAHVDA WLDKTGTLT EGKPQVAAVY CVPDSGFDED 451 ALYRIAAAVE QNAAHPLARA IVSAAQARGL EIPTAQNAQT IVGAGITAEV 501 KGAGLVKAGK AEFAELTLPK FSDGVWEIAS WAVSVNGKP IGAFALADAL 551 KADTAEAIGR LKKHNIDVYI MSGDNQGTVE YVAKQLGIAH AFGNMSPRDK 601 AAEVQKLKAA GKTVAMVGDG INDAPALAAA NVSFAMKGGA DVAEHTASAT 651 LMQHSVNQLA DALSVSRATL KNIKQNLFFA FFYNILGIPL AALGFLNPVI 701 AGAAMAASSV SVLSNALRLK RVKID* m589/a589 94.9% identity in 725 aa overlap 10 20 30 40 50 60 m589.pep MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQVVFDDSKTSVADIAKI MM MMMMMMMMMMMMMMMMMMMMMMMMMMM a589 MQQKVRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQWFDDSKTSVADIAKI 10 20 30 40 50 60 70 80 90 100 1 110 m589. pep IEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLFTINVPFLIGMAGMMIG RHDWMI II II II II II II I II II I II II I I II I II I IM: II : II II II : II I: Mill: a589 IEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLLAINIPFLIGMVGMMLKGLNWTRHDWML 70 80 90 100 110 120 120 130 140 150 160 170 m589.pep PPLWQFALASVVQLWLAIPFYKSAWASIKGGLANMDVLVTIGTVSIYLYSVYMLFFSPHA II I II II II II II II : II II I II II I II I II I I II II I I II II I II I I II I I I I II II a589 SPLLQFALASVVQLWLAVPFYKSAWASIKGGLANMDVLVTIGTVSIYLYSVYMLFFSPHA 130 140 150 160 170 180 180 190 200 210 220 230 m589. pep AYGMAHVYFEVGVMVIGFVSLGKFLEHRTKKSSLNSLGLLLKLTPTQVNVQRNGEWKQLP II II II II II II : II II II I II I II II II M M M I I M M I I II I II II II : II I: II I a589 AYGMAHVYFEVGIMVIGFVSLGKFLEHRTKKSSLNSLGLLLKLTPTQVNVQRDGEWRQLP 190 200 210 220 230 240 240 250 260 270 280 290 m589.pep IDQVQIGDLIRANHGERIAADGIIESGSGWADESHLTGESNPEEKKAGGKVLAGALMTEG II II II II I II II II I II II II I I I I II II II I I I I I II II II II II II I II I I I I I I I I a589 IDQVQIGDLIRANHGERIAADGIIESGSGWADESHLTGESNPEEKKAGGKVLAGALMTEG 250 260 270 280 290 300 300 310 320 330 340 350 m589. pep SWYRATQLGSQTQLGDMMNALSEAQGSKAPIARVADKAAAVFVPAWGIALLTFIVTWL II II I I : II II II II II II II II II I I II II II II II II II I II I I II I I I I I I I : I I I a589 SWYRAAQLGSQTLLGDMMNALSEAQGSKAPIARVADKAAAVFVPAWGIALLTFIATWL 310 320 330 340 350 360 360 370 380 390 400 410 m589. pep IKGDWTVALMHAVAVLVIACPCALGLATPAAIMVGMGKAVKHGIWFKDAAAMEEAAHVDA MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a589 IKGDWTLALMHAVAVLVIACPCALGLATPAAIMVGMGKAVKHGIWFKDAAAMEEAAHVDA 370 380 390 400 410 420 420 430 440 450 460 470 m589. pep WLDKTGTLTEGSPQVAAVYCVPDSGFDEDALYRIAAAVEQNAAHPLARAIVSAAQARGL MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a589 WLDKTGTLTEGKPQVAAVYCVPDSGFDEDALYRIAAAVEQNAAHPLARAIVSAAQARGL 430 440 450 460 470 480 480 490 500 510 520 530 m589. pep DIPAAQNAQTVVGAGITAEVEGVGLVKAGKAEFAELALPKFLDGVWDIASIVAVSVDNKP : I I M II I II : I II II II II M M I II II II I II I I : II II II II : I I I : II I II : : I I a589 ElPTAQNAQTIVGAGITAEVKGAGLVKAGKAEFAELTLPKFSDGVWEIASWAVSVNGKP 490 500 510 520 530 540 540 550 560 570 580 590 m589. pep IGAFALADALKADTAEAIGRLKKHNIDVYIMSGDNQGTVEYVAKQLGIAHAFGNMSPRDK II II II II II II I II I II II II II I I I I II II II I II II II II II II I II I II II I II II a589 IGAFALADALKADTAEAIGRLKKHNIDVYIMSGDNQGTVEYVAKQLGIAHAFGNMSPRDK 550 560 570 580 590 600 600 610 620 630 640 650 m589. pep AAEVQKLKAAGKTVAMVGDGINDAPALAAANVSFAMKGGADVAEHTASATLMQHSVNQLA II II II II II II II II II II I II II II II I II I II II II II II II II II II II II II II I a589 AAEVQKLKAAGKTVAMVGDGINDAPALAAANVSFAMKGGADVAEHTASATLMQHSVNQLA 610 620 630 640 650 660 660 670 680 690 700 710 m589. pep DALLVSQATLKNIKQNLFFAFFYNILGIPLAALGFLNPVIAGAAMAASSVSVLSNALRLK III II : I II I II II II II II II I II II I II II I I I II II I II II II I I I II I II I I II I a589 DALSVSRATLKNIKQNLFFAFFYNILGIPLAALGFLNPVIAGAAMAASSVSVLSNALRLK 670 680 690 700 710 720 720 m589.pep RVKIDX a589 RVKIDX The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1833>: g590.seq.. 1 atgaaaaaac ctttgatttc agttgcggca gtattgctcg gcgttgcttt 51 gggtacacct tattatttgg gtgtcaaagc agaagaaagt ctgacgcagc 101 agcaaaaaat attgcagaaa acgggctttt tgaccgtcga atcgcaccag 151 tatgatcgag gctggtttac ctctacggaa acgacggtca tccgtctgaa 201 acccgagttg ctgcataatg cgcagaaata cctgccggat aacttgaaaa 251 tagtgttgga acagccggtt acgctggtaa accatatcac gcacggccct 301 ttcgccggcg gattcggcac gcaggcgcac attgaaaccg agttcaaata 351 cgcgcctgaa acggaaaaag ttttggaacg cttttttggg aaacaagttc 401 cggtttccct tgccaatacc gtttatttca acggcagcgg taaaatggaa 451 gtcagtgttc ccgctttcga ttatgaagaa ctgtcgggca tcaggctgca 501 ctgggaaggc ctgacggggg aaacggttta tcaaaaaggt ttcaaaagct 551 accgcaacag ctatgatgcg cccttgttca aaatcaagct ggcagacaaa 601 ggcgatgccg cgtttgaaaa agcgcatttc gattcggaaa cttcagacgg 651 catcaatccg cttgctttgg gcagcagcaa tctgactttg gaaaaatttt 701 cgctcgaatg gaaagagggt gtcgattaca acgtcaaatt gaacgaactg 751 gtcaacctcg ttaccgattt gcagatcggc gcgtttatca atcccaacgg 801 cagcatcgca ccttccaaaa tcgaagtcgg caagctggct ttttcaacca 851 agaccgggga atcgggcgcg tttatcgaca gcgaagggcg gttccgtttc 901 gatacgttgg tgtacggcga tgaaaaatac ggcccgctgg acatccatat 951 cgctgccgaa cacctcgatg cttctgcctt aaccgtattg aaacgcaagt 1001 ttgcacaaat ttctgccaaa aaaatgactg aggaacaaat ccgcaatgat 1051 ttgattgcgg cagtcaaagg cgatgcttcc ggattattta cccatgaccc 1101 ggtactaaat atcaaaattt tccgtttcac cctgcctcag ggaaaaattg 1151 atgtgggcgg aaaaatcatg tttaaaggca tgaagaagga agatttgaac 1201 caattgggac tgatgttaaa gaaaaccgag gcaaacatca gaatgagtat 1251 tcctcaaaaa atgttggaag atttggcggt aagtcaggct ggaaatattt 1301 tcagtgtaaa tgccgaagat gaggcggaag ccagagcaag cattgccgat 1351 attaatgaaa cattgcgcct gatggtggac agtacggtcc aaagtatggc 1401 aagggaaaaa tatcttactt tagacggtaa tcagattgat acggtcattt 1451 cccttaaaaa caacgccctg aagttaaacg ggaaaacgct gcaaaatgaa 1501 cccgatcctg attttgacga gggagatatg gtttccggcc agccgcatta 1551 a This corresponds to the amino acid sequence <SEQ ID 1834; ORF 590.ng>: g590.pep.. 1 MKKPLISVAA VLLGVALGTP YYLGVKAEES LTQQQKILQK TGFLTVESHQ 51 YDRGWFTSTE TTVIRLKPEL LHNAQKYLPD NLKIVLEQPV TLVNHITHGP 101 FAGGFGTQAH IETEFKYAPE TEKVLERFFG KQVPVSLANT VYFNGSGKME 151 VSVPAFDYEE LSGIRLHWEG LTGETVYQKG FKSYRNSYDA PLFKIKLADK 201 GDAAFEKAHF DSETSDGINP LALGSSNLTL EKFSLEWKEG VDYNVKLNEL 251 VNLVTDLQIG AFINPNGSIA PSKIEVGKLA FSTKTGESGA FIDSEGRFRF 301 DTLVYGDEKY GPLDIHIAAE HLDAΞALTVL KRKFAQISAK KMTEEQIRND 351 LIAAVKGDAS GLFTHDPVLN IKIFRFTLPQ GKIDVGGKIM FKGMKKEDLN 401 QLGLMLKKTE ANIRMSIPQK MLEDLAVSQA GNIFSVNAED EAEARASIAD 451 INETLRLMVD STVQSMAREK YLTLDGNQID TVISLKNNAL KLNGKTLQNE 501 PDPDFDEGDM VSGQPH* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1835>: m590.seq (partial) 1 .. TGGTTTACCT CTATGGAAAC GACGGTCATC CGTCTGAAAC CCGAGTTGCT 51 GAATAATGCC CGAAAATACC TGCCGGATAA CCTGAAAACA GTGTTGGAAC 101 AGCCGGTTAC GCTGGTTAAC CATATCACGC ACGGCCCTTT CGCCGGCGGA 151 TTCGGCACGC AGGCGTACAT TGAAACCGAG TTCAAATACG CGCCTGAAAC 201 GGAAAAAGTT CTGGAACGCT TTTTTGGAAA ACAAGTCCCG GCTTCCCTTG 251 CCAATACCGT TTATTTTAAC GGCAGCGGTA AAATGGAAGT CAGTGTTCCC 301 GCCTTCGATT ATGAAGAGCT GTCGGGCATc AG.CTGCACT GGGAA GCCT 351 GACGGGAGAA ACGGTTTATC AAAAAGGTTT CAAAAGCTAC CGGAACGGCT 401 ATGATGCCCC CTTGTTTAAA ATCAAGCTGG CAGACAAAGG CGATGCCGCG 451 TTTGAAAAAG TGCATTTCGA TTCGGAAACT TCAGACGGCA TCAATCCGCT 501 TGCTTTGGGC AGCAGCAATC TGACCTTGGA AAAATTCTCC CTAGAATGGA 551 AAGAGGGTGT CGATTACAAC GTCAAGTTAA ACGAACTGGT CAATCTTGTT 601 ACCGATTTGC AGATTGGCGC GTTTATCAAT CCCAACGGCA GCATCGCACC 651 TTCCAAAATC GAAGTCGGCA AACTGGCTTT TTCAACCAAG ACCGGGGAAT 701 CAGGCGCGTT TATCAACAGT GAAGGGCAGT TCCGTTTCGA TACACTGGTG 751 TACGGCGATG AAAAATACGG CCCGCTGGAC ATCCATATCG CTGCCGAACA 801 CCTCGATGCT TCTGCCTTAA CCGTATTGAA ACGCAAGTTT GCACAAATTT 851 CCGCCAAAAA AATGACCGAG GAACAAATCC GCAATGATTT GATTGCCGCC 901 GTCAAAGGAG AGGCTTCCGG ACTGTTCACC AACAATCCCG TATTGGACAT 951 TAAAACTTTC CGATTCACGC TGCCATCGGG AAAAATCGAT GTGGGCGGAA 1001 AAATCATGTT TAAAGACATG AAGAAGGAAG ATTTGAATCA ATTGGGTTTG 1051 ATGCTGAAGA AAACCGAAGC CGACATCAGA ATGAGTATTC CCCAAAAAAT 1101 GCTGGAAGAC TTGGCGGTCA GTCAAGCAGG CAATATTTTC AGCGTCAATG 1151 CCGAAGATGA GGCGGAAGGC AGGGCAAGTC TTGACGACAT CAACGAGACC 1201 TTGCGCCTGA TGGTGGACAG TACGGTTCAG AGTATGGCAA GGGAAAAATA 1251 TCTGACTTTG AACGGCGACC AGATTGATAC TGCCATTTCT CTGAAAAACA 1301 ATCAGTTGAA ATTGAACGGT AAAACGTTGC AAAACGAACC GGAGCCGGAT 1351 TTTGATGAAG GCGGTATGGT TTCAGAGCCG CAGCAGTAA This corresponds to the amino acid sequence <SEQ ID 1836; ORF 590>: m590.pep.. (partial) 1 ..WFTSMETTVI RLKPELLNNA RKYLPDNLKT VLEQPVTLVN HITHGPFAGG 51 FGTQAYIETE FKYAPETEKV LERFFGKQVP ASLANTVYFN GΞGKMEVSVP 101 AFDYEELSGI XLHWEXLTGE TVYQKGFKSY RNGYDAPLFK IKLADKGDAA 151 FEKVHFDΞET ΞDGINPLALG SSNLTLEKFS LEWKEGVDYN VKLNELVNLV 201 TDLQIGAFIN PNGSIAPSKI EVGKLAFΞTK TGESGAFINS EGQFRFDTLV 251 YGDEKYGPLD IHIAAEHLDA SALTVLKRKF AQISAKKMTE EQIRNDLIAA 301 VKGEASGLFT NNPVLDIKTF RFTLPSGKID VGGKIMFKDM KKEDLNQLGL 351 MLKKTEADIR MSIPQKMLED LAVSQAGNIF SVNAEDEAEG RASLDDINET 401 LRLMVDΞTVQ SMAREKYLTL NGDQIDTAIS LKNNQLKLNG KTLQNEPEPD 451 FDEGGMVSEP QQ* m590 / g590 93.1% identity in 462 aa overlap 10 20 30 m590.pep WFTSMETTVIRLKPELLNNARKYLPDNLKT MM g590 VKAEESLTQQQKILQKTGFLTVESHQYDRGWFTSTETTVIRLKPELLHNAQKYLPDNLKI 30 40 50 60 70 80 40 50 60 70 80 90 m590.pep VLEQPVTLVNHITHGPFAGGFGTQAYIETEFKYAPETEKVLERFFGKQVPASLANTVYFN i 111 i 1111111111111111111 I !! 111111111111 ! 11111111 : 111111111 g590 VLEQPVTLVNHITHGPFAGGFGTQAHIETEFKYAPETEKVLERFFGKQVPVSLANTVYFN 90 100 110 120 130 140 100 110 120 130 140 150 m590.pep GSGKMEVSVPAFDYEELSGIXLHWEXLTGETVYQKGFKSYRNGYDAPLFKIKLADKGDAA 111111 II 111111111 M I llll MMMMMMMIMIMMMMIIMMM g590 GSGKMEVSVPAFDYEELSGIRLHWEGLTGETVYQKGFKSYRNSYDAPLFKIKLADKGDAA 150 160 170 180 190 200 160 170 180 190 200 210 m590.pep FEKVHFDSETSDGINPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFIN MhllMMMMMIIMMMIMMMMMMMMIIIMMMMMMMM g590 FEKAHFDSETSDGINPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFIN 210 220 230 240 250 260 220 230 240 250 260 270 m590.pep PNGSIAPSKIEVGKLAFSTKTGESGAFINSEGQFRFDTLVYGDEKYGPLDIHIAAEHLDA I : M M M IIMMMMM I : MM IMMi ιM g590 PNGSIAPSKIEVGKLAFSTKTGESGAFIDSEGRFRFDTLVYGDEKYGPLDIHIAAEHLDA 270 280 290 300 310 320 280 290 300 310 320 330 m590.pep SALTVLKRKFAQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTLPSGKID I M 11111 ! i 1111 ! 111111111111 i 111 i ! H 11111 = : 111 : i I IIIMMMM g590 SALTVLKRKFAQISAKKMTEEQIRNDLIAAVKGDASGLFTHDPVLNIKIFRFTLPQGKID 330 340 350 360 370 380 340 350 360 370 380 390 m590.pep VGGKIMFKDMKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEG MIIIMMIMMMIMMIMMMMIMMMIMMMMMIh g590 VGGKIMFKGMKKEDLNQLGLMLKKTEANIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEA 390 400 410 420 430 440 400 410 420 430 440 450 m590.pep RASLDDINETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPD M 1111 II 111111111111111 Ml l II I = 111111 MIMIMMIhll g590 RASIADINETLRLMVDSTVQSMAREKYLTLDGNQIDTVISLKNNALKLNGKTLQNEPDPD 450 460 470 480 490 500 460 m590.pep FDEGGMVΞ-EPQQX llll III g590 FDEGDMVSGQPHX 510 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1837>: a590. seq 1 ATGAAAAAAC CTTTGATTTC GGTTGCGGCA GCATTGCTCG GCGTTGCTTT 51 GGGCACGCCT TATTATTTGG GTGTCAAAGC CGAAGAAAGC TTGACGCAGC 101 AGCAAAAAAT ATTGCAGGAA GCGGGCTTCT TGACCGTCGA ATCGCACCAA 151 TATGAGCGCG GCTGGTTTAC CTCTACGGAA ACGACGGTCA TCCGCTTGAA 201 ACCCGAGTTG CTGCATAATG CGCAGAAATA CCTGCCGGAT AACCTGAAAA 251 CAGTGTTGGA ACAGCCGGTT ACGCTGGTAA ACCATATCAC GCACGGTCCT 301 TTTGCCGGCG GATTCGGCAC GCAGGCGTAC ATTGAAACCG AGTTCAAATA 351 CGCGCCTGAA ACGGAAAAAG TTCTGGAACG CTTTTTTGGA AAACAAGTCC 401 CGGTTTCCCT TGCCAATACC GTTTATTTTA ACGGCAGCGG TAAAATGGAA 451 GTCAGTGTTC CCGCCTTCGA TTATGAAGAG CTGTCGGGCA TCAGGCTGCA 501 CTGGGAAGGC CTGACGGGAG AAACGGTTTA TCAAAAAGGT TTCAAAAGCT 551 ACCGGAACGG CTATGATGCC CCCTTGTTTA AAATCAAGCT GGCAGACAAA 601 GGCGATGCCG CGTTTGAAAA AGTGCATTTC GATTCGGAAA CTTCAGACGG 651 CATCAACCCG CTTGCTTTGG GCAGCAGCAA TCTGACCTTG GAAAAATTTT 701 CCTTAGAATG GAAAGAGGGT GTCGATTACA ACGTCAAGTT AAACGAACTG 751 GTCAATCTTG TTACCGATTT GCAGATTGGC GCGTTTATCA ATCCCAACGG 801 CAGCATCGCA CCTTCCAAAA TCGAAGTCGG CAAGCTGGCT TTTTCAACCA 851 AGACCGGGGA ATCGGGCGCG TTTATCGATA GCGAAGGGCA GTTCCGTTTT 901 GGCACGCTGG TTTACGGCGA TGAAAAATAC GGCCCTCTGG ACATCCATAT 951 CGCTGCCGAA CACCTCGATG CTTCTGCCTT AACCGTATTG AAACGCAAGT 1001 TTGCACGAAT TTCTGCCAAA AAAATGACTG AAGAACAAAT CCGCAATGAT 1051 TTGATTGCGG CAGTCAAAGG CGAGGCTTCC GGATTATTTA CCCATAACCC 1101 AGTATTGGAC ATTAAAACTT TCCGATTCAC GCTGCCATCG GGAAAAATCG 1151 ATGTGGGCGG AAAAATCATG TTTAAAGACA TGAAGAAGGA AGATTTGAAC 1201 CAATTGGGTT TGATGCTGAA GAAAACCGAA GCCGACATCA GAATGAGTAT 1251 TCCCCAAAAA ATGCTGGAAG ACTTGGCGGT CAGTCAAGCA GGCAATATTT 1301 TCAGCGTCAA TGCCGAAGAT GAGGCGGAAG GCAGGGCAAG TCTTGACGAC 1351 ATCAACGAGA CCTTGCGCCT GATGGTGGAC AGTACGGTTC AGAGTATGGC 1401 AAGGGAAAAA TATCTGACTT TGAACGGCGA CCAGATTGAT ACTGCCATTT 1451 CTCTGAAAAA CAATCAGTTG AAATTGAACG GTAAAACGTT GCAAAACGAA 1501 CCGGAGCCGG ATTTTGATGA AGGCGGTATG GTTTCAGAGC CGCAGCAGTA 1551 A This corresponds to the amino acid sequence <SEQ ID 1838; ORF 590. a>: a590.pep 1 MKKPLISVAA ALLGVALGTP YYLGVKAEES LTQQQKILQE AGFLTVESHQ 51 YERGWFTSTE TTVIRLKPEL LHΝAQKYLPD ΝLKTVLEQPV TLVΝHITHGP 101 FAGGFGTQAY IETEFKYAPE TEKVLERFFG KQVPVSLAΝT VYFΝGSGKME 151 VSVPAFDYEE LSGIRLHWEG LTGETVYQKG FKSYRΝGYDA PLFKIKLADK 201 GDAAFEKVHF DSETSDGIΝP LALGSSΝLTL EKFSLEWKEG VDYΝVKLΝEL 251 VΝLVTDLQIG AFIΝPΝGSIA PSKIEVGKLA FSTKTGESGA FIDSEGQFRF 301 GTLVYGDEKY GPLDIHIAAE HLDASALTVL KRKFARISAK KMTEEQIRΝD 351 LIAAVKGEAS GLFTHΝPVLD IKTFRFTLPS GKIDVGGKIM FKDMKKEDLΝ 401 QLGLMLKKTE ADIRMSIPQK MLEDLAVSQA GΝIFSVΝAED EAEGRASLDD 451 IΝETLRLMVD STVQSMAREK YLTLΝGDQID TAISLKΝΝQL KLΝGKTLQΝE 501 PEPDFDEGGM VSEPQQ* m590/a590 97.8% identity in 462 aa overlap 10 20 30 m590.pep WFTSMETTVIRLKPELLΝΝARKYLPDΝLKT MM II II I II II II I : II : II II II I I I a590 VKAEESLTQQQKILQEAGFLTVESHQYERGWFTSTETTVIRLKPELLHΝAQKYLPDΝLKT 30 40 50 60 70 80 40 50 60 70 80 90 m590. pep VLEQPVTLVΝHITHGPFAGGFGTQAYIETEFKYAPETEKVLERFFGKQVPASLAΝTVYFΝ II II I II II II II II II II II II II I II II I I I I I II I II I I I I II M I I: M I I M I I I a590 VLEQPVTLVΝHITHGPFAGGFGTQAYIETEFKYAPETEKVLERFFGKQVPVSLAΝTVYFΝ 90 100 110 120 130 140 100 110 120 130 140 150 m590. pep GSGKMEVSVPAFDYEELSGIXLHWEXLTGETVYQKGFKSYRΝGYDAPLFKIKLADKGDAA II II I I I I I II II II II II I llll II II I I II II II I II II I II I I II II I I I II I II a590 GSGKMEVSVPAFDYEELSGIRLHWEGLTGETVYQKGFKSYRNGYDAPLFKIKLADKGDAA 150 160 170 180 190 200 160 170 180 190 200 210 m590.pep FEKVHFDSETSDGINPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFIN II II II I II II I II II II I II II I II II II II I II II II II II II II II I I II I II I II I a590 FEKVHFDSETSDGINPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFIN 210 220 230 240 250 260 220 230 240 250 260 270 m590.pep PNGSIAPSKIEVGKLAFSTKTGESGAFINSEGQFRFDTLVYGDEKYGPLDIHIAAEHLDA II II II II II II I II II II II I II II II: II II I I I II II II I II II II I I I I I II I I I a590 PNGSIAPSKIEVGKLAFSTKTGESGAFIDSEGQFRFGTLVYGDEKYGPLDIHIAAEHLDA 270 280 290 300 310 320 280 290 300 310 320 330 m590. pep SALTVLKRKFAQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTLPSGKID II II II I II II : II II II II II I II I I II II I I II II II I : II II II I I I I I I II I I II I a590 SALTVLKRKFARISAKKMTEEQIRNDLIAAVKGEASGLFTHNPVLDIKTFRFTLPSGKID 330 340 350 360 370 380 340 350 360 370 380 390 m590. pep VGGKIMFKDMKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEG II II II I II I II II II II II II I II I II II II I II II I II I II II I I II II I I I II M I I a590 VGGKIMFKDMKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEG 390 400 410 420 430 440 400 410 420 430 440 450 m590. pep RASLDDINETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPD II II II I II I II II II I II II II II II II II I I II II II II II II I II II I I I I I II I I I a590 RASLDDINETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPD 450 460 470 480 490 500 460 m590.pep FDEGGMVSEPQQX a590 FDEGGMVSEPQQX 510 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1839>: m590-l.seq 1 ATGAAAAAAC CTTTGATTTC GGTTGCGGCA GCATTGCTCG GCGTTGCTTT 51 GGGCACGCCT TATTATTTGG GTGTCAAAGC CGAAGAAAGC TTGACGCAGC 101 AGCAAAAAAT ATTGCAGGAA ACGGGCTTCT TGACCGTCGA ATCGCACCAA 151 TATGAGCGCG GCTGGTTTAC CTCTATGGAA ACGACGGTCA TCCGTCTGAA 201 ACCCGAGTTG CTGAATAATG CCCGAAAATA CCTGCCGGAT AACCTGAAAA 251 CAGTGTTGGA ACAGCCGGTT ACGCTGGTTA ACCATATCAC GCACGGCCCT 301 TTCGCCGGCG GATTCGGCAC GCAGGCGTAC ATTGAAACCG AGTTCAAATA 351 CGCGCCTGAA ACGGAAAAAG TTCTGGAACG CTTTTTTGGA AAACAAGTCC 401 CGGCTTCCCT TGCCAATACC GTTTATTTTA ACGGCAGCGG TAAAATGGAA 451 GTCAGTGTTC CCGCCTTCGA TTATGAAGAG CTGTCGGGCA TCAGGCTGCA 501 CTGGGAAGGC CTGACGGGAG AAACGGTTTA TCAAAAAGGT TTCAAAAGCT 551 ACCGGAACGG CTATGATGCC CCCTTGTTTA AAATCAAGCT GGCAGACAAA 601 GGCGATGCCG CGTTTGAAAA AGTGCATTTC GATTCGGAAA CTTCAGACGG 651 CATCAATCCG CTTGCTTTGG GCAGCAGCAA TCTGACCTTG GAAAAATTCT 701 CCCTAGAATG GAAAGAGGGT GTCGATTACA ACGTCAAGTT AAACGAACTG 751 GTCAATCTTG TTACCGATTT GCAGATTGGC GCGTTTATCA ATCCCAACGG 801 CAGCATCGCA CCTTCCAAAA TCGAAGTCGG CAAACTGGCT TTTTCAACCA 851 AGACCGGGGA ATCAGGCGCG TTTATCAACA GTGAAGGGCA GTTCCGTTTC 901 GATACACTGG TGTACGGCGA TGAAAAATAC GGCCCGCTGG ACATCCATAT 951 CGCTGCCGAA CACCTCGATG CTTCTGCCTT AACCGTATTG AAACGCAAGT 1001 TTGCACAAAT TTCCGCCAAA AAAATGACCG AGGAACAAAT CCGCAATGAT 1051 TTGATTGCCG CCGTCAAAGG AGAGGCTTCC GGACTGTTCA CCAACAATCC 1101 CGTATTGGAC ATTAAAACTT TCCGATTCAC GCTGCCATCG GGAAAAATCG 1151 ATGTGGGCGG AAAAATCATG TTTAAAGACA TGAAGAAGGA AGATTTGAAT 1201 CAATTGGGTT TGATGCTGAA GAAAACCGAA GCCGACATCA GAATGAGTAT 1251 TCCCCAAAAA ATGCTGGAAG ACTTGGCGGT CAGTCAAGCA GGCAATATTT 1301 TCAGCGTCAA TGCCGAAGAT GAGGCGGAAG GCAGGGCAAG TCTTGACGAC 1351 ATCAACGAGA CCTTGCGCCT GATGGTGGAC AGTACGGTTC AGAGTATGGC 1401 AAGGGAAAAA TATCTGACTT TGAACGGCGA CCAGATTGAT ACTGCCATTT 1451 CTCTGAAAAA CAATCAGTTG AAATTGAACG GTAAAACGTT GCAAAACGAA 1501 CCGGAGCCGG ATTTTGATGA AGGCGGTATG GTTTCAGAGC CGCAGCAGTA 1551 A This corresponds to the amino acid sequence <SEQ ID 1840; ORF 590- 1>: m590-l.pep 1 MKKPLISVAA ALLGVALGTP _YYLGVKAEES LTQQQKILQE TGFLTVESHQ 51 YERGWFTSME TTVIRLKPEL LNNARKYLPD NLKTVLEQPV TLVNHITHGP 101 FAGGFGTQAY IETEFKYAPE TEKVLERFFG KQVPASLANT VYFNGSGKME 151 VSVPAFDYEE LSGIRLHWEG LTGETVYQKG FKSYRNGYDA PLFKIKLADK 201 GDAAFEKVHF DSETΞDGINP LALGSSNLTL EKFSLEWKEG VDYNVKLNEL 251 VNLVTDLQIG AFINPNGSIA PSKIEVGKLA FSTKTGESGA FINSEGQFRF 301 DTLVYGDEKY GPLDIHIAAE HLDASALTVL KRKFAQISAK KMTEEQIRND 351 LIAAVKGEAS GLFTNNPVLD IKTFRFTLPS GKIDVGGKIM FKDMKKEDLN 401 QLGLMLKKTE ADIRMSIPQK MLEDLAVSQA GNIFSVNAED EAEGRASLDD 451 INETLRLMVD STVQSMAREK YLTLNGDQID TAISLKNNQL KLNGKTLQNE 501 PEPDFDEGGM VSEPQQ* m590-l/g590 93.6% identity in 516 aa overlap 10 20 30 40 50 60 m590-l.pep MKKPLISVAAALLGVALGTPYYLGVKAEEΞLTQQQKILQETGFLTVEΞHQYERGWFTΞME I I I I I I I I I I: I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I: I I I I I I I g590 MKKPLISVAAVLLGVALGTPYYLGVKAEESLTQQQKILQKTGFLTVESHQYDRGWFTSTE 10 20 30 40 50 60 70 80 90 100 110 120 m590-l.pep TTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFAGGFGTQAYIETEFKYAPE I I I I I I I I I I I : I l I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I g590 TTVIRLKPELLHNAQKYLPDNLKIVLEQPVTLVNHITHGPFAGGFGTQAHIETEFKYAPE 70 80 90 100 110 120 130 140 150 160 170 180 m590-1.pep TEKVLERFFGKQVPAΞLANTVYFNGSGKMEVΞVPAFDYEELSGIRLHWEGLTGETVYQKG I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g590 TEKVLERFFGKQVPVSLANTVYFNGSGKMEVSVPAFDYEELSGIRLHWEGLTGETVYQKG 130 140 150 160 170 180 190 200 210 220 230 240 m590~l.pep FKΞYRNGYDAPLFKIKLADKGDAAFEKVHFDSETSDGINPLALGΞΞNLTLEKFSLEWKEG I I I I I I : I I I I I I I I I I I I I I I I I I I Ml I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I g590 FKSYRNΞYDAPLFKIKLADKGDAAFEKAHFDSETSDGINPLALGSΞNLTLEKFSLEWKEG 190 200 210 220 230 240 250 260 270 280 290 300 m590-1.pep VDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEVGKLAFSTKTGEΞGAFINSEGQFRF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I :| I I g590 VDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEVGKLAFSTKTGEΞGAFIDSEGRFRF 250 260 270 280 290 300 310 320 330 340 350 360 m590-l.pep DTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKFAQISAKKMTEEQIRNDLIAAVKGEAS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I g590 DTLVYGDEKYGPLDIHIAAEHLDAΞALTVLKRKFAQISAKKMTEEQIRNDLIAAVKGDAS 310 320 330 340 350 360 370 380 390 400 410 420 m590-l.pep GLFTNNPVLDIKTFRFTLPΞGKIDVGGKIMFKDMKKEDLNQLGLMLKKTEADIRMSIPQK I I I I ::| I I :| I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I g590 GLFTHDPVLNIKIFRFTLPQGKIDVGGKIMFKGMKKEDLNQLGLMLKKTEANIRMSIPQK 370 380 390 400 410 420 430 440 450 460 470 480 m590-1. pep MLEDLAVSQAGNIFSVNAEDEAEGRASLDDINETLRLMVDSTVQSMAREKYLTLNGDQID I I I I I I I I I I I I I I I I I I I I I I l I I : I I I I I I I I I I I I I I I I I I I I I I I I |:| : I I I g590 MLEDLAVSQAGNIFSVNAEDEAEARASIADINETLRLMVDSTVQΞMAREKYLTLDGNQID 430 440 450 460 470 480 490 500 510 m590-l .pep TAISLKNNQLKLNGKTLQNEPEPDFDEGGMVS-EPQQX I : I I I I I I I I I I I I I I I I I I M I I I I I III : I : g590 TVISLKNNALKLNGKTLQNEPDPDFDEGDMVΞGQPHX 490 500 510 a590/m590-l 98.3% identity in 516 aa overlap 10 20 30 40 50 60 a590.pep MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQEAGFLTVESHQYERGWFTSTE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I m590-l MKKPLISVAAALLGVALGTPYYLGVKAEEΞLTQQQKILQETGFLTVESHQYERGWFTΞME 10 20 30 40 50 60 70 80 90 100 110 120 a590.pep TTVIRLKPELLHNAQKYLPDNLKTVLEQPVTLVNHITHGPFAGGFGTQAYIETEFKYAPE I I I I I I I I I I I : I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m590-l TTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFAGGFGTQAYIETEFKYAPE 70 80 90 100 110 120 130 140 150 160 170 180 a590.pep TEKVLERFFGKQVPVSLANTVYFNGSGKMEVSVPAFDYEELSGIRLHWEGLTGETVYQKG I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m590-l TEKVLERFFGKQVPAΞLANTVYFNGSGKMEVSVPAFDYEELSGIRLHWEGLTGETVYQKG 130 140 150 160 170 180 190 200 210 220 230 240 a590.pep FKSYRNGYDAPLFKIKLADKGDAAFEKVHFDSETSDGINPLALGSSNLTLEKFSLEWKEG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m590-l FKSYRNGYDAPLFKIKLADKGDAAFEKVHFDSETSDGINPLALGSSNLTLEKFSLEWKEG 190 200 210 220 230 240 250 260 270 280 290 300 a590.pep VDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEVGKLAFSTKTGESGAFIDSEGQFRF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I m590-l VDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEVGKLAFSTKTGESGAFINSEGQFRF 250 260 270 280 290 300 310 320 330 340 350 360 a590.pep GTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKFARISAKKMTEEQIRNDLIAAVKGEAΞ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I m590-l DTLVYGDEKYGPLDIHIAAEHLDAΞALTVLKRKFAQISAKKMTEEQIRNDLIAAVKGEAΞ 310 320 330 340 350 360 370 380 390 400 410 420 a590.pep GLFTHNPVLDIKTFRFTLPΞGKIDVGGKIMFKDMKKEDLNQLGLMLKKTEADIRMSIPQK I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m590-l GLFTNNPVLDIKTFRFTLPΞGKIDVGGKIMFKDMKKEDLNQLGLMLKKTEADIRMΞIPQK 370 380 390 400 410 420 430 440 450 460 470 480 a590.pep MLEDLAVSQAGNIFSVNAEDEAEGRAΞLDDINETLRLMVDSTVQSMAREKYLTLNGDQID I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m590-l MLEDLAVSQAGNIFSVNAEDEAEGRASLDDINETLRLMVDSTVQΞMAREKYLTLNGDQID 430 440 450 460 470 480 490 500 510 a590.pep TAISLKNNQLKLNGKTLQNEPEPDFDEGGMVSEPQQX II I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I m590-l TAIΞLKNNQLKLNGKTLQNEPEPDFDEGGMVSEPQQX 490 500 510 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1841>: g591. seq 1 TTGCAAACCC TTCTAGCTTT TATCTTCGCC ATCCTGATTT TGGTCAGCCT 51 GCACGAATTC GGACACTACA TCGTCGCCAG GTTGTGCGGC GTCAAGGTTG 101 TGCGTTTTTC CGTCGGCTTC GGCAAACCGT TTTTCACCCG AAAGCGCGGC 151 GACACCGAAT GGTGCCTCGC CCCGATTCCG TTGGGCGGCT ACGTCAAAAT 201 GGTCGATACG CGCGAAGGCG AAGTATCAGA AGCCGATTTA CCCTACGCTT 251 TTGACAAACA ACACCCCGCC AAGCGCATCG CCATCGTCGC CGCCGGTCCG 301 CTGACCAACC TCGCActggc ggTTTTGCTG TACGGACTGa gctTttcctt 351 cggcgtaaCC GAACTGCGGC CCtatgtcgg cacagtcgaA cccgacaccg 401 ttgccgCCCG CACCGGCTTC caaagcggcg acaaAATACa atccgtcaac 451 ggcgtTtccg tCCAAGACTG GAGCAGCGCG CAAACCGAAA TCGTcctcAA 501 CCTCGAAGCC Ggcaaagtcg ccgtcggcgT TCAGACGGCA TCGGGCGCGC 551 AAACCGTCCG CACCAtcgAT GCCGCAGGCA CGCCGGAAGC CGGTAAAATC 601 GCAAAAAACC AAGGCTACAT CGGACTGATG CCCTTTAAAA TCACAACCGT 651 TGCCGGCGGC GTGGAAAAAG GCAGCCCCGC CGAAAAAGCA GGCCTGAAAC 701 CGGGCGACAG GCTGACTGCC GCCGACGGCA AACCCATCGc ctcaTGGCAG 751 GAATGggcaa acctgACccg cCAAAGCCCg ggcAAAAAAA Tcaccctgac 801 ctacgAaCGC GCcggacaaa cccaTAccgc CGACATCCGC CccgATactg 851 TCGAAcagcc cgACCACACC CTGATCgggc gcgTCGGCCT CCGtccgcaG 901 CCGGACAGGG CGTGGGACGC GCAAATCCGC CGCAGCTACC GTCCGTCTGT 951 TGTCCGCGCA TTCGGCATGG GCTGGGAAAA AACCGTTTCC CACTCGTGGA 1001 CAACCCTCAA ATTTTTCGGC AAACTAATCA GCGGCAACGC CTCTGTCAGC 1051 CATATTTCCG GGCCGCTGAC CATTGCCGAC ATTGCCGGAC AGTCCGCCGA 1101 ACTCGGCTTG CAAAGTTATT TGGAATTTTT AGCGTTGGTC AGCATCAGCC 1151 TCGGCGTGCT GAACCTGCTG CCCGTCCCCG TTTTGGACGG CGGGCACCTC 1201 GTGTTTTATA CTGTCGAATG GATACGCGGC AAACCTTTGG GCGAACGTGT 1251 CCAAAACATC GGTTTGCGCT TCGGGCTCGC CCTGATGATG CTGATGATGG 1301 CGGCCGCCTT CTTCAACGAC GTTACCCGGC TGATCGGTTA G This corresponds to the amino acid sequence <SEQ ID 1842; ORF 591. ng>: g591.pep.. 1 LQTLLAFIFA ILILVSLHEF GHYIVARLCG VKWRFSVGF GKPFFTRKRG 51 DTEWCLAPIP LGGYVKMVDT REGEVSEADL PYAFDKQHPA KRIAIVAAGP 101 LTNLALAVLL YGLSFSFGVT ELRPYVGTVE PDTVAARTGF QSGDKIQSVN 151 GVSVQDWSSA QTEIVLNLEA GKVAVGVQTA SGAQTVRTID AAGTPEAGKI 201 AKNQGYIGLM PFKITTVAGG VEKGSPAEKA GLKPGDRLTA ADGKPIASWQ 251 EWANLTRQSP GKKITLTYER AGQTHTADIR PDTVEQPDHT LIGRVGLRPQ 301 PDRAWDAQIR RSYRPSWRA FGMGWEKTVS HSWTTLKFFG KLISGNASVS 351 HISGPLTIAD IAGQSAELGL QSYLEFLALV SISLGVLNLL PVPVLDGGHL 401 VFYTVEWIRG KPLGERVQNI GLRFGLALMM LMMAAAFFND VTRLIG* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1843>: m591. seq 1 TTGCACACCC TTCTAGCTTT TATCTTCGCC ATCCTGATTT TGGTCAGCCT 51 GCACGAGTTC GGACACTACA TCGTTGCCAG ATTGTGCGGC GTCAAAGTCG 101 TACGCTTTTC CGTCGGCTTC GGCAAACCGT TTTTCACCCG AAAGCGCGGC 151 GACACCGAAT GGTGCCTCGC CCCGATTCCG TTGGGCGGTT ACGTCAAAAT 201 GGTCGATACG CGCGAAGGCG AAGTATCAGA AGCCGATTTA CCCTACGCTT 251 TTGACAAACA ACACCCCGCC AAGCGCATCG CCATCGTCGC CGCCGGCCCA 301 CTGACCAACC TCGCACTGGC GGTTTTGCTG TACGGACTGA GCTTTTCCTT 351 CGGCGTAACC GAACTGCGCC CCTACGTCGG CACAGTCGAA CCCGACACCA 401 TTGCCGCCCG CGCCGGCTTC CAAAGCGGCG ACAAAATACA ATCCGTCAAC 451 GGCACACCCG TTGCAGATTG GGGCAGCGCG CAAACCGAAA TCGTCCTCAA 501 CCTCGAAGCC GGCAAAGTCG CCGTCGGCGT TCAGACGGCA TCGGGCGCGC 551 AAACCGTCCG CACCATCGAT GCCGCAGGCA CGCCGGAAGC CGGTAAAATC 601 GCAAAAAACC AAGGCTACAT CGGACTGATG CCCTTTAAAA TCACAACCGT 651 TGCCGGCGGC GTGGAAAAAG GCAGCCCCGC CGAAAAAGCA GGCCTGAAAC 701 CGGGCGACAG GCTGACTGCC GCCGACGGCA AACCCATCGC CTCATGGCAA 751 GAATGGGCAA ACCTGACCCG CCAAAGCCCC GGCAAAAAAA TCACCCTGAA 801 CTACGAACGC GCCGGACAAA CCCATACCGC CGACATCCGC CCCGATACTG 851 TCGAACAGTC CGACCACACC CTGATCGGGC GCGTCGGCCT CCGTCCGCAG 901 CCGGACAGGG CGTGGGACGC GCAAATCCGC CGCAGCTACC GTCCGTCTGT 951 TGTCCGCGCA TTCGGCATGG GCTGGGAAAA AACCGTTTCC CACTCGTGGA 1001 CAACCCTCAA ATTTTTCGGC AAACTAATCA GCGGCAACGC CTCCGTCAGC 1051 CATATTTCCG GGCCGCTGAC CATTGCCGAC ATTGCCGGAC AGTCCGCCGA 1101 ACTCGGCTTG CAAAGTTATT TGGAATTTTT AGCACTGGTC AGCATCAGCC 1151 TCGGCGTGCT GAACCTACTG CCCGTCCCTG TTTTGGACGG CGGGCACCTC 1201 GTGTTTTATA CTGCCGAATG GATACGCGGC AAACCTTTGG GCGAACGCGT 1251 CCAAAACATC GGTTTGCGCT TCGGGCTCGC CCTCATGATG CTGATGATGG 1301 CGGTCGCCTT CTTCAACGAC GTTACCCGGC TGCTCGGTTA G This corresponds to the amino acid sequence <SEQ ID 1844; ORF 591>: m591 . pep . . 1 LHTLLAFIFA ILILVSLHEF _GHYIVARLCG VKWRFSVGF GKPFFTRKRG 51 DTEWCLAPIP LGGYVKMVDT REGEVSEADL PYAFDKQHPA KRIAIVAAGP 101 LTNLALAVLL YGLSFSFGVT ELRPYVGTVE PDTIAARAGF QSGDKIQSVN 151 GTPVADWGSA QTEIVLNLEA GKVAVGVQTA SGAQTVRTID AAGTPEAGKI 201 AKNQGYIGLM PFKITTVAGG VEKGSPAEKA GLKPGDRLTA ADGKPIASWQ 251 EWANLTRQSP GKKITLNYER AGQTHTADIR PDTVEQSDHT LIGRVGLRPQ 301 PDRAWDAQIR RSYRPSWRA FGMGWEKTVS HSWTTLKFFG KLISGNASVS 351 HISGPLTIAD IAGQSAELGL QSYLEFLALV SISLGVLNLL PVPVLDGGHL 401 VFYTAEWIRG KPLGERVQNI GLRFGLALMM LMMAVAFFND VTRLLG* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m591 / g591 97.3% identity in 446 aa overlap 10 20 30 40 50 60 m591. ■ pep LHTLLAFIFAILILVSLHEFGHYIVARLCGVKWRFSVGFGKPFFTRKRGDTEWCLAPIP M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M g591 LQTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPFFTRKRGDTEWCLAPIP 10 20 30 40 50 60 70 80 90 100 110 120 m591. .pep LGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLTΝLALAVLLYGLSFSFGVT II I II M II II II II II II II II II II II I II I I I I I I I I lllllllllllllll g591 LGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLTΝLALAVLLYGLSFSFGVT 70 80 90 100 110 120 130 140 150 160 170 180 m591. pep ELRPYVGTVEPDTIAARAGFQSGDKIQSVΝGTPVADWGSAQTEIVLΝLEAGKVAVGVQTA II II II II II II M II IM II I I I II I I II I: I II: I II II I I II I II II II I II I II g591 ELRPYVGTVEPDTVAARTGFQSGDKIQSVΝGVSVQDWSSAQTEIVLΝLEAGKVAVGVQTA 130 140 150 160 170 180 190 200 210 220 230 240 m591.pep SGAQTVRTIDAAGTPEAGKIAKΝQGYIGLMPFKITTVAGGVEKGSPAEKAGLKPGDRLTA II I I I II II II II II II II II II II II II I II I I I II I II I I I I I I I I I M I I I I I M M g591 SGAQTVRTIDAAGTPEAGKIAKΝQGYIGLMPFKITTVAGGVEKGSPAEKAGLKPGDRLTA 190 200 210 220 230 240 250 260 270 280 290 300 m591.pep ADGKPIASWQEWAΝLTRQSPGKKITLΝYERAGQTHTADIRPDTVEQSDHTLIGRVGLRPQ I M I II II I II II II II II II II I I MM I II II II II I M I I M I lllllllllllll g591 ADGKPIASWQEWAΝLTRQSPGKKITLTYERAGQTHTADIRPDTVEQPDHTLIGRVGLRPQ 250 260 270 280 290 300 310 320 330 340 350 360 m591.pep PDRAWDAQIRRSYRPSVVRAFGMGWEKTVSHSWTTLKFFGKLISGΝASVSHISGPLTIAD M II I I I II II II II II II I II II I II I I M M I I I M I I I I M M M M M I g591 PDRAWDAQIRRSYRPSWRAFGMGWEKTVSHSWTTLKFFGKLISGΝASVSHISGPLTIAD 310 320 330 340 350 360 370 380 390 400 410 420 m591.pep IAG Q SAELGL Q SYLEFLALVSISLGVLΝLLPVPVLDGGHLVFYTAEWIRGKPLGERVQΝI I I II II I II II I II II II II II I I II I I II I II I I I I II II I I I: I I I I II II I II II II g591 IAG Q SAELGL Q SYLEFLALVSISLGVLΝLLPVPVLDGGHLVFYTVEWIRGKPLGERVQΝI 370 380 390 400 410 420 430 440 m591. pep GLRFGLALMMLMMAVAFFΝDVTRLLGX II II I II II II II I : I II II II II : II g591 GLRFGLALMMLMMAAAFFΝDVTRLIGX 430 440 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1845>: a591 . seq 1 TTGCACACCC TTCTAGCTTT TATCTTCGCC ATCCTGATTT TGGTCAGCCT 51 GCACGAATTC GGACACTACA TCGTCGCCAG ATTGTGCGGC GTCAAGGTTG 101 TGCGTTTTTC CGTCGGCTTC GGCAAACCGT TTTTCACCCG AAAGCGCGGC 151 GACACCGAAT GGTGCCTCGC CCCGATTCCG TTGGGCGGTT ACGTCAAAAT 201 GGTCGACACG CGCGAAGGCG AAGTATCAGA AGCCGATTTA CCCTACGCTT 251 TTGACAAACA ACACCCCGCC AAGCGCATCG CCATCGTCGC CGCCGGCCCG 301 CTGACCAACC TCGCACTGGC GGTTTTGCTG TACGGACTGA GCTTTTCCTT 351 CGGCGTTACC GAACTGCGCC CCTATGTCGG CACAGTCGAA CCCGACACCA 401 TTGCCGCCCG CGCCGGCTTC CAAAGCGGCG ACAAAATACA ATCCGTCAAC 451 GGCACACCCG TTGCAGATTG GGGCAGCGCG CAAACCGAAA TCGTCCTCAA 501 CCTCGAAGCC GGCAAAGTCG CCGTCGGCGT TCAGACGGCA TCGGGCGCGC 551 AAACCGTCCG CACCATCGAT GCCGCAGGCA CGCCGGAAGC CGGTAAAATC 601 GCAAAAAACC AAGGCTACAT CGGACTGATG CCCTTTAAAA TCACAACCGT 651 TGCCGGCGGC GTGGAAAAAG GCAGCCCCGC CGAAAAAGCA GGCCTGAAAC 701 CGGGCGACAG GCTGACTGCC GCCGACGGCA AACCCATCGC CTCATGGCAA 751 GAATGGGCAA ACCTGACCCG CCAAAGCCCC GGCAAAAAAA TCACCCTGAC 801 CTACGAACGC GCCGGACAAA CCCATACCGC CGACATCCGC CCCGATACTG 851 TCGAACAGCC CGACCACACC CTGATCGGGC GCGTCGGCCT CCGTCCGCAG 901 CCGGACAGGG CGTGGGACGC GCAAATCCGC CGCAGCTACC GTCCGTCTGT 951 TGTCCGCGCA TTCGGCATGG GCTGGGAAAA AACCGTTTCC CACTCGTGGA 1001 CAACCCTCAA ATTTTTCGGC AAACTAATCA GCGGCAACGC CTCCGTCAGC 1051 CATATTTCCG GTCCGCTGAC CATTGCCGAT ATTGCCGGAC AGTCCGCCGA 1101 ACTCGGCTTG CAAAGTTATT TGGAATTTTT GGCACTGGTC AGCATCAGCC 1151 TCGGCGTGCT GAACCTGCTG CCCGTCCCCG TTTTGGACGG CGGCCACCTC 1201 GTGTTTTATA CTGCCGAATG GATACGCGGC AAACCTTTGG GCGAACGCGT 1251 CCAAAACATC GGTTTGCGCT TCGGGCTTGC CCTCATGATG CTGATGATGG 1301 CGGTCGCCTT CTTCAACGAC GTTACCCGGC TGCTCGGTTA G This corresponds to the amino acid sequence <SEQ ID 1846; ORF 591. a>: a591.pep 1 LHTLLAFIFA ILILVSLHEF GHYIVARLCG VKWRFSVGF GKPFFTRKRG 51 DTEWCLAPIP LGGYVKMVDT REGEVSEADL PYAFDKQHPA KRIAIVAAGP 101 LTNLALAVLL YGLSFSFGVT ELRPYVGTVE PDTIAARAGF QSGDKIQSVN 151 GTPVADWGSA QTEIVLNLEA GKVAVGVQTA SGAQTVRTID AAGTPEAGKI 201 AKNQGYIGLM PFKITTVAGG VEKGSPAEKA GLKPGDRLTA ADGKPIASWQ 251 EWANLTRQSP GKKITLTYER AGQTHTADIR PDTVEQPDHT LIGRVGLRPQ 301 PDRAWDAQIR RSYRPSWRA FGMGWEKTVS HSWTTLKFFG KLISGNASVS 351 HISGPLTIAD IAGQSAELGL QSYLEFLALV SISLGVLNLL PVPVLDGGHL 401 VFYTAEWIRG KPLGERVQNI GLRFGLALMM LMMAVAFFND VTRLLG* m591/a591 99.6% identity in 446 aa overlap 10 20 30 40 50 60 m591. pep LHTLLAFIFAILILVSLHEFGHYIVARLCGVKWRFSVGFGKPFFTRKRGDTEWCLAPIP II II I II II I II II II I II II II II II I II II II II I II II II II II I I II II II II II I a591 LHTLLAFIFAILILVΞLHEFGHYIVARLCGVKWRFSVGFGKPFFTRKRGDTEWCLAPIP 10 20 30 40 50 60 70 80 90 100 110 120 m591. pep LGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLTNLALAVLLYGLSFSFGVT II II II I II I II II II I II II II I II II II I II I I II II I II I II I II I II II I I M I I I a591 LGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLTNLALAVLLYGLSFSFGVT 70 80 90 100 110 120 130 140 150 160 170 180 m591.pep ELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSAQTEIVLNLEAGKVAVGVQTA II II II II II I I II II II II II II II I II I II I I I I I I I II I II I I II II II II I M I I I a591 ELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSAQTEIVLNLEAGKVAVGVQTA 130 140 150 160 170 180 190 200 210 220 230 240 m591.pep SGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVAGGVEKGSPAEKAGLKPGDRLTA I II II II II I II I II II II II II I I II II II II I I II I I M M I I I M I I M I M I I M I a591 SGA Q TVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVAGGVEKGSPAEKAGLKPGDRLTA 190 200 210 220 230 240 250 260 270 280 290 300 m591.pep ADGKPIASWQEWANLTRQSPGKKITLNYERAGQTHTADIRPDTVEQSDHTLIGRVGLRPQ I II II II II II II II II II II II II I : II II II I II I II II I II II lllllllllllll a591 ADGKPIASWQEWANLTRQSPGKKITLTYERAGQTHTADIRPDTVEQPDHTLIGRVGLRPQ 250 260 270 280 290 300 310 320 330 340 350 360 m591.pep PDRAWDAQIRRSYRPSWRAFGMGWEKTVSHSWTTLKFFGKLISGNASVSHISGPLTIAD I II II II II II II II II II II II II II II I II I II II II II II II II II II II II II II I a591 PDRAWDAQIRRSYRPSWRAFGMGWEKTVSHSWTTLKFFGKLISGNASVSHISGPLTIAD 310 320 330 340 350 360 370 380 390 400 410 420 m591. pep IAGQSAELGLQSYLEFLALVSISLGVLNLLPVPVLDGGHLVFYTAEWIRGKPLGERVQNI II II II II II II II II II II II II II II II II I II II II I II II II II I I I I II II II II a591 IAGQSAELGLQSYLEFLALVSISLGVLNLLPVPVLDGGHLVFYTAEWIRGKPLGERVQNI 370 380 390 400 410 420 430 440 m591. pep GLRFGLALMMLMMAVAFFNDVTRLLGX II II II II II I II II II II II II II II a591 GLRFGLALMMLMMAVAFFNDVTRLLGX 430 440 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1847>: g592.seq.. 1 atgattccgg acgtgttcgg tcagattttt tcgggcgcgt tcaaattcga 51 cgcggcagca ggcggcttac tcggcggtct gatttcgcaa acgatgatga 101 tgggcatcaa acgcggcctg tattccaacg aggcgggtat gggttccgcg 151 ccgaacgccg ccgccgcogc cgaagtgaaa caccctgttt cgcaaggtat 201 gattcaaatg ctgggcgtgt ttgtcgatac catcatcgtt tgttcttgca 251 ccgccttcat catcttgatt taccaacagc cttatggcga tttgagcggt 301 gcggcgctga cgcaggcggc gattgtcagc caagtggggc aatggggcgc 351 gggtttcctc gccgtcatcc tgtttatgtt tgccttttcc accgttatcg 401 gcaactatgc ctatgccgag tccaacgtcc aattcatcaa aagccattgg 451 ctgattaccg ccgttttccg tatgctggtt ttggcgtggg tctatttcgg 501 cgcggttgcc aatgtgcctt tggtctggga tatggcggat atggcgatgg 551 gcatcatggc gtggatcaac ctcgtcgcca tcctgctgct ctcgccattg 601 gcgtttatgc tgctgcgcga ttacaccgcc aagctgaaaa tgggcaaaga 651 ccccgagttc aaactttccg aacatccggg cctgaaacgc cgcatcaaat 701 ccgatgtttg gtaa This corresponds to the amino acid sequence <SEQ ID 1848; ORF 592.ng>: g592.pep .. 1 MIPDVFGQIF SGAFKFDAAA GGLLGGLISQ TMMMGIKRGL YSΝEAGMGSA 51 PΝAAAAAEVK HPVSQGMIQM LGVFVDTIIV CΞCTAFIILI YQQPYGDLSG 101 AALTQAAIVS QVGQWGAGFL AVILFMFAFS TVIGΝYAYAE SΝVQFIKSHW 151 LITAVFRMLV LAWVYFGAVA ΝVPLVWDMAD MAMGIMAWIΝ LVAILLLSPL 201 AFMLLRDYTA KLKMGKDPEF KLSEHPGLKR RIKSDVW* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1849>: m592.seq 1 ATGATTCCGG ACGTGTTCGG TCAGATTTTT TCGGGCGCGT TCAAATTCGA 51 CGCGGCAGCA GGCGGCTTAC TCGGCGGTCT GATTTCGCAA ACGATGATGA 101 TGGGCATCAA ACGCGGCCTG TATTCCAACG AGGCGGGTAT GGGTTCCGCG 151 CCGAACGCCG CCGCCGCCGC CGAAGTGAAA CACCCTGTTT CGCAAGGTAT 201 GATTCAAATG CTGGGCGTGT TTGTCGATAC CATCATCGTT TGTTCTTGCA 251 CCGCCTTCAT CATCTTGATT TACCAACAGC CTTACGGCGA TTTGAGCGGT 301 GCGGCGCTGA CGCAGGCGGC GATTGTCAGC CAAGTGGGGC AATGGGGCGC 351 GGGCTTCCTC GCCGTCATCC TGTTTATGTT TGCCTTTTCC ACCGTTATCG 401 GCAACTATGC CTATGCCGAG TCCAACGTCC AATTCATCAA AAGCCATTGG 451 CTGATTACCG CCGTTTTCCG TATGCTGGTT TTGGCGTGGG TCTATTTCGG 501 CGCGGTTGCC AATGTGCCTT TGGTCTGGGA TATGGCGGAT ATGGCGATGG 551 GCATTATGGC GTGGATCAAC CTTGTCGCCA TCCTGCTGCT CTCGCCCTTG 601 GCGTTTATGC TGCTGCGCGA TTACACCGCC AAGCTGAAAA TGGGCAAAGA 651 CCCCGAGTTC AAACTTTCCG AACATCCGGG CCTGAAACGC CGTATCAAAT 701 CCGACGTTTG GTAA This corresponds to the amino acid sequence <SEQ ID 1850; ORF 592>: m592.pep 1 MIPDVFGQIF SGAFKFDAAA GGLLGGLISQ TMMMGIKRGL YSNEAGMGSA 51 PNAAAAAEVK HPVSQGMIQM LGVFVDTIIV CSCTAFIILI YQQPYGDLSG 101 AALTQAAIVS QVGQWGAGFL AVILFMFAFS TVIGNYAYAE SNVQFIKSHW 151 LITAVFRMLV LAWVYFGAVA NVPLVWDMAD MAMGIMAWIN LVAILLLSPL 201 AFMLLRDYTA KLKMGKDPEF KLSEHPGLKR RIKSDVW* m592 / g592 100.0% identity in 237 aa overlap 10 20 30 40 50 60 m592 pep MIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKRGLYSNEAGMGSAPNAAAAAEVK I ! 111 ! 111 II I II I II 11 ! 11111111 ! 11111 Ii I E I II 11 i 111 i 1111 II 111111 g 59 2 MIPDVFGQIFSGAFKFDAAAGGLLGGLIΞQTMMMGIKRGLYSNEAGMGSAPNAAAAAEVK 10 20 30 40 50 60 70 80 90 100 110 120 m592. pep HPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYGDLSGAALTQAAIVSQVGQWGAGFL I M I I I I I I I I M I M M M M I M M M I I I I M I I I I I I I I 1 1 1 1 I I I M I I M I M I g 92 HPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYGDLSGAALTQAAIVSQVGQWGAGFL 70 80 90 100 110 120 130 140 150 160 170 180 m592 pep AVILFMFAFSTVIGNYAYAESNVQFIKSHWLITAVFRMLVLAWVYFGAVANVPLVWDMAD 111 j 111111111 M I II ! 1111111111 i 1111111111111111111111 M 111111 g592 AVILFMFAFSTVIGNYAYAESNVQFIKSHWLITAVFRMLVLAWVYFGAVANVPLVWDMAD 130 140 150 160 170 180 190 200 210 220 230 m592.pep MAMGIMAWINLVAILLLSPLAFMLLRDYTAKLKMGKDPEFKLSEHPGLKRRIKSDVWX II III II III 1111 III II g592 MAMGIMAWINLVAILLLSPLAFMLLRDYTAKLKMGKDPEFKLSEHPGLKRRIKSDVWX 190 200 210 220 230 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1851>: a592.seq 1 ATGATTCCGG ACGTGTTCGG TCAGATTTTT TCGGGCGCGT TCAAATTCGA 51 CGCGGCAGCA GGCGGCTTAC TCGGCGGTCT GATTTCGCAA ACGATGATGA 101 TGGGCATCAA ACGCGGCCTG TATTCCAACG AGGCGGGTAT GGGTTCCGCG 151 CCGAACGCCG CCGCCGCCGC CGAAGTGAAA CACCCTGTTT CGCAAGGTAT 201 GATTCAAATG CTGGGCGTGT TTGTCGATAC CATCATCGTT TGTTCTTGCA 251 CCGCCTTCAT CATCTTGATT TACCAACAGC CTTACGGCGA TTTGAGCGGT 301 GCGGCGCTGA CGCAGGCGGC GATTGTCAGC CAAGTGGGGC AATGGGGCGC 351 GGGCTTCCTC GCCGTCATCC TGTTTATGTT TGCCTTTTCC ACCGTTATCG 401 GCAACTATGC CTATGCCGAG TCCAACGTCC AATTCATCAA AAGCCATTGG 451 CTGATTACCG CCGTTTTCCG TATGCTGGTT TTGGCGTGGG TCTATTTCGG 501 CGCGGTTGCC AATGTGCCTT TGGTCTGGGA TATGGCGGAT ATGGCGATGG 551 GCATTATGGC GTGGATCAAC CTTGTCGCCA TCCTGCTGCT CTCGCCCTTG 601 GCGTTTATGC TGCTGCGCGA TTACACCGCC AAGCTGAAAA TGGGCAAAGA 651 CCCCGAGTTC AAACTTTCCG AACATCCGGG CCTGAAACGC CGTATCAAAT 701 CCGACGTTTG GTAA This corresponds to the amino acid sequence <SEQ ID 1852; ORF 592.a>: a592.pep 1 MIPDVFGQIF SGAFKFDAAA GGLLGGLISQ TMMMGIKRGL YSΝEAGMGSA 51 PΝAAAAAEVK HPVSQGMIQM LGVFVDTIIV CSCTAFIILI YQQPYGDLSG 101 AALTQAAIVS QVGQWGAGFL AVILFMFAFS TVIGΝYAYAE SΝVQFIKSHW 151 LITAVFRMLV LAWVYFGAVA ΝVPLVWDMAD MAMGIMAWIΝ LVAILLLSPL 201 AFMLLRDYTA KLKMGKDPEF KLSEHPGLKR RIKSDVW* m592/a592 100.0% identity in 237 aa overlap 10 20 30 40 50 60 m592. pep MIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKRGLYSNEAGMGSAPNAAAAAEVK II I II I II II I II II II II II II I II II II II II II II II I II II II I II II I II II II I a592 MIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKRGLYSNEAGMGSAPNAAAAAEVK 10 20 30 40 50 60 70 80 90 100 110 120 m592.pep HPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYGDLSGAALTQAAIVSQVGQWGAGFL MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a592 HPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYGDLSGAALTQAAIVSQVGQWGAGFL 70 80 90 100 110 120 130 140 150 160 170 180 m592. pep AVILFMFAFSTVIGNYAYAESNVQFIKSHWLITAVFRMLVLAWVYFGAVANVPLVWDMAD I II II I II I II II II II I II I I II I II II II I II II II I II II II II II II II II II II I a592 AVILFMFAFSTVIGNYAYAESNVQFIKSHWLITAVFRMLVLAWVYFGAVANVPLVWDMAD 130 140 150 160 170 180 190 200 210 220 230 m592.pep MAMGIMAWINLVAILLLSPLAFMLLRDYTAKLKMGKDPEFKLSEHPGLKRRIKSDVWX I II I II I II II II II II I II II II II I II II II II II II II II II II II II II II I I I a592 MAMGIMAWINLVAILLLSPLAFMLLRDYTAKLKMGKDPEFKLSEHPGLKRRIKSDVWX 190 200 210 220 230 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1853>: g593. seq.. 1 atgcttgaac tgaacggact ctgcaaatgc ttcggcggca aaacggtcgc 51 cgacaacatc tgcctgactg tcgggcgcgg caaaatactc gccgtactgg 101 ggcggtcggg ctgcggcaaa tccaccctgc tgaatatgat tgcgggcatc 151 gtccggccgg acggcggcga aattcggctg aacggggaaa acattacctg 201 tatgccgccc gaaaaacgcc gtatctcgct gatgtttcaa gattacgcgc 251 tgtttcccca tatgagtgcg ctggaaaata cggcattcgg tttgaaaatg 301 caaaaaatgc cgaaagccga agccgaacgc ctcgccttgt cggcacttgc 351 cgaagtcggg ctggaaaacg aggcgcaccg caagcctgaa aaactttccg 401 gaggcgagaa gcaacggttg gcactggcgc gcgctttggt tgtccgccct 451 tccctgctgt tgctggatga atcgttttcc agtttggaca cgcatttgcg 501 cgaccggctg cgccgtatga ccgccgaacg catccgcaag ggcggcatcc 551 ctgccgtttt ggtaacgcat tcgcccgaag aggcctgcac ggcggcggac 601 gaaatcgccg tcatgcacga ggggaaaatc cttcaatgcg gtacgcccga 651 aaccttgatt caaacgcctg ccggcgtgca ggtcgcccgt ctgatggggc 701 tgcccaatac cgacgatgac cgccatattc cgcaaaatgc cgtgtgcttg 751 gacaatcatg gaacggaatg ccgtctgctg tccctcgtcc gcctgcccga 801 ctcgctccgg ctttccgccg tccatcccga acacggcgag ctgaccttaa 851 acctgactgt cggacaacat acggacggta tttccggaaa cggtacggtc 901 cgcatccgcg tcgatgaagg gcgtatcgtc cgtttccgat ga This corresponds to the amino acid sequence <SEQ ID 1854; ORF 593.ng>: g593.pep.. 1 MLELΝGLCKC FGGKTVADΝI CLTVGRGKIL AVLGRSGCGK STLLΝMIAGI 51 VRPDGGEIRL ΝGEΝITCMPP EKRRISLMFQ DYALFPHMSA LEΝTAFGLKM 101 QKMPKAEAER LALSALAEVG LEΝEAHRKPE KLSGGEKQRL ALARALWRP 151 SLLLLDESFS SLDTHLRDRL RRMTAERIRK GGIPAVLVTH SPEEACTAAD 201 EIAVMHEGKI LQCGTPETLI QTPAGVQVAR LMGLPΝTDDD RHIPQΝAVCL 251 DΝHGTECRLL SLVRLPDSLR LSAVHPEHGE LTLΝLTVGQH TDGISGΝGTV 301 RIRVDEGRIV RFR* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1855>: m593. seq 1 ATGCTTGAAC TGAACGGACT CTGCAAACGC TTCGGCAATA AAACCGTCGC 51 CGACAACATC TGCCTGACTG TCGGGCGCGG CAAAATACTC GCCGTTTTGG 101 GGCGGTCGGG CTGCGGAAAA TCCACCCTGC TGAATATAAT TGCGGGGATT 151 GTCCGGCCGG ACGGCGGGGA AATATGGCTG AACGGAGAAA ACATTACCCG 201 TATGCCGCCC GAAAAACGCC GTATCTCGCT GATGTTTCAA GATTACGCGC 251 TGTTTCCCCA TATGAGTGCG CTGGAAAATG CGGCATTCGG TTTGAAAATG 301 CAAAAAATGC CGAAAGCCGA AGCCGAACGC CTCGCCATGG CGGCACTTGC 351 CGAAGTCGGA CTGGAAAACG AGGCGCACCG CAAGCCTGAA AAACTTTCCG 401 GAGGCGAGAA GCAACGGCTG GCGTTGGCGC GCGCTTTGGT TGTCCGCCCT 451 TCCCTGCTGC TGTTGGACGA ATCGTTTTCC AGTTTGGACA CGCATTTGCG 501 CGGCACGCTG CGCCGTATGA CTGCCGAACG TATCCGAAAC GGCGGCATCC 551 CTGCCGTTTT GGTAACGCAT TCGCCCGAAG AAGCCTGTAC GACGGCAGAC 601 GAAATCGCCG TGATGCATAA AGGGAGGATT CTACAATACG GTACGCCCGA 651 AACATTGGTC AAAACACCAT CCTGCGTGCA GGTCGCCCGA CTGATGGGTT 701 TGCCCAATAC CGACGATAAC CGCCATATTC CGCAACATGC GGTGCGTTTC 751 GACCAAGACG GCATGGAGTG CCGCGTATTA TCCCGTACCT GTTTGCCCGA 801 ATCGTTCAGC CTGTCCGTCC TCCATCCGGA ACACGGCATC CTGTGGCTGA 851 ACCTCGATAT GCGGCACGCC GGGGCGGTAT CGGGCAAGGA TACGGTACGC 901 ATCCATATCG AAGAACGGGA AATCGTCCGC TTCCGCTGA This corresponds to the amino acid sequence <SEQ ID 1856; ORF 593>: m593.pep 1 MLELNGLCKR FGNKTVADNI CLTVGRGKIL AVLGRSGCGK STLLNIIAGI 51 VRPDGGEIWL NGENITRMPP EKRRISLMFQ DYALFPHMSA LENAAFGLKM 101 QKMPKAEAER LAMAALAEVG LENEAHRKPE KLSGGEKQRL ALARALWRP 151 SLLLLDESFS SLDTHLRGTL RRMTAERIRN GGIPAVLVTH SPEEACTTAD 201 EIAVMHKGRI LQYGTPETLV KTPSCVQVAR LMGLPNTDDN RHIPQHAVRF 251 DQDGMECRVL SRTCLPESFS LSVLHPEHGI LWLNLDMRHA GAVSGKDTVR 301 IHIEEREIVR FR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m593 / g593 83.4% identity in 313 aa overlap 10 20 30 40 50 60 m593.pep MLELΝGLCKRFGΝKTVADΝICLTVGRGKILAVLGRSGCGKSTLLΝIIAGIVRPDGGEIWL lllllllll II M II I II II II I II II II I II II II II II II II : II II II II II II I g593 MLELΝGLCKCFGGKTVADΝICLTVGRGKILAVLGRSGCGKSTLLΝMIAGIVRPDGGEIRL 10 20 30 40 50 60 70 80 90 100 110 120 m593.pep ΝGEΝITRMPPEKRRISLMFQDYALFPHMSALEΝAAFGLKMQKMPKAEAERLAMAALAEVG llllll II II II II I II II II I II II II II II : I II II I II I I I II I II II : : I I II I I g593 ΝGEΝITCMPPEKRRISLMFQDYALFPHMSALEΝTAFGLKMQKMPKAEAERLALSALAEVG 70 80 90 100 110 120 130 140 150 160 170 180 m593.pep LEΝEAHRKPEKLSGGEKQRLALARALWRPSLLLLDESFSSLDTHLRGTLRRMTAERIRΝ II II I II II II II II II II II II II I II II II II II II I II I II II I llllllllll: g593 LEΝEAHRKPEKLSGGEKQRLALARALWRPSLLLLDESFSSLDTHLRDRLRRMTAERIRK 130 140 150 160 170 180 190 200 210 220 230 240 m593.pep GGIPAVLVTHSPEEACTTADEIAVMHKGRILQYGTPETLVKTPSCVQVARLMGLPΝTDDΝ II II II I I II II II II I : I I I II II I : I M II II I II |:: II : llllllllllllll: g593 GGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPETLIQTPAGVQVARLMGLPΝTDDD 190 200 210 220 230 240 250 260 270 280 290 299 m593.pep RHIPQHAVRFDQDGMECRVLSRTCLPESFSLSVLHPEHGILWLNLDM-RHAGAVSGKDTV IIIII I : I : I M M : MM: I I : : II II I I III : : I : : : I I : II g593 RHIPQNAVCLDNHGTECRLLSLVRLPDSLRLSAVHPEHGELTLNLTVGQHTDGISGNGTV 250 260 270 280 290 300 300 310 m593.pep RIHIEEREIVRFRX IM:: MIIIM g593 RIRVDEGRIVRFRX 310 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1857>: a593.seq 1 ATGCTTGAAC TGAACGGACT CTGCAAACGC TTCGGCGGCA AAACGGTTGC 51 CGACGATATC TGCCTGACTG TCGGGCGCGG CAAAATACTC GCCGTTTTGG 101 GGCGGTCGGG CTGCGGCAAA TCCACCCTGC TGAATATGAT TGCGGGCATC 151 GTCCGGCCGG ACGGCGGGGA AATATGGCTG AATGGGGAAA ACATTACCCG 201 TATGCCGCCC GAAAAACGCC GTATTTCGCT GATGTTTCAA GATTACGCGC 251 TGTTTCCCCA TATGAGTGCA CTGGAAAATG CGGCATTCGG TTTGAAAATG 301 CAAAAAATGC CGAAAGCCGA AGCCGAAAGC CTCGCCATGG CGGCACTTGC 351 CGAAGTCGGA CTGGAAAACG AGGCGCACCG CAAGCCTGAΝ AAACTTTCCG 401 GAGGCGAAAA GCAACGGTTG GCACTGGCGC GCGCTTTGGT TGTCCGCCCT 451 TCCCTGCTGC TGTTGGACGA ATCGTTTTCC AGTTTGGACA CGCATTTGCG 501 CGACCGGCTG CGCCGCATGA CTGCCGAACG TATCCGCAAG GGCGGCATCC 551 CTGCCGTTTT GGTAACGCAT TCGCCCGAAG AGGCCTGCAC GGCGGCAGAC 601 GAAATCGCCG TCATGCACGA GGGGAAAATC CTTCAATGCG GTACGCCCGA 651 AACCTTGGTT CAAACGCCTG CCGGCGTGCA GGTCGCCCAT CTGATGGGGC 701 TGCCCAATAC CGACGATGAC CGCCATATTC CGCAACATGC GGTGCGTTTC 751 GACCAAGACG GCATGGAGTG CCGCGTATTA TCCCGTACCT GTTTGCCCGA 801 ATCGTTCAGC CTGTCCGTCC TCCATCCGGA ACACGGCATC CTGTGGCTGA 851 ACCTCGATAT GCCGCACGCC GGTGAAATAT CGGGAAACGA TACGGTACGC 901 ATCCATATCG AAGACAGGGA AATCGTCCGC TTCCGCTGA This corresponds to the amino acid sequence <SEQ ID 1858; ORF 593. a>: a593.pep 1 MLELNGLCKR FGGKTVADDI CLTVGRGKIL AVLGRSGCGK ΞTLLNMIAGI 51 VRPDGGEIWL NGENITRMPP EKRRISLMFQ DYALFPHMSA LENAAFGLKM 101 QKMPKAEAES LAMAALAEVG LENEAHRKPX KLSGGEKQRL ALARALWRP 151 SLLLLDESFS SLDTHLRDRL RRMTAERIRK GGIPAVLVTH SPEEACTAAD 201 EIAVMHEGKI LQCGTPETLV QTPAGVQVAH LMGLPNTDDD RHIPQHAVRF 251 DQDGMECRVL SRTCLPESFS LSVLHPEHGI LWLNLDMPHA GEISGNDTVR 301 IHIEDREIVR FR* m593/a593 92.9% identity in 312 aa overlap 10 20 30 40 50 60 m593.pep MLELNGLCKRFGNKTVADNICLTVGRGKILAVLGRSGCGKSTLLNIIAGIVRPDGGEIWL II I II II II II I : I I I I I: I II II I I II II II II II II I II II II: II II II I I II I II I a593 MLELNGLCKRFGGKTVADDICLTVGRGKILAVLGRSGCGKSTLLNMIAGIVRPDGGEIWL 10 20 30 40 50 60 70 80 90 100 110 120 m593. ep NGENITRMPPEKRRISLMFQDYALFPHMΞALENAAFGLKMQKMPKAEAERLAMAALAEVG II I II I I II II II II II I II II II II II II II I I I I I II I I I II I I I I I llllllllll a593 NGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQKMPKAEAEΞLAMAALAEVG 70 80 90 100 110 120 130 140 150 160 170 180 m593.pep LENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFSSLDTHLRGTLRRMTAERIRN II I II I II I II II II II I I II II I II I II I I I II I I I I I II I I II I llllllllll: a593 LENEAHRKPXKLSGGEK Q RLALARALWRPSLLLLDESFSSLDTHLRDRLRRMTAERIRK 130 140 150 160 170 180 190 200 210 220 230 240 m593.pep GGIPAVLVTHSPEEACTTADEIAVMHKGRILQYGTPETLVKTPSCVQVARLMGLPNTDDN II II II I I II II II II I : I II I I II I : I : I I I I II II II M I : II II : I I II I I II I : a593 GGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPETLVQTPAGVQVAHLMGLPNTDDD 190 200 210 220 230 240 250 260 270 280 290 300 m593.pep RHIPQHAVRFDQDGMECRVLSRTCLPESFSLSVLHPEHGILWLNLDMRHAGAVSGKDTVR II I I II II I II II I I II I II II I I II I II II I II II I II II II II II III : II : I I I I a593 RHIPQHAVRFDQDGMECRVLSRTCLPESFSLSVLHPEHGILWLNLDMPHAGEISGNDTVR 250 260 270 280 290 300 310 m593.pep IHIEEREIVRFRX II II : I II II II I a593 IHIEDREIVRFRX 310 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1859>: g594. seq.. 1 atgggtgcag ataccgatgg cgacaaggat gttcggctta atcgaacggg 51 tctcgttttt agcatactcc ggctgctgtt ccgcatcgga attgggatcg 101 gtaagttcgc cgttcaggcc tttcaggtct ttaagctgct gatctgtacg 151 gttgagcacc caaatcggtt tgccttgcca ctcggcggtc agcagctgac 201 ccgcttcgat tttactgaca tccacctcga cggcagcacc ggaggccttg 251 gctttttccg aagggaaaaa actggccaca aacggcgttg ccacacccaa 301 tgctgccact ccgcccgcgc cgcaggtcgc aagtgtcagg aaacggcggc 351 ggccgttgtt gatttcttga ttatccatta ttcagtcgtc ctaatatttt 401 gggaatgccg agccattaaa cattgcaatt ttacccagtt tgcagtgata 451 ctcaaagcat tatttaaaat aaggtaa This corresponds to the amino acid sequence <SEQ ID 1860; ORF 594.ng>: g594.pep 1 MGADTDGDKD VRLΝRTGLVF SILRLLFRIG IGIGKFAVQA FQVFKLLICT 51 VEHPΝRFALP LGGQQLTRFD FTDIHLDGST GGLGFFRREK TGHKRRCHTQ 101 CCHSARAAGR KCQETAAAW DFLIIHYSW LIFWECRAIK HCΝFTQFAVI 151 LKALFKIR* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1861>: m594. seq 1 ATGGGTGCAG ATACCGATGG CGACAAGGAT GTTCGGCTTA ATCGAACGGG 51 TCTCGTTTTT AGCATACTCC GGCTGCTGTT CCGCATCGGA ATTGGGATCG 101 GTAAGTTCGC CGTTCAGGCC TTTCAGGTCT TTAAGCTGCT GATCTGTACG 151 GTTGAGCACC CAAATCGGTT TGCCTTGCCA CTCGGCGGTC AGCAGCTGAC 201 CCGCTTCGAT TTTACTGACA TCCACCTCGA CGGCAGCACC GGCGGCCTTG 251 GCTTTTTCCG AAGGGAAAAA ACTGGCCACA AACGGCGTTG CCACACCCAA 301 TGCTGCCACT CCGCCCGCGC CGCAGGTCGC GAGTGTCAGG AAACGGCGGC 351 GGCCGTTGTT GATTTCTTGA TTATCCATTA TTCAGTCGTC CTAATATTTT 401 GGGAATACCG AGCCATTAAA CGTTGCAATT TTACCCAGTT TGCAGTGATA 451 CTCAAAGCAT TATTTAAAAT AAGGTAA This corresponds to the amino acid sequence <SEQ ID 1862; ORF 594>: m594.pep 1 MGADTDGDKD VRLΝRTGLVF SILRLLFRIG IGIGKFAVQA FQVFKLLICT 51 VEHPΝRFALP LGGQQLTRFD FTDIHLDGST GGLGFFRREK TGHKRRCHTQ 101 CCHSARAAGR ECQETAAAW DFLIIHYSW LI WEYRAIK RCΝFTQFAVI 151 LKALFKIR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m594 / g594 98.1% identity in 158 aa overlap 10 20 30 40 50 60 m594.pep MGADTDGDKDVRLNRTGLVFSILRLLFRIGIGIGKFAVQAFQVFKLLICTVEHPNRFALP I I II I II II II II II II I II II II II I II II I II II II I II II II I I I II I II II II I II g594 MGADTDGDKDVRLNRTGLVFSILRLLFRIGIGIGKFAVQAFQVFKLLICTVEHPNRFALP 10 20 30 40 50 60 70 80 90 100 110 120 m594. pep LGGQQLTRFDFTDIHLDGSTGGLGFFRREKTGHKRRCHTQCCHSARAAGRECQETAAAW MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g594 LGGQQLTRFDFTDIHLDGSTGGLGFFRREKTGHKRRCHTQCCHSARAAGRKCQETAAAW 70 80 90 100 110 120 130 140 150 159 m594.pep DFLIIHYSWLIFWEYRAIKRCNFTQFAVILKALFKIRX I II II II II II II II II II : I II I II I II II I I I I I I I g594 DFLIIHYSWLIFWECRAIKHCNFTQFAVILKALFKIRX 130 140 150 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 1863>: a594. seq 1 ATGGGTGCAG ATACCGATGG CGACAAGGAT GTTCGGCTTA ATCGAACGGG 51 TCTCGTTTTT AGCATACTCC GGCTGCTGTT CCGCATCGGA ATTGGGATCG 101 GTAAGTTCGC CGTTCAGGCC TTTCAGGTCT TTAAGCTGCT GATCTGTACG 151 GTTGAGCACC CAAATCGGTT TGCCTTGCCA CTCGGCGGTC AGCAACTGAC 201 CCGCTTCGAT TTTACTGACA TCCACCTCGA CGGCAGCACC GGCGGCCTTG 251 GCTTTTTCCG AAGGGAAAAA ACTGGCCACA AACGGCGTTG CCACACCCAA 301 TGCTGCCACT CCGCCCGCGC CGCAGGTCGC GAGTGTCAGG AAACGGCGGC 351 GGCCGTTGTT GATTTCTTGA TTATCCATTA TTCAGTCGTC CTAATATTTT 401 GGGAATACCG AGCCATTAAA CGTTGCAATT TTACCCAGTT TGCAGTGATA 451 CTCAAAGCAT TATTTAAAAT AAGGTAA This corresponds to the amino acid sequence <SEQ ID 1864; ORF 594. a>: a594.pep 1 MGADTDGDKD VRLΝRTGLVF SILRLLFRIG IGIGKFAVQA FQVFKLLICT 51 VEHPΝRFALP LGGQQLTRFD FTDIHLDGST GGLGFFRREK TGHKRRCHTQ 101 CCHSARAAGR ECQETAAAW DFLIIHYSW LIFWEYRAIK RCΝFTQFAVI 151 LKALFKIR* m594/a594 100.0% identity in 158 aa overlap 10 20 30 40 50 60 m594.pep MGADTDGDKDVRLΝRTGLVFSILRLLFRIGIGIGKFAVQAFQVFKLLICTVEHPΝRFALP I II II I II II I II II II II II II II II II I II II II I II II II II II II II I II I I I II I a594 MGADTDGDKDVRLΝRTGLVFSILRLLFRIGIGIGKFAVQAFQVFKLLICTVEHPΝRFALP 10 20 30 40 50 60 70 80 90 100 110 120 m59 .pep LGGQQLTRFDFTDIHLDGSTGGLGFFRREKTGHKRRCHTQCCHSARAAGRECQETAAAW II II II II II II II II I II II I I II II II II I II I I II I I II II I I I I II II I I M I II I a594 LGGQQLTRFDFTDIHLDGSTGGLGFFRREKTGHKRRCHTQCCHSARAAGRECQETAAAW 70 80 90 100 110 120 130 140 150 159 m594.pep DFLIIHYSWLIFWEYRAIKRCΝFTQFAVILKALFKIRX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a594 DFLIIHYSWLIFWEYRAIKRCΝFTQFAVILKALFKIRX 130 140 150 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 1865>: g595. seq.. 1 atgagaaaat tcaatttgac cgcattgtcc gtgatgcttg ccttgggttt 51 gaccgcgtgc cagccgccgg aggcggagaa agccgcgccg gccgcgtccg 101 gtgagaccca atccgccaac gaaggcggtt cggtcggtat cgccgtcaac 151 gacaatgcct gcgaaccgat gaatctgacc gtgccgagcg gacaggttgt 201 gttcaatatt aaaaacaaca gcggccgcaa gctcgaatgg gaaatcctga 251 agggcgtgat ggtggtggac gaacgcgaaa atatcgcccc ggggctttcc 301 gacaaaatga accgtaacct gctgccgggc gaatacgaaa tgacctgcgg 351 ccttttgacc aatccgcgcg gcaagctggt ggtagccgac agcggcttta 401 aagacaccgc caacgaagcg gatttggaaa aactgcccca accgctcgcc 451 gactataaag cctacgttca aggcgaggtt aaagagctgg cggcgaaaac 501 caaaaccttt accgaagccg tcaaagcagg cgacattgaa aaggcgaaat 551 ccctgtttgc cgccacccgc gtccattacg aacgcatcga accgattgcc 601 gagcttttca gcgaactcga ccccgtcatc gatgcgtgtg aagacgactt 651 caaagacggt gcgaaagatg ccgggtttac cggcttccac cgtatcgaac 701 acgccctttg ggtggaaaaa gacgtatccg gcgtgaagga aaccgcggcc 751 aaactgatga ccgatgtcga agccctgcaa aaagaaatcg acgcattggc 801 gttccctccg ggcaaagtgg tcggcggcgc gtccgaactg attgaagaag 851 cggcgggcag taaaatcagc ggcgaagaag accgttacag ccacaccgat 901 ttgagcgact tccaagctaa tgcggacgga tctaaaaaaa tcgtcgattt 951 gttccgtccg ttgattgagg ccaaaaacaa agccttgttg gaaaaaaccg 001 ataccaactt caaacaggtc aacgaaattc tggcgaaata ccgcaccaaa 1051 gacggttttg aaacctacga caagctgagc gaagccgacc gcaaagcatt 1101 acaggctcct attaacgcgc ttgccgaaga ccttgcccaa cttcgcggca 1151 tactcggctt gaaataa This corresponds to the amino acid sequence <SEQ ID 1866; ORF 595.ng>: g595.pep 1 MRKFNLTALS VMLALGLTAC QPPEAEKAAP AASGETQSAN EGGSVGIAVN 51 DNACEPMNLT VPSGQWFNI KNNSGRKLEW EILKGVMWD ERENIAPGLS 101 DKMNRNLLPG EYEMTCGLLT NPRGKLWAD SGFKDTANEA DLEKLPQPLA 151 DYKAYVQGEV KELAAKTKTF TEAVKAGDIE KAKSLFAATR VHYERIEPIA 201 ELFSELDPVI DACEDDFKDG AKDAGFTGFH RIEHALWVEK DVSGVKETAA 251 KLMTDVEALQ KEIDALAFPP GKWGGASEL IEEAAGSKIS GEEDRYSHTD 301 LSDFQANADG SKKIVDLFRP LIEAKNKALL EKTDTNFKQV NEILAKYRTK 351 DGFETYDKLS EADRKALQAP INALAEDLAQ LRGILGLK* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1867>: m595. seq i ATGAGAAAAT TCAATTTGAC CGCATTGTCC GTGATGCTTG CCTTAGGTTT 51 GACCGCGTGC CAGCCGCCGG AGGCGGAGAA AGCTGCGCCG GCAGCGTCCG 101 GTGAGGCGCA AACCGCCAAC GAGGGCGGTT CGGTCAGTAT CGCCGTCAAC 151 GACAATGCCT GCGAACCGAT GGAACTGACC GTGCCGAGCG GACAGGTTGT 201 GTTCAATATT AAAAACAACA GCGGCCGCAA GCTCGAATGG GAAATCCTGA 251 AAGGCGTGAT GGTGGTGGAC GAGCGCGAAA ACATCGCCCC CGGACTTTCC 301 GATAAAATGA CCGTCACCCT GTTGCCGGGC GAATACGAAA TGACTTGCGG 351 TCTTTTGACC AATCCGCGCG GCAAGCTGGT GGTAACCGAC AGCGGCTTTA 401 AAGACACCGC CAACGAAGCG GATTTGGAAA AACTGTCCCA ACCGCTCGCC 451 GACTATAAAG CCTACGTTCA AGGCGAGGTT AAAGAGCTGG TGGCGAAAAC 501 CAAAACTTTT ACCGAAGCCG TCAAAGCAGG CGACATTGAA AAGGCGAAAT 551 CCCTGTTTGC CGACACCCGC GTCCATTACG AACGCATCGA ACCGATTGCC 601 GAGCTTTTCA GCGAACTCGA CCCCGTCATC GATGCGCGTG AAGACGACTT 651 CAAAGACGGC GCGAAAGATG CCGGATTTAC CGGCTTTCAC CGTATCGAAT 701 ACGCCCTTTG GGTGGAAAAA GACGTGTCCG GCGTGAAGGA AATTGCAGCG 751 AAACTGATGA CCGATGTCGA AGCCCTGCAA AAAGAAATCG ACGCATTGGC 801 GTTTCCTCCG GGCAAGGTGG TCGGCGGCGC GTCCGAACTG ATTGAAGAAG 851 TGGCGGGCAG TAAAATCAGC GGCGAAGAAG ACCGGTACAG CCACACCGAT 901 TTGAGCGACT TCCAAGCCAA TGTGGACGGA TCTAAAAAAA TCGTCGATTT 951 GTTCCGTCCG CTGATCGAGG CCAAAAACAA AGCCTTGTTG GAAAAAACCG 1001 ATACCAACTT CAAACAGGTC AACGAAATTC TGGCGAAATA CCGGACTAAA 1051 GACGGTTTTG AAACCTACGA CAAGCTGGGC GAAGCCGACC GCAAAGCGTT 1101 ACAGGCCTCT ATTAACGCGC TTGCCGAAGA CCTTGCCCAA CTTCGCGGCA 1151 TACTCGGCTT GAAATAA This corresponds to the amino acid sequence <SEQ ID 1868; ORF 595>: m.595.pep 1 MRKFNLTALS VMLALGLTAC QPPEAEKAAP AASGEAQTAN EGGSVSIAVN 51 DNACEPMELT VPSGQWFNI KNNSGRKLEW EILKGVMWD ERENIAPGLS 101 DKMTVTLLPG EYEMTCGLLT NPRGKLWTD SGFKDTANEA DLEKLSQPLA 151 DYKAYVQGEV KELVAKTKTF TEAVKAGDIE KAKSLFADTR VHYERIEPIA 201 ELFSELDPVI DAREDDFKDG AKDAGFTGFH RIEYALWVEK DVSGVKEIAA 251 KLMTDVEALQ KEIDALAFPP GKWGGASEL IEEVAGSKIS GEEDRYSHTD 301 LSDFQANVDG SKKIVDLFRP LIEAKNKALL EKTDTNFKQV NEILAKYRTK 351 DGFETYDKLG EADRKALQAS INALAEDLAQ LRGILGLK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m595 / g595 95.4% identity in 388 aa overlap 10 20 30 40 50 60 m595.pep MRKFΝLTALSVMLALGLTACQPPEAEKAAPAASGEAQTAΝEGGSVSIAVΝDΝACEPMELT II I II II II II II II II II I II II I II I II I II I I : I : II II I II : II II I I I II II : II g595 MRKFΝLTALSVMLALGLTAC Q PPEAEKAAPAASGETQSAΝEGGSVGIAVΝDΝACEPMΝLT 10 20 30 40 50 60 70 30 JO 100 110 120 m595.pep VPSGQWFNIKNNSGRKLEWEILKGVMWDERENIAPGLSDKMTVTLLPGEYEMTCGLLT I I I I I I II II II I II II II II II II I II II II II I II II II II : MMMMIMMM g595 VPSGQWFNIKNNSGRKLEWEILKGVMWDERENIAPGLSDKMNRNLLPGEYEMTCGLLT 70 80 90 100 110 120 130 140 150 160 170 180 m595. pep NPRGKLWTDSGFKDTANEADLEKLSQPLADYKAYVQGEVKELVAKTKTFTEAVKAGDIE I II I I I II : II II II II II II II II II II II II I I II II II I : II II II I I I II I I I I I g595 NPRGKLWADSGFKDTANEADLEKLPQPLADYKAYVQGEVKELAAKTKTFTEAVKAGDIE 130 140 150 160 170 180 190 200 210 220 230 240 m595.pep KAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFKDGAKDAGFTGFHRIEYALWVEK lllllll II I I II I II II II II II I II II II II II II I II II II II II II I : II I II I g595 KAKSLFAATRVHYERIEPIAELFSELDPVIDACEDDFKDGAKDAGFTGFHRIEHALWVEK 190 200 210 220 230 240 250 260 270 280 290 300 m595. pep DVSGVKEIAAKLMTDVEALQKEIDALAFPPGKWGGASELIEEVAGSKISGEEDRYSHTD lllllll I II II I I I II II II I II II II II II I I II II II II : I I I I I II I I II I I II I g595 DVSGVKETAAKLMTDVEALQKEIDALAFPPGKWGGASELIEEAAGSKISGEEDRYSHTD 250 260 270 280 290 300 310 320 330 340 350 360 m595. pep LSDFQANVDGSKKIVDLFRPLIEAKNKALLEKTDTNFKQVNEILAKYRTKDGFETYDKLG II I II I I M II II II II II II II II II II II I II I I II II II II I II II I I II II II II : g595 LSDFQANADGSKKIVDLFRPLIEAKNKALLEKTDTNFKQVNEILAKYRTKDGFETYDKLS 310 320 330 340 350 360 370 380 389 m595. pep EADRKALQASINALAEDLAQLRGILGLKX lllllllll I II I I II II I II II II II I g595 EADRKALQAPINALAEDLAQLRGILGLKX 370 380 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1869>: a595. seq 1 ATGAGAAAAT TCAATTTGAC CGCATTGTCC GTGATGCTTG CCTTAGGTTT 51 GACCGCGTGC CAGCCGCCGG AGGCGGAGAA AGCTGCGCCG GCAGCGTCCG 101 GTGAGGCGCA AACCGCCAAC GAGGGCGGTT CGGTCAGTAT CGCCGTCAAC 151 GACAATGCCT GCGAACCGAT GGAACTGACC GTGCCGAGCG GACAGGTTGT 201 GTTCAATATT AAAAACAACA GCGGCCGCAA GCTCGAATGG GAAATCCTGA 251 AAGGCGTGAT GGTGGTGGAC GAGCGCGAAA ACATCGCCCC CGGACTTTCC 301 GATAAAATGA CCGTCACCCT GTTGCCGGGC GAATACGAAA TGACTTGCGG 351 TCTTTTGACC AATCCGCGCG GCAAGCTGGT GGTAACCGAC AGCGGCTTTA 401 AAGACACCGC CAACGAAGCG GATTTGGAAA AACTGTCCCA ACCGCTCGCC 451 GACTATAAAG CCTATGTTCA AGGCGAAGTC AAAGAGCTGG TGGCGAAAAC 501 CAAAACCTTT ACCGAAGCCG TCAAAGCAGG CGACATTGAA AAGGCGAAAT 551 CCCTGTTTGC CGACACCCGC GTCCATTACG AACGCATCGA ACCGATTGCC 601 GAGCTTTTCA GCGAACTCGA CCCCGTCATC GATGCGCGTG AAGACGACTT 651 CAAAGACGGC GCGAAAGATG CCGGATTTAC CGGCTTCCAC CGTATCGAAT 701 ACGCCCTTTG GGTGGAAAAA GACGTGTCCG GCGTGAAGGA AATTGCAGCG 751 AAACTGATGA CCGATGTCGA AGCCCTGCAA AAAGAAATCG ACGCATTGGC 801 GTTTCCTCCG GGCAAGGTGG TCGGCGGCGC GTCCGAACTG ATTGAAGAAG 851 TGGCGGGCAG TAAAATCAGC GGCGAAGAAG ACCGGTACAG CCACACCGAT 901 TTGAGCGACT TCCAAGCCAA TGTGGACGGA TCGAAAAAAA TCGTCGATTT 951 GTTCCGTCCG TTGATCGAGA CCAAAAACAA AGCCTTGTTG GAAAAAACCG 1001 ATACCAACTT CAAACAGGTC AACGAAATTC TGGCGAAATA CCGGACTAAA 1051 GACGGTTTTG AAACCTACGA CAAGCTGGGC GAAGCCGACC GCAAAGCGTT 1101 ACAGGCCTCT ATTAACGCGC TTGCCGAAGA CCTTGCCCAA CTTCGCGGCA 1151 TACTCGGCTT GAAATAA This corresponds to the amino acid sequence <SEQ ID 1870; ORF 595. a>: a595.pep 1 MRKFNLTALS VMLALGLTAC QPPEAEKAAP AASGEAQTAN EGGSVSIAVN 51 DNACEPMELT VPSGQWFNI KNNSGRKLEW EILKGVMWD ERENIAPGLS 101 DKMTVTLLPG EYEMTCGLLT NPRGKLWTD SGFKDTANEA DLEKLSQPLA 151 DYKAYVQGEV KELVAKTKTF TEAVKAGDIE KAKSLFADTR VHYERIEPIA 201 ELFSELDPVI DAREDDFKDG AKDAGFTGFH RIEYALWVEK DVSGVKEIAA 251 KLMTDVEALQ KEIDALAFPP GKWGGASEL IEEVAGSKIS GEEDRYSHTD 301 LSDFQANVDG SKKIVDLFRP LIETKNKALL EKTDTNFKQV NEILAKYRTK 351 DGFETYDKLG EADRKALQAS INALAEDLAQ LRGILGLK* m595/a595 99.7% identity in 388 aa overlap 10 20 30 40 50 60 m595. pep MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGSVSIAVNDNACEPMELT MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a595 MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGSVSIAVNDNACEPMELT 10 20 30 40 50 60 70 80 90 100 110 120 m595.pep VPSGQWFNIKNNSGRKLEWEILKGVMWDERENIAPGLSDKMTVTLLPGEYEMTCGLLT II I I II II II I I II I II II I II II II II II II I II II II I I II I II II II I I I I I M I I I a595 VPSGQWFNIKNNSGRKLEWEILKGVMWDERENIAPGLSDKMTVTLLPGEYEMTCGLLT 70 80 90 100 110 120 130 140 150 160 170 180 m595. pep NPRGKLWTDSGFKDTANEADLEKLSQPLADYKAYVQGEVKELVAKTKTFTEAVKAGDIE II II II I I II II II II II I II II II I II II II I II II I II I II II II II II I I II II I I I a595 NPRGKLWTDSGFKDTANEADLEKLSQPLADYKAYVQGEVKELVAKTKTFTEAVKAGDIE 130 140 150 160 170 180 190 200 210 220 230 240 m595.pep KAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFKDGAKDAGFTGFHRIEYALWVEK II I II II I II I I II II II II II I II II II II II II II II II I II I II II II II II I II I I a595 KAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFKDGAKDAGFTGFHRIEYALWVEK 190 200 210 220 230 240 250 260 270 280 290 300 m595.pep DVSGVKEIAAKLMTDVEALQKEIDALAFPPGKWGGASELIEEVAGSKISGEEDRYSHTD II II I II I I I II I II II II I II II II II II II I II II II II II II II II I II II II II I I a595 DVSGVKEIAAKLMTDVEALQKEIDALAFPPGKWGGASELIEEVAGSKISGEEDRYSHTD 250 260 270 280 290 300 310 320 330 340 350 360 m595. pep LSDFQANVDGSKKIVDLFRPLIEAKNKALLEKTDTNFKQVNEILAKYRTKDGFETYDKLG MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a595 LSDFQANVDGSKKIVDLFRPLIETKNKALLEKTDTNFKQVNEILAKYRTKDGFETYDKLG 310 320 330 340 350 360 370 380 389 m595 . pep EADRKALQASINALAEDLAQLRGILGLKX M M M M M M M M M M M I M M M a595 EADRKALQASINALAEDLAQLRGILGLKX 370 380 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1871>: g596.seq. (partial) . 1 ..atgctgctct tggacgagcc gaccaaccac ttggatgcgg aatcggtgga 51 atggctggag caattcctcg tgcgcttccc cggcacagtg gtcgcggtaa 101 cgcacgaccg ctacttcctc gacaacgccg ccgaatggat tttggaactc 151 gaccgcggac acggcattcc gtggaaaggc aattactcgt cttggctgga 201 gcagaaagaa aaacgcttgg aaaacgaggc gaaatccgaa gccgcgcgcg 251 tgaaggcgat gaagcaggaa ttggaatggg tgcgccaaaa tgccaaaggc 301 cgccaagcca agcccaaagc gcgtttggcg cgttttgaag aaatgagcaa 351 ctacgaatac caaaaacgca acgaaactca ggaaatcttt atccctgttg 401 ccgagcgttt gggtaacgaa gtgattgaat ttgtgaatgt ttccaaatcg 451 ttcggcgata aagtgctgat tgacggtttg agcttcaaag tgccggcggg 501 cgcgattgtc ggcatcatcg gcccgaacgg cgcgggtaaa tcgacgctgt 551 tcaaaatgat tgcgggcaaa gagcagcccg attcgggcga agtgaaaatc 601 gggcaaaccg tgaaaatgag cttgattgac caaagccgcg aaggtttgca 651 aaacgacaaa accgtgttcg acaacattgc cgaaggtcgc gatattttgc 701 aggtcggaca gtttgaaatc cccgcccgcc aatatttggg acgcttcaac 751 tttaaaggca gcgaccaaag caaaatcgca aggcagcttt ccggcggcga 801 acgcggccgt ctgcacttgg caaaaacctt gttgggcggc ggcaatgtgt 851 tgctgctgga cgaaccgtcc aacgatctcg acgtggaaac cctgcgcgcg 901 ttggaagacg cattgttgga atttgccggc agcgtgatgg tgatttcgca 951 cgaccgctgg tttctcgacc gcatagccac gcatatcttg gcgtgtgaag 1001 gcgactccaa atgggtgttc ttcgacggca actatcaaga atacgaagcc 1051 gacaagaaac gccgactcgg caaagaaggc gcgaaaccga aacgcatcaa 1101 atacaaaccg gtaacgcgtt aa This corresponds to the amino acid sequence <SEQ ID 1872; ORF 596.ng>: g596.pep (partial) . 1 ..MLLLDEPTNH LDAESVEWLE QFLVRFPGTV VAVTHDRYFL DNAAEWILEL 51 DRGHGIPWKG NYSSWLEQKE KRLENEAKSE AARVKAMKQE LEWVRQNAKG 101 RQAKPKARLA RFEEMSNYEY QKRNETQEIF IPVAERLGNE VIEFVNVSKS 151 FGDKVLIDGL SFKVPAGAIV GIIGPNGAGK STLFKMIAGK EQPDSGEVKI 201 GQTVKMSLID QSREGLQNDK TVFDNIAEGR DILQVGQFEI PARQYLGRFN 251 FKGSDQSKIA RQLSGGERGR LHLAKTLLGG GNVLLLDEPS NDLDVETLRA 301 LEDALLEFAG SVMVISHDRW FLDRIATHIL ACEGDSKWVF FDGNYQEYEA 351 DKKRRLGKEG AKPKRIKYKP VTR* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1873>: m596. seq.. 1 ATGTCCCAAC AATACGTCTA TTCTATGCTG CGCGTGAGCA AGGTTGTGCC 51 GCCGCAGAAA ACCATCATTA AAGATATTTC CCTTTCTTTC TTCCCCGGCG 101 CGAAAATCGG CCTGCTCGGT TTGAACGGCG CGGGCAAGTC CACCGTGCTG 151 CGGATTATGG CGGGCGTGGA TAAGGAATTT GAGGGCGAAG CCGTGCCGAT 201 GGGCGGCATC AAAATCGGCT ACCTGCCGCA AGAGCCTGAG CTTGATCCGG 251 AAAAAACCGT GCGCGAGGAA GTGGAAAGCG GTTTGGGCGA AGTGGCTGCC 301 GCGCAGAAAC GTTTGGAAGA AGTGTATGCC GAGTACGCCA ATCCTGATGC 351 GGATTTTGAC GCGTTGGCAG AAGAGCAGGG CCGCTTGGAA GCGATTATTG 401 CGGCAGGTTC GTCCACGGGC GGCGGTGCGG AACACGAATT GGAAATCGCC 451 GCCGACGCGC TGCGCCTGCC GGAATGGGAT GCCAAAATCG ATAATTTGTC 501 CGGCGGTGAA AAACGCCGCG TTGCCTTGTG CAAACTCTTG TTGAGCAAGC 551 CCGATATGCT TTTGCTGGAC GAGCCGACCA ACCACTTGGA TGCGGAATCG 601 GTCGAGTGGC TGGAGCAATT TCTCGTGCGC TTCCCCGGCA CAGTCGTTGC 651 GGTAACGCAC GACCGCTACT TCCTCGACAA CGCCGCCGAA TGGATTTTGG 701 AACTCGACCG CGGCCATGGT ATTCCGTGGA AAGGCAATTA CTCGTCTTGG 751 CTGGAGCAGA AAGAAAAACG CTTGGAAAAC GAGGCAAAAT CCGAAGCCGC 801 GCGCGTGAAG GCGATGAAGC AGGAATTGGA ATGGGTGCGC CAAAATGCCA 851 AAGGCCGCCA AGCCAAGTCC AAAGCGCGTT TGGCTCGTTT TGAAGAAATG 901 AGCAACTACG AATACCAAAA ACGCAATGAA ACGCAGGAAA TCTTTATTCC 951 CGTTGCCGAG CGTTTGGGTA ACGAAGTGAT TGAATTTGTA AATGTTTCCA 1001 AATCGTTCGG CGATAAAGTG CTGATTGACG ATTTGAGCTT CAAAGTGCCT 1051 GCGGGCGCGA TTGTCGGCAT CATCGGCCCG AACGGCGCGG GTAAATCTAC 1101 GCTGTTCAAA ATGATTTCGG GCAAAGAGCA GCCTGATTCC GGCGAGGTGA 1151 AAATCGGACA AACCGTGAAA ATGAGCTTGA TTGACCAAAG CCGCGAAGGT 1201 TTGCAAAACG ACAAAACCGT GTTCGACAAC ATTGCCGAAG GCCGCGACAT 1251 TTTGCAGGTT GGTCAGTTTG AAATTCCCGC CCGCCAATAT TTGGGGCGTT 1301 TCAACTTCAA AGGCAGCGAC CAAAGCAAAA TTGCAGGTCA ATTGTCTGGC 1351 GGCGAACGCG GTCGTCTGCA CTTGGCAAAA ACCTTGTTGA GCGGCGGCAA 1401 TGTATTGCTG CTGGATGAAC CGTCTAACGA CCTTGACGTG GAAACCCTGC 1451 GCGCGTTGGA AGACGCATTG TTGGAATTTG CCGGCAGCGT GATGGTGATT 1501 TCGCACGACC GTTGGTTCCT CGACCGCATC GCCACGCATA TCTTGGCGTG 1551 TGAAGGCGAC TCTAAATGGG TGTTCTTCGA CGGCAACTAT CAGGAATACG 1601 AAGCCGACAA GAAACGCCGT TTGGGCGAAG AAGGCGCGAA ACCGAAACGC 1651 ATCAAATACA AACCGGTAAC GCGTTAA This corresponds to the amino acid sequence <SEQ ID 1874; ORF 596>: m596. pep . . 1 MSQQYVYSML RVSKWPPQK TIIKDISLSF FPGAKIGLLG LNGAGKSTVL 51 RIMAGVDKEF EGEAVPMGGI KIGYLPQEPE LDPEKTVREE VESGLGEVAA 101 AQKRLEEVYA EYANPDADFD ALAEEQGRLE AIIAAGSSTG GGAEHELEIA 151 ADALRLPEWD AKIDNLSGGE KRRVALCKLL LSKPDMLLLD EPTNHLDAES 201 VEWLEQFLVR FPGTVVAVTH DRYFLDNAAE WILELDRGHG IPWKGNYSSW 251 LEQKEKRLEN EAKSEAARVK AMKQELEWVR QNAKGRQAKS KARLARFEEM 301 SNYEYQKRNE TQEIFIPVAE RLGNEVIEFV NVSKSFGDKV LIDDLSFKVP 351 AGAIVGIIGP NGAGKSTLFK MISGKEQPDS GEVKIGQTVK MSLIDQSREG 401 LQNDKTVFDN IAEGRDILQV GQFEIPARQY LGRFNFKGSD QSKIAGQLSG 451 GERGRLHLAK TLLSGGNVLL LDEPSNDLDV ETLRALEDAL LEFAGSVMVI 501 SHDRWFLDRI ATHILACEGD SKWVFFDGNY QEYEADKKRR LGEEGAKPKR 551 IKYKPVTR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m596 g596 98.4% identity in 373 aa overlap 160 170 180 190 200 210 m596.pep LPEWDAKIDΝLSGGEKRRVALCKLLLSKPDMLLLDEPTΝHLDAESVEWLEQFLVRFPGTV II II II I II II I II II II II II II II I II I g596 MLLLDEPTΝHLDAESVEWLEQFLVRFPGTV 10 20 30 220 230 240 250 260 270 m596. pep VAVTHDRYFLDΝAAEWILELDRGHGIPWKGΝYSSWLEQKEKRLEΝEAKSEAARVKAMKQE I II I II II II II I II II II II II II II I II II II I II II I II II I II I II II II I II II I g596 VAVTHDRYFLDΝAAEWILELDRGHGIPWKGΝYSSWLEQKEKRLEΝEAKSEAARVKAMKQE 40 50 60 70 80 90 280 290 300 310 320 330 m596.pep LEWVRQΝAKGRQAKSKARLARFEEMSΝYEYQKRΝETQEIFIPVAERLGΝEVIEFVΝVSKS llllllllllllll II II II II II I II I II II II II II I II II II II II II II I II II I g596 LEWVRQΝAKGRQAKPKARLARFEEMSΝYEYQKRΝETQEIFIPVAERLGΝEVIEFVΝVSKS 100 110 120 130 140 150 340 350 360 370 380 390 m596.pep FGDKVLIDDLSFKVPAGAIVGIIGPΝGAGKSTLFKMISGKEQPDSGEVKIGQTVKMSLID llllllll I II II II II II II II I II I II II II II I : II II II II II II I II II I II I I g596 FGDKVLIDGLSFKVPAGAIVGIIGPΝGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMSLID 160 170 180 190 200 210 400 410 420 430 440 450 m596.pep QSREGLQΝDKTVFDΝIAEGRDILQVGQFEIPARQYLGRFΝFKGSDQSKIAGQLSGGERGR II II I II II II II II I I II I II I I II II I II II I I II II I II I II I I I II lllllllll g596 QSREGLQΝDKTVFDΝIAEGRDILQVGQFEIPARQYLGRFΝFKGSDQSKIARQLSGGERGR 220 230 240 250 260 270 460 470 480 490 500 510 m596.pep LHLAKTLLSGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLDRIATHIL II I II I I I : II II I II II II II I II II I I II II I II II II II II I I II II II II I II II I g596 LHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLDRIATHIL 280 290 300 310 320 330 520 530 540 550 559 m596. pep ACEGDSKWVFFDGNYQEYEADKKRRLGEEGAKPKRIKYKPVTRX I I II II I II II II I II II I II II II II : I II II I II II II II II g596 ACEGDSKWVFFDGNYQEYEADKKRRLGKEGAKPKRIKYKPVTRX 340 350 360 370 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1875>: a596. seq 1 ATGTCCCAAC AATACGTCTA TTCTATGCTG CGCGTGAGCA AGGTTGTGCC 51 GCCGCAGAAA ACCATCATTA AAGATATTTC CCTTTCTTTC TTCCCCGGCG 101 CGAAAATCGG TTTGCTCGGT TTGAACGGCG CGGGCAAGTC CACCGTGCTG 151 CGGATTATGG CGGGCGTGGA TAAAGAATTT GAGGGCGAAG CCGTGCCGAT 201 GGGCGGTATT AAAATCGGCT ACCTGCCGCA AGAGCCTGAG CTTGATCCGG 251 AAAAAACCGT GCGTGAGGAA GTGGAAAGCG GTTTGGGCGA AGTGGCTGCC 301 GCGCAGAAAC GTTTGGAGGA AGTGTATGCC GAGTACGCCA ATCCCGATGC 351 GGATTTTGAC GCGTTGGCGG AAGAGCAGGG GCGTTTGGAA GCGATTATTG 401 CGGCGGGTTC GTCCACGGGC GGCGGTGCGG AACACGAATT GGAAATCGCT 451 GCCGACGCGC TGCGCCTGCC GGAATGGGAT GCCAAAATCG ATAATTTGTC 501 CGGCGGTGAA AAACGCCGCG TCGCTTTGTG CAAACTCTTG TTGAGCAAGC 551 CCGATATGCT TTTGCTGGAC GAGCCGACCA ACCACTTGGA TGCGGAATCG 601 GTCGAGTGGC TGGAGCAATT TCTCGTGCGC TTCCCCGGTA CAGTCGTTGC 651 CGTAACACAC GACCGCTACT TCCTCGACAA CGCCGCCGAA TGGATTTTGG 701 AACTCGACCG CGGGCACGGT ATTCCGTGGA AAGGAAATTA CTCGTCTTGG 751 TTGGAGCAGA AAGAAAAACG TTTGGAAAAC GAGGCGAAAT CCGAAGCCGC 801 GCGCGTGAAA GCGATGAAGC AGGAATTGGA ATGGGTGCGC CAAAATGCCA 851 AAGGCCGTCA AGCCAAGTCC AAAGCGCGTT TGGCGCGTTT TGAAGAAATG 901 AGCAACTATG AATACCAAAA ACGCAATGAA ACGCAGGAAA TCTTCATTCC 951 CGTCGCCGAG CGTTTGGGTA ACGAAGTGAT TGAATTTGTG AATGTTTCCA 1001 AATCGTTCGG CGACAAAGTG CTGATTGACG ATTTGAGCTT CAAAGTGCCT 1051 GCGGGCGCGA TTGTCGGCAT CATCGGTCCG AACGGCGCGG GTAAATCGAC 1101 ACTGTTTAAA ATGATTGCGG GCAAAGAGCA GCCCGATTCC GGTGAAGTGA 1151 AAATCGGGCA AACCGTGAAA ATGAGCTTGA TTGACCAAAG CCGCGAAGGT 1201 TTGCAAAACG ACAAAACCGT GTTCGACAAC ATTGCCGAAG GTCGCGATAT 1251 TTTACAGGTC GGGCAGTTTG AAATCCCCGC CCGCCAATAT TTGGGACGCT 1301 TCAATTTCAA AGGCAGCGAC CAAAGCAAAA TCACGGGGCA GCTTTCCGGC 1351 GGCGAACGCG GACGTTTGCA CTTGGCAAAA ACCTTGTTGG GCGGTGGCAA 1401 TGTGTTGCTG CTGGACGAAC CGTCCAACGA CCTCGACGTG GAAACCCTGC 1451 GCGCGTTGGA AGACGCATTG CTGGAATTTG CCGGCAGCGT GATGGTGATT 1501 TCGCACGACC GCTGGTTCCT CGACCGTATT GCTACGCATA TCTTGGCTTG 1551 CGAAGGCGAC TCCAAATGGG TGTTCTTTGA CGGCAACTAT CAGGAATACG 1601 AAGCCGACAA GAAACGCCGA CTCGGCGAAG AAGGCACGAA ACCGAAACGC 1651 ATCAAATACA AACCGGTAAC GCGTTAA This corresponds to the amino acid sequence <SEQ LD 1876; ORF 596. a>: a596.pep 1 MSQQYVYSML RVSKWPPQK TIIKDISLSF FPGAKIGLLG LNGAGKSTVL 51 RIMAGVDKEF EGEAVPMGGI KIGYLPQEPE LDPEKTVREE VESGLGEVAA 101 AQKRLEEVYA EYANPDADFD ALAEEQGRLE AIIAAGSSTG GGAEHELEIA 151 ADALRLPEWD AKIDNLSGGE KRRVALCKLL LSKPDMLLLD EPTNHLDAES 201 VEWLEQFLVR FPGTVVAVTH DRYFLDNAAE WILELDRGHG IPWKGNYSSW 251 LEQKEKRLEN EAKSEAARVK AMKQELEWVR QNAKGRQAKS KARLARFEEM 301 SNYEYQKRNE TQEIFIPVAE RLGNEVIEFV NVSKSFGDKV LIDDLSFKVP 351 AGAIVGIIGP NGAGKSTLFK MIAGKEQPDS GEVKIGQTVK MSLIDQSREG 401 LQNDKTVFDN IAEGRDILQV GQFEIPARQY LGRFNFKGSD QSKITGQLSG 451 GERGRLHLAK TLLGGGNVLL LDEPSNDLDV ETLRALEDAL LEFAGSVMVI 501 SHDRWFLDRI ATHILACEGD SKWVFFDGNY QEYEADKKRR LGEEGTKPKR 551 IKYKPVTR* m596/a596 99.3% identity in 558 aa overlap 10 20 30 40 50 60 m596.pep MSQQYVYSMLRVSKWPPQKTIIKDISLSFFPGAKIGLLGLNGAGKSTVLRIMAGVDKEF II II II II II II II I II II I II I II I II II I II II I I II II II II II II II II I II II I I a596 MSQQYVYSMLRVSKWPPQKTIIKDISLSFFPGAKIGLLGLNGAGKSTVLRIMAGVDKEF 10 20 30 40 50 60 70 80 90 100 110 120 m596. pep EGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQKRLEEVYAEYANPDADFD II II II II II II II I II I II II I II I II II II II II II I II I II I I II II II II II I I I I a596 EGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQKRLEEVYAEYANPDADFD 70 80 90 100 110 120 130 140 150 160 170 180 m596. pep ALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWDAKIDNLSGGEKRRVALCKLL I II II I II II II II II II II II I II I II II II II II II I II II I II I I I I II II I II I I I a596 ALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWDAKIDNLSGGEKRRVALCKLL 130 140 150 160 170 180 190 200 210 220 230 240 m596. pep LSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTWAVTHDRYFLDNAAEWILELDRGHG II I II I II II II II I II I II II I II I II I II I I I II II II I I II I I II II II I I I I I I I I a596 LSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTWAVTHDRYFLDNAAEWILELDRGHG 190 200 210 220 230 240 250 260 270 280 290 300 m596. pep IPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQELEWVRQNAKGRQAKSKARLARFEEM II II II II II I II II II II II II II I II I I II II II II II II I II II II II II I II II I I a596 IPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQELEWVRQNAKGRQAKSKARLARFEEM 250 260 270 280 290 300 310 320 330 340 350 360 m596.pep SNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSKSFGDKVLIDDLSFKVPAGAIVGIIGP II II II II II I II II II II II II II II II II II I II II I II II I I I I II II II I II II II a596 SNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSKSFGDKVLIDDLSFKVPAGAIVGIIGP 310 320 330 340 350 360 370 380 390 400 410 420 m596.pep NGAGKSTLFKMISGKEQPDSGEVKIGQTVKMSLIDQSREGLQNDKTVFDNIAEGRDILQV MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a596 NGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMSLIDQSREGLQNDKTVFDNIAEGRDILQV 370 380 390 400 410 420 430 440 450 460 470 480 m596.pep GQFEIPARQYLGRFNFKGSDQSKIAGQLSGGERGRLHLAKTLLSGGNVLLLDEPSNDLDV MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a596 GQFEIPARQYLGRFNFKGSDQSKITGQLSGGERGRLHLAKTLLGGGNVLLLDEPSNDLDV 430 440 450 460 470 480 490 500 510 520 530 540 m596.pep ETLRALEDALLEFAGSVMVISHDRWFLDRIATHILACEGDSKWVFFDGNYQEYEADKKRR II II II II II II II II II M II II II II II II I II II II I I II I I II I I I I I I I II II II a596 ETLRALEDALLEFAGSVMVISHDRWFLDRIATHILACEGDSKWVFFDGNYQEYEADKKRR 490 500 510 520 530 540 550 559 m596.pep LGEEGAKPKRIKYKPVTRX II I II : II II II II II II I a596 LGEEGTKPKRIKYKPVTRX 550 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1877> g597. seq 1 ATGCTGCTTC ATGTCAGCAA TTCCCTCAAA CAGCTTCAGG AAGAGCGTAT 51 CCGCCAAGAA CGTATCCGCC AAGAGCGTAT CCGTCAGGCG CGCGGCAACC 101 TTGCTTCCGT CAACCGCAAA CAGCGCGAGG CTTGGGACAA ATTCCAAAAA 151 CTCAATACCG AGCTGAACCG TTTGAAAACG GAAGTCGCCG CTACGAAAGC 201 GCAGATTTCC CGTTTCGTAT CGGGGAACTA TAAAAACAGC CGGCCGAATG 251 CGGTTGCCCT GTTCCTGAAA AACGCCGAAC CGGGTCAGAA AAACCGCTTT 301 TTGCGTTATA CGCGTTATGT AAACGCCTCC AATCGGGAAG TTGTCAAGGA 351 TTTGGAAAAA CAGCAGAAGG CTTTGGCGGT ACAAGAGCAG AAAATCAACA 401 ATGAGCTTGC CCGTTTGAAG AAAATTCAGG CAAACGTGCA ATCCCTGCTG 451 AAAAAACAGG GTGTAACCGA TGCGGCGGAA CAGACGGAAA GCCGCAGACA 501 GAATGCCAAA ATCTCCAAAG ATGCCCGAAA ACTGCTGGAA CAGAAAGGGA 551 ACGAGCAGCA GCTGAACAAG CTCTTGAGCA ATTTGgagaa aaAAAaagcc 601 gaacaccgCA TTcaggAtgc ggAagcaaAA agaAAATTGG CTGAagcCaa 651 actGgcggca gccgAAAAAG CCAGAAAAGA AGCGGCGCAG CAGAAGGCTG 701 AAGCGCGACG TGCGGAAATG TCCAACCTGA CCGCCGAAGA CAGGAACATC 751 CAAGCGCCTT CGGTTATGGG TATCGGCAGT GCCGACGgTT TCAGCCGCAT 801 GCAGGGACGT TTGAAAAAAC CGGTTGACGG TGTGCCGACC GGGCTTTTCG 851 GGCAGAACCG GAGCGGcggC GATGTTTGGA AAGGCGTGTT CTATTCCACT 901 GCGCCTGCAA CGGTTGAAAG CATTGCGCcg gGAACggtaa GCTATGCGGA 951 cgaGTTGGAC GGCTACGGCA AAGTGGTCGT GATCGATCAC GGCGAGAACT 1001 ACATCAGCAT CTATGCCGGT TTGAGCGAAA TTTCCGCCGG CAAGGGTTAT 1051 ACGGTCGCGG CAGGAAGCAA AATCGGCACG AGCGGGTCGC TGCCGGACGG 1101 GGAAGAGGGG CTTTACCTGC AAATACGTTA TCGAGGTCAG GTGTTGAACC 1151 CTTCGGGCTG GATACGTTGA This corresponds to the amino acid sequence <SEQ ID 1878; ORF 597 >: g597.pep 1 MLLHVSNSLK QLQEERIRQE RIRQERIRQA RGNLASVNRK QREAWDKFQK 51 LNTELNRLKT EVAATKAQIS RFVSGNYKNS RPNAVALFLK NAEPGQKNRF 101 LRYTRYVNAS NREVVKDLEK QQKALAVQEQ KINNELARLK KIQANVQSLL 151 KKQGVTDAAE QTESRRQNAK ISKDARKLLE QKGNEQQLNK LLSNLEKKKA 201 EHRIQDAEAK RKLAEAKLAA AEKARKEAAQ QKAEARRAEM SNLTAEDRNI 251 QAPSVMGIGΞ ADGFΞRMQGR LKKPVDGVPT GLFGQNRSGG DVWKGVFYΞT 301 APATVESIAP GTVSYADELD GYGKVVVIDH GENYISIYAG LSEISAGKGY 351 TVAAGSKIGT SGSLPDGEEG LYLQIRYRGQ VLNPSGWIR* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1879>: m597.seq 1 ATGCTGCTTC ATGTCAGCAA TTCCCTCAAA CAGCTTCAGG AAGAGCGTAT 51 CCGCCAAGAG CGTATCCGTC AGGCGCGCGG CAACCTTGCT TCCGTCAACC 101 GCAAACAGCG CGAGGCTTGG GACAAGTTCC AAAAACTCAA TACCGAGCTG 151 AACCGTTTGA AAACGGAAGT CGCCGCTACG AAAGCGCAGA TTTCCCGTTT 201 CGTATCGGGG AACTATAAAA ACAGCCAGCC GAATGCGGTT GCCCTGTTCC 251 TGAAAAACGC CGAACCGGGT CAGAAAAACC GCTTTTTGCG TTATACGCGT 301 TATGTAAACG CCTCCAATCG GGAAGTTGTC AAGGATTTGG AAAAACAGCA 351 GAAGGCTTTG GCGGTACAAG AGCAGAAAAT CAACAATGAG CTTGCCCGTT 401 TGAAGAAAAT TCAGGCAAAC GTGCAATCTC TGCTGAAAAA ACAGGGTGTA 451 ACCGATGCGG CGGAACAGAC GGAAAGCCGC AGACAGAATG CCAAAATCGC 501 CAAAGATGCC CGAAAACTGC TGGAACAGAA AGGGAACGAG CAGCAGCTGA 551 ACAAGCTCTT GAGCAATTTG GAGAAGAAAA AGGCCGAACA CCGCATTCAG 601 GATGCGGAAG CAAAAAGAAA ATTGGCTGAA GCCAGACTGG CGGCAGCCGA 651 AAAAGCCAGA AAAGAAGCGG CGCAGCAGAA GGCTGAAGCA CGACGTGCGG 701 AAATGTCCAA CCTGACCGCC GAAGACAGGA ACATCCAAGC GCCTTCGGTT 751 ATGGGTATCG GCAGTGCCGA CGGTTTCAGC CGCATGCAAG GACGTTTGAA 801 AAAACCGGTT GACGGTGTGC CGACCGGACT TTTCGGGCAG AACCGGAGCG 851 GCGGCGATAT TTGGAAAGGC GTGTTCTATT CCACTGCACC GGCAACGGTT 901 GAAAGCATTG CGCCGGGAAC GGTAAGCTAT GCGGACGAGT TGGACGGCTA 951 CGGCAAAGTG GTCGTGGTCG ATCACGGCGA GAACTACATC AGCATCTATG 1001 CCGGTTTGAG CGAAATTTCC GTCGGCAAGG GTTATATGGT CGCGGCAGGA 1051 AGCAAAATCG GCTCGAGCGG GTCGCTGCCG GACGGGGAAG AGGGGCTTTA 1101 CCTGCAAATA CGTTATCAAG GTCAGGTATT GAACCCTTCG AGCTGGATAC 1151 GTTGA This corresponds to the amino acid sequence <SEQ ID 1880; ORF 597>: m597. ep 1 MLLHVSNSLK QLQEERIRQE RIRQARGNLA SVNRKQREAW DKFQKLNTEL 51 NRLKTEVAAT KAQISRFVSG NYKNSQPNAV ALFLKNAEPG QKNRFLRYTR 101 YVNASNREVV KDLEKQQKAL AVQEQKINNE LARLKKIQAN VQSLLKKQGV 151 TDAAEQTESR RQNAKIAKDA RKLLEQKGNE QQLNKLLSNL EKKKAEHRIQ 201 DAEAKRKLAE ARLAAAEKAR KEAAQQKAEA RRAEMSNLTA EDRNIQAPSV 251 MGIGSADGFS RMQGRLKKPV DGVPTGLFGQ NRSGGDIWKG VFYSTAPATV 301 ESIAPGTVSY ADELDGYGKV VVVDHGENYI SIYAGLSEIS VGKGYMVAAG 351 SKIGSSGΞLP DGEEGLYLQI RYQGQVLNPS SWIR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 597 shows 96.1% identity over a 389 aa overlap with a predicted ORF (ORF 597) from N gonorrhoeae: m597/g597 96.1% identity in 389 aa overlap 10 20 30 40 50 60 g597.pep MLLHVSΝSLKQLQEERIRQERIRQERIRQARGΝLASVΝRKQREAWDKFQKLΝTELΝRLKT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m597 MLLHVSΝSLKQLQEERIRQERIRQ ARGΝLASVΝRKQREAWDKFQKLΝTELΝRLKT 10 20 30 40 50 70 80 90 100 110 120 g597.pep EVAATKAQISRFVSGΝYKΝSRPΝAVALFLKΝAEPGQKΝRFLRYTRYVΝASΝREVVKDLEK I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m597 EVAATKAQISRFVSGΝYKΝSQPΝAVALFLKΝAEPGQKΝRFLRYTRYVΝAΞΝREVVKDLEK 60 70 80 90 100 110 130 140 150 160 170 180 g597.pep QQKALAVQEQKIΝΝELARLKKIQAΝVQSLLKKQGVTDAAEQTESRRQΝAKISKDARKLLE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I m597 QQKALAVQEQKIΝΝELARLKKIQAΝVQSLLKKQGVTDAAEQTESRRQΝAKIAKDARKLLE 120 130 140 150 160 170 190 200 210 220 230 240 g597.pep QKGΝEQQLΝKLLΞΝLEKKKAEHRIQDAEAKRKLAEAKLAAAEKARKEAAQQKAEARRAEM I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I m597 QKGΝEQQLΝKLLSΝLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRAEM 180 190 200 210 220 230 250 260 270 280 290 300 g597.pep SΝLTAEDRΝIQAPΞVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQΝRSGGDVWKGVFYST I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I l I I I I I I I 597 SΝLTAEDRΝIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQΝRSGGDIWKGVFYST 240 250 260 270 280 290 310 320 330 340 350 360 g597.pep APATVESIAPGTVSYADELDGYGKVVVIDHGEΝYISIYAGLSEISAGKGYTVAAGSKIGT I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I : I I I I m597 APATVEΞIAPGTVSYADELDGYGKVVVVDHGEΝYIΞIYAGLSEISVGKGYMVAAGSKIGS 300 310 320 330 340 350 370 380 390 g597.pep SGSLPDGEEGLYLQIRYRGQVLΝPSGWIRX I I I I I I I I I I I I I I I I I : I I I I I I l I I I m597 SGΞLPDGEEGLYLQIRYQGQVLΝPSSWIRX 360 370 380 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1881 > a597. seq 1 ATGCTGCTTC ATGTCAGCAA TTCCCTCAAG CAGCTTCAGG AAGAGCGTAT 51 CCGCCAAGAA CGTATCCGCC AAGAGCGTAT CCGTCAGGCG CGCGGCAACC 101 TTGCTTCCGT CAACCGCAAA CAGCGCGAGG CTTGGGACAA GTTCCAAAAA 151 CTCAATACCG AGCTGAACCG TTTGAAAACG GAAGTCGCCG CTACGAAAGC 201 GCAGATTTCC CGTTTCGTAT CGGGGAACTA TAAAAACAGC CAGCCGAATG 251 CGGTTGCCCT GTTCCTGAAA AACGCCGAAC CGGGTCAGAA AAACCGCTTT 301 TTGCGTTATA CGCGTTATGT AAACGCCTCC AATCGGGAAG TTGTCAAGGA 351 TTTGGAAAAA CAGCAGAAGG CTTTGGCGGT ACAAGAGCAG AAAATCAACA 401 ATGAGCTTGC CCGTTTGAAG AAAATTCAGG CAAACGTGCA ATCCCTGCTG 451 AAAAAACAGG GTGTAACCGA TGCGGCGGAA CAGACGGAAA GCCGCAGACA 501 GAATGCCAAA ATCGCCAAAG ATGCCCGAAA ACTGCTGGAA CAGAAAGGGA 551 ACGAGCAGCA GCTGAACAAG CTCTTGAGCA ATTTGGAGAA GAAAAAGGCC 601 GAACACCGCA TTCAGGATGC GGAAGCAAAA AGAAAATTGG CTGAAGCCAG 651 ACTGGCGGCA GCCGAAAAAG CCAGAAAAGA AGCGGCGCAG CAGAAGGCTG 701 AAGCACGACG TGCGGAAATG TCCAACCTGA CCGCCGAAGA CAGGAACATC 751 CAAGCGCCTT CGGTTATGGG TATCGGCAGT GCCGACGGTT TCAGCCGCAT 801 GCAAGGACGT TTGAAAAAAC CGGTTGACGG TGTGCCGACC GGACTTTTCG 851 GGCAGAACCG GAGCGGCGGC GATGTTTGGA AAGGCGTGTT CTATTCCACT 901 GCACCGGCAA CGGTTGAAAG CATTGCGCCG GGAACGGTAA GCTATGCGGA 951 CGAGTTGGAC GGCTACGGCA AAGTGGTCGT GGTCGATCAC GGCGAGAACT 1001 ACATCAGCAT CTATGCCGGT TTGAGCGAAA TTTCCGTCGG CAAGGGTTAT 1051 ATGGTCGCGG CAGGAAGCAA AATCGGCTCG AGCGGGTCGC TGCCGGACGG 1101 GGAAGAGGGG CTTTACCTGC AAATACGTTA TCAAGGTCAG GTATTGAACC 1151 CTTCGAGCTG GATACGTTGA This corresponds to the amino acid sequence <SEQ ID 1882; ORF 597. a>: a597.pep 1 MLLHVSNSLK QLQEERIRQE RIRQERIRQA RGNLAΞVNRK QREAWDKFQK 51 LNTELNRLKT EVAATKAQIS RFVSGNYKNS QPNAVALFLK NAEPGQKNRF 101 LRYTRYVNAS NREVVKDLEK QQKALAVQEQ KINNELARLK KIQANVQSLL 151 KKQGVTDAAE QTESRRQNAK IAKDARKLLE QKGNEQQLNK LLSNLEKKKA 201 EHRIQDAEAK RKLAEARLAA AEKARKEAAQ QKAEARRAEM ΞNLTAEDRNI 251 QAPSVMGIGS ADGFSRMQGR LKKPVDGVPT GLFGQNRSGG DVWKGVFYΞT 301 APATVESIAP GTVΞYADELD GYGKVVVVDH GENYISIYAG LSEISVGKGY 351 MVAAGSKIGS SGSLPDGEEG LYLQIRYQGQ VLNPSSWIR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 597 shows 98.5% identity over a 389 aa overlap with a predicted ORF (ORF 597) from N meningitidis 597/a597 98.5% identity in 389 aa overlap 10 20 30 40 50 60 a597.pep MLLHVSΝSLKQLQEERIRQERIRQERIRQARGΝLASVΝRKQREAWDKFQKLΝTELΝRLKT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m597 MLLHVSΝSLKQLQEERIRQERIRQ ARGΝLASVΝRKQREAWDKFQKLΝTELΝRLKT 10 20 30 40 50 70 80 90 100 110 120 a597. pep EVAATKAQISRFVSGΝYKΝSQPΝAVALFLKΝAEPGQKΝRFLRYTRYVΝASΝREVVKDLEK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m597 EVAATKAQISRFVSGΝYKΝSQPΝAVALFLKΝAEPGQKΝRFLRYTRYVΝASΝREVVKDLEK 60 70 80 90 100 110 130 140 150 160 170 180 a597.pep QQKALAVQEQKIΝΝELARLKKIQAΝVQSLLKKQGVTDAAEQTESRRQΝAKIAKDARKLLE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m597 QQKALAVQEQKIΝΝELARLKKIQAΝVQSLLKKQGVTDAAEQTESRRQΝAKIAKDARKLLE 120 130 140 150 160 170 190 200 210 220 230 240 a597.pep QKGΝEQQLΝKLLSΝLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRAEM I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m597 QKGΝEQQLΝKLLSΝLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRAEM 180 190 200 210 220 230 250 260 270 280 290 300 a597.pep SΝLTAEDRΝIQAPΞVMGIGΞADGFSRMQGRLKKPVDGVPTGLFGQΝRSGGDVWKGVFYST I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I m597 SΝLTAEDRΝIQAPΞVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQΝRΞGGDIWKGVFYST 240 250 260 270 280 290 310 320 330 340 350 360 a597.pep APATVESIAPGTVSYADELDGYGKVVVVDHGEΝYIΞIYAGLSEISVGKGYMVAAGSKIGS I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m597 APATVESIAPGTVSYADELDGYGKVVVVDHGEΝYISIYAGLΞEISVGKGYMVAAGSKIGS 300 310 320 330 340 350 370 380 390 a597.pep SGSLPDGEEGLYLQIRYQGQVLΝPSSWIRX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m597 SGΞLPDGEEGLYLQIRYQGQVLΝPSSWIRX 360 370 380 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1883>: gδOl.seq 1 ATGTTCCCAA CCGGCAATTT GGTCGACGAA ATTGATGTGC CGAATATAGG 51 TCGTCTGAAA GCCACGCTCA TCAACGCGGG CATTCCGACC GTTTTCCTGA 101 ACGCCGCCGA CTTGGGCTAC ACGGGCAAAG AGTTGCAGGA CGACATCAAC 151 AACGATGCCG CCGCGCTGGA AAAATTTGAA ACCATCCGCG CATATGGCGC 201 GCTGAAAATG GGTTTGATCA GCGACGTATC CGAAGCCGCC GCCCGCGCGC 251 GCACGCCGAA ACCCGCCTTC GTCGCGCCCG CCGCCGATTA CACCGCCTCC 301 AGCGGCAAAA CCGTAAACGC CGCCGACATC GATTTGCCGG TACGCGCCCT 351 GAGCATGGGC AAACTGCACC ACGCTATGAT GGGCATCGCC TCGGTCGCCA 401 TCGCCGCCGC CGTGCTCGGT ACGCTGGTCA ACCTTGCCGC AGGCGGCGGA 451 ACGCGTAAAG AAGTGCGCTT CGGGCATCCG TCAGGTACGC TGCGTGTCGG 501 TGCTGCCGCC GAATGTCAGG ACGGACAATG GACGGCCGCc aaagcggtca 551 tgaGCCGCAG CGCACgcgtg attatggaaa gttgGGTGCg cgttcccgat 601 gattGTTTTT GA This corresponds to the amino acid sequence <SEQ ID 1884; ORF 601. ng>: g601.pep 1 MFPTGΝLVDE IDVPΝIGRLK ATLIΝAGIPT VFLΝAADLGY TGKELQDDIΝ 51 ΝDAAALEKFE TIRAYGALKM GLISDVSEAA ARARTPKPAF VAPAADYTAS 101 SGKTVΝAADI DLPVRALSMG KLHHAMMGIA SVAIAAAVLG TLVΝLAAGGG 151 TRKEVRFGHP SGTLRVGAAA ECQDGQWTAA KAVMSRSARV IMESWVRVPD 201 DCF* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1885>: m601. seq 1 ATGTTCCCAA CCGGCAATTT GGTCGATGAA ATTGATGTGC CGAATATAGG 51 CCGTTTGAAA GCCACGCTCA TCAACGCGGG CATTCCGACC GTTTTCTTGA 101 ATGCCGCCGA CTTGGGCTAC ACAGGCAAAG AGTTGCAAGA CGACATCAAC 151 AACGATGCCG CGGCTTTGGA AAAATTCGAG AAAATCCGCG CTTACGGTGC 201 GCTGAAAATG GGTCTGATCA GCGACGTATC CGAAGCTGCC GCTCGCGCGC 251 ACACGCCGAA AGTCGCCTTC GTCGCGCCCG CCGCCGATTA CACCGCCTCC 301 AGTGGCAAAA CCGTGAACGC CGCCGACATC GATTTGCTGG TACGCGCCCT 351 GAGCATGGGC AAACTGCACC ACGCGATGAT GGGTACCGCC TCTGTTGCCA 401 TTGCGACCGC CGCCGCCGTA CCCGGTACGC TGGTCAACCT TGCCGCAGGC 451 GGCGGAACGC GTAAAGAAGT GCGCTTCGGG CATCCTTCCG GCACATTGCG 501 CGTCGGTGCA GCCGCCGAAT GTCAGGACGG ACAATGGACG GCCACCAAAG 551 CGGTCATGAG CCGTAGCGCA CGCGTGATGA TGGAAGGTTG GGTCAGGGTG 601 CCTGAGGATT GTTTTTAA This corresponds to the amino acid sequence <SEQ ID 1886; ORF 601>: m601.pep 1 MFPTGΝLVDE IDVPΝIGRLK ATLIΝAGIPT VFLΝAADLGY TGKELQDDIΝ 51 ΝDAAALEKFE KIRAYGALKM GLISDVSEAA ARAHTPKVAF VAPAADYTAS 101 SGKTVΝAADI DLLVRALSMG KLHHAMMGTA SVAIATAAAV PGTLVΝLAAG 151 GGTRKEVRFG HPSGTLRVGA AAECQDGQWT ATKAVMSRSA RVMMEGWVRV 201 PEDCF* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 601 shows 94.1% identity over a 205 aa overlap with a predicted ORF (ORF 601. ng) from N gonorrhoeae: m601/g601 10 20 30 40 50 60 m601.pep MFPTGΝLVDEIDVPΝIGRLKATLIΝAGIPTVFLΝAADLGYTGKELQDDIΝΝDAAALEKFE J I E E I ! 1 1 j [ 1 1 E 1 1 ! 1 1 J 1 1 F j i j r i 1 1 E I J I j E 1 1 j i r r E 1 1 1 1 1 1 j 1 1 1 1 j E I ! r E r g601 MFPTGΝLVDEIDVPΝIGRLKATLIΝAGIPTVFLΝAADLGYTGKELQDDIΝΝDAAALEKFE 10 20 30 40 50 60 70 80 90 100 110 120 m601.pep KIRAYGALKMGLISDVSEAAARAHTPKVAFVAPAADYTASSGKTVΝAADIDLLVRALSMG g601 TIRAYGALKMGLISDVSEAAARARTPKPAFVAPAADYTASSGKTVNAADIDLPVRALSMG 70 80 90 100 110 120 130 140 150 160 170 180 m601. pep KLHHAMMGTASVAIATAAAVPGTLVNLAAGGGTRKEVRFGHPSGTLRVGAAAECQDGQWT I I F I f 111 Mill llll 1111 II 11 II 111 II 111 II 1111111 II I II 1111111 g601 KLHHAMMGIASVAI - -AAAVLGTLVNLAAGGGTRKEVRFGHPSGTLRVGAAAECQDGQWT 130 140 150 160 170 190 200 m601. pep ATKAVMSRSARVMMEGWVRVPEDCFX h l l l l l l l M H l l l l l l l l g601 AAKAVMSRSARVIMESWVRVPDDCFX 180 190 200 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1887>: a601. seq 1 ATGTTCCCAA CCGGCAATTT GGTCGATGAA ATTGATGTGC CGAATATAGG 51 CCGTTTGAAA GCCACGCTCA TCAACGCGGG CATTCCGACC GTTTTCCTGA 101 ATGCCGCCGA CTTGGGCTAC ACGGGCAAAG AGTTGCAAGA CGACATCAAC 151 AACGATGCCG CAGCTTTGGA AAAATTCGAG AAAATCCGCG CTTACGGTGC 201 GCTGAAAATG GGTCTGATCA GCGACGTATC CGAAGCTGCC GCCCGCGCGC 251 ACACGCCGAA AGTCGCCTTC GTCGCGCCCG CCGCCGATTA CACCGCCTCC 301 AGTGGCAAAA CCGTGAATGC CGCCGACATC GATTTGCTGG TACGCGCCCT 351 GAGCATGGGC AAATTGCACC ACGCGATGAT GGGTACCGCC TCTGTTGCCA 401 TTGCGACCGC CGCCGCCGTG CCCGGTACGC TGGTCAACCT TGCCGCAGGC 451 GGCGGAACGC GTAAAGAAGT GCGCTTCGGG CATCCTTCCG GCACATTGCG 501 CGTCGGTGCA GCCGCCGAAT GTCAGGACGG ACAATGGACG GCCACCAAAG 551 CGGTTATGAG CCGCAGCGCA CGCGTGATGA TGGAAGGTTG GGTCAGGGTG 601 CCGGAAGATT GTTTTTAA This corresponds to the amino acid sequence <SEQ ID 1888; ORF 601. a>: a601.pep 1 MFPTGΝLVDE IDVPΝIGRLK ATLIΝAGIPT VFLΝAADLGY TGKELQDDIΝ 51 ΝDAAALEKFE KIRAYGALKM GLISDVSEAA ARAHTPKVAF VAPAADYTAS 101 SGKTVΝAADI DLLVRALSMG KLHHAMMGTA SVAIATAAAV PGTLVΝLAAG 151 GGTRKEVRFG HPSGTLRVGA AAECQDGQWT ATKAVMSRSA RVMMEGWVRV 201 PEDCF* m601/a601 100.0% identity in 205 aa overlap 10 20 30 40 50 60 m601.pep MFPTGΝLVDEIDVPΝIGRLKATLIΝAGIPTVFLΝAADLGYTGKELQDDIΝΝDAAALEKFE II II II II II II II II II II II II I II II I II II II II I I I II II II II II I I I I I M M a601 MFPTGΝLVDEIDVPΝIGRLKATLIΝAGIPTVFLΝAADLGYTGKELQDDIΝΝDAAALEKFE 10 20 30 40 50 60 70 80 90 100 110 120 m601. pep KIRAYGALKMGLISDVSEAAARAHTPKVAFVAPAADYTASSGKTVΝAADIDLLVRALSMG MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a601 KIRAYGALKMGLISDVSEAAARAHTPKVAFVAPAADYTASSGKTVΝAADIDLLVRALSMG 70 80 90 100 110 120 130 140 150 160 170 180 m601. pep KLHHAMMGTASVAIATAAAVPGTLVΝLAAGGGTRKEVRFGHPSGTLRVGAAAECQDGQWT I I I II I I I II I II II II I I II I I II II II I II I I I I I I I I M I M II I I M I I I I M M I a601 KLHHAMMGTASVAIATAAAVPGTLVΝLAAGGGTRKEVRFGHPSGTLRVGAAAECQDGQWT 130 140 150 160 170 180 190 200 m601. pep ATKAVMSRSARVMMEGWVRVPEDCFX II I II II II II II I II II I I I I I I I I a601 ATKAVMSRSARVMMEGWVRVPEDCFX 190 200 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 1889>: g602.seq 1 ATGTTGCTCC ATCAATGCGA CAAAGCGCGA CATATGCGTC CCTTTCTGCT 51 CGGCGGGCAG ATAAACCGTC ATCGTCAGGC GAGCAACCGT GGATTGTGTT 101 CCTTCGGCGG TTTTCAGGGT AATCGGGAAG CGCAGGTCTT TAATGCCGAC 151 CTGATTGATC GGCAGGTTGC GCAAATCTCT GCTGGATTGC ACGTCTGCAA 201 TGGCGTTCAT GCGTTGTTTG TCCTTAATAT TCAGATAATT ATTGAGATGT 251 GTGTATTGTA TGGCAGGcag atgccgtctg aAAAAacgct gtcggCCGCC 301 TGCCTGCAAA TgcgagattA TATCACTTGC TTTtggcgGC TGCATTGA This corresponds to the amino acid sequence <SEQ ID 1890; ORF 602.ng>: g602.pep 1 MLLHQCDKAR HMRPFLLGGQ IΝRHRQASΝR GLCSFGGFQG ΝREAQVFΝAD 51 LIDRQVAQIS AGLHVCΝGVH ALFVLΝIQII IEMCVLYGRQ MPSEKTLΞAA 101 CLQMRDYITC FWRLH* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1891>: 602. seq 1 ATGTTGCTCC ATCAATGCGA CAAAACGCGA CATATGCGTC CCCTTCTGCT 51 CAGCAGGCAG GTAAACCGTC ATGGTCAGAC GGGCAATGGT GGACTGGATG 101 CCTTCTGCAG TTTGCAGGGT AATCGGAAAG CGCAGGTCTT TGATACCGAC 151 CTGATTGATC GGCAGATTGC GCAAATCTCG GCTGGATTGC ACGTCTGCAA 201 TAGTGTTCAT GAGTTGTTTT TCCTTAATAT TCATGTAATT GTTGAGATGT 251 GTGCATGGTA TGGCGTTTCC GCCGGGGAAT ATACCGTCAA TCTGCAAATG 301 CGAGATTATA TCACTCGCTT TTAGCAGCTG CATTGA This corresponds to the amino acid sequence <SEQ ID 1892; ORF 602>: m602.pep 1 MLLHQCDKTR HMRPLLLΞRQ VΝRHGQTGΝG GLDAFCΞLQG ΝRKAQVFDTD 51 LIDRQIAQIS AGLHVCΝSVH ELFFLΝIHVI VEMCAWYGVS AGEYTVΝLQM 101 RDYITRF*QL H* m602/g602 65.2% identity in 115 aa overlap 10 20 30 40 50 60 m602 . pep MLLHQCDKTRHMRPLLLSRQVΝRHGQTGΝGGLDAFCSLQGΝRKAQVFDTDLIDRQIAQIS I I I I I I I I : I I I I I : | I : M i l l M M I I M : : I I I I : I I I I : : I I I I I I : I I I I g602 MLLHQCDKARHMRPFLLGGQIΝRHRQAΞΝRGLCSFGGFQGΝREAQVFΝADLIDRQVAQIS 10 20 30 40 50 60 70 80 90 100 110 m602.pep AGLHVCΝΞVHELFFLΝIHVIVEMCAWYGVSA-GEYTVΝ LQMRDYITRFXQLHX I I I I I I I : I I II I I I : : I : I I I : II ■' : I I : : llllllll I Mil g602 AGLHVCΝGVHALFVLΝIQIIIEMCVLYGRQMPSEKTLSAACLQMRDYITCFWRLHX 70 80 90 100 110 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1893>: a602. seq 1 ATGTTGCTCC ATCAATGCGA CAAAGCGCGA CATATGCGTA CCCTTCTGCT 51 CGGCAGGCAG GTAAACCGTC ATGGTCAGAC GGGCAACTGT GGACTGGATG 101 CCTTCTGCAG TTTGCAGGGT AATCGGAAAG CGCAGGTCTT TGATACCGAC 151 CTGATTGATC GGCAGATTGC GCAAATCTCG GCTGGATTGC ACGTCTGCAA 201 TAGTGTTCAT GAGTTGTTTT TCCTTAATAT TCATGTAATT GTTGAGATGT 251 GTGCATGGTA TGGCGTTTCC ACCGGGGAAT ATACCGTCAA TCTGCAAATG 301 CGAGATTATA TCACTCGCTT TTAGCAGCTG CATTGA This corresponds to the amino acid sequence <SEQ ID 1894; ORF 602. a>: a602.pep 1 MLLHQCDKAR HMRTLLLGRQ VΝRHGQTGΝC GLDAFCSLQG ΝRKAQVFDTD 51 LIDRQIAQIS AGLHVCΝSVH ELFFLΝIHVI VEMCAWYGVS TGEYTVΝLQM 101 RDYITRF*QL H* m602/a602 95.5% identity in ill aa overlap 10 20 30 40 50 60 m602. pep MLLHQCDKTRHMRPLLLSRQVNRHGQTGNGGLDAFCSLQGNRKAQVFDTDLIDRQIAQIS I II I II II : II II MMMMIMMM I II I I II I II I II II II II I II I II II II I a602 MLLHQCDKARHMRTLLLGRQVNRHGQTGNCGLDAFCSLQGNRKAQVFDTDLIDRQIAQIS 10 20 30 40 50 60 70 80 90 100 110 m602. pep AGLHVCNSVHELFFLNIHVIVEMCAWYGVSAGEYTVNLQMRDYITRFXQLHX I II I II II II II I II II I II II I II I II I I : I II I II II II I II I I II I II I a602 AGLHVCNSVHELFFLNIHVIVEMCAWYGVSTGEYTVNLQMRDYITRFXQLHX 70 80 90 100 110 The following partial DNA sequence was identified in N.gonorrhoeae <SEQ ID 1895>: g603.seq 1 ATGGATTCCC GCCTGCGTGG GAATGACGCT AGGAAATACG GCATACGCTT 51 TGCCCAAAGA GGCCGTCTGA AACACACTCC GCCCAACGCC CATCCTTTTT 101 CAGACGGCCC CGCACCAAAA AAACAACCAC AAACTACAAG GAGAAACATC 151 ATGTCCGACC AACTCATTCT TGTCCTGAAC TGCGTCAGTT CATCGCTCAA 201 AGGCGCCGTT ATCGACCGCA AAAGCGGCAG CGTCGTCCTA AGCTGCCTCG 251 GGGAACGCCT GACTACGCCC GAAGCCGTCA TTACCTTCAA CAAAGACGGC 301 AACAAACGCC AAGTTCCCCT GAGCGGCCGC AACTGCCACG CCGGCGCGGT 351 GGGTATGCTG TTGAACGAAC TGGAAAAACA CGGACTGCAC GACCGCATCA 401 AAGCCATCGG CCGCCGCATC GCCCACGGCG GCGAAAAATA TCACGAGTCC 451 GTCCTCATCG ACCAAGACGT CCTTGACGAA CTGAAAGCCT GCATCCCGTT 501 CGCCCCGCTG CACAACCCCG CCAACATCAG CGGCATCCTC GCCGCGCAGG 551 AACACTTTCC CGGCCTGCCC AACGTCGGCG TGATGGACAC CTCGTTCCAC 601 CAAACCATGC CGGAGCGGGC CTACACTTAT GCCGTGCCGC GCGAATTGCG 651 CAAAAAATAC GCCTTCCGCC GCTACGGTTT CCACGGTACC GGTATGCGTT 701 ACGTCGCCCC TGAAGCCGCA CGCATCTTGG GCAAACCTct ggaaGACATC 751 CGCATGATTA TTGCCCACTT AGGCAACGGC GCATCTATTA CCGCCGTCAA 801 AAACGGCAAA TCCGTCGATA CCGGTATGGG TTTCACGCCG ATCGAAGGTT 851 TGGTAATGGG TACACGTTGC GGCGACACCG ATCCGGGCGT ATACAGCTAT 901 CCGACTTTCC ACGCAGGGAT GGATGTTGCC CAAGTTGATG AAATGCTGAA 951 CGAAAAATCA GGTTTCCCCG GTATTTCcgA actTCCCAAC GACTGCCGCA 1001 CCCTCGAAAT CGCCGCCGAC GAAGGCCGCG AAGGCGCGCG CCTCGCCCTC 1051 gaAGTCATGA CCTGCCGCCT CGCCAAATAC ATCGCTTCGA TGGCTGTGGC 1101 CTGCGGCAGT GTTGACGCAC TCGTGTTCAC CGGCGGTATC GGCGAAAACT 1151 CGCGTAATAT CCGTGCCAAA ACCGTTTCCT ATCTTGATTT CTTGGGTCTG 1201 CACATCGACA CCAAAGCCAA TATGGAAAAA CGCTACGGCA ATTCGGGCAT 1251 TATCAGCCCG ACCGATTCTT CTCCGGCTGT TTTGGTCGTC CCGACCAATG 1301 AAGAACTGAT GATTGCCTGC GACACTGCCG AACTTGCCGG CATCTTGTAG This corresponds to the amino acid sequence <SEQ ID 1896; ORF 603.ng>: g603.pep 1 MDSRLRGNDA RKYGIRFAQR GRLKHTPPNA HPFSDGPAPK KQPQTTRRNI 51 MSDQLILVLN CVSSSLKGAV IDRKSGSWL SCLGERLTTP EAVITFNKDG 101 NKRQVPLSGR NCHAGAVGML LNELEKHGLH DRIKAIGRRI AHGGEKYHES 151 VLIDQDVLDE LKACIPFAPL HNPANISGIL AAQEHFPGLP NVGVMDTSFH 201 QTMPERAYTY AVPRELRKKY AFRRYGFHGT GMRYVAPEAA RILGKPLEDI 251 RMIIAHLGNG ASITAVKNGK SVDTGMGFTP IEGLVMGTRC GDTDPGVYSY 301 PTFHAGMDVA QVDEMLNEKS GFPGISELPN DCRTLEIAAD EGREGARLAL 351 EVMTCRLAKY IASMAVACGS VDALVFTGGI GENSRNIRAK TVSYLDFLGL 401 HIDTKANMEK RYGNSGIISP TDSSPAVLW PTNEELMIAC DTAELAGIL* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 1897>: m603. seq 1 CTGTCCTCGC GTAGGCGGGG ACGGAATAAC GATAGAAAAT GCGGCATACG 51 CTTTGCCCAA AGAGGCCGTC TGAAACACCT TGCGCCTGAT GTCTGC . CTT 101 TTTCAGACGA CCCCACACTA AAAAAACAAC CACAAACTAC AAGGAGAAAC 151 ATCATGTCCG ACCAACTCAT CCTCGTTCTG AACTGCGGCA GTTCATCGCT 201 CAAAGGCGCC GTTATCGACC GAmAAAGCGG CAGCGTCGTC CTAAGCTGCC 251 TCGGCGAACG cCtGACCACG CCCGAAGCCG TCATTACGTT CAACAAAGAC 301 GGCAACAAAC GCCAAGTTCC CCTGAGCGGC CGAAATTGCC ACGCCGGCGC 351 GGTGGGTATG CTTTTGAACG AACTGGAAAA ACACGGTCTG CACGACCGCA 401 TCAAAGCCAT CGGCCACCGC ATCGCCCACG GCGGCGAAAA ATACAGCGAG 451 TCTGTTTTGA TCGACCAGGC CGTAATGGAC GAACTCAATG CCTGCATTCC 501 GCTTGCGCCG CTGCACAACC CCGCCAACAT CAGCGGCATC CTTGCCGCAC 551 AGGAACATTT CCCCGGTCTG CCCAATGTCG GCGTGATGGA TACTTCGTTC 601 CACCAAACCA TGCCGGAGCG TGCCTACACT TATGCCGTGC CGCGCGAGTT 651 GCGTAAAAAA TACGCTTTCC GCCGCTACGG TTTCCACGGC ACCAGTATGC 701 GTTACGTTGC CCCTGAAGCC GCACGCATCT TGGGCAAACC TCTGGAAGAC 751 ATCCGCATGA TTATTGCCCA CTTAGGCAAC GGCGCATCCA TTACCGCCAT 801 CAAAAACGGC AAATCCGTCG ATACCAGTAT GGGTTTCACG CCGATCGAAG 851 GTTTGGTAAT GGGTACACGT TGCGGCGACA TCGATCCGGG CGTATACAGC 901 TATCTGACTT CCCACGCCGG GATGGATGTT GCCCAAGTGG ATGAAATGCT 951 GAACAAAAAA TCAGGTTTGC TCGGTATTTC CGAACTTTCC AACGACTGCC 1001 GCACCCTCGA AATCGCCGCC GACGAAGGCC ACGAAGGCGC GCGCCTCGCC 1051 CTCGAAGTCA TGACCTACCG CCTCGCCAAA TACATCGCTT CGATGGCTGT 1101 GGGCTGCGGC GGCGTTGACG CACTCGTGTT CACCGGCGGT ATCGGCGAAA 1151 ACTCGCGTAA TATCCGTGCC AAAACCGTTT CCTATCTTGA TTTCTTGGGT 1201 CTGCACATCG ACACCAAAGC CAATATGGAA AAACGCTACG GCAATTCGGG 1251 CATTATCAGC CCGACCGATT CTTCTCCGGC TGTTTTGGTT GTCCCGACCA 1301 ATGAAGAACT GATGATTGCC TGCGACACTG CCGAACTTGC CGGCATCTTG 1351 TAG This corresponds to the amino acid sequence <SEQ ID 1898; ORF 603>: m603.pep 1 LSSRRRGRNN DRKCGIRFAQ RGRLKHLAPD VCXFSDDPTL KKQPQTTRRN 51 IMSDQLILVL NCGSSSLKGA VIDRXSGSW LSCLGERLTT PEAVITFNKD 101 GNKRQVPLSG RNCHAGAVGM LLNELEKHGL HDRIKAIGHR IAHGGEKYSE 151 SVLIDQAVMD ELNACIPLAP LHNPANISGI LAAQEHFPGL PNVGVMDTSF 201 HQTMPERAYT YAVPRELRKK YAFRRYGFHG TSMRYVAPEA ARILGKPLED 251 IRMIIAHLGN GASITAIKNG KSVDTSMGFT PIEGLVMGTR CGDIDPGVYS 301 YLTSHAGMDV AQVDEMLNKK SGLLGISELS NDCRTLEIAA DEGHEGARLA 351 LEVMTYRLAK YIASMAVGCG GVDALVFTGG IGENSRNIRA KTVSYLDFLG 401 LHIDTKANME KRYGNSGIIS PTDSSPAVLV VPTNEELMIA CDTAELAGIL 451 * Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 603 shows 91.6% identity over a 450 aa overlap with a predicted ORF (ORF 603.ng) from N gonorrhoeae: m603/g603 10 20 30 40 50 60 m603. pep LSSRRRGRΝΝDRKCGIRFAQRGRLKHLAPDVCXFSDDPTLKKQPQTTRRΝIMSDQLILVL : = l l I I M M I I I M I I I M M h = M l h I I I I I I I I I I 1 1 I I I I I I I I g603 MDSRLRG-ΝDARKYGIRFAQRGRLKHTPPΝAHPFSDGPAPKKQPQTTRRΝIMSDQLILVL 10 20 30 40 50 70 80 90 100 110 120 m603. pep ΝCGSSSLKGAVIDRXSGSWLSCLGERLTTPEAVITFΝKDGΝKRQVPLSGRΝCHAGAVGM II I ! 1111 j I II ! II I II II II II II II II II II I II I II I I II II I II II I II I II I g603 ΝCVSSSLKGAVIDRKSGSWLSCLGERLTTPEAVITFΝKDGΝKRQVPLSGRΝCHAGAVGM 60 70 80 90 100 110 130 140 150 160 170 180 m603 . pep LLΝELEKHGLHDRIKAIGHRIAHGGEKYSESVLIDQAVMDELΝACIPLAPLHΝPAΝISGI 11 M 1111 M 11 M 11 II : 111111111 h II h 111 h II II II II II II g603 LLΝELEKHGLHDRIKAIGRRIAHGGEKYHESVLIDQDVLDELKACIPFAPLHΝPAΝISGI 120 130 140 150 160 170 190 200 210 220 230 240 m603. pep LAAQEHFPGLPΝVGVMDTSFHQTMPERAYTYAVPRELRKKYAFRRYGFHGTSMRYVAPEA g603 LAAQEHFPGLPNVGVMDTSFHQTMPERAYTYAVPRELRKKYAFRRYGFHGTGMRYVAPEA 180 190 200 210 220 230 250 260 270 280 290 300 m603. pep ARI GKPLEDIRMI IAHLGNGASITAIKNGKSVDTSMGFTPIEGLVMGTRCGDIDPGVYS M M M g603 ARILGKPLEDIRMIIAHLGNGASITAVKNGKSVDTGMGFTPIEGLVMGTRCGDTDPGVYS 240 250 260 270 280 290 310 320 330 340 350 360 m603.pep YLTSHAGMDVAQVDEMLNKKSGLLGISELSNDCRTLEIAADEGHEGARLALEVMTYRLAK I I M M M M M M I h l l l : M i l l 1 1 1 I I I I I I I I I h I I I I I I I I I I I M M g603 YPTFHAGMDVAQVDEMLNEKSGFPGISELPNDCRTLEIAADEGREGARLALEVMTCRLAK 300 310 320 330 340 350 370 380 390 400 410 420 m603.pep YIASMAVGCGGVDALVFTGGIGENSRNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIIS I ! 1 1 1 1 1 : 1 i : 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 ! 1 1 1 1 1 1 1 1 1 1 1 1 1 1 ! 1 1 1 1 1 1 1 1 M 1 1 g603 YIASMAVACGSVDALVFTGGIGENSRNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIIS 360 370 380 390 400 410 430 440 450 IΪ1603 . pep PTDSSPAVLWPTNEELMIACDTAELAGILX 1 1 1 1 M I ! 1 1 M 1 1 I I 1 1 i 1 1 1 I I I i 1 1 1 1 1 g603 PTDSSPAVLWPTNEELMIACDTAELAGILX 420 430 440 450 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1899>: a603. seq 1 CTGTCCTCGC GTAGGCGGGG ACGGAATAAC GATAGAAAAT GCGGCATACG 51 CTTTGCCCAA AGAGGCCGTC TGAAACACAC TCCGCCCAAC GCCCATCCTT 101 TTTCAGACGA CCCCACACC . AAAAAACAAC CACAAACTAC AAGGAGAAAC 151 ATCATGTCCG ACCAACTCAT TCTTGTTCTG AACTGCGGCA GTTCATCGCT 201 CAAAGGTGCC GTTATCGACC GCAAAAGCGG CAGCGTCGTC CTAAGCTGCC 251 TCGGCGAACG CCTGACCACG CCCGAAGCCG TCATTACGTT CAGCAAAGAC 301 GGCAACAAAC GCCAAGTTCC CCTGAGCGGC CGGAACTGCC ACGCCGGCGC 351 GGTGGGTATG CTGTTGAACG AACTGGAAAA ACACGAACTG CACGACCGCA 401 TTCAAGCCGT CGGCCACCGC ATCGCCCACG GCGGCGAAAA ATACAGCGAG 451 TCTGTTTTGA TCGACCAGGC CGTAATGGAC GAACTCAATG CCTGCATTCC 501 GCTTGCGCCG CTGCACAACC CCGCCAACAT CAGCGGCATC CTCGCCGCAC 551 AGGAACATTT CCCCGGTCTG CCCAATGTCG GCGTGATGGA TACTTCGTTC 601 CACCAAACCA TGCCGGAGCG TGCCTACACT TATGCCGTGC CGCGCGAGTT 651 GCGTAAAAAA TACGCTTTCC GCCGCTACGG TTTCCACGGC ACCAGTATGC 701 GTTACGTTGC CCCTGAAGCC GCATGCATCT TGGGCAAACC TCTGGAAGAC 751 ATCCGCATGA TTATTGCCCA CTTAGGCAAC GGCGCATCCA TTACCGCCAT 801 CAAAAACGGC AAATCCGTCG ATACCAGTAT GGGTTTCACG CCGATCGAAG 851 GTTTGGTAAT GGGTACGCGC TGCGGCGATA TCGACCCGGG CGTATACAGC 901 TATCTGACTT CACACGCCGG TTTGGATGTT GCACAAGTTG ATGAAATGCT 951 GAATAAAAAA TCAGGCTTGC TCGGTATTTC CGAACTCTCC AACGACTGCC 1001 GCACCCTCGA AATCGCCGCC GACGAAGGCC ACGAAGGCGC GCGCCTCGCC 1051 CTCGAAGTTA TGACCTACCG CCTCGCCAAA TACATCGCTT CGATGGCTGT 1101 GGGCTGCGGC GGCGTTGACG CACTCGTGTT CACCGGCGGT ATCGGCGAAA 1151 ACTCGCGTAA TATCCGTGCC AAAACCGTTT CCTATCTTGA TTTCTTGGGT 1201 CTGCACATCG ACACCAAAGC CAATATGGAA AAACGCTACG GCAATTCGGG 1251 TATTATCAGC CCGACCGATT CTTCTCCGGC TGTTTTGGTT GTCCCGACCA 1301 ATGAAGAACT GATGATTGCC TGCGACACTG CCGAACTTGT CGGCATCTTG 1351 TAG This corresponds to the amino acid sequence <SEQ ID 1900; ORF 603. a>: a603.pep 1 LSSRRRGRΝΝ DRKCGIRFAQ RGRLKHTPPΝ AHPFSDDPTX KKQPQTTRRΝ 51 IMSDQLILVL ΝCGSSSLKGA VIDRKSGSVV LSCLGERLTT PEAVITFSKD 101 GΝKRQVPLSG RΝCHAGAVGM LLΝELEKHEL HDRIQAVGHR IAHGGEKYSE 151 SVLIDQAVMD ELΝACIPLAP LHΝPAΝISGI LAAQEHFPGL PΝVGVMDTSF 201 HQTMPERAYT YAVPRELRKK YAFRRYGFHG TSMRYVAPEA ACILGKPLED 251 IRMIIAHLGN GASITAIKNG KSVDTSMGFT PIEGLVMGTR CGDIDPGVYS 301 YLTSHAGLDV AQVDEMLNKK SGLLGISELS NDCRTLEIAA DEGHEGARLA 351 LEVMTYRLAK YIASMAVGCG GVDALVFTGG IGENSRNIRA KTVSYLDFLG 401 LHIDTKANME KRYGNSGIIS PTDSSPAVLV VPTNEELMIA CDTAELVGIL 451 * m603/a603 96.7% identity in 450 aa overlap 10 20 30 40 50 60 m603. pep LSSRRRGRNNDRKCGIRFAQRGRLKHLAPDVCXFSDDPTLKKQPQTTRRNIMSDQLILVL II I I I II II II I II II II I II II I II I : : II II II I II I I I I I I I I II I a603 LSSRRRGRNNDRKCGIRFAQRGRLKHTPPNAHPFSDDPTXKKQPQTTRRNIMSDQLILVL 10 20 30 40 50 60 70 80 90 100 110 120 m603. pep NCGSSSLKGAVIDRXSGSWLSCLGERLTTPEAVITFNKDGNKRQVPLSGRNCHAGAVGM II I I I II II II I II II II II II I II II I II II II II : I II II II II II I II II II II I I a603 NCGSSSLKGAVIDRKSGSWLSCLGERLTTPEAVITFSKDGNKRQVPLSGRNCHAGAVGM 70 80 90 100 110 120 130 140 150 160 170 180 m603. pep LLNELEKHGLHDRIKAIGHRIAHGGEKYSESVLIDQAVMDELNACIPLAPLHNPANISGI llllllll I II I I : I : II II II I II II I II I I I II II II II II I I II I I II I II I I I I I a603 LLNELEKHELHDRIQAVGHRIAHGGEKYSESVLIDQAVMDELNACIPLAPLHNPANISGI 130 140 150 160 170 180 190 200 210 220 230 240 m603. pep LAAQEHFPGLPNVGVMDTSFHQTMPERAYTYAVPRELRKKYAFRRYGFHGTSMRYVAPEA I I I I I II II I I II II II II II II I II II I II II II II II I II I II II I II II I II II II I a603 LAAQEHFPGLPNVGVMDTSFHQTMPERAYTYAVPRELRKKYAFRRYGFHGTSMRYVAPEA 190 200 210 220 230 240 250 260 270 280 290 300 m603.pep ARILGKPLEDIRMIIAHLGNGASITAIKNGKSVDTSMGFTPIEGLVMGTRCGDIDPGVYS I II I II II II I II II II II I II I II II I II II I II II II II II II I II II I I II II II I a603 ACILGKPLEDIRMIIAHLGNGASITAIKNGKSVDTSMGFTPIEGLVMGTRCGDIDPGVYS 250 260 270 280 290 300 310 320 330 340 350 360 m603. pep YLTSHAGMDVAQVDEMLNKKSGLLGISELSNDCRTLEIAADEGHEGARLALEVMTYRLAK MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a603 YLTSHAGLDVAQVDEMLNKKSGLLGISELSNDCRTLEIAADEGHEGARLALEVMTYRLAK 310 320 330 340 350 360 370 380 390 400 410 420 m603.pep YIASMAVGCGGVDALVFTGGIGENSRNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIIS II II II II II II I II II II I II II II II II II I II I II I II I II II I II II I I II II II I a603 YIASMAVGCGGVDALVFTGGIGENSRNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIIS 370 380 390 400 410 420 430 440 450 m603. pep PTDSSPAVLWPTNEELMIACDTAELAGILX II I II I II II II II II II II I II II I : II II a603 PTDSSPAVLWPTNEELMIACDTAELVGILX 430 440 450 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1901>: g604. seq 1 ATGCCCGAAG CGCACTTCTT TACGCGTTCC GCCGCCTGCG GCAAGGTTGA 51 CCAGCGTACC GAGCACGGCG GCGGCGATGG CGACCGAGGC GATGCCCATC 101 ATAGCGTGGT GCAGTTTGCC CATGCTCAGG GCGCGTACCG GCAAATCGAT 151 GTCGGCGGCG TTTACGGTTT TGCCGCTGGA GGCGGTGTAA TCGGCGGCGG 201 GCGCGACGAA GGCGGGTTTC GGCGTGCGCG CGCGGGCGGC GGCTTCGGAT 251 ACGTCGCTGA TCAAACCCAT TTTCAGCGCG CCATATGCGC GGATGGTTTC 301 AAATTTTTCC AGCGCGGCGG CATCGTTGTT GATGTCGTCC TGCAACTCTT 351 TGCCCGTGTA GCCCAAGTCG GCGGCGTTCA GGAAAACGGT CGGAATGCCC 401 GCGTTGATGA GCGTGGCTTT CAGACGACCT ATATTCGGCA CATCAATTTC 451 GTCGACCAAA TTGCCGGTTG GGAACATACT GCCTTcgcCG TCGGCTGGAT 501 CTAA This corresponds to the amino acid sequence <SEQ ID 1902; ORF 604.ng>: g604.pep 1 MPEAHFFTRS AACGKVDQRT EHGGGDGDRG DAHHSWQFA HAQGAYRQID 51 VGGVYGFAAG GGVIGGGRDE GGFRRARAGG GFGYVADQTH FQRAICADGF 101 KFFQRGGIW DWLQLFARV AQVGGVQENG RNARVDERGF QTTYIRHINF 151 VDQIAGWEHT AFAVGWI* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1903>: m604.seq 1 ATGCCCGAAG CGCACTTCTT TACGCGTTCC GCCGCCTGCG GCAAGGTTGA 51 CCAGCGTACC GGGTACGGCG GCGGCGGTCG CAATGGCAAC AGAGGCGGTA 101 CCCATCATCG CGTGGTGCAG TTTGCCCATG CTCAGGGCGC GTACCAGCAA 151 ATCGATGTCG GCGGCGTTCA CGGTTTTGCC ACTGGAGGCG GTGTAATCGG 201 CGGCGGGCGC GACGAAGGCG ACTTTCGGCG TGTGCGCGCG AGCGGCAGCT 251 TCGGATACGT CGCTGATCAG ACCCATTTTC AGCGCACCGT AAGCGCGGAT 301 TTTCTCGAAT TTTTCCAAAG CCGCGGCATC GTTGTTGATG TCGTCTTGCA 351 ACTCTTTGCC TGTGTAGCCC AAGTCGGCGG CATTCAAGAA AACGGTCGGA 401 ATGCCCGCGT TGATGAGCGT GGCTTTCAAA CGGCCTATAT TCGGCACATC 451 AATTTCATCG ACCAAATTGC CGGTTGGGAA CATACTGCCT TCGCCGTCGG 501 CTGGATC This corresponds to the amino acid sequence <SEQ ID 1904; ORF 604>: m604.pep 1 MPEAHFFTRS AACGKVDQRT GYGGGGRΝGΝ RGGTHHRWQ FAHAQGAYQQ 51 IDVGGVHGFA TGGGVIGGGR DEGDFRRVRA SGSFGYVADQ THFQRTVSAD 101 FLEFFQSRGI WDWLQLFA CVAQVGGIQE ΝGRΝARVDER GFQTAYIRHI 151 ΝFIDQIAGWE HTAFAVGWI Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 604 shows 83.4% identity over a 169 aa overlap with a predicted ORF (ORF 604.ng) from N gonorrhoeae: m604/g604 10 20 30 40 50 60 m604.pep MPEAHFFTRSAACGKVDQRTGYGGGGRΝGΝRGGTHHRWQFAHAQGAYQQIDVGGVHGFA M M I M M I M M M M M M M A A \ A \ I I I I I I I I I I I A I I I I I 1 = I I I g604 MPEAHFFTRSAACGKVDQRTEHGGG- -DGDRGDAHHSWQFAHAQGAYRQIDVGGVYGFA 10 20 30 40 50 70 80 90 100 110 120 m604.pep TGGGVIGGGRDEGDFRRVRASGSFGYVADQTHFQRTVSADFLEFFQSRGIWDWLQLFA M I M M M M M I I I = I I : I I 1 1 1 1 1 1 1 1 1 1 : : I I - I I I 1 1 1 1 1 1 1 1 1 1 1 1 g604 AGGGVIGGGRDEGGFRRARAGGGFGYVADQTHFQRAICADGFKFFQRGGIWDWLQLFA 60 70 80 90 100 110 130 140 150 160 169 m604.pep CVAQVGGIQEΝGRΝARVDERGFQTAYIRHIΝFIDQIAGWEHTAFAVGWI g604 RVAQVGGVQEΝGRΝARVDERGFQTTYIRHIΝFVDQIAGWEHTAFAVGWIX 120 130 140 150 160 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1905>: a604. seq 1 ATGCCCGAAG CGCACTTCTT TACGCGTTCC GCCGCCTGCG GCAAGGTTGA 51 CCAGCGTACC GGGCACGGCG GCGGCGGTCG CAATGGCAAC AGAGGCGGTA 101 CCCATCATCG CGTGGTGCAA TTTGCCCATG CTCAGGGCGC GTACCAGCAA 151 ATCGATGTCG GCGGCATTCA CGGTTTTGCC ACTGGAGGCG GTGTAATCGG 201 CGGCGGGCGC GACGAAGGCG ACTTTCGGCG TGTGCGCGCG GGCGGCAGCT 251 TCGGATACGT CGCTGATCAG ACCCATTTTC AGCGCACCGT AAGCGCGGAT 301 TTTCTCGAAT TTTTCCAAAG CTGCGGCATC GTTGTTGATG TCGTCTTGCA 351 ACTCTTTGCC CGTGTAGCCC AAGTCGGCGG CATTCAGGAA AACGGTCGGA 401 ATGCCCGCGT TGATGAGCGT GGCTTTCAAA CGGCCTATAT TCGGCACATC 451 AATTTCATCG ACCAAATTGC CGGTTGGGAA CATACTGCCT TCGCCGTCGG 501 CTGGATCAAG AAATTCGATT TGTACTTCGG CTGCCGGGAA CGTTACGCCG 551 TCGAGCTCAA AATCGCCTGT TTCCAAAACT GCGCCGTTTT GCATCGGTAC 601 ATGGGCAATA ATGGTTTTGC CGATGTTTTT CTGCCAGATT TTGACTGTGC 651 AGATGCCGTC TGA This corresponds to the amino acid sequence <SEQ ID 1906; ORF 604.a>: a604.pep 1 MPEAHFFTRS AACGKVDQRT GHGGGGRNGN RGGTHHR Q FAHAQGAYQQ 51 IDVGGIHGFA TGGGVIGGGR DEGDFRRVRA GGSFGYVADQ THFQRTVSAD 101 FLEFFQSCGI WDWLQLFA RVAQVGGIQE NGRNARVDER GFQTAYIRHI 151 NFIDQIAGWE HTAFAVGWIK KFDLYFGCRE RYAVELKIAC FQNCAVLHRY 201 MGNNGFADVF LPDFDCADAV * m604/a604 97.0% identity in 169 aa overlap 10 20 30 40 50 60 m604 . pep MPEAHFFTRSAACGKVDQRTGYGGGGRNGNRGGTHHRWQFAHAQGAYQQIDVGGVHGFA M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M a604 MPEAHFFTRSAACGKVDQRTGHGGGGRNGNRGGTHHRWQFAHAQGAYQQIDVGGIHGFA 10 20 30 40 50 60 70 80 90 100 110 120 m604. pep TGGGVIGGGRDEGDFRRVRASGSFGYVADQTHFQRTVSADFLEFFQSRGIWDWLQLFA II I I II II II II II I I II II : II I II II II II II II I II I I II II II II I II I I II I I I a604 TGGGVIGGGRDEGDFRRVRAGGSFGYVADQTHFQRTVSADFLEFFQSCGIWDWLQLFA 70 80 90 100 110 120 130 140 150 160 169 m604.pep CVAQVGGIQENGRNARVDERGFQTAYIRHINFIDQIAGWEHTAFAVGWI I I I II I II II II II II II II I I II II II II I II II I II I II II II II I a604 RVAQVGGIQENGRNARVDERGFQTAYIRHINFIDQIAGWEHTAFAVGWIKKFDLYFGCRE 130 140 150 160 170 180 a604 RYAVELKIACFQNCAVLHRYMGNNGFADVFLPDFDCADAVX 190 200 210 220 The following partial DNA sequence was identified in N.gonorrhoeae <SEQ ID 1907>: g605. seq 1 ATGATGACCG AAATGCAACA ACGCGCCCAA CTGCACCGCC AAATTTGGAA 51 AATCGCCGAC GAAGTACGCG GCGCGGTGGA TGGCTGGGAC TTTAAACAAT 101 ACGTTCTCGG CACACTTTTC TACCGCTTTA TCAGCGAAAA CTTCACCGAC 151 TATATGCAGG CCGGCGACAG CAGCATTGAT TACGCCGCta tGCCGGACAG 201 CATCATCACG CCCGAAATCA AAGACGATgc cgtcaaagtc aaAGGCTATT 251 TCATCtacCc cgGCCAGCTT TTTTgcaata ttgccgccga agcCCATCAA 301 AACGAAGAGC TCAACACCAA GCTGAAAGAa atCTTTACCG CGATTGAAAG 351 CTCCGCCTCC GGCTAcccgT CCGAACAAGG CATCAAAGGC TTGTTTGACG 401 ACTTCgACAC CACCAGCAGC CGGCTCGGCA GCACCGTTGC CGACAAAAAC 451 AAACGCCTTG CCGCCGTCCT TAAAGGCGTG GCGGAACTCG ATTTCGGCAA 501 TTTTGAAGAC CACCGCATCG ACCTTTTCGG TGATGCCTAC GAATACCTGA 551 TTTCCAACTA CGCcgcCAAC GCAGGCAAAT CCGGCGGCGA ATTTTTCACC 601 CCGCAAAGCG TCTCCAAGCT GATTGCGCGG CTGGCGGTGC ACGGGCAGGA 651 GAAAGTCAAC AAAATCTACG ACCCCGCCTG CGGCTCGGGC AGCCTGCTCT 701 TGCAGGCGAA AAAACAGTTT GACGAACACA TCATCGAAGA AGGCTTCTTC 751 GGGCAGGAAA TCAACCACAC CACCTACAAC CTCGCCCGCA TGAATATGTT 801 TCTGCACAAC GTCAATTACA ACAAATTCCA CATCGAATTG GGCGACACGC 851 TGACCAACCC CAAACTCAAA GACAGCAAAC CCTTTGATGC CGTCGTCTCC 901 AATCCGCCCT ATTCCATCGA CTGGATAGGC AGCGACGACC CCACCTtgaT 951 CAACGACGAC CGCTTTGCCC CCGCAGGCGT ACTCGCACCG AAATCCAAAG 1001 CCGATTTTGC CTTCATCCTG CACGCACTGA ACTACCTTTC CGGCAGAGGC 1051 CGCGCCGCTA TCGTCTCATT CCCCGGCATT TTCTATCGCG GCGGCGCAGA 1101 GCAGAAAATc CGCCAATATC TGGTGGAGGG CAACTATGTG GAAACCGTGA 1151 TTGCCCTTGC GCCCAATCTC TTTTACGGCA CCTGCATCGC CGTCAATATC 1201 CTGGTTTTGT CCAAACACAA AGACAATACC GACATCCAAT TCATCGACGC 1251 AAGCGGCTTC TTTAAAAAAG AAACCAACAA CAACGTCTTA ACCGAAGAAC 1301 ACATTGCCGA AATCGTCAAA CTCTTCGCCG ACAAAGCCGA TGTGCCGCAT 1351 ATCGCCCAAA ACGCCGCCCA GCAAACCGTC AAAGACAACG GCTACAACCT 1401 CGCCGTCAGC AGCTATGTCG AAGCCGAAGA CACCCGCGAG GTCATCGACA 1451 TCAGACAGCT CAACGCCGAA ATCAGCGAAA CCgtcgCcaa AATCGAACGG 1501 CTGCGGCGTG AAATTGACGA AGTGATTGCA GAGATTGAAA CCTAG This corresponds to the amino acid sequence <SEQ ID 1908; ORF 605.ng>: g605.pep 1 MMTEMQQRAQ LHRQIWKIAD EVRGAVDGWD FKQYVLGTLF YRFISENFTD 51 YMQAGDSSID YAAMPDSIIT PEIKDDAVKV KGYFIYPGQL FCNIAAEAHQ 101 NEELNTKLKE IFTAIESSAS GYPSEQGIKG LFDDFDTTSS RLGSTVADKN 151 KRLAAVLKGV AELDFGNFED HRIDLFGDAY EYLISNYAAN AGKSGGEFFT 201 PQSVSKLIAR LAVHGQEKVN KIYDPACGSG SLLLQAKKQF DEHIIEEGFF 251 GQEINHTTYN LARMNMFLHN VNYNKFHIEL GDTLTNPKLK DSKPFDAWS 301 NPPYSIDWIG SDDPTLINDD RFAPAGVLAP KSKADFAFIL HALNYLSGRG 351 RAAIVSFPGI FYRGGAEQKI RQYLVEGNYV ETVIALAPNL FYGTCIAVNI 401 LVLSKHKDNT DIQFIDASGF FKKETNNNVL TEEHIAEIVK LFADKADVPH 451 IAQNAAQQTV KDNGYNLAVS SYVEAEDTRE VIDIRQLNAE ISETVAKIER 501 LRREIDEVIA EIET* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 1909>: m605. seq 1 ATGATGACCG AAATGCAACA ACGCGCCCAA CTGCACCGCC AAATTTGGAA 51 AATTGCCGAC GAAGTACGCG GCGCGGTGGA TGGCTGGGAC TTTAAACAAT 101 ACGTTCTCGG CACACTTTTC TACCGCTTTA TCAGCGAAAA CTTCACCGAC 151 TATATGCAGG CAGGCGACAG CAGTATTGAT TACGCCGCTA TGCCGGACAG 201 CATCATCACG CCCGAAATCA AAGACGATGC CGTCAAAGTT AAAGGCTATT 251 TCATCTACCC CGGCCAGCTT TTTTGCAATA TTGCCGCCGA AGCCCATCAA 301 AACGAAGAGC TCAACACCAA GCTGAAAGAA ATTTTTACCG CGATTGAAAG 351 CTCCGCCTCC GGCTATCCGT CCGAACAGGA CATCAAAGGC CTGTTTGACG 401 ACTTCGACAC CACCAGCAGC CGGCTCGGCA GCACTGTTGC CGACAAGAAC 451 AAACGCCTTG CCGCCGTCCT CAAAGGCGTG GCGGAACTCG ATTTCGGCAA 501 TTTTGAAAAC CACCACATCG ACCTTTTCGG CGATGCCTAC GAATACCTGA 551 TTTCCAACTA CGCTGCCAAC GCAGGCAAAT CCGGCGGCGA ATTTTTCACC 601 CCGCAAAGCG TATCCAAGCT GATTGCGCGG CTGGCGGTGC ACGGACAGGA 651 GAAAGTCAAC AAAATCTACG ACCCAGCTTG CGGCTCGGGC AGTCTGCTCT 701 TGCAGGCGAA AAAACAGTTT GACGAGCACA TCATCGAAGA AGGCTTCTTC 751 GGGCAGGAAA TCAACCACAC CACCTACAAC CTCGCCCGCA TGAACATGTT 801 CCTGCACAAC GTCAATTACA ACCAATTCCA CATCGAATTG GGCGACACAC 851 TGACCAACCC AAAGCTCAAA GACAGCAAAC CCTTTGATGC CATCGTTTCC 901 AATCCGCCTT ATTCCATCAA CTGGATAGGC AGCGACGACC CCACCTTAAT 951 CAACGACGAC CGCTTTGCCC CCGCAGGCGT ACTTGCCCCG AAATCCAAAG 1001 CCGATTTTGC CTTCATCCTG CACGCACTGA ACTACCTTTC CGGCAGAGGC 1051 CGCGCCGCCA TCGTCTCATT CCCCGGCATT TTCTATCGCG GCGGCGCAGA 1101 ACAGAAAATC CGCCAATATC TGGTGGAGGG CAACTACGTG GAAACCGTGA 1151 TTGCCCTTGC GCCCAATCTC TTTTACGGCA CCGGCATCGC CGTCAATATC 1201 CTGGTTTTGT CCAAACACAA AGACAATACC GACATCCAAT TCATCGACGC 1251 AAGCGGCTTC TTTAAAAAAG AAACCAACAA CAACGTCTTA ATCGAAGAAC 1301 ACATTGCTGA AATCGTCAAA CTCTTCGCCG ATAAAGCCGA TGTGCCGCAT 1351 ATCGCCCAAA ACGCTGCCCA GCAAACCGTC AAAGACAACG GCTACAACCT 1401 CGCCGTCAGC AGCTATGTCG AAGCCGAAGA CACACGCGAA ATTATCGACA 1451 TCAAACAGCT CAACGCCGAA ATCGGCGAAA CCGTCGCCAA AATCGAACGG 1501 CTGCGGCGTG AAATTGACGA AGTGATTGCA GAGATTGAAG CATGA This corresponds to the amino acid sequence <SEQ ID 1910; ORF 605>: m605.pep 1 MMTEMQQRAQ LHRQIWKIAD EVRGAVDGWD FKQYVLGTLF YRFISENFTD 51 YMQAGDSSID YAAMPDSIIT PEIKDDAVKV KGYFIYPGQL FCNIAAEAHQ 101 NEELNTKLKE IFTAIESSAS GYPSEQDIKG LFDDFDTTSS RLGSTVADKN 151 KRLAAVLKGV AELDFGNFEN HHIDLFGDAY EYLISNYAAN AGKSGGEFFT 201 PQSVSKLIAR LAVHGQEKVN KIYDPACGSG SLLLQAKKQF DEHIIEEGFF 251 GQEINHTTYN LARMNMFLHN VNYNQFHIEL GDTLTNPKLK DSKPFDAIVS 301 NPPYSINWIG SDDPTLINDD RFAPAGVLAP KSKADFAFIL HALNYLSGRG 351 RAAIVSFPGI FYRGGAEQKI RQYLVEGNYV ETVIALAPNL FYGTGIAVNI 401 LVLSKHKDNT DIQFIDASGF FKKETNNNVL IEEHIAEIVK LFADKADVPH 451 IAQNAAQQTV KDNGYNLAVS SYVEAEDTRE IIDIKQLNAE IGETVAKIER 501 LRREIDEVIA EIEA* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 605 shows 97.9% identity over a 513 aa overlap with a predicted ORF (ORF 605. ng) from N gonorrhoeae: m605/g605 10 20 30 40 50 60 m605.pep MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFISEΝFTDYMQAGDSSID MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g605 MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFISEΝFTDYMQAGDSSID 10 20 30 40 50 60 70 80 90 100 110 120 m605. •Pep YAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCΝIAAEAHQΝEELΝTKLKEIFTAIESSAS MMMMMMMMMMMMMMMIMMMMMMMMMMIMMMM g605 YAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCΝIAAEAHQΝEELΝTKLKEIFTAIESSAS 70 80 90 100 110 120 130 140 150 160 170 180 m605. .pep GYPSEQDIKGLFDDFDTTSSRLGSTVADKΝKRLAAVLKGVAELDFGΝFEΝHHIDLFGDAY MMM MMMMMMMMMMMMIMMMIMMMMIhhlMMIM g605 GYPSEQGIKGLFDDFDTTSSRLGSTVADKΝKRLAAVLKGVAELDFGΝFEDHRIDLFGDAY 130 140 150 160 170 180 190 200 210 220 230 240 m605. ■pep EYLISΝYAAΝAGKSGGEFFTPQSVSKLIARLAVHGQEKVΝKIYDPACGSGSLLLQAKKQF IMMMMMMMMMMMIMMMMMMMMMMMMIMMMIMM g605 EYLISΝYAAΝAGKSGGEFFTPQSVSKLIARLAVHGQEKVΝKIYDPACGSGSLLLQAKKQF 190 200 210 220 230 240 250 260 270 280 290 300 m605.pep DEHIIEEGFFGQEIΝHTTYΝLARMΝMFLHΝVΝYΝQFHIELGDTLTΝPKLKDSKPFDAIVS 11 M I ! 1111 E I M 11 M 11111111 ! 11 i ! 111 = 1111111 ! 1111111111 ! 111 ^ 11 g605 DEHIIEEGFFGQEIΝHTTYΝLARMΝMFLHΝVΝYΝKFHIELGDTLTΝPKLKDSKPFDAWS 250 260 270 280 290 300 310 320 330 340 350 360 m605.pep ΝPPYSIΝWIGSDDPTLIΝDDRFAPAGVLAPKSKADFAFILHALΝYLSGRGRAAIVSFPGI MMMMIMMMMMIMMMMMMMMMMMMIMMMMMMIM g605 ΝPPYSIDWIGSDDPTLIΝDDRFAPAGVLAPKSKADFAFILHALΝYLSGRGRAAIVSFPGI 310 320 330 340 350 360 370 380 390 400 410 420 m605.pep FYRGGAEQKIRQYLVEGΝYVETVIALAPΝLFYGTGIAVΝILVLSKHKDΝTDIQFIDASGF MMMMMMIMIMM II 11 II I II 11 II I I 11 II 11 II II II I II II 11 II I II g605 FYRGGAEQKIRQYLVEGΝYVETVIALAPΝLFYGTCIAVΝILVLSKHKDΝTDIQFIDASGF 370 380 390 400 410 420 430 440 450 460 470 480 m605.pep FKKETΝΝΝVLIEEHIAEIVKLFADKADVPHIAQΝAAQQTVKDΝGYΝLAVSSYVEAEDTRE MMMMMMMMMMMMMMMIMMMMMMMMM g605 FKKETΝΝΝVLTEEHIAEIVKLFADKADVPHIAQΝAAQQTVKDΝGYΝLAVSSYVEAEDTRE 430 440 450 460 470 480 490 500 510 m605.pep IIDIKQLNAEIGETVAKIERLRREIDEVIAEIEAX MMMMMIMMMMMMMMMMM: g605 VIDIRQLNAEISETVAKIERLRREIDEVIAEIETX 490 500 510 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1911>: a605. seq 1 ATGATGACCG AAATACAACA ACGCGCCCAA CTGCACCGCC AAATTTGGAA 51 AATTGCCGAC GAAGTACGCG GCGCGGTGGA TGGCTGGGAC TTCAAACAAT 101 ACGTTCTCGG CACACTTTTC TACCGCTTTA TCAGCGAAAA CTTTACCGAC 151 TATATGCAGG CAGGCGACAG CAGTATTGAT TACGCCGCTA TGCCGGACAG 201 CATCATCACG CCCGAAATCA AAGACGATGC CGTCAAAGTC AAAGGCTATT 251 TCATCTACCC CGGCCAGCTT TTTTGCAATA TTGCCGCCGA AGCCCATCAA 301 AACGAAGAGC TCAACACCAA GCTGAAAGAA ATTTTTACCG CGATTGAAAG 351 CTCCGCCTCC GGCTATCCGT CCGAACAAGA CATTAAAGGC CTGTTTGACG 401 ACTTCGACAC CACCAGCAGC CGGCTCGGCA GCACCGTTGC CGACAAGAAC 451 AAACGCCTTG CCGCCGTCCT AAAAGGCGTG GCGGAACTCG ATTTCGGCAG 501 TTTTGAAGAC CACCACATCG ACCTTTTCGG CGATGCCTAC GAATACCTGA 551 TTTCCAACTA CGCTGCCAAC GCAGGCAAAT CCGGCGGCGA ATTTTTCACC 601 CCGCAAAGCG TATCCAAGCT GATTGCGCGG CTGGCGGTGC ACGGGCAGGA 651 GAAAGTAAAC AAAATCTACG ACCCAGCTTG CGGCTCGGGC AGCCTGCTCT 701 TGCAGGCGAA AAAACAGTTT GACGAGCACA TCATCGAAGA AGGCTTCTTC 751 GGGCAGGAAA TCAACCACAC CACCTACAAC CTCGCCCGCA TGAATATGTT 801 TCTGCACAAC GTCAATTACA ACAAATTCCA CATCGAATTG GGCGACACAC 851 TGACCAATCC CAAACTCAAA GACAGCAAAC CCTTTGATGC CGTCGTTTCC 901 AATCCGCCCT ATTCCATCAA CTGGATAGGC AGCGGCGACC CCACCTTAAT 951 CAACGACGAC CGCTTTGCCC CTGCAGGCGT ACTCGCCCCG AAATCCAAAG 1001 CCGATTTTGC CTTCATTCTG CACGCACTGA ACTACCTTTC CGGCAGAGGC 1051 CGCGCCGCCA TCGTCTCATT CCCCGGCATT TTCTATCGCG GCGGCGCAGA 1101 GCAGAAAATC CGCCAATATC TGGTGGAGGG CAACTACGTG GAAACCGTCA 1151 TCGCCCTTGC GCCCAATCTC TTTTACGGCA CCGGCATCGC CGTCAATATA 1201 CTGGTTTTGT CCAAACACAA AGACAATACC GACATCCAAT TCATCGACGC 1251 AGGCGGCTTC TTTAAAAAAG AAACCAACAA CAACGTCTTA ACCGAAGAAC 1301 ACATTGCCGA AATCGTCAAA CTCTTCGCCG ATAAAGCCGA TGTGCCGCAT 1351 ATCGCCCAAA ACGCCGCCCA GCAAACCGTC AAAGACAACG GCTACAACCT 1401 CGCCGTCAGC AGCTATGTTG AACCCGAAGA CACCCGCGAA ATTATCGACA 1451 TCAAACAGCT TAACGCCGAA ATCAGCGAAA CCGTTGCCAA AATCGAACGG 1501 CTGCGGCGTG AAATTGACGA AGTGATTGCA GAGATTGAAG CATGA This corresponds to the amino acid sequence <SEQ ID 1912; ORF 605.a>: a605.pep 1 MMTEIQQRAQ LHRQIWKIAD EVRGAVDGWD FKQYVLGTLF YRFISENFTD 51 YMQAGDSSID YAAMPDSIIT PEIKDDAVKV KGYFIYPGQL FCNIAAEAHQ 101 NEELNTKLKE IFTAIESSAS GYPSEQDIKG LFDDFDTTSS RLGSTVADKN 151 KRLAAVLKGV AELDFGSFED HHIDLFGDAY EYLISNYAAN AGKSGGEFFT 201 PQSVSKLIAR LAVHGQEKVN KIYDPACGSG SLLLQAKKQF DEHIIEEGFF 251 GQEINHTTYN LARMNMFLHN VNYNKFHIEL GDTLTNPKLK DSKPFDAWS 301 NPPYSINWIG SGDPTLINDD RFAPAGVLAP KSKADFAFIL HALNYLSGRG 351 RAAIVSFPGI FYRGGAEQKI RQYLVEGNYV ETVIALAPNL FYGTGIAVNI 401 LVLSKHKDNT DIQFIDAGGF FKKETNNNVL TEEHIAEIVK LFADKADVPH 451 IAQNAAQQTV KDNGYNLAVS SYVEPEDTRE IIDIKQLNAE ISETVAKIER 501 LRREIDEVIA EIEA* m605/a605 98.1% identity in 514 aa overlap 10 20 30 40 50 60 m605.pep MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFISENFTDYMQAGDSSID MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a605 MMTEI QQ RA Q LHR Q IWKIADEVRGAVDGWDFKQYVLGTLFYRFISENFTDYMQAGDSSID 10 20 30 40 50 60 70 80 90 100 110 120 m605. pep YAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNEELNTKLKEIFTAIESSAS MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a605 YAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNEELNTKLKEIFTAIESSAS 70 80 90 100 110 120 130 140 150 160 170 180 m605.pep GYPSEQDIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGVAELDFGNFENHHIDLFGDAY I I I I I I II II II II I I I II II II I II I I II II II II II II II II II : II : II II II II I I a605 GYPSEQDIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGVAELDFGSFEDHHIDLFGDAY 130 140 150 160 170 180 190 200 210 220 230 240 m605.pep EYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEKVNKIYDPACGSGSLLLQAKKQF MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a605 EYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEKVNKIYDPACGSGSLLLQAKKQF 190 200 210 220 230 240 250 260 270 280 290 300 m605. pep DEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNQFHIELGDTLTNPKLKDSKPFDAIVS II II I I II I II II II II II II II II II II I II II : I I II II II I II II II I II II I I : II a605 DEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKFHIELGDTLTNPKLKDSKPFDAWS 250 260 270 280 290 300 310 320 330 340 350 360 m605. pep NPPYSINWIGSDDPTLINDDRFAPAGVLAPKSKADFAFILHALNYLSGRGRAAIVSFPGI II I I I II II I I II II II II II II II II II II II II II I II II II II I I I I II II II I II a605 NPPYSINWIGSGDPTLINDDRFAPAGVLAPKSKADFAFILHALNYLSGRGRAAIVSFPGI 310 320 330 340 350 360 370 380 390 400 410 420 m605.pep FYRGGAEQKIRQYLVEGNYVETVIALAPNLFYGTGIAVNILVLSKHKDNTDIQFIDASGF MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a605 FYRGGAEQKIRQYLVEGNYVETVIALAPNLFYGTGIAVNILVLSKHKDNTDIQFIDAGGF 370 380 390 400 410 420 430 440 450 460 470 480 m605. pep FKKETNNNVLIEEHIAEIVKLFADKADVPHIAQNAAQQTVKDNGYNLAVSSYVEAEDTRE llllllllll II I I II II II II II II II II II II I II I II II II II II I I I II II I II a605 FKKETNNNVLTEEHIAEIVKLFADKADVPHIAQNAAQQTVKDNGYNLAVSSYVEPEDTRE 430 440 450 460 470 480 490 500 510 m605.pep IIDIKQLNAEIGETVAKIERLRREIDEVIAEIEAX II II I II I II I : II II II II II II II II II II I I I a605 IIDIKQLNAEISETVAKIERLRREIDEVIAEIEAX 490 500 510 The following partial DNA sequence was identified in N.gonorrhoeae <SEQ ID 1913>: g606. seq 1 ATGTCCAAAT TTATCGCCAA ACAATCGGTC GGTGCGGAAG TCATCGACAC 51 GCCGcgCACC GAAGAAGAAG CCTGGCTTCT GAACACTGTC GAAGCCCAAg 101 cgcGGCAATG GAATCTGAAA ACGCCAGAAG TCGCCATCTA CCACTCCCCC 151 GAACCCAATG CCTTTGCCAC GGGCGCATCG AGAAACAGCT CCCTGATCGC 201 CGTCAGCacc ggtttgctcg accaTAtgaC GCGCGACgaa gtggaagccg 251 tgTTGGCGCA CGAAATGGCG CACGTCGGCA ACGGCGACAT GGTTACGCTG 301 ACGCTGAtTC AAGGCGTGGT CAATACCTTT GTCGTGTTCC TGTCGCGCAT 351 TATTGCCAAC CTGATTGCCC GAAACAACGA CGGCAGCCAG TCCCAGGGAA 401 CTTATTTCCT AGTCAGCATG GTATTCCAAA TCCTGTTCGG CTTCCTTGCC 451 AGCCTGATTG TCATGTGGTT CAGCCGCCAA CGCGAATACC GCGCCGAcgc 501 gggCGcggCA AAACTGGTCG GCGCACCGAA AATGATTTCC GCCCTGCAAA 551 GGCTTAAAGG CAACCCGGTC GATTTGCCCG AAGAAATGAA CGCAATGGGC 601 ATCGCCGGAG ATACGCGCGA CTCCCTGCTC AGCACCCACC CTTCGCTGGA 651 CAACCGAATC GCCCGCCTCA AATCGCTTTA A This corresponds to the amino acid sequence <SEQ ID 1914; ORF606.ng>: g606.pep 1 MSKFIAKQSV GAEVIDTPRT EEEAWLLNTV EAQARQWNLK TPEVAIYHSP 51 EPNAFATGAS RNSSLIAVST GLLDHMTRDE VEAVLAHEMA HVGNGDMVTL 101 TLIQGWNTF WFLSRIIAN LIARNNDGSQ SQGTYFLVSM VFQILFGFLA 151 SLIVMWFSRQ REYRADAGAA KLVGAPKMIS ALQRLKGNPV DLPEEMNAMG 201 IAGDTRDSLL STHPSLDNRI ARLKSL* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 1915>: m606. seq 1 ATGTCCAAAT TTATCGCCAA ACAATCGGTC GGCGCGGAAG TTATCGACAC 51 GCCGCGCACC GAAGAAGAAG CCTGGCTTTT GAACACTGTC GAAGCCCAAG 101 CGCGGCAATG GAACCTGAAA ACGCCCGAAG TCGCCATCTA CCACTCCCCC 151 GAACCCAATG CCTTTGCCAC GGGCGCATCG AGAAACAGCT CCCTGATCGC 201 CGTCAGCACC GGTTTGCTCG ACCATATGAC GCGTGACGAA GTGGAAGCCG 251 TATTGGCGCA CGAAATGGCA CACGTCGGCA ACGGCGATAT GGTTACGCTG 301 ACGCTGATTC AAGGCGTGGT CAATACCTTT GTCGTGTTCC TGTCGCGCAT 351 TATTGCCAAC CTGATTGCCC GAAACAACGA CGGCAGCCAG TCCCAGGGAA 401 CTTATTTCCT GGTCAGCATG GTATTCCAAA TCCTGTTCGG CTTCCTTGCC 451 AGCTTAATTG TCATGTGGTT CAGCCGACAA CGCGAATACC GCGCCGATGC 501 GGGCGCGGCA AAACTGGTCG GCGCGCCGAA AATGATTTCC GCCCTGCAAA 551 GGCTCAAAGG CAACCCGGTC GATTTGCCCG AAGAAATGAA CGCAATGGGC 601 ATCGCCGGAG ATACGCGCGA CTCCCTGCTC AGCACCCACC CTTCGCTGGA 651 CAACCGTATC GCCCGCCTCA AATCGCTTTA A This corresponds to the amino acid sequence <SEQ ID 1916; ORF 606>: m606.pep 1 MSKFIAKQSV GAEVIDTPRT EEEAWLLNTV EAQARQWNLK TPEVAIYHSP 51 EPNAFATGAS RNSSLIAVST GLLDHMTRDE VEAVLAHEMA HVGNGDMVTL 101 TLIQGWNTF WFLSRIIAN LIARNNDGSQ SQGTYFLVSM VFQILFGFLA 151 SLIVMWFSRQ REYRADAGAA KLVGAPKMIS ALQRLKGNPV DLPEEMNAMG 201 IAGDTRDSLL STHPSLDNRI ARLKSL* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 606 shows 100.0% identity over a 225 aa overlap with a predicted ORF (ORF 606.ng) from N gonorrhoeae: m606/g606 10 20 30 40 50 60 m606. pep MSKFIAKQSVGAEVIDTPRTEEEAWLLΝTVEAQARQWΝLKTPEVAIYHSPEPΝAFATGAS MMMMMMMMIMMMMIMMMMMMMIMMMIMMMMMM g606 MSKFIAKQSVGAEVIDTPRTEEEAWLLΝTVEAQARQWΝLKTPEVAIYHSPEPΝAFATGAS 10 20 30 40 50 60 70 80 90 100 110 120 m606. pep RΝSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGΝGDMVTLTLIQGWΝTFWFLSRIIAΝ MMMMMMMMMMMMMMMMMMMIMMIMMMIMIMMM g606 RΝSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGΝGDMVTLTLIQGWΝTFWFLSRIIAΝ 70 80 90 100 110 120 130 140 150 160 170 180 m606.pep LIARΝΝDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRADAGAAKLVGAPKMIS IMMMMMMMMMMMMMMMMMMMMMMIMMMMMMM g606 LIARΝΝDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRADAGAAKLVGAPKMIS 130 140 150 160 170 180 190 200 210 220 m606.pep ALQRLKGΝPVDLPEEMΝAMGIAGDTRDSLLSTHPSLDΝRIARLKSLX MMMIMMMMMMMIMMMMMMMIMMMMM g606 ALQRLKGΝPVDLPEEMΝAMGIAGDTRDSLLSTHPSLDΝRIARLKSLX 190 200 210 220 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1917>: a606. seq 1 ATGTCCAAAT TCATCGCCAA ACAATCGGTC GGCGCGGAAG TTATCGACAC 51 GCCGCGCACC GAAGAAGAAG CCTGGCTTTT GAACACTGTC GAAGCCCAAG 101 CGCGGCAATG GAACCTGAAA ACGCCCGAAG TCGCCATCTA CCACTCCCCC 151 GAACCCAATG CCTTTGCCAC GGGCGCATCG AGAAACAGCT CCCTGATCGC 201 CGTCAGCACC GGTTTGCTCG ACCATATGAC GCGTGACGAA GTGGAAGCCG 251 TATTGGCGCA CGAAATGGCA CACGTCGGCA ACGGCGATAT GGTTACGCTG 301 ACGCTGATTC AAGGCGTGGT CAATACCTTT GTCGTGTTCC TGTCGCGCAT 351 TATTGCCAAC CTGATTGCCC GAAACAACGA CGGCAGCCAG TCCCAGGGAA 401 CTTATTTCCT GGTCAGCATG GTATTCCAAA TCCTGTTCGG CTTCCTTGCC 451 AGCTTAATTG TCATGTGGTT CAGCCGACAA CGCGAATACC GCGCCGACGC 501 GGGCGCGGCA AAACTGGTCG GCGCGCCGAA AATGATTTCC GCCCTGCAAA 551 GGCTTAAAGG CAACCCGGTC GATTTGCCCG AAGAAATGAA CGCAATGGGC 601 ATCGCCGGAG ATACGCGCGA CTCCCTGCTC AGCACCCACC CTTCGCTGGA 651 CAACCGAATC GCCCGCCTCA AATCGCTTTA A This corresponds to the amino acid sequence <SEQ ID 1918; ORF 606. a>: a606.pep 1 MSKFIAKQSV GAEVIDTPRT EEEAWLLNTV EAQARQWNLK TPEVAIYHSP 51 EPNAFATGAS RNSSLIAVST GLLDHMTRDE VEAVLAHEMA HVGNGDMVTL 101 TLIQGWNTF WFLSRIIAN LIARNNDGSQ SQGTYFLVSM VFQILFGFLA 151 SLIVMWFSRQ REYRADAGAA KLVGAPKMIS ALQRLKGNPV DLPEEMNAMG 201 IAGDTRDSLL STHPSLDNRI ARLKSL* m606/a606 100.0% identity in 226 aa overlap 10 20 30 40 50 60 m606.pep MSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHSPEPNAFATGAS II II II II II II II II I I II II II I I II I I I II I I II II II II II II I II I I I I II I M I a606 MSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHSPEPNAFATGAS 10 20 30 40 50 60 70 80 90 100 110 120 m606. pep RNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGWNTFWFLSRIIAN M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M a606 RNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGWNTFWFLSRIIAN 70 80 90 100 110 120 130 140 150 160 170 180 m606. pep LIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRADAGAAKLVGAPKMIS M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M a 606 LIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRADAGAAKLVGAPKMIS 130 140 150 160 170 180 190 200 210 220 m606. pep ALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARLKSLX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a606 ALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARLKSLX 190 200 210 220 The following partial DNA sequence was identified in N.gonorrhoeae <SEQ ID 1919>: g607.seq 1 ATGCTGCTCG accTcgaCCG CTTTTCCTtt tccGTCTTCC TGAAAGAAAT 51 CCGCCTGCTG ACCGCCCTTG CCCTGCCCAT GCTGTTGGCG CAGGTCGCGC 101 AGGTGGGCAT CGGTTTCGTC GATACCGTGA TGGCGGGCGG TGCGGGCAAG 151 GAAGATTTGG CGGCGGTGGC TTTGGGCAGC AGCGCGTTTG CCACGGTTTA 201 TATTACCTTT ATGGGCATTA TGGCGGCGCT GAACCCGATG ATTGCCCAGC 251 TTTACGGCGC GGGTAAAACC GgtgAAGCAG GCGAAACGGG GCGGCAGGGG 301 ATTTGGTTCG GGCTGATTTT GGGGATTTTC GGCATGATTT TGATGTGGGC 351 GGCGATTACG CCGTTCCGCA ACTGGCTGAC TTTGAGCGAT TATGTGGAAG 401 gcacAAtggc gcAGTATATG CTGTTCACCA GCTTGGCGAT GCCGGCGGCA 451 ATGGTACACC GCGCACTGCA CGCCTACGCT TCCAGCCTGA ACCGCCCGCG 501 CCTGATTATG TTGGTCAGCT TTGCGGCGTT TGTGTTGAAC GTGCCGCTGA 551 ACTATATTTT CGTTTACGGC AAATTCGGTA TGCCCGCTTT GGGTGGCGCA 601 GGTTGCGGCG TGGCGACAAT GGCGGTGTTT TGGTTCAGCG CGCTGGCATT 651 GTGGATTTAT ATCGCCAAGG AAAAATTCTT CCGCCCGTTC GGACTGACAG 701 CGAAATTCGg caaACCGGat tGGgcGGTGT TCAAACAGAT TtGGAAAATC 751 gGcgcgCCCA TCGGGCTGTC TTATTTTTTG GAAgccaGcg cGTTTTCGTT 801 TATCGTGTTT TTGATTGCGC CTttcggCGA GGATTATGTG GCGGCGCAGC 851 AGGTCGGCAT CAGTTTGTCG GGGATTCTCT ATATGATTCC GCAAAGCGTC 901 GGCTCGGCAG GGACGGTGCG CATCGGCTTT TCGCTTGGGC GGCGCGAATT 951 TTCGCGGGCG CGTTATATTT CAGGAGTGTC GCTGGTGTCG GGCTGGGTGC 1001 TCGCCGTGAT TACCGTGCTT TCCTTGGTAT TATTCCGTTC GCCGCTGGCA 1051 AGCATGTACA ACGATGaTCC GGCAGTTTTA AGCATCGCCT CCACCGTCCT 1101 GCTGTTCGCC GGCCTGTtcc aACCGGCAGA CTTCACCCAA TGTATCGCGT 1151 CCTATGCCCT GCGCGGCTAC AAAGTCACCA AGGTGCCGAT GTTCATCCAC 1201 GCCGCCGCCT TCTGGGGCTG CGGCCTGCTG CCGGGCTATC TGCTCGCCTA 1251 CCGTTTCGAT ATGGGCATTT ACGGCTTCTG GACGGCATTG ATTGCCTCGC 1301 TCACCATCGC AGCCGTCGCC TTGGTGTGGT GCTTGGAAAA ATACAGTATG 1351 GAGTTGGTCA AATCACACAA GGCCGTCTGA This corresponds to the amino acid sequence <SEQ ID 1920; ORF 607.ng>: g607.pep 1 MLLDLDRFSF SVFLKEIRLL TALALPMLLA QVAQVGIGFV DTVMAGGAGK 51 EDLAAVALGS SAFATVYITF MGIMAALNPM IAQLYGAGKT GEAGETGRQG 101 IWFGLILGIF GMILMWAAIT PFRNWLTLSD YVEGTMAQYM LFTSLAMPAA 151 MVHRALHAYA SSLNRPRLIM LVSFAAFVLN VPLNYIFVYG KFGMPALGGA 201 GCGVATMAVF WFSALALWIY IAKEKFFRPF GLTAKFGKPD WAVFKQIWKI 251 GAPIGLSYFL EASAFSFIVF LIAPFGEDYV AAQQVGISLS GILYMIPQSV 301 GSAGTVRIGF SLGRREFSRA RYISGVSLVS GWVLAVITVL SLVLFRSPLA 351 SMYNDDPAVL SIASTVLLFA GLFQPADFTQ CIASYALRGY KVTKVPMFIH 401 AAAFWGCGLL PGYLLAYRFD MGIYGFWTAL IASLTIAAVA LVWCLEKYSM 451 ELVKSHKAV* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 1921>: m607. seq 1 ATGCTGCTCG ACCTCAACCG CTTTTCCTTT CCCGTCTTCC TGAAAGAAGT 51 CCGCCTGCTG ACCACTCTTG CCCTGCCCAT GCTGTTGGCG CAGGTCGCGC 101 AGGTGGGCAT CGGTTTTGTC GATACTGTGA TGGCGGGCGG TGCGGGCAAG 151 GAAGACTTGG CGGCGGTGGC TTTGGGCAGC AGCGCGTTTG CCACGGTTTA 201 TATTACCTTT ATGGGCATTA TGGCGGCGCT GAACCCGATG ATTGCCCAGC 251 TTTACGGCGC GGGTAAAACC GACGAAGTGG GCGAAACGGG GCGGCAGGGG 301 ATTTGGTTCG GGCTGTTTTT GGGCGTGTTC GGCATGGTCT TGATGTGGGC 351 GGCGATTACG CCGTTCCGCA ACTGGCTGAC CTTGAGCGAT TATGTGGAAG 401 GCACGATGGC GCAGTATATG TTGTTCACCA GCTTGGCGAT GCCGGCGGCA 451 ATGGTACACC GCGCGCTGCA CGCCTACACT TCCAGCCTGA ACCGCCCGCG 501 CCTGATTATG TTGGTCAGCT TTGCGGCGTT TGTGTTGAAC GTGCCGCTGA 551 ACTATATTTT CGTTTACGGC AAATTCGGTA TGCCCGCTTT GGGCGGCGCA 601 GGCTGCGGAC TGGCGACGAT GGCGGTGTTT TGGTTCAGCG CGCTGGCATT 651 GTGGATTTAT ATCGCCAAGG AAAATTTCTT CCGCCCATTC GGACTGACGG 701 CGAAATTCGG CAAACCGGAT TGGGCGGTGT TCAAACAGAT TTGGAAAATC 751 GGCGCACCCA TCGGGCTGTC TTATTTTTTG GAAGCCAGCG CGTTTTCGTT 801 TATCGTGTTT TTGATTGCGC CTTTCGGCGA GGATTATGTG GCGGCGCAGC 851 AGGTCGGCAT CAGTTTGTCG GGGATTCTCT ATATGATTCC GCAAAGCGTC 901 GGCTCGGCGG GGACGGTGCG CATCGGCTTT TCGCTTGGGC GGCGCGAATT 951 TTCGCGGGCG CGTTATATTT CGGGCGTGTC ACTGGTGTTA GGATGGATGC 1001 TCGCCGTGAT TACCGTGCTT TCCTTGGTAT TATTCCGTTC GCCGCTGGTA 1051 AGTATGTACA ACAATGATCC GGCGGTTTTA AGCATCGCCG CCACCGTCTT 1101 ACTGTTCGCC GGCTTGTTCC AACCGGCAGA CTTCACCCAA TGTATCGCCT 1151 CCTACGCCTT GCGCGGCTAC AAAGTTACAA AGGTGCCGAT GTTCATCCAC 1201 GCCGCCGCCT TTTGGGGCTG CGGCCTGCTG CCGGGCTATC TGCTCGCCTA 1251 CCGTTTCAAT ATGGGCATTT ACGGCTTCTG GACGGCATTG ATTGCCTCGC 1301 TCACCATCGC CGCCATCGCC TTGGTGTGGT GCTTGGAATT GTGCAGTAGG 1351 GAGATGGTCA GATCGCATAA GGCCGTCTGA This corresponds to the amino acid sequence <SEQ ID 1922; ORF 607>: m607.pep 1 MLLDLNRFSF PVFLKEVRLL TTLALPMLLA QVAQVGIGFV DTVMAGGAGK 51 EDLAAVALGS SAFATVYITF MGIMAALNPM IAQLYGAGKT DEVGETGRQG 101 IWFGLFLGVF GMVLMWAAIT PFRNWLTLSD YVEGTMAQYM LFTSLAMPAA 151 MVHRALHAYT SSLNRPRLIM LVSFAAFVLN VPLNYIFVYG KFGMPALGGA 201 GCGLATMAVF WFSALALWIY IAKENFFRPF GLTAKFGKPD WAVFKQIWKI 251 GAPIGLSYFL EASAFSFIVF LIAPFGEDYV AAQQVGISLS GILYMIPQSV 301 GSAGTVRIGF SLGRREFSRA RYISGVSLVL GWMLAVITVL SLVLFRSPLV 351 SMYNNDPAVL SIAATVLLFA GLFQPADFTQ CIASYALRGY KVTKVPMFIH 401 AAAFWGCGLL PGYLLAYRFN MGIYGFWTAL IASLTIAAIA LVWCLELCSR 451 EMVRSHKAV* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 607 shows 94.8% identity over a 459 aa overlap with a predicted ORF (ORF 607.ng) from N gonorrhoeae: m607/g607 10 20 30 40 50 60 m607.pep MLLDLΝRFSFPVFLKEVRLLTTLALPMLLAQVAQVGIGFVDTVMAGGAGKEDLAAVALGS llll llll 1111 1111 : 11111 M 1111111111111 ! 11111111 II 11 M 111 g607 MLLDLDRFSFSVFLKEIRLLTALALPMLLAQVAQVGIGFVDTVMAGGAGKEDLAAVALGS 10 20 30 40 50 60 70 80 90 100 110 120 m607.pep SAFATVYITFMGIMAALΝPMIAQLYGAGKTDEVGETGRQGIWFGLFLGVFGMVLMWAAIT MMMMMMMMMMMMMMM h II II II I II II I = 11 A 11 = 1 II II II g607 SAFATVYITFMGIMAALΝPMIAQLYGAGKTGEAGETGRQGIWFGLILGIFGMILMWAAIT 70 80 90 100 110 120 130 140 150 160 170 180 m607.pep PFRΝWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYTSSLΝRPRLIMLVSFAAFVLΝ MIMMMMIMMMMMMMMMMMMMhllMMMMIMMIMM g607 PFRΝWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYASSLΝRPRLIMLVSFAAFVLΝ 130 140 150 160 170 180 190 200 210 220 230 240 m607. pep VPLΝYIFVYGKFGMPALGGAGCGLATMAVFWFSALALWIYIAKEΝFFRPFGLTAKFGKPD MMMMMMIMIMMMIMMMMMMMMMMIMMMMIMMIM g607 VPLΝYIFVYGKFGMPALGGAGCGVATMAVFWFSALALWIYIAKEKFFRPFGLTAKFGKPD 190 200 210 220 230 240 250 260 270 280 290 300 m607. pep WAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFGEDYVAAQQVGISLSGILYMIPQSV MMMMMMMMMMMMMMMMIMMMMMMMIMMMMMM g607 WAVFK Q IWKIGAPIGLSYFLEASAFSFIVFLIAPFGEDYVAAQQVGISLSGILYMIPQSV 250 260 270 280 290 300 310 320 330 340 350 360 m607. pep GSAGTVRIGFSLGRREFSRARYISGVSLVLGWMLAVITVLSLVLFRSPLVSMYΝΝDPAVL MMMMMMMMMMMIMMM I h II II II I II II I II 11 = II II = 1 II II g607 GSAGTVRIGFSLGRREFSRARYISGVSLVSGWVLAVITVLSLVLFRSPLASMYΝDDPAVL 310 320 330 340 350 360 370 380 390 400 410 420 m607.pep SIAATVLLFAGLFQPADFTQCIASYALRGYKVTKVPMFIHAAAFWGCGLLPGYLLAYRFΝ MIMMMIMMMMMMMMMMMMMMMMMMMMMMIMM: g607 SIASTVLLFAGLFQPADFTQCIASYALRGYKVTKVPMFIHAAAFWGCGLLPGYLLAYRFD 370 380 390 400 410 420 430 440 450 460 m607.pep MGIYGFWTALIASLTIAAIALVWCLELCSREMVRSHKAVX MMIMMMMMIMMMMM I IMMIMII g607 MGIYGFWTALIASLTIAAVALVWCLEKYSMELVKSHKAVX 430 440 450 460 The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 1923>: a607. seq 1 ATGCTGCTCG ACCTCAACCG CTTTTCCTTT TCCGTCTTCC TGAAAGAAGT 51 CCGCCTGCTG ACCGCTCTTG CCCTGCCCAT GCTGTTGGCG CAGGTCGCGC 101 AGGTGGGCAT CGGTTTTGTC GATACCGTGA TGGCGGGCGG TGCGGGCAAG 151 GAAGACTTGG CGGCGGTGGC TTTGGGCAGC AGCGCGTTTG CCACGGTTTA 201 TATTACCTTT ATGGGCATTA TGGCGGCGCT GAACCCGATG ATTGCCCAGC 251 TTTACGGCGC GGGTAAAACC GACGAAGTGG GCGAAACGGG ACGGCAGGGG 301 ATTTGGTTCG GGCTGTTTTT GGGCGTGTTC GGCATGGTCT TGATGTGGGC 351 GGCGATTACG CCGTTCCGCA ACTGGCTGAC CTTGAGCGAT TATGTGGAAG 401 GCACAATGGC GCAGTATATG CTGTTCACCA GCTTGGCGAT GCCGGCGGCA 451 ATGGTACACC GCGCACTGCA CGCCTACGCC TCCAGCCTGA ACCGCCCGCG 501 CCTGATTATG TTGGTCAGCT TTGCGGCGTT TGTGTTGAAC GTGCCGCTGA 551 ACTATATTTT CGTTTACGGC AAATTCGGTA TGCCCGCTTT GGGCGGCGCA 601 GGCTGCGGAC TGGCGACGAT GGCGGTGTTT TGGTTCAGCG CGCTGGCATT 651 GTGGATTTAT ATCGCCAAGG AAAATTTCTT CCGCCCATTC GGACTGACGG 701 CGAAATTCGG CAAACCGGAT TGGGCGGTGT TCAAACAGAT TTGGAAAATC 751 GGCGCACCCA TCGGGCTGTC TTATTTTTTG GAAGCCAGCG CGTTTTCGTT 801 TATCGTGTTT TTGATTGCGC CTTTCGGCGA GGATTATGTG GCGGCGCAGC 851 AGGTCGGCAT CAGTTTGTCG GGGATTCTCT ATATGATTCC GCAAAGCGTC 901 GGCTCGGCGG GGACGGTGCG CATCGGCTTT TCGCTTGGGC GGCGCGAATT 951 TTCGCGGGCG CGTTATATTT CGGGCGTGTC ACTGGTGTCA GGATGGATGC 1001 TCGCCGTGAT TACCGTGCTT TCCTTGGTAT TATTCCGTTC GCCGCTGGTA 1051 AGTATGTACA ACAATGATCC GGCGGTTTTA AGCATCGCCG CCACCGTCTT 1101 ACTGTTCGCC GGCTTGTTCC AACCGGCAGA CTTCACCCAA TGTATCGCCT 1151 CCTACGCCTT GCGCGGCTAC AAAGTTACAA AGGTGCCGAT GTTCATCCAC 1201 GCCGCCGCCT TTTGGGGCTG CGGTCTGCTG CCGGGCTACC TGCTCGCCTA 1251 CCGTTTCGAT ATGGGCATTT ACGGCTTCTG GACGGCATTG ATTGCCTCGC 1301 TCACCATCGC CGCCATCGCC TTGGTGTGGT GCTTGGAATT GTGCAGTAGG 1351 GAGATGGTCA GATCGCATAA GGCTGTCTGA This corresponds to the amino acid sequence <SEQ ID 1924; ORF 607.a>: a607.pep 1 MLLDLΝRFSF SVFLKEVRLL TALALPMLLA QVAQVGIGFV DTVMAGGAGK 51 EDLAAVALGS SAFATVYITF MGIMAALNPM IAQLYGAGKT DEVGETGRQG 101 IWFGLFLGVF GMVLMWAAIT PFRNWLTLSD YVEGTMAQYM LFTSLAMPAA 151 MVHRALHAYA SSLNRPRLIM LVSFAAFVLN VPLNYIFVYG KFGMPALGGA 201 GCGLATMAVF WFSALALWIY IAKENFFRPF GLTAKFGKPD WAVFKQIWKI 251 GAPIGLSYFL EASAFSFIVF LIAPFGEDYV AAQQVGISLS GILYMIPQSV 301 GSAGTVRIGF SLGRREFSRA RYISGVSLVS GWMLAVITVL SLVLFRSPLV 351 SMYNNDPAVL SIAATVLLFA GLFQPADFTQ CIASYALRGY KVTKVPMFIH 401 AAAFWGCGLL PGYLLAYRFD MGIYGFWTAL IASLTIAAIA LVWCLELCSR 451 EMVRSHKAV* m607/a607 98.9% identity in 459 aa overlap 10 20 30 40 50 60 m607. pep MLLDLNRFSFPVFLKEVRLLTTLALPMLLAQVAQVGIGFVDTVMAGGAGKEDLAAVALGS llllllllll II II II I II I : I II II II I I II II II I I II I I II I II II II I I I II I II a607 MLLDLNRFSFSVFLKEVRLLTALALPMLLAQVAQVGIGFVDTVMAGGAGKEDLAAVALGS 10 20 30 40 50 60 70 80 90 100 110 120 m607. pep SAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIWFGLFLGVFGMVLMWAAIT I II II II II II II II II II II II II I II II I II I II II II I II I I I I II I I I I I I M I I I a607 SAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIWFGLFLGVFGMVLMWAAIT 70 80 90 100 110 120 130 140 150 160 170 180 m607.pep PFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYTSSLNRPRLIMLVSFAAFVLN MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a607 PFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYASSLNRPRLIMLVSFAAFVLN 130 140 150 160 170 180 190 200 210 220 230 240 m607.pep VPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALWIYIAKENFFRPFGLTAKFGKPD II II II II II II I II II II II II II II II II II II II II II I II II II I II II I I I I I I I a607 VPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALWIYIAKENFFRPFGLTAKFGKPD 190 200 210 220 230 240 250 260 270 280 290 300 m607.pep WAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFGEDYVAAQQVGISLSGILYMIPQSV II II II II II II II II II I II II II II II II II II I II II II II II II I I II I I II I II I a607 WAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFGEDYVAAQQVGISLSGILYMIPQSV 250 260 270 280 290 300 310 320 330 340 350 360 m607.pep GSAGTVRIGFSLGRREFSRARYISGVSLVLGWMLAVITVLSLVLFRSPLVSMYNNDPAVL I II II II II II II II II II II I II II I II II II I II II II I I I I I II I I I I I I II I II I a607 GSAGTVRIGFSLGRREFSRARYISGVSLVSGWMLAVITVLSLVLFRSPLVSMYNNDPAVL 310 320 330 340 350 360 370 380 390 400 410 420 m607.pep SIAATVLLFAGLFQPADFTQCIASYALRGYKVTKVPMFIHAAAFWGCGLLPGYLLAYRFN MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a607 SIAATVLLFAGLFQPADFTQCIASYALRGYKVTKVPMFIHAAAFWGCGLLPGYLLAYRFD 370 380 390 400 410 420 430 440 450 460 m607.pep MGIYGFWTALIASLTIAAIALVWCLELCSREMVRSHKAVX II II II II II I II II I II II II II II II II II II II I I I I a607 MGIYGFWTALIASLTIAAIALVWCLELCSREMVRSHKAVX 430 440 450 460 The following partial DNA sequence was identified in N.gonorrhoeae <SEQ ID 1925>: g608. seq 1 ATGTCCGCCC TCCTCCCCAT CATCAACCGC CTGATTCTGC AAAGCCCGGA 51 CAGCCGCTCG GAACTTACCT CCTTTGCAGG CAAAACACTG ACCCTGAACA 101 TTGCCGGGCT GAAACTGGCG GGACGCATCA CAGAAGACGG TTTGCTCTCG 151 GCGGGAAACG GCTTTGCAGA CACCGAAATT ACCTTCCGCA ACAGCGCGAT 201 ACGGAAAATC CTCCAAGGCG GCGAACCCGG GGCTGGCGAC ATCAGGCTCG 251 AAGGCGACCT CATCCTCGGC ATcGCGGTAC TGTCCCTGCT CGGCAGCCTG 301 CGTTCCCGCG CATCGGacgA ATTGGCACGG ATTTTCGGCA CGCAGGCAGg 351 catcggcagc CGTGCCACCG ACATCGGACA CGGCaTCaaa cAAATCGGCA 401 GGAACATCGC CGAACAAATC GGCGGATTTT CCCGCGAACC CGAGTCcgCa 451 aacaccggca acgaagccct tgccgactgc ctCGACGAAA TAAGCAGACT 501 GCGCGACGGC GTGGAACGCC TCAACGAACG CCTCGACAGG CTCGAACGCG 551 ACATTTGGAT AGACTAA This corresponds to the amino acid sequence <SEQ ID 1926; ORF 608.ng>: g608.pep 1 MSALLPIINR LILQSPDSRS ELTSFAGKTL TLNIAGLKLA GRITEDGLLS 51 AGNGFADTEI TFRNSAIRKI LQGGEPGAGD IRLEGDLILG IAVLSLLGSL 101 RSRASDELAR IFGTQAGIGS RATDIGHGIK QIGRNIAEQI GGFSREPESA 151 NTGNEALADC LDEISRLRDG VERLNERLDR LERDIWID* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 1927>: m608. seq 1 ATGTCCGCCC TCCTCCCCAT CATCAACCGC CTGATTCTGC AAAGCCCGGA 51 CAGCCGCTCG GAACTTGCCG CCTTTGCAGG CAAAACACTG ACCCTGAACA 101 TTGCCGGGCT GAAACTGGCG GGACGCATCA CGGAAGACGG TTTGCTCTCG 151 GCGGGAAACG GCTTTGCAGA CACCGAAATT ACCTTCCGCA ACAGCGCGGT 201 ACAGAAAATC CTCCAAGGAG GCGAACCCGG GGCGGGCGAC ATCGGGCTCG 251 AAGGCGACCT CATCCTCGGC ATCGCGGTAC TGTCCCTGCT CGGCAGCCTG 301 CGTTCCCGCG CATCGGACGA ATTGGCACGG ATTTTCGGCA CGCAGGCAGA 351 CATCGGCAGC CGTGCCGCCG ACATCGGACA CGGCATCAAA CAAATCGGCA 401 GGAACATCGC CGAACAAATC GGCGGATTTT CCCGCGAATC CGAGTCCGCA 451 AACATCGGCA ACGAAGCCCT TGCCGACTGC CTCGACGAAA TAAGCAGACT 501 GCGCGACGGC GTGGAACGCC TCAACGAACG CCTCGACCGG CTCGAACGCG 551 ACATTTGGAT AGACTAA This corresponds to the amino acid sequence <SEQ ID 1928; ORF 608>: m608.pep 1 MSALLPIINR LILQSPDSRS ELAAFAGKTL TLNIAGLKLA GRITEDGLLS 51 AGNGFADTEI TFRNSAVQKI LQGGEPGAGD IGLEGDLILG IAVLSLLGSL 101 RSRASDELAR IFGTQADIGS RAADIGHGIK QIGRNIAEQI GGFSRESESA 151 NIGNEALADC LDEISRLRDG VERLNERLDR LERDIWID* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 608 shows 95.2% identity over a 188 aa overlap with a predicted ORF (ORF 608.ng) from N gonorrhoeae: m608/g608 10 20 30 40 50 60 m608.pep MSALLPIIΝRLILQSPDSRSELAAFAGKTLTLΝIAGLKLAGRITEDGLLSAGΝGFADTEI I 11111 M I 1111111111111 = H 111111111111 ! 111 II 11111111111111111 g608 MSALLPIIΝRLILQSPDSRSELTSFAGKTLTLΝIAGLKLAGRITEDGLLSAGΝGFADTEI 10 20 30 40 50 60 70 80 90 100 110 120 m608.pep TFRΝSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRSRASDELARIFGTQADIGS M M I h M M M M M M M I I I I 1 1 I I I I I I I I I I I I I I I I I I I I I I 1 1 1 1 I I I I I g608 TFRΝSAIRKILQGGEPGAGDIRLEGDLILGIAVLSLLGSLRSRASDELARIFGTQAGIGS 70 80 90 100 110 120 130 140 150 160 170 180 m608.pep RAADIGHGIKQIGRΝIAEQIGGFSRESESAΝIGΝEALADCLDEISRLRDGVERLΝERLDR llll II II 11 II 11 II II II II I II II II I II g608 RATDIGHGIKQIGRΝIAEQIGGFSREPESAΝTGΝEALADCLDEISRLRDGVERLΝERLDR 130 140 150 160 170 180 189 m608.pep LERDIWIDX g608 LERDIWIDX The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1929>: a608. seq 1 ATGTCCGCCC TCCTCCCCAT CATCAACCGC CTGATTCTGC AAAGCCCGGA 51 CAGCCGCTCG GAACTTGCCG CCTTCGCAGG CAAAACACTG ACCCTGAACA 101 TTGCCGGGTT GAAACTGGCG GGACGCATCA CGGAAGACGG TTTGCTCTCG 151 GCGGGAAACG GCTTTGCAGA CACCGAAATC ACCTTCCGCA ACAGCGCGGT 201 ACAGAAAATC CTCCAAGGCG GCGAACCCGG GGCGGGCGAC ATCGGGCTCG 251 AAGGCGACCT CATCCTCGGC ATCGCGGTAC TGTCCCTGCT CGGCAGCCTG 301 CGTTCCCGCG CATCGGACGA ATTGGCACGG ATTTTCGGCA CGCAGGCAGA 351 CATCGGCAGC CGTGCCGCCG ACATCGGACA CGGCATCAAA CAAATCGGCA 401 GGAACATCGC CGAACAAATC GGCAGATTTT CCCGCGAACC CGAGTCCGCA 451 AACATCGGCA ACGAAGCCCT TGCCGACTGC CTCGACGAAA TAAGCAGACT 501 GCGCGACGGC GTGGAACGCC TCAACGAACG CCTCGACCGG CTCGAACGCG 551 ACATTTGGAT AGACTAA This corresponds to the amino acid sequence <SEQ ID 1930; ORF 608. a>: a608.pep 1 MSALLPIINR LILQSPDSRS ELAAFAGKTL TLNIAGLKLA GRITEDGLLS 51 AGNGFADTEI TFRNSAVQKI LQGGEPGAGD IGLEGDLILG IAVLSLLGSL 101 RSRASDELAR IFGTQADIGS RAADIGHGIK QIGRNIAEQI GRFSREPESA 151 NIGNEALADC LDEISRLRDG VERLNERLDR LERDIWID* m608/a608 98.9% identity in 188 aa overlap 10 20 30 40 50 60 m608 . pep MΞALLPI INRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRITEDGLLSAGNGFADTEI M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M a608 MSALLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRITEDGLLSAGNGFADTEI 10 20 30 40 50 60 70 80 90 100 110 120 mδOδ.pep TFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRSRASDELARIFGTQADIGS II II II II II II II II I II I II I II II II II II II I II II II I II I I I I II I I II I I I M a608 TFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRSRASDELARIFGTQADIGS 70 80 90 100 110 120 130 140 150 160 170 180 m608.pep RAADIGHGIKQIGRNIAEQIGGFSRESESANIGNEALADCLDEISRLRDGVERLNERLDR II II I I II II II II II II II I llll I II II I II I I II II II I I II I I I I I I II I I I M a608 RAADIGHGIKQIGRNIAEQIGRFSREPESANIGNEALADCLDEISRLRDGVERLNERLDR 130 140 150 160 170 180 189 m608.pep LERDIWIDX lllllllll a608 LERDIWIDX The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1931>: g609.seq 1 ATGGTTGTGG ATAGACTCGA AATTCTCGCT CTCGACGACG AAACTCTTGA 51 TGCGTTTGTC GGCAATCAGC GAAGTAGCGA CATCGCGCAC CATATCTTCC 101 ACGAATTTCG GGTTTTCGTA GGCCTTTTCG GTAACGTATT TTTCATCGGG 151 GCGTTTGAGC AGGCCGTAGA GTTGGCAGCT CGCCTGCGTT TCCACATAAT 201 CGATAACTTC CTCGATACCG ACTTCGGCAT CGGAAGTCAG GCTGACGGTA 251 ACGTGCGAAC GCTGATTATG CGCGCCATAT TGGGAAATTT CTTTGGAACA 301 CGGGCAAAGC GAGGTTACGG GAATCATGAC CTTCATACTG TGGCCGTATG 351 CCCCGTCTTT CATTTCACCC GTGAGGCTGA CATCATAATC CAGtaa This corresponds to the amino acid sequence <SEQ ID 1932; ORF 609.ng>: g609.pep 1 MVVDRLEILA LDDETLDAFV GΝQRSSDIAH HIFHEFRVFV GLFGΝVFFIG 51 AFEQAVELAA RLRFHIIDΝF LDTDFGIGSQ ADGΝVRTLIM RAILGΝFFGT 101 RAKRGYGΝHD LHTVAVCPVF HFTREADIII Q* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1933>: m609.seq 1 ATGGTTGTGG ATAGACTCGA AATTCTCGCT CTCGACGACG AAACTCTTGA 51 TGCGTTTGTC GGCAATCAGC GAAGTAGCGA CATCGCGCAC CATATCTTCC 101 ACGAATTTCG GGTTTTCGTA GGCTTTTTCG GTAACGTATT TTTCATCGGG 151 GCGTTTGAGC AGGCCGTAGA GTTGGCAGCT CGCCTGCGCC TCCACATAAT 201 CGATGACTTC CTCGATACCG ACTTCGGCAT CGGCAGTCAG GCTGACGGTA 251 ACGTGCGAAC GCTGGTTGTG CGCGCCGTAT TGGGAAATTT CTTTGGAACA 301 CGGGCAAAGC GAGGTTACGG GAATCATGAC CTTCATACTG TGGCCGTATG 351 CCCCGTCTTT GATTTCGCCC GTGAGACAGA CATCATAATC CAGTAA This corresponds to the amino acid sequence <SEQ ID 1934; ORF 609>: m609.pep 1 MVVDRLEILA LDDETLDAFV GΝQRSSDIAH HIFHEFRVFV GFFGΝVFFIG 51 AFEQAVELAA RLRLHIIDDF LDTDFGIGSQ ADGΝVRTLVV RAVLGΝFFGT 101 RAKRGYGΝHD LHTVAVCPVF DFARETDIII Q* m609/g609 93.1% identity in 131 aa overlap 10 20 30 40 50 60 m609.pep MVVDRLEILALDDETLDAFVGΝQRSSDIAHHIFHEFRVFVGFFGΝVFFIGAFEQAVELAA I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I II I I I I I l I I I I I I I I I I I I I I I I I g609 MVVDRLEILALDDETLDAFVGΝQRSSDIAHHIFHEFRVFVGLFGΝVFFIGAFEQAVELAA 10 20 30 40 50 60 70 80 90 100 110 120 m609.pep RLRLHIIDDFLDTDFGIGSQADGΝVRTLVVRAVLGΝFFGTRAKRGYGΝHDLHTVAVCPVF I I I : I I I I I I I I I I I I I I I I I I I I I I ::| I : I I I I I II I I I I I I I I I I I I II I I I I I I g609 RLRFHIIDNFLDTDFGIGSQADGNVRTLIMRAILGNFFGTRAKRGYGNHDLHTVAVCPVF 70 80 90 100 110 120 130 m609.pep DFARETDIIIQX I : I I : I I I I I I g609 HFTREADIIIQX 130 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1935>: a609. seq 1 ATGGTTGTGG ATAGACTCGA AATTCTCGCT CTCGACGACG AAACTCTTGA 51 TGCGTTTGTC GGCAATCAGC GAAGTAGCGA CATCGCGCAC CATATCTTCC 101 ACGAATTTCG GGTTTTCGTA GGCTTTTTCG GTAACGTATT TTTCATCGGG 151 GCGTTTGAGC AGGCCGTAGA GTTGGCAGCT CGCCTGCGCC TCCACATAAT 201 CGATGACTTC CTCGATACCG ACTTCGGCAT CGGCAGTCAG GCTGACGGTA 251 ACGTGCGAAC GCTGGTTGTG CGCGCCATAT TGGGAAATTT CTTTGGAACA 301 CGGGCAAAGC GAGGTTACGG GAATCATGAC CTTCATACTG TGGCCGTATG 351 CACCGTCTTT CATTTCGCCC GTGAGGCTGA CATCATAATC CAGTAA This corresponds to the amino acid sequence <SEQ ID 1936; ORF 609. a>: a609.pep 1 MVVDRLEILA LDDETLDAFV GΝQRSSDIAH HIFHEFRVFV GFFGΝVFFIG 51 AFEQAVELAA RLRLHIIDDF LDTDFGIGSQ ADGΝVRTLW RAILGΝFFGT 101 RAKRGYGΝHD LHTVAVCTVF HFAREADIII Q* m609/a609 96.9% identity in 131 aa overlap 10 20 30 40 50 60 m609 . pep MVVDRLEILALDDETLDAFVGΝQRSSDIAHHIFHEFRVFVGFFGΝVFFIGAFEQAVELAA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a609 MWDRLEILALDDETLDAFVGΝQRSSDIAHHIFHEFRVFVGFFGΝVFFIGAFEQAVELAA 10 20 30 40 50 60 70 80 90 100 110 120 m609. pep RLRLHIIDDFLDTDFGIGSQADGΝVRTLWRAVLGΝFFGTRAKRGYGΝHDLHTVAVCPVF I II II II I II II II II II II II II II I II II I : II II II I I II I II II I II I I II I I II a609 RLRLHIIDDFLDTDFGIGSQADGΝVRTLWRAILGΝFFGTRAKRGYGΝHDLHTVAVCTVF 70 80 90 100 110 120 130 m609.pep DFARETDIIIQX I II I : I II II I a609 HFAREADIIIQX 130 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 1937>: g610.seq 1 ATGATTGGAG GGCTTATGCA ATTTCCTTAC CGCAATGTTC CGGCTTCGCG 51 TATGCGCCGT ATGCGCAGGG ATGATTTTTC ACGCCGCCTG ATGCGCGAGC 101 ATATGCTGAC CGCCGATGAT TTGATTTATC CGGTGTTCGT ATTGGAGGGG 151 GCGGCGCGCG AGGAGGATGT GCCTTCTATG CCGGGCGTGA AGCGTCAGAG 201 TTTGGACAGG CTGCTGTTTA CGGCGGAAGA GGCGGTGAAG CTCGGTATTC 251 CGATGTTGGC ACTCTTTCCC GTGGTTACGG CAAACAAAAC CGGGCGTGCG 301 CAGGAGGCGT ACAATCCCGA AGGACTCGTG CCGTCAACTG tccgagccTT 351 GCGCGAGAGG TttcCcgaac tggggattat gacggatgtc gcgctcgAtc 401 cttatacggt gcacGGTCAG GACGGACTGA CGGACgaaaa cggttaCGTG 451 ATGAatgATg aaaCCGTAGA AGTCTTGGTG AAACAGGCTT TATGTCATGC 501 AGAGGCGGGC ACGCAGGTCG TTGCTCCTTC CGATATGATG GACGGGCGTA 551 TCGGCGCCAT CCGCGAGGCT TTGGAGGATG CCGGACATAT CCATACGCGG 601 ATTATGGCAT ATTCCGCCAA ATATGCTTCT GCATTCTACG GCCCTTTCCG 651 TGATGCGGTA GGCAGTTCGG GCAATTTGGG AAAGGCAGAT AAAAAGACCT 701 ATCAGATGGA TCCTGCAAAT ACCGATGAGG CGCTGCATGA AGTGGCGCTC 751 GATATTCAGG AAGGTGCGGA TATGGTGATG GTGAAGCCCG GTTTGCCGTA 801 TTTGGACGTT GTCCGCCGCG TGAAGGACGA GTTCGGCGTA CCGACTTATG 851 CCTATCAGGT TTCGGGCGAA TATGCGATGT TGCAGGCGGC GGTTGCCAAC 901 GGCTGGCTGG ACGGCGGCAA AGTGGTTTTG GAAAGCCTGC TGGCATTCAA 951 ACGTGCGGGT GCGGACGGGA TTTTGACCTA TTACGCCATT GAGGCGGCAA 1001 AGATGCTGAA GCGTTGA This corresponds to the amino acid sequence <SEQ ID 1938; ORF 610.ng>: g610.pep 1 MIGGLMQFPY R RNNVVPPAASSRRMMRRRR MRRDDFSRRL MREHMLTADD LIYPVFVLEG 51 AAREEDVPSM PPGGVVKKRRQQSSLLDDRR LLFTAEEAVK LGIPMLALFP VVTANKTGRA 101 QEAYNPEGLV PSTVRALRER FPELGIMTDV ALDPYTVHGQ DGLTDENGYV 151 MNDETVEVLV KQALCHAEAG TQVVAPSDMM DGRIGAIREA LEDAGHIHTR 201 IMAYSAKYAS AFYGPFRDAV GSSGNLGKAD KKTYQMDPAN TDEALHEVAL 251 DIQEGADMVM VKPGLPYLDV VRRVKDEFGV PTYAYQVSGE YAMLQAAVAN 301 GWLDGGKVVL ESLLAFKRAG ADGILTYYAI EAAKMLKR* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1939>: 0. seq 1 ATGATTGGAG GGCTTATGCA GTTTCCTTAC CGCAATGTTC CGGCTTCGCG 51 TATGCGCCGT ATGCGCAGGG ACGATTTTTC ACGCCGCCTG ATGCGCGAAC 101 ACACGCTGAC CGCCGATGAT TTGATTTATC CGGTGTTCGT ATTGGAGGGG 151 TCGGCGCGCG AGGAGGATGT GCCTTCTATG CCGGGTGTGA AGCGTCAAAG 201 TTTGGACAGG CTGCTGTTTA CGGCGGAAGA GGCGGTAAAG CTCGGTATTC 251 CGATGTTGGC ACTGTTCCCC GTGGTTACGG CAAACAAAAC CGAGCGTGCG 301 CAGGAGGCGT ACAATCCCGA AGGACTCGTG CCGTCAACTG TCCGCGCCTT 351 GCGCGAGAGG TTTCCCGAAC TGGGCATTAT GACGGATGTC GCGCTCGATC 401 CTTATACGGT TCACGGTCAG GACGGGCTGA CGGACGAAAA CGGTTATGTG 451 ATGAACGATG AAACCGTAGA GGTTTTGGTC AAGCAGGCTT TGTGCCACGC 501 TGAAGCGGGC GCGCAGGTGG TTGCCCCTTC CGATATGATG GACGGGCGTA 551 TCGGTGCGAT TCGCGAGGCG TTGGAGGATG CCGGGCATAT CCATACGCGG 601 ATTATGGCGT ATTCCGCCAA ATATGCTTCT GCATTTTACG GCCCTTTCCG 651 TGATGCGGTA GGCAGTTCGG GCAATTTGGG CAAGGCAGAT AAAAAGACCT 701 ACCAGATGGA TCCGGCAAAT ACCGATGAGG CGTTGCACGA AGTGGCGTTG 751 GACATTCAGG AAGGTGCGGA TATGGTAATG GTCAAGCCCG GTTTGCCGTA 801 TTTGGACGTT GTCCGCCGCG TAAAGGACGA GTTCGGTGTG CCGACTTATG 851 CCTATCAGGT TTCGGGAGAA TACGCGATGT TGCAGGCAGC GATTGCCAAC 901 GGCTGGCTGG ACGGCGGCAA AGTGGTTTTG GAAAGCCTGC TGGCATTCAA 951 ACGTGCGGGT GCGGACGGGA TTTTGACCTA TTACGCTATT GAGGCGGCAA 1001 AGATGTTGAA GCGTTGA This corresponds to the amino acid sequence <SEQ ID 1940; ORF 610>: m610.pep 1 MIGGLMQFPY RNVPASRMRR MRRDDFSRRL MREHTLTADD LIYPVFVLEG 51 SAREEDVPSM PGVKRQSLDR LLFTAEEAVK LGIPMLALFP VVTANKTERA 101 QEAYNPEGLV PSTVRALRER FPELGIMTDV ALDPYTVHGQ DGLTDENGYV 151 MNDETVEVLV KQALCHAEAG AQVVAPSDMM DGRIGAIREA LEDAGHIHTR 201 IMAYSAKYAS AFYGPFRDAV GSSGNLGKAD KKTYQMDPAN TDEALHEVAL 251 DIQEGADMVM VKPGLPYLDV VRRVKDEFGV PTYAYQVSGE YAMLQAAIAN 301 GWLDGGKVVL ESLLAFKRAG ADGILTYYAI EAAKMLKR* m610/g610 98.5% identity in 338 aa overlap 10 20 30 40 50 60 m610.pep MIGGLMQFPYRNVPAΞRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLEGSAREEDVPSM I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I g610 MIGGLMQFPYRNVPASRMRRMRRDDFSRRLMREHMLTADDLIYPVFVLEGAAREEDVPSM 10 20 30 40 50 60 70 80 90 100 110 120 mδlO.pep PGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTERAQEAYNPEGLVPSTVRALRER I I I I I I I I I I I I I I I I I I I I I I I II I II I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I g610 PGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTGRAQEAYNPEGLVPSTVRALRER 70 80 90 100 110 120 130 140 150 160 170 180 mδlO.pep FPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALCHAEAGAQVVAPSDMM II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I g610 FPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALCHAEAGTQVVAPSDMM 130 140 150 160 170 180 190 200 210 220 230 240 m610.pep DGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSSGNLGKADKKTYQMDPAN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I g610 DGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSSGNLGKADKKTYQMDPAN 190 200 210 220 230 240 250 260 270 280 290 300 m610.pep TDEALHEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVPTYAYQVSGEYAMLQAAIAN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I :| I g610 TDEALHEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVPTYAYQVSGEYAMLQAAVAN 250 260 270 280 290 300 310 320 330 339 m610.pep GWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKRX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g610 GWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKRX 310 320 330 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1941>: a610. seq 1 ATGATTGGAG GGCTTATGCA GTTTCCTTAC CGCAATGTTT CGGCTTCGCG 51 TATGCGCCGT ATGCGCAGGG ACGATTTTTC ACGCCGCCTG ATGCGCGAGC 101 ATACGCTGAC TGCCGATGAT TTGATTTATC CGGTGTTCGT ATTGGAGGGG 151 TCGGCGCGCG AGGAGGATGT GCCTTCTATG CCGGGCGTGA AGCGTCAGAG 201 TTTGGACAGG CTGCTGTTTA CGGCGGAAGA GGCGGTAAAG CTCGGTATTC 251 CGATGTTGGC ACTGTTCCCC GTGGTTACGG CAAACAAAAC CGAGCGTGCG 301 CAGGAGGCGT ACAATCCCGA AGGACTCGTG CCGTCAACTG TCCGCGCCTT 351 GCGCGAGAGG TTTCCCGAAC TGGGCATTAT GACGGATGTC GCGCTCGATC 401 CTTATACGGT GCACGGTCAG GACGGGCTGA CGGACGAAAA CGGTTATGTG 451 ATGAACGATG AAACCGTAGA GGTTTTGGTC AAGCAGGCTT TGTGTCATGC 501 AGAGGCAGGC GCACAGGTCG TTGCTCCTTC CGATATGATG GATGGGCGTA 551 TCGGTGCGAT TCGCGAGGCG TTGGAGGATG CCGGGCATAT CCATACGCGG 601 ATTATGGCGT ATTCCGCCAA ATATGCTTCT GCATTTTACG GCCCTTTCCG 651 TGATGCGGTA GGCAGTTCGG GCAATTTGGG CAAGGCAGAT AAAAAGACCT 701 ACCAGATGGA TCCGGCAAAT ACCGATGAGG CGTTGCACGA AGTGGCGTTG 751 GACATTCAGG AAGGTGCGGA TATGGTGATG GTCAAGCCCG GTTTGCCGTA 801 TTTGGACGTT GTCCGCCGCG TGAAGGACGA GTTCGGCGTG CCGACTTATG 851 CCTATCAGGT TTCGGGAGAA TACGCGATGC TGCAGGCGGC GGTTGCCAAC 901 GGCTGGCTGG ACGGCGGCAA AGTGGTTTTG GAAAGCCTGC TGGCATTCAA 951 ACGTGCGGGT GCGGATGGGA TTTTGACCTA TTACGCCATT GAGGCGGCAA 1001 AGATGCTGAA GCGTTGA This corresponds to the amino acid sequence <SEQ ID 1942; ORF 610. a>: a610.pep 1 MIGGLMQFPY RΝVSASRMRR MRRDDFSRRL MREHTLTADD LIYPVFVLEG 51 SAREEDVPSM PGVKRQSLDR LLFTAEEAVK LGIPMLALFP WTAΝKTERA 101 QEAYNPEGLV PSTVRALRER FPELGIMTDV ALDPYTVHGQ DGLTDENGYV 151 MNDETVEVLV KQALCHAEAG AQWAPSDMM DGRIGAIREA LEDAGHIHTR 201 IMAYSAKYAS AFYGPFRDAV GSSGNLGKAD KKTYQMDPAN TDEALHEVAL 251 DIQEGADMVM VKPGLPYLDV VRRVKDEFGV PTYAYQVSGE YAMLQAAVAN 301 GWLDGGKVVL ESLLAFKRAG ADGILTYYAI EAAKMLKR* m610/a610 99.4% identity in 338 aa overlap 10 20 30 40 50 60 δlO.pep MIGGLMQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLEGSAREEDVPSM lllllllllllll II I II II II II II II II II II II II II II I I I II II II II I I I I II a610 MIGGLMQFPYRNVSASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLEGSAREEDVPSM 10 20 30 40 50 60 70 80 90 100 110 120 m610. pep PGVKRQSLDRLLFTAEEAVKLGIPMLALFPWTANKTERAQEAYNPEGLVPSTVRALRER II II II I II II II II II I II II II II II II I II I II II II I II I I I I II II I I I M I I I I a610 PGVKRQSLDRLLFTAEEAVKLGIPMLALFPWTANKTERAQEAYNPEGLVPSTVRALRER 70 80 90 100 110 120 130 140 150 160 170 180 m610. pep FPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALCHAEAGAQWAPSDMM II II II II II II II II II II II I I II II II II I II I II II II I I II I I II I I II M I M I a610 FPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALCHAEAGAQWAPSDMM 130 140 150 160 170 180 190 200 210 220 230 240 mδlO.pep DGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSSGNLGKADKKTYQMDPAN II II II I I II I II II II II II II II I I II I II I II I I I I I II I I I I I I I I I I I M I I M I a610 DGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSSGNLGKADKKTYQMDPAN 190 200 210 220 230 240 250 260 270 280 290 300 mδlO.pep TDEALHEVALDIQEGADMVMVKPGLPYLDWRRVKDEFGVPTYAYQVSGEYAMLQAAIAN MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a610 TDEALHEVALDIQEGADMVMVKPGLPYLDWRRVKDEFGVPTYAYQVSGEYAMLQAAVAN 250 260 270 280 290 300 310 320 330 339 m610.pep GWLDGGKWLESLLAFKRAGADGILTYYAIEAAKMLKRX II II II II II II I II I I I II II I I II II II II II I II I I a610 GWLDGGKWLESLLAFKRAGADGILTYYAIEAAKMLKRX 310 320 330 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 1943>: gδll.seq 1 ATGCCGTCTG AAAACGGGAT GGGAAAACGG CAGCTTGCGG GCTGCCGTTT 51 GTTCGGGAAG TTAAGCCTTG TTTTCAGGCT GCTGCCCGGA CTCTGTCGAG 101 GCGGTGTCTG CCGGGGCAGG TGCTTCGGTT TTTTCCCGAG TCGGAGCGTG 151 CGGCGCGTTA TCTTCCGCCG CGTCCGCATT Ctcgcgcagg ttgtGGCtgt 201 tatcctTGGG CGGGCTGggt tgtttgcccg ccataaTTtc cagtacctgA 251 TcgcgGTCta tggtttcCCa ttCcatcagg gctttgcaca TCGTTTCCAT 301 cttgTCGCGG TTTTcatcga ggaTTTTGTA ggcaacCTGA TACTgctcgt 351 ccaaaAtccg Gcggatttcc gcgtcgAtgt cctgctgggt tTTCTCGGAA 401 ATGTTTTGCG AACGGgttac gctGCGCCCC AAGAAGACTT CGCCTTCGTT 451 TTCCGCATAA ACCATCACGC CCATTTTGtc gCTCAtgcCG TAGCGCGTTA 501 CCATTTCGCG TGCCATTTGG GTTGCGCGTT CAAAGTCGTT TGA This corresponds to the amino acid sequence <SEQ ID 1944; ORF 61 l.ng>: gδll.pep 1 MPSEΝGMGKR QLAGCRLFGK LSLVFRLLPG LCRGGVCRGR CFGFFPSRSV 51 RRVIFRRVRI LAQVVAVILG RAGLFARHΝF QYLIAVYGFP FHQGFAHRFH 101 LVAVFIEDFV GΝLILLVQΝP ADFRVDVLLG FLGΝVLRTGY AAPQEDFAFV 151 FRIΝHHAHFV AHAVARYHFA CHLGCAFKVV * The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1945>: 611. seq 1 ATGCCGTCTG AAAACGGGAT GGGAAAACGG CAGCTTGCGG GCTGCCGTTT 51 GTTCGGGAAG TTAAGCCTTG TTTTCAGGCT GCTGCTCGGA CTCTGTCGAA 101 GCGGTGTCTG CCGGGGCAGG TGCTTCGGTT TCTTCCCGAG TCGGAGCGTG 151 CGGCGCGTTA TCTTCCGCCG CGTCCGCATT CTCGCGCAGG TTGTGGCTGT 201 AATCTTTGGG CGGGCTGGGT TGTTTGCCCG CCATGATTTC CAGTACCTGA 251 TCGCGGTCGA TGGTTTCCCA TTCCATCAGG GCTTTGCACA TCGTTTCCAT 301 CTTGTCGCGG TTTTCATCGA GGATTTTGTA GGCAACCTGA TATTGCTCGT 351 CCAAAATCCG GCGGATTTCC GCGTCGATGT CCTGCTGGGT TTTCTCGGAA 401 ATGTTTTGCG AACGGGTTAC GCTGCGTCCC AAGAAGACTT CGCCTTCGTT 451 TTCCGCATAA ACCATCACGC CCATTTTGTC GCTCATGCCG TAGCGCGTTA 501 CCATTTCGCG CGCCATTTGG GTTGCGCGTT CAAAGTCGTT TGA This corresponds to the amino acid sequence <SEQ ID 1946; ORF 611>: mδll.pep 1 MPSEΝGMGKR QLAGCRLFGK LSLVFRLLLG LCRSGVCRGR CFGFFPSRSV 51 RRVIFRRVRI LAQVVAVIFG RAGLFARHDF QYLIAVDGFP FHQGFAHRFH 101 LVAVFIEDFV GΝLILLVQΝP ADFRVDVLLG FLGΝVLRTGY AASQEDFAFV 151 FRIΝHHAHFV AHAVARYHFA RHLGCAFKVV * 611/g611 96.1% identity m 180 aa overlap 10 20 30 40 50 60 m611.pep MPSENGMGKRQLAGCRLFGKLSLVFRLLLGLCRSGVCRGRCFGFFPSRSVRRVIFRRVRI I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I :| I I I I I I I I I I I I I I I I I I I I I I I I I g611 MPSENGMGKRQLAGCRLFGKLSLVFRLLPGLCRGGVCRGRCFGFFPSRSVRRVIFRRVRI 10 20 30 40 50 60 70 80 90 100 110 120 mδll.pep LAQVVAVIFGRAGLFARHDFQYLIAVDGFPFHQGFAHRFHLVAVFIEDFVGNLILLVQNP I I I I I I I I : I I I I I I I I I :| I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g611 LAQVVAVILGRAGLFARHNFQYLIAVYGFPFHQGFAHRFHLVAVFIEDFVGNLILLVQNP 70 80 90 100 110 120 130 140 150 160 170 180 m611.pep ADFRVDVLLGFLGNVLRTGYAASQEDFAFVFRINHHAHFVAHAVARYHFARHLGCAFKVV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I lllllllll gβll ADFRVDVLLGFLGNVLRTGYAAPQEDFAFVFRINHHAHFVAHAVARYHFACHLGCAFKVV 130 140 150 160 170 180 m611.pep X I g611 X The following partial DNA sequence was identified in N meningitidis <SEQ ID 1947>: a611. seq 1 ATGCCGTCTG AAAACAGGAT GGGAAAACGG CAGCTTGCGG GCTGCCGTTT 51 GTTCGGGAAG TTAAGCCTTG TTTTCAGGCT GCTGCTCGGA CTCTGTCGAA 101 GCGGTGTCTG CCGGGGCAGG TGCTTCGGTT TCTTCCCGAG TCGGAGCGTG 151 CGGCGCGTTA TCTTCCGCCG CGTCCGCATT CTCGCGCAGG TTGTGGCTGT 201 AATCTTTGGG CGGGCTGGGT TGTTTGCCCG CCATGATTTC CAGTACCTGA 251 TCGCGGTCGA TGGTTTCCCA TTCCATCAGG GCTTTGCACA TCGTTTCCAT 301 CTTGTCGCGG TTTTCATCGA GGATTTTGTA GGCAACCTGA TACTGCTCGT 351 CCAAAATCCG GCGGATTTCC GCATCGATGT CCTGCTGGGT TTTCTCGGAA 401 ATGTTTTGCG AACGGGTTAC GCTGCGTCCC AAGAAGACTT CGCCTTCGTT 451 TTCCGCATAA ACCATCACGC CCATTTTGTC GCTCATGCCG TAGCGCGTTA 501 CCATTTCGCG CGCCATTTGG GTTGCGCGTT CAAAGTCGTT TGA This corresponds to the amino acid sequence <SEQ ID 1948; ORF 61 l.a>: a611.pep 1 MPSEΝRMGKR QLAGCRLFGK LSLVFRLLLG LCRSGVCRGR CFGFFPSRSV 51 RRVIFRRVRI LAQWAVIFG RAGLFARHDF QYLIAVDGFP FHQGFAHRFH 101 LVAVFIEDFV GΝLILLVQΝP ADFRIDVLLG FLGΝVLRTGY AASQEDFAFV 151 FRIΝHHAHFV AHAVARYHFA RHLGCAFKW * m611/a611 98.9% identity in 180 aa overlap 10 20 30 40 50 60 m611. pep MPSEΝGMGKRQLAGCRLFGKLSLVFRLLLGLCRSGVCRGRCFGFFPSRSVRRVIFRRVRI I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a611 MPSEΝRMGKRQLAGCRLFGKLSLVFRLLLGLCRSGVCRGRCFGFFPSRSVRRVIFRRVRI 10 20 30 40 50 60 70 80 90 100 110 120 m611. pep LAQWAVIFGRAGLFARHDFQYLIAVDGFPFHQGFAHRFHLVAVFIEDFVGΝLILLVQΝP M MM M MMM MM M M M M M M M MMM M MM M M M M M M M M a611 LAQWAVIFGRAGLFARHDFQYLIAVDGFPFHQGFAHRFHLVAVFIEDFVGΝLILLVQΝP 70 80 90 100 110 120 130 140 150 160 170 180 m611. pep ADFRVDVLLGFLGΝVLRTGYAASQEDFAFVFRIΝHHAHFVAHAVARYHFARHLGCAFKW II IM II II II I II II I II II II II II II I II I I II II II I I I II II I I I M I I I I I I I I a611 ADFRIDVLLGFLGΝVLRTGYAASQEDFAFVFRIΝHHAHFVAHAVARYHFARHLGCAFKVV 130 140 150 160 170 180 m611.pep X I a 611 X The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1949>: g612.seq 1 ATGGgcttcg gcggcaatat tgcAAAAAAG CTGGCcggGg taGATGAAAT 51 AGCCTttgac tttgacggcA TCGTCTTTGA TTTCGGGCGT GATGATGCTG 101 TCCGGCataG CGGCGTAATC AATGCTGCTG TCGCCGGCCT GCATATAGTC 151 GGTGAAGTTT TCGCTGATAA AGCGGTAGAA AAGTGTGCCG AGAACGTATT 201 GTTTAAAGTC CCAGCCATCC ACCGCGCCGC GTACTTCGTC GGCGATTTTC 251 CAAATTTGGC GGTGCAGTTG GGCGCGTTGT TGCATTTCGG TCATCATCGA 301 AATCCATATA TAAAGTTAAA CAAATCAAAA TCGCCTGATA TTTTCAGACG 351 ATTTTTTTAC GGGCATTCAA ATTAA This corresponds to the amino acid sequence <SEQ ID 1950; ORF 612.ng >: • g612.pep 1 MGFGGΝIAKK LAGVDEIAFD FDGIVFDFGR DDAVRHSGVI ΝAAVAGLHIV 51 GEVFADKAVE KCAEΝVLFKV PAIHRAAYFV GDFPΝLAVQL GALLHFGHHR 101 ΝPYIKLΝKSK SPDIFRRFFY GHSΝ* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1951>: m612. seq 1 ATGGGCTTCG GCGGCAATAT TGCAAAAAAG CTGGCCGGGG TAGATGAAAT 51 AGCCTTTAAC TTTGACGGCA TCGTCTTTGA TTTCGGGCGT GATGATGCTG 101 TCCGGCATAG CGGCGTAATC AATACTGCTG TCGCCTGCCT GCATATAGTC 151 GGTGAAGTTT TCGCTGATAA AGCGGTAGAA AAGTGTGCCG AGAACGTATT 201 GTTTAAAGTC CCAGCCATCC ACCGCGCCGC GTACTTCGTC GGCAATTTTC 251 CAAATTTGGC GGTGCAGTTG GGCGCGTTGT TGCATTTCGG TCATCATCGA 301 AATCCATATA .AAAGTTAAA CAAATCAAAA TCGCCTGATA TTTTCAGACG 351 ATTTTTTTAC GGGCATTCAA ATTAA This corresponds to the amino acid sequence <SEQ ID 1952; ORF 612>: m612.pep 1 MGFGGΝIAKK LAGVDEIAFΝ FDGIVFDFGR DDAVRHSGVI ΝTAVACLHIV 51 GEVFADKAVE KCAEΝVLFKV PAIHRAAYFV GΝFPΝLAVQL GALLHFGHHR 101 ΝPYXKLΝKSK SPDIFRRFFY GHΞΝ* m612/g612 96.0% identity in 124 aa overlap 10 20 30 40 50 60 m612.pep MGFGGΝIAKKLAGVDEIAFΝFDGIVFDFGRDDAVRHSGVIΝTAVACLHIVGEVFADKAVE I I I I I I I I I I I I I I I I I I I :l I I I I I I I I I I I I I I I I I I I I : I I I l llll g612 MGFGGΝIAKKLAGVDEIAFDFDGIVFDFGRDDAVRHSGVIΝAAVAGLHIVGEVFADKAVE 10 20 30 40 50 60 70 80 90 100 110 120 m612.pep KCAEΝVLFKVPAIHRAAYFVGΝFPΝLAVQLGALLHFGHHRΝPYXKLΝKSKSPDIFRRFFY I I I I I I I I I I I I I I I I I I I I I :| I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g612 KCAEΝVLFKVPAIHRAAYFVGDFPΝLAVQLGALLHFGHHRΝPYIKLΝKSKSPDIFRRFFY 70 80 90 100 110 120 m612 . pep GHSΝX M i l l g612 GHSΝX The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1953>: a612.seq 1 ATGGGCTTCG GCGGCAATAT TGCAAAAAAG CTGGCCGGGG TAGATGAAAT 51 AGCCTTTGAC TTTGACGGCA TCGTCTTTGA TTTCGGGCGT GATGATGCTG 101 TCCGGCATAG CGGCGTAATC AATACTGCTG TCGCCTGCCT GCATATAGTC 151 GGTAAAGTTT TCGCTGATAA AGCGGTAGAA AAGTGTGCCG AGAACGTATT 201 GTTTGAAGTC CCAGCCATCC ACCGCGCCGC GTACTTCGTC GGCAATTTTC 251 CAAATTTGGC GGTGCAGTTG GGCGCGTTGT TGTATTTCGG TCATCATCGA 301 AATCCATAT. AAAAGTTAAA CAAATCAAAA TCGCCTGATA TTTTCAGACG 351 ATTTTTT.AC GGGCATTCAA ATTAA This corresponds to the amino acid sequence <SEQ ID 1954; ORF 612. a>: a612.pep 1 MGFGGNIAKK LAGVDEIAFD FDGIVFDFGR DDAVRHSGVI NTAVACLHIV 51 GKVFADKAVE KCAENVLFEV PAIHRAAYFV GNFPNLAVQL GALLYFGHHR 101 NPYXKLNKSK SPDIFRRFFX GHSN* m612/a612 96.0% identity in 124 aa overlap 10 20 30 40 50 60 m612. pep MGFGGNIAKKLAGVDEIAFNFDGIVFDFGRDDAVRHSGVINTAVACLHIVGEVFADKAVE MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a612 MGFGGNIAKKLAGVDEIAFDFDGIVFDFGRDDAVRHSGVINTAVACLHIVGKVFADKAVE 10 20 30 40 50 60 70 80 90 100 110 120 m612. pep KCAENVLFKVPAIHRAAYFVGNFPNLAVQLGALLHFGHHRNPYXKLNKSKSPDIFRRFFY I I I I I I II : II II II I II II II I II I II II II II M II II II I II I I I I I I I II I I I I I a612 KCAENVLFEVPAIHRAAYFVGNFPNLAVQLGALLYFGHHRNPYXKLNKSKSPDIFRRFFX 70 80 90 100 110 120 m612.pep GHSNX II II I a612 GHSNX The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1955>: g613. seq 1 ATGTCGCGTT CGAGCCTGTC GAGGCGTTCG TTGAGGCGTT CCACGCCGTC 51 GCGCAGTCTG CTTATTTCGT CGaggcagtc ggcaagggct tcgttgccgg 101 tgtttGcgGA CTCGGGTTCG CGGGAAAATC CGCCGATTTG TTCGGCGATG 151 TTCCTGCCGA TTTgtttGAt GCCGTGTCCG ATGTCGGTGG CACGgctgcc 201 gatgcCTGCC TGCGTGCCGA AAATCCGTGC CAATTcgtCC GATGCGCGGG 251 AACGCAGGCT GCCGAGCAGG GACAGTACCG CgATGCCGAG GATGAGGTCG 301 CCTTCGAGCC TGATGTCGCC AGCCCCGGGT TCGCCGCCTT GGAGGATTTT 351 CCGTATCGCG CTGTTGCGGA AGGTAATTTC GGTGTCTGCA AAGCCGTTTC 401 CCGCCGAGAG CAAACCGTCT TCTGTGATGC GTCCCGCCAG TTTCAGCCCG 451 GCAATGTTCA GGGTCAGTGT TTTGCCTGCA AAGGAGGTAA GTTCCGAGCG 501 GCTGTCCGGG CTTTGCAGAA TCAGGCGGTT GATGATGGGG AGGAGGGCGG 551 ACATATTTTC TGATTGGGGC GGAGAATGCC TGTTGTTGCT GTTGCCGCTT 601 ATTTTACAGG CTTAA This corresponds to the amino acid sequence <SEQ ID 1956; ORF 613.ng>: g613.pep 1 MSRSSLSRRS LRRSTPSRSL LISSRQSARA SLPVFADSGS REΝPPICSAM 51 FLPICLMPCP MSVARLPMPA CVPKIRAΝSS DARERRLPSR DSTAMPRMRS 101 PSSLMSPAPG SPPWRIFRIA LLRKVISVSA KPFPAESKPS SVMRPASFSP 151 AMFRVSVLPA KEVSSERLSG LCRIRRLMMG RRADIFSDWG GECLLLLLPL 201 ILQA* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1957>: m613. seq 1 ATGTCGCGTT CGAGCCGGTC GAGGCGTTCG TTGAGGCGTT CCACGCCGTC 51 GCGCAGTCTG CTTATTTCGT CGAGGCAGTC GGCAAGGGCT TCGTTGCCGA 101 TGTTTGCGGA CTCGGATTCG CGGGAAAATC CGCCGATTTG TTCGGCGATG 151 TTCCTGCCGA TTTGTTTGAT GCCGTGTCCG ATGTCGGCGG CACGGCTGCC 201 GATGTCTGCC TGCGTGCCGA AAATCCGTGC CAATTCGTCC GATGCGCGGG 251 AACGCAGGCT GCCGAGCAGG GACAGTACCG CGATGCCGAG GATGAGGTCG 301 CCTTCGAGCC CGATGTCGCC CGCCCCGGGT TCGCCTCCTT GGAGGATTTT 351 CTGTACCGCG CTGTTGCGGA AGGTAATTTC GGTGTCTGCA AAGCCGTTTC 401 CCGCCGAGAG CAAACCGTCT TCCGTGATGC GTCCCGCCAG TTTCAGCCCG 451 GCAATGTTCA GGGTCAGTGT TTTGCCTGCA AAGGCGGCAA GTTCCGAGCG 501 GCTGTCCGGG CTTTGCAGAA TCAGGCGGTT GATGATGGGG AGGAGGGCGG 551 ACATATTTTC TGATCGGGGC GGAGAATGCC TGTTGTTGCT GTTGCCGCTT 601 ATTTTACAGG CTTAA This corresponds to the amino acid sequence <SEQ ID 1958; ORF 613>: m613.pep 1 MSRSSRSRRS LRRSTPSRSL LISSRQSARA SLPMFADSDS RENPPICSAM 51 FLPICLMPCP MSAARLPMSA CVPKIRANSS DARERRLPSR DSTAMPRMRS 101 PSSPMSPAPG SPPWRIFCTA LLRKVISVSA KPFPAESKPS SVMRPASFSP 151 AMFRVSVLPA KAASSERLSG LCRIRRLMMG RRADIFSDRG GECLLLLLPL 201 ILQA* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m613/g613 94.6% identity in 204 aa overlap 10 20 30 40 50 60 Itι613.pep MSRSSRSRRSLRRSTPSRSLLISSRQSARASLPMFADSDSREΝPPICSAMFLPICLMPCP II II I II II I I II II II II II I II I II I I I I I : I I I I II II II II I II I I I I I I II I I g613 MSRSSLSRRSLRRSTPSRSLLISSRQSARASLPVFADSGSREΝPPICSAMFLPICLMPCP 10 20 30 40 50 60 70 80 90 100 110 120 m613.pep MSAARLPMSACVPKIRAΝSSDARERRLPSRDSTAMPRMRSPSSPMSPAPGSPPWRIFCTA I I : II II I II I II II II II I II I I I II I I II II I I II I I I I I I I I M I I I II II I I g613 MSVARLPMPACVPKIRAΝSSDARERRLPSRDSTAMPRMRSPSSLMSPAPGSPPWRIFRIA 70 80 90 100 110 120 130 140 150 160 170 180 m613. ■pep LLRKVISVSAKPFPAESKPSSVMRPASFSPAMFRVSVLPAKAASSERLSGLCRIRRLMMG II II II II II II I II I II II II I II I II I II II II II II II MMMMMMMMM g613 LLRKVISVSAKPFPAESKPSSVMRPASFSPAMFRVSVLPAKEVSSERLSGLCRIRRLMMG 130 140 150 160 170 180 190 200 m613. .pep RRADIFSDRGGECLLLLLPLILQAX llllllll I I II II I II I II II II g613 RRADIFSDWGGECLLLLLPLILQAX 190 200 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1959>: a613.seq 1 ATGTCGCGTT CGAGCCGGTC GAGGCGTTCG TTGAGGCGTT CCACGCCGTC 51 GCGCAGTCTG CTTATTTCGT CGAGGCAGTC GGCAAGGGCT TCGTTGCCGA 101 TGTTTGCGGA CTCGGGTTCG CGGGAAAATC TGCCGATTTG TTCGGCGATG 151 TTCCTGCCGA TTTGTTTGAT GCCGTGTCCG ATGTCGGCGG CACGGCTGCC 201 GATGTCTGCC TGCGTGCCGA AAATCCGTGC CAATTCGTCC GATGCGCGGG 251 AACGCAGGCT GCCGAGCAGG GACAGTACCG CGATGCCGAG GATGAGGTCG 301 CCTTCGAGCC CGATGTCGCC CGCCCCGGGT TCGCCGCCTT GGAGGATTTT 351 CTGTACCGCG CTGTTGCGGA AGGTGATTTC GGTGTCTGCA AAGCCGTTTC 401 CCGCCGAGAG CAAACCGTCT TCCGTGATGC GTCCCGCCAG TTTCAACCCG 451 GCAATGTTCA GGGTCAGTGT TTTGCCTGCG AAGGCGGCAA GTTCCGAGCG 501 GCTGTCCGGG CTTTGCAGAA TCAGGCGGTT GATGATGGGG AGGAGGGCGG 551 ACATATTTTC TGATCGGGGC GGAGAATGCC TGTTGTTGCT GTTGACGCTT 601 ATTTTACAGG CTTAA This corresponds to the amino acid sequence <SEQ ID 1960; ORF 613.a>: a613.pep 1 MSRSSRSRRS LRRSTPSRSL LISSRQSARA SLPMFADSGS REΝLPICSAM 51 FLPICLMPCP MSAARLPMSA CVPKIRAΝSS DARERRLPSR DSTAMPRMRS 101 PSSPMSPAPG SPPWRIFCTA LLRKVISVSA KPFPAESKPS SVMRPASFΝP 151 AMFRVSVLPA KAASSERLSG LCRIRRLMMG RRADIFSDRG GECLLLLLTL 201 ILQA* m613/a613 98.0% identity in 204 aa overlap 10 20 30 40 50 60 m613. ■pep MSRSSRSRRSLRRSTPSRSLLISSRQSARASLPMFADSDSREΝPPICSAMFLPICLMPCP II II I II II II II I II I II II II II II II I II II II II llll II II II II I I I I I II I a613 MSRSSRSRRSLRRSTPSRSLLISSRQSARASLPMFADSGSREΝLPICSAMFLPICLMPCP 10 20 30 40 50 60 70 80 90 100 110 120 m613. pep MSAARLPMSACVPKIRAΝSSDARERRLPSRDSTAMPRMRSPSSPMSPAPGSPPWRIFCTA I II II II I II II II II II II II I II II II II II II II II II II II II I II II II I II I I I a613 MSAARLPMSACVPKIRAΝSSDARERRLPSRDSTAMPRMRSPSSPMSPAPGSPPWRIFCTA 70 80 90 100 110 120 130 140 150 160 170 180 m613.pep LLRKVISVSAKPFPAESKPSSVMRPASFSPAMFRVSVLPAKAASSERLSGLCRIRRLMMG I II II I II II II II I II II I II II II II : I II I II II II I II II II I I I I II II I II II I a613 LLRKVISVSAKPFPAESKPSSVMRPASFΝPAMFRVSVLPAKAASSERLSGLCRIRRLMMG 130 140 150 160 170 180 190 200 m613.pep RRADIFSDRGGECLLLLLPLILQAX II II II II II II II I II I llllll a613 RRADIFSDRGGECLLLLLTLILQAX 190 200 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 1961>: g614. seq 1 AtggcTgcgt tcAacgcttt ggacggcaaa aaagaagaca acgggcaaat 51 cgaaTATTCT CAGTTCATCC GACAGGTCAA CAACGGCGAA GTATCCGGCG 101 TCAACATCGA AGGATCCGTC GTCAGCGGTT ACCTGATTAA AGGCGAGCGC 151 ACCGACAAAA GCACCTTCTT CACCAACGCG CCCTTGGATG ACAACCTGAT 201 TCAAACCCTT TTGAACAAAA ACGTCCGCGT AAAAGTAACG CCGGAAGAAA 251 AACCGAGCGC GCTGACTGCC CTGTTTTACA GCCTGCTGCC CGTCCTGCTG 301 CTGATTGGCG CATGGTTCTA CTTTATGCGT ATGCAGGCGG GCGGCGGCGG 351 AAAAGGCGGC GCATTCTCCT TCGGCAAAAG CCGCGCCCGC CTGCTGGACA 401 AAGATGCCAA CAAAGTTACC TTTGCCGATG TCGCCGGCTG CGACGAAGCC 451 AAAGAAGAAG TGCAGGAAAT CGTCGATTAC CTCAAAGCAC CGAACCGCta 501 tcaAAGcctc ggcggccgtg ttcCGCGCGG CATCCtgCtg gcgGgcagcc 551 CGGGAaccgg taaAACACTC TTGGCGAAAG CCATTGCAGG CGAGGCCGGC 601 GTGCCGTTCT TCAGCATTTC CGGTTCCGAT TTTGTCGAAA TGTTCGTCGG 651 TGTCGGTGCA AGCCGCGTCC GCGATATGTT CGAGCAGGCA AAGAAAAACG 701 CCCCATGCAT TATCTTTATC GACGAGATTG ACGCGGTAGG CCGCCAACGC 751 GGCGCAGgTT TGGGCGGCGG CAATGATGAG CGCGAGCAAA CATTAAACCA 801 ATTATTGGTT GAAATGGACG GTTTTGAGAG CAATCAGACT GTAATTGTGA 851 TTGCGGCAAC CAACCGCCCC GACGTACTCG ATCCTGCGCT GCAACGCCCC 901 GGCCGCTTCG ACCGCCAAGT CGTCGTCCCC CTGCCGGACA TCCGGGGGCG 951 CGAACAGatn ttGAACGTCC ATTCtaaAAA AGTGCcttTG gacgaATCTg 1001 tggaTTTATT GTCCCTCGCG CGCGGCACGC ccggtttTTc cggcgcggat 1051 tTggcgaaac tggtcaacga agcccccctg tttgccggcc gccgcaacaa 1101 agtgaaagtc gatcaaagcg attTGAAGAC GCCAAAGACA AAATCTATAT 1151 GGGTCCGGAA CGCCGCAGTA TGGTGA This corresponds to the amino acid sequence <SEQ ID 1962; ORF 614.ng>: g614.pep 1 MAAFNALDGK KEDNGQIEYS QFIRQVNNGE VSGVNIEGSV VSGYLIKGER 51 TDKSTFFTNA PLDDNLIQTL LNKNVRVKVT PEEKPSALTA LFYSLLPVLL 101 LIGAWFYFMR MQAGGGGKGG AFSFGKSRAR LLDKDANKVT FADVAGCDEA 151 KEEVQEIVDY LKAPNRYQSL GGRVPRGILL AGSPGTGKTL LAKAIAGEAG 201 VPFFSISGSD FVEMFVGVGA SRVRDMFEQA KKNAPCIIFI DEIDAVGRQR 251 GAGLGGGNDE REQTLNQLLV EMDGFESNQT VIVIAATNRP DVLDPALQRP 301 GRFDRQVWP LPDIRGREQX LNVHSKKVPL DESVDLLSLA RGTPGFSGAD 351 LAKLVNEAPL FAGRRNKVKV DQSDLKTPKT KSIWVRNAAV W* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1963>: m614. seq 1 ATGGCTGCGT TCAACGCTTT AGACGGTAAA AAAGAAGACA ACGGGCAAAT 51 CGAATACTCT CAGTTCATCC AACAGGTCAA CAACGGCGAA GTATCCGGCG 101 TCAACATCGA AGGATCCGTC GTCAGCGGCT ACCTGATTAA GGGCGAGCGC 151 ACCGACAAAA GCACTTTCTT CACCAACGCG CCTTTGGACG ACAACCTAAT 201 TAAAACACTG CTCGACAAAA ACGTCCGCGT AAAAGTAACG CCGGAAGAAA 251 AACCGAGCGC GCTGGCTGCC CTGTTTTACA GCCTGCTGCC CGTCCTGCTG 301 CTGATTGGCG CATGGTTCTA CTTCATGCGT ATGCAGACGG GCGGCGGCGG 351 AAAAGGCGGC GCATTCTCAT TCGGTAAAAG CCGCGCCCGC CTGCTGGACA 401 AAGATGCCAA CAAAGTGACC TTTGCCGATG TCGCCGGCTG CGACGAAGCC 451 AAAGAAGAAG TACAGGAAAT CGTCGATTAC CTCAAAGCGC CGAACCGCTA 501 TCAAAGCCTG GGCGGGCGCG TGCCGCGCGG CATCCTGCTG GCGGGCAGCC 551 CGGGTACGGG TAAGACGCTT TTGGCGAAAG CGATTGCAGG CGAAGCCGGC 601 GTGCCGTTCT TCAGCATTTC AGGTTCCGAC TTTGTCGAAA TGTTCGTCGG 651 TGTCGGTGCG AGCCGCGTCC GCGATATGTT CGAGCAGGCG AAGAAAAACG 701 CCCCCTGCAT CATCTTTATC GACGAGATTG ACGCAGTCGG CCGCCAACGC 751 GGCGCAGGTT TGGGCGGCGG CAATGATGAG CGCGAGCAAA CATTAAACCA 801 ATTGTTGGTT GAAATGGACG GTTTTGAGAG CAATCAGACT GTAATTGTGA 851 TTGCGGCAAC CAACCGCCCC GACGTACTCG ATCCTGCGCT GCAACGCCCC 901 GGCCGTTTCG ACCGCCAAGT GGTTGTCCCC CTGCCGGACA TCCGAGGGCG 951 CGAACAGATT TTGAACGTCC ATTCTAAAAA AGTGCCTTTG GACGAATCTG 1001 TGGATTTATT GTCCCTCGCG CGCGGCACGC CGGGTTTTTC CGGCGCGGAT 1051 TTGGCGAACT TGGTCAACGA AGCCGCCCTG TTTGCCGGCC GCCGCAATAA 1101 AGTCAAAGTC GATCAGAGCG ATTTGAAGAC GCCAAAGACA AAATCTATAT 1151 GGGTCCGGAA CGCCGCAGTA TGGTGA This corresponds to the amino acid sequence <SEQ ID 1964; ORF 614>: m61 .pep 1 MAAFNALDGK KEDNGQIEYS QFIQQVNNGE VSGVNIEGSV VSGYLIKGER 51 TDKSTFFTNA PLDDNLIKTL LDKNVRVKVT PEEKPSALAA LFYSLLPVLL 101 LIGAWFYFMR MQTGGGGKGG AFSFGKSRAR LLDKDANKVT FADVAGCDEA 151 KEEVQEIVDY LKAPNRYQSL GGRVPRGILL AGSPGTGKTL LAKAIAGEAG 201 VPFFSISGSD FVEMFVGVGA SRVRDMFEQA KKNAPCIIFI DEIDAVGRQR 251 GAGLGGGNDE REQTLNQLLV EMDGFESNQT VIVIAATNRP DVLDPALQRP 301 GRFDRQVWP LPDIRGREQI LNVHSKKVPL DESVDLLSLA RGTPGFSGAD 351 LANLVNEAAL FAGRRNKVKV DQSDLKTPKT KSIWVRNAAV W* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N. gonorrhoeae m614/g614 98.0% identity in 391 aa overlap 10 20 30 40 50 60 m614 . pep MAAFΝALDGKKEDΝGQIEYSQFIQQVΝΝGEVSGVΝIEGSWSGYLIKGERTDKSTFFTΝA M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M g614 MAAFΝALDGKKEDΝGQIEYSQFIRQVΝΝGEVSGVΝIEGSWSGYLIKGERTDKSTFFTΝA 10 20 30 40 50 60 70 80 90 100 110 120 m614. pep PLDDΝLIKTLLDKΝVRVKVTPEEKPSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGG MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g614 PLDDΝLIQTLLΝKΝVRVKVTPEEKPSALTALFYSLLPVLLLIGAWFYFMRMQAGGGGKGG 70 80 90 100 110 120 130 140 150 160 170 180 m614.pep AFSFGKSRARLLDKDAΝKVTFADVAGCDEAKEEVQEIVDYLKAPΝRYQSLGGRVPRGILL II I II II II II II II II II II II I II II I II II I II I II II I II I I II II I I I I II I I II g614 AFSFGKSRARLLDKDAΝKVTFADVAGCDEAKEEVQEIVDYLKAPΝRYQSLGGRVPRGILL 130 140 150 160 170 180 190 200 210 220 230 240 m614. pep AGSPGTGKTLLAKAIAGEAGVPFFSISGSDFVEMFVGVGASRVRDMFEQAKKΝAPCIIFI I I I I I II II I II II II II II II I II II II II I II II I I II II II I I I II I I I I I I I I I I I g614 AGSPGTGKTLLAKAIAGEAGVPFFSISGSDFVEMFVGVGASRVRDMFEQAKKΝAPCIIFI 190 200 210 220 230 240 250 260 270 280 290 300 m61 .pep DEIDAVGRQRGAGLGGGΝDEREQTLΝQLLVEMDGFESΝQTVIVIAATΝRPDVLDPALQRP II II II II I II II II II II I II II II I I I I II II I II I II II I I II I I I I I I I I I I I I M g61 DEIDAVGRQRGAGLGGGΝDEREQTLΝQLLVEMDGFESΝQTVIVIAATΝRPDVLDPALQRP 250 260 270 280 290 300 310 320 330 340 350 360 m614.pep GRFDRQVWPLPDIRGREQILΝVHSKKVPLDESVDLLSLARGTPGFSGADLAΝLVΝEAAL II II II II II I I II I II II II II II II II I II I II I II I II II II II II II : II II I I g614 GRFDRQVWPLPDIRGREQXLΝVHSKKVPLDESVDLLSLARGTPGFSGADLAKLVΝEAPL 310 320 330 340 350 360 370 380 390 m614.pep FAGRRΝKVKVDQSDLKTPKTKSIWVRΝAAVWX II II I I I II I II II II II I I II I II I II II II g614 FAGRRΝKVKVDQSDLKTPKTKSIWVRΝAAVWX 370 380 390 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1965>: a614. seq 1 ATGGCTGCGT TCAACGCTTT AGACGGTAAA AAAGAAGACA ACGGGCAAAT 51 CGAATATTCT CAGTTCATCC AACAGGTCAA CAACGGCGAA GTATCCGGCG 101 TCAACATCGA AGGATCCGTC GTCAGCGGCT ACCTGATTAA GGGCGAGCGC 151 ACCGACAAAA GCACCTTCTT CACCAACGCG CCTTTGGACG ACAACCTGAT 201 TAAAACACTG CTCGACAAAA ACGTCCGTGT AAAAGTAACG CCGGAAGAAA 251 AACCGAGCGC GCTGGCTGCC CTGTTTTACA GCCTGCTGCC CGTCCTGCTG 301 CTGATTGGCG CGTGGTTCTA CTTTATGCGT ATGCAGACGG GCGGCGGCGG 351 AAAAGGCGGC GCATTCTCAT TCGGCAAAAG CCGCGCCCGC CTACTGGACA 401 AAGATGCCAA CAAAGTTACC TTTGCCGATG TCGCCGGCTG CGACGAAGCC 451 AAAGAAGAAG TGCAGGAAAT CGTCGATTAC CTCAAAGCGC CGAACCGCTA 501 TCAAAGCCTG GGCGGGCGCG TGCCGCGCGG CATCCTGCTG GCGGGCAGCC 551 CGGGTACGGG TAAGACGCTT TTGGCGAAAG CGATTGCAGG CGAAGCCGGC 601 GTGCCGTTCT TCAGCATTTC AGGTTCCGAC TTTGTCGAAA TGTTCGTCGG 651 TGTCGGTGCA AGCCGCGTCC GCGATATGTT CGAGCAGGCG AAGAAAAACG 701 CCCCCTGCAT CATCTTTATC GACGAGATTG ACGCAGTCGG CCGCCAACGC 751 GGCGCAGGTT TGGGCGGCGG TAATGATGAG CGCGAGCAAA CATTAAACCA 801 ATTGTTGGTT GAAATGGACG GTTTTGAGAG CAATCAGACT GTAATTGTGA 851 TTGCGGCAAC CAACCGCCCC GACGTACTCG ATCCTGCGCT GCAACGCCCC 901 GGCCGTTTCG ACCGCCAAGT GGTTGTCCCC CTGCCGGACA TCCGGGGGCG 951 CGAACAGATT TTGAACGTCC ACTCTAAAAA AGTGCCTTTG GACAAATCTG 1001 TGGATTTATT GTCCCTCGCG CGCGGCACGC CGGGTTTTTC CGGCGCGGAT 1051 TTGGCGAACT TGGTCAACGA AGCCGCCCTG TTTGCCGGCC GCCGCAATAA 1101 AGTCAAAGTC GATCAGAGCG ATTTGAAGAC GCCAAAGACA AAATCTATAT 1151 GGGTCCGGAA CGCCGCAGTA TGGTGA This corresponds to the amino acid sequence <SEQ ID 1966; ORF 614.a>: a61 .pep 1 MAAFNALDGK KEDNGQIEYS QFIQQVNNGE VSGVNIEGSV VSGYLIKGER 51 TDKSTFFTNA PLDDNLIKTL LDKNVRVKVT PEEKPSALAA LFYSLLPVLL 101 LIGAWFYFMR MQTGGGGKGG AFSFGKSRAR LLDKDANKVT FADVAGCDEA 151 KEEVQEIVDY LKAPNRYQSL GGRVPRGILL AGSPGTGKTL LAKAIAGEAG 201 VPFFSISGSD FVEMFVGVGA SRVRDMFEQA KKNAPCIIFI DEIDAVGRQR 251 GAGLGGGNDE REQTLNQLLV EMDGFESNQT VIVIAATNRP DVLDPALQRP 301 GRFDRQVWP LPDIRGREQI LNVHSKKVPL DKSVDLLSLA RGTPGFSGAD 351 LANLVNEAAL FAGRRNKVKV DQSDLKTPKT KSIWVRNAAV W* m614/a614 99.7% identity in 391 aa overlap 10 20 30 40 50 60 m614.pep MAAFNALDGKKEDNGQIEYSQFIQQVNNGEVSGVNIEGSWSGYLIKGERTDKSTFFTNA I I II I II II II I II II I II II II II I II II I II I I II I I I I I II II I I I I II II II I I I I a614 MAAFNALDGKKEDNGQIEYSQFIQQVNNGEVSGVNIEGSWSGYLIKGERTDKSTFFTNA 10 20 30 40 50 60 70 80 90 100 110 120 m614.pep PLDDNLIKTLLDKNVRVKVTPEEKPSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGG I II II II II II I II II II II II I I II II II II I I II II II II II II II I I II II II II I I a614 PLDDNLIKTLLDKNVRVKVTPEEKPSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGG 70 80 90 100 110 120 130 140 150 160 170 180 m614. pep AFSFGKSRARLLDKDANKVTFADVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILL II II II II II II II II II II I II II I II II II II II II II I I II II II I II I I I I II II I a614 AFSFGKSRARLLDKDANKVTFADVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILL 130 140 150 160 170 180 190 200 210 220 230 240 m614.pep AGSPGTGKTLLAKAIAGEAGVPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFI II I I II II II II II II I I I II I I II I I II II I II II II I I II I II II I I II I II I I I I I I a614 AGSPGTGKTLLAKAIAGEAGVPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFI 190 200 210 220 230 240 250 260 270 280 290 300 m614. pep DEIDAVGRQRGAGLGGGNDEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRP II II II I II I II II II II I I I II I I I I I II I II I II II II I I II M I I I I I I M I I M M a614 DEIDAVGRQRGAGLGGGNDEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRP 250 260 270 280 290 300 310 320 330 340 350 360 m614. pep GRFDRQVWPLPDIRGREQILNVHSKKVPLDESVDLLSLARGTPGFSGADLANLVNEAAL MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a614 GRFDRQVWPLPDIRGREQILNVHSKKVPLDKSVDLLSLARGTPGFSGADLANLVNEAAL 310 320 330 340 350 360 370 380 390 m614. pep FAGRRNKVKVDQSDLKTPKTKSIWVRNAAVWX I II II II II II II II II II II I II II II II II a614 FAGRRNKVKVDQSDLKTPKTKSIWVRNAAVWX 370 380 390 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1967>: g615. seq 1 ATGTGGAAAC GGCGGCGGCG CGGTGtcggC AGCTTtgaag agcagcGaAT 51 agatgCCGCC GGCAAACCAC AATGCGGAAa gcaggCtgaa gcGGTTgcgC 101 GGCagcTTca tGCCGCCTCC TcGTCCaGCC ACGtttGgca gattttggac 151 aggcgcAGga ATTTGCcgCc gcgtgcggCA agtatgtcgc gcCAttgtgc 201 cacttcttcg gcggacggTG cttcgtcgaT gctgCATTCG TACagcagga 251 aatcgagggt ttcttcgatg acggGgatgg AttccgTTTG GataAgCTgc 301 ttgagttcgt tcatgactGt TCgGATAcgg aaatcgggaa aatgccgtct 351 gAaagggctt CAGACGGCat tggATTATTT GCTGTGCAGG AAgcgcgttg 401 cctcttccca tttgcCGGAA AtgATGTCGg gtacggcctg cAGGGATttg 451 gCGACGGcat cgtcgatttg ccgGcggtgc ttCcgcgctc ggtttGTTca 501 agacgtagcc gaCGACGagg ttgcggtcGC CGGGGtggcC GATGCCGAGG 551 CGCAGGCGGt aatagtctgC CGTGCCGAGT TTTGCctgAA TGTCTTTCAA 601 GCCGTTGTGT CcgcCGttgc cgcCGCCGAG TTTGAATTTg ATCCGTCCGC 651 AAGGGATGTC GAGTTCGTCG TGGACGACGA GGATTTCTTC GGGTTTGATT 701 TTGTAGAACT GTGCAAGCGC GGCAACCGCC TGTCCGGAAC GGTTCATGAA 751 CGTGGCCGGT TTGAGCAGCC AAACATCGCC GTCGGGCAGG GCGGCGCGGG 801 CAACTTCGCC GAAGAATTTT TTTTCTTCTT TAAACGAAGC CTTCCATTTC 851 CACGCCAGTT CGTCGAGGAA CCAAAAGCCC GCATTGTGGC GGGTCTGTTC 901 GTATTCTTTG CCCGGGTTGC CCAAGCCGAC AACCATTTTG ATTGTGttcg 951 acatgataTT TtccgtgTTT CTgTCGaatg cggtCtgaAG GCTTCAGacg 1001 gcatggTtaT TCTTCTTgaT TTtgaACgcg tgtgcggCGC GCTTCTTTGG 1051 GGTCGATCAA CAGCGGGCGG TACACTTCGA TGCGGTCGCC GTCGCGCAGC 1101 GGCGTGTCGT CTTTGA This corresponds to the amino acid sequence <SEQ ID 1968; ORF 615.ng>: g615.pep 1 MWKRRRRGVG SFEEQRIDAA GKPQCGKQAE AVARQLHAAS SSSHVWQILD 51 RRRΝLPPRAA SMSRHCATSS ADGASSMLHS YSRKSRVSSM TGMDSVWISC 101 LSSFMTVRIR KSGKCRLKGL QTALDYLLCR KRVASSHLPE MMSGTACRDL 151 ATASSICRRC FRARFVQDVA DDEVAVAGVA DAEAQAVIVC RAEFCLΝVFQ 201 AVVSAVAAAE FEFDPSARDV EFWDDEDFF GFDFVELCKR GΝRLSGTVHE 251 RGRFEQPΝIA VGQGGAGΝFA EEFFFFFKRS LPFPRQFVEE PKARIVAGLF 301 VFFARVAQAD ΝHFDCVRHDI FRVSVECGLK ASDGMVILLD FERVCGALLW 351 GRSTAGGTLR CGRRRAAACR L* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1969>: m615.seq Length: 1116 1 ATGCGGAAAA GGCGGTGGCG CGGTTTCGGC AGCTTTGAAA AGCAGTGAGT 51 AAATGCTGCC TGCAAACCAC AATGCCGAGA GCAGGATAAA GCGGTTGCGT 101 GGCAGATTCA TGCTTGTTCC TCTTCAAGCC ATGTCTGGCA TAGTTTGGAT 151 AGGCGCAGGA ATTTTCCGCC GCGTGCGGCC AGCATATCGC GCCAAACGGC 201 AATTTCTTCG GCGGAGGGGG CATCGTCTAT GCTGCATTCG TAGAGCAGGA 251 AATCGAGGGT TTCTTCGATG ACGGGGATGG ATTCGGTTTG GATAAGCTGC 301 TTGAGTTCGG TCATGACTGT TCGGATATGG AAATCGGGAA CATGCCGTCT 351 GAAAGGGCTT CAGACGGCAT CGGGTCATTT GCTGTGCAGG AAGCGGGTTG 401 CTTCTTCCCA TTTGCCGGCA AGGATGTCGG GTATGGCTTG CAGGGATTTG 451 GCGACGGCAT CGTCAATCTG TCGGCGGTGT . TCCGTACTG GGTTTGTTCA 501 GGACATAGCC GACGACGAGG TTGCGGTCGC CCGGGTGGCC GATGCCGAGG 551 CGCAGGCGGT AATAGTCTGC CGTGCCGAGT TTTGCCTGAA TGTCTTTCAA 601 GCCGTTGTGT CCGCCGTTGC CGCCGCCGAG TTTGAATTTG ATCCGTCCGC 651 AGGGAATGTC GAGTTCGTCG TGGACGACGA GGATTTCTTC GGGTTTGATT 701 TTGTAGAACT GTGCAAGCGC GGCAACTGCC TGTCCGGAAC GGTTCATGAA 751 CGTGGCAGGT TTGAGCAGCC AAACGTCGCC GTCGGGCAGG GCGGCACGGG 801 CGACTTCGCC GAAGAATTTT TTTTCTTCTT TAAATGAAGC CTTCCATTTC 851 CACGCCAGTT CGTCGAGGAA CCAAAAACCC GCATTGTGGC GTGTCTGTTC 901 GTATTCTTTG CCCGGGTTGC CCAAGCCGAC AACCATTTTG ATTGTGTTTG 951 ACATGATATT TTCCGTGTTT CTGTCGAATG CTGTCTGAAG GCTTCAGACG 1001 GCATGGTTAT TCTTCTTGAT TTTGAACGCG TTTGCGGCGC GCTTCTTTGG 1051 GGTCGATCAA CAGCGGGCGG TACACTTCGA TGCGGTCGCC GTCGCGCAGC 1101 GGCGTGTCGT CTTTGA This corresponds to the amino acid sequence <SEQ ID 1970; ORF 615>: m615.pep Length: 372 1 MRKRRWRGFG SFEKQXVNAA CKPQCREQDK AVAWQIHACS SSSHVWHSLD 51 RRRNFPPRAA SISRQTAISS AEGASSMLHS XSRKSRVSSM TGMDSVWISC 101 LSSVMTVRIW KSGTCRLKGL QTASGHLLCR KRVASSHLPA RMSGMACRDL 151 ATASSICRRC XRTGFVQDIA DDEVAVARVA DAEAQAVIVC RAEFCLNVFQ 201 AWSAVAAAE FEFDPSAGNV EFVVDDEDFF GFDFVELCKR GNCLSGTVHE 251 RGRFEQPNVA VGQGGTGDFA EEFFFFFKXS LPFPRQFVEE PKTRIVACLF 301 VFFARVAQAD NHFDCVXHDI FRVSVECCLK ASDGMVILLD FERVCGALLW 351 GRSTAGGTLR CGRRRAAACR L* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m615/g615 86.8% identity in 371 aa overlap 10 20 30 40 50 60 m615. pep MRKRRWRGFGSFEKQXVΝAACKPQCREQDKAVAWQIHACSSSSHVWHSLDRRRΝFPPRAA I III II MMM : : II llll M M MM lllllll: II I II M II I I I g615 MWKRRRRGVGSFEEQRIDAAGKPQCGKQAEAVARQLHAASSSSHVWQILDRRRNLPPRAA 10 20 30 40 50 60 70 80 90 100 110 120 m615.pep SISRQTAISSAEGASSMLHSXSRKSRVSSMTGMDSVWISCLSSVMTVRIWKSGTCRLKGL MM: I I M : M I I I I M I I II I II I I I I II II I II I II I I I I II Ml I II I I I g615 SMSRHCATSSADGASSMLHSYSRKSRVSSMTGMDSVWISCLSSFMTVRIRKSGKCRLKGL 70 80 90 100 110 120 130 140 150 160 170 180 m615. pep QTASGHLLCRKRVASSHLPARMSGMACRDLATASSICRRCXRTGFVQDIADDEVAVARVA III : II I I II II II I II III lllllllllllllll M II I I : II II II II II g615 QTALDYLLCRKRVASSHLPEMMSGTACRDLATASSICRRCFRARFVQDVADDEVAVAGVA 130 140 150 160 170 180 190 200 210 220 230 240 m615. pep DAEAQAVIVCRAEFCLNVFQAWSAVAAAEFEFDPSAGNVEFWDDEDFFGFDFVELCKR II II II I I II I I I II I II I I I II II II I II II I II II : II II II II II II II I l| II I I g615 DAEAQAVIVCRAEFCLNVFQAWSAVAAAEFEFDPSARDVEFWDDEDFFGFDFVELCKR 190 200 210 220 230 240 250 260 270 280 290 300 m615.pep GNCLSGTVHERGRFEQPNVAVGQGGTGDFAEEFFFFFKXSLPFPRQFVEEPKTRIVACLF I I I I I I I I I I I I I I I I I : I I I I I I : I : I I I I I I I I I I M M M M M M M M M I I g615 GNRLSGTVHERGRFEQPNIAVGQGGAGNFAEEFFFFFKRSLPFPRQFVEEPKARIVAGLF 250 260 270 280 290 300 310 320 330 340 350 360 m615. pep VFFARVAQADNHFDCVXHDIFRVSVECCLKASDGMVILLDFERVCGALLWGRSTAGGTLR II II I II I II I I II I I llllllllll II II II II II II I I II I II I I I I I I II II I I I g615 VFFARVAQADNHFDCVRHDIFRVSVECGLKASDGMVILLDFERVCGALLWGRSTAGGTLR 310 320 330 340 350 360 370 m615. pep CGRRRAAACRLX I II II II I II I I g615 CGRRRAAACRLX 370 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 1971>: a615. seq 1 ATGCGGAAAC GGCGGCGGCG CGGTGTCGGC AGCTTTGAAG AGCAGCGAAT 51 AGATGCCGCC GGCAAACCAC AATGCGGAAA GCAGGCTGAA GCGGTTGCGC 101 GGCAGCTTCA TGCCGCCTCC TCGTCCAGCC ACGTTTGGCA GATTTTGGAC 151 AGGCGCAGGA ATTTGCCGCC GCGTGCGGCA AGTATGTCGC GCCATTGTGC 201 CACTTCTTCG GCGGATGGTG CGTCGTCGAT GCTGCATTCG TACAGCAGGA 251 AATCGAGGGT TTCTTCGATG ACGGGGATGG ATTCGGTTTG GATAAGCTGC 301 TTGAGTTCGG TCATGACTGT TCGGATATGG AAATCGGGAA CATGCCGTCT 351 GAAAGGGCTT CAGACGGCAT CGGGTCATTT GCTGTGCAGG AAGCGGGTTG 401 CCTCTTCACA TTTGCCGGCA AGGATGTCGG GTATGGCTTG CAGGGATTTG 451 GCGACGGCAT CGTCAATCTG TCGGCGGTG. TTCCGTACTG GGTTTGTTCA 501 GGACATAGCC GACGACGAGG TTGCGGTCGC CCGGGTGGCC GATGCCGAGG 551 CGCAGGCGGT AATAGTCTGC CGTGCCGAGT TTTGCCTGAA TGTCTTTCAA 601 GCCGTTGTGT CCACCGTTGC CGCCGCCGAG TTTGAATTTG ATCCGTCCGC 651 AGGGAATGTC GAGTTCGTCG TGGACGACGA GGATTTCTTC GGGTTTGATT 701 TTATAAAACT GCGCAAGGGC GGCAACTGCC TGTCCGGAAC GGTTCATGAA 751 CGTGGTCGGC TTGAGCAGCC AGACATCGCC GTCGGGCAGG GTAGCACGGG 801 CGACTTCGCC GAAGAATTTT TTTTCTTCTT TAAATGAAGC CTTCCATTTC 851 CACGCCAGTT CGTCGAGGAA CCAAAAACCC GCATTGTGGC GTGTCTGTTC 901 GTATTCTTTG CCCGGGTTGC CCAAGCCGAC AACCATTTTG ATTGTGTTTG 951 ACATGATATT TTCCGTGTTT CTGCCGAATG CCGTCTGAAG GCTTCAGACG 1001 GCATGGTTAT TCTTCTTGAT TTTGAACGCG TTTGCGGCGC GCTTCTTTGG 1051 GGTCGATCAA CAGCGGGCGG TACACTTCGA TGCGGTCGCC GTCGCGCAGC 1101 GGCGTGTCGT CTTTGA This corresponds to the amino acid sequence <SEQ ID 1972; ORF 615. a>: a615.pep MRKRRRRGVG SFEEQRIDAA GKPQCGKQAE AVARQLHAAS SSSHVWQILD 51 RRRΝLPPRAA SMSRHCATSS ADGASSMLHS YSRKSRVSSM TGMDSVWISC 101 LSSVMTVRIW KSGTCRLKGL QTASGHLLCR KRVASSHLPA RMSGMACRDL 151 ATASSICRRX FRTGFVQDIA DDEVAVARVA DAEAQAVIVC RAEFCLNVFQ 201 AVVSTVAAAE FEFDPSAGNV EFVVDDEDFF GFDFIKLRKG GNCLSGTVHE 251 RGRLEQPDIA VGQGSTGDFA EEFFFFFK*S LPFPRQFVEE PKTRIVACLF 301 VFFARVAQAD NHFDCV*HDI FRVSAECRLK ASDGMVILLD FERVCGALLW 351 GRSTAGGTLR CGRRRAAACR L* m615/a615 90.3% identity in 371 aa overlap 10 20 30 40 50 60 m615 .pep MRKRRWRGFGSFEKQXVNAACKPQCREQDKAVAWQIHACSSSSHVWHSLDRRRNFPPRAA II II I II II I M I : : I I llll : I MM MM lllllll: II I II I : II I I I a615 MRKRRRRGVGSFEEQRIDAAGKPQCGKQAEAVARQLHAASSSSHVWQILDRRRNLPPRAA 10 20 30 40 50 60 70 80 90 100 110 120 m615.pep SISRQTAISSAEGASSMLHSXSRKSRVSSMTGMDSVWISCLSSVMTVRIWKSGTCRLKGL I M I I II : M II I I I I I I I I I II I I I I II II II II I I I I II I I I I II I II I I I I I a615 SMSRHCATSSADGASSMLHSYSRKSRVSSMTGMDSVWISCLSSVMTVRIWKSGTCRLKGL 70 80 90 100 110 120 130 140 150 160 170 180 m615.pep QTASGHLLCRKRVASSHLPARMSGMACRDLATASSICRRCXRTGFVQDIADDEVAVARVA II II I II II II II I II II II II II II II I I I I I I II II I I I II I II II II I II II I II a615 QTASGHLLCRKRVASSHLPARMSGMACRDLATASSICRRXFRTGFVQDIADDEVAVARVA 130 140 150 160 170 180 190 200 210 220 230 240 m615. pep DAEAQAVIVCRAEFCLNVFQAWSAVAAAEFEFDPSAGNVEFWDDEDFFGFDFVELCKR I I II I I I I II I II I II I I I I II I M I I I I I I I I I II II II I I I I II I I I I I I I I :: I I a615 DAEA Q AVIVCRAEFCLNVFQAWSTVAAAEFEFDPSAGNVEFWDDEDFFGFDFIKLRKG 190 200 210 220 230 240 250 260 270 280 290 300 m615. pep GNCLSGTVHERGRFEQPNVAVGQGGTGDFAEEFFFFFKXSLPFPRQFVEEPKTRIVACLF II II II II II II I : II I : : II II I : II II I II II II I II I II II I I I I II I I I II I II I I a615 GNCLSGTVHERGRLEQPDIAVGQGSTGDFAEEFFFFFKXSLPFPRQFVEEPKTRIVACLF 250 260 270 280 290 300 310 320 330 340 350 360 m615. pep VFFARVAQADNHFDCVXHDIFRVSVECCLKASDGMVILLDFERVCGALLWGRSTAGGTLR II II II II I II II II II II II II I : II I I II I I I II I II II I II I I I I I I II I I I II I I a615 VFFARVAQADNHFDCVXHDIFRVSAECRLKASDGMVILLDFERVCGALLWGRSTAGGTLR 310 320 330 340 350 360 370 m615.pep CGRRRAAACRLX II I II II II II I a615 CGRRRAAACRLX 370 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1973>: g616.seq 1 atgtcgaaCA CAATCAAAAT GGTTGTCGGC TTGGGCAACC CGGGCAAAGA 51 ATACGAACAG ACCCGCCACA ATGCGGGCTT TTGGTTCCTC GACGAACTGG 101 CGTGGAAATG GAAGGCTTCG TTTAAAGAAG AAAAAAAATT CTTCGGCGAA 151 GTTGCCCGCG CCGCCCTGCC CGACGGCGAT GTTTGGCTGC TCAAACCGGC 201 CACGTTCATG AACCGTTCCG GACAGGCGGT TGCCGCGCTT GCACAGTTCT 251 ACAAAATCAA ACCCGAAGAA ATCCTCGTCG TCCACGACGA ACTCGACATC 301 CCTTGCGGAC GGATCAAATT CAAACTCGGC GgcggcaaCG gcgGACACAA 351 CGGCTTGAAA GACATTcagG CAAAACTCGG CACGGcagac tattaCCGCC 401 TGCGCCTCGG CATCGgccaC CCCGGCgacc gcaacctCGT CGtcggctac 451 gtcttgAACa aaccgagcgc gGaagcaccg Ccggcaaatc gacgatgCCG 501 TCGccaaATC CCTgcaggcc gtaccCGACA TcaTTTCCGg caaatgggaa 551 gaggcaacgc gcTTCCTGCA CAGCAAATAA TccaatGCCG TCTGaagccc 601 ttTcagacgg cattttcccg atttccgTAT CcGAaCagtc atgaacgaac 651 tcaagcAGcT tatCCAAAcg gaaTccatcC ccgtcatcga agaaaccctc 701 gatttcctgc tGTACGAATG cagcAtcgac gaagCAccgt ccgccgaaga 751 agtggcacaa TGgcgcgaca tactTGccgc acgcgGcgGC AAATtcCTgc 801 gcctgtccaa aatctgcCaa aCGTGGCtGG ACgAGGAGGC GGCatgAAgc 851 tGCCGcgcAA CCgcttcaGc ctgctTTCCG CATTGTGGTT TGCCGGCGGC 901 atctATtCgc tgctcttcaA AGCTGccgaC ACCGCGCCGC CGCCGTTTCC 951 ACATTtcgaC AAAGCAGCAC ACCTTGCCCT GTTTTTCGCA CAaatCTTgt 1001 tTctGGCCAA AGCATTCAAA ACCGGAAAAC TTCCCATCCC CTACCGCAGC 1051 CTGATTGCGT TCGCCTTCTG TTTTGCCGTC GGCAGCGAAT GCGCGCAGGC 1101 ATGGTTTACC GCAACGCGAA CCGGCAGTTT GGGCGATGTC CTTGCCgACC 1151 TGACGGGCGC AGCCCTTGCC CTCTTTGCCG CGCGTTCTGC CTGCCGcccg 1201 gactaa This corresponds to the amino acid sequence <SEQ ID 1974; OI g616.pep 1 MSΝTIKMVVG LGΝPGKEYEQ TRHΝAGFWFL DELAWKWKAS FKEEKKFFGE 51 VARAALPDGD VWLLKPATFM ΝRSGQAVAAL AQFYKIKPEE ILVVHDELDI 101 PCGRIKFKLG GGΝGGHΝGLK DIQAKLGTAD YYRLRLGIGH PGDRΝLVVGY 151 VLΝKPSAEAP PAΝRRCRRQI PAGRTRHHFR QMGRGΝALPA QQIIQCRLKP 201 FQTAFSRFPY PΝSHERTQAA YPΝGIHPRHR RΝPRFPAVRM QHRRSTVRRR 251 SGTMARHTCR TRRQIPAPVQ ΝLPΝVAGRGG GMKLPRΝRFS LLSALWFAGG 301 IYSLLFKAAD TAPPPFPHFD KAAHLALFFA QILFLAKAFK TGKLPIPYRS 351 LIAFAFCFAV GSECAQAWFT ATRTGΞLGDV LADLTGAALA LFAARSACRP 401 D* The following partial DΝA sequence was iidentified in N menin^ 1 ATGTCAAACA CAATCAAAAT GGTTGTCGGC TTGGGCAACC CGGGCAAAGA 51 ATACGAACAG ACACGCCACA ATGCGGGTTT TTGGTTCCTC GACGAACTGG 101 CGTGGAAATG GAAGGCTTCA TTTAAAGAAG AAAAAAAATT CTTCGGCGAA 151 GTCGCCCGTG CCGCCCTGCC CGACGGCGAC GTTTGGCTGC TCAAACCTGC 201 CACGTTCATG AACCGTTCCG GACAGGCAGT TGCCGCGCTT GCACAGTTCT 251 ACAAAATCAA ACCCGAAGAA ATCCTCGTCG TCCACGACGA ACTCGACATT 301 CCCTGCGGAC GGATCAAATT CAAACTCGGC GGCGGCAACG GCGGACACAA 351 CGGCTTGAAA GACATTCAGG CAAAACTCGG CACGGCAGAC TATTACCGCC 401 TGCGCCTCGG CATCGGCCAC CCGGGCGACC GCAACCTCGT CGTCGGCTAT 451 GTCCTGAACA AACCCAGTAC GGAACA . CCG CCGACAGATT GACGATGCCG 501 TCGCCAAATC CCTGCAAGCC ATACCCGACA TCCTTGCCGG CAAATGGGAA 551 GAAGCAACCC GCTTCCTGCA CAGCAAATGA CCCGATGCCG TCTGAAGCCC 601 TTTCAGACGG CATGTTCCCG ATTTCCATAT CCGAACAGTC ATGACCGAAC 651 TCAAGCAGCT TATCCAAACC GAATCCATCC CCGTCATCGA AGAAACCCTC 701 GATTTCCTGC TCTACGAATG CAGCATAGAC GATGCCCCCT CCGCCGAAGA 751 AATTGCCGTT TGGCGCGATA TGCTGGCCGC ACGCGGCGGA AAATTCCTGC 801 GCCTATCCAA ACTATGCCAG ACATGGCTTG AAGAGGAACA AGCATGAATC 851 TGCCACGCAA CCGCTTTATC CTGCTCTCGG CATTGTGGTT TGCAGGCAGC 901 ATTTACTCAC TGCTTTTCAA AGCTGCCGAA ACCGCGCCAC CGCCTTTTCC 951 GCATTTTGAC AAAGTGGCGC ACCTCGCCCT GTTTTTCGCA CAAATCTGGC 1001 TTCTGACCAA AGCATTCAGA ACCGACAACC GCCCCATCCC CTATCGCAGC 1051 CTGATGGTCT TTGCCCTCTG TTTCGCCCTC TTCAGCGAAT GCGCGCAGGC 1101 ATGGTTTACC GCAACGAGAA CCGGCAGTTT GGGCGATGTC CTTGCCGACC 1151 TGACGGGCGC AGCCCTTGCC CTCTTTACCG CGCGAGCTGC CTGCCGCCCG 1201 GACTAA This corresponds to the amino acid sequence <SEQ ID 1976; ORF 616>: 616.pep 1 MSNTIKMVVG LGNPGKEYEQ TRHNAGFWFL DELAWKWKAS FKEEKKFFGE 51 VARAALPDGD VWLLKPATFM NRSGQAVAAL AQFYKIKPEE ILVVHDELDI 101 PCGRIKFKLG GGNGGHNGLK DIQAKLGTAD YYRLRLGIGH PGDRNLVVGY 151 VLNKPSTEXP PTDXRCRRQI PASHTRHPCR QMGRSNPLPA QQMTRCRLKP 201 FQTACSRFPY PNSHDRTQAA YPNRIHPRHR RNPRFPALRM QHRRCPLRRR 251 NCRLARYAGR TRRKIPAPIQ TMPDMAXRGT SMNLPRNRFI LLSALWFAGS 301 IYSLLFKAAE TAPPPFPHFD KVAHLALFFA QIWLLTKAFR TDNRPIPYRS 351 L LMMVVFFAALLCCFFAALL FSECAQAWFT ATRTGSLGDV LADLTGAALA LFTARAACRP 401 DD** m616/g616 86.0% identity in 401 aa overlap 10 20 30 40 50 60 m616.pep MSNTIKMVVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEEKKFFGEVARAALPDGD I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g616 MSNTIKMVVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEEKKFFGEVARAALPDGD 10 20 30 40 50 60 70 80 90 100 110 120 m616.pep VWLLKPATFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPCGRIKFKLGGGNGGHNGLK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g616 VWLLKPATFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPCGRIKFKLGGGNGGHNGLK 70 80 90 100 110 120 130 140 150 160 170 180 m616.pep DIQAKLGTADYYRLRLGIGHPGDRNLVVGYVLNKPSTEXPPTDXRCRRQIPASHTRHPCR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M I I M: I I I I I I I I :: I I I I g616 DIQAKLGTADYYRLRLGIGHPGDRNLVVGYVLNKPSAEAPPANRRCRRQIPAGRTRHHFR 130 140 150 160 170 180 190 200 210 220 230 240 m616.pep QMGRSNPLPAQQMTRCRLKPFQTACΞRFPYPNSHDRTQAAYPNRIHPRHRRNPRFPALRM I I I Ml I I I I M : I I I I I I I I I I I I I I I I I Ml I I I I I I I I I I I I I I I I I I I I :| I g616 QMGRGNALPAQQIIQCRLKPFQTAFSRFPYPNSHERTQAAYPNGIHPRHRRNPRFPAVRM 190 200 210 220 230 240 250 260 270 280 290 300 m616.pep QHRRCPLRRRNCRLARYAGRTRRKIPAPIQTMPDMAXRGTSMNLPRNRFILLSALWFAGS llll Mil: : \ \ : : I I I I : I I I I : I : : I : : I II :| :| I I I I I g616 QHRRSTVRRRSGTMARHTCRTRRQIPAPVQNLPNVAGRGGGMKLPRNRFSLLSALWFAGG 250 260 270 280 290 300 310 320 330 340 350 360 m616.pep IYSLLFKAAETAPPPFPHFDKVAHLALFFAQIWLLTKAFRTDNRPIPYRSLMVFALCFAL I I I I I I I I I : I I I I I I I I I I Ml I I I I I I I I I : I : I I I : I : I I I I I I M : I I : I I I : g616 IYSLLFKAADTAPPPFPHFDKAAHLALFFAQILFLAKAFKTGKLPIPYRSLIAFAFCFAV 310 320 330 340 350 360 370 380 390 400 m616.pep FSECAQAWFTATRTGSLGDVLADLTGAALALFTARAACRPDX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I : I I I I I I g616 GSECAQAWFTATRTGSLGDVLADLTGAALALFAARSACRPDX 370 380 390 400 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1977>: a616. seq 1 ATGTCAAACA CAATCAAAAT GGTTGTCGGC TTGGGCAACC CGGGCAAAGA 51 ATACGAACAG ACACGCCACA ATGCGGGTTT TTGGTTCCTC GACGAACTGG 101 CGTGGAAATG GAAGGCTTCA TTTAAAGAAG AAAAAAAATT CTTCGGCGAA 151 GTCGCCCGTG CTACCCTGCC CGACGGCGAT GTCTGGCTGC TCAAGCCGAC 201 CACGTTCATG AACCGTTCCG GACAGGCAGT TGCCGCCCTT GCGCAGTTTT 251 ATAAAATCAA ACCCGAAGAA ATCCTCGTCG TCCACGACGA ACTCGACATT 301 CCCTGCGGAC GGATCAAATT CAAACTCGGC GGCGGCAACG GTGGACACAA 351 CGGCTTGAAA GACATTCAGG CAAAACTCGG CACGGCAGAC TATTACCGCC 401 TGCGCCTCGG CATCGGCCAC CCGGGCGACC GCAACCTCGT CGTCGGCTAT 451 GTCCTGAACA AACCCAGTAC GGAA.CACCG CCGACAGATT GACGATGCCG 501 TCGCCAAATC CCTGCAAGCC ATACCCGACA TCCTTGCCGG CAAATGTGAA 551 GAGGCAACCC GCTTCCTGCA CAGCAAATGA CCCGATGCCG TCTGAAGCCC 601 TTTCAGACGG CATGTTCCCG ATTTCCATAT CCGAACAGTC ATGACCGAAC 651 TCAAGCAGCT TATCCAAACC GAATCCATCC CCGTCATCGA AGAAACCCTC 701 GATTTCCTGC TGTACGAATG CAGCATCGAC GACGCACCAT CCGCCGAAGA 751 AGTGGCACAA TGGCGCGACA TACTTGCCGC ACGCGGCGGC AAATTCCTGC 801 GCCTGTCCAA AATCTGCCAA ACGTGGCTGG ACGAGGAGGC GGCATGAAGC 851 TGCCGCGCAA CCGCTTCAGC CTGCTTTCCG CATTGTGGTT TGCCGGCGGC 901 ATCTATTCGC TGCTCTTCAA AGCTGCCGAC ACCGCGCCGC CGCCGTTTCC 951 GCATTTCGAC AAAGCAGCAC ACCTTGCCCT GTTTTTCGCA CAAATCTGGC 1001 TTTTGACCAA AGCATTCAAA ACCGGAAAAC TTCCCATCCC CTACCGCAGC 1051 CTGATGGTCT TTGCCCTCTG TTTCGCCCTC TTCAGCGAAT GCGCGCAGGC 1101 ATGATTTACC GCAACGAGAA CCGGCAGTTT GGGCGATGTT CTTGCCGATA 1151 TGGCAGGTAC GGTTCTCGCA CTCTTTGCCG CCCGCGCCGC CGACCGCCCG 1201 GACTGA This corresponds to the amino acid sequence <SEQ ID 1978; ORF 616.a>: a616.pep 1 MSNTIKMVVG LGNPGKEYEQ TRHNAGFWFL DELAWKWKAS FKEEKKFFGE 51 VARATLPDGD VWLLKPTTFM NRSGQAVAAL AQFYKIKPEE ILVVHDELDI 101 PCGRIKFKLG GGNGGHNGLK DIQAKLGTAD YYRLRLGIGH PGDRNLVVGY 151 VLNKPSTEXP PTD*RCRRQI PASHTRHPCR QM*RGNPLPA QQMTRCRLKP 201 FQTACSRFPY PNSHDRTQAA YPNRIHPRHR RNPRFPAVRM QHRRRTIRRR 251 SGTMARHTCR TRRQIPAPVQ NLPNVAGRGG GMKLPRNRFS LLSALWFAGG 301 IYSLLFKAAD TAPPPFPHFD KAAHLALFFA QIWLLTKAFK TGKLPIPYRS 351 LMVFALCFAL FSECAQA*FT ATRTGSLGDV LADMAGTVLA LFAARAADRP 401 D* m616/a616 90.0% identity in 401 aa overlap 10 20 30 40 50 60 m.616 . pep MSNTIKMWGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEEKKFFGEVARAALPDGD M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M a616 MSNTIKMWGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEEKKFFGEVARATLPDGD 10 20 30 40 50 60 70 80 90 100 110 120 πι616. pep VWLLKPATFMNRSGQAVAALAQFYKIKPEEILWHDELDIPCGRIKFKLGGGNGGHNGLK II II MM I I I II I II I II II II II I II II II II I II I II I I II II I I I II I M II II II a616 VWLLKPTTFMNRSGQAVAALAQFYKIKPEEILWHDELDIPCGRIKFKLGGGNGGHNGLK 70 80 90 100 110 120 130 140 150 160 170 180 m616. pep DIQAKLGTADYYRLRLGIGHPGDRNLWGYVLNKPSTEXPPTDXRCRRQIPASHTRHPCR II II II II I II II II II II II II I II II II II II I I I I II I II II II I I I I I I II I I I I I a616 DIQAKLGTADYYRLRLGIGHPGDRNLWGYVLNKPSTEXPPTDXRCRRQIPASHTRHPCR 130 140 150 160 170 180 190 200 210 220 230 240 m616. pep QMGRSNPLPAQQMTRCRLKPFQTACΞRFPYPNSHDRTQAAYPNRIHPRHRRNPRFPALRM M M II II II II I II II II II II I II I I I II II I I I I I I I II II II II II I I II I M II a616 QMXRGNPLPAQQMTRCRLKPFQTACSRFPYPNSHDRTQAAYPNRIHPRHRRNPRFPAVRM 190 200 210 220 230 240 250 260 270 280 290 300 m616.pep QHRRCPLRRRNCRLARYAGRTRRKIPAPIQTMPDMAXRGTSMNLPRNRFILLSALWFAGS llll IM : \ \ : ι I I I I : I I I I : I : : I : : I II : M II i I I lllllllll: a616 QHRRRTIRRRSGTMARHTCRTRRQIPAPVQNLPNVAGRGGGMKLPRNRFSLLSALWFAGG 250 260 270 280 290 300 310 320 330 340 350 360 m616.pep IYSLLFKAAETAPPPFPHFDKVAHLALFFAQIWLLTKAFRTDNRPIPYRSLMVFALCFAL II I II I II I : II II II I II II : I II II II II II II I I IM I : I II I I I I II I I I II I I a616 IYSLLFKAADTAPPPFPHFDKAAHLALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFAL 310 320 330 340 350 360 370 380 390 400 m616. pep FSECAQAWFTATRTGSLGDVLADLTGAALALFTARAACRPDX lllllll I II II II II II I II I : : I : : I II I : I I I I llll a616 FSECAQAXFTATRTGSLGDVLADMAGTVLALFAARAADRPDX 370 380 390 400 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 1979>: g619.seq 1 ATGCCGTCTG AAAAAAATAT CGGTTTTATG GCAGGAAGCA GCCGTCCGTT 51 GCGGGTCGCC TTTGCGCTGT TGCTGGTTTC CTGCATCCTG TTTATGACGC 101 TCAACGTCAA AGGAGATTGG GACTTTGTCT TGCACCTGCG CCTGACCAAG 151 CTTGCCGCGC TGCTGATGGT CGCCTATGCG GTCGGCGTGT CCACTCAACT 201 CTTCCAAACG CTGACCAACA ACCCGATTCT GACCCCTTCG ATTTTGGGTT 251 TCGATTCGCT GTATGTGTTT TTGCAGACCT TGCTGgtGTT TACGTtcgGC 301 GGCGTGGGCT ATAcatccct gccgttgacg gGCAAATTCG GCTTTGAACT 351 GGTTGTTATG ATGGGCGGCT CGCTGCTGCT GTTTTACACG CTCATCCGTC 401 AGGGCGGGCG CGATTTGCCG CACATGATTT TAATCGGCGT GATTTTCGGG 451 ATTTTGTTCC GCAGCCTTTC CTCGCTGCTT TCGCGCATGA TAGACCCCGA 501 AGAATTTACC GCCGCGCAGG CGAATATGTT TGCCGGATTC AATACCGTCC 551 GCAGCGAGCT TTTAGGCATA GGCGCGCTGG TCCTGCTCGT CAGCGCGGCG 601 GTCGTTTGGC ACGAACGCTA CCGCTCGGAC GTACACCTTT TGGGGCGCGA 651 CCAAGCCGTC AATTTGGGCA TCAGCTACAC GCGCAACACC TTATGGATAC 701 TGCTTTGGAT TGCCGCATTG GTGGCGACGG CGACCGCCGT TGTCGGCCCG 751 GTGAGCTTTT TCGGGCTTCT CGCCGCCTCG CTTGCCAACC ACTTTTCCCc 801 gtCCGTGCGC CATTCCGTCC GCCTGCcgat gacggtttGC gtcgGcggCA 851 TCCTCTTGgt cggCggacaA ACCGTATTCG AACACTTCTT GGGCATGAag 901 gCggTATTAA GCGTGGTGGt cgAATTTGCG ggcggactcG TTTTCCTCTA 951 TCTCGTTTTA AAACACAAAA AATGA This corresponds to the amino acid sequence <SEQ ID 1980; ORF 619.a>: g619.pep 1 MPΞEKΝIGFM AGSSRPLRVA FALLLVΞCIL FMTLΝVKGDW DFVLHLRLTK 51 LAALLMVAYA VGVSTQLFQT LTΝΝPILTPΞ ILGFDΞLYVF LQTLLVFTFG 101 GVGYTSLPLT GKFGFELVVM MGGΞLLLFYT LIRQGGRDLP HMILIGVIFG 151 ILFRSLSΞLL SRMIDPEEFT AAQAΝMFAGF ΝTVRSELLGI GALVLLVSAA 201 VVWHERYRSD VHLLGRDQAV ΝLGIΞYTRΝT LWILLWIAAL VATATAVVGP 251 VSFFGLLAAS LAΝHFSPSVR HSVRLPMTVC VGGILLVGGQ TVFEHFLGMK 301 AVLSVVVEFA GGLVFLYLVL KHKK* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1981 >: m61 .seq 1 ATGCCGTCTG AAAAAAATAT CGGTTTTATG GCAGGAAGCA GCCGCCCGTT 51 GTGGGTCGCC TTTGCGCTGT TGCTGGTTTC CTGCGTCCTG TTTATGACGC 101 TCAACGTCAA AGGCGATTGG GATTTTGTTT TGCAACTGCG GCTGACCAAA 151 CTTGCCGCGC TGCTGATGGT CGCCTATGCG GTCGGCGTGT CCACGCAACT 201 CTTCCAAACG CTGACCAATA ATCCGATTCT GACCCCTTCA ATTTTGGGTT 251 TCGATTCGCT GTATGTGTTT TTGCAGACCT TGCTGGTGTT TACGTTCGGC 301 GGCGTGGGCT ATGCTTCCCT GCCGTTGACG GGCAAATTCG GCTTTGAACT 351 GGTCGTCATG ATGGGCGGCT CGCTGCTGCT GTTCTACACG CTCATCAAAC 401 AGGGCGGACG CGATTTGTCG CGCATGATTT TAATCGGCGT GATTTTCGGG 451 ATTTTGTTCC GCAGCCTGTC GTCGCTGCTT TCGCGCATGA TCGATCCCGA 501 AGAATTTACC GCCGCGCAGG CGAATATGTT TGCCGGATTC AATACCGTCC 551 ACAGCGAGCT TTTGGGCATA GGCGCGCTGA TTCTGCTCGT CAGCGCGGCG 601 GTCGTTTGGC GCGAACGCTA CCGCTTGGAC GTTTACCTTT TGGGGCGTGA 651 CCAAGCCGTC AATTTGGGCA TCAGCTACAC GCGCAACACC TTATGGATAC 701 TGCTTTGGAT TGCCGCATTG GTGGCGACGG CGACCGCCGT GGTCGGCCCC 751 GTAAGCTTTT TCGGGCTTCT CGCCGCCTCG CTTGCCAACC ACTTTTCCCC 801 GTCGGTCAAA CATTCCGTCC GCCTGCCGAT GACGGTTTGT ATCGGCGGCA 851 TCCTCTTGGT CGGCGGACAG ACCGTGTTCG AACACCTGCT CGGTATGCAG 901 GCAGTGTTGA GCGTAGTAGT AGAATTTGCC GGCGGACTCG TTTTCCTCTA 951 TCTCGTTTTA AAACACAAAA AATGA This corresponds to the amino acid sequence <SEQ ID 1982; ORF 619>: m619.pep 1 MPSEKNIGFM AGSΞRPLWVA FALLLVSCVL FMTLNVKGDW DFVLQLRLTK 51 LAALLMVAYA VGVSTQLFQT LTNNPILTPS ILGFDSLYVF LQTLLVFTFG 101 GVGYASLPLT GKFGFELVVM MGGSLLLFYT LIKQGGRDLS RMILIGVIFG 151 ILFRSLSΞLL SRMIDPEEFT AAQANMFAGF NTVHSELLGI GALILLVSAA 201 VVWRERYRLD VYLLGRDQAV NLGISYTRNT LWILLWIAAL VATATAVVGP 251 VΞFFGLLAAS LANHFSPSVK HSVRLPMTVC IGGILLVGGQ TVFEHLLGMQ 301 AVLΞVVVEFA GGLVFLYLVL KHKK* m619/g619 95.1% identity in 324 aa overlap 10 20 30 40 50 60 m619.pep MPSEKNIGFMAGSSRPLWVAFALLLVSCVLFMTLNVKGDWDFVLQLRLTKLAALLMVAYA l l l l l l l l l l l l l l l l l I I I I I I I I I I : I I I I I I I I I I I I I I I :| I I I I I I I I I I I I I I g619 MPSEKNIGFMAGΞΞRPLRVAFALLLVSCILFMTLNVKGDWDFVLHLRLTKLAALLMVAYA 10 20 30 40 50 60 70 80 90 100 110 120 m619.pep VGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGVGYASLPLTGKFGFELVVM I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I g619 VGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGVGYTSLPLTGKFGFELVVM 70 80 90 100 110 120 130 140 150 160 170 180 m619.pep MGGSLLLFYTLIKQGGRDLΞRMILIGVIFGILFRSLΞSLLSRMIDPEEFTAAQANMFAGF I I I I I I I I I I I I : I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g619 MGGSLLLFYTLIRQGGRDLPHMILIGVIFGILFRΞLSSLLSRMIDPEEFTAAQANMFAGF 130 140 150 160 170 180 190 200 210 220 230 240 m619.pep NTVHSELLGIGALILLVSAAVVWRERYRLDVYLLGRDQAVNLGIΞYTRNTLWILLWIAAL I I M I I I I I I I I I : I I I I I I I I I : I I I I I I: I I I I I I I I I I I I I I I I I I I I I I I I I I I I g619 NTVRSELLGIGALVLLVΞAAVVWHERYRSDVHLLGRDQAVNLGISYTRNTLWILLWIAAL 190 200 210 220 230 240 250 260 270 280 290 300 m619.pep VATATAVVGPVSFFGLLAASLANHFΞPSVKHSVRLPMTVCIGGILLVGGQTVFEHLLGMQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M I I I I I I I I Ml I I I I I I I I I I I I I : I I I : g619 VATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLPMTVCVGGILLVGGQTVFEHFLGMK 250 260 270 280 290 300 310 320 m619. pep AVLΞVVVEFAGGLVFLYLVLKHKKX I I I I I I I I I I I I I I I I I I I I I I I I I g619 AVLSVVVEFAGGLVFLYLVLKHKKX 310 320 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1983>: a619. seq 1 ATGCCGTCTG AAAAAAATAT CGGTTTTATG GCAGGAAGCA GCCGTCCGTT 51 GTGGGTTGCC TTTGCGCTGT TGCTGGTTTC CTGCATCCTG TTTATGACGC 101 TCAACGTCAA AGGCGATTGG GATTTTGTTT TGCACCTGCG CCTGACCAAG 151 CTTGCCGCGC TGCTGATGGT CGCCTATGCG GTCGGCGTTT CGACCCAGCT 201 TTTTCAAACG CTGACCAACA ATCCGATTCT GACCCCTTCG ATTTTGGGTT 251 TCGATTCGCT GTATGTGTTT TTGCAGACCT TGCTGGTGTT TACGTTCGGC 301 GGCGTGGGCT ATGCTTCCCT GCCGTTGACG GGCAAATTCG GCTTTGAACT 351 GGTCGTTATG ATGGGCGGCT CGCTGCTGCT GTTTTACACG CTCATCAAAC 401 AGGGCGGGCG CGATTTGCCG CGTATGATTT TAATCGGCGT GATTTTCGGG 451 ATTTTGTTCC GCAGCCTGTC GTCGCTGCTT TCGCGCATGA TCGACCCCGA 501 AGAATTTACG GCGGCGCAGG CGAATATGTT TGCCGGATTC AATACCGTCC 551 ACAGCGAGCT TTTAGGCATA GGCGCGCTGA TTCTGCTCGT CAGCGCGGCG 601 GTCGTTTGGC GCGAACGCTA CCGCTTGGAC GTACACCTTT TGGGGCGCGA 651 CCAAGCCATA AATTTGGGCA TCAGCTACAC GCGCAACACC TTATGGATAC 701 TGCTTTGGAT TGCCGCGCTG GTGGCGACGG CGACCGCCGT TGTCGGCCCG 751 GTAAGCTTTT TCGGGCTTCT CGCCGCCTCG CTTGCCAACC ACTTTTCCCC 801 GTCGGTCAAA CATTCCGTCC GCCTGCCGAT GACGGTTTGT GTCGGCGGCA 851 TCCTCTTGGT CGGCGGACAG ACCGTATTCG AACACTTCTT GGGCATGAAG 901 GCGGTATTAA GCGTGGTGGT CGAATTTGCG GGCGGACTCG TTTTCCTCTA 951 TCTCGTTTTA AGACACAAAA AATGA This corresponds to the amino acid sequence <SEQ ID 1984; ORF 619.a>: a619.pep 1 MPSEKNIGFM AGSSRPLWVA FALLLVSCIL FMTLNVKGDW DFVLHLRLTK 51 LAALLMVAYA VGVSTQLFQT LTNNPILTPS ILGFDSLYVF LQTLLVFTFG 101 GVGYASLPLT GKFGFELWM MGGSLLLFYT LIKQGGRDLP RMILIGVIFG 151 ILFRSLSSLL SRMIDPEEFT AAQANMFAGF NTVHSELLGI GALILLVSAA 201 WWRERYRLD VHLLGRDQAI NLGISYTRNT LWILLWIAAL VATATAWGP 251 VSFFGLLAAS LANHFSPSVK HSVRLPMTVC VGGILLVGGQ TVFEHFLGMK 301 AVLSVWEFA GGLVFLYLVL RHKK* m619/a619 97.2% identity in 324 aa overlap 10 20 30 40 50 60 m619. pep MPSEKNIGFMAGSSRPLWVAFALLLVSCVLFMTLNVKGDWDFVLQLRLTKLAALLMVAYA I II II II II I II II II II II II I II I II : I II II II I II I II I I : I I I II II II II II II a619 MPSEKNIGFMAGSSRPLWVAFALLLVSCILFMTLNVKGDWDFVLHLRLTKLAALLMVAYA 10 20 30 40 50 60 70 80 90 100 110 120 m619.pep VGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGVGYASLPLTGKFGFELWM II II I I I II II II II I I II II I I I I I II II I I II II II I II I II II I I I II II I I I II I I a619 VGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGVGYASLPLTGKFGFELWM 70 80 90 100 110 120 130 140 150 160 170 180 m619.pep MGGSLLLFYTLIKQGGRDLSRMILIGVIFGILFRSLSSLLSRMIDPEEFTAAQANMFAGF I II I I I I I I II II II II I I II I I I I I I II II II I II II I II II II I I I II II I II I II I a619 MGGSLLLFYTLIKQGGRDLPRMILIGVIFGILFRSLSSLLSRMIDPEEFTAAQANMFAGF 130 140 150 160 170 180 190 200 210 220 230 240 m619.pep NTVHSELLGIGALILLVSAAWWRERYRLDVYLLGRDQAVNLGISYTRNTLWILLWIAAL MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a619 NTVHSELLGIGALILLVSAAWWRERYRLDVHLLGRDQAINLGISYTRNTLWILLWIAAL 190 200 210 220 230 240 250 260 270 280 290 300 m619.pep VATATAWGPVSFFGLLAASLANHFSPSVKHSVRLPMTVCIGGILLVGGQTVFEHLLGMQ I II I II I I I II II II I II II II I II II I I I II II II I II I : I I II I I I I II I I II : I II: a619 VATATAWGPVΞFFGLLAASLANHFSPSVKHSVRLPMTVCVGGILLVGGQTVFEHFLGMK 250 260 270 280 290 300 310 320 mβl9. pep AVLSVWEFAGGLVFLYLVLKHKKX I II II II II II I I II I I II I : I II I a619 AVLSVWEFAGGLVFLYLVLRHKKX 310 320 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1985>: g620.seq 1 ATGAAGAAAA CCCTGTTGGc AATTGTTGCC gtTTTCGCCT TAAGTGCCTG 51 CCGGCaggcg gaAGaggcac cgccgCCTTT ACCCCGGCAG AtTAGCGacc 101 gttcggtcgg aCACTAttgC Agtatgaacc tgaccgaaca caacggcccc 151 aaagcccaga tttttttgaa cGGCAAACCC GATCAGCCCG TTTGGTTCTC 201 CACCGTcaag cagatgttcg GCTATACCAA GCTGCCCGAA GAGCCCAAAG 251 GCATCCGCGT GATTTACGTT ACCGATATGG GCAATGTTAC CGATTGGACG 301 AATCCTAATG CCGACACGGA GTGGATAGAT GCGAAAAAAG CCTTTTACGT 351 CATCGACAGC GGCTTTATCG GCGGTATGGG CGCGGAAGAC GCGCTGCCGT 401 TCGGCAACAA GGAGCAGGCT GAAAAATTTG CAAAGGATAA AGGCGGCAAG 451 GTCGTCGGTT TTGACGATAT GCCCGATGCT TACATTTTCA AGTAA This corresponds to the amino acid sequence <SEQ ID 1986; ORF 620.ng>: g620.pep 1 MKKTLLAIVA VFALΞACRQA EEAPPPLPRQ ISDRSVGHYC ΞMNLTEHNGP 51 KAQIFLNGKP DQPVWFΞTVK QMFGYTKLPE EPKGIRVIYV TDMGNVTDWT 101 NPNADTEWID AKKAFYVIDS GFIGGMGAED ALPFGNKEQA EKFAKDKGGK 151 VVGFDDMPDA YIFK* The following partial DNA sequence was identified in N meningitidis <SEQ ID 1987>: m620.seq 1 ATGAAAAAAA CCCTGTTGGC AATTGTTGCC GTTTCCGCCT TAAGTGCCTG 51 CCGGCAGGCG GAAGAGGGAC CGCCGCCTTT ACCCCGGCAG ATTAGCGACC 101 GTTCGGTCGG ACACTATTGC AGTATGAACC TGACCGAACA CAACGGCCCC 151 AAAGCCCAGA TTTTCTTGAA CGGCAAACCC GATCAGCCCG TTTGGTTCTC 201 CACCATCAAG CAGATGTTCG GCTATACCAA GCTGCCCGAA GAGCCTAAAG 251 GCATCCGCGT GATTTACGTT ACCGATATGG GCAATGTTAC CGATTGGACG 301 AATCCCAATG CCGACACGGA GTGGATGGAT GCGAAAAAAG CCTTTTACGT 351 CATCGACAGC GGCTTTATCG GCGGTATGGG TGCGGAAGAC GCGCTGCCGT 401 TCGGCAACAA AGAGCAGGCT GAGAAATTTG CAAAGGATAA AGGCGGTAAG 451 GTTGTCGGTT TCGACGATAT GCCTGATACC TATATTTTCA AATAA This corresponds to the amino acid sequence <SEQ ID 1988; ORF 620>: m620.pep 1 MKKTLLAIVA VSALSACRQA EEGPPPLPRQ ISDRSVGHYC SMΝLTEHΝGP 51 KAQIFLNGKP DQPVWFSTIK QMFGYTKLPE EPKGIRVIYV TDMGNVTDWT 101 NPNADTEWMD AKKAFYVIDS GFIGGMGAED ALPFGNKEQA EKFAKDKGGK 151 VVGFDDMPDT YIFK* m620/g620 97.0% identity in 164 aa overlap 10 20 30 40 50 60 m620.pep MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNLTEHNGPKAQIFLNGKP I I I I I I I I I I I I I I I II I II l I II I II I I I I II I I I I I I I II I I I I I I I I I I I I I I I g620 MKKTLLAIVAVFALΞACRQAEEAPPPLPRQISDRSVGHYCΞMNLTEHNGPKAQIFLNGKP 10 20 30 40 50 60 70 80 90 100 110 120 m620 . pep DQPVWFΞTIKQMFGYTKLPEEPKGIRVI VTDMGNVTDWTNPNADTEWMDAKKAFYVIDS I I I I I I I I M I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I l I I I I I I I I I I g620 DQPVWFΞTVKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNPNADTEWIDAKKAFYVIDS 70 80 90 100 110 120 130 140 150 160 m620. pep GFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDTYIFKX I I I I I I I I I I I I I I I I II I II I I I I II I II I I I I I I I I l I I I I g620 GFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDAYIFKX 130 140 150 160 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1989>: a620. seq 1 ATGAAAAAAA CCCTGTTGGC AATTGTTGCC GTTTCCGCCT TAAGTGCCTG 51 CCGGCAGGCG GAAGAGGGAC CGCCGCCTTT ACCCCGGCAG ATTAGCGACC 101 GTTCGGTCGG ACACTATTGC AGTATGAACC TGACCGAACA CAACGGCCCC 151 AAAGCCCAGA TTTTCTTGAA CGGCAAACCC GATCAGCCCG TTTGGTTCTC 201 CACCATCAAG CAGATGTTCG GCTATACCAA GCTGCCCGAA GAGCCTAAAG 251 GCATCCGCGT GATTTACGTT ACCGATATGG GCAATGTTAC CGATTGGACG 301 AATCCCAATG CCGACACGGA GTGGATGGAT GCGAAAAAAG CCTTTTACGT 351 CATCGACAGC GGCTTTATCG GCGGTATGGG TGCGGAAGAC GCGCTGCCGT 401 TCGGCAACAA AGAGCAGGCT GAGAAATTTG CAAAGGATAA AGGCGGTAAG 451 GTTGTCGGTT TCGACGATAT GCCTGATACC TATATTTTCA AATAA This corresponds to the amino acid sequence <SEQ ID 1990; ORF 620.a>: a620.pep 1 MKKTLLAIVA VSALSACRQA EEGPPPLPRQ ISDRSVGHYC SMΝLTEHΝGP 51 KAQIFLNGKP DQPVWFSTIK QMFGYTKLPE EPKGIRVIYV TDMGNVTDWT 101 NPNADTEWMD AKKAFYVIDS GFIGGMGAED ALPFGNKEQA EKFAKDKGGK 151 VVGFDDMPDT YIFK* m620/a620 100.0% identity in 164 aa overlap 10 20 30 40 50 60 m620.pep MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNLTEHNGPKAQIFLNGKP II II II II II II I II II II II II II II I II I I II I I II I II I I II II I I I I I I II I I I II a620 MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNLTEHNGPKAQIFLNGKP 10 20 30 40 50 60 70 80 90 100 110 120 m620.pep DQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNPNADTEWMDAKKAFYVIDS II II II II II II II II I II II II II II II II II I II II II II I II II II II I I I I I II I I a620 DQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNPNADTEWMDAKKAFYVIDS 70 80 90 100 110 120 130 140 150 160 m620. pep GFIGGMGAEDALPFGNKEQAEKFAKDKGGKWGFDDMPDTYIFKX II II II II II II I II II I II II I II II I II I II I II II II II II I a620 GFIGGMGAEDALPFGNKEQAEKFAKDKGGKWGFDDMPDTYIFKX 130 140 150 160 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1991>: g622.seq 1 ATGCAactta ccgctgtcgg ACTCAATCAT CAAACCGCAC CTTTAAGCAT 51 ACGGGAAAag ctggCGTTTG CCGCCGCCGC CCTGCCAGAA gccgTccgCA 101 ATCTTGCCCG AAGCAATGCG GCAACGGAGG CGGTAATCCT TTCTACCTGC 151 AACCGCACCG AGCTTTACTG CGTCGGCGAT TCGGAAgaaa TCATCCGATG 201 GCTTGCCGAT TACCACAGTT TGCCGATTGA AGAAATCCGT CCGTATCTGT 251 ACACGCTGGA TATGCAGGAA ACCGTGCGCC ACGCCTTCCG CGTTGCCTGC 301 GGCTTGGATT CGATGGTTTT GGGCGAGCCG CAGATTTTGG GGCAGATTAA 351 AGATGCGGTG CGTGCGGCTC AAGAACAGGA AAGTATGGGG GCAAAACTCA 401 ATGCCCTGTT CCAAAAAACC TTTTCCGTTG CTAAAGAAGT CCGTACCGAT 451 ACCGCTGTCG GCGAAAATTC GGTTTCGATG GCTTCCGCGT CCGTCAAGTT 501 GGCGGAACAG ATTTTTCCCG ACATCGGCGA TTTGAACGTA TTGTTTATCG 551 GCGCAGGCGA AATGATTGAG CTGGTTGCCA CTTATTTTGC CGCCAAAAAT 601 CCCCGGCTGA TGACGGTTGC CAACCGGACG CTGGCGCGTG CACAGGAGTT 651 GTGCGACAAG CTCGGTGTTA ACGCCGAACC GTGCCTGCTG TCCGATCTGC 701 CTGCCATTCT GCACGATTAC GACGTGGTGG TTTCTTCAAC GGCGAGCCAG 751 CTTCCGATAG TCGGCAAAGG CATGGTCGAA CGCGCATTGA AACAGCGTCA 801 GAGTATGCCG TTGTTCATGC TTGACTTGGC CGTGCCGCGC GATATTGAAG 851 CGGAAGTCGG CGATTTGAAC GATGCGTATC TTTATACGGT GGACGATATG 901 GTCAACATCG TCCAAAGCGg caaggaggca aggcagaaag ccgccgcCgc 951 cgccgaaacg ctggTGTCCG AAAAGGTTGC CGAATTTGTC AGGCAGCAGC 1001 AGGGCAGGCA GagcgttcCG CTGATTAAGG CCTTGCGGGA CGAGGGCGAG 1051 AAAGCGCGCA AGCAGGTGTT GGAAAATGCG ATGAAACAGC TTGCCAAAGG 1101 CGcaaCGGCG GAAGaggttt TGgaacggct gtccgtcCAA CTGACCAACA 1151 AGCTGCTGCA TTCGCCAACT CAAACCTTGA ATAAGGCGGG GGAAGAAGAT 1201 AAAGatttGG TTCATGCCgt cGCGCAGATt tatcatttGG ACAAATAA This corresponds to the amino acid sequence <SEQ ID 1992; ORF 622.ng>: g622.pep 1 MQLTAVGLΝH QTAPLSIREK LAFAAAALPE AVRΝLARSΝA ATEAVILSTC 51 ΝRTELYCVGD SEEIIRWLAD YHΞLPIEEIR PYLYTLDMQE TVRHAFRVAC 101 GLDSMVLGEP QILGQIKDAV RAAQEQESMG AKLΝALFQKT FSVAKEVRTD 151 TAVGEΝSVSM ASASVKLAEQ IFPDIGDLΝV LFIGAGEMIE LVATYFAAKΝ 201 PRLMTVAΝRT LARAQELCDK LGVΝAEPCLL SDLPAILHDY DVVVΞSTASQ 251 LPIVGKGMVE RALKQRQSMP LFMLDLAVPR DIEAEVGDLΝ DAYLYTVDDM 301 VΝIVQSGKEA RQKAAAAAET LVSEKVAEFV RQQQGRQΞVP LIKALRDEGE 351 KARKQVLEΝA MKQLAKGATA EEVLERLΞVQ LTΝKLLHSPT QTLΝKAGEED 401 KDLVHAVAQI YHLDK* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1993>: m622.seq 1 ATGCAACTTA CCGCTGTCGG ACTCAATCAT CAAACCGCAC CTTTAAGCAT 51 ACGGGAAAAG CTGGCGTTTG CCGCCGCCGC CCTGCCTAAA GCCGTCCGCA 101 ATCTTGCCCG AAGCAATGCG GCAACGGAGG CGGTAATCCT TTCTACCTGC 151 AACCGCACCG AGCTTTACTG CGTCGGTGAT TCGGAAGAAA TCATCCGATG 201 GCTTGCCGAT TACCACAGTT TGCCGATTGA AGAAATCCGT CCGTATCTGT 251 ACGCGCTGGA TATGCAGGAG ACTGTGCGCC ATGCTTTCCG CGTCGCCTGC 301 GGGCTGGATT CGATGGTGTT GGGCGAGCCG CAGATTTTAG GACAGATTAA 351 GGATGCCGTT AGGGTTGCTC AAGAGCAGGA AAGTATGGGT AAGAAACTCA 401 ATGCCCTGTT CCAAAAAACC TTTTCCGTTG CTAAAGAGGT CCGTACCGAT 451 ACTGCCGTCG GCGAAAACTC GGTTTCCATG GCTTCCGCTT CCGTCAAATT 501 GGCGGAACAG ATTTTTCCCG ACATCGGCGA TTTGAATGTC TTGTTTATCG 551 GCGCAGGCGA AATGATTGAG CTGGTTGCCA CTTATTTTGC CGCCAAAAGT 601 CCCCGGCTGA TGACGGTTGC CAACCGGACG CTGGCGCGTG CACAGGAGTT 651 GTGCGACAAG CTCGGTGTCA ACGCCGAACC GTGCCTGCTG TCCGATCTGC 701 CTGCCATTCT GCACGATTAC GACGTAGTGG TTTCTTCAAC GGCAAGCCAG 751 TTGCCCATTG TCGGCAAAGG CATGGTGGAG CGTGCATTGA AACAAAGGCA 801 GAGTATGCCG TTGTTCATGC TTGATTTGGC AGTGCCGCGT GACATTGAAG 851 CGGAAGTCGG CGATTTGAAT GATGCCTATC TTTATACGGT GGACGATATG 901 GTCAATATCG TCCAAAGCGG CAAGGAGGCA AGGCAGAAGG CCGCCGCCGC 951 CGCCGAAACG CTGGTGTCCG AGAAAGTTGC CGAATTTGTC AGGCAGCAGC 1001 AGGGCAGGCA GAGTGTCCCC TTGATTAAGG CGTTGCGGGA CGAGGGCGAG 1051 AAAGCGCGCA AACAGGTGTT GGAAAATGCC ATGAAACAGC TTGCCAAAGG 1101 CGCAACGGCA GAAGAGGTTT TGGAACGGCT GTCCGTCCAA CTGACCAACA 1151 AGCTGCTGCA TTCGCCGACC CAAACCTTGA ATAAGGCGGG GGAAGAAGAT 1201 AAAGATTTGG TTCATGCCGT CGCGCAGATT TATCATTTGG ACAAATAA This corresponds to the amino acid sequence <SEQ ID 1994; OI m622.pep 1 MQLTAVGLNH QTAPLSIREK LAFAAAALPK AVRNLARSNA ATEAVILSTC 51 NRTELYCVGD SEEIIRWLAD YHSLPIEEIR PYLYALDMQE TVRHAFRVAC 101 GLDSMVLGEP QILGQIKDAV RVAQEQESMG KKLNALFQKT FSVAKEVRTD 151 TAVGENSVSM ASASVKLAEQ IFPDIGDLNV LFIGAGEMIE LVATYFAAKS 201 PRLMTVANRT LARAQELCDK LGVNAEPCLL SDLPAILHDY DVVVSSTASQ 251 LPIVGKGMVE RALKQRQSMP LFMLDLAVPR DIEAEVGDLN DAYLYTVDDM 301 VNIVQSGKEA RQKAAAAAET LVSEKVAEFV RQQQGRQSVP LIKALRDEGE 351 KARKQVLENA MKQLAKGATA EEVLERLSVQ LTNKLLHSPT QTLNKAGEED 401 KDLVHAVAQI YHLDK* 622/g622 98.8% identity in 415 aa overlap 10 20 30 40 50 60 m622.pep MQLTAVGLNHQTAPLSIREKLAFAAAALPKAVRNLARSNAATEAVILSTCNRTELYCVGD I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g622 MQLTAVGLNHQTAPLΞIREKLAFAAAALPEAVRNLARSNAATEAVILSTCNRTELYCVGD 10 20 30 40 50 60 70 80 90 100 110 120 m622.pep SEEIIRWLADYHΞLPIEEIRPYLYALDMQETVRHAFRVACGLDSMVLGEPQILGQIKDAV I I I I I I I I I I I I I I I I I I I I I I I M I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g622 ΞEEIIRWLADYHΞLPIEEIRPYLYTLDMQETVRHAFRVACGLDSMVLGEPQILGQIKDAV 70 80 90 100 110 120 130 140 150 160 170 180 m622. pep RVAQEQEΞMGKKLNALFQKTFSVAKEVRTDTAVGENSVΞMAΞASVKLAEQIFPDIGDLNV M I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g622 RAAQEQESMGAKLNALFQKTFSVAKEVRTDTAVGENSVΞMASAΞVKLAEQIFPDIGDLNV 130 140 150 160 170 180 190 200 210 220 230 240 m622.pep LFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELCDKLGVNAEPCLLSDLPAILHDY I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g622 LFIGAGEMIELVATYFAAKNPRLMTVANRTLARAQELCDKLGVNAEPCLLSDLPAILHDY 190 200 210 220 230 240 250 260 270 280 290 300 m622.pep DVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDLAVPRDIEAEVGDLNDAYLYTVDDM I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g622 DVVVSSTAΞQLPIVGKGMVERALKQRQSMPLFMLDLAVPRDIEAEVGDLNDAYLYTVDDM 250 260 270 280 290 300 310 320 330 340 350 360 m622. pep VNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQGRQΞVPLIKALRDEGEKARKQVLENA I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I g622 VNIV Q SGKEARQKAAAAAETLVSEKVAEFVRQQQGRQSVPLIKALRDEGEKARKQVLENA 310 320 330 340 350 360 370 380 390 400 410 m622. pep MKQLAKGATAEEVLERLSVQLTNKLLHΞPTQTLNKAGEEDKDLVHAVAQI HLDKX I I I I I II I I I I I I I I I I I I I I I I I I I I ll I I I I I I I I I I I l I I I || I I I I i i i i i i g622 MKQLAKGATAEEVLERLSVQLTNKLLHSPTQTLNKAGEEDKDLVHAVAQIYHLDKX 370 380 390 400 410 The following partial DNA sequence was identified in N meningitidis <SEQ ID 1995>: a622. seq 1 ATGCAACTTA CCGCTGTCGG ACTCAATCAT CAAACCGCAC CTTTAAGCAT 51 ACGGGAAAAG CTGGCGTTTG CCGCGGCCTG CCTGCCCGAA GCCGTCCGCA 101 ATCTTGCCCG AAGCAATGCG GCAACGGAGG CGGTAATCCT TTCTACCTGC 151 AACCGTACCG AGCTTTACTG TGTAGGTGAT TCGGAAGAAA TCATCCGTTG 201 GCTCGCAGAC TATCACAGCC TTCCCATAGA AGAAATCAGC CCCTACCTTT 251 ATACTTTGGG GATGCAGGAG ACTGTGCGCC ATGCTTTCCG CGTCGCCTGC 301 GGCTTGGATT CGATGGTGTT GGGCGAGCCG CAGATTTTAG GACAGATTAA 351 GGATGCGGTC AGGGTTGCTC AAGAGCAGGA AAGTATGGGT AAGAAACTCA 401 ATGCCCTGTT CCAAAAAACC TTTTCTGTTG CTAAAGAGGT CCGTACCGAT 451 ACTGCCGTCG GCGAAAACTC GGTTTCCATG GCTTCCGCTT CCGTCAAGTT 501 GGCAGAGCAG ATTTTCCCCG ACATCGGCGA TTTGAATGTC TTGTTTATCG 551 GTGCGGGTGA GATGATTGAG CTGGTTGCCA CTTATTTTGC CGCCAAAAGT 601 CCCCGGCTGA TGACGGTTGC CAACCGGACG CTGGCGCGTG CACAGGAGTT 651 GTGCGACAAG CTCGGTGTCA ACGCCGAACC GTGCCTGCTG TCCGATCTGC 701 CTGCCATTTT GCATGAGTAC GACGTGGTGG TTTCTTCAAC GGCAAGCCAG 751 TTGCCCATTG TCGGCAAAGG TATGGTGGAG CGCGCATTGA AACAAAGGCA 801 GAGTATGCCG TTGTTTATGC TTGACTTGGC CGTGCCGCGA GACATTGAGG 851 CGGAAGTCGG AGATTTGAAC GATGCCTATC TTTATACGGT GGACGATATG 901 GTCAATATCG TCCAAAGCGG CAAGGAGGCA AGGCAGAAGG CCGCCGCCGC 951 CGCCGAAACG CTGGTGTCCG AGAAGGTTGC CGAATTTGTC AGGCAGCAGC 1001 AGGGCAGGCA GAGTGTCCCG TTAATCAGGG CATTGAGGGA TGAGGGAGAG 1051 AAAGCGCGCA AACAGGTCTT GGAAAATGCG ATGAAACAGC TTGCCAAAGG 1101 CGCAACGGCA GAAGAGGTTT TGGAAAGGCT GTCGATCCAA CTGACCAACA 1151 AGCTGCTGCA TTCGCCGACC CAAACCTTGA ATAAGGCGGG GGAAGAAGAT 1201 AAAGATTTGG TTCACGCCGT CGCGCAGATT TATCATTTGG ACAAATAA This corresponds to the amino acid sequence <SEQ ID 1996; ORF 622. a>: a622.pep 1 MQLTAVGLΝH QTAPLSIREK LAFAAACLPE AVRΝLARSΝA ATEAVILSTC 51 ΝRTELYCVGD SEEIIRWLAD YHSLPIEEIS PYLYTLGMQE TVRHAFRVAC 101 GLDSMVLGEP QILGQIKDAV RVAQEQESMG KKLNALFQKT FSVAKEVRTD 151 TAVGENSVSM ASASVKLAEQ IFPDIGDLNV LFIGAGEMIE LVATYFAAKS 201 PRLMTVANRT LARAQELCDK LGVNAEPCLL SDLPAILHEY DVVVSSTASQ 251 LPIVGKGMVE RALKQRQSMP LFMLDLAVPR DIEAEVGDLN DAYLYTVDDM 301 VNIVQSGKEA RQKAAAAAET LVSEKVAEFV RQQQGRQSVP LIRALRDEGE 351 KARKQVLENA MKQLAKGATA EEVLERLSIQ LTNKLLHSPT QTLNKAGEED 401 KDLVHAVAQI YHLDK* m622/a622 98.1% identity in 415 aa overlap 10 20 30 40 50 60 m622. pep MQLTAVGLNHQTAPLSIREKLAFAAAALPKAVRNLARSNAATEAVILΞTCNRTELYCVGD II II II II II II II II I I II I II II I II : II II I I II II II I I I I II II I I II II I I I I a622 MQLTAVGLNHQTAPLSIREKLAFAAACLPEAVRNLARSNAATEAVILSTCNRTELYCVGD 10 20 30 40 50 60 70 80 90 100 110 120 m622.pep SEEIIRWLADYHSLPIEEIRPYLYALDMQETVRHAFRVACGLDSMVLGEPQILGQIKDAV II I II II II II II II I I I I MMM II II II II I II II II I I I I I II I I I II II I I I I a622 SEEIIRWLADYHSLPIEEISPYLYTLGMQETVRHAFRVACGLDSMVLGEPQILGQIKDAV 70 80 90 100 110 120 130 140 150 160 170 180 m622. pep RVAQEQESMGKKLNALFQKTFΞVAKEVRTDTAVGENSVSMASASVKLAEQIFPDIGDLNV I M II II II I I II II II I I II I II II I II II I II I II I M I M I I I I I I I M M I I M M a622 • RVA Q E Q ESMGKKLNALFQKTFSVAKEVRTDTAVGENSVSMASASVKLAEQIFPDIGDLNV 130 140 150 160 170 180 190 200 210 220 230 240 m622. pep LFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELCDKLGVNAEPCLLSDLPAILHDY I I II II I II I II II I II II II I II II I II I II II II I I II I II II II II I I II II II I : I a622 LFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELCDKLGVNAEPCLLSDLPAILHEY 190 200 210 220 230 240 250 260 270 280 290 300 m622. pep DVWSSTASQLPIVGKGMVERALKQRQSMPLFMLDLAVPRDIEAEVGDLNDAYLYTVDDM II I II I I II I I I II I II II II II II II II I II II II I I II I II II II I I I I I II II I II I a622 DWVSSTASQLPIVGKGMVERALKQRQSMPLFMLDLAVPRDIEAEVGDLNDAYLYTVDDM 250 260 270 280 290 300 310 320 330 340 350 360 m622. pep VNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQGRQSVPLIKALRDEGEKARKQVLENA II I II I II II II II II II II I II II II I II II II I II II II M II II I I II I II II II II a622 VNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQGRQSVPLIRALRDEGEKARKQVLENA 310 320 330 340 350 360 370 380 390 400 410 m622. pep MKQLAKGATAEEVLERLSVQLTNKLLHSPTQTLNKAGEEDKDLVHAVAQIYHLDKX II II II I I II II I II II I : I II II I I II II I II II II II I II I II II I II II II II a622 MKQLAKGATAEEVLERLSIQLTNKLLHSPTQTLNKAGEEDKDLVHAVAQIYHLDKX 370 380 390 400 410 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 1997>: g624.seq 1 ATGATCCGTT ATCTTTTAAT TGCCTGCGGC GGCATCTCCC TGCTGTTGGG 51 GATAATCGGC ATTTTTTTGC CGCTGTTGCC GACCACGCCG TTCGTACTAC 101 TCTCCGCCGC CTGCTGGGCA AAGGCAtccc cgcgcTTTCa CCgCTGGCTG 151 CACcgGCacc gCTATTTCGG CCCGATGGTT CATAACTGGG AACAAAACGG 201 CGCAGTGCCG CGCAAAGCCA AGATTTTCGC CATCAGCATG AtaaccgcAt 251 cctgcctcat gatctTTtgg CattTTCccc aacnctggtg ggtcGGGGCG 301 GTTTCATCGG TTTTTTGTTC CCTTGTcacC ATacggatgt gGcacAGacC 351 cgaatCTTGA This corresponds to the amino acid sequence <SEQ ID 1998; ORF 624.ng>: g624.pep 1 MIRYLLIACG GIΞLLLGIIG IFLPLLPTTP FVLLSAACWA KASPRFHRWL 51 HRHRYFGPMV HΝWEQΝGAVP RKAKIFAISM ITAΞCLMIFW HFPQXWWVGA 101 VSSVFCSLVT IRMWHRPES* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 1999>: m624. seq 1 ATGATACGTT ATCTTTTAAT TGCCTGCGGC TGCATTTCCC TACTGTTGGG 51 TATCATCGGC ATTTTTTTGC CGCTGTTGCC GACCACGCCG TTCGTACTGC 101 TCTCCGCCGC CTGCTGGGCA AAGGCATCCC CGCGCTTTTA CCGCTGGCTG 151 CACCGGCACC GCTATTTCGG CCCGATGGTT CATAACTGGG AACAAAACGG 201 CGCAGTGCCG CGCAAAGCCA AAATATTCGC CATCAGTATG ATGACCGCAT 251 CCTGCCTGAT AATGTTTTGG CAGTTTCCCC AACGCTGGTG GGTCGGGGCG 301 GTTTCATCGG TTTTTTGTTC CCTTGTCGCC ATATGGATGT GGCGCAGGCC 351 CGAATCTTGA This corresponds to the amino acid sequence <SEQ ID 2000; ORF 624>: 624.pep 1 MIRYLLIACG CIΞLLLGIIG IFLPLLPTTP FVLLSAACWA KASPRFYRWL 51 HRHRYFGPMV HΝWEQΝGAVP RKAKIFAISM MTASCLIMFW QFPQRWWVGA 101 VSSVFCSLVA IWMWRRPES* m624/g624 91.6% identity m 119 aa overlap 10 20 30 40 50 60 m624.pep MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKAΞPRFYRWLHRHRYFGPMV I I I M I I II I II I I I I II I I I I I I I I I I II I I I I l I I II I I II II I I g624 MIRYLLIACGGISLLLGIIGIFLPLLPTTPFVLLSAACWAKASPRFHRWLHRHRYFGPMV 10 20 30 40 50 60 70 80 90 100 110 120 m624.pep HNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVSSVFCSLVAIWMWRRPESX I ! I I I I I I I I I I I I I I I I I I : I I I I I :: I I : I I I I I I I I I I I I I I I I I : I I I : I I I I I g624 HNWEQNGAVPRKAKIFAISMITASCLMIFWHFPQXWWVGAVSSVFCSLVTIRMWHRPESX 70 80 90 100 110 120 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2001>: a624. seq 1 ATGATACGTT ATCTTTTAAT TGCCTGCGGC TGCATTTCCC TGCTGTTGGG 51 TATCATCGGC ATTTTTTTGC CGCTGTTGCC GACCACGCCG TTCGTACTGC 101 TCTCCGCCGC CTGCTGGGCA AAGGCATCCC CGCGCTTTCA CCGCTGGCTG 151 CACCGGCACC GCTATTTCGG TCCGATGGTT CATAACTGGG AACAAAACGG 201 CGCAGTGCCG CGCAAAGCCA AAATATTCGC CATCAGTATG ATGACCGCAT 251 CCTGCCTGAT AATGTTTTGG CAGTTTCCCC AACGCTGGTG GGTCGGGGCG 301 GTTTCATCGG TTTTTTGTTC CCTTGTCGCC ATATGGATGT GGCGCAGGCC 351 CGAATCTTGA This corresponds to the amino acid sequence <SEQ ID 2002; ORF 624. a>: a624.pep 1 MIRYLLIACG CISLLLGIIG IFLPLLPTTP FVLLSAACWA KASPRFHRWL 51 HRHRYFGPMV HΝWEQΝGAVP RKAKIFAISM MTASCLIMFW QFPQRWWVGA 101 VSSVFCSLVA IWMWRRPES* m624/a624 99.2% identity in 119 aa overlap 10 20 30 40 50 60 m624.pep MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASPRFYRWLHRHRYFGPMV II II I II I I I II I II I I I I II I I I II I II II II I II II II II II I I : I I II II II II I II a624 MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASPRFHRWLHRHRYFGPMV 10 20 30 40 50 60 70 80 90 100 110 120 m624 . pep HΝWEQΝGAVPRKAKI FAISMMTASCLIMFWQFPQRWWVGAVSSVFCSLVAIWMWRRPESX M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M a624 HΝWEQΝGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVSSVFCSLVAIWMWRRPESX 70 80 90 100 110 120 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2003>: a625. seq i ATGTTTGCAA CCAGGAAAAT GAAGAAGATG ACGATGTGCA CGCGGCGGGT 51 ACGGTTTTGG TTGGCTTTCA GCAGCGGACG AATCATCAGC ATTGCTGCGC 101 CGGTCGTTCC CATGATAGAG GCAAGTGCCG TACCGACGGC AAGCAGGGCG 151 GTGTTGAGCT TGGGTGTGCC GTTCAAGTCG CCCCAAACCA AAATGCCGCC 201 TGAAATGGTG TACAGGGCAA GCAGCAGCAG GATGAAAGGG ATGTATTCTT 251 CAACGAGTGC GTGTGCGACG GTATGGATAC CGGCGGACGC GCCAAAAACC 301 AAACTGAACG GGATGAGGAA GAGCAATGTC CAAAAGGCGG TAATTTTGCC 351 GTAA The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 2004>: g625.seq 1 atGTTTGCAA CCAGGAAAAT GAAGAAGATG ACGATGTGCA CGCGGCGGGT 51 ACGGtcTTGG TTGGCTTTCA GCAGCGGACG AATCATCAGC AttgCCGCGC 101 CGGtcgttcC CATGATAGAG GCAAGTGCCG TACCGACGGC AAGCAGGGCG 151 GTGTTGAGCT TGGGTGTGCC GTTCAAGTCG CCCCAAACCA AAATGCCGCC 201 TGAAATGGTG TACAGGGCAA GCAGCAGCAG GATGAAGGGG ATATATTCTT 251 CAACGAGTGC GTGTGCGACG GTATGGATAC CGGCGGACGC GCCAAAAACC 301 AAACTGAACG GGATGAGGAA GAGCAATGTC CAAAAGGCGG TGATTTTGCc 351 gtAA This corresponds to the amino acid sequence <SEQ ID 2005; ORF 625. ng>: g625.pep 1 MFATRKMKKM TMCTRRVRSW LAFSSGRIIS IAAPVVPMIE ASAVPTASRA 51 VLSLGVPFKS PQTKMPPEMV YRASSSRMKG IYSSTSACAT VWIPADAPKT 101 KLNGMRKSNV QKAVILP* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2006>: m625.seq 1 ATGTTTGCAA CCAGGAAAAT GAAGAAGATG ACGATGTGCA CGCGGCGGGT 51 ACGGTTTTGG TTGGCTTTCA GCAGCGGACG AATCATCAGC ATTGCTGCGC 101 CGGTCGTTCC CATGATAGAG GCAAGTGCCG TACCGACGGC AAGCAGGGCG 151 GTGTTGAGCT TGGGTGTGCC GTTCAAGTCG CCCCAAACCA AAATGCCGCC 201 TGAAATGGTG TACAGGGCAA GCAGCAGCAG GATGAAAGGG ATGTATTCTT 251 CAACGAGTGC GTGTGCGACG GTATGGATAC CGGCGGACGC GCCAAAAACC 301 AAACTGAACG GGATGAGGAA GAGCAATGTC CAAAAGGCGG TAATTTTGCC 351 GTAA This corresponds to the amino acid sequence <SEQ ID 2007; ORF 625>: m625.pep 1 MFATRKMKKM TMCTRRVRFW LAFSSGRIIS IAAPVVPMIE ASAVPTASRA 51 VLSLGVPFKS PQTKMPPEMV YRASSSRMKG MYSSTSACAT VWIPADAPKT 101 KLΝGMRKSΝV QKAVILP* m625/g625 98.3% identity in 117 aa overlap 10 20 30 40 50 60 m625.pep MFATRKMKKMTMCTRRVRFWLAFSSGRIISIAAPVVPMIEASAVPTASRAVLSLGVPFKS I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I g625 MFATRKMKKMTMCTRRVRSWLAFSSGRIISIAAPVVPMIEASAVPTASRAVLSLGVPFKS 10 20 30 40 50 60 70 80 90 100 110 m625.pep PQTKMPPEMVYRASSSRMKGMYSSTSACATVWIPADAPKTKLΝGMRKSΝVQKAVILPX I I I I II I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g625 PQTKMPPEMVYRAΞSSRMKGIYSSTSACATVWIPADAPKTKLΝGMRKSΝVQKAVILPX 70 80 90 100 110 This corresponds to the amino acid sequence <SEQ ID 2008; ORF 625. a>: a625.pep 1 MFATRKMKKM TMCTRRVRFW LAFSSGRIIS IAAPVVPMIE ASAVPTASRA 51 VLSLGVPFKS PQTKMPPEMV YRASSSRMKG MYSSTSACAT VWIPADAPKT 101 KLΝGMRKSΝV QKAVILP* m625/a625 100.0% identity in 117 aa overlap 10 20 30 40 50 60 m625. pep MFATRKMKKMTMCTRRVRFWLAFSSGRIISIAAPVVPMIEASAVPTASRAVLSLGVPFKS II II II II II II II II II II I I I I I I I I I I I II I I I I I I I II I II II II II II II I II II a625 MFATRKMKKMTMCTRRVRFWLAFSSGRIISIAAPWPMIEASAVPTASRAVLΞLGVPFKS 10 20 30 40 50 60 70 80 90 100 110 m625. pep PQTKMPPEMVYRASΞSRMKGMYSSTSACATVWIPADAPKTKLΝGMRKSΝVQKAVILPX II I II II I I I II II II II I I I I I I II I I II II I I I I I I I I II II I II II II II I I I II a625 PQTKMPPEMVYRASSSRMKGMYSSTSACATVWIPADAPKTKLΝGMRKSΝVQKAVILPX 70 80 90 100 110 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 2009>: g627. seq 1 ATGTCCGGCC TTTGGAAACC CGAACACCCG GGATTTGAAA TCCTCGGCAG 51 CCGTTACGCC CTGCAAAACC TTGTCCGCGA TGTCATCCTG ATTACATTGA 101 CCGCCGTATC TATGGCAATC ACGCCCAAAC AAGTCCGCGC AGGCAACGAA 151 TTCAACTTTG AACCCATCGC CGAAGTGGGC AAACTCTTCC TCGGCATCTT 201 CATCACCATC TTCCCCGTCC TGAGCATTCT GAAAGCAGGC GAGGCAGGCG 251 CGCTGGGCGG GGTGGTATCG CTGGTTCACG ATACGGCAGG TCATCCGATT 301 AATACGATGT ATTTCTGGAT GAGCGGCATA TTGTCGGCAT TCTTGGATAA 351 CGCGCCCACT TATCTCGTGT TTTTCAATAT GGCGGGCGGC GATGCCCAAG 401 C CCCTTTTAAAATTGGAACC GGGTCCCCTG TTTCATTcgc TGCTGGCGGT TTCTAtgggT 451 tCGGTATTCA TGGGCGCACT GaccTACATc gGCAAcgcac cgaactTCAT 501 GGTcaaggcc aTTGCCGaaC agcgcgGCgt accgaTGCcg actTTCTTcc 551 ggtaTAtgat gtggtcggtc gcCTTCCTGa caCCCGTCTT CAtcgTACAT 601 ACCCTcgtCT TTTTcgTTtt cAAACTACTg taa This corresponds to the amino acid sequence <SEQ ID 2010; ORF 627.ng>: g627.pep 1 MSGLWKPEHP GFEILGSRYA LQNLVRDVIL ITLTAVSMAI TPKQVRAGNE 51 FNFEPIAEVG KLFLGIFITI FPVLSILKAG EAGALGGWS LVHDTAGHPI 101 NTMYFWMSGI LSAFLDNAPT YLVFFNMAGG DAQALMTGPL FHSLLAVSMG 151 SVFMGALTYI GNAPNFMVKA IAEQRGVPMP TFFRYMMWSV AFLTPVFIVH 201 TLVFFVFKLL * The following partial DNA sequence was identified in N meningitidis <SEQ ID 2011>: m627. seq 1 ATGTCCGGCC TTTGGAAACC CGAACACCCG GGATTTGAAA TCCTCGGCAG 51 CCGTTACGCC CTGCAAAACC TCGTCCGCGA TGTCATCCTG ATTGCATTGA 101 CCGCCGTATC TATGGCAATC ACGCCCAAAC AAGTCCGCGC AGGCAACGAA 151 TTCAACTTTG AACCCATCGC CGAAGTGGGC AAACTCTTCC TCGGCATCTT 201 CATCACCATC TTTCCCGTCC TGAGCATTCT GAAAGCAGGC GAGGCAGGCG 251 CGCTGGGCGG GGTGGTATCG CTGGTTCACG ATACGGCAGG TCATCCGATT 301 AATGTGATGT ATTTTTGGAT GAGCGGCATA TTGTCGGCAT TCTTGGATAA 351 CGCGCCCACT TATCTCGTTT TTTTCAATAT GGCGGGCGGC GATGCCCAAG 401 CCTTGATGAC GGGTACCCTG TTTCATTCGC TGCTGGCGGT TTCTATGGGT 451 TCGGTATTCA TGGGCGCACT GACCTACATC GGCAACGCAC CGAACTTCAT 501 GGTCAAGGCC ATTGCCGAAC AGCGCGGCGT ACCGATGCCG ACTTTCTTCG 551 GCTATATGAT GTGGTCGGTC GCCTTCCTGA CACCCGTCTT CATCGTACAT 601 ACCCTTATCT TTTTCGTTTT CAAACTGCTG TAA This corresponds to the amino acid sequence <SEQ ID 2012; ORF 627>: m627.pep 1 MSGLWKPEHP GFEILGSRYA LQNLVRDVIL I LTAVSMAI TPKQVRAGNE 51 FNFEPIAEVG KLFLGIFITI FPVLSILKAG EAGALGGWS LVHDTAGHPI 101 NVMYFWMSGI LSAFLDNAPT YLVFFNMAGG DAQALMTGTL FHSLLAVSMG 151 SVFMGALTYI GNAPNFMVKA IAEQRGVPMP TFFGYMMWSV AFLTPVFIVH 201 TLIFFVFKLL * Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m627/g627 97.6% identity in 210 aa overlap 10 20 30 40 50 60 m627. pep MSGLWKPEHPGFEILGSRYALQΝLVRDVILIALTAVSMAITPKQVRAGΝEFΝFEPIAEVG MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g627 MSGLWKPEHPGFEILGSRYALQΝLVRDVILITLTAVSMAITPKQVRAGΝEFΝFEPIAEVG 10 20 30 40 50 60 70 80 90 100 110 120 m627.pep KLFLGIFITIFPVLSILKAGEAGALGGWSLVHDTAGHPINVMYFWMSGILSAFLDNAPT MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g627 KLFLGIFITIFPVLSILKAGEAGALGGWSLVHDTAGHPINTMYFWMSGILSAFLDNAPT 70 80 90 100 110 120 130 140 150 160 170 180 m627. pep YLVFFNMAGGDAQALMTGTLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMP II II II I II I II II I I II II II II II II I II II II II II II II I II II II II II I I I I I g627 YLVFFNMAGGDAQALMTGPLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMP 130 140 150 160 170 180 190 200 210 m627. pep TFFGYMMWSVAFLTPVFIVHTLIFFVFKLLX III I I I I I I II II II II II I I : II II II I I g627 TFFRYMMWSVAFLTPVFIVHTLVFFVFKLLX 190 200 210 The following partial DNA sequence was identified inN meningitidis <SEQ ID 2013>: a627. seq 1 ATGTCCGGCC TTTGGAAACC CGAACACCCG GGATTTGAAA TCCTCGGCAG 51 CCGTTACGCC CTGCAAAACC TCGTCCGCGA TGTCATCCTG ATTGCATTGA 101 CCGCCGTATC TATGGCAATC ACGCCCAAAC AAGTCCGCGC AGGCAACGAA 151 TTCAACTTTG AACCCATCGC CGAAGTGGGC AAACTCTTCC TCGGCATCTT 201 CATCACCATC TTTCCCGTCC TGAGCATTCT GAAAGCAGGC GAGGCAGGCG 251 CGCTGGGCGG GGTGGTATCG CTGGTTCACG ATACGGCAGG TCATCCGATT 301 AATGTGATGT ATTTTTGGAT GAGCGGCATA TTGTCGGCAT TCTTGGATAA 351 CGCGCCCACT TATCTCGTTT TTTTCAATAT GGCGGGCGGC GATGCCCAAG 401 CCTTGATGAC GGGTTCCCTG TTTCATTCGC TGCTGGCGGT TTCTATGGGT 451 TCGGTATTCA TGGGCGCACT GACCTACATC GGCAACGCAC CGAACTTCAT 501 GGTCAAGGCC ATTGCCGAAC AGCGCGGCGT ACCGATGCCG ACTTTCTTCG 551 GCTATATGAT GTGGTCGGTC GCCTTCCTGA CACCCGTCTT CATCGTACAT 601 ACCCTTATCT TTTTCGTTTT CAAACTGCTG TAA This corresponds to the amino acid sequence <SEQ ID 2014; ORF 627. a>: a627.pep 1 MSGLWKPEHP GFEILGSRYA LQNLVRDVIL IALTAVSMAI TPKQVRAGNE 51 FNFEPIAEVG KLFLGIFITI FPVLSILKAG EAGALGGWS LVHDTAGHPI 101 NVMYFWMSGI LSAFLDNAPT YLVFFNMAGG DAQALMTGSL FHSLLAVSMG 151 SVFMGALTYI GNAPNFMVKA IAEQRGVPMP TFFGYMMWSV AFLTPVFIVH 201 TLIFFVFKLL * m627/a62 99.5% identity in 210 aa overlap 10 20 30 40 50 60 m627. pep MSGLWKPEHPGFEILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVG MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a627 MSGLWKPEHPGFEILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVG 10 20 30 40 50 60 70 80 90 100 110 120 m627. pep KLFLGIFITIFPVLSILKAGEAGALGGWSLVHDTAGHPINVMYFWMSGILSAFLDNAPT M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M a627 KLFLGIFITIFPVLSILKAGEAGALGGWΞLVHDTAGHPINVMYFWMSGILSAFLDNAPT 70 80 90 100 110 120 130 140 150 160 170 180 m627. pep YLVFFNMAGGDAQALMTGTLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMP M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M a 627 YLVFFNMAGGDAQALMTGSLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMP 130 140 150 160 170 180 190 200 210 m627.pet TFFGYMMWSVAFLTPVFIVHTLIFFVFKLLX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a627 TFFGYMMWSVAFLTPVFIVHTLIFFVFKLLX 190 200 210 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2015>: g628. seq 1 ATGTGCGTGC CACTCAAGCC GGCAGGATGC GGGCCGCCAA ATTCATGTGT 51 TTCGATATTG GCAGCATTTT CAGACGGCAC GTCTGCGCCT GCTGCTTTAC 101 ACACATGGAT TTTACGTTCG GTCAGGCGGC TCAATACCAA CAGGCCGCGT 151 TTGAAGTCTT CGGCGGCTTC TTTGATGATG ACCGTAGGGT CGGCAGCCAG 201 CGGATTGGTG TCCATCGCAT TGACGAAGAT GGCGAACGGC TCGGCATCTA 251 CGGCAGGGAT TTTGCTGAAC GGACGGGTGC GAAGCGCAGT CCATAAGCCT 301 GATTGAATCA GGTTGCGGCG CACTTTTTCG CTGCTCAATT TTGCCAGCGC 351 TTCAGGTacg TAG This corresponds to the amino acid sequence <SEQ ID 2016; ORF 628.ng>: g628.pep 1 MCVPLKPAGC GPPΝSCVSIL AAFSDGTSAP AALHTWILRS VRRLΝTΝRPR 51 LKSSAASLMM TVGSAASGLV SIALTKMAΝG SASTAGILLΝ GRVRSAVHKP 101 D*IRLRRTFS LLΝFASASGT * The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2017>: m628. seq 1 ATGTGCGTGC CACTCAAACC GGCAGGATGC GGGCCGCCGA ATTCATGTGT 51 TTCGATGTTG GCAGCATTTT CAGACGGCAC GTCTGCGCCA GCTGCCTTAC 101 AAACATGGAT TTTGCGTTCG GTCAAACGGC TCAATACCAA CAGGCCGCGT 151 TTGAAATCCT CGGCGGCTTC TTTGATAATG ACCGTAGGGT CGGCAGCCAG 201 CGGATTGGTG TCCATCGCAT TGACGAAGAT GGCGAACGGC TCGGCATCGA 251 CGGCAGGAAT TTTGCTGAAC GGACGGGTGC GCAGCGCAGT CCACAAACCG 301 GATTGGATCA GGTTGCGGCG CACTTCTTCG CCGCTTAAGT TTGCCAGCGC 351 TTCAGGTGCG TAG This corresponds to the amino acid sequence <SEQ ID 2018; ORF 628>: m628.pep 1 MCVPLKPAGC GPPΝSCVSML AAFSDGTSAP AALQTWILRS VKRLΝTΝRPR 51 LKSSAASLIM TVGSAASGLV SIALTKMAΝG SASTAGILLΝ GRVRSAVHKP 101 DWIRLRRTSS PLKFASASGA * Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m628/g628 93.3% identity in 119 aa overlap 10 20 30 40 50 60 m628. pep MCVPLKPAGCGPPΝSCVSMLAAFSDGTSAPAALQTWILRSVKRLΝTΝRPRLKSSAASLIM II I II I I II I II II II II : II II II I II I II II : II II II I : II II I II I II II II II : I g628 MCVPLKPAGCGPPΝSCVSILAAFSDGTSAPAALHTWILRSVRRLΝTΝRPRLKSSAASLMM 10 20 30 40 50 60 70 80 90 100 110 120 m628. pep TVGSAASGLVSIALTKMAΝGSASTAGILLΝGRVRSAVHKPDWIRLRRTSSPLKFASASGA II II I I II I II II II II II II I I I I I II II I II II I II II I llllll I I : I II II M g628 TVGSAASGLVSIALTKMAΝGSASTAGILLΝGRVRSAVHKPDXIRLRRTFSLLΝFASASGT 70 80 90 100 110 120 m628.pep X g628 X The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2019>: a628. seq 1 ATGTGCGTGC CACTCAAACC GGCCGGATGC GGGCCGCCGA ATTCATGTGT 51 TTCGATGTTG GCAGCATTTT CAGACGGCAC GTCTGCGCCA GCTGCCTTAC 101 ACACATGGAT TTTACGCTCG GTCAAACGGC TCAATACCAG CAAACCTCGT 151 CTGAAATCCT CGGCGGCTTC TTTGATCACA ACCACAGGGT CTGCCGCCAG 201 CGGATTGGTG TCCATCGCAT TGACGAAGAT GGCGAACGGC TCGGCATCGA 251 CGGCAGGGAT TTTGCTGAAC GGACGGGTAC GCAGCGCAGT CCACAAACCG 301 GATTGGATCA GATTGCGGCG CACTTCTTCG CCGCTTAAGT TTGCCAACGC 351 TTCGGGCGCG TAG This corresponds to the amino acid sequence <SEQ ID 2020; ORF 628. a>: a628.pep 1 MCVPLKPAGC GPPNSCVSML AAFSDGTSAP AALHTWILRS VKRLNTSKPR 51 LKSSAASLIT TTGSAASGLV SIALTKMANG SASTAGILLN GRVRSAVHKP 101 DWIRLRRTSS PLKFANASGA * m628/a628 95.0% identity in 120 aa overlap 10 20 30 40 50 60 m628.pep MCVPLKPAGCGPPNSCVSMLAAFSDGTSAPAALQTWILRSVKRLNTNRPRLKSSAASLIM II II II II I I I II II II II I II II II II I II II : II II II II II II : : II II II II I II a628 MCVPLKPAGCGPPNSCVSMLAAFSDGTSAPAALHTWILRSVKRLNTSKPRLKSSAASLIT 10 20 30 40 50 60 70 80 90 100 110 120 m628. pep TVGSAASGLVSIALTKMANGSASTAGILLNGRVRSAVHKPDWIRLRRTSSPLKFASASGA MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a628 TTGSAASGLVSIALTKMANGSASTAGILLNGRVRSAVHKPDWIRLRRTSSPLKFANASGA 70 80 90 100 110 120 m628.pep X I a628 X The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2021 > • g629 . seq 1 ATGACTGCca aacCTTTTTC CCTCAACCTG GCcaaCCTCC TGCTGCCggc 51 ggtatTGTTT GCCGTCAGcc tGtcggTCGG cattgccgaT TTCCGCTGGT 101 CGGATGTGTT TTCGCTGTCC GACAGCCAGC AAGTGATGTT CATCAGCCGC 151 CTGCCGCGCA CGTTTGcgaT TGTGTTGACG GGCgcgtcga tagcgGtggc 201 gGGGAtgatt atgcagATTC TGATGCGCAA CcgtTTTGTC GAGCCTtcta 251 tggcgGGTGC GGGCCAAAGt gcgGCTTTGG GTttgcttct gAtgtccctg 301 ctgctgcctg CcgcGccgct gccggtcaAA ATGTCGGtag Ccgccgttgc 351 CGCGCTGATC GGGATGTTGG tctTtatgct gctaatccgC Cgcctgccac 401 cgacggcgca gctgatgGTg ccgCTGGTGG Gg . ttATTTT CGGCGGCGTG 451 GttgaGGCGG TGGCGACGTT TGTCGCGTAT GAGTTTGAGA TGCTGCAAAT 501 GTTGGGCGTG TGGCAGCAGG GCGACTTTTC AAGCGTGCTG CTGGGGCGGT 551 ACGAGCTGCT TTGGATTACG GGCGGTTTGG CGGTGTTTGC CTACCTGATT 601 GCCGACCGGC TGACGATTTT GGGGCTGGGC GAGACGGTGA GCGTGAATTT 651 GGGTTTGAAC CGGACGGCGG TGTTGTGGTC GGGTTTGATT ATTGTGGCAC 701 TGATTACATC GCTGGTCATT GTAACGGTCG GCAATATTCC GTTTATCGGG 751 CTGGTCGTGC CGAATATCGT CAGCCGCCTG ATGGGCGACA GGCTGCGCCA 801 AAGCCTGCCT GCGGTCGCCC TCTTGGGCGC GTCTTTGGTT TTATTGTGCG 851 ACATTATCGG ACGCATGATT GTGTTTCCGT TTGAAATTCC GGTCTCCACG 901 GTTTTTGGTG TGTTGGGTAC GGCTTTGTTT TTGTGGCTTT TGTTGAGGAA 951 ACCCGCCTAT GCCGTCTGA This corresponds to the amino acid sequence <SEQ ID 2022; ORF 629.ng>: g629.pep 1 MTAKPFSLΝL AΝLLLPAVLF AVSLSVGIAD FRWSDVFSLS DSQQVMFISR 51 LPRTFAIVLT GASIAVAGMI MQILMRΝRFV EPSMAGAGQS AALGLLLMSL 101 LLPAAPLPVK MSVAAVAALI GMLVFMLLIR RLPPTAQLMV PLVGXIFGGV 151 VEAVATFVAY EFEMLQMLGV WQQGDFSSVL LGRYELLWIT GGLAVFAYLI 201 ADRLTILGLG ETVSVNLGLN RTAVLWSGLI IVALITSLVI VTVGNIPFIG 251 LWPNIVSRL MGDRLRQSLP AVALLGASLV LLCDIIGRMI VFPFEIPVST 301 VFGVLGTALF LWLLLRKPAY AV* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2023>: m629. seq 1 ATGACTGCCA AACCTTTTTC CCTCAACCTG ACCAACCTGC TGCTGCTGGC 51 GGTGTTGTTT GCCGTCAGCC TGTCGGTGGG CGTTGCCGAT TTCCGCTGGT 101 CTGATGTGTT TTCACTGTCC GACAGCCAGC AGGTCATGTT CATCAGCCGC 151 CTGCCGCGCA CGTTTGCGAT TGTGCTGACG GGCGCGTCGA TGGCGGTGGC 201 CGGCATGATT ATGCAGATTT TGATGCGCAA CCGTTTTGTC GAACCGTCGA 251 TGGTGGGCGC AAGCCAAAGC GCGGCTTTAG GTTTGCTGCT GATGACCCTG 301 CTGCTGCCGG CCGCGCCGCT GCCGGCGAAA ATGTCGGTTG CCGCCGTTGC 351 CGCGCTGATC GGGATGTTGG TCTTTATGCT GCTGATCCGC CGCCTGCCGC 401 CGACCGCGCA ACTGATGGTG CCTTTGGTCG GGATTATTTT CGGCGGTGTG 451 ATTGAGGCGG TAGCCACCTT TATCGCGTAT GAAAACGAAA TGCTGCAAAT 501 GCTCGGCGTG TGGCAGCAGG GCGATTTTTC GAGCGTGCTG CTGGGGCGGT 551 ACGAGCTGCT TTGGATTACG GGCGGTTTGG CGGTGTTTGC CTATCTGATT 601 GCCGACCGGC TGACGATTTT GGGGCTGGGC GAAACGGTAA GCGTGAATTT 651 GGGTTTGAAC CGGACGGCGG TGTTGTGGTC GGGTTTGATT ATTGTGGCTT 701 TGATTACGTC GCTGGTTATC GTTACGGTCG GCAATATTCC GTTTATCGGG 751 CTGGTCGTGC CGAACATCAT CAGCCGCCTG ATGGGCGACA GGTTGCGCCA 801 AAGCCTGCCT GCGGTGGCCT TGCTGGGCGC ATCTTTGGTG TTGCTGTGCG 851 ACATTATCGG ACGCGTGATT GTGTTTCCGT TTGAAATTCC GGTCTCTACG 901 GTTTTTGGTG TATTGGGTAC GGCTTTGTTT TTGTGGCTTT TGTTGAGGAA 951 ACCCGCCTAT GCCGTCTGA This corresponds to the amino acid sequence <SEQ ID 2024; ORF 629>: m629.pep 1 MTAKPFSLΝL TΝLLLLAVLF AVSLSVGVAD FRWSDVFSLS DSQQVMFISR 51 LPRTFAIVLT GASMAVAGMI MQILMRΝRFV EPSMVGASQS AALGLLLMTL 101 LLPAAPLPAK MSVAAVAALI GMLVFMLLIR RLPPTAQLMV PLVGIIFGGV 151 IEAVATFIAY EΝEMLQMLGV WQQGDFSSVL LGRYELLWIT GGLAVFAYLI 201 ADRLTILGLG ETVSVΝLGLΝ RTAVLWSGLI IVALITSLVI VTVGNIPFIG 251 LWPNIISRL MGDRLRQSLP AVALLGASLV LLCDIIGRVI VFPFEIPVST 301 VFGVLGTALF LWLLLRKPAY AV* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m629/g629 95.7% identity in 322 aa overlap 10 20 30 40 50 60 m629.pep MTAKPFSLΝLTΝLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQVMFISRLPRTFAIVLT MMMMIMMM II II I II II II : I II II II II II II II II II I I II II II I I II I g629 MTAKPFSLΝLAΝLLLPAVLFAVSLSVGIADFRWSDVFSLSDSQQVMFISRLPRTFAIVLT 10 20 30 40 50 60 70 80 90 100 110 120 m629. pep GASMAVAGMIMQILMRΝRFVEPSMVGASQSAALGLLLMTLLLPAAPLPAKMSVAAVAALI I I I : I II II I II II I I II II II II : I I : I I II II II II : II II II II M II II II I II I I g629 GASIAVAGMIMQILMRΝRFVEPSMAGAGQSAALGLLLMSLLLPAAPLPVKMSVAAVAALI 70 80 90 100 110 120 130 140 150 160 170 180 m629. pep GMLVFMLLIRRLPPTAQLMVPLVGIIFGGVIEAVATFIAYEΝEMLQMLGVWQQGDFSSVL M M M M M M M M M M M M I I I M : I I I I I M I I I I I I I I I I I I I I I I I I I I I g629 GMLVFMLLIRRLPPTAQLMVPLVGXIFGGWEAVATFVAYEFEMLQMLGVWQQGDFSSVL 130 140 150 160 170 180 190 200 210 220 230 240 m629.pep LGRYELLWITGGLAVFAYLIADRLTILGLGETVSVΝLGLΝRTAVLWSGLIIVALITSLVI II I I I I I M I I II I I II I II II I I I I I II I I I I I II II II I I I I I I I I I I I I I I I I I I I I g629 LGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLGLNRTAVLWSGLI IVALITSLVI 190 200 210 220 230 240 250 260 270 280 290 300 m629.pep VTVGNIPFIGLWPNIISRLMGDRLRQSLPAVALLGASLVLLCDIIGRVIVFPFEIPVST I II I II II II II II II : I I I II I II II II II I I I II II I II II II II I : I I I I II II I I I g629 VTVGNIPFIGLWPNIVSRLMGDRLRQSLPAVALLGASLVLLCDIIGRMIVFPFEIPVST 250 260 270 280 290 300 310 320 m629. pep VFGVLGTALFLWLLLRKPAYAVX I I I I I I II I II II II I II I I I II g629 VFGVLGTALFLWLLLRKPAYAVX 310 320 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2025>: a629.seq 1 ATGACTGCCA AACCTTTTTC CCTCAACCTG ACTAACCTCC TGCTGCTGGC 51 GGTGTTGTTT GCCGTCAGCC TGTCGGTGGG CGTTGCCGAT TTCCGCTGGT 101 CGGATGTGTT TTCGCTGTCG GACAGCCAGC AGGTTATGTT CATCAGCCGC 151 CTGCCGCGCA CGTTTGCGAT TGTGTTGACG GGCGCGTCGA TGGCGGTGGC 201 GGGGATGATT ATGCAGATTC TGATGCGTAA CCGTTTTGTC GAGCCTTCTA 251 TGGCGGGCGG GGGTCAGAGT GCGGCTTTGG GTTTGCTTCT GATGTCCCTG 301 CTGCTGCCTG CCGCGCCGCT GCCGGTCAAA ATGTCGGTTG CCGCCGTTGC 351 CGCGTTAATC GGGATGTTGG TGTTTATGAT GCTTATCCGC CGCCTGCCGC 401 CGACGGCGCA ACTGATGGTG CCTTTGGTCG GGATTATTTT CGGCGGCGTG 451 GTTGAGGCGG TGGCCACCTT TATTGCGTAT GAAAACGAAA TGCTGCAAAT 501 GCTGGGCGTG TGGCAACAGG GCGATTTTTC CGGCGTGTTG CTCGGACGGT 551 ATGAACTGTT GTGGGCAACG GGGATTTTGG CTTTGTTTGC CTATTTGATT 601 GCCGACCAGC TGACGATTTT GGGTTTGGGC GAAACGGTAA GCGTGAACTT 651 GGGGCTGAAC CGGACGGCGA TTCTGTGGTC GGGGCTGATT ATTGTGGCTT 701 TGATTACGTC GCTGGTTATC GTTACGGTCG GCAATATTCC GTTTATCGGG 751 CTGGTCGTGC CGAACATCAT CAGCCGCCTG ATAGGCGACA GGCTGCGCCA 801 AAGCCTGCCT GCGGTGGCTT TGCTGGGTGC GTCTTTGGTT TTATTGTGCG 851 ACATTATCGG ACGAGTGATT GTGTTTCCGT TTGAAATTCC GGTATCGACC 901 GTCTTCGGCG TATTGGGTAC GGCGTTGTTT TTATGGCTTT TGTTAAGGAA 951 ACCTGCTCAT GCCGTCTGA This corresponds to the amino acid sequence <SEQ ID 2026; ORF 629. a>: a629.pep 1 MTAKPFSLΝL TΝLLLLAVLF AVSLSVGVAD FRWSDVFSLS DSQQVMFISR 51 LPRTFAIVLT GASMAVAGMI MQILMRΝRFV EPSMAGAGQS AALGLLLMSL 101 LLPAAPLPVK MSVAAVAALI GMLVFMMLIR RLPPTAQLMV PLVGIIFGGV 151 VEAVATFIAY EΝEMLQMLGV WQQGDFSGVL LGRYELLWAT GILALFAYLI 201 ADQLTILGLG ETVSVΝLGLΝ RTAILWSGLI IVALITSLVI VTVGNIPFIG 251 LWPNIISRL IGDRLRQSLP AVALLGASLV LLCDIIGRVI VFPFEIPVST 301 VFGVLGTALF LWLLLRKPAH AV* m629/a629 95.7% identity in 322 aa overlap 10 20 30 40 50 60 m629.pep MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQVMFISRLPRTFAIVLT MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a629 MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQVMFISRLPRTFAIVLT 10 20 30 40 50 60 70 80 90 100 110 120 m629.pep GASMAVAGMIMQILMRNRFVEPSMVGASQSAALGLLLMTLLLPAAPLPAKMSVAAVAALI II I I I I II II II II II II II II II : I I : I II II I II II : I I II II II I : II I I II II II I a629 GASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLLPAAPLPVKMSVAAVAALI 70 80 90 100 110 120 130 140 150 160 170 180 m629.pep GMLVFMLLIRRLPPTAQLMVPLVGIIFGGVIEAVATFIAYENEMLQMLGVWQQGDFSSVL II II II : I II II II II I II II I I I I I II I I : II I I II II I I I I I II I I I I II I II II : II a629 GMLVFMMLIRRLPPTAQLMVPLVGIIFGGWEAVATFIAYENEMLQMLGVWQQGDFSGVL 130 140 150 160 170 180 190 200 210 220 230 240 m629.pep LGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLGLNRTAVLWSGLIIVALITSLVI II II II II II II : II II II I : I II II II I II I I II II II I I : II I I II II I I I II II I a 629 LGRYELLWATGILALFAYLIADQLTILGLGETVSVNLGLNRTAILWSGLIIVALITSLVI 190 200 210 220 230 240 250 260 270 280 290 300 m629.pep VTVGNIPFIGLWPNIISRLMGDRLRQSLPAVALLGASLVLLCDIIGRVIVFPFEIPVST MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a629 VTVGNIPFIGLWPNIISRLIGDRLRQSLPAVALLGASLVLLCDIIGRVIVFPFEIPVST 250 260 270 280 290 300 310 320 m629.pep VFGVLGTALFLWLLLRKPAYAVX I I II I I I II II II II II I I: II I a629 VFGVLGTALFLWLLLRKPAHAVX 310 320 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2027>: g630.seq (partial) 1 aTgatGATTT TGGTGTGGCT ggctttgttt ccccccatgt tttacggcat 51 gtacaacgtc GGCGCACAGG CATTCGGTGC CTTAACGCCC GAtttgctgc 101 aacaaagcat cgcccacgac ggcaattacg ccctcgccaa cgctttgggc 151 atcaatatgt cccccgaaGc gggcgtgtTg ggcaaaatgc tgttcgGCGC 201 GATttacttc ctgccgattt acgcgaccgt aTTTATTGTG GGcggcttct 251 ggGaagtCTT GTTCGCATCc gtACGCAAAC ACGAAATCAA CGAAGGTTTC 301 TTCGTTACTT CGATTCTGTT TGCCTTAATC GTTCCGCCCA CGCTGCCGCT 351 GTGGCAGGCG GCTTTGGGTA TTTCTTTCGG CGTTGTGGTT GCGAAAGAAG 401 TATTCGGCGG TACAGGTAAA AACTTCATGA ACCCTGCGCT GGCAGGCCGC 451 GCCTTCCTGT TCTTCGCCTA CCCCGCCAAC TTGAGCGGCG ATGCGGTTTG 501 GACGGCGGTT GACGGCTATT CCGGCGCAAC CGCGCTGGCG CAATGGGCGG 551 CACACGGTGC AGACGGCCTG AAAAACGCCG TAACCGGTCA AACCATCACT 601 TGGATGGACG CGTTTATCGG CAAACTGCCC GGCTCCATCG GCGAAGTCTC 651 CACTTTGGCA CTCTTAATCG GCGGCGCGTT TATCGTGTTT GCCCGCATCG 701 CTtcttgGCG CATTATTGCc ggCGTGATGA TCGGTatGat tGcgatgTCT 751 tcgctgatta acttcatCGg ttctgacacc aaagctatgt ttgctatgca 801 cttggtacat ggcacttggt GGAaagatGa ttAtcactca ctgtacatta 851 aa This corresponds to the amino acid sequence <SEQ ID 2028; ORF 630.ng>: g630.pep 1 MMILVWLALF PPMFYGMYΝV GAQAFGALTP DLLQQSIAHD GΝYALAΝALG 51 IΝMSPEAGVL GKMLFGAIYF LPIYATVFIV GGFWEVLFAS VRKHEIΝEGF 101 FVTSILFALI VPPTLPLWQA ALGISFGVVV AKEVFGGTGK ΝFMΝPALAGR 151 AFLFFAYPAΝ LSGDAVWTAV DGYΞGATALA QWAAHGADGL KΝAVTGQTIT 201 WMDAFIGKLP GΞIGEVSTLA LLIGGAFIVF ARIASWRIIA GVMIGMIAMS 251 SLIΝFIGΞDT KAMFAMHLVH GTWWKDDYHS LYIK.... The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2029>: m630.seq 1 ATGATGATTT TGGTGTGGCT GGCTTTGTTC CCTGCCATGT TCTACGGTAT 51 GTACAACGTC GGCGCGCAGG CATTCGGTGC GTTAACGCCT GATTTGCTGC 101 AACAAAACAT CGCCAACGAC TGGCATTACG CCTTTGCCAA CGCTTTGGGC 151 ATCAATATGT CGTCTGAAGC GGGCGTGTCG GACAAAATGC TGTTTGGCGC 201 GATTTACTTC CTGCCGATTT ACGCGACTGT ATTTGTTGTG GGCGGTTTCT 251 GGGAAGTTTT GTTCGCCACC GTGCGCAAAC ACGAAATCAA CGAAGGTTTC 301 TTCGTTACTT CGATTCTGTT TGCCTTAATC GTTCCGCCCA CGCTGCCGCT 351 GTGGCAGGCG GCTTTGGGTA TTTCTTTCGG CGTTGTGGTT GCGAAAGAAG 401 TATTCGGCGG TACAGGTAAA AACTTCATGA ACCCTGCGCT GGCAGGCCGT 451 GGTTTCCTGT TCTTCGCCTA CCCTGCCAAC TTGAGCGGCG ATGCGGTTTG 501 GACGGCGGTT GACGGCTATT CCGGCGCAAC CGCACTGGCG CAATGGGCGG 551 CACACGGTGC AGACGGCCTG AAAAACGCCG TAACCGGTCA AACCATCACT 601 TGGATGGACG CGTTTATCGG CAAACTGCCC GGCTCCATTG GCGAAGTCTC 651 CACTTTGGCA CTCTTAATCG GCGGCGCGTT TATCGTGTTT GCCCGCATCG 701 CTTCTTGGCG CATTATTGCC GGCGTGATGA TCGGTATGAT TGCGATGTCT 751 TCGCTGTTCA ACTTCATCGG TTCGGACACC AACGCTATGT TTGCTATGCC 801 TTGGTACTGG CACTTGGTGG TCGGCGGCTT CGCCATCGGT ATGCTGTTTA 851 TGGCGACCGA CCCTGTTTCC GCTTCCTTTA CCAATGTCGG CAAATGGTGG 901 TACGGCGCAC TGATCGGTGT GATGTGCGTA TTAATCCGCG TGGTCAATCC 951 GGCTTACCCC GAAGGCATGA TGTTGGCGAT TCTGTTTGCC AACCTGTTTG 1001 CCCCGATTTT CGACTATTTC GTCGCACAAG CGAACATCAA ACGCAGAAAG 1051 GCGCGCAGCA ATGGCTAA This corresponds to the amino acid sequence <SEQ ID 2030; ORF 630>: 630.pep 1 MMILVWLALF PAMFYGMYNV GAQAFGALTP DLLQQNIAND WHYAFANALG 51 INMSSEAGVS DKMLFGAIYF LPIYATVFVV GGFWEVLFAT VRKHEINEGF 101 FVTSILFALI VPPTLPLWQA ALGISFGVVV AKEVFGGTGK NFMNPALAGR 151 AFLFFAYPAN LSGDAVWTAV DGYSGATALA QWAAHGADGL KNAVTGQTIT 201 WMDAFIGKLP GSIGEVSTLA LLIGGAFIVF ARIASWRIIA GVMIGMIAMS 251 SLFNFIGΞDT NAMFAMPWYW HLVVGGFAIG MLFMATDPVS ASFTNVGKWW 301 YGALIGVMCV LIRVVNPAYP EGMMLAILFA NLFAPIFDYF VAQANIKRRK 351 ARSNG* m630/g630 93.5% identity in 275 aa overlap 10 20 30 40 50 60 m630.pep MMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQNIANDWHYAFANALGINMSSEAGVS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I : I : I I : I I I I I I I I I l l l l g630 MMILVWLALFPPMFYGMYNVGAQAFGALTPDLLQQSIAHDGNYALANALGINMSPEAGVL 10 20 30 40 50 60 70 80 90 100 110 120 m630.pep DKMLFGAIYFLPIYATVFVVGGFWEVLFATVRKHEINEGFFVTSILFALIVPPTLPLWQA I I I I I I I I I I I I I I I I I : I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g630 GKMLFGAIYFLPIYATVFIVGGFWEVLFASVRKHEINEGFFVTSILFALIVPPTLPLWQA 70 80 90 100 110 120 130 140 150 160 170 180 m630. pep ALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSGDAVWTAVDGYSGATALA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g630 ALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSGDAVWTAVDGYSGATALA 130 140 150 160 170 180 190 200 210 220 230 240 m630.pep QWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLALLIGGAFIVFARIASWRIIA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g630 QWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLALLIGGAFIVFARIASWRIIA 190 200 210 220 230 240 250 260 270 280 290 300 m630.pep GVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAIGMLFMATDPVSASFTNVGKWW I I I I I I I I I I I I M I I I I I I : I I I I I III I g630 GVMIGMIAMSSLINFIGSDTKAMFAM HLVHGTWWKDDYHSLYIK . 250 260 270 280 310 320 330 340 350 m630.pep YGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIFDYFVAQANIKRRKARSNGX The following partial DNA sequence was identified in N meningitidis <SEQ ID 2031>: a630. seq 1 ATGATGATTT TGGTGTGGCT GGCTTTGTTC CCTGCCATGT TCTACGGTAT 51 GTACAACGTC GGCGCACAGG CATTCGGTGC GTTAACGCCC GATTTGCTGC 101 AACAAAGCAT CGCCAACGAC TGGCATTACG CCCTTGCCAA CGCTTTGGGC 151 ATCAATATGT CGTCTGAAGC GGGCGTGTTG GGCAAAATGC TGTTCGGCGC 201 GATTTACTTC CTGCCGATTT ACGCGACCGT ATTTATTGTC GGCGGTTTCT 251 GGGAAGTTTT GTTCGCCACC GTGCGCAAAC ATGAAATCAA CGAAGGTTTC 301 TTTGTTACCT CGATTCTGTT TGCCTTAATC GTTCCGCCCA CGCTGCCGCT 351 GTGGCAGGCA GCTTTGGGTA TTTCTTTCGG CGTTGTGGTT GCGAAAGAAG 401 TATTCGGCGG TACAGGTAAA AACTTCATGA ACCCTGCGCT GGCAGGCCGT 451 GCCTTCCTGT TCTTCGCCTA CCCTGCCAAC TTGAGCGGCG ATGCGGTTTG 501 GACGGCGGTT GACGGCTATT CCGGCGCAAC CGCGCTGGCG CAATGGGCGG 551 CACACGGTGC AGACGGCCTG AAAAACGCCA TAACCGGTCA AACCATCACT 601 TGGATGGATG CGTTTATCGG CAAACTGCCC GGCTCCATCG GCGAAGTCTC 651 CACTTTGGCA CTCTTAATCG GCGGCGCGTT TATCGTGTTT GCCCGCATCG 701 CTTCTTGGCG CATTATTGCC GGCGTGATGA TCGGTATGAT TGCCATGTCT 751 TCGCTGTTCA ACTTCATCGG TTCGGACACC AACGCTATGT TTGCTATGCC 801 TTGGTACTGG CATTTGGTCG TCGGCGGCTT CGCCATCGGT ATGCTGTTTA 851 TGGCGACCGA CCCCGTTTCC GCTTCCTTTA CCAATGTCGG CAAATGGTGG 901 TACGGCGCAC TGATCGGTGT GATGTGCGTA TTAATCCGCG TGGTCAATCC 951 GGCTTACCCC GAAGGCATGA TGTTGGCGAT TCTGTTTGCC AACCTGTTTG 1001 CCCCGATTTT CGACTATTTC GTCGCACAAG CGAACATCAA ACGCAGAAAG 1051 GCGCGCAGCA ATGGCTAA This corresponds to the amino acid sequence <SEQ ID 2032; ORF 630. a>: a630.pep 1 MMILVWLALF PAMFYGMYNV GAQAFGALTP DLLQQSIAND WHYALANALG 51 INMSSEAGVL GKMLFGAIYF LPIYATVFIV GGFWEVLFAT VRKHEINEGF 101 FVTSILFALI VPPTLPLWQA ALGISFGVW AKEVFGGTGK NFMNPALAGR 151 AFLFFAYPAN LSGDAVWTAV DGYSGATALA QWAAHGADGL KNAITGQTIT 201 WMDAFIGKLP GSIGEVSTLA LLIGGAFIVF ARIASWRIIA GVMIGMIAMS 251 SLFNFIGSDT NAMFAMPWYW HLWGGFAIG MLFMATDPVS ASFTNVGKWW 301 YGALIGVMCV LIRWNPAYP EGMMLAILFA NLFAPIFDYF VAQANIKRRK 351 ARSNG* m630/a630 98.3% identity in 355 aa overlap 10 20 30 40 50 60 m630. pep MMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQNIANDWHYAFANALGINMSSEAGVS II II I I II II I I II I I I I II II I II I I I II I I II I : II I II II I : II I II II II I I I II a630 MMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYALANALGINMSSEAGVL 10 20 30 40 50 60 70 80 90 100 110 120 m630. pep DKMLFGAIYFLPIYATVFWGGFWEVLFATVRKHEINEGFFVTSILFALIVPPTLPLWQA II II II II II II II II I : I II II II I II II I II II I II II I II I II II II II II II II I a630 GKMLFGAIYFLPIYATVFIVGGFWEVLFATVRKHEINEGFFVTSILFALIVPPTLPLWQA 70 80 90 100 110 120 130 140 150 160 170 180 m630. pep ALGISFGWVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSGDAVWTAVDGYSGATALA MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a630 ALGISFGWVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSGDAVWTAVDGYSGATALA 130 140 150 160 170 180 190 200 210 220 230 240 m630. pep QWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLALLIGGAFIVFARIASWRIIA MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a630 QWAAHGADGLKNAITGQTITWMDAFIGKLPGSIGEVSTLALLIGGAFIVFARIASWRIIA 190 200 210 220 230 240 250 260 270 280 290 300 m630. pep GVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAIGMLFMATDPVSASFTNVGKWW II II I I I II II II I I II I II I I II II I I II I I II II II I I II II II II II II I II II II I a630 GVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAIGMLFMATDPVSASFTNVGKWW 250 260 270 280 290 300 310 320 330 340 350 m630. pep YGALIGVMCVLIRWNPAYPEGMMLAILFANLFAPIFDYFVAQANIKRRKARSNGX II II I II II II II II II I I II II II I II II II II II II II II I II I II II I II I II a630 YGALIGVMCVLIRWNPAYPEGMMLAILFANLFAPIFDYFVAQANIKRRKARSNGX 310 320 330 340 350 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2033>: g635. seq 1 ATGACCCGGC GACGGGTCGG CAAGCAAAAC CGTATTGCCA TCCACTCCGC 51 GCAATACCGA AAAATGGTCG TCTTTGCGGT ATTTCAGATA CACGATGACG 101 GGGATTTTCA ACTGCGCGAG CTGTTCGAAA GACAGGGCAT AGCCTTTCGC 151 CTCAAAACCC AAATCGGGCA TAATGCGCCG CATATCCTCA AACGACGCGC 201 GCATCTGTTC CTTACCCAGT TTTTCCAACA CTTCTTCTTC CGTCAGCTTT 251 TGCCCGTAAA AATTGTTCAA AAGCGTCGCC ACCGAAGCCG CCCCGCAGGA 301 AAAATCCAAA TCCTGCTTTA CAATATTGAA ATCCCGCCGC GCTTTCCAAC 351 TCTGCAATTT GATTTTTCCG TAAACAACAG GATTATCGTT AAACATCGGT 401 GCAGCATTCA AACGATAAGA CAAGGGTCTG TACCAGATTA G This corresponds to the amino acid sequence <SEQ ID 2034; ORF 635.ng>: g635.pep 1 MTRRRVGKQN RIAIHΞAQYR KMVVFAVFQI HDDGDFQLRE LFERQGIAFR 51 LKTQIGHNAP HILKRRAHLF LTQFFQHFFF RQLLPVKIVQ KRRHRSRPAG 101 KIQILLYNIE IPPRFPTLQF DFSVNNRIIV KHRCSIQTIR QGSVPD* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2035>: 635. seq 1 ATGACCCAGC GACGGGTCGG CAAGCAAAAC CGTATTGCCG TCTATACCGC 51 GCAATACCGA GAAATGATCA TCCTTGCGGT ATTTCAGATA CACGATGACG 101 GGGATTTGCA ACTGTGCAAG CTGCTCGAAA GACAGGGCAT AGCCTTTCGC 151 TTCAAAACCC AAATCAGGCA TAATGCGCCG CATATCCTCA AACGACGCGG 201 GCATCTGCTC CTTATCCAGT TTTTTTAACA CGTCCTCTTC CGTCAGCTTT 251 TGCCCGTAAA AATTGTTCAA AAGCGTCACC ACCGAAGCCG CCCCGCAGGA 301 AAAATCCAAA TCCTGCTTTA CAATATTGAA ATCGCGCCTT TCTTTCCAAC 351 TCTGCACTTT GATTTTTCCA TAAGCAACAG GATTATAGTG GATTAA This corresponds to the amino acid sequence <SEQ ID 2036; ORF 635>: m635.pep 1 MTQRRVGKQΝ RIAVYTAQYR EMIILAVFQI HDDGDLQLCK LLERQGIAFR 51 FKTQIRHΝAP HILKRRGHLL LIQFF*HVLF RQLLPVKIVQ KRHHRSRPAG 101 KIQILLYNIE IAPFFPTLHF DFSISNRIIV D* 635/g635 80.0% identity in 130 aa overlap 10 20 30 40 50 60 m635.pep MTQRRVGKQNRIAVYTAQYREMIILAVFQIHDDGDLQLCKLLERQGIAFRFKTQIRHNAP I I : I I I I I I I I I I :::l I I M I ::: I I I I I I I I I M I I : I : I I I I I I I I : I I I I l l l l g635 MTRRRVGKQNRIAIHSAQYRKMVVFAVFQIHDDGDFQLRELFERQGIAFRLKTQIGHNAP 10 20 30 40 50 60 70 80 90 100 110 120 m635.pep HILKRRGHLLLIQFFXHVLFRQLLPVKIVQKRHHRSRPAGKIQILLYNIEIAPFFPTLHF I I I I I l I M I II I : I I I I I I I I I I I I I: I I I I I I I I I I I I I I I I I I I M UM g635 HILKRRAHLFLTQFFQHFFFRQLLPVKIVQKRRHRSRPAGKIQILLYNIElPPRFPTLQF 70 80 90 100 110 120 130 m635.pep DFSISNRIIVDX I I I :: I I I I I g635 DFSVNNRIIVKHRCSIQTIRQGSVPDX 130 140 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2037>: a635. seq 1 ATGACCCAGC GACGGGTCGG CAAGCAAAAC CGTATTGCCG TCTATACCGC 51 GCAATACCGA GAAATGATCA TCCTTGCGGT ATTTCAGATA CACGATGACG 101 GGGATTTGCA ACTGTGCAAG CTGCTCGAAA GACAGGGCAT AGCCTTTCGC 151 CTCAAAACCC AAATCAGGCA TGATGCGCCG CATATCCTCA AACGACGCGC 201 GCATCTGCTC CTTATCCAGC TTTTTCAACA CGTCCTCTTC CGTCAGCTTT 251 TGCCCGTGAA AATTGTTCAA AAGCGTCGCC ACCGAAGCCG CCCCGCAGGA 301 AAAATCCAAA TCCTGCTTTA CAATATTGAA ATCGCGCCTT TCTTTCCAAC 351 TCTGCACTTT GATTTTTCCA TAAGCAACAG GATTATAGTG GATTAA This corresponds to the amino acid sequence <SEQ ID 2038; ORF 635.a>: a635.pep 1 MTQRRVGKQΝ RIAVYTAQYR EMIILAVFQI HDDGDLQLCK LLERQGIAFR 51 LKTQIRHDAP HILKRRAHLL LIQLFQHVLF RQLLPVKIVQ KRRHRSRPAG 101 KIQILLYNIE IAPFFPTLHF DFSISNRIIV D* m635/a635 95.4% identity in 131 aa overlap 10 20 30 40 50 60 m635 . pep MTQRRVGKQNRIAVYTAQYREMIILAVFQIHDDGDLQLCKLLERQGIAFRFKTQIRHNAP M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M a635 MTQRRVGKQNRIAVYTAQYREMIILAVFQIHDDGDLQLCKLLERQGIAFRLKTQIRHDAP 10 20 30 40 50 60 70 80 90 100 110 120 m635. pep HILKRRGHLLLIQFFXHVLFRQLLPVKIVQKRHHRSRPAGKIQILLYNIEIAPFFPTLHF MMMMIMMM II II II I II I II I II I : II II II II I I II I I I II I II II I I I II a635 HILKRRAHLLLIQLFQHVLFRQLLPVKIVQKRRHRSRPAGKIQILLYNIEIAPFFPTLHF 70 80 90 100 110 120 130 m635.pep DFSISNRIIVDX I I I I II I I I II I a635 DFSISNRIIVDX 130 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2039>: g638.seq 1 ATGATTGGCG GACAGTTTAT CGTAGttgGc atTGTAGGCA AAAACGCACT 51 TGCCCGCTTT GTTGATAATA ttgtcGTGAA TAtcGGAATA GTTGACATAG 101 TTGAGCATGA TGCCCTAATC GCGGCTGCCG ACGGCGATAT TGTCGAACAC 151 TTTGAGCGGT TCGGAAAACA TCAGCACATA GCCCATATTG TtgcCCACGG 201 AAATATTGCC GCTGacttcg ctgtcgTTGG TGTACATATA GTGGACGGCG 251 AAACGCAGGT CGCTGAAGCG GTTGTTTTTA TAGGTGTTGT GCGTGCTGGT 301 ATTGGAAAAA ATGCCGTCCC GCCCTTTGGA AATGTCGTTG ccgACGACCT 351 GCGCgccggg CgcgtTCCAA ACGGTAACGC CATTGCCGCG CTCATTCACG 401 CGCAAGGTcg catcgCCGAC GATTTTATTC TCGCGCACCA TCGCATCGGC 451 AGAACCATGA AGGTATACGC CGAACGAATT ATCAAAAATA TTGTTGTGTT 501 CAACCAGGGC GCGCGGGGCG GCTTTTTCGA GATAAATACC GGCATCCATT 551 GCTGGCAGGC TCATACCGGA ACGGGTAACG GTCAGGTTGC GGAGCGTTAC 601 GTCCGGCGCG TGTACGGCTA TGGTACGCCC GCTCTTGTCC CCTTCGATGG 651 TTGCGGAACG GTCGGCAGGC CCTTCAATCG TAATCGGTTT GTCGATATAA 701 AGTTTGGTTT GATATACGCC GGAAGCCAGT TTGATCGTAT CGCCCGCCCG 751 GGCGCGGGCA AAAATTTCGG CAAGGTTGTC TTGCGGGGAA ACGTGGACGA 801 CGGCTGCCGA TGCCGTCTGA AAAATGCTGC CGGCGGCAAG TATCAGCACG 851 GCCTTCAGCC ATATACGGAG CGCGGATGTG TGCATAGTGT CCCTCTGTTT 901 CGTTCGGTAT GGCCGAACAA AATAAAGCAT CATTCAAATG TGCCTGTTTT 951 TATAGCGAAA CCGCCTGAAA CGGTACGGCA AGCGGTTTGG CTATAA This corresponds to the amino acid sequence <SEQ ID 2040; ORF 638.ng>: g638.pep 1 MIGGQFIVVG IVGKΝALARF VDΝIVVΝIGI VDIVEHDALI AAADGDIVEH 51 FEPFGKHQHI AHIVAHGΝIA ADFAVVGVHI VDGETQVAEA VVFIGVVRAG 101 IGKΝAVPPFG ΝVVADDLRAG RVPΝGΝAIAA LIHAQGRIAD DFILAHHRIG 151 RTMKVYAERI IKΝIVVFΝQG ARGGFFEIΝT GIHCWQAHTG TGΝGQVAERY 201 VRRVYGYGTP ALVPFDGCGT VGRPFΝRΝRF VDIKFGLIYA GSQFDRIARP 251 GAGKΝFGKVV LRGΝVDDGCR CRLKΝAAGGK YQHGLQPYTE RGCVHSVPLF 301 RSVWPΝKIKH HSΝVPVFIAK PPETVRQAVW L* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2041>: m638.seq 1 ATGATTGGCG AAAAGTTTAT CGTAGTTGGC ATTATAGGCA AATACGCACT 51 TGCCTGCCTT GTTGATAATG TTGTCGTGAA TATCGGAATA GTTGACATAG 101 TTGAGCATAA TGCCCTGATC GCGGCTGCCG ACGGCGATAT TGTCGAATAC 151 TTTGAGCCGC TCGGAAAACA TCAGCACATA GCCCATATTG TTGCCCACGG 201 AAATATTGCC GCTGATTTCG CTGTCGTTGG TGTACATATA GTGGACGGCG 251 AAACGCAAAT CGCTGAAGCG GTTGTTTTTG TAGGTGTTGT GCGTGCTGGT 301 ATTGGAAAAA ATGCCGTCCC GCCCTTTGGA AATGTCGTTG CCGACGACCT 351 GCGCACCGGG TGCGTTCCAA ACGGTAACGC CGTTGCCGCG CTCGTTCACG 401 CGCAAAGTCG CGTCGCCGAC GATTTTATTC TCGCGCACCA TCGCATCGGC 451 AGAACCATGC AGATATACGC CGACCGAATT ATCCAAAATA TTGTTGTGTT 501 CAATCAGGGC GCGCGGGGCA GTTTCTTCGA GATAAATACC GGCATCCATT 551 GCGGGCAGGC TCATACCGGA ACGGGTAACG GTCAGGTTGC GGAGCGTTAC 601 GTCCGGCGCG TGTACGGCTA TGGTACGCCC GCTCCTGTCG CCTTCGATGG 651 TTGCGGAACG GTCGGCAGGC CCTTCAATCG TAATCGGTTT GTCAATGTGA 701 AGTTTGGTTT TATATACGCC GGAAGCCAGT TTGAGCGTAT CGCCCGCCCG 751 GGCGCGGGCA AATGCGGGAT ACCGATCAGC ATAATCGGTT CGTGA This corresponds to the amino acid sequence <SEQ ID 2042; ORF 638>: m638.pep 1 MIGEKFIVVG IIGKYALACL VDNVVVNIGI VDIVEHNALI AAADGDIVEY 51 FEPLGKHQHI AHIVAHGNIA ADFAVVGVHI VDGETQIAEA VVFVGVVRAG 101 IGKNAVPPFG NVVADDLRTG CVPNGNAVAA LVHAQSRVAD DFILAHHRIG 151 RTMQIYADRI IQNIVVFNQG ARGSFFEINT GIHCGQAHTG TGNGQVAERY 201 VRRVYGYGTP APVAFDGCGT VGRPFNRNRF VNVKFGFIYA GSQFERIARP 251 GAGKCGIPIS IIGS* m638/g638 88.2% identity in 254 aa overlap 10 20 30 40 50 60 m638.pep MIGEKFIVVGIIGKYALACLVDNVVVNIGIVDIVEHNALIAAADGDIVEYFEPLGKHQHI III : I I I I I I : I I III : I I I : I I I I I I I I I I I I : I I I I I I I I I I I M I I I : I I I I I I g638 MIGGQFIVVGIVGKNALARFVDNIVVNIGIVDIVEHDALIAAADGDIVEHFEPFGKHQHI 10 20 30 40 50 60 70 80 90 100 110 120 m638.pep AHIVAHGNIAADFAVVGVHIVDGETQIAEAVVFVGVVRAGIGKNAVPPFGNVVADDLRTG I I I I I I I I I I I I I II I I I I I I I I I I I : I I I I I I : I I I I I I I I I I I I I I I I II I I I I I I : I g638 AHIVAHGNIAADFAVVGVHIVDGETQVAEAVVFIGVVRAGIGKNAVPPFGNVVADDLRAG 70 80 90 100 110 120 130 140 150 160 170 180 m638.pep CVPNGNAVAALVHAQSRVADDFILAHHRIGRTMQIYADRIIQNIVVFNQGARGSFFEINT I II I I I : I I I : I I I : I : I I I I I I I I I I I I I I I :: I I :| I I : I I I I I I I I I I M I I I I I I g638 RVPNGNAIAALIHAQGRIADDFILAHHRIGRTMKVYAERIIKNIVVFNQGARGGFFEINT 130 140 150 160 170 180 190 200 210 220 230 240 m638.pep GIHCGQAHTGTGNGQVAERYVRRVYGYGTPAPVAFDGCGTVGRPFNRNRFVNVKFGFIYA llll I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : : I I I : I I I g638 GIHCWQAHTGTGNGQVAERYVRRVYGYGTPALVPFDGCGTVGRPFNRNRFVDIKFGLIYA 190 200 210 220 230 240 250 260 m638.pep GSQFERIARPGAGKCGIPISIIGΞX I I I I : I I I I I I I I I g638 GSQFDRIARPGAGKNFGKVVLRGNVDDGCRCRLKNAAGGKYQHGLQPYTERGCVHSVPLF 250 260 270 280 290 300 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2043>: a638. seq 1 ATGATTGGCG GACAGTTTAT CGTAGTTGGC ATTGTAGGCA AAAACGCACT 51 TGCCCGCTTT GTTGATAATG TTGTCGTGAA TATCGGAATA GTTGACATAG 101 TTGAGCATGA TGCCTTGGTC GCGGCTGCCG ACGGCGATAT TGTCAAACAC 151 TTTGAGCCGC TCGGAAAACA TCAGCACATA GCCCATATTG TTGCCCACGG 201 AAATATTGCC GCTGATTTCG CTGTCGTTGG TGTACATATA GTGGACGGCG 251 AAACGCAAAT CGCTGAAGCG GTTGTTTTTA TAGGTGTTGT GCGTGCTGGT 301 ATTGGAAAAA ATGCCGTCCC GCCCTTTGGA AATATCGTTG CCGACGACCT 351 GCGCGCCGGG CGCGTTCCAA ACGGTAACGC CATTGCCGCG CTCGTTCACG 401 CGCAAAGTCG CGTCGCCGAC GATTTTATTC TCCCGCACCA TCGCATCGGC 451 AGAACCATGC AGATAGACGC CGACCGAATT ATCCAAAATA TTATTGTGTT 501 CAATCAGGGC GCGCGGGGCA GTTTCTTCGA GATAAATACC GGCATCCATT 551 GCGGGCAGGC TCATACCGGA ACGGGTAACG GTCAGGTTGC GGAGCGTTAC 601 GTCCGGCGCG TGTACGGCTA TGGTACGCCC GCTCCTGTCT CCTTCGATGG 651 TTGCAGAACG GTCGGCAGGC CCTTCAATCG TAATCGGTTT GTCGATGTGA 701 AGTTTGGTTT GATATACGCC GGAAGCCAGT TTGAGCGTAT CGCCCGCCCG 751 GGCGCGGGCA AATGCGGGAT ACCGATCAGC ATAATCGACT CATGGTGA This corresponds to the amino acid sequence <SEQ ID 2044; ORF 638.a>: a638.pep i MIGGQFIVVG IVGKΝALARF VDΝWVΝIGI VDIVEHDALV AAADGDIVKH 51 FEPLGKHQHI AHIVAHGΝIA ADFAVVGVHI VDGETQIAEA WFIGWRAG 101 IGKΝAVPPFG ΝIVADDLRAG RVPΝGΝAIAA LVHAQSRVAD DFILPHHRIG 151 RTMQIDADRI IQΝIIVFΝQG ARGSFFEINT GIHCGQAHTG TGNGQVAERY 201 VRRVYGYGTP APVSFDGCRT VGRPFNRNRF VDVKFGLIYA GSQFERIARP 251 GAGKCGIPIS IIDSW* m638/a638 91.3% identity in 264 aa overlap 10 20 30 40 50 60 m638.pep MIGEKFIWGIIGKYALACLVDNVWNIGIVDIVEHNALIAAADGDIVEYFEPLGKHQHI III : II I I I I : II III : I I I II II II I II II I I : I I : I II II II I : : I II II II II I a638 MIGGQFIWGIVGKNALARFVDNVWNIGIVDIVEHDALVAAADGDIVKHFEPLGKHQHI 10 20 30 40 50 60 70 80 90 100 110 120 m638 . pep AHIVAHGNIAADFAWGVHIVDGETQIAEAWFVGWRAGIGKNAVPPFGNVVADDLRTG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I : I I I I I I : I a638 AHIVAHGNIAADFAWGVHIVDGETQIAEAVVFIGWRAGIGKNAVPPFGNIVADDLRAG 70 80 90 100 110 120 130 140 150 160 170 180 m638 . pep CVPNGNAVAALVHAQSRVADDFILAHHRIGRTMQIYADRIIQNIWFNQGARGSFFEINT I I I I I I : I I I I I I I I I I I I I I I I l l l l l l l l l l M M M M M M M M M M M M a638 RVPNGNAIAALVHAQSRVADDFILPHHRIGRTMQIDADRIIQNIIVFNQGARGSFFEINT 130 140 150 160 170 180 190 200 210 220 230 240 m638. pep GIHCGQAHTGTGNGQVAERYVRRVYGYGTPAPVAFDGCGTVGRPFNRNRFVNVKFGFIYA II I II I I I II II I II I I II I II II I II II II I I : I II I I II II II II II I : I I I I: I II a638 GIHCGQAHTGTGNGQVAERYVRRVYGYGTPAPVSFDGCRTVGRPFNRNRFVDVKFGLIYA 190 200 210 220 230 240 250 260 m638. pep GSQFERIARPGAGKCGIPISIIGSX II I II II II II II II II II II I I a638 GSQFERIARPGAGKCGIPISIIDSWX 250 260 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2045>: g639-l.seq 1 ATGAGCCTGC CAGCAATGGA TGCCGGTATT TATCTCGAAA AAGCCGCCCC 51 GCGCGCCCTG GTTGAACACA ACAATATTTT TGATAATTCG TTCGGCGTAT 101 ACCTTCATGG TTCTGCCGAT GCGATGGTGC GCGAGAATAA AATCGTCGGC 151 GATGCGACCT TGCGCGTGAA TGAGCGCGGC AATGGCGTTA CCGTTTGGAA 201 CGCGCCCGGC GCGCAGGTCG TCGGCAACGA CATTTCCAAA GGGCGGGACG 251 GCATTTTTTC CAATACCAGC ACGCACAACA CCTATAAAAA CAACCGCTTC 301 AGCGACCTGC GTTTCGCCGT CCACTATATG TACACCAACG ACAGCGAAGT 351 CAGCGGCAAT ATTTCCGTGG GCAACAATAT GGGCTATGTG CTGATGTTTT 401 CCGAACGGCT CAAAGTGTTC GACAATATCG CCGTCGGCAG CCGCGATTAG 451 GGCATCATGC TCAACTATGT CAACTATTCC GATATTCACG ACAATATTAT 501 CAACAAAGCG GGCAAGTGCG TTTTTGCCTA CAATGCCAAC TACGATAAAC 551 TGTCCGCCAA TCATTTTGAA AACTGCCAAA TCGGCATGCA CTTTACCGCC 601 GCCATCGAAG GCACGTCCCT GCACGACAAT TCCTTTATCA ACAACGGAAG 651 CCAGGTCAAA TATGTCAGTA CGCGCTTTCT CGACTGGAGC GAGGGCGGAC 701 ACGGCAACTA CTGGAGCGAC AACAGCCCGT TCGATTTGAA CGGCGACGGC 751 TTCGGAGACA GCGCGTACCG TCCCGACGGC ATCATCGACC AAATCATCTG 801 GCGCGCGCCC GTATCGCGCC TCTTGATGAA CAGTCCCGCA ATCAGCATCG 851 TCAAATGGGC GCAGGCGCAG TTTCCCGCCG TTCTGCCCGG CGGCGTGGTG 901 GACAGCAAAC CGCTGATGAA GCCTTATGCC CCCAAAATTC AAACCCGTTA 951 TCAGGCGATG AAGGACGAGT TGCTCAAAGA AGCCGAAACG CGGCAGTCGG 1001 AACGGGGCAG GGCGGAAAAC GGTTCTTTGA ACTAG This corresponds to the amino acid sequence <SEQ ID 2046; ORF 639-l.ng>: g639-l.pep 1 MSLPAMDAGI YLEKAAPRAL VEHΝΝIFDΝS FGVYLHGSAD AMVREΝKIVG 51 DATLRVΝERG ΝGVTVWΝAPG AQVVGΝDISK GRDGIFSΝTS THΝTYKΝΝRF 101 SDLRFAVHYM YTΝDSEVSGΝ ISVGΝΝMGYV LMFSERLKVF DΝIAVGSRD* 151 GIMLΝYVΝYS DIHDΝIIΝKA GKCVFAYΝAΝ YDKLSAΝHFE ΝCQIGMHFTA 201 AIEGTSLHDΝ SFIΝΝGSQVK YVSTRFLDWS EGGHGΝYWSD ΝSPFDLΝGDG 251 FGDSAYRPDG IIDQIIWRAP VSRLLMΝSPA ISIVKWAQAQ FPAVLPGGVV 301 DSKPLMKPYA PKIQTRYQAM KDELLKEAET RQSERGRAEΝ GSLΝ* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2047>: m639-l . seq 1 ATGAGCCTGC CCGCAATGGA TGCCGGTATT TATCTCGAAG AAACTGCCCC 51 GCGCGCCCTG ATTGAACACA ACAATATTTT GGATAATTCG GTCGGCGTAT 101 ATCTGCATGG TTCTGCCGAT GCGATGGTGC GCGAGAATAA AATCGTCGGC 151 GACGCGACTT TGCGCGTGAA CGAGCGCGGC AACGGCGTTA CCGTTTGGAA 201 CGCACCCGGT GCGCAGGTCG TCGGCAACGA CATTTCCAAA GGGCGGGACG 251 GCATTTTTTC CAATACCAGC ACGCACAACA CCTACAAAAA CAACCGCTTC 301 AGCGATTTGC GTTTCGCCGT CCACTATATG TACACCAACG ACAGCGAAAT 351 CAGCGGCAAT ATTTCCGTGG GCAACAATAT GGGCTATGTG CTGATGTTTT 401 CCGAGCGGCT CAAAGTATTC GACAATATCG CCGTCGGCAG CCGCGATCAG 451 GGCATTATGC TCAACTATGT CAACTATTCC GATATTCACG ACAACATTAT 501 CAACAAGGCA GGCAAGTGCG TATTTGCCTA TAATGCCAAC TACGATAAAC 551 TTTTCGCCAA TCATTTTGAA AACTGTCAAA TCGGCATACA CTTTACCGCC 601 GCCATCGAAG GCACGTCCTT GCATGACAAT TCCTTTATCA ACAACGAAAG 651 CCAGGTCAAA TACGTCAGCA CGCGCTTTCT CGATTGGAGC GAGGGCGGAC 701 ACGGCAACTA TTGGAGCGAC AACAGCGCGT TCGATTTGAA CGGCGACGGC 751 TTCGGAGACA GCGCGTACCG CCCCAACGGC ATCATCGACC AAATCATCTG 801 GCGCGCGCCC GTATCGCGCC TTTTGATGAA CAGTCCCGCA ATCAGCATCG 851 TCAAATGGGC GCAGGCGCAG TTTCCCGCCG TTCTGCCTGG CGGCGTGGTG 901 GACAGCAAAC CGCTGATGAA GCCTTATGCC CCCAAAATTC AAACCCGTTA 951 TCAGGCGATG AAGGACGAGC TACTCAAAGA AGTCGAAACG CGGCAGTCGG 1001 AATGGGGCAG GGCGGAAAAC GGTTCTTTGA ACTAG This corresponds to the amino acid sequence <SEQ ID 2048; ORF 639-l>: m639-l.pep 1 MSLPAMDAGI YLEETAPRAL IEHNNILDNS VGVYLHGSAD AMVRENKIVG 51 DATLRVNERG NGVTVWNAPG AQVVGNDISK GRDGIFSNTS THNTYKNNRF 101 SDLRFAVHYM YTNDSEISGN ISVGNNMGYV LMFSERLKVF DNIAVGSRDQ 151 GIMLNYVNYS DIHDNIINKA GKCVFAYNAN YDKLFANHFE NCQIGIHFTA 201 AIEGTSLHDN SFINNESQVK YVSTRFLDWS EGGHGNYWSD NΞAFDLNGDG 251 FGDSAYRPNG IIDQIIWRAP VSRLLMNSPA ISIVKWAQAQ FPAVLPGGVV 301 DSKPLMKPYA PKIQTRYQAM KDELLKEVET RQΞEWGRAEN GSLN* g639-l/m639-l 95.9% identity in 344 aa overlap 10 20 30 40 50 60 g639-l.pep MSLPAMDAGIYLEKAAPRALVEHNNIFDNSFGVYLHGSADAMVRENKIVGDATLRVNERG I I I I I I I I I I I I I :: I I I I M I I I I M I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m639-l MSLPAMDAGIYLEETAPRALIEHNNILDNSVGVYLHGSADAMVRENKIVGDATLRVNERG 10 20 30 40 50 60 70 80 90 100 110 120 g639-l.pep NGVTVWNAPGAQWGNDISKGRDGIFSNTSTHNTYKNNRFSDLRFAVHYMYTNDSEVSGN I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I m639- l NGVTVWNAPGAQWGNDISKGRDGIFSNTSTHNTYKNNRFΞDLRFAVHYMYTNDSEISGN 70 80 90 100 110 120 130 140 150 160 170 180 g639- l . pep ISVGNNMGYVLMFSERLKVFDNIAVGSRDXGIMLNYVNYSDIHDNIINKAGKCVFAYNAN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II1639-1 ISVGNNMGYVLMFSERLKVFDNIAVGSRDQGIMLNYVNYSDIHDNIINKAGKCVFAYNAN 130 140 150 160 170 180 190 200 210 220 230 240 g639-l.pep YDKLSANHFENCQIGMHFTAAIEGTSLHDNSFINNGSQVKYVSTRFLDWSEGGHGNYWSD llll I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m639-l YDKLFANHFENCQIGIHFTAAIEGTSLHDNSFINNESQVKYVSTRFLDWSEGGHGNYWSD 190 200 210 220 230 240 250 260 270 280 290 300 g639-l.pep NSPFDLNGDGFGDSAYRPDGIIDQIIWRAPVSRLLMNSPAISIVKWAQAQFPAVLPGGVV II I I I I I I I I II I I I I l I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m639-l NSAFDLNGDGFGDSAYRPNGIIDQIIWRAPVSRLLMNSPAISIVKWAQAQFPAVLPGGVV 250 260 270 280 290 300 310 320 330 340 g639-l .pep DSKPLMKPYAPKIQTRYQAMKDELLKEAETRQSERGRAENGΞLNX I I I I I I I I I I II I I I I I I I I I I I I I I I : I I I I I I llllllllll m639-l DSKPLMKPYAPKIQTRYQAMKDELLKEVETRQSEWGRAENGΞLNX 310 320 330 340 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2049>: a639-l . seq 1 ATGAGCCTGC CCGCAATGGA TGCCGGTATT TATCTCGAAG AAACTGCCCC 51 GCGCGCCCTG ATTGAACACA ATAATATTTT GGATAATTCG GTCGGCGTCT 101 ATCTGCATGG TTCTGCCGAT GCGATGGTGC GGGAGAATAA AATCGTCGGC 151 GACGCGACTT TGCGCGTGAA CGAGCGCGGC AATGGCGTTA CCGTTTGGAA 201 CGCGCCCGGC GCGCAGGTCG TCGGCAACGA TATTTCCAAA GGGCGGGACG 251 GCATTTTTTC CAATACCAGC ACGCACAACA CCTATAAAAA CAACCGCTTC 301 AGCGATTTGC GTTTCGCCGT CCACTATATG TACACCAACG ACAGCGAAAT 351 CAGCGGCAAT ATTTCCGTGG GCAACAATAT GGGCTATGTG CTGATGTTTT 401 CCGAGCGGCT CAAAGTGTTT GACAATATCG CCGTCGGCAG CCGCGACCAA 451 GGCATCATGC TCAACTATGT CAACTATTCC GATATTCACG ACAACATTAT 501 CAACAAAGCG GGCAAGTGCG TTTTTGCCTA CAATGCCAAC TACGATAAAC 551 TGTCCGCCAA TCATTTTGAA AACTGCCAAA TCGGCATACA CTTTACCGCC 601 GCCATCGAAG GCACGTCCCT GCACGACAAT TCCTTTATCA ACAACGAAAG 651 CCAGGTCAAA TACGTCAGCA CGCGCTTTCT CGACTGGAGC GAGGGCGGAC 701 ACGGCAACTA TTGGAGCGAC AACAGCGCGT TCGATTTGAA CGGCGACGGC 751 TTCGGAGACA GCGCGTACCG TCCCAACGGC ATCATCGACC AAATCATCTG 801 GCGCGCACCC GTATCGCGCC TCTTGATGAA CAGTCCCGCA ATCAGCATCG 851 TCAAATGGGC GCAGGCGCAA TTTCCCGCCG TTTTGCCTGG CGGCGTGGTG 901 GACAGCAAAC CGCTGATGAA GCCTTATGCC CCCAAAATTC AAACCCGTTA 951 TCAGGCGATG AAGGACGGGC TGCTCAAAAA AGTCGAAACG CGGCAGTTGG 1001 AATGGGGCAG GGCGGAAAAC GGTTCTTTGA ACTAG This corresponds to the amino acid sequence <SEQ ID 2050; ORF 639-1. a>: a639-l.pep 1 MSLPAMDAGI YLEETAPRAL IEHNNILDNS VGVYLHGSAD AMVRENKIVG 51 DATLRVNERG NGVTVWNAPG AQVVGNDISK GRDGIFSNTS THNTYKNNRF 101 SDLRFAVHYM YTNDSEISGN ISVGNNMGYV LMFSERLKVF DNIAVGSRDQ 151 GIMLNYVNYS DIHDNIINKA GKCVFAYNAN YDKLSANHFE NCQIGIHFTA 201 AIEGTSLHDN SFINNESQVK YVSTRFLDWS EGGHGNYWSD NSAFDLNGDG 251 FGDSAYRPNG IIDQIIWRAP VSRLLMNSPA ISIVKWAQAQ FPAVLPGGVV 301 DSKPLMKPYA PKIQTRYQAM KDGLLKKVET RQLEWGRAEN GSLN* a639-l/m639-l 98. i identity in 344 aa overlap 10 20 30 40 50 60 a639-1. pep MSLPAMDAGIYLEETAPRALIEHNNILDNSVGVYLHGSADAMVRENKIVGDATLRVNERG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I m639-l MSLPAMDAGIYLEETAPRALIEHNNILDNΞVGVYLHGSADAMVRENKIVGDATLRVNERG 10 20 30 40 50 60 70 80 90 100 110 120 a639-l.pep NGVTVWNAPGAQVVGNDISKGRDGIFΞNTSTHNTYKNNRFSDLRFAVHYMYTNDSEIΞGN I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m639-l NGVTVWNAPGAQVVGNDISKGRDGIFSNTSTHNTYKNNRFSDLRFAVHYMYTNDSEISGN 70 80 90 100 110 120 130 140 150 160 170 180 a639-l.pep ISVGNNMGYVLMFSERLKVFDNIAVGSRDQGIMLNYVNYSDIHDNIINKAGKCVFAYNAN I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m639-l ISVGNNMGYVLMFSERLKVFDNIAVGSRDQGIMLNYVNYSDIHDNIINKAGKCVFAYNAN 130 140 150 160 170 180 190 200 210 220 230 240 a639-l.pep YDKLSANHFENCQIGIHFTAAIEGTSLHDNSFINNESQVKYVSTRFLDWSEGGHGNYWSD MM I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m639-l YDKLFANHFENCQIGIHFTAAIEGTΞLHDNSFINNEΞQVKYVSTRFLDWSEGGHGNYWSD 190 200 210 220 230 240 250 260 270 280 290 300 a639-1. pep NSAFDLNGDGFGDSAYRPNGIIDQIIWRAPVSRLLMNSPAISIVKWAQAQFPAVLPGGVV I I I I I II I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m639-1 NSAFDLNGDGFGDSAYRPNGIIDQIIWRAPVSRLLMNSPAISIVKWAQAQFPAVLPGGVV 250 260 270 280 290 300 310 320 330 340 a639-l .pep DSKPLMKPYAPKIQTRYQAMKDGLLKKVETRQLEWGRAENGSLNX I I I II I I I I I I II I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I m639-1 DSKPLMKPYAPKIQTRYQAMKDELLKEVETRQSEWGRAENGΞLNX 310 320 330 340 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2051>: g640. seq 1 ATGATTCATA TAATATCAAT ATTAAAGAGT ATCGGTATAT CGGGGATAGC 51 TATGTCCTGT TTTTCAATCC GGCGTATGTC TGCGTTTCGG GCGCGGATAA 101 CGGCGTTTTT TACCGCCTTT GTCTTTTTGA CGGcggcACT GCCCGCTTAT 151 GcggAgcgTc tgcctGATTT TCTGgcgAAA ATacAgcctT CGGAAATTTT 201 TCCGGGTGCG GATCGTTACG GCAAGCCGGA aggcAAGCCT AtggtTGCCC 251 GCgtttACAA AGgcgATGAG CAGCTCGGTT TGGTTTATAT CACGACCGAT 301 GCGGTCAATA CGCGCGGTTA TTCGAGCAAA CCGATCGATA CGCTGATGGC 351 TTTGGCAAAC GACGGCACGA TAGCCGGGGC GAAACTGGTC GATCATCACG 401 AACCGATTAT GCTGATCGGT ATCCCGCAAT CGCGTGTCGA TAAGTTCATC 451 GACAAATATA TCGGTCTGAA TTTTATTAAA AATCCGCCGA CCCCGAGCGT 501 GGCGCCGGGC GACATCATCA GcggtGCGAC TgttaCACTG ATGGTGGTTA 551 ACGACAGCAT CCAGCGTTCG TACAAGGTCA TTGCCAACCA ATACCGTCTG 601 GGTTCGGACA AGGCCCTTCA GACGGCATCC GCTTCCGATG TTCGGGAAGC 651 CGCGCCTGCG TCAGAAACCC GTCCGCGCCG TATGGCAAAT CCCGACAAGC 701 AGGATATTTT GTCTTGGGAC GAACTTTTGA AACAAAAGGC CGTCGGCCAT 751 CTGCATATCA CGCTCGATCA AATCAACAAA CTGTTTGAGA AAGGCGGCAA 801 GGCCGGCGTG GCCGATCACG CCGAACAGGG CGATCCTGAC GATACCTTTA 851 TTGATTTGTA TGTTGCCTTG GTCAGCCAGC CTTCCATCGG TAAAAGCCTG 901 CTGGGTGAGG ACGGCTGGGC GCATCTGCAA AAACGGCTGA AACCCGGGCA 951 GCAGGCGGTT TTGGTTGCCG GAGAGGGCCG TTATTCTTGG AAAGGTTCGG 1001 GCTATGTGCG CGGCGGTATT TTCGACCGTA TCGAGATGAT TCAGGGGGAG 1051 AACAGCTTCC GTTTTACCGA TGCCCAACAC GAACGCGTCG TCGAGCTGTC 1101 TGCCGCCGAT GCGCCGCGTT TTAAAGAAGT TTCTTGGTTT ACCATCCCTG 1151 AAGGCGTAGC GTTTGACGGT GCGGAGCCGT GGCGGCTGTA A This corresponds to the amino acid sequence <SEQ ID 2052; ORF 640.ng>: g640.pep 1 MIHIISILKS IGISGIAMSC FSIRRMSAFR ARITAFFTAF VFLTAALPAY 51 AERLPDFLAK IQPSEIFPGA DRYGKPEGKP MVARVYKGDE QLGLVYITTD 101 AVΝTRGYSSK PIDTLMALAΝ DGTIAGAKLV DHHEPIMLIG IPQSRVDKFI 151 DKYIGLΝFIK ΝPPTPSVAPG DIISGATVTL MWΝDSIQRS YKVIAΝQYRL 201 GSDKALQTAS ASDVREAAPA SETRPRRMAΝ PDKQDILSWD ELLKQKAVGH 251 LHITLDQIΝK LFEKGGKAGV ADHAEQGDPD DTFIDLYVAL VSQPSIGKSL 301 LGEDGWAHLQ KRLKPGQQAV LVAGEGRYSW KGSGYVRGGI FDRIEMIQGE 351 ΝSFRFTDAQH ERWELSAAD APRFKEVSWF TIPEGVAFDG AEPWRL* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2053>: m640.seq (partial) 1 ATGATTCATA TAATATCAAT ATTAAAGAGT ATCGGTATAT CGGGGATAGT 51 CATGTCCTGT TTTTCAATCA AACGTATGTC CGCGTTTCGG GCGCGGATAA 101 CGGCGTTTTT TGCCGCCTTT GTCTTTTTGA CGGCGGCACT GCCCGCTTAT 151 GCGGAGCGTC TGCCTGATTT TCTGGCGAAA ATACAGCCTT CGGAAATTTT 201 TCCGGGTGCG GACCGTTACG GCAAGCCGGA AGGTAAGCCT ATGGTTGCCC 251 GCGTTTACAA AGGCGATGAG CAGTTGGGCT TGGTCTATAT CACGACCGAT 301 GCGGTCAATA CGCGCGGTTA TTCGAGCAAA CCGATTGATA CGCTGATGGT 351 GTTGGCAAAC GACGGCACGA TAGCCGGGGC GAAACTGGTC GACCATCACG 401 AACCGATTAT GCTGATCGGT ATCCCGCAT... This corresponds to the amino acid sequence <SEQ ID 2054; ORF 640>: m640.pep (partial) 1 MIHIISILKS IGISGIVMSC FSIKRMSAFR ARITAFFAAF VFLTAALPAY 51 AERLPDFLAK IQPSEIFPGA DRYGKPEGKP MVARVYKGDE QLGLVYITTD 101 AVΝTRGYSSK PIDTLMVLAΝ DGTIAGAKLV DHHEPIMLIG IPH... m640/g640 96.5% identity in 143 aa overlap 10 20 30 40 50 60 m640.pep MIHIISILKSIGISGIVMSCFSIKRMSAFRARITAFFAAFVFLTAALPAYAERLPDFLAK I I I I I II II II II II I : II II II : I I II I I II II II I : I I II II II I I II II II II II M g640 MIHIISILKSIGISGIAMSCFSIRRMSAFRARITAFFTAFVFLTAALPAYAERLPDFLAK 10 20 30 40 50 60 70 80 90 100 110 120 m640. pep IQPSEIFPGADRYGKPEGKPMVARVYKGDEQLGLVYITTDAVNTRGYSSKPIDTLMVLAN II I I II II I I II I II II I II I I II II II I I II II II I I I II II II II II I II II I I : II I g640 IQPSEIFPGADRYGKPEGKPMVARVYKGDEQLGLVYITTDAVNTRGYSSKPIDTLMALAN 70 80 90 100 110 120 130 140 m640.pep DGTIAGAKLVDHHEPIMLIGIPH II II II I I II II I I II II II II : g640 DGTIAGAKLVDHHEPIMLIGIPQSRVDKFIDKYIGLNFIKNPPTPSVAPGDIISGATVTL 130 140 150 160 170 180 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2055>: a640.seq (partial) 1 ATGATTCATA TAATATCAAT ATTAAAGAGT ATCGGTATAT CGGGGATAGT 51 CATGTCCTGT TTTTCAATCA AACGTATGTC CGCGTTTCGG GCGCGGATAA 101 CGGCGTTTTT TGCCGCCTTT GTCTTTTTGA CGGCGGCACT GCCCGCTTAT 151 GCGGAGCGTC TGCCTGATTT TCTGGCGAAA ATACAGCCTT CGGAAATTGT 201 TCCGGGTGCG GACCGTTACA GCAAGCCGGA AGGTAAGCCT ATGGTTGCCC 251 GCGTTTACAA AGGCGATGAG CAGTTGGGCT TGGTCTATAT CACGACCGAT 301 GCGGTCAATA CGCGCGGTTA TTCGAGCAAA CCGATTGATA CGCTGATGGC 351 GTTGGCTAAA GACGGTACGA TAGCCGGAGC GAAATTGGTT GATCACCATG 401 AGTCGATTAT GCTGATCGGT ATCCCGCAT... This corresponds to the amino acid sequence <SEQ ID 2056; ORF 640. a>: a640.pep (partial) Length: 143 1 MIHIISILKS IGISGIVMSC FSIKRMSAFR ARITAFFAAF VFLTAALPAY 51 AERLPDFLAK IQPSEIVPGA DRYSKPEGKP MVARVYKGDE QLGLVYITTD 101 AVΝTRGYSSK PIDTLMALAK DGTIAGAKLV DHHESIMLIG IPH... m640/a640 96.5% identity in 143 aa overlap 10 20 30 40 50 60 m640.pep MIHIISILKSIGISGIVMSCFSIKRMSAFRARITAFFAAFVFLTAALPAYAERLPDFLAK II I II II I II II II I I II II II II II II II I II I I II II II II I II II II I II II II I II a640 MIHIISILKSIGISGIVMSCFSIKRMSAFRARITAFFAAFVFLTAALPAYAERLPDFLAK 10 20 30 40 50 60 70 80 90 100 110 120 m640.pep IQPSEIFPGADRYGKPEGKPMVARVYKGDEQLGLVYITTDAVΝTRGYSSKPIDTLMVLAΝ llllll I II II I : I II I I II II I I I II II I II I I II I II II II I II II II II II I : I I : a640 IQPSEIVPGADRYSKPEGKPMVARVYKGDEQLGLVYITTDAVΝTRGYSSKPIDTLMALAK 70 80 90 100 110 120 130 140 m640.pep DGTIAGAKLVDHHEPIMLIGIPH II II I II I II II II I II I II II a640 DGTIAGAKLVDHHESIMLIGIPH 130 140 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 2057>: g642. seq 1 ATGCGGTATC CGCCGCAATC GGCGGTTTTG CAGAATGCCG CGCGTTGCCT 51 TTTGCGCCGC CCGAAATCTG CCTGCCGCCG TATTTGCCCG CTATCCGCAA 101 TATCGGCAGT CCAATATATC TTTGCGGATG TCGTTCAGCA GGAAGGCTGT 151 GGTGTCTTCG TGTTCCTCCT GTACGAAGAC AAAAAGTCGG GCGATGATTT 201 TGCCGATGAA GACTTTTTGC AGGGCGCAGG CGTCGGTCAG GGTGTGTTCC 251 TGCAGGAAGC TGCGGATGTC TTCGGGCAAA GCGTAgtCgc gGGCAACGGC 301 GGcaaagcgG ACatcggtTT Gcacggcgtc gagCAGGGtt tggtTTTTGT 351 CCAACTTAAT GCCTGCTTCT TTTTCTTCGG CGGTGGCGCG GACGAACTGG 401 TCGTAAATTT CGGCATAAAG CATATCGTTC GGGCCTTCAA AAATCGTGAA 451 GGGGCGGATA TCGATGGCGA TATTGCCGGC TGGGTGTCCG CGTTCAAAAC 501 CCTTCGCGCC CAAGAGTTTT TGCAACATTT GCGCGGCGgc gTAAGTGTAT 551 TCCGTGGCGa ggGTTTTGAc gatgTTCGCC TCCATCAATT GATGGGCGAc 601 ggGCGcgacg ggCGAAACGG AATGGCAGAC GTAGCGGTAA AGGATTTCGG 651 AAACCTGATG GCGGCGTTGG ATTTCGCGGC GTTCGTAATC GACGAATCTG 701 ATATCGTTGC GGACATATCG GTTCAGGTTG TCAAGGATGT ATTCCATAAT 751 GCCGTGCGTC ATGCCGATCA GTTGCAGGCG GCTGCGGATA AAGATGTTTT 801 GGAACGCGCG CAAACCGGCA GCGTCGCCCC GGGAGAGTTT CATCACGGCG 851 GTTGCAGGCA TTTCGGCATC GATGCGGTTG ACGGCGTAAC GGACGGCGCG 901 CAGGCCTTCG GATGCGAGGG TTTCGCAGCG GATGTATGTT TTGGGGACGA 951 GCAGCAGGTC GATGactttg gcgagtttgC Cgtttttgcg ctctttggcg 1001 gcaacgaggA GGAAGTCGCT TTGCGAATTG CCCTGCCAGT ATTTCGCGGC 1051 GttgACGTAA ATGGTTtgtt cgtcggtata ttcgtagcag gactgcaTTT 1101 CGCGTGCAAt cgCcgcgccg gaggtTtcgg gttcggtaAc gcccaaacgg 1151 cggctttcgc ctTTGAAAAT CATGTCCAAA CCTTGTGCGA CTTGCgcttc 1201 gccgccgaac tCTTGCAGAG GCTGCAACAC CAGCGCGCCT TCGATGCCGG 1251 TACGCAGCGT AACGGGCACG CCGTAATGCC CCGCAATCCT TAG This corresponds to the amino acid sequence <SEQ ID 2058; ORF 642.ng>: g642.pep 1 MRYPPQSAVL QNAARCLLRR PKSACRRICP LSAISAVQYI FADWQQEGC 51 GVFVFLLYED KKSGDDFADE DFLQGAGVGQ GVFLQEAADV FGQSWAGNG 101 GKADIGLHGV EQGLVFVQLN ACFFFFGGGA DELWNFGIK HIVRAFKNRE 151 GADIDGDIAG WVSAFKTLRA QEFLQHLRGG VSVFRGEGFD DVRLHQLMGD 201 GRDGRNGMAD VAVKDFGNLM AALDFAAFVI DESDIVADIS VQWKDVFHN 251 AVRHADQLQA AADKDVLERA QTGSVAPGEF HHGGCRHFGI DAVDGVTDGA 301 QAFGCEGFAA DVCFGDEQQV DDFGEFAVFA LFGGNEEEVA LRIALPVFRG 351 VDVNGLFVGI FVAGLHFACN RRAGGFGFGN AQTAAFAFEN HVQTLCDLRF 401 AAELLQRLQH QRAFDAGTQR NGHAVMPRNP * The following partial DNA sequence was identified in N meningitidis <SEQ ID 2059>: m642.seq (partial) 1 GCCTGCCGCC GTATTTGCCC GCTACCCGCA ATATCGGCAG TCCAATATAT 51 CTTTGCGGAT GTCGTTCAGC AGGAAGGCTG CGGTGTCTTC GTGTTTCGCC 101 TGTACGAAGA CAAAGAGTCG GGCGATGATT TTGCCGATAA AGACTTTTTG 151 CAGGGCGCAG GCATCGGTCA GGGTGTGTTC CTGCAGGAAG CTGCGGATGT 201 CTTCAGGCAA AGTGTAGTCG CGGGCGACGG CGGCAAAGCG GGCATCGGTT 251 TGCAGGCGGT CGAGCAGGGT TTGGTTTTTG TCCAACTTCA TGCCTGCTTC 301 TTTTTCTTCG GCGGTGGCGC GGACAAACTG GTCGTAAATT TCGGCATAAA 351 GCATATCGTT CGGGCCTTCA AAAATCGTGA AGGGGCGGAT GTCGATAGCG 401 ATATTGCCGG CGGTGTGTCC GCGTTCAAAA CCCTTCGCAC CCAAGAGTTT 451 TTGCAACATT TGCGCGGCGG CGTAAGTGTA TTCCGTGGCG AGGGTTTTGA 501 CGATGTTCGC CTCCATCAGC TGATGGGCGA CGGGGGCAAC AGGCGAAACG 551 GAATGGCAGA CGTAGCGGTA AAGAATCTCG GAAACCTGAT GGCGGCGCCG 601 GATTTCGCGC CGTTCGTAAT CGACGAATTT GATGTCGTTG CGGACGTATC 651 GTTCCAGATT TTCAAGGATG TATTCCATAA TGCCGTGCGT CATGCCGATC 701 AGTTGCAGGC GGCTGCGGAT AAAGATGTTT TGGAACGCGC GCAAACCGGC 751 AGCGTCGCTC TGGGAGAGTT TCATCACGGC GGTTGCAGGC ATTTCGGCAT 801 CGATGCGGTT GACGGCGTAA CGGACGGCGC GCAAGCCTTC GGATGCGAGG 851 GTTTCGCAGC GGATGTATGT TTTGGGGACG AGCAGCAGGT CGATGACTTT 901 GGCGAGTTTG CCGTTTTTGC GCTCTTTGGC GGCAACGAGG AGGAAGTCGC 951 TTTGCGAGTT GCCCTGCCAG TATTTCGCGG CGTTGACGTA AATGGTTTGT 1001 CCGTCGATAT ATTCGTAGTA GGACTGCATT TCGCGTGCAA TCGCCGCGCC 1051 GGAGGTTTCG GGTTCGGTAA CACCCAAACC GCCGCCCTCG CCTTTGAAAA 1101 TCATCTCCAA ACCTTGCGCG ACTTGCGCTT CATCGCCGAA CTCTTGCAGT 1151 GGCTGCAACA CCAGCGCGCC TTCGATGCCG GTACGCAGCG TAACGGGCAC 1201 GCCGTAATGC CCCGCAATCC G This corresponds to the amino acid sequence <SEQ ID 2060; ORF 642>: m642.pep (partial) 1 ACRRICPLPA ISAVQYIFAD WQQEGCGVF VFRLYEDKES GDDFADKDFL 51 QGAGIGQGVF LQEAADVFRQ SWAGDGGKA GIGLQAVEQG LVFVQLHACF 101 FFFGGGADKL WΝFGIKHIV RAFKΝREGAD VDSDIAGGVS AFKTLRTQEF 151 LQHLRGGVSV FRGEGFDDVR LHQLMGDGGΝ RRΝGMADVAV KΝLGΝLMAAP 201 DFAAFVIDEF DWADVSFQI FKDVFHΝAVR HADQLQAAAD KDVLERAQTG 251 SVALGEFHHG GCRHFGIDAV DGVTDGAQAF GCEGFAADVC FGDEQQVDDF 301 GEFAVFALFG GΝEEEVALRV ALPVFRGVDV ΝGLSVDIFW GLHFACΝRRA 351 GGFGFGΝTQ AALAFEΝHLQ TLRDLRFIAE LLQWLQHQRA FDAGTQRΝGH 401 AVMPRΝP m642/g642 90.4% identity in 407 aa overlap 10 20 30 m642.pep ACRRICPLPAISAVQYIFADWQQEGCGVFVFRLYED II II II I I I I I II II I II II I I I II II II II llll g642 MRYPPQSAVLQNAARCLLRRPKSACRRICPLSAISAVQYIFADWQQEGCGVFVFLLYED 10 20 30 40 50 60 40 50 60 70 80 90 m642.pep KESGDDFADKDFLQGAGIGQGVFLQEAADVFRQSVVAGDGGKAGIGLQAVEQGLVFVQLH I M I I I II I : II II II I : II II I I I II I II I II II II : II II II I : : II II II I II M g642 KKSGDDFADEDFLQGAGVGQGVFLQEAADVFGQSWAGNGGKADIGLHGVEQGLVFVQLN 70 80 90 100 110 120 100 110 120 130 140 150 m642. pep ACFFFFGGGADKLWNFGIKHIVRAFKNREGADVDSDIAGGVSAFKTLRTQEFLQHLRGG I II II II II II : II II | I II II I I I II I II II I : I : I II I II I II II M II I II I I I II g642 ACFFFFGGGADELWNFGIKHIVRAFKNREGADIDGDIAGWVSAFKTLRAQEFLQHLRGG 130 140 150 160 170 180 160 170 180 190 200 210 m642. pep VSVFRGEGFDDVRLHQLMGDGGNRRNGMADVAVKNLGNLMAAPDFAAFVIDEFDVVADVS II II I I II I II II II II II I I : II II I I I II I :: I II II I lllllllll hill: g642 VSVFRGEGFDDVRLHQLMGDGRDGRNGMADVAVKDFGNLMAALDFAAFVIDESDIVADIS 190 200 210 220 230 240 220 230 240 250 260 270 m642.pep FQIFKDVFHNAVRHADQLQAAADKDVLERAQTGSVALGEFHHGGCRHFGIDAVDGVTDGA M II II II II I I II II II I II I I II I II II I II I II I II II II I I II II II I II II I g642 VQWKDVFHNAVRHADQLQAAADKDVLERAQTGSVAPGEFHHGGCRHFGIDAVDGVTDGA 250 260 270 280 290 300 280 290 300 310 320 330 m642. pep QAFGCEGFAADVCFGDEQQVDDFGEFAVFALFGGNEEEVALRVALPVFRGVDVNGLSVDI II I I I II I II II II I II II I II II II II II I II I II I II II I : II II II II II I I I I I g642 QAFGCEGFAADVCFGDEQQVDDFGEFAVFALFGGNEEEVALRIALPVFRGVDVNGLFVGI 310 320 330 340 350 360 340 350 360 370 380 390 m642.pep FVVGLHFACNRRAGGFGFGNTQTAALAFENHLQTLRDLRFIAELLQWLQHQRAFDAGTQR I I : I I I II II I I I I II II II : I II I : I II I I : II I II I I II I II lllllllllllll g642 FVAGLHFACNRRAGGFGFGNAQTAAFAFENHVQTLCDLRFAAELLQRLQHQRAFDAGTQP 370 380 390 400 410 420 400 m642.pep NGHAVMPRNP I I I I I I I I I I g642 NGHAVMPRNPX 430 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2061>: a642.seq (partial) 1 GCCTGCCGCC GTATTTGCCC GCTATCCGCA ATATCGGCAG TCCAATATGT 51 CTTTGCGGAT GTCGTTCAGC AGGAAGGCTG CGGTGTCTTC GTGTTCCGCC 101 TGTACGAAGA CAAAGAGTCG GGCGATGATT TTGCCGATAA AGACTTTTTG 151 CAGGGCGCAG GCATCGGTCA GGGTGTGTTC CTGCAGGAAG CTGCGGATGT 201 CTTCGGGCAA AGTGTAGTCG CGGGCGACGG CGGCAAAGCG GGCATCGGTT 251 TGCAGGCGGT CGAGCAGGGT TTGGTTTTTG TCCAACTTCA TGCCTGCTTC 301 TTTTTCTTCG GCGGTGGCGC GGACAAACTG GTCGTAAATT TCGGCATAAA 351 GCATATCGTT CGGGCCTTCA AAAATCGTGA AGGGGCGGAT GTCGATAGCG 401 ATATTGCCGG CGGTGTGTCC GCGTTCAAAA CCCTTCGCGC CCAAGAGTTT 451 TTGCAACATT TGCGCGGCGG CGTAAGTGTA TTCCGTGGCG AGGGTTTTGA 501 CGATGTTCGC CTCCATCAGT TGATGGGCGA CGGGTGCAAC GGGCGAAACG 551 GAATGGCAGA CGTAGCGGTA AAGAATCTCG GAAACCTGAT GGCGGCGCCG 601 GATTTCGCGG CGTTCGTAAT CGACGAATCT GATGTCGTTG CGGACGTATC 651 GTTCCAGGTT TTCAAGGGTG TATTCCATAA TGCCGTGCGT CATGCCGATC 701 AGTTGCAGGC GGCTGCGGAT AAAGATGTTT TGGAACGCGC GCAAACCGGC 751 AGCGTCGCTC TGGGAGAGTT TCATCACGGC GGTTGCAGGC ATTTCGGCAT 801 CGATGCGGTT GACGGCGTAA CGGACGGCGC GCAAGCCTTC GGATGCGAGG 851 GTTTCGCAGC GGATGTATGT TTTGGGGACG AGCAGCAGGT CGATGACTTT 901 GGCGAGTTTG CCGTTTTTGC GCTCTTTGGC GGCAACGAGG AGGAAGTCGC 951 TTTGCGAGTT GCCCTGCCAG TATTTCGCGG CGTTGACGTA AATGGTTTGT 1001 CCGTCGGTAT ATTCGTAGTA AGACTGCATT TCTCGGGCAA TCGCCGCGCC 1051 GGAGGTTTCG GGTTCGGTAA CGCCTAAACC GCCGCCCTCG CCTTTGAAAA 1101 CCATGTCCAA ACCCTGTGCG ATTTGCGCTT CATCGCCGAA CTCTTGCAGT 1151 GGCTGCAACA CCAGCGCGCC TTCGATGCCG GTACGCAGCG TAACGGGCAC 1201 GCCGTAATGC CCCGCAATCC G This corresponds to the amino acid sequence <SEQ ID 2062; ORF 642. a>: a642.pep Length: 407 1 ACRRICPLSA ISAVQYVFAD WQQEGCGVF VFRLYEDKES GDDFADKDFL 51 QGAGIGQGVF LQEAADVFGQ SVVAGDGGKA GIGLQAVEQG LVFVQLHACF 101 FFFGGGADKL WNFGIKHIV RAFKNREGAD VDSDIAGGVS AFKTLRAQEF 151 LQHLRGGVSV FRGEGFDDVR LHQLMGDGCN GRNGMADVAV KNLGNLMAAP 201 DFAAFVIDES DWADVSFQV FKGVFHNAVR HADQLQAAAD KDVLERAQTG 251 SVALGEFHHG GCRHFGIDAV DGVTDGAQAF GCEGFAADVC FGDEQQVDDF 301 GEFAVFALFG GNEEEVALRV ALPVFRGVDV NGLSVGIFW RLHFSGNRRA 351 GGFGFGNA*T AALAFENHVQ TLCDLRFIAE LLQWLQHQRA FDAGTQRNGH 401 AVMPRNP m642/a642 95.8% identity in 407 aa overlap 10 20 30 40 50 60 m642 . pep ACRRICPLPAISAVQYIFADWQQEGCGVFVFRLYEDKESGDDFADKDFLQGAGIGQGVF I I I I I M I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a642 ACRRICPLSAISAVQYVFADVVQQEGCGVFVFRLYEDKESGDDFADKDFLQGAGIGQGVF 10 20 30 40 50 60 70 80 90 100 110 120 m642. pep LQEAADVFRQSVVAGDGGKAGIGLQAVEQGLVFVQLHACFFFFGGGADKLWNFGIKHIV llllllll II II II I II II II II II II II II I II II II II II II II II I II II II II II a642 LQEAADVFGQSWAGDGGKAGIGLQAVEQGLVFVQLHACFFFFGGGADKLVVNFGIKHIV 70 80 90 100 110 120 130 140 150 160 170 180 m642.pep RAFKNREGADVDSDIAGGVSAFKTLRTQEFLQHLRGGVSVFRGEGFDDVRLHQLMGDGGN I I II I I II II II I II II II I I I II II : I II II I II II I II II II II II II II II II II I a642 RAFKNREGADVDSDIAGGVSAFKTLRAQEFLQHLRGGVSVFRGEGFDDVRLHQLMGDGCN 130 140 150 160 170 180 190 200 210 220 230 240 m642. pep RRNGMADVAVKNLGNLMAAPDFAAFVIDEFDWADVSFQIFKDVFHNAVRHADQLQAAAD I II I I II II I II II II II I II II I II II II II I II I I : II II II II II II II I II II a642 GRNGMADVAVKNLGNLMAAPDFAAFVIDESDWADVSFQVFKGVFHNAVRHADQLQAAAD 190 200 210 220 230 240 250 260 270 280 290 300 m642.pep KDVLERAQTGSVALGEFHHGGCRHFGIDAVDGVTDGAQAFGCEGFAADVCFGDEQQVDDF MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a642 KDVLERAQTGSVALGEFHHGGCRHFGIDAVDGVTDGAQAFGCEGFAADVCFGDEQQVDDF 250 260 270 280 290 300 310 320 330 340 350 360 m642. pep GEFAVFALFGGNEEEVALRVALPVFRGVDVNGLSVDIFWGLHFACNRRAGGFGFGNTQT II I II II II II I I II I II II I I I I I II II II II II MM M II I I II II II I : I a642 GEFAVFALFGGNEEEVALRVALPVFRGVDVNGLSVGIFWRLHFSGNRRAGGFGFGNAXT 310 320 330 340 350 360 370 380 390 400 m642. pep AALAFENHLQTLRDLRFIAELLQWLQHQRAFDAGTQRNGHAVMPRNP II II II II : II I I II I I II I I I II I II I II I I I II I I I I I I I I I I I a642 AALAFENHVQTLCDLRFIAELLQWLQHQRAFDAGTQRNGHAVMPRNP 370 380 390 400 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2063>: g643. seq 1 ATGGTGTTGC CTTTGATGTT GTTGGCGACA ATCAGgTcgg CTACGCTGAc 51 gttgtancGt TTGGcaATGt tGaaCAgggt gtcgccTTCT ACAACGCGGT 101 GGATGCTGGC ATGGagcGGG GAGGTTTCGG CTTCGCCGTC GGCAGCTTTG 151 GCTACGCGCG TTTCCAAACG TGCCCGGCGT TtgCCGTCGG CGGCAACGGT 201 ATGTTGCGGA GATGAGGAAA TGTTGTGTTC GGCAACTGTG TCAGGCGTGC 251 CGATGACGGC GGagaTGGTT TCTTCAGCCT GCCGGCGCag gTTGTTTCGG 301 GCAACAAGCT GCATGAGTTC GTCTGCCGCC TGCATGTCGT TTGGGGGGAt 351 GACCTGCGCg aGTGtTGCGG TTTGGGTTTC agacgGCATG GCAGTCTGTT 401 TTTcggTTTG a This corresponds to the amino acid sequence <SEQ ID 2064; ORF 643>: g643.pep 1 MVLPLMLLAT IRSATLTLXR LAMLΝRVSPS TTRWMLAWSG EVSASPSAAL 51 ATRVSKRARR LPSAATVCCG DEEMLCSATV SGVPMTAEMV SSACRRRLFR 101 ATSCMSSSAA CMSFGGMTCA SVAVWVSDGM AVCFSV* The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 2065>: m643. seq 1 ATGGTGTTGC CTTTGATGTT GTTGGCGACA ATCAGGTCGG CTACACTGAC 51 GTTGTAGCGT TTGGCAATGT TGAACAGGGT GTCGCCTTCT ACAACGCGGT 101 GGATGCTGGC ATGGAGCGGG GAGATTTCGG CTTCGCCGTC GGCAGCTTTG 151 GCTACGCGCG TTTCCAAACG TACCCGGCGT TTGCCGTCGG CGGCAGCGGT 201 ATGTTGCGGA GATGCGGAAA TTTTGTGTTC GGCAACTGTG TCAGGCGTGC 251 CGATGACGGC GGAGATGGTT TCTTCAGCCT GTCGGCGCAG GTTGTTTCGG 301 GCAACAAGCT GCATGAGTTC GTCTGCCGCC TGCATGTCGT TTTGGGGGAT 351 GATCTGCGCG AGTGTTGCGG TTTGGGTTTC AGACGGCATG GCGGTCTGTT 401 TTTCGGTTTG A This corresponds to the amino acid sequence <SEQ ID 2066; ORF 643>: m643.pep 1 MVLPLMLLAT IRSATLTL*R LAMLΝRVSPS TTRWMLAWSG EISASPSAAL 51 ATRVSKRTRR LPSAAAVCCG DAEILCSATV SGVPMTAEMV SSACRRRLFR 101 ATSCMSSSAA CMSFWGMICA SVAVWVSDGM AVCFSV* Computer analysis of the amino acid sequences gave the following results: Homology with a predicted ORF from N meningitidis menA with menB ORF 643 shows 94.9% identity over a 136 aa overlap with a predicted ORF (ORF643.a) from N gonorrhoeae: m643/g643 10 20 30 40 50 60 m643.pep MVLPLMLLATIRSATLTLXRLAMLΝRVSPSTTRWMLAWSGEISASPSAALATRVSKRTRR MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g6 3 MVLPLMLLATIRSATLTLXRLAMLΝRVSPSTTRWMLAWSGEVSASPSAALATRVSKRARR 10 20 30 40 50 60 70 80 90 100 110 120 m643. pep LPSAAAVCCGDAEILCSATVSGVPMTAEMVSSACRRRLFRATSCMSSSAACMSFWGMICA II I II : II II I I : II II II II I II II I I II I I I I II I I II II II I II II II II II II g643 LPSAATVCCGDEEMLCSATVSGVPMTAEMVSSACRRRLFRATSCMSSSAACMSFGGMTCA 70 80 90 100 110 120 130 m6 3. pep SVAVWVSDGMAVCFSVX II I I II I I II I II II II g643 SVAVWVSDGMAVCFSVX 130 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2067>: a643. seq 1 ATGGTGTTGC CTTTGATGTT GTTGGCGACA ATCAGGTCGG CTACACTGAC 51 GTTGTAGCGT TTGGCAATGT TGAACAGGGT GTCGCCTTCT ACAACGCGGT 101 GGATGCTGGC ATGGAGCGGG GAGATTTCGG CTTCGCCGTC GGCAGCTTTG 151 GCTACGCGCG TTTCCAAACG TACCCGGCGT TTGCCGTCGG CGGCAACGGT 201 ATGTTGCGGA GATGAGGAAA TGTTGTGTTC GGCAACTGTG TCAGGCGTGC 251 CGATGACGGC AGAGATGGTT TCTTCAGCCT GTCGGCGCAG GTTGTTTCGG 301 GCAACAAGCT GCATGAGTTC GTCTGCCGCC TGCATGTCGT TTTGGGGGAC 351 GATCTGCGCG AGTGTTGCGG TTTGGGTTTC AGACGGCATG GCGGTCTGTT 401 TTTCGGTTTG A This corresponds to the amino acid sequence <SEQ ID 2068; ORF 643. a>: a643.pep 1 MVLPLMLLAT IRSATLTL*R LAMLNRVSPS TTRWMLAWSG EISASPSAAL 51 ATRVSKRTRR LPSAATVCCG DEEMLCSATV SGVPMTAEMV SSACRRRLFR 101 ATSCMSSSAA CMSFWGTICA SVAVWVSDGM AVCFSV* m643/a643 97.1% identity in 136 aa overlap 10 20 30 40 50 60 m643. pep MVLPLMLLATIRSATLTLXRLAMLNRVSPSTTRWMLAWSGEISASPSAALATRVSKRTRR MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a643 MVLPLMLLATIRSATLTLXRLAMLNRVSPSTTRWMLAWSGEISASPSAALATRVSKRTRR 10 20 30 40 50 60 70 80 90 100 110 120 m643. pep LPSAAAVCCGDAEILCSATVSGVPMTAEMVSSACRRRLFRATSCMSSSAACMSFWGMICA I I I I I i I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a643 LPSAATVCCGDEEMLCSATVSGVPMTAEMVSSACRRRLFRATSCMSSSAACMSFWGTICA 70 80 90 100 110 120 130 m6 3. pep SVAVWVSDGMAVCFSVX II II I I I II II II II II a643 SVAVWVSDGMAVCFSVX 130 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2069>: g644. seq 1 ATGCCGTCTG AAAGGccgGC GGATTGTTGC CCGGTGCACT TTGTGGTAAA 51 GTTTAGAAAA TTAACTCTAA ACTGTGGCAG GCGGTTTGAC CGGCCGCCGA 101 TTAATGGGAA CCGACAGAGG AAGCCGATGA TACACACCGA ACCGAGCGCG 151 CAGCCGTCAA CCATGGACAC GGCTGCTTTT TTAAagcaca tcgaatCCGC 201 ATTcCCCCGC ATTTTTTCAG ACGGCATCGA CCTGATGCGA TACCTGCCCG 251 AAGACAAATG GCTTGCCTTG AAGCAGGCGG GTTTGCTGTT GCCCTTCCTC 301 GACAAAAAAC ACGGCGGGCG CAAGGGCAGT CAGTTTGAAA TCCAAGAAGT 351 CCTAAGGATT GCGGGGCATT ACGGCGTGCC CGTTACGCTG CGTACCGGCA 401 TCGAAGGCGC GCTGGTGTTG CAGCCTCTGC AAGagttcgg cggcgaagcG 451 CAAGTCGCAC AAGGTTTGGA CATGATTTTC AAaggcgaaa gccgccgttt 501 gggcgTtacc gaacccgaAa cctccggcgc gGcgaTTGCA CGCGAAAtgc 551 agtcctgcta cgaatatacc gacgaacaAA CCATTTACGT caaCGCCGCG 601 AAATACTGGC AGGGCAATTC GCAAAGCGAC TTCCTcctcg ttgccgccaa 651 agagcgcaaa aacGGcaaac tcgccaaagt CATCGACCTG CTGCTCGTCC 701 CCAAAACATA CATCCGCTGC GAAACCCTCG CATCCGAAGG CCTGCGCGCC 751 GTCCGTTACG CCGTCAACCG CATCGATGCC GAAATGCCTG CAACCGCCGT 801 GATGAAACTC TCCCGGGGCG ACGCTGCCGG TTTGCGCGCG TTCCAAAACA 851 TCTTTATCCG CAGCCGCCTG CAACTGATCG GCATGACGCA CGGCATTATG 901 GAATACATCC TTGACAACCT GAACCGATAT GTCCGCAACG ATATCAGATT 951 CGTCGATTAC GAACGCCGCG AAATCCAACG CCGCCATCAG GTTTCCGAAA 1001 TCCTTTACCG CTACGTCTGC CATTCCGTTT CGcccgtcgC GCccgTCGCC 1051 CATCAATTGA TGGAGGCGAA catcgTCAAA ACcctCGCCA CGGAATACAC 1101 TTAcgcCGCC GCGCAAATGT TGCAAAAACT CTTGGGCGCG AAGGGTTTTG 1151 AACGCGGACA CCCAGCCGGC AATATCGCCA TCGATATCCG CCCCTTCACG 1201 ATTTTTGAAG GCCCGAACGA TATGCTTTAT GCCGAAATTT ACGACCAGTT 1251 CGTCCGCGCC ACCGCCGAAG AAAAAGAAGC AGGCATTAAG TTGGACAAAA 1301 accaaaCCCT Gctcgacgcc gtgCAAaccg atGTCcgctt tgCCGCCGTT 1351 GCCcgcGacT ACGCTTTGCC CGAAGACATC CGCAGCTTCC TGCAGGAACA 1401 CACCCTGACC GACGCCTGCG CCCTGCAAAA AGTCTTCATC GGCAAAATCA 1451 TCGCCCGACT TTTTGTCTTC GTACAGGAGG AACACGAAGA CACCACAGCC 1501 TTCCTGCTGA ACGACATCCG CAAAGATATA TTGGACTGCC GATATTGCGG 1551 ATAG This corresponds to the amino acid sequence <SEQ ID 2070; ORF 644.ng>: g64 .pep 1 MPSERPADCC PVHFWKFRK LTLNCGRRFD RPPINGNRQR KPMIHTEPSA 51 QPSTMDTAAF LKHIESAFPR IFSDGIDLMR YLPEDKWLAL KQAGLLLPFL 101 DKKHGGRKGS QFEIQEVLRI AGHYGVPVTL RTGIEGALVL QPLQEFGGEA 151 QVAQGLDMIF KGESRRLGVT EPETΞGAAIA REMQSCYEYT DEQTIYVNAA 201 KYWQGNSQSD FLLVAAKERK NGKLAKVIDL LLVPKTYIRC ETLASEGLRA 251 VRYAVNRIDA EMPATAVMKL SRGDAAGLRA FQNIFIRSRL QLIGMTHGIM 301 EYILDNLNRY VRNDIRFVDY ERREIQRRHQ VSEILYRYVC HSVSPVAPVA 351 HQLMEANIVK TLATEYTYAA AQMLQKLLGA KGFERGHPAG NIAIDIRPFT 401 IFEGPNDMLY AEIYDQFVRA TAEEKEAGIK LDKNQTLLDA VQTDVRFAAV 451 ARDYALPEDI RSFLQEHTLT DACALQKVFI GKIIARLFVF VQEEHEDTTA 501 FLLNDIRKDI LDCRYCG* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2071>: m644.seq 1 ATGCCGTCTG AAAGGTCGGC GGATTGTTGC CCGGCGCACT TTGTGGTAAA 51 GTTTAGAAAA TCAACTCTAA ACTGTGGCAG GCGGTTTGAC CGGCCGCCGA 101 TTAATGGGAA CCGACAGAGG AAGCCGATGA TACACACCGA ACCGAGCGCG 151 CAGCCGTCAA CTATGGACAC GGCTGCTTTT TTAAAGCACA TCGAATCCGC 201 ATTCCGCCGC ATTTTTTCAG ACGGTATCGA CCTGATGCGA TACCTGCCCG 251 AAGACAAATG GCTTGCCTTG AAGCAGGCGG GTTTGCTGTT GCCCTTCCTC 301 GACAAAAAAT ACGGCGGGCG CAAGGGCAGC CAGTTTGAAA TCCAAGAAGT 351 CcTGCGGATT GCGGGGCATT ACGGCGTGCC CGTTACGCTG CGTACCGGCA 401 TCGAAGGCGC GCTGGTGTTG CAGCCACTGC AAGAGTTCGG CGATGAAGCG 451 CAAGTCGCGC AAGGTTTGGA GATGATTTTC AAAGGCGAGG GCGGCGGTTT 501 GGGTGTTACC GAACCCGAAA CCTCCGGCGC GGCGATTGCA CGCGAAATGC 551 AGTCCTACTA CGAATATATC GACGGACAAA CCATTTACGT CAACGCCGCG 601 AAATACTGGC AGGGCAACTC GCAAAGCGAC TTCCTCCTCG TTGCCGCCAA 651 AGAGCGCAAA AACGGCAAAC TCGCCAAAGT CATCGACCTG CTGCTCGTCC 701 CCAAAACATA CATCCGCTGC GAAACCCTCG CATCCGAAGG CTTGCGCGCC 751 GTCCGTTACG CCGTCAACCG CATCGATGCC GAAATGCCTG CAACCGCCGT 801 GATGAAACTC TCCCAGAGCG ACGCTGCCGG TTTGCGCGCG TTCCAAAACA 851 TCTTTATCCG CAGCCGCCTG CAACTGATCG GCATGACGCA CGGCATTATG 901 GAATACATCC TTGAAAATCT GGAACGATAC GTCCGCAACG ACATCAAATT 951 CGTCGATTAC GAACGCCGCG AAATCCGGCG CCGCCATCAG GTTTCCGAGA 1001 TTCTTTACCG CTACGTCTGC CATTCCGTTT CGCCTGTTGC CCCCGTCGCC 1051 CATCAGCTGA TGGAGGCGAA CATCGTCAAA ACCCTCGCCA CGGAATACAC 1101 TTACGCCGCC GCGCAAATGT TGCAAAAACT CTTGGGTGCG AAGGGTTTTG 1151 AACGCGGACA CACCGCCGGC AATATCGCTA TCGACATCCG CCCCTTCACG 1201 ATTTTTGAAG GCCCGAACGA TATGCTTTAT GCCGAAATTT ACGACCAGTT 1251 TGTCCGCGCC ACCGCCGAAG AAAAAGAAGC AGGCATGAAG TTGGACAAAA 1301 ACCAAACCCT GCTCGACCGC CTGCAAACCG ATGCCCGCTT TGCCGCCGTC 1351 GCCCGCGACT ACACTTTGCC TGAAGACATC CGCAGCTTCC TGCAGGAACA 1401 CACCCTGACC GATGCCTGCG CCCTGCAAAA AGTCTTTATC GGCAAAATCA 1451 TCGCCCGACT CTTTGTCTTC GTACAGGCGA AACACGAAGA CACCGCAGCC 1501 TTCCTGCTGA ACGACATCCG CAAAGATATA TTGGACTGCC GATATTGCGG 1551 GTAG This corresponds to the amino acid sequence <SEQ ID 2072; ORF 644>: m644.pep 1 MPSERΞADCC PAHFWKFRK STLΝCGRRFD RPPIΝGΝRQR KPMIHTEPSA 51 QPSTMDTAAF LKHIESAFRR IFSDGIDLMR YLPEDKWLAL KQAGLLLPFL 101 DKKYGGRKGS QFEIQEVLRI AGHYGVPVTL RTGIEGALVL QPLQEFGDEA 151 QVAQGLEMIF KGEGGGLGVT EPETSGAAIA REMQSYYEYI DGQTIYVΝAA 201 KYWQGNSQSD FLLVAAKERK NGKLAKVIDL LLVPKTYIRC ETLASEGLRA 251 VRYAVNRIDA EMPATAVMKL SQSDAAGLRA FQNIFIRSRL QLIGMTHGIM 301 EYILENLERY VRNDIKFVDY ERREIRRRHQ VSEILYRYVC HSVSPVAPVA 351 HQLMEANIVK TLATEYTYAA AQMLQKLLGA KGFERGHTAG NIAIDIRPFT 401 IFEGPNDMLY AEIYDQFVRA TAEEKEAGMK LDKNQTLLDR LQTDARFAAV 451 ARDYTLPEDI RSFLQEHTLT DACALQKVFI GKIIARLFVF VQAKHEDTAA 501 FLLNDIRKDI LDCRYCG* m644/g644 94.6% identity in 517 aa overlap 10 20 30 40 50 60 m644.pep MPSERSADCCPAHFVVKFRKSTLNCGRRFDRPPINGNRQRKPMIHTEPSAQPSTMDTAAF I I I I I I I I I I : I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g644 MPSERPADCCPVHFVVKFRKLTLNCGRRFDRPPINGNRQRKPMIHTEPSAQPΞTMDTAAF 10 20 30 40 50 60 70 80 90 100 110 120 m644. ep LKHIESAFRRIFSDGIDLMRYLPEDKWLALKQAGLLLPFLDKKYGGRKGSQFEIQEVLRI llllllll I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I g644 LKHIESAFPRIFSDGIDLMRYLPEDKWLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRI 70 80 90 100 110 120 130 140 150 160 170 180 m644. pep AGHYGVPVTLRTGIEGALVLQPLQEFGDEAQVAQGLEMIFKGEGGGLGVTEPETSGAAIA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I : llllllllllllll g64 AGHYGVPVTLRTGIEGALVLQPLQEFGGEAQVAQGLDMIFKGESRRLGVTEPETSGAAIA 130 140 150 160 170 180 190 200 210 220 230 240 m644.pep REMQSYYEYIDGQTIYVNAAKYWQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRC I I I I I III I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g644 REMQSCYEYTDEQTIYVNAAKYWQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRC 190 200 210 220 230 240 250 260 270 280 290 300 m644.pep ETLASEGLRAVRYAVNRIDAEMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIM I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I :: I I I I I I I I I I I I I I I I I I I I I I I I I I I g644 ETLASEGLRAVRYAVNRIDAEMPATAVMKLSRGDAAGLRAFQNIFIRSRLQLIGMTHGIM 250 260 270 280 290 300 310 320 330 340 350 360 m64 .pep EYILENLERYVRNDIKFVDYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVK II I I : I I : I I I I I I I :| I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g644 EYILDNLNRYVRNDIRFVDYERREIQRRHQVΞEILYRYVCHSVΞPVAPVAHQLMEANIVK 310 320 330 340 350 360 370 380 390 400 410 420 ra644.pep TLATEYTYAAAQMLQKLLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g64 TLATEYTYAAAQMLQKLLGAKGFERGHPAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRA 370 380 390 400 410 420 430 440 450 460 470 480 m6 4.pep TAEEKEAGMKLDKNQTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFI I I I I I I I I : I I I I I I I I I I : I I I : I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I g644 TAEEKEAGIKLDKNQTLLDAVQTDVRFAAVARDYALPEDIRSFLQEHTLTDACALQKVFI 430 440 450 460 470 480 490 500 510 m644.pep GKIIARLFVFVQAKHEDTAAFLLNDIRKDILDCRYCGX I I I I I I I I I I I I : I I I I : I I I I I I I I I I I I I I I I I I I g644 GKIIARLFVFVQEEHEDTTAFLLNDIRKDILDCRYCGX 490 500 510 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2073>: a644. seq 1 ATGCCGTCTG AAAGGTCGGC GGATTGTTGC CCGGCGCACT TTGTGGTAAA 51 GTTTAGAAAA TCAACTCTAA ACTGTGGCAG GCGGTTTGAC CGGCCGCCGA 101 TTAATGGGAA CCGACAGAGG AAGCCGATGA TACACACCGA ACCGAGCGCG 151 CAGCCGTCAA CTATGGACAC GGCTGCTTTT TTAAAGCACA TCGAATCCGC 201 ATTCCGCCGC ATTTTTGCAG ACGGTATCGA CCTGATGCGA TACCTGCCCG 251 AAGACAAATG GCTTGCCTTG AAGCAGGCGG GTTTGCTGTT GCCCTTCCTC 301 GACAAAAAAT ACGGCGGGCG CAAGGGCAGC CAGTTTGAAA TTCAGGAAGT 351 CTTGCGGATT GCGGGGCATT ACGGCGTGCC CGTTAΝΝΝΝΝ ΝΝΝΝΝΝΝΝΝΝ 401 ΝΝGAAGGCGC GCTGGTGTTG CAGCCACTGC AAGAGTTCGG CGATGAAGCG 451 CAAATCGCAC AGGGTTTGGA CATGGTTTTC AAAGGCGAGG GCGGCGGTTT 501 AGGCGTTACC GAACCCGAAA CCTCCGGCGC GGCGATTGCC CGAGAAATGC 551 AGTCTTACTA CGAATATACC GACGGACAAA CCATTTACGT CAACGCCGCG 601 AAATACTGGC AGGGCAACTC GCAAAGCGAC TTCCTCCTCG TTGCCGCCAA 651 AGAGCGCAAA AACGGCAAAC TCGCCAAAGT CATCGACCTG CTGCTCGTCC 701 CCAAAACATA CATCCGCTGC GAAACCCTCG CATCCGAAGG CTTGCGCGCC 751 GTCCGTTACG CCGTCAACCG CATCGATGCC GAAATGCCTG CAACCGCCGT 801 GATGAAACTC TCCCAGAGCG ACGCTGCCGG TTTGCGCGCG TTCCAAAACA 851 TCTTTATCCG CAGCCGCCTG CAACTGATCG GCATGACGCA CGGCATTATG 901 GAATACACCC TTGAAAACCT GGAACGATAC GTCCGCAACG ACATCAGATT 951 CGTCGATTAC GAACGCCGCG AAATCCGGCG CCGCCATCAG GTTTCCGAGA 1001 TTCTTTACCG CTACGTCTGC CATTCCGTTT CGCCCGTTGC ACCCGTCGCC 1051 CATCAACTGA TGGAGGCGAA CATCGTCAAA ACCCTCGCCA CGGAATACAC 1101 TTACGCCGCC GCGCAAATGT TGCAAAAACT CTTGGGCGCG AAGGGTTTTG 1151 AACGCGGACA CACCGCCGGC AATATCGCTA TCGACATCCG CCCCTTCACG 1201 ATTTTTGAAG GCCCGAACGA TATGCTTTAT GCCGAAATTT ACGACCAGTT 1251 TGTCCGCGCC ACCGCCGAAG AAAAAGAAGC AGGCATGAAG TTGGACAAAA 1301 ACCAAACCCT GCTCGACCGC CTGCAAACCG ATGCCCGCTT TGCCGCCGTC 1351 GCCCGCGACT ACACTTTGCC CGAAGACATC CGCAGCTTCC TGCAGGAACA 1401 CACCCTGACC GATGCCTGCG CCCTGCAAAA AGTCTTTATC GGCAAAATCA 1451 TCGCCCGACT CTTTGTCTTC GTACAGGCGG AACACGAAGA CACCGCAGCC 1501 TTCCTGCTGA ACGACATCCG CAAAGACATA TTGGACTGCC GATATTGCGG 1551 ATAG This corresponds to the amino acid sequence <SEQ ID 2074; ORF 644.a>: a644.pep 1 MPSERSADCC PAHFWKFRK STLNCGRRFD RPPINGNRQR KPMIHTEPSA 51 QPSTMDTAAF LKHIESAFRR IFADGIDLMR YLPEDKWLAL KQAGLLLPFL 101 DKKYGGRKGS QFEIQEVLRI AGHYGVPVXX XXXXEGALVL QPLQEFGDEA 151 QIAQGLDMVF KGEGGGLGVT EPETSGAAIA REMQSYYEYT DGQTIYVNAA 201 KYWQGNSQSD FLLVAAKERK NGKLAKVIDL LLVPKTYIRC ETLASEGLRA 251 VRYAVNRIDA EMPATAVMKL SQSDAAGLRA FQNIFIRSRL QLIGMTHGIM 301 EYTLENLERY VRNDIRFVDY ERREIRRRHQ VSEILYRYVC HSVSPVAPVA 351 HQLMEANIVK TLATEYTYAA AQMLQKLLGA KGFERGHTAG NIAIDIRPFT 401 IFEGPNDMLY AEIYDQFVRA TAEEKEAGMK LDKNQTLLDR LQTDARFAAV 451 ARDYTLPEDI RSFLQEHTLT DACALQKVFI GKIIARLFVF VQAEHEDTAA 501 FLLNDIRKDI LDCRYCG* m644/a644 97.3% identity in 517 aa overlap 10 20 30 40 50 60 m644.pep MPSERSADCCPAHFWKFRKSTLNCGRRFDRPPINGNRQRKPMIHTEPSAQPSTMDTAAF II II II II II II I I I I I I II I I II I II II I II I II II II II II I II II II II II II II II a644 MPSERSADCCPAHFWKFRKSTLNCGRRFDRPPINGNRQRKPMIHTEPSAQPSTMDTAAF 10 20 30 40 50 60 70 80 90 100 110 120 m644.pep LKHIESAFRRIFSDGIDLMRYLPEDKWLALKQAGLLLPFLDKKYGGRKGSQFEIQEVLRI MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a64 LKHIESAFRRIFADGIDLMRYLPEDKWLALKQAGLLLPFLDKKYGGRKGSQFEIQEVLRI 70 80 90 100 110 120 130 140 150 160 170 180 m644. pep AGHYGVPVTLRTGIEGALVLQPLQEFGDEAQVAQGLEMIFKGEGGGLGVTEPETSGAAIA II II I II I : : II II II II II II II II I : II II : I : II I II II II II II II II I II I a644 AGHYGVPVXXXXXXEGALVLQPLQEFGDEAQIAQGLDMVFKGEGGGLGVTEPETSGAAIA 130 140 150 160 170 180 190 200 210 220 230 240 m644. pep REMQSYYEYIDGQTIYVNAAKYWQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRC I II II II II II I I II I II I II II II I II II II I II II II I II II II II II II II II II I a644 REMQSYYEYTDGQTIYVNAAKYWQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRC 190 200 210 220 230 240 250 260 270 280 290 300 m644.pep ETLASEGLRAVRYAVNRIDAEMPATAVMKLSQSDAAGLRAFQNIFIRΞRLQLIGMTHGIM MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a644 ETLASEGLRAVRYAVNRIDAEMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIM 250 260 270 280 290 300 310 320 330 340 350 360 m644. pep EYILENLERYVRNDIKFVDYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVK M I II II II II II I : I II II I I I I I I I II I I I II I II II I I II I II I I II II I II I I II a644 EYTLENLERYVRNDIRFVDYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVK 310 320 330 340 350 360 370 380 390 400 410 420 m644.pep TLATEYTYAAAQMLQKLLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRA MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a644 TLATEYTYAAAQMLQKLLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRA 370 380 390 400 410 420 430 440 450 460 470 480 m644. pep TAEEKEAGMKLDKNQTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFI II I I I II II II II II II II II II II I II II II I II II II I II II II II II II II II II II a64 TAEEKEAGMKLDKNQTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFI 430 440 450 460 470 480 490 500 510 m644.pep GKIIARLFVFVQAKHEDTAAFLLNDIRKDILDCRYCGX I I II I I I I I II I I : II II II II I I II II II II II II II a644 GKIIARLFVFVQAEHEDTAAFLLNDIRKDILDCRYCGX 490 500 510 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2075>: g645.seq 1 ATGATGATGG TGTTGGCGTT GGGGATGTCG ATGCCGGTTT CGATGATGGT 51 GGAACAGAGC AACACATTGA ATCTTTGCTG CAAAAAGTCG CGCATGACTT 101 GTTCCAGCTC GCGCTCACGC AGTTGTCCGT GCGCCACGCC GATACGGGCT 151 TCGGGCAGCA GGGTTTCCAG CCGCTCGCGC ATATTCTCAA TCGTATCTAC 201 TTCATTGTGC AGGAAAAata cCTGTCCTCC GCGTTTGAGT TCGCGCAACA 251 CGGCTTCGCG CACGCTGCCT TCGCTGAACG GTTTGACAAA GGTTTTCACG 301 GCGAGGCGGC GGCTCGGTGC AGTGGTAATC AGCGAGAAGT CGCGCAGACC 351 TTCGAGCGCC ATGCTGAGGG TGCGCGGAAT CGGCGTGGCG GTCATGGTTA 401 GGATGTCGAC ATTGGCGCGC AGGCGTTTGA GCTGCTCTTT CTGTCGCACG 451 CCGAAGCGGT GTTCTTCATC GATAATCAAT AAACCTAAGT TTTTGAATTT 501 TATGTCGTCC TGCACCAATT TGTGCGTACC GATAACGATA TCGACAGTAC 551 CGTCCGCCAT GCCTTCGAGC GTGGCTTTGG TGGCTTTGCT GTTGTTGAAA 601 CGCGAAAGGC TGGCGACTTT CACGGGGAAA TCGGCGAAAC GGTCGGCGAA 651 GTTTTGCGCG TGCTGCTCGA CCAGAAGCGT GGTCGGGGCG AGTACGGCGA 701 CCTGTTTGCC GCCCATCACC GCCACAAACG CGGCGCGAAG GGCGACTTCG 751 GTTTTGCCGA AACCGACATC GCCGCACACA AGTCGGTCCA TCGGCTTCGC 801 CTGCGTCAAA TCTTTAATCA CGGcggcgat ggcggcggcC TGGTCTTCGG 851 TTTCCTCGTA G This corresponds to the amino acid sequence <SEQ ID 2076; ORF 645. ng>: g645.pep 1 MMMVLALGMS MPVSMMVEQS ΝTLΝLCCKKS RMTCSSSRSR SCPCATPIRA 51 SGSRVSSRΞR IFΞIVSTSLC RKΝTCPPRLS SRΝTAΞRTLP ΞLΝGLTKVFT 101 ARRRLGAVVI SEKSRRPΞSA MLRVRGIGVA VMVRMΞTLAR RRLSCSFCRT 151 PKRCSSΞIIΝ KPKFLΝFMSS CTΝLCVPITI STVPΞAMPSS VALVALLLLK 201 RERLATFTGK SAKRSAKFCA CCSTRSVVGA STATCLPPIT ATΝAARRATS 251 VLPKPTSPHT SRSIGFACVK ΞLITAAMAAA WΞΞVSS* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2077>: m645. seq 1 ATGATGATGG TGTTGGCGTT GGGGATATCG ATACCGGTTT CGATGATGGT 51 GGAACAGAGC AACACGTTAA ATCGTTGCTG CAAAAAGTCG CGCATGACTT 101 GTTCCAGCTC GCGCTCGCGC AGTTGTCCGT GCGCCACGCC GATGCGGGCT 151 TCGGGCAGCA GGGTTTCCAG CCGCTCGCGC ATATTTTCAA TCGTATCTAC 201 TTCATTGTGC AGGAAAAATA CCTGTCCTCC GCGTTTGAGT TCGCGCAACA 251 CGGCTTCGCG CACGCTGCCT TCGCTAAAGG GTTTGACAAA GGTTTTGACG 301 GCGAGGCGGC GGCTGGGCGC GGTGGTAATC AGCGAGAAGT CGCGCAGTCC 351 TTCCAACGCC ATACTTAAAG TACGCGGAAT CGGCGTGGCG GTCATGGTAA 401 GGATATCAAC ATTGGCGCGC AGGCGTTTGA GCTGCTCTTT CTGACGCACG 451 CCGAAGCGGT GTTCTTCGTC GATAATCACT AAACCTAAGT TTTTGAATTT 501 GATGTCGTCC TGCACCAGTT TGTGCGTACC GATAACAATA TCGACCGTGC 551 CGTCTGCCAT GCCTTCCAGC GCGGCTTTGG TGGCTTTGCT GTTGTTGAAA 601 CGCGAAAGGC TGGCGACTTT CACGGGGAAA TCGGCGAAAC GGTCGGCGAA 651 GTTTTGCGCG TGCTGCTCGA CCAAAAGCGT GGTCGGAGCA AGTACGGCGA 701 CCTGTTTGCC GCCCATCACC GCCACAAACG CGGCGCGCAG GGCGACTTCG 751 GTTTTGCCGA AGCCGACATC GCCGCACACA AGGCGATCCA TCGGCTTCGC 801 TTGCGTCAAA TCTTTAATCA CGGCGGCGAT GGCGGCGGCC TGGTCTTCGG 851 TTTCCTCGTA G This corresponds to the amino acid sequence <SEQ ID 2078; ORF 645>: m645.pep 1 MMMVLALGIS IPVSMMVEQS NTLNRCCKKS RMTCSSSRSR SCPCATPMRA 51 SGSRVSSRSR IFΞIVSTSLC RKNTCPPRLS SRNTASRTLP SLKGLTKVLT 101 ARRRLGAVVI SEKSRΞPSNA ILKVRGIGVA VMVRISTLAR RRLSCSF*RT 151 PKRCSSSIIT KPKFLNLMSS CTSLCVPITI STVPSAMPSS AALVALLLLK 201 RERLATFTGK SAKRSAKFCA CCSTKSVVGA STATCLPPIT ATNAARRATS 251 VLPKPTSPHT RRSIGFACVK SLITAAMAAA WSSVSS* 645/g645 93.7% identity in 286 aa overlap 10 20 30 40 50 60 m645.pep MMMVLALGISIPVSMMVEQSNTLNRCCKKSRMTCSSSRSRSCPCATPMRASGSRVSSRSR I I I I I I I I : Ml I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I II g645 MMMVLALGMSMPVSMMVEQSNTLNLCCKKSRMTCSSSRSRSCPCATPIRASGSRVSSRSR 10 20 30 40 50 60 70 80 90 100 110 120 m645.pep IFSIVSTSLCRKNTCPPRLSSRNTASRTLPΞLKGLTKVLTARRRLGAVVISEKSRSPSNA II II I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I : I I I I I I I I I I I I I I I I I M I g645 IFSIVSTSLCRKNTCPPRLSSRNTASRTLPSLNGLTKVFTARRRLGAVVISEKSRRPSSA 70 80 90 100 110 120 130 140 150 160 170 180 m645.pep ILKVRGIGVAVMVRISTLARRRLSCSFXRTPKRCSSSIITKPKFLNLMSSCTSLCVPITI : I : I I I I I I I I II I : I II I I I I I I II I I I I I I I I I I I I : I I I I I I : I I I I I : I I I I I I I g645 MLRVRGIGVAVMVRMΞTLARRRLSCSFCRTPKRCΞSSIINKPKFLNFMSSCTNLCVPITI 130 140 150 160 170 180 190 200 210 220 230 240 m645.pep STVPSAMPSΞAALVALLLLKRERLATFTGKSAKRSAKFCACCSTKSVVGASTATCLPPIT I I I II I I I I I :| I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I : I II I I I I I I I I I I I I g6 5 STVPSAMPSSVALVALLLLKRERLATFTGKΞAKRSAKFCACCSTRSVVGASTATCLPPIT 190 200 210 220 230 240 250 260 270 280 m645.pep ATNAARRATSVLPKPTSPHTRRSIGFACVKSLITAAMAAAWSΞVSSX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g6 5 ATNAARRATSVLPKPTSPHTSRSIGFACVKSLITAAMAAAWSΞVSΞX 250 260 270 280 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2079>: a645. seq 1 ATGATGATGG TGTTGGCGTT GGGAATGTCG ATACCGGTTT CGATGATGGT 51 GGAACAGAGC AACACGTTAA ATCGTTGCTG CAAAAAGTCG CGCATGACTT 101 GTTCCAGCTC GCGCTCGCGC AGTTGTCCGT GCGCCACGCC GATGCGGGCT 151 TCGGGCAGCA GGGTTTCCAG CCGCTCACGC ATGTTTTCGA TGGTATCCAC 201 TTCATTGTGC AGGAAAAATA CTTGCCCGCC GCGTTTGAGT TCGCGCAATA 251 CGGCTTCGCG CACGCTGCCT TCGCTGAACG GTTTGACAAA GGTTTTGACG 301 GCGAGGCGGC GGCTGGGCGC AGTGGTAATC AGCGAGAAGT CGCGCAGTCC 351 TTCCAGCGCC ATACTTAAAG TACGCGGAAT CGGCGTAGCG GTCATGGTAA 401 GGATGTCGAC ATTGGCGCGC AGGCGTTTGA GCTGCTCTTT CTGACGCACG 451 CCGAAGCGGT GTTCTTCGTC GATAATCACT AAACCTACGT TTTTGAATTT 501 TATGTCGTCC TGCACCAGTT TGTGCGTACC GATAACAATA TCGACCGTGC 551 CGTCCGCCAT GCCTTCCAGC GCGGCTTTGG TGGCTTTGCT GTTGTTGAAA 601 CGCGAAAGGC TGGCGACTTT CACGGGGAAA TCGGCGAAGC GGTCGGCAAA 651 ATTTTGCGCG TGCTGCTCGA CCAGAAGCGT GGTCGGTGCG AGTACGGCAA 701 CTTGTTTGCC ACCCATTACC GCCACAAACG CGGCGCGCAG GGCGACTTCG 751 GTTTTGCCGA AACCGACATC GCCGCACACG AGGCGGTCCA TCGGCTTCGC 801 CTGCGTCAAA TCTTTAATCA CGGCGGCGAT GGCGGCTGCC TGGTCTTCGG 851 TTTCTTCGTA G This corresponds to the amino acid sequence <SEQ ID 2080; ORF 645. a>: a645.pep 1 MMMVLALGMS IPVSMMVEQS ΝTLΝRCCKKS RMTCSSSRSR SCPCATPMRA 51 SGSRVSSRSR MFSMVSTSLC RKNTCPPRLS SRNTASRTLP SLNGLTKVLT 101 ARRRLGAVVI SEKSRSPSSA ILKVRGIGVA VMVRMSTLAR RRLSCSF*RT 151 PKRCSSSIIT KPTFLNFMSS CTSLCVPITI STVPSAMPSS AALVALLLLK 201 RERLATFTGK SAKRSAKFCA CCSTRSWGA STATCLPPIT ATNAARRATS 251 VLPKPTSPHT RRSIGFACVK SLITAAMAAA WSSVSS* m645/a645 96.9% identity in 286 aa overlap 10 20 30 40 50 60 m645 . pep MMMVLALGISIPVSMMVEQSNTLNRCCKKSRMTCSSSRSRSCPCATPMRASGSRVSSRSR M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M a645 MMMVLALGMSIPVSMMVEQSNTLNRCCKKSRMTCSSSRSRSCPCATPMRASGSRVSSRSR 10 20 30 40 50 60 70 80 90 100 110 120 m645.pep IFSIVSTSLCRKNTCPPRLSSRNTASRTLPSLKGLTKVLTARRRLGAVVISEKSRSPSNA : I I : I II I I I II II I II II I II II II II II I M II I II II II I II II II II II II II I : I a6 5 MFSMVSTSLCRKNTCPPRLSSRNTASRTLPSLNGLTKVLTARRRLGAWISEKSRSPSSA 70 80 90 100 110 120 130 140 150 160 170 180 m645.pep ILKVRGIGVAVMVRISTLARRRLSCSFXRTPKRCSSSIITKPKFLNLMSSCTSLCVPITI II I II I I II II I I I : II II II II II I I I I I I II I II II II I I II I : II II II I II I I I I a645 ILKVRGIGVAVMVRMSTLARRRLSCSFXRTPKRCSSSIITKPTFLNFMSSCTSLCVPITI 130 140 150 160 170 180 190 200 210 220 230 240 m645. pep STVPSAMPSSAALVALLLLKRERLATFTGKSAKRSAKFCACCSTKSWGASTATCLPPIT II I II II II I II II I I II II II II II I II II II II II II II II I : I II I II II I II II II a645 STVPSAMPSSAALVALLLLKRERLATFTGKSAKRSAKFCACCSTRSWGASTATCLPPIT 190 200 210 220 230 240 250 260 270 280 m645.pep ATNAARRATSVLPKPTSPHTRRSIGFACVKSLITAAMAAAWSSVSSX II I I II II II II II II II II II II II II I II I II II II II I II II II a645 ATNAARRATSVLPKPTSPHTRRSIGFACVKSLITAAMAAAWSSVSSX 250 260 270 280 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2081>: g647. seq 1 ATGCAAAGGC TCGCCGCAGA CGGCATCCAA ATCTTTTTTG TAGGTGTCGA 51 TGGGCAGTTT GCCTTGCGAA TAAACGGTTT GGTTAAAGAG CGTGCACGCT 101 CCGTATTCTT TGGCAAGGTT TGCCGATGCT TTGAGCAGGT AATACTGTAT 151 GGCTTCAAAG GTACGGTGGG TCAGACCGAG CGCGGAACCG TCGCTGTAGC 201 GGACACCGTT TTTCGCCAGA TAGTAGGCGT AGTTGATGAC ACCGATGCCG 251 AGCGAACGGC GGTCCATAGT AGAGGTACGC GCGGCTTCTA CCGGATATCC 301 CTGATAATCT AA This corresponds to the amino acid sequence <SEQ ID 2082; ORF 647.ng>: g647.pep 1 MQRLAADGIQ IFFVGVDGQF ALRIΝGLVKE RARSVFFGKV CRCFEQVILY 51 GFKGTVGQTE RGTVAVADTV FRQIVGVVDD TDAERTAVHS RGTRGFYRIS 101 LII* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2083>: m647. seq 1 ATGCAAAGGC TCGCCGCAGA CGGCATCCAA ATCTTTTTTG TAAGTGTCGA 51 TGGGCAGTTT GCCTTGCGAA TAAACGGTTT GGTTAAAGAG CGTGCACGCA 101 CCGTATTCTT TGGCAAGGTT TGCCGATGCT TTGAGCAGGT AATACTGTAT 151 GGCTTCAAAG GTACGGTGGG TCAGACCGAG CGCGGAACCG TCGCTGTAGC 201 GGACACCGTT TTTCGCCAGA TAATAAGCAT AGTTAATCAC GCCGATGCCG 251 AGCGAACGGC GGCCCATAGT AGAGGTACGC GCGGCTTCTA CCGGATATCC 301 CTGATAATCT AA This corresponds to the amino acid sequence <SEQ ID 2084; ORF 647>: 6 7.pep 1 MQRLAADGIQ IFFVSVDGQF ALRIΝGLVKE RARTVFFGKV CRCFEQVILY 51 GFKGTVGQTE RGTVAVADTV FRQIISIVNH ADAERTAAHS RGTRGFYRIS 101 LII* m647/g647 91.3% identity in 103 aa overlap 10 20 30 40 50 60 m647.pep MQRLAADGIQIFFVSVDGQFALRINGLVKERARTVFFGKVCRCFEQVILYGFKGTVGQTE I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I l I I I I I I I I I I I I I I I I I II I I I I I I g647 MQRLAADGIQIFFVGVDGQFALRINGLVKERARSVFFGKVCRCFEQVILYGFKGTVGQTE 10 20 30 40 50 60 70 80 90 100 m647.pep RGTVAVADTVFRQIISIVNHADAERTAAHSRGTRGFYRISLIIX I I I I I I I I I I I I I I ::: I : : I I I I I I : I I I I I I I I I I I I I I I I g647 RGTVAVADTVFRQIVGVVDDTDAERTAVHΞRGTRGFYRISLIIX 70 80 90 100 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2085>: a647. seq 1 GTGCAAAGGC TCGTTACACA CAGCGTCCAA GTCTTTTTTG TAGGTGTCGA 51 TGGGCAGTTT GCCTTGCGAA TAAACGGTTT GGTTAAAGAG CGTGCACGCA 101 CCGTATTCTT TGGCAAGGTT TGCCGATGCT TTGAGCAGGT AATACTGTAT 151 GGCTTCAAAG GTACGGTGGG TCAGACCGAG CGCGGAGCCG TCGCTGTAGC 201 GGACACCGTT TTTCGCCAAA TAATACGCAT AGTTGATCAC GCCGATACCG 251 AGCGAACGGC GGCCCATAGT GGAGGTACGC GCGGCTTCTA CCGGATATCC 301 CTGATAATCT AA This corresponds to the amino acid sequence <SEQ ID 2086; ORF 647.a>: a647.pep 1 VQRLVTHSVQ VFFVGVDGQF ALRIΝGLVKE RARTVFFGKV CRCFEQVILY 51 GFKGTVGQTE RGAVAVADTV FRQIIRIVDH ADTERTAAHS GGTRGFYRIS 101 LII* m647/a647 87.4% identity in 103 aa overlap 10 20 30 40 50 60 m647. pep MQRLAADGIQIFFVSVDGQFALRIΝGLVKERARTVFFGKVCRCFEQVILYGFKGTVGQTE M:: :: I : I I I : I I II II II II I II II II II II II II I II II II II II II I II II II a647 VQRLVTHSVQVFFVGVDGQFALRIΝGLVKERARTVFFGKVCRCFEQVILYGFKGTVGQTE 10 20 30 40 50 60 70 80 90 100 m647.pep RGTVAVADTVFRQIISIVΝHADAERTAAHSRGTRGFYRISLIIX II : II I I II II II II I I : II I : II II II I II II II II I II II a647 RGAVAVADTVFRQIIRIVDHADTERTAAHSGGTRGFYRISLIIX 70 80 90 100 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 2087>: g648.seq 1 ATGAACAGGC GCAACGCGCG GATCGAACGG GCTGTGCGTA TTGCAGTGAT 51 CGACGTTTTG AATGTAGATG CGCCCGGTCC CGGCACGCTC CTGCATCAGC 101 GTGGAAAACA GGTCGGCAGC CGGAATGATA CGCTTGCGTA TGTTCGGGTC 151 TTGCTCGTAT TTCGTATAGA GCCGCTCAAA TTCGTCTTGG TCGGCAAAAA 201 ACGCTTCGTA CAACCCCGAA ACCTCGTTGG GCGAAAACAG CGTAATGTTG 251 CCGCCCTTAA TCAGGCGGGT GTACAGCAGG CGGTTGATTT GCACGCCATA 301 ATCAAGCTGG CGGATACGGT TGTCTTCCAC GCCCCGGTTG TTTTTCAACA 351 CCAGCAGGCT TTCGGCTTCA ATATGCCACA AGGGGTAGAA CAAGGTTGCC 401 GCGCCGCCGC GCACGCCACC TTGCGAACAA GATTTGACCG CCGCCTGAAA 451 CATCTTAAAG AAGGGAATGC AGCCGGTATG CCGGGCTTCA CCGCCCCGGA 501 TTTCGCTGTC CAGCCCGCGG ATACGTCCGG CATTGATGCC GATGCCCGCG 551 CGTTGGGAAA CGTATTTCAC AATCGCGCTG GTAGTGGCAT TGATGGAATC 601 CAAACTATCG TCGCATTCAA TCAGCACACA GCTTGA This corresponds to the amino acid sequence <SEQ ID 2088; ORF 648.ng>: g648 .pep 1 MNRRNARIER AVRIAVIDVL NVDAPGPGTL LHQRGKQVGS RNDTLAYVRV 51 LLVFRIEPLK FVLVGKKRFV QPRNLVGRKQ RNVAALNQAG VQQAVDLHAI 101 IKLADTVVFH APVVFQHQQA FGFNMPQGVE QGCRAAAHAT LRTRFDRRLK 151 HLKEGNAAGM PGFTAPDFAV QPADTSGIDA DARALGNVFH NRAGSGIDGI 201 QTIVAFNQHT A* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2089>: m648.seq 1 ATGAACAGGC GCGACGCGCG GATCGAACGG GCTGTGCGTA TTGCAGTGAT 51 CGACGTTTTG AATGTAGATG CGCCCGGTTC CGGCACGCTC CTGCATCAGC 101 GTGGAAAACA GGTCGGCAGC CGGAATGATG CGCTTGCGGA TATCAGGGTC 151 TTGCTCGTAT TTCGTATAGA GCCGCTCAAA TTCGTCTTGG TCGGCAAAAA 201 ACGCTTCGTA CAATCCCGGA ACCTCGTTGG GCGAAAACAG CGTAATGTTG 251 CCGCCCTTAA TCAGGCGGGT GTACAGCAGG CGGTTGATTT GCACGCCGTA 301 ATCAAGCTGA CGGATACGGT TGTCTTCCAC ACCGCGGTTG TTTTTCAACA 351 CCAGCAGGCT TTCGGCTTCG ATATGCCACA AGGGGTAGAA CAAGGTTGCC 401 GCGCCGCCGC GCACGCCGCC TTGCGAACAG GATTTGACCG CCGCCTGAAA 451 CATTTTAAAG AAGGGAATGC AGCCGGTATG CCGCGCTTCG CCGCCCCGGA 501 TTTCGCTGTC CAAACCGCGG ATACGTCCGG CATTGATGCC GATGCCCGCA 551 CGCTGGGAAA CGTATTTCAC AATCGCGCTG GTAGTGGCAT TGATGGAATC 601 CAAACTATCG TCGCATTCAA TCAGCACACA GCTTGA This corresponds to the amino acid sequence <SEQ ID 2090; ORF 648>: m648.pep 1 MΝRRDARIER AVRIAVIDVL ΝVDAPGSGTL LHQRGKQVGS RΝDALADIRV 51 LLVFRIEPLK FVLVGKKRFV QSRΝLVGRKQ RΝVAALΝQAG VQQAVDLHAV 101 IKLTDTVVFH TAVVFQHQQA FGFDMPQGVE QGCRAAAHAA LRTGFDRRLK 151 HFKEGΝAAGM PRFAAPDFAV QTADTSGIDA DARTLGΝVFH NRAGSGIDGI 201 QTIVAFNQHT A* 648/g648 91.5% identity in 211 aa overlap 10 20 30 40 50 60 m6 8.pep MNRRDARIERAVRIAVIDVLNVDAPGSGTLLHQRGKQVGSRNDALADIRVLLVFRIEPLK I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I : I I I I I I I I I I I I g648 MNRRNARIERAVRIAVIDVLNVDAPGPGTLLHQRGKQVGSRNDTLAYVRVLLVFRIEPLK 10 20 30 40 50 60 70 80 90 100 110 120 m648.pep FVLVGKKRFVQSRNLVGRKQRNVAALNQAGVQQAVDLHAVIKLTDTVVFHTAVVFQHQQA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I : I I I I I I : llllllll g648 FVLVGKKRFVQPRNLVGRKQRNVAALNQAGVQQAVDLHAIIKLADTVVFHAPVVFQHQQA 70 80 90 100 110 120 130 140 150 160 170 180 m648.pep FGFDMPQGVEQGCRAAAHAALRTGFDRRLKHFKEGNAAGMPRFAAPDFAVQTADTSGIDA I I I : I I I I I I I I I II I I I I : I I I I I I I I I I : I I I I I I I I I I :| I I I I I I llllllll g648 FGFNMPQGVEQGCRAAAHATLRTRFDRRLKHLKEGNAAGMPGFTAPDFAVQPADTSGIDA 130 140 150 160 170 180 190 200 210 m648.pep DARTLGNVFHNRAGSGIDGIQTIVAFNQHTAX I I M I I I I I I I I I I I I I I I I I I I I I I I I I I I I g6 8 DARALGNVFHNRAGSGIDGIQTIVAFNQHTAX 190 200 210 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2091>: a648. seq 1 ATGAACAGGC GCAACGCGCG GATCGAACGG GCTGTGCGTA TTGCAGTGAT 51 CGACGTTTTG AATGTAGATG CGCCCGGTTC CGGCACGCTC CTGCATCAGC 101 GTGGAAAACA GGTCGGCAGC CGGAATGATG CGCTTGCGGA TATCAGGGTC 151 TTGCTCGTAT TTCGTATAGA GCCGCTCAAA TTCGTCTTGG TCGGCAAAAA 201 ACGCTTCGTA CAATCCCGGA ACCTCGTTGG GCGAAAACAG CGTAATGTTG 251 CCGCCCTTAA TCAGGCGGGT GTACAGCAGG CGGTTGATTT GCACGCCGTA 301 ATCAAGCTGA CGGATACGGT TGTCTTCCAC GCCCCGGTTG TTTTTCAACA 351 CCAGCAGGCT TTCGGCTTCG ATATGCCACA AGGGGTAGAA CAAGGTTGCC 401 GCGCCGCCGC GCACGCCACC TTGCGAACAG GATTTGACTG CCGCCTGAAA 451 CATTTTAAAG AAGGGAATGC AGCCGGTATG CCGTGCTTCG CCGCCCCGGA 501 TTTCGCTGTC CAGTCCGCGG ATACGTCCGG CATTGATGCC GATGCCCGCA 551 CGCTGGGAAA CGTATTTCAC AATCGCGCTG GTAGTGGCGT TGATGGAATC 601 CAGGCTGTCG TCGCATTCGA TCAATACGCA GCTTGA This corresponds to the amino acid sequence <SEQ ID 2092; ORF 648. a>: a648.pep 1 MNRRNARIER AVRIAVIDVL NVDAPGSGTL LHQRGKQVGS RNDALADIRV 51 LLVFRIEPLK FVLVGKKRFV QSRNLVGRKQ RNVAALNQAG VQQAVDLHAV 101 IKLTDTWFH APWFQHQQA FGFDMPQGVE QGCRAAAHAT LRTGFDCRLK 151 HFKEGNAAGM PCFAAPDFAV QSADTSGIDA DARTLGNVFH NRAGSGVDGI 201 QAWAFDQYA A* m648/a648 93.8% identity in 211 aa overlap 10 20 30 40 50 60 m648. pep MNRRDARIERAVRIAVIDVLNVDAPGSGTLLHQRGKQVGSRNDALADIRVLLVFRIEPLK II I I : I I I I I II I I II II II II II I II II II II II II II II II II II II II II II II II I a648 MNRRNARIERAVRIAVIDVLNVDAPGSGTLLHQRGKQVGSRNDALADIRVLLVFRIEPLK 10 20 30 40 50 60 70 80 90 100 110 120 m648. pep FVLVGKKRFVQSRNLVGRKQRNVAALNQAGVQQAVDLHAVIKLTDTWFHTAWFQHQQA II I I I II II II II II II II II II II II I I I I I II II I II II I I I I II I II : llllllll a 648 FVLVGKKRFVQSRNLVGRKQRNVAALNQAGVQQAVDLHAVIKLTDTWFHAPWFQHQQA 70 80 90 100 110 120 130 140 150 160 170 180 m648. pep FGFDMPQGVEQGCRAAAHAALRTGFDRRLKHFKEGNAAGMPRFAAPDFAVQTADTSGIDA I II II II II II II II II II : II I II I II I II II II II II I MMMMMMMMM a648 FGFDMPQGVEQGCRAAAHATLRTGFDCRLKHFKEGNAAGMPCFAAPDFAVQSADTSGIDA 130 140 150 160 170 180 190 200 210 m648.pep DARTLGNVFHNRAGSGIDGIQTIVAFNQHTAX II I I I I II II I I II II : I II MM II : I : : II a648 DARTLGNVFHNRAGSGVDGIQAWAFDQYAAX 190 200 210 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2093>: g649. seq 1 ATGCTTGCCA TACTGTTGTC TGCAATACTG GGACTGGTAT CAACAACTGC 51 CGCTGCCGGT ACGTCAGAAC CCGCCCACCG ACATACCAAA CATATCAGCA 101 AGGCAAACAA GCAGATGCTG CACCCCGAAT GCAGGAAATA TTTGGAACGC 151 CGTGCCGCGT GGTACCGATC GCAAGGCAAC GTGCAGGAAT TGCGCGAAAA 201 CAAAAAGGCG CGCAAAGCAT TCCGCACCCT GCCTTATGCG GAACAGAAAA 251 TCCAATGCCG GGCGGCTTAT GAGGCTTTCG ATGATTTCGA CGGCGGCAGG 301 TTCCGCCGTT AA This corresponds to the amino acid sequence <SEQ ID 2094; ORF 649.ng>: g649.pep 1 MLAILLSAIL GLVSTTAAAG TSEPAHRHTK HISKAΝKQML HPECRKYLER 51 RAAWYRSQGΝ VQELREΝKKA RKAFRTLPYA EQKIQCRAAY EAFDDFDGGR 101 FRR* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2095>: m649.seq 1 ATGCTTGCCA TACTGTTGTC TGCAATATTG GGACTGGTAT CGACAACTGC 51 CGCTGCCGGT ACGTCAGAAC CCGCCCACCG CGATACCAAA CATATCCGCA 101 AGGCAAACAA GCAGATGCTG CACCCCGAAT GCAGGAAATA TTTGGAACGC 151 CGTGCCGCGT GGTACCGATC GCAAGGCAAC GTGCAGGAAT TGCGCGAAAA 201 CAAAAAGGCG CGCAAAGCAT TCCGCTCCCT GCCTTATGCG GAACAGAAAA 251 TCCAATGCCG GGCGGCTTAT GAGGCTTTCG ATGATTTCGA CGGCGGCAGT 301 TTCCGCCGTT AA This corresponds to the amino acid sequence <SEQ ID 2096; ORF 649>: 649.pep 1 MLAILLSAIL GLVSTTAAAG TSEPAHRDTK HIRKANKQML HPECRKYLER 51 RAAWYRSQGN VQELRENKKA RKAFRSLPYA EQKIQCRAAY EAFDDFDGGS 101 FRR* m649/g649 96.1% identity in 103 aa overlap 10 20 30 40 50 60 m649.pep MLAILLSAILGLVSTTAAAGTΞEPAHRDTKHIRKANKQMLHPECRKYLERRAAWYRSQGN II I I I I I I I I I I I I I I I I I I I I I I I I I llll I I I I I I I I I I I I I I I I I I I I I I I I I I I g649 MLAILLSAILGLVSTTAAAGTSEPAHRHTKHISKANKQMLHPECRKYLERRAAWYRSQGN 10 20 30 40 50 60 70 80 90 100 m649.pep VQELRENKKARKAFRSLPYAEQKIQCRAAYEAFDDFDGGSFRRX I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I llll g649 VQELRENKKARKAFRTLPYAEQKIQCRAAYEAFDDFDGGRFRRX 70 80 90 100 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2097>: a649. seq 1 ATGCTTGCCA TACTGTTGTC TGCAATATTG GGACTGGTAT CGACAACTGC 51 CGCTGCCGGT ACGTCAGAAC CCGCCCACCG CGATACCAAA CATATCCGCA 101 AGGCAAACAA GCAGATGCTG CACCCCGAAT GCAGGAAATA TTTGGAACGC 151 CGTGCCGCGT GGTACCGATC GCAAGGCAAC GTGCAGGAAT TGCGCGAAAA 201 CAAAAAGGCG CGCAAAGCAT TCCGCTCCCT GCCTTATAAG GAACAGAAAA 251 CCCAATGCCG GGCGGCTTAT GAGGCTTTCG ATGATTTCGA CGGCAGCAGG 301 TTCCGCCGTT AA This corresponds to the amino acid sequence <SEQ ID 2098; ORF 649. a>: a649.pep 1 MLAILLSAIL GLVSTTAAAG TSEPAHRDTK HIRKANKQML HPECRKYLER 51 RAAWYRSQGN VQELRENKKA RKAFRSLPYK EQKTQCRAAY EAFDDFDGSR 101 FRR* m649/a649 96.1% identity in 103 aa overlap 10 20 30 40 50 60 m649. pep MLAILLSAILGLVSTTAAAGTSEPAHRDTKHIRKANKQMLHPECRKYLERRAAWYRSQGN MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a649 MLAILLSAILGLVSTTAAAGTSEPAHRDTKHIRKANKQMLHPECRKYLERRAAWYRSQGN 10 20 30 40 50 60 70 80 90 100 m649. pep VQELRENKKARKAFRSLPYAEQKIQCRAAYEAFDDFDGGSFRRX I I II I II II II II I II I II III I I II I II II I II I I : llll a649 VQELRENKKARKAFRSLPYKEQKTQCRAAYEAFDDFDGSRFRRX 70 80 90 100 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2099>: g650. seq 1 ATGTCCAAAC TCAAAACCAT CGCCCTGACC GCATCAGGTC TGTCCGTTTG 51 TCCGGGTTTC CTATATGCCC AAAACACCTC ATCACACCAA GTCGGTTTAG 101 CGATTATGCG GTTAAACTCT TCAATACTCG ACCTGCCACC GACAAAACAA 151 TATTTCCAAT CCGGCAGCCT GTGGGACGAG CTGCGCCAAG GCTTCCGGAT 201 GGGCGAAGTC AATCCCGAAC TGGTACGCCG CCACGAAAGC AAATTCATCG 251 CAAGCCGCAG CTATTTCGAC AGGGTCGTCA ACCGGAGCCG ACCCTATATG 301 TACCATATCG CCAACGAAGT CAAAAAACGC AATATGCCCG CCGAAGCCGC 351 CCTGCTTCCC TTCATCGAAA GCGCGTTCGT CACCAAAGCC AAATCACACG 401 TCGGCGCATC GGGCCTGTGG CAGTTCATGC CCGCTACCGG CAGGCATTAC 451 GGCTTGGAAA AAACaccgGT TTACGacggc aggcacGacg TTtacgcaGc 501 taccgatgcc gcacTCAACT AtctGcAATA TCTCTAtggA CTGTTCGGCG 551 ACTGGCCGCT CGCCTTTGCC GCCTACAACT GGGGTGAAGG CAACGTCGGA 601 CGCGCCGTCA ACCGCGCCCG CGACCAAGGG CTCGAACCGA CCTACGAAAA 651 CCTGCGTATG CCCAACGAAA CGCGCAACTA TGTCCCCAAG CTGCTCGCCG 701 TGCGCAACAT TATTGCCACC CCCCAATCTT TCGGCATGAA TATCAGCGAC 751 ATAGACAACA AACCCTATTT TCAGGCAGTC GAACCGGGCC GTCCGCTCGA 801 caacGAagcC ATCGCCCGGC TTGCCGGCAT CACGCAAAGC GAGCTGCTCG 851 CCCTGAATCC TGCATTCAAC GTCCCCGCgt tcatCCCCAA AAAcaaacgc 901 aaacTGCTGC TTCCTGTCGC GTCCGTCCAA ACCTTccaaa gcaACTACCT 951 CAACGCCGCA CCCGACAGCC TGTTTTCATG GGAAGTCTAT ACGCCTGCCG 1001 CCAAAACCAG CCTGTCCGAC ATCTCGACGG CAACCGGCAT GAGCATTGCC 1051 GACATCAAAC GCCTCAACAA CCTGAACGGC AACCTTGTCA ACGCAGGACG 1101 CAGCATCCTT GTCGCCAAGA ACGGCAAGAC CCTTCATACG GCATCGGAat 1151 ccGTCGTTTC CATCGACATC GACAATACGC CcgacacCTa ccgttccaaT 1201 ATGCcggcag gcaCGGTGAA CGTCAGCATt gccCgaatcc aacCCgccgc 1251 cgcaCAGACA gcggacatta ccgtcgcacc tttgccgcaa gaaaccgtcc 1301 gtacgggaac ccgatcccct tgtccgcaTt accgaacccg ccctTGCGAC 1351 AGCCGCAGCg CaacctCAAA ccgAAAAACA GACTGCCATG CcgtctGA This corresponds to the amino acid sequence <SEQ ID 2100; ORF 650.ng>: g650.pep 1 MSKLKTIALT ASGLSVCPGF LYAQNTΞΞHQ VGLAIMRLNS SILDLPPTKQ 51 YFQSGSLWDE LRQGFRMGEV NPELVRRHES KFIASRSYFD RVVNRSRPYM 101 YHIANEVKKR NMPAEAALLP FIESAFVTKA KSHVGASGLW QFMPATGRHY 151 GLEKTPVYDG RHDVYAATDA ALNYLQYLYG LFGDWPLAFA AYNWGEGNVG 201 RAVNRARDQG LEPTYENLRM PNETRNYVPK LLAVRNIIAT PQSFGMNISD 251 IDNKPYFQAV EPGRPLDNEA IARLAGITQS ELLALNPAFN VPAFIPKNKR 301 KLLLPVASVQ TFQSNYLNAA PDΞLFSWEVY TPAAKTSLSD ISTATGMSIA 351 DIKRLNNLNG NLVNAGRSIL VAKNGKTLHT ASEΞVVSIDI DNTPDTYRSN 401 MPAGTVNVSI ARIQPAAAQT ADITVAPLPQ ETVRTGTRSP CPHYRTRPCD 451 SRSATSNRKT DCHAV* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2101>: m650.seq 1 ATGTCCAAAC TCAAAACCAT CGCTCTGACC GCATCAGGTC TGTCCGTTTG 51 TCCGGGTTTC CTATACGCCC AAAACACCTC ATCACACCAA ATCGGTTTGG 101 CGATTATGCG CTTAAACTCT TCAATACTCG ACCTGCCCCC GACAAAACAA 151 TATTTCCAAT CCGGCAGCCT GTGGGGCGAG CTGCGCCAAG GCTTCCGGAT 201 GGGCGAAGTC AATCCCGAAC TGGTACGCCG CCACGAAAGC AAATTCATCG 251 CAAGCCACAG CTATTTCAAC AGGGTCATCA ACCGGAGTAG ACCCTATATG 301 TACCATATCG CCAACGAAGT CAAAAAACGC AATATGCCCG CCGAAGCCGC 351 CCTGCTTCCC TTCATCGAAA GCGCGTTCGT CACCAAAGCC AAATCACACG 401 TCGGCGCATC AGGATTATGG CAGTTTATGC CCGCTACCGG CAGGCATTAC 451 GGCCTGGAAA AAACACCGGT TTACGACGGC AGGCACGACG TTTACGCCGC 501 CACCGATGCC GCACTCAACT ATCTGCAATA CCTCTATGGA CTGTTCGGCG 551 ACTGGCCGCT TGCCTTTGCC GCCTACAACT GGGGTGAAGG CAACGTCGGA 601 CGCGCCATCA ACCGCGCCCG CGCCCAAGGG CTCGAACCGA CCTACGAAAA 651 CCTGCGTATG CCCAACGAAA CGCGCAACTA TGTCCCCAAG CTGCTCGCCG 701 TGCGCAACAT TATTGCCACT CCCCAATCTT TCGGCATGAA TATCAGCGAC 751 ATAGACAACA AACCCTATTT TCAGGCAGTC GAACCGGATC GTCCGCTCGA 801 CAACGAAGCC ATCGCCCGGC TTGCCGGCAT CACGCAAAGC GAGCTGCTCG 851 CCCTAAACCC CGCATTCAAC GTCCCCGCGT TTATCCCCAA AAGCAAACGC 901 AAACTGCTGC TTCCTGTCGC GTCCGTACAA ACCTTCCAAA GCAACTACCT 951 CAACGCCGCA CCCGACAGCC TGTTTTCATG GGAAGTCTAT ACGCCTGCCG 1001 CCAAAACCAG CCTGTCCGAC ATCTCGACGG CAACCGGCAT GAGCATTGCC 1051 GACATCAAAC GCCTCAACAA CCTGAACGGC AACCTTGTCA ACGCAGGACG 1101 CAGCATCCTT GTCGCCAAGA ACGGCAAAAC CCTTCAGACG GCATCGGAAT 1151 CCGTCGTTTC CATCGACATC GACAATACGC CCGACACCTA CCGTTCCAAT 1201 ATGCCGGCAG GCACGGTGAA CGTCGGCATT GCCCGAATCC GACCCGCCGC 1251 CGCACAGACA GCGGACATTA CCGTCGCACC TTTGCCGCAG AAAACCGTCC 1301 GTACGG.AAC CCGATCCCCT TGTCCGTATT GCCGAACCTG CCCTTGCGAC 1351 AGCCGCAGCG CAACCTCAAA CCGAAAAACA GACCGCCATG CCGTCTGA This corresponds to the amino acid sequence <SEQ ID 2102; ORF 650>: m650.pep 1 MSKLKTIALT ASGLSVCPGF LYAQΝTΞSHQ IGLAIMRLΝS SILDLPPTKQ 51 YFQSGSLWGE LRQGFRMGEV NPELVRRHES KFIASHSYFN RVINRSRPYM 101 YHIANEVKKR NMPAEAALLP FIESAFVTKA KSHVGASGLW QFMPATGRHY 151 GLEKTPVYDG RHDVYAATDA ALNYLQYLYG LFGDWPLAFA AYNWGEGNVG 201 RAINRARAQG LEPTYENLRM PNETRNYVPK LLAVRNIIAT PQSFGMNISD 251 IDNKPYFQAV EPDRPLDNEA IARLAGITQS ELLALNPAFN VPAFIPKSKR 301 KLLLPVASVQ TFQSNYLNAA PDSLFSWEVY TPAAKTSLSD ISTATGMSIA 351 DIKRLNNLNG NLVNAGRSIL VAKNGKTLQT ASESVVΞIDI DNTPDTYRSN 401 MPAGTVNVGI ARIRPAAAQT ADITVAPLPQ KTVRTXTRSP CPYCRTCPCD 451 SRSATSNRKT DRHAV* m650/g650 96.1% identity in 465 aa overlap 10 20 30 40 50 60 m650.pep MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSΞILDLPPTKQYFQSGSLWGE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M I I I I I I I I I I I I I I I I I I I I I I I I I I I I g650 MSKLKTIALTASGLSVCPGFLYAQNTSSHQVGLAIMRLNSSILDLPPTKQYFQSGSLWDE 10 20 30 40 50 60 70 80 90 100 110 120 m650.pep LRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRΞRPYMYHIANEVKKRNMPAEAALLP I I I I I I I I I I I I I I I I I I I I I I I I I : I I Ml I :| I I I I I I I I I I I I I I I I I I I I I I I I I I g650 LRQGFRMGEVNPELVRRHESKFIAΞRSYFDRVVNRSRPYMYHIANEVKKRNMPAEAALLP 70 80 90 100 110 120 130 140 150 160 170 180 m650.pep FIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDGRHDVYAATDAALNYLQYLYG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g650 FIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDGRHDVYAATDAALNYLQYLYG 130 140 150 160 170 180 190 200 210 220 230 240 m650.pep LFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENLRMPNETRNYVPKLLAVRNIIAT I I I I I I I I I I I I I I I I I I I I I l I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g650 LFGDWPLAFAAYNWGEGNVGRAVNRARDQGLEPTYENLRMPNETRNYVPKLLAVRNIIAT 190 200 210 220 230 240 250 260 270 280 290 300 m650.pep PQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAGITQSELLALNPAFNVPAFIPKSKR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I g650 PQSFGMNISDIDNKPYFQAVEPGRPLDNEAIARLAGITQSELLALNPAFNVPAFIPKNKR 250 260 270 280 290 300 310 320 330 340 350 360 m650.pep KLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAAKTSLSDIΞTATGMSIADIKRLNNLNG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g650 KLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAAKTΞLSDISTATGMSIADIKRLNNLNG 310 320 330 340 350 360 370 380 390 400 410 420 m650.pep NLVNAGRSILVAKNGKTLQTASESVVSIDIDNTPDTYRSNMPAGTVNVGIARIRPAAAQT I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I : I I I I I I g650 NLVNAGRSILVAKNGKTLHTAΞESVVSIDIDNTPDTYRSNMPAGTVNVSIARIQPAAAQT 370 380 390 400 410 420 430 440 450 460 m650.pep ADITVAPLPQKTVRTXTRSPCPYCRTCPCDSRSATSNRKTDRHAVX I I I I I I I I I I : I I I I I I I I I I : I I ll ll l l ll l l l l l l l l l l g650 ADITVAPLPQETVRTGTRSPCPHYRTRPCDSRSATSNRKTDCHAVX 430 440 450 460 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2103>: a650. seq 1 ATGTCCAAAC TCAAAACCAT CGCCCTGACC GCGTCAGGTC TGTCCGTTTG 51 TCCGGGTTTC CTATACGCCC AAAACACCTC ATCACACCAA ATCGGTTTGG 101 CGATTATGCG CTTAAACTCT TCAATACTCG ACCTGCCACC GACAAAACAA 151 TATTTCCAAT CCGGCAGCCT GTGGAGCGAG CTGCGCCAAG GCTTCCGGAT 201 GGGCGAAGTC AATCCCGAAC TGGTACGCCG CCACGAAAGC AAATTCATCG 251 CAAGCCACAG CTATTTCAAC AGGGTCATCA ACCGGAGTAG ACCCTATATG 301 TACCATATCG CCAACGAAGT CAAAAAACGC AATATGCCCG CCGAAGCCGC 351 CCTGCTTCCC TTCATCGAAA GCGCGTTCGT CACCAAAGCC AAATCACACG 401 TCGGCGCATC GGGCCTGTGG CAGTTCATGC CCGCTACCGG CAGGCATTAC 451 GGCCTGGAAA AAACACCGGT TTACGACGGC AGGCACGACA TTTACGCCGC 501 CACCGATGCC GCACTCAACT ATCTGCAATA CCTCTATGGA CTGTTCGGCG 551 ACTGGCCGCT CGCCTTTGCC GCCTACAACT GGGGTGAAGG CAACGTCGGA 601 CGCGCCATCA ACCGCGCCCG CGCCCAAGGG CTCGAACCGA CCTACGAAAA 651 CCTGCGTATG CCCAACGAAA CGCGCAACTA TGTTCCCAAG CTGCTCGCCG 701 TGCGCAACAT CATTGCCGCC CCCCAATCTT TCGGCATGAA TATCAGCGAC 751 ATAGACAACA AACCGTATTT TCAGGCAGTC GAACCGGACC GTCCGCTCGA 801 CAACGAAGCC ATCGCCCGGC TTGCCGGCAT CACGCAAAGC GAGCTGCTCG 851 CCCTAAACCC CGCATTCAAC GTCCCCGCGT TCATCCCCAA AAGCAAACGC 901 AAACTGCTGC TTCCTGTCGC GTCCGTACAA ACCTTCCAAA GCAACTACCT 951 CAACGCCGCA CCCGACAGCC TGTTTTCATG GGAAGTCTAT ACGCCTGCCG 1001 CCAAAACCAG CTTGTCCGAC ATCTCGACGG CAACCGGCAT GAGCATTGCC 1051 GACATCAAAC GCCTCAACAA CCTGAACGGC AACCTTGTCA ACGCAGGACG 1101 CAGCATCCTT GTCGCCAAGA ACGGCAAAAC CCTTCAGACG GCATCGGAAT 1151 CCGTCGTTTC CATCGACATC GACAATACGC CCAACACCTA CCGTTCCAAT 1201 ATGCCGGCAG GCACGGTGAA CGTCGGCATT GCCCGAATCC GACCCGCCGC 1251 CGCACAGACA GCGGACATTA CCGTCGCACC TTTGCCGCAG AAAACCGTCC 1301 GTACGG.AAC CCGATCCCCT TGTCCGTATT GCCGAACCTG CCCTTGCGAC 1351 AGCCGCAGCG CAACCTCAAA CCGAAAAACA GACCGCCATG CCGTCTGA This corresponds to the amino acid sequence <SEQ ID 2104; ORF 650. a>: a650.pep 1 MSKLKTIALT ASGLSVCPGF LYAQNTSSHQ IGLAIMRLNS SILDLPPTKQ 51 YFQSGSLWSE LRQGFRMGEV NPELVRRHES KFIASHSYFN RVINRSRPYM 101 YHIANEVKKR NMPAEAALLP FIESAFVTKA KSHVGASGLW QFMPATGRHY 151 GLEKTPVYDG RHDIYAATDA ALNYLQYLYG LFGDWPLAFA AYNWGEGNVG 201 RAINRARAQG LEPTYENLRM PNETRNYVPK LLAVRNIIAA PQSFGMNISD 251 IDNKPYFQAV EPDRPLDNEA IARLAGITQS ELLALNPAFN VPAFIPKSKR 301 KLLLPVASVQ TFQSNYLNAA PDSLFSWEVY TPAAKTSLSD ISTATGMSIA 351 DIKRLNNLNG NLVNAGRSIL VAKNGKTLQT ASESWSIDI DNTPNTYRSN 401 MPAGTVNVGI ARIRPAAAQT ADITVAPLPQ KTVRTXTRSP CPYCRTCPCD 451 SRSATSNRKT DRHAV* m650/a650 99.1% identity in 465 aa overlap 10 20 30 40 50 60 m650. pep MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILDLPPTKQYFQSGSLWGE MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a650 MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILDLPPTKQYFQSGSLWSE 10 20 30 40 50 60 70 80 90 100 110 120 m650.pep LRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYHIANEVKKRNMPAEAALLP MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a650 LRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYHIANEVKKRNMPAEAALLP 70 80 90 100 110 120 130 140 150 160 170 180 m650. pep FIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDGRHDVYAATDAALNYLQYLYG MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a650 FIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDGRHDIYAATDAALNYLQYLYG 130 140 150 160 170 180 190 200 210 220 230 240 m650. pep LFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENLRMPNETRNYVPKLLAVRNIIAT MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a650 LFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENLRMPNETRNYVPKLLAVRNIIAA 190 200 210 220 230 240 250 260 270 280 290 300 m650.pep PQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAGITQSELLALNPAFNVPAFIPKSKR MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a650 PQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAGITQSELLALNPAFNVPAFIPKSKR 250 260 270 280 290 300 310 320 330 340 350 360 m650. pep KLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAAKTSLSDISTATGMSIADIKRLNNLNG MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a650 KLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAAKTSLSDISTATGMSIADIKRLNNLNG 310 320 330 340 350 360 370 380 390 400 410 420 m650.pep NLVNAGRSILVAKNGKTLQTASESWSIDIDNTPDTYRSNMPAGTVNVGIARIRPAAAQT I I II II M II II II II II II I II I I I I I I II II M I I I I I I I II II II II I II II II II I a650 NLVNAGRSILVAKNGKTLQTASESWSIDIDNTPNTYRSNMPAGTVNVGIARIRPAAAQT 370 380 390 400 410 420 430 440 450 460 m650.pep ADITVAPLPQKTVRTXTRSPCPYCRTCPCDSRSATSNRKTDRHAVX II II II I II II II I I I I II II I II I I II II I I II II II I I I I II II a650 ADITVAPLPQKTVRTXTRSPCPYCRTCPCDSRSATSNRKTDRHAVX 430 440 450 460 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2105>: g652. seq 1 ATGATCGAAT TGGACGGTAC TGAAAACAAA GGCAATTTGG GTGCGAATGC 51 GACTTTGGCG GTCTCTATGG CGGTTGCACG CGCCGCTGCC GAAGACTCAG 101 GCCTGCCGCT TTACCGCTAC TTGGGGGGCG CAGGTCCGAT GTCCCTGCCC 151 GTACCGATGA TGAACGTCAT CAACGGCGGC GAACACGCCA ACAACAGCCT 201 GAACATCCAA GAGTTTATGA TTATGCCCGT CGGCGCAAAA TCTTTCCGCG 251 AAGCGTTGCG CTGCGGTGCG GAAATTTTCC ACGCCTTGAA AAAACTGTGC 301 GACAGTAAAG GCTTCCCGAC CACAGTCGGC GACGAAGGCG GTTTCGCCCC 351 CAACCTGAAC AGCCACAAAG AAGCCCTGCA ACTGATGGTC GAAGCGGCCG 401 AAGCCGCCGG CTACAAGGCG GGCGAAGACG TATTATTCGC ATTGGACTGC 451 GCGTCCAGCG AGTTCTACAA AGACGGCAAA TACCACTTGG AAGCCGAAGG 501 CCGCTCCTAC ACCAACGCGG AATTTGCCGA ATACTTGGAA GGCTTGGTTA 551 ACGAATTCCC GATTATTTCC ATTGAAGACG GGATGGACGA AAACGACTGG 601 GAAGGCTGGA AACTGCTGAC CGAAAAATTG GGCAAAAAAG TTCAATTGGT 651 CGGCGACGAC TTGTTCGTAA CCAATCCGAA AATTCTTGCC GAAGGCATCG 701 AAAAAGGCGT AGCAAACGCA TTGCTGGTCA AAGTCAACCA AATCGGTACT 751 TTAAGCGAAA CCCTGAAAGc cgtcgatctg gCAAAATGCA accgctacGc 801 cagCGTGATG AGCCAccgct ccggCGAAAC CGAAGACAGT Accattgccg 851 ACTTGGCAGT CGCCACCAAC TGTATGCAGA TTAAAAccgG TTCTTTGAGc 901 cgTTCCGACC GCATGGCGAA ATACAACCAa ctGCTGCGTA TCGAGGAAGA 951 ATTGGCGGAA GCcgcctACT ACCCCGGCAA AGCCGCATTC TACCAACTGG 1001 GCAAATAA This corresponds to the amino acid sequence <SEQ ID 2106; ORF 652.ng>: g652.pep 1 MIELDGTEΝK GΝLGAΝATLA VSMAVARAAA EDSGLPLYRY LGGAGPMSLP 51 VPMMΝVIΝGG EHAΝΝSLΝIQ EFMIMPVGAK SFREALRCGA EIFHALKKLC 101 DSKGFPTTVG DEGGFAPΝLΝ SHKEALQLMV EAAEAAGYKA GEDVLFALDC 151 ASSEFYKDGK YHLEAEGRSY TΝAEFAEYLE GLVΝEFPIIS IEDGMDEΝDW 201 EGWKLLTEKL GKKVQLVGDD LFVTΝPKILA EGIEKGVAΝA LLVKVΝQIGT 251 LSETLKAVDL AKCΝRYASVM SHRSGETEDS TIADLAVATΝ CMQIKTGSLS 301 RSDRMAKYΝQ LLRIEEELAE AAYYPGKAAF YQLGK* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2107>: 652.seq 1 ATGATCGAAT TGGACGGTAC TGAAAACAAA GGCAATTTGG GTGCGAATGC 51 GACTTTGGCG GTTTCTATGG CGGTTGCACG CGCCGCTGCC GAAGACTCAG 101 GCCTGCCGCT TTACCGCTAC TTGGGCGGCG CAGGCCCGAT GTCCCTGCCC 151 GTACCGATGA TGAACGTCAT CAACGGCGGC GAACACGCCA ACAACAGCCT 201 GAACATCCAA GAGTTTATGA TTATGCCCGT CGGCGCAAAA TCTTTCCGCG 251 AAGCGTTGCG CTGCGGTGCG GAAATTTTCC ACGCCTTGAA AAAACTGTGC 301 GACAGCAAAG GCTTCCCGAC CACAGTCGGC GACGAAGGCG GTTTCGCCCC 351 CAACCTGAAC AGCCACAAAG AAGCCCTGCA ACTGATGGTC GAGGCGACCG 401 AAGCCGCCGG CTACAAAGCG GGCGAAGACG TATTATTCGC ATTGGACTGC 451 GCCTCCAGCG AGTTCTACAA AGACGGCAAA TACCACTTGG AAGCCGAAGG 501 CCGCTCCTAC ACCAACGCGG AATTTGCCGA ATATCTGGAA GGCCTGGTCA 551 ACGAGTTCCC CATCATCTCC ATCGAAGACG GCATGGATGA AAACGACTGG 601 GAAGGCTGGA AACTGCTGAC CGAAAAACTG GGCGGTAGAG TTCAATTGGT 651 TGGCGACGAC TTGTTCGTAA CCAATCCAAA AATCTTGGCC GAAGGCATCG 701 AAAAAGGCGT AGCAAACGCA TTGCTGGTCA AAGTCAATCA AATCGGTACT 751 TTGAGCGAGA CCCTGAAAGC CGTCGACTTA GCCAAACGCA ACCGCTACGC 801 CAGCGTAATG AGCCACCGCT CCGGCGAAAC CGAAGACAGC ACCATTGCCG 851 ACTTGGCAGT CGCCACCAAC TGTATGCAGA TCAAAACCGG TTCTTTGAGC 901 CGTTCCGACC GCATGGCGAA ATACAACCAA CTGCTGCGTA TCGAGGAAGA 951 ATTGGCGGAA GCCGCCGACT ACCCCAGCAA AGCCGCATTC TACCAACTGG 1001 GCAAATAA This corresponds to the amino acid sequence <SEQ ID 2108; ORF 652>: m652.pep 1 MIELDGTEΝK GΝLGAΝATLA VSMAVARAAA EDSGLPLYRY LGGAGPMSLP 51 VPMMΝVIΝGG EHAΝΝSLΝIQ EFMIMPVGAK SFREALRCGA EIFHALKKLC 101 DSKGFPTTVG DEGGFAPNLN SHKEALQLMV EATEAAGYKA GEDVLFALDC 151 ASSEFYKDGK YHLEAEGRSY TNAEFAEYLE GLVNEFPIIS IEDGMDENDW 201 EGWKLLTEKL GGRVQLVGDD LFVTNPKILA EGIEKGVANA LLVKVNQIGT 251 LSETLKAVDL AKRNRYASVM SHRSGETEDS TIADLAVATN CMQIKTGSLS 301 RSDRMAKYNQ LLRIEEELAE AADYPSKAAF YQLGK* m652/g652 98.2% identity in 335 aa overlap 10 20 30 40 50 60 m652.pep MIELDGTENKGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGG 111111111111 ii 1111111111111111111111111111111111111111111111 g652 MIELDGTENKGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGG 10 20 30 40 50 60 70 80 90 100 110 120 m652.pep EHANNSLNIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g652 EHANNSLNIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLN 70 80 90 100 110 120 130 140 150 160 170 180 m652. pep SHKEALQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLE I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g652 SHKEALQLMVEAAEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLE 130 140 150 160 170 180 190 200 210 220 230 240 m652.pep GLVNEFPIIΞIEDGMDENDWEGWKLLTEKLGGRVQLVGDDLFVTNPKILAEGIEKGVANA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I g652 GLVNEFPIISIEDGMDENDWEGWKLLTEKLGKKVQLVGDDLFVTNPKILAEGIEKGVANA 190 200 210 220 230 240 250 260 270 280 290 300 m652.pep LLVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDΞTIADLAVATNCMQIKTGSLΞ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g652 LLVKVNQIGTLΞETLKAVDLAKCNRYAΞVMSHRSGETEDSTIADLAVATNCMQIKTGΞLS 250 260 270 280 290 300 310 320 330 m652.pep RSDRMAKYNQLLRIEEELAEAADYPSKAAFYQLGKX I I II I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I g652 RSDRMAKYNQLLRIEEELAEAAYYPGKAAFYQLGKX 310 320 330 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2109>: a652 .seq 1 ATGATCGAAT TGGACGGTAC TGAAAACAAA GGCAATTTGG GTGCGAATGC 51 GACTTTGGCG GTTTCTATGG CGGTTGCACG CGCCGCTGCC GAAGACTCAG 101 GCCTGCCGCT TTACCGCTAC TTGGGCGGCG CAGGCCCGAT GTCCCTGCCC 151 GTACCGATGA TGAACGTCAT CAACGGCGGC GAACACGCCA ACAACAGCCT 201 GAACATCCAA GAGTTTATGA TTATGCCCGT CGGCGCAAAA TCTTTCCGCG 251 AAGCGTTGCG CTGCGGTGCG GAAATTTTCC ACGCCTTGAA AAAACTGTGC 301 GACAGCAAAG GCTTCCCGAC CACAGTCGGC GACGAAGGCG GTTTCGCCCC 351 CAACCTGAAC AGCCACAAAG AAGCCCTGCA ACTGATGGTC GAGGCGACCG 401 AAGCCGCCGG CTACAAAGCG GGCGAAGACG TATTATTCGC ATTGGACTGC 451 GCGTCCAGCG AGTTCTACAA AGACGGCAAA TACCACTTGG AAGCCGAAGG 501 CCGCTCCTAC ACCAACGCGG AATTTGCCGA ATATCTGGAA GGCCTGGTCA 551 ACGAGTTCCC CATCATCTCC ATCGAAGACG GGATGGATGA AAACGACTGG 601 GAAGGCTGGA AACTGCTGAC CGAAAAACTG GGCGGCAAAG TCCAACTCGT 651 TGGCGACGAC CTCTTCGTTA CCAACCCGAA AATCCTTGCC GAAGGCATTG 701 AAAAAGGCGT GGCAAACGCA CTATTGGTCA AAGTCAACCA AATCGGTACT 751 TTGAGTGAAA CCCTGAAAGC CGTCGACTTA GCCAAACGCA ACCGCTACGC 801 CAGCGTAATG AGCCACCGCT CCGGCGAAAC CGAAGACAGC ACCATTGCCG 851 ACTTGGCAGT CGCCACCAAC TGTATGCAGA TCAAAACCGG TTCTTTGAGC 901 CGTTCCGACC GCATGGCGAA ATACAACCAA CTGCTGCGTA TCGAGGAAGA 951 ATTGGCGGAA GCCGCCGACT ACCCCAGCAA AGCCGCATTC TACCAACTGG 1001 GCAAATAA This corresponds to the amino acid sequence <SEQ ID 2110; ORF 652. a>: a652.pep 1 MIELDGTEΝK GΝLGAΝATLA VSMAVARAAA EDSGLPLYRY LGGAGPMSLP 51 VPMMΝVIΝGG EHAΝΝΞLΝIQ EFMIMPVGAK SFREALRCGA EIFHALKKLC 101 DSKGFPTTVG DEGGFAPΝLΝ SHKEALQLMV EATEAAGYKA GEDVLFALDC 151 ASSEFYKDGK YHLEAEGRSY TNAEFAEYLE GLVNEFPIIS IEDGMDENDW 201 EGWKLLTEKL GGKVQLVGDD LFVTNPKILA EGIEKGVANA LLVKVNQIGT 251 LSETLKAVDL AKRNRYASVM SHRSGETEDS TIADLAVATN CMQIKTGSLS 301 RSDRMAKYNQ LLRIEEELAE AADYPSKAAF YQLGK* m652/a652 99.7% identity in 335 aa overlap 10 20 30 40 50 60 m652 , ■pep MIELDGTENKGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a652 MIELDGTENKGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGG 10 20 30 40 50 60 70 80 90 100 110 120 m652. .pep EHANNΞLNIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLN I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a652 EHANNSLNIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLN 70 80 90 100 110 120 130 140 150 160 170 180 m652. ■pep SHKEALQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a652 SHKEALQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLE 130 140 150 160 170 180 190 200 210 220 230 240 m652. •pep GLVNEFPIISIEDGMDENDWEGWKLLTEKLGGRVQLVGDDLFVTNPKILAEGIEKGVANA I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I a652 GLVNEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANA 190 200 210 220 230 240 250 260 270 280 290 300 m652. .pep LLVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSLS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a652 LLVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSLS 250 260 270 280 290 300 310 320 330 m652. ■pep RSDRMAKYNQLLRIEEELAEAADYPΞKAAFYQLGKX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a652 RSDRMAKYNQLLRIEEELAEAADYPSKAAFYQLGKX 310 320 330 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2111>: g652-l.seq 1 ATGAGCGCAA TCGTTGATAT TTTCGCCCGC GAAATTTTGG ACTCACGCGG 51 CAACCCCACA GTCGAGTGTG ATGTATTGCT CGAATCCGGC GTAATGGGAC 101 GTGCGGCCGT ACCGAGCGGC GCATCCACCG GTCAGAAAGA AGCTTTGGAA 151 CTTCGCGACG GCGACAAATC CCGCTATTCC GGCAAAGGCG TATTGAAGGC 201 CGTCGAACAC GTCAACAACC AAATCGCCCA AGCCCTCATC GGTATCGATG 251 CCAACGAGCA ATCTTATATC GACCAAATCA TGATCGAATT GGACGGTACT 301 GAAAACAAAG GCAATTTGGG TGCGAATGCG ACTTTGGCGG TCTCTATGGC 351 GGTTGCACGC GCCGCTGCCG AAGACTCAGG CCTGCCGCTT TACCGCTACT 401 TGGGGGGCGC AGGTCCGATG TCCCTGCCCG TACCGATGAT GAACGTCATC 451 AACGGCGGCG AACACGCCAA CAACAGCCTG AACATCCAAG AGTTTATGAT 501 TATGCCCGTC GGCGCAAAAT CTTTCCGCGA AGCGTTGCGC TGCGGTGCGG 551 AAATTTTCCA CGCCTTGAAA AAACTGTGCG ACAGTAAAGG CTTCCCGACC 601 ACAGTCGGCG ACGAAGGCGG TTTCGCCCCC AACCTGAACA GCCACAAAGA 651 AGCCCTGCAA CTGATGGTCG AAGCGGCCGA AGCCGCCGGC TACAAGGCGG 701 GCGAAGACGT ATTATTCGCA TTGGACTGCG CGTCCAGCGA GTTCTACAAA 751 GACGGCAAAT ACCACTTGGA AGCCGAAGGC CGCTCCTACA CCAACGCGGA 801 ATTTGCCGAA TACTTGGAAG GCTTGGTTAA CGAATTCCCG ATTATTTCCA 851 TTGAAGACGG GATGGACGAA AACGACTGGG AAGGCTGGAA ACTGCTGACC 901 GAAAAATTGG GCAAAAAAGT TCAATTGGTC GGCGACGACT TGTTCGTAAC 951 CAATCCGAAA ATTCTTGCCG AAGGCATCGA AAAAGGCGTA GCAAACGCAT 1001 TGCTGGTCAA AGTCAACCAA ATCGGTACTT TAAGCGAAAC CCTGAAAGCC 1051 GTCGATCTGG CAAAATGCAA CCGCTACGCC AGCGTGATGA GCCACCGCTC 1101 CGGCGAAACC GAAGACAGTA CCATTGCCGA CTTGGCAGTC GCCACCAACT 1151 GTATGCAGAT TAAAACCGGT TCTTTGAGCC GTTCCGACCG CATGGCGAAA 1201 TACAACCAAC TGCTGCGTAT CGAGGAAGAA TTGGCGGAAG CCGCCTACTA 1251 CCCCGGCAAA GCCGCATTCT ACCAACTGGG CAAATAA This corresponds to the amino acid sequence <SEQ ID 2112; ORF 652-l.ng>: g652-l.pep 1 MSAIVDIFAR EILDSRGNPT VECDVLLESG VMGRAAVPSG ASTGQKEALE 51 LRDGDKSRYS GKGVLKAVEH VNNQIAQALI GIDANEQSYI DQIMIELDGT 101 ENKGNLGANA TLAVΞMAVAR AAAEDSGLPL YRYLGGAGPM ΞLPVPMMNVI 151 NGGEHANNSL NIQEFMIMPV GAKSFREALR CGAEIFHALK KLCDSKGFPT 201 TVGDEGGFAP NLNSHKEALQ LMVEAAEAAG YKAGEDVLFA LDCASSEFYK 251 DGKYHLEAEG RSYTNAEFAE YLEGLVNEFP IIΞIEDGMDE NDWEGWKLLT 301 EKLGKKVQLV GDDLFVTNPK ILAEGIEKGV ANALLVKVNQ IGTLSETLKA 351 VDLAKCNRYA SVMSHRSGET EDSTIADLAV ATNCMQIKTG SLSRSDRMAK 401 YNQLLRIEEE LAEAAYYPGK AAFYQLGK* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2113>: m652-l.seq 1 ATGAGCGCAA TCGTTGATAT TTTCGCCCGC GAAATTTTGG ACTCACGCGG 51 CAACCCCACA GTCGAGTGTG ATGTATTGCT CGAATCCGGC GTAATGGGAC 101 GCGCAGCCGT ACCGAGCGGC GCGTCCACCG GTCAAAAAGA GGCTTTGGAA 151 CTTCGCGACG GCGACAAATC CCGTTATTCG GGCAAGGGCG TATTGAAGGC 201 GGTCGAACAC GTCAACAACC AAATCGCCCA AGCCCTCATT GGTATCGATG 251 CCAACGAGCA ATCTTATATC GACCAAATCA TGATCGAATT GGACGGTACT 301 GAAAACAAAG GCAATTTGGG TGCGAATGCG ACTTTGGCGG TTTCTATGGC 351 GGTTGCACGC GCCGCTGCCG AAGACTCAGG CCTGCCGCTT TACCGCTACT 401 TGGGCGGCGC AGGCCCGATG TCCCTGCCCG TACCGATGAT GAACGTCATC 451 AACGGCGGCG AACACGCCAA CAACAGCCTG AACATCCAAG AGTTTATGAT 501 TATGCCCGTC GGCGCAAAAT CTTTCCGCGA AGCGTTGCGC TGCGGTGCGG 551 AAATTTTCCA CGCCTTGAAA AAACTGTGCG ACAGCAAAGG CTTCCCGACC 601 ACAGTCGGCG ACGAAGGCGG TTTCGCCCCC AACCTGAACA GCCACAAAGA 651 AGCCCTGCAA CTGATGGTCG AGGCGACCGA AGCCGCCGGC TACAAAGCGG 701 GCGAAGACGT ATTATTCGCA TTGGACTGCG CCTCCAGCGA GTTCTACAAA 751 GACGGCAAAT ACCACTTGGA AGCCGAAGGC CGCTCCTACA CCAACGCGGA 801 ATTTGCCGAA TATCTGGAAG GCCTGGTCAA CGAGTTCCCC ATCATCTCCA 851 TCGAAGACGG CATGGATGAA AACGACTGGG AAGGCTGGAA ACTGCTGACC 901 GAAAAACTGG GCGGTAGAGT TCAATTGGTT GGCGACGACT TGTTCGTAAC 951 CAATCCAAAA ATCTTGGCCG AAGGCATCGA AAAAGGCGTA GCAAACGCAT 1001 TGCTGGTCAA AGTCAATCAA ATCGGTACTT TGAGCGAGAC CCTGAAAGCC 1051 GTCGACTTAG CCAAACGCAA CCGCTACGCC AGCGTAATGA GCCACCGCTC 1101 CGGCGAAACC GAAGACAGCA CCATTGCCGA CTTGGCAGTC GCCACCAACT 1151 GTATGCAGAT CAAAACCGGT TCTTTGAGCC GTTCCGACCG CATGGCGAAA 1201 TACAACCAAC TGCTGCGTAT CGAGGAAGAA TTGGCGGAAG CCGCCGACTA 1251 CCCCAGCAAA GCCGCATTCT ACCAACTGGG CAAATAA This corresponds to the amino acid sequence <SEQ ID 2114; ORF 652- 1>: m652-l.pep 1 MSAIVDIFAR EILDSRGNPT VECDVLLESG VMGRAAVPSG ASTGQKEALE 51 LRDGDKSRYS GKGVLKAVEH VNNQIAQALI GIDANEQSYI DQIMIELDGT 101 ENKGNLGANA TLAVSMAVAR AAAEDSGLPL YRYLGGAGPM SLPVPMMNVI 151 NGGEHANNSL NIQEFMIMPV GAKSFREALR CGAEIFHALK KLCDSKGFPT 201 TVGDEGGFAP NLNSHKEALQ LMVEATEAAG YKAGEDVLFA LDCASSEFYK 251 DGKYHLEAEG RSYTNAEFAE YLEGLVNEFP IISIEDGMDE NDWEGWKLLT 301 EKLGGRVQLV GDDLFVTNPK ILAEGIEKGV ANALLVKVNQ IGTLSETLKA 351 VDLAKRNRYA SVMSHRSGET EDSTIADLAV ATNCMQIKTG SLSRSDRMAK 401 YNQLLRIEEE LAEAADYPSK AAFYQLGK* m652-l/g652-l 98.6% identity in 428 aa overlap 10 20 30 40 50 60 m652-l MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTGQKEALELRDGDKSRYS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g652-l MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTGQKEALELRDGDKSRYS 10 20 30 40 50 60 70 80 90 100 110 120 m652-l GKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTENKGNLGANATLAVSMAVAR I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I II g652-l GKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTENKGNLGANATLAVSMAVAR 70 80 90 100 110 120 130 140 150 160 170 180 m652-l AAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSLNIQEFMIMPVGAKSFREALR I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I II g652-1 AAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSLNIQEFMIMPVGAKSFREALR 130 140 150 160 170 180 190 200 210 220 230 240 m652-l CGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEALQLMVEATEAAGYKAGEDVLFA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I g652-l CGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEALQLMVEAAEAAGYKAGEDVLFA 190 200 210 220 230 240 250 260 270 280 290 300 m652-l LDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLEGLVNEFPIISIEDGMDENDWEGWKLLT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g652-l DCASSEFYKDGKYHLEAEGRΞYTNAEFAEYLEGLVNEFPIISIEDGMDENDWEGWKLLT 250 260 270 280 290 300 310 320 330 340 350 360 m652-l EKLGGRVQLVGDDLFVTNPKILAEGIEKGVANALLVKVNQIGTLSETLKAVDLAKRNRYA llll : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I llll g652-l EKLGKKVQLVGDDLFVTNPKILAEGIEKGVANALLVKVNQIGTLSETLKAVDLAKCNRYA 310 320 330 340 350 360 370 380 390 400 410 420 m652-l SVMSHRSGETEDSTIADLAVATNCMQIKTGΞLSRSDRMAKYNQLLRIEEELAEAADYPSK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I MM g652-l SVMSHRSGETEDSTIADLAVATNCMQIKTGSLSRSDRMAKYNQLLRIEEELAEAAYYPGK 370 380 390 400 410 420 429 m652-l AAFYQLGKX lllllllll g652-l AAFYQLGKX The following partial DNA sequence was identified in N meningitidis <SEQ ID 2115>: a652-l.seq 1 ATGAGCGCAA TCGTTGATAT TTTCGCCCGC GAAATTTTGG ACTCACGCGG 51 CAACCCCACA GTCGAGTGTG ATGTATTGCT CGAATCCGGC GTAATGGGAC 101 GCGCAGCCGT ACCGAGCGGC GCGTCCACCG GTCAAAAAGA GGCTTTGGAA 151 CTTCGCGACG GCGACAAATC CCGTTATTCG GGCAAGGGCG TATTGAAGGC 201 GGTCGAACAC GTCAACAACC AAATCGCCCA AGCCCTCATT GGTATCGATG 251 CCAACGAGCA ATCTTATATC GACCAAATCA TGATCGAATT GGACGGTACT 301 GAAAACAAAG GCAATTTGGG TGCGAATGCG ACTTTGGCGG TTTCTATGGC 351 GGTTGCACGC GCCGCTGCCG AAGACTCAGG CCTGCCGCTT TACCGCTACT 401 TGGGCGGCGC AGGCCCGATG TCCCTGCCCG TACCGATGAT GAACGTCATC 451 AACGGCGGCG AACACGCCAA CAACAGCCTG AACATCCAAG AGTTTATGAT 501 TATGCCCGTC GGCGCAAAAT CTTTCCGCGA AGCGTTGCGC TGCGGTGCGG 551 AAATTTTCCA CGCCTTGAAA AAACTGTGCG ACAGCAAAGG CTTCCCGACC 601 ACAGTCGGCG ACGAAGGCGG TTTCGCCCCC AACCTGAACA GCCACAAAGA 651 AGCCCTGCAA CTGATGGTCG AGGCGACCGA AGCCGCCGGC TACAAAGCGG 701 GCGAAGACGT ATTATTCGCA TTGGACTGCG CGTCCAGCGA GTTCTACAAA 751 GACGGCAAAT ACCACTTGGA AGCCGAAGGC CGCTCCTACA CCAACGCGGA 801 ATTTGCCGAA TATCTGGAAG GCCTGGTCAA CGAGTTCCCC ATCATCTCCA 851 TCGAAGACGG GATGGATGAA AACGACTGGG AAGGCTGGAA ACTGCTGACC 901 GAAAAACTGG GCGGCAAAGT CCAACTCGTT GGCGACGACC TCTTCGTTAC 951 CAACCCGAAA ATCCTTGCCG AAGGCATTGA AAAAGGCGTG GCAAACGCAC 1001 TATTGGTCAA AGTCAACCAA ATCGGTACTT TGAGTGAAAC CCTGAAAGCC 1051 GTCGACTTAG CCAAACGCAA CCGCTACGCC AGCGTAATGA GCCACCGCTC 1101 CGGCGAAACC GAAGACAGCA CCATTGCCGA CTTGGCAGTC GCCACCAACT 1151 GTATGCAGAT CAAAACCGGT TCTTTGAGCC GTTCCGACCG CATGGCGAAA 1201 TACAACCAAC TGCTGCGTAT CGAGGAAGAA TTGGCGGAAG CCGCCGACTA 1251 CCCCAGCAAA GCCGCATTCT ACCAACTGGG CAAATAA This corresponds to the amino acid sequence <SEQ ID 2116; ORF 652-l.a>: a652-l.pep 1 MSAIVDIFAR EILDSRGNPT VECDVLLESG VMGRAAVPSG ASTGQKEALE 51 LRDGDKSRYS GKGVLKAVEH VNNQIAQALI GIDANEQSYI DQIMIELDGT 101 ENKGNLGANA TLAVSMAVAR AAAEDSGLPL YRYLGGAGPM ΞLPVPMMNVI 151 NGGEHANNSL NIQEFMIMPV GAKSFREALR CGAEIFHALK KLCDSKGFPT 201 TVGDEGGFAP NLNSHKEALQ LMVEATEAAG YKAGEDVLFA LDCASSEFYK 251 DGKYHLEAEG RSYTNAEFAE YLEGLVNEFP IISIEDGMDE NDWEGWKLLT 301 EKLGGKVQLV GDDLFVTNPK ILAEGIEKGV ANALLVKVNQ IGTLSETLKA 351 VDLAKRNRYA SVMSHRSGET EDSTIADLAV ATNCMQIKTG SLSRSDRMAK 401 YNQLLRIEEE LAEAADYPSK AAFYQLGK* m652-l/a652-l 99.8% identity in 428 aa overlap 10 20 30 40 50 60 m652-l MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTGQKEALELRDGDKSRYS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a652-l MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTGQKEALELRDGDKSRYS 10 20 30 40 50 60 70 80 90 100 110 120 m652-l GKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTENKGNLGANATLAVΞMAVAR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a652-l GKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTENKGNLGANATLAVSMAVAR 70 80 90 100 110 120 130 140 150 160 170 180 m652-l AAAEDSGLPLYRYLGGAGPMΞLPVPMMNVINGGEHANNSLNIQEFMIMPVGAKSFREALR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a652-l AAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSLNIQEFMIMPVGAKSFREALR 130 140 150 160 170 180 190 200 210 220 230 240 m652-l CGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEALQLMVEATEAAGYKAGEDVLFA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a652-l CGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEALQLMVEATEAAGYKAGEDVLFA 190 200 210 220 230 240 250 260 270 280 290 300 m652-l LDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLEGLVNEFPIISIEDGMDENDWEGWKLLT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II a652-l LDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLEGLVNEFPIISIEDGMDENDWEGWKLLT 250 260 270 280 290 300 310 320 330 340 350 360 m652-l EKLGGRVQLVGDDLFVTNPKILAEGIEKGVANALLVKVNQIGTLSETLKAVDLAKRNRYA I I I I l I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a652-l EKLGGKVQLVGDDLFVTNPKILAEGIEKGVANALLVKVNQIGTLSETLKAVDLAKRNRYA 310 320 330 340 350 360 370 380 390 400 410 420 m652- -1 SVMSHRSGETEDSTIADLAVATNCMQIKTGΞLSRSDRMAKYNQLLRIEEELAEAADYPΞK I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a652- -1 SVMSHRSGETEDΞTIADLAVATNCMQIKTGSLSRSDRMAKYNQLLRIEEELAEAADYPSK 370 380 390 400 410 420 429 m652- -1 AAFYQLGKX lllllllll a652- -1 AAFYQLGKX The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2117>: g653. seq 1 ATGGCGGcgg aaccgatgcg gAtgccggag gtaAcgtaCG GTTTTTCCGG 51 ATCGTTCGGG ATGGCGTTTT TGTtgacggT GATGTGCGCt ttgcccaAAG 101 CGGCTtcggc ggctttgcCg gtgaTTTTCA TCGGTTGCAG GtcgacgaGG 151 AAaacgTGGC TTTCGGTGCG GCCGGAAacg atgcgCaaac cgCGTttaac 201 caactcttcc gcCATGACGG CAGCATTGAT TTTCACTTGT TTTGCGTATT 251 GTTTGAactC GGGTTGcaac gcttctTTAA acgctACGGC TttgGCGGCG 301 ATAACGTgca tcaACGGAcc gCCTTGCAGG CTTGGGAAGA TGGAAGAGTT 351 CAGCGCTTTT TCGTGGGTAT TGTCACGGCA CAAAATCACA CCGCCGCGAG 401 GGCCGCGTAG GGTTTTGTGG GTGGTAGTGg ttACgaaGTc GCAGAatggc 451 ACGGGgttag gatattcgcc gccGGCAACC AgtccgGCAT Ag This corresponds to the amino acid sequence <SEQ ID 2118; ORF 653. ng >: g653.pep 1 MAAEPMRMPE VTYGFSGSFG MAFLLTVMCA LPKAASAALP VIFIGCRSTR 51 KTWLSVRPET MRKPRLTΝSS AMTAALIFTC FAYCLΝSGCΝ ASLΝATALAA 101 ITCIΝGPPCR LGKMEEFSAF ΞWVLSRHKIT PPRGPRRVLW VVVVTKSQΝG 151 TGLGYΞPPAT SPA* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2119>: m653. seq 1 ATGGCAGCGG AGCCGATGCG GATGCCGGAG GTAACGAAGG GTTTTTCCGG 51 ATCGTTCGGA ATGGCGTTTT TGTTGACGGT GATGTGCGCT TTGCCCAAAG 101 CGGCTTCGGC GGCTTTGCCG GTAATTTTCA TCGGTTGCAG GTCAACGAGG 151 AAAACGTGGC TTTCGGTGCG GCCGGAAACG ATGCGCAAAC CGCGTTTAAC 201 CAACTCTTCC GCCATGGCGG CTGCATTGAT TTTCACTTGT TTTGCGTATT 251 GTTTGAACTC GGGTTGCAAT GCTTCTTTAA ACGCCACGGC TTTGGCGGCG 301 ATAACGTGCA TCAGCGGACC GCCTTGCAGG CTTGGGAAGA TGGAAGAGTT 351 CAACGCTTTT TCGTGGGTAT TGTCGCGGCA CAAAATTACG CCGCCGCGAG 401 GACCGCGCAG GGTTTTGTGG GTGGTGGTGG TCACGAAGTC GCAGAACGGC 451 ACCGGGTTGG GATATTCGCC GCCGGCAACC AGACCGGCAT AG This corresponds to the amino acid sequence <SEQ ID 2120; OI 653 .pep 1 MAAEPMRMPE VTKGFSGΞFG MAFLLTVMCA LPKAASAALP VIFIGCRSTR 51 KTWLSVRPET MRKPRLTNSS AMAAALIFTC FAYCLNSGCN ASLNATALAA 101 ITCISGPPCR LGKMEEFNAF ΞWVLΞRHKIT PPRGPRRVLW VVVVTKSQNG 151 TGLGYSPPAT RPA* m653/g653 96.9% identity in 163 aa overlap 10 20 30 40 50 60 m653.pep MAAEPMRMPEVTKGFSGSFGMAFLLTVMCALPKAASAALPVIFIGCRSTRKTWLΞVRPET I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g653 MAAEPMRMPEVTYGFSGSFGMAFLLTVMCALPKAASAALPVIFIGCRSTRKTWLSVRPET 10 20 30 40 50 60 70 80 90 100 110 120 m653.pep MRKPRLTNSSAMAAALIFTCFAYCLNSGCNASLNATALAAITCISGPPCRLGKMEEFNAF I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I : I I g653 MRKPRLTNSSAMTAALIFTCFAYCLNΞGCNASLNATALAAITCINGPPCRLGKMEEFΞAF 70 80 90 100 110 120 130 140 150 160 m653.pep ΞWVLSRHKITPPRGPRRVLWVVVVTKSQNGTGLGYSPPATRPAX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I III g653 SWVLSRHKITPPRGPRRVLWVVVVTKSQNGTGLGYSPPATSPAX 130 140 150 160 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2121 > • a653. seq 1 ATGGCGGCGG AACCGATGCG GATGCCGGAG GTAACGAAGG GTTTTTCCGG 51 ATCATTCGGG ATGGCGTTTT TGTTGACAGT GATGTGCGCT TTGCCCAAAG 101 CAGCTTCGGC GGCTTTGCCG GTAATTTTCA TCGGTTGCAG GTCAACGAGG 151 AAAACGTGGC TTTCGGTGCG GCCGGAAACG ATGCGCAAAC CGCGTTTAAC 201 CAACTCTTCC GCCATGGCGG CTGCATTGAT TTTCACTTGT TTTGCGTATT 251 GTTTGAACTC GGGTTGCAAT GCTTCTTTAA ACGCCACGGC TTTGGCGGCG 301 ATAACGTGCA TCAGCGGGCC ACCTTGCAGG CTTGGGAAGA TGGAAGAGTT 351 CAACGCTTTT TCGTGGGTAT TGTCGCGGCA CAAAATTACG CCGCCGCGAG 401 GACCGCGCAG GGTTTTGTGG GTGGTGGTGG TAACGAAGTC GCAGAACGGC 451 ACGGGATTGG GATATTCGCC GCCGGCAACC AGACCGGCAT AG This corresponds to the amino acid sequence <SEQ ID 2122; ORF 653.a>: a653.pep 1 MAAEPMRMPE VTKGFSGSFG MAFLLTVMCA LPKAASAALP VIFIGCRSTR 51 KTWLSVRPET MRKPRLTNSS AMAAALIFTC FAYCLNSGCN ASLNATALAA 101 ITCISGPPCR LGKMEEFNAF SWVLΞRHKIT PPRGPRRVLW VVVVTKSQNG 151 TGLGYSPPAT RPA* m653/a653 100.0% identity in 163 aa overlap 10 20 30 40 50 60 m653.pep MAAEPMRMPEVTKGFSGSFGMAFLLTVMCALPKAAΞAALPVIFIGCRSTRKTWLSVRPET I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a653 MAAEPMRMPEVTKGFSGSFGMAFLLTVMCALPKAASAALPVIFIGCRSTRKTWLSVRPET 10 20 30 40 50 60 70 80 90 100 110 120 m653.pep MRKPRLTNΞΞAMAAALIFTCFAYCLNΞGCNASLNATALAAITCISGPPCRLGKMEEFNAF I I II I I I I I I I I I I I I I I II I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I a653 MRKPRLTNΞSAMAAALIFTCFAYCLNSGCNASLNATALAAITCISGPPCRLGKMEEFNAF 70 80 90 100 110 120 130 140 150 160 m653.pep SWVLSRHKITPPRGPRRVLWVVVVTKSQNGTGLGYSPPATRPAX I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I II I I I I I I I I I I I a653 SWVLΞRHKITPPRGPRRVLWVVVVTKSQNGTGLGYΞPPATRPAX 130 140 150 160 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2123>: g656. seq 1 ATGCCGCGTT TCTCCGGTTC GATTTCTTCG ATGATTTCCA TCGCGCGGAC 51 TTTtggcGCG CCGGAGAGTG TGCcggcagg gAAGGTGGCG GCGAGGATGT 101 CCATATTGGT AACGCCCTCT TTCAAACAGc ctTCGACGTT GGAAACGATG 151 TGCATCACAT GGGAGTATTT TTCAATCACC ATTTTGTCGG TGACTTTGAC 201 TTCGCCTGTT TTGCTGATGC GTCCGACATC GTTGCGCCCC AAATCGATAA 251 GCATAACGTG TTCGGCgatt TCTTTGGCGT CGCTTAACAA ATCTTGTTCG 301 TTGGCAAGGT CTTCGGCGGG GGTTTTGCCG CGCAGGCGCG TGCCGGCGAT 351 GGGGCGGACG ATGACGTcat CGCGTTCGCG GCGGACGAGG ATTTCGGGCG 401 AGGAACCGAC GATGTGGAAA TCGCCGAAAT CGTAG This corresponds to the amino acid sequence <SEQ ID 2124; ORF 656.ng>: g656.pep 1 MPRFSGSISS MISIARTFGA PESVPAGKVA ARMSILVTPS FKQPSTLETM 51 CITWEYFSIT ILSVTLTSPV LLMRPTSLRP KSISITCSAI SLASLΝKSCS 101 LARSSAGVLP RRRVPAMGRT MTSSRSRRTR ISGEEPTMWK SPKS* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2125>: m656. seq 1 ATGCCGCGTT TGCTCGGTTC GACTTCTTCG ATGATTTCCA TGGCGCGGAC 51 TTTGGGTGCG CCGGAGAGTG TGCCGGCAGG GAAGGTAGCG GCGAGGATGT 101 CCATGTTGGT CATGCCGTCT TTCAGACGGC CTTCGACGTT GGAAACGATG 151 TGCATTACAT GGGAGTATTT TTCAATCACC ATTTTGTCGG TAACTTTGAC 201 TTCGCCGGTT TTACTGATGC GGCCGACGTC GTTGCGTCCT AAGTCAATCA 251 ACATGACGTG TTCGGCGATT TCTTTGGCAT CGCTTAACAA ATCTTGTTCG 301 TTGGCAAGGT CTTCGGCGGG GGTTTTGCCG CGCAGGCGCG TGCCGGCGAT 351 GGGGCGGACG ATAACGTCGT TGCGTTCGCG TCGGACGAGG ATTTCGGGCG 401 AGGAGCCGAC GATGTGGAAA TCGCCGAAAT CGTAG This corresponds to the amino acid sequence <SEQ ID 2126; ORF 656>: m656.pep 1 MPRLLGSTSS MISMARTLGA PESVPAGKVA ARMSMLVMPS FRRPSTLETM 51 CITWEYFSIT ILSVTLTSPV LLMRPTSLRP KSIΝMTCSAI SLASLΝKSCS 101 LARSSAGVLP RRRVPAMGRT ITSLRSRRTR ISGEEPTMWK SPKS* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m656/g656 91.0% identity in 144 aa overlap 10 20 30 40 50 60 m656.pep MPRLLGSTSSMISMARTLGAPESVPAGKVAARMSMLVMPSFRRPSTLETMCITWEYFSIT MM II II II I M I M II II II I II II II II M II II I : M I I I II II II II I II II g656 MPRFSGSISSMISIARTFGAPESVPAGKVAARMSILVTPSFKQPSTLETMCITWEYFSIT 10 20 30 40 50 60 70 80 90 100 110 120 m656. pep ILSVTLTSPVLLMRPTSLRPKSIΝMTCSAISLASLΝKSCSLARSSAGVLPRRRVPAMGRT MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g656 ILSVTLTSPVLLMRPTSLRPKSISITCSAISLASLΝKSCSLARSSAGVLPRRRVPAMGRT 70 80 90 100 110 120 130 140 m656.pep ITSLRSRRTRISGEEPTMWKSPKSX Ml I I II II I I II I I II I II I II I g656 MTSSRSRRTRISGEEPTMWKSPKSX 130 140 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2127>: a656. seq 1 ATGCCGCGTT TGCTCGGTTC GACTTCTTCG ATGATTTCCA TGGCGCGGAC 51 TTTGGGTGCG CCGGAGAGTG TGCCGGCAGG GAAGGTAGCG GCGAGGATGT 101 CCATGTTGGT CATGCCGTCT TTCAGACGGC CTTCGACGTT GGAAACGATG 151 TGCATTACAT GGGAGTATTT TTCAATCACC ATTTTGTCGG TAACTTTGAC 201 TTCGCCGGTT TTACTGATGC GGCCGACGTC GTTGCGTCCT AAGTCAATCA 251 ACATGACGTG TTCGGCGATT TCTTTGGCAT CGCTTAACAA ATCTTGTTCG 301 TTGGCAAGGT CTTCGGCGGG GGTTTTGCCG CGCAGGCGCG TGCCGGCGAT 351 GGGGCGGACG ATGACATCGT CGCGTTCGCG GCGGACGAGG ATTTCGGGCG 401 AGGAGCCGAC GATGTGGAAA TCGCCGAAAT CGTAG This corresponds to the amino acid sequence <SEQ ID 2128; ORF 656. a>: a656.pep 1 MPRLLGSTSS MISMARTLGA PESVPAGKVA ARMSMLVMPS FRRPSTLETM 51 CITWEYFSIT ILSVTLTSPV LLMRPTSLRP KΞIΝMTCΞAI SLASLΝKSCS 101 LARSSAGVLP RRRVPAMGRT MTSSRSRRTR ISGEEPTMWK SPKS* m656/a656 98.6% identity in 144 aa overlap 10 20 30 40 50 60 m656.pep MPRLLGSTSSMIΞMARTLGAPESVPAGKVAARMSMLVMPΞFRRPSTLETMCITWEYFSIT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a656. MPRLLGSTSSMISMARTLGAPESVPAGKVAARMSMLVMPSFRRPSTLETMCITWEYFSIT 10 20 30 40 50 60 70 80 90 100 110 120 m656.pep ILSVTLTSPVLLMRPTSLRPKSIΝMTCSAISLASLΝKSCSLARSSAGVLPRRRVPAMGRT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a656 ILSVTLTSPVLLMRPTΞLRPKΞIΝMTCSAISLASLΝKSCΞLARΞΞAGVLPRRRVPAMGRT 70 80 90 100 110 120 130 140 m656.pep ITSLRSRRTRISGEEPTMWKSPKSX l I I I I I I I I I I I I I I I I I I I I I a656 MTSSRSRRTRISGEEPTMWKSPKSX 130 140 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 2129>: g657. seq 1 ATGAACACAC CCCCCATCCT TCCTCCCGCC ATGCTCGGCA TCCTCGGCGG 51 CGGACAATTa ggcagAATGT TTGCCGTTGC CGCTAAAACC ATGGGCTACA 101 AAGTAACCGT TCTCGATCCC GACCCGAATG CGCCGGCGGC GGAATTTGCC 151 GACCGCCATT TGTGCGCGCC GTTTGACGAC CGGGCCGCGT TGGACGAATT 201 GGCAAAATGC GCGGCGGTta cgACCGAATT TGAAAacgtc aaTGCCGACG 251 CGATGCGCTC TCTGGCAAAG CATACCAACG TTTCCCCCAG CGGCGACTGC 301 GTGTCCATTG CACAAAACCG CATTCAGGAA AAAGCGTGGA TACGCAAAGC 351 AGGCTTGCAA ACCGCGCCGT ATCAGGCGGT TTGCAAGGCC GAAGACATTA 401 CTGAAGCAAG CGCGCAATTT TTGCCCGGCA TCCTGAAAAC GGCTACGTTG 451 GGCTACGACG GCAAAGGTCA AATCCGCGTC AAAACGTTGG ACGAACTCAA 501 AGCCGCGTTT GCCGAACACG GCGGCGTGGA TTGCGTTTTG GAAAAAATGG 551 TGGACTTGCG CGGCGAGATT TCCGTGATCG TATGCCGTCT GAACGATGAA 601 AACGTGCAAA CCTTCGACCC CGCCGAAAAC ATCCACGAAA ACGGCATCTT 651 GGCTTattcC ATCGTCcccg CGCGGCTGAG TGCCGACGTG CAGCAACAGG 701 CGCGGCAGAC GGCGCAACgc tTGGCGGACG AATTGGATTA TGTCGGCgta 751 TTGGCGGTAG AAATGTTTGT TGTCGGCGAC ACACATGAAT TGCTCGTCAA 801 TGAAACCGCC CCGCGCACGC ACAATTCCGG CCACCATACG ATAGATGCCT 851 GCGCCGCAGA CCAGTTCCAA CAGCAGGTAC GCATTATGTG CAAcctGCCG 901 cccGccgACA CCAAATTATT aTCCCCttgC TGTATGGCGA ATATTTTGGg 951 CGACGTTTGG CAGGAAGATG GCGGCGAACC GGATTGGCTG CCGTTGCAAA 1001 GCCGGCCGAA TGCACACCTG CACCTATACG GAAAAAAAAC CGCACAGAAA 1051 GGTCGGAAAA TGGGACACTT TaccgTTTTG ACCACCGATT CGGACaccgC 1101 ATTTCAAGAA GCAAAAAAAC TGCATCAGTC CCTATAA This corresponds to the amino acid sequence <SEQ ID 2130; ORF 657.ng>: MNTPPILPPA MLGILGGGQL GRMFAVAAKT MGYKVTVLDP DPNAPAAEFA DRHLCAPFDD RAALDELAKC AAVTTEFENV NADAMRSLAK HTNVSPSGDC VSIAQNRIQE KAWIRKAGLQ TAPYQAVCKA EDITEASAQF LPGILKTATL GYDGKGQIRV KTLDELKAAF AEHGGVDCVL EKMVDLRGEI SVIVCRLNDE NVQTFDPAEN IHENGILAYS IVPARLSADV QQQARQTAQR LADELDYVGV 251 LAVEMFVVGD THELLVNETA PRTHNSGHHT IDACAADQFQ QQVRIMCNLP 301 PADTKLLSPC CMANILGDVW QEDGGEPDWL PLQSRPNAHL HLYGKKTAQK 351 GRKMGHFTVL TTDSDTAFQE AKKLHQSL* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2131>: m657. seq 1 ATGAAAAACA TATCTCTTTC TCCGCCCGCC ATGCTTGGCA TCCTCGGCGG 51 CGGACAATTA GGCAGAATGT TTACCGTTGC CGCCAAAACC ATGGGCTACA 101 AAGTAACCGT TCTCGACCCC GATCCGGACG CGCCGGCAGC AGAATTTGCC 151 GACCGCCATT TGTGCGCGCC GTTTAACGAC CAAGCTGCTT TGGACGAATT 201 GGCAAAATGC GCGGCGGTGA CCACTGAATT TGAAAACGTC AATGCCGATG 251 CGATGCGCTT TTTGGCAAAA CATACCAATG TTTCCCCTAG CGGCGATTGT 301 GTGGCGATTG CACAAAACCG CATTCAGGAA AAGGCATGGA TACGCAAAGC 351 GGGATTGCAA ACCGCGCCGT ATCAAGTGGT TTGTAAGGCT GAAGACATCA 401 CTGAAGCAAG CGCGCAATTT TTGCCCGGCA TCCTGAAAAC GGCTACGTTG 451 GGCTACGACG GCAAAGGTCA AATCCGCGTA AAAACATTGG ATGAACTCAA 501 AGCCGCGTTT GCCGAACACG GCGGCGTGGA TTGCGTTTTG GAAAAAATGG 551 TGGATTTGCG CAGTGAAATT TCCGTAATCG TATGCCGTTT GAACAATGAC 601 AACGTGCAAA CTTTCGACCC TGCCGAAAAC ATCCACGAAA ACGGCATCTT 651 GGCTTATTCC ATCGTCCCCG CGCGACTGAG TGCCGACGTG CAGCAACAGG 701 CGCGGCAGAT GGCGCAACGC TTGGCGGACG AATTGGATTA TGTCGGCGTA 751 TTGGCGGTAG AAATGTTTGT TGTCGGTGAC ACGCATGAAT TGGTCGTCAA 801 CGAAATCGCC CCGCGCCCGC ACAATTCCGG ACACCATACG ATAGATGCCT 851 GCGCAGCAGA CCAGTTCCAG CAGCAGGTAC GCATTATGTG CAACCTGCCG 901 CCTGCCGATA CCAAATTACT GAGTTCTTGC TGTATGGCAA ATATTTTGGG 951 CGACGTTTGG CAGGAAGACG GCGGCGAACC GGATTGGCTG CCCTTGCAAA 1001 GCCATCCGAA TGCACACCTG CACCTTTACG GCAAAAAAAC CGCGCACAAA 1051 GGGCGGAAAA TGGGACACTT TACCGTTTTA ACCACCGATT CGGACACCGC 1101 ATTTCAAGAA GCAAAAAAAC TGCATCAGTC CCTATAA This corresponds to the amino acid sequence <SEQ ID 2132; ORF 657>: m657.pep 1 MKΝISLSPPA MLGILGGGQL GRMFTVAAKT MGYKVTVLDP DPDAPAAEFA 51 DRHLCAPFΝD QAALDELAKC AAVTTEFENV NADAMRFLAK HTNVSPSGDC 101 VAIAQNRIQE KAWIRKAGLQ TAPYQWCKA EDITEASAQF LPGILKTATL 151 GYDGKGQIRV KTLDELKAAF AEHGGVDCVL EKMVDLRSEI SVIVCRLNND 201 NVQTFDPAEN IHENGILAYS IVPARLSADV QQQARQMAQR LADELDYVGV 251 LAVEMFVVGD THEL VNEIA PRPHNSGHHT IDACAADQFQ QQVRIMCNLP 301 PADTKLLSSC CMANILGDVW QEDGGEPDWL PLQSHPNAHL HLYGKKTAHK 351 GRKMGHFTVL TTDSDTAFQE AKKLHQSL* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m657/g657 93.9% identity in 378 aa overlap 10 20 30 40 50 60 m657.pep MKΝISLΞPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPDPDAPAAEFADRHLCAPFΝD M: : II II I II II I II II I I I : II II I II I I II I II II I : II I II II I II II I II : I g657 MΝTPPILPPAMLGILGGGQLGRMFAVAAKTMGYKVTVLDPDPΝAPAAEFADRHLCAPFDD 10 20 30 40 50 60 70 80 90 100 110 120 m657 . pep QAALDELAKCAAVTTEFEΝVΝADAMRFLAKHTΝVSPSGDCVAIAQΝRIQEKAWIRKAGLQ : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I g657 RAALDELAKCAAVTTEFEΝVΝADAMRSLAKHTΝVSPSGDCVSIAQΝRIQEKAWIRKAGLQ 70 80 90 100 110 120 130 140 150 160 170 180 m657 . pep TAPYQWCKAEDITEASAQFLPGILKTATLGYDGKGQIRVKTLDELKAAFAEHGGVDCVL M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M g657 TAPYQAVCKAEDITEASAQFLPGILKTATLGYDGKGQIRVKTLDELKAAFAEHGGVDCVL 130 140 150 160 170 180 190 200 210 220 230 240 m657. pep EKMVDLRSEISVIVCRLNNDNVQTFDPAENIHENGILAYSIVPARLSADVQQQARQMAQR I II I II I : II II II I II I : : I II II I II I II II I II II I I II II II II II I II II I III g657 EKMVDLRGEISVIVCRLNDENVQTFDPAENIHENGILAYSIVPARLSADVQQQARQTAQR 190 200 210 220 230 240 250 260 270 280 290 300 m657. pep LADELDYVGVLAVEMFWGDTHELWNEIAPRPΗNSGHHTIDACAADQFQQQVRIMCNLP I II II II II II II II II I II I II I : II I III II II II II II II II II I II II II II II g657 LADELDYVGVLAVEMFWGDTHELLVNETAPRTHNSGHHTIDACAADQFQQQVRIMCNLP 250 260 270 280 290 300 310 320 330 340 350 360 m657. pep PADTKLLSSCCMANILGDVWQEDGGEPDWLPLQSHPNAHLHLYGKKTAHKGRKMGHFTVL II I I II II II II I II I II I II I II II II II II I : II II II II II II I : I I II II II II I g657 PADTKLLSPCCMANILGDVWQEDGGEPDWLPLQSRPNAHLHLYGKKTAQKGRKMGHFTVL 310 320 330 340 350 360 370 379 m657.pep TTDSDTAFQEAKKLHQSLX I II II I II I II II II II I I g657 TTDSDTAFQEAKKLHQSLX 370 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2133>: a657. seq 1 ATGAAAAACA TATCTCTTTC TCCGCCCGCC ATGCTCGGCA TTCTTGGCGG 51 CGGACAATTA GGCAGAATGT TTACTGTTGC TGCCAAAACC ATGGGCTACA 101 AAGTAACCGT ACTCGATCCC AACCCGAATG CGCCGGCAGC GGAATTTGCC 151 GACCGCCATT TGTGTGCGCC GTTTGACAAC CAAACCGCTT TGGAAGAATT 201 GGCAAAATGT GCGGCTGTTA CGACCGAGTT CGAAAACGTC AATGCCGATG 251 CGATGCGTTT TCTCGCCAAA CATACCAATG TTTCCCCCAG CGGCGACTGC 301 GTTGCCATCG CGCAAAACCG CATTCAGGAA AAGGCATGGA TACGCAAAGC 351 AGGCCTGCAA ACCGCGCCGT ATCAAGCAAT TTGCAAAGCC GAAGACATCA 401 CTGAAGAAAG CATACAATTT CTGCCCGGCA TCCTGAAAAC CGCTACATTG 451 GGCTATGACG GCAAAGGCCA AATCCGCGTC AAAACGGTGG ATGAACTCAA 501 AGCCGCGTTT GCCGAACACC GCGGCGTGGA TTGCGTTTTG GAAAAAATGG 551 TGGACTTGCG CGGCGAAATT TCCGTTATCG TATGCCGTCT GAACAATGAC 601 AACGTGCAAA CTTTCGATCC TGCCGAAAAC ATTCACGAAA ACGGTATCCT 651 CGCCTACTCC ATCGTCCCAG CCCGACTGAG TGCCGACATT CAGCAACAGG 701 CGCGACAAAT GGCGCAGCGT TTGGCCGATG AATTGAACTA CGTCGGCGTA 751 TTGGCGGTAG AAATGTTTGT TGTCGGCGAC ACGCATGAAT TGGTCGTCAA 801 CGAAATCGCG CCGCGTCCGC ACAATTCCGG CCACCATACC GTCGACGCCT 851 GCGCGGCAGA CCAATTCCAG CAACAGGTCC GCCTGATGTG CAACCTGCCA 901 CCTGCTGACA CCAAATTGCT GAGTTCTTGC TGTATGGCGA ATATTTTGGG 951 CGACGTTTGG CAGGAAGACG GCGGCGAACC GGATTGGTTT CCCCTGCAAA 1001 GCCGGCCGGA CGCGCACCTG CACCTTTACG GCAAAAAAAC CGCGCACAAA 1051 GGGCGGAAAA TGGGACACTT TACCATTTTA AGCACCGATT CGGACACCGC 1101 ATTTCAAGAA GCAAAAAAAC TGCATCAGTC CCTATAA This corresponds to the amino acid sequence <SEQ ID 2134; ORF 657. a>: a657.pep 1 MKΝISLSPPA MLGILGGGQL GRMFTVAAKT MGYKVTVLDP ΝPΝAPAAEFA 51 DRHLCAPFDΝ QTALEELAKC AAVTTEFENV NADAMRFLAK HTNVSPSGDC 101 VAIAQNRIQE KAWIRKAGLQ TAPYQAICKA EDITEESIQF LPGILKTATL 151 GYDGKGQIRV KTVDELKAAF AEHRGVDCVL EKMVDLRGEI SVIVCRLNND 201 NVQTFDPAEN IHENGILAYS IVPARLSADI QQQARQMAQR LADELNYVGV 251 LAVEMFVVGD THELVVNEIA PRPHNSGHHT VDACAADQFQ QQVRLMCNLP 301 PADTKLLSSC CMANILGDVW QEDGGEPDWF PLQSRPDAHL HLYGKKTAHK 351 GRKMGHFTIL STDSDTAFQE AKKLHQSL* m657/a657 94.2% identity in 378 aa overlap 10 20 30 40 50 60 m657 •pep MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPDPDAPAAEFADRHLCAPFND I I II II II II II II II I II I II II II II II II II II II I I : I : II II II II II II II I:: a657 MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPNPNAPAAEFADRHLCAPFDN 10 20 30 40 50 60 70 80 90 100 110 120 m657.pep QAALDELAKCAAVTTEFENVNADAMRFLAKHTNVSPSGDCVAIAQNRIQEKAWIRKAGLQ MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a657 QTALEELAKCAAVTTEFENVNADAMRFLAKHTNVSPSGDCVAIAQNRIQEKAWIRKAGLQ 70 80 90 100 110 120 130 140 150 160 170 180 m657. pep TAPYQWCKAEDITEASAQFLPGILKTATLGYDGKGQIRVKTLDELKAAFAEHGGVDCVL I II I MM I II I II I I I II I II II II II II II II II I II I : II II II II II llllll a657 TAPYQAICKAEDITEESIQFLPGILKTATLGYDGKGQIRVKTVDELKAAFAEHRGVDCVL 130 140 150 160 170 180 190 200 210 220 230 240 m657.pep EKMVDLRSEISVIVCRLNNDNVQTFDPAENIHENGILAYSIVPARLSADVQQQARQMAQR MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a657 EKMVDLRGEISVIVCRLNNDNVQTFDPAENIHENGILAYSIVPARLSADIQQQARQMAQR 190 200 210 220 230 240 250 260 270 280 290 300 m657. pep LADELDYVGVLAVEMFVVGDTHELWNEIAPRPHNSGHHTIDACAADQFQQQVRIMCNLP II I I I : II I II I II II II I II II II I II II II II I II II I : II I II II II II I I : II II I a657 LADELNYVGVLAVEMFWGDTHELWNEIAPRPHNSGHHTVDACAADQFQQQVRLMCNLP 250 260 270 280 290 300 310 320 330 340 350 360 m657. pep PADTKLLSSCCMANILGDVWQEDGGEPDWLPLQSHPNAHLHLYGKKTAHKGRKMGHFTVL I II II II II II II I II I I I I II II II II I : I I II : I : II II II II II II II I II I II I: I a657 PADTKLLSSCCMANILGDVWQEDGGEPDWFPLQSRPDAHLHLYGKKTAHKGRKMGHFTIL 310 320 330 340 350 360 370 379 m657. pep TTDSDTAFQEAKKLHQSLX : I I I II II I I II II II II I a657 STDSDTAFQEAKKLHQSLX 370 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2135>: g658. seq 1 ATGGTGGCCG GAATTGTGCG TGCGCGGGGC GGTTTCATTG ACGAGCAATT 51 CATGTGTGTC GCCGACAACA AACATTTCTA CCGCCAAtac GCCGACATAA 101 TCCAATTCGT CCGCCAagcG TTGCGCCGTC TGCCGCGCCT GTTGCTGCAC 151 GTCGGCACTC AGCCGCGcgg gGACGATGga atAAGCCAAG ATGCCGTTTT 201 CGTGGATGTT TTCGGCGGGG TCGAAGGTTT GCACGTTTTC ATCGTTCAGA 251 CGGCATACGA TCACGGAAAT CTCGCCGCGC AAGTCCACCA TTTTTTCCAA 301 AACGCAATCC ACGCCGCCGT GTTCGGCAAA CGCGGCTTTG AGTTCGTCCA 351 ACGTTTTGAC GCGGATTTGA CCTTTGCCGT CGTAGCCCAA CGTAGCCGTT 401 TTCAGGATGC CGGGCAAAAA TTGCGCGCTT GCTTCAGTAA TGTCTTCGGC 451 CTTGCAAACC GCCTGATACG GCGCGGTTTG CAAGCCTGCT TTGCGTATCC 501 ACGCTTTTTC CTGAATGCGG TTTTGTGCAA TGGACACGCA GTCGCCGCTG 551 GGGGAAACGT TGGTATGCTT TGCCAGAGAG CGCATCGCGT CGGCAttgac 601 gtTTTCAAAT TCGGTcgtaA CCGCCGCGCA TTTTGCCAAT TCGTCCAACG 651 CGGCCCGGTC GTCAAACGGC GCGCACAAAT GGCGGTCGGC AAATTCCGCC 701 GCCGGCGCAT TCGGGTCGGG ATCGAGAACG GTTACTTTGT AGCCCATGGT 751 TTTAGCGGCA ACGGCAAACA TTctgcctAA This corresponds to the amino acid sequence <SEQ ID 2136; ORF 658.ng>: g658.pep 1 MVAGIVRARG GFIDEQFMCV ADΝKHFYRQY ADIIQFVRQA LRRLPRLLLH 51 VGTQPRGDDG ISQDAVFVDV FGGVEGLHVF IVQTAYDHGΝ LAAQVHHFFQ 101 NAIHAAVFGK RGFEFVQRFD ADLTFAWAQ RSRFQDAGQK LRACFSNVFG 151 LANRLIRRGL QACFAYPRFF LNAVLCNGHA VAAGGNVGML CQRAHRVGID 201 VFKFGRNRRA FCQFVQRGPV VKRRAQMAVG KFRRRRIRVG IENGYFVAHG 251 FSGNGKHSA* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2137>: m658.seq 1 ATGGTGTCCG GAATTGTGCG GGCGCGGGGC GATTTCGTTG ACGACCAATT 51 CATGCGTGTC ACCGACAACA AACATTTCTA CCGCCAATAC GCCGACATAA 101 TCCAATTCGT CCGCCAAGCG TTGCGCCATC TGCCGCGCCT GTTGCTGCAC 151 GTCGGCACTC AGTCGCGCGG GGACGATGGA ATAAGCCAAG ATGCCGTTTT 201 CGTGGATGTT TTCGGCAGGG TCGAAAGTTT GCACGTTGTC ATTGTTCAAA 251 CGGCATACGA TTACGGAAAT TTCACTGCGC AAATCCACCA TTTTTTCCAA 301 AACGCAATCC ACGCCGCCGT GTTCGGCAAA CGCGGCTTTG AGTTCATCCA 351 ATGTTTTTAC GCGGATTTGA CCTTTGCCGT CGTAGCCCAA CGTAGCCGTT 401 TTCAGGATGC CGGGCAAAAA TTGCGCGCTT GCTTCAGTGA TGTCTTCAGC 451 CTTACAAACC ACTTGATACG GCGCGGTTTG CAATCCCGCT TTGCGTATCC 501 ATGCCTTTTC CTGAATGCGG TTTTGTGCAA TCGCCACACA ATCGCCGCTA 551 GGGGAAACAT TGGTATGTTT TGCCAAAAAG CGCATCGCAT CGGCATTGAC 601 GTTTTCAAAT TCAGTGGTCA CCGCCGCGCA TTTTGCCAAT TCGTCCAAAG 651 CAGCTTGGTC GTTAAACGGC GCGCACAAAT GGCGGTCGGC AAATTCTGCT 701 GCCGGCGCGT CCGGATCGGG GTCGAGAACG GTTACTTTGT AGCCCATGGT 751 TTTGGCGGCA ACGGTAAACA TTCTGCCTAA This corresponds to the amino acid sequence <SEQ ID 2138; ORF 658>: m658.pep 1 MVSGIVRARG DFVDDQFMRV TDΝKHFYRQY ADIIQFVRQA LRHLPRLLLH 51 VGTQSRGDDG ISQDAVFVDV FGRVESLHW IVQTAYDYGΝ FTAQIHHFFQ 101 NAIHAAVFGK RGFEFIQCFY ADLTFAWAQ RSRFQDAGQK LRACFSDVFS 151 LTNHLIRRGL QSRFAYPCLF LNAVLCNRHT IAARGNIGMF CQKAHRIGID 201 VFKFSGHRRA FCQFVQSSLV VKRRAQMAVG KFCCRRVRIG VENGYFVAHG 251 FGGNGKHSA* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m658/g658 82.2% identity in 259 aa overlap 10 20 30 40 50 60 m658. pep MVSGIVRARGDFVDDQFMRVTDΝKHFYRQYADIIQFVRQALRHLPRLLLHVGTQSRGDDG II M II II II M M II I M I II II II II II II II II II II : II II II II II I IMM g658 MVAGIVRARGGFIDEQFMCVADΝKHFYRQYADIIQFVRQALRRLPRLLLHVGTQPRGDDG 10 20 30 40 50 60 70 80 90 100 110 120 m658. pep ISQDAVFVDVFGRVESLHWIVQTAYDYGΝFTAQIHHFFQΝAIHAAVFGKRGFEFIQCFY I II I II I I II II M M I II II II : I M : I I : II II I II II II II I II II II : I I g658 ISQDAVFVDVFGGVEGLHVFIVQTAYDHGΝLAAQVHHFFQΝAIHAAVFGKRGFEFVQRFD 70 80 90 100 110 120 130 140 150 160 170 180 m658. •pep ADLTFAWAQRSRFQDAGQKLRACFSDVFSLTΝHLIRRGLQSRFAYPCLFLΝAVLCΝRHT I II I I I II II II I II II II II II I II : I M I : M I II II II : II II : I I II II II M g658 ADLTFAWAQRSRFQDAGQKLRACFSΝVFGLAΝRLIRRGLQACFAYPRFFLΝAVLCΝGHA 130 140 150 160 170 180 190 200 210 220 230 240 m658 •pep IAARGΝIGMFCQKAHRIGIDVFKFSGHRRAFCQFVQSSLWKRRAQMAVGKFCCRRVRIG Ml IM I M II : II M II II I II : : I II II II II : II I I II I I I II II MMM g658 VAAGGΝVGMLCQRAHRVGIDVFKFGRΝRRAFCQFVQRGPWKRRAQMAVGKFRRRRIRVG 190 200 210 220 230 240 250 260 m658.pep VEΝGYFVAHGFGGΝGKHSAX : I I I I I I I I I I : I I I I I I I I g658 IENGYFVAHGFSGNGKHSAX 250 260 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2139>: a658. seq 1 ATGGTGGCCG GAATTGTGCG GACGCGGCGC GATTTCGTTG ACGACCAATT 51 CATGCGTGTC GCCGACAACA AACATTTCTA CCGCCAATAC GCCGACGTAG 101 TTCAATTCAT CGGCCAAACG CTGCGCCATT TGTCGCGCCT GTTGCTGAAT 151 GTCGGCACTC AGTCGGGCTG GGACGATGGA GTAGGCGAGG ATACCGTTTT 201 CGTGAATGTT TTCGGCAGGA TCGAAAGTTT GCACGTTGTC ATTGTTCAGA 251 CGGCATACGA TAACGGAAAT TTCGCCGCGC AAGTCCACCA TTTTTTCCAA 301 AACGCAATCC ACGCCGCGGT GTTCGGCAAA CGCGGCTTTG AGTTCATCCA 351 CCGTTTTGAC GCGGATTTGG CCTTTGCCGT CATAGCCCAA TGTAGCGGTT 401 TTCAGGATGC CGGGCAGAAA TTGTATGCTT TCTTCAGTGA TGTCTTCGGC 451 TTTGCAAATT GCTTGATACG GCGCGGTTTG CAGGCCTGCT TTGCGTATCC 501 ATGCCTTTTC CTGAATGCGG TTTTGCGCGA TGGCAACGCA GTCGCCGCTG 551 GGGGAAACAT TGGTATGTTT GGCGAGAAAA CGCATCGCAT CGGCATTGAC 601 GTTTTCGAAC TCGGTCGTAA CAGCCGCACA TTTTGCCAAT TCTTCCAAAG 651 CGGTTTGGTT GTCAAACGGC GCACACAAAT GGCGGTCGGC AAATTCCGCT 701 GCCGGCGCAT TCGGGTTGGG A CGAGTACG GTTACTTTGT AGCCCATGGT 751 TTTGGCAGCA ACAGTAAACA TTCTGCCTAA This corresponds to the amino acid sequence <SEQ ID 2140; ORF 658.a>: a658.pep 1 MVAGIVRTRR DFVDDQFMRV ADΝKHFYRQY ADWQFIGQT LRHLSRLLLΝ 51 VGTQSGWDDG VGEDTVFVΝV FGRIESLHW IVQTAYDΝGΝ FAAQVHHFFQ 101 NAIHAAVFGK RGFEFIHRFD ADLAFAVIAQ CSGFQDAGQK LYAFFSDVFG 151 FANCLIRRGL QACFAYPCLF LNAVLRDGNA VAAGGNIGMF GEKTHRIGID 201 VFELGRNSRT FCQFFQSGLV VKRRTQMAVG KFRCRRIRVG IEYGYFVAHG 251 FGSNSKHSA* m658/a658 75.3% identity in 259 aa overlap 10 20 30 40 50 60 m658. pep MVSGIVRARGDFVDDQFMRVTDNKHFYRQYADIIQFVRQALRHLPRLLLHVGTQSRGDDG I I : I II I : I II II II I II I : II II II II II I : : I M MMM I Ii I : II I II III a658 MVAGIVRTRRDFVDDQFMRVADNKHFYRQYADWQFIGQTLRHLSRLLLNVGTQSGWDDG 10 20 30 40 50 60 70 80 90 100 110 120 m658.pep ISQDAVFVDVFGRVESLHWIVQTAYDYGNFTAQIHHFFQNAIHAAVFGKRGFEFIQCFY :: : I : I I I : I I I M I I I I I I I I I I I I I I I I : I I : I I I I I I I I I I I I I I I I I I I I I : I a658 VGEDTVFVNVFGRIESLHWIVQTAYDNGNFAAQVHHFFQNAIHAAVFGKRGFEFIHRFD 70 80 90 100 110 120 130 140 150 160 170 180 m658. pep ADLTFAWAQRSRFQDAGQKLRACFSDVFSLTNHLIRRGLQSRFAYPCLFLNAVLCNRHT I I I : I I I : I I I I I I I I I I I I I I I I I :: : I l l l l l l l: I I I I I I I I I I I I : : : a658 ADLAFAVIAQCSGFQDAGQKLYAFFSDVFGFANCLIRRGLQACFAYPCLFLNAVLRDGNA 130 140 150 160 170 180 190 200 210 220 230 240 m658. pep IAARGNIGMFCQKAHRIGIDVFKFSGHRRAFCQFVQSSLWKRRAQMAVGKFCCRRVRIG M l I I I II I : I : I I I I I II I : : : : MM M I I : I I I II I : I I I I I I I I I I : I : I a658 VAAGGNIGMFGEKTHRIGIDVFELGRNSRTFCQFFQSGLWKRRTQMAVGKFRCRRIRVG 190 200 210 220 230 240 250 260 m658.pep VENGYFVAHGFGGNGKHSAX : I II I I II I II : I : II II I a658 IEYGYFVAHGFGSNSKHSAX 250 260 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2141 > • g661. seq 1 ATGCACATCG GCGGTTATTT TATCGACAAC CCCATCGCAC TTGCGCCGAT 51 GGCGGGCATT GCCGACAAAC CCTTCCGCCG CCTCTGTCGG GCGTTTGGCG 101 CAGGTTGGGC GGTGTGCGAA ATGCTGGCCA GCGATCCGAC GCTCAGGAAT 151 ACCGGAAAAA CCCtgcaccg cagtgaTTTt gccgatgaag gCGGCATCGT 201 TGCCGTGCAG ATTGCCGGCA GCGACCccga acaGATGGCG Gatgcggcgc 251 gttacAACGT CGGACTCGGG GCGCAGGTCA TCGACATcaa TATGGGCTGC 301 cccgccaaGA AAGTGTGCAA CGTCCAAGCC GGTAGCGCgc tGATGCAGGA 351 CGAGccgctg gttgcCgcca tTTtggaggc ggtggtcAAG GCGGCGGgcg 401 TACCCGTTAC cctCAAAACc cgtTtgggtt ggcacgacga cgatcaaaac 451 ctgcCcgccg tcgccaaaat cgccgaagat tgcggcattg ccgccCttgc 501 cgttccacgg gcgCGCgcgC ACGCAAATGT ACAAAGGCGA GGCgcGTTAC 551 Gaactcatcg CCGAGACCAA AAGccgTCTG AACATCCCGG cctGggtCAA 601 CGGCGACATC actTCgccgc AAAAAGCCGC CGccgTCCTC AAACAAACCG 651 CCGCCGACGG CATCATGATA GGGCGCGGCG CGCAAGGCAG GCCGTGGTTT 701 TTCCGCGATT TGAAGCATTA TGCCGAACAC GGCGTTTTAC CGCCTGCCTT 751 GAGTTTGGCA GAATGCAGAG CCGCCATTTT GAACCACATC CGCGCCATGC 801 ACGCGTTTTA TGGTGAGACC GTCGGTGTGC GCATCGCACG CAAACACATA 851 GGCTGGTACA TCGGCGAAAT GCCCGACGGC GAACAGGCGC GGCGTGA This corresponds to the amino acid sequence <SEQ ID 2142; ORF 66 l.ng>: gββl.pep 1 MHIGGYFIDN PIALAPMAGI ADKPFRRLCR AFGAGWAVCE MLASDPTLRN 51 TGKTLHRSDF ADEGGIVAVQ IAGSDPEQMA DAARYNVGLG AQVIDINMGC 101 PAKKVCNVQA GSALMQDEPL VAAILEAWK AAGVPVTLKT RLGWHDDDQN 151 LPAVAKIAED CGIAALAVPR ARAHANVQRR GALRTHRRDQ KPSEHPGLGQ 201 RRHHFAAKSR RRPQTNRRRR HHDRARRARQ AWFPRFEAL CRTRRFTACL 251 EFGRMQSRHF EPHPRHARVL WXDRRCAHRT QTHRLVHRRN ARRRTGAA* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2143>: 1 ATGCACATCG GCGGCTATTT TATCGACAAC CCCATCGCAC TTGCGCCGAT 51 GGCGGGCATT ACCGACAAAC CGTTCCGCCG ACTTTGCCGA GATTTTGGCG 101 CAGGTTGGGC GGTGTGCGAA ATGCTGACCA GCGACCCGAC GCTCAGAAAT 151 ACTAGAAAAA CCTTGCACCG CAGCGATTTT GCCGATGAAG GCGGCATTGT 201 TGCCGTGCAG ATTGCCGGAA GCGATCCGCA GCAGATGGCG GATGCCGCGC 251 GTTACAACGT CAGCCTTGGG GCGCAGCTTA TCGACATCAA CATGGGCTGT 301 CCCGCTAAAA AAGTCTGCAA TGTCCAAGCC GGTAGCGCGC TGATGCAGAA 351 CGAGCCGCTG GTTGCCGCCA TTTTGGAAGC CGTCGTCCGT GCGGCAGGCG 401 TACCCGTTAC CCTCAAAACC CGTTTGGGTT GGCACGACGA CCATCAAAAC 451 CTGCCCGTCA TCGCCAAAAT CGCCGAAGAT TGCGGCATCG CCGCCCTTGC 501 CGTCC.ACGG ACGCACGCGT ACGCAAATGT ACAAAGGCGA AGCGCGTTAC 551 GAACTCATCG CCGAAACCAA ATGCCGTCTG AACATCCCGG TCTGGGTCAA 601 CGGCGACATT ACTTCGCCGC AAAAAGCCCA AGCCGTCCTC AAACAAACCG 651 CCGCCGACGG CATTATGATA GGGCGCGGCG CGCAAGGCAG GCCGTGGTTC 701 TTCCGCGATT TGAAACATTA TGCCGAACAC GGTGTTTTGC CGCCTGCCTT 751 GAGTTTGGCA GAATGCGCCG CCGCTATTTT GAACCACATC CGCGCCATAC 801 ACGCGTTTTA CGGCGACACC GCCGGTGTGC GCATCGCACG CAAACACATA 851 GGCTGGTACA TCGACGAAAT GCCCGACGGC GAACAGACAC GTCGTGA This corresponds to the amino acid sequence <SEQ ID 2144; ORF 661>: m661.pep 1 MHIGGYFIDN PIALAPMAGI TDKPFRRLCR DFGAGWAVCE MLTSDPTLRN 51 TRKTLHRSDF ADEGGIVAVQ IAGSDPQQMA DAARYNVSLG AQLIDINMGC 101 PAKKVCNVQA GSALMQNEPL VAAILEA R AAGVPVTLKT RLGWHDDHQN 151 LPVIAKIAED CGIAALAVXR THAYANVQRR SALRTHRRNQ MPΞEHPGLGQ 201 RRHYFAAKSP SRPQTNRRRR HYDRARRARQ AWLPRFETL CRTRCFAACL 251 EFGRMRRRYF EPHPRHTRVL RRHRRCAHRT QTHRLVHRRN ARRRTDTS* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m661/g661 88.5% identity in 295 aa overlap 10 20 30 40 50 60 m661. pep MHIGGYFIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLTSDPTLRNTRKTLHRSDF I I I I I I I II I I II I I II I I I : I II I I I I I I II I I I I I I II I: I I I II I I I l l l ll l l l g661 MHIGGYFIDNPIALAPMAGIADKPFRRLCRAFGAGWAVCEMLASDPTLRNTGKTLHRSDF 10 20 30 40 50 60 70 80 90 100 110 120 m661. pep ADEGGIVAVQIAGSDPQQMADAARYNVSLGAQLIDINMGCPAKKVCNVQAGSALMQNEPL I I I I I I I I I I I I I I I I : I I I I I I I I I I : I I I I : I I I I I I I I I I I I I I I I I I I I I I I : I I I g661 ADEGGIVAVQIAGSDPEQMADAARYNVGLGAQVIDINMGCPAKKVCNVQAGSALMQDEPL 70 80 90 100 110 120 130 140 150 160 170 180 m661. pep VAAILEAWRAAGVPVTLKTRLGWHDDHQNLPVIAKIAEDCGIAALAVXRTHAYANVQRR I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I : : I I I I I I I I I I I I I I I : : I : I I I I I I g661 VAAILEAWKAAGVPVTLKTRLGWHDDDQNLPAVAKIAEDCGIAALAVPRARAHANVQRR 130 140 150 160 170 180 190 200 210 220 230 240 mδβl.pep SALRTHRRNQMPSEHPGLGQRRHYFAAKSPSRPQTNRRRRHYDRARRARQAWLPRFETL : I I I I I I I : I M M M M M M M M M I I I I I I I I I M I I I I I I I I I I M I I I I : I g661 GALRTHRRDQKPSEHPGLGQRRHHFAAKSRRRPQTNRRRRHHDRARRARQAWFPRFEAL 190 200 210 220 230 240 250 260 270 280 290 299 m661. pep CRTRCFAACLEFGRMRRRYFEPHPRHTRVLRRHRRCAHRTQTHRLVHRRNARRRTDTSX l lll I : II II II II : M I I I I I I I : I I I I I I I I I I I II II I I I I I I I I I I g661 CRTRRFTACLEFGRMQSRHFEPHPRHARVLWXDRRCAHRTQTHRLVHRRNARRRTGAAX 250 260 270 280 290 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2145>: aδβl.seq 1 ATGCACATCG GCGGCTATTT TATCGACAAC CCCATCGCAC TTGCGCCGAT 51 GGCGGGCATT ACCGACAAAC CGTTCCGCCG ACTTTGCCGA GATTTTGGCG 101 CAGGTTGGGC GGTGTGCGAA ATGCTGACCA GCGACCCGAC GCTCAGAAAT 151 ACTAGAAAAA CCTTGCACCG CAGCGATTTT GCCGATGAAG GCGGCATTGT 201 TGCCGTGCAG ATTGCCGGAA GCGATCCGCA GCAGATGGCG GATGCCGCGC 251 GTTACAACGT CAGCCTTGGG GCGCAGCTTA TCGACATCAA CATGGGCTGT 301 CCCGCTAAAA AAGTCTGCAA TGTCCAAGCC GGTAGCGCGC TGATGCAGAA 351 CGAGCCGCTG GTTGCCGCCA TTTTGGAGGC GGTGGTCAAA GCGGCGGGCG 401 TACCCGTTAC CCTCAAAACC CGTTTGGGTT GGCACGACGA CCATCAAAAC 451 CTGCCCGTCA TCGCCAAAAT CGCCGAAGAT TGCGGCATTG CCGCCCTTGC 501 CG.TCCACGG ACGCACGCGC ACGCAAATGT ACAAAGGCGA AGCGGCTTAC 551 GACCTGATTG CCGAAACCAA ATGCCGTCTG AACATCCCGG TCTGGGTCAA 601 CGGCGACATT ACCTCGCCGC AAAAAGCCCA AGCCGTCCTC AAACAAACCG 651 CCGCAGACGG CATTATGATA GGGCGCGGCG CGCAAGGCAG ACCGTGGTTC 701 TTCCGCGATT TGAAACATTA CGCCGAACAC GGTGTTTTAC CGCCTGCCTT 751 GAGTTTGGCA GAATGTACCG CCACTATTTT GAACCACATC CGAGCCATGC 801 ACGCGTTTTA CGGCGACACC GCCGGTGTGC GCATCGCACG CAAACACATA 851 GGCTGGTACA TCGACGAAAT GCCCGACGGC GAACAGACAC GTCGTGA This corresponds to the amino acid sequence <SEQ ID 2146; ORF 661. a>: 1 MHIGGYFIDN PIALAPMAGI TDKPFRRLCR DFGAGWAVCE MLTSDPTLRN 51 TRKTLHRSDF ADEGGIVAVQ IAGSDPQQMA DAARYNVSLG AQLIDINMGC 101 PAKKVCNVQA GSALMQNEPL VAAILEAWK AAGVPVTLKT RLGWHDDHQN 151 LPVIAKIAED CGIAALAXPR THAHANVQRR SGLRPDCRNQ MPSEHPGLGQ 201 RRHYLAAKSP SRPQTNRRRR HYDRARRARQ TWLPRFETL RRTRCFTACL 251 EFGRMYRHYF EPHPSHARVL RRHRRCAHRT QTHRLVHRRN ARRRTDTS* m661/a661 94.6% identity in 298 aa overlap 10 20 30 40 50 60 m661. pep MHIGGYFIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLTSDPTLRNTRKTLHRSDF II II II I I II II II II II II II II II I I II I I II II I II II II II II II II I II II II I I a661 MHIGGYFIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLTSDPTLRNTRKTLHRSDF 10 20 30 40 50 60 70 80 90 100 110 120 m661. pep ADEGGIVAVQIAGSDPQQMADAARYNVSLGAQLIDINMGCPAKKVCNVQAGSALMQNEPL M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M a661 ADEGGIVAVQIAGSDPQQMADAARYNVSLGAQLIDINMGCPAKKVCNVQAGSALMQNEPL 70 80 90 100 110 120 130 140 150 160 170 180 m661. pep VAAILEAWRAAGVPVTLKTRLGWHDDHQNLPVIAKIAEDCGIAALAVXRTHAYANVQRR I II I II I II : II I II II II II I II I II I II II II II I II II II II II I II M II I II I a661 VAAILEAWKAAGVPVTLKTRLGWHDDHQNLPVIAKIAEDCGIAALAXPRTHAHANVQRR 130 140 150 160 170 180 190 200 210 220 230 240 m661. pep SALRTHRRNQMPSEHPGLGQRRHYFAAKSPSRPQTNRRRRHYDRARRARQAWLPRFETL M II II I II II II II II I I : II II II I I II I I II II II II II II I : II II II I II a661 SGLRPDCRNQMPSEHPGLGQRRHYLAAKSPSRPQTNRRRRHYDRARRARQTWLPRFETL 190 200 210 220 230 240 250 260 270 280 290 299 m661. pep CRTRCFAACLEFGRMRRRYFEPHPRHTRVLRRHRRCAHRTQTHRLVHRRNARRRTDTSX I I I I I : I I I I I I I I I : II I I I I M II II II I I I I I II II I I I I I I I II I II II I I I a661 RRTRCFTACLEFGRMYRHYFEPHPSHARVLRRHRRCAHRTQTHRLVHRRNARRRTDTSX 250 260 270 280 290 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2147>: g663. seq 1 ATGTGTACCG AGATGAAATT TATATTTTTT GTACTGTATG TTTTGCAGTT 51 TCTGCCGTTT GCGCTGCTGC ACAAGATTGC CGGCCTGATC GGTTCGCTTG 101 CCTACCTTCT GGTCAAACCG CGCCGCCGTA TCGGCGAAAT CAATTTGGCA 151 AAATGTTTTC CCGAATGGGA CGAAGAAAAG CGTAAAACCG TGTTGAAACA 201 GCATTTCAAA CACATGGCAA AACTGATGCT CGAATACGGC TTATATTGGT 251 ACGCGtctGC CAAATGCCTG AAATCGCTGG TGCGCTACCG CAATAAGCAT 301 TATTTGGACG ACGCGCTGGC GGCGGGGGAA AAAGTCATCA TCCTGTACCC 351 GCACTTTACC GCGTTCGAGA TGGCGGTGTA CGCGCTTAAT CAGGATGTCC 401 CGCTGATCAG TATGTATTCC CACCAAAAAA ACAAGATATT GGACGAACAG 451 ATTTTGAAAg gccgcaACCG CTATCACAAC GTCTTCCTTA TCGGGCGCAC 501 CGAagggctg cgCGCCCtcg TCAAACAGTT CCGCAAAAGC AGTGCGCCGT 551 TCCTGTATCT GCCCGATCAG GATTTCGGAC GCAACAATTC GGTTTTTGTG 601 GATTTTTTCG GCATtcagaC GGCAACGATT ACCGGCTTGA GCCGCATTGC 651 CGCGCTTGCA AATGCAAAAG TGATACCCGC CATTCCCGTC CGCGAGGCGG 701 ACAATACGGT TACATTGCAA TTCTATCCCG CTTGGAAATC CTTTCCGAGT 751 GAAGACGCGC AAGCCGACGC GCAACGTATG AACCGCTTTA TCGAAGAACG 801 CGTGCGCGAA CACCCGGAAC AATATTTCTG GCTGCACAAG CGTTTCAAAA 851 CCCGTCCGGA AGGCAGCCCC GATTTTTACT GA This corresponds to the amino acid sequence <SEQ ID 2148; ORF 663.ng>: g663.pep 1 MCTEMKFIFF VLYVLQFLPF ALLHKIAGLI GSLAYLLVKP RRRIGEIΝLA 51 KCFPEWDEEK RKTVLKQHFK HMAKLMLEYG LYWYASAKCL KSLVRYRΝKH 101 YLDDALAAGE KVIILYPHFT AFEMAVYALΝ QDVPLISMYS HQKΝKILDEQ 151 ILKGRΝRYHΝ VFLIGRTEGL RALVKQFRKS SAPFLYLPDQ DFGRΝΝSVFV 201 DFFGIQTATI TGLSRIAALA ΝAKVIPAIPV READΝTVTLQ FYPAWKSFPS 251 EDAQADAQRM ΝRFIEERVRE HPEQYFWLHK RFKTRPEGSP DFY* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2149>: m663. seq 1 ATGTGTATCG AGATGAAATT TATATTTTTT GTACTGTATG TTTTGCAGTT 51 TCTGCCGTTT GCGCTGCTGC ACAAGATTGC CGACCTGACG GGTTTGCTTG 101 CCTACCTTCT GGTCAAACCG CGCCGCCGTA TCGGCGAAAT CAATTTGGCA 151 AAATGTTTTT CCGAATGGAG TGAGGAAAAG CGTAAAACCG TGTTGAAACA 201 GCATTTCAAA CACATGGCGA AACTGATGTT GGAATACGGT TTATATTGGT 251 ACGCGCCTGC CGGACGTTTG AAATCGCTGG TGCGCTACCG CAATAAGCAT 301 TATTTGGACG ACGCGCTGGC GGCGGGGGAA AAAGTCATCA TCCTGTATCC 351 GCACTTCACC GCGTTCGAGA TGGCGGTGTA CGCGCTTAAT CAGGATATCC 401 CGCTGATCAG TATGTATTCC CATCAAAAAA ACAAGATATT GGACGAACAG 451 ATTTTGAAAG GCCGCAACCG CTATCACAAC GTCTTCCTTA TCGGGCGCAC 501 CGAAGGGCTG CGCGCCCTCG TCAAACAGTT CCGCAAAAGC AGCGCGCCGT 551 TTCTGTATCT GCCCGATCAG GATTTCGGAC GCAACGATTC GGTTTTTGTG 601 GATTTTTTCG GTATTCAGAC GGCAACGATT ACCGGATTGA GCCGCATTGC 651 CGCGCTTGCA AATGCAAAAG TGATACCCGC CATTCCCGTC CGCGAGGCAG 701 ACAATACGGT TACATTGCAT TTCTACCCTG CTTGGAAATC CTTTCCGGGT 751 GAAGACGCGA AAGCCGACGC GCAGCGCATG AACCGTTTTA TCGAAGACAG 801 GGTGCGCGAA CATCCGGAAC AATATTTTTG GCTGCACAAG CGTTTTAAAA 851 CCCGTCCGGA AGGCAGCCCC GATTTTTACT GA This corresponds to the amino acid sequence <SEQ ID 2150; ORF 663>: m663.pep 1 MCIEMKFIFF VLYVLQFLPF ALLHKIADLT GLLAYLLVKP RRRIGEINLA 51 KCFSEWSEEK RKTVLKQHFK HMAKLMLEYG LYWYAPAGRL KSLVRYRNKH 101 YLDDALAAGE KVIILYPHFT AFEMAVYALN QDIPLISMYS HQKNKILDEQ 151 ILKGRNRYHN VFLIGRTEGL RALVKQFRKS SAPFLYLPDQ DFGRNDSVFV 201 DFFGIQTATI TGLSRIAALA NAKVIPAIPV READNTVTLH FYPAWKSFPG 251 EDAKADAQRM NRFIEDRVRE HPEQYFWLHK RFKTRPEGSP DFY* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m663/g663 94.9% identity in 293 aa overlap 10 20 30 40 50 60 m663.pep MCIEMKFIFFVLYVLQFLPFALLHKIADLTGLLAYLLVKPRRRIGEIΝLAKCFSEWSEEK I I M M M M M M M M M M M M I I I I I I I I I I I I I I I I I I I I I I I M M g663 MCTEMKFIFFVLYVLQFLPFALLHKIAGLIGSLAYLLVKPRRRIGEIΝLAKCFPEWDEEK 10 20 30 40 50 60 70 80 90 100 110 120 m663. pep RKTVLKQHFKHMAKLMLEYGLYWYAPAGRLKSLVRYRΝKHYLDDALAAGEKVIILYPHFT II II II I II II II II II I I II II I I I II II I II I II I II II I II II II II II II II I g663 RKTVLKQHFKHMAKLMLE GLYWYASAKCLKSLVRYRΝKHYLDDALAAGEKVIILYPHFT 70 80 90 100 110 120 130 140 150 160 170 180 m663.pep AFEMAVYALΝQDIPLISMYSHQKΝKILDEQILKGRΝRYHΝVFLIGRTEGLRALVKQFRKS II II II II II II : I II II I II II II I II I I II II I I II I II II II II II II II II II I M g663 AFEMAVYALΝQDVPLISMYSHQKΝKILDEQILKGRΝRYHΝVFLIGRTEGLRALVKQFRKS 130 140 150 160 170 180 190 200 210 220 230 240 rtl663.pep SAPFLYLPDQDFGRΝDSVFVDFFGIQTATITGLSRIAALAΝAKVIPAIPVREADΝTVTLH MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g663 SAPFLYLPDQDFGRΝΝSVFVDFFGIQTATITGLSRIAALAΝAKVIPAIPVREADΝTVTLQ 190 200 210 220 230 240 250 260 270 280 290 m663.pep FYPAWKSFPGEDAKADAQRMΝRFIEDRVREHPEQYFWLHKRFKTRPEGSPDFYX II I II I II M II I : II II I II II II : I II I I I II II II II I II II I II II II II g663 FYPAWKSFPSEDAQADAQRMΝRFIEERVREHPEQYFWLHKRFKTRPEGSPDFYX 250 260 270 280 290 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2151>: a663.seq 1 ATGTGTATCG AGATGAAATT TATATTTTTT GTACTGTATG TTTTGCAGTT 51 TCTGCCGTTT GCGCTGCTGC ACAAACTTGC TGATCTGACA GGCTTGCTCG 101 CCTACCTTTT GGTCAAACCC CGCCGCCGTA TCGGCGAAAT CAATTTGGCA 151 AAATGCTTTC CCGAGTGGGA CGGAAAAAAG CGTAAAACCG TGTTGAAACA 201 GCATTTCAAA CATATGGCGA AACTGATGTT GGAATACGGT TTATATTGGT 251 ACGCGCCCGC CGGGCGTTTG AAATCACTGG TGCGCTACCG CAACAAACAT 301 TATTTGGACG ACGCTCTGGC GGCAGGGGAA AAAGTCATCA TCCTGTATCC 351 GCACTTCACC GCGTTCGAGA TGGCGGTGTA CGCGCTCAAT CAGGATGTTC 401 CGCTGATCAG TATGTATTCC CACCAAAAAA ACAAGATATT GGACGAACAG 451 ATTTTGAAAG GCCGCAACCG CTATCACAAC GTTTTCCTTA TCGGGCGCAC 501 CGAAGGGCTG CGCGCCCTCG TCAAACAGTT CCGCAAAAGC AGCGCGCCGT 551 TTCTGTATCT GCCCGATCAG GATTTCGGAC GCAACGATTC GGTTTTTGTC 601 GATTTCTTCG GTATTCGGAC GGCAACGATT ACCGGCTTGA GCCGCATTGC 651 CGCGCTTGCA AATGCAAAAG TGATACCCGC CATCCCTGTC CGCGAGGCGG 701 ACAATACGGT TACATTGCAT TTCTACCCTG CTTGGGAATC CTTTCCGAGT 751 GAAGATGCGC AGGCCGACGC GCAGCGCATG AACCGTTTTA TCGAGGAACG 801 CGTGCGCGAA CATCCCGAGC AGTATTTTTG GCTGCACAAG CGTTTCAAAA 851 CCCGTCCGGA AGGCAGCCCC GATTTTTACT GA This corresponds to the amino acid sequence <SEQ ID 2152; ORF 663. a>: a663.pep 1 MCIEMKFIFF VLYVLQFLPF ALLHKLADLT GLLAYLLVKP RRRIGEINLA 51 KCFPEWDGKK RKTVLKQHFK HMAKLMLEYG LYWYAPAGRL KSLVRYRNKH 101 YLDDALAAGE KVIILYPHFT AFEMAVYALN QDVPLISMYS HQKNKILDEQ 151 ILKGRNRYHN VFLIGRTEGL RALVKQFRKS SAPFLYLPDQ DFGRNDSVFV 201 DFFGIRTATI TGLSRIAALA NAKVIPAIPV READNTVTLH FYPAWEΞFPS 251 EDAQADAQRM NRFIEERVRE HPEQYFWLHK RFKTRPEGSP DFY* 663/a663 96.2% identity in 293 aa overlap 10 20 30 40 50 60 m663.pep MCIEMKFIFFVLYVLQFLPFALLHKIADLTGLLAYLLVKPRRRIGEINLAKCFSEWSEEK I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I a663 MCIEMKFIFFVLYVLQFLPFALLHKLADLTGLLAYLLVKPRRRIGEINLAKCFPEWDGKK 10 20 30 40 50 60 70 80 90 100 110 120 m663.pep RKTVLKQHFKHMAKLMLEYGLYWYAPAGRLKSLVRYRNKHYLDDALAAGEKVIILYPHFT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a663 RKTVLKQHFKHMAKLMLEYGLYWYAPAGRLKSLVRYRNKHYLDDALAAGEKVIILYPHFT 70 80 90 100 110 120 130 140 150 160 170 180 m663.pep AFEMAVYALNQDIPLISMYSHQKNKILDEQILKGRNRYHNVFLIGRTEGLRALVKQFRKS I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I a663 AFEMAVYALNQDVPLISMYSHQKNKILDEQILKGRNRYHNVFLIGRTEGLRALVKQFRKS 130 140 150 160 170 180 190 200 210 220 230 240 m663.pep SAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANAKVIPAIPVREADNTVTLH I I I I I I I I I I I I I I I I I I I I I II I l I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I a663 SAPFLYLPDQDFGRNDSVFVDFFGIRTATITGLSRIAALANAKVIPAIPVREADNTVTLH 190 200 210 220 230 240 250 260 270 280 290 m663.pep FYPAWKSFPGEDAKADAQRMNRFIEDRVREHPEQYFWLHKRFKTRPEGSPDFYX I I I I M I I I M I I M I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I a663 FYPAWESFPΞEDAQADAQRMNRFIEERVREHPEQYFWLHKRFKTRPEGSPDFYX 250 260 270 280 290 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2153>: g664. seq 1 ATGATACATC CGCACCACTT CCGCGCCTTT TTCATAAACG GTCATGGTGT 51 AGAAATTGTT CATCTCCTCA TAGCTGAcgg gGCGCACCGG ATGGGCGGTC 101 GGGCCTGCGT CTTCGGGGAA CTGGTTCTGG CGCAGCAGGC GGATGTTCTC 151 GATGCGGCGC ACGGCGCGGC CGGCGCGGTC GCCGGAAAAC TCTTGGTCGC 201 GGAACACGGT CAGCCCTTCC TTCAGCGAAA GCTGGAACCA GTCGCGGCAG 251 GTTACGCGGT TGCCCGTCCA GTTGTGGAAA TATTCGTGTC CGACCACGGA 301 TTCAATGCCT TCGAAATCGG TATCGGTGGC GGTGCGGCTG TCGGCGAGGA 351 CGAACTTGGT GTTAAAAATG TTCAAACCCT TGTTTTCCAT CGCGCCCATA 401 TTGAAATCGC CTACGGCGAC GACCATGAaa atatccaagt cataTTCcaa 451 cCcgaagcgc gtttcgtcCc acttcatcgC gtTTTTTCAA cgaTTCCACG 501 GCAAAGCCGA CCTTGGGTTT GTCCGCTTCG GTGGTGTAAA ACTCGATTTT 551 GA This corresponds to the amino acid sequence <SEQ ID 2154; ORF 664.ng>: g664.pep 1 MIHPHHFRAF FINGHGVEIV HLLIADGAHR MGGRACVFGE LVLAQQADVL 51 DAAHGAAGAV AGKLLVAEHG QPFLQRKLEP VAAGYAVARP WEIFVSDHG 101 FNAFEIGIGG GAAVGEDELG VKNVQTLVFH RAHIEIAYGD DHENIQVIFQ 151 PEARFVPLHR VFSTIPRQSR PWVCPLRWCK TRF* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2155>: m664. seq 1 GTGATACATC CGCACTACTT CCGCGCCTTT TTCATAAACG GTCATGGTGT 51 AGAAATTGTT CATCTCCTCA TAGCTGGCGG GGCGCACCGG ATGGGCGGTC 101 GGGCCTGCGT CTTCGGGGAA CTGGTGCTGG CGCAGCAGGC GGATGTTTTC 151 GATGCGGCGC ACGGCGCGGC TGGCGCGGTC GCCGGAAAAT TCTTGGTCGC 201 GGAACACGGT CAGCCCTTCC TTCAGCGAAA GCTGGAACCA GTCGCGGCAG 251 GTTACGCGGT TGCCCGTCCA GTTGTGGAAA TACTCGTGTC CGACCACGGA 301 TTCGATGCCT TCGAAATCGG TATCGGTGGC GGTGCGGCTG TCGGCAAGGA 351 CGAACTTGGT GTTAAAGATG TTCAAACCCT TGTTTTCCAT CGCGCCCATA 401 TTGAAATCGC CCACGGCGAC GACCATGAAA ATATCCAAGT CGTATTCCAA 451 ACCGAAGCGC GTTTCGTCCC ATTTCATCGC GTTTTT . CAA CGATTCCACG 501 GCAAAGCCGA CCTTGGGCTT GTCCGCTTCG GTGGTGTAAA ACTCGATTTT 551 GA This corresponds to the amino acid sequence <SEQ ID 2156; ORF 664>: m664.pep 1 VIHPHYFRAF FINGHGVEIV HLLIAGGAHR MGGRACVFGE LVLAQQADVF 51 DAAHGAAGAV AGKFLVAEHG QPFLQRKLEP VAAGYAVARP WEILVSDHG 101 FDAFEIGIGG GAAVGKDELG VKDVQTLVFH RAHIEIAHGD DHENIQVVFQ 151 TEARFVPFHR VFXTIPRQSR PWACPLRWCK TRF* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m664/g664 91.8% identity in 183 aa overlap 10 20 30 40 50 60 m664.pep VIHPHYFRAFFIΝGHGVEIVHLLIAGGAHRMGGRACVFGELVLAQQADVFDAAHGAAGAV : I II M II II II II I II II II II II II II II II II II II I II II II I M II I I I I II II g664 MIHPHHFRAFFIΝGHGVEIVHLLIADGAHRMGGRACVFGELVLAQQADVLDAAHGAAGAV 10 20 30 40 50 60 70 80 90 100 110 120 m664.pep AGKFLVAEHGQPFLQRKLEPVAAGYAVARPWEILVSDHGFDAFEIGIGGGAAVGKDELG I I M II II I I II II I I I I II II II I I I II II I II : II I II I: II II II II II II I: II I I g664 AGKLLVAEHGQPFLQRKLEPVAAGYAVARPWEIFVSDHGFΝAFEIGIGGGAAVGEDELG 70 80 90 100 110 120 130 140 150 160 170 180 m664. pep VKDVQTLVFHRAHIEIAHGDDHEΝIQWFQTEARFVPFHRVFXTIPRQSRPWACPLRWCK II : II I II I II II I II I : I II I II I IM I I II II I M II I I II I II II I M II II I II g664 VKΝVQTLVFHRAHIEIAYGDDHEΝIQVIFQPEARFVPLHRVFSTIPRQSRPWVCPLRWCK 130 140 150 160 170 180 m664.pep TRFX II II g664 TRFX The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2157>: a664. seq 1 GTGATACATC CGCACCACTT CCGCGCCTTT TTCATAAACG GTCATGGTGT 51 AGAAATTGTT CATCTCCTCA TATCGGGCGG GGCGCACCGG ATGTGCGGTC 101 GGACCTGCGT CTTCGGGGAA CTGGTGCTGG CGCAGCAGGC GGATGTTTTC 151 GATACGGCGC ACGGCGCGGC TGGCGCGGTC GCCGGAAAAT TCTTGGTCGC 201 GGAACACGGT CAACCCTTCC TTCAGCGAAA GCTGGAACCA GTCGCGGCAG 251 GTCACGCGGT TGCCCGTCCA GTTGTGGAAA TATTCGTGTC CGACCACGGA 301 TTCGATGCCT TCAAAATCGG TATCGGTGGC GGTACGGCTG TCGGCAAGGA 351 CGAACTTGGT GTTAAAGATG TTCAAACCCT TGTTTTCCAT CGCACCCATA 401 TTGAAATCGC CCACGGCGAC GACCATGAAA ATATCCAAGT CGTATTCCAA 451 ACCGAAGCGC GTTTCGTCCC ACTTCATTGC GTTTTT . CAG CGATTCCACG 501 GCAAAGCCGA CCTTGGGCTT GTCCGCTTCG GTGGTGTAAA ACTCGATTTT 551 GA This corresponds to the amino acid sequence <SEQ ID 2158; ORF 664. a>: a664.pep 1 VIHPHHFRAF FINGHGVEIV HLLISGGAHR MCGRTCVFGE LVLAQQADVF 51 DTAHGAAGAV AGKFLVAEHG QPFLQRKLEP VAAGHAVARP WEIFVSDHG 101 FDAFKIGIGG GTAVGKDELG VKDVQTLVFH RTHIEIAHGD DHENIQVVFQ 151 TEARFVPLHC VFXAIPRQSR PWACPLRWCK TRF* m664/a664 92.9% identity in 183 aa overlap 10 20 30 40 50 60 m664.pep VIHPHYFRAFFINGHGVEIVHLLIAGGAHRMGGRACVFGELVLAQQADVFDAAHGAAGAV II II I : II II II II II II II II I I: II I II I II : I II II II I II I II II I : I II II I II a664 VIHPHHFRAFFINGHGVEIVHLLISGGAHRMCGRTCVFGELVLAQQADVFDTAHGAAGAV 10 20 30 40 50 60 70 80 90 100 110 120 m664. pep AGKFLVAEHGQPFLQRKLEPVAAGYAVARPWEILVSDHGFDAFEIGIGGGAAVGKDELG II I I II II II II II I II I I II I II : I I II I I II I : I II II II I I : I II II I : I I II I I I I a664 AGKFLVAEHGQPFLQRKLEPVAAGHAVARPWEIFVSDHGFDAFKIGIGGGTAVGKDELG 70 80 90 100 110 120 130 140 150 160 170 180 m664.pep VKDVQTLVFHRAHIEIAHGDDHENIQWFQTEARFVPFHRVFXTIPRQSRPWACPLRWCK I II II II I II I : I II II II II II I II II II I II I II I : I II I : II II II I II II II I II a664 VKDVQTLVFHRTHIEIAHGDDHENIQVVFQTEARFVPLHCVFXAIPRQSRPWACPLRWCK 130 140 150 160 170 180 m664.pep TRFX II II a664 TRFX The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2159>: g665. seq 1 atgaagtgGg acgaaacgcg cttcgGgttg GAAtatgact tggatatttT 51 CATGGTCGTC GCCGTAGGCG ATTTCAATAT GGGCGCGATG GAAAACAAGG 101 GTTTGAACAT TTTTAACACC AAGTTCGTCC TCGCCGACAG CCGCACCGCC 151 ACCGATACCG ATTTCGAAGG CATTGAATCC GTGGTCGGAC ACGAATATTT 201 CCACAACTGG ACGGGCAACC GCGTAACCTG CCGCGACTGG TTCCAGCTTT 251 CGCTGAAGGA AGGGCTGACC GTGTTCCGCG ACCAAGAGTT TTCCGGCGAC 301 CGCGCCGGCC GCGCCGTGCG CCGCATCGAG AACATCCGCC TGCTGCGCCA 351 GAACCAGTTC CCCGAAGACG CAGGCCCGAC CGCCCATCCG GTGCGCcccg 401 TCAGCTATGA GGAGATGAAC AATTTCTACA CCATGACCGT TTATGAAAAA 451 GGCGCGGAAG TGGTGCGGAT GTATCATACC CTGCTCGGCG AAGAGGGCTT 501 CCAAAAAGGC ATGAAGCTAT ATTTCcaacg CCACGACGGA CAGGCAGTGA 551 CCTGCGACGA TTTCCGCGCG GCGatggcgg ATGCGAACGG CATCAATCTC 601 GACCAGTTCG CCTTGTGGTA CAGCCAGGCG GGCACGCCCG TTTTGGAAGC 651 CGAAGGCCGT CTGAAAAACA ATGTTTTCGA GTTAACCATT AAACAAACCG 701 TGCCGCCCAC GCCCGATATG GCGGACAAAC AGCCGATGAT GATTCCCGTC 751 AAAGTCGGGC TTCTGAACCG CAACGGCGAA GCGGTGGCAT TCGATTATCA 801 GGGCAAACGC GCAACCGAAG CCGTGTTGCT GATGACCGAA GCCGAACagg 851 CCTTCCCGCT CGAAGGTGTA ACCGAAGCCG TCGTTCCCTC GCTGCTGCGC 901 GGGTTCAGCG CGCCAGTGTA TCTGAACTAT CCGTACAGCG ACGACGACCT 951 GCTGCTCCTG CTCGCCCACG ACAGCGACGC TTTCACGTGC TGGGAAGCCG 1001 CCCAAACGCT CTACCGTCGC GCCGTCGCCG CCAACCTTGC CGCGCTTTCA 1051 GACGGCATCG GGTTGCCGAA ACACGAAAAA CTGCTTGCCG CCGTCGAAAA 1101 AGTCATTTCA GACGACCTCT TGGACAACGC CTTCAAAGCC CTGCTTTTGG 1151 GCGTGCCGTC CGAAGCCGAa ctGTGGGACG GCACGGAAAA CATcgaCCCG 1201 CTGCGCTACC ATCAGGCGCG CGAAGCCTTG TTGGATACGC TTGCCGtcCG 1251 CttcctgcCG AAATGGCACG AATTGGaccg tcaggcggcg aagCAggaaa 1301 accaaagtTA CGAATACAGC CCCGAAACCG CCGACTGGCG CACGCTGCGC 1351 AACGTCTGCC GCGCCTtcgt cctGCGCGCC GACCCCGCGC acatcgAAAC 1401 TGTTGCCGAA Aaatacggcg AAATGGCGCA AAACATGACC CACGAATGGG 1451 GCATCCTGTC CGCCGTCAAC GGCAACGAAA GCGATACGCG CAACTGCCTG 1501 CTGGCGCAGT TTGCCGAcaa gTtttcAGAC GACGCGCTGG TGATGGACAA 1551 ATATTTCGCC CTTATCGGCT CAAGccgccg cagCGACACC CTGCAACAGG 1601 TTCAAACCGC CTTGCAGCAT CCGAAATTCA GTCTCGAAAA CCCCAACAAA 1651 GCCCGTTCGC TCATCGGCAG CTTCAGCCGC AACGTCCCGC ATTTTCACGC 1701 ACAAGACGGC AGCGGCTACC GCTTCATCGC CGACAAAGTC ATCGAAATCG 1751 ACCGCTTCAA cCCGCAggtc gccGCCCGCC TGGTGCAGGC GTTCAACCTC 1801 TGCAACAAGC TCGAGCCGCA CCGCAAAAAC TTgGTGAAAC AAGAATTGCA 1851 GTGCATTCGG GCGCAGGAAG GATTGTCGAA AGacGTGGGC GAaatcgtCG 1901 GCAAGATTTT GGGTTGA This corresponds to the amino acid sequence <SEQ ID 2160; ORF 665. ng>: g665.pep 1 MKWDETRFGL EYDLDIFMW AVGDFNMGAM ENKGLNIFNT KFVLADSRTA 51 TDTDFEGIES VVGHEYFHNW TGNRVTCRDW FQLSLKEGLT VFRDQEFSGD 101 RAGRAVRRIE NIRLLRQNQF PEDAGPTAHP VRPVSYEEMN NFYTMTVYEK 151 GAEWRMYHT LLGEEGFQKG MKLYFQRHDG QAVTCDDFRA AMADANGINL 201 DQFALWYSQA GTPVLEAEGR LKNNVFELTI KQTVPPTPDM ADKQPMMIPV 251 KVGLLNRNGE AVAFDYQGKR ATEAVLLMTE AEQAFPLEGV TEAWPSLLR 301 GFSAPVYLNY PYΞDDDLLLL LAHDSDAFTC WEAAQTLYRR AVAANLAALS 351 DGIGLPKHEK LLAAVEKVIS DDLLDNAFKA LLLGVPSEAE LWDGTENIDP 401 LRYHQAREAL LDTLAVRFLP KWHELDRQAA KQENQSYEYS PETADWRTLR 451 NVCRAFVLRA DPAHIETVAE KYGEMAQNMT HEWGILSAVN GNESDTRNCL 501 LAQFADKFSD DALVMDKYFA LIGSSRRSDT LQQVQTALQH PKFSLENPNK 551 ARSLIGSFSR NVPHFHAQDG SGYRFIADKV IEIDRFNPQV AARLVQAFNL 601 CNKLEPHRKN LVKQELQCIR AQEGLSKDVG EIVGKILG* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2161>: m665. seq 1 ATGAAATGGG ACGAAACGCG CTTCGGTTTG GAATACGACT TGGATATTTT 51 CATGGTCGTC GCCGTGGGCG ATTTCAATAT GGGCGCGATG GAAAACAAGG 101 GTTTGAACAT CTTTAACACC AAGTTCGTCC TTGCCGACAG CCGCACCGCC 151 ACCGATACCG ATTTCGAAGG CATCGAATCC GTGGTCGGAC ACGAGTATTT 201 CCACAACTGG ACGGGCAACC GCGTAACCTG CCGCGACTGG TTCCAGCTTT 251 CGCTGAAGGA AGGGCTGACC GTGTTCCGCG ACCAAGAATT TTCCGGCGAC 301 CGCGCCAGCC GCGCCGTGCG CCGCATCGAA AACATCCGCC TGCTGCGCCA 351 GCACCAGTTC CCCGAAGACG CAGGCCCGAC CGCCCATCCG GTGCGCCCCG 401 CCAGCTATGA GGAGATGAAC AATTTCTACA CCATGACCGT TTATGAAAAA 451 GGCGCGGAAG TAGTGCGGAT GTATCACACC CTGCTCGGCG AAGAGGGCTT 501 CCAGAAAGGC ATGAAGCTCT ATTTCCAACG CCACGACGGA CAGGCCGTTA 551 CCTGCGACGA TTTCCGCGCG GCGATGGCGG ACGCGAACGG CATCAATCTC 601 GACCAGTTCG CCTTGTGGTA CAGCCAGGCG GGCACGCCCG TTTTGGAAGC 651 GGAAGGTCGT CTGAAAAACA ATATTTTCGA GTTGACCGTC AAACAAACCG 701 TGCCGCCCAC GCCCGATATG ACGGATAAAC AGCCGATGAT GATTCCCGTC 751 AAGGTCGGGC TGCTGAACCG CAACGGCGAA GCGGTGGCAT TCGACTATCA 801 GGGCAAACGC GCGACCGAAG CCGTGTTGCT GCTGACCGAA GCCGAACAGA 851 CCTTCCTGCT CGAAGGCGTA ACCGAAGCCG TCGTTCCCTC GCTGCTGCGC 901 GGGTTCAGCG CGCCGGTGCA TCTGAACTAT CCGTACAGCG ACGACGACCT 951 GCTGCTCCTG CTCGCCCATG ACAGCGACGC CTTCACGCGC TGGGAAGCCG 1001 CCCAAACGCT CTACCGCCGC GCCGTCGCCG CCAACCTTGC CACGCTTTCA 1051 GACGGCGTTG AGCTGCCGAA ACACGAAAAA CTGCTTGCCG CCGTCGAAAA 1101 AGTCATTTCA GACGACCTCT TAGACAACGC CTTCAAAGCC CTGCTTTTGG 1151 GCGTGCCATC CGAAGCCGAG CTGTGGGACG GCGCAGAAAA CATCGACCCG 1201 CTGCGCTACC ATCAGGCGCG CGAAGCCTTG TTGGATACGC TTGCCGTCCA 1251 CTTCCTGCCG AAATGGCACG AATTGAACCG TCAGGCGGCG AAGCAGGAAA 1301 ACCAAAGCTA CGAATACAGC CCCGAAGCCG CCGGCTGGCG CACGCTGCGC 1351 AACGTCTGCC GCGCCTTTGT CCTGCGCGCC GACCCCGCGC ACATCGAAAC 1401 CGTTGCCGAA AAATACGGCG AAATGGCGCA AAACATGACC CACGAATGGG 1451 GCATCCTGTC CGCCGTCAAC GGCAACGAAA GCGATACGCG CAACCGCCTG 1501 CTGGCGCAGT TTGCCGACAA GTTTTCAGAC GACGCGCTGG TGATGGACAA 1551 ATATTTTGCC CTCGTCGGCT CAAGCCGCCG CAGCGACACC CTGCAACAGG 1601 TTCGAACCGC CTTGCAGCAT CCGAAATTCA GCCTCGAAAA CCCCAACAAA 1651 GCCCGTTCGC TCATCGGCAG CTTCAGCCGC AACGTCCCGC ATTTCCACGC 1701 AGAAGACGGC AGCGGCTACC GCTTCATCGC CGACAAAGTC ATCGAAATCG 1751 ACCGCTTCAA CCCGCAGGTC GCCGCCCGCT TAGTGCAGGC GTTCAACCTC 1801 TGCAACAAGC TCGAGCCGCA CCGCAAAAAC TTGGTGAAAC AAGCATTGCA 1851 GCGCATTCGG GCGCAGGAAG GATTGTCGAA AGACGTGGGC GAAATCGTCG 1901 GCAAAATTTT GGATTGA This corresponds to the amino acid sequence <SEQ ID 2162; ORF 665>: m665.pep 1 MKWDETRFGL EYDLDIFMW AVGDFNMGAM ENKGLNIFNT KFVLADSRTA 51 TDTDFEGIES VVGHEYFHNW TGNRVTCRDW FQLSLKEGLT VFRDQEFSGD 101 RASRAVRRIE NIRLLRQHQF PEDAGPTAHP VRPASYEEMN NFYTMTVYEK 151 GAEWRMYHT LLGEEGFQKG MKLYFQRHDG QAVTCDDFRA AMADANGINL 201 DQFALWYSQA GTPVLEAEGR LKNNIFELTV KQTVPPTPDM TDKQPMMIPV 251 KVGLLNRNGE AVAFDYQGKR ATEAVLLLTE AEQTFLLEGV TEAWPSLLR 301 GFSAPVHLNY PYSDDDLLLL LAHDSDAFTR WEAAQTLYRR AVAANLATLS 351 DGVELPKHEK LLAAVEKVIS DDLLDNAFKA LLLGVPSEAE LWDGAENIDP 401 LRYHQAREAL LDTLAVHFLP KWHELNRQAA KQENQSYEYS PEAAGWRTLR 451 NVCRAFVLRA DPAHIETVAE KYGEMAQNMT HEWGILSAVN GNESDTRNRL 501 LAQFADKFSD DALVMDKYFA LVGSSRRSDT LQQVRTALQH PKFSLENPNK 551 ARSLIGSFSR NVPHFHAEDG SGYRFIADKV IEIDRFNPQV AARLVQAFNL 601 CNKLEPHRKN LVKQALQRIR AQEGLSKDVG EIVGKILD* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m665/g665 96.1% identity in 637 aa overlap 10 20 30 40 50 60 m665.pep MKWDETRFGLEYDLDIFMWAVGDFΝMGAMEΝKGLΝIFΝTKFVLADSRTATDTDFEGIES II I II II II II I II II II I I II II II I I II II II II I II II II II II II II II II II II I g665 MKWDETRFGLEYDLDIFMWAVGDFΝMGAMEΝKGLΝIFΝTKFVLADSRTATDTDFEGIES 10 20 30 40 50 60 70 80 90 100 110 120 m665. pep WGHEYFHΝWTGΝRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRAVRRIEΝIRLLRQHQF MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g665 VVGHEYFHΝWTGΝRVTCRDWFQLSLKEGLTVFRDQEFSGDRAGRAVRRIEΝIRLLRQΝQF 70 80 90 100 110 120 130 140 150 160 170 180 m665.pep PEDAGPTAHPVRPASYEEMΝΝFYTMTVYEKGAEWRMYHTLLGEEGFQKGMKLYFQRHDG MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g665 PEDAGPTAHPVRPVSYEEMΝΝFYTMTVYEKGAEWRMYHTLLGEEGFQKGMKLYFQRHDG 130 140 150 160 170 180 190 200 210 220 230 240 m665.pep QAVTCDDFRAAMADAΝGIΝLDQFALWYSQAGTPVLEAEGRLKΝΝIFELTVKQTVPPTPDM II II II I II II II I II I II II I II II II II I II I II I II II II I : II I I : II I II II II I g665 QAVTCDDFRAAMADAΝGIΝLDQFALWYSQAGTPVLEAEGRLKΝΝVFELTIKQTVPPTPDM 190 200 210 220 230 240 250 260 270 280 290 300 m665. pep TDKQPMMIPVKVGLLΝRΝGEAVAFDYQGKRATEAVLLLTEAEQTFLLEGVTEAVVPSLLR M I II I I II II II II II II I II II II I II II I II II MM I II: I II II II II II II II g665 ADK Q PMMIPVKVGLLΝRΝGEAVAFDY Q GKRATEAVLLMTEAEQAFPLEGVTEAWPSLLR 250 260 270 280 290 300 310 320 330 340 350 360 m665.pep GFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTLYRRAVAANLATLSDGVELPKHEK II II I I : II II II II II I II I II I I II II I II II II I II II II II I : II II : II II II g 665 GFSAPVYLNYPYSDDDLLLLLAHDSDAFTCWEAAQTLYRRAVAANLAALSDGIGLPKHEK 310 320 330 340 350 360 370 380 390 400 410 420 m665. pep LLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGAENIDPLRYHQAREALLDTLAVHFLP II II II II II II II II II II II II I II II II II I : II I I II II I II II II I II II I : II I g665 LLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGTENIDPLRYHQAREALLDTLAVRFLP 370 380 390 400 410 420 430 440 450 460 470 480 m665. pep KWHELNRQAAKQENQSYEYSPEAAGWRTLRNVCRAFVLRADPAHIETVAEKYGEMAQNMT M M M M M M M M M M M M I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g665 KWHELDRQAAKQENQSYEYSPETADWRTLRNVCRAFVLRADPAHIETVAEKYGEMAQNMT 430 440 450 460 470 480 490 500 510 520 530 540 m665. pep HEWGILSAVNGNESDTRNRLLAQFADKFSDDALVMDKYFALVGSSRRSDTLQQVRTALQH II II II II II I I II II II II II I II II II I II II II II II: II II II I II II I : I II II g665 HEWGILSAVNGNESDTRNCLLAQFADKFSDDALVMDKYFALIGSSRRSDTLQQVQTALQH 490 500 510 520 530 540 550 560 570 580 590 600 m665.pep PKFSLENPNKARSLIGSFSRNVPHFHAEDGSGYRFIADKVIEIDRFNPQVAARLVQAFNL MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g665 PKFSLENPNKARSLIGSFSRNVPHFHAQDGSGYRFIADKVIEIDRFNPQVAARLVQAFNL 550 560 570 580 590 600 610 620 630 639 m665. pep CNKLEPHRKNLVKQALQRIRAQEGLSKDVGEIVGKILDX I I II II II II II II II II II II II II I II II I I II g665 CNKLEPHRKNLVKQELQCIRAQEGLSKDVGEIVGKILGX 610 620 630 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2163>: a665. seq 1 ATGAAGTGGG ACGAAACGCG CTTCGGTTTG GAATACGACT TGGATATTTT 51 CATGGTCGTC GCCGTGGGCG ATTTCAATAT GGGTGCGATG GAAAACAAGG 101 GTTTGAACAT CTTTAACACC AAGTTCGTCC TTGCCGACAG CCGTACCGCC 151 ACCGATACCG ATTTTGAAGG CATCGAATCC GTGGTCGGAC ACGAATATTT 201 CCACAACTGG ACGGGCAACC GCGTGACCTG CCGCGACTGG TTCCAGCTTT 251 CGCTGAAGGA AGGGTTGACC GTGTTCCGCG ACCAAGAATT TTCCGGCGAC 301 CGCGCCAGCC GCGCCGTGCG CCGTATCGAA AACATCCGCC TGCTGCGCCA 351 GCACCAGTTC CCCGAAGACG CAGGTCCGAC CGCACATCCG GTGCGCCCCG 401 CCCGATATGA GGAGATGAAC AATTTCTACA CCATGACCGT TTATGAAAAA 451 GGCGCGGAAG TGGTGCGGAT GTATCACACC TTGCTCGGCG AAGAGGGCTT 501 CCAAAAAGGT ATGAAGCTCT ATTTCCAACG CCACGACGGA CAGGCTGTTA 551 CCTGCGACGA TTTCCGCGCG GCGATGGTGG ACGCGAACGG CATCAACCTC 601 GACCAATTCG CCTTGTGGTA CAGCCAAGCA GGTACGCCGG TTTTAGATGC 651 TCAAGGGCGT CTGAAAAACA ATGTGTTCGA GTTAACCATC AAACAAACCG 701 TGCCGCCCAC GCCCGATATG GCGGACAAAC AGCCGATGAT GATTCCCGTC 751 AAAATCGGGC TGCTGAACTG CAACGGCGAA GCGGTGGCAT TTGATTATCA 801 GGGCAAACGC GCGACCGAAG CCGTGTTGCT GCTGACCGAA GCCGAACAGA 851 CCTTCCAGTT CGAAAGCGTA ACCGAAGCCG TCGTTCCCTC GCTGCTGCGC 901 GGGTTCAGCG CGCCGGTGCA TCTGAACTAT CCGTACAGCG ACGACGACCT 951 GCTGCTTCTG CTCGCCCATG ACAGCGACGC CTTCACGCGC TGGGAAGCCG 1001 CACAAACGCT CTACCGCCGT GCCGTCGCCG CCAACCTTGC CGCGCTTTCA 1051 GACGGCGTCG AGTTGCCGAA ACACGAAAAA CTGCTTGCCG CCGTCGAAAA 1101 AGTCATTTCA GACGACCTCT TAGACAACGC TTTCAAAGCC CTGCTTTTGG 1151 GTGTGCCGTC TGAAGCCGAG CTGTGGGACG GCGCGGAAAA CATCGACCCG 1201 CTGCGCTACC ATCAGGCGCG CGAAGCCTTG TTGGATATAC TTGCCGTCCG 1251 CTTTCTGCCG AAATGGCACG AATTGAACCG TCAGGCGGCG AAGCAGGAAA 1301 ACCAAAGCTA CGAGTACAGC CCCGAAGCCG CCGGTTGGCG CACGCTGCGC 1351 AATGTCTGCC GCGCCTTCGT CCTGCGCGCC GATCCCGCGC ACATCGAAAC 1401 CGTTGCCGAG AAATACGCCG AAATGGCGCA AAACATGACC CACGAATGGG 1451 GCATCCTGTC CGCCGTCAAC GGCAACGAAA GCGATACGCG CAACCGCCTG 1501 CTGGCGCAGT TTGCCGACAA GTTTTCAGAC GACGCGCTGG TGATGGACAA 1551 ATATTTCGCC CTCGTCGGCT CAAGCCGCCG CAGCGACACC CTGCAACAGG 1601 TTCAAACCGC CTTGCAGCAT CCGAAGTTCA GCCTCGAAAA TCCCAACAAA 1651 GCCCGCTCGC TCATCGGCAG CTTCAGCCGC AACGTCCCGC ATTTCCACGC 1701 AGAAGACGGC AGCGGCTACC GCTTCATCGC CGACAAAGTC ATCGAAATCG 1751 ACCGCTTTAA CCCGCAGGTC GCCGCCCGCC TGGTGCAGGC GTTCAACCTC 1801 TGCAACAAGC TCGAGCCGCA CCGCAAAAAC TTGGTGAAAC AAGCATTGCA 1851 GCGCATTCGG GCGCAGGAAG GATTGTCGAA AGACGTGGGC GAAATCGTCG 1901 GCAAAATTTT GGATTGA This corresponds to the amino acid sequence <SEQ ID 2164; ORF 665. a>: a665.pep 1 MKWDETRFGL EYDLDIFMW AVGDFNMGAM ENKGLNIFNT KFVLADSRTA 51 TDTDFEGIES VVGHEYFHNW TGNRVTCRDW FQLSLKEGLT VFRDQEFSGD 101 RASRAVRRIE NIRLLRQHQF PEDAGPTAHP VRPARYEEMN NFYTMTVYEK 151 GAEWRMYHT LLGEEGFQKG MKLYFQRHDG QAVTCDDFRA AMVDANGINL 201 DQFALWYSQA GTPVLDAQGR LKNNVFELTI KQTVPPTPDM ADKQPMMIPV 251 KIGLLNCNGE AVAFDYQGKR ATEAVLLLTE AEQTFQFESV TEAWPSLLR 301 GFSAPVHLNY PYSDDDLLLL LAHDSDAFTR WEAAQTLYRR AVAANLAALS 351 DGVELPKHEK LLAAVEKVIS DDLLDNAFKA LLLGVPSEAE LWDGAENIDP 401 LRYHQAREAL LDILAVRFLP KWHELNRQAA KQENQSYEYS PEAAGWRTLR 451 NVCRAFVLRA DPAHIETVAE KYAEMAQNMT HEWGILSAVN GNESDTRNRL 501 LAQFADKFSD DALVMDKYFA LVGSSRRSDT LQQVQTALQH PKFSLENPNK 551 ARSLIGSFSR NVPHFHAEDG SGYRFIADKV IEIDRFNPQV AARLVQAFNL 601 CNKLEPHRKN LVKQALQRIR AQEGLSKDVG EIVGKILD* m665/a665 97.3% identity in 638 aa overlap 10 20 30 40 50 60 m665.pep MKWDETRFGLEYDLDIFMWAVGDFNMGAMENKGLNIFNTKFVLADSRTATDTDFEGIES I II II I II I II II I I II II I II II II II II II II II II I II II II I II II II II II II II a665 MKWDETRFGLEYDLDIFMWAVGDFNMGAMENKGLNIFNTKFVLADSRTATDTDFEGIES 10 20 30 40 50 60 70 80 90 100 110 120 m665.pep WGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRAVRRIENIRLLRQHQF I I II II II I II II II I II II II II II II II II II II II II II II I II I II II II II II II a665 WGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRAVRRIENIRLLRQHQF 70 80 90 100 110 120 130 140 150 160 170 180 m665. pep PEDAGPTAHPVRPAΞYEEMNNFYTMTVYEKGAEWRMYHTLLGEEGFQKGMKLYFQRHDG II II I I I I I II I I I I I I I I I I I I II I I I I I I I I I I II II II Ml II I I I I I I I I II I I I a665 PEDAGPTAHPVRPARYEEMNNFYTMTVYEKGAEWRMYHTLLGEEGFQKGMKLYFQRHDG 130 140 150 160 170 180 190 200 210 220 230 240 m665.pep QAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAEGRLKNNIFELTVKQTVPPTPDM II II I II II I I M II II II II II II II II II II I M M I II II M II I I: II II I II II I a665 QAVTCDDFRAAMVDANGINLDQFALWYSQAGTPVLDAQGRLKNNVFELTIKQTVPPTPDM 190 200 210 220 230 240 250 260 270 280 290 300 m665. pep TDKQPMMIPVKVGLLNRNGEAVAFDYQGKRATEAVLLLTEAEQTFLLEGVTEAWPSLLR : II II II II I M II II I I I II II II II II II II II I I I II II I I : I : II II II I I II I a665 ADKQPMMIPVKIGLLNCNGEAVAFDYQGKRATEAVLLLTEAEQTFQFESVTEAWPSLLR 250 260 270 280 290 300 310 320 330 340 350 360 m665.pep GFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTLYRRAVAANLATLSDGVELPKHEK II I II II II I I II II II II II I II II I II II I I II II I II II I II II : II I I I I I I II II a665 GFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTLYRRAVAANLAALSDGVELPKHEK 310 320 330 340 350 360 370 380 390 400 410 420 m665. pep LLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGAENIDPLRYHQAREALLDTLAVHFLP II II II II II I II II I II II II II II II II II II I I I I II II II II II II II II I : I I I a665 LLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGAENIDPLRYHQAREALLDILAVRFLP 370 380 390 400 410 420 430 440 450 460 470 480 m665. pep KWHELNRQAAKQENQSYEYSPEAAGWRTLRNVCRAFVLRADPAHIETVAEKYGEMAQNMT II II II I II II II II I II II II II II II II II II II II II II II II II II II : II II II I a665 KWHELNRQAAKQENQSYEYSPEAAGWRTLRNVCRAFVLRADPAHIETVAEKYAEMAQNMT 430 440 450 460 470 480 490 500 510 520 530 540 m665.pep HEWGILΞAVNGNESDTRNRLLAQFADKFSDDALVMDKYFALVGSSRRSDTLQQVRTALQH II II II II II II I II I II II II I II II II I II II II I I II II II II II II II I I : II II I a665 HEWGILSAVNGNESDTRNRLLAQFADKFSDDALVMDKYFALVGSSRRSDTLQQVQTALQH 490 500 510 520 530 540 550 560 570 580 590 600 m665.pep PKFSLENPNKARSLIGSFSRNVPHFHAEDGSGYRFIADKVIEIDRFNPQVAARLVQAFNL I II II II II II II II II II II II II II II II I I II II I I II II I I II II II I I II II I II a665 PKFSLENPNKARSLIGSFSRNVPHFHAEDGSGYRFIADKVIEIDRFNPQVAARLVQAFNL 550 560 570 580 590 600 610 620 630 639 m665.pep CNKLEPHRKNLVKQALQRIRAQEGLSKDVGEIVGKILDX I II II II II II II II II I I I II II I II II II II I II II I a665 CNKLEPHRKNLVKQALQRIRAQEGLSKDVGEIVGKILDX 610 620 630 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2165>: g665-l.seq 1 ATGAGCAAAA CCGTCCGTTA TCTGAAAGAT TACCAAACGC CTGCCTACCG 51 CATTCTTGAA ACCGAACTGC ATTTCGACAT TGCCGAACCG CAAACCGTCG 101 TGAAGTCGCG TTTGACGGTC GAGCCGCAGA GGGCGGGCGA GCCGCTGGTG 151 TTGGACGGTT CGGCAAAACT CTTGTCCGTC AAAATCAACG GCGCGGCGGC 201 GGATTATGTG TTGGAAGGCG AGACGCTGAC GATTGCAGAC GTACCGTCCG 251 AACGCTTCAC CGTCGAAGTG GAAACCGAAA TCCTGCCGGC GGAAAACAAA 301 TCGCTGATGG GGCTGTATGC TTCCGGCGGC AATCTGTTTA CCCAGTGCGA 351 GCCGGAGGGC TTCCGCAAAA TCACGTTCTA CATCGACCGT CCGGATGTGA 401 TGTCCAAGTT CACGACCACC ATCGTCGCGG ACAAAAAACG CTATCCCGTT 451 TTGCTTTCCA ACGGCAACAA AATCGACGGC GGCGAGTTTT CAGACGGCCG 501 CCATTGGGTG AAATGGGAAG ACCCGTTTGC CAAACCGAGT TATCTGTTTG 551 CTTTGGTCGC GGGCGATTTG GCGGTAACGG AAGACCGTTT CACCACCATG 601 AGCGGCAGAA ACGTCAAAAT CGAGTTTTAC ACCACCGAAG CGGACAAACC 651 CAAGGTCGGC TTTGCCGTGG AATCGTTGAA AAACGCGATG AAGTGGGACG 701 AAACGCGCTT CGGGTTGGAA TATGACTTGG ATATTTTCAT GGTCGTCGCC 751 GTAGGCGATT TCAATATGGG CGCGATGGAA AACAAGGGTT TGAACATTTT 801 TAACACCAAG TTCGTCCTCG CCGACAGCCG CACCGCCACC GATACCGATT 851 TCGAAGGCAT TGAATCCGTG GTCGGACACG AATATTTCCA CAACTGGACG 901 GGCAACCGCG TAACCTGCCG CGACTGGTTC CAGCTTTCGC TGAAGGAAGG 951 GCTGACCGTG TTCCGCGACC AAGAGTTTTC CGGCGACCGC GCCGGCCGCG 1001 CCGTGCGCCG CATCGAGAAC ATCCGCCTGC TGCGCCAGAA CCAGTTCCCC 1051 GAAGACGCAG GCCCGACCGC CCATCCGGTG CGCCCCGTCA GCTATGAGGA 1101 GATGAACAAT TTCTACACCA TGACCGTTTA TGAAAAAGGC GCGGAAGTGG 1151 TGCGGATGTA TCATACCCTG CTCGGCGAAG AGGGCTTCCA AAAAGGCATG 1201 AAGCTATATT TCCAACGCCA CGACGGACAG GCAGTGACCT GCGACGATTT 1251 CCGCGCGGCG ATGGCGGATG CGAACGGCAT CAATCTCGAC CAGTTCGCCT 1301 TGTGGTACAG CCAGGCGGGC ACGCCCGTTT TGGAAGCCGA AGGCCGTCTG 1351 AAAAACAATG TTTTCGAGTT AACCATTAAA CAAACCGTGC CGCCCACGCC 1401 CGATATGGCG GACAAACAGC CGATGATGAT TCCCGTCAAA GTCGGGCTTC 1451 TGAACCGCAA CGGCGAAGCG GTGGCATTCG ATTATCAGGG CAAACGCGCA 1501 ACCGAAGCCG TGTTGCTGAT GACCGAAGCC GAACAGGCCT TCCCGCTCGA 1551 AGGTGTAACC GAAGCCGTCG TTCCCTCGCT GCTGCGCGGG TTCAGCGCGC 1601 CAGTGTATCT GAACTATCCG TACAGCGACG ACGACCTGCT GCTCCTGCTC 1651 GCCCACGACA GCGACGCTTT CACGTGCTGG GAAGCCGCCC AAACGCTCTA 1701 CCGTCGCGCC GTCGCCGCCA ACCTTGCCGC GCTTTCAGAC GGCATCGGGT 1751 TGCCGAAACA CGAAAAACTG CTTGCCGCCG TCGAAAAAGT CATTTCAGAC 1801 GACCTCTTGG ACAACGCCTT CAAAGCCCTG CTTTTGGGCG TGCCGTCCGA 1851 AGCCGAACTG TGGGACGGCA CGGAAAACAT CGACCCGCTG CGCTACCATC 1901 AGGCGCGCGA AGCCTTGTTG GATACGCTTG CCGTCCGCTT CCTGCCGAAA 1951 TGGCACGAAT TGGACCGTCA GGCGGCGAAG CAGGAAAACC AAAGTTACGA 2001 ATACAGCCCC GAAACCGCCG ACTGGCGCAC GCTGCGCAAC GTCTGCCGCG 2051 CCTTCGTCCT GCGCGCCGAC CCCGCGCACA TCGAAACTGT TGCCGAAAAA 2101 TACGGCGAAA TGGCGCAAAA CATGACCCAC GAATGGGGCA TCCTGTCCGC 2151 CGTCAACGGC AACGAAAGCG ATACGCGCAA CTGCCTGCTG GCGCAGTTTG 2201 CCGACAAGTT TTCAGACGAC GCGCTGGTGA TGGACAAATA TTTCGCCCTT 2251 ATCGGCTCAA GCCGCCGCAG CGACACCCTG CAACAGGTTC AAACCGCCTT 2301 GCAGCATCCG AAATTCAGTC TCGAAAACCC CAACAAAGCC CGTTCGCTCA 2351 TCGGCAGCTT CAGCCGCAAC GTCCCGCATT TTCACGCACA AGACGGCAGC 2401 GGCTACCGCT TCATCGCCGA CAAAGTCATC GAAATCGACC GCTTCAACCC 2451 GCAGGTCGCC GCCCGCCTGG TGCAGGCGTT CAACCTCTGC AACAAGCTCG 2501 AGCCGCACCG CAAAAACTTG GTGAAACAAG AATTGCAGTG CATTCGGGCG 2551 CAGGAAGGAT TGTCGAAAGA CGTGGGCGAA ATCGTCGGCA AGATTTTGGG 2601 TTGA This corresponds to the amino acid sequence <SEQ ID 2166; ORF 665-l.ng>: g665-l.pep 1 MSKTVRYLKD YQTPAYRILE TELHFDIAEP QTVVKSRLTV EPQRAGEPLV 51 LDGSAKLLSV KINGAAADYV LEGETLTIAD VPSERFTVEV ETEILPAENK 101 SLMGLYASGG NLFTQCEPEG FRKITFYIDR PDVMSKFTTT IVADKKRYPV 151 LLSNGNKIDG GEFSDGRHWV KWEDPFAKPS YLFALVAGDL AVTEDRFTTM 201 SGRNVKIEFY TTEADKPKVG FAVESLKNAM KWDETRFGLE YDLDIFMVVA 251 VGDFNMGAME NKGLNIFNTK FVLADSRTAT DTDFEGIESV VGHEYFHNWT 301 GNRVTCRDWF QLSLKEGLTV FRDQEFSGDR AGRAVRRIEN IRLLRQNQFP 351 EDAGPTAHPV RPVΞYEEMNN FYTMTVYEKG AEVVRMYHTL LGEEGFQKGM 401 KLYFQRHDGQ AVTCDDFRAA MADANGINLD QFALWYSQAG TPVLEAEGRL 451 KNNVFELTIK QTVPPTPDMA DKQPMMIPVK VGLLNRNGEA VAFDYQGKRA 501 TEAVLLMTEA EQAFPLEGVT EAVVPSLLRG FSAPVYLNYP YSDDDLLLLL 551 AHDSDAFTCW EAAQTLYRRA VAANLAALSD GIGLPKHEKL LAAVEKVISD 601 DLLDNAFKAL LLGVPSEAEL WDGTENIDPL RYHQAREALL DTLAVRFLPK 651 WHELDRQAAK QENQSYEYΞP ETADWRTLRN VCRAFVLRAD PAHIETVAEK 701 YGEMAQNMTH EWGILSAVNG NEΞDTRNCLL AQFADKFSDD ALVMDKYFAL 751 IGSSRRΞDTL QQVQTALQHP KFSLENPNKA RSLIGΞFΞRN VPHFHAQDGS 801 GYRFIADKVI EIDRFNPQVA ARLVQAFNLC NKLEPHRKNL VKQELQCIRA 851 QEGLSKDVGE IVGKILG* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2167>: 1 ATGAGCAAAA CCGTGCATTA TCTCAAAGAC TATCAAACGC CCGCCTACCA 51 TATTCTCAAA ACCGATTTAC ATTTTGATAT TAATGAACCG CAAACCGTCG 101 TGAAGTCGCG TTTGACGGTT GAGCCGCAGA GGGTAGGGGA GCCGCTGGTG 151 TTGGACGGTT CGGCGAAACT CTTGTCCGTC AAAATCAACG GGGCGGCGGC 201 GGATTATGTG TTGGAAGGAG AGACGCTGAC GATTGCGGGC GTGCCGTCCG 251 AACGCTTCAC CGTCGAAGTG GAAACCGAAA TCCTGCCGGC GGAAAACAAA 301 TCGCTGATGG GGCTGTATGC TTCCGGCGGC AATTTGTTTA CCCAGTGCGA 351 GCCGGAGGGC TTCCGCAAAA TCACATTTTA CATCGACCGT CCGGATGTGA 401 TGTCCAAGTT CACCACCACC ATCGTCGCCG ACAAAAAACG CTATCCCGTT 451 TTGCTTTCCA ACGGCAACAA AATCGACGGC GGCGAGTTTT CAGACGGCCG 501 CCATTGGGTG AAATGGGAAG ACCCGTTTTC CAAACCGAGC TATCTGTTTG 551 CTTTGGTCGC GGGCGATTTG GCGGTAACGG AAGACTATTT CACCACCATG 601 AGCGGCAGAA ACGTCAAAAT CGAGTTTTAC ACCACCGAAG CGGACAAGCC 651 CAAGGTCGGC TTTGCCGTGG AATCGTTGAA AAACGCGATG AAATGGGACG 701 AAACGCGCTT CGGTTTGGAA TACGACTTGG ATATTTTCAT GGTCGTCGCC 751 GTGGGCGATT TCAATATGGG CGCGATGGAA AACAAGGGTT TGAACATCTT 801 TAACACCAAG TTCGTCCTTG CCGACAGCCG CACCGCCACC GATACCGATT 851 TCGAAGGCAT CGAATCCGTG GTCGGACACG AGTATTTCCA CAACTGGACG 901 GGCAACCGCG TAACCTGCCG CGACTGGTTC CAGCTTTCGC TGAAGGAAGG 951 GCTGACCGTG TTCCGCGACC AAGAATTTTC CGGCGACCGC GCCAGCCGCG 1001 CCGTGCGCCG CATCGAAAAC ATCCGCCTGC TGCGCCAGCA CCAGTTCCCC 1051 GAAGACGCAG GCCCGACCGC CCATCCGGTG CGCCCCGCCA GCTATGAGGA 1101 GATGAACAAT TTCTACACCA TGACCGTTTA TGAAAAAGGC GCGGAAGTAG 1151 TGCGGATGTA TCACACCCTG CTCGGCGAAG AGGGCTTCCA GAAAGGCATG 1201 AAGCTCTATT TCCAACGCCA CGACGGACAG GCCGTTACCT GCGACGATTT 1251 CCGCGCGGCG ATGGCGGACG CGAACGGCAT CAATCTCGAC CAGTTCGCCT 1301 TGTGGTACAG CCAGGCGGGC ACGCCCGTTT TGGAAGCGGA AGGTCGTCTG 1351 AAAAACAATA TTTTCGAGTT GACCGTCAAA CAAACCGTGC CGCCCACGCC 1401 CGATATGACG GATAAACAGC CGATGATGAT TCCCGTCAAG GTCGGGCTGC 1451 TGAACCGCAA CGGCGAAGCG GTGGCATTCG ACTATCAGGG CAAACGCGCG 1501 ACCGAAGCCG TGTTGCTGCT GACCGAAGCC GAACAGACCT TCCTGCTCGA 1551 AGGCGTAACC GAAGCCGTCG TTCCCTCGCT GCTGCGCGGG TTCAGCGCGC 1601 CGGTGCATCT GAACTATCCG TACAGCGACG ACGACCTGCT GCTCCTGCTC 1651 GCCCATGACA GCGACGCCTT CACGCGCTGG GAAGCCGCCC AAACGCTCTA 1701 CCGCCGCGCC GTCGCCGCCA ACCTTGCCAC GCTTTCAGAC GGCGTTGAGC 1751 TGCCGAAACA CGAAAAACTG CTTGCCGCCG TCGAAAAAGT CATTTCAGAC 1801 GACCTCTTAG ACAACGCCTT CAAAGCCCTG CTTTTGGGCG TGCCATCCGA 1851 AGCCGAGCTG TGGGACGGCG CAGAAAACAT CGACCCGCTG CGCTACCATC 1901 AGGCGCGCGA AGCCTTGTTG GATACGCTTG CCGTCCACTT CCTGCCGAAA 1951 TGGCACGAAT TGAACCGTCA GGCGGCGAAG CAGGAAAACC AAAGCTACGA 2001 ATACAGCCCC GAAGCCGCCG GCTGGCGCAC GCTGCGCAAC GTCTGCCGCG 2051 CCTTTGTCCT GCGCGCCGAC CCCGCGCACA TCGAAACCGT TGCCGAAAAA 2101 TACGGCGAAA TGGCGCAAAA CATGACCCAC GAATGGGGCA TCCTGTCCGC 2151 CGTCAACGGC AACGAAAGCG ATACGCGCAA CCGCCTGCTG GCGCAGTTTG 2201 CCGACAAGTT TTCAGACGAC GCGCTGGTGA TGGACAAATA TTTTGCCCTC 2251 GTCGGCTCAA GCCGCCGCAG CGACACCCTG CAACAGGTTC GAACCGCCTT 2301 GCAGCATCCG AAATTCAGCC TCGAAAACCC CAACAAAGCC CGTTCGCTCA 2351 TCGGCAGCTT CAGCCGCAAC GTCCCGCATT TCCACGCAGA AGACGGCAGC 2401 GGCTACCGCT TCATCGCCGA CAAAGTCATC GAAATCGACC GCTTCAACCC 2451 GCAGGTCGCC GCCCGCTTAG TGCAGGCGTT CAACCTCTGC AACAAGCTCG 2501 AGCCGCACCG CAAAAACTTG GTGAAACAAG CATTGCAGCG CATTCGGGCG 2551 CAGGAAGGAT TGTCGAAAGA CGTGGGCGAA ATCGTCGGCA AAATTTTGGA 2601 TTGA This corresponds to the amino acid sequence <SEQ ID 2168; OI m665-l.pep 1 MSKTVHYLKD YQTPAYHILK TDLHFDINEP QTVVKΞRLTV EPQRVGEPLV 51 LDGSAKLLSV KINGAAADYV LEGETLTIAG VPSERFTVEV ETEILPAENK 101 SLMGLYASGG NLFTQCEPEG FRKITFYIDR PDVMSKFTTT IVADKKRYPV 151 LLΞNGNKIDG GEFSDGRHWV KWEDPFSKPS YLFALVAGDL AVTEDYFTTM 201 SGRNVKIEFY TTEADKPKVG FAVESLKNAM KWDETRFGLE YDLDIFMVVA 251 VGDFNMGAME NKGLNIFNTK FVLADSRTAT DTDFEGIEΞV VGHEYFHNWT 301 GNRVTCRDWF QLSLKEGLTV FRDQEFSGDR ASRAVRRIEN IRLLRQHQFP 351 EDAGPTAHPV RPASYEEMNN FYTMTVYEKG AEVVRMYHTL LGEEGFQKGM 401 KLYFQRHDGQ AVTCDDFRAA MADANGINLD QFALWYSQAG TPVLEAEGRL 451 KNNIFELTVK QTVPPTPDMT DKQPMMIPVK VGLLNRNGEA VAFDYQGKRA 501 TEAVLLLTEA EQTFLLEGVT EAVVPΞLLRG FSAPVHLNYP YSDDDLLLLL 551 AHDSDAFTRW EAAQTLYRRA VAANLATLΞD GVELPKHEKL LAAVEKVISD 601 DLLDNAFKAL LLGVPSEAEL WDGAENIDPL RYHQAREALL DTLAVHFLPK 651 WHELNRQAAK QENQSYEYSP EAAGWRTLRN VCRAFVLRAD PAHIETVAEK 701 YGEMAQNMTH EWGILΞAVNG NEΞDTRNRLL AQFADKFSDD ALVMDKYFAL 751 VGSSRRΞDTL QQVRTALQHP KFSLENPNKA RΞLIGSFSRN VPHFHAEDGS 801 GYRFIADKVI EIDRFNPQVA ARLVQAFNLC NKLEPHRKNL VKQALQRIRA 851 QEGLSKDVGE IVGKILD* 665-l/g665-l 96.1% identity in 866 aa overlap 10 20 30 40 50 60 m665-l.pep MSKTVHYLKDYQTPAYHILKTDLHFDINEPQTVVKSRLTVEPQRVGEPLVLDGSAKLLSV I I I I I : I I I I I I I I I Ml MM I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I g665-l MSKTVRYLKDYQTPAYRILETELHFDIAEPQTVVKSRLTVEPQRAGEPLVLDGSAKLLSV 10 20 30 40 50 60 70 80 90 100 110 120 m665-l.pep KINGAAADYVLEGETLTIAGVPSERFTVEVETEILPAENKSLMGLYASGGNLFTQCEPEG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g665-l KINGAAADYVLEGETLTIADVPSERFTVEVETEILPAENKSLMGLYASGGNLFTQCEPEG 70 80 90 100 110 120 130 140 150 160 170 180 m665-l.pep FRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDGGEFSDGRHWVKWEDPFSKPS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M I I g665-l FRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDGGEFSDGRHWVKWEDPFAKPS 130 140 150 160 170 180 190 200 210 220 230 240 m665-l . pep YLFALVAGDLAVTEDYFTTMΞGRNVKIEFYTTEADKPKVGFAVESLKNAMKWDETRFGLE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g665-l YLFALVAGDLAVTEDRFTTMSGRNVKIEFYTTEADKPKVGFAVESLKNAMKWDETRFGLE 190 200 210 220 230 240 250 260 270 280 290 300 m665-1. pep YDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADSRTATDTDFEGIESVVGHEYFHNWT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g665-l YDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADSRTATDTDFEGIESVVGHEYFHNWT 250 260 270 280 290 300 310 320 330 340 350 360 m665-l.pep GNRVTCRDWFQLΞLKEGLTVFRDQEFSGDRASRAVRRIENIRLLRQHQFPEDAGPTAHPV I I I I I I I I I I I I I I I II I I I I I I I I I I I I I Ml I I I I II I I I I I I Mil I I I I I I I I I I I g665-l GNRVTCRDWFQLSLKEGLTVFRDQEFΞGDRAGRAVRRIENIRLLRQNQFPEDAGPTAHPV 310 320 330 340 350 360 370 380 390 400 410 420 m665-l . pep RPASYEEMNNFYTMTVYEKGAEVVRMYHTLLGEEGFQKGMKLYFQRHDGQAVTCDDFRAA I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g665-l RPVΞYEEMNNFYTMTVYEKGAEVVRMYHTLLGEEGFQKGMKLYFQRHDGQAVTCDDFRAA 370 380 390 400 410 420 430 440 450 460 470 480 m665-l.pep MADANGINLDQFALWYSQAGTPVLEAEGRLKNNIFELTVKQTVPPTPDMTDKQPMMIPVK I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I : I I I I : I I I I I I I I I I : I I I I I I I I I I g665-l MADANGINLDQFALWYSQAGTPVLEAEGRLKNNVFELTIKQTVPPTPDMADKQPMMIPVK 430 440 450 460 470 480 490 500 510 520 530 540 m665-l.pep VGLLNRNGEAVAFDYQGKRATEAVLLLTEAEQTFLLEGVTEAVVPSLLRGFSAPVHLNYP I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I : I I I I I I I I I I I I I I I I I I I I I : I I I I g665-l VGLLNRNGEAVAFDYQGKRATEAVLLMTEAEQAFPLEGVTEAVVPSLLRGFSAPVYLNYP 490 500 510 520 530 540 550 560 570 580 590 600 m665-l.pep YSDDDLLLLLAHDSDAFTRWEAAQTLYRRAVAANLATLSDGVELPKHEKLLAAVEKVISD I I I I II I I I I I I I II I II I I I I I I I I I I I I I II IMM I I : lll lll g665-l YSDDDLLLLLAHDSDAFTCWEAAQTLYRRAVAANLAALSDGIGLPKHEKLLAAVEKVISD 550 560 570 580 590 600 610 620 630 640 650 660 m665-l . pep DLLDNAFKALLLGVPSEAELWDGAENIDPLRYHQAREALLDTLAVHFLPKWHELNRQAAK I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I :l I I I I g665-l DLLDNAFKALLLGVPSEAELWDGTENIDPLRYHQAREALLDTLAVRFLPKWHELDRQAAK 610 620 630 640 650 660 670 680 690 700 710 720 m665-l.pep QENQSYEYSPEAAGWRTLRNVCRAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNG I I I I I I I I I I l I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g665-l QENQSYEYSPETADWRTLRNVCRAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNG 670 680 690 700 710 720 730 740 750 760 770 780 m665-l.pep NESDTRNRLLAQFADKFSDDALVMDKYFALVGSSRRSDTLQQVRTALQHPKFSLENPNKA I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I :| I I I I I I I I I I I I I I I g665-l NESDTRNCLLAQFADKFΞDDALVMDKYFALIGSSRRSDTLQQVQTALQHPKFSLENPNKA 730 740 750 760 770 780 790 800 810 820 830 840 m665-l.pep RΞLIGΞFSRNVPHFHAEDGSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNL I I I I I I I I I I I I I I I I :l I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g665-l RΞLIGSFSRNVPHFHAQDGSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNL 790 800 810 820 830 840 850 860 m665-l.pep VKQALQRIRAQEGLSKDVGEIVGKILDX III II I I I I I I I I I I I I I I I I I I I g665-l VKQELQCIRAQEGLSKDVGEIVGKILGX 850 860 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2169>: a665-l.seq 1 ATGAGCAAAA CCGTGCATTA TCTCAAAGAC TATCAAACGC CCGCCTACCA 51 TATTCTCAAA ACCGATTTAC ATTTTGATAT TAACGAACCG CAAACCATTG 101 TGAAGTCGCG TTTGACGGTC GAGCCGAAGA GGGTGGGAGA GCCGCTGGTG 151 TTGGACGGTT CGGCGAAACT CTTGTCCGTC AAAATCAACG GCGTGGCGGC 201 GGATTATGTG TTGGAAGGCG AGACGCTGAC GATTGCGGAC GTGCCGTCCG 251 AACGCTTCAC CGTCGAAGTG GAAACCGAAA TCCTGCCGGC GGAAAACAAA 301 TCGCTGATGG GGCTGTATGC GTCCGCCGGT AACCTGTTTA CCCAGTGCGA 351 GCCGGAGGGC TTCCGCAAAA TCACGTTCTA TATCGACCGT CCGGATGTCA 401 TGTCCAAGTT CACGACCACC ATCGTCGCGG ACAAAAAACG CTATCCCGTT 451 TTGCTCTCCA ACGGCAACAA AATCGACGGC GGCGAGTATT CAGACGGCCG 501 CCATTGGGTG AAATGGGAAG ACCCGTTTGC CAAACCGAGT TATCTGTTTG 551 CTTTGGTCGC GGGCGATTTG GCGGTCACGG AAGACTATTT CACCACCATG 601 AGCGGCAGAA ACGTCAAAAT CGAGTTTTAC ACCACCGAAG CGGACAAGCC 651 CAAGGTCGGC TTTGCCGTGG AATCGCTGAA AAACGCAATG AAGTGGGACG 701 AAACGCGCTT CGGTTTGGAA TACGACTTGG ATATTTTCAT GGTCGTCGCC 751 GTGGGCGATT TCAATATGGG TGCGATGGAA AACAAGGGTT TGAACATCTT 801 TAACACCAAG TTCGTCCTTG CCGACAGCCG TACCGCCACC GATACCGATT 851 TTGAAGGCAT CGAATCCGTG GTCGGACACG AATATTTCCA CAACTGGACG 901 GGCAACCGCG TGACCTGCCG CGACTGGTTC CAGCTTTCGC TGAAGGAAGG 951 GTTGACCGTG TTCCGCGACC AAGAATTTTC CGGCGACCGC GCCAGCCGCG 1001 CCGTGCGCCG TATCGAAAAC ATCCGCCTGC TGCGCCAGCA CCAGTTCCCC 1051 GAAGACGCAG GTCCGACCGC ACATCCGGTG CGCCCCGCCC GATATGAGGA 1101 GATGAACAAT TTCTACACCA TGACCGTTTA TGAAAAAGGC GCGGAAGTGG 1151 TGCGGATGTA TCACACCTTG CTCGGCGAAG AGGGCTTCCA AAAAGGTATG 1201 AAGCTCTATT TCCAACGCCA CGACGGACAG GCTGTTACCT GCGACGATTT 1251 CCGCGCGGCG ATGGTGGACG CGAACGGCAT CAACCTCGAC CAATTCGCCT 1301 TGTGGTACAG CCAAGCAGGT ACGCCGGTTT TAGATGCTCA AGGGCGTCTG 1351 AAAAACAATG TGTTCGAGTT AACCATCAAA CAAACCGTGC CGCCCACGCC 1401 CGATATGGCG GACAAACAGC CGATGATGAT TCCCGTCAAA ATCGGGCTGC 1451 TGAACTGCAA CGGCGAAGCG GTGGCATTTG ATTATCAGGG CAAACGCGCG 1501 ACCGAAGCCG TGTTGCTGCT GACCGAAGCC GAACAGACCT TCCAGTTCGA 1551 AAGCGTAACC GAAGCCGTCG TTCCCTCGCT GCTGCGCGGG TTCAGCGCGC 1601 CGGTGCATCT GAACTATCCG TACAGCGACG ACGACCTGCT GCTTCTGCTC 1651 GCCCATGACA GCGACGCCTT CACGCGCTGG GAAGCCGCAC AAACGCTCTA 1701 CCGCCGTGCC GTCGCCGCCA ACCTTGCCGC GCTTTCAGAC GGCGTCGAGT 1751 TGCCGAAACA CGAAAAACTG CTTGCCGCCG TCGAAAAAGT CATTTCAGAC 1801 GACCTCTTAG ACAACGCTTT CAAAGCCCTG CTTTTGGGTG TGCCGTCTGA 1851 AGCCGAGCTG TGGGACGGCG CGGAAAACAT CGACCCGCTG CGCTACCATC 1901 AGGCGCGCGA AGCCTTGTTG GATATACTTG CCGTCCGCTT TCTGCCGAAA 1951 TGGCACGAAT TGAACCGTCA GGCGGCGAAG CAGGAAAACC AAAGCTACGA 2001 GTACAGCCCC GAAGCCGCCG GTTGGCGCAC GCTGCGCAAT GTCTGCCGCG 2051 CCTTCGTCCT GCGCGCCGAT CCCGCGCACA TCGAAACCGT TGCCGAGAAA 2101 TACGCCGAAA TGGCGCAAAA CATGACCCAC GAATGGGGCA TCCTGTCCGC 2151 CGTCAACGGC AACGAAAGCG ATACGCGCAA CCGCCTGCTG GCGCAGTTTG 2201 CCGACAAGTT TTCAGACGAC GCGCTGGTGA TGGACAAATA TTTCGCCCTC 2251 GTCGGCTCAA GCCGCCGCAG CGACACCCTG CAACAGGTTC AAACCGCCTT 2301 GCAGCATCCG AAGTTCAGCC TCGAAAATCC CAACAAAGCC CGCTCGCTCA 2351 TCGGCAGCTT CAGCCGCAAC GTCCCGCATT TCCACGCAGA AGACGGCAGC 2401 GGCTACCGCT TCATCGCCGA CAAAGTCATC GAAATCGACC GCTTTAACCC 2451 GCAGGTCGCC GCCCGCCTGG TGCAGGCGTT CAACCTCTGC AACAAGCTCG 2501 AGCCGCACCG CAAAAACTTG GTGAAACAAG CATTGCAGCG CATTCGGGCG 2551 CAGGAAGGAT TGTCGAAAGA CGTGGGCGAA ATCGTCGGCA AAATTTTGGA 2601 TTGA This corresponds to the amino acid sequence <SEQ ID 2170; ORF 665-l.a>: a665-l.pep 1 MSKTVHYLKD YQTPAYHILK TDLHFDINEP QTIVKSRLTV EPKRVGEPLV 51 LDGSAKLLSV KINGVAADYV LEGETLTIAD VPΞERFTVEV ETEILPAENK 101 ΞLMGLYASAG NLFTQCEPEG FRKITFYIDR PDVMSKFTTT IVADKKRYPV 151 LLΞNGNKIDG GEYSDGRHWV KWEDPFAKPS YLFALVAGDL AVTEDYFTTM 201 SGRNVKIEFY TTEADKPKVG FAVESLKNAM KWDETRFGLE YDLDIFMVVA 251 VGDFNMGAME NKGLNIFNTK FVLADSRTAT DTDFEGIESV VGHEYFHNWT 301 GNRVTCRDWF QLΞLKEGLTV FRDQEFSGDR AΞRAVRRIEN IRLLRQHQFP 351 EDAGPTAHPV RPARYEEMNN FYTMTVYEKG AEVVRMYHTL LGEEGFQKGM 401 KLYFQRHDGQ AVTCDDFRAA MVDANGINLD QFALWYSQAG TPVLDAQGRL 451 KNNVFELTIK QTVPPTPDMA DKQPMMIPVK IGLLNCNGEA VAFDYQGKRA 501 TEAVLLLTEA EQTFQFEΞVT EAVVPSLLRG FSAPVHLNYP YSDDDLLLLL 551 AHDSDAFTRW EAAQTLYRRA VAANLAALSD GVELPKHEKL LAAVEKVISD 601 DLLDNAFKAL LLGVPΞEAEL WDGAENIDPL RYHQAREALL DILAVRFLPK 651 WHELNRQAAK QENQΞYEYSP EAAGWRTLRN VCRAFVLRAD PAHIETVAEK 701 YAEMAQNMTH EWGILSAVNG NESDTRNRLL AQFADKFSDD ALVMDKYFAL 751 VGSSRRSDTL QQVQTALQHP KFSLENPNKA RΞLIGSFΞRN VPHFHAEDGS 801 GYRFIADKVI EIDRFNPQVA ARLVQAFNLC NKLEPHRKNL VKQALQRIRA 851 QEGLSKDVGE IVGKILD* a665-l/m665-l 97.2% identity in 867 aa overlap 10 20 30 40 50 60 a665-l.pep MSKTVHYLKDYQTPAYHILKTDLHFDINEPQTIVKSRLTVEPKRVGEPLVLDGSAKLLSV II I III I I I I I I I II I I I I I I I I I I I I I I I I l I I I I I I I l I I I I I I II I I I I I II I m665-l MSKTVHYLKDYQTPAYHILKTDLHFDINEPQTVVKSRLTVEPQRVGEPLVLDGSAKLLSV 10 20 30 40 50 60 70 80 90 100 110 120 a665-l.pep KINGVAADYVLEGETLTIADVPSERFTVEVETEILPAENKSLMGLYASAGNLFTQCEPEG I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I m665-l KINGAAADYVLEGETLTIAGVPSERFTVEVETEILPAENKSLMGLYAΞGGNLFTQCEPEG 70 80 90 100 110 120 130 140 150 160 170 180 a665-l.pep FRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDGGEYSDGRHWVKWEDPFAKPS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M I I I I I I I I I I I I : I I I m665-l FRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDGGEFSDGRHWVKWEDPFSKPS 130 140 150 160 170 180 190 200 210 220 230 240 a665-l.pep YLFALVAGDLAVTEDYFTTMSGRNVKIEFYTTEADKPKVGFAVESLKNAMKWDETRFGLE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m665-l YLFALVAGDLAVTEDYFTTMSGRNVKIEFYTTEADKPKVGFAVESLKNAMKWDETRFGLE 190 200 210 220 230 240 250 260 270 280 290 300 a665-l.pep YDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADΞRTATDTDFEGIESVVGHEYFHNWT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m665-l YDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADSRTATDTDFEGIESVVGHEYFHNWT 250 260 270 280 290 300 310 320 330 340 350 360 a665-l.pep GNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRAVRRIENIRLLRQHQFPEDAGPTAHPV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m665-l GNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRAVRRIENIRLLRQHQFPEDAGPTAHPV 310 320 330 340 350 360 370 380 390 400 410 420 a665-1. pep RPARYEEMNNFYTMTVYEKGAEVVRMYHTLLGEEGFQKGMKLYFQRHDGQAVTCDDFRAA III I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m665-l RPAΞYEEMNNFYTMTVYEKGAEVVRMYHTLLGEEGFQKGMKLYFQRHDGQAVTCDDFRAA 370 380 390 400 410 420 430 440 450 460 470 480 a665-l.pep MVDANGINLDQFALWYSQAGTPVLDAQGRLKNNVFELTIKQTVPPTPDMADKQPMMIPVK Ml I I I I I I I I I I I I I I I I I I I I MMM I I I MM I Ml I I I I I I I I Ml I I I I I I I II m665-l MADANGINLDQFALWYSQAGTPVLEAEGRLKNNIFELTVKQTVPPTPDMTDKQPMMIPVK 430 440 450 460 470 480 490 500 510 520 530 540 a665-l.pep IGLLNCNGEAVAFDYQGKRATEAVLLLTEAEQTFQFESVTEAVVPSLLRGFSAPVHLNYP ill I I I I I I I I I I I I I I I I I I I I I I I I I I II : I : I I I I I I I I I I I I I I I I I I I I I I m665-l VGLLNRNGEAVAFDYQGKRATEAVLLLTEAEQTFLLEGVTEAVVPΞLLRGFSAPVHLNYP 490 500 510 520 530 540 550 560 570 580 590 600 a665-l.pep YSDDDLLLLLAHDSDAFTRWEAAQTLYRRAVAANLAALΞDGVELPKHEKLLAAVEKVISD I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I m665-l YSDDDLLLLLAHDSDAFTRWEAAQTLYRRAVAANLATLSDGVELPKHEKLLAAVEKVISD 550 560 570 580 590 600 610 620 630 640 650 660 a665-l.pep DLLDNAFKALLLGVPSEAELWDGAENIDPLRYHQAREALLDILAVRFLPKWHELNRQAAK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I m665-l DLLDNAFKALLLGVPΞEAELWDGAENIDPLRYHQAREALLDTLAVHFLPKWHELNRQAAK 610 620 630 640 650 660 670 680 690 700 710 720 a665-l.pep QENQSYEYSPEAAGWRTLRNVCRAFVLRADPAHIETVAEKYAEMAQNMTHEWGILSAVNG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I IM I I I I I I I I I I I I I I I I I m665-l QENQSYEYΞPEAAGWRTLRNVCRAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNG 670 680 690 700 710 720 730 740 750 760 770 780 a665-l.pep NEΞDTRNRLLAQFADKFSDDALVMDKYFALVGSSRRSDTLQQVQTALQHPKFSLENPNKA I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I :| I I I I I I I I I I I I I I I m665-l NESDTRNRLLAQFADKFSDDALVMDKYFALVGSSRRΞDTLQQVRTALQHPKFSLENPNKA 730 740 750 760 770 780 790 800 810 820 830 840 a665-l.pep RSLIGΞFSRNVPHFHAEDGSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m665-l RSLIGSFSRNVPHFHAEDGSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNL 790 800 810 820 830 840 850 860 a665-l.pep VKQALQRIRAQEGLSKDVGEIVGKILDX I I I I I I I I I I I I I I I I I I I I I I I I I I I I m665-l VKQALQRIRAQEGLSKDVGEIVGKILDX 850 860 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2171>: g666. seq 1 ATGCTTTGTA TGAATTATCA ATCAAACTCA GGCGAAGGAG TGCTTGTAGC 51 TAAAACATAT TTATTGACTG CATTGATAAT GTCTATGGTA ATCTCCGGAT 101 GTCAAGTCAT CCATGCCAAT CAAGGTAAGG TTAATACTAA TTCTGCTGTC 151 ATCGCAGGTG CAGACGCTCA CACGCCTGAA CATGTAACGG GACTGACCGA 201 ACAAAAGCAG GTGATTGCAA GTGATTTTAT AGTAGCGTCA GCCAATCCAT 251 TAGCAACACA AGCTGGCTAT GATATCTTAA AGCAAGGCGG TAGCGCTGCA 301 GATGCGATGG TGGCGGTGCA GACGACACTA AGCTTGGTAG AGCCACAGTC 351 GTCAGGCTTG GGCGGTGGTG CATTTGTGTT GTATTGGGAC AATACCGCCA 401 AAACATTGAC CACATTTGAT GGGCGTGAGA CGGCACCGAT GCGTGCGACG 451 CCAGAATTAT TTTTGGATAA AGATGGTTAA CCATTGAAAT TTATGGAAGC 501 GGTGGTCGCT CGGTAGGTAC GCCTGCTATC CCTAAACTGA This corresponds to the amino acid sequence <SEQ ID 2172; ORF 666.ng>: g666.pep 1 MLCMΝYQSΝS GEGVLVAKTY LLTALIMSMV ISGCQVIHAΝ QGKVΝTΝSAV 51 IAGADAHTPE HVTGLTEQKQ VIASDFIVAS AΝPLATQAGY DILKQGGSAA 101 DAMVAVQTTL SLVEPQSSGL GGGAFVLYWD ΝTAKTLTTFD GRETAPMRAT 151 PELFLDKDGX PLKFMEAWA RXVRLLSLΝ* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2173>: m666. seq 1 ATGCCTTGTA TGAATCATCA ATCAAACTCA GGCGAAGGAG TGCTTGTGGC 51 TAAAACATAT TTATTGACTG CATTGATAAT GTCTATGACA ATCTCTGGAT 101 GTCAAGTCAT CCATGCCAAT CAAGGTAAGG TTAATACTCA TTCTGCTGTC 151 ATCACAGGTG CAGACGCTCA CACGCCTGAA CATGCAACGG GACTGACCGA 201 ACAAAAGCAG GTGATTGCAA GTGATTTTAT GGTAGCGTCA GCCAATCCAT 251 TAGCAACACA AGCTGGCTAT GATATCTTAA AGCAAGGCGG TAGCGCTGCA 301 GATGCGATGG TGGCGGTGCA GACGACACTA AGCTTGGTAG AGCCACAGTC 351 GTCAGGCTTG GGCGGTGGTG CATTTGTGTT GTATTGGGAT AATACCGCCA 401 AAACATTGAC CACATTTGAT GGGCGTGAGA CGGCACCGAT GCGTGCGACG 451 CCGGAATTAT TTTTGGATAA AGATGGTCAA CCATTGAAAT TTATGGAAGC 501 GGTGGTCGTG GTCGCTCGGT GGGTACGCCT GCTATCCCTA AACTGA This corresponds to the amino acid sequence <SEQ ID 2174; ORF 666>: m666.pep 1 MPCMΝHQSΝS GEGVLVAKTY LLTALIMSMT ISGCQVIHAΝ QGKVΝTHSAV 51 ITGADAHTPE HATGLTEQKQ VIASDFMVAS AΝPLATQAGY DILKQGGSAA 101 DAMVAVQTTL SLVEPQSSGL GGGAFVLYWD ΝTAKTLTTFD GRETAPMRAT 151 PELFLDKDGQ PLKFMEAVW VARWVRLLSL Ν* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m666/g666 93.9% identity in 181 aa overlap 10 20 30 40 50 60 m666.pep MPCMΝHQSΝSGEGVLVAKTYLLTALIMSMTISGCQVIHAΝQGKVΝTHSAVITGADAHTPE I II I: II II II I II II II I II II II II I : I II I II II II I II II I : II I |: II II II I I g666 MLCMΝYQSΝSGEGVLVAKTYLLTALIMSMVISGCQVIHAΝQGKVΝTΝSAVIAGADAHTPE 10 20 30 40 50 60 70 80 90 100 110 120 m666.pep HATGLTEQKQVIASDFMVASAΝPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQSSGL I: II II II II II II I I : II II II I II II I I II II II II II II II II II II II II II II II g666 HVTGLTEQKQVIASDFIVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQSSGL 70 80 90 100 110 120 130 140 150 160 170 180 m666.pep GGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVWVARWVRLLSL II I II II II I II II I I II II I II II II II II II II II II II II II II I II llllll g666 GGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGXPLKFMEAW—ARXVRLLSL 130 140 150 160 170 m666.pep NX I I g666 NX 180 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2175>: a666. seq 1 ATGCCTTGTA TGAATCATCA ATCAAACTCA GGCGAAGGAG TGCTTGTGGC 51 TAAAACATAT TTATTGACTG CATTGATAAT GTCTATGACA ATCTCTGGAT 101 GTCAAGTCAT CCATGCCAAT CAAGGTAAGG TTAATACTCA TTCTGCTGTC 151 ATCACAGGTG CAGACGCTCA CACGCCTGAA CATGCAACGG GACTGACCGA 201 ACAAAAGCAG GTGATTGCAA GTGATTTTAT GGTAGCGTCA GCCAATCCAT 251 TAGCAACACA AGCTGGCTAT GATATCTTAA AGCAAGGCGG TAGCGCTGCA 301 GATGCGATGG TGGCGGTGCA GACGACACTA AGCTTGGTAG AGCCACAGTC 351 GTCAGGCTTG GGCGGTGGTG CATTTGTGTT GTATTGGGAT AATACCGCCA 401 AAACATTGAC CACATTTGAT GGGCGTGAGA CGGCACCGAT GCGTGCGACG 451 CCGGAATTAT TTTTGGATAA AGATGGTCAA CCATTGAAAT TTATGGAAGC 501 GGTGGTCGTG GTCGCTCGGT GGGTACGCCT GCTATCCCTA AACTGA This corresponds to the amino acid sequence <SEQ ID 2176; ORF 666. a>: a666.pep 1 MPCMΝHQSΝS GEGVLVAKTY LLTALIMSMT ISGCQVIHAΝ QGKVΝTHSAV 51 ITGADAHTPE HATGLTEQKQ VIASDFMVAS AΝPLATQAGY DILKQGGSAA 101 DAMVAVQTTL SLVEPQSSGL GGGAFVLYWD ΝTAKTLTTFD GRETAPMRAT 151 PELFLDKDGQ PLKFMEAVW VARWVRLLSL Ν* m666/a666 100.0% identity in 181 aa overlap 10 20 30 40 50 60 m666 . pep MPCMΝHQSΝSGEGVLVAKTYLLTALIMSMTISGCQVIHAΝQGKVΝTHSAVITGADAHTPE M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M a666 MPCMΝHQSΝSGEGVLVAKTYLLTALIMSMTISGCQVIHAΝQGKVΝTHSAVITGADAHTPE 10 20 30 40 50 60 70 80 90 100 110 120 m666.pep HATGLTEQKQVIASDFMVASAΝPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQSSGL I II II I II II II II II II I II II I II II II I I II I II II II I II II I II II II II I II II a666 HATGLTEQKQVIASDFMVASAΝPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQSSGL 70 80 90 100 110 120 130 140 150 160 170 180 m666. pep GGGAFVLYWDΝTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAWWARWVRLLSL MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a666 GGGAFVLYWDΝTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAWWARWVRLLSL 130 140 150 160 170 180 m666.pep NX I I a666 NX The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2177>: g667. seq 1 atgcggtttg tcttctgttt gggcgGAGAG ATAGtttctg atccgtgtga 51 tttccAtttg gtattcgtcc gcgtcgaatc tgccgctgAc CAGAcagaaa 101 cgCAGataca tCaaatacgt attcacggca tcggtttcgc aatAAttgcg 151 GAtttccttc agcgtgcccg cgtgGAacgc ttcccacact ttgctgccgt 201 ccataCCCAg ctTGCCCGGA AAGCCGCACA GTTTcgcCat atcgtccagC 251 GGCACATTcg ccctcggctG GTAAAGCGCG AGCAAATCCA TCAAATCGCA 301 GTGGCGTTGG TGATAACGGC TGATGTAGTT GTTCCActtg AAATCGCGGC 351 tgtcgccgAA ATCGccgTCG CCCGTATCCC AATAGCGCGC GGCGTTGATG 401 CCGTATATCA GGGAGCGGTA ATGCAGTACG GGCAGGTCGA AACCGCCGCC 451 GTTCCAGCTG ACCAGTTGCG GCGTATGTTT TTCAACCAAT TCGAAAAACT 501 TGGCAATCAC GACTTCTTCG CCATCGTCCA TCTCGCCGAT GGTGCCGACA 551 TGAACCTTGT CCTGCCCCCA GCGCATACAG CAGGAAACCG CCACAACCTG 601 ATGGAGGTGG TGCTGCATAA AATCGCCGCC GGTCTGTGCG CGGCGTTTCT 651 GCTGCGCGAA CAGCACCACT TCGTCATCCG GCAGGGAAGA CGGCAAGTCA 701 TACAACGTAC GGATACCCTG CACATCGGGT ACGGTTTCAA TATCGAAAGC 751 CAAAATCGTA TTCATGGCAg tACCTTGCAT tcaAAAACAG ACtTGCGCCT 801 ATTgTgtcaT TAA This corresponds to the amino acid sequence <SEQ ID 2178; ORF 667.ng>: g667.pep 1 MRFVFCLGGE IVSDPCDFHL VFVRVESAAD QTETQIHQIR IHGIGFAIIA 51 DFLQRARVER FPHFAAVHTQ LARKAAQFRH IVQRHIRPRL VKREQIHQIA 101 VALVITADW VPLEIAAVAE IAVARIPIAR GVDAVYQGAV MQYGQVETAA 151 VPADQLRRMF FΝQFEKLGΝH DFFAIVHLAD GADMΝLVLPP AHTAGΝRHΝL 201 MEWLHKIAA GLCAAFLLRE QHHFVIRQGR RQVIQRTDTL HIGYGFΝIES 251 QΝRIHGSTLH SKTDLRLLCH * The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2179>: m667.seq (PARTIAL) 1 ATGCGGCTTT TCCCCGGCTT GTGCGGACAG GTAATTCCGC ATCCGTTTGA 51 TTTCCATTTC GTATTCGTCC GCATCCAGCC TGCCGCTGAC CAGACAGAAA 101 CGCAGGTACA TCAGATAAGT GTTTGCCGCG TCGGTTTCGC AATAATTGCG 151 GATTTCCTTC AGCCTGCCCG TATGGAATGC CTCCCAAACC TTGCTGCCGT 201 CCATACCCAG CTTGCCCGGA AAACCGCACA GTTTCGCCAT ATCGTCCAGC 251 GGCACGTTTG CCCTCGGCTG GTAAAGCGCG AGCAAATCCA TCAAATCGCA 301 GTGGCGTTGG TGATAACGGC TGATGTAGTT GTTCCACTTG AAATCGCGGC 351 TGTCGCCGAA ATCGCCGTCG CCCATATCCC AATAGCGCGC GGCGTTGATG 401 CCGTATATCA GGGAGCGGTA ATGCAGTACG GGCAGATCGA AACCGCCGCC 451 GTTCCAACTG ACCAGTTGCG GCGTATGTTT TTCAATCAAT TCGAAAAATT 501 TAGCAATGAC CACTTCCTCG CCGTCATCCA TCTCGCCGAT GGTGCCGACA 551 TGTACTTTAT CCTGCCCCCA ACGCATGCAG CACGAAATCG CCACAACCTG 601 ATGAAGATGA TGCTGCATAA AATCGCCGCC CGTCTGAGCA CGGCGTTTGT 651 GCTGGGCAAT CAGCACCACT TG... This corresponds to the amino acid sequence <SEQ ID 2180; ORF 667>: m667.pep (partial) 1 MRLFPGLCGQ VIPHPFDFHF VFVRIQPAAD QTETQVHQIS VCRVGFAIIA 51 DFLQPARMEC LPΝLAAVHTQ LARKTAQFRH IVQRHVCPRL VKREQIHQIA 101 VALVITADW VPLEIAAVAE IAVAHIPIAR GVDAVYQGAV MQYGQIETAA 151 VPTDQLRRMF FΝQFEKFSΝD HFLAVIHLAD GADMYFILPP THAARΝRHΝL 201 MKMMLHKIAA RLSTAFVLGΝ QHHL... Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m667/g667 75.0% identity in 224 aa overlap 10 20 30 40 50 60 m667.pep MRLFPGLCG Q VIPHPFDFHFVFVRIQPAADQTETQVHQISVCRVGFAIIADFLQPARMEC g667 MRFVFCLGGEIVSDPCDFHLVFVRVESAADQTETQIHQIRIHGIGFAIIADFLQRARVER 10 20 30 40 50 60 70 80 90 100 110 120 m667. pep LPNLAAVHTQLARKTAQFRHIVQRHVCPRLVKREQIHQIAVALVITADVWPLEIAAVAE : MM II II II II M II II I II I I M II I II II I II II I II I II II II I II II II II I I g667 FPHFAAVHTQLARKAAQFRHIVQRHIRPRLVKREQIHQIAVALVITADVWPLEIAAVAE 70 80 90 100 110 120 130 140 150 160 170 180 m667. pep IAVAHIPIARGVDAVYQGAVMQYGQIETAAVPTDQLRRMFFNQFEKFSNDHFLAVIHLAD I II M I II II II II II II I II I II I: II II I I : I II II I I II II I MM I: M : II II g667 IAVARIPIARGVDAVYQGAVMQYGQVETAAVPADQLRRMFFNQFEKLGNHDFFAIVHLAD 130 140 150 160 170 180 190 200 210 220 m667.pep GADMYFILPPTHAARNRHNLMKMMLHKIAARLSTAFVLGNQHHL I I II : : II I : I : I I II II I ::: I I II II I : II M MM: g667 GADMNLVLPPAHTAGNRHNLMEWLHKIAAGLCAAFLLREQHHFVIRQGRRQVIQRTDTL 190 200 210 220 230 240 g 667 HIGYGFNIESQNRIHGSTLHSKTDLRLLCHX 250 260 270 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2181>: a667. seq 1 ATGCGGTTTG TCTTCTGTTT GGGCGGAGAG ATAGTTTCTG ATCCGCTTGA 51 TTTCCATTTC GTATTCGTCT GCGTCGAATC TGCCGCTGAC CAGACAGAAA 101 CGCAGATACA TCAGATAGGT ATTTACCGCA TCGGTTTCGC AATAATTGCG 151 GATTTCCTTC AGCCTGCCCG CGTGGAACGC CTCCCACACC TTGCTGCCGT 201 CCATACCCAG CTTGCCCGGA AAACCGCACA GTTTCGCCAT ATCGTCCAAC 251 GGCACATTCG CCCTCGGCTG GTAAAGCGCG AGCAAATCCA TCAAATCGCA 301 ATGACGTTGG TGGTAGCGGC TGATGTAGTT GTTCCACTTG AAATCGCGGC 351 TGTCGCCGAA ATCGCCGTCG CCCATATCCC AATAGCGCGC GGCGTTGATG 401 CCGTGTAGCA GCGAACGGTA ATGCAGAACC GGCAGGTCGA AACCGCCGCC 451 GTTCCAACTG ACCAGTTGCG GCGTATGTTT TTCAATCAAC TCGAAAAATT 501 TGGCGATAAC CACTTCCTCG CCGTCATCCA TCTCGCCGAT TGTACCGACA 551 TGGACTTTAT CCTGCCCCCA ACGCATGCAG CACGAAATCG CCACAATCTG 601 ATGAAGATGA TGCTGCATAA AATCCCCACC CGTCTGAGCA CGGCGTTTTT 651 GCTGGGCAAA CAGCACCACT TCATCGTCGG GCAGCGAGGA CGGCAAGTCA 701 TACAGCGTAC GGATACACTG CACATCGGGT ACGGTTTCAA TATCGAAAGC 751 CAAAATCGTG GTCATGACAG CACCTTGTAT TTAAAA.CAG ACTTGCGCCT 801 ATTGTGTCAT TAA This corresponds to the amino acid sequence <SEQ ID 2182; ORF 667.a>: a667.pep 1 MRFVFCLGGE IVSDPLDFHF VFVCVESAAD QTETQIHQIG IYRIGFAIIA 51 DFLQPARVER LPHLAAVHTQ LARKTAQFRH IVQRHIRPRL VKREQIHQIA 101 MTLWAADW VPLEIAAVAE IAVAHIPIAR GVDAV*QRTV MQΝRQVETAA 151 VPTDQLRRMF FΝQLEKFGDΝ HFLAVIHLAD CTDMDFILPP THAARΝRHΝL 201 MKMMLHKIPT RLSTAFLLGK QHHFIVGQRG RQVIQRTDTL HIGYGFΝIES 251 QΝRGHDSTLY LKXDLRLLCH * m667/a667 79.0% identity in 224 aa overlap 10 20 30 40 50 60 m667.pep MRLFPGLCGQVIPHPFDFHFVFVRIQPAADQTETQVHQISVCRVGFAIIADFLQPARMEC II: I I :: : I : II II II I : : II II II II : I I I : : I M M II I II II I I M I a667 MRFVFCLGGEIVSDPLDFHFVFVCVESAADQTETQIHQIGIYRIGFAIIADFLQPARVER 10 20 30 40 50 60 70 80 90 100 110 120 m667. pep LPΝLAAVHTQLARKTAQFRHIVQRHVCPRLVKREQIHQIAVALVITADVWPLEIAAVAE II : I II II II II II I I II II I II I I : I I II II II II II MM I :: I II II II II II I II a667 LPHLAAVHTQLARKTA Q FRHIVQRHIRPRLVKREQIHQIAMTLWAADVWPLEIAAVAE 70 80 90 100 110 120 130 140 150 160 170 180 m667. pep IAVAHIPIARGVDAVYQGAVMQYGQIETAAVPTDQLRRMFFNQFEKFSNDHFLAVIHLAD II II II I II II I II I I MM I : I I II I II II II I II II I : I II :: : I II II I II II a667 IAVAHIPIARGVDAVXQRTVMQNRQVETAAVPTDQLRRMFFNQLEKFGDNHFLAVIHLAD 130 140 150 160 170 180 190 200 210 220 m667. pep GADMYFILPPTHAARNRHNLMKMMLHKIAARLSTAFVLGNQHHL Ml II I II II I II II II II I II II I I MMMMIMMM a667 CTDMDFILPPTHAARNRHNLMKMMLHKIPTRLSTAFLLGKQHHFIVGQRGRQVIQRTDTL 190 200 210 220 230 240 a667 HIGYGFNIESQNRGHDSTLYLKXDLRLLCHX 250 260 270 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2183>: g669. seq 1 ATGCGCCGCA TCGTTAAAAA ACACCAGCCC GTAAACGCGC CACATATCGT 51 TTTGGAAATT CGGATAATGA AACTGCATCG CGCGTTTGTC TTCCTTGGGC 101 GGAAACGTCC CCATCATCAT GACCGCAGCC TTCGGCGGCA ACACGGGATC 151 GAAGGGATGG GTTTCGATTT CAAGCAGATT TTCAGACACG TTCAATCCTC 201 CAACAGGCAA AGCGGCAGAC AGCCGGTTTG CACCAAACCG CCAAACACGG 251 CAAGCCTTCA AACAGCATTA TCACGCCCTG CCGTTTTCGG TTACAATGCC 301 GACATCAAAC GGATACTGTA A This corresponds to the amino acid sequence <SEQ ID 2184; ORF 669.ng>: g669.pep 1 MRRIVKKHQP VΝAPHIVLEI RIMKLHRAFV FLGRKRPHHH DRSLRRQHGI 51 EGMGFDFKQI FRHVQSSΝRQ SGRQPVCTKP PΝTASLQTAL SRPAVFGYΝA 101 DIKRIL* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2185>: m669.seq 1 ATGCGCCGCA TCATTAAAAA ACACCAGCCC ATAAACGCGC CACATATCGT 51 TTTGGAAATT CGGATAATGA AACTGCATCG CGCGTTTGTC TTCCTTGGGC 101 GGAAACGTCC CCATCATCAT GACAGCAGCC TTCGGCGGCA ACACGGGATC 151 GAAGGGATGG GTTTCGATTT CAAGCAGATT TTCAGACACG TTCAATCCTC 201 CAACAGGCAA AACGGCAGAC AGCCGGTTTG CACCAAACCG CCAAACACGG 251 CAAGCCTTCA AACAGCATTA TCACGCCCTG CCGTTTTCGG TTACAATGCC 301 GACATCAAAC GGATACTGTA A This corresponds to the amino acid sequence <SEQ ID 2186; ORF 669>: m669.pep 1 MRRIIKKHQP IΝAPHIVLEI RIMKLHRAFV FLGRKRPHHH DSSLRRQHGI 51 EGMGFDFKQI FRHVQSSΝRQ ΝGRQPVCTKP PΝTASLQTAL SRPAVFGYΝA 101 DIKRIL* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m669/g669 96.2% identity in 106 aa overlap 10 20 30 40 50 60 m669.pep MRRIIKKHQPIΝAPHIVLEIRIMKLHRAFVFLGRKRPHHHDSSLRRQHGIEGMGFDFKQI I I I I : I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M M M M M M M M M g669 MRRIVKKHQPVΝAPHIVLEIRIMKLHRAFVFLGRKRPHHHDRSLRRQHGIEGMGFDFKQI 10 20 30 40 50 60 70 80 90 100 m669.pep FRHVQSSNRQNGRQPVCTKPPNTASLQTALSRPAVFGYNADIKRILX II I II I I II I : I II II I I II II II II II II II II II II I II II II I I g669 FRHVQSSNRQSGRQPVCTKPPNTASLQTALSRPAVFGYNADIKRILX 70 80 90 100 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2187>: a669.seq 1 ATGCGCCGCA TCATTAAAAA ACACCAGCCC GTAAACGCGC CACATATCGT 51 TTTGGAAATT CGGATAATGA AACTGCATCG CGCGTTTGTC TTCCTTGGGC 101 GGAAACGTCC CCATCATCAT GACCGCAGCC TTCGGCGGCA ACACGGAATC 151 GAAGGGATGG GTTTCGATTT CAAGCAGATT TTCAGACACG TTCAATCCTC 201 CAACAGGCAA AACGGCAGAC AGCCGGTTTG CACCAAACCG CCAAACACGG 251 CAAGCCTTCA AACAGCATTA TCACGCCCTG CCGTTTTCGG TTACAATGCC 301 GACATCAAAC GGATACTGTA A This corresponds to the amino acid sequence <SEQ ID 2188; ORF 669. a>: a669.pep 1 MRRIIKKHQP VΝAPHIVLEI RIMKLHRAFV FLGRKRPHHH DRSLRRQHGI 51 EGMGFDFKQI FRHVQSSΝRQ ΝGRQPVCTKP PΝTASLQTAL SRPAVFGYΝA 101 DIKRIL* m669/a669 98.1% identity in 106 aa overlap 10 20 30 40 50 60 m669.pep MRRIIKKHQPIΝAPHIVLEIRIMKLHRAFVFLGRKRPHHHDSSLRRQHGIEGMGFDFKQI II I I II II I I : II I II II II II II II II II I II I I II II II MMMMMMMMM a669 MRRIIKKHQPVΝAPHIVLEIRIMKLHRAFVFLGRKRPHHHDRSLRRQHGIEGMGFDFKQI 10 20 30 40 50 60 70 80 90 100 m669.pep FRHVQSSΝRQΝGRQPVCTKPPΝTASLQTALSRPAVFGYΝADIKRILX II I II II II II II I II I II II II I II II II II II II II I II I I II II a669 FRHVQSSΝRQΝGRQPVCTKPPΝTASLQTALSRPAVFGYΝADIKRILX 70 80 90 100 The following partial DΝA sequence was identified in N. gonorrhoeae <SEQ ID 2189>: g670.seq 1 ATGACTTGTT GCAGGAACTG CTTGGCGCGT TCGTGTTTCG GGTTGGTGAA 51 AAACGCTTCC GGCGTTTCGT CTTCAAGGAT TTGCCCTTTA TCGACGAAAA 101 TCACGCGGTC GGCAACTTCG CGGGCAAACC CCATTTCGTG GGTTACGCAC 151 ATCATCGTCA TGCCGCTTTC CGCCAAGTCT TTCATCACTT TCAACACTTC 201 GCCGACCATT TCGGGGTCGA GTGCGGAAGT CGGCTCGTCA AACAGCATCA 251 CGCGCGGCTC CATCGCCAGC CCGCGCGCAA TCGCCACGCG TTGCTGCTGG 301 CCGCCGGAAA GTTGGGAAGG GAAGGCGTCT TTTTTGTGTG CCAGTCCGAC 351 GCGTTCCAAA AGCTCCATTG CCTTTTTCTC CGCCTGTTCC GCATTTTGCC 401 CCTTAACCTT CATCGGTGCG AGGGTGATGT TGTCCAACAC GGTCAGGTGC 451 GGGTAG This corresponds to the amino acid sequence <SEQ ID 2190; ORF 670.ng>: g670.pep 1 MTCCRΝCLAR SCFGLVKΝAS GVSSSRICPL STKITRSATS RAΝPISWVTH 51 IIVMPLSAKS FITFΝTSPTI SGSSAEVGSS ΝSITRGSIAS PRAIATRCCW 101 PPESWEGKAS FLCASPTRSK SSIAFFSACS AFCPLTFIGA RVMLSΝTVRC 151 G* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2191>: m670.seq 1 ATGACCTGTT GCAGGAACTG CTTGGCGCGT TCGTGTTTCG GGTTGGTAAA 51 AAACGCTTCG GGCGTTTCGT CTTCGAGGAT TTGCCCTTTA TCGACGAAAA 101 TCACGCGGTC GGCAACTTCG CGGGCAAACC CCATTTCGTG GGTTACGCAC 151 ATCATCGTCA TGCCGCTTTC TGCCAAGTCT TTCATCACTT TCAACACTTC 201 GCCGACCATT TCGGGGTCGA GTGCGGAGGT CGGTTCGTCA AACAACATTA 251 CGCGCGGTTC CATCGCCAAA CCGCGTGCAA TCGCCACGCG TTGCTGCTGG 301 CCGCCGGAAA GTTGGGAAGG GAAGGCGTCT TTTTTGTGTG CCAGTCCGAC 351 GCGTTCCAAA AGCTCCATTG CCTTTTTCTC CGCCTGTTCC GCATTTTGCC 401 CCTTAACCTT CATCGGTGCG AGGGTAATGT TTTCCAACAC GGTCAGGTGC 451 GGGTAG This corresponds to the amino acid sequence <SEQ ID 2192; ORF 670>: m670.pep 1 MTCCRNCLAR SCFGLVKNAS GVSSSRICPL STKITRSATS RANPISWVTH 51 IIVMPLSAKS FITFNTSPTI SGSSAEVGSS NNITRGSIAK PRAIATRCCW 101 PPESWEGKAS FLCASPTRSK SSIAFFSACS AFCPLTFIGA RVMFSNTVRC 151 G* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m670/g670 98.0% identity in 151 aa overlap 10 20 30 40 50 60 m670.pep MTCCRΝCLARSCFGLVKΝASGVSSSRICPLSTKITRSATSRAΝPISWVTHIIVMPLSAKS II I II II II I II I II I II II I I II II II II II II II I I II II I I I II II II II I II I I II g670 MTCCRΝCLARSCFGLVKΝASGVSSSRICPLSTKITRSATSRAΝPISWVTHIIVMPLSAKS 10 20 30 40 50 60 70 80 90 100 110 120 m670.pep FITFΝTSPTISGSSAEVGSSΝΝITRGSIAKPRAIATRCCWPPESWEGKASFLCASPTRSK II II II I II II II II I II II I: II I II II : II II II II I II I II II II I II II II II I I I g670 FITFΝTSPTISGSSAEVGSSΝSITRGSIASPRAIATRCCWPPESWEGKASFLCASPTRSK 70 80 90 100 110 120 130 140 150 m670. pep SSIAFFSACΞAFCPLTFIGARVMFSΝTVRCGX II II II II II II I II II II I II I : I II II I II g670 SΞIAFFSACSAFCPLTFIGARVMLSΝTVRCGX 130 140 150 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2193>: a670. seq 1 ATGACCTGTT GCAGGAACTG CTTGGCGCGT TCGTGTTTCG GGTTGGTAAA 51 AAACGCTTCC GGCGTTTCGT CTTCGAGGAT TTGCCCTTTA TCGACGAAAA 101 TCACGCGGTC GGCAACTTCG CGGGCAAACC CCATTTCGTG GGTTACGCAC 151 ATCATGGTCA TACCGCTTTC CGCCAAGTCT TTCATCACTT TCAACACTTC 201 GCCGACCATT TCGGGGTCGA GTGCGGAGGT CGGTTCGTCA AACAACATTA 251 CGCGCGGTTC CATCGCCAAA CCGCGTGCAA TCGCCACGCG TTGCTGCTGG 301 CCGCCGGAAA GTTGGGAAGG GAAGGCGTCT TTTTTGTGTG CCAGTCCGAC 351 GCGTTCCAAA AGTTCCATCG CTTTTTTCTC TGCCTGTTCC GCATTTTGAC 401 CTTTAACCTT CATCGGTGCG AGGGTAATGT TTTCCAACAC GGTCAGGTGC 451 GGGTAG This corresponds to the amino acid sequence <SEQ ID 2194; ORF 670.a>: a670.pep 1 MTCCRΝCLAR SCFGLVKΝAS GVSSSRICPL STKITRSATS RAΝPISWVTH 51 IMVIPLSAKS FITFΝTSPTI SGSSAEVGSS ΝΝITRGSIAK PRAIATRCCW 101 PPESWEGKAS FLCASPTRSK SSIAFFSACS AF*PLTFIGA RVMFSNTVRC 151 G* m670/a670 98.0% identity in 151 aa overlap 10 20 30 40 50 60 m670.pep MTCCRNCLARSCFGLVKNASGVSSSRICPLSTKITRSATSRANPISWVTHIIVMPLSAKS I II II II I I II II I II II II II II II II II II II II II I II II II II II I I : I: II II II a670 MTCCRNCLARSCFGLVKNASGVSSSRICPLSTKITRSATSRANPISWVTHIMVIPLSAKS 10 20 30 40 50 60 70 80 90 100 110 120 m670.pep FITFNTSPTISGSSAEVGSSNNITRGSIAKPRAIATRCCWPPESWEGKASFLCASPTRSK MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a670 FITFNTSPTISGSSAEVGSSNNITRGSIAKPRAIATRCCWPPESWEGKASFLCASPTRSK 70 80 90 100 110 120 130 140 150 m670.pep SSIAFFSACSAFCPLTFIGARVMFSNTVRCGX II II II II II II I II II I II II II II II II I a670 SSIAFFSACSAFXPLTFIGARVMFSNTVRCGX 130 140 150 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2195>: g671.seq 1 ATGATCAGCA GGGTAACAAT CAAAACGCCT TTCAATGCAC CGAATACACC 51 GCCCAAAATG CGGTTGGCAA AGCCCAGACC GACCGCCGAA ACTGCGCCGG 101 TCAGCAGCGA ACGGAGCATT TTCTGGATCA GACAGGCAAT GACGAACAGG 151 GAAATGAATG ACAGagccaa TGCAAACAgg cggggTTGGA ACGaggCAAA 201 GGCGAGGTcg gcgaaggGTG CGGCaaAGAG TTTggcaaAA AAGAaggAAA 251 ccaccCATGC cACCATCgaa ccTGCTTCCG CAATCACGCC GCGCATCGTG 301 GAAATGACGA TGCAGGCGGC GATGACGGcg gAGGCGAGGA GGTCGGCAAT 351 GGGGAGGCTA TTCATTCGTT ACCTGGCCGG CGATGCCGTG CACGCGCAGT 401 TTGTTCAAAT CGCGTTCGGC ATCCCTTGCG TTTTTATAGT TGCTTGA This corresponds to the amino acid sequence <SEQ ID 2196; ORF 671.ng>: g671.pep 1 MISRVTIKTP FΝAPΝTPPKM RLAKPRPTAE TAPVSSERSI FWIRQAMTΝR 51 EMΝDRAΝAΝR RGWΝEAKARS AKGAAKSLAK KKETTHATIE PASAITPRIV 101 EMTMQAAMTA EARRSAMGRL FIRYLAGDAV HAQFVQIAFG IPCVFIVA* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2197>: m671. seq 1 ATGACCAGCA GGGTAACAAT CAAAACGCCT TTCAATGCAC CGAATACGCC 51 GCCCAAAATG CGGTTGGCAA AGCCCAAACC GACCGCCGAA ACTGCGCTGG 101 TCAGCAGCGA ACGGAGCATT TTCTGGATCA GACAGGCAAT GACGAACAGG 151 GAAATGAACG ACAGAGCCAA TGCAAACAGG CGGGGTTGGA ACGAGGCAAA 201 GGCGAGGTCG GCGAAGGAGG CGGCAAAGAG TTTGGCGAAA AAGAAGGAAA 251 CCACCCATGC CGCCATTGAG CCTGCCTCCG CAATCACGCC GCGCATCGCG 301 GATAGCACGA TGCAGGCGGC GATGACGGCG GAGACGAGGA GGTCGGCAAT 351 GGGGAGGCTA TTCATTCGTT ACCTGACCGG CGATACCGTG TACGCGCAAT 401 TTGTTCAAAT CGCGTTCGGC ATCCCTTGCG TTTTTATAGT TGCTTGA This corresponds to the amino acid sequence <SEQ ID 2198; ORF 671>: m671.pep 1 MTSRVTIKTP FΝAPΝTPPKM RLAKPKPTAE TALVSSERSI FWIRQAMTΝR 51 EMΝDRAΝAΝR RGWΝEAKARS AKEAAKSLAK KKETTHAAIE PASAITPRIA 101 DSTMQAAMTA ETRRSAMGRL FIRYLTGDTV YAQFVQIAFG IPCVFIVA* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m671/g671 91.9% identity in 148 aa overlap 10 20 30 40 50 60 m671.pep MTSRVTIKTPFΝAPΝTPPKMRLAKPKPTAETALVSSERSIFWIRQAMTΝREMΝDRAΝAΝR I II II I II I II II I II II II II II : II I II I II II II II I I II II II II II I I II I I! g671 MISRVTIKTPFΝAPΝTPPKMRLAKPRPTAETAPVSSERSIFWIRQAMTΝREMΝDRAΝAΝR 10 20 30 40 50 60 70 80 90 100 110 120 m671.pep RGWΝEAKARSAKEAAKSLAKKKETTHAAIEPASAITPRIADSTMQAAMTAETRRSAMGRL I I II II I II II I II II II II II II II : II II II II II I : : MMMM MMMM g671 RGWNEAKARSAKGAAKSLAKKKETTHATIEPASAITPRIVEMTMQAAMTAEARRSAMGRL 70 80 90 100 110 120 130 140 149 m671.pep FIRYLTGDTVYAQFVQIAFGIPCVFIVAX I I II I : I I : M II II I II II II II I II I I g671 FIRYLAGDAVHAQFVQIAFGIPCVFIVAX 130 140 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2199>: a671. seq 1 ATGACCAGCA GGGTAATAAT CAAAATGCCT TTCAATGCAC CGAATACGCC 51 GCCCAAAATG CGGTTGGCAA AGCCCAAACC GACCGCCGAA ACTGCCCCGG 101 TCAGCAGCGA GCGGAGTATT TTCTGGATCA GACAGGCAAT GACGAATAGG 151 GAAATGAACG ACAGAGCCAA TGCAAACAGG CGGGGTTGGA ACGATGCAAA 201 GGCGATGTCG GCGAAGGGTG CGGCAAAGAG TTTGGCGAAA AAAAAGGCAA 251 CCACCCATGC CGCCATTGAG CCAGCCTCCG CAATCACGCC GCGCATCGCG 301 GATAGCACGA TGCAGGCGGC GATGATGGCG GAGACGAGGA GGTCGGCAAC 351 GGGGAGGTTA TTCATTCGTT ACCTGACCGG CGATACCGTG TACGCGCAAT 401 TTGTTCAAAT CGCGTTCGGC ATCCCTTGCG TTTTTATAGT TGCTTGA This corresponds to the amino acid sequence <SEQ ID 2200; ORF 671.a>: a671.pep 1 MTSRVIIKMP FΝAPΝTPPKM RLAKPKPTAE TAPVSSERSI FWIRQAMTΝR 51 EMΝDRAΝAΝR RGWΝDAKAMS AKGAAKSLAK KKATTHAAIE PASAITPRIA 101 DSTMQAAMMA ETRRSATGRL FIRYLTGDTV YAQFVQIAFG IPCVFIVA* m671/a671 93.9% identity in 148 aa overlap 10 20 30 40 50 60 m671.pep MTSRVTIKTPFΝAPΝTPPKMRLAKPKPTAETALVSSERSIFWIRQAMTΝREMΝDRAΝAΝR Mill II I II II II II I I II II I I I I II II II II II II I II II II II II II I II II I a671 MTSRVIIKMPFΝAPΝTPPKMRLAKPKPTAETAPVSSERSIFWIRQAMTΝREMΝDRAΝAΝR 10 20 30 40 50 60 70 80 90 100 110 120 m671.pep RGWΝEAKARSAKEAAKSLAKKKETTHAAIEPASAITPRIADSTMQAAMTAETRRSAMGRL II MM II III II II I II II II II II II II II I II I II II I II II I I I I II I III a671 RGWΝDAKAMSAKGAAKSLAKKKATTHAAIEPASAITPRIADSTMQAAMMAETRRSATGRL 70 80 90 100 110 120 130 140 149 m671.pep FIRYLTGDTVYAQFVQIAFGIPCVFIVAX II I I II II II II II II II II II I II II II a671 FIRYLTGDTVYAQFVQIAFGIPCVFIVAX 130 140 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 2201>: g672. seq 1 ATGAGGAAAA TCCGCACCAA AATCTGCGGC ATCACCACAC CGGAAGACGC 51 ACTGTATGCC GCCCACGCCG GCGCAGACGC ATTGGGACTG GTTTTTTACC 101 CCCAAAGCCC CCGCGCTATC GACATCATTA AAGCACAAAA AATCGCCGCC 151 GCACTGCCGC CGTTTGTCAG CGTTGTCGCC CTTTTCGTCA ACGAAAGCGC 201 GCAAAACATC CGCCGCATCC TTGCCGAAGT GCCGATACAC ATCATCCAAT 251 TCCACGGCGA CGAAGACGAT GCATTCTGCC GGCAGTTCGA CCGCCCCTAT 301 ATTAAAGCCA TTCGTGTTCA GACGGCATCA GACATCCGAA ACGCCGCCAC 351 GCGCTTCCCC AACGCTCAGG CACTGCTGTT CGATGCCTAT CACCCTTCGG 401 AATACGGCGG CACCGGACAC CGCTTCGact GGacgctgtt ggcggAATAT 451 TCGGGCAAGC CGTGGGTGCT TGCCGGCGGG CTGACCCCTG AAAACGTCGG 501 CGAAGCCGTC CGCATCACCG GAGCGGAAGC GGTCGACGTA TCCGGCGGCG 551 TGGAAGCGTC TAAAGGCAAA AAAGACCCCG CCAAAGTCGC CGCCTTTATC 601 GCAACCGCCA ACCGCCTATC CCGTTAA This corresponds to the amino acid sequence <SEQ ID 2202; ORF 672.ng>: g672.pep 1 MRKIRTKICG ITTPEDALYA AHAGADALGL VFYPQSPRAI DIIKAQKIAA 51 ALPPFVSWA LFVNESAQNI RRILAEVPIH IIQFHGDEDD AFCRQFDRPY 101 IKAIRVQTAS DIRNAATRFP NAQALLFDAY HPSEYGGTGH RFDWTLLAEY 151 SGKPWVLAGG LTPENVGEAV RITGAEAVDV SGGVEASKGK KDPAKVAAFI 201 ATANRLSR* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2203>: m672.seq 1 ATGAGGAAAA TCCGCACCAA AATCTGCGGC ATCACCACAC CGGAAGACGC 51 AGCTGCCGCC GCAGCGGCAG GTGCGGATGC CGTCGGGCTG GTCTTTTTCC 101 AAGGCAGCAG CCGGGCCGTC GATATTGCCC GCGCCAAAAA AATCACCGCC 151 GCACTGCCGC CGTTTGTCAG CGTTGTCGCC CTTTTCGTCA ACGAAAGCGC 201 GCAAAACATC CGCCGCATCC TTGCCGAAGT GCCGATACAC ATCATCCAAT 251 TCCACGGCGA CGAAGACGAC GCATTCTGCC GCCAGTTCCA CCGCCCCTAT 301 ATCAAAGCCA TTCGTGTTCA GACGGCATCA GACATCCGAA ACGCCGCCAC 351 GCGCTTCCCC GACGCTCAGG CACTGCTGTT CGATGCCTAC CATCCTTCGG 401 AATACGGCGG CACCGGAAAC CGCTTCGACT GGACGCTGCT GGCGGAATAT 451 TCGGGCAAAC CGTGGGTGCT TGCCGGCGGG CTGACCCCTG AAAACGTCGG 501 CGAAGCCGTC CGCATCACCG GAGCGGAATC GGTCGATGTA TCCGGCGGTG 551 TGGAAGCGTC TAAAGGCAAA AAAGATGCCG CCAAAGTCGC CGCCTTTATC 601 GCAACCGCCA ACCGCCTATC CCGTTAA This corresponds to the amino acid sequence <SEQ ID 2204; ORF 672>: m672.pep 1 MRKIRTKICG ITTPEDAAAA AAAGADAVGL VFFQGSSRAV DIARAKKITA 51 ALPPFVSWA LFVΝESAQΝI RRILAEVPIH IIQFHGDEDD AFCRQFHRPY 101 IKAIRVQTAS DIRNAATRFP DAQALLFDAY HPSEYGGTGN RFDWTLLAEY 151 SGKPWVLAGG LTPENVGEAV RITGAESVDV SGGVEASKGK KDAAKVAAFI 201 ATANRLSR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m672/g672 91.3% identity in 208 aa overlap 10 20 30 40 50 60 m672.pep MRKIRTKICGITTPEDAAAAAAAGADAVGLVFFQGSSRAVDIARAKKITAALPPFVSWA II II I II II I II II II I II II I II : II I M I IMM M : I I : I I I I II I II I I g672 MRKIRTKICGITTPEDALYAAHAGADALGLVFYPQSPRAIDIIKAQKIAAALPPFVSWA 10 20 30 40 50 60 70 80 90 100 110 120 m672.pep LFVΝESAQΝIRRILAEVPIHIIQFHGDEDDAFCRQFHRPYIKAIRVQTASDIRΝAATRFP I II II II II II II II II II I II I II II II II II II I II II II II II II II II II II I II g672 LFVΝESAQΝIRRILAEVPIHIIQFHGDEDDAFCRQFDRPYIKAIRVQTASDIRΝAATRFP 70 80 90 100 110 120 130 140 150 160 170 180 m672. pep DAQALLFDAYHPSEYGGTGΝRFDWTLLAEYSGKPWVLAGGLTPEΝVGEAVRITGAESVDV MMMMMMMMMMMMMMMMMMMMMMMMMMMMM g672 ΝAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGGLTPEΝVGEAVRITGAEAVDV 130 140 150 160 170 180 190 200 209 m672.pep SGGVEASKGKKDAAKVAAFIATAΝRLSRX II II II II I II I II II I II II II I II I I g672 SGGVEASKGKKDPAKVAAFIATAΝRLSRX 190 200 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2205>: a672. seq 1 ATGAGGAAAA TCCGCACCAA AATCTGCGGC ATCACCACAC CGGAAGACGC 51 ACTGTATGCC GCCCACGCCG GCGCAGACGC ATTGGGACTG GTTTTTTACC 101 CCCAAAGCCC CCGCGCTGTC GACATCATTA AAGCACAAAA AATCACCGCC 151 GCACTGCCGC CGTTTGTCAG CGTTGTCGCC CTTTTCGTCA ACGAAAGCGC 201 GCAAAACATC CGCCGCATCC TTGCCGAAGT ACCGATACAC ATCATCCAAT 251 TCCACGGCGA CGAAGACGAC GCATTCTGCC GCCAGTTCCA CCGCCCCTAT 301 ATCAAGGCCA TTCGTGTTCA GACGGCATCA GACATCCGAA ACGCCGCCGA 351 CCGCTTCCCC GACGCTCAGG CACTGCTGTT CGATGCCTAC CATCCTTCGG 401 AATACGGCGG CACCGGACAC CGCTTCGACT GGACGCTGTT GGCGGAATAT 451 TCGGGCAAAC CGTGGGTGCT TGCCGGCGGG CTGACCCCTG AAAACGTCGA 501 CGAAGCCATC CGCATCACCG GAGCGGAAGC GGTCGATGTA TCCGGCGGCG 551 TGGAAGCGTC TAAAGGCAAA AAAGACCCAG CCAAAGTTGC CGCCTTTATC 601 GCAACCGCCA ACCGCCTATC CCGTTAA This corresponds to the amino acid sequence <SEQ ID 2206; ORF 672. a>: a672.pep 1 MRKIRTKICG ITTPEDALYA AHAGADALGL VFYPQSPRAV DIIKAQKITA 51 ALPPFVSWA LFVNESAQNI RRILAEVPIH IIQFHGDEDD AFCRQFHRPY 101 IKAIRVQTAS DIRNAADRFP DAQALLFDAY HPSEYGGTGH RFDWTLLAEY 151 SGKPWVLAGG LTPENVDEAI RITGAEAVDV SGGVEASKGK KDPAKVAAFI 201 ATANRLSR* m672/a672 91.8% identity in 208 aa overlap 10 20 30 40 50 60 m672. pep MRKIRTKICGITTPEDAAAAAAAGADAVGLVFFQGSSRAVDIARAKKITAALPPFVSWA II II II II II II II I II II II I I I : I I II : I I I II I : I : I I I I II I I II II I I a672 MRKIRTKICGITTPEDALYAAHAGADALGLVFYPQSPRAVDIIKAQKITAALPPFVSWA 10 20 30 40 50 60 70 80 90 100 110 120 m672. pep LFVNESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFHRPYIKAIRVQTASDIRNAATRFP II II II II I II II I II II II I II II II I I II II II II II I II II II II II II I I II III a672 LFVNESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFHRPYIKAIRVQTASDIRNAADRFP 70 80 90 100 110 120 130 140 150 160 170 180 m672. pep DAQALLFDAYHPSEYGGTGNRFDWTLLAEYSGKPWVLAGGLTPENVGEAVRITGAESVDV II II II II II II II II II I: II II II I I II II II II II II II I I II I I : II I I II : I II a672 DAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGGLTPENVDEAIRITGAEAVDV 130 140 150 160 170 180 190 200 209 m672.pep SGGVEASKGKKDAAKVAAFIATANRLSRX II II II II II II II II I II II I I II I II a672 SGGVEASKGKKDPAKVAAFIATANRLSRX 190 200 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2207>: g673. seq 1 ATGGATATTG AAACCTTCCT TGCAGGGGAA CGCGCCGCCG GCGGATACCG 51 TTGCGGCTTC GTGGCGATTG TCGGTCGTCC GAACGTGGGC AAATCAACGC 101 TGATGAACCA TCTCATCGGT CAGAAAATCA GTATTACCAG CAAAAAGGCG 151 CAGACGACGC GCAACCGCGT AACGGGGATT TATACCGACG ATACCGCGCA 201 GTTCGTGTTT GTCGATACGC CGGGCTTTCA AACCGACCAC CGCAACGCGC 251 TCAACGACAG GCTGAATCAA AATGTTACCG AGGCGCTCGG CGGTGTGGAT 301 GTGGTGGTTT TCGTCGTGGA GGCGATGCGC CTTACCGATG CCGACCGCGT 351 CGTGTTGAAA CAACTGCCCA AGCACACGCC GGTCATTTTA GTGATCAACA 401 AAATCGACAA GGACAAGGCG AAAGACCGTT ACGCGCTGGA GGCGTTTGTT 451 GCCCAAGTGC GCGCCGAATT TGAATTTGCG GCGGCGGAGG CGGTCAGTGC 501 GAAACACGGT TTGCGGATTG CCAACCTGTT GGAGCTGCTC AAGCCGTATC 551 TGCCCGAAAG CGTACCGATG TATCCCGAAG ACATGGTTAC GGACAAATCG 601 GCGCGTTTTT TGGCGATGGA AATCGTGCGT GAAAAACTCT TCCGCTATTT 651 GGGCGAGGAG CTGCCTTATG CGATGAACGT CGAAGTGGAG CAGTTTGAAG 701 AGGGAGACGG TTTGAACCGC ATCTACatcg cCGTTTTGGT CGACAAAGAA 751 AGCCAAAAGG CGATTTTGAT CGGTAAAGGC GGGGAGCGTT TGAAAAAAAT 801 TTCCACCGAA GCGCGGCTGG ATATGGAAAA ACTGTTTGAT AACAAAGTAT 851 TTTTGAAGGT CTGGGTCAAA GTCAAATCCG GTTGGGCAGA CGACATTCGC 901 TTCCTGCGCG AGCTGGGTTT GTAG This corresponds to the amino acid sequence <SEQ ID 2208; ORF 673.ng>: g673.ρep 1 MDIETFLAGE RAAGGYRCGF VAIVGRPNVG KSTLMNHLIG QKISITSKKA 51 QTTRNRVTGI YTDDTAQFVF VDTPGFQTDH RNALNDRLNQ NVTEALGGVD 101 VWFWEAMR LTDADRWLK QLPKHTPVIL VINKIDKDKA KDRYALEAFV 151 AQVRAEFEFA AAEAVSAKHG LRIANLLELL KPYLPESVPM YPEDMVTDKS 201 ARFLAMEIVR EKLFRYLGEE LPYAMNVEVE QFEEGDGLNR IYIAVLVDKE 251 SQKAILIGKG GERLKKISTE ARLDMEKLFD NKVFLKVWVK VKSGWADDIR 301 FLRELGL* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2209>: m673. seq 1 ATGGATATTG AAACCTTCCT TGCAGGGGAA CGCGCCGCCG GCGGATACCG 51 TTGCGGCTTC GTAGCGATTG TCGGCCGTCC GAACGTGGGC AAATCAACGC 101 TGATGAACCA TCTCATCGGT CAGAAAATCA GTATTACCAG CAAAAAGGCG 151 CAGACGACGC GCAACCGCGT AACGGGGATT TATACCGACG ATACCGCGCA 201 GTTCGTGTTT GTCGATACGC CCGGCTTTCA AACCGACCAC CGCAACGCGC 251 TCAACGACAG GCTGAATCAA AATGTTACCG AGGCGCTCGG CGGCGTGGAT 301 GTGGTGGTTT TCGTCGTGGA GGCGATGCGC TTTACCGATG CCGACCGCGT 351 CGTGTTGAAA CAACTGCCCA AGCACACGCC GGTCATTTTA GTGGTCAACA 401 AAATCGACAA GGACAAGGCG AAAGACCGTT ACGCGCTGGA GGCGTTTGTT 451 GCCCAAGTGC GCGCCGAATT TGAATTTGCG GCGGCGGAGG CGGTCAGCGC 501 GAAACACGGA TTGCGGATTG CCAACCTGTT GGAGCTGATT AAGCCGTATC 551 TGCCCGAAAG CGTGCCGATG TATCCCGAAG ATATGGTTAC GGACAAATCG 601 GCGCGTTTTT TGGCGATGGA AATCGTGCGT GAAAAATTGT TCCGCTATTT 651 GGGCGAGGAA TTGCCTTATG CGATGAACGT CGAAGTGGAG CAGTTTGAAG 701 AGGAAGACGG TTTGAACCGC ATCTATATCG CCGTTTTGGT CGATAAGGAA 751 AGCCAAAAGG CAATTTTAAT CGGTAAAGGC GGAGAACGTT TGAAGAAAAT 801 TTCCACCGAA GCGCGGTTGG ATATGGAAAA ACTGTTTGAT ACCAAAGTAT 851 TTTTGAAGGT CTGGGTCAAA GTCAAATCCG GTTGGGCGGA CGACATCCGC 901 TTCCTGCGCG AGCTGGGTTT GTAG This corresponds to the amino acid sequence <SEQ ID 2210; ORF 673>: m6 3.pep 1 MDIETFLAGE RAAGGYRCGF VAIVGRPNVG KSTLMNHLIG QKISITSKKA 51 QTTRNRVTGI YTDDTAQFVF VDTPGFQTDH RNALNDRLNQ NVTEALGGVD 101 VWFWEAMR FTDADRWLK QLPKHTPVIL WNKIDKDKA KDRYALEAFV 151 AQVRAEFEFA AAEAVSAKHG LRIANLLELI KPYLPESVPM YPEDMVTDKS 201 ARFLAMEIVR EKLFRYLGEE LPYAMNVEVE QFEEEDGLNR IYIAVLVDKE 251 SQKAILIGKG GERLKKISTE ARLDMEKLFD TKVFLKVWVK VKSGWADDIR 301 FLRELGL* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m673/g673 98.4% identity in 307 aa overlap 10 20 30 40 50 60 m673 . pep MDIETFLAGERAAGGYRCGFVAIVGRPΝVGKSTLMΝHLIGQKISITSKKAQTTRΝRVTGI M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M g673 MDIETFLAGERAAGGYRCGFVAIVGRPΝVGKSTLMΝHLIGQKISITSKKAQTTRΝRVTGI 10 20 30 40 50 60 70 80 90 100 110 120 m673. pep YTDDTAQFVFVDTPGFQTDHRΝALΝDRLΝQΝVTEALGGVDVWFWEAMRFTDADRWLK g673 YTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVWFWEAMRLTDADRWLK 70 80 90 100 110 120 130 140 150 160 170 180 m673. pep QLPKHTPVILWNKIDKDKAKDRYALEAFVAQVRAEFEFAAAEAVSAKHGLRIANLLELI II I II II II II M II II II II II II II I II I II I I I II II I II II II I II I I II II II I: g673 QLPKHTPVILVINKIDKDKAKDRYALEAFVAQVRAEFEFAAAEAVSAKHGLRIANLLELL 130 140 150 160 170 180 190 200 210 220 230 240 m673.pep KPYLPEΞVPMYPEDMVTDKSARFLAMEIVREKLFRYLGEELPYAMNVEVEQFEEEDGLNR II I II II II I II II II I II I II II II II II I II I II II II I II II I I II I I II I I II M g673 KPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLGEELPYAMNVEVEQFEEGDGLNR 190 200 210 220 230 240 250 260 270 280 290 300 m673.pep IYIAVLVDKESQKAILIGKGGERLKKISTEARLDMEKLFDTKVFLKVWVKVKSGWADDIR II II II II II II II II I II I II II II II II I I II II I II M II II II II II I II II II II g673 IYIAVLVDKESQKAILIGKGGERLKKISTEARLDMEKLFDNKVFLKVWVKVKSGWADDIR 250 260 270 280 290 300 m673.pep FLRELGLX llllllll g673 FLRELGLX The following partial DNA sequence was identified in N meningitidis <SEQ ID 2211>: a673.seq 1 ATGGATATTG AAACCTTCCT TGCAGGGGAA CGCGCCGCCG ACGGATACCG 51 TTGCGGCTTC GTAGCGATTG TCGGCCGTCC GAACGTGGGC AAATCAACGC 101 TGATGAATCA TCTCATCGGT CAGAAAATCA GTATTACCAG CAAAAAGGCG 151 CAGACGACGC GCAACCGCGT AACGGGGATT TATACCGACG ATACCGCGCA 201 GTTTGTGTTT GTCGATACGC CCGGTTTTCA AACCGACCAC CGCAACGCGC 251 TCAACGACCG TTTGAATCAA AACGTTACCG AGGCACTCGG CGGCGTGGAT 301 GTGGTGGTTT TCGTCGTGGA AGCGATGCGT TTTACCGATG CCGACCGCGT 351 CGTGTTGAAA CAACTGCCCA AGCACACGCC GGTCATTTTA GTGGTCAACA 401 AAATCGATAA GGACAAGGCG AAAGACCGTT ACGCGCTGGA GGCGTTTGTT 451 GCCCAGGTGC GCGCCGAATT TGAATTTGCG GCGGCGGAGG CGGTCAGCGC 501 GAAACACGGA TTGCGGATTG CCAACCTGTT GGAGCTGATT AAGCCGTATC 551 TGCCCGAAAG CGTGCCGATG TATCCCGAAG ATATGGTTAC GGACAAATCG 601 GCGCGTTTTT TAGCGATGGA AATCGTGCGT GAAAAATTGT TCCGCTATTT 651 GGGCGAGGAA TTGCCTTATG CGATGAACGT CGAAGTGGAG CAGTTTGAAG 701 AGGAAGACGG TTTGAACCGC ATCTATATCG CCGTTTTGGT CGATAAGGAA 751 AGCCAAAAGG CGATTTTAAT CGGCAAAGGC GGGGAGCGTT TGAAGAAAAT 801 TTCCACCGAA GCGCGGTTGG ATATGGAAAA ACTGTTTGAT ACCAAAGTAT 851 TTTTGAAGGT CTGGGTCAAA GTCAAATCCG GTTGGGCGGA CGACATCCGC 901 TTCCTGCGCG AGCTGGGTTT GTAG This corresponds to the amino acid sequence <SEQ ID 2212; ORF 673. a>: a673.pep 1 MDIETFLAGE RAADGYRCGF VAIVGRPNVG KSTLMNHLIG QKISITSKKA 51 QTTRNRVTGI YTDDTAQFVF VDTPGFQTDH RNALNDRLNQ NVTEALGGVD 101 VWFWEAMR FTDADRWLK QLPKHTPVIL WNKIDKDKA KDRYALEAFV 151 AQVRAEFEFA AAEAVSAKHG LRIANLLELI KPYLPESVPM YPEDMVTDKS 201 ARFLAMEIVR EKLFRYLGEE LPYAMNVEVE QFEEEDGLNR IYIAVLVDKE 251 SQKAILIGKG GERLKKISTE ARLDMEKLFD TKVFLKVWVK VKSGWADDIR 301 FLRELGL* 673/a673 99.7% identity in 307 aa overlap 10 20 30 40 50 60 m673.pep MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKISITSKKAQTTRNRVTGI I I II I I II I II I I II II II I I II II II II II II II I II II II II II I II I II II II I II a673 MDIETFLAGERAADGYRCGFVAIVGRPNVGKSTLMNHLIGQKISITSKKAQTTRNRVTGI 10 20 30 40 50 60 70 80 90 100 110 120 m673. pep YTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDWVFWEAMRFTDADRWLK I II II I II II II II I I II I II I II II I II II II II II II II II II II II II II II II II I a 673 YTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDWVFWEAMRFTDADRWLK 70 80 90 100 110 120 130 140 150 160 170 180 m673.pep QLPKHTPVILVVNKIDKDKAKDRYALEAFVAQVRAEFEFAAAEAVSAKHGLRIANLLELI M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M a673 QLPKHTPVILWNKIDKDKAKDRYALEAFVAQVRAEFEFAAAEAVSAKHGLRIANLLELI 130 140 150 160 170 180 190 200 210 220 230 240 m673.pep KPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLGEELPYAMNVEVEQFEEEDGLNR M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M a 673 KPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLGEELPYAMNVEVEQFEEEDGLNR 190 200 210 220 230 240 250 260 270 280 290 300 m673. pep IYIAVLVDKESQKAILIGKGGERLKKISTEARLDMEKLFDTKVFLKVWVKVKSGWADDIR M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M a673 IYIAVLVDKESQKAILIGKGGERLKKISTEARLDMEKLFDTKVFLKVWVKVKSGWADDIR 250 260 270 280 290 300 m673.pep FLRELGLX II I I II I I a673 FLRELGLX The following partial DNA sequence was identified inN gonorrhoeae <SEQ ID 2213>: g67 . seq 1 ATGAAAACAG CCCGCCGCCG TTCCCGCGAG CTTGCCGTAC AAGCCGTTTA 51 CCAATCCCTT ATCAACCGCA CCGCCGCGCC CGAAATTGCT AAAAACATCC 101 GCGAAATGTC CGACTTTGCC AAAGCGGACG AAGAATTGTT CAACAAACTC 151 TTCTTCGGCA CACAAACCAA TGCAGCGGAC TACATCCAAA AAATCCGCCC 201 GCTGCTCGAC AGGGACGAAA AAGACCTCAA CCCCATCGAA CGCGCCGTTT 251 TGCTGACCGC CTGCCACGAG CTTTCCGCTA TGCCCGAAAC GCCCTACCCC 301 GTCATTATCA ACGAAGCCAT CGAAGTTACC AAAACCTTCG GCGGCACGGA 351 CGGGCACAAA TTCGTCAACG GCATCCTCGA CAAACTCGCC GCCCAAATCC 401 GCCCAGACGA GCCCAAACGC CGTTGA This corresponds to the amino acid sequence <SEQ ID 2214; ORF 674.ng>: g674.pep 1 MKTARRRSRE LAVQAVYQSL IΝRTAAPEIA KΝIREMSDFA KADEELFΝKL 51 FFGTQTΝAAD YIQKIRPLLD RDEKDLΝPIE RAVLLTACHE LSAMPETPYP 101 VIIΝEAIEVT KTFGGTDGHK FVΝGILDKLA AQIRPDEPKR R* The following partial DΝA sequence was identified in N meningitidis <SEQ LD 2215>: m674. seq 1 ATGAAAACAG CCCGCCGCCG TTCCCGCGAG CTTGCCGTAC AAGCCGTTTA 51 CCAATCCCTT ATCAACCGCA CCGCCGCGCC CGAAATTGCT AAAAACATCC 101 GCGAAATGTC CGACTTTGCC AAGGCAGACG AAGAATTGTT CAACAAACTT 151 TTCTTCGGCA CGCAAACCAA TGCGGCAGAG TATATCCGAC AAATCCGCCC 201 GCTACTTGAC AGGGACGAAA AAGACCTCAA CCCCATCGAA CGCGCCGTTT 251 TGCTGACCGC CTGCCACGAG CTGTCCGCCA TGCCCGAAAC GCCCTACCCC 301 GTCATTATCA ACGAAGCCAT CGAAGTTACC AAAACCTTCG GCGGCACGGA 351 CGGGCACAAA TTCGTCAACG GCATCCTCGA CAAACTCGCC GCCCAAATCC 401 GCCCCGACGA GCCCAAACGC CGTTGA This corresponds to the amino acid sequence <SEQ ID 2216; ORF 674>: m674.pep 1 MKTARRRSRE LAVQAVYQSL IΝRTAAPEIA KΝIREMSDFA KADEELFΝKL 51 FFGTQTNAAE YIRQIRPLLD RDEKDLNPIE RAVLLTACHE LSAMPETPYP 101 VIINEAIEVT KTFGGTDGHK FVNGILDKLA AQIRPDEPKR R* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m674/g674 97.9% identity in 141 aa overlap 10 20 30 40 50 60 m674.pep MKTARRRSRELAVQAVYQSLIΝRTAAPEIAKΝIREMSDFAKADEELFΝKLFFGTQTΝAAE II II II II II I II II I I II II II II II II I II II II II I II II II II I I II II II II I I : g674 MKTARRRSRELAVQAVYQSLIΝRTAAPEIAKΝIREMSDFAKADEELFΝKLFFGTQTΝAAD 10 20 30 40 50 60 70 80 90 100 110 120 m674.pep YIRQIRPLLDRDEKDLΝPIERAVLLTACHELSAMPETPYPVIIΝEAIEVTKTFGGTDGHK I MM I II II II II II II II II I II II I II II I I I II I I II II II II II I M II II II II g674 YIQKIRPLLDRDEKDLΝPIERAVLLTACHELSAMPETPYPVIIΝEAIEVTKTFGGTDGHK 70 80 90 100 110 120 130 140 m674. pep FVΝGILDKLAAQIRPDEPKRRX II II II II II II II II I II I II g674 FVΝGILDKLAAQIRPDEPKRRX 130 140 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2217>: a674. seq 1 ATGAAAACAG CCCGCCGCCG TTCCCGCGAG CTTGCCGTAC AAGCCGTTTA 51 CCAATCCCTT ATCAACCGCA CCGCCGCGCC CGAGATTGCT AAAAACATCC 101 GCGAAATGCC CGACTTTGCC AAGGCAGACG AAGAATTGTT CAACAAACTT 151 TTCTTCGGCA CGCAAACCAA TGCGGCAGAG TACATCCGAC AAATCCGCCC 201 CCTGCTCGAC CGCGACGAAA AAGACCTCAA CCCCATCGAA CGCGCCGTCC 251 TGCTGACCGC CTGCCACGAG CTGTCCGCCA TGCCCGAAAC GCCCTACCCC 301 GTCATCATCA ACGAAGCCAT CGAAGTAACC AAAACCTTCG GCGGCACGGA 351 CGGGCACAAA TTCGTCAACG GCATCCTCGA CAAACTCGCC GCCCAAATCC 401 GTCCCGACGA GCCCAAACGC CGTTGA This corresponds to the amino acid sequence <SEQ ID 2218; ORF 674.a>: a674.pep 1 MKTARRRSRE LAVQAVYQSL IΝRTAAPEIA KΝIREMPDFA KADEELFΝKL 51 FFGTQTNAAE YIRQIRPLLD RDEKDLNPIE RAVLLTACHE LSAMPETPYP 101 VIINEAIEVT KTFGGTDGHK FVNGILDKLA AQIRPDEPKR R* m674/a674 99.3% identity in 141 aa overlap 10 20 30 40 50 60 m674.pep MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMSDFAKADEELFNKLFFGTQTNAAE II II II II II I II II II I I I I I II I I I I II II II II I II II II II II II II II II II II a674 MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMPDFAKADEELFNKLFFGTQTNAAE 10 20 30 40 50 60 70 80 90 100 110 120 m674.pep YIRQIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVIINEAIEVTKTFGGTDGHK I II II II I I I II II II II II I II I II II I II II I I I I II I I II II I II II II I II II II I a67 YIRQIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVIINEAIEVTKTFGGTDGHK 70 80 90 100 110 120 130 140 m674.pep FVNGILDKLAAQIRPDEPKRRX I II I II II II II II II II II I I a674 FVNGILDKLAAQIRPDEPKRRX 130 140 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 2219>: g675. seq 1 ATGAACACCA TCGCCCCcaa cctcgacgGC AAACACCTCC GCATCGGCAT 51 CGTACAGGCA CGCTTCACCA ACGAAATCGG CAGCCAAATG CTCAAAGTCT 101 GCTGCCGCAC CCTCCAAGAA TTGGGCGTAG CAGACGAAAa catcaccgtc 151 gCCACCGTAC CCGGCGCGCT TGAAATCCCC ATCGCGCTGA TGAACTTTGC 201 CTCTTCCGAA AAATTTGACG CACTGATTGC CATCGGCGTC GTCATCCGTG 251 GCGAAACCTA CCATTTCGAG CTGGTTGCCA ACGAATCCGG CGCAGGGATC 301 GGCCGCGTCG CACTCGACTA CAACATCCCG ATTGCCAACG CCGTCCTGAC 351 CACCGAAAAC GACGCGCAGG CAATTGAACG GATTGGAGAA AAAGCCTCGG 401 ATGCCGCCAA AGTCGCCGTA GAATGCGCCA ACCTCGTCAA CCTTCTGCTC 451 GAAGAACAGT TTGAAGACGA AGAATAA This corresponds to the amino acid sequence <SEQ ID 2220; ORF 675. ng>: g675. pep 1 MNTIAPNLDG KHLRIGIVQA RFTNEIGSQM LKVCCRTLQE LGVADENITV 51 ATVPGALEIP IALMNFASSE KFDALIAIGV VIRGETYHFE LVANESGAGI 101 GRVALDYNIP IANAVLTTEN DAQAIERIGE KASDAAKVAV ECANLVNLLL 151 EEQFEDEE* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2221>: m675.seq 1 ATGAACACCA TCGCCCCCAA CCTCGACGGC AAACACCTCC GCATCGGCAT 51 CGTACAGGCA CGCTTCACCA ACGAAATCGG CAGCGAAATG CTCAAAGTCT 101 GCTGCCGCAC CCTCCAAGAA TTGGGCGTGG CAGACGAAAA CATTACCGTC 151 GCCACCGTAC CCGGCGCGCT TGAAATCCCC ATCGCGCTGA TGAACTTTGC 201 CTCTTCCGAA AAGTTTGACG CACTGATTGC CATCGGCGTC GTCATCCGTG 251 GCGAAACCTA CCATTTCGAG CTGGTTTCCA ACGAATCCGG AGCAGGCGTC 301 AGCCGCGTCG CACTCGACTA CAATATCCCG ATTGCCAATG CCGTCCTAAC 351 CACCGAAAAC GACGCGCAGG CAATCGAACG GATTGAAGAA AAAGCCTCGG 401 ATGCCGCCAA AGTCGCCGTC GAATGCGCCA ACCTCGTCAA CCTGCTGCTC 451 GAAGAACAGT TTGAAGACGA AGAATAA This corresponds to the amino acid sequence <SEQ ID 2222; ORF 675>: m675.pep 1 MΝTIAPΝLDG KHLRIGIVQA RFTΝEIGSEM LKVCCRTLQE LGVADENITV 51 ATVPGALEIP IALMNFASSE KFDALIAIGV VIRGETYHFE LVSNESGAGV 101 SRVALDYNIP IANAVLTTEN DAQAIERIEE KASDAAKVAV ECANLVNLLL ' 151 EEQFEDEE* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m675/g675 96.8% identity in 158 aa overlap 10 20 30 40 50 60 m675.pep MΝTIAPΝLDGKHLRIGIVQARFTΝEIGSEMLKVCCRTLQELGVADEΝITVATVPGALEIP I II II II I I II II II I I II II II II I II : II I I II II I II II I I I II II II II II II I II g675 MΝTIAPΝLDGKHLRIGIVQARFTΝEIGSQMLKVCCRTLQELGVADEΝITVATVPGALEIP 10 20 30 40 50 60 70 80 90 100 110 120 m675.pep IALMΝFASSEKFDALIAIG IRGETYHFELVSΝESGAGVSRVALDYΝIPIAΝAVLTTEΝ II II I II II II II II II II II II I I II I I II I : II I I I I :: II II II II II I II II II II g675 IALMΝFASSEKFDALIAIGWIRGETYHFELVAΝESGAGIGRVALDYΝIPIAΝAVLTTEΝ 70 80 90 100 110 120 130 140 150 159 m675. pep DAQAIERIEEKASDAAKVAVECAΝLVΝLLLEEQFEDEEX II I II II I I I I II II II II II II II I II II I I I II II I g675 DAQAIERIGEKASDAAKVAVECAΝLVΝLLLEEQFEDEEX 130 140 150 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2223>: a675.seq 1 ATGAACACCA TCGCCCCCAA CCTCGACGGC AAACACCTCC GCATCGGCAT 51 CGTACAGGCA CGCTTCACCA ACGAAATCGG CAGCGAAATG CTCAAAGTCT 101 GCTGCCGCAC CCTCCAAGAA TTGGGCGTGG CAGACGAAAA CATTACCGTC 151 GCCACCGTAC CCGGCGCGCT TGAAATCCCC ATCGCGCTGA TGAACTTTGC 201 CTCTTCTGAA AAATTTGACG CACTGATTGC CATCGGCGTC GTTATCCGTG 251 GCGAAACCTA CCATTTCGAG CTGGTTTCCA ACGAATCCGG AGCAGGGGTC 301 AGCCGCGTCG CACTCGACTA CAACATCCCG ATTGCCAATG CCGTCCTGAC 351 CACGGAAAAC GACGCACAGG CAATCGAACG GATTGAAGAA AAAGCCTCGG 401 ATGCCGCCAA AGTCGCCGTA GAATGCGCCA ACCTCGTCAA CCTCCTGCTC 451 GAAGAACAGT TTGAAGACGA AGAATAA This corresponds to the amino acid sequence <SEQ ID 2224; ORF 675. a>: a675.pep 1 MΝTIAPΝLDG KHLRIGIVQA RFTΝEIGSEM LKVCCRTLQE LGVADENITV 51 ATVPGALEIP IALMNFASSE KFDALIAIGV VIRGETYHFE LVSNESGAGV 101 SRVALDYNIP IANAVLTTEN DAQAIERIEE KASDAAKVAV ECANLVNLLL 151 EEQFEDEE* m675/a675 100.0% identity in 158 aa overlap 10 20 30 40 50 60 m675. pep MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVADENITVATVPGALEIP II II II I II II II I II II II II II I II II I II I II I II I II II II II II I II II II II I I a675 MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVADENITVATVPGALEIP 10 20 30 40 50 60 70 80 90 100 110 120 m675 . pep IALMNFASSEKFDALIAIGWIRGETYHFELVSNESGAGVSRVALDYNIPIANAVLTTEN M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M a675 IALMNFASSEKFDALIAIGWIRGETYHFELVSNESGAGVSRVALDYNIPIANAVLTTEN 70 80 90 100 110 120 130 140 150 159 m675.pep DAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEEX II II II II II II II II II II II I II II II II II II I II I a675 DAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEEX 130 140 150 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2225>: g677. seq 1 ATGCCGCAGA TTTTGGTGCG GATTTTCCTC ATTCGGTATT CCTTTATTtg 51 ggAAACGGTG CGCTTGTGCC GTTTCAGACG GCATTCCCGA TCAGTCGATT 101 TTGATGTATT CGACAGAAAG GATTTCAATT TCCTCACGGC CTTCCGGCGT 151 GTTCAAAACC ACTTCGTCGC CTTCGCGCGC TTTAATCAGG CAACGCGCCA 201 ACGGCGAAAT CCAAGAAATT TTGTTTTGCG CGGTATCGAT TTCATCGACG 251 CCGACGATTT TGACGGTTTG CTCGCGCCCG TCGCCGCGCA ACAGACCGAC 301 GGTCGCGCCG AAAAATACTT GGTCGGTCGC TTCGCGCAAT TCGGGATCGA 351 CGACGACGGC AGCCTCCAAA CGTTTGGTCA GGAAACGGAT GCGGCGGTCG 401 ATTTCGCGCA TACGGCGTTT GCCGTAAAGA TAGTCGCCGT TTTCGCTGCG 451 GTCGCCGTTG CCTGCCGCCC AGTTGACGAT TTGGACGATT TCGGGGCGTT 501 CTTTATTGAC CAGTTGATAA AGCTCGTCTT TCAATGCCTG CCATCCGGCG 551 GGCGTAATGT AGTTTTTGGT TTCGGTACTC ATATTGTGTG CGGATGA This corresponds to the amino acid sequence <SEQ ID 2226; ORF 677.ng>: g677.pep 1 MPQILVRIFL IRYSFIWETV RLCRFRRHSR SVDFDVFDRK DFΝFLTAFRR 51 VQΝHFVAFAR FΝQATRQRRΝ PRΝFVLRGID FIDADDFDGL LAPVAAQQTD 101 GRAEKYLVGR FAQFGIDDDG SLQTFGQETD AAVDFAHTAF AVKIVAVFAA 151 VAVACRPVDD LDDFGAFFID QLIKLVFQCL PSGGRΝWFG FGTHIVCG* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2227>: Hi677. seq 1 ATGCCGCAGA TTTTGGTGCG GATTTTCCTC ATTCGGTATT CCTTTATTTG 51 GGAAACGGCG CGCTTTTGCC GTTTCAGACG GCATTCCCGA TCAGTCGATT 101 TTGATGTATT CGACAGAAAG GATTTCAATT TCCTCACGCC CTTCCGGCGT 151 GTTCAAAACC ACTTCGTCGC CTTCGCGCGC TTTAATCAGA CAACGAGCCA 201 GCGGCGAAAT CCAAGAAATT TTGTTTTGCG CGGTATCGAT TTCATCGATG 251 CCGACGATTT TGACGGTTTG CTCGCGCCCG TCGTCGCGCA ACAGTCCGAC 301 CGTCGCGCCG AAAAACACTT GGTCGGTCGC TTCGCGCAAT TCGGGATCGA 351 CGACGACGGC AGCCTCCAAA CGTTTGGTCA GGAAACGGAT GCGGCGGTCG 401 ATTTCGCGCA TACGGCGTTT GCCGTAAAGA TAGTCGCCGT TTTCGCTGCG 451 GTCGCCGTTG CCTGCCGCCC AGTTGACGAT TTGGACGATT TCGGGGCGTT 501 CTTTGTTGAC CAGTTGATAA AGCTCGTCTT TCAATGCCTG CCATCCGGCG 551 GGCGTAATGT AGTTTTTGGT TTCGGTACTC ATATTGTGTG CGGATGA This corresponds to the amino acid sequence <SEQ ID 2228; ORF 677>: m677.pep 1 MPQILVRIFL IRYSFIWETA RFCRFRRHSR SVDFDVFDRK DFΝFLTPFRR 51 VQNHFVAFAR FNQTTSQRRN PRNFVLRGID FIDADDFDGL LAPWAQQSD 101 RRAEKHLVGR FAQFGIDDDG SLQTFGQETD AAVDFAHTAF AVKIVAVFAA 151 VAVACRPVDD LDDFGAFFVD QLIKLVFQCL PSGGRNWFG FGTHIVCG* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m677/g677 94.9% identity in 198 aa overlap 10 20 30 40 50 60 m677.pep MPQILVRIFLIRYSFIWETARFCRFRRHSRSVDFDVFDRKDFΝFLTPFRRVQΝHFVAFAR II II II II I II II II II II : I: II II II I II II II II I II II II II II II II II II II I g677 MPQILVRIFLIRYSFIWETVRLCRFRRHSRSVDFDVFDRKDFΝFLTAFRRVQΝHFVAFAR 10 20 30 40 50 60 70 80 90 100 110 120 m677.pep FΝQTTSQRRΝPRΝFVLRGIDFIDADDFDGLLAPWAQQSDRRAEKHLVGRFAQFGIDDDG IMM I I II II II II I I I I II II II II II II II : I I I : I MMMMMMMMM g677 FΝQATRQRRΝPRΝFVLRGIDFIDADDFDGLLAPVAAQQTDGRAEKYLVGRFAQFGIDDDG 70 80 90 100 110 120 130 140 150 160 170 180 m677. pep SLQTFGQETDAAVDFAHTAFAVKIVAVFAAVAVACRPVDDLDDFGAFFVDQLIKLVFQCL II II II II II II II II I II I I II II II I I II I I II II II II II II II I : II I II II I I II g677 SLQTFGQETDAAVDFAHTAFAVKIVAVFAAVAVACRPVDDLDDFGAFFIDQLIKLVFQCL 130 140 150 160 170 180 190 199 m677.pep PSGGRΝWFGFGTHIVCGX II I II II II II II II II II g677 PSGGRΝWFGFGTHIVCGX 190 The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 2229>: a677. seq 1 ATGCCGCAGA TTTTGGTGCG GATTTTCCTC ATTCGGTATT CCTTTATTTG 51 GGAAACGGCG CGTTTGTGCC GTTTCAGACG GCATTCCCGA TCAGTCGATT 101 TTGATGTATT CGACAGAAAG GATTTCAATT TCCTCACGCC CTTCCGGCGT 151 GTTTAAAACC ACTTCGTCGC CTTCACGCGC TTTAATCAGA CAACGAGCCA 201 GCGGCGAAAT CCAAGAAATT TTGTTTTGCG CGGTATCGAT TTCATCGATG 251 CCGACGATTT TGACGGTTTG CTCGCGCCCG TCGCCGCGCA ACAGACCGAC 301 GGTCGCGCCG AAAAACACTT GGTCGGTCGC TTCGCGCAAT TCGGGATCAA 351 CGACGACGGC GGCTTCCAAA CGCTTGGTCA GGAAACGGAT GCGGCGGTCG 401 ATTTCGCGCA TACGGCGTTT GCCGTAAAGG TAGTCGCCGT TTTCGCTGCG 451 GTCGCCGTTG CCTGCCGCCC AGTTGACGAT TTGGACGATT TCGGGGCGTT 501 CTTTATTAAC CAGTTGATAA AGCTCGTCTT TCAATGCCTG CCATCCGGCG 551 GGCGTAATGT AGTTTTTGGT TTCGGTACTC ATATTGTGTG CGGATGA This corresponds to the amino acid sequence <SEQ ID 2230; ORF 677. a>: a677.pep 1 MPQILVRIFL IRYSFIWETA RLCRFRRHSR SVDFDVFDRK DFΝFLTPFRR 51 V*ΝHFVAFTR FNQTTSQRRN PRNFVLRGID FIDADDFDGL LAPVAAQQTD 101 GRAEKHLVGR FAQFGINDDG GFQTLGQETD AAVDFAHTAF AVKWAVFAA 151 VAVACRPVDD LDDFGAFFIN QLIKLVFQCL PSGGRNWFG FGTHIVCG* m677/a677 93.4% identity in 198 aa overlap 10 20 30 40 50 60 m677.pep MPQILVRIFLIRYSFIWETARFCRFRRHSRSVDFDVFDRKDFNFLTPFRRVQNHFVAFAR II I I I II II II I II II I I II I : II II I I II II II I II II II II II II II II IIIIIMI a677 MPQILVRIFLIRYSFIWETARLCRFRRHSRSVDFDVFDRKDFNFLTPFRRVXNHFVAFTR 10 20 30 40 50 60 70 80 90 100 110 120 m677.pep FNQTTSQRRNPRNFVLRGIDFIDADDFDGLLAPWAQQSDRRAEKHLVGRFAQFGIDDDG II II II I II I I II II II I I I II I II II II II II I : I II : I I II I II II II II II I : I II a677 FNQTTSQRRNPRNFVLRGIDFIDADDFDGLLAPVAAQQTDGRAEKHLVGRFAQFGINDDG 70 80 90 100 110 120 130 140 150 160 170 180 m677. pep SLQTFGQETDAAVDFAHTAFAVKIVAVFAAVAVACRPVDDLDDFGAFFVDQLIKLVFQCL : : I I : I II I II II I II I I II II I : I II I II II II II II II I II II II I :: I II II I II II a677 GFQTLGQETDAAVDFAHTAFAVKWAVFAAVAVACRPVDDLDDFGAFFINQLIKLVFQCL 130 140 150 160 170 180 190 199 m677.pep PSGGRNWFGFGTHIVCGX I II II I II II II II II II I a677 PSGGRNWFGFGTHIVCGX 190 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2231>: g678.seq 1 ATGAATAGCC TCCCCATTGC CGACCTCCTC GCCTccgCCG TCATCGCCGC 51 CTGCATCGTC ATTTCCACGA TGCGCGGCGT GATTGCGGAA GCAggttcGA 101 TGGTgGCATG ggtggTTTcc tTCTTTTttg ccAAACTCTt tGCCGCACcc 151 ttcgccgACC TCGCCTTTGc ctCGTTCCAA ccccgccTGT TTGCAttggc 201 tCTGTCATTC ATTTCCCTGT TCGTCATTGC CTGTCTGATC CAGAAAATGC 251 TCCGTTCGCT GCTGACCGGC GCAGTTTCGG CGGTCGGTCT GGGCTTTGCC 301 AACCGCATTT TGGGCGGTGT ATTCGGTGCA TTGAAAGGCG TTTTGATTGT 351 TACCCTGCTG ATCATGCTTG CTTCAAAAAC CGACCTGCCC GATACCGAAG 401 AATGGCAACA GTCCTATACC GTACCGTTTT TCGTATCGCT TTCCGAAGCG 451 GTGTTAAACC atacggaCAA CGCacccgaa tCCCtcgacg acgactaa This corresponds to the amino acid sequence <SEQ ID 2232; ORF 678.ng>: g678.pep 1 MΝSLPIADLL ASAVIAACIV ISTMRGVIAE AGSMVAWWS FFFAKLFAAP 51 FADLAFASFQ PRLFALALSF ISLFVIACLI QKMLRSLLTG AVSAVGLGFA 101 ΝRILGGVFGA LKGVLIVTLL IMLASKTDLP DTEEWQQSYT VPFFVSLSEA 151 VLΝHTDΝAPE SLDDD* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2233>: m678. seq 1 ATGAATAGCC TCCCCATTGC CGACCTCCTC GTCTCCGCCG TCATCGCCGC 51 CTGCATCGTG CTATCCGCGA TGCGCGGCGT GATTGCGGAG GCAGGCTCAA 101 TGGCGGCATG GGTGGTTTCC TTCTTTTTCG CCAAACTCTT TGCCGCCTCC 151 TTCGCCGACC TCGCCTTTGC CTCGTTCCAA CCCCGCCTGT TTGCATTGGC 201 TCTGTCGTTC ATTTCCCTGT TCGTCATTGC CTGTCTGATC CAGAAAATGC 251 TCCGTTCGCT GCTGACCAGC GCAGTTTCGG CGGTCGGTTT GGGCTTTGCC 301 AACCGCATTT TGGGCGGCGT ATTCGGTGCA TTGAAAGGCG TTTTGATTGT 351 TACCCTGCTG GTCATGCTTG CTTCAAAAAC CGACCTGCCC GATACCGAAG 401 AATGGCGGCA ATCTTACACA CTGCCGTTTT TCGTATCGCT TTCCGAAGCC 451 GTGTTGAACC ATAGCGGCGG CACGGCGGAA ACTCCGGAAG ACGATTGA This corresponds to the amino acid sequence <SEQ ID 2234; ORF 678>: m678.pep 1 MΝSLPIADLL VSAVIAACIV LSAMRGVIAE AGSMAAWWS FFFAKLFAAS 51 FADLAFASFQ PRLFALALSF ISLFVIACLI QKMLRSLLTS AVSAVGLGFA 101 ΝRILGGVFGA LKGVLIVTLL VMLASKTDLP DTEEWRQSYT LPFFVSLSEA 151 VLΝHSGGTAE TPEDD* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m678/g678 89.7% identity in 165 aa overlap 10 20 30 40 50 60 m678, ■pep MNSLPIADLLVSAVIAACIVLSAMRGVIAEAGSMAAWWSFFFAKLFAASFADLAFASFQ II I I I II II I : II II II II I : I : I II II I I I II I : I I II I II II II II I II I II I II I I g678 MNSLPIADLLASAVIAACIVISTMRGVIAEAGSMVAWWSFFFAKLFAAPFADLAFASFQ 10 20 30 40 50 60 70 80 90 100 110 120 m678, • pep PRLFALALSFISLFVIACLIQKMLRSLLTSAVSAVGLGFANRILGGVFGALKGVLIVTLL II I I II II I II II II II II II II II II II : I II II II II I I II II II II II II I II II II g678 PRLFALALSFISLFVIACLIQKMLRSLLTGAVSAVGLGFANRILGGVFGALKGVLIVTLL 70 80 90 100 110 120 130 140 150 160 m678. pep VMLASKTDLPDTEEWRQSYTLPFFVSLSEAVLNHSGGTAETPEDDX : II II II II I II II I : II II : II II II II II II I : : : M MM g678 IMLASKTDLPDTEEWQQSYTVPFFVSLSEAVLNHTDNAPESLDDDX 130 140 150 160 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2235>: a678. seq 1 ATGAATAACC TCCCCGTTGC CGACCTCCTC GTCTCCGCCA TCATCGCCGC 51 CTGCATCGTG CTATCCGCGA TGCGCGGCGT GATTGCGGAG GCTGGCTCAA 101 TGGCGGCATG GGTGGTTGCC TTTTTTTTCG CCAAACTCTT TGCCGCACCC 151 TTCGCCGACA TCGCCTTTGC ATCGTTCCAA CCCCGCCTGT TTGCATTGGC 201 TCTGTCGTTC ATTTCCCTAT TCGTCATTGC CTGTCTGATC CAGAAAATAC 251 TCCGCTCGCT GCTGACCGGG GCAGTTTCGG CGGTCGGTTT GGGCTTTGCC 301 AACCGCATTT TGGGCGGCGT ATTCGGTGCA TTGAAAGGCA TTTTGATTAT 351 TACCCTGCTG GTCATGCTCG CTTCAAAAAC CGACCTGCCC GATACCGAAG 401 AATGGCGGCA ATCTTACACA CTGCCGTTTT TCGTATCGCT TTCCGAAGCC 451 GTGTTGAACC ATAGCGGCGG CACGGCGGAA ACTCCGGAAG ACGATTGA This corresponds to the amino acid sequence <SEQ LD 2236; ORF 678. a>: a678.pep 1 MΝΝLPVADLL VSAIIAACIV LSAMRGVIAE AGSMAAWWA FFFAKLFAAP 51 FADIAFASFQ PRLFALALSF ISLFVIACLI QKILRSLLTG AVSAVGLGFA 101 ΝRILGGVFGA LKGILIITLL VMLASKTDLP DTEEWRQSYT LPFFVSLSEA 151 VLΝHSGGTAE TPEDD* m678/a678 93.9% identity in 165 aa overlap 10 20 30 40 50 60 m678.pep MΝSLPIADLLVSAVIAACIVLSAMRGVIAEAGSMAAWVVSFFFAKLFAASFADLAFASFQ II : I I : I II I II M I II II II II I II II I II II II II I I : II I I I II I I I I I : II I II I a678 MΝΝLPVADLLVSAIIAACIVLSAMRGVIAEAGSMAAWVVAFFFAKLFAAPFADIAFASFQ 10 20 30 40 50 60 70 80 90 100 110 120 m678.pep PRLFALALSFISLFVIACLIQKMLRSLLTSAVSAVGLGFAΝRILGGVFGALKGVLIVTLL II II I II II I II II II I I II II : I II I I I: II II I I I I II II II II II II II I : II: II I a678 PRLFALALSFISLFVIACLIQKILRSLLTGAVSAVGLGFAΝRILGGVFGALKGILIITLL 70 80 90 100 110 120 130 140 150 160 m678.pep VMLASKTDLPDTEEWRQSYTLPFFVSLSEAVLΝHSGGTAETPEDDX II I II II I II II II I II II II I II I II II II II II II I I II I I I I I a678 VMLASKTDLPDTEEWRQSYTLPFFVSLSEAVLΝHSGGTAETPEDDX 130 140 150 160 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 2237>: g680.seq 1 ATGACGAAGG GCAGTTCGGC GATGTCCAGC CCACGCGCGG CGATATCGGT 51 GGCGACGAGG ACGCGCAGGC TGCCGTCTTT GAAGGCGTTG AGTGTTTCGA 101 GCCTGCTTTG TTGGGAACGG TCGCCGTGTA TCGCCTGTGC GGACAGGTTG 151 CGGCGCACCA GTTCGCGCGT TACGCGGTCG ACGCTTTGTT TGGTtttgCA 201 AAAGACGATA ACTTGGTTCA TATGCAGATC GACAATCAGC CGTTCGAGCA 251 GGTTGCGCTT TTGGAAGGTA TCGACGGCGA TGATGTgttg ttcGACGTTG 301 GCGTTGGTGG TGTTTTGGGC GGCAACCTCG ACGGTTTCGG GCGCGTTCAT 351 GAAGTCTTGC GCCAGTTTGC GTATCGGTGC GGAGAAGGTG GCGGAAAAGA 401 GCAGGGTTTG GCGTTGGCGG GGCAGCATCT GCATGATTTT GCGGATGTCG 451 TCGATAAACC CCATATCCAA CATGCGGTCT GCTTCGTCCA GAACGACGAT 501 TTCGGCTTTG TTTAAACTGA TGTTTTTCTG TTTCACATGG TCGAGCAGCC 551 GTCCGACGGT GGCGACGACT ATTTCGCAGC CGGCACGCAG GTCGGCGGTT 601 TGTTTGTCCA TGTTGACACC GCCGAAGAGG ACGGTATGCC GCAGCGGCAG 651 GTTTTTAATg tag This corresponds to the amino acid sequence <SEQ ID 2238; ORF 680.ng>: g680.pep 1 MTKGSSAMSS PRAAISVATR TRRLPSLKAL SVSSLLCWER SPCIACADRL 51 RRTSSRVTRS TLCLVLQKTI TWFICRSTIS RSSRLRFWKV STAMMCCSTL 101 ALWFWAATS TVSGAFMKSC ASLRIGAEKV AEKSRVWRWR GSICMILRMS 151 SINPISNMRS ASSRTTISAL FKLMFFCFTW SSSRPTVATT ISQPARRSAV 201 CLSMLTPPKR TVCRSGRFLM * The following partial DNA sequence was identified in N meningitidis <SEQ ID 2239>: m680. seq 1 ATGACGAAGG GCAGTTCGGC AATGTCCAGC CCGCGCGCGG CGATGTCGGT 51 GGCGACGAGG ACGCGCAGGT TGCCGTCTTT GAAGGCGTTG AGTGTTTCGA 101 GCCGGCTTTG TTGGGAACGG TCGCCGTGTA TCGCCTGTGC GGACAGGTTG 151 CGGCGCACCA GTTCGCGCGT TACGCGGTCG ACGCTTTGTT TGGTTTTGCA 201 GAACACGATG ACCTGGTTCA TATGCAAATC GACAATCAGC CGTTCGAGCA 251 GGTTGCGCTT CTGAATGGTA TCGACGGCGA TGATGTGCTG CTCGACGTTG 301 GCGTTGGTGG TGTTTTGCGC GGCGACTTCG ACGGTTTCGG GCGCGTTCAT 351 GAAGTCTTGC GCCAGTTTGC GTATCGGGGC GGAGAAGGTG GCGGAAAAGA 401 GCAGGGTTTG GCGTTGGCGG GGCAGCATCT GCATGATTTT GCGGATGTCG 451 TCGATAAAAC CCATATCCAG CATACGGTCG GCTTCGTCCA AAACGACGAT 501 TTCGACTTTG TTCAAATGGA TGTTTTTCTG TTTCACGTGG TCGAGCAGCC 551 GTCCGACGGT GGCGACGACG ATTTCGCAGC CGGCACGCAG GTCGGCGGTC 601 TGTTTGTCCA TATTCATACC GCCGAACAAG ACGGTGTGGC GCAGCGGCAG 651 GTTTTTGATG TAG This corresponds to the amino acid sequence <SEQ ID 2240; ORF 680>: m680.pep 1 MTKGSSAMSS PRAAMSVATR TRRLPSLKAL SVSSRLCWER SPCIACADRL 51 RRTSSRVTRS TLCLVLQΝTM TWFICKSTIS RSSRLRF*MV STAMMCCSTL 101 ALWFCAATS TVSGAFMKSC ASLRIGAEKV AEKSRVWRWR GSICMILRMS 151 SIKPISSIRS ASSKTTISTL FKWMFFCFTW SSSRPTVATT ISQPARRSAV 201 CLSIFIPPΝK TVWRSGRFLM * Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m680/g680 90.9% identity in 220 aa overlap 10 20 30 40 50 60 m680.pep MTKGSSAMSSPRAAMSVATRTRRLPSLKALSVSSRLCWERSPCIACADRLRRTSSRVTRS II II II II I II II M II II II II II II II II I I I I I II II II II I II II II I I II II II g680 MTKGSSAMSSPRAAISVATRTRRLPSLKALSVSSLLCWERSPCIACADRLRRTSSRVTRS 10 20 30 40 50 60 70 80 90 100 110 120 m680. pep TLCLVLQΝTMTWFICKSTISRSSRLRFXMVSTAMMCCSTLAL FCAATSTVSGAFMKSC II II II I : I M II II M II II II II II II I II I II II I II I II II II I II I I II I II g680 TLCLVLQKTITWFICRSTISRSSRLRFWKVSTAMMCCSTLALWFWAATSTVSGAFMKSC 70 80 90 100 110 120 130 140 150 160 170 180 m680.pep ASLRIGAEKVAEKSRVWRWRGSICMILRMSSIKPISSIRSASSKTTISTLFKWMFFCFTW I II II II II II II II II II II II II II II II I : II I : : I II I I : I II I : II I g680 ASLRIGAEKVAEKSRVWRWRGSICMILRMSSINPISNMRSASSRTTISALFKLMFFCFTW 130 140 150 160 170 180 190 200 210 220 m680. pep SSSRPTVATTISQPARRSAVCLSIFIPPNKTVWRSGRFLMX MMMMMMMMMMMM: IMM! II I II I II g680 SSSRPTVATTISQPARRSAVCLSMLTPPKRTVCRSGRFLMX 190 200 210 220 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2241>: a680.seq 1 ATGACGAAGG GCAGTTCGGC AATATCCAGC CCCCGCGCGG CGATATCGGT 51 GGCGACGAGG ACGCGCAGGT TGCCGTCTTT GAAGGCGTTG AGTGTTTCGA 101 GCCGGCTTTG TTGGGAACGG TCGCCGTGTA TCGCCTGTGC GGACAGGTTG 151 CGGCGCACCA GTTCGCGCGT TACGCGGTCG ACGCTTTGTT TGGTTTTGCA 201 GAACACGATG ACCTGGTTCA TATGCAAATC GACAATCAGC CGTTCGAGCA 251 GGTTGCGCTT CTGAATGGTA TCGACGGCGA TGATGTGCTG CTCGACGTTG 301 GCGTTGGTGG TGTCTTGCGC GGCGACTTCG ACGGTTTCGG GCGCGTTCAT 351 GAAGTCTTGC GCCAGTTTGC GTATCGGGGC GGAGAAGGTG GCGGAAAAGA 401 GCAGGGTTTG GCGTTGGCGG GGCAGCATCT GCATGATTTT GCGGATGTCG 451 TCGATAAAAC CCATATCCAG CATACGGTCG GCTTCGTCCA AAACGACGAT 501 TTCGACTTTG TTCAAATGGA TGTTTTTCTG TTTCACGTGG TCGAGCAGCC 551 GTCCGACGGT GGCGACGACG ATTTCGCAGC CGGCACGCAG GTCGGCGGTC 601 TGTTTGTCCA TATTCATACC GCCGAACAAG ACGGTGTGGC GCAGCGGCAG 651 GTTTTTGATG TAG This corresponds to the amino acid sequence <SEQ ID 2242; ORF 680.a>: a680.pep 1 MTKGSSAISS PRAAISVATR TRRLPSLKAL SVSSRLCWER SPCIACADRL 51 RRTSSRVTRS TLCLVLQΝTM TWFICKSTIS RSSRLRF*MV STAMMCCSTL 101 ALWSCAATS TVSGAFMKSC ASLRIGAEKV AEKSRVWRWR GSICMILRMS 151 SIKPISSIRS ASSKTTISTL FKWMFFCFTW SSSRPTVATT ISQPARRSAV 201 CLSIFIPPΝK TVWRSGRFLM * m680/a680 98.6% identity in 220 aa overlap 10 20 30 40 50 60 m680.pep MTKGSSAMSSPRAAMSVATRTRRLPSLKALSVSSRLCWERSPCIACADRLRRTSSRVTRS I II II II : I II II I : II II II II II I II I II II II II II II I I II II II I II II II II I I a680 MTKGSSAISSPRAAISVATRTRRLPSLKALSVSSRLCWERSPCIACADRLRRTSSRVTRS 10 20 30 40 50 60 70 80 90 100 110 120 m680. pep TLCLVLQΝTMTWFICKSTISRSSRLRFXMVSTAMMCCSTLALWFCAATSTVSGAFMKSC II I I II II II I II II II II I II II II II II II II II II II II II MMMMIMMM a680 TLCLVLQΝTMTWFICKSTISRSSRLRFXMVSTAMMCCSTLALWSCAATSTVSGAFMKSC 70 80 90 100 110 120 130 140 150 160 170 180 m680.pep ASLRIGAEKVAEKSRVWRWRGSICMILRMSSIKPISSIRSASSKTTISTLFKWMFFCFTW MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a680 ASLRIGAEKVAEKSRVWRWRGSICMILRMSSIKPISSIRSASSKTTISTLFKWMFFCFTW 130 140 150 160 170 180 190 200 210 220 m680.pep SSSRPTVATTISQPARRSAVCLSIFIPPΝKTVWRSGRFLMX I I I II I I II II II I II I II II I II II II II II II II I I I II a680 SSSRPTVATTISQPARRSAVCLSIFIPPΝKTVWRSGRFLMX 190 200 210 220 The following partial DΝA sequence was identified in N. gonorrhoeae <SEQ ID 2243>: g681.seq 1 ATGACGACGC CGATGGCAAT CAGTGCGTCA AATTTTTCGG AAGAGGCAAA 51 GTTCATCAGC GCGATGGGGA TTTCAAGCGC GCCGGGTACG GTGGcgacgg 101 tgatgtTTTC GTCTGCTACG CCCAATTCTT GGAGGGTGCG GCAGCAGACT 151 TTGAGCATTT GGCTGCCGAT TTCGTTGGTG AAGCGTGCCT GTACGATGCC 201 GATGCGGAGG TGTTTGCcgt cgaggttgGG GGCGATGGTG TTCATTGGGT 251 GTCCTTTGGT ATTCGGGGTT TCGGAATGCC GTCTGAAGGT TTCAGTCTTG 301 CGGCTGCCAG TCGGCAACGG TTTGGAATGT GCCGTCTTCG GCAAGCTCCC 351 ACGCGCTGCC TTCGGGTTGG GAAAGCAGTG CGGCGGTTTC AGGGTTGGTT 401 TTGGTGATGT CGGCGAGGCT GACGATGCTG AAGTTGTCGG GGTCGTCGGT 451 GTATTCGTCG GTTTCGTCGC CGCTGAAGAA ACGCCAGCCG CTGTCGTTTT 501 CAAAAACGGG GGCTTCGCGG TAAAGGAAGC CGACGGGCCG GTTTTGTTTG 551 GCGACGGTGT TGGTGGCGAT GCAGCGGTCG AGTGCCGAGG AAAGTGCTTG 601 TGCAAATGCG TTCATTGCGG GAATACGTTG GGGGGGGGGA AACTTGCGGA 651 TTTTACCACG ATTCCCGCGT TGTCGGCAGA CGGCGGCGGT TTGGTGGTAC 701 AATGTGCGCC GTTTGCAGCC TTAAGGTGTT TCTGTATTTT TGGAGTATGG 751 AAACGCATTC GGGCTGTTTT TTGCGGAAGA CGGTAA This corresponds to the amino acid sequence <SEQ ID 2244; ORF 681>: g681.pep 1 MTTPMAISAS NFSEEAKFIS AMGISSAPGT VATVMFSSAT PNSWRVRQQT 51 LSIWLPISLV KRACTMPMRR CLPSRLGAMV FIGCPLVFGV SECRLKVSVL 101 RLPVGNGLEC AVFGKLPRAA FGLGKQCGGF RVGFGDVGEA DDAEWGWG 151 VFVGFVAAEE TPAAWFKNG GFAVKEADGP VLFGDGVGGD AAVECRGKCL 201 CKCVHCGNTL GGGKLADFTT IPALSADGGG LVVQCAPFAA LRCFCIFGVW 251 KRIRAVFCGR R* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2245>: 1 ATGACGACGC CGATGGCAAT CAGTGCGTCA AACTTTTCGG AAGAGGCAAA 51 GTTCATCAGC GCGATGGGGA TTTCAAGCGC GCCGGGTACG GTGGCGACGG 101 TAATGTTTTC GTCTGCCACG CCCAATTCTT GGAGGGTGCG GCAGCAGACT 151 TTGAGCATTT CGCTGCCGAT TTCGTTGGTG AAGCGTGCCT GTACGATGCC 201 GATGCGGAGG TGTTTGCCGT CGAGGTTGGG GGCGATGGTG TTCATTGGGT 251 GTCCTTTGGT ATTCGGAGTT TCGGAATGCC GTCTGAAGGT TTCAGTCTTG 301 CGGCTGCCAG TCGGCGACGG TTTGGAATGT GCCGTCTTCG GCAAGCTCCC 351 ATGCGCTGCC TTCGGGTTGG GAGAGCAGTG CGGCGGTTTC AGGGTTGGTT 401 TTGGCGATGT CGGCGAGGCT GACGATGCTG AAGTTGTCCG GATCGTCGGT 451 GTATTCGTCG GTCTCGTCGC CGCTGAAGAA ACGCCAGCCG CTGTCGTTTT 501 CAAAAACGGG GGCTTCGCGG TAGAGGAAGC CGACGGGCCG GTTTTGTTTG 551 GCGACGGTGT TGGTGGCGAT ACAGCGGTCG AGTGCCGAGG AAAGTGCTTG 601 TGCAAATGCG TTCATTACGG GAATACGTTG GGGG.AAAAC TTACGGATTT 651 TACCACGATT CGTGCGTTGT CGGCAGACGG CGGCGGTTTG GTGGTACAAT 701 GTGCGCCGTT TGCAGCCTTA AGGTGTTTCT GTATTTTTGG AGTATGGAAA 751 CGCATTCGGG CTGTTTTTTG CGGAAGACGG TAA This corresponds to the amino acid sequence <SEQ ID 2246; ORF 681>: m681.pep 1 MTTPMAISAS NFSEEAKFIS AMGISSAPGT VATVMFSSAT PNSWRVRQQT 51 LΞISLPISLV KRACTMPMRR CLPSRLGAMV FIGCPLVFGV SECRLKVSVL 101 RLPVGDGLEC AVFGKLPCAA FGLGEQCGGF RVGFGDVGEA DDAEWRIVG 151 VFVGLVAAEE TPAAWFKNG GFAVEEADGP VLFGDGVGGD TAVECRGKCL 201 CKCVHYGNTL GXKLTDFTTI RALSADGGGL WQCAPFAAL RCFCIFGVWK 251 RIRAVFCGRR * Computer analysis of the amino acid sequences gave the following results: Homology with a predicted ORF from N meningitidis menA with menB ORF 681 shows 94.6% identity over a 261 aa overlap with a predicted ORF (ORF681.a) from N gonorrhoeae: m681/g681 10 20 30 40 50 60 m681.pep MTTPMAISASΝFSEEAKFISAMGISSAPGTVATVMFSSATPΝSWRVRQQTLSISLPISLV I I II I II II II I I II II I II II II II II II II II II II II II I II II I II I II llllll g681 MTTPMAISASΝFSEEAKFISAMGISSAPGTVATVMFSSATPΝSWRVRQQTLSIWLPISLV 10 20 30 40 50 60 70 80 90 100 110 120 m681. pep KRACTMPMRRCLPSRLGAMVFIGCPLVFGVSECRLKVSVLRLPVGDGLECAVFGKLPCAA I II I I II II II II II II II I II II II II II II II II II II II II I : II II II II II I II g 681 KRACTMPMRRCLPSRLGAMVFIGCPLVFGVSECRLKVSVLRLPVGNGLECAVFGKLPRAA 70 80 90 100 110 120 130 140 150 160 170 180 m681. pep FGLGEQCGGFRVGFGDVGEADDAEWRIVGVFVGLVAAEETPAAVVFKNGGFAVEEADGP I II I : II II II II II II I I II II II I : II II I I : II II I II I II II II II II I : I I II I g681 FGLGKQCGGFRVGFGDVGEADDAEVVGWGVFVGFVAAEETPAAWFKNGGFAVKEADGP 130 140 150 160 170 180 190 200 210 220 230 239 m681.pep VLFGDGVGGDTAVECRGKCLCKCVHYGNTLGX-KLTDFTTIRALSADGGGLWQCAPFAA I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I : I I I I I M M M M M M M M M g681 VLFGDGVGGDAAVECRGKCLCKCVHCGNTLGGGKLADFTTIPALSADGGGLWQCAPFAA 190 200 210 220 230 240 240 250 260 m681.pep LRCFCIFGVWKRIRAVFCGRRX I II II II I I II II I II I II II I g681 LRCFCIFGVWKRIRAVFCGRRX 250 260 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2247>: a681.seq 1 ATAACGACGC CGATGGCAAT CAGTGCGTCA AATTTTTCAG AAGAGGCAAA 51 GTTCATCAGC GCGATGGGGA TTTCAAGCGC GCCGGGTACG GTGGCGACGG 101 TAATGTTTTC GTCTGCCACG CCCAATTCTT GGAGGGTGCG GCAGCAGACT 151 TTGAGCATTT CGCTGCCGAT TTCGTTGGTG AAGCGTGCCT GTACGATGCC 201 GATGCGGAGG TGTTTGCCGT CGAGGTTGGG GGCGATGGTG TTCATTGAGT 251 GTCCTTTGGT ATTCGGAGGT TTCGGAATGC CGTCTGAAGG GTCAGTCCTT 301 AGGTTGCCAG TCGGCGACGG TTTGGAATGT GCCGTCTTCT GCCAATTCCC 351 ACGCGCTGCC TTCAGGTTGG GAGAGCAGTG CGGCGGTTTC AGGGTTGGTT 401 TTGGTGATAT CGGCGAGGCT GACGATGCTG AAGTTGTCCG GGTCGTCGGT 451 GTATTCGTCG GTCTCGTCGC CGCTGAAGAA ACGCCAGCCG CTGTCGTTTT 501 CAAAAACGGG GGCTTCGCGG TAGAGGAAGC CGACGGGCTG GTTTTGTTTG 551 GCGACGGTGT TGGTGGCGAT GCAGCGGTCG AGTGCCGAGG AAAGTGCTTG 601 TGCAAATGCG TTCATTGCGG GAATACGTT . GGGGGAAAAC TTGCGGATTT 651 TACCACGATT CTTGCGTTGT CGGCAGACGG CGGCGGTTTG GTGGTACAAT 701 GTGCGCCGTT TGCAGCCTTA AGGTGTTTCT GTATTTTTGG AGTATGGAAA 751 CGCATTCGGG CTGTTTTTTG CGGAAGACGG TAA This corresponds to the amino acid sequence <SEQ ID 2248; ORF 681.a>: a681.pep 1 ITTPMAISAS NFSEEAKFIS AMGISSAPGT VATVMFSSAT PNSWRVRQQT 51 LSISLPISLV KRACTMPMRR CLPSRLGAMV FIECPLVFGG FGMPSEGSVL 101 RLPVGDGLEC AVFCQFPRAA FRLGEQCGGF RVGFGDIGEA DDAEWRWG 151 VFVGLVAAEE TPAAWFKNG GFAVEEADGL VLFGDGVGGD AAVECRGKCL 201 CKCVHCGNTX GGKLADFTTI LALSADGGGL WQCAPFAAL RCFCIFGVWK 251 RIRAVFCGRR * m681/a681 90.8% identity in 260 aa overlap 10 20 30 40 50 60 m681 •Pep MTTPMAISASNFSEEAKFISAMGISSAPGTVATVMFSSATPNSWRVRQQTLSISLPISLV : II I II II I I I II I II II II II I II II II I II I II II II II II II II I II II II II II I I a681 ITTPMAISASNFSEEAKFISAMGISSAPGTVATVMFSSATPNSWRVRQQTLSISLPISLV 10 20 30 40 50 60 70 80 90 100 110 120 m681.pep KRACTMPMRRCLPSRLGAMVFIGCPLVFGVSECRLKVSVLRLPVGDGLECAVFGKLPCAA I I II II I I II II II II I II I II MMM : I II II II II II II II I :: I II a681 KRACTMPMRRCLPSRLGAMVFIECPLVFGGFGMPSEGSVLRLPVGDGLECAVFCQFPRAA 70 80 90 100 110 120 130 140 150 160 170 180 m681. pep FGLGEQCGGFRVGFGDVGEADDAEWRIVGVFVGLVAAEETPAAWFKNGGFAVEEADGP I II II I II II II II I : II II II II II : I II II I I II II I II II II II II II II II II I a681 FRLGEQCGGFRVGFGDIGEADDAEWRWGVFVGLVAAEETPAAWFKNGGFAVEEADGL 130 140 150 160 170 180 190 200 210 220 230 240 m681.pep VLFGDGVGGDTAVECRGKCLCKCVHYGNTLGXKLTDFTTIRALSADGGGLWQCAPFAAL I II I II II I M II II II II II I II I III I I I : II I II II I II II II II I II II II I a681 VLFGDGVGGDAAVECRGKCLCKCVHCGNTXGGKLADFTTILALSADGGGLWQCAPFAAL 190 200 210 220 230 240 250 260 m681.pep RCFCIFGVWKRIRAVFCGRRX II II II II II II II I II II M a681 RCFCIFGVWKRIRAVFCGRRX 250 260 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2249>: g682. seq 1 ATGCGCGATT TCGCCGTATG GGTGCCTTAC GGGGAACGGC GGAAAAATTG 51 GGACATAAGG TATTGCCTCC CGCACCTTAT TCGCCTGAGC CCAACCCGAT 101 TGAGAAAGTG TGGGCGAATA TTAAGCGGTA TCTGCGAACC GTTTTGTCTG 151 ATTACGCCCG ATTTGACGAT GCACTACTGT CCTATTTTGA TTTTAATTGA 201 CTATATTTGT GTGAATGATG AAATAAAAAT GCCGTCTGAA CCCGATTGGA 251 TTCAGACGGC ATTTTGTATG GCAGGATTTA TTCGCTTTCC AACTGACCGA 301 CCCATTCTGA CAAGGCAGTC AGGCGTTGTT CGGATTTCGC CACGAACGGG 351 TTTTCGGTAT CCCACGCGTA GCCTGCCAAA ATCGAAGAAA GCATACGGCT 401 GA This corresponds to the amino acid sequence <SEQ ID 2250; ORF 682>: 1 MRDFAVWVPY GERRKΝWDIR YCLPHLIRLS PTRLRKCGRI LSGICEPFCL 51 ITPDLTMHYC PILILIDYIC VΝDEIKMPSE PDWIQTAFCM AGFIRFPTDR 101 PILTRQSGW RISPRTGFRY PTRSLPKSKK AYG* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2251>: m682. seq 1 ATGCGTGATT TCACCGTATG GGTGTCTTAC GGGAAATGGC GGAAAAATTG 51 GGACATAAGG TATTGCCTCT TGCACCTTAT TCACCTGAGC TCAACCCGAT 101 TGAGAAAGTG TGGGCGAATA TTAAGCGGTA TCTGCGAACC GTTTTGTCTG 151 ATTACGCCCG ATTTGACGAT GCACTACTGT CCTATTTTGA TTTTAATTGA 201 CTAT GAAA TGGCAATGCC GTCTGAACCC GATTGGATTC 251 AGACGGCATT TTGTATGGCG TACGGATTTA TTCGGTTTCC AACTGACCGA 301 CCCATTCGGA CAAGGCAGTC AGGCGTTGTT CGGATTTCGC CACGAACGGG 351 TTTTCGGTAT CCCACGCGTA GCCTGCCAAA ATCGAAGAAA GCATACGGCT 401 GA This corresponds to the amino acid sequence <SEQ ID 2252; ORF 682>: m68 .pep 1 MRDFTVWVSY GKWRKΝWDIR YCLLHLIHLS STRLRKCGRI LSGICEPFCL 51 ITPDLTMHYC PILILIDY.. ..EMAMPSEP DWIQTAFCMA YGFIRFPTDR 101 PIRTRQSGVV RISPRTGFRY PTRSLPKSKK AYG* Computer analysis of the amino acid sequences gave the following results: Homology with a predicted ORF from N meningitidis menA with menB ORF 682 shows 88.1% identity over a 134 aa overlap with a predicted ORF (ORF682.a) from N gonorrhoeae: m682/g682 10 20 30 40 50 60 m682 . pep MRDFTVWVSYGKWRKNWDIRYCLLHLIHLSSTRLRKCGRILSGICEPFCLITPDLTMHYC I I I I : I I I I I : I I I I I I I I I I M M M M M M M M M M M M M M I M M M g682 MRDFAVWVPYGERRKNWDIRYCLPHLIRLSPTRLRKCGRILSGICEPFCLITPDLTMHYC 10 20 30 40 50 60 70 80 90 100 110 m682. pep PILILIDY EMAMPSEPDWIQTAFCMAYGFIRFPTDRPIRTRQSGWRISPRTGFR I II II II I I : II II I II II II II II II II II II I II II II I I II I II II II I g682 PILILIDYICVNDEIKMPSEPDWIQTAFCMA-GFIRFPTDRPILTRQSGWRISPRTGFR 70 80 90 100 110 120 130 m682.pep YPTRSLPKSKKAYGX MMMMIMMM g682 YPTRSLPKSKKAYGX 120 130 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2253>: a682.seq 1 ATGCGCGATT TTACCGTATG GGTGTCTTAC GGGAAATGGC GGAAAAATTG 51 GGACATAAGG TATTGCCTCT TGCACCTTAT TCACCTGAGC TCAACCCGAT 101 TGAGAAAGTG TGGGCGAATA TTAAGCGGTA TCTGCGAACC GTTTTGTCTG 151 ATTACGCCCG ATTTGACGAT GCACTACTGT CCTATTTTGA TTTTAATTGA 201 ATAT 251 TATA TTCGGTTTCC AACTGACCGA 301 CCCATTCTGA CAAGGCCGAC AGGCGTTGTT CGGATTTCGC CACGAACGGG 351 TTTTCGGTAT CCCACGCGTA GCCTGCCAAA ATCGAAGAAA GCATACGGCT 401 GA This corresponds to the amino acid sequence <SEQ ID 2254; ORF 682.a>: a682.pep 1 MRDFTVWVSY GKWRKΝWDIR YCLLHLIHLS STRLRKCGRI LSGICEPFCL 51 ITPDLTMHYC PILILIEY YIRFPTDR 101 PILTRPTGW RISPRTGFRY PTRSLPKSKK AYG* m682/a682 80.6% identity in 129 aa overlap 10 20 30 40 50 60 m682.pep MRDFTVWVSYGKWRKΝWDIRYCLLHLIHLSSTRLRKCGRILSGICEPFCLITPDLTMHYC II II II II I II II II II II II II II I II I II II II II I II I I II II II II I II I II II II a682 MRDFTVWVSYGKWRKΝWDIRYCLLHLIHLSSTRLRKCGRILSGICEPFCLITPDLTMHYC 10 20 30 40 50 60 70 80 90 100 110 120 m682.pep PILILIDYEMAMPSEPDWIQTAFCMAYGFIRFPTDRPIRTRQSGWRISPRTGFRYPTRS I II II M I : II II II II I II : I II I II II I II II II II a682 PILILIEY YIRFPTDRPILTRPTGWRISPRTGFRYPTRS 70 80 90 100 130 m682.pep LPKSKKAYGX II II II II II a682 LPKSKKAYGX 110 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 2255> 1 ATGATTAAGG AAACCCTAAT GCGCCCAATC TTCCTATCTT TCGTTTTACT 51 CCCTATTTTG ATAACCGCCT GCAGCACACC GGACAAGTCT GCCCGATGGG 101 AAAATATCGG CACAATCTCA AACGGCAATA TTCATACATA TATTAATAAA 151 GACAGTGTGA GAAAAAACGG AAATCTGATG ATTTTCCAAG ATAAAAAAGT 201 TGTTACCAAT CTGAAACAAG AACGTTTTGC CAACACCCCC GCATACAAGA 251 CTGCCATTGC CGAGTGGGAA ATCCACTGCA ACAACAAAAC ATACCGCTTA 301 AGTTCGCTAC AGTTATTTGA TACAAAAAAC ACGGAAATTT CCACACAAAA 351 CTACACAGCC TCTTCCCTCC GCCCGATGAG CATCCTGTCC GGGACATTAA 401 CTGAAAAACA ATATGAAACC GTATGCGGGA AAAAACTCTG A This corresponds to the amino acid sequence <SEQ ID 2256; ORF 683 >: g683.pep 1 MIKETLMRPI FLSFVLLPIL ITACΞTPDKS ARWENIGTIS NGNIHTYINK 51 DSVRKNGNLM IFQDKKVVTN LKQERFANTP AYKTAIAEWE IHCNNKTYRL 101 SSLQLFDTKN TEISTQNYTA SSLRPMSILS GTLTEKQYET VCGKKL* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2257>: m683.seq.. 1 ATGATTAAGG AAACCCTAAT GCGCCCAATC TTCCTATCTT TCGTTTTATT 51 CCCTATTTTG ATAACCGCCT GCAGCACACC GGACAAGTCT GCCCGATGGG 101 AAAATATCGG CACAATCTCA AACGGCAATA TTCATACATA TATCAATAAA 151 GACAGCGTGA GAAAAAACGG AAATCTGATG ATTTTCCAAG ATAAAAAAGT 201 TGTTACCAAT CTAAAACAAG AACGTTTTGC CAACACCCCC GCATACAAGA 251 CTGCCATTGC CGAGTGGGAA ATCCACTGCA ACAACAAAAC ATACCGCTTA 301 AGTTCGCTAC AGTTGTTTGA TACAAAAAAC ACGGAAATTT CCACACAAAA 351 CTACACAGCC TCTTCCCTCC GCCCGATGAG CATCCTGTCC GGGACATTAA 01 CCGAAAAACA ATATGAAACC GTATGCGGAA AAAAACTCTG A This corresponds to the amino acid sequence <SEQ ID 2258; ORF 683>: m683.pep.. 1 MIKETLMRPI FLSFVLFPIL ITACΞTPDKS ARWENIGTIS NGNIHTYINK 51 DSVRKNGNLM IFQDKKVVTN LKQERFANTP AYKTAIAEWE IHCNNKTYRL 101 SSLQLFDTKN TEISTQNYTA SSLRPMSILS GTLTEKQYET VCGKKL* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N. gonorrhoeae ORF 683 shows 99.3% identity over a 146 aa overlap with a predicted ORF (ORF 683) from N gonorrhoeae: m683/g683 99.3% identity in 146 aa overlap 10 20 30 40 50 60 m683.pep MIKETLMRPIFLSFVLFPILITACSTPDKSARWEΝIGTISΝGΝIHTYIΝKDSVRKΝGΝLM I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g683 MIKETLMRPIFLSFVLLPILITACSTPDKSARWEΝIGTISΝGΝIHTYIΝKDΞVRKΝGΝLM 10 20 30 40 50 60 70 80 90 100 110 120 m683.pep IFQDKKVVTΝLKQERFAΝTPAYKTAIAEWEIHCΝΝKTYRLSSLQLFDTKΝTEISTQΝYTA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g683 IFQDKKVVTΝLKQERFAΝTPAYKTAIAEWEIHCΝΝKTYRLSΞLQLFDTKΝTEIΞTQΝYTA 70 80 90 100 110 120 130 140 m683.pep SSLRPMSILSGTLTEKQYETVCGKKLX I I I I I I I II I II I I I I I I I I I I II I I I g683 SSLRPMSILSGTLTEKQYETVCGKKLX 130 140 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2259> a683. seq 1 ATGATTAAGG AAACCCTAAT GCGCCCAATC TTCCTATCTT TCGTTTTATT 51 CCCTATTTTG ATAACCGCCT GCAGCACACC GGACAAGTCT GCCCGATGGG 101 AAAATATCGG CACAATCTCA AACGGCAATA TTCATACATA TATCAATAAA 151 GACAGCGTGA GAAAAAACGG AAATCTGATG ATTTTCCΝAG ATAAAAAAGT 201 TGTTACCAAT CTAAAACAAG AACGTTTTGC CΝACACCCCC GCATACAAGA 251 CTGCCATTGC CGAGTGGGAA ATCCACTGCA ACAACAAAAC ATACCGCTTA 301 AGTTCGCTAC AATTGTTTGA TACAAAAAAC ACGGAAATTT CCACACAAAA 351 ΝTACACAGCC TCTTCCCTCC GCCCGATGAG CATCCTGTCC GGGACATTAA 401 CCGAAAAACA ATATGAAACC GTATGCGGAA AAAAACTCTG A This corresponds to the amino acid sequence <SEQ ID 2260; ORF 683. a>: a683.pep 1 MIKETLMRPI FLSFVLFPIL ITACΞTPDKS ARWENIGTIS NGNIHTYINK 51 DSVRKNGNLM IFXDKKVVTN LKQERFAXTP AYKTAIAEWE IHCNNKTYRL 101 SSLQLFDTKN TEISTQXYTA SSLRPMSILS GTLTEKQYET VCGKKL* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N. meningitidis ORF 683 shows 97.9% identity over a 146 aa overlap with a predicted ORF (ORF 683) from N meningitidis: m683/a683 97.9% identity in 146 aa overlap 10 20 30 40 50 60 m683.pep MIKETLMRPIFLΞFVLFPILITACSTPDKSARWEΝIGTISΝGΝIHTYIΝKDSVRKΝGΝLM I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a683 MIKETLMRPIFLSFVLFPILITACΞTPDKSARWEΝIGTISΝGΝIHTYIΝKDΞVRKΝGΝLM 10 20 30 40 50 60 70 80 90 100 110 120 m683.pep IFQDKKVVTΝLKQERFAΝTPAYKTAIAEWEIHCΝΝKTYRLΞSLQLFDTKΝTEIΞTQΝYTA II II I I I I I I I II I II I I I I I I I I II I I I I I I I I I I I I I I III a683 IFXDKKVVTΝLKQERFAXTPAYKTAIAEWEIHCΝΝKTYRLSSLQLFDTKΝTEISTQXYTA 70 80 90 100 110 120 130 140 m683.pep SSLRPMSILSGTLTEKQYETVCGKKLX I I I I I I I I I I I I I I I I I I I I I I I I I I I a683 SSLRPMSILSGTLTEKQYETVCGKKLX 130 140 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 2261> 1 ATGCGCCTTT TCCCCATCGC CGCCGCCCTG ACGCTTGCCG CCTGCGGTAC 51 TGTGCAAAGC ACACAATATT TCGTGTTGCC CGACAGCCGC TACATCCGTC 101 CTGCAACGCA AGGCGGCGAA ACCGCCGTCG AAGTCCGTCT TGCCGAACCG 151 CTCAAACGCG GCGGACTGGT CTATCAAACC GACCCCTACC GCATCAACAC 201 CGCACAAAAC CATGTTTGGG CAGACACCTT GGACGATATG CTCGAAGCGG 251 CGTTGAGCAA TGCATTCAAC CGTTTGGACA GCACACGCAC CTTTGTTCCT 301 GCCTCACGCA GCGGCAGTAC CGACAAATGG ACGGTCTATA TCGACGCATT 351 CCAAGGCAGC TACACGGGCA AAACCCTCAT CAGCGGCTAC GCCGTCCTAC 401 CCGACGGTAC GAACAGACCC TTCCATATCG AAACCGAACA GCAGGGTGAC 451 GGCTACGCCG CCATGACCGC CGCACTCGAA CAGGGACTGA AACAGGCGGC 501 GCAACAGATG GTCGAGTAA This corresponds to the amino acid sequence <SEQ ID 2262; ORF 684 >: g684.pep 1 MRLFPIAAAL TLAACGTVQS TQYFVLPDSR YIRPATQGGE TAVEVRLAEP 51 LKRGGLVYQT DPYRIΝTAQΝ HVWADTLDDM LEAALSΝAFΝ RLDΞTRTFVP 101 ASRSGSTDKW TVYIDAFQGS YTGKTLISGY AVLPDGTΝRP FHIETEQQGD 151 GYAAMTAALE QGLKQAAQQM VE* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2263>: 684.seq 1 ATGCGCCTTT TCCCGATTGC CGCCGCCCTG TCGCTTGCCG CCTGCGGTAC 51 TGTGCAAAGC ACACAATATT TCGTGTTGCC CGACAGCCGC TACATCCGTC 101 CTGCAACGCA AGGCGGCGAA ACTGCCGTCG AAGTCCGTCT TGCCGAACCG 151 CTCAAACGCG GCGGACTGGT CTATCAAACC GACCCCTACC GCCTCAACAC 201 CGCACAAAAC CACGTCTGGG CAGACACCTT GGACGATATG CTCGAAGCGG 251 CGTTGAGCAA TGCATTCAAC CGTTTGGACA GCACACGCAT CTTTGTTCCT 301 GCCTCACGCA GCGGCAGTAC CGAAAAATGG ACGGTCTATA TCGACGCATT 351 CCAAGGCAGC TACACGGGCA AAACCCTCAT CAGCGGCTAC GCCGTCCTAC 401 CCGACGGTAC GAACAGACCC TTCCATATCG AAACCGAACA GCAGGGTGAC 451 GGCTACGCCG CGATGACCGC CGCACTCGAA CAGGGACTGA AACAGGCGGC 501 GCAACAGATG GTCGAGTAA This corresponds to the amino acid sequence <SEQ ID 2264; ORF 684>: m684.pep 1 MRLFPIAAAL SLAACGTVQS TQYFVLPDSR YIRPATQGGE TAVEVRLAEP 51 LKRGGLVYQT DPYRLΝTAQΝ HVWADTLDDM LEAALSΝAFΝ RLDΞTRIFVP 101 ASRSGSTEKW TVYIDAFQGS YTGKTLISGY AVLPDGTΝRP FHIETEQQGD 151 GYAAMTAALE QGLKQAAQQM VE* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 684 shows 97.7% identity over a 172 aa overlap with a predicted ORF (ORF 684) from N gonorrhoeae: m684/g684 97.7% identity in 172 aa overlap 10 20 30 40 50 60 m68 .pep MRLFPIAAALΞLAACGTVQSTQYFVLPDSRYIRPATQGGETAVEVRLAEPLKRGGLVYQT I I I I I I I I I l II I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I M I I g684 MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVEVRLAEPLKRGGLVYQT 10 20 30 40 50 60 70 80 90 100 110 120 m684.pep DPYRLΝTAQΝHVWADTLDDMLEAALΞΝAFΝRLDΞTRIFVPAΞRΞGSTEKWTVYIDAFQGS I I I IM I I I I I I I I I I I I I I I I I I I I I I I II II I I I I I I I I I I I I l I I I I I I I I I I I g684 DPYRIΝTAQΝHVWADTLDDMLEAALSΝAFΝRLDSTRTFVPASRSGSTDKWTVYIDAFQGS 70 80 90 100 110 120 130 140 150 160 170 m684.pep YTGKTLIΞGYAVLPDGTΝRPFHIETEQQGDGYAAMTAALEQGLKQAAQQMVEX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g684 YTGKTLISGYAVLPDGTΝRPFHIETEQQGDGYAAMTAALEQGLKQAAQQMVEX 130 140 150 160 170 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2265> a684.seq 1 ATGCGCCTCT TCCCGATTGC CGCCGCCCTG ACGCTTGCCG CCTGCGGTAC 51 TGTGCAAAGC ACACAATATT TCGTGTTGCC CGACAGCCGC TACATCCGTC 101 CTGCAACGCA AGGCGGCGAA ACTGCCGTCG AAGTCCGTCT TGCCGAACCG 151 CTCAAACGCG GCGGACTGGT CTATCAAACC GACCCCTACC GCCTCAACAC 201 CGCACAAAAC CACGTCTGGG CAGACACCTT GGACGATATG CTCGAAGCGG 251 CGTTGAGCAA TGCATTCAAC CGTTTGGACA GCACACGCAT CTTTGTTCCT 301 GCCTCACGCA GCGGCAGTAC CGAAAAATGG ACGGTCTATA TCGACGCATT 351 CCAAGGCAGC TACACGGGCA AAACCCTCAT CAGCGGCTAC GCCGTCCTAC 401 CCGACGGTAC GAACAGACCC TTCCATATCG AAACCGAACA GCAGGGTGAC 451 GGCTACGCCG CCATGACCGC CGCACTCGAA CAGGGACTGA AACAGGCGGC 501 GCAACAGATG GTCGAGTAA This corresponds to the amino acid sequence <SEQ ID 2266; ORF 684.a>: a68 .pep 1 MRLFPIAAAL TLAACGTVQS TQYFVLPDSR YIRPATQGGE TAVEVRLAEP 51 LKRGGLVYQT DPYRLΝTAQΝ HVWADTLDDM LEAALSΝAFΝ RLDSTRIFVP 101 ASRSGSTEKW TVYIDAFQGS YTGKTLISGY AVLPDGTΝRP FHIETEQQGD 151 GYAAMTAALE QGLKQAAQQM VE* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 684 shows 99.4% identity over a 172 aa overlap with a predicted ORF (ORF 684) from N meningitidis m684/a684 99.4% identity in 172 aa overlap 10 20 30 40 50 60 m684.pep MRLFPIAAALSLAACGTVQSTQYFVLPDSRYIRPATQGGETAVEVRLAEPLKRGGLVYQT II II I I I II l I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a684 MRLFPIAAALTLAACGTVQSTQYFVLPDΞRYIRPATQGGETAVEVRLAEPLKRGGLVYQT 10 20 30 40 50 60 70 80 90 100 110 120 m684.pep DPYRLΝTAQΝHVWADTLDDMLEAALSΝAFΝRLDSTRIFVPASRSGSTEKWTVYIDAFQGS I I I II II I I I II I I I I I I I I II I I I I I II II I I I I I I I I I I I I I I II II I I I I I I I || I I a684 DPYRLΝTAQΝHVWADTLDDMLEAALSΝAFΝRLDSTRIFVPASRSGSTEKWTVYIDAFQGS 70 80 90 100 110 120 130 140 150 160 170 m684 . pep YTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALEQGLKQAAQQMVEX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I a684 YTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALEQGLKQAAQQMVEX 130 140 150 160 170 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2267> g685.seq 1 TTGTTTTGCC GTATCGGGAA TTTTGCGTTT TGCGGCGTGG TTTCTGCAGG 51 TTGTTTGCTT AATAATAAAC ATTCTTATTC GTATGCAAAG GAACCGCACA 101 CCGTGAAACC GCGTTTTTAT TGGGCAGcct GCGCCGTCCT GCCGGCCGCC 151 TGTTCGCCCG AACCTGCCGC CGAAAAAACT GTATccgCCG CATCCCAAGC 201 CGCATCCACA CCTGTCGCCA CGCTGACCGT GCCGACCGCG CGGGGCGATG 251 CCGTTGTGCC GAAGAATCCC GAACgcgtcg ccgtgtAcga CtggGCGGCG 301 TtggaTACGC TGACCGAGCC GGGCGTGAAT GTGGGCGCAA CCACCGCGCC 351 GGTGCGCGTG GACTATTTGC AGCCTGCATT TGACAAGGCG GCAACGGTGG 401 GGACGCTGTT TGAGCCCGAT TGCGAATCCC TGCACCGCCA CAATCCGCAG 451 TTTGTCATTA CCGGCGGGCC GGGTGCGGAA GCGTATGAAC AGTTGGCGAA 501 AAACGCGACC ACCATAGATT TGACGGTGGA CAACGGCAAT ATCCGCACCA 551 GCGGCGAGAA GCAGATGGAG ACCCTGTCGC GGATTTTCGG TAAGGAAGCG 601 CGCGTGGCGG AATTGAATGC GCAGATTGAC GCGCTGTTCG CCCAAAAGCG 651 CGAAGCCGCC AAAGGCAAAG GACGCGGGCT GGTGCTGTCG GTTACAGGCA 701 ACAAGGTGTC CGCCTTCGGC ACGCAATCGC GGTTGGCAAG TTGGATACAC 751 GGCGACATCG GCCTGCCGCC CGTGGACGAA TCTTTACGCA ACGAAGGGCA 801 CGGGCAGCCC GTTTCCTTCG AATACATCAA AGAGAAAAAC CCCGGCTGGA 851 TTTTCATCAT CGACCGCACC GCCGCCATCG GGCAGGAAGG GCCGGCTGCC 901 GTGGAAGTGT TGGATAACGC GCTGGTATGC GGCACGAACG CTTGGAAGCG 951 CAAGCAAATC ATCGTCATGC CTGCCGCGAA CTACATTGTC GCGGGCGGCG 1001 CGCGGCAGTT GATACAGGCG GCGGAACAGT TGAAGGCGGC GTTTGAAAAG 1051 GCAGAACCCG TTGCGGCGCA GTAG This corresponds to the amino acid sequence <SEQ ID 2268; ORF 685 >: g685.pep 1 LFCRIGΝFAF CGVVSAGCLL ΝΝKHSYSYAK EPHTVKPRFY WAACAVLPAA 51 CSPEPAAEKT VSAAΞQAAST PVATLTVPTA RGDAVVPKΝP ERVAVYDWAA 101 LDTLTEPGVΝ VGATTAPVRV DYLQPAFDKA ATVGTLFEPD CEΞLHRHΝPQ 151 FVITGGPGAE AYEQLAKΝAT TIDLTVDΝGΝ IRTSGEKQME TLΞRIFGKEA 201 RVAELΝAQID ALFAQKREAA KGKGRGLVLS VTGΝKVΞAFG TQSRLASWIH 251 GDIGLPPVDE ΞLRΝEGHGQP VΞFEYIKEKΝ PGWIFIIDRT AAIGQEGPAA 301 VEVLDΝALVC GTΝAWKRKQI IVMPAAΝYIV AGGARQLIQA AEQLKAAFEK 351 AEPVAAQ* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2269>: 685. seq 1 TTGTTTTGCC GTATCGGGAA TTTTGCGTTT TGCGGCGTGG TTTCTGCAGG 51 TTGTTTGCTT AATAATAAAC ATTCTTATTC GTATGCAAAG GAACCGCACA 101 CCGTGAAACC GCGTTTTTAT TGGGCAGCCT GCGCCGTCCT GCTGACCGCC 151 TGTTCGCCCG AACCTGCCGC CGAAAAAACT GTATCCGCCG CATCCGCATC 201 TGCCGCCACG CTGACCGTGC CGACCGCGCG GGGCGATGCC GTTGTGCCGA 251 AGAATCCCGA ACGCGTCGCC GTGTACGACT GGGCGGCGTT GGATACGCTG 301 ACCGAATTGG GCGTGAATGT GGGCGCAACC ACCGCGCCGG TGCGCGTGGA 351 TTATTTGCAG CCTGCATTTG ACAAGGCGGC AACGGTGGGG ACGCTGTTCG 401 AGCCCGATTA CGAAGCCCTG CACCGCTACA ATCCTCAGCT TGTCATTACC 451 GGCGGGCCGG GCGCGGAAGC GTATGAACAG TTAGCGAAAA ACGCGACCAC 501 CATAGATCTG ACGGTGGACA ACGGCAATAT CCGCACCAGC GGCGAAAAGC 551 AGATGGAGAC CTTGGCGCGG ATTTTCGGCA AGGAAGCGCG CGCGGCGGAA 601 TTGAAGGCGC AGATTGACGC GCTGTTCGCC CAAACGCGCG AAGCCGCCAA 651 AGGCAAAGGA CGCGGGCTGG TGCTGTCGGT TACGGGCAAC AAGGTGTCCG 701 CCTTCGGCAC GCAGTCGCGG TTGGCAAGTT GGATACACGG CGACATCGGC 751 CTACCGCCTG TAGACGAATC TTTACGCAAC GAGGGGCACG GGCAGCCTGT 801 TTCCTTCGAA TACATCAAAG AGAAAAACCC CGATTGGATT TTCATCATCG 851 ACCGTACCGC CGCCATCGGG CAGGAAGGGC CGGCGGCTGT CGAAGTATTG 901 GATAACGCGC TGGTACGCGG CACGAACGCT TGGAAGCGCA AGCAAATCAT 951 CGTCATGCCT GCCGCGAACT ACATTGTCGC GGGCGGCGCG CGGCAGTTGA 1001 TTCAGGCGGC GGAGCAGTTG AAGGCGGCGT TTAAAAAGGC AGAACCCGTT 1051 GCGGCGGGGA AAAAGTAG This corresponds to the amino acid sequence <SEQ ID 2270; ORF 685>: m685.pep 1 LFCRIGNFAF CGVVΞAGCLL NNKHSYΞYAK EPHTVKPRFY WAACAVLLTA 51 CSPEPAAEKT VSAASASAAT LTVPTARGDA VVPKNPERVA VYDWAALDTL 101 TELGVNVGAT TAPVRVDYLQ PAFDKAATVG TLFEPDYEAL HRYNPQLVIT 151 GGPGAEAYEQ LAKNATTIDL TVDNGNIRTS GEKQMETLAR IFGKEARAAE 201 LKAQIDALFA QTREAAKGKG RGLVLSVTGN KVSAFGTQSR LAΞWIHGDIG 251 LPPVDEΞLRN EGHGQPVSFE YIKEKNPDWI FIIDRTAAIG QEGPAAVEVL 301 DNALVRGTNA WKRKQIIVMP AANYIVAGGA RQLIQAAEQL KAAFKKAEPV 351 AAGKK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 685 shows 94.4% identity over a 356 aa overlap with a predicted ORF (ORF 685) from N gonorrhoeae: m685/g685 94.4% identity in 356 aa overlap 10 20 30 40 50 60 m685.pep LFCRIGΝFAFCGVVΞAGCLLΝΝKHSYSYAKEPHTVKPRFYWAACAVLLTACSPEPAAEKT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I g685 LFCRIGΝFAFCGVVSAGCLLΝΝKHΞYSYAKEPHTVKPRFYWAACAVLPAACΞPEPAAEKT 10 20 30 40 50 60 70 80 90 100 110 m685. pep VSAASASA ATLTVPTARGDAVVPKΝPERVAVYDWAALDTLTELGVΝVGATTAPVRV I I I I I M I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g685 VSAASQAASTPVATLTVPTARGDAVVPKΝPERVAVYDWAALDTLTEPGVΝVGATTAPVRV 70 80 90 100 110 120 120 130 140 150 160 170 m685. pep DYLQPAFDKAATVGTLFEPDYEALHRYΝPQLVITGGPGAEAYEQLAKΝATTIDLTVDΝGΝ I I I I I I I I I I I I I I I I I I I I I: I I I : I I M I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g685 DYLQPAFDKAATVGTLFEPDCESLHRHΝPQFVITGGPGAEAYEQLAKΝATTIDLTVDΝGΝ 130 140 150 160 170 180 180 190 200 210 220 230 m685. pep IRTSGEKQMETLARIFGKEARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGΝKVSAFG I I I I I I I I I I I I : I I I I I I I Ml I l I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I g685 IRTSGEKQMETLSRIFGKEARVAELΝAQIDALFAQKREAAKGKGRGLVLΞVTGΝKVΞAFG 190 200 210 220 230 240 240 250 260 270 280 290 m685.pep TQSRLASWIHGDIGLPPVDESLRΝEGHGQPVSFEYIKEKΝPDWIFIIDRTAAIGQEGPAA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g685 TQSRLASWIHGDIGLPPVDEΞLRΝEGHGQPVSFEYIKEKΝPGWIFIIDRTAAIGQEGPAA 250 260 270 280 290 300 300 310 320 330 340 350 m685.pep VEVLDΝALVRGTΝAWKRKQIIVMPAAΝYIVAGGARQLIQAAEQLKAAFKKAEPVAAGKKX II I II I I I I II III I I I I II II I I I I I I I I I I II I II l II I I II g685 VEVLDΝALVCGTΝAWKRKQIIVMPAAΝYIVAGGARQLIQAAEQLKAAFEKAEPVAAQX 310 320 330 340 350 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2271> a685.seq 1 TTGTTTTGCC GTATCGGGAA TTTTGCGTTT TGCGGCGTGG TTTCTGCAGG 51 TTGTTTGCTT AATAATAAAC ATTCTTATTC GTATGCAAAG GAACCGCACA 101 CCGTGAAACC GCGTTTTTAT TGGGCAGCCT GCGCCGTCCT GCTGACCGCC 151 TGTTCGCCCG AACCTGCCGC CGAAAAAACT GTATCCGCCG CATCCGCATC 201 TGCCGCCACA CTGACCGTGC CGACCGCGCG GGGCGATGCC GTTGTGCCGA 251 AGAATCCCGA ACGCGTCGCC GTGTACGACT GGGCGGCGTT GGATACGCTG 301 ACCGAATTGG GTGTGAATGT GGGCGCAACC ACCGCGCCGG TGCGCGTGGA 351 TTATTTGCAG CCTGCATTTG ACAAGGCGGC AACGGTGGGG ACGCTGTTCG 401 AGCCCGATTA CGAAGCCCTG CACCGCTACA ATCCTCAGCT TGTCATTACC 451 GGCGGGCCGG GCGCGGAAGC GTATGAACAG TTGGCGAAAA ACGCGACCAC 501 CATAGATCTG ACGGTGGACA ACGGCAATAT CCGCACCAGC GGCGAAAAGC 551 AGATGGAGAC CTTGGCGCGG ATTTTCGGCA AGGAAGCGCG CGCGGCGGAA 601 TTGAAGGCGC AGATTGACGC GCTGTTCGCC CAAACGCGCG AAGCCGCCAA 651 AGGCAAAGGA CGCGGGCTGG TGCTGTCGGT TACGGGCAAC AAGGTGTCCG 701 CCTTCGGCAC GCAGTCGCGG TTGGCAAGTT GGATACACGG CGACATCGGC 751 CTACCGCCTG TAGACGAATC TTTACGCAAC GAGGGGCACG GGCAGCCTGT 801 TTCCTTCGAA TACATCAAAG AGAAAAACCC CGATTGGATT TTCATCATCG 851 ACCGTACCGC CGCCATCGGG CAGGAAGGGC CGGCGGCTGT CGAAGTATTG 901 GATAACGCGC TGGTACGCGG CACGAACGCT TGGAAGCGCA AGCAAATCAT 951 CGTCATGCCT GCCGCGAACT ACATTGTCGC GGGCGGCTCG CGGCAGTTGA 1001 TTCAGGCGGC GGAGCAGTTG AAGGAGGCGT TTGAAAAGGC AGAACCCGTT 1051 GCGGCGGGGA AAGAGTAG This corresponds to the amino acid sequence <SEQ ID 2272; ORF 685. a>: a685.pep 1 LFCRIGNFAF CGVVΞAGCLL NNKHSYΞYAK EPHTVKPRFY WAACAVLLTA 51 CSPEPAAEKT VSAASASAAT LTVPTARGDA VVPKNPERVA VYDWAALDTL 101 TELGVNVGAT TAPVRVDYLQ PAFDKAATVG TLFEPDYEAL HRYNPQLVIT 151 GGPGAEAYEQ LAKNATTIDL TVDNGNIRTΞ GEKQMETLAR IFGKEARAAE 201 LKAQIDALFA QTREAAKGKG RGLVLΞVTGN KVSAFGTQΞR LASWIHGDIG 251 LPPVDESLRN EGHGQPVSFE YIKEKNPDWI FIIDRTAAIG QEGPAAVEVL 301 DNALVRGTNA WKRKQIIVMP AANYIVAGGS RQLIQAAEQL KEAFEKAEPV 351 AAGKE* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 685 shows 98.9% identity over a 355 aa overlap with a predicted ORF (ORF 685) from N meningitidis: m685/a685 98.9% identity in 355 aa overlap 10 20 30 40 50 60 m685. pep LFCRIGΝFAFCGVVSAGCLLΝΝKHSYSYAKEPHTVKPRFYWAACAVLLTACSPEPAAEKT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a685 LFCRIGΝFAFCGVVΞAGCLLΝΝKHSYSYAKEPHTVKPRFYWAACAVLLTACΞPEPAAEKT 10 20 30 40 50 60 70 80 90 100 110 120 m685. pep VSAASASAATLTVPTARGDAVVPKΝPERVAVYDWAALDTLTELGVΝVGATTAPVRVDYLQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a685 VSAAΞASAATLTVPTARGDAVVPKΝPERVAVYDWAALDTLTELGVΝVGATTAPVRVDYLQ 70 80 90 100 110 120 130 140 150 160 170 180 m685.pep PAFDKAATVGTLFEPDYEALHRYΝPQLVITGGPGAEAYEQLAKΝATTIDLTVDΝGΝIRTS I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a685 PAFDKAATVGTLFEPDYEALHRYΝPQLVITGGPGAEAYEQLAKΝATTIDLTVDΝGΝIRTS 130 140 150 160 170 180 190 200 210 220 230 240 m685.pep GEKQMETLARIFGKEARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGΝKVSAFGTQSR I I I I I I I I I I I I I I I I I I I I II I I I I I II I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I a685 GEKQMETLARIFGKEARAAELKAQIDALFAQTREAAKGKGRGLVLΞVTGΝKVSAFGTQSR 190 200 210 220 230 240 250 260 270 280 290 300 m685.pep LASWIHGDIGLPPVDESLRΝEGHGQPVSFEYIKEKΝPDWIFIIDRTAAIGQEGPAAVEVL I I I II I III I II I III I III I I I I II I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I a685 LASWIHGDIGLPPVDESLRΝEGHGQPVSFEYIKEKΝPDWIFIIDRTAAIGQEGPAAVEVL 250 260 270 280 290 300 310 320 330 340 350 m685.pep DΝALVRGTΝAWKRKQIIVMPAAΝYIVAGGARQLIQAAEQLKAAFKKAEPVAAGKKX I llll I II II llll I I I I I I I I I I I I I I l I I II I II I I I MM I I I I I II l a685 DΝALVRGTΝAWKRKQIIVMPAAΝYIVAGGSRQLIQAAEQLKEAFEKAEPVAAGKEX 310 320 330 340 350 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 2273> g686.seq (partial) 1 ..AATTTCTCCT GCCGCGCCGA TGATGTTTTT GACGATATCT GCAGTGCCGT 51 TGAAGGCTTC ggcgGCATTG CCCGATCTGT CCAGCTCGGG GCTGTATCGG 101 GTGGCGCGTT TGAATCCGTC GCCTACTCCT TGCGTCAGCA TAGCGCCGGC 151 ATTGTGGAAA CGGTCGGCAA GCCGTTGTCC GGTGCTGCGG TTGTCGGTCA 201 GGTTGAGGCG GATATTTTGG GCAACGCCTT TTATGTCGTA GCTGTATATA 251 TCCCTCGCGC CTTTGGGAGC GGGATAGCCG CCGCCCTGTG GCCCGTCATA 301 GCCGTCGGCG GGATGGTGTT CGTATCCGTC CCAATGGATG CGGTAAAGGC 351 TGAATCCGTC AACGGGACTA CCGGCTTCGT CAGAATCGGA ATGTGA This corresponds to the amino acid sequence <SEQ ID 2274; ORF 686 >: g686.pep (partial) 1 ..NFSCRADDVF DDICSAVEGF GGIARSVQLG AVSGGAFESV AYSLRQHSAG 51 IVETVGKPLS GAAVVGQVEA DILGNAFYVV AVYIPRAFGS GIAAALWPVI 101 AVGGMVFVSV PMDAVKAESV NGTTGFVRIG M* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2275>: m686.seq.. 1 ATGATGTTGA AAAAATTCGT ACTCGGCGGT ATTGCCGCAT TGGTTTTGGC 51 GGCCTGCGGC GGTTCGGAAG GCGGCAGCGG AGCGΝΝΝΝΝΝ ΝΝΝΝΝΝAATT 101 TCTCCTGCAG CGCCGATGAT GTTTTTAACG ATATCTGCAG TGCCGTTGAA 151 GGCTTCGGCG GCATTGCCCG ATCTGTCCAG CTCGGGGCTG TATCGGGTGG 201 CGCGTTTGAA TCCGTCGCCT ACTCCTTGCG TCAGCATACT ACCGGCATTG 251 TGGAAACGGT CGGCAAGCCG TTGTCCGGTG CTGCGGTTGT CGGTCAGGTT 301 GAGGCGGATA TTTTGGGCAA CGCCTTTTAT GTCGTAGCTG TATATATCCC 351 TCGCGCCTTT GGGAGCGGGA TAGCCGCCGC CCTGTGGCCC GTCATAGCCG 401 TCGGCGGGAT GGTGTTCGTA TCCGTCCCAA TGGATGCGGT AAAGGCTAAA 451 TCCGTCAACG GGACTACCGG CTTCATCAGA ATCGGAATGT GA This corresponds to the amino acid sequence <SEQ ID 2276; ORF 686>: 686.pep 1 MMLKKFVLGG IAALVLAACG GSEGGSGAXX XXΝFSCΞADD VFΝDICSAVE 51 GFGGIARSVQ LGAVSGGAFE SVAYSLRQHT TGIVETVGKP LSGAAVVGQV 101 EADILGΝAFY VVAVYIPRAF GΞGIAAALWP VIAVGGMVFV SVPMDAVKAK 151 SVΝGTTGFIR IGM* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 686 shows 95.4% identity over a 131 aa overlap with a predicted ORF (ORF 686) from N gonorrhoeae g686/m686 95.4% identity in 131 aa overlap 10 20 30 g686.pep ΝFSCRADDVFDDICSAVEGFGGIARSVQLG I II I I I I I I : I I I I I I I I I I I I I I I I I I I m686 LKKFVLGGIAALVLAACGGSEGGSGAXXXXΝFSCSADDVFΝDICΞAVEGFGGIARSVQLG 10 20 30 40 50 60 40 50 60 70 80 90 g686.pep AVSGGAFESVAYSLRQHSAGIVETVGKPLSGAAVVGQVEADILGΝAFYVVAVYIPRAFGS I I I I I I I I I I I I I I I I M I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I m686 AVΞGGAFESVAYSLRQHTTGIVETVGKPLΞGAAVVGQVEADILGΝAFYVVAVYIPRAFGS 70 80 90 100 110 120 100 110 120 130 g686. pep GIAAALWPVIAVGGMVFVSVPMDAVKAESVΝGTTGFVRIGMX I I II I I I I I I I I I I I I I I II I I I I I I I : I I I I I I I I : I I I I I m686 GIAAALWPVIAVGGMVFVSVPMDAVKAKSVΝGTTGFIRIGMX 130 140 150 160 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2277> a686.seq (partial) 1 ..AATTTCTCCT GCCGCGCCGA TGATGTTTTT GACGATATCT GCAGTGCCGT 51 TGAAAGCTTC GGCGGCATTG CCCGATCTGT CCAGCTCGGG GCTGTATCGG 101 GTGGCGCGTT TGAATCCGTC GCCTACTCCT TGCGTCAGCft TACTACCGGT 151 ATTGTGGAAA CGGTCGACAA GCCGTTGTCC GGTGCTGCGG TTGTCGGTCA 201 GGTTGAGGCG GATATTTTGG GCAACGCCTT TTATGTCGTA GCTGTATATA 251 TCCCTCGCGC CTTTGGGAGC GGGATAGCCG CCGCCCTGTG GCCCGTCATA 301 GCCGTCGGCG GGATGGTGTT CGTATCCGTC CCAATGGATG CGGTAAAGGC 351 TGAATCCGTC AACGGGACTA CCGGCTTCAT CAGAATCGGA ATGTGA This corresponds to the amino acid sequence <SEQ ID 2278; ORF 686. a>: a686.pep (partial) 1 ..NFSCRADDVF DDICΞAVESF GGIARSVQLG AVSGGAFESV AYSLRQHTTG 51 IVETVDKPLS GAAVVGQVEA DILGNAFYVV AVYIPRAFGS GIAAALWPVI 101 AVGGMVFVSV PMDAVKAESV NGTTGFIRIG M* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 686 shows 96.2% identity over a 131 aa overlap with a predicted ORF (ORF 686) from N meningitidis: m686/a686 96.2% identity in 131 aa overlap 10 20 30 40 50 60 m686. pep LKKFVLGGIAALVLAACGGSEGGSGAXXXXΝFΞCSADDVFΝDICSAVEGFGGIARSVQLG llll I I I I Ml I I I I I I : I I I I I I I I I I I a686 ΝFSCRADDVFDDICSAVESFGGIARSVQLG 10 20 30 70 80 90 100 110 120 m686.pep AVSGGAFESVAYSLRQHTTGIVETVGKPLSGAAVVGQVEADILGΝAFYVVAVYIPRAFGS I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II a686 AVSGGAFESVAYSLRQHTTGIVETVDKPLΞGAAVVGQVEADILGΝAFYVVAVYIPRAFGS 40 50 60 70 80 90 130 140 150 160 m686.pep GIAAALWPVIAVGGMVFVSVPMDAVKAKSVΝGTTGFIRIGMX I I I I I I I I I I I I I I I I I I I I I I I I I I Ml I I I I I I I I I I I I I a686 GIAAALWPVIAVGGMVFVSVPMDAVKAESVΝGTTGFIRIGMX 100 110 120 130 The following partial DΝA sequence was identified in N. gonorrhoeae <SEQ ID 2279> g687.seq 1 ATGAAATCCA GACACCTCGC CCTCGCCCTC GGCGTTGCCG CCCTGTTCGC 51 CCTTGCCGCG TGCGACAGCA AAGTCCAAAC CAGCGTCCCC GCCGACAGCG 101 CGCCTGCCGC TTCGGCAGCC GCCGCCCCGG CAGGACTGGT CGAAGGGCAA 151 AACTACACCG TCCTTGCCAA CCCGATTCCC CAACAGCAGG CAGGCAAGGT 201 TGAAGTGCTT GAGTTTTTCG GCTATTTTTG TCCGCACTGC GCCCGCCTcg 251 AACCTGTTTT GAGCAAACAC GCCAAGTCTT TTAAAGACGA TATGTACCTG 301 CGTACCGAAC ACGTCGTCTG GCAGAAAGAA ATGCTGCCGC TGGCACGCct 351 CGCCGCCGCC GTCGATATGG CTGCCGCCGA AAGCAAAGAT GTGGCGAACA 401 GCCATATTTT CGATGCGATG GTCAACCAAA AAATCAAGCT GCAAGAGCCG 451 GAAGTCCTCA AAAAATGGCT GGGCGAACAa ACcgcctTTG ACGGCAAAAA 501 AGTCCTTGCC GCCTACGAAT CCCCCGAAAG TCAGGCGCGC GCcggcAAAA 551 TGCAGGAGCT GACCGAAACC TTCCAAATCG ACGGTACGCC CACGGTTATC 601 GTCGGCGGCA AATATAAAGT CGAATTTGCC GACTGGGAGT CCGGTATGAA 651 CACCATCGAC CTTTTGGCGG ACAAAGTACG TGAAGAACAA AAAGCCGCGC 701 AGTAG This corresponds to the amino acid sequence <2280 ID 724; ORF 687 >: g687.pep 1 MKSRHLALAL GVAALFALAA CDSKVQTSVP ADSAPAASAA AAPAGLVEGQ 51 ΝYTVLAΝPIP QQQAGKVEVL EFFGYFCPHC ARLEPVLSKH AKSFKDDMYL 101 RTEHVVWQKE MLPLARLAAA VDMAAAESKD VAΝSHIFDAM VΝQKIKLQEP 151 EVLKKWLGEQ TAFDGKKVLA AYESPESQAR AGKMQELTET FQIDGTPTVI 201 VGGKYKVEFA DWESGMΝTID LLADKVREEQ KAAQ* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2281>: 1 ATGAAATCCA GACACCTTGC CCTCgGCGTT GCCGCCCTGT TCGCCCTTGC 51 CGCGTGCGAC AGCAAAGTCC AAACCAGCGT CCCCGCCGAC AGCGCGCCTG 101 CCGCTTCGGC AGCCGCCGCC CCGGCAGGGC TGGTCGAAGG GCAAAACTAT 151 ACCGTCCTTG CCAACCCGAT TCCCCAACAG CAGGCAGGCA AAGTCGAAGT 201 CCTTGAGTTT TTCGGCTATT TCTGTCCGCA CTGCGCCCAC CTCGAACCTG 251 TTTTAAGCAA ACACGCCAAG TCTTTTAAAG ACGATATGTA CCTGCGTACC 301 GAACACGTCG TCTGGCAGAA AGAAATGCTG ACGCTGGCAC GCCTCGCCGC 351 CGCCGTCGAT ATGGCTGCCG CCGACAGCAA AGATGTGGCG AACAGCCATA 401 TTTTCGATGC GATGGTCAAC CAAAAAATCA AGCTGCAAAA TCCGGAAGTC 451 CTCAAAAAAT GGCTGGGCGA ACAAACCGCC TTTGACGGCA AAAAAGTCCT 501 TGCCGCCTAC GAGTCCCCCG AAAGCCAGGC GCGCGCCGAC AAAATGCAGG 551 AGCTGACCGA AACCTTCCAA ATCGACGGTA CGCCCACGGT TATCGTCGGC 601 GGTAAATATA AAGTTGAATT TGCCGACTGG GAGTCCGGTA TGAACACCAT 651 CGACCTTTTG GCGGACAAAG TACGCGAAGA ACAAAAAGCC GCGCAGTAA This corresponds to the amino acid sequence <SEQ ID 2282; ORF 687>: m687.ρep 1 MKΞRHLALGV AALFALAACD ΞKVQTΞVPAD SAPAASAAAA PAGLVEGQNY 51 TVLANPIPQQ QAGKVEVLEF FGYFCPHCAH LEPVLSKHAK ΞFKDDMYLRT 101 EHVVWQKEML TLARLAAAVD MAAADSKDVA NΞHIFDAMVN QKIKLQNPEV 151 LKKWLGEQTA FDGKKVLAAY ESPESQARAD KMQELTETFQ IDGTPTVIVG 201 GKYKVEFADW ESGMNTIDLL ADKVREEQKA AQ* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N. gonorrhoeae ORF 687shows 97.0% identity over a 234 aa overlap with a predicted ORF (ORF 687) from N gonorrhoeae: m687/g687 97.0% identity in 234 aa overlap 10 20 30 40 50 m687.pep MKΞRHLAL—GVAALFALAACDSKVQTSVPADSAPAAΞAAAAPAGLVEGQΝYTVLAΝPIP I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g687 MKSRHLALALGVAALFALAACDΞKVQTSVPADSAPAAΞAAAAPAGLVEGQΝYTVLAΝPIP 10 20 30 40 50 60 60 70 80 90 100 110 m687. pep QQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEHVVWQKEMLTLARLAAA I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g687 QQQAGKVEVLEFFGYFCPHCARLEPVLSKHAKSFKDDMYLRTEHVVWQKEMLPLARLAAA 70 80 90 100 110 120 120 130 140 150 160 170 m687.pep VDMAAADΞKDVAΝSHIFDAMVΝQKIKLQΝPEVLKKWLGEQTAFDGKKVLAAYESPESQAR I I I I I Ml I I I I I I I I I I I I I I I I I I I l I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g687 VDMAAAESKDVAΝSHIFDAMVΝQKIKLQEPEVLKKWLGEQTAFDGKKVLAAYESPESQAR 130 140 150 160 170 180 180 190 200 210 220 230 m687.pep ADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMΝTIDLLADKVREEQKAAQX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g687 AGKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMΝTIDLLADKVREEQKAAQX 190 200 210 220 230 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2283> a687. seq 1 ATGAAATCCA AACACCTCGC CCTCGGCGTT GCCGCCCTGT TCGCACTTGC 51 CGCGTGCGAC AGCAAAGTCC AAACCAGCGT CCCCGCCGAC AGCGCGCCTG 101 CCGCTTCGGC AGCCGCCGCC CCGGCAGGGC TGGTCGAAGG GCAAAACTAT 151 ACTGTCCTTG CCAACCCGAT TCCCCAACAG CAGGCAGGCA AAGTCGAAGT 201 CCTTGAGTTT TTCGGCTATT TCTGTCCGCA CTGCGCCCAC CTCGAACCTG 251 TTTTAAGCAA ACACGCCAAG TCTTTTAAAG ACGATATGTA CCTGCGTACC 301 GAACACGTCG TCTGGCAGAA AGAAATGCTG ACGCTCGCAC GCCTCGCCGC 351 CGCCGTCGAT ATGGCTGCCG CCGACAGCAA AGATGTGGCG AACAGCCATA 401 TTTTCGATGC GATGGTCAAC CAAAAAATCA AGCTGCAAGA GCCGGAAGTC 451 CTCAAAAAAT GGCTGGGCGA ACAAACCGCC TTTGACGGCA AAAAAGTCCT 501 TGCCGCTTAC GAATCTCCCG AAAGCCAGGC GCGCGCCGAC AAAATGCAGG 551 AGCTGACCGA AACCTTCCAA ATCGACGGTA CGCCCACGGT TATCGTCGGC 601 GGCAAATATA AAGTCGAATT TGCCGACTGG GAGTCCGGTA TGAACACCAT 651 CGACCTTTTG GCGGACAAAG TACGCGAAGA ACAAAAAGCC GCGCACTAA This corresponds to the amino acid sequence <SEQ ID 2284; ORF 687.a>: a687.pep 1 MKSKHLALGV AALFALAACD SKVQTΞVPAD SAPAASAAAA PAGLVEGQNY 51 TVLANPIPQQ QAGKVEVLEF FGYFCPHCAH LEPVLSKHAK SFKDDMYLRT 101 EHVVWQKEML TLARLAAAVD MAAADSKDVA NΞHIFDAMVN QKIKLQEPEV 151 LKKWLGEQTA FDGKKVLAAY ESPESQARAD KMQELTETFQ IDGTPTVIVG 201 GKYKVEFADW ESGMNTIDLL ADKVREEQKA AH* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 687 shows 98.7% identity over a 232 aa overlap with a predicted ORF (ORF 687) from N meningitidis: m687/a687 98.7% identity in 232 aa overlap 10 20 30 40 50 60 m687.pep MKSRHLALGVAALFALAACDSKVQTΞVPADSAPAASAAAAPAGLVEGQΝYTVLAΝPIPQQ I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a687 MKSKHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGLVEGQΝYTVLAΝPIPQQ 10 20 30 40 50 60 70 80 90 100 110 120 m687.pep QAGKVEVLEFFGYFCPHCAHLEPVLΞKHAKΞFKDDMYLRTEHVVWQKEMLTLARLAAAVD I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a687 QAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEHVVWQKEMLTLARLAAAVD 70 80 90 100 110 120 130 140 150 160 170 180 m687.pep MAAADΞKDVAΝSHIFDAMVΝQKIKLQΝPEVLKKWLGEQTAFDGKKVLAAYESPESQARAD I I I I I I I I I I I I I I I I I I I I I I I I I l I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a687 MAAADSKDVAΝΞHIFDAMVΝQKIKLQEPEVLKKWLGEQTAFDGKKVLAAYESPESQARAD 130 140 150 160 170 180 190 200 210 220 230 m687.pep KMQELTETFQIDGTPTVIVGGKYKVEFADWESGMΝTIDLLADKVREEQKAAQX I II I I I I I I I I I I I I I I I I I I I II I II I I I I I I I I I I I I I I I I II I I II I l a687 KMQELTETFQIDGTPTVIVGGKYKVEFADWE8GMΝTIDLLADKVREEQKAAHX 190 200 210 220 230 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2285> g688.seq 1 GTGCTACACT AGACATCCCG ATTTGCACAG AAAGGTTCTC CCGTGAACAA 51 AACCCTCATC CTCGCCCTTT CCGCCCTGTT CAGCCTGACC GCGTGCAGCG 101 TCGAACGCGT CTCGCTGTTT CCCTCCTACA AACTCAAAAT CATCCAAGGC 151 AACGAACTCG AACCGCGCGC CGTTGCCGCC CTGCGCCCCG GCATGACCAA 201 AGACCAAGTC CTGCTCCTGC TCGGCAGCCC CATACTGCGC GACGCTTTCC 251 ATACCGACCG CTGGGACTAT ACCTTCAACA CCTCCCGCAA CGGCATCATC 301 AAAGAACGCA GCAACCTGAC CGTCTATTTT GAAAACGGCG TACTCGTCCG 351 CACCGAAGGC GACGCCCTCC AAAATGCCGC CGAAGCCCTC CGCGCGAAAC 401 AAAACGCAGA CAAACAATAA This corresponds to the amino acid sequence <SEQ ID 2286; ORF 688 >: g688.pep 1 VLH*TSRFAQ KGSPVΝKTLI LALSALFSLT ACSVERVΞLF PSYKLKIIQG 51 ΝELEPRAVAA LRPGMTKDQV LLLLGSPILR DAFHTDRWDY TFΝTSRΝGII 101 KERSΝLTVYF EΝGVLVRTEG DALQΝAAEAL RAKQΝADKQ* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2287>: m688. seq 1 GTGTTACACT ACCCATCCCG ATTTGCACAG AAAGGCATTT CCGTGAACAA 51 AACCCTCATC CTCGCCCTTT CCGCCCTCCT CGGCCTTGCC GCGTGCAGTG 101 CCGAACGCGT TTCACTGTTC CCCTCGTACA AACTCAAAAT CATACAGGGC 151 AACGAACTCG AACCGCGCGC CGTTGCCGCC CTCCGCCCCG GCATGACCAA 201 AGACCAAGTC CTGCTCCTGC TCGGCAGCCC CATACTGCGC GACGCATTCC 251 ATACCGACCG CTGGGACTAT ACCTTCAACA CCTCCCGCAA CGGCATCATC 301 AAAGAACGCA GCAATCTGAC CGTCTATTTT GAAAACGGCG TACTCGTCCG 351 CACCGAAGGC GACGTCCTGC AAAACGCTGC CGAAGCCCTC AAAGACCGCC 401 AAAACACAGA CAAACCATAA This corresponds to the amino acid sequence <SEQ ID 2288; ORF 688>: 1 VLHYPSRFAQ KGISVΝKTLI LALΞALLGLA ACSAERVSLF PSYKLKIIQG 51 ΝELEPRAVAA LRPGMTKDQV LLLLGSPILR DAFHTDRWDY TFΝTSRΝGII 101 KERSΝLTVYF EΝGVLVRTEG DVLQΝAAEAL KDRQΝTDKP* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 688 shows 90.6% identity over a 138 aa overlap with a predicted ORF (ORF 688) from N gonorrhoeae: m688/g688 90.6% identity in 138 aa overlap 10 20 30 40 50 60 m688.pep VLHYPSRFAQKGISVΝKTLILALΞALLGLAACSAERVΞLFPSYKLKIIQGΝELEPRAVAA III I I I I I I I I I I I I :: Ml I Ml I I I I I I I I I I I I I I I I I I I I I I I I I g688 VLHXTSRFAQKGSPVΝKTLILALΞALFΞLTACSVERVSLFPΞYKLKIIQGΝELEPRAVAA 10 20 30 40 50 60 70 80 90 100 110 120 m688.pep LRPGMTKDQVLLLLGΞPILRDAFHTDRWDYTFΝTSRΝGIIKERSΝLTVYFEΝGVLVRTEG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g688 LRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFΝTSRΝGIIKERSΝLTVYFEΝGVLVRTEG 70 80 90 100 110 120 130 140 m688.pep DVLQΝAAEALKDRQΝTDKPX M I I I I I I I M M Ml I g688 DALQΝAAEALRAKQΝADKQX 130 140 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2289> a688.seq 1 GTGTTACACT ACCCATCCCG ATTTGCACAG AAAGGCATTT CCGTGAACAA 51 AACCCTCATC CTCGCCCTTT CCGCCCTCCT CGGCCTTGCC GCGTGCAGCG 101 TCGAACGCGT TTCACTGTTC CCCTCGTACA AACTCAAAAT CATACAGGGC 151 AACGAACTCG AACCTCGCGC CGTCGCCTCC CTCCGCCCCG GTATGACCAA 201 AGACCAAGTC CTGCTCCTGC TCGGCAGCCC CATACTGCGC GACGCATTCC 251 ATACCGACCG CTGGGACTAT ACCTTCAACA CCTCCCGCAA CGGCATCATC 301 AAAGACCGAA GCAATCTGAC CGTCTATTTT GAAAACGGCG TGCTCGTCCG 351 CACCGAAGGC AACGCCCTGC AAAATGCCGC CGAAGCCCTC CGCGTAAAAC 401 AAAACGCAGA CAAACAATAA This corresponds to the amino acid sequence <SEQ ID 2290; ORF 688. a>: a688.pep 1 VLHYPSRFAQ KGISVΝKTLI LALSALLGLA ACSVERVSLF PSYKLKIIQG 51 ΝELEPRAVAΞ LRPGMTKDQV LLLLGSPILR DAFHTDRWDY TFΝTSRΝGII 101 KDRSΝLTVYF EΝGVLVRTEG ΝALQΝAAEAL RVKQΝADKQ* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 688 shows 93.5% identity over a 138 aa overlap with a predicted ORF (ORF 688) from N meningitidis m688/a688 93.5% identity in 138 aa overlap 10 20 30 40 50 60 m688.pep VLHYPΞRFAQKGISVΝKTLILALSALLGLAACSAERVSLFPSYKLKIIQGΝELEPRAVAA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I : a688 VLHYPSRFAQKGIΞVΝKTLILALSALLGLAACSVERVSLFPSYKLKIIQGΝELEPRAVAS 10 20 30 40 50 60 70 80 90 100 110 120 m688.pep LRPGMTKDQVLLLLGΞPILRDAFHTDRWDYTFΝTSRΝGIIKERSΝLTVYFEΝGVLVRTEG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I a688 LRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFΝTSRΝGIIKDRSΝLTVYFEΝGVLVRTEG 70 80 90 100 110 120 130 140 m688.pep DVLQΝAAEALKDRQΝTDKPX ::| I I I I I I I : : I I : I I a688 ΝALQΝAAEALRVKQΝADKQX 130 140 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2291> g689. seq (partial) 1 .. TCTCCGCCCC TTCCTCCGAT GAGCGGAAAA CTGATGGCGG TTTTGATGGC 51 GGTACTGGTC GCGCTGATGC CGTTTTCCAT CGATGCCTAC CTGCCCGCGA 101 TTCCCGAAAT GGCGCAGCCG CTGAACGCGG ATATCCACCG TATCGAATAG 151 AGTCTGAGTT TGTTTATGTT CGGCACGGCG TTCGGGCAAG TGGCCGGCGG 201 CGCGGTGTCC GACATCAAAG GGCGCAAACC CGTCGCCCTG ACCGGTTTGA 251 TTGTATATTG CCTTGCCGTT GCCGCCATCG TATTTGCTTC GAGTACCGAA 301 CAGCTCCTTA ACCTGCGTGC GGTACAGGCG TTCGGCGCAG GCATGGCTGT 351 AGTCATCGTc ggtgcgatgg tgcgcgatTA TTATTCCGGA CGCAAAGCCG 401 cgcAGATGTT TGCCCTTATC GGCATCATTC TGATGGTTGT GCCGCTGGCC 451 GCACCCATGG TCGGCGCATT GTTGCAGGGA TTGGGCGGAT GGCGGGCGAT 501 TTTCGTTTTC ttggcGgcgT ATTCGCCGGT GCTGCCCGGT TTGGTACAGT 551 ATTTCCTGCC CAATCCCGCC GTCGGCGGCA AAATCGGCAG GGATGTGTTC 601 GGGCTGGTGG CGGGGCGGTT CAAGCGCGTA TTGAAAACCC GTGCCGCGAT 651 GGGTtatCTG TTTTTTCAGG CATTCAGCTT CGGTTCGATG TTCGCCTTTC 701 TGACCGAATC TTCCTTCGTG TACCGGCAGC TCTACCACGT TACGCCGCAC 751 CGGTACGCAT GGGTGTTTGC ACTCAACATC ATCACGATGA TGTTTTTCAG 801 CCGCGTTACC GCGTGGCGGC TTAAAACCGG CGCGCATCCG CAAAGCATCC 851 TGCTGCGGGG GATTGTCGTC CAATTTGCCG CCAACCCGTC CCAACTCGCC 901 GCCGTGCTGT TTTTCGGGTT GCCCCCGTTT TGGCTGCCGG TCGCGTGCGT 951 GATGTTTTCC GTCGGTACGC AGGGCCTGGT CGGTGCGGAC ACGCAGGCAT 1001 GCTTTATGTC TTATTTCAAA GAAGAGGGCG GCAGCGCGAA CGCCGTGTCG 1051 GGTGTATTCC GGTCCTTAAT CGGCGCGGGC GTGGTCATGG CGGCAACCGT 1101 GATGGCGGCA ACCATGACCG CGTCCGCCTC TTGCGGCATT GCGCTTTTGT 1151 GGCTCTGCTC GCACAAGGCG TGGAAGGAAA ACGAAAAAAA GCGAATACTT This corresponds to the amino acid sequence <SEQ ID 2292; ORF 689 >: g689.pep (partial) 1 ..SPPLPPMSGK LMAVLMAVLV ALMPFSIDAY LPAIPEMAQP LΝADIHRIE* 51 SLSLFMFGTA FGQVAGGAVΞ DIKGRKPVAL TGLIVYCLAV AAIVFASSTE 101 QLLΝLRAVQA FGAGMAVVIV GAMVRDYYSG RKAAQMFALI GIILMVVPLA 151 APMVGALLQG LGGWRAIFVF LAAYΞPVLPG LVQYFLPΝPA VGGKIGRDVF 201 GLVAGRFKRV LKTRAAMGYL FFQAFSFGSM FAFLTESΞFV YRQLYHVTPH 251 RYAWVFALΝI ITMMFFSRVT AWRLKTGAHP QSILLRGIVV QFAAΝPSQLA 301 AVLFFGLPPF WLPVACVMFΞ VGTQGLVGAD TQACFMSYFK EEGGSAΝAVS 351 GVFRSLIGAG VVMAATVMAA TMTASASCGI ALLWLCSHKA WKEΝEKKRIL The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2293>: m689.seq 1 TTGTTAATCC ACTATATCGT TCCGGTTCGT CCGGTTTTGC CGGGGCTTTT 51 GTTGCCGCCT GTTTGTGCCG GTGTGTTAAA ATTTTCCGTT TCCGCGTATT 101 GTGTTTTCCG CCGCCGGGCG GTTTGTTTGC GAATCGGACG AGAATTTATG 151 CCTTCTGCCC ATTATCCTGA AATGAGCGAA AAACTGATGG CGGTTTTGAT 201 GGCGATGCTG GTTACGCTGA TGCCGTTTTC CATCGATGCC TACCTGCCCG 251 CGATTCCCGA AATGGCGCAA TCGCTGAACG CGGATGTTCA CCGCATCGAA 301 CAGAGTTTGA GTTTGTTTAT GTTCGGCACG GCGTTCGGAC AGGTGGTCGG 351 CGGTTCGGTG TCCGACATCA AAGGGCGCAA ACCCGTCGCC CTGACCGGTT 401 TGATTGTATA TTGCCTTGCC GTTGCCGCCA TCGTATTTGT TTCGAGTGCC 451 GAACAGCTCC TCAACCTGCG CGTCGTGCAG GCATTCGGTG CGGGCATGAC 501 TGTGGTCATC GTCGGCGCAA TGGTGCGCGA TTATTATTCC GGACGCAAAG 551 CCGCCCAGAT GTTTGCCCTT ATCGGCATCA TTTTGATGGT TGTGCCGCTG 601 GTCGCACCCA TGGTCGGCGC ATTGTTGCAG GGCTTGGGTG GCTGGCAGGC 651 GATTTTTGTT TTTCTGGCGG CGTATTCGCT GGTGCTGCTC GGTTTGGTAC 701 AGTATTTCCT GCCCAAGCCC GCCGTCGGCG GCAAAATCGG ACGGGACGTG 751 TTCGGGCTGG TGGCGGGGCG GTTCAAGCGC GTATTGAAAA CCCGTGCTGC 801 GATGGGTTAT CTGTTTTTTC AGGCATTCAG CTTCGGTTCG ATGTTCGCCT 851 TTCTGACCGA ATCTTCCTTC GTGTACCAGC AGCTCTACCG TGTTACGCCT 901 CATCAATACG CTTGGGCGTT TGCACTCAAC ATCATCACGA TGATGTTTTT 951 CAACCGCGTT ACCGCGTGGC GGCTCAAAAC CGGCGTGCAT CCGCAAAGCA 1001 TCCTGCTGTG GGGGATTGTC GTCCAGTTTG CCGCCAACCT GTCCCAACTC 1051 GCCGCCGTGC TGTTTTTCGG GTTGCCCCCG TTTTGGCTGC TGGTCGCGTG 1101 CGTGATGTTT TCCGTCGGTA CGCAGGGCTT GGTCGGTGCA AACACGCAGG 1151 CGTGTTTTAT GTCCTATTTC AAAGAAGAGG GCGGCAGCGC AAACGCCGTA 1201 TTGGGTGTAT TCCAATCTTT AATCGGCGCG GGGGTGGGTA TGGCGGCGAC 1251 CTTCTTGCAC GACGGTTCGG CAACCGTGAT GGCGGCAACG ATGACCGCGT 1301 CCACCTCTTG CGGCATTGCG CTTCTGTGGC TCTGCTCGCA TCGTGCGTGG 1351 AAAGAAAACG GGCAAAGCGA ATACCTTTAA This corresponds to the amino acid sequence <SEQ ID 2294; ORF 689>: m689.pep 1 LLIHYIVPVR PVLPGLLLPP VCAGVLKFSV ΞAYCVFRRRA VCLRIGREFM 51 PSAHYPEMΞE KLMAVLMAML VTLMPFΞIDA YLPAIPEMAQ SLNADVHRIE 101 QSLSLFMFGT AFGQVVGGSV SDIKGRKPVA LTGLIVYCLA VAAIVFVΞSA 151 EQLLNLRVVQ AFGAGMTVVI VGAMVRDYYS GRKAAQMFAL IGIILMVVPL 201 VAPMVGALLQ GLGGWQAIFV FLAAYSLVLL GLVQYFLPKP AVGGKIGRDV 251 FGLVAGRFKR VLKTRAAMGY LFFQAFSFGS MFAFLTESΞF VYQQLYRVTP 301 HQYAWAFALN IITMMFFNRV TAWRLKTGVH PQSILLWGIV VQFAANLSQL 351 AAVLFFGLPP FWLLVACVMF SVGTQGLVGA NTQACFMΞYF KEEGGSANAV 401 LGVFQSLIGA GVGMAATFLH DGΞATVMAAT MTAΞTSCGIA LLWLCSHRAW 451 KENGQSEYL* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 689 shows 88.0% identity over a 408 aa overlap with a predicted ORF (ORF 689) from N. gonorrhoeae: m689/a689 88.0% identity in 408 aa overlap 30 40 50 60 70 80 m689.pep CAGVLKFSVSAYCVFRRRAVCLRIGREFMPSAHYPEMSEKLMAVLMAMLVTLMPFΞIDAY I I II I I I I I I I I : I l I I I I I I I I g689 SPPLPPMSGKLMAVLMAVLVALMPFSIDAY 10 20 30 90 100 110 120 130 140 m689.pep LPAIPEMAQSLΝADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLIVYCLAV lllllllll I I I Ml I I I I I I I I I I I I I I I I I : I M I I I I I I I I I I I I I I I I I I I I I I g689 LPAIPEMAQPLΝADIHRIEXSLSLFMFGTAFGQVAGGAVΞDIKGRKPVALTGLIVYCLAV 40 50 60 70 80 90 150 160 170 180 190 200 m689.pep AAIVFVSSAEQLLΝLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLV I I I I I : I I : I I I I I I M I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M g689 AAIVFASΞTEQLLΝLRAVQAFGAGMAVVIVGAMVRDYYΞGRKAAQMFALIGIILMVVPLA 100 110 120 130 140 150 210 220 230 240 250 260 m689.pep APMVGALLQGLGGWQAIFVFLAAYSLVLLGLVQYFLPKPAVGGKIGRDVFGLVAGRFKRV I I I I I I I I I I I I I M I I I I I II I I I II I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I g689 APMVGALLQGLGGWRAIFVFLAAYSPVLPGLVQYFLPΝPAVGGKIGRDVFGLVAGRFKRV 160 170 180 190 200 210 270 280 290 300 310 320 m689.pep LKTRAAMGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALΝIITMMFFΝRVT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I Ml I I : I I I I : I I M I I I I I I I I I I l I I g689 LKTRAAMGYLFFQAFSFGSMFAFLTEΞSFVYRQLYHVTPHRYAWVFALΝIITMMFFSRVT 220 230 240 250 260 270 330 340 350 360 370 380 m689.pep AWRLKTGVHPQΞILLWGIVVQFAAΝLΞQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGAΝ I I I I I I M I I I I I I I lllllllll I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : g689 AWRLKTGAHPQSILLRGIVVQFAAΝPSQLAAVLFFGLPPFWLPVACVMFSVGTQGLVGAD 280 290 300 310 320 330 390 400 410 420 430 440 m689.pep TQACFMSYFKEEGGSAΝAVLGVFQSLIGAGVGMAATFLHDGSATVMAATMTASTSCGIAL I I I I I I I I I I I I I I I I I I I I I Ml I I I I I I llll I I I I I I I I I : I I I I I I g689 TQACFMSYFKEEGGSAΝAVSGVFRSLIGAGVVMAAT VMAATMTASASCGIAL 340 350 360 370 380 450 460 m689.pep LWLCSHRAWKEΝGQSEYLX I I I I I I : I I I I I ::: I g689 LWLCSHKAWKEΝEKKRIL 390 400 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2295> a689.seq 1 TTGTTAATCC ACTATATCGT TCCGGTTCGT CCGGTTTTGC CGGGGCTTTT 51 GTTGCCGCCT GTTTGTGCCG GTGTGTTAAA ATTTTCCGTT TCCGCGTATT 101 GTGTTTTCCG CCGCCGGGCG GTTTGTTTGC GAATCGGACG AGAATTTATG 151 CCTTCTGCCC ATTATCCTGA AATGAGCGAA AAACTGATGG CGGTTTTGAT 201 GGCGATGCTG GTTACGCTGA TGCCGTTTTC CATCGATGCC TACCTGCCCG 251 CGATTCCCGA AATGGCGCAG TCGCTGAACG CGGATGTCCA CCGCATCGAA 301 CAGAGCCTGA GTTTGTTTAT GTTCGGCACG GCGTTCGGAC AGGTGGTCGG 351 CGGTTCGGTG TCCGACATCA AAGGGCGCAA ACCCGTCGCG CTGACCGGAC 401 TGGCCGTCTA CTGCCTTGCC GTTGCCGCCA TCGTATTTGC TTCGAGTGCC 451 GAACAGCTCC TCAACCTGCG CGTCGTGCAG GCATTCGGTG CGGGCATGAC 501 TGTGGTCATC GTCGGCGCAA TGGTGCGCGA TTATTATTCC GGACGCAAAG 551 CCGCCCAGAT GTTTGCCCTT ATCGGCATCA TTTTGATGGT TGTGCCGCTG 601 GTCGCACCCA TGGTCGGCGC ATTGTTGCAG GGCTTGGGTG GCTGGCAGGC 651 GATTTTTGTT TTTCTGGCGG CGTATTCGCT GGTGCTGCTC GGTTTGGTAC 701 AGTATTTCCT GCCCAAGCCC GCCGTCGGCG GCAAAATCGG CAGGGATGTG 751 TTCGGGCTGG TGGCTGGGCG GTTCAAACGC GTATTGAAAA CCCGTGCCGC 801 GATGGGTTAT CTGTTTTTTC AGGCATTCAG CTTCGGTTCG ATGTTCGCCT 851 TTCTGACCGA ATCTTCCTTC GTGTACCAGC AGCTCTACCA CGTTACGCCG 901 CACCAGTACG CTTGGGCGTT TGCACTCAAC ATCATCACGA TGATGTTTTT 951 CAACCGTATT ACCGCGTGGC GGCTCAAAAC CGGCGTGCAT CCGCAAAGCA 1001 TCCTGCTGTG GGGGATTGTC GTCCAGTTTG CCGCCAACCT GTCCCAACTC 1051 GCCGCCGTGC TGTTTTTCGG GTTGCCCCCG TTTTGGCTGC TGGTCGCGTG 1101 CGTGATGTTT TCCGTCGGTA CGCAGGGCTT GGTCGGTGCA AACACGCAGG 1151 CGTGTTTTAT GTCCTATTTC AAAGAAGAGG GCGGCAGCGC AAACGCCGTA 1201 TTGGGTGTAT TCCAATCTTT AATCGGCGCG GGGGTGGGTA TGGCGGCGAC 1251 CTTCTTGCAC GACGGTTCGG CAACCGTGAT GGCGGCAACC ATGACCGCGT 1301 CTACCTCTTG CGGCATTGCG CTTTTGTGGC TCTGCTCGCA TCGTGCGTGG 1351 AAAGAAAACG GGCAAAGCGA ATACCTTTAA This corresponds to the amino acid sequence <SEQ ID 2296; ORF 689. a>: a689.pep 1 LLIHYIVPVR PVLPGLLLPP VCAGVLKFSV SAYCVFRRRA VCLRIGREFM 51 PΞAHYPEMSE KLMAVLMAML VTLMPFSIDA YLPAIPEMAQ ΞLΝADVHRIE 101 QΞLSLFMFGT AFGQVVGGΞV ΞDIKGRKPVA LTGLAVYCLA VAAIVFASΞA 151 EQLLΝLRVVQ AFGAGMTVVI VGAMVRDYYΞ GRKAAQMFAL IGIILMVVPL 201 VAPMVGALLQ GLGGWQAIFV FLAAYΞLVLL GLVQYFLPKP AVGGKIGRDV 251 FGLVAGRFKR VLKTRAAMGY LFFQAFSFGS MFAFLTEΞSF VYQQLYHVTP 301 HQYAWAFALΝ IITMMFFΝRI TAWRLKTGVH PQSILLWGIV VQFAAΝLSQL 351 AAVLFFGLPP FWLLVACVMF SVGTQGLVGA ΝTQACFMSYF KEEGGSAΝAV 401 LGVFQSLIGA GVGMAATFLH DGΞATVMAAT MTASTSCGIA LLWLCSHRAW 451 KEΝGQSEYL* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 689 shows 99.1% identity over a 459 aa overlap with a predicted ORF (ORF 689) from N meningitidis: m689/a689 99.1% identity in 459 aa overlap 10 20 30 40 50 60 m689.pep LLIHYIVPVRPVLPGLLLPPVCAGVLKFSVΞAYCVFRRRAVCLRIGREFMPSAHYPEMSE I I I I I I I I I I I I I I I I I II I I M I I II I II I I I I I I I I I I I II I I I I I I I I I I I I I I I I I a689 LLIHYIVPVRPVLPGLLLPPVCAGVLKFSVΞAYCVFRRRAVCLRIGREFMPSAHYPEMSE 10 20 30 40 50 60 70 80 90 100 110 120 m689.pep KLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLΝADVHRIEQSLSLFMFGTAFGQVVGGSV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a689 KLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLΝADVHRIEQSLSLFMFGTAFGQVVGGSV 70 80 90 100 110 120 130 140 150 160 170 180 m689.pep SDIKGRKPVALTGLIVYCLAVAAIVFVSSAE Q LLΝLRVVQAFGAGMTVVIVGAMVRDYYS I II I II I I I I IM II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a689 SDIKGRKPVALTGLAVYCLAVAAIVFASSAEQLLΝLRVVQAFGAGMTVVIVGAMVRDYYS 130 140 150 160 170 180 190 200 210 220 230 240 m689.pep GRKAA Q MFALIGIILMVVPLVAPMVGALL Q GLGGWQAIFVFLAAYSLVLLGLVQYFLPKP I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a689 GRKAAQMFA IG11LMVVPLVAPMVGALLQGLGGWQAIFVFLAAYSLVLLG VQYFLPKP 190 200 210 220 230 240 250 260 270 280 290 300 m689.pep AVGGKIGRDVFGLVAGRFKRVLKTRAAMGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTP I I I I II I I II I I I I I I I I I I I I I I II I I I I I II I I I I I I I I I I I I I I I I I I I I I I l I I a689 AVGGKIGRDVFGLVAGRFKRVLKTRAAMGYLFFQAFSFGSMFAFLTESΞFVYQQLYHVTP 250 260 270 280 290 300 310 320 330 340 350 360 m689.pep HQYAWAFALNIITMMFFNRVTAWRLKTGVHPQSILLWGIVVQFAANLΞQLAAVLFFGLPP I I I I I I I I I I I I II I I I I l II I II I II I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I a689 HQYAWAFALNIITMMFFNRITAWRLKTGVHPQΞILLWGIVVQFAANLSQLAAVLFFGLPP 310 320 330 340 350 360 370 380 390 400 410 420 m689.pep FWLLVACVMFSVGTQGLVGANTQACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLH 111111 ii i ii 1111111111111111111111111111111111111111111111111 a689 FWLLVACVMFSVGTQGLVGANTQACFMΞYFKEEGGSANAVLGVFQSLIGAGVGMAATFLH 370 380 390 400 410 420 430 440 450 460 m689. pep DGSATVMAATMTASTSCGIALLWLCSHRAWKENGQSEYLX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a689 DGSATVMAATMTASTSCGIALLWLCSHRAWKENGQΞEYLX 430 440 450 460 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2297> g690.seq (partial) 1 ATGAAAAACA AAACGTCATC ACTTCCCTTA TGGCTTGCCG CAATCATGCT 51 GGCCGCGCGT TCCCCGAGCA AAGAAGATAA AACGAAAGAA AACGGCGCAT 101 CCGCCGCTTC GTCTTCCGCG TCATCGGCTT CTTCCCAAAC CGATTTGCAA 151 CCGGCCGCAT CCGCCCCTGA TAACGTCAAG CAGGCAGAAA GCGCGCCACT 201 GTGAAATTGC ACCGGCCTGC ACCCCGCCGC CGGCATTGGC GATCTCATAC 251 AGCAAATCGC CGAACACATC GACTCGGACT GTCTGTTTGC CCTTTCCCAT 301 AACGAACTGG AAACCCGTTT CGGCTTACCC GGCGGCGGCT ATGACAACAT 351 ACAGCGGctG CTgtttCCCG ACATCCGCCC TGAAGATCCC GACTACCATC 401 AGAAAATCAT GCTGGCAATC GAAGACTTGC GTTACGGAAC GCGCACCATC 451 AGccgGCAGG CACAAGATGC CATAATGGAA CAGGAACGCC gcctccGaGa 501 agCGACGCTG ATGCTGACAC AGGGCAGTCA AAAAACCCGC GGaCAAGGCG 551 AGGAACCGAA ACGCGCACGT TATTTTGAAG TTTCGGCAAC ATCtgCCtaT 601 TTgaaccggC ACAAcaacGG ACTTggcgGC AATTTCCAAT ACATCGGCCA 651 ATTGCCCGGC TATCTGAAAA TGCACGGAGA AATGCTTGAA AACCAATCAC 701 TCTTCCGGCT GTCCAACCGT GAACGCAATC CCGACAAACC GTTTTTAGAC 751 ATCCATTTTG ACGAAAATGG CAAAATCACG CGTATTGTCG TTTACGAAAA 801 AAATATCTAT ... This corresponds to the amino acid sequence <SEQ ID 2298; ORF 690 >: g690.pep (partial) 1 MKΝKTΞSLPL WLAAIMLAAR ΞPSKEDKTKE ΝGASAASSSA SSASSQTDLQ 51 PAASAPDΝVK QAESAPL*ΝC TGLHPAAGIG DLIQQIAEHI DSDCLFALSH 101 NELETRFGLP GGGYDNIQRL LFPDIRPEDP DYHQKIMLAI EDLRYGTRTI 151 ΞRQAQDAIME QERRLREATL MLTQGΞQKTR GQGEEPKRAR YFEVSATSAY 201 LNRHNNGLGG NFQYIGQLPG YLKMHGEMLE NQSLFRLSNR ERNPDKPFLD 251 IHFDENGKIT RIVVYEKNIY ... The following partial DNA sequence was identified in N meningitidis <SEQ ID 2299>: m690.seq.. 1 ATGAAAAACA AAACCTCATC ACTTCTCTTA TGGCTTACCG CAATCATGCT 51 GACCGCGTGT TCTCCGAGCA AAGACGATAA AACCAAAGAA GTCGGTGCAT 101 CCGCTGCTTC GTCCTCCGCG TCATCAGCTC CTTCCCAAAC CGATTTGCAA 151 CCGACCGCAT CCGCCCCTGA TAACGTCAAG CAGGCAGAAA GCGCGCCGCC 201 GTCAAATTGC ACCAGCCTGC ACCCCGCCAC CGGCATTGAC GATCTCATGC 251 AGCAAATCGC CGAACACATT GACTCGGACT GTCTGTTTGC CCTTTCCCAT 301 CACGAACTGG AAACCCGTTT CGGCTTACCC GACGGTGGCT ATGACAACAT 351 ACAGCGGCTG CTGTTTCCCG ACATCCGCCC TGAAGATCCC GACTACCATC 401 AGAAAATCAT ACTGGCAATT GAAGACTTGC GTTACGGAAA GCGCACGATC 451 AGCCGGCAGG CACAAAATGC CTTGATGGAA CAGGAACGCC GCCTCCGAGA 501 AGCGACGCTG TTGCTGATAC AGGGCAGTCA AGAAACCCGC GGACAAGGCG 551 AGGAGCCGAA ACGCACGCGT TATTTTGAAG TTTCGGCAAC CCCTGCCTAT 601 TCGAGCCGGC ACAACAACGG ACTTGGCGGC AATTTCCAAT ACATCAGCCA 651 ATTGCCCGGC TATCTGAAAA TACACGGAGA AATGCTTGAA AACCAATCAC 701 TCTTCCGGCT GTCCAACCGT GAACGCAATC CCGACAAACC GTTTTTAGAC 751 ATCCATTTTG ACGAAAATGG CAAAATCACG CGTATTGTCG TTTACGAAAA 801 AAACATCTAC TTCAATCCAA ACACGGGGCG AATATAA This corresponds to the amino acid sequence <SEQ ID 2300; ORF 690>: 690.pep 1 MKNKTSΞLLL WLTAIMLTAC SPSKDDKTKE VGASAASSSA SSAPSQTDLQ 51 PTAΞAPDNVK QAEΞAPPSNC TΞLHPATGID DLMQQIAEHI DSDCLFALSH 101 HELETRFGLP DGGYDNIQRL LFPDIRPEDP DYHQKIILAI EDLRYGKRTI 151 SRQAQNALME QERRLREATL LLIQGΞQETR GQGEEPKRTR YFEVSATPAY 201 ΞSRHNNGLGG NFQYISQLPG YLKIHGEMLE NQSLFRLSNR ERNPDKPFLD 251 IHFDENGKIT RIVVYEKNIY FNPNTGRI* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 690 shows 89.3% identity over a 408 aa overlap with a predicted ORF (ORF 690) from N gonorrhoeae: m690/g690 89.3% identity in 408 aa overlap 10 20 30 40 50 60 m690.pep MKΝKTSΞLLLWLTAIMLTACSPΞKDDKTKEVGASAASΞSASSAPSQTDLQPTASAPDΝVK l I I I : I I I I I I I M I I I I I I I I I I I I I I I I I I I I I I I I: I I I I I I I I g690 MKΝKTSSLPLWLAAIMLAARΞPSKEDKTKEΝGASAASSSASSASΞQTDLQPAAΞAPDΝVK 10 20 30 40 50 60 70 80 90 100 110 120 m690.pep QAEΞAPPSΝCTΞLHPATGIDDLMQQIAEHIDSDCLFALSHHELETRFGLPDGGYDΝIQRL llllll I I l I I Ml I I M I I I I I I I I I I I I I I I I I : I I I I I I I I I ll g690 QAESAPLXΝCTGLHPAAGIGDLIQQIAEHIDSDCLFALΞHΝELETRFGLPGGGYDΝIQRL 70 80 90 100 110 120 130 140 150 160 170 180 m690.pep LFPDIRPEDPDYHQKIILAIEDLRYGKRTIΞRQAQΝALMEQERRLREATLLLIQGΞQETR I I I I I I I I I I I I I I I l I I I I I I I I I I I I I I I I M I I I I I I I I I I Ml I I I I : I I g690 LFPDIRPEDPDYHQKIMLAIEDLRYGTRTISRQAQDAIMEQERRLREATLMLTQGΞQKTR 130 140 150 160 170 180 190 200 210 220 230 240 m690.pep GQGEEPKRTRYFEVSATPAYSΞRHΝΝGLGGΝFQYISQLPGYLKIHGEMLEΝQSLFRLSΝR I I I I I I I M I I I I I I I I II : I I I I I I I I I I I I M I I I I I I Ml I I I I I I I I I I I I I II g690 GQGEEPKRARYFEVSATSAYLΝRHΝΝGLGGΝFQYIGQLPGYLKMHGEMLEΝQSLFRLSΝR 190 200 210 220 230 240 250 260 270 279 m690.pep ERΝPDKPFLDIHFDEΝGKITRIVVYEKΝIYFΝPΝTGRIX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g690 ERΝPDKPFLDIHFDEΝGKITRIVVYEKΝIY 250 260 270 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2301> a690.seq 1 ATGAAAAACA AAACCTCATC ACTTCTCTTA TGGCTTGCCG CAATGATGCT 51 GACCGCGTGT TCCCCGAGCA AAGAAGATAA AACGAAAGAA AACGGCGCAT 101 CCGCCGCCTC GTCCACGGCA TCCGCCGCTT CGTCTTCCGC GCCCCAAACC 151 GATTTGCAAC CGGCCGCATC CGCCCCTGAT AACGTCAAGC AGGCAGAAAG 201 CGTGCCGCCG TCAAATTGCA CCGACCTGCA CCCCGCCACC GGCATTGACG 251 ATCTCATGCA GCAAATCGCC GAACACATTG ACTCGGACTG TCTGTTTGCC 301 CTTTCCCATC ACGAACTGGA AACCCGTTTC GGCTTACCCG GCGGCGGCTA 351 TGACAACATA CAGCGGCTGC TGTTTCCCGA CATCCGCCCT GAAGATCCCG 401 ACTACCATCA GAAAATCATA CTGGCAATTG AAGACTTGCG TTACGGAAAG 451 CGCACGATCA GCCGGCAGGC ACAAGATGCC TTGATGGAAC AGGAACGCCG 501 CCTCCGAGAA GCGACGCTGT TGCTGATACA GGGCAGTCAA GAAACCCGCG 551 GACAAGGCGA GGAGCCGAAA CGCACGCGTT ATTTTGAAGT TTCGGCAACC 601 CCTGCCTATT CGAGCCGGCA CAACAACGGA CTTGGCGGCA ATTTCCAATA 651 CATCGGCCAA TTGCCCGGCT ATCTGAAAAT ACACGGAGAA ATGCTTGAAA l l l l 701 ACCAATCACT CTTCCGGCTG TCCAACCGTG AACGCAATCC CGACAAACCG 751 TTTTTAGACA TCCATTTTGA CGAAAATGGC AAAATCACGC GTATTGTCGT 801 TTACGAAAAA AACATCTACT TCAATCCAAA CTTGGGGCGA AGATAA This corresponds to the amino acid sequence <SEQ ID 2302; ORF 690.a>: a690.pep 1 MKNKTΞSLLL WLAAMMLTAC SPSKEDKTKE NGASAASΞTA SAASSSAPQT 51 DLQPAASAPD NVKQAESVPP SNCTDLHPAT GIDDLMQQIA EHIDSDCLFA 101 LΞHHELETRF GLPGGGYDNI QRLLFPDIRP EDPDYHQKII LAIEDLRYGK 151 RTIΞRQAQDA LMEQERRLRE ATLLLIQGΞQ ETRGQGEEPK RTRYFEVSAT 201 PAYSSRHNNG LGGNFQYIGQ LPGYLKIHGE MLENQSLFRL SNRERNPDKP 251 FLDIHFDENG KITRIVVYEK NIYFNPNLGR R* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 690 shows 93.9% identity over a 280 aa overlap with a predicted ORF (ORF 690) from N meningitidis: m690/a690 93.9% identity in 280 aa overlap 10 20 30 40 50 m690.pep MKΝKTΞΞLLLWLTAIMLTACSPSKDDKTKEVGASAAΞSSAΞSAPS QTDLQPTASAPD I I I I I I I I I I I MI I I I I I I I l I I I I I I I I I I l l I I I I I I Ml I I I I a690 MKΝKTSΞLLLWLAAMMLTACSPSKEDKTKEΝGASAASSTASAAΞSSAPQTDLQPAASAPD 10 20 30 40 50 60 60 70 80 90 100 110 m690.pep ΝVKQAEΞAPPSΝCTSLHPATGIDDLMQQIAEHIDSDCLFALΞHHELETRFGLPDGGYDΝI I I I I I I Mil II I l I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a690 ΝVKQAESVPPSΝCTDLHPATGIDDLMQQIAEHIDΞDCLFALSHHELETRFGLPGGGYDΝI 70 80 90 100 110 120 120 130 140 150 160 170 m690.pep QRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQΝALMEQERRLREATLLLIQGSQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I a690 QRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTIΞRQAQDALMEQERRLREATLLLIQGSQ 130 140 150 160 170 180 180 190 200 210 220 230 m690.pep ETRGQGEEPKRTRYFEVΞATPAYΞSRHΝΝGLGGΝFQYIΞQLPGYLKIHGEMLEΝQSLFRL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M I I I I I I I I I I I I I I I I I I I a690 ETRGQGEEPKRTRYFEVSATPAYSSRHΝΝGLGGΝFQYIGQLPGYLKIHGEMLEΝQSLFRL 190 200 210 220 230 240 240 250 260 270 279 m690.pep SΝRERΝPDKPFLDIHFDEΝGKITRIVVYEKΝIYFΝPΝTGRIX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II a690 ΞΝRERΝPDKPFLDIHFDEΝGKITRIVVYEKΝIYFΝPΝLGRRX 250 260 270 280 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 2303> gδθl.seq 1 GTGCCGCTGC CTGCTCCCTG CCGTTTTGCC AAACCTGCCG CCTCTTTTTT 51 AAGTATGGCT TTGCTTTCCT GCCAGCTTTC CCACGCCGCC ACGGCTTATA 101 TCCCCCCGAA CGATTTTCAA CCGAACTGCG ACATACGCCG GCTCGGGCTG 151 ACACAGGGTC AGCACAATGA GCTGCGTAAA ATCCGCGCCG CCTTCAAAAT 201 GGCGGGCGAC AGGGCGCGTT TGAAGGTTAT GCATTCCGAA CACAGCCGCC 251 GCCGCTCTGT CGTCGAAATC ATTTCTTCGG ATGTTTTTAA TCGGAACGAG 301 GCGCGCGATT ATGTCGAAAG CCGCTACCAC TCCAGCATGG ATTTTGCGGT 351 GGACGAATTG GAAATCCAAC ACCGCTTCTT CCATATTCTC ACACCGCAAC 401 AGCAGCAAAT GTGGCTTTCT TCCTGCCTCA AATAA This corresponds to the amino acid sequence <SEQ ID 2304; ORF 691 >: g691.pep 1 VPLPAPCRFA KPAASFLSMA LLSCQLSHAA TAYIPPΝDFQ PΝCDIRRLGL 51 TQGQHΝELRK IRAAFKMAGD RARLKVMHSE HSRRRSVVEI ISSDVFΝRΝE 101 ARDYVESRYH SSMDFAVDEL EIQHRFFHIL TPQQQQMWLS ΞCLK* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2305>: 691.seq 1 GTGCCACTGC CTGCTCCCTG CCGTTTTGCC AAACCTGCCG CCTCTTTTTT 51 AAGTATGGCT TTGCTTTCCT GTCAGCTTTC CCACGCCGCC ACGGCTTATA 101 TCCCCCCGAA CGATTTTCAA CCGAACTGCG ACATACGCCG ACTCGGGCTG 151 ACCCAAAGTC AGCACAATGA GCTGCGTAAA ATCCGCACCG CCTTCAAAAT 201 GGCGGGCGAC AGGGCGCGTT TGAAGGTTAT GCATTCCGAA CACAGCCGCC 251 GCCGGTCTGT CGTCGAAATC ATTTCCTCGG ATGTTTTTAA TCGGAACGAG 301 GCGCGCGATT ATGTCGAAAG CCGCTATTTG TCCGGTATGG ATTTTGCGGT 351 GGACGAATTG GAAATCCAAC ACCGGTTCTT CCATATCCTC ACACCGCAAC 401 AGCAGCAAAT GTGGCTTTCT TCCTGCCTCA AATAA This corresponds to the amino acid sequence <SEQ ID 2306; ORF 691>: m691.pep 1 VPLPAPCRFA KPAASFLSMA LLSCQLΞHAA TAYIPPΝDFQ PΝCDIRRLGL 51 TQΞQHΝELRK IRTAFKMAGD RARLKVMHSE HSRRRSVVEI ISΞDVFΝRΝE 101 ARDYVESRYL SGMDFAVDEL EIQHRFFHIL TPQQQQMWLS SCLK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 691 shows 97.2% identity over a 144 aa overlap with a predicted ORF (ORF 691) from N gonorrhoeae: m691/g691 97.2% identity in 144 aa overlap 10 20 30 40 50 60 m691.pep VPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPPΝDFQPΝCDIRRLGLTQSQHΝELRK I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I l I I I I I I g691 VPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPPΝDFQPΝCDIRRLGLTQGQHΝELRK 10 20 30 40 50 60 70 80 90 100 110 120 m691.pep IRTAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFΝRΝEARDYVESRYLSGMDFAVDEL I Ml I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I l I I I I I I I g691 IRAAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFΝRΝEARDYVESRYHSSMDFAVDEL 70 80 90 100 110 120 130 140 m691.pep EIQHRFFHILTPQQQQMWLSSCLKX I I I I I I I I I I I I I I I I I I I I I I I I I g691 EIQHRFFHILTPQQQQMWLSSCLKX 130 140 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2307> a691.seq 1 GTGCCACTGC ΝTGCTCCCTG CCGTTTTGCC AAACCTGCCG CCTCTTTTTT 51 AAGTATGGCT TTGCTTTCCT GCCAGCTTTC CCACGCCGCC ACGGCTTATA 101 TCCCCCTGAA CGATTTTCAA CCGAACTGCG ACATACGCCG GCTCGGACTG 151 ACACAGGGTC AGCACAATGA ACTGCGTAAA ATCCGCGCCG CCTTCAAAAT 201 GGCGGGCGAC AGGGCGCGTT TGAAGGTTAT GCATTCCGAA CACAGCCGCC 251 GTCGGTCTGT CGTCGAAATC ATTTCCTCGG ATGTTTTTAA TCGGAACGAG 301 GCGCGCGATT ATGTCGAAAG CCGCTATTTG TCCGGTATGG ATTTTGCGGT 351 GGACGAATTG GAAATCCAAC ACCGGTTCTT CCATATCCTC ACACCGCAAC 401 AGCAGCAAAT GTGGCTTTCT TCCTGCCTCA AATAA This corresponds to the amino acid sequence <SEQ ID 2308; ORF 691. a>: a691.pep 1 VPLXAPCRFA KPAASFLSMA LLΞCQLSHAA TAYIPLΝDFQ PΝCDIRRLGL 51 TQGQHΝELRK IRAAFKMAGD RARLKVMHSE HSRRRSVVEI IΞSDVFΝRΝE 101 ARDYVESRYL SGMDFAVDEL EIQHRFFHIL TPQQQQMWLS SCLK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 691 shows 97.2% identity over a 144 aa overlap with a predicted ORF (ORF 691) from N. meningitidis: m691/a691 97.2% identity in 144 aa overlap 10 20 30 40 50 60 m691.pep VPLPAPCRFAKPAASFLΞMALLSCQLSHAATAYIPPΝDFQPΝCDIRRLGLTQSQHΝELRK III I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I a691 VPLXAPCRFAKPAAΞFLSMALLSCQLSHAATAYIPLΝDFQPΝCDIRRLGLTQGQHΝELRK 10 20 30 40 50 60 70 80 90 100 110 120 m691.pep IRTAFKMAGDRARLKVMHSEHΞRRRSVVEIIΞSDVFΝRΝEARDYVESRYLSGMDFAVDEL I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a691 IRAAFKMAGDRARLKVMHSEHSRRRΞVVEIIΞSDVFΝRΝEARDYVESRYLSGMDFAVDEL 70 80 90 100 110 120 130 140 m691.pep EIQHRFFHILTPQQQQMWLSSCLKX I I I I I I I I I I I I I I I I I I I I II I I I a691 EIQHRFFHILTPQQQQMWLSSCLKX 130 140 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 2309> g692.seq 1 GTATCGCACA CACGCTGTCG CTGTTCGGAA TCGAtacGCC GGATTTGGCG 51 GAATGGCAGG GAATGGCGGA TTAAAGGACA AAAATGCCGT CTGAACACGG 101 ATGCGGTTCA GACGGCATCA TTTTATACGA CTGCCTTATT TGGCTGCGCC 151 TTCATTCCAT GCGGCAGGGT ATTTGTAGCC CTCGAAGCGT TTGTGCGCGT 201 AGGCTTTGAA CGCGTCGGAG TTATAGGCCT CGGTTACGTC TTTAAGCCAT 251 TGGCTGTCTT TGTCGGCGGT TTTgacGGCA GACCAGTTGA CATAGGCAAA 301 GCTCGGCTCT TGGAACAGGG CTTCGGTCAG CTTCATGCCG CTGCTTATGG 351 CGTAGTTGCC GTTGACGACG GCAAAATCCA CGTCGGCGCG GCTGCGCGGC 401 AGTTGTGCGG CTTCAAGCTC GACGATTTTG ATGTTTTTCA GGTTTTCCGC 451 GATGTCGGCT TTGGATGCGG TCAGCGGATT GATGCCGTCT TTGAGTTTGA 501 TCCAACCCAG TTCGTTCAGC ATCACCAAGG CGCGTGCGAA GTTGGAcggG 551 TcgtTGGGCG CGGATACGGT GCTGCCGTCT TTGACTTCTT CCAGCGATTT 601 CAGTTTGCCC GGATACAGTC CCAAAGGCGC GGTCGGCACT TGGAAGGCTT 651 CGGTGATGTC CAGGTTGTGT TCTTTTTTGA AATCGTCAAG ATAGGGTTTG 701 TGTTGGAAGA CGTTGATGTC CAACTCGCCC TCCGCCAATG CCAGATTCGG 751 GCGCACATAG TCggTAAATT cgaccaatTT gacgGTGTag CCTTTTTTCT 801 CCAGCTCGgc tTGGATTTGT TCTTTGACCA TATcgccgaa gtcgcccacg 851 gTCGTGCCGA agacgaTTTC TTTTTTCGCC GcgcCGTTAT CGGCAGAAGG 901 GGCGGCGgca gaggctgcGG GCGCGCTGTC TTTTtgaccG ccgCAGGCTG 951 CGAGGATGAG CGCGAGtgcg gcggcggaaa ggGTTTTGAA GAAGGTTTTc 1001 atATTTTCTc ctga This corresponds to the amino acid sequence <SEQ ID 2310; ORF 692 >: g692.pep 1 VSHTRCRCΞE SIRRIWRΝGR EWRIKGQKCR LΝTDAVQTAS FYTTALFGCA 51 FIPCGRVFVA LEAFVRVGFE RVGVIGLGYV FKPLAVFVGG FDGRPVDIGK 101 ARLLEQGFGQ LHAAAYGVVA VDDGKIHVGA AARQLCGFKL DDFDVFQVFR 151 DVGFGCGQRI DAVFEFDPTQ FVQHHQGACE VGRVVGRGYG AAVFDFFQRF 201 QFARIQSQRR GRHLEGFGDV QVVFFFEIVK IGFVLEDVDV QLALRQCQIR 251 AHIVGKFDQF DGVAFFLQLG LDLFFDHIAE VAHGRAEDDF FFRRAVIGRR 301 GGGRGCGRAV FLTAAGCEDE RECGGGKGFE EGFHIFS* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2311>: m692. seq 1 GTGTTGCACA CGCTTTGTCG CTGTTCGGAA TCGATACGCC GGATTCGGCG 51 GAATGGCAGG GAATGGCGGA TTAAAGGACA AAAATGCCGT CTGAACACGG 101 ATACAGTTCA GACGGCATCA TTTTATACGA CTGCCTTATT TGGCTGCGCC 151 TTCATTCCAT GCGGCAGGGG ATTTGTAGCC CTCGAAGCGT TTGTGCGCGT 201 AGGCTTTGAA CGCGTCGGAG TTATAGGCCT CGGTTACGTC TTTAAGCCAT 251 TGGCTGTCTT TGTCGGCGGT TTTGACGGCA GACCAGTTGA CATAGGCAAA 301 GCTCGGTTCT TGGAACAGGG CTTCGGTCAG CTTCATGCCG CTGCTTATGG 351 CGTAGTTGCC GTTGACGACG GCAAAATCCA CGTCGGCGCG GCTACGCGGC 401 AGTTGCGCGG CTTCAAGCTC GACGATTTTG ATGTTTTTCA GGTTCTCGGC 451 GATGTCCGCT TTGGATGCGG TCAACGGATT GATGCCGTCT TTGAGTTTGA 501 TCCAACCCAG TTCGTCGAGC ATCACCAAGA CGCGGGCGAA GTTGGACGGG 551 TCGTTGGGCG CGGATACGGT GCTGCCGTCT TTGACTTCTT CCAGCGATTT 601 CAGCTTGCCC GGGTACAGTC CCAAAGGCGC GGTCGGCACT TGGAAGACTT 651 CGGTGATGTC CAGATTGTGT TCTTTTTTGA AGTCGTCAAG ATAGGGTTTG 701 TGTTGGAAGA CGTTGATGTC CAACTCGCCC TCAGCCAATG CCAGATTCGG 751 GCGTACATAG TCGGTAAACT CGACCAGTTT GACGGTGTAG CCTTTTTTCT 801 CCAGCTCGGC TTGGATTTGT TCTTTGACCA TATCGCCGAA GTCGCCGACG 851 GTCGTGCCGA AGACGATTTC TTTTTTCGCC GCGCCGTTGT CGGCGGCGGC 901 AGAAGCGGAT GCGGCGGGCG CGCTGTCTTT TTGACCGCCG CAGGCGGCGA 951 GGATGAGCGC GAGTGCGGCG GCGGAAAGGG TTTTGAAGAA GGTTTTCATA 1001 TTTTCTCCTG A This corresponds to the amino acid sequence <SEQ ID 2312; ORF 692>: m692.pep 1 VLHTLCRCΞE SIRRIRRNGR EWRIKGQKCR LNTDTVQTAS FYTTALFGCA 51 FIPCGRGFVA LEAFVRVGFE RVGVIGLGYV FKPLAVFVGG FDGRPVDIGK 101 ARFLEQGFGQ LHAAAYGVVA VDDGKIHVGA ATRQLRGFKL DDFDVFQVLG 151 DVRFGCGQRI DAVFEFDPTQ FVEHHQDAGE VGRVVGRGYG AAVFDFFQRF 201 QLARVQSQRR GRHLEDFGDV QIVFFFEVVK IGFVLEDVDV QLALSQCQIR 251 AYIVGKLDQF DGVAFFLQLG LDLFFDHIAE VADGRAEDDF FFRRAVVGGG 301 RSGCGGRAVF LTAAGGEDER ECGGGKGFEE GFHIFS* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 692 shows 91.1% identity over a 338 aa overlap with a predicted ORF (ORF 692) from N gonorrhoeae: m692/g692 91.1% identity in 338 aa overlap 10 20 30 40 50 60 m692.pep VLHTLCRCSESIRRIRRΝGREWRIKGQKCRLΝTDTVQTAΞFYTTALFGCAFIPCGRGFVA I II I I I I I I I I I I I I I I I I I I M I I I I I I I I I I I I I I I I I I I I III g692 VSHTRCRCSESIRRIWRΝGREWRIKGQKCRLΝTDAVQTASFYTTALFGCAFIPCGRVFVA 10 20 30 40 50 60 70 80 90 100 110 120 m692. pep LEAFVRVGFERVGVIGLGYVFKPLAVFVGGFDGRPVDIGKARFLEQGFGQLHAAAYGVVA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I l I I I I I I I I I I I I I I I I g692 LEAFVRVGFERVGVIGLGYVFKPLAVFVGGFDGRPVDIGKARLLEQGFGQLHAAAYGVVA 70 80 90 100 110 120 130 140 150 160 170 180 m692. ,pep VDDGKIHVGAATRQLRGFKLDDFDVFQVLGDVRFGCGQRIDAVFEFDPTQFVEHHQDAGE I I I I I I I I I I I :| I I I I I I I I I I I I I I : II I I I I I I I I I I I I I I I I I I I : I I I I I g692 VDDGKIHVGAAARQLCGFKLDDFDVFQVFRDVGFGCGQRIDAVFEFDPTQFVQHHQGACE 130 140 150 160 170 180 190 200 210 220 230 240 m692, .pep VGRVVGRGYGAAVFDFFQRFQLARVQSQRRGRHLEDFGDVQIVFFFEVVKIGFVLEDVDV II I I I I I II I I I I II I II I I Ml Ml I I I I I I I I I I I I I Ml I I I IM I I I I I I I I I I I g692 VGRVVGRGYGAAVFDFFQRFQFARIQSQRRGRHLEGFGDVQVVFFFEIVKIGFVLEDVDV 190 200 210 220 230 240 250 260 270 280 290 m692, .pep QLALΞQCQIRAYIVGKLDQFDGVAFFLQLGLDLFFDHIAEVADGRAEDDFFFRRAVVG— llll I I I I I I : I I I M I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I g692 QLALRQCQIRAHIVGKFDQFDGVAFFLQLGLDLFFDHIAEVAHGRAEDDFFFRRAVIGRR 250 260 270 280 290 300 300 310 320 330 m692 .pep GGRSGCGGRAVFLTAAGGEDERECGGGKGFEEGFHIFSX II III ll I I I I I I I I I I I I I I I I I I I I I g692 GGGRGCG-RAVFLTAAGCEDERECGGGKGFEEGFHIFSX 310 320 330 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2313> a692.seq 1 GTGTTGCACA CGCTTTGTCG CTGTTCGGAA TCGATACGCC GGATTCGGCG 51 GAATGGCAGG GAATGGCGGA TTAAAGGACA AAAATGCCGT CTGAACACGG 101 ATACGGTTCA GACGGCATCA TTTTATACGA CTGCCTTATT TGGCTGCGCC 151 TTCATTCCAT GCGGCAGGGG ATTTGTAGCC CTCGAAGCGT TTGTGCGCGT 201 AGGCTTTGAA CGCGTCGGAG TTATAGGCCT CGGTTACGTC TTTAAGCCAT 251 TGGCTGTCTT TGTCGGCGGT TTTGACGGCA GACCAGTTGA CATAGGCAAA 301 GCTCGGTTCT TGGAACAGGG CTTCGGTCAG CTTCATGCCG CTGCTTATGG 351 CGTAGTTGCC GTTGACGACG GCAAAATCCA CGTCGGCGCG GCTACGCGGC 401 AGTTGCGCGG CTTCAAGCTC GACGATTTTG ATGTTTTTCA GGTTTTCGGC 451 AATGTCCGCT TTGGATGCGG TCAGCGGATT GATGCCGTCT TTGAGTTTGA 501 TCCAACCCAG TTCGTCGAGC ATCACCAAGA CGCGGGCGAA GTTGGACGGG 551 TCGTTGGGCG CGGATACGGT GCTGCCGTCT TTGACTTCTT CCAGCGATTT 601 CAGCTTGCCC GGGTACAGTC CCAAAGGCGC GGTCGGCACT TGGAAGACTT 651 CGGTGATGTC CAGATTGTGT TCTTTTTTGA AGTCGTCAAG ATAGGGTTTG 701 TGTTGGAAGA CGTTGATGTC CAACTCGCCC TCAGCCAATG CCAGATTCGG 751 GCGCACATAG TCGGTAAACT CGACCAGTTT GACGGTGTAG CCTTTTTTCT 801 CCAGCTCGGG TTGGATTTGT TCTTTGACCA TATCGCCGAA GTCGCCGACG 851 GTCGTGCCGA AGACGATTTC TTTTTTCGCC GCGCCGTTGT CGGCGGCGGC 901 AGAAGCGGAT GCGGCGGGCG CGCTATCTTT TTGACCGCCG CAGGCGGCGA 951 GGATGAGCGC GAGTGCGGCG GCGGAAAGGG TTTTGAAGAA GGTTTTCATA 001 TTTTCTCCTG A This corresponds to the amino acid sequence <SEQ ID 2314; ORF 692.a>: a692.pep 1 VLHTLCRCSE SIRRIRRNGR EWRIKGQKCR LNTDTVQTAS FYTTALFGCA 51 FIPCGRGFVA _LEAFVRVGFE RVGVIGLGYV FKPLAVFVGG FDGRPVDIGK 101 ARFLEQGFGQ LHAAAYGVVA VDDGKIHVGA ATRQLRGFKL DDFDVFQVFG 151 NVRFGCGQRI DAVFEFDPTQ FVEHHQDAGE VGRVVGRGYG AAVFDFFQRF 201 QLARVQSQRR GRHLEDFGDV QIVFFFEVVK IGFVLEDVDV QLALSQCQIR 251 AHIVGKLDQF DGVAFFLQLG LDLFFDHIAE VADGRAEDDF FFRRAVVGGG 301 RSGCGGRAIF LTAAGGEDER ECGGGKGFEE GFHIFS* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 692 shows 98.8% identity over a 336 aa overlap with a predicted ORF (ORF 692) from N meningitidis: 692/a692 98.8% identity in 336 aa overlap 10 20 30 40 50 60 m692. ,pep VLHTLCRCSESIRRIRRΝGREWRIKGQKCRLΝTDTVQTASFYTTALFGCAFIPCGRGFVA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a692 VLHTLCRCSEΞIRRIRRΝGREWRIKGQKCRLΝTDTVQTAΞFYTTALFGCAFIPCGRGFVA 10 20 30 40 50 60 70 80 90 100 110 120 m692. .pep LEAFVRVGFERVGVIGLGYVFKPLAVFVGGFDGRPVDIGKARFLEQGFGQLHAAAYGVVA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a692 LEAFVRVGFERVGVIGLGYVFKPLAVFVGGFDGRPVDIGKARFLEQGFGQLHAAAYGVVA 70 80 90 100 110 120 130 140 150 160 170 180 m692, •pep VDDGKIHVGAATRQLRGFKLDDFDVFQVLGDVRFGCGQRIDAVFEFDPTQFVEHHQDAGE I I I I I I I I I I I I I I I I I I I I I I I I I I I M I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a692 VDDGKIHVGAATRQLRGFKLDDFDVFQVFGΝVRFGCGQRIDAVFEFDPTQFVEHHQDAGE 130 140 150 160 170 180 190 200 210 220 230 240 m692.pep VGRVVGRGYGAAVFDFFQRFQLARVQSQRRGRHLEDFGDVQIVFFFEVVKIGFVLEDVDV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a692 VGRVVGRGYGAAVFDFFQRFQLARVQSQRRGRHLEDFGDVQIVFFFEVVKIGFVLEDVDV 190 200 210 220 230 240 250 260 270 280 290 300 m692.pep QLALSQCQIRAYIVGKLDQFDGVAFFLQLGLDLFFDHIAEVADGRAEDDFFFRRAVVGGG I I I I I I I I I I I M I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a692 QLALSQCQIRAHIVGKLDQFDGVAFFLQLGLDLFFDHIAEVADGRAEDDFFFRRAVVGGG 250 260 270 280 290 300 310 320 330 m692.pep RSGCGGRAVFLTAAGGEDERECGGGKGFEEGFHIFSX I I II I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I a692 RSGCGGRAIFLTAAGGEDERECGGGKGFEEGFHIFSX 310 320 330 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2315>: g694.seq 1 TCGGCATTTG TGTTGCCCAA ACATCCGATG CCTGCGTTAA CGCCTGCGTC 51 AACGTTTGCA CAAATCGGGT TTGGTTTCGC CCTCGCGGCG CAGCTCCTTG 101 GGCAGGACGA ACACGATGCT TTCTTCCGCG CCCCCCCCTT CGCGCACGGT 151 TTCATGCCCC CATCCGCGTA TGGTTGCCAA TACTTCCCGC ACCAACACTT 201 CGGGCGCGGA CGCGCCTGCC GTTACGCCGA CTTTGCTTTT GCCTTCAAAC 251 CACGTGCGTT GCaggTAGGA CGCGTTGTCC ACCATATACG CATCGATTCC 301 GCGCGATGCC GCCACTTCGC GCAGGCGGTT GCTGTTGGAC GAATTGGGCG 351 AACCGACCAC AATCACGATG TCGCACTGTT CCGCCAGCTC TTTGACGGCG 401 GTTTGCCGGT TGGTCGTCGC ATAGCAGATG TCTTCCTTGT GCGGATTGCG 451 GATATTGGGG AAACGCGCGT TCAGCGCGGC GATGATGTCT TTGGTTTCAT 501 CGACCGAGAG CGTGGTTTGG CTGACATAGG CGAGTTTGTC GGGGTTTCTG 551 ACTTCGAGTT TTGCCACATC TCCGACCGTT TCGACCAAAA GCATTTTGCC 601 CGGTGCAAGC TGCCCCATCG TGCCTTCGAC CTCGGCGTGC CCCTTATGCC 651 CGATCATGAT GATTTCACAG TCTTGGGCAT CCAGTCGGGC GACTTCCTTA 701 TGCACTTTCG TCACCAGCGG GCAAGTCGCA TCAAATACCC GGAAACCGCG 751 CTCCGCCGCT TCCTGCTGCA CCGCCTTCGA TACGCCGTGT GCCGAATAAA 801 CCAGTGTCGC GCCCGGCGGC ACTTCCGCCA AGTCTTCGAT AAACACCGCG 851 CCTTTTTCGC GCAGGTTGTC CACGACGAAT TTGTTGTGGA CGACTTCGTG 901 GCGCACATAA ACCGGCGCGC CGAATTCTTC CAAAGCACGT TCGACAATAC 951 TGATTGCCCG ATCCACACCG GCGCAGAAGC CGCGCGGATT GGCAAGGATG 1001 ATGGTTTTTC CGTTCATAAG TTTTGCATTC CGTGTTCAGA CGGCATTCAC 1051 GTTTTTTTGC TΝΝATCTTTG CGATGGACGA TATTGTCAAG CACCGCCAAC 1101 ACCGCACCGA CGCAGATAA This corresponds to the amino acid sequence <SEQ ID 2316; ORF 694 >: g694.pep (partial) 1 SAFVLPKHPM PALTPASTFA QIGFGFALAA QLLGQDEHDA FFRAPPFAHG 51 FMPPΞAYGCQ YFPHQHFGRG RACRYADFAF AFKPRALQVG RVVHHIRIDS 101 ARCRHFAQAV AVGRIGRTDH ΝHDVALFRQL FDGGLPVGRR IADVFLVRIA 151 DIGETRVQRG DDVFGFIDRE RGLADIGEFV GVSDFEFCHI SDRFDQKHFA 201 RCKLPHRAFD LGVPLMPDHD DFTVLGIQSG DFLMHFRHQR ASRIKYPETA 251 LRRFLLHRLR YAVCRIΝQCR ARRHFRQVFD KHRAFFAQVV HDEFVVDDFV 301 AHIΝRRAEFF QΞTFDΝTDCP IHTGAEAARI GKDDGFSVHK FCIPCΞDGIH 351 VFLLXLCDGR YCQAPPTPHR RR* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2317>: 694.seq 1 TTGGTTTCCG CATCCGGCAC ACGGCAAAAA TGCCGTCTGA AGCCTGTTCA 51 GACGGCATTT GTGTTGCCCA AACATTCAAC GCCTGCGTCA ACGTTTGCAC 101 AAATCGGGTT TGGTTTCGCC CTCGCGGCGC AACTCTTTGG GCAGGACGAA 151 CACAATGCTT TCTTCCGCAC CCTCGCCTTC GCGTACGGTT TCGTGCCCCC 201 ATCCGCGTAT GGTTGCCAGT ACTTCCCGCA CCAACACTTC GGGCGCGGAC 251 GCGCCTGCCG TTACGCCGAC TTTGTTTTTG CCCTCAAACC ATGCGCGTTG 301 CAGGTAGCCT GCATTATCCA CCATATACGC ATCGATTCCG CGCGATGCCG 351 CCACTTCGCG CAAGCGGTTG CTGTTGGACG AATTGGGCGA ACCGACCACA 401 ATCACGATGT CGCACTGTTC TGCCAACTCT TTGACGGCGG TTTGCCGGTT 451 GGTCGTCGCA TAGCAGATAT CTTCCTTGTG CGGATTGCGG ATATTGGGGA 501 AACGCGCGTT CAGCGCGGCG ATGATGTCTT TGGTTTCATC GACCGAGAGC 551 GTGGTTTGGC TGACATAGGC GAGTTTGTCG GGGTTTCTGA CTTCGAGTTT 601 TGCCACATCT CCGACCGTTT CGACCAAAAG CATTTTGCCC GGCGCAAGCT 651 GCCCCATCGT TCCTTCGACC TCGACGTGCC CCTTATGCCC GATCATGATG 701 ATTTCACAGT CTTGGGCATC CAGTCGGGCG ACTTCCTTAT GCACTTTCGT 751 CACCAGCGGG CAAGTCGCAT CAAACACGCG GAAACCGCgC TCCGCCGCTT 801 CTTGCCGCAC CGCCTTCGAT ACGCCGTGTG CCGAATAAAC CAGTGTCGCG 851 CCCGGCGGCA CTTCCGCCAA GTCTTCAATA AACACCGCAC CTTTTTCACG 901 CAGGTTGTCC ACGACGAATT TGTTGTGAAC GACTTCGTGG CGCACATAAA 951 TCGGCGCGCC GAACTCTTCC AAAGCACGTT CGACAATACT GATT GCCCGA 1001 TCCACACCAG CGCAGAAGCC GCGCGGATTG GCAAGGATGA TGGTTTTCTC 1051 GTTCATAAGC CCGGTATTTC GTTTTCAGAC GGCATCAATA TTTTTCTTCT 1101 TGGGTTTTAC GGTGGACGAT GTTGTCCAAC ACCGCCAACA CCGCACCGAC 1151 GCAGATAA This corresponds to the amino acid sequence <SEQ ID 2318; ORF 694>: m694.pep 1 LVSASGTRQK CRLKPVQTAF VLPKHSTPAS TFAQIGFGFA LAAQLFGQDE 51 HΝAFFRTLAF AYGFVPPSAY GCQYFPHQHF GRGRACRYAD FVFALKPCAL 101 QVACIIHHIR IDSARCRHFA QAVAVGRIGR TDHΝHDVALF CQLFDGGLPV 151 GRRIADIFLV RIADIGETRV QRGDDVFGFI DRERGLADIG EFVGVSDFEF 201 CHISDRFDQK HFARRKLPHR SFDLDVPLMP DHDDFTVLGI QSGDFLMHFR 251 HQRASRIKHA ETALRRFLPH RLRYAVCRIN QCRARRHFRQ VFNKHRTFFT 301 QVVHDEFVVN DFVAHINRRA ELFQSTFDNT DCPIHTSAEA ARIGKDDGFL 351 VHKPGISFSD GINIFLLGFY GGRCCPTPPT PHRRR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 694 shows 86.8% identity over a 372 aa overlap with a predicted ORF (ORF 694) from N gonorrhoeae: 694/g694 86.8% identity in 372 aa overlap 10 20 30 40 50 m694. pep LVSASGTRQKCRLKPVQTAFVLPKHS TPAΞTFAQIGFGFALAAQLFGQDEHΝAFFR : I I I I I I I I I I I I I I I I I I I I I I I I I M I I I I M I I I I g694 SAFVLPKHPMPALTPASTFAQIGFGFALAAQLLGQDEHDAFFR 10 20 30 40 60 70 80 90 100 110 m694.pep TLAFAYGFVPPSAYGCQYFPHQHFGRGRACRYADFVFALKPCALQVACIIHHIRIDSARC : I Ml Ml I I I I I I I I I I I I I I I I I I I I I I I MM Ml I IMM ::l I I I I I I I I I g694 APPFAHGFMPPSAYGCQYFPHQHFGRGRACRYADFAFAFKPRALQVGRVVHHIRIDΞARC 50 60 70 80 90 100 120 130 140 150 160 170 m694.pep RHFAQAVAVGRIGRTDHΝHDVALFCQLFDGGLPVGRRI DIFLVRIADIGETRVQRGDDV I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M I I I I I I I I I I I I I I I I I I I g694 RHFAQAVAVGRIGRTDHΝHDVALFRQLFDGGLPVGRRIADVFLVRIADIGETRVQRGDDV 110 120 130 140 150 160 180 190 200 210 220 230 m694.pep FGFIDRERGLADIGEFVGVSDFEFCHISDRFDQKHFARRKLPHRSFDLDVPLMPDHDDFT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M I I I I I I I I I I I I I I g694 FGFIDRERGLADIGEFVGVSDFEFCHISDRFDQKHFARCKLPHRAFDLGVPLMPDHDDFT 170 180 190 200 210 220 240 250 260 270 280 290 m694.pep VLGIQSGDFLMHFRHQRASRIKHAETALRRFLPHRLRYAVCRIΝQCRARRHFRQVFΝKHR I I I I I I I I I I I I I I I I I I I I I M I I I I I I I I I I I I I I I I I I I I I I I :| I I g694 VLGIQSGDFLMHFRHQRASRIKYPETALRRFLLHRLRYAVCRIΝQCRARRHFRQVFDKHR 230 240 250 260 270 280 300 310 320 330 340 350 m694.pep TFFTQVVHDEFVVΝDFVAHIΝRRAELFQSTFDΝTDCPIHTSAEAARIGKDDGFLVHKPGI : I M I I I I I I I I M I I I I I I I I I I I M I I I I I I I I I I I II : I I I I I I I I I I I I III I g694 AFFAQVVHDEFVVDDFVAHIΝRRAEFFQSTFDΝTDCPIHTGAEAARIGKDDGFSVHKFCI 290 300 310 320 330 340 360 370 380 m694.pep SFSDGIΝIFLLGFYGGRCCPTPPTPHRRRX II I I ::| I : II I M I I I I I I I I g694 PCSDGIHVFLXXLCDGRYCQAPPTPHRRRX 350 360 370 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2319>: a694.seq 1 TTGGTTTCCG CATCCGGCAC ACGGCAAAAA TGCCGTCTGA AGCCTGTTCA 51 GACGGCATTT GTGTTGCCCA AACATTCAAC GCCTGCGTCA ACGTTTGCAC 101 AAATCGGGTT TGGTTTCGCC CTCGCGGCGC AACTCTTTGG GCAGGACGAA 151 CACAATGCTT TCTTCCGCAC CCTCGCCTTC GCGTACGGTT TCGTGCCCCC 201 ATCCGCGTAT GGTTGCCAGT ACTTCCCGCA CCAACACTTC GGGCGCGGAC 251 GCGCCTGCCG TTACGCCGAC TTTGTTTTTG CCCTCAAACC ATGCGCGTTG 301 CAGGTAGCCT GCATTATCCA CCATATACGC ATCGATTCCG CGCGATGCCG 351 CCACTTCGCG CAAGCGGTTG CTGTTGGACG AATTGGGCGA ACCGACCACA 401 ATCACGATGT CGCACTGTTC TGCCAACTCT TTGACGGCGG TTTGCCGGTT 451 GGTCGTCGCA TAGCAGATAT CTTCCTTGTG CGGATTGCGG ATATTGGGGA 501 AACGCGCGTT CAGCGCGGCG ATGATGTCTT TGGTTTCATC GACCGAGAGC 551 GTGGTTTGGC TGACATAGGC GAGTTTGTCG GGGTTTCTGA CTTCGAGTTT 601 TGCCACATCT CCGACCGTTT CGACCAAAAG CATTTTGCCC GGCGCAAGCT 651 GCCCCATCGT TCCTTCGACC TCGACGTGCC CCTTATGCCC GATCATGATG 701 ATTTCACAGT CTTGGGCATC CAGTCGGGCG ACTTCCTTAT GCACTTTCGT 751 CACCAGCGGG CAAGTCGCAT CAAACACGCG GAAACCGCGC TCCGCCGCTT 801 CTTGCCGCAC CGCCTTCGAT ACGCCGTGTG CCGAATAAAC CAGTGTCGCG 851 CCCGGCGGCA CTTCCGCCAA GTCTTCAATA AACACCGCAC CTTTTTCACG 901 CAGGTTGTCC ACGACGAATT TGTTGTGAAC GACTTCGTGG CGCACATAAA 951 TCGGCGCGCC GAACTCTTCC AAAGCACGTT CGACAATACT GATTGCCCGA 1001 TCCACACCAG CGCAGAAGCC GCGCGGATTG GCAAGGATGA TGGTTTTCTC 1051 GTTCATAAGC CCGGTATTTC GTTTTCAGAC GGCATCAATA TTTTTCTTCT 1101 TGGGTTTTAC GGTGGACGAT GTTGTCCAAC ACCGCCAACA CCGCACCGAC 1151 GCAGATAA This corresponds to the amino acid sequence <SEQ ID 2320; ORF 694.a>: a694.pep 1 LVSASGTRQK CRLKPVQTAF VLPKHSTPAS TFAQIGFGFA LAAQLFGQDE 51 HNAFFRTLAF AYGFVPPSAY GCQYFPHQHF GRGRACRYAD FVFALKPCAL 101 QVACIIHHIR IDΞARCRHFA QAVAVGRIGR TDHNHDVALF CQLFDGGLPV 151 GRRIADIFLV RIADIGETRV QRGDDVFGFI DRERGLADIG EFVGVSDFEF 201 CHISDRFDQK HFARRKLPHR SFDLDVPLMP DHDDFTVLGI QSGDFLMHFR 251 HQRASRIKHA ETALRRFLPH RLRYAVCRIN QCRARRHFRQ VFNKHRTFFT 301 QVVHDEFVVN DFVAHINRRA ELFQSTFDNT DCPIHTSAEA ARIGKDDGFL 351 VHKPGISFSD GINIFLLGFY GGRCCPTPPT PHRRR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 694 shows 100% identity over a 385 aa overlap with a predicted ORF (ORF 694) from N meningitidis: m694/a694 100.0% identity in 385 aa overlap 10 20 30 40 50 60 m694. pep LVSASGTRQKCRLKPVQTAFVLPKHΞTPASTFAQIGFGFALAAQLFGQDEHΝAFFRTLAF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a694 LVSASGTRQKCRLKPVQTAFVLPKHSTPASTFAQIGFGFALAAQLFGQDEHΝAFFRTLAF 10 20 30 40 50 60 70 80 90 100 110 120 m694.pep AYGFVPPSAYGCQYFPHQHFGRGRACRYADFVFALKPCALQVACIIHHIRIDSARCRHFA I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a694 AYGFVPPSAYGCQYFPHQHFGRGRACRYADFVFALKPCALQVACIIHHIRIDSARCRHFA 70 80 90 100 110 120 130 140 150 160 170 180 m694.pep QAVAVGRIGRTDHΝHDVALFCQLFDGGLPVGRRIADIFLVRIADIGETRVQRGDDVFGFI I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a694 QAVAVGRIGRTDHΝHDVALFCQLFDGGLPVGRRIADIFLVRIADIGETRVQRGDDVFGFI 130 140 150 160 170 180 190 200 210 220 230 240 m694.pep DRERGLADIGEFVGVSDFEFCHISDRFDQKHFARRKLPHRSFDLDVPLMPDHDDFTVLGI I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a694 DRERGLADIGEFVGVΞDFEFCHISDRFDQKHFARRKLPHRΞFDLDVPLMPDHDDFTVLGI 190 200 210 220 230 240 250 260 270 280 290 300 m694.pep QSGDFLMHFRHQRASRIKHAETALRRFLPHRLRYAVCRIΝQCRARRHFRQVFΝKHRTFFT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a694 QSGDFLMHFRHQRAΞRIKHAETALRRFLPHRLRYAVCRIΝQCRARRHFRQVFΝKHRTFFT 250 260 270 280 290 300 310 320 330 340 350 360 m694.pep QVVHDEFVVΝDFVAHIΝRRAELFQSTFDΝTDCPIHTSAEAARIGKDDGFLVHKPGISFSD I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a694 QVVHDEFVVΝDFVAHIΝRRAELFQSTFDΝTDCPIHTSAEAARIGKDDGFLVHKPGISFSD 310 320 330 340 350 360 370 380 m694.pep GIΝIFLLGFYGGRCCPTPPTPHRRRX I I I I I I I I I I I I I I I I I I I I I I I I I I a694 GIΝIFLLGFYGGRCCPTPPTPHRRRX 370 380 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2321>: g695. seq 1 TTGCCTCAAA CTCGTCCGGC AAGGCGGCAT CATCGCCATC GACAATATTT 51 TGTTGAACGG AAGGGTGATG CGCGAAGCGG CTTTTGATGC GCCGCCCAGT 101 GTCAAAATTC TCAAAGATTT CAATCAAAAC CTGCCGAACG ATACGCGGAT 151 TGTCCCCATC ACCCTGCCCG TCGGCGACGG TTTGACCCTG CTTCTGAAAA 201 AATAATGAAG ACCAAATTAC CGCTTTTTAT CATTTGGCTG TCTGTGTCTG 251 CCTCCTGTGC TTCCGTTTTA CCCGTTCCGG AGGGCAGCCG AACCGAAATG 301 CCGACACAGG AAAATGCTTC AGACGGCATT CCCTATCCCG TTCCCACTCT 351 GCAAGACCGT TTGGACTATC TGGAAGGCAA AATCGTCCGG CTGTCGAACG 401 AAGTGGAAAT GTTAAACGGG AAAGTCAAAG CATTGGAGCA TACGAAAATA 451 CACCCTTCCG GCAGGACATA CGTCCAAAAA CTCGACGACC GCAAATTGAA 501 AGAGCATTAC CTCAATACCG AAGGCGGCAG CGCATCCGCA CATACCGTCG 551 AAACCGCACA AAACCTCTAC AATCAGGCAC TCAAACACTA TCAAAACGGC 601 AGGTTTTCTG CCGCAGCCGC CTTGTTGAAG GGGGCGGACG GCGGAGACGG 651 CGGCAGCATC GCGCAACGCA GTATGTACCT GTTGCTGCAA AGCAGGGCGC 701 GTATGGGGAA CTGTGAATCT GTCATCGAAA TCGGAGGGCG TTACGCCAAC 751 CGTTTCAAAG ACAGCCCAAC CGCGCCCGAA GTCATATTCA AAATCGGCGA 801 ATGCCAATAC AGGCTTCAGC AAAAAGACAT TGCAAGGGCG ACTTGGCGCA 851 GCCTGATACA GACCTATCCC GGCAGCCCGG CGGCAAAACG CGCCGCCGCA 901 GCCGTACGCA AACGATAG This corresponds to the amino acid sequence <SEQ ID 2322; ORF 695 >: g695.pep 1 LPQTRPARRH HRHRQYFVER KGDARΞGF*C AAQCQΝSQRF QSKPAERYAD 51 CPHHPARRRR FDPASEKIMK TKLPLFIIWL SVSASCAΞVL PVPEGSRTEM 101 PTQEΝASDGI PYPVPTLQDR LDYLEGKIVR LSΝEVEMLΝG KVKALEHTKI 151 HPSGRTYVQK LDDRKLKEHY LΝTEGGSAΞA HTVETAQΝLY ΝQALKHYQΝG 201 RFSAAAALLK GADGGDGGSI AQRΞMYLLLQ SRARMGΝCES VIEIGGRYAΝ 251 RFKDSPTAPE VIFKIGECQY RLQQKDIARA TWRSLIQTYP GSPAAKRAAA 301 AVRKR* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2323>: m695.seq 1 TTGCCTCAAA CTCGTCCGTC AAGGCGGCAT CATCGCCATC GACAATATTT 51 TGCTGAACGG AAGGGTGATG CGCGAAGCGG CTTCCGATGC GCCGCCCAGC 101 GTCGGCATCC TCAAAGATTT CAATCAAAAC CTGCCGAACG ACCCGCGCAT 151 CGTCCCCATC ACCCTGCCCG TCGGCGACGG CTTGACCCTG CTTCTGAAAA 201 AATAATGAAG ATCAAATTAC CGCTTTTTAT CATTTGGCTG TCTGTGTCCG 251 CCTCCTGTGC TTCCGTTTCA CCCGTTCCGG CAGGCAGCCA AACCGAAATG 301 TCGACACGGG AAAATGCTTC AGACGGCATT CCCTATCCCG TTCCGACCTT 351 GCAAGACCGT TTGGACTATC TGGAAGGCAA AATCGTCCGG CTGTCGAACG 401 AAGTGGAAAC CTTAAACGGC AAAGTCAAAG CACTGGAACA CGCAAAAACA 451 CATTCTTCCG GCAGGGCATA CGTCCAAAAA CTCGACGACC GCAAGTTGAA 501 AGAGCATTAC CTCAATACCG AAGGCGGCAG CGCATCCGCA CATACTGTCG 551 AAACCGCACA AAACCTCTAC AATCAGGCAC TCAAACACTA TAAAAGCGGC 601 AAGTTTTCTG CCGCTGCCTC CCTGTTGAAA GGCGCGGACG GAGGCGACGG 651 CGGCAGCATC GCGCAACGCA GTATGTACCT GTTGCTGCAA AGCAGGGCGC 701 GTATGGGCAA CTGCGAATCC GTCATCGAAA TCGGAGGGCG TTACGCCAAC 751 CGTTTCAAAG ACAGCCCAAC CGCGCCTGAA GCCATGTTCA AAATCGGCGA 801 ATGCCAATAC AGGCTTCAGC AAAAAGACAT TGCAAGGGCG ACTTGGCGCA 851 GCCTGATACA GACCTATCCC GGCAGCCCGG CGGCAAAACG CGCCGCCGCA 901 GCCGTGCGCA AACGATAG This corresponds to the amino acid sequence <SEQ ID 2324; ORF 695>: m695.pep 1 LPQTRPSRRH HRHRQYFAER KGDARSGFRC AAQRRHPQRF QΞKPAERPAH 51 RPHHPARRRR LDPASEKIMK IKLPLFIIWL SVSASCASVS PVPAGSQTEM 101 STREΝASDGI PYPVPTLQDR LDYLEGKIVR LΞΝEVETLΝG KVKALEHAKT 151 HSSGRAYVQK LDDRKLKEHY LΝTEGGSASA HTVETAQΝLY ΝQALKHYKSG 201 KFSAAASLLK GADGGDGGSI AQRΞMYLLLQ ΞRARMGΝCES VIEIGGRYAΝ 251 RFKDSPTAPE AMFKIGECQY RLQQKDIARA TWRSLIQTYP GSPAAKRAAA 301 AVRKR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 694 shows 90.8% identity over a 305 aa overlap with a predicted ORF (ORF 695) from N gonorrhoeae: m695/g695 90 . 8% identity in 305 aa overlap 10 20 30 40 50 60 m695. pep PQTRPSRRHHRHRQYFAERKGDARSGFRCAAQRRHPQRFQSKPAERPAHRPHHPARRRR I I I I I I : I I I I I I I I I I : I I I I I I I I I I llll :: I g 695 PQTRPARRHHRHRQYFVERKGDARSGFXCAAQCQNSQRFQSKPAERYADCPHHPARRRR 10 20 30 40 50 60 70 80 90 100 110 120 m695.pep LDPASEKIMKIKLPLFIIWLSVSASCASVSPVPAGSQTEMSTRENAΞDGIPYPVPTLQDR :| I I I I I I I I I I I I I I I I I I I I I I I I I I III MMM I : II I I I I I I I I I I I I I I I g 695 FDPASEKIMKTKLPLFIIWLSVΞAΞCAΞVLPVPEGSRTEMPTQENASDGIPYPVPTLQDR 70 80 90 100 110 120 130 140 150 160 170 180 m695. pep LDYLEGKIVRLSNEVETLNGKVKALEHAKTHSSGRAYVQKLDDRKLKEHYLNTEGGSASA I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I : I I I I II I I I I I I I I I I I I I I I I I I g 695 LDYLEGKIVRLSNEVEMLNGKVKALEHTKIHPSGRTYVQKLDDRKLKEHYLNTEGGSASA 130 140 150 160 170 180 190 200 210 220 230 240 m695.pep HTVETAQNLYNQALKHYKSGKFSAAASLLKGADGGDGGSIAQRΞMYLLLQSRARMGNCES I I I I I I I I I I I I I I I I MM : I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g695 HTVETAQNLYNQALKHYQNGRFSAAAALLKGADGGDGGΞIAQRΞMYLLLQΞRARMGNCES 190 200 210 220 230 240 250 260 270 280 290 300 m695.pep VIEIGGRYANRFKDSPTAPEAMFKIGECQYRLQQKDIARATWRSLIQTYPGSPAAKRAAA I I II I I I I I I I I I I I I I I I MM I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g695 VIEIGGRYANRFKDSPTAPEVIFKIGECQYRLQQKDIARATWRSLIQTYPGΞPAAKRAAA 250 260 270 280 290 300 m695.pep AVRKRX llllll g695 AVRKRX The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2325>: a695.seq 1 TTGCCTCAAG CTTGTCCGGC AAGGCGGCAT CATTGCCATC GACAATATTT 51 TGTTGAACGG AAGGGTGATG CGCGAAGCGG CTTCCGATGC GCCGCCCAGC 101 GTCGGCATCC TCAAAGATTT TAATCAAAAC CTGCCGAACG ATACGCGGAT 151 TGTCCCCATC ACCCTGCCCG TCGGCGACGG TTTGACCCTG CTTCTGAAAA 201 AATAATGAAG ACCAAATTAC CGCTTTTTAT CATTTGGCTG TCCGTATCCG 251 CCGCCTGTTC TTCCCCTGTT TCCCGCAATA TTCAGGATAT GCGGCTCGAA 301 CCGCAGGCAG AGGCAGGTAG TTCGGACGCT ATTCCCTATC CCGTTCCCAC 351 TCTGCAAGAC CGTTTGGATT ATCTGGAAGG CACACTCGTC CGCCTGTCGA 401 ACGAAGTGGA AACCTTAAAC GGCAAAGTCA AAGCACTGGA GCATGCGAAA 451 ACACACCCTT CCAGCAGGGC ATACGTCCAA AAACTCGACG ACCGCAAGTT 501 GAAAGAGCAT TACCTCAATA CCGAAGGCGG CAGCGCATCC GCACATACCG 551 TCGAAACCGC ACAAAACCTC TACAATCAGG CACTCAAACA CTATAAAAGC 601 GGCAGGTTTT CTGCCGCTGC CTCCCTGTTG AAAGGCGCGG ACGGAGGCGA 651 CGGCGGCAGC ATCGCGCAAC GCAGTATGTA CCTGTTGCTG CAAAGCAGGG 701 CGCGTATGGG CAACTGCGAA TCCGTCATCG AAATCGGAGG GCGTTACGCC 751 AACCGTTTCA AAGACAGCCC AACCGCGCCT GAAGCCATGT TCAAAATCGG 801 CGAATGCCAA TACAGGCTTC AGCAAAAAGA CATTGCAAGG GCGACTTGGC 851 GCAGCCTGAT ACAGACCTAT CCCGGCAGCC CGGCGGCAAA ACGCGCCGCC 901 GCAGCCGTGC GCAAACGATA G This corresponds to the amino acid sequence <SEQ ID 2326; ORF 695. a>: 1 LPQACPARRH HCHRQYFVER KGDARSGFRC AAQRRHPQRF *SKPAERYAD 51 CPHHPARRRR FDPASEKIMK TKLPLFIIWL SVSAACSSPV SRΝIQDMRLE 101 PQAEAGSSDA IPYPVPTLQD RLDYLEGTLV RLSΝEVETLΝ GKVKALEHAK 151 THPSSRAYVQ KLDDRKLKEH YLΝTEGGΞAS AHTVETAQΝL YΝQALKHYKS 201 GRFΞAAASLL KGADGGDGGS IAQRΞMYLLL QSRARMGΝCE SVIEIGGRYA 251 ΝRFKDSPTAP EAMFKIGECQ YRLQQKDIAR ATWRSLIQTY PGSPAAKRAA 301 AAVRKR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 695 shows 88.3% identity over a 308 aa overlap with a predicted ORF (ORF 695) from N meningitidis: m695/a695 88.3% identity in 308 aa overlap 10 20 30 40 50 60 m695.pep LPQTRPSRRHHRHRQYFAERKGDARSGFRCAAQRRHPQRFQSKPAERPAHRPHHPARRRR III: M I I I I I I I I M I I I I I I I I I I I I I I I I I I I I I I I a695 LPQACPARRHHCHRQYFVERKGDARSGFRCAAQRRHPQRFXΞKPAERYADCPHHPARRRR 10 20 30 40 50 60 70 80 90 100 110 m695.pep LDPASEKIMKIKLPLFIIWLSVSASCASVSPVPAGSQT EMSTREΝASDGIPYPVPTL : I I I I I I I I I I I I I I I I I I I I I I : I : I II : I I ::: :: I I : I I I I I I I I a695 FDPAΞEKIMKTKLPLFIIWLSVΞAACSS—PVSRΝIQDMRLEPQAEAGSSDAIPYPVPTL 70 80 90 100 110 120 130 140 150 160 170 m695.pep QDRLDYLEGKIVRLΞΝEVETLΝGKVKALEHAKTHSSGRAYVQKLDDRKLKEHYLΝTEGGS M I I I I I I I I I I I I I I I I I I I I I I M I I I I I I I I I I I I I I I I I I I I I I I a695 QDRLDYLEGTLVRLSΝEVETLΝGKVKALEHAKTHPSΞRAYVQKLDDRKLKEHYLΝTEGGS 120 130 140 150 160 170 180 190 200 210 220 230 m695.pep ASAHTVETAQΝLYΝQALKHYKSGKFΞAAAΞLLKGADGGDGGΞIAQRSMYLLLQSRARMGΝ I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a695 ASAHTVETAQΝLYΝQALKHYKSGRFSAAASLLKGADGGDGGSIAQRSMYLLLQSRARMGΝ 180 190 200 210 220 230 240 250 260 270 280 290 m695.pep CESVIEIGGRYAΝRFKDSPTAPEAMFKIGECQYRLQQKDIARATWRSLIQTYPGSPAAKR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a695 CESVIEIGGRYAΝRFKDΞPTAPEAMFKIGECQYRLQQKDIARATWRSLIQTYPGSPAAKR 240 250 260 270 280 290 300 m695.pep AAAAVRKRX lllllllll a695 AAAAVRKRX 300 The following partial DΝA sequence was identified in N gonorrhoeae g696. seq: not found This corresponds to the amino acid sequence < ORF 696.ng>: g696.ρep: not found The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2327>: m696.seq 1 TTGGGTTGCC GGCAGGCGGC ATCCCATCAT TTTTGCCAAG GCAACAAATT 51 ATTTGGCGGC ATCTTTCATT TTGTCTGCCG CTTCCTGAGT CGCGTCGGCA 101 GCTTTGTTCA AAGTATCTTT AGCTGCTTCA GTTACAGCTT CTTTGGCTTC 151 AGTTACAGCT TCCTCGGCAC TTGCCTTTGC ATCAGCCGCA GCATCTTTGA 201 CTTGGTCTTT CGCTTCTTCG ACGGCAGAAG CGGCAGACTC GGCGGCAGAA 251 GCCGCAGTGT CTTTAACATC GGACTCAACG GCTTGAACCG CTTCCTTAAC 301 CTCCTGTTTG GCTTCTTGCG AACAAGCTGC CAAGGCAGCC GCCATCATTG 351 CGGCAATCAA TAA This corresponds to the amino acid sequence <SEQ ID 2328; ORF 696>: m696.pep 1 LGCRQAASHH FCQGΝKLFGG IFHFVCRFLS RVGSFVQΞIF SCFΞYΞFFGF 51 SYSFLGTCLC ISRSIFDLVF RFFDGRSGRL GGRSRSVFΝI GLΝGLΝRFLΝ 101 LLFGFLRTΞC QGΞRHHCGΝQ * The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2329>: a696. seq 1 TTGGGTTGCC GGCAGGCGGC ATCCCATCAT TTTTGCCAAG GCAACAAATT 51 ATTTGGCGGC ATCTTTCATT TTGTCTGCCG CTTCCTGAGT CGCGTCGGCA 101 GCTTTGTTCA AAGTATCTTT AGCTGCTTCA GTTACAGCTT CTTTGGCTTC 151 AGTTACAGCT TCCTCGGCAC TTGCCTTTGC ATCAGCCGCA GCATCTTTGA 201 CTTGGTCTTT CGCTTCTTCG ACGGCAGAAG CGGCAGACTC GGCGGCAGAA 251 GCCGCAGTGT CTTTAACATC GGACTCAACG GCTTGAACCG CTTCCTTAAC 301 CTCCTGTTTG GCTTCTTGCG AACAAGCTGC CAAGGCAGCC GCCATCATTG 351 CGGCAATCAA TAA This corresponds to the amino acid sequence <SEQ ID 2330; ORF 696. a>: a696.pep 1 LGCRQAASHH FCQGNKLFGG IFHFVCRFLS RVGSFVQSIF SCFΞYSFFGF 51 SYΞFLGTCLC ISRSIFDLVF RFFDGRSGRL GGRSRSVFNI GLNGLNRFLN 101 LLFGFLRTSC QGSRHHCGNQ * Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 696 shows 100.0% identity over a 120 aa overlap with a predicted ORF (ORF 696. a) from N meningitidis: 696/a696 100.0% identity in 120 aa overlap 10 20 30 40 50 60 m696.pep LGCRQAASHHFCQGΝKLFGGIFHFVCRFLΞRVGΞFVQSIFSCFSYSFFGFSYSFLGTCLC I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I a 696 LGCRQAASHHFCQGΝKLFGGIFHFVCRFLSRVGSFVQSIFSCFSYΞFFGFSYSFLGTCLC 10 20 30 40 50 60 70 80 90 100 110 120 m696.pep ISRΞIFDLVFRFFDGRSGRLGGRSRSVFΝIGLΝGLΝRFLΝLLFGFLRTSCQGSRHHCGΝQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I a 696 ISRSIFDLVFRFFDGRSGRLGGRSRSVFΝIGLΝGLΝRFLΝLLFGFLRTSCQGSRHHCGΝQ 70 80 90 100 110 120 m696.pep X I a696 X The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 2331>: 1 ATGAGCAGCC TGATGACGTT GTTTTCGGTA TTGGTACCGA TGTTTGCCGG 51 ATTTTTTATC CGTGTTCCCA AGCCTTACCT GCCCGCTTCG GACAAGGTGC 101 TGTCGGTTTT GGTGTATGCC GTGCTGCTGC TGATCGGCGT ATCGTTGTCG 151 CGCGTGGAGG ATTTGGGTTC GCGGTTGGGC GATATGGCGT TGACGGTTCT 201 GTGGCTGTTT GTTTGTACGG TAGGGGCGAA CCTGCTTGCC TTGGCAGTGT 251 TGGGAAAGTT GTCCCCGTGG CGGATAGGGG GAAAAGGGAA GGGCGTTTCG 301 GTCGGCGTGT CGGGCAGTGT GAGGCAGCTC GGATGCGTAC TGCTCGGTTT 351 TGTGTCCGGC AAATTGATGT GCGATATTTG GATGCCGTCT GAAAACGCGG 401 GTATGTACTG CCTGATGCTG CTGGTGTTCC TCATCGGCGT ACAGCTCAAA 451 AGTAGCGGCG TATCGTTGCG GCAGGTTTTG CTTAACCGGC GGGGCATCCG 501 GCTGTCGGTT TGGTTTATAT TGTCATCTCT TTCAGGCGGG CTGCTGTTTG 551 CCGCATCGGC AGATGGTGTG TCGTGGACGA AAGGTTTGGC GATGGCTTCC 601 GGCTTCGGTT GGTATTCCCT CTCGGGTTTG GTAATGACCG AGGCTTACGG 651 GGCGGTATGG GGCAGCATCA TGCTGCTGAA CGATTTGGCA CGAGAGCTGT 701 TTGCACTGGC ATTTATTCCG CTGCTGATGA AGCGTTTTCC GGATGCGGCG 751 GTGGGGGTCG GCGGCGCGAC CAGTATGGAT TTCACATTGC CCGTAATTCA 801 GGGTGCGGGC GGTTTGGAAG TCGTGCCGGT AGCGGTCAGC TTCGGCGTGG 851 TGGTCAATAT CGCCGCCCCG TTTCTGATGG TGGTGTTTTC CACGCTGGGC 901 TGA This corresponds to the amino acid sequence <SEQ LD 2332; ORF 700>: g700.pep 1 MSSLMTLFSV LVPMFAGFFI RVPKPYLPAS DKVLSVLVYA VLLLIGVSLS 51 RVEDLGSRLG DMALTVLWLF VCTVGAΝLLA LAVLGKLSPW RIGGKGKGVS 101 VGVSGSVRQL GCVLLGFVSG KLMCDIWMPS EΝAGMYCLML LVFLIGVQLK 151 SSGVSLRQVL LΝRRGIRLSV WFILSSLSGG LLFAASADGV SWTKGLAMAS 201 GFGWYSLSGL VMTEAYGAVW GSIMLLNDLA RELFALAFIP LLMKRFPDAA 251 VGVGGATSMD FTLPVIQGAG GLEWPVAVS FGWVNIAAP FLMWFSTLG 301 * The following partial DNA sequence was identified in N meningitidis <SEQ ID 2333>: m700.seq 1 ATGGACAGCC TGATGACGTT GCTTTCGGTA TTGATACCGA TGTTTGCCGG 51 ATTTTTTATC CGTGTGCCCA AGCCTTACCT GCCCGCTTTG GATAAGGTGC 101 TATCGGTCTT GGTGTATGCT GTGCTGCTGC TGATCGGCGT CTCGTTGTCG 151 CGCGTGGAGG ATTTGGGTTC GCGGTTGGAC GATATGGCGT TGACGGTTCT 201 GTGGCTGTTT GTTTGTACGG TCGGGGCGAA CCTGCTTGCT TTGGCAGTGT 251 TGGGAAAGTT ATTCCCGTGG CGGATAAAGG GGAAAGGGAA GGGCGTTTCG 301 GTCGGCGTGT CGGGCAGTGT GGGGCAGCTC GGATGCGTGC TGCTCGGATT 351 TGCATTCGGC AAACTGATGC GCGATATTTG GATGCCGTCT GAAAGCGCGG 401 GCATGTATTG TCTGATGCTG CTGGTGTTCC TCATCGGCGT ACAGCTCAAA 451 AGCAGCGGCG TATCGTTGCG GCAGGTTTTG GTCAACCGCA GGGGTATTCG 501 GTTGTCGGTC TGGTTTATGC TTTCATCTCT TTCGGGCGGG CTGCTGTTTG 551 CCGCATCGAC AGACGGTGTG TCGTGGACGA AAGGTTTGGC GATGGCTTCC 601 GGCTTCGGTT GGTATTCCCT CTCGGGTTTG GTCATGACCG AGGCTTACGG 651 CGCGGTATGG GGCAGCATCA TGCTGCTGAA CGATTTGGCA CGAGAGCTGT 701 TTGCACTGGC ATTTATCCCG CTGCTGATGA AGCGTTTTCC AGATGCGGCG 751 GTGGGGGTTG GCGGTGCGAC CAGTATGGAT TTTACATTGC CCGTGATTCA 801 GGGTGCGGGC GGTTTGGAAG TCGTGCCGGT AGCGGTCAGC TTCGGCGTGG 851 TGGTCAATAT CGCCGCCCCG TTTCTGATGG TGGTGTTTTC CGCTTTGGGT 901 TGA This corresponds to the amino acid sequence <SEQ ID 2334; ORF 700>: 1 MDSLMTLLSV LIPMFAGFFI RVPKPYLPAL DKVLSVLVYA VLLLIGVSLS 51 RVEDLGSRLD DMALTVLWLF VCTVGAΝLLA LAVLGKLFPW RIKGKGKGVS 101 VGVSGSVGQL GCVLLGFAFG KLMRDIWMPS ESAGMYCLML LVFLIGVQLK 151 SSGVSLRQVL VΝRRGIRLSV WFMLSSLSGG LLFAASTDGV SWTKGLAMAS 201 GFGWYSLSGL VMTEAYGAVW GSIMLLNDLA RELFALAFIP LLMKRFPDAA 251 VGVGGATSMD FTLPVIQGAG GLEWPVAVS FGWVNIAAP FLMWFSALG 301 * Computer analysis of the amino acid sequences gave the following results: Homology with a predicted ORF from N. gonorrhoeae with menB ORF 700 shows 94.7% identity over a 300 aa overlap with a predicted ORF (ORF700.ng) from N gonorrhoeae: m700/g700 10 20 30 40 50 60 m700.pep MDSLMTLLSVLIPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLLIGVSLSRVEDLGSRLD I M I II I M I M II II II II II II I II II MMMMMMMMMMMIMMM g700 MSSLMTLFSVLVPMFAGFFIRVPKPYLPASDKVLSVLVYAVLLLIGVSLSRVEDLGSRLG 10 20 30 40 50 60 70 80 90 100 110 120 m700. pep DMALTVLWLFVCTVGAΝLLALAVLGKLFPWRIKGKGKGVSVGVSGSVGQLGCVLLGFAFG I I II II II I II II I II II I II II II II I II I II II II I II II I II II II I I II M I g700 DMALTVLWLFVCTVGAΝLLALAVLGKLSPWRIGGKGKGVSVGVSGSVRQLGCVLLGFVSG 70 80 90 100 110 120 130 140 150 160 170 180 m700.pep KLMRDIWMPSESAGMYCLMLLVFLIGVQLKSSGVSLRQVLVΝRRGIRLSVWFMLSSLSGG III II II II I : II II II II II II II II II II II II II II M II I II II I II : II I II II g700 KLMCDIWMPSEΝAGMYCLMLLVFLIGVQLKSSGVSLRQVLLΝRRGIRLSVWFILSSLSGG 130 140 150 160 170 180 190 200 210 220 230 240 m700.pep LLFAASTDGVSWTKGLAMASGFGWYSLSGLVMTEAYGAVWGSIMLLΝDLARELFALAFIP I II II I : I II II II I II I II II I II II I II I I I I II II II II I I I II I II II I II II II I g700 LLFAASADGVSWTKGLAMASGFGWYSLSGLVMTEAYGAVWGSIMLLΝDLARELFALAFIP 190 200 210 220 230 240 250 260 270 280 290 300 m700 . pep LLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEWPVAVSFGVWNIAAPFLMWFSALG M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M g700 LLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEVVPVAVSFGVWNIAAPFLMVVFSTLG 250 260 270 280 290 300 m700 . pep X I g700 X The following partial DNA sequence was identified in N meningitidis <SEQ ID 2335>: a700.seq 1 ATGGACAGCC TGATGACGTT GCTTTCGGTA TTGATACCGA TGTTTGCCGG 51 ATTTTTTATC CGTGTGCCCA AGCCTTACCT GCCCGCTTTG GATAAGGTGC 101 TATCGGTCTT GGTGTATGCT GTGCTGCTGC TGATCGGCGT CTCGTTGTCG 151 CGCGTGGAGG ATTTGGGTTC GCGGTTGGAC GATATGGCGT TGACGGTTCT 201 GTGGCTGTTT GTTTGTACGG TCGGGGCGAA CCTGCTTGCT TTGGCAGTGT 251 TGGGAAAGTT ATTCCCGTGG CGGATAAAGG GGAAAGGGAA GGGCGTTTCG 301 GTCGGTGTGT CGGGCAGTGT GGGGCAGCTC GGATGCGTGC TGCTCGGATT 351 TGCATCCGGC AAACTGATGC GCGATATTTG GATGCCGTCT GAAAACGCGG 401 GTATGTATTG TCTGATGCTG CTGGTGCTCΝ TCATCGGCGT ACAGCTCAAA 451 AGCAGCGGCG TATCGTTGCG GCAGGTTTTG GTCAACCGCA GGGGTATTCG 501 GTTGTCGGTC TGGTTTATGC TTTCATCTCT TTCAGGCGGG CTGCTGTTTG 551 CCGCATCGGC AGACGGTGTG TCGTGGGTGA AAGGTTTGGC GATGGCTTCC 601 GGCTTCGGTT GGTATTCCCT CTCGGGTTTG GTGATGACCG AGGCTTACGG 651 CGCGGTATGG GGCAGTATCG CGCTTTTGAA CGATTTGGCA CGAGAGCTGT 701 TCGCGCTGGC ATTTATTCCG CTGCTGATGA AGCGTTTTCC CGATGCGGCA 751 GTGGGGGTCG GCGGCGCGAC CAGTATGGAT TTCACATTGC CCGTGATTCG 801 GGGTGCGGGC GGCTTGGAAG CCGTACCGGT AGCGGTCAGC TTCGGCGTGG 851 TGGTCAATAT CGCCGCTCCG TTTCTGATGG TGGTGTTTTC CGCTTTGGGC 901 TGA This corresponds to the amino acid sequence <SEQ ID 2336; ORF 700.a>: a700.pep 1 MDSLMTLLSV LIPMFAGFFI RVPKPYLPAL DKVLSVLVYA VLLLIGVSLS 51 RVEDLGSRLD DMALTVLWLF VCTVGAΝLLA LAVLGKLFPW RIKGKGKGVS 101 VGVSGSVGQL GCVLLGFASG KLMRDIWMPS EΝAGMYCLML LVLXIGVQLK 151 SSGVSLRQVL VΝRRGIRLSV WFMLSSLSGG LLFAASADGV SWVKGLAMAS 201 GFGWYSLSGL VMTEAYGAVW GSIALLΝDLA RELFALAFIP LLMKRFPDAA 251 VGVGGATSMD FTLPVIRGAG GLEAVPVAVS FGWVNIAAP FLMWFSALG 301 * m700/a700 97.0% identity in 300 aa overlap 10 20 30 40 50 60 m700 • pep MDSLMTLLSVLIPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLLIGVSLSRVEDLGSRLD I II II II II I II II I II I II II II II I II II II II II II II II II II II I II II II II II a700 MDSLMTLLSVLIPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLLIGVSLSRVEDLGSRLD 10 20 30 40 50 60 70 80 90 100 110 120 m700.pep DMALTVLWLFVCTVGANLLALAVLGKLFPWRIKGKGKGVSVGVSGSVGQLGCVLLGFAFG II I II II II II II II II I I II II II I II II I I II I II I II II II II II I II II II II I I a700 DMALTVLWLFVCTVGANLLALAVLGKLFPWRIKGKGKGVΞVGVSGSVGQLGCVLLGFASG 70 80 90 100 110 120 130 140 150 160 170 180 m700.pep KLMRDIWMPSESAGMYCLMLLVFLIGVQLKSSGVSLRQVLVNRRGIRLSVWFMLSSLSGG II II II II I I M II I II I II I M II II II I I II I II II II II II I II I II II II II II I a700 KLMRDIWMPSENAGMYCLMLLVLXIGVQLKSSGVSLRQVLVNRRGIRLSVWFMLSSLSGG 130 140 150 160 170 180 190 200 210 220 230 240 m700. pep LLFAASTDGVSWTKGLAMASGFGWYSLSGLVMTEAYGAVWGSIMLLNDLARELFALAFIP I I II II : I II II : II I II II II II I II II II II I II II II II I II II I I II I I I I I I I I a700 LLFAASADGVSWVKGLAMASGFGWYSLSGLVMTEAYGAVWGSIALLNDLARELFALAFIP 190 200 210 220 230 240 250 260 270 280 290 300 m700.pep LLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEVVPVAVSFGVWNIAAPFLMVVFSALG II II I II I I II I II II II II II I II I : II I II I : I II II II II II II II I II II II II II a700 LLMKRFPDAAVGVGGATSMDFTLPVIRGAGGLEAVPVAVSFGVWNIAAPFLMWFSALG 250 260 270 280 290 300 m700.pep X I a700 X The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 2337>: g701.seq 1 ATGTCTTGGC ACATATTCCA AGTTGCAGGG ATACCGACCG CTTCGATGGC 51 ACAATCTACG CCGTCTTCGC CGACGATGGC GAAAACTTGT TTGGAGACGT 101 CGCCGGAAGC GGGGCTGATG GTATGGGTCG CGCCCAACTC TTTCGCCGGT 151 TTCAAACGGT TTTCGTCCAT ATCGCACACG ATAATGGCGG CAGGGCTATA 201 CAGTTGGGCG GTCAACAAGG CGGACATACC GACAGGGCCG GCACCTGCGA 251 TGAATACGGT ATCGCCGGGT TTCACATCGC CGTATTGCAC GCCGATTTCG 301 TGGGCGGTCG GTAAAGCGTC GCTCAACAGC AGGGCGATTT CTTCGTTGAC 351 GTTGTCGTGC GGCGGCACGA GGCTGTTGTC GGCATAA This corresponds to the amino acid sequence <SEQ ID 2338; ORF 701>: g701.pep 1 MSWHIFQVAG IPTASMAQST PSSPTMAKTC LETSPEAGLM VWVAPΝSFAG 51 FKRFSSISHT IMAAGLYSWA VΝKADIPTGP APAMΝTVSPG FTSPYCTPIS 101 WAVGKASLΝS RAISSLTLSC GGTRLLSA* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2339>: m701.seq 1 ATGTCTTGGC ACATATTCCA TGTAGCAGGG ATACCGACGG CTTCGATGGC 51 GCAATCCACG CCGTCTTCGC CGACGATGGC AAAGACTTGT TTGGATACTT 101 CGCCGGAAGC AGGGTTAATG GTATGGGTCG CACCCAATTC TTTCGCCAGT 151 TTCAAACGGT TTTCGTCCAT ATCGCAAACG ATGATGGCGG CGGGACTGTA 201 CAGTTGGGCG GTCAACAGGG CGGACATACC GACAGGGCCT GCCCCAGCGA 251 TGAATACGGT GTCGCCGGGT TTGACATCGC CGTATTGCAC GCCGATTTCG 301 TGGGCGGTCG GCAAAGCGTC GCTCAACAAC AGGGCGATTT CTTCGTTGAC 351 ATTATCGGGC AGCGGAACGA GGCTGTTGTC GGCATAA This corresponds to the amino acid sequence <SEQ ID 2340; ORF 701>: m701.pep 1 MSWHIFHVAG IPTASMAQST PSSPTMAKTC LDTSPEAGLM VWVAPΝSFAS 51 FKRFΞSISQT MMAAGLYSWA VΝRADIPTGP APAMΝTVSPG LTSPYCTPIS 101 WAVGKASLΝΝ RAISSLTLSG SGTRLLSA* Computer analysis of the amino acid sequences gave the following results: Homology with a predicted ORF from N gonorrhoeae with menB ORF 701 shows 92.2% identity over a 128 aa overlap with a predicted ORF (ORF701.ng) from N gonorrhoeae: m701/g701 10 20 30 40 50 60 m701.pep MSWHIFHVAGIPTASMAQSTPSSPTMAKTCLDTSPEAGLMVWVAPΝSFASFKRFSSISQT M II I M II I I I II II II II II II II II I IMM II II I I I II II II I I: II I II II M I g701 MSWHIF Q VAGIPTASMAQSTPSSPTMAKTCLETSPEAGLMVWVAPΝSFAGFKRFSSISHT 10 20 30 40 50 60 70 80 90 100 110 120 m701.pep MMAAGLYSWAVNRADIPTGPAPAMNTVSPGLTSPYCTPISWAVGKASLNNRAISSLTLSG : II I II II II II M I I II II I I II I II II M II I I II II II I II II II I : I I II I I I II g701 IMAAGLYSWAVNKADIPTGPAPAMNTVSPGFTSPYCTPISWAVGKASLNSRAISSLTLSC 70 80 90 100 110 120 129 m701.pep SGTRLLSAX : I I II II II g701 GGTRLLSAX The following partial DNA sequence was identified in N meningitidis <SEQ ID 2341>: a701.seq 1 ATGTCTTGGC ACATATTCCA AGTTGCAGGG ATACCGACGG CTTCGATCGC 51 GCAGTCCACG CCGTCTTCGC CGACGATAGC GGCAACTTGC TTGCTTACAT 101 CGCCGGAAGC AGGGTTAATG GTATGGGTTG CGCCCAACTC TTTCGCCAGT 151 TTCAAACGGT TTTCGTCCAT ATCGCAAACA ATGATGGCGG CGGGGCTGTA 201 CAGTTGGGCG GTCGGCAAGG CGGACATACC GACAGGAGCG GCACCTGCGA 251 TGAATACGGT GTCGCCGGGT TTGACATCGC CGTATTGCAC GCCGATTTCG 301 TGTGCGGTCG GCAAAGCGTC GCTCAACAAC AGGGCGACTT CTTCGTTGAC 351 GTTGTCGGGC AGCGGCACGA GGCTGTTGTC GGCATAA This corresponds to the amino acid sequence <SEQ ID 2342; ORF 701.a>: a701.pep 1 MSWHIFQVAG IPTASIAQST PSSPTIAATC LLTSPEAGLM VWVAPΝSFAS 51 FKRFSSISQT MMAAGLYSWA VGKADIPTGA APAMΝTVSPG LTSPYCTPIS 101 CAVGKASLΝΝ RATSSLTLSG SGTRLLSA* m701/a701 92.2% identity in 128 aa overlap 10 20 30 40 50 60 m701.pep MSWHIFHVAGIPTASMAQSTPSSPTMAKTCLDTSPEAGLMVWVAPΝSFASFKRFSSISQT II II I M II II II I M II II II II I M III II II II II II II I I I I I I I II I II II II a701 MSWHIFQVAGIPTASIAQSTPSSPTIAATCLLTSPEAGLMVWVAPΝSFASFKRFSSISQT 10 20 30 40 50 60 70 80 90 100 110 120 m701.pep MMAAGLYSWAVΝRADIPTGPAPAMΝTVSPGLTSPYCTPISWAVGKASLΝΝRAISSLTLSG II II II I II II :: I II II I II II I I I I I I II I II I I II I II I I II I I II I lllllll a701 MMAAGLYSWAVGKADIPTGAAPAMΝTVSPGLTSPYCTPISCAVGKASLΝΝRATSSLTLSG 70 80 90 100 110 120 129 m701.pep SGTRLLSAX I II II I II I a701 SGTRLLSAX The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 2343>: g702. seq 1 ATGCCGTGTt ccaAAGCCAG TTGGACTTCG CCCGGAGtgg cAACGCCGGG 51 AATCAGGGGA ATGCCGCTGT TGCGGCCGGC TCTGGCGAGG GATTCGTGCA 101 AACCCGGGCT GATGGCGAAA ACCGCGCCTG CGTCTTCGAC GGCTTTGAGC 151 TGTTCGGGAT TGGTTACCGT ACCTGCGCCG ATGATGGCGT TGGGCATTTC 201 TTTGGCAATC AGGCGGATGG CCTCGAGTCC GACGGGGGTG CGCAAGGTAA 251 TTTCGAGGGT GGGGATGCCG CCTTCGACAA GGGCGCGGGA CAAATCGACG 301 GCGGTGCTTA AGTCGTCAAt cgCCATCACA GGCACAACTG CGCCGGCGGT 351 CAGGATTTCG cggggggtca gttga This corresponds to the amino acid sequence <SEQ ID 2344; ORF 702>: g702.pep 1 MPCSKASWTS PGVATPGIRG MPLLRPALAR DSCKPGLMAK TAPASSTALS 51 CSGLVTVPAP MMALGISLAI RRMASSPTGV RKVISRVGMP PSTRARDKST 101 AVLKSSIAIT GTTAPAVRIS RGVS* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2345>: m702.seq 1 ATGCCGTGTT CCAAAGCCAG TTGGATTTCG CCCGGGGTGG CAACACCGGG 51 AATCAGGGGG ATGCCGCTGT TGTGGCCGGC TTTGGCGAGG GATTCATGCA 101 GCCCCGGGCT GATGGCGAAA ACCGCGCCTG CGTCTTCGAC GGCTTTGAGC 151 TGTTCGGGAT TGGTTACCGT ACCTGCGCCG ACGATGGCGT TGGGCACTTC 201 TTTGGCAATC AGGCGGATGG CATCGAGGCC GACAGGGGTG CGCAGGGTGA 251 TTTCGAGGGT AGGGATGCCG CCTTCGACAA GGGCGTGGGA CAAATCGATG 301 GCGGTGCTTA AGTCGTCAAT CGCCATTACC GGCACAACTG CGCCGGCGGT 351 CAAAATTTCG CGGGGGGTCA GTTTGGACAT TTCGGTTCTC CGGGTGGAAT 401 GGGGTATTTT ATTAAGATGG GACAGGTTGT AG This corresponds to the amino acid sequence <SEQ ID 2346; ORF 702>: m702.pep 1 MPCSKASWIS PGVATPGIRG MPLLWPALAR DSCSPGLMAK TAPASSTALS 51 CSGLVTVPAP TMALGTSLAI RRMASRPTGV RRVISRVGMP PSTRAWDKSM 101 AVLKSSIAIT GTTAPAVKIS RGVSLDISVL RVEWGILLRW DRL* ORF 702 shows 91.9% identity over a 124 aa overlap with a predicted ORF (ORF702.ng) from N gonorrhoeae: m702/g702 10 20 30 40 50 60 m702. pep MPCSKASWISPGVATPGIRGMPLLWPALARDSCSPGLMAKTAPASSTALSCSGLVTVPAP llllllll MMMMIMMM II II II II : II II II II II II II II II II II II II g702 MPCSKASWTSPGVATPGIRGMPLLRPALARDSCKPGLMAKTAPASSTALSCSGLVTVPAP 10 20 30 40 50 60 70 80 90 100 110 120 m702.pep TMALGTSLAIRRMASRPTGVRRVISRVGMPPSTRAWDKSMAVLKSSIAITGTTAPAVKIS II II II II II II I I II IM II II II II II II I III I I I II II II II II I I I M II g702 MMALGISLAIRRMASSPTGVRKVISRVGMPPSTRARDKSTAVLKSSIAITGTTAPAVRIS 70 80 90 100 110 120 130 140 m702.pep RGVSLDISVLRVEWGILLRWDRLX llll g702 RGVSX The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2347>: a702. seq 1 ATGCCGTGTT CCAAAGCCAG TTGGATTTCG CCCGGGGTGG CAACACCGGG 51 AATCAGGGGG ATGCCGCTGT TGTGGCCGGC TTTGGCGAGG GATTCATGCA 101 GCCCCGGGCT GATGGCGAAA ACCGCGCCTG CGTCTTCGAC GGCTTTGAGC 151 TGTTCGGGAT TGGTTACCGT ACCTGCGCCG ACGATGGCGT TGGGCACTTC 201 TTTGGCAATC AGGCGGATGG CATCGAGGCC GACAGGGGTG CGCAGGGTGA 251 TTTCGAGGGT AGGGATGCCG CCTTCGACAA GGGCGTGGGA CAAATCGATG 301 GCGGTGCTTA AGTCGTCAAT CGCCATTACC GGCACAACTG CGCCGGCGGT 351 CAAAATTTCG CGGGGGGTCA GTTTGGACAT TTCGGTTCTC CGGGTGGAAT 401 GGGGTATTTT ATTAAGATGG GACAGGTTGT AG This corresponds to the amino acid sequence <SEQ ID 2348; ORF 702.a>: a702.pep 1 MPCSKASWIS PGVATPGIRG MPLLWPALAR DSCSPGLMAK TAPASSTALS 51 CSGLVTVPAP TMALGTSLAI RRMASRPTGV RRVISRVGMP PSTRAWDKSM 101 AVLKSSIAIT GTTAPAVKIS RGVSLDISVL RVEWGILLRW DRL* m702/a702 100.0% identity in 143 aa overlap 10 20 30 40 50 60 m702.pep MPCSKASWISPGVATPGIRGMPLLWPALARDSCSPGLMAKTAPASSTALSCSGLVTVPAP MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a702 MPCSKASWISPGVATPGIRGMPLLWPALARDSCSPGLMAKTAPASSTALSCSGLVTVPAP 10 20 30 40 50 60 70 80 90 100 110 120 m702. pep TMALGTSLAIRRMASRPTGVRRVISRVGMPPSTRAWDKSMAVLKSSIAITGTTAPAVKIS MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a702 TMALGTSLAIRRMASRPTGVRRVISRVGMPPSTRAWDKSMAVLKSSIAITGTTAPAVKIS 70 80 90 100 110 120 130 140 m702.pep RGVSLDISVLRVEWGILLRWDRLX I I II II II I II II I II II II I II I a702 RGVSLDISVLRVEWGILLRWDRLX 130 140 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2349>: g703. seq 1 ATGAAAGCAA AAATCCTGAC TTCCGTTGCG CTGCTTGCCT GTTCCGGCAG 51 CCTGTTTGCC CAAACGCTGG CAACCGTTAA CGGTCAGAAA ATCGACAGTT 101 CCGTCATCGA TGCGCAGGTT GCCGCATTCC GTGCGGAAAA CAGCCGTGCC 151 GAAGACACGC CGCAACTGCG CCAATCCCTG CTGGAAAACG AAGTGGTCAA 201 CACCGTGGTC GCACAGGAAG TGAAACGCCT GAAACTCGAC CGGTCGGCAG 251 AGTTTAAAGA TGCGCTTGCC AAATTGCGTG CCGAAGCGAA AAAGTCGGGC 301 GACGACAAGA AACCGTCCTT CAAAACCGTT TGGCAGGCGG TAAAATATGG 351 CTTGAACGGC GAGGCATACG CACTGCATAT CGCCAAAACC CAACCGGTTT 401 CCGAGCAGGA AGTAAAAGCC GTTTACGACA ATATCAGCGG TTTTTATAAA 451 GGCACGCAGG AAGTCCAGTT GGGCGAAATC CTGACCGACA AGGAAGAAAA 501 TGCGAAAAAA GCGGTTGCCG ATTTGAAGGC GAAAAAAGGT TTTGATGCCG 551 TTTTGAAACA ATACTCGCTC AACGACCGCA CCAAACGGAC CGGCGCGCCG 601 GACGGATATG TGCCGCTGAA AGATTTGGAA CAGGGTGTTC CGCCGCTTTA 651 TCAGGCAATT AAGGACTTGA AAAAAGGCGA ATTTACGGCA ACGCCGCTGA 701 AAAACGGCGA TTTCTACGGC GTTTATTATG TCAACGACAG CCGcgaggTG 751 AAAGTGCCTT CTTTTGACGA AATGAAAGGA CAGATTGCCG GCAACCTTCA 801 GGCGGAACGG ATTGACCGTG CCGTctgTGc gcTGTTgggt aaggCAAACA 851 TCAAACCTGC AAAATAA This corresponds to the amino acid sequence <SEQ ID 2350; ORF 703>: g703.pep 1 MKAKILTSVA LLACSGSLFA QTLATVΝGQK IDSSVIDAQV AAFRAEΝSRA 51 EDTPQLRQSL LEΝEWΝTW AQEVKRLKLD RSAEFKDALA KLRAEAKKSG 101 DDKKPSFKTV WQAVKYGLΝG EAYALHIAKT QPVSEQEVKA VYDΝISGFYK 151 GTQEVQLGEI LTDKEEΝAKK AVADLKAKKG FDAVLKQYSL ΝDRTKRTGAP 201 DGYVPLKDLE QGVPPLYQAI KDLKKGEFTA TPLKΝGDFYG VYYVΝDSREV 251 KVPSFDEMKG QIAGΝLQAER IDRAVCALLG KAΝIKPAK* The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 2351>: m703.seq 1 ATGAAAGCAA AAATCCTGAC TTCCGTTGCA CTGCTTGCCT GTTCCGGCAG 51 CCTGTTTGCC CAAACGCTGG CAACCGTCAA CGGTCAGAAA ATCGACAGTT 101 CCGTCATCGA TGCGCAGGTT GCCGCATTCC GTGCGGAAAA CAGCCGTGCC 151 GAAGACACGC CGCAACTGCG CCAATCCCTG CTGGAAAACG AAGTGGTCAA 201 TACCGTGGTC GCACAGGAAG TGAAACGCCT GAAACTCGAC CGGTCGGCAG 251 AGTTTAAAAA TGCGCTTGCC AAATTGCGTG CCGAAGCGAA AAAGTCGGGC 301 GACGACAAGA AACCGTCCTT CAAAACCGTT TGGCAGGCGG TAAAATATGG 351 CTTGAACGGC GAGGCATACG CATTGCATAT CGCCAAAACC CAACCGGTTT 401 CCGAGCAGGA AGTAAAAGCC GCATATGACA ATATCAGCGG TTTTTACAAA 451 GGTACGCAGG AAGTCCAGTT GGGCGAAATC CTGACCGACA AGGAAGAAAA 501 TGCAAAAAAA GCGGTTGCCG ACTTGAAGGC GAAAAAAGGT TTCGATGCCG 551 TCTTGAAACA ATATTCCCTC AACGACCGTA CCAAACAGAC CGGTGCGCCG 601 GTCGGATATG TGCCGCTGAA AGATTTGGAA CAGGGTGTTC CGCCGCTTTA 651 TCAGGCAATT AAGGACTTGA AAAAAGGCGA ATTTACGGCA ACGCCGCTGA 701 AAAACGGCGA TTTCTACGGC GTTTATTATG TCAACGACAG CCGCGAGGTA 751 AAAGTGCCTT CTTTTGATGA AATGAAAGGA CAGATTGCGG GCAACCTTCA 801 GGCGGAACGG ATTGACCGTG CCGTCGGTGC ACTGTTGGGC AAGGCAAACA 851 TCAAACCTGC AAAATAA This corresponds to the amino acid sequence <SEQ ID 2352; ORF 703>: m703.pep 1 MKAKILTSVA LLACSGSLFA QTLATVNGQK IDSSVIDAQV AAFRAENSRA 51 EDTPQLRQSL LENEWNTW AQEVKRLKLD RSAEFKNALA KLRAEAKKSG 101 DDKKPSFKTV WQAVKYGLNG EAYALHIAKT QPVSEQEVKA AYDNISGFYK 151 GTQEVQLGEI LTDKEENAKK AVADLKAKKG FDAVLKQYSL NDRTKQTGAP 201 VGYVPLKDLE QGVPPLYQAI KDLKKGEFTA TPLKNGDFYG VYYVNDSREV 251 KVPSFDEMKG QIAGNLQAER IDRAVGALLG KANIKPAK* ORF 703 shows 98.3% identity over a 288 aa overlap with a predicted ORF (ORF703.ng) from N gonorrhoeae: m703/g703 10 20 30 40 50 60 m703.pep MKAKILTSVALLACSGSLFAQTLATVΝGQKIDSSVIDAQVAAFRAEΝSRAEDTPQLRQSL II I II II II II II II II II II I II II II I II II II I II II II I II II II II II II I II I I g703 MKAKILTSVALLACSGSLFAQTLATVΝGQKIDSSVIDAQVAAFRAEΝSRAEDTPQLRQSL 10 20 30 40 50 60 70 80 90 100 110 120 m703.pep LEΝEWΝTWAQEVKRLKLDRSAEFKΝALAKLRAEAKKSGDDKKPSFKTVWQAVKYGLΝG II II II II II I II I II II I II II I II M II I II I I II II II II II I II II II II II I II I g703 LEΝEWΝTWAQEVKRLKLDRSAEFKDALAKLRAEAKKSGDDKKPSFKTVWQAVKYGLΝG 70 80 90 100 110 120 130 140 150 160 170 180 m703.pep EAYALHIAKTQPVSEQEVKAAYDΝISGFYKGTQEVQLGEILTDKEEΝAKKAVADLKAKKG I II II II II II II I II II II M II II II II II II I II II II I I II II II II II I II II II g703 EAYALHIAKTQPVSEQEVKAVYDΝISGFYKGTQEVQLGEILTDKEEΝAKKAVADLKAKKG 130 140 150 160 170 180 190 200 210 220 230 240 m703. pep FDAVLKQYSLΝDRTKQTGAPVGYVPLKDLEQGVPPLYQAIKDLKKGEFTATPLKΝGDFYG I I I I II II II II I I I : II II I II II II II II I II II II II II II II II I II II II II II g703 FDAVLKQYSLΝDRTKRTGAPDGYVPLKDLEQGVPPLYQAIKDLKKGEFTATPLKΝGDFYG 190 200 210 220 230 240 250 260 270 280 289 m703.pep VYYVΝDSREVKVPSFDEMKGQIAGΝLQAERIDRAVGALLGKAΝIKPAKX I II I II I II II II I II II I II I I I I I II II I II II II II II II II I II g703 VYYVΝDSREVKVPSFDEMKGQIAGΝLQAERIDRAVCALLGKAΝIKPAKX 250 260 270 280 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2353>: a703. seq 1 ATGAAAGCAA AAATCCTGAC TTCCGTTGCA CTGCTTGCCT GTTCCGGCAG 51 CCTGTTTGCC CAAACGCTGG CAACCGTCAA CGGTCAGAAA ATCGACAGTT 101 CCGTCATTGA TGCGCAGGTT GCCGCATTCC GTGCGGAAAA CAGCCGTGCC 151 GAAGACACGC CGCAACTGCG CCAATCCCTG CTGGAAAACG AAGTGGTCAA 201 CACCGTGGTC GCACAGGAAG TGAAACGCCT GAAACTCGAC CGGTCGGCAG 251 AGTTTAAAAA TGCGCTTGCC AAATTGCGTG CCGAAGCGAA AAAGTCGGGC 301 GACGACAAGA AACCGTCCTT CAAAACCGTT TGGCAGGCGG TAAAATATGG 351 CTTGAACGGC GAGGCATACG CGCTGCATAT CGCCAAAACC CAACCGGTTT 401 CCGAGCAGGA AGTAAAAGCC GCATATGACA ATATCAGCGG TTTTTACAAA 451 GGTACGCAGG AAGTCCAGTT GGGCGAAATC CTGACCGACA AGGAAGAAAA 501 TGCAAAAAAA GCGGTTGCCG ACTTGAAGGC GAAAAAAGGT TTCGATGCCG 551 TCTTGAAACA ATATTCCCTC AACGACCGTA CCAAACAGAC CGGTGCGCCG 601 GTCGGATATG TGCCGCTGAA AGATTTGGAA CAGGGTGTTC CGCCGCTTTA 651 TCAGGCAATT AAGGACTTGA AAAAAGGCGA ATTTACGGCA ACGCCGCTGA 701 AAAACGGCGA TTTCTACGGC GTTTATTATG TCAACGACAG CCGCGAGGTA 751 AAAGTGCCTT CTTTTGATGA AATGAAAGGA CAGATTGCGG GCAACCTTCA 801 GGCGGAACGG ATTGACCGTG CCGTCGGTGC ACTGTTGGGC AAGGCAAACA 851 TCAAACCTGC AAAATAA This corresponds to the amino acid sequence <SEQ ID 2354; ORF 703. a>: a703.pep 1 MKAKILTSVA LLACSGSLFA QTLATVNGQK IDSSVIDAQV AAFRAENSRA 51 EDTPQLRQSL LENEWNTW AQEVKRLKLD RSAEFKNALA KLRAEAKKSG 101 DDKKPSFKTV WQAVKYGLNG EAYALHIAKT QPVSEQEVKA AYDNISGFYK 151 GTQEVQLGEI LTDKEENAKK AVADLKAKKG FDAVLKQYSL NDRTKQTGAP 201 VGYVPLKDLE QGVPPLYQAI KDLKKGEFTA TPLKNGDFYG VYYVNDSREV 251 KVPSFDEMKG QIAGNLQAER IDRAVGALLG KANIKPAK* m703/a703 100.0% identity in 288 aa overlap 10 20 30 40 50 60 m703. pep MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFRAENSRAEDTPQLRQSL I II II I II II I II I II II II II II II II II II I II II I I II II II II II II II I I II I II a703 MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFRAENSRAEDTPQLRQSL 10 20 30 40 50 60 70 80 90 100 110 120 m703. pep LENEWNTWAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDDKKPSFKTVWQAVKYGLNG MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a 703 LENEWNTWAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDDKKPSFKTVWQAVKYGLNG 70 80 90 100 110 120 130 140 150 160 170 180 m703.pep EAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEILTDKEENAKKAVADLKAKKG MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a703 EAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEILTDKEENAKKAVADLKAKKG 130 140 150 160 170 180 190 200 210 220 230 240 m703.pep FDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQAIKDLKKGEFTATPLKNGDFYG II II II II I I II II II II II II I II I II II II I II II II II II II II I II II II I II I II a703 FDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQAIKDLKKGEFTATPLKNGDFYG 190 200 210 220 230 240 250 260 270 280 289 m703. pep VYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVGALLGKANIKPAKX II II I II II II II I II II II II II II I II II II II I II II II II II II I a703 VYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVGALLGKANIKPAKX 250 260 270 280 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2355>: a704.seq 1 ATGAAAAAAA CCTGTTTCCA CTGCGGGCTG GACGTTCCCG AAAACCTGCA 51 TCTGACCGTC CGTTACGAAA ACGAAGACCG CGAAACCTGC TGCGCCGGTT 101 GTCAGGCAGT CGCACAAAGC ATTATTGACG CGGGCTTGGG CAGTTATTAC 151 AAACAACGCA CCGCCGACGC GCAAAAAACC GAGCTGCCGC CCCAAGAAAT 201 CCTCGACCAA ATCCGCCTGT ACGACCTGCC CGAAGTCCAG TCCGACTTTG 251 TGGAAACCCA CGGCGGCACG CGCGAGGCGG TTTTAATGCT CGGCGGCATC 301 ACCTGCGCCG CCTGCGTCTG GCTGATCGAA CAGCAGCTTT TGCGTACAGA 351 CGGCATCGTC CGCATCGACC TCAATTACAG CACGCACCGC TGCCGCGTCG 401 TCTGGGACGA CGGCAAAATC CGCCTTTCCG ACATTCTGTT GAAAATCAGG 451 CAGATAGGCT ACACCGCCGC ACCCTATGAC GCGCAAAAAA TCGAAGCCGC 501 CAACCAAAAA GAACGCAAAC AATACATCGT CCGCCTCGCC GTTGCCGGGC 551 TGGGGATGAT GCAGACGATG ATGTTCGCGC TGCCGACCTA CCTTTACGGC 601 GGCGACATCG AACCCGATTT CCTGCAAATC CTCCATTGGG GCGGCTTTTT 651 AATGGTGCTG CCCGTCGTAT TCTATTGCGC CGTCCCGTTT TATCAAGGCG 701 CGCTGCGCGA CTTGAAAAAC CGCCGCGTCG GCATGGATAC GCCGATTACC 751 GTCGCCATCA TCATGACCTT TATCGCCGGC GTTTACAGCC TTGCGACAAA 801 TGCGGGGCAG GGGATGTATT TCGAATCCAT CGCGATGCTG CTGTTTTTCC 851 TGCTGGGCGG ACGCTTTATG GAACACATTG CCCGCCGTAA GGCAGGCGAT 901 GCCGCCGAGA GGCTGGTGAA GCTGATTCCT GCGTTTTGCC ATCATATGCC 951 CGATTACCCC GATACGCAGG AAACCTGCGA GGCAGCTGTC GTCAAATTGA 1001 AGGCGGGCGA TATCGTGCTG GTCAAACCGG GCGAAACCAT CCCCGTTGAC 1051 GGCACGGTGC TGGAAGGAAG CAGTGCCGTC AACGAATCTA TGCTGACCGG 1101 CGAGAGCCTG CCCGTCGCCA AAATGCCGTC TGAAAAAGTA ACCGCCGGCA 1151 CACTCAACAC GCAAAGCCCC CTGATTATAC GCACCGACCG CACCGGCGGC 1201 GGCACGCGAC TGTCGCACAT CGTCCGCCTG CTCGACCGCG CCTTAGCGCA 1251 AAAACCGCGC ACTGCCGAGT TGGCGGAACA ATACGCCTCG TCTTTCATAT 1301 TCGGCGAACT CCTGCTTGCC GTCCCCGTCT TCATCGGCTG GACGCTGTAC 1351 GCCGACGCGC ACACCGCATT GTGGATTACC GTCGCCCTGC TGGTCATTAC 1401 CTGCCCCTGC GCCTTATCGC TTGCCACGCC GACCGCGCTG GCAGCTTCTA 1451 CCGGTACGCT GGCGCGCGAA GGTATTTTAA TCGGCGGAAA GCAGGCAATC 1501 GAAACCCTCG CCCAAACCAC CGACATCATC TTCGACAAAA CCGGCACGCT 1551 GACCCAAGGC AAACCCGCCG TCCGCCGTAT CTCATTGTTG AGAGGCACAG 1601 ACGAAGCCTT TGTTCTCGCG GTGGCGCAGG CTTTAGAACA ACAGTCCGAA 1651 CATCCCCTTG CCCGCGCCAT CCTCAACTGC CGCATTTCAG ACGGCAGCGT 1701 CCCCGACATC GCTATTAAAC AACGCCTCAA CCGCATCGGC GAAGGCGTGG 1751 GCGCGCAACT GACCGTCAAC GGCGAAACAC AGGTTTGGGC ATTGGGCAGG 1801 GCATCCTATG TCGCCGAAAT TTCAGGTAAA GAACCGCAAA CAGAAGGCGG 1851 CGGCAGCGCG GTTTACCTCG GCAGTCAAAG CGGTTTCCAA GCCGTGTTCT 1901 ACCTGCAAGA CCCGCTCAAA GACAGCGCGG CGGAGGCGGT GCGGCAGTTG 1951 GCAGGCAAAA ACCTGACGCT GCACATTCTC AGCGGCGACC GTGAAACCGC 2001 CGTTGCCGAA ACCGCACGCG CCCTGGGTGT CGCGCACTAC CGCGCCCAAG 2051 CCATGCCCGA GGACAAACTG GAATACGTCA AAGCCTTGCA AAAAGAAGGG 2101 AAAAAAGTGC TGATGATAGG CGACGGCATC AACGACGCGC CCGTTTTGGC 2151 GCAGGCAGAC GTATCCGCCG CCGCAGCGGG CGGGACGGAT ATTGCGAGGG 2201 ACGGCGCGGA CATTGTGTTA TTGAACGAAG ATTTGCGTAC CGTCGCCCAC 2251 CTGCTCGATC AGGCGCGGCG CACCCGCCAT ATTATCCGGC AAAACCTGAT 2301 ATGGGCGGGC GCGTACAATA TCATTGCCGT ACCGCTTGCC GTTTTGGGCT 2351 ATGTCCAACC GTGGATAGCC GCACTGGGTA TGAGCTTCAG TTCGCTGGCG 2401 GTTTTGGGCA ACGCCCTGCG CCTTCACAAA CGGGGGAAAA TGCAGTCTGA 2451 AAAAATGCCG TCCGAACAAT GA This corresponds to the amino acid sequence <SEQ ID 2356; ORF 703>: a704.pep 1 MKKTCFHCGL DVPENLHLTV RYENEDRETC CAGCQAVAQS IIDAGLGSYY 51 KQRTADAQKT ELPPQEILDQ IRLYDLPEVQ SDFVETHGGT REAVLMLGGI 101 TCAACVWLIE QQLLRTDGIV RIDLNYSTHR CRWWDDGKI RLSDILLKIR 151 QIGYTAAPYD AQKIEAANQK ERKQYIVRLA VAGLGMMQTM MFALPTYLYG 201 GDIEPDFLQI LHWGGFLMVL PWFYCAVPF YQGALRDLKN RRVGMDTPIT 251 VAIIMTFIAG VYSLATNAGQ GMYFESIAML LFFLLGGRFM EHIARRKAGD 301 AAERLVKLIP AFCHHMPDYP DTQETCEAAV VKLKAGDIVL VKPGETIPVD 351 GTVLEGSSAV NESMLTGESL PVAKMPSEKV TAGTLNTQSP LIIRTDRTGG 401 GTRLSHIVRL LDRALAQKPR TAELAEQYAS SFIFGELLLA VPVFIGWTLY 451 ADAHTALWIT VALLVITCPC ALSLATPTAL AASTGTLARE GILIGGKQAI 501 ETLAQTTDII FDKTGTLTQG KPAVRRISLL RGTDEAFVLA VAQALEQQSE 551 HPLARAILNC RIΞDGSVPDI AIKQRLNRIG EGVGAQLTVN GETQVWALGR 601 ASYVAEISGK EPQTEGGGSA VYLGSQSGFQ AVFYLQDPLK DSAAEAVRQL 651 AGKNLTLHIL SGDRETAVAE TARALGVAHY RAQAMPEDKL EYVKALQKEG 701 KKVLMIGDGI NDAPVLAQAD VSAAAAGGTD IARDGADIVL LNEDLRTVAH 751 LLDQARRTRH IIRQNLIWAG AYNIIAVPLA VLGYVQPWIA ALGMSFSSLA 801 VLGNALRLHK RGKMQΞEKMP SEQ* m704/a704 99.8% identity in 823 aa overlap 10 20 30 40 50 60 m704. pep MKKTCFHCGLDVPEHLHLTVRYENEDRETCCAGCQAVAQΞ11DAGLGSYYKQRTADAQKT I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a704 MKKTCFHCGLDVPENLHLTVRYENEDRETCCAGCQAVAQS11DAGLGS YKQRTADAQKT 10 20 30 40 50 60 70 80 90 100 110 120 m704.pep ELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITCAACVWLIEQQLLRTDGIV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I a704 ELPPQEILD Q IRLYDLPEV Q SDFVETHGGTREAVLMLGGITCAACVWLIEQQLLRTDGIV 70 80 90 100 110 120 130 140 150 160 170 180 Iϊi704. •pep RIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYDAQKIEAANQKERKQYIVRLA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I II I I I I I I I I I I I I I I I I a704 RIDLNYSTHRCRVVWDDGKIRLΞDILLKIRQIGYTAAPYDAQKIEAANQKERKQYIVRLA 130 140 150 160 170 180 190 200 210 220 230 240 m704. • pep VAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLMVLPVVFYCAVPFYQGALRDLKN I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a704 VAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLMVLPVVFYCAVPFYQGALRDLKN 190 200 210 220 230 240 250 260 270 280 290 300 m704. .pep RRVGMDTPITVAIIMTFIAGVYSLATNAGQG YFESIAMLLFFLLGGRFMEHIARRKAGD I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a704 RRVGMDTPITVAIIMTFIAGVYSLATNAGQGMYFEΞIA LLFFLLGGRFMEHIARRKAGD 250 260 270 280 290 300 310 320 330 340 350 360 m704, ■ pep AAERLVKLIPAFCHHMPDYPDTQETCEAAVVKLKAGDIVLVKPGETIPVDGTVLEGSSAV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a704 AAERLVKLIPAFCHH PDYPDTQETCEAAVVKLKAGDIVLVKPGETIPVDGTVLEGSSAV 310 320 330 340 350 360 370 380 390 400 410 420 m704.pep NEΞMLTGESLPVAKMPSEKVTAGTLNTQΞPLIIRTDRTGGGTRLSHIVRLLDRALAQKPR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II a704 NESMLTGEΞLPVAKMPSEKVTAGTLNTQSPLIIRTDRTGGGTRLSHIVRLLDRALAQKPR 370 380 390 400 410 420 430 440 450 460 470 480 m704.pep TAELAEQYASSFIFGELLLAVPVFIGWTLYADAHTALWITVALLVITCPCALSLATPTAL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a704 TAELAEQYASSFIFGELLLAVPVFIGWTLYADAHTALWITVALLVITCPCALSLATPTAL 430 440 450 460 470 480 490 500 510 520 530 540 m704.pep AASTGTLAREGILIGGKQAIETLAQTTDIIFDKTGTLTQGKPAVRRIΞLLRGTDEAFVLA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a704 AASTGTLAREGILIGGKQAIETLAQTTDIIFDKTGTLTQGKPAVRRISLLRGTDEAFVLA 490 500 510 520 530 540 550 560 570 580 590 600 m704.pep VAQALEQQΞEHPLARAILNCRIΞDGSVPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a704 VAQALEQQSEHPLARAILNCRISDGSVPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGR 550 560 570 580 590 600 610 620 630 640 650 660 m704.pep ASYVAEISGKEPQTEGGGSAVYLGΞQSGFQAVFYLTDPLKDSAAEAVRQLAGKNLTLHIL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a704 ASYVAEISGKEPQTEGGGSAVYLGSQΞGFQAVFYLQDPLKDΞAAEAVRQLAGKNLTLHIL 610 620 630 640 650 660 670 680 690 700 710 720 m704.pep SGDRETAVAETARALGVAHYRAQAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQAD I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a704 SGDRETAVAETARALGVAHYRAQAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQAD 670 680 690 700 710 720 730 740 750 760 770 780 m704.pep VSAAAAGGTDIARDGADIVLLNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLA I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a704 VSAAAAGGTDIARDGADIVLLNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLA 730 740 750 760 770 780 790 800 810 820 m704.pep VLGYVQPWIAALGMSFΞSLAVLGNALRLHKRGKMQSEKMPSEQX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a704 VLGYVQPWIAALGMΞFSSLAVLGNALRLHKRGKMQSEKMPSEQX 790 800 810 820 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2357>: g705.seq 1 GTGTTCAATA ATTTCCttgC CTCTCTGCCG TTTATGACGG AAACACGCGC 51 TGATATGCTC ATCAGCGCGT TTTGGCCCAT GGTTAAAGCC GGCTTTACAG 101 TGTCTTtgcC TTTGGCGATC GCTTCTTTCG TTATCGGCAT GATTATTGCC 151 GTAGCCGTTG CTTTGGTAAG AATCATGCCT TCCGGCGGTA TTTTCCAAAA 201 ATGCTTGTTG AAGCTGGTGG AATTTTATAT TTCCGTCGTT CGCGGTACGC 251 CGCTGTTGGT TCAGCTTGTG ATTGTGTTTT ACGGGCTGCC GTCCGTCGGC 301 ATCTATATCA ATCCGATTCC CGCCGCCATC ATCGGCTTTT CGCTCAATGT 351 CGGCGCATAC GCTTCCGAAA CCATACGCGC GGCGATTTTG TCCGTGCCGA 401 AAGGGCAGTG GGAAGCAGGT TTCTCCATCG GTATGACCTA TATGCAGACG 451 TTCCGCCGCA TCGTCGCACC GCAGGCATTC CGCGTCGCCG TTCCGCCGTT 501 GAGCAACGAG TTTATCGGCT TGTTCAAAAA CACCTCGCTT GCCGCCGTGG 551 TAACGGTAAC GGAGCTTTTC CGTGTCGCAC AGGAAACGGC AAACCGCACT 601 TATGACTTTT TGCCTGTCTA TATCGAAGCT GCATTGGTTT ATTGGTGTTT 651 CTGTAAAGTG CTGTTTTTGA TTCAGGCGCG TTTGGAAAAA CGTTTCGACC 701 GTTATGTCGC CAAATAA This corresponds to the amino acid sequence <SEQ ID 2358; ORF 705>: g705.pep 1 VFΝΝFLASLP FMTETRADML IΞAFWPMVKA GFTVΞLPLAI AΞFVIGMIIA 51 VAVALVRIMP SGGIFQKCLL KLVEFYISVV RGTPLLVQLV IVFYGLPSVG 101 IYIΝPIPAAI IGFSLΝVGAY ASETIRAAIL SVPKGQWEAG FSIGMTYMQT 151 FRRIVAPQAF RVAVPPLΞΝE FIGLFKΝTSL AAVVTVTELF RVAQETAΝRT 201 YDFLPVYIEA ALVYWCFCKV LFLIQARLEK RFDRYVAK* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2359>: m705.seq 1 GTGTTCAATA ATTTCCTTGC TTCGCTGCCG TTTATGACGG AAACACGCGC 51 CGATATGATT GTCAGCGCGT TTTTGCCTAT GGTCAAAGCC GGCTTCGCGG 101 TCTCTCTGCC TTTGGCGGCA GCTTCTTTCG TTATCGGTAT GATGATTGCG 151 GTAGCCGTGG CTTTGGTGCG GATTATGCCC GCCGGCGGCA TCGTGCGGAA 201 AATCCTGCTG AAATTGGTGG AATTTTATAT TTCCGTCATT CGCGGTACGC 251 CGCTGTTGGT TCAGCTTGTG ATTGTGTTTT ACGGGCTGCC TTCCGTCGGC 301 ATCTATATCG ACCCGATTCC TGCCGCCATC ATCGGCTTTT CGCTCAATGT 351 CGGCGCATAC GCTTCCGAAA CCATACGCGC GGCAATTTTG TCCGTACCTA 401 AAGGCCAATG GGAAGCAGGT TTCTCCATCG GCATGACCTA TATGCAGACG 451 TTCCGCCGCA TTGTCGCGCC GCAGGCATTC CGCGTTGCCG TGCCGCCTTT 501 GAGCAACGAG TTTATCGGTT TGTTTAAAAA CACCTCGCTC GCGGCAGTCG 551 TGACGGTAAC GGAATTATTC CGCGTCGCGC AGGAAACGGC AAACCGCACT 601 TATGACTTTT TGCCCGTCTA TATCGAAGCC GCTTTGGTTT ACTGGTGTTT 651 TTGTAAAGTG CTGTTCCTGA TTCAGGCGCG TTTGGAAAAA CGTTTCGACC 701 GCTACGTCGC CAAATAA This corresponds to the amino acid sequence <SEQ ID 2360; ORF 705>: m705.pep 1 VFΝΝFLASLP FMTETRADMI VSAFLPMVKA GFAVΞLPLAA ASFVIGMMIA 51 VAVALVRIMP AGGIVRKILL KLVEFYISVI RGTPLLVQLV IVFYGLPSVG 101 IYIDPIPAAI IGFSLΝVGAY ASETIRAAIL SVPKGQWEAG FSIGMTYMQT 151 FRRIVAPQAF RVAVPPLSΝE FIGLFKΝTSL AAVVTVTELF RVAQETAΝRT 201 YDFLPVYIEA ALVYWCFCKV LFLIQARLEK RFDRYVAK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 705 shows 95.0% identity over a 238 aa overlap with a predicted ORF (ORF 705) from N gonorrhoeae: m705/g705 95.0% identity in 238 aa overlap 10 20 30 40 50 60 m705.pep VFΝΝFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFVIGMMIAVAVALVRIMP II II I I I II III I II I I I MM I I I I II I I Ml II I I I I I II I I I :| I || I || I I I I I g705 VFΝΝFLASLPFMTETRADMLIΞAFWPMVKAGFTVΞLPLAIASFVIGMIIAVAVALVRIMP 10 20 30 40 50 60 70 80 90 100 110 120 m705.pep AGGIVRKILLKLVEFYISVIRGTPLLV Q LVIVFYGLPSVGIYIDPIPAAIIGFSLΝVGAY M M I I III II I I I Ml I I I I I I II I I I I I I I II I I I I Ml || || I || I I I I I I I I g705 SGGIFQKCLLKLVEFYISVVRGTPLLVQLVIVFYGLPSVGIYINPIPAAIIGFSLNVGAY 70 80 90 100 110 120 130 140 150 160 170 180 m705.pep ASETIRAAILSVPKGQWEAGFSIG TYMQTFRRIVAPQAFRVAVPPLSNEFIGLFKNTSL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I g705 AΞETIRAAILSVPKGQWEAGFSIG TYMQTFRRIVAPQAFRVAVPPLSNEFIGLFKNTSL 130 140 150 160 170 180 190 200 210 220 230 239 m705.pep AAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFCKVLFLIQARLEKRFDRYVAKX I I I I I I I I I I I I I I II I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g705 AAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFCKVLFLIQARLEKRFDRYVAKX 190 200 210 220 230 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2361>: a705.seq 1 GTGTTCAATA ATTTCCTTGC TTCGCTGCCG TTTATGACGG AAACACGCGC 51 CGATATGATT GTCAGCGCGT TTTTGCCTAT GGTCAAAGCC GGCTTCGCGG 101 TCTCTCTGCC TTTGGCGGCA GCTTCTTTCG TTATCGGTAT GATGATTGCG 151 GTAGCCGTGG CTTTGGTGCG GATTATGCCC GCCGGCGGCA TCGTGCGGAA 201 AATCCTGCTG AAATTGGTGG AATTTTATAT TTCCGTCATT CGCGGTACGC 251 CGCTGTTGGT TCAGCTTGTG ATTGTGTTTT ACGGGCTGCC TTCCGTCGGC 301 ATCTATATCG ACCCGATTCC TGCCGCCATC ATCGGCTTTT CGCTCAATGT 351 CGGCGCATAT GCTTCCGAAA CCATACGCGC GGCAATTTTG TCCGTACCGA 401 AAGGCCAATG GGAAGCAGGT TTCTCCATCG GCATGACCTA TATGCAGACG 451 TTCCGCCGCA TCGTCGCGCC GCAGGCATTT CGCGTTGCCG TGCCGCCTTT 501 GAGCAACGAG TTTATCGGTT TGTTTAAAAA CACCTCGCTC GCGGCAGTCG 551 TGACGGTAAC GGAATTATTC CGCGTCGCGC AGGAAACGGC AAACCGCACT 601 TATGACTTTT TGCCCGTCTA TATCGAAGCC GCTTTGGTTT ACTGGTGTTT 651 TTGTAAAGTG CTGTTCCTGA TTCAGGCGCG TTTGGAAAAA CGTTTCGACC 701 GCTACGTCGC CAAATAA This corresponds to the amino acid sequence <SEQ ID 2362; ORF 705. a>: a705.pep 1 VFΝΝFLAΞLP FMTETRADMI VSAFLPMVKA GFAVSLPLAA ASFVIGMMIA 51 VAVALVRIMP AGGIVRKILL KLVEFYISVI RGTPLLVQLV IVFYGLPSVG 101 IYIDPIPAAI IGFSLΝVGAY ASETIRAAIL SVPKGQWEAG FSIGMTYMQT 151 FRRIVAPQAF RVAVPPLSΝE FIGLFKΝTΞL AAVVTVTELF RVAQETAΝRT 201 YDFLPVYIEA ALVYWCFCKV LFLIQARLEK RFDRYVAK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 705 shows 100.0% identity over a 238 aa overlap with a predicted ORF (ORF 705) from N meningitidis: a705/m705 100.0% identity in 238 aa overlap 10 20 30 40 50 60 a705.pep VFΝΝFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFVIGMMIAVAVALVRIMP I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m705 VFΝΝFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFVIGMMIAVAVALVRIMP 10 20 30 40 50 60 70 80 90 100 110 120 a705.pep AGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPΞVGIYIDPIPAAIIGFSLΝVGAY I III I I I I I I I I I II I II I I I I I I I II I II I I I I II I I I I I I I I I I I I I I I I I I I I I II I m705 AGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIYIDPIPAAIIGFSLΝVGAY 70 80 90 100 110 120 130 140 150 160 170 180 a705.pep ASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAFRVAVPPLSΝEFIGLFKΝTSL I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m705 AΞETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAFRVAVPPLSΝEFIGLFKΝTSL 130 140 150 160 170 180 190 200 210 220 230 239 a705.pep AAVVTVTELFRVAQETAΝRTYDFLPVYIEAALVYWCFCKVLFLIQARLEKRFDRYVAKX I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I m705 AAVVTVTELFRVAQETAΝRTYDFLPVYIEAALVYWCFCKVLFLIQARLEKRFDRYVAKX 190 200 210 220 230 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2363>: g706.seq 1 ATGAACTCCT CGCAACGCAA ACGCCTTTCC GgccGCTGGC TCAACTCCTA 51 CGAACGCTac cGCCaccGCC GCCTCATACA TGCCGTGCGG CTCGGCggaa 101 ccgtCCTGTT CGCCACCGCA CTCGCCCGgc tACTCCACCT CCAacacggc 151 gAATGGATAG GGAtgaCCGT CTTCGTCGTC CTCGGCATGC TCCAGTTCCA 201 AGGCgcgatt tActccaacg cggtgGAacg taTGctcggt acggtcatcg 251 ggctgGGCGC GGGTTTGGgc gTTTTATGGC TGAACCAGCA TTAtttccac 301 ggcaacCTcc tcttctacct gaccatcggc acggcaagcg cactggccgg 351 ctGGGCGGCG GTCGGCAAAA acggctacgt ccctatgctg GCGGGGctgA 401 CGATGTGCAT gctcatcggc gACAACGGCA GCGAATGGCT CGACAGCGGC 451 CTGATGCGCG CGATGAACGT CCTCATCGGC GCCGCCATCG CCATTGCCGC 501 CGCCAAACTG CTGCCGCTGA AATCCACACT GATGTGGCGT TTCATGCTTG 551 CCGACAACCT GGCCGACTGC AGCAAAATGA TTGCCGAAAT CAGCAACGGC 601 AGGCGTATGA CGCGCGAACG TTTGGAGCAG AATATGGTCA AAATGCGCCA 651 AATCAACGCA CGCATGGTCA AAAGCCGCAG CCACCTCGCC GCCACATCGG 701 GCGAAAGCCG CATCAGCCCC TCCATGATGG AAGCCATGCA GCACGCCCAC 751 CGCAAAATCG TCAACACCAC CGAGCTGCTC CTGACCACCG CCGCCAAGCT 801 GCAATCTCCC AAACTCAACG GCAGCGAAAT CCGGCTGCTC GACCGCCACT 851 TCACACTGCT CCAAACCGAC CTGCAACAAA CCGCCGCCCT CATCAACGGC 901 AGACACGCCC GCCGCATCCG CATCGACACC GCCATCAACC CCGAACTGGA 951 AGCCCTCGCC GAACACCTCC ACTACCAATG GCAGGGCTTC CTCTGGCTCA 1001 GCACCAATAT GCGTCAGGAA ATTTCCGCCC TCGTCATCCT GCTGCAACGC 1051 ACCCGCCGCA AATGGCTGGA TGCCCACGAA CGCCAACACC TGCGCCAAAG 1101 CCTGCTTGAA ACACGGGAAC ACGGCTGA This corresponds to the amino acid sequence <SEQ ID 2364; ORF 706.ng>: g706.pep 1 MΝSSQRKRLΞ GRWLΝSYERY RHRRLIHAVR LGGTVLFATA LARLLHLQHG 51 EWIGMTVFVV LG LQFQGAI YΞΝAVERMLG TVIGLGAGLG VLWLΝQHYFH 101 GΝLLFYLTIG TASALAGWAA VGKΝGYVPML AGLTMCMLIG DΝGSEWLDSG 151 LMRAMΝVLIG AAIAIAAAKL LPLKSTLMWR FMLADΝLADC SKMIAEISΝG 201 RRMTRERLEQ ΝMVKMRQIΝA RMVKSRSHLA ATSGESRISP SMMEAMQHAH 251 RKIVΝTTELL LTTAAKLQSP KLΝGΞEIRLL DRHFTLLQTD LQQTAALIΝG 301 RHARRIRIDT AIΝPELEALA EHLHYQWQGF LWLΞTΝMRQE ISALVILLQR 351 TRRKWLDAHE RQHLRQΞLLE TREHG* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2365>: m706.seq 1 ATGAACACCT CGCAACGCAA CCGCCTCGTC AGCCGCTGGC TCAACTCCTA 51 CGAACGCTAC CGCTACCGCC GCCTCATCCA CGCCGTCCGG CTCGGCGGGG 101 CCGTCCTGTT CGCCACCGCC TCCGCCCGGC TGCTCCACCT CCAACACGGC 151 GAGTGGATAG GGATGACCGT CTTCGTCGTC CTCGGCATGC TCCAGTTTCA 201 AGGGGCGATT TACTCCAAGG CGGTGGAACG TATGCTCGGC ACGGTCATCG 251 GGCTGGGCGC GGGTTTGGGC GTTTTATGGC TGAACCAGCA TTATTTCCAC 301 GGCAACCTCC TCTTCTACCT CACCGTCGGC ACGGCAAGCG CACTGGCCGG 351 CTGGGCGGCG GTCGGCAAAA ACGGCTACGT CCCTATGCTG GCAGGGCTGA 401 CGATGTGTAT GCTCATCGGC GACAACGGCA GCGAATGGCT CGACAGCGGA 451 CTCATGCGCG CCATGAACGT CCTCATCGGC GCGGCCATCG CCATCGCCGC 501 CGCCAAACTG CTGCCGCTGA AATCCACACT GATGTGGCGT TTCATGCTTG 551 CCGACAACCT GGCCGACTGC AGCAAAATGA TTGCCGAAAT CAGCAACGGC 601 AGGCGCATGA CCCGCGAACG CCTCGAGGAG AACATGGCGA AAATGCGCCA 651 AATCAACGCA CGCATGGTCA AAAGCCGCAG CCATCTCGCC GCCACATCGG 701 GCGAAAGCCG CATCAGCCCC GCCATGATGG AAGCCATGCA GCACGCCCAC 751 CGTAAAATCG TCAACACCAC CGAGCTGCTC CTGACCACCG CCGCCAAGCT 801 GCAATCTCCC AAACTCAACG GCAGCGAAAT CCGGCTGCTT GACCGCCACT 851 TCACACTGCT CCAAACCGAC CTGCAACAAA CCGTCGCCCT TATCAACGGC 901 AGACACGCCC GCCGCATCCG CATCGACACC GCCATCAACC CCGAACTGGA 951 AGCCCTCGCC GAACACCTCC ACTACCAATG GCAGGGCTTC CTCTGGCTCA 1001 GCACCAATAT GCGTCAGGAA ATTTCCGCCC TCGTCATCCT GCTGCAACGC 1051 ACCCGCCGCA AATGGCTGGA TGCCCACGAA CGCCAACACC TGCGCCAAAG 1101 CCTGCTTGAA ACACGGGAAC ACGGCTGA This corresponds to the amino acid sequence <SEQ ID 2366; ORF 706>: 1 MΝTSQRΝRLV SRWLΝSYERY RYRRLIHAVR LGGAVLFATA SARLLHLQHG 51 EWIGMTVFVV LGMLQFQGAI YΞKAVERMLG TVIGLGAGLG VLWLΝQHYFH 101 GΝLLFYLTVG TASALAGWAA VGKΝGYVPML AGLTMCMLIG DΝGΞEWLDSG 151 LMRAMΝVLIG AAIAIAAAKL LPLKSTLMWR FMLADΝLADC SKMIAEIΞΝG 201 RRMTRERLEE ΝMAKMRQIΝA RMVKSRSHLA ATSGESRISP AMMEAMQHAH 251 RKIVΝTTELL LTTAAKLQSP KLΝGΞEIRLL DRHFTLLQTD LQQTVALIΝG 301 RHARRIRIDT AINPELEALA EHLHYQWQGF LWLSTNMRQE ISALVILLQR 351 TRRKWLDAHE RQHLRQSLLE TREHG* m706/g706 96.5% identity in 375 aa overlap 10 20 30 40 50 60 m706. pep MNTSQRNRLVΞRWLNSYERYRYRRLIHAVRLGGAVLFATASARLLHLQHGEWIGMTVFVV I I : I I Ml I : I I I I I I I I I I M I I I I I I I I I M I I I I I I I I I I I I I I I I I I I I I I I I I g706 MNSSQRKRLSGRWLNSYERYRHRRLIHAVRLGGTVLFATALARLLHLQHGEWIGMTVFVV 10 20 30 40 50 60 70 80 90 100 110 120 m706.pep LGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGNLLFYLTVGTASALAGWAA I I I I I I I I I I I Ml I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I g 706 LGMLQFQGAIYSNAVERMLGTVIGLGAGLGVLWLNQHYFHGNLLFYLTIGTASALAGWAA 70 80 90 100 110 120 130 140 150 160 170 180 m706.pep VGKNGYVPMLAGLTMCMLIGDNGSEWLDΞGLMRAMNVLIGAAIAIAAAKLLPLKSTLMWR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g 706 VGKNGYVPMLAGLTMCMLIGDNGΞEWLDΞGLMRAMNVLIGAAIAIAAAKLLPLKSTLMWR 130 140 150 160 170 180 190 200 210 220 230 240 m706.pep FMLADNLADCSKMIAEISNGRRMTRERLEENMAKMRQINARMVKSRSHLAATΞGESRISP I I I I I I I I I I I I I I I I I II I I I I I I I I I I : I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I g706 FMLADNLADCSKMIAEISNGRRMTRERLEQNMVKMRQINARMVKSRSHLAATΞGESRISP 190 200 210 220 230 240 250 260 270 280 290 300 m706.pep AMMEAMQHAHRKIVNTTELLLTTAAKLQΞPKLNGΞEIRLLDRHFTLLQTDLQQTVALING :| I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I g706 SMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSEIRLLDRHFTLLQTDLQQTAALING 250 260 270 280 290 300 310 320 330 340 350 360 m706.pep RHARRIRIDTAINPELEALAEHLHYQWQGFLWLSTNMRQEISALVILLQRTRRKWLDAHE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g706 RHARRIRIDTAINPELEALAEHLHYQWQGFLWLSTNMRQEIΞALVILLQRTRRKWLDAHE 310 320 330 340 350 360 370 m706.pep RQHLRQSLLETREHGX I I I II I I I I I I I II I I g706 RQHLRQSLLETREHGX 370 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2367>: a706.seq 1 ATGAACACCT CGCAACGCAA CCGCCTCGTC AGCCGCTGGC TCAACTCCTA 51 CGAACGCTAC CGCTACCGCC GCCTCATCCA CGCCGTCCGG CTCGGCGGGG 101 CCGTCCTGTT CGCCACCGCC TCCGCCCGGC TGCTCCACCT CCAACACGGC 151 GAGTGGATAG GGATGACCGT CTTCGTCGTC CTCGGCATGC TCCAGTTTCA 201 AGGGGCGATT TACTCCAAGG CGGTGGAACG TATGCTCGGC ACGGTCATCG 251 GGCTGGGCGC GGGTTTGGGC GTTTTATGGC TGAACCAGCA TTATTTCCAC 301 GGCAACCTCC TCTTCTACCT CACCGTCGGC ACGGCAAGCG CACTGGCCGG 351 CTGGGCGGCG GTCGGCAAAA ACGGCTACGT CCCTATGCTG GCGGGGCTGA 401 CGATGTGCAT GCTCATCGGC GACAACGGCA GCGAATGGTT CGACAGCGGC 451 CTGATGCGCG CGATGAACGT CCTCATCGGC GCGGCCATCG CCATCGCCGC 501 CGCCAAACTG CTGCCGCTGA AATCCACACT GATGTGGCGT TTCATGCTTG 551 CCGACAACCT GACCGACTGC AGCAAAATGA TTGCCGAAAT CAGCAACGGC 601 AGGCGCATGA CCCGCGAACG CCTCGAAGAG AACATGGCGA AAATGCGCCA 651 AATCAACGCA CGCATGGTCA AAAGCCGCAG CCACCTCGCC GCCACATCGG 701 GCGAAAGCCG CATCAGCCCC GCCATGATGG AAGCCATGCA GCACGCCCAC 751 CGTAAAATTG TCAACACCAC CGAGCTGCTC CTGACCACCG CCGCCAAGCT 801 GCAATCTCCC AAACTCAACG GCAGCGAAAT CCGGCTGCTT GACCGCCACT 851 TCACACTGCT CCAAACCGAC CTGCAACAAA CCGTCGCCCT TATCAACGGC 901 AGACACGCCC GCCGCATCCG CATCGACACC GCCATCAACC CCGAACTGGA 951 AGCCCTCGCC GAACACCTCC ACTACCAATG GCAGGGCTTC CTCTGGCTCA 1001 GCACCAATAT GCGTCAGGAA ATTTCCGCCC TCGTCATCCT GCTGCAACGC 1051 ACCCGCCGCA AATGGCTGGA TGCCCACGAA CGCCAACACC TGCGCCAAAG 1101 CCTGCTTGAA ACACGGGAAC ACAGTTGA This corresponds to the amino acid sequence <SEQ ID 2368; ORF 706.a>: a706.pep 1 MNTSQRNRLV SRWLNSYERY RYRRLIHAVR LGGAVLFATA SARLLHLQHG 51 EWIGMTVFVV LGMLQFQGAI YSKAVERMLG TVIGLGAGLG VLWLNQHYFH 101 GNLLFYLTVG TASALAGWAA VGKNGYVPML AGLTMCMLIG DNGSEWFDSG 151 LMRAMNVLIG AAIAIAAAKL LPLKSTLMWR FMLADNLTDC SKMIAEISNG 201 RRMTRERLEE NMAKMRQINA RMVKSRSHLA ATSGESRISP AMMEAMQHAH 251 RKIVNTTELL LTTAAKLQSP KLNGSEIRLL DRHFTLLQTD LQQTVALING 301 RHARRIRIDT AINPELEALA EHLHYQWQGF LWLSTNMRQE ISALVILLQR 351 TRRKWLDAHE RQHLRQSLLE TREHS* a706/m706 99.5% identity in 374 aa overlap 10 20 30 40 50 60 a706. pep MNTSQRNRLVSRWLNSYERYRYRRLIHAVRLGGAVLFATASARLLHLQHGEWIGMTVFW I II I I I II II I II I II II II I II II II II I II II I II II II II II II I II II II II II II m706 MNTSQRNRLVSRWLNSYERYRYRRLIHAVRLGGAVLFATASARLLHLQHGEWIGMTVFW 10 20 30 40 50 60 70 80 90 100 110 120 a706. pep LGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGNLLFYLTVGTASALAGWAA I I I II II II II I II II II I I II I II II II I II II II I II II I I II II I II I II II I II II m706 LGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGNLLFYLTVGTASALAGWAA 70 80 90 100 110 120 130 140 150 160 170 180 a706. pep VGKNGYVPMLAGLTMCMLIGDNGSEWFDSGLMRAMNVLIGAAIAIAAAKLLPLKSTLMWR I I II I M M I II II II II I I II llll: II I II II II I II I II II I II I II I II II II I II m706 VGKNGYVPMLAGLTMCMLIGDNGSEWLDSGLMRAMNVLIGAAIAIAAAKLLPLKSTLMWR 130 140 150 160 170 180 190 200 210 220 230 240 a706.pep FMLADNLTDCSKMIAEISNGRRMTRERLEENMAKMRQINARMVKSRSHLAATSGESRISP I I II II M II I II I II I I II I II II I II II II II I II I II II II II I II II II II II II I m706 FMLADNLADCSKMIAEISNGRRMTRERLEENMAKMRQINARMVKSRSHLAATSGESRISP 190 200 210 220 230 240 250 260 270 280 290 300 a706.pep AMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSEIRLLDRHFTLLQTDLQQTVALING II I II II II II I II II II I II II II II II I II II I II II II II II I II I II I II II I II I m706 AMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSEIRLLDRHFTLLQTDLQQTVALING 250 260 270 280 290 300 310 320 330 340 350 360 a706.pep RHARRIRIDTAINPELEALAEHLHYQWQGFLWLSTNMRQEISALVILLQRTRRKWLDAHE I I I I II II I II II II II I I I II II II I II I II I I II I II II II II I II I II II I II II II m706 RHARRIRIDTAINPELEALAEHLHYQWQGFLWLSTNMRQEISALVILLQRTRRKWLDAHE 310 320 330 340 350 360 370 a706.pep RQHLRQSLLETREHSX II II II II II I II M m706 RQHLRQSLLETREHGX 370 g707. seq not found g707.peρ not found The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2369>: 707. seq 1 ATGGAAATTA TTAACGATGC AGAACTTATC CGTTCCATGC AGCGTCAGCA 51 GCACATAGAT GCTGAATTGT TAACTGATGC AAATGTCCGT TTCGAGCAAC 101 CATTGGAGAA GAACAATTAT GTCCTGAGTG AAGATGAAAC ACCGTGTACT 151 CGGGTAAATT ACATTAGTTT AGATGATAAG ACGGTGCGCA AATTTTCTTT 201 TCTTCCTTCT GTGCTCATGA AAGAAACAGC TTTTAAAACT GGGATGTGTT 251 TAGGTTCCAA TAATTTGAGC AGGCTACAAA AAGCCGCGCA ACAGATACTG 301 ATCGTGCGTG GCTACCTCAC TTCCCAAGCT ATTATCCAAC CACAGAATAT 351 GGATTCGGGA ATTCTGAAAT TACGGGTATC AGCAGGCGAA ATAGGGGATA 401 TCCGCTATGA AGAAAAACGG GATGGGAAGT CTGCCGAGGG CAGTATTAGT 451 GCATTCAATA ACAAATTTCC CTTATATAGG AACAAAATTC TCAATCTTCG 501 CGATGTAGAG CAGGGCTTGG AAAACCTGCG TCGTTTGCCG AGTGTTAAAA 551 CAGATATTCA GATTATACCG TCCGAAGAAG AAGGCAAAAG CGATTTACAG 601 ATCAAATGGC AGCAGAATAA ACCCATACGG TTCAGTATCG GTATAGATGA 651 TGCGGGCGGC AAAACGACCG GCAAATATCA AGGAAATGTC GCTTTATCGT 701 TCGATAACCC TTTGGGCTTA AGCGATTTGT TTTATGTTTC ATATGGACGC 751 GGTTTGGCGC ACAAAACGGA CTTGACTGAT GCCACCGGTA CGGAAACTGA 801 AAGCGGATCC AGAAGTTACA GCGTGCATTA TTCGGTGCCC GTAAAAAAAT 851 GGCTGTTTTC TTTTAATCAC AATGGACATC GTTACCACGA AGCAACCGAA 901 GGCTATTCCG TCAATTACGA TTACAACGGC AAACAATATC AGAGCAGCCT 951 GGCCGCCGAG CGCATGCTTT GGCGTAACAG ACTTCATAAA ACTTCAGTCG 1001 GAATGAAATT ATGGACACGC CAAACCTATA AATACATCGA CGATGCCGAA 1051 ATCGAAGTAC AACGCCGCCG CTCTGCAGGC TGGGAAGCCG AATTGCGCCA 1101 CCGTGCTTAC CTCAACCGTT GGCAGCTTGA CGGCAAGTTG TCTTACAAAC 1151 GCGGGACCGG CATGCGCCAA AGTATGCCTG CACCGGAAGA AAACGGCGGC 1201 GATATTCTTC CAGGTACATC TCGTATGAAA ATCATTACTG CCAGTTTGGA 1251 CGCAGCCGCC CCATTTATTT TAGGCAAACA GCAGTTTTTC TACGCAACCG 1301 CCATTCAAGC TCAATGGAAC AAAACGCCGT TGGTTGCCCA AGATAAATTG 1351 TCAATCGGCA GCCGCTACAC CGTTCGCGGA TTTGATGGGG AGCAGAGTCT 1401 TTTCGGAGAG CGAGGTTTCT ACTGGCAGAA TACTTTAACT TGGTATTTTC 1451 ATCCGAACCA TCAGTTCTAT CTCGGTGCGG ACTATGGCCG CGTATCTGGC 1501 GAAAGTGCAC AATATGTATC GGGCAAGCAG CTGATGGGTG CAGTGGTCGG 1551 CTTCAGAGGA GGGCATAAAG TAGGCGGTAT GTTTGCTTAT GATCTGTTTG 1601 CCGGCAAGCC GCTTCATAAA CCCAAAGGCT TTCAGACGAC CAACACCGTT 1651 TACGGCTTCA ACTTGAATTA CAGTTTCTAA This corresponds to the amino acid sequence <SEQ ID 2370; ORF 707>: m707.pep 1 MEIINDAELI RSMQRQQHID AELLTDANVR FEQPLEKNNY VLΞEDETPCT 51 RVNYISLDDK TVRKFSFLPS VLMKETAFKT GMCLGSNNLS RLQKAAQQIL 101 IVRGYLTSQA IIQPQNMDSG ILKLRVSAGE IGDIRYEEKR DGKSAEGSIS 151 AFNNKFPLYR NKILNLRDVE QGLENLRRLP SVKTDIQIIP SEEEGKSDLQ 201 IKWQQNKPIR FΞIGIDDAGG KTTGKYQGNV ALSFDNPLGL SDLFYVSYGR 251 GLAHKTDLTD ATGTETESGS RSYΞVHYSVP VKKWLFSFNH NGHRYHEATE 301 GYSVNYDYNG KQYQSSLAAE RMLWRNRLHK TSVGMKLWTR QTYKYIDDAE 351 IEVQRRRSAG WEAELRHRAY LNRWQLDGKL SYKRGTGMRQ SMPAPEENGG 401 DILPGTSRMK IITASLDAAA PFILGKQQFF YATAIQAQWN KTPLVAQDKL 451 ΞIGSRYTVRG FDGEQΞLFGE RGFYWQNTLT WYFHPNHQFY LGADYGRVSG 501 EΞAQYVSGKQ LMGAVVGFRG GHKVGGMFAY DLFAGKPLHK PKGFQTTNTV 551 YGFNLNYSF* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2371>: a707.seq 1 ΝTGAAAGAAA CAGCTTTTAA AACTGGGATG TGTTTAGGTT CCAATAATTT 51 GAGCAGGCTA CAAAAAGCCG CGCAACAGAT ACTGATTGTG CGTGGCTACC 101 TCACTTCCCA AGCTATTATC CAACCACAGA ATATGGATTC GGGAATTCTG 151 AAATTACGGG TATCAGCAGG CGAAATAGGN GATATCCGCT ATGAAGAAAA 201 ACGGGATGNG AAGTCTGCCG AGGGCAGTAT TAGTGCATTC AATAACAAAN 251 TTCCCTTATA TAGGAACAAA ATTCTCAATC TTCGCGATGT AGAGCAGGGC 301 TTGGAAAACC TGCGTCGTTT GCCGAGTGTT AAAACAGATA TTCAGATTAT 351 ACCGTCCGAA GAAGAAGGCA AAAGCGATTT ACAGATCAAA TGGCAGCAGA 401 ATAAACCCAT ACGGTTCAGT ATCGGTATAG ATGATGCGGG CGGCAAAACG 451 ACCGGCAAAT ATCAAGGAAA TGTCGCTTTA TCGTNCGATA ACCCTTTGGG 501 NTTAAGCGAT TNGTTTTATG TTTCATATGG ACGCGGTTTG GTGCACAAAA 551 CGGACTTGAC TGNTGCCACC GGTACGGAAA CTGAAAGCGG ATCCAGAAGT 601 TACAGCGTGC ATTATTCGGT GNNCGTAAAA AAATGGCTGT TTTCTTTTAA 651 TCACAATGGA CATCGTTACC ACGAAGCAAC CGAAGGCTAT TCCGTCAATT 701 ACGATTACAA CGGCAAACAA TATCAGAGCA GCCTGGCCGC CGAGCGCATG 751 CTTTGGNNNN NNAGNTTTCN TNAAACTTCA GTCNGAATGA AATTATGGAC 801 ACGCCAAACC TATAAATACA TCGACGATGC CGAAATCGAA GTGCAACGCC 851 GCCGCTCTGC AGGCTGGGAA GCCGAATTGC GCCACCGTGC TTACCTCNAC 901 CGTTGGCAGC TTGACGGCAA GTTGTCTTAC AAACGCGGGA CCGGCATGCG 951 CCAAAGTATG CCCGCACCTG AAGAAAACGG CGGCGGTACT ATTCCAGNCA 1001 NATCCCGTAT GAAAATCATA ACCGCCGGAT TGGATGCAGC GGCCCCGTNT 1051 ATGTTGGGCA AACAGCAGTT TTTCTACGCA ACCGCCATTC AAGCTCAATG 1101 GAACAAAACG CCTTTGGTTG CCCAAGACAA GTTGTCTATC GGCAGCCGCT 1151 ACACCGTTCG CGGATTTGAT GGGGAGCAGA GTCTTTTCGG AGAGCGAGGT 1201 TTCTACTGGC AGAATACTTT AACTTGGTAT TTTCATCCGA ACCATCAGTT 1251 CTATCTCGGT GCGGACTATG GCCGCGTATC TGGCGAAAGT GCACAATATG 1301 TATCGGGCAA GCAGCTGATG GGTGCAGTGG TCGGCTTCAG AGGAGGGCAT 1351 AAAGTAGGCG GTATGTTTGC TTATGATCTG TTTGCCGGCA AGCCGCTTCA 1401 TAAACCCAAA GGCTTTCAGA CGACCAACAC CGTTTACGGC TTCAACTTGA 1451 ATTACAGTTT CTAA This corresponds to the amino acid sequence <SEQ ID 2372; ORF 707. a>: a707.pep 1 XKETAFKTGM CLGSNNLSRL QKAAQQILIV RGYLTSQAII QPQNMDSGIL 51 KLRVSAGEIG DIRYEEKRDX KSAEGSISAF NNKXPLYRNK ILNLRDVEQG 101 LENLRRLPSV KTDIQIIPSE EEGKSDLQIK WQQNKPIRFS IGIDDAGGKT 151 TGKYQGNVAL SXDNPLGLSD XFYVSYGRGL VHKTDLTXAT GTETESGSRS 201 YSVHYSVXVK KWLFSFNHNG HRYHEATEGY SVNYDYNGKQ YQSSLAAERM 251 LWXXXFXXTS VXMKLWTRQT YKYIDDAEIE VQRRRSAGWE AELRHRAYLX 301 RWQLDGKLSY KRGTGMRQSM PAPEENGGGT IPXXSRMKII TAGLDAAAPX 351 MLGKQQFFYA TAIQAQWNKT PLVAQDKLSI GSRYTVRGFD GEQSLFGERG 401 FYWQNTLTWY FHPNHQFYLG ADYGRVSGES AQYVSGKQLM GAWGFRGGH 451 KVGGMFAYDL FAGKPLHKPK GFQTTNTVYG FNLNYSF* a707/m707 95.3% identity in 486 aa overlap 10 20 30 a707. pep XKETAFKTGMCLGSNNLSRLQKAAQQILIVR II I II II II II II II II I II I II II I II II m707 EDETPCTRVNYISLDDKTVRKFSFLPSVLMKETAFKTGMCLGSNNLSRLQKAAQQILIVR 50 60 70 80 90 100 40 50 60 70 80 90 a707.pep GYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEKRDXKSAEGSIΞAFNNKXPLYRNKI I II II I II I II II II I II I I II II II I II II II II II I II I I II II I I I I I II II I II m707 GYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEKRDGKSAEGSISAFNNKFPLYRNKI 110 120 130 140 150 160 100 110 120 130 140 150 a707.pep LNLRDVEQGLENLRRLPSVKTDIQIIPSEEEGKSDLQIKWQQNKPIRFSIGIDDAGGKTT I II II II II II II II II I II I II II II I II II II II II II II II I II I II I II II II I II m707 LNLRDVEQGLENLRRLPSVKTDIQIIPSEEEGKSDLQIKWQQNKPIRFSIGIDDAGGKTT 170 180 190 200 210 220 160 170 180 190 200 210 a707.pep GKYQGNVALSXDNPLGLSDXFYVSYGRGLVHKTDLTXATGTETESGSRSYSVHYSVXVKK II II II I II I II II II II I II I I II I I: I II II I II II II II II I I II II I II III m707 GKYQGNVALSFDNPLGLSDLFYVSYGRGLAHKTDLTDATGTETESGSRSYSVHYSVPVKK 230 240 250 260 270 280 220 230 240 250 260 270 a707.pep WLFΞFNHNGHRYHEATEGYSVNYDYNGKQYQSSLAAERMLWXXXFXXTSVXMKLWTRQTY I II I I I I II II I II II I I I II I I II I I I II II II II II II I : III m707 WLFSFNHNGHRYHEATEGYSVNYDYNGKQYQSSLAAERMLWRNRLHKTSVGMKLWTRQTY 290 300 310 320 330 340 280 290 300 310 320 330 a707. pep KYIDDAEIEVQRRRSAGWEAELRHRAYLXRWQLDGKLSYKRGTGMRQSMPAPEENGGGTI II II II II II II II II II I I II I II I II I II II II II II II I II II I I II II II II : m707 KYIDDAEIEV Q RRRSAGWEAELRHRAYLNRWQLDGKLSYKRGTGMRQSMPAPEENGGDIL 350 360 370 380 390 400 340 350 360 370 380 390 a707.pep PXXSRMKIITAGLDAAAPXMLGKQQFFYATAIQAQWNKTPLVAQDKLSIGSRYTVRGFDG I : I I II I II I : I II I II : II I II II I II II II II II II II I II I II I II II II II I I I m707 PGTSRMKIITASLDAAAPFILGKQQFFYATAIQAQWNKTPLVAQDKLSIGSRYTVRGFDG 410 420 430 440 450 460 400 410 420 430 440 450 a707.pep EQSLFGERGFYWQNTLTWYFHPNHQFYLGADYGRVSGESAQYVSGKQLMGAWGFRGGHK II II II II I II I II II II II I II II I I II I II II II II II II II II II II II II I I II II m707 EQSLFGERGFYWQNTLTWYFHPNHQFYLGADYGRVSGESAQYVSGKQLMGAWGFRGGHK 470 480 490 500 510 520 460 470 480 a707. pep VGGMFAYDLFAGKPLHKPKGFQTTNTVYGFNLNYSFX I II I II II I I II II II I II II I II II I II I II II I II m707 VGGMFAYDLFAGKPLHKPKGFQTTNTVYGFNLNYSFX 530 540 550 560 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2373>: g708.seq 1 ATGCCTTTTA AGCCATCCAA ACGAATCTCT TTATTACTTG TTCTTGCCTT 51 GGGCGCGTGC AGCACTTCCT ACCGCCCCTC GCGGGCAGAA AAAGCCAATC 101 AGGTTTCCAA TATCAAAACC CAGTTGGCGA TGGAATATAT GCGCGGTCAG 151 GACTACCGTC AGGCAACGGC AAGTATTGAA GATGCCTTGA AATCGAACCC 201 TAAAAACGAA CTTGCCTGGC TGGTCCGTGC CGAAATCTAT CAATACCTGA 251 AAGTTAACGA CAAGGCGCAG GAAAGTTTCC GGCAAGCCCT CTCCATCAAA 301 CCCGACAGTG CCGAAATCAA CAACAACTAC GGCTGGTTCC TGTGCGGCAG 351 GCTCAACCGC CCTGCCGAAT CTATGGCATA TTTCGACAAA GCCCTGGCCG 401 ACCCCACCTA CCCGACCCCT TATATTGCCA ACCTGAATAA AGGTATATGC 451 AGCGCAAAAC AGGGGCAATT CGGATTGGCG GAAGCCTATT TGAAACGTTC 501 CCTCGCCGCC CAGCCGCAGT TCCCACCCGC ATTTAAAGAA CTGGCGCGCA 551 CCAAAATGCT GGCCGGGCAG TTGGGCGATG CCGATTACTA CTTTAAAAAA 601 TACCAAAGCA GGGTAGAAGT CCTTCAGGCC GATGATTTGC TGCTAGGCTG 651 GAAAATTGCC AAAGCCCTCG GCAACGTGCA GGCGGCATAC GAATATGAAG 701 CACAATTGCA GGCAAATTTC CCCTACTCGG AAGAATTGCA AACCGTCCTC 751 ACCGGTCAAT AA This corresponds to the amino acid sequence <SEQ ID 2374; ORF 708. ng>: g708.pep 1 MPFKPSKRIΞ LLLVLALGAC STSYRPSRAE KAΝQVSΝIKT QLAMEYMRGQ 51 DYRQATASIE DALKSΝPKΝE LAWLVRAEIY QYLKVΝDKAQ ESFRQALSIK 101 PDSAEIΝΝΝY GWFLCGRLΝR PAESMAYFDK ALADPTYPTP YIAΝLΝKGIC 151 SAKQGQFGLA EAYLKRSLAA QPQFPPAFKE LARTKMLAGQ LGDADYYFKK 201 YQSRVEVLQA DDLLLGWKIA KALGΝVQAAY EYEAQLQAΝF PYSEELQTVL 251 TGQ* The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 2375>: m708. seq 1 ATGCCTTTTA AGCCATCCAA ACGAATCTCT TTATTACTCG TTCTTGCCTT 51 GGGCGCGTGC AGCACTTCCT ACCGCCCCTC GCGGGCAGAA AAAGCCAATC 101 AGGTTTCCAA TATCAAAACC CAGTTGGCAA TGGAATATAT GCGCGGTCAG 151 GACTACCGTC AGGCGACGGC AAGTATTGAA GACGCCCTGA AATCGGACCC 201 TAAAAACGAG CTTGCCTGGC TGGTCCGTGC CGAAATCTAT CAATACCTGA 251 AAGTTAACGA CAAGGCGCAG GAAAGTTTCC GGCAAGCCCT CTCCATCAAA 301 CCCGACAGTG CCGAAATCAA CAACAACTAC GGTTGGTTCC TATGCGGCAG 351 GCTCAACCGC CCTGCCGAAT CTATGGCATA TTTCGACAAA GCTCTGGCCG 401 ACCCCACCTA CCCGACCCCT TATATTGCCA ACCTGAATAA AGGCATATGC 451 AGCGCAAAAC AGGGGCAATT CGGATTGGCG GAAGCCTATT TGAAACGTTC 501 CCTCGCCGCC CAGCCGCAGT TCCCACCCGC ATTTAAAGAA CTGGCGCGCA 551 CCAAAATGCT GGCCGGGCAG TTGGGCGATG CCGATTACTA CTTTAAAAAA 601 TACCAAAGCA GGGTAGAAGT CCTTCAGGCC GATGATTTGC TGCTAGGCTG 651 GAAAATTGCC AAAGCCCTCG GCAACGCACA GGCGGCATAC GAATATGAAG 701 CACAATTGCA GGCGAATTTC CCCTACTCGG AAGAATTGCA AACCGTCCTC 751 ACCGGTCAAT AA This corresponds to the amino acid sequence <SEQ ID 2376; ORF 708>: m708.pep 1 MPFKPSKRIS LLLVLALGAC STSYRPSRAE KANQVΞNIKT QLAMEYMRGQ 51 DYRQATASIE DALKSDPKNE LAWLVRAEIY QYLKVNDKAQ ESFRQALSIK 101 PDSAEINNNY GWFLCGRLNR PAESMAYFDK ALADPTYPTP YIANLNKGIC 151 SAKQGQFGLA EAYLKRSLAA QPQFPPAFKE LARTKMLAGQ LGDADYYFKK 201 YQSRVEVLQA DDLLLGWKIA KALGNAQAAY EYEAQLQANF PYSEELQTVL 251 TGQ* m708/g708 99.2% identity in 253 aa overlap 10 20 30 40 50 60 m708.pep MPFKPSKRIΞLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAMEYMRGQDYRQATASIE II I I I I I I I I I I I II I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g708 MPFKPSKRISLLLVLALGACSTSYRPΞRAEKANQVSNIKTQLAMEYMRGQDYRQATASIE 10 20 30 40 50 60 70 80 90 100 110 120 m708.pep DALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPDSAEINNNYGWFLCGRLNR I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g708 DALKSNPKNELAWLVRAEIYQYLKVNDKAQEΞFRQALSIKPDSAEINNNYGWFLCGRLNR 70 80 90 100 110 120 130 140 150 160 170 180 m708.pep PAEΞMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLAEAYLKRΞLAAQPQFPPAFKE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g708 PAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLAEAYLKRSLAAQPQFPPAFKE 130 140 150 160 170 180 190 200 210 220 230 240 m708. pep LARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWKI KALGNAQAAYEYEAQLQANF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I g708 LARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWKIAKALGNVQAAYEYEAQLQANF 190 200 210 220 230 240 250 m708.pep PYSEELQTVLTGQX g708 PYSEELQTVLTGQX 250 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2377>: a708.seq 1 ATGCCTTTTA AGCCATCCAA ACGAATCTCT TTATTACTTG TCCTTGCCTT 51 GGGCGCGTGC AGCACTTCCT ACCGCCCCTC GCGGGCAGAA AAAGCCAATC 101 AGGTTTCCAA TATCAAAACC CAGTTGGCAA TGGAATATAT GCGCGGTCAG 151 GACTACCGTC AGGΝGACGGC AAGTATTGAA GACGCCTTGA AATCAGACCC 201 TAAAAACGAG CTTGCCTGGC TGGTCCGTGC CGAAATCTAT CAATACCTGA 251 AAGTTAACGA CAAGGCGCAG GAAAGTTTCC GGCAAGΝCCT CTCCATCAAA 301 CCCGACAGTG CCGAAATCAA CAACAACTAC ΝGCTGGTTCC TGTGCGGCAG 351 GCTCAACCGC CCTGCCGAAT CTATGGCATA TTTCGACAAA GCCCTGGCCG 401 ACCCCACΝTA CCCGAΝCCCT TATATTGCCA ACCTGAATAA AGGCATATGC 451 AGCGCAAAAC AGGGGCAATT CGGATTGGCG GAAGCCTATT TGAAACGTTC 501 CCTCGCCGCC CAGCCGCAGT TCCCACCCGC ATTTAAAGAA CTGGCGCGCA 551 CCAAAATGCT GGCCGGGCAG TTGGGCGATG CCGATTACTA CTTTAAAAAA 601 TACCAAAGCA GGGTAGAAGT CCTTCAGGCC GATGATTTGC TGCTAGGCTG 651 GAAAATTGCC AAAGCCCTCG GCAACGCACA GGCGGCATAC GAATATGAAG 701 CACAATTGCA GGCGAATTTC CCCTACTCGG AAGAATTGCA AACCGTCCTC 751 ATCGGTCAAT AA This corresponds to the amino acid sequence <SEQ ID 2378; ORF 708. a>: a708.pep 1 MPFKPSKRIS LLLVLALGAC STSYRPSRAE KAΝQVSΝIKT QLAMEYMRGQ 51 DYRQXTASIE DALKSDPKNE LAWLVRAEIY QYLKVNDKAQ ESFRQXLSIK 101 PDSAEINNNY XWFLCGRLNR PAESMAYFDK ALADPTYPXP YIANLNKGIC 151 SAKQGQFGLA EAYLKRSLAA QPQFPPAFKE LARTKMLAGQ LGDADYYFKK 201 YQSRVEVLQA DDLLLGWKIA KALGNAQAAY EYEAQLQANF PYSEELQTVL 251 IGQ* a708/m708 98.0% identity in 253 aa overlap 10 20 30 40 50 60 a708.pep MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAMEYMRGQDYRQXTASIE II II II II I II II II II I I II II II II I I II II I I I I II II II II II II II I II II II I m708 MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAMEYMRGQDYRQATASIE 10 20 30 40 50 60 70 80 90 100 110 120 a708.pep DALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQXLSIKPDSAEINNNYXWFLCGRLNR II I II I II II II II II I II II I II II II II II II I llllllllllllll lllllllll m708 DALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPDSAEINNNYGWFLCGRLNR 70 80 90 100 110 120 130 140 150 160 170 180 a708.pep PAESMAYFDKALADPTYPXPYIANLNKGICSAKQGQFGLAEAYLKRSLAAQPQFPPAFKE II II II II I II II II II I: I II II I II I II II I I I II II I II I II II II II II I II II II m708 PAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLAEAYLKRSLAAQPQFPPAFKE 130 140 150 160 170 180 190 200 210 220 230 240 a708. pep LARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWKIAKALGNAQAAYEYEAQLQANF II II I II II II II II I II II II I II I II II I II I I II I II I II II II II II II II II II I 708 LARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWKIAKALGNAQAAYEYEAQLQANF 190 200 210 220 230 240 250 a708.pep PYSEELQTVLIGQX II II II II II III m708 PYSEELQTVLTGQX 250 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2379>: g709. .seq 1 ATGTTTGCTT TCAAATCCTT ACTCGATATG CCGCGCGGTG AGGCACTTGC 51 CGTCGTCGTC GCTCTGATTG CCGCAATGGG CTATACCATC ATTTCATTGG 101 AGTGGCTGCC GCATATGTCC ATTATTGCCG CCATCGTCGT GCTGATTTTG 151 TACGGCTTGG CGCGCGGTTT GAAATACAAC GATATGCAGG CAGGGATGAT 201 AGGCGCGTTG AATCAGGGTA TGGGCGCGGT TTACCTGTTT TTCTTCATCG 251 GGCTGATGGT CAGCGCGCTG ATGATGAGCG GCGCGATTCC GACGCTGATG 301 TATTACGGTT TCGGGCTGAT TTCCCCGACT TATTTTTATT TTTCCGCCTT 351 CGCGCTGTGT TCCGTCATCG GCGTGTCCAT CGGCAGCAGC CTGACCGCCT 401 GCGCCACTGT CGGCGTTGCC TTTATGGGGA TGGCGGCGGC GTTTCAGGCC 451 GATATGGCGA TGACGgcggg cgcgattgTT tccggTGTGT TTTTCGGCGA 501 TAAAATGTCC CCGCTTTCCG ACACCACGGG CATTTCCGCG TCCATCGTCG 551 GTATCGACCT GTTTGAACAC ATCAAAAACA TGATGTACAC CACCATCCCT 601 GCGTGGCTTA TCAGCGCGGC ACTGATGCTT TGGCTTCTTC CCAGCGTCGC 651 CGCGCAGGAT TTGAACAGCG TCGAATCCTT CCGCAGCCAG CTTGAAGCCA 701 CGGGATTGGT GCACGGCTAT TCGCTGATTC CGTTTGCACT GTTGGTCGTT 751 TTGGCATTGA TGCGCGTCAA TGCCGTGGTC GCCATGCTCT TTACCGTCAT 801 TGCCGCCGTT GCCGTAACGT ATCTGCACAG CACGCCCGAT CTGCGTCAGC 851 TCGGCGCGTG GTTTTATGGC GGCTACAAAC TCGAAGGCGA AGCGTTTAAA 901 GACATTGCCA AACTGATTTC GCGCGGCGGC TTGGAGAGTA TGTTCTTTAC 951 GCAGACCATC GTTATCCTCG GTATGAGTTT GGGCGGGCTG CTGTTTGCGC 1001 TCGGTGTGAT TCCTTCCTTG CTGGAGGCCG TCCGTACCTT CTTGACGAAT 1051 GCCGGACGCG CGACGTTCAG CGTTGCCATG ACTTCGGTCG GGGTCAATTT 1101 CCTGATTGGA GAGCAATATT TGAGCATCCT GCTTTCGGGA GAAACGTTCA 1151 AACCCGTTTA CGACAAACTC GGCCTGCATT CGTGCAACCT GTCGCGGACT 1201 CTGGAAGATG CGGGGACGGT GATTAACCCG CTCGTGCCGT GGAGCGTGTG 1251 CGGCGTATTT ATCAGCCACG CCCTTGGCGT ACCCGTTTGG GAATATCTGC 1301 CTTATGCCTT TTTCTGCTAT TTGAGTTTGG CTTTAACCCT GTTATTCGGC 1351 TGGACGGGGC TGACTTTGAG CAAAAAATAA This corresponds to the amino acid sequence <SEQ ID 2380; ORF 709.ng>: g709.pep 1 MFAFKSLLDM PRGEALAVVV ALIAAMGYTI ISLEWLPHMS IIAAIVVLIL 51 YGLARGLKYΝ DMQAGMIGAL ΝQGMGAVYLF FFIGLMVΞAL MMSGAIPTLM 101 YYGFGLISPT YFYFSAFALC SVIGVΞIGSS LTACATVGVA FMGMAAAFQA 151 DMAMTAGAIV SGVFFGDKMS PLSDTTGIΞA ΞIVGIDLFEH IKΝMMYTTIP 201 AWLISAALML WLLPSVAAQD LΝΞVESFRΞQ LEATGLVHGY ΞLIPFALLVV 251 LALMRVΝAVV AMLFTVIAAV AVTYLHΞTPD LRQLGAWFYG GYKLEGEAFK 301 DIAKLISRGG LEΞMFFTQTI VILGMΞLGGL LFALGVIPSL LEAVRTFLTΝ 351 AGRATFSVAM TSVGVNFLIG EQYLΞILLΞG ETFKPVYDKL GLHSCNLSRT 401 LEDAGTVINP LVPWSVCGVF ISHALGVPVW EYLPYAFFCY LSLALTLLFG 451 WTGLTLSKK* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2381>: m709.seq 1 ATGTTCGCTT TCAAATCCTT ACTCGATATG CCGCGCGGTG AGGCACTTGC 51 CGTCGTCGTC GCTCTGATTG CCGCGATGGG CTATACCATC ATTTCATTGG 101 AGTGGTTGCC GCATATGTCC ATTATTGCCG CCATCGTCGT GCTGATTTTG 151 TACGGCTTGG CGCGCGGTTT GAAATACAAC GATATGCAGC AGGGCATGAT 201 AGGCGCGTTG AATCAGGGTA TGGGCGCGAT TTACCTGTTT TTCTTCATCG 251 GGCTGATGGT CAGCGCGCTG ATGATGAGCG GCGCGATTCC GACGCTGATG 301 TATTACGGTT TCGGACTGAT TTCCCCGACT TATTTTTATT TTTCCTCCTT 351 CGCGCTGTGT TCCGTCATCG GCGTGTCCAT CGGCAGCAGC CTGACCACCT 401 GCGCCACTGT CGGCGTTGCC TTTATGGGGA TGGCGGCGGC GTTTCAGGCC 451 GATATGGCGA TGACGGCGGG CGCGATTGTT TCGGGCGCAT TTTTTGGCGA 501 CAAAATGTCC CCGCTTTCGG ATACGACGGG TATTTCCGCG TCCATCGTCG 551 GCATCGACTT GTTTGAGCAC ATCAAAAATA TGATGTACAC CACCATCCCC 601 GCGTGGCTCA TTAGTGCGGC ACTGATGCTT TGGCTTTTGC CGAATGTCGC 651 CGCGCAGGAT TTGAACAGCG TCGAATCCTT CCGCAGCCAG CTTGAAGCCA 701 CGGGATTGGT GCACGGCTAT TCGCTGATTC CGTTTGCGCT GTTGGTCATT 751 TTGGCATTGA TGCGCATCAA CGCCGTCGTC GCCATGCTCT TTACCGTCAT 801 GGTTGCCGTT GCTGTAACGT ATCTGCACAG CACGCCCGAT CTGCGTCAGC 851 TCGGTGCGTG GTTTTACGGC GGCTACAAAC TCGAAGGCGA AGCGTTTAAA 901 GATGTTGTCA AACTGATTTC GCGCGGCGGT TTGGAAAGTA TGTTTTTCAC 951 GCAAACCATC GTGATTCTCG GGATGAGTTT GGGCGGACTG TTGTTTGCGC 1001 TCGGTGTGAT TCCTTCCCTG TTGGAGGCCA TCCGTACCTT CTTGACGAAT 1051 GCCGGACGCG CGACGTTCAG CGTTGCCATG ACTTCGGTCG GGGTTAATTT 1101 CCTGATCGGC GAGCAATATT TGAGTATTTT GTTGTCGGGT GAAACGTTCA 1151 AACCCGTTTA CGATAAGCTC GGTCTGCATT CGCGCAATCT GTCGCGGACG 1201 CTGGAAGATG CGGGGACGGT GATTAACCCG CTCGTACCGT GGAGCGTATG 1251 CGGCGTGTTC ATCAGCCACG CGCTGGGCGT GCCGGTTTGG GAATATCTGC 1301 CGTATGCCTT TTTCTGCTAT TTGAGTTTGG CTTTGACCCT GTTATTCGGT 1351 TGGACGGGGC TGACTTTGAG CAAAAAATAA This corresponds to the amino acid sequence <SEQ ID 2382; ORF 709>: 709.pep 1 MFAFKSLLDM PRGEALAVVV ALIAAMGYTI ISLEWLPHMS IIAAIVVLIL 51 YGLARGLKYΝ DMQQGMIGAL ΝQGMGAIYLF FFIGLMVSAL MMSGAIPTLM 1 10011 YYGFGLISPT YFYFSSFALC SVIGVSIGSΞ LTTCATVGVA FMGMAAAFQA 1 15511 DMAMTAGAIV SGAFFGDKMS PLSDTTGISA ΞIVGIDLFEH IKNMMYTTIP 201 AWLISAALML WLLPNVAAQD LNSVESFRSQ LEATGLVHGY SLIPFALLVI 251 LALMRIΝAVV AMLFTVMVAV AVTYLHΞTPD LRQLGAWFYG GYKLEGEAFK 301 DVVKLISRGG LEΞMFFTQTI VILGMSLGGL LFALGVIPSL LEAIRTFLTN 351 AGRATFSVAM TSVGVNFLIG EQYLΞILLSG ETFKPVYDKL GLHSRNLSRT 401 LEDAGTVINP LVPWSVCGVF ISHALGVPVW EYLPYAFFCY LSLALTLLFG 451 WTGLTLSKK* m709/g709 96.9% identity in 459 aa overlap 10 20 30 40 50 60 m709.pep MFAFKΞLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMΞIIAAIVVLILYGLARGLKYN I I I I I I I I I I I I II II II I I I I I I I I I I I I I I I II I I I I I II I I I I I I I I I I I I I I I I I I g709 MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAAIVVLILYGLARGLKYN 10 20 30 40 50 60 70 80 90 100 110 120 m709.pep DMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYYGFGLIΞPTYFYFSSFALC III I I I I I I I I I I I Ml I I I I I II I II I I I I I I I I II I I I I I I I I I I I I I I I I Ml I I I g709 DMQAGMIGALNQGMGAVYLFFFIGLMVΞALMMΞGAIPTLMYYGFGLISPTYFYFΞAFALC 70 80 90 100 110 120 130 140 150 160 170 180 m709.pep SVIGVSIGSSLTTCATVGVAFMGMAAAFQADMAMTAGAIVΞGAFFGDKMSPLΞDTTGISA I II I I I I I I I I l I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I g709 SVIGVSIGΞΞLTACATVGVAFMGMAAAFQADMAMTAGAIVSGVFFGDKMSPLSDTTGISA 130 140 150 160 170 180 190 200 210 220 230 240 m709.pep SIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPNVAAQDLNSVESFRΞQLEATGLVHGY I I I I I I I I I I I I I I I I I M I II III I II I I I I I l II I I I I II I I I I I I I I I I I I I I I I g709 SIVGIDLFEHIKNMMYTTIPAWLIΞAALMLWLLPSVAAQDLNSVESFRSQLEATGLVHGY 190 200 210 220 230 240 250 260 270 280 290 300 m709. pep SLIPFALLVILALMRINAVVAMLFTVMVAVAVTYLHΞTPDLRQLGAWFYGGYKLEGEAFK I II I I I I I Ml I I I I : I I I I I I I I I MM I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I g709 SLIPFALLVVLALMRVNAVVAMLFTVIAAVAVTYLHΞTPDLRQLGAWFYGGYKLEGEAFK 250 260 270 280 290 300 310 320 330 340 350 360 m709. pep DVVKLIΞRGGLESMFFTQTIVILGMSLGGLLFALGVIPΞLLEAIRTFLTNAGRATFSVAM M: I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I Ml I I I I I I I I I I I I I I I g709 DIAKLISRGGLESMFFTQTIVILGMSLGGLLFALGVIPΞLLEAVRTFLTNAGRATFSVAM 310 320 330 340 350 360 370 380 390 400 410 420 m709. .pep TSVGVNFLIGEQYLSILLSGETFKPVYDKLGLHSRNLSRTLEDAGTVINPLVPWSVCGVF I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g709 TSVGVNFLIGEQYLΞILLSGETFKPVYDKLGLHSCNLΞRTLEDAGTVINPLVPWSVCGVF 370 380 390 400 410 420 430 440 450 460 m709. •pep ISHALGVPVWEYLPYAFFCYLΞLALTLLFGWTGLTLSKKX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g709 ISHALGVPVWEYLPYAFFCYLΞLALTLLFGWTGLTLSKKX 430 440 450 460 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2383>: a709.seq 1 ATGTTCGCTT TCNAATCCTT ACTCGATATG CCGCGCGGTG AGGCNCTTGC 51 CGTCGTCGTC GCTCTGATTG CCGCGATGGG CTATACCATC ATTTNNTTGG 101 AGTGGCTGCC GCATATGTCC ATTATTGCCG CCATCGTΝGT GCTGATTTTG 151 TACGGCTTGG CGCGCGGTTT GAAATACAAC GATATGCAGC AGGGCATGAT 201 AGGCGCGTTG AATCAGGGTA TGGGCGCGAT TTACCTΝTTT TTCTTCATCG 251 GGCTGATGGT CAGCGCGCTG ATGATGAGCG GCGCGATTCC GACGCTGATG 301 TATTACGGTT TCGGACTGAT TTCCCCGACT TATTTTTATT TTTCCGCCTT 351 CGCGCTGTGT TCCGTCATCG GCGTGTCCAT CGGCAGCAGC CTGACCACCT 401 GCGCCACTGT CGGCGTTGCC TTΝATGGGTA TΝΝΝGΝCGGC GTTTCNGGCC 451 NANATGGNGN NGNNGNNGGN CΝΝGATTGTΝ ΝΝGGΝCGCAT TNTTNGGCGN 501 CAAAATGTCN CCGCTTTCCG ATACGΝCGGG CATΝTCCGCG TCCATTGTCG 551 GTATCGACCT GTTTGAACAC ATCAAAAATA TGATGTACAC NACCATTCCC 601 GCGTGGCTCA TCAGTGNNNC ACTGATGCTG TΝGCTTCTTC CCAGCGTCGC 651 CGCGCAGGAT TTGAACAGCG TCGAATCCTT CCGCAGCCAG CTTGAAGCCA 701 CGGGATTGGT GCACTGCTAT TCGCTGATTC CGTTTGCGCT GTTGGTCGTT 751 TTGGCATTGA TGCGCGTCAA TGCCGTGGTC GCTATGCTCT TTACCGTCAT 801 TGCCGCCGTT GCCGTAACGT ATCTGCACAG CACGCCCGAT CTGCGTCAGC 851 TCGGCGCGTG GTTTTACGGC GGCTACAAAC TCGAAGGCGA AGCGTNTANA 901 GACATTGCCA AACTCATTTC TCGCGGCGGT TTGGAAAGTA TGTTTTTCAC 951 GCAGACCATC GTGATTCTTG GGATGAGCCT TGGCGGGCTG CTGTTTGCAC 1001 TGGGCGCGAT TCCTTCCCTG CTGGATGCCG TCCGCAGCTT TTTGACGAAT 1051 GCCGGGCGTN CCACATTCAG CGTTGCCATG ACTTCGGTCG GGGTTAATTT 1101 CCTGATCGGC GAGCAATATT TGAGTATTTT GTTGTCNGGT GAAACGTTCA 1151 AACCTGTTTA CGATAAGCTC GGTCTGCATT CGCGCAATCT GTCGCGGACG 1201 CTGGAAGATG CGGGGACGGT CATCAACCCG CTCGTACCGT GGAGCGTATG 1251 CGGCGTGTTC ATCANCCACG CGCTGGGCGT GCCGGTTTGG GAATATCTGC 1301 CGTATGCCTT TTTCTGCTAT TTGAGTTTGG CTTTGACCCT GTTATTCGGT 1351 TGGACGGGGC TGACTTTGAG CAAAAAATAA This corresponds to the amino acid sequence <SEQ ID 2384; ORF 709.a>: a709.pep 1 MFAFXSLLDM PRGEALAVVV ALIAAMGYTI IXLEWLPHMS IIAAIVVLIL 51 YGLARGLKYN DMQQGMIGAL NQGMGAIYLF FFIGLMVSAL JWfSGAIPTLM 101 YYGFGLISPT YFYFSAFALC SVIGVSIGSS LTTCATVGVA XMGXXXAFXA 151 XMXXXXXXIV XXAXXGXKMS PLSDTXGXSA SIVGIDLFEH IKNMMYTTIP 201 AWLISXXLML XLLPSVAAQD LNSVESFRSQ LEATGLVHCY SLIPFALLVV 251 LALMRVNAW AMLFTVIAAV AVTYLHSTPD LRQLGAWFYG GYKLEGEAXX 301 DIAKLISRGG LESMFFTQTI VILGMSLGGL LFALGAIPSL LDAVRSFLTN 351 AGRXTFSVAM TSVGVNFLIG EQYLSILLSG ETFKPVYDKL GLHSRNLSRT 401 LEDAGTVINP LVPWSVCGVF IXHALGVPVW EYLPYAFFCY LSLALTLLFG 451 WTGLTLSKK* a709/m709 91.1% identity in 459 aa overlap 10 20 30 40 50 60 a709. pep MFAFXSLLDMPRGEALAWVALIAAMGYTIIXLEWLPHMSIIAAIWLILYGLARGLKYN II II I I I II II II II II II II II II I II II I II II II II II II II I II I II II II I II m709 MFAFKSLLDMPRGEALAVWALIAAMGYTIISLEWLPHMSIIAAIWLILYGLARGLKYN 10 20 30 40 50 60 70 80 90 100 110 120 a709.pep DMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYYGFGLISPTYFYFSAFALC I II II I II I I I II I II II II I II II II II II II II I II I II II I II II II I I II I : II II m709 DMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYYGFGLISPTYFYFSSFALC 70 80 90 100 110 120 130 140 150 160 170 180 a709. pep SVIGVSIGSSLTTCATVGVAXMGXXXAFXAXMXXXXXXIVXXAXXGXKMSPLSDTXGXSA I II II II I I II II II I II II II ll l l : II I I II I I II IM I II m709 SVIGVSIGSSLTTCATVGVAFMGMAAAFQADMAMTAGAIVSGAFFGDKMSPLSDTTGISA 130 140 150 160 170 180 190 200 210 220 230 240 a709.pep SIVGIDLFEHIKNMMYTTIPAWLISXXLMLXLLPSVAAQDLNSVESFRSQLEATGLVHCY II II II II II II II II II I II II I I II I II I : II II I II II II II II I II II II I I m709 SIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPNVAAQDLNSVESFRSQLEATGLVHGY 190 200 210 220 230 240 250 260 270 280 290 300 a709. pep SLIPFALLWLALMRVNAWAMLFTVIAAVAVTYLHSTPDLRQLGAWFYGGYKLEGEAXX I II II I II I : II II I : II II II II II : : I I II II I II II II I II II II II I II II II I m709 SLIPFALLVILALMRINAWAMLFTVMVAVAVTYLHSTPDLRQLGAWFYGGYKLEGEAFK 250 260 270 280 290 300 310 320 330 340 350 360 a709. pep DIAKLISRGGLESMFFTQTIVILGMSLGGLLFALGAIPSLLDAVRSFLTNAGRXTFSVAM I :: II II I II II II II I II II II I II II II II II I : I II I I : I : I : II I II I I m709 DWKLISRGGLESMFFTQTIVILGMSLGGLLFALGVIPSLLEAIRTFLTNAGRATFSVAM 310 320 330 340 350 360 370 380 390 400 410 420 a709.pep TSVGVNFLIGEQYLSILLΞGETFKPVYDKLGLHSRNLSRTLEDAGTVINPLVPWSVCGVF I II I II II I II I I I II II II II I II II II I II I II II II II II I I II II II I II II II II m709 TSVGVNFLIGEQYLSILLSGETFKPVYDKLGLHSRNLSRTLEDAGTVINPLVPWSVCGVF 370 380 390 400 410 420 430 440 450 460 a709. pep IXHALGVPVWEYLPYAFFCYLSLALTLLFGWTGLTLSKKX I II I I I I I I II II II II II II II II I II II II I I II II I m709 ISHALGVPVWEYLPYAFFCYLSLALTLLFGWTGLTLSKKX 430 440 450 460 g710.seq not found g710.pep not found The following partial DNA sequence was identified in N meningitidis <SEQ LD 2385>: m710. seq 1 ATGGAAACCC ACGAAAAAAT CCGCCTGATG CGCGAATTGA ATAAATGGTC 51 CCAGGAGGAT ATGGCGGAAA AGCTGGCGAT GTCGGCAGGC GGGTATGCCA 101 AAATCGAACG GGGCGAAACG CAGTTAAATA TCCCGCGTTT GGAGCAGTTG 151 GCTCAGATTT TCAAAATCGA TATGTGGGAC TTGCTCAAAT CGGGCGGTGG 201 TGGGATGGTG TTTCAGATTA ATGAAGGTGA TAGTGGTGGC GATATTGCGT 251 TGTATGCGTC GGGTGATGTT TCGATGAAAA TAGAATTTTT AAAAATGGAG 301 TTGAAACACT GCAAAGAAAT GTTGGAACAA AAAGACAAAG AAATCGAGCT 351 GCTCCGCAAG CTGACCGAAA CCGTTTAA This corresponds to the amino acid sequence <SEQ ID 2386; ORF 710>: m710. pep 1 METHEKIRLM RELNKWSQED MAEKLAMSAG GYAKIERGET QLNIPRLEQL 51 AQIFKIDMWD LLKSGGGGMV FQINEGDSGG DIALYASGDV SMKIEFLKME 101 LKHCKEMLEQ KDKEIELLRK LTETV* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2387>: a710.seq 1 ATGGAAACCC ACGAAAAAAT CCGCCTGATG CGCGAATTGA ATAAATGGTC 51 CCAGGAGGAT ATGGCGGAAA AGCTGGCGAT GTCGGCAGGC GGGTATGCCA 101 AAATCGAACG AGGCGAAACG CAGTTGAATA TCCCGCGTTT GGAGCAGTTG 151 GCGCAGATTT TCAAAATTGA TATGTGGGAC TTGCTCAAAT CGGGCGGCGG 201 CGGGATGGTG TTGCAGATTA ACGATGTGGA TACCAACAGC GGGGAATTTG 251 CAATCTATAC CGCTCAGGAT GCATCΝGGTA AAGCTGGATT TGTTAAAATG 301 GAATTAAAAC ACTGTAAAGA AATGTTGGAA CACAAAGACA AAGAAATCGA 351 GCTGCTCCGC AAGCTGACCG AAACCGTTTA A This corresponds to the amino acid sequence <SEQ ID 2388; ORF 710.a>: a710.pep 1 METHEKIRLM RELNKWSQED MAEKLAMSAG GYAKIERGET QLNIPRLEQL 51 AQIFKIDMWD LLKSGGGGMV LQINDVDTNS GEFAIYTAQD ASGKAGFVKM 101 ELKHCKEMLE HKDKEIELLR KLTETV* a710/m710 85.7% identity in 126 aa overlap 10 20 30 40 50 60 a710.pep METHEKIRLMRELNKWSQEDMAEKLAMSAGGYAKIERGETQLNIPRLEQLAQIFKIDMWD I II II II I I II II II II II II I II I II I I II II I I I I I II I II II II II II I I II II I I I m710 METHEKIRLMRELNKWSQEDMAEKLAMSAGGYAKIERGETQLNIPRLEQLAQIFKIDMWD 10 20 30 40 50 60 70 80 90 100 110 120 a710. pep LLKSGGGGMVLQINDVDTNSGEFAIYTAQDASGKAGFVKMELKHCKEMLEHKDKEIELLR M M M M I M M M M : M M M : : M l I I : I I I I I I I I I I I I M I I I I I I I I m710 LLKSGGGGMVFQINEGDSG-GDIALYASGDVSMKIEFLKMELKHCKEMLEQKDKEIELLR 70 80 90 100 110 a710.pep KLTETVX MIIIM m710 KLTETVX 120 g711.seq not found g711.pep not found The following partial DNA sequence was identified in N meningitidis <SEQ ID 2389>: m711. seq 1 ATGCCCGCGC CTGATTTGGG ATTTGCCTTA AGTCTGCCGC CAAAAAAGGC 51 AATCGAGTGG CTGGAAAGTA AAAAGGTTAC GGCGGAGAGC TACCGCAATC 101 TGACAGCCTC CGAAATTGCC AAAGTCTATA CGATTGCCCG CATGACCGAC 151 TTGGATATGC TCAACGACAT CAAAACTTCG ATGGTTGAAT CGGCAAAAAG 201 TGGACAGTCG TTTGACGATT GGCGAAAAGG TATCTTGAAT CTGCTCAGCA 251 ACAAGGGCTG GCTGCATCCG AACGGGCATA ACGGTAAGGA TATCATCGAC 301 CCAGCCACCG GCGAGGTATT CGGTTCGCCG CGGAGGTTGG AGACGATTTA 351 CCGTACCAAT ATGCAAACTG CCTACAACGC CGGTCAATAT CAAGGATATA 401 TGGCAAATAT TGATGCACGA CCTTATTGGA TGTATGACGC GG AGGCGAC 451 AGCCGCACCC GTCCGGCGCA TTCGGCAATA GACGGGCTGG TGTACCGCTA 501 CGACGACCCG TTTTGGGCAA CGTTTTACCC GCCCAACGGC TACAACTGCC 551 GCTGCTCGGT CATCGCGCTG TCGGAGCGGG ATGTGGAACG CCAGGGGCGG 601 ATTGTTGGGC AAAGCACGGC GGACAATCTG GTCGAGACCC ATAAAATCTA 651 CAACAAAAAA GGCGATACTT ATCTGACCCT TGCCTATAAA GCACCGGATG 701 GCAGTCTGTA CACGACCGAT CGAGGATTTG ATTACAACGC CGGACGAATG 751 AACTACCGCC CCGATTTAGA CAAGTACGAC CGTGCGTTGG CGCATCAATT 801 TGCCAAAGCG GAAATGGGTG GTGCGGATTT TAAAACCAGC TTTAAACAGC 851 TTGAAAAAGA GTTTTATGAA GTCAAGCAAC GTTTGGATAT TGATGGCAAG 901 CCCGATAAAG AGCAGAAAAT CAAAATCCGA AATGCGCTAT CAAGACAGCT 951 TAAATTTGCT GCGGGTGTAT TGAGCAAGGA AACGCAAGAA TTGGCAGGTA 1001 TGACACGAGC GACGGTGTGG CTGTCTGATG ATACGTTGGT TAAACAGGTA 1051 GACAGCCGTG AGGGGCAGAA TTTCGATGAC TCCTACTATG CTTTTTTGCC 1101 GGATATGCTG CAAAACCCTG AACATGTCAT CCGCGACAAT CGTGAATTGA 1151 TTTTCACAGC TCGCTATAAA GGCTCGGCAT TGTGGGCAGT TTTAAAATAT 1201 ATTAAGGAGG TGGATGAGAT TTATCTACAG TCGTACCGAA TCAGTAACGA 1251 CAAAGAGATT GCCAAATTTA TGGCGAAGAA GAAAGTATTG AAATAG This corresponds to the amino acid sequence <SEQ ID 2390; ORF 711>: m711.pep 1 MPAPDLGFAL SLPPKKAIEW LESKKVTAES YRΝLTASEIA KVYTIARMTD 51 LDMLΝDIKTS MVESAKSGQS FDDWRKGILΝ LLSΝKGWLHP ΝGHΝGKDIID 101 PATGEVFGSP RRLETIYRTΝ MQTAYΝAGQY QGYMAΝIDAR PYWMYDAVGD 151 SRTRPAHSAI DGLVYRYDDP FWATFYPPΝG YΝCRCSVIAL SERDVERQGR 201 IVGQSTADΝL VETHKIYΝKK GDTYLTLAYK APDGSLYTTD RGFDYΝAGRM 251 ΝYRPDLDKYD RALAHQFAKA EMGGADFKTS FKQLEKEFYE VKQRLDIDGK 301 PDKEQKIKIR ΝALSRQLKFA AGVLSKETQE LAGMTRATVW LSDDTLVKQV 351 DSREGQΝFDD SYYAFLPDML QΝPEHVIRDΝ RELIFTARYK GSALWAVLKY 401 IKEVDEIYLQ SYRISΝDKEI AKFMAKKKVL K* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2391 >: a711. seq 1 ATGCCCGCGC CTGATTTGGG ATTTGCCTTA AGTCTGCCGC CAAAAAAGGC 51 AATCGAGTGG CTGGAAAGTA AAAAGGTTAC GGCGGAGAGC TACCGCAATC 101 TGACAGCCTC CGAAATTGCC AAAGTCTATA CGATTGCCCG CATGACCGAC 151 TTGGATATGC TCAACGACAT CAAAACTTCG ATGGTTGAAT CGGCAAAAAG 201 TGGACAGTCG TTTGACGATT GGCGAAAAGG TATCTTGAAT CTGCTCAGCA 251 ACAAGGGCTG GCTGCATCCG AACGGGCATA ACGGTAAGGA TATCATCGAC 301 CCAGCCACCG GCGAGGTATT CGGTTCGCCG CGGAGGTTGG AGACGATTTA 351 CCGTACCAAC ATGCAAACTG CCTACAACGC CGGTCAATAT CAAGGATATA 401 TGGCAAATAT TGATGCACGA CCTTATTGGA TGTATGACGC GGTAGGCGAC 451 AGCCGCACCC GTCCGGCGCA TTCGGCAATA GACGGGCTGG TGTACCGCTA 501 CGACGACCCG TTTTGGGCAA CGTTTTACCC GCCCAACGGC TACAACTGCC 551 GTTGCTCGGT CATCGCGCTG TCGGAGCGGG ATGTGGAACG CCAGGGGCGG 601 ATTGTCGGGC AAAGCACGTC GGACAATCTT GTTGAGACCC ATAAAATCTA 651 CAACAAAAAA GGCGATACTT ATCTGACCCT TGCCTATAAA GCACCGGATG 701 GCAGTCTGTA CACGACCGAT CGAGGATTTG ATTACAACGC CGGACGAATG 751 AACTACCGCC CCGATTTAGA CAAGTACGAC CGTGCGTTGG CGCATCAATT 801 TGCCAAAGCG GAAATGGGTG GTGCGGATTT TAAAACCAGC TTTAAACAGC 851 TTGAAAAAGA GTTTTATGAA GTCAAGCAAC GTTTGGATAT TGATGGCAAG 901 CCCGATAAAG AGCAGAAAAT CAAAATCCGA AATGCGCTAT CAAGACAGCT 951 TAAATTTGCT GCGGGTGTAT TGAGCAAGGA AACGCAAGAA TTGGCAGGTA 1001 TGACACGAGC GACGGTGTGG CTGTCTGATG ATACGTTGGT TAAACAGGTA 1051 GACAGCCGTG AAGGGCAGAA TTTCGATGAC TCCTACTATG CTTTTTTGCC 1101 GGATATGCTG CAAAACCCTG AACATGTCAT CCGCGACAAT CGTGAATTGA 1151 TTTTCACAGC TCGCTATAAA GGCTCGGCAT TGTGGGCAGT TTTAAAATAT 1201 ATTAAGGAGG TGGATGAGAT TTATCTACAG TCGTACCGAA TCAGTAACGA 1251 CAAAGAGATT GCCAAATTTA TGGCGAAGAA GAAAGTATTG AAATAG This corresponds to the amino acid sequence <SEQ ID 2392; ORF 71 l.a>: a711.pep 1 MPAPDLGFAL SLPPKKAIEW LESKKVTAES YRNLTASEIA KVYTIARMTD 51 LDMLNDIKTS MVESAKSGQS FDDWRKGILN LLSNKGWLHP NGHNGKDIID 101 PATGEVFGSP RRLETIYRTN MQTAYNAGQY QGYMANIDAR PYWMYDAVGD 151 SRTRPAHSAI DGLVYRYDDP FWATFYPPNG YNCRCSVIAL SERDVERQGR 201 IVGQSTSDNL VETHKIYNKK GDTYLTLAYK APDGSLYTTD RGFDYNAGRM 251 NYRPDLDKYD RALAHQFAKA EMGGADFKTS FKQLEKEFYE VKQRLDIDGK 301 PDKEQKIKIR NALSRQLKFA AGVLSKETQE LAGMTRATVW LSDDTLVKQV 351 DSREGQNFDD SYYAFLPDML QNPEHVIRDN RELIFTARYK GSALWAVLKY 401 IKEVDEIYLQ SYRISNDKEI AKFMAKKKVL K* all l/m711 99.8% identity in 431 aa overlap 10 20 30 40 50 60 a711.pep MPAPDLGFALSLPPKKAIEWLESKKVTAESYRNLTASEIAKVYTIARMTDLDMLNDIKTS II II II II II II II I II II II I I II II I II II II II II II II II II II I II II II II I II m711 MPAPDLGFALSLPPKKAIEWLESKKVTAESYRNLTASEIAKVYTIARMTDLDMLNDIKTS 10 20 30 40 50 60 70 80 90 100 110 120 a711.pep MVESAKSGQSFDDWRKGILNLLSNKGWLHPNGHNGKDIIDPATGEVFGSPRRLETIYRTN I II II II II II II II II II II II II II I II I II II I II II I II II I II II II I II II I II m711 MVESAKSGQSFDDWRKGILNLLSNKGWLHPNGHNGKDIIDPATGEVFGSPRRLETIYRTN 70 80 90 100 110 120 130 140 150 160 170 180 a711. pep MQTAYNAGQYQGYMANIDARPYWMYDAVGDSRTRPAHSAIDGLVYRYDDPFWATFYPPNG I II I II II II II I II I I II II I I II I II II I II I II I II I II II II II II II II II I I II m711 MQTAYNAGQYQGYMANIDARPYWMYDAVGDSRTRPAHSAIDGLVYRYDDPFWATFYPPNG 130 140 150 160 170 180 190 200 210 220 230 240 a711.pep YNCRCSVIALSERDVERQGRIVGQSTSDNLVETHKIYNKKGDTYLTLAYKAPDGSLYTTD I I II II II II II II I II II II II II M I II II I I II I II I II II II I I I II II II II II I m711 YNCRCSVIALSERDVERQGRIVGQSTADNLVETHKIYNKKGDTYLTLAYKAPDGSLYTTD 190 200 210 220 230 240 250 260 270 280 290 300 a711. pep RGFDYNAGRMNYRPDLDKYDRALAHQFAKAEMGGADFKTSFKQLEKEFYEVKQRLDIDGK II II II II II II II I II II I II I II I II II II II II I II II II II II I I II II II II II I m711 RGFDYNAGRMNYRPDLDKYDRALAHQFAKAEMGGADFKTSFKQLEKEFYEVKQRLDIDGK 250 260 270 280 290 300 310 320 330 340 350 360 a711.pep PDKEQKIKIRNALSRQLKFAAGVLSKETQELAGMTRATVWLSDDTLVKQVDSREGQNFDD II II II II II II I II I I II II II II II I II II I II II I I II II II II I I II II II II I I I m711 PDKEQKIKIRNALSRQLKFAAGVLSKETQELAGMTRATVWLSDDTLVKQVDSREGQNFDD 310 320 330 340 350 360 370 380 390 400 410 420 a711.pep SYYAFLPDMLQNPEHVIRDNRELIFTARYKGSALWAVLKYIKEVDEIYLQSYRISNDKEI II I II II I I I II II I II I II II I II I I I II II II I II II I II I II I II II II II I II I II m711 SYYAFLPDMLQNPEHVIRDNRELIFTARYKGSALWAVLKYIKEVDEIYLQSYRISNDKEI 370 380 390 400 410 420 430 a711.pep AKFMAKKKVLKX II II II II II II m711 AKFMAKKKVLKX 430 g712. seq not found yet g712.pep not found yet The following partial DNA sequence was identified in N meningitidis <SEQ ID 2393>: m712. seq 1 ATGATGCCCC ATATTGATTT TGACACGATT CCGGGCAGCA TCCGCGTGCC 51 CGGGCAGTAT ATTGAATTTA ACACCCGCAA TGCCGTACAA GGTTTGCCGC 101 AAAATCCGCA AAAGGTATTG ATGGTTGCAC CCATGCTGAC CGCGGGCATA 151 CAGCCCGCCT TAGAGCCGGT GCAACTATTT AGCGATGCCG AGGCGGCCGA 201 TTTGTTCGGA CAAGGCTCGC TGGCGCATTT GATGGTGCGC CAAGCATTTG 251 CCAACAACCC TTATTTGGAT TTGACCGTTA TCGGTATTGC CGACCACAGC 301 GCAGGCGTGC AGGCAACCGC AACCGTTACC CTTTCCGGCA CGGCCACCGC 351 GCCGGGCGTG GTGGAAATCA CGATTGGCGG CAAGCAGGTA AGCACGGCCG 401 TTAACACCGG CGAGACCGCC GCCACAGTGG CAGACCGTCT GAAAACCGCC 451 ATCACTGCCG CCGATGTAAC CGTTACCGCA TCCGGCAGCG GCGCAGCCGT 501 TACGCTGACG GCCAAACACA AAGGCGAGAT CGGCAACGAG AGCGGCTTAA 551 CCGTGAGCAC CGGCAATACC GGCCTAACTT ATCAAGCCAA TGCCTTTACC 601 GGCGGTGCCA AAAATGCGGA CATTGCCACG GCCTTGTCCA AAGTGGCGGG 651 CAAGCATTAT CACATTATTT GCAGCCCGTT TAGCGATGAC GCCAACGCCA 701 AAGCCTTGAG CAACCATATT ACCAACGTAT CCAACGCCAT CGAGCAGCGC 751 GGCTGTATCG GCGTATTGGG TATGAGTGCG GCCTTGAGCA CGGCCACCAC 801 CGCTACCGGC GAAATCAACG ACGGCCGCAT GACCTGTGCT TGGTACAAAG 851 GTGCGGTAGA GCCAAACGGC ATCATCGCCG CAGGTTATGC GGCGGTGTTG 901 GCCTTTGAAG AAGACCCTGC CAAGCCGCTG AACACGCTGG AAATCAAAGG 951 GCTGGCCGTT ACACCTGATG CGCAATGGCC GCTGTTTGCA GAATGCAACA 1001 ATGCGCTGTA CAACGGCTTG ACCCCGCTCA CAGTGGTCAA CAACCGCGTG 1051 CAGATTATGC GTGCCGTATC CACCTATACC AAGTCGGCCA ACAACACCGA 1101 CGACCCGGCA CTACTCGACA TTACCACCAT CCGCACGCTG GATTATGTGC 1151 GCCGCAGCGT TAAAGAGCGC ATTGCCCTGC GTTTTCCGCG CGACAAATTG 1201 AGCGACCGCC TGCTGCCCAA GGTTAAGAGC GAGATTTTGG ACGTGCTGAT 1251 TAAGCTCGAC CAAGCCGAAA TCATCGAAAA CGCCGAGGCC AACAAAGGCA 1301 AGCTGGTGGT GGCGCGTGCG CAAAACGACC CCAACCGTGT TAATGCCATT 1351 ATCCCCGCCG ATGTGGTCAA CGGCCTGCAC GTCTTTGCCG GGCGCATTGA 1401 TTTGATTTTG TAA This corresponds to the amino acid sequence <SEQ ID 2394; ORF 712>: m712.pep 1 MMPHIDFDTI PGSIRVPGQY IEFΝTRΝAVQ GLPQΝPQKVL MVAPMLTAGI 51 QPALEPVQLF SDAEAADLFG QGSLAHLMVR QAFAΝΝPYLD LTVIGIADHS 101 AGVQATATVT LSGTATAPGV VEITIGGKQV STAVΝTGETA ATVADRLKTA 151 ITAADVTVTA SGSGAAVTLT AKHKGEIGΝE SGLTVSTGΝT GLTYQAΝAFT 201 GGAKΝADIAT ALSKVAGKHY HIICSPFSDD AΝAKALSΝHI TΝVSΝAIEQR 251 GCIGVLGMSA ALSTATTATG EIΝDGRMTCA WYKGAVEPΝG IIAAGYAAVL 301 AFEEDPAKPL ΝTLEIKGLAV TPDAQWPLFA ECΝΝALYΝGL TPLTWΝΝRV 351 QIMRAVSTYT KSAΝΝTDDPA LLDITTIRTL DYVRRSVKER IALRFPRDKL 401 SDRLLPKVKS EILDVLIKLD QAEIIEΝAEA ΝKGKLWARA QΝDPΝRVΝAI 451 IPADWΝGLH VFAGRIDLIL * a712. seq not found yet a712.pep not found yet g713. seq not found yet g713.pep not found yet The following partial DNA sequence was identified in N meningitidis <SEQ ID 2395>: m713. seq 1 ATGCAAAATA ATTCATACGG CTATGCCGTG TCGGTGCGCG TGGGCGGTAA 51 AGAGCACCGC CACTGGGAGC GCTACGACAT CGACAGCGAC TTTTTAATCC 101 CTGCCGACAG CTTCGATTTT GTCATCGGCA GGTTGGGACC GGAGGCGGCC 151 ATACCCGATT TAAGCGGAGA GAGCTGCGAG GTAGTGATAG ACGGGCAAAT 201 CGTGATGACG GGCATCATCG GCAGCCAGCG CCACGGCAAA AGCAAGGGCA 251 GCCGCGAGTT GAGCTTGAGC GGGCGTGATT TGGCCGGTTT TTTGGTGGAT 301 TGCTCCGCGC CGCAGCTCAA TGTAAAGGGC ATGACGGTAT TGGATGCAGC 351 CAAAAAGCTG GCCGCGCCGT GGCCGCAGAT TAAAGCGGTG GTGCTTAAGG 401 CCGAAAACAA CCCCGCTTTG GGCAAAATCG ACATCGAGCC GGGCGAAACC 451 GTATGGCAGG CATTAACCCA TATTGCCAAC TCGGTCGGGC TGCATCCGTG 501 GCTGGAGCCG GACGGCACGT TGGTGGTGGG CGGTGCGGAT TACAGCAGCC 551 CGCCGGTGGC GACATTGTGT TGGAGCCGCA CCGACAGCCG CTGCAATATC 601 GAGCGCATGG ACATTGAGTG GGATACCGAC AACCGCTTTT CCGAGGTTAC 651 TTTTTTGGCG CAATCGCACG GCCGCAGCGG CGACAGCGCC AAACACGATT 701 TAAAGTGGGT GTACAAAGAC CCGACGATGA CGCTGCACCG CCCTAAAACG 751 GTGGTGGTGT CCGATGCCGA CAATTTGGCC GCATTGCAAA AGCAGGCTAA 801 AAAGCAGCTG GCCGACTGGC GGCTGGAGGG ATTTACACTC ACGATAACCG 851 TGGGCGGCCA TAAAACCCGC GACGGCGTAT TGTGGCAACC TGGCCTGCGT 901 GTGCATGTGA TCGACGACGA GCACGGTATC GATGCGGTGT TTTTTCTGAT 951 GGGGCGGCGG TTTATGCTAT CCCGCATGGA TGGTACGCAA ACCGAGCTGC 1001 GGCTCAAAGA GGACGGTATT TGGACACCCG ACGCTTACCC CAAAAAGGCC 1051 GAGGCGGCGC GCAAGCGCAA AGGCAAACGC AAAGGCGTGA GCCATAAGGG 1101 CAAAAAAGGC GGCAAAAAAC AAGCAGAAAC GGCGGTGTTT GAATGA This corresponds to the amino acid sequence <SEQ ID 2396; ORF 713>: m713.pep 1 MQΝΝSYGYAV SVRVGGKEHR HWERYDIDSD FLIPADSFDF VIGRLGPEAA 51 IPDLSGESCE WIDGQIVMT GIIGSQRHGK SKGSRELSLS GRDLAGFLVD 101 CSAPQLΝVKG MTVLDAAKKL AAPWPQIKAV VLKAEΝΝPAL GKIDIEPGET 151 VWQALTHIAΝ SVGLHPWLEP DGTLVVGGAD YSSPPVATLC WSRTDSRCΝI 201 ERMDIEWDTD ΝRFSEVTFLA QSHGRSGDSA KHDLKWVYKD PTMTLHRPKT 251 WVSDADΝLA ALQKQAKKQL ADWRLEGFTL TITVGGHKTR DGVLWQPGLR 301 VHVIDDEHGI DAVFFLMGRR FMLSRMDGTQ TELRLKEDGI WTPDAYPKKA 351 EAARKRKGKR KGVSHKGKKG GKKQAETAVF E* The following partial DΝA sequence was identified inN meningitidis <SEQ ID 2397>: a713.seq 1 ATGCAAAATA ATTCATACGG CTATGCCGTG TCGGTGCGCG TGGGCGGTAA 51 AGAGCACCGC CACTGGGAGC GCTACGACAT CGACAGCGAC TTTTTAATCC 101 CTGCCGACAG CTTCGATTTT GTCATCGGCA GGTTGGGGCC GGAGGCGGCC 151 ATACCCGATT TAAGCGGAGA GAGCTGCGAG GTAGTGATAG ACGGGCAAAT 201 CGTGATGACG GGCATCATCG GCAGCCAGCG CCACGGCAAA AGCAAGGGCG 251 GCCGCGAGTT GAGCTTGAGC GGGCGTGATT TGGCCGGTTT TTTGGTGGAT 301 TGCTCCGCGC CGCAGCTCAA TGTAAAGGGC ATGACGGTAT TGGATGCAGC 351 CAAAAAGCTG GCCGCGCCGT GGCCGCAGAT TAAAGCGGTG GTGCTTAAGG 401 TCGAAAACAA CCCCGCTTTG GACAAAATCG ACATCGAGCC GGGCGAAACC 451 GTATGGCAGG CATTAACCCA TATTGCCAAC TCGGTCGGGC TGCATCCGTG 501 GCTGGAGCCG GACGGCACGT TGGTGGTGGG CGGTGTGGAT TACAGCAGCC 551 CGCCGGTGGC GACATTGTGT TGGAGCCGCA CCGACAGCCG CCGCAATATC 601 GAGCGCATGG ACATTGAGTG GGATACCGAC AACCGCTTTT CTGAGGTTAC 651 TTTTTTGGCG CAATCGCACG GCCGCAGCGG CGACAGCGCC AAACACGATT 701 TAAAGTGGGT GTACAAAGAC CCGACGATGA CGCTGCACCG CCCTAAAACG 751 GTGGTGGTGT CCGATGCCGA CAATTTGGCC GCATTGCAAA AGCAGGCTAA 801 AAAGCAGCTG GCCGACTGGC GGCTGGAGGG ATTTACACTC ACGATAACCG 851 TGGGCGGCCA TAAAACCCGC GACGGCGTAT TGTGGCAACC TGGCCAGCGT 901 GTGCATGTGA TCGACGACGA GCACGGTATC GATGCGGTGT TTTTTCTGAT 951 GGGGCGGCGG TTTATGCTAT CTCGCATGGA TGGCACGCAA ACCGAGCTGC 1001 GGCTCAAAGA GGACGGTATT TGGACACCCG ACGCTTACCC CAAAAAGGCC 1051 GAGGCGGCGC GCAAGCGCAA AGGCAAACGC AAAGGCGTGA GCCATAAGGG 1101 CAAAAAAGGC GGCAAAAAAC AAGCAGAAAC GGCGGTGTTT GAATGA This corresponds to the amino acid sequence <SEQ ID 2398; ORF 713. a>: a713.pep 1 MQNNSYGYAV SVRVGGKEHR HWERYDIDSD FLIPADSFDF VIGRLGPEAA 51 IPDLSGESCE WIDGQIVMT GIIGSQRHGK SKGGRELSLS GRDLAGFLVD 101 CSAPQLNVKG MTVLDAAKKL AAPWPQIKAV VLKVENNPAL DKIDIEPGET 151 VWQALTHIAN SVGLHPWLEP DGTLVVGGVD YSSPPVATLC WSRTDSRRNI 201 ERMDIEWDTD NRFSEVTFLA QSHGRSGDSA KHDLKWVYKD PTMTLHRPKT 251 WVSDADNLA ALQKQAKKQL ADWRLEGFTL TITVGGHKTR DGVLWQPGQR 301 VHVIDDEHGI DAVFFLMGRR FMLSRMDGTQ TELRLKEDGI WTPDAYPKKA 351 EAARKRKGKR KGVSHKGKKG GKKQAETAVF E* a713/m713 98.4% identity in 381 aa overlap 10 20 30 40 50 60 a713.pep MQNNSYGYAVSVRVGGKEHRHWERYDIDSDFLIPADSFDFVIGRLGPEAAIPDLSGESCE I II II II II I II II I II II II II II II I II II II II II II II II II I II II I II I II II I m713 MQNNSYGYAVSVRVGGKEHRHWERYDIDSDFLIPADSFDFVIGRLGPEAAIPDLSGESCE 10 20 30 40 50 60 70 80 90 100 110 120 a713.pep WIDGQIVMTGIIGSQRHGKSKGGRELSLSGRDLAGFLVDCSAPQLNVKGMTVLDAAKKL II I II II II II II II II II II I I : II I II I II II I I II I II II I I II II I I I II I II II I m713 VVIDGQIVMTGIIGSQRHGKSKGSRELSLSGRDLAGFLVDCSAPQLNVKGMTVLDAAKKL 70 80 90 100 110 120 130 140 150 160 170 180 a713.pep AAPWPQIKAWLKVENNPALDKIDIEPGETVWQALTHIANSVGLHPWLEPDGTLWGGVD I II II II I II II I : II II II II I II II I II II II II I II II II II I II II II II II I: I m713 AAPWPQIKAWLKAENNPALGKIDIEPGETVWQALTHIANSVGLHPWLEPDGTLVVGGAD 130 140 150 160 170 180 190 200 210 220 230 240 a 13.pep YSSPPVATLCWSRTDSRRNIERMDIEWDTDNRFSEVTFLAQSHGRSGDSAKHDLKWVYKD II II II II II I II II II II II I II II I I I I II I I II I II II II II II II I I II I I II I I m713 YSSPPVATLCWSRTDSRCNIERMDIEWDTDNRFSEVTFLAQSHGRSGDSAKHDLKWVYKD 190 200 210 220 230 240 250 260 270 280 290 300 a713. pep PTMTLHRPKTVWSDADNLAALQKQAKKQLADWRLEGFTLTITVGGHKTRDGVLWQPGQR I II II II I II I II I II II I I II I II II II II II I II II II II I I II II I I II I I II II I m713 PTMTLHRPKTVWSDADNLAALQKQAKKQLADWRLEGFTLTITVGGHKTRDGVLWQPGLR 250 260 270 280 290 300 310 320 330 340 350 360 a713.pep VHVIDDEHGIDAVFFLMGRRFMLSRMDGTQTELRLKEDGIWTPDAYPKKAEAARKRKGKR II I II I II II I II I II II II II II II II II I II II II I I II II I II II I II II II II II I m713 VHVIDDEHGIDAVFFLMGRRFMLSRMDGTQTELRLKEDGIWTPDAYPKKAEAARKRKGKR 310 320 330 340 350 360 370 380 a713. pep KGVSHKGKKGGKKQAETAVFEX I I I I I II II II II I II I I II II m713 KGVSHKGKKGGKKQAETAVFEX 370 380 g714.seq not found yet g714.pep not found yet The following partial DNA sequence was identified in N meningitidis <SEQ ID 2399>: m714.seq 1 ATGAGCTATC AAGACATCTT GCGGGGCCTG TTGCCCCCCG TGTCGTATGC 51 CCGCAATGCC CCGCGTGTGC GGGCGCAGGC AGAAATAGAC GGCGCAGCGC 101 TGGATGCGGT GGCGGAATCG GCTCAAAGCG TTGCCGATGC CGTCGACCCG 151 CGCAGCGCCG GCCAAATGCT GGCCGATTGG GAGCGCGTAT TAGGTTTGGA 201 CGGTACGGGC AAAAACCGCC AGCACCGTGT GTTGGCCGTC ATGGCCAAGC 251 TAAACGAAAC AGGCGGCTTG AGTATTCCTT ATTTTGTGCG TTTGGCCGAG 301 GCGGCGGGCT ATCAAATCCA AATCGACGAA CCGCAGCCGT TCCGCGCCGG 351 TGTAAACCGC GCCGGCGACC GTCTTGCGCC GCAGGAAATC ATGTGGGTGT 401 GGCACGTTAA CGTGCGCGGC GGCAACAACC GCATTACCCG ATTCCGCGCC 451 GGTATCTCGG CGGCGGGCGA CAGGCTGACC GATTACAGCG ATGCCGTGAT 501 CGAGAGCCTG TTCAACCGCC TCAAGCCCGC CCACACCGCT ATCCGATTTA 551 CCTACCGCTA A This corresponds to the amino acid sequence <SEQ ID 2400; ORF 714>: m714.pep 1 MSYQDILRGL LPPVSYARΝA PRVRAQAEID GAALDAVAES AQSVADAVDP 51 RSAGQMLADW ERVLGLDGTG KΝRQHRVLAV MAKLΝETGGL SIPYFVRLAE 101 AAGYQIQIDE PQPFRAGVΝR AGDRLAPQEI MWVWHVΝVRG GΝΝRITRFRA 151 GISAAGDRLT DYSDAVIESL FΝRLKPAHTA IRFTYR* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2401>: a714.seq 1 ATGAGCTATC AAGACATCTT GCGGGGTCTG TTGCCCCCCG TGTCGTATGC 51 CCGCAATGCC CCGCGTGTGC GGGCGCAGGC AGAAATAGAC GGCGCAGCGC 101 TGGATGCGGT GGCGGAATCG GCTCAAAGCG TTGCCGATGC CGTCGACCCG 151 AGCAGCGCCG GCCAAATGCT GGCCGATTGG GAGCGCGTAT TAGGTTTGGA 201 CGGTACGGGC AAAAACCGCC AGCGCCGTGT GTTGGCCGTC ATGGCCAAGC 251 TAAACGAAAC AGGCGGCTTG AGTATTCCTT ATTTTGTGCG TTTGGCCGAG 301 GCGGCGGGCT ATCAAATCCA AATCGACGAA CCGCAGCCGT TCCGCGCCGG 351 TGTAAACCGC GCCGGCGACC GTCTTGCGCC GCAGGAAATC ATGTGGGTGT 401 GGCACGTTAA CGTGCGCGGC GGCAACAACC GCATTACCCG ATTCCGCGCC 451 GGTATCTCGG CGGCGGGCGA CAGGCTGACC GATTACAGCG ATGCCGTGAT 501 CGAGAGCCTG TTCAACCGCC TCAAGCCCGC CCACACCGCT ATCCGATTTA 551 CCTACCGATA A This corresponds to the amino acid sequence <SEQ ID 2402; ORF 714.a>: a714.pep 1 MSYQDILRGL LPPVSYARΝA PRVRAQAEID GAALDAVAES AQSVADAVDP 51 SSAGQMLADW ERVLGLDGTG KΝRQRRVLAV MAKLΝETGGL SIPYFVRLAE 101 AAGYQIQIDE PQPFRAGVΝR AGDRLAPQEI MWVWHVΝVRG GΝΝRITRFRA 151 GISAAGDRLT DYSDAVIESL FΝRLKPAHTA IRFTYR* a714/m714 98.9% identity in 186 aa overlap 10 20 30 40 50 60 a714. pep MSYQDILRGLLPPVSYARΝAPRVRAQAEIDGAALDAVAESAQSVADAVDPSSAGQMLADW I II II II I II II II I II I I II II II I I II I I I I I I I II II II II I II I II I II I II I II m714 MSYQDILRGLLPPVSYARΝAPRVRAQAEIDGAALDAVAESAQSVADAVDPRSAGQMLADW 10 20 30 40 50 60 70 80 90 100 110 120 a714. pep ERVLGLDGTGKΝRQRRVLAVMAKLΝETGGLSIPYFVRLAEAAGYQIQIDEPQPFRAGVΝR II M II II II II II : II II I I II I II II I II I I I II II I I II II II II I II I I I I II II I m714 ERVLGLDGTGKΝR Q HRVLAVMAKLΝETGGLSIPYFVRLAEAAGYQIQIDEPQPFRAGVΝR 70 80 90 100 110 120 130 140 150 160 170 180 a714. pep AGDRLAP Q EIMWVWHVΝVRGGΝΝRITRFRAGISAAGDRLTDYSDAVIESLFΝRLKPAHTA I M II I I II I II I II II I II II I I II I I I II I II I II II II I I I II I II II I II II II II m714 AGDRLAPQEIMWVWHVNVRGGNNRITRFRAGISAAGDRLTDYSDAVIESLFNRLKPAHTA 130 140 150 160 170 180 a714.pep IRFTYRX m714 IRFTYRX g715.seq not found yet g715.peρ not found yet The following partial DNA sequence was identified in N meningitidis <SEQ ID 2403>: m715. seq 1 ATGATTGATG TCAAAATAGA CAATATCTTT GTCGTCCTAA ACCAAATCGA 51 GCGGCTTGGC AACGGGATCG AAAACCGCTA CCTGCTGATG CGCCGACTGT 101 CCGAAACCAT GCACACGGCG GTCAAGCTCA ATTTCCGCTA CGCAGGCCGT 151 CCGAAATGGG TTGGGCTAAA ATACCGCGAC GGCAAGCCGC TTTCGGATTC 201 GGGTCGTCTG AAAGACAGTT TTTCCACACT GTCAGACAAC GATACAGCCC 251 TTGTCGGTAC GAATATCGTC TATGCCGCCA TCCACAACTT CGGCGGTATG 301 GCGGGGCGCA ACCGCAAAGT TCGGATTCCG CAACGGGAAT TTTTGACGCT 351 GACGGACGAC GACAAACAGG CTTTGATGGA CGATGTGCAG GATTATTTTT 401 CGGGTCTGAT ACCGTGA This corresponds to the amino acid sequence <SEQ ID 2404; ORF 715>: 715.pep 1 MIDVKIDΝIF WLΝQIERLG ΝGIEΝRYLLM RRLSETMHTA VKLΝFRYAGR 51 PKWVGLKYRD GKPLSDSGRL KDSFSTLSDΝ DTALVGTΝIV YAAIHΝFGGM 101 AGRΝRKVRIP QREFLTLTDD DKQALMDDVQ DYFSGLIP* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2405>: a715.seq 1 ATGATTGATG TCAAAATAGA CAATATCTTT GTCGTCCTAA ACCAAATCGA 51 GCGGCTTGGC AACGGGATCG AAAACCGCTA CCTGCTGATG CGCCGACTGT 101 CCGAAACCAT GCACACGGCG GTCAAGCTCA ATTTCCGCTA CGCAGGCCGT 151 CCGAAATGGT TGGGGCTAAA ATACCGCGAC GGCAAGCCGC TTTCGGATTC 201 GGGTCGTCTG AAAGACAGTT TTTCCACACT GTCAGACAAC GATACAGCCC 251 TTGTCGGTAC GAATATCGTC TATGCCGCCA TCCACAACTT CGGCGGTATG 301 GCGGGGCGCA ACCGCAAAGT TCGGATTCCG CAACGGGAAT TTTTGACGCT 351 GACGGACGAC GACAAACAGG CTTTGATGGA CGATGTGCAG GATTATTTTT 451 CGGGTCTGAT ACCGTGA This corresponds to the amino acid sequence <SEQ ID 2406; ORF 715.a> a715.pep 1 MIDVKIDΝIF WLΝQIERLG ΝGIEΝRYLLM RRLSETMHTA VKLΝFRYAGR 51 PKWLGLKYRD GKPLSDSGRL KDSFSTLSDΝ DTALVGTΝIV YAAIHΝFGGM 101 AGRΝRKVRIP QREFLTLTDD DKQALMDDVQ DYFSGLIP* The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 2407>: g716.seq 1 ATGAACAAAA ATATTGCTGC CGCACTCGCC GGTGCTTTAT CCCTGTCTCT 51 GGCCGCCGGC GCCGTTGCCG CCCACAAACC GGCAAGCAAC GCAACAGGCG 101 TTCAAAAATC CGCCCAAGGC TCTTGCGGCG CATCCAAATC TGCCGAAGGT 151 TCGTGCGGCG CATCCAAATC TGCCGAAGGT TCGTGCGGCG CGGCTGCTTC 201 TAAAGCAGGC GAAGGCAAAT GCGGCGAGGG CAAATGCGGT GCAACTGTAA 251 AAAAAGCCCA CAAACACACC AAAGCATCTA AAGCCAAAGC CAAATCTGCC 301 GAAGGCAAAT GCGGCGAAGG CAAATGCGGT TCTAAATAA This corresponds to the amino acid sequence <SEQ ID 2408; ORF 716.ng>: g716.pep 1 MΝKΝIAAALA GALSLΞLAAG AVAAHKPASΝ ATGVQKSAQG SCGAΞKSAEG 51 ΞCGAΞKSAEG SCGAAASKAG EGKCGEGKCG ATVKKAHKHT KASKAKAKSA 101 EGKCGEGKCG SK* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2409>: 716.seq 1 ATGAACAAAA ACATTGCTGC CGCTCTCGCC GGTGCTTTAT CCCTGTCTTT 51 GGCCGCCGGT GCAGTTGCTG CCAACAAACC GGCAAGCAAC GCAACAGGCG 101 TTCATAAATC CGCCCATGGC TCTTGCGGCG CGTCCAAATC TGCCGAAGGT 151 TCGTGCGGCG CGGCTGGTTC TAAAGCAGGC GAAGGCAAAT GCGGCGAGGG 201 CAAATGCGGT GCGACCGTAA AAAAAACCCA CAAACACACC AAAGCATCTA 251 AAGCCAAGGC CAAATCTGCC GAAGGCAAAT GCGGCGAAGG CAAATGCGGT 301 TCTAAATAA This corresponds to the amino acid sequence <SEQ ID 2410; ORF 716>: 716.pep 1 MΝKΝIAAALA GALSLSLAAG AVAAΝKPASΝ ATGVHKSAHG SCGASKSAEG 51 SCGAAGSKAG EGKCGEGKCG ATVKKTHKHT KASKAKAKSA EGKCGEGKCG 101 SK* m716/g716 86.6% identity in 112 aa overlap 10 20 30 40 50 m716.pep MΝKΝIAAALAGALSLSLAAGAVAAΝKPASΝATGVHKSAHGSCGASKSAEGSCGA I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I : I I I : I I I I I I I I I I I I I I I g716 MΝKΝIAAALAGALΞLSLAAGAVAAHKPASΝATGVQKSAQGSCGASKSAEGSCGASKSAEG 10 20 30 40 50 60 60 70 80 90 100 m716.pep AGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKΞAEGKCGEGKCGSKX Ml I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I g716 SCGAAASKAGEGKCGEGKCGATVKKAHKHTKASKAKAKSAEGKCGEGKCGSKX 70 80 90 100 110 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2411>: a716.seq 1 ATGAACAAAA ACATTGCTGC CGCACTCGCC GGTGCTTTAT CCCTGTCTTT 51 GGCCGCCGGT GCAGTTGCTG CCAACAAACC GGCAAGCAAC GCAACAGGCG 101 TTCATAAATC CGCCCATGGC TCTTGCGGCG CGTCCAAATC TGCCGAAGGT 151 TCGTGCGGCG CGGCTGGTTC TAAAGCAGGC GAAGGCAAAT GCGGCGAGGG 201 CAAATGCGGT GCGACCGTAA AAAAAACCCA CAAACACACC AAAGCATCTA 251 AAGCCAAGGC CAAATCTGCC GAAGGCAAAT GCGGCGAAGG CAAATGCGGT 301 TCTAAATAA The following partial DΝA sequence was identified in N meningitidis <SEQ LD 2412. a>: a716.pep 1 MΝKΝIAAALA GALSLSLAAG AVAAΝKPASΝ ATGVHKSAHG SCGASKSAEG 51 SCGAAGSKAG EGKCGEGKCG ATVKKTHKHT KASKAKAKSA EGKCGEGKCG 101 SK* a716/m716 100.0% identity in 102 aa overlap 10 20 30 40 50 60 a716. pep MΝKΝIAAALAGALSLSLAAGAVAAΝKPASΝATGVHKSAHGSCGASKSAEGSCGAAGSKAG II I II II II I II II I II II I II II I I II I II II I II II II I II II II I I II I II I II I I I m716 MΝKΝIAAALAGALSLSLAAGAVAAΝKPASΝATGVHKSAHGSCGASKSAEGSCGAAGSKAG 10 20 30 40 50 60 70 80 90 100 a716. pep EGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSKX II II I I II I I I II I I II II II I II II II II I II II II I I II II m716 EGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSKX 70 80 90 100 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 2413>: g717.seq 1 ATGGACACAA AAGAAATCCT CGGCTACGCG GCAGGCTCGA TCGGCAGCGC 51 GGTTTTAGCC GTCATCATCC TGCCGCTGCT GTCGTGGTAT TTCcccgCCG 101 ACGACATCGG GCGCATCGTG CTGATGCAGA CGGCGGCGGG ACTGACGGTG 151 TCGGTATTGT GCCTCGGGCT GGATCAGGCA TACGTCCGCG AATACTATGC 201 CGCCGCCGAC AAAGACACTT TGTTCAAAAC CCTGTTCCTG CCGCCGCTGC 251 TGTTTTCCGC CGCGATAGCC GCCCTGCTGC TTTCCCGCCC GTCCCTGCCG 301 TCTGAAATCC TGTTTTCGCT CGACGATGCC GCCGCCGGCA TCGGGCTGGT 351 GCTGTTTGAA CTGAGCTTCC TGCCCATCCG CTTTCTCTTA CTGGTTTTGC 401 GTATGGAAGG GCGCGCCCTT GCCTTTTCGT CCGCGCAACT CGTGCCCAAA 451 CTCGCCATTC TGCTGCTGTT GCCGCTGACG GTCGGGCTGC TGCACTTTCC 501 GGCGAACACC TCCGTCCTGA CCGCCGTTTA CGCGCTGGCA AACCTTGCCG 551 CCGCCGCCTT TTTGCTGTTT CAAAACCGAT GCCGTCTGAA GGCCGTCCGG 601 CGCGCGCCGT TTTCGCCCGC CGTCCTGCAC CGGGGGCTGC GCTACGGCAT 651 ACCGCTCGCA CTGAGCAGCC TTGCCTATTG GGGGCTGGCA TCCGCCGACC 701 GTTTGTTCCT GAAAAAATAT GCGGGCCTGG AACAGCTCGG CGTTTATTCG 751 ATGGGTATTT CGTTCGGCGG GGCGGCATTA TTGCTCCAAA GCATCTTTTC 801 AACGGTCTGG ACACCGTATA TTTTCCGTGC AATCGAAGAA AACGCCACGC 851 CCGCCCGCCT CTCGGCAACG GCAGAATCCG CCGCCGCCCT GCTTGCCTCC 901 GCCCTCTGCC TGACCGGAAT TTTCTCGCCC CTCGCCTCCC TCCTGCTGCC 951 GGAAAACTAC GCCGCCGTCC GGTTTACCGT CGTATCGTGT ATGCTGccgc 1001 cgctGTTTTA CACGCTGACC GAAATCAGCG GCATCGGTTT GAACGTCGTC 1051 CGCAAAACGC GTCCGATCGC GCTTGCCACC TTGGGCGCGC TGGCGGCAAA 1101 CCTGCTGCTG CTGGGGCTTG CCGTACCGTC CGGCGGCACG CGCGGCGCGG 1151 CGGTTGCCTG TGCCGCCTCA TTCTGGTTGT TTTTTGTTTT CAAGACAGAA 1201 AGCTCCTGCC GCCTGTGGCA GCCGCTCAAA CGCCTGCCGC TTTATATGCA 1251 CACATTGTTC TGCCTgGCCT CCTCGGCGGC CTACACCTGC TTCGGCACAC 1301 CGGCAAACTA CCCcctgttt gccggcgtAT GGGCGGCATA TCTGGCAGGC 1351 TGCATCCTGC GCCACCGGAA AAATTTGCAC AAACTGTTTC ATTATTTGAA 1401 AAAACAAGGT TTCCCATTAT GA This corresponds to the amino acid sequence <SEQ ID 2414; ORF 717.ng>: g717.pep 1 MDTKEILGYA AGSIGΞAVLA VIILPLLSWY FPADDIGRIV LMQTAAGLTV 51 SVLCLGLDQA YVREYYAAAD KDTLFKTLFL PPLLFΞAAIA ALLLSRPSLP 101 SEILFSLDDA AAGIGLVLFE LSFLPIRFLL LVLRMEGRAL AFΞSAQLVPK 151 LAILLLLPLT VGLLHFPANT SVLTAVYALA NLAAAAFLLF QNRCRLKAVR 201 RAPFSPAVLH RGLRYGIPLA LSSLAYWGLA SADRLFLKKY AGLEQLGVYS 251 MGISFGGAAL LLQSIFSTVW TPYIFRAIEE NATPARLSAT AESAAALLAS 301 ALCLTGIFΞP LAΞLLLPENY AAVRFTVVΞC MLPPLFYTLT EIΞGIGLNVV 351 RKTRPIALAT LGALAANLLL LGLAVPSGGT RGAAVACAAS FWLFFVFKTE 401 SSCRLWQPLK RLPLYMHTLF CLASSAAYTC FGTPANYPLF AGVWAAYLAG 451 CILRHRKNLH KLFHYLKKQG FPL* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2415>: m717.seq 1 ATGGACACAA AAGAAATCCT CGGCTACGCG GCAGGCTCGA TCGGCAGCGC 51 GGTTTTAGCC GTCATCATCC TGCCGCTGCT GTCGTGGTAT TTCCCCGCCG 101 ACGACATCGG GCGCATCGTG CTGATGCAGA CGGCGGCGGG GCTGACGGTG 151 TCGGTGTTGT GCCTCGGGCT GGATCAGGCA TACGTCCGCG AATACTATGC 201 CACCGCCGAC AAAGACACCT TGTTCAAAAC CCTGTTCCTG CCGCCGCTGC 251 TGTCTGCCGC CGCGATAGCC GCCCTGCTGC TTTCCCGCCC GTCCCTGCCG 301 TCTGAAATCC TGTTTTCACT CGACGATGCC GCCGCCGGCA TCGGGCTGGT 351 GCTGTTTGAA CTGAGCTTCC TGCCCATCCG CTTTCTCTTA CTGGTTTTGC 401 GTATGGAAGG ACGCGCCCTT GCCTTTTCGT CCGCGCAACT CGTGCCCAAG 451 CTCGCCATCC TGCTGCTGCT GCCGCTGACG GTCGGGCTGC TGCACTTTCC 501 AGCGAACACC GCCGTCCTGA CCGCCGTTTA CGCGCTGGCA AACCTTGCCG 551 CCGCCGCCTT TTTGCTGTTT CAAAACCGAT GCCGTCTGAA GGCCGTCCGG 601 CACGCACCGT TTTCGCCCGC CGTCCTGCAC CGGGGGCTGC GCTACGGCAT 651 ACCGATCGCA CTGAGCAGCA TCGCCTATTG GGGGCTGGCA TCCGCCGACC 701 GTTTGTTCCT GAAAAAATAT GCCGGCCTGG AACAGCTCGG CGTTTATTCG 751 ATGGGTATTT CGTTCGGCGG GGCGGCATTA TTGTTCCAAA GCATCTTTTC 801 AACGGTCTGG ACACCGTATA TTTTCCGCGC AATCGAAGAA AACGCCCCGC 851 CCGCCCGCCT CTCGGCAACG GCAGAATCCG CCGCCGCCCT GCTTGCCTCC 901 GCCCTCTGCC TGACCGGCAT TTTCTCGCCC CTTGCCTCCC TCCTGCTGCC 951 GGAAAACTAC GCCGCCGTCC GGTTTATCGT CGTATCGTGT ATGCTGCCGC 1001 CGCTGTTTTG CACGCTGGCG GAAATCAGCG GCATCGGTTT GAACGTCGTC 1051 CGCAAAACGC GCCCGATCGC GCTCGCCACC TTGGGCGCGC TGGCGGCAAA 1101 CCTGCTGCTG CTGGGGCTTG CCGTGCCGTC CGGCGGCGCG CGCGGCGCGG 1151 CGGTTGCCTG TGCCGCCTCA TTCTGGCTGT TTTTTGCCTT CAAGACCGAA 1201 AGCTCCTGCC GCCTGTGGCA GCCGCTCAAA CGCCTGCCGC TTTATCTGCA 1251 CACATTGTTC TGCCTGACCT CCTCGGCGGC CTACACCTGC TTCGGCACGC 1301 CGGCAAACTA TCCCCTGTTT GCCGGCGTAT GGGCGGCATA TCTGGCAGGC 1351 TGCATCCTGC GCCACCGGAA AGATTTGCAC AAACTGTTTC ATTATTTGAA 1401 AAAACAAGGT TTCCCATTAT GA This corresponds to the amino acid sequence <SEQ ID 2416; ORF 717>: m.717 pep 1 MDTKEILGYA AGSIGΞAVLA VIILPLLSWY FPADDIGRIV LMQTAAGLTV 51 SVLCLGLDQA YVREYYATAD KDTLFKTLFL PPLLSAAAIA ALLLSRPSLP 101 SEILFSLDDA AAGIGLVLFE LSFLPIRFLL LVLRMEGRAL AFSSAQLVPK 151 LAILLLLPLT VGLLHFPANT AVLTAVYALA NLAAAAFLLF QNRCRLKAVR 201 HAPFSPAVLH RGLRYGIPIA LSΞIAYWGLA SADRLFLKKY AGLEQLGVYS 251 MGISFGGAAL LFQSIFSTVW TPYIFRAIEE NAPPARLSAT AESAAALLAS 301 ALCLTGIFSP LAΞLLLPENY AAVRFIVVSC MLPPLFCTLA EISGIGLNVV 351 RKTRPIALAT LGALAANLLL LGLAVPΞGGA RGAAVACAAS FWLFFAFKTE 401 SSCRLWQPLK RLPLYLHTLF CLTSΞAAYTC FGTPANYPLF AGVWAAYLAG 451 CI RHRKDLH KLFHYLKKQG FPL* m717/g717 96.4% identity in 473 aa overlap 10 20 30 40 50 60 m717.pep MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQTAAGLTVΞVLCLGLDQA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g717 MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQTAAGLTVSVLCLGLDQA 10 20 30 40 50 60 70 80 90 100 110 120 m717.pep YVREYYATADKDTLFKTLFLPPLLSAAAIAALLLSRPΞLPSEILFΞLDDAAAGIGLVLFE I I I I I I I : I I I I I I I I I I I I I I I I M I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g717 YVREYYAAADKDTLFKTLFLPPLLFSAAIAALLLSRPSLPSEILFSLDDAAAGIGLVLFE 70 80 90 100 110 120 130 140 150 160 170 180 m717.pep LΞFLPIRFLLLVLRMEGRALAFSSAQLVPKLAILLLLPLTVGLLHFPANTAVLTAVYALA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I g717 LΞFLPIRFLLLVLRMEGRALAFSSAQLVPKLAILLLLPLTVGLLHFPANTΞVLTAVYALA 130 140 150 160 170 180 190 200 210 220 230 240 m717.pep NLAAAAFLLFQNRCRLKAVRHAPFSPAVLHRGLRYGIPIALΞΞIAYWGLASADRLFLKKY I I I I I I I I I I I I I I I I I I I Ml I I I I I I I I I II I I I I Ml I I I : I I I I I I I I I I I I I I I I g717 NLAAAAFLLFQNRCRLKAVRRAPFSPAVLHRGLRYGIPLALSΞLAYWGLAΞADRLFLKKY 190 200 210 220 230 240 250 260 270 280 290 300 m717. pep AGLEQLGVYΞMGIΞFGGAALLFQSIFSTVWTPYIFRAIEENAPPARLSATAESAAALLAS I I I I I I I I I I I I I I I I I I I I Ml I I I I I I I I I I I I I I I I I I I l ll g717 AGLEQLGVYSMGISFGGAALLLQΞIFΞTVWTPYIFRAIEENATPARLΞATAEΞAAALLAS 250 260 270 280 290 300 310 320 330 340 350 360 m717.pep ALCLTGIFSPLAΞLLLPENYAAVRFIVVΞCMLPPLFCTLAEISGIGLNVVRKTRPIALAT I I I I I I I I I I I II I II I I II I I I I I l I l I I I I I I I I I I I I I I I I I I I g717 ALCLTGIFSPLASLLLPENYAAVRFTVVΞCMLPPLFYTLTEISGIGLNVVRKTRPIALAT 310 320 330 340 350 360 370 380 390 400 410 420 m717. pep LGALAANLLLLGLAVPSGGARGAAVACAASFWLFFAFKTESSCRLWQPLKRLPLYLHTLF I I I II I I II I I I I I I I I I I : I I I I I I I I I I I I 1 I Ml I I I I I I I I I II I I I I I I I : I I I I g717 LGALAANLLLLGLAVPSGGTRGAAVACAAΞFWLFFVFKTESSCRLWQPLKRLPLYMHTLF 370 380 390 400 410 420 430 440 450 460 470 m717.pep CLTSSAAYTCFGTPANYPLFAGVWAAYLAGCILRHRKDLHKLFHYLKKQGFPLX I MM I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I g717 CLASΞAAYTCFGTPANYPLFAGVWAAYLAGCILRHRKNLHKLFHYLKKQGFPLX 430 440 450 460 470 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2417>: a717. seq 1 ATGGACACAA AAGAAATCCT CGGCTACGCG GCAGGCTCGA TCGGCAGCGC 51 GGTTTTAGCC GTCATCATCC TGCCGCTGCT GTCGTGGTAT TTCCCTGCCG 101 ACGACATCGG ACGCATCGTG CTGATGCAGA CGGCGGCGGG GCTGACGGTG 151 TCGGTGTTGT GCCTCGGGCT GGATCAGGCA TACGTCCGCG AATACTATGC 201 CGCCGCCGAC AAAGACACTT TGTTCAAAAC CCTGTTCCTG CCGCCGCTGC 251 TGTCTGCCGC CGCGATAGCC GCCCTGCTGC TTTCCCGCCC ATCCCTGCCG 301 TCTGAAATCC TGTTTTCGCT CGACGATGCC GCCGCCGGCA TCGGGCTGGT 351 GCTGTTTGAA CTGAGCTTCC TGCCCATCCG CTTTCTCTTA CTGGTTTTGC 401 GTATGGAAGG ACGCGCCCTT GCCTTTTCGT CCGCGCAACT CGTGTCCAAG 451 CTCGCCATCC TGCTGCTGCT GCCGCTGACG GTCGGGCTGC TGCACTTTCC 501 GGCGAACACC GCCGTCCTGA CCGCCGTTTA CGCGCTGGCA AACCTTGCCG 551 CCGCCGCCTT TTTGCTGTTT CAAAACCGAT GCCGTCTGAA GGCCGTCCGG 601 CGCGCACCGT TTTCATCCGC CGTCCTGCAT CGCGGCCTGC GCTACGGCAT 651 ACCGATCGCA CTAAGCAGCA TCGCCTATTG GGGGCTGGCA TCCGCCGACC 701 GTTTGTTCCT GAAAAAATAT GCCGGCCTAG AACAGCTCGG CGTTTATTCG 751 ATGGGTATTT CGTTCGGCGG AGCGGCATTA TTGTTCCAAA GCATCTTTTC 801 AACGGTCTGG ACACCGTATA TTTTCCGCGC AATCGAAGCA AACGCCCCGC 851 CCGCCCGCCT CTCGGCAACG GCAGAATCCG CCGCCGCCCT GCTTGCCTCC 901 GCCCTCTGCC TGACCGGCAT TTTCTCGCCC CTCGCCTCCC TCCTGCTGCC 951 GGAAAACTAC GCCGCCGTCC GGTTTATCGT CGTATCGTGT ATGCTGCCTC 1001 CGCTGTTTTG CACGCTGGTA GAAATCAGCG GCATCGGTTT GAACGTCGTC 1051 CGAAAAACAC GCCCGATCGC GCTCGCCACC TTGGGCGCGC TGGCGGCAAA 1101 CCTGCTGCTG CTGGGGCTTG CCGTACCGTC CGGCGGCGCG CGCGGCGCGG 1151 CGGTTGCCTG TGCCGCCTCA TTTTGGCTGT TTTTTGTTTT CAAGACCGAA 1201 AGCTCCTGCC GCCTGTGGCA GCCGCTCAAA CGCCTGCCGC TTTATATGCA 1251 CACATTGTTC TGCCTGGCCT CCTCGGCGGC CTACACCTGC TTCGGCACTC 1301 CGGCAAACTA CCCCCTGTTT GCCGGCGTAT GGGCGGTATA TCTGGCAGGC 1351 TGCATCCTGC GCCACCGGAA AGATTTGCAC AAACTGTTTC ATTATTTGAA 1401 AAAACAAGGT TTCCCATTAT GA This corresponds to the amino acid sequence <SEQ ID 2418; ORF 717.a>: a717.pep 1 MDTKEILGYA AGSIGSAVLA VIILPLLSWY FPADDIGRIV LMQTAAGLTV 51 SVLCLGLDQA YVREYYAAAD KDTLFKTLFL PPLLSAAAIA ALLLSRPSLP 101 SEILFSLDDA AAGIGLVLFE LSFLPIRFLL LVLRMEGRAL AFSSAQLVSK 151 LAILLLLPLT VGLLHFPANT AVLTAVYALA NLAAAAFLLF QNRCRLKAVR 201 RAPFSSAVLH RGLRYGIPIA LSSIAYWGLA SADRLFLKKY AGLEQLGVYS 251 MGISFGGAAL LFQSIFSTVW TPYIFRAIEA NAPPARLSAT AESAAALLAS 301 ALCLTGIFSP LASLLLPENY AAVRFIWSC MLPPLFCTLV EISGIGLNW 351 RKTRPIALAT LGALAANLLL LGLAVPSGGA RGAAVACAAS FWLFFVFKTE 401 SSCRLWQPLK RLPLYMHTLF CLASSAAYTC FGTPANYPLF AGVWAVYLAG 451 CILRHRKDLH KLFHYLKKQG FPL* a717/m717 97.9% identity in 473 aa overlap 10 20 30 40 50 60 a717.pep MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQTAAGLTVSVLCLGLDQA II II I II I II II I I I II I II I I II I I II II II I II I I I I II I I II II II I II II I II II I m717 MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQTAAGLTVSVLCLGLDQA 10 20 30 40 50 60 70 80 90 100 110 120 a717.pep YVREYYAAADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSEILFSLDDAAAGIGLVLFE II II II I: II I II II I I I II II II II II I I I II II II I I II II I I II I II II II II II II m717 YVREYYATADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSEILFSLDDAAAGIGLVLFE 70 80 90 100 110 120 130 140 150 160 170 180 a717.pep LSFLPIRFLLLVLRMEGRALAFSSAQLVSKLAILLLLPLTVGLLHFPANTAVLTAVYALA I I II II II II II II II II II I II II II I II I II I II I II II I I II I II II I II II I I II ϊtl717 LSFLPIRFLLLVLRMEGRALAFSSAQLVPKLAILLLLPLTVGLLHFPANTAVLTAVYALA 130 140 150 160 170 180 190 200 210 220 230 240 a717.pep NLAAAAFLLFQNRCRLKAVRRAPFSSAVLHRGLRYGIPIALSSIAYWGLASADRLFLKKY I I II I II I II I II II I II II : II II II I II I II I II I II II II II I I I II II I II II I I m717 NLAAAAFLLFQNRCRLKAVRHAPFSPAVLHRGLRYGIPIALSSIAYWGLASADRLFLKKY 190 200 210 220 230 240 250 260 270 280 290 300 a717.pep AGLE Q LGVYSMGISFGGAALLFQSIFSTVWTPYIFRAIEANAPPARLSATAESAAALLAS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m717 AGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFRAIEENAPPARLSATAESAAALLAS 250 260 270 280 2 90 300 310 320 330 340 350 360 a717 . pep ALCLTGIFSPLASLLLPENYAAVRFIWSCMLPPLFCTLVEISGIGLNWRKTRPIALAT MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM m717 ALCLTGIFSPLASLLLPENYAAVRFIWSCMLPPLFCTLAEISGIGLNWRKTRPIALAT 310 320 330 340 350 360 370 380 390 400 410 420 a717.pep LGALAANLLLLGLAVPSGGARGAAVACAASFWLFFVFKTESSCRLWQPLKRLPLYMHTLF I II I II I II I II II I II II I II II II II I I I II II : I II II II I I I II I II II I M I I II m717 LGALAANLLLLGLAVPSGGARGAAVACAASFWLFFAFKTESSCRLWQPLKRLPLYLHTLF 370 380 390 400 410 420 430 440 450 460 470 a717. pep CLASSAAYTCFGTPANYPLFAGVWAVYLAGCILRHRKDLHKLFHYLKKQGFPLX I I : I II I I II II II II II I I II I II : II I I II II I II II II I II II II II II II m717 CLTSSAAYTCFGTPANYPLFAGVWAAYLAGCILRHRKDLHKLFHYLKKQGFPLX 430 440 450 460 470 g 18.seq not found yet g718.pep not found yet The following partial DNA sequence was identified in N meningitidis <SEQ ID 2419>: m718.seq 1 TCAGACGGCC TTTACGTACC CCGAAACTTT ATCCACCGCC CGCAAAGCTG 51 GTTCAAATGG GACAAAGACA ACGGGCTGCT GCTGCGTACC CGCGAAAATC 101 CGGAAGGCGA AGCGTTGTGG CCGCTGGGCT GGGTCGTTCA TACCCAAAAA 151 TCGCGCAGCG TCCAGCAGGC GCGCAACGGG CTTTTCCGCA CGCTTTCCTG 201 GCTGTATATG TTCAAACACT ACGCCGTCCA CGATTTTGCC GAGTTTTTGG 251 AGCTGTACGG CATGCCCATC CGTATCGGCA AATACGGCGC GGGCGCAACC 301 AAAGAGGAAA AAAACACCCT GCTTCGAGCG GTGGCGGAAA TCGGTCACAA 351 CGCGGCAGGC ATCATGCCAG AAGGTATGGA AATAGAGCTC CACAACGCGG 401 CAAACGGTAC GACGGCAACC AGCAATCCGT TTTTGCAGAT GGCCGACTGG 451 TGCGAAAAAT CGGCGGCGCG GCTGATTTTG GGGCAAACGC TGACCAGCGG 501 TGCGGACGGA AAATCCAGCA CCAACGCGCT GGGCAATATC CACAACGAGG 551 TACGCCGCGA TTTGCTGGTG TCGGACGCAA AACAGGTGGC GCAAACCATC 601 ACAAGCCAAA TCATCGGACC GTTCCTGCAA ATCAACTATC CCCATGCCGA 651 CCCAAACCGC GTGCCGAAAT TTGAATTTGA CACGCGCGAG CCGAAAGACA 701 TCGCGGTCTT TGCCGACGCT ATCCCGAAAC TGGTGGATGT CGGCGTACAA 751 ATCCCCGAAA GCTGGGTGCG CGACAAACTG GTCATTCCAG ATGTGCAGGA 801 GGGTGAGGCT GTGTTGGTGC GGCAGGTACC GGACAATCCG GTAAACAGAA 851 CTGCATTGGC GGCTTTATCC GCCCACACCG TACCATCTAA GGCTACGGGC 901 AGGCATCAGG AAATATTGGA CGGCGCGTTG GATGACGCGC TGGTTGAGCC 951 CGATTTCAAT TCTCAGCTCA ACCCGATGGT GCGTCAGGCG GTTGCCGCAC 1001 TTAATGCTTG CAACAGCTAC GAGGAGGCAG ATGCCGCACT GAATGCGCTT 1051 TATCCGAATT TGGACAACGC GAAACTGCGT ACCTATATGC AGCAGGCCTT 1101 GTTTATCAGC GATATTTTGG GACAAGACCA TGCCCGCGCC TGA This corresponds to the amino acid sequence <SEQ ID 2420; ORF 718>: m718.pep 1 SDGLYVPRΝF IHRPQSWFKW DKDΝGLLLRT REΝPEGEALW PLGWWHTQK 51 SRSVQQARΝG LFRTLSWLYM FKHYAVHDFA EFLELYGMPI RIGKYGAGAT 101 KEEKΝTLLRA VAEIGHΝAAG IMPEGMEIEL HΝAAΝGTTAT SΝPFLQMADW 151 CEKSAARLIL GQTLTSGADG KSSTΝALGΝI HΝEVRRDLLV SDAKQVAQTI 201 TSQIIGPFLQ IΝYPHADPΝR VPKFEFDTRE PKDIAVFADA IPKLVDVGVQ 251 IPESWVRDKL VIPDVQEGEA VLVRQVPDΝP VΝRTALAALS AHTVPSKATG 301 RHQEILDGAL DDALVEPDFΝ SQLΝPMVRQA VAALΝACΝSY EEADAALΝAL 351 YPΝLDΝAKLR TYMQQALFIS DILGQDHARA * The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2421 > • a718. seq 1 ATGGAGCCGA TAATGGCAAA AAAGAACAAT AAAACTAAAA TCCAAAAGCC 51 CGAAGCTGCA TTGCAGACGG ACGTGGCTCA AATTACAGCG ACCGGTCGAG 101 TTATCGCCGA GCATCCATCC AATTTTATTA CGCCGCAAAA GATGCGCGCC 151 CTCTTCGAGG ACGCAGAAAG CGGTGACATC CGCGCCCAAC ACGAGCTTTT 201 CGCGGACATT GAGGAGCGCG ACAGCGACAT CGCGGCAAAT ATGGGGACGC 251 GCAAACGCGC GCTGCTGACG CTCAACTGGC GCGTCGCCCC GCCGCGAAAT 301 GCGACGCCCG AAGAAGAAAA GCTGTCCGAC CAAGCCTACG AAATGATGGA 351 CAGCCTGCCT ACCCTCGAAG ACCTGATTAT GGATTTGATG GACGCGGTAG 401 GGCACGGATT TTCTGCGTTG GAGGTCGAGT GGGTATTTTC AGACGGCCTT 451 TACCTACCCC GAAACTTTAT CCACCGCCCG CAAAGCTGGT TCAAATGGGA 501 CAAAGACAAC GGGCTGCTGC TGCGTACCCG CGAAAATCCG GAAGGCGAAG 551 CGTTGTGGCC GCTGGGCTGG GTCGTTCATA CCCAAAAATC GCGCAGCGTC 601 CAGCAGGCGC GCAACGGGCT TTTCCGCACG CTTTCCTGGC TGTATATGTT 651 CAAACACTAC GCCGTCCACG ATTTTGCCGA GTTTTTGGAG CTGTACGGCA 701 TGCCCATCCG TATCGGCAAA TACGGCGCGG GCGCAACCAA AGAGGAAAAA 751 AACACCCTGC TTCGAGCGGT GGCGGAAATC GGTCACAACG CGGCAGGCAT 801 CATGCCAGAA GGTATGGAAA TCGAGCTGCA CAACGCGGCA AACGGCATGA 851 CTTCCGCCGG CAATCCGTTT TTGCAGATGG CCGACTGGTG CGAAAAATCG 901 GCGGCGCGGC TGATTTTGGG GCAAACGCTA ACCAGCGGTG CGGACGGAAA 951 ATCCAGCACC AACGCGCTGG GCAATATCCA CAACGAGATA CGCCGCGATT 1001 TGCTGGTGTC GGACGCAAAA CAGGTGGCGC AAACCATCAC AAGCCAAATC 1051 ATCGGACCGT TCCTGCAAAT CAACTATCCC CATGCCGACC CAAACCGCGT 1101 GCCGAAATTT GAATTTGACA CGCGCGAGCC GAAAGACATC GCGGTCTTTG 1151 CCGACGCTAT CCCGAAACTG GTGGATGTCG GCGTACAAAT CCCCGAAAGC 1201 TGGGTGCGCG ACAAACTGGT CATTCCAGAT GTGCAGGAGG GTGAGGCTGT 1251 GTTGGTGCGG CAGGTACCGG ACAATCCGGT AAACAGAACT GCATTGGCGG 1301 CTTTATCCGC CCACACCGTA CCATCTAAGG CTACGGGCAG GCATCAGGAA 1351 ATATTGGACG GCGCGTTGGA TGACGCGCTG GTTGAGCCCG ATTTCAATTC 1401 TCAGCTCAAC CCGATGGTGC GTCAGGCGGT TGCCGCACTT AATGCTTGCA 1451 ACAGCTACGA GGAGGCAGAT GCCGCACTGA ATGCGCTTTA TCCGAATTTG 1501 GACAACGCGA AACTGCGTAC CTATATGCAG CAGGCCTTGT TTATCAGCGA 1551 TATTTTGGGA CAAGACCATG CCCGCGCCTG A This corresponds to the amino acid sequence <SEQ ID 2422; ORF 718.a>: a718.pep 1 MEPIMAKKNN KTKIQKPEAA LQTDVAQITA TGRVIAEHPS NFITPQKMRA 51 LFEDAESGDI RAQHELFADI EERDSDIAAN MGTRKRALLT LNWRVAPPRN 101 ATPEEEKLSD QAYEMMDSLP TLEDLIMDLM DAVGHGFSAL EVEWVFSDGL 151 YLPRNFIHRP QSWFKWDKDN GLLLRTRENP EGEALWPLGW WHTQKSRSV 201 QQARNGLFRT LSWLYMFKHY AVHDFAEFLE LYGMPIRIGK YGAGATKEEK 251 NTLLRAVAEI GHNAAGIMPE GMEIELHNAA NGMTSAGNPF LQMADWCEKS 301 AARLILGQTL TSGADGKSST NALGNIHNEI RRDLLVSDAK QVAQTITSQI 351 IGPFLQINYP HADPNRVPKF EFDTREPKDI AVFADAIPKL VDVGVQIPES 401 WVRDKLVIPD VQEGEAVLVR QVPDNPVNRT ALAALSAHTV PΞKATGRHQE 451 ILDGALDDAL VEPDFNSQLN PMVRQAVAAL NACNSYEEAD AALNALYPNL 501 DNAKLRTYMQ QALFISDILG QDHARA* a718/m718 98.4% identity in 380 aa overlap 120 130 140 150 160 170 a718. pep DSLPTLEDLIMDLMDAVGHGFSALEVEWVFSDGLYLPRNFIHRPQSWFKWDKDNGLLLRT II II I : I I I II II I II II I I II II I II II I m718 SDGLYVPRNFIHRPQSWFKWDKDNGLLLRT 10 20 30 180 190 200 210 220 230 a718 . pep RENPEGEALWPLGWVVHTQKSRSVQQARNGLFRTLSWLYMFKHYAVHDFAEFLELYGMPI I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m718 RENPEGEALWPLGWWHTQKSRSVQQARNGLFRTLSWLYMFKHYAVHDFAEFLELYGMPI 40 50 60 70 80 90 240 250 260 270 280 290 a718.pep RIGKYGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAANGMTSAGNPFLQMADW II II I II II I I II I II II II I II II II II II II II I I I II II II II I :: : II I I I II II m718 RIGKYGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAANGTTATSNPFLQMADW 100 110 120 130 140 150 300 310 320 330 340 350 a718.pep CEKSAARLILGQTLTSGADGKSSTNALGNIHNEIRRDLLVSDAKQVAQTITSQIIGPFLQ II II I II I II I II II I I I II I I II II II II I II : II I I II I II II II I I II I I II I II II m718 CEKSAARLILGQTLTSGADGKSSTNALGNIHNEVRRDLLVSDAKQVAQTITSQIIGPFLQ 160 170 180 190 200 210 360 370 380 390 400 410 a718. pep INYPHADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQIPESWVRDKLVIPDVQEGEA MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM m718 INYPHADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQIPESWVRDKLVIPDVQEGEA 220 230 240 250 260 270 420 430 440 450 460 470 a718.pep VLVRQVPDNPVNRTALAALSAHTVPSKATGRHQEILDGALDDALVEPDFNSQLNPMVRQA I II II II II I II II I II II II II II II I II II II II I I II I II I I I I I II II I I II I II I m718 VLVRQVPDNPVNRTALAALSAHTVPSKATGRHQEILDGALDDALVEPDFNSQLNPMVRQA 280 290 300 310 320 330 480 490 500 510 520 a718.pep VAALNACNSYEEADAALNALYPNLDNAKLRTYMQQALFISDILGQDHARAX II I II II II I II II I II II II II II II I II I II II II I II I II II I II II I m718 VAALNACNSYEEADAALNALYPNLDNAKLRTYMQQALFISDILGQDHARAX 340 350 360 370 380 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2423>: m718-l.seq 1 ATGGAGCCGA TAATGGCAAA AAAGAACAAT AAAACTAAAA TCCAAAAGCC 51 CGAAGCTGCA TTGCAGACGG ACGTGGCTCA AATTACGGCG ACCGGTCGGG 101 TTATCGCCGA GCATCCGTCC AATTTTATTA CGCCGCAAAA GATGCGGGCC 151 CTCTTCGAGG ACGCAGAAAG CGGCGACATC CGCGCCCAAC ACGAGCTTTT 201 CGCGGACATT GAGGAGCGCG ACAGCGACAT CGCGGCAAAT ATGGGGACGC 251 GCAAACGCGC GCTGCTGACG CTCAACTGGC GCGTCGCCCC GCCGCGAAAT 301 GCGACGCCCG AAGAAGAAAA GCTGTCCGAC CAAGCCTACG AAATGATGGA 351 CAGCCTGCCT ACCCTCGAAG ACCTGATTAT GGATTTGATG GACGCGGTAG 401 GGCACGGATT TTCTGCGTTG GAGGTCGAGT GGGTATTTTC AGACGGCCTT 451 TACCTACCCC GAAACTTTAT CCACCGCCCG CAAAGCTGGT TCAAATGGGA 501 CAAAGACAAC GGGCTGCTGC TGCGTACCCG CGAAAATCCG GAAGGCGAAG 551 CGTTGTGGCC GCTGGGCTGG GTCGTTCATA CCCAAAAATC GCGCAGCGTC 601 CAGCAGGCGC GCAACGGGCT TTTCCGCACG CTTTCCTGGC TGTATATGTT 651 CAAACACTAC GCCGTCCACG ATTTTGCCGA GTTTTTGGAG CTGTACGGCA 701 TGCCCATCCG TATCGGCAAA TACGGCGCGG GCGCAACCAA AGAGGAAAAA 751 AACACCCTGC TTCGAGCGGT GGCGGAAATC GGTCACAACG CGGCAGGCAT 801 CATGCCAGAA GGTATGGAAA TAGAGCTCCA CAACGCGGCA AACGGTACGA 851 CGGCAACCAG CAATCCGTTT TTGCAGATGG CCGACTGGTG CGAAAAATCG 901 GCGGCGCGGC TGATTTTGGG GCAAACGCTG ACCAGCGGTG CGGACGGAAA 951 ATCCAGCACC AACGCGCTGG GCAATATCCA CAACGAGGTA CGCCGCGATT 1001 TGCTGGTGTC GGACGCAAAA CAGGTGGCGC AAACCATCAC AAGCCAAATC 1051 ATCGGACCGT TCCTGCAAAT CAACTATCCC CATGCCGACC CAAACCGCGT 1101 GCCGAAATTT GAATTTGACA CGCGCGAGCC GAAAGACATC GCGGTCTTTG 1151 CCGACGCTAT CCCGAAACTG GTGGATGTCG GCGTACAAAT CCCCGAAAGC 1201 TGGGTGCGCG ACAAACTGGT CATTCCAGAT GTGCAGGAGG GTGAGGCTGT 1251 GTTGGTGCGG CAGGTACCGG ACAATCCGGT AAACAGAACT GCATTGGCGG 1301 CTTTATCCGC CCACACCGTA CCATCTAAGG CTACGGGCAG GCATCAGGAA 1351 ATATTGGACG GCGCGTTGGA TGACGCGCTG GTTGAGCCCG ATTTCAATTC 1401 TCAGCTCAAC CCGATGGTGC GTCAGGCGGT TGCCGCACTT AATGCTTGCA 1451 ACAGCTACGA GGAGGCAGAT GCCGCACTGA ATGCGCTTTA TCCGAATTTG 1501 GACAACGCGA AACTGCGTAC CTATATGCAG CAGGCCTTGT TTATCAGCGA 1551 TATTTTGGGA CAAGACCATG CCCGCGCCTG A This corresponds to the amino acid sequence <SEQ ID 2424; ORF 718-1>: m718-l.pep. 1 MEPIMAKKΝΝ KTKIQKPEAA LQTDVAQITA TGRVIAEHPS NFITPQKMRA 51 LFEDAESGDI RAQHELFADI EERDSDIAAN MGTRKRALLT LNWRVAPPRN 101 ATPEEEKLΞD QAYEMMDSLP TLEDLIMDLM DAVGHGFSAL EVEWVFSDGL 151 YLPRNFIHRP QSWFKWDKDN GLLLRTRENP EGEALWPLGW WHTQKSRSV 201 QQARNGLFRT LSWLYMFKHY AVHDFAEFLE LYGMPIRIGK YGAGATKEEK 251 NTLLRAVAEI GHNAAGIMPE GMEIELHNAA NGTTATΞNPF LQMADWCEKS 301 AARLILGQTL TSGADGKSST NALGNIHNEV RRDLLVSDAK QVAQTITSQI 351 IGPFLQINYP HADPNRVPKF EFDTREPKDI AVFADAIPKL VDVGVQIPES 401 WVRDKLVIPD VQEGEAVLVR QVPDNPVNRT ALAALSAHTV PSKATGRHQE 451 ILDGALDDAL VEPDFNSQLN PMVRQAVAAL NACNSYEEAD AALNALYPNL 501 DNAKLRTYMQ QALFISDILG QDHARA* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2425>: a718.seq 1 ATGGAGCCGA TAATGGCAAA AAAGAACAAT AAAACTAAAA TCCAAAAGCC 51 CGAAGCTGCA TTGCAGACGG ACGTGGCTCA AATTACAGCG ACCGGTCGAG 101 TTATCGCCGA GCATCCATCC AATTTTATTA CGCCGCAAAA GATGCGCGCC 151 CTCTTCGAGG ACGCAGAAAG CGGTGACATC CGCGCCCAAC ACGAGCTTTT 201 CGCGGACATT GAGGAGCGCG ACAGCGACAT CGCGGCAAAT ATGGGGACGC 251 GCAAACGCGC GCTGCTGACG CTCAACTGGC GCGTCGCCCC GCCGCGAAAT 301 GCGACGCCCG AAGAAGAAAA GCTGTCCGAC CAAGCCTACG AAATGATGGA 351 CAGCCTGCCT ACCCTCGAAG ACCTGATTAT GGATTTGATG GACGCGGTAG 401 GGCACGGATT TTCTGCGTTG GAGGTCGAGT GGGTATTTTC AGACGGCCTT 451 TACCTACCCC GAAACTTTAT CCACCGCCCG CAAAGCTGGT TCAAATGGGA 501 CAAAGACAAC GGGCTGCTGC TGCGTACCCG CGAAAATCCG GAAGGCGAAG 551 CGTTGTGGCC GCTGGGCTGG GTCGTTCATA CCCAAAAATC GCGCAGCGTC 601 CAGCAGGCGC GCAACGGGCT TTTCCGCACG CTTTCCTGGC TGTATATGTT 651 CAAACACTAC GCCGTCCACG ATTTTGCCGA GTTTTTGGAG CTGTACGGCA 701 TGCCCATCCG TATCGGCAAA TACGGCGCGG GCGCAACCAA AGAGGAAAAA 751 AACACCCTGC TTCGAGCGGT GGCGGAAATC GGTCACAACG CGGCAGGCAT 801 CATGCCAGAA GGTATGGAAA TCGAGCTGCA CAACGCGGCA AACGGCATGA 851 CTTCCGCCGG CAATCCGTTT TTGCAGATGG CCGACTGGTG CGAAAAATCG 901 GCGGCGCGGC TGATTTTGGG GCAAACGCTA ACCAGCGGTG CGGACGGAAA 951 ATCCAGCACC AACGCGCTGG GCAATATCCA CAACGAGATA CGCCGCGATT 1001 TGCTGGTGTC GGACGCAAAA CAGGTGGCGC AAACCATCAC AAGCCAAATC 1051 ATCGGACCGT TCCTGCAAAT CAACTATCCC CATGCCGACC CAAACCGCGT 1101 GCCGAAATTT GAATTTGACA CGCGCGAGCC GAAAGACATC GCGGTCTTTG 1151 CCGACGCTAT CCCGAAACTG GTGGATGTCG GCGTACAAAT CCCCGAAAGC 1201 TGGGTGCGCG ACAAACTGGT CATTCCAGAT GTGCAGGAGG GTGAGGCTGT 1251 GTTGGTGCGG CAGGTACCGG ACAATCCGGT AAACAGAACT GCATTGGCGG 1301 CTTTATCCGC CCACACCGTA CCATCTAAGG CTACGGGCAG GCATCAGGAA 1351 ATATTGGACG GCGCGTTGGA TGACGCGCTG GTTGAGCCCG ATTTCAATTC 1401 TCAGCTCAAC CCGATGGTGC GTCAGGCGGT TGCCGCACTT AATGCTTGCA 1451 ACAGCTACGA GGAGGCAGAT GCCGCACTGA ATGCGCTTTA TCCGAATTTG 1501 GACAACGCGA AACTGCGTAC CTATATGCAG CAGGCCTTGT TTATCAGCGA 1551 TATTTTGGGA CAAGACCATG CCCGCGCCTG A This corresponds to the amino acid sequence <SEQ ID 2426; ORF 718-l.a>: a718.pep 1 MEPIMAKKΝΝ KTKIQKPEAA LQTDVAQITA TGRVIAEHPS NFITPQKMRA 51 LFEDAESGDI RAQHELFADI EERDSDIAAN MGTRKRALLT LNWRVAPPRN 101 ATPEEEKLSD QAYEMMDSLP TLEDLIMDLM DAVGHGFSAL EVEWVFΞDGL 151 YLPRNFIHRP QSWFKWDKDN GLLLRTRENP EGEALWPLGW WHTQKSRSV 201 QQARNGLFRT LSWLYMFKHY AVHDFAEFLE LYGMPIRIGK YGAGATKEEK 251 NTLLRAVAEI GHNAAGIMPE GMEIELHNAA NGMTSAGNPF LQMADWCEKS 301 AARLILGQTL TSGADGKSST NALGNIHNEI RRDLLVSDAK QVAQTITSQI 351 IGPFLQINYP HADPNRVPKF EFDTREPKDI AVFADAIPKL VDVGVQIPES 401 WVRDKLVIPD VQEGEAVLVR QVPDNPVNRT ALAALSAHTV PSKATGRHQE 451 ILDGALDDAL VEPDFNSQLN PMVRQAVAAL NACNΞYEEAD AALNALYPNL 501 DNAKLRTYMQ QALFISDILG QDHARA* a718/m718-l 99.0% identity in 526 aa overlap 10 20 30 40 50 60 a718.pep MEPIMAKKNNKTKIQKPEAALQTDVAQITATGRVIAEHPΞNFITPQKMRALFEDAEΞGDI I I I I I I I I I I I I I II I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I Itl718-1 MEPIMAKKNNKTKIQKPEAALQTDVAQITATGRVIAEHPΞNFITPQKMRALFEDAESGDI 10 20 30 40 50 60 70 80 90 100 110 120 a718.pep RAQHELFADIEERDSDIAANMGTRKRALLTLNWRVAPPRNATPEEEKLSDQAYEMMDSLP I I I I I I I II I I I I II I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II m 18-l RAQHELFADIEERDSDIAANMGTRKRALLTLNWRVAPPRNATPEEEKLSDQAYEMMDSLP 70 80 90 100 110 120 130 140 150 160 170 180 a718.pep TLEDLIMDLMDAVGHGFSALEVEWVFSDGLYLPRNFIHRPQΞWFKWDKDNGLLLRTRENP I I I I I I I II I I I I I I I II I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m718-l TLEDLIMDLMDAVGHGFΞALEVEWVFSDGLYLPRNFIHRPQΞWFKWDKDNGLLLRTRENP 130 140 150 160 170 180 190 200 210 220 230 240 a718.pep EGEALWPLGWWHTQKΞRSVQQARNGLFRTLSWLYMFKHYAVHDFAEFLELYGMPIRIGK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m718 -l EGEALWPLGWWHTQKSRSVQQARNGLFRTLSWLYMFKHYAVHDFAEFLELYGMPIRIGK 190 200 210 220 230 240 250 260 270 280 290 300 a718 . pep YGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAANGMTSAGNPFLQMADWCEKS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : : : I I I I I I I I I I I I I m718-l YGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAANGTTATSNPFLQMADWCEKS 250 260 270 280 290 300 310 320 330 340 350 360 a718.pep AARLILGQTLTSGADGKSSTNALGNIHNEIRRDLLVSDAKQVAQTITSQIIGPFLQINYP I I I I I I I I I I I I I I I I I I I I I I I I I I I I IM I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m718-l AARLILGQTLTΞGADGKSSTNALGNIHNEVRRDLLVSDAKQVAQTITSQIIGPFLQINYP 310 320 330 340 350 360 370 380 390 400 410 420 a718. pep HADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQIPESWVRDKLVIPDVQEGEAVLVR I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m718-1 HADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQIPESWVRDKLVIPDVQEGEAVLVR 370 380 390 400 410 420 430 440 450 460 470 480 a718.pep QVPDNPVNRTALAALSAHTVPSKATGRHQEILDGALDDALVEPDFNΞQLNPMVRQAVAAL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m718-1 QVPDNPVNRTALAALΞAHTVPΞKATGRHQEILDGALDDALVEPDFNSQLNPMVRQAVAAL 430 440 450 460 470 480 490 500 510 520 a718.pep NACNSYEEADAALNALYPNLDNAKLRTYMQQALFIΞDILGQDHARAX I I I I I I II II I II II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m718-1 NACNSYEEADAALNA YPNLDNAKLRTYMQQALFISDILGQDHARAX 490 500 510 520 g719.seq not found yet g719.pep not found yet The following partial DNA sequence was identified in N meningitidis <SEQ ID 2427>: m719.seq 1 ATGGCAAACG GGAACATGAA ACTGTCGTTG GTGTTAACCG CCCGAGATGA 51 CGGAGCGAGA CGGCTACTGG CTGATACTCA ACGACAATTA GATCGTACCG 101 CGAAATCGCG GGCGCAACTT GAACGGCAAA GCCATACTTA TGCGTTGACC 151 GGCATCCGCT CAGAAAAACA GATTCAACGC GAAATCATGC TGACACAGGC 201 TGCGTTTAAC CGTTTGGCGC GCAGCGGCAA GGCATCACAA AATGATTTGG 251 CACGGGCGGC GGTCGCTACG CGTAACCGAA TTCGCGAGCT GAACGCGGAA 301 CTGAAACAGG GCACGGGATT TGCGGACAAG ATGGGAAAAA TCGGAAGATT 351 CGGTGCAGCT GCGGTGGCTG GTGGCGCGGC AGCGTATACG GTGCTTAAGC 401 CTGCTATGGA CAACAGAAAG CAGCTTGATG AGAACATCAA CCGCGTGTCC 451 AGACAGGCAT TTATTGAGGA TAACAGTAAA TCGGCAGCGT GGATTGCAAC 501 TGAAGGTGCG CAACAGATCA AGGATTTGGC ACTTGAACTT GTCGAGAAAA 551 ATGGCGGGAC CCACGATAAG GCTTTGGATT TAATCAGCGG CATGATGACC 601 ACCGGTCTGA ATTTTGCCCA AACCAAGAAT GAAGCGCAGG CGGCATATGC 651 TTTTGCACTT GCCTCAGAAG GCAGTGGCGA GGATACGGCA AAACTGATTA 701 AAACCCTGAA AGATGGCGGC ATGAGCGGTA AAGACCTGCA ACTCGGGCTT 751 GAGCACGTCT TGCAATCGGG TTTAGACGGC ACTTTCGAGG TGCGGGATAT 801 GGTTCGGGAG CTGCCGAGCC TGCTCTCTGC CGCGCAACAG GCAGGGATGA 851 ATGGTGTCGG CGGTTTGGAC TACCTGCTCT CACTCTTACA ATCTGCGGCG 901 AATAAATCGG GCAGTCCTGC CGAAGCGGCG ACTAATGTGC AAAATCTTTT 951 GAGTAAAACT CTGTCGCCTG ACACGATAGG TCGTCTGAAG AAGATGGCAA 1001 ATCCGAATGA CCCGAAGAAA GGTGTCGATT GGATAGGCTC GGTTGTGCAA 1051 GGCAAGCAAA ACGGCGAAAA CGCAGTGCAG GTGTTGTCCC GTCTTGCCGA 1101 TGCCATGCTA GTAAAGGATA AGCAATACCA AGATTATAAG AAACGCGCGG 1151 CTGCAGGCGA TAAGACGGCG GCGGAGCAGG CAAATATGCT TAAGGGCGCG 1201 CTTTTGGCGC AACTGCTGCC TGATTTGCAG GCAAAACAAG GTTTGCTGGC 1251 TGCAACGGAT ATGACGCAAA TCCGTGAATA TATGGCTTCG TTGGCTGGCG 1301 TAACGTTGGA TAACGGAAAA ATTGCTAAGA ACAACGAGGC GCGAATGTTG 1351 TCGGCAGCGG CGCAACAAGA GCAACAGGAA TCGCTGGCAA TGTTGCGGGA 1401 AAGTCTGACG GGAACATTGG TGGATATGGA AACCTCGTTT AAAAAGCTGG 1451 CAGCGGAATA CCCTAATGCC ACTCTAGCCC TGCAAGCATT GACGACGGCG 1501 GCAACAGCGG CGTCTGCCGC AATGTTATTA ACCGCCGGTG GCGGTAAAGG 1551 TGCAGGCTTT CTGAAAGATG TAGGTAGTAA AGCGTTGGGA TGGGGTAAGG 1601 CTTCCGCAGG CGGCGTGGCA GCAGGTGCCA CAGCGGCAGG CGGTAAGTTG 1651 CTGTCATGGG GAAAATCTGC CGGTAGCGGG CTCATGAATA ATCCAGCGTT 1701 AGTTAAACGG GCGGGTTTGT TAGGTATGTT GCTGTATTCC GAGTCTTTGG 1751 GTGACGGCAC ATTGCCAAAG GGTTTGCGTG GTACCAAGAC AACTCCTGAA 1801 ATGATTAATC GTCTGAAAAA CAACGGTATC CGATTTGAAC CTGCGCCGAA 1851 GCGGGAACAG GCGCGGGGTG GTGTCCCTCA GTATTTGGCT GCTCCGTCAG 1901 CGCAGCCTAC CGATAAGATG TTGTCTCCGT TGTTTTCAAC TCAGACGGCG 1951 GCGTATCAGG CAGCCATTCA GCAGCAGACG GCGGCGTATC AGGCAGCATT 2001 GGCGCAGGAT ACGGCTGCAG TTACAACAGG TTTGGCACAA GTGCAAAGTG 2051 CGATGGCGTC GGCAAGTCAG ACCATCAATA CCAATGTGAG CCTGAATATC 2101 GACGGACGTG TTATCGCGAA TGAGGTATCG CGGTATCAAG TGGCCATGTT 2151 CGGCCGTGGA GCGGGTCAAT AA This corresponds to the amino acid sequence <SEQ ID 2428; ORF 719>: m719.pep 1 MANGNMKLSL VLTARDDGAR RLLADTQRQL DRTAKSRAQL ERQSHTYALT 51 GIRSEKQIQR EIMLTQAAFN RLARSGKASQ NDLARAAVAT RNRIRELNAE 101 LKQGTGFADK MGKIGRFGAA AVAGGAAAYT VLKPAMDNRK QLDENINRVS 151 RQAFIEDNSK SAAWIATEGA QQIKDLALEL VEKNGGTHDK ALDLISGMMT 201 TGLNFAQTKN EAQAAYAFAL ASEGSGEDTA KLIKTLKDGG MSGKDLQLGL 251 EHVLQSGLDG TFEVRDMVRE LPSLLSAAQQ AGMNGVGGLD YLLSLLQSAA 301 NKSGSPAEAA TNVQNLLSKT LSPDTIGRLK KMANPNDPKK GVDWIGSWQ 351 GKQNGENAVQ VLSRLADAML VKDKQYQDYK KRAAAGDKTA AEQANMLKGA 401 LLAQLLPDLQ AKQGLLAATD MTQIREYMAS LAGVTLDNGK IAKNNEARML 451 SAAAQQEQQE SLAMLRESLT GTLVDMETSF KKLAAEYPNA TLALQALTTA 501 ATAASAAMLL TAGGGKGAGF LKDVGSKALG WGKASAGGVA AGATAAGGKL 551 LSWGKSAGSG LMNNPALVKR AGLLGMLLYS ESLGDGTLPK GLRGTKTTPE 601 MINRLKNNGI RFEPAPKREQ ARGGVPQYLA APSAQPTDKM LSPLFSTQTA 651 AYQAAIQQQT AAYQAALAQD TAAVTTGLAQ VQSAMASASQ TINTNVSLNI 701 DGRVIANEVS RYQVAMFGRG AGQ* a719.seq not found yet a719.pep not found yet g720. seq not found yet g720.pep not found yet The following partial DNA sequence was identified in N meningitidis <SEQ ID 2429>: m720. seq 1 ATGAGCGGAT GGCATACCTT ATTGCAGGAC GCATCTTACA AGGGCGTCGG 51 CTTTGATATT GAGGTGGTGG ACGAGAGCAA CGGCAAGGCA TTGGCCGAGC 101 ATGCGCGGCC GTTTGTGCAG GGTATCGACC TTGAAGACAT GGGCATGACC 151 GGGCGGCAGG TGCAGATTAA TGCGGTGTTT TGGGGCAAGG GCTATGCAGG 201 CCGTCTGAAA AAGCTGCTGG ATGCGCTGGA GCAGCCGGGC GGCGGCGTGC 251 TGGTGCACCC TGTTTGGGGG CGGATGCACA ACATGATTGC GGCATCATGG 301 AGTTACCGAC ATGAGGCCGA TTATGTGGAT TATGCGGGCA TCGATATTAC 351 TTTCCGCGAG GCGGCCGAAG CGCAGGAAAT CTTTGTTTTT GAAAACGCCT 401 TTTTGGTCGA GCTTGAGGCG TTGATTGCTA ATATCGACAC CTACCGCGAG 451 GCGGCTATCG GCTTTGTTGA TGCGGTGTTG GCGGTGGATG CGGGCGTATC 501 AGCTTTATGG GGCAGCGCGC TGGGCATTTG GAGTGCGGCA TCGGGTACGT 551 TTGGCGCGGT GCGCCGTTTG TTTGATTTGG ACAAAATTGC CTTTCCCGAT 601 CGGGGCGGAT ACAGTGCAGC GGCGTTTAAA AACGGCTCGG CCAAGCTGTT 651 TGCGGATATA TCGGTCATGG TAGATACTGG CATACGCCGT GAGGCGGGTT 701 TGGCCGATAA TGCCATGCAC CATGCCGGTT GGTCGCCGCG ACAGCGGTTT 751 GACGGGGCTG CGGCTGTTGC CGACCGCGCC GCCGCTATCC CTGATAATTT 801 GCTGACCGGC CGCTTTTCAG ACGGCCTGCA AAACCGCCTG AACCGGTTAA 851 CCGCCAAACA GGTGCAGCCG GTAGCGCAGG CGGTGCGCCT GTTATCCACG 901 TCATCGCTGT TGTCGGTGGC AACGGCATTA ATCGAGGCGC ATGGCGAAGA 951 GATGACCGCG CCCGATTTGA TTGAGGTTAA CCGCGCCATG CGCCGCCGTA 1001 TGCAGGCCGA GATTGCCGCC TTGCGGGCGG TGCAGACGGC TGCTGCCGAG 1051 TCTGGTGGGC TGACGGCCAA CGCCGTGTAT ACCGAGGCTT ACCAAACGGC 1101 AGAATCCCTG CGCGCGGCGG CAGGCCGTCT GAATGCGTTG GTTGCGGCGG 1151 TCATCAACCA AAAGCCGCCG CTGATTGTGC GCCAAGCCCC AATCGACGGT 1201 ACGATACACC AAATCGCCCA CGAGTTTTAC GGCGATATAG CCCGCGCAGC 1251 AGAGCTGGTG CGGCTCAATC CCCATATCCA CCACCCCGCG TTTATCAAGC 1301 GCGGCACTTT GGTCAACAGC TATGCAAAAT AA This corresponds to the amino acid sequence <SEQ ID 2430; ORF 720>: m720.pep 1 MSGWHTLLQD ASYKGVGFDI EWDESNGKA LAEHARPFVQ GIDLEDMGMT 51 GRQVQINAVF WGKGYAGRLK KLLDALEQPG GGVLVHPVWG RMHNMIAASW 101 SYRHEADYVD YAGIDITFRE AAEAQEIFVF ENAFLVELEA LIANIDTYRE 151 AAIGFVDAVL AVDAGVSALW GSALGIWSAA SGTFGAVRRL FDLDKIAFPD 201 RGGYSAAAFK NGSAKLFADI SVMVDTGIRR EAGLADNAMH HAGWSPRQRF 251 DGAAAVADRA AAIPDNLLTG RFSDGLQNRL NRLTAKQVQP VAQAVRLLST 301 SSLLSVATAL IEAHGEEMTA PDLIEVNRAM RRRMQAEIAA LRAVQTAAAE 351 SGGLTANAVY TEAYQTAESL RAAAGRLNAL VAAVINQKPP LIVRQAPIDG 401 TIHQIAHEFY GDIARAAELV RLNPHIHHPA FIKRGTLVNS YAK* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2431>: a720.seq (partial) 1 GGCCTGCAAA ACCGCCTGAA CCGGTTAACC GCCAAACAGG TGCAGCCGGT 51 AGCGCAGGCG GTGCGCCTGT TATCCACGTC ATCGCTGTTG TCGGTGGCAA 101 CGGCATTAAT CGAGGCGCAT GGCGAAGAGA TGACCGCGCC CGATTTGATT 151 GAGGTTAACC GCGCCATGCG CCGCCGTATG CAGGCCGAGA TTGCCGCCTT 201 ACGGGCGGTG CAGACGGCTG CTGCCGAGTC TGGTGGGCTG ACGGCCAACG 251 CCGTGTATAC CGAGGCTTAC CAAACGGCAG AATCCCTGCG CGCGGCGGCA 301 GGCCGTCTGA ATGCGTTGGT TGCGGCGGTC ATCAACCAAA AGCCGCCGCT 351 GATTGTGCGC CAAGCCCCAA TCGACGGTAC GATACACCAA ATCGCCCACG 401 AGTTTTACGG CGATATAGCC CGCGCAGCAG AGCTGGTGCG GCTCAATCCC 451 CATATCCACC ACCCCGCGTT TATCAAGCGC GGCACTTTGG TCAACAGCTA 501 TGCAAAATAA This corresponds to the amino acid sequence <SEQ ID 2432; ORF 720.a>: a720.pep (partial) 1 GLQΝRLΝRLT AKQVQPVAQA VRLLSTSSLL SVATALIEAH GEEMTAPDLI 51 EVΝRAMRRRM QAEIAALRAV QTAAAESGGL TAΝAVYTEAY QTAESLRAAA 101 GRLΝALVAAV IΝQKPPLIVR QAPIDGTIHQ IAHEFYGDIA RAAELVRLΝP 151 HIHHPAFIKR GTLVΝSYAK* m720 / a720 100.0% identity in 169 aa overlap 250 260 270 280 290 300 m720. pep SPRQRFDGAAAVADRAAAIPDΝLLTGRFSDGLQΝRLΝRLTAKQVQPVAQAVRLLSTSSLL II I II II I I II II II II I I I II II I I II I I a720 GLQΝRLΝRLTAKQVQPVAQAVRLLSTSSLL 10 20 30 310 320 330 340 350 360 m720. pep SVATALIEAHGEEMTAPDLIEVΝRAMRRRMQAEIAALRAVQTAAAESGGLTAΝAVYTEAY II II II II I II I II II I II I I I I II II I II I II I I I II I I I II II II II II I I I I II II I a720 SVATALIEAHGEEMTAPDLIEVΝRAMRRRMQAEIAALRAVQTAAAESGGLTAΝAVYTEAY 40 50 60 70 80 90 370 380 390 400 410 420 m720.pep QTAESLRAAAGRLΝALVAAVIΝQKPPLIVRQAPIDGTIHQIAHEFYGDIARAAELVRLΝP I II II I II I I II I I I II II I II II I II I II I II II I II II II I II I II II II I II II I II a720 QTAESLRAAAGRLΝALVAAVIΝQKPPLIVRQAPIDGTIHQIAHEFYGDIARAAELVRLΝP 100 110 120 130 140 150 430 440 HIHHPAFIKRGTLVΝSYAKX a720 HIHHPAFIKRGTLVNSYAKX 160 170 g721.seq not found g721.pep not found The following partial DNA sequence was identified in N meningitidis <SEQ ID 2433>: m721. seq 1 ATGTCCAAAA ATGCACAAAA AACCCTACTT GCCGTGTGCA GTTTCGAGGT 51 GCAGCCAAAA GACGGGCGAA TCCAACTGCT GCCATATGGC GAATTTCGCG 101 CAGTAGACGG TCGTCCGACT GATGTCCCTG CGTGGTATCT GACCGAAGAA 151 AACGGTCATG ATGTCGCGTT GTTGGCCAAC AGCTCGCGCA ATCAGTTGGT 201 TGTCGATTAT GAACACCAGA CGCTCTACAA AGAGAAAAAC GGACAACCTG 251 CACCTGCCGC CGGTTGGATG CGTTGGCTGG AGTTCACGCC TAAAGGCATG 301 TTTGCCGAAG TGGAGTGGAC GGACAAGGCG GCTGCGGCAA TTGCCGCAAA 351 AGAGTATCGC TACATCTCTG CTGTGTTTTC CTATGACACA AAGGGATATG 401 TAAGCAAAAT TTTTCACGCC GCGCTGACAA ATTTCCCCGC GTTGGACGGT 451 ATGGACGAGG TGCTGGCGGC AGCGTCGGCG CAAATTTTAA AACCGGAAAC 501 GGAGCAAAAC CCTATGAAAG AGTTGTTACA GCAACTGTTC GACCTGCCTG 551 ATGCGGGCGA AGAAGAACTG AAGGCGGCAT TGTCCGCGCT CGTGGAAGCC 601 AAGCCGAAAG ACGTGGCATT GTCTGCCGAC GTGTTCGCGC AGCTGGCGGA 651 AAAAGACAGC CGCATCGCGG CATTGACGGC GCAAACCGCC AAGCCTGATT 701 TGACTAAATA CGCGCCTATC TCAGTGGTTC AAGAGCTGCA AAGCAAAGTC 751 GCCGCGCTGA CTGCCAAGCA GGAAGCAGAC AAAGGCAACG AATTGATTAC 801 CGCCGCGCTG ACTTCAGGCA AATTGCTGCC TGCTCAGAAG GAGTGGGCAA 851 AAGGCGTATT GAAACAGCCG GGCGGCTTGG CATTTTTGAC CGGCTTTATT 901 GAAAACGCCC AGCCGGTCGC TGCACTGGCA GGCTCGCAAA CGGGCGGCAA 951 AGCACCCGAC GAACGCGTCG CCGCACTGAC TGCGGAAGAG GCAGCCGCAG 1001 CAAAAATGCT GGGCATGTCC GGCGAAGAAT TTGTAAAAAT CAAAGAAAGC 1051 GAAGGTAAGT AA This corresponds to the amino acid sequence <SEQ LD 2434; OI 1>: 721.pep 1 MSKΝAQKTLL AVCSFEVQPK DGRIQLLPYG EFRAVDGRPT DVPAWYLTEE 51 ΝGHDVALLAΝ SSRΝQLVVDY EHQTLYKEKΝ GQPAPAAGWM RWLEFTPKGM 101 FAEVEWTDKA AAAIAAKEYR YIΞAVFSYDT KGYVSKIFHA ALTΝFPALDG 151 MDEVLAAAΞA QILKPETEQΝ PMKELLQQLF DLPDAGEEEL KAALΞALVEA 201 KPKDVALSAD VFAQLAEKDS RIAALTAQTA KPDLTKYAPI SVVQELQSKV 251 AALTAKQEAD KGΝELITAAL TSGKLLPAQK EWAKGVLKQP GGLAFLTGFI 301 EΝAQPVAALA GSQTGGKAPD ERVAALTAEE AAAAKMLGMS GEEFVKIKES 351 EGK* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2435>: a721. seq 1 ATGTCCAAAA ATGCACAAAA AACCCTACTT GCCGTGTGCA GTTTCGAGGT 51 GCAGCCAAAA GACGGGCGAA TCCAACTGCT GCCATATGGC GAATTTCGCG 101 CAGTAGACGG TCGTCCGACT GATGTCCCTG CGTGGTATCT GACCGAAGAA 151 AACGGTCATG ATGTCGCGTT GTTGGCCAAC AGCTCGCGCA ATCAGTTGGT 201 TGTCGATTAT GAACACTAGA CGCTCTACAA AGAGAAAAAC GGACAACCTG 251 CACCTGCCGC CGGTTGGATG CGTTGGCTGG AGTTCACGCC TAAAGGCATG 301 TTTGCCGAAG TGGAGTGGAC GGACAAGGCG GCTGCGGCAA TTGCCGCAAA 351 AGAGTATCGC TACATCTCTG CTGTGTTTTC CTATGACACA AAGGGATATG 401 TAAGCAAAAT TTTTCACGCC GCGCTGACAA ATTTCCCCGC GTTGGACGGT 451 ATGGACGAGG TGCTGGCGGC AGCGTCGGCG CAAATTTTAA AACCGGAAAC 501 GGAGCAAAAC CCTATGAAAG AGTTGTTACA GCAACTGTTC GGTCTGCCTG 551 ATGCGGGCGA AGAAGAACTG AAGGCGGCAT TGTCCGCGCT CGTGGAAGCC 601 AAGCCGAAAG ACGTGGCATT GTCTGCCGAC GTGTTCGCGC AGCTGGCGGA 651 AAAAGACAGC CGCATCGCGG CATTGACGGC GCAAACCGCC AAGCCTGATT 701 TGACTAAATA CGCGCCTATC TCAGTGGTTC AAGAGCTGCA AAGCAAAGTC 751 GCCGCGCTGA CTGCCAAGCA GGAAGCAGAC AAAGGCAACG AATTGATTAC 801 CGCCGCGCTG ACTTCAGGCA AATTGCTGCC TGCTCAGAAG GAGTGGGCAG 851 AAGGCGTATT GAAACAGCCG GGCGGCTTGG CATTTTTGAC CGGCTTTATT 901 GAAAACGCCC AGCCGGTCGC TGCACTGGCA GGCTCGCAAA CGGGCGGTAA 951 AGCACCCGAC GAACGCGTCG CCGCACTGAC TGCGGAAGAG GCAGCCGCAG 1001 CAAAAATGCT GGGCATGTCC GGCGAAGAAT TTGTAAAAAT CAAAGAAAGC 1051 GAAGGTAAGT AA This corresponds to the amino acid sequence <SEQ ID 2436; ORF 721.a>: a721.pep 1 MSKNAQKTLL AVCSFEVQPK DGRIQLLPYG EFRAVDGRPT DVPAWYLTEE 51 NGHDVALLAN SSRNQL DY EH*TLYKEKN GQPAPAAGWM RWLEFTPKGM 101 FAEVEWTDKA AAAIAAKEYR YISAVFSYDT KGYVSKIFHA ALTNFPALDG 151 MDEVLAAASA QILKPETEQN PMKELLQQLF GLPDAGEEEL KAALSALVEA 201 KPKDVALSAD VFAQLAEKDS RIAALTAQTA KPDLTKYAPI SVVQELQSKV 251 AALTAKQEAD KGNELITAAL TSGKLLPAQK EWAEGVLKQP GGLAFLTGFI 301 ENAQPVAALA GSQTGGKAPD ERVAALTAEE AAAAKMLGMS GEEFVKIKES 351 EGK* a721/m721 99.2% identity in 353 aa overlap 10 20 30 40 50 60 a721. pep MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPAWYLTEENGHDVALLAN II II I II II I II II I II II II II II I II I I II II II II I II II I II II II II I I II I II I m 721 MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPAWYLTEENGHDVALLAN 10 20 30 40 50 60 70 80 90 100 110 120 a721. pep SSRNQLVVDYEHXTLYKEKNGQPAPAAGWMRWLEFTPKGMFAEVEWTDKAAAAIAAKEYR I II II II II I II II II II II II II I I I I I II II I I I II I II II I II II I II II II II II m721 SSRNQLWDYEHQTLYKEKNGQPAPAAGWMRWLEFTPKGMFAEVEWTDKAAAAIAAKEYR 70 80 90 100 110 120 130 140 150 160 170 180 a721. pep YIΞAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASAQILKPETEQNPMKELLQQLF I I II I II II I II M II II II II II II I I I II I II I II II II II II II II II I II II I II I m 721 YISAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASAQILKPETEQNPMKELLQQLF 130 140 150 160 170 180 190 200 210 220 230 240 a721. pep GLPDAGEEELKAALSALVEAKPKDVALSADVFAQLAEKDSRIAALTAQTAKPDLTKYAPI II I I I II I II II II II II I I II I I II II I II II II II II II II II II II II II II II II m721 DLPDAGEEELKAALSALVEAKPKDVALSADVFAQLAEKDSRIAALTAQTAKPDLTKYAPI 190 200 210 220 230 240 250 260 270 280 290 300 a721. pep SWQELQSKVAALTAKQEADKGNELITAALTSGKLLPAQKEWAEGVLKQPGGLAFLTGFI I I I II II I I I II II II II II II II I I I II II I II II I I II II M II II I I II II II I I II m721 SWQELQSKVAALTAKQEADKGNELITAALTSGKLLPAQKEWAKGVLKQPGGLAFLTGFI 250 260 270 280 290 300 310 320 330 340 350 a721. pep ENAQPVAALAGSQTGGKAPDERVAALTAEEAAAAKMLGMSGEEFVKIKESEGKX I II II I II I I II I II II II II II II II II I II II II I I II II I II I I II I I I I I m721 ENAQPVAALAGSQTGGKAPDERVAALTAEEAAAAKMLGMSGEEFVKIKESEGKX 310 320 330 340 350 g722. seq not found yet g722.pep not found yet The following partial DNA sequence was identified in N meningitidis <SEQ ID 2437>: m722. seq 1 GTGTTTGAAA CGCCGACATT TGAGCAAATC CGCGAGCGTA TCCTGCGCGA 51 TACCAAAAGC CTGTGGCCGG ATGCCGATAT CAGCCCCGAC AGCGACCATT 101 ATGTGCACGC CAGCCGTTTG GCCAGCTGCG CCGAAGGGCA ATATGCGCAT 151 CAAAGCTGGA TTGTGCGGCA GATTTTCCCT GATACCGCCG ACCGCGAGTA 201 TTTGGAGCGG CATGCCTCCA TGCGCGGCTT GAGCCGCCGC AATCCTACCA 251 CGGCCAGCGG CACGCTGACC GTAAGCGGTA TTGCGCAATC CATGCTTTCA 301 GACGACCTGC AAGTGCGTAT CGGCCAGCGT TTTTACCGCA CTACCGCCCG 351 CGCCGTTATC GGCAGCGGCG GCACGGCGGA AATACCGGCA ATCGCCGACG 401 AGCCGGGCGC GGCCGCCAAT GTGGGCGACG GCGAGGCGCA ACTGATGGCC 451 GCCCCCGCCG GTGTGGCCAC CGAATGCCGC CTTACCGTAC AAGGCGGCAC 501 CGACCGAGAA AGCGATGCCT CACTGCTGGC GCGTCTGTTG GAAATCATCC 551 GCCGACCGCC CGCAGGCGGC AACCGTTACG ACTATAAAAA CTGGGCGTTG 601 AGTGTTGACG GCGTAACCAG CGCATATGTT TATCCGCTGC GCCGCGGCTT 651 GGGTACGGTG GATATTGCCA TTACCTCCGC CGACGGTGTG TCGTCGGAAG 701 AAACTGTGCG CCGCGTACAG GCTTATATCG ACGAGATGCG CCCGGTAACG 751 GCAAAAAATG CGCTGGTACT CAAGCCAACC GTAACGGCGG TGCCTGTTAC 801 CGTGCAAGTC AAGCTCGACG GTATCGACTT GGACGAGGCC AAGCGCCGCA 851 TACGGACGGC CCTAAAAGAA TATTTCGACA CCCTGATCCC CGGCGACGGC 901 CTGACTGTGT CGCAAATCGA GGCTGCTATC AGCAATGTGG ATGGTGTGAT 951 CGACCGCCGT CTGACTGCGC CGACGGCCAA CCGTGCCGCC GATACGGTTA 1001 ACCGCATCGA GTGGTTTAAA GCGGGCGCGA TTAATGTAAC GGAGATGCCG 1051 TCATGA This corresponds to the amino acid sequence <SEQ ID 2438; ORF 722>: m722.pep 1 VFETPTFEQI RERILRDTKS LWPDADISPD SDHYVHASRL ASCAEGQYAH 51 QSWIVRQIFP DTADREYLER HASMRGLSRR NPTTASGTLT VSGIAQSMLS 101 DDLQVRIGQR FYRTTARAVI GSGGTAEIPA IADEPGAAAN VGDGEAQLMA 151 APAGVATECR LTVQGGTDRE SDASLLARLL EIIRRPPAGG NRYDYKNWAL 201 SVDGVTSAYV YPLRRGLGTV DIAITSADGV SSEETVRRVQ AYIDEMRPVT 251 AKNALVLKPT VTAVPVTVQV KLDGIDLDEA KRRIRTALKE YFDTLIPGDG 301 LTVSQIEAAI SNVDGVIDRR LTAPTANRAA DTVNRIEWFK AGAINVTEMP 351 S* The following partial DNA sequence was identified inN meningitidis <SEQ ID 2439>: a722.seq 1 GTGTTTGAAA CGCCGACATT TGAGCAAATC CGCGAGCGTA TCCTGCGCGA 51 TACCAAAAGC CTGTGGCCGG ATGCCGATAT CAGCCCCGAC AGCGACCATT 101 ATGTGCACGC CAGCCGTTTG GCCAGCTGCG CCGAAGGGCA ATATGCGCAT 151 CAAAGCTGGA TTGTGCGGCA GATTTTCCCT GATACCGCCG ACCGCGAGTA 201 TTTGGAGCGG CATGCCTCCA TGCGCGGCTT GCGCCGCCGC AATCCTACCA 251 CGGCCAGCGG CACGCTGACC GTAAGCGGTA TTGCGCAATC CATGCTTTCA 301 GACGGCCTGC AAGTGCGTAT CGGCCAGCGT TTTTACCGCA CTACCGCCCG 351 CGCCGTTATC GGCAGCGGCG GCACGGCGGA AATACCGGCA ATCGCCGACG 401 AGCCGGGCGC GGCCGCCAAT GTGCGCGACG GCGAGGCGCA ACTGATGGCC 451 GCCCCCGCCG GTGTGTCCAC CGAATGCCGC CTTACCGTAC AAGGCGGCAC 501 CGACCGAGAA AGCGATGCCT CACTGCTGGC GCGTCTGTTG GAAATCATCC 551 GCCGACCGCC CGCAGGCGGC AACCGTTACG ACTATAAAAA CTGGGCGTTG 601 AGTGTTGACG GCGTAACCAG CGCATATGTT TATCCGCTGC GCCGCGGCTT 651 GGGTACGGTG GATATTGCCA TTACCTCCGC CGACGGTGTG CCATCGGAAG 701 AAACTGTGCG CCGCGTACAG GCTTATATCG ACGAGATGCG CCCGGTAACG 751 GCAAAAAATG CGCTGGTACT CAAGCCAACC GTAACGGCGG TGCCTGTTAC 801 CGTGCAAGTC AAGCTCGACG GCATCGACTT GGACGAGGCC AAGCGCCGCA 851 TACGGACGGC CCTAAAAGAA TATTTCGACA CCCTGATCCC CGGCGACGGC 901 CTGACTGTGT CGCAAATCGA GGCGGCTATC AGCAATGTGG ATGGTGTGAT 951 CGACCTCCGT CTGACTGCGC CGACGGCCAA CCGTGCCGCC GATACGGTTA 1001 ACCGCATCGA GTGGTTTAAA GCGGGCGCGA TTAATGTAAC GGAGATGCCG 1051 TCATGA This corresponds to the amino acid sequence <SEQ ID 2440; ORF 722.a>: a722.pep 1 VFETPTFEQI RERILRDTKS LWPDADISPD SDHYVHASRL ASCAEGQYAH 51 QSWIVRQIFP DTADREYLER HASMRGLRRR NPTTASGTLT VSGIAQSMLS 101 DGLQVRIGQR FYRTTARAVI GSGGTAEIPA IADEPGAAAN VRDGEAQLMA 151 APAGVSTECR LTVQGGTDRE SDASLLARLL EIIRRPPAGG NRYDYKNWAL 201 SVDGVTSAYV YPLRRGLGTV DIAITSADGV PSEETVRRVQ AYIDEMRPVT 251 AKNALVLKPT VTAVPVTVQV KLDGIDLDEA KRRIRTALKE YFDTLIPGDG 301 LTVSQIEAAI SNVDGVIDLR LTAPTANRAA DTVNRIEWFK AGAINVTEMP 351 S* g723.seq not found yet g723.peρ not found yet The following partial DNA sequence was identified in N meningitidis <SEQ ID 2441>: m723.seq 1 ATGCGACCCA AGCCCCGTTT CAGACGGTCT GTTATCGCTT GCTCAATATC 51 AGTGATCACG CCCGAACACC TTATTTTTAC CGTTTACAAA CACAATACCG 101 TCTTCGCCCG CGGCCACTTC TTCGCCGCTA TCATCCACGC CCAGCTGCAC 151 TTCGCCTTTG GCCATAGCAC GCAGCAGGTC GAGCACGTCG ATTTTGTAGC 201 GGTTGCGGAT TTCGTCGGTA ATCAACACGC CCTGAGCCGC CGTCAGACGG 251 TAGCGGGCAA TGTCGCAGCA AAGGCGCACC AAGATGGGCG GCAGATCCTC 301 AAAAGGTCGT CTGAACCGCC CCAGATACGC GTCGATTTCG GCAGTGGCGT 351 CCACCAGCGC GGTTTGTGCG ACCTCGCGGT CAATCAGCCC CTCGTTGTTG 401 CGGTCGGTGA GCTGCAAGAC TTCCAGCTCA CCGAAACGCG CAACCATATC 451 CTCAACCGTC GCGTATGCCA TTACTCGACC GCCTTGCGTT GCAGCATAGG 501 CTCGGCGCAG ATTGCCTTCC ACACCGCTTC GCCGACTTCG GCGCGCTTCA 551 CTTCGCGCCA GCCGCCGTCA AACAGCAGGC CGCCGCGCCA AAATTCTTTG 601 CCGTCTGCGC CGGTACTGAC GAGCATCACA TCGCGGCTGT CCGCCAAAGC 651 GTCGGCGGCA CGTTGCGTAT GCTGCACTTT GAGTTCGGCA AGTTCGGCGG 701 ACAGTGCCTT TTTGTCGTCT TCGGCTTTTT CCAAGGCTGT GGTCAGCATT 751 TCGACATCGT TTCGGGCGGC GGCAAGCTCT GCCTGCACGG CGTCCAATTC 801 GGCTTTGATG TCTTCAAACG ACGGGGCGGC GGTTTCGGCG GTTTCTGGTT 851 TGTTGTTGGT TTTTGCCATG ATGACTCCTT GTTTCAGACG GCGGCGGATT 901 CGCATTTGA This corresponds to the amino acid sequence <SEQ ID 2442; ORF 723>: m723.pep 1 MRPKPRFRRS VIACSISVIT PEHLIFTVYK HΝTVFARGHF FAAIIHAQLH 51 FAFGHSTQQV EHVDFVAVAD FVGΝQHALSR RQTVAGΝVAA KAHQDGRQIL 101 KRSSEPPQIR VDFGSGVHQR GLCDLAVΝQP LWAVGELQD FQLTETRΝHI 151 LΝRRVCHYST ALRCSIGSAQ IAFHTASPTS ARFTSRQPPS ΝSRPPRQΝSL 201 PSAPVLTSIT SRLSAKASAA RCVCCTLSSA SSADSAFLSS SAFSKAWSI 251 STSFRAAASS ACTASΝSALM SSΝDGAAVSA VSGLLLVFAM MTPCFRRRRI 301 RI* a723. seq not found yet a723.pep not found yet g724.seq not found yet g724.pep not found yet The following partial DΝA sequence, shown with its encoded amino acid sequence, was identified in N meningitidis <SEQ ID 2443>: m724.map ATGAGTTTGAGTAAATTGGCGAAAAAAACGGCACAAACTGCTAAAAATATCGGCGAAACC TACTCAAACTCATTTAACCGCTTTTTTTGCCGTGTTTGACGATTTTTATAGCCGCTTTGG a M S L S K L A K K T A Q T A K Ν I G E T CTGCGCGCGGCCTTTCGGGGAAAAATCACGCTGGTGGTGTCGTCCGAGCCGATACAGCGC 61 + + + + + + 120 GACGCGCGCCGGAAAGCCCCTTTTTAGTGCGACCACCACAGCAGGCTCGGCTATGTCGCG a L R A A F R G K I T L V V S S E P I Q R GTGCAGTTGAGCGGCTTGGCCGACGAAACCCTGCAAGACCTTGAACATTTGCAGGAATAC 121 + + + + + + 180 CACGTCAACTCGCCGAACCGGCTGCTTTGGGACGTTCTGGAACTTGTAAACGTCCTTATG a V Q L S G L A D E T L Q D L E H L Q E Y GGCTTTGCCAGCCATCCGCCCGACGGCAGCGAAGCGGTAGTGATACCGCTGGGCGGCAAT 181 + + + + + + 240 CCGAAACGGTCGGTAGGCGGGCTGCCGTCGCTTCGCCATCACTATGGCGACCCGCCGTTA a G F A S H P P D G S E A V V I P L G G Ν ACTTCGCACGGTGTGATTGTGTGCAGCCAGCACGGCAGCTACCGCATCAAAAACCTTAAG TGAAGCGTGCCACACTAACACACGTCGGTCGTGCCGTCGATGGCGTAGTTTTTGGAATTC a T S H G V I V C S Q H G S Y R I K N L K CCCGGCGAGACGGCGATTTTTAATCATGAGGGTGCAAAAATCGTGATTAAGCAAGGCAAA GGGCCGCTCTGCCGCTAAAAATTAGTACTCCCACGTTTTTAGCACTAATTCGTTCCGTTT a P G E T A I F N H E G A K I V I K Q G K ATCATTGAGGCCGATTGCGACGTGTACCGGGTTAACTGCAAACAATACGAGGTTAATGCG TAGTAACTCCGGCTAACGCTGCACATGGCCCAATTGACGTTTGTTATGCTCCAATTACGC a I I E A D C D V Y R V N C K Q Y E V N A GCCACGGATGCCAAATTTAACGCTCCGTTGGTGGAGACCAGTGCAGTGTTGACGGCGCAA CGGTGCCTACGGTTTAAATTGCGAGGCAACCACCTCTGGTCACGTCACAACTGCCGCGTT a A T D A K F N A P L V E T S A V L T A Q GGCCAAATCAACGGCAACGGCGGCATGGCCGTCGAGGGCGGCGACGGAGCCACCTTTAGC CCGGTTTAGTTGCCGTTGCCGCCGTACCGGCAGCTCCCGCCGCTGCCTCGGTGGAAATCG a G Q I N G N G G M A V E G G D G A T F S GGCGATGTTAACCAAACGGGCGGCAGCTTTAACACCGACGGCGACGTGGTGGCCGGCAAT CCGCTACAATTGGTTTGCCCGCCGTCGAAATTGTGGCTGCCGCTGCACCACCGGCCGTTA a G D V N Q T G G S F N T D G D V V A G N ATATCGTTGCGCCAGCACCCGCATACCGACAGCATCGGCGGCAAAACCTTACCGGCGGAA TATAGCAACGCGGTCGTGGGCGTATGGCTGTCGTAGCCGCCGTTTTGGAATGGCCGCCTT a I S L R Q H P H T D S I G G K T L P A E CCGGCATAG 661 669 GGCCGTATC a P A * - Enzymes that do cut : NONE Enzymes that do not cut: BamHI Bglll EcoRI Hindlll Kpnl Ndel Nhel Pstl Sad Sail Smal Sphl Xbal Xhol This corresponds to the amino acid sequence <SEQ ID 2444; ORF 724>: m724.pep 1 MSLSKLAKKT AQTAKNIGET LRAAFRGKIT LVVSSEPIQR VQLSGLADET 51 LQDLEHLQEY GFASHPPDGS EAWIPLGGN TSHGVIVCSQ HGSYRIKNLK 101 PGETAIFNHE GAKIVIKQGK IIEADCDVYR VNCKQYEVNA ATDAKFNAPL 151 VETSAVLTAQ GQINGNGGMA VEGGDGATFS GDVNQTGGSF NTDGDWAGN 201 ISLRQHPHTD SIGGKTLPAE PA* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2445>: a724.seq 1 ATGAGTTTGA GTAAATTGGC GAAAAAAACG GCACAAACTG CTAAAAATAT 51 CGGCGAAACC CTGCGCGCGG CCTTTCGGGG AAAAATCACG CTGGTGGTGT 101 CGTCCGAGCC GATACAGCGC GTGCAGTTGA GCGGCTTGGC CGACGAAACC 151 CTGCAAGACC TTGAACATTT GCAGGAATAC GGCTTTGCCA GCCATCCGCC 201 CGACGGCAGC GAAGCGGTAG TGATACCGCT GGGCGGCAAT ACTTCGCACG 251 GTGTGATTGT GTGCAGCCAG CACGGCAGCT ACCGCATCAA AAACCTTAAG 301 CCCGGCGAGA CGGCGATTTT TAATCATGAG GGTGCAAAAA TCGTGATTAA 351 GCAAGGCAAA ATCATTGAGG CCGATTGCGA CGTGTACCGG GTTAACTGCA 401 AACAATACGA GGTTAATGCG GCCACGGATG CCAAATTTAA CGCTCCGTTG 451 GTGGAGACCA GTGCAGTGTT GACGGCGCAA GGCCAAATCA ACGGCAACGG 501 CGGCATGGCC GTCGAGGGCG GCGACGGAGC CACCTTTAGC GGCGATGTTA 551 ACCAAACGGG CGGCAGCTTT AACACCGACG GCGACGTGGT GGCCGGCAAT 601 ATATCGTTGC GCCAGCACCC GCATACCGAC AGCATCGGCG GCAAAACCTT 651 ACCGGCGGAA CCGGCATAG This corresponds to the amino acid sequence <SEQ ID 2446; ORF 724. a>: a724.pep 1 MSLSKLAKKT AQTAKNIGET LRAAFRGKIT LWSSEPIQR VQLSGLADET 51 LQDLEHLQEY GFASHPPDGS EAWIPLGGN TSHGVIVCSQ HGSYRIKNLK 101 PGETAIFNHE GAKIVIKQGK IIEADCDVYR VNCKQYEVNA ATDAKFNAPL 151 VETSAVLTAQ GQINGNGGMA VEGGDGATFS GDVNQTGGSF NTDGDWAGN 201 ISLRQHPHTD SIGGKTLPAE PA* a724/m724 100.0% identity in 222 aa overlap 10 20 30 40 50 60 a724 . pep MSLSKLAKKTAQTAKNIGETLRAAFRGKITLWSSEPIQRVQLSGLADETLQDLEHLQEY II I I I II I II II II II II II II II I I II II I II II I II II I II II I II II II I I II II II m724 MSLSKLAKKTAQTAKNIGETLRAAFRGKITLWSSEPIQRVQLSGLADETLQDLEHLQEY 10 20 30 40 50 60 70 80 90 100 110 120 a724 . pep GFASHPPDGSEAWIPLGGNTSHGVIVCSQHGSYRIKNLKPGETAIFNHEGAKIVIKQGK M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M m724 GFASHPPDGSEAWIPLGGNTSHGVIVCSQHGSYRIKNLKPGETAIFNHEGAKIVIKQGK 70 80 90 100 110 120 130 140 150 160 170 180 a724 . pep IIEADCDVYRVNCKQYEVNAATDAKFNAPLVETSAVLTAQGQINGNGGMAVEGGDGATFS II I II II II II II II II I II II II II II II I I I I I I II II II II II I I II II I II I II I I m724 IIEADCDVYRVNCKQYEVNAATDAKFNAPLVETSAVLTAQGQINGNGGMAVEGGDGATFS 130 140 150 160 170 180 190 200 210 220 a724. pep GDVNQTGGSFNTDGDWAGNISLRQHPHTDSIGGKTLPAEPAX II II II II II II II II II II II II II II I II II I II II I II I I m724 GDVNQTGGSFNTDGDWAGNISLRQHPHTDSIGGKTLPAEPAX 190 200 210 220 g725. seq not found yet g7 5.pep not found yet The following partial DNA sequence was identified in N meningitidis <SEQ ID 2447>: m725. seq 1 ATGGTGCGCA CGGTTAAAAG CTACAACGGC GAGGCCGACG ATTTGGCGGG 51 GCAAATCCAT ACGCTGCCTG CGGTTTGGGT AACGTATGGC GGCAGCAAAG 101 TTGAGCCTGC CAGCACCGGC GGCGTATGCG GACGTTATCA GGATACCGCC 151 GAATTTGTGG TGATGGTGGC GGCCCGCAAT CTGCGCAACG AGCAGGCGCA 201 GCGGCAAGGC GGCATCGACA GCCGCGAAAT CGGCAGCAAC GATTTAATCC 251 GCGCTGTTCG CCGCCTGCTT GACGGCCAGC GGCTCGGTTT TGCCGATAGC 301 CGCGGCTTGG TGCCCAAAGC GGTGCGCGCG ATTGCCAATC ATGTGCTGGT 351 GCAAAACGCC GCAGTAAGCA TATATGCGGT TGAGTATGCC ATCCGCTTTA 401 ACACCTGCGG GTTGGAAAAT GACCGCTACC CCGAACGCAC CGACAATCCC 451 GACGACCCCA ACCATATCTT TACCAAGTAT CAGGGTACAT TGAGCGAGCC 501 GTGGCCTGAT TTCGAGGGGT TGGACGGCAA AATTTACGAC CCGCAATCCG 551 CCGATGAAAT ACCTGTAAAC CTAACCCTTA AGGATAAGCA ATGA This corresponds to the amino acid sequence <SEQ ID 2448; ORF 725>: m725.pep 1 MVRTVKSYΝG EADDLAGQIH TLPAVWVTYG GSKVEPASTG GVCGRYQDTA 51 EFWMVAARΝ LRΝEQAQRQG GIDSREIGSΝ DLIRAVRRLL DGQRLGFADS 101 RGLVPKAVRA IAΝHVLVQΝA AVSIYAVEYA IRFΝTCGLEΝ DRYPERTDΝP 151 DDPΝHIFTKY QGTLSEPWPD FEGLDGKIYD PQSADEIPVΝ LTLKDKQ* a725. seq not found yet a725.pep not found yet g726.seq not found yet g726.pep not found yet The following partial DNA sequence was identified in N meningitidis <SEQ ID 2449>: m726.seq 1 ATGACCATCT ATTTCAAAAA CGGCTTTTAC GACGACACAT TGGGCGGCAT 51 CCCCGAAGGC GCGGTTGCCG TCCGCGCCGA AGAATACGCC GCCCTTTTGG 101 CAGGACAGGC GCAGGGCGGG CAGATTGCCG CAGATTCCGA CGGCCGCCCC 151 GTTTTAACCC CGCCGCGCCC GTCCGATTAC CACGAATGGG ACGGCAAAAA 201 ATGGAAAATC AGCAAAGCCG CCGCCGCCGC CCGTTTCGCC AAACAAAAAA 251 CCGCCTTGGC ATTCCGCCTC GCGGAAAAGG CGGACGAACT CAAAAACAGC 301 CTCTTGGCGG GCTATCCCCA AGTGGAAATC GACAGCTTTT ACAGGCAGGA 351 AAAAGAAGCC CTCGCGCGGC AGGCGGACAA CAACGCCCCG ACCCCGATGC 401 TGGCGCAAAT CGCCGCCGCA AGGGGCGTGG AATTGGACGT TTTGATTGAA 451 AAAGTTATCG AAAAATCCGC CCGCCTGGCT GTTGCCGCCG GCGCGATTAT 501 CGGAAAGCGT CAGCAGCTCG AAGACAAATT GAACACCATC GAAACCGCGC 551 CCGGATTGGA CGCGCTGGAA AAGGAAATCG AAGAATGGAC GCTAAACATC 601 GGCTGA This corresponds to the amino acid sequence <SEQ ID 2450; ORF 726>: m726.pep 1 MTIYFKΝGFY DDTLGGIPEG AVAVRAEEYA ALLAGQAQGG QIAADSDGRP 51 VLTPPRPSDY HEWDGKKWKI SKAAAAARFA KQKTALAFRL AEKADELKΝS 101 LLAGYPQVEI DSFYRQEKEA LARQADΝΝAP TPMLAQIAAA RGVELDVLIE 151 KVIEKSARLA VAAGAIIGKR QQLEDKLΝTI ETAPGLDALE KEIEEWTLΝI 201 G* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2451>: a726.seq 1 ATGACCATCT ATTTCAAAAA CGGCTTTTAC GACGACACCT TGGGCAGCAT 51 CCCCGAAGGC GCGGTTGCCG TCCGCGCCGA AGAATACGCC GCCCTTTTGG 101 CAGGACAGGC GCAGGGCGGG CAGATTGCCG CAGATTCCGA CGGCCGCCCC 151 GTTTTAACCC CGCCGCGCCC GTCCGAATAC CACGAATGGG ACGGCAAGAA 201 ATGGGAAATC GGCGAAGCCG CTGCCGCCGC CCGTTTCGCC GAACAAAAAA 251 CCGCCACGGC ATTCCGCCTC GCGGCAAAGG CGGACGAACT CAAAAACAGC 301 CTCTTGGCGG GCTATCCCCA AGTGGAAATC GACAGCTTTT ACAGGCAGGA 351 AAAAGAAGCC CTCGCGCGGC AGGCGGACAA CAACGCCCCG ACCCCGATGC 401 TGGCGCAAAT CGCCGCCGCA AGGGGCGTGG AATTGGACGT TTTGATTGAA 451 AAAGTTGTCG AAAAATCCGC CCGCCTGGCC GTTGCCGCCG GCGCGATTAT 501 CGGAAAGCGG CAGCAGCTCG AAGACAAATT GAACACCATC GAAACCGCGC 551 CAGGATTGGA CGCGCTGGAA AAGGAAATCG AAGAATGGAC GCTAAACATC 601 GGCTGA This corresponds to the amino acid sequence <SEQ ID 2452; ORF 726. a>: a726.pep 1 MTIYFKΝGFY DDTLGSIPEG AVAVRAEEYA ALLAGQAQGG QIAADSDGRP 51 VLTPPRPSEY HEWDGKKWEI GEAAAAARFA EQKTATAFRL AAKADELKΝS 101 LLAGYPQVEI DSFYRQEKEA LARQADΝΝAP TPMLAQIAAA RGVELDVLIE 151 KWEKSARLA VAAGAIIGKR QQLEDKLΝTI ETAPGLDALE KEIEEWTLΝI 201 G* a726/m726 95.5% identity in 201 aa overlap 10 20 30 40 50 60 a726.pep MTIYFKΝGFYDDTLGSIPEGAVAVRAEEYAALLAGQAQGGQIAADSDGRPVLTPPRPSEY II I II II I I I I II IM II II I I II II I II II I II II II I I I I I I I II I II II I I I II I : I m726 MTIYFKΝGFYDDTLGGIPEGAVAVRAEEYAALLAGQAQGGQIAADSDGRPVLTPPRPSDY 10 20 30 40 50 60 70 80 90 100 110 120 a726. pep HEWDGKKWEIGEAAAAARFAEQKTATAFRLAAKADELKNSLLAGYPQVEIDSFYRQEKEA II II II I M M II II II M II II II I I I I I II II I II II II I II II II I II I II II m726 HEWDGKKWKISKAAAAARFAKQKTALAFRLAEKADELKNSLLAGYPQVEIDSFYRQEKEA 70 80 90 100 110 120 130 140 150 160 170 180 a726. pep LARQADNNAPTPMLAQIAAARGVELDVLIEKWEKSARLAVAAGAIIGKRQQLEDKLNTI II II II I II I II I II II II I II II II I I I I I I : I II II I II I II II II II I II II I II II m726 LARQADNNAPTPMLAQIAAARGVELDVLIEKVIEKSARLAVAAGAIIGKRQQLEDKLNTI 130 140 150 160 170 180 190 200 a726.pep ETAPGLDALEKEIEEWTLNIGX II II II I I II I II II II I II II m726 ETAPGLDALEKEIEEWTLNIGX 190 200 g727.seq not found yet g727.pep not found yet The following partial DNA sequence was identified in N meningitidis <SEQ ID 2453>: m727.seq 1 ATGAATCTCG TGAAACTGCT GGCGAATAAC TGGCAACCGA TTGCCATTAT 51 CGCGCTTGTC GGCACGGGCT TGGCTGTGTC GCACCATCAA GGCTACAAGT 101 CGGCATTTGC GAAGCAGCAG GCGGTCATCG ACAAGATGGA GCGCGACAAG 151 GCGCAAGCCC TGCTGTTGTC GGCTCAAAAC TATGCGCGCG AACTGGAACT 201 GGCACGCGCG GAAGCTAAAA AATATGAAGT CAAGGCGCAC GCTGTCGGCA 251 TGGCTTTGGC GAAAAAACAG GCGGAAGTCA GCCGTCTGAA AACGGAAAGA 301 GACCTTTGCA AAATTCCTTT CCCTCCCGAC AGCCGAAACC CAAACACAGG 351 TTTTCGGCTG TTTTCGCCCC AAATACCGCC TAATTTTACC CAAATACCCC 401 CTTAA This corresponds to the amino acid sequence <SEQ TD 2454; ORF 727>: m727.pep 1 MΝLVKLLAΝΝ WQPIAIIALV GTGLAVSHHQ GYKSAFAKQQ AVIDKMERDK 51 AQALLLSAQΝ YARELELARA EAKKYEVKAH AVGMALAKKQ AEVSRLKTER 101 DLCKIPFPPD SRΝPΝTGFRL FSPQIPPΝFT QIPP* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2455>: a727. seq 1 ATGAATCTCG TGAAACTGCT GGCGAATAAC TGGCAACCGA TTGCCATCAT 51 CGCGCTTGTC GGCACGGGTT TGGCGGTGTC GCACCATCAA GGCTACAAGT 101 CGGCTTTTGC GAAGCAGCAG GCGGTCATTG AGAAAATGAA GCGCGACAAG 151 GCGCAAGCCC TGCTGTTGTC GGCTCAAAAC TACGCCCGCG AACTGGAACA 201 GGCGCGTGCG GAAGCTAAAA AATATGAAGT CAAGGCGCAC GCCGTCGGCA 251 TGGCTTTGGC GAAAAAACAG GCGGAAGTCA GCCGTCTGAA AACGGAAAAT 301 AAAAAGGAAA TCGAAAATGT CCTTACTCAA GACCGTAAAA ATGCAGGCGG 351 CGGTTGTATT GACGGCTTTG GCCATCACGG CTTGCAGCTC TACAAGCGCG 401 CCCTCGGCTA CGGAAATTAA This corresponds to the amino acid sequence <SEQ ID 2456; ORF 727.a>: a727.pep 1 MΝLVKLLAΝΝ WQPIAIIALV GTGLAVSHHQ GYKSAFAKQQ AVIEKMKRDK 51 AQALLLSAQΝ YARELEQARA EAKKYEVKAH AVGMALAKKQ AEVSRLKTEΝ 101 KKEIEΝVLTQ DRKΝAGGGCI DGFGHHGLQL YKRALGYGΝ* a727/m727 83.2% identity in 119 aa overlap 10 20 30 40 50 60 a727. pep MΝLVKLLAΝΝWQPIAIIALVGTGLAVSHHQGYKSAFAKQQAVIEKMKRDKAQALLLSAQΝ I II II I I II I II II I II I I I I I I II I I II I II II II I I I II I I: I I : I I I II I M I I M I m727 MNLVKLLANNWQPIAIIALVGTGLAVSHHQGYKSAFAKQQAVIDKMERDKAQALLLSAQN 10 20 30 40 50 60 70 80 90 100 110 119 a727.pep YARELEQARAEAKKYEVKAHAVGMALAKKQAEVSRLKTENKKEIENV-LTQDRKNAGGGC llllll II II I II II II II II II I II I II I I II II I I I : : : : : : I M : I m727 YARELELARAEAKKYEVKAHAVGMALAKKQAEVSRLKTE--RDLCKIPFPPDSRNPNTGF 70 80 90 100 110 120 130 140 a727.pep IDGFGHHGLQLYKRALGYGNX m727 RLFSPQIPPNFTQIPPX 120 130 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2457>: g728.seq 1 ATGTTTAAAA AATTCAAACC GGTACTGTTG TCATTTTTTG CACTTGTATT 51 TGCCTTTTGG CTGGGAACAG GTATTGCCTA TGAGATTAAT CCGCGTTGGT 101 TTTTGAGCGA TACGGCAACT GAAGTACCTG AAAATCCGAA TGCTTTTGTG 151 GCGAAACTTG CCCGCCTGTT CCGAAATGCC GACAGGGCGG TTGTCATCGT 201 GAAGGAATCG ATGAGGACGG AGGAAAGCCT TGCCGGAGCT GTGGATGACG 251 GTCCGTTGCA GTCGGAGAAG GATTATCTCG CGCTCGCTAT CCGGCTCAGT 301 CGTTTGAAAG AAAAGGCGAA ATGGTTTCAC GTAACGGAGC AGGAACATGG 351 GGAAGAGGTT TGGCTGGATT ACTATATCGG CGAGGGCGGT TTGGTTGCGG 401 TTTCGCTTTC GCAACGCTCG CCGGAAGCGT TTGTTAATGC CGAATATCTG 451 TATCGGAACG ATCGTCCGTT TTCTGTAAAT GTGTACGGCG GAACGGCTCA 501 CGGGGAAAAT TATGAAACGA CAGGAGAATA TCGGGTTGTT TGGCAACCGG 551 ACGGTTCGGT ATTTGATGCG GCGGGGCGCG GGAAAATCGG GGAAGATGTT 601 TATGAGCATT GCCTCGGGTG TTATCAGATG GCCCAGGTAT ATTTGGCGAA 651 ATACCGGGAT GTCGCGAATG ACGAGCAGAA GGTTTGGGAC TTCCGCGAAG 701 AGAGCAACCG GATTGCATCG GACTCGCGCG ATTATGTGTT TTATCAGAAT 751 ATGCGGGAAT TGATGCCCCG GGGGATGAAG GCGAACAGTC TTGTGGTCGG 801 CTATGATGCG GACGGTCTGC CGCAAAAAGT CTATTGGAGT TTCGACAATG 851 GAAAAAAACG CCAGAGTTTC GAATATTATT TGAAAAACGG AAATCTTTTT 901 ATTGCACAAT CTTCGACGGT AGCATTGAAA GCGGATGGCG TAACGGCGGA 951 TATGCAGACC TATCATGCGC AACAGACGTG GTATTTGGAT GGCGGGCGGA 1001 TTATCCGCGA AGAGAAACAG GGAGACAGAC TGCCTGATTT TCCTTTGAAC 1051 TTGGAAGATT TGGAAAAAGA GGTGAGCCGT TATGCAGAGG CTGCGGCGAG 1101 ACGTTCGGGC GGCAGGCGCG GCCTTTCTCA CTGA This corresponds to the amino acid sequence <SEQ ID 2458; ORF 728>: g728.pep 1 MFKKFKPVLL SFFALVFAFW LGTGIAYEIΝ PRWFLSDTAT EVPEΝPΝAFV 51 AKLARLFRΝA DRAVVIVKES MRTEESLAGA VDDGPLQSEK DYLALAIRLS 101 RLKEKAKWFH VTEQEHGEEV WLDYYIGEGG LVAVSLSQRS PEAFVΝAEYL 151 YRΝDRPFSVΝ VYGGTAHGEΝ YETTGEYRW WQPDGSVFDA AGRGKIGEDV 201 YEHCLGCYQM AQVYLAKYRD VAΝDEQKVWD FREESΝRIAS DSRDYVFYQΝ 251 MRELMPRGMK AΝSLWGYDA DGLPQKVYWS FDΝGKKRQSF EYYLKΝGΝLF 301 IAQSSTVALK ADGVTADMQT YHAQQTWYLD GGRIIREEKQ GDRLPDFPLΝ 351 LEDLEKEVSR YAEAAARRSG GRRGLSH* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2459>: m728.seq 1 ATGTTTAAAA AATTCAAACC GGTACTGTTG TCATTTTTTG CACTTGTATT 51 TGCCTTTTGG CTGGGAACGG GTATTGCCTA TGAGATTAAT CCGCGTTGGT 101 TTTTGAGCGA TACGGCAACT GAAGTACCTA AAAATCCGAA TGCTTTTGTG 151 GCGAAACTTG CCCGCCTGTT CCGAAATGCC GACAGGGCGG TTGTCATCGT 201 GAAGGAATCG ATAAGGACGG AGGAAAATCT TGCCGGAACT GTGGATGACG 251 GTCCGTTGCA GTCGGAGAAG GATTATCTCG CGCTCGCTAT CCGGCTCAGT 301 CGTTTGAAAG AAAAGGCGAA ATGGTTTCAC GTAACGGAGC AGGAACATGG 351 GAAAGAGGTT TGGCTGGATT ACCATATCGG CGAGGGCGGT TTGGTTGCGG 401 TTTCGCTTTC GCAACGCTCG CCGGAAGCAT TTGTTAATGC CGAATATCTG 451 TATCGGAACG ATCGTCCGTT TTCTGTAAAT GTGTACGGCG GAACGGTTCA 501 CGGGGAAAAT TATGAAACGA CAGGAGAATA TCGGGTTGTT TGGCAACCAG 551 ACGGTTCGGT ATTTGATGCG GCGGGGCGCG GGAAAATCGG GGAAGATGTT 601 TATGAGCATT GCCTCGGGTG TTATCAGATG GCCCAGGTAT ATTTGGCGAA 651 ATACCGGGAT GTCGCGAATG ACGAGCAGAA GGTTTGGGAC TTCCGCAAAG 701 AGAGCAACCG AATTGCGTCG GACTCGCGCA ATTCTGTGTT TTATCAGAAT 751 ATGCGGGAAT TGATGCCCCG AGGGATGAAG GCGAACAGTC TTGTGGTCGG 801 CTATGATGCG GACGGTCTGC CGCAAAAAGT CTATTGGAGT TTCGACAATG 851 GAAAAAAACG CCAGAGTTTC GAATATTATT TGAAAAACGG AAATCTTTTT 901 ATTGCACAAT CTTCGACGGT AGCATTGAAA GCGGATGGCG TAACGGCGGA 951 TATGCAGACC TATCATGCGC AACAGACGTG GTATTTGGAT GGCGGGCGGA 1001 TTGTCCGCGA AGAGAAACAG GGAGACAGAC TGCCTGATTT TCCTTTGAAC 1051 TTGGAAAATT TGGAAAAAGA GGTGCGCCGT TATGCAGAGG CTGCGGCGAG 1101 ACGTTCGGGC GGCAGGCGCG ACCTTTCTCA CTGA This corresponds to the amino acid sequence <SEQ ID 2460; ORF 728>: m728.pep 1 MFKKFKPVLL SFFALVFAFW LGTGIAYEIN PRWFLSDTAT EVPKNPNAFV 51 AKLARLFRNA DRAVVIVKES IRTEENLAGT VDDGPLQSEK DYLALAIRLS 101 RLKEKAKWFH VTEQEHGKEV WLDYHIGEGG LVAVSLSQRS PEAFVNAEYL 151 YRNDRPFSVN VYGGTVHGEN YETTGEYRW WQPDGSVFDA AGRGKIGEDV 201 YEHCLGCYQM AQVYLAKYRD VANDEQKVWD FRKESNRIAS DSRNSVFYQN 251 MRELMPRGMK ANSLWGYDA DGLPQKVYWS FDNGKKRQSF EYYLKNGNLF 301 IAQSSTVALK ADGVTADMQT YHAQQTWYLD GGRIVREEKQ GDRLPDFPLN 351 LENLEKEVRR YAEAAARRSG GRRDLSH* Computer analysis of the amino acid sequences gave the following results: Homology with a predicted ORF from N meningitidis menA with menB ORF 728 shows 96.3% identity over a 377 aa overlap with a predicted ORF (ORF728.a) from N gonorrhoeae: m728 / g728 10 20 30 40 50 60 m728.pep MFKKFKPVLLSFFALVFAFWLGTGIAYEIΝPRWFLSDTATEVPKΝPΝAFVAKLARLFRΝA I I I I I II II I II II I II II II II II II I I II II II II II II II : II II II I II II II II I g 728 MFKKFKPVLLSFFALVFAFWLGTGIAYEIΝPRWFLSDTATEVPEΝPΝAFVAKLARLFRΝA 10 20 30 40 50 60 70 80 90 100 110 120 m728.pep DRAVVIVKESIRTEEΝLAGTVDDGPLQSEKDYLALAIRLSRLKEKAKWFHVTEQEHGKEV II I I II II II M I I M II M II II II II II II I II II I II II I I II II II I I II II M II g728 DRAVVIVKESMRTEESLAGAVDDGPLQSEKDYLALAIRLSRLKEKAKWFHVTEQEHGEEV 70 80 90 100 110 120 130 140 150 160 170 180 m728.pep WLDYHIGEGGLVAVSLSQRSPEAFVΝAEYLYRΝDRPFSVΝVYGGTVHGEΝYETTGEYRW II I M II II I II II I II II II I II II II II II I I II II II I II I M II I II II II II I II g728 WLDYYIGEGGLVAVSLSQRSPEAFVΝAEYLYRΝDRPFSVΝVYGGTAHGEΝYETTGEYRW 130 140 150 160 170 180 190 200 210 220 230 240 m728.pep WQPDGSVFDAAGRGKIGEDVYEHCLGCYQMAQVYLAKYRDVAΝDEQKVWDFRKESΝRIAS I I I I II I I II I II II II I II II II II II II II II II II II II II I II II II M II I I I II g728 WQPDGSVFDAAGRGKIGEDVYEHCLGCYQMAQVYLAKYRDVAΝDEQKVWDFREESΝRIAS 190 200 210 220 230 240 250 260 270 280 290 300 m728.pep DSRΝSVFYQΝMRELMPRGMKAΝSLWGYDADGLPQKVYWSFDΝGKKRQSFEYYLKΝGΝLF MM II I II II II II II II I II II II II II II II II I II II I II I II I II II II I I I II g 728 DSRDYVFYQΝMRELMPRGMKAΝSLWGYDADGLPQKVYWSFDΝGKKRQSFEYYLKΝGΝLF 250 260 270 280 290 300 310 320 330 340 350 360 m728.pep IAQSSTVALKADGVTADMQTYHAQQTWYLDGGRIVREEKQGDRLPDFPLΝLEΝLEKEVRR II II II I I II I II II II I II II I II II I I I I II I : II II II II II I I II II I : I I I I I I g728 IAQSSTVALKADGVTADMQTYHAQQTWYLDGGRIIREEKQGDRLPDFPLNLEDLEKEVSR 310 320 330 340 350 360 370 m728.pep YAEAAARRSGGRRDLSHX I II II II I II II I llll g728 YAEAAARRSGGRRGLSHX 370 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2461>: a728.seq 1 ATGTTTAAAA AATTCAAACC GGTACTGTTG TCATTTTTTG CACTTGTATT 51 TGCCTTTTGG CTGGGAACGG GTATTGCCTA TGAGATTAAT CCGCGTTGGT 101 TTTTGAGCGA TACGGCAACT GAAAATCCGA ATGCTTTTGT GGCGAAACTT 151 GCCCGCCTGT TCCGAAATGC CGACAGGGCG GTTGTCATCG TGAAGGAATC 201 GATGAGGACG GAGGAAAGTC TTGCCGGAGC TGTGGATGAC GGTCCGTTGC 251 AGTCGGAGAA GGATTATCTT GCACTCGCTG TCCGGCTCAG TCGTTTGAAA 301 GAAAAGGCGA AATGGTTTCA CGTAACGGAG CAGGAACATG GGGAAGAGGT 351 TTGGCTGGAT TACTATATCG GCGAGGGCGG TTTGGTTGCG GTTTCGCTTT 401 CGCAACGCTC GCCGGAAGCG TTTGTTAATG CCGAATATCT GTATCGGAAC 451 GATCGTCCGT TTTCTGTAAA TGTGTACGGC GGAACGGTTC ACGGGGAAAA 501 TTATGAAACG ACAGGAGAAT ATCGGGTTGT TTGGCAACCG GACGGTTCGG 551 TATTTGATGC GTCGGGGCGC GGGAAAATCG GGGAAGATGT TTATGAGCAT 601 TGCCTCGGGT GTTATCAGAT GGCCCAGGTA TATTTGGCGA AATATCGGGA 651 TGTCGCGAAT GATGAGCAGA AGGTTTGGGA CTTCCGCGAA GAGAGTAACC 701 GGATTGCGTC GGACTCGCGC GATTCTGTGT TTTATCAGAA TATGCGGGAA 751 TTGATGCCCC GAGGGATGAA GGCAAACAGT CTTGTGGTCG GCTATGATGC 801 GGACGGTCTG CCGCAGAAAG TCTATTGGAG TTTCGACAAT GGGAAAAAAC 851 GCCAGAGTTT CGAATATTAT TTGAAAAACG GAAATCTTTT TATTGCACAA 901 TCTTCGACGG TAGCATTGAA AGCGGATGGC GTAACGGCGG ATATGCAGAC 951 CTATCATGCG CAACAGACGT GGTATTTAGA TGGCGGGCGG ATTGTCCGCG 1001 AAGAGAAACA GGGGGACAGA CTGCCTGATT TTCCTTTGAA CTTGGAAGAT 1051 TTGGAAAAAG AGGTGAGCCG TTATGCAGAG GCTGCGGCGA GACGTTCGGG 1101 CGGCAGGCGC GACCTTTCTC ACTGA This corresponds to the amino acid sequence <SEQ ID 2462; ORF 728.a>: a728.pep 1 MFKKFKPVLL SFFALVFAFW LGTGIAYEIΝ PRWFLSDTAT EΝPΝAFVAKL 51 ARLFRΝADRA WIVKESMRT EESLAGAVDD GPLQSEKDYL ALAVRLSRLK 101 EKAKWFHVTE QEHGEEVWLD YYIGEGGLVA VSLSQRSPEA FVΝAEYLYRΝ 151 DRPFSVΝVYG GTVHGEΝYET TGEYRWWQP DGSVFDASGR GKIGEDVYEH 201 CLGCYQMAQV YLAKYRDVAΝ DEQKVWDFRE ESΝRIASDSR DSVFYQΝMRE 251 LMPRGMKAΝS LWGYDADGL PQKVYWSFDΝ GKKRQSFEYY LKΝGΝLFIAQ 301 SSTVALKADG VTADMQTYHA QQTWYLDGGR IVREEKQGDR LPDFPLΝLED 351 LEKEVSRYAE AAARRSGGRR DLSH* a728 / m728 96.3% identity in 377 aa overlap 10 20 30 40 50 a728.pep MFKKFKPVLLSFFALVFAFWLGTGIAYEIΝPRWFLSDTATE ΝPΝAFVAKLARLFRΝA II II I II II I II II I I II I II II II I II II I I II II II I II I I II II I II II II I I I m728 MFKKFKPVLLSFFALVFAFWLGTGIAYEIΝPRWFLSDTATEVPKΝPΝAFVAKLARLFRΝA 10 20 30 40 50 60 60 70 80 90 100 110 a728 . pep DRAWIVKESMRTEESLAGAVDDGPLQSEKDYLALAVRLSRLKEKAKWFHVTEQEHGEEV I I I I I I I I I I : I I I I : I I M I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I : I I m728 DRAVVIVKES IRTEEΝLAGTVDDGPLQSEKDYLALAIRLSRLKEKAKWFHVTEQEHGKEV 70 80 90 100 110 120 120 130 140 150 160 170 a728 . pep WLDYYIGEGGLVAVSLSQRSPEAFVΝAEYLYRΝDRPFSVΝVYGGTVHGEΝYETTGEYRW I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m728 WLDYHIGEGGLVAVSLSQRSPEAFVΝAEYLYRΝDRPFSVΝVYGGTVHGEΝYETTGEYRVV 130 140 150 160 170 180 180 190 200 210 220 230 a728. pep WQPDGSVFDASGRGKIGEDVYEHCLGCYQMAQVYLAKYRDVANDEQKVWDFREESNRIAS II II II I II I : I II I I II I I II II I I II I II II I II I I II II I I I I I I I II I : I I II I I I m728 WQPDGSVFDAAGRGKIGEDVYEHCLGCYQMAQVYLAKYRDVANDEQKVWDFRKESNRIAS 190 200 210 220 230 240 240 250 260 270 280 290 a728.pep DSRDSVFYQNMRELMPRGMKANSLWGYDADGLPQKVYWSFDNGKKRQSFEYYLKNGNLF II I : I II II II II II I I I I I II I II I II I II II II II II II II II I II I II I I II II I II m728 DSRNSVFYQNMRELMPRGMKANSLWGYDADGLPQKVYWSFDNGKKRQSFEYYLKNGNLF 250 260 270 280 290 300 300 310 320 330 340 350 a728. pep IAQSSTVALKADGVTADMQTYHAQQTWYLDGGRIVREEKQGDRLPDFPLNLEDLEKEVSR II I II I II II II II I II I II II II I II II II II I II II II II II I I II I I II : II II I I m728 IAQSSTVALKADGVTADMQTYHAQQTWYLDGGRIVREEKQGDRLPDFPLNLENLEKEVRR 310 320 330 340 350 360 360 370 a728.pep YAEAAARRSGGRRDLSHX I II II I II I II I II II I I m728 YAEAAARRSGGRRDLSHX 370 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 2463>: g729.seq 1 ATGAATACTA CATTGAAAAC TACCTTGACC TCTGTTGCAG CAGCCTTTGC 51 ATTGTCTGCC TGCACCATGA TTCCTCAATA CGAGCAGCCC AAAGTCGAAG 101 TTGCGGAAAC CTTCCAAAAC GACACATCGG TTTCTTCCAT CCGCGCGGTT 151 GATTTGGGTT GGCATGACTA TTTTGCCGAC CCGCGCCTGC AAAAGCTGAT 201 CGACATCGCA CTCGAGCGCA ATACCAGTTT GCGTACAGCC GTATTGAACA 251 GCGAAATCTA CCGCAAACAA TACATGATCG AGCGCAACAA CCTCCTGCCC 301 ACGCTTGCCG CCAATGCGAA CGGCTCGCGC CAAGGCAGCT TGAGCGGCgg 351 caaTGTCAGC AGCAGCTACA ATGTCGGACT GGGTGcGGca tCTTACGAAC 401 TCGATCTGTT CgGGCGCGTG CGCagcaacA GcgaagcAGC ACTGcaggGC 451 tATTTTGCCA GCGTTGCCAA CcgcGATGCG GCACATTTGa ttCtGATTGC 501 CACCGTTGCC AAAGCCTATT TCAAcgaGcG TTATGCCGAA AAAGcgatgT 551 CTTTGGCGCa gcGTGTCTTG AAAACGCGCG AGGAAACCTA CAAGCTGTCC 601 GAATTGCGGT ACAAGGCAGG CGTGATTTCC GCCGTCGCCC TGCGCCAGCA 651 GGAAGCCTTG ATTGAATCTG CCAAAGCCGA TTATGCCCAT GCCGCGCGCa 701 gcCGCGAACA GGCGCGCAAT GCCTTGGCAA CCTTGATTAA ccGTCCGATA 751 CCCGAagaCC TGCCCGCCGG TTTGCCGTTG GACAagcAGT TTTTTGTTGA 801 AAAACTGCCT GCCGGTTTGA GTTCCGAAGT ATTGCTCGAC CGTCCCGACA 851 TCCGCGCCGC CGAACACGCG CTCAAACAGG CAAACGCCAA TATCGGTGCG 901 gcgCGCGCCg ccTTTTTCCC GTCCATCCGC CTGACCGGAA GCGTCGGTAC 951 GGGTTCTGTC GAATTGGGCG GGCTGTTCAA AAGCGGCACG GGCGTTTGGG 1001 CGTTCGCTCC GTCTATTACC CTGCCGATTT TTACTTGGGG AACGAACAAG 1051 GCGAACCTTG ATGTGGCAAA ACTGCGCCAA CAGGCACAAA TTGTTGCCTA 1101 TGAATCCGCC GTCCAATCCG CCTTTCAAGA CGTGGCAAAC GCATTGGCGG 1151 CGCGCGAGCA GCTGGATAAA GCCTATGACG CTTTAAGCAA ACAAAGCCGC 1201 GCCTCTAAAG AAGCGTTGCG CTTGGTCGGA CTGCGTTACA AACACGGCGT 1251 ATCCGGCGCG CTCGATTTGC TCGATGCGGA ACGCATCAGC TATTCGGCGG 1301 AAGGTGCGGC TTTGTCGGCA CAACTGACCC GCGCCGAAAA CCTTGCCGAT 1351 TTGTACAAGG CGCTCgacGG CGGATTGAAA CGGGATACCC AAACCGGCAA 1401 ATAA This corresponds to the amino acid sequence <SEQ ID 2464; ORF 729>: g729.pep 1 MΝTTLKTTLT SVAAAFALSA CTMIPQYEQP KVEVAETFQΝ DTSVSSIRAV 51 DLGWHDYFAD PRLQKLIDIA LERΝTSLRTA VLΝSEIYRKQ YMIERΝΝLLP 101 TLAAΝAΝGSR QGSLSGGΝVS SSYΝVGLGAA SYELDLFGRV RSΝSEAALQG 151 YFASVAΝRDA AHLILIATVA KAYFΝERYAE KAMSLAQRVL KTREETYKLS 201 ELRYKAGVIS AVALRQQEAL IESAKADYAH AARSREQARN ALATLINRPI 251 PEDLPAGLPL DKQFFVEKLP AGLSSEVLLD RPDIRAAEHA LKQANANIGA 301 ARAAFFPSIR LTGSVGTGSV ELGGLFKSGT GVWAFAPSIT LPIFTWGTNK 351 ANLDVAKLRQ QAQIVAYESA VQSAFQDVAN ALAAREQLDK AYDALSKQSR 401 ASKEALRLVG LRYKHGVSGA LDLLDAERIS YSAEGAALSA QLTRAENLAD 451 LYKALDGGLK RDTQTGK* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2465>: m729.seq 1 ATGGATACTA CATTGAAAAC CACCTTGACT TCTGTTGCAG CAGCCTTTGC 51 ATTGTCTGCC TGCACCATGA TTCCCCAATA CGAGCAGCCC AAAGTCGAAG 101 TTGCCGAAAC GTTCAAAAAC GATACCGCCG ACAGCGGCAT CCGCGCCGTC 151 GATTTAGGTT GGCATGACTA TTTTGCCGAC CCGCGCCTGC AAAAGCTGAT 201 CGACATCGCA CTCGAGCGCA ATACCAGTTT GCGTACCGCC GTATTGAACA 251 GCGAAATCTA CCGCAAACAA TACATGATTG AGCGCAACAA CCTCCTGCCC 301 ACGCTTGCCG CCAATGCGAA CGACTCGCGC CAAGGCAGCT TGAGCGGCGG 351 CAATGTAAGC AGCAGCTACA AAGTCGGACT GGGTGCGGCA TCTTACGAAC 401 TCGATCTGTT CGGGCGTGTA CGCAGCAGCA GCGAGGCGGC ACTGCAAGGC 451 TATTTCGCCA GCACCGCCAA CCGCGATGCG GCACATTTGA GCCTGATTGC 501 CACCGTTGCC AAAGCCTATT TCAACGAACG TTACGCCGAA GAAGCGATGT 551 CTTTGGCGCA ACGTGTTTTG AAAACGCGCG AGGAAACCTA CAAGCTGTCC 601 GAATTACGTT ACAAGGCAGG CGTGATTTCC GCCGTCGCCC TACGTCAGCA 651 GGAAGCCCTG ATCGAATCTG CCAAAGCCGA TTATGCCCAT GCCGCGCGCA 701 GCCGCGAACA GGCGCGCAAT GCCTTGGCAA CCTTGATTAA CCAACCGATA 751 CCCGAAGACC TGCCTGCCGG TTTGCCGCTG GACAAGCAGT TTTTTGTTGA 801 AAAACTGCCG GCCGGTTTGA GTTCCGAAGT ATTGCTCGAC CGTCCCGATA 851 TCCGTGCTGC CGAACACGCG CTCAAACAGG CAAACGCCAA TATCGGTGCG 901 GCACGCGCCG CCTTTTTCCC ATCCATCCGC CTGACCGGAA CCGTCGGTAC 951 GGGTTCTGCC GAATTGGGTG GGTTGTTCAA AAGCGGCACG GGCGTTTGGT 1001 CGTTCGCGCC GTCTATTACC CTGCCGATTT TTACCTGGGG TACGAACAAG 1051 GCGAACCTTG ATGTAGCCAA GCTGCGCCAA CAGGTACAAA TCGTTGCCTA 1101 TGAATCCGCC GTCCAATCCG CATTTCAAGA CGTGGCAAAC GCATTGGCGG 1151 CGCGCGAGCA GCTGGATAAA GCCTATGACG CTTTAAGCAA ACAAAGCCGC 1201 GCCTCTAAAG AAGCGTTGCG CTTGGTCGGC CTGCGTTACA AGCACGGCGT 1251 ATCCGGCGCG CTCGACTTGC TCGATGCGGA ACGCAGCAGC TATGCGGCGG 1301 AGGGTGCGGC TTTGTCGGCA CAACTGACCC GCGCCGAAAA CCTTGCCGAT 1351 TTGTACAAGG CACTCGGCGG CGGATTGAAA CGGGATACCC AAACCGACAA 1401 ATAA This corresponds to the amino acid sequence <SEQ ID 2466; ORF 729>: m729.pep 1 MDTTLKTTLT SVAAAFALSA CTMIPQYEQP KVEVAETFKΝ DTADSGIRAV 51 DLGWHDYFAD PRLQKLIDIA LERΝTSLRTA VLΝSEIYRKQ YMIERΝΝLLP 101 TLAAΝAΝDSR QGSLSGGΝVS SSYKVGLGAA SYELDLFGRV RSSSEAALQG 151 YFASTAΝRDA AHLSLIATVA KAYFΝERYAE EAMSLAQRVL KTREETYKLS 201 ELRYKAGVIS AVALRQQEAL IESAKADYAH AARSREQARN ALATLINQPI 251 PEDLPAGLPL DKQFFVEKLP AGLSSEVLLD RPDIRAAEHA LKQANANIGA 301 ARAAFFPSIR LTGTVGTGSA ELGGLFKSGT GVWSFAPSIT LPIFTWGTNK 351 ANLDVAKLRQ QVQIVAYESA VQSAFQDVAN ALAAREQLDK AYDALSKQSR 401 ASKEALRLVG LRYKHGVSGA LDLLDAERSS YAAEGAALSA QLTRAENLAD 451 LYKALGGGLK RDTQTDK* Computer analysis of the amino acid sequences gave the following results: Homology with a predicted ORF from N meningitidis menA with menB ORF 729 shows 95.7% identity over a 467 aa overlap with a predicted ORF (ORF729.a) from N gonorrhoeae: m729 / g729 95.7% identity in 467 aa overlap 10 20 30 40 50 60 m729.pep MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKΝDTADSGIRAVDLGWHDYFAD I : M II I II I II II I I II II II II I II I I II II I II I I : II I : I : I II II I II II II I I g729 MΝTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFQΝDTSVSSIRAVDLGWHDYFAD 10 20 30 40 50 60 70 80 90 100 110 120 m729.pep PRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTLAANANDSRQGSLSGGNVS I II I I II II II I II II II II II II II II II II II I I I II II II II II I II II II I I I II g729 PRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTLAANANGSRQGSLSGGNVS 70 80 90 100 110 120 130 140 150 160 170 180 m729. pep SSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDAAHLSLIATVAKAYFNERYAE I I I: II II II II II I II II II I: II II I II I I I I : II II II II II II I II II I II I II I g729 SSYNVGLGAASYELDLFGRVRSNSEAALQGYFASVANRDAAHLILIATVAKAYFNERYAE 130 140 150 160 170 180 190 200 210 220 230 240 m729. pep EAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQEALIESAKADYAHAARSREQARN : II I I II II II II I II II II II II II I I II I II I II II II I II I II II II II II II II II g729 KAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQEALIESAKADYAHAARSREQARN 190 200 210 220 230 240 250 260 270 280 290 300 m729.pep ALATLINQPIPEDLPAGLPLDKQFFVEKLPAGLSSEVLLDRPDIRAAEHALKQANANIGA I I II I II : II II II II II II I II I II II I I I I II II I II II I II I I I I II II I I II I II I g729 ALATLINRPIPEDLPAGLPLDKQFFVEKLPAGLSSEVLLDRPDIRAAEHALKQANANIGA 250 260 270 280 290 300 310 320 330 340 350 360 m729.pep ARAAFFPSIRLTGTVGTGSAELGGLFKSGTGVWSFAPSITLPIFTWGTNKANLDVAKLRQ I I II I I I I II II I : I II II : II II II I I I II I I : I II II II II II II II II I I I II I II I g729 ARAAFFPSIRLTGSVGTGSVELGGLFKSGTGVWAFAPSITLPIFTWGTNKANLDVAKLRQ 310 320 330 340 350 360 370 380 390 400 410 420 m729. pep QVQIVAYESAVQSAFQDVANALAAREQLDKAYDALSKQSRASKEALRLVGLRYKHGVSGA I: II I II II II II I II II II I II II II II II I I II II I II II I I II II II II I I I II I II g729 QAQIVAYESAVQSAFQDVANALAAREQLDKAYDALSKQSRASKEALRLVGLRYKHGVSGA 370 380 390 400 410 420 430 440 450 460 m729.pep LDLLDAERSSYAAEGAALSAQLTRAENLADLYKALGGGLKRDTQTDKX I I II I I II I I: I II I II I I II II II II II II I II I II II II II II g729 LDLLDAERISYSAEGAALSAQLTRAENLADLYKALDGGLKRDTQTGKX 430 440 450 460 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2467>: a729. . seq 1 ATGGATACTA CATTGAAAAC CACCTTGACT TCTGTTGCAG CAGCCTTCGC 51 ATTATCCGCC TGCACCATGA TTCCCCAATA CGAGCAGCCC AAAGTCGAAG 101 TTGCCGAAAC GTTCAAAAAC GATACCGCCG ACAGCGGCAT CCGTGCGGTC 151 GATTTGGGTT GGCATGACTA TTTTGCCGAC CCGCGCCTGC AAAAGCTGAT 201 CGACATCGCA CTCGAGCGCA ATACCAGTTT GCGTACCGCC GTATTGAACA 251 GCGAAATCTA CCGCAAACAA TACATGATTG AGCGCAACAA CCTCCTGCCC 301 ACGCTTGCCG CCAATGCGAA CGACTCGCGC CAAGGCAGCT TGAGCGGCGG 351 CAATGTAAGC AGCAGCTACA AAGTCGGACT GGGTGCGGCA TCTTACGAAC 401 TCGATCTGTT CGGGCGTGTA CGCAGCAGCA GCGAGGCGGC ACTGCAAGGC 451 TATTTCGCCA GCACCGCCAA CCGCGATGCG GCACATTTGA GCCTGATTGC 501 CACCGTTGCC AAAGCCTATT TCAACGAACG TTATGCCGAA GAAGCGATGT 551 CTTTGGCGCA ACGTGTTTTG AAAACGCGCG AGGAAACCTA CAAGCTGTCC 601 GAATTACGTT ACAAGGCAGG CGTGATTTCC GCCGTCGCCC TACGTCAGCA 651 GGAAGCCCTA ATCGAATCTG CCAAAGCCGA TTATGCCCAT GCCGCGCGCA 701 GCCGCGAACA GGCGCGCAAT GCCTTGGCAA CCCTGATTAA CCAACCGATA 751 CCCGACGACC TGCCCGCCGG TTTGCCGTTG GACAAGCAGT TTTTTGTTGA 801 GAAGCTGCCG GCCGGTTTGA GTTCCGAAGT ATTGCTCGAC CGTCCCGATA 851 TCCGTGCTGC CGAACACGCG CTCAAACAGG CAAACGCCAA TATCGGTGCG 901 GCACGCGCCG CCTTTTTCCC ATCCATCCGC CTGACCGGAA GCGTCGATAC 951 GCATTCTGCC GAATTGGGCG GGCTGTTCAA AAGCGGCACC GGCGTTTGGT 1001 TGTTCGCACC TTCCATTACC CTGCCGATTT TTACCTGGGG TACGAACAAG 1051 GCGAACCTCG ATGTAGCCAA GCTGCGCCAA CAGGCACAAA TCGTTGCCTA 1101 TGAAGCCGCC GTCCAATCCG CATTTCAAGA CGTGGCAAAC GCATTGACCG 1151 CGCGCGAGCA GTTGGATAAA GCCTATGACG CTTTAAGCAA ACAAAGCCGC 1201 GCCTCTAAAG AAGCGTTGCG TTTGGTCGGT CTGCGTTACA AACACGGCGT 1251 ATCCGGCGCG CTCGACTTGC TCGATGCGGA ACGCAGCAGC TATTCGGCGG 1301 AAGGTGCGGC TTTGTCGGCA CAACTGACCC GCGCCGAAAA CCTTGCCGAT 1351 TTGTACAAGG CACTCGGCGG CGGATTGAAA CGGGATACCC AAACCGACAA 1401 ATAA This corresponds to the amino acid sequence <SEQ ID 2468; ORF 729.a>: a729.pep 1 MDTTLKTTLT SVAAAFALSA CTMIPQYEQP KVEVAETFKN DTADSGIRAV 51 DLGWHDYFAD PRLQKLIDIA LERNTSLRTA VLNSEIYRKQ YMIERNNLLP 101 TLAANANDSR QGSLSGGNVS SSYKVGLGAA SYELDLFGRV RSSSEAALQG 151 YFASTANRDA AHLSLIATVA KAYFNERYAE EAMSLAQRVL KTREETYKLS 201 ELRYKAGVIS AVALRQQEAL IESAKADYAH AARSREQARN ALATLINQPI 251 PDDLPAGLPL DKQFFVEKLP AGLSSEVLLD RPDIRAAEHA LKQANANIGA 301 ARAAFFPSIR LTGSVDTHSA ELGGLFKSGT GVWLFAPSIT LPIFTWGTNK 351 ANLDVAKLRQ QAQIVAYEAA VQSAFQDVAN ALTAREQLDK AYDALSKQSR 401 ASKEALRLVG LRYKHGVSGA LDLLDAERSS YSAEGAALSA QLTRAENLAD 451 LYKALGGGLK RDTQTDK* a729 / m729 98.1% identity in 467 aa overlap 10 20 30 40 50 60 a729.pep MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTADSGIRAVDLGWHDYFAD I II I II II II II II II II II I I II I I II I II II I II II I II II II II II II II II II I I I m 729 MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTADSGIRAVDLGWHDYFAD 10 20 30 40 50 60 70 80 90 100 110 120 a729.pep PRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTLAANANDSRQGSLSGGNVS II I I I II I II II II I I II II II II II I II II II I II I I I I II I II II II I II I II II I I I πι729 PRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTLAANANDSRQGSLSGGNVS 70 80 90 100 110 120 130 140 150 160 170 180 a729. pep SSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDAAHLSLIATVAKAYFNERYAE II I I I II I II II II II II II II II II I I I II I II II II II II I II II II I II I II II II I m729 SSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDAAHLSLIATVAKAYFNERYAE 130 140 150 160 170 180 190 200 210 220 230 240 a729. pep EAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQEALIESAKADYAHAARSREQARN II I II I II II I II II I I I II II II II I II II I II II II II II I II II I II II II II II II m729 EAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQEALIESAKADYAHAARSREQARN 190 200 210 220 230 240 250 260 270 280 290 300 a729.pep ALATLINQPIPDDLPAGLPLDKQFFVEKLPAGLSSEVLLDRPDIRAAEHALKQANANIGA II I II II I II I : II II II II I II II I II I II II II I I II I II I II II II I I II II II II I m729 ALATLINQPIPEDLPAGLPLDKQFFVEKLPAGLSSEVLLDRPDIRAAEHALKQANANIGA 250 260 270 280 290 300 310 320 330 340 350 360 a729.pep ARAAFFPSIRLTGSVDTHSAELGGLFKSGTGVWLFAPSITLPIFTWGTNKANLDVAKLRQ II II II II II II I : I I I II II II I I II II I I I I II I II I II II II I II II II II II I m729 ARAAFFPSIRLTGTVGTGSAELGGLFKSGTGVWSFAPSITLPIFTWGTNKANLDVAKLRQ 310 320 330 340 350 360 370 380 390 400 410 420 a729. pep QAQIVAYEAAVQSAFQDVANALTAREQLDKAYDALSKQSRASKEALRLVGLRYKHGVSGA M II II II M II II I I M II I M II I I I I II I II I I II II II II I II II I I II II I I II I m729 QVQIVAYESAVQSAFQDVANALAAREQLDKAYDALSKQSRASKEALRLVGLRYKHGVSGA 370 380 390 400 410 420 430 440 450 460 a729.pep LDLLDAERSSYSAEGAALSAQLTRAENLADLYKALGGGLKRDTQTDKX II II I II I II I : I II II II I II II I II II II II II II I II II II II II m729 LDLLDAERSSYAAEGAALSAQLTRAENLADLYKALGGGLKRDTQTDKX 430 440 450 460 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2469>: g730.seq 1 GTGAAACCGC TGCGCAGACT GACAAACCTC CTTGCCGCCT GCGCCGTAGC 51 GGCGGTCGCA CTCATACAGC CCGCCCTCGC GGCGGACTTG GCGCAAGACC 101 CGTTCATTAC CGATAACACC CAACGGCAGC ACTACGAACC CGGCGGCAAA 151 TACCACCTCT TCGGcgaCCC GCGCGGCAGC GTTTCCGACC GCACCGGCAA 201 AATCAACGTC ATCCAAGACT ATACCCACCA GATGGGCAAC CTGCTCATCC 251 AACAGGCGGC AATCCAAGGC AATCTTGGTT ACACCGTCCG CTTTTCCGGA 301 CACGGACACG AAGAACACGC CCCCTTCGAC AACCACGCCG CCGACAGCGC 351 AAGCGAAGAA AAAGGCAACG TTGACGACGG CTTTACCGTG TACCGGCTCA 401 ACTGGGAAGG ACACGAACAT CATCCCGCCG ATGCCTACGA CGGCCCGAAG 451 GGCGGCAATT ACCCCAAACC TACGGGCGCA CGAGACGAAT ACACCTATCA 501 CGTCAACGGC ACAGCCCGCA GTATCAAACT CAATCCGACC GACACCCGCA 551 GCATCCGGCA ACGCATATTC GACAACTACA ACAACCTCGG CAGCAATTTC 601 TCCGACCGCG CCGATGAAGC CAACAGAAAA ATGTTCGAGC ACAATGCCAA 651 GCTCGACCGC TGGGGCAACA GCATGGAGTT TGTCAACGGC GTCGCCGCCG 701 GCGCGCTCAA CCCCTTTATC AGCGCGGGCG AAGCCTTGGG CATAGGCGAC 751 ATACTGTACG GAACGCGCTA TGCCATAGAC AAAGCCGCGA TGCGCAACAT 801 CGCCCCCTTA CCCGCCGAGG GCAAATTCGC CGCCATCGGC GGCTTGGGCA 851 GCGCGGCGGG CTTTGAAAAA AATACGCGCG AAGCCGTTGA CCGGTGGATA 901 CAGGAAAACC CCAATGCCGC CGAAACCGTC GAAGCCCTGG TCAACGTCCT 951 GCCGTTTGCC AAAGTCAAAA ACCTGACAAA GGCGGCAAAA CCGGGGAAGG 1001 CTGCGGTTAG TGGGGATTTT TCTAAATCCT ACACCTGCTC CTTCCACGGC 1051 AGCACCTTGG TCAAAACGGC AGACGGCTAC AAAGCCATTG CCCATATTCA 1101 AGCCGGAGAC CGCGTCCTTT CCAAGGACGA GGCAAGCGGA GAAACGGGAT 1151 ACAAACCCGT TACCGCCCGA TACGGCAATC CGTATCAAGA AACCGTTTAC 1201 ATTGAAGTTT CAGACGGCAT CGGCAACAGC CAAACCCTGA TTTCCAACCG 1251 CATCCACCCG TTTTATTCGG ACGGCAAATG GATTAAGGCG GAAGATTTAA 1301 AAGCGGGAAG CCGGCTGTTA TCCGAAAGCG GCAAAACCCA AACCGTCCGC 1351 AACATCGTTG TCAAACCAAA ACCGCTCAAA GCCTACAATC TGACCGTTGC 1401 CGATTGGCAT ACCTACTTCG TCAAGGGTAA TCAGGCGGAA ACGGAAGGGG 1451 TTTGGGTTCA TAATGATTGT CCGCCTAAAC CAAAACCAAC CAATCATGCC 1501 CAACAAAGAA AAGAAGAAGC TAAAAACGAT TCTCATCGAA GTGTGGGAGA 1551 TTCCAATCGT GTCGTTCGCG AAGGAAAGCA ATATTTAGAT TCCGACACAG 1601 GAAACCATGT TTATGTAAAA GGAGATAAAG TGGTTATTCT AACTCCTGAT 1651 GGAAGACAGG TAACTCAATT TAAGAACTCG AAAGCCAATA CGTCAAAAAG 1701 GGTAAAAAAT GGGAAATGGA CACCAAAATA A This corresponds to the amino acid sequence <SEQ ID 2470; ORF 730.ng>: g730.pep 1 VKPLRRLTΝL LAACAVAAVA LIQPALAADL AQDPFITDΝT QRQHYEPGGK 51 YHLFGDPRGS VSDRTGKIΝV IQDYTHQMGΝ LLIQQAAIQG ΝLGYTVRFSG 101 HGHEEHAPFD ΝHAADSASEE KGΝVDDGFTV YRLΝWEGHEH HPADAYDGPK 151 GGΝYPKPTGA RDEYTYHVΝG TARSIKLΝPT DTRSIRQRIF DΝYΝΝLGSΝF 201 SDRADEAΝRK MFEHΝAKLDR WGΝSMEFVΝG VAAGALΝPFI SAGEALGIGD 251 ILYGTRYAID KAAMRΝIAPL PAEGKFAAIG GLGSAAGFEK ΝTREAVDRWI 301 QEΝPΝAAETV EALVΝVLPFA KVKΝLTKAAK PGKAAVSGDF SKSYTCSFHG 351 STLVKTADGY KAIAHIQAGD RVLSKDEASG ETGYKPVTAR YGΝPYQETVY 401 IEVSDGIGΝS QTLISΝRIHP FYSDGKWIKA EDLKAGSRLL SESGKTQTVR 451 ΝIWKPKPLK AYΝLTVADWH TYFVKGΝQAE TEGVWVHΝDC PPKPKPTΝHA 501 QQRKEEAKΝD SHRSVGDSΝR REGKQYLD SDTGΝHVYVK GDKWILTPD 551 GRQVTQFKΝS KAΝTSKRVKΝ GKWTPK* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2471>: m730. seq 1 GTGAAACCGC TGCGCAGACT GACAAACCTC CTTGCCGCCT GCGCCGTAGC 51 GGCGGCCGCA CTCATACAGC CCGCCCTCGC GGCGGACTTG GCGCAAGACC 101 CGTTCATTAC CGATAACGCC CAACGGCAGC ACTACGAACC CGGCGGCAAA 151 TACCACCTCT TCGGCGACCC GCGCGGCAGC GTTTCCGACC GCACCGGCAA 201 AATCAACGTC ATCCAAGACT ATACCCACCA GATGGGCAAC CTGCTCATCC 251 AACAGGCAAA CATCAACGGC ACAATCGGCT ACCACACCCG CTTTTCCGGA 301 CACGGACACG AAGAACACGC CCCCTTCGAC AACCACGCCG CCGACAGCGC 351 GAGCGAAGAA AAAGGCAACG TTGACGAAGG CTTTACCGTA TACCGGCTCA 401 ACTGGGAAGG ACACGAACAT CATCCCGCCG ATGCCTACGA CGGCCCGAAG 451 GGCGGCAATT ACCCCAAACC TACGGGCGCA CGAGACGAAT ACACCTATCA 501 CGTCAACGGC ACAGCCCGCA GTATCAAACT CAATCCGACC GACACCCGCA 551 GCATCCGGCA ACGCATATCC GACAATTACA GCAACCTCGG CAGCAATTTC 601 TCCGACCGCG CCGATGAAGC CAACAGAAAA ATGTTCGAGC ACAATGCCAA 651 GCTCGACCGC TGGGGCAACA GCATGGAGTT TATCAACGGC GTCGCCGCCG 701 GCGCGCTCAA CCCCTTTATC AGCGCGGGCG AAGCCTTGGG CATAGGCGAC 751 ATACTGTACG GAACGCGCTA TGCCATAGAC AAAGCCGCAA TGCGCAACAT 801 CGCCCCCTTG CCCGCCGAGG GCAAATTCGC CGTCATCGGC GGCTTGGGCA 851 GCGTGGCGGG CTTTGAAAAG AATACGCGCG AAGCCGTTGA CCGGTGGATA 901 CAGGAAAATC CCAATGCCGC CGAAACCGTC GAAGCCGTCT TCAACGTTGC 951 CGCAGCAGCC AAAGTCGCGA AGTTGGCAAA GGCGGCAAAA CCAGGGAAGG 1001 CTGCGGTTAG CGGGGATTTT GCTGATTCTT ATAAAAAGAA ATTGGCTTTG 1051 TCTGATAGTG CGAGACAGTT ATATCAAAAT GCAAAGTATA GAGAAGCTCT 1101 AGATATACAT TATGAAGATT TAATTAGAAG AAAAACTGAT GGTTCATCAA 1151 AATTTATTAA CGGCAGAGAA ATTGACGCTG TTACGAATGA TGCTTTAATA 1201 CAAGCCAAAA GAACAATTTC AGCAATAGAT AAACCTAAAA ATTTCTTAAA 1251 TCAAAAAAAT AGAAAGCAAA TTAAAGCAAC CATCGAAGCA GCAAACCAAC 1301 AGGGAAAACG TGCAGAATTT TGGTTTAAAT ACGGTGTTCA TTCACAAGTT 1351 AAGTCATATA TTGAATCAAA AGGCGGCATT GTTAAAACAG GTTTAGGAGA 1401 TTAA This corresponds to the amino acid sequence <SEQ ID 2472; ORF 730>: m730.pep 1 VKPLRRLTNL LAACAVAAAA LIQPALAADL AQDPFITDNA QRQHYEPGGK 51 YHLFGDPRGS VSDRTGKINV IQDYTHQMGN LLIQQANING TIGYHTRFSG 101 HGHEEHAPFD NHAADSASEE KGNVDEGFTV YRLNWEGHEH HPADAYDGPK 151 GGNYPKPTGA RDEYTYHVNG TARΞIKLNPT DTRSIRQRIS DNYSNLGSNF 201 SDRADEANRK MFEHNAKLDR WGNSMEFING VAAGALNPFI SAGEALGIGD 251 ILYGTRYAID KAAMRNIAPL PAEGKFAVIG GLGSVAGFEK NTREAVDRWI 301 QENPNAAETV EAVFNVAAAA KVAKLAKAAK PGKAAVSGDF ADSYKKKLAL 351 SDSARQLYQN AKYREALDIH YEDLIRRKTD GSSKFINGRE IDAVTNDALI 401 QAKRTISAID KPKNFLNQKN RKQIKATIEA ANQQGKRAEF WFKYGVHSQV 451 KSYIESKGGI VKTGLGD* g730 / m730 93.0% identity in 344 aa overlap 10 20 30 40 50 60 g730.pep VKPLRRLTNLLAACAVAAVALIQPALAADLAQDPFITDNTQRQHYEPGGKYHLFGDPRGS I I I II II I I II II I II I I: II II II II II I II II II I I I : II II I II II I I II II II II I m730 VKPLRRLTNLLAACAVAAAALIQPALAADLAQDPFITDNAQRQHYEPGGKYHLFGDPRGS 10 20 30 40 50 60 70 80 90 100 110 120 g730.pep VSDRTGKINVIQDYTHQMGNLLIQQAAIQGNLGYTVRFSGHGHEEHAPFDNHAADSASEE I II II II I II I II I II II II II II II I: I :: II : II I II I II II I II II I I II II II I m730 VSDRTGKINVIQDYTHQMGNLLIQQANINGTIGYHTRFSGHGHEEHAPFDNHAADSASEE 70 80 90 100 110 120 130 140 150 160 170 180 g730. pep KGNVDDGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGARDEYTYHVNGTARSIKLNPT I I II M II II I II II II II I I II I II II II I II I II I II II I II II I II I II I II II I II m730 KGNVDEGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGARDEYTYHVNGTARSIKLNPT 130 140 150 160 170 180 190 200 210 220 230 240 g730.pep DTRSIRQRIFDNYNNLGSNFSDRADEANRKMFEHNAKLDRWGNSMEFVNGVAAGALNPFI I II M II II M I: II I I II II II I II II I I I II I I I I I I II I I II I: I I I I I II I II M m730 DTRSIRQRISDNYSNLGSNFSDRADEANRKMFEHNAKLDRWGNSMEFINGVAAGALNPFI 190 200 210 220 230 240 250 260 270 280 290 300 g730.pep SAGEALGIGDILYGTRYAIDKAAMRNIAPLPAEGKFAAIGGLGSAAGFEKNTREAVDRWI II II I II I I I II II II II I II II I I I II I II I II II I : II II I I: I II I II II II II I II m730 SAGEALGIGDILYGTRYAIDKAAMRNIAPLPAEGKFAVIGGLGSVAGFEKNTREAVDRWI 250 260 270 280 290 300 310 320 330 340 350 360 g730. pep QENPNAAETVEALVNVLPFAKVKNLTKAAKPGKAAVSGDFSKSYTCSFHGSTLVKTADGY II I I I II I I II M II III MMMMMMMMM II m 730 QENPNAAETVEAVFNVAAAAKVAKLAKAAKPGKAAVSGDFADSYKKKLALSDSARQLYQN 310 320 330 340 350 360 370 380 390 400 410 420 g730.pep KAIAHIQAGDRVLSKDEASGETGYKPVTARYGNPYQETVYIEVSDGIGNSQTLISNRIHP m730 AKYREALDIHYEDLIRRKTDGSSKFINGREIDAVTNDALIQAKRTIΞAIDKPKNFLNQKN 370 380 390 400 410 420 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2473>: a730.seq 1 GTGAAACCGC TGCGAAGACT CATCAAGCTC CTTGCCGCCT GTGCCGTAGC 51 GGCGGCCGCA CTCATACAGC CCGCCCTCGC GGCGGACTTG GCGCAAGACC 101 CGTTCATTAC CGATAACGCC CAACGGCAGC ACTACGAACC CGGAGGCAAA 151 TACCACCTCT TCGGCGACCC GCGCGGCAGC GTCTCCGACC GCACCGGTCA 201 AATCAACGTC ATCCAAGACT ATACCCACCG GATGGGCAAC CTGCTCATCC 251 AGCAGGCAAA CATCAACGGC ACAATCGGCT ACCACACCCG CTTTTCCGGA 301 CACGGATACG AAGAACACGC CCCCTTCGAC AACCACGCCG CCGACAGCGC 351 GAGCGAAGAA AAAGGCAACG TTGACGAAGG CTTTACCGTA TACCGGCTCA 401 ACTGGGAAGG ACACGAACAT CATCCCGCCG ATGCCTACGA CGGCCCGAAG 451 GGCGGCAATT ACCCCAAACC TACGGGTGCA CGCGACGAAT ACACCTATCA 501 CGTCAACGGC ACAGCACGCA GCATCAAACT CAATCCGACC GACACCCGCA 551 GCATCCGGCA ACGCATATCC GACAATTACA GCAACCTCGG CAGCAATTTC 601 TCCGACCGCG CCGATGAAGC CAACAGAAAA ATGTTCGAGC ACAATGCCAA 651 GCTCGACCGC TGGGGCAACA GCATGGAGTT TATCAACGGC GTCGCCGCCG 701 GCGCGCTCAA CCCCTTTATC AGCGCGGGCG AAGCCTTGGG CATAGGCGAC 751 ATACTGTACG GAACGCGCTA TGCCATAGAC AAAGCCGCAA TGCGCAACAT 801 CGCCCCCTTG CCCGCCGAGG GCAAATTCGC CGTCATCGGC GGCTTGGGCA 851 GCGTGGCGGG CTTTGAAAAA AATACGCGCG AAGCCGTTGA CCGGTGGATA 901 CAGGAAAACC CCAATGCCGC CGAAACCGTC GAAGCCCTGG TCAACGTCCT 951 GCCGTTTGCC AAAGTCAAAA ACCTGACAAA GGCGGCAAAA CCGGGGAAGG 1001 CTGCGGTTAG CGGGGATTTT TCTGCTGCAT ACAATACAAG AACAACTAGA 1051 AAAGTTACTA CAGAAACAGA GGGGTTAAAT AGAATCAGAC AGAACCAGAA 1101 AAATAGTAAT ATACATGAGA AAAATTATGG AAGAGATAAT CCTAATCATA 1151 TTAATGTTTT ATCTGGAAAT TCTATACAAC ATATACTGTA TGGAGATGAA 1201 GCAGGAGGTG GGCATCTTTT TCCTGGCAAA CCTGGTAAGA CAACATTCCC 1251 CCAACATTGG TCAGCCAGTA AAATAACTCA TGAAATTAGT GATATCGTTA 1301 CATCCCCAAA AACGCAATGG TATGCACAGA CTGGAACAGG CGGCAAATAT 1351 ATTGCTAAAG GAAGACCAGC TAGGTGGGTA TCATATGAAA CGAGAGATGG 1401 AATTCGTATC AGAACAGTTT ATGAACCTGC AACAGGAAAA GTGGTAACTG 1451 CATTCCCCGA TAGAACCTCT AATCCCAAAT ATAACCCTGT AAAATAA This corresponds to the amino acid sequence <SEQ ID 2474; ORF 730.a>: a730.pep 1 VKPLRRLIKL LAACAVAAAA LIQPALAADL AQDPFITDΝA QRQHYEPGGK 51 YHLFGDPRGS VSDRTGQIΝV IQDYTHRMGΝ LLIQQAΝIΝG TIGYHTRFSG 101 HGYEEHAPFD ΝHAADSASEE KGΝVDEGFTV YRLΝWEGHEH HPADAYDGPK 151 GGΝYPKPTGA RDEYTYHVΝG TARSIKLΝPT DTRSIRQRIS DΝYSΝLGSΝF 201 SDRADEAΝRK MFEHΝAKLDR WGΝSMEFIΝG VAAGALΝPFI SAGEALGIGD 251 ILYGTRYAID KAAMRΝIAPL PAEGKFAVIG GLGSVAGFEK ΝTREAVDRWI 301 QEΝPΝAAETV EALVΝVLPFA KVKΝLTKAAK PGKAAVSGDF SAAYΝTRTTR 351 KVTTETEGLΝ RIRQΝQKΝSΝ IHEKΝYGRDΝ PΝHIΝVLSGΝ SIQHILYGDE 401 AGGGHLFPGK PGKTTFPQHW SASKITHEIS DIVTSPKTQW YAQTGTGGKY 451 IAKGRPARWV SYETRDGIRI RTVYEPATGK VVTAFPDRTS ΝPKYΝPVK* a730 / m730 88.6% identity in 376 aa overlap 10 20 30 40 50 60 a730.pep VKPLRRLIKLLAACAVAAAALIQPALAADLAQDPFITDNAQRQHYEPGGKYHLFGDPRGS MIIIM : I II I II I I I I II II I II II I I I II II I I I I I I II II II I I II II I II I I I I m730 VKPLRRLTNLLAACAVAAAALIQPALAADLAQDPFITDNAQRQHYEPGGKYHLFGDPRGS 10 20 30 40 50 60 70 80 90 100 110 120 a730.pep VSDRTGQINVIQDYTHRMGNLLIQQANINGTIGYHTRFSGHGYEEHAPFDNHAADSASEE II II IM II II I II I I : I II II II I I II II I II II II I II I I: I II II II II I II II I II m730 VSDRTGKINVIQDYTHQMGNLLIQQANINGTIGYHTRFSGHGHEEHAPFDNHAADSASEE 70 80 90 100 110 120 130 140 150 160 170 180 a730.pep KGNVDEGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGARDEYTYHVNGTARSIKLNPT II II II II II II II I II I I II II I II I I I II II II II II II II II I II II I II II I II I I m730 KGNVDEGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGARDEYTYHVNGTARΞIKLNPT 130 140 150 160 170 180 190 200 210 220 230 240 a730. pep DTRSIRQRISDNYSNLGSNFSDRADEANRKMFEHNAKLDRWGNSMEFINGVAAGALNPFI II II II I II II II II II I I II II I II II I II II I II II II I II II I II II II I I II II II m730 DTRSIRQRISDNYSNLGSNFSDRADEANRKMFEHNAKLDRWGNSMEFINGVAAGALNPFI 190 200 210 220 230 240 250 260 270 280 290 300 a730.pep SAGEALGIGDILYGTRYAIDKAAMRNIAPLPAEGKFAVIGGLGSVAGFEKNTREAVDRWI II II II II II II II I II I II II II II II I II II II I II II II II II II I II II II II I I I m730 SAGEALGIGDILYGTRYAIDKAAMRNIAPLPAEGKFAVIGGLGSVAGFEKNTREAVDRWI 250 260 270 280 290 300 310 320 330 340 350 360 a730. pep QENPNAAETVEALVNVLPFAKVKNLTKAAKPGKAAVSGDFSAAYNTRTTRKVTTETEGLN I II II II II II I : I I II I : I : II I I I I II II II I I : : I : I : : : : m730 QENPNAAETVEAVFNVAAAAKVAKLAKAAKPGKAAVSGDFADSY KKKLALSDSAR 310 320 330 340 350 370 380 390 400 410 420 a730.pep RIRQNQKNSNIHEKNYGRDNPNHINVLSGNSIQHILYGDEAGGGHLFPGKPGKTTFPQHW :: I I I : : : I m730 QLYQNAKYREALDIHYEDLIRRKTDGSSKFINGREIDAVTNDALIQAKRTISAIDKPKNF 360 370 380 390 400 410 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2475>: g731. seq 1 gattttcgag cgttttcatG CGAGAACGGT TTGTCTGTGC GCGTCCGCAA 51 TTTGGACGGC GGCAAAATCG CGTTGCGGCT GGACGGCAGG CGTGCCGTCC 101 TCTCTTCCGA CGTTGCCGCA TCCGGCGAAC GCTATACCGC CGAACACGGT 151 TTGTTCGGAA ACGGAACCGA GTGGCACCAG AAAGGCGGCG AAGCCTTTTT 201 CGGCTTTACC GATGCCTACG GCAATTCGGT CGAAACTTCC TGCCGCGCCC 251 GTTAA This corresponds to the amino acid sequence <SEQ ID 2476; ORF 731.ng>: g731.pep 1 DFRAFSCEΝG LSVRVRΝLDG GKIALRLDGR RAVLSSDVAA SGERYTAEHG 51 LFGΝGTEWHQ KGGEAFFGFT DAYGΝSVETS CRAR* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2477>: m731. seq 1 ATGAATATCA GGTTTTTCGC GCTGACCGTA CCGGTTTTGT CTTTGGCGGC 51 CTGTGCCGTG CCGGAGGCGT ATGATGACGG CGGACGCGGG CATATGCCGC 101 CCGTTCAAAA CCAAGCCGGC ACGGACGATT TTCGGGCGTT TTCCTGCGAG 151 AACGGTTTGT CTGTGCGCGT CCGCCATTTG GACAGCGGCA AAGTCGCGTT 201 GCGGCTGGAC GGCAGGCGTG CCGTCCTCTC TTCCGACGTT GCCGCATCCG 251 GCGAACGCTA TACCGCCGAA CACGGTTTGT TCGGAAACGC AACCGAGTGG 301 CACCAGAAAG GCGGCGAAGC CTTTTTCGGC TTTACCGATG CCTACGGCAA 351 TTCGGTCGAA ACTTCCTGCC GCGCCCGTTA A This corresponds to the amino acid sequence <SEQ ID 2478; ORF 731>: m731.pep 1 MNIRFFALTV PVLSLAACAV PEAYDDGGRG HMPPVQNQAG TDDFRAFSCE 51 NGLSVRVRHL DSGKVALRLD GRRAVLSSDV AASGERYTAE HGLFGNATEW 101 HQKGGEAFFG FTDAYGNSVE TSCRAR* g73 l/m731 95.2% identity in 84 aa overlap 10 20 30 g731. pep DFRAFSCENGLSVRVRNLDGGKIALRLDGR II II I I I II II II I II : I I : I I : I I II I I I m731 LSLAACAVPEAYDDGGRGHMPPVQNQAGTDDFRAFSCENGLSVRVRHLDSGKVALRLDGR 20 30 40 50 60 70 40 50 60 70 80 g731. pep RAVLSSDVAASGERYTAEHGLFGNGTEWHQKGGEAFFGFTDAYGNSVETSCRARX II II II I II I II I II II I II II I I: I II I I II II II II I II II I II I II I II I II m731 RAVLSSDVAASGERYTAEHGLFGNATEWHQKGGEAFFGFTDAYGNSVETSCRARX 80 90 100 110 120 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2479>: a731. seq 1 ATGAATATCA GGTTTTTCGC GCTGACCGTA CCGGTTTTGT CTTTGGCGGC 51 CTGTGCCGTG CCGGAGGCGT ATGATGACGG CGGACGAGGG CATATGCCGC 101 CCGTTCAAAA CCAAGCCGGC ACGGCAGATT TTCGGGCATT TTCCTGCGAG 151 AACGGTTTGT CTGTGCACGT CCGCCGTTTG GACGGCGGCA GAATCGCGTT 201 GCGGTTGGAC GGCAGGCGTG CCGTCCTCTC TTCCGACGTT GCCGCATCCG 251 GCGAACGCTA TACCGCCGAA CACGGTTTGT TCGGAAACGG AACCGAGTGG 301 CATCAGAAAG GCGGCGAAGC CTTTTTCGGC TTTACCGATG CCTACGGCAA 351 TTCGGTCGAA ACCTCCTGCC GCGCCCGCTA A This corresponds to the amino acid sequence <SEQ ID 2480; ORF 731.a>: a731.pep 1 MNIRFFALTV PVLSLAACAV PEAYDDGGRG HMPPVQNQAG TADFRAFSCE 51 NGLSVHVRRL DGGRIALRLD GRRAVLSSDV AASGERYTAE HGLFGNGTEW 101 HQKGGEAFFG FTDAYGNSVE TSCRAR* a731/m731 94.4% identity in 126 aa overlap 10 20 30 40 50 60 a731. pep MNIRFFALTVPVLSLAACAVPEAYDDGGRGHMPPVQNQAGTADFRAFSCENGLΞVHVRRL II I II II II II II II II II I II II II II I I II II II II I I I II II II II II I I I : I I : I m731 MNIRFFALTVPVLSLAACAVPEAYDDGGRGHMPPVQNQAGTDDFRAFSCENGLSVRVRHL 10 20 30 40 50 60 70 80 90 100 , 110 120 a731. pep DGGRIALRLDGRRAVLSSDVAASGERYTAEHGLFGNGTEWHQKGGEAFFGFTDAYGNSVE I : I:: II I II I II II II II II II I II I II I II II I I: II II II II II II I II I II II II I m731 DSGKVALRLDGRRAVLSSDVAASGERYTAEHGLFGNATEWHQKGGEAFFGFTDAYGNSVE 70 80 90 100 110 120 a731.pep TSCRARX II I II II m731 TSCRARX The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2481>: g732. seq 1 ATGTCGAAAC CTGTTTTTAA GAAAATCGCA CTTTATACTT TGGGTGCAAT 51 CAGCGGCGTG GCCGTAAGTC TGGCGGTGCA GGGTTTTGCC GCCGagaagg 101 ACGGgcgGGA TAACGAagtC CTGCCGGTGC AATCCATCCG TACGATGGCG 151 GAGGTTTACG GTCAGATTAA GGCAAACTAC TATCATGACA AACCCGATGC 201 CGATTTGTTT GAAGGTGCGA TGAAGGGTAT GGTGGCCGGT TTGGATCCGC 251 ATTCCGAATA TATGGATAAA AAAGGTTATG CCGAGATAAA GGAGTCCACC 301 AGCGGCGAAT TTGGCGGCTT GGGGATGGAA ATCGGGCAGG AAGACGGTTT 351 TGTCAAAGTG GTTTCGCCGA TTGAGGACAC GCCTGCCGAA CGGGCGGAGG 401 TGAAAAGCGG CGATTTCATT GTGAAAATCG ATAATGTTTC GACGCGCGGT 451 ATGACGGTCA GCGAAGCGGT GAAAAAAATG CGGGGCAAGC CGGGTACGAA 501 GATTACTTTG ACGTTGTCGC GCAAAAATGC CGACAAGCCG ATAGTCGTCA 551 ACCTGACCCG TGCCATTATT AAAGTGAAAA GCGTCCGCCA TCACCTGATC 601 GAACCCGATT ACGGCTATAT CCGCGTGTCG CAGTTCCAAG AGCGGACGGT 651 CGAAAGCGTC AATACCGCCG CAAAAGAGCT GGTAAAGGAA AATAAAGGAA 701 AACCGCTCAA GGGGCTGGTG TTGGATTTGC GCGACGACCC CGGCGGGCTT 751 TTGACCGGCG CGGTCGGCGT GTCGGCGGCG TTTCTGCCGT CTGAAGCGGT 801 CGTCGTCAGC ACCAAGGGAC GCGACGGCAA AGACGGCATG GTACTGAAAG 851 CCGTTCCCGA GGATTATGTG TACGGTATGG GCGGCGACCC TTTGGCGGGT 901 ATTCCTGCCG AGTTGAAAAC GATTCCGATG ACGgtaTTGG TcaaTTCCGG 951 TTCggcttCC GCGTCGGAGA TTGtcgCCGG CGCATTGCAG GACCACAAAC 1001 GCGCGGTCAT CGTCGGTACG CAGAGCTTCG GTAAAGGTTC GGTTCAGACT 1051 TTGATTCCTT TGTCCAACGG CAGCGCGGTC AAGTTGACGA CCGCCCTGTA 1101 TTACACGCCG AACGACCGTT CCATTCAGGC ACAGGGGATT GTTCCCGATG 1151 TCgaaGTAAA AGATAAGGAA CGTACTTTTG AAAGCCGCGA GGCGGACCTG 1201 GTCGGACACA TCGGCAATCC CTTgggcGGC GAGGATGTGA ACAGTGAAAC 1251 CCttgcCGTA CCGCTTGAAA AAGATGCGGA TAAGCCCGCT GCAAAAGAAA 1301 AAGGTAAAAA GAAAAAGGAC GAGGATTTGT CTTCAAGGCG GATTCCGAAC 1351 CCTGCGAAAG ACGATCAGTT GCGTAAGGCT TTGGATTTGG TCAAGTCGCC 1401 CGAGCAGTGG CAGAAGTCTT TGGGGCTGGC GGCGAAAAAA CCGGTTTCAA 1451 ATAAAGATAA AAAAGATAAG AAGTAG This corresponds to the amino acid sequence <SEQ ID 2482; ORF 732>: g732.pep 1 MSKPVFKKIA LYTLGAISGV AVSLAVQGFA AEKDGRDNEV LPVQSIRTMA 51 EVYGQIKANY YHDKPDADLF EGAMKGMVAG LDPHSEYMDK KGYAEIKEST 101 SGEFGGLGME IGQEDGFVKV VSPIEDTPAE RAEVKSGDFI VKIDNVSTRG 151 MTVSEAVKKM RGKPGTKITL TLSRKNADKP IWNLTRAII KVKSVRHHLI 201 EPDYGYIRVS QFQERTVESV NTAAKELVKE NKGKPLKGLV LDLRDDPGGL 251 LTGAVGVSAA FLPSEAVWS TKGRDGKDGM VLKAVPEDYV YGMGGDPLAG 301 IPAELKTIPM TVLVNSGSAS ASEIVAGALQ DHKRAVIVGT QSFGKGSVQT 351 LIPLSNGSAV KLTTALYYTP NDRSIQAQGI VPDVEVKDKE RTFESREADL 401 VGHIGNPLGG EDVNSETLAV PLEKDADKPA AKEKGKKKKD EDLSSRRIPN 451 PAKDDQLRKA LDLVKSPEQW QKSLGLAAKK PVSNKDKKDK K* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2483>: m732. seq 1 ATGTCGAAAC CTGTTTTTAA GAAAATCGCA CTTTATACTT TGGGTGCAAT 51 CAGCGGCGTG GCCGTCAGTC TGGCGGTGCA GGGTTTTGCC GCCGAGAAGG 101 ACAGGCGGGA TAACGAAGTC CTGCCGGTGC AATCCATCCG CACAATGGCG 151 GAGGTTTACG GTCAAATCAA GGCAAACTAC TATCAGGACA AACCCGATGC 201 CGATTTGTTT GAAGGTGCGA TGAAGGGTAT GGTGGCCGGT TTGGATCCGC 251 ATTCCGAATA TATGGATAAA AAAGGTTATG CCGAGATAAA GGAGTCCACC 301 AGCGGCGAAT TTGGCGGCTT GGGGATGGAA ATCGGGCAGG AAGACGGATT 351 TGTCAAAGTG GTTTCGCCGA TTGAGGACAC GCCTGCGGAA CGGGCGGGGG 401 TGAAAAGCGG CGATTTCATT GTGAAAATCG ATAATGTTTC GACACGCGGC 451 ATGACGGTCA GCGAAGCGGT GAAGAAAATG CGGGGCAAGC CGGGTACGAA 501 GATTACTTTG ACGCTGTCGC GCAAAAATGC CGACAAGCCG ATAGTCGTCA 551 ACCTGACCCG TGCCATTATT AAAGTGAAAA GCGTCCGCCA TCACCTGATC 601 GAACCCGATT ACGGCTATAT CCGCGTGTCG CAGTTCCAAG AGCGGACGGT 651 CGAAAGCGTC AATACCGCCG CAAAAGAGCT GGTAAAGGAA AATAAAGGAA 701 AACCGCTCAA GGGGCTGGTG TTGGATTTGC GCGACGACCC CGGCGGGCTT 751 TTGACTGGCG CGGTCGGCGT GTCGGCGGCA TTTCTGCCGT CTGAAGCAGT 801 CGTCGTCAGC ACCAAGGGAC GCGACGGCAA AGACCGCATG GTACTGAAAG 851 CCATTCCTGA AGATTATGTG TACGGGATGG GCGGCGATTC GTTGGCGGGC 901 ATTCCTGCCG AGTTGAAAAC CATACCGATG ACGGTATTGG TCAATTCCGG 951 TTCGGCTTCC GCGTCGGAGA TTGTCGCAGG TGCATTGCAG GATCATAAAC 1001 GCGCGGTCAT CGTCGGTACG CAGAGCTTCG GCAAAGGTTC GGTTCAGACT 1051 TTGATTCCTT TGTCCAACGG CAGCGCGGTC AAGCTGACAA CGGCACTGTA 1101 TTATACGCCG AACGACCGTT CTATTCAGGC GCAGGGGATT GTTCCCGATG 1151 TCGAAGTAAA AGATAAGGAA CGCATTTTTG AAAGCCGCGA GGCGGATTTG 1201 GTCGGACACA TCGGCAATCC CTTGGGCGGC GAGGATGTGA ACGGTGAAAC 1251 CCTTGCCGTG CCGCTTGAAA AAGATGCGGA TAAGCCCGCT GTAAAAGAAA 1301 AAGGTAAAAA GAAAAAGGAC GAGGATTTGT CTTCAAGGCG GATTCCCAAC 1351 CCTGCCAAAG ACGACCAGTT GCGGAAAGCT TTGGATTTAG TCAAGTCGCC 1401 CGAGCAGTGG CAGAAGTCTT TGGGGCTGGC GGCGAAAAAG CCGGTTTCAA 1451 ATAAAGATAA GAAAGATAAA AAAGATAAGA AGTAG This corresponds to the amino acid sequence <SEQ ID 2484; ORF 732>: m732.pep 1 MSKPVFKKIA LYTLGAISGV AVSLAVQGFA AEKDRRDNEV LPVQSIRTMA 51 EVYGQIKANY YQDKPDADLF EGAMKGMVAG LDPHSEYMDK KGYAEIKEST 101 SGEFGGLGME IGQEDGFVKV VSPIEDTPAE RAGVKSGDFI VKIDNVSTRG 151 MTVSEAVKKM RGKPGTKITL TLSRKNADKP IWNLTRAII KVKSVRHHLI 201 EPDYGYIRVS QFQERTVESV NTAAKELVKE NKGKPLKGLV LDLRDDPGGL 251 LTGAVGVSAA FLPSEAVWS TKGRDGKDRM VLKAIPEDYV YGMGGDSLAG 301 IPAELKTIPM TVLVNSGSAS ASEIVAGALQ DHKRAVIVGT QSFGKGSVQT 351 LIPLSNGSAV KLTTALYYTP NDRSIQAQGI VPDVEVKDKE RIFESREADL 401 VGHIGNPLGG EDVNGETLAV PLEKDADKPA VKEKGKKKKD EDLSSRRIPN 451 PAKDDQLRKA LDLVKSPEQW QKSLGLAAKK PVSNKDKKDK KDKK* Computer analysis of the amino acid sequences gave the following results: Homology with a predicted ORF from N meningitidis menA with menB ORF 732 shows 98.2% identity over a 491 aa overlap with a predicted ORF (ORF732.a) from N gonorrhoeae: m732/g732 98.2% identity in 491 aa overlap 10 20 30 40 50 60 m732.pep MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDΝEVLPVQSIRTMAEVYGQIKAΝY II II I II I II II I II I II II II I II II II II I II II II II II II II II II II I II I I II g 732 MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDGRDΝEVLPVQSIRTMAEVYGQIKAΝY 10 20 30 40 50 60 70 80 90 100 110 120 m732. pep YQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSGEFGGLGMEIGQEDGFVKV I : II I I I II II I I I II II II II I II II II II I II II II I II II I II I II I II I I I II II I g 732 YHDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSGEFGGLGMEIGQEDGFVKV 70 80 90 100 110 120 130 140 150 160 170 180 m732.pep VSPIEDTPAERAGVKSGDFIVKIDΝVSTRGMTVSEAVKKMRGKPGTKITLTLSRKΝADKP I I I II II II I II II I II II I II I II I II II II II II II II II II I I II I I II I I II II I g732 VSPIEDTPAERAEVKSGDFIVKIDΝVSTRGMTVSEAVKKMRGKPGTKITLTLSRKΝADKP 130 140 150 160 170 180 190 200 210 220 230 240 m732.pep IWΝLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVESVΝTAAKELVKEΝKGKPLKGLV I I II I II II II II I I I II I I I II II II I I II I I I I I II I II II I II I II I II II I II I II g 32 IWΝLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVESVΝTAAKELVKEΝKGKPLKGLV 190 200 210 220 230 240 250 260 270 280 290 300 m732.pep LDLRDDPGGLLTGAVGVSAAFLPSEAVWSTKGRDGKDRMVLKAIPEDYVYGMGGDSLAG II I II II I I II II II I II I II II II I II II I II I II II II II M II II II I I I I I III g732 LDLRDDPGGLLTGAVGVSAAFLPSEAVWSTKGRDGKDGMVLKAVPEDYVYGMGGDPLAG 250 260 270 280 290 300 310 320 330 340 350 360 m732.pep IPAELKTIPMTVLVΝSGSASASEIVAGALQDHKRAVIVGTQSFGKGSVQTLIPLSΝGSAV I I I I I I I I M I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g732 IPAELKTIPMTVLVΝSGSASASEIVAGALQDHKRAVIVGTQSFGKGSVQTLIPLSΝGSAV 310 320 330 340 350 360 370 380 390 400 410 420 m732.pep KLTTALYYTPNDRSIQAQGIVPDVEVKDKERIFESREADLVGHIGNPLGGEDVNGETLAV I II I II II II I II I II II II I II II I II II I I I I II I II II I II II II I I II I : I II I I g 732 KLTTALYYTPNDRSIQAQGIVPDVEVKDKERTFESREADLVGHIGNPLGGEDVNSETLAV 370 380 390 400 410 420 430 440 450 460 470 480 m732.pep PLEKDADKPAVKEKGKKKKDEDLSSRRIPNPAKDDQLRKALDLVKSPEQWQKSLGLAAKK MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g 732 PLEKDADKPAAKEKGKKKKDEDLSSRRIPNPAKDDQLRKALDLVKSPEQWQKSLGLAAKK 430 440 450 460 470 480 490 m732. pep PVSNKDKKDKKDKKX II II II I II II g732 PVSNKDKKDKKX 490 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2485>: a732.seq 1 ATGTCGAAAC CTGTTTTTAA GAAAATCGCA CTTTATACTT TGGGTGCAAT 51 CAGCGGCGTG GCCGTCAGTC TGGCGGTGCA GGGTTTTGCC GCCGAGAAGG 101 ACAGGCGGGA TAACGAAGTC CTGCCGGTGC AATCCATCCG CACAATGGCG 151 GAGGTTTACG GTCAAATCAA GGCAAACTAC TATCAGGACA AACCCGATGC 201 CGATTTGTTT GAAGGTGCGA TGAAGGGTAT GGTGGCCGGT TTGGATCCGC 251 ATTCCGAATA TATGGATAAA AAAGGTTATG CCGAGATAAA GGAGTCCACC 301 AGCGGCGAAT TTGGCGGCTT GGGGATGGAA ATCGGGCAGG AAGACGGATT 351 TGTCAAAGTG GTTTCGCCGA TTGAGGACAC GCCTGCGGAA CGGGCGGGGG 401 TGAAAAGCGG CGATTTCATT GTGAAAATCG ATAATGTTTC GACACGCGGC 451 ATGACGGTCA GCGAAGCGGT GAAGAAAATG CGGGGCAAGC CGGGTACGAA 501 GATTACTTTG ACGCTGTCGC GCAAAAATGC CGACAAGCCG ATAGTCGTCA 551 ACCTGACCCG TGCCATTATT AAAGTGAAAA GCGTCCGCCA TCACCTGATC 601 GAACCCGATT ACGGCTATAT CCGCGTGTCG CAGTTCCAAG AGCGGACGGT 651 CGAAAGCGTC AATACCGCCG CAAAAGAGCT GGTAAAGGAA AATAAAGGAA 701 AACCGCTCAA GGGGCTGGTG TTGGATTTGC GCGACGACCC CGGCGGGCTT 751 TTGACTGGCG CGGTCGGCGT GTCGGCGGCA TTTCTGCCGT CTGAAGCAGT 801 CGTCGTCAGC ACCAAGGGAC GCGACGGCAA AGACCGCATG GTACTGAAAG 851 CCGTTCCTGA AGATTATGTG TACGGGATGG GCGGCGATTC GTTGGCGGGC 901 ATTCCTGCCG AGTTGAAAAC CATACCGATG ACGGTATTGG TCAATTCCGG 951 TTCGGCTTCC GCGTCGGAGA TTGTCGCAGG TGCATTGCAG GATCATAAAC 1001 GCGCGGTCAT CGTCGGTACG CAGAGCTTCG GCAAAGGTTC GGTTCAGACT 1051 TTGATTCCTT TGTCCAACGG CAGCGCGGTC AAGCTGACAA CGGCACTGTA 1101 TTATACGCCG AACGACCGTT CTATTCAGGC GCAGGGGATT GTTCCCGATG 1151 TCGAAGTAAA AGATAAGGAA CGCATTTTTG AAAGCCGCGA GGCGGATTTG 1201 GTCGGACACA TCGGCAATCC TTTGGGCGGC GAGGATGTGA ACAGTGAAAC 1251 CCTTGCCGTG CCGCTTGAAA AAGATGCGGA TAAGCCCGCT GTAAAAGAAA 1301 AAGGTAAAAA GAAAAAGGAC GAGGATTTGT CTTCAAGGCG GATTCCCAAC 1351 CCTGCCAAAG ACGACCAGTT GCGGAAAGCT TTGGATTTAG TCAAGTCGCC 1401 CGAGCAGTGG CAGAAGTCTT TGGGGCTGGC GGCGAAAAAG CCGGTTTCAA 1451 ATAAAGATAA GAAAGATAAA AAAGATAAGA AGTAG This corresponds to the amino acid sequence <SEQ ID 2486; ORF 732. a>: a732.pep 1 MSKPVFKKIA LYTLGAISGV AVSLAVQGFA AEKDRRDNEV LPVQSIRTMA 51 EVYGQIKANY YQDKPDADLF EGAMKGMVAG LDPHSEYMDK KGYAEIKEST 101 SGEFGGLGME IGQEDGFVKV VSPIEDTPAE RAGVKSGDFI VKIDNVSTRG 151 MTVSEAVKKM RGKPGTKITL TLSRKNADKP IWNLTRAII KVKSVRHHLI 201 EPDYGYIRVS QFQERTVESV NTAAKELVKE NKGKPLKGLV LDLRDDPGGL 251 LTGAVGVSAA FLPSEAVWS TKGRDGKDRM VLKAVPEDYV YGMGGDSLAG 301 IPAELKTIPM TVLVNSGSAS ASEIVAGALQ DHKRAVIVGT QSFGKGSVQT 351 LIPLSNGSAV KLTTALYYTP NDRSIQAQGI VPDVEVKDKE RIFESREADL 401 VGHIGNPLGG EDVNSETLAV PLEKDADKPA VKEKGKKKKD EDLSSRRIPN 451 PAKDDQLRKA LDLVKSPEQW QKSLGLAAKK PVSNKDKKDK KDKK* a732/m732 99.6% identity in 494 aa overlap 10 20 30 40 50 60 a732. pep MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQSIRTMAEVYGQIKANY II I II II II II II II II II I I II II I II II I II II I II I I II I II II I II II I I II II II m732 MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQSIRTMAEVYGQIKANY 10 20 30 40 50 60 70 80 90 100 110 120 a732. pep YQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSGEFGGLGMEIGQEDGFVKV II II II II II II II I II I II I II II I II I II II II II II I I I I II II I I II I I II II II I m732 YQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSGEFGGLGMEIGQEDGFVKV 70 80 90 100 110 120 130 140 150 160 170 180 a732.pep VSPIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKMRGKPGTKITLTLSRKNADKP II II II I II I II II II II II II II II II I II I II I I I I I II II I II II I I I II I II I II I m732 VSPIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKMRGKPGTKITLTLSRKNADKP 130 140 150 160 170 180 190 200 210 220 230 240 a732.pep IWNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVESVNTAAKELVKENKGKPLKGLV MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM m732 IWNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVESVNTAAKELVKENKGKPLKGLV 190 200 210 220 230 240 250 260 270 280 290 300 a732.pep LDLRDDPGGLLTGAVGVSAAFLPSEAVWSTKGRDGKDRMVLKAVPEDYVYGMGGDSLAG II II II I II I II II I II I II II II II II II I II II I II II II II M II I II I I I II II II m732 LDLRDDPGGLLTGAVGVSAAFLPSEAVWSTKGRDGKDRMVLKAIPEDYVYGMGGDSLAG 250 260 270 280 290 300 310 320 330 340 350 360 a732. pep IPAELKTIPMTVLVNSGSASASEIVAGALQDHKRAVIVGTQSFGKGSVQTLIPLSNGSAV II I II II II II II I I II I I II II II I II I II II I II II II II I II I I I II II II II I I I I m 732 IPAELKTIPMTVLVNSGSASASEIVAGALQDHKRAVIVGTQSFGKGSVQTLIPLSNGSAV 310 320 330 340 350 360 370 380 390 400 410 420 a732.pep KLTTALYYTPNDRSIQAQGIVPDVEVKDKERIFESREADLVGHIGNPLGGEDVNSETLAV II II II II I II I II I II I I II II II I II I I I II I II II II I II II I I I I II I II : II II I m732 KLTTALYYTPNDRSIQAQGIVPDVEVKDKERIFESREADLVGHIGNPLGGEDVNGETLAV 370 380 390 400 410 420 430 440 450 460 470 480 a732. pep PLEKDADKPAVKEKGKKKKDEDLSSRRIPNPAKDDQLRKALDLVKSPEQWQKSLGLAAKK II II I II II II II II II I I II II II I II I II I I I II II II I II II II I II II I II I II I I m732 PLEKDADKPAVKEKGKKKKDEDLSSRRIPNPAKDDQLRKALDLVKSPEQWQKSLGLAAKK 430 440 450 460 470 480 490 a732. pep PVSNKDKKDKKDKKX II II I II II II II II m732 PVSNKDKKDKKDKKX 490 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2487>: g733. seq 1 ATGATGAATC CGAAAACCTT GGGCCGTTTG TCGCTGTGTG CGGCGGTCTT 51 GGCTCTGACC GCCTGCGCCG GCGGCGGGCA TAAAAACCTG TATTATTACG 101 GCGGTTATCC CGATACCGTC TATGAAGGTT TGAAAAACGa cgACACTTCG 151 TTGGGCAAGC AGACCGAAAA GATGGAAAAA TACTTTGCGG AAGCCGCCAA 201 CAAAAAAATG AATGCCGCCC CGGGTGCGCA CGCCCATTTG GGACTGCTGC 251 TTTCCCGTTC GGGAGACAAA GAGGGCGCGT TCCGCCAATT TGAAGAAGAG 301 AAAAGGCTGT TTCCCGAATC GGGCGTATTT ATGGACTTCC TGATGAAAAC 351 CGGtaaAGGA GGCAAGCGAT GA This corresponds to the amino acid sequence <SEQ ID 2488; ORF 733>: g733.pep 1 MMNPKTLGRL SLCAAVLALT ACAGGGHKNL YYYGGYPDTV YEGLKNDDTS 51 LGKQTEKMEK YFAEAANKKM NAAPGAHAHL GLLLSRSGDK EGAFRQFEEE 101 KRLFPESGVF MDFLMKTGKG GKR* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2489>: π»733. seq 1 ATGATGAATC CGAAAACCTT GAGCCGTTTG TCGCTGTGTG CGGCGGTCTT 51 GGCTCTGACC GCCTGCGGCG GCAACGGGCA AAAATCCCTG TATTATTACG 101 GCGGCTATCC CGATACCGTC TATGAAGGTT TGAAAAACGA CGACACTTCG 151 TTGGGCAAGC AGACCGAAAA GATGGAAAAA TACTTTGTGG AAGCCGGCAA 201 CAAAAAAATG AATGCCGCCC CGGGTGCGCA CGCCCATCTG GGACTGCTGC 251 TTTCCCGTTC GGGAGACAAA GAGGGCGCGT TCCGCCAGTT TGAAGAAGAG 301 AAAAGGCTGT TTCCCGAATC GGGCGTATTT ATGGACTTCC TGATGAAAAC 351 CGGTAAAGGA GGCAAGCGAT GA This corresponds to the amino acid sequence <SEQ ID 2490; ORF 733>: m733.pep 1 MMΝPKTLSRL SLCAAVLALT ACGGΝGQKSL YYYGGYPDTV YEGLKNDDTS 51 LGKQTEKMEK YFVEAGNKKM NAAPGAHAHL GLLLSRSGDK EGAFRQFEEE 101 KRLFPESGVF MDFLMKTGKG GKR* Computer analysis of the amino acid sequences gave the following results: Homology with a predicted ORF from N meningitidis menA with menB ORF 733 shows 94.3% identity over a 123 aa overlap with a predicted ORF (ORF733.a) from N gonorrhoeae: m733/g733 10 20 30 40 50 60 m733. pep MMΝPKTLSRLSLCAAVLALTACGGΝGQKSLYYYGGYPDTVYEGLKΝDDTSLGKQTEKMEK I II I II M II II II II II II II : M M M II I I II II I II II I II II II II I II II II I I g733 MMΝPKTLGRLSLCAAVLALTACAGGGHKΝLYYYGGYPDTVYEGLKΝDDTSLGKQTEKMEK 10 20 30 40 50 60 70 80 90 100 110 120 m 33.pep YFVEAGΝKKMΝAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRLFPESGVFMDFLMKTGKG I M I M I II II I I I I II II I II I II II II I II I II I II II II II II I II II II II I II II g733 YFAEAAΝKKMΝAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRLFPESGVFMDFLMKTGKG 70 80 90 100 110 120 m733.pep GKRX II II g733 GKRX The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2491>: a733. seq 1 ATGATGAATC CGAAAACCTT GAGCCGTTTG TCGCTGTGTG CGGCGGTCTT 51 GGCTCTGACC GCCTGCGGCG GCAACGGGCA AAAATCCCTG TATTATTACG 101 GCGGCTATCC CGATACCGTC TATGAAGGTT TGAAAAACGA CGACACTTCG 151 TTGGGCAAGC AGACCGAAAA GATGGAAAAA TACTTTGTGG AAGCCGGCAA 201 CAAAAAAATG AATGCCGCCC CGGGTGCGCA CGCCCATCTG GGACTGCTGC 251 TTTCCCGTTC GGGAGACAAA GAGGGCGCGT TCCGCCAGTT TGAAGAAGAG 301 AAAAGGCTGT TTCCCGAATC GGGCGTATTT ATGGACTTCC TGATGAAAAC 351 CGGTAAAGGA GGCAAGCGAT GA This corresponds to the amino acid sequence <SEQ ID 2492; ORF 733. a>: a733.pep 1 MMNPKTLSRL SLCAAVLALT ACGGNGQKSL YYYGGYPDTV YEGLKNDDTS 51 LGKQTEKMEK YFVEAGNKKM NAAPGAHAHL GLLLSRSGDK EGAFRQFEEE 101 KRLFPESGVF MDFLMKTGKG GKR* a733/m733 100.0% identity in 123 aa overlap 10 20 30 40 50 60 a733.pep MMNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLKNDDTSLGKQTEKMEK I II I II II II II II II I II I II II II II I I II II II II I I II II II I I II I I I II II II I 733 MMNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLKNDDTSLGKQTEKMEK 10 20 30 40 50 60 70 80 90 100 110 120 a733. pep YFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRLFPESGVFMDFLMKTGKG I II II II I I II I II II II II II I I II II I I II II I II II II II I II I II I I II I II II II m733 YFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRLFPESGVFMDFLMKTGKG 70 80 90 100 110 120 a733.pep GKRX MM m733 GKRX The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2493>: g734.seq 1 ATGATGAAAA AGATACTGGC AGTATCGGCA CTATGCCTGA TGACTGCGGC 51 GGCACAGGCT GCCGATACTT ACGGCTATCT CGCCGTTTGG CAGAATCCGC 101 AGGATGCAAA CGATGTTTTG CAGGTTAAAA CCACAAAAGA AGATTCGGCG 151 AAAAGCGAAG CGTTTGCCGA GTTGGAAGCC TTTTGCAAAG GTCAGGACAC 201 GCTTGCGGGC ATTGCCGAAG ACGAGCCGAC CGGATGCCGG TCGGTCGTGT 251 CGCTGAACAA TACCTGTGTC TCGCTGGCAT ACCCGAAAGC CTTGGGCGCG 301 ATGCGCGTTG AAAACGCCGT CGTGATTACT TCTCCGCGTT TTACGAGCGT 351 TCATCAGGTC GCACTCAACC AGTGCATAAA AAAATACGGC GCACAGGGAC 401 AATGCGGCTT GGAAACAGTG TATTGCACGT CATCTTCTTA TTACGGCGGG 451 GCTGTTCGCT CCTTAATCCA ACACCTGAAA TAA This corresponds to the amino acid sequence <SEQ ID 2494; ORF 734.ng>: g734.pep 1 MMKKILAVSA LCLMTAAAQA ADTYGYLAVW QΝPQDAΝDVL QVKTTKEDSA 51 KSEAFAELEA FCKGQDTLAG IAEDEPTGCR SWSLΝΝTCV SLAYPKALGA 101 MRVEΝAWIT SPRFTSVHQV ALΝQCIKKYG AQGQCGLETV YCTSSSYYGG 151 AVRSLIQHLK * The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2495>: m734.seq (partial) 1 TCGGGCATTG CTGAAGACGA GCCGACCGGA TGCCGGTCGG TCGTGTCGCT 51 GAACAATACC TGTGTCGCGC TGGCATACCC GAAAGCCTTG GGCGCGCTGC 101 GTGTCGACAA CGCCGTCGTG ATTACTTCTC CGCGTTTTAC GAGCGTTCAT 151 CAGGTCGCAC TCAACCAGTG CATCAAAAAA TACGGCGTAC AGGGACAATG 201 CGGCTTGGAA ACAGTGTATT GCACATCTTC TTCTTATTAC GGCGGAACTG 251 TGCGCTCTTT GATTCAAAAT CTCAAATAA This corresponds to the amino acid sequence <SEQ ID 2496; ORF 734>: m734.pep (partial) 1 SGIAEDEPTG CRSWSLΝΝT CVALAYPKAL GALRVDΝAW ITSPRFTSVH 51 QVALΝQCIKK YGVQGQCGLE TVYCTSSSYY GGTVRSLIQΝ LK* m734/g734 92.4% identity in 92 aa overlap 10 20 30 m734 . pep SGIAEDEPTGCRSWSLΝΝTCVALAYPKAL M I I I I I I I I I I I I I I I I I I I I : I I I I I I I g734 VLQVKTTKEDSAKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSWSLΝΝTCVSLAYPKAL 40 50 60 70 80 90 40 50 60 70 80 90 m734 . pep GALRVDNAVVITSPRFTSVHQVALNQCIKKYGVQGQCGLETVYCTSSSYYGGTVRSLIQN I I : I I : I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I : I I I I I I : g734 GAMRVENAWITSPRFTSVHQVALNQCIKKYGAQGQCGLETVYCTSSSYYGGAVRSLIQH 100 110 120 130 140 150 m73 . pep LKX I I I g734 LKX 160 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2497>: a734.seq 1 ATGATGAAAA AGATACTGGC CGTATCGGCA CTATGCCTGA TGACTGCGGC 51 GGCACGGGCT GCCGATACTT ACGGCTATCT CGCCGTTTGG CAGAATCCGC 101 AGAATGCAAA CGATGTTTTG CAGGTTAAAA CCACAAAAGA AGATTCGACG 151 AAAAGCGAAG CGTTTGCCGA GTTGGAAGCT TTCTGCAAAG GTCAGGACAC 201 GCTTGCGGGC ATTGCCGAAG ACGAGCCGAC CGGATGCCGG TCGGTCGTGT 251 CGCTGAACAA TACCTGTGTC GCGCTGGCAT ACCCGAAAGC CTTGGGCGCG 301 ATGCGCGTTG AAAACGCCGT TGTGATTACT TCTCCGCGTT TTACGAGCGT 351 TTATCAGGTC GCACTCAACC AGTGCATCAA AAAATACGGC GCACAGGGAC 401 AATGCGGCTT GGAAACAGTG TATTGCACGT CTTCTTCTTA TTACGGGGGA 451 ACTGTGCGCT CTTTGATTCA AAATCTCAAA TAA This corresponds to the amino acid sequence <SEQ ID 2498; ORF 734.a>: a734.pep 1 MMKKILAVSA LCLMTAAARA ADTYGYLAVW QΝPQΝAΝDVL QVKTTKEDST 51 KSEAFAELEA FCKGQDTLAG IAEDEPTGCR SWSLΝΝTCV ALAYPKALGA 101 MRVEΝAWIT SPRFTSVYQV ALΝQCIKKYG AQGQCGLETV YCTSSSYYGG 151 TVRSLIQΝLK * a734/g734 95.6% identity in 160 aa overlap 10 20 30 40 50 60 a734.pep MMKKILAVSALCLMTAAARAADTYGYLAVWQΝPQΝAΝDVLQVKTTKEDSTKSEAFAELEA II I II II II I II II I II I : I I II II I II I II II I : II I I II II II II I I : I II II II I I I g734 MMKKILAVSALCLMTAAAQAADTYGYLAVWQΝPQDAΝDVLQVKTTKEDSAKSEAFAELEA 10 20 30 40 50 60 70 80 90 100 110 120 a734. pep FCKGQDTLAGIAEDEPTGCRSVVSLΝΝTCVALAYPKALGAMRVEΝAWITSPRFTSVYQV II I I II I II II II II I II II I II II II II I : I I I I II I II II II I I I II II II I I II : I I g734 FCKGQDTLAGIAEDEPTGCRSWSLΝΝTCVSLAYPKALGAMRVEΝAVVITSPRFTSVHQV 70 80 90 100 110 120 130 140 150 160 a73 .pep ALΝQCIKKYGAQGQCGLETVYCTSSSYYGGTVRSLIQΝLKX I II II II II I II II II II II II II II I I I I : II I II I : I II g734 ALΝQCIKKYGAQGQCGLETVYCTSSSYYGGAVRSLIQHLKX 130 140 150 160 g735. seq not found yet g735.pep not found yet The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2499>: m735. seq 1 ATGAATCTCG TGAAACTGCT GGCGAATAAC TGGCAACCGA TTGCCATTAT 51 CGCGCTTGTC GGCACGGGCT TGGCTGTGTC GCACCATCAA GGCTACAAGT 101 CGGCATTTGC GAAGCAGCAG GCGGTCATCG ACAAGATGGA GCGCGACAAG 151 GCGCAAGCCC TGCTGTTGTC GGCTCAAAAC TATGCGCGCG AACTGGAACT 201 GGCACGCGCG GAAGCTAAAA AATATGAAGT CAAGGCGCAC GCTGTCGGCA 251 TGGCTTTGGC GAAAAAACAG GCGGAAGTCA GCCGTCTGAA AACGGAAAAT 301 AAAAAGGAAA TCGAAAATGT CCTTACTCAA GACCGTAAAA ATGCAAGCGG 351 CGGTTGCATT GACGGCTTTG GCTCTCACGG CCTGCAGCTC TACAACCGCG 401 CCCTCGGCTA CGGAAATTAA This corresponds to the amino acid sequence <SEQ ID 2500; ORF 735>: m735.pep 1 MNLVKLLANN WQPIAIIALV GTGLAVSHHQ GYKSAFAKQQ AVIDKMERDK 51 AQALLLSAQN YARELELARA EAKKYEVKAH AVGMALAKKQ AEVSRLKTEN 101 KKEIENVLTQ DRKNASGGCI DGFGSHGLQL YNRALGYGN* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2501>: a735.seq 1 ATGAATCTCG TGAAACTGCT GGCGAATAAC TGGCAACCGA TTGCCATCAT 51 CGCGCTTGTC GGCACGGGTT TGGCGGTGTC GCACCATCAA GGCTACAAGT 101 CGGCTTTTGC GAAGCAGCAG GCGGTCATTG AGAAAATGAA GCGCGACAAG 151 GCGCAAGCCC TGCTGTTGTC GGCTCAAAAC TACGCCCGCG AACTGGAACA 201 GGCGCGTGCG GAAGCTAAAA AATATGAAGT CAAGGCGCAC GCCGTCGGCA 251 TGGCTTTGGC GAAAAAACAG GCGGAAGTCA GCCGTCTGAA AACGGAAAAT 301 AAAAAGGAAA TCGAAAATGT CCTTACTCAA GACCGTAAAA ATGCAGGCGG 351 CGGTTGTATT GACGGCTTTG GCCATCACGG CTTGCAGCTC TACAAGCGCG 401 CCCTCGGCTA CGGAAATTAA This corresponds to the amino acid sequence <SEQ ID 2502; ORF 735.a>: a735.pep 1 MΝLVKLLAΝΝ WQPIAIIALV GTGLAVSHHQ GYKSAFAKQQ AVIEKMKRDK 51 AQALLLSAQΝ YARELEQARA EAKKYEVKAH AVGMALAKKQ AEVSRLKTEN 101 KKEIENVLTQ DRKNAGGGCI DGFGHHGLQL YKRALGYGN* a735/m735 95.7% identity in 139 aa overlap 10 20 30 40 50 60 a735. pep MNLVKLLANNWQPIAIIALVGTGLAVSHHQGYKSAFAKQQAVIEKMKRDKAQALLLSAQN II II I II II I II II I I II I I II I II I I II I II II I I II II II I : I I: II I II I I II II I I m735 MNLVKLLANNWQPIAIIALVGTGLAVSHHQGYKSAFAKQQAVIDKMERDKAQALLLSAQN 10 20 30 40 50 60 70 80 90 100 110 120 a735.pep YARELEQARAEAKKYEVKAHAVGMALAKKQAEVSRLKTENKKEIENVLTQDRKNAGGGCI MMM II II II II II I I II II II I II I II II II II II II II I II I II II I I I I : I I II m735 YARELELARAEAKKYEVKAHAVGMALAKKQAEVSRLKTENKKEIENVLTQDRKNASGGCI 70 80 90 100 110 120 130 140 a735.pep DGFGHHGLQLYKRALGYGNX II II I II I II : II I II I I I m735 DGFGSHGLQLYNRALGYGNX 130 140 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2503>: g736.seq 1 ATGAATTTTA TCCGTTCCGT CGGGGCGAAA ACCCTCGGCC TTATTCAATC 51 CTTCGGCAGT ATCACGCTGT TTCTGCTGAA CATTTTGGCG AAATCCGGCA 101 CGGCTTTCGC CCGTCCGCGC CTGAGCGTGC GCCAAGTGTA TTTTGCCGGC 151 GTGCTGTCGG TGCTGATTGT TGCCGTTTCG GGGCTGTTCG TCGGTATGGT 201 TTTGGGTTTG CAGGGCTATA CGCAGTTGTC GAAATTCAAA TCCGCCGATA 251 TTTTGGGCTA TATGGTCGCG GCTTCTCTGT TGCGCGAACT GGGTCCCGTG 301 TTGGCGGCGA TTCTGTTTGC CAGCAGCGCG GGCGGTGCGA TGACCAGCGA 351 AATCGGTTTG ATGAAAACGA CCGGACAGCT CGAAGCGATG AACGTGATGG 401 CGGTCAACCC CGTCGCCCGC GTGGTTGCCC CGCGTTTTTG GGCGGGCGTG 451 TTTTCTATGC CGCTTTTGGC TTCGATTTTC AACGTCGCGG GCATTTTCGG 501 CGCGTATTTG GTCGGCGTGA GCTGGCTGGG TTTGGACAGC GGTATTTTCT 551 GGCCGCAGAT GCAGAACAAC ATTACGATAC ATTACGATGT AATCAACGGT 601 TTGATCAAAT CCGCCGCGTT CGGCGTGGCG GTAACGCTGA TTGCCGTGCA 651 TCAGGGCTTC CACTGCATCC CGACTTCGGA AGGCATTTTG CGCGCCAGCA 701 CGCGCACGGT GGTTTCGTCC GCCCTGACGA TTTTGGCGGT CGATTTTATA 751 TTGACCGCGT GGATGTTTAC AGATTGA This corresponds to the amino acid sequence <SEQ ID 2504; ORF 736>: g736.pep 1 MNFIRSVGAK TLGLIQSFGS ITLFLLNILA KSGTAFARPR LSVRQVYFAG 51 VLSVLIVAVS GLFVGMVLGL QGYTQLSKFK SADILGYMVA ASLLRELGPV 101 LAAILFASSA GGAMTSEIGL MKTTGQLEAM NVMAVNPVAR WAPRFWAGV 151 FSMPLLASIF NVAGIFGAYL VGVSWLGLDS GIFWPQMQNN ITIHYDVING 201 LIKSAAFGVA VTLIAVHQGF HCIPTSEGIL RASTRTWSS ALTILAVDFI 251 LTAWMFTD* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2505>: m736. seq 1 ATGAATTTTA TCCGTTCCGT CGGGGCGAAA ACCCTCGGCC TTATTCAATC 51 CTTCGGCAGT ATCACGCTGT TTCTGCTGAA CATTTTGGCG AAATCCGGCA 101 CGGCTTTCGC CCGTCCGCGC CTGAGCGTGC GCCAAGTGTA TTTTGCCGGC 151 GTGCTGTCGG TGCTGATTGT TGCCGTTTCG GGGCTGTTCG TCGGTATGGT 201 TTTGGGTTTG CAGGGCTATA CGCAGTTGTC GAAATTCAAA TCCGCCGATA 251 TTTTGGGCTA TATGGTCGCG GCTTCTCTGT TGCGCGAACT GGGTCCCGTG 301 TTGGCGGCGA TTCTGTTTGC CAGCAGCGCG GGCGGTGCGA TGACCAGCGA 351 AATCGGTTTG ATGAAAACGA CCGGACAGCT CGAAGCGATG AACGTGATGG 401 CGGTCAACCC CGTCGCCCGC GTGGTTGCCC CGCGTTTTTG GGCGGGCGTG 451 TTTTCTATGC CGCTTTTGGC TTCGATTTTC AACGTCGCGG GCATTTTCGG 501 CGCGTATTTG GTCGGCGTGA GCTGGCTGGG TTTGGACAGC GGTATTTTCT 551 GGCCGCAGAT GCAGAACAAC ATTACGATAC ATTACGATGT AATCAACGGT 601 TTGATCAAAT CCGCCGCGTT CGGCGTGGCG GTAACGCTGA TTGCCGTGCA 651 TCAGGGCTTC CACTGCATCC CGACTTCGGA AGGCATTTTG CGCGCCAGCA 701 CGCGCACGGT GGTTTCGTCC GCCCTGACGA TTTTGGCGGT CGATTTTATA 751 TTGACCGCGT GGATGTTTAC AGATTGA This corresponds to the amino acid sequence <SEQ ID 2506; ORF 736>: m736.pep 1 MNFIRSVGAK TLGLIQSLGS ITLFLLNILA KSGTAFVRPR LSVRQVYFAG 51 VLSVLIVAVS GLFVGMVLGL QGYTQLSKFK SADILGYMVA ASLLRELGPV 101 LAAILFASSA GGAMTSEIGL MKTTEQLEAM NVMAVNPVAR WAPRFWAGV 151 FSMPLLASIF NVAGIFGAYL VGVTWLGLDS GIFWSQMQNN ITIHYDVING 201 LIKSAAFGVA VTLIAVHQGF HCVPTSEGIL RASTRTWSS ALTILAVDFI 251 LTAWMFTD* Computer analysis of the amino acid sequences gave the following results: Homology with a predicted ORF from N meningitidis menA with menB ORF 736 shows 97.7% identity over a 258 aa overlap with a predicted ORF (ORF736.ng) from N. gonorrhoeae: m736/g736 10 20 30 40 50 60 m736.pep MΝFIRSVGAKTLGLIQSLGSITLFLLΝILAKSGTAFVRPRLSVRQVYFAGVLSVLIVAVS II II I II II I I I I II I M II II II II II II I II II M II I II II II I II II II II II II I g736 MΝFIRSVGAKTLGLIQSFGSITLFLLΝILAKSGTAFARPRLSVRQVYFAGVLSVLIVAVS 10 20 30 40 50 60 70 80 90 100 110 120 m736.pep GLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLAAILFASSAGGAMTSEIGL II II I I I II I II II I II II I II I I II I I II I I II I II I II I II II II II I I II I I II II I g736 GLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLAAILFASSAGGAMTSEIGL 70 80 90 100 110 120 130 140 150 160 170 180 m736. pep MKTTEQLEAMΝVMAVΝPVARWAPRFWAGVFSMPLLASIFΝVAGIFGAYLVGVTWLGLDS M II II II I I II II II I I I II I II II II II II I II II II I II I II I II II I I: I II II I g736 MKTTGQLEAMΝVMAVΝPVARVVAPRFWAGVFSMPLLASIFΝVAGIFGAYLVGVSWLGLDS 130 140 150 160 170 180 190 200 210 220 230 240 m736.pep GIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQGFHCVPTSEGILRASTRTWSS llll II II II II II II II II II I II I I I II I II I II II II I: I I I II I II I I I II I II I g736 GIFWPQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQGFHCIPTSEGILRASTRTWSS 190 200 210 220 230 240 250 259 m736.pep ALTILAVDFILTAWMFTDX II I II II II II II II II II g736 ALTILAVDFILTAWMFTDX 250 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2507>: a736.seq 1 ATGAATTTTA TCCGTTCCGT CGGGGCGAAA ACCCTCGGCC TTATTCAATC 51 TCTCGGCAGT ATCACGCTGT TTCTGCTGAA TATTCTGGCG AAATCCGGTA 101 CGGCTTTCGT CCGTCCGCGC CTGAGCGTGC GCCAAGTGTA TTTTGCCGGC 151 GTGCTGTCGG TGTTGATTGT TGCCGTTTCA GGGCTGTTTG TCGGCATGGT 201 CTTGGGTTTG CAGGGCTATA CGCAGTTGTC GAAATTCAAA TCCGCCGATA 251 TTTTGGGCTA TATGGTCGCG GCTTCGCTGT TGCGCGAACT GGGTCCGGTG 301 TTGGCGGCGA TTCTGTTTGC CAGCAGCGCG GGCGGTGCGA TGACCAGCGA 351 AATCGGTTTG ATGAAAACGA CCGAACAGCT CGAAGCGATG AACGTGATGG 401 CGGTAAACCC CGTCGCCCGA GTGGTTGCGC CGCGCTTTTG GGCGGGCGTG 451 TTTTCCATGC CGCTTTTGGC TTCGATTTTC AACGTGGCGG GTATTTTCGG 501 CGCGTATTTG GTCGGTGTAA CCTGGCTGGG CTTGGACAGC GGTATTTTCT 551 GGTCGCAAAT GCAGAACAAC ATCACGATAC ATTACGATGT AATCAACGGT 601 CTGATCAAAT CCGCCGCGTT CGGCGTGGCG GTAACGCTGA TTGCCGTGCA 651 TCAGGGCTTC CACTGCGTCC CGACCTCGGA AGGCATTTTG CGCGCCAGCA 701 CGCGCACGGT GGTTTCGTCC GCCCTGACGA TTTTGGCGGT CGATTTTATA 751 TTGACCGCGT GGATGTTTAC AGATTGA This corresponds to the amino acid sequence <SEQ ID 2508; ORF 736. a>: a736.pep 1 MNFIRSVGAK TLGLIQSLGS ITLFLLNILA KSGTAFVRPR LSVRQVYFAG 51 VLSVLIVAVS GLFVGMVLGL QGYTQLSKFK SADILGYMVA ASLLRELGPV 101 LAAILFASSA GGAMTSEIGL MKTTEQLEAM NVMAVNPVAR WAPRFWAGV 151 FSMPLLASIF NVAGIFGAYL VGVTWLGLDS GIFWSQMQNN ITIHYDVING 201 LIKSAAFGVA VTLIAVHQGF HCVPTSEGIL RASTRTWSS ALTILAVDFI 251 LTAWMFTD* a736/m736 100.0% identity in 258 aa overlap 10 20 30 40 50 60 a736. pep MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVRQVYFAGVLSVLIVAVS II II I II II I I II II II I II II I II II II I II II II II II I II II II II I I I II I II II I m736 MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVRQVYFAGVLSVLIVAVS 10 20 30 40 50 60 70 80 90 100 110 120 a736.pep GLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLAAILFASSAGGAMTSEIGL II II I I II II I II II I II II II II I II II I I I II I I II II I II I I II II I II I II I I I II m736 GLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLAAILFASSAGGAMTSEIGL 70 80 90 100 110 120 130 140 150 160 170 180 a736.pep MKTTEQLEAMNVMAVNPVARWAPRFWAGVFSMPLLASIFNVAGIFGAYLVGVTWLGLDS II II II II II II I II II II II II II I I II I II II I I I I II I I II II I II II I II I I II I I m736 MKTTEQLEAMNVMAVNPVARWAPRFWAGVFSMPLLASIFNVAGIFGAYLVGVTWLGLDS 130 140 150 160 170 180 190 200 210 220 230 240 a736.pep GIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQGFHCVPTSEGILRASTRTWSS I II I II II II I II II II II II I I I II I I II I II II I II II I I I I I II I I I M I M I M II m736 GIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQGFHCVPTSEGILRASTRTWSS 190 200 210 220 230 240 250 259 a736.pep ALTILAVDFILTAWMFTDX II II II II I II II I II II I m736 ALTILAVDFILTAWMFTDX 250 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2509>: g737.seq 1 atgaACATCA AACACCTTCT CTTGACCGCC GCCGCAACCG CACTGTTGGG 51 CATTTCCGCC CCCGCACTCG CCCACCACGA CGGACACGGC GATGACGACC 101 ACGGACACGC CGCACACCAA CACGGCAAAC AAGACAAAAT CATCAGCCGC 151 GCCCAAGCCG AAAAAGCGGC TTGGGCGCGT GTCGGCGGCA AAATCACCGA 201 CATCGATCTC GAACACGACG ACGGCCGTCC GCACTATGAT GTCGAAATCG 251 TCAAAAACGG ACAGGAATAC AAAGTCGTTG TCGATGCCCG TACCGGCCGC 301 GTGATTTCCT CCCGCCGCGA CGACTGA This corresponds to the amino acid sequence <SEQ ID 2510; ORF 737>: g737.ρep 1 MΝIKHLLLTA AATALLGISA PALAHHDGHG DDDHGHAAHQ HGKQDKIISR 51 AQAEKAAWAR VGGKITDIDL EHDDGRPHYD VEIVKΝGQEY KVWDARTGR 101 VISSRRDD* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2511>: m737.seq.. 1 ATGAACATCA AACACCTTCT CTTGACCTCC GCCGCAACCG CACTGCTGAG 51 CATTTCCGCC CCCGCGCTCG CCCACCACGA CGGACACGGC GATGACGACC 101 ACGGACACGC CGCACACCAA CACAACAAAC AAGACAAAAT CATCAGCCGC 151 GCCCAAGCCG AAAAAGCAGC GTTGGCGCGT GTCGGCGGCA AAATCACCGA 201 CATCGATCTC GAACACGACA ACGGCCGTCC GCACTATGAT GTCGAAATCG 251 TCAAAAACGG ACAGGAATAC AAAGTCGTTG TCGATGCCCG TACCGGCCGC 301 GTGATTTCCT CCCGCCGCGA CGACTGA This corresponds to the amino acid sequence <SEQ ID 2512; ORF 737>: m737.pep 1 MΝIKHLLLTS AATALLSISA PALAHHDGHG DDDHGHAAHQ HΝKQDKIISR 51 AQAEKAALAR VGGKITDIDL EHDΝGRPHYD VEIVKΝGQEY KVWDARTGR 101 VISSRRDD* Computer analysis of the amino acid sequences gave the following results: Homology with a predicted ORF from N meningitidis menA with menB ORF 737 shows 95.4% identity over a 108 aa overlap with a predicted ORF (ORF737.a) from N gonorrhoeae: m737/g737 10 20 30 40 50 60 m737.pep MΝIKHLLLTSAATALLSISAPALAHHDGHGDDDHGHAAHQHΝKQDKIISRAQAEKAALAR I I I II I II Ml I II I M I II II I II I II II I II II I II I II: I II I II I I II II I II M g737 MΝIKHLLLTAAATALLGISAPALAHHDGHGDDDHGHAAHQHGKQDKIISRAQAEKAAWAR 10 20 30 40 50 60 70 80 90 100 109 m737. pep VGGKITDIDLEHDΝGRPHYDVEIVKΝGQEYKVWDARTGRVISSRRDDX I I II I M II II I Ml I I II I II I II I I I I II II I II II II I II II I II I g737 VGGKITDIDLEHDDGRPHYDVEIVKΝGQEYKVWDARTGRVISSRRDDX 70 80 90 100 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2513 > • a737.seq 1 ATGAACTTCA AACGCCTTCT CTTGACCGCC GCCGCAACCG CACTGATGGG 51 CATTTCCGCC CCCGCACTCG CCCACCACGA CGGACACGGC GATGACGACC 101 ACGGACACGC CGCACACCAA CACAGCAAAC AAGACAAAAT CATCAGCCGC 151 GCCCAAGCCG AAAAAGCAGC GTTGGCGCGT GTCGGCGGCA AAATCACCGA 201 CATCGATCTC GAACACGACA ACGGCCGTCC GCACTATGAT GTCGAAATCG 251 TCAAAAACGG ACAGGAATAC AAAGTCGTTG TCGATGCCCG TACCGGCCGC 301 GTGATTTCCT CCCGCCGCGA CGACTGA This corresponds to the amino acid sequence <SEQ ID 2514; ORF 737.a>: a737.pep 1 MNFKRLLLTA AATALMGISA PALAHHDGHG DDDHGHAAHQ HSKQDKIISR 51 AQAEKAALAR VGGKITDIDL EHDNGRPHYD VEIVKNGQEY KVWDARTGR 101 VISSRRDD* a737/m737 94.4% identity in 108 aa overlap 10 20 30 40 50 60 a737.pep MNFKRLLLTAAATALMGISAPALAHHDGHGDDDHGHAAHQHSKQDKIISRAQAEKAALAR II: I: II I I : II II MM II II I II I II II I II I II II II M II II II I II I II II I I I I m737 MNIKHLLLTSAATALLSISAPALAHHDGHGDDDHGHAAHQHNKQDKIISRAQAEKAALAR 10 20 30 40 50 60 70 80 90 100 109 a737.pep VGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVWDARTGRVISSRRDDX I I II II II II II II I I II I II I I II I I I I II II II I II I II I I I II II I m737 VGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVWDARTGRVISSRRDDX 70 80 90 100 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2515>: g738.seq 1 ATGTCCGCTG AAACGACCGT ATCCGGCGCG CGCCCCGCCG CCAAACTGCC 51 GATTTACATC CTGCCCTGCT TCCTTTGGAT AGGCATCATC CCCTTTACCT 101 TCGCACTCAG GCTGAAACCG TCGCCCGACT TTTACCACGA TGCCGCCGCC 151 GCGGCCGGCC TGATTGTCCT GTTGTTCCTC ACGGCAGGAA AAAAGCTGTT 201 TGATGTCAAA ATCCCCGCCA TCAGCTTCCT CCTGTTTGCA ATGGCGGCAT 251 TTTGGTGGCT TCAGGCACGC CTGATGAACC TGATTTATCC CGGAATGAAC 301 GACATCGCCT CTTGGGTTTT CATCTTGCTC GCCGTCAGCG CGTGGGCCTG 351 CAAGAGTTTG GTCGCACACT ACGGACAAGA ACGCAtcgtT ACCCTGTTTG 401 CCTGGTCGCT GCTTATCGGC TCCCTGCTTC AATCCTGCAT CGTcgtCATC 451 CAGTTTGCCG GCTGGGAAAA CACCCCCCTG CTTCAAAACA TCATCGTTCA 501 CAGAGGGCAA GGCGTAATCG GACACATCGG GCAGCGCAAC AACCTCGGAC 551 ACTACCTCAT GTGGGGCATA CTCGCCTCCG CCTACCTCAA CGGACAACGA 601 AAAATCCCCG CAGCCCTCGG CGCAATCTGC CTGATTATGC AGACCGCCGT 651 TTTAGGTTTG GTCAATTCGC GCACCATCTT GACCTACATA GCCGCCATCG 701 CCCTCATCCT TCCCTTCTGG TATTTCCGTT CGGACAAATC CAACAGACGG 751 ACGATGCTCG GCATAGCCGC AGCCGTATTC CTTACCGCGC TGTTCCAATT 801 TTCCATGAAC GCCATTCTGG AAACCTTTAC AGGCATCCGC TACGAAACTG 851 CCGTCGAACG CGTCGCCAAC GGCGGTTTCA CAGACTTGCC GCGCCAAAGC 901 GAATGGAATA AAGCCCTTGC CGCCTTCCAG TCCGCCCCGA TATTCGGGCA 951 CGGCTGGAAC AGTTTTGCCC AACAAACCTT CCTGATCAAT GCCGAACAGC 1001 ACACCATACA CGACAACTTC CTCAGCACCT TGTTCACCCA TTCCCACAAC 1051 ATCATCCTCC AACTCCTTGC AGAAATGGGG ATCAGCGGCA CGCTTCTGGT 1101 TGCCGCAACC CTGCTGACGG GCATTGCCGG GCTGCTGAAA CGCTCCCTGA 1151 CCCCCGCATC ACTTTTCCTG CTGTGCGCGC TTGCCGTCAG TATGTGCCAC 1201 AGTATGCTCG AATATCCTTT GTGGTATGTC TATTTCCTCA TCCCCTTCGG 1251 ACTGATGCTC TTTCTGTCCC CCGCAGAGGC TTCAGACGGC ATCGCCTTCA 1301 AAAAAGCCGC CAATCTCGGC ATACTGACCG CCTCCGCCGC CATATTCGCA 1351 GGATTGCTGC ACTTGGACTG GACATACACC CGGCTGGTTA ACTCCTTTTC 1401 CCCCGCCGCT GACGACAGTG CCAAAACCCT CAACCGGAAA ATCAACGAAC 1451 TGCGCTATAT TTCCGCAAAC AGCCCGATGC TGTCCTTTTA TGCCGACTTC 1501 TCCCTCGTAA ACTTCGCCCT GCCGGAATAC CCCGAAACCC AGACTTGGGC 1551 GGAAGAAGCA ACCCTCAAAG CACTAAAATA CCGCCCCTAC TCCGCCACCT 1601 ACCGCATCGC CCTCTACTTG ATGCGGCAAG GCAAAGTTGC AGAAGCAAAA 1651 CAATGGATGC GGGCAACACA GTCCTATTAC CCCTACCTGA TGCCCCGATA 1701 CGCCGACGAA ATCCGCAAAC TGCCCGTATG GGCACCGCTG CTGCCCGAAC 1751 TGCTCAAAGA CTGCAAAGCC TTCGCCGCCG CTCCCGGCCA TCCGGAAACA 1801 AAACCCTGCA AATGA This corresponds to the amino acid sequence <SEQ ID 2516; ORF 738>: g738.pep 1 MSAETTVSGA RPAAKLPIYI LPCFLWIGII PFTFALRLKP SPDFYHDAAA 51 AAGLIVLLFL TAGKKLFDVK IPAISFLLFA MAAFWWLQAR LMNLIYPGMN 101 DIASWVFILL AVSAWACKSL VAHYGQERIV TLFAWSLLIG SLLQSCIWI 151 QFAGWENTPL LQNIIVHRGQ GVIGHIGQRN NLGHYLMWGI LASAYLNGQR 201 KIPAALGAIC LIMQTAVLGL VNSRTILTYI AAIALILPFW YFRSDKSNRR 251 TMLGIAAAVF LTALFQFSMN AILETFTGIR YETAVERVAN GGFTDLPRQS 301 EWNKALAAFQ SAPIFGHGWN SFAQQTFLIN AEQHTIHDNF LSTLFTHSHN 351 IILQLLAEMG ISGTLLVAAT LLTGIAGLLK RSLTPASLFL LCALAVSMCH 401 SMLEYPLWYV YFLIPFGLML FLSPAEASDG IAFKKAANLG ILTASAAIFA 451 GLLHLDWTYT RLVNSFSPAA DDSAKTLNRK INELRYISAN SPMLSFYADF 501 SLVNFALPEY PETQTWAEEA TLKALKYRPY SATYRIALYL MRQGKVAEAK 551 QWMRATQSYY PYLMPRYADE IRKLPVWAPL LPELLKDCKA FAAAPGHPET 601 KPCK* The following partial DNA sequence was identified in N. meningitidis <SEQ TD 2517>: m738 .seq 1 ATGCCCGCTG AAACGACCGT ATCCGGCGCG CACCCCGCCG CCAAACTGCC 51 GATTTACATC CTGCCCTGCT TCCTTTGGAT AGGCATCGTC CCCTTTACCT 101 TCGCGCTCAA ACTGAAACCG TCGCCCGACT TTTACCACGA TGCCGCCGCC 151 GCAGCCGGCC TGATTGTCCT GTTGTTCCTC ACGGCAGGAA AAAAACTGTT 201 TGATGTCAAA ATCCCCGCCA TCAGCTTCCT TCTGTTTGCA ATGGCGGCGT 251 TTTGGTATCT TCAGGCACGC CTGATGAACC TGATTTACCC CGGTATGAAC 301 GACATCGTCT CTTGGATTTT CATCTTGCTC GCCGTCAGCG CGTGGGCCTG 351 CCGGAGCTTG GTCGCACACT TCGGACAAGA ACGCATCGTG ACCCTGTTTG 401 CCTGGTCGCT GCTTATCGGC TCCCTGCTTC AATCCTGCAT CGTCGTCATC 451 CAGTTTGCCG GCTGGGAAGA CACCCCTCTG TTTCAAAACA TCATCGTTTA 501 CAGCGGGCAA GGCGTAATCG GACACATCGG GCAGCGCAAC AACCTCGGAC 551 ACTACCTCAT GTGGGGCATA CTCGCCGCCG CCTACCTCAA CGGACAACGA 601 AAAATCCCCG CCGCCCTCGG CGTAATCTGC CTGATTATGC AGACCGCCGT 651 TTTAGGTTTG GTCAACTCGC GCACCATCTT GACCTACATA GCCGCCATCG 701 CCCTCATCCT TCCCTTCTGG TATTTCCGTT CGGACAAATC CAACAGGCGG 751 ACGATGCTCG GCATAGCCGC AGCCGTATTC CTTACCGCGC TGTTCCAATT 801 TTCCATGAAC ACCATTCTGG AAACCTTTAC TGGCATCCGC TACGAAACTG 851 CCGTCGAACG CGTCGCCAAC GGCGGTTTCA CAGACTTGCC GCGCCAAATC 901 GAATGGAATA AAGCCCTTGC CGCCTTCCAG TCCGCCCCGA TATTCGGGCA 951 CGGCTGGAAC AGTTTTGCCC AACAAACCTT CCTCATCAAT GCCGAACAGC 1001 ACAACATATA CGACAACCTC CTCAGCAACT TGTTCACCCA TTCCCACAAC 1051 ATCGTCCTCC AACTCCTTGC AGAGATGGGA ATCAGCGGCA CGCTTCTGGT 1101 TGCCGCAACC CTGCTGACGG GCATTGCCGG GCTGCTTAAA CGCCCCCTGA 1151 CCCCCGCATC GCTTTTCCTA ATCTGCACGC TTGCCGTCAG TATGTGCCAC 1201 AGTATGCTCG AATATCCTTT GTGGTATGTC TATTTCCTCA TCCCTTTCGG 1251 ACTGATGCTC TTCCTGTCCC CCGCAGAGGC TTCAGACGGC ATCGCCTTCA 1301 AAAAAGCCGC CAATCTCGGC ATACTGACCG CCTCCGCCGC CATATTCGCA 1351 GGATTGCTGC ACTTGGACTG GACATACACC CGGCTGGTTA ACGCCTTTTC 1401 CCCCGCCACT GACGACAGTG CCAAAACCCT CAACCGGAAA ATCAACGAGT 1451 TGCGCTATAT TTCCGCAAAC AGTCCGATGC TGTCCTTTTA TGCCGACTTC 1501 TCCCTCGTAA ACTTCGCCCT GCCGGAATAC CCCGAAACCC AGACTTGGGC 1551 GGAAGAAGCA ACCCTCAAAT CACTAAAATA CCGCCCCCAC TCCGCCACCT 1601 ACCGCATCGC CCTCTACCTG ATGCGGCAAG GCAAAGTTGC AGAAGCAAAA 1651 CAATGGATGC GGGCGACACA GTCCTATTAC CCgTACCTGA TGCCCCGATA 1701 CGCCGACGAA ATCCGCAAAC TGCCCGTATG GGCGCCGCTG CTACCCGAAC 1751 TGCTCAAAGA CTGCAAAGCC TTCGCCGCCG CGCCCGGTCA TCCGGAAGCA 1801 AAACCCTGCA AATGA This corresponds to the amino acid sequence <SEQ ID 2518; ORF 738>: m738.pep 1 MPAETTVSGA HPAAKLPIYI LPCFLWIGIV PFTFALKLKP SPDFYHDAAA 51 AAGLIVLLFL TAGKKLFDVK IPAISFLLFA MAAFWYLQAR LMNLIYPGMN 101 DIVSWIFILL AVSAWACRSL VAHFGQERIV TLFAWSLLIG SLLQSCIVVI 151 QFAGWEDTPL FQNIIVYSGQ GVIGHIGQRN NLGHYLMWGI LAAAYLNGQR 201 KIPAALGVIC LIMQTAVLGL VNSRTILTYI AAIALILPFW YFRSDKSNRR 251 TMLGIAAAVF LTALFQFSMN TILETFTGIR YETAVERVAN GGFTDLPRQI 301 EWNKALAAFQ SAPIFGHGWN SFAQQTFLIN AEQHNIYDNL LSNLFTHSHN 351 IVLQLLAEMG ISGTLLVAAT LLTGIAGLLK RPLTPASLFL ICTLAVSMCH 401 SMLEYPLWYV YFLIPFGLML FLSPAEASDG IAFKKAANLG ILTASAAIFA 451 GLLHLDWTYT RLVNAFSPAT DDSAKTLNRK INELRYISAN SPMLSFYADF 501 SLVNFALPEY PETQTWAEEA TLKSLKYRPH SATYRIALYL MRQGKVAEAK 551 QWMRATQSYY PYLMPRYADE IRKLPVWAPL LPELLKDCKA FAAAPGHPEA 601 KPCK* Computer analysis of the amino acid sequences gave the following results: Homology with a predicted ORF from N meningitidis menA with menB ORF 738 shows 95.0% identity over a 604 aa overlap with a predicted ORF (ORF738.a) from N gonorrhoeae: m738/g738 10 20 30 40 50 60 m738.pep MPAETTVSGAHPAAKLPIYILPCFLWIGIVPFTFALKLKPSPDFYHDAAAAAGLIVLLFL I II II II II : I I II I II II II II I II I M II I II I M II II I II II II II I II II I I I I g738 MSAETTVSGARPAAKLPIYILPCFLWIGIIPFTFALRLKPSPDFYHDAAAAAGLIVLLFL 10 20 30 40 50 60 70 80 90 100 110 120 m738. .pep TAGKKLFDVKIPAISFLLFAMAAFWYLQARLMΝLIYPGMΝDIVSWIFILLAVSAWACRSL II II I II I I II II II II II II I II I : I II II II I II I II II M I M II I II II I I I I : II g738 TAGKKLFDVKIPAISFLLFAMAAFWWLQARLMΝLIYPGMΝDIASWVFILLAVSAWACKSL 70 80 90 100 110 120 130 140 150 160 170 180 m738 • pep VAHFGQERIVTLFAWSLLIGSLLQSCIWIQFAGWEDTPLFQΝIIVYSGQGVIGHIGQRΝ I I I: I I I I II II II I II II I I II II II I II II II IM II I: II II M I I I I II II I I II g738 VAHYGQERIVTLFAWSLLIGSLLQSCIWIQFAGWEΝTPLLQΝIIVHRGQGVIGHIGQRΝ 130 140 150 160 170 180 190 200 210 220 230 240 m738 -pep ΝLGHYLMWGILAAAYLΝGQRKIPAALGVICLIMQTAVLGLVΝSRTILTYIAAIALILPFW I I II I II I II I I I II I II I II II I M I II II II II I II I II II II I I II I II I II I I I g738 ΝLGHYLMWGILASAYLΝGQRKIPAALGAICLIMQTAVLGLVΝSRTILTYIAAIALILPFW 190 200 210 220 230 240 250 260 270 280 290 300 m738.pep YFRSDKSΝRRTMLGIAAAVFLTALFQFSMΝTILETFTGIRYETAVERVAΝGGFTDLPRQI II II II I II II II II I II M I II M II II M II II II I II I I I I II II II II II I II I I g738 YFRSDKSΝRRTMLGIAAAVFLTALFQFSMΝAILETFTGIRYETAVERVAΝGGFTDLPRQS 250 260 270 280 290 300 310 320 330 340 350 360 m738. .pep EWΝKALAAFQSAPIFGHGWΝSFAQQTFLIΝAEQHΝIYDΝLLSΝLFTHSHΝIVLQLLAEMG II II I I II II I M II II I I II I I I II II II II II : M II : I M II II II MM II II I I I g738 EWΝKALAAFQΞAPIFGHGWΝSFAQQTFLIΝAEQHTIHDΝFLSTLFTHSHΝIILQLLAEMG 310 320 330 340 350 360 370 380 390 400 410 420 m738. • pep ISGTLLVAATLLTGIAGLLKRPLTPASLFLICTLAVSMCHSMLEYPLWYVYFLIPFGLML II II I II II II II II II I II I I II II I II : M II II II I II I I II II I II II II II I I I g738 ISGTLLVAATLLTGIAGLLKRSLTPASLFLLCALAVSMCHSMLEYPLWYVYFLIPFGLML 370 380 390 400 410 420 430 440 450 460 470 480 m738, ■ pep FLSPAEASDGIAFKKAAΝLGILTASAAIFAGLLHLDWTYTRLVΝAFSPATDDSAKTLΝRK II I II II I II II I II II II II I II II II II II II I II II I I I I I : I II M I II II II I II g738 FLSPAEASDGIAFKKAAΝLGILTASAAIFAGLLHLDWTYTRLVΝSFSPAADDSAKTLΝRK 430 440 450 460 470 480 490 500 510 520 530 540 m738.pep INELRYISANSPMLSFYADFSLVNFALPEYPETQTWAEEATLKSLKYRPHSATYRIALYL II I II II II II I I II II II II II I II II II II I II II I II I II : II I I I : II II II II II g738 INELRYISANSPMLSFYADFSLVNFALPEYPETQTWAEEATLKALKYRPYSATYRIALYL 490 500 510 520 530 540 550 560 570 580 590 600 m738. pep MRQGKVAEAKQWMRATQSYYPYLMPRYADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEA II I II II II II II I II I II II II II II II II II II II I II II II II I II II I II I II II : g738 MRQGKVAEAKQWMRATQSYYPYLMPRYADEIRKLPVWAPLLPELLKDCKAFAAAPGHPET 550 560 570 580 590 600 m738.pep KPCKX II I II g738 KPCKX The following partial DNA sequence was identified in N meningitidis <SEQ ID 2519>: a738.seq 1 ATGCCCGCTG AAACGACCGT ATCCGGCGCG CACCCCGCCG CCAAACTGCC 51 GATTTACATC CTGCCCTGCT TCCTTTGGAT AGGCATCGTC CCCTTTACCT 101 TTGCGCTCAG GCTGCAACCG TCGCCCGACT TTTACCACGA TGCCGCCGCC 151 GCAGCCGGCC TGATTGTCCT GTTGTTCCTC ACGGCAGGAA AAAAGCTGTT 201 TGATGTCAAA ATCCCACCTA TCAGCTTCCT TCTGTTTGCA ATGGCGGCGT 251 TTTGGTATCT TCAGGCACGC CTGATGAACC TGATTTACCC CGGTATGAAC 301 GACATCGTCT CTTGGATTTT CATCTTACTC GCCGTCAGCG CGTGGGCCTG 351 CCGGAGCTTG GTCGCACACT ACGGACAAGA ACGCATCGTT ACCCTGTTTG 401 CCTGGTCGCT GCTTATCGGC TCCCTGCTTC AATCCTGCAT CGTCGTCATC 451 CAGTTTGCCG GCTGGGAAGA CACCCCTCTG TTTCAAAACA TCATTGTTTA 501 CAGCGGGCAA GGCGTAATCG GACACATCGG ACAGCGCAAC AACCTCGGAC 551 ACTACCTCAT GTGGGGCATA CTCGCCGCCG CCTACCTCAA CGGACAACGA 601 AAAATCCCGC CCGCCTTGGG TGCAATCTGC CTGATTATGC AGACCGCCGT 651 TTTAGGTTTG GTCAATTCGC GCACCATCTT GACCTACATA GCCGCCATCG 701 CCCTCATCCT TCCCTTCTGG TATTTCCGTT CGGACAAATC CAACAGGCGG 751 ACGATACTCG GCATAGCCGC AGCCGTATTC CTTACCGCGC TGTTCCAATT 801 TTCCATGAAC ACCATTCTGG AAACCTTTAC CGGCATCCGC TACGAAACCG 851 CCGTCGAACG CGTCGCCAAC GGCGGTTTCA CAGACCTGCC GCGCCAAATC 901 GAATGGCGCA AAGCCCTCGC CGCCTTCCAG TCCGCCCCGA TATTCGGGCA 951 CGGCTGGAAC AGTTTTGCCC AACAAACCTT CCTCATCAAT GCCGAACAGC 1001 ACAACATACA CGACAACCTC CTCAGCAACT TGTTCACCCA TTCCCACAAC 1051 ATCGTTCTCC AACTCCTTGC AGAGATGGGG ATCAGCGGCA CGCTTCTGGT 1101 TGCCGCAACC CTGCTGACGG GCATTGCCGG GCTGCTGAAA CGCCCCCTGA 1151 CCCCCGCATC GCTTTTCCTG ATCTGCACAC TTGCCGTCAG TATGTGCCAC 1201 AGTATGCTCG AATATCCTTT GTGGTATGTC TATTTCCTCA TCCCCTTCGG 1251 ACTGATGCTC TTTCTGTCCC CCGCAGAGGC TTCAGACGGC ATCGCCTTCA 1301 AAAAAGCCGC CAATCTCGGC ATACTAACCG CCTCCGCCGC CATATTCGCA 1351 GGATTGCTGC ACTTGGACTG GACATACACC CGGATGGTTA ACGCCTTTTC 1401 CCCCGCCACT GACGACAGTG CCAAAACCCT CAACCGGAAA ATCAACGAGT 1451 TGCGCTATAT TTCCGCAAAC AGTCCGATGC TGTCCTTTTA TGCCGACTTC 1501 TCCCTCGTAA ACTTCGCCCT GCCGGAATAC CCCGAAACCC AGACTTGGGC 1551 GGAAGAAGCA ACCCTCAAAT CACTAAAATA CCGCCCCCAC TCCGCCACCT 1601 ACCGCATCGC CCTCTACCTG ATGCGGCAAG GCAAAGTTGC AGAAGCAAAA 1651 CAATGGATGC GGGCGACACA GTCCTATTAC CCCTACCTGA TGCCCCGATA 1701 CGCCGACGAA ATCCGCAAAC TGCCCGTATG GGCGCCGCTG CTACCCGAAC 1751 TGCTCAAAGA CTGCAAAGCC TTCGCCGCCG CGCCCGGTCA TCCGGAAGCA 1801 AAACCCTGCA AATGA This corresponds to the amino acid sequence <SEQ ID 2520; ORF 738. a>: a738.pep 1 MPAETTVSGA HPAAKLPIYI LPCFLWIGIV PFTFALRLQP SPDFYHDAAA 51 AAGLIVLLFL TAGKKLFDVK IPPISFLLFA MAAFWYLQAR LMΝLIYPGMΝ 101 DIVSWIFILL AVSAWACRSL VAHYGQERIV TLFAWSLLIG SLLQSCIWI 151 QFAGWEDTPL FQΝIIVYSGQ GVIGHIGQRΝ ΝLGHYLMWGI LAAAYLΝGQR 201 KIPPALGAIC LIMQTAVLGL VNSRTILTYI AAIALILPFW YFRSDKSNRR 251 TILGIAAAVF LTALFQFSMN TILETFTGIR YETAVERVAN GGFTDLPRQI 301 EWRKALAAFQ SAPIFGHGWN SFAQQTFLIN AEQHNIHDNL LSNLFTHSHN 351 IVLQLLAEMG ISGTLLVAAT LLTGIAGLLK RPLTPASLFL ICTLAVSMCH 401 SMLEYPLWYV YFLIPFGLML FLSPAEASDG IAFKKAANLG ILTASAAIFA 451 GLLHLDWTYT RMVNAFSPAT DDSAKTLNRK INELRYISAN SPMLSFYADF 501 SLVNFALPEY PETQTWAEEA TLKSLKYRPH SATYRIALYL MRQGKVAEAK 551 QWMRATQSYY PYLMPRYADE IRKLPVWAPL LPELLKDCKA FAAAPGHPEA 601 KPCK* a738/m738 98.3% identity in 604 aa overlap 10 20 30 40 50 60 a738.pep MPAETTVSGAHPAAKLPIYILPCFLWIGIVPFTFALRLQPSPDFYHDAAAAAGLIVLLFL I II I II II I II II II II II II II I I II I II I II II I : I : II II II I II II I I I II II I II m738 MPAETTVSGAHPAAKLPIYILPCFLWIGIVPFTFALKLKPSPDFYHDAAAAAGLIVLLFL 10 20 30 40 50 60 70 80 90 100 110 120 a738.pep TAGKKLFDVKIPPISFLLFAMAAFWYLQARLMNLIYPGMNDIVSWIFILLAVSAWACRSL I II II II I II II I I II II II II II II II I II II II II I I II II I I II I II I II II II II m738 TAGKKLFDVKIPAISFLLFAMAAFWYLQARLMNLIYPGMNDIVSWIFILLAVSAWACRSL 70 80 90 100 110 120 130 140 150 160 170 180 a738.pep VAHYGQERIVTLFAWSLLIGSLLQSCIWIQFAGWEDTPLFQNIIVYSGQGVIGHIGQRN I I I : II II II I II I II II II I II I I I I II II II II II II I II I II I II II I I I II II II I m738 VAHFGQERIVTLFAWSLLIGSLLQSCIWIQFAGWEDTPLFQNIIVYSGQGVIGHIGQRN 130 140 150 160 170 180 190 200 210 220 230 240 a738.pep NLGHYLMWGILAAAYLNGQRKIPPALGAICLIMQTAVLGLVNSRTILTYIAAIALILPFW II I II II II I II II II II II I II I II : II II II II II I II II II I II II I I II II I I II m738 NLGHYLMWGILAAAYLNGQRKIPAALGVICLIMQTAVLGLVNSRTILTYIAAIALILPFW 190 200 210 220 230 240 250 260 270 280 290 300 a738.pep YFRSDKSNRRTILGIAAAVFLTALFQFSMNTILETFTGIRYETAVERVANGGFTDLPRQI II II II I II I I : II I II II II II II I I I II II II I II II II I II II I II I I I II II II II m738 YFRSDKSNRRTMLGIAAAVFLTALFQFSMNTILETFTGIRYETAVERVANGGFTDLPRQI 250 260 270 280 290 300 310 320 330 340 350 360 a738.pep EWRKALAAFQSAPIFGHGWNSFAQQTFLINAEQHNIHDNLLSNLFTHSHNIVLQLLAEMG II I I I II I II I II II II II II II I II II I II II II : I II II II I I II II I I II I II I II m738 EWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQHNIYDNLLSNLFTHSHNIVLQLLAEMG 310 320 330 340 350 360 370 380 390 400 410 420 a738.pep ISGTLLVAATLLTGIAGLLKRPLTPAΞLFLICTLAVSMCHSMLEYPLWYVYFLIPFGLML I I II II II II II II II II I I II II I II II II I I II I II II I I II I II I I II II I I I II II m738 ISGTLLVAATLLTGIAGLLKRPLTPASLFLICTLAVSMCHSMLEYPLWYVYFLIPFGLML 370 380 390 400 410 420 430 440 450 460 470 480 a738.pep FLSPAEASDGIAFKKAANLGILTASAAIFAGLLHLDWTYTRMVNAFSPATDDSAKTLNRK MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM m738 FLSPAEASDGIAFKKAANLGILTASAAIFAGLLHLDWTYTRLVNAFSPATDDSAKTLNRK 430 440 450 460 470 480 490 500 510 520 530 540 a738.pep INELRYISANSPMLSFYADFSLVNFALPEYPETQTWAEEATLKSLKYRPHSATYRIALYL MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM m738 INELRYISANSPMLSFYADFSLVNFALPEYPETQTWAEEATLKSLKYRPHSATYRIALYL 490 500 510 520 530 540 550 560 570 580 590 600 a738. pep MRQGKVAEAKQWMRATQSYYPYLMPRYADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEA II I II II I I I II II I I I II II II II II II II I II II I II II II I II I II II I I I II II II m738 MRQGKVAEAKQWMRATQSYYPYLMPRYADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEA 550 560 570 580 590 600 a738.pep KPCKX II II I m738 KPCKX The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2521>: g739.seq 1 ATGGCAAAAA AACCGAACAA ACCCTTCAGG CTGACCCCCA AACTCCTGAT 51 ACGCGCCGTA TTGCTCATCT GTATCACCGC CATCGGCGCA TTGGCAGTAG 101 GCATCGTCAG CACATTCAAC CCGAACGGCG ACAAAACCCT CCAAACCGAA 151 CCGCAACACA CCGACAGCCC CCGCGAAACC GAATTCTGGC TGCCAAACGG 201 CGCCGTCGGA CAAGATGCCG CCCAACCCGA ACACCACCAC GCCGCCTCAT 251 CCGAACCCGC ACAGCCGGAC GGCACAGAAG AAAGCGGCAG CGGACTGCCG 301 TCCCCTGCCG CACCCAAGAA AAACCGGGTc AAACCGCGCC CTTCGGATGC 351 GGCCCGGGCA GCCGATTCGT TAACCGGCAC CGGAACACAA GCTGAAAACA 401 CACTCAAAGA AACCCCCGTA CTGCCCACAA ACGCCCCCCA TCCCGAACCC 451 CGAAAAGAAA CACCCGAAAA ACAGGCGCAG CCCAAAGAAA CACCCAAAGA 501 AAAAGAAACG CCCAAAGAAA ACCATACCAA ACCGGACACC CCGAAAAACA 551 CGCCGGCCAA ACCCCATAAA GAGATTCTCG ACAACCTCTT TTGA This corresponds to the amino acid sequence <SEQ ID 2522; ORF 739>: g739.pep 1 MAKKPΝKPFR LTPKLLIRAV LLICITAIGA LAVGIVSTFΝ PΝGDKTLQTE 51 PQHTDSPRET EFWLPΝGAVG QDAAQPEHHH AASSEPAQPD GTEESGSGLP 101 SPAAPKKΝRV KPRPSDAARA ADSLTGTGTQ AEΝTLKETPV LPTΝAPHPEP 151 RKETPEKQAQ PKETPKEKET PKEΝHTKPDT PKΝTPAKPHK EILDΝLF* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2523>: m739.seq 1 ATGGCAAAAA AACCGAACAA ACCCTTCAGG CTGACCCCCA AACTCCTGAT 51 ACGCGCCGTA TTGCTCATCT GTATCGCCGC CATCGGCGCA TTGGCAATAG 101 GCATCGTCAG CACATTCAAC CCGAACGGCG ACAAAACCCT TCAAGCCGAA 151 CCGCAACACA CCGACAGCCC CCGCGAAACC GAATTCTGGC TGCCAAACGG 201 CGTAGTCGGA CAAGATGCCG CCCAACCCGA ACACCACCAC GCCGCCTCAT 251 CCGAACCCGC ACAGCCGGAC GGCACAGACG AAAGCGGCAG CGGACTGCCG 301 TCCCCTGCCG CACCCAAGAA AAACCGGGTC AAACCGCAAC CTGCCGACAC 351 AGCTCAAACC GACAGGCAGC CGGACGACGC CGGAACACAA GCTGAAAACA 401 CACTCAAAGA AACCCCCGTA CTGCCCACAA ACGTCCCCCG TCCCGAACCC 451 CGAAAAGAAA CACCCGAAAA ACAGGCGCAG CCCAAAGAAA CGCCCAAAGA 501 AAACCATACC AAACCGGACA CCCCGAAAAA CACGCCGCCC AAACCCCATA 551 AAGAAATTCT CGACAAACTC TTC This corresponds to the amino acid sequence <SEQ ID 2524; ORF 739>: m739.pep 1 MAKKPΝKPFR LTPKLLIRAV LLICIAAIGA LAIGIVSTFΝ PΝGDKTLQAE 51 PQHTDSPRET EFWLPΝG G QDAAQPEHHH AASSEPAQPD GTDESGSGLP 101 SPAAPKKΝRV KPQPADTAQT DRQPDDAGTQ AEΝTLKETPV LPTΝVPRPEP 151 RKETPEKQAQ PKETPKEΝHT KPDTPKΝTPP KPHKEILDKL F Computer analysis of the amino acid sequences gave the following results: Homology with a predicted ORF from N meningitidis menA with menB ORF 739 shows 86.3% identity over a 197 aa overlap with a predicted ORF (ORF739.a) from N. gonorrhoeae: m739/g739 10 20 30 40 50 60 m739.pep MAKKPNKPFRLTPKLLIRAVLLICIAAIGALAIGIVSTFNPNGDKTLQAEPQHTDSPRET I I I I II II II I II II I I II II I II I : II I I II : I I II I II I II II II I : I II I II II II I g 739 MAKKPNKPFRLTPKLLIRAVLLICITAIGALAVGIVSTFNPNGDKTLQTEPQHTDSPRET 10 20 30 40 50 60 70 80 90 100 110 120 m739 . pep EFWLPNGWGQDAAQPEHHHAASSEPAQPDGTDESGSGLPSPAAPKKNRVKPQPADTAQT I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I : I : I : I : : g739 EFWLPNGAVGQDAAQPEHHHAASSEPAQPDGTEESGSGLPSPAAPKKNRVKPRPSDAARA 70 80 90 100 110 120 130 140 150 160 170 m739 . pep DRQPDDAGTQAENTLKETPVLPTNVPRPEPRKETPEKQAQPKETPKE NHTKPDT : : I II II I II I I I II I II M M II I II I II I I II II I II II I II I I I II g 739 ADSLTGTGTQAENTLKETPVLPTNAPHPEPRKETPEKQAQPKETPKEKETPKENHTKPDT 130 140 150 160 170 180 180 190 m739.pep PKNTPPKPHKEILDKLF Mill II II I II I : II g 739 PKNTPAKPHKEILDNLFX 190 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2525>: a739.seq 1 ATGGCAAAAA AACCGAACAA ACCCTTCAGG CTGACCCCCA AACTCCTGAT 51 ACGCGCCGTA TTGCTCATCT GTATCACCGC CATCGGCGCA TTGGCAATAG 101 GCATCGTCAG CACATTCAAC CCGAACGGCG ACAAAACCCT CCAAACCGAA 151 CCGCAACACA CCGACAGCCC CCGCGAAACC GAATTCTGGC TGCCAAACGG 201 CGTAGTCGGA CAAGATGCCG CCCAACCCGA ACACCACCAC GCCTCCTCAT 251 CCGCACCCGC ACAGCCGGAC GGCACAGACG AAAGCGGCAG CGGACTGCCG 301 TCCCCTGCCG CACCCAAGAA AAACCGGGTC AAACCGCAAC CTGCCGACAC 351 AGCTCAAACC GACAGGCAGC CGGACGACGC CGGAGCACAA GCTGAAAACA 401 CACTCAAAGA AACCCCCGTA CTGCCCACAA ACGTCCCCCG TCCCGAACCC 451 CGAAAAGAAA CACCCGAAAA ACAGGCACAG CCCAAAGAAA CACCCAAAGA 501 AAAAGAAACG CCCAAAGAAA ACCATACCAA ACCGGACACC CCGAAAAACA 551 CGCCGCCTAA ACCCCATAAA GAAATTCTCG ACAACCTCTT CTGA This corresponds to the amino acid sequence <SEQ ID 2526; ORF 739.a>: a739.pep 1 MAKKPΝKPFR LTPKLLIRAV LLICITAIGA LAIGIVSTFΝ PΝGDKTLQTE 51 PQHTDSPRET EFWLPΝGWG QDAAQPEHHH ASSSAPAQPD GTDESGSGLP 101 SPAAPKKΝRV KPQPADTAQT DRQPDDAGAQ AEΝTLKETPV LPTΝVPRPEP 151 RKETPEKQAQ PKETPKEKET PKEΝHTKPDT PKΝTPPKPHK EILDΝLF* a739/m739 93.9% identity in 197 aa overlap 10 20 30 40 50 60 a739.pep MAKKPΝKPFRLTPKLLIRAVLLICITAIGALAIGIVSTFΝPΝGDKTLQTEPQHTDSPRET II I II II II I II II I I I I II I II II : I II II II II I I I II I I II I II I : I I II I I I I I II m739 MAKKPΝKPFRLTPKLLIRAVLLICIAAIGALAIGIVSTFΝPΝGDKTLQAEPQHTDSPRET 10 20 30 40 50 60 70 80 90 100 110 120 a739.pep EFWLPΝGWGQDAAQPEHHHASSSAPAQPDGTDESGSGLPSPAAPKKΝRVKPQPADTAQT I II I II II II I I II II II I II : I I I I II I I II I I I II II II II I II I II II II I I II I I m739 EFWLPΝGWGQDAAQPEHHHAASSEPAQPDGTDESGSGLPSPAAPKKΝRVKPQPADTAQT 70 80 90 100 110 120 130 140 150 160 170 180 a739. pep DRQPDDAGAQAEΝTLKETPVLPTΝVPRPEPRKETPEKQAQPKETPKEKETPKEΝHTKPDT I II II I II : II II II II II I II I II II II I II I I II I I I II II I II II II II II m739 DRQPDDAGTQAEΝTLKETPVLPTΝVPRPEPRKETPEKQAQPKETPK EΝHTKPDT 130 140 150 160 170 190 a739.pep PKNTPPKPHKEILDNLFX I II II II II II I II : I I m739 PKNTPPKPHKEILDKLF 180 190 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2527>: g740.seq 1 ATGTCCCGAA ACCTGCTTGT CCGCTGGCTC GCCGTCTGCC TCATCCCCTT 51 GgcgACGCTT GCCGTTTTCG CCGCCAATcc gcCCGAAGAC AAACCCCAGC 101 ATCTGATCAA CGGCATCATC CTTGCCTGCG AAGCGACGTT TTTGTTTAAa 151 ttcgtgctCT TTGAAACCAT CAAGCATCAT CTTAaacaag gGTTTGATTT 201 GAAACgtcaa ACCATGTTTC TGTTTATTCC GATTGTTTTG CTGGTTGTGT 251 ATTTGTTCCA CTATTTCGGC GCGTTTTag This corresponds to the amino acid sequence <SEQ ID 2528; ORF 740.ng>: g740.pep 1 MΞRΝLLVRWL AVCLIPLATL AVFAAΝPPED KPQHLIΝGII LACEATFLFK 51 FVLFETIKHH LKQGFDLKRQ TMFLFIPIVL LVVYLFHYFG AF* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2529>: m740.seq 1 ATGTCCCGAA ACCTGCTTGT CCGCTGGCTT GCCGTCTGCC TCATCCCGTT 51 GGCGACGCTT GCCGTTTTCG CCGCCAATCC GCCCGAAGAC AAACTCCAGC 101 ATCTGATCAA CGGCATCATC CTTGCCTGCG AAGCGACGTT TTTGTTTAAA 151 TTCGTCCTTT TCGACACCAT CAAGCATCAT TTGAAACAAG AGTTTGATTT 201 GAAACGTCAA ACTATGTTGC TGTTTATTCC GATTATTTTG CTGATTGTGT 251 ATTTGTTCCA CTATTTTGGC GCGTTTTAG This corresponds to the amino acid sequence <SEQ ID 2530; ORF 740>: m740.pep 1 MSRΝLLVRWL AVCLIPLATL AVFAAΝPPED KLQHLIΝGII LACEATFLFK 51 FVLFDTIKHH LKQEFDLKRQ TMLLFIPIIL LIVYLFHYFG AF* m740/g740 93.5% identity in 92 aa overlap 10 20 30 40 50 60 m740.pep MSRΝLLVRWLAVCLIPLATLAVFAAΝPPEDKLQHLIΝGIILACEATFLFKFVLFDTIKHH I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I Ml I I I I g740 MSRΝLLVRWLAVCLIPLATLAVFAAΝPPEDKPQHLIΝGIILACEATFLFKFVLFETIKHH 10 20 30 40 50 60 70 80 90 m740.pep LKQEFDLKRQTMLLFIPIILLIVYLFHYFGAFX III II I I I I I M I I I I M I MM I I I I I I I I I g740 LKQGFDLKRQTMFLFIPIVLLVVYLFHYFGAFX 70 80 90 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2531>: a740.seq 1 ATGTCCCGAA ACCTGCTTGT CCGCTGGCTT GTCGTCTGCC TGATACCCTT 51 GGCGACGCTT GCCGTTTTCG CCGCCAATCC GCCCGAAGAC AAACCCCAGC 101 ATCTGATTAA CGGCATCATC CTTGCCTGCG AAGCGACGTT TTTGTTCAAA 151 TTCGTCCTTT TCGACACCAT CAAGCATCAT TTGAAACAAG AGTTTGATTT 201 GAAACGTCAA ACTATGTTGC TGTTTATTCC GATTATTTTG CTGATTGTGT 251 ATTTGTTCCA CTATTTTGGC GCGTTTTAG This corresponds to the amino acid sequence <SEQ ID 2532; ORF 740. a>: a740.pep 1 MSRΝLLVRWL WCLIPLATL AVFAAΝPPED KPQHLIΝGII LACEATFLFK 51 FVLFDTIKHH LKQEFDLKRQ TMLLFIPIIL LIVYLFHYFG AF* a740/m740 97.8% identity in 92 aa overlap 10 20 3 0 40 50 60 a740 . pep MSRNLLVRWLVVCLIPLATLAVFAANPPEDKPQHLINGIILACEATFLFKFVLFDTIKHH I I I I I I I I I I : I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m7 0 MSRNLLVRWLAVCLIPLATLAVFAANPPEDKLQHLINGIILACEATFLFKFVLFDTIKHH 10 20 30 40 50 60 70 80 90 a740.pep LKQEFDLKRQTMLLFIPIILLIVYLFHYFGAFX I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I m7 40 LKQEFDLKRQTMLLFIPIILLIVYLFHYFGAFX 70 80 90 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2533>: g741.seq 1 GTGAACCGAA CTACCTTCTG CTGCCTTTCT TTGACCGCCG GCCCTGATTC 51 TGACCGCCTG CAGCAGCGGA GGGGCGGAGG CGGTGGTGTC GCCGCCGACA 101 TCGGCACGGG GCTTGCCGAT GCATTAACCG CGCCGCTCGA CCATAAAGAC 151 AAAGGTTTGA AATCCCTAAC ATTGGAAGCC TCCATTCCCC AAAACGGAAC 201 ACTGACCCTG TCGGCACAAG GTGCGGAAAA AACTTTCAAA GCCGGCGGCA 251 AAGACAACAG CCTCAACACG GGCAAACTGA AGAACGACAA AATCAGCCGC 301 TTCGACTTCG TGCAAAAAAT CGAAGTGGAC GGACAAACCA TCACACTGGC 351 AAGCGGCGAA TTTCAAATAT ACAAACAGGA TCACTCCGcc gtcgtTgcCC 401 TacgGATTGA AAAAATCAAC AACCCCGACA AAATCGACAG CCTGATAAAC 451 CAACGCTCCT TCCTTGTCAG CGATTTGGGC GGAGAACATA CCGCCTTCAA 501 CCAACTGCCT GACGGCAAAG CCGAGTATCA CGGCAAAGCA TTCAGCTCCG 551 ACGATGCCGA CGGAAAACTG ACCTATACCA TAGATTTCGC CGCCAAACAG 601 GGACACGGCA AAATCGAACA CCTGAAAACA CCCGAGCAGA ATGTTGAGCT 651 TGCCTCCGCC GAACTCAAAG CAGATGAAAA ATCACACGCC GTCATTTTGG 701 GCGACACGCG CTACGGCGGC GAAGAGAAAG GCACTTACCG CCTCGCCCTT 751 TTCGGCGACC GCGCCCAAGA AATCGCTGGC TCGGCAACCG TGAAGATAGG 801 GGAAAAGGTT CACGAAATCG GCATCGCCGA CAAACAGTAG This corresponds to the amino acid sequence <SEQ ID 2534; ORF 741. ng>: g7 1.pep 1 VΝRTTFCCLS LTAGPDSDRL QQRRGGGGGV AADIGTGLAD ALTAPLDHKD 51 KGLKSLTLEA SIPQΝGTLTL SAQGAEKTFK AGGKDΝSLΝT GKLKΝDKISR 101 FDFVQKIEVD GQTITLASGE FQIYKQDHSA WALRIEKIΝ ΝPDKIDSLIΝ 151 QRSFLVSDLG GEHTAFΝQLP DGKAEYHGKA FSSDDADGKL TYTIDFAAKQ 201 GHGKIEHLKT PEQΝVELASA ELKADEKSHA VILGDTRYGG EEKGTYRLAL 251 FGDRAQEIAG SATVKIGEKV HEIGIADKQ* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2535>: m741.seq 1 GTGAATCGAA CTGCCTTCTG CTGCCTTTCT CTGACCACTG CCCTGATTCT 51 GACCGCCTGC AGCAGCGGAG GGGGTGGTGT CGCCGCCGAC ATCGGTGCGG 101 GGCTTGCCGA TGCACTAACC GCACCGCTCG ACCATAAAGA CAAAGGTTTG 151 CAGTCTTTGA CGCTGGATCA GTCCGTCAGG AAAAACGAGA AACTGAAGCT 201 GGCGGCACAA GGTGCGGAAA AAACTTATGG AAACGGTGAC AGCCTCAATA 251 CGGGCAAATT GAAGAACGAC AAGGTCAGCC GTTTCGACTT TATCCGCCAA 301 ATCGAAGTGG ACGGGCAGCT CATTACCTTG GAGAGTGGAG AGTTCCAAGT 351 ATACAAACAA AGCCATTCCG CCTTAACCGC CTTTCAGACC GAGCAAATAC 401 AAGATTCGGA GCATTCCGGG AAGATGGTTG CGAAACGCCA GTTCAGAATC 451 GGCGACATAG CGGGCGAACA TACATCTTTT GACAAGCTTC CCGAAGGCGG 501 CAGGGCGACA TATCGCGGGA CGGCGTTCGG TTCAGACGAT GCCGGCGGAA 551 AACTGACCTA CACCATAGAT TTCGCCGCCA AGCAGGGAAA CGGCAAAATC 601 GAACATTTGA AATCGCCAGA ACTCAATGTC GACCTGGCCG CCGCCGATAT 651 CAAGCCGGAT GGAAAACGCC ATGCCGTCAT CAGCGGTTCC GTCCTTTACA 701 ACCAAGCCGA GAAAGGCAGT TACTCCCTCG GTATCTTTGG CGGAAAAGCC 751 CAGGAAGTTG CCGGCAGCGC GGAAGTGAAA ACCGTAAACG GCATACGCCA 801 TATCGGCCTT GCCGCCAAGC AATAA This corresponds to the amino acid sequence <SEQ ID 2536; ORF 741>: m741.pep 1 VΝRTAFCCLS LTTALILTAC SSGGGGVAAD IGAGLADALT APLDHKDKGL 51 QSLTLDQSVR KΝEKLKLAAQ GAEKTYGΝGD SLΝTGKLKΝD KVSRFDFIRQ 101 IEVDGQLITL ESGEFQVYKQ SHSALTAFQT EQIQDSEHSG KMVAKRQFRI 151 GDIAGEHTSF DKLPEGGRAT YRGTAFGSDD AGGKLTYTID FAAKQGNGKI 201 EHLKSPELNV DLAAADIKPD GKRHAVISGS VLYNQAEKGS YSLGIFGGKA 251 QEVAGSAEVK TVNGIRHIGL AAKQ* m741/g741 61.4% identity in 280 aa overlap 10 20 30 40 50 m741.pep VNRTAFCCLSLTT ALILTACSSGGGGVAADIGAGLADALTAPLDHKDKGLQSLTLDQ II I I : I II II I I : : I M II I II II I M II II I II II II II I II I : II I M g741 VNRTTFCCLSLTAGPDSDRLQQRRGGGGGVAADIGTGLADALTAPLDHKDKGLKSLTLEA 10 20 30 40 50 60 60 70 80 90 100 110 m741.pep SVRKNEKLKLAAQGAEKTY GNGDSLNTGKLKNDKVSRFDFIRQIEVDGQLITLESGE M M I I : II I II I I : M : I II I II I I II I : II I II : : : I I II I I III III g741 SIPQNGTLTLSAQGAEKTFKAGGKDNSLNTGKLKNDKISRFDFVQKIEVDGQTITLASGE 70 80 90 100 110 120 120 130 140 150 160 170 m741. pep FQVYKQSHSALTAFQTEQIQDSEHSGKMVAKRQFRIGDIAGEHTSFDKLPEGGRATYRGT I I : I I |: I I I :: I :: MM: :: ::: : I : I : : I : : | I I I : |: : | | : | : | Ml g741 FQIYKQDHSAWALRIEKINNPDKIDSLINQRSFLVSDLGGEHTAFNQLPDG-KAEYHGK 130 140 150 160 170 180 190 200 210 220 230 m741.pep AFGSDDAGGKLTYTIDFAAKQGNGKIEHLKSPELNVDLAAADIKPDGKRHAVISGSVLYN I I : II II II I II I II II I II I : II II I II : II I I : I I : I : : I I I llll I : : I : g741 AFSSDDADGKLTYTIDFAAKQGHGKIEHLKTPEQNVELASAELKADEKSHAVILGDTRYG 180 190 200 210 220 230 240 250 260 270 m741. pep QAEKGSYSLGIFGGKAQEVAGSAEVKTVNGIRHIGLAAKQX II M I I :: I I : I II : I II I II : ::: I I : I III g741 GEEKGTYRLALFGDRAQEIAGSATVKIGEKVHEIGIADKQX 240 250 260 270 280 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2537>: a741. seq 1 GTGAACCGAA CTGCCTTCTG CTGCCTTTCT TTGACCGCCG CCCTGATTCT 51 GACCGCCTGC AGCAGCGGAG GCGGCGGTGT CGCCGCCGAC ATCGGCGCGG 101 TGCTTGCCGA TGCACTAACC GCACCGCTCG ACCATAAAGA CAAAAGTTTG 151 CAGTCTTTGA CGCTGGATCA GTCCGTCAGG AAAAACGAGA AACTGAAGCT 201 GGCGGCACAA GGTGCGGAAA AAACTTATGG AAACGGCGAC AGCCTCAATA 251 CGGGCAAATT GAAGAACGAC AAGGTCAGCC GCTTCGACTT TATCCGTCAA 301 ATCGAAGTGG ACGGGCAGCT CATTACCTTG GAGAGCGGAG AGTTCCAAGT 351 GTACAAACAA AGCCATTCCG CCTTAACCGC CCTTCAGACC GAGCAAGTAC 401 AAGATTCGGA GCATTCAGGG AAGATGGTTG CGAAACGCCA GTTCAGAATC 451 GGCGATATAG CGGGTGAACA TACATCTTTT GACAAGCTTC CCGAAGGCGG 501 CAGGGCGACA TATCGCGGGA CGGCATTCGG TTCAGACGAT GCCAGTGGAA 551 AACTGACCTA CACCATAGAT TTCGCCGCCA AGCAGGGACA CGGCAAAATC 601 GAACATTTGA AATCGCCAGA ACTCAATGTT GACCTGGCCG CCTCCGATAT 651 CAAGCCGGAT AAAAAACGCC ATGCCGTCAT CAGCGGTTCC GTCCTTTACA 701 ACCAAGCCGA GAAAGGCAGT TACTCTCTAG GCATCTTTGG CGGGCAAGCC 751 CAGGAAGTTG CCGGCAGCGC AGAAGTGGAA ACCGCAAACG GCATACGCCA 801 TATCGGTCTT GCCGCCAAGC AGTAA This corresponds to the amino acid sequence <SEQ ID 2538; ORF 741.a>: a7 1.pep 1 VΝRTAFCCLS LTAALILTAC SSGGGGVAAD IGAVLADALT APLDHKDKSL 51 QSLTLDQSVR KΝEKLKLAAQ GAEKTYGΝGD SLΝTGKLKΝD KVSRFDFIRQ 101 IEVDGQLITL ESGEFQVYKQ SHSALTALQT EQVQDSEHSG KMVAKRQFRI 151 GDIAGEHTSF DKLPEGGRAT YRGTAFGSDD ASGKLTYTID FAAKQGHGKI 201 EHLKSPELNV DLAASDIKPD KKRHAVISGS VLYNQAEKGS YSLGIFGGQA 251 QEVAGSAEVE TANGIRHIGL AAKQ* a741/m741 95.6% identity in 274 aa overlap 10 20 30 40 50 60 a741.pep VNRTAFCCLSLTAALILTACSSGGGGVAADIGAVLADALTAPLDHKDKSLQSLTLDQSVR II I II I II I II I : I II I II I I I I II II I I I II I II I II I II II II I I : I II II I II I II m741 VNRTAFCCLSLTTALILTACSSGGGGVAADIGAGLADALTAPLDHKDKGLQSLTLDQSVR 10 20 30 40 50 60 70 80 90 100 110 120 a741. pep KNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIEVDGQLITLESGEFQVYKQ II II I I II I II II I I II II II II II II II I II I I II I II II II I II II I II II I II I II I m741 KNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIEVDGQLITLESGEFQVYKQ 70 80 90 100 110 120 130 140 150 160 170 180 a741.pep SHSALTALQTEQVQDSEHSGKMVAKRQFRIGDIAGEHTSFDKLPEGGRATYRGTAFGSDD II II II I : II II : II I II II II I I II I II I I I II II II II I I II II II I I I II II II II I m741 SHSALTAFQTEQIQDSEHΞGKMVAKRQFRIGDIAGEHTSFDKLPEGGRATYRGTAFGSDD 130 140 150 160 170 180 190 200 210 220 230 240 a741.pep ASGKLTYTIDFAAKQGHGKIEHLKSPELNVDLAASDIKPDKKRHAVISGSVLYNQAEKGS I : II II II II I II II I: II II II II I II II II II : II I II II II I II I I I I II II II II m741 AGGKLTYTIDFAAKQGNGKIEHLKSPELNVDLAAADIKPDGKRHAVISGSVLYNQAEKGS 190 200 210 220 230 240 250 260 270 a7 1.pep YSLGIFGGQAQEVAGSAEVETANGIRHIGLAAKQX I II I II II : II I II II I II : I : I II II II I I II I I m741 YSLGIFGGKAQEVAGSAEVKTVNGIRHIGLAAKQX 250 260 270 g742.seq not found yet g742.pep not found yet The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2539>: m742. seq 1 ATGGTTTACG GCATTGCCGA AGCCGATGCG GGCGACAGCA GTGTGCTTAC 51 TTTGGGCGGC ATGTATCAGA AGAGTAGGGA GGTTCCTGAT TTTTCGGGCA 101 TTATTTTGCC CTGTGAAAAT CAGAAAACTG CCCCGTTCAG TTCAACGCCT 151 GCCTGCAACC GGCCTTTGCA ACTGCCGCGC AACACTTATT TGGGGGAGGA 201 TTGGTCGCGG TTAAGTGCCG ACAAATACAA CCTTTTCTCA GGATTCAAAC 251 ATGTGTTTGA CAACGGTTGG CAGCTCAATG CCGAAGTGTC TTATACCAAG 301 AATGAATCCG ATGCGAAGGT GGGGCAGTTT TTTCTGAAAA ACGAATATGC 351 GGCGGGTTTG TCGGGTGAGG ATGCGGTAGG CTTTTTGACT GAAAAAAACG 401 AAGTCATCCC GTTCGAGCCG AAAGATAAGG CATTGGAGAA ACTGAAAGCA 451 TATCGTGATG AAACCGCCAA GGAATACCGG GAGCGCAAAG ACGATTTTGT 501 TAAAAACCGT TTCGATAATA CTGCTTTCGA ACAGTATCGC AGCCGCCGTG 551 CCGCAGAACG CAAAGCCGGT TTTGACAAGT GTATGAGTGA CCCTTTCGCG 601 CTGGACTTTA TCTGTCAAGG TTCTTGGGGG GATCCGGGCG TTGATGCCGA 651 CAAGGCGGAA TTTGTCGATA AAGCCCTTGC GAAGGAGGGC ATCTTTAATA 701 ATGCGGCACA ACGTTTTCCA AACAGCCTGT ATGACTCTTC CTTTAATCGG 751 AAGGCTACCG CCAACCGACG ATACAGTTAT ATGCCGTTGC GGCATACCAA 801 AGACGACCGC CAATGGGGAA TTAAACTTGA CCTGACCGGC ACATATGGGC 851 TGTTCGGGCG GGAGCATGAT TTCTTTGTCG GCTATGCCTA CGGTGATGAA 901 AAGATACGTT CGGAATATCT AGAAATCTAC GAACGCCGCT ACAGAGTACG 951 TCCGAATACG GGGGCAACGC ACGGCGTGTA TGCGGGAAGT TGTCAGGAGG 1001 AGCCGGACGG CGATTTGTCG TCTCCTTTGG TCAGGGGGCA TAAAGAACCC 1051 GATTGGCAGG CGTACGATGA AAAAGGCAAC CGTACCGTTT ATGCCGAAGA 1101 ATGCAGGAAC GCCAAGAAAA TAAAAACCGA GCCCAAGCTC GATGCCGAAG 1151 GCAAGCAGGT GTATTACTAT GACGAATACA GCGGCAGCCG GACACCGGTA 1201 TATGTCGATG TAT TGAGCT GGACGAAAAA GGCAACAAGA TTCAGGAGAC 1251 CAATCCCGAC GGCACGCCTG CCTTTACCGG TTTTTCCGGT ACGGTGCCGG 1301 TTTGGAAAAC CGTCAAAGTG GCAGACGACC ATGTTCCTGC GCTGTATAAC 1351 TACGCCAAAT ACCTCAACAC CAACAAAACC CATTCGCTGA CTGCCAGCAC 1401 GCGTTTCAAC GTAACCGGCC GACTGCACCT TTTGGGCGGG CTGCACTACA 1451 CGCGCTATGA GACTTCGCAA ACCAAAGATA TGCCTGTCCG CTATGGGCAG 1501 CCGGCAAGCG ATTTTCAGAC GGCATCGAGC ATTAGGGCGG ATCAGGACCA 1551 TTACACGGCC AAGATGCAAG GTCATAAATT GACGCCCTAT GCAGGCATTA 1601 CCTATGACTT GACACCGCAA CAGAGTATTT ACGGAAGTTA TACCAAAATC 1651 TTCAAACAGC AGGATAATGT CGATGTCAGT GCCAAAACCG TTTTACCGCC 1701 TTTGGTCGGC ACAAACTATG AGGTAGGCTG GAAAGGCGCG TTCTTGCAAG 1751 GACGGCTGAA TGCTTCGTTC GCATTGTTTT ACCTCGAACA GAAAAACCGC 1801 ACGGTCGTCG ATTTCGGCTA TGTTCCCGGA GCAGGCGGCA AGCAGGGGTC 1851 GTTCCAAACC GTTGCCAAAC CGATAGGCAA AGTGGTCAGC AGGGGTGCGG 1901 AATTCGAGTT GTCGGGTGAG TTGAACGAAG ATTGGAAAGT CTTTGCGGGT 1951 TACACCTACA ACAAGAGCCG CTACAAAAAC GCCGCCGAAG TCAACGCCGA 2001 ACGCCTTGCC AAAAATTCCA GTGCAGACCC GTACAACTTC AGCAATTTCA 2051 CACCCGTGCA CATATTCCGT TTCGGAACGA GCTTCCATAT ACCGAATACG 2101 GGGCTGACCG TCGGCGGCGG CGTGTCCGCA CAAAGCGGCA CAAGCAGTCT 2151 GTATAACATC AGGCAGGGCG GCTACGGGCT GATAGACGGT TTCGTCCGTT 2201 ACGAATTGGG CAAACACGCC AAATTGAGCC TCATCGGTAC GAACTTAAAC 2251 GGACGCACTT ATTTTGAGAA CAACTACAAC CGTACGCGCG GCGCAAACAA 2301 CTTCTACGGA GAGCCGCGCA CTGTCAGCAT GAAACTGGAT TGGCAGTTTT 2351 AA This corresponds to the amino acid sequence <SEQ ID 2540; ORF 742>: m742.pep 1 MVYGIAEADA GDSSVLTLGG MYQKSREVPD FSGIILPCEN QKTAPFSSTP 51 ACNRPLQLPR NTYLGEDWSR LSADKYNLFS GFKHVFDNGW QLNAEVSYTK 101 NESDAKVGQF FLKNEYAAGL SGEDAVGFLT EKNEVIPFEP KDKALEKLKA 151 YRDETAKEYR ERKDDFVKNR FDNTAFEQYR SRRAAERKAG FDKCMSDPFA 201 LDFICQGSWG DPGVDADKAE FVDKALAKEG IFNNAAQRFP NSLYDSSFNR 251 KATANRRYSY MPLRHTKDDR QWGIKLDLTG TYGLFGREHD FFVGYAYGDE 301 KIRSEYLEIY ERRYRVRPNT GATHGVYAGS CQEEPDGDLS SPLVRGHKEP 351 DWQAYDEKGN RTVYAEECRN AKKIKTEPKL DAEGKQVYYY DEYSGSRTPV 401 YVDVYELDEK GNKIQETNPD GTPAFTGFSG TVPVWKTVKV ADDHVPALYN 451 YAKYLNTNKT HSLTASTRFN VTGRLHLLGG LHYTRYETSQ TKDMPVRYGQ 501 PASDFQTASS IRADQDHYTA KMQGHKLTPY AGITYDLTPQ QSIYGSYTKI 551 FKQQDNVDVS AKTVLPPLVG TNYEVGWKGA FLQGRLNASF ALFYLEQKNR 601 TWDFGYVPG AGGKQGSFQT VAKPIGK S RGAEFELSGE LNEDWKVFAG 651 YTYNKSRYKN AAEVNAERLA KNSSADPYNF SNFTPVHIFR FGTSFHIPNT 701 GLTVGGGVSA QSGTSSLYNI RQGGYGLIDG FVRYELGKHA KLSLIGTNLN 751 GRTYFENNYN RTRGANNFYG EPRTVSMKLD WQF* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2541 >: a742.seq 1 ATGGTTTACG GCATTGCCGA AGCCGATGCG GGCGACAGCA GTGTGCTTAC 51 TTTGGGCGGC ATGTATCAGA AGAGTAGGGA GGTTCCTGAT TTTTCGGGCA 101 TTATTTTGTC CTGTGAAAAT CAGAAAACTG CCCCGTTCAG TTCAACGCCT 151 GCCTGCAACC GGCCTTTGCA ACTGCCGCGC AACACTTATT TGGGGGAGGA 201 TTGGTCGCGG TTGAGTGCTG ACAAATACAA CCTTTTCTCA GGTTTCAAAC 251 ATGTGTTTGA CAACGGTTGG CAGCTCAATG CCGAAGTGTC TTATACCAAG 301 AATGAATCCG ATGCGAAGGT GGGGCAGTTT TTTCTGAAAA ACGAACATGC 351 GGCGGGTTTG TCAGATGAGG ATGCGGTAGG CTTTTTGACC GAAAAAAACG 401 AAGTCATCCC GTTCGAGCCG AAAGATAAGG CATTGGAGAA ACTGAAAGCA 451 TATCGTGACG AAACCGCCAA GGAATACCGT GAGCGCAAAG ACGATTTTGT 501 TAAAAACCGT TTCGATAATA CTGCTTTCGA GCAGTACCGC AGCCGCCGTG 551 CCGCAGAACG CAAAGCCGGT TTTGACGAGT GTATGAGTGC CCCTTTTGCG 601 CTGGACTTTA TCTGTCAAGG TTCTTGGGGG GATCCGGGTG TTGATGCCGA 651 CAAGTCGGAA TTTGTCGATA AAGCCCTTGC GAAGGAAGGC ATCTTTAATA 701 ATGCGGCACA ACGTTTTCCA AACAGCCTGT ATGACTCTTC CTTTAATCGG 751 AAGGCTACCG CCAACCGACG ATACAGTTAT ATGCCGTTGC GGCATACCAA 801 AGACGACCGC CAATGGGGAA TTAAACTTGA CCTGACCGGC ACATATGGGC 851 TGTTCGGGCG GGAGCATGAT TTCTTTGTCG GCTATGCCTA CGGCGATGAA 901 AAGATACGTT CCGAATATCT GGAAATCTAC GAACGCCGCC ACAGAGTACG 951 TCCGAATACA GGGGCAACGC ACGGCGTGTA TGCGGGAAGT TGTCAGGGGG 1001 AGCCGGACGG TGATTTGTCT TCTCCTTTGG TCAGGGGGCA TAAAGAACCC 1051 GATTGGCAGG CGTACGATGA AAAAGGCAAC CGTACCGTTT ATGCCGAAGA 1101 ATGCAGGAAT GCCAAGAAAA TAAAAACCGA GCCCAAGCTC GATGCCGAAG 1151 GCAAGCAGGT GTATTACTAT GACGAATACA GCGGCAGCCG GACGCCAGTA 1201 TATGTCGATG TATATGAACT GGATGAAAAA GGCAATAAGA TTCAGGAGAC 1251 CAATCCCGAC GGCACGCCTG CCTTTACCGG TTTTTCCGGT ACGGTGCCGG 1301 TTTGGAAAAC CGTCAAAGTG GCCGACGACC ATGTTCCTGC GCTGTATAAC 1351 TACGCCAAAT ACCTCAACAC CAACAAAACC CATTCGCTGA CTGCCGGCAC 1401 GCGTTTCAAC GTAACCGGCC GACTGCATCT TTTGGGCGGG CTGCACTACA 1451 CGCGCTATGA AACCTCGCAA ACCAAAGATA TGCCTGTCCG CTATGGGCAG 1501 CCGGCAAGCG ATTTTCAGAC GGCATCGAGC ATTAAGGCGG ATCAGGACCA 1551 TTATACGGCC AAGATGCAAG GTCATAAATT GACGCCCTAT GCAGGCATTA 1601 CCTATGATTT GACACCGCAA CAGAGTATTT ACGGAAGTTA TACCAAAATC 1651 TTCAAACAGC AGGATAATGT CGATGTCAGT GCCAAAACCG TTTTACCGCC 1701 TTTGGTCGGC ACAAACTATG AGGTAGGCTG GAAAGGCGCG TTCTTGCAAG 1751 GACGGCTGAA TGCTTCGTTC GCATTGTTTT ACCTCGAACA GAAAAACCGC 1801 ACGGTCGTCG ATTTTGGCTA TGTTCCCGGA GCAGGCGGCA AGCAGGGGTC 1851 GTTCCAAACC GTTGCCAAAC CGATAGGCAA AGTGGTCAGC AGGGGTGCGG 1901 AATTCGAGTT GTCGGGTGAG TTGAACGAAG ATTGGAAAGT CTTTGCGGGT 1951 TACACCTACA ACAAGAGCCG CTACAAAAAC GCCGCCGAAG TCAACGCCGA 2001 ACGCCTCGCC AAAAACACAG CCGCAGACCC GTACAACTTC AGCAATTTCA 2051 CACCCGTGCA CATATTCCGT TTCGGAACGA GCTTCCATAT ACCGAATACG 2101 GGGCTGACCG TCGGCGGCGG CGTGTCCGCA CAAAGCGGCA CAAGCAGTCT 2151 GTATAACATC AGGCAGGGCG GCTACGGGCT GATAGACGGT TTCGTCCGTT 2201 ACGAATTGGG CAAACACGCT AAATTGAGCC TCATCGGTAC GAACTTAAAC 2251 GGACGCACTT ATTTTGAGAA CAACTACAAC CGTACGCGCG GCGCAAACAA 2301 CTTCTATGGA GAGCCGCGCA CTGTCAGCAT GAAACTGGAT TGGCAGTTTT 2351 AA This corresponds to the amino acid sequence <SEQ ID 2542; ORF 742.a>: a742.peρ 1 MVYGIAEADA GDSSVLTLGG MYQKSREVPD FSGIILSCEN QKTAPFSSTP 51 ACNRPLQLPR NTYLGEDWSR LSADKYNLFS GFKHVFDNGW QLNAEVSYTK 101 NESDAKVGQF FLKNEHAAGL SDEDAVGFLT EKNEVIPFEP KDKALEKLKA 151 YRDETAKEYR ERKDDFVKNR FDNTAFEQYR SRRAAERKAG FDECMSAPFA 201 LDFICQGSWG DPGVDADKSE FVDKALAKEG IFNNAAQRFP NSLYDSSFNR 251 KATANRRYSY MPLRHTKDDR QWGIKLDLTG TYGLFGREHD FFVGYAYGDE 301 KIRSEYLEIY ERRHRVRPNT GATHGVYAGS CQGEPDGDLS SPLVRGHKEP 351 DWQAYDEKGN RTVYAEECRN AKKIKTEPKL DAEGKQVYYY DEYSGSRTPV 401 YVDVYELDEK GNKIQETNPD GTPAFTGFSG TVPVWKTVKV ADDHVPALYN 451 YAKYLNTNKT HSLTAGTRFN VTGRLHLLGG LHYTRYETSQ TKDMPVRYGQ 501 PASDFQTASS IKADQDHYTA KMQGHKLTPY AGITYDLTPQ QSIYGSYTKI 551 FKQQDNVDVS AKTVLPPLVG TNYEVGWKGA FLQGRLNASF ALFYLEQKNR 601 TWDFGYVPG AGGKQGSFQT VAKPIGKWS RGAEFELSGE LNEDWKVFAG 651 YTYNKSRYKN AAEVNAERLA KNTGADPYNF SNFTPVHIFR FGTSFHIPNT 701 GLTVGGGVSA QSGTSSLYNI RQGGYGLIDG FVRYELGKHA KLSLIGTNLN 751 GRTYFENNYN RTRGANNFYG EPRTVSMKLD WQF* a742/m742 98.5% identity in 783 aa overlap 10 20 30 40 50 60 a742.pep MVYGIAEADAGDSSVLTLGGMYQKSREVPDFSGIILSCENQKTAPFSSTPACNRPLQLPR m742 MVYGIAEADAGDSSVLTLGGMYQKSREVPDFSGIILPCENQKTAPFSSTPACNRPLQLPR 10 20 30 40 50 60 70 80 90 100 110 120 a742.pep NTYLGEDWSRLSADKYNLFSGFKHVFDNGWQLNAEVSYTKNESDAKVGQFFLKNEHAAGL I II II II II II II II I II I II II I I II I II II II II I II I II II I II II II II II : I I II m742 NTYLGEDWSRLSADKYNLFSGFKHVFDNGWQLNAEVSYTKNESDAKVGQFFLKNEYAAGL 70 80 90 100 110 120 130 140 150 160 170 180 a742.pep SDEDAVGFLTEKNEVIPFEPKDKALEKLKAYRDETAKEYRERKDDFVKNRFDNTAFEQYR I II II II II II II II II II I II II II II II I II II II I II II II II II II I II II II II m742 SGEDAVGFLTEKNEVIPFEPKDKALEKLKAYRDETAKEYRERKDDFVKNRFDNTAFEQYR 130 140 150 160 170 180 190 200 210 220 230 240 a742.pep SRRAAERKAGFDECMSAPFALDFICQGSWGDPGVDADKSEFVDKALAKEGIFNNAAQRFP I I I II I II II II : I II II II II I I II II I II II II I I : I II I II I I II I II II II II I I m742 SRRAAERKAGFDKCMSDPFALDFICQGSWGDPGVDADKAEFVDKALAKEGIFNNAAQRFP 190 200 210 220 230 240 250 260 270 280 290 300 a742.pep NSLYDSSFNRKATANRRYSYMPLRHTKDDRQWGIKLDLTGTYGLFGREHDFFVGYAYGDE II I II I II II I II I I II I II II II II I I I II I II I I I I I II I II I I I I I I I I II II II II m742 NSLYDSSFNRKATANRRYSYMPLRHTKDDRQWGIKLDLTGTYGLFGREHDFFVGYAYGDE 250 260 270 280 290 300 310 320 330 340 350 360 a742.pep KIRSEYLEIYERRHRVRPNTGATHGVYAGSCQGEPDGDLSSPLVRGHKEPDWQAYDEKGN I II II I I II II I I : II I II I II I I I II I II II I I II II II I II II I I I I II I I I II II I 742 KIRSEYLEIYERRYRVRPNTGATHGVYAGSCQEEPDGDLSSPLVRGHKEPDWQAYDEKGN 310 320 330 340 350 360 370 380 390 400 410 420 a742.pep RTVYAEECRNAKKIKTEPKLDAEGKQVYYYDEYSGSRTPVYVDVYELDEKGNKIQETNPD I I I II I II II II II II II II II II I II II II I II I II II II II II II I I I I II I II II II m742 RTVYAEECRNAKKIKTEPKLDAEGKQVYYYDEYSGSRTPVYVDVYELDEKGNKIQETNPD 370 380 390 400 410 420 430 440 450 460 470 480 a742. pep GTPAFTGFSGTVPVWKTVKVADDHVPALYNYAKYLNTNKTHSLTAGTRFNVTGRLHLLGG I II II I II II II II I II II II II II II II I II I II II I II II I IM I I I I II II II II I I m 42 GTPAFTGFSGTVPVWKTVKVADDHVPALYNYAKYLNTNKTHSLTASTRFNVTGRLHLLGG 430 440 450 460 470 480 490 500 510 520 530 540 a742. pep LHYTRYETSQTKDMPVRYGQPASDFQTASSIKADQDHYTAKMQGHKLTPYAGITYDLTPQ I II II I II I II II I I II II II II I II I II I I : II II I II II I II II II II II I II II I I I m742 LHYTRYETSQTKDMPVRYGQPAΞDFQTASSIRADQDHYTAKMQGHKLTPYAGITYDLTPQ 490 500 510 520 530 540 550 560 570 580 590 600 a742.pep QSIYGSYTKIFKQQDNVDVSAKTVLPPLVGTNYEVGWKGAFLQGRLNASFALFYLEQKNR II I II I II II I II I II II II II I II II II II II II II II II II I II II I I II I II II I II m742 QSIYGSYTKIFKQQDNVDVSAKTVLPPLVGTNYEVGWKGAFLQGRLNASFALFYLEQKNR 550 560 570 580 590 600 610 620 630 640 650 660 a742.pep TVVDFGYVPGAGGKQGSFQTVAKPIGKVVSRGAEFELSGELNEDWKVFAGYTYNKSRYKN I II I II II I I II II II I II II II II I II II I II II I I I I II I II II I I I I I I I II I II II m742 TWDFGYVPGAGGKQGSFQTVAKPIGKVVSRGAEFELSGELNEDWKVFAGYTYNKSRYKN 610 620 630 640 650 660 670 680 690 700 710 720 a742.pep AAEVNAERLAKNTGADPYNFSNFTPVHIFRFGTSFHIPNTGLTVGGGVSAQSGTSSLYNI II I I II II II II :: II II II I I II I I II II II I II II II II II II I II II I II II II I II m742 AAEVNAERLAKNSSADPYNFSNFTPVHIFRFGTSFHIPNTGLTVGGGVΞAQSGTSSLYNI 670 680 690 700 710 720 730 740 750 760 770 780 a742.pep RQGGYGLIDGFVRYELGKHAKLSLIGTNLNGRTYFENNYNRTRGANNFYGEPRTVSMKLD I I II II II II II II II II II II II II I II II II II II I II II II II I I II II II II II I I m742 RQGGYGLIDGFVRYELGKHAKLSLIGTNLNGRTYFENNYNRTRGANNFYGEPRTVSMKLD 730 740 750 760 770 780 a742.pep WQFX II II m742 WQFX a742/ p25184 sp|P25184 |PUPA_PSEPU FERRIC-PSEUDOBACTIN 358 RECEPTOR PRECURSOR >gi|94923|pir| IS15169 ferric-pseudobactin receptor precursor - Pseudomonas putida >gi 145723 (X56605) pseudobactin uptake protein [Pseudomonas putida] Length = 819 Score = 152 bits (381), Expect = 6e-36 Identities = 110/356 (30%), Positives = 170/356 (46%), Gaps = 55/356 (15%) Query: 436 KTVKVADDHV-PALYNYAKYLNTNKTHSLTAGTRFNVTGRLHLLGGLHYTRYETSQTKDM 494 +T K DD + P + +Y +N+ +RFN+T LHL+ G + Y Sbjct: 511 QTPKPGDDEIIPGI QYNISNRQSGYFVASRFNLTDDLHLILGARASNYRFDYAL— 564 Query: 495 PVRYGQPASDFQTASSIKADQDHYTAKMQGHKLTPYAGITYDLTPQQSIYGSYTKIFKQQ 554 R G + ++ ++ +TPYAGI YDLT +QS+Y SYT IFK Q Sbjct: 565 -WRIGNEPAPYKM VERGVVTPYAGIVYDLTNEQSVYASYTDIFKPQ 609 Query: 555 DNVDVSAKTVLPPLVGTNYEVGWKGAFLQGRLNASFALFYLEQKNRTVVDFGYVPGAGGK 614 +NVD++ K L P VG NYE+GWKG FL+GRLNA+ AL+ +++ N VP +GG Sbjct: 610 NNVDITGKP-LDPEVGKNYELGWKGEFLEGRLNANIALYMVKRDNLAESTNEWPDSGGL 668 Query: 615 QGSFQTVAKPIGKWSRGAEFELSGELNEDWKVFAGYTYNKSRYKNAAEVNAERLAKNTG 674 S + + ++G + ELSGE+ W VF GY++ ++ Sbjct: 669 IAS RAVDGAETKGVDVELSGEVLPGWNVFTGYSHTRTE D 707 Query: 675 ADPYNFSNFTPVHIFRFGTSFHIPN—TGLTVGGGVSAQSGTS SLYN—IRQGGYGL 727 AD + P+ FRF ++ +P LT+GGGV+ S ++ + YN + Q Y + Sbjct: 708 ADGKRLTPQLPMDTFRFWNTYRLPGEWEKLTLGGGVNWNSKSTLNFARYNSHVTQDDYFV 767 Query: 728 IDGFVRYELGKHAKLSLIGTNLNGRTYFENNYNRTRGANNFYGEPRTVSMKLDWQF 783 RY + + +L N+ + Y Y G+ YG PR ++ L + F Sbjct: 768 TSLMARYRINESLAATLNVNNIFDKKY YAGMAGSYGHYGAPRNATVTLRYDF 819 g743.seq not found yet g743.pep not found yet The following partial DNA sequence was identified in N meningitidis <SEQ ID 2543>: m743. seq 1 ATGAATCAAA ATCATTTTTC ACTTAAAATT CTGACCGTTA TGCTGTTATC 51 GGCTTACGGT GGTTCTTTTG CAGACGGTGT TGTGCCTGTT TCAGACGGCA 101 ATACCGTCAG TCTGGATACG GTCAATGTAC GCGGCTCTCA TGCTTTGTTG 151 GGCAAGACCG AAAAGACCCG TTCTTATACG ATAGATCGGA TGTCCACCGC 201 CACAGGTATG AGGATTGCGG GCAAGGATAC GCCGCAGTCG GTCAGCGTCA 251 TCACGCGCAG CCGCCTTGAC GATAAGGCGG TGCATACGCT TGAAGAGGCA 301 ATGAAAAACA CGACGGGTGT CAACGTTGTG CGCGATTCAG GCTTGCAGAC 351 GCGGTTTTTG TCACGCGGTT TCTATATTGA TCAGATTGGT GAAGACGGTA 401 TGACCGTCAA TGTTGCAGGC CGTTCGGGAT ATACGGCGAA AATCGACGTG 451 TCTCCGAGTA CCGATTTGGC GGTTTATGAC CATATTGAAG TTGTACGGGG 501 TGCAACGGGG TTGACCCAAT CCAATTCAGA GCCGGGAGGA ACCGTCAATT 551 TGATCCGTAA GTGA This corresponds to the amino acid sequence <SEQ ID 2544; ORF 743>: m7 3.pep 1 MΝQΝHFSLKI LTVMLLSAYG GSFADGWPV SDGΝTVSLDT VΝVRGSHALL 51 GKTEKTRSYT IDRMSTATGM RIAGKDTPQS VSVITRSRLD DKAVHTLEEA 101 MKΝTTGVΝW RDSGLQTRFL SRGFYIDQIG EDGMTVΝVAG RSGYTAKIDV 151 SPSTDLAVYD HIEWRGATG LTQSΝSEPGG TVΝLIRK* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2545>: a743. seq 1 ATGAATCAAA ATCATTTTTC ACTTAAAATT CTGACCGTTA TGCTGTTATC 51 GGCTTACGGT GGTTCTTTTG CAGACGGTGT TGTGCCTGTT TCAGACGGCA 101 ATACCGTCAG TTTGGATACG GTCAATGTAC GCGGCTCTCA TGCTCTGTCG 151 GGCAAGACCG AGAAGACCCG TTCTTATACG ATAGATCGGA TGTCCACCGC 201 CACAGGTATG AGGATTGCGG GCAAGGATAC GCCGCAGTCG GTCAGCGTCA 251 TCACGCGCAG CCGCCTTGAC GATAAGGCGG TGCATACGCT TGAAGAGGCA 301 ATGAAAAACA CGACGGGTGT CAACGTTGTG CGCGATTCAG GCTTGCAGAC 351 GCGGTTTTTG TCACGCGGTT TCTATATTGA TCAGATTGGT GAAGACGGTA 401 TTACCGTCAA TGTTGCAGGC CGTTCGGGAT ATACGGCGAA AATCGACGTG 451 TCTCCGAGTA CCGATTTGGC GGTTTATGAC CATATTGAAG TTGTACGGGG 501 TGCAACGGGG TTGACCCAAT CCAATTCAGA GCCGGGTGGA ACCGTCAATT 551 TGATCCGTAA GCGA This corresponds to the amino acid sequence <SEQ ID 2546; ORF 743. a>: a7 3.pep 1 MNQNHFSLKI LTVMLLSAYG GSFADGWPV SDGNTVSLDT VNVRGSHALS 51 GKTEKTRSYT IDRMSTATGM RIAGKDTPQS VSVITRSRLD DKAVHTLEEA 101 MKNTTGVNW RDSGLQTRFL SRGFYIDQIG EDGITVNVAG RSGYTAKIDV 151 SPSTDLAVYD HIEWRGATG LTQSNSEPGG TVNLIRKR a743/m743 98.9% identity in 187 aa overlap 10 20 30 40 50 60 a7 3.pep MNQNHFSLKILTVMLLSAYGGSFADGWPVSDGNTVSLDTVNVRGSHALSGKTEKTRSYT II II I II II II II II I II II I II II I II I II II II I II II I II II I II I II II II II II m743 MNQNHFSLKILTVMLLSAYGGSFADGWPVSDGNTVSLDTVNVRGSHALLGKTEKTRSYT 10 20 30 40 50 60 70 80 90 100 110 120 a743.pep IDRMSTATGMRIAGKDTPQSVSVITRSRLDDKAVHTLEEAMKNTTGVNWRDSGLQTRFL MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM m743 IDRMSTATGMRIAGKDTPQSVSVITRSRLDDKAVHTLEEAMKNTTGVNWRDSGLQTRFL 70 80 90 100 110 120 130 140 150 160 170 180 a743.pep SRGFYIDQIGEDGITVNVAGRSGYTAKIDVSPSTDLAVYDHIEWRGATGLTQSNSEPGG I I II II II I II II M I II I II II II II I I II II II I II II I II II II I I II I II II II I I m743 SRGFYIDQIGEDGMTVNVAGRSGYTAKIDVSPSTDLAVYDHIEWRGATGLTQSNSEPGG 130 140 150 160 170 180 a743.pep TVNLIRKR lllllll m743 TVNLIRKX g744.sβq not found yet g744.pep not found yet The following partial DNA sequence was identified in N meningitidis <SEQ ID 2547>: m744.seq 1 ATGAAACCGT TAAAAACATT AGAATTTGGA TTTGTGGATG CTGCAAACTA 51 CAGAAGAAGA GAAAATAAAG ATTTATTTAA CCGAATATTT GTAAAAGGAG 101 AATATTTGGA TGAATTATGT GAACCAAATA TTTCGTTTTT AATCGGAGAA 151 AAGGGAACTG GAAAGACAGC ATATGCTGTT TATTTAACTA ATAACTTCTA 201 TAAAAACATA CATGCCACTA CTAAGTTTGT TCGTGAAACC GATTATTCAA 251 AATTTATTCA GCTAAAGAAA GCAAGACACT TAACTGTTTC AGATTTTACA 301 AGTATTTGGA AAGTCATTTT ATATCTGTTG ATATCAAATC AAATCAAATG 351 TAAAGAAAAC GGAATATTAT CTTCAATATT TAATAAATTT AAAGCCTTAG 401 ATGAGGCTAT AAATGAATAT TATTATGGCG CTTTTGATCC GGAAATTGTA 451 CAAGCAATAA CTTTAATAGA AAATTCAAAA GAAGCTGCGG AAATGATTTT 501 TGGAAAATTT GTTAAACTAG GTGAAGAGGA ATCCCAACAA ATAACTTTTA 551 CAGAAAGTAA ATTCCAAGCA AATTTAGGTT TTATTGAAAG AAAATTTAAA 601 GATGCTTTAT CTCAGTTAAA GCTAAAAGAT AATCATATTT TGTTTATTGA 651 TGGGATAGAT ATTAGACCAT CACAGATTCC ATTTGATGAA TATCATGAGT 701 GTGTAAAAGG TCTTGCTAAC GCCATATGGA TGTTAAATAA TGATATCTTC 751 CCTTCCATTA AAGATAGTAA GGGAAGGATG AGAGTTGTGT TATTGATTAG 801 ACCTGATATC TTTGATTCAT TAGGTTTACA AAATCAAAAT ACCAAACTTC 851 AAGATAATTC AGTATTTTTA GACTGGAGGA CGGATTATAA ATCTTATAGA 901 AGTTCAAAGA TTTTTGGCGT TTTTGATCAT CTTTTGAGAA CCCAGCAAGA 951 AAAACAAGAT AGTTTAGAAA AAGGCAACTC ATGGGATTAT TATTTTCCAT 1001 GGAATGCTCC TAATTTACAT GATGAGTATA AAAATTTAAC TTCATTTATT 1051 AGCTTCCTAA GAAAATCGTA TTATCGACCT CGCGATATTC TTCAGATGCT 1101 TACTTTGCTA CAAAAAAATA AGAAAAGTAA GGAAGATTAT GTCGTAGCAG 1151 AAGATTTTGA TAATACTTCT TTTCAAAGAG AATACTCGAT ATATTTACTT 1201 GGTGAAATCA AAGATCATCT TTTGTTTTAT TATAGTCAAA GTGATTATCA 1251 AAATTTCCTG AAATTTTTTG AATTTTTAAA CGGGAAAGAT AGATTTAAAT 1301 ATAGTGATTT TTTAAAAGCA TTTGAACGTT TGAAAAAGCA CTTACAAACA 1351 ACATCAGTGG AAATACCTAA ATTTATGAGT ACTGCTAATG AGTTTTTGCA 1401 ATTTTTATTT GACTTGAATG TTATTGCTTA TTTAGATAAC CCAGAAGATG 1451 AAACGAAACC ATATATCCAT TGGTGCTTTA AAGATAGAAA TTATGCAAAT 1501 ATTTCTCCTA AAATAAAAAC TGAAACTGAA TATTTAATAT TTTCAGGATT 1551 ATCAAAAGCC CTTGATGTTG GTACTCCATT TAAGAACAAA CAGTAA This corresponds to the amino acid sequence <SEQ ID 2548; ORF 744>: m744.pep 1 MKPLKTLEFG FVDAANYRRR ENKDLFNRIF VKGEYLDELC EPNISFLIGE 51 KGTGKTAYAV YLTNNFYKNI HATTKFVRET DYSKFIQLKK ARHLTVSDFT 101 SIWKVILYLL ISNQIKCKEN GILΞSIFNKF KALDEAINEY YYGAFDPEIV 151 QAITLIENSK EAAEMIFGKF VKLGEEEΞQQ ITFTESKFQA NLGFIERKFK 201 DALΞQLKLKD NHILFIDGID IRPSQIPFDE YHECVKGLAN AIWMLNNDIF 251 PSIKDSKGRM RVVLLIRPDI FDSLGLQNQN TKLQDNSVFL DWRTDYKSYR 301 SΞKIFGVFDH LLRTQQEKQD ΞLEKGNSWDY YFPWNAPNLH DEYKNLTΞFI 351 SFLRKSYYRP RDILQMLTLL QKNKKSKEDY VVAEDFDNTS FQREYSIYLL 401 GEIKDHLLFY YSQSDYQNFL KFFEFLNGKD RFKYSDFLKA FERLKKHLQT 451 TSVEIPKFMS TANEFLQFLF DLNVIAYLDN PEDETKPYIH WCFKDRNYAN 501 ISPKIKTETE YLIFΞGLSKA LDVGTPFKNK Q* g7 5.seq not found yet g745.pep not found yet The following partial DNA sequence was identified in N meningitidis <SEQ LD 2549>: m745. seq 1 ATGTTTTGGC AACTGACCGT TGTTTCAGTA ACCGCCGTCA TTGCACTGGG 51 GACAATATTC ATCAATAAGA AAACTTCAAA GCAAAAGGCG ACATTAGATG 101 TTATTTTGAA TGATTACCAA GATGCACAAT TTGTAGAAGC CGACAATCAT 151 ATTTCGCCTT ATATTCGCGG CACGGCAGTT GACGACAACA ACGCGCGGAT 201 CGACCTGTAT GAAATTTATC AAAATAAGGG CGGACAATGG GAAAAAGAGA 251 GAGGGCATTT ACTTACCGTA ATCAATCGGC ACGAGTTTTA TGCGTGCGCA 301 ATCAACTCGG GAGTATTGGA TGAGGATTTG TTTAAACGGC TGCATTGCAC 351 CAACTTCATA AAATTGTGGA ATGCAGTTTC GCCTCTTGTT ATGAAAATAC 401 GCGAAGAAGA ACGCAAAGAC ACAATATTTA GAGAGTTGGA AATTTTGGTT 451 GCATTATGGA AAGCAAACCC CCTAAAGGCA TCTGATTTGT GA This corresponds to the amino acid sequence <SEQ LD 2550; ORF 745>: m745.pep 1 MFWQLTWSV TAVIALGTIF IΝKKTSKQKA TLDVILΝDYQ DAQFVEADΝH 51 ISPYIRGTAV DDΝΝARIDLY EIYQΝKGGQW EKERGHLLTV IΝRHEFYACA 101 IΝSGVLDEDL FKRLHCTΝFI KLWΝAVSPLV MKIREEERKD TIFRELEILV 151 ALWKAΝPLKA SDL* a745.seq not found yet a745.pep not found yet The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 2551>: g746.seq 1 ATGTCCGAAA ACAAACAAAA CGAAGTCCTG ACCGGTTACG AACAGCTGAA 51 ACGGCGCAAC CGCCGCCGCC TCGTAACGGC AAGCTCCCTG GTTGCCGCCT 101 CCTGCATCCT GCTGGCAGCC GCACTCAGTT CCGATCCTGC CGACAGCAAT 151 CCCGCACCGC AGGCCGGCGA AACCGGCGCA ACGGAAAGCC AAACGGCAAA 201 CACGGCACAA ACCCCTGCCT TGAAATCCGC CGCCGAAAAC GGGGAAACCG 251 CCGCCGACAA ACCGCAGGAC TTGGCAGGCG AAGACAAGCC TTCTGCCGCC 301 GACAGCGAAA TCAGCGAGCC TGAAAACGTA GGCGCGCCGC TGGTGCTGAT 351 TAACGACCGG CTCGAAGACA GCAACATCAA AGGTTTGGAA GAATCCGAGA 401 AACTGCAACA GGCAGAAACC GCCAAAACCG AACCGAAGCA GGCAAAACAA 451 CGCGCTGCCG AAAAAGTGTC GGCAACTGCC GACAGTACGG ATACGGTAGC 501 GGTTGAAAAA CCGAAACGCA CTGCCGAACC CAAACCGCAA AAAGCGGAAC 551 GCACTGCCGA AGCCAAGCCC AAAGCCAAAG AAACCAAAAC CGCCGAAAAA 601 GTTGCCGACA AACCGAAAAC TGCTGCCGAA AAAACCAAAC CGGATACGGC 651 AAAATCCGAC AGCGCGGTAA AAGAAGCGAA AAAAGCCGAC AAGGCTGAAG 701 GCAAAAAGAC AGCCGAAAAA GACCGTTCGG ACGGCAAAAA ACACGAAACG 751 GCGCAAAAAA CCGACAAAGC GGACAAAACC AAAACCGCCG AGAAGGAAAA 801 ATCCGGCAAG GCGGGCAAAA AAGCCGCCAT TCAGGCAGGT TATGCCGAAA 851 AAGAACGCGC CTTGAGCCTC CAGCGCAAAA TGAAGGCGGC GGGTATCGAT 901 TCGACCATCA CCGAAATCAT GACCGACAAC GGCAAAGTTT ACCGCGTCAA 951 ATCAAGCAAC TATAAAAACG CAAGGGATGC CGAACGCGAT TTGAACAAAC 1001 TGCGCGTGCA CGGCATCGCC GGCCAGGTAA CGAATGAATA G This corresponds to the amino acid sequence <SEQ ID 2552; ORF 746.ng>: g746.pep 1 MSENKQNEVL TGYEQLKRRN RRRLVTASSL VAAΞCILLAA ALΞΞDPADΞN 51 PAPQAGETGA TESQTANTAQ TPALKΞAAEN GETAADKPQD LAGEDKPSAA 101 DΞEIΞEPENV GAPLVLINDR LEDSNIKGLE ESEKLQQAET AKTEPKQAKQ 151 RAAEKVSATA DSTDTVAVEK PKRTAEPKPQ KAERTAEAKP KAKETKTAEK 201 VADKPKTAAE KTKPDTAKSD SAVKEAKKAD KAEGKKTAEK DRSDGKKHET 251 AQKTDKADKT KTAEKEKΞGK AGKKAAIQAG YAEKERALSL QRKMKAAGID 301 STITEIMTDN GKVYRVKSSN YKNARDAERD LNKLRVHGIA GQVTNE* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2553>: m746.seq 1 ATGTCCGAAA ACAAACAAAA CGAAGTCCTG AGCGGTTACG AACAACTCAA 51 ACGGCGCAAC CGCCGCCGCC TCGTAACGGC AAGTTGCCTG GTTGCCGCCT 101 CCTGCATCCT GCTGGCAGCC GCCCTCAGTT CCGGCCCTGC CGAACAGACT 151 GCCGGCGAAA CAAGCGGCGT AGAAAACAAA GCGGCAGGTG CGGCACAAAC 201 CCCTGCCTTG AAATCCGCCG CCGACAAACC GCAGGACTTG GCAGGCGAAG 251 ACAAGCCTTC TGCCGCCGAC AGCGAAATCA GCGAGCCTGA AAACGTAGGC 301 GCGCCGCTGG TGCTGATTAA CGAGCGCCTC GAAGACAGCA ACATCAAAGG 351 TTTGGAAGCA TCCGAGAAAC TGCAACAGGC AGAAACCGCC AAAACCGCAC 401 CGAAGCAGGC AAAACAACGC GCTGCCGAAA AAGTGCCGGC AACTGCCGAC 451 AGTACGGATA CGGTAGCGGT TGAAAAACCG AAACGCACTG CCGAAACAAA 501 ACCGCAAAAA GCGGAACGCA CTGCCAAAGC CAAGCCCAAA GCCAAAGAAA 551 CCAAAACCGC CGAAAAAGTT GCCGACAAAC CGAAAACTGC CGCCGAAAAA 601 ACCAAACCGG ATACGGCAAA ATCCGACAGC GCGGTAAAAG AAGCGAAAAA 651 AGCCGACAAG GCTGAAAGCA AAAAAACAGC CGAAAAAGAC CGTTCGGACG 701 GCAAAAAACA CGAAACGGCA CAAAAAACCG ACAAAGCGGA CAAGACCAAA 751 ACCGCCGAGA AGGAAAAATC CGGTAAAAAA GCCGCCATTC AGGCAGGTTA 801 TGCCGAAAAA GAACGCGCCT TAAGCCTCCA GCGCAAAATG AAGGCGGCGG 851 GTATCGATTC GACCATCACC GAAATTATGA CCGACAACGG CAAAGTTTAC 901 CGCGTCAAAT CAAGCAACTA TAAAAACGCA AGGGATGCCG AACGCGATTT 951 GAACAAATTG CGCGTACACG GTATCGCCGG TCAGGTAACG AATGAATAG This corresponds to the amino acid sequence <SEQ ID 2554; ORF 746>: 1 MΞEΝKQΝEVL SGYEQLKRRΝ RRRLVTASCL VAAΞCILLAA ALSΞGPAEQT 51 AGETSGVEΝK AAGAAQTPAL KSAADKPQDL AGEDKPΞAAD SEISEPEΝVG 101 APLVLIΝERL EDSΝIKGLEA SEKLQQAETA KTAPKQAKQR AAEKVPATAD 151 STDTVAVEKP KRTAETKPQK AERTAKAKPK AKETKTAEKV ADKPKTAAEK 201 TKPDTAKSDS AVKEAKKADK AESKKTAEKD RSDGKKHETA QKTDKADKTK 251 TAEKEKΞGKK AAIQAGYAEK ERALSLQRKM KAAGIDSTIT EI TDΝGKVY 301 RVKΞΞΝYKΝA RDAERDLΝKL RVHGIAGQVT ΝE* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 746 shows 89.9% identity over a 346 aa overlap with a predicted ORF (ORF 746) from N gonorrhoeae: m746/g746 89.9% identity in 346 aa overlap 10 20 30 40 50 m746.pep MSEΝKQΝEVLSGYEQLKRRΝRRRLVTASCLVAASCILLAAALSSGPAEQT AGETSG I I II I I I I I M I I I I I I II I I I I II I I I I :: IMM: g746 MSEΝKQΝEVLTGYEQLKRRΝRRRLVTASSLVAASCILLAAALSSDPADSΝPAPQAGETGA 10 20 30 40 50 60 60 70 80 90 100 109 VEΝKAAGAAQTPALKSAA DKPQDLAGEDKPΞAADSEISEPEΝVGAPLVLIΝER :|::M :: I I I II I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M I g746 TESQTAΝTAQTPALKSAAEΝGETAADKPQDLAGEDKPΞAADSEISEPEΝVGAPLVLIΝDR 70 80 90 100 110 120 110 120 130 140 150 160 169 m746. pep LEDSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADΞTDTVAVEKPKRTAETKPQ llllllllll I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I III g746 LEDSNIKGLEESEKLQQAETAKTEPKQAKQRAAEKVSATADSTDTVAVEKPKRTAEPKPQ 130 140 150 160 170 180 170 180 190 200 210 220 229 m746. pep KAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKΞDSAVKEAKKADKAESKKTAEK I I I II I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I g746 KAERTAEAKPKAKETKTAEKVADKPKTAAEKTKPDTAKΞDSAVKEAKKADKAEGKKTAEK 190 200 210 220 230 240 230 240 250 260 270 280 m746.pep DRΞDGKKHETAQKTDKADKTKTAEKEKΞGK KAAIQAGYAEKERALSLQRKMKAAGID I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g746 DRSDGKKHETAQKTDKADKTKTAEKEKSGKAGKKAAIQAGYAEKERALSLQRKMKAAGID 250 260 270 280 290 300 290 300 310 320 330 m746.pep STITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNEX I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g74θ STITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNEX 310 320 330 340 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2555>: a746.seq 1 ATGTCCGAAA ACAAACAAAA CGAAGTCCTG AGCGGTTACG AACAACTCAA 51 ACGGCGCAAC CGCCGCCGCC TCGTAACGGC AAGTTGCCTG GTTGCCGCCT 101 CCTGCATCCT GCTGGCAGCC GCCCTCAGTT CCGGCCCTGC CGAACAGACT 151 GCCGGCGAAA CAAGCGGCGT AGAAAACAAA GCGGCAGGTG CGGCACAAAC 201 CCCTGCCTTG AAATCCGCCG CCGACAAACC GCAGGACTTG GCAGGCGAAG 251 ACAAGCCTTC TGCCGCCGAC AGCGAAATCA GCGAGCCTGA AAACGTAGGC 301 GCGCCGCTGG TGCTGATTAA CGACCGCCTC GAAGACAGCA ACATCAAAGG 351 TTTGGAAGCA TCCGAGAAAC TGCAACAGGC AGAAACCGCC AAAACCGCAC 401 CGAAGCAGGC AAAACAACGC GCTGCCGAAA AAGTGCCGGC AACTGCCGAC 451 AGTACGGATA CGGTAGCGGT TGAAAAACCG AAACGCACTG CCGAAACAAA 501 ACCGCAAAAA GCGGAACGCA CTGCCAAAGC CAAGCCCAAA GCCAAAGAAA 551 CCAAAACCGC CGAAAAAGTT GCCGACAAAC CGAAAACTGC CGCCGAAAAA 601 ACCAAACCGG ATACGGCAAA ATCCGACAGC GCGGTAAAAG AAGCGAAAAA 651 AGCCGACAAG GCTGAAAGCA AAAAAACAGC CGAAAAAGAC CGTTCGGACG 701 GCAAAAAACA CGAAACGGCA CAAAAAACCG ACAAAGCGGA CAAGACCAAA 751 ACCGCCGAGA AGGAAAAATC CGGTAAAAAA GCCGCCATTC AGGCAGGTTA 801 TGCCGAAAAA GAACGCGCCT TAAGCCTCCA GCGCAAAATG AAGGCGGCGG 851 GTATCGATTC GACCATCACC GAAATTATGA CCGACAACGG CAAAGTTTAC 901 CGCGTCAAAT CAAGCAACTA TAAAAACGCA AGGGATGCCG AACGCGATTT 951 GAACAAATTG CGCGTACACG GTATCGCCGG TCAGGTAACG AATGAATAA This corresponds to the amino acid sequence <SEQ ID 2556; ORF 746. a>: a746.pep 1 MΞEΝKQΝEVL SGYEQLKRRΝ RRRLVTAΞCL VAASCILLAA ALSSGPAEQT 51 AGETΞGVEΝK AAGAAQTPAL KSAADKPQDL AGEDKPΞAAD SEISEPEΝVG 101 APLVLIΝDRL EDSΝIKGLEA SEKLQQAETA KTAPKQAKQR AAEKVPATAD 151 ΞTDTVAVEKP KRTAETKPQK AERTAKAKPK AKETKTAEKV ADKPKTAAEK 201 TKPDTAKSDΞ AVKEAKKADK AESKKTAEKD RSDGKKHETA QKTDKADKTK 251 TAEKEKSGKK AAIQAGYAEK ERALSLQRKM KAAGIDSTIT EIMTDΝGKVY 301 RVKSSΝYKΝA RDAERDLΝKL RVHGIAGQVT ΝE* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 746 shows 99.7% identity over a 332 aa overlap with a predicted ORF (ORF 746) from N meningitidis: a746/m746; 99.7% identity in 332 aa overlap 10 20 30 40 50 60 a746.pep MSEΝKQΝEVLSGYEQLKRRΝRRRLVTASCLVAAΞCILLAAALSSGPAEQTAGETSGVEΝK 11111111111111 IM 11111111111111111 ii 111111111111111111111111 m746 SEΝKQΝEVLSGYEQLKRRΝRRRLVTASCLVAASCILLAAALSSGPAEQTAGETSGVEΝK 10 20 30 40 50 60 70 80 90 100 110 120 a746.pep AAGAAQTPALKΞAADKPQDLAGEDKPSAADSEISEPENVGAPLVLINDRLEDSNIKGLEA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I m746 AAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAPLVLINERLEDSNIKGLEA 70 80 90 100 110 120 130 140 150 160 170 • 180 a746. pep SEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKPKRTAETKPQKAERTAKAKPK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m746 SEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKPKRTAETKPQKAERTAKAKPK 130 140 150 160 170 180 190 200 210 220 230 240 a746. pep AKETKTAEKVADKPKTAAEKTKPDTAKΞDΞAVKEAKKADKAESKKTAEKDRSDGKKHETA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I m746 AKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKADKAESKKTAEKDRSDGKKHETA 190 200 210 220 230 240 250 260 270 280 290 300 a746.pep QKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSLQRKMKAAGIDSTITEIMTDNGKVY I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I m746 QKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSLQRKMKAAGIDSTITEIMTDNGKVY 250 260 270 280 290 300 310 320 330 a746. pep RVKΞSNYKNARDAERDLNKLRVHGIAGQVTNEX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m746 RVKSSNYKNARDAERDLNKLRVHGIAGQVTNEX 310 320 330 g747. seq not ound yet g747.pβp not found yet The following partial DNA sequence was identified in N meningitidis <SEQ ID 2557>: m747.seq 1 CTGACCCCTT GGGCGGATGC ATATGCAGAT TTGCGCGGCA AAACCAAAGT 51 GATGACGACC CAGATGGGTG CTTCCCGCGA TGTCAGCAAA AGCGCCAAAG 101 GTTGGAGTGT CGGTATCGGT CTGAATGTAG GCAAACAGTT GACCGACAGC 151 GTCGGTCTCG AGTTTGATCC ATACTACCGT CACAAAACAA TCTACAAACC 201 CCGTGAGATT GTCTTGGACG GTGACAAAAC CAAAATGGGC CGCTCCAAAT 251 CCAACGAGTA CGGCTTCCGC GTAGCCGCAA CGTTCTATAG TCAATTAAAA 301 TCAAAATAG This corresponds to the amino acid sequence <SEQ ID 2558; ORF 747>: m747.pep 1 LTPWADAYAD LRGKTKVMTT QMGASRDVSK SAKGWSVGIG LΝVGKQLTDS 51 VGLEFDPYYR HKTIYKPREI VLDGDKTKMG RSKΞΝEYGFR VAATFYSQLK 101 SK* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2559>: a747.seq 1 CTAACCCCTT GGGCGGATGC ATATGCAGAT TTGCGCGGCA AAACCAAAGT 51 GATGACGACC CAGATGTGTG CTTCCCGCGA TGTCAGCAAA AGCGCCAAAG 101 GTTGGAGTGT CGGTATCGGT CTGAATGTAG GCAAACAGTT GACCGACAGC 151 GTCGGTCTCG AGTTTGATCC ATACTACCGT CACAAAACAA TCTGCAAACC 201 CCGTGAGATT GTTTTGGACG GCGACAAAAC CAAAATGGGC CGCTCCAAAT 251 CCAACGAGTA CGGCTTCCGC GTAACCGCAA CGTTCTATAG TCAATTAAAA 301 TCAAAGTAG This corresponds to the amino acid sequence <SEQ ID 2560; ORF 747.a>: a747.pep 1 LTPWADAYAD LRGKTKVMTT QMCAΞRDVSK SAKGWSVGIG LΝVGKQLTDS 51 VGLEFDPYYR HKTICKPREI VLDGDKTKMG RSKSΝEYGFR VTATFYSQLK 101 SK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N. meningitidis ORF 747 shows 97.1% identity over a 102 aa overlap with a predicted ORF (ORF 746) from N meningitidis: a747/m747 97.1% identity n 102 aa overlap 10 20 30 40 50 60 a747.pep LTPWADAYADLRGKTKVMTTQMCASRDVΞKSAKGWSVGIGLΝVGKQLTDSVGLEFDPYYR I I I I I II I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I ιrι747 LTPWADAYADLRGKTKVMTTQMGASRDVSKΞAKGWSVGIGLΝVGKQLTDSVGLEFDPYYR 10 20 30 40 50 60 70 80 90 100 a747.pep HKTICKPREIVLDGDKTKMGRSKSΝEYGFRVTATFYSQLKΞKX llll I I I I II I II I I I I I II I I I I I I I I I Ml I I I I I I I I I I m 747 HKTIYKPREIVLDGDKTKMGRSKSΝEYGFRVAATFYSQLKSKX 70 80 90 100 a747/m80195 gi 1150271 (M80195) outer membrane protein [Neisseria meningitidis] Length = 272 Score = 59.3 bits (141), Expect = θe-09 Identities = 29/99 (29%), Positives = 51/99 (51%), Gaps = 4/99 (4%) Query: 1 LTPWADAYADLRGKTKVMTTQMCAΞRDVSKSAKGWSVGIGLNVGKQLTDSVGLEFDPYYR 60 + PW++ DL + K+ T +D+++ GW G+G N+GK+L +S +E P+Y+ Sbjct: 174 INPWSEVKFDLNSRYKLNTGVTNLKKDINQKTNGWGFGLGANIGKKLGESASIEAGPFYK 233 Query: 61 HKTICKPREIVL DGD-KTKMGRSKSNEYGFRVTATF 95 +T + E + GD + ++ EYG RV F Sb j ct: 234 QRTYKESGEFSVTTKSGDVΞLTIPKTΞIREYGLRVGIKF 272 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2561>: g748.seq 1 ATGAGTCAAA ACCAACCCGC ACAACCGACC AAACGCAATC TGTTCAAAAC 51 CGCCCTTGCC GTCGGCGCAA TCGGCGCAAT CGGAGGTTAT TTCGGCGGCA 101 AAAAACAGGG CGAAACCGCC GAACGCACCG CCGAAAGCCA ACACTCGCCC 151 CAAGCCTATC CCTGCTACGG CGAACATCAG GCAGGTATCG TTACGCCGCG 201 GCAGGCGTTT TCCATTATGT GCGCCTTCGA CGTAACCGCG CAAAGTGCCA 251 AGCAGCTGGA AAACCTGTTC CGCACACTGA CCGCCCGCAT CGAGTTTCTC 301 ACCCAAGGCG GAGAATACCA AGACGGCGAC GACAAACTCC CGTCAGCCGG 351 CAGCGGCATT TTGGGTAAAG CCTTCAACCC CGACGGATTG ACCGTTACCG 401 TGGGGGTGGG CAGCAGCCTG TTTGACGGCC GGTTCGGACT CAAAGACAAA 451 AAAACGGTTC ATTTGCAGGA AATGCGCGAC TTCCCCAACG ATAAGCTGCA 501 AAAAAGCTGG TGCGACGGCG ATTTGAGCCT GCAAATCTGC GCCTTCACCC 551 CCGAAACCTG CCAAACCGCC CTGCGCGACA TCATCAAACA CACCGCCCAA 601 ACCGCCGTCA TCCGCTGGAG TATCGACGGG TGGCAGCCTA AATCCGAACC 651 CGGCGCGATG GCGGCGCGCA ACCTGTTGGG CTTCCGAGAC GGCACGGGCA 701 ACCCCAAGGT TTCCGATCCC AAAACCGCCG ACGAGGTTTT ATGGACGGGC 751 GTGGCCGCCA ACAGCCTCGA CGAACCGGAG TGGGCGAAAA ACGGCAGCTA 801 TCAGGCAGTC CGCCTTATCC GCCGCTTTGT CGAGTTTTGG GACAGGACGC 851 CGCTTCAAGA GCAAACCGAC ATTTTCGGGC GGCGAAAATA CAGCGGGGCG 901 CCGATGGACG GCAAAAAAGA AGCCGACCAA CCGGATTTCG CCAAAGACCC 951 CGAGGGTGAT ATCACGCCCA AAGACAGCCA TATGCGCCTG GCGAATCCGC 1001 GCGATCCCGA ATTCCTCAAA AAACACTGCC TCTTCCGCCG CGCCTACAGC 1051 TATTCTCGCG GACCCGCCTC AAGCGGACAG CTTGATGTCG GGCTGGTGTT 1101 CGTCTGCTAT CAGGCAAATC TTGCCGACGG TTTCATCTTC GTGCAAAACC 1151 TCCTCAACGG CGAACCGCTG GAAGAATACA TCAGCCCCTT CGGCGGCGGC 1201 TATTTCTTCG TCTTGCCCGG CGTGGGAAAA GGCGGATTCT TGGGACAAGG 1251 GCTGCCGGGC GTATAA This corresponds to the amino acid sequence <SEQ ID 2562; ORF 748.ng>: g748.pep 1 MΞQΝQPAQPT KRΝLFKTALA VGAIGAIGGY FGGKKQGETA ERTAESQHSP 51 QAYPCYGEHQ AGIVTPRQAF ΞIMCAFDVTA QΞAKQLEΝLF RTLTARIEFL 101 TQGGEYQDGD DKLPSAGSGI LGKAFΝPDGL TVTVGVGΞSL FDGRFGLKDK 151 KTVHLQEMRD FPΝDKLQKSW CDGDLSLQIC AFTPETCQTA LRDIIKHTAQ 201 TAVIRWSIDG WQPKSEPGAM AARΝLLGFRD GTGΝPKVSDP KTADEVLWTG 251 VAAΝΞLDEPE WAKΝGSYQAV RLIRRFVEFW DRTPLQEQTD IFGRRKYSGA 301 PMDGKKEADQ PDFAKDPEGD ITPKDSHMRL AΝPRDPEFLK KHCLFRRAYS 351 YSRGPASSGQ LDVGLVFVCY QAΝLADGFIF VQΝLLΝGEPL EEYIΞPFGGG 401 YFFVLPGVGK GGFLGQGLPG V* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2563>: m748.seq 1 ATGAGCAAAA AACAACCCGC ACAACCGACC AGGCGCACTC TTTTTAAAAC 51 CGCGATCGCA GCCGGAGCAG TCGGCGCAAT CGGAGGTTAT CTCGGCGGCA 101 AAAAACAGGG CGAAACCGCC GAACGCACCG CCGAAAGCCA ACACTCGCCC 151 CAAGCCTATC CCTGCTACGG CGAACATCAG GCAGGCATCG TTACGCCGCA 201 GCAGGCGTTT TCGATTATGT GCGCCTTCGA CGTAACCGCG CAAAGTGCCA 251 AGCAGCTGGA AAACCTGTTC CGCACGCTGA CCGCCCGCAT CGAGTTTCTC 301 ACCCAAGGCG GCGAATACCA AGACGGCGAC GACAAACTTC CGCCAGCCGG 351 CAGCGGCATT TTGGGCAAAG CCTTCAACCC CGACGGGTTG ACCGTTACCG 401 TGGGGGTGGG CAGCAGCCTG TTTGACGGCC GGTTCGGACT CAAAGACAAA 451 AAACCGATTC ATTTGCAGGA AATGCGCGAC TTCTCCAACG ATAAGCTGCA 501 AAAAAGCTGG TGCGACGGCG ATTTGAGCCT GCAAATCTGT GCCTTCACCC 551 CCGAAACCTG CCAAGCCGCC CTGCGCGACA TCATCAAACA CACCGTCCAA 601 ACCGCCGTTA TCCGTTGGAG TATCGACGGG TGGCAGCCCA AATCCGAACC 651 CGGCGCGATG GCGGCGCGCA ACCTGTTGGG CTTCAGGGAC GGCACGGGCA 701 ACCCCAAAGT TTCCGATCCC AAAACTGCCG ACGAGGTTTT GTGGACGGGG 751 GTGGCCGCCA ACAGCCTCGA CGAACCGGAG TGGGCGAAAA ACGGCAGCTA 801 TCAGGCAGTC CGCCTTATCC GCCACTTTGT CGAGTTTTGG GACAGGACGC 851 CGCTTCAAGA GCAAACCGAC ATTTTCGGGC GGCGCAAATA CAGCGGTGCG 901 CCGATGGACG GCAAAAAAGA AGCCGACCAA CCGGATTTTG CCAAAGACCC 951 CGAGGGTGAT ATCACGCCCA AAGACAGCCA TATACGCCTG GCGAATCCGC 1001 GCGATCCCGA ATTCCTCAAA AAACACCGCC TCTTCCGCCG CGCCTACAGC 1051 TATTCGCGCG GACTCGCCTC AAGCGGACAG CTTGATGTCG GGCTGGTGTT 1101 CGTCTGCTAT CAGGCAAACC TTGCCGACGG ATTCATCTTC GTGCAAAACC 1151 TCCTCAACGG CGAACCGCTG GAAGAATACA TCAGCCCCTT CGGCGGCGGC 1201 TATTTCTTCG TCTTGCCCGG CGTGGAAAAA GGCGGCTTTT TGGGGCAAGG 1251 GCTGCTGGGC GTATAA This corresponds to the amino acid sequence <SEQ ID 2564; ORF 748>: m748.pep 1 MSKKQPAQPT RRTLFKTAIA AGAVGAIGGY LGGKKQGETA ERTAESQHSP 51 QAYPCYGEHQ AGIVTPQQAF SIMCAFDVTA QSAKQLEΝLF RTLTARIEFL 101 TQGGEYQDGD DKLPPAGSGI LGKAFΝPDGL TVTVGVGSSL FDGRFGLKDK 151 KPIHLQEMRD FSΝDKLQKSW CDGDLΞLQIC AFTPETCQAA LRDIIKHTVQ 201 TAVIRWSIDG WQPKSEPGAM AARΝLLGFRD GTGΝPKVSDP KTADEVLWTG 251 VAAΝSLDEPE WAKΝGSYQAV RLIRHFVEFW DRTPLQEQTD IFGRRKYSGA 301 PMDGKKEADQ PDFAKDPEGD ITPKDSHIRL AΝPRDPEFLK KHRLFRRAYS 351 YSRGLASSGQ LDVGLVFVCY QAΝLADGFIF VQΝLLΝGEPL EEYISPFGGG 401 YFFVLPGVEK GGFLGQGLLG V* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 748 shows 95.0% identity over a 421 aa overlap with a predicted ORF (ORF 748) from N gonorrhoeae m748/g748 95.0% identity in 421 aa overlap 10 20 30 40 50 60 m748.pep MSKKQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKQGETAERTAESQHΞPQAYPCYGEHQ I M M I I I I I M M I I I I M M M l I I I I M l I I I I I I I I I I I I I I I I I I I I I I I I I I I I g748 MSQΝQPAQPTKRΝLFKTALAVGAIGAIGGYFGGKKQGETAERTAESQHSPQAYPCYGEHQ 10 20 30 40 50 60 70 80 90 100 110 120 m748.pep AGIVTPQQAFSIMCAFDVTAQSAKQLEΝLFRTLTARIEFLTQGGEYQDGDDKLPPAGSGI I I I I I I : I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g748 AGIVTPRQAFΞIMCAFDVTAQΞAKQLEΝLFRTLTARIEFLTQGGEYQDGDDKLPSAGSGI 70 80 90 100 110 120 130 140 150 160 170 180 m748, ■pep LGKAFΝPDGLTVTVGVGΞΞLFDGRFGLKDKKPIHLQEMRDFΞΝDKLQKSWCDGDLSLQIC I II I I I I I I I I I I I I I I I I I I I I I II I I I I I : I I I I II I I I I I I I I I I I I I I I I I I I I g748 LGKAFΝPDGLTVTVGVGSSLFDGRFGLKDKKTVHLQEMRDFPΝDKLQKSWCDGDLSLQIC 130 140 150 160 170 180 190 200 210 220 230 240 m748. •Pep AFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPGAMAARΝLLGFRDGTGΝPKVSDP II I I I I I M I I I I I I I I I : I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g748 AFTPETCQTALRDIIKHTAQTAVIRWSIDGWQPKΞEPGAMAARNLLGFRDGTGNPKVΞDP 190 200 210 220 230 240 250 260 270 280 290 300 Itl7 8.pep KTADEVLWTGVAANSLDEPEWAKNGΞYQAVRLIRHFVEFWDRTPLQEQTDIFGRRKYΞGA I I I I I I I I I I I I II I I I I I II I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I g 48 KTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRRFVEFWDRTPLQEQTDIFGRRKYSGA 250 260 270 280 290 300 310 320 330 340 350 360 m748.pep PMDGKKEADQPDFAKDPEGDITPKDSHIRLANPRDPEFLKKHRLFRRAYSYSRGLASΞGQ I I I I I I I I I I I I II I I I I I II I I I I I I : I I I I I I I I I I I I II I I I I I I I I I I I I I I I I g 48 PMDGKKEADQPDFAKDPEGDITPKDSHMRLANPRDPEFLKKHCLFRRAYSYSRGPASSGQ 310 320 330 340 350 360 370 380 390 400 410 420 m748.pep LDVGLVFVCYQANLADGFIFVQNLLNGEPLEEYISPFGGGYFFVLPGVEKGGFLGQGLLG I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g 48 LDVGLVFVCYQANLADGFIFVQNLLNGEPLEEYISPFGGGYFFVLPGVGKGGFLGQGLPG 370 380 390 400 410 420 m748.pep VX I I g748 VX The following partial DNA sequence was identified in N meningitidis <SEQ ID 2565>: a748.seq 1 ATGAGCAAAA ACCAACCCGC ACAACCGACC AGGCGCACTC TTTTTAAAAC 51 CGCGATCGCA GCTGGAGCAG TCGGCGCAAT CGGAGGTTAT CTCGGCGGCA 101 AAAAACGGGG CGAAACCGCC GAACGCACCG CCGAAAGCCA ACACTCGCCC 151 CAAGCCTATC CCTGCTACGG CGAACATCAG GCAGGCATCG TTACGCCGCA 201 GCAGGCGTTT TCGATTATGT GCGCCTTCGA CGTAACCGCG CAAAGTGCCA 251 AGCAGCTGGA AAACCTGTTC CGCACGCTGA CCGCCCGCAT CGAGTTTCTC 301 ACCCAAGGCG GCGAATACCA AGACGGCGAC GACAAACTTC CGCCAGCCGG 351 CAGCGGCATT TTGGGCAAAG CCTTCAACCC CGACGGGTTG ACCGTTACCG 401 TGGGGGTGGG CAGCAGCCTG TTTGACGGCC GGTTCGGACT CAAAGACAAA 451 AAACCGATTC ATTTGCAGGA AATGCGCGAC TTCTCCAACG ATAAGCTGCA 501 AAAAAGCTGG TGCGACGGCG ATTTGAGCCT GCAAATCTGT GCCTTCACCC 551 CCGAAACCTG CCAAGCCGCC CTGCGCGACA TCATCAAACA CACCGTCCAA 601 ACCGCCGTTA TCCGCTGGAG TATCGACGGG TGGCAGCCTA AATCCGAACC 651 CGGCGCGATG GCGGCGCGCA ACCTGTTGGG CTTCCGCGAC GGCACGGGCA 701 ACCCCAAAGT TTCCGACCCC AAAACTGCCG ACGAGGTTTT GTGGACGGGG 751 GTGGCCGCCA ACAGCCTCGA CGAACCGGAG TGGGCGAAAA ACGGCAGCTA 801 TCAGGCAGTC CGCCTTATCC GCCACTTTGT TGAGTTTTGG GACAGGACGC 851 CGCTTCAAGA GCAAACCGAC ATTTTCGGGC GGCGCAAATA CAGCGGCGCG 901 CCGATGGACG GCAAAAAAGA AGCCGACCAA CCGGATTTTG CCAAAGACCC 951 CGAGGGGAAT ACCACGCCCA AAGACAGCCA TATACGCCTG GCGAATCCGC 1001 GCGATCCCGA GTTCCTTAAA AAACACCGCC TCTTCCGCCG CGCCTACAGC 1051 TATTCGCGCG GACTCGCCTC AAGCGGACAG CTTGATGTCG GGCTGGTGTT 1101 CGTCTGCTAT CAGGCAAACC TTGCCGACGG ATTCATCTTC GTGCAAAACC 1151 TCCTCAACGG CGAACCGCTG GAAGAATACA TCAGCCCCTT CGGCGGCGGC 1201 TATTTCTTCG TCTTGCCCGG CGTGGAAAAA GGCGGCTTTT TGGGGCAAGG 1251 GCTGCTGGGC GTATAA This corresponds to the amino acid sequence <SEQ ID 2566; ORF 748. a>: a748.pep 1 MSKΝQPAQPT RRTLFKTAIA AGAVGAIGGY LGGKKRGETA ERTAESQHSP 51 QAYPCYGEHQ AGIVTPQQAF SIMCAFDVTA QΞAKQLEΝLF RTLTARIEFL 101 TQGGEYQDGD DKLPPAGSGI LGKAFΝPDGL TVTVGVGSSL FDGRFGLKDK 151 KPIHLQEMRD FSΝDKLQKSW CDGDLSLQIC AFTPETCQAA LRDIIKHTVQ 201 TAVIRWSIDG WQPKSEPGAM AARΝLLGFRD GTGΝPKVΞDP KTADEVLWTG 251 VAAΝSLDEPE WAKΝGSYQAV RLIRHFVEFW DRTPLQEQTD IFGRRKYSGA 301 PMDGKKEADQ PDFAKDPEGΝ TTPKDSHIRL AΝPRDPEFLK KHRLFRRAYS 351 YSRGLASSGQ LDVGLVFVCY QAΝLADGFIF VQΝLLΝGEPL EEYISPFGGG 401 YFFVLPGVEK GGFLGQGLLG V* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 748 shows 99.0% identity over a 421 aa overlap with a predicted ORF (ORF 748) from N meningitidis: a748/m748 99.0% identity in 421 aa overlap 10 20 30 40 50 60 a748.pep MSKΝQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKRGETAERTAESQHΞPQAYPCYGEHQ I I IM I I I I I I I I I I I I I I I I II I I I I I I I I I I I I : I I I I I I I II I I I I I I I I I I I I I I I m748 MSKKQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKQGETAERTAESQHΞPQAYPCYGEHQ 10 20 30 40 50 60 70 80 90 100 110 120 a748.pep AGIVTPQQAFSIMCAFDVTAQSAKQLEΝLFRTLTARIEFLTQGGEYQDGDDKLPPAGSGI II I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m7 8 AGIVTPQQAFSIMCAFDVTAQSAKQLEΝLFRTLTARIEFLTQGGEYQDGDDKLPPAGΞGI 70 80 90 100 110 120 130 140 150 160 170 180 a748. pep LGKAFΝPDGLTVTVGVGΞΞLFDGRFGLKDKKPIHLQEMRDFSΝDKLQKSWCDGDLSLQIC I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m748 LGKAFΝPDGLTVTVGVGSSLFDGRFGLKDKKPIHLQEMRDFSΝDKLQKΞWCDGDLSLQIC 130 140 150 160 170 180 190 200 210 220 230 240 a748.pep AFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPGAMAARΝLLGFRDGTGΝPKVSDP I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I m748 AFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKΞEPGAMAARΝLLGFRDGTGΝPKVSDP 190 200 210 220 230 240 250 260 270 280 290 300 a748.pep KTADEVLWTGVAAΝSLDEPEWAKΝGSYQAVRLIRHFVEFWDRTPLQEQTDIFGRRKYΞGA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I m748 KTADEVLWTGVAAΝSLDEPEWAKΝGSYQAVRLIRHFVEFWDRTPLQEQTDIFGRRKYSGA 250 260 270 280 290 300 310 320 330 340 350 360 a748.pep PMDGKKEADQPDFAKDPEGΝTTPKDSHIRLAΝPRDPEFLKKHRLFRRAYΞYSRGLASSGQ I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m748 PMDGKKEADQPDFAKDPEGDITPKDΞHIRLAΝPRDPEFLKKHRLFRRAYSYSRGLASSGQ 310 320 330 340 350 360 370 380 390 400 410 420 a748.pep LDVGLVFVCYQAΝLADGFIFVQΝLLΝGEPLEEYISPFGGGYFFVLPGVEKGGFLGQGLLG I II I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I rrι748 LDVGLVFVCYQAΝLADGFIFVQΝLLΝGEPLEEYISPFGGGYFFVLPGVEKGGFLGQGLLG 370 380 390 400 410 420 a748.pep VX I I m748 VX The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 2567>: g749.seq 1 ATGAGAAAAT TCAATTTGAC CGCATTGTCC GTGATGCTTG CCTTGGGTTT 51 GACCGCGTGC CAGCCGCCGG AGGCGGAGAA AGCCGCGCCG GCCGCGTCCG 101 GTGAGACCCA ATCCGCCAAC GAAGGCGGTT CGGTCGGTAT CGCCGTCAAC 151 GACAATGCCT GCGAACCGAT GAATCTGACC GTGCCGAGCG GACAGGTTGT 201 GTTCAATATT AAAAACAACA GCGGCCGCAA GCTCGAATGG GAAATCCTGA 251 AGGGCGTGAT GGTGGTGGAC GAACGCGAAA ATATCGCCCC GGGGCTTTCC 301 GACAAAATGA CCGTAAccct GCTGCCGGGC GAATACGAAA TGACCTGCGG 351 CCTTTTGACC AATCCGCGCG GCAAGCTGGT GGTAGCCGAC AGCGGCTTTA 401 AAGACACCGC CAACGAAGCG GATTTGGAAA AACTGCCCCA ACCGCTCGCC 451 GACTATAAAG CCTACGTTCA AGGCGAGGTT AAAGAGCTGG CGGCGAAAAC 501 CAAAACCTTT ACCGAAGCCG TCAAAGCAGG CGACATTGAA AAGGCGAAAT 551 CCCTGTTTGC CGCCACCCGC GTCCATTACG AACGCATCGA ACCGATTGCC 601 GAGCTTTTCA GCGAACTCGA CCCCGTCATC GATGCGTGTG AAGACGACTT 651 CAAAGACGGT GCGAAAGATG CCGGGTTTAC CGGCTTCCAC CGTATCGAAC 701 ACGCCCTTTG GGTGGAAAAA GACGTATCCG GCGTGAAGGA AACCGCGGCC 751 AAACTGATGA CCGATGTCGA AGCCCTGCAA AAAGAAATCG ACGCATTGGC 801 GttCCCtCCG GGCAAAGTGG TCGGCGGCGC GTCCGAACTG ATTGAAGAAG 851 CGGCGGGCAG TAAAATCAGC GGCGAAGAAG ACCgttaCAG CCACACCGAT 901 TTGAGCGACT TCCAAGCTAA TGCGGACGGA TCTAAAAAAA TCGTCGATTT 951 GTTCCGTCCG TTGATTGAGG CCAAAAACAA AGCCTTGTTG GAAAAAACCG 1001 ATACCAACTT CAAACAGGTC AACGAAATTC TGGCGAAATA CCGCACCAAA 1051 GACGGTTTTG AAACCTACGA CAAGCTGAGC GAAGCCGACC GCAAAGCATT 1101 ACAGGCTCCT ATTAACGCGC TTGCCGAAGA CCTTGCCCAA CTTCGCGGCA 1151 TACTCGGCTT GAAATAA This corresponds to the amino acid sequence <SEQ ID 2568; ORF 749.ng>: g749.pep 1 MRKFNLTALS VMLALGLTAC QPPEAEKAAP AAΞGETQSAN EGGSVGIAVN 51 DNACEPMNLT VPSGQWFNI KNNSGRKLEW EILKGVMWD ERENIAPGLS 101 DKMTVTLLPG EYEMTCGLLT NPRGKLVVAD SGFKDTANEA DLEKLPQPLA 151 DYKAYVQGEV KELAAKTKTF TEAVKAGDIE KAKSLFAATR VHYERIEPIA 201 ELFSELDPVI DACEDDFKDG AKDAGFTGFH RIEHALWVEK DVSGVKETAA 251 KLMTDVEALQ KEIDALAFPP GKWGGASEL IEEAAGSKIS GEEDRYSHTD 301 LΞDFQANADG SKKIVDLFRP LIEAKNKALL EKTDTNFKQV NEILAKYRTK 351 DGFETYDKLS EADRKALQAP INALAEDLAQ LRGILGLK* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2569>: 1 ATGAGAAAAT TCAATTTGAC CGCATTGTCC GTGATGCTTG CCTTAGGTTT 51 GACCGCGTGC CAGCCGCCGG AGGCGGAGAA AGCTGCGCCG GCAGCGTCCG 101 GTGAGGCGCA AACCGCCAAC GAGGGCGGTT CGGTCAGTAT CGCCGTCAAC 151 GACAATGCCT GCGAACCGAT GGAACTGACC GTGCCGAGCG GACAGGTTGT 201 GTTCAATATT AAAAACAACA GCGGCCGCAA GCTCGAATGG GAAATCCTGA 251 AAGGCGTGAT GGTGGTGGAC GAGCGCGAAA ACATCGCCCC CGGACTTTCC 301 GATAAAATGA CCGTCACCCT GTTGCCGGGC GAATACGAAA TGACTTGCGG 351 TCTTTTGACC AATCCGCGCG GCAAGCTGGT GGTAACCGAC AGCGGCTTTA 401 AAGACACCGC CAACGAAGCG GATTTGGAAA AACTGTCCCA ACCGCTCGCC 451 GACTATAAAG CCTACGTTCA AGGCGAGGTT AAAGAGCTGG TGGCGAAAAC 501 CAAAACTTTT ACCGAAGCCG TCAAAGCAGG CGACATTGAA AAGGCGAAAT 551 CCCTGTTTGC CGACACCCGC GTCCATTACG AACGCATCGA ACCGATTGCC 601 GAGCTTTTCA GCGAACTCGA CCCCGTCATC GATGCGCGTG AAGACGACTT 651 CAAAGACGGC GCGAAAGATG CCGGATTTAC CGGCTTTCAC CGTATCGAAT 701 ACGCCCTTTG GGTGGAAAAA GACGTGTCCG GCGTGAAGGA AATTGCAGCG 751 AAACTGATGA CCGATGTCGA AGCCCTGCAA AAAGAAATCG ACGCATTGGC 801 GTTTCCTCCG GGCAAGGTGG TCGGCGGCGC GTCCGAACTG ATTGAAGAAG 851 TGGCGGGCAG TAAAATCAGC GGCGAAGAAG ACCGGTACAG CCACACCGAT 901 TTGAGCGACT TCCAAGCCAA TGTGGACGGA TCTAAAAAAA TCGTCGATTT 951 GTTCCGTCCG CTGATCGAGG CCAAAAACAA AGCCTTGTTG GAAAAAACCG 1001 ATACCAACTT CAAACAGGTC AACGAAATTC TGGCGAAATA CCGGACTAAA 1051 GACGGTTTTG AAACCTACGA CAAGCTGGGC GAAGCCGACC GCAAAGCGTT 1101 ACAGGCCTCT ATTAACGCGC TTGCCGAAGA CCTTGCCCAA CTTCGCGGCA 1151 TACTCGGCTT GAAATAA This corresponds to the amino acid sequence <SEQ ID 2570; ORF 749>: m749.pep 1 MRKFNLTALS VMLALGLTAC QPPEAEKAAP AAΞGEAQTAN EGGSVSIAVN 51 DNACEPMELT VPSGQWFNI KNNSGRKLEW EILKGVMWD ERENIAPGLS 101 DKMTVTLLPG EYEMTCGLLT NPRGKLVVTD SGFKDTANEA DLEKLSQPLA 151 DYKAYVQGEV KELVAKTKTF TEAVKAGDIE KAKΞLFADTR VHYERIEPIA 201 ELFSELDPVI DAREDDFKDG AKDAGFTGFH RIEYALWVEK DVSGVKEIAA 251 KLMTDVEALQ KEIDALAFPP GKWGGASEL IEEVAGΞKIΞ GEEDRYSHTD 301 LSDFQANVDG SKKIVDLFRP LIEAKNKALL EKTDTNFKQV NEILAKYRTK 351 DGFETYDKLG EADRKALQAΞ INALAEDLAQ LRGILGLK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N. gonorrhoeae ORF 749 shows 96.1% identity over a 388 aa overiap with a predicted ORF (ORF 749) from N gonorrhoeae m749/g749 96.1% identity in 388 aa overlap 10 20 30 40 50 60 m749. pep MRKFΝLTALSVMLALGLTACQPPEAEKAAPAASGEAQTAΝEGGSVSIAVΝDΝACEPMELT II I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I MM I I I I I I I : I I I I I I I I II I : I I g749 MRKFΝLTALSVMLALGLTACQPPEAEKAAPAASGETQSAΝEGGSVGIAVΝDΝACEPMΝLT 10 20 30 40 50 60 70 80 90 100 110 120 m749.pep VPSGQWFNIKNNSGRKLEWEILKGVMWDERENIAPGLSDKMTVTLLPGEYEMTCGLLT I II I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I II II I I I I I I I I I I I I I g749 VPSGQWFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDKMTVTLLPGEYEMTCGLLT 70 80 90 100 110 120 130 140 150 160 170 180 m749 . pep NPRGKLVVTDΞGFKDTANEADLEKLSQPLADYKAYVQGEVKELVAKTKTFTEAVKAGDIE I I I I I I I I M I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I g749 NPRGKLWADSGFKDTANEADLEKLPQPLADYKAYVQGEVKELAAKTKTFTEAVKAGDIE 130 140 150 160 170 180 190 200 210 220 230 240 m749 . pep KAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFKDGAKDAGFTGFHRIEYALWVEK l l l l l l l I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M l I I I I I g749 KAKSLFAATRVHYERIEPIAELFSELDPVIDACEDDFKDGAKDAGFTGFHRIEHALWVEK 190 200 210 220 230 240 250 260 270 280 290 300 πl749 . pep DVSGVKEIAAKLMTDVEALQKEIDALAFPPGKWGGASELIEEVAGΞKISGEEDRYSHTD l l l l l l l I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I g749 DVΞGVKETAAKLMTDVEALQKEIDALAFPPGKWGGASELIEEAAGSKI SGEEDRYΞHTD 250 260 270 280 290 300 310 320 330 340 350 360 m749 . pep LSDFQANVDGSKKIVDLFRPLIEAKNKALLEKTDTNFKQVNEILAKYRTKDGFETYDKLG I I I I I I M l I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : g749 LSDFQANADGSKKIVDLFRPLIEAKNKALLEKTDTNFKQVNEILAKYRTKDGFETYDKLS 310 320 330 340 350 360 370 380 389 m749.pep EADRKALQASINALAEDLAQLRGILGLKX II III I I I I I I I I I I I I I I g749 EADRKALQAPINALAEDLAQLRGILGLKX 370 380 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2571>: a749.seq 1 ATGAGAAAAT TCAATTTGAC CGCATTGTCC GTGATGCTTG CCTTAGGTTT 51 GACCGCGTGC CAGCCGCCGG AGGCGGAGAA AGCTGCGCCG GCAGCGTCCG 101 GTGAGGCGCA AACCGCCAAC GAGGGCGGTT CGGTCAGTAT CGCCGTCAAC 151 GACAATGCCT GCGAACCGAT GGAACTGACC GTGCCGAGCG GACAGGTTGT 201 GTTCAATATT AAAAACAACA GCGGCCGCAA GCTCGAATGG GAAATCCTGA 251 AAGGCGTGAT GGTGGTGGAC GAGCGCGAAA ACATCGCCCC CGGACTTTCC 301 GATAAAATGA CCGTCACCCT GTTGCCGGGC GAATACGAAA TGACTTGCGG 351 TCTTTTGACC AATCCGCGCG GCAAGCTGGT GGTAACCGAC AGCGGCTTTA 401 AAGACACCGC CAACGAAGCG GATTTGGAAA AACTGTCCCA ACCGCTCGCC 451 GACTATAAAG CCTATGTTCA AGGCGAAGTC AAAGAGCTGG TGGCGAAAAC 501 CAAAACCTTT ACCGAAGCCG TCAAAGCAGG CGACATTGAA AAGGCGAAAT 551 CCCTGTTTGC CGACACCCGC GTCCATTACG AACGCATCGA ACCGATTGCC 601 GAGCTTTTCA GCGAACTCGA CCCCGTCATC GATGCGCGTG AAGACGACTT 651 CAAAGACGGC GCGAAAGATG CCGGATTTAC CGGCTTCCAC CGTATCGAAT 701 ACGCCCTTTG GGTGGAAAAA GACGTGTCCG GCGTGAAGGA AATTGCAGCG 751 AAACTGATGA CCGATGTCGA AGCCCTGCAA AAAGAAATCG ACGCATTGGC 801 GTTTCCTCCG GGCAAGGTGG TCGGCGGCGC GTCCGAACTG ATTGAAGAAG 851 TGGCGGGCAG TAAAATCAGC GGCGAAGAAG ACCGGTACAG CCACACCGAT 901 TTGAGCGACT TCCAAGCCAA TGTGGACGGA TCGAAAAAAA TCGTCGATTT 951 GTTCCGTCCG TTGATCGAGA CCAAAAACAA AGCCTTGTTG GAAAAAACCG 1001 ATACCAACTT CAAACAGGTC AACGAAATTC TGGCGAAATA CCGGACTAAA 1051 GACGGTTTTG AAACCTACGA CAAGCTGGGC GAAGCCGACC GCAAAGCGTT 1101 ACAGGCCTCT ATTAACGCGC TTGCCGAAGA CCTTGCCCAA CTTCGCGGCA 1151 TACTCGGCTT GAAATAA This corresponds to the amino acid sequence <SEQ ID 2572; ORF 749. a>: a749.pep 1 MRKFNLTALS VMLALGLTAC QPPEAEKAAP AASGEAQTAN EGGSVSIAVN 51 DNACEPMELT VPSGQWFNI KNNSGRKLEW EILKGVMWD ERENIAPGLS 101 DKMTVTLLPG EYEMTCGLLT NPRGKLVVTD SGFKDTANEA DLEKLSQPLA 151 DYKAYVQGEV KELVAKTKTF TEAVKAGDIE KAKΞLFADTR VHYERIEPIA 201 ELFSELDPVI DAREDDFKDG AKDAGFTGFH RIEYALWVEK DVSGVKEIAA 251 KLMTDVEALQ KEIDALAFPP GKWGGASEL IEEVAGSKIS GEEDRYSHTD 301 LSDFQANVDG SKKIVDLFRP LIETKNKALL EKTDTNFKQV NEILAKYRTK 351 DGFETYDKLG EADRKALQAΞ INALAEDLAQ LRGILGLK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 749 shows 99.7% identity over a 388 aa overlap with a predicted ORF (ORF 749) from N meningitidis: a749/m749 99.7% identity in 388 aa overlap 10 20 30 40 50 60 a749.pep MRKFΝLTALSVMLALGLTACQPPEAEKAAPAASGEAQTAΝEGGSVSIAVΝDΝACEPMELT I I I I I I I I I II I II I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m749 MRKFΝLTALSVMLALGLTACQPPEAEKAAPAASGEAQTAΝEGGSVΞIAVΝDΝACEPMELT 10 20 30 40 50 60 70 80 90 100 110 120 a749.pep VPSGQWFΝIKΝΝSGRKLEWEILKGVMWDEREΝIAPGLSDKMTVTLLPGEYEMTCGLLT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I l l l l I I I I I I I I I I I I I I I I I I I I I I I I I I I 749 VPSGQVVFΝIKΝΝSGRKLEWEILKGVMVVDEREΝIAPGLSDKMTVTLLPGEYEMTCGLLT 70 80 90 100 110 120 130 140 150 160 170 180 a749 . pep ΝPRGKLWTDSGFKDTAΝEADLEKLSQPLADYKAYVQGEVKELVAKTKTFTEAVKAGDIE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m749 ΝPRGKLWTDSGFKDTAΝEADLEKLSQPLADYKAYVQGEVKELVAKTKTFTEAVKAGDIE 130 140 150 160 170 180 190 200 210 220 230 240 a749.pep KAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFKDGAKDAGFTGFHRIEYALWVEK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m749 KAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFKDGAKDAGFTGFHRIEYALWVEK 190 200 210 220 230 240 250 260 270 280 290 300 a749.pep DVSGVKEIAAKLMTDVEALQKEIDALAFPPGKWGGASELIEEVAGΞKISGEEDRYSHTD I II I I I I I I I I I I II I I I I I I I I I II I III I I I I II I II I I I I III I I I I I I I I I I II I I m749 DVΞGVKEIAAKLMTDVEALQKEIDALAFPPGKWGGASELIEEVAGSKISGEEDRYSHTD 250 260 270 280 290 300 310 320 330 340 350 360 a749.pep LSDFQAΝVDGSKKIVDLFRPLIETKΝKALLEKTDTΝFKQVΝEILAKYRTKDGFETYDKLG I I I I I I I I I I I I I I I I I I I I I I M I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m749 LSDFQAΝVDGSKKIVDLFRPLIEAKΝKALLEKTDTΝFKQVΝEILAKYRTKDGFETYDKLG 310 320 330 340 350 360 370 380 389 a749.pep EADRKALQASINALAEDLAQLRGILGLKX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m749 EADRKALQASINALAEDLAQLRGILGLKX 370 380 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2573>: g750.seq 1 GTGAAACCGC GTTTTTATTG GGCAGcctGC GCCGTCCTGC CGGCCGCCTG 51 TTCGCCCGAA CCTGCCGCCG AAAAAACTGT ATccgCCGCA TCCCAAGCCG 101 CATCCACACC TGTCGCCACG CTGACCGTGC CGACCGCGCG GGGCGATGCC 151 GTTGTGCCGA AGAATCCCGA ACgcgtcgcc gtgtAcgaCt ggGCGGCGTt 201 ggaTACGCTG ACCGAGCCGG GCGTGAATGT GGGCGCAACC ACCGCGCCGG 251 TGCGCGTGGA CTATTTGCAG CCTGCATTTG ACAAGGCGGC AACGGTGGGG 301 ACGCTGTTTG AGCCCGATTG CGAATCCCTG CACCGCCACA ATCCGCAGTT 351 TGTCATTACC GGCGGGCCGG GTGCGGAAGC GTATGAACAG TTGGCGAAAA 401 ACGCGACCAC CATAGATTTG ACGGTGGACA ACGGCAATAT CCGCACCAGC 451 GGCGAGAAGC AGATGGAGAC CCTGTCGCGG ATTTTCGGTA AGGAAGCGCG 501 CGTGGCGGAA TTGAATGCGC AGATTGACGC GCTGTTCGCC CAAAAGCGCG 551 AAGCCGCCAA AGGCAAAGGA CGCGGGCTGG TGCTGTCGGT TACAGGCAAC 601 AAGGTGTCCG CCTTCGGCAC GCAATCGCGG TTGGCAAGTT GGATACACGG 651 CGACATCGGC CTGCCGCCCG TGGACGAATC TTTACGCAAC GAAGGGCACG 701 GGCAGCCCGT TTCCTTCGAA TACATCAAAG AGAAAAACCC CGGCTGGATT 751 TTCATCATCG ACCGCACCGC CGCCATCGGG CAGGAAGGGC CGGCTGCCGT 801 GGAAGTGTTG GATAACGCGC TGGTATGCGG CACGAACGCT TGGAAGCGCA 851 AGCAAATCAT CGTCATGCCT GCCGCGAACT ACATTGTCGC GGGCGGCGCG 901 CGGCAGTTGA TACAGGCGGC GGAACAGTTG AAGGCGGCGT TTGAAAAGGC 951 AGAACCCGTT GCGGCGCAGT AG This corresponds to the amino acid sequence <SEQ ID 2574; ORF 750.ng>: g750.pep 1 VKPRFYWAAC AVLPAACΞPE PAAEKTVSAA SQAASTPVAT LTVPTARGDA 51 VVPKNPERVA VYDWAALDTL TEPGVNVGAT TAPVRVDYLQ PAFDKAATVG 101 TLFEPDCESL HRHNPQFVIT GGPGAEAYEQ LAKNATTIDL TVDNGNIRTS 151 GEKQMETLΞR IFGKEARVAE LNAQIDALFA QKREAAKGKG RGLVLΞVTGN 201 KVSAFGTQΞR LASWIHGDIG LPPVDESLRN EGHGQPVΞFE YIKEKNPGWI 251 FIIDRTAAIG QEGPAAVEVL DNALVCGTNA WKRKQIIVMP AANYIVAGGA 301 RQLIQAAEQL KAAFEKAEPV AAQ* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2575>: m750.seq 1 GTGAAACCGC GTTTTTATTG GGCAGCCTGC GCCGTCCTGC TGACCGCCTG 51 TTCGCCCGAA CCTGCCGCCG AAAAAACTGT ATCCGCCGCA TCCGCATCTG 101 CCGCCACGCT GACCGTGCCG ACCGCGCGGG GCGATGCCGT TGTGCCGAAG 151 AATCCCGAAC GCGTCGCCGT GTACGACTGG GCGGCGTTGG ATACGCTGAC 201 CGAATTGGGC GTGAATGTGG GCGCAACCAC CGCGCCGGTG CGCGTGGATT 251 ATTTGCAGCC TGCATTTGAC AAGGCGGCAA CGGTGGGGAC GCTGTTCGAG 301 CCCGATTACG AAGCCCTGCA CCGCTACAAT CCTCAGCTTG TCATTACCGG 351 CGGGCCGGGC GCGGAAGCGT ATGAACAGTT AGCGAAAAAC GCGACCACCA 401 TAGATCTGAC GGTGGACAAC GGCAATATCC GCACCAGCGG CGAAAAGCAG 451 ATGGAGACCT TGGCGCGGAT TTTCGGCAAG GAAGCGCGCG CGGCGGAATT 501 GAAGGCGCAG ATTGACGCGC TGTTCGCCCA AACGCGCGAA GCCGCCAAAG 551 GCAAAGGACG CGGGCTGGTG CTGTCGGTTA CGGGCAACAA GGTGTCCGCC 601 TTCGGCACGC AGTCGCGGTT GGCAAGTTGG ATACACGGCG ACATCGGCCT 651 ACCGCCTGTA GACGAATCTT TACGCAACGA GGGGCACGGG CAGCCTGTTT 701 CCTTCGAATA CATCAAAGAG AAAAACCCCG ATTGGATTTT CATCATCGAC 751 CGTACCGCCG CCATCGGGCA GGAAGGGCCG GCGGCTGTCG AAGTATTGGA 801 TAACGCGCTG GTACGCGGCA CGAACGCTTG GAAGCGCAAG CAAATCATCG 851 TCATGCCTGC CGCGAACTAC ATTGTCGCGG GCGGCGCGCG GCAGTTGATT 901 CAGGCGGCGG AGCAGTTGAA GGCGGCGTTT AAAAAGGCAG AACCCGTTGC 951 GGCGGGGAAA AAGTAG This corresponds to the amino acid sequence <SEQ ID 2576; ORF 750>: m750.pep 1 VKPRFYWAAC AVLLTACSPE PAAEKTVSAA SAΞAATLTVP TARGDAVVPK 51 ΝPERVAVYDW AALDTLTELG VΝVGATTAPV RVDYLQPAFD KAATVGTLFE 101 PDYEALHRYΝ PQLVITGGPG AEAYEQLAKΝ ATTIDLTVDΝ GΝIRTSGEKQ 151 METLARIFGK EARAAELKAQ IDALFAQTRE AAKGKGRGLV LSVTGΝKVSA 201 FGTQSRLASW IHGDIGLPPV DESLRΝEGHG QPVSFEYIKE KΝPDWIFIID 251 RTAAIGQEGP AAVEVLDΝAL VRGTΝAWKRK QIIVMPAAΝY IVAGGARQLI 301 QAAEQLKAAF KKAEPVAAGK K* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N. gonorrhoeae ORF 750 shows 93.8% identity over a 322 aa overlap with a predicted ORF (ORF 750) from N gonorrhoeae m750/g750 93.8% identity in 322 aa overlap 10 20 30 40 50 m750.pep VKPRFYWAACAVLLTACSPEPAAEKTVSAASASA ATLTVPTARGDAWPKΝPERVA llllll M I II I I I I I I I I I I I I :| I I I II I I I I II I I I I I I I II I I g750 VKPRFYWAACAVLPAACSPEPAAEKTVSAASQAAΞTPVATLTVPTARGDAVVPKΝPERVA 10 20 30 40 50 60 60 70 80 90 100 110 m750.pep VYDWAALDTLTELGVΝVGATTAPVRVDYLQPAFDKAATVGTLFEPDYEALHRYΝPQLVIT I I II I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I III I I I I I I IM I Ml I Ml I I g750 VYDWAALDTLTEPGVΝVGATTAPVRVDYLQPAFDKAATVGTLFEPDCESLHRHΝPQFVIT 70 80 90 100 110 120 120 130 140 150 160 170 m750.pep GGPGAEAYE Q LAKΝATTIDLTVDΝGΝIRTSGEKQMETLARIFGKEARAAELKAQIDALFA I I Mil I I I I III I I I I I I I I I I I I II I II I I I I I I I |:| I || I I I I M I Ml I I I I I I I g750 GGPGAEAYEQLAKNATTIDLTVDNGNIRTΞGEKQMETLSRIFGKEARVAELNAQIDALFA 130 140 150 160 170 180 180 190 200 210 220 230 m750.pep QTREAAKGKGRGLVLΞVTGNKVΞAFGTQSRLAΞWIHGDIGLPPVDESLRNEGHGQPVSFE I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I g750 QKREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLPPVDEΞLRNEGHGQPVSFE 190 200 210 220 230 240 240 250 260 270 280 290 m750.pep YIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNAWKRKQIIVMPAANYIVAGGA I I I I I I I I II I I I I II I I I I I I I I I I I I I I I I I I I II I II I I I I I I I I I I I g750 YIKEKNPGWIFIIDRTAAIGQEGPAAVEVLDNALVCGTNAWKRKQIIVMPAANYIVAGGA 250 260 270 280 290 300 300 310 320 m750.pep RQLIQAAEQLKAAFKKAEPVAAGKKX I I I I I I I I I I I II I : I II I I I I g750 RQLIQAAEQLKAAFEKAEPVAAQX 310 320 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2577>: a750. seq 1 GTGAAACCGC GTTTTTATTG GGCAGCCTGC GCCGTCCTGC TGACCGCCTG 51 TTCGCCCGAA CCTGCCGCCG AAAAAACTGT ATCCGCCGCA TCCGCATCTG 101 CCGCCACACT GACCGTGCCG ACCGCGCGGG GCGATGCCGT TGTGCCGAAG 151 AATCCCGAAC GCGTCGCCGT GTACGACTGG GCGGCGTTGG ATACGCTGAC 201 CGAATTGGGT GTGAATGTGG GCGCAACCAC CGCGCCGGTG CGCGTGGATT 251 ATTTGCAGCC TGCATTTGAC AAGGCGGCAA CGGTGGGGAC GCTGTTCGAG 301 CCCGATTACG AAGCCCTGCA CCGCTACAAT CCTCAGCTTG TCATTACCGG 351 CGGGCCGGGC GCGGAAGCGT ATGAACAGTT GGCGAAAAAC GCGACCACCA 401 TAGATCTGAC GGTGGACAAC GGCAATATCC GCACCAGCGG CGAAAAGCAG 451 ATGGAGACCT TGGCGCGGAT TTTCGGCAAG GAAGCGCGCG CGGCGGAATT 501 GAAGGCGCAG ATTGACGCGC TGTTCGCCCA AACGCGCGAA GCCGCCAAAG 551 GCAAAGGACG CGGGCTGGTG CTGTCGGTTA CGGGCAACAA GGTGTCCGCC 601 TTCGGCACGC AGTCGCGGTT GGCAAGTTGG ATACACGGCG ACATCGGCCT 651 ACCGCCTGTA GACGAATCTT TACGCAACGA GGGGCACGGG CAGCCTGTTT 701 CCTTCGAATA CATCAAAGAG AAAAACCCCG ATTGGATTTT CATCATCGAC 751 CGTACCGCCG CCATCGGGCA GGAAGGGCCG GCGGCTGTCG AAGTATTGGA 801 TAACGCGCTG GTACGCGGCA CGAACGCTTG GAAGCGCAAG CAAATCATCG 851 TCATGCCTGC CGCGAACTAC ATTGTCGCGG GCGGCTCGCG GCAGTTGATT 901 CAGGCGGCGG AGCAGTTGAA GGAGGCGTTT GAAAAGGCAG AACCCGTTGC 951 GGCGGGGAAA GAGTAG This corresponds to the amino acid sequence <SEQ ID 2578; ORF 750.a>: a750.pep 1 VKPRFYWAAC AVLLTACSPE PAAEKTVSAA SAΞAATLTVP TARGDAWPK 51 ΝPERVAVYDW AALDTLTELG VΝVGATTAPV RVDYLQPAFD KAATVGTLFE 101 PDYEALHRYΝ PQLVITGGPG AEAYEQLAKΝ ATTIDLTVDΝ GΝIRTSGEKQ 151 METLARIFGK EARAAELKAQ IDALFAQTRE AAKGKGRGLV LSVTGΝKVSA 201 FGTQSRLASW IHGDIGLPPV DESLRΝEGHG QPVSFEYIKE KΝPDWIFIID 251 RTAAIGQEGP AAVEVLDΝAL VRGTΝAWKRK QIIVMPAAΝY IVAGGSRQLI 301 QAAEQLKEAF EKAEPVAAGK E* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 750 shows 98.8% identity over a 321 aa overlap with a predicted ORF (ORF 750) from N meningitidis: a750/m750 98.8% identity in 321 aa overlap 10 20 30 40 50 60 a750.pep VKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARGDAWPKΝPERVAVYDW I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m750 VKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARGDAWPKΝPERVAVYDW 10 20 30 40 50 60 70 80 90 100 110 120 a750.pep AALDTLTELGVΝVGATTAPVRVDYLQPAFDKAATVGTLFEPDYEALHRYΝPQLVITGGPG III II I II I I I I II I I I I II I II I I I I I I I I I I I I I I I I I I I I I II I II I II I I m750 AALDTLTELGVΝVGATTAPVRVDYL Q PAFDKAATVGTLFEPDYEALHRYΝPQLVITGGPG 70 80 90 100 110 120 130 140 150 160 170 180 a750. pep AEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGKEARAAELKAQIDALFAQTRE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m750 AEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGKEARAAELKAQIDALFAQTRE 130 140 150 160 170 180 190 200 210 220 230 240 a750. .pep AAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLPPVDEΞLRNEGHGQPVSFEYIKE I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I m750 AAKGKGRGLVLSVTGNKVSAFGTQΞRLASWIHGDIGLPPVDESLRNEGHGQPVSFEYIKE 190 200 210 220 230 240 250 260 270 280 290 300 a750. ■pep KNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNAWKRKQIIVMPAANYIVAGGSRQLI I I I II I II I II I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I II I I Ml I I I m750 KNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNAWKRKQIIVMPAANYIVAGGARQLI 250 260 270 280 290 300 310 320 a750. .pep QAAEQLKEAFEKAEPVAAGKEX I IM I I I I I I I I : I m750 QAAEQLKAAFKKAEPVAAGKKX 310 320 g751.seq not found yet g 7 51.pep not found yet The following partial DNA sequence was identified in N meningitidis <SEQ ID 2579>: m751.seq.. 1 ATGGCTTGGA GTATGTTTGC CACAACCCAA GCCGATAGAG CGGTAAGGTC 51 TGCAACTGCA CCTAAAGAAA TGTGGTTCCA TAAGAAGATA ATAGATGAAA 101 AAACAGGTAA AGTATCCTTT GATACCAGAC AAATTTGGTC ATTGAATGAT 151 TTAAGCAAGG AAGAACTGGC AAGCATTCAA GACACAAATG GCAAAGTTAT 201 TACTGTGTCT AATCCTGGTA TTTTCAATAA TCGAGAAGAT TCATTAAGCA 251 ACGCAGCAAA ACAAAATCGT AATAGTACAA ACGGTAGTGG TGTTATTGCA 301 GTCATGAATC CTCCAACAGG GAAATATAAA TCTGATTCTA ATAACAAAAT 351 AAAAGATTTT TTATGGCTCG GTTCAAGTCT TGTTTCTGAA CTGATGTATG 401 TCGGTTACGA CCAATTAAAT AATAAAGTGT TCCAAGGCTA TTTACCCAAA 451 ACCAATTCAG AAAAACTGAA TCAAGATATT TATCGAGAGG TTCAAAAAAT 501 GGGTAACGGC TGGTCGGTTG ATACCAGTAA TCACAGTCGT GGGGGAATTA 551 CAGCAAGCGT TTCCTTAAAA GATTGGGTAA ACAATCAAAA ACAAAATGGC 601 ATTGCCCCAA TCAGAAAAGC ACGTTTCTAT GGTACAGCCA CAAATGTGCA 651 GAATGATTAC GCCGATGTTT TACAGAAAAA CGGCTATACC TATACGGGTG 701 CAGACGGCAA AACTTATAAC AGCGGATCCT ACTCAATCGT GCATGATAAA 751 GATTTTGTGG GGAACAAATG GATACCTTTC TTGCTAGGAA CCAATGACAC 801 CACACAAGGT ACATGTAAGG GGTTGTGCTA TTCGCATAGC AGTTATTTTG 851 CGGAGGTGCC AAAAGCAGGT ACAAAAGAAT TTGATGACTA TGTAAAAATA 901 TGGGGTGAAG TTGAATATGA CGCTCAAGGT AAGCCAATTA ACAAATCTAA 951 ACCCATACTG GTAGAACCAA ACAAAACAAA AGATAATGAA AAATATGAAA 1001 AAGAAGCTTT CTAA This corresponds to the amino acid sequence <SEQ ID 2580; ORF 751>: m751.pep.. 1 MAWSMFATTQ ADRAVRSATA PKEMWFHKKI IDEKTGKVSF DTRQIWSLΝD 51 LSKEELASIQ DTΝGKVITVS ΝPGIFΝΝRED SLSΝAAKQΝR ΝSTΝGSGVIA 101 VMΝPPTGKYK SDSΝ KIKDF LWLGSSLVSE LMYVGYDQLΝ ΝKVFQGYLPK 151 TΝSEKLΝQDI YREVQKMGΝG WSVDTSΝHSR GGITASVSLK DWVΝΝQKQΝG 201 IAPIRKARFY GTATΝVQΝDY ADVLQKΝGYT YTGADGKTYΝ SGSYSIVHDK 251 DFVGΝKWIPF LLGTΝDTTQG TCKGLCYSHΞ SYFAEVPKAG TKEFDDYVKI 301 WGEVEYDAQG KPIΝKSKPIL VEPΝKTKDΝE KYEKEAF* a751.seq not found yet a751.pep not found yet g752.seq not found yet g752.pep not found yet The following partial DNA sequence was identified in N meningitidis <SEQ ID 2581>: m752.seq.. 1 ATGAAAATTT CCAGACCTCC GGAATTTACC CTGTTGCAAC AGGAATATAT 51 GCAGCATCTC ACTGAAAGAA TGACGCAAAT TGCCAAGCTG CTGAATTCTT 101 CCGCAAACAA TCCTGATATA GACATTCCCG ATTTTCTTAC TGAAATCAAA 151 GATTATTCAG AATTTTCCGT GACAGATGAA AATGGAACCT ACCTGCATTG 201 GGACAAATTC CGCCGGATTC ACACGGAAGA TACGCGGATG AAATGGCGCG 251 CCGTTAAGGA AAGCCGCAAA AAAATCCAAA AACCAATTGA TTTCCCGTTT 301 GAACATCAGT TTTGGTTCTG CATTCCCGAC TCTTTGCAGG CACGGCTTCA 351 TTTGATTGAC AAAAGCTGCG GCAGTTCTAT CGGCACGTCT AGCTTGGGTG 401 GCTTCGGCAG AAGCGAGCAA AACAGATTCT TGCTCAAGTC TCTGATTATG 451 GAAGAAGCGA TTACATCCGC CCAACTGGAA GGTGCGGCTA CCACGCGTAA 501 AGTGGCCAAG GATATGCTCA AATCGCAGCG TAAACCCAAA ACAAAAGACG 551 AAATCATGAT AGTGAACAAC TATCACTTGA TGAAAAAAGC GGTAGAATTG 601 AAAAATACGC CGTTAAGTGT TGAAATGATT TTGGATTTGC ACCGCATTGC 651 TACCAGTAAC GCTATTGAAA ACAAGGCCGA GCCCGGACAA TTCAGGCAGG 701 ATGACGAAAT CTTTATCGCC GATATCAATG GTAACAGCCT GTATCAACCA 751 CCGCCGCACG GACAGGTTCA TACGCTGATG GAAGAGGTGT GTGCGTTTGC 801 CAATAATACC TATGACGGCG TGGAAAATCC GTTTATCCAT CCGGTTGTCC 851 AAGCTATTAT CTTGCATTTC CTCATCGGCT ACATCCACCC ATTTGGTGAT 901 GGCAACGGGC GGACAGCGCG GGCTTTGTTC TATTGGTTTA TGCTCAAAAA 951 CGGCTACTGG CTATTTGAAT ACATATCCAT CAGCCGTCTT CTGAAAAACG 1001 CTCCTGCCCA ATACGCCAAA TCCTATTTGT ATGCGGAAAC TGACGATTTA 1051 GATTTAACCT ATTTCATCTA TTACCAATGC GATATTATCA AGCGGGCGGT 1101 TGCCGATTTG GAGCACTACA TTTCCGACAA ACAAAAACAC CAACAGGAAT 1151 TCAAAGCAGC GATTGCCCAA TATACTGAAA AGATAGGAAA GTTGAACCAA 1201 CGGCAAATTG GTATCCTGCA AAAAGCAGTG GAAGAAAGCG GAAAAATCTT 1251 TACTGCACAA GAAATTGCCA ACCAATACGG CATCTCCCTG AATACTGCCC 1301 GTAGCGATTT GAGTAAACTG GGAGAATATA GATTCCTAGT GCCGTTCAAA 1351 TCAGGAAATG CTTTAGAGTA TGTTGCTCCT CAGGATTTAT TGGAAAGGTT 1401 AGAAAAAAAA TAG This corresponds to the amino acid sequence <SEQ ID 2582; ORF 752>: m752.pep 1 MKISRPPEFT LLQQEY QHL TER TQIAKL LΝSSAΝΝPDI DIPDFLTEIK 51 DYSEFSVTDE ΝGTYLHWDKF RRIHTEDTRM KWRAVKESRK KIQKPIDFPF 101 EHQFWFCIPD SLQARLHLID KSCGSSIGTS SLGGFGRSEQ ΝRFLLKSLI 151 EEAITSAQLE GAATTRKVAK DMLKSQRKPK TKDEIMIVΝΝ YHL KKAVEL 201 KΝTPLSVEMI LDLHRIATSΝ AIEΝKAEPGQ FRQDDEIFIA DIΝGΝSLYQP 251 PPHGQVHTLM EEVCAFAΝΝT YDGVEΝPFIH PWQAIILHF LIGYIHPFGD 301 GΝGRTARALF YWFMLKΝGYW LFEYISISRL LKΝAPAQYAK SYLYAETDDL 351 DLTYFIYYQC DIIKRAVADL EHYISDKQKH QQEFKAAIAQ YTEKIGKLΝQ 401 RQIGILQKAV EESGKIFTAQ EIAΝQYGISL ΝTARSDLSKL GEYRFLVPFK 451 SGΝALEYVAP QDLLERLEKK * The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2583>: m752-l.seq 1 ATGAAAATTT CCAGACCTCC GGAATTTACC CTGTTGCAAC AGGAATATAT 51 GCAGCATCTC ACTGAAAGAA TGACGCAAAT TGCCAAGCTG CTGAATTCTT 101 CCGCAAACAA TCCTGATATA GACATTCCCG ATTTTCTTAC TGAAATCAAA 151 GATTATTCAG AATTTTCCGT GACAGATGAA AATGGAACCT ACCTGCATTG 201 GGACAAATTC CGCCGGATTC ACACGGAAGA TACGCGGATG AAATGGCGCG 251 CCGTTAAGGA AAGCCGCAAA AAAATCCAAA AACCAATTGA TTTCCCGTTT 301 GAACATCAGT TTTGGTTCTG CATTCCCGAC TCTTTGCAGG CACGGCTTCA 351 TTTGATTGAC AAAAGCTGCG GCAGTTCTAT CGGCACGTCT AGCTTGGGTG 401 GCTTCGGCAG AAGCGAGCAA AACAGATTCT TGCTCAAGTC TCTGATTATG 451 GAAGAAGCGA TTACATCCGC CCAACTGGAA GGTGCGGCTA CCACGCGTAA 501 AGTGGCCAAG GATATGCTCA AATCGCAGCG TAAACCCAAA ACAAAAGACG 551 AAATCATGAT AGTGAACAAC TATCACTTGA TGAAAAAAGC GGTAGAATTG 601 AAAAATACGC CGTTAAGTGT TGAAATGATT TTGGATTTGC ACCGCATTGC 651 TACCAGTAAC GCTATTGAAA ACAAGGCCGA GCCCGGACAA TTCAGGCAGG 701 ATGACGAAAT CTTTATCGCC GATATCAATG GTAACAGCCT GTATCAACCA 751 CCGCCGCACG GACAGGTTCA TACGCTGATG GAAGAGGTGT GTGCGTTTGC 801 CAATAATACC TATGACGGCG TGGAAAATCC GTTTATCCAT CCGGTTGTCC 851 AAGCTATTAT CTTGCATTTC CTCATCGGCT ACATCCACCC ATTTGGTGAT 901 GGCAACGGGC GGACAGCGCG GGCTTTGTTC TATTGGTTTA TGCTCAAAAA 951 CGGCTACTGG CTATTTGAAT ACATATCCAT CAGCCGTCTT CTGAAAAACG 1001 CTCCTGCCCA ATACGCCAAA TCCTATTTGT ATGCGGAAAC TGACGATTTA 1051 GATTTAACCT ATTTCATCTA TTACCAATGC GATATTATCA AGCGGGCGGT 1101 TGCCGATTTG GAGCACTACA TTTCCGACAA ACAAAAACAC CAACAGGAAT 1151 TCAAAGCAGC GATTGCCCAA TATACTGAAA AGATAGGAAA GTTGAACCAA 1201 CGGCAAATTG GTATCCTGCA AAAAGCAGTG GAAGAAAGCG GAAAAATCTT 1251 TACTGCACAA GAAATTGCCA ACCAATACGG CATCTCCCTG AATACTGCCC 1301 GTAGCGATTT GAGTAAACTG GGAGAATATA GATTCCTAGT GCCGTTCAAA 1351 TCAGGAAATG CTTTAGAGTA TGTTGCTCCT CAGGATTTAT TGGAAAGGTT 1401 AGAAAAAAAA TAG This corresponds to the amino acid sequence <SEQ ID 2584; ORF 752-l>: 752-l.pep 1 MKISRPPEFT LLQQEYMQHL TERMTQIAKL LNSSANNPDI DIPDFLTEIK 51 DYSEFΞVTDE NGTYLHWDKF RRIHTEDTRM KWRAVKEΞRK KIQKPIDFPF 101 EHQFWFCIPD SLQARLHLID KSCGSSIGTS SLGGFGRSEQ NRFLLKSLIM 151 EEAITSAQLE GAATTRKVAK DMLKSQRKPK TKDEIMIVNN YHLMKKAVEL 201 KNTPLSVEMI LDLHRIATSN AIENKAEPGQ FRQDDEIFIA DINGNΞLYQP 251 PPHGQVHTLM EEVCAFANNT YDGVENPFIH PWQAIILHF LIGYIHPFGD 301 GNGRTARALF YWFMLKNGYW LFEYISIΞRL LKNAPAQYAK SYLYAETDDL 351 DLTYFIYYQC DIIKRAVADL EHYISDKQKH QQEFKAAIAQ YTEKIGKLNQ 401 RQIGILQKAV EESGKIFTAQ EIANQYGISL NTARΞDLSKL GEYRFLVPFK 451 SGNALEYVAP QDLLERLEKK * a752.seq not found yet a752.pep not found yet g753.seq not found yet g753.pep not found yet The following partial DNA sequence was identified in N meningitidis <SEQ ID 2585>: m753.seq 1 ATGCCCATCA CTCCACCCTT AAACATCATC TCTCCTAAAC TCTACCCCAA 51 TGAACAATGG AACGAAAGCG AAGCACTCGG TGCCATCACT TGGCTATGGT 101 ATCAGTCGCC TACGCATCGC CAAGTACCTA TTGTGGAGAT GATGACGTAT 151 ATATTGCCTG TGTTAAAAAA CGGGCAGTTC GCTTTGTTTT GCAAGGGTAC 201 CCAACCAATC GGTTATATCT CATGGGCTTA TTTTGATGAA GTGGCGCAGG 251 CGCATTATTT AGAATCTGAC CGCCATTTGC GTGACAACAG CGATTGGAAC 301 TGTGGCGACA ATATTTGGCT GATTCAATGG TTTGCGCCAT TGGGACACAG 351 TCATCAAATG CGCTCAGCTG TGCGCCAGTT ATTTCCTAGT ACGACAGTAC 401 GCGCCTTGTA TCATAAAGGG AGCGATAAGG GTTTGAGAAT TTTAACTTTT 451 AAAACTTGA This corresponds to the amino acid sequence <SEQ ID 2586; ORF 753>: m753.pep 1 MPITPPLΝII SPKLYPΝEQW ΝESEALGAIT WLWYQSPTHR QVPIVE MTY 51 ILPVLK GQF ALFCKGTQPI GYISWAYFDE VAQAHYLESD RHLRDΝSDWΝ 101 CGDΝIWLIQW FAPLGHSHQM RSAVRQLFPS TTVRALYHKG SDKGLRILTF 151 KT* a753.seq not found yet a753.pep not found yet g754.seq not found yet g754 . pep not found yet The following partial DNA sequence was identified in N meningitidis <SEQ ID 2587>: m754.seq 1 ATGATGAAGT CTATCCTCAC CGTATCCGGA AATCGTATGC GTAAACCCAG 51 AATCACCTAT TTGGATGTTT GGGCAAACGA TGAAAGAATC GGTACTTTGG 101 AAAAGGGGGC CATGTATCGG TTCGCATACG ACAATCCCAA TTCTTCGTTG 151 CTGGGCCTGC ATTATCAAGA CAGAAGCAAG GTATATATCA GCAACAATAT 201 GCCGCATATC TTTGCACAGT ATTTTCCGGA AGGCTTTTTG GATGCACACA 251 TCACAAGCAA ATATGCTTTT CATGATGCGC CTTTTGAAGA CAATGAGATG 301 CTGCGCTTGG CAATTCTGTG CAGAGAGACT TTGGGTCGGA TACATGTGCG 351 CTGTAATGAC CCGCTTTTTA ATGAATGGAT TGACGGGTTG GAGATGAAAA 401 ATCCAAGAAT ATTGACTGAA CGGGATTTGC TGGGCATAAA TGCCCGACAG 451 GTTTTTCAGC AATATATGGC AGAAATCTTC CATCACGGCC GTTTCGTCAG 501 TGTATCCGGG ATACAGCAGA AGATGTCCTT AGATGCCATC CGCAGAAATA 551 CCAAGCAAAC TGCCTCATAT ATTGCCAAAG GTTTTGATGC ATCCGAATAT 601 CCTTGCTTGG CTGCCAATGA ATTTTTATGC ATGCAGACCA TCAAACAAGC 651 CGGCATTGCC GTTGCACAGA CCAGCCTGTC GGAAGATTCA TCAGTCTTAT 701 TGGTACGTCG GTTTGATGTC AGTGAACAGG GTTATTTTTT AGGGATGGAA 751 GACTTTACCA GTCTGCGCCA GTATTCGGTA GAAGATAAAT ATAAAGGCAG 801 TTATGCGGCT ATTGCACAGA TTATCCGACA GATATCCGGC AGACCAGATG 851 AAGATTTAAT CCATTTCTTT AATCAGCTTG CTGCCAGTTG CATATTGAAA 901 AACGGCGATG CACACCTCAA AAATTTTTCA GTACTCTATC ATGACGAATA 951 CGATGTTCGT CTTGCACCTG TCTATGATGT ATTGGATACA TCAATATACA 1001 GGGTTGGAAC ACAAGGAATT TTTGATGCTT ATGACGATAC GCTGGCATTA 1051 AACCTGACTA ACCACGGTAA GAAAACATAT CCTTCCAAGA ATACATTGTT 1101 GGATTTTGCT GAGAAATATT GCGATTTGGG AAGAGAAGAT GCATCCTTTA 1151 TGATAGATAC AATCGTTCAA GCTAAAGAAC AGGTTCTTGT TAAATACTCG 1201 GATGTATTGC GTGAGAATGA ATGGTTGGCG CAGAAGTGGC ATTTTATCCC 1251 GGATGAAAAT GAAGAAGGTC TACCGTTTAC ATTCCGGTAG This corresponds to the amino acid sequence <SEQ ID 2588; ORF 754>: m754.pep 1 MMKSILTVSG ΝRMRKPRITY LDVWAΝDERI GTLEKGAMYR FAYDΝPΝSSL 51 LGLHYQDRSK VYISΝΝMPHI FAQYFPEGFL DAHITSKYAF HDAPFEDΝEM 101 LRLAILCRET LGRIHVRCΝD PLFΝEWIDGL EMKΝPRILTE RDLLGIΝARQ 151 VFQQYMAEIF HHGRFVSVSG IQQK SLDAI RRΝTKQTASY IAKGFDASEY 201 PCLAAΝEFLC MQTIKQAGIA VAQTSLSEDS SVLLVRRFDV SEQGYFLG E 251 DFTSLRQYSV EDKYKGSYAA IAQIIRQISG RPDEDLIHFF ΝQLAASCILK 301 ΝGDAHLKΝFS VLYHDEYDVR LAPVYDVLDT SIYRVGTQGI FDAYDDTLAL 351 ΝLTΝHGKKTY PSKΝTLLDFA EKYCDLGRED ASFMIDTIVQ AKEQVLVKYS 401 DVLREΝEWLA QKWHFIPDEΝ EEGLPFTFR* a754.seq not found yet a754.pep not found yet g755.seq not found yet g755.pep not found yet The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 2589>: m755. seq.. 1 ATGAGCCGTT ACCTGATTAC CTTTGATATG GATACCAACT GCCTGAAAGA 51 CAATTACCAC GGAAATAACT ATACCAATGC CTACTCCGAT ATTAAAACCA 101 TCTTGGCTAG ACATGGATTT GAGAACATTC AGGGCAGTGT TTATCTAGGC 151 CGTGAAGGCA TCAGTGAAGC ACACGGAACA ATAGCCATTC AGGAACTGAC 201 CGCTCGGTTT GATTGGTTTT ACTCCTGTAT TTCAAACATT AAGTTTTACC 251 GCCTTGAAAG TGATTTGAAC GCACAATTTA TCGCTGATGG TGTGTATCAA 301 GCCAAACAGG CTTTCCTTCA ACGTGTTGAA CAACTTCGTA TATCCCTAAC 351 AGAAGCTGGA TTGTCTGATG AGCAAATCAA TCAGGTTCTG GAAAAACAGA 401 AATTTGAATT GGAAAGTCCT AACCTGAAAT TAAATTAA This corresponds to the amino acid sequence <SEQ ID 2590; ORF 755>: m755.pep.. 1 MSRYLITFDM DTNCLKDNYH GNNYTNAYSD IKTILARHGF ENIQGSVYLG 51 REGISEAHGT IAIQELTARF DWFYSCISNI KFYRLESDLN AQFIADGVYQ 101 AKQAFLQRVE QLRISLTEAG LSDEQINQVL EKQKFELESP NLKLN* a755.seq not found yet a755.pep not found yet g756.seq not found yet g756.pep not found yet The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2591 >: m756. seq 1 ATGACCGCCA ACTTTGCACA GACGCTGGTC GAAATACAGG ACAGTCTGTA 51 CAGGGTTGTG TCAACCGTCC AATACGGGGA TGACAACCTC AAGCGGTTGA 101 CAGCGGACAA ACGGAAGCAG TATGAGTTGA ACTTCAAGAT TTCCGAGGGT 151 TCTACGCGTG TAGAGTCCGA CTTTAAAGAG ACTTTGGTTC GGTTCGGTAG 201 AGATATGCTT CAAGATATGC CCCCTAAAAT CCGTTCGGCA ACGCTGGTAG 251 CGTTGACGAC CCTGCTTGTC GGAGGGGCGT TGGGTTACGG TTATTTGGAA 301 TACCTGAAGC AGGTTGCTTC GGAAGGGTAT CAGACCGAGC GTCTGTATAA 351 TGCCGTCGAC CGTCTTGCAG AATCCCAAGA ACGGATAACG TCCGCCATCC 401 TGAAGGGTGC TAGAGGTGCC GATTTCGTGC AAATCGGCAG ACGTTCCTAC 451 AGTAGGGAGG ATATATCGGA GGCAAATAGA CGTGCAGAGC GTGTCCCGTA 501 TGGCGCAGAG TTGGTTTCAG ACGGCAATTT TACCGCTGTT TTATCTGATA 551 TAGGGGATTA A This corresponds to the amino acid sequence <SEQ ID 2592; ORF 756>: m756.pep 1 MTAΝFAQTLV EIQDSLYRW STVQYGDDΝL KRLTADKRKQ YELΝFKIΞEG 51 STRVESDFKE TLVRFGRDML QDMPPKIRSA TLVALTTLLV GGALGYGYLE 101 YLKQVASEGY QTERLYΝAVD RLAESQERIT SAILKGARGA DFVQIGRRSY 151 SREDISEAΝR RAERVPYGAE LVSDGΝFTAV LSDIGD* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2593>: a756. seq 1 ATGACCGCCA ACTTTGCACA GACGCTGGTC GAAATACAGG ACAGTCTGTA 51 ΝAGGGTTGTG TCAACCGTCC AATACGGGGA TGACAACCTC AAGCGGTTGA 101 CAGCGGACAA ACGGAAGCAG TATGAGTTGA ACTTCAAGAT TTCCGAGGGT 151 TCTACGCGTG TAGAGTCCGA CTTTAAAGAG ACTTTGGTTC GGTTCGGTAG 201 AGATATGCTT CAAGATATGC CCCCTAAAAT CCGTTCGGCA ACGCTGGTAG 251 CGTTGACGAC CCTGCTTGTC GGAGGGGCGT TGGGTTACGG TTATTTGGAA 301 TACCTGAAGC AGGTTGCTTC GGAAGGGTAT CAGACCGAGC GTCTGTATAA 351 TGCCGTCGAC CGTCTTGCAG AATCCCAAGA ACGGATAACG TCCGCCATCC 401 TGAAGGGTGC TAGAGGTGCC GATTTCGTGC AAATCGGCAG ACGTTCCTAC 451 AGTAGGGAGG ATATATCGGA GGCAAATAGA CGTGCAGAGC GTGTCCCGTA 501 TGGCGCAGAG TTGGTTTCAG ACGGCAATTT TACCGCTGTT TTATCTGATA 551 TAGGGGATTA A This corresponds to the amino acid sequence <SEQ ID 2594; ORF 756. a>: a756.pep 1 MTAΝFAQTLV EIQDSLXRVV STVQYGDDΝL KRLTADKRKQ YELΝFKISEG 51 STRVESDFKE TLVRFGRDML QDMPPKIRSA TLVALTTLLV GGALGYGYLE 101 YLKQVASEGY QTERLYΝAVD RLAESQERIT SAILKGARGA DFVQIGRRSY 151 SREDISEAΝR RAERVPYGAE LVSDGΝFTAV LSDIGD* m756 / a756 99.5% identity in 186 aa overlap 10 20 30 40 50 60 m756.pep MTAΝFAQTLVEIQDSLYRVVSTVQYGDDΝLKRLTADKRKQYELΝFKISEGSTRVESDFKE I I I I I I I I M M II II II II II II II II II I I I I I I I II I II II II II II II II I I I I I a756 MTAΝFA Q TLVEI Q DSLXRVVSTVQYGDDΝLKRLTADKRKQYELΝFKISEGSTRVESDFKE 10 20 30 40 50 60 70 80 90 100 110 120 m756. pep TLVRFGRDMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYLKQVASEGYQTERLYNAVD II II I II II II I II I II II II I II I II II II II I II I II II II I I II II I I II I II I II I a756 TLVRFGRDMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYLKQVASEGYQTERLYNAVD 70 80 90 100 110 120 130 140 150 160 170 180 m756.pep RLAESQERITSAILKGARGADFVQIGRRSYSREDISEANRRAERVPYGAELVSDGNFTAV MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a756 RLAESQERITSAILKGARGADFVQIGRRSYSREDISEANRRAERVPYGAELVSDGNFTAV 130 140 150 160 170 180 m756.pep LSDIGDX I II I II I a756 LSDIGDX g757.seq not found yet g757.pep not fiund yet The following partial DNA sequence was identified in N meningitidis <SEQ ID 2595>: m757. seq 1 ATGAAAATAC TCGCTTTATT AATTGCCGCT ACCTGTGCTT TATCTGCGTG 51 TGGCAGCCAA TCTGAAGAAC AACCGGCATC TGCACAACCC CAAGAGCAGG 101 CACAATCCGA ATTAAAAACC ATGCCGGTAA GCTATACCGA CTATCAATCA 151 GCAGCCAATA AAGGGCTGAA TGACCAAAAA ACCGGTCTGA CCCTTCCTGA 201 ACATGTTGTC CCTATCGACA ATGCGGAAGG AAAGAATCTG CTGCATGACT 251 TTTCAGACGG CCTCACAATC TTAACCGTTG ATACCGATAA AGCCGACAAA 301 ATTACTGCTG TCCGAGTAGT CTGGAATACA GATGCAATGC CTCAAAAAGC 351 GGAAAAACTG TCCAAAGCTG CCGCAGCCTT GATTGCGGCA ACCGCTCCGG 401 AAGACCGCAC AATGCTGCGT GATACCGGCG ACCAAATCGA AATGGCGATT 451 GACAGCCATA ATGCGCAAAA AGAGCCAACC CGAGAATGGG CGCGTGGTGG 501 GATTGCTTAT AAAGTCACTG TTACCAATTT ACCGAGCGTG GTTTTGACGG 551 CAAAAGCTGA GTAA This corresponds to the amino acid sequence <SEQ ID 2596; ORF 757>: m757.pep (lipoprotein) 1 MKILALLIAA TCALSACGSQ SEEQPASAQP QEQAQSELKT MPVΞYTDYQS 51 AAΝKGLΝDQK TGLTLPEHW PIDΝAEGKΝL LHDFΞDGLTI LTVDTDKADK 101 ITAVRVVWΝT DAMPQKAEKL ΞKAAAALIAA TAPEDRTMLR DTGDQIEMAI 151 DSHΝAQKEPT REWARGGIAY KVTVTΝLPSV VLTAKAE* a757.seq not found yet a757.pep not found yet g758.seq not found yet g758.pep not fiund yet The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2597>: m758. seq 1 ATGAACAATC TGACCGTGTT TACCCGTTTC GATACCGATT TGGCGACGCT 51 TGCCGATGAA TTGCAATATG TGTGGGAACA CACCGCCGTT ACAGACCATC 101 AGGGCAAACT GGTGGAAATT CCCGTCTGCT ACGGCGGCGA ATACGGCCCG 151 GATTTGGCGG AAGTCGCTGC TTTCCATCAG ACGGTTATTT CCGAAATCGT 201 CCGCCGCCAT ACGGCGCAAA CTTATACCGT ATTTATGATG GGCTTCCAGC 251 CCGGTTTCCC TTATCTGGGC GGCTTGCCCG AAGCATTGCA CACGCCCCGC 301 CGTGCCGTGC CGAGAACGTC CGTTCCTGCC GGTTCGGTCG GTATCGGCGG 351 CAGTCAGACC GGTGTGTATC CGTTCGCTTC GCCCGGCGGC TGGCAGATTA 401 TCGGCAGAAC CGAATTACCC TTGTTCCGAG CCGATTTGAA TCCGCCGACC 451 CTGCTGGCGG CGGGTGACCA AGTCCGCTTT GTTGCAGAAA GGATTGAGCC 501 ATGA This corresponds to the amino acid sequence <SEQ ID 2598; ORF 758>: m758.pep 1 MNNLTVFTRF DTDLATLADE LQYVWEHTAV TDHQGKLVEI PVCYGGEYGP 51 DLAEVAAFHQ TVISEIVRRH TAQTYTVFMM GFQPGFPYLG GLPEALHTPR 101 RAVPRTSVPA GSVGIGGSQT GVYPFASPGG WQIIGRTELP LFRADLNPPT 151 LLAAGDQVRF VAERIEP* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2599>: a758. seq 1 ATGAACAATC TGACCGTGTT CACCCGTTTC GATACCGATT TGGCGACGCT 51 TGCCGATGAA TTGCAATATG TGTGGGAACA CACCGCCGTT ACAGACCATC 101 AGGGCAAACT GGTGGAAATT CCCGTCTGCT ACGGCGGCGA ATACGGCCCG 151 GATTTGGCGG AAGTCGCTGC TTTCCATCAG ACGGTTATTT CCGAAATCGT 201 CCGCCGCCAT ACGGCGCAAA CTTATACCGT ATTTATGATG GGCTTCCAGC 251 CTGGTTTCCC TTATCTGGGC GGCTTGCCCG AAGCATTGCA CACGCCCCGC 301 CGTGCCGTGC CGAGAACGTC CGTTCCTGCC GGTTCGGTCG GTATCGGCGG 351 CAGTCAGACC GGTGTGTATC CGTTCGCTTC GCCCGGCGGC TGGCAGATTA 401 TCGGCAGAAC CGAATTACCC TTGTTCCGAG CCGATTTGAA TCCGCCGACC 451 CTGCTGGCGG CGGGTGACCA AGTCCGCTTT GTTGCAGAAA GGATTGAGCC 501 ATGA This corresponds to the amino acid sequence <SEQ ID 2600; ORF 758. a>: a758.pep.. 1 MΝΝLTVFTRF DTDLATLADE LQYVWEHTAV TDHQGKLVEI PVCYGGEYGP 51 DLAEVAAFHQ TVISEIVRRH TAQTYTVFMM GFQPGFPYLG GLPEALHTPR 101 RAVPRTSVPA GSVGIGGSQT GVYPFASPGG WQIIGRTELP LFRADLNPPT 151 LLAAGDQVRF VAERIEP* m758 / a758 100.0% identity in 167 aa overlap 10 20 30 40 50 60 m758.pep MNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAEVAAFHQ II I II II I II II II II II II II II II II II II II I I II II II II I I I I I I I II II II I I I a758 MNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAEVAAFHQ 10 20 30 40 50 60 70 80 90 100 110 120 m758. pep TVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGSVGIGGSQT MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a758 TVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGSVGIGGSQT 70 80 90 100 110 120 130 140 150 160 m758.pep GVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEPX I II I II II II II II II II II II II II II I II II II I I I II II I I I II I a758 GVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEPX 130 140 150 160 g759.seq not found yet g759.pep not found yet The following partial DNA sequence was identified in N meningitidis <SEQ ID 2601>: m759. seq 1 ATGCGCTTCA CACACACCAC CCCATTTTGT TCCGTATTGT CCACCCTCGG 51 TCTTTTTGCC GTTTCCCCTG CTTACTCATC CATTGTCCGC AACGATGTCG 101 ATTACCAATA TTTTCGCGAC TTTGCCGAAA ATAAAGGCGC GTTCACCGTA 151 GGTGCAAGCA ATATTTCCAT CCAAGACAAG CAAGGCAAAA TATTAGGCAG 201 GGTTCTCAAC GGCATCCCCA TGCCCGACTT CCGCGTCAGC AACCGCCAAA 251 CCGCCATCGC CACCCTGGTT CACCCCCAAT ACGTCAACAG TGTCAAACAC 301 AACGTCGGCT ACGGTTCCAT ACAATTCGGC AACGACACCC AAAATCCAGA 351 AGAACAAGCC TATACCTACC GCCTCGTATC ACGCAACCCG CACCCGGACT 401 ACGACTACCA CCTTCCCCGC CTCAACAAAC TGGTTACCGA AATCTCACCT 451 ACCGCACTCA GCAGCGTACC CTTGCTTGGA AACGGCCAGC CAAAGGCCAA 501 TGCCTACCTC GATACCGACC GCTTCCCCTA CTTTGTACGA CTCGGCTCAG 551 GCACGCAACA AGTCCGCAAA GCAGACGGCA CGCGTACACG AACCGCCCCG 601 GCATACCAAT ACCTGACCGG CGGCACGCCG CTGAAAGTAT TGGGGTTCCA 651 AAACCACGGC TTACTCGTCG GCGGCAGCCT GACCGACCAA CCCCTTAACA 701 CCTACGCAAT CGCCGGAGAC AGCGGTTCCC CCCTGTTTGC CTTCGACAAG 751 CATGAAAACC GCTGGGTGCT TGCGGGCGTA CTCAGCACCT ACGCCGGCTT 801 CGATAATTTC TTCAACAAAT ACATCGTCAC GCAACCCGAA TTCATCCGTT 851 CCACCATCCG CCAATACGAA ACCCGGCTGG ATGTCGGGCT GACCACCAAC 901 GAACTCATAT GGCGCGACAA CGGTAATGGC AACAGCACCC TGCAAGGGCT 951 CAACGAACGC ATCACCCTGC CCATTGCAAA CCCTTCGCTT GCCCCACAAA 1001 ACGACAGCAG GCACATGCCG TCTGAAGATG CCGGCAAAAC GCTCATCCTA 1051 TCCAGCAGGT TCGACAACAA AACACTGATG CTGGCAGACA ATATCAACCA 1101 AGGCGCAGGC GCATTGCAGT TCGACAGCAA CTTCACCGTC GTCGGTAAAA 1151 ACCACACATG GCAAGGTGCA GGCGTTATCG TAGCCGACGG CAAACGCGTC 1201 TTCTGGCAAG TCAGCAACCC CAAAGGCGAC CGGCTCTCCA AACTGGGCGC 1251 AGGCACGCTT ATCGCCAACG GACAAGGCAT CAACCAGGGC GACATCAGCA 1301 TCGGGGAAGG CACTGTCGTA CTCGCCCAAA AAGCTGCTTC AGACGGCAGC 1351 AAACAAGCAT TCAACCAAGT CGGCATCACC AGCGGCAGGG GCACGGCCGT 1401 CCTCGCCGAC AGCCAGCAAA TCAAACCCGA AAACCTCTAT TTCGGCTTCA 1451 GGGGCGGACG GCTCGACCTC AACGGCAACA ACCTTGCCTT TACCCATATC 1501 CGCCATGCGG ACGGCGGCGC GCAAATCGTC AATCACAACC CTGACCAAGC 1551 CGCGACACTG ACGCTGACCG GCAACCCCGT CCTCAGTCCC GAGCATGTCG 1601 AGTGGGTGCA ATGGGGCAAC CGTCCGCAAG GCAACGCGGC GGTTTACGAA 1651 TACATCAACC CGCACCGCAA CCGTCGGACC GACTACTTCA TACTCAAACC 1701 CGGCGGCAAC CCGCGCGAAT TTTTCCCGTT AAATATGAAA AACTCAACAA 1751 GCTGGCAATT TATCGGCAAC AACAGGCAAC AGGCCGCCGA ACAAGTCGCC 1801 CAAGCCGAAA ATGCCCGCCC CGACCTGATT ACCTTCGGCG GATACTTGGG 1851 TGAAAACGCG CAAACGGGCA AAGCCGCGCC GAGTTACAGC AAAACCAATG 1901 AAGCAGCCAT AGAAAAAACC CGCCATATCG CAAATGCCGC CGTATACGGC 1951 CGGCCCGAAT ACCGTTACAA CGGCGCACTC AACCTGCACT ATCGTCCCAA 2001 ACGCACCGAC AGCACGCTGT TGCTCAACGG CGGCATGAAC CTTAACGGGG 2051 AAGTCTTGAT TGAGGGCGGC AATATGATTG TGTCAGGCAG GCCCGTACCC 2101 CATGCCTACG ACCACCAGGC CAAACGCGAA CCCGTTCTTG AAAACGAATG 2151 GACCGACGGC AGCTTCAAGG CTGCACGGTT CACCCTGCGA AACCATGCCC 2201 GACTGACGGC AGGGCGCAAT ACCGCGCATC TGGACGGCGA CATAACCGCA 2251 TACGATCTGT CCGGCATCGA CCTCGGCTTT ACCCAAGGCA AAACACCGGA 2301 ATGCTACCGC TCCTACCATA GCGGCAGCAC CCACTGCACA CCCAACGCCG 2351 TTTTAAAAGC CGAAAACTAT CGTGCACTAC CTGCAACGCA AGTACGCGGC 2401 GACATTACCC TTAACGACCG TTCAGAGCTC CGCCTGGGCA AAGCACACCT 2451 GTACGGCAGC ATCCGTGCCG GCAAAGACAC CGCAGTCCGC ATGGAAGCAG 2501 ACAGCAACTG GACACTTTCC CAGTCCAGCC ACACCGGCGC ACTGACGCTT 2551 GACGGCGCAC AAATTACCCT GAACCCCGAT TTCGCCAATA ATACACACAA 2601 CAACCGCTTC AACACACTGA CCGTCAACGG CACACTTGAC GGGTTCGGCA 2651 CATTCCGATT CCTGACCGGC ATCGTCCGAA AACAAAATGC CCCCCCCCTC 2701 AAACTGGAAG GGGACAGCCG CGGCGCATTC CAAATCCACG TCAAAAACAC 2751 CGGACAAGAA CCTCAAACAA CCGAATCGCT TGCACTTGTG AGCCTCAATC 2801 CGAAACACAG CCACCAAGCC CGATTCACCC TCCAAAACGG CTATGCCGAT 2851 TTGGGTGCCT ACCGCTACAT CCTCCGCAAA AACAACAACG GATACAGCCT 2901 GTACAACCCG CTCAAAGAGG CCGAACTTCA AATTGAAGCC ACGCGTGCGG 2951 AACATGAGCG CAACCAACAG GCATACAACC AATTACAGGC AACCGACATC 3001 AGCAGACAGG TTCAACATGA CTCTGACGCG ACCAGGCAGG CACTACAGGC 3051 CTGGCAGAAC AGTCAAACCG AACTTGCCCG CATCGACAGC CAAGTCCAAT 3101 ATCTGTCCGC CCAATTGAAA CAGACAGACC CGCTGACCGG CATTCTGACG 3151 CGTGCCCAAA ACCTGTGTGC CGCACAAGGA TACAGTGCCG ATATCTGCCG 3201 TCAGGTTGCC AAAGCCGCCG ACACGAACGA CCTGACACTC TTCGAAACCG 3251 AACTGGATAC GTATATAGAA CGTGTAGAAA TGGCCGAATC CGAACTTGAC 3301 AAAGCACGGC AAGGCGGCGA TGCGCAAGCC GTCGAAACAG CCCGGCACGC 3351 CTACCTGAAC GCACTCAACC GTCTGTCCCG ACAAATCCAC AGTTTGAAAA 3401 CCGGCGTTGC CGGCATCCGT ATGCCGAACC TGGCCGAACT GATCAGCCGG 3451 TCGGCCAACA CCGCCGTTTC CGAACAGGCC GCCTACAATA CCGGCCGGCA 3501 ACAGGCGGGA CGCCGCATCG ACCGCCACCT TACCGATCCG CAGCAGCAAA 3551 ACATCTGGCT GGAAACCGGT ACGCAACAAA CCGACTACCA TAGCGGCACA 3601 CACCGTCCCT ACCAACAAAC TACCAACTAT GCACATATCG GCATCCAAAC 3651 CGGCATCACC GACCGTCTCA GTGTCGGTAC GATTTTAACC GATGAGCGCA 3701 CAAACAACCG TTTTGATGAA GGCGTATCCG CCCGAAACCG CAGCAACGGC 3751 GCACATCTGT TCGTCAAAGG GGAAAACGGC GCACTCTTTG CCGCGGCAGA 3801 TTTAGGCTAC AGCAACAGCC GTACCCGATT TACCGATTAT GACGGGGCTG 3851 CCGTCCGCCG CCACGCATGG GATGCAGGCA TCAACACCGG CATCAAAATC 3901 GATACCGGCA TCAACCTCAG ACCCTATGCC GGCATCCGTA TAAACCGCAG 3951 CAACGGCAAC CGGTACGTAC TCGACGGCGC AGAGATAAAC AGCCCGGCGC 4001 AAATCCAAAC CACATGGCAT GCCGGCATCC GTCTCGATAA AACCGTCGAA 4051 CTGGGTCAAG CCAAGCTGAC CCCCGCCTTC AGCAGCGATT ACTACCATAC 4101 CCGCCAAAAC AGCGGTTCCG CCCTCAGCGT CAACGACCGT ACCTTACTGC 4151 AGCAAGCCGC CCACGGCACA CTGCATACCC TGCAAATCGA CGCCGGATAC 4201 AAAGGCTGGA ACGCCAAACT TCATGCCGCT TACGGCAAAG ACAGCAACAC 4251 CGCCCGCCAC AAACAGGCAG GAATCAAAAT AGGCTACAAC TGGTAA This corresponds to the amino acid sequence <SEQ ID 2602; ORF 759>: m759.pep 1 MRFTHTTPFC SVLSTLGLFA VSPAYSSIVR NDVDYQYFRD FAENKGAFTV 51 GASNISIQDK QGKILGRVLN GIPMPDFRVS NRQTAIATLV HPQYVNSVKH 101 NVGYGSIQFG NDTQNPEEQA YTYRLVSRNP HPDYDYHLPR LNKLVTEISP 151 TALSSVPLLG NGQPKANAYL DTDRFPYFVR LGSGTQQVRK ADGTRTRTAP 201 AYQYLTGGTP LKVLGFQNHG LLVGGSLTDQ PLNTYAIAGD SGSPLFAFDK 251 HENRWVLAGV LSTYAGFDNF FNKYIVTQPE FIRSTIRQYE TRLDVGLTTN 301 ELIWRDNGNG NSTLQGLNER ITLPIANPSL APQNDSRHMP SEDAGKTLIL 351 SSRFDNKTLM LADNINQGAG ALQFDSNFTV VGKNHTWQGA GVIVADGKRV 401 FWQVSNPKGD RLSKLGAGTL IANGQGINQG DISIGEGTVV LAQKAASDGS 451 KQAFNQVGIT SSGGRRGGTTAAVVLLAADD SQQIKPENLY FGFRGGRLDL NGNNLAFTHI 501 RHADGGAQIV NNHHNNPPDDQQAAAATTLL TLTGNPVLSP EHVEWVQWGN RPQGNAAVYE 551 YINPHRNRRT DDYYFFIILLKKPPGGGGNN PREFFPLNMK NSTSWQFIGN NRQQAAEQVA 601 QAENARPDLI TFGGYLGENA QTGKAAPSYS KTNEAAIEKT RHIANAAVYG 651 RPEYRYNGAL NLHYRPKRTD STLLLNGGMN LNGEVLIEGG NMIVSGRPVP 701 HAYDHQAKRE P PVVLLEENNEEWWTTDDGG SFKAARFTLR NHARLTAGRN TAHLDGDITA 751 YDLSGIDLGF TQGKTPECYR SYHSGSTHCT PNAVLKAENY RALPATQVRG 801 DITLNDRSEL RLGKAHLYGS IRAGKDTAVR MEADSNWTLS QSSHTGALTL 851 DGAQITLNPD FANNTHNNRF NTLTVNGTLD GFGTFRFLTG IVRKQNAPPL 901 KLEGDSRGAF QIHVKNTGQE PQTTESLALV SLNPKHSHQA RFTLQNGYAD 951 LGAYRYILRK NNNGYSLYNP LKEAELQIEA TRAEHERNQQ AYNQLQATDI 1001 SRQVQHDSDA TRQALQAWQN SQTELARIDS QVQYLSAQLK QTDPLTGILT 1051 RAQNLCAAQG YSADICRQVA KAADTNDLTL FETELDTYIE RVEMAESELD 1101 KARQGGDAQA VETARHAYLN ALNRLSRQIH SLKTGVAGIR MPNLAELISR 1151 SANTAVSEQA AYNTGRQQAG RRIDRHLTDP QQQNIWLETG TQQTDYHSGT 1201 HRPYQQTTNY AHIGIQTGIT DRLSVGTILT DERTNNRFDE GVSARNRSNG 1251 AHLFVKGENG ALFAAADLGY SNSRTRFTDY DGAAVRRHAW DAGINTGIKI 1301 DTGINLRPYA GIRINRSNGN RYVLDGAEIN SPAQIQTTWH AGIRLDKTVE 1351 LGQAKLTPAF SSDYYHTRQN SGSALSVNDR TLLQQAAHGT LHTLQIDAGY 1401 KGWNAKLHAA YGKDSNTARH KQAGIKIGYN W* The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2603>: g760. seq (partial) 1 AACAACCGCA ACACCCGTTA CGCCGCATTG GGCAAACGCG TGATGGAAGG 51 CGTTGAGACC GAAATCAGCG GTGCGATTAC ACCGAAATGG CAAATCCATG 101 CAGGTTACAG CTATCTGCAC AGCCAAATCA AAACCGCCGC CAATCCACGC 151 GACGACGGCA TCTTCCTGCT GGTGCCCAAA CACAGCGCAA ACCTGTGGAC 201 GACTTACCAA GTTACGCCCG GGCTGACCGT CGGCGGCGGC GTGAACGCGA 251 TGAGCGGCAT TACTTCATCT GCAGGGATGC ATGCAGGCGG TTATGCCACG 301 TTCGATGCGA TGGCGGCATA CCGCTTCACG CCCAAGCTGA AGCTGCAAAT 351 CAATGCCGAC AACATCTTCA ACCGCCATTA CTACGCCCGC GTCGGCGGCA 401 CGAACACCTT TAACATTCCC GGTTCGGAGC GCAGCCTGAC GGCAAACCTG 451 CGTTACAGTT TTTAA This corresponds to the amino acid sequence <SEQ ID 2604; ORF 760.ng>: g760.pep (partial) 1 ΝΝRΝTRYAAL GKRVMEGVET EISGAITPKW QIHAGYSYLH SQIKTAAΝPR 51 DDGIFLLVPK HSAΝLWTTYQ VTPGLTVGGG VΝAMSGITSS AGMHAGGYAT 101 FDAMAAYRFT PKLKLQIΝAD ΝIFΝRHYYAR VGGTΝTFΝIP GSERSLTAΝL 151 RYSF* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2605>: m760. seq 1 ATGGGACAGT TTATGTCAGT TTTCCGCATC AATATGACCG CCGCCACGGT 51 TTTGGCAGCA CTCTCGTCTT CGGTTTTTGC CGCACAAACG GAAGGTTTGG 101 AAACCGTCCA TATTAAGGGT CAGCGTTCTT ACAACGCGAT TGCCACCGAG 151 AAAAACGGCG ATTACAGCTC GTTTGCCGCC ACCGTCGGTA CAAAAATCCC 201 CGCTTCTTTG CGCGAAATTC CGCAATCCGT CAGCATCATT ACCAACCAGC 251 AGGTCAAAGA CCGCAATGTT GATACGTTTG ACCAGTTGGC ACGCAAAACG 301 CCCGGCCTGC GCGTGTTGAG CAACGACGAC GGACGCTCTT CGGTTTACGC 351 GCGCGGTTAC GAATACAGCG AATACAACAT CGACGGCCTG CCCGCGCAGA 401 TGCAGAGTAT CAACGGCACG CTGCCCAACC TGTTCGCCTT CGACCGCGTG 451 GAAGTGATGC GCGGGCCGAG CGGACTGTTC GACAGCAGCG GCGAGATGGG 501 CGGCATCGTG AATCTGGTGC GCAAACGCCC GACCAAAGCG TTCCAAGGTC 551 ATGCGGCGGC AGGGTTCGGT ACGCACAAAC AATATAAAGC CGAGGCGGAC 601 GTATCGGGCA GCCTCAATTC AGACGGCAGC GTGCGCGGCC GCGTGATGGC 651 GCAGACCGTC GGCGCGTCTC CGCGTCCCGC CGAGAAAAAC AACCGGCGCG 701 AAACCTTCTA CGCGGCGGCG GATTGGGACA TCAACCCCGA TACGGTTTTG 751 GGCGCGGGCT ATCTTTACCA GCAACGCCGC CTCGCGCCGT ACAACGGCCT 801 GCCTGCCGAT GCCAATAACA AATTACCGTC CCTGCCGCAA CACGTATTTG 851 TCGGCGCGGA TTGGAACAAA TTTAAAATGC ACAGCCACGA CGTGTTCGCC 901 GATTTGAAAC ATTACTTCGG CAACGGCGGC TACGGCAAAG TCGGTATGCG 951 CTATTCCGAT CGGAAAGCCG ATTCCAATTA TACGTTTGCG GGCAGCAAAC 1001 TCAACAATAC CGGACAAGCC GACGTAGCGG GTTTGGGTAC GGACATTAAA 1051 CAAAAAGCCT TTGCGGTTGA CGCAAGTTAC AGCCGTCCGT TTGCCTTGGG 1101 CAACACCGCC AACGAATTTG TGATTGGTGC AGACTACAAC CGCTTGCGCA 1151 GTACTAATGA ACAAGGGCGT TCGACTTTGT CAAAAAGCGT CGCTTTAGAT 1201 GGTTTCCGCG CTTTGCCTTA TAACGGCATA CTTCAGAACG CCCGCGCCGG 1251 AAACAAAGGT TTCAATCACT CCGTTACCGA AGAAAACCTC GACGAAACCG 1301 GTTTGTATGC CAAGACGGTG TTCCGTCCTC TGGAAGGTTT GTCGTTGATT 1351 GCAGGCGGAC GTGTAGGACA TCACAAAATC GAGTCGGGCG ACGGCAAAAC 1401 CCTGCATAAA GCTTCGAAAA CCAAATTTAC AAGCTACGCC GGCGCGGTTT 1451 ACGATATAGA CGGCAGCAAC AGCCTGTACG CTTCCGCCTC CCAACTCTAC 1501 ACACCGCAAA CCAGCATCGG CACCGACGGC AAGCTGCTCA AACCGCGCGA 1551 AGGCAACCAG TTTGAAATCG GCTACAAAGG CAGCTACATG GACGACCGCC 1601 TCAATACCCG GGTTTCGTTC TACCGCATGA AGGATAAAAA CGCCGCCGCA 1651 CCGCTGGACT CAAACAACAA AAAAACCCGT TACGCCGCAT TGGGCAAACG 1701 CGTGATGGAA GGTGTTGAGA CCGAAATCAG CGGCGCGATG ACACCGAAAT 1751 GGCAAATCCA TGCAGGTTAC AGCTACCTGC ACAGCCAAAT CAAAACCGCC 1801 TCCAATTCGC GCGACGAAGG CATCTTCCTG CTGATGCCCA AACACAGCGC 1851 AAACCTGTGG ACGACTTACC AAGTTACGTC CGGGCTGACC ATCGGCGGCG 1901 GCGTGAACGC GATGAGCGGC ATTACTTCAT CTGCAGGGAT ACATGCAGGC 1951 GGTTATGCCA CGTTCGATGC GATGGCGGCA TACCGCTTCA CGCCCAAACT 2001 GAAGCTGCAA ATCAACGCCG ACAACATCTT CAACCGCCAT TACTACGCCC 2051 GCGTCGGCAG CGAGAGCACC TTTAACATTC CCGGTTCGGA GCGCAGCCTG 2101 ACGGCAAACC TGCGTTACAG TTTTTAA This corresponds to the amino acid sequence <SEQ ID 2606; ORF 760>: m760.pep 1 MGQFMSVFRI ΝMTAATVLAA LSSSVFAAQT EGLETVHIKG QRSYΝAIATE 51 KΝGDYSSFAA TVGTKIPASL REIPQSVSII TΝQQVKDRΝV DTFDQLARKT 101 PGLRVLSΝDD GRSSVYARGY EYSEYΝIDGL PAQMQSIΝGT LPΝLFAFDRV 151 EVMRGPSGLF DSSGEMGGIV ΝLVRKRPTKA FQGHAAAGFG THKQYKAEAD 201 VSGSLΝSDGS VRGRVMAQTV GASPRPAEKΝ ΝRRETFYAAA DWDIΝPDTVL 251 GAGYLYQQRR LAPYΝGLPAD AΝΝKLPSLPQ HVFVGADWΝK FKMHSHDVFA 301 DLKHYFGΝGG YGKVGMRYSD RKADSΝYTFA GSKLΝΝTGQA DVAGLGTDIK 351 QKAFAVDASY SRPFALGΝTA ΝEFVIGADYΝ RLRSTΝEQGR STLSKSVALD 401 GFRALPYΝGI LQΝARAGΝKG FΝHSVTEEΝL DETGLYAKTV FRPLEGLSLI 451 AGGRVGHHKI ESGDGKTLHK ASKTKFTSYA GAVYDIDGSΝ SLYASASQLY 501 TPQTSIGTDG KLLKPREGΝQ FEIGYKGSYM DDRLΝTRVSF YRMKDKΝAAA 551 PLDSΝΝKKTR YAALGKRVME GVETEISGAM TPKWQIHAGY SYLHSQIKTA 601 SΝSRDEGIFL LMPKHSAΝLW TTYQVTSGLT IGGGVΝAMSG ITSSAGIHAG 651 GYATFDAMAA YRFTPKLKLQ IΝADΝIFΝRH YYARVGSEST FΝIPGSERSL 701 TAΝLRYSF* m760 / g760 91.6% identity in 154 aa overlap 530 540 550 560 570 580 m760. pep YKGSYMDDRLNTRVSFYRMKDKNAAAPLDSNNKKTRYAALGKRVMEGVETEISGAMTPKW II : : I I I II II I I I I I I II I I I I I I : I I II g760 NNRNTRYAALGKRVMEGVETEISGAITPKW 10 20 30 590 600 610 620 630 640 m760.pep QIHAGYSYLHSQIKTASNSRDEGIFLLMPKHSANLWTTYQVTSGLTIGGGVNAMSGITSS II II I II II I II II II : I I I : II I I I : I II I II II II II II II I : I I II I I I I II II I g760 QIHAGYSYLHSQIKTAANPRDDGIFLLVPKHSANLWTTYQVTPGLTVGGGVNAMSGITSS 40 50 60 70 80 90 650 660 670 680 690 700 m760.pep AGIHAGGYATFDAMAAYRFTPKLKLQINADNIFNRHYYARVGSESTFNIPGSERSLTANL I I : II II II I II II I I II I II I I II II II I II I II II II II I : : I I I II II II II II II g760 AGMHAGGYATFDAMAAYRFTPKLKLQINADNIFNRHYYARVGGTNTFNIPGSERSLTANL 100 110 120 130 140 150 709 m760.pep RYSFX II I II g760 RYSFX g761.seq not found yet g761.pep not found yet The following partial DNA sequence was identified in N meningitidis <SEQ ID 2607>: m761. seq 1 ATGAAAATAT CATTTCATTT AGCTTTATTA CCCACGCTGA TTATTGCTTC 51 CTTCCCTGTT GCTGCCGCCG ATACGCAGGA CAATGGTGAA CATTACACCG 101 CCACTCTGCC CACCGTTTCC GTGGTCGGAC AGTCCGACAC CAGCGTACTC 151 AAAGGCTACA TCAACTACGA CGAAGCCGCC GTTACCCGCA ACGGACAGCT 201 CATCAAAGAA ACGCCGCAAA CCATCGATAC GCTCAATATC CAGAAAAACA 251 AAAATTACGG TACGAACGAT TTGAGTTCCA TCCTCGAAGG CAATGCCGGC 301 ATCGACGCTG CCTACGATAT GCGCGGTGAA AGCATTTTCC TGCGCGGTTT 351 TCAAGCCGAC GCATCCGATA TTTACCGCGA CGGCGTGCGC GAAAGCGGAC 401 AAGTGCGCCG CAGTACTGCC AACATCGAGC GCGTGGAAAT CCTGAAAGGC 451 CCGTCTTCCG TGCTTTACGG CCGCACCAAC GGCGGCGGCG TCATCAACAT 501 GGTCAGCAAA TACGCCAACT TCAAACAAAG CCGCAACATC GGAGCGGTTT 551 ACGGCTCATG GGCAAACCGC AGCCTGAATA TGGACATTAA CGAAGTGCTG 601 AACAAAAACG TCGCCATCCG TCTCACCGGC GAAGTCGGGC GCGCCAATTC 651 GTTCCGCAGC GGCATAGACA GCAAAAATGT CATGGTTTCG CCCAGCATTA 701 CCGTCAAACT CGACAACGGC TTGAAGTGGA CGGGGCAATA CACCTACGAC 751 AATGTGGAGC GCACGCCCGA CCGCAGTCCG ACCAAGTCCG TGTACGACCG 801 CTTCGGACTG CCTTACCGCA TGGGGTTCGC CCACCGGAAC GATTTTGTCA 851 AAGACAAGCT GCAAGTTTGG CGTTCCGACC TTGAATACGC CTTCAACGAC 901 AAATGGCGTG CCCAATGGCA GCTCGCCCAC CGCACGGCGG CGCAGGATTT 951 TGATCATTTC TATGCAGGCA GCGAAAATGG CAACTTAATC AAACGTAACT 1001 ACGCCTGGCA GCAGACCGAC AACAAAACCC TGTCGTCCAA CTTAACGCTC 1051 AACGGCGACT ACACCATCGG CCGTTTTGAA AACCACCTGA CCGTAGGCAT 1101 GGATTACAGC CGCGAACACC GCAACCCGAC ATTGGGTTTC AGCAGCGCCT 1151 TTTCCGCCTC CATCAACCCC TACGACCGCG CAAGCTGGCC GGCTTCGGGC 1201 AGATTGCAGC CTATTCTGAC CCAAAACCGC CACAAAGCCG ACTCCTACGG 1251 CATCTTTGTG CAAAACATCT TCTCCGCCAC GCCCGATTTG AAATTCGTCC 1301 TCGGCGGCCG TTACGACAAA TACACCTTTA ATTCCGAAAA CAAACTCACC 1351 GGCAGCAGCC GCCAATACAG CGGACACTCG TTCAGCCCCA ACATCGGCGC 1401 AGTGTGGAAC ATCAATCCCG TCCACACACT TTACGCCTCG TATAACAAAG 1451 GCTTCGCGCC TTATGGCGGA CGCGGCGGCT ATTTGAGCAT CGATACGTTG 1501 TCTTCCGCCG TGTTCAACGC CGACCCCGAG TACACCCGCC AATACGAAAC 1551 CGGCGTGAAA AGCAGTTGGC TGGACGACCG CCTCAGCACT ACGTTGTCTG 1601 CCTACCAAAT CGAACGCTTC AATATCCGCT ACCGCCCCGA TCCAAAAAAC 1651 AACCCTTATA TTTATGCGGT TAGCGGCAAA CACCGTTCGC GCGGCGTGGA 1701 ATTGTCCGCC ATCGGGCAAA TCATCCCCAA AAAACTCTAT CTGCGCGGTT 1751 CGTTGGGCGT GATGCAGGCG AAAGTCGTTG AAGACAAAGA AAATCCCGAC 1801 CGAGTGGGCA TCCATTTGAA TAATACCAGC AACGTTACCG GCAACCTGTT 1851 TTTCCGTTAT ACCCCGACCG AAAACCTCTA CGGCGAAATC GGCGTAACCG 1901 GTACAGGCAA ACGCTACGGT TACAACTCAA GAAATAAAGA AGTGACTACG 1951 CTTCCAGGCT TTGCCCGAGT TGATGCCATG CTTGGCTGGA ACCATAAAAA 2001 TGTTAACGTT ACCTTTGCCG CAGCCAATCT GCTCAATCAA AAATATTGGC 2051 GTTCGGACTC TATGCCGGGT AATCCGCGCG GCTATACTGC CCGGGTAAAT 2101 TACCGTTTCT GA This corresponds to the amino acid sequence <SEQ ID 2608; ORF 761>: m761.pep 1 MKISFHLALL PTLIIASFPV AAADTQDNGE HYTATLPTVS VVGQSDTSVL 51 KGYINYDEAA VTRNGQLIKE TPQTIDTLNI QKNKNYGTND LSSILEGNAG 101 IDAAYDMRGE SIFLRGFQAD ASDIYRDGVR ESGQVRRSTA NIERVEILKG 151 PSSVLYGRTN GGGVINMVSK YANFKQSRNI GAVYGSWANR SLNMDINEVL 201 NKNVAIRLTG EVGRANSFRS GIDSKNVMVS PSITVKLDNG LKWTGQYTYD 251 NVERTPDRSP TKSVYDRFGL PYRMGFAHRN DFVKDKLQVW RSDLEYAFND 301 KWRAQWQLAH RTAAQDFDHF YAGSENGNLI KRNYAWQQTD NKTLSSNLTL 351 NGDYTIGRFE NHLTVGMDYS REHRNPTLGF SSAFSASINP YDRASWPASG 401 RLQPILTQNR HKADSYGIFV QNIFSATPDL KFVLGGRYDK YTFNSENKLT 451 GSSRQYSGHS FSPNIGAVWN INPVHTLYAS YNKGFAPYGG RGGYLSIDTL 501 SSAVFNADPE YTRQYETGVK SSWLDDRLST TLSAYQIERF NIRYRPDPKN 551 NPYIYAVSGK HRSRGVELSA IGQIIPKKLY LRGSLGVMQA KVVEDKENPD 601 RVGIHLNNTS NVTGNLFFRY TPTENLYGEI GVTGTGKRYG YNSRNKEVTT 651 LPGFARVDAM LGWNHKNVNV TFAAANLLNQ KYWRSDSMPG NPRGYTARVN 701 YRF* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2609>: a761. seq 1 ATGAAAATAT CATTTCATTT AGCTTTATTA CCCACGCTGA TTATTGCTTC 51 CTTCCCTGTT GCTGCCGCCG ATACGCAGGA CAATGGTGAA CATTACACCG 101 CCACTCTGCC CACCGTTTCC GTGGTCGGAC AGTCCGACAC CAGCGTACTC 151 AAAGGCTACA TCAACTACGA CGAAGCCGCC GTTACCCGCA ACGGACAGCT 201 CATCAAAGAA ACGCCGCAAA CCATCGATAC GCTCAATATC CAGAAAAACA 251 AAAATTACGG CACGAACGAT TTGAGTTCCA TCCTCGAAGG CAATGCCGGC 301 ATCGACGCCG CCTACGATAT GCGCGGCGAA AGCATTTTCC TGCGCGGCTT 351 TCAAGCCGAC GCATCTGATA TTTACCGCGA CGGCGTACGC GAAAGCGGGC 401 AGGTGCGCCG TAGCACCGCC AACATCGAGC GCGTGGAAAT CCTGAAAGGT 451 CCGTCCTCCG TGCTTTATGG GCGTACCAAC GGCGGCGGTG TCATCAACAT 501 GGTCAGCAAA TACGCCAACT TCAAACAAAG CCGTAA ATC GGTACGGTTT 551 ATGGTTCGTG GGCAAACCGC AGCCTGAATA TGGACATCAA CGAAGTGCTG 601 AACAAAAACG TCGCCATCCG TCTCACCGGC GAAGTCGGGC GCGCCAATTC 651 GTTCCGCAGC GGCATAGACA GCAAAAATGT CATGGTTTCG CCCAGCATTA 701 CCGTCAAACT CGACAACGGC TTGAAGTGGA CGGGGCAATA CACCTACGAC 751 AATGTGGAGC GCACGCCCGA CCGCAGTCCG ACCAAGTCCG TGTACGACCG 801 CTTCGGACTG CCTTACCGCA TGGGGTTCGC CCACCGGAAC GATTTTGTCA 851 AAGACAAGCT GCAAGTTTGG CGTTCCGACC TTGAATACGC CTTCAACGAC 901 AAATGGCGTG CCCAATGGCA GCTCGCCCAC CGCACGGCGG CGCAGGATTT 951 TGATCATTTC TATGCAGGCA GCGAAAATGG CAACTTAATC AAACGTAACT 1001 ACGCCTGGCA GCAGACCGAC AACAAAACCC TGTCGTCCAA CTTAACGCTC 1051 AACGGCGACT ACACCATCGG CCGTTTTGAA AACCACCTGA CCGTAGGCAT 1101 GGATTACAGC CGCGAACACC GCAACCCGAC ATTGGGTTTC AGCAGCGCCT 1151 TTTCCGCCTC CATCAACCCC TACGACCGCG CAAGCTGGCC GGCTTCGGGC 1201 AGATTGCAGC CTATTCTGAC CCAAAACCGC CACAAAGCCG ACTCCTACGG 1251 CATCTTTGTG CAAAACATCT TCTCCGCCAC GCCCGATTTG AAATTCGTCC 1301 TCGGCGGCCG TTACGACAAA TACACCTTTA ATTCCGAAAA CAAACTCACC 1351 GGCAGCAGCC GCCAATACAG CGGACACTCG TTCAGCCCCA ACATCGGCGC 1401 AGTGTGGAAC ATCAATCCCG TCCACACACT TTACGCCTCG TATAACAAAG 1451 GCTTCGCGCC TTATGGCGGA CGCGGCGGCT ATTTGAGCAT CGATACGTTG 1501 TCTTCCGCCG TGTTCAACGC CGACCCCGAG TACACCCGCC AATACGAAAC 1551 CGGCGTGAAA AGCAGTTGGC TGGACGACCG CCTCAGCACT ACGTTGTCTG 1601 CCTACCAAAT CGAACGCTTC AATATCCGCT ACCGCCCCGA TCCAAAAAAC 1651 AACCCTTATA TTTATGCGGT TAGCGGCAAA CACCGTTCGC GCGGCGTGGA 1701 ATTGTCCGCC ATCGGGCAAA TCATCCCCAA AAAACTCTAT CTGCGCGGTT 1751 CGTTGGGCGT GATGCAGGCG AAAGTCGTTG AAGACAAAGA AAATCCCGAC 1801 CGAGTGGGCA TCCATTTGAA TAACACCAGC AACGTTACCG GCAACCTGTT 1851 TTTCCGTTAT ACCCCGACCG AAAACCTCTA CGGCGAAATC GGCGTAACCG 1901 GTACAGGCAA ACGCTACGGT TACGACTCAA GAAATAAAGA AGTGACTACG 1951 CTTCCAGGCT TTGCCCGAGT TGATGCCATG CTTGGCTGGA ACCATAAAAA 2001 TGTTAACGTT ACCTTTGCCG CAGCCAATCT GTTCAATCAA AAATATTGGC 2051 GTTCGGACTC TATGCCGGGT AATCCGCGCG GCTATACTGC CCGGGTAAAT 2101 TACCGTTTCT GA This corresponds to the amino acid sequence <SEQ ID 2610; ORF 761. a>: a761.pep 1 MKISFHLALL PTLIIASFPV AAADTQDNGE HYTATLPTVS VVGQSDTSVL 51 KGYINYDEAA VTRNGQLIKE TPQTIDTLNI QKNKNYGTND LSSILEGNAG 101 IDAAYDMRGE SIFLRGFQAD ASDIYRDGVR ESGQVRRSTA NIERVEILKG 151 PSSVLYGRTN GGGVINMVSK YANFKQSRNI GTVYGSWANR SLNMDINEVL 201 NKNVAIRLTG EVGRANSFRS GIDSKNVMVS PSITVKLDNG LKWTGQYTYD 251 NVERTPDRSP TKSVYDRFGL PYRMGFAHRN DFVKDKLQVW RSDLEYAFND 301 KWRAQWQLAH RTAAQDFDHF YAGSENGNLI KRNYAWQQTD NKTLSSNLTL 351 NGDYTIGRFE NHLTVGMDYS REHRNPTLGF SSAFSASINP YDRASWPASG 401 RLQPILTQNR HKADSYGIFV QNIFSATPDL KFVLGGRYDK YTFNSENKLT 451 GSSRQYSGHS FSPNIGAVWN INPVHTLYAS YNKGFAPYGG RGGYLSIDTL 501 SSAVFNADPE YTRQYETGVK SSWLDDRLST TLSAYQIERF NIRYRPDPKN 551 NPYIYAVSGK HRSRGVELSA IGQIIPKKLY LRGSLGVMQA KVVEDKENPD 601 RVGIHLNNTS NVTGNLFFRY TPTENLYGEI GVTGTGKRYG YDSRNKEVTT 651 LPGFARVDAM LGWNHKNVNV TFAAANLFNQ KYWRSDSMPG NPRGYTARVN 701 YRF* m761 / a761 99.6% identity in 703 aa overlap 10 20 30 40 50 60 m761.pep MKISFHLALLPTLIIASFPVAAADTQDNGEHYTATLPTVSVVGQSDTSVLKGYINYDEAA I I II I I II I II II II II I II I II II I II II II I II I I II II II I II II I I II II I I I I I I a761 MKISFHLALLPTLIIASFPVAAADTQDNGEHYTATLPTVSVVGQSDTSVLKGYINYDEAA 10 20 30 40 50 60 70 80 90 100 110 120 m761. pep VTRNGQLIKETPQTIDTLNIQKNKNYGTNDLSSILEGNAGIDAAYDMRGESIFLRGFQAD I I II I II II I II II II II I II II II II II II II II I II I II I II II II I II II II II II I a761 VTRNGQLIKETPQTIDTLNIQKNKNYGTNDLSSILEGNAGIDAAYDMRGESIFLRGFQAD 70 80 90 100 110 120 130 140 150 160 170 180 m761. pep ASDIYRDGVRESGQVRRSTANIERVEILKGPSSVLYGRTNGGGVINMVSKYANFKQSRNI I II II II I I II II II I I II II I II II II II II II I I II I I I I II II II II II II II I I I I a761 ASDIYRDGVRESGQVRRSTANIERVEILKGPSSVLYGRTNGGGVINMVSKYANFKQSRNI 130 140 150 160 170 180 190 200 210 220 230 240 m761. pep GAVYGSWANRSLNMDINEVLNKNVAIRLTGEVGRANSFRSGIDSKNVMVSPSITVKLDNG M II I II II II II I II II II II II II II II II II II II I II II I II II II I II II II I I I a761 GTVYGSWANRSLNMDINEVLNKNVAIRLTGEVGRANSFRSGIDSKNVMVSPSITVKLDNG 190 200 210 220 230 240 250 260 270 280 290 300 m761. pep LKWTGQYTYDNVERTPDRSPTKSVYDRFGLPYRMGFAHRNDFVKDKLQVWRSDLEYAFND II I II II II I II II II II II I II I I I I I I II I II II I II II I I I I II II I I II I II I I I I a761 LKWTGQYTYDNVERTPDRSPTKSVYDRFGLPYRMGFAHRNDFVKDKLQVWRSDLEYAFND 250 260 270 280 290 300 310 320 330 340 350 360 m761. pep KWRAQWQLAHRTAAQDFDHFYAGSENGNLIKRNYAWQQTDNKTLSSNLTLNGDYTIGRFE II I I I I II II II II II II II II II II I I I II I II I I II II I I I I I II II I II I I II I I I I a761 KWRAQWQLAHRTAAQDFDHFYAGSENGNLIKRNYAWQQTDNKTLSSNLTLNGDYTIGRFE 310 320 330 340 350 360 370 380 390 400 410 420 m761. pep NHLTVGMDYSREHRNPTLGFSSAFSASINPYDRASWPASGRLQPILTQNRHKADSYGIFV II I II I II II II II I II II II II I II I I II I I I II II II II II II II II I I I II I I II II a761 NHLTVGMDYSREHRNPTLGFSSAFSASINPYDRASWPASGRLQPILTQNRHKADSYGIFV 370 380 390 400 410 420 430 440 450 460 470 480 m761. pep QNIFSATPDLKFVLGGRYDKYTFNSENKLTGSSRQYSGHSFSPNIGAVWNINPVHTLYAS I II II I II II II II II II II II II II II II II I II I II II II I I I II II I II I II II II I a761 QNIFSATPDLKFVLGGRYDKYTFNSENKLTGSSRQYSGHSFSPNIGAVWNINPVHTLYAS 430 440 450 460 470 480 490 500 510 520 530 540 m761. pep YNKGFAPYGGRGGYLSIDTLSSAVFNADPEYTRQYETGVKSSWLDDRLSTTLSAYQIERF I II II II II II II II I II II II II I II II II I II I II I I II I II I II I I I I II II II II I a761 YNKGFAPYGGRGGYLSIDTLSSAVFNADPEYTRQYETGVKSSWLDDRLSTTLSAYQIERF 490 500 510 520 530 540 550 560 570 580 590 600 m761.pep NIRYRPDPKNNPYIYAVSGKHRSRGVELSAIGQIIPKKLYLRGSLGVMQAKVVEDKENPD MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a761 NIRYRPDPKNNPYIYAVSGKHRSRGVELSAIGQIIPKKLYLRGSLGVMQAKVVEDKENPD 550 560 570 580 590 600 610 620 630 640 650 660 m761. pep RVGIHLNNTSNVTGNLFFRYTPTENLYGEIGVTGTGKRYGYNSRNKEVTTLPGFARVDAM II II II II II I I II I I II II II II II I I II I II II I II II I : I II II II I II I I II I I I I a761 RVGIHLNNTSNVTGNLFFRYTPTENLYGEIGVTGTGKRYGYDSRNKEVTTLPGFARVDAM 610 620 630 640 650 660 670 680 690 700 m761. pep LGWNHKNVNVTFAAANLLNQKYWRSDSMPGNPRGYTARVNYRFX I II II I I I II I I II II M II II II II I II II II II II I II II II a761 LGWNHKNVNVTFAAANLFNQKYWRSDSMPGNPRGYTARVNYRFX 670 680 690 700 g762.seq Not yet found g762.pep Not yet found The following partial DNA sequence was identified in N meningitidis <SEQ ID 2611>: m762.seq 1 ATGAAGTGGT TATTAAATAT GATAATGAGA CCTATTAAAT TTAGTATGGT 51 AAATACGTTA TTATTTATTG TTATATGTAG TTCATTTTTT GATCTGCTCG 101 TTCAATTATG TACAATTTTA TTTCATAGCC AAAAAATATA CTTTATTACA 151 TTATTTTTAT TATTTATTTT TAATTTTGTT ACAAAATCTA TCTATATGGC 201 AATTATTTAT CCTATTTTAT ATTTTTTTAC GATAAAAAAA TATTATCCTT 251 ACTCTAGGAA AGTGATAATT CTATTATCAT TAGCATTATC TATATATTTT 301 AGTTTTATGG ACTTTTACTT TTTTTCCATA TATTCAGATA ACCTTAGCTA 351 TGAAACGGAG CCTTTACATT TATACATCCC TATTATTATT AATTTTTTCT 401 CACTTTTAGT TTCTAATTTT ATTTTATCTT TTATCAACAA GTAA This corresponds to the amino acid sequence <SEQ ID 2612; ORF 762>: m762.pep 1 MKWLLΝMIMR PIKFSMVΝTL LFIVICSSFF DLLVQLCTIL FHSQKIYFIT 51 LFLLFIFΝFV TKSIYMAIIY PILYFFTIKK YYPYSRKVII LLSLALSIYF 101 SFMDFYFFSI YSDΝLSYETE PLHLYIPIII ΝFFSLLVSΝF ILSFIΝK* The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 2613>: a762.seq 1 ATGAAGTGGT TATTAAATAT GATAATGAGA CCTATTAAAT TTAGTATGGT 51 AAATACGTTA TTATTTATTG TTATATGTAG TTCATTTTTT GATCTGCTCG 101 TTCAATTATG TACAATTTTA TTTCATAGCC AAAAAATATA CTTTATTACA 151 TTATTTTTAT TATTTATTTT TAATTTTGTT ACAAAATCTA TCTATATGGC 201 AATTATTTAT CCTATTTTAT ATTTTTTTAC GATAAAAAAA TATTATCCTT 251 ACTCTAGGAA AGTGATAATT CTATTATCAT TAGCATTATC TATATATTTT 301 AGTTTTATGG ACTTTTACTT TTTTTCCATA TATTCAGATA ACCTTAGCTA 351 TGAAACGGAG CCTTTACATT TATACATCCC TATTATTATT AATTTTTTCT 401 CACTTTTAGT TTCTAATTTT ATTTTATCTT TTATCAACAA GTAA This corresponds to the amino acid sequence <SEQ ID 2614; ORF 762. a>: a762.pep 1 MKWLLNMIMR PIKFSMVNTL LFIVICSSFF DLLVQLCTIL FHSQKIYFIT 51 LFLLFIFNFV TKSIYMAIIY PILYFFTIKK YYPYSRKVII LLSLALSIYF 101 SFMDFYFFSI YSDNLSYETE PLHLYIPIII NFFSLLVSNF ILSFINK* m762 / a762 100.0% identity in 147 aa overlap 10 20 30 40 50 60 m762.pep MKWLLNMIMRPIKFSMVNTLLFIVICSSFFDLLVQLCTILFHSQKIYFITLFLLFIFNFV MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a762 MKWLLNMIMRPIKFSMVNTLLFIVICSSFFDLLVQLCTILFHSQKIYFITLFLLFIFNFV 10 20 30 40 50 60 70 80 90 100 110 120 m762.pep TKSIYMAIIYPILYFFTIKKYYPYSRKVIILLSLALSIYFSFMDFYFFSIYSDNLSYETE I II II I II I I II II II II II II II I II I II I I II II I II II II I II II II II I II I I I II a762 TKSIYMAIIYPILYFFTIKKYYPYSRKVIILLSLALSIYFSFMDFYFFSIYSDNLΞYETE 70 80 90 100 110 120 130 140 m762.pep PLHLYIPIIINFFSLLVSNFILSFINKX II I I I II I II II II II II II II II II II a762 PLHLYIPIIINFFSLLVSNFILSFINKX 130 140 g763.seq not yet found g763.pep not yet found The following partial DNA sequence was identified in N meningitidis <SEQ ID 2615>: m763. seq 1 ATGACATTGC TCAATCTAAT GATAATGCAA GATTACGGTA TTTCCGTTTG 51 CCTGACACTG ACGCCCTATT TGCAACATGA ACTATTTTCG GCTATGAAAT 101 CCTATTTTTC CAAATATATC CTACCCGTTT CACTTTTTAC CTTGCCACTA 151 TCCCTTTCCC CATCCGTTTC GGCTTTTACG CTGCCTGAAG CATGGCGGGC 201 GGCGCAGCAA CATTCGGCTG ATTTTCAAGC GTCCCATTAC CAGCGTGATG 251 CAGTGCGCGC ACGGCAACAA CAAGCCAAGG CCGCATTCCT TCCCCATGTA 301 TCCGCCAATG CCAGCTACCA GCGCCAGCCG CCATCGATTT CTTCCACCCG 351 CGAAACACAG GGATGGAGCG TGCAGGTGGG ACAAACCTTA TTTGACGCTG 401 CCAAATTTGC ACAATACCGC CAAAGCAGGT TCGATACGCA GGCTGCAGAA 451 CAGCGTTTCG ATGCGGCACG CGAAGAATTG CTGTTGAAAG TTGCCGAAAG 501 TTATTTCAAC GTTTTACTCA GCCGAGACAC CGTTGCCGCC CATGCGGCGG 551 AAAAAGAGGC TTATGCCCAG CAGGTAAGGC AGGCGCAGGC TTTATTCAAT 601 AAAGGTGCTG CCACCGCGCT GGATATTCAC GAAGCCAAAG CCGGTTACGA 651 CAATGCCCTG GCCCAAGAAA TCGCCGTATT GGCTGAGAAA CAAACCTATG 701 AAAACCAGTT GAACGACTAC ACCGACCTGG ATAGCAAACA AATCGAGGCC 751 ATAGATACCG CCAACCTGTT GGCACGCTAT CTGCCCAAGC TGGAACGTTA 801 CAGTCTGGAT GAATGGCAGC GCATTGCCTT ATCCAACAAT CATGAATACC 851 GGATGCAGCA GCTTGCCCTG CAAAGCAGCG GACAGGCGCT TCGGGCAGCA 901 CAGAACAGCC GCTATCCCAC CGTTTCTGCC CATGTCGGCT ATCAG ATAA 951 CCTCTACACT TCATCTGCGC AGAATAATGA CTACCACTAT CGGGGCAAAG 1001 GGATGAGCGT CGGCGTACAG TTGAATTTGC CGCTTTATAC CGGCGGAGAA 1051 TTGTCGGGCA AAATCCATGA AGCCGAAGCG CAATACGGGG CCGCCGAAGC 1101 ACAGCTGACC GCAACCGAGC GGCACATCAA ACTCGCCGTA CGCCAGGCTT 1151 ATACCGAAAG CGGTGCGGCG CGTTACCAAA TCATGGCGCA AGAACGGGTT 1201 TTGGAAAGCA GCCGTTTGAA ACTGAAATCG ACCGAAACCG GCCAACAATA 1251 CGGCATCCGC AACCGGCTGG AAGTAATACG GGCGCGGCAG GAAGTCGCCC 1301 AAGCAGAACA GAAACTGGCT CAAGCACGGT ATAAATTCAT GCTGGCTTAT 1351 TTGCGCTTGG TGAAAGAGAG CGGGTTAGGG TTGGAAACGG TATTTGCGGA 1401 ATAA This corresponds to the amino acid sequence <SEQ ID 2616; ORF 763>: m763.pep 1 MTLLNLMIMQ DYGISVCLTL TPYLQHELFS AMKSYFSKYI LPVSLFTLPL 51 SLSPSVSAFT LPEAWRAAQQ HSADFQASHY QRDAVRARQQ QAKAAFLPHV 101 SANASYQRQP PSISSTRETQ GWSVQVGQTL FDAAKFAQYR QSRFDTQAAE 151 QRFDAAREEL LLKVAESYFN VLLSRDTVAA HAAEKEAYAQ QVRQAQALFN 201 KGAATALDIH EAKAGYDNAL AQEIAVLAEK QTYENQLNDY TDLDSKQIEA 251 IDTANLLARY LPKLERYSLD EWQRIALSNN HEYRMQQLAL QSΞGQALRAA 301 QNSRYPTVSA HVGYQNNLYT SSAQNNDYHY RGKGMSVGVQ LNLPLYTGGE 351 LSGKIHEAEA QYGAAEAQLT ATERHIKLAV RQAYTESGAA RYQIMAQERV 401 LESSRLKLKS TETGQQYGIR NRLEVIRARQ EVAQAEQKLA QARYKFMLAY 451 LRLVKESGLG LETVFAE* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2617>: a763.seq 1 ATGACATTGC TCAATCTAAT GATAATGCAA GATTACGGTA TTTCCGTTTG 51 CCTGACACTG ACGCCCTATT TGCAACATGA ACTATTTTCG GCTATGAAAT 101 CCTATTTTTC CAAATATATC CTACCCGTTT CACTTTTTAC CTTGCCACTA 151 TCCCTTTCCC CATCCGTTTC GGCTTTTACG CTGCCTGAAG CATGGCGGGC 201 GGCGCAGCAA CATTCGGCTG ATTTTCAAGC GTCCCATTAC CAGCGTGATG 251 CAGTGCGCGC ACGGCAACAA CAAGCCAAGG CCGCATTCCT TCCCCATGTA 301 TCCGCCAATG CCAGCTACCA GCGCCAGCCG CCATCGATTT CTTCCACCCG 351 CGAAACACAG GGATGGAGCG TGCAGGTGGG ACAAACCTTA TTTGACGCTG 401 CCAAATTTGC ACAATACCGC CAAAGCAGGT TCGATACGCA GGCTGCAGAA 451 CAGCGTTTCG ATGCGGCACG CGAAGAATTG CTGTTGAAAG TTGCCGAAAG 501 TTATTTCAAC GTTTTACTCA GCCGAGACAC CGTTGCCGCC CATGCGGCGG 551 AAAAAGAGGC TTATGCCCAG CAGGTAAGGC AGGCGCAGGC TTTATTCAAT 601 AAAGGTGCTG CCACCGCGCT GGATATTCAC GAAGCCAAAG CCGGTTACGA 651 CAATGCCCTG GCCCAAGAAA TCGCCGTATT GGCTGAGAAA CAAACCTATG 701 AAAACCAGTT GAACGACTAC ACCGGCCTGG ACAGCAAACA AATCGAGGCC 751 ATAGATACCG CCAACCTGTT GGCACGCTAT CTGCCCAAGC TGGAACGTTA 801 CAGTCTGGAT GAATGGCAGC GCATTGCCTT ATCCAACAAT CATGAATACC 851 GGATGCAGCA GCTTGCCCTG CAAAGCAGCG GACAGGCGCT TCGGGCAGCA 901 CAGAACAGCC GCTATCCCAC CGTTTCTGCC CATGTCGGCT ATCAGAATAA 951 CCTCTACACT TCATCTGCGC AGAATAATGA CTACCACTAT CGGGGCAAAG 1001 GGATGAGCGT CGGCGTACAG TTGAATTTGC CGCTTTATAC CGGCGGAGAA 1051 TTGTCGGGCA AAATCCATGA AGCCGAAGCG CAATACGGGG CTGCCGAAGC 1101 ACAGCTGACC GCAACCGAGC GGCACATCAA ACTCGCCGTA CGCCAGGCTT 1151 ATACCGAAAG CGGTGCGGCG CGTTACCAAA TCATGGCGCA AGAACGGGTT 1201 TTGGAAAGCA GCCGTTTGAA ACTGAAATCG ACCGAAACCG GCCAACAATA 1251 CGGCATCCGC AACCGGCTGG AAGTAATACG GGCGCGGCAG GAAGTCGCCC 1301 AAGCAGAACA GAAACTGGCT CAAGCACGGT ATAAATTCAT GCTGGCTTAT 1351 TTGCGCTTGG TGAAAGAGAG CGGGTTAGGG TTGGAAACGG TATTTGCGGA 1401 ATAA This corresponds to the amino acid sequence <SEQ ID 2618; ORF 763. a>: a763.pep 1 MTLLNLMIMQ DYGISVCLTL TPYLQHELFS AMKSYFSKYI LPVSLFTLPL 51 SLSPSVSAFT LPEAWRAAQQ HSADFQASHY QRDAVRARQQ QAKAAFLPHV 101 SANASYQRQP PSISSTRETQ GWSVQVGQTL FDAAKFAQYR QSRFDTQAAE 151 QRFDAAREEL LLKVAESYFN VLLSRDTVAA HAAEKEAYAQ QVRQAQALFN 201 KGAATALDIH EAKAGYDNAL AQEIAVLAEK QTYENQLNDY TGLDSKQIEA 251 IDTANLLARY LPKLERYSLD EWQRIALSNN HEYRMQQLAL QSSGQALRAA 301 QNSRYPTVSA HVGYQNNLYT SSAQNNDYHY RGKGMSVGVQ LNLPLYTGGE 351 LSGKIHEAEA QYGAAEAQLT ATERHIKLAV RQAYTESGAA RYQIMAQERV 401 LESSRLKLKS TETGQQYGIR NRLEVIRARQ EVAQAEQKLA QARYKFMLAY 451 LRLVKESGLG LETVFAE* m763 / a763 99.8% identity in 467 aa overlap 10 20 30 40 50 60 m763.pep MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVSLFTLPLSLSPSVSAFT II I II I II II II II II II I I I II II II II I I I II I I I I II II I I I I I I I I I I II I I I I II a763 MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVSLFTLPLSLSPSVSAFT 10 20 30 40 50 60 70 80 90 100 110 120 m763.pep LPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSANASYQRQPPSISSTRETQ MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a763 LPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSANASYQRQPPSISSTRETQ 70 80 90 100 110 120 130 140 150 160 170 180 m763. pep GWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREELLLKVAESYFNVLLSRDTVAA I I II I I I I II II I I II I I II II II II I II II II II I I II II II II II I II II I II II I I I a763 GWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREELLLKVAESYFNVLLSRDTVAA 130 140 150 160 170 180 190 200 210 220 230 240 m763. pep HAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDNALAQEIAVLAEKQTYENQLNDY I II II I I I II II I II II II II I I II I I I I II II II I II II II II I I I II I I I II II II I I a763 HAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDNALAQEIAVLAEKQTYENQLNDY 190 200 210 220 230 240 250 260 270 280 290 300 m763.pep TDLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIALSNNHEYRMQQLALQSSGQALRAA I II II II I I II II II II I II I II II I I I II II II II I II I I I I I II II II I I II II II I a763 TGLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIALSNNHEYRMQQLALQSSGQALRAA 250 260 270 280 290 300 310 320 330 340 350 360 m763. pep QNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKGMSVGVQLNLPLYTGGELSGKIHEAEA I II II II II I II II II II I II I II II I I II II II I II II II II I II I I II II I II II II I a763 QNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKGMSVGVQLNLPLYTGGELSGKIHEAEA 310 320 330 340 350 360 370 380 390 400 410 420 m763. pep QYGAAEAQLTATERHIKLAVRQAYTESGAARYQIMAQERVLESSRLKLKSTETGQQYGIR I II II I I II I I II II II II II II II II II II II II I II I II II I I II II I II II I I II II a763 QYGAAEAQLTATERHIKLAVRQAYTESGAARYQIMAQERVLESSRLKLKSTETGQQYGIR 370 380 390 400 410 420 430 440 450 460 m763. pep NRLEVIRARQEVAQAEQKLAQARYKFMLAYLRLVKESGLGLETVFAEX I I I II II II II I II I I II II II II I I II II II II II II II I II I II I I a763 NRLEVIRARQEVAQAEQKLAQARYKFMLAYLRLVKESGLGLETVFAEX 430 440 450 460 g764.seq not found yet g764.pep not found yet The following partial DNA sequence was identified in N meningitidis <SEQ ID 2619>: m764.seq 1 ATGTTTTTCT CCGCCCTGAA ATCCTTTCTT TCTCGATACA TTACTGTATG 51 GCGCAATGTT TGGGCGGTGC GCGACCAGTT GAAACCGCCC AAACGCACGG 101 CGGAAGAACA GGCGTTTTTG CCCGCGCATT TGGAACTGAC CGATACGCCG 151 GTCTCTGCCG CTCCGAAATG GGCGGCGCGT TTTATTATGG CGTTTGCGCT 201 TTTGGCTTTG TTGTGGTCCT GGTTCGGCAA AATCGATATT GTGGCGGCGG 251 CTTCGGGCAA AACGGTGTCG GGCGGGCGCA GCAAAACCAT CCAGCCGCTG 301 GAAACGGCGG TGGTTAAGGC GGTACATGTG CGCGACGGGC AGCATGTGAA 351 ACAGGGAGAA ACGCTGGCGG AACTGGAGGC TGTGGGAACA GACAGCGATG 401 TGGTGCAGTC GGAGCAGGCT TTGCAGGCTG CCCAATTGTC CAAACTGCGT 451 TATGAAGCGG TATTGGCGGC ATTGGAAAGC CGTACCGTGC CGCATATCGA 501 TATGGCGCAA GCACGGTCTT TAGGTCTCTC CGATGCCGAT GTGCAATCGG 551 CGCAGGTGTT GGCGCAGCAC CAGTATCAGG CATGGGCGGC GCAGGATGCG 601 CAATTGCAGT CGGCTTTGCG CGGCCATCAG GCGGAATTGC AGTCGGCCAA 651 GGCGCAGGAG CAGAAGCTGG TTTCGGTGGG GGCGATCGAG CAGCAGAAAA 701 CAGCAGACTA CCGCCGTTTG CGGGCCGACA ATTTTATTTC GGAACATGCG 751 TTTTTGGAGC AGCAGAGCAA ATCGGTCAGC AATTGGAACG ATTTGGAAAG 801 TACGCGCGGT CAGATGAGGC AGATTCAGGC GGCCATTGCA CAGGCGGAGC 851 AGAATCGGGT GCTGAATACG CAGAACCTGA AACGCGATAC GCTGGATGCG 901 CTGCGCCAGG CAAACGAACA GATTGACCAA TACCGCGGCC AAACGGATAA 951 GGCAAAGCAG CGGCAGCAGC TGATGACAAT ACAGTCGCCT GCGGACGGCA 1001 CGGTGCAGGA ATTGGCTACC TATACGGTGG GCGGTGTGGT GCAGGCTGCC 1051 CAAAAAATGA TGGTGATTGC GCCCGATGAC GACAAAATGG ACGTGGAAGT 1101 TTTGGTATTG AACAAAGACA TCGGTTTTGT GGAACAGGGA CAGGATGCGG 1151 TGGTGAAGAT TGAGAGCTTT CCCTATACGC GCTACGGTTA TCTGACGGGC 1201 AAGGTGAAAA GTGTCAGCCA TGATGCGGTA AGCCACGAAC AGTTGGGCTT 1251 GGTTTATACG GCGGTGGTGT CGCTGGACAA ACATACCTTG AATATTGACG 1301 GCAAAGCAGT GAATCTGACG GCGGGCATGA ATGTCACGGC GGAGATTAAA 1351 ACGGGTAAAC GGCGGGTGCT GGATTATCTG TTAAGCCCGC TGCAAACCAA 1401 ATTGGACGAA AGCTTTAGGG AGCGATAG This corresponds to the amino acid sequence <SEQ ID 2620; ORF 764>: m764.pep 1 MFFSALKSFL SRYITVWRΝV WAVRDQLKPP KRTAEEQAFL PAHLELTDTP 51 VSAAPKWAAR FIMAFALLAL LWSWFGKIDI VAAASGKTVS GGRSKTIQPL 101 ETAVVKAVHV RDGQHVKQGE TLAELEAVGT DSDWQSEQA LQAAQLSKLR 151 YEAVLAALES RTVPHIDMAQ ARSLGLSDAD VQSAQVLAQH QYQAWAAQDA 201 QLQSALRGHQ AELQSAKAQE QKLVSVGAIE QQKTADYRRL RADΝFISEHA 251 FLEQQSKSVS ΝWΝDLESTRG QMRQIQAAIA QAEQΝRVLΝT QΝLKRDTLDA 301 LRQAΝEQIDQ YRGQTDKAKQ RQQLMTIQSP ADGTVQELAT YTVGGWQAA 351 QKMMVIAPDD DKMDVEVLVL ΝKDIGFVEQG QDAWKIESF PYTRYGYLTG 401 KVKSVSHDAV SHEQLGLVYT AVVSLDKHTL ΝIDGKAVΝLT AGMΝVTAEIK 451 TGKRRVLDYL LSPLQTKLDE SFRER* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2621>: a764.seq (partial) 1 ATGTTTTTCT CCGCCCTGAA ATCCTTTCTT TCCCGCTACA TTACCGTATG 51 GCGCAATGTT TGGGCGGTGC GCGACCAGTT GGAACCGCCC AAACGCACGG 101 CGGAAGAACA GGCGTTTTTG CCCGCGCATT TGGAACTGAC CGATACGCCG 151 GTCTCTGCCG CTCCGAAATG GGCGGCGCGT TTTATTATGG CGTTTGCGCT 201 TTTGGCTTTG TTGTGGTCCT GGTTCGGCAA AATCGATATT GTGGCGGCGG 251 CTTCGGGCAA AACGGTGTCG GGCGGGCGCA GCAAAACCAT CCAGCCGCTG 301 GAAACGGTGG TGGTTAAGGC GGTACATGTG CGCGACGGGC AGCATGTGAA 351 ACAGGGAGAA ACGCTGGCGG AACTGGAGGC TGTGGGAACA GACAGCGATG 401 TGGTGCAGTC GGAGCAGGCT TTGCAGGCTG CCCAATTGTC CAAACTGCGT 451 TATGAAGCGG TATTGGCGGC ATTGGAAAGC CGTACCGTGC CGCATATCGA 501 TATGGCGCAA GCACGGTCTT TAGGTCTCTC CGATGCCGAT GTGCAATCGG 551 CGCAGGTGTT GGCGCAGCAC CAGTATCAGG CATGGGCGGC GCAGGATGCG 601 CAATTGCAGT CGGCTTTGCG CGGCCATCAG GCGGAATTGC AGTCGGCCAA 651 GGCGCAGGAG CAGAAGCTGG TTTCGGTGGG GGCGATCGAG CAGCAGAAAA 701 CAGCAGACTA CCGCCGTTTG CGGGCCGACA ATTTTATTTC GGAACATGCG 751 TTTTTGGAGC AGCAGAGCAA ATCGGTCAGC AATTGGAACG ATTTGGAAAG 801 TACGCGCGGT CAGATGAGGC AGATTCAGGC GGCCATTGCA CAGGCGGAGC 851 AGAATCGGGT GCTGAATACG CAGAACCTGA AACGCGATAC GCTGGATGCG 901 CTGCGCCAGG CAAACGAACA GATTGACCAA TACCGCGGCC AAACGGATAA 951 GGCAAAGCAG CGGCAGCAGC TGATGACAAT ACAGTCGCCT GCGGACGGCA 1001 CGGTGCAGGA ATTGGCCACC TATACGGTGG GCGGTGTGGT GCAGGCTGCC 1051 CAAAAAATGA TGGTGGTTGC GCCCGATGAC GACAAAATGG ACGTGGAAGT 1101 TTTGGTATTG AACAAAGACA TCGGTTTTGT GGAACAGGGA CAGGATGCGG 1151 TGGTGAAGAT TGAGAGTTTT CCCTATACGC GCTACGGTTA TCTGACGGGC 1201 AAGGTGAAAA GTGTCAGCCA TGATGCGGTA AGCCACGAAC AGTTGGGCTT 1251 GGTTTATACG GCGGTGGTGT CGCTGGACAA ACATACCTTG AATATTGACG 1301 GCAAA This corresponds to the amino acid sequence <SEQ ID 2622; ORF 764.a>: a764. ep (partial) 1 MFFSALKSFL SRYITVWRNV WAVRDQLEPP KRTAEEQAFL PAHLELTDTP 51 VSAAPKWAAR FIMAFALLAL LWSWFGKIDI VAAASGKTVS GGRSKTIQPL 101 ETVVVKAVHV RDGQHVKQGE TLAELEAVGT DSDWQSEQA LQAAQLSKLR 151 YEAVLAALES RTVPHIDMAQ ARSLGLSDAD VQSAQVLAQH QYQAWAAQDA 201 QLQSALRGHQ AELQSAKAQE QKLVSVGAIE QQKTADYRRL RADNFISEHA 251 FLEQQSKSVS NWNDLESTRG QMRQIQAAIA QAEQNRVLNT QNLKRDTLDA 301 LRQANEQIDQ YRGQTDKAKQ RQQLMTIQSP ADGTVQELAT YTVGGWQAA 351 QKMMVVAPDD DKMDVEVLVL NKDIGFVEQG QDAWKIESF PYTRYGYLTG 401 KVKSVSHDAV SHEQLGLVYT AVVSLDKHTL NIDGK m764 / a764 99.3% identity in 435 aa overlap 10 20 30 40 50 60 m764. pep MFFSALKSFLSRYITVWRNVWAVRDQLKPPKRTAEEQAFLPAHLELTDTPVSAAPKWAAR I I I I I II I II II I I II I I II II I I I II : I I II I II I I I II I I I II I II I II I I I I II II I a764 MFFSALKSFLSRYITVWRNVWAVRDQLEPPKRTAEEQAFLPAHLELTDTPVSAAPKWAAR 10 20 30 40 50 60 70 80 90 100 110 120 m764. pep FIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLETAVVKAVHVRDGQHVKQGE I II II II II I I II II I II II II I I II II I I I I II II I I I II M I I I I I II II II I II I I I a764 FIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLETVWKAVHVRDGQHVKQGE 70 80 90 100 110 120 130 140 150 160 170 180 m764. pep TLAELEAVGTDSDWQSEQALQAAQLSKLRYEAVLAALESRTVPHIDMAQARSLGLSDAD MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a764 TLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALESRTVPHIDMAQARSLGLSDAD 130 140 150 160 170 180 190 200 210 220 230 240 m764. pep VQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKAQEQKLVSVGAIEQQKTADYRRL I II I II II II I II II I II II II I II II I I I I II II II II II I I I II I I II II I II I II II a764 VQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKAQEQKLVSVGAIEQQKTADYRRL 190 200 210 220 230 240 250 260 270 280 290 300 m764. pep RADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQAAIAQAEQNRVLNTQNLKRDTLDA MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a764 RADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQAAIAQAEQNRVLNTQNLKRDTLDA 250 260 270 280 290 300 310 320 330 340 350 360 m764. pep LRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGTVQELATYTVGGVVQAAQKMMVIAPDD MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a764 LRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGTVQELATYTVGGWQAAQKMMVVAPDD 310 320 330 340 350 360 370 380 390 400 410 420 m76 . pep DKMDVEVLVLNKDIGFVEQGQDAVVKIESFPYTRYGYLTGKVKSVSHDAVSHEQLGLVYT I II II I I II II I I I I II II II I II I II I II I II II II I I II I I I II I II I I I I II I I I II a764 DKMDVEVLVLNKDIGFVEQGQDAWKIESFPYTRYGYLTGKVKSVSHDAVSHEQLGLVYT 370 380 390 400 410 420 430 440 450 460 470 m76 . pep AWSLDKHTLNIDGKAVNLTAGMNVTAEIKTGKRRVLDYLLSPLQTKLDESFRERX II I I I II I II II II I a764 AWSLDKHTLNIDGK 430 g765.seq not yet found g765.pep not yet found The following partial DNA sequence was identified in N meningitidis <SEQ ID 2623>: m765. seq 1 ATGTTAAGAT GCCGTCCGAA ATCCGTTTTG GATTCAGACG GCATTTTTTT 51 GAAATTTAAT TTTTTAAGGA GTAAACCTAA ATATGAAATT TCCTTCCTTC 101 CTTCCTTTAA ACGGATACTC TGCCTGTCGG CAGTAATCTC GGTATTGGGG 151 GCTTGTGCGG TCGTTGCTGA TGTTTACGGT CATGATTCCG CCACAATGAA 201 CGCTGCGGCT GCCAAAGATT ATATGAAAAC GGTTGAGTTA AACAAGTCTG 251 CCGGCAATGT CGATACCACA TCCAGAACAG CCCGCAGGGT GCAGGCAGTA 301 TTTCGACGTA TGCTGCCTTA TGCCGATGCG GCAAATAATA CCAGCCATAA 351 GTTTGACTGG AAAATGACGG TTTTCAAAAA CGATGAGCTG AACGCGTGGG 401 CAATGCCCGG TGGAAAAATG GCGTTTTATA CGGGGATAGT CGACAAACTC 451 AAGCTGACCG ATGACGAAAT TGCCGCCATT ATGGGGCATG AAATGACGCA 501 CGCCCTGCAT GAACACGGTA AAAATAAGGT CGGGCAGCAA ATCTTGACCA 551 ATACGGCGGC GCAGATAGGC ACGCAGATTA TATTAGACAA AAAACCGGAT 601 ACTAATCCGG AATTGGTCGG ATTGGGTATG GATATTTTGG GGACGTACGG 651 TCTTACCTTG CCTTATAGCC GCAGCTTGGA AGAAGAAGCC GATGAGGGGG 701 GAATGATGTT GATGGCGCAG GCAGGCTATC ATCCGGCGGC CGCTGTCAGG 751 GTTTGGGAAA AAATGAATCA GGAAAACGAC CAAAACGGCT TTATTTATGC 801 TATTACCTCT ACTCATCCGA CAAACAATGC CCGTATAGAA AATCTAAAAC 851 GGTTGTTGCC GACCGTTATG CCGGTTTATG AGCAAAGTGT CAGAAATAAG 901 GGGCGCGTTA ATAAAAAACG TCGGCGTTAA This corresponds to the amino acid sequence <SEQ ID 2624; ORF 765>: m765.pep 1 MLRCRPKSVL DSDGIFLKFΝ FLRSKPKYEI ΞFLPΞFKRIL CLSAVISVLG 51 ACAWADVYG HDSATMΝAAA AKDYMKTVEL ΝKSAGΝVDTT SRTARRVQAV 101 FRRMLPYADA AΝΝTSHKFDW KMTVFKΝDEL ΝAWAMPGGKM AFYTGIVDKL 151 KLTDDEIAAI MGHEMTHALH EHGKΝKVGQQ ILTΝTAAQIG TQIILDKKPD 201 TΝPELVGLGM DILGTYGLTL PYΞRSLEEEA DEGGMMLMAQ AGYHPAAAVR 251 VWEKMΝQEΝD QΝGFIYAITS THPTΝΝARIE ΝLKRLLPTVM PVYEQΞVRΝK 301 GRVΝKKRRR* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2625>: a765.seq 1 ATGTTAAGAT GCCGTCCGAA ATCCGTTTTG GATTCAGACG GCATTTTTTT 51 GAAATTTAAT TTTTTAAGGA GTAAACCTAA ATATGAAATT TCCTTCCTTC 101 CTTCCTTTAA ACGGATACTC TGCCTGTCGG CAGTAATCTC GGTATTGGGG 151 GCTTGTACGG TCGTTGCTGA TGTTTACGGT CAGGATTCCG CCACAATGAA 201 TGCTGCGGCT GCCGAAGATT ATATGAAAAC GGTTGAGTTG AACAAGTCTG 251 CCGGCAATGT CGATACTACA TCCAAAACAG CCCGTAGGGT GCAGGCAGTA 301 TTTCGACGTA TGTTGCCTTA TGCCGATGCG GCAAATAATA CCGGCCATAA 351 GTTTGACTGG AAAATGACGG TTTTCAAAAA CGATGAGCTG AACGCGTGGG 401 CAATGCCCGG CGGGAAAATG GCGTTTTATA CGGGGATAGT CGATAAACTT 451 AAGCTGACCG ATGGCGAAAT TGCCGCCATT ATGGGGCATG AAATGACGCA 501 TGCCCTGCAT GAACACGGTA AAAATAAGGT CGGGCAGAAA ATCTTGACTA 551 ATATGGCGGC GCAGATAGGC ACGCAGATTA TATTAGACAA AAAACCGGAC 601 ACTAATCCGG AATTGGTCGG ATTGGGTATG GATATTTTGG GGATGTACGG 651 CATTACCTTG CCTTATAGCC GCAGCTTGGA AGAAGAAGCC GATGAGGGGG 701 GAATGATGTT GATGGCGCAG GCAGGCTATC ATCCGGCAGC CGCTGTCAGG 751 GTTTGGGAAA AAATGAATCA GGAAAACGAC CAAAACGGCT TTATTTATGC 801 TATTACCTCT ACTCATCCGA CAAACAATGC CCGTATAGAA AATCTAAAAC 851 GGTTGTTGCC GACCGTTATG CCGGTTTATG AGCACAGTGT TAGAAATAAG 901 GGGCGCGTTA ATAAAAACCG TCGGCGTTAA This corresponds to the amino acid sequence <SEQ ID 2626; ORF 765. a>: a765.pep 1 MLRCRPKSVL DSDGIFLKFΝ FLRSKPKYEI SFLPSFKRIL CLSAVISVLG 51 ACTWADVYG QDSATMNAAA AEDYMKTVEL NKSAGNVDTT ΞKTARRVQAV 101 FRRMLPYADA ANNTGHKFDW KMTVFKNDEL NAWAMPGGKM AFYTGIVDKL 151 KLTDGEIAAI MGHEMTHALH EHGKNKVGQK ILTNMAAQIG TQIILDKKPD 201 TNPELVGLGM DILGMYGITL PYSRSLEEEA DEGGMMLMAQ AGYHPAAAVR 251 VWEKMNQEND QNGFIYAITS THPTNNARIE NLKRLLPTVM PVYEHSVRNK 301 GRVNKNRRR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 765 shows 96.18% identity over a 309 aa overlap with a predicted ORF (ORF 765) from N. meningitidis: m765 / a765 96.1% identity in 309 aa overlap 10 20 30 40 50 60 m765.pep MLRCRPKSVLDSDGIFLKFΝFLRSKPKYEISFLPSFKRILCLSAVISVLGACAWADVYG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I a765 MLRCRPKSVLDSDGIFLKFΝFLRSKPKYEISFLPSFKRILCLSAVISVLGACTVVADVYG 10 20 30 40 50 60 70 80 90 100 110 120 m765. pep HDSATMΝAAAAKDYMKTVELΝKSAGΝVDTTSRTARRVQAVFRRMLPYADAAΝΝTSHKFDW M I I I I I I I I I : I I I I I I I I I I I I I I I I I I M I I I I I I I I I I I I I I I I I I I I I M I I I I I a765 QDSATMΝAAAAEDYMKTVELΝKSAGΝVDTTSKTARRVQAVFRRMLPYADAAΝΝTGHKFDW 70 80 90 100 110 120 130 140 150 160 170 180 m765. pep KMTVFKΝDELΝAWAMPGGKMAFYTGIVDKLKLTDDEIAAIMGHEMTHALHEHGKΝKVGQQ I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I 1 I I I I I I I I I I I I I I I M a765 KMTVFKΝDELΝAWAMPGGKMAFYTGIVDKLKLTDGEIAAIMGHEMTHALHEHGKΝKVGQK 130 140 150 160 170 180 190 200 210 220 230 240 m765.pep ILTΝTAAQIGTQIILDKKPDTΝPELVGLGMDILGTYGLTLPYSRSLEEEADEGGMMLMAQ llll I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M I I I I I I I I I I I I I I I I I I I I I I a765 ILTΝMAAQIGTQIILDKKPDTΝPELVGLGMDILGMYGITLPYSRΞLEEEADEGGMMLMAQ 190 200 210 220 230 240 250 260 270 280 290 300 m765. ep AGYHPAAAVRVWEKMΝQEΝDQΝGFIYAITSTHPTΝΝARIEΝLKRLLPTVMPVYEQSVRΝK I II I II I I I II I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I MM I I I I a765 AGYHPAAAVRVWEKMΝQEΝDQΝGFIYAITSTHPTΝΝARIEΝLKRLLPTVMPVYEHΞVRΝK 250 260 270 280 290 300 310 m765.pep GRVΝKKRRRX I I I I M I I I I a765 GRVΝKΝRRRX 310 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 2627>: g767.seq 1 ATGAAGTTTA AACATCTGTT GCCGCTGCTG CTGTCGGCAG TGTTGTCCGC 51 GCAGGCATAT GCCCTGACGG AAGGGGAAGA CTATCTTGTG TTGGATAAAC 101 CCATTCCTCA AGAACAGCCG GGAAAAATTG AGGTTTTGGA ATTTTTCGGC 151 TATTTTTGCG TACATTGCCA TCATTTCGAT CCTTTGTTAT TGAAACTGGG 201 CAAGGCATTG CCGTCTGATA CTTATCTGCG GACGGAGCAC GTGGTCTGGC 251 GGCCTGAAAT GCTCGGTCTG GCAAGAATGG CTGCTGCGGT CAAGCTGTCG 301 GGTTTGAAAT ATCAGGCAAA CTCTGCTGTG TTTAAAGCAG TTTACGAACA 351 AAAAATCCGT TTGGAAAACA GGGCTGTTGC CGGGAAATGG GCTTTATCTC 401 AAAAAGGTTT TGACGGCAAA AAACTGATGC GCGCCTATGA TTCCCCCGAA 451 GCTGCCGCCG TCGCATTAAA AATGCAGAAA CTGACGGAAC AATACGGTAT 501 TGACAGCACG CCGACCGTTA TTGTCGGCGG AAAATACCGC GTTATCTTCA 551 ATAATGGCTT TGATGGCGGC GTTCATACGA TTAAAGAATT GGTTGCCAAA 601 GTCAGGGAAG AACGCAAGCG TCAGACCCCT GCTGTACAGA AATAG This corresponds to the amino acid sequence <SEQ ID 2628; ORF 767.ng>: g767.pep 1 MKFKHLLPLL LSAVLSAQAY ALTEGEDYLV LDKPIPQEQP GKIEVLEFFG 51 YFCVHCHHFD PLLLKLGKAL PSDTYLRTEH WWRPEMLGL ARMAAAVKLS 101 GLKYQANΞAV FKAVYEQKIR LENRAVAGKW ALSQKGFDGK KLMRAYDSPE 151 AAAVALKMQK LTEQYGIDST PTVIVGGKYR VIFNNGFDGG VHTIKELVAK 201 VREERKRQTP AVQK* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2629>: m767.seq 1 ATGAAGCTCA AACATCTGTT GCCGCTGCTG CTGTCGGCAG TGTTGTCCGC 51 GCAGGCATAT GCCCTGACGG AAGGGGAAGA CTATCTTGTG TTGGATAAAC 101 CCATTCCTCA AGAACAGTCG GGTAAAATTG AGGTTTTGGA ATTTTTCGGC 151 TATTTCTGCG TACATTGCCA TCATTTCGAT CCTTTGTTAT TGAAACTGGG 201 CAAGGCATTG CCGTCTGATG CCTATTTGAG GACGGAGCAC GTGGTCTGGC 251 AGCCTGAAAT GCTCGGTTTG GCTAGGATGG CGGCTGCCGT CAATTTGTCG 301 GGTTTGAAAT ATCAGGCAAA CCCTGCTGTG TTTAAAGCAG TTTACGAACA 351 AAAAATCCGC TTGGAAAACA GGTCGGTTGC CGGAAAATGG GCTTTGTCTC 401 AAAAAGGCTT TGACGGCAAA AAACTGATGC GCGCCTATGA TTCCCCCGAA 451 GCTGCCGCCG CCGCATTAAA AATGCAGAAA CTGACGGAAC AATACCGCAT 501 CGACAGCACG CCGACCGTTA TTGTCGGCGG AAAATACCGC GTTATCTTCA 551 ATAACGGCTT TGACGGCGGC GTTCATACGA TTAAAGAATT GGTTGCCAAA 601 GTCAGGGAAG AACGCAAGCG TCAGACCCCT GCTGTACAGA AATAG This corresponds to the amino acid sequence <SEQ ID 2630; ORF 767>: m767. ep 1 MKLKHLLPLL LSAVLSAQAY ALTEGEDYLV LDKPIPQEQS GKIEVLEFFG 51 YFCVHCHHFD PLLLKLGKAL PSDAYLRTEH WWQPEMLGL ARMAAAVΝLS 101 GLKYQAΝPAV FKAVYEQKIR LEΝRSVAGKW ALSQKGFDGK KLMRAYDSPE 151 AAAAALKMQK LTEQYRIDST PTVIVGGKYR VIFΝΝGFDGG VHTIKELVAK 201 VREERKRQTP AVQK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 767 shows 95.8% identity over a 214 aa overlap with a predicted ORF (ORF 767) from N gonorrhoeae m767/g767 95.8% identity m 214 aa overlap 10 20 30 40 50 60 g767 . pep MKFKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQEQPGKIEVLEFFGYFCVHCHHFD I M M I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m767 MKLKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQEQSGKIEVLEFFGYFCVHCHHFD 10 20 30 40 50 60 70 80 90 100 110 120 g76 . pep PLLLKLGKALPSDTYLRTEHWWRPEMLGLARMAAAVKLSGLKYQAΝSAVFKAVYEQKIR I I I I I I I I I I I I Ml I I I I I I I M I I I I I I I I I I I I Ml I I I I I I I I I I I I I I I I I I I I m767 PLLLKLGKALPSDAYLRTEHWWQPEMLGLARMAAAVΝLSGLKYQAΝPAVFKAVYEQKIR 70 80 90 100 110 120 130 140 150 160 170 180 g767.pep LEΝRAVAGKWALSQKGFDGKKLMRAYDSPEAAAVALKMQKLTEQYGIDSTPTVIVGGKYR I I I M I I I I I I I I I I I I I I I I I I I I I I I I I I I I M I I I I I I I I I I llllllllllllll m767 LEΝRSVAGKWALSQKGFDGKKLMRAYDSPEAAAAALKMQKLTEQYRIDSTPTVIVGGKYR 130 140 150 160 170 180 190 200 210 g767. pep VIFΝΝGFDGGVHTIKELVAKVREERKRQTPAVQKX II I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I m767 VIFΝΝGFDGGVHTIKELVAKVREERKRQTPAVQKX 190 200 210 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2631>: a76 . seq 1 ATGAAGCTCA AACATCTGTT GCCGCTGCTG CTGTCGGCAG TGTTGTCCGC 51 GCAGGCATAT GCCCTGACGG AAGGGGAAGA CTATCTTGTG TTGGATAAAC 101 CCATTCCTCA AAAACAGTCG GGCAAAATTG AGGTTTTGGA ATTTTTCGGC 151 TATTTCTGCG TACATTGCCA TCATTTCGAT CCTTTGTTAT TGAAATTGGG 201 CAAGGCATTG CCGTCTGATG CCTATTTAAG GACGGAGCAC GTGGTCTGGC 251 AGCCTGAAAT GCTCGGTCTG GCAAGAATGG CTGCTGCGGT CAAGCTGTCA 301 GGTTTGAAAT ATCAGGCAAA CCCTGCCGTG TTTAAAGCAG TTTACGAACA 351 AAAAATCCGC TTGGAAAACA GGTCGGTTGC CGAAAAATGG GCTTTGTCTC 401 AAAAAGGCTT TGACGGCAAA AAACTGATGC GCGCCTACGA CTCTCCTGCG 451 GCAGCGGCTG CTGCATCAAA AATGCAGCAA TTGACGGAAC AGTACCGCAT 501 CGACAGTACG CCGACCGTTG TCGTCGGCGG AAAATACCGC GTTATCTTCA 551 ATAATGGCTT TGACGGCGGT GTTCATACGA TTAAAGAATT GGTTGCCAAA 601 GTCAGGGAAG AACGCAAGCG TCAGACCCCT GCTGTACAGA AATAG This corresponds to the amino acid sequence <SEQ ID 2632; ORF 767. a>: a767.pep 1 MKLKHLLPLL LSAVLSAQAY ALTEGEDYLV LDKPIPQKQS GKIEVLEFFG 51 YFCVHCHHFD PLLLKLGKAL PSDAYLRTEH WWQPEMLGL ARMAAAVKLS 101 GLKYQANPAV FKAVYEQKIR LENRSVAEKW ALSQKGFDGK KLMRAYDSPA 151 AAAAASKMQQ LTEQYRIDST PTVWGGKYR VIFNNGFDGG VHTIKELVAK 201 VREERKRQTP AVQK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 767 shows 96.7% identity over a 214 aa overlap with a predicted ORF (ORF 767) from N meningitidis: m767/a767 96.7% identity in 214 aa overlap 10 20 30 40 50 60 a767.pep MKLKHLLPLLLΞAVLSAQAYALTEGEDYLVLDKPIPQKQSGKIEVLEFFGYFCVHCHHFD II I II I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I M I I I I I I I I I M I I I I I I I I I I m767 MKLKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQEQSGKIEVLEFFGYFCVHCHHFD 10 20 30 40 50 60 70 80 90 100 110 120 a767.pep PLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVKLSGLKYQAΝPAVFKAVYEQKIR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I m767 PLLLKLGKALPSDAYLRTEHWWQPEMLGLARMAAAVΝLSGLKYQAΝPAVFKAVYEQKIR 70 80 90 100 110 120 130 140 150 160 170 180 a767 . pep LEΝRSVAEKWALSQKGFDGKKLMRAYDSPAAAAAAΞKMQQLTEQYRIDΞTPTVVVGGKYR l l l l l l l I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M I I I I I I I I I I I I : I I I I I I m767 LEΝRSVAGKWALSQKGFDGKKLMRAYDSPEAAAAALKMQKLTEQYRIDΞTPTVIVGGKYR 130 140 150 160 170 180 190 200 210 a767. pep VIFΝΝGFDGGVHTIKELVAKVREERKRQTPAVQKX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m767 VIFΝΝGFDGGVHTIKELVAKVREERKRQTPAVQKX 190 200 210 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 2633>: g768.seq 1 ATGAATATCA AACAATTGAT TACCGCCGCA CTCATTGCCT CAGCCGCCTT 51 TGCCACGCAG GCAGCCCCGC AAAAACCCGT ATCCGCCGCC CAAACCGCGC 101 AACATTCAGC CGTTTGGATC GATGTCCGTT CCGAACAGGA ATTTAGCGAA 151 GGTCATTTGC ACAACGCGGT CAACATCCCC GTCGACCAAA TCGTCCGCCG 201 CATATACGAA GCCGCGCCCG ACAAAGACAC GCCGGTCAAC CTCTACTGCC 251 GCAGCGGACG GCGTGCCGAA GCCGCCCTTC AAGAGCTGAA AAAAGCAGGT 301 TATACAAATG TTGCCAATCA CGGCGGTTAT GAAGACCTGC TCAAAAAAGG 351 GATGAAATGA This corresponds to the amino acid sequence <SEQ ID 2634; ORF 768. ng>: g768.pep 1 MΝIKQLITAA LIASAAFATQ AAPQKPVSAA QTAQHSAVWI DVRSEQEFΞE 51 GHLHΝAVΝIP VDQIVRRIYE AAPDKDTPVΝ LYCRSGRRAE AALQELKKAG 101 YTΝVAΝHGGY EDLLKKGMK* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2635>: 768.seq 1 ATGAATATCA AACACCTGAT TACCGCCGCA CTCATTGCCT CAGCCGCCTT 51 TGCCGCGCAG GCAGCCCCGC AAAAACCCGT ATCCGCCGCC CAAACCGCGC 101 AACATCCGGC CGTTTGGATC GATGTCCGTT CCGAACAGGA ATTTAGCGAA 151 GGGCATTTGC ACAACGCGGT CAACATCCCC GTCGACCAAA TCGTCCGCCG 201 CATACACGAA GCCGCGCCCG ACAAAGACAC GCCGGTCAAC CTCTACTGCC 251 GCAGCGGACG GCGTGCCGAA GCCGCCCTTC AAGAGCTGAA AAAAGCAGGT 301 TATACAAATG TTGCCAATCA CGGCGGTTAT GAAGACCTGC TCAAAAAAGG 351 GATGAAATGA This corresponds to the amino acid sequence <SEQ ID 2636; ORF 768>: 768.pep 1 MNIKHLITAA LIASAAFAAQ AAPQKPVSAA QTAQHPAVWI DVRSEQEFSE 51 GHLHNAVNIP VDQIVRRIHE AAPDKDTPVN LYCRSGRRAE AALQELKKAG 101 YTNVANHGGY EDLLKKGMK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 768 shows 96.6% identity over a 119 aa overlap with a predicted ORF (ORF 768) from N gonorrhoeae m768/g768 96.6% identity in 119 aa overlap 10 20 30 40 50 60 g768.pep MΝIKQLITAALIASAAFATQAAPQKPVSAAQTAQHSAVWIDVRSEQEFSEGHLHΝAVΝIP I II IM I I I I I I I I I I I Ml I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I m768 MΝIKHLITAALIASAAFAAQAAPQKPVΞAAQTAQHPAVWIDVRSEQEFSEGHLHΝAVΝIP 10 20 30 40 50 60 70 80 90 100 110 120 g768.pep VDQIVRRIYEAAPDKDTPVΝLYCRSGRRAEAALQELKKAGYTΝVAΝHGGYEDLLKKGMKX I II I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II m768 VDQIVRRIHEAAPDKDTPVΝLYCRSGRRAEAALQELKKAGYTΝVAΝHGGYEDLLKKGMKX 70 80 90 100 110 120 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2637>: a768.seq 1 ATGAATATCA AACACCTGAT TACCGCCGCA CTCATTGCCT CAGCCGCCTT 51 TGCCGCGCAG GCAGCCCCGC AAAAACCCGT ATCCGCCGCC CAAACCGCGC 101 AACATTCAGC CGTTTGGATC GATGTCCGCA GCGAACAGGA ATTTAGCGAA 151 GGTCATTTGC ACAACGCGGT CAACATCCCC GTCGACCAAA TCGTCCGCCG 201 CATACACGAA GCCGCGCCCG ACAAAGACAC GCCGGTCAAC CTCTACTGCC 251 GCAGCGGACG GCGTGCCGAA GCCGCCCTTC AAGAACTGAA AAAAGCAGGC 301 TATACGAATG TTGCCAATCA CGGCGGTTAT GAAGACCTGC TCAAAAAAGG 351 GATGAAATGA This corresponds to the amino acid sequence <SEQ ID 2638; ORF 768. a>: a768.pep 1 MNIKHLITAA LIASAAFAAQ AAPQKPVSAA QTAQHSAVWI DVRSEQEFSE 51 GHLHNAVNIP VDQIVRRIHE AAPDKDTPVN LYCRSGRRAE AALQELKKAG 101 YTNVANHGGY EDLLKKGMK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 768 shows 99.2% identity over a 119 aa overlap with a predicted ORF (ORF 768) from N meningitidis: 768/a768 99.2% identity in 119 aa overlap 10 20 30 40 50 60 a768.pep MΝIKHLITAALIASAAFAAQAAPQKPVSAAQTAQHSAVWIDVRSEQEFSEGHLHΝAVΝIP I I I I I I I I I I I II II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I m768 MΝIKHLITAALIASAAFAAQAAPQKPVSAAQTAQHPAVWIDVRSEQEFSEGHLHΝAVΝIP 10 20 30 40 50 60 70 80 90 100 110 120 a768.pep VDQIVRRIHEAAPDKDTPVΝLYCRSGRRAEAALQELKKAGYTΝVAΝHGGYEDLLKKGMKX I I I I I II I II I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I m768 VD Q IVRRIHEAAPDKDTPVΝLYCRSGRRAEAALQELKKAGYTΝVAΝHGGYEDLLKKGMKX 70 80 90 100 110 120 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2639>: g769.seq 1 TTGATAATGG TTATTTTTTA TTTTTATTTT TGTGGGAAGA CATTTATGCC 51 TGCACGAAAC AGATGGATGC TGCTGCCTTT ATTGGCAAGC GCGGCATACG 101 CCGAAgaAAC ACCgtgCGAA CCGGATTTGA GAAGCCGTCC CGAGTTCAGG 151 CTTCATGAAG CGGAGGTCAA ACCGATCGAC AGGGAGAAGG TACCGGGGCA 201 GGTGCGGGAA AAAGGAAAAG TTTTGCAGGT TGACGgcGAA ACCCTGCTGA 251 AAAATCCCGA ATTGTTGTCG CGTGCCATGT ATTCCGCAGT GGTCTCAAAC 301 AATATTGCCG GTATCCGCGT GATTTTGCCG ATTTACCTAC AACAGGCGCG 351 GCAGGATAAG ATGTTGGCAC TTTATGCACA AGGGATTTTG GCGCAGGCAG 401 AGGGCAGGGT GAAGGAGGCG GTTTCCCATT ACCGGGAATT GATTGCCGCC 451 CAACCCGACG CGCCCGCCGT CCGTATGCGT TTGGCGGCGG CATTGTTTGA 501 AGACAGGCAG AACGAGGCGG CGGCAGACCA GTTCGACCGC CTGAAAACAG 551 AAGATCTGCC GCCGCAGCTT ATGGAGCAGG TCGAGCTGTA CCGCAAGGCA 601 TTGCGCGAAC GCGATGCGTG GAAGGTAAAC GGCGGTTTCA GCGTTACCCG 651 CGAACACAAT ATCAACCAAG CCCCGAAACA GCAGCAGTAC GGCAATTGGA 701 CTTTCCCGAA ACAGGTGGAC GGCACGGCAG TCAATTACCG GTTCGGCGCG 751 GAGAAAAAAT GGTCGCTGAA AAACGGCTGG TACACGACGG CGGGCGGCGA 801 CGTGTCCGGC AGGGTTTATC CGGGGAATAA GAAATTCAAC GATATGACGG 851 CAGGTGTTTC CGGCGGCATC GGTTTTGCCG ACCGGCGTAA AGATGTCGGG 901 CTGGCAGTGT TCCACGAACG CCGCACCTAC GGCAACGACG CTTATTCTTA 951 CGCCAACGGC GCACGCCTTT ATTTCAACCG TTGGCAAACC CCGAGATGGC 1001 AAACGCTGTC TTCGGCGGAG TGGGGGCGTT TGAAGAATAC GCGCCGGGCG 1051 CGTTCCGACA ATACCCATTT GCAAATTTCC AATTCGCTGG TGTTTTACCG 1101 GAATGCGCGC CAATATTGGA CGGGCGGTTT GGATTTTTAC CGCGAGCGCA 1151 ACCCCGCCGA CCGTGGCGAC AATTTCAACC GTTACGGCCT GCGCTTTGCC 1201 TGGGGGCAGG AATGGGGCGG CAGCGGCCTG TCTTCGCTGT TCCGCCTCGG 1251 CGTGGCGAAA CGGCATTATG AAAAACCCGG CTTCTTCAGC AGTTTTAAAG 1301 GGGAAAGGCG CAGGGATAAA GAATCGGACA CATCCTTGAG CCTTTGGCAC 1351 CGGGCATTGC ATTTCAAAGG CATCACGCCG CGCCTGACGC TGTCGCACCG 1401 CGAAACGTGG AGCAACGATG TGTTTAACGA ATACGAGAAA AACAGGGCGT 1451 TTGTCGAGTT TAACAAAACG TTCTGA This corresponds to the amino acid sequence <SEQ ID 2640; ORF 769.ng>: g769.pep 1 LIMVIFYFYF CGKTFMPARΝ RWMLLPLLAΞ AAYAEETPCE PDLRSRPEFR 51 LHEAEVKPID REKVPGQVRE KGKVLQVDGE TLLKΝPELLS RAMYSAWΞΝ 101 ΝIAGIRVILP IYLQQARQDK MLALYAQGIL AQAEGRVKEA VSHYRELIAA 151 QPDAPAVRMR LAAALFEDRQ ΝEAAADQFDR LKTEDLPPQL MEQVELYRKA 201 LRERDAWKVΝ GGFΞVTREHΝ IΝQAPKQQQY GΝWTFPKQVD GTAVΝYRFGA 251 EKKWSLKΝGW YTTAGGDVSG RVYPGΝKKFΝ DMTAGVSGGI GFADRRKDVG 301 LAVFHERRTY GΝDAYSYAΝG ARLYFΝRWQT PRWQTLSSAE WGRLKΝTRRA 351 RSDΝTHLQIS ΝSLVFYRΝAR QYWTGGLDFY RERΝPADRGD ΝFΝRYGLRFA 401 WGQEWGGSGL SSLFRLGVAK RHYEKPGFFS SFKGERRRDK ESDTSLSLWH 451 RALHFKGITP RLTLΞHRETW SΝDVFΝEYEK ΝRAFVEFΝKT F* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2641>: m769.seq 1 TTGATAATGG TTATTTTTTA TTTTTGTGGG AAGACATTTA TGCCTGCACG 51 AAACAGATGG ATGCTGCTGC TGCCTTTATT GGCAAGCGCG GCATATGCCG 101 AAGAAACACC GCGCGAACCG GATTTGAGAA GCCGTCCCGA GTTCAGGCTT 151 CATGAAGCGG AGGTCAAACC GATCGACAGG GAGAAGGTGC CGGGGCAGGT 201 GCGGGAAAAA GGAAAAGTTT TGCAGATTGA CGGCGAAACC CTGCTGAAAA 251 ATCCCGAATT GTTGTCCCGC GCGATGTATT CCGCAGTGGT CTCAAACAAT 301 ATTGCCGGTA TCCGCGTTAT TTTGCCGATT TACCTACAAC AGGCGCAGCA 351 GGATAAGATG TTGGCACTTT ATGCACAAGG GATTTTGGCG CAGGCAGACG 401 GTAGGGTGAA GGAGGCGATT TCCCATTACC GGGAATTGAT TGCCGCCCAA 451 CCCGACGCGC CCGCCGTCCG TATGCGTTTG GCGGCAGCAT TGTTTGAAAA 501 CAGGCAGAAC GAGGCGGCGG CAGACCAGTT CGACCGCCTG AAGGCGGAAA 551 ACCTGCCGCC GCAGCTGATG GAGCAGGTCG AGCTGTACCG CAAGGCATTG 601 CGCGAACGCG ATGCGTGGAA GGTAAATGGC GGCTTCAGCG TCACCCGCGA 651 ACACAATATC AACCAAGCCC CGAAACGGCA GCAGTACGGC AAATGGACTT 701 TCCCGAAACA GGTGGACGGC ACGGCGGTCA ATTACCGGCT CGGCGCGGAG 751 AAAAAATGGT CGCTGAAAAA CGGCTGGTAC ACGACGGCGG GCGGCGACGT 801 GTCCGGCAGG GTTTATCCGG GGAATAAGAA ATTCAACGAT ATGACGGCAG 851 GCGTTTCCGG CGGCATCGGT TTTGCCGACC GGCGCAAAGA TGCCGGGCTG 901 GCAGTGTTCC ACGAACGCCG CACCTACGGC AACGACGCTT ATTCTTACAC 951 CAACGGCGCA CGCCTTTATT TCAACCGTTG GCAAACCCCG AAATGGCAAA 1001 CGTTGTCTTC GGCGGAGTGG GGGCGTTTGA AGAATACGCG CCGGGCGCGT 1051 TCCGACAATA CCCATTTGCA AATTTCCAAT TCGCTGGTGT TTTACCGGAA 1101 TGCGCGCCAA TATTGGATGG GCGGTTTGGA TTTTTACCGC GAGCGCAACC 1151 CCGCCGACCG GGGCGACAAT TTCAACCGTT ACGGCCTGCG CTTTGCCTGG 1201 GGGCAGGAAT GGGGCGGCAG CGGCCTGTCT TCGCTGTTGC GCCTCGGCGC 1251 GGCGAAACGG CATTATGAAA AACCCGGCTT TTTCAGCGGT TTTAAAGGGG 1301 AAAGGCGCAG GGATAAAGAA TTGAACACAT CCTTGAGCCT TTGGCACCGG 1351 GCATTGCATT TCAAAGGCAT CACGCCGCGC CTGACGTTGT CGCACCGCGA 1401 AACGCGGAGT AACGATGTGT TCAACGAATA CGAGAAAAAT CGGGCGTTTG 1451 TCGAGTTTAA TAAAACGTTC TGA This corresponds to the amino acid sequence <SEQ ID 2642; ORF 769>: m769.pep 1 LIMVIFYFCG KTFMPARNRW MLLLPLLASA AYAEETPREP DLRSRPEFRL 51 HEAEVKPIDR EKVPGQVREK GKVLQIDGET LLKNPELLSR AMYSAWSNN 101 IAGIRVILPI YLQQAQQDKM LALYAQGILA QADGRVKEAI SHYRELIAAQ 151 PDAPAVRMRL AAALFENRQN EAAADQFDRL KAENLPPQLM EQVELYRKAL 201 RERDAWKVNG GFSVTREHNI NQAPKRQQYG KWTFPKQVDG TAVNYRLGAE 251 KKWΞLKNGWY TTAGGDVSGR VYPGNKKFND MTAGVSGGIG FADRRKDAGL 301 AVFHERRTYG NDAYΞYTNGA RLYFNRWQTP KWQTLSSAEW GRLKNTRRAR 351 SDNTHLQISN SLVFYRNARQ YWMGGLDFYR ERNPADRGDN FNRYGLRFAW 401 GQEWGGSGLS SLLRLGAAKR HYEKPGFFSG FKGERRRDKE LNTSLSLWHR 451 ALHFKGITPR LTLSHRETRS NDVFNEYEKN RAFVEFNKTF * Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 169 shows 95.1% identity over a 492 aa overlap with a predicted ORF (ORF 769) from N gonorrhoeae m769/g769 95.1% identity in 492 aa overlap 10 20 30 40 50 59 g769.pep LIMVIFYFYFCGKTFMPARΝRWMLL-PLLASAAYAEETPCEPDLRSRPEFRLHEAEVKPI lllllll I I I I I I I I I I I I I I I I llllll l I I I I I I I I I I I I I I I I I I I I m769 LIMVIFY—FCGKTFMPARΝRWMLLLPLLASAAYAEETPREPDLRSRPEFRLHEAEVKPI 10 20 30 40 50 60 70 80 90 100 110 119 g769. pep DREKVPGQVREKGKVLQVDGETLLKΝPELLSRAMYSAWSΝΝIAGIRVILPIYLQQARQD I I I I I I I I I I I I I I I I M I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I m769 DREKVPGQVREKGKVLQIDGETLLKΝPELLΞRAMYSAVVSΝΝIAGIRVILPIYLQQAQQD 60 70 80 90 100 110 120 130 140 150 160 170 179 g769. pep KMLALYAQGILAQAEGRVKEAVSHYRELIAAQPDAPAVRMRLAAALFEDRQΝEAAADQFD I I I I I I I I II I I I M I I I I I M I I I I I I I I I I I I I I I I I I I I I I I I I Ml I I I I I I I I I I m769 KMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAAALFEΝRQΝEAAADQFD 120 130 140 150 160 170 180 190 200 210 220 230 239 g769.pep RLKTEDLPPQLMEQVELYRKALRERDAWKVΝGGFSVTREHΝIΝQAPKQQQYGΝWTFPKQV I I I : M I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I M I I I I : I I I I I I I m769 RLKAEΝLPPQLMEQVELYRKALRERDAWKVΝGGFSVTREHΝIΝQAPKRQQYGKWTFPKQV 180 190 200 210 220 230 240 250 260 270 280 290 299 g769.pep DGTAVΝYRFGAEKKWSLKΝGWYTTAGGDVSGRVYPGΝKKFΝDMTAGVSGGIGFADRRKDV I I I I II I M I I I I I I I I I I I I I I I I I II I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I : m769 DGTAVΝYRLGAEKKWSLKΝGWYTTAGGDVSGRVYPGΝKKFΝDMTAGVSGGIGFADRRKDA 240 250 260 270 280 290 300 310 320 330 340 350 359 g769.pep GLAVFHERRTYGΝDAYSYAΝGARLYFΝRWQTPRWQTLSSAEWGRLKΝTRRARSDΝTHLQI I I I I I I I I I I I I I I I I I I M I I I I I II I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I m769 GLAVFHERRTYGΝDAYSYTΝGARLYFΝRWQTPKWQTLSΞAEWGRLKΝTRRARSDΝTHLQI 300 310 320 330 340 350 360 370 380 390 400 410 419 g769.pep SΝSLVFYRΝARQYWTGGLDFYRERΝPADRGDΝFΝRYGLRFAWGQEWGGSGLSSLFRLGVA llllllllllllll I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I M m769 SΝSLVFYRΝARQYWMGGLDFYRERΝPADRGDΝFΝRYGLRFAWGQEWGGSGLSSLLRLGAA 360 370 380 390 400 410 420 430 440 450 460 470 479 g769.pep KRHYEKPGFFSSFKGERRRDKESDTSLSLWHRALHFKGITPRLTLΞHRETWSΝDVFΝEYE I I I I I I I I II I : I I I I I I I I I I : I II I I I I I I I I I I I I I I I I I I I I I I I ll l m769 KRHYEKPGFFSGFKGERRRDKELNTSLSLWHRALHFKGITPRLTLSHRETRSNDVFNEYE 420 430 440 450 460 470 480 490 g769.pep KNRAFVEFNKTFX I II I I II I II I I I m769 KNRAFVEFNKTFX 490 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2643>: a769.seq 1 TTGATAATGG TTATTTTTTA TTTTTGTGGG AAGACATTTA TGCCTGCACG 51 AAACAGATGG ATGCTGCTGC TGCCTTTATT GGCAAGCGCG GCATATGCCG 101 AAGAAACACC GCGCGAACCG GATTTGAGAA GCCGTCCCGA GTTCAGGCTT 151 CATGAAGCGG AGGTCAAACC AATCGACAGG GAGAAGGTAC CGGGGCAGGT 201 GCGGGAAAAA GGAAAAGTTT TGCAGATTGA CGGCGAAACC CTGCTGAAAA 251 ATCCCGAATT GCTGTCCCGC GCGATGTATT CCGCAGTGGT CTCAAACAAT 301 ATTGCCGGTA TCCGCGTTAT TTTGCCGATT TACCTACAAC AGGCGCAGCA 351 GGATAAGATG TTGGCACTTT ATGCACAAGG GATTTTGGCG CAGGCAGACG 401 GTAGGGTGAA GGAGGCGATT TCCCATTACC GGGAATTGAT TGTCGCCCAA 451 CCCGACGCGC CCGCCGTCCG TATGCGTTTG GCGGCGGCAT TGTTTGAAAA 501 CAGGCAGAAC GAGGCGGCGG CAGACCAGTT CGACCGCCTG AAGGCGGAAA 551 ACCTGCCGCC GCAGCTGATG GAGCAGGTCG AGCTGTACCG CAAGGCATTG 601 CGCGAACGCG ATGCGTGGAA GGTAAATGGC GGCTTCAGCG TTACCCGCGA 651 ACACAATATC AACCAAGCCC CGAAACGGCA GCAGTACGGC AAATGGACTT 701 TCCCGAAACA GGTGGACGGC ACGGCGGTCA ATTACCGGCT CGGCGCGGAG 751 AAAAAATGGT CGCTGAAAAA CGGCTGGTAC ACGACGGCGG GCGGCGACGT 801 GTCCGGCAGG GTTTATCCGG GGAATAAGAA ATTCAACGAT ATGACGGCAG 851 GCGTTTCCGG CGGCATCGGT TTTGCCGACC GGCGCAAAGA TGCCGGGCTG 901 GCAGTGTTCC ACGAACGCCG CACCTACGGC AACGACGCTT ATTCTTACAC 951 CAACGGCGCA CGCCTTTATT TCAACCGTTG GCAAACCCCG AAATGGCAAA 1001 CGTTGTCTTC GGCGGAGTGG GGGCGTTTGA AGAATACGCG CCGGGCGCGT 1051 TCCGACAATA CCCATTTGCA AATTTCCAAT TCGCTGGTGT TTTACCGGAA 1101 TGCGCGCCAA TATTGGATGG GCGGTTTGGA TTTTTACCGC GAGCGCAACC 1151 CCGCCGACCG GGGCGACAAT TTCAACCGTT ACGGCCTGCG CTTTGCCTGG 1201 GGGCAGGAAT GGGGCGGCAG CGGCCTGTCT TCGCTGTTGC GCCTCGGCGC 1251 GGCGAAACGG CATTATGAAA AACCCGGCTT TTTCAGCGGT TTTAAAGGGG 1301 AAAGGCGCAG GGATAAAGAA TTGAACACAT CCTTGAGCCT TTGGCACCGG 1351 GCATTGCATT TCAAAGGCAT CACGCCGCGC CTGACGTTGT CGCACCGCGA 1401 AACGCGGAGT AACGATGTGT TCAACGAATA CGAGAAAAAT CGGGCGTTTG 1451 TCGAGTTTAA TAAAACGTTC TGA This corresponds to the amino acid sequence <SEQ ID 2644; ORF 769. a>: a769.pep 1 LIMVIFYFCG KTFMPARΝRW MLLLPLLASA AYAEETPREP DLRSRPEFRL 51 HEAEVKPIDR EKVPGQVREK GKVLQIDGET LLKΝPELLSR AMYSAWSΝΝ 101 IAGIRVILPI YLQQAQQDKM LALYAQGILA QADGRVKEAI SHYRELIVAQ 151 PDAPAVRMRL AAALFEΝRQΝ EAAADQFDRL KAEΝLPPQLM EQVELYRKAL 201 RERDAWKVΝG GFSVTREHΝI ΝQAPKRQQYG KWTFPKQVDG TAVΝYRLGAE 251 KKWSLKΝGWY TTAGGDVSGR VYPGΝKKFΝD MTAGVSGGIG FADRRKDAGL 301 AVFHERRTYG ΝDAYSYTΝGA RLYFΝRWQTP KWQTLSΞAEW GRLKΝTRRAR 351 SDΝTHLQISΝ ΞLVFYRΝARQ YWMGGLDFYR ERΝPADRGDΝ FΝRYGLRFAW 401 GQEWGGSGLS ΞLLRLGAAKR HYEKPGFFSG FKGERRRDKE LΝTSLSLWHR 451 ALHFKGITPR LTLSHRETRS ΝDVFΝEYEKΝ RAFVEFΝKTF * Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 769 shows 99.8% identity over a 490 aa overlap with a predicted ORF (ORF 769) from N meningitidis: 769/a769 99.8% identity in 490 aa overlap 10 20 30 40 50 60 a769.pep LIMVIFYFCGKTFMPARΝRWMLLLPLLASAAYAEETPREPDLRSRPEFRLHEAEVKPIDR I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m769 LIMVIFYFCGKTFMPARΝRWMLLLPLLASAAYAEETPREPDLRSRPEFRLHEAEVKPIDR 10 20 30 40 50 60 70 80 90 100 110 120 a769.pep EKVPGQVREKGKVLQIDGETLLKΝPELLSRAMYSAVVSΝΝIAGIRVILPIYLQQAQQDKM I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m769 EKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGIRVILPIYLQQAQQDKM 70 80 90 100 110 120 130 140 150 160 170 180 a769.pep LALYAQGILAQADGRVKEAISHYRELIVAQPDAPAVRMRLAAALFENRQNEAAADQFDRL I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m769 LALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAAALFENRQNEAAADQFDRL 130 140 150 160 170 180 190 200 210 220 230 240 a769.pep KAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNINQAPKRQQYGKWTFPKQVDG I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m769 KAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNINQAPKRQQYGKWTFPKQVDG 190 200 210 220 230 240 250 260 270 280 290 300 a769.pep TAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKFNDMTAGVSGGIGFADRRKDAGL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m769 TAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKFNDMTAGVSGGIGFADRRKDAGL 250 260 270 280 290 300 310 320 330 340 350 360 a769.pep AVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLSSAEWGRLKNTRRARSDNTHLQISN I II I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m769 AVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLSSAEWGRLKNTRRARSDNTHLQISN 310 320 330 340 350 360 370 380 390 400 410 420 a769.pep SLVFYRNARQYWMGGLDFYRERNPADRGDNFNRYGLRFAWGQEWGGSGLSSLLRLGAAKR I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m769 SLVFYRNARQYWMGGLDFYRERNPADRGDNFNRYGLRFAWGQEWGGSGLSSLLRLGAAKR 370 380 390 400 410 420 430 440 450 460 470 480 a769.pep HYEKPGFFΞGFKGERRRDKELNTSLSLWHRALHFKGITPRLTLSHRETRSNDVFNEYEKN I I I I I II I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m769 HYEKPGFFSGFKGERRRDKELNTSLSLWHRALHFKGITPRLTLSHRETRSNDVFNEYEKN 430 440 450 460 470 480 490 a769.pep RAFVEFNKTFX I II I I I I I II I m769 RAFVEFNKTFX 490 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2645>: g770.seq 1 ATGAACAGAC TGCTACTGCT GTCTGCCGCC GTCCTGCCGA CTGCCTGCGG 51 CAGCGGCGAA ACCGATAAAA TCGGACGGGC AAGTACCGTT TTCAACATGT 101 TGGGCAAAAA CGACCGTATC GAAGTGGAAG GATTCGACGA TCCCGACGTT 151 CAAGGGGTTG CCTGTTATAT TTCGTATGCA AAAAAAGGCG GCTTGAAGGA 201 AATGGTCAAT TTGGAAGAGG ACGCGTCCGA CGCATCGGTT TCGTGCGTTC 251 AGACGGCATC TTCGATTTCT TTTGACGAAA CCGCCGTGCG CAAACCGAAA 301 GAAGTTTTCA AGCGCGGTAC GGGCTTCGCG TTCAAGAGCC GGCAGATTGT 351 CCGTTATTAC GACCCCAAAC GCAAAGCCTT CGCCTATTTG GTTTACAGCG 401 ATAAAATCGT CCAAGGATCG CCGAAAAATT CCTTAAGCGC GGTTTCCTGT 451 TTCGGCAGCG GCATACCGCA AACCGACGGG GTGCAAGCCG ATACTTCCGG 501 CAAACTGCTT GCCGGCGCCT GCATTATTTC CAACCCGATA AAAAATCCCG 551 ACAAACGCTG A This corresponds to the amino acid sequence <SEQ ID 2646; ORF 770.ng>: g770.pep 1 MΝRLLLLSAA VLPTACGSGE TDKIGRASTV FΝMLGKΝDRI EVEGFDDPDV 51 QGVACYISYA KKGGLKEMVΝ LEEDASDASV ΞCVQTASSIS FDETAVRKPK 101 EVFKRGTGFA FKSRQIVRYY DPKRKAFAYL VYSDKIVQGS PKΝSLΞAVSC 151 FGΞGIPQTDG VQADTSGKLL AGACIISΝPI KΝPDKR* The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 2647>: m770.seq 1 ATGAACAGAC TGCTACTGCT GTCTGCCGCC GTCCTGCTGA CTGCCTGCGG 51 CAGCGGCGAA ACCGATAAAA TCGGACGGGC AAGTACCGTT TTCAACATAC 101 TGGGCAAAAA CGACCGTATC GAAGTGGAAG GATTCGACGA TCCCGACGTT 151 CAAGGGGTTG CCTGTTATAT TTCGTATGCA AAAAAAGGCG GCTTGAAGGA 201 AATGGTCAAT TTGGAAGAGG ACGCGTCCGA CGCATCGGTT TCGTGCGTTC 251 AGACGGCATC TTCGATTTCT TTTGACGAAA CCGCCGTGCG CAAACCGAAA 301 GAAGTTTTCA AACACGGTGC GAGCTTCGCG TTCAAGAGCC GGCAGATTGT 351 CCGTTATTAC GACCCCAAAC GCAAAACCTT CGCCTATTTG GTGTACAGCG 401 ATAAAATCAT CCAAGGCTCG CCGAAAAATT CCTTAAGCGC GGTTTCCTGT 451 TTCGGCGGCG GCATACCGCA AACCGATGGG GTGCAAGCCG ATACTTCCGG 501 CAACCTGCTT GCCGGCGCCT GCATGATTTC CAACCCGATA GAAAATCTCG 551 ACAAACGCTG A This corresponds to the amino acid sequence <SEQ ID 2648; ORF 770>: m770.pep 1 MNRLLLLSAA VLLTACGSGE TDKIGRASTV FNILGKNDRI EVEGFDDPDV 51 QGVACYISYA KKGGLKEMVN LEEDASDASV SCVQTASSIS FDETAVRKPK 101 EVFKHGASFA FKSRQIVRYY DPKRKTFAYL VYSDKIIQGS PKNSLSAVSC 151 FGGGIPQTDG VQADTSGNLL AGACMISNPI ENLDKR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 770 shows 93.5% identity over a 186 aa overlap with a predicted ORF (ORF 770) from N gonorrhoeae m770/g770 93.5% identity in 186 aa overlap 10 20 30 40 50 60 g770.pep MΝRLLLLSAAVLPTACGSGETDKIGRASTVFΝMLGKΝDRIEVEGFDDPDVQGVACYISYA I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I m770 MΝRLLLLSAAVLLTACGSGETDKIGRASTVFΝILGKΝDRIEVEGFDDPDVQGVACYISYA 10 20 30 40 50 60 70 80 90 100 110 120 g770.pep KKGGLKEMVΝLEEDAΞDASVSCVQTASSISFDETAVRKPKEVFKRGTGFAFKSRQIVRYY I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I :: I I I I I I I I I I I I m770 KKGGLKEMVΝLEEDASDASVSCVQTASSISFDETAVRKPKEVFKHGAΞFAFKSRQIVRYY 70 80 90 100 110 120 130 140 150 160 170 180 g770.pep DPKRKAFAYLVYSDKIVQGSPKΝSLSAVΞCFGSGIPQTDGVQADTSGKLLAGACIIΞΝPI I I I I Ml I I I I I I I I Ml I I I I I II I I I I I I Ml I I I I I I I I I I I I Ml I I I I IM I I I I m770 DPKRKTFAYLVYSDKIIQGSPKΝΞLSAVSCFGGGIPQTDGVQADTSGΝLLAGACMIΞΝPI 130 140 150 160 170 180 g770.pep KΝPDKRX M llll ra770 EΝLDKRX The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2649>: a770.seq 1 ATGAACAGAC TGCTACTGCT GTCTGCCGCC GTCCTGCTGA CTGCCTGCGG 51 CAGCGGCGAA ACCGATAAAA TCGGACGGGC AAGTACCGTT TTCAACATAC 101 TGGGCAAAAA CGACCGTATC GAAGTGGAAG GATTCGACGA TCCCGACGTT 151 CAAGGGGTTG CCTGTTATAT TTCGTATGCA AAAAAAGGCG GCTTGAAGGA 201 AATGGTCAAT TTGGAAGAGG ACGCGTCCGA CGCATCGGTT TCGTGCGTTC 251 AGACGGCATC TTCGATTTCT TTTGACGAAA CCGCCGTGCG CAAACCGAAA 301 GAAGTTTTCA AACACGGTGC GAGCTTCGCG TTCAAGAGCC GGCAGATTGT 351 CCGTTATTAC GACCCCAAAC GCAAAACCTT CGCCTATTTG GTGTACAGCG 401 ATAAAATCAT CCAAGGCTCG CCGAAAAATT CCTTAAGCGC GGTTTCCTGT 451 TTCGGCGGCG GCATACCGCA AACCGATGGG GTGCAAGCCG ATACTTCCGG 501 CAACCTGCTT GCCGGCGCCT GCATGATTTC CAACCCGATA GAAAATCCCG 551 ACAAACGCTG A This corresponds to the amino acid sequence <SEQ ID 2650; ORF 770.a>: a770.pep 1 MΝRLLLLSAA VLLTACGSGE TDKIGRASTV FΝILGKΝDRI EVEGFDDPDV 51 QGVACYISYA KKGGLKEMVΝ LEEDASDASV SCVQTASSIS FDETAVRKPK 101 EVFKHGASFA FKSRQIVRYY DPKRKTFAYL VYSDKIIQGS PKNSLSAVSC 151 FGGGIPQTDG VQADTSGNLL AGACMISNPI ENPDKR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 770 shows 99.5% identity over a 186 aa overlap with a predicted ORF (ORF 770) from N meningitidis: m770/a770 99.5% identity in 186 aa overlap 10 20 30 40 50 60 a770.pep MΝRLLLLSAAVLLTACGSGETDKIGRASTVFΝILGKΝDRIEVEGFDDPDVQGVACYISYA I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I m770 MΝRLLLLSAAVLLTACGSGETDKIGRASTVFΝILGKΝDRIEVEGFDDPDVQGVACYISYA 10 20 30 40 50 60 70 80 90 100 110 120 a770.pep KKGGLKEMVΝLEEDASDASVSCVQTASSIΞFDETAVRKPKEVFKHGASFAFKSRQIVRYY I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m770 KKGGLKEMVΝLEEDASDASVSCVQTASSISFDETAVRKPKEVFKHGASFAFKSRQIVRYY 70 80 90 100 110 120 130 140 150 160 170 180 a770.pep DPKRKTFAYLVYSDKIIQGSPKΝSLSAVSCFGGGIPQTDGVQADTSGΝLLAGACMISΝPI I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I II II m770 DPKRKTFAYLVYSDKIIQGSPKΝSLSAVSCFGGGIPQTDGVQADTSGΝLLAGACMISΝPI 130 140 150 160 170 180 a770.pep EΝPDKRX II llll m770 EΝLDKRX The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 2651>: g771.seq 1 ATGGATTTAT TATCGGTTTT CCACAAATAC CGTCTGAAAT ATGCGGTGGC 51 GGTGCTGACG ATGCTGCTTT TGGCGGCAGT CGGGCTGCAC GCTTCCGTAT 101 ATCGCACCTT CACGCCCGAA AACATCCGCA GCCGCCTCCA ACAAAGCATT 151 GCCCATACCC ACCGGAAAAT CTCGTTTGAT GCGGATATAC GGCGCAGGCT 201 TCTGCCCCGC CCGACCGTCA TCCTGAAAAA CCTGACCATT ACCGAACCCG 251 ACGGCGGCCG GGTCGCCGTT TCCGTCAAAG AAACCAAAAT CGGATTGAGC 301 TGGAAAAACC TGTGGTCGGA TCGGATACAG GTTGAAAAAT GGGTGGTTTC 351 GGGTGCGGAT CTTGCCCTGA CGCGCGACAG AAACGGCGCT TGGAACATCC 401 AAGACCTGTT CGACGGCGCG AAACACTCCG CCTCAGTCAA CCGCATTATC 451 GTCGAAAACA GCACCGTCCG CCTCAATTTC CTGCAGCAAC AGCTTATCCT 501 GAAGGAAATC AGCCTCAACC TGCAATCCCC CGATTCGTCG GGGCAGCAGT 551 TTGAAAGTTC GGGCATACTG GTTTGGAGAA AGCTGTCCGT CCCGTGGAAA 601 AGCAGGGGGC TGTTCCTTTC AGACGGCATC GGCACGCCCG AAATCTCACC 651 GTTCCATTTT GAAGCTTCCA CTTCGCTGGA CGGACACGGC ATCACCATTT 701 CCACCACCGG CAGCCCTTCT GTCCGCTTCA ACGCCGGCGG AGCGGATGCC 751 GCCGGCCTCG GCCTGCGTGC AGACACTTCC TTCCGCAACC TCCACCTGAC 801 CGCGCAAATC CCCGCACTGG CACTCAAAAA CAACAGCATC AAAACCGGCA 851 CGGTCAACGG CACGTTTACC GCCGGCGGCG AATATGCCCG ATGGGACGGT 901 TCGTTCAAAC TCGACAAAGC CAACCTGCAC TCCGGCATCG CCAACATCGG 951 CAACGCCGAA ATCTCCGGCA GCTTCAAAAC ACCGCGCCTT CAAACCAATT 1001 TCTCCCTCGG CTCGCCGTTG GTTTGGAGTC GGGACAACGG GCTGGACGCC 1051 CCGCGCCTGC ACATATCGAC CCTTCAGGAT ACCGTCGACC GCCTGCCGCA 1101 ACCCCGTTTC ATCAGCCGGC TCGACGGTTC GCTGTCCATA CCGAATCTGC 1151 AAAATTGGAA TGCCGAATTA AACGGCACAT TCGACCGCCA ACCCGTTGCC 1201 GCAAAATTCA AATATACGCG GGAAGGCGCA CCGCACCTGG AAGCCGCCGC 1251 CGCGCTGCAA AAATTAAACC TCGCCCCCTA TCTTGACGAA TTTCGGCAAC 1301 AAAACGGCAA AATATTCCCC GACATCCTCG GCAGGCTGTC CGGCAACGTC 1351 GAGGCACACC TCAAAATCGG CAGCATCCAA CTCCCCGGCT TGCAACTGGA 1401 CGATATGGAA ACCTACCTCC ACGCCGACAA AGACCATATC GCGCTCAGCC 1451 GTTTCAAGTC AGGGCTTTAC GGCGGCCATA CCGAAGGCGG CATCAGCATC 1501 GCCAACACCC GTCCCGCCAC TTACCGCCTG CAACAGAATG CAAGCAACAT 1551 CCAAATCCAA CCGCTGCTGC AAGACCTGTT CGGCTTCCAC AGCTTCAGCG 1601 GCAACGGCGA TGCGGTCATC GACCTGACCG CAAGCGGCGA AAACCGCAAA 1651 CAGCTTATCC GCTCGCTGCA AGGCAGCCTG TCGCTGAATA TTTCCAACGG 1701 CGCGTGGCAC GGCATCGATA TGGACAGCAT TTTAAAAAAC GGCCTTTCCG 1751 GGAAAATCTC GGGCAGCACA CCCTTCTACC GATTCACGCT CAACAGCGAA 1801 ATTTCAGACG GCATCAGCCG CCACATCGAT ACCGAACTCT TCTCCGACAG 1851 CCTCTATGTT ACCAGCAACG GCTATACCAA TCTGGATACG CAGGAATTGT 1901 CTGAAGATGT CCTTATCCGC AACGCCGTCC ATCCGAAAAA CAAACCGATT 1951 CCCCTGAAAA TCACCGGTAC GGTGGACAAG CCGTCCATTA CCGTCGATTA 2001 CGGCAGGCTG ACCGGCGGCA TCAATTCGCG CAAAGAGAAA CAGAAAATCC 2051 TCGAAGACAC CCTGCTGGAA CAATGGCAGT GGCTCAAACC TAAAGAACCG 3051 TAA This corresponds to the amino acid sequence <SEQ ID 2652; ORF 771.ng>: g771 .pep 1 MDLLSVFHKY RLKYAVAVLT MLLLAAVGLH ASVYRTFTPE NIRSRLQQSI 51 AHTHRKISFD ADIRRRLLPR PTVILKNLTI TEPDGGRVAV SVKETKIGLS 101 WKNLWSDRIQ VEKWWSGAD LALTRDRNGA WNIQDLFDGA KHSASVNRII 151 VENSTVRLNF LQQQLILKEI SLNLQSPDSS GQQFESSGIL VWRKLSVPWK 201 SRGLFLSDGI GTPEISPFHF EASTSLDGHG ITISTTGSPS VRFNAGGADA 251 AGLGLRADTS FRNLHLTAQI PALALKNNSI KTGTVNGTFT AGGEYARWDG 301 SFKLDKANLH SGIANIGNAE ISGSFKTPRL QTNFSLGSPL VWSRDNGLDA 351 PRLHISTLQD TVDRLPQPRF ISRLDGSLSI PNLQNWNAEL NGTFDRQPVA 401 AKFKYTREGA PHLEAAAALQ KLNLAPYLDE FRQQNGKIFP DILGRLSGNV 451 EAHLKIGSIQ LPGLQLDDME TYLHADKDHI ALSRFKSGLY GGHTEGGISI 501 ANTRPATYRL QQNASNIQIQ PLLQDLFGFH SFSGNGDAVI DLTASGENRK 551 QLIRSLQGSL SLNISNGAWH GIDMDSILKN GLSGKISGST PFYRFTLNSE 601 ISDGISRHID TELFSDSLYV TΞNGYTNLDT QELSEDVLIR NAVHPKNKPI 65] PLKITGTVDK PSITVDYGRL TGGINSRKEK QKILEDTLLE QWQWLKPKEP 701 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2653>: m771.seq 1 ATGGATTTAT TATCGGTTTT CCACAAATAC CGTCTGAAAT ATGCGGTGGC 51 CGTGCTGACG ATACTGCTTT TGGCGGCAGT CGGGCTGCAC GCTTCCGTAT 101 ATCGCACCTT CACGCCTGAA AACATCCGCA GCCGCCTACA ACAAAGCATT 151 GCACACACAC ACCGGAAAAT CTCGTTTGAT GCGGACATTC AGCGCAGGCT 201 CCTGCCCCGG CCGACCGTCA TCCTGAAAAA CCTGACCATT ACCGAACCCG 251 GCGGCGACCA GACTGCCGTT TCCGTCCAAG AAACCAAAAT CGGATTGAGC 301 TGGAAAAACC TGTGGTCGGA TCAGATACAG ATTGAAAAAT GGGTGGTTTC 351 GAGTGCGGAA CTTGCCCTGA CGCGCGACGG GAAAGGTGTT TGGAACATCC 401 AAGACCTGAT CGACAGCCAA AAACGCCAAG CCTCAGTCAA CCGCATTATC 451 GTCGAAAACA GCACCGTCCG CCTCAATTTC CTGCAGGAAC AGCTTATCCT 501 GAAGGAAATC AACCTCAACC TGCAATCCCC CGATTCGTCG GGGCAGCCGT 551 TTGAAAGTTC GGGCATACTG GTTTGGGGAA AGCTGTCCGT CCCGTGGAAA 601 AGCAGGGGGC TGTTCCTTTC AAACGGCATC GGCCCGCCCG AAATCTCACC 651 GTTCCATTTT GAAGCTTCCA CTTCGCTGGA CGGACACGGC ATTACCATTT 701 CCACCACCGG CAGCCCTTCT GTCCGCTTCA ACGCCGGCGG AGCGGATGCC 751 GCCGGCCTCG GCCTGCGTGC AGACACTTCC TTCCGCAACC TCCACCTGAC 801 CGCCCAAATC CCCGCGCTGG CACTCAGGAA CAACAGCATT AAAATTGAAA 851 CCGTCAACGG CGCATTTACC GCCGGCGGCG AATATGCCCG ATGGGACGGT 901 TCGTTCAAAC TCGACAAAGC CAACCTGCAC TCCGGCATCG CCAACATCGG 951 CAACGCCGAA ATCTCCGGCA GCTTCAAAAC ACCGCGCCAC CAGACCAACT 1001 TCTCCCTCAA TTCGCCGCTC GTATGGACGG AAAACAAAGG GCTGGACGCG 1051 CCGCGCCTGT ATGTATCGAC CCTTCAGGAT ACCGTCAACC GCCTGCCGCA 1101 ACCCCGTTTC ATCAGCCGGC TCGACGGTTC GCTGTCCGTA CCGAATCTGC 1151 AAAATTGGAA TGCCGAATTA AACGGCACAT TCGACCGCCA AACCGTTGCC 1201 GCGAAATTCA GATACACACA TGAAGACGCA CCGCATCTGG AAGCCGCCGT 1251 CGCACTGCAA AAATTGAACC TGACCCCCTA TCTTGACGAC GTGCGGCAAC 1301 AAAACGGCAA AATATTTCCC GACACCCTCG CCAAGCTGTC CGGCGACATC 1351 GAGGCGCACC TGAAAATCGG AAAAGTCCAA CTTCCCGGCC TGCAACTGGA 1401 CGATATGGAA ACCTACCTCC ACGCCGACAA AGGCCATATC GCGCTCAGCC 1451 GTTTCAAGTC AGGGCTTTAC GGCGGCCATA CCGAAGGCGG CATCAGCATC 1501 GCCAACACCC GTCCCGCCAC TTACCGCCTG CAACAGAATG CAAGCAACAT 1551 CCAAATCCAA CCGCTGCTGC AAGACCTGTT CGGCTTCCAC AGCTTCAGCG 1601 GCAACGGCGA CGCGGTCATC GACCTGACCG CGGGCGGCGA AACCCGAAAA 1651 GAGCTTATCC GCTCGCTTCA GGGCAGCCTG TCGCTAAATA TTTCCAACGG 1701 TGCATGGCAC GGTATCGACA TGGACAATAT CCTGAAAAAC GGCATTTCGG 1751 GCAAAACTGC CGACAATGCC GCACCCAGCA CACCCTTCCA CCGATTCACG 1801 CTCAACAGCG AAATTTCAGA CGGCATCAGC CGCCACATCG ATACCGAACT 1851 CTTCTCCGAC AGCCTCTATG TTACCAGCAA CGGCTATACC AATCTGGATA 1901 CGCAGGAATT GTCTGAAGAT GTCCTTATCC GCAACGCCGT CCATCCGAAA 1951 AACAAACCGA TTCCCCTGAA AATCACCGGC ACGGTGGACA AACCGTCCAT 2001 TACCGTCGAT TACGGCAGGC TGACCGGCGG CATCAATTCG CGCAAAGAGA 2051 AACAGAAAAT CCTCGAAGAC ACCCTGCTGG AACAATGGCA GTGGCTCAAA 2101 CCTAAAGAAC CGTA This corresponds to the amino acid sequence <SEQ ID 2654; ORF 771>: m771.pep 1 MDLLSVFHKY RLKYAVAVLT ILLLAAVGLH ASVYRTFTPE NIRSRLQQSI 51 AHTHRKISFD ADIQRRLLPR PTVILKNLTI TEPGGDQTAV SVQETKIGLS 101 WKNLWSDQIQ IEKWWSSAE LALTRDGKGV WNIQDLIDSQ KRQASVNRII 151 VENSTVRLNF LQEQLILKEI NLNLQSPDSS GQPFESΞGIL VWGKLSVPWK 201 SRGLFLSNGI GPPEISPFHF EAΞTΞLDGHG ITISTTGSPS VRFNAGGADA 251 AGLGLRADTS FRNLHLTAQI PALALRNNSI KIETVNGAFT AGGEYARWDG 301 SFKLDKANLH SGIANIGNAE ISGSFKTPRH QTNFSLNSPL VWTENKGLDA 351 PRLYVSTLQD TVNRLPQPRF ISRLDGSLSV PNLQNWNAEL NGTFDRQTVA 401 AKFRYTHEDA PHLEAAVALQ KLNLTPYLDD VRQQNGKIFP DTLAKLSGDI 451 EAHLKIGKVQ LPGLQLDDME TYLHADKGHI ALSRFKSGLY GGHTEGGISI 501 ANTRPATYRL QQNASNIQIQ PLLQDLFGFH ΞFSGNGDAVI DLTAGGETRK 551 ELIRSLQGSL SLNISNGAWH GIDMDNILKN GISGKTADNA APSTPFHRFT 601 LNSEISDGIS RHIDTELFSD SLYVTSNGYT NLDTQELSED VLIRNAVHPK 651 NKPIPLKITG TVDKPSITVD YGRLTGGINS RKEKQKILED TLLEQWQWLK 701 PKEP* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 771 shows 90.3% identity over a 704 aa overlap with a predicted ORF (ORF 771) from N gonorrhoeae m771/g771 90.3% identity in 704 aa overlap 10 20 30 40 50 60 g771. ep MDLLSVFHKYRLKYAVAVLTMLLLAAVGLHASVYRTFTPEΝIRSRLQQSIAHTHRKISFD I I I I II I I II I I II I I I I I M I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m771 MDLLSVFHKYRLKYAVAVLTILLLAAVGLHASVYRTFTPEΝIRSRLQQSIAHTHRKISFD 10 20 30 40 50 60 70 80 90 100 110 120 g771.pep ADIRRRLLPRPTVILKΝLTITEPDGGRVAVSVKETKIGLSWKΝLWΞDRIQVEKWWSGAD I I MM I I I I I I I I I I I I I I I I I I :M I I M I I I I I I I I I I I I I I : I M I I I I I I : I : m771 ADIQRRLLPRPTVILKΝLTITEPGGDQTAVΞVQETKIGLSWKΝLWSDQIQIEKWWSSAE 70 80 90 100 110 120 130 140 150 160 170 180 g771.pep LALTRDRΝGAWΝIQDLFDGAKHΞAΞVΝRIIVEΝSTVRLΝFLQQQLILKEISLΝLQΞPDΞS llllll : Ml I I I I MM M : I I I I I I I I I I I I I I I I I I I : I I I I I I I : I I I I I I I I I m771 LALTRDGKGVWΝIQDLIDSQKRQASVΝRIIVEΝSTVRLΝFLQEQLILKEIΝLΝLQΞPDSS 130 140 150 160 170 180 190 200 210 220 230 240 g771.pep GQQFESSGILVWRKLSVPWKSRGLFLSDGIGTPEISPFHFEASTΞLDGHGITISTTGSPS II lllllllll I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II m771 GQPFESΞGILVWGKLSVPWKSRGLFLSΝGIGPPEISPFHFEAΞTSLDGHGITISTTGSPS 190 200 210 220 230 240 250 260 270 280 290 300 g771.pep VRFΝAGGADAAGLGLRADTSFRNLHLTAQIPALALKNNΞIKTGTVNGTFTAGGEYARWDG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I Ml I I I I I I I I : I I I I I I I I I I I I m771 VRFNAGGADAAGLGLRADTΞFRNLHLTAQIPALALRNNSIKIETVNGAFTAGGEYARWDG 250 260 270 280 290 300 310 320 330 340 350 360 g771.pep SFKLDKANLHSGIANIGNAEISGSFKTPRLQTNFSLGSPLVWSRDNGLDAPRLHISTLQD I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I Ml I I I I :::: I I I I I I I :: I I I I I m771 SFKLDKANLHSGIANIGNAEISGΞFKTPRHQTNFSLNSPLVWTENKGLDAPRLYVSTLQD 310 320 330 340 350 360 370 380 390 400 410 420 g771 . pep TVDRLPQPRFI SRLDGSLS I PNLQNWNAELNGTFDRQPVAAKFKYTREGAPHLEAAAALQ I I : I I I I I I I I I I I I I I I l I I I I I I I I I I I I I I I I I I I I I : I I : I I I I I I I I : I I I m771 TVNRLPQPRFISRLDGSLSVPNLQNWNAELNGTFDRQTVAAKFRYTHEDAPHLEAAVALQ 370 380 390 400 410 420 430 440 450 460 470 480 g771.pep KLNLAPYLDEFRQQNGKIFPDILGRLSGNVEAHLKIGSIQLPGLQLDDMETYLHADKDHI I M IM I I I : llllllllll M M I M M I I I I I I :: I I I I I I I I I I I I I I I I I I I m771 KLNLTPYLDDVRQQNGKIFPDTLAKLSGDIEAHLKIGKVQLPGLQLDDMETYLHADKGHI 430 440 450 460 470 480 490 500 510 520 530 540 g771.pep ALSRFKSGLYGGHTEGGISIANTRPATYRLQQNASNIQIQPLLQDLFGFHSFSGNGDAVI I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m771 ALSRFKSGLYGGHTEGGISIANTRPATYRLQQNASNIQIQPLLQDLFGFHSFSGNGDAVI 490 500 510 520 530 540 550 560 570 580 590 g771.pep DLTASGENRKQLIRSLQGSLSLNISNGAWHGIDMDSILKNGLSGKISG STPFYRFT I I I I M M I I M I I I I I I I I I I I I I I I I I I I I I I M I I I I M I I I : I I I I: I I I m771 DLTAGGETRKELIRSLQGSLSLNISNGAWHGIDMDNILKNGISGKTADNAAPSTPFHRFT 550 560 570 580 590 600 600 610 620 630 640 650 g771.pep LNSEISDGISRHIDTELFSDSLYVTSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITG II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m771 LNSEISDGISRHIDTELFSDSLYVTSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITG 610 620 630 640 650 660 660 670 680 690 700 g771.pep TVDKPSITVDYGRLTGGINSRKEKQKILEDTLLEQWQWLKPKEPX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m771 TVDKPSITVDYGRLTGGINSRKEKQKILEDTLLEQWQWLKPKEPX 670 680 690 700 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2655>: a771.seq 1 ATGGATTTAT TATCGGTCTT CCACAAATAC CGTCTGAAAT ATGCGGTAGC 51 CGTGCTGACG ATACTGCTTT TGGCGGCAAT CGGGCTGCAC GCTTCCGTAT 101 ATCGCATCTT CACACCTGAA AACATCCGAA GCCGCCTCCA ACAAAGCATT 151 GCCCATACGC ACCGGAAAAT CTCGTTTGAT GCGGATATAC AGCGCAGGCT 201 TCTGCCCCGG CCGACCGTCA TCCTGAAAAA CCTGACCATT ACCGAACCCG 251 GCGGCGACCG GACTGCCGTT TCCGTCCAAG AAACCAAAAT CGGATTGAGC 301 TGGAAAAACC TGTGGTCGGA TCAGATACAG ATTGAAAAAT GGGTGGTTTC 351 GAGTGCGGAA CTTGCCCTGA CGCGCGACGG GAAAGGTGTT TGGAACATCC 401 AAGACCTGAT CGACAGCCAA AAACGCCAAG CCTCAGTCAA CCGCATTATC 451 GTCGAAAACA GCACCGTCCG CCTCAATTTC CTGCAGGAAC AGCTTATCCT 501 GAAGGAAATC AACCTCAACC TGCAATCCCC CGATTCGTCG GGGCAGCCGT 551 TTGAAAGTTC GGGCATACTG GTTTGGGGAA AGCTGTCCGT CCCGTGGAAA 601 AGCAGGGGGC TGTTCCTTTC AGACGGCATC GGCACGCCCA AAATCTCACC 651 GTTCCATTTT GAAGCTTCCA CTTCGCTGGA CGGACACGGC ATTACCATTT 701 CCACCACCGG CAGCCCTTCT GTCCGCTTCA ACGCCGGCGG AGCGGATGCC 751 GCCGGCCTCG GCCTGCGTGC AGACACTTCC TTCCGCAACC TCCACCTGAC 801 CGCCCAAATC CCTACGCTGG CACTCAGGAA CAACAGCATT AAAATTGAAA 851 CCGTCAACGG CGCATTTACC GCCGGCGGCG AATATGCCCA ATGGGACGGT 901 TCGTTCAAAC TCGACAAAGC CAACCTGCAC TCCGGCATCG CCAACATCGG 951 CAACGCCGAA ATCTCCGGCA GCTTCAAAAC ACCGCGCCAC CAGACCAACT 1001 TCTCCCTCAA TTCGCCGCTC GTATGGACGG AAAACAAAGG GCTGGACGCG 1051 CCGCGCCTGT ATGTATCGAC CCTTCAGGAT ACCGTCAACC GCCTGCCGCA 1101 ACCCCGTTTC ATCAGCCGGC TCGACGGTTC GCTGTCCGTA CCGAATCTGC 1151 AAAATTGGAA TGCCGAATTA AACGGCACAT TCGACCGCCA AACCGTTGCC 1201 GCGAAATTCA GATACACACA TGAAGACGCA CCGCATCTGG AAGCCGCCGT 1251 CGCACTGCAA AAATTGAACC TGACCCCCTA TCTTGACGAC GTGCGGCAAC 1301 AAAACGGCAA AATATTTCCC GACACCCTCG CCAAGCTGTC CGGCGACATC 1351 GAGGCGCACC TGAAAATCGG AAAAGTCCAA CTTCCCGGCC TGCAACTGGA 1401 CGATATGGAA ACCTACCTCC ACGCCGACAA AGGCCATATC GCGCTCAGCC 1451 GTTTCAAGTC AGGGCTTTAC GGCGGCCATA CCGAAGGCGG CATCAGCATC 1501 GCCAACACCC GTCCCGCCAC TTACCGCCTG CAACAGAATG CAAGCAACAT 1551 CCAAATCCAA CCGCTGCTGC AAGACCTGTT CGGCTTCCAC AGCTTCAGCG 1601 GCAACGGCGA CGCGGTCATC GACCTGACCG CGGGCGGCGA AACCCGAAAA 1651 GAGCTTATCC GCTCGCTTCA GGGCAGCCTG TCGCTAAATA TTTCCAACGG 1701 TGCATGGCAC GGTATCGACA TGGACAATAT CCTGAAAAAC GGCATTTCGG 1751 GCAAAACTGC CGACAATGCC GCACCCAGCA CACCCTTCCA CCGATTCACG 1801 CTCAACAGCG AAATTTCAGA CGGCATCAGC CGCCACATCG ATACCGAACT 1851 CTTCTCCGAC AGCCTCTATG TTACCAGCAA CGGCTATACC AATCTGGATA 1901 CGCAGGAATT GTCTGAAGAT GTCCTTATCC GCAACGCCGT CCATCCGAAA 1951 AACAAACCGA TTCCCCTGAA AATCACCGGT ACGGTGGACA AACCGTCCAT 2001 TACCGTCGAT TACGGCAGGC TGACCGGCGG CATCAATTCG CGCAAAGAGA 2051 AACAGAAAAT CCTCGAAGAC ACCCTGCTGG AACAATGGCA GTGGCTCAAA 2101 CCTAAAGAAC CGTAA This corresponds to the amino acid sequence <SEQ ID 2656; ORF 771. a>: a771.pep 1 MDLLSVFHKY RLKYAVAVLT ILLLAAIGLH ASVYRIFTPE NIRSRLQQSI 51 AHTHRKISFD ADIQRRLLPR PTVILKNLTI TEPGGDRTAV SVQETKIGLS 101 WKNLWSDQIQ IEKWVVSSAE LALTRDGKGV WNIQDLIDSQ KRQASVNRII 151 VENSTVRLNF LQEQLILKEI NLNLQSPDSS GQPFESSGIL VWGKLSVPWK 201 ΞRGLFLΞDGI GTPKISPFHF EASTSLDGHG ITISTTGSPS VRFNAGGADA 251 AGLGLRADTS FRNLHLTAQI PTLALRNNSI KIETVNGAFT AGGEYAQWDG 301 SFKLDKANLH SGIANIGNAE ISGΞFKTPRH QTNFSLNSPL VWTENKGLDA 351 PRLYVΞTLQD TVNRLPQPRF ISRLDGSLSV PNLQNWNAEL NGTFDRQTVA 401 AKFRYTHEDA PHLEAAVALQ KLNLTPYLDD VRQQNGKIFP DTLAKLSGDI 451 EAHLKIGKVQ LPGLQLDDME TYLHADKGHI ALSRFKSGLY GGHTEGGISI 501 ANTRPATYRL QQNASNIQIQ PLLQDLFGFH SFSGNGDAVI DLTAGGETRK 551 ELIRSLQGSL SLNISNGAWH GIDMDNILKN GISGKTADNA APSTPFHRFT 601 LNSEISDGIS RHIDTELFΞD SLYVTSNGYT NLDTQELSED VLIRNAVHPK 651 NKPIPLKITG TVDKPSITVD YGRLTGGINS RKEKQKILED TLLEQWQWLK 701 PKEP* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 771 shows 98.9% identity over a 704 aa overlap with a predicted ORF (ORF 771) from N meningitidis m771/a771 98.9% identity in 704 aa overlap 10 20 30 40 50 60 a771.pep MDLLSVFHKYRLKYAVAVLTILLLAAIGLHASVYRIFTPEΝIRSRLQQSIAHTHRKISFD I I I I I I I I I I I I I I I I I I I I I I I I I M I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m771 MDLLSVFHKYRLKYAVAVLTILLLAAVGLHASVYRTFTPEΝIRSRLQQSIAHTHRKISFD 10 20 30 40 50 60 70 80 90 100 110 120 a771.pep ADIQRRLLPRPTVILKΝLTITEPGGDRTAVSVQETKIGLSWKΝLWSDQIQIEKWVVSSAE I I I I I I I I I I I I I II I I I I I I I I I I Ml I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M m771 ADIQRRLLPRPTVILKΝLTITEPGGDQTAVSVQETKIGLSWKΝLWSDQIQIEKWWΞSAE 70 80 90 100 110 120 130 140 150 160 170 180 a771.pep LALTRDGKGVWΝIQDLIDSQKRQASVΝRIIVEΝSTVRLΝFLQEQLILKEIΝLΝLQSPDSS I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I m771 LALTRDGKGVWΝIQDLIDSQKRQASVΝRIIVEΝSTVRLΝFLQEQLILKEIΝLΝLQSPDSS 130 140 150 160 170 180 190 200 210 220 230 240 a771.pep GQPFESSGILVWGKLSVPWKSRGLFLSDGIGTPKISPFHFEASTSLDGHGITISTTGSPS I I I I II I I I I I I I I I I I I I I I I I I I I I M I I Ml I I I I I I I I I I I I I I I I I I I I II I I I m771 GQPFEΞSGILVWGKLSVPWKSRGLFLSΝGIGPPEISPFHFEASTSLDGHGITISTTGSPS 190 200 210 220 230 240 250 260 270 280 290 300 a771.pep VRFΝAGGADAAGLGLRADTSFRΝLHLTAQIPTLALRΝΝSIKIETVΝGAFTAGGEYAQWDG I II I I I I I I I I I I I I I I I I I I I I I I I I I I I Ml I I I I I I I I I I I I I I I I I I I I I I I : I I I m771 VRFΝAGGADAAGLGLRADTSFRΝLHLTAQIPALALRΝΝSIKIETVΝGAFTAGGEYARWDG 250 260 270 280 290 300 310 320 330 340 350 360 a771.pep SFKLDKAΝLHSGIAΝIGΝAEISGSFKTPRHQTΝFSLΝSPLVWTEΝKGLDAPRLYVSTLQD I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m771 SFKLDKAΝLHΞGIAΝIGΝAEISGSFKTPRHQTΝFSLΝSPLVWTEΝKGLDAPRLYVSTLQD 310 320 330 340 350 360 370 380 390 400 410 420 a771.pep TVΝRLPQPRFISRLDGSLSVPΝLQΝWΝAELΝGTFDRQTVAAKFRYTHEDAPHLEAAVALQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I II I I I I m771 TVΝRLPQPRFISRLDGSLSVPΝLQΝWΝAELΝGTFDRQTVAAKFRYTHEDAPHLEAAVALQ 370 380 390 400 410 420 430 440 450 460 470 480 a771.pep KLΝLTPYLDDVRQQΝGKIFPDTLAKLSGDIEAHLKIGKVQLPGLQLDDMETYLHADKGHI II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I II I I I I M m771 KLΝLTPYLDDVRQQΝGKIFPDTLAKLSGDIEAHLKIGKVQLPGLQLDDMETYLHADKGHI 430 440 450 460 470 480 490 500 510 520 530 540 a771.pep ALSRFKSGLYGGHTEGGISIANTRPATYRLQQNASNIQIQPLLQDLFGFHSFSGNGDAVI I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II m771 ALSRFKSGLYGGHTEGGISIANTRPATYRLQQNASNIQIQPLLQDLFGFHSFSGNGDAVI 490 500 510 520 530 540 550 560 570 580 590 600 a771.pep DLTAGGETRKELIRSLQGSLSLNISNGAWHGIDMDNILKNGISGKTADNAAPSTPFHRFT I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II m771 DLTAGGETRKELIRSLQGSLSLNIΞNGAWHGIDMDNILKNGISGKTADNAAPSTPFHRFT 550 560 570 580 590 600 610 620 630 640 650 660 a771.pep LNSEISDGISRHIDTELFSDSLYVTSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I II I m771 LNSEISDGISRHIDTELFSDSLYVTSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITG 610 620 630 640 650 660 670 680 690 700 a771.pep TVDKPSITVDYGRLTGGINSRKEKQKILEDTLLEQWQWLKPKEPX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m771 TVDKPSITVDYGRLTGGINSRKEKQKILEDTLLEQWQWLKPKEPX 670 680 690 700 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2657>: g772.seq 1 GTGTTCGGCA CGGTCTTGCG GACTGATGCC GACTGCCTGC AAATCATCGT 51 CGTCGGCAAG TTCTTTCAGG TTGTTGCGTA TGGTTTTGCG GCGTTGGCGG 101 AAGGCGAGTT TCACCAGTTT GGCGAAATGA TCGAAATCGT CCGCCTTGCC 151 GATACGGTGT TTCACCGGAA TCATGCGCAC CACTGCGGAA TCGATTTTCG 201 GCGCGGGATC GAACGATTCG GGCGGCACGT CAATCAGCAG CTCCATATCG 251 AAAAAATATT GCAGCATCAC ACCCAAGCGA CCGTAGTCGT TGCTTTTCGG 301 CGCGGCAACC ATGCGCTCGA CCACTTCTTT TTGCAACATA AAGTGCATAT 351 CGGCGACATC GTCCGCCACC TCCGCCAGTT TGAACAAAAG CGGCGTGGAG 401 ATGTTATACG GCAGGTTGCC GACGATTTTC TTTTTGCCTG AGATGCCGTT 451 GAAATCAAAC TGCAACACGT CGCCTTCGTG AATCACCAGT TTATCCGCAA 501 ACGGCAGCGT TTTCAGACGG CATACGATGT CGCGGTCGAT TTCGACAACG 551 TGCAGGCGGT TCAGCTTTTT CGCCAAAGGT TCGGTAATTG CCGCCAAACC 601 CGGGCCGATT TCAATCACGA CATCATCCGC CTGCGGGCGC ACGGCGTTGA 651 CAATATCGCC GATAATCCGC GTGTCCTGCA AAAAATTCTG CCCGAAACGC 701 TTGCGGGCTT TGTGTTCTTT CATCGTGTTT CCTCTTCGGT TGAAACCCCG 751 CCCTTTAGGG CGGCAGGATC AGACTCTGTT TGGGCGGGGC GTAACCCCTT 801 CCAAATCAGG ACGACACATA GGGCGGTGCT TTATGTGTCG TCCTGTGTGT 851 TGGAACATAA ATGTGTTTAC AGTATCCGTT TGATGTCGGC ATTGTAA This corresponds to the amino acid sequence <SEQ ID 2658; ORF 772.ng>: g772.pep 1 VFGTVLRTDA DCLQIIWGK FFQWAYGFA ALAEGEFHQF GEMIEIVRLA 51 DTVFHRΝHAH HCGIDFRRGI ERFGRHVΝQQ LHIEKILQHH TQATVVVAFR 101 RGΝHALDHFF LQHKVHIGDI VRHLRQFEQK RRGDVIRQVA DDFLFA*DAV 151 EIKLQHVAFV ΝHQFIRKRQR FQTAYDVAVD FDΝVQAVQLF RQRFGΝCRQT 201 RADFΝHDIIR LRAHGVDΝIA DΝPRVLQKIL PETLAGFVFF HRVSSSVETP 251 PFRAAGSDSV WAGRΝPFQIR TTHRAVLYVS SCVLEHKCVY SIRLMΞAL* The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 2659>: m772.seq 1 ATGTTCGGCG CGGTCTTGCG GATTGATGCC GACTGCCTGC AAATCATCGT 51 CGCCTGCAAG CTCTTTCAGA TTGTTGCGTA TGGTTTTGCG GCGTTGGTGG 101 AAGGCGAGTT TCACGAGTTT GGCAAAATGC TCGAAATCGT CCGCCTTGCC 151 GATGCGGTGT TTCACCGGAA TCATACGGAC GACGGCGGAA TCCACTTTCG 201 GCGCAGGGTC GAACGATTCG GGCGGTACGT CAATCAGCAT TTCCATATCG 251 AAAAAATATT GCAGCATCAC GCCCAAGCGG CCGTAGTCGT TGCTTTTCGG 301 CGCGGCAACC ATACGCTCGA CCACTTCTTT TTGCAGCATA AAGTGCATAT 351 CGACGACATC GTCCGCCACC TCCGCCAGCT TGAACAAAAG CGGTGTGGAA 401 ATGTTGTACG GGAGGTTGCC GACGATTTTC TTTTTGCCTG CGATGCCGTT 451 GAAATCAAAC TGCAATACAT CGCCTTCGTG AATCACCAGT TTATCCGCAA 501 ACGGCAGCGT TTTCAGACGG CATACGATGT CGCGGTCGAT TTCGACAACG 551 TGCAGGCGGT TCAGCTTTTT CGCCAAAGGT TCGGTAATCG CCGCCAAACC 601 CGGGCCGATT TCAATCACGA CATCATCCGC CTGCGGGCGC ACGGCGTTGA 651 CAATATCGCT GATAATCCGC GTGTCCTGCA AAAAATTCTG CCCGAAACGC 701 TTGCGGGCTT TGTGTTCTTT CATCGTGTTT CCTTTTCGGT TGAAACCCCG 751 CCCTTTAGGG CGGTAGAATC AGACTCTATT TGGGAGGGGC GTAACTCTTT 801 CCAAATCAGG ATGGCACATA GGGCGGTGCT TTATGTGTCG TCCTGTGTGT 851 TGAAACATAA ATGTGTTTAC AGTATCCGTT TGATGTCGGC ATTGTAA This corresponds to the amino acid sequence <SEQ ID 2660; ORF 772>: m772.pep 1 MFGAVLRIDA DCLQIIVACK LFQIVAYGFA ALVEGEFHEF GKMLEIVRLA 51 DAVFHRNHTD DGGIHFRRRV ERFGRYVNQH FHIEKILQHH AQAAVWAFR 101 RGNHTLDHFF LQHKVHIDDI VRHLRQLEQK RCGNVVREVA DDFLFACDAV 151 EIKLQYIAFV NHQFIRKRQR FQTAYDVAVD FDNVQAVQLF RQRFGNRRQT 201 RADFNHDIIR LRAHGVDNIA DNPRVLQKIL PETLAGFVFF HRVSFSVETP 251 PFRAVESDSI WEGRNSFQIR MAHRAVLYVS SCVLKHKCVY SIRLMSAL* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 772 shows 85.2% identity over a 298 aa overlap with a predicted ORF (ORF 772) from N gonorrhoeae m772/g772 85 . 2 % identity in 298 aa overlap 10 20 30 40 50 60 g772 . pep VFGTVLRTDADCLQI IWGKFFQWAYGFAALAEGEFHQFGEMIEIVRLADTVFHRΝHAH M I M I I I I I I I I I I M M I M I I I I I I I M I I I I I : I I : M I I I I I I M I I I I I M m772 MFGAVLRIDADCLQIIVACKLFQIVAYGFAALVEGEFHEFGKMLEIVRLADAVFHRΝHTD 10 20 30 40 50 60 70 80 90 100 110 120 g772. ep HCGIDFRRGIERFGRHVΝQQLHIEKILQHHTQATVWAFRRGΝHALDHFFLQHKVHIGDI II III M I I I Ml I MM I II I I I I Ml Ml I I I I I I I I IM I I I I I I I I I I I II m772 DGGIHFRRRVERFGRYVΝQHFHIEKILQHHAQAAWVAFRRGΝHTLDHFFLQHKVHIDDI 70 80 90 100 110 120 130 140 150 160 170 180 g772. ep VRHLRQFEQKRRGDVIRQVADDFLFAXDAVEIKLQHVAFVΝHQFIRKRQRFQTAYDVAVD I I I I I I M I I I M IMM I I I I I I I I I I I I I I M : I I I I I I I I I I I I I I I I I I I I I I I m772 VRHLRQLEQKRCGΝVVREVADDFLFACDAVEIKLQYIAFVΝHQFIRKRQRFQTAYDVAVD 130 140 150 160 170 180 190 200 210 220 230 240 g772.pep FDΝVQAVQLFRQRFGΝCRQTRADFΝHDIIRLRAHGVDΝIADΝPRVLQKILPETLAGFVFF II I I I I I I I I I I I I I I I I I I I I I I I I I I I II I II II I I I I I I I I I II I II I I I I I I I I I m772 FDΝVQAVQLFRQRFGΝRRQTRADFΝHDIIRLRAHGVDΝIADΝPRVLQKILPETLAGFVFF 190 200 210 220 230 240 250 260 270 280 290 299 g772. pep HRVSSΞVETPPFRAAGSDSVWAGRΝPFQIRTTHRAVLYVSSCVLEHKCVYSIRLMSALX llll I I II I I I I I: IMM III llll M I I I I I I I I I I I : I I I I I I I I I I I I I I m772 HRVSFSVETPPFRAVESDSIWEGRΝSFQIRMAHRAVLYVSSCVLKHKCVYSIRLMSALX 250 260 270 280 290 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2661>: a772.seq 1 ATGTTCGGCG CGGTCTTGCG GATTGATGCC GACTGCCTGC AAATCATCGT 51 CGCCTGCAAG CTCTTTCAGA TTGTTGCGTA TGGTTTTGCG GCGTTGGTGG 101 AAGGCGAGTT TCACGAGTTT GGCGAAATGC TCGAAATCGT CCGCCTTGCC 151 GATACGGTGT TTCACCGGAA TCATGCGGAC GACGGCCGAA TCCACTTTCG 201 GCGCGGGGTC GAACGATTCG GGCGGCACGT CAATCAGCAT TTCCATATCG 251 AAGAAATATT GCAGCATCAC GCCCAAGCGG CCGTAGTCGT TGCTTTTCGG 301 CGCGGCAACC ATACGATCGA CCACTTCTTT TTGCAGCATA AAGTGCATAT 351 CGACGACATC GTCCGCCACC TCCGCCAGCT TGAACAAAAG CGGCGTGGAA 401 ATGTTGTAGG GCAGGTTGCC GACGATTTTC TTTTTGCCTG CGATGCCGTT 451 GAAATCAAAC TGCAATACAT CGCCTTCGTG AATCACCAGT TTATCCGCAA 501 ACGGCAGCGT TTTCAGACGG CATACGATGT CGCGGTCGAT TTCGACAACG 551 TGCAGGCGGT TCAGCTTTTT CGCCAAAGGT TCGGTAATCG CCGCCAAACC 601 CGGACCGATT TCAATCACGA CATCATCCGC CTGCGGGCGC ACGGCGTTGA 651 CAATATCGCT GATAATCCGC GTGTCCTGCA AAAAATTCTG CCCGAAACGC 701 TTGCGGGCTT TGTGTTCTTT CATCGTGTTT CCTTTTCGGT TGAAACCCCG 751 CCCTTTAGGG CGGTAGAATC AGACTCTATT TGGGAGGGGC GTAACTCCTT 801 CCAAATCAGG ACGGCACATA GGGCGGTGCT TTATGTGTCG TCCTGTGTGT 851 TGAAACATAA ATGTGTTTAC AGTATCCGTT TGATGTCGGC ATTGTAA This corresponds to the amino acid sequence <SEQ ID 2662; ORF 772. a>: a772.pep 1 MFGAVLRIDA DCLQIIVACK LFQIVAYGFA ALVEGEFHEF GEMLEIVRLA 51 DTVFHRNHAD DGRIHFRRGV ERFGRHVNQH FHIEEILQHH AQAAVWAFR 101 RGNHTIDHFF LQHKVHIDDI VRHLRQLEQK RRGNWGQVA DDFLFACDAV 151 EIKLQYIAFV NHQFIRKRQR FQTAYDVAVD FDNVQAVQLF RQRFGNRRQT 201 RTDFNHDIIR LRAHGVDNIA DNPRVLQKIL PETLAGFVFF HRVSFSVETP 251 PFRAVESDSI WEGRNSFQIR TAHRAVLYVS SCVLKHKCVY SIRLMSAL* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 772 shows 95.6% identity over a 298 aa overlap with a predicted ORF (ORF 772) from N. meningitidis m772/a772 95.6% identity in 298 aa overlap 10 20 30 40 50 60 a772.pep MFGAVLRIDADCLQIIVACKLFQIVAYGFAALVEGEFHEFGEMLEIVRLADTVFHRΝHAD I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M I I I I I I I I I : I I I I I M I m772 MFGAVLRIDADCLQIIVACKLFQIVAYGFAALVEGEFHEFGKMLEIVRLADAVFHRΝHTD 10 20 30 40 50 60 70 80 90 100 110 120 a772.pep DGRIHFRRGVERFGRHVΝQHFHIEEILQHHAQAAVWAFRRGΝHTIDHFFLQHKVHIDDI II I I I I I I I I I I I : I I I I I I I M I I I I I I I I I I I I I I I I I I I IM I I I I I I I I I I I I I m772 DGGIHFRRRVERFGRYVΝQHFHIEKILQHHAQAAWVAFRRGΝHTLDHFFLQHKVHIDDI 70 80 90 100 110 120 130 140 150 160 170 180 a772. pep VRHLRQLEQKRRGΝWGQVADDFLFACDAVEIKLQYIAFVΝHQFIRKRQRFQTAYDVAVD I I I I I I I I I I I llll : I I I I II I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I π 772 VRHLRQLEQKRCGΝWREVADDFLFACDAVEIKLQYIAFVΝHQFIRKRQRFQTAYDVAVD 130 140 150 160 170 180 190 200 210 220 230 240 a772 . pep FDΝVQAVQLFRQRFGΝRRQTRTDFΝHDIIRLRAHGVDΝIADΝPRVLQKILPETLAGFVFF I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m772 FDΝVQAVQLFRQRFGΝRRQTRADFΝHDIIRLRAHGVDΝIADΝPRVLQKILPETLAGFVFF 190 200 210 220 230 240 250 260 270 280 290 299 a772.pep HRVSFSVETPPFRAVESDSIWEGRΝSFQIRTAHRAVLYVSSCVLKHKCVYSIRLMSALX I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m772 HRVSFSVETPPFRAVESDSIWEGRΝSFQIRMAHRAVLYVSSCVLKHKCVYSIRLMSALX 250 260 270 280 290 g773.seq not found yet g773.pep not found yet The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2663>: m773.seq 1 ATGGGATTGG GTGCAACGAC TTTTGTCGGT TCGGGTGCTA TAGGCGGAGG 51 TCTGTGCAGT ACCGGGATTG GCTGTGCGGC CGGTGGACTT ATTGCAACGG 101 CAGGTATGAC CGGTGGTTAT ACACAGGCCT CAGAAGGAAG CCGGCAATTG 151 TTTGGCACTT ACCAGTCCGA TTTTGGTAAA AAAGTTGTCC TATCTTTGGG 201 TACACCAATA GAATACGAAT CGCCGTTAGT ATCTGATGCG AAAAATCTAG 251 CCGTATGGGG ATTGGAAACG CTGATTACGC GCAAATTGGG AAACTTGGCA 301 ACGGGTGTGA AAACTTCCTT GACTCCGAAA ACTGCTGACG TACAGCGAAA 351 TATCCTGTCC CAATCCGAAG TCGGTATCAA GTGGGGCAAG GGGATTGAAG 401 GACAGGGAAT GCCTTGGGAG GATTATGTCG GTAAGGGCTT GTCTGCCAAT 451 GCAAGGTTAC CTAAAAATTT TAAAACATTT GATTATTTTG ATCGTGGTAC 501 AGGCACGGCA ATCAGTGCCA AAACTCTGGA TACGCAAACT ACGGCACGCC 551 TGTCCAAACC CGAACAGCTT TACAGTACCA TGAAAGGGTA CATCGATAAG 601 ACGGCAAATT TCAAAAGTTA TGAATTATCA GAAGTACCGT TAAGGGCAGA 651 CATGATCAAA CAGCGCGAAA TCCATCTGGC CATACCCGCA CAAACTAATA 701 AGGAGCAAAG ATTGCAGTTG CAACGTGTGG TAGAGTATGG CAAAAGTCAA 751 AACATTACAG TCAAAATTAC GGAGATCGAA TAA This corresponds to the amino acid sequence <SEQ ID 2664; ORF 773>: m773.pep 1 MGLGATTFVG SGAIGGGLCS TGIGCAAGGL IATAGMTGGY TQASEGSRQL 51 FGTYQSDFGK KVVLSLGTPI EYESPLVSDA KNLAVWGLET LITRKLGNLA 101 TGVKTSLTPK TADVQRNILΞ QSEVGIKWGK GIEGQGMPWE DYVGKGLSAN 151 ARLPKNFKTF DYFDRGTGTA ISAKTLDTQT TARLSKPEQL YSTMKGYIDK 201 TANFKSYELS EVPLRADMIK QREIHLAIPA QTNKEQRLQL QRVVEYGKSQ 251 NITVKITEIE * a773.seq not found yet a773.pep not found yet The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2665>: g774.seq 1 ATGAAGACCA AATTACCGCT TTTTATCATT TGGCTGTCTG TGTCTGCCTC 51 CTGTGCTTCC GTTTTACCCG TTCCGGAGGG CAGCCGAACC GAAATGCCGA 101 CACAGGAAAA TGCTTCAGAC GGCATTCCCT ATCCCGTTCC CACTCTGCAA 151 GACCGTTTGG ACTATCTGGA AGGCAAAATC GTCCGGCTGT CGAACGAAGT 201 GGAAATGTTA AACGGGAAAG TCAAAGCATT GGAGCATACG AAAATACACC 251 CTTCCGGCAG GACATACGTC CAAAAACTCG ACGACCGCAA ATTGAAAGAG 301 CATTACCTCA ATACCGAAGG CGGCAGCGCA TCCGCACATA CCGTCGAAAC 351 CGCACAAAAC CTCTACAATC AGGCACTCAA ACACTATCAA AACGGCAGGT 401 TTTCTGCCGC AGCCGCCTTG TTGAAGGGGG CGGACGGCGG AGACGGCGGC 451 AGCATCGCGC AACGCAGTAT GTACCTGTTG CTGCAAAGCA GGGCGCGTAT 501 GGGGAACTGT GAATCTGTCA TCGAAATCGG AGGGCGTTAC GCCAACCGTT 551 TCAAAGACAG CCCAACCGCG CCCGAAGTCA TATTCAAAAT CGGCGAATGC 601 CAATACAGGC TTCAGCAAAA AGACATTGCA AGGGCGACTT GGCGCAGCCT 651 GATACAGACC TATCCCGGCA GCCCGGCGGC AAAACGCGCC GCCGCAGCCG 701 TACGCAAACG ATAG This corresponds to the amino acid sequence <SEQ ID 2666; ORF 774.ng>: g774.pep 1 MKTKLPLFII WLSVSASCAS VLPVPEGSRT EMPTQEΝASD GIPYPVPTLQ 51 DRLDYLEGKI VRLSΝEVEML ΝGKVKALEHT KIHPSGRTYV QKLDDRKLKE 101 HYLΝTEGGΞA ΞAHTVETAQΝ LYΝQALKHYQ ΝGRFSAAAAL LKGADGGDGG 151 ΞIAQRSMYLL LQSRARMGΝC ESVIEIGGRY AΝRFKDSPTA PEVIFKIGEC 201 QYRLQQKDIA RATWRSLIQT YPGΞPAAKRA AAAVRKR* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2667>: m774. seq 1 ATGAAGATCA AATTACCGCT TTTTATCATT TGGCTGTCTG TGTCCGCCTC 51 CTGTGCTTCC GTTTCACCCG TTCCGGCAGG CAGCCAAACC GAAATGTCGA 101 CACGGGAAAA TGCTTCAGAC GGCATTCCCT ATCCCGTTCC GACCTTGCAA 151 GACCGTTTGG ACTATCTGGA AGGCAAAATC GTCCGGCTGT CGAACGAAGT 201 GGAAACCTTA AACGGCAAAG TCAAAGCACT GGAACACGCA AAAACACATT 251 CTTCCGGCAG GGCATACGTC CAAAAACTCG ACGACCGCAA GTTGAAAGAG 301 CATTACCTCA ATACCGAAGG CGGCAGCGCA TCCGCACATA CTGTCGAAAC 351 CGCACAAAAC CTCTACAATC AGGCACTCAA ACACTATAAA AGCGGCAAGT 401 TTTCTGCCGC TGCCTCCCTG TTGAAAGGCG CGGACGGAGG CGACGGCGGC 451 AGCATCGCGC AACGCAGTAT GTACCTGTTG CTGCAAAGCA GGGCGCGTAT 501 GGGCAACTGC GAATCCGTCA TCGAAATCGG AGGGCGTTAC GCCAACCGTT 551 TCAAAGACAG CCCAACCGCG CCTGAAGCCA TGTTCAAAAT CGGCGAATGC 601 CAATACAGGC TTCAGCAAAA AGACATTGCA AGGGCGACTT GGCGCAGCCT 651 GATACAGACC TATCCCGGCA GCCCGGCGGC AAAACGCGCC GCCGCAGCCG 701 TGCGCAAACG ATAG This corresponds to the amino acid sequence <SEQ ID 2668; ORF 774>: m774.pep 1 MKIKLPLFII WLSVSASCAS VSPVPAGSQT EMSTREΝASD GIPYPVPTLQ 51 DRLDYLEGKI VRLSΝEVETL ΝGKVKALEHA KTHSSGRAYV QKLDDRKLKE 101 HYLΝTEGGSA SAHTVETAQΝ LYΝQALKHYK SGKFΞAAASL LKGADGGDGG 151 SIAQRSMYLL LQSRARMGΝC ESVIEIGGRY AΝRFKDSPTA PEAMFKIGEC 201 QYRLQQKDIA RATWRSLIQT YPGSPAAKRA AAAVRKR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 774 shows 92.8% identity over a 237 aa overlap with a predicted ORF (ORF 774) from N gonorrhoeae m774/g774 92.8% identity in 237 aa overlap 10 20 30 40 50 60 g77 .pep MKTKLPLFIIWLSVSASCASVLPVPEGSRTEMPTQEΝASDGIPYPVPTLQDRLDYLEGKI II I I I I I I I I I I I I I I I I I I III I I M I I I : I I I I I I I I I I I I I I I I I I I I I I I I I m774 MKIKLPLFI IWLSVSASCASVSPVPAGSQTEMSTREΝASDGI PYPVPTLQDRLDYLEGKI 10 20 30 40 50 60 70 80 90 100 110 120 g774. pep VRLSΝEVEMLΝGKVKALEHTKIHPSGRTYVQKLDDRKLKEHYLΝTEGGSASAHTVETAQΝ llllllll I I I I I I I I I M I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m774 VRLSΝEVETLΝGKVKALEHAKTHSSGRAYVQKLDDRKLKEHYLΝTEGGSASAHTVETAQΝ 70 80 90 100 110 120 130 140 150 160 170 180 g77 .pep LYΝQALKHYQΝGRFSAAAALLKGADGGDGGSIAQRSMYLLLQSRARMGΝCESVIEIGGRY I I I I I I I I I :: I M I I I M I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m77 LYΝQALKHYKSGKFSAAASLLKGADGGDGGΞIAQRSMYLLLQSRARMGΝCESVIEIGGRY 130 140 150 160 170 180 190 200 210 220 230 g774.pep AΝRFKDSPTAPEVIFKIGECQYRLQQKDIARATWRSLIQTYPGSPAAKRAAAAVRKRX I I I I I I I I I I I MM I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m774 AΝRFKDSPTAPEAMFKIGECQYRLQQKDIARATWRSLIQTYPGSPAAKRAAAAVRKRX 190 200 210 220 230 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2669>: a774.seq 1 ATGAAGACCA AATTACCGCT TTTTATCATT TGGCTGTCCG TATCCGCCGC 51 CTGTTCTTCC CCTGTTTCCC GCAATATTCA GGATATGCGG CTCGAACCGC 101 AGGCAGAGGC AGGTAGTTCG GACGCTATTC CCTATCCCGT TCCCACTCTG 151 CAAGACCGTT TGGATTATCT GGAAGGCACA CTCGTCCGCC TGTCGAACGA 201 AGTGGAAACC TTAAACGGCA AAGTCAAAGC ACTGGAGCAT GCGAAAACAC 251 ACCCTTCCAG CAGGGCATAC GTCCAAAAAC TCGACGACCG CAAGTTGAAA 301 GAGCATTACC TCAATACCGA AGGCGGCAGC GCATCCGCAC ATACCGTCGA 351 AACCGCACAA AACCTCTACA ATCAGGCACT CAAACACTAT AAAAGCGGCA 401 GGTTTTCTGC CGCTGCCTCC CTGTTGAAAG GCGCGGACGG AGGCGACGGC 451 GGCAGCATCG CGCAACGCAG TATGTACCTG TTGCTGCAAA GCAGGGCGCG 501 TATGGGCAAC TGCGAATCCG TCATCGAAAT CGGAGGGCGT TACGCCAACC 551 GTTTCAAAGA CAGCCCAACC GCGCCTGAAG CCATGTTCAA AATCGGCGAA 601 TGCCAATACA GGCTTCAGCA AAAAGACATT GCAAGGGCGA CTTGGCGCAG 651 CCTGATACAG ACCTATCCCG GCAGCCCGGC GGCAAAACGC GCCGCCGCAG 701 CCGTGCGCAA ACGATAG This corresponds to the amino acid sequence <SEQ ID 2670; ORF 774.a>: a774.pep 1 MKTKLPLFII WLSVSAACSS PVSRΝIQDMR LEPQAEAGSS DAIPYPVPTL 51 QDRLDYLEGT LVRLSΝEVET LΝGKVKALEH AKTHPSSRAY VQKLDDRKLK 101 EHYLΝTEGGS ASAHTVETAQ ΝLYΝQALKHY KSGRFSAAAS LLKGADGGDG 151 GSIAQRSMYL LLQSRARMGΝ CESVIEIGGR YAΝRFKDSPT APEAMFKIGE 201 CQYRLQQKDI ARATWRSLIQ TYPGSPAAKR AAAAVRKR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 774 shows 89.5% identity over a 238 aa overlap with a predicted ORF (ORF 774) from N meningitidis m774/a774 89.5% identity in 238 aa overlap 10 20 30 40 50 60 a774.pep MKTKLPLFIIWLSVSAACSSPVSRΝIQDMRLEPQAEAGSSDAIPYPVPTLQDRLDYLEGT II II I I I I I I I I II IMM II : I ::: : : I M I I I I I I I I I I I I || | | I m774 MKIKLPLFIIWLSVSASCAS-VSPVPAGSQTEMSTREΝASDGIPYPVPTLQDRLDYLEGK 10 20 30 40 50 70 80 90 100 110 120 a774.pep LVRLSNEVETLNGKVKALEHAKTHPSSRAYVQKLDDRKLKEHYLNTEGGSASAHTVETAQ : I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I II II I I I I I I I I I I I I I I I I I II m774 IVRLSNEVETLNGKVKALEHAKTHSSGRAYVQKLDDRKLKEHYLNTEGGSASAHTVETAQ 60 70 80 90 100 110 130 140 150 160 170 180 a774.pep NLYNQALKHYKSGRFSAAASLLKGADGGDGGSIAQRSMYLLLQSRARMGNCESVIEIGGR I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m774 NLYNQALKHYKSGKFΞAAASLLKGADGGDGGSIAQRSMYLLLQSRARMGNCESVIEIGGR 120 130 140 150 160 170 190 200 210 220 230 239 a774.pep YANRFKDSPTAPEAMFKIGECQYRLQQKDIARATWRSLIQTYPGSPAAKRAAAAVRKRX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m774 YANRFKDΞPTAPEAMFKIGECQYRLQQKDIARATWRSLIQTYPGSPAAKRAAAAVRKRX 180 190 200 210 220 230 g790.seq not found yet g790.pep not found yet The following partial DNA sequence was identified in N meningitidis <SEQ ID 2671>: m790.seq 1 ATGGCAAGAA GGTCAAAAAC ATTTGAAGAA GCTGCTGCTG AGGTTGAGGA 51 ACGTTTCGGT CATCGTGGCA TTAAGTTGGT CGAGTTTGAG GGTACAGCCA 101 AGCCGTGTGT AATCAACTGC CCTAAACATG GAAACCAAAC CTGTTCGAGG 151 TACTCCAATA TGTTCATAGG AAGTAGCTGG GGTTGCCCCT CTTGTGGTAA 201 TGAGCAAGCT GCAAAAGCCG GTATAGCGAC CCTTAGGAAG AATCACATAG 251 CGTTAGAAAT GCTGAAACAG GCTGTAACAG GTATGACCAA GCAAGAGCGC 301 ATCACGACGC AAGCCTACAA TGAGATGACC AAATCCGTGG CAGGTTCAAA 351 CAGCATAGTC CTTAACGATG TCCAAGGCGA TACGACCATC AACAACCATC 401 ATACGCATAC GCACAACCAC AGCGATGCCG ATGGCAAAGC ACTGTCGATG 451 AGGCTCACAC CCCGTCCTTT GTTGTCAGAC CGTCAGGCGG CGGCTTTCGC 501 CCGTACAGGC AAACTCACGG GCAGTTTCGA CCTGTTTGCT TCGGTGgTCG 551 CCCCCTCGCA GTACACGTTT GCCGTTGCCA TGCCCGACAC GTCCATGTCG 601 CCGGTTATCG AAAAGGGAGA CTTGCTGGTG GTCGAGCCGC GTATGTGCCC 651 TGCGGACGAA GACATCGCGC TGATTGAACT GTCCGACAAG CGGCTGGTCG 701 TCGCGCACCT TGTTATCGAT ATTGCGGGCA GGATGCTGAT TTATCAGACG 751 GGCAGGCCGT CTGAAGCCTT TGACCTGCCC GAAGGCAGCA CGATTTTAGG 801 TGTGGTGCTG GAGTCAAAAA ACGGTTTATG TCCGCCGCAC AGGCAAGAAG 851 GCGTGTTGAT TCGGATTACC GCCCCTGATG TGTGGACGGT TGGTATGATT 901 TCCGCTTCCA AAACGTCGTG TACGCGCCCG ACCGCAGCCC GGAAATCAGC 951 CGTATGCTTT CTTCGATTTT GGCAGGCTAC GCGTGGGATA CCGAAAACCC 1001 GTTCGTGGCG AAATCCGAAC AACGCCTGA This corresponds to the amino acid sequence <SEQ ID 2672; ORF 790>: m790.pep 1 MARRΞKTFEE AAAEVEERFG HRGIKLVEFE GTAKPCVIΝC PKHGΝQTCSR 51 YΞΝMFIGSSW GCPSCGΝEQA AKAGIATLRK ΝHIALEMLKQ AVTGMTKQER 101 ITTQAYΝEMT KSVAGSΝSIV LΝDVQGDTTI ΝΝHHTHTHΝH SDADGKALSM 151 RLTPRPLLSD RQAAAFARTG KLTGΞFDLFA SWAPSQYTF AVAMPDTΞMS 201 PVIEKGDLLV VEPRMCPADE DIALIELΞDK RLVVAHLVID IAGRMLIYQT 251 GRPSEAFDLP EGSTILGWL EΞKΝGLCPPH RQEGVLIRIT APDVWTVGMI 301 SASKTΞCTRP TAARKSAVCF LRFWQATRGI PKTRSWRΝPΝ ΝA* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2673>: a790.seq 1 ATGGCAAGAA GGTCAAAAAC ATTTGAAGAA GCTGCTGCTG AGGTTGAGGA 51 ACGTTTCGGT CATCGTGGCA TTAAGTTGGT CGAGTTTGAG GGTACAGCCA 101 AGCCGTGTGT AATCAACTGC CCTAAACATG GAAACCAAAC CTGTTCGAGG 151 TACTCCAATA TGTTCATAGG AAGTAGCTGG GGTTGCCCCT CTTGTGGTAA 201 TGAGCAAGCT GCAAAAGCCG GTATAGCGAC CCTTAGGAAG AATCACATAG 251 CGTTAGAAAT GCTGAAACAG GCTGTAACAG GTATGACCAA GCAAGAGCGC 301 ATCACGACGC AAGCCTACAA TGAGATGACC AAATCCGTGG CAGGTTCAAA 351 CAGCATAATC CTTAACGATG TCCAAGGCGA TACGACCATC AACAACCATC 401 ATACGCATAC GCACAACCAC AGCGATGCCG ACGGCAAAGC ACTGTCGATG 451 AGGCTCACAC CCCGTCCTTT GTTGTCAGAC CGTCAGGCGG CGGCTTTCGC 501 CCGTACAGGC AAACTCACGG GCAGTTTCGA CCTGTTTGCT TCGGTGGTCG 551 CCCCTTCACA ATATACGTTT GCCGTTGCCA TGCCCGACAC GTCCATGTCG 601 CCGGTTATCG AAAAGGGGGA TTTGCTGGTG GTCGAGCCGC GTATGCGCCC 651 TGCGGACGAA GACATCGTAC TGATTGAACT GTCCGACAAG CGGCTGGTCG 701 TCGCGCACCT TGTTATCGAT ATTGCGGGCA GGATGCTGAT TTATCAGACG 751 GGCAGGCCGT CTGAAGCCCT CGACCTGCCC GAAGGCAGCG TGATTTTAGG 801 TGTGGTGCTG GAGTCAAAAA ACGGTTTATG TCCGCCGCAC AGGCAAGAAG 851 GCGTGTTGAT TCGGATTACC GCCCCTGATG TGTGGACGGT TGGTACGATT 901 TCCGCTTCCA AAACGTCGTG TACGCGCCCG ACCGCAGCCC GGAAATCAGC 951 CGTATGCTTT CTTCGATTTT GGCAGGCTAC GCGTGGGATA CCGAAAACCC 1001 GTTCGTGGCG AAATCCGAAC AACGCCTGT This corresponds to the amino acid sequence <SEQ ID 2674; ORF 790. a>: a790.pep 1 MARRSKTFEE AAAEVEERFG HRGIKLVEFE GTAKPCVINC PKHGNQTCSR 51 YSNMFIGSSW GCPSCGNEQA AKAGIATLRK NHIALEMLKQ AVTGMTKQER 101 ITTQAYNEMT KSVAGSNSII LNDVQGDTTI NNHHTHTHNH SDADGKALSM 151 RLTPRPLLSD RQAAAFARTG KLTGSFDLFA SWAPSQYTF AVAMPDTSMS 201 PVIEKGDLLV VEPRMRPADE DIVLIELSDK RLVVAHLVID IAGRMLIYQT 251 GRPSEALDLP EGSVILGWL ESKNGLCPPH RQEGVLIRIT APDVWTVGTI 301 SASKTΞCTRP TAARKSAVCF LRFWQATRGI PKTRSWRNPN NAC Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 790 shows 98.2% identity over a 342 aa overlap with a predicted ORF (ORF 790) from N. meningitidis a790/ 790 98.2% identity in 342 aa overlap 10 20 30 40 50 60 a790.pep MARRSKTFEEAAAEVEERFGHRGIKLVEFEGTAKPCVIΝCPKHGΝQTCΞRYSΝMFIGSSW I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I m790 MARRSKTFEEAAAEVEERFGHRGIKLVEFEGTAKPCVIΝCPKHGΝQTCSRYSΝMFIGSSW 10 20 30 40 50 60 70 80 90 100 110 120 a790.pep GCPSCGΝEQAAKAGIATLRKΝHIALEMLKQAVTGMTKQERITTQAYΝEMTKSVAGΞΝSII I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : m790 GCPSCGΝEQAAKAGIATLRKΝHIALEMLKQAVTGMTKQERITTQAYΝEMTKSVAGΞΝSIV 70 80 90 100 110 120 130 140 150 160 170 180 a790.pep LΝDVQGDTTIΝΝHHTHTHΝHSDADGKALSMRLTPRPLLSDRQAAAFARTGKLTGSFDLFA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I IT1790 LΝDVQGDTTIΝΝHHTHTHΝHSDADGKALSMRLTPRPLLSDRQAAAFARTGKLTGSFDLFA 130 140 150 160 170 180 190 200 210 220 230 240 a790 . pep ΞWAPSQYTFAVAMPDTSMSPVIEKGDLLWEPRMRPADEDIVLIELSDKRLVVAHLVID I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M l I I I I I I I I I I I I I I I I m790 SWAPSQYTFAVAMPDTSMSPVIEKGDLLWEPRMCPADEDIALIELSDKRLWAHLVID 190 200 210 220 230 240 250 260 270 280 290 300 a790 . pep IAGRMLIYQTGRPSEALDLPEGSVILGVVLESKΝGLCPPHRQEGVLIRITAPDVWTVGTI I I I I I I I I I I I I I I I M l I I I I M l I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m790 IAGRMLIYQTGRPSEAFDLPEGSTILGWLESKΝGLCPPHRQEGVLIRITAPDVWTVGMI 250 260 270 280 290 300 310 320 330 340 a790.pep SASKTSCTRPTAARKΞAVCFLRFWQATRGIPKTRSWRΝPΝΝAC I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I m790 SASKTΞCTRPTAARKSAVCFLRFWQATRGIPKTRSWRΝPΝΝAX 310 320 330 340 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 2675>: g791.seq 1 ATGGTAAATT ATTATTCAGC TATGATTAAA AAGATTTTAA CTACTTGTTT 51 TGGTTTGTTT TTTGGTTTTT GTGTATTTGG AGTGGGTCTG GTTGCCATTG 101 CTATTTTGGT AACGTATCCG AAACTGCCGT CTTTGGATTC TTTGCAGCAT 151 TACCAGCCTA AAATGCCGTT GACTATTTAT TCGGCGGATG GAGAAGTCAT 201 CGGTATGTAT GGGGAGCAGC GGCGCGAATT TACAAAAATC GGCGATTTCC 251 CCGAGGTGTT GCGGAATGCG GTTATTGCCG CCGAGGATAA ACGCTTTTAC 301 CGGCATTGGG GGGTGGATGT TTGGGGTGTT GCCCGCGCTG CCGTCGGCAA 351 TGTCGTGTCC GGCAGCGTGC AGTCGGGTGC GAGTACGATT ACACAGCAGG 401 TGGCGAAAAA TTTTTATTTG AGCAGTGAAA AAACGTTCAC ACGCAAATTC 451 AATGAGGTGT TGCTTGCCTA TAAAATCGAG CAGTCTTTAA GCAAAGACAA 501 AATCCTTGAG TTGTATTTCA ATCAGATTTA CCTCGGTCAG CGCGCCTATG 551 GTTTTGCATC TGCCGCGCAA ATCTATTTCA ATAAGAATGT CCGAGATTTG 601 ACTTTGGCGG AAGCCGCCAT GCTTGCGGGA CTGCCCAAGG CTCCGTCTGC 651 CTATAATCCG ATTGTTAATC CGGAGCGTGC CAAGTTGCGC CAGAAGTATA 701 TTTTGAACAA TATGCTCGAG GAGAAGATGA TTACCGTGCA ACAGCGCGAT 751 CAGGCATTGA ATGAGGAACT GCATTATGAG CGGTTTGTTC GGAAAATCGA 801 TCAGAGTGCT TTATATGTGG CGGAAATGGT GCGTCGGGAA CTGTATGAGA 851 AATATGGTGA AGATGCCTAT ACGCAGGGTT TTAAGGTTTA TACCACGGTC 901 CGCACCGATC ATCAGAAGGC GGCAACCGAG GCATTGCGCA AGGCTCTACG 951 GAATTTCGAT CGCGGCAGCA GCTACCGCGG TGCGGAAAAC TATATCGATT 1001 TGAGTAAGAG TGAAGATGTC GAGGAGACTG TCAGCCAGTA TCTGTCGGGA 1051 CTCTATACCG TCGATAAAAT GGTTCCCGCC GTTGTGTTGG ATGTTACTAA 1101 AAAGAAAAAT GTCGTCATAC AGCTGCCCGG CGGCAGGCGG GTTGCGCTTG 1151 ACAGGCGCGC CTTGGGTTTT GCGGCCCGAG CGGTCGATAA TGAGAAAATG 1201 GGGGAGGACC GTATCCGCAG GGGCGCGGTC ATCCGTGTCA AAAACAACGG 1251 CGGGCGTTGG GCGGTGGTTC AAGAGCCGTT GCTGCAGGGG GCTTTGGTTT 1301 CGCTGGATGC AAAAACCGGA GCTGTGCGCG CGCTGGTCGG CGGTTATGAT 1351 TTTCACAGCA AAACATTCAA TCGTGCCGTT CAGGCAATGC GGCAGCCGGG 1401 TTCGACCTTT AAGCCGTTTG TCTATTCGGC GGCATTATCT AAGGGGATGA 1451 CCGCGTCCAC AGTGGTTAAC GATGCGCCGA TTTCCCTGCC GGGGAAAGGG 1501 CCGAACGGTT CGGTTTGGAC ACCTAAAAAT TCAGACGGCA GATATTCCGG 1551 CTACATTACT TTGAGACAGG CTCTGACGGC TTCCAAGAAT ATGGTTTCCA 1601 TCCGTATTTT GATGTCTATC GGTGTCGGTT ACGCGCAACA GTATATCCGG 1651 CGTTTCGGCT TCAGGCCGTC CGAGCTGCCG GCAAGCCTGT CTATGGCTTT 1701 AGGTACGGGC GAGACGACGC CGTTGAAAGT GGCGGAGGCA TATAGTGTAT 1751 TTGCGAACGG CGGATATAGG GTTTCTTCGC ACGTGATCGA TAAGATTTAT 1801 GACAGAGACG GCAGGTTGCG CGCCCAAATG CAACCTTTGG TGGCAGGGCA 1851 AAATGCGCCT CAGGCAATCG ATCCGCGCAA TGCCTATATT ATGTATAAGA 1901 TTATGCAGGA TGTGGTCCGT GTCGGTACGG CAAGGGGGGC AGCTGCGTTG 1951 GGAAGAACGG ATATTGCCGG TAAAACGGGT ACGACCAACG ACAATAAAGA 2001 TGCGTGGTTT GTCGGTTTTA ACCCTGATGT GGTTACTGCC GTATATATCG 2051 GCTTCGACAA ACCTAAGAGT ATGGGGCGTG CCGGCTACGG CGGTACGATT 2101 GCGGTGCCGG TTTGGGTGGA CTATATGCGT TTTGCGTTGA AAGGAAAGCA 2151 GGGCAAAGGG ATGAAAATGC CTGAAGGTGT GGTCAGCAGC AATGGCGAAT 2201 ACTATATGAA GGAACGTATG GTAACCGATC CGGGCTTGAT GCTGGACAAC 2251 AGCGGTATTG CGCCGCAACC TTCCCGACGG GCAAAAGAAG ATGATGAAGC 2301 GGCAGTAGAA AACGAACAGC AGGGAAGGTC TGACGAAACG CGTCAGGACG 2351 TACAGGAAAC GCCGGTGCTT CCGAGCAATA CGGATTCCAA ACAGCAGCAG 2401 TTGGATTCCC TGTTTTAA This corresponds to the amino acid sequence <SEQ ID 2676; ORF 791.ng>: g791.pep 1 MVNYYSAMIK KILTTCFGLF FGFCVFGVGL VAIAILVTYP KLPSLDSLQH 51 YQPKMPLTIY SADGEVIGMY GEQRREFTKI GDFPEVLRNA VIAAEDKRFY 101 RHWGVDVWGV ARAAVGNWS GSVQSGASTI TQQVAKNFYL ΞSEKTFTRKF 151 NEVLLAYKIE QSLSKDKILE LYFNQIYLGQ RAYGFASAAQ IYFNKNVRDL 201 TLAEAAMLAG LPKAPSAYNP IVNPERAKLR QKYILNNMLE EKMITVQQRD 251 QALNEELHYE RFVRKIDQSA LYVAEMVRRE LYEKYGEDAY TQGFKVYTTV 301 RTDHQKAATE ALRKALRNFD RGSSYRGAEN YIDLΞKSEDV EETVSQYLSG 351 LYTVDKMVPA WLDVTKKKN WIQLPGGRR VALDRRALGF AARAVDNEKM 401 GEDRIRRGAV IRVKNNGGRW AVVQEPLLQG ALVSLDAKTG AVRALVGGYD 451 FHSKTFNRAV QAMRQPGSTF KPFVYSAALΞ KGMTASTWN DAPISLPGKG 501 PNGSVWTPKN SDGRYΞGYIT LRQALTASKN MVSIRILMSI GVGYAQQYIR 551 RFGFRPSELP ASLΞMALGTG ETTPLKVAEA YSVFANGGYR VSSHVIDKIY 601 DRDGRLRAQM QPLVAGQNAP QAIDPRNAYI MYKIMQDVVR VGTARGAAAL 651 GRTDIAGKTG TTNDNKDAWF VGFNPDVVTA VYIGFDKPKS MGRAGYGGTI 701 AVPVWVDYMR FALKGKQGKG MKMPEGVVSΞ NGEYYMKERM VTDPGLMLDN 751 SGIAPQPSRR AKEDDEAAVE NEQQGRSDET RQDVQETPVL PΞNTDSKQQQ 801 LDΞLF* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2677>: m791. seq 1 ATGGTAAATT ATTATTCAGC TATGATTAAA AAGATTTTAA CGACTTGTTT 51 TGGTTTGGTT TTTGGGTTTT GTGTATTTGG AGTGGGTTTG GTTGCCATTG 101 CTATTTTGGT AACGTATCCG AAACTGCCGT CTTTGGATTC TTTGCAGCAT 151 TACCAGCCTA AAATGCCGTT GACTATTTAT TCGGCGGATG GGGAAGTCAT 201 CGGTATGTAT GGGGAGCAGC GGCGCGAATT TACAAAAATC GGCGATTTCC 251 CAGAGGTGTT GCGGAATGCG GTTATCGCCG CCGAGGATAA ACGCTTTTAC 301 CGGCATTGGG GGGTGGATGT TTGGGGTGTT GCCCGCGCTG CCGTCGGCAA 351 TGTCGTGTCC GGCAGCGTGC AGTCGGGTGC GAGTACGATT ACGCAGCAGG 401 TGGCGAAAAA TTTTTATTTG AGCAGTGAAA AAACGTTCAC ACGCAAATTC 451 AATGAGGTGT TGCTTGCCTA TAAAATCGAG CAGTCTTTAA GCAAAGACAA 501 AATCCTCGAG TTGTATTTCA ATCAGATTTA CCTCGGTCAG CGCGCCTATG 551 GTTTTGCATC TGCCGCGCAA ATCTATTTCA ATAAGAATGT CCGAGATTTG 601 ACTTTGGCGG AAGCCGCCAT GCTTGCGGGA CTGCCCAAGG CTCCGTCTGC 651 CTATAATCCG ATTGTTAATC CAGAACGTGC CAAGTTGCGC CAGAAGTATA 701 TTTTGAACAA TATGCTCGAG GAGAAGATGA TTACCGTGCA ACAGCGCGAT 751 CAGGCGTTGA ATGAGGAACT GCATTACGAG CGGTTTGTTC GGAAAATCGA 801 TCAGAGTGCG TTATATGTGG CGGAAATGGT GCGTCAGGAA CTGTATGAGA 851 AATACGGTGA AGATGCCTAT ACGCAGGGTT TTAAGGTTTA TACCACGGTC 901 CGCGCCGATC ATCAGAAGGT GGCAACCGAG GCATTGCGCA AGGCTCTACG 951 GAATTTCGAT CGCGGCAGCA GCTACCGCGG TGCGGAAAAC TATATCGATT 1001 TGAGTAAGAG TGAAGATGTC GAGGAGACTG TCAGCCAGTA TCTGTCGGGA 1051 CTCTATACCG TCGATAAAAT GGTTCCCGCC GTTGTGTTGG ATGTGACTAA 1101 AAAGAAAAAT GTCGTCATAC AGCTGCCCGG CGGCAGGCGG GTTACGCTTG 1151 ACAGGCGCGC CTTGGGTTTT GCGGCCCGCG CGGTCAATAA TGAAAAAATG 1201 GGGGAGGACC GTATCCGCAG GGGCGCGGTC ATCCGTGTCA AAAACAACGG 1251 CGGGCGTTGG GCGGTGGTTC AAGAGCCGTT GCTGCAGGGG GCTTTGGGTT 1301 CGCTGGATGC AAAAACCGGA GCTGTGCGCG CGCTGGTCGG CGGTTATGAT 1351 TTTCACAGCA AAACATTCAA TCGTGCCGTT CAGGCAATGC GGCAGCCGGG 1401 TTCGACCTTT AAGCCGTTTG TCTATTCGGC GGCATTATCT AAGGGGATGA 1451 CCGCGTCCAC AGTGGTTAAC GATGCGCCGA TTTCCCTGCC GGGGAAAGGG 1501 CCGAACGGTT CGGTTTGGAC ACCTAAAAAT TCAGACGGCA GATATTCCGG 1551 CTACATTACT TTGAGACAGG CTCTGACGGC TTCCAAGAAT ATGGTTTCCA 1601 TCCGTATTTT GATGTCTATC GGTGTCGGTT ACGCGCAACA GTATATCCGG 1651 CGTTTCGGCT TCAGGTCGTC CGAGCTGCCG GCAAGCCTGT CTATGGCTTT 1701 AGGTACGGGC GAGACAACGC CGTTGAAAGT GGCGGAGGCA TATAGCGTAT 1751 TTGCGAACGG CGGATATAGG GTTTCTTCGC ACGTAATCGA TAAGATTTAT 1801 GACAGAGACG GCAGGTTGCG CGCCCAAATG CAACCTTTGG TGGCTGGGCA 1851 AAATGCGCCT CAGGCAATCG ATCCGCGCAA TGCCTATATT ATGTATAAGA 1901 TTATGCAGGA TGTGGTCCGT GTTGGTACGG CAAGGGGGGC AGCTGCGTTG 1951 GGAAGAACGG ATATTGCCGG TAAAACGGGT ACGACCAATG ACAATAAGGA 2001 TGCGTGGTTT GTCGGTTTTA ACCCTGATGT GGTTACTGCC GTATATATCG 2051 GCTTCGACAA ACCTAAGAGT ATGGGGCGTG TCGGCTACGG CGGTACGATT 2101 GCGGTGCCGG TTTGGGTGGA CTATATGCGT TTTGCGTTGA AAGGAAAGCA 2151 GGGCAAGGGG ATGAAAATGC CTGAAGGTGT GGTCAGCAGC AATGGCGAAT 2201 ACTATATGAA GGAACGTATG GTAACCGATC CGGGCTTGAC GCTGGACAAC 2251 AGCGGTATTG CGCCGCAACC TTCCCGACGG GCAAAAGAAG ATGACGGGGG 2301 CGCGGCAGAA GGCGGACGGC AGGCGGCGGA TGACGAAGTC CGCCAAGATA 2351 TGCAGGAAAC GCCGGTGCTT CCGAGTAATA CTGGTTCCAA ACAGCAGCAG 2401 TTGGATTCTC TGTTTTAA This corresponds to the amino acid sequence <SEQ ID 2678; OI [>: m791.pep 1 MVNYYSAMIK KILTTCFGLV FGFCVFGVGL VAIAILVTYP KLPSLDSLQH 51 YQPKMPLTIY SADGEVIGMY GEQRREFTKI GDFPEVLRNA VIAAEDKRFY 101 RHWGVDVWGV ARAAVGNWS GSVQSGASTI TQQVAKNFYL SSEKTFTRKF 151 NEVLLAYKIE QSLSKDKILE LYFNQIYLGQ RAYGFASAAQ IYFNKNVRDL 201 TLAEAAMLAG LPKAPSAYNP IVNPERAKLR QKYILNNMLE EKMITVQQRD 251 QALNEELHYE RFVRKIDQSA LYVAEMVRQE LYEKYGEDAY TQGFKVYTTV 301 RADHQKVATE ALRKALRNFD RGSSYRGAEN YIDLSKSEDV EETVSQYLSG 351 LYTVDKMVPA WLDVTKKKN WIQLPGGRR VTLDRRALGF AARAVNNEKM 401 GEDRIRRGAV IRVKNNGGRW AVVQEPLLQG ALGΞLDAKTG AVRALVGGYD 451 FHSKTFNRAV QAMRQPGSTF KPFVYSAALS KGMTASTWN DAPISLPGKG 501 PNGSVWTPKN ΞDGRYSGYIT LRQALTASKN MVSIRILMSI GVGYAQQYIR 551 RFGFRSSELP ASLSMALGTG ETTPLKVAEA YSVFANGGYR VSSHVIDKIY 601 DRDGRLRAQM QPLVAGQNAP QAIDPRNAYI MYKIMQDVVR VGTARGAAAL 651 GRTDIAGKTG TTNDNKDAWF VGFNPDVVTA VYIGFDKPKS MGRVGYGGTI 701 AVPVWVDYMR FALKGKQGKG MKMPEGVVΞS NGEYYMKERM VTDPGLTLDN 751 SGIAPQPSRR AKEDDGGAAE GGRQAADDEV RQDMQETPVL PΞNTGΞKQQQ 801 LDSLF* g791/ 791 97.3% identity in 805 aa overlap 10 20 30 40 50 60 g791.pep MVNYYSAMIKKILTTCFGLFFGFCVFGVGLVAIAILVTYPI (LPSLDSLQHYζ [PLTIY I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m791 MVNYYSAMIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHYQPKMPLTIY 10 20 30 40 50 60 70 80 90 100 110 120 g791.pep SADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWGVARAAVGNWS I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m791 SADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWGVARAAVGNVVS 70 80 90 100 110 120 130 140 150 160 170 180 g791.pep GSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDKILELYFNQIYLGQ I II I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m791 GSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDKILELYFNQIYLGQ 130 140 150 160 170 180 190 200 210 220 230 240 g791.pep RAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPERAKLRQKYILNNMLE I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m791 RAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPERAKLRQKYILNNMLE 190 200 210 220 230 240 250 260 270 280 290 300 g791. pep EKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRRELYEKYGEDAYTQGFKVYTTV I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M I I I I I I I I I I I I I I I I I I I I I m791 EKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYEKYGEDAYTQGFKVYTTV 250 260 270 280 290 300 310 320 330 340 350 360 g791.pep RTDHQKAATEALRKALRNFDRGSSYRGAENYIDLΞKSEDVEETVSQYLSGLYTVDKMVPA M II I M I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m791 RADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVEETVSQYLSGLYTVDKMVPA 310 320 330 340 350 360 370 380 390 400 410 420 g791 . pep VVLDVTKKKNWIQLPGGRRVALDRRALGFAARAVDNEKMGEDRIRRGAVIRVKNNGGRW I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I M l I I I I I I I I I I I I I I I I I I I I I I I m791 WLDVTKKKNWIQLPGGRRVTLDRRALGFAARAVNNEKMGEDRIRRGAVIRVKNNGGRW 370 380 390 400 410 420 430 440 450 460 470 480 g791 . pep AVVQEPLLQGALVSLDAKTGAVRALVGGYDFHΞKTFNRAVQAMRQPGSTFKPFVYSAALS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m791 AWQEPLLQGALGSLDAKTGAVRALVGGYDFHSKTFNRAVQAMRQPGSTFKPFVYSAALS 430 440 450 460 470 480 490 500 510 520 530 540 g791.pep KGMTASTWNDAPIΞLPGKGPNGSVWTPKNΞDGRYSGYITLRQALTASKNMVΞIRILMSI I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I m791 KGMTAΞTVVNDAPISLPGKGPNGSVWTPKNSDGRYSGYITLRQALTASKNMVSIRILMSI 490 500 510 520 530 540 550 560 570 580 590 600 g791.pep GVGYAQQYIRRFGFRPΞELPAΞLSMALGTGETTPLKVAEAYSVFANGGYRVSSHVIDKIY I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m791 GVGYAQQYIRRFGFRSΞELPASLSMALGTGETTPLKVAEAYSVFANGGYRVSSHVIDKIY 550 560 570 580 590 600 610 620 630 640 650 660 g791.pep DRDGRLRAQMQPLVAGQNAPQAIDPRNAYIMYKIMQDWRVGTARGAAALGRTDIAGKTG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m791 DRDGRLRAQMQPLVAGQNAPQAIDPRNAYIMYKIMQDWRVGTARGAAALGRTDIAGKTG 610 620 630 640 650 660 670 680 690 700 710 720 g791.pep TTNDNKDAWFVGFNPDWTAVYIGFDKPKSMGRAGYGGTIAVPVWVDYMRFALKGKQGKG I I II I II I I I I II I I I I I I I I I I I I I I I I I I I Ml I I I I I I I I I I I I I I I I I I I I I I I I I m791 TTNDNKDAWFVGFNPDWTAVYIGFDKPKSMGRVGYGGTIAVPVWVDYMRFALKGKQGKG 670 680 690 700 710 720 730 740 750 760 770 780 g791.pep MKMPEGWSSNGEYYMKERMVTDPGLMLDNSGIAPQPSRRAKEDDEAAVENEQQGRΞDET I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : \ : \ : : I : : I I : m791 MKMPEGWSSNGEYYMKERMVTDPGLTLDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEV 730 740 750 760 770 780 790 800 g791.pep RQDVQETPVLPSNTDSKQQQLDSLFX I I Ml I I I I I I II I I I I I I I I I I I I m791 RQDMQETPVLPΞNTGΞKQQQLDΞLFX 790 800 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2679>: a791. seq 1 ATGGTAAATT ATTATTCAGC TATGATTAAA AAGATTTTAA CGACTTGTTT 51 TGGTTTGGTT TTTGGGTTTT GTGTATTTGG AGTGGGTTTG GTTGCCATTG 101 CTATTTTGGT AACGTATCCG AAACTGCCGT CTTTGGATTC TTTGCAGCAT 151 TACCAGCCTA AAATGCCGTT GACTATTTAT TCGGCGGATG GGGAAGTCAT 201 CGGTATGTAT GGGGAGCAGC GGCGCGAATT TACAAAAATC GGCGATTTCC 251 CAGAGGTGTT GCGGAATGCG GTTATCGCCG CCGAGGATAA ACGCTTTTAC 301 CGGCATTGGG GGGTGGATGT TTGGGGTGTT GCCCGCGCTG CCGTCGGCAA 351 TGTCGTGTCC GGCAGCGTGC AGTCGGGTGC GAGTACGATT ACGCAGCAGG 401 TGGCGAAAAA TTTTTATTTG AGCAGTGAAA AAACGTTCAC ACGCAAATTC 451 AATGAGGTGT TGCTTGCCTA TAAAATCGAG CAGTCTTTAA GCAAAGACAA 501 AATCCTCGAG TTGTATTTCA ATCAGATTTA CCTCGGTCAG CGCGCCTATG 551 GTTTTGCATC TGCCGCGCAA ATCTATTTCA ATAAGAATGT CCGAGATTTG 601 ACTTTGGCGG AAGCCGCCAT GCTTGCGGGA CTGCCCAAGG CTCCGTCTGC 651 CTATAATCCG ATTGTTAATC CAGAACGTGC CAAGTTGCGC CAGAAGTATA 701 TTTTGAACAA TATGCTCGAG GAGAAGATGA TTACCGTGCA ACAGCGCGAT 751 CAGGCGTTGA ATGAGGAACT GCATTACGAG CGGTTTGTTC GGAAAATCGA 801 TCAGAGTGCT TTATATGTGG CGGAAATGGT GCGTCAGGAA CTGTATGAGA 851 AATACGGTGA AGATGCCTAT ACGCAGGGTT TTAAGGTTTA TACCACGGTC 901 CGCGCCGATC ATCAGAAGGT GGCAACCGAG GCATTGCGCA AGGCTCTACG 951 GAATTTCGAT CGCGGCAGCA GCTACCGCGG TGCGGAAAAC TATATCGATT 1001 TGAGTAAGAG TGAAGATGTC GAGGAGACTG TCAGCCAGTA TCTGTCGGGA 1051 CTCTATACCG TCGATAAAAT GGTTCCCGCC GTTGTGTTGG ATGTGACTAA 1101 AAAGAAAAAT GTCGTCATAC AGCTGCCCGG CGGCAGGCGG GTTACGCTTG 1151 ACAGGCGCGC CTTGGGTTTT GCGGCCCGCG CGGTCAATAA TGAAAAAATG 1201 GGGGAGGACC GTATCCGCAG GGGCGCGGTC ATCCGTGTCA AAAACAACGG 1251 CGGGCGTTGG GCGGTGGTTC AAGAGCCGTT GCTGCAGGGG GCTTTGGTTT 1301 CGCTGGATGC AAAAACCGGA GCTGTGCGCG CGCTGGTCGG CGGTTATGAT 1351 TTTCACAGCA AAACATTCAA TCGTGCCGTT CAGGCAATGC GGCAGCCGGG 1401 TTCGACCTTT AAGCCGTTTG TCTATTCGGC GGCATTATCT AAGGGGATGA 1451 CCGCGTCCAC AGTGGTTAAC GATGCGCCGA TTTCCCTGCC GGGGAAAGGG 1501 CCGAACGGTT CGGTTTGGAC ACCTAAAAAT TCAGACGGCA GATATTCCGG 1551 CTACATTACT TTGAGACAGG CTCTGACGGC TTCCAAGAAT ATGGTTTCCA 1601 TCCGTATTTT GATGTCTATC GGTGTCGGTT ACGCGCAACA GTATATCCGG 1651 CGTTTCGGCT TCAGGTCGTC CGAGCTGCCG GCAAGCCTGT CTATGGCTTT 1701 AGGTACGGGC GAGACAACGC CGTTGAAAGT GGCGGAGGCA TATAGCGTAT 1751 TTGCGAACGG CGGATATAGG GTTTCTTCGC ACGTAATCGA TAAGATTTAT 1801 GACAGAGACG GCAGGTTGCG CGCCCAAATG CAACCTTTGG TGGCCGGGCA 1851 AAATGCGCCT CAGGCAATCG ATCCGCGCAA TGCCTATATT ATGTATAAGA 1901 TTATGCAGGA TGTGGTCCGT GTTGGTACGG CAAGGGGGGC AGCTGCGTTG 1951 GGAAGAACGG ATATTGCCGG TAAAACGGGT ACGACCAATG ACAATAAGGA 2001 TGCGTGGTTT GTCGGTTTTA ACCCTGATGT GGTTACTGCC GTATATATCG 2051 GCTTCGACAA ACCTAAGAGT ATGGGGCGTG TCGGCTACGG CGGTACGATT 2101 GCGGTGCCGG TTTGGGTGGA CTATATGCGT TTTGCGTTGA AAGGAAAGCA 2151 GGGCAAGGGG ATGAAAATGC CTGAAGGTGT GGTCAGCAGC AATGGCGAAT 2201 ACTATATGAA GGAACGTATG GTAACCGATC CGGGCTTGAC GCTGGACAAC 2251 AGCGGTATTG CGCCGCAACC TTCCCGACGG GCAAAAGAAG ATGACGGGGG 2301 CGCGGCAGAA GGCGGACGGC AGGCGGCGGA TGACGAAGTC CGCCAAGATA 2351 TGCAGGAAAC GCCGGTGCTT CCGAGTAATA CTGGTTCCAA ACAGCAGCAG 2401 TTGGATTCTC TGTTTTAA This corresponds to the amino acid sequence <SEQ ID 2680; ORF 791.a>: a791.pep 1 MVNYYSAMIK KILTTCFGLV FGFCVFGVGL VAIAILVTYP KLPSLDSLQH 51 YQPKMPLTIY SADGEVIGMY GEQRREFTKI GDFPEVLRNA VIAAEDKRFY 101 RHWGVDVWGV ARAAVGNWS GSVQSGASTI TQQVAKNFYL SSEKTFTRKF 151 NEVLLAYKIE QSLSKDKILE LYFNQIYLGQ RAYGFASAAQ IYFNKNVRDL 201 TLAEAAMLAG LPKAPSAYNP IVNPERAKLR QKYILNNMLE EKMITVQQRD 251 QALNEELHYE RFVRKIDQSA LYVAEMVRQE LYEKYGEDAY TQGFKVYTTV 301 RADHQKVATE ALRKALRNFD RGSSYRGAEN YIDLSKΞEDV EETVSQYLSG 351 LYTVDKMVPA WLDVTKKKN WIQLPGGRR VTLDRRALGF AARAVNNEKM 401 GEDRIRRGAV IRVKNNGGRW AVVQEPLLQG ALVSLDAKTG AVRALVGGYD 451 FHSKTFNRAV QAMRQPGSTF KPFVYSAALS KGMTASTWN DAPISLPGKG 501 PNGSVWTPKN SDGRYSGYIT LRQALTASKN MVSIRILMSI GVGYAQQYIR 551 RFGFRSSELP ASLSMALGTG ETTPLKVAEA YSVFANGGYR VSSHVIDKIY 601 DRDGRLRAQM QPLVAGQNAP QAIDPRNAYI MYKIMQDVVR VGTARGAAAL 651 GRTDIAGKTG TTNDNKDAWF VGFNPDVVTA VYIGFDKPKS MGRVGYGGTI 701 AVPVWVDYMR FALKGKQGKG MKMPEGWSS NGEYYMKERM VTDPGLTLDN 751 SGIAPQPSRR AKEDDGGAAE GGRQAADDEV RQDMQETPVL PSNTGSKQQQ 801 LDΞLF* a791/m791 99.9% identity in 805 aa overlap 10 20 30 40 50 60 a791.pep MVNYYSAMIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHYQPKMPLTIY I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m791 MVNYYSAMIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHYQPKMPLTIY 10 20 30 40 50 60 70 80 90 100 110 120 a791 ■pep SADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWGVARAAVGNWS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I πι791 SADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWGVARAAVGNWS 70 80 90 100 110 120 130 140 150 160 170 180 a791. ■pep GSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDKILELYFNQIYLGQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m791 GSVQSGAΞTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLΞKDKILELYFNQIYLGQ 130 140 150 160 170 180 190 200 210 220 230 240 a791, ■pep RAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPERAKLRQKYILNNMLE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m791 RAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPERAKLRQKYILNNMLE 190 200 210 220 230 240 250 260 270 280 290 300 a791. ■pep EKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYEKYGEDAYTQGFKVYTTV I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m791 EKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYEKYGEDAYTQGFKVYTTV 250 260 270 280 290 300 310 320 330 340 350 360 a791. .pep RADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVEETVSQYLSGLYTVDKMVPA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m791 RADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVEETVSQYLSGLYTVDKMVPA 310 320 330 340 350 360 370 380 390 400 410 420 a791. ■pep VVLDVTKKKNWIQLPGGRRVTLDRRALGFAARAVNNEKMGEDRIRRGAVIRVKNNGGRW I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m791 WLDVTKKKNWIQLPGGRRVTLDRRALGFAARAVNNEKMGEDRIRRGAVIRVKNNGGRW 370 380 390 400 410 420 430 440 450 460 470 480 a791.pep AVVQEPLLQGALVΞLDAKTGAVRALVGGYDFHSKTFNRAVQAMRQPGSTFKPFVYSAALS I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m791 AVVQEPLLQGALGSLDAKTGAVRALVGGYDFHSKTFNRAVQAMRQPGSTFKPFVYΞAALS 430 440 450 460 470 480 490 500 510 520 530 540 a791 . pep KGMTASTWNDAPISLPGKGPNGΞVWTPKNSDGRYΞGYITLRQALTASKNMVS IRILMSI I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m791 KGMTASTWNDAPISLPGKGPNGSVWTPKNSDGRYSGYITLRQALTASKNMVSIRILMSI 490 500 510 520 530 540 550 560 570 580 590 600 a791 . pep GVGYAQQYIRRFGFRSΞELPASLSMALGTGETTPLKVAEAYSVFANGGYRVSSHVIDKIY I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m791 GVGYAQQYIRRFGFRSSELPASLSMALGTGETTPLKVAEAYSVFANGGYRVSSHVIDKIY 550 560 570 580 590 600 610 620 630 640 650 660 a791.pep DRDGRLRAQMQPLVAGQNAPQAIDPRNAYIMYKIMQDWRVGTARGAAALGRTDIAGKTG I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m791 DRDGRLRAQMQPLVAGQNAPQAIDPRNAYIMYKIMQDWRVGTARGAAALGRTDIAGKTG 610 620 630 640 650 660 670 680 690 700 710 720 a791.pep TTNDNKDAWFVGFNPDWTAVYIGFDKPKSMGRVGYGGTIAVPVWVDYMRFALKGKQGKG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m791 TTNDNKDAWFVGFNPDVVTAVYIGFDKPKSMGRVGYGGTIAVPVWVDYMRFALKGKQGKG 670 680 690 700 710 720 730 740 750 760 770 780 a791 . pep MKMPEGWSΞNGEYYMKERMVTDPGLTLDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M I m791 MKMPEGWSSNGEYYMKERMVTDPGLTLDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEV 730 740 750 760 770 780 790 800 a791.pep RQDMQETPVLPSNTGSKQQQLDSLFX I II I I I I I I I I I I I I I I I I I I I I I I I m791 RQDMQETPVLPSNTGSKQQQLDSLFX 790 800 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2681>: g792.seq 1 ATGTTCCGCA TCGTCAAATG GCTGATTGCC CTGCCCGTCG GCATCTTTAT 51 CTTTTTCAAT GCCTATGTGT ACGGCAACAT CATCACCTAC CGCGCCGTCG 101 CGCCCCATCG GACTGCCTTT ATGTCGATGC GGATGAAGCA GTTTGAACAA 151 GAAGGTCGCG ATGTCGCACT GGATTACCGC TGGGTGCCCT ACAACCGCAT 201 TTCCACCAAC CTGAAAAAAG CCCTGATTGC TTCCGAAGAT GTCCGTTTTG 251 CCggacacgg gggcttcGat GGGGACGGCa tTCAAAACGC CATCAGGCGC 301 AACCGGAACA GCGGCGAAGT GAAGGCGGGC GGATCGACCA TCAGCCAGCA 351 GCTTGCCAAA AACCTCTTCC TCAACGAAAG CCGCAACTAT CTGCGCAAAG 401 GGGAAGAGGC GGCCATTACG GCAATGATGG AAGCTGTTAC CGACAAAAAC 451 AGGATTTTCG AACTGTATTT AAACTCAATC GAATGGCACT ACGGCgtTTT 501 CGGCGCGGAA GCTGCGTCCC GGtatTttTA TAAAAAACCG GCcgcaGACC 551 TGACcAAACA GCAggcggcG aaactgacgg tactcgtccc cgccccgttt 601 tactactctg accatccaaa aagcaaacgg ctgcgcaaca aaaccaatat 651 cgtgctcaga cgcatgggtt cggcaaatta ccccaaagcg aaacggactg 701 attgttccag atatggaaat gccgcctgaa ctggggttcg aacggcatat 751 gttttctggg acttataa This corresponds to the amino acid sequence <SEQ ID 2682; ORF 792.ng>: g792.pep 1 MFRIVKWLIA LPVGIFIFFΝ AYVYGΝIITY RAVAPHRTAF MSMRMKQFEQ 51 EGRDVALDYR WVPYΝRISTΝ LKKALIASED VRFAGHGGFD GDGIQΝAIRR 101 ΝRΝΞGEVKAG GΞTISQQLAK ΝLFLΝEΞRΝY LRKGEEAAIT AMMEAVTDKΝ 151 RIFELYLΝSI EWHYGVFGAE AASRYFYKKP AADLTKQQAA KLTVLVPAPF 201 YYSDHPKSKR LRΝKTΝIVLR RMGSAΝYPKA KRTDCSRYGΝ AA*TGVRTAY 251 VFWDL* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2683>: 792.seq 1 ATGTTCCGCA TCATCAAATG GCTGATTGCC CTGCCCGTCG GCATCTTTAT 51 CTTTTTCAAT GCCTATGTGT ACGGCAACAT CATTACCTAC CGCGCCGTCG 101 CGCCCCATCG GACTGCCTTT ATGTCGATGC GGATGAAGCA GTTTGAACAG 151 GAAGGTCGCG ATGTCGCACT GGATTACCGC TGGATGCCCT ACAAACGCAT 201 TTCCACCAAC CTGAAAAAAG CCCTGATTGC TTCCGAAGAT GCCCGTTTCG 251 CCGGGCACGG CGGCTTCGAT TGGGGCGGCA TTCAAAACGC CATCAGGCGC 301 AACCGGAACA GCGGCAAAGT GAAGGCGGGC GGCTCGACCA TCAGCCAGCA 351 GCTTGCCAAA AACCTGTTTT TAAACGAAAG CCGCAGCTAT ATCCGCAAAG 401 GCGAAGAAGC GGCGATTACC GCGATGATGG AAGCCGTTAC CGACAAAGAC 451 AGGATTTTTG AACTGTATTT AAACTCAATC GAATGGCACT ACGGCGTTTT 501 CGGCGCGGAA GCCGCGTCCC GGTATTTTTA TCAAATACCC GCCGCCAAGC 551 TGACCAAACA GCAGGCGGCA AAACTGACGG CGCGCGTCCC CGCCCCGCTC 601 TACTACGCCG ACCATCCGAA AAGCAAACGG CTCCGCAACA AAACCAATAT 651 CGTGCTCAAA CGCATGGGTT CGGCAGAGTT GCCTGAAAGC GACACGGACT 701 GA This corresponds to the amino acid sequence <SEQ ID 2684; ORF 792>: m792.pep 1 MFRIIKWLIA LPVGIFIFFΝ AYVYGΝIITY RAVAPHRTAF MSMRMKQFEQ 51 EGRDVALDYR WMPYKRISTΝ LKKALIASED ARFAGHGGFD WGGIQΝAIRR 101 ΝRΝSGKVKAG GSTISQQLAK ΝLFLΝESRSY IRKGEEAAIT AMMEAVTDKD 151 RIFELYLΝSI EWHYGVFGAE AASRYFYQIP AAKLTKQQAA KLTARVPAPL 201 YYADHPKSKR LRΝKTΝIVLK RMGSAELPEΞ DTD* g792 / 792 90.4% identity in 230 aa overlap 10 20 30 40 50 60 g792.pep MFRIVKWLIALPVGIFIFFΝAYVYGΝIITYRAVAPHRTAFMSMRMKQFEQEGRDVALDYR I II Ml I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M I I M m792 MFRIIKWLIALPVGIFIFFΝAYVYGΝIITYRAVAPHRTAFMSMRMKQFEQEGRDVALDYR 10 20 3 0 40 50 60 70 80 90 100 110 120 g792.pep WVPYΝRISTΝLKKALIASEDVRFAGHGGFDGDGIQΝAIRRΝRΝSGEVKAGGSTISQQLAK I : I I : I I I I I I I I I I I I I I Ml I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I m792 WMPYKRISTNLKKALIASEDARFAGHGGFDWGGIQNAIRRNRNSGKVKAGGSTISQQLAK 70 80 90 100 110 120 130 140 150 160 170 180 g792.pep NLFLNEΞRNYLRKGEEAAITAMMEAVTDKNRIFELYLNSIEWHYGVFGAEAASRYFYKKP I I I I I I I I : I : I I I I I I I I I I I I I I I I I Ml I I I I I I I I I I I I I I I I I I I I I I I I I I : I m792 NLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSIEWHYGVFGAEAASRYFYQIP 130 140 150 160 170 180 190 200 210 220 230 240 g792.pep AADLTKQQAAKLTVLVPAPFYYSDHPKSKRLRNKTNIVLRRMGΞANYPKAKRTDCSRYGN II I I I I I II I I I : I I I M I I : I I I I I I I I I I I I I I I I : I I I I I : M: m792 AAKLTKQQAAKLTARVPAPLYYADHPKSKRLRNKTNIVLKRMGSAELPESDTDX 190 200 210 220 230 250 g792.pep AAXTGVRTAYVFWDLX The following partial DNA sequence was identified in N meningitidis <SEQ ID 2685>: a792.seq 1 ATGTTCCGCA TCATCAAATG GCTGATTGCC CTGCCCGTCG GCATCTTTAT 51 CTTTTTCAAT GCCTATGTGT ACGGCAACAT CATTACCTAC CGCGCCGTCG 101 CGCCCCATCG GACTGCCTTT ATGTCGATGC GGATGAAGCA GTTTGAACAG 151 GAAGGTCGCG ATGTCGCACT GGATTACCGC TGGATGCCCT ACAAACGCAT 201 TTCCACCAAC CTGAAAAAAG CCCTGATTGC TTCCGAAGAT GCCCGTTTCG 251 CCGGGCACGG CGGCTTCGAT TGGGGCGGCA TTCAAAACGC CATCAGGCGC 301 AACCGGAACA GCGGCAAAGT GAAGGCGGGC GGCTCGACCA TCAGCCAGCA 351 GCTTGCCAAA AACCTGTTTT TAAACGAAAG CCGCAGCTAT ATCCGCAAAG 401 GCGAAGAAGC GGCGATTACC GCGATGATGG AAGCCGTTAC CGACAAAGAC 451 AGGATTTTTG AACTGTATTT AAACTCAATC GAATGGCACT ACGGCGTTTT 501 CGGCGCGGAA GCCGCGTCCC GGTATTTTTA TCAAATACCC GCCGCCAAGC 551 TGACCAAACA GCAGGCGGCA AAACTGACGG CGCGCGTCCC CGCCCCGCTC 601 TACTACGCCG ACCATCCGAA AAGCAAACGG CTCCGCAACA AAACCAATAT 651 CGTGCTCAGA CGCATGGGTT CGGCAGAGTT GCCTGAAAGC GACACGGACT 701 GA This corresponds to the amino acid sequence <SEQ ID 2686; ORF 792. a>: a792.pep 1 MFRIIKWLIA LPVGIFIFFΝ AYVYGΝIITY RAVAPHRTAF MSMRMKQFEQ 51 EGRDVALDYR WMPYKRISTΝ LKKALIASED ARFAGHGGFD WGGIQΝAIRR 101 ΝRΝΞGKVKAG GSTISQQLAK ΝLFLΝESRSY IRKGEEAAIT AMMEAVTDKD 151 RIFELYLΝSI EWHYGVFGAE AASRYFYQIP AAKLTKQQAA KLTARVPAPL 201 YYADHPKΞKR LRΝKTΝIVLR RMGΞAELPES DTD* m792/a792 99.6% identity in 233 aa overlap 10 20 30 40 50 60 a792.pep MFRIIKWLIALPVGIFIFFΝAYVYGΝIITYRAVAPHRTAFMSMRMKQFEQEGRDVALDYR I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m 92 MFRIIKWLIALPVGIFIFFΝAYVYGΝIITYRAVAPHRTAFMSMRMKQFEQEGRDVALDYR 10 20 30 40 50 60 70 80 90 100 110 120 a792.pep WMPYKRISTΝLKKALIAΞEDARFAGHGGFDWGGIQΝAIRRΝRΝSGKVKAGGSTISQQLAK I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m792 WMPYKRISTΝLKKALIASEDARFAGHGGFDWGGIQΝAIRRΝRΝSGKVKAGGSTIΞQQLAK 70 80 90 100 110 120 130 140 150 160 170 180 a792.pep ΝLFLΝESRSYIRKGEEAAITAMMEAVTDKDRIFELYLΝSIEWHYGVFGAEAASRYFYQIP I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m792 ΝLFLΝESRSYIRKGEEAAITAMMEAVTDKDRIFELYLΝSIEWHYGVFGAEAASRYFYQIP 130 140 150 160 170 180 190 200 210 220 230 a792.pep AAKLTKQQAAKLTARVPAPLYYADHPKSKRLRΝKTΝIVLRRMGSAELPEΞDTDX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I m792 AAKLTKQQAAKLTARVPAPLYYADHPKSKRLRΝKTΝIVLKRMGΞAELPESDTDX 190 200 210 220 230 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 2687>: g793. seq 1 ATGTTGATTA AAAGCGAATA TAAGCCCCGG ATGCTGCCCA AAGAAGAGCA 51 GGTCAAAAAG CCGATGACCA GTAACGGACG GATTAGCTTC GTCCTGATGG 101 CAATGGCGGT CTTGTTTGCC TGTCTGATTG CCCGCGGGCT GTATCTGCAG 151 ACGGTAACGT ATAACTTTTT GAAAGAACAG GGCGACAACC GGATTGTGCG 201 GACTCAAGCA TTGCCGGCTA CACGCGGTAC GGTTTCGGAC CGGAACGGTG 251 CGGTTTTGGC GTTGAGCGCG CCGACGGAGT CCCTGTTTGC CGTGCCTAAA 301 GATATGAAGG AAATGCCGTC TGCCGCCCAA TTGGAACGCC TGTCCGAGCT 351 TGTCGATGTG CCGGTCGATG TTTTGAGGAA CAAACTCGAA CAGAAAGGCA 401 AGTCGTTTAT TTGGATCAAG CGGCAGCTCG ATCCCAAGGT TGCCGAAGAG 451 GTCAAAGCCT TGGGTTTGGA AAACTTTGTA TTTGAAAAAG AATTAAAACG 501 CCATTACCCG ATGGGCAACC TGTTTGCACA CGTCATCGGA TTTACCGATA 551 TTGACGGCAA AGGTCAGGAA GGTTTGGAAC TTTCGCTTGA AGACAGCCTG 601 TATGGCGAAG ACGGCGCGGA AGTTGTTTTG CGGGACCGGC AGGGCAATAT 651 TGTGGACAGC TTGGACTCCC CGCGCAATAA AGCACCGCAA AACGGCAAAG 701 ACATCATCCT TTCCCTCGAT CAGAGGATTC AGACCTTGGC CTATGAAGAG 751 TTGAACAAGG CGGTCGAATA CCATCAGGCA AAAGCCGGAA CGGTGGTGGT 801 TTTGGATGCC CGCACGGGGG AAATCCTCGC CTTGGCCAAT ACGCCCGCCT 851 ACGATCCCAA CAGACCCGGC CGGGCAGACA GCGAACAGCG GCGCAACCGT 901 GCCGTAACCG ATATGATCGA ACCTGGTTCG GCAATCAAAC CGTTCGTGAT 951 TGCGAAGGCA TTGGATGCGG GCAAAACCGA TTTGAACGAA CGGCTGAATA 1001 CGCAGCCTTA TAAAATCGGA CCGTCTCCCG TGCGCGATGA TACCCATGTT 1051 TACCCCTCTT TGGATGTGCG CGGCATTATG CAGAAATCGT CCAACGTCGG 1101 CACAAGCAAA CTGTCTGCGC GTTTCGGCGC CGAAGAAATG TATGACTTCT 1151 ATCATGAATT GGGCATCGGT GTGCGTATGC ACTCGGGCTT TCCGGGGGAA 1201 ACTGCAGGTT TGTTGAGAAA TTGGCGCAGG TGGCGGCCCA TCGAACAGGC 1251 GACGATGTCT TTCGGTTACG GTCTGCAATT GAGCCTGCTG CAATTGGCGC 1301 GCGCCTATAC CGCACTGACG CACGACGGCG TTTTGCTGCC GCTCAGCTTT 1351 GAGAAGCAGG CGGTTGCGCC GCAAGGCAAA CGCATATTCA AAGAATCGAC 1401 CGCGCGCGAG GTACGCAATC TGATGGTTTC CGTAACCGAG CCGGGCGGCA 1451 CCGGTACGGC GGGTGCGGTG GACGGTTTCG ATGTCGGCGC TAAAACCGGC 1501 ACGGCGCGCA AGTTCGTCAA CGGGCGTTAT GCCGACAACA AACACGTCGC 1551 TACCTTTATC GGTTTTGCCC CCGCCAAAAA CCCCCGTGTG ATTGTGGCGG 1601 TAACCATCGA CGAACCGACT GCCCACGGCT ATTACGGCGG CGTAGTGGCA 1651 GGGCCGCCCT TCAAAAAAAT TATGGGCGGC AGCCTGAACA TCTTGGGCAT 1701 TTCCCCGACC AAGCCACTGA CCGCCGCAGC CGTCAAAACA CCGTCTTAA This corresponds to the amino acid sequence <SEQ ID 2688; ORF 793.ng>: g793.pep 1 MLIKSEYKPR MLPKEEQVKK PMTSNGRISF VLMAMAVLFA CLIARGLYLQ 51 TVTYNFLKEQ GDNRIVRTQA LPATRGTVΞD RNGAVLALΞA PTESLFAVPK 101 DMKEMPSAAQ LERLΞELVDV PVDVLRNKLE QKGKSFIWIK RQLDPKVAEE 151 VKALGLENFV FEKELKRHYP MGNLFAHVIG FTDIDGKGQE GLELΞLEDΞL 201 YGEDGAEWL RDRQGNIVDS LDSPRNKAPQ NGKDIILΞLD QRIQTLAYEE 251 LNKAVEYHQA KAGTVWLDA RTGEILALAN TPAYDPNRPG RADSEQRRNR 301 AVTDMIEPGS AIKPFVIAKA LDAGKTDLNE RLNTQPYKIG PSPVRDDTHV 351 YPSLDVRGIM QKSSNVGTΞK LSARFGAEEM YDFYHELGIG VRMHSGFPGE 401 TAGLLRNWRR WRPIEQATMS FGYGLQLΞLL QLARAYTALT HDGVLLPLSF 451 EKQAVAPQGK RIFKESTARE VRNLMVΞVTE PGGTGTAGAV DGFDVGAKTG 501 TARKFVNGRY ADNKHVATFI GFAPAKNPRV IVAVTIDEPT AHGYYGGWA 551 GPPFKKIMGG SLNILGISPT KPLTAAAVKT PS* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2689>: m793. seq 1 ATGTTGATTA AGAGCGAATA TAAGCCTCGG ATGCTGCCCA AAGAAGAGCA 51 GGTCAAAAAG CCGATGACCA GTAACGGACG GATCAGCTTC GTCCTGATGG 101 CAATAGCGGT CTTGTTTGCC GGTCTGATTG CTCGCGGACT GTATCTGCAG 151 ACGGTAACGT ATAACTTTTT GAAAGAACAG GGCGACAACC GGATTGTGCG 201 GACTCAAACA TTGCCGGCTA CACGCGGTAC GGTTTCGGAC CGGAACGGTG 251 CGGTTTTGGC GTTGAGTGCG CCGACGGAGT CCCTGTTTGC CGTGCCTAAA 301 GAGATGAAGG AAATGCCGTC TGCCGCACAA TTGGAACGCC TGTCCGAGCT 351 TGTCGATGTG CCGGTTGATG TTTTGAGGAA CAAGCTCGAA CAGAAAGGCA 401 AGTCGTTTAT CTGGATTAAG CGGCAGCTCG ATCCCAAGGT TGCCGAAGAG 451 GTCAAAGCCT TGGGTTTGGA AAACTTTGTA TTTGAAAAAG AATTAAAACG 501 CCATTACCCG ATGGGCAACC TGTTTGCACA CGTCATCGGA TTTACCGATA 551 TTGACGGCAA AGGTCAGGAA GGTTTGGAAC TTTCGCTTGA AGACAGCCTG 601 CATGGCGAAG ACGGCGCGGA AGTCGTTTTG CGGGACCGGC AGGGCAATAT 651 TGTGGACAGC TTGGACTCCC CGCGCAATAA AGCCCCGAAA AACGGCAAAG 701 ACATCATCCT TTCCCTCGAT CAGAGGATTC AGACCTTGGC CTATGAAGAG 751 TTGAACAAGG CGGTCGAATA CCATCAGGCA AAAGCCGGAA CGGTGGTGGT 801 TTTGGATGCC CGCACGGGGG AAATCCTCGC CTTGGCCAAT ACGCCCGCCT 851 ACGATCCCAA CAGGCCCGGC CGGGCAGACA GCGAACAGCG GCGCAACCGT 901 GCCGTAACCG ATATGATCGA ACCCGGTTCG GCAATCAAAC CGTTTGTGAT 951 TGCGAAGGCA TTGGATGCGG GCAAAACCGA TTTGAACGAA CGGCTGAATA 1001 CGCAGCCTTA TAAAATCGGA CCGTCTCCCG TGCGCGATAC CCATGTTTAC 1051 CCCTCTTTGG ATGTGCGCGG CATCATGCAG AAATCGTCCA ACGTCGGCAC 1101 AAGCAAACTG TCTGCGCGTT TCGGTGCCGA AGAAATGTAT GACTTCTATC 1151 ATGAGTTGGG CATCGGTGTG CGTATGCACT CGGGCTTTCC GGGCGAAACT 1201 GCAGGTTTGT TGAGAAATTG GCGCAGGTGG CGGCCTATCG AACAGGCGAC 1251 GATGTCTTTC GGTTACGGCC TGCAATTGAG CCTGCTGCAA TTGGCGCGCG 1301 CCTATACCGC ACTGACGCAC GACGGCGTTT TACTGCCGGT CAGCTTTGAA 1351 AAACAGGCGG TTGCGCCGCA AGGCAAACGC ATATTCAAAG AATCGACCGC 1401 GCGCGAGGTA CGCAATCTGA TGGTTTCCGT AACCGAGCCG GGCGGCACCG 1451 GTACGGCGGG TGCGGTGGAC GGTTTCGATG TCGGCGCGAA AACCGGCACG 1501 GCGCGCAAGT TCGTCAACGG GCGTTATGCC GACAACAAAC ACATCGCTAC 1551 CTTTATCGGT TTTGCCCCCG CCAAAAATCC CCGTGTGATT GTGGCGGTAA 1601 CCATTGACGA ACCGACTGCC CACGGTTATT ACGGCGGCGT AGTGGCAGGG 1651 CCGCCCTTCA AAAAAATTAT GGGCGGCAGC CTGAACATCT TGGGCATTTC 1701 CCCGACCAAG CCACTGACCG CCGCAGCCGT CAAAACACCG TCTTAA This corresponds to the amino acid sequence <SEQ ID 2690; OI m793.pep 1 MLIKΞEYKPR MLPKEEQVKK PMTSNGRISF VLMAIAVLFA GLIARGLYLQ 51 TVTYNFLKEQ GDNRIVRTQT LPATRGTVSD RNGAVLALSA PTESLFAVPK 101 EMKEMPSAAQ LERLΞELVDV PVDVLRNKLE QKGKSFIWIK RQLDPKVAEE 151 VKALGLENFV FEKELKRHYP MGNLFAHVIG FTDIDGKGQE GLELSLEDSL 201 HGEDGAEWL RDRQGNIVDS LDSPRNKAPK NGKDIILSLD QRIQTLAYEE 251 LNKAVEYHQA KAGTVWLDA RTGEILALAN TPAYDPNRPG RADSEQRRNR 301 AVTDMIEPGS AIKPFVIAKA LDAGKTDLNE RLNTQPYKIG PSPVRDTHVY 351 PSLDVRGIMQ KSSNVGTSKL SARFGAEEMY DFYHELGIGV RMHSGFPGET 401 AGLLRNWRRW RPIEQATMSF GYGLQLSLLQ LARAYTALTH DGVLLPVSFE 451 KQAVAPQGKR IFKESTAREV RNLMVSVTEP GGTGTAGAVD GFDVGAKTGT 501 ARKFVNGRYA DNKHIATFIG FAPAKNPRVI VAVTIDEPTA HGYYGGWAG 551 PPFKKIMGGS LNILGIΞPTK PLTAAAVKTP S* g793/ 793 98.5% identity in 582 aa overlap 10 20 3C ) 40 50 60 g793.pep MLIKSEYKPRMLPKEEQVKKPMTΞNGRISϊ ^VLMAMAVLFAC :LIARWG3LLYYLLQQTT\VTYNFLKEQ II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I m793 MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIARGLYLQTVTYNFLKEQ 10 20 30 40 50 60 70 80 90 100 110 120 g793.pep GDNRIVRTQALPATRGTVSDRNGAVLALSAPTESLFAVPKDMKEMPSAAQLERLSELVDV I I I I II I I l I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I m793 GDNRIVRTQTLPATRGTVΞDRNGAVLALSAPTESLFAVPKEMKEMPSAAQLERLSELVDV 70 80 90 100 110 120 130 140 150 160 170 180 g793.pep PVDVLRNKLEQKGKΞFIWIKRQLDPKVAEEVKALGLENFVFEKELKRHYPMGNLFAHVIG I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m793 PVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFVFEKELKRHYPMGNLFAHVIG 130 140 150 160 170 180 190 200 210 220 230 240 g793 . pep FTDIDGKGQEGLELΞLEDSLYGEDGAEWLRDRQGNIVDSLDSPRNKAPQNGKDI ILSLD I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I : | I I I I I I I I I m793 FTDIDGKGQEGLELSLEDSLHGEDGAEWLRDRQGNIVDΞLDΞPRNKAPKNGKDIILSLD 190 200 210 220 230 240 250 260 270 280 290 300 g793.pep QRIQTLAYEELNKAVEYHQAKAGTVWLDARTGEILALANTPAYDPNRPGRADΞEQRRNR I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m793 QRIQTLAYEELNKAVEYHQAKAGTVWLDARTGEILALANTPAYDPNRPGRADSEQRRNR 250 260 270 280 290 300 310 320 330 340 350 360 g793.pep AVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNTQPYKIGPSPVRDDTHVYPSLDVRGIM I I I I II I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I lllll m793 AVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNTQPYKIGPSPVRD-THVYPSLDVRGIM 310 320 330 340 350 370 380 390 400 410 420 g793.pep QKΞSNVGTSKLSARFGAEEMYDFYHELGIGVRMHSGFPGETAGLLRNWRRWRPIEQATMS I M III I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I II I I I I I I II I I I I I I I m 93 Q KSSNVGTSKLSARFGAEEMYDFYHELGIGVRMHSGFPGETAGLLRNWRRWRPIEQATMS 360 370 380 3 90 400 410 430 440 450 460 470 480 g793.pep FGYGLQLSLLQLARAYTALTHDGVLLPLSFEKQAVAPQGKRIFKESTAREVRNLMVΞVTE I I I I II I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m793 FGYGLQLSLLQLARAYTALTHDGVLLPVSFEKQAVAPQGKRIFKESTAREVRNLMVSVTE 420 430 440 450 460 470 490 500 510 520 530 540 g793.pep PGGTGTAGAVDGFDVGAKTGTARKFVNGRYADNKHVATFIGFAPAKNPRVIVAVTIDEPT I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I m793 PGGTGTAGAVDGFDVGAKTGTARKFVNGRYADNKHIATFIGFAPAKNPRVIVAVTI DEPT 480 490 500 510 520 530 550 560 570 580 g793 . pep AHGYYGGWAGPPFKKIMGGSLNILGIΞPTKPLTAAAVKTPSX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m793 AHGYYGGVVAGPPFKKIMGGSLNILGISPTKPLTAAAVKTPSX 540 550 560 570 580 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2691>: a793.seq 1 ATGTTGATTA AGAGCGAATA TAAGCCTCGG ATGCTGCCCA AAGAAGAGCA 51 GGTCAAAAAG CCGATGACCA GTAACGGACG GATCAGCTTC GTCCTGATGG 101 CAATAGCGGT CTTGTTTGCC GGTCTGATTG CTCGCGGACT GTATCTGCAG 151 ACGGTAACGT ATAACTTTTT GAAAGAACAG GGCGACAACC GGATTGTGCG 201 GACTCAAACA TTGCCGGCTA CACGCGGTAC GGTTTCGGAC CGGAACGGTG 251 CGGTTTTGGC GTTGAGTGCG CCGACGGAGT CCCTGTTTGC CGTGCCTAAA 301 GAGATGAAGG AAATGCCGTC TGCCGCACAA TTGGAACGCC TGTCCGAGCT 351 TGTCGATGTG CCGGTTGATG TTTTGAGGAA CAAGCTCGAA CAGAAAGGCA 401 AGTCGTTTAT CTGGATTAAG CGGCAGCTCG ATCCCAAGGT TGCCGAAGAG 451 GTCAAAGCCT TGGGTTTGGA AAACTTTGTA TTTGAAAAAG AATTAAAACG 501 CCATTACCCG ATGGGCAACC TGTTTGCACA CGTCATCGGA TTTACCGATA 551 TTGACGGCAA AGGTCAGGAA GGTTTGGAAC TTTCGCTTGA AGACAGCCTG 601 CATGGCGAAG ACGGCGCGGA AGTCGTTTTG CGGGACCGGC AGGGCAATAT 651 TGTGGACAGC TTGGACTCCC CGCGCAATAA AGCCCCGAAA AACGGCAAAG 701 ACATCATCCT TTCCCTCGAT CAGAGGATTC AGACCTTGGC CTATGAAGAG 751 TTGAACAAGG CGGTCGAATA CCATCAGGCA AAAGCCGGAA CGGTGGTGGT 801 TTTGGATGCC CGCACGGGGG AAATCCTCGC CTTGGCCAAT ACGCCCGCCT 851 ACGATCCCAA CAGGCCCGGC CGGGCAGACA GCGAACAGCG GCGCAACCGT 901 GCCGTAACCG ATATGATCGA ACCCGGTTCG GCAATCAAAC CGTTTGTGAT 951 TGCGAAGGCA TTGGATGCGG GCAAAACCGA TTTGAACGAA CGGCTGAATA 1001 CGCAGCCTTA TAAAATCGGA CCGTCTCCCG TGCGCGATAC CCATGTTTAC 1051 CCCTCTTTGG ATGTGCGCGG CATCATGCAG AAATCGTCCA ACGTCGGCAC 1101 AAGCAAACTG TCTGCGCGTT TCGGTGCCGA AGAAATGTAT GACTTCTATC 1151 ATGAGTTGGG CATCGGTGTG CGTATGCACT CGGGCTTTCC GGGCGAAACT 1201 GCAGGTTTGT TGAGAAATTG GCGCAGGTGG CGGCCTATCG AACAGGCGAC 1251 GATGTCTTTC GGTTACGGCC TGCAATTGAG CCTGCTGCAA TTGGCGCGCG 1301 CCTATACCGC ACTGACGCAC GACGGCGTTT TACTGCCGGT CAGCTTTGAA 1351 AAACAGGCGG TTGCGCCGCA AGGCAAACGC ATATTCAAAG AATCGACCGC 1401 GCGCGAGGTA CGCAATCTGA TGGTTTCCGT AACCGAGCCG GGCGGCACCG 1451 GTACGGCGGG TGCGGTGGAC GGTTTCGATG TCGGCGCGAA AACCGGCACG 1501 GCGCGCAAGT TCGTCAACGG GCGTTATGCC GACAACAAAC ACATCGCTAC 1551 CTTTATCGGT TTTGCCCCCG CCAAAAATCC CCGTGTGATT GTGGCGGTAA 1601 CCATTGACGA ACCGACTGCC CACGGTTATT ACGGCGGCGT AGTGGCAGGG 1651 CCGCCCTTCA AAAAAATTAT GGGCGGCAGC CTGAACATCT TGGGCATTTC 1701 CCCGACCAAG CCACTGACCG CCGCAGCCGT CAAAACACCG TCTTAA This corresponds to the amino acid sequence <SEQ ID 2692; ORF 793. a>: a793.pep 1 MLIKSEYKPR MLPKEEQVKK PMTSΝGRISF VLMAIAVLFA GLIARGLYLQ 51 TVTYΝFLKEQ GDΝRIVRTQT LPATRGTVSD RΝGAVLALSA PTESLFAVPK 101 EMKEMPSAAQ LERLΞELVDV PVDVLRΝKLE QKGKSFIWIK RQLDPKVAEE 151 VKALGLEΝFV FEKELKRHYP MGΝLFAHVIG FTDIDGKGQE GLELSLEDSL 201 HGEDGAEWL RDRQGΝIVDS LDSPRΝKAPK ΝGKDIILΞLD QRIQTLAYEE 251 LΝKAVEYHQA KAGTVWLDA RTGEILALAΝ TPAYDPΝRPG RADSEQRRΝR 301 AVTDMIEPGS AIKPFVIAKA LDAGKTDLΝE RLΝTQPYKIG PSPVRDTHVY 351 PSLDVRGIMQ KSSΝVGTSKL SARFGAEEMY DFYHELGIGV RMHSGFPGET 401 AGLLRΝWRRW RPIEQATMSF GYGLQLΞLLQ LARAYTALTH DGVLLPVSFE 451 KQAVAPQGKR IFKESTAREV RΝLMVSVTEP GGTGTAGAVD GFDVGAKTGT 501 ARKFVΝGRYA DΝKHIATFIG FAPAKΝPRVI VAVTIDEPTA HGYYGGWAG 551 PPFKKIMGGS LΝILGISPTK PLTAAAVKTP S* a793/m793 100.0% identity in 581 aa overlap 10 20 30 40 50 60 a793. •pep MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIARGLYLQTVTYNFLKEQ I I I I II II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m793 MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIARGLYLQTVTYNFLKEQ 10 20 30 40 50 60 70 80 90 100 110 120 a793. •pep GDNRIVRTQTLPATRGTVSDRNGAVLALSAPTEΞLFAVPKEMKEMPSAAQLERLSELVDV II I I II I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m793 GDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEMKEMPSAAQLERLSELVDV 70 80 90 100 110 120 130 140 150 160 170 180 a793. ■ pep PVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFVFEKELKRHYPMGNLFAHVIG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m793 PVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFVFEKELKRHYPMGNLFAHVIG 130 140 150 160 170 180 190 200 210 220 230 240 a793, .pep FTDIDGKGQEGLELSLEDSLHGEDGAEWLRDRQGNIVDSLDSPRNKAPKNGKDI ILSLD I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m793 FTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIVDSLDSPRNKAPKNGKDIILSLD 190 200 210 220 230 240 250 260 270 280 290 300 a793. .pep QRIQTLAYEELNKAVEYHQAKAGTVWLDARTGEILALANTPAYDPNRPGRADSEQRRNR I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m793 QRIQTLAYEELNKAVEYHQAKAGTVWLDARTGEILALANTPAYDPNRPGRADSEQRRNR 250 260 270 280 290 300 310 320 330 340 350 360 a793. ■ pep AVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNTQPYKIGPSPVRDTHVYPSLDVRGIMQ I II I II I I I I I I I II I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II m793 AVTDMIEPGΞAIKPFVIAKALDAGKTDLNERLNTQPYKIGPSPVRDTHVYPSLDVRGIMQ 310 320 330 340 350 360 370 380 390 400 410 420 a793. pep KSSNVGTSKLSARFGAEEMYDFYHELGIGVRMHSGFPGETAGLLRNWRRWRPIEQATMSF I II I II I I I I I I I II I I I I II I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m793 KSΞNVGTSKLΞARFGAEEMYDFYHELGIGVRMHΞGFPGETAGLLRNWRRWRPIEQATMSF 370 380 390 400 410 420 430 440 450 460 470 480 a793. .pep GYGLQLSLLQLARAYTALTHDGVLLPVSFEKQAVAPQGKRIFKESTAREVRNLMVΞVTEP I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m793 GYGLQLSLLQLARAYTALTHDGVLLPVΞFEKQAVAPQGKRIFKESTAREVRNLMVSVTEP 430 440 450 460 470 480 490 500 510 520 530 540 a793.pep GGTGTAGAVDGFDVGAKTGTARKFVNGRYADNKHIATFIGFAPAKNPRVIVAVTIDEPTA I II I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m793 GGTGTAGAVDGFDVGAKTGTARKFVNGRYADNKHIATFIGFAPAKNPRVIVAVTIDEPTA 490 500 510 520 530 540 550 560 570 580 a793 . pep HGYYGGWAGPPFKKIMGGSLNILGIΞPTKPLTAAAVKTPSX I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m793 HGYYGGWAGPPFKKIMGGΞLNILGISPTKPLTAAAVKTPSX 550 560 570 580 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2693>: g794.seq 1 gtgcgtttca ATCATTTCAT AATGGTAACG ATTATTATAT ATGTGATTTC 51 CCCTGCAAAC AAGCCGGTCC GCCGCCCCGG CGTTCCCACT TATCCGGCTT 101 TGCCTTATAA TTGCTTTTTT TATGTAACAG ATTCACCTAT GAATTTCCCC 151 AAAACAGCGG CCTCCCTGCT GCTGCTTCTC GCCTCCCTCG CCGCACACGC 201 GCTCGATACC GGCCGCATTC CGCAAAACGA AATCGCTGTA TATGTCCAAG 251 AGCTTGACAG CGGAAAAGTC ATCATTGACC ACCGTGCCGG CATACCCGTC 301 AATCCCGCGT CCACGATGAA GCTCGTTACC GCGTTTGCCG CCTTCAAAAC 351 CTTCGGCAGC AATTACCGCT GGGCGACCGA GTTTAAAAGC AACGGTACGG 401 TAAACGACGG CACGCTTGAC GGAAACCTGT ATTGGGCGGG CAGCGGCGAC 451 CCCGTTTTCA ATCAGGAAAA CCTGCTTGCC GTCCAACGCC AGTTGCGCGA 501 CAAAGGCATC CGCAATATCA CGGGGCGCCT GATGCTCGAC CACAGCCTGT 551 GGGGCGAAGT CGGCAGTCCC GACCATTTTG AAGCCGACAG CGGTTCGCCG 601 TTTATGACGC CCCCAAATCC GACTATGCTG TCTGCCGGTA TGGTTATGGT 651 GCGCGCCGAA CGCAATGCCG CCGGCAGTAC CGACATCCTC ACCGATCCGC 701 CTTTGCCGCA TATTTTTGCC CAAAACAACT TGAAAATTAC CGCCTCCCAA 751 GCTGCCTGCC CTTCGGTCAA AAAACTGATG CGCGCATCTT TTTCGGGCAA 801 TACGCTGAAA TTGCGCGGCA ATATTCCCGA AAGCTGTTTG GGCAAGCCTG 851 TCGGTGTCCG GATGTTCGCG CTTGACGAAC TGATCCGCCA AAGTTTTACC 901 AACCGCTGGC TGCTCGGCGG CGGACGGATT TCAGACGGCA TCGGCATAGC 951 CGACACACCG GAAGGCGCGC AGACGCTTGC CGTTGCACAC TCCAAACCGA 1001 TGAAGGAAAT TTTGACGGAC ATGAACAAGC GTTCGGACAA TCTGATTGCG 1051 CGTTCCGTCT TCCTCAAACT CGGCGGCGAC GGCAAACTGC CCGCCGTTTC 1101 CGAACAGGCG GCGTCTGCCG TCCGGCGAGA ACTTGCCGTA TCGGGCATCG 1151 ATGTTGCGGA TTTGGTTTTG GAAAACGGTT CGGGTCTGTC CAGAAAAGAA 1201 AGGGTAACGG CGAGAATGAT GGCGCAAATG TTGGAAACGG CTTATTTCAG 1251 CCCGTTTGCA CAAGATTTCA TCGACACGCT GCCCATCGCC GGCACAGACG 1301 GGACTTTACG CAACCGCTTC AAACAAAGCG GCGGGCTGTT GCGCTTAAAA 1351 ACCGGCACGC TCAACAATGT CCGCGCCCTT GCAGGTTATT GGCTGGGCGA 1401 CAAACCGATG GCGGTGGTCG TCATCATCAA CAGCGGCCGC GCCGTTTCCC 1451 TGCTGCCCGA CTTGGACAAC TTCGTTGCCA AAAACATCAT CTCCGGCGGC 1501 GACGGCTGGC TGGATGCGAA ACTGATGTGC AAAGAACGCC GCGCCTGA This corresponds to the amino acid sequence <SEQ ID 2694; ORF 794.ng>: g794.pep 1 VRFNHFIMVT IIIYVIΞPAN KPVRRPGVPT YPALPYNCFF YVTDSPMNFP 51 KTAASLLLLL ASLAAHALDT GRIPQNEIAV YVQELDSGKV IIDHRAGIPV 101 NPAΞTMKLVT AFAAFKTFGS NYRWATEFKS NGTVNDGTLD GNLYWAGSGD 151 PVFNQENLLA VQRQLRDKGI RNITGRLMLD HSLWGEVGSP DHFEADSGSP 201 FMTPPNPTML SAGMVMVRAE RNAAGSTDIL TDPPLPHIFA QNNLKITASQ 251 AACPSVKKLM RASFSGNTLK LRGNIPESCL GKPVGVRMFA LDELIRQΞFT 301 NRWLLGGGRI SDGIGIADTP EGAQTLAVAH SKPMKEILTD MNKRSDNLIA 351 RSVFLKLGGD GKLPAVSEQA ASAVRRELAV SGIDVADLVL ENGSGLSRKE 401 RVTARMMAQM LETAYFSPFA QDFIDTLPIA GTDGTLRNRF KQΞGGLLRLK 451 TGTLNNVRAL AGYWLGDKPM AVVVIINSGR AVSLLPDLDN FVAKNIISGG 501 DGWLDAKLMC KERRA* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2695>: m794.seq 1 GTGCGTCTCA ATCATTTCAT AATGATAGCG ATTATTATAT ATGTGATTTC 51 CCCTGCAAAC AAGCCGGCCC GCCGCCACAG CGTTCCCACT TATCCGGCTT 101 TGCCTTATAA TTGCTTTTTT TATGTAACAG ATTTACCTAT GAATTTCCCC 151 AAAACAGCGG CCTCCCTGCT GCTGCTTCTC GCCTCCCTCG CCGCACACGC 201 GCTCGATACC GGCCGCATTC CGCAAAACGA AATCGCCGTA TATGTCCAAG 251 AGCTTGACAG CGGAAAAGTC ATCATTGACC ACCGCTCGGA TGTCCCCGTC 301 AACCCCGCCT CCACAATGAA ACTCGTTACC GCGTTTGCCG CCTTCAAAAC 351 CTTCGGCAGC AATTACCGCT GGGCGACCGA GTTTAAAAGC AACGGTACGG 401 TAAACGACGG CACGCTTGAC GGAAACCTAT ATTGGGCGGG CAGCGGCGAC 451 CCCGTTTTCA ATCAGGAAAA CCTGCTTGAT GCTCAAAAAC AGTTGCGCGA 501 ACAAGGCATA CTCAATATCA CGGGACACCT GATGCTCGAC CACAGCCTGT 551 GGGGCGAAGT CGGCAGCCCC GACGATTTCG AAGCCGACAG CGGTTCGCCG 601 TTTATGACGC CCCCCAATCC AACTATGCTG TCTGCCGGTA TGGTTATGGT 651 GCGCGCCGAA CGCAATGCCG CCGGCAGTAC CGACATCCTC ACCGATCCGC 701 CTTTGCCGCA TATTTTCGCC CAAAACAACT TGAAAATTAC CGCCTCCCAA 751 GCTGCCTGCC CTTCGATCAA AAAACTGATG CGTGCATCTT TTTCGGACAA 801 TACGCTGAAA TTGCGCGGCA ATATTCCCGA GAGCTGTTTG GGCAAGCCTG 851 TCGGTGTCCG GATGTTCGCG CTTGACGAAC TGATCCGGCA AAGTTTTACC 901 AACCACTGGC TGCTCGGCGG CGGACGGATT TCAGACGGTA TCGGCATAGC 951 CGACACGCCG GAAGGCGCGC AGACACTTGC CGTTGCACAC GCCAAACCGA 1001 TGAAAGAAAT TTTGACGGAC ATGAACAAGC GTTCGGACAA TCTAATTGCG 1051 CGTTCCGTCT TCCTCAAACT CGGCGGCGAC GGCAAACTGC CCGCCGTTTC 1101 CGAACAGGCG GCGTCTGCCG TCCGGCGCGA ACTTGCCGTA TCGGGCATCG 1151 ATGTTGCGGA TTTGGTTTTG GAAAACGGTT CGGGCCTGTC CAGAAAAGAA 1201 AGGGTAACGG CGAGAATGAT GGCGCAAATG TTGGAAACGG CTTATTTCAG 1251 CCCGTTTGCA CAAGATTTCA TCGACACGCT ACCCATCGCC GGCACAGACG 1301 GAACTTTACG CAACCGCTTC AAACAAAGCG GCGGGCTGTT GCGCTTAAAA 1351 ACCGGCACGC TCAACAATGT CCGCGCCCTT GCAGGTTATT GGCTGGGCGA 1401 CAAACCGATG GCGGTGGTCG TCATCATCAA CAGCGGCCGC GCCGTTTCCC 1451 TGCTGCCAGA CTTGGACAAC TTCGTTGCCA ACAACATCAT CTCCGGCGGC 1501 GATGGCTGGC TGGATGCGAA ACTGATGTGC AAAGAACGCC GAGCCTGA This corresponds to the amino acid sequence <SEQ ID 2696; ORF 794>: m794.pep 1 VRLΝHFIMIA IIIYVISPAN KPARRHSVPT YPALPYNCFF YVTDLPMNFP 51 KTAASLLLLL ASLAAHALDT GRIPQNEIAV YVQELDSGKV IIDHRΞDVPV 101 NPASTMKLVT AFAAFKTFGS NYRWATEFKS NGTVNDGTLD GNLYWAGSGD 151 PVFNQENLLD AQKQLREQGI LNITGHLMLD HSLWGEVGSP DDFEADΞGSP 201 FMTPPNPTML SAGMVMVRAE RNAAGΞTDIL TDPPLPHIFA QNNLKITASQ 251 AACPSIKKLM RASFSDNTLK LRGNIPESCL GKPVGVRMFA LDELIRQSFT 301 NHWLLGGGRI SDGIGIADTP EGAQTLAVAH AKPMKEILTD MNKRSDNLIA 351 RSVFLKLGGD GKLPAVSEQA ASAVRRELAV SGIDVADLVL ENGSGLΞRKE 401 RVTARMMAQM LETAYFSPFA QDFIDTLPIA GTDGTLRNRF KQSGGLLRLK 451 TGTLNNVRAL AGYWLGDKPM AVWIINSGR AVSLLPDLDN FVANNIISGG 501 DGWLDAKLMC KERRA* g794/ 794 95.5% identity in 515 aa overlap 10 20 30 40 50 60 g794.pep VRFNHFIMVTIIIYVISPANKPVRRPGVPTYPALPYNCFFYVTDSPMNFPKTAASLLLLL I M I I I I MM I I II I I I I I I M I I : I I I I I I I I I I I I I I I I I m794 VRLNHFIMIAI11YVISPANKPARRHSVPTYPALPYNCFFYVTDLPMNFPKTAASLLLLL 10 20 30 40 50 60 70 80 90 100 110 120 g794.pep ASLAAHALDTGRIPQNEIAVYVQELDSGKVIIDHRAGIPVNPASTMKLVTAFAAFKTFGS I II I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I : : I I I I I I I I I I I I I I I I I I I I I I m794 ASLAAHALDTGRIPQNEIAVYVQELDSGKVIIDHRSDVPVNPASTMKLVTAFAAFKTFGΞ 70 80 90 100 110 120 130 140 150 160 170 180 g794.pep NYRWATEFKSNGTVNDGTLDGNLYWAGΞGDPVFNQENLLAVQRQLRDKGIRNITGRLMLD I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I : I I I :: I I I I I I : I I I I m794 NYRWATEFKSNGTVNDGTLDGNLYWAGSGDPVFNQENLLDAQKQLREQGILNITGHLMLD 130 140 150 160 170 180 190 200 210 220 230 240 g794.pep HSLWGEVGSPDHFEADSGSPFMTPPNPTMLΞAGMVMVRAERNAAGSTDILTDPPLPHIFA I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m794 HSLWGEVGSPDDFEADSGΞPFMTPPNPTMLΞAGMVMVRAERNAAGSTDILTDPPLPHIFA 190 200 210 220 230 240 250 260 270 280 290 300 g794.pep QNNLKITASQAACPSVKKLMRASFSGNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFT I I I I II I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m794 QNNLKITASQAACPΞIKKLMRASFSDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFT 250 260 270 280 290 300 310 320 330 340 350 360 g794.pep NRWLLGGGRIΞDGIGIADTPEGAQTLAVAHSKPMKEILTDMNKRΞDNLIARSVFLKLGGD I : I I II I I I I I I I I I I I I I I I I I I I I I I I Ml I I I I I I I I I I I I I I I I I I I I I I I I I I I I m794 NHWLLGGGRIΞDGIGIADTPEGAQTLAVAHAKPMKEILTDMNKRSDNLIARSVFLKLGGD 310 320 330 340 350 360 370 380 390 400 410 420 g794.pep GKLPAVSEQAAΞAVRRELAVSGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFA I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m794 GKLPAVSEQAASAVRRELAVSGIDVADLVLENGSGLΞRKERVTARMMAQMLETAYFSPFA 370 380 390 400 410 420 430 440 450 460 470 480 g794.pep QDFIDTLPIAGTDGTLRNRFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVWIINΞGR I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m794 QDFIDTLPIAGTDGTLRNRFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVWIINSGR 430 440 450 460 470 480 490 500 510 g794.pep AVΞLLPDLDNFVAKNIISGGDGWLDAKLMCKERRAX I II I II I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I m794 AVSLLPDLDNFVANNIISGGDGWLDAKLMCKERRAX 490 500 510 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2697>: a794.seq 1 GTGCGTCTCA ATCATTTCAT AATGATAGCG ATTATTATAT ATGTGATTTC 51 CCCTGCAAAC AAGCCGGCCC GCCGCCACAG CGTTCCCACT TATCCGGCTT 101 TGCCTTATAA TTGCTTTTTT TATGTAACAG ATTTACCTAT GAATTTCCCC 151 AAAACAGCGG CCTCCCTGCT GCTGCTTCTC GCCTCCCTCG CCGCACACGC 201 GCTCGATACA GGTCGCATTC CGCAAAACGA AATCGCCGTA TATGTCCAAG 251 AGCTTGACAG CGGAAAAGTC ATCATTGACC ACCGCTCGGA TGTCCCCGTC 301 AACCCCGCCT CCACAATGAA ACTCGTTACC GCGTTTGCCG CCTTCAAAAC 351 CTTCGGCAGC AATTACCGCT GGGCGACCGA GTTTAAAAGC AACGGTACGG 401 TAAACGACGG CACGCTTGAC GGAAACCTGT ATTGGGCGGG CAGCGGCGAC 451 CCCGTTTTCA ATCAGGAAAA CCTGCTTGCC GTCCAACGCC AGTTGCGCGA 501 ACAAGGCATA CGCAATATCA CGGGACACCT GATGCTCGAC CACAGCCTGT 551 GGGGCGAAGT CGGCAGCCCC GACGATTTCG AAGCCGACAG CGGTTCGCCG 601 TTTATGACGC CCCCCAATCC AACTATGCTG TCTGCCGGTA TGGTTATGGT 651 GCGCGCCGAA CGCAATGCCG CCGACAGTAC CGACATCCTC ACCGATCCGC 701 CTTTGCCGCA TATTTTCGCC CAAAACAACT TGAAAATTAC CGCCTCCCAA 751 GCTGCCTGCC CTTCGATCAA AAAACTGATG CGTGCATCTT TTTCGGACAA 801 TACGCTGAAA TTGCGCGGCA ATATTCCCGA GAGCTGTTTG GGCAAGCCTG 851 TCGGTGTCCG GATGTTCGCG CTTGACGAAC TGATCCGGCA AAGTTTTACC 901 AACCACTGGC TGCTCGGCGG CGGACGGATT TCAGACGGCA TCGGCATATC 951 CGACACGCCG GAAGGCGCGC AGACGCTTGC CGTTGCACAC TCAAAGCCGA 1001 TGAAGGAAAT TTTGACGGAC ATGAACAAGC GTTCGGACAA TCTAATTGCG 1051 CGTTCCGTCT TCCTCAAACT CGGCGGCGAC GGCAAACTGC CCGCCGTTTC 1101 CGAACAGGCA GCGTCTGCCG TCCGGCGTGA ACTTGCCGTG TCGGGCATCG 1151 ATGTTGCGGA TTTGGTTTTG GAAAACGGTT CAGGTCTGTC CAGAAAAGAA 1201 AGGGTAACGG CGAGAATGAT GGCGCAAATG TTGGAAACGG CTTATTTCAG 1251 CCCGTTTGCA CAAGATTTCA TCGATACGCT GCCCATCGCC GGCACAGACG 1301 GGACTTTACG CAACCGCTTC AAACAAAGCG GCGGGCTGTT GCGCTTAAAA 1351 ACCGGCACGC TCAACAATGT CCGCGCCCTT GCAGGTTATT GGCTGGGCGA 1401 CAAACCGATG GCGGTGGTCG TCATCATCAA CAGCGGCCGC GCCGTTTCCC 1451 TGCTGCCCGA CTTGGACAAC TTCGTTGCCA ACAACATCAT CTCCGGCGGC 1501 GATGGCTGGC TGGATGCGAA ACTGATGTGC AAAGAACGCC GAGCCTGA This corresponds to the amino acid sequence <SEQ ID 2698; ORF 794. a>: a794.pep 1 VRLNHFIMIA IIIYVIΞPAN KPARRHSVPT YPALPYNCFF YVTDLPMNFP 51 KTAASLLLLL ASLAAHALDT GRIPQNEIAV YVQELDSGKV IIDHRΞDVPV 101 NPASTMKLVT AFAAFKTFGS NYRWATEFKS NGTVNDGTLD GNLYWAGSGD 151 PVFNQENLLA VQRQLREQGI RNITGHLMLD HSLWGEVGSP DDFEADSGSP 201 FMTPPNPTML SAGMVMVRAE RNAADSTDIL TDPPLPHIFA QNNLKITASQ 251 AACPSIKKLM RASFSDNTLK LRGNIPESCL GKPVGVRMFA LDELIRQSFT 301 NHWLLGGGRI ΞDGIGISDTP EGAQTLAVAH SKPMKEILTD MNKRSDNLIA 351 RSVFLKLGGD GKLPAVSEQA ASAVRRELAV SGIDVADLVL ENGSGLSRKE 401 RVTARMMAQM LETAYFSPFA QDFIDTLPIA GTDGTLRNRF KQSGGLLRLK 451 TGTLNNVRAL AGYWLGDKPM AVWIINSGR AVSLLPDLDN FVANNIISGG 501 DGWLDAKLMC KERRA* a794/m794 98.6% identity in 515 aa overlap 10 20 30 40 50 60 a794.pep VRLNHFIMIAIIIYVIΞPANKPARRHSVPTYPALPYNCFFYVTDLPMNFPKTAASLLLLL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m794 VRLNHFIMIAIIIYVISPANKPARRHSVPTYPALPYNCFFYVTDLPMNFPKTAASLLLLL 10 20 30 40 50 60 70 80 90 100 110 120 a794.pep ASLAAHALDTGRIPQNEIAVYVQELDSGKVIIDHRΞDVPVNPASTMKLVTAFAAFKTFGS I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m794 ASLAAHALDTGRIPQNEIAVYVQELDΞGKVIIDHRΞDVPVNPASTMKLVTAFAAFKTFGS 70 80 90 100 110 120 130 140 150 160 170 180 a794.pep NYRWATEFKΞNGTVNDGTLDGNLYWAGSGDPVFNQENLLAVQRQLREQGIRNITGHLMLD I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M :| I I I I I I m794 NYRWATEFKSNGTVNDGTLDGNLYWAGΞGDPVFNQENLLDAQKQLREQGILNITGHLMLD 130 140 150 160 170 180 190 200 210 220 230 240 a794.pep HΞLWGEVGSPDDFEADΞGSPFMTPPNPTMLSAGMVMVRAERNAADSTDILTDPPLPHIFA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I Itι794 HSLWGEVGSPDDFEADΞGSPFMTPPNPTMLSAGMVMVRAERNAAGSTDILTDPPLPHIFA 190 200 210 220 230 240 250 260 270 280 290 300 a794.pep QNNLKITASQAACPSIKKLMRASFSDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFT 11111111111111 II M 111111111111111111111111111111111111111111 m794 QNNLKITASQAACPS IKKLMRAS FΞDNTLKLRGNI PESCLGKPVGVRMFALDELI RQΞ FT 250 260 270 280 290 300 310 320 330 340 350 360 a794 . pep NHWLLGGGRISDGIGISDTPEGAQTLAVAHSKPMKEILTDMNKRSDNLIARSVFLKLGGD I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I : I I I I I I I I I I I I I I i I I I I I I I I I I I I I I m794 NHWLLGGGRISDGIGIADTPEGAQTLAVAHAKPMKEILTDMNKRSDNLIARSVFLKLGGD 310 320 330 340 350 360 370 380 390 400 410 420 a794.pep GKLPAVSEQAASAVRRELAVSGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m794 GKLPAVΞEQAASAVRRELAVSGIDVADLVLENGΞGLSRKERVTARMMAQMLETAYFΞPFA 370 380 390 400 410 420 430 440 450 460 470 480 a794.pep QDFIDTLPIAGTDGTLRNRFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m794 QDFIDTLPIAGTDGTLRNRFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVWIINSGR 430 440 450 460 470 480 490 500 510 a794.pep AVSLLPDLDNFVANNIISGGDGWLDAKLMCKERRAX I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I tl794 AVSLLPDLDNFVANNIISGGDGWLDAKLMCKERRAX 490 500 510 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 2699>: g900.seq 1 ATGccgTCTG AAATGCCGTC TGAAACGTGG CAGGCGGAGG TTCGGACGGC 51 ATTGGGTTTA TTTCAACGGG CGGATGCCGA CCGCATCGCG TACTTTATCC 101 AACAATTCGC GCGCTTCTTT GCGCGCTTTT TGCGCGcctg cctGCAAAAT 151 CTCTTCGATT TGCGAAGGAT TAGAGGTCAA TGCGTTGTAG CGTTCGCGCA 201 GTTCTGCCAA TTCGGCGTTG ATTTTCGCCG CCGAAAGTTT TTTCGCCTCG 251 CCCCAAGCCA AGCCGTCGGC AAGCATTTGC GTAAATTCCG CCGTTTCAGA 301 CGGCGTGGAG AAGGCTTTAT AGATTTCAAA CAAAGGGCTT TCGTCGGGCT 351 GTTTCGGCTC GCCCGGCTCT TTCATGTTGG TAATGATTTT GTTGACCGAT 401 TTTTGGGTTT TTTTGTCGTT TTCCCAAAGC GGAATGGTAT TGCCGTAGGA 451 TTTGGACATT TTGCGTCCGT CCAAACCGAC CAAGAGTTCG ACGTTTTCGT 501 CGATTTTCAC TTCGGGCagg GTGaagagtt cTTGGAaacc gtgggtgaag 551 cggccggcAa tgtcgcgcgc cATTTcgacg tgttgGATTT GGTCGCGCCC 601 GACGGGGACT TCGTTGGCGT TGAACATCAA AATGTCGGCA GTCATCAGAA 651 TCGGATAACT GAACAAACCC ATTTCCACAC CGAAATCGGG GTCTTCCTGC 701 CCGTTTTCCG CATTGGCTTG AACGGCGGCT TTGTAGGCGT GGGCGCGGTT 751 CATCAAACCC TTGGCGGTGA TGCAGGTCAG AATCCAGTTC AACTCCATCA 801 CTTCGGGAAT GTCGCTTTGG CGGTAGAAGG TGGTGCGCTC GGGGTCGAGT 851 CCGCAGGCAA GCCAAGTGGC GGCAACGGCt tgGGTGGATT GGTGAATCAT 901 CTCCTGCTCG TGGCATTTGA TGATGCCGTG GTAATCGGCG AGGAAGAGGA 951 AGGATTCGGT ATCGGGGTTT TGCGCCGCGC GGACGGCGGG GCGGATGGCG 1001 CCGACGTAGT TGCCCAGATG CGGGGTGCCG GTGGTGGTTA CGCCGGTCAG 1051 AACTCGTTTT TTGCTCATAA AAATGTCCTT ACGGCAGCAA TGCCGTCTGA 1101 AAGGGAAAa. gatgcgCCGA TTATACCCGA TTTGCCACAT ACATCCAGCC 1151 GacaACagaC TTTTCCATAT TAA This corresponds to the amino acid sequence <SEQ ID 2700; ORF 900.ng: g900.pep 1 MPSEMPSETW QAEVRTALGL FQRADADRIA YFIQQFARFF ARFLRACLQΝ 51 LFDLRRIRGQ CWAFAQFCQ FGVDFRRRKF FRLAPSQAVG KHLRKFRRFR 101 RRGEGFIDFK QRAFVGLFRL ARLFHVGΝDF VDRFLGFFW FPKRΝGIAVG 151 FGHFASVQTD QEFDVFVDFH FGQGEEFLET VGEAAGΝVAR HFDVLDLVAP 201 DGDFVGVEHQ ΝVGSHQΝRIT EQTHFHTEIG VFLPVFRIGL ΝGGFVGVGAV 251 HQTLGGDAGQ ΝPVQLHHFGΝ VALAVEGGAL GVESAGKPSG GΝGLGGLVΝH 301 LLLVAFDDAV VIGEEEEGFG IGVLRRADGG ADGAD AQM RGAGGGYAGQ 351 ΝSFFAHKΝVL TAAMPSEREK DAPIIPDLPH TSSRQQTFPY * The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2701>: m900.seq 1 ATGCCGTCTG AAACGCGGCA GGCGGAGGTT CGGACGGCAT CGGGTTCATT 51 TCAACGGGCG GATGcCGACC GCATCgG.TA CTTTGTCCAA TAATTCGCGT 101 GCTTCTTTAC GCGCTTTCGC CGCGCCTGCC TGCAAAATCT CTTCGATTTG 151 CGAAGGGTCG GCGGTCAGCT CGTTGTAGCG TTCGCGCGGT TCGGCGAGTT 201 CGGCGTTGAT TTTCGCCGCC AAAAGTTTTT TGGCTTCACC CCACGCCAAG 251 CCGTCGGCAA GCATTTTCGT AAATTCCACC GTTTCAGACG GCGTGGAGAA 301 GGCTTTGTAG ATTTCAAACA ATGGGCTTTC GTCGGGCTGT TTCGGCTCGC 351 CCGGCTCTTT CATATTGGTG ATGATTTTGT TGACCGATTT TTGGGTTTTT 401 tTGTCGTTTT CCCAAAGCGG AATGGTGTTG CCGTAGGATT TGGACATTTT 451 GCGTCCGTCC AAACCGACCA AGAGTTCGAC GTTTTCATCG ATTTTCACTT 501 CGGGCAGGGT GAAGAGTTCC CGGAAGCGGT GGTTGAAGCG GCCGGCGATG 551 TCGCGCGCCA TTTCGACGTG TTGGATTTGG TCGCGCCCGA CgGGCaCTTC 601 GTTGGCGTTG AACATCAGAA TATCGGCAGT CATCAGAATC GGATAACTGA 651 ACAAACCCAT TTCCACACCG AAATCAGGGT CTTCCTGCCC GTTTTCTGCA 701 TTTGCCTGCA CGGCGGCTTT GTAGGCATGG GCGCGGTTCA TCAAACCCTT 751 GGCAGTGATG CAGGTCAGAA TCCAGTTCAA TTCCATCACT TCgGGAGTGT 801 CGCTTTGGCG GTAGAAGGTG GTGCGCTCGG GGTCGAGTCC GCAgGCAAGC 851 CAAGTGGCGG CAACGGCTTG GGTGGATTGG TGAATCATCT CCGGCTCGTG 901 GCATTTGATG ATACCGTGGT AATCGGCGAG GAAGAGGAAG GATTCGGTAT 951 CGAGGTTTTG CGCCGCGCGG ACGGCGGGGC GGATGGCGCC GACGTAGTTG 1001 CCCAGATGCG GGATGCCGGT GGTGGTTACG CCGGTCAGAA CTCGTTTTTT 1051 GCTCATAAAA ATGTCCTTGC GGCATCAATG CCGTCTGAAA GGGAAAAAGA 1101 TGTGCCGATT ATACCCGATT TGCCACCTAC ATCCAGCCGA CAACAGACTT 1151 TTCCATATTA A This corresponds to the amino acid sequence <SEQ ID 2702; ORF 900>: m900.pep 1 MPSETRQAEV RTASGSFQRA DADRIXYFVQ *FACFFTRFR RACLQNLFDL 51 RRVGGQLWA FARFGEFGVD FRRQKFFGFT PRQAVGKHFR KFHRFRRRGE 101 GFVDFKQWAF VGLFRLARLF HIGDDFVDRF LGFF FPKR NGVAVGFGHF 151 ASVQTDQEFD VFIDFHFGQG EEFPEA EA AGDVARHFDV LDLVAPDGHF 201 VGVEHQNIGS HQNRITEQTH FHTEIRVFLP VFCICLHGGF VGMGAVHQTL 251 GSDAGQNPVQ FHHFGSVALA VEGGALGVES AGKPSGGNGL GGLVNHLRLV 301 AFDDTWIGE EEEGFGIEVL RRADGGADGA D AQMRDAG GGYAGQNSFF 351 AHKNVLAASM PSEREKDVPI IPDLPPTSSR QQTFPY* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 900 shows 87.0% identity over a 386 aa overlap with a predicted ORF (ORF 900.ng) from N gonorrhoeae: m900/g900 10 20 30 40 50 m900. ep MPSETRQAEVRTASGSFQRADADRIGYFVQXFACFFTRFRRACLQΝLFDLRRVGGQ M i l l I M I M M I l l M I l l I I g900 MPSEMPSETWQAEVRTALGLFQRADADRIAYFIQQFARFFARFLRACLQΝLFDLRRIRGQ 10 20 30 40 50 60 60 70 80 90 100 110 m900.pep LWAFARFGEFGVDFRRQKFFGFTPRQAVGKHFRKFHRFRRRGEGFVDFKQWAFVGLFRL llll l :|IIIM|:!II - 111111 = 11 h I 11111111 = 1111 g900 CWAFAQFCQFGVDFRRRKFFRLAPSQAVGKHLRKFRRFRRRGEGFIDFKQRAFVGLFRL 70 80 90 100 110 120 120 130 140 150 160 170 m900. pep ARLFHIGDDFVDRFLGFFWFPKRΝGVAVGFGHFASVQTDQEFDVFIDFHFGQGEEFPEA h g900 ARLFHVGΝDFVDRFLGFFWFPKRΝGIAVGFGHFASVQTDQEFDVFVDFHFGQGEEFLET 130 140 150 160 170 180 180 190 200 210 220 230 m900 . pep WEAAGDVARHFDVLDLVAPDGHFVGVEHQΝIGSHQΝRITEQTHFHTEIRVFLPVFCICL I l l l l l M M I I I M I I I I 1 1 1 I I 1 1 h 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 I I g900 VGEAAGΝVARHFDVLDLVAPDGDFVGVEHQΝVGSHQΝRITEQTHFHTEIGVFLPVFRIGL 190 200 210 220 230 240 240 250 260 270 280 290 m900. pep HGGFVGMGAVHQTLGSDAGQNPVQFHHFGSVALAVEGGALGVESAGKPSGGNGLGGLVNH g900 NGGFVGVGAVHQTLGGDAGQNPVQLHHFGNVALAVEGGALGVESAGKPSGGNGLGGLVNH 250 260 270 280 290 300 300 310 320 330 340 350 m900 . pep LRLVAFDDTWIGEEEEGFGIEVLRRADGGADGADWAQMRDAGGGYAGQNSFFAHKNVL I l l l l l l l l l l l l l l l l l 1 1 1 j E 1 1 1 1 1 1 1 1 I I I ! j i 1 1 1 1 1 F 1 1 1 1 i 1 1 1 1 [ 1 1 g900 LLLVAFDDAWIGEEEEGFGIGVLRRADGGADGADWAQMRGAGGGYAGQNSFFAHKNVL 310 320 330 340 350 360 360 370 380 m900 . pep AASMPSEREKDVPI IPDLPPTSSRQQTFPYX l l l l l l l l l l l l l l 1 1 1 1 J I ! i 1 1 1 g900 TAAMPSEREKDAPIIPDLPHTSSRQQTFPYX 370 380 390 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2703>: a900.sβq (partial) 1 GAGGTTCGGA CGGCATTGGG TTTATTTCAA CGGGCGGATA CCGACCGCAT 51 CACGTACTTT GCCCAATAAT TCGCGTGCTT CTTTACGCGC TTTTTGCGCG 101 CCTGCCTGCA AAATCTCTTC GATTTGCGAA GGGTCGGCGG TCAGCTCGTT 151 GTAGCGTTCG CGCGGTTCGG CGAGTTCGGC GTTGATTTTC GCCGCCAAAA 201 GTTTTTTTGC CTCGCCCCAA GCCAAGCCGT CGGCAAGCAT TTTCGTAAAT 251 TCTGCCGTTT CAGACGGCGT GGAGAAAGCT TTGTAGATTT CAAACAGAGG 301 GCTTTCGTCG GGCTTCTTCG GCTCGCCCGG CTCTTTCATA TTGGTGATGA 351 TTTTGTTGAC CGATTTTTGG GTTTTTTTGT CGTTTTCCCA AAGCGGAATG 401 GTGTTGCCGT AGGATTTGGA CATTTTGCGT CCGTCCAAAC CAACCAAGAG 451 TTCGACGTTT TCGTCGATTT TCACTTCGGG CAGTGTGAAG AGTTCCCGGA 501 AGCGGTGGTT GAAGCGGCCG GCAATATCGC GTGCCATTTC AACGTGTTGG 551 ATTTGGTCGC GACCGACTGG AACTTCATGG GCATTGAACA TGAGAATGTC 601 GGCAGTCATG AGGATAGGGT AGCTGTACAA ACCCATTTCC ACGCCGAAAT 651 CGGGGTCTTC CTGCCCGTTT TCCGCATTTG CCTGCACGGC GGCTTTGTAG 701 GCGTGGGCGC GGTTCATCAA ACCCTTGGCG GTGATGCAGG TCAGAATCCA 751 GTTCAATTCC ATCACTTCGG GAATGTCGCT TTGACGGTAG AAGGTGGTGC 801 GCTCGGGGTC GAGTCCGCAG GCAAGCCAAG TGGCGGCAAC GGCTTGGGTG 851 GATTGGTGAA TCATCTCCGG CTCGTGGCAT TTGATGATAC CGTGGTAATC 901 GGCGAGGAAG AGGAAGGATT CGGTATCAGG GTTTTGCGCC GCGCGGACGG 951 CGGGGCGGAT AGCACCGACG TAGTTGCCCA GATGCGGGAT GCCGGTGGTG 1001 GTTACGCCGG TCAGAACTCG TTTTTTGCTC ATAAAAATGT CCTTGCGGCA 1051 TCAATGCCGT CTGAAAGGGA AAAAGATGCG CCGATTATAC CCGATTTGCC 1101 ACCTACATCC AGCCGACAAC AGACTTTTCC ATATTAA This corresponds to the amino acid sequence <SEQ ID 2704; ORF 900.a>: a900.pep (partial) 1 EVRTALGLFQ RADTDRITYF AQ*FACFFTR FLRACLQΝLF DLRRVGGQLV 51 VAFARFGEFG VDFRRQKFFC LAPSQAVGKH FRKFCRFRRR GESFVDFKQR 101 AFVGLLRLAR LFHIGDDFVD RFLGFFWFP KRΝGVAVGFG HFASVQTΝQE 151 FDVFVDFHFG QCEEFPEAVV EAAGΝIACHF ΝVLDLVATDW ΝFMGIEHEΝV 201 GSHEDRVAVQ THFHAEIGVF LPVFRICLHG GFVGVGAVHQ TLGGDAGQΝP 251 VQFHHFGΝVA LTVEGGALGV ESAGKPSGGΝ GLGGLVΝHLR LVAFDDTWI 301 GEEEEGFGIR VLRRADGGAD STDWAQMRD AGGGYAGQΝS FFAHKΝVLAA 351 SMPSEREKDA PIIPDLPPTS SRQQTFPY* m900/a900 88.4% identity in 378 aa overlap 10 20 30 40 50 60 m900. pep MPSETRQAEVRTASGSFQRADADRIXYFVQXFACFFTRFRRACLQΝLFDLRRVGGQLVVA Mill I II II M II M II : II II II II I I II I I II I I I I I I II II I II I a900 EVRTALGLFQRADTDRITYFAQXFACFFTRFLRACLQΝLFDLRRVGGQLWA 10 20 30 40 50 70 80 90 100 110 120 m900. pep FARFGEFGVDFRRQKFFGFTPRQAVGKHFRKFHRFRRRGEGFVDFKQWAFVGLFRLARLF II II I I II II I I II I II :M I II I II II I I II I I I II: I I I I I I I I II M II II II a900 FARFGEFGVDFRRQKFFCLAPSQAVGKHFRKFCRFRRRGESFVDFKQRAFVGLLRLARLF 60 70 80 90 100 110 130 140 150 160 170 180 m900. pep HIGDDFVDRFLGFFWFPKRNGVAVGFGHFASVQTDQEFDVFIDFHFGQGEEFPEA EA II I I II II II I II II II I II II II II II II I II I I : II I II M I I II I I I II II I II II a900 HIGDDFVDRFLGFFVVFPKRNGVAVGFGHFASVQTNQEFDVFVDFHFGQCEEFPEAWEA 120 130 140 150 160 170 190 200 210 220 230 240 m900.pep AGDVARHFDVLDLVAPDGHFVGVEHQNIGSHQNRITEQTHFHTEIRVFLPVFCICLHGGF IMM II : II II II I : I : M I M I : II I : : I : : IIIIIMI llllll lllllll a900 AGNIACHFNVLDLVATDWNFMGIEHENVGSHEDRVAVQTHFHAEIGVFLPVFRICLHGGF 180 190 200 210 220 230 250 260 270 280 290 300 m900.pep VGMGAVHQTLGSDAGQNPVQFHHFGSVALAVEGGALGVESAGKPSGGNGLGGLVNHLRLV 11:1 II II I I M I II II II II II II : I II : I I I I II II II I I I I II II I I II II II II II a900 VGVGAVHQTLGGDAGQNPVQFHHFGNVALTVEGGALGVESAGKPSGGNGLGGLVNHLRLV 240 250 260 270 280 290 310 320 330 340 350 360 m900. pep AFDDTWIGEEEEGFGIEVLRRADGGADGADVVAQMRDAGGGYAGQNSFFAHKNVLAASM II I II II II II II I II I : II II II II I I : : II II II I II II II I II II I I II I I II II II a900 AFDDTVVIGEEEEGFGIRVLRRADGGADSTDVVAQMRDAGGGYAGQNSFFAHKNVLAASM 300 310 320 330 340 350 370 380 m900.pep PSEREKDVPIIPDLPPTSSRQQTFPYX I I II II I: I I II II I II II II II II I I a900 PSEREKDAPIIPDLPPTSSRQQTFPYX 360 370 g901. seq not found yet g901.pep not found yet The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2705>: m901.seq 1 ATGCCCGATT TTTCGATGTC CAATTTGGCC GTTGCCTTTT CCATCACATT 51 GGCTGCCGGT TTGTTTACCG TATTAkGyAG TGGCTTGGTG ATGTTTTCCA 101 AAACGCCCAA TCCGCGTGTG TTGTCGTTTG GTTTGGCGTT TGCCGGCGGT 151 GCGATGGTAT ATGTTTCCCT GACGGAGATT TTCAGTAAGT CCAGCGAGGC 201 GTTCGCTGAA ATTTATGATA AAGACCACGC GTTTGCGGCG GCGACCATGG 251 CATTTTTGGC CGGGATGGGC GGCATTGCGC TGATTGACCG TCTGGTGCCG 301 AACCCGCATG AAACTTTAGA CGCGCAAGAC CCGTCGTTTC AAGAAAGCAA 351 ACGCCGCCAT ATCGCGCGAG TCGGCATGAT GGCGGCGTTT GCGATTACTG 401 CGCACAATTT CCCCGAAGGC TTGGCGACGT TTTTTGCCAC ATTGGAAAAT 451 CCAGCAGTCG GGATGCCTTT GGCCTTGGCG ATTGCCATCC ATAATATTCC 501 GGAGGGCATT TCCATCGCCG CGCCGGTTTA TTTTGCCACC CGCAGCCGTA 551 AGAAAACGGT GTGGGCGTGT CTGCTATCCG GCTTGGCCGA GCCGTTGGGG 601 GCGGCTTTGG GCTATTTGGT TTTGCAGCCG TTTTTGTCGC CTGCCGTGTT 651 TGGTTCGGTA TTCGGCGTGA TAGCCGGTGT GATGGTGTTT TTGGCGTTGG 701 ACGAGCTGnt GCCGGCTGCC AAACGCTATT CAGACGGCCA TGAAACCGTT 751 TACGGCCTGA CAACGGGTAT GGCGGTGATT GCCGTCAGCC TGGTATTGTT 801 CCATTTTTAA This corresponds to the amino acid sequence <SEQ ID 2706; ORF 901>: m901.pep 1 MPDFSMSNLA VAFSITLAAG LFTVLXSGLV FSKTPNPRV LSFGLAFAGG 51 AMVYVSLTEI FSKSSEAFAE IYDKDHAFAA ATMAFLAGMG GIALIDRLVP 101 NPHETLDAQD PSFQESKRRH IARVGMMAAF AITAHNFPEG LATFFATLEN 151 PAVGMPLALA IAIHNIPEGI SIAAPVYFAT RSRKKTVWAC LLSGLAEPLG 201 AALGYLVLQP FLSPAVFGSV FGVIAGVMVF LALDELXPAA KRYSDGHETV 251 YGLTTGMAVI AVSLVLFHF* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2707>: aθOl.seq 1 ATGCCCGATT TTTCGATGTC CAATTTGGCC GTTGCCTTTT CCATTACGTT 51 GGCTGCCGGT TTGTTTACCG TATTAGGCAG CGGCTTGGTG ATGTTTTCCA 101 AAACGCCCAA TCCGCGCGTG TTGTCGTTTG GTTTGGCATT TGCCGGCGGT 151 GCGATGGTGT ATGTTTCCCT GACGGAGATT TTCAGTAAGT CCAGCGAGGC 201 GTTCGCTGAA ATTTATGATA AAGACCACGC GTTTGCGGCG GCGACCATGG 251 CATTTTTGGC AGGGATGGGC GGCATTGCGC TGATTGACCG TCTGGTGCCG 301 AACCCGCATG AAACTTTAGA CGCGCAAGAC CCGTCGTTTC AAGAAAGCAA 351 ACGCCGCCAT ATCGCGCGAG TCGGCATGAT GGCGGCGTTT GCGATTACTG 401 CGCACAATTT CCCCGAAGGC TTGGCGACGT TTTTTGCCAC ATTGGAAAAT 451 CCAGCAGTCG GGATGCCTTT GGCCTTGGCG ATTGCCATCC ATAATATTCC 501 GGAGGGCATT TCCATCGCCG CGCCGGTTTA TTTTGCCACC CGCAGCCGTA 551 AGAAAACGGT GTGGGCGTGT CTGCTATCCG GCTTGGCCGA GCCGTTGGGG 601 GCGGCTTTGG GCTATTTGGT TTTGCAGCCG TTTTTGTCGC CTGCCGTGTT 651 TGGTTCGGTA TTCGGCGTGA TAGCCGGTGT GATGGTGTTT TTGGCGTTGG 701 ACGAGCTGCT GCCGGCTGCC AAACGCTATT CAGACGGCCA TGAAACCGTT 751 TACGGCCTGA CAATGGGCAT GGCGGTGATT GCCGTCAGCC TGGTATTGTT 801 CCATTTTTAA This corresponds to the amino acid sequence <SEQ ID 2708; ORF 901. a>: a901.pep 1 MPDFSMSNLA VAFSITLAAG LFTVLGSGLV MFSKTPNPRV LSFGLAFAGG 51 AMVYVSLTEI FSKSSEAFAE IYDKDHAFAA ATMAFLAGMG GIALIDRLVP 101 NPHETLDAQD PSFQESKRRH IARVGMMAAF AITAHNFPEG LATFFATLEN 151 PAVGMPLALA IAIHNIPEGI SIAAPVYFAT RSRKKTVWAC LLSGLAEPLG 201 AALGYLVLQP FLSPAVFGSV FGVIAGVMVF LALDELLPAA KRYSDGHETV 251 YGLTMGMAVI AVSLVLFHF* m901/a901 98.9% identity in 269 aa overlap 10 20 30 40 50 60 m901.pep MPDFSMSNLAVAFSITLAAGLFTVLXSGLVMFSKTPNPRVLSFGLAFAGGAMVYVSLTEI II II II I II II II II II II II I II I I I II I I I II I II II II II II II I II II II II I II a901 M MPPDDFFSSMMSSNNLLAAVVAAFFSSIITTLLAAAAGGLLFFTTVVLLGGSSGGLLVVMMFFSSKKTTPPNNPPRRVVLLSSFFGGLLAAFFAAGGGGAAMMVVYYVVSSLLTTEEII 10 20 30 40 50 60 70 80 90 100 110 120 m901.pep FSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNPHETLDAQDPSFQESKRRH II II II II I II II I I II II II II II II II II II II I II II II I II II I I I II I I II I I I! a901 FSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNPHETLDAQDPSFQESKRRH 70 80 90 100 110 120 130 140 150 160 170 180 m901. pep IARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALAIAIHNIPEGISIAAPVYFAT I II II I II II II II II I II I II I II I II I I II II II II I I I II II II II I II I I II II I I a901 IARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALAIAIHNIPEGISIAAPVYFAT 130 140 150 160 170 180 190 200 210 220 230 240 m901. pep RSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFGSVFGVIAGVMVFLALDELXPAA I II II II I II I I II II I I II I I II II II I I I II I I I II II II II I I M I I I I M II III a901 RSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFGSVFGVIAGVMVFLALDELLPAA 190 200 210 220 230 240 250 260 270 m901.pep KRYSDGHETVYGLTTGMAVIAVSLVLFHFX II I I II II II II II I I II I II II I I II II a901 KRYSDGHETVYGLTMGMAVIAVSLVLFHFX 250 260 270 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2709>: g902.seq 1 ATGCCGTCCG AACCCGAACG GCGGCATGGC AATACTGCCC TACCCTTCCC 51 GATAGCCGCA CGCCCAACGG TCGGTTTTTC CGGCAAGCCT TTCAAGATAA 101 CCGGCAAGTG TGTCGTATTG CGCCGCCGCA TTGTCCAAGC GGTTGATTTC 151 ACGCCGCGCC TGTTCGCCGT CGGGCATTTC GCCGATGTAC CAGCCTATGT 201 GTTTGCGTGC GATGCGCACA CCGACGGTCT CACCATAAAA CGCGTGCATG 251 GCGCGGATGT GGTTCAAAAT GGCGGCTCTG CATTCTGCCA AACTCAAGGC 301 AGGCGGTAAA ACGCCGTGTT CGGCATAATG CTTCAAATCG CGGAAAAACC 351 ACGGCCTGCC TTGCGCGCCG CGCCCTATCA TGATGCCGTC GGCGGCGGTT 401 TGTTTGAGGA cggCGGCGGC TTTTTgcggc GAagtGATGT CGCCGTTGac 451 cCaggCCGGG ATGTTCAGAc ggCTTTTGGT CTCGGcgatg agttCGTAAC 501 gcGCCTCGCC TTTGTACATT TGCGTGcgcG CGcgcccgtg aacggcaaGg 551 gcggcaatgc cgcaatcttc ggcgattttg gcgacggcgG gcaggttttg 601 atcgtcgtcg tgccaaccca AacggGTTTT GaggGTAACG GGTAcgcCCG 651 CCGCCTTgac caccgcctcc aAAatggcGg caaccagcgg CTCGTCCTGC 701 ATCagcGCGC TACCGGCTTG GACGTTGCAC ACTTTCttgg cgggGCAGCC 751 CATAttgATG TCGATGACCT GCGCCCCGAG TCCGACGTTg taacgcgccg 801 catCCGCCAT CtgttcggGG TCGCTGCCGG CAATCTGCAC GGCAACGATG 851 CCGccttcat cggcaAAAtc actgcggtgc aGGGTTTTTC CGGTATTCCT 901 GAGCGTCGGA TCGCTGGCCA GCATTTCGCA CACCGCCCAA CCTGCGCCAA 951 ACGCCCGACA GAGGCGGCGG AAGGGTTTGT CGGCAATGCC CGCCATCGGC 1001 GCAAGTGCGA TGGGGTTGTC GATAAAATAA CCGCCGATGT GCATAATGGG 1051 CCCGCGTTTC AAAAAAGTGC GCCATTGTAC ATTTTTTAA This corresponds to the amino acid sequence <SEQ ID 2710; ORF 902.ng>: g902.pep 1 MPSEPERRHG ΝTALPFPIAA RPTVGFSGKP FKITGKCWL RRRIVQAVDF 51 TPRLFAVGHF ADVPAYVFAC DAHTDGLTIK RVHGAD QΝ GGSAFCQTQG 101 RR*ΝAVFGIM LQIAEKPRPA LRAAPYHDAV GGGLFEDGGG FLRRSDVAVD 151 PGRDVQTAFG LGDEFVTRLA FVHLRARAPV NGKGGNAAIF GDFGDGGQVL 201 IVWPTQTGF EGNGYARRLD HRLQNGGNQR LVLHQRATGL DVAHFLGGAA 251 HIDVDDLRPE SDWTRRIRH LFGVAAGNLH GNDAAFIGKI TAVQGFSGIP 301 ERRIAGQHFA HRPTCAKRPT EAAEGFVGNA RHRRKCDGW DKITADVHNG 351 PAFQKSAPLY IF* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2711>: m902.seq 1 TTGCACTTTC AAAGGATAAT CAAGTGTTCA GAAGGCATTT GGGCGGTAGG 51 CGCACGCCCA ACTGTCGGTT TTTTCGGCAA GTCTTTCAAG ATAACCTGCA 101 AGCATGTCGT ATTGCGCCGC CGCACTGTCC AAGCGGTTGA TTTCACGACG 151 TgTCTgTTCG CCGTcGGGCA TTTCGTCGAT GTACCAGCCT ATGTGTTTGC 201 GTGCGATGCG CACACCGGCG GTGTCGCCGT AAAACGCGTG TATGGCGCGG 251 ATGTGGTTCA AAATAGCGGC GGCGCATTCT GCCAAACTCA AGGCAGGCGG 301 CAAAACACCG TGTTCGGCAT AATGTTTCAA ATCGCGGAAG AACCACGGCC 351 TGCCTTGCGC GCCGCGCCCT ATCATAATGC CGTCGGCGGC GGTTTGTTTG 401 AGGACGGCTT GGGCTTTTTG CGGCGAAGTA ATGTCGCCGT TGACCCAGAC 451 CGGGATGTTC AGACGGCATT TGGTTTCGGC GATGAGTTCG TAACGCGCTT 501 CGCCTTTGTA CATTTGCGTA CGCGTGCGTC CGTGGACGGC AAGGGCGGCG 551 ATGCCGCAAT CTTCGGCGAT TTTGGCGATG ACGGGCAGGT TTTGATGGTC 601 GTCGTGCCAA CCCAAACGGG TTTTGAGGGT AACGGGTACG CCTGCCGCAC 651 GGACGACGGC TTCCAAAATG GCGGCAACCA GCGGCTCGTT CTGCATCAGC 701 GCGCTACCGG CTTGGACATT GCAGACTTTT TTAGCGGGAC AGCCCATGTT 751 GATGTCGATA AGCTGCGCCC CAAGGCTGAC GTTGTAACGC GCGGCATCCG 801 CCATCTGCTG CGGATCGCTT CCGGCAATCT GCACGGCAAC AATGCCGCCT 851 TCATCGGCAA AATCGCTGCG GTGCAAGGTT TTTCTAGTAT TTCTGAGCGT 901 CGGGTCGCTG GTCAGGATTT CGCACACCGC CCAACCTGCG CCAAAATCTC 951 GGCAAAGTCG GCGGAACGGT TTGTCGGTAA TGCCCGCCAT CGGcGCaAGT 1001 GCGATGGGGT TGTCGATAAA ATAGCCGCCG ATGTGCATAA TGGATCCGCG 1051 TTTCAAAAAA GTACGCCATT GTACATTTTT TAA This corresponds to the amino acid sequence <SEQ ID 2712; ORF 902>: m902.pep 1 LHFQRIIKCS EGIWAVGARP TVGFFGKSFK ITCKHWLRR RTVQAVDFTT 51 CLFAVGHFVD VPAYVFACDA HTGGVAVKRV YGAD QNSG GAFCQTQGRR 101 QNTVFGIMFQ IAEEPRPALR AAPYHNAVGG GLFEDGLGFL RRSNVAVDPD 151 RDVQTAFGFG DEFVTRFAFV HLRTRASVDG KGGDAAIFGD FGDDGQVLMV 201 WPTQTGFEG NGYACRTDDG FQNGGNQRLV LHQRATGLDI ADFFSGTAHV 251 DVDKLRPKAD WTRGIRHLL RIASGNLHGN NAAFIGKIAA VQGFSSISER 301 RVAGQHFAHR PTCAKISAKS AERFVGNARH RRKCDGWDK IAADVHNGSA 351 FQK5TPLYIF * Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 902 shows 80.9% identity over a 345 aa overlap with a predicted ORF (ORF 902.ng) from N gonorrhoeae: m902/g902 10 20 30 40 50 m902.pep LHFQRIIKCSEGIWAVGARPTVGFFGKSFKITCKHWLRRRTVQAVDFTTCLFAVGHF II llll I MMM MIIIM g902 MPSEPERRHGΝTALPFPIAARPTVGFSGKPFKITGKCWLRRRIVQAVDFTPRLFAVGHF 10 20 30 40 50 60 60 70 80 90 100 110 m902.pep VDVPAYVFACDAHTGGVAVKRVYGADWQΝSGGAFCQTQGRRQΝTVFGIMFQIAEEPRPA MMIMIMMM I = = = 11 h 11 II II h I = 1111 II II I hllllhlMMMM g902 ADVPAYVFACDAHTDGLTIKRVHGADWQΝGGSAFCQTQGRRXΝAVFGIMLQIAEKPRPA 70 80 90 100 110 120 120 130 140 150 160 170 m902. pep LRAAPYHΝAVGGGLFEDGLGFLRRSΝVAVDPDRDVQTAFGFGDEFVTRFAFVHLRTRASV IMIIIhlMMIMM I M 11 11111 IIMMMMIIIIIhlllllMM I g902 LRAAPYHDAVGGGLFEDGGGFLRRSDVAVDPGRDVQTAFGLGDEFVTRLAFVHLRARAPV 130 140 150 160 170 180 180 190 200 210 220 230 m902. pep DGKGGDAAI FGDFGDDGQVLMVWPTQTGFEGΝGYACRTDDGFQΝGGΝQRLVLHQRATGL : I ! II = 1111 [ 1111 IMhllMIMIMIMM I I MMMMMMMMM g902 ΝGKGGΝAAIFGDFGDGGQVLIVWPTQTGFEGΝGYARRLDHRLQΝGGΝQRLVLHQRATGL 190 200 210 220 230 240 240 250 260 270 280 290 m902.pep DIADFFSGTAHVDVDKLRPKADWTRGIRHLLRIASGΝLHGΝΝAAFIGKIAAVQGFSSIS Ml MMMMIII MhMIMI ! , A A II II M II II II h I II 11 h I g902 DVAHFLGGAAHIDVDDLRPESDWTRRIRHLFGVAAGΝLHGΝDAAFIGKITAVQGFSGIP 250 260 270 280 290 300 300 310 320 330 340 350 m902.pep E ERRRRV 1 AGQHFAHRPTCAKISAKSAERFVGΝARHRRKCDGWDKIAADVHΝGSAFQKSTPLY M1 111M11 MMMMIMM ■■■■ A\ II I II 11 II I II I II II M II 1111 Mllhlll g902 E ERRRRI:AGQHFAHRPTCAKRPTEAAEGFVGΝARHRRKCDGWDKITADVHΝGPAFQKSAPLY 310 320 330 340 350 360 360 m902.pep IFX 1 11111 g902 IFX The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2713>: a902. seq 1 TTGCACTTTC AAAGGATAAT CAAGTGTTCA GAAGGCATTT GGGCGGTAGG 51 CGCACGCCCA ACTGTCGGTT TTTTCGGCAA GTCTTTCAAG ATAACCTGCA 101 AACATGTCGT ATTGCGCCGC CGCACTGTCC AAGCGGTTGA TTTCACGACG 151 TGTCTGTTCG CCGTCGGGCA TTTCGTCGAT GTACCAGCCT ATGTGTTTGC 201 GTGCGATGCG CACACCGGCG GTGTCGCCGT AAAACGCGTG CATGGCTCGG 251 ATGTGGTTCA AAATAGTGGC GGTACATTCT GCCAAACTCA AGGCAGGCGG 301 TAAAACACCG TGTTCGGCGT AATGTTTCAA ATCGCGGAAG AACCACGGTC 351 TGCCTTGCGC GCCGCGCCCT ATCATAATGC CGTCTGCGGC GGTTTGTTTG 401 AGGACGGCTT GGGCTTTTTG CGGCGAGGTA ATGTCGCCGT TGACCCAGAC 451 CGGGATGTTC AGACGGCATT TGGTTTCGGC AATCAGGTCG TAAGCCGCTT 501 CGCCTTTGTA CATTTGCGTG CGCGTGCGTC CGTGGACGGC AAGGGCGGCA 551 ATGCCGCAAT CTTCGGCGAT TTTGGCGATG ACGGGCAGGT TTTGATGGTC 601 GTCGTGCCAA CCCAAACGGG TTTTGAGGGT AACGGGTACG CCCGCCGCTT 651 TGACCACCGC CTCCAAAATG GCGGCAACCA GCGGCTCGTT CTGCATCAGC 701 GCGCTACCGG CTTGGACATT GCAGACTTTT TTAGCGGGAC AGCCCATGTT -751 GATGTCGATA AGCTGCGCCC CAAGGCTGAC GTTGTAACGC GCGGCATCCG 801 CCATCTGCTG CGGATCGCTT CCGGCAATCT GCACGGCAAC AATGCCGCCT 851 TCATCGGCAA AATCGCTGCG GTGCAAGGTT TTTCTAGTAT TTCTGAGCGT 901 CGGGTCGCTG GTCAGCATTT CGCACACCGC CCAACCTGCG CCAAAATCTC 951 GGCAAAGTCG GCGGAACGGT TTGTCGGTAA TGCCCGCCAT CGGCGCAAGT 1001 GCGATGGGGT TGTCGATAAA ATAGCCGCCG ATGTGCATAA TGGATCCGCG 1051 TTTCAAAAAA GTACGCCATT GTACATTTTT TAA This corresponds to the amino acid sequence <SEQ ID 2714; ORF 902.a>: a902.pep 1 LHFQRIIKCS EGIWAVGARP TVGFFGKSFK ITCKHVVLRR RTVQAVDFTT 51 CLFAVGHFVD VPAYVFACDA HTGGVAVKRV HGSDVVQNSG GTFCQTQGRR 101 *NTVFGVMFQ IAEEPRSALR AAPYHNAVCG GLFEDGLGFL RRGNVAVDPD 151 RDVQTAFGFG NQWSRFAFV HLRARASVDG KGGNAAIFGD FGDDGQVLMV 201 VVPTQTGFEG NGYARRFDHR LQNGGNQRLV LHQRATGLDI ADFFSGTAHV 251 DVDKLRPKAD WTRGIRHLL RIASGNLHGN NAAFIGKIAA VQGFSSISER 301 RVAGQHFAHR PTCAKISAKS AERFVGNARH RRKCDGWDK IAADVHNGSA 351 FQKSTPLYIF * m902/a902 94.7% identity in 360 aa overlap 10 20 30 40 50 60 m902 . pep LHFQRIIKCSEGIWAVGARPTVGFFGKSFKITCKHWLRRRTVQAVDFTTCLFAVGHFVD M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M a902 LHFQRIIKCSEGIWAVGARPTVGFFGKSFKITCKHVVLRRRTVQAVDFTTCLFAVGHFVD 10 20 30 40 50 60 70 80 90 100 110 120 m902.pep VPAYVFACDAHTGGVAVKRVYGADWQNSGGAFCQTQGRRQNTVFGIMFQIAEEPRPALR II II II I I I I II I II I II I M M I I II I I II : II I II I II MMMMIMMM Ml a902 VPAYVFACDAHTGGVAVKRVHGSD QNSGGTFCQTQGRRXNTVFGVMFQIAEEPRSALR 70 80 90 100 110 120 130 140 150 160 170 180 m902. pep AAPYHNAVGGGLFEDGLGFLRRSNVAVDPDRDVQTAFGFGDEFVTRFAFVHLRTRASVDG II II II I I I II II II II II II: II I II I II II I II I II I :: I : I I II II I I : II I I II a902 AAPYHNAVCGGLFEDGLGFLRRGNVAVDPDRDVQTAFGFGNQVVSRFAFVHLRARASVDG 130 140 150 160 170 180 190 200 210 220 230 240 m902. pep KGGDAAIFGDFGDDGQVLMWVPTQTGFEGNGYACRTDDGFQNGGNQRLVLHQRATGLDI I I I : I II II II I II II II II I I II I II I II II I I I I : II II I II I I I II I II I I I I a902 KGGNAAIFGDFGDDGQVLMWVPTQTGFEGNGYARRFDHRLQNGGNQRLVLHQRATGLDI 190 200 210 220 230 240 250 260 270 280 290 300 m902.pep ADFFSGTAHVDVDKLRPKADVVTRGIRHLLRIASGNLHGNNAAFIGKIAAVQGFSSISER MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a902 ADFFSGTAHVDVDKLRPKADVVTRGIRHLLRIASGNLHGNNAAFIGKIAAVQGFSSISER 250 260 270 280 290 300 310 320 330 340 350 360 m902.pep RVAGQHFAHRPTCAKISAKSAERFVGNARHRRKCDGVVDKIAADVHNGSAFQKSTPLYIF a902 RVAGQHFAHRPTCAKISAKSAERFVGNARHRRKCDGWDKIAADVHNGSAFQKSTPLYIF 310 320 330 340 350 360 m902.pep X I a902 X The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2715>: g903.seq 1 ATGGCAACAC AGGTAGGCGG TGCAAattcG gatgaggCAA GCCCCTGCTT 51 TCCTATTTCT GAGGTGGAaT TGGTGGGTGA aGaaacggct aAATTCCGgt 101 tTGCGCTcaa ccaTGCCTTG tgccAAACAC ATTTTGtttc cGgcaagtgt 151 CTGcATGcgg gcgacatTAA TCAAAtcaTG TCCTTAGCAC AAAATGCTTT 201 GATCGGCAGG GGATATACCA CGACCCGTAT CTTGGCTGCG CCACAGGATT 251 TGAATAGTGG caaGCTTCAA TTAAccctga tgccggGCTA TCtgcgctcC 301 ATAcgaATCG atcggtccaa cgatgatcaa ACCCATgcAG GACGTATTGC 351 AGCATTCCAA AACAAATTTC CCACCCGCTC GAACGATCTG TTGAATCTGC 401 GTGATTTGGA ACAAGGACTG GAAAATCTCA AATGTCTCCC GACTGCGGAA 451 GCCGATCTCC AAATCgttcc cgtaGAGAGA GAACcAAACC AAAGTGATGT 501 CGTGGTGCAA TGGCGGTAAC GTCTGCTGCC CTACTGTGTG AGTGTGGGGA 551 TGGATAATTC GGGTAGTGAG GCGACAGGAA AATACCAAGG AAATATCACT 601 TTCTCTGCCG ACAATCCTTT TggactgAGT GATATGTTCT ATGTAAATTA 651 TGGACGTTCA ATTGGCGGTA CGcccgATGA GGAAAATTTT GACGGCCATC 701 GCAAAGAAGG CGGATCAAAC AATTACGCCG TACATTATTC AGCCCCTTTC 751 GGTAAATGGA CATGGGCATT CAATCACAAT GGCTACCGTT ACCATCAGGC 801 GGTTTCCGGA TTATCGGAAG TCTATGACTA TAATGGAAAA AGTTACAACA 851 CTGATTTCGG CTTCAACCGC CTGTTGTATC GTGATGCCAA ACGCAAAACC 901 TATCTCAGTG TAAAACTGTG GACGAGGGAA ACAAAAAGTT ACATTGATGA 951 TGCCGAACTG ACTGTACAAC GGCGTAAAAC CACAGGTTGG TTGGCAGAAC 1001 TTTCCCACAA AGGATATATC GGTCGCAGTA CGGCAGATTT TAAGTTGAAA 1051 TATAAACACG GCACCGGCAT GAAAGATGCT CTGCGCGCGC CTGAAGAAGC 1101 CTTTGGCGAA GGCACGTCAC GTATGAAAAT TTGGACGGCA TCGGCTGATG 1151 TAAATACTCC TTTTCAAATC GGTAAACAGC TATTTGCCTA TGACACATCC 1201 GTTCATGCAC AATGGAACAA AACCCCGCTA ACATCGCAAG ACAAACTGGC 1251 TATCGGCGGA CACCACACCG TACGTGGCTT CGACGGTGAA ATGAGTTTGC 1301 CTGCCGAGCG GGGATGGTAT TGGCGCAACG ATTTGAGCTG GCAATTTAAA 1351 CCAGGCCATC AGCTTTATCT TGGGGCTGAT GTAGGACATG TTTCAGGACA 1401 ATCCGCCAAA TGGTTATCGG GCCAAACTCT AGCCGGCACA GCAATTGGGA 1451 TACGCGGGCA GATAAAGCTT GGCGGCAACC TGCATTACGA TATATTTACC 1501 GGCCGTGCAT TGAAAAAGCC cgaatatttt cAGACGAAGA Aatgggtaac 1551 ggggtTTCAG gtgggttatt cgTTTTGA This corresponds to the amino acid sequence <SEQ ID 2716; ORF 903.ng>: g903.pep 1 MATQVGGAΝS DEASPCFPIS EVELVGEETA KFRFALΝHAL CQTHFVSGKC 51 LHAGDIΝQIM SLAQΝALIGR GYTTTRILAA PQDLΝSGKLQ LTLMPGYLRS 101 IRIDRSΝDDQ THAGRIAAFQ ΝKFPTRSΝDL LΝLRDLEQGL EΝLKCLPTAE 151 ADLQIVPVER EPΝQSDVWQ WRXRLLPYCV SVGMDΝSGSE ATGKYQGΝIT 201 FSADΝPFGLS DMFYVΝYGRS IGGTPDEEΝF DGHRKEGGSΝ ΝYAVHYSAPF 251 GKWTWAFΝHΝ GYRYHQAVSG LSEVYDYΝGK SYΝTDFGFΝR LLYRDAKRKT 301 YLSVKLWTRE TKSYIDDAEL TVQRRKTTGW LAELSHKGYI GRSTADFKLK 351 YKHGTGMKDA LRAPEEAFGE GTSRMKIWTA SADVΝTPFQI GKQLFAYDTS 401 VHAQWΝKTPL TSQDKLAIGG HHTVRGFDGE MSLPAERGWY WRΝDLSWQFK 451 PGHQLYLGAD VGHVSGQSAK WLSGQTLAGT AIGIRGQIKL GGΝLHYDIFT 501 GRALKKPEYF QTKKWVTGFQ VGYSF* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2717>: m903.seq 1 ATGCAGCGTC AGCAGCACAT AGATGCTGAA TTGTTAACTG ATGCAAATGT 51 CCGTTTCGAG CAACCATTGG AGAAGAACAA TTATGTCCTG AGTGAAGATG 101 AAACACCGTG TACTCGGGTA AATTACATTA GTTTAGATGA TAAGACGGTG 151 CGCAAATTTT CTTTTCTTCC TTCTGTGCTC ATGAAAGAAA CAGCTTTTAA 201 AACTGGGATG TGTTTAGGTT CCAATAATTT GAGCAGGCTA CAAAAAGCCG 251 CGCAACAGAT ACTGATCGTG CGTGGCTACC TCACTTCCCA AGCTATTATC 301 CAaCCACAGA ATATGGATTC GGGAATTCTG AAATTACGGG TATCAGCAGG 351 CGAAATAGGG GATATCCGCT ATGAAGAAAA ACGGGATGGG AAGTCTGCCG 401 AGGGCAGTAT TAGTGCATTC AATAACAAAT TTCCCTTATA TAGGAACAAA 451 ATTCTCAATC TTCGCGATGT AGAGCAGGGC TTGGAAAACC TGCGTCGTTT 501 GCCGAGTGTT AAAACAGATA TTCAGATTAT ACCGTCCGAA GAAGAAGGCA 551 AAAGCGATTT ACAGATCAAA TGGCAGCAGA ATAAACCCAT ACGGTTCAGT 601 ATCGGTATAG ATGATGCGGG CGGCAAAACG ACCGGCAAAT ATCAAGGAAA 651 TGTCGCTTTA TCGTTCGATA ACCCTTTGGG CTTAAGCGAT TTGTTtTATG 701 TTTCATATGG ACGCGGTTTG GCGCACAAAA CGGACTTGAC TGATGCCACC 751 GGTACGGAAA CTGAAAGCGG ATCCAGAAGT TACAGCGTGC ATTATTCGGT 801 GCCCGTAAAA AAATGGCTGT TTTCTTTTAA TCACAATGGA CATCGTTACC 851 ACGAAGCAAC CGAAGGCTAT TCCGTCAATT ACGATTACAA CGGCAAACAA 901 TATCAGAGCA GCCTGGCCGC CGAGCGCATG CTTTGGCGTA ACAGACTTCA 951 TAAAACTTCA GTCGGAATGA AATTATGGAC ACGCCAAACC TATAAATACA 1001 TCGACGATGC CGAAATCGAA GTACAACGCC GCCGCTCTGC AGGCTGGGAA 1051 GCCGAATTGC GCCACCGTGC TTACCTCAAC CGTTGGCAGC TTGACGGCAA 1101 GTTGTCTTAC AAACGCGGGA CCGGCATGCG CCAAAGTATG CCTGCACCGG 1151 AAGAAAACGG CGGCGATATT CTTCCAGGTA CATCTCGTAT GAAAATCATT 1201 ACTGCCAGTT TGGACGCAGC CGCCCCATTT AyTTTAGGCA AACAGCAGTT 1251 TTTCTACGCA ACCGCCATTC AAGCTCAATG GAACAAAACG CCGTTGGTTG 1301 CCCAAGATAA ATTGTCAATC GGCAGCCGCT ACACCGTTCG CGGATTTGAT 1351 GGGGAGCAGA GTCTTTTCGG AGAGCGAGGT TTCTACTGGC AGAATACTTT 1401 AACTTGGTAT TTTCATCCGA ACCATCAGTT CTATCTCGGT GCGGACTATG 1451 GCCGCGTATC TGGCGAAAGT GCACAATATG TATCGGGCAA GCAGCTGATG 1501 GGTGCAGTGG TCGGCTTCAG AGGAGGGCAT AAAGTAGGCG GTATGTTTGC 1551 TTATGATCTG TTTGCCGGCA AGCCGCTTCA TAAACCCAAA GGCTTTCAGA 1601 CGACCAACAC CGTTTACGGC TTCAACTTGA ATTACAGTTT CTAA This corresponds to the amino acid sequence <SEQ ID 2718; ORF 903>: m903.pep 1 MQRQQHIDAE LLTDANVRFE QPLEKNNYVL SEDETPCTRV NYISLDDKTV 51 RKFSFLPSVL MKETAFKTGM CLGSNNLSRL QKAAQQILIV RGYLTSQAII 101 QPQNMDSGIL KLRVSAGEIG DIRYEEKRDG KSAEGSISAF NNKFPLYRNK 151 ILNLRDVEQG LENLRRLPSV KTDIQIIPSE EEGKSDLQIK WQQNKPIRFS 201 IGIDDAGGKT TGKYQGNVAL SFDNPLGLSD LFYVSYGRGL AHKTDLTDAT 251 GTETESGSRS YSVHYSVPVK KWLFSFNHNG HRYHEATEGY SVNYDYNGKQ 301 YQSSLAAERM LWRNRLHKTS VGMKLWTRQT YKYIDDAEIE VQRRRSAGWE 351 AELRHRAYLN RWQLDGKLSY KRGTGMRQSM PAPEENGGDI LPGTSRMKII 401 TASLDAAAPF XLGKQQFFYA TAIQAQWNKT PLVAQDKLSI GSRYTVRGFD 451 GEQSLFGERG FYWQNTLTWY FHPNHQFYLG ADYGRVSGES AQYVSGKQLM 501 GAWGFRGGH KVGGMFAYDL FAGKPLHKPK GFQTTNTVYG FNLNYSF* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 903 shows 48.9% identity over a 519 aa overlap with a predicted ORF (ORF 903.ng) from N gonorrhoeae: m903/g903 10 20 30 40 50 60 m903.pep MQRQQHIDAELLTDAΝVRFEQPLEKΝΝYVLSEDETPCTRVΝYISLDDKTVRKFSFLPSVL |:: :|| :: : | : : || | : g903 MATQVGGAΝSDEASPCFPISEVELVGEETAKFRFALΝHA 10 20 30 70 80 90 100 110 120 m903.pep MKETAFKTGMCLGSΝΝLSRLQKAAQQILIVRGYLTSQAIIQPQΝMDSGILKLRVSAGEIG ■ ■ A \ A II :: = : ::::: ||: || ||| = : I I = = = I I hi = I : g903 LCQTHFVSGKCLHAGDIΝQIMS AQΝALIGRGYTTTRILAAPQDLΝSGKLQLTLMPGYLR 40 50 60 70 80 90 130 140 150 160 170 180 m903. pep DIRYEEKRDGKSAEGSISAFNNKFPLYRNKILNLRDVEQGLENLRRLPSVKTDIQIIPSE Ml = : = I = = I IMMIIII I MMIIMMMIh I f = = = = I = 11 = I I g903 SIRIDRSNDDQTHAGRIAAFQNKFPTRSNDLLNLRDLEQGLENLKCLPTAEADLQIVPVE 100 110 120 130 140 150 190 200 210 220 230 m903.pep EE-GKSDLQIKWQQNK-PIRFSIGIDDAGGKTTGKYQGNVALSFDNPLGLSDLFYVSYGR = 1 -I = = h I hhh = h = = IMMM = = = l MMIMMMMIM g903 REPNQSDVWQWRXRLLPYCVSVGMDNSGSEATGKYQGNITFSADNPFGLSDMFYVNYGR 160 170 180 190 200 210 240 250 260 270 280 290 m903.pep GLAHKTDLTDATGTETESGSRSYSVHYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNG = = = 1 = 1 = hll =hlllhl II = = lllll = llhh I I Mill g903 SIGGTPDEENFDGHRKEGGSNNYAVHYSAPFGKWTWAFNHNGYRYHQAVSGLSEVYDYNG 220 230 240 250 260 270 300 310 320 330 340 350 m903.pep KQYQSSLAAERMLWRNRLHKTSVGMKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAY |:|::::: :|:h|: :|| :::|||||:| Mllllh lllh = = ll III h = l g903 KSYNTDFGFNRLLYRDAKRKTYLSVKLWTRETKSYIDDAELTVQRRKTTGWLAELSHKGY 280 290 300 310 320 330 360 370 380 390 400 410 m903. pep LNRWQLDGKLSYKRGTGMRQSMPAPEENGGDILPGTSRMKIITASLDAAAPFXLGKQQFF = = l I lhlhllll = = = = llll 1= III h =11 =111 I g903 IGRSTADFKLKYKHGTGMKDALRAPEEAFGE GTSRMKIWTASADVNTPFQIGKQLFA 340 350 360 370 380 390 420 430 440 450 460 470 m903. pep YATAIQAQWNKTPLVAQDKLSIGSRYTVRGFDGEQSLFGERGFYWQNTLTWYFHPNHQFY I l = = = = lllhll = : = =111 = 11 = 1 hi hhlhl g903 YDTSVHAQWNKTPLTSQDKLAIGGHHTVRGFDGEMSLPAERGWYWRNDLSWQFKPGHQLY 400 410 420 430 440 450 480 490 500 510 520 530 m903. pep LGADYGRVSGESAQYVSGKQLMGAWGFRGGHKVGGMFAYDLFAGKPLHKPKGFQTTNTV llll |:|||:||:::||: | h-hll hll = Ihhh 1 = 11= III = I g903 LGADVGHVSGQSAKWLSGQTLAGTAIGIRGQIKLGGNLHYDIFTGRALKKPEYFQTKKWV 460 470 480 490 500 510 540 m903.pep YGFNLNYSFX I III • • • • - • 11 IIII 11 g903 TGFQVGYSFX 520 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2719>: a903.seq 1 ATGCAGCGTC AGCAGCACAT AGATGCTGAA TTGTTAACTG ATGCAAATGT 51 CCGTTTCGAG CAACCATTGG AGAAGAACAA TTATGTCCTG AGTGAAGATG 101 AAACACCGTG TACTCGGGTA AATTACATTA GTTTAGATGA TAAGACGGCG 151 CGCAAATTTT CTTTTCTTCC TTCTGTGCTC ATGAAAGAAA CAGCTTTTAA 201 AACTGGGATG TGTTTAGGTT CCAATAATTT GAGCAGGCTA CAAAAAGCCG 251 CGCAACAGAT ACTGATTGTG CGTGGCTACC TCACTTCCCA AGCTATTATC 301 CAACCACAGA ATATGGATTC GGGAATTCTG AAATTACGGG TATCAGCAGG 351 CGAAATAGGG GATATCCGCT ATGAAGAAAA ACGGGATGGG AAGTCTGCCG 401 AGGGCAGTAT TAGTGCATTC AATAACAAAT TTCCCTTATA TAGGAACAAA 451 ATTCTCAATC TTCGCGATGT AGAGCAGGGC TTGGAAAACC TGCGTCGTTT 501 GCCGAGTGTT AAAACAGATA TTCAGATTAT ACCGTCCGAA GAAGAAGGCA 551 AAAGCGATTT ACAGATCAAA TGGCAGCAGA ATAAACCCAT ACGGTTCAGT 601 ATCGGTATAG ATGATGCGGG CGGCAAAACG ACCGGCAAAT ATCAAGGAAA 651 TGTCGCTTTA TCGTTCGATA ACCCTTTGGG CTTAAGCGAT TTGTTTTATG 701 TTTCATATGG ACGCGGTTTG GTGCACAAAA CGGACTTGAC TGATGCCACC 751 GGTACGGAAA CTGAAAGCGG ATCCAGAAGT TACAGCGTGC ATTATTCGGT 801 GCCCGTAAAA AAATGGCTGT TTTCTTTTAA TCACAATGGA CATCGTTACC 851 ACGAAGCAAC CGAAGGCTAT TCCGTCAATT ACGATTACAA CGGCAAACAA 901 TATCAGAGCA GCCTGGCCGC CGAGCGCATG CTTTGGCGTA ACAGGTTTCA 951 TAAAACTTCA GTCGGAATGA AATTATGGAC ACGCCAAACC TATAAATACA 1001 TCGACGATGC CGAAATCGAA GTGCAACGCC GCCGCTCTGC AGGCTGGGAA 1051 GCCGAATTGC GCCACCGTGC TTACCTCAAC CGTTGGCAGC TTGACGGCAA 1101 GTTGTCTTAC AAACGCGGGA CCGGCATGCG CCAAAGTATG CCCGCACCTG 1151 AAGAAAACGG CGGCGGTACT ATTCCAGGCA CATCCCGTAT GAAAATCATA 1201 ACCGCCGGAT TGGATGCAGC GGCCCCGTTT ATGTTGGGCA AACAGCAGTT 1251 TTTCTACGCA ACCGCCATTC AAGCTCAATG GAACAAAACG CCTTTGGTTG 1301 CCCAAGACAA GTTGTCTATC GGCAGCCGCT ACACCGTTNG CGGATTTGAT 1351 GGGGAGCAGA GTCTTTTCGG AGAGCGAGGT TTCTACTGGC AGAATACTTT 1401 AACTTGGTAT TTTCATCCGA ACCATCAGTT CTATCTCGGT GCGGACTATG 1451 GCCGCGTATC TGGCGAAAGT GCACAATATG TATCGGGCAA GCAGCTGATG 1501 GGTGCAGTGG TCGGNTTCAG AGGAGGNCAT AAAGTAGGCG GTATGTTTGC 1551 TTATGATCTG TTTGCCGGCA AGCCGCTTCA TAAACCCAAA GGCTTTCAGA 1601 CGACCAACAC CGTTTACGGC TTCAACTTGA ATTACAGTTT CTAA This corresponds to the amino acid sequence <SEQ ID 2720; ORF 903. a>: a903.pep 1 MQRQQHIDAE LLTDANVRFE QPLEKNNYVL SEDETPCTRV NYISLDDKTA 51 RKFSFLPSVL MKETAFKTGM CLGSNNLSRL QKAAQQILIV RGYLTSQAII 101 QPQNMDSGIL KLRVSAGEIG DIRYEEKRDG KSAEGSISAF NNKFPLYRNK 151 ILNLRDVEQG LENLRRLPSV KTDIQIIPSE EEGKSDLQIK WQQNKPIRFS 201 IGIDDAGGKT TGKYQGNVAL SFDNPLGLSD LFYVSYGRGL VHKTDLTDAT 251 GTETESGSRS YSVHYSVPVK KWLFSFNHNG HRYHEATEGY SVNYDYNGKQ 301 YQSSLAAERM LWRNRFHKTS VGMKLWTRQT YKYIDDAEIE VQRRRSAGWE 351 AELRHRAYLN RWQLDGKLSY KRGTGMRQSM PAPEENGGGT IPGTSRMKII 401 TAGLDAAAPF MLGKQQFFYA TAIQAQWNKT PLVAQDKLSI GSRYTVXGFD 451 GEQSLFGERG FYWQNTLTWY FHPNHQFYLG ADYGRVSGES AQYVSGKQLM 501 GAWGFRGGH KVGGMFAYDL FAGKPLHKPK GFQTTNTVYG FNLNYSF* m903/a903 98.4% identity in 547 aa overlap 10 20 30 40 50 60 m903. pep MQRQQHIDAELLTDANVRFEQPLEKNNYVLSEDETPCTRVNYISLDDKTVRKFSFLPSVL I I II I II II II I II II I II I II II II I I II II II II II II I I I I I I I I I : I I II I I I I I I a903 MQRQQHIDAELLTDANVRFEQPLEKNNYVLSEDETPCTRVNYISLDDKTARKFSFLPSVL 10 20 30 40 50 60 70 80 90 100 110 120 m903.pep MKETAFKTGMCLGSNNLSRLQKAAQQILIVRGYLTSQAIIQPQNMDSGILKLRVSAGEIG II II II II I II II II I II II II I II II I I II I I II II II I II II I I II I II II I I I I II I a903 MKETAFKTGMCLGSNNLSRLQKAAQQILIVRGYLTSQAIIQPQNMDSGILKLRVSAGEIG 70 80 90 100 110 120 130 140 150 160 170 180 m903. pep DIRYEEKRDGKSAEGSISAFNNKFPLYRNKILNLRDVEQGLENLRRLPSVKTDIQIIPSE II II I II II II II II II I I II I I I I II I II II II II I II II II II II I I II I II II II II a903 DIRYEEKRDGKSAEGSISAFNNKFPLYRNKILNLRDVEQGLENLRRLPSVKTDIQIIPSE 130 140 150 160 170 180 190 200 210 220 230 240 m903.pep EEGKSDLQIKWQQNKPIRFSIGIDDAGGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGL II II II II II II II I I II II I II II II I II II I I II II I I II I I II II I I I II I I I II II a903 EEGKSDLQIKWQQNKPIRFSIGIDDAGGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGL 190 200 210 220 230 240 250 260 270 280 290 300 m903.pep AHKTDLTDATGTETESGSRSYSVHYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQ : M I II I II II I I I I II II I II I II I I I I II II II II II I II II I I I I I I I I I I I I I I I I a903 VHKTDLTDATGTETESGSRSYSVHYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQ 250 260 270 280 290 300 310 320 330 340 350 360 m903. pep YQSSLAAERMLWRNRLHKTSVGMKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAYLN MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a903 YQSSLAAERMLWRNRFHKTSVGMKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAYLN 310 320 330 340 350 360 370 380 390 400 410 420 m903. pep RWQLDGKLSYKRGTGMRQSMPAPEENGGDILPGTSRMKIITASLDAAAPFXLGKQQFFYA II II II II II I II II II II I II II I II I : I II II II I I I I : I II II II I I II II II I a903 RWQLDGKLSYKRGTGMRQSMPAPEENGGGTIPGTSRMKIITAGLDAAAPFMLGKQQFFYA 370 380 390 400 410 420 430 440 450 460 470 480 m903.pep TAIQAQWNKTPLVAQDKLSIGSRYTVRGFDGEQSLFGERGFYWQNTLTWYFHPNHQFYLG II II II II II I I II II I II II II I II I II II I II I I II II II II II I I II I I I II II II a903 TAIQAQWNKTPLVAQDKLSIGSRYTVXGFDGEQSLFGERGFYWQNTLTWYFHPNHQFYLG 430 440 450 460 470 480 490 500 510 520 530 540 m903. pep ADYGRVSGESAQYVSGKQLMGAWGFRGGHKVGGMFAYDLFAGKPLHKPKGFQTTNTVYG MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a903 ADYGRVSGESAQYVSGKQLMGAWGFRGGHKVGGMFAYDLFAGKPLHKPKGFQTTNTVYG 490 500 510 520 530 540 m903.pep FNLNYSFX II II II II a903 FNLNYSFX The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2721>: g904. seq 1 ATGATGCAGC ACAATCGTTT CTTCGCGGTC GGGGCCGGTg gaGACGATGG 51 CGACCGGCGC GCCGCAGACT TCTTCAATCC GTTTCAAATA TGCTTTGGCA 101 TTGGCAGGCA ATGCGTCGTA GCTTTTCACG CCGACAGTCG ATTCGCGCCA 151 GCCGGGCATG GTTTCGTAAA TCGGTTTGCA GGTTTCCACC GCATCCGAAC 201 CGCAAGGCAG GATGTCGGTT TTGCCGCCGC CTGGCAATTC GTAGCCGACG 251 CAGATATTGA TGGTTTCAAC GCCGTCCATT ACATCGAGTT TGGTAATGCA 301 CATACCGGAA ATGCCGTTGA TTTGGATGGA GCGTTTCAGG GCGGCGGCAT 351 CAAACCAGCC GCAGCGGCGC GCGCGGCCGG TTACCGAACC GAATTCGTGT 401 CCGCGCTCCG CCAAACCTGC GCCTACTTCG TCGAACAATT CGGTCGGGAA 451 CGGGCCCGAA CCGACGCGCG TGGTATAGGC TTTGACGATG CCCAAAACAT 501 AATCCAGCAT TTGAGGACCT ACGCCCGCGC CTGCCGAAGC CGCGCCGGCG 551 AGACAGTTGG ACGAGGTAAC GAAGGGGTAA GTGCCGTAGT CGATGTCCAA 601 CAACGCACCT TGCGCGCCTT CAAACAGCAG TTTTTCGCCG TTTTTGTTTT 651 TTTCGTTCAA CACGCgggaC acgtcgGCAA TCATCGGCGC AATGCGCGGC 701 GCGACTTTTT CGATAACCGC CATCACGTCT TCCGCTTTAA CCGGTCCGGC 751 GTTATGCAGG TATTGGAGTT GGACGTTGTA ATAGGCAAGG ACGGCATCCA 801 GTTTTTCACG CAGTTTTTCA GGATGCAGCA AATCGGCGGC GCGAATGGCG 851 CGGCGTGCCA CTTTGTCTTC GTAGGCAGGG CCGATGCCGC GGCCGGTCGT 901 GCCGATTTTG CCTTTGCCGC GCGATGCTTC GCGGGCTTGG TCGAGCGCGA 951 TGTGGTAAGG CAGGATCAGC GGGCAGGTCG GCGCGATTTT CAGACGGCCT 1001 TCGACGTTTT TCACGCCTGC CGCGTTCAAC TCGTCGATTT CGCCCAACAG 1051 GGCTTCGGGg gaaacgAcaa cGCCCGAACC gatGAAGCAA TCCAATCCTT 1101 CGTGCAGGAT ACCGCTCGGA ATCAGGCGCA AAATGGTTTT TTTGCCGCCG 1151 ACGACCAAGG TATGGCCCGC ATTGTGGCCG CCTTGGAAGC GCACgacGct 1201 gCCGGCTTCT TCCGCCAGCC AGTCAACGAT TTTACCTTTA CCCTCGTCGC 1251 CCCACTGTgc gccGATTACT ACAACATTTT TAGCCATAGC CATATAACCT 1301 ATCGatatTA A This corresponds to the amino acid sequence <SEQ ID 2722; ORF 904.ng>: g904.pep 1 MMQHΝRFFAV GAGGDDGDRR AADFFΝPFQI CFGIGRQCW AFHADSRFAP 51 AGHGFVNRFA GFHRIRTARQ DVGFAAAWQF VADADIDGFN AVHYIEFGNA 101 HTGNAVDLDG AFQGGGIKPA AAARAAGYRT EFVSALRQTC AYFVEQFGRE 151 RARTDARGIG FDDAQNIIQH LRTYARACRS RAGETVGRGN EGVSAWDVQ 201 QRTLRAFKQQ FFAVFVFFVQ HAGHVGNHRR NARRDFFDNR HHVFRFNRSG 251 VMQVLELDW IGKDGIQFFT QFFRMQQIGG ANGAACHFVF VGRADAAAGR 301 ADFAFAARCF AGLVERDWR QDQRAGRRDF QTAFDVFHAC RVQLVDFAQQ 351 GFGGNDNART DEAIQSFVQD TARNQAQNGF FAADDQGMAR IVAALEAHDA 401 AGFFRQPVND FTFTLVAPLC ADYYNIFSHS HITYRY* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2723>: m904. seq 1 ATGATGCAGC ACAATCGTTT CTTCTCGGTC GGGGCCGgTG GAGACGATGG 51 CGACCGGCGC GCCGCAGACT TCTTCAATCC GTTTCAAATA TGCTTTGGCG 101 TTTTCGGGCA ATGCGCCGTA GTCCTTCACG CCGAAAGTGG ATTCGCGCCA 151 GCCGGGCATG GTTTCGTAAA TCGGCTTGCA GGTTTCCACC GCATCGGAAC 201 CGCAAGGCAG GATGTCGGTT TTGCCGCCGT CGGGCAATTC ATAGCCGACG 251 CAGATATTGA TGGTTTCAAC GCCGTCCATT ACATCGAGTT TAGTAATACA 301 CATACCGGAA ATGCCGTTGA TTTGGATGGA GCGTTTCAGG GCGGCGGCAT 351 CAAACCAGCC GCAGCGGCGT GCGCGTCCGG TTACCGAACC GAATTCGTGT 401 CCGCGTTCTG CCAAACCTAC GCCTACTTCG TCGAACAATT CGGTCGGGAA 451 CGGGCCCGAA CCGACGCGCG TGGTATAGGC TTTGACGATG CCCAAAACAT 501 AATCCAGCAT TTGAGGACCT ACGCCCGCGC CTGCCGAAGC TGCGCCCGCC 551 AGACAGTTGG ACGAGGTAAC GAAGGGATAA GTGCCGTAGT CGATGTCCAA 601 CAACGCACCT TGCGCGCCTT CAAACAGCAG TTTTTCGCCG TTTTTGTTTT 651 TCTCGTTCAA CACGCGGGAC ACGTCGGTAA TCATCGGCGC AATGCGCGGC 701 GCGACTTTTT CGATAACCGC CATCACGTCT TCCGCTTTAA CCGGCTCGGC 751 ATTGTGCAGA TGTTGCAGTT GGACATTGTA ATAGGCAAGG ACGGCATCCA 801 GTTTTTCACG CAGTTTyTCA GGATGCAGCA AATCGGCGGC GCGAATGGCG 851 CGGCGTGCCA CTTTGTCTTC GTAGGCAGGG CCGATGCCGC GGCCGGTCGT 901 GCCGATTTTG CCTTTGCCGC GCG.ATcTTC GCGGGCTTGG TCGAGCGCGA 951 TGTGGTAAGG CAGGATCAGC GGGCAGGTCG GCGCGATTTT CAGACGGCCT 1001 TCGACGTTTT TCACGCCTGC CGCGTTCAAC TCGTCGATTT CGCCCAACAG 1051 GGCTTCGGGG GAGACGACAA CGCCCGAACC GATGAAGCAG TCCAAACTTT 1101 CATGCAGGAT GCCGCTCGGA ATCAGGCGCA AAATGGTTTT TTTGCCGCCG 1151 ACAACCAAGG TATGGCCCGC ATTGTGGCCG CCTTGGAAGC GCACCaCGCC 1201 GCCGGCTTCT TCCGCCAGCC AGTCAACGAT TTTACCTTTA CCCTCGTCGC 1251 CCCACTGTGC GCCGATTAsT ACAACATTTT TAGCCATAGC CATATAACCT 1301 ATCGATATTA A This corresponds to the amino acid sequence <SEQ ID 2724; ORF 904>: m904 . pep 1 MMQHΝRFFSV GAGGDDGDRR AADFFΝPFQI CFGVFGQCAV VLHAESGFAP 51 AGHGFVΝRLA GFHRIGTARQ DVGFAAVGQF IADADIDGFΝ AVHYIEFSΝT 101 HTGNAVDLDG AFQGGGIKPA AAACASGYRT EFVSAFCQTY AYFVEQFGRE 151 RARTDARGIG FDDAQNIIQH LRTYARACRS CARQTVGRGN EGISAWDVQ 201 QRTLRAFKQQ FFAVFVFLVQ HAGHVGNHRR NARRDFFDNR HHVFRFNRLG 251 IVQMLQLDIV IGKDGIQFFT QFXRMQQIGG ANGAACHFVF VGRADAAAGR 301 ADFAFAAXIF AGLVERDWR QDQRAGRRDF QTAFDVFHAC RVQLVDFAQQ 351 GFGGDDNART DEAVQTFMQD AARNQAQNGF FAADNQGMAR IVAALEAHHA 401 AGFFRQPVND FTFTLVAPLC ADXYNIFSHS HITYRY* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 904 shows 90.4% identity over a 436 aa overlap with a predicted ORF (ORF 904.ng) from N gonorrhoeae: m904/g904 10 20 30 40 50 60 m904.pep MMQHΝRFFSVGAGGDDGDRRAADFFΝPFQICFGVFGQCAWLHAESGFAPAGHGFVΝRLA 111111 M = 11 II M 11111 M 111111111 M = lhh = lhl I M 111 II 111 = I g904 MMQHΝRFFAVGAGGDDGDRRAADFFΝPFQICFGIGRQCWAFHADSRFAPAGHGFVΝRFA 10 20 30 40 50 60 70 80 90 100 110 120 m904. pep GFHRIGTARQDVGFAAVGQFIADADIDGFNAVHYIEFSNTHTGNAVDLDGAFQGGGIKPA Mill IIMMMIh II = II II I II II 11 II II I = h II II II II I II I II II II II g904 GFHRIRTARQDVGFAAAWQFVADADIDGFNAVHYIEFGNAHTGNAVDLDGAFQGGGIKPA 70 80 90 100 110 120 130 140 150 160 170 180 m904.pep AAACASGYRTEFVSAFCQTYAYFVEQFGRERARTDARGIGFDDAQNIIQHLRTYARACRS Ml hMIMMIh II II II II II II I II II II I II II II II II I II II I II II II g904 AAARAAGYRTEFVSALRQTCAYFVEQFGRERARTDARGIGFDDAQNIIQHLRTYARACRS 130 140 150 160 170 180 190 200 210 220 230 240 m904. pep CARQTVGRGNEGI SAWDVQQRTLRAFKQQFFAVFVFLVQHAGHVGNHRRNARRDFFDNR I g904 RAGETVGRGNEGVSAWDVQQRTLRAFKQQFFAVFVFFVQHAGHVGNHRRNARRDFFDNR 190 200 210 220 230 240 250 260 270 280 290 300 m904.pep HHVFRFNRLGIVQMLQLDIVIGKDGIQFFTQFXRMQQIGGANGAACHFVFVGRADAAAGR M M! h = hhlhlMMMMMM I II II II I II II II II II I II II I II I g904 HHVFRFNRSGVMQVLELDWIGKDGIQFFTQFFRMQQIGGANGAACHFVFVGRADAAAGR 250 260 270 280 290 300 310 320 330 340 350 360 m904.pep ADFAFAARIFAGLVERDWRQDQRAGRRDFQTAFDVFHACRVQLVDFAQQGFGGDDNART 11111111111111111 II 11111 II I II 1111111 II 11111 M = 111 M g904 ADFAFAARCFAGLVERDWRQDQRAGRRDFQTAFDVFHACRVQLVDFAQQGFGGNDNART 310 320 330 340 350 360 370 380 390 400 410 420 m904. pep DEAVQTFMQDAARNQAQNGFFAADNQGMARIVAALEAHHAAGFFRQPVNDFTFTLVAPLC MhhhlhMMMMMMhMIMMMMM 1111 II II II I II I II II II I g904 DEAIQSFVQDTARNQAQNGFFAADDQGMARIVAALEAHDAAGFFRQPVNDFTFTLVAPLC 370 380 390 400 410 420 430 m904.pep ADXYNIFSHSHITYRYX I MI 1II11I 1I 1I 1I 1II11I III1 IMI 11 g904 ADYYNIFSHSHITYRYX 430 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2725>: a90 .seq 1 ATGATGCAGC ACAATCGTTT CTTCGCGGTC GGGGCCGGTG GAGACGATGG 51 CGACCGGCGC ACCGCAGACT TCTTCAATCC GTTTCAAATA TGCTTTGGCA 101 TTGGCAGGTA ATGCGTCGTA GCTTTTCACG CCGAAAGTGG ATTCGCTCCA 151 ACCGGGCATG GTTTCGTAAA TCGGCTTGCA GGCTTCTACC GCATCAGAGC 201 CGCAAGGCAG GATGTCGGTT TTGCCGCCGT CGGGCAATTC GTAGCCGACG 251 CAGATATTGA TGGTTTCAAC GCCGTCCATT ACATCGAGTT TGGTAATACA 301 CATACCGGAA ATGCCGTTGA TTTGGATGGA GCGTTTCAGG GCGGCGGCAT 351 CAAACCAGCC GCAGCGGCGT GCGCGTCCGG TTACCGAACC GAATTCGTGT 401 CCGCGTTCTG CCAAACCTGC TCCGACTTCG TCGAACAATT CGGTCGGGAA 451 CGGGCCCGAA CCGACGCGCG TGGTATAGGC TTTGACGATG CCCAAAACAT 501 AATCCAGCAT TTGAGGGCCT ACGCCCGCGC CTGCCGAAGC CGCGCCGGCG 551 AGGCAGTTGG ACGAAGTAAC GAAGGGGTAA GTGCCGTAGT CGATGTCCAA 601 CAACGCACCT TGCGCGCCTT CAAACAGCAG TTTTTCGCCG TTTTTGTTTT 651 TTTCGTTCAA CACGCGGGAC ACGTCGGTAA TCATCGGCGT AATGCGCGGC 701 GCGACTTTTT CGATAACCGC CATCACGTCT TCCGCTTTCA CCGACTCGGC 751 ATTGTGCAGA TGTTGCAGTT GGACGTTGTA ATAAGCAAAG ACGGCATCCA 801 GTTTTTCACG CAGTTTTTCA GGATGCAGCA AATCGGCGGC GCGAATGGCG 851 CGGCGTGCCA CTTTGTCTTC GTAGGCAGGG CCGATGCCGC GGCCGGTCGT 901 GCCGATTTTG CCTTTGCCGC GCGATGCTTC TCGGGCTTGG TCGAGCGCGA 951 TGTGATAAGG CAGGATCAGC GGGCAGGTCG GCGCGATTTT CAGACGGCCT 1001 TCGACGTTTT TCACGCCTGC CGCGTTCAAC TCGTCGATTT CGCCCAACAG 1051 GGCTTCGGGG GAGACGACAA CGCCCGAACC GATGAAGCAG TCCAGACTTT 1101 CATGCAGGAT GCCGCTCGGA ATCAGGCGCA AAATGGTTTT TTTGCCGCCG 1151 ACAACCAAGG TATGACCCGC ATTGTGGCCG CCTTGGAAGC GCACCACGCC 1201 TCCGGCTTCT TCCGCCAGCC AGTCAACGAT TTTACCTTTA CCCTCGTCGC 1251 CCCACTGTGC GCCGATTACT ACAACATTTT TAGCCATAGC CATATAACCT 1301 .TCGATATTA A This corresponds to the amino acid sequence <SEQ ID 2726; ORF 904.a>: a904.pep 1 MMQHNRFFAV GAGGDDGDRR TADFFNPFQI CFGIGR*CW AFHAESGFAP 51 TGHGFVNRLA GFYRIRAARQ DVGFAAVGQF VADADIDGFN AVHYIEFGNT 101 HTGNAVDLDG AFQGGGIKPA AAACASGYRT EFVSAFCQTC SDFVEQFGRE 151 RARTDARGIG FDDAQNIIQH LRAYARACRS RAGEAVGRSN EGVSAVVDVQ 201 QRTLRAFKQQ FFAVFVFFVQ HAGHVGNHRR NARRDFFDNR HHVFRFHRLG 251 IVQMLQLDW ISKDGIQFFT QFFRMQQIGG ANGAACHFVF VGRADAAAGR 301 ADFAFAARCF SGLVERDVIR QDQRAGRRDF QTAFDVFHAC RVQLVDFAQQ 351 GFGGDDNART DEAVQTFMQD AARNQAQNGF FAADNQGMTR IVAALEAHHA 401 SGFFRQPVND FTFTLVAPLC ADYYNIFSHS HITXRY* m904/a904 91.3% identity in 436 aa overlap 10 20 30 40 50 60 m904.pep MMQHNRFFSVGAGGDDGDRRAADFFNPFQICFGVFGQCAWLHAESGFAPAGHGFVNRLA I I II II II : II II I I I I II I : II I II II II II M I : I : : I I II I I I I : I I II II I I I a904 MMQHNRFFAVGAGGDDGDRRTADFFNPFQICFGIGRXCVVAFHAESGFAPTGHGFVNRLA 10 20 30 40 50 60 70 80 90 100 110 120 m904.pep GFHRIGTARQDVGFAAVGQFIADADIDGFNAVHYIEFSNTHTGNAVDLDGAFQGGGIKPA IMM : I II II II II I II M II I I II II II II II IM II I I I II II II I I I II II II II a904 GFYRIRAARQDVGFAAVGQFVADADIDGFNAVHYIEFGNTHTGNAVDLDGAFQGGGIKPA 70 80 90 100 110 120 130 140 150 160 170 180 m904.pep AAACASGYRTEFVSAFCQTYAYFVEQFGRERARTDARGIGFDDAQNIIQHLRTYARACRS I II II II II II II I II I II : II I II I I II II II I II II II I II I II II I I : I I I II II a904 AAACASGYRTEFVSAFCQTCSDFVEQFGRERARTDARGIGFDDAQNIIQHLRAYARACRΞ 130 140 150 160 170 180 190 200 210 220 230 240 m904. pep CARQTVGRGNEGISAWDVQQRTLRAFKQQFFAVFVFLVQHAGHVGNHRRNARRDFFDNR I : : I II : I II : I I I I II I II II li I I I II II I II I : I II II I II II I II I II II II II a904 RAGEAVGRSNEGVSAWDVQQRTLRAFKQQFFAVFVFFVQHAGHVGNHRRNARRDFFDNR 190 200 210 220 230 240 250 260 270 280 290 300 m904.pep HHVFRFNRLGIVQMLQLDIVIGKDGIQFFTQFXRMQQIGGANGAACHFVFVGRADAAAGR II I II I : II II II II II I : I M II I II I I II I II II II II I I I II I II II I I II II II I a904 HHVFRFHRLGIVQMLQLDWISKDGIQFFTQFFRMQQIGGANGAACHFVFVGRADAAAGR 250 260 270 280 290 300 310 320 330 340 350 360 m90 . pep ADFAFAAXIFAGLVERDWRQDQRAGRRDFQTAFDVFHACRVQLVDFAQQGFGGDDNART II I I II I I : II II II I : I II II II I I II I II II II II I II II II II II I II I II II II a904 ADFAFAARCFSGLVERDVIRQDQRAGRRDFQTAFDVFHACRVQLVDFAQQGFGGDDNART 310 320 330 340 350 360 370 380 390 400 410 420 m904. pep DEAVQTFMQDAARNQAQNGFFAADNQGMARIVAALEAHHAAGFFRQPVNDFTFTLVAPLC II I II II II II II II II I II I I II I II I : II II II I I II I : I II II II II II II II II II a904 DEAVQTFMQDAARNQAQNGFFAADNQGMTRIVAALEAHHASGFFRQPVNDFTFTLVAPLC 370 380 390 400 410 420 430 m904.pep ADXYNIFSHSHITYRYX II II I II I I II I III a904 ADYYNIFSHSHITXRYX 430 g906.seq not found yet g906.pep not found yet The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2727>: m906. seq 1 ATGAAATATA TCGTATCAAT CTCTCTGGCT ATGGGATTGG CTGCCTGTTC 51 GTTTGGGGGA TTTAAACCAA ATCCGTGGGA CGCCGCGTCA TTTTGGGAAT 101 TGAAAAATTA CGCCAATCCC TATCCGGGAT CAGCCTCGGC GGCACTTGAC 151 CAATATCCAT CGAAAGCAAG ACGAAGGCAA CTGAAAGACA TGCAAGAGTG 201 CGGCTATGAC CCAATAGACG GCGGAAAGTC TGAAGCAGAT GCCTGCCTGA 251 GGAAAAAAGG CTGGTGTCGT AAGGGTTTCG ACCCTTATCC CGAAAACAAA 301 AAATACGAAT GGCCTCGAGA AGAAGGAAAA ACAAAATGA This corresponds to the amino acid sequence <SEQ ID 2728; ORF 906>: m906.pep 1 MKYIVSISLA MGLAACSFGG FKPNPWDAAS FWELKNYANP YPGSASAALD 51 QYPSKARRRQ LKDMQECGYD PIDGGKSEAD ACLRKKGWCR KGFDPYPENK 101 KYEWPREEGK TK* The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2729>: g907.seq (partial) 1 ATGAAAAAAC CGACCGATAC CCTACCCGTC AATCTgcaAC GCCGCCGCCT 51 GCTGTGTGCC GCCGGCGCGC TGTTGATCAG CCCGCTGGCG CACGCCGGCG 101 CGCAACGTGA AGAAACGCtt gCCGACGATG TGGCTTCCGT GATGAGGAGT 151 TCTGTCGGCA GCGTCAATCC GCCGAGGCTG GTGTTCGACA ATCCGAAAGA 201 GGGCGAACGT TGGTTGTCCG CGATGTCGGC ACGTTTGGCA AGATTCGTCC 251 CCGACGAGGG GGAGCGGCGC AGGCTGCTGG TCAATATCCA ATACGAAAGC 301 AGCCGGGCCG GTTTGGATAC GCAGATTGTG TTGGGGCTGa ttgaagtgga 351 aagcgggtac cgagctcgaa tcatatca.. This corresponds to the amino acid sequence <SEQ ID 2730; ORF 907.ng>: g907.pep (partial) 1 MKKPTDTLPV ΝLQRRRLLCA AGALLISPLA HAGAQREETL ADDVASVMRS 51 SVGSVΝPPRL VFDΝPKEGER WLSAMSARLA RFVPDEGERR RLLVΝIQYES 101 SRAGLDTQIV LGLIEVESGY RARIIS... The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2731>: m907. seq 1 ATGAGAAAAC CGACCGATAC CCTACCCGTT AATCTGCAAC GCCGCCGCCT 51 GTTGTGTGCC GCCGGTGCGT TGTTGCTCAG TCCTCTGGCG CACGCCGGCG 101 CGCAACGTGA GGAAACGCTT GCCGACGATG TGGCTTCCGT GATGAGGAGT 151 TCTGTCGGCA GCGTCAATCC GCCGAGGCTG GTGTTTGACA ATCCGAAAGA 201 GGGCGAGCGT TGGTTGTCTG CCATGTCGGC ACGTTTGGCA AGGTTCGTCC 251 CCGAGGAGGA GGAGCGGCGC AGGCTGCTGG TCAATATCCA GTACGAAAGC 301 AGCCGGGCCG GTTTGGATAC GCAGATTGTG TTGGGGCTGA TTGAGGTGGA 351 AAGCGCGTTC CGCCAGTATG CAATCAGCGG TGTCGGCGCG CGCGGCCTGA 401 TGCAGGTTAT GCCGTTkTGG AAAAACTACA TCGGCAAACC GGCGCACAAC 451 CTGTTCGACA TCCGCACCAA CCTGCGTTAC GGCTGTACCA TCCTGCGCCA 501 TTACCGGAAT CTTGAAAAAG GCAACATCGT CCGCGCGCTT GCCCGCTTTA 551 ACGGCAGCTT GGGCAGCAAT AAATATCCGA ACGCCGTTTT GGgCGCGTGG 601 CGCAACCGCT GGCAGTGGCG TTGA This corresponds to the amino acid sequence <SEQ ID 2732; ORF 907>: m907.peρ 1 MRKPTDTLPV ΝLQRRRLLCA AGALLLSPLA HAGAQREETL ADDVASVMRS 51 SVGSVΝPPRL VFDΝPKEGER WLSAMSARLA RFVPEEEERR RLLVΝIQYES 101 SRAGLDTQIV LGLIEVESAF RQYAISGVGA RGLMQVMPXW KΝYIGKPAHΝ 151 LFDIRTNLRY GCTILRHYRN LEKGNIVRAL ARFNGSLGSN KYPNAVLGAW 201 RNRWQWR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 907 shows 92.9% identity over a 126 aa overlap with a predicted ORF (ORF 907.ng) from N gonorrhoeae: g907/m907 10 20 30 40 50 60 g907.pep MKKPTDTLPVΝLQRRRLLCAAGALLISPLAHAGAQREETLADDVASVMRSSVGSVΝPPRL I = 1111111111111111 f i 11111 ^ 11 i 1111 i 111111111111111111 j 11 M 11 j m907 MRKPTDTLPVΝLQRRRLLCAAGALLLSPLAHAGAQREETLADDVASVMRSSVGSVΝPPRL 10 20 30 40 50 60 70 80 90 100 110 120 g907.pep VFDΝPKEGERWLSAMSARLARFVPDEGERRRLLVΝIQYESSRAGLDTQIVLGLIEVESGY I I I I I I M I I 1 1 1 1 1 1 I I 1 1 I i 1 1 = I M I M M M M M M M M M M I I M M M = = m907 VFDΝPKEGERWLSAMSARLARFVPEEEERRRLLVΝIQYESSRAGLDTQIVLGLIEVESAF 70 80 90 100 110 120 g907.pep RARIIS I II m907 RQYAISGVGARGLMQVMPXWKΝYIGKPAHΝLFDIRTΝLRYGCTILRHYRΝLEKGΝIVRAL 130 140 150 160 170 180 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2733>: a907.seq 1 ATGAAAAAAC CGACCGATAC CCTACCCGTC AATCTGCAAC GCCGCCGCCT 51 ATTGTGTGCT GCCGGCGCGC TGTTGCTCAG CCCGCTGGCA CAAGCCGGCG 101 CGCAACGTGA AGAAACGCTT GCCGACGATG TGGCTTCCGT GATGAGGAGC 151 TCTGTCGGCA GCATAAATCC GCCGAGGCTG GTGTTCGACA ATCCGAAAGA 201 GGGCGAGCGT TGGCTGTCCG CGATGTCTGC TCGGTTGGCA AGGTTCGTCC 251 CCGATGAGGA GGAGCGGCGC AGGCTGCTGG TCAATATCCA GTACGAAAGC 301 AGCCGGGCCG GTTTGGATAC GCAGATTGTG TTGGGGCTGA TTGAGGTGGA 351 AAGCGCGTTC CGCCAGTATG CAATCAGCGG TGTCGGCGCG CGCGGCCTGA 401 TGCAGGTTAT GCCGTTTTGG AAAAACTACA TCGGCAAACC GGCGCACAAC 451 CTGTTCGACA TCCGCACCAA CCTGCGTTAC GGCTGTACCA TCCTGCGCCA 501 TTACCGGAAT CTTGAAAAAG GCAACATCGT CCGCGCACTC GCCCGTTTTA 551 ACGGTAGCCT CGGCAGCAAT AAATATCCGA ACGCCGTTTT GGGCGCGTGG 601 CGCAACCGCT GGCAGTGGCG TTGA This corresponds to the amino acid sequence <SEQ ID 2734; ORF 907. a>: a907.peρ 1 MKKPTDTLPV ΝLQRRRLLCA AGALLLSPLA QAGAQREETL ADDVASVMRS 51 SVGSIΝPPRL VFDΝPKEGER WLSAMSARLA RFVPDEEERR RLLVΝIQYES 101 SRAGLDTQIV LGLIEVESAF RQYAISGVGA RGLMQVMPFW KΝYIGKPAHΝ 151 LFDIRTNLRY GCTILRHYRN LEKGNIVRAL ARFNGSLGSN KYPNAVLGAW 201 RNRWQWR* m907/a907 97.6% identity in 207 aa overlap 10 20 30 40 50 60 m907.pep MRKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREETLADDVASVMRSSVGSVNPPRL M II I II II I I II II II II II I I II II I II : II II II I II II I II I II I II II M II I II a907 MKKPTDTLPVNLQRRRLLCAAGALLLSPLAQAGAQREETLADDVASVMRSSVGSINPPRL 10 20 30 40 50 60 70 80 90 100 110 120 m907.pep VFDNPKEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSRAGLDTQIVLGLIEVESAF MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a907 VFDNPKEGERWLSAMSARLARFVPDEEERRRLLVNIQYESSRAGLDTQIVLGLIEVESAF 70 10 90 100 110 120 130 140 150 160 170 180 m907 . pep RQYAISGVGARGLMQVMPXWKNYIGKPAHNLFDIRTNLRYGCTILRHYRNLEKGNIVRAL M M M M M M M M M I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M I I I I I I I I I a907 RQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRYGCTILRHYRNLEKGNIVRAL 130 140 150 160 170 180 190 200 m907. pep ARFNGSLGSNKYPNAVLGAWRNRWQWRX I II I II II II II II I II II II II I II I I a907 ARFNGSLGSNKYPNAVLGAWRNRWQWRX 190 200 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2735>: g908.seq 1 ATGAG.AAAA GCCGTCTAAG CCGGTATAAA CAAAATAAAC TCATTGGGCT 51 ATTTGTCGCA GGTGTAACTG CAAGAACAGC GGCAGAGTTG GTAGGCATTA 101 ATAAAAATAC CGCAGCCTAT GATTTTCATC GTTTACGATG ACTGATTTAT 151 CAAAACGGTC CGCATTTAGA AATGTTTGAT GGCGAAGTAG AAGCAGATGA 201 AAGTTATTTT GGCGGACAAC GCAAAGGCAA ACGCGGTCGC GGTGCTGCCG 251 GTAAAGTCGC CGTATTCGGT CTTTTGAAGC GAAATGGTAA GGTTTATACG 301 GTTACAGTAC CGAATACTCA AACCGCTACT TTATTTCCTA TTATCCGTGA 351 acaagtgaaa cctgacagta ttgtttatac ggattgttat CgTAGCTATG 401 ATGTATTAGA Tgtgagcgaa tttagccatT TTagcttcgc tgaaacttcg 451 ttttcgtaTC AATCACAGCA CACATTTTGC CGAACGACAA AACCATATTA 501 A This corresponds to the amino acid sequence <SEQ ID 2736; ORF 908.ng>: g908.pep 1 MXKSRLSRYK QΝKLIGLFVA GVTARTAAEL VGIΝKΝTAAY DFHRLR*LIY 51 QΝGPHLEMFD GEVEADESYF GGQRKGKRGR GAAGKVAVFG LLKRΝGKVYT 101 VTVPΝTQTAT LFPIIREQVK PDSIVYTDCY RSYDVLDVSE FSHFSFAETS 151 FSYQSQHTFC RTTKPY* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2737>: m908.seq 1 ATGAGAAAAA GTCGTCTAAG CCAGTATAAA CAAAmTAAAC TCATTGAACT 51 GTTTGTCACA GGTGTAACTG CAAGAACGGC AGCAGAGTTA GTAGGCGTTA 101 ATAAAAATAC CGCAGCCTAT TATTTTCATC GTTTACGATT ACTTATTTAT 151 CAAAACAGTC CGCATTTGGA AATGTTTGAT GGCGAAGTAG AAGCAGATGA 201 AAGTTATTTT GGCGGACAAC GCAAAGGCAA ACGCGGTCGC GGTGCTGCCG 251 GTAAAGTCGC CGTATTCGGT CTTTTGAAGC GAAATGGTAA GGTTTATACG 301 GTTACAGTAC CGAATACTCA AACCGCTACT TTATTTCCTA TTATCCGTGA 351 ACAAGTGAAA CCTGACAGCA TTTTTTATAC GGATTGTTAT CGTAGCTATG 401 ATGTATTAGA TGTGCGCGAA TTTAGCCATT TTAGCTTCGC TGAAACTTCG 451 TTTTCGTATC AATCACAGCA CACATTTTGC CGAACGACAA AACCATATTA 501 A This corresponds to the amino acid sequence <SEQ ID 2738; ORF 908>: m908.pep 1 MRKSRLSQYK QXKLIELFVT GVTARTAAEL VGVΝKΝTAAY YFHRLRLLIY 51 QΝSPHLEMFD GEVEADESYF GGQRKGKRGR GAAGKVAVFG LLKRΝGKVYT 101 VTVPΝTQTAT LFPIIREQVK PDSIFYTDCY RSYDVLDVRE FSHFSFAETS 151 FSYQSQHTFC RTTKPY* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 908 shows 93.4% identity over a 166 aa overlap with a predicted ORF (ORF 908. ng) from N gonorrhoeae: g908/m908 10 20 30 40 50 60 g908.pep MXKSRLSRYKQΝKLIGLFVAGVTARTAAELVGIΝKΝTAAYDFHRLRXLIYQΝGPHLEMFD m908 MRKSRLSQYKQXKLIELFVTGVTARTAAELVGVNKNTAAYYFHRLRLLIYQNSPHLEMFD 10 20 30 40 50 60 70 80 90 100 110 120 g908.pep GEVEADESYFGGQRKGKRGRGAAGKVAVFGLLKRNGKVYTVTVPNTQTATLFPIIREQVK MMMIMMMMMMMMMMMMMMMMMMMIMMMMMMM m908 GEVEADESYFGGQRKGKRGRGAAGKVAVFGLLKRNGKVYTVTVPNTQTATLFPIIREQVK 70 80 90 100 110 120 130 140 150 160 g908.pep PDSIVYTDCYRSYDVLDVSEFSHFSFAETSFSYQSQHTFCRTTKPYX l l l l M M M I M M M I I I I I I I I I 1 1 I I I I I I I I I I I 1 1 1 1 1 1 m908 PDSIFYTDCYRSYDVLDVREFSHFSFAETSFSYQSQHTFCRTTKPYX 130 140 150 160 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2739>: a908.seq 1 ATGAGAAAAA GTCGTCTAAG CCAGTATAAA CAAAATAAAC TCATTGAGCT 51 ATTTGTCGCA GGTGTAACTG CAAGAACGGC AGCAGAGTTA GTAGGCGTTA 101 ATAAAAATAC CGCAGCCTAT TATTTTCATC GTTTACGATT ACTTATTTAT 151 CAAAACAGTC CGCATTTGGA AATGTTTGAT GGCGAAGTAG AAGCAGATGA 201 AAGTTATTTT GGCGGACAAC GCAAAGGCAA ACGCGGTCGC GGTGCTGCCG 251 GTAAAGTCGC CGTATTCGGT CTTTTGAAGC GAAATGGTAA GGTTTATACG 301 GTTACAGTAC CGAATACTCA AACCGCTACT TTATTTCCTA TTATCCGTGA 351 ACAAGTGAAA CCTGACAGCA TTGTTTATAC GGATTGTTAT CGTAGCTATG 401 ATGTATTAGA TGTGCGCGAA TTTAGCCATT TTAGCTTCGC TGAAACTTCG 451 TTTTCGTATC AATCACAGCA CACATTTTGC CGAACGACAA AACCATATTA 501 A This corresponds to the amino acid sequence <SEQ ID 2740; ORF 908.a>: a908.pep 1 MRKSRLSQYK QΝKLIELFVA GVTARTAAEL VGVΝKΝTAAY YFHRLRLLIY 51 QΝSPHLEMFD GEVEADESYF GGQRKGKRGR GAAGKVAVFG LLKRΝGKVYT 101 VTVPΝTQTAT LFPIIREQVK PDSIVYTDCY RSYDVLDVRE FSHFSFAETS 151 FSYQSQHTFC RTTKPY* m908/a908 98.2% identity in 166 aa overlap 10 20 30 40 50 60 m908.pep MRKSRLSQYKQXKLIELFVTGVTARTAAELVGVΝKΝTAAYYFHRLRLLIYQΝSPHLEMFD I I I II II I I II II II I I I : I I II I II I II II II II II II II I II I I II I II I II I II II a908 MRKSRLSQYKQΝKLIELFVAGVTARTAAELVGVΝKΝTAAYYFHRLRLLIYQΝSPHLEMFD 10 20 30 40 50 60 70 80 90 100 110 120 m908. pep GEVEADESYFGGQRKGKRGRGAAGKVAVFGLLKRΝGKVYTVTVPΝTQTATLFPIIREQVK I II II II II II I II II I II I II I II I II I I II I I II I I I II I I II I II I II II II II I II a908 GEVEADESYFGGQRKGKRGRGAAGKVAVFGLLKRΝGKVYTVTVPΝTQTATLFPIIREQVK 70 80 90 100 110 120 130 140 150 160 m908.pep PDSIFYTDCYRSYDVLDVREFSHFSFAETSFSYQSQHTFCRTTKPYX II II I II I I II I I I II II I I II II I I I II I I I II I II II I II I II I a908 PDSIVYTDCYRSYDVLDVREFSHFSFAETSFSYQSQHTFCRTTKPYX 130 140 150 160 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 2741>: g909.seq (partial) 1 atgcgtaaaa ccgtacttat cCTgaccatc tccgccgccc ttttgtcggg 51 ctgcacatgG gaaacttatc aagacggcag cggcaaaacc gccgtccgtg 101 caaaatgttc caccggcacg ccgctgtgtt ggcaagacgg gcgcggctcg 151 aaaaaggtgg actgcgacga gtacggtggc gaacgccggg ccgtgttgcg 201 caaccaaaag cgggggaagc ccgcgacgag gagagccgca acgctgggga 251 aaccgagttt ccgggcgagg gacggggggg ggcgggtgaa cagggcagaa 301 acgggggagg ggaagcgatc ggcgagg.. This corresponds to the amino acid sequence <SEQ ID 2742; ORF 909.ng>: g909.pep (partial) 1 MRKTVLILTI SAALLSGCTW ETYQDGSGKT AVRAKCSTGT PLCWQDGRGS 51 KKVDCDEYGG ERRAVLRNQK RGKPATRRAA TLGKPSFRAR DGGGRVNRAE 101 TGEGKRSAR.. The following partial DNA sequence was identified in N meningitidis <SEQ ID 2743>: m909.seq 1 ATGCGTAAAA CCTTCCTCTT CCTGACCGCT GCCGCCGCCC TTTTGTCGGG 51 CTGCGCGTGG GAAACTTATC AAGACGGCAA CGGCAAGACC GCCGTCCGTC 101 AAAAATATCC CGCCGGCACG CCCGTTTATT ACCAAGACGG CAGCTACTCG 151 AAAAATATGA ACTACAACCA ATACCGTCCC GAACGCCATG CCGTGTTACC 201 CAATCAAACC GGCAACAACG CCGACGAAGA GCATCGCCAA CACTGGCAAA 251 AACCAAAGTT TCAAAACCGA TAA This corresponds to the amino acid sequence <SEQ ID 2744; ORF 909>: m909.pep 1 MRKTFLFLTA AAALLSGCAW ETYQDGΝGKT AVRQKYPAGT PVYYQDGSYS 51 KΝMΝYΝQYRP ERHAVLPΝQT GΝΝADEEHRQ HWQKPKFQΝR * Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 909 shows 53.3% identity over a 90 aa overlap with a predicted ORF (ORF 909.ng) from N gonorrhoeae: m909/g909 10 20 30 40 50 60 m909.pep MRKTFLFLTAAAALLSGCAWETYQDGΝGKTAVRQKYPAGTPVYYQDGSYSKΝMΝYΝQYRP M M h l l M l l l l l h l l l l l l h l l M I I I = 1 1 1 = = 1 1 1 l h = = = = l g909 MRKTVLILTISAALLSGCTWETYQDGSGKTAVRAKCSTGTPLCWQDGRGSKKVDCDEYGG 10 20 30 40 50 60 70 80 90 m909.pep ERHAVLPΝQTGΝΝADEEHRQHWQKPKFQΝRX II =111 II == == 11=1= I g909 ERRAVLRΝQKRGKPATRRAATLGKPSFRARDGGGRVΝRAETGEGKRSAR 70 80 90 100 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2745>: a909.seq 1 ATGCGTAAAA CCTTCCTTAT CCTGATGACT GCCGCCGCCC TTTTGTCGGG 51 CTGCGCGTGG GAAACTTATC AAGACGGCAA CGGCAAGACC GCCGTCCGTC 101 AAAAATATCC CGCCGGCACG CCCGTTTATT ACCAAGACGG CAGCTACTCG 151 AAAAATATGA ACTACAACCA ATACCGTCCC GAACGCCATG CCGTGTTACC 201 CAACCAAACC GGCAACAACG CCGACGAAGA GCATCGCCAA CACTGGCAAA 251 AGCCCAAATT TCAAAACCGA TAA This corresponds to the amino acid sequence <SEQ ID 2746; ORF 909.a>: a909.pep 1 MRKTFLILMT AAALLSGCAW ETYQDGΝGKT AVRQKYPAGT PVYYQDGSYS 51 KΝMΝYΝQYRP ERHAVLPΝQT GΝΝADEEHRQ HWQKPKFQΝR * m909/a909 96.7% identity in 90 aa overlap 10 20 30 40 50 60 m909. pep MRKTFLFLTAAAALLSGCAWETYQDGΝGKTAVRQKYPAGTPVYYQDGSYSKΝMΝYΝQYRP I II II IM : II II II II II II II I II I I I I II I I I I I I II I I I I I I I I I II I I I II II I a909 MRKTFLILMTAAALLSGCAWETYQDGΝGKTAVRQKYPAGTPVYYQDGSYSKΝMΝYΝQYRP 10 20 30 40 50 60 70 80 90 m909. pep ERHAVLPNQTGNNADEEHRQHWQKPKFQNRX I II I I I II I II II I I II I I I I II II II I I I I a909 ERHAVLPNQTGNNADEEHRQHWQKPKFQNRX 70 80 90 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 2747>: g910.seq 1 ATGAAAAAAC TGTTATTGGC CGCCGTTGTT TCCCTAAATG CCGCAACCGC 51 ATTTGCCGGC GACTCTGCCG AGCGTCAGAT TTACGGCGAT CCCCATTTTG 101 AACAAAACCG CACAAAAGCC GTGAAAATGT TGGAACAGCG CGGTTATCAG 151 GTTTACGATG TCGATGCCGA CGACTACTGG GGCAAACCTG TTTTGGAAGT 201 GGAAGCCTAT AAAGACGGCC GCGAATACGA CATCGTGTTG TCTTACCCCG 251 ACCTGAAAAT CATCAAAGAG CAGCTCGATC GCTGA This corresponds to the amino acid sequence <SEQ ID 2748; ORF 910.ng>: g910.pep 1 MKKLLLAAW SLΝAATAFAG DSAERQIYGD PHFEQΝRTKA VKMLEQRGYQ 51 VYDVDADDYW GKPVLEVEAY KDGREYDIVL SYPDLKIIKE QLDR* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2749>: m910. seq 1 ATGAAAAAAC TGTTATTGGC TGCCGTTGTT TCTCTGAGTG CCGCTGCCGC 51 ATTTGCCGGC GACTCTGCCG AGCGTCAGAT TTACGGCGAT CCCCATTTTG 101 AACAAAACCG CACAAAAGCT GTGAAAATGT TGGAGCAGCG CGGTTATCAG 151 GTTTACGATG TCGATGCCGA CGACCATTGG GGTAAGCCTG TGCTGGAAGT 201 GGAAGCCTAT AAAGACGGCC GCGAATACGA CATCGTGTTG TCTTACCCCG 251 ACCTGAAAAT CATCAAAGAG CAGCTCGATC GCTGA This corresponds to the amino acid sequence <SEQ ID 2750; ORF 910>: m910.pep 1 MKKLLLAAW SLSAAAAFAG DSAERQIYGD PHFEQΝRTKA VKMLEQRGYQ 51 VYDVDADDHW GKPVLEVEAY KDGREYDIVL SYPDLKIIKE QLDR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 910 shows 96.8% identity over a 94 aa overlap with a predicted ORF (ORF 910.ng) from N gonorrhoeae: g910/m910 10 20 30 40 50 60 g910 . pep MKKLLLAAWSLΝAATAFAGDSAERQI YGDPHFEQΝRTKAVKMLEQRGYQVYDVDADDYW m910 MKKLLLAAWSLSAAAAFAGDSAERQIYGDPHFEQΝRTKAVKMLEQRGYQVYDVDADDHW 10 20 30 40 50 60 70 80 90 g910.pep GKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDRX MMMMMMIMMMMMIMMMMMI m910 GKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDRX 70 80 90 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2751>: a910.seq 1 ATGAAAAAAC TGTTATTGGT CGCCGTTGTT TCCTTGAGTG CCGCAACCGC 51 ATTTGCCGGC GACTCTGCCG AGCGTCAGAT TTACGGCGAT CCCTATTTTG 101 AACAAAACCG CACAAAAGCC GTGAAAATGT TGGAACAGCG CGGTTATCAG 151 GTTCACGATG TCGATGCCGA CGACCATTGG GGCAAACCTG TTTTGGAAGT 201 GGAAGCCTAT AAAGACGGCC GCGAATACGA CATTGTGTTG TCTTACCCCG 251 ACCTGAAAAT CATCAAAGAG CAGCTCGATC GCTGA This corresponds to the amino acid sequence <SEQ ID 2752; ORF 910.a>: a910 . pep 1 MKKLLLVAVV SLSAATAFAG DSAERQIYGD PYFEQNRTKA VKMLEQRGYQ 51 VHDVDADDHW GKPVLEVEAY KDGREYDIVL SYPDLKIIKE QLDR* m910/a910 95.7% identity in 94 aa overlap 10 20 30 40 50 60 m910 . pep MKKLLLAAWSLSAAAAFAGDSAERQIYGDPHFEQNRTKAVKMLEQRGYQVYDVDADDHW I I I I I I I I I I I I M I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I : I I I I I I I I a910 MKKLLLVAVVSLSAATAFAGDSAERQIYGDPYFEQNRTKAVKMLEQRGYQVHDVDADDHW 10 20 30 40 50 60 70 80 90 m910.pep GKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDRX II I II II II II II I II II I II II II I II I I II II I a910 GKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDRX 70 80 90 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2753>: g911.seq 1 ATGAAAAAGA ACATATTGGA ATTTTGGGTC GGACTGTTCG TCTTGATCGG 51 CGCGGCGGCG GTTGCCTTTC TCGCTTTCCG CGTGGCGGGC GGCGCGGCGT 101 TCGGCGGTTC GGACAAAACT TACGCCGTTT ATGCCGATTT CGGCGACATC 151 GGCGGTTTGA AGGTCAATGC CCCCGTCAAA TCCGCAGGCG TATTGGTCGG 201 GCGCGTCGGC GCTATCGGGC TTGACCCGAA ATCCTATCAG GCGAGGGTGC 251 GCCTTGATTT GGACGGCAAG TATCAGTTCA GCAGTGACGT TTCCGCGCAA 301 ATCCTGACTT CGGGACTTTT GGGCGAACAG TACATCGGGC TGCAGCAGGG 351 CGGCGATACG GAAAACCTTG CTGCCGGCGA CACCATCTCC GTAACCAGTT 401 CTGCAATGGT TCTGGAAAAC CTGATCGGTA AATTCATGAC CAGCTTCGCC 451 GAGAAAAACG CTGAGGGCGG CAATGCGGAA AAAGCCGcag aAtaa This corresponds to the amino acid sequence <SEQ ID 2754; ORF 911.ng>: g911.pep 1 MKKΝILEFWV GLFVLIGAAA VAFLAFRVAG GAAFGGSDKT YAVYADFGDI 51 GGLKVΝAPVK SAGVLVGRVG AIGLDPKSYQ ARVRLDLDGK YQFSSDVSAQ 101 ILTSGLLGEQ YIGLQQGGDT EΝLAAGDTIS VTSSAMVLEΝ LIGKFMTSFA 151 EKΝAEGGΝAE KAAE* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2755>: m911.seq 1 ATGAAGAAGA ACATATTGGA ATTTTGGGTC GGACTGTTCG TCCTGATTGG 51 CGCGGCGGCG GTTGCCTTTC TCGCTTTCCG CGTGGCCGGC GGTGCGGCGT 101 TCGGCGGTTC GGACAAAACT TACGCCGTTT ATGCCGATTT CGGCGACATC 151 GGCGGTTTGA AGGTCAATGC CCCCGTCAAA TCCGCAGGCG TATTGGTCGG 201 GCGCGTCGGC GCTATCGGAC TTGACCCGAA ATCCTATCAG GCGAGGGTGC 251 GCCTCGATTT GGACGGCAAG TATCAGTTCA GCAGCGACGT TTCCGCGCAA 301 ATCCTGACTT CGGGACTTTT GGGCGAGCAG TACATCGGGC TGCAGCAGGG 351 CGGCGACACG GAAAACCTTG CTGCCGGCGA CACCATCTCC GTAACCAGTT 401 CTGCAATGGT TCTGGAAAAC CTTATCGGCA AATTCATGAC GAGTTTTGCC 451 GAGAAAAATG CCGACGGCGG CAATGCGGAA AAAGCCGCCG AATAA This corresponds to the amino acid sequence <SEQ ID 2756; ORF 911>: m911.pep 1 MKKΝILEFWV GLFVLIGAAA VAFLAFRVAG GAAFGGSDKT YAVYADFGDI 51 GGLKVΝAPVK SAGVLVGRVG AIGLDPKSYQ ARVRLDLDGK YQFSSDVSAQ 101 ILTSGLLGEQ YIGLQQGGDT EΝLAAGDTIS VTSSAMVLEΝ LIGKFMTSFA 151 EKΝADGGΝAE KAAE* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 911 shows 99.4% identity over a 164 aa overlap with a predicted ORF (ORF 91 l.ng) from N gonorrhoeae: g911/m911 10 20 30 40 50 60 g911.pep MKKNILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGGSDKTYAVYADFGDIGGLKVNAPVK MMMMMMMMMMIMMMMMMMMMMMMMMIIMIMMM m911 MKKNILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGGSDKTYAVYADFGDIGGLKVNAPVK 10 20 30 40 50 60 70 80 90 100 110 120 g911. pep SAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQILTSGLLGEQYIGLQQGGDT MMMMMMMMIMMMMMMMMMMMMMMMMMIMMMM m911 SAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQILTSGLLGEQYIGLQQGGDT 70 80 90 100 110 120 130 140 150 160 g911.pep ENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNAEGGNAEKAAEX m911 ENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAEKAAEX 130 140 150 160 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2757>: a911. seq 1 ATGAAAAAGA ACATATTGGA ATTTTGGGTC GGACTGTTCG TCCTGATTGG 51 CGCGGCGGCG GTTGCCTTTC TCGCTTTCCG CGTGGCCGGC GGTGCGGCGT 101 TCGGCGGTTC GGACAAAACT TACGCCGTTT ATGCCGATTT CGGCGACATC 151 GGCGGTTTGA AGGTCAATGC CCCCGTCAAA TCCGCAGGCG TATTGGTCGG 201 GCGCGTCGGC GCTATCGGAC TTGACCCGAA ATCCTATCAG GCGAGGGTGC 251 GCCTCGATTT GGACGGCAAG TATCAGTTCA GCAGCGACGT TTCCGCGCAA 301 ATCCTGACTT CGGGACTTTT GGGCGAGCAG TACATCGGGC TGCAGCAGGG 351 CGGCGACACG GAAAACCTTG CTGCCGGCGA CACCATCTCC GTAACCAGTT 401 CTGCAATGGT TCTGGAAAAC CTTATCGGCA AATTCATGAC GAGTTTTGCC 451 GAGAAAAATG CCGACGGCGG CAATGCGGAA AAAGCCGCCG AATAA This corresponds to the amino acid sequence <SEQ ID 2758; ORF 91 l.a>: a911.pep 1 MKKΝILEFWV GLFVLIGAAA VAFLAFRVAG GAAFGGSDKT YAVYADFGDI 51 GGLKVΝAPVK SAGVLVGRVG AIGLDPKSYQ ARVRLDLDGK YQFSSDVSAQ 101 ILTSGLLGEQ YIGLQQGGDT EΝLAAGDTIS VTSSAMVLEΝ LIGKFMTSFA 151 EKΝADGGΝAE KAAE* m911/a911 100.0% identity in 164 aa overlap 10 20 30 40 50 60 ιtι911 . pep MKKΝILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGGSDKTYAVYADFGDIGGLKVΝAPVK MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a911 MKKΝILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGGSDKTYAVYADFGDIGGLKVΝAPVK 10 20 30 40 50 60 70 80 90 100 110 120 m911. pep SAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQILTSGLLGEQYIGLQQGGDT II I II I II II II I I II II II I II II II I II II I II I II II I II II II I II I I II I II I I I a911 SAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQILTSGLLGEQYIGLQQGGDT 70 80 90 100 110 120 130 140 150 160 m911.pep EΝLAAGDTISVTSSAMVLEΝLIGKFMTSFAEKΝADGGΝAEKAAEX I II I II II II I II I II I II II I II I II I I I I I I II II II II II II a911 EΝLAAGDTISVTSSAMVLEΝLIGKFMTSFAEKΝADGGΝAEKAAEX 130 140 150 160 The following partial DΝA sequence was identified in N. gonorrhoeae <SEQ ID 2759>: g912.seq 1 gtgAAAAaat cctcctTcat cagcGCATTG GGCATCGgtA TTTTGAGCAT 51 CGGCATGGCA TTTGCCTCCC CGGCCGACGC AGTGGGACAA ATCCGCCAAA 101 ACGCCACACA GGTTTTGACC ATCCTCAAAA GCGGCGACGC GGCTTCTGCA 151 CGCCCAAAAG CCGAAGCCTA TGCGGTTCCC TATTTCGATT TCCAACGTAT 201 GACCGCATTG GCGGTCGGCA ACCCTTGGCG TACCGCGTCC GACGCGCAAA 251 AACAAGCGTT GGCCAAAGAA TTTCAAACCC TGCTGATCCG CACCTATTCC 301 GGCACGATGC TGAAATTCAA AAACGCGACC GTCAACGTCA AAGACAATCC 351 CATCGTCAAT AAGGGCGGCA AGGAAATCGT CGTCCGTGCC GAAGTCGGCA 401 TCCCCGGTCA GAAGCCCGTC AATATGGACT TTACCACCTA CCAAAGCGGC 451 GGCAAATACC GTACCTACAA CGTCGCCATC GAAGGCACGA GCCTGGTTAC 501 CGTGTACCGC AACCAATTCG GCGAAATCAT CAAAGCCAAA GGCATCGACG 551 GGCTGATTGC CGAGTTGAAA GCCAAAAACG GCGGCAAATA A This corresponds to the amino acid sequence <SEQ ID 2760; ORF 912.ng>: g912.pep 1 VKKSSFISAL GIGILSIGMA FASPADAVGQ IRQNATQVLT ILKSGDAASA 51 RPKAEAYAVP YFDFQRMTAL AVGNPWRTAS DAQKQALAKE FQTLLIRTYS 101 GTMLKFKNAT VNVKDNPIVN KGGKEIWRA EVGIPGQKPV NMDFTTYQSG 151 GKYRTYNVAI EGTSLVTVYR NQFGEIIKAK GIDGLIAELK AKNGGK* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2761>: m912.seq 1 ATGAAAAAAT CCTCCCTCAT CAGCGCATTG GGCATCGGTA TTTTGAGCAT 51 CGGCATGGCA TTTGCCGCCC CTGCCGACGC GGTAAGCCAA ATCCGTCAAA 101 ACGCCACTCA AGTATTGAGC ATCTTAAAAA ACGGCGATGC CAACACCGCT 151 CGCCAAAAAG CCGAAGCCTA TGCGATTCCC TATTTCGATT TCCAACGTAT 201 GACCGCATTG GCGGTCGGCA ACCCTTGGCG CACCGCGTCC GACGCGCAAA 251 AACAAGCGTT GGCCAAAGAA TTTCAAACCC TGCTGATCCG CACCTATTCC 301 GGCACGATGC TGAAATTAAA AAACGCCAAC GTCAACGTCA AAGACAATCC 351 CATCGTCAAT AAAGGCGGCA AAGAAATCAT CGTCCGCGCC GAAGTCGGCG 401 TACCCGGGCA AAAACCCGTC AACATGGACT TCACCACCTA CCAAAGCGGC 451 GGTAAATACC GTACCTACAA CGTCGCCATC GAAGGCGCGA GCCTGGTTAC 501 CGTGTACCGC AACCAATTCG GCGAAATTAT CAAAGCGAAA GGCGTGGACG 551 GACTGATTGC CGAGTTGAAA GCCAAAAACG GCGGCAAATA A This corresponds to the amino acid sequence <SEQ ID 2762; ORF 912>: m912.pep 1 MKKSSLISAL GIGILSIGMA FAAPADAVSQ IRQΝATQVLS ILKΝGDAΝTA 51 RQKAEAYAIP YFDFQRMTAL AVGNPWRTAS DAQKQALAKE FQTLLIRTYS 101 GTMLKLKNAN VNVKDNPIVN KGGKEIIVRA EVGVPGQKPV NMDFTTYQSG 151 GKYRTYNVAI EGASLVTVYR NQFGEIIKAK GVDGLIAELK AKNGGK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 912 shows 91.8% identity over a 196 aa overlap with a predicted ORF (ORF 912.ng) from N gonorrhoeae: g912/m912 10 20 30 40 50 60 g912.pep VKKSSFISALGIGILSIGMAFASPADAVGQIRQΝATQVLTILKSGDAASARPKAEAYAVP = 1 1 l l l l l h l m912 MKKSSLISALGIGILSIGMAFAAPADAVSQIRQΝATQVLSILKΝGDAΝTARQKAEAYAIP 10 20 30 40 50 60 70 80 90 100 110 120 g912.pep YFDFQRMTALAVGΝPWRTASDAQKQALAKEFQTLLIRTYSGTMLKFKΝATVΝVKDΝPIVΝ m912 YFDFQRMTALAVGΝPWRTASDAQKQALAKEFQTLLIRTYSGTMLKLKΝAΝVΝVKDΝPIVΝ 70 80 90 100 110 120 130 140 150 160 170 180 g912 . pep KGGKEIWRAEVGIPGQKPVΝMDFTTYQSGGKYRTYΝVAIEGTSLVTVYRΝQFGEI IKAK M M I h l M M h l M M M M M M M I M M M M M I h l M M M M M M M M m912 KGGKEIIVRAEVGVPGQKPVΝMDFTTYQSGGKYRTYΝVAIEGASLVTVYRΝQFGEIIKAK 130 140 150 160 170 180 190 g912.pep GIDGLIAELKAKΝGGKX m912 GVDGLIAELKAKNGGKX 190 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2763>: a912.seq 1 ATGAAAAAAT CCTCCTTCAT CAGCGCATTG GGCATCGGTA TTTTGAGCAT 51 CGGCATGGCA TTTGCCGCCC CTGCCGACGC GGTAAACCAA ATCCGTCAAA 101 ACGCCACTCA AGTATTGAGC ATCTTAAAAA GCGGTGATGC CAACACCGCC 151 CGCCAAAAAG CCGAAGCCTA TGCGATTCCC TATTTCGATT TCCAACGTAT 201 GACCGCATTG GCGGTCGGCA ACCCTTGGCG CACCGCGTCC GACGCGCAAA 251 AACAAGCGTT GGCCAAAGAA TTTCAAACCC TGCTGATCCG CACCTATTCC 301 GGCACGATGC TGAAATTAAA AAACGCCAAC GTCAACGTCA AAGACAATCC 351 CATCGTCAAT AAAGGCGGCA AAGAAATCAT CGTCCGCGCC GAAGTCGGCG 401 TACCCGGGCA AAAACCCGTC AACATGGACT TCACCACCTA CCAAAGCGGC 451 GGTAAATACC GTACCTACAA CGTCGCCATC GAAGGCGCGA GCCTGGTTAC 501 CGTGTACCGC AACCAATTCG GCGAAATTAT CAAAGCGAAA GGCGTGGACG 551 GACTGATTGC CGAGTTGAAG GCTAAAAACG GCAGCAAGTA A This corresponds to the amino acid sequence <SEQ ID 2764; ORF 912.a>: a912.pep 1 MKKSSFISAL GIGILSIGMA FAAPADAVΝQ IRQΝATQVLS ILKSGDAΝTA 51 RQKAEAYAIP YFDFQRMTAL AVGNPWRTAS DAQKQALAKE FQTLLIRTYS 101 GTMLKLKNAN VNVKDNPIVN KGGKEIIVRA EVGVPGQKPV NMDFTTYQSG 151 GKYRTYNVAI EGASLVTVYR NQFGEIIKAK GVDGLIAELK AKNGSK* m912/a912 98.0% identity in 196 aa overlap 10 20 30 40 50 60 m912 . pep MKKSSLISALGIGILSIGMAFAAPADAVSQIRQNATQVLSILKNGDANTARQKAEAYAIP M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M a912 MKKSSFISALGIGILSIGMAFAAPADAVNQIRQNATQVLSILKSGDANTARQKAEAYAIP 10 20 30 40 50 60 70 80 90 100 110 120 m912. pep YFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGTMLKLKNANVNVKDNPIVN I I I II I II II I I II II II II II II II II II II II I II I II I II I I I II I II II I II II II a912 YFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGTMLKLKNANVNVKDNPIVN 70 80 90 100 110 120 130 140 150 160 170 180 m912.pep KGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAIEGASLVTVYRNQFGEIIKAK II II II I II II I I II II II II II II I II II II II I I II I II II I II II I II I II II II II a912 KGGKE11VRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAIEGASLVTVYRNQFGEIIKAK 130 140 150 160 170 180 190 m912. pep GVDGLIAELKAKNGGKX I II II II II II II M II a912 GVDGLIAELKAKNGSKX 190 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2765>: g913.seq 1 atGAAAAAAA CCGCCTACGC CATCCTCCTG CTGATCGGGT TCGCTTCCGC 51 CCCTGCATTT GCAGAAACCC GCCCCGCCGA CCCTTATGAA GGCTACAACC 101 GCGCCGTTTC CAAATTCAAC GACCAAGCCG ACCGCTACAT TTTCGCCCCT 151 GCCGCGCGCG GCTACCGCAA AGTTACGCCG AAACCCGTCC GCGCCGGCGT 201 GTCCAATTTT TTTAACAACC TGCGCGACGT GGTCAGTTTC GGCAGCAATA 251 TCTTGCGTTT GGAcatCAAA cgcgcAAGcg aAGACCtcgT CCGcgtcggc 301 atCAATACCA CCTTCGGTTT GGgcgGGCTC ATTGATATTG CCGGcgcGGg 351 cggcgttccc gacaataaaa AcacTttgGg cgacacgttt gcctcgtGGG 401 GctgGAAAaa cagcaATTAT TTCGTgttgc CCGtcttagg cccgtccacc 451 gtccgcgacg cgctcggcac gggcattacc tCTGTTTATC CGCccaagaa 501 tatcgttttc catacccctg ccggacgctg GGgcacgact gCCGCTGCCG 551 CCGTcagtac gcgcgaaggc ctcctcgatt tgaccgacag TCtggacgaa 601 gccgccatCG ACAAATACAG CTACACGCGc gacctctata tgAAAGTCCG 651 CGcacgGCag AccgGTGCAA CACCTGCCGA AGgtacggaa gataacatcg 701 acatcgacat cgACGAATTG GTCGAAAGTG CCGAAACCGG CGCGGCAGAG 751 CCCGCCGTTC ACGAAGATTC CGTATCCGAA ACACAGGCAG AAGCAGCAGG 801 GGAAGCCGAA ACGCAACCTG GAACACAACC CTAA This corresponds to the amino acid sequence <SEQ ID 2766; ORF 913.ng>: g913.pep 1 MKKTAYAILL LIGFASAPAF AETRPADPYE GYNRAVSKFN DQADRYIFAP 51 AARGYRKVTP KPVRAGVSNF FN LRD SF GSNILRLDIK RASEDLVRVG 101 INTTFGLGGL IDIAGAGGVP DNKNTLGDTF ASWGWKNSNY FVLPVLGPST 151 VRDALGTGIT SVYPPKNIVF HTPAGRWGTT AAAAVSTREG LLDLTDSLDE 201 AAIDKYSYTR DLYMKVRARQ TGATPAEGTE DNIDIDIDEL VESAETGAAE 251 PAVHEDSVSE TQAEAAGEAE TQPGTQP* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2767>: m913.seq 1 ATGAAAAAAA CCGCCTATGC CTTCCTCCTG CTGATCGGGT TCGCTTCCGC 51 CCCTGCATTT GCCGAAACCC GCCCCGCCGA CCCTTATGAA GGCTACAACC 101 GCGCCGTTTT CAAATTCAAC GACCAAGCCG ACCGCTACAT TTTCGCCCCT 151 GCCGCGCGCG GCTACCGCAA AGTTGCGCCG AAACCCGTCC GCGCCGGCGT 201 GTCCAATTTT TTTAACAACC TGTGCGACGT GGTCAGCTTC GGCAGCAATA 251 TCTTGCGCTT GGACATCAAA CGCGCAAGCG AAGACCTTGT CCGCGTCGGC 301 ATCAACACCA CTTTCGGTTT GGGCGGGCTT ATCGACATCG CCGGCGCGGG 351 CGGCATTCCC GACAATAAAA ACACCTTGGG CGACACGTTT GCCTCGTGGG 401 GATGGAAAAA CAGCAATTAT TTCGTGTTGC CCGTCTTAGG GCCGTCCACC 451 GTCCGCGACG CGCTCGGCAC GGGTATTACC TCCGTTTATT CGCCCAAGAA 501 TATCGTCTTC CGCACCCCTG TCGGACGCTG GGGCACGACT GCCGTATCCG 551 CCGTCAGTAC GCGCGAAGGC CTgCTCGATT TGACCGACAG TCTGGACGAA 601 GCCGCCATCG ACAAATACAG CTACACGCGC GACCTCTATA TGAAAGTCCG 651 TGCGCGGCAG ACCGGTGCAA CACCTGCCGA AGgTACGGAA GATAACATCG 701 ACATCGACGA ATTGGTCGAA AGTGCCGAAA CCGGCGCGGC GGAAACTGCC 751 GTTCAAGAAG ATTCCGTATC CGAAACACAG GCAGAAGCAG CAGGGGAAGC 801 CGAAACGCAA CCTGGAACAC AACCTTAA This corresponds to the amino acid sequence <SEQ ID 2768; ORF 913>: m913.pep 1 MKKTAYAFLL LIGFASAPAF AETRPADPYE GYΝRAVFKFΝ DQADRYIFAP 51 AARGYRKVAP KPVRAGVSNF FNNLCDWSF GSNILRLDIK RASEDLVRVG 101 INTTFGLGGL IDIAGAGGIP DNKNTLGDTF ASWGWKNSNY FVLPVLGPST 151 VRDALGTGIT SVYSPKNIVF RTPVGRWGTT AVSAVSTREG LLDLTDSLDE 201 AAIDKYSYTR DLYMKVRARQ TGATPAEGTE DNIDIDELVE SAETGAAETA 251 VQEDSVSETQ AEAAGEAETQ PGTQP* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 913 shows 94.9% identity over a 277 aa overlap with a predicted ORF (ORF 913.ng) from N gonorrhoeae: g913/m913 10 20 30 40 50 60 g913.pep MKKTAYAILLLIGFASAPAFAETRPADPYEGYΝRAVSKFΝDQADRYIFAPAARGYRKVTP M M M h l M M M M M I M M M M I M M M I I I 1 1 1 I I I I I I I I 1 1 I I 1 1 1 1 = I m913 MKKTAYAFLLLIGFASAPAFAETRPADPYEGYΝRAVFKFΝDQADRYIFAPAARGYRKVAP 10 20 30 40 50 60 70 80 90 100 110 120 g913.pep KPVRAGVSΝFFΝΝLRDWSFGSΝILRLDIKRASEDLVRVGIΝTTFGLGGLIDIAGAGGVP 111 II I M II I II I II II II II II II 11 II 11 II I II 11 II I II II 11 II 111111 h I m913 KPVRAGVSΝFFΝΝLCDWSFGSΝILRLDIKRASEDLVRVGIΝTTFGLGGLIDIAGAGGIP 70 80 90 100 110 120 130 140 150 160 170 180 g913. pep DNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGITSVYPPKNIVFHTPAGRWGTT M M M M M M I M M M M M M M M M M I M M M I M 1 1 1 1 1 1 = I h 1 1 I I I I m913 DNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGITSVYSPKNIVFRTPVGRWGTT 130 140 150 160 170 180 190 200 210 220 230 240 g913.pep AAAAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRARQTGATPAEGTEDNIDIDIDEL h M M M M M M I M M I M M M M M M M M M M M M M M I M M M I I I m913 AVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRARQTGATPAEGTEDNIDI - -DEL 190 200 210 220 230 250 260 270 g913.pep VESAETGAAEPAVHEDSVSETQAEAAGEAETQPGTQPX m913 VESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQPX 240 250 260 270 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2769>: a913.seq 1 ATGAAAAAAA CCGCCTATGC CTTCCTCCTG CTGATCGGGT TCGCTTCCGC 51 CCCTGCATTT GCCGAAACCC GCCCCGCCGA CCCTTATGAA GGCTACAACC 101 GCGCCGTTTT CAAATTCAAC GACCAAGCCG ACCGCTACAT TTTCGCCCCT 151 GCCGCGCGCG GCTACCGCAA AGTTGCGCCG AAACCCGTCC GCGCCGGCGT 201 GTCCAATTTT TTTAACAACC TGTGCGACGT GGTCAGCTTC GGCAGCAATA 251 TCTTGCGCTT AGACATCAAA CGCGCAAGCG AAGACCTTGT CCGCGTCGGT 301 ATCAACACCA CTTTCGGTTT GGGCGGGCTT ATCGACATCG CCGGCGCGGG 351 CGGCATTCCC GACAATAAAA ACACCTTGGG CGACACGTTT GCTTCGTGGG 401 GATGGAAAAA CAGCAATTAT TTCGTGTTGC CCGTCTTAGG GCCGTCCACC 451 GTCCGCGACG CGCTCGGCAC GGGTATTACC TCCGTTTATT CGCCCAAGAA 501 TATCGTCTTC CGCACCCCTG TCGGACGCTG GGGCACGACT GCCGTATCCG 551 CCGTCAGTAC GCGCGAAGGC CTGCTCGATT TGACCGACAG TCTGGACGAA 601 GCCGCCATCG ACAAATACAG CTACACGCGC GACCTCTATA TGAAAGTCCG 651 TGCGCGGCAG ACCGGTGCAA CACCTGCCGA AGGTACGGAA GATAACATCG 701 ACATCGACGA ATTGGTCGAA AGTGCCGAAA CCGGCGCGGC GGAAACTGCC 751 GTTCAAGAAG ATTCCGTATC CGAAACACAG GCAGAAGCAG CAGGGGAAGC 801 CGAAACGCAA CCTGGAACAC AACCTGGAAC ACAACCTTAA This corresponds to the amino acid sequence <SEQ ID 2770; ORF 913.a>: a913.pep 1 MKKTAYAFLL LIGFASAPAF AETRPADPYE GYΝRAVFKFΝ DQADRYIFAP 51 AARGYRKVAP KPVRAGVSNF FNNLCDWSF GSNILRLDIK RASEDLVRVG 101 INTTFGLGGL IDIAGAGGIP DNKNTLGDTF ASWGWKNSNY FVLPVLGPST 151 VRDALGTGIT SVYSPKNIVF RTPVGRWGTT AVSAVSTREG LLDLTDSLDE 201 AAIDKYSYTR DLYMKVRARQ TGATPAEGTE DNIDIDELVE SAETGAAETA 251 VQEDSVSETQ AEAAGEAETQ PGTQPGTQP* m913/a913 100.0% identity in 275 aa overlap 10 20 30 40 50 60 m913. pep MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQADRYIFAPAARGYRKVAP II II II II I II II II II I II II I I II II I II II II II I II II II I II II II II I II I II I a913 MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQADRYIFAPAARGYRKVAP 10 20 30 40 50 60 70 80 90 100 110 120 m913.pep KPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGINTTFGLGGLIDIAGAGGIP II II II I II II II II II I II II II II II I I II II I II I II I II I I I II I I I I I I I II I I I a913 KPVRAGVSNFFNNLCDWSFGSNILRLDIKRASEDLVRVGINTTFGLGGLIDIAGAGGIP 70 80 90 100 110 120 130 140 150 160 170 180 m913. pep DNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGITSVYSPKNIVFRTPVGRWGTT II I II II II II I I II I II II II II II I I I II I II II II II II I I II I I II I II I II I I I I a913 DNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGITSVYSPKNIVFRTPVGRWGTT 130 140 150 160 170 180 190 200 210 220 230 240 m913. pep AVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRARQTGATPAEGTEDNIDIDELVE II II I I II I II II I II II II II I II II I II II II II I II I I I I I II I I II I I I II I I I II a913 AVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRARQTGATPAEGTEDNIDIDELVE 190 200 210 220 230 240 250 260 270 m913. pep SAETGAAETAVQEDSVSETQAEAAGEAETQPGTQPX II II II II I I I II I II I I I II II II II II I II I I I a913 SAETGAAETAVQEDSVSETQAEAAGEAETQPGTQPGTQPX 250 260 270 280 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2771>: g914. seq 1 ATGAAAAAAT GTATTTTGGG CATTTTGACC GCGTGTGCCG CCATGCCTGC 51 ATTTGCCGAC AGAATCAGCG ATTTGGAAGC ACGTCTGGCG CAGTTGGAAC 101 ACCGTGTCGC CGTATTGGAA AGCGGCGGCA ATACCGTCAA AATCGACCTT 151 TTCGGTTCAA ATTCCACCAT GTATGTATGC AGCGTTACGC CTTTTCAGAA 201 GacgtttGag gCAAGCGATC GGAATGAAGG CGTGGCGCGG CAGAAAGTGC 251 GTCAGGCGTG CAACCGCGAA ACTTCGGCAA TGTTTTGCGG AGATGAGGCA 301 ATCCGATGCA GAAAATTCGA TTGATGTATC GGTTGGACGG ATAAAGAAAC 351 GGATACGGAG CTTGGCTTCC GTCTCTGTTT TTCTCTGCCC GATTTTCCAT 401 GCATCGGGTT TCAGACGGCA TTGGAGTGTC AGTCGTGTTC TGCCGATTCG 451 taggctTCGA CGATTTTTTG CACCAGAGGA TGCCGGACAA CGTCTTCGCC 501 GGTGAAGGTA TGGAAATACA GTCCTGCCAC GCCGTGCAGT TTCTCACGTG 551 CGTCTTTCAA TCCCGATTTG ATGTTTTTGG GCAGGTcgaT TTGGCTGGTG 601 TCGCCGGTAA TGACGGCTTT CGCgccgaag ccGATGCGGG TCAGGAACAT 651 TTTCATTTGT TCGGGCGTGg tgTtttGcgC TTCGTCGAGG ATGATGTATG 701 CGCCGTTGAg cgTCCTGCCG CGCATATAG This corresponds to the amino acid sequence <SEQ ID 2772; ORF 914.ng>: g914.pep 1 MKKCILGILT ACAAMPAFAD RISDLEARLA QLEHRVAVLE SGGΝTVKIDL 51 FGSΝSTMYVC SVTPFQKTFE ASDRΝEGVAR QKVRQACΝRE TSAMFCGDEA 101 IRCRKFD*CI GWTDKETDTE LGFRLCFSLP DFPCIGFQTA LECQSCSADS 151 *ASTIFCTRG CRTTSSPVKV WKYSPATPCS FSRASFΝPDL MFLGRSIWLV 201 SPVMTAFAPK PMRVRΝIFIC SGWFCASSR MMYAPLSVLP RI* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2773>: m914.seq 1 ATGAAAAAAT GTATTTTGGG CATTTTGACC GCGTGTGCCG CCATGCCTGC 51 ATTTGCCGAC AGAATCGGCG ATTTGGAAGC ACGTCTGGCG CAGTTGGAAC 101 ACCGTGTCGC CGTATTGGAA AGCGGCGGCA ATACCGTCAA AATCGACCTT 151 TTCGGTTCAA ATTCCACCAT GTATGTATGC AGCGTTACGC CTTTTCAGAA 201 GACGTTTGAG GCAAGCGATC GGAATGAAGG CGTGGCGCGG CAGAAAGTGC 251 GTCAGGCGTG CAACCGCGAA ACTTCGGCAA TGTTTTGCGA AGATGAGGCA 301 ATCCGATGCA GAAAATTCGA TTGATGTATC GGTTGGACGG ATAAAGAAAC 351 GGATACGGAT ACGGAGCTTG GCTTCCGTAT CTGTTTTTCT CTGCCTGATT 401 TTCCATGCAT CGGGTTTCAG ACGGCATTGG AATGTCAGTC GTGTTCTGCC 451 GATTCGTAGG CTTCGACGAT TTTTTGCACC AAAGGATGCC GGACAACGTC 501 TTCGCCGGTA AAGGTGTGGA AATACAGCCC TTCCACGTTG TGCAGTTTCT 551 CACGCGCATC TTTTAATCCC GATTTGATGT TTTTGGGCAG GTCGATTTGG 601 CTGGTGTCGC CGGTAATGAC GGCTTTCGCG CCGAAGCCGA TGCGGGTCAG 651 GAACATTTTC ATTTGTTCGG GCGTGGTGTT TTGCGCTTCG TCGAGGATGA 701 TGTATGCGCC GTTGAGCGTC CTGCCGCGCA TATAG This corresponds to the amino acid sequence <SEQ ID 2774; ORF 914>: m914.pep 1 MKKCILGILT ACAAMPAFAD RIGDLEARLA QLEHRVAVLE SGGΝTVKIDL 51 FGSΝSTMYVC SVTPFQKTFE ASDRNEGVAR QKVRQACNRE TSAMFCEDEA 101 IRCRKFDXCI GWTDKETDTD TELGFRICFS LPDFPCIGFQ TALECQSCSA 151 DSXASTIFCT KGCRTTSSPV KVWKYSPSTL CSFSRASFNP DLMFLGRSIW 201 LVSPVMTAFA PKPMRVRNIF ICSGWFCAS SRMMYAPLSV LPRI* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 914 shows 96.7% identity over a 244 aa overlap with a predicted ORF (ORF 914.ng) from N gonorrhoeae: g914/m914 10 20 30 40 50 60 g914.pep MKKCILGILTACAAMPAFADRISDLEARLAQLEHRVAVLESGGΝTVKIDLFGSΝSTMYVC m914 MKKCI GILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGGΝTVKIDLFGSΝSTMYVC 10 20 30 40 50 60 70 80 90 100 110 119 g914. pep SVTPFQKTFEAΞDRΝEGVARQKVRQACΝRETSAMFCGDEAIRCRKFDXCIGWTDKETDT- 1 1 1 1 1 1 1 1 1 M 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 M 1 1 1 1 M M M I M M M I M M M M m914 SVTPFQKTFEASDRΝEGVARQKVRQACΝRETSAMFCEDEAIRCRKFDXCIGWTDKETDTD 70 80 90 100 110 120 120 130 140 150 160 170 g914.pep -ELGFRLCFSLPDFPCIGFQTALECQSCSADSXASTIFCTRGCRTTSSPVKVWKYSPATP m914 TELGFRICFSLPDFPCIGFQTALECQSCSADSXASTIFCTKGCRTTSSPVKVWKYSPSTL 130 140 150 160 170 180 180 190 200 210 220 230 g914.pep CSFSRASFΝPDLMFLGRSIWLVSPVMTAFAPKPMRVRΝIFICSGWFCASSRMMYAPLSV MMMMMIMMMMMMIMMMMMMMMMMMMMMMMMM 914 CSFSRASFΝPDLMFLGRSI LVSPVMTAFAPKPMRVRΝIFICSGWFCASSRMMYAPLSV 190 200 210 220 230 240 240 g914.pep LPRIX Mill m914 LPRIX The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2775>: a914.seq 1 ATGAAAAAAT GTATTTTGGG CATTTTGACC GCGTGTGCCG CCATGCCTGC 51 ATTTGCCGAC AGAATCGGCG ATTTGGAAGC ACGTCTGGCG CAGTTGGAAC 101 ACCGTGTCGC CGTATTGGAA AGCGGCAGCA ATACCGTCAA AATCGACCTT 151 TTCGGTTCAA ATTCCACCAT GTATGTATGC AGCGTTACGC CTTTTCAGAA 201 GACGTTTGAG GCAAGCGATC GGAATGAAGG CGTGGCGCGG CAGAAAGTGC 251 GTCAGGCGTG CAACCGCGAA ACTTCGGCAA TGTTTTGCGA AGATGAGGCA 301 ATCCGATGCA GAAAATTCGA TTGATGTATC GGTTGGACGG ATAAAGAAAC 351 GGATACGGAG CTTGGCTTCC GTATCTGTTT TTCTCTGCCC GATTTTCCAT 401 GCATCGGGTT TCAGACGGCA TTGGAATGTC AGTCGTGTTC TGCCGATTCG 451 TAGGCTTCGA CGATTTTTTG CACCAAAGGA TGCCGGACAA CGTCTTCGCC 501 GGTAAAGGTG TGGAAATACA GCCCTTCCAC GCCGTGCAGT TTCTCACGCG 551 CATCTTTTAA TCCCGATTTG ATGTTTTTGG GCAGGTCGAT TTGGCTGGTG 601 TCGCCGGTAA TGACGGCTTT CGCGCCGAAG CCGATGCGGG TCAGGAACAT 651 TTTCATTTGT TCGGGCGTGG TGTTTTGCGC TTCGTCGAGG ATGATGTATG 701 CGCCGTTGAG CGTCCTGCCG CGCATATAG This corresponds to the amino acid sequence <SEQ ID 2776; ORF 914.a>: a914.pep 1 MKKCILGILT ACAAMPAFAD RIGDLEARLA QLEHRVAVLE SGSΝTVKIDL 51 FGSΝSTMYVC SVTPFQKTFE ASDRΝEGVAR QKVRQACΝRE TSAMFCEDEA 101 IRCRKFD*CI GWTDKETDTE LGFRICFSLP DFPCIGFQTA LECQSCSADS 151 *ASTIFCTKG CRTTSSPVKV WKYSPSTPCS FSRASFΝPDL MFLGRSIWLV 201 SPVMTAFAPK PMRVRΝIFIC SGWFCASSR MMYAPLSVLP RI* m914/a914 98.4% i iddeennttiittyy i inn 224444 aaaa oovveerrllaapp 1 100 2200 30 40 50 60 m914 . pep MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGGNTVKIDLFGSNSTMYVC I I I II I II I I II II II I II II I II II I I II II II I I I I I I II : II II I II I I I I I II I II a914 MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGSNTVKIDLFGSNSTMYVC 10 20 30 40 50 60 70 80 90 100 110 120 m914.pep SVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIRCRKFDXCIGWTDKETDTD I II II I I II I II II I I II II II II II II I I II I I II I II II II II II I II II I I I II I a914 SVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIRCRKFDXCIGWTDKETD-- 70 80 90 100 110 130 140 150 160 170 180 m914.pep TELGFRICFSLPDFPCIGFQTALECQSCSADSXASTIFCTKGCRTTSSPVKVWKYSPSTL I II II I II I II II II I I II II I II I I I I I II II II II I II II I I I II I II I I I II II I I a914 TELGFRICFSLPDFPCIGFQTALECQSCSADSXASTIFCTKGCRTTSSPVKVWKYSPSTP 120 130 140 150 160 170 190 200 210 220 230 240 m914.pep CSFSRASFNPDLMFLGRSIWLVSPVMTAFAPKPMRVRNIFICSGWFCASSRMMYAPLΞV I I II II I II II II II II II II II I I II II I II II I II II II II II II I II II I II I I II I a914 CSFSRASFNPDLMFLGRSIWLVSPVMTAFAPKPMRVRNIFICSGVVFCASSRMMYAPLSV 180 190 200 210 220 230 m914.pep LPRIX IMM a914 LPRIX 240 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2777>: g915.seq 1 ATGAAGAAAA CCCTGTTGGC AATTGTTGCC gtTTTCGCCT TAAGTGCCTG 51 CCGGCaggcg gaAGaggcac cgccgCCTTT ACCCCGGCAG AtTAGCGacc 101 gttcggtcgg aCACTAttgC Agtatgaacc tgaccgaaca caacggcccc 151 aaagcccaga tttttttgaa CGGCAAACCC GATCAGCCCG TTTGGTTCTC 201 CACCGTcaag cagatgttcg GCTATACCAA GCTGCCCGAA GAGCCCAAAG 251 GCATCCGCGT GATTTACGTT ACCGATATGG GCAATGTTAC CGATTGGACG 301 AATCCTAATG CCGACACGGA GTGGATAGAT GCGAAAAAAG CCTTTTACGT 351 CATCGACAGC GGCTTTATCG GCGGTATGGG CGCGGAAGAC GCGCTGCCGT 401 TCGGCAACAA GGAGCAGGCT GAAAAATTTG CAAAGGATAA AGGCGGCAAG 451 GTCGTCGGTT TTGACGATAT GCCCGATGCT TACATTTTCA AGTAA This corresponds to the amino acid sequence <SEQ ID 2778; ORF 915.ng>: g915.pep 1 MKKTLLAIVA VFALSACRQA EEAPPPLPRQ ISDRSVGHYC SMΝLTEHΝGP 51 KAQIFLNGKP DQPVWFSTVK QMFGYTKLPE EPKGIRVIYV TDMGNVTDWT 101 NPNADTEWID AKKAFYVIDS GFIGGMGAED ALPFGNKEQA EKFAKDKGGK 151 WGFDDMPDA YIFK* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2779>: m915.seq 1 ATGAAAAAAA CCCTGTTGGC AATTGTTGCC GTTTCCGCCT TAAGTGC . tG 51 CCGGCAGGCG GAAGAGGGAC CGCCGCCTTT ACCCCGGCAG ATTAGCGACC 101 GTTCGGTCGG ACACTATTGC AGTATGAACC TGACCGAACA CAACGGCCCC 151 AAAGCCCAGA TTTTCTTGAA CGGCAAACCC GATCAGCCCG TtTGGTTCTC 201 CACCATCAAG CAGATGTTCG GCTATACCAA GCTGCCCGAA GAGCCTAAAG 251 GCATCCGCGT GATTTACGTT ACCGATATGG GCAATGTTAC CGATTGGACG 301 AATCCCAATG CCGACACGGA GTGGATGGAT GCGAAAAAAG CCTTTTACGT 351 CATCGACAGC GGCTTTATCG GCGGTATGGG TGCGGAAGAC GCGCTGCCGT 401 TCGGCAACAA AGAGCAGGCT GAGAAATTTG CAAAGGATAA AGGCGGTAAG 451 GTTGTCGGTT TCGACGATAT GCCTGATACC TATATTTTCA AATAA This corresponds to the amino acid sequence <SEQ ID 2780; ORF 915>: m915.pep 1 MKKTLLAIVA VSALSXCRQA EEGPPPLPRQ ISDRSVGHYC SMNLTEHNGP 51 KAQIFLNGKP DQPVWFSTIK QMFGYTKLPE EPKGIRVIYV TDMGNVTDWT 101 NPNADTEWMD AKKAFYVIDS GFIGGMGAED ALPFGNKEQA EKFAKDKGGK 151 VVGFDDMPDT YIFK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 915 shows 97.0% identity over a 164 aa overlap with a predicted ORF (ORF 915.ng) from N gonorrhoeae: m915/g915 10 20 30 40 50 60 m915. pep MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMΝLTEHΝGPKAQIFLΝGKP 1 1 1 1 I I I ! 1 1 1 M M M M I h l M M M I M M M M M M M M M M I M M M M g915 MKKTLLAIVAVFALSACRQAEEAPPPLPRQISDRSVGHYCSMΝLTEHΝGPKAQIFLΝGKP 10 20 30 40 50 60 70 80 90 100 110 120 m915.pep DQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGΝVTDWTΝPΝADTEWMDAKKAFYVIDS 11 M 1111 = 111111 II 1111111111111111 II 111 M I M 11111 = 11 II 11 M M I g915 DQPVWFSTVKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNPNADTEWIDAKKAFYVIDS 70 80 90 100 110 120 130 140 150 160 m915.pep GFIGGMGAEDALPFGNKEQAEKFAKDKGGKWGFDDMPDTYIFKX MMMMMMMMMMMMMMMMMIMIhlMM g915 GFIGGMGAEDALPFGNKEQAEKFAKDKGGKWGFDDMPDAYIFKX 130 140 150 160 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2781>: a915.seq 1 ATGAAAAAAA CCCTGTTGGC AATTGTTGCC GTTTCCGCCT TAAGTGCCTG 51 CCGGCAGGCG GAAGAGGGAC CGCCGCCTTT ACCCCGGCAG ATTAGCGACC 101 GTTCGGTCGG ACACTATTGC AGTATGAACC TGACCGAACA CAACGGCCCC 151 AAAGCCCAGA TTTTCTTGAA CGGCAAACCC GATCAGCCCG TTTGGTTCTC 201 CACCATCAAG CAGATGTTCG GCTATACCAA GCTGCCCGAA GAGCCTAAAG 251 GCATCCGCGT GATTTACGTT ACCGATATGG GCAATGTTAC CGATTGGACG 301 AATCCCAATG CCGACACGGA GTGGATGGAT GCGAAAAAAG CCTTTTACGT 351 CATCGACAGC GGCTTTATCG GCGGTATGGG TGCGGAAGAC GCGCTGCCGT 401 TCGGCAACAA AGAGCAGGCT GAGAAATTTG CAAAGGATAA AGGCGGTAAG 451 GTTGTCGGTT TCGACGATAT GCCTGATACC TATATTTTCA AATAA This corresponds to the amino acid sequence <SEQ ID 2782; ORF 915.a>: a915.pep 1 MKKTLLAIVA VSALSACRQA EEGPPPLPRQ ISDRSVGHYC SMΝLTEHΝGP 51 KAQIFLNGKP DQPVWFSTIK QMFGYTKLPE EPKGIRVIYV TDMGNVTDWT 101 NPNADTEWMD AKKAFYVIDS GFIGGMGAED ALPFGNKEQA EKFAKDKGGK 151 VVGFDDMPDT YIFK* m915/a915 99.4% identity in 164 aa overlap 10 20 30 40 50 60 m915. pep MKKTLLAIVAVSALSXCRQAEEGPPPLPRQISDRSVGHYCSMNLTEHNGPKAQIFLNGKP M M M M I M M M I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a915 MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNLTEHNGPKAQIFLNGKP 10 20 30 40 50 60 70 80 90 100 110 120 m915. pep DQPVWFSTIK Q MFGYTKLPEEPKGIRVIYVTDMGNVTDWTNPNADTEWMDAKKAFYVIDS MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a915 DQPVWFSTIK Q MFGYTKLPEEPKGIRVIYVTDMGNVTDWTNPNADTEWMDAKKAFYVIDS 70 80 90 100 110 120 130 140 150 160 m915. pep GFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDTYIFKX II I II II I II II I II II I I I II II II I II II II I II II II II II I a915 GFIGGMGAEDALPFGNKEQAEKFAKDKGGKWGFDDMPDTYIFKX 130 140 150 160 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2783>: g917.seq 1 ATGGTCAAac atctgccacT cgcCGTCctg actgctTtgc tgcttgcagc 51 gtgcGGCGGT Tcggacaaac cgcctgccga Aaaaccggca ccggcgGaAA 101 accaaAacgt atTgaAAATT TataACTGGT CGGAATACGT CGATCCGGAA 151 ACCGTTGCCG ATTTTGAAAA GAAAAACGGC ATCAAGGTTA CTTATGATGT 201 GTACGACAGT GATGAAACGC TGGAAAGCAA GGTGCTGACC GGAAAATCCG 251 GTTACGACAT TGTCGCGCCG TCCAATGCGT TTGTGGGCAG GCAGATTAAG 301 GCAGGTGCGT ATCAGAAAAT CGATAAGTCG ATGATTCCCA ATTATAAACA 351 TCTCAACCCT GAAATGATGA GGCTGATGGA CGGGGTCGAT CCCGACCACG 401 AATACGCCGT GCCGTTTTAT TGGGGGACAA ATACCTTCGC CATCAATACC 451 GAACGCGTGA AAAAGGCTTT GGGTACGGAC AAGCTGCCGG ACAACCAGTG 501 GGATTTGGTG TTCAACCCCG AATACACGTT CAAACTCAAA CAATGCGGCA 551 TCAGCTATTT GGACAGCGCG GCGGAAATTT ATCCCATGGT GTTGAACTAT 601 TTGGGCAAAA ACCCGAACAG CAGCAATACG GAAGACATCA GGGAGGCAAC 651 CGCCCTGCTC AAGAAAAACC GCCCCAATAT CAAACGCTTT ACTTCGTCCG 701 GCTTTATCGA TGATTTGGCG CGCGGCGATA CCTGCGTAAC AATCGGTTTC 751 GGCGGAGATT TGAACATCGC CAAACGCCGT GCCGAAGAAG CGGGCGGCAA 801 GGAAAAAATC CGCGTGATGA TGCCGAAAGA GGGCGTGGGG ATTTGGGTGG 851 ATTCTTTCGT GATTCCGAAA GATGCGAAAA ACGTCGCCAA CGCGCACAAA 901 TACATCAACG ACTTCCTCGA TCCGGAAGTG TCGGCGAAAA ACGGCAATTT 951 cgttacCTAC GCGCCTTCGA GCAAGCCGGC GCGCGATTTG ATGGAGGACG 1001 AATTTAAAAA CGACAATACG ATTTTCCCGA GCGGGGAAGA TTTGAAAAAC 1051 AGCTTTATCA TGGTGCCTAT CCGGCCGGCG GCATTGAAGT TTATGGTGCG 1101 CCAGTGGCAG GATGTGAAGG CGGGGAAATA A This corresponds to the amino acid sequence <SEQ ID 2784; ORF 917.ng>: g917.pep 1 MVKHLPLAVL TALLLAACGG SDKPPAEKPA PAEΝQΝVLKI YΝWSEYVDPE 51 TVADFEKKΝG IKVTYDVYDS DETLESKVLT GKSGYDIVAP SΝAFVGRQIK 101 AGAYQKIDKS MIPΝYKHLΝP EMMRLMDGVD PDHEYAVPFY WGTΝTFAIΝT 151 ERVKKALGTD KLPDΝQWDLV FΝPEYTFKLK QCGISYLDSA AEIYPMVLΝY 201 LGKΝPΝSSΝT EDIREATALL KKΝRPΝIKRF TSSGFIDDLA RGDTCVTIGF 251 GGDLΝIAKRR AEEAGGKEKI RVMMPKEGVG IWVDSFVIPK DAKΝVAΝAHK 301 YIΝDFLDPEV SAKΝGΝFVTY APSSKPARDL MEDEFKΝDΝT IFPSGEDLKΝ 351 SFIMVPIRPA ALKFMVRQWQ DVKAGK* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2785>: m917. seq 1 ATGACCAAAC ATCTGCCCCT GGCCGTCCTG ACTGCTTTGC TGCTTGCAGC 51 GTGCGGCGGT TCGGACAAAC CGCCTGCCGA AAAACCGGCA CCGGCGGAAA 101 ACCAAAACGT ATTGAAAATT TACAACTGGT CGGAATATGT CGATCCGGAA 151 ACCGTTGCCG ATTTTGAAAA GAAAAACGGC ATCAAGGTTA CTTATGATGT 201 GTACGACAGC GATGAAACGC TGGAAAGCAA GGTGCTGACA GGCAAGTCCG 251 GTTACGACAT TGTCGCGCCG TCCAATGCGT TTGTGGGCAG GCAGATTAAG 301 GCAGGTGCGT ATCAGAAAAT CGATAAGTCG CTGATTCCCA ATTATAAACA 351 CCTCAACCCC GAAATGATGA GGCTGATGGA CGGGGTCGAT CCCGGCCACG 401 AATACGCCGT GCCGTTTTAT TGGGGGACAA ATACCTTCGC CATCAATACC 451 GAACGCGTGA AAAAGGCTTT GGGTACGGAC AAGCTGCCGG ACAACCAGTG 501 GGATTTGGTG TTCGACCCCG AATACACGTC CAAACTCAAG CAATGCGGCA 551 TCAGCTATTT GGACAGCGCG GCGGAAATCT ATCCTATGGT GTTGAACTAT 601 TTGGGTAAAA ACCCGAACAG CAGCAATACG GAAGACATCA GGGAGGCAAC 651 CGCCCTACTC AAGAAAAACC GCCCCAATAT CAAACGCTTT ACTTCGTCCG 701 GCTTTATCGA TGATTTGGCG CGCGGCGATA CCTGCGTAAC AATCGGTTTC 751 GGCGGCGATT TGAACATCGC CAAACGCCGT GCCGAAGAAG CGGGCGGCAA 801 GGAAAAAATC CGCGTGATGA TGCCCAAAGA GGGCGTGGGG ATTTGGGTGG 851 ATTCTTTCGT GATTCCGAAA GATGCGAAAA ACGTCGCCAA CGCGCACAAA 901 TACATCAACG ACTTCCTCGA CCCGGAAGTG TCGGCGAAAA ACGGCAATTT 951 CGTTACTTAC GCGCCTTCGA GCAAGCCTGC GCGTGAGCTG ATGGAAGACG 1001 AATTTAAAAA CGACAATACG ATTTTCCCAA CCGAGGAGGA TTTGAAAAAC 1051 AGCTTTATCA TGGTGCCTAT CCAGCCGGCG GCATTGAAGT TTATGGTGCG 1101 CCAGTGGCAG GATGTGAAGG CGGGGAAATA A This corresponds to the amino acid sequence <SEQ ID 2786; ORF 917>: m917.pep 1 MTKHLPLAVL TALLLAACGG SDKPPAEKPA PAENQNVLKI YNWSEYVDPE 51 TVADFEKKNG IKVTYDVYDS DETLESKVLT GKSGYDIVAP SNAFVGRQIK 101 AGAYQKIDKS LIPNYKHLNP EMMRLMDGVD PGHEYAVPFY WGTNTFAINT 151 ERVKKALGTD KLPDNQWDLV FDPEYTSKLK QCGISYLDSA AEIYPMVLNY 201 LGKNPNSSNT EDIREATALL KKNRPNIKRF TSSGFIDDLA RGDTCVTIGF 251 GGDLNIAKRR AEEAGGKEKI RVMMPKEGVG IWVDSFVIPK DAKNVANAHK 301 YINDFLDPEV SAKNGNFVTY APSSKPAREL MEDEFKNDNT IFPTEEDLKN 351 SFIMVPIQPA ALKFMVRQWQ DVKAGK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 917 shows 97.6% identity over a 376 aa overlap with a predicted ORF (ORF 917.ng) from N. gonorrhoeae: m917/g917 10 20 30 40 50 60 m917. ep MTKHLPLAVLTALLLAACGGSDKPPAEKPAPAEΝQΝVLKIYΝWSEYVDPETVADFEKKΝG hMMMMMMMMMMMMIIMMIMMMMMMMIMIMMIMM g917 MVKHLPLAVLTALLLAACGGSDKPPAEKPAPAEΝQΝVLKIYΝWSEYVDPETVADFEKKΝG 10 20 30 40 50 60 70 80 90 100 110 120 m917.pep IKVTYDVYDSDETLESKVLTGKSGYDIVAPSΝAFVGRQIKAGAYQKIDKSLIPΝYKHLΝP g917 IKVTYDVYDSDETLESKVLTGKSGYDIVAPSΝAFVGRQIKAGAYQKIDKSMIPΝYKHLΝP 70 80 90 100 110 120 130 140 150 160 170 180 m917. ep EMMRLMDGVDPGHEYAVPFYWGTΝTFAIΝTERVKKALGTDKLPDΝQWDLVFDPEYTSKLK MMMMMMMMMMMMMMMMMMMhllM III g917 EMMRLMDGVDPDHEYAVPFYWGTΝTFAIΝTERVKKALGTDKLPDΝQWDLVFΝPEYTFKLK 130 140 150 160 170 180 190 200 210 220 230 240 m917. pep QCGISYLDSAAEIYPMVLΝYLGKΝPΝSSΝTEDIREATALLKKΝRPΝIKRFTSSGFIDDLA MMMMMMMIMMIMMMIIMIMMMMMMMMMIMMMMM g917 QCGISYLDSAAEIYPMVLΝYLGKΝPΝSSΝTEDIREATALLKKΝRPΝIKRFTSSGFIDDLA 190 200 210 220 230 240 250 260 270 280 290 300 m917. pep RGDTCVTIGFGGDLΝIAKRRAEEAGGKEKIRVMMPKEGVGIWVDSFVIPKDAKΝVAΝAHK I II I II 111111111111 M I II 11 II 11111111 M 111111111 II I M 1111 M I M g917 RGDTCVTIGFGGDLΝIAKRRAEEAGGKEKIRVMMPKEGVGIWVDSFVIPKDAKΝVAΝAHK 250 260 270 280 290 300 310 320 330 340 350 360 m917.pep YIΝDFLDPEVSAKΝGΝFVTYAPSSKPARELMEDEFKΝDΝTIFPTEEDLKΝSFIMVPIQPA i 11111111111 = 11 g917 YIΝDFLDPEVSAKΝGΝFVTYAPSSKPARDLMEDEFKΝDΝTIFPSGEDLKΝSFIMVPIRPA 310 320 330 340 350 360 370 m917. pep ALKFMVRQWQDVKAGKX g917 ALKFMVRQWQDVKAGKX 370 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2787>: a917.seq 1 ATGACCAAAC ATCTGCCCCT GGCCGTCCTG ACTGCTTTGC TGCTTGCAGC 51 GTGCGGCGGT TCGGACAAAC CGCCTGCCGA AAAACCGGCG CCGGCGGAAA 101 ACCGAAACGT ATTGAAAATT TACAACTGGT CGGAATACGT CGATCCGGAA 151 ACCGTTGCCG ATTTTGAAAA GAAAAACGGC ATCAAGGTTA CTTATGATGT 201 GTACGACAGC GATGAAACGC TGGAAAGCAA GGTGCTGACC GGAAAATCTG 251 GTTACGACAT TGTCGCGCCG TCCAATGCGT TTGTGGGCAG GCAGATTAAG 301 GCAGGTGCGT ATCAGAAAAT CGATAAGTCG CTGATTCCCA ATTATAAACA 351 CCTCAACCCC GAAATGATGA GGCTGATGGA CGGGGTCGAT CCCGGCCACG 401 AATACGCCGT GCCGTTTTAT TGGGGGACAA ATACCTTCGC CATCAATACC 451 GAACGCGTGA AAAAGGCTTT GGGTACGGAC AAGCTGCCGG ACAACCAGTG 501 GGATTTGGTG TTCGACCCCG AATACACGTC CAAACTCAAG CAATGCGGCA 551 TCAGCTATTT GGACAGCGCG GCGGAAATCT ATCCTATGGT GTTGAACTAT 601 TTGGGTAAAA ACCCGAACAG CAGCAATACG GAAGACATCA GGGAGGCAAC 651 CGCCCTACTC AAGAAAAACC GCCCCAATAT CAAACGCTTT ACTTCGTCCG 701 GCTTTATCGA TGATTTGGCG CGCGGCGATA CCTGCGTAAC AATCGGTTTC 751 GGCGGCGATT TGAACATCGC CAAACGCCGT GCCGAAGAAG CGGGCGGCAA 801 GGAAAAAATC CGCGTGATGA TGCCCAAAGA GGGCGTGGGG ATTTGGGTGG 851 ATTCTTTCGT GATTCCGAAA GATGCGAAAA ACGTCGCCAA CGCGCACAAA 901 TACATCAACG ACTTCCTCGA CCCGGAAGTG TCGGCGAAAA ACGGCAATTT 951 CGTTACTTAC GCGCCTTCGA GCAAGCCTGC GCGTGAGCTG ATGGAAGACG 1001 AATTTAAAAA CGACAATACG ATTTTCCCAA CCGAGGAGGA TTTGAAAAAC 1051 AGCTTTATCA TGGTGCCTAT CCAGCCGGCG GCATTGAAGT TTATGGTGCG 1101 CCAGTGGCAG GATGTGAAGG CGGGGAAATA A This corresponds to the amino acid sequence <SEQ ID 2788; ORF 917.a>: a917.pep 1 MTKHLPLAVL TALLLAACGG SDKPPAEKPA PAEΝRΝVLKI YΝWSEYVDPE 51 TVADFEKKΝG IKVTYDVYDS DETLESKVLT GKSGYDIVAP SΝAFVGRQIK 101 AGAYQKIDKS LIPΝYKHLΝP EMMRLMDGVD PGHEYAVPFY WGTΝTFAIΝT 151 ERVKKALGTD KLPDΝQWDLV FDPEYTSKLK QCGISYLDSA AEIYPMVLΝY 201 LGKΝPΝSSΝT EDIREATALL KKΝRPΝIKRF TSSGFIDDLA RGDTCVTIGF 251 GGDLΝIAKRR AEEAGGKEKI RVMMPKEGVG IWVDSFVIPK DAKΝVAΝAHK 301 YIΝDFLDPEV SAKΝGΝFVTY APSSKPAREL MEDEFKΝDΝT IFPTEEDLKΝ 351 SFIMVPIQPA ALKFMVRQWQ DVKAGK* m917/a917 99.7% identity in 376 aa overlap 10 20 30 40 50 60 m917. pep MTKHLPLAVLTALLLAACGGSDKPPAEKPAPAEΝQΝVLKIYΝWSEYVDPETVADFEKKΝG I I II II II I II I I II II II I II I II II II II I II: I I II II II II II II II I II II II II a917 MTKHLPLAVLTALLLAACGGSDKPPAEKPAPAEΝRΝVLKIYΝWSEYVDPETVADFEKKΝG 10 20 30 40 50 60 70 80 90 100 110 120 m917.pep IKVTYDVYDSDETLESKVLTGKSGYDIVAPSΝAFVGRQIKAGAYQKIDKSLIPΝYKHLΝP II I I II II I II II II II II I II I II I I I I II II II I I I II I II I II II I II II II II I II a917 IKVTYDVYDSDETLESKVLTGKSGYDIVAPSΝAFVGRQIKAGAYQKIDKSLIPΝYKHLΝP 70 80 90 100 110 120 130 140 150 160 170 180 m917.pep EMMRLMDGVDPGHEYAVPFYWGTΝTFAIΝTERVKKALGTDKLPDΝQWDLVFDPEYTSKLK I II I II I II I II II I I II II I II II I II II I II II II II I II II II II II II I II I II II a917 EMMRLMDGVDPGHEYAVPFYWGTΝTFAIΝTERVKKALGTDKLPDΝQWDLVFDPEYTSKLK 130 140 150 160 170 180 190 200 210 220 230 240 m917.pep QCGISYLDSAAEIYPMVLΝYLGKΝPΝSSΝTEDIREATALLKKΝRPΝIKRFTSSGFIDDLA II II I II I II II I II II I I I II I II I I I II I II I I I II I II I I II I I I I II I I I I I II I I a917 QCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATALLKKNRPNIKRFTSSGFIDDLA 190 200 210 220 230 240 250 260 270 280 290 300 m917. pep RGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPKEGVGIWVDSFVIPKDAKNVANAHK MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a917 RGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPKEGVGIWVDSFVIPKDAKNVANAHK 250 260 270 280 290 300 310 320 330 340 350 360 m917. pep YINDFLDPEVSAKNGNFVTYAPSSKPARELMEDEFKNDNTIFPTEEDLKNSFIMVPIQPA II I II II II II I II II I II II II I II II II II I I I I I I II II II I I I II I I II II II II I a917 YINDFLDPEVSAKNGNFVTYAPSSKPARELMEDEFKNDNTIFPTEEDLKNSFIMVPIQPA 310 320 330 340 350 360 370 m91 .pep ALKFMVRQWQDVKAGKX I II II II I I II I II I I I a917 ALKFMVRQWQDVKAGKX 370 The following partial DNA sequence was identified in N.gonorrhoeae <SEQ ID 2789>: g919.seq 1 ATGAAAAAAC ACCTGCTCCG CTCCGCCCTG TACGGcatCG CCGCCgccAT 51 CctcgCCGCC TGCCAAAgca gGAGCATCCA AACCTTTCCG CAACCCGACA 101 CATCCGTCAT CAACGGCCCG GACCGGCCGG CCGGCATCCC CGACCCCGCC 151 GGAACGACGG TTGCCGGCGG CGGGGCCGTC TATACCGTTG TGCCGCACCT 201 GTCCATGCCC CACTGGGCGG CGCaggATTT TGCCAAAAGC CTGCAATCCT 251 TCCGCCTCGG CTGCGCCAAT TTGAAAAACC GCCAAGGCTG GCAGGATGTG 301 TGCGCCCAAG CCTTTCAAAC CCCCGTGCAT TCCTTTCAGG CAAAGcGgTT 351 TTTTGAACGC TATTTCACGC cgtGGCaggt tgcaggcaAC GGAAGcCTTG 401 Caggtacggt TACCGGCTAT TACGAACCGG TGCTGAAGGG CGACGGCAGG 451 CGGACGGAAC GGGCCCGCTT CCCGATTTAC GGTATTCCCG ACGATTTTAT 501 CTCCGTCCCG CTGCCTGCCG GTTTGCGGGG CGGAAAAAAC CTTGTCCGCA 551 TCAGGCAGac ggGGAAAAAC AGCGGCACGA TCGACAATGC CGGCGGCACG 601 CATACCGCCG ACCTCTCCCG ATTCCCCATC ACCGCGCGCA CAACGGcaat 651 caaaGGCAGG TTTGAaggAA GCCGCTTCCT CCCTTACCAC ACGCGCAACC 701 AAAtcaacGG CGGCgcgcTT GACGGCAAag cccCCATCCT CggttacgcC 751 GAagaccCcG tcgaacttTT TTTCATGCAC AtcoaaggCT CGGGCCGCCT 801 GAAAACCCcg tccggcaaat acatCCGCAt cggaTacgcc gacAAAAACG 851 AACAtccgTa tgtttccatc ggACGctaTA TGGCGGACAA AGGCTACCTC 901 AAGctcgggc agACCTCGAT GCAGGgoatσ aaagcCTATA TGCGGCAAAA 951 TCCGCAACGC CTCGCCGAAG TTTTGGGTCA AAACCCCAGC TATATCTTTT 1001 TCCGCGAGCT TGCCGGAAGC GGCAATGAGG GCCCCGTCGG CGCACTGGGC 1051 ACGCCACTGA TGGGGGAATA CGCCGGCGCA ATCGACCGGC ACTACATTAC 1X01 CTTGGGCGCG CCCTTATTTG TCGCCACCGC CCATCCGGTT ACCCGCAAAG 1151 CCCTCAACCG CCTGATTATG GCGCAGGATA CAGGCAGCGC GATCAAAGGC 1201 GCGGTGCGCG TGGATTATTT TTGGGGTTAC GGCGACGAAG CCGGCGAACT 1251 TGCCGGCAAA CAGAAAACCA CGGGATACGT CTGGCAGCTC CTGCCCAACG 1301 GCATGAAGCC CGAATACCGC CCGTGA This corresponds to the amino acid sequence <SEQ ID 2790; ORF 919.ng>: g919 .pep 1 MKKHLLRSAL YGIAAAILAA CQSRSIQTFP QPDTSVINGP DRPAGIPDPA 51 GTTVAGGGAV YTWPHLSMP HWAAQDFAKS LQSFRLGCAN LKNRQGWQDV 101 CAQAFQTPVH SFQAKRFFER YFTPWQVAGN GSLAGTVTGY YEPVLKGDGR 151 RTERARFPIY GIPDDFISVP LPAGLRGGKN LVRIRQTGKN SGTIDNAGGT 201 HTADLSRFPI TARTTAIKGR FEGSRFLPYH TRNQINGGAL DGKAPILGYA 251 EDPVELFFMH IQGSGRLKTP SGKYIRIGYA DKNEHPYVSI GRYMADKGYL 301 KLGQTSMQGI KAYMRQNPQR LAEVLGQNPS YIFFRELAGS GNEGPVGALG 351 TPLMGEYAGA IDRHYITLGA PLFVATAHPV TRKALNRLIM AQDTGSAIKG 401 AVRVDYFWGY GDEAGELAGK QKTTGYVWQL LPNGMKPEYR P* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2791>: m919.seq 1 ATGAAAAAAT ACCTATTCCG CGCCGCCCTG TACGGCATCG CCGCCGCCAT 51 CCTCGCCGCC TGCCAAAGCA AGAGCATCCA AACCTTTCCG CAACCCGACA 101 CATCCGTCAT CAACGGCCCG GACCGGCCGG TCGGCATCCC CGACCCCGCC 151 GGAACGACGG TCGGCGGCGG CGGGGCCGTC TATACCGTTG TACCGCACCT 201 GTCCCTGCCC CACTGGGCGG CGCAGGATTT CGCCAAAAGC CTGCAATCCT 251 TCCGCCTCGG CTGCGCCAAT TTGAAAAACC GCCAAGGCTG GCAGGATGTG 301 TGCGCCCAAG CCTTTCAAAC CCCCGTCCAT TCCTTTCAGG CAAAACAGTT 351 TTTTGAACGC TATTTCACGC CGTGGCAGGT TGCAGGCAAC GGAAGCCTTG 401 CCGGTACGGT TACCGGCTAT TACGAACCGG TGCTGAAGGG CGACGACAGG 451 CGGACGGCAC AAGCCCGCTT CCCGATTTAC GGTATTCCCG ACGATTTTAT 501 CTCCGTCCCC CTGCCTGCCG GTTTGCGGAG CGGAAAAGCC CTTGTCCGCA 551 TCAGGCAGAC GGGAAAAAAC AGCGGCACAA TCGACAATAC CGGCGGCACA 601 CATACCGCCG ACCTCTCCcG ATTCCCCATC ACCGCGCGCA CAACAGCAAT 651 CAAAGGCAGG TTTGAAGGAA GCCGCTTCCT CCCCTACCAC ACGCGCAACC 701 AAATCAACGG CGGCGCGCTT GACGGCAAAG CCCCGATACT CGGTTACGCC 751 GAAGACCCTG TCGAACTTTT TTTTATGCAC ATCCAAGGCT CGGGCCGTCT 801 GAAAACCCCG TCCGGCAAAT ACATCCGCAT CGGCTATGCC GACAAAAACG 851 AACATCCyTA CGTTTCCATC GGACGCTATA TGGCGGATAA GGGCTACCTC 901 AAACTCGGAC AAACCTCCAT GCAGGGCATT AAGTCTTATA TGCGGCAAAA 951 TCCGCAACGC CTCGCCGAAG TTTTGGGTCA AAACCCCAGC TATATCTTTT 1001 TCCGCGAGCT TGCCGGAAGC AGCAATGACG GCCCTGTCGG CGCACTGGGC 1051 ACGCCGCTGA TGGGGGAATA TGCCGGCGCA GTCGACCGGC ACTACATTAC 1101 CTTGGGTGCG CCCTTATTTG TCGCCACCGC CCATCCGGTT ACCCGCAAAG 1151 CCCTCAACCG CCTGATTATG GCGCAGGATA CCGGCAGCGC GATTAAAGGC 1201 GCGGTGCGCG TGGATTATTT TTGGGGATAC GGCGACGAAG CCGGCGAACT 1251 TGCCGGCAAA CAGAAAACCA CGGGATATGT CTGGCAGCTC CTACCCAACG GTATGAAGCC CGAATACCGc CCGTAA This corresponds to the amino acid sequence <SEQ ID 2792; ORF 919>: m919.pep 1 MKKYLFRAAL YGIAAAILAA CQSKSIQTFP QPDTSVINGP DRPVGIPDPA 51 GTTVGGGGAV YTWPHLSLP HWAAQDFAKS LQSFRLGCAN LKNRQGWQDV 101 CAQAFQTPVH SFQAKQFFER YFTPWQVAGN GSLAGTVTGY YEPVLKGDDR 151 RTAQARFPIY GIPDDFISVP LPAGLRSGKA LVRIRQTGKN SGTIDNTGGT 201 HTADLSRFPI TARTTAIKGR FEGSRFLPYH TRNQINGGAL DGKAPILGYA 251 EDPVELFFMH IQGSGRLKTP SGKYIRIGYA DKNEHPYVSI GRYMADKGYL 301 KLGQTSMQGI KSYMRQNPQR LAEVLGQNPS YIFFRELAGS SNDGPVGALG 351 TPLMGEYAGA VDRHYITLGA PLFVATAHPV TRKALNRLIM AQDTGSAIKG 401 AVRVDYFWGY GDEAGELAGK QKTTGYVWQL LPNGMKPEYR P* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N. gonorrhoeae ORF 919 shows 95.9 % identity over a 441 aa overlap with a predicted ORF (ORF 919. ng) from N. gonorrhoeae : m919/g919 10 20 30 40 50 60 m919 . pep MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPDPAGTTVGGGGAV I I h h h l l l l l l l l l l l l l l h l l l l I I I I I M M M I I I h l l l M i l l I h i l l l l g919 MKKHLLRSALYGIAAAILAACQSRSIQTFPQPDTSVINGPDRPAGIPDPAGTTVAGGGAV 10 20 30 40 50 60 70 80 90 100 110 120 m919.pep YTWPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER g919 YTWPHLSMPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKRFFER 70 80 90 100 110 120 130 140 150 160 170 180 m919. ep YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA I M I II I II I II M III II 11 Ml I II I III II 11111 II 11 II II 11 II I h 11 g919 YFTPWQVAGNGSLAGTVTGYYEPVLKGDGRRTERARFPIYGIPDDFISVPLPAGLRGGKN 130 140 150 160 170 180 190 200 210 220 230 240 m919.pep LVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL I I M I I I M I I l l l l I I M M M M M M M M M g919 LVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL 190 200 210 220 230 240 250 260 270 280 290 300 m919.pep DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL MMMMIMMMMMMMMMMMMMMMMMMMMIMIMMIM g919 DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL 250 260 270 280 290 300 310 320 330 340 350 360 m919.pep KLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFFRELAGSSNDGPVGALGTPLMGEYAGA g919 KLGQTSMQGIKAYMRQNPQRLAEVLGQNPSYIFFRELAGSGNEGPVGALGTPLMGEYAGA 310 320 330 340 350 360 370 380 390 400 410 420 m919.pep VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK M M M I M M M I M M I M M M M M M M M M I M M I M M I I M M I M M M g919 IDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK 370 380 390 400 410 420 430 440 m919.pep QKTTGYVWQLLPNGMKPEYRPX g919 QKTTGYVWQLLPNGMKPEYRPX 430 440 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2793>: a919.seq 1 ATGAAAAAAT ACCTATTCCG CGCCGCCCTG TGCGGCATCG CCGCCGCCAT 51 CCTCGCCGCC TGCCAAAGCA AGAGCATCCA AACCTTTCCG CAACCCGACA 101 CATCCGTCAT CAACGGCCCG GACCGGCCGG TCGGCATCCC CGACCCCGCC 151 GGAACGACGG TCGGCGGCGG CGGGGCCGTT TATACCGTTG TGCCGCACCT 201 GTCCCTGCCC CACTGGGCGG CGCAGGATTT CGCCAAAAGC CTGCAATCCT 251 TCCGCCTCGG CTGCGCCAAT TTGAAAAACC GCCAAGGCTG GCAGGATGTG 301 TGCGCCCAAG CCTTTCAAAC CCCCGTCCAT TCCGTTCAGG CAAAACAGTT 351 TTTTGAACGC TATTTCACGC CGTGGCAGGT TGCAGGCAAC GGAAGCCTTG 401 CCGGTACGGT TACCGGCTAT TACGAGCCGG TGCTGAAGGG CGACGACAGG 451 CGGACGGCAC AAGCCCGCTT CCCGATTTAC GGTATTCCCG ACGATTTTAT 501 CTCCGTCCCC CTGCCTGCCG GTTTGCGGAG CGGAAAAGCC CTTGTCCGCA 551 TCAGGCAGAC GGGAAAAAAC AGCGGCACAA TCGACAATAC CGGCGGCACA 601 CATACCGCCG ACCTCTCCCA ATTCCCCATC ACTGCGCGCA CAACGGCAAT 651 CAAAGGCAGG TTTGAAGGAA GCCGCTTCCT CCCCTACCAC ACGCGCAACC 701 AAATCAACGG CGGCGCGCTT GACGGCAAAG CCCCGATACT CGGTTACGCC 751 GAAGACCCCG TCGAACTTTT TTTTATGCAC ATCCAAGGCT CGGGCCGTCT 801 GAAAACCCCG TCCGGCAAAT ACATCCGCAT CGGCTATGCC GACAAAAACG 851 AACATCCCTA CGTTTCCATC GGACGCTATA TGGCGGACAA AGGCTACCTC 901 AAGCTCGGGC AGACCTCGAT GCAGGGCATC AAAGCCTATA TGCAGCAAAA 951 CCCGCAACGC CTCGCCGAAG TTTTGGGGCA AAACCCCAGC TATATCTTTT 1001 TCCGAGAGCT TACCGGAAGC AGCAATGACG GCCCTGTCGG CGCACTGGGC 1051 ACGCCGCTGA TGGGCGAGTA CGCCGGCGCA GTCGACCGGC ACTACATTAC 1101 CTTGGGCGCG CCCTTATTTG TCGCCACCGC CCATCCGGTT ACCCGCAAAG 1151 CCCTCAACCG CCTGATTATG GCGCAGGATA CCGGCAGCGC GATTAAAGGC 1201 GCGGTGCGCG TGGATTATTT TTGGGGATAC GGCGACGAAG CCGGCGAACT 1251 TGCCGGCAAA CAGAAAACCA CGGGATATGT CTGGCAGCTT CTGCCCAACG 1301 GTATGAAGCC CGAATACCGC CCGTAA This corresponds to the amino acid sequence <SEQ ID 2794; ORF 919.a>: a919.pep 1 MKKYLFRAAL CGIAAAILAA CQSKSIQTFP QPDTSVINGP DRPVGIPDPA 51 GTTVGGGGAV YTWPHLSLP HWAAQDFAKS LQSFRLGCAN LKNRQGWQDV 101 CAQAFQTPVH SVQAKQFFER YFTPWQVAGN GSLAGTVTGY YEPVLKGDDR 151 RTAQARFPIY GIPDDFISVP LPAGLRSGKA LVRIRQTGKN SGTIDNTGGT 201 HTADLSQFPI TARTTAIKGR FEGSRFLPYH TRNQINGGAL DGKAPILGYA 251 EDPVELFFMH IQGSGRLKTP SGKYIRIGYA DKNEHPYVSI GRYMADKGYL 301 KLGQTSMQGI KAYMQQNPQR LAEVLGQNPS YIFFRELTGS SNDGPVGALG 351 TPLMGEYAGA VDRHYITLGA PLFVATAHPV TRKALNRLIM AQDTGSAIKG 401 AVRVDYFWGY GDEAGELAGK QKTTGYVWQL LPNGMKPEYR P* m919/a919 98.6% identity in 441 aa overlap 10 20 30 40 50 60 m919.pep MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPDPAGTTVGGGGAV II I II II II I II II II I II II I II II I II II I II II II II I I II II I I II I I I II II II a919 MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPDRPVGIPDPAGTTVGGGGAV 10 20 30 40 50 60 70 80 90 100 110 120 919. pep YTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSFQAKQFFER II II II I II I II II II II I I II II I II II II II II II II I I II II II I I II I II I II II a919 YTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCAQAFQTPVHSVQAKQFFER 70 80 90 100 110 120 130 140 150 160 170 180 m919.pep YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA I II II II II I II I I II II I I II I II II II I II II II I I II II I II I II I II II II II I I I a919 YFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIYGIPDDFISVPLPAGLRSGKA 130 140 150 160 170 180 190 200 210 220 230 240 m919. pep LVRIRQTGKNSGTIDNTGGTHTADLSRFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL II II II I II II II I I II I II II II I I: I II II I II II II I I II II II I II I II I II II II a919 LVRIRQTGKNSGTIDNTGGTHTADLSQFPITARTTAIKGRFEGSRFLPYHTRNQINGGAL 190 200 210 220 230 240 250 260 270 280 290 300 m919.pep DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL II I II I I II I I I I II II I I II II I II II I I II II II I I II I II II I II I II II I II II II a919 DGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIRIGYADKNEHPYVSIGRYMADKGYL 250 260 270 280 290 300 310 320 330 340 350 360 m919.pep KLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFFRELAGSSNDGPVGALGTPLMGEYAGA II I II I I II I I: IM I II II I II II II II II II II I I : I I I II II I II II II II II II I I a919 KLGQTSMQGIKAYMQQNPQRLAEVLGQNPSYIFFRELTGSSNDGPVGALGTPLMGEYAGA 310 320 330 340 350 360 370 380 390 400 410 420 m919. pep VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK II II II I II II I I II II II II II I II II I II II II II I II I II I II I I I I I II I I I I II I a919 VDRHYITLGAPLFVATAHPVTRKALNRLIMAQDTGSAIKGAVRVDYFWGYGDEAGELAGK 370 380 390 400 410 420 430 440 m919. pep QKTTGYVWQLLPNGMKPEYRPX II I I II I I II I I I II II II II I a919 QKTTGYVWQLLPNGMKPEYRPX 430 440 Expression of ORF 919 The primer described in Example 1 for ORF 919 was used to locate and clone ORF 919. This sequence was purified and expressed in E. coli as provided in Figure 1 #. The hydrophilicity plots, antigenic index, and amphipatic regions of ORF 919 is provided in Figure 5 #. The AMPHI program is used to predict putative T-cell epitopes (Gao et al 1989, J Immunol 143:3007; Roberts et al. 996, AIDS Res Human Retroviruses 12:593; Quakyi et al. 1992, Scand J Immunol Suppl 11:9). The nucleic acid sequence of ORF 919 is provided in Exhibit C #. The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2795>: g920.seq (partial) 1 ..ccgatgcagc tggttaccga aaaAGGTAAG GAAAACATGA TTCAACGCGG 51 CACATACAAC TACCAATACC GCAGCAACCG TCCCGTCAAA GACGGCAGCT 101 ACCTCGTTAC CGCCGAATAT CAGCCTACTT TCCGGTCAAA AAACAAAGCA 151 GGCTGGAAAC AGGCTGGCAT CAAAGAAATG CCTGACGCAA GCTATTGCGA 201 ACAAACCCGT ATGTTCGGTA AAAACATTGT CAACGTGGGA CACGAAAGCG 251 CGGACACCGC CATCATCACC AAACCGGTCG GACAAAACTT GGAAATCGTC 301 CCGCTGGACA ATCccgccga caTTCACgtg ggctaacgCt tcaaaGTccg 351 cgttCtgttc cgtGGCgaac cgCTGcccaa tgccACCgtt accgCtacAT 401 TTGacggctt cGAcaccagc gaccgcagca aaacgcacaa Aaccgaagcc 451 caagcctTCT ccgacaccac cgacggcgaa ggcgaagtgg acatcatCCC 501 CTTGCgccaa GGCTTttgga aAgcGAGTGT CGAATAcaaa gccgAtttcc 551 CCGATcaaAG CCTGTGccga AAACAggcgA ACTACaCaac TTtaaccttc 601 caaatcgccc attctCacca tTAa This corresponds to the amino acid sequence <SEQ ID 2796; ORF 920.ng>: g920.pep (partial) 1 .. PMQLVTEKGK EΝMIQRGTYΝ YQYRSΝRPVK DGSYLVTAEY QPTFRSKΝKA 51 GWKQAGIKEM PDASYCEQTR MFGKΝIVΝVG HESADTAIIT KPVGQΝLEIV 101 PLDΝPADIHV GXRFKVRVLF RGEPLPΝATV TATFDGFDTS DRSKTHKTEA 151 QAFSDTTDGE GEVDIIPLRQ GFWKASVEYK ADFPDQSLCR KQAΝYTTLTF 201 QIAHSHH* The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 2797>: m920.seq 1 ATGAAGAAAA CATTGACACT GCTCTCCGTT TCCGCCCTAT TTGCCACATC 51 CGCCCACGCC CACCGmGTCT GGGTCGAAAC CGCCCACACG CACGGCGGCG 101 AATACCTTAA AGCCGACTTG GGCTACGGCG AATTTCCCGA ACTCGAACCC 151 ATCGCCAAAG ACCGCCTGCA CATCTTCAGC AAACCGATGC AGCTGGTTAC 201 CGAAAAAGGC AAGGAAAACA TGATTCAACG CGGCACATAC AACTACCAGT 251 ACCGAAGCAA CCGTCCCGTT AAGGACGGCA GTTACCTCGT CATCGCCGAA 301 TATCAGCCTA CTTTCTGGTC AAAAwACAAA GCAGGCTGGA AACAGGCGGG 351 CATCAAAGAA ATGCCTGACG CAAGCTATTG CGAACAAACC CGAATGTTCG 401 GCAAAAACAT CGTCAACGTC GGACACGAAA GCGCGGACAC CGCCATCATC 451 ACCAArCCGG TCGGACAAAA CTTGGAAATC GTCCCGCTGG ACAATCCCGC 501 CAACATTCAC GTAGGCGAAC GCTTCAAAGT CCGCGTTCTG TTCCGTGGCG 551 AACCGCTGCC CAATGCCACC GTTACCGCCA CCTTTGACGG CTTCGACACC 601 AGCGACCGCA GCAAAACGCA CAAwmCCGAA GCACAGGCTT TCTCCGACAG 651 CACAGACGAC AAAGGCGAAG TGGACATCAT CmCCTTGCGC CAAGGCTTCT 701 GGAAAGCCAA TGTCGAACAC AAAACCGACT TCCCCGATCA AAGCGTGTGC 751 CAAAAACAGG CGAACTACTC GACTTTAACC TTCCAAATCG GTCATTCGCA 801 CCATTAA This corresponds to the amino acid sequence <SEQ ID 2798; ORF 920>: m920.pep 1 MKKTLTLLSV SALFATSAHA HRVWVETAHT HGGEYLKADL GYGEFPELEP 51 IAKDRLHIFS KPMQLVTEKG KEΝMIQRGTY ΝYQYRSΝRPV KDGSYLVIAE 101 YQPTFWSKXK AGWKQAGIKE MPDASYCEQT RMFGKΝIVΝV GHESADTAII 151 TKPVGQΝLEI VPLDΝPAΝIH VGERFKVRVL FRGEPLPΝAT VTATFDGFDT 201 SDRSKTHXXE AQAFSDSTDD KGEVDIIXLR QGFWKANVEH KTDFPDQSVC 251 QKQANYSTLT FQIGHSHH* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 920 shows 91.3% identity over a 207 aa overlap with a predicted ORF (ORF 920.ng) from N gonorrhoeae: g920/m920 10 20 30 g920.pep PMQLVTEKGKEΝMIQRGTYΝYQYRSΝRPVK m920 GGEYLKADLGYGEFPELEPIAKDRLHIFSKPMQLVTEKGKEΝMIQRGTYΝYQYRSΝRPVK 40 50 60 70 80 90 40 50 60 70 80 90 g920.pep DGSYLVTAEYQPTFRSKΝKAGWKQAGIKEMPDASYCEQTRMFGKΝIVΝVGHESADTAIIT M M M M I I I M I I I I 1 1 I I I I I I I I I I I I I I I I I I I I I 1 1 1 I I I I I I I I I I I I I I m920 DGSYLVIAEYQPTFWSKXKAGWKQAGIKEMPDASYCEQTRMFGKΝIVΝVGHESADTAIIT 100 110 120 130 140 150 100 110 120 130 140 150 g920. pep KPVGQΝLEIVPLDΝPADIHVGXRFKVRVLFRGEPLPΝATVTATFDGFDTSDRSKTHKTEA Ml MMMMMMMMIMMMMMIMMM =11 m920 KPVGQΝLEIVPLDΝPAΝIHVGERFKVRVLFRGEPLPΝATVTATFDGFDTSDRSKTHXXEA 160 170 180 190 200 210 160 170 180 190 200 g920.pep QAFSDTTDGEGEVDIIPLRQGFWKASVEYKADFPDQSLCRKQAΝYTTLTFQIAHSHHX H l l h l l = 1 1 1 1 1 1 M I M M h l h h l M M h h M M h M M I h l M M m920 QAFSDSTDDKGEVDIIXLRQGFWKAΝVEHKTDFPDQSVCQKQAΝYSTLTFQIGHSHHX 220 230 240 250 260 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2799>: a920.seq 1 TGAAAGAAAA CATTGACACT GCTCGCCGTT TCCGCCCTAT TTGCCGCATC 51 CGCCCACGCC CACCGCGTCT GGGTCGAAAC CGCCCACACG CACGGCGGCG 101 AATACCTTAA AGCCGACTTG GGCTACGGCG AATTTCCCGA ACTCGAACCC 151 ATCGCCAAAG ACCGCCTGCA CATCTTCAGC AAACCGATGC AGCTGGTTAC 201 CGAAAAAGGC AAGGAAAACA TGATTCAACG CGGCACATAC AACTACCAGT 251 ACCGAAGCAA CCGTCCCGTT AAGGACGGCA GTTACCTCGT CATCGCCGAA 301 TATCAGCCTA CTTTCTGGTC AAAAAACAAA GCAGGCTGGA AACAGGCGGG 351 CATCAAACAA ATGCCTGACG CAAGCTATTG CGAACAAACC CGAATGTTCG 401 GCAAAAACAT CGTCAACGTC GGACACGAAA GCGCGGACAC CGCCATCATC 451 ACCAAACCGG TCGGACAAAA CTTGGAAATC GTCCCGCTGG ACAATCCCGC 501 CAACATTCAC GTAGGCGAAC GCTTCAAAGT CCGCGTTCTG TTCCGTGGCG 551 AACCGCTGCC CAATGCCACC GTTACCGCCA CCTTTGACGG CTTCGACACC 601 AGCGACCGCA GCAAAACGCA CAAAACCGAA GCACAGGCTT TCTCCGACAG 651 CACAGACGAC AAAGGCGAAG TGGACATCAT CCCCTTGCGC CAAGGCTTCT 701 GGAAAGCCAA TGTCGAACAC AAAGCCGACT TCCCCGATCA AAGCGTGTGC 751 CAAAAACAGG CGAACTACTC GACTTTAACC TTCCAAATCG GCCATTCGCA 801 CCATTAA This corresponds to the amino acid sequence <SEQ ID 2800; ORF 920. a>: a920.pep 1 *KKTLTLLAV SALFAASAHA HRVWVETAHT HGGEYLKADL GYGEFPELEP 51 IAKDRLHIFS KPMQLVTEKG KEΝMIQRGTY ΝYQYRSΝRPV KDGSYLVIAE 101 YQPTFWSKΝK AGWKQAGIKQ MPDASYCEQT RMFGKΝIVΝV GHESADTAII 151 TKPVGQΝLEI VPLDΝPAΝIH VGERFKVRVL FRGEPLPΝAT VTATFDGFDT 201 SDRSKTHKTE AQAFSDSTDD KGEVDIIPLR QGFWKANVEH KADFPDQSVC 251 QKQANYSTLT FQIGHSHH* m920/a920 97.0% identity in 267 aa overlap 10 20 30 40 50 60 m920.pep MKKTLTLLSVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGEFPELEPIAKDRLHIFS II II II I: II I II I : II II II II II I II II II I I II I II II I II II I II I I II II II II a920 XKKTLTLLAVSALFAASAHAHRVWVETAHTHGGEYLKADLGYGEFPELEPIAKDRLHIFS 10 20 30 40 50 60 70 80 90 100 110 120 m920. pep KPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQPTFWSKXKAGWKQAGIKE II I II II II II II II I II I II II I II II II II II II I I I II II II I II a920 KPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQPTFWSKNKAGWKQAGIKQ 70 80 90 100 110 120 130 140 150 160 170 180 m920.pep MPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEIVPLDNPANIHVGERFKVRVL II II II I II II II I II II II II I II II I II I I II II I I I II II II II II II I I II II I II a920 MPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEIVPLDNPANIHVGERFKVRVL 130 140 150 160 170 180 190 200 210 220 230 240 m920.pep FRGEPLPNATVTATFDGFDTSDRSKTHXXEAQAFSDSTDDKGEVDIIXLRQGFWKANVEH II II II II II II I I II II II I II I II I : II II I I II II II I I II II I I I I I I I II II I a920 FRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDSTDDKGEVDIIPLRQGFWKANVEH 190 200 210 220 230 240 250 260 269 m920 . pep KTDFPDQSVCQKQANYSTLTFQIGHSHHX M M M M M M M M M M M I M M M a920 KADFPDQSVCQKQANYSTLTFQIGHSHHX 250 260 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2801>: g920-l.seq 1 ATGAAGAAAA CATTGACACT GCTCGCcgtt TcCGCACTAT TTGCCACATc 51 cgCaCACCCC CACCgCGTCT GGGTCGAAAC CgccCACACg cAcgGCGGCG 101 AATACCTTAA AGCCGACTTG GGCTACGGCG AATTCCCCGA ACTCGAACCC 151 ATCGccAAAG ACCgccTGCA CATCTTCAGC AAACCGATGC AGCTGGTTAC 201 CGAAAAAGGT AAGGAAAACA TGATTCAACG CGGCACATAC AACTACCAAT 251 ACCGCAGCAA CCGTCCCGTC AAAGACGGCA GCTACCTCGT TACCGCCGAA 301 TATCAGCCTA CTTTCCGGTC AAAAAACAAA GCAGGCTGGA AACAGGCTGG 351 CATCAAAGAA ATGCCTGACG CAAGCTATTG CGAACAAACC CGTATGTTCG 401 GTAAAAACAT TGTCAACGTG GGACACGAAA GCGCGGACAC CGCCATCATC 451 ACCAAACCGG TCGGACAAAA CTTGGAAATC GTCCCGCTGG ACAATCCCGC 501 CAACATTCAC GTAGGCGAAC GCTTCAAAGT CCGCGTTCTG TTCCGTGGCG 551 AACCGCTGCC CAATGCCACC GTTACCGCTA CATTTGACGG CTTCGACACC 601 AGCGACCGCA GCAAAACGCA CAAAACCGAA GCCCAAGCCT TCTCCGACAC 651 CACCGACGGC AAAGGCGAAG TGGACATCAT CCCCTTGCGC CAAGGCTTTT 701 GGAAAGCGAG TGTCGAATAC AAAGCCGATT TCCCCGATCA AAGCCTGTGC 751 CAAAAACAGG CGAACTACAC AACTTTAACC TTCCAAATCG GCCATTCTCA 801 CCATTAA This corresponds to the amino acid sequence <SEQ ID 2802; ORF 920-l.ng>: 1 MKKTLTLLAV SALFATSAHP HRVWVETAHT HGGEYLKADL GYGEFPELEP 51 IAKDRLHIFS KPMQLVTEKG KEΝMIQRGTY ΝYQYRSΝRPV KDGSYLVTAE 101 YQPTFRSKΝK AGWKQAGIKE MPDASYCEQT RMFGKΝIVΝV GHESADTAII 151 TKPVGQΝLEI VPLDΝPAΝIH VGERFKVRVL FRGEPLPΝAT VTATFDGFDT 201 SDRSKTHKTE AQAFSDTTDG KGEVDIIPLR QGFWKASVEY KADFPDQSLC 251 QKQAΝYTTLT FQIGHSHH* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2803>: m920-l.seq 1 ATGAAGAAAA CATTGACACT GCTCGCCGTT TCCGCCCTAT TTGCCACATC 51 CGCCCACGCC CACCGCGTCT GGGTCGAAAC CGCCCACACG CACGGCGGCG 101 AATACCTTAA AGCCGACTTG GGCTACGGCG AATTTCCCGA ACTCGAACCC 151 ATCGCCAAAG ACCGCCTGCA CATCTTCAGC AAACCGATGC AGCTGGTTAC 201 CGAAAAAGGC AAGGAAAACA TGATTCAACG CGGCACATAC AACTACCAGT 251 ACCGAAGCAA CCGTCCCGTT AAGGACGGCA GTTACCTCGT CATCGCCGAA 301 TATCAGCCTA CTTTCTGGTC AAAAAACAAA GCAGGCTGGA AACAGGCGGG 351 CATCAAAGAA ATGCCTGACG CAAGCTATTG CGAACAAACC CGAATGTTCG 401 GCAAAAACAT CGTCAACGTC GGACACGAAA GCGCGGACAC CGCCATCATC 451 ACCAAACCGG TCGGACAAAA CTTGGAAATC GTCCCGCTGG ACAATCCCGC 501 CAACATTCAC GTAGGCGAAC GCTTCAAAGT CCGCGTTCTG TTCCGTGGCG 551 AACCGCTGCC CAATGCCACC GTTACCGCCA CCTTTGACGG CTTCGACACC 601 AGCGACCGCA GCAAAACGCA CAAAACCGAA GCACAGGCTT TCTCCGACAG 651 CACAGACGAC AAAGGCGAAG TGGACATCAT CCCCTTGCGC CAAGGCTTCT 701 GGAAAGCCAA TGTCGAACAC AAAACCGACT TCCCCGATCA AAGCGTGTGC 751 CAAAAACAGG CGAACTACTC GACTTTAACC TTCCAAATCG GTCATTCGCA 801 CCATTAA This corresponds to the amino acid sequence <SEQ ID 2804; ORF 920- 1>: m920-l.ρep 1 KKTLTLLAV SALFATΞAHA HRVWVETAHT HGGEYLKADL GYGEFPELEP 51 IAKDRLHIFS KPMQLVTEKG KENMIQRGTY NYQYRSNRPV KDGSYLVIAE 101 YQPTFWSKNK AGWKQAGIKE MPDASYCEQT RMFGKNIVNV GHESADTAII 151 TKPVGQNLEI VPLDNPANIH VGERFKVRVL FRGEPLPNAT VTATFDGFDT 201 SDRΞKTHKTE AQAFSDSTDD KGEVDIIPLR QGFWKANVEH KTDFPDQSVC 251 QKQANYSTLT FQIGHSHH* 920-l/g920-l 96.3% identity in 268 aa overlap 10 20 30 40 50 60 m920-l.pep KKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGEFPELEPIAKDRLHIFS I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g920-l MKKTLTLLAVΞALFATΞAHPHRVWVETAHTHGGEYLKADLGYGEFPELEPIAKDRLHIFS 10 20 30 40 50 60 70 80 90 100 110 120 m920-l.pep KPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQPTFWΞKNKAGWKQAGIKE I I II I I I I I I I I I I II I I I I I I I I I I I I I I II I I I I I llll g920-l KPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVTAEYQPTFRSKNKAGWKQAGIKE 70 80 90 100 110 120 130 140 150 160 170 180 m920-l.pep MPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEIVPLDNPANIHVGERFKVRVL I I II I I I I I I I I I I I I I I I I I I I llllll I I I I I I I I I I I I I I I I I I I I II II I II I I I I g920-l MPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEIVPLDNPANIHVGERFKVRVL 130 140 150 160 170 180 190 200 210 220 230 240 m920-l.pep FRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDSTDDKGEVDIIPLRQGFWKANVEH II II I I I I I I II I III I M I I I I I I II I I I I II Ml I I I I I I I I I I I I I I I I Ml M g920-l FRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDTTDGKGEVDIIPLRQGFWKASVEY 190 200 210 220 230 240 250 260 269 m920-l.pep KTDFPDQSVCQKQANYSTLTFQIGHSHHX g920-l KADFPDQSLCQKQANYTTLTFQIGHSHHX 250 260 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2805>: a920.seq 1 TGAAAGAAAA CATTGACACT GCTCGCCGTT TCCGCCCTAT TTGCCGCATC 51 CGCCCACGCC CACCGCGTCT GGGTCGAAAC CGCCCACACG CACGGCGGCG 101 AATACCTTAA AGCCGACTTG GGCTACGGCG AATTTCCCGA ACTCGAACCC 151 ATCGCCAAAG ACCGCCTGCA CATCTTCAGC AAACCGATGC AGCTGGTTAC 201 CGAAAAAGGC AAGGAAAACA TGATTCAACG CGGCACATAC AACTACCAGT 251 ACCGAAGCAA CCGTCCCGTT AAGGACGGCA GTTACCTCGT CATCGCCGAA 301 TATCAGCCTA CTTTCTGGTC AAAAAACAAA GCAGGCTGGA AACAGGCGGG 351 CATCAAACAA ATGCCTGACG CAAGCTATTG CGAACAAACC CGAATGTTCG 401 GCAAAAACAT CGTCAACGTC GGACACGAAA GCGCGGACAC CGCCATCATC 451 ACCAAACCGG TCGGACAAAA CTTGGAAATC GTCCCGCTGG ACAATCCCGC 501 CAACATTCAC GTAGGCGAAC GCTTCAAAGT CCGCGTTCTG TTCCGTGGCG 551 AACCGCTGCC CAATGCCACC GTTACCGCCA CCTTTGACGG CTTCGACACC 601 AGCGACCGCA GCAAAACGCA CAAAACCGAA GCACAGGCTT TCTCCGACAG 651 CACAGACGAC AAAGGCGAAG TGGACATCAT CCCCTTGCGC CAAGGCTTCT 701 GGAAAGCCAA TGTCGAACAC AAAGCCGACT TCCCCGATCA AAGCGTGTGC 751 CAAAAACAGG CGAACTACTC GACTTTAACC TTCCAAATCG GCCATTCGCA 801 CCATTAA This corresponds to the amino acid sequence <SEQ ID 2806; ORF 920-1. a>: a920.pep 1 *KKTLTLLAV SALFAASAHA HRVWVETAHT HGGEYLKADL GYGEFPELEP 51 IAKDRLHIFS KPMQLVTEKG KENMIQRGTY NYQYRSNRPV KDGSYLVIAE 101 YQPTFWSKNK AGWKQAGIKQ MPDASYCEQT RMFGKNIVNV GHESADTAII 151 TKPVGQNLEI VPLDNPANIH VGERFKVRVL FRGEPLPNAT VTATFDGFDT 201 SDRSKTHKTE AQAFSDSTDD KGEVDIIPLR QGFWKANVEH KADFPDQSVC 251 QKQANYSTLT FQIGHSHH* m920-l/a920 98.9% identity in 267 aa overlap 10 20 30 40 50 60 m920-l.pep MKKTLTLLAVΞALFATSAHAHRVWVETAHTHGGEYLKADLGYGEFPELEPIAKDRLHIFS I I I I I I I I I I I I I l I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a920 XKKTLTLLAVΞALFAASAHAHRVWVETAHTHGGEYLKADLGYGEFPELEPIAKDRLHIFS 10 20 30 40 50 60 70 80 90 100 110 120 m920-l.pep KPMQLVTEKGKEN IQRGTYNYQYRSNRPVKDGSYLVIAEYQPTFWSKNKAGWKQAGIKE I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I : a920 KPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQPTFWSKNKAGWKQAGIKQ 70 80 90 100 110 120 130 140 150 160 170 180 m920-l.pep PDASYCEQTRMFGKNIVNVGHEΞADTAIITKPVGQNLEIVPLDNPANIHVGERFKVRVL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a920 MPDASYCEQTRMFGKNIVNVGHEΞADTAIITKPVGQNLEIVPLDNPANIHVGERFKVRVL 130 140 150 160 170 180 190 200 210 220 230 240 m920-l.pep FRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDSTDDKGEVDIIPLRQGFWKANVEH I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a920 FRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDSTDDKGEVDIIPLRQGFWKANVEH 190 200 210 220 230 240 250 260 269 m920-l .pep KTDFPDQSVCQKQANYSTLTFQIGHSHHX I : I I I I I I I I I I I I I I I I I I I I I I I I I I I a920 KADFPDQSVCQKQANYΞTLTFQIGHSHHX 250 260 The following partial DNA sequence was identified in N.gonorrhoeae <SEQ ID 2807>: g921.seq 1 ATGAAAAAAT ACCTTATCCC TCTTTCCATT GCGGCAGTCC TTTCCGggtG 51 Ccagtctatt tatGtgccca cattgacggA aatccccgTg aatcccatca 101 ataCCgtcaa aacggaagCA CCTGCAAAAG GTTTTCGCCT CGCCCCTTCG 151 CATTGGGCGG ATGTTGCCAA AATCAGCGAT GAAGCGACGC GCTTGGGCTA 201 TCAGGTGGGT ATCGGTAAAA TGACCAAGGT TCAGGcgGCG CAATATCTGA 251 ACAACTTCAG AAAACGCCTG GTCGGACGCA ATGCCGTCGA TGACAGTATG 301 TATGAAATCT ACCTGCGTTC GGCGGTAGAC AGCCAGCGCG GCGAAATCAA 351 TACGGAACAG TCCAAGCTGT ATATCGAGAA TGCCTTGCGC GGCTGGCAGC 401 AGCGTtggAA AAATATGGAT GCCAAACCCG ATAATCCCGC ATTTACCAAC 451 TTTTTGATGG AAGTGATGAA GATGCAGCCC TTGAAATGA This corresponds to the amino acid sequence <SEQ ID 2808; ORF 921.ng>: g921.pep 1 MKKYLIPLSI AAVLSGCQSI YVPTLTEIPV NPINTVKTEA PAKGFRLAPS 51 HWADVAKISD EATRLGYQVG IGKMTKVQAA QYLNNFRKRL VGRNAVDDSM 101 YEIYLRSAVD SQRGEINTEQ SKLYIENALR GWQQRWKNMD AKPDNPAFTN 151 FLMEVMKMQP LK* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2809>: m921.seq 1 ATGAAAAAAT ACCTTATCCC TCTTTCCATT GCGGCAGTTC TTTCCGGCTG 51 CCAGTCTATT TATGTGCCCA CATTGACGGA AATCCCCGTG AATCCTATCA 101 ATACCGTCAA AACGGAAGCA CCTGCAAAAG GTTTCCGCCT TGCCTCTTCG 151 CATTGGACGG ATGTTGCCAA AATCAGCGAT GAAGCGACGC GCTTGGGCTA 201 TCAGGTGGGT ATCGGTAAAA TGACCAAGGT TCAGGCGGCG CAATATCTGA 251 ACAACTTCAG AAAACGCCTG GTCGGACGCA ATGCCGTCGA TGACAGTATG 301 TATGAAATCT ACCTGCGTTC GGCGATAGAC AGCCAGCGGG GCGCAATCAA 351 TACGGAACAG TCCAAGCTGT ATATCCAGAA TGCCTTGCGC GGCTGGCAGC 401 AGCGTTGGAA AAATATGGAT GTCAAACCCA ACAACCCCGC ATTTACCAAC 451 TTTTTGATGG AAGTGATGAA GATGCAGCCC TTGAAATGA This corresponds to the amino acid sequence <SEQ ID 2810; ORF 921>: m921.pep 1 MKKYLIPLSI AAVLSGCQSI YVPTLTEIPV NPINTVKTEA PAKGFRLASS 51 HWTDVAKISD EATRLGYQVG IGKMTKVQAA QYLNNFRKRL VGRNAVDDSM 101 YEIYLRSAID SQRGAINTEQ SKLYIQNALR GWQQRWKNMD VKPNNPAFTN 151 FLMEVMKMQP LK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 921 shows 95.7% identity over a 162 aa overlap with a predicted ORF (ORF 921.ng) from N gonorrhoeae: m92l/g921 10 20 30 40 50 60 m921.pep MKKYLIPLSIAAVLSGCQSIYVPTLTEIPVΝPIΝTVKTEAPAKGFRLASSHWTDVAKISD MMMMMMMMMIMMMMMIMMMMIIMMMM MhlllMM g921 MKKYLIPLSIAAVLSGCQSIYVPTLTEIPVΝPIΝTVKTEAPAKGFRLAPSHWADVAKISD 10 20 30 40 50 60 70 80 90 100 110 120 m921. pep EATRLGYQVGIGKMTKVQAAQYLΝΝFRKRLVGRΝAVDDSMYEIYLRSAIDSQRGAIΝTEQ IMIMMMMMMMMMMMMMIMMIMMMMMIhlllll Mill g921 EATRLGYQVGIGKMTKVQAAQYLΝΝFRKRLVGRΝAVDDSMYEIYLRSAVDSQRGEIΝTEQ 70 80 90 100 110 120 130 140 150 160 m921. pep SKLYIQΝALRGWQQRWKΝMDVKPΝΝPAFTΝFLMEVMKMQPLKX llllhlMMMMMMhlhlMIIMMMMMMM g921 SKLYIEΝALRGWQQRWKΝMDAKPDΝPAFTΝFLMEVMKMQPLKX 130 140 150 160 The following partial DΝA sequence was identified inN meningitidis <SEQ ID 2811>: a921.seq 1 ATGAAAAAAT ACCTTATCCC TCTTTCCATT GTGGCAGTTC TTTCCGGCTG 51 CCAGTCTATT TATGTGCCCA CATTGACGGA AATCCCCGTG AATCCTATCA 101 ATACCGTCAA AACGGAAGCA CCTGCAAAAG GTTTCCGCCT TGCCTCTTCG 151 CATTGGACGG ATGTTGCCAA AATCAGCGAT GAAGCGACGC GCTTGGGCTA 201 TCAGGTGGGT ATCGGTAAAA TGACCAAGGT TCAGGCGGCG CAATATCTGA 251 ACAACTTCAG AAAACGCCTG GTCGGACGCA ATGCCGTCGA TGACAGTATG 301 TATGAAATCT ACCTGCGTTC GGCGATAGAC AGCCAGCGGG GCGCAATCAA 351 TACGGAACAG TCCAAGCTGT ATATCCAGAA TGCCTTGCGC GGCTGGCAGC 401 AGCGTTGGAA AAATATGGAT GTCAAACCCA ACAACCCCGC ATTTACCAAC 451 TTTTTGATGG AAGTGATGAA GATGCAGCCC TTGAAATGA This corresponds to the amino acid sequence <SEQ ID 2812; ORF 921. a>: a921.pep 1 MKKYLIPLSI VAVLSGCQSI YVPTLTEIPV ΝPIΝTVKTEA PAKGFRLASS 51 HWTDVAKISD EATRLGYQVG IGKMTKVQAA QYLΝΝFRKRL VGRNAVDDSM 101 YEIYLRSAID SQRGAINTEQ SKLYIQNALR GWQQRWKNMD VKPNNPAFTN 151 FLMEVMKMQP LK* m921/a921 99.4% identity in 162 aa overlap 10 20 30 40 50 60 m921.pep MKKYLIPLSIAAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKGFRLASSHWTDVAKISD I M II II I II M II II I II I II I II II II I II I II II I I I I II I II II I I II II II I I I I a921 MKKYLIPLSIVAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKGFRLASSHWTDVAKISD 10 20 30 40 50 60 70 80 90 100 110 120 m921.pep EATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYEIYLRSAIDSQRGAINTEQ MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a921 EATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYEIYLRSAIDSQRGAINTEQ 70 80 90 100 110 120 130 140 150 160 m921. pep SKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQPLKX II II I I II I I I II I II I II II I I II II II I II II I II II II II a921 SKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQPLKX 130 140 150 160 The following partial DNA sequence was identified in N.gonorrhoeae <SEQ LD 2813>: g922.seq 1 ATGGAAAAGA GAAAAATACT GCCGCTGGCA ATTTGTTTGG CGGCTTTGTC 51 TGCCTGTACG GCGATGGAGG CCCGCACACC CCGGGCAAAT GAAGCCCAAG 101 CCCCCCGCGC GGATGAAATG AAAAAAGAAA GCCGCCCCGC GTTTGACGCG 151 GCAGCCGTAC CGGTATCCGA CAGCGGGTTT GCCGCCAATG CAAATGTCCG 201 CCGTTTTGTG GACGATGAAG TCGGGAAAGG GGATTTTTCC CAGGCGGAAT 251 GGCAGGATTT TTTTGACAAA GCGGCTTACA AGGCGGACAT CGTCAAGATt 301 ATGCACCGAC CCTCCACATC GCGtCCGTGG TATGtgttcc gCacggGAAa 351 ttcGGgcagg gcgaaAtttc ACggcgCGCG Caggttttat GcggaaAacc 401 gcgcggttat cgatgatgtg gcgCAAAAat acggcgtGCC TGCCGAGCTT 451 ATCGTGGCGA TTATCGGGAT TGAAACGAAT TACGGCAAAA ATACGGGCAG 501 TTTCCGTGTG GCGGACGCAT TGGCGACTTT AGGCTTTGAT TATCCCCGCC 551 GCGCCGGGTT TTTCCAAAAA GAATTGGTCG AGCTTTTAAA GCTGGCAAAA 601 GAAGAAGGCG GTGATGTTTT CGCCTTTAAG GGCagcTATG CGGGTGCAAT 651 GGGTATGCCG CAATTTATGC CTTCGAGCTA CCGGAAATGG GCGGTGGATT 701 ATGAcgggga cggacatCGG GATATAtggg GCAACGTcgg tgatgtcgcg 751 gcatcggTTG CCAATTAtat gaagCAGCAC GGTTGGCGCA CgggcggtAA 801 AATGTTGGTG TCGGCGAcgt tggcgccggg tgcggATGTT CAggcAATCA 851 TTGGCGAAAA AACCGCCCTG ACGCGGACGG TGGCGGATTT GAaggCGTAc 901 ggcatcatcc ccggggaaaC GCTCGCAGAT GATGAAAAGg cgGTTTTGTT 951 CAAACTGGAA ACCGCACCCG GCGTGTTTGA ATATTATTTG GGCTTGAACA 1001 ATTTTTATAC GGTATGGCAG TACAACCACA GCCGGATGTA TGTAACGgcg 1051 gtcaggGACA TTGCCAATTC GCTCGGCGGC CCGGGATTGT Aa This corresponds to the amino acid sequence <SEQ ID 2814; ORF 922.ng>: g922.pep 1 MEKRKILPLA ICLAALSACT AMEARTPRAN EAQAPRADEM KKESRPAFDA 51 AAVPVSDSGF AANANVRRFV DDEVGKGDFS QAEWQDFFDK AAYKADIVKI 101 MHRPSTSRPW YVFRTGNSGR AKFHGARRFY AENRAVIDDV AQKYGVPAEL 151 IVAIIGIETN YGKNTGSFRV ADALATLGFD YPRRAGFFQK ELVELLKLAK 201 EEGGDVFAFK GSYAGAMGMP QFMPSSYRKW AVDYDGDGHR DIWGNVGDVA 251 ASVANYMKQH GWRTGGKMLV SATLAPGADV QAIIGEKTAL TRTVADLKAY 301 GIIPGETLAD DEKAVLFKLE TAPGVFEYYL GLNNFYTVWQ YNHSRMYVTA 351 VRDIANSLGG PGL* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2815>: m922.seq 1 ATGAAAAAGA GAAAAATACT GCCGCTGGCA ATTTGTTTGG CGGCTTTGTC 51 TGCCTGTACG GCGATGGAGG CACGCCCACC CCGGGCAAAT GAAGCCCAAG 101 CCCCCCGCGC GGTTGAAATG AAAAAAGAAA GCCGCCCCGC GTTTGACGCG 151 GCAGCCGTAT TTGACGCGGC AGCCGTACCG GTATCCGACA GCGGGTTTGC 201 CGCCAATGCA AATGTCCGCC GTTTTGTGGA CGATGAAGTC GGGAAAGGGG 251 ATTTTTCCCG GGCGGAATGG CAGGATTTTT TTGACAAAGC GGCTTACAAG 301 GCGGACATCG TCAAGATTAT GCACCGCCCC TCCACATCGC GTCCGTGGTA 351 TGTGTTCCGC ACGGGAAATT CGGGCAAGGC GAAATTTCGC GGCGCGCGCC 401 GGTTTTATGC GGAAAACCGC GCGCTTATCG ATGATGTGGC GCAAAAATAC 451 GGCGTGCCTG CCGAACTTAT CGTGGCGGTT ATCGGGATTG AAACGAATTA 501 CGGCAAAAAT ACGGGCAGTT TCCGTGTGGC GGACGCATTG GCGACCTTAG 551 GCTTTGATTA CCCCCGCCGC GCCGGGTTTT TCCAAAAAGA ATTGGTCGAG 601 CTTTTAAAGC TGGCAAAAGA AGAAGGCGGC GATGTTTTCG CCTTTAAAGG 651 CAGCTATGCG GGCGCAATGG GGATGCCGCA ATTTATGCCT TCGAGCTACC 701 GGAAATGGGC GGTGGATTAT GACGGGGACG GACATCGGGA CATATGGGGC 751 AACGTCGGCG ATGTCGCGGC ATCGGTTGCC AATTATATGA AGCAGCACGG 801 TTGGCGCACG GGCGGGAAAA TGCTGGTGTC TGCAACATTG GCGCCGGGTG 851 CGGATGTTCA GGCAATCATT GGCGAAAAAA CCGCCCTGAC GCGGACGGTG 901 GCGGATTTGA AGGCGTACGG CATCATCCCC GGCGAAGAGC TTGCAGATGA 951 TGAAAAGGCG GTTTTGTTCA AACTGGAAAC CGCACCGGGC GTGTTTGAAT 1001 ATTATTTGGG CTTGAACAAT TTTTATACGG TATGGCAGTA CAACCACAGC 1051 CGGATGTATG TAACGGCGGT CAGGGACATT GCCAATTCGC TTGGCGGCCC 1101 GGGATTGTAA This corresponds to the amino acid sequence <SEQ ID 2816; ORF 922>: m922.pep 1 MKKRKILPLA ICLAALSACT AMEARPPRAN EAQAPRAVEM KKESRPAFDA 51 AAVFDAAAVP VSDSGFAANA NVRRFVDDEV GKGDFSRAEW QDFFDKAAYK 101 ADIVKIMHRP STSRPWYVFR TGNSGKAKFR GARRFYAENR ALIDDVAQKY 151 GVPAELIVAV IGIETNYGKN TGSFRVADAL ATLGFDYPRR AGFFQKELVE 201 LLKLAKEEGG DVFAFKGSYA GAMGMPQFMP SSYRKWAVDY DGDGHRDIWG 251 NVGDVAASVA NYMKQHGWRT GGKMLVSATL APGADVQAII GEKTALTRTV 301 ADLKAYGIIP GEELADDEKA VLFKLETAPG VFEYYLGLNN FYTVWQYNHS 351 RMYVTAVRDI ANSLGGPGL* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 922 shows 95.9% identity over a 369 aa overlap with a predicted ORF (ORF 922.ng) from N gonorrhoeae: m922/g922 10 20 30 40 50 60 m922.pep MKKRKILPLAICLAALSACTAMEARPPRAΝEAQAPRAVEMKKESRPAFDAAAVFDAAAVP I I I g922 MEKRKILPLAICLAALSACTAMEARTPRAΝEAQAPRADEMKKESRPAFDAA AVP 10 20 30 40 50 70 80 90 100 110 120 m92 .pep VSDSGFAAΝAΝVRRFVDDEVGKGDFSRAEWQDFFDKAAYKADIVKIMHRPSTSRPWYVFR MMMIMMMMIMMMMIhMMMIMMMMMMMMMMMMM g9 2 VSDSGFAAΝAΝVRRFVDDEVGKGDFSQAEWQDFFDKAAYKADIVKIMHRPSTSRPWYVFR 60 70 80 90 100 110 130 140 150 160 170 180 m92 .pep TGΝSGKAKFRGARRFYAEΝRALIDDVAQKYGVPAELIVAVIGIETΝYGKΝTGSFRVADAL I II II H I hill I Ml 111 hi II 111111 M 11111 11111 II I II I II 1111111 g922 TGΝSGRAKFHGARRFYAEΝRAVIDDVAQKYGVPAELIVAIIGIETΝYGKΝTGSFRVADAL 120 130 140 150 160 170 190 200 210 220 230 240 m92 .pep ATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGSYAGAMGMPQFMPSSYRKWAVDY MMMIMMMMMMMMMMMMMMMMMMMMMMIMMMM g922 ATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGSYAGAMGMPQFMPSSYRKWAVDY 180 190 200 210 220 230 250 260 270 280 290 300 m92 .pep DGDGHRDIWGΝVGDVAASVAΝYMKQHGWRTGGKMLVSATLAPGADVQAIIGEKTALTRTV M i l l I I I M i l l 1 1 M M M M M M M M M I I M i l l I I I I I I g922 DGDGHRDIWGΝVGDVAASVAΝYMKQHGWRTGGKMLVSATLAPGADVQAI IGEKTALTRTV 240 250 260 270 280 290 310 320 330 340 350 360 m922.pep ADLKAYGIIPGEELADDEKAVLFKLETAPGVFEYYLGLΝΝFYTVWQYΝHSRMYVTAVRDI M I M M M I M M M M M M M M M M M M M M I M M M M g922 ADLKAYGIIPGETLADDEKAVLFKLETAPGVFEYYLGLΝΝFYTVWQYΝHSRMYVTAVRDI 300 310 320 330 340 350 370 m922. ep ANSLGGPGLX g922 ANSLGGPGLX 360 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2817>: a922.seq 1 ATGAAAAACA GAAAAATACT GCCGCTGGCA ATTTGTTTGG CGGCTTTGTC 51 TGCCTGTACG GCGATGGAGG CACGCCCGCC CCGGGCAAAT GAAGCCCAAG 101 CCCCCCGCGC GGATGAAATG AAAAAAGAAA GCCGCCCCGC GTTTGACGCG 151 GCAGCCGTAT TTGACGCGGC AGCCGTACCG GTATCCGACA GCGGGTTTGC 201 CGCCAATGCA AATGTCCGCC GTTTTGTGGA CGATGAAGTC GGGAAAGGGG 251 ATTTTTCCCG GGCGGAATGG CAGGATTTTT TTGACAAAGC GGCTTACAAG 301 GCGGACATCG TCAAGATTAT GCACCGCCCC TCCACATCGC GTCCGTGGTA 351 TGTGTTCCGC ACGGGAAATT CGGGCAAGGC GAAATTTCGC GGCGCGCGCC 401 GGTTTTATGC GGAAAACCGC GCGCTTATCG ATGATGTGGC GCAAAAATAC 451 GGCGTGCCTG CCGAACTTAT CGTGGCGGTT ATCGGGATTG AAACGAATTA 501 CGGCAAAAAT ACGGGCAGTT TCCGTGTGGC GGACGCATTG GCGACCTTAG 551 GCTTTGATTA CCCCCGCCGC GCCGGGTTTT TCCAAAAAGA ATTGGTCGAG 601 CTTTTAAAGC TGGCAAAAGA AGAAGGCGGC GATGTTTTCG CCTTTAAAGG 651 CAGCTATGCG GGCGCAATGG GGATGCCGCA ATTTATGCCT TCGAGCTACC 701 GGAAATGGGC GGTGGATTAT GACGGGGACG GACATCGGGA CATATGGGGC 751 AATGTTGGCG ATGTCGCGGC ATCGATTGCC AATTATATGA AGCAGCACGG 801 TTGGCGCACG GGCGGGAAAA TACTGGTGTC TGCAACATTG GCGCCGGGTG 851 CGGATGTTCA GGCAATCATT GGCGAAAAAA CCGCCCTGAC GCGGACGGTG 901 GCGGATTTGA AGGCGTACGG CATCATCCCC GGCGAAGAGC TTGCCGATGA 951 TGAAAAGGCG GTTTTGTTCA AACTGGAAAC CGCACCCGGC GTGTTTGAAT 1001 ATTATTTGGG CTTGAACAAT TTTTATACGG TATGGCAGTA CAATCACAGT 1051 CGGATGTATG TAACGGCGGT CAGGGACATT GCCAATTCGC TTGGCGGCCC 1101 GGGATTGTAA This corresponds to the amino acid sequence <SEQ ID 2818; ORF 922.a>: a922.pep 1 MKΝRKILPLA ICLAALSACT AMEARPPRAΝ EAQAPRADEM KKESRPAFDA 51 AAVFDAAAVP VSDSGFAAΝA ΝVRRFVDDEV GKGDFSRAEW QDFFDKAAYK 101 ADIVKIMHRP STSRPWYVFR TGΝSGKAKFR GARRFYAEΝR ALIDDVAQKY 151 GVPAELIVAV IGIETΝYGKΝ TGSFRVADAL ATLGFDYPRR AGFFQKELVE 201 LLKLAKEEGG DVFAFKGSYA GAMGMPQFMP SSYRKWAVDY DGDGHRDIWG 251 ΝVGDVAASIA ΝYMKQHGWRT GGKILVSATL APGADVQAII GEKTALTRTV 301 ADLKAYGIIP GEELADDEKA VLFKLETAPG VFEYYLGLΝΝ FYTVWQYΝHS 351 RMYVTAVRDI AΝSLGGPGL* m922/a922 98.9% identity in 369 aa overlap 10 20 30 40 50 60 m922. pep MKKRKILPLAICLAALSACTAMEARPPRAΝEAQAPRAVEMKKESRPAFDAAAVFDAAAVP I Ml II I I I II I I II II I I II II II I II II II II II I I II II II II II II II I II I II I a922 MKΝRKILPLAICLAALSACTAMEARPPRAΝEAQAPRADEMKKESRPAFDAAAVFDAAAVP 10 20 30 40 50 60 70 80 90 100 110 120 m922.pep VSDSGFAAΝAΝVRRFVDDEVGKGDFSRAEWQDFFDKAAYKADIVKIMHRPSTSRPWYVFR II II I II II II II II II I I II II I II II I I II I II I II I I I II II I I II II II I II I II I a922 VSDSGFAAΝAΝVRRFVDDEVGKGDFSRAEWQDFFDKAAYKADIVKIMHRPSTSRPWYVFR 70 80 90 100 110 120 130 140 150 160 170 180 m922. pep TGΝSGKAKFRGARRFYAEΝRALIDDVAQKYGVPAELIVAVIGIETΝYGKΝTGSFRVADAL II I I I II II II II II I II II II I II II I I II II I II II II I I I II I I I I I II II I II II I a922 TGΝSGKAKFRGARRFYAEΝRALIDDVAQKYGVPAELIVAVIGIETΝYGKΝTGSFRVADAL 130 140 150 160 170 180 190 200 210 220 230 240 m922. pep ATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGSYAGAMGMPQFMPSSYRKWAVDY II II II II II I II I I II II II II I II I I II I I II I II II I II II I I I II I I I I II I I II I a922 ATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGSYAGAMGMPQFMPSSYRKWAVDY 190 200 210 220 230 240 250 260 270 280 290 300 m922. pep DGDGHRDIWGNVGDVAASVANYMKQHGWRTGGKMLVSATLAPGADVQAIIGEKTALTRTV I II I II I II II II I I II I: I II II II I II II I I M II II I I II II I II II II II II II I I a922 DGDGHRDIWGNVGDVAASIANYMKQHGWRTGGKILVSATLAPGADVQAIIGEKTALTRTV 250 260 270 280 290 300 310 320 330 340 350 360 m922. pep ADLKAYGIIPGEELADDEKAVLFKLETAPGVFEYYLGLNNFYTVWQYNHSRMYVTAVRDI I II II I II I II II II II II I II I II II I II I I II I I I I II I II II II I II II II I II II I a922 ADLKAYGIIPGEELADDEKAVLFKLETAPGVFEYYLGLNNFYTVWQYNHSRMYVTAVRDI 310 320 330 340 350 360 370 m922.pep ANSLGGPGLX I I I II I I I II a922 ANSLGGPGLX 370 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 2819>: g923.seq 1 ATGAAGCGGC AGGCTTTCTT CAAACCGATG GCGTGTGCGG CATTTCTGTC 51 CGCCGTTTCG CTGCGCCTCC CCGTATTGGG CGCGTGTTAC GCAATATTGT 101 CCCTCTATGC GTTTGCACTT TACGGCATCG ACAAACGGCG TGCCGTGCGG 151 GGAAAACGCC GCATTCCCGA ACACCGCCTG CTCCTGCCTG CCTTGTTCGG 201 CGGTTGGACG GGCGCATACT TGGGTAGTAG GATGTTCAGG CATAAAACGG 251 CGAAAAAGCG TTTTGTTGTG CTGTTCCGTC TGACTGTTTC GGGCAATGTC 301 CTGGCGACCT GCATCCTGAT TGATTATTTC GTTCCGCCCG AACTTTTTGT 351 AAAACTCGGG CAACATCTCT GA This corresponds to the amino acid sequence <SEQ ID 2820; ORF 923. ng>: g923.pep 1 MKRQAFFKPM ACAAFLSAVS LRLPVLGACY AILSLYAFAL YGIDKRRAVR 51 GKRRIPEHRL LLPALFGGWT GAYLGSRMFR HKTAKKRFW LFRLTVSGΝV 101 LATCILIDYF VPPELFVKLG QHL* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2821>: m923.seq 1 ATGAAGCGGC AGGCTTTCTT CAAACTGATG GCGTGTGCGG CATTTCTGTC 51 TGCCGTTTCG CTGCGCCTCC CCGTATTGGG CGCGTGTTAC GCAATATTGT 101 CCCTCTATGC GTTTGCACTT TACGGCATCG ACAAACGGTG CGCCATACGG 151 GGGCAACGCC GCATTCCCGA ACACCGCCTG CTGCTGCCTG CATTGCTCGG 201 CGGCTGGGTG GGCGCGTATT TCGGCAGCAT GACATTCAAA CATAAGACAG 251 CGAAAAAGCG TTTTGTTGTG CTGTTCCGTC TGACTGTTTC AGGTAATGTC 301 TTGGCGACCC TCATCCTGAT TTATAGTGGA TTAAATTTAA ACCAGTACGG 351 CGTTGCCTCG CCTTGCCGTA CTATTTGTAC TGTCTGCGGC TTCGTCGCCT 401 TGTCCTGATT TTTGTTAATC CACTATAT . T ATTTTGTCCC GCCTGAATTT 451 TTCGTAAAAC TCGGGCAGAA TACCTGA This corresponds to the amino acid sequence <SEQ ID 2822; ORF 923>: m923.pep 1 MKRQAFFKLM ACAAFLSAVS LRLPVLGACY AILSLYAFAL YGIDKRCAIR 51 GQRRIPEHRL LLPALLGGWV GAYFGSMTFK HKTAKKRFW LFRLTVSGΝV 101 LATLILIYSG LΝLΝQYGVAS PCRTICTVCG FVALS*FLLI HYXYFVPPEF 151 FVKLGQΝT* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 923 shows 68.8% identity over a 157 aa overlap with a predicted ORF (ORF 923.ng) from N gonorrhoeae: g923/m923 10 20 30 40 50 60 g923.pep MKR Q AFFKPMACAAFLSAVSLRLPVLGACYAILSLYAFALYGIDKRRAVRGKRRIPEHRL I I I I I M I I M M M M M M M M M M M M M M I M M M I m923 MKRQAFFKLMACAAFLSAVSLRLPVLGACYAILSLYAFALYGIDKRCAIRGQRRIPEHRL 10 20 30 40 50 60 70 80 90 100 g923. pep LLPALFGGWTGAYLGSRMFRHKTAKKRFWLFRLTVSGΝVLATCILID m923 LLPALLGGWVGAYFGSMTFKHKTAKKRFWLFRLTVSGΝVLATLILIYSGLΝLΝQYGVAS 70 80 90 100 110 120 110 120 g923. pep YFVPPELFVKLGQHLX l l l l l h l l l l l h m923 PCRTICTVCGFVALSXFLLIHYIYFVPPEFFVKLGQΝTX 130 140 150 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2823>: a923.seq 1 ATGAAGCGGC AGGCTTTCTT CAAACTGATG GCGTGTGCGG CATTTCTGTC 51 TGCCGTTTCG CTGCGCCTCC CCGTATTGGG CGCGTGTTAC GCAATATTGT 101 CCCTCTATGC GTTTGCACTT TACGGCATCG ACAAACGGCG TGCCGTGCGG 151 GGAAAACGCC GCATTCCCGA ACACCGCCTG CTGCTGCCTG CCTTGTTCGG 201 CGGTTGGGCG GGCGCATACT TGGGCAGCAG GATATTCAGG CATAAAACGG 251 CGAAAAAGCG TTTTGTTGTG CTGTTCCGTC TGACTGTTTC GGGCAATGTC 301 CTGGCGACCC TCATCCTGAT TTATAGTGGA TTAAATTTAA ACCAGTACGG 351 CGTTGCCTCG CCTTA.GCTC AAAGAGAACG ATTCTCTAAG GTGCTGAAGC 401 ACCAAGTGAA TCGGTTCCGT ACTATTTGTA CTGTCTGCGG CTTCGTCGCC 451 TTGTCCTGAT TTTTGTTAAT CCACTAT.AT TATTTTGTCC CGCCTGAATT 501 TTTCGTAAAA CTCGGGCAGA ATACCTGA This corresponds to the amino acid sequence <SEQ ID 2824; ORF 923. a>: a923.pep 1 MKRQAFFKLM ACAAFLSAVS LRLPVLGACY AILSLYAFAL YGIDKRRAVR 51 GKRRIPEHRL LLPALFGGWA GAYLGSRIFR HKTAKKRFW LFRLTVSGΝV 101 LATLILIYSG LΝLΝQYGVAS PXAQRERFSK VLKHQVΝRFR TICTVCGFVA 151 LS*FLLIHYX YFVPPEFFVK LGQΝT* m923/a923 84.6% identity in 175 aa overlap 10 20 30 40 50 60 m923. pep MKRQAFFKLMACAAFLSAVSLRLPVLGACYAILSLYAFALYGIDKRCAIRGQRRIPEHRL I II I II I II II I I II I II II I II I II I I II II II II I I II II II II I: I I: I I II II II a923 MKRQAFFKLMACAAFLSAVSLRLPVLGACYAILSLYAFALYGIDKRRAVRGKRRIPEHRL 10 20 30 40 50 60 70 80 90 100 110 120 m923.pep LLPALLGGWVGAYFGSMTFKHKTAKKRFWLFRLTVSGΝVLATLILIYSGLΝLΝQYGVAS I I II IM MM I Ml I M M II II I I II II II II II II I II I M I II II I II I I II II a923 LLPALFGGWAGAYLGSRIFRHKTAKKRFWLFRLTVSGΝVLATLILIYSGLΝLΝQYGVAS 70 80 90 100 110 120 130 140 150 159 m923.pep PC RTICTVCGFVALSXFLLIHYXYFVPPEFFVKLGQΝTX I I I I I M II II II I II II II II llll II II I II I II II a923 PXAQRERFSKVLKH Q VΝRFRTICTVCGFVALSXFLLIHYXYFVPPEFFVKLGQΝTX 130 140 150 160 170 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2825>: g925.seq 1 ATGAAACAAA TGCTTTTGGC cgtcggcgtg ggcGCGGTGT TGGCGGGCTG 51 CGGCAaggat gcCGGCGGtt acgagggtTA TTGGCGCGAA AAGTCGGACA 101 AAAAagaggG CGTGATTGCC GTCAAAAAAA AAGGCAATTA CTTCCTTAAT 151 AAAATCAACG TGTTTACAGG CAAGGAGGAG TCTTTGCTTT TGTCTGAAAA 201 AGACGGCGCG CTTTCGATAA ACACGGGGAT AGGGGAAATC CCGATCAAAC 251 TTTCCGACGA CGGGAAAGAG CTGTATGTCG AACGCAGGCG GTATGTGAAA 301 ACCGATGCGG CGATGAAGGA CAAAATCATC GCCCACCAGA AAAAGTGCGG 351 ACAAACGGCA CAGGCATACC TCGACGCGCG AAATGCGTTG CCGTCAAACC 401 AAACGTATCA GCAGCGTCAG GCGGCGATCG AGCAATTGAA ACGGCGGTTT 451 GAAGCCGAGT TTGACGAATT GGAAAAAGAA ATCAAATGCA ACGGCAAACC 501 GACATTGTTG TTTTAG This corresponds to the amino acid sequence <SEQ ID 2826; ORF 925. ng>: g925.pep 1 MKQMLLAVGV GAVLAGCGKD AGGYEGYWRE KSDKKEGVIA VKKKGΝYFLΝ 51 KIΝVFTGKEE SLLLSEKDGA LSIΝTGIGEI PIKLSDDGKE LYVERRRYVK 101 TDAAMKDKII AHQKKCGQTA QAYLDARΝAL PSΝQTYQQRQ AAIEQLKRRF 151 EAEFDELEKE IKCΝGKPTLL F* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2827>: m925.seq (partial) 1 ATGAAACAAA TGCTTTTAGC CGTCGGCGTG GTGGCGGTGT TGGCGGGCTG 51 CGGCAAGGAT GCCGGCGGTT ACGAGGGTTA TTGGCGCGAA AAGTCGGACA 101 AAAAAGAGGG TATGATTGCC GTCAAAAAAG AAAAAGGCAA TTACTTCCTT This corresponds to the amino acid sequence <SEQ ID 2828; ORF 925>: m925.pep (partial) 1 MKQMLLAVGV VAVLAGCGKD AGGYEGYWRE KSDKKEGMIA VKKEKGΝYFL.. Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 925 shows 94.0% identity over a 50 aa overlap with a predicted ORF (ORF 925.ng) from N gonorrhoeae: m925/g925 10 20 30 40 50 m925. pep MKQMLLAVGWAVLAGCGKDAGGYEGYWREKSDKKEGMIAVKKEKGΝYFL I M 11111 II 11 M 11111111 M I h 11111 MMM g925 MKQMLLAVGVGAVLAGCGKDAGGYEGYWREKSDKKEGVIAVKK- KGΝYFLΝKIΝVFTGKE 10 20 30 40 50 g925 ESLLLSEKDGALSIΝTGIGEIPIKLSDDGKELYVERRRYVKTDAAMKDKIIAHQKKCGQT 60 70 80 90 100 110 The following partial DΝA sequence was identified in N. gonorrhoeae <SEQ ID 2829>: g925-l.seq 1 ATGAAACAAA TGCTTTTGGC CGTCGGCGTG GCGGCGGTGT TGGCGGGCTG 51 CGGCAAGGAT GCCGGCGGTT ACGAGGGTTA TTGGCGCGAA AAGTCGGACA 101 AAAAAGAGGG CGTGATTGCC GTCAAAAAAA AAGGCAATTA CTTCCTTAAT 151 AAAATCAACG TGTTTACAGG CAAGGAGGAG TCTTTGCTTT TGTCTGAAAA 201 AGACGGCGCG CTTTCGATAA ACACGGGGAT AGGGGAAATC CCGATCAAAC 251 TTTCCGACGA CGGGAAAGAG CTGTATGTCG AACGCAGGCG GTATGTGAAA 301 ACCGATGCGG CGATGAAGGA CAAAATCATC GCCCACCAGA AAAAGTGCGG 351 ACAAACGGCA CAGGCATACC TCGACGCGCG AAATGCGTTG CCGTCAAACC 401 AAACGTATCA GCAGCGTCAG GCGGCGATCG AGCAATTGAA ACGGCGGTTT 451 GAAGCCGAGT TTGACGAATT GGAAAAAGAA ATCAAATGCA ACGGCAAACC 501 GACATTGTTG TTTTAG This corresponds to the amino acid sequence <SEQ ID 2830; ORF 925-1. ng>: g925-l .pep 1 MKQMLLAVGV AAVLAGCGKD AGGYEGYWRE KSDKKEGVIA VKKKGNYFLN 51 KINVFTGKEE SLLLSEKDGA LSINTGIGEI PIKLSDDGKE LYVERRRYVK 101 TDAAMKDKII AHQKKCGQTA QAYLDARNAL PSNQTYQQRQ AAIEQLKRRF 151 EAEFDELEKE IKCNGKPTLL F* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2831>: m925-l.seq 1 ATGAAACAAA TGCTTTTAGC CGTCGGCGTG GTGGCGGTGT TGGCGGGCTG 51 CGGCAAGGAT GCCGGCGGTT ACGAGGGTTA TTGGCGCGAA AAGTCGGACA 101 AAAAAGAGGG TATGATTGCC GTCAAAAAAG AAAAAGGCAA TTACTTCCTT 151 AATAAAATCC ACGTGGTTAC AGGCAAGGAA GAGTCCTTGC TTTTGTCTGA 201 AAAAGACGGC GCGCTTTCGA TAAACACAGG GATAGGGGAA ATCCCGATCA 251 AACTTTCCGA CGACGGGAAA GAGCTGTATG TCGAACGTAG GCAGTATGTC 301 AAAACCGATG CGGCGATGAA GGACAAAATC ATCGCCCATC AGAAAAAGTG 351 CGGACAAACA GCACAGGCAT ACCGCGACGC GCGAAATGCG TTGCCGTCAA 401 ACCAGACGTA TCAGCAGCAT CTGGCGGCGA TCGAGCAATT GAAACGGCGG 451 TTTGAAGCCG AGTTTGACGA ATTGGAAAAA GAAATCAAAT GCAACGGCAG 501 AAGCCCGGCA TTGTTGCTTT AG This corresponds to the amino acid sequence <SEQ ID 2832; ORF 925-l>: m925-l.pep.. 1 MKQMLLAVGV VAVLAGCGKD AGGYEGYWRE KSDKKEGMIA VKKEKGΝYFL 51 ΝKIHVVTGKE ESLLLSEKDG ALSIΝTGIGE IPIKLSDDGK ELYVERRQYV 101 KTDAAMKDKI IAHQKKCGQT AQAYRDARΝA LPSΝQTYQQH LAAIEQLKRR 151 FEAEFDELEK EIKCΝGRΞPA LLL* m925/g925 92.5% identity in 173 aa overlap 10 20 30 40 50 60 m925-l . pep MKQMLLAVGVVAVLAGCGKDAGGYEGYWREKSDKKEGMIAVKKEKGΝYFLΝKIHVVTGKE I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I II I I I : I llll g925-l MKQMLLAVGVAAVLAGCGKDAGGYEGYWREKSDKKEGVIAVKK-KGΝYFLΝKIΝVFTGKE 10 20 30 40 50 70 80 90 100 110 120 m925-l.pep ESLLLSEKDGALSIΝTGIGEIPIKLSDDGKELYVERRQYVKTDAAMKDKIIAHQKKCGQT I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I II II I : II I I I I I I II I II I I I I I I I II g925-l ESLLLSEKDGALSIΝTGIGEIPIKLSDDGKELYVERRRYVKTDAAMKDKIIAHQKKCGQT 60 70 80 90 100 110 130 140 150 160 170 m925-l.pep AQAYRDARΝALPSΝQTYQQHLAAIEQLKRRFEAEFDELEKEIKCΝGRSPALLLX MM II I I I I I I I II I I I : I II I I II I I I I I I I I I I I I I I I I I I : MUM g925-l AQAYLDARΝALPSΝQTYQQRQAAIEQLKRRFEAEFDELEKEIKCΝGK-PTLLFX 120 130 140 150 160 170 The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 2833>: a925-l.seq 1 AATAAAATCA ACGTGTTTAC AGGTAAGGAA GAATCTATGC TTTTGTCTGA 51 AAAAGACGGC GCGCTTTCGA TAAACACGGG GATAGGGGAA ATCCCGATCA 101 AACTTTCCGA CGACGGGAAA GAGCTGTATG TCGAACGCAG GCAGTATGTC 151 AAAACCGATG CGGCGATGAA GGACAAAATC ATCGCCCATC AGAAAAAGTG 201 CGGACAAACG GCACAGGCAT ATCTCGACGC GCGAAATGCG TTGCCGTCAA 251 ACCAGACGTA TCAGCAGCAT CAGGCGGCGA TCGAGCAGTT GAAACGGCGG 301 TTTGAAGCCG AGTTTGACGA ATTGGAAAAA GAAATCAAAT GCAACGGCAA 351 ACCGACATTG TTGTTTTAG This corresponds to the amino acid sequence <SEQ ID 2834; ORF 925-l.a>: a925-l.pep 1 ΝKIΝVFTGKE ESMLLSEKDG ALSIΝTGIGE IPIKLSDDGK ELYVERRQYV 51 KTDAAMKDKI IAHQKKCGQT AQAYLDARΝA LPSΝQTYQQH QAAIEQLKRR 101 FEAEFDELEK EIKCΝGKPTL LF* a925-l/m925-l 92.7% identity in 123 aa overlap 10 20 30 a925-l.pep ΝKIΝVFTGKEESMLLSEKDGALSIΝTGIGE II M I I I I I I M I I II M I I I I I I I I I I I m925-l AGGYEGYWREKSDKKEGMIAVKKEKGΝYFLΝKIHVVTGKEEΞLLLSEKDGALSIΝTGIGE 30 40 50 60 70 80 40 50 60 70 80 90 a925-l.pep IPIKLΞDDGKELYVERRQYVKTDAAMKDKIIAHQKKCGQTAQAYLDARNALPΞNQTYQQH I I I I I I I I I I I I I II I I I II II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I M I m925-l IPIKLSDDGKELYVERRQYVKTDAAMKDKIIAHQKKCGQTAQAYRDARNALPSNQTYQQH 90 100 110 120 130 140 100 110 120 a925-l .pep QAAIEQLKRRFEAEFDELEKEIKCNGK-PTLLFX I I I I I I I II I I I I II I I I I I I I I I M MMM m925-l LAAIEQLKRRFEAEFDELEKEIKCNGRSPALLLX 150 160 170 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2835>: g926.seq (partial) 1 ATGAAACACA CCGTATCCGC ATCGGTCATC CTGCTTTTGA CCGCTTGCGC 51 GCAATTACCT CAAAATAACG AAAACCTGTG GCAGCCGTCC GAACACATCA 101 GCAGTTTTGC AGCGGAAGGG CGGTTGGCAG TCAAAGCGGA AGGGAAAGGT 151 TCGTATGCAA ATTTCGATTG GACATACCAA CCGCCCGTGG AAACCATCAA 201 TATCAACACC CCTTTGGGCA GTACGCTCGG ACAGTTGTGT CAAGacAGGG 251 ACGGCGCATT GGCAGTGGAC GGCAAAGGAA ATGTCTATCA GGCAGAGGGT 301 ACGgaagact tGAGCAGGCA GCTGGTCGGT TTCAAACTGC CAATCCAATA 351 TCTGCATATC TGGGCGGAAG GCAGGCGTGT GGCGGGCGCG CCTtaccGCA 401 TCCGTTCAGA CGGCATATTG GAACAATAcg GttggACAAT cgggCagaac 451 tgcCGACAGT GGGGGGCaag tccgaacgtt gcaactGAa ... This corresponds to the amino acid sequence <SEQ ID 2836; ORF 926.ng>: g926.pep (partial) 1 MKHTVSASVI LLLTACAQLP QΝΝEΝLWQPS EHISSFAAEG RLAVKAEGKG 51 SYAΝFDWTYQ PPVETIΝIΝT PLGSTLGQLC QDRDGALAVD GKGΝVYQAEG 101 TEDLSRQLVG FKLPIQYLHI WAEGRRVAGA PYRIRSDGIL EQYGWTIGQΝ 151 CRQWGAΞPΝV ATE... The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2837>: m926.seq 1 ATGAAACACA CCGTATCCGC ATCGGTCATC CTGCTTTTGA CCGCTTGCGC 51 GCAATTACCT CAAAATAACG AAAACCTGTG GCAGCCGTCC GAACACATCA 101 GCAGTTTTGC AGCAGAAGGG CGGTTGGCAG TGAAAGCGGA AGGGAAAGGT 151 TCGTATGCAA ATTTCGATTG GACATACCAA CCGCCCGTGG AAACCATCAA 201 TATCAATACC CCTTTGGGCA GTACGCTCGG GCAGTTGTGT CAAGACAGGG 251 ACGGCGCATT GGCAGTGGAC GGCAAAGGAA ATGTCTATCA GGCGGAAAGT 301 GCGGAAGAAT TGAGCAGGCA GCTGGTCGGT TTCAAACTGC CAATCCAATA 351 TCTGCATATC TGGGCAGATG GCAGGCGTGT GGCGGGCGCG CCTTACCGCA 401 TCCTGCCGGA CGGCATATTG GAACAATACG GTTGGACTGT CGGCAGAACC 451 GCCGACAGTG GGGGGCAAGT CCGAACGTTG CAACTGAATA ACGGAAATTT 501 GAACATCAGG CTGGTTTTCA CCGAAATCGG TATGCCGTCT GAAACCGAAA 551 CCCCGGAACG CTGTGCGGCG CGCACGAGAT AA This corresponds to the amino acid sequence <SEQ ID 2838; ORF 926>: m926.pep 1 MKHTVSASVI LLLTACAQLP QΝΝEΝLWQPS EHISSFAAEG RLAVKAEGKG 51 SYAΝFDWTYQ PPVETIΝIΝT PLGSTLGQLC QDRDGALAVD GKGΝVYQAES 101 AEELSRQLVG FKLPIQYLHI WADGRRVAGA PYRILPDGIL EQYGWTVGRT 151 ADSGGQVRTL QLΝΝGΝLΝIR LVFTEIGMPS ETETPERCAA RTR* g926/m926 91.6% identity in 155 aa overlap 10 20 30 40 50 60 g926.pep MKHTVSASVILLLTACAQLPQΝΝEΝLWQPSEHISSFAAEGRLAVKAEGKGSYAΝFDWTYQ 111111 ! ! I I M M i I i 11 M II M 1119 E 1111 II 11111 i 1111 M I i E I f 11 ! M 11 m926 MKHTVSASVILLLTACAQLPQΝΝEΝLWQPSEHISSFAAEGRLAVKAEGKGSYAΝFDWTYQ 10 20 30 40 50 SO 70 80 90 100 110 120 g926.pep PPVETIΝIΝTPLGSTLGQLCQDRDGALAVDGKGΝVYQAEGTEDLSRQLVGFKLPIQYLHI I M I I H I I I I M I I M M M M M I I I M M M M I I h M M M I M I I I I M I I M I m92S PPVETIΝIΝTPLGΞTLGQLCQDRDGALAVDGKGΝVYQAESAEELSRQLVGFKLPIQYLHI 70 80 90 100 110 120 130 140 150 160 g926.pep WAEGRRVAGAPYRIRSDGILEQYGWTIGQΝCRQWGASPΝVATE 1111111111 = 1- = I m926 WADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTLQLNNGNLNIRLVFTEIGMPS 130 140 150 160 170 180 a926. seq 1 ATGAAACACA CTGTATCCGC ATCGGTCATC CTGCTTTTGA CCGCTTGCGC 51 GCAATTACCT CAAAATAACG AAAACCTGTG GCAGCCGTCC GAACACACCC 101 GCAGTTTCAC GGCGGAAGGG CGGTTGGCAG TGAAAGCGGA AGGGAAAGGT 151 TCGTATGCAA ATTTCGATTG GACATACCAA CCGCCCGTGG AAACCATCAA 201 TATCAACACC CCTTTGGGCA GTACGCTCGG GCAGTTGTGT CAAGACAGGG 251 ACGGCGCATT GGCAGTGGAC GGCAAAGGAA ATGTCTATCA GGCGGAAAGT 301 GCGGAAGAAT TGAGCAGGCA GCTGGTCGGT TTCAAACTGC CAATCCAATA 351 TCTGCATATC TGGGCAGATG GCAGGCCTGT GGCGGGCGCG CCTTACCGCA 401 TCCTGCCGGA CGGCATATTG GAACAATACG GTTGGACTGT CGGCAGAACC 451 GCCGACAGTG GGGGGCAAGT CCGAACGTTG CAACTGAATA ACGGAAATTT 501 GAACATCAGG CTGGTTTTCA CCGAGATTGG TATGCCGTCT GAAACCGAAA 551 CCCAAGAACA ATGCGCGGCA CGCATACAGT AA a926.pep 1 MKHTVSASVI LLLTACAQLP QNNENLWQPS EHTRSFTAEG RLAVKAEGKG 51 SYANFDWTYQ PPVETININT PLGSTLGQLC QDRDGALAVD GKGNVYQAES 101 AEELSRQLVG FKLPIQYLHI WADGRPVAGA PYRILPDGIL EQYGWTVGRT 151 ADSGGQVRTL QLNNGNLNIR LVFTEIGMPS ETETQEQCAA RIQ* m926/a926 96.9% identity in 191 aa overiap 10 20 30 40 50 60 m926. pep MKHTVSASVILLLTACAQLPQNNENLWQPSEHISSFAAEGRLAVKAEGKGSYANFDWTYQ I I II II I II II II I II I II I I II II II I I I II I M I II I II I I I II I II II I II I II I a926 MKHTVSASVILLLTACAQLPQNNENLWQPΞEHTRSFTAEGRLAVKAEGKGSYANFDWTYQ 10 20 30 40 50 60 70 80 90 100 110 120 m926.pep PPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAEELSRQLVGFKLPIQYLHI I I II I I I I II II II II II II II II I II I I II I I I I I I II I I II I I I I I II I II I II I II I a926 PPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAEELSRQLVGFKLPIQYLHI 70 80 90 100 110 120 130 140 150 160 170 180 m926.pep WADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTLQLNNGNLNIRLVFTEIGMPS II I II I II II II II II II I II II I I I II II II II II I II I I I II I II I II II I II I I I I a926 WADGRPVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTLQLNNGNLNIRLVFTEIGMPS 130 140 150 160 170 180 190 m926.pep ETETPERCAARTRX llll MMM a926 ETETQEQCAARIQX 190 The following partial DNA sequence was identified in N.gonorrhoeae <SEQ ID 2839>: g927.seq 1 atgaaaacct acGCAcAggC ACTCTATacc GCAGCCCTGC TCACCGCCTG 51 CAGCCCcgca GCcgatTcaa accaTCCGTC CGGAcAaAAT GCCCCGGCCA 101 ATACCGAATC cgacGgaaAA AACATtaccC TGctcaatgc cTcgtacgat 151 gtGACACGGT ATTTttacaa agaatacgac cacTtgtttg tcggaaCATA 201 CCAATCCGAA CACCCCGGCA CATCCGTCAG CATCCAACAA TCCCACGGCG 251 GCTTCAGCAA ACAGGCATTA TCCGTAGCCA ACGGCCTTCA AGCCGATGTC 301 GTAACCATGA ACCAATCTTC CGACATCGAC CTGCTCGAAA AAAA.GGACT 351 GGTAGAAAAA GGCTGGCAAC AAGCCCTCCC CGATCACGCC GCACCCTACA 401 CCAGCACTAT GGTTTTCCTT GTCCGAAAAA ACAACCCcaa ACAGAtccgC 451 GATTGGAACG ACCTTGCCAA AGACGGCGTT AACATCGTCA TCGCCAAGAC 501 CTCGGGCAAC GGACGCTACG CCTTCCTCGG CGCATACGGT TACGGTCTGA 551 AAGCCAACAA CGGcaaCGAG CAGGAAGCCC AAAAACTCGT CGCATCCATC 601 CTCAAAAACA CACCCGTTTT TGAAAACGGC GGACGCGc . C CGCCGCCACC 651 ACCTTCACAC AACGCAACAT CGGCGACGTA CTCATCACTT TTGAAAACga 701 agCcaactac gtCAGCAAAA AACTGA This corresponds to the amino acid sequence <SEQ ID 2840; ORF 927.ng>: g927.pep 1 MKTYAQALYT AALLTACSPA ADSNHPSGQN APANTESDGK NITLLNASYD 51 VTRYFYKEYD HLFVGTYQSE HPGTSVSIQQ SHGGFSKQAL SVANGLQADV 101 VTMNQSSDID LLEKXGLVEK GWQQALPDHA APYTSTMVFL VRKNNPKQIR 151 DWNDLAKDGV NIVIAKTSGN GRYAFLGAYG YGLKANNGNE QEAQKLVASI 201 LKNTPVFENG GRXPPPPPSH NATSATYSSL LKTKPTTSAK N* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2841>: m927.seq 1 ATGAAAACCT ACGCACCGGC ACTCTATACC GCAGCCCTGC TCACCGCCTG 51 CAGCCCCGCA GCCGATTCAA ACCATCCGTC CGGACAAAAT GCCCCGGCCA 101 ATACCGAATC CGACGGAAAA AACATTACCC TGCTCAACGC CTCATACGAT 151 GTGGCACGGG ATTTTTACAA AGAATACAAC CCCTTATTTA TCAAAACATA 201 CCAATCCGAA CACCCCGGCA CATCCGTCAG CATCCAACAG TCCCACGGCG 251 GCTCCAGCAA ACAGGCATTA TCCGTAGCCA ACGGCCTTCA AGCCGATGTC 301 GTAACCATGA ACCAATCCTC CGACATCGAC CTGCTCGAAA AAAAAGGACT 351 GGTAGAAAAA GGCTGGCAAC AAGCCCTCCC CGACCACGCC GCGCCCTACA 401 CCAGCACTAT GGTTTTCCTT GTCCGAAAAA ACAACCCCAA ACAGATCCGC 451 GATTGGAACG ACCTTGCCAA AGACGGCGTT AACATCGTCA TCGCCAATCC 501 CAAAACCTCG GGCAACGGAC GCTACGCCTT CCTCGGCGCA TACGGTTACG 551 GTCTGAAAAC CACCAACGGC AACGAACAGG AAGCCCAAAA ACTCGTCGCA 601 TCCATCCTCA AAAACACCCC CGTTTTTGAA AACGGCGGAC GCkCgCCACC 651 ACCACCTTCA CACAACGCAA CATCGGCGAC GTACTCATCA CTTTTGAAAA 701 CGAAGCCAAC TACGTCAGCr AAAAACtGA This corresponds to the amino acid sequence <SEQ ID 2842; ORF 927>: m927.pep 1 MKTYAPALYT AALLTACSPA ADSNHPSGQN APANTESDGK NITLLNASYD 51 VARDFYKEYN PLFIKTYQSE HPGTSVSIQQ SHGGSSKQAL SVANGLQADV 101 VTMNQSSDID LLEKKGLVEK GWQQALPDHA APYTSTMVFL VRKNNPKQIR 151 DWNDLAKDGV NIVIANPKTS GNGRYAFLGA YGYGLKTTNG NEQEAQKLVA 201 SILKNTPVFE NGGRXPPPPS HNATSATYSS LLKTKPTTSA KN* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 927 shows 94.2% identity over a 243 aa overlap with a predicted ORF (ORF 927.ng) from N gonorrhoeae: g927/m927 10 20 30 40 50 60 g927.pep MKTYAQALYTAALLTACSPAADSΝHPSGQΝAPAΝTESDGKΝITLLΝASYDVTRYFYKEYD M i l l M M M M M M M M M M M M M M M M M M I M M M I h l l l l l h m927 MKTYAPALYTAALLTACSPAADSΝHPSGQΝAPAΝTESDGKΝITLLΝASYDVARDFYKEYΝ 10 20 30 40 50 60 70 80 90 100 110 120 g927.pep HLFVGTYQSEHPGTSVSIQQSHGGFSKQALSVAΝGLQADWTMΝQSSDIDLLEKXGLVEK 1 1 = M M M M M I M M M M I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M i l l m927 PLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVAΝGLQADWTMΝQSSDIDLLEKKGLVEK 70 80 90 100 110 120 130 140 150 160 170 g927.pep GWQQALPDHAAPYTSTMVFLVRKΝΝPKQIRDWΝDLAKDGVΝIVIA- - KTSGΝGRYAFLGA MMMMMIMMMMMMMMMMMIMIMMMM MIMMMMM m927 GWQQALPDHAAPYTSTMVFLVRKΝΝPKQIRDWΝDLAKDGVΝIVIAΝPKTSGΝGRYAFLGA 130 140 150 160 170 180 180 190 200 210 220 230 g927.pep YGYGLKANNGNEQEAQKLVASILKNTPVFENGGRXPPPPPSHNATSATYSSLLKTKPTTS 1 1 1 1 1 : 1 1 1 1 1 M 1 1 1 M i l l I I I M M I M M M M I I M M M M M M M M M m927 YGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRXPPPP-SHNATSATYSSLLKTKPTTS 190 200 210 220 230 240 g927.pep AKNX m927 AKNX 240 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2843>: a927.seq 1 ATGAAAACCT ACGCACCGGC ACTCTATACC GCAGCCCTGC TCAGCGCCTG 51 CAGCCCCGCA GCCGATTCAA ACCATCCGTC CGGACAAAAT GCCCCGGCCA 101 ATACCGAATC CGACGGAAAA AACATTACCC TGCTCAACGC CTCATACGAT 151 GTGGCACGGG ATTTTTACAA AGAATACAAC CCCTTATTTA TCAAAACATA 201 CCAATCCGAA CACCCCGGCA CATCCGTCAG CATCCAACAG TCCCACGGCG 251 GCTCCAGCAA ACAGGCATTA TCCGTAGCCA ACGGCCTTCA AGCCGATGTC 301 GTAACCATGA ACCAATCCTC CGACATCGAC CTGCTCGAAA AAAAAGGACT 351 GGTAGAAAAA GGCTGGCAAC AAGCCCTCCC CGACCACGCC GCGCCCTACA 401 CCAGCACTAT GGTTTTCCTT GTCCGAAAAA ACAACCCCAA ACAGATCCGC 451 GATTGGAACG ACCTTGCCAA AGACGGCGTT AACATCGTCA TCGCCAATCC 501 CAAAACCTCG GGCAACGGAC GCTACGCCTT CCTCGGCGCA TACGGTTACG 551 GTCTGAAAAC CACCAACGGC AACGAACAGC AAGCCCAAAA ACTCGTCGCA 601 TCCATCCTCA AAAACACCCC CGTTTTTGAA AACGGCGGAC GCGCGCCACC 651 ACCACCTTCA CACAACGCAA CATCGGCGAC GTACTCATCA CTTTTGAAAA 701 CGAAGCCAAC TACGTCAGCA AAAAACTGA This corresponds to the amino acid sequence <SEQ ID 2844; ORF 927. a>: a927.pep 1 MKTYAPALYT AALLSACSPA ADSNHPSGQN APANTESDGK NITLLNASYD 51 VARDFYKEYN PLFIKTYQSE HPGTSVSIQQ SHGGSSKQAL SVANGLQADV 101 VTMNQSSDID LLEKKGLVEK GWQQALPDHA APYTSTMVFL VRKNNPKQIR 151 DWNDLAKDGV NIVIANPKTS GNGRYAFLGA YGYGLKTTNG NEQEAQKLVA 201 SILKNTPVFE NGGRAPPPPS HNATSATYSS LLKTKPTTSA KN* m927/a927 99.2% identity in 242 aa overlap 10 20 30 40 50 60 m927. pep MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITLLNASYDVARDFYKEYN MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a927 MKTYAPALYTAALLSACSPAADSNHPSGQNAPANTESDGKNITLLNASYDVARDFYKEYN 10 20 30 40 50 60 70 80 90 100 110 120 m927.pep PLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVTMNQSSDIDLLEKKGLVEK I II II I II I I II II II I II I I I II I II I I I I I I I II I II II II II II M II II I I I M I I a927 PLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVTMNQSSDIDLLEKKGLVEK 70 80 90 100 110 120 130 140 150 160 170 180 m927. pep GWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGVNIVIANPKTSGNGRYAFLGA I II I I I I I I II I M I II II II I I II I II M I II II II II I II [ II II I I I I II I II II I r a927 GWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGVNIVIANPKTSGNGRYAFLGA 130 140 150 160 170 180 190 200 210 220 230 240 m927. pep YGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRXPPPPSHNATSATYΞSLLKTKPTTSA I II I II II I II II II II I I I II I II II I II I M I II II I I II I I M I I II I II I II II I a927 YGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRAPPPPSHNATSATYSSLLKTKPTTSA 190 200 210 220 230 240 m927 . pep KNX I I I a927 KNX The following partial DNA sequence was identified in N.gonorrhoeae <SEQ ID 2845>: g929. seq 1 ATGAAATTGG GTTTCAAACC GATACCCCTC GCCATTGCCG CAGTATTGTG 51 CGCCCTGGTT TTGGCACTGC CCGTACccga CGGGGTCAAG CCTCAGGCTT 101 GGACGCTGCT GGCTATGTTT GTCGGTGTGA TTGCCGCCAT TATCGGCAAG 151 GTTATGCCGT TGGGCGCGCT GTCGATTATT GCCGTCGGGT TGGTCGCAGT 201 AACCGGCGTA ACCGCCGACA AACCGGGCGC GGCGATGAGC GATGCGTTGA 251 GTGCGTTCGC CAATCCGTTG ATTTGGCTGA TTGCCATCGC AGTTATGATT 301 TCGCGCGGTT TGCTCAAAAC AGGGCTGGGG ATGCGTATCG GATATTTGTT 351 TATCGCCGTT TTTGGAAGAA AAAcgctggG CATCGGTTAC AGTCTCGCTC 401 TTTCCGAACT GCTGCTGGCT CCCGTTACCC CTTCCAATAC CGCGCGCGGC 451 GGCGGCATTA TACATCcgaT TATGCagtcg attgCcggCA GttacggctC 501 caatCCCGCA AAAGGCACag aaggcaagat gggtaAATAT TtggcTTtgg 551 tcaattaTCA TTCcaaTCCC atttcgtcgg ctAtggctat taCTGcaact 601 gCCCCcaaCC CTTTAATcgt caacttgatt gccGaaaaTt taggcagtag 651 tttccgtCTT TCttgggggg cgTGGGcgtg ggcaaTGGCT Gttcccggcg 701 ttatcgcctt TTtcgTTATG CCTTTGATTT TATATTTTTT GTATCCGCCT 751 GAAATTAAAG AAACGCCCAA TGCTGttcAA TTTGCCAAAG ACCGTCTGAG 801 CGAGATGGGT AAAATGtcgg CAGACGAAAT CATTATGGCG GTCATTTTCG 851 GTATCTTGCT GCTGTTGTGG GCAGATGTTC CCGCCCTTAT TACCGGCAAT 901 CACGCTTTTA GTATCAacgc caccGCCACC GCATTTATCG GATTAAGCCT 951 GCTTTTGCTT TCCGGTGTAT TGACTTGGGA CGATGTTTTG AAAGAAAAAA 1001 GCGCGTGGGA TACGATTATT TGGTTTGGCG CATTGATTAT GATGGCCGCA 1051 TTTTTaAATA AActcggact gattaaatGG TTCTCCGGAG TGTTGGCGGA 1101 AagtgtcggC GGTTTGGGCG TTAGCGGCAC GGCTGCGGGC GTAATCCTCG 1151 TGCTTGCtta TATGTATGCG CATTATATGT TTGCCAGTAC TACTGCACAT 1201 ATTACCGCTA TGTTCGGCGC ATTTCTCGCT GCTGCCGTTT CACTGAATGC 1251 CCCGGCGATG CCGACTGCGC TGATGATGGC GGCCGCATCC AACATTATGA 1301 TGACCCTCAC TCATTATGCG ACCGGTACTT CACCTGTGAT TTTCGGCTCG 1351 GGCTACACCA CAATGGGAGA ATGGTGGAAG GCGGGTTTTA TCATGAGCGT 1401 AGTCAATTTT CTGATTTTTT CCGTTATCGG CAGCATTTGG TGGAAAGTTC 1451 TGGGATATTG GTAA This corresponds to the amino acid sequence <SEQ ID 2846; ORF 929.ng>: g929.pep 1 MKLGFKPIPL AIAAVLCALV LALPVPDGVK PQAWTLLAMF VGVIAAIIGK 51 VMPLGALSII AVGLVAVTGV TADKPGAAMS DALSAFANPL IWLIAIAVMI 101 SRGLLKTGLG MRIGYLFIAV FGRKTLGIGY SLALSELLLA PVTPSNTARG 151 GGIIHPIMQS IAGSYGSNPA KGTEGKMGKY LALVNYHSNP ISSAMAITAT 201 APNPLIVNLI AENLGSSFRL SWGAWAWAMA VPGVIAFFVM PLILYFLYPP 251 EIKETPNAVQ FAKDRLSEMG KMSADEIIMA VIFGILLLLW ADVPALITGN 301 HAFSINATAT AFIGLSLLLL SGVLTWDDVL KEKSAWDTII WFGALIMMAA 351 FLNKLGLIKW FSGVLAESVG GLGVSGTAAG VILVLAYMYA HYMFAΞTTAH 401 ITAMFGAFLA AAVSLNAPAM PTALMMAAAS NIMMTLTHYA TGTSPVIFGS 451 GYTTMGEWWK AGFIMSWNF LIFSVIGSIW WKVLGYW* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2847>: m929.seq 1 ATGAAATTGG GTTTCAAACC GATACCCCTC GCCATTGCCG CAGTATTGTG 51 CGCCCTGGTT TTGGCACTGC CCGTACCCGA CGGGGTCAAG CCTCAGGCTT 101 GGACGCTGCT GGCCATGTTT GTCGGTGTGA TTGCCGCCAT TATCGGCAAG 151 GCCATGCCGT TGGGCGCGCT GTCGATTATT GCCGTCGGGT TGGTCGCAGT 201 AACCGGCGTA ACCGCCGACA AACCGGGCGC GGCGATGAGC GATGCGTTGA 251 GTGCGTTCGC CAATCCGTTG ATTTGGCTGA TTGCCATCGC AGTTATGATT 301 TCGCGCGGTT TGCTCAAAAC AGGGCTGGGG ATGCGTATCG GATATTTGTT 351 TATCGCCGTT TTTGGAAGAA AAACGCTGGG CATCGGTTAC AGTCTCGCTC 401 TTTCCGAACT GCTGCTGGCT CCCGTTACCC CTTCCAATAC CGCGCGCGGC 451 GGCGGCATTA TACATCCGAT TATGCAGTCG ATTGCCGGCA GTTACGGCTC 501 CAATCCCGCA AAAGGCACAG AAGGCAAGAT GGGTAAATAT TTGGCTTTGG 551 TCAACTATCA TTCCAATCCC ATTTCGTCGG CTATGTTTAT TACTGCAACT 601 GCCCCCAACC CTTTAATCGT CAACTTGATT GCCGAAAATT TAGGCAGTAG 651 TTTCCGTCTT TCTTGGGGGG CGTGGGCGTG GGCAATGGCT GTTCCCGGCG 701 TTATCGCCTT TTTCGTTATG CCTTTGATTT TATATTTwyT GTATCCGCCT 751 GAAATTAAAG AAACGCCCAA TGCCGTTCAA TTTGCCAAAG ACCGTCTGAG 801 GGAGATGGGT AAAATGTCGG CAGACGAAAT CATTATGGCG GTCATTTTCG 851 GTATCTTGCT GCTGTTGTGG GCAGATGTTC CCGCCCTTAT TACCGGCAAT 901 CACGCTTTTA GTATCAACGC CACCGCCACC GCATTTATCG GATTAAGCCT 951 GCTTTTGCTT TCCGGTGTAT TGACTTGGGA CGATGTTTTG AAAGAAAAAA 1001 GCGCGTGGGA TACGATTATT TGGTTTGGCG CATTGATTAT GATGGCCGCA 1051 TTTTTAAATA AACTCGGACT GATTAAATGG TTCTCCGGAG TGTTGGCGGA 1101 AAGTGTCGGC GGTTTGGGCG TTAGCGGCAC GGCTGCGGGC GTAATCCTCG 1151 TGCTTGCTTA TATGTATGCG CATTATATGT TTGCCAGTAC TACTGCACAT 1201 ATTACCGCTA TGTTCGGCGC ATTTTTCGCT GCTGCCGTTT CACTGAATGC 1251 CCCGGCGATG CCGACCGCGC TGATGATGGC GgCCGCATCC AACATTATGA 1301 TGACCCTCAC TCATTATGCG ACCGGTACTT CGCCTGTGAT TTTCGGTTCG 1351 GGCTACACCA CAATGGGAGA ATGGTGGAAG GCGGGTTTTA TCATGAGCGT 1401 AGTCAATTTT CTGATTTTTT TCGTTATCGG CAGCATTTGG TGGAAAGTTC 1451 TGGGGTATTG GTAA This corresponds to the amino acid sequence <SEQ ID 2848; ORF 929>: m929.pep 1 MKLGFKPIPL AIAAVLCALV LALPVPDGVK PQAWTLLAMF VGVIAAIIGK 51 AMPLGALSII AVGLVAVTGV TADKPGAAMS DALSAFANPL IWLIAIAVMI 101 SRGLLKTGLG MRIGYLFIAV FGRKTLGIGY SLALSELLLA PVTPSNTARG 151 GGIIHPIMQS IAGSYGSNPA KGTEGKMGKY LALVNYHSNP ISSAMFITAT 201 APNPLIVNLI AENLGSSFRL SWGAWAWAMA VPGVIAFFVM PLILYXLYPP 251 EIKETPNAVQ FAKDRLREMG KMSADEIIMA VIFGILLLLW ADVPALITGN 301 HAFSINATAT AFIGLSLLLL SGVLTWDDVL KEKSAWDTII WFGALIMMAA 351 FLNKLGLIKW FSGVLAESVG GLGVSGTAAG VILVLAYMYA HYMFASTTAH 401 ITAMFGAFFA AAVSLNAPAM PTALMMAAAS NIMMTLTHYA TGTSPVIFGS 451 GYTTMGEWWK AGFIMSWNF LIFFVIGSIW WKVLGYW* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 929 shows 98.8% identity over a 487 aa overlap with a predicted ORF (ORF 929.ng) from N gonorrhoeae: g929/m929 10 20 30 40 50 60 g929.pep MKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGVIAAIIGKVMPLGALSII II II 1111111 M 11 h I M I M 111 m929 MKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGVIAAIIGKAMPLGALSII 10 20 30 40 50 60 70 80 90 100 110 120 g929.pep AVGLVAVTGVTADKPGAAMSDALSAFAΝPLIWLIAIAVMISRGLLKTGLGMRIGYLFIAV I j 11 I 111111111111 ! M 111111111 j 11 E 11 M I M 11 i i 11 M 111111111111 m929 AVGLVAVTGVTADKPGAAMSDALSAFAΝPLIWLIAIAVMISRGLLKTGLGMRIGYLFIAV 70 80 90 100 110 120 130 140 150 160 170 180 g929.pep FGRKTLGIGYSLALSELLLAPVTPSΝTARGGGIIHPIMQSIAGSYGSΝPAKGTEGKMGKY I ! 1111111111111111111 i 111 ! I J 11111 j 111 j 11111111111 F 1111111 I M m929 FGRKTLGIGYSLALSELLLAPVTPSΝTARGGGIIHPIMQSIAGSYGSΝPAKGTEGKMGKY 130 140 150 160 170 180 190 200 210 220 230 240 g929.pep LALVΝYHSΝPISSAMAITATAPΝPLIVΝLIAEΝLGSSFRLSWGAWAWAMAVPGVIAFFVM I I 11 I 11111111 I i I M j I M 11111111 II I E I M I M 11 M 111111 M 1111 I II m929 LALVΝYHSΝPISSAMFITATAPΝPLIVΝLIAEΝLGSSFRLSWGAWAWAMAVPGVIAFFVM 190 200 210 220 230 240 250 260 270 280 290 300 g929.pep PLILYFLYPPEIKETPNAVQFAKDRLSEMGKMSADEIIMAVIFGILLLLWADVPALITGN Mill m929 PLILYXLYPPEIKETPNAVQFAKDRLREMGKMSADEIIMAVIFGILLLLWADVPALITGN 250 260 270 280 290 300 310 320 330 340 350 360 g929 pep HAFSINATATAFIGLSLLLLSGVLTWDDVLKEKSAWDTIIWFGALIMMAAFLNKLGLIKW M M M I M M M M M I M M M M M M M M M M M M M M M M M ! M I I I M m929 HAFSINATATAFIGLSLLLLSGVLTWDDVLKEKSAWDTIIWFGALIMMAAFLNKLGLIKW 310 320 330 340 350 360 370 380 390 400 410 420 g929. pep FSGVLAESVGGLGVSGTAAGVILVLAYMYAHYMFASTTAHITAMFGAFLAAAVSLNAPAM I 1111111111 i M 1111111111111111111111111 ! M I 111 M H I 11 I 11111 I m929 FSGVLAESVGGLGVSGTAAGVILVLAYMYAHYMFASTTAHITAMFGAFFAAAVSLNAPAM 370 380 390 400 410 420 430 440 450 460 470 480 g929.pep PTALMMAAASNIMMTLTHYATGTSPVIFGSGYTTMGEWWKAGFIMSWNFLIFSVIGSIW m929 PTALMMAAASNIMMTLTHYATGTSPVIFGSGYTTMGEWWKAGFIMSWNFLIFFVIGSIW 430 440 450 460 470 480 g929.pep WKVLGYWX m929 WKVLGYWX The following partial DNA sequence was identified in N meningitidis <SEQ ID 2849>: a929.seq 1 ATGAAATTGG GTTTCAAACC GATACCCCTC GCCATTGCCG CAGTATTGTG 51 CGCCTTGGTT TTGGCACTGC CCGTACCCGA CGGGGTCAAG CCTCAGGCTT 101 GGACGCTGCT GGCCATGTTT ATCGGTGTGA TTGCCGCCAT TATCGGCAAG 151 GCCATGCCGT TGGGTGCGCT GTCGATTATT GCCGTCGGGT TGGTCGCAGT 201 AACCGGCGTA ACCGCCGACA AACCGGGTGC GGCGATGAGC GATGCGTTGA 251 GTGCGTTCGC CAATCCGTTG ATTTGGCTGA TTGCCATCGC AGTTATGATT 301 TCGCGCGGTT TGCTCAAAAC AGGGCTGGGG ATGCGTATCG GATATTTGTT 351 TATCGCCGTT TTTGGAAGAA AAACGCTGGG CATCGGTTAC AGTCTCGCTC 401 TTTCCGAACT GCTGCTGGCT CCCGTTACCC CTTCCAATAC CGCGCGCGGC 451 GGCGGCATTA TACATCCGAT TATGCAGTCG ATTGCCGGCA GTTACGGCTC 501 CAATCCCGCA AAAGGCACAG AAGGCAAGAT GGGTAAATAT TTGGCTTTGG 551 TCAACTATCA TTCCAATCCC ATTTCGTCGG CTATGTTTAT TACTGCAACT 601 GCCCCCAACC CTTTAATCGT CAACTTGATT GCCGAAAATT TAGGCAGTAG 651 TTTCCGTCTT TCTTGGGGGG CGTGGGCGTG GGCAATGGCT GTTCCCGGCG 701 TTATCGCCTT TTTCGTTATG CCTTTGATTT TATATTTTTT GTATCCGCCT 751 GAAATTAAAG AAACGCCCAA TGCCGTTCAA TTTGCCAAAG ACCGTCTGAG 801 GGAGATGGGT AAAATGTCGG CAGACGAAAT CATTATGGCG GTCATTTTCG 851 GTATCTTGTT GCTGTTGTGG GCAGATGTTC CCGCCCTTAT TACCGGCAAT 901 CACGCTTTTA GTATCAACGC CACCGCCACC GCATTTATCG GATTAAGCCT 951 GCTTTTGCTT TCCGGTGTAT TGACTTGGGA CGATGTTTTG AAAGAAAAAA 1001 GCGCGTGGGA TACGATTATT TGGTTTGGCG CATTGATTAT GATGGCCGCA 1051 TTTTTAAATA AACTCGGACT GATTAAATGG TTCTCCGGAG TGTTGGCGGA 1101 AAGTGTCGGC GGTTTGGGCG TTAGCGGCAC GGCTGCGGGC GTAATCCTCG 1151 TGCTTGCTTA TATGTATGCG CATTATATGT TTGCCAGTAC TACTGCACAT 1201 ATTACCGCTA TGTTCGGCGC ATTTTTCGCT GCTGCCGTTT CACTGAATGC 1251 CCCGGCGATG CCGACCGCGC TGATGATGGC GGCCGCATCT AACATTATGA 1301 TGACCCTCAC TCATTATGCG ACCGGTACTT CGCCTGTGAT TTTCGGTTCG 1351 GGCTACACCA CAATGGGAGA ATGGTGGAAG GCGGGTTTTA TCATGAGCGT 1401 AGTCAATTTT CTGATTTTTT TCGTTATCGG CAGCATTTGG TGGAAAGTTC 1451 TGGGGTATTG GTA?, This corresponds to the amino acid sequence <SEQ ID 2850; ORF 929. a>: a929.pep 1 MKLGFKPIPL AIAAVLCALV LALPVPDGVK PQAWTLLAMF IGVIAAIIGK 51 AMPLGALSII AVGLVAVTGV TADKPGAAMS DALSAFANPL IWLIAIAVMI 101 SRGLLKTGLG MRIGYLFIAV FFGGRRKKTTLLGGIIGGYY SLALSELLLA PVTPSNTARG 151 GGIIHPIMQS IAGSYGSNPA KGTEGKMGKY LALVNYHSNP ISSAMFITAT 201 APNPLIVNLI AENLGSSFRL SWGAWAWAMA VPGVIAFFVM PLILYFLYPP 251 EIKETPNAVQ FAKDRLREMG KMSADEIIMA VIFGILLLLW ADVPALITGN 301 HAFSINATAT AFIGLSLLLL SGVLTWDDVL KEKSAWDTII WFGALIMMAA 351 FLNKLGLIKW FSGVLAESVG GLGVSGTAAG VILVLAYMYA HYMFASTTAH 401 ITAMFGAFFA AAVSLNAPAM PTALMMAAAS NIMMTLTHYA TGTSPVIFGS 451 GYTTMGEWWK AGFIMSVVNF LIFFVIGSIW WKVLGYW* m929/a929 99.6% identity in 487 aa overlap 10 20 30 40 50 60 m929.pep MKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGVIAAIIGKAMPLGALSII I I II II I I II I II II II II II II I I II I II II I II II I II : I II II I I I I I I II I I I I II a929 MKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFIGVIAAIIGKAMPLGALSII 10 20 30 40 50 60 70 80 90 100 110 120 m929. pep AVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMISRGLLKTGLGMRIGYLFIAV MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a929 AVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMISRGLLKTGLGMRIGYLFIAV 70 80 90 100 110 120 130 140 150 160 170 180 m929.pep FGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQSIAGSYGSNPAKGTEGKMGKY MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a929 FGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQSIAGSYGSNPAKGTEGKMGKY 130 140 150 160 170 180 190 200 210 220 230 240 m929.pep LALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSSFRLSWGAWAWAMAVPGVIAFFVM I I II I II I I II I II II II II II I I I II II I II II II II I II II II I II II I I I II I I I II a929 LALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSSFRLSWGAWAWAMAVPGVIAFFVM 190 200 210 220 230 240 250 260 270 280 290 300 m929.pep PLILYXLYPPEIKETPNAVQFAKDRLREMGKMSADEIIMAVIFGILLLLWADVPALITGN Mill I I II II I II II II I I II II I I I I I II I I II II II I II I II I I I II I II I I I I II a929 PLILYFLYPPEIKETPNAVQFAKDRLREMGKMSADEIIMAVIFGILLLLWADVPALITGN 250 260 270 280 290 300 310 320 330 340 350 360 m929.pep HAFSINATATAFIGLSLLLLSGVLTWDDVLKEKSAWDTIIWFGALIMMAAFLNKLGLIKW II I II II I II II II I II I I I I I I II I II I I I I I I I I II I I II I I I I II I I II I II I I I I I a929 HAFSINATATAFIGLSLLLLSGVLTWDDVLKEKSAWDTIIWFGALIMMAAFLNKLGLIKW 310 320 330 340 350 360 370 380 390 400 410 420 m929. pep FSGVLAESVGGLGVSGTAAGVILVLAYMYAHYMFASTTAHITAMFGAFFAAAVSLNAPAM II II II I II II II II II I II II I I II II II II II II II I I I II II I II II II II II I II I a929 FSGVLAESVGGLGVSGTAAGVILVLAYMYAHYMFASTTAHITAMFGAFFAAAVSLNAPAM 370 380 390 400 410 420 430 440 450 460 470 480 m929.pep PTALMMAAASNIMMTLTHYATGTSPVIFGSGYTTMGEWWKAGFIMSVVNFLIFFVIGSIW MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a929 PTALMMAAASNIMMTLTHYATGTSPVIFGSGYTTMGEWWKAGFIMSVVNFLIFFVIGSIW 430 440 450 460 470 480 m929.pep WKVLGYWX II II II I I a929 WKVLGYWX g930.seq not found yet g930.pep not found yet The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2851>: m930.seq 1 ATGAAACTTC CTTTATCCTA TTTGCCTAAT ATTCGCTTTT TGTCTTGGTG 51 CTGCTTATTG GCAGGTATCA TTGCTCCTGC TACTTTGTTG GCCTCCCCCA 101 ACCCTGCCGA AATCCGTATG CAGCAAGATA TTCAGCAACG CCAACGCGAA 151 GAGCAGTTGC GCCAAACCAT GCAGCCTGAA AGCGATGTGC GTTTGCATCA 201 AAAAAACACG GGGGAAACGG TTAATCAGTT GATGGGCGAT GACAGCAGCC 251 AACCGTGTTT TGCCATTAAC GAAtGGGTGT TGGAAGGCGA ACACCATGCT 301 CGGTTTCAGT TTGCCCTAAA ACGTGCCTTG CGCGAAACGG GTTTTCAGGC 351 TGGCAAGTGT CTGCATGCGG GCAACATTAA TCAAATCATG TCCTTAGCAC 401 AAAATGCTTT GATCGGCAGG GGATATACCA CGACCCGTAT CTTGGCTGCG 451 CCACAGGATT TGAATAgTGG aAGCTTCAAT TAA This corresponds to the amino acid sequence <SEQ ID 2852; ORF 930>: m930.pep 1 MKLPLSYLPN IRFLSWCCLL AGIIAPATLL ASPNPAEIRM QQDIQQRQRE 51 EQLRQTMQPE SDVRLHQKNT GETVNQLMGD DSSQPCFAIN EWVLEGEHHA 101 RFQFALKRAL RETGFQAGKC LHAGNINQIM SLAQNALIGR GYTTTRILAA 151 PQDLNSGSFN * The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 2853>: g930-l.seq (partial) 1 GGCAAGTGTC TGCATGCGGG CGACATTAAT CAAATCATGT CCTTAGCACA 51 AAATGCTTTG ATCGGCAGGG GATATACCAC GACCCGTATC TTGGCTGCGC 101 CACAGGATTT GAATAGTGGC AAGCTTCAAT TAACCCTGAT GCCGGGCTAT 151 CTGCGCTCCA TACGAATCGA TCGGTCCAAC GATGATCAAA CCCATGCAGG 201 ACGTATTGCA GCATTCCAAA ACAAATTTCC CACCCGCTCG AACGATCTGT 251 TGAATCTGCG TGATTTGGAA CAAGGACTGG AAAATCTCAA ATGTCTCCCG 301 ACTGCGGAAG CCGATCTCCA AATCGTTCCC GTAGAGAGAG AACCAAACCA 351 AAGTGATGTC GTGGTGCAAT GGCGGTAACG TCTGCTGCCC TACTGTGTGA 401 GTGTGGGGAT GGATAATTCG GGTAGTGAGG CGACAGGAAA ATACCAAGGA 451 AATATCACTT TCTCTGCCGA CAATCCTTTT GGACTGAGTG ATATGTTCTA 501 TGTAAATTAT GGACGTTCAA TTGGCGGTAC GCCCGATGAG GAAAATTTTG 551 ACGGCCATCG CAAAGAAGGC GGATCAAACA ATTACGCCGT ACATTATTCA 601 GCCCCTTTCG GTAAATGGAC ATGGGCATTC AATCACAATG GCTACCGTTA 651 CCATCAGGCG GTTTCCGGAT TATCGGAAGT CTATGACTAT AATGGAAAAA 701 GTTACAACAC TGATTTCGGC TTCAACCGCC TGTTGTATCG TGATGCCAAA 751 CGCAAAACCT ATCTCAGTGT AAAACTGTGG ACGAGGGAAA CAAAAAGTTA 801 CATTGATGAT GCCGAACTGA CTGTACAACG GCGTAAAACC ACAGGTTGGT 851 TGGCAGAACT TTCCCACAAA GGATATATCG GTCGCAGTAC GGCAGATTTT 901 AAGTTGAAAT ATAAACACGG CACCGGCATG AAAGATGCTC TGCGCGCGCC 951 TGAAGAAGCC TTTGGCGAAG GCACGTCACG TATGAAAATT TGGACGGCAT 1001 CGGCTGATGT AAATACTCCT TTTCAAATCG GTAAACAGCT ATTTGCCTAT 1051 GACACATCCG TTCATGCACA ATGGAACAAA ACCCCGCTAA CATCGCAAGA 1101 CAAACTGGCT ATCGGCGGAC ACCACACCGT ACGTGGCTTC GACGGTGAAA 1151 TGAGTTTGCC TGCCGAGCGG GGATGGTATT GGCGCAACGA TTTGAGCTGG 1201 CAATTTAAAC CAGGCCATCA GCTTTATCTT GGGGCTGATG TAGGACATGT 1251 TTCAGGACAA TCCGCCAAAT GGTTATCGGG CCAAACTCTA GCCGGCACAG 1301 CAATTGGGAT ACGCGGGCAG ATAAAGCTTG GCGGCAACCT GCATTACGAT 1351 ATATTTACCG GCCGTGCATT GAAAAAGCCC GAATATTTTC AGACGAAGAA 1401 ATGGGTAACG GGGTTTCAGG TGGGTTATTC GTTTTGA This corresponds to the amino acid sequence <SEQ ID 2854; ORF 930-1. ng>: g930-l.pep (partial) 1 GKCLHAGDIΝ QIMSLAQΝAL IGRGYTTTRI LAAPQDLΝSG KLQLTLMPGY 51 LRSIRIDRSΝ DDQTHAGRIA AFQΝKFPTRS ΝDLLΝLRDLE QGLEΝLKCLP 101 TAEADLQIVP VEREPΝQSDV VVQWR*RLLP YCVSVGMDΝS GSEATGKYQG 151 ΝITFSADΝPF GLSDMFYVΝY GRSIGGTPDE EΝFDGHRKEG GSΝΝYAVHYS 201 APFGKWTWAF ΝHΝGYRYHQA VSGLSEVYDY ΝGKSYΝTDFG FΝRLLYRDAK 251 RKTYLSVKLW TRETKSYIDD AELTVQRRKT TGWLAELSHK GYIGRSTADF 301 KLKYKHGTGM KDALRAPEEA FGEGTSRMKI WTASADVΝTP FQIGKQLFAY 351 DTSVHAQWΝK TPLTSQDKLA IGGHHTVRGF DGEMSLPAER GWYWRΝDLSW 401 QFKPGHQLYL GADVGHVSGQ SAKWLSGQTL AGTAIGIRGQ IKLGGΝLHYD 451 IFTGRALKKP EYFQTKKWVT GFQVGYSF* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2855>: 930-l.seq 1 ATGAAACTTC CTTTATCCTA TTTGCCTAAT ATTCGCTTTT TGTCTTGGTG 51 CTGCTTATTG GCAGGTATCA TTGCTCCTGC TACTTTGTTG GCCTCCCCCA 101 ACCCTGCCGA AATCCGTATG CAGCAAGATA TTCAGCAACG CCAACGCGAA 151 GAGCAGTTGC GCCAAACCAT GCAGCCTGAA AGCGATGTGC GTTTGCATCA 201 AAAAAACACG GGGGAAACGG TTAATCAGTT GATGGGCGAT GACAGCAGCC 251 AACCGTGTTT TGCCATTAAC GAAGTGGTGT TGGAAGGCGA ACACCATGCT 301 CGGTTTCAGT TTGCCCTAAA ACGTGCCTTG CGCGAAACGG GTTTTCAGGC 351 TGGCAAGTGT CTGCATGCGG GCAACATTAA TCAAATCATG TCCTTAGCAC 401 AAAATGCTTT GATCGGCAGG GGATATACCA CGACCCGTAT CTTGGCTGCG 451 CCACAGGATT TGAATAGTGG CAAGCTTCAA TTAACCCTGA TACCGAGCTA 501 TCTGCGCTCC ATACGAATCG ATCGGTCTAA CGATGATCAA ACCCATGCAG 551 GACGTATTGC AGCATTCCAG AACAAATTTC CCACCCGCTC GAACGATCTG 601 TTGAATCTGC GTGATTTGGA ACAAGGACTG GAAAATCTCA AACGTCTCCC 651 GACTGCGGAA GCCGATCTCC AAATCGTTCC CGTAGAGGGA GAACCAAACC 701 AAAGTGATGT CGTGGTGCAA TGGCGGCAAC GTCTGCTGCC CTACCGTGTG 751 AGTGTGGGGA TGGATAATTC GGGTAGTGAG GCGACAGGAA AATACCAAGG 801 AAATATCACT TTCTCTGCCG ACAATCCTTT GGGACTGAGT GATATGTTCT 851 ATGTAAATTA TGGACGTTCG ATTGGCGGTA CGCCCGATGA GGAAAGTTTT 901 GACGGCCATC GCAAAGAAGG CGGATCAAAC AATTACGCCG TACATTATTC 951 AGCCCCTTTC GGTAAATGGA CATGGGCATT CAATCACAAT GGCTACCGTT 1001 ACCATCAGGC AGTTTCCGGA TTATCGGAAG TCTATGACTA TAATGGAAAA 1051 AGTTACAATA CTGATTTCGG CTTCAACCGC CTGTTGTATC GTGATGCCAA 1101 ACGCAAAACC TATCTCGGTG TAAAACTGTG GATGAGGGAA ACAAAAAGTT 1151 ACATTGATGA TGCCGAACTG ACTGTACAAC GGCGTAAAAC TGCGGGTTGG 1201 TTGGCAGAAC TTTCCCACAA AGAATATATC GGTCGCAGTA CGGCAGATTT 1251 TAAGTTGAAA TATAAACGCG GCACCGGCAT GAAAGATGCT CTGCGCGCGC 1301 CTGAAGAAGC CTTTGGCGAA GGCACGTCAC GTATGAAAAT TTGGACGGCA 1351 TCGGCTGATG TAAATACTCC TTTTCAAATC GGTAAACAGC TATTTGCCTA 1401 TGACACATCC GTTCATGCAC AATGGAACAA AACCCCGCTA ACATCGCAAG 1451 ACAAACTGGC TATCGGCGGA CACCACACCG TACGTGGCTT CGACGGTGAA 1501 ATGAGTTTGT CTGCCGAGCG GGGATGGTAT TGGCGCAACG ATTTGAGCTG 1551 GCAATTTAAA CCAGGCCATC AGCTTTATCT TGGGGCTGAT GTAGGACATG 1601 TTTCAGGACA ATCCGCCAAA TGGTTATCGG GCCAAACTCT AGTCGGCACA 1651 GCAATTGGGA TACGCGGGCA GATAAAGCTT GGCGGCAACC TGCATTACGA 1701 TATATTTACC GGCCGCGCAT TGAAAAAGCC CGAATTTTTC CAATCAAGGA 1751 AATGGGCAAG CGGTTTTCAG GTAGGCTATA CGTTTTAA This corresponds to the amino acid sequence <SEQ ID 2856; ORF 930- 1>: m930-l.pep 1 MKLPLSYLPΝ IRFLSWCCLL AGIIAPATLL ASPΝPAEIRM QQDIQQRQRE 51 EQLRQTMQPE SDVRLHQKΝT GETVΝQLMGD DSSQPCFAIΝ EVVLEGEHHA 101 RFQFALKRAL RETGFQAGKC LHAGΝIΝQIM SLAQΝALIGR GYTTTRILAA 151 PQDLΝSGKLQ LTLIPSYLRS IRIDRSΝDDQ THAGRIAAFQ ΝKFPTRSΝDL 201 LΝLRDLEQGL EΝLKRLPTAE ADLQIVPVEG EPΝQSDVVVQ WRQRLLPYRV 251 SVGMDΝSGSE ATGKYQGΝIT FSADΝPLGLS D FYVΝYGRS IGGTPDEESF 301 DGHRKEGGSΝ ΝYAVHYSAPF GKWTWAFΝHΝ GYRYHQAVSG LSEVYDYΝGK 351 SYΝTDFGFΝR LLYRDAKRKT YLGVKLWMRE TKSYIDDAEL TVQRRKTAGW 401 LAELΞHKEYI GRSTADFKLK YKRGTGMKDA LRAPEEAFGE GTSRMKIWTA 451 SADVΝTPFQI GKQLFAYDTS VHAQWΝKTPL TSQDKLAIGG HHTVRGFDGE 501 MSLSAERGWY WRΝDLSWQFK PGHQLYLGAD VGHVSGQSAK WLSGQTLVGT 551 AIGIRGQIKL GGΝLHYDIFT GRALKKPEFF QSRKWASGFQ VGYTF* m930-l/g930-l 95.4% identity in 478 aa overlap 90 100 110 120 130 140 m930-l.pep AIΝEVVLEGEHHARFQFALKRALRETGFQAGKCLHAGΝIΝQIMSLAQΝALIGRGYTTTRI I I I I I I M I I I I I II I I I I II I I I I I I I I I g930-l .pep GKCLHAGDIΝQIMΞLAQΝALIGRGYTTTRI 10 20 30 150 160 170 180 190 200 m930-l.pep LAAPQDLΝSGKLQLTLIPΞYLRSIRIDRSΝDDQTHAGRIAAFQΝKFPTRSΝDLLΝLRDLE I I II I I I I I II I II I I : M II I I I I I I I I I I I I I I I I I II I II I I I I I I I I I I I I II I II g930-l.pep LAAPQDLΝSGKLQLTLMPGYLRSIRIDRSΝDDQTHAGRIAAFQΝKFPTRSΝDLLΝLRDLE 40 50 60 70 80 90 210 220 230 240 250 260 m930-l . pep Q GLEΝLKRLPTAEADLQIVPVEGEPΝ Q SDVVVQWRQRLLPYRVSVGMDΝSGSEATGKYQG I I I I I I I I I I I II I 11 I I I I I I I II I I I I I I I I I I I I I I I I II I II I II g930-l.pep QGLENLKCLPTAEADLQIVPVEREPNQSDVVVQWRXRLLPYCVSVGMDNSGSEATGKYQG 100 110 120 130 140 150 270 280 290 300 310 320 m930-l.pep NITFSADNPLGLSDMFYVNYGRΞIGGTPDEESFDGHRKEGGSNNYAVHYSAPFGKWTWAF I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I g930-l.pep NITFSADNPFGLSDMFYVNYGRΞIGGTPDEENFDGHRKEGGSNNYAVHYSAPFGKWTWAF 160 170 180 190 200 210 330 340 350 360 370 380 m930-l.pep NHNGYRYHQAVSGLSEVYDYNGKSYNTDFGFNRLLYRDAKRKTYLGVKLWMRETKSYIDD I I I I II I I I I I I I I I I I I I II II I I I I I I I I I I I I I I II I I I I I I : I II I I I I I I I I II g930-l.pep NHNGYRYHQAVSGLSEVYDYNGKSYNTDFGFNRLLYRDAKRKTYLSVKLWTRETKSYIDD 220 230 240 250 260 270 390 400 410 420 430 440 m930-l.pep AELTVQRRKTAGWLAELSHKEYIGRSTADFKLKYKRGTGMKDALRAPEEAFGEGTSRMKI I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I M I I I I I I I I I I I I I I I I I I I I I I I I g930-l.pep AELTVQRRKTTGWLAELSHKGYIGRSTADFKLKYKHGTGMKDALRAPEEAFGEGTSRMKI 280 290 300 310 320 330 450 460 470 480 490 500 Itl930-l.pep WTASADVNTPFQIGKQLFAYDTSVHAQWNKTPLTΞQDKLAIGGHHTVRGFDGEMSLSAER I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g930-l.pep WTASADVNTPFQIGKQLFAYDTSVHAQWNKTPLTSQDKLAIGGHHTVRGFDGEMSLPAER 340 350 360 370 380 390 510 520 530 540 550 560 m930-l.pep GWYWRNDLSWQFKPGHQLYLGADVGHVΞGQSAKWLSGQTLVGTAIGIRGQIKLGGNLHYD I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I g930-l.pep GWYWRNDLSWQFKPGHQLYLGADVGHVSGQΞAKWLSGQTLAGTAIGIRGQIKLGGNLHYD 400 410 420 430 440 450 570 580 590 m930-l .pep IFTGRALKKPEFFQSRKWASGFQVGYTF I I I I I I I I I I I M M: I MM I I I I M I g930-1.pep IFTGRALKKPE FQTKKWVTGFQVGYSFX 460 470 a930-l.seq not yet found a930-l.pep not yet found The following partial DNA sequence was identified in N.gonorrhoeae <SEQ ID 2857>: g931.seq 1 ATGAAACCCA AATTCAAAAC CGTTTTAACC GCGCTGCTTT TGGCGGTTTC 51 CCTGCCGTCT ATGGCGGCAA CCCGCGTCCT GATGGAAACC GATATGGGCA 101 ATATCCGTTT GGTTTTGGAC GAATCCAAAG CCTCCAAAAC CGTTGCCAAT 151 TTCGTGCGCT ATGCCCGAAA AGGCTTTTAC GACAACACGA TTTTCCACCG 201 CGTcatCGGC GGCTTCGTCA TCCAAGGCGA CGGATTGACC GAGGACTTGG 251 TGCAAAAGGC AACCGATAAG GCCGTTGCCA ACGAATCCGG caacgGCTTG 301 AAAAACACCG TCGGCACCAT CGCAATGGCG CGGACGGCAG CCCCCGATTC 351 CGCCGCCGCC CAATTCTTTA TCAATCTGGC GGACAACGGT TCGCTCGACT 401 ACAAAAACGG ACAATACGGC TACACCGTTT TCGGCAGGGT AGAAAGCGGA 451 ATGGACACCG TTTCCAAAAT CGCCCGCGTC AAAACCGCCA CGCGCGGCTT 501 TTATCAAAAC GTACCCGTAC AGCCCGTCAA AATCCGTCGC GTTGTTGTCG 551 GGCAGTAACA CGCAGACAGA CGTTCAGACG GCGTCGCCCG TTTCCCAAAA 601 AACGCCGTTT AA This corresponds to the amino acid sequence <SEQ ID 2858; ORF 931.ng>: g931.pep 1 MKPKFKTVLT ALLLAVSLPS MAATRVLMET DMGNIRLVLD ESKASKTVAN 51 FVRYARKGFY DNTIFHRVIG GFVIQGDGLT EDLVQKATDK AVANESGNGL 101 KNTVGTIAMA RTAAPDSAAA QFFINLADNG SLDYKNGQYG YTVFGRVESG 151 MDTVSKIARV KTATRGFYQN VPVQPVKIRR VGQ* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2859>: m931. seq 1 ATGAAACCCA AATTCAAAAC CGTTTTAACC GCGCTGCTTT TGGCGGTTTC 51 CCTGCCGTCT ATGGCGGCAA CCCATGTTTT GATGGAAACC GATATGGGCA 101 ATATCCGTTT GGTTTTGGAC GAATCCAAAG CCCCCAAAAC CGTTGCTAAT 151 TTCGTGCGCT ATGCCCGAAA AGGCTTTTAC GACGACACCG TTTTTCACCG 201 CGTTATCGAC GGTTTTGTTA TCCAGGGCGG TGGATTGACC GAGGACTTGG 251 CACAAAAGGC AAGCGATAAG GCCGTTGCCA ACGAATCCGG CAACGGCTTG 301 AAAAACACCG CCGGCACCAT CGCCATGGCG CGGACGACAG CCCCCGATTC 351 CGCCACCAGC CAATTCTTTA TCAATCTGGC GGACcA.kCT TCGCTCGACT 401 ACAAAAACGG ACAATACGGC TATACCGTTT TCGGCAGGGT CGAAAGCGGC 451 ATGAACACCG TTTCCAAAAT CGCCCGCGTC AAAACCGCCA CGCGCGGCTT 501 TTATCAAAAC GTACCCGTAC AGCCCGTCAA AATCCGTCGC GTTGTTGTCG 551 GGCAGTAA This corresponds to the amino acid sequence <SEQ ID 2860; ORF 931>: m931.pep.. 1 MKPKFKTVLT ALLLAVSLPS MAATHVLMET DMGNIRLVLD ESKAPKTVAN 51 FVRYARKGFY DDTVFHRVID GFVIQGGGLT EDLAQKASDK AVANESGNGL 101 KNTAGTIAMA RTTAPDSATS QFFINLADXX SLDYKNGQYG YTVFGRVESG 151 MNTVSKIARV KTATRGFYQN VPVQPVKIRR VWGQ* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 931 shows 91.9% identity over a 185 aa overlap with a predicted ORF (ORF 93 l.ng) from N gonorrhoeae: g93l/m931 10 20 30 40 50 60 g931.pep MKPKFKTVLTALLLAVSLPSMAATRVLMETDMGΝIRLVLDESKASKTVAΝFVRYARKGFY M M M M M M I M m931 MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGΝIRLVLDESKAPKTVAΝFVRYARKGFY 10 20 30 40 50 60 70 80 90 100 110 120 g931. pep DΝTIFHRVIGGFVIQGDGLTEDLVQKATDKAVAΝESGΝGLKΝTVGTIAMARTAAPDSAAA h h l l l M 1 1 1 1 M : 1 1 h 1 1 1 1 1 I I 1 1 1 I I 1 1 1 : 1 1 1 1 1 I I h I I 1 1 1 = = m931 DDTVFHRVIDGFVIQGGGLTEDLAQKASDKAVAΝESGΝGLKΝTAGTIAMARTTAPDSATS 70 - 80 90 100 110 120 130 140 150 160 170 180 g931. pep QFFIΝLADΝGSLDYKΝGQYGYTVFGRVESGMDTVSKIARVKTATRGFYQΝVPVQPVKIRR m931 QFFIΝLADXXSLDYKΝGQYGYTVFGRVESGMΝTVSKIARVKTATRGFYQΝVPVQPVKIRR 130 140 150 160 170 180 g931 . pep VWGQX M M M m931 VWGQX The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2861 >: a931. seq 1 ATGAAACCCA AATTCAAAAC CGTTTTAACC GCGCTGCTTT TGGCGGTTTC 51 CCTGCCGTCT ATGGCGGCAA CCCATGTTTT GATGGAAACC GATATGGGCA 101 ATATCCGTTT GGTTTTGGAC GAATCCAAAG CACCCAAAAC CGTTGCCAAT 151 TTCGTGCGCT ATGCCCGAAA AGGCTTTTAC GACAATACGA TTTTTCACCG 201 CGTCATCGGC GGCTTCGTTA TCCAAGGCGG CGGATTGACC GAGGACTTGG 251 CACAAAAGGC AAGCGATAAG GCCGTTGCCA ACGAATCCGG CAACGGCTTG 301 AAAAACACTG TCGGCACCAT CGCCATGGCG CGGACGGCCG ATCCGGATTC 351 CGCCACCAGC CAATTCTTTA TCAATCTGGT GGACAATGAT TCGCTCAACT 401 ACAAAAACGG ACAATACGGC TATACCGTTT TCGGCAGGGT CGAAAGCGGC 451 ATGAACACCG TTTCCAAAAT CGCCCGCGTC AAAACCGCCA CGCGCGGCTT 501 TTATCAAAAC GTACCCGTAC AGCCCGTCAA AATCCGTCGC GTTGTTGTCG 551 GGCAGTAA This corresponds to the amino acid sequence <SEQ ID 2862; ORF 931.a>: a931.pep 1 MKPKFKTVLT ALLLAVSLPS MAATHVLMET DMGNIRLVLD ESKAPKTVAN 51 FVRYARKGFY DNTIFHRVIG GFVIQGGGLT EDLAQKASDK AVANESGNGL 101 KNTVGTIAMA RTADPDSATS QFFINLVDND SLNYKNGQYG YTVFGRVESG 151 MNTVSKIARV KTATRGFYQN VPVQPVKIRR VWGQ* m93 l/a931 94.6% identity in 185 aa overlap 10 20 30 40 50 60 m931. pep MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKAPKTVANFVRYARKGFY MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a931 MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKAPKTVANFVRYARKGFY 10 20 30 40 50 60 70 80 90 100 110 120 m931.pep DDTVFHRVIDGFVIQGGGLTEDLAQKASDKAVANESGNGLKNTAGTIAMARTTAPDSATS M I : I II II II I II II II I II II I II I II I II II II I II I II : I I I I I I I M MMM a931 DNTIFHRVIGGFVIQGGGLTEDLAQKASDKAVANESGNGLKNTVGTIAMARTADPDSATS 70 80 90 100 110 120 130 140 150 160 170 180 m931.pep QFFINLADXXSLDYKNGQYGYTVFGRVESGMNTVSKIARVKTATRGFYQNVPVQPVKIRR IIIIIMI I M II I I I I I II I I II I II II I I II I I I I I II II II II I I I II I I II I II a931 QFFINLVDNDSLNYKNGQYGYTVFGRVESGMNTVSKIARVKTATRGFYQNVPVQPVKIRR 130 140 150 160 170 180 m931.pep V GQX MMM a931 VWGQX g932.seq not found yet g932.pep not found yet The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2863>: m932. seq 1 ATGAAATATA TCGTATCAAT CTCTCTGGCT ATGGGATTGG CTGCCTGTTC 51 GTTTGGGGGA TTTAAACCAA ATCCGTGGGA CGCCGCGTCA TTTTGGGAAT 101 TGAAAAATTA CGCCAATCCC TATCCGGGAT CAGCCTCGGC GGCACTTGAC 151 CAATATCCAT CGAAAGCAAG ACGAAGGCAA CTGAAAGACA TGCAAGAGTG 201 CGGCTATGAC CCAATAGACG GCGGAAAGTC TGAAGCAGAT GCCTGCCTGA 251 GGAAAAAAGG CTGGTGTCGT AAGGGTTTCG ACCCTTATCC CGAAAACAAA 301 AAATACGAAT GGCCTCGAGA AGAAGGAAAA ACAAAATGA This corresponds to the amino acid sequence <SEQ ID 2864; ORF 932>: m932.pep 1 MKYIVSISLA MGLAACSFGG FKPNPWDAAS FWELKNYANP YPGSASAALD 51 QYPSKARRRQ LKDMQECGYD PIDGGKSEAD ACLRKKGWCR KGFDPYPENK 101 KYEWPREEGK TK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 932 shows % identity over a aa overlap with a predicted ORF (ORF 932.ng) from N gonorrhoeae: The following partial DΝA sequence was identified in N.gonorrhoeae <SEQ ID 2865>: g934. seq 1 ATGAAAAAAA TCATCGCCTC CGCGCTTATC GCAACATTCG CACTCACCGC 51 CTGCCAAGAC GACACGCAGG CGCGGCTCGA ACGGCAGCAG AAACAGATTG 101 AAGCCCTGCA ACAGCAGCTC GCACAGCAGG CAGACGATAC GGTTTACCAA 151 CTGACTCCCG AAGCAGTCAA AGACACCATT CCTGCCCAGG CGCAGGCAAA 201 CGGCAACAAC GGTCAGCCCG TTACCGGCAA .AGAcggGCA GCAGTATATT 251 TACGACCAAT CGACAGGAAG CTGGCTGCTG CAAAGCCTGA TTGGCGCGGC 301 GGCAGGCGCG TTTATCGGCA ACGCGCTGGC AAACAAATTC ACACGGGCGG 351 GCAACCAAGA CAGCCCCGTC GCCCGTCGCG CGCGTGCTGC CTACCATCAG 401 TCCGCACGCC CCAATGCGCG CACCAGCAGG GATTTGAACA CGCGCAGCCT 451 CCGTGCAAAA CAACAGGCGG CGCAGGCGCA GCGTTACCGC CCGACAACGC 501 GCCCGCCCGT CAAttaccgc catcgcgcta tgcGCGGTTT CGgcagAagg 551 cggtaaaCCC GGCGCGTCAA TGCCGTCTGA AGGGCTTTCA GACGGCATTT 601 TTGTATTTGT TAGGGGCATT GTTATGTTGC CGTTTGATTT TCAGACGGCA 651 TTTTGTTTCC AAGCGTTTGA TGTcggGATG GCAATTCTGA This corresponds to the amino acid sequence <SEQ ID 2866; ORF 934.ng>: g934.pep 1 MKKIIASALI ATFALTACQD DTQARLERQQ KQIEALQQQL AQQADDTVYQ 51 LTPEAVKDTI PAQAQANGNN GQPVTGKRRA AVYLRPIDRK LAAAKPDWRG 101 GRRVYRQRAG KQIHTGGQPR QPRRPSRACC LPSVRTPQCA HQQGFEHAQP 151 PCKTTGGAGA ALPPDNAPAR QLPPSRYARF RQKAVNPARQ CRLKGFQTAF 201 LYLLGALLCC R IFRRHFVS KRLMSGWQF* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2867>: m934.seq (partial) 1 .. CGGCTCGAAC AGCAGCAGAA ACAGATTGAA GCCCTGCAAC AGCAGCTCGC 51 ACAGCAGGCA GACGATACGG TTTACCAACT GACTCCCGAA GCAGTCAAAG 101 ACACCATTCC TGCCGAAGCA CAGGCAAACG GCAACAACgG GCAACCCGTT 151 ACCGGTAA.A GACGGGCAGC AGTATATTTA CGACCAATCG ACAGGAAGCT 201 GGCTGCTGCA AAGCCTGGTC GGCGCGGCGG CAGGCGCGTT TATCGGCAAC 251 GCGCTGGCAA ACAAATTCAC ACGGGCAGGC AACCAAGACA GTCCCGTCGC 301 CCGGCGCGCG CGTGCAGCCT ACCATCAGTC CGCACGCCCC AATGCGCGCA 351 yCAGCAGGGA TTTGAACACG CGCAGCCTCC GTGCAAAACA ACAGGCGGCG 401 CAkGCGCAGC GTTACCGCCC GACAACGCGC CCGsCCGsCA ATTACCGCCG 451 CCCCGCTATG CGCGGTTTCG GCAGGAGGCG GTAAACCCGG CGCGCCAATG 501 CCGTCTGAAG AGCTTTCAGA CGGCATTTnT GCATTTGTTA GGGACATTGT 551 TATGTTGCCG TTTGATTTTC AGACGGCATT TTGTTTCCAA GCGTTTGATG 601 TCGGGATGGC AATTCTGA This corresponds to the amino acid sequence <SEQ ID 2868; ORF 934>: m934.pep (partial) 1 ..RLEQQQKQIE ALQQQLAQQA DDTVYQLTPE AVKDTIPAEA QANGNNGQPV 51 TGXRRAAVYL RPIDRKLAAA KPGRRGGRRV YRQRAGKQIH TGRQPRQSRR 101 PARACSLPSV RTPQCAHQQG FEHAQPPCKT TGGAXAALPP DNAPXRQLPP 151 PRYARFRQEA VNPARQCRLK SFQTAFXHLL GTLLCCRLIF RRHFVSKRLM 201 SGWQF* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 934 shows 91.7% identity over a 205 aa overlap with a predicted ORF (ORF 934.ng) from N gonorrhoeae: m934/g934 10 20 30 m934.pep RLEQQQKQIEALQQQLAQQADDTVYQLTPEAVKDTI M h l M M M M M M M M M M M M M I M M g934 MKKI IASALIATFALTACQDDTQARLERQQKQIEALQQQLAQQADDTVYQLTPEAVKDTI 10 20 30 40 50 60 40 50 60 70 80 90 m934 . pep PAEAQAΝGΝΝGQPVTGXRRAAVYLRPIDRKLAAAKPGRRGGRRVYRQRAGKQIHTGRQPR I h l M I I M I I I M I M M M M M I M M M M M M M I M M M I M M I I I g934 PA Q AQAΝGΝΝGQPVTGKRRAAVYLRPIDRKLAAAKPDWRGGRRVYRQRAGKQIHTGGQPR 70 80 90 100 110 120 100 110 120 130 140 150 m934 . pep QSRRPARACSLPSVRTPQCAHQQGFEHAQPPCKTTGGAXAALPPDNAPXRQLPPPRYARF I M h l l l M M M M M M M M M I M I M M M M i l l I M M g934 QPRRPSRACCLPSVRTPQCAHQQGFEHAQPPCKTTGGAGAALPPDNAPARQLPPSRYARF 130 140 150 160 170 180 160 170 180 190 200 m934.pep RQEAVNPARQCRLKSFQTAFXHLLGTLLCCRLIFRRHFVSKRLMSGWQFX I h l M M M M I h l l M I = I I I = I I I I I I I I I I I I I I I I M I I I I I I g934 RQKAVNPARQCRLKGFQTAFLYLLGALLCCRLIFRRHFVSKRLMSGWQFX 190 200 210 220 230 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2869>: a934. seq 1 ATGAAAAAAA TCATCGCCTC CGCGCTTATC GCAACATTCG CACTCGCCGC 51 CTGCCAAGAC GACGCGCAGG CGCGGCTCGA ACAGCAGCAG AAACAGATTG 101 AAGCCCTGCA ACAGCAGCTC GCACAGCAGG CAGACGATAC GGTTTACCAA 151 CTGACTCCCG AAGCAGTCAA AGACACCATT CCTGCCGAAG CACAGGCAAA 201 CGGCAACAAC GGGCAACCCG TTACCGG.TA AAGACGGGCA GCAGTATATT 251 TACGACCAAT CGACAGGAAG CTGGCTGCTG CAAAGCCTGG TCGGCGCGGC 301 GGCAGGCGCG TTTATCGGCA ACGCGCTGGC AAACAAATTC ACACGGGCAG 351 GCAACCAAGA CAGTCCCGTC GCCCGGCGCG CGCGTGCCGC CTACCATCAG 401 TCCGCACATC CCAATGCGCG CACCAGCAGG GATTTGAACA CGCGCAGCCT 451 CCGTGCAAAA CAACAGGCGG CGCAGGCGCA GCGTTACCGC CCGACAACGC 501 GCCCGCCCGC CAATTACCGC CGCCCCGCCA TGCGCGGTTT CGGCAGAAGG 551 CGGTAAATCC GGCGTGCCAA TGCCGTCTGA AGGGCTTTCA GACGGCATTT 601 TTGTATTTGT TAGGGACATT GTTATGTTGC CGTTTGATTT TTAGACGGCA 651 TTTTGTTTCC AAGAGTTTGA TGTCGGGATG GCAATTCTGA This corresponds to the amino acid sequence <SEQ ID 2870; ORF 934. a>: a934.pep 1 MKKIIASALI ATFALAACQD DAQARLEQQQ KQIEALQQQL AQQADDTVYQ 51 LTPEAVKDTI PAEAQAΝGΝΝ GQPVTX*RRA AVYLRPIDRK LAAAKPGRRG 101 GRRVYRQRAG KQIHTGRQPR QSRRPARACR LPSVRTSQCA HQQGFEHAQP 151 PCKTTGGAGA ALPPDΝAPAR QLPPPRHARF RQKAVΝPACQ CRLKGFQTAF 201 LYLLGTLLCC RLIFRRHFVS KSLMSGWQF* m934/a934 94.1 % identity in 205 aa overlap 10 20 30 m934. pep RLEQQQKQIEALQQQLAQQADDTVYQLTPEAVKDTI II II II II I I II I I I I I I I II I I I II I II II I II II a934 MKKIIASALIATFALAACQDDAQARLEQQQKQIEALQQQLAQQADDTVYQLTPEAVKDTI 10 20 30 40 50 60 40 50 60 70 80 90 m934. pep PAEAQAΝGΝΝGQPVTGXRRAAVYLRPIDRKLAAAKPGRRGGRRVYRQRAGKQIHTGRQPR I I I II I I II II I II I II II II II I I I I I I I II II I I II I I I I I II I I I II II I II II I I a934 PAEAQAΝGΝΝGQPVTXXRRAAVYLRPIDRKLAAAKPGRRGGRRVYRQRAGKQIHTGRQPR 70 80 90 100 110 120 100 110 120 130 140 150 m934. pep QSRRPARACSLPSVRTPQCAHQQGFEHAQPPCKTTGGAXAALPPDΝAPXRQLPPPRYARF II II I I I II MMM II II I II I I I I I II II II II I I I I I I II I I II I I I II : II I a934 QSRRPARACRLPSVRTSQCAHQQGFEHAQPPCKTTGGAGAALPPDΝAPARQLPPPRHARF 130 140 150 160 170 180 160 170 180 190 200 m934.pep RQEAVΝPARQCRLKSFQTAFXHLLGTLLCCRLIFRRHFVSKRLMSGWQFX I I : I I I II II I I I : II II I : I II I I I II I II II I I I II I llllllll a93 RQKAVΝPACQCRLKGFQTAFLYLLGTLLCCRLIFRRHFVSKSLMSGWQFX 190 200 210 220 230 g935.seq not found yet 1349 601 AATAGAAATG CCAATAATGC CGCGCCGCAG TATTGCCGGC AAAACGGAGG 651 CCGGCAGATA TGCAGTGTCA GCCGGGCGGA GCGGGCGGCA GGCTTGAATT 701 ATGAAATCGA GGCGGAAAAA CTGACGGCGT TGGCAGATAA TCATTATTTG 751 TTGTTCCGTT CCAATATCGG CGGCACGAGC TATTATTTCA GTAAAAAATC 801 AGCTTATGAC GACGGGTTCG GCAGAGCGTA TTTGGGTTGG CAGTATAAAA 851 ATGCACGGCA GACGGCGGGG ATTTTGCCGT TTTATCAGGT GCAGTTGTCG 901 GGCAGCGACG GCTTTGATGC GAAAACAAAA CGGGTAAACA ACCGCCGCCT 951 GCCGCCGTAT ATGCTGGCGC ACGGAGTCGG CGTGCAGTTG TCCCATACTT 1001 ACCGCCCAAA CCCGGGATGG CAATTTTCGG TCGCGCTGGA ACATTACCGC 1051 CAACGCTACC GCGAACAGGA TAGGGCGGAA TACAATAACG GTCGGCAGGA 1101 CGGGTTTTAT GTTTCGTCGG CAAAACGTTT GGGCGAATCG GCAACTGTGT 1151 TCGGCGGCTG GCAGTTTGTG CGGTTTGTGC CGAAACGCGA AACGGTGGGC 1201 GGCGCGGTCA ATAATGCCGC CTACCGGCGC AACGGTGTTT ATGCCGGCTG 1251 GGCGCAGGAG TGGCGGCAGT TGGGCGGTTT GAACAGTCGG GTTTCCGCGT 1301 CTTATGCCCG CCGCAACTAT AAGGGCGTTG CGGCTTTCTC GACAGAGGCG 1351 CAACGCAACC GCGAATGGAA TGTCTCGCTG GCTTTGAGCC ACGACAAGTT 1401 GTCGTACAAA GGTATCGTGC CCGCGTTGAA TTATCGTTTC GGCAGGACGG 1451 AAAGTAATGT GCCGTATGCG AAACGCCGCA ACAGCGAGGT GTTTGTGTCG 1501 GCGGATTGGC GGTTTTGA This corresponds to the amino acid sequence <SEQ ID 2874; ORF 935.a>: a935. pep 1 MLYFRYGFLV VWCAAGVSAA YGADAPAILD DKALLQVQRS VSDKWAESDW 51 KVDNDAPRW DGDFLLAHPK MLEHSLRDVL NGNQADLIAS LADLYAKLPD 101 YDAVLYGRAR ALLAKLAGRP AEAVARYREL HGENAADERI LLDLAAAEFD 151 DFRLKSAERH FAEAEKLDLP APVLENVGRF RKKAEGLTGW RFSGGISPAV 201 NRNANNAAPQ YCRQNGGRQI CSVSRAERAA GLNYEIEAEK LTALADNHYL 251 LFRSNIGGTS YYFSKKSAYD DGFGRAYLGW QYKNARQTAG ILPFYQVQLS 301 GSDGFDAKTK RVNNRRLPPY MLAHGVGVQL SHTYRPNPGW QFSVALEHYR 351 QRYREQDRAE YNNGRQDGFY VSSAKRLGES ATVFGGWQFV RFVPKRETVG 401 GAVNNAAYRR NGVYAGWAQE WRQLGGLNSR VSASYARRNY KGVAAFSTEA 451 QRNREWNVSL ALSHDKLSYK GIVPALNYRF GRTESNVPYA KRRNSEVFVS 501 ADWRF* m935/a935 98.8% identity in 505 aa overlap 10 20 30 40 50 60 m935 . pep MLYFRYGFLWWCAAGVSAAYGADAPAILDDKALLQVQRSVSDKWAESDWKVENDAPRW I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I a935 MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALLQVQRSVSDKWAESDWKVDNDAPRW 10 20 30 40 50 60 70 80 90 100 110 120 m935. pep DGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYDAVLYGRARALLAKLAGRP II II I II II II I I II I II M I II I I II I I II I I I I I I II II II I I I II II I I I I I II II I a935 DGDFLLAHPKMLEHSLRDVLNGNQADLIASLADLYAKLPDYDAVLYGRARALLAKLAGRP 70 80 90 100 110 120 130 140 150 160 170 180 m935. pep AEAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERHFAEAAKLDLPAPVLENVGRF II II II II II II I II II I II I II I I I II I I I I I I I I I I I II II I I II I II I II II I II I a935 AEAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERHFAEAEKLDLPAPVLENVGRF 130 140 150 160 170 180 190 200 210 220 230 240 m935. pep RKKTEGLTGWRFSGGISPAVNRNANNAAPQYCRQNGGRQICSVSRAERAAGLNYEIEAEK II I : II II II II II II II II II II II II II I II II II II II II I II II II II I I II I I I I a935 RKKAEGLTGWRFΞGGISPAVNRNANNAAPQYCRQNGGRQICSVSRAERAAGLNYEIEAEK 190 200 210 220 230 240 250 260 270 280 290 300 m935.pep LTPLADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRAYLGWQYKNARQTAGILPFYQVQLS II II M II I II I II I II II II I II I II I I I II I II I II II I II II I II I I II II II I I I a935 LTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRAYLGWQYKNARQTAGILPFYQVQLS 250 260 270 280 290 300 1348 g935.pep not found yet The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2871>: m935. seq 1 ATGTTGTATT TCAGATACGG CTTTTTGGTT GTTTGGTGTG CGGCAGGTGT 51 TTCTGCCGCC TATGGGGCGG ATGCGCCCGC GATTTTGGAT GACAAGGCAT 101 TGTTGCAGGT GCAGCGGTCG GTGTCGGATA AGTGGGCGGA ATCAGATTGG 151 AAAGTTGAAA ATGATGCCCC GCGCGTGGTT GACGGGGATT TTTTGTTGGC 201 GCATCCGAAA ATGTTGGAAC ATAGTTTGCG CGACGCGCTC AACGGCAATC 251 AGGCGGATTT AATCGCTTCG TTGGCGGATT TGTATGCCAA GCTGCCGGAT 301 TATGACGCGG TTTTGTACGG CAGGGCGCGG GCTTTGCTGG CGAAATTGGC 351 GGGAAGGCCG GCGGAGGCGG TGGCGCGGTA TCGGGAACTG CACGGGGAAA 401 ATGCGGCAGA CGAGCGGATT TTGCTGGATT TGGCGGCGGC GGAGTTTGAC 451 GATTTCCGGC TGAAGTCGGC AGAAAGGCAT TTTGCGGAGG CGGCAAAATT 501 GGATTTGCCG GCACCGGTTT TGGAAAATGT GGGGCGTTTT CGGAAAAAAA 551 CGGAGGGGCT GACGGGCTGG CGTTTTTCGG GCGGCATCAG TCCGGCGGTC 601 AATAGAAATG CCAATAATGC CGCGCCGCAA TATTGCCGGC AAAACGGAGG 651 CCGGCAGATA TGCAGTGTCA GCCGGGCGGA GCGGGCGGCA GGGTTGAATT 701 ATGAAATCGA GGCGGAAAAG CTGACGCCGT TGGCAGATAA TCATTATTTG 751 TTGTTCCGTT CCAATATCGG CGGCACGAGC TATTATTTCA GTAAAAAATC 801 AGCTTATGAT GACGGGTTCG GCAGGGCGTA TTTGGGTTGG CAGTATAAAA 851 ATGCACGGCA GACGGCGGGG ATTTTGCCGT TTTATCAGGT GCAGTTGTCG 901 GGCAGCGACG GCTTTGATGC GAAAACAAAA CGGGTAAACA ACCGCCGCCT 951 GCCGCCGTAT ATGCTGGCGC ACGGAGTCGG CGTGCAGCTG TCCCATACTT 1001 ACCGCCCAAA CCCGGGATGG CAATTTTCGG TCGCGCTGGA ACATTACCGC 1051 CAACGCTACC GCGAACAGGA TAGGGCGGAA TACAATAACG GCAGGCAGGA 1101 CGGGTTTTAT GTTTCGTCGG CAAAACGTTT GGGCGAATCG GCAACTGTGT 1151 TCGGCGGCTG GCAGTTTGTG CGGTTTGTGC CGAAACGCGA AACGGTGGGC 1201 GGCGCGGTCA ATAATGCCGC CTACCGGCGC AACGGTGTTT ATGCCGGTTG 1251 GGCGCAGGAG TGGCGGCAGT TGGGCGGTTT GAACAGTCGG GTTTCCGCGT 1301 CTTATGCCCG CCGCAACTAT AAGGGCATTG CGGCTTTCTC GACAGAGGCG 1351 CAACGCAACC GCGAATGGAA TGTCTCGCTG GCTTTGAGCC ACGACAAGTT 1401 GTCGTACAAA GGTATCGTGC CGGCGTTGAA TTATCGTTTC GGCAGGACGG 1451 AAAGTAATGT GCCGTATGCG AAACGCCGCA ACAGCGAGGT GTTTGTGTCG 1501 GCGGATTGGC GGTTTTGA This corresponds to the amino acid sequence <SEQ ID 2872; ORF 935>: m935.pep 1 MLYFRYGFLV VWCAAGVSAA YGADAPAILD DKALLQVQRS VSDKWAESDW 51 KVENDAPRW DGDFLLAHPK MLEHSLRDAL NGNQADLIAS LADLYAKLPD 101 YDAVLYGRAR ALLAKLAGRP AEAVARYREL HGENAADERI LLDLAAAEFD 151 DFRLKSAERH FAEAAKLDLP APVLENVGRF RKKTEGLTGW RFSGGISPAV 201 NRNANNAAPQ YCRQNGGRQI CSVSRAERAA GLNYEIEAEK LTPLADNHYL 251 LFRSNIGGTS YYFSKKSAYD DGFGRAYLGW QYKNARQTAG ILPFYQVQLS 301 GSDGFDAKTK RVNNRRLPPY MLAHGVGVQL SHTYRPNPGW QFSVALEHYR 351 QRYREQDRAE YNNGRQDGFY VSSAKRLGES ATVFGGWQFV RFVPKRETVG 401 GAVNNAAYRR NGVYAGWAQE WRQLGGLNSR VSASYARRNY KGIAAFSTEA 451 QRNREWNVSL ALSHDKLSYK GIVPALNYRF GRTESNVPYA KRRNSEVFVS 501 ADWRF* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2873>: a935. seq 1 ATGTTGTATT TCAGATACGG TTTTTTGGTT GTTTGGTGTG CGGCAGGTGT 51 TTCTGCCGCC TATGGGGCGG ATGCGCCCGC GATTTTGGAT GACAAGGCAT 101 TGTTGCAGGT GCAGCGGTCG GTGTCGGATA AGTGGGCGGA ATCGGATTGG 151 AAAGTTGACA ATGATGCCCC GCGCGTGGTT GACGGGGATT TTTTGTTGGC 201 GCATCCGAAA ATGTTGGAAC ATAGTTTGCG CGACGTGCTC AACGGCAATC 251 AGGCGGATTT GATCGCTTCG TTGGCGGATT TGTATGCCAA GCTGCCGGAT 301 TATGACGCGG TTTTGTACGG CAGGGCGCGG GCTTTGCTGG CGAAATTGGC 351 GGGAAGGCCG GCGGAGGCGG TGGCGCGGTA TCGGGAACTG CACGGGGAAA 401 ATGCGGCAGA CGAGCGGATT TTGCTGGATT TGGCGGCGGC GGAGTTTGAC 451 GATTTCCGGC TGAAGTCGGC AGAAAGGCAT TTTGCCGAGG CGGAAAAATT 501 GGATTTGCCG GCGCCGGTTT TGGAAAATGT GGGGCGTTTT CGGAAAAAAG 551 CGGAGGGGCT GACGGGCTGG CGTTTTTCGG GCGGCATCAG TCCGGCGGTC 310 320 330 340 350 360 m935. pep GSDGFDAKTKRVNNRRLPPYMLAHGVGVQLSHTYRPNPGWQFSVALEHYRQRYREQDRAE II I I II II I II II I II II II II I II I II II II II I II II I I I I II II I I II II II I II II a935 GSDGFDAKTKRVNNRRLPPYMLAHGVGVQLSHTYRPNPGWQFSVALEHYRQRYREQDRAE 310 320 330 340 350 360 370 380 390 400 410 420 m935.pep YNNGRQDGFYVSSAKRLGESATVFGGWQFVRFVPKRETVGGAVNNAAYRRNGVYAGWAQE II I I II II I II I II I I II II II II II II I II I I I I II II I II I II II I II II II II II II a935 YNNGRQDGFYVSSAKRLGESATVFGGWQFVRFVPKRETVGGAVNNAAYRRNGVYAGWAQE 370 380 390 400 410 420 430 440 450 460 470 480 m935. pep WRQLGGLNSRVSASYARRNYKGIAAFSTEAQRNREWNVSLALSHDKLSYKGIVPALNYRF II II II II I I II I II II II I MM II II I I II II I II II II II II I I I II I I II II II II a935 WRQLGGLNSRVSASYARRNYKGVAAFSTEAQRNREWNVSLALSHDKLSYKGIVPALNYRF 430 440 450 460 470 480 490 500 m935.pep GRTESNVPYAKRRNSEVFVSADWRFX II II II I II I II I I I II II II II II I a935 GRTESNVPYAKRRNSEVFVSADWRFX 490 500 The following partial DNA sequence was identified in N.gonorrhoeae <SEQ ID 2875>: g936.seq 1 ATGAAACCCA AACCACACAC CGTCCGCACC CTGATTGCCG CCGTCCTCAG 51 CCTTGCCCTC GGCGGCTGCT TCAGCGCAGT CGTCGGCGGG GCCGCCGTCG 101 GCGCAAAATC CGTCATCGAC CGccgAACCA CCGgcgcgca AACCGATGac 151 aACGTTATGG CGTTGCGTAT CGAAACCACC GCCCGTTCCT ACCTGCGCCA 201 AAACAACCAA ACCAAAGGCT ACACGCCCCA AATCTCCGTC GTCGGCTACA 251 ACCGCCACCT GCTGCTGCTC GGACAAGTCG CCACCGAAGG CGAAAAACAG 301 TTCGTCGGTC AGATTGCACG TTCCGAACAG GCCGCCGAAG GCGTATACAA 351 CTACATTACC GTCGCCTCCC TGCCGCGCAC TGCGGGCGAC ATCGCCGGCG 401 ACACTTGGAA CACGTCCAAA GTCCGCGCca cgCTGCTGGG CATCAGCCCC 451 GCTACACAGG CGCGCGTCAA AATCATTACC TACGGCAATG TAACCTACGT 501 TATGGGCATC CTCACCCCCG AAGAACAGGC GCAGATTACC CAAAAAGTCA 551 GCACCAccgT CGGCGTACAA AAAGTCATTA CCCTCTACCA AAACTACGTC 601 CAACGCTGA This corresponds to the amino acid sequence <SEQ ID 2876; ORF 936.ng>: g936.pep 1 MKPKPHTVRT LIAAVLSLAL GGCFSAWGG AAVGAKSVID RRTTGAQTDD 51 NVMALRIETT ARSYLRQNNQ TKGYTPQISV VGYNRHLLLL GQVATEGEKQ 101 FVGQIARSEQ AAEGVYNYIT VASLPRTAGD IAGDTWNTSK VRATLLGISP 151 ATQARVKIIT YGNVTYVMGI LTPEEQAQIT QKVSTTVGVQ KVITLYQNYV 201 QR* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2877>: m936.seq (partial) 1 ATGAAACCCA AACCGCACAC CGTCCGCACC CTGATTGCCG CCATTTTCAG 51 CCTTGCCCTT AGCGGCTGCG TCAGCGCAGT AATCGGAAGC GCCGCCGTCG 101 GCGCGAAATC CGCCGTCGAC CGCCGAACCA CCGGCGCGCA AACCGACGAC 151 AACGTTATGG CGTTGCGTAT CGAAACCACC GCCCGTTCCT ATCTGCGCCA 201 AAACAACCAA ACCAAAGGCT ACACGCCCCA AATCTCCGTC GTCGGCTACA 251 ACCGCCACCT GCTGCTGCTC GGACAAGTCG CCACCGAAGG CGAAAAACAG 301 TTCGTCGGTC AGATTGCACG TTCCGAACAG GCCGCCGAAG GCGTGTACAA 351 CTATATTACC GTCGCCTCCC TGCCGCGCAC TGCC... This corresponds to the amino acid sequence <SEQ ID 2878; ORF 936>: m936.pep (partial) 1 MKPKPHTVRT LIAAIFSLAL SGCVSAVIGS AAVGAKSAVD RRTTGAQTDD 51 NVMALRIETT ARSYLRQNNQ TKGYTPQISV VGYNRHLLLL GQVATEGEKQ 101 FVGQIARSEQ AAEGVYNYIT VASLPRTA... Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 936 shows 93.8% identity over a 128 aa overlap with a predicted ORF (ORF 936.ng) from N gonorrhoeae: m936/g936 10 20 30 40 50 60 m936. pep MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTTGAQTDDΝVMALRIETT M M I M M M I I h M l l h l l I I I = 1 = I I I I I I h = I I I I I I I I I I I I I I I I I I I I I g936 MKPKPHTVRTLIAAVLSLALGGCFSAWGGAAVGAKSVIDRRTTGAQTDDΝVMALRIETT 10 20 30 40 50 60 70 80 90 100 110 120 m936. pep ARSYLRQΝΝQTKGYTPQISWGYΝRHLLLLGQVATEGEKQFVGQIARSEQAAEGVYΝYIT MMMMMMMMMMMMMMMMMMMMMMMMMIMIMMM g936 ARSYLRQΝΝQTKGYTPQISWGYΝRHLLLLGQVATEGEKQFVGQIARSEQAAEGVYΝYIT 70 80 90 100 110 120 130 m936. pep VASLPRTAXXX g936 VASLPRTAGDIAGDTWΝTSKVRATLLGISPATQARVKIITYGΝVTYVMGILTPEEQAQIT 130 140 150 160 170 180 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2879>: a936. seq 1 ATGAAACCCA AACCGCACAC CGTCCGCACC CTGACTGCCG CCGTCCTCAG 51 CCTTGCCCTC GGCGGCTGCG TCAGCGCAGT CGTCGGCGGC GCGGCGGTCG 101 GCGCGAAATC CGCCGTCGAC CGCCGAACCA CCGGCGCGCA AACCGACGAC 151 AACGTAATGG CGCTGCGTAT CGAAACCACC GCCCGCTCCT ATCTGCGCCA 201 AAACAACCAA ACCAAAGGCT ACACGCCCCA AATCTCCGTT GTCGGCTACA 251 ACCGCCACCT GCTGCTGCTC GGACAAGTCG CCACCGAAGG CGAGAAACAG 301 TTCGTCGGTC AGATTGCACG TTCCGAACAG GCCGCCGAAG GCGTGTACAA 351 CTACATTACC GTCGCCTCCC TGCCGCGCAC TGCCGGCGAC ATCGCCGGCG 401 ACACTTGGAA CACATCCAAA GTCCGCGCCA CGCTGTTGGG CATCAGCCCC 451 GCCACACAGG CGCGCGTCAA AATCGTTACC TACGGCAACG TAACCTACGT 501 TATGGGCATC CTCACCCCCG AAGAACAGGC GCAGATTACC CAAAAAGTCA 551 GCACCACCGT CGGCGTACAA AAAGTCATCA CCCTCTACCA AAACTACGTC 601 CAACGCTGA This corresponds to the amino acid sequence <SEQ ID 2880; ORF 936. a>: a936.pep 1 MKPKPHTVRT LTAAVLSLAL GGCVSAWGG AAVGAKSAVD RRTTGAQTDD 51 NVMALRIETT ARSYLRQNNQ TKGYTPQISV VGYNRHLLLL GQVATEGEKQ 101 FVGQIARSEQ AAEGVYNYIT VASLPRTAGD IAGDTWNTSK VRATLLGISP 151 ATQARVKIVT YGNVTYVMGI LTPEEQAQIT QKVSTTVGVQ KVITLYQNYV 201 QR* m936/a936 95.3% identity in 128 aa overlap 10 20 30 40 50 60 ιrι936 . pep MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTTGAQTDDNVMALRIETT I I I I I I I I I I I I I : : I I I M I I I I I M I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a 936 MKPKPHTVRTLTAAVLSLALGGCVSAWGGAAVGAKSAVDRRTTGAQTDDNVMALRIETT 10 20 30 40 50 60 70 80 90 100 110 120 m936.pep ARSYLRQNNQTKGYTPQISWGYNRHLLLLGQVATEGEKQFVGQIARSEQAAEGVYNYIT II II I II II I II I II I I I I I I II II II M II M I II II I I I I M I II I M I I M II II I I a936 ARSYLRQNNQTKGYTPQISWGYNRHLLLLGQVATEGEKQFVGQIARSEQAAEGVYNYIT 70 80 90 100 110 120 m936.pep VASLPRTA a936 VASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVTYGNVTYVMGILTPEEQAQIT 130 140 150 160 170 180 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2881>: g936-l.seq i 1 1 ATGAAACCCA AACCACACAC CGTCCGCACC CTGATTGCCG CCGTCCTCAG 51 CCTTGCCCTC GGCGGCTGCT TCAGCGCAGT CGTCGGCGGG GCCGCCGTCG 101 GCGCAAAATC CGTCATCGAC CGccgAACCA CCGgcgcgca AACCGATGac 151 aACGTTATGG CGTTGCGTAT CGAAACCACC GCCCGTTCCT ACCTGCGCCA 201 iAAACAACCAA ACCAAAGGCT ACACGCCCCA AATCTCCGTC GTCGGCTACA 251 iACCGCCACCT GCTGCTGCTC GGACAAGTCG CCACCGAAGG CGAAAAACAG 301 TTCGTCGGTC AGATTGCACG TTCCGAACAG GCCGCCGAAG GCGTATACAA 351 CTACATTACC GTCGCCTCCC TGCCGCGCAC TGCGGGCGAC ATCGCCGGCG 401 iACACTTGGAA CACGTCCAAA GTCCGCGCca cgCTGCTGGG CATCAGCCCC 451 GCTACACAGG CGCGCGTCAA AATCATTACC TACGGCAATG TAACCTACGT 501 TATGGGCATC CTCACCCCCG AAGAACAGGC GCAGATTACC CAAAAAGTCA 551 GCACCAccgT CGGCGTACAA AAAGTCATTA CCCTCTACCA AAACTACGTC 601 CAACGCTGA This corresponds to the amino acid sequence <SEQ ID 2882; ORF 936-1. ng>: g936-l.pep 1 MKPKPHTVRT LIAAVLSLAL GGCFSAVVGG AAVGAKSVID RRTTGAQTDD 51 NVMALRIETT ARSYLRQNNQ TKGYTPQISV VGYNRHLLLL GQVATEGEKQ 101 FVGQIARSEQ AAEGVYNYIT VASLPRTAGD IAGDTWNTSK VRATLLGISP 151 ATQARVKIIT YGNVTYVMGI LTPEEQAQIT QKVSTTVGVQ KVITLYQNYV 201 QR* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2883>: m936-l.seq 1 ATGAAACCCA AACCGCACAC CGTCCGCACC CTGATTGCCG CCATTTTCAG 51 CCTTGCCCTT AGCGGCTGCG TCAGCGCAGT AATCGGAAGC GCCGCCGTCG 101 GCGCGAAATC CGCCGTCGAC CGCCGAACCA CCGGCGCGCA AACCGACGAC 151 AACGTTATGG CGTTGCGTAT CGAAACCACC GCCCGTTCCT ATCTGCGCCA 201 AAACAACCAA ACCAAAGGCT ACACGCCCCA AATCTCCGTC GTCGGCTACA 251 ACCGCCACCT GCTGCTGCTC GGACAAGTCG CCACCGAAGG CGAAAAACAG 301 TTCGTCGGTC AGATTGCACG TTCCGAACAG GCCGCCGAAG GCGTGTACAA 351 CTATATTACC GTCGCCTCCC TGCCGCGCAC TGCCGGCGAC ATCGCCGGCG 401 ACACTTGGAA CACATCCAAA GTCCGCGCCA CGCTGTTGGG CATCAGCCCC 451 GCCACACAGG CGCGCGTCAA AATCGTTACC TACGGCAACG TAACCTACGT 501 TATGGGCATC CTCACCCCCG AAGAACAGGC GCAGATTACC CAAAAAGTCA 551 GCACCACCGT CGGCGTACAA AAAGTCATCA CCCTCTACCA AAACTACGTC 601 CAACGCTGA This corresponds to the amino acid sequence <SEQ ID 2884; ORF 936- 1>: m936-l.pep 1 MKPKPHTVRT LIAAIFSLAL SGCVΞAVIGS AAVGAKSAVD RRTTGAQTDD 51 NVMALRIETT ARSYLRQNNQ TKGYTPQISV VGYNRHLLLL GQVATEGEKQ 101 FVGQIARSEQ AAEGVYNYIT VASLPRTAGD IAGDTWNTSK VRATLLGISP 151 ATQARVKIVT YGNVTYVMGI LTPEEQAQIT QKVSTTVGVQ KVITLYQNYV 201 QR* m936-l/g936-l 95.5% identity in 202 aa overlap 10 20 30 40 50 60 m936-l.pep MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTTGAQTDDNVMALRIETT I I I I I I I I I I I I I MM I I M I I II M I M I I I I I M : I I I I I I I M I I I I I I I I I I I I g936-l MKPKPHTVRTLIAAVLSLALGGCFSAVVGGAAVGAKSVIDRRTTGAQTDDNVMALRIETT 10 20 30 40 50 60 70 80 90 100 110 120 m936-l.pep ARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFVGQIARSEQAAEGVYNYIT I I I II I II I I I I I I I M II I I I I I I I I II I I I I II I I I I I I I II I I I I I I I I I II I I I M g936-l ARSYLRQNNQTKGYTP Q ISVVGYNRHLLLLGQVATEGEKQFVGQIARSEQAAEGVYNYIT 70 80 90 100 110 120 130 140 150 160 170 180 m936-l.pep VASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVTYGNVTYVMGILTPEEQAQIT I I I II I I II I I I I I I II I I I I I I I II I I II 1 I I I I I II :| I I I I I I I I I I I I II II I I i I g936-l VASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIIT GNVTYVMGILTPEEQAQIT 130 140 150 160 170 180 190 200 m936-l .pep QKVSTTVGVQKVITLYQNYVQRX I I I I I I I I I II I I I I I I I I I I I I g936-l QKVSTTVGVQKVITLYQNYVQRX 190 200 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2885>: a936-l.seq 1 ATGAAACCCA AACCGCACAC CGTCCGCACC CTGACTGCCG CCGTCCTCAG 51 CCTTGCCCTC GGCGGCTGCG TCAGCGCAGT CGTCGGCGGC GCGGCGGTCG 101 GCGCGAAATC CGCCGTCGAC CGCCGAACCA CCGGCGCGCA AACCGACGAC 151 AACGTAATGG CGCTGCGTAT CGAAACCACC GCCCGCTCCT ATCTGCGCCA 201 AAACAACCAA ACCAAAGGCT ACACGCCCCA AATCTCCGTT GTCGGCTACA 251 ACCGCCACCT GCTGCTGCTC GGACAAGTCG CCACCGAAGG CGAGAAACAG 301 TTCGTCGGTC AGATTGCACG TTCCGAACAG GCCGCCGAAG GCGTGTACAA 351 CTACATTACC GTCGCCTCCC TGCCGCGCAC TGCCGGCGAC ATCGCCGGCG 401 ACACTTGGAA CACATCCAAA GTCCGCGCCA CGCTGTTGGG CATCAGCCCC 451 GCCACACAGG CGCGCGTCAA AATCGTTACC TACGGCAACG TAACCTACGT 501 TATGGGCATC CTCACCCCCG AAGAACAGGC GCAGATTACC CAAAAAGTCA 551 GCACCACCGT CGGCGTACAA AAAGTCATCA CCCTCTACCA AAACTACGTC 601 CAACGCTGA This corresponds to the amino acid sequence <SEQ ID 2886; ORF 936-1. a>: a936-l.pep 1 MKPKPHTVRT LTAAVLSLAL GGCVSAVVGG AAVGAKSAVD RRTTGAQTDD 51 NVMALRIETT ARSYLRQNNQ TKGYTPQISV VGYNRHLLLL GQVATEGEKQ 101 FVGQIARSEQ AAEGVYNYIT VASLPRTAGD IAGDTWNTSK VRATLLGISP 151 ATQARVKIVT YGNVTYVMGI LTPEEQAQIT QKVSTTVGVQ KVITLYQNYV 201 QR* a936-l/m936-l 97.0% identity in 202 aa overlap 10 20 30 40 50 60 m936-l.pep MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTTGAQTDDNVMALRIETT I II I I I II I I I I I : : I I I M I I II I I M : I I I I I I I I I I I I I I I I 11 I I I I I I I I I I I I a936-l MKPKPHTVRTLTAAVLSLALGGCVSAVVGGAAVGAKSAVDRRTTGAQTDDNVMALRIETT 10 20 30 40 50 60 70 80 90 100 110 120 m936-l.pep ARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFVGQIARSEQAAEGVYNYIT I I I II II I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I a936-l ARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFVGQIARSEQAAEGVYNYIT 70 80 90 100 110 120 130 140 150 160 170 180 m936-l.pep VASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVTYGNVTYVMGILTPEEQAQIT I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I II I II I I I I I I I I I I I I I I I I a936-l VASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVTYGNVTYVMGILTPEEQAQIT 130 140 150 160 170 180 190 200 m936-l .pep QKVSTTVGVQKVITLYQNYVQRX I I I I I I I I I I I I I I II I I I I I I I a936-l QKVSTTVGVQKVITLYQNYVQRX 190 200 The following partial DNA sequence was identified in N.gonorrhoeae <SEQ ID 2887>: g937.seq 1 atGAAAAATA TTCTCTTAgt ATTTGTTAGC TTTGTGCCAT TATGTGTCCG 51 CACTGATCTG CCGCTGAata tCGAAGACAT AATGaccgAC AAGGGAAAAT 101 GGAAactGGA AACTTccctt acctacctgA acaGCGAAAA cagCCGCGCC 151 GCACTTGCCT CACCGGTTTA CATTCAGACC GGCTCCGCTT CCTTTATCCC 201 CGTCCCGACC GAAATTCAGG AAAACGGCAG CAATACCGAT ATGCTCGCCG 251 GCACGCTCGG TTTGCGCTAC GGACTGAccg GCAataccgA CATTTACGGC 301 AGCGGCAGCT ATCTGTGGCA CGAAGAACGC AAACTCGacg GCAACGGCAA 351 AACCCGCAAC AAACGGATGT CCGACATATC CGCCGGCATC AGCCACACCT 401 TCCttaAAGa cgGCAAAAAT CCCGCACTCA TCGCTTTCCT CGAAAGCACG 451 GTTTACGAAA AATCGCGCAA CAAAGCCTCG TCGGGAAAAT CGTGGCTCAT 501 CGGCGCCACC ACCTACAAAG CCATAGATCC GATTGTCCTT TCCCTCACCG 551 CCGCCTACCG CATCAACGGC AGCAAAACCC TTTCAGACGA CGTCAAATAC 601 AAAGCAGGCA ATTACTGGAT GCTGAATCCC AACATCTCAT TTGCCGCCAA 651 CGACAGAATC AGCCTGACCG GAGGCATCCA ATGGCTGGGC AAACAGCCCG 701 ACCGCATAGA CGGCAAAAAA GAATCCGCAA GAAACACATC CACCTACGCC 751 CATTTCGGCG CAGGTTTCGG TTTCACCAAA ACCGCGGCTT TAAACGCATC 801 CGCACGTTTC AACGTTTCAG GGCAAAGCAG TTCCGAACTG AAATTGGGCG 851 TACAGCATAC ATTTTAA This corresponds to the amino acid sequence <SEQ ID 2888; ORF 937.ng>: g937.pep 1 MKNILLVFVS FVPLCVRTDL PLNIEDIMTD KGKWKLETSL TYLNSENSRA 51 ALASPVYIQT GSASFIPVPT EIQENGSNTD MLAGTLGLRY GLTGNTDIYG 101 SGSYLWHEER KLDGNGKTRN KRMSDISAGI SHTFLKDGKN PALIAFLEST 151 VYEKSRNKAS SGKSWLIGAT TYKAIDPIVL SLTAAYRING SKTLSDDVKY 201 KAGNYWMLNP NISFAANDRI SLTGGIQWLG KQPDRIDGKK ESARNTSTYA 251 HFGAGFGFTK TAALNASARF NVSGQSSSEL KLGVQHTF* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2889>: m937.seq 1 ATGAAGCGCA TCTTTTTGCC CGCCTTGCCC GCCATCCTGC CTTTATCCAC 51 TTATGCCGAC CTGCCCTTGA CGATTGAAGA CATAATGACC GACAAGGGAA 101 AATGGAAACT GGAAACTTCC CTTACCTACC TGAACAGCGA AAACAACCGC 151 GCCGAACTTG CCGCACCGGT TTACATTCAA ACCGGCGCAA CCTCGTTTAT 201 CCCCATTCCG ACCGAAATCC AAgAAA CGG CAGCAATACC GATATGCTCG 251 TCGGCACGCT CGGTTTGCGC TACGGACTGA CCGGGAATAC CGACATTTAC 301 GGCAGCGGCA GCTATCTGTG GCACGAAGAA CGCAAACTCG ACGGCAACAG 351 CAAAACCCGC AACAAACGGA TGTCCGACGT ATCCCTCGGC ATCAGCCACA 401 CTTTCCTTAA AGACGACAAA AACCCCGCCC TAATCAGCTT TCTTGAAAGC 451 ACGGTTTACG AAAAATCGCG CAACAAAGCC TCGTCGGGAA AATCCTGGCT 501 CATCGGCGCC ACCACCTACA AAGCCATAGA TCCGATTGTC CTTTCCCTCA 551 CCGCCGCCTA CCGCATCAAC GGCAGCAAAA CCCTTTCAGA CGGCATCCGC 601 TACAAATCGG GCAACTACCT GCTGCTCAAC CCCAACATCT CATTTGCTGC 651 CAACGACAGA ATCAGCCTGA CCGGAGGCAT CCAATGGCTG GGCAGGCAGC 701 CCGACCGGAC GGACGGCAAA CGGGAATCCT CCAGAAACAC ATCCACCTAC 751 GCCCATTTCG GCGCAGGTTT CGGTTTCACC AAAACCACGG CTTTAAACGC 801 ATCCGCACGT TTCAACGTTT CAGGGCAAAG CAGTTCCGAA CTGAAATTTG 851 GCGTACAGCA TACATTTTAA This corresponds to the amino acid sequence <SEQ ID 2890; ORF 937>: m937.pep.. 1 MKRIFLPALP AILPLSTYAD LPLTIEDIMT DKGKWKLETS LTYLNSENNR 51 AELAAPVYIQ TGATSFIPIP TEIQENGSNT DMLVGTLGLR YGLTGNTDIY 101 GSGSYLWHEE RKLDGNSKTR NKRMSDVSLG ISHTFLKDDK NPALISFLES 151 TVYEKSRNKA SSGKSWLIGA TTYKAIDPIV LSLTAAYRIN GSKTLSDGIR 201 YKSGNYLLLN PNISFAANDR ISLTGGIQWL GRQPDRTDGK RESSRNTSTY 251 AHFGAGFGFT KTTALNASAR FNVSGQSSSE LKFGVQHTF* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N.gonorrhoeae ORF 937 shows 86.9% identity over a 289 aa overiap with a predicted ORF (ORF 937.ng) from N gonorrhoeae: g937/m937 10 20 30 40 50 59 g937.pep MKΝILL-VFVSFVPLCVRTDLPLΝIEDIMTDKGKWKLETSLTYLΝSEΝΞRAALASPVYIQ I I M l = = = = = l l = = 1 1 1 1 = 1 1 1 1 1 1 1 1 1 1 I I l l l l M M I I I M M 1 1 = 1 1 I I I m937 MKRIFLPALPAILPLSTYADLPLTIEDIMTDKGKWKLETSLTYLΝSEΝΝRAELAAPVYIQ 10 20 30 40 50 60 60 70 80 90 100 110 119 g937.pep TGSASFIPVPTEIQEΝGSΝTDMLAGTLGLRYGLTGΝTDIYGΞGSYLWHEERKLDGΝGKTR m937 TGATSFIPIPTEIQENGSNTDMLVGTLGLRYGLTGNTDIYGSGSYLWHEERKLDGNSKTR 70 80 90 100 110 120 120 130 140 150 160 170 179 g937 pep NKRMSDISAGISHTFLKDGKNPALIAFLESTVYEKSRNKASSGKSWLIGATTYKAIDPIV Mlllhl MIIIIMI 11 II 11 = II II II II II II II II I II II II II I II II II II m 937 NKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKASSGKSWLIGATTYKAIDPIV 130 140 150 160 170 180 180 190 200 210 220 230 239 g937 pep LSLTAAYRINGSKTLSDDVKYKAGNYWMLNPNISFAANDRISLTGGIQWLGKQPDRIDGK = = l h l l l M M M M M M M M I M M M h M M M l m 937 LSLTAAYRINGSKTLSDGIRYKSGNYLLLNPNISFAANDRISLTGGIQWLGRQPDRTDGK 190 200 210 220 230 240 240 250 260 270 280 289 g937. pep KESARNTSTYAHFGAGFGFTKTAALNASARFNVSGQSSSELKLGVQHTFX M h M M M M M M M M I h M M M M M M M M I I h l M M M m937 RESSRNTSTYAHFGAGFGFTKTTALNASARFNVSGQSSSELKFGVQHTFX 250 260 270 280 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2891>: a937. seq 1 ATGAAGCGCA TCTTTTTGCC CGCCTTGCCC GCCATCCTGC CTTTATCCGC 51 TTATGCCGAC CTGCCCTTGA CGATTGAAGA CATAATGACC GACAAGGGCA 101 AATGGAAACT GGAAACTTCC CTTACCTACC TGAACAGCGA AAACAACCGC 151 GCCGAACTTG CCGCACCGGT TTACATCCAA ACCGGCGCAA CCTCGTTTAT 201 CCCCATTCCG ACCGAAATCC AAGAAAACGG CAGCAATACC GATATGCTCG 251 TTGGCACGCT CGGTTTGCGC TACGGACTGA CCGGGAATAC CGACATTTAC 301 GGCAGCGGCA GCTATCTGTG GCACGAAGAA CGCAAACTCG ACGGCAACGG 351 CAAAACCCGA AACAAACGGA TGTCCGACGT ATCCCTCGGC ATCAGCCACA 401 CCTTCCTTAA AGACGACAAA AACCCCGCCC TAATCAGCTT TCTTGAAAGC 451 ACGGTTTACG AAAAATCGCG CAACAAAGCC TCGTCGGGAA AATCCTGGCT 501 CATCGGCGCC ACCACCTACA AAGCCATCGA CCCCGTCGTC CTCTCATTGA 551 CCGCTGCCTA CCGTATCAAC GGCAGCAAAA CCCTTTCAAG CAACACCAAA 601 TACAAAGCAG GCAATTACTG GATGCTGAAT CCCAATATAT CCTTCGCCGC 651 CAACGACAGA ATCAGCCTCA CGGGCGGCAT CCAATGGCTG GGCAAGCAGC 701 CCGACCGTCT GGACGGCAAA AAAGAATCCG CAAGAAACAC ATCCACCTAT 751 GCCCATTTCG GCGCAGGTTT CGGTTTCACC AAAACCACGG CTTTAAACGC 801 ATCCGCACGT TTCAACGTTT CAGGGCAAAG CAGTTCCGAA CTGAAATTTG 851 GCGTACAGCA TACGTTTTAA This corresponds to the amino acid sequence <SEQ ID 2892; ORF 937. a>: a937.pep 1 MKRIFLPALP AILPLSAYAD LPLTIEDIMT DKGKWKLETS LTYLΝSEΝΝR 51 AELAAPVYIQ TGATSFIPIP TEIQEΝGSΝT DMLVGTLGLR YGLTGΝTDIY 101 GSGSYLWHEE RKLDGΝGKTR ΝKRMSDVSLG ISHTFLKDDK ΝPALISFLES 151 TVYEKSRΝKA SSGKSWLIGA TTYKAIDPW LSLTAAYRIΝ GSKTLSSΝTK 201 YKAGΝYWMLΝ PΝISFAAΝDR ISLTGGIQWL GKQPDRLDGK KESARΝTSTY 251 AHFGAGFGFT KTTALΝASAR FΝVSGQSSSE LKFGVQHTF* m937/a937 95.2% identity in 289 aa overlap 10 20 30 40 50 60 m937. pep MKRIFLPALPAILPLSTYADLPLTIEDIMTDKGKWKLETSLTYLΝSEΝΝRAELAAPVYIQ II I II II II II II II M I II I II II II II II M II II I II I I II II II II I M II I I I I I a 937 MKRIFLPALPAILPLSAYADLPLTIEDIMTDKGKWKLETSLTYLΝSEΝΝRAELAAPVYIQ 10 20 30 40 50 60 70 80 90 100 110 120 m937. pep TGATSFIPIPTEIQEΝGSΝTDMLVGTLGLRYGLTGΝTDIYGSGSYLWHEERKLDGΝSKTR II I I I II I I II II II II I I I II I I I I I I II II I I II I I I II II I I I I I I I I I I I I I : M I a 937 TGATSFIPIPTEIQEΝGSΝTDMLVGTLGLRYGLTGΝTDIYGSGSYLWHEERKLDGΝGKTR 70 80 90 100 110 120 130 140 150 160 170 180 m937.pep NKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKASSGKSWLIGATTYKAIDPIV M I II I II II II II II M II II II II II I II I II I I II I I II II I I II II M I I I M M I a937 NKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKASSGKSWLIGATTYKAIDPVV 130 140 150 160 170 18C 190 200 210 220 230 24C m93 .pep LSLTAAYRINGSKTLSDGIRYKSGNYLLLNPNISFAANDRISLTGGIQWLGRQPDRTDGK I I I I I I I I I I I I I I I I : : : I I : I I I M M M M M M M M M M M I M M M I I I a937 LSLTAAYRINGSKTLSSNTKYKAGNYWMLNPNISFAANDRISLTGGIQWLGKQPDRLDGK 190 200 210 220 230 240 250 260 270 280 290 m937.pep RESSRNTSTYAHFGAGFGFTKTTALNASARFNVSGQSSSELKFGVQHTFX : I I : I II I II II I II II II II II II I II I I II II I II I II II II II II Ii a937 KESARNTSTYAHFGAGFGFTKTTALNASARFNVSGQSSSELKFGVQHTFX 250 260 270 280 290 g939.seq not found yet g939.pep not found yet The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2893>: m939.seq (partial) 1 ATGAAACGAT TGACTTTATT GGCCTTTGTT TTGGCTGCCG GTGCGGTTTC 51 CGCCTCTCCC AAAGCAGACG TGGAAAAAGG CAAACAGGTT GCCGCAACGG 101 TTTGTGCGGC TTGCCATGCA GCAGACGGTA ACAGCGGCAT TGCGATGTAT 151 CCGCGTTTGG CGGCACAGCA TACTGCTTAC ATCTATCATC AAACTATCGG 201 CATCCGCGAC GTAAACGCAC CC ... This corresponds to the amino acid sequence <SEQ ID 2894; ORF 939>: m939.pep (partial) 1 MKRLTLLAFV LAAGAVSASP KADVEKGKQV AATVCAACHA ADGNSGIAMY 51 PRLAAQHTAY IYHQTIGIRD VNAP ... The following partial DNA sequence was identified in N meningitidis <SEQ ID 2895>: a939.seq 1 ATGAAACGAT TGACTTTATT GGCCTTTGTT TTGGCTGCCG GTGCGGTTTC 51 CGCATCTCCC AAAGCAGACG TGGAAAAAGG CAAACAGGTT GCCGCAACGG 101 TTTGTGCGGC TTGCCATGCA GCAGACGGTA ACAGCGGCAT TGCGATGTAT 151 CCGCGTTTGG CGGCACAGCA TACTGCTTAC ATCTATCATC AAACCATCGG 201 CATCCGCGAC GGTAAACGCA CCCACGGTTC GGCAGCTGTG ATGAAACCGG 251 TGGTAATGAA TTTGAGCGAT CAGGATATTT TGAACGTATC CGCATTCTAT 301 GCCAAACAGC AGCCCAAATC CGGTGAAGCC AATCCTAAGG AAAATCCCGA 351 ATTGGGTGCG AAAATCTATC GCGGCGGTTT GAGCGATAAA AAAGTGCCGG 401 CGTGTATGTC CTGCCACGGT CCGAGCGGTG CGGGTATGCC GGGGGGCGGA 451 AGCGAAATTC AGGCTTATCC GCGTTTGGGC GGTCAGCATC AGGCATATAT 501 TGTTGAACAG ATGAATGCCT ACAAGTCCGG TCAGCGTAAA AATACCATCA 551 TGGAAGATAT TGCAAACCGT ATGTCTGAAG AAGATTTGAA AGCGGTCGCC 601 AACTTTATCC AAGGTTTGCG TTAA This corresponds to the amino acid sequence <SEQ ID 2896; ORF 939. a>: a939.pep 1 MKRLTLLAFV LAAGAVSASP KADVEKGKQV AATVCAACHA ADGNSGIAMY 51 PRLAAQHTAY IYHQTIGIRD GKRTHGSAAV MKPWMNLSD QDILNVSAFY 101 AKQQPKSGEA NPKENPELGA KIYRGGLSDK KVPACMSCHG PSGAGMPGGG 151 SEIQAYPRLG GQHQAYIVEQ MNAYKSGQRK NTIMEDIANR MSEEDLKAVA 201 NFIQGLR* m939/a939 100.0% identity in 70 aa overlap 10 20 30 40 50 60 m939. pep MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGNSGIAMYPRLAAQHTAY I II I II M I II I II I II I I M I II II II II II II II I I I I I I I I I M II II I I II II I M a939 MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGNSGI MYPRLAAQHTAY 10 20 30 4 0 50 60 70 m939 . pep IYHQTIGIRDVNAP I I I I I I I I I I a939 IYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAKQQPKSGEANPKENPELGA 70 80 90 100 110 120 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2897>: g950.seq 1 ATGAACAAAA ATATTGCTGC CGCACTCGCC GGTGCTTTAT CCCTGTCTCT 51 GGCCGCCGGC GCCGTTGCCG CCCACAAACC GGCAAGCAAC GCAACAGGCG 101 TTCAAAAATC CGCCCAAGGC TCTTGCGGCG CATCCAAATC TGCCGAAGGT 151 TCGTGCGGCG CATCCAAATC TGCCGAAGGT TCGTGCGGCG CGGCTGCTTC 201 TAAAGCAGGC GAAGGCAAAT GCGGCGAGGG CAAATGCGGT GCAACTGTAA 251 AAAAAGCCCA CAAACACACC AAAGCATCTA AAGCCAAAGC CAAATCTGCC 301 GAAGGCAAAT GCGGCGAAGG CAAATGCGGT TCTAAATAA This corresponds to the amino acid sequence <SEQ ID 2898; ORF 950.ng>: g950.pep 1 MΝKΝIAAALA GALSLSLAAG AVAAHKPASΝ ATGVQKSAQG SCGASKSAEG 51 SCGASKSAEG SCGAAASKAG EGKCGEGKCG ATVKKAHKHT KASKAKAKSA 101 EGKCGEGKCG SK* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2899>: m950.seq 1 ATGAACAAAA ACATTGCTGC CGCTCTCGCC GGTGCTTTAT CCCTGTCTTT 51 GGCCGCCGGT GCAGTTGCTG CCAACAAACC GGCAAGCAAC GCAACAGGCG 101 TTCATAAATC CGCCCATGGC TCTTGCGGCG CGTCCAAATC TGCCGAAGGT 151 TCGTGCGGCG CGGCTGGTTC TAAAGCAGGC GAAGGCAAAT GCGGCGAGGG 201 CAAATGCGGT GCGACCGTAA AAAAAACCCA CAAACACACC AAAGCATCTA 251 AAGCCAAGGC CAAATCTGCC GAAGGCAAAT GCGGCGAAGG CAAATGCGGT 301 TCTAAATAA This corresponds to the amino acid sequence <SEQ ID 2900; ORF 950>: m950.pep 1 MΝKΝIAAALA GALSLSLAAG AVAAΝKPASΝ ATGVHKSAHG SCGASKSAEG 51 SCGAAGSKAG EGKCGEGKCG ATVKKTHKHT KASKAKAKSA EGKCGEGKCG 101 SK Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 950 shows 86.6% identity over a 112 aa overlap with a predicted ORF (ORF 950) from N gonorrhoeae m950/g950 86.6% identity in 112 aa overlap 10 20 30 40 50 m950.pep MΝKΝIAAALAGALSLΞLAAGAVAAΝKPASΝATGVHKSAHGSCGASKΞAEGSCGA I I I II I I I I I I I I I I I I I II I II I M I II I I I I I : I I I : I I I I I I I I I I I I I I I g950 MΝKΝIAAALAGALSLSLAAGAVAAHKPASΝATGVQKSAQGSCGASKSAEGSCGASKSAEG 10 20 30 40 50 60 60 70 80 90 100 m950.pep AGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK M II I I I I I I I I I I I I I I I I M I I I I II I I I I I I I I I I I I I I I I I M I g950 ΞCGAAASKAGEGKCGEGKCGATVKKAHKHTKAΞKAKAKSAEGKCGEGKCGSKX 70 80 90 100 110 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2901>: a950. seq 1 ATGAACAAAA ACATTGCTGC CGCACTCGCC GGTGCTTTAT CCCTGTCTTT 51 GGCCGCCGGT GCAGTTGCTG CCAACAAACC GGCAAGCAAC GCAACAGGCG 101 TTCATAAATC CGCCCATGGC TCTTGCGGCG CGTCCAAATC TGCCGAAGGT 151 TCGTGCGGCG CGGCTGGTTC TAAAGCAGGC GAAGGCAAAT GCGGCGAGGG 201 CAAATGCGGT GCGACCGTAA AAAAAACCCA CAAACACACC AAAGCATCTA 251 AAGCCAAGGC CAAATCTGCC GAAGGCAAAT GCGGCGAAGG CAAATGCGGT 301 TCTAAATAA This corresponds to the amino acid sequence <SEQ ID 2902; ORF 950. a>: a950.pep 1 MNKNIAAALA GALSLSLAAG AVAANKPASN ATGVHKSAHG SCGASKSAEG 51 SCGAAGSKAG EGKCGEGKCG ATVKKTHKHT KASKAKAKSA EGKCGEGKCG 101 SK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 950 shows 100.0% identity over a 102 aa overlap with a predicted ORF (ORF 950) from N meningitidis a950/ 950 100.0% identity in 102 aa overlap 10 20 30 40 50 60 a950.pep MΝKΝIAAALAGALSLSLAAGAVAAΝKPASΝATGVHKSAHGSCGASKSAEGSCGAAGSKAG I I II I I I I I I I I I I I I I II I I II II I II I I II I I I I I I I I I II I I I I I I II I II I I I I I I m950 MΝKΝIAAALAGALSLSLAAGAVAAΝKPASΝATGVHKSAHGSCGASKSAEGSCGAAGSKAG 10 20 30 40 50 60 70 80 90 100 a950.pep EGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSKX I I II I I I I I I I II I I I I M II I I I I I II I I II II I I I I I I I I m950 EGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK 70 80 90 100 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 2903>: g951.seq 1 ATGATTATGT TACCCGCCCG TTTCACTATT TTATCTGTCC TCGCAGCAGC 51 CCTGCTTGCC GGACAGGCGT ATGCTGCCGG CGCGGCGGAT GTGGAGCTGC 101 CGAAGGAAGT CGGAAAGGTT TTAAGGAAAC ATCGGCGTTA CAGCGAGGAA 151 GAAATCAAAA ACGAACGCGC ACGGCTTGCG GCAGTGGGCG AACGGGTCAA 201 CAGGGTGTTT ACGCTGTTGG GCGGTGAAAC GGCTTTGCAG AAAGGGCAGG 251 CGGGAACGGC TCTGGCAACC TATATGCTGA TGTTGGAACG CACAAAATCC 301 CCCGAAGTCG CCGAACGCGC CTTGGAAATG GCCGTGTCGC TGAACGCGTT 351 TGAACAGGCG GAAATGATTT ATCAGAAATG GCGGCAGATC GAGCCTATAC 401 CGGGTGAGGC GCAAAAACGG GCGGGGTGGC TGCGGAACGT ATTGAGGGAA 451 GGGGGAAATC AGCATCTGGA CGGGTTGGAA GAGGTGCTGG CGCAATCGGA 501 CGATGTGCAA AAACGCAGGA TATTTTTGCT GCTGGTGCAA GCCGCCGTGC 551 AGCAGGGTGG GGTGGCTCAA AAAGCATCGA AAGCGGTTCG CCGTGCGGCG 601 TTGAAGTATG AACATCTGCC CGAAGCGGCG GTTGCCGATG CGGTGTTCGG 651 CGTACAGGGA CGCGAAAAGG AAAAGGCAAT CGAAGCTTTG CAGCGTTTGG 701 CGAAGCTCGA TACGGAAATA TTGCCCCCCA CTTTAATGAC GTTGCGTCTG 751 ACTGCACGCA AATATCCCGA AATACTCGAC GGCTTTTTCG AGCAGACAGA 801 CACCCAAAAC CTTTCGGCCG TCTGGCAGGA AATGGAAATT ATGAATCTGG 851 TTTCCCTGCG TAAGCCGGAT GATGCCTATG CGCGTTTGAA CGTGCTGTTG 901 GAACACAACC CGAATGCAAA CCTGTATATT CAGGCGGCGA TATTGGCGGC 951 AAACCGAAAA GAAGGTGCGT CCGTTATCGA CGGCTACGCC GAAAAGGCAT 1001 ACGGCAGGGG GACGGGGGAA CAGCGGGGCA GGGCGGCAAT GACGGCGGCG 1051 ATGATATATG CCGACCGCAG GGATTACGCC AAAGTCAGGC AGTGGTTGAA 1101 AAAAGTGTCC GCGCCGGAAT ACCTGTTCGA CAAAGGCGTG CTGGCGGCTG 1151 CGGCGGCTGC CGAATTGGAC GGAGGCCGGG CGGCTTTGCG GCAGATCGGC 1201 AGGGTGCGGA AACTTCCCGA ACAGCAGGGG CGGTATTTTA CGGCAGACAA 1251 TTTGTCCAAA ATACAGATGC TCGCCCTGTC GAAGCTGCCC GACAAACGGG 1301 AAGCCCTGAT CGGGCTGAAC AACATCATCG CCAAACTTTC GGCGGCGGGA 1351 AGCACGGAAC CTTTGGCGGA AGCATTGGCA CAGCGTTCCA TTATTTACGA 1401 ACAGTTCGGC AAACGGGGAA AAATGATTGC CGACCTTGAA ACCGCGCTCA 1451 AACTTACGCC CGATAATGCA CAAATTATGA ATAATCTGGG CTACAGCCTG 1501 CTTTCCGATT CCAAACGTTT GGACGAGGGT TTCGCCCTGC TTCAGACGGC 1551 ATACCAAATC AACCCGGACG ATACCGCCGT TAACGACAGC ATAGGCTGGG 1601 CGTATTACCT GAAAGGCGAC GCGGAAAGCG CGCTGCCGTA TCTGCGGTAT 1651 TCGTTTGAAA ACGACCCCGA GCCCGAAGTT GCCGCCCATT TGGGCGAAGT 1701 GTTGTGGGCA TTGGGCGAAC GCGATCAGGC GGTTGACGTA TGGACGCAGG 1751 CGGCACACCT TAGGGGAGAC AAGAAAATAT GGCGGGAGAC GCTCAAACGC 1801 TACGGAATCG CCTTGCCCGA GCCTTCCCGA AAACCCCGGA AATAA This corresponds to the amino acid sequence <SEQ ID 2904; ORF 951.ng>: g951.pep 1 MIMLPARFTI LSVLAAALLA GQAYAAGAAD VELPKEVGKV LRKHRRYSEE 51 EIKNERARLA AVGERVNRVF TLLGGETALQ KGQAGTALAT YMLMLERTKS 101 PEVAERALEM AVSLNAFEQA EMIYQKWRQI EPIPGEAQKR AGWLRNVLRE 151 GGNQHLDGLE EVLAQSDDVQ KRRIFLLLVQ AAVQQGGVAQ KASKAVRRAA 201 KYEHLPEAA VADAVFGVQG REKEKAIEAL QRLAKLDTEI LPPTLMTLRL 251 TARKYPEILD GFFEQTDTQN LSAVWQEMEI MNLVSLRKPD DAYARLNVLL 301 EHNPNANLYI QAAILAANRK EGASVIDGYA EKAYGRGTGE QRGRAAMTAA 351 MIYADRRDYA KVRQWLKKVS APEYLFDKGV LAAAAAAELD GGRAALRQIG 401 RVRKLPEQQG RYFTADNLSK IQMLALSKLP DKREALIGLN NIIAKLSAAG 451 STEPLAEALA QRSIIYEQFG KRGKMIADLE TALKLTPDNA QIMNNLGYSL 501 LSDSKRLDEG FALLQTAYQI NPDDTAVNDS IGWAYYLKGD AEΞALPYLRY 551 SFENDPEPEV AAHLGEVLWA LGERDQAVDV WTQAAHLRGD KKIWRETLKR 601 YGIALPEPSR KPRK* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2905>: m951.seq 1 ATGATTATGT TACCTAACCG TTTCAAAATG TTAACTGTGT TGACGGCAAC 51 CTTGATTGCC GGACAGGTAT CTGCCGCCGG AGGCGGTGCG GGGGATATGA 101 AACAGCCGAA GGAAGTCGGA AAGGTTTTCA GAAAGCAGCA GCGTTACAGC 151 GAGGAAGAAA TCAAAAACGA ACGCGCACGG CTTGCGGCAG TGGGCGAGCG 201 GGTTAATCAG ATATTTACGT TGCTGGGAGG GGAAACCGCC TTGCAAAAGG 251 GGCAGGCGGG AACGGCTCTG GCAACCTATA TGCTGATGTT GGAACGCACA 301 AAATCCCCCG AAGTCGCCGA ACGCGCCTTG GAAATGGCCG TGTCGCTGAA 351 CGCGTTTGAA CAGGCGGAAA TGATTTATCA GAAATGGCGG CAGATTGAGC 401 CTATACCGGG TAAGGCGCAA AAACGGGCGG GGTGGCTGCG GAACGTGCTG 451 AGGGAAAGAG GAAATCAGCA TCTGGACGGA CTGGAAGAAG TGCTGGCTCA 501 GGCGGACGAA GGACAGAACC GCAGGGTGTT TTTATTGTTG GCACAAGCCG 551 CCGTGCAACA GGACGGGTTG GCGCAAAAAG CATCGAAAGC GGTTCGCCGC 601 GCGGCGTTGA AATATGAACA TCTGCCCGAA GCGGCGGTTG CCGATGTGGT 651 GTTCAGCGTA CAGGGACGCG AAAAGGAAAA GGCAATCGGA GCTTTGCAGC 701 GTTTGGCGAA GCTCGATACG GAAATATTGC CCCCCACTTT AATGACGTTG 751 CGTCTGACTG CACGCAAATA TCCCGAAATA CTCGACGGCT TTTTCGAGCA 801 GACAGACACC CAAAACCTTT CGGCCGTCTG GCAGGAAATG GAAATTATGA 851 ATCTGGTTTC CCTGCACAGG CTGGATGATG CCTATGCGCG TTTGAACGTG 901 CTGTTGGAAC GCAATCCGAA TGCAGACCTG TATATTCAGG CAGCGATATT 951 GGCGGCAAAC CGAAAAGAAG GTGCTTCCGT TATCGACGGC TACGCCGAAA 1001 AGGCATACGG CAGGGGGACG GAGGAACAGC GGAGCAGGGC GGCGCTAACG 1051 GCGGCGATGA TGTATGCCGA CCGCAGGGAT TACGCCAAAG TCAGGCAGTG 1101 GCTGAAAAAA GTATCCGCGC CGGAATACCT GTTCGACAAA GGTGTGCTGG 1151 CGGCTGCGGC GGCTGTCGAG TTGGACGGCG GCAGGGCGGC TTTGCGGCAG 1201 ATCGGCAGGG TGCGGAAACT TCCCGAACAG CAGGGGCGGT ATTTTACGGC 1251 AGACAATTTG TCCAAAATAC AGATGCTCGC CCTGTCGAAG CTGCCCGATA 1301 AACGGGAGGC TTTGAGGGGG TTGGACAAGA TTATCGAAAA ACCGCCTGCC 1351 GGCAGTAATA CAGAGTTACA GGCAGAGGCA TTGGTACAGC GGTCAGTTGT 1401 TTACGATCGG CTTGGCAAGC GGAAAAAAAT GATTTCAGAT CTTGAAAGGG 1451 CGTTCAGGCT TGCACCCGAT AACGCTCAGA TTATGAATAA TCTGGGCTAC 1501 AGCCTGCTGA CCGATTCCAA ACGTTTGGAC GAAGGTTTCG CCCTGCTTCA 1551 GACGGCATAC CAAATCAACC CGGACGATAC CGCTGTCAAC GACAGCATAG 1601 GCTGGGCGTA TTACCTGAAA GGCGACGCGG AAAGCGCGCT GCCGTATCTG 1651 CGGTATTCGT TTGAAAACGA CCCCGAGCCC GAAGTTGCCG CCCATTTGGG 1701 CGAAGTGTTG TGGGCATTGG GCGAACGCGA TCAGGCGGTT GACGTATGGA 1751 CGCAGGCGGC ACACCTTACG GGAGACAAGA AAATATGGCG GGAAACGCTC 1801 AAACGTCACG GCATCGCATT GCCCCAACCT TCCCGAAAAC CTCGGAAATA 1851 A This corresponds to the amino acid sequence <SEQ ID 2906; ORF 791>: m951.pep 1 MIMLPΝRFKM LTVLTATLIA GQVSAAGGGA GDMKQPKEVG KVFRKQQRYS 51 EEEIKΝERAR LAAVGERVΝQ IFTLLGGETA LQKGQAGTAL ATYMLMLERT 101 KSPEVAERAL EMAVSLΝAFE QAEMIYQKWR QIEPIPGKAQ KRAGWLRΝVL 151 RERGΝQHLDG LEEVLAQADE GQΝRRVFLLL AQAAVQQDGL AQKASKAVRR 201 AALKYEHLPE AAVADVVFSV QGREKEKAIG ALQRLAKLDT EILPPTLMTL 251 RLTARKYPEI LDGFFEQTDT QΝLSAVWQEM EIMΝLVSLHR LDDAYARLΝV 301 LLERΝPΝADL YIQAAILAAΝ RKEGASVIDG YAEKAYGRGT EEQRSRAALT 351 AAMMYADRRD YAKVRQWLKK VSAPEYLFDK GVLAAAAAVE LDGGRAALRQ 401 IGRVRKLPEQ QGRYFTADΝL SKIQMLALSK LPDKREALRG LDKIIEKPPA 451 GSΝTELQAEA LVQRSVVYDR LGKRKKMIΞD LERAFRLAPD ΝAQIMΝΝLGY 501 SLLTDSKRLD EGFALLQTAY QIΝPDDTAVΝ DSIGWAYYLK GDAESALPYL 551 RYSFEΝDPEP EVAAHLGEVL WALGERDQAV DVWTQAAHLT GDKKIWRETL 601 KRHGIALPQP SRKPRK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 951 shows 88.6% identity over a 616 aa overlap with a predicted ORF (ORF 951) from N gonorrhoeae 951/g951 88.6% identity m 616 aa overlap 10 20 30 40 50 60 m951.pep MIMLPΝRFKMLTVLTATLIAGQVSAAGGGAGDMKQPKEVGKVFRKQQRYSEEEIKΝERAR I I II I II : I M M M I : II M II IMM: II I I I I I : I I : : I I I II I I I II I I I g951 MIMLPARFTILSVLAAALLAGQAYAA--GAADVELPKEVGKVLRKHRRYSEEEIKΝERAR 10 20 30 40 50 70 80 90 100 110 120 m951.pep AAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPEVAERALEMAVSLNAFE I I II I I I I \ - - \ II II I I I I I I I I I I I I I I I I I I I I II I II I I I II I I I I I I I II I II I I g951 LAAVGERVNRVFTLLGGETALQKGQAGTALATYMLMLERTKSPEVAERALEMAVΞLNAFE 60 70 80 90 100 110 130 140 150 160 170 180 m951.pep QAEMIYQKWRQIEPIPGKAQKRAGWLRNVLRERGNQHLDGLEEVLAQADEGQNRRVFLLL I I I I I I I I I I I I I I II I : II I I I I I I I I I I I I I I I I I I I I I I I I I I : I : I : I I : I I II g951 QAEMIYQKWRQIEPIPGEAQKRAGWLRNVLREGGNQHLDGLEEVLAQSDDVQKRRIFLLL 120 130 140 150 160 170 190 200 210 220 230 240 m951. pep AQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSVQGREKEKAIGALQRLAKLDT MIIIM I : I I II II I I II I I I I II I I I II I I I I : I I : I I I I I I I I I I I I I I I I M I I g951 VQAAVQQGGVAQKASKAVRRAALKYEHLPEAAVADAVFGVQGREKEKAIEALQRLAKLDT 180 190 200 210 220 230 250 260 270 280 290 300 m951.pep EILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEMEIMNLVSLHRLDDAYARLNV I I I I I I I I I I I I I I I I I I I I I II II I I I I I II I II I I I I II I II I I I I :: lll g951 EILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEMEIMNLVSLRKPDDAYARLNV 240 250 260 270 280 290 310 320 330 340 350 360 m951. pep LLERNPNADLYIQAAILAANRKEGASVIDGYAEKAYGRGTEEQRSRAALTAAMMYADRRD I I I : I I I I : I I I I I II I I I I I II I I I I I I I I I I I I I II I I I I I : II M I I I I : I I I I I I g951 LLEHNPNANLYIQAAILAANRKEGASVIDGYAEKAYGRGTGEQRGRAAMTAAMIYADRRD 300 310 320 330 340 350 370 380 390 400 410 420 m951. pep YAKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGGRAALRQIGRVRKLPEQQGRYFTADNL I I II I I II I I I I I I I II II I II I II I I Ml II I I I I I I I II I II I I I I I I I I I II I I I I I g951 YAKVRQWLKKVSAPEYLFDKGVLAAAAAAELDGGRAALRQIGRVRKLPEQQGRYFTADNL 360 370 380 390 400 410 430 440 450 460 470 480 m951.pep SKIQMLALSKLPDKREALRGLDKIIEKPPAGSNTELQAEALVQRSVVYDRLGKRKKMISD I I I I I I I I I I I I II I I I I I M : I I I I ::: I I i I I I : I I I : : M : : I I I IMM g951 SKIQMLALSKLPDKREALIGLNNIIAKLSAAGSTEPLAEALAQRSIIYEQFGKRGKMIAD 420 430 440 450 460 470 490 500 510 520 530 540 m951.pep LERAFRLAPDNAQIMNNLGYSLLTDΞKRLDEGFALLQTAYQINPDDTAVNDSIGWAYYLK II MM : I I II I I I II I I I I I I : I I I II I II I I I I I I I II I I I I I I I I I I I I I I I I I I I g951 LETALKLTPDNAQIMNNLGYSLLSDSKRLDEGFALLQTAYQINPDDTAVNDSIGWAYYLK 480 490 500 510 520 530 550 560 570 580 590 600 m951. pep GDAESALPYLRYSFENDPEPEVAAHLGEVLWALGERDQAVDVWTQAAHLTGDKKIWRETL I I I I I II I I I I I II I I I I I I I II I I I I II I I I I I I I II I I I I I I I I I I I llllllllll g951 GDAESALPYLRYSFENDPEPEVAAHLGEVLWALGERDQAVDVWTQAAHLRGDKKIWRETL 540 550 560 570 580 590 610 m951.pep KRHGIALPQPSRKPRK I I : II I I I : I I I I I I I g951 KRYGIALPEPSRKPRKX 600 610 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2907>: a951. seq 1 ATGTTACCCG CCCGTTTCAC CATTTTATCT GTGCTCGCGG CAGCCCTGCT 51 TGCCGGGCAG GCGTATGCCG CCGGCGCGGC GGATGCGAAG CCGCCGAAGG 101 AAGTCGGAAA GGTTTTCAGA AAGCAGCAGC GTTACAGCGA GGAAGAAATC 151 AAAAACGAAC GCGCACGGCT TGCGGCAGTG GGCGAGCGGG TTAATCAGAT 201 ATTTACGTTG CTGGGAGGGG AAACCGCCTT GCAAAAGGGG CAGGCGGGAA 251 CGGCTCTGGC AACCTATATG CTGATGTTGG AACGCACAAA ATCCCCCGAA 301 GTCGCCGAAC GCGCCTTGGA AATGGCCGTG TCGCTGAACG CGTTTGAACA 351 GGCGGAAATG ATTTATCAGA AATGGCGGCA GATTGAGCCT ATACCGGGTA 401 AGGCGCAAAA ACGGGCGGGG TGGCTGCGGA ACGTGCTGAG GGAAAGAGGA 451 AATCAGCATC TAGACGGACT GGAAGAAGTG CTGGCTCAGG CGGACGAAGG 501 ACAGAACCGC AGGGTGTTTT TATTGTTGGC ACAAGCCGCC GTGCAACAGG 551 ACGGGTTGGC GCAAAAAGCA TCGAAAGCGG TTCGCCGCGC GGCGTTGAGA 601 TATGAACATC TGCCCGAAGC GGCGGTTGCC GATGTGGTGT TCAGCGTACA 651 GGGACGCGAA AAGGAAAAGG CAATCGGAGC TTTGCAGCGT TTGGCGAAGC 701 TCGATACGGA AATATTGCCC CCCACTTTAA TGACGTTGCG TCTGACTGCA 751 CGCAAATATC CCGAAATACT CGACGGCTTT TTCGAGCAGA CAGACACCCA 801 AAACCTTTCG GCCGTCTGGC AGGAAATGGA AATTATGAAT CTGGTTTCCC 851 TGCACAGGCT GGATGATGCC TATGCGCGTT TGAACGTGCT GTTGGAACGC 901 AATCCGAATG CAGACCTGTA TATTCAGGCA GCGATATTGG CGGCAAACCG 951 AAAAGAAGGT GCTTCCGTTA TCGACGGCTA CGCCGAAAAG GCATACGGCA 1001 GGGGGACGGG GGAACAGCGG GGCAGGGCGG CAATGACGGC GGCGATGATA 1051 TATGCCGACC GAAGGGATTA CACCAAAGTC AGGCAGTGGT TGAAAAAAGT 1101 GTCCGCGCCG GAATACCTGT TCGACAAAGG TGTGCTGGCG GCTGCGGCGG 1151 CTGTCGAGTT GGACGGCGGC AGGGCGGCTT TGCGGCAGAT CGGCAGGGTG 1201 CGGAAACTTC CCGAACAGCA GGGGCGGTAT TTTACGGCAG ACAATTTGTC 1251 CAAAATACAG ATGTTCGCCC TGTCGAAGCT GCCCGACAAA CGGGAGGCTT 1301 TGAGGGGGTT GGACAAGATT ATCGAAAAAC CGCCTGCCGG CAGTAATACA 1351 GAGTTACAGG CAGAGGCATT GGTACAGCGG TCAGTTGTTT ACGATCGGCT 1401 TGGCAAGCGG AAAAAAATGA TTTCAGATCT TGAAAGGGCG TTCAGGCTTG 1451 CACCCGATAA CGCTCAGATT ATGAATAATC TGGGCTACAG CCTGCTTTCC 1501 GATTCCAAAC GTTTGGACGA AGGCTTCGCC CTGCTTCAGA CGGCATACCA 1551 AATCAACCCG GACGATACCG CTGTCAACGA CAGCATAGGC TGGGCGTATT 1601 ACCTGAAAGG CGACGCGGAA AGCGCGCTGC CGTATCTGCG GTATTCGTTT 1651 GAAAACGACC CCGAGCCCGA AGTTGCCGCC CATTTGGGCG AAGTGTTGTG 1701 GGCATTGGGC GAACGCGATC AGGCGGTTGA CGTATGGACG CAGGCGGCAC 1751 ACCTTACGGG AGACAAGAAA ATATGGCGGG AAACGCTCAA ACGTCACGGC 1801 ATCGCATTGC CCCAACCTTC CCGAAAACCT CGGAAATAA This corresponds to the amino acid sequence <SEQ ID 2908; ORF 951.a>: a951.pep 1 MLPARFTILS VLAAALLAGQ AYAAGAADAK PPKEVGKVFR KQQRYSEEEI 51 KΝERARLAAV GERVΝQIFTL LGGETALQKG QAGTALATYM LMLERTKSPE 101 VAERALEMAV SLΝAFEQAEM IYQKWRQIEP IPGKAQKRAG WLRΝVLRERG 151 ΝQHLDGLEEV LAQADEGQΝR RVFLLLAQAA VQQDGLAQKA SKAVRRAALR 201 YEHLPEAAVA DVVFSVQGRE KEKAIGALQR LAKLDTEILP PTLMTLRLTA 251 RKYPEILDGF FEQTDTQΝLΞ AVWQEMEIMΝ LVSLHRLDDA YARLΝVLLER 301 ΝPΝADLYIQA AILAAΝRKEG ASVIDGYAEK AYGRGTGEQR GRAAMTAAMI 351 YADRRDYTKV RQWLKKVSAP EYLFDKGVLA AAAAVELDGG RAALRQIGRV 401 RKLPEQQGRY FTADΝLSKIQ MFALSKLPDK REALRGLDKI IEKPPAGSΝT 451 ELQAEALVQR SVVYDRLGKR KKMISDLERA FRLAPDΝAQI MΝΝLGYSLLS 501 DSKRLDEGFA LLQTAYQIΝP DDTAVΝDSIG WAYYLKGDAE SALPYLRYSF 551 EΝDPEPEVAA HLGEVLWALG ERDQAVDVWT QAAHLTGDKK IWRETLKRHG 600 IALPQPSRKP RK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 951 shows 96.4% identity over a 614 aa overlap with a predicted ORF (ORF 951) from N meningitidis a951/ 951 96.4% identity in 614 aa overlap 10 20 30 40 50 a951.pep MLPARFTILSVLAAALLAGQAYAAG—AADAKPPKEVGKVFRKQQRYSEEEIKΝERAR III II M M MMIM I M III IM I I I I I I I I I I I I I I I I II I M I I I m951 MIMLPΝRFKMLTVLTATLIAG Q VSAAGGGAGDMKQPKEVGKVFRKQQRYSEEEIKΝERAR 10 20 30 40 50 60 60 70 80 90 100 110 a951. pep LAAVGERVΝQIFTLLGGETAL Q KG Q AGTALATYMLMLERTKSPEVAERALEMAVSLΝAFE I I II I I I II II II II I II I II I I I I I I I I I I I I I I I I I I I I I II I I I I II I I I I I I I I I I m951 LAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPEVAERALEMAVSLNAFE 70 80 90 100 110 120 120 130 140 150 160 170 a951.pep QAEMIYQKWRQIEPIPGKAQKRAGWLRNVLRERGNQHLDGLEEVLAQADEGQNRRVFLLL I I II I I I I I I I I I I I I I I I I I I I II I II II I I I I I II I I I I I I I I I I I I I I I I I I I I I I I m951 QAEMIYQKWRQIEPIPGKAQKRAGWLRNVLRERGNQHLDGLEEVLAQADEGQNRRVFLLL 130 140 150 160 170 180 180 190 200 210 220 230 a951.pep AQAAVQQDGLAQKASKAVRRAALRYEHLPEAAVADVVFSVQGREKEKAIGALQRLAKLDT I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I II I I I I I II I I I I I I I I I I 951 AQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSVQGREKEKAIGALQRLAKLDT 190 200 210 220 230 240 240 250 260 270 280 290 a951.pep EILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEMEIMNLVSLHRLDDAYARLNV I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m951 EILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEMEIMNLVSLHRLDDAYARLNV 250 260 270 280 290 300 300 310 320 330 340 350 a951.pep LLERNPNADLYIQAAILAANRKEGASVIDGYAEKAYGRGTGEQRGRAAMTAAMIYADRRD I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I M I I I : I I I I I I II 1 951 LLERNPNADLYIQAAILAANRKEGASVIDGYAEKAYGRGTEEQRSRAALTAAMMYADRRD 310 320 330 340 350 360 360 370 380 390 400 410 a951.pep YTKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGGRAALRQIGRVRKLPEQQGRYFTADNL I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I II I I I I I I I I I II I m951 YAKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGGRAALRQIGRVRKLPEQQGRYFTADNL 370 380 390 400 410 420 420 430 440 450 460 470 a951.pep SKIQMFALSKLPDKREALRGLDKIIEKPPAGSNTELQAEALVQRSVVYDRLGKRKKMISD I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I II I II I I m951 SKIQMLALSKLPDKREALRGLDKIIEKPPAGSNTELQAEALVQRSVVYDRLGKRKKMISD 430 440 450 460 470 480 480 490 500 510 520 530 a951.pep LERAFRLAPDNAQIMNNLGYSLLSDSKRLDEGFALLQTAYQINPDDTAVNDSIGWAYYLK I I II II II I I I I I I II I I I I I I M I I I I I I I I I I I II I I I I I I II I I I I II I I I I I I I I I m951 LERAFRLAPDNAQIMNNLGYSLLTDSKRLDEGFALLQTAYQINPDDTAVNDSIGWAYYLK 490 500 510 520 530 540 540 550 560 570 580 590 a951.pep GDAESALPYLRYSFENDPEPEVAAHLGEVLWALGERDQAVDVWTQAAHLTGDKKIWRETL I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I m951 GDAES LPYLRYSFENDPEPEVAAHLGEVLWALGERDQAVDVWTQAAHLTGDKKIWRETL 550 560 570 580 590 600 600 610 a951.pep KRHGIALPQPSRKPRK I I I I I I I I I I I I I I II m951 KRHGIALPQPSRKPRK 610 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2909>: g952. .seq (partial) 1 ..TTGTCTTATC GTTTGAATGC TGCACCGATG TTTAACGATA ATCCTGTTGT 51 TTACGGAAAA ATCAAATTGC AGAGTTGGAA AGCGCGGCGG GATTTCAATA 101 TTGTAAAGCA GGATTTGGAT TTTTCCTGCG GGGCGGCTTC GGTGGCGACG 151 CTTTTGAACA ATTTTTACGG GCAAAAGCTG ACGGAAGAAG AAGTGTTGGA 201 AAAACTGGGT AAGGAACAGA TGCGCGCGTC GTTTGAGGAT ATGCGGCGCA 251 TTATGCCCGA TTTGGGTTTT GAGGCGAAAG GCTATGCCCT GTCTTTCGAA 301 CAGCTCGCGC AGTTGAAAAT CCCCGTCATC GTGTATCTGA AATACCGCAA 351 AGACGACCAT TTTTCGGTAT TGCGCGGAGT GGATGGCAAT ACGGTTTTGC 401 TTGCCGACCC GTCGCCGGGT CATGTTTCGA TGAGCAGGGC GCAGTTTTTG 451 GAGGCTTGGC AAACCCGTGA GGGAAATTTG GCAGGCAAAA TTTTGGCGGT 501 CGTGCCGAAA AAAGCGGAGG CGATTTCAAA TAAATTGTTT TTCACACATC 551 ATCCCAAGCG GCAGACGGAG TTTGCAGTCG GACAGGTAAA ATGGTGGCGT 601 GCTTATTGA This corresponds to the amino acid sequence <SEQ ID 2910; ORF 952.ng>: g952.pep (partial) 1 ..LSYRLNAAPM FNDNPVVYGK IKLQSWKARR DFNIVKQDLD FSCGAASVAT 51 LLNNFYGQKL TEEEVLEKLG KEQMRASFED MRRIMPDLGF EAKGYALSFE 101 QLAQLKIPVI VYLKYRKDDH FSVLRGVDGN TVLLADPSPG HVSMSRAQFL 151 EAWQTREGNL AGKILAVVPK KAEAISNKLF FTHHPKRQTE FAVGQVKWWR 201 AY* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2911>: 952.seq 1 ATGATGAAGT TCAAATATGT TTTTCTGTTG GCGTGTGTTG TCGTTTCTTT 51 ATCTTATCGT TTGAATGCTG CACCGATGTT TAACGATAAT CCTGTTGTTT 101 ACGGAAAAAT CAAAGTGCAG AGTTGGAAAG CGCGGCGGGA TTTCAATATT 151 GTAAAGCAGG ATTTGGATTT TTCCTGTGGG GCGGCTTCGG TGGCGACGCT 201 TTTGAACAAT TTTTACGGGC AAACGCTGAC GGAAGAAGAA GTGTTGAAAA 251 AGCTGGATAA GGAGCAGATG CGCGCGTCGT TTGAGGATAT GCGGCGCATT 301 ATGCCTGATT TGGGTTTTGA GGCGAAGGGC TATGCCCTGT CTTTCGAGCA 351 GCTCGCGCAG TTGAAAATCC CCGTCATCGT GTATCTGAAA TACCGCAAAG 401 ACGACCATTT TTCGGTATTG CGCGGTATAG ACGGCAATAC GGTTTTGCTT 451 GCCGACCCGT CGCTGGGGCA TGTTTCAATG AGCAGGGCGC AGTTTTTGGA 501 TGCTTGGCAA ACCCGTGAGG GAAATTTGGC AGGTAAGATT TTGGCTGTCA 551 TACCGAAAAA AGCCGAGACA ATTTCAAATA AATTGTTTTT CACACAACAC 601 CCAAAACGGC AGACGGAGTT TACAGTCGGA CAAATCAGGC AAGCACGTGC 651 AGAGTAA This corresponds to the amino acid sequence <SEQ ID 2912; ORF 952>: m952.pep 1 MMKFKYVFLL ACVVVSLSYR LΝAAPMFΝDΝ PVVYGKIKVQ SWKARRDFΝI 51 VKQDLDFSCG AASVATLLΝΝ FYGQTLTEEE VLKKLDKEQM RASFEDMRRI 101 MPDLGFEAKG YALSFEQLAQ LKIPVIVYLK YRKDDHFSVL RGIDGΝTVLL 151 ADPSLGHVSM SRAQFLDAWQ TREGΝLAGKI LAVIPKKAET ISΝKLFFTQH 201 PKRQTEFTVG QIRQARAE* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 952 shows 92.5% identity over a 201 aa overlap with a predicted ORF (ORF 952) from N gonorrhoeae g952/m952; 92.5% identity in 201 aa overlap 10 20 30 40 g952.pep LSYRLΝAAPMFΝDΝPVVYGKIKLQSWKARRDFΝIVKQDLDFSCG I I I I I I I I I I I I I I I I I I I I I I M I I I I I I I I I I I I I I I I I I I i m952 MMKFKYVFLLACVVVSLSYRLΝAAPMFΝDΝPVVYGKIKVQSWKARRDFΝIVKQDLDFSCG 10 20 30 40 50 60 50 60 70 80 90 100 g952. pep AASVATLLΝΝFYGQKLTEEEVLEKLGKEQMRASFEDMRRIMPDLGFEAKGYALSFEQLAQ llllllllllllll I I I I I II : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I m952 AASVATLLΝΝFYGQTLTEEEVLKKLDKEQMRASFEDMRRIMPDLGFEAKGYALSFEQLAQ 70 80 90 100 110 120 110 120 130 140 150 160 g952.pep LKIPVIVYLKYRKDDHFSVLRGVDGΝTVLLADPSPGHVSMSRAQFLEAWQTREGΝLAGKI I I II I I I I I I I I I I I II I I I I M I I I II I I I I M I I I I I I I I I I I M I I I I I I I I I I M m952 LKIPVIVYLKYRKDDHFSVLRGIDGΝTVLLADPSLGHVSMSRAQFLDAWQTREGΝLAGKI 130 140 150 160 170 180 170 180 190 200 g952.pep LAVVPKKAEAISΝKLFFTHHPKRQTEFAVGQVKWWRAYX I I I M I I I M I I I I I I I I : I I I I II I I : I I I : : II m952 LAVIPKKAETISΝKLFFTQHPKRQTEFTVGQIRQARAE 190 200 210 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2913>: a952.seq 1 ATGATGAAGT TCAAATATGT TTTTCTGTTG GCGTGTGTTG TCGTTTCTTT 51 ATCTTATCGT TTGAATGCTG CACCGATGTT TAACGATAAT CCTGTTGTTT 101 ACGGAAAAAT CAAAGTGCAG AGTTGGAAAG AAAGGCGGGA TTTCAATATT 151 GTAAAGCAGG ATTTGGATTT TTCCTGCGGG GCGGCTTCGG TGGCGACGCT 201 TTTGAACAAT TTTTACGGGC AAACGCTGAC GGAAGAAGAA GTGTTGAAAA 251 AGCTGGATAA GGAGCAGATG CGCGCGTCGT TTGAGGATAT GCGGCGCATT 301 ATGCCAGATT TGGGTTTTGA AGCGAAAGGC TATGCCCTGT CTTTCGAGCA 351 GCTCGCACAG TTGAAAATCC CCGTCATCGT GTATCTGAAA TACCGCAAGG 401 ATGATCATTT CTCGGTATTG CGCGGGATAG ACGGCAATAC GGTTTTGCTT 451 GCCGACCCGT CGCTGGGTCA TGTTTCAATG AGCAGGGCGC AGTTTTNGGA 501 TGCTTGGCAA ACCCGTGAGG GAAATTTGGC AGGTAAGATT TTGGCGGTCG 551 TGCCGAAAAA AGCCGAGACA ATTTCAAATA AATTGTTTTT CACACATCAT 601 CCCAAGCGGC AGACGGAGTT TGCAGTCGGA CAAATCAGGC AAGCACGTGC 651 AGAGTAA This corresponds to the amino acid sequence <SEQ ID 2914; ORF 952. a>: a952.peρ 1 MMKFKYVFLL ACVVVSLSYR LNAAPMFNDN PVVYGKIKVQ SWKERRDFNI 51 VKQDLDFSCG AASVATLLNN FYGQTLTEEE VLKKLDKEQM RASFEDMRRI 101 MPDLGFEAKG YALSFEQLAQ LKIPVIVYLK YRKDDHFSVL RGIDGNTVLL 151 ADPSLGHVSM SRAQFXDAWQ TREGNLAGKI LAVVPKKAET ISNKLFFTHH 201 PKRQTEFAVG QIRQARAE* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N. meningitidis ORF 952 shows 97.7% identity over a 218 aa overlap with a predicted ORF (ORF 952) from N meningitidis a952/m952 97.7% identity in 218 aa overlap 10 20 30 40 50 60 a952.pep MMKFKYVFLLACVVVSLSYRLΝAAPMFΝDΝPVVYGKIKVQSWKERRDFΝIVKQDLDFSCG I I I II I II I I I I I I I I I I I I I I II I I I! II I I I 1 I I I I II I I I I I I I I I I I II I I I I I I m952 MMKFKYVFLLACVVVSLSYRLΝAAPMFΝDΝPVVYGKIKVQSWKARRDFΝIVKQDLDFSCG 10 20 30 40 50 60 70 80 90 100 110 120 a952. pep AASVATLLΝΝFYGQTLTEEEVLKKLDKEQMRASFEDMRRIMPDLGFEAKGYALSFEQLAQ I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m952 AASVATLLΝΝFYGQTLTEEEVLKKLDKEQMRASFEDMRRIMPDLGFEAKGYALSFEQLAQ 70 80 90 100 110 120 130 140 150 160 170 180 a952.pep LKIPVIVYLKYRKDDHFSVLRGIDGΝTVLLADPSLGHVSMSRAQFXDAWQTREGΝLAGKI I I I II I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I llllllllllllll m952 KIPVIVYLKYRKDDHFSVLRGIDGΝTVLLADPSLGHVSMSRAQFLDAWQTREGΝLAGKI 130 140 150 160 170 180 190 200 210 219 a952.pep LAVVPKKAETISΝKLFFTHHPKRQTEFAVGQIRQARAEX I I I : I I II I I I I II I II I : I I I I I I I I : II I I I I I I I I m952 LAVIPKKAETISΝKLFFTQHPKRQTEFTVGQIRQARAE 190 200 210 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 2915>: g953.seq 1 ATGAAAAAAA TCATCTTCGC CGCGCTCGCA GCGGCAGCCG TCGGCACTGC 51 CTCCGCCACC TACAAAGTGG ACGAATATCA CGCCAACGTC CGTTTCGCCA 101 TCGACCACTT CAACACCAGC ACCAACGTCG GCGGTTTTTA CGGTCTGACC 151 GGTTCCGTCG AGTTCGATCA AGCAAAACGC GACGGCAAAA TCGACATCAC 201 CATTCCCGTC GCCAACCTGC AAAGCGGTTC GCAACCCTTC ACCGGCCACC 251 TGAAATCCGC CGACATCTTC GATGCCGCTC AATATCCGGA CATCCGCTTC 301 GTTTCCACCA AATTCAACTT CAACGGCAAA AAACTTGTTT CCGTTGACGG 351 CAACCTGACC ATGCGCGGCA AAACCGCCCC CGTCAAACTC AAAGCCGAAA 401 AATTCAACTG CTACCAAAGC CCGATGGCGG AAACCGAAGT TTGCGGCGGC 451 GACTTCAGCA CCACCATCGA CCGCACCAAA TGGGGCGTGG ACTACCTCGT 501 TAACGCCGGT ATGACCAAAA ACGTCCGCAT CGACATCCAA ATCGAAGCTG 551 CAAAACAATA A This corresponds to the amino acid sequence <SEQ ID 2916; ORF 953.ng>: g953.pep 1 MKKIIFAALA AAAVGTASAT YKVDEYHANV RFAIDHFNTS TNVGGFYGLT 51 GSVEFDQAKR DGKIDITIPV ANLQSGSQPF TGHLKSADIF DAAQYPDIRF 101 VSTKFNFNGK KLVSVDGNLT MRGKTAPVKL KAEKFNCYQS PMAETEVCGG 151 DFSTTIDRTK WGVDYLVNAG MTKNVRIDIQ IEAAKQ* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2917>: m953.seq 1 ATGAAAAAAA TCATCTTCGC CGCACTCGCA GCCGCCGCCA TCAGTACTGC 51 CTCCGCCGCC ACCTACAAAG TGGACGAATA TCACGCCAAC GCCCGTTTCG 101 CCATCGACCA TTTCAACACC AGCACCAACG TCGGCGGTTT TTACGGTCTG 151 ACCGGTTCCG TCGAGTTCGA CCAAGCAAAA CGCGACGGTA AAATCGACAT 201 CACCATCCCC ATTGCCAACC TGCAAAGCGG TTCGCAACAC TTTACCGACC 251 ACCTGAAATC AGCCGACATC TTCGATGCCG CCCAATATCC GGACATCCGC 301 TTTGTTTCCA CCAAATTCAA CTTCAACGGC AAAAAACTGG TTTCCGTTGA 351 CGGCAACCTG ACCATGCACG GCAAAACCGC CCCCGTCAAA CTCAAAGCCG 401 AAAAATTCAA CTGCTACCAA AGCCCGATGG AGAAAACCGA AGTTTGTGGC 451 GGCGACTTCA GCACCACCAT CGACCGCACC AAATGGGGCA TGGACTACCT 501 CGTTAACGTT GGTATGACCA AAAGCGTCCG CATCGACATC CAAATCGAGG 551 CAGCCAAACA ATAA This corresponds to the amino acid sequence <SEQ ID 2918; ORF 953>: m953.pep 1 MKKIIFAALA AAAISTASAA TYKVDEYHAΝ ARFAIDHFΝT STΝVGGFYGL 51 TGSVEFDQAK RDGKIDITIP IAΝLQSGSQH FTDHLKSADI FDAAQYPDIR 101 FVSTKFΝFΝG KKLVSVDGΝL TMHGKTAPVK LKAEKFΝCYQ SPMEKTEVCG 151 GDFSTTIDRT KWGMDYLVΝV GMTKSVRIDI QIEAAKQ* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N. gonorrhoeae ORF 953 shows 93.0% identity over a 187 aa overlap with a predicted ORF (ORF 953) from N gonorrhoeae m953/g953 93.0% identity in 187 aa overlap 10 20 30 40 50 60 Itl953 . pep MKKI IFAALAAAAISTASAATYKVDEYHAΝARFAIDHFΝTSTΝVGGFYGLTGSVEFDQAK I I I I I I I I I I I I M M I I I I I I I I I I I I I M I I I I I I I I I I I I I I I I I I I I I I I I I I I I g953 MKKI IFAALAAAAVGTASA-TYKVDEYHAΝVRFAIDHFΝTSTΝVGGFYGLTGΞVEFDQAK 10 20 30 40 50 70 80 90 100 110 120 m953.pep RDGKIDITIPIAΝLQSGSQHFTDHLKSADIFDAAQYPDIRFVSTKFΝFΝGKKLVSVDGΝL I I I I I I II II M I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I g953 RDGKIDITIPVAΝLQSGSQPFTGHLKSADIFDAAQYPDIRFVSTKFΝFΝGKKLVSVDGΝL 60 70 80 90 100 110 130 140 150 160 170 180 m953. pep TMHGKTAPVKLKAEKFΝCYQSPMEKTEVCGGDFSTTIDRTKWGMDYLVΝVGMTKSVRIDI II : I I I II I I II I II II I I I I I I M I I I I I I I I I I I I I I I I I M I I I I M I I M I I I I I g953 TMRGKTAPVKLKAEKFΝCYQSPMAETEVCGGDFSTTIDRTKWGVDYLVΝAGMTKΝVRIDI 120 130 140 150 160 170 m953.pep QIEAAKQX llllllll g953 QIEAAKQX 180 The following partial DΝA sequence was identified in N. . meningitidis <SEQ ID 2919>: a953. seq 1 ATGAAAAAAA TCATCATCGC CGCGCTCGCA GCAGCCGCCA TCGGCACTGC 51 CTCCGCCGCC ACCTACAAAG TGGACGAATA TCACGCCAAC GCCCGTTTCT 101 CTATCGACCA TTTCAACACC AGCACCAACG TCGGCGGTTT TTACGGTCTG 151 ACCGGTTCCG TTGAGTTCGA CCAAGCAAAA CGCGACGGTA AAATCGACAT 201 CACCATCCCC GTTGCCAACC TGCAAAGCGG TTCGCAACAC TTTACCGACC 251 ACCTGAAATC AGCCGACATC TTCGATGCCG CCCAATATCC GGACATCCGC 301 TTTGTTTCCA CCAAATTCAA CTTCAACGGC AAAAAACTGG TTTCCGTTGA 351 CGGCAACCTG ACCATGCACG GCAAAACCGC CCCCGTCAAA CTCAAAGCCG 401 AAAAATTCAA CTGCTACCAA AGCCCGATGT TGAAAACCGA AGTTTGCGGC 451 GGCGACTTCA GCACCACCAT CGACCGCACC AAATGGGGCA TGGACTACCT 501 CGTTAACGTT GGTATGACCA AAAGCGTCCG CATCGACATC CAAATCGAGG 551 CAGCCAAACA ATAA This corresponds to the amino acid sequence <SEQ ID 2920; ORF 953. a>: a953.pep 1 MKKIIIAALA AAAIGTASAA TYKVDEYHAN ARFSIDHFNT STNVGGFYGL 51 TGSVEFDQAK RDGKIDITIP VANLQSGSQH FTDHLKSADI FDAAQYPDIR 101 FVSTKFNFNG KKLVSVDGNL TMHGKTAPVK LKAEKFNCYQ SPMLKTEVCG 151 GDFSTTIDRT KWGMDYLVNV GMTKSVRIDI QIEAAKQ* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 953 shows 97.3% identity over a 187 aa overlap with a predicted ORF (ORF 953) from N meningitidis a953/m953 97.3% identity in 187 aa overlap 10 20 30 40 50 60 a953.pep MKKIIIAALAAAAIGTASAATYKVDEYHAΝARFSIDHFΝTSTΝVGGFYGLTGSVEFDQAK I I I I I M I I I I I I I M I I I I I I I I I I I I I I I I I M II I I I I I I I I M I I I I I I I I II M I m953 MKKIIFAALAAAAISTASAATYKVDEYHAΝARFAIDHFΝTSTΝVGGFYGLTGSVEFDQAK 10 20 30 40 50 60 70 80 90 100 110 120 a953.pep RDGKIDITIPVAΝLQSGSQHFTDHLKSADIFDAAQYPDIRFVSTKFΝFΝGKKLVSVDGΝL I I II I I I I I I : I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I m953 RDGKIDITIPIAΝLQSGSQHFTDHLKSADIFDAAQYPDIRFVSTKFΝFΝGKKLVSVDGΝL 70 80 90 100 110 120 130 140 150 160 170 180 a953.pep TMHGKTAPVKLKAEKFΝCYQSPMLKTEVCGGDFSTTIDRTKWGMDYLVΝVGMTKSVRIDI I I II II I I I I I II I I I I I I I II I I I II I I I I I I I I I I I I I I I I I M I I I I I I I I I I I I I m953 TMHGKTAPVKLKAEKFΝCYQSPMEKTEVCGGDFSTTIDRTKWGMDYLVΝVGMTKSVRIDI 130 140 150 160 170 180 a953.pep QIEAAKQX llllllll m953 QIEAAKQX g954. seq not found yet g954.pep not found yet The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2921>: m954.seq 1 ATGAAAAAGT TTTATTTTGT GCTGCTGGCG TTGGGTTTGG CAGCGTGTGG 51 GCAAGAACAA TCGCAGAAAG CTGATGCGGA GCAGTATTTT TTTGCCAATA 101 AATATCAATT TGCAGATGAG AAACAGGCTT TTTATTTTGA ACGCGCCGCC 151 CGTTTCCGTG TATTGCAACA AGGCCTTGGC GGGGATTTTG AGAGGTTTTT 201 AAAAGGAGAA ATACCTAATC AAGAAAATCT TGCAAAGTAT CGTGAAAATA 251 TTACTCAAGC AGTCGCTTAT TATGCGGACA CGAATGGAGA TGATGACCCA 301 TACCGCGTCT GCAAACAGGC TGCGCAAGAT GCAGAAATCC TGATGAAGAG 351 TATGGTAACA AGCGGTGGAG GCGGTACAAC TGATTTAGAT AAGGAAAGTT 401 ATCAAAATTA CCGAAAATCA ATGCAAGAAT GCCGTAAAAC AATAACGGAA 451 GCTGAAGCCA ATTTGCCGAA AAAATAA This corresponds to the amino acid sequence <SEQ ID 2922; ORF 954>: m95 .pep 1 MKKFYFVLLA LGLAACGQEQ ΞQKADAEQYF FAΝKYQFADE KQAFYFERAA 51 RFRVLQQGLG GDFERFLKGE IPΝQEΝLAKY REΝITQAVAY YADTΝGDDDP 101 YRVCKQAAQD AEILMKSMVT SGGGGTTDLD KESYQΝYRKS MQECRKTITE 151 AEAΝLPKK* a954. seq not found yet a954.pep not found yet The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2923>: g957. seq (partial) 1 ATGTTTAAAA AATTCAAACC GGTACTGTTG TCATTTTTTG CACTTGTATT 51 TGCCTTTTGG CTGGGAACAG GTATTGCCTA TGAGATTAAT CCGCGTTGGT 101 TTTTGAGCGA TACGGCAACT GAAGTACCTG AAAATCCGAA TGCTTTTGTG 151 GCGAAACTTG CCCGCCTGTT CCGAAATGCC GACAGGGCGG TTGTCATCGT 201 GAAGGAATCG ATGAGGACGG AGGAAAGCCT TGCCGGAGCT GTGGATGACG 251 GTCCGTTGCA GTCGGAGAAG GATTATCTCG CGCTCGCTAT CCGGCTCAGT 301 CGTTTGAAAG AAAAGGCGAA ATGGTTTCAC GTAACGGAGC AGGAACATGG 351 GGAAGAGGTT TGGCTGGATT ACTATATCGG CGAGGGCGGT TTGGTTGCGG 401 TTTCGCTTTC GCAACGCTCG CCGGAAGCGT TTGTTAATGC CGAATATCTG 451 TATCGGAACG ATCGTCCGTT TTCTGTAAAT GTGTACGGCG GAACGGCTCA 501 CGGGGAAAAT TATGAAACGA CAGGAGAATA TCGGGTTGTT TGGCAACCGG 551 ACGGTTCGGT ATTTGATGCG GCGGGGCGCG GGAAAATCGG GGAAGATGTT 601 TATGAGCATT GCCTCGGGTG TTATCAGATG GCCCAGGTAT ATTTGGCGAA 651 ATACCGGGAT GTCGCGAATG ACGAGCAGAA GGTTTGGGAC TTCCGCGAAG 701 AGAGCAACCG GATTGCATCG GACTCGCGCG ATTATGTGTT TTATCAGAAT 751 ATGCGGGAAT TGATGCCCCG GGGGatgaaG gcgaacagtc ttgtggtcgg 801 ctatgatgcg gacggtCtgc CgcaAAAagt ctattggagt gtcgacaatg 851 gaaaaaaacc ccaaagtgtc gaatattatt tgaaaaacgg aaatcttttt 901 attgcccaat cttcgacggt aaccttgaaa acggatggcg taacggcgga 951 tatgcaaacc tatcatgcgc aacaaacgtt gtatttggat ggg... This corresponds to the amino acid sequence <SEQ ID 2924; ORF 957. ng>: g957.pep (partial) 1 MFKKFKPVLL SFFALVFAFW LGTGIAYEIΝ PRWFLSDTAT EVPEΝPΝAFV 51 AKLARLFRΝA DRAVVIVKES MRTEESLAGA VDDGPLQSEK DYLALAIRLS 101 RLKEKAKWFH VTEQEHGEEV WLDYYIGEGG LVAVSLSQRS PEAFVΝAEYL 151 YRΝDRPFSVΝ VYGGTAHGEΝ YETTGEYRW WQPDGSVFDA AGRGKIGEDV 201 YEHCLGCYQM AQVYLAKYRD VAΝDEQKVWD FREESΝRIAS DSRDYVFYQΝ 251 MRELMPRGMK AΝSLVVGYDA DGLPQKVYWS VDΝGKKPQSV EYYLKΝGΝLF 301 IAQSSTVTLK TDGVTADMQT YHAQQTLYLD G... The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2925>: m957.seq 1 ATGTTTAAAA AATTCAAACC GGTACTGTTG TCATTTTTTG CACTTGTATT 51 TGCCTTTTGG CTGGGAACGG GTATTGCCTA TGAGATTAAT CCGCGTTGGT 101 TTTTGAGCGA TACGGCAACT GAAGTACCTA AAAATCCGAA TGCTTTTGTG 151 GCGAAACTTG CCCGCCTGTT CCGAAATGCC GACAGGGCGG TTGTCATCGT 201 GAAGGAATCG ATAAGGACGG AGGAAAATCT TGCCGGAACT GTGGATGACG 251 GTCCGTTGCA GTCGGAGAAG GATTATCTCG CGCTCGCTAT CCGGCTCAGT 301 CGTTTGAAAG AAAAGGCGAA ATGGTTTCAC GTAACGGAGC AGGAACATGG 351 GAAAGAGGTT TGGCTGGATT ACCATATCGG CGAGGGCGGT TTGGTTGCGG 401 TTTCGCTTTC GCAACGCTCG CCGGAAGCAT TTGTTAATGC CGAATATCTG 451 TATCGGAACG ATCGTCCGTT TTCTGTAAAT GTGTACGGCG GAACGGTTCA 501 CGGGGAAAAT TATGAAACGA CAGGAGAATA TCGGGTTGTT TGGCAACCAG 551 ACGGTTCGGT ATTTGATGCG GCGGGGCGCG GGAAAATCGG GGAAGATGTT 601 TATGAGCATT GCCTCGGGTG TTATCAGATG GCCCAGGTAT ATTTGGCGAA 651 ATACCGGGAT GTCGCGAATG ACGAGCAGAA GGTTTGGGAC TTCCGCAAAG 701 AGAGCAACCG AATTGCGTCG GACTCGCGCA ATTCTGTGTT TTATCAGAAT 751 ATGCGGGAAT TGATGCCCCG AGGGATGAAG GCGAACAGTC TTGTGGTCGG 801 CTATGATGCG GACGGTCTGC CGCAAAAAGT CTATTGGAGT TTCGACAATG 851 GAAAAAAACG CCAGAGTTTC GAATATTATT TGAAAAACGG AAATCTTTTT 901 ATTGCACAAT CTTCGACGGT AGCATTGAAA GCGGATGGCG TAACGGCGGA 951 TATGCAGACC TATCATGCGC AACAGACGTG GTATTTGGAT GGCGGGCGGA 1001 TTGTCCGCGA AGAGAAACAG GGAGACAGAC TGCCTGATTT TCCTTTGAAC 1051 TTGGAAAATT TGGAAAAAGA GGTGCGCCGT TATGCAGAGG CTGCGGCGAG 1101 ACGTTCGGGC GGCAGGCGCG ACCTTTCTCA CTGA This corresponds to the amino acid sequence <SEQ ID 2926; ORF 957>: m957.pep 1 MFKKFKPVLL SFFALVFAFW LGTGIAYEIΝ PRWFLSDTAT EVPKΝPΝAFV 51 AKLARLFRΝA DRAVVIVKES IRTEEΝLAGT VDDGPLQSEK DYLALAIRLS 101 RLKEKAKWFH VTEQEHGKEV WLDYHIGEGG LVAVSLSQRS PEAFVΝAEYL 151 YRΝDRPFSVΝ VYGGTVHGEΝ YETTGEYRW WQPDGSVFDA AGRGKIGEDV 201 YEHCLGCYQM AQVYLAKYRD VAΝDEQKVWD FRKEΞΝRIAS DSRΝSVFYQΝ 251 MRELMPRGMK ANSLWGYDA DGLPQKVYWS FDNGKKRQSF EYYLKNGNLF 301 IAQSSTVALK ADGVTADMQT YHAQQTWYLD GGRIVREEKQ GDRLPDFPLN 351 LENLEKEVRR YAEAAARRSG GRRDLSH* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 957 shows 95.2% identity over a 331 aa overlap with a predicted ORF (ORF 957) from N gonorrhoeae g957/m957 95.2% identity in 331 aa overlap 10 20 30 40 50 60 g957.pep MFKKFKPVLLSFFALVFAFWLGTGIAYEIΝPRWFLSDTATEVPEΝPΝAFVAKLARLFRΝA I II I I I I I I I I I I I I I I I I M I I I I I I I I I I I I I I I I I I I I I I : II I I I I I I I I I I I I I I m957 MFKKFKPVLLSFFALVFAFWLGTGIAYEIΝPRWFLSDTATEVPKΝPΝAFVAKLARLFRΝA 10 20 30 40 50 60 70 80 90 100 110 120 g957.pep DRAVVIVKESMRTEESLAGAVDDGPLQSEKDYLALAIRLSRLKEKAKWFHVTEQEHGEEV I I I I I I I I I M I I I M II M I I I II I II I II I I I I II I I I I I I I I I I I I II I I I I I I M I m957 DRAWIVKESIRTEEΝLAGTVDDGPLQSEKDYLALAIRLSRLKEKAKWFHVTEQEHGKEV 70 80 90 100 110 120 130 140 150 160 170 180 g957 . pep WLDYYIGEGGLVAVSLSQRSPEAFVΝAEYLYRΝDRPFSVΝVYGGTAHGEΝYETTGEYRW I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M I I I I I I I I I I I I I I m957 WLDYHIGEGGLVAVSLSQRSPEAFVΝAEYLYRΝDRPFSVΝVYGGTVHGEΝYETTGEYRW 130 140 150 160 170 180 190 200 210 220 230 240 g957.pep WQPDGSVFDAAGRGKIGEDVYEHCLGCYQMAQVYLAKYRDVAΝDEQKVWDFREESΝRIAS I I I I I I I I I I I II I I I I II I I I I I I I II I I I I I I I I I I II I II I I I I I I II I : I I I I I I I m957 WQPDGSVFDAAGRGKIGEDVYEHCLGCYQMAQVYLAKYRDVAΝDEQKVWDFRKESΝRIAS 190 200 210 220 230 240 250 260 270 280 290 300 g957.pep DSRDYVFYQΝMRELMPRGMKAΝSLVVGYDADGLPQKVYWSVDΝGKKPQSVEYYLKΝGΝLF III: I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I II llllllllll m957 DSRΝSVFYQΝMRELMPRGMKAΝSLVVGYDADGLPQKVYWSFDΝGKKRQSFEYYLKΝGΝLF 250 260 270 280 290 300 310 320 330 g957.pep IAQSSTVTLKTDGVTADMQTYHAQQTLYLDG II I I I I I : I M I I I I I I I I I I I I I M llll m957 IAQSSTVALKADGVTADMQTYHAQQTWYLDGGRIVREEKQGDRLPDFPLΝLEΝLEKEVRR 310 320 330 340 350 360 m957 YAEAAARRSGGRRDLSHX 370 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2927>: a957. seq 1 ATGTTTAAAA AATTCAAACC GGTACTGTTG TCATTTTTTG CACTTGTATT 51 TGCCTTTTGG CTGGGAACGG GTATTGCCTA TGAGATTAAT CCGCGTTGGT 101 TTTTGAGCGA TACGGCAACT GAAAATCCGA ATGCTTTTGT GGCGAAACTT 151 GCCCGCCTGT TCCGAAATGC CGACAGGGCG GTTGTCATCG TGAAGGAATC 201 GATGAGGACG GAGGAAAGTC TTGCCGGAGC TGTGGATGAC GGTCCGTTGC 251 AGTCGGAGAA GGATTATCTT GCACTCGCTG TCCGGCTCAG TCGTTTGAAA 301 GAAAAGGCGA AATGGTTTCA CGTAACGGAG CAGGAACATG GGGAAGAGGT 351 TTGGCTGGAT TACTATATCG GCGAGGGCGG TTTGGTTGCG GTTTCGCTTT 401 CGCAACGCTC GCCGGAAGCG TTTGTTAATG CCGAATATCT GTATCGGAAC 451 GATCGTCCGT TTTCTGTAAA TGTGTACGGC GGAACGGTTC ACGGGGAAAA 501 TTATGAAACG ACAGGAGAAT ATCGGGTTGT TTGGCAACCG GACGGTTCGG 551 TATTTGATGC GTCGGGGCGC GGGAAAATCG GGGAAGATGT TTATGAGCAT 601 TGCCTCGGGT GTTATCAGAT GGCCCAGGTA TATTTGGCGA AATATCGGGA 651 TGTCGCGAAT GATGAGCAGA AGGTTTGGGA CTTCCGCGAA GAGAGTAACC 701 GGATTGCGTC GGACTCGCGC GATTCTGTGT TTTATCAGAA TATGCGGGAA 751 TTGATGCCCC GAGGGATGAA GGCAAACAGT CTTGTGGTCG GCTATGATGC 801 GGACGGTCTG CCGCAGAAAG TCTATTGGAG TTTCGACAAT GGGAAAAAAC 851 GCCAGAGTTT CGAATATTAT TTGAAAAACG GAAATCTTTT TATTGCACAA 901 TCTTCGACGG TAGCATTGAA AGCGGATGGC GTAACGGCGG ATATGCAGAC 951 CTATCATGCG CAACAGACGT GGTATTTAGA TGGCGGGCGG ATTGTCCGCG 1001 AAGAGAAACA GGGGGACAGA CTGCCTGATT TTCCTTTGAA CTTGGAAGAT 1051 TTGGAAAAAG AGGTGAGCCG TTATGCAGAG GCTGCGGCGA GACGTTCGGG 1101 CGGCAGGCGC GACCTTTCTC ACTGA This corresponds to the amino acid sequence <SEQ ID 2928; ORF 957. a>: a957.pep 1 MFKKFKPVLL SFFALVFAFW LGTGIAYEIN PRWFLSDTAT ENPNAFVAKL 51 ARLFRNADRA VVIVKESMRT EESLAGAVDD GPLQSEKDYL ALAVRLSRLK 101 EKAKWFHVTE QEHGEEVWLD YYIGEGGLVA VSLSQRSPEA FVNAEYLYRN 151 DRPFSVNVYG GTVHGENYET TGEYRVVWQP DGSVFDASGR GKIGEDVYEH 201 CLGCYQMAQV YLAKYRDVAN DEQKVWDFRE ESNRIASDSR DSVFYQNMRE 251 LMPRGMKANS LWGYDADGL PQKVYWSFDN GKKRQSFEYY LKNGNLFIAQ 301 SSTVALKADG VTADMQTYHA QQTWYLDGGR IVREEKQGDR LPDFPLNLED 351 LEKEVSRYAE AAARRSGGRR DLSH* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 957 shows 96.3% identity over a 377 aa overlap with a predicted ORF (ORF 957) from N meningitidis a957/ 957 96.3% identity in 377 aa overlap 10 20 30 40 50 a957 . pep MFKKFKPVLLSFFALVFAFWLGTGIAYEIΝPRWFLSDTATE ΝPΝAFVAKLARLFRΝA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m957 MFKKFKPVLLSFFALVFAFWLGTGIAYEIΝPRWFLSDTATEVPKΝPΝAFVAKLARLFRΝA 10 20 30 40 50 60 60 70 80 90 100 110 a957 . pep DRAWIVKESMRTEESLAGAVDDGPLQSEKDYLALAVRLSRLKEKAKWFHVTEQEHGEEV I I I I I I I I I M I I I M I I I M I I I I I I I I I I I I I I M I I I I I I I I I I I I I I I I I I I M I I m957 DRAWIVKESIRTEEΝLAGTVDDGPLQSEKDYLALAIRLSRLKEKAKWFHVTEQEHGKEV 70 80 90 100 110 120 120 130 140 150 160 170 a957 . pep WLDYYIGEGGLVAVSLSQRSPEAFVΝAEYLYRΝDRPFSVΝVYGGTVHGEΝYETTGEYRW I I I I M I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m957 WLDYHIGEGGLVAVSLSQRSPEAFVΝAEYLYRΝDRPFSVΝVYGGTVHGEΝYETTGEYRW 130 140 150 160 170 180 180 190 200 210 220 230 a957. pep WQPDGSVFDASGRGKIGEDVYEHCLGCYQMAQVYLAKYRDVAΝDEQKVWDFREESΝRIAS I I I I I I II I Ml II I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I M I I I I I II m957 WQPDGSVFDAAGRGKIGEDVYEHCLGCYQMAQVYLAKYRDVAΝDEQKVWDFRKESΝRIAS 190 200 210 220 230 240 240 250 260 270 280 290 a957. pep DSRDSVFYQΝMRELMPRGMKAΝSLVVGYDADGLPQKVYWSFDΝGKKRQSFEYYLKΝGΝLF I I M I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m957 DΞRΝSVFYQΝMRELMPRGMKAΝSLWGYDADGLPQKVYWSFDΝGKKRQSFEYYLKΝGΝLF 250 260 270 280 290 300 300 310 320 330 340 350 a957 . pep IAQSSTVALKADGVTADMQTYHAQQTWYLDGGRIVREEKQGDRLPDFPLΝLEDLEKEVSR I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I m957 IAQΞSTVALKADGVTADMQTYHAQQTWYLDGGRIVREEKQGDRLPDFPLΝLEΝLEKEVRR 310 320 330 340 350 360 360 370 a957. pep YAEAAARRSGGRRDLSHX I I I I I I I I I II I I I I I I I m957 YAEAAARRSGGRRDLSHX 370 The following partial DΝA sequence was identified in N gonorrhoeae <SEQ ID 2929>: g958.seq 1 TTGGCTCGTT TATTTTCACT CAAACCACTG GTGCTGGCAT TGGGCTTCTG 51 TTTCGGCACG CATTGCGCCG CCGATACCGT TGCGGCGGAA GAGGCGGACG 101 GGCGTGTCGC AGAAGGCGGT GCGCAGGGCG CGTCCGAATC CGCACAAGCT 151 TCCGATTTGA CCCTCGGTTC GACCTGCCTG TTTTGCAGTA ACGAAAGCGG 201 CAGCCCCGAG AGAACCGAAG CCGCCGTCCA AGGCAGCGGC GAAGCATCCG 251 TCCCCGAAGA CTATACGCGC ATTGTTGCCG ACAGGATGGA AGGACAGTCG 301 AAGGTTAAGG TGCGCGCGGA AGGAAGCGTT ATCATCGAAC GGGACGGCGC 351 AGTCCTCAAT ACCGATTGGG CGGATTACGA CCAGTCGGGC GACACCGTTA 401 CCGTAGGCGA CCGGTTCGCC CTCCAACAGG ACGGTACGCT GATTCGGGGC 451 GAAACCCTGA CCTACAATCT CGATCAGCAG ACCGGCGAAG CGCACAACGT 501 CCGTATGGAA ACCGAACAAG GCGGACGGCG GCTGCAAAGC GTCAGCCGCA 551 CCGCCGAAAT GTTGGGCGAA GGGCGTTACA AACTGACGGA AACCCAATTC 601 AACACCTGTT CCGCCGGAGA TGCCGGCTGG TATGTCAAGG CCGCCTCTGT 651 CGAAGCCGAT CGGGGAAAAG GCATAGGCGT TGCCAAACAC GCCGCCTTCG 701 TGTTCGGCGG CGTTCCCCTT TTCTATACGC CTTGGGCGGA CTTCCCGCTT 751 GACGGCAACC GCAAAAGCGG ACTGCTCGTC CCGTCCGTAT CTGCCGGTTC 801 GGACGGCGTT TCCCTTTCCG TCCCCTATTA TTTCAACCTT GCCCCCAACT 851 TCGATGCCAC TTTCGCCCCC GGCATTATCG GCGAACGCGG CGCGACGTTT 901 GACGGACAAA TCCGTTACCT GCGTCCCGAT TACAGCGGAC AGACCGACCT 951 GACCTGGTTG CCGCACGATA AGAAAAGCGG CAGGAACAAC CGCTATCAGG 1001 CAAAATGGCA GCACCGGCAC GACATTTCCG ACACGCTTCA GGCGGGTGTC 1051 GATTTCAACC AAGTCTCCGA CAGCGGCTAC TACCGCGACT TTTACGGCGG 1101 CGAAGAAATC GCCGGCAACG TCAACCTCAA CCGCCGCGTA TGGCTGGATT 1151 ATGGCGGCAG GGCGGCGGGA GGCAGCCTGA ATGCCGGCCT TTCGGTTCAG 1201 AAATACCAGA CGCTGGCAAA CCAAAGCGGC TACAAAGACG AACCTTACGC 1251 CATCATGCCC CGCCTTTCTG CCGATTGGCA TAAAAACGCA GGCAGGGCGC 1301 AAATCGGCGT GTCCGCACAA TTTACCCGCT TCAGCCACGA CGGCCGCCAA 1351 GACGGCAGCC GACTGGTCGT GTATCCCGGT ATCAAATGGG ATTTCAGCAA 1401 CAGCTGGGGC TACGTCCGCC CCAAACTCGG GCTGCACGCC ACTTATTACA 1451 GCCTCGACAG TTTCGGCGGC AAAGCATCCC GCAGCGTCGG GCGCGTTTTG 1501 CCCGTTGTCA ATATCGACGG CGGCACAACC TTCGAACGCA ATACGCGCCT 1551 GTTCGGCGGC GGAGTCGTGC AAACCATCGA GCCGCGCCTG TTCTACAACT 1601 ATATTCCTGC CAAATCTCAA AACGACCTGC CCAATTTCGA TTCGTCGGAA 1651 AGCAGCTTCG GCTACGGGCA GCTTTTCCGC GAAAACCTCT ATTACGGCAA 1701 CGACCGCATC AACGCCGCCA ACAGCCTTTC CACCGCCGTG CAGAGCCGTA 1751 TTTTGGACGG CGCGACGGGG GAGGAGCGTT TCCGCGCCGG TATCGGTCAG 1801 AAATTCTATT TCAAGGATGA TGCGGTGATG CTTGACGGCA GCGTCGGCAA 1851 AAATCCGCGC AGCCGTTCCG ACTGGGTGGC ATTCGCCTCC GGCGGCATAG 1901 GCGGGCGTTT CACCCTCGAC AGCAGCATCC ACTACAACCA AAACGACAAA 1951 CGCGCCGAAC ATTACGCCGT CGGCGCAGGC TACCGCCCCG CCCCCGGAAA 2001 AGTGTTGAAC GCCCGCTACA AATACGGGCG CAACGAAAAA ATCTACCTGC 2051 AGGCGGACGG TTCCTATTTT TACGACAAAC TCAGCCAGCT CGACCTGTCC 2101 GCACAATGGC CGCTGACGCG CAACCTGTCT GCCGTCGTCC GCTACAACTA 2151 CGGTTTTGAA GCCAAAAAAC CGATAGAAAT GCTTGCCGGT GCAGAATACA 2201 AAAGCAGTTG CGGCTGCTGG GGCGCGGGCG TGTACGCCCA ACGCTACGTT 2251 ACCGGCGAAA ACACCTACAA AAACGCCGTC TTTTTTTCAC TTCAGTTGAA 2301 AGACCTCAGC AGCGTCGGCA GAAACCCCGC AGGCAGGATG GATGTCGCCG 2351 TTCCCGGCTA CATCCCCGCC CACTCTCTTT CCGCCGGACG CAACAAACGG 2401 CCCTGA This corresponds to the amino acid sequence <SEQ ID 2930; ORF 958.ng>: g958.pep 1 LARLFSLKPL VLALGFCFGT HCAADTVAAE EADGRVAEGG AQGASESAQA 51 SDLTLGΞTCL FCSNESGSPE RTEAAVQGSG EASVPEDYTR IVADRMEGQS 101 KVKVRAEGSV IIERDGAVLN TDWADYDQSG DTVTVGDRFA LQQDGTLIRG 151 ETLTYNLDQQ TGEAHNVRME TEQGGRRLQS VSRTAEMLGE GRYKLTETQF 201 NTCSAGDAGW YVKAASVEAD RGKGIGVAKH AAFVFGGVPL FYTPWADFPL 251 DGNRKSGLLV PSVSAGSDGV SLSVPYYFNL APNFDATFAP GIIGERGATF 301 DGQIRYLRPD YSGQTDLTWL PHDKKSGRNN RYQAKWQHRH DISDTLQAGV 351 DFNQVSDSGY YRDFYGGEEI AGNVNLNRRV WLDYGGRAAG GSLNAGLSVQ 401 KYQTLANQSG YKDEPYAIMP RLSADWHKNA GRAQIGVSAQ FTRFSHDGRQ 451 DGSRLVVYPG IKWDFSNSWG YVRPKLGLHA TYYSLDSFGG KASRSVGRVL 501 P NIDGGTT FERNTRLFGG GVVQTIEPRL FYNYIPAKSQ NDLPNFDSSE 551 SSFGYGQLFR ENLYYGNDRI NAANSLSTAV QSRILDGATG EERFRAGIGQ 601 KFYFKDDAVM LDGSVGKNPR SRSDWVAFAS GGIGGRFTLD SSIHYNQNDK 651 RAEHYAVGAG YRPAPGKVLN ARYKYGRNEK IYLQADGSYF YDKLSQLDLS 701 AQWPLTRNLS AWRYNYGFE AKKPIEMLAG AEYKSSCGCW GAGVYAQRYV 751 TGENTYKNAV FFSLQLKDLS SVGRNPAGRM DVAVPGYIPA HSLSAGRNKR 801 P* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2931>: m958.seq 1 TTGGCTCGTT TATTTTCACT CAAACCACTG GTGCTGGCAT TGGGCCTCTG 51 CTTCGGCACG CATTGCGCCG CCGCCGATGC CGTTGCGGCG GAGGAAACGG 101 ACAATCCGAC CGCCGGAGAA AGCGTTCGGA GCGTGTCCGA ACCCATACAG 151 CCTACCAGCC TGAGCCTCGG TTCGACCTGC CTGTTTTGCA GTAACGAAAG 201 CGGCAGCCCC GAGAGAACCG AAGCCGCCGT CCAAGGCAGC GGCGAAGCAT 251 CCATCCCCGA AGACTATACG CGCATTGTTG CCGACAGGAT GGAAGGACAG 301 TCGCAGGTGC AGGTGCGTGC CGAAGGCAAC GTCGTCGTCG AACGCAACCG 351 GACGACCCTC AATACCGATT GGGCGGATTA CGACCAGTCG GGCGACACCG 401 TTACCGCAGG CGACCGGTTC GCCCTCCAAC AGGACGGTAC GCTGATTCGG 451 GGCGAAACCC TGACCTACAA TCTCGAGCAG CAGACCGGGG AAGCGCACAA 501 CGTCCGCATG GAAATCGAAC AAGGCGGACG GCGGCTGCAA AGCGTCAGCC 551 GCACCGCCGA AATGTTGGGC GAAGGGCATT ACAAACTGAC GGAAACCCAA 601 TTCAACACCT GTTCCGCCGG CGATGCCGGC TGGTATGTCA AGGCAGCCTC 651 TGTCGAAGCC GATCGGGAAA AAGGCATAGG CGTTGCCAAA CACGCCGCCT 701 TCGTGTTCGG CGGCGTTCCC ATTTTCTACA CCCCTTGGGC GGACTTCCCG 751 CTTGACGGCA ACCGCAAAAG CGGCCTGCTT GTTCCCTCAC TGTCCGCCGG 801 TTCGGACGGC GTTTCCCTTT CCGTTCCCTA TTATTTCAAC CTTGCCCCCA 851 ATCTCGATGC CACGTTCGCG CCCAGCGTGA TCGGCGAACG CGGCGCGGTC 901 TTTGACGGGC AGGTACGCTA CCTGCGGCCG GATTATGCCG GCCAGTCCGA 951 CCTGACCTGG CTGCCGCACG ACAAGAAAAG CGGCAGGAAT AACCGCTATC 1001 AGGCGAAATG GCAGCATCGG CACGACATTT CCGACACGCT TCAGGCGGGT 1051 GTCGATTTCA ACCAAGTCTC CGACAGCGGC TACTACCGCG ACTTTTACGG 1101 CAACAAAGAA ATCGCCGGCA ACGTCAACCT CAACCGCCGT GTATGGCTGG 1151 ATTATGGCGG CAGGGCGGCG GGCGGCAGCC TGAATGCCGG CCTTTCGGTT 1201 CTGAAATACC AGACGCTGGC AAACCAAAGC GGCTACAAAG ACAAACCGTA 1251 TGCCCTCATG CCGCGCCTTT CGGTCGAGTG GCGTAAAAAC ACCGGCAGGG 1301 CGCAAATCGG CGTGTCCGCA CAATTTACCC GATTCAGCCA CGACAGCCGC 1351 CAAGACGGCA GCCGCCTGGT CGTCTATCCC GACATCAAAT GGGATTTCAG 1401 CAACAGCTGG GGCTATGTCC GTCCCAAACT CGGACTGCAC GCCACCTATT 1451 ACAGCCTCAA CCGCTTCGGC AGCCAAGAAG CCCGACGCGT CAGCCGCACT 1501 CTGCCCATTG TCAACATCGA CAGCGGCGCA ACTTTTGAGC GGAATACGCG 1551 GATGTTCGGC GGAGAAGTCC TGCAAACCCT CGAGCCGCGC CTGTTCTACA 1601 ACTATATTCC TGCCAAATCC CAAAACGACC TGCCCAATTT CGATTCGTCG 1651 GAAAGCAGCT TCGGCTACGG GCAGCTCTTT CGCGAAAACC TCTATTACGG 1701 CAACGACAGG ATTAACACCG CAAACAGCCT TTCCGCCGCC GTGCAAAGCC 1751 GTATTTTGGA CGGCGCGACG GGGGAAGAGC GTTTCCGCGC CGGCATCGGT 1801 CAGAAATTCT ATTTCAAGGA TGATGCGGTG ATGCTTGACG GCAGCGTCGG 1851 CAAAAAACCG CGCAACCGTT CCGACTGGGT GGCATTTGCC TCCGGCAGCA 1901 TCGGCAGCCG CTTCATCCTC GACAGCAGCA TCCACTACAA CCAAAACGAC 1951 AAACGCGCCG AGAACTACGC CGTCGGTGCA AGCTACCGTC CCGCACAGGG 2001 CAAAGTGCTG AACGCCCGCT ACAAATACGG GCGCAACGAA AAAATCTACC 2051 TGAAGTCCGA CGGTTCCTAT TTTTACGACA AACTCAGCCA GCTCGACCTG 2101 TCCGCACAAT GGCCGCTGAC GCGCAACCTG TCGGCCGTCG TCCGTTACAA 2151 CTACGGTTTT GAAGCCAAAA AACCGATAGA GGTGCTGGCG GGTGCGGAAT 2201 ACAAAAGCAG TTGCGGCTGC TGGGGCGCGG GCGTGTACGC CCAACGCTAC 2251 GTTACCGGCG AAAACACCTA CAAAAACGCT GTCTTTTTCT CACTTCAGTT 2301 GAAAGACCTC AGCAGTGTCG GCAGAAACCC CGCAGACAGG ATGGATGTCG 2351 CCGTTCCCGG CTATATCACC GCCCACTCTC TTTCCGCCGG ACGCAACAAA 2401 CGACCCTGA This corresponds to the amino acid sequence <SEQ ID 2932; ORF 958>: m958.pep 1 LARLFSLKPL VLALGLCFGT HCAAADAVAA EETDNPTAGE SVRSVSEPIQ 51 PTSLSLGSTC LFCSNESGSP ERTEAAVQGS GEASIPEDYT RIVADRMEGQ 101 SQVQVRAEGN VWERNRTTL NTDWADYDQS GDTVTAGDRF ALQQDGTLIR 151 GETLTYNLEQ QTGEAHNVRM EIEQGGRRLQ SVΞRTAEMLG EGHYKLTETQ 201 FNTCSAGDAG WYVKAASVEA DREKGIGVAK HAAFVFGGVP IFYTPWADFP 251 LDGNRKSGLL VPSLSAGSDG VSLΞVPYYFN LAPNLDATFA PSVIGERGAV 301 FDGQVRYLRP DYAGQΞDLTW LPHDKKSGRN NRYQAKWQHR HDISDTLQAG 351 VDFNQVSDSG YYRDFYGNKE IAGNVNLNRR VWLDYGGRAA GGSLNAGLSV 401 LKYQTLANQS GYKDKPYALM PRLSVEWRKN TGRAQIGVΞA QFTRFSHDSR 451 QDGSRLWYP DIKWDFSNSW GYVRPKLGLH ATYYΞLNRFG SQEARRVSRT 501 LPIVNIDSGA TFERNTRMFG GEVLQTLEPR LFYNYIPAKS QNDLPNFDSS 551 ESSFGYGQLF RENLYYGNDR INTANSLSAA VQSRILDGAT GEERFRAGIG 601 QKFYFKDDAV MLDGSVGKKP RNRSDWVAFA SGSIGSRFIL DSSIHYNQND 651 KRAENYAVGA SYRPAQGKVL NARYKYGRNE KIYLKSDGSY FYDKLSQLDL 701 SAQWPLTRNL SAVVRYNYGF EAKKPIEVLA GAEYKSSCGC WGAGVYAQRY 751 VTGENTYKNA VFFSLQLKDL SSVGRNPADR MDVAVPGYIT AHSLSAGRNK 801 RP* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 958 shows 89.3% identity over a 802 aa overlap with a predicted ORF (ORF 958) from N gonorrhoeae m958/g958 89.3% identity in 802 aa overlap 10 20 30 40 50 60 m958.pep LARLFSLKPLVLALGLCFGTHCAAADAVAAEETDNPTAGESVRSVSEPIQPTSLSLGSTC I I I I I I I I I I I I I I I : I I I II I I I I : I I I I M I : : I ::::: I I I :: I : I I II I g958 LARLFSLKPLVLALGFCFGTHCAA-DTVAAEEADGRVAEGGAQGASEΞAQASDLTLGSTC 10 20 30 40 50 70 80 90 100 110 120 m958.pep LFCSNESGSPERTEAAVQGSGEASIPEDYTRIVADRMEGQSQVQVRAEGNVWERNRTTL I I I I I I I I I I I I I I II I I I I I I I Ml I I I I I I I I I I I I I I M I M I I I M MM I : :: I g 958 FCSNESGSPERTEAAVQGSGEASVPEDYTRIVADRMEGQSKVKVRAEGSVIIERDGAVL 60 70 80 90 100 110 130 140 150 160 170 180 m958.pep NTDWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQQTGEAHNVRMEIEQGGRRLQ I I I I I I I I I I I I I I M I I I I I I I 1 I I I I I II I I I I I I I : I I I I I I I I I I II llllllll g958 NTDWADYDQSGDTVTVGDRFALQQDGTLIRGETLTYNLDQQTGEAHNVRMETEQGGRRLQ 120 130 140 150 160 170 190 200 210 220 230 240 m958.pep SVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASVEADREKGIGVAKHAAFVFGGVP I I I I I I I I I I I I : I II I I I I I I I I I I I I I I I I I I I I I I I I I I I II II I I I I I I I I I I I I g958 SVSRTAEMLGEGRYKLTETQFNTCSAGDAGWYVKAASVEADRGKGIGVAKHAAFVFGGVP 180 190 200 210 220 230 250 260 270 280 290 300 m958.pep IFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVPYYFNLAPNLDATFAPSVIGERGAV : I I I I I I II I I I I I II I I II I I I : I I I I I I I I I I I I I I I I I I I I M I I I I \ : : \ I II I I : g958 FYTPWADFPLDGNRKSGLLVPSVSAGSDGVSLSVPYYFNLAPNFDATFAPGIIGERGAT 240 250 260 270 280 290 310 320 330 340 350 360 m958.pep FDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQAKWQHRHDIΞDTLQAGVDFNQVSDSG I I I I : I I I I I I I : I Ml I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g958 FDGQIRYLRPDYSGQTDLTWLPHDKKSGRNNRYQAKWQHRHDISDTLQAGVDFNQVSDSG 300 310 320 330 340 350 370 380 390 400 410 420 m958. pep YYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGGSLNAGLSVLKYQTLANQSGYKDKPYALM I I I I I I MM I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I : I II M g958 YYRDFYGGEEIAGNVNLNRRVWLDYGGRAAGGΞLNAGLSVQKYQTLANQSGYKDEPYAIM 360 370 380 390 400 410 430 440 450 460 470 480 m958.pep PRLSVEWRKNTGRAQIGVSAQFTRFSHDSRQDGSRLWYPDIKWDFSNSWGYVRPKLGLH I I I MM : I Ml I I II I I I I I I I I I I I M I I I I I I I I I II I I I I I I I I I I I I I I I I I I I g958 PRLSADWHKNAGRAQIGVSAQFTRFSHDGRQDGSRLWYPGIKWDFSNSWGYVRPKLGLH 420 430 440 450 460 470 490 500 510 520 530 540 m958.pep ATYYSLNRFGSQEARRVSRTLPIVNIDSGATFERNTRMFGGEVLQTLEPRLFYNYIPAKS MIIIM I I :: M M M I M I I I I : M II I II I M I I I I : I I : I I I I I I I I I I I I I g958 ATYYSLDSFGGKASRSVGRVLPVVNIDGGTTFERNTRLFGGGVVQTIEPRLFYNYIPAKS 480 490 500 510 520 530 550 560 570 580 590 600 m958.pep QNDLPNFDSSESSFGYGQLFRENLYYGNDRINTANSLSAAVQSRILDGATGEERFRAGIG I I II I I I I I I I I I I II I II I I I I I I I I II II I: I I I I I : I I I I I II I I I I I I I I I I I I I I g958 QNDLPNFDSSESSFGYGQLFRENLYYGNDRINAANSLSTAVQSRILDGATGEERFRAGIG 540 550 560 570 580 590 610 620 630 640 650 660 m958.pep QKFYFKDDAVMLDGSVGKKPRNRSDWVAFASGΞIGSRFILDSSIHYNQNDKRAENYAVGA I I I I I I I I I II I II I I I M I I : I I I I I I I I I I M I : I I I I I I I I I I I I I I I I I : II II I g958 QKFYFKDDAVMLDGSVGKNPRSRSDWVAFASGGIGGRFTLDSSIHYNQNDKRAEHYAVGA 600 610 620 630 640 650 670 680 690 700 710 720 m958.pep SYRPAQGKVLNARYKYGRNEKIYLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGF ill II I I I I I I I I I I I I I I I I :: I II I I II I I I I I I I I II I II I I I I I I I I I I I I | | g958 GYRPAPGKVLNARYKYGRNEKIYL Q ADGSYFYDKLSQLDLSAQWPLTRNLSAWRYNYGF 660 670 680 690 700 710 730 740 750 760 770 780 m958.pep EAKKPIEVLAGAEYKSSCGCWGAGVYAQRYVTGENTYKNAVFFSLQLKDLΞSVGRNPADR I I II I I Ml I I I I I II I I II I I I II I I I I I II I I I I I I I I I I II I I I I I M I I I I I I I I g958 EAKKPIEMLAGAEYKSSCGCWGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPAGR 720 730 740 750 760 770 790 800 m958.pep MDVAVPGYITAHSLSAGRNKRP lllllllll I II I I I I I I I II g958 MDVAVPGYIPAHSLSAGRNKRPX 780 790 800 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2933>: a958.seq 1 TTGGCTCGTT TATTTTCACT CAAACCACTG GTGCTGGCAT TGGGCTTCTG 51 TTTCGGCACG CATTGCGCCG CCGCCGATGC CGTTGCGGCG GAGGAAACGG 101 ACAATCCGAC CGCCGGAGGA AGCGTTCGGA GCGTGTCCGA ACCCATACAG 151 CCTACCAGCC TGAGCCTCGG TTCGACCTGC CTGTTTTGCA GTAACGAAAG 201 CGGCAGCCCC GAGAGAACCG AAGCCGCCGT CCAAGGCAGC GGCGAAGCAT 251 CCATCCCCGA AGACTATACG CGCATTGTTG CCGACAGGAT GGAAGGACAG 301 TCGCAGGTGC AGGTGCGTGC CGAAGGCAAC GTCGTCGTCG AACGCAATCG 351 GACGACCCTC AATGCCGATT GGGCGGATTA CGACCAGTCG GGCGACACCG 401 TTACCGCAGG CGACCGGTTC GCCCTCCAAC AGGACGGTAC GCTGATTCGG 451 GGCGAAACCC TGACCTACAA TCTCGAGCAG CAGACCGGGG AAGCGCACAA 501 CGTCCGTATG GAAACCGAAC ACGGCGGACG GCGGCTGCAA AGCGTCAGCC 551 GCACCGCCGA AATGTTGGGC GAAGGGCATT ACAAACTGAC GGAAACCCAA 601 TTCAACACCT GTTCCGCCGG CGATGCCGGC TGGTATGTCA AGGCCGCTTC 651 CGTCGAAGCC GATCGGGAAA AAGGCATAGG CGTTGCCAAA CACGCCGCCT 701 TCGTGTTCGG CGGCGTTCCC ATTTTCTACA CCCCTTGGGC GGACTTCCCG 751 CTTGACGGCA ACCGCAAAAG CGGCCTGCTC GTTCCCTCAC TGTCCGCCGG 801 TTCGGACGGC GTTTCCCTTT CCGTTCCCTA TTATTTCAAC CTTGCCCCCA 851 ATCTCGATGC CACGTTCGCG CCCGGCGTGA TCGGCGAACG CGGCGCGGTC 901 TTTGACGGGC AGGTACGCTA CCTGCGGCCG GATTATGCCG GCCAGTCCGA 951 CCTGACCTGG CTGCCGCACG ACAAGAAAAG CGGCAGGAAT AACCGCTATC 1001 AGGCGAAATG GCAGCACCGG CACGACATTT CCGACACGCT TCAGGCGGGT 1051 GTCGATTTCA ACCAAGTCTC CGACAGCGGC TACTACCGCG ACTTTTACGG 1101 CAACAAAGAA ATCGCCGGCA ACGTCAACCT CAACCGCCGT GTATGGCTGG 1151 ATTATGGCGG CAGGGCGGCG GGCGGCAGCC TGAATGCCGG CCTTTCGGTT 1201 CTGAAATACC AGACGCTGGC AAACCAAAGC GGCTACAAAG ACAAACCGTA 1251 TGCCCTGATG CCGCGCCTTT CCGCCGATTG GCGCAAAAAC ACCGGCAGGG 1301 CGCAAATCGG CGTGTCCGCC CAATTTACCC GCTTCAGCCA CGACAGCCGC 1351 CAAGACGGCA GCCGCCTCGT CGTCTATCCC GACATCAAAT GGGATTTCAG 1401 CAACAGCTGG GGTTACGTCC GTCCCAAACT CGGACTGCAC GCCACCTATT 1451 ACAGCCTCAA CCGCTTCGGC AGCCAAGAAG CCCGACGCGT CAGCCGCACT 1501 CTGCCCATCG TCAACATCGA CAGCGGCATG ACCTTCGAAC GCAATACGCG 1551 GATGTTCGGC GGCGGAGTCC TGCAAACCCT CGAGCCGCGC CTGTTCTACA 1601 ACTATATTCC TGCCAAATCC CAAAACGACC TGCCCAATTT CGATTCGTCG 1651 GAAAGCAGCT TCGGCTACGG GCAGCTTTTT CGTGAAAACC TCTATTACGG 1701 CAACGACAGG ATTAACACCG CAAACAGCCT TTCCGCCGCC GTGCAAAGCC 1751 GTATTTTGGA CGGCGCGACG GGGGAAGAGC GTTTCCGCGC CGGCATCGGG 1801 CAGAAATTCT ACTTCAAAAA CGACGCAGTC ATGCTTGACG GCAGTGTCGG 1851 CAAAAAACCG CGCAGCCGTT CCGACTGGGT GGCATTCGCC TCCAGCGGCA 1901 TCGGCAGCCG CTTCATCCTC GACAGCAGCA TCCACTACAA CCAAAACGAC 1951 AAACGCGCCG AGAACTACGC CGTCGGTGCA AGCTACCGTC CCGCACAGGG 2001 CAAAGTGCTG AACGCCCGCT ACAAATACGG GCGCAACGAA AAAATCTACC 2051 TGAAGTCCGA CGGTTCCTAT TTTTACGACA AACTCAGCCA GCTCGACCTG 2101 TCCGCACAAT GGCCGCTGAC GCGCAACCTG TCGGCCGTCG TCCGTTACAA 2151 CTACGGTTTT GAAGCCAAAA AACCGATAGA GGTGCTGGCG GGTGCGGAAT 2201 ACAAAAGCAG TTGCGGCTGC TGGGGCGCGG GCGTGTACGC CCAACGCTAC 2251 GTTACCGGCG AAAACACCTA CAAAAACGCT GTCTTTTTCT CACTTCAGTT 2301 GAAAGACCTC AGCAGTGTCG GCAGAAACCC CGCAGACAGG ATGGATGTCG 2351 CCGTTCCCGG CTATATCCCC GCCCACTCTC TTTCCGCCGG ACGCAACAAA 2401 CGGCCCTGA This corresponds to the amino acid sequence <SEQ ID 2934; ORF 958. a>: a958.pep 1 LARLFΞLKPL VLALGFCFGT HCAAADAVAA EETDΝPTAGG SVRSVSEPIQ 51 PTSLSLGSTC LFCSΝESGSP ERTEAAVQGS GEASIPEDYT RIVADRMEGQ 101 SQVQVRAEGΝ VWERΝRTTL ΝADWADYDQS GDTVTAGDRF ALQQDGTLIR 151 GETLTYΝLEQ QTGEAHΝVRM ETEHGGRRLQ SVSRTAEMLG EGHYKLTETQ 201 FΝTCSAGDAG WYVKAASVEA DREKGIGVAK HAAFVFGGVP IFYTPWADFP 251 LDGNRKSGLL VPSLSAGSDG VSLSVPYYFN LAPNLDATFA PGVIGERGAV 301 FDGQVRYLRP DYAGQSDLTW LPHDKKSGRN NRYQAKWQHR HDISDTLQAG 351 VDFNQVSDSG YYRDFYGNKE IAGNVNLNRR VWLDYGGRAA GGSLNAGLSV 401 LKYQTLANQS GYKDKPYALM PRLSADWRKN TGRAQIGVSA QFTRFSHDSR 451 QDGSRLWYP DIKWDFSNSW GYVRPKLGLH ATYYSLNRFG SQEARRVSRT 501 LPIVNIDSGM TFERNTRMFG GGVLQTLEPR LFYNYIPAKS QNDLPNFDSS 551 ESΞFGYGQLF RENLYYGNDR INTANSLSAA VQSRILDGAT GEERFRAGIG 601 QKFYFKNDAV MLDGSVGKKP RSRSDWVAFA SSGIGSRFIL DSSIHYNQND 651 KRAENYA GA SYRPAQGKVL NARYKYGRNE KIYLKSDGSY FYDKLSQLDL 701 SAQWPLTRNL SAVVRYNYGF EAKKPIEVLA GAEYKSSCGC WGAGVYAQRY 751 VTGENTYKNA VFFSLQLKDL ΞSVGRNPADR MDVAVPGYIP AHSLSAGRNK 801 RP* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N. meningitidis ORF 957 shows 96.3% identity over a 377 aa overlap with a predicted ORF (ORF 957) from N meningitidis a958/m958 98.1% identity in 802 aa overlap 10 20 30 40 50 60 a958.pep ARLFSLKPLVLALGFCFGTHCAAADAVAAEETDΝPTAGGSVRSVSEPIQPTSLSLGSTC I I I I I I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I I II I I I I II I I II II I I I I I I m958 LARLFSLKPLVLALGLCFGTHCAAADAVAAEETDΝPTAGESVRSVSEPIQPTSLSLGSTC 10 20 30 40 50 60 70 80 90 100 110 120 a958.pep LFCSΝESGSPERTEAAVQGSGEASIPEDYTRIVADRMEGQSQVQVRAEGΝVWERΝRTTL I I I I I I I I I II II I II I II I I I I I I I I I I I I I I II I I I I I I I II I I I I I II I II I I I I I I m958 LFCSΝESGSPERTEAAVQGSGEASIPEDYTRIVADRMEGQSQVQVRAEGΝVWERΝRTTL 70 80 90 100 110 120 130 140 150 160 170 180 a958.pep ΝADWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYΝLEQQTGEAHΝVRMETEHGGRRLQ I : I I I I I I I I I II I I I I I I I I I I I I I I I I I I II I I I I I I II I II I I I I I II M I I I I I I m958 ΝTDWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYΝLEQQTGEAHΝVRMEIEQGGRRLQ 130 140 150 160 170 180 190 200 210 220 230 240 a958. pep SVSRTAEMLGEGHYKLTETQFΝTCSAGDAGWYVKAASVEADREKGIGVAKHAAFVFGGVP I I I I I II I I II I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I II I I I I Itι958 SVSRTAEMLGEGHYKLTETQFΝTCSAGDAGWYVKAASVEADREKGIGVAKHAAFVFGGVP 190 200 210 220 230 240 250 260 270 280 290 300 a958.pep IFYTPWADFPLDGΝRKSGLLVPSLSAGSDGVSLSVPYYFΝLAPΝLDATFAPGVIGERGAV I I I I I I I I I I I II I I I I I I I II I II I I I I I II I I I I I I I II I I I I II I I I I : II I II I I I m958 IFYTPWADFPLDGΝRKSGLLVPSLSAGSDGVSLSVPYYFΝLAPΝLDATFAPSVIGERGAV 250 260 270 280 290 300 310 320 330 340 350 360 a958.pep FDGQVRYLRPDYAGQSDLTWLPHDKKSGRΝΝRYQAKWQHRHDIΞDTLQAGVDFΝQVSDSG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I II I I I I I I I I I I m958 FDGQVRYLRPDYAGQSDLTWLPHDKKSGRΝΝRYQAKWQHRHDISDTLQAGVDFΝQVSDSG 310 320 330 340 350 360 370 380 390 400 410 420 a958.pep YYRDFYGΝKEIAGΝVΝLΝRRVWLDYGGRAAGGSLΝAGLSVLKYQTLAΝQSGYKDKPYALM I I I I I I I I I I I I I I I I I I I I I II II I I II I II I I I I I I I I I I I I I I I I I I I I I I I I I I 11 m958 YYRDFYGΝKEIAGΝVΝLΝRRVWLDYGGRAAGGSLΝAGLSVLKYQTLAΝQSGYKDKPYALM 370 380 390 400 410 420 430 440 450 460 470 480 a958.pep PRLSADWRKΝTGRAQIGVSAQFTRFSHDSRQDGSRLWYPDIKWDFSΝSWGYVRPKLGLH I I II : : I I I II I I I I I I II I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I II I II I II m958 PRLSVEWRKΝTGRAQIGVSAQFTRFSHDΞRQDGSRLWYPDIKWDFSΝSWGYVRPKLGLH 430 440 450 460 470 480 490 500 510 520 530 540 a958.pep ATYYSLΝRFGSQEARRVSRTLPIVΝIDSGMTFERΝTRMFGGGVLQTLEPRLFYΝYIPAKS I I I I I II I I I I I I I II I I I I I II II I II I I I I I I I I I I II I I I I II II I I I I I I I I ll m958 ATYYSLΝRFGSQEARRVSRTLPIVΝIDSGATFERΝTRMFGGEVLQTLEPRLFYΝYIPAKS 490 500 510 520 530 540 550 560 570 580 590 600 a958.pep QNDLPNFDSSESSFGYGQLFRENLYYGNDRINTANSLSAAVQSRILDGATGEERFRAGIG I I II I I I I I I I I I I II I I I I I I I II I I I I I I I II I II I I I I II I I I II I II I I I I II I I I m958 QNDLPNFDSSESSFGYGQLFRENLYYGNDRINTANSLSAAVQSRILDGATGEERFRAGIG 550 560 570 580 590 600 610 620 630 640 650 660 a958.pep QKFYFKNDAVMLDGSVGKKPRSRSDWVAFASSGIGSRFILDSΞIHYNQNDKRAENYAVGA I I II I M I I I I I I I I I I M I M I I I I II I I MM I II I I I I I I I I I I II M I I I I I I I I I m958 QKFYFKDDAVMLDGSVGKKPRNRSDWVAFASGSIGSRFILDSSIHYNQNDKRAENYAVGA 610 620 630 640 650 660 670 680 690 700 710 720 a958.pep SYRPAQGKVLNARYKYGRNEKIYLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGF I I I I I I I I I I I I I I I I I I I I I I I I I I I I I Ii I I I I II I II I II I I I I I I I I I I I I I I II I m958 SYRPAQGKVLNARYKYGRNEKIYLKΞDGΞYFYDKLSQLDLSAQWPLTRNLSAWRYNYGF 670 680 690 700 710 720 730 740 750 760 770 780 a958.pep EAKKPIEVLAGAEYKSSCGCWGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADR 111111111111111111111111111111111111111111111111111111111111 958 EAKKPIEVLAGAEYKSSCGCWGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADR 730 740 750 760 770 780 790 800 a958.pep MDVAVPGYIPAHSLSAGRNKRPX lllllllll I I I I I I I I I I I I m958 MDVAVPGYITAHSLSAGRNKRP 790 800 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2935>: g959.seq 1 ATGAACATCA AACACCTTCT CTTGACCGCC GCCGCAACCG CACTGTTGGG 51 CATTTCCGCC CCCGCACTCG CCCACCACGA CGGACACGGC GATGACGACC 101 ACGGACACGC CGCACACCAA CACGGCAAAC AAGACAAAAT CATCAGCCGC 151 GCCCAAGCCG AAAAAGCGGC TTGGGCGCGT GTCGGCGGCA AAATCACCGA 201 CATCGATCTC GAACACGACG ACGGCCGTCC GCACTATGAT GTCGAAATCG 251 TCAAAAACGG ACAGGAATAC AAAGTCGTTG TCGATGCCCG TACCGGCCGC 301 GTGATTTCCT CCCGCCGCGA CGACTGA This corresponds to the amino acid sequence <SEQ ID 2936; ORF 959.ng>: g959.pep 1 MΝIKHLLLTA AATALLGISA PALAHHDGHG DDDHGHAAHQ HGKQDKIISR 51 AQAEKAAWAR VGGKITDIDL EHDDGRPHYD VEIVKΝGQEY KVWDARTGR 101 VISSRRDD* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2937>: 959. seq 1 ATGAACATCA AACACCTTCT CTTGACCTCC GCCGCAACCG CACTGCTGAG 51 CATTTCCGCC CCCGCGCTCG CCCACCACGA CGGACACGGC GATGACGACC 101 ACGGACACGC CGCACACCAA CACAACAAAC AAGACAAAAT CATCAGCCGC 151 GCCCAAGCCG AAAAAGCAGC GTTGGCGCGT GTCGGCGGCA AAATCACCGA 201 CATCGATCTC GAACACGACA ACGGCCGTCC GCACTATGAT GTCGAAATCG 251 TCAAAAACGG ACAGGAATAC AAAGTCGTTG TCGATGCCCG TACCGGCCGC 301 GTGATTTCCT CCCGCCGCGA CGACTGA This corresponds to the amino acid sequence <SEQ ID 2938; ORF 959>: m959.peρ 1 MΝIKHLLLTS AATALLΞISA PALAHHDGHG DDDHGHAAHQ HΝKQDKIISR 51 AQAEKAALAR VGGKITDIDL EHDΝGRPHYD VEIVKΝGQEY KVWDARTGR 101 VISSRRDD* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 959 shows 95.4% identity over a 108 aa overlap with a predicted ORF (ORF 959) from N gonorrhoeae m959/g959 95.4% identity m 108 aa overlap 10 20 30 40 50 60 m959.pep MNIKHLLLTSAATALLSIΞAPALAHHDGHGDDDHGHAAHQHNKQDKIIΞRAQAEKAALAR I I I I I I M M I I I I I M II I I I M I I M II I I M M I I I I M I I I I M II I M I I I I II g959 MNIKHLLLTAAATALLGISAPALAHHDGHGDDDHGHAAHQHGKQDKIIΞRAQAEKAAWAR 10 20 30 40 50 60 70 80 90 100 109 m959.pep VGGKITDIDLEHDNGRPHYDVEIVKNGQEYKWVDARTGRVISSRRDDX I I M I II I I I I 1 M II I I I I I I I I I I II M I M I I I I I I M I I I I I M I g959 VGGKITDIDLEHDDGRPHYDVEIVKNGQEYKVWDARTGRVISSRRDDX 70 80 90 100 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2939>: a959.seq 1 ATGAACTTCA AACGCCTTCT CTTGACCGCC GCCGCAACCG CACTGATGGG 51 CATTTCCGCC CCCGCACTCG CCCACCACGA CGGACACGGC GATGACGACC 101 ACGGACACGC CGCACACCAA CACAGCAAAC AAGACAAAAT CATCAGCCGC 151 GCCCAAGCCG AAAAAGCAGC GTTGGCGCGT GTCGGCGGCA AAATCACCGA 201 CATCGATCTC GAACACGACA ACGGCCGTCC GCACTATGAT GTCGAAATCG 251 TCAAAAACGG ACAGGAATAC AAAGTCGTTG TCGATGCCCG TACCGGCCGC 301 GTGATTTCCT CCCGCCGCGA CGACTGA This corresponds to the amino acid sequence <SEQ ID 2940; ORF 959. a>: a959.pep 1 MΝFKRLLLTA AATALMGISA PALAHHDGHG DDDHGHAAHQ HSKQDKIISR 51 AQAEKAALAR VGGKITDIDL EHDΝGRPHYD VEIVKΝGQEY KVWDARTGR 101 VISSRRDD* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N. meningitidis ORF 959 shows 94.4% identity over a 108 aa overlap with a predicted ORF (ORF 959) from N meningitidis a959/m959 94.4% identity in 108 aa overlap 10 20 30 40 50 60 a959.pep MΝFKRLLLTAAATALMGISAPALAHHDGHGDDDHGHAAHQHΞKQDKIISRAQAEKAALAR I M M I I I I M I M M M I I I I I M I I M I I I M M I I I I M I I I M I I I I I I I I I I I M m959 MΝIKHLLLTSAATALLSISAPALAHHDGHGDDDHGHAAHQHΝKQDKIISRAQAEKAALAR 10 20 30 40 50 60 70 80 90 100 109 a959.pep VGGKITDIDLEHDΝGRPHYDVEIVKΝGQEYKWVDARTGRVISSRRDDX I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I II II I I I I I I I I I I I i m959 VGGKITDIDLEHDΝGRPHYDVEIVKΝGQEYKVWDARTGRVISSRRDDX 70 80 90 100 g960.seq not found yet g960.pep not found yet The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2941>: m960.seq 1 ATGCAAGTAA ATATTCAGAT TCCCTGTATG CTGTACAGAC GCGGGAGTGT 51 TAAGCCCCCC TTGTTTGAAG CTCCGCGGCT CCTGCCGAGC TTCACCGACC 101 CCGTTGTGCC CAAGCTCTCT GCTCCCGGCG GCTACATTGT CGACATCCGC 151 AAAGGCAATC TGAAAACCGA AATCGAAAAG CTGGCCAAAC AGCCCGAGT 201 TGCCTATCTG AAACAGCTCC AAGTAGCGAA AAACGTCAAC TGGAACCAGG 251 TGCAACTGGC TTACGATAAA TGGGACTATA AGCAGGAAGG CTTAACCAGA 301 GCCGGTGCAG CGATTATCGC GCTGGCTGTT ACCGTGGTTA CTGCGGGCGC 351 GGGAGTCGGA GCCGCACTAG GCTTAAACGG CGCAGCCGCA GCAGCGGCCG 401 ATGCCGCCTT TGCCTCACTC GCTTCTCAGG CTTCCGTATC GCTCATCAAC 451 AATAAAGGCG ATGTCGGCAA AACCCTGAAG GAACTGGGCA GAAGCCGCAG 501 GGTAAAAAAT CTGGTTGTAG CGGCGGCAAC GGCAGGCGTA TCCAACAAAC 551 TCGGTGCCTC TTCCCTTGCC ACTTGGAGCG AAACCCCTTG GGTAAACAAC 601 CTCAACGTTA ACCTGGCCAA TGCGGGCAGT GCCGCGCTGA TCAACACCGC 651 TGTTAACGGC GGCAGCCTGA AAGACAATCT GGAGGCAAAT ATCCTGGCGG 701 CATTGGTGAA TACCGCGCAT GGGGAGGCGG CGAGTAAGAT CAAAGGACTG 751 GATCAGCACT ATGTCGCCCA CAAAATCGCT CATGCCGTAG CGGGCTGTGC 801 GGCTGCAGCG GCGAATAAGG GCAAATGTCA GGACGGCGCG ATCGGTGCGG 851 CTGTGGGTGA GATTGTCGGG GAGGCTTTGG TTAAAAATAC CGATTTTAGC 901 GATATGACCC CGGAACAATT AGATCTGGAA GTTAAGAAAA TTACCGCCTA 951 TGCCAAACTT GCGGCAGGTA CAGTTGCAGG CGTAACGGGA GGAGATGTCA 1001 ATACTGCTGC ACAAACCGCA CAAAACGCGG TAGAAAATAA TGCGGTTAAA 1051 GCTGTTGTAA CTGCTGCAAA AGTGGTTTAT AAGGTAGCCA GAAAAGGATT 1101 AAAAAACGGG AAAATCAACG TTAGAGATTT AAAACAGACG TTGAAAGACG 1151 AAGGTTATAA TTTAGCCGAC AACCTGACCA CCTTATTCGA CGAAACATTG 1201 GATTGGAACG ATGCCAAAGC CGTTATTGAT ATTGTCGTCG GAACAGAGCT 1251 GAATCGCGCT AATAAAGGGG AAGCGGCACA AAAGGTCAAG GAAGTTTTAG 1301 AAAAAAATCG TCCTTATATC CCTAATAAAG GTGCTGTACC GAATATGAGT 1351 ACATACATGA AAAATAATCC TTTTGGAAAA CAGCTGGCTC AAATTTCAGA 1401 AAAGACAACG CTTCCGACGC AGCAAGGGCA GTCTGTCTTC TTGGTAAAAA 1451 GAAACCAAGG GTTATTAAAA ACCGGTGATA GGTTTTATTT AGATGGCCAA 1501 CATAAAAATC ATTTAGAGGT TTTTGATAAA AATGGGAACT TTAAGTTTGT 1551 TCTAAATATG GATGGTTCGC TTAACCAAAT GAAAACTGGG GCAGCAAAAG 1601 GTCGTAAATT AAACTTAAAA TAG This corresponds to the amino acid sequence <SEQ ID 2942; ORF 960>: m960.pep 1 MQVNIQIPCM LYRRGSVKPP LFEAPRLLPS FTDPVVPKLS APGGYIVDIP 51 KGNLKTEIEK LAKQPEYAYL KQLQVAKNVN WNQVQLAYDK WDYKQEGLTR 101 AGAAIIALAV TWTAGAGVG AALGLNGAAA AAADAAFASL ASQASVSLIN 151 NKGDVGKTLK ELGRSRTVKN LWAAATAGV SNKLGASSLA TWSETPWVNN 201 LNVNLANAGS AALINTAVNG GSLKDNLEAN ILAALVNTAH GEAASKIKGL 251 DQHYVAHKIA HAVAGCAAAA ANKGKCQDGA IGAAVGEIVG EALVKNTDFS 301 DMTPEQLDLE VKKITAYAKL AAGTVAGVTG GDVNTAAQTA QNAVENNAVK 351 AVVTAAKWY KVARKGLKNG KINVRDLKQT LKDEGYNLAD NLTTLFDETL 401 DWNDAKAVID IWGTELNRA NKGEAAQKVK EVLEKNRPYI PNKGAVPNMS 451 TYMKNNPFGK QLAQISEKTT LPTQQGQSVF LVKRNQGLLK TGDRFYLDGQ 501 HKNHLEVFDK NGNFKFVLNM DGSLNQMKTG AAKGRKLNLK * a960.seq not found yet a960.pep not found yet g961.seq not found yet g961.pep not found yet The following partial DNA sequence was identified inN. meningitidis <SEQ ID 2943>: 961.seq 1 ATGAGCATGA AACACTTTCC AGCCAAAGTA CTGACCACAG CCATCCTTGC 51 CACTTTCTGT AGCGGCGCAC TGGCAGCCAC AAGCGACGAC GATGTTAAAA 101 AAGCTGCCAC TGTGGCCATT GTTGCTGCCT ACAACAATGG CCAAGAAATC 151 AACGGTTTCA AAGCTGGAGA GACCATCTAC GACATTGGTG AAGACGGCAC 201 AATTACCCAA AAAGACGCAA CTGCAGCCGA TGTTGAAGCC GACGACTTTA 251 AAGGTCTGGG TCTGAAAAAA GTCGTGACTA ACCTGACCAA AACCGTCAAT 301 GAAAACAAAC AAAACGTCGA TGCCAAAGTA AAAGCTGCAG AATCTGAAAT 351 AGAAAAGTTA ACAACCAAGT TAGCAGACAC TGATGCCGCT TTAGCAGATA 401 CTGATGCCGC TCTGGATGAA ACCACCAACG CCTTGAATAA ATTGGGAGAA 451 AATATAACGA CATTTGCTGA AGAGACTAAG ACAAATATCG TAAAAATTGA 501 TGAAAAATTA GAAGCCGTGG CTGATACCGT CGACAAGCAT GCCGAAGCAT 551 TCAACGATAT CGCCGATTCA TTGGATGAAA CCAACACTAA GGCAGACGAA 601 GCCGTCAAAA CCGCCAATGA AGCCAAACAG ACGGCCGAAG AAACCAAACA 651 AAACGTCGAT GCCAAAGTAA AAGCTGCAGA AACTGCAGCA GGCAAAGCCG 701 AAGCTGCCGC TGGCACAGCT AATACTGCAG CCGACAAGGC CGAAGCTGTC 751 GCTGCAAAAG TTACCGACAT CAAAGCTGAT ATCGCTACGA ACAAAGCTGA 801 TATTGCTAAA AACTCAGCAC GCATCGACAG CTTGGACAAA AACGTAGCTA 851 ATCTGCGCAA AGAAACCCGC CAAGGCCTTG CAGAACAAGC CGCGCTCTCC 901 GGCCTGTTCC AACCTTACAA CGTGGGTCGG TTCAATGTAA CGGCTGCAGT 951 CGGCGGCTAC AAATCCGAAT CGGCAGTCGC CATCGGTACC GGCTTCCGCT 1001 TTACCGAAAA CTTTGCCGCC AAAGCAGGCG TGGCAGTCGG CACTTCGTCC 1051 GGTTCTTCCG CAGCCTACCA TGTCGGCGTC AATTACGAGT GGTAA This corresponds to the amino acid sequence <SEQ ID 940; ORF 2944>: m961.pep 1 MSMKHFPAKV LTTAILATFC ΞGALAATSDD DVKKAATVAI VAAYΝΝGQEI 51 NGFKAGETIY DIGEDGTITQ KDATAADVEA DDFKGLGLKK WTNLTKTVN 101 ENKQNVDAKV KAAESEIEKL TTKLADTDAA LADTDAALDE TTNALNKLGE 151 NITTFAEETK TNIVKIDEKL EAVADTVDKH AEAFNDIADS LDETNTKADE 201 AVKTANEAKQ TAEETKQNVD AKVKAAETAA GKAEAAAGTA NTAADKAEAV 251 AAKVTDIKAD IATNKADIAK NSARIDSLDK NVANLRKETR QGLAEQAALS 301 GLFQPYNVGR FNVTAAVGGY KSESAVAIGT GFRFTENFAA KAGVAVGTSS 351 GSSAAYHVGV NYEW* a961.seq not found yet a961.pep not found yet g972.seq not found yet g972.pep not found yet The following partial DNA sequence was identified in N meningitidis <SEQ ID 2945>: m972.seq 1 TTGACTAACA GGGGGGGAGC GAAATTAAAA ACCArTTCCA AGAGTAGTGA 51 ACGAATGAGT GAAGTTGAAT ATTTCTCACA CTTTATATCG GACGGAAAAG 101 GGAAGCTTTT AGAAATTCCG CAGCGAAGAG GTAAGCAAGA CggGGTTTTT 151 GTTGATTGGA TTTCATTCAC ATTCCATGAA GATACTTTAC TGAAAGTTTC 201 CGGTTGCCCT TTATTTTCTG ATGCTGAATA CATGTATGTA TTAAGCAGAA 251 AGCTGGAAGA AATTCTAGGT TTTGGCATAA CGCGCAAATG CAAATCAAGG 301 GGCAACAAAT TCTATGAATC CATGTATAGG TTAGGTTCGG ATGATGTTGA 351 TTATGGAGAG GTGCATTTCG GArGTCAGCG CAATACTGTT TTAGTTGAGT 401 TGAAAGGTAC TGGTTGCAGC GTTGCAAGTC CGGGTTGGGA GTTGAGGCTA 451 AAGCAGTTTC TCGATGATTC GATAAGGACA AGAATAACGC GAATTGACCT 501 AGCACTTGAT TTTTTTGATG GAGAGTACAC GCCGGATCAG GCGTTGTTAG 551 ATCACGATAA TGGTTTTTTT GATAACAGCA ATCAAAGGCC GAAATCTGAA 601 ACGATCGGTA CGGCTTGGCG GAATGAGGAC GGGAGCGGCA AGACATTTTA 651 TGTAGGTCGC AAGAAAAATT CTCGTTTTGT TCGTGTTTAT GAGAAAGGCA 701 GGCAGCTTGG AGATAAAGAA AGCAAATGGG TAAGGTTCGA GATCCAGTTT 751 AATTATGGAG ATATAGAAAT ACCCTTGGAT ATTTTAATAA ATCAGGGTTC 801 GTATTTCTGT GGAGCTTTTC CAATTTGTAG AAAATTTAAA AATATGCCGG 851 TTCCCGAAAG GTTTGATCAG AGAAAGAAAA AGCTTAATTT AACTTTCGAG 901 CATAAATTGC ATTACGCGAA AAACGCGGTT GGAAAACTGG TCAATTTCAT 951 GATTGAAATG GGTTTTGATA ATAGCGAAAT TGTGGAATCT TTAAAGGCAG 1001 ATTCGGGATT TCCCAAAGGA TTAGAACCTG AAAAATATGC TCTGGAAATG 1051 TTAAGGGACG GTTTGAAACA CGGTTTTATT CATGAACAGC CGGATATTGA 1101 TTTGGAAATT GAACTTGATG AATTGGGGGT TATTGCTTTT AAAAATTCTG 1151 ACAAATTCGA TAGGGAAAAA AGGCTTTTTA GTCCTGATTA TGATGTCGAG 1201 AAAGAAAGGA AATATCAGGA ATATTTAAGT AAAGTTTATC ATCAAAATGT 1251 AGATTATGAT TATTTTTAA This corresponds to the amino acid sequence <SEQ ID 2946; ORF 972>: m97 .pep 1 LTΝRGGAKLK TXSKSSERMS EVEYFSHFIS DGKGKLLEIP QRRGKQDGVF 51 VDWISFTFHE DTLLKVSGCP LFSDAEYMYV LSRKLEEILG FGITRKCKSR 101 GΝKFYESMYR LGSDDVDYGE VHFGXQRΝTV LVELKGTGCS VASPGWELRL 151 KQFLDDSIRT RITRIDLALD FFDGEYTPDQ ALLDHDΝGFF DΝSΝQRPKSE 201 TIGTAWRΝED GSGKTFYVGR KKΝSRFVRVY EKGRQLGDKE SKWVRFEIQF 251 ΝYGDIEIPLD ILIΝQGSYFC GAFPICRKFK ΝMPVPERFDQ RKKKLΝLTFE 301 HKLHYAKΝAV GKLVΝFMIEM GFDΝSEIVES LKADSGFPKG LEPEKYALEM 351 LRDGLKHGFI HEQPDIDLEI ELDELGVIAF KΝSDKFDREK RLFSPDYDVE 401 KERKYQEYLS KVYHQΝVDYD YF* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2947>: a972.seq 1 TTGACTAACA GGGGGGGAGC GAAATTAAAA ACCAATTCCA AGAGTAGTGA 51 ACGAATGAGT GAAGTTGAAT ATTTCTCACA CTTTATATCG GACGGAAAAG 101 GGAAGCTTTT AGAAATTCCG CAGCGAAGAG GTAAGCAAGA CGGGGTTTTT 151 GTTGATTGGA TTTCATTCAC ATTCCATGAA GATACTTTAC TGAAAGTTTC 201 CGGTTGCCCT TTATTTTCTG ATGCTGAATA CATGTATGTA TTAAGCAGAA 251 AGCTGGAAGA AATTCTAGGT TTTGGCATAA CGCGCAAATG CAAATCAAGG 301 GGCAACAAAT TCTATGAATC CATGTATAGG TTAGGTTCGG ATGATGTTGA 351 TTATGGAGAG GTGCATTTCG GAGGTCAGCG CAATACTGTT TTAGTTGAGT 401 TGAAAGGTAC TGGTTGCAGC GTTGCAAGTC CGGGTTGGGA GTTGAGGCTA 451 AAGCAGTTTC TCGATGATTC GATAAGGACA AGAATAACGC GAATTGACCT 501 AGCACTTGAT TTTTTTGATG GAGAGTACAC GCCGGATCAG GCGTTGTTAG 551 ATCACGATAA TGGTTTTTTT GATAACAGCA ATCAAAGGCC GAAATCTGAA 601 ACGATCGGTA CGGCTTGGCG GAATGAGGAC GGGAGCGGCA AGACATTTTA 651 TGTAGGTCGC AAGAAAAATT CTCGTTTTGT TCGTGTTTAT GAGAAAGGCA 701 GGCAGCTTGG AGATAAAGAA AGCAAATGGG TAAGGTTCGA GATCCAGTTT 751 AATTATGGAG ATATAGAAAT ACCCTTGGAT ATTTTAATAA ATCAGGGTTC 801 GTATTTCTGT GGAGCTTTTC CAATTTGTAG AAAATTTAAA AATATGCCGC 851 TTCCCGAAAG GTTTGATCAG AGAAAGAAAA CGCTTAATTT AACTTTCGAG 901 CATAAATTGC ATTACGCGAA AAACGCGGTT GGAAAACTGG TCAATTTCAT 951 GATTGAAATG GGTTTTGATA ATAGCGAAAT TGTGGAATCT TTAAAGGCAG 1001 ATTCGGGATT TCCCAAAGGA TTAGAACCTG AAAAATATGC TCTGGAAATG 1051 TTAAGGGACG GTTTGAAACA CGGTTTTATT CATGAACAGC CGGATATTGA 1101 TTTGGAAATT GAACTTGATG AATTGGGGGT TATTGCTTTT AAAAATTCTG 1151 ACAAATTCGA TAGGGAAAAA AGGCTTTTTA GTCCTGATTA TGATGTCGAG 1201 AAAGAAAGGA AATATCAGGA ATATTTAAGT AAAGTTTATC ATCAAAATGT 1251 AGATTATGAT TATTTTTAA This corresponds to the amino acid sequence <SEQ ID 2948; ORF 972. a>: a972.pep 1 LTNRGGAKLK TNSKSSERMS EVEYFSHFIS DGKGKLLEIP QRRGKQDGVF 51 VDWISFTFHE DTLLKVSGCP LFSDAEY YV LSRKLEEILG FGITRKCKSR 101 GNKFYESMYR LGSDDVDYGE VHFGGQRNTV LVELKGTGCS VASPGWELRL 151 KQFLDDSIRT RITRIDLALD FFDGEYTPDQ ALLDHDNGFF DNSNQRPKSE 201 TIGTAWRNED GSGKTFYVGR KKNSRFVRVY EKGRQLGDKE SKWVRFEIQF 251 NYGDIEIPLD ILINQGSYFC GAFPICRKFK NMPVPERFDQ RKKTLNLTFE 301 HKLHYAKNAV GKLVNFMIEM GFDNSEIVES LKADSGFPKG LEPEKYALEM 351 LRDGLKHGFI HEQPDIDLEI ELDELGVIAF KNSDKFDREK RLFSPDYDVE 401 KERKYQEYLS KVYHQNVDYD YF* m972/a972 99.3% identity in 422 aa overiap 10 20 30 40 50 60 m9 2.pep LTNRGGAKLKTXSKSSERMSEVEYFSHFISDGKGKLLEIPQRRGKQDGVFVDWISFTFHE II II II I II II II II II II I I I I I II I I I I I II I I II II I II I II II I I I I I II I II II a972 LTNRGGAKLKTNSKSSERMSEVEYFSHFISDGKGKLLEIPQRRGKQDGVFVDWISFTFHE 10 20 30 40 50 60 70 80 90 100 110 120 m972.pep DTLLKVSGCPLFSDAEYMYVLSRKLEEILGFGITRKCKSRGNKFYESMYRLGSDDVDYGE II II I II II II I II I II II II I I I I II II II I II II II II I II I II II II I II II II I II a972 DTLLKVSGCPLFSDAEYMYVLSRKLEEILGFGITRKCKSRGNKFYESMYRLGSDDVDYGE 70 80 90 100 110 120 130 140 150 160 170 180 m972. pep VHFGXQRNTVLVELKGTGCSVASPGWELRLKQFLDDSIRTRITRIDLALDFFDGEYTPDQ MM II II II II II I II II II II II II I II I II II I I II I II I II II I II I II II II II a972 VHFGGQRNTVLVELKGTGCSVASPGWELRLKQFLDDSIRTRITRIDLALDFFDGEYTPDQ 130 140 150 160 170 180 190 200 210 220 230 240 m972.pep ALLDHDNGFFDNSNQRPKSETIGTAWRNEDGΞGKTFYVGRKKNSRFVRVYEKGRQLGDKE MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a972 ALLDHDNGFFDNSNQRPKSETIGTAWRNEDGSGKTFYVGRKKNSRFVRVYEKGRQLGDKE 190 200 210 220 230 240 250 260 270 280 290 300 m972.pep SKWVRFEIQFNYGDIEIPLDILINQGSYFCGAFPICRKFKNMPVPERFDQRKKKLNLTFE I II II I I II II I II I I I I II I I I I I II I I I I II II II I II II I II I I I I II I I llllll a9 2 SKWVRFEIQFNYGDIEIPLDILINQGSYFCGAFPICRKFKNMPVPERFDQRKKTLNLTFE 250 260 270 280 290 300 310 320 330 340 350 360 m972.pep HKLHYAKNAVGKLVNFMIEMGFDNSEIVESLKADSGFPKGLEPEKYALEMLRDGLKHGFI MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a972 HKLHYAKNAVGKLVNFMIEMGFDNSEIVESLKADSGFPKGLEPEKYALEMLRDGLKHGFI 310 320 330 340 350 360 370 380 390 400 410 420 m972.pep HEQPDIDLEIELDELGVIAFKNSDKFDREKRLFSPDYDVEKERKYQEYLSKVYHQNVDYD MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a972 HEQPDIDLEIELDELGVIAFKNSDKFDREKRLFSPDYDVEKERKYQEYLSKVYHQNVDYD 370 380 390 400 410 420 m972.pep YFX I I I a972 YFX The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2949>: g973.seq 1 ATGGACGGCG CACAACCGAA AACAAATTTT TTTGAACGCC TGATTGCCCG 51 actCGCCCGC GAACCCGATT CCGCCGAAGA CGTATTAAAC CTGCTTCGGC 101 AGGCGCACGA ACAGGAAGTT TTTGATGCCG ACACACTGAC CCGGCTGGAA 151 AAAGTATTGG ACTTTGCCGA GCTGGAAGTG CGCGATGCGA TGATTACGCG 201 CAGCCGCATG AACGTATTGA AAGAAAACGA CAGCATCGAA CGCATCACCG 251 CCTACGTCAT CGATACCGCC CATTCGCGCT TCCCCGTCAT CGGCGAAGAC 301 AAAGACGAAG TTTTGGGCAT TTTGCACGCC AAAGACCTGC TCAAATATAT 351 GTTCAACCCC GAGCAGTTCC ACCTGAAATC CGTCTTGCGC CCTGCCGTTT 401 TCGTGCCCGA AGGCAAATCT TTGACCGCCC TTTTAAAAGA GTTCCGCGAA 451 CAGCGCAACC ATATGGCAAT CGTCATCGAC GAATACGGCG GCACGTCGGG 501 TTTGGTCACC TTTGAAGACA TCATCGAGCa aatcgtcggt gacaTCGAAG 551 ACGAGTTTGA CGAAGACGAA AGCGccgacg acatCCACTC cgTTTccgCC 601 GAACGCTGGC GCATCCacgc ggctaCCGAA ATCGAAGaca TCAACGCCTT 651 TTTCGGTACG GAatacggca gcgaagaagc cgacaccatc ggcggctTGG 701 TCATTCAGGA ATTGGGACAC CTGCCCGTGC GCGGCGAAAA AGTCCTTAtc 751 ggcgGTTTGC agttcaccgt CGCCCGCGCC GACAACCGCC GCCTGCACAC 801 GCTGATGGCG ACCCGCGTGA AGTAAGCAGA GCCTGCCcgc accgccgttT 851 CTGCacAGTT TAG This corresponds to the amino acid sequence <SEQ ID 2950; ORF 973.ng>: g973.pep 1 MDGAQPKTΝF FERLIARLAR EPDSAEDVLΝ LLRQAHEQEV FDADTLTRLE 51 KVLDFAELEV RDAMITRSRM ΝVLKEΝDSIE RITAYVIDTA HSRFPVIGED 101 KDEVLGILHA KDLLKYMFΝP EQFHLKSVLR PAVFVPEGKS LTALLKEFRE 151 QRΝHMAIVID EYGGTSGLVT FEDIIEQIVG DIEDEFDEDE SADDIHSVSA 201 ERWRIHAATE IEDIΝAFFGT EYGSEEADTI GGLVIQELGH LPVRGEKVLI 251 GGLQFTVARA DΝRRLHTLMA TRVK* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2951>: m973.seq 1 ATGGACGGCG CACAACCGAA AACGAATTTT TTTGAACGCC TGATTGCCCG 51 ACTCGCCCGC GAACCCGATT CCGCCGAAGA CGTATTAAAC CTGCTTCGGC 101 AGGCGCACGA GCAGGAAGTT TTTGATGCGG ATACGCTTTT AAGATTGGAA 151 AAAGTCCTCG ATTTTTCCGA TTTGGAAGTG CGCGACGCGA TGATTACGCG 201 CAGCCGTATG AACGTTTTAA AAGAAAACGA CAGCATCGAG CGCATCACCG 251 CCTACGTTAT CGATACCGCC CATTCGCGCT TCCCCGTCAT CGGCGAAGAC 301 AAAGACGAAG TTTTGGGCAT TTTGCACGCC AAAGACCTGC TCAAATATAT 351 GTTTAACCCC GAGCAGTTCC ACCTCAAATC CATTCTCCGC CCCGCCGTCT 401 TCGTCCCCGA AGGCAAATCG CTGACCGCCC TTTTAAAAGA GTTCCGCGAA 451 CAGCGCAACC ATATGGCGAT TGTCATCGAC GAATACGGCG GCACATCCGG 501 CTTGGTCACC TTTGAAGACA TCATCGAGCA AATCGTCGGC GAAATCGAAG 551 ACGAGTTTGA CGAAGACGAT AGCGCCGACA ATATCCATGC CGTTTCTTC 601 GaACGcTGGC GCATCCATGC AGCTACCGAA ATCGAAGACA TCAACACCTT 651 CTTCGGCACG GAATACAGCA kCGAAGAAGC CGACACCATT GGCGGCCTGG 701 TCATTCAAGA GTTGGGACAT CTGCCCGTGC GCGGCGAAAA AGTCCTTATC 751 GGCGGTTTGC AGTTCACCGT CGCACGCGCC GACAACCGCC GCCTGCATAC 801 GCTGATGGCG ACCCGCGTGA AGTAA This corresponds to the amino acid sequence <SEQ ID 2952; ORF 973>: m973.pep 1 MDGAQPKTNF FERLIARLAR EPDSAEDVLN LLRQAHEQEV FDADTLLRLE 51 KVLDFSDLEV RDAMITRSRM NVLKENDSIE RITAYVIDTA HSRFPVIGED 101 KDEVLGILHA KDLLKYMFNP EQFHLKSILR PAVFVPEGKS LTALLKEFRE 151 QRNHMAIVID EYGGTSGLVT FEDIIEQIVG EIEDEFDEDD SADNIHAVSS 201 ERWRIHAATE IEDINTFFGT EYSXEEADTI GGLVIQELGH LPVRGEKVLI 251 GGLQFTVARA DNRRLHTLMA TRVK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 973 shows 95.6% identity over a 274 aa overlap with a predicted ORF (ORF 973. ng) from N gonorrhoeae: m973/g973 10 20 30 40 50 60 m973. pep MDGAQPKTΝFFERLIARLAREPDSAEDVLΝLLRQAHEQEVFDADTLLRLEKVLDFSDLEV I I I I M I M M g973 MDGAQPKTΝFFERLIARLAREPDSAEDVLΝLLRQAHEQEVFDADTLTRLEKVLDFAELEV 10 20 30 40 50 60 70 80 90 100 110 120 m973.pep RDAMITRSRMΝVLKEΝDSIERITAYVIDTAHSRFPVIGEDKDEVLGILHAKDLLKY FΝP g973 RDAMITRSRMΝVLKEΝDSIERITAYVIDTAHSRFPVIGEDKDEVLGILHAKDLLKYMFΝP 70 80 90 100 110 120 130 140 150 160 170 180 m973. pep EQFHLKSILRPAVFVPEGKSLTALLKEFREQRΝHMAIVIDEYGGTSGLVTFEDIIEQIVG g973 EQFHLKSVLRPAVFVPEGKSLTALLKEFREQRΝHMAIVIDEYGGTSGLVTFEDIIEQIVG 130 140 150 160 170 180 190 200 210 220 230 240 m973.pep EIEDEFDEDDSADΝIHAVSSERWRIHAATEIEDIΝTFFGTEYSXEEADTIGGLVIQELGH 1 1 1 1 1 1 1 1 M 1 1 1 I I I g973 DIEDEFDEDESADDIHSVSAERWRIHAATEIEDIΝAFFGTEYGSEEADTIGGLVIQELGH 190 200 210 220 230 240 250 260 270 m973.pep LPVRGEKVLIGGLQFTVARADΝRRLHTLMATRVKX g973 LPVRGEKVLIGGLQFTVARADΝRRLHTLMATRVKX 250 260 270 The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 2953>: a973. seq 1 ATGGACGGCG CACAACCGAA AACAAATTTT TTTGAACGCC TGATTGCCCG 51 ACTCGCCCGC GAACCCGATT CCGCCGAAGA CGTATTGACC CTGTTGCGCC 101 AAGCGCACGA ACAGGAAGTA TTTGATGCGG ATACGCTTTT AAGATTGGAA 151 AAAGTCCTCG ATTTTTCTGA TTTGGAAGTG CGCGACGCGA TGATTACGCG 201 CAGCCGTATG AACGTTTTAA AAGAAAACGA CAGCATCGAA CGCATCACCG 251 CCTACGTTAT CGATACCGCC CATTCGCGCT TCCCCGTCAT CGGTGAAGAC 301 AAAGACGAAG TTTTGGGTAT TTTGCACGCC AAAGACCTGC TCAAATATAT 351 GTTCAACCCC GAGCAGTTCC ACCTCAAATC GATATTGCGC CCTGCCGTCT 401 TCGTCCCCGA AGGCAAATCG CTGACCGCCC TTTTAAAAGA GTTCCGCGAA 451 CAGCGCAACC ATATGGCAAT CGTCATCGAC GAATACGGCG GCACGTCGGG 501 TTTGGTAACT TTTGAAGACA TCATCGAGCA AATCGTCGGC GACATCGAAG 551 ATGAGTTTGA CGAAGACGAA AGCGCGGACA ACATCCACGC CGTTTCCGCC 601 GAACGCTGGC GCATCCACGC GGCTACCGAA ATCGAAGACA TCAACGCCTT 651 TTTCGGCACG GAATACAGCA GCGAAGAAGC CGACACCATC GGCGGCCTGG 701 TCATTCAGGA ATTGGGACAC CTGCCCGTGC GCGGCGAAAA AGTCCTTATC 751 GGCGGTTTGC AGTTCACCGT CGCCCGCGCC GACAACCGCC GCCTGCATAC 801 GCTGATGGCG ACCCGCGTGA AGTAA This corresponds to the amino acid sequence <SEQ ID 2954; ORF 973. a>: a973.pep 1 DGAQPKTNF FERLIARLAR EPDSAEDVLT LLRQAHEQEV FDADTLLRLE 51 KVLDFSDLEV RDAMITRSRM NVLKENDSIE RITAYVIDTA HSRFPVIGED 101 KDEVLGILHA KDLLKYMFNP EQFHLKSILR PAVFVPEGKS LTALLKEFRE 151 QRNHMAIVID EYGGTSGLVT FEDIIEQIVG DIEDEFDEDE SADNIHAVSA 201 ERWRIHAATE IEDINAFFGT EYSSEEADTI GGLVIQELGH LPVRGEKVLI 251 GGLQFTVARA DNRRLHTLMA TRVK* m973/a973 97.8% identity in 274 aa overlap 10 20 30 40 50 60 m973.pep MDGAQPKTNFFERLIARLAREPDSAEDVLNLLRQAHEQEVFDADTLLRLEKVLDFSDLEV II II I I II II I II II II II I II II II II I M II I I II II I I I I II II I I II I II II II II a973 MDGAQPKTNFFERLIARLAREPDSAEDVLTLLRQAHEQEVFDADTLLRLEKVLDFSDLEV 10 20 30 40 50 60 70 80 90 100 110 120 m973.pep RDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKDEVLGILHAKDLLKYMFNP I II II I II I II II II II II II II II I I II II II II II II I II II I II I I I I II II II I II a973 RDAMITRSR NVLKENDSIERITAYVIDTAHSRFPVIGEDKDEVLGILHAKDLLKYMFNP 70 80 90 100 110 120 130 140 150 160 170 180 m973. pep EQFHLKSILRPAVFVPEGKSLTALLKEFREQRNHMAIVIDEYGGTSGLVTFEDIIEQIVG II II II II II I I II II II I II II I II II II II II II I II I I II II I I I II I II II I II II a973 EQFHLKSILRPAVFVPEGKSLTALLKEFREQRNHMAIVIDEYGGTSGLVTFEDIIEQIVG 130 140 150 160 170 180 190 200 210 220 230 240 m973.pep EIEDEFDEDDSADNIHAVSSERWRIHAATEIEDINTFFGTEYSXEEADTIGGLVIQELGH : II II I II I : I II II II I I : II II II II I II II II : I II I II I I II II II I I II II I II a973 DIEDEFDEDESADNIHAVSAERWRIHAATEIEDINAFFGTEYSSEEADTIGGLVIQELGH 190 200 210 220 230 240 250 260 270 m973. pep LPVRGEKVLIGGLQFTVARADNRRLHTLMATRVKX I II I II II I II II I I II I II II II II II II II I II a973 LPVRGEKVLIGGLQFTVARADNRRLHTLMATRVKX 250 260 270 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2955>: g981.seq 1 ATGAAAAAAT GGATTGCCGC CGCCCTTGCC TGTTCCGCAC TCGCGCTGTC 51 TGCCTGCGGC GGTCAGGGCA AAGATGCCGC CGCGCCTGCC GCCAACCCCG 101 GCAAAGTGTA CCGCGTGGCT TCCAACGCCG AGTTTGCCCC CTTTGAATCT 151 TTAGACTCGA AAGGCAATGT CGAAGGTTTC GACGTGGATT TGATGAACGC 201 GATGGCGAAG GCGGGCAATT TTAAAATCGA ATTCAAACAC CAGCCGTGGG 251 ACAGCCTTTT CCCCGCCTTG AACAACGGCG ATGCGGACGT TGTGATGTCG 301 GGCGTAACCA TTACCGACGA CCGCAAACAG TCTATGGATT TCAGCGACCC 351 GTATTTTGAA ATCACCCAAG TCGTCCTCGT TCCGAAAGGC AAAAAAGTAT 401 CTTCTTCCGA AGATTTGAAA AAGATGAACA AAGTCGGCGT GGTTACCGGC 451 CACACGGGCG ATTTCTCCGT TTCCAAACTC TTGGGCAACG ACAATCCGAA 501 AATCGCGCGC TTCGAAAACG TCCCCCTGAT TATCAAAGAA CTGGAAAACG S51 GCGGCTTGGA TTCCGTGGTC AGCGACAGCG CGGTCATCGC CAATTATGTG 601 AAAAACAACC CGGCCAAAGG AATGGACTTC GTTACCCTGC CCGACTTCAC 651 CACCGAACAC TACGGCATCG CGGTACGCAA AGGCGACGAA GCAACCGTCA 701 AAATGCTGAA CGATGCGTTG GAAAAAGTAC GCGAAAGCGG CGAATACGAC 751 AAGATCTACG CCAAATATTT TGCCAAAGAG GGCGGACAGG CTGCGAAATA 801 A This corresponds to the amino acid sequence <SEQ ID 2956; ORF 981.ng>: g981.pep 1 MKKWIAAALA CSALALSACG GQGKDAAAPA ANPGKVYRVA SNAEFAPFES 51 LDSKGNVEGF DVDLMNAMAK AGNFKIEFKH QPWDSLFPAL NNGDADWMS 101 GVTITDDRKQ SMDFSDPYFE ITQ LVPKG KKVSSSEDLK KMNKVGWTG 151 HTGDFSVSKL LGNDNPKIAR FENVPLIIKE LENGGLDS SDSAVIANYV 201 KNNPAKGMDF VTLPDFTTEH YGIAVRKGDE ATVKMLNDAL EKVRESGEYD 251 KIYAKYFAKE GGQAAK* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2957>: m981.seq 1 ATGAAAAAAT GGATTGCCGC CGCCCTTGCC TGTTCCGCGC TCGCGCTGTC 51 TGCCTGCGGC GGTCAGGGCA AAGATACCGC CGCGCCTGCC GCCAACCCCG 101 ACAAAGTGTA CCGCGTGGCT TCCAACGCCG AGTTTGCCCC CTTTGAATCT 151 TTAGACTCGA AAGGCAATGT CGAAGGTTTC GATGTGGATT TGATGAACGC 201 GATGGCGAAG GCGGGCAATT TTAAAATCGA ATTCAAACAC CAGCCGTGGG 251 ACAGCCTTTT CCCCGCCTTA AACAACGGCG ATGCGGACGT TGTGATGTCG 301 GGCGTAACCA TTACCGACGA CCGCAAACAG TCTATGGACT TCAGCGACCC 351 GTATTTTGAA ATCACCCAAG TCGTCCTCGT TCCGAAAGGC AAAAAAGTAT 401 CTTCTTCCGA AGATTTGAAA AACATGAACA AAGTCGGCGT GGTAACCGGC 451 TACACGGGCG ATTTCTCCGT ATCCAAACTC TTGGGCAACG ACAATCCGAA 501 AATCGCGCGC TTTGAAAACG TTCCCCTGAT TATCAAAGAA CTGGAAAACG 551 GCGGCTTGGA TTCCGTGGTC AGCGACAGCG CGGTCATCGC CAATTATGTG 601 AAAAACAATC CGGCCAAAGG GATGGACTTC GTTACCCTGC CCGACTTCAC 651 CACCGAACAC TACGGCATCG CGGTACGCAA AGGCGACGAA GCAACCGTCA 701 AAATGCTGAA CGATGCGTTG GAAAAAGTAC GCGAAAGCGG CGAATACGAC 751 AAGATTTACG CCAAATATTT TGCAAAAGAA GACGGACAGG CCGCAAAATA 801 A This corresponds to the amino acid sequence <SEQ ID 2958; ORF 981>: m981.pep 1 MKKWIAAALA CSALALSACG GQGKDTAAPA AΝPDKVYRVA SNAEFAPFES 51 LDSKGNVEGF DVDLMNAMAK AGNFKIEFKH QPWDSLFPAL NNGDADWMS 101 GVTITDDRKQ SMDFSDPYFE ITQWLVPKG KKVSSSEDLK NMNKVGWTG 151 YTGDFSVSKL LGNDNPKIAR FENVPLIIKE LENGGLDSW SDSAVIANYV 201 KNNPAKGMDF VTLPDFTTEH YGIAVRKGDE ATVKMLNDAL EKVRESGEYD 251 KIYAKYFAKE DGQAAK* 981/g981 98.1% identity in 266 aa overlap 10 20 30 40 50 60 981.pep MKKWIAAALACSALALSACGGQGKDTAAPAANPDKVYRVASNAEFAPFESLDSKGNVEGF I I I I I M I I I M M I M I I I M I I M I I I I I M 1 1 1 1 1 1 1 1 1 1 I I I I 1 1 1 1 I I 1 1 1 1 1 1 g981 MKKWIAAALACSALALSACGGQGKDAAAPAANPGKVYRVAΞNAEFAPFESLDSKGNVEGF 10 20 30 40 50 60 70 80 90 100 110 120 981 . pep DVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADWMSGVTITDDRKQSMDFΞDPYFE g981 DVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADWMSGVTITDDRKQSMDFSDPYFE 70 80 90 100 110 120 130 140 150 160 170 180 981.pep ITQWLVPKGKKVSSSEDLKNMNKVGWTGYTGDFSVSKLLGNDNPKIARFENVPLIIKE g981 ITQWLVPKGKKVSΞSEDLKKMNKVGWTGHTGDFSVΞKLLGNDNPKIARFENVPLI IKE 130 140 150 160 170 180 190 200 210 220 230 240 981 . pep LENGGLDSWSDSAVIANYVKNNPAKGMDFVTLPDFTTEHYGI AVRKGDEATVKMLNDAL g981 LENGGLDS WSDSAVIANYVKNNPAKGMDFVTLPDFTTEHYGIAVRKGDEATVKMLNDAL 190 200 210 220 230 240 250 260 981 . pep EKVRESGEYDKIYAKYFAKEDGQAAKX g981 EKVRESGEYDKIYAKYFAKEGGQAAKX 250 260 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2959>: a981.seq 1 ATGAAAAAAT GGATTGCCGC CGCCCTTGCC TGTTCCGCGC TCGCGCTGTC 51 TGCCTGCGGC GGTCAGGGTA AAGATGCCGC CGCGCCCGCC GCAAATCCCG 101 ACAAAGTGTA CCGCGTGGCT TCCAACGCCG AGTTTGCCCC CTTTGAATCT 151 TTAGACTCGA AAGGCAATGT CGAAGGTTTC GATGTGGATT TGATGAACGC 201 GATGGCGAAG GCGGGCAATT TTAAAATCGA ATTCAAACAC CAGCCGTGGG 251 ACAGCCTTTT CCCCGCCTTG AACAACGGCG ATGCGGACGT TGTGATGTCG 301 GGCGTAACCA TTACCGACGA CCGCAAACAG TCTATGGACT TCAGCGACCC 351 GTATTTTGAA ATCACCCAAG TCGTCCTCGT TCCGAAAGGC AAAAAAATAT 401 CTTCTTCCGA AGATTTGAAA AACATGAACA AAGTCGGCGT GGTAACCGGC 451 TACACGGGCG ATTTCTCCGT ATCCAAACTC TTGGGCAACG ACAACCCGAA 501 AATCGCGCGC TTTGAAAACG TTCCCCTGAT TATCAAAGAA CTGGAAAACG 551 GCGGCTTGGA TTCCGTGGTC AGCGACAGCG CAGTCATCGC CAATTATGTG 601 AAAAACAATC CGACCAAAGG GATGGACTTC GTTACCCTGC CCGACTTCAC 651 CACCGAACAC TACGGCATCG CGGTACGCAA AGGCGACGAA GCAACCGTCA 701 AAATGCTGAA CGATGCGTTG AAAAAAGTAC GCGAAAGCGG CGAATACGAC 751 AAAATCTACG CCAAATATTT TGCAAAAGAA GACGGACAGG CCGCAAAATA 801 A This corresponds to the amino acid sequence <SEQ ID 2960; ORF 981.a>: a981.pep 1 MKKWIAAALA CSALALSACG GQGKDAAAPA AΝPDKVYRVA SNAEFAPFES 51 LDSKGNVEGF DVDLMNAMAK AGNFKIEFKH QPWDSLFPAL NNGDADWMS 101 GVTITDDRKQ SMDFSDPYFE ITQWLVPKG KKISSSEDLK NMNKVGWTG 151 YTGDFSVSKL LGNDNPKIAR FENVPLIIKE LENGGLDSW SDSAVIANYV 201 KNNPTKGMDF VTLPDFTTEH YGIAVRKGDE ATVKMLNDAL KKVRESGEYD 251 KIYAKYFAKE DGQAAK* m98 l/a981 98.5% identity in 266 aa overlap 10 20 30 40 50 60 m981. pep MKKWIAAALACSALALSACGGQGKDTAAPAANPDKVYRVASNAEFAPFESLDSKGNVEGF II II II I II I II II II II II II II M I II I II II I II II I II I I II I I I II II II II II I a981 MKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASNAEFAPFESLDSKGNVEGF 10 20 30 40 50 60 70 80 90 100 110 120 m981. pep DVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADWMSGVTITDDRKQSMDFSDPYFE II II II II I II II II II I II I II I II I II II II II I I II II II II II I I II II II II II I a981 DVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADWMSGVTITDDRKQSMDFSDPYFE 70 80 90 100 110 120 130 140 150 160 170 180 m981.pep ITQWLVPKGKKVSSSEDLKNMNKVGWTGYTGDFSVSKLLGNDNPKIARFENVPLIIKE II I II II II II M II II I II II II I II I II II II I II II II I II II II II II I II II II I a981 ITQWLVPKGKKISSSEDLKNMNKVGWTGYTGDFSVSKLLGNDNPKIARFENVPLIIKE 130 140 150 160 170 180 190 200 210 220 230 240 m981.pep LENGGLDSWSDSAVIANYVKNNPAKGMDFVTLPDFTTEHYGIAVRKGDEATVKMLNDAL II II I II II II II II I II II II II : I II I II II II II II II I II I I II II II II II I II I a981 LENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDFTTEHYGIAVRKGDEATVKMLNDAL 190 200 210 220 230 240 250 260 m981. pep EKVRESGEYDKIYAKYFAKEDGQAAKX M II I I I I I I II I II I II II I II II I I a981 KKVRESGEYDKIYAKYFAKEDGQAAKX 250 260 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2961>: g 982. seq 1 atcgcatcgc aaaaccttcg attcgacaat cgattcctcc aaaaaatggt 51 caacggcgTg aatattttgc cggccgcCga ttgggtagcC ttgGGcgcCA 101 AAGGCCGCAA CGTGGTGGTT GACCGCGCTT TCGGCGGCCC GCACATCACC 151 AAAGACGGCG TAACCGTCGC CAAAGAAATC GAACTGAAAG ACAAGTTTGA 201 AAATATGGGC GCGCAAATGG TAAAAGAAGT CGCGTCCAAA ACCAAcgaCg 251 tagCCGgcga cggtacgact accgCCACCG TATTGGCACA ATCCATCGTT 301 GCCGAAggcA TGAAATACGT TACCGCCGGC ATGAACCCGA CCGATCTGAA 351 ACGCGGCATC GACAAAGccg ttgCCGCTtt ggttgAAGAg CTGAAAAACA 401 TCGCCAAACC TTGCGATACT TCCAAAGAAA TCGCCCAAGT CGGCTCGATT 451 TCCGCCAACT CCGACGAACA AGtcgGCGCG ATTATCGCCG AAGCGATGGA 501 AAAAGTCGGC AAAGAAGgcg tgattacCGT TGAAGACGGC AAATCTTTGG 551 AAAACGAGCT GGACGTGGTT GAAGGTATGC AGTTCGACCG CGGCTACCTG 601 TCCCCTTACT TTATCAACGA CGCGGAAAAA CAAATCGCCG GTCTGGACAA 651 TCCGTTTGTT TTGCTGTTCG ACAAAAAAAT CAGCAACATC CGCGACCTGC 701 TGCCCGTGTT GGAACAAGTG GCGAAAGCCA GCCGCCCGCT GTTGATTATC 751 GCTGAAGACG TAGAAGGCGA AGCCTTGGCG ACTTTGGTCG TGAACAACAT 801 CCGCGGCATC CTGAAAACCG TTGCCGTCAA AGCccccggc tTCGGcGACC 851 GCCGCAAAGC GATgctgcaa gaCATCGCCA TCCTGACcgg cggcgTagtG 901 ATTtccGAAG Aagtcggcct GTCTTTGGAA AAAgcgactT TGgacgaCTT 951 Gggtcaaacc aaACGcatCG AAATCGGtga agaaaacact ACCGTCATcg 1001 acgGCTTCGG CGACGcagcC CAAAtcgaag cgCGTGTTGC CGAAATCCGC 1051 CAACAAATCG AAACCGCGAC CAGCGATTAC GACAAAGAAA AACTGCAAGA 1101 GCGCGTTGCC AAACTGGCAG GAGGCGTGGC AGTGATCAAA GTCGGCGCGG 1151 CGACCGAAGT CGAAATGAAA GAGAAAAAAG ACCGCGTGGA AGACGCGCTG 1201 CACGCTACCC GCGCAGCCGT TGAAGAAGGC GTGGTTGCAG GCGGCGGCGT 1251 AGCCCTGTTG CGCGCCCGTG CCGCTTTGGA AAACCTGCAC ACCGGCAATG 1301 CCGACCAAGA CGCAGGCGTA CAAATCGTAT TGCGCGCCGT TGAGTCTCCG 1351 CTGCGCCAAA TCGTTGCCAA CGCAGGCGGA GAACCCAGCG TGGTGGTGAA 1401 CAAAGTGTTG GAAGGCAAAG GCAactacgG TTACAACGCa ggctcCGGCG 1451 AATACGgcga CATGATCGGA ATGGGCGTAC TCGACCCTGC CAAAGTAACC 1501 CGTTCCGCGC TGCAACACGC CGCGTCTAtC GCCGGTCTGA TGCTGACGAC 1551 CGACTGCATG ATTGCCGAAA TCCCTGAAGA AAAACCGGCT GTGCCCGATA 1601 TGGGGGGAAT GGGCGGTATG GGCGGCATGA TGTAA This corresponds to the amino acid sequence <SEQ ID 2962; ORF 982.ng>: g982.pep 1 IASQΝLRFDΝ RFLQKMVΝGV ΝILPAADWVA LGAKGRΝWV DRAFGGPHIT 51 KDGVTVAKEI ELKDKFEΝMG AQMVKEVASK TΝDVAGDGTT TATVLAQSIV 101 AEGMKYVTAG MΝPTDLKRGI DKAVAALVEE LKΝIAKPCDT SKEIAQVGSI 151 SAΝSDEQVGA IIAEAMEKVG KEGVITVEDG KSLEΝELDW EGMQFDRGYL 201 SPYFIΝDAEK QIAGLDΝPFV LLFDKKISΝI RDLLPVLEQV AKASRPLLII 251 AEDVEGEALA TLVVΝΝIRGI LKTVAVKAPG FGDRRKAMLQ DIAILTGGW 301 ISEEVGLSLE KATLDDLGQT KRIEIGEEΝT TVIDGFGDAA QIEARVAEIR 351 QQIETATSDY DKEKLQERVA KLAGGVAVIK VGAATEVEMK EKKDRVEDAL 401 HATRAAVEEG VVAGGGVALL RARAALEΝLH TGΝADQDAGV QIVLRAVESP 451 LRQIVAΝAGG EPSVWΝKVL EGKGΝYGYΝA GSGEYGDMIG MGVLDPAKVT 501 RSALQHAASI AGLMLTTDCM IAEIPEEKPA VPDMGGMGGM GGMM* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2963>: m982.seq 1 ATGGCAGCAA AAGACGTACA GTTCGGCAAT GAAGTCCGTC AAAAAATGGT 51 AAACGGCGTG AACATTCTGG CAAACGCCGT CCGCGTAACC TTGGGCCCCA 101 AAGGTCGCAA CGTAGTCGTT GACCGCGCAT TCGGCGGCCC GCACATCACC 151 AAAGACGGCG TAACCGTCGC CAAAGAAATC GAACTGAAAG ACAAGTTTGA 201 AAATATGGGC GCGCAAATGG TGAAAGAAGT TGCGTCCAAA ACCAACGACG 251 TGGCAGGCGA CGGTACGACT ACCGCCACCG TACTGGCGCA ATCCATCGTT 301 GCCGAAGGTA TGAAATATGT TACCGCAGGT ATGAATCCGA CCGACCTGAA 351 ACGCGGTATC GATAAAGCCG TCGCCGCTTT GGTTGACGAA CTGAAAAACA 401 TCGCCAAACC TTGCGACACT TCTAAAGAAA TCGCCCAAGT CGGCTCTATT 451 TCCGCCAACT CCGACGAACA AGTCGGCGCG ATTATCGCCG AAGCGATGGA 501 AAAAGTCGGC AAAGAAGGCG TGATTACCGT TGAAGACGGC AAGTCTTTGG 551 AAAACGAGCT GGACGTAGTT GAAGGTATGC AGTTCGACCG CGGCTACCTG 601 TCTCCTTACT TCATCAACGA TGCGGAAAAA CAAATCGCTG CTTTGGACAA 651 TCCGTTTGTA TTGTTGTTCG ACAAAAAAAT CAGCAACATC CGCGACCTGC 701 TGCCTGTTTT GGAACAAGTG GCAAAAGCCA GCCGTCCGCT GTTGATTATC 751 GCTGAAGACG TAGAAGGCGA AGCCTTGGCG ACTTTGGTCG TGAACAACAT 801 CCGAGGCATC CTGAAAACCG TTGCCGTCAA AGCCCCTGGC TTCGGCGACC 851 GCCGCAAAGC GATGTTGCAA GACATCGCCA TCCTGACCGG CGGCGTGGTG 901 ATTTCCGAAG AAGTCGGTCT GTCTTTGGAA AAAGCGACTT TGGACGACTT 951 GGGTCAAGCC AAACGCATCG AAATCGGTAA AGAAAACACC ACCATCATCG 1001 ACGGCTTTGG CGACGCAGCC CAAATCGAAG CGCGTGTTGC CGAAATCCGC 1051 CAACAAATCG AAACCGCAAC CAGCGATTAC GACAAAGAAA AACTGCAAGA 1101 GCGCGTGGCT AAATTGGCAG GCGGCGTGGC AGTCATCAAA GTCGGTGCCG 1151 CGACCGAAGT CGAAATGAAA GAGAAAAAAG ACCGCGTGGA AGACGCGCTG 1201 CACGCTACCC GCGCAGCCGT TGAAGAAGGC GTGGTTGCAG GCGGCGGCGT 1251 AGCCCTGTTG CGTGCCCGTG CTGCTTTGGA AAACCTGCAC ACCGGCAATG 1301 CCGACCAAGA CGCAGGCGTA CAAATCGTCT TGCGCGCCGT TGAGTCTCCG 1351 CTGCGCCAAA TCGTTGCCAA CGCAGGCGGC GAACCCAGCG TGGTTGTGAA 1401 CAAAGTATTG GAAGGCAAAG GCAACTACGG TTACAACGCT GGCAGCGGCG 1451 AATACGGCGA TATGATCGAA ATGGGCGTAC TCGACCCCGC CAAAGTAACC 1501 CGTTCTGCGC TGCAACACGC CGCATCTATC GCCGGCTTGA TGCTGACCAC 1551 TGATTGCATG ATCGCTGAAA TCCCCGAAGA CAAACCGGCT GTGCCTGATA 1601 TGGGCGGCAT GGGTGGTATG GGCGGCATGA TGTAA This corresponds to the amino acid sequence <SEQ ID 2964; ORF 982>: m982.seq 1 ATGGCAGCAA AAGACGTACA GTTCGGCAAT GAAGTCCGTC AAAAAATGGT 51 AAACGGCGTG AACATTCTGG CAAACGCCGT CCGCGTAACC TTGGGCCCCA 101 AAGGTCGCAA CGTAGTCGTT GACCGCGCAT TCGGCGGCCC GCACATCACC 151 AAAGACGGCG TAACCGTCGC CAAAGAAATC GAACTGAAAG ACAAGTTTGA 201 AAATATGGGC GCGCAAATGG TGAAAGAAGT TGCGTCCAAA ACCAACGACG 251 TGGCAGGCGA CGGTACGACT ACCGCCACCG TACTGGCGCA ATCCATCGTT 301 GCCGAAGGTA TGAAATATGT TACCGCAGGT ATGAATCCGA CCGACCTGAA 351 ACGCGGTATC GATAAAGCCG TCGCCGCTTT GGTTGACGAA CTGAAAAACA 401 TCGCCAAACC TTGCGACACT TCTAAAGAAA TCGCCCAAGT CGGCTCTATT 451 TCCGCCAACT CCGACGAACA AGTCGGCGCG ATTATCGCCG AAGCGATGGA 501 AAAAGTCGGC AAAGAAGGCG TGATTACCGT TGAAGACGGC AAGTCTTTGG 551 AAAACGAGCT GGACGTAGTT GAAGGTATGC AGTTCGACCG CGGCTACCTG 601 TCTCCTTACT TCATCAACGA TGCGGAAAAA CAAATCGCTG CTTTGGACAA 651 TCCGTTTGTA TTGTTGTTCG ACAAAAAAAT CAGCAACATC CGCGACCTGC 701 TGCCTGTTTT GGAACAAGTG GCAAAAGCCA GCCGTCCGCT GTTGATTATC 751 GCTGAAGACG TAGAAGGCGA AGCCTTGGCG ACTTTGGTCG TGAACAACAT 801 CCGAGGCATC CTGAAAACCG TTGCCGTCAA AGCCCCTGGC TTCGGCGACC 851 GCCGCAAAGC GATGTTGCAA GACATCGCCA TCCTGACCGG CGGCGTGGTG 901 ATTTCCGAAG AAGTCGGTCT GTCTTTGGAA AAAGCGACTT TGGACGACTT 951 GGGTCAAGCC AAACGCATCG AAATCGGTAA AGAAAACACC ACCATCATCG 1001 ACGGCTTTGG CGACGCAGCC CAAATCGAAG CGCGTGTTGC CGAAATCCGC 1051 CAACAAATCG AAACCGCAAC CAGCGATTAC GACAAAGAAA AACTGCAAGA 1101 GCGCGTGGCT AAATTGGCAG GCGGCGTGGC AGTCATCAAA GTCGGTGCCG 1151 CGACCGAAGT CGAAATGAAA GAGAAAAAAG ACCGCGTGGA AGACGCGCTG 1201 CACGCTACCC GCGCAGCCGT TGAAGAAGGC GTGGTTGCAG GCGGCGGCGT 1251 AGCCCTGTTG CGTGCCCGTG CTGCTTTGGA AAACCTGCAC ACCGGCAATG 1301 CCGACCAAGA CGCAGGCGTA CAAATCGTCT TGCGCGCCGT TGAGTCTCCG 1351 CTGCGCCAAA TCGTTGCCAA CGCAGGCGGC GAACCCAGCG TGGTTGTGAA 1401 CAAAGTATTG GAAGGCAAAG GCAACTACGG TTACAACGCT GGCAGCGGCG 1451 AATACGGCGA TATGATCGAA ATGGGCGTAC TCGACCCCGC CAAAGTAACC 1501 CGTTCTGCGC TGCAACACGC CGCATCTATC GCCGGCTTGA TGCTGACCAC 1551 TGATTGCATG ATCGCTGAAA TCCCCGAAGA CAAACCGGCT GTGCCTGATA 1601 TGGGCGGCAT GGGTGGTATG GGCGGCATGA TGTAA Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m982/g982 95.8% identity in 544 aa overlap 10 20 30 40 50 60 m982. pep MAAKDVQFGΝEVRQKMVΝGVΝILAΝAVRVTLGPKGRΝ VDRAFGGPHITKDGVTVAKEI M::: : M M I II II II I II I MM I II I I I I M II I II II M I I I M I I II g982 IASQNLRFDNRFLQKMVNGVNILPAADWVALGAKGRNVWDRAFGGPHITKDGVTVAKEI 10 20 30 40 50 60 70 80 90 100 110 120 m982. ,pep ELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAEGMKYVTAGMNPTDLKRGI II I I II II II II II II II I II II I II II I II II I I I I II I I I II I I I I I I II I II II II I g982 ELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAEGMKYVTAGMNPTDLKRGI 70 80 90 100 110 120 130 140 150 160 170 180 m982. • pep DKAVAALVDELKNIAKPCDTSKEIAQVGSISANSDEQVGAIIAEAMEKVGKEGVITVEDG II I II I I I : II II II II II II II II I II II II I I I I II II II II I I I I I II I II I II II I g982 DKAVAALVEELKNIAKPCDTSKEIAQVGSISANSDEQVGAIIAEAMEKVGKEGVITVEDG 130 140 150 160 170 180 190 200 210 220 230 240 m982. ,pep KSLENELDWEGMQFDRGYLSPYFINDAEKQIAALDNPFVLLFDKKISNIRDLLPVLEQV I II I I II II II II II II II II II II II II II I M II I II II I II I II II II II I II II I I g982 KSLENELDVVEGMQFDRGYLSPYFINDAEKQIAGLDNPFVLLFDKKISNIRDLLPVLEQV 190 200 210 220 230 240 250 260 270 280 290 300 m982. pep AKASRPLLIIAEDVEGEALATLWNNIRGILKTVAVKAPGFGDRRKAMLQDIAILTGGVV II I II I II I I I II II II II II II II II I II I I II II II II I I I II II I I I II II I II I I I g982 AKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAVKAPGFGDRRKAMLQDIAILTGGVV 250 260 270 280 290 300 310 320 330 340 350 360 m982.pep ISEEVGLSLEKATLDDLGQAKRIEIGKENTTIIDGFGDAAQIEARVAEIRQQIETATSDY I II I II II II I I II II II I M II II I M II I : I II II I I II I II I II I II II II II I I II g982 ISEEVGLSLEKATLDDLGQTKRIEIGEENTTVIDGFGDAAQIEARVAEIRQQIETATSDY 310 320 330 340 350 360 370 380 390 400 410 420 m982. pep DKEKLQERVAKLAGGVAVIKVGAATEVEMKEKKDRVEDALHATRAAVEEGVVAGGGVALL I I I II I II II II II II II II II II II I I II II II II I II II I II I II I II II II II I II I g982 DKEKLQERVAKLAGGVAVIKVGAATEVEMKEKKDRVEDALHATRAAVEEGVVAGGGVALL 370 380 390 400 410 420 430 440 450 460 470 480 m982. pep RARAALENLHTGNADQDAGVQIVLRAVESPLRQIVANAGGEPSVVVNKVLEGKGNYGYNA I II I II I I I I II II II II I II I I II II II II I II II I II II I II I II I II II II II II I I g982 RARAALENLHTGNADQDAGVQIVLRAVESPLRQIVANAGGEPSVVVNKVLEGKGNYGYNA 430 440 450 460 470 480 490 500 510 520 530 540 m982. pep GSGEYGDMIEMGVLDPAKVTRSALQHAASIAGLMLTTDCMIAEIPEDKPAVPDMGGMGGM lllllllll I II II II II II II II I I I II II I II II II II I I I I I : I I II II II II II I g982 GSGEYGDMIGMGVLDPAKVTRSALQHAASIAGLMLTTDCMIAEIPEEKPAVPDMGGMGGM 490 500 510 520 530 540 m982.pep GGMMX Mill g982 GGMMX The following partial DNA sequence was identified in N meningitidis <SEQ ID 2965>: a982.seq 1 ATGGCAGCAA AAGACGTACA ATTCGGCAAT GAAGTCCGCC AAAAAATGGT 51 AAACGGCGTG AACATTTTGG CAAACGCCGT GCGCGTAACC TTGGGTCCCA 101 AAGGCCGCAA CGTGGTGGTT GACCGCGCTT TCGGCGGCCC GCACATCACC 151 AAAGACGGCG TAACCGTCGC CAAAGAAATC GAACTGAAAG ACAAGTTTGA 201 AAATATGGGC GCGCAAATGG TGAAAGAAGT CGCGTCCAAA ACCAACGACG 251 TGGCGGGCGA CGGTACGACT ACCGCCACCG TATTGGCGCA ATCCATCGTT 301 GCCGAAGGTA TGAAATACGT TACCGCCGGT ATGAACCCGA CCGACCTGAA 351 ACGCGGTATC GACAAAGCCG TCGCCGCTTT GGTTGAAGAG CTGAAAAACA 401 TCGCCAAACC TTGCGACACT TCTAAAGAAA TCGCCCAAGT CGGCTCTATT 451 TCCGCCAACT CTGACGAACA AGTCGGCGCG ATTATTGCCG AAGCGATGGA 501 AAAAGTCGGC AAAGAAGGCG TGATTACCGT TGAAGACGGC AAATCTTTGG 551 AAAACGAGCT GGACGTGGTT GAAGGTATGC AATTCGACCG CGGCTACCTG 601 TCTCCTTACT TCATCAACGA TGCGGAAAAA CAAATCGCCG GCTTGGACAA 651 TCCGTTTGTA TTGCTGTTCG ACAAAAAAAT CAGCAATATC CGCGACCTGC 701 TGCCTGTTTT GGAACAAGTG GCCAAAGCCA GCCGTCCGCT GTTGATTATC 751 GCTGAAGACG TAGAAGGCGA AGCCTTGGCG ACTTTGGTCG TGAACAACAT 801 CCGCGGCATT CTGAAAACCG TTGCCGTTAA AGCTCCGGGC TTCGGCGACC 851 GCCGCAAAGC GATGCTGCAA GACATCGCTA TCCTGACCGG CGGCACAGTG 901 ATTTCCGAAG AAGTCGGCCT GTCTTTGGAA AAAGCGACTT TGGACGACTT 951 GGGTCAGGCC AAACGCATCG AAATCGGTAA AGAAAACACC ACCATCATCG 1001 ACGGCTTCGG CGACGCAGCC CAAATCGAAG CGCGTGTTGC CGAAATCCGC 1051 CAACAAATCG AAACCGCAAC CAGCGATTAC GACAAAGAAA AACTGCAAGA 1101 GCGCGTTGCC AAACTGGCAG GCGGCGTGGC AGTAATCAAA GTCGGTGCCG 1151 CGACCGAAGT GGAAATGAAA GAGAAAAAAG ACCGCGTGGA AGACGCGCTG 1201 CACGCTACCC GCGCAGCCGT TGAAGAAGGC GTGGTTGCAG GCGGCGGCGT 1251 AGCCCTGTTG CGCGCCCGTG CCGCTCTGGA AAACCTGCAC ACCGGCAATG 1301 CAGACCAAGA CGCAGGCGTA CAAATCGTCT TGCGCGCCGT TGAGTCTCCG 1351 CTGCGCCAAA TCGTTGCCAA CGCAGGCGGC GAACCCAGCG TGGTTGTGAA 1401 CAAAGTGTTG GAAGGCAAAG GCAACTATGG TTACAACGCT GGCAGCGGCG 1451 AATACGGCGA CATGATCGAA ATGGGCGTAC TCGACCCCGC CAAAGTAACC 1501 CGTTCCGCGC TGCAACACGC CGCGTCTATC GCCGGCCTGA TGCTGACCAC 1551 AGACTGCATG ATTGCTGAAA TCCCTGAAGA CAAACCGGCT ATGCCTGATA 1601 TGGGCGGCAT GGGTGGTATG GGCGGCATGA TGTAA This corresponds to the amino acid sequence <SEQ ID 2966; ORF 982. a>: a982.pep 1 MAAKDVQFGN EVRQKMVNGV NILANAVRVT LGPKGRNWV DRAFGGPHIT 51 KDGVTVAKEI ELKDKFENMG AQMVKEVASK TNDVAGDGTT TATVLAQSIV 101 AEGMKYVTAG MNPTDLKRGI DKAVAALVEE LKNIAKPCDT SKEIAQVGSI 151 SANSDEQVGA IIAEAMEKVG KEGVITVEDG KSLENELDVV EGMQFDRGYL 201 SPYFINDAEK QIAGLDNPFV LLFDKKISNI RDLLPVLEQV AKASRPLLII 251 AEDVEGEALA TLWNNIRGI LKTVAVKAPG FGDRRKAMLQ DIAILTGGTV 301 ISEEVGLSLE KATLDDLGQA KRIEIGKENT TIIDGFGDAA QIEARVAEIR 351 QQIETATSDY DKEKLQERVA KLAGGVAVIK VGAATEVEMK EKKDRVEDAL 401 HATRAAVEEG VVAGGGVALL RARAALENLH TGNADQDAGV QIVLRAVESP 451 LRQIVANAGG EPSVWNKVL EGKGNYGYNA GSGEYGDMIE MGVLDPAKVT 501 RSALQHAASI AGLMLTTDCM IAEIPEDKPA MPDMGGMGGM GGMM* m982/a982 99.3% identity in 544 aa overlap 10 20 30 40 50 60 m982. pep MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVWDRAFGGPHITKDGVTVAKEI II II II II II II I II I II I II II II I II I II II II II II II II I II II I II II II II II I a982 MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVWDRAFGGPHITKDGVTVAKEI 10 20 30 40 50 60 70 80 90 100 110 120 m982. pep ELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAEGMKYVTAGMNPTDLKRGI II II I I I II I II II II II I II II II II I I II II II II I II II II II II I II II II II I II a982 ELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAEGMKYVTAGMNPTDLKRGI 70 80 90 100 110 120 130 140 150 160 170 180 m982. pep DKAVAALVDELKNIAKPCDTSKEIAQVGSISANSDEQVGAIIAEAMEKVGKEGVITVEDG I II II II M II II II I II I II II I I I II I II I II I I II II II II II I II II II I I II I II a982 DKAVAALVEELKNIAKPCDTSKEIAQVGSISANSDEQVGAIIAEAMEKVGKEGVITVEDG 130 140 150 160 170 180 190 200 210 220 230 240 m982. pep KSLENELDVVEGMQFDRGYLSPYFINDAEKQIAALDNPFVLLFDKKISNIRDLLPVLEQV II I II II II II II II I II II II II II II I II I M II II II II I I I I I II II II II I I I II a982 KSLENELDVVEGMQFDRGYLSPYFINDAEKQIAGLDNPFVLLFDKKISNIRDLLPVLEQV 190 200 210 220 230 240 250 260 270 280 290 300 m982 . pep AKASRPLLI IAEDVEGEALATLVVNNIRGILKTVAVKAPGFGDRRKAMLQDIAILTGG I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I a 982 AKASRPLLIIAEDVEGEALATLWNNIRGILKTVAVKAPGFGDRRKAMLQDIAILTGGTV 250 260 270 280 290 300 310 320 330 340 350 360 m982 . pep ISEEVGLSLEKATLDDLGQAKRIEIGKENTTIIDGFGDAAQIEARVAEIRQQIETATSDY I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I a982 ISEEVGLSLEKATLDDLGQAKRIEIGKENTTIIDGFGDAAQIEARVAEIRQQIETATSDY 310 320 330 340 350 360 370 380 390 400 410 420 m982. pep DKEKLQERVAKLAGGVAVIKVGAATEVEMKEKKDRVEDALHATRAAVEEGVVAGGGVALL II II I II II II II I II II I II II I I II I II II II II II II II II II I II II II II II II I a982 DKEKLQERVAKLAGGVAVIKVGAATEVEMKEKKDRVEDALHATRAAVEEGWAGGGVALL 370 380 390 400 410 420 430 440 450 460 470 480 m982. pep RARAALENLHTGNADQDAGVQIVLRAVESPLRQIVANAGGEPSVWNKVLEGKGNYGYNA MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a982 RARAALENLHTGNADQDAGVQIVLRAVESPLRQIVANAGGEPSVWNKVLEGKGNYGYNA 430 440 450 460 470 480 490 500 510 520 530 540 m982. pep GSGEYGDMIEMGVLDPAKVTRSALQHAASIAGLMLTTDCMIAEIPEDKPAVPDMGGMGGM I I II I I II II II I II I II II II II II I II II I I II II II II II I I II I I I : II I II I II I a982 GSGEYGDMIEMGVLDPAKVTRSALQHAASIAGLMLTTDCMIAEIPEDKPAMPDMGGMGGM 490 500 510 520 530 540 m982.pep GGMMX I II II a982 GGMMX The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2967>: g986. seq 1 GTGTTCAAAA AATACCAATA CTTCGCTTTG GCGGCACTGT GTGCCGCCTT 51 GCTGGCAGGC TGCGAAAAGG CAGGCAGCTT TTTCGGTGCG GACAAAAAAG 101 AAGCATCCTT CGTAGAACGC ATCGAACACA CCAAAGACGA CGGCAGTGTC 151 AGTATGCTGC TGCCCGACTT TGCCCAACTG GTTCAAAGCG AAGGCCCGGC 201 AGTCGTCAAT ATTCAGGCAG CCCCCGCCCC GCGCACCCAA AACGGCAGCG 251 GCAATGCCGA AACCGATTCC GACCCGCTTG CCGACAGCGA CCCGTTCTAC 301 GAATTTTTCA AACGCCTCGT CCCGAACATG CCCGAAATCC CCCAAGAAGA 351 AGCAGATGAC GGCGGATTGA ACTTCGGTTC GGGCTTCATC ATCAGCAAAA 401 ACGGCTACAT CCTGACCAAT ACCCACGTCG TTGCCGGTAT GGGCAGTATC 451 AAAGTCCTGC TCAACGACAA GCGCGAATAT ACCGCCAAAC TCATCGGTTC 501 GGATGTCCAA TCCGATGTCG CCCTTCTGAA AATCGACGCA ACGGAAGAGC 551 TACCCGTCGT CAAAATCGGC AATCCCAAAA ATTTGAAACC GGGCGAATGG 601 GTCGCTGCCA TCGGCGCGCC CTTCGGCTTT GACAACAGCG TGACCGCCGG 651 CATCGTGTCC GCCAAAGGCA GAAGCCTGCC CAACGAAAgc tACACACCCT 701 TCATCCAAAC CGACGTTGCC ATCAATCCGG GCAATTCCGG CGGCCCGCTG 751 TTCAACTTAA AAGGACAGGt cgTCGGCATC AATTCGCAAA TATACAGCCG 801 CAGCGgcgga ttCATGGGCA TCTCCTTTGC CATCCCGATT GACGTTGCCA 851 TGAATGTCGC CGAACAGCTG AAAAACACCG GCAAAGTCCA ACGCGGACAA 901 CTGGGCGTGA TTATTCAGGA AGTATCCTAC GGTTTGGCAC AGTCGTTCGG 951 TCTGGATAAA GCCAGCGGCG CATTGATTGC CAAAATCCTT CCCGGCAGCC 1001 CCGCAGAACG TGCCGGCCTG CAGGCGGGCG ACATCGTCCT CAGCCTCGAC 1051 GGCGGAGAAA TACGTTCTTC CGGCGACCTT CCCGTCATGG TCGGCGCCAT 1101 TACGCCGGGA AAAGAAGTCA GCCTCGGCGT ATGGCGCAAA GGCGAAGAAA 1151 TCACAATCAA AGCCAAGCTG GGCAACGCCg ccgagcATAC CGGCgcatCA 1201 TCCAAAACAG ATGAAgcccc ctacaccgAA CAGCAATCCG GTACGTTCTC 1251 GGTCGAATCC GCAGGCATTA CCCTTCAGAC ACATACCGAC AGCAGCGGca 1301 aacacctcgt cgtcgtacgg gtttccgacg cggcagaacg cGCAGGCTTA 1351 AGgcgcggcg acgaaatcct cgcggtcggg caagtccccg tcaatgacga 1401 agccgGTTTC cgcaaaGCTA TGGACAAGGC AGGCAAAAAC GTCCCCCTGC 1451 TGGTCAtgcg ccgTGGCAAC ACGCTGTTCA TCGCATTAAA CCTGCAATAA This corresponds to the amino acid sequence <SEQ ID 2968; ORF 986.ng>: g986.pep 1 VFKKYQYFAL AALCAALLAG CEKAGSFFGA DKKEASFVER IEHTKDDGSV 51 SMLLPDFAQL VQSEGPAWN IQAAPAPRTQ NGSGNAETDS DPLADSDPFY 101 EFFKRLVPNM PEIPQEEADD GGLNFGSGFI ISKNGYILTN THWAGMGSI 151 KVLLNDKREY TAKLIGSDVQ SDVALLKIDA TEELPVVKIG NPKNLKPGEW 201 VAAIGAPFGF DNSVTAGIVS AKGRSLPNES YTPFIQTDVA INPGNSGGPL 251 FNLKGQWGI NSQIYSRSGG FMGISFAIPI DVAMNVAEQL KNTGKVQRGQ 301 LGVIIQEVSY GLAQSFGLDK ASGALIAKIL PGSPAERAGL QAGDIVLSLD 351 GGEIRSSGDL PVMVGAITPG KEVSLGVWRK GEEITIKAKL GNAAEHTGAS 401 SKTDEAPYTE QQSGTFSVES AGITLQTHTD SSGKHLWVR VSDAAERAGL 451 RRGDEILAVG QVPVNDEAGF RKAMDKAGKN VPLLVMRRGN TLFIALNLQ* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2969>: m986. seq 1 GTGTTCAAAA AATACCAATA CCTCGCTTTG GCAGCACTGT GTGCAGCCTC 51 GCTGGCAGGC TGCGACAAGG CAGGCAGCTT CTTCGTGGCG GACAAAAAAG 101 AAGCATCCTT CGTAGAACGC ATCGAACACA CCAAAGACGA CGGCAGCGTC 151 AGTATGCTGC TGCCCGACTT TGCCCAACTG GTTCAAAGTG AAGGTCCGGC 201 AGTCGTCAAT ATTCAGGCAG CCCCCGCCCC GCGCACCCAA AACGGCAGCG 251 GCAATGCCGA AAACGATTCC GACCCGATTG CCGACAACGA CCCGTTCTAC 301 GAATTTTTCA AACGCCTCGT CCCGAATATG CCCGAAATCC CCCAAGAAGA 351 AGCAGATGAC GGCGGATTGA ACTTCGGTTC GGGCTTCATC ATCAGCAAAG 401 ACGGCTACAT CCTGACCAAT ACCCACGTCG TTACCGGCAT GGGCAGTATC 451 AAAGTCCTGC TCAACGACAA GCGCGAATAT ACCGCCAAAC TCATCGGTTC 501 GGATGTCCAA TCCGATGTCG CCCTTCTGAA AATCGACGCA ACGGAAGAGC 551 TGCCCGTCGT CAAAATCGGC AATCCCAAAG ATTTGAAACC GGGCGAATGG 601 GTCGCCGCCA TCGGCGCGCC CTTCGGCTTC GACAACAGCG TGACCGCCGG 651 CATCGTGTCC GCCAAAGGCA GAAGCCTGCC CAACGAAAGC TACACACCCT 701 TCATCCAAAC CGACGTTGCC ATCAATCCGG GCAACTCCGG CGGCCCGCTG 751 TTCAACTTAA AAGGACAGGT CGTCGGCATC AACTCGCAAA TATACAGCCG 801 CAGCGGCGGA TTCATGGGCA TTTCCTTCGC CATCCCGATT GACGTTGCCA 851 TGAATGTCGC CGAACAGCTG AAAAACACCG GCAAAGTCCA ACGCGGACAA 901 CTGGGCGTGA TTATTCAAGA AGTATCCTAC GGTTTGGCAC AATCGTTCGG 951 TTTGGACAAA GCCGGCGGCG CACTGATTGC CAAAATCCTG CCCGGCAGCC 1001 CCGCAGAACG TGCCGGCCTG CAGGCGGGCG ACATCGTCCT CAGCCTCGAC 1051 GGCGGAGAAA TACGTTCTTC CGGCGACCTT CCCGTTATGG TCGGCGCCAT 1101 TACGCCGGGA AAAGAAGTCA GCCTCGGCGT ATGGCGCAAA GGCGAAGAAA 1151 TCACAATCAA AGTCAAGCTG GGCAACGCCG CCGAGCATAT CGGCGCATCA 1201 TCCAAAACAG ATGAAGCCCC CTACACCGAA CAGCAATCCG GTACGTTCTC 1251 GGTCGAATCC GCAGGCATTA CCCTTCAGAC ACATACCGAC AGCAGCGGCG 1301 GACACCTCGT CGTCGTACGG GTTTCCGACG CGGCAGAACG CGCAGGCTTG 1351 AGGCGCGGCG ACGAAATTCT TGCCGTCGGG CAAGTCCCCG TCAATGACGA 1401 AGCCGGTTTC CGCAAAGCTA TGGACAAGGC AGGCAAAAAC GTCCCCCTGC 1451 TGATCATGCG CCGTGGCAAC ACGCTGTTTA TCGCATTAAA CCTGCAATAA This corresponds to the amino acid sequence <SEQ ID 2970; ORF 986>: m986.pep.. 1 VFKKYQYLAL AALCAASLAG CDKAGSFFVA DKKEASFVER IEHTKDDGSV 51 SMLLPDFAQL VQSEGPAWN IQAAPAPRTQ NGSGNAENDS DPIADNDPFY 101 EFFKRLVPNM PEIPQEEADD GGLNFGSGFI ISKDGYILTN THWTGMGSI 151 KVLLNDKREY TAKLIGSDVQ SDVALLKIDA TEELPVVKIG NPKDLKPGEW 201 VAAIGAPFGF DNSVTAGIVS AKGRSLPNES YTPFIQTDVA INPGNSGGPL 251 FNLKGQWGI NSQIYSRSGG FMGISFAIPI DVAMNVAEQL KNTGKVQRGQ 301 LGVIIQEVSY GLAQSFGLDK AGGALIAKIL PGSPAERAGL QAGDIVLSLD 351 GGEIRSSGDL PVMVGAITPG KEVSLGVWRK GEEITIKVKL GNAAEHIGAS 401 SKTDEAPYTE QQSGTFSVES AGITLQTHTD SSGGHLVWR VSDAAERAGL 451 RRGDEILAVG QVPVNDEAGF RKAMDKAGKN VPLLIMRRGN TLFIALNLQ* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m986/g986 97.0% identity in 499 aa overlap 10 20 30 40 50 60 m986. pep VFKKYQYLALAALCAASLAGCDKAGSFFVADKKEASFVERIEHTKDDGSVSMLLPDFAQL II I I I I I : II II II II II II : I II II I I I II II II II II II II I I I II I II II I I II I g986 VFKKYQYFALAALCAALLAGCEKAGSFFGADKKEASFVERIEHTKDDGSVSMLLPDFAQL 10 20 30 40 50 60 70 80 90 100 110 120 m986.pep VQSEGPAWΝIQAAPAPRTQΝGSGΝAEΝDSDPIADΝDPFYEFFKRLVPΝMPEIPQEEADD I II II II II II I II I I II II II I I II I : II II : I I : II II I I II I I I I I I II II I II II I g986 VQSEGPAWΝIQAAPAPRTQΝGSGΝAETDSDPLADSDPFYEFFKRLVPΝMPEIPQEEADD 70 80 90 100 110 120 130 140 150 160 170 180 m986.pep GGLΝFGSGFIISKDGYILTΝTHVVTGMGSIKVLLΝDKREYTAKLIGSDVQSDVALLKIDA II I II II II II I I : I II II II I I I : II I I I II II II II I II II I II I I II I II I I I I I I I g986 GGLNFGSGFIISKΝGYILTΝTHWAGMGSIKVLLΝDKREYTAKLIGSDVQSDVALLKIDA 130 140 150 160 170 180 190 200 210 220 230 240 m986.pep TEELPWKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGIVSAKGRSLPNESYTPFIQTDVA MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g986 TEELPVVKIGNPKNLKPGEWVAAIGAPFGFDNSVTAGIVΞAKGRSLPNESYTPFIQTDVA 190 200 210 220 230 240 250 260 270 280 290 300 m986.pep INPGNSGGPLFNLKGQWGINSQIYSRSGGFMGISFAIPIDVAMNVAEQLKNTGKVQRGQ I II II II II II II II II I II II II II II II I II II I I II II I II II I I II I I II II I II I g986 INPGNSGGPLFNLKGQWGINSQIYSRSGGFMGISFAIPIDVAMNVAEQLKNTGKVQRGQ 250 260 270 280 290 300 310 320 330 340 350 360 m986.pep LGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSPAERAGLQAGDIVLSLDGGEIRSSGDL II I II II II II II II II II I I : II II I I I II II II II II II I I I I I I I I II II II II II I g986 LGVIIQEVSYGLAQSFGLDKASGALIAKILPGSPAERAGLQAGDIVLSLDGGEIRSSGDL 310 320 330 340 350 360 370 380 390 400 410 420 m986.pep PVMVGAITPGKEVSLGVWRKGEEITIKVKLGNAAEHIGASSKTDEAPYTEQQSGTFSVES II I II II II I I II II I I I I II II II II : II II II II II II II II II I I I II I II II I II g986 PVMVGAITPGKEVSLGVWRKGEEITIKAKLGNAAEHTGASSKTDEAPYTEQQSGTFSVES 370 380 390 400 410 420 430 440 450 460 470 480 m986. pep AGITLQTHTDSSGGHLWVRVSDAAERAGLRRGDEILAVGQVPVNDEAGFRKAMDKAGKN lllllllllllll I I II II II II II II I II II II II I II II II II I I II II II I II I I I g986 AGITLQTHTDSSGKHLVWRVSDAAERAGLRRGDEILAVGQVPVNDEAGFRKAMDKAGKN 430 440 450 460 470 480 490 500 m986.pep VPLLIMRRGNTLFIALNLQX II I I : II II II II II II I II g986 VPLLVMRRGNTLFIALNLQX 490 500 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2971>: a986. seq 1 GTGTTCAAAA AATACCAATA CCTCGCTTTG GCAGCACTGT GTGCCGCCTC 51 GCTGGCAGGC TGCGACAAAG CCGGCAGCTT TTTCGGTGCG GACAAAAAAG 101 AAGCATCCTT TGTAGAACGC ATCAAACACA CCAAAGACGA CGGCAGCGTC 151 AGTATGCTGC TGCCCGACTT TGTCCAACTG GTTCAAAGCG AAGGCCCGGC 201 AGTCGTCAAT ATTCAGGCAG CCCCCGCCCC GCGCACCCAA AACGGCAGCA 251 GCAATGCCGA AACCGATTCC GACCCGCTTG CCGACAGCGA CCCGTTCTAC 301 GAATTTTTCA AACGCCTCGT CCCGAACATG CCCGAAATCC CCCAAGAAGA 351 AGCAGATGAC GGΝGGATTGA ACTTCGGTTC GGGCTTCATC ATCAGCAAAG 401 ACGGCTATAT TCTGACCAAT ACGCACGTCG TTACCGGCAT GGGCAGTATC 451 AAAGTCCTGC TCAACGACAA GCGCGAATAT ACCGCCAAAC TCATCGGTTC 501 GGATGTCCAA TCCGATGTCG CCCTTCTGAA AATCGACGCA ACGGAAGAGC 551 TGCCCGTCGT CAAAATCGGC AATCCCAAAG ATTTGAAACC GGGCGAATGG 601 GTCGCCGCCA TCGGCGCGCC CTTCGGCTTC GACAACAGCG TGACCGCCGG 651 CΝTCGTGTCC GCCAAAGGCA GAAGCCTGCC CAACGAAAGC TACACACCCT 701 TCATCCAAAC CGACGTTGCC ATCAATCCGG GCAACTCCGG CGGCCCGCTG 751 TTCAACTTAA AAGGACAGGT CGTCGGCATC AACTCGCAAA TATACAGCCG 801 CAGCGGCGGA TTCATGGGCA TTTCCTTCGC CATCCCGATT GACGTTGCCA 851 TGAATGTCGC CGAACAGCTG AAAAACACCG GCAAAGTCCA ACGCGGACAA 901 CTGGGCGTGA TTATTCAAGA AGTATCCTAC GGTTTGGCAC AATCGTTCGG 951 TTTGGACAAA GCCGGCGGCG CACTGATTGC CAAAATCCTG CCCGGCAGCC 1001 CCGCAGAACG TGCCGGCCTG CGGGCGGGCG ACATCGTCCT CAGCCTCGAC 1051 GGCGGAGAAA TACGTTCTTC CGGCGACCTT CCCGTTATGG TCGGCGCCAT 1101 TACGCCGGGA AAAGAAGTCA GCCTCGGCGT ATGGCGCAAA GGCGAAGAAA 1151 TCACAATCAA AGTCAAGCTG GGCAACGCCG CCGAGCATAT CGGCGCATCA 1201 TCCAAAACAG ATGAAGCCCC CTACACCGAA CAGCAATCCG GTACGTTCTC 1251 GGTCGAATCC GCAGGCATTA CCCTTCAGAC ACATACCGAC AGCAGCGGCG 1301 GACACCTCGT CGTCGTACGG GTTTCCGACG CGGCAGAACG CGCAGGCTTG 1351 AGGCGCGGCG ACGAAATTCT TGCCGTCGGG CAAGTCCCCG TCAATGACGA 1401 AGCCGGTTTC CGCAAAGCTA TGGACAAGGC AGGCAAAAAC GTCCCCCTGC 1451 TGATCATGCG CCGTGGCAAC ACGCTGTTTA TCGCATTAAA CCTGCAATAA This corresponds to the amino acid sequence <SEQ ID 2972; ORF 986. a>: a986.pep 1 VFKKYQYLAL AALCAASLAG CDKAGSFFGA DKKEASFVER IKHTKDDGSV 51 SMLLPDFVQL VQSEGPAWN IQAAPAPRTQ NGSSNAETDS DPLADSDPFY 101 EFFKRLVPNM PEIPQEEADD GGLNFGSGFI ISKDGYILTN THWTGMGSI 151 KVLLNDKREY TAKLIGSDVQ SDVALLKIDA TEELPVVKIG NPKDLKPGEW 201 VAAIGAPFGF DNSVTAGXVS AKGRSLPNES YTPFIQTDVA INPGNSGGPL 251 FNLKGQWGI NSQIYSRSGG FMGISFAIPI DVAMNVAEQL KNTGKVQRGQ 301 LGVIIQEVSY GLAQSFGLDK AGGALIAKIL PGSPAERAGL RAGDIVLSLD 351 GGEIRSSGDL PVMVGAITPG KEVSLGVWRK GEEITIKVKL GNAAEHIGAS 401 SKTDEAPYTE QQSGTFSVES AGITLQTHTD SSGGHLVWR VSDAAERAGL 451 RRGDEILAVG QVPVNDEAGF RKAMDKAGKN VPLLIMRRGN TLFIALNLQ* m986/a986 ,2% identity in 499 aa overlap 10 20 30 40 50 60 m986.pep VFKKYQYLALAALCAASLAGCDKAGSFFVADKKEASFVERIEHTKDDGSVSMLLPDFAQL II II I II II I II I II II II II I I II I II II I II II I I I I I : II II I II I II II II I : II a986 VFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIKHTKDDGSVSMLLPDFVQL 10 20 30 40 50 60 70 80 90 100 110 120 m986.pep VQSEGPAVVNIQAAPAPRTQNGSGNAENDSDPIADNDPFYEFFKRLVPNMPEIPQEEADD II II I II II II II II II I II I I I : II I: II II : I I : II I II II I I I II II II I II II II I a986 VQSEGPAWNIQAAPAPRTQNGSSNAETDSDPLADSDPFYEFFKRLVPNMPEIPQEEADD 70 80 90 100 110 120 130 140 150 160 170 180 m986.pep GGLNFGSGFIISKDGYILTNTHWTGMGSIKVLLNDKREYTAKLIGSDVQSDVALLKIDA I II II II II I II II II II II I II I II II I II II I I I I II I I I II II II II I I I I I II I I I a986 GGLNFGSGFI ISKDGYILTNTHWTGMGSIKVLLNDKREYTAKLIGSDVQSDVALLKIDA 130 140 150 160 170 180 190 200 210 220 230 240 m986.pep TEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGIVSAKGRSLPNESYTPFIQTDVA II II I II II II II I II II II II II II II I I II II II I II II II II I II II I II II II II a986 TEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGXVSAKGRSLPNESYTPFIQTDVA 190 200 210 220 230 240 250 260 270 280 290 300 m986.pep INPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISFAIPIDVAMNVAEQLKNTGKVQRGQ I II II I II I I II I II I II II II II II I I II II I II I II I II I II I II II II I I I II II II a986 INPGNSGGPLFNLKGQWGINSQIYSRSGGFMGISFAIPIDVAMNVAEQLKNTGKVQRGQ 250 260 270 280 290 300 310 320 330 340 350 360 m986.pep LGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSPAERAGLQAGDIVLSLDGGEIRSSGDL II I II II II II II II I II I II II II II II II II II II II I : I I I I II I II I I I I I II II I a986 LGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSPAERAGLRAGDIVLSLDGGEIRSSGDL 310 320 330 340 350 360 370 380 390 400 410 420 m986.pep PVMVGAITPGKEVSLGVWRKGEEITIKVKLGNAAEHIGASSKTDEAPYTEQQSGTFSVES I I II II II II II I II I II II II I I II I II II I II II II I II I II I II I II II II I II II I a986 PVMVGAITPGKEVSLGVWRKGEEITIKVKLGNAAEHIGASSKTDEAPYTEQQSGTFSVES 370 380 390 400 410 420 430 440 450 460 470 480 m986. pep AGITLQTHTDSSGGHLVWRVSDAAERAGLRRGDEILAVGQVPVNDEAGFRKAMDKAGKN II II II II I I II II I I II II II II II I I II II II II II I I II II I II II I II II I II I I I a986 AGITLQTHTDSSGGHLVWRVSDAAERAGLRRGDEILAVGQVPVNDEAGFRKAMDKAGKN 430 440 450 460 470 480 490 500 m986.pep VPLLIMRRGNTLFIALNLQX I II II II II II II II I II II a986 VPLLIMRRGNTLFIALNLQX 490 500 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2973>: g987.seq 1 ATGAAAACAC GCAGCCTCAT TTCCCTTTTA TGCCTCCTTC TCTGTTCATG 51 TTCTTCATGG TTGCCCCCAC TGGAAGAACG GACGGAAAGC CGTCATTTTA 101 ATACTTCCAA ACCTGTCCTC CTGGACAACA TCCTGCAAAT CCGGCACACC 151 CCTCATAACA ACGGGCTATC CGACATCTAC CTGCTCGACG ACCCCCACGA 201 AGCCTTTGCC GCCCGCGCCG CCCTTATCGA ATCTGCCGAA CACAGCCTCG 251 ATTTGCAATA CTACATTTGG CGCAACGaCA TTTCCGGCAG GCTGCTGTTC 301 AACCTCATGT ACCTTGCCGC agaacgcGGC GTGCGCGTAC GCCTGCTGTt 351 ggacgacaAC AACAcgcgcg gcttggacga tctcctGCTC GCCCTCGACA 401 GCCATCCCAA TAtctaagtG CGCCTGTTCA ACCCCTtcgt CCTACGCAAA 451 TGGCGCGCAC TCGGCTACCT GACCGACTTC CCCCGCCTCA ACCGCCGCAT 501 GCACAACAAA TCCTTTACCG CCGACAACCG CGCCACCATA CTCGGCGGAC 551 GCAATATCGG CGACGAATAC TTCAAAGTCG GTGAGGACAC CGTTTTCGCC 601 GACCTGGACA TCCTCGCCAC CGGCAGCGTC GTCGGCGAAG TATCGCACGA 651 CTTCGACCGC TACTGGGCAA GCCATTCCGC CCACAACGCC ACGCGCATCA 701 TCCGCAGCGG CAACATCGGC AAGGGTCTTC AAGCACTCGG ATACAACGAC 751 GAAACATCCA GACACGCGCT CCTGCGCTAC CGCGAAACCG TCGAACAGTC 801 GCCCCTCTAC CAAAAAATAC AGACGGGACG CATCGACTGG CAGAGCGTCC 851 AAACCCGCCT GATCAGCGAC AGCCCTGCAA AAGGACTCGA CCGCGACCGC 901 CGCAAACCGC CGATTGCCGG GAGGCTGCAA GACGCGCTCA AACAGCCCGA 951 AAAAAGCGTC TATCTGGTTT CACCCTATTT CGTCCCTACA AAATCCGGCA 1001 CAGACGCACT GGCAAAACTG GTGCAGGACG GCATAGACGT TACCGTCCTG 1051 ACCAACTCGC TACAGGCGAC CGACGTTGCC GCCGTCCATT CCGGCTACGT 1101 CAAATACCGA AAACCGCTGC TCAAAGCCGG CATCAAACTC TACGAGCTGC 1151 AACCCAACCA TGCCGTCCCC GCCACAAAAG ACAAAGGCCT GACCGGCAGC 1201 TCCGTAACCA GCCTGCATGC CAAAACCTTC ATTGTGGacg gCAAACGCAT 1251 CTTCATCGGC TCATTCAACC TCGACCCCCG TTCCGCACGG CTCAATACCG 1301 AAATGGGCGT CGTCATCGAA AGCCCCAAAA TCGCAGAACA GATGGAGCGC 1351 AccctCGCCG AtacCACACC CGAATACGCC TACCGCGTTA CCCTCGACAA 1401 ACACAACCGC CTGCAATGGC ACGATCCCGC CACCCGAAAA ACCTACCCGA 1451 ACGAACCCGA AGCCAAACTT TGGAAACGCA TCGCCGCAAA AATCCTATCC 1501 CTGCTGCCCA TCGAAGGTTT ATTATAG This corresponds to the amino acid sequence <SEQ ID 2974; ORF 987.ng>: g987, pep 1 MKTRSLISLL CLLLCSCSSW LPPLEERTES RHFNTSKPVL LDNILQIRHT 51 PHNNGLSDIY LLDDPHEAFA ARAALIESAE HSLDLQYYIW RNDISGRLLF 101 NLMYLAAERG VRVRLLLDDN NTRGLDDLLL ALDSHPNI*V RLFNPFVLRK 151 WRALGYLTDF PRLNRRMHNK SFTADNRATI LGGRNIGDEY FKVGEDTVFA 201 DLDILATGSV VGEVSHDFDR YWASHSAHNA TRIIRSGNIG KGLQALGYND 251 ETSRHALLRY RETVEQSPLY QKIQTGRIDW QSVQTRLISD SPAKGLDRDR 301 RKPPIAGRLQ DALKQPEKSV YLVSPYFVPT KSGTDALAKL VQDGIDVTVL 351 TNSLQATDVA AVHSGYVKYR KPLLKAGIKL YELQPNHAVP ATKDKGLTGS 401 SVTSLHAKTF IVDGKRIFIG SFNLDPRSAR LNTEMGVVIE SPKIAEQMER 451 TLADTTPEYA YRVTLDKHNR LQWHDPATRK TYPNEPEAKL WKRIAAKILS 501 LLPIEGLL* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2975>: m987. seq 1 ATGAAAACAC GCAGCCTAAT TTCCCTTTTA TGCCTCCTTC TCTGTTCATG 51 TTCTTCATGG TTGCCCCCAC TGGAAGAACG GACGGAAAGC CGTCATTTCA 101 ATACTTCCAA ACCCGTCCGC CTGGACAACA TCCTGCAAAT CCGGCACACC 151 CCTCATACCA ACGGGCTATC CGATATCTAT CTGTTGAACG ACCCCCACGA 201 AGCCTTTGCC GCCCGCGCCG CCCTTATCGA ATCTGCCGAA CACAGCCTCG 251 ATTTGCAATA CTACATCTGG CGCAACGACA TTTCCGGCAG GCTGCTGTTC 301 AACCTCGTGT ACCTTGCCGC AGAACGCGGT GTGCGCGTAC GCCTGCTGTT 351 GGACGACAAC AACACGCGCG GATTGGACGA CCTCCTGCTT GCCCTCGACA 401 GCCATCCCAA TATCGAAGTG CGCCTGTTCA ACCCCTTCGT CTTACGAAAA 451 TGGCGCGCAC TCGGCTACCT GACCGACTTC CCCCGCCTCA ACCGCCGCAT 501 GCACAACAAA TCCTTTACCG CCGACAACCG CGCCACCATA CTCGGCGGAC 551 GCAATATCGG CGACGAATAC TTCAAAGTCG GTGAGGACAC CGTTTTCGCC 601 GATTTGGACA TCCTCGCCAC CGGCAGCGTC GTCGGCGAAG TATCGCACGA 651 CTTCGACCGC TACTGGGCAA GCCATTCCGC CCACAACGCC ACGCGCATCA 701 TCCGCAGCGG CGACATCGGC AAGGGTCTTC AAGCACTCGG ATACAACGAC 751 GAAACGTCCA GACACGCGCT CCTGCGCTAC CGCGAAACCG TCGAACAGTC 801 GCCCCTCTAC CAAAAAATAC AGACAGGATG CATCGACTGG CAGAGCGTCC 851 GAACCCGCCT CATCAGCGAC GACCCTGCAA AAGGACTCGA CCGCGACCGC 901 CGCAAACCGC CGATTGCCGG GCGGCTGCAA GACGCGCTCA AACAGCCCGA 951 AAAAAGCGTC TATCTGGTTT CACCCTATTT CGTTCCCACA AAATCCGGCA 1001 CAGACGCACT GGCAAAACTG GTGCAGGACG GCATAGACGT TACCGTTCTG 1051 ACCAACTCGC TGCAGGCGAC CGACGTTGCC GCCGTCCATT CCGGCTATGT 1101 CAAATACCGA AAACCGCTGC TCAAAGCCGG CATCAAACTC TACGAGCTGC 1151 AACCCAACCA TGCCGTCCCC GCCACAAAAG ACAAAGGCCT GACCGGCAGC 1201 TCCGTAACCA GCCTGCACGC CAAAACCTTC ATTGTGGACG GCAAACGCAT 1251 CTTCATCGGT TCGTTCAACC TCGACCCCCG TTCCGCGCGT CTCAACACCG 1301 AAATGGGCGT TGTTATCGAA AGCCCCAAAA TCGCAGAACA GATGGAGCGC 1351 ACCCTTGCCG ATACCACACC CGCCTACGCC TACCGCGTTA CCCTCGACAG 1401 GCACAACCGC CTGCAATGGC ACGATCCCGC CACCCGAAAA ACCTACCCGA 1451 ACGAACCCGA AGCCAAACTT TGGAAACGCA TCGCCGCAAA AATCCTATCC 1501 CTGCTGCCCA TAGAAGGTTT ATTATAG This corresponds to the amino acid sequence <SEQ ID 2976; ORF 987>: m987.pep 1 MKTRSLISLL CLLLCSCSSW LPPLEERTES RHFNTSKPVR LDNILQIRHT 51 PHTNGLSDIY LLNDPHEAFA ARAALIESAE HSLDLQYYIW RNDISGRLLF 101 NLVYLAAERG VRVRLLLDDN NTRGLDDLLL ALDSHPNIEV RLFNPFVLRK 151 WRALGYLTDF PRLNRRMHNK SFTADNRATI LGGRNIGDEY FKVGEDTVFA 201 DLDILATGSV VGEVSHDFDR YWASHSAHNA TRIIRSGDIG KGLQALGYND 251 ETSRHALLRY RETVEQSPLY QKIQTGCIDW QSVRTRLIΞD DPAKGLDRDR 301 RKPPIAGRLQ DALKQPEKSV YLVSPYFVPT KSGTDALAKL VQDGIDVTVL 351 TNSLQATDVA AVHSGYVKYR KPLLKAGIKL YELQPNHAVP ATKDKGLTGS 401 SVTSLHAKTF IVDGKRIFIG SFNLDPRSAR LNTEMGVVIE SPKIAEQMER 451 TLADTTPAYA YRVTLDRHNR LQWHDPATRK TYPNEPEAKL WKRIAAKILS 501 LLPIEGLL* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m987/g987 97.8% identity in 508 aa overlap 10 20 30 40 50 60 m987.pep MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFΝTSKPVRLDΝILQIRHTPHTΝGLSDIY I I I II II I II II II II II I II II II II II I II I II II II II II I II II II I M I II I II g987 MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFΝTSKPVLLDΝILQIRHTPHΝΝGLSDIY 10 20 30 40 50 60 70 80 90 100 110 120 m987.pep LLΝDPHEAFAARAALIESAEHSLDLQYYIWRΝDISGRLLFΝLVYLAAERGVRVRLLLDDΝ II : II I II II II II I II I I II II II I I II I II II II I I I I II : I I II I I I I II II I I II I g987 LLDDPHEAFAARAALIESAEHSLDLQYYIWRΝDISGRLLFΝLMYLAAERGVRVRLLLDDΝ 70 80 90 100 110 120 130 140 150 160 170 180 m987.pep ΝTRGLDDLLLALDSHPΝIEVRLFΝPFVLRKWRALGYLTDFPRLΝRRMHΝKSFTADΝRATI I II II I II II II I I I II I II II II I I II I II II II II II I I I I I II I II II II II I II I g987 ΝTRGLDDLLLALDSHPΝIXVRLFΝPFVLRKWRALGYLTDFPRLΝRRMHΝKSFTADΝRATI 130 140 150 160 170 180 190 200 210 220 230 240 m987. pep LGGRΝIGDEYFKVGEDTVFADLDILATGSWGEVSHDFDRYWASHSAHΝATRIIRSGDIG I I I II I II II II I I II II II II I II II II I II II II II II II II I II I II II II II I : I I g987 LGGRΝIGDEYFKVGEDTVFADLDILATGSWGEVSHDFDRYWASHSAHΝATRIIRSGΝIG 190 200 210 220 230 240 250 260 270 280 290 300 m987. pep KGLQALGYΝDETSRHALLRYRETVEQSPLYQKIQTGCIDWQSVRTRLISDDPAKGLDRDR I II II I II II II II II II I II II II II II I I I II II II I II M I II II M I II I II I II g987 KGLQALGYΝDETSRHALLRYRETVEQSPLYQKIQTGRIDWQSVQTRLISDSPAKGLDRDR 250 260 270 280 290 300 310 320 330 340 350 360 m987.pep RKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGTDALAKLVQDGIDVTVLTΝSLQATDVA MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g987 RKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGTDALAKLVQDGIDVTVLTNSLQATDVA 310 320 330 340 350 360 370 380 390 400 410 420 m987. pep AVHSGYVKYRKPLLKAGIKLYELQPNHAVPATKDKGLTGSSVTSLHAKTFIVDGKRIFIG II I II II II II II I II II II II II II II I II II II I II II I II II II I II II II I I I I I I g987 AVHSGYVKYRKPLLKAGIKLYELQPNHAVPATKDKGLTGSSVTSLHAKTFIVDGKRIFIG 370 380 390 400 410 420 430 440 450 460 470 480 m987. pep SFNLDPRSARLNTEMGVVIESPKIAEQMERTLADTTPAYAYRVTLDRHNRLQWHDPATRK I I II II II I II I I I I II II I II I I II II II II I II I I II II II I I : I II I I II II I I I I g987 SFNLDPRSARLNTEMGWIESPKIAEQMERTLADTTPEYAYRVTLDKHNRLQWHDPATRK 430 440 450 460 470 480 490 500 509 m987 . pep TYPNEPEAKLWKRIAAKILSLLPIEGLLX M M M M M M M M M M M I M M M g987 TYPNEPEAKLWKRIAAKILSLLPIEGLLX 490 500 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2977>: a987.seq 1 ATGAAAACAC GCAGCCTAAT TTCCCTTTTA TGCCTCCTTC TCTGTTCATG 51 TTCTTCATGG TTGCCCCCAC TGGAAGAACG GACGGAAAGC CGTCATTTCA 101 ATACTTCCAA ACCCGTCCGC CTGGACAACA TCCTGCAAAT CCGGCACACC 151 CCTCATACCA ACGGGCTATC CGATATCTAT CTGTTGAACG ACCCCCACGA 201 AGCCTTTGCC GCCCGCGCCG CCCTTATCGA ATCTGCCGAA CACAGCCTCG 251 ATTTGCAATA CTACATCTGG CGCAACGACA TTTCCGGCCG ACTGCTGTTC 301 AACCTCGTGT ACCTTGCCGC AGAACGCGGT GTGCGCGTAC GCCTGCTGTT 351 GGACGACAAC AACACGCGCG GATTGGACGA CCTCCTGCTC GCCCTCGACA 401 GCCATCCCAA TATCGAAGTG CGCCTGTTCA ACCCCTTCGT CTTACGAAAA 451 TGGCGCGCAC TCGGCTACCT GACCGACTTC CCCCGCCTCA ACCGCCGCAT 501 GCACAACAAA TCCTTTACCG CCGACAACCG CGCCACCATA CTCGGCGGAC 551 GCAATATCGG CGACGAATAC TTCAAAGTCG GTGAGGACAC CGTTTTCGCC 601 GACCTGGACA TCCTCGCCAC CGGCAGCGTC GTCGGCGAAG TATCGCACGA 651 CTTCGACCGC TACTGGGCAA GCCATTCCGC CCACAACGCC ACGCGCATCA 701 TCCGCAGCGG CAACATCGGC AAGGGTCTTC AAGCACTCGG ATACAACGAC 751 GAAACGTCCA GACACGCGCT CCTGCGCTAC CGCGAAACCG TCGAACAGTC 801 GCCCCTCTAC CAAAAAATAC AGACAGGACG CATCGACTGG CAGAGCGTCC 851 AAACCCGCCT CATCAGCGAC GACCCTGCAA AAGGACTCGA CCGCGACCGC 901 CGCAAACCGC CGATTGCCGG GCGGCTGCAA GACGCGCTCA AACAGCCCGA 951 AAAAAGCGTC TATCTGGTTT CACCCTATTT CGTCCCCACA AAATCCGGCA 1001 CAGACGCACT GGCAAAACTG GTGCAGGACG GCATAGACGT TACCGTCCTG 1051 ACCAACTCGC TACAGGCGAC CGACGTTGCC GCCGTCCATT CCGGCTATGT 1101 CAAATACCGA AAACCGCTGC TCAAAGCCGG CATCAAACTC TACGAGCTGC 1151 AACCCAACCA TGCCGTCCCT GCCACAAAAG ACAAAGGCCT GACCGGCAGC 1201 TCCGTAACCA GCCTGCATGC CAAAACCTTC ATTGTGGACG GCAAACGCAT 1251 CTTCATCGGC TCATTCAACC TCGACCCCCG TTCCGCACGG CTCAATACTG 1301 AAATGGGCGT TGTTATCGAA AGCCCCAAAA TCGCAGAACA GATGGAGCGC 1351 ACCCTTGCCG ATACCTCACC CGAATACGCC TACCGCGTTA CCCTCGACAG 1401 GCACAACCGC CTGCAATGGC ACGATCCCGC CACCCGAAAA ACCTACCCGA 1451 ACGAACCCGA AGCCAAACTT TGGAAACGCA TCGCCGCAAA AATCCTATCC 1501 CTGCTGCCCA TAGAAAGTTT ATTATAG This corresponds to the amino acid sequence <SEQ ID 550; ORF 2978. a>: a987.pep 1 MKTRSLISLL CLLLCSCSSW LPPLEERTES RHFNTSKPVR LDNILQIRHT 51 PHTNGLSDIY LLNDPHEAFA ARAALIESAE HSLDLQYYIW RNDISGRLLF 101 NLVYLAAERG VRVRLLLDDN NTRGLDDLLL ALDSHPNIEV RLFNPFVLRK 151 WRALGYLTDF PRLNRRMHNK SFTADNRATI LGGRNIGDEY FKVGEDTVFA 201 DLDILATGSV VGEVSHDFDR YWASHSAHNA TRIIRSGNIG KGLQALGYND 251 ETSRHALLRY RETVEQSPLY QKIQTGRIDW QSVQTRLISD DPAKGLDRDR 301 RKPPIAGRLQ DALKQPEKSV YLVSPYFVPT KSGTDALAKL VQDGIDVTVL 351 TNSLQATDVA AVHSGYVKYR KPLLKAGIKL YELQPNHAVP ATKDKGLTGS 401 SVTSLHAKTF IVDGKRIFIG SFNLDPRSAR LNTEMGVVIE SPKIAEQMER 451 TLADTSPEYA YRVTLDRHNR LQWHDPATRK TYPNEPEAKL WKRIAAKILS 501 LLPIESLL* m987/a987 98.8% identity in 508 aa overlap 10 20 30 40 50 60 m987 . pep MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNILQIRHTPHTNGLSDIY M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M a987 MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNILQIRHTPHTNGLSDIY 10 20 30 40 50 60 70 80 90 100 110 120 m987.pep LLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNLVYLAAERGVRVRLLLDDN II I II I II I I II II II II I II II I I II II II II II II II II II II I I I I I II I I I II II I a987 LLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNLVYLAAERGVRVRLLLDDN 70 80 90 100 110 120 130 140 150 160 170 180 m987.pep NTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDFPRLNRRMHNKSFTADNRATI I II II II II I II II I II I II II II II I II II II I II I II II I I II II I I I I II I II II II a987 NTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDFPRLNRRMHNKSFTADNRATI 130 140 150 160 170 180 190 200 210 220 230 240 m987.pep LGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDFDRYWASHSAHNATRIIRSGDIG I II II I II I II II II I II II I II I I II II II II II II II II I II I II II II II I II I : I I a987 LGGRNIGDEYFKVGEDTVFADLDILATGSWGEVSHDFDRYWASHSAHNATRIIRSGNIG 190 200 210 220 230 240 250 260 270 280 290 300 m987.pep KGLQALGYNDETSRHALLRYRETVEQSPLYQKIQTGCIDWQSVRTRLISDDPAKGLDRDR II II II II II II I I II II II I II I II I II II II II I II II II : II I I I I II II II I II I a987 KGLQALGYNDETSRHALLRYRETVEQSPLYQKIQTGRIDWQSVQTRLISDDPAKGLDRDR 250 260 270 280 290 300 310 320 330 340 350 360 m987. pep RKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGTDALAKLVQDGIDVTVLTNSLQATDVA MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a987 RKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGTDALAKLVQDGIDVTVLTNSLQATDVA 310 320 330 340 350 360 370 380 390 400 410 420 m987.pep AVHSGYVKYRKPLLKAGIKLYELQPNHAVPATKDKGLTGSSVTSLHAKTFIVDGKRIFIG II II II II II II I II I I II I II II II I II II II II I I II I II II I II I II I II II I I I I I a987 AVHSGYVKYRKPLLKAGIKLYELQPNHAVPATKDKGLTGSSVTSLHAKTFIVDGKRIFIG 370 380 390 400 410 420 430 440 450 460 470 480 m987. pep SFNLDPRSARLNTEMGWIESPKIAEQMERTLADTTPAYAYRVTLDRHNRLQWHDPATRK II II II II II II II II II II II II II I II II II II : I II II II II I I II II II I II I II a987 SFNLDPRSARLNTEMGWIESPKIAEQMERTLADTSPEYAYRVTLDRHNRLQWHDPATRK 430 440 450 460 470 480 490 500 509 m987.pep TYPNEPEAKLWKRIAAKILSLLPIEGLLX II I II I II II II II II II II I II I I : I I I a987 TYPNEPEAKLWKRIAAKILSLLPIESLLX 490 500 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2979>: g988.seq 1 ATGAATAAAA ATATTAAATC TTTAAATTTA CGGGAAAAAG ACCCGTTTTT 51 AAGTCGTGAA AAACAGCGTT ATGAACATCC TTTGCCCAGT CGGgaATGGA 101 TAATCGAATT GTTGGAGCGC AAAGGTGTGC CTTCAAAAAT CGAATCGCTT 151 GCACGCGAGC TGTCGATTAC GGAAGacgag tATGTCTTTT TTGAACGCCG 201 TCTGAaggCG atgGCGCGGG AcggtCAGGT TTTAATCAAC CGCCgaggcg 251 CagtTTGCGc gGCggacaag ctgGATTTGG TCAAATGccg Cgtcgaggcg 301 catAAgGAcg gtttcggctt cgcCGTGCCG CTCATGCCGA TGGACGAAGG 351 GGATTTCGTT TTATACGAAC GCCAgatgcg tggTGtcatG CAcggcgaca 401 ccgttACCGT CCGTCCTGCg ggtatggaCC GCAGGGGccg ccgcGAAggg 451 acgtttctGG ATATTGTCGA ACGCGCGCAA AGCAAAGTTG TCGGCCGTTT 501 CTATATGGAT AGGGGCGTGG CGATTTTGGA GCCGGAAGAC AAGCGTCTGA 551 ACCAAAGCAT CGTGTTGGAA CCGGACGGCG TGGCGCGTTT CAAACCCGAA 601 TCCGGTCAGG TTATCGTCGG CAAAATTGAG GTTTATCCCG AGCAAAACCG 651 GCCTGCAGTG GCAAAAATCA TTGAAGTTTT GGGCGATTAT GCCGACAGCG 701 GGATGGAAAt cgAAATTGCC GTGCGCAAGC ATCATTTGCC GCAccgaTTC 751 AGTGAagcgt gtGcCAAATC CGcgaaAAAA ATtcccgacc ATGTACGCAA 801 AAGCGATTTG AAAGGCCGCG TCGATTTGTG CGACCTTCCT TTGGTAACGA 851 TAGACGGCGA AACGGCGCGC GATTTCGACG ACGCGGTGTT TGCCGAAAAA 901 GTCGGACGCA ATTACCGCCT GGTCGTGGCG ATTGCGGATG TCAGCCATTA 951 TGTCCGCCCT GACGATGCGA TTGATGCAGA TGCTCAAGAA CGCAGTACCA 1001 GCGTGTATTT CCCGCGCCGT ATGATTCCGA TGCTGCCGGA AAACCTGTCC 1051 AACGGCATCT GCTCGCTCAA TCCCGATGTC GAGCGTTTGT GTATGGTGTG 1101 CGATATGGTC GTTACCTATG CGGGCAATAT CAAAGAATAC CGCTTCTATC 1151 CCGCCGTGAT GCGCTCTCAT GCCCGCCTGA CCTACAACCA AGTTTGGAAA 1201 TGGCTTTCAG ACGGCATCGG GAATCCGCAC AAAGCCCAAA TCGACACGCT 1251 TTACAAGCTG TTTAAAATTT TGCAGAAAAA ACGTCTGGCG CGCGGGGCGG 1301 TGGAGTTTGA AAGCGTCGAA ACCCAGATGA TTTTCGACGA CAACGGCAAA 1351 ATCGAAAAAA TTGTCCCCGT CGTCCGCAAC gatGCCCACA AGCTGATTGA 1401 AGAATGTATG CTGGCGGCGA ATGTTTGCGC GGCGGATTTT CTGTTGAAAA 1451 ACAAACATAC GGCTTTGTTC CGCAACCATT TGGGCCCCAC GCCCGAAAAA 1501 CTCGCCACCC TGCGCGAGCA GCTCGGTCTG TTGGGGCTTC AACTTGGCGG 1551 CGGCGACAAC CCGTCGCCGA AAGACTATGC CGCGCTTGCC GAACAATTCA 1601 AAGGCAGGCC GGATGCCGAA TTGCTGCAAG TCATGATGTT GCGCTCCATG 1651 CAGCAGGCGG TTTACGAACC GCATTGCGAA GGGCATTTCG GTTTGGCTTA 1701 TGAAGCATAC GCCCACTTTA CCTCGCCCAT CCGCCGCTAT CCCGACCTGA 1751 CCGTCCACCG TGCCATCAAA GCCGTATTGA ACCGGAAAAC CTACACGCCA 1801 AACAAAAGCT GGCAGGCTTT GGGCGTGCAT ACTTCGTTTT GCGAACGCCG 1851 TGCCGACGAT GCTGGCCGCG ATGTGGAAAA CTGGCTGAAA ACTTATTATA 1901 TGCGCGATAA GGTCGGTGAA ATATTTGAAG GcaaaatCtc ccggggtgtg 1951 gcaaaTtttg gaATATTTGT CACTTTGGAC GATATccata tcgacggtct 2001 ggtacaTATC AGCGatttgg gcgaAGATTA TTTCaacttc cgccccgAAA 2051 TCATGGCAAT CGAAGGCGAA CGCAGCGGCA TCCGTTTCAA TATGGGGGAC 2101 AGGGTTGCCG TCCGGGTCGC GCGTGCCGAT TTGGATGATG GAAAAATCGA 2151 CTTTGTCCTA ATTGCCGGAG AAAGCGGCAG GCGGCGGAAG GTCAAATTAT 2201 CCGCATCTGC CAAACCGGCA GGGGCGGCGG GGAAAGGGAA ATCGAAAACC 2251 ACCGCCGAGA AAAAAACAGC CCGATGCGGC AAAGTAAGGG GAAGGGGCGT 2301 GCCTGCCGTT GCCGAATCGG GGAAAAAGGC AAAGAAACCG GTTCCGATTA 2351 AGGTCAAAAA ACGGAAAGGC AAATCATAA This corresponds to the amino acid sequence <SEQ ID 2980; ORF 988.ng>: g988. pep 1 MNKNIKSLNL REKDPFLSRE KQRYEHPLPS REWIIELLER KGVPSKIESL 51 ARELSITEDE YVFFERRLKA MARDGQVLIN RRGAVCAADK LDLVKCRVEA 101 HKDGFGFAVP LMPMDEGDFV LYERQMRGVM HGDTVTVRPA GMDRRGRREG 151 TFLDIVERAQ SKVVGRFYMD RGVAILEPED KRLNQSIVLE PDGVARFKPE 201 SGQVIVGKIE VYPEQNRPAV AKIIEVLGDY ADSGMEIEIA VRKHHLPHRF 251 SEACAKSAKK IPDHVRKSDL KGRVDLCDLP LVTIDGETAR DFDDAVFAEK 301 VGRNYRLWA IADVSHYVRP DDAIDADAQE RSTSVYFPRR MIPMLPENLS 351 NGICSLNPDV ERLCMVCDMV VTYAGNIKEY RFYPAVMRSH ARLTYNQVWK 401 WLSDGIGNPH KAQIDTLYKL FKILQKKRLA RGAVEFESVE TQMIFDDNGK 451 IEKIVPVVRN DAHKLIEECM LAANVCAADF LLKNKHTALF RNHLGPTPEK 501 LATLREQLGL LGLQLGGGDN PSPKDYAALA EQFKGRPDAE LLQVMMLRSM 551 QQAVYEPHCE GHFGLAYEAY AHFTSPIRRY PDLTVHRAIK AVLNRKTYTP 601 NKSWQALGVH TSFCERRADD AGRDVENWLK TYYMRDKVGE IFEGKISRGV 651 ANFGIFVTLD DIHIDGLVHI SDLGEDYFNF RPEIMAIEGE RSGIRFNMGD 701 RVAVRVARAD LDDGKIDFVL IAGESGRRRK VKLSASAKPA GAAGKGKSKT 751 TAEKKTARCG KVRGRGVPAV AESGKKAKKP VPIKVKKRKG KS* The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2981>: m988.seq (partial) 1 ..ACAGTTCTGG ATATTGTCGA ACGCGCGCAA AGCAAAGTGG TCGGCCGTTT 51 CTATATGGAT AGGGGCGTGG CGATTTTGGA GCCGGAAGAC AAGCGTCTGA 101 ACCAAAGCAT CGTATTGGAA CCGGACGGCG TGGCGCGTTT CAAACCTGAA 151 TCCGGTCAGG TCATCGTCGG CGAAATTGAG GTTTATCCTG AGCAAAACCG 201 GCCGGCAGTG GCAAAAATCA TCGAAGTTTT GGGCGATTAT GCCGACAGCG 251 GCATGGAGAT TGAAATTGCC GTGCGCAAGC ATCATTTGCC GCACCAATTC 301 AGTGAAGCGT GTGCCAAAGC TGCGAAAAAA ATTCCCGTCC ATGTACGCAA 351 AAGCGATTTG AAAGGCCGCG TCGATTTGCG CGACCTGCCT TTGGTAACGA 401 TAGACGGCGA AACGGCGCGC GATTTCGACG ACGCGGTGTT TGCCGAAAAA 451 GTCGGACGCA ATTACCGTCT GGTCGTGGCG ATTGCGGATG TCAGCCATTA 501 TGTCCGCCCT GACGATGTGA TTGATGCAGA TGCTCAAGAA CGCAGTACCA 551 GCGTATATTT CCCGCGCCGT GTGATTCCGA TGCTGCCGGA AAACCTGTCT 601 AACGGCATTT GCTCGCTCAA TCCCGATGTC GAGCGTTTGT GTATGGTGTG 651 CGATATGGTC GTTACCTATG CGGGCAATAT CAAAGAATAC CGCTTCTACC 701 CCGCCGTAAT GCGCTCTCAT GCCCGCCTGA CCTACAACCA AGTTTGGAAA 751 TGGATTTCAG ACGGCATCGA CCATCCGTAC AAAGCCCAAA TCGACACCCT 801 TTACAAACTC TTCAAAATCC TTCAGAAAAA GCGTTTCGAA CGCGGCGCGG 851 TGGAGTTTGA AAGCGTCGAA ACCCAGATGA TTTTCGATGA CAACGGCAAA 901 ATCGAAAAAA TCGTCCCCGT TGTCCGCAAC GATGCCCACA AGCTGATTGA 951 AGAATGTATG CTGGCGGCGA ATGTTTGCGC AGCGGATTTC CTGTTGAAAA 1001 ACAAGCATAC GGCTTTGTTC CGCAACCATT TGGGCCCCAC GCCCGAAAAA 1051 CTCGCCACCC TGCGCGAGCA GCTCGGTCTG TTGGGGCTTC AACTTGGCGG 1101 CGGCGACAAC CCGTCGCCGA AAGACTATGC CGCGCTTGTC GAACAATTCA 1151 AAGGCAGACC TGATGCCGAA TTGCTGCAAG TCATGATGTT GCGCTCCATG 1201 CAGCAGGCGG TTTACGAACC GCATTGCGAC GGACACTTTG GTCTTGCCTA 1251 CGAAGCATAC GCCCACTTCA CCTCGCCCAT CCGCCGCTAT CCCGACCTGA 1301 CCGTACACCG CGCCATCAAA GCCGTGTTGA ATCAGCAAAC CTACACGCCA 1351 AAAAAAAGCT GGCAGGCTTT GGGCGTGCAT ACCTCGTTCT GTGAGCGCCG 1401 TGCCGACGAC GCCAGCCGCG ACGTGGAAAA CTGGCTGAAA ACCTATTATA 1451 TGCGCGATAA GGTCGGCGAA GTATTCGAAG GTAAAATCTC CGGCATGACC 1501 AGTTTTGGTA TCTTTGTAAC ACTGGACGGC ATCCACATTG ACGGCTTGGT 1551 GCATATCAGC GATTTGGGCG AAGACTATTT CAACTTCCGC CCCGAAATCA 1601 TGGCAATCGA AGGCGAACGC AGCGGCATCC GTTTCAACAT GGGGGACAGG 1651 GTTGCCGTCC GGGTCGCCCG TGCCGATTTG GATGACGGAA AAATCGATTT 1701 TGTCCTGATT GCCGGGGGGA GCGGCAGGGG GCGGAAAGTT AAATCATCCG 1751 CGTCTGCCAA ACCGGCAGGG ACGGCGGGGA AAGGGAAGCC GAAAACCGCC 1801 GCCGAGAAAA AAACAGCCCG AGGCGGCAAA GTAAGGGGAA GGGGCGCGTC 1851 TGCCGCCGCA GAATCGAGGA AAAAGGCAAA GAAACCGGTT CCGATTAAGG 1901 TAAAAAAACG GAAAGGCAAA TCATAA This corresponds to the amino acid sequence <SEQ ID 2982; ORF 988>: m988.peρ (partial) 1 ..TVLDIVERAQ SKVVGRFYMD RGVAILEPED KRLNQSIVLE PDGVARFKPE 51 SGQVIVGEIE VYPEQNRPAV AKIIEVLGDY ADSGMEIEIA VRKHHLPHQF 101 SEACAKAAKK IPVHVRKSDL KGRVDLRDLP LVTIDGETAR DFDDAVFAEK 151 VGRNYRLWA IADVSHYVRP DDVIDADAQE RSTSVYFPRR VIPMLPENLS 201 NGICSLNPDV ERLCMVCDMV VTYAGNIKEY RFYPAVMRSH ARLTYNQVWK 251 WISDGIDHPY KAQIDTLYKL FKILQKKRFE RGAVEFESVE TQMIFDDNGK 301 IEKIVPVVRN DAHKLIEECM LAANVCAADF LLKNKHTALF RNHLGPTPEK 351 LATLREQLGL LGLQLGGGDN PSPKDYAALV EQFKGRPDAE LLQVMMLRSM 401 QQAVYEPHCD GHFGLAYEAY AHFTSPIRRY PDLTVHRAIK AVLNQQTYTP 451 KKSWQALGVH TSFCERRADD ASRDVENWLK TYYMRDKVGE VFEGKISGMT 501 SFGIFVTLDG IHIDGLVHIS DLGEDYFNFR PEIMAIEGER SGIRFNMGDR 551 VAVRVARADL DDGKIDFVLI AGGSGRGRKV KSSASAKPAG TAGKGKPKTA 601 AEKKTARGGK VRGRGASAAA ESRKKAKKPV PIKVKKRKGK S* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae m988/g988 94.2% identity in 642 aa overlap 10 20 30 m988.pep TVLDIVERAQSKWGRFYMDRGVAILEPED I II I II II I II II II II II II II II II I I g988 LYERQMRGVMHGDTVTVRPAGMDRRGRREGTFLDIVERAQSKWGRFYMDRGVAILEPED 130 140 150 160 170 180 40 50 60 70 80 90 m988.pep KRLΝQSIVLEPDGVARFKPESGQVIVGEIEVYPEQΝRPAVAKIIEVLGDYADSGMEIEIA MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM g988 KRLΝQSIVLEPDGVARFKPESGQVIVGKIEVYPEQΝRPAVAKIIEVLGDYADSGMEIEIA 190 200 210 220 230 240 100 110 120 130 140 150 m988. pep VRKHHLPHQFSEACAKAAKKIPVHVRKSDLKGRVDLRDLPLVTIDGETARDFDDAVFAEK I II II II M I II I II I : II I II II II II II I I I II I I II II I I II I II II I II II I II g988 VRKHHLPHRFSEACAKSAKKIPDHVRKSDLKGRVDLCDLPLVTIDGETARDFDDAVFAEK 250 260 270 280 290 300 160 170 180 190 200 210 m988.pep VGRΝYRLWAIADVSHYVRPDDVIDADAQERSTSVYFPRRVIPMLPEΝLSΝGICSLΝPDV I II I II I II II II I II I II I II : II II I II II I II I II II : II II I II II II I II II I II g988 VGRΝYRLWAIADVSHYVRPDDAIDADAQERSTSVYFPRRMIPMLPEΝLSΝGICSLΝPDV 310 320 330 340 350 360 220 230 240 250 260 270 m988.pep ERLCMVCDMWTYAGΝIKEYRFYPAVMRSHARLTYΝQVWKWISDGIDHPYKAQIDTLYKL I II II I II I II II II II II II II II II II II II I II II I II : I II I : I : I I II I I I I II g988 ERLCMVCDMVVTYAGΝIKEYRFYPAVMRSHARLTYΝQVWKWLSDGIGΝPHKAQIDTLYKL 370 380 390 400 410 420 280 290 300 310 320 330 m988, •pep FKILQKKRFERGAVEFESVETQMIFDDΝGKIEKIVPWRΝDAHKLIEECMLAAΝVCAADF II II II II : II II II I II I II II I II I I I II II II II II II I I I I II II II II II II II g988 FKILQKKRLARGAVEFESVETQMIFDDΝGKIEKIVPWRΝDAHKLIEECMLAAΝVCAADF 430 440 450 460 470 480 340 350 360 370 380 390 m988. •Pep LLKΝKHTALFRΝHLGPTPEKLATLREQLGLLGLQLGGGDΝPSPKDYAALVEQFKGRPDAE II II II II II I I II I II II I I I I I I II I II II I II II II II I I I II I II : II II II I II I g988 LLKΝKHTALFRΝHLGPTPEKLATLREQLGLLGLQLGGGDΝPSPKDYAALAEQFKGRPDAE 490 500 510 520 530 540 400 410 420 430 440 450 m988.pep LLQVMMLRSMQQAVYEPHCDGHFGLAYEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTP I II I II II II I II II II II : I II II II II I II I II II II II I I I I II II II II I : : I II I g988 LLQVMMLRSMQQAVYEPHCEGHFGLAYEAYAHFTSPIRRYPDLTVHRAIKAVLNRKTYTP 550 560 570 580 590 600 460 470 480 490 500 509 m988.pep KKSWQALGVHTSFCERRADDASRDVENWLKTYYMRDKVGEVFEGKIS-GMTSFGIFVTLD : II I II II II I II I II II II I : II I II II I II I II II I II : II II I I I : : : I II II II I g988 NKSWQALGVHTSFCERRADDAGRDVENWLKTYYMRDKVGEIFEGKISRGVANFGIFVTLD 610 620 630 640 650 660 510 520 530 540 550 560 569 m988.pep GIHIDGLVHISDLGEDYFNFRPEIMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVL II II II II II II II I II II II II II I II I II II II II II II II I I I II I II I II II I II g988 DIHIDGLVHISDLGEDYFNFRPEIMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVL 670 680 690 700 710 720 570 580 590 600 610 620 629 m988. pep IAGGSGRGRKVKSSASAKPAGTAGKGKPKTAAEKKTARGGKVRGRGASAAAESRKKAKKP III III llll I II I II I I : I II II II : I II I I II IIMMM Mill I II I II g988 IAGESGRRRKVKLSASAKPAGAAGKGKSKTTAEKKTARCGKVRGRGVPAVAESGKKAKKP 730 740 750 760 770 780 630 640 m988. pep VPIKVKKRKGKSX II I I II II II I II g988 VPIKVKKRKGKSX 790 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2983>: a988.seq 1 ATGAATAAAA ATATTAAATC TTTAAATTTA CGGGAAAAAG ACCCGTTTTT 51 AAGTCGTGAA AAACAGCGTT ATGAACATCC TTTGCCCAGT CGGGAATGGA 101 TAATCGAGCT GCTTGAACGT AAAGGCGTAC CATCCAAGAT TGAAGCTTTG 151 GTACGCGAAT TGTCGATTAA GGAAGAAGAG TACGAATTTT TCGAACGTCG 201 TCTGAAGGCG ATGGCGCGGG ACGGTCAGGT TTTAATCAAC CGTCGGGGCG 251 CGGTTTGCGC GGCGGACAAA TTGGATTTGG TCAAATGCCG TGTCAAGGCG 301 CACAAAGACC GCTTCGGTTT CGCCGTGCCG CTCACGCCCG CCAAAGACGG 351 TGATTTTGTC TTGTACGAAC GCCAGATGCG CGGCATTATG CACGGCGATA 401 TTGTCACTGT TCGTCCTGCC GGCATGGACG GTAGGGGCCG CCGCGAAGGG 451 ACGGTTCTGG ATATTGTCGA ACGCGCGCAA AGCAAAGTGG TCGGCCGTTT 501 CTAΝATGGAT AGGGGCGTGG CGATTTTGGA GCCGGAAGAC AAGCGTCTGA 551 ACCAAAGCAT CGTATTGGAA CCGGACGGCG TGGCGCGTTT CAAACCTGAA 601 TCCGGTCAGG TCATCGTCGG CGAAATTGAG GTTTATCCTG AGCAAAACCG 651 GCCGGCAGTG GCAAAAATCA TCGAAGTTTT GGGCGATTAT GCCGACAGCG 701 GCATGGAGAT TGAAATTGCC GTGCGCAAGC ATCATTTGCC GCACCAATTC 751 AGTGAAGCGT GTGCCAAAGC CGCGAAAAAA ATTCCCGACC ATGTACGCAA 801 AAGCGATTTG AAAGGCCGCG TCGATTTGCG CGACCTGCCT TTGGTAACGA 851 TAGACGGCGA AACGGCTCGA GATTTTGACG ATGCGGTGTT TGCCGAGAAA 901 ATCGGACGCA ATTACCGTCT GGTCGTGGCG ATTGCCGATG TCAGCCATTA 951 TGTCCGCCCC GATGACGCTA TCGACACGGA CGCTCAGGAA CGCAGCACCA 1001 GTGTTTACTT CCCGCGCCGC GTGATTCCCA TGTTGCCGGA AAACCTGTCC 1051 AACGGCATCT GCTCGCTCAA TCCTCATGTC GAGCGTTTGT GTGTGGTGTG 1101 CGATATGGTT ATCACTTACG CGGGCAATAT CAAAGAATAC CGCTTCTACC 1151 CCGCCGTGAT GCGCTCTCAT GCCCGCCTGA CCTACAACCA AGTTTGGAAA 1201 TGGCTTTCAG GCGGCATCGA GCATCCGTTC AAAACCCAAA TCGACACGCT 1251 TTACAAACTC TTCAAAATCC TTCAGAAAAA GCGTTTCGAA CGCGGGGCGG 1301 TGGAGTTTGA CAGCATCGAA ACCCAAATGC TTTTCGACGA CAACGGTAAA 1351 ATTGAAAAAA TCGTCCCCGT TGTCCGCAAC GATGCCCACA AGCTGATTGA 1401 AGAATGTATG TTGGCGGCAA ACGTTTGCGC AGCGGATTTT CTGTTGAAAA 1451 ACAAGCATAC CGCATTGTTC CGCAACCATT TGGGGCCCAC GCCCGAAAAA 1501 CTCGCCGCCT TGCGCGAGCA GCTCGGTCTG TTGGGGCTTC AACTTGGCGG 1551 CGGCGACAAC CCGTCGCCGA AAGACTATGC CGCGCTTGCC GGACAGTTCA 1601 AAGGCAGGCC GGATGCCGAA TTGCTGCAAG TCATGATGTT GCGCTCCATG 1651 CAACAGGCGG TTTACGAACC GCATTGCGAC GGACACTTTG GTCTTGCCTA 1701 CGAAGCATAC GCCCACTTCA CCTCGCCCAT CCGCCGCTAT CCCGACCTGA 1751 CCGTACACCG CGCCATCAAA GCCGTGTTGA ATCAGCAAAC CTACACGCCA 1801 AAAAAAAGCT GGCAGGCTTT GGGCGTGCAT ACCTCGTTCT GTGAGCGCCG 1851 TGCCGACGAC GCCAGCCGCG ACGTGGAAAA CTGGCTGAAA ACCTATTATA 1901 TGCGCGATAA GGTCGGCGAA GTATTCGAAG GTAAAATCTC CGGCATGACC 1951 AGTTTTGGTA TCTTTGTAAC ACTGGACGGC ATCCACATTG ACGGCTTGGT 2001 GCATATCAGC GATTTGGGCG AAGACTATTT CAACTTCCGC CCCGAAATCA 2051 TGGCAATCGA AGGCGAACGC AGCGGCATCC GTTTCAACAT GGGGGACAGG 2101 GTTGCCGTCC GGGTCGCCCG TGCCGATTTG GATGACGGAA AAATCGATTT 2151 TGTCCTGATT GCCGGGGGGA GCGGCAGGGG GCGGAAAGTT AAATCATCCG 2201 CGTCTGCCAA ACCGGCAGGG ACGGCGGGGA AAGGGAAGCC GAAAACCGCC 2251 GCCGAGAAAA AAACAGCCCG AGGCGGCAAA GTAAGGGGAA GGGGCGCGTC 2301 TGCCGCCGCA GAATCGAGGA AAAAGGCAAA GAAACCGGTT CCGATTAAGG 2351 TAAAAAAACG GAAAGGCAAA TCATAA This corresponds to the amino acid sequence <SEQ ID 2984; ORF 988. a>: a988 pep 1 MNKNIKSLNL REKDPFLSRE KQRYEHPLPS REWIIELLER KGVPSKIEAL 51 VRELSIKEEE YEFFERRLKA MARDGQVLIN RRGAVCAADK LDLVKCRVKA 101 HKDRFGFAVP LTPAKDGDFV LYERQMRGIM HGDIVTVRPA GMDGRGRREG 151 TVLDIVERAQ SKWGRFXMD RGVAILEPED KRLNQSIVLE PDGVARFKPE 201 SGQVIVGEIE VYPEQNRPAV AKIIEVLGDY ADSGMEIEIA VRKHHLPHQF 251 SEACAKAAKK IPDHVRKSDL KGRVDLRDLP LVTIDGETAR DFDDAVFAEK 301 IGRNYRLWA IADVSHYVRP DDAIDTDAQE RSTSVYFPRR VIPMLPENLS 351 NGICSLNPHV ERLCWCDMV ITYAGNIKEY RFYPAVMRSH ARLTYNQVWK 401 WLSGGIEHPF KTQIDTLYKL FKILQKKRFE RGAVEFDSIE TQMLFDDNGK 451 IEKIVPVVRN DAHKLIEECM LAANVCAADF LLKNKHTALF RNHLGPTPEK 501 LAALREQLGL LGLQLGGGDN PSPKDYAALA GQFKGRPDAE LLQVMMLRSM 551 QQAVYEPHCD GHFGLAYEAY AHFTSPIRRY PDLTVHRAIK AVLNQQTYTP 601 KKSWQALGVH TSFCERRADD ASRDVENWLK TYYMRDKVGE VFEGKISGMT 651 SFGIFVTLDG IHIDGLVHIS DLGEDYFNFR PEIMAIEGER SGIRFNMGDR 701 VAVRVARADL DDGKIDFVLI AGGSGRGRKV KSSASAKPAG TAGKGKPKTA 751 AEKKTARGGK VRGRGASAAA ESRKKAKKPV PIKVKKRKGK S* m988/a988 97.0% identity in 641 aa overlap 10 20 30 m988.pep TVLDIVERAQSKWGRFYMDRGVAILEPED II I I II II I I II II I I I II II I I II I I I I a988 LYERQMRGIMHGDIVTVRPAGMDGRGRREGTVLDIVERAQSKVVGRFXMDRGVAILEPED 130 140 150 160 170 180 40 50 60 70 80 90 m988.pep KRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRPAVAKIIEVLGDYADSGMEIEIA II II I II I II II II II II I II II II II II II I II II II II II II I I II II II I II II I II a988 KRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRPAVAKIIEVLGDYADSGMEIEIA 190 200 210 220 230 240 100 110 120 130 140 150 m988.pep VRKHHLPHQFSEACAKAAKKIPVHVRKSDLKGRVDLRDLPLVTIDGETARDFDDAVFAEK II II II II II II II II I I II II II II II II II II I II II II II II I I II I I II I II II I a988 VRKHHLPHQFSEACAKAAKKIPDHVRKSDLKGRVDLRDLPLVTIDGETARDFDDAVFAEK 250 260 270 280 290 300 160 170 180 190 200 210 m988.pep VGRNYRLWAIADVSHYVRPDDVIDADAQERSTSVYFPRRVIPMLPENLSNGICSLNPDV M II I II I II I II II I II II II : IM I II II II II I II II II II II I I I I II II I! I I I a988 IGRNYRLWAIADVSHYVRPDDAIDTDAQERSTSVYFPRRVIPMLPENLSNGICSLNPHV 310 320 330 340 350 360 220 230 240 250 260 270 m988. pep ERLCMVCDMWTYAGNIKEYRFYPAVMRSHARLTYNQVWKWISDGIDHPYKAQIDTLYKL II I M I I I II : I I II I I I I I I I I I I I II II II I I I II I I I M I I M I I: I : I I I I I I M a988 ERLCWCDMVITYAGNIKEYRFYPAVMRSHARLTYNQVWKWLSGGIEHPFKTQIDTLYKL 370 380 390 400 410 420 280 290 300 310 320 330 m988. pep FKILQKKRFERGAVEFESVETQMIFDDNGKIEKIVPWRNDAHKLIEECMLAANVCAADF I II II II II II II I I I : M II I I : I II I I I II II II II II II I II II I II II II I I I I I I a988 FKILQKKRFERGAVEFDSIETQMLFDDNGKIEKIVPWRNDAHKLIEECMLAANVCAADF 430 440 450 460 470 480 340 350 360 370 380 390 m988.pep LLKNKHTALFRNHLGPTPEKLATLREQLGLLGLQLGGGDNPSPKDYAALVEQFKGRPDAE I II II II II II II II II II II I M II II I II II II II I I II I II II I I I : II II I II II a988 LLKNKHTALFRNHLGPTPEKLAALREQLGLLGLQLGGGDNPSPKDYAALAGQFKGRPDAE 490 500 510 520 530 540 400 410 420 430 440 450 m988.pep LLQVMMLRSMQQAVYEPHCDGHFGLAYEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTP I II I I II II II II I II II II II I II II II I II II II I II II I II II I I II II I II II I I I a988 LLQVMMLRSMQQAVYEPHCDGHFGLAYEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTP 550 560 570 580 590 600 460 470 480 490 500 510 m988.pep KKSWQALGVHTSFCERRADDASRDVENWLKTYYMRDKVGEVFEGKISGMTSFGIFVTLDG I II II II II II I II II I I I I II II II I II I II I II I I II I II I II I I II I II I I I I II II a988 KKSWQALGVHTSFCERRADDASRDVENWLKTYYMRDKVGEVFEGKISGMTSFGIFVTLDG 610 620 630 640 650 660 520 530 540 550 560 570 m988.pep IHIDGLVHISDLGEDYFNFRPEIMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLI I I I I II II II II I II II II I I II II II II I II II I II II I I II II II I II II II II II II a988 IHIDGLVHISDLGEDYFNFRPEIMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLI 670 680 690 700 710 720 580 590 600 610 620 630 m988. pep AGGSGRGRKVKSSASAKPAGTAGKGKPKTAAEKKTARGGKVRGRGASAAAEΞRKKAKKPV I II II I II II I II II I I II I I I II II II II II II II II II II I II II I I II II II I II I I a988 AGGSGRGRKVKSSASAKPAGTAGKGKPKTAAEKKTARGGKVRGRGASAAAESRKKAKKPV 730 740 750 760 770 780 640 m.986 •pep PIKVKKRKGKSX I II I II II II II a98£ PIKVKKRKGKSX 790 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 2985>: g989.seq 1 ATGACCCCTT TCACACTGAA AAAAACCGTC CTGCTGCTCG GCACTGCCTT 51 TGCCGCCGCA TCTGTCCACG CATCCGGCTA CCACTTCGGC ACACAGTCGG 101 TCAACGCGCA AAGCACGGCA AATGCCGCCG ACGCGTCGAC CATCTTCTAC 151 AATCCCGCCG GCCTGACCAA ACTCGACAGC AGCCAGATTT CCGTCAACGC 201 CAACATCGTG CTGCCCAGCA TTCATTATGA AGCAGATTCC GCCACCGACT 251 TTACCGGGCT TCCCGTCCAA GGTTCTAAAA ACGGCAAAAT CACCAAAACC 301 ACGGTCGCAC CCCACATTTA CGGCGCATAC AAAGTCAACG ACAATCTGAC 351 CGTGGGCTTG GGCGTGTACG TCCCCTTCGG CTCTGCCACC GAATACGAAA 401 AAGATTCCGT GTTGCGCCAC AACATCAACA AACTCGGTCT GACCAGCATC 451 GCCGTCGAAC CTGTCGCCGC GTGGAAACTC AACGAACGCC ATTCCTTCGG 501 CGCAGGCATC ATCGCCCAAC ATAATTCCGC CGAACTGCGC AAATATGCCG 551 ACTGAGGAAT CCCAAAAAAA GCGCAAATGC TGCAAGCAAC ACCTTCTAAT 601 CCTACTGCCG CTGCTCAAAT CAAGGCCGAC GGACACGCCG ATGTCAAAGG 651 CAGCGATTGG GGCGTCGGCT ACCAACTGGC GTGGATGTGG GACATCAACG 701 ACCGCGCGCG CGTGGGCGTG AACTACCGTT CCAAAGTTTC ACACACGCTC 751 AAAGGCGATG CCGAATGGGC GGCAGACGGC GCGGCGGCGA AACAACAGTG 801 GAATGACAAT ATGCTCACAC CGCTCGGTTA CACGGCGAAT GAAAAAGCCA 851 GTGTCAAAAT CGTAACGCCT GAGTCTTTGT CCGTACACGG CATGTACAAA 901 GTGTCCGACA AAGCCGACCT GTTCGGCGAC GTAACTTGGA CGCGCCACAG 951 CCGCTTCAAT AAGGCGGAAC TGTTTTTTGA AAAAGAAAAA AATATTGCTA 1001 ATGGCAAAAA ATCCGACCGC ACCACCATCA CCCCCAACTG GCGCAACACC 1051 TACAAAGTCG GCTTGGGCGG TTCTTATCAA ATCAGCGAAC CGCTGCAACT 1101 GCGCGTCGGC ATCGCTTTTG ACAAACCGCC TGTCCGCAAC GCCGACTacC 1151 GCATGAACAG CCTGCCCGAC GGCAACCGCA TCTGGTTCTC CGCCGGCATG 1201 AAATACCATA TCGGCAAAAA CCACGTCGTC GATGCCGCCT ACACCCACAT 1251 CCACATCAAC GACACCAGCT ACCGCACGGC GAAGGCAAGC GGCAACGATG 1301 TGGACAGCAA AGGTGCGTCT TGCGCACGTT TCAAAAACCA CGCCGACATC 1351 ATCGGCCTGC AATACACCTA CAAATTCAAA TAA This corresponds to the amino acid sequence <SEQ ID 2986; ORF 989.ng>: g989.pep 1 MTPFTLKKTV LLLGTAFAAA SVHASGYHFG TQSVNAQSTA NAADASTIFY 51 NPAGLTKLDS SQISVNANIV LPSIHYEADS ATDFTGLPVQ GSKNGKITKT 101 TVAPHIYGAY KVNDNLTVGL GVYVPFGSAT EYEKDSVLRH NINKLGLTSI 151 AVEPVAAWKL NERHSFGAGI IAQHNSAELR KYAD*GIPKK AQMLQATPSN 201 PTAAAQIKAD GHADVKGSDW GVGYQLAWMW DINDRARVGV NYRSKVSHTL 251 KGDAEWAADG AAAKQQWNDN MLTPLGYTAN EKASVKIVTP ESLSVHGMYK 301 VSDKADLFGD VTWTRHSRFN KAELFFEKEK NIANGKKSDR TTITPNWRNT 351 YKVGLGGSYQ ISEPLQLRVG IAFDKPPVRN ADYRMNSLPD GNRIWFSAGM 401 KYHIGKNHW DAAYTHIHIN DTSYRTAKAS GNDVDSKGAS CARFKNHADI 451 IGLQYTYKFK * The following partial DNA sequence was identified in N meningitidis <SEQ ID 2987>: m989. seq 1 ATGACCCCTT CCGCACTGAA AAAAACCGTC CTGCTGCTCG GCACTGCCTT 51 TGCCGCCGCA TCCGTCCACG CATCCGGCTA CCACTTCGGC ACACAGTCGG 101 TCAACGCGCA AAGCACGGCA AATGCCGCCG CCGCAGAAGC CGCCGACGCA 151 TCGACCATCT TCTACAACCC TGCCGGCCTG ACCAAACTCG ACAGCAGCCA 201 GATTTCCGTC AACGCCAACA TCGTGCTGCC CAGCATTCAT TATGAGGCGG 251 ATTCCGCCAC CGACTTTACC GGGCTTCCCG TCCAAGGTTC GAAAAGCGGC 301 AAAATCACCA AAACCACGGT CGCGCCCCAC ATCTACGGCG CATACAAAGT 351 CAACGACAAT CTGACCGTGG GCTTGGGCGT GTACGTCCCC TTCGGCTCTG 401 CCACCGAATA CGAAAAAGAT TCCGTGTTGC GCCACAACAT CAACAAACTC 451 GGTCTGACCA GCATCGCCGT CGAACCTGTC GCCGCGTGGA AACTCAACGA 501 CCGCCATTCC TTCGGCGCAG GCATCATCGC CCAACATACT TCCGCCGAAC 551 TGCGCAAATA TGCCGACTGG GGGATTAAGA GTAAAGCAGA GATATTGACG 601 GCAAAACCGC CCAAACCTAA CGGTGTAGCC GAAGCTGCAA AAATTCAGGC 651 CGACGGACAC GCCGATGTCA AAGGCAGCGA TTGGGGCTTC GGCTACCAAC 701 TGGCGTGGAT GTGGGACATC AACGACCGTG CGCGCGTGGG CGTGAACTAC 751 CGTTCCAAAG TCTCGCACAC GCTCAAAGGC GATGCCGAAT GGGCGGCAGA 801 CGGCGCGGCG GCGAAAGCAA TGTGGAGTAC GATGCTTGCA GCAAACGGCT 851 ACACGGCGAA TGAAAAAGCC CGCGTTAAAA TCGTTACGCC TGAGTCTTTG 901 TCCGTACACG GTATGTACAA AGTGTCCGAT AAAGCCGACC TGTTCGGCGA 951 CGTAACTTGG ACGCGCCACA GCCGCTTCGA TAAGGCGGAA CTGGTTTTTG 1001 AAAAAGAAAA AACCGTCGTC AAAGGCAAAT CCGACCGCAC CACCATCACC 1051 CCCAACTGGC GCAACACCTA CAAAGTCGGC TTCGGCGGTT CTTATCAAAT 1101 CAGCGAACCG CTGCAACTGC GCGCCGGCAT CGCTTTTGAC AAATCGCCCG 1151 TCCGCAACGC CGACTACCGC ATGAACAGCC TACCCGACGG CAACCGCATC 1201 TGGTTCTCCG CCGGTATGAA ATACCATATC GGTAAAAACC ACGTCGTCGA 1251 TGCCGCCTAC ACCCACATCC ACATCAACGA CACCAGCTAC CGCACGGCGA 1301 AGGCAAGCGG CAACGATGTG GACAGCAAAG GCGCGTCTTC CGCACGTTTC 1351 AAAAACCACG CCGACATCAT CGGTCTGCAA TACACCTACA AATTCAAATA 1401 A This corresponds to the amino acid sequence <SEQ ID 2988; ORF 989>: m98 .pep 1 MTPSALKKTV LLLGTAFAAA SVHASGYHFG TQSVNAQSTA NAAAAEAADA 51 STIFYNPAGL TKLDSSQISV NANIVLPSIH YEADSATDFT GLPVQGSKSG 101 KITKTTVAPH IYGAYKVNDN LTVGLGVYVP FGSATEYEKD SVLRHNINKL 151 GLTSIAVEPV AAWKLNDRHS FGAGIIAQHT SAELRKYADW GIKSKAEILT 201 AKPPKPNGVA EAAKIQADGH ADVKGSDWGF GYQLAWMWDI NDRARVGVNY 251 RSKVSHTLKG DAEWAADGAA AKAMWSTMLA ANGYTANEKA RVKIVTPESL 301 SVHGMYKVSD KADLFGDVTW TRHSRFDKAE LVFEKEKTW KGKSDRTTIT 351 PNWRNTYKVG FGGSYQISEP LQLRAGIAFD KSPVRNADYR MNSLPDGNRI 401 WFSAGMKYHI GKNHWDAAY THIHINDTSY RTAKASGNDV DSKGASSARF 451 KNHADIIGLQ YTYKFK* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae g989/m989 90.0% identity in 468 aa overlap 10 20 30 40 50 g989.pep MTPFTLKKTVLLLGTAFAAASVHASGYHFGTQSVΝAQSTAΝAA DASTIFYΝPAGL III : I II II I II II I II II II II II II I II II II II II II I I I I I I II I II I II m989 MTPSALKKTVLLLGTAFAAASVHASGYHFGTQSVΝAQSTAΝAAAAEAADASTIFYΝPAGL 10 20 30 40 50 60 60 70 80 90 100 110 g989.pep TKLDSSQISVΝAΝIVLPSIHYEADSATDFTGLPVQGSKΝGKITKTTVAPHIYGAYKVΝDΝ I I I I I I II II II I II II II II II I II II II I II II II I : II II II II I II I I II I II II I m989 TKLDSSQISVΝAΝIVLPSIHYEADSATDFTGLPVQGSKSGKITKTTVAPHIYGAYKVΝDΝ 70 80 90 100 110 120 120 130 140 150 160 170 g989.pep LTVGLGVYVPFGSATEYEKDSVLRHΝIΝKLGLTSIAVEPVAAWKLΝERHΞFGAGIIAQHΝ I II II II II II II II I II I II II I II I II II II I II II II II II I I : I II II II I II I M m989 LTVGLGVYVPFGSATEYEKDSVLRHΝIΝKLGLTSIAVEPVAAWKLΝDRHSFGAGIIAQHT 130 140 150 160 170 180 180 190 200 210 220 230 g989. pep SAELRKYADXGIPKKAQMLQATPSΝPTA AAQIKADGHADVKGSDWGVGYQLAWMWDI II II II I II II : \ \ : : \ I I : I : : II : I : II II II II I II I I llllllllll m989 SAELRKYADWGIKSKAEILTAKPPKPΝGVAEAAKIQADGHADVKGSDWGFGYQLAWMWDI 190 200 210 220 230 240 240 250 260 270 280 290 g989. pep ΝDRARVGVΝYRSKVSHTLKGDAEWAADGAAAKQQWΝDΝMLTPLGYTAΝEKASVKIVTPES I II II I II II II II II II II II II II II I II I : I : MM llllllll IIIIIMI m989 ΝDRARVGVΝYRSKVSHTLKGDAEWAADGAAAKAMWS-TMLAAΝGYTAΝEKARVKIVTPES 250 260 270 280 290 300 310 320 330 340 350 g989.pep LSVHGMYKVSDKADLFGDVTWTRHSRFΝKAELFFEKEKΝIAΝGKKSDRTTITPΝWRΝTYK II I II II II II II II I II I II II I II I : I I II II II I :: :: I I MMMMIMMM m989 LSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFEKEKTWKGK-SDRTTITPΝWRΝTYK 300 310 320 330 340 350 360 370 380 390 400 410 g989. pep VGLGGSYQISEPLQLRVGIAFDKPPVRΝADYRMΝSLPDGΝRIWFSAGMKYHIGKΝHWDA I I: I II I I I II I II I I : II II II II II II II II II II II II II I I II II II II I II II I m989 VGFGGSYQISEPLQLRAGIAFDKSPVRΝADYRMΝSLPDGΝRIWFSAGMKYHIGKΝHWDA 360 370 380 390 400 410 420 430 440 450 460 g989.pep AYTHIHIΝDTSYRTAKASGΝDVDSKGASCARFKΝHADIIGLQYTYKFKX II I II II I I II II II I II I II II II I II II II II II II II II II II I I m989 AYTHIHIΝDTSYRTAKASGΝDVDSKGASSARFKΝHADIIGLQYTYKFKX 420 430 440 450 460 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2989>: a989.seq 1 ATGACCCCTT CCGCACTGAA AAAAACCGTC CTACTGCTCG GCACTGCCTT 51 TGCCGCCGCA TCCGCACAAG CCTCCGGCTA CCACTTCGGC ACACAGTCGG 101 TCAACGCGCA AAGCACGGCA AATGCCGCCG CCGCAGAAGC CGCCGACGCA 151 TCGACCATCT TCTACAACCC TGCCGGCCTG ACCAAACTCG ACAGCAGCCA 201 GATTTCCGTC AACGCCAACA TCGTGCTGCC CAGCATTCAT TATGAGGCGG 251 ATTCCGCCAC CGACTTTACC GGGCTTCCCG TCCAAGGTTC GAAAAGCGGC 301 AAAATCACCA AAACCACGGT CGCGCCCCAC ATCTACGGCG CATACAAAGT 351 CAACGACAAT CTGACCGTAG GCTTGGGCGT GTACGTCCCC TTCGGTTCTG 401 CCACCGAATA CGAAAAAGAT TCCGTGTTGC GCCACAACAT CAACAAACTC 451 GGTCTGACCA GCATCGCCGT CGAACCTGTC GCCGCGTGGA AACTCAACGA 501 ACGCCATTCC TTCGGCGCAG GCATCATCGC CCAACATACT TCCGCCGAGC 551 TGCGCAAATA TGCCGACTGG GGGATTATGG AAAAAGCGAA AGCACTAAAA 601 GAAACACCCC CCAATCCAAC TAAAGCCGCC CAAATCAAAG CCGACGGACA 651 CGCCGATGTC AAAGGCAGCG ATTGGGGCTT CGGCTACCAA CTGGCGTGGA 701 TGTGGGACAT CAACGACCGT GCGCGCGTGG GCGTGAACTA CCGTTCCAAA 751 GTCTCACACA CGCTCAAAGG CGATGCCGAA TGGGCGGCAG ACGACGCAAT 801 GGCGAAACAG TTATGGGATG CAAACAAACT CGCACTGCTC GGCTACACGC 851 CAAGCGAAAA AGCCCGCGTT AAAATCGTTA CGCCCGAGTC TTTGTCCGTA 901 CACGGTATGT ACAAAGTGTC CGACAAAGCC GACCTGTTCG GCGACGTAAC 951 TTGGACGCGC CACAGCCGCT TCGATAAGGC GGAACTGGTT TTTGAAAAAG 1001 AAAAAACCAT CGTCAACGGC AAATCCGACC GCACCACCAT CACCCCCAAC 1051 TGGCGCAACA CCTACAAAGT CGGCTTCGGC GGTTCTTATC AAATCAGCGA 1101 ACCGCTGCAA CTGCGCGCCG GCATCGCTTT TGACAAATCG CCCGTCCGCA 1151 ACGCCGACTA CCGCATGAAC AGCCTGCCCG ACGGCAACCG CATCTGGTTC 1201 TCCGCCGGCA TGAAATACCA TATCGGCAAA AACCACGTCG TCGATGCCGC 1251 CTACACCCAC ATCCACATCA ACGACACCAG CTACCGCACG GCGAAGGCAA 1301 GCGGCAACGA TGTGGACAGC AAAGGCGCGT CTTCCGCACG TTTCAAAAAC 1351 CACGCCGACA TCATCGGCCT GCAATACACC TACAAATTCA AATAA This corresponds to the amino acid sequence <SEQ ID 2990; ORF 989.a>: a989.pep 1 MTPSALKKTV LLLGTAFAAA SAQASGYHFG TQSVNAQSTA NAAAAEAADA 51 STIFYNPAGL TKLDSSQISV NANIVLPSIH YEADSATDFT GLPVQGSKSG 101 KITKTTVAPH IYGAYKVNDN LTVGLGVYVP FGSATEYEKD SVLRHNINKL 151 GLTSIAVEPV AAWKLNERHS FGAGIIAQHT SAELRKYADW GIMEKAKALK 201 ETPPNPTKAA QIKADGHADV KGSDWGFGYQ LAWMWDINDR ARVGVNYRSK 251 VSHTLKGDAE WAADDAMAKQ LWDANKLALL GYTPSEKARV KIVTPESLSV 301 HGMYKVSDKA DLFGDVTWTR HSRFDKAELV FEKEKTIVNG KSDRTTITPN 351 WRNTYKVGFG GSYQISEPLQ LRAGIAFDKS PVRNADYRMN SLPDGNRIWF 401 SAGMKYHIGK NHWDAAYTH IHINDTSYRT AKASGNDVDS KGASSARFKN 451 HADIIGLQYT YKFK* m989/a989 93.1% identity in 467 aa overlap 10 20 30 40 50 60 m989. pep MTPSALKKTVLLLGTAFAAASVHASGYHFGTQSVNAQSTANAAAAEAADASTIFYNPAGL I II II I I I II II I II II I II I : : I II II II II II II II I II II I II I I II I I II II II I I a989 MTPSALKKTVLLLGTAFAAASAQASGYHFGTQSVNAQSTANAAAAEAADASTIFYNPAGL 10 20 30 40 50 60 70 80 90 100 110 120 m989.pep TKLDSSQISVNANIVLPSIHYEADSATDFTGLPVQGSKSGKITKTTVAPHIYGAYKVNDN II II II II I II I II II II II II II II II II I II II II II II II I II I II I II I I II II II a989 TKLDSSQISVNANIVLPSIHYEADSATDFTGLPVQGSKSGKITKTTVAPHIYGAYKVNDN 70 80 90 100 110 120 130 140 150 160 170 180 m989.pep LTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPVAAWKLNDRHSFGAGIIAQHT I II II II II II II II II I II II II II II II II II II II II II I II I : II II I II I II I I I a989 LTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPVAAWKLNERHSFGAGIIAQHT 130 140 150 160 170 180 190 200 210 220 230 240 m989.pep SAELRKYADWGIKSKAEILTAKPPKPNGVAEAAKIQADGHADVKGSDWGFGYQLAWMWDI I II II II II I II IM I IMM : I I : I : II I II I II II I II II I I II II I II a989 SAELRKYADWGIMEKAKALKETPPNPT KAAQIKADGHADVKGSDWGFGYQLAWMWDI 190 200 210 220 230 250 260 270 280 290 299 m989. pep NDRARVGVNYRSKVSHTLKGDAEWAADGAAAKAMW-STMLAANGYTANEKARVKIVTPES II II II II II II II I II II II II I I II I II M :: II III : || I II II I II II a989 NDRARVGVNYRSKVSHTLKGDAEWAADDAMAKQLWDANKLALLGYTPSEKARVKIVTPES 240 250 260 270 280 290 300 310 320 330 340 350 359 m989.pep LSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFEKEKTWKGKSDRTTITPNWRNTYKV MMMMMMMMMMMMMMMMMMMMMMMMMMMMMM a989 LSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFEKEKTIVNGKSDRTTITPNWRNTYKV 300 310 320 330 340 350 360 370 380 390 400 410 419 m989.pep GFGGSYQISEPLQLRAGIAFDKSPVRNADYRMNSLPDGNRIWFSAGMKYHIGKNHWDAA I II II II II II II II II II II II I II II II II II II I II I II II II I I II I II II II II I a989 GFGGSYQISEPLQLRAGIAFDKSPVRNADYRMNSLPDGNRIWFSAGMKYHIGKNHWDAA 360 370 380 390 400 410 420 430 440 450 460 m989.pep YTHIHINDTSYRTAKASGNDVDSKGASSARFKNHADIIGLQYTYKFKX II I II I II II II I II II I I II I I II II II II II I II I II II I II II I I a989 YTHIHINDTSYRTAKASGNDVDSKGASSARFKNHADIIGLQYTYKFKX 420 430 440 450 460 The following partial DNA sequence was identified in N meningitidis <SEQ ID 2991>: m990. seq 1 ATGTTCAGAG CTCAGCTTGG TTCAAATACT CGTTCTACCA AAATCGGCGA 51 CGATGCCGAT TTTTCATTTT CAGACAAGCC GAAACCCGGC ACTTCCCATT 101 ATTTTTCCAG CGGTAAAACC GATCAAAATT CATCCGAATA TGGGTATGAC 151 GAAATCAATA TCCAAGGTAA AAATTACAAT AGCGGCATCC TCGCCGTCGA 201 TAATATGCCC GTTGTCAAAA AATATATTAC AGAGAAGTAT GGGGCTGATT 251 TAAAGCAGGC GGTTAAAAGT CAATTACAGG ATTTATACAA AACAAGACCG 301 GAAGCTTGGG CAGAAAATAA AAAACGGACT GAGGAGGCGT ATATAGCACA 351 GTTTGGAACA AAATTTAGTA CGCTCAAACA GACGATGCCC GATTTAATTA 401 ATAAATTGGT AGAAGATTCC GTACTCACTC CTCATAGTAA TACATCACAG 451 ACTAGTCTCA ACAACATCTT CAATAAAAAA TTACACGTCA AAATCGAAAA 501 CAAATCCCAC GTCGCCGGAC AGGTGTTGGA ACTGACCAAG ATGACGCTGA 551 AAGATTCCCT TTGGGAACCG CGCCGCCATT CCGACATCCA TACGCTGGAA 601 ACTTCCGATA ATGCCCGCAT CCGCCTGAAC ACGAAAGATG AAAAACTGAC 651 CGTCCATAAG GATTATGCGG GCGGCGCGGA TTTCCTGTTC GGCTACGACG 701 TGCGGGAGTC GGACGAACCC GCCCTGACCT TTGAAGACAA AGTCAGCGGA 751 CAATCCGGCG TGGTTTTGGA ACGCCGGCCG GAAAATCTGA AAACGCTCGA 801 CGGGCGCAAA CTGATTGCGG CAAAAACGGC GGATTCCGGT TCGTTTGCGT 851 TTAAACAAAA TTACCGGCAG GGACTGTACG AATTATTGCT CAAGCAATGC 901 GAAGGCGGAT TTTGCTTGGG CGTGCAGCGT TTGGCTATCC CCGAGGCGGA 951 AGCGGTTTTA TATGCCCAAC AGGCTTATGC GGCAAATACT TTGTTTGGGC 1001 TGCGTGCCGC CGACAGGGGC GACGACGTGT ATGCCGCCGA TCCGTCCCGT 1051 CAAAAATTGT GGCTGCGCTT CATCGGCGGC CGGTCGCATC AAAATATACG 1101 GGGCGGCGCG GCTGCGGACG GGTGGCGCAA AGGCGTGCAA ATCGGCGGCG 1151 AGGTGTTTGT ACGGCAAAAT GAAGGCAGCC GACTGGCAAT CGGCGTGATG 1201 GGCGGCAGGG CCGGCCAGCA CGCATCAGTC AACGGCAAAG GCGGTGCGGC 1251 AGGCAGTGAT TTGTATGGTT ATGGCGGGGG TGTTTATGCT GCGTGGCATC 1301 AGTTGCGCGA TAAACAAACG GGTGCGTATT TGGACGGCTG GTTGCAATAC 1351 CAACGTTTCA AACACCGCAT CAATGATGAA AACCGTGCGG AACGCTACAA 1401 AACCAAAGGT TGGACGGCTT CTGTCGAAGG CGGCTACAAC GCGCTTGTGG 1451 CGGAAGGCAT TGTCGGAAAA GGCAATAATG TGCGGTTTTA CCTACAACCG 1501 CAGGCGCAGT TTACCTACTT GGGCGTAAAC GGCGGCTTTA CCGACAGCGA 1551 GGGGACGGCG GTCGGACTGC TCGGCAGCGG TCAGTGGCAA AGCCGCGCCG 1601 GCATTCGGGC AAAAACCCGT TTTGCTTTGC GTAACGGTGT CAATCTTCAG 1651 CCTTTTGCCG CTTTTAATGT TTTGCACAGG TCAAAATCTT TCGGCGTGGA 1701 AATGGACGGC GAAAAACAGA CGCTGGCAGG CAGGACGGCA CTCGAAGGGC 1751 GGTTCGGTAT TGAAGCCGGT TGGAAAGGCC ATATGTCCGC ACGCATCGGA 1801 TATGGCAAAA GGACGGACGG CGACAAAGAA GCCGCATTGT CGCTCAAATG 1851 GCTGTTTTGA This corresponds to the amino acid sequence <SEQ ID 2992; ORF 990>: m990 . pep 1 MFRAQLGSNT RSTKIGDDAD FSFSDKPKPG TSHYFSSGKT DQNSSEYGYD 51 EINIQGKNYN SGILAVDNMP WKKYITEKY GADLKQAVKS QLQDLYKTRP 101 EAWAENKKRT EEAYIAQFGT KFSTLKQTMP DLINKLVEDS VLTPHSNTSQ 151 TSLNNIFNKK LHVKIENKSH VAGQVLELTK MTLKDSLWEP RRHSDIHTLE 201 TSDNARIRLN TKDEKLTVHK DYAGGADFLF GYDVRESDEP ALTFEDKVSG 251 QSGWLERRP ENLKTLDGRK LIAAKTADSG SFAFKQNYRQ GLYELLLKQC 301 EGGFCLGVQR LAIPEAEAVL YAQQAYAANT LFGLRAADRG DDVYAADPSR 351 QKLWLRFIGG RSHQNIRGGA AADGWRKGVQ IGGEVFVRQN EGSRLAIGVM 401 GGRAGQHASV NGKGGAAGSD LYGYGGGVYA AWHQLRDKQT GAYLDGWLQY 451 QRFKHRINDE NRAERYKTKG WTASVEGGYN ALVAEGIVGK GNNVRFYLQP 501 QAQFTYLGVN GGFTDSEGTA VGLLGSGQWQ SRAGIRAKTR FALRNGVNLQ 551 PFAAFNVLHR SKSFGVEMDG EKQTLAGRTA LEGRFGIEAG WKGHMSARIG 601 YGKRTDGDKE AALSLKWLF* The following partial DNA sequence was identified in N meningitidis <SEQ ID 2993>: a990.seq 1 ATGTTCAGAG CTCAGCTTGG TTCAAATACT CGTTCTACCA AAATCGGCGA 51 CGATGCCGAT TTTTCATTTT CAGACAAGCC GAAACCCGGC ACTTCCCATT 101 ATTTTTCCAG CGGTAAAACC GATCAAAATT CATCCGAATA TGGGTATGAC 151 GAAATCAATA TCCAAGGTAA AAACTACAAT AGCGGCATAC TCGCCGTCGA 201 TAATATGCCC GTTGTTAAGA AATATATTAC AGATACTTAC GGGGATAATT 251 TAAAGGATGC GGTTAAGAAG CAATTACAGG ATTTATACAA AACAAGACCC 301 GAAGCTTGGG AAGAAAATAA AAAACGGACT GAGGAGGCGT ATA AGAACA 351 GCTTGGACCA AAATTTAGTA TACTCAAACA GAAAAACCCC GATTTAATTA 401 ATAAATTGGT AGAAGATTCC GTACTCACTC CTCATAGTAA TACATCACAG 451 ACTAGTCTCA ACAACATCTT CAATAAAAAA TTACACGTCA AAATCGAAAA 501 CAAATCCCAC GTCGCCGGAC AGGTGTTGGA ACTGACCAAG ATGACGCTGA 551 AAGATTCCCT TTGGGAACCG CGCCGCCATT CCGACATCCA TATGCTGGAA 601 ACTTCCGATA ATGCCCGCAT CCGCCTGAAC ACGAAAGATG AAAAACTGAC 651 CGTCCATAAA GCGTATCAGG GCGGTGCGGA TTTCCTGTTC GGCTACGACG 701 TGCGGGAGTC GGACAAACCC GCCCTGACCT TTGAAGAAAA AGTCAGCGGA 751 CAATCCGGCG TGGTTTTGGA ACGCCGGCCG GAAAATCTGA AAACGCTCGA 801 CGGGCGCAAA CTGATTGCGG CGGAAAAGGC AGACTCTAAT TCGTTTGCGT 851 TTAAACAAAA TTACCGGCAG GGACTGTACG AATTATTGCT CAAGCAATGC 901 GAAGGCGGAT TTTGCTTGGG CGTGCAGCGT TTGGCTATCC CCGAGGCGGA 951 AGCGGTTTTA TATGCCCAAC AGGCTTATGC GGCAAATACT TTGTTCGGGC 1001 TGCGTGCCGC CGACAGGGGC GACGACGTGT ATGCCGCCGA TCCGTCCCGT 1051 CAAAAATTGT GGCTGCGCTT CATCGGCGGC CGGTCGCATC AAAATATACG 1101 GGGCGGCGCG GCTGCGGACG GGCGGCGCAA AGGCGTGCAA ATCGGCGGCG 1151 AGGTGTTTGT ACGGCAAAAT GAAGGCAGCC GGCTGGCAAT CGGCGTGATG 1201 GGCGGCAGGG CTGGCCAGCA CGCATCAGTC AACGGCAAAG GCGGTGCGGC 1251 AGGCAGTTAT TTGCATGGTT ATGGCGGGGG TGTTTATGCT GCGTGGCATC 1301 AGTTGCGCGA TAAACAAACG GGTGCGTATT TGGACGGCTG GTTGCAATAC 1351 CAACGTTTCA AACACCGCAT CAATGATGAA AACCGTGCGG AACGCTACAA 1401 AACCAAAGGT TGGACGGCTT CTGTCGAAGG CGGCTACAAC GCGCTTGTGG 1451 CGGAAGGCGT TGTCGGAAAA GGCAATAATG TGCGGTTTTA CCTGCAACCG 1501 CAGGCGCAGT TTACCTACTT GGGCGTAAAC GGCGGCTTTA CCGACAGCGA 1551 GGGGACGGCG GTCGGACTGC TCGGCAGCGG TCAGTGGCAA AGCCGCGCCG 1601 GCATTCGGGC AAAAACCCGT TTTGCTTTGC GTAACGGTGT CAATCTTCAG 1651 CCTTTTGCCG CTTTTAATGT TTTGCACAGG TCAAAATCTT TCGGCGTGGA 1701 AATGGACGGC GAAAAACAGA CGCTGGCAGG CAGGACGGCG CTCGAAGGGC 1751 GGTTCGGCAT TGAAGCCGGT TGGAAAGGCC ATATGTCCGC ACGCATCGGA 1801 TACGGCAAAA GGACGGACGG CGACAAAGAA GCCGCATTGT CGCTCAAATG 1851 GCTGTTTTGA This corresponds to the amino acid sequence <SEQ ID 2994; ORF 990. a>: a990.pep 1 MFRAQLGSNT RSTKIGDDAD FSFSDKPKPG TSHYFSSGKT DQNSSEYGYD 51 EINIQGKNYN SGILAVDNMP WKKYITDTY GDNLKDAVKK QLQDLYKTRP 101 EAWEENKKRT EEAYIEQLGP KFSILKQKNP DLINKLVEDS VLTPHSNTSQ 151 TSLNNIFNKK LHVKIENKSH VAGQVLELTK MTLKDSLWEP RRHSDIHMLE 201 TSDNARIRLN TKDEKLTVHK AYQGGADFLF GYDVRESDKP ALTFEEKVSG 251 QSGWLERRP ENLKTLDGRK LIAAEKADSN SFAFKQNYRQ GLYELLLKQC 301 EGGFCLGVQR LAIPEAEAVL YAQQAYAANT LFGLRAADRG DDVYAADPSR 351 QKLWLRFIGG RSHQNIRGGA AADGRRKGVQ IGGEVFVRQN EGSRLAIGVM 401 GGRAGQHASV NGKGGAAGSY LHGYGGGVYA AWHQLRDKQT GAYLDGWLQY 451 QRFKHRINDE NRAERYKTKG WTASVEGGYN ALVAEGVVGK GNNVRFYLQP 501 QAQFTYLGVN GGFTDSEGTA VGLLGSGQWQ SRAGIRAKTR FALRNGVNLQ 551 PFAAFNVLHR SKSFGVEMDG EKQTLAGRTA LEGRFGIEAG WKGHMSARIG 601 YGKRTDGDKE AALSLKWLF* m990/a990 96.0% identity in 619 aa overlap 10 20 30 40 50 60 m990.pep MFRAQLGSNTRSTKIGDDADFSFSDKPKPGTSHYFSSGKTDQNSSEYGYDEINIQGKNYN I II II II I II II II II II II II II II II II II II II II II II I I I II II I I II I II II II a990 MFRAQLGSNTRSTKIGDDADFSFSDKPKPGTSHYFSSGKTDQNSSEYGYDEINIQGKNYN 10 20 30 40 50 60 70 80 90 100 110 120 m990. pep SGILAVDNMPVVKKYITEKYGADLKQAVKSQLQDLYKTRPEAWAENKKRTEEAYIAQFGT I I II I II II II II I II I : II : II : II M II II I II II II II II I II II I I II I : I a990 SGILAVDNMPVVKKYITDTYGDNLKDAVKKQLQDLYKTRPEAWEENKKRTEEAYIEQLGP 70 80 90 100 110 120 130 140 150 160 170 180 m990.pep KFSTLKQTMPDLINKLVEDSVLTPHSNTSQTSLNNIFNKKLHVKIENKSHVAGQVLELTK Ml III II II II II II II II II II II II II II II II II I II I I I I II I I I II II II I a990 KFSILKQKNPDLINKLVEDSVLTPHSNTSQTSLNNIFNKKLHVKIENKSHVAGQVLELTK 130 140 150 160 170 180 190 200 210 220 230 240 m990.pep MTLKDSLWEPRRHSDIHTLETSDNARIRLNTKDEKLTVHKDYAGGADFLFGYDVRESDEP II II I II II II II II II I II II II I II II II II II II II I II I I II II II II II I : I a990 MTLKDSLWEPRRHSDIHMLETSDNARIRLNTKDEKLTVHKAYQGGADFLFGYDVRESDKP 190 200 210 220 230 240 250 260 270 280 290 300 m990. pep ALTFEDKVSGQSGWLERRPENLKTLDGRKLIAAKTADSGSFAFKQNYRQGLYELLLKQC I II II : I II II II II II II II I I II II II II II I: II I : I II II II II II II II I II II a990 ALTFEEKVSGQSGWLERRPENLKTLDGRKLIAAEKADSNSFAFKQNYRQGLYELLLKQC 250 260 270 280 290 300 310 320 330 340 350 360 m990.pep EGGFCLGVQRLAIPEAEAVLYAQQAYAANTLFGLRAADRGDDVYAADPSRQKLWLRFIGG I I I II II II II II II II II II II II II II II I II II II II II II II I II II II II II II I a990 EGGFCLGVQRLAIPEAEAVLYAQQAYAANTLFGLRAADRGDDVYAADPSRQKLWLRFIGG 310 320 330 340 350 360 370 380 390 400 410 420 m990.pep RSHQNIRGGAAADGWRKGVQIGGEVFVRQNEGSRLAIGVMGGRAGQHASVNGKGGAAGSD II II II II II II II II II II II II II I II II II II I I I I II II II II I II I II II I II a990 RSHQNIRGGAAADGRRKGVQIGGEVFVRQNEGSRLAIGVMGGRAGQHASVNGKGGAAGSY 370 380 390 400 410 420 430 440 450 460 470 480 m990. pep LYGYGGGVYAAWHQLRDKQTGAYLDGWLQYQRFKHRINDENRAERYKTKGWTASVEGGYN I : II II II II I II II II II II II II II II II II I II II II II I II I II II I II I II II II a990 LHGYGGGVYAAWHQLRDKQTGAYLDGWLQYQRFKHRINDENRAERYKTKGWTASVEGGYN 430 440 450 460 470 480 490 500 510 520 530 540 m990. pep ALVAEGIVGKGNNVRFYLQPQAQFTYLGVNGGFTDSEGTAVGLLGSGQWQSRAGIRAKTR I II I I I : II II II II II II II II II II II II II II II I II II II II II II II II II II II a990 ALVAEGWGKGNNVRFYLQPQAQFTYLGVNGGFTDSEGTAVGLLGSGQWQSRAGIRAKTR 490 500 510 520 530 540 550 560 570 580 590 600 m990. pep FALRNGVNLQPFAAFNVLHRSKSFGVEMDGEKQTLAGRTALEGRFGIEAGWKGHMSARIG I II I II II II II II I II II II II II II I II II II II II I II II II I I II II II I II II II a990 FALRNGVNLQPFAAFNVLHRSKSFGVEMDGEKQTLAGRTALEGRFGIEAGWKGHMSARIG 550 560 570 580 590 600 610 620 m990.pep YGKRTDGDKEAALSLKWLFX II II II I II II I II II II II a990 YGKRTDGDKEAALSLKWLFX 610 620 The following partial DNA sequence was identified in N. gonorrhoeae <SEQ ID 2995>: g992.seq 1 ATGTTCAGAC GGCATCGGCA TTTGAAAAAT ATGCAGATTA AAAAAATCAT 51 GAAATGGCTT CCCGTCGCCC TGTCGCTTTT GGGCGCGTTG GGTTATACGG 101 GATATGACAG TGAGGCGGTG CGGACGGCGG TTGCCGTACT CGACGTACTC 151 GGCACTGCAG GGGACGTGGG TTTCGACGCG CCCGTTCGCC GACGGGCATC 201 GGCGAAATCC GGCCACAGCT ACACAGGCAC GGTGTCCAAA GTCTATGACG 251 GCGATACCCT TCACGTCATC GACGGCGACG GCGCGAAACA TAAAATTCGG 301 ATGGCGTATA TCGACGCACC GGAGATGAAA CAGGCTTACG GTACACGTTC 351 GCGCGACAAC CTGCGCGCGG CGGCGGAGGG TAGGAAAGTC AGTGTACGTG 401 TGTTTGAAAC CGACCGCTAT CAGCGCGAAG TGGCGCAGGT ATCCGCCGGC 451 AAAACCGATT TGAACCTGAT GCAGGTGCAG GACGGGGCGG CGTGGCATTA 501 TAAAAGTTAT GCTAAAGAAC AGCAGGATAA GGCGGATTTT GCCGACTATG 551 CCGACGCTCA AATTCAGGCG GAAAGGGAAC GCAAAGGATT GTGGAAAGCT 601 AAAAATCCGC AAGCGCCGTG GGCGTACCGC CGGGCAGGCA GGAGCGGCGG 651 GGGCAATAAG GATTGGATGG ATTCCGTGGG CGAATGGTTG GGCATTTGGT 701 AA This corresponds to the amino acid sequence <SEQ ID 2996 ORF 992.ng>: g992.pep 1 MFRRHRHLKΝ MQIKKIMKWL PVALSLLGAL GYTGYDSEAV RTAVAVLDVL 51 GTAGDVGFDA PVRRRASAKS GHSYTGTVSK VYDGDTLHVI DGDGAKHKIR 101 MAYIDAPEMK QAYGTRSRDΝ LRAAAEGRKV SVRVFETDRY QREVAQVSAG 151 KTDLΝLMQVQ DGAAWHYKSY AKEQQDKADF ADYADAQIQA ERERKGLWKA 201 KΝPQAPWAYR RAGRSGGGΝK DWMDSVGEWL GIW* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 2997>: m992.seq 1 ATGTTCAGAC GGCATCGGCA TTTGAAAAAT ATGCAGATTA AAAAAATCAT 51 GAAATGGCTT CCCGTCGCCC TGTCGCTTTT GGGTGCGTTG GGTTATACGG 101 GGTACGGCAG CGAGGCGGTG CGGACGGCGG TTGCCGTACT CGACGTACTC 151 GGCGCGGCAG GGGACGCGGG TTCCGACGCG CCCGCCCGCC GCCGAGCATC 201 GGCGAAATCC GGCCACCGCT ACACAGGCAC GGTGTCCAAA GTCTATGACG 251 GCGACACCCT TCACGTTATC GACGGCGACG GCGCGAAACA CAAAATCCGG 301 ATGGCGTATA TCGACGCGCC GGAGATGAAA CAGGCTTACG GCACGCGTTC 351 GCGCGACAAC CTGCGCGCGG CGGCGGAAGG CAGGAAAGTC AGCGTGCGCG 401 TGTTCGATAC CGACCGCTAC CAGCGCGAAG TGGCGCAGGT TTCTGTCGGC 451 AAAACCGATT TGAACCTGAT GCAGGTGCAG GACGGGGCGG CGTGGCATTA 501 TAAAAGTTAT GCTAAAGAAC AGCAGGATAA GGCGGATTTT GCCGATTATG 551 CCGACGCTCA AATTCAGGCG GAAAGGGAAC GCAAAGGATT GTGGAAAGCT 601 AAAAATCCGC AAGCGCCGTG GGCGTACCGC CGAGCAGGCA GGAGCGGCGG 651 GGGCAATAAG GATTGGATGG ATGCCGTGGG CGAATGGTTG GGCATTTGGT 701 AA This corresponds to the amino acid sequence <SEQ ID 2998; ORF 992>: m992.pep 1 MFRRHRHLKΝ MQIKKIMKWL PVALSLLGAL GYTGYGSEAV RTAVAVLDVL 51 GAAGDAGSDA PARRRASAKS GHRYTGTVSK VYDGDTLHVI DGDGAKHKIR 101 MAYIDAPEMK QAYGTRSRDΝ LRAAAEGRKV SVRVFDTDRY QREVAQVSVG 151 KTDLΝLMQVQ DGAAWHYKSY AKEQQDKADF ADYADAQIQA ERERKGLWKA 201 KΝPQAPWAYR RAGRΞGGGΝK DWMDAVGEWL GIW* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 992 shows 96.1% identity over a 233 aa overlap with a predicted ORF (ORF 992) from N gonorrhoeae m992/g992 96.1% identity in 233 aa overlap 10 20 30 40 50 60 m992.pep MFRRHRHLKNMQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAGDAGSDA I I I II I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I : I II g992 MFRRHRHLKNMQIKKIMKWLPVALSLLGALGYTGYDSEAVRTAVAVLDVLGTAGDVGFDA 10 20 30 40 50 60 70 80 90 100 110 120 m992.pep PARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQAYGTRΞRDN I : II I I I I I I I I I I II I I I I I I I II I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I g992 PVRRRASAKSGHSYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQAYGTRSRDN 70 80 90 100 110 120 130 140 150 160 170 180 m992.pep LRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSYAKEQQDKADF I I II I I I I I I I I I I I : I I I I I I I I I I I M I I I I I I I I I I I I I I I I I I I I I I I I II I I I II g992 LRAAAEGRKVSVRVFETDRYQREVAQVSAGKTDLNLMQVQDGAAWHYKSYAKEQQDKADF 130 140 150 160 170 180 190 200 210 220 230 m992.pep ADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGEWLGIWX II I II II I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I M I I I I I I I I I g992 ADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDSVGEWLGIWX 190 200 210 220 230 The following partial DNA sequence was identified in N. meningitidis <SEQ ID 2999>: a992.seq 1 ATGTTCAGAC GGCATCGGCA TTTGAAAAAT ATGCAGATTA AAAAAATCAT 51 GAAATGGCTT CCCGTCGCCT TGTCGCTTTT GGGTGCGTTG GGTTATACGG 101 GGTACGGCAG CGAGGCGGTG CGGACGGCGG TTGCCGTACT CGACGTACTC 151 GGCGCGGCAG GGGACGCGGG TTCCGACGCG CCCGCCCGCC GCCGAGCATC 201 GGCGAAATCC GGCCACCGCT ACACAGGCAC GGTGTCCAAA GTCTATGACG 251 GCGACACCCT TCACGTTATC GACGGCGACG GCGCGAAACA CAAAATCCGG 301 ATGGCGTATA TCGACGCGCC GGAGATGAAA CAGGCTTACG GCACGCGTTC 351 GCGCGACAAC CTGCGCGCGG CGGCGGAAGG CAGGAAAGTC AGCGTCCGCG 401 TGTTCGACAC CGACCGCTAC CAGCGCGAAG TGGCGCAGGT TTCTGTCGGC 451 AAAACCGATT TGAACCTGAT GCAGGTGCAG GACGGGGCGG CGTGGCATTA 501 TAAAAGTTAT GCTAAAGAAC AGCAGGATAA GGCGGATTTT GCCGATTATG 551 CCGACGCTCA AATTCAGGCG GAAAGGGAAC GCAAAGGATT GTGGAAAGCT 601 AAAAATCCGC AAGCGCCGTG GGCGTACCGC CGGGCAGGCA GGAGCGGCGG 651 GGGCAATAAG GATTGGATGG ATGCCGTGGG CGAATGGTTG GGCATTTGGT 701 AA This corresponds to the amino acid sequence <SEQ ID 3000; ORF 992. a>: a992.pep 1 MFRRHRHLKΝ MQIKKIMKWL PVALSLLGAL GYTGYGSEAV RTAVAVLDVL 51 GAAGDAGSDA PARRRASAKS GHRYTGTVSK VYDGDTLHVI DGDGAKHKIR 101 MAYIDAPEMK QAYGTRSRDΝ LRAAAEGRKV ΞVRVFDTDRY QREVAQVSVG 151 KTDLΝLMQVQ DGAAWHYKSY AKEQQDKADF ADYADAQIQA ERERKGLWKA 201 KΝPQAPWAYR RAGRSGGGΝK DWMDAVGEWL GIW* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 992 shows 100.0% identity over a 233 aa overlap with a predicted ORF (ORF 992) from N meningitidis a992/m992 100.0% identity in 233 aa overlap 10 20 30 40 50 60 a992. pep MFRRHRHLKΝMQIKKIMKWLPVALΞLLGALGYTGYGSEAVRTAVAVLDVLGAAGDAGSDA I I I I I I I I I I I II I I I I I I I II I I I I II I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I m992 MFRRHRHLKΝMQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAGDAGSDA 10 20 30 40 50 60 70 80 90 100 110 120 a992.pep PARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQAYGTRSRDΝ I I I I I II I I I I I I I I I I II I I I I I I I II I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I m992 PARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQAYGTRSRDΝ 70 80 90 100 110 120 130 140 150 160 170 180 a992. pep LRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSYAKEQQDKADF I I II II I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I II I I II I m992 LRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSYAKEQQDKADF 130 140 150 ' 160 170 180 190 200 210 220 230 a992.pep ADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGEWLGIWX I II I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I m992 ADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGEWLGIWX 190 200 210 220 230 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 3001>: g993.seq 1 CTGAAAGTCG TATTGGGCAG TTTTCAAGGC CCTTTGGATC TGCTGCTCTA 51 CCTTATCCGC AAGCAGAACA TCGATGTTCT' CGATATTCCG ATGGTGGAAA 101 TTACCGGGCA GTATCTGCAC TATATTGCCC AAATGGAAGC CTATCAGTTT 151 GATTTGGCGG CGGAATATCT TTTGATGGCG GCAATGCTGA TTGAAATCAA 201 ATCGCGCCTG CTGCTGCCGC GTACCGAAGC CGTCGAAGAC GAAGAGGCCG 251 ACCCGCGTGC CGAGTTGGTG CGCCGTCTGC TTGCCTACGA GCAAATGAAA 301 CTGGCGGCGC AGGGTTTGGA CGCGCTGCCG CGTGCGGGAC GGGATTTCGC 351 GTGGGCTTAC CTGCCGCTGG AAATTGCAGC CGAGACGAAG CTGCCCGAGG 401 TTTACATCGC CGATTTGATG CAGGCATGGT TGGGCATTCT TTCTCGGGCA 451 AAACATACGC GCAGCCACGA AGTAATCCAA GAAACCCTTT CCGTGCGCGC 501 GCAAATGACG GCAATCCTGC GCCGTTTGAA CGAACACGGG ATATGCAGGT 551 TTCACGCCCT GTTCAATCCC GAACAGGGCG CGGCTTACGT GATCGTCAAC 601 TTCATCGCCC TGTTGGAGCT TGCCAAAGAA GGATTGGTCG GAATCGTACA 651 GGAAGACGGT TTCGGAGAAA TCCGAATCAG CCTCAATCAT GAGGGGGCGC 701 ATTCAGACGG CATTTTCGGC ACACGGGGCG GGCGCGATGT GTTCTAA This corresponds to the amino acid sequence <SEQ ID 3002 ORF 993. ng>: g993.pep 1 KWLGSFQG PLDLLLYLIR KQΝIDVLDIP MVEITGQYLH YIAQMEAYQF 51 DLAAEYLLMA AMLIEIKSRL LLPRTEAVED EEADPRAELV RRLLAYEQMK 101 LAAQGLDALP RAGRDFAWAY LPLEIAAETK LPEVYIADLM QAWLGILSRA 151 KHTRSHEVIQ ETLSVRAQMT AILRRLΝEHG ICRFHALFΝP EQGAAYVIVΝ 201 FIALLELAKE GLVGIVQEDG FGEIRIΞLΝH EGAHSDGIFG TRGGRDVF* The following partial DΝA sequence was identified in N. meningitidis <SEQ ID 3003>: m993.seq 1 TTGAAAGTCG TATTGGGCAG CTTCCAAGGC CCTTTGGATC TACTGCTGTA 51 TCTGATCCGC AAACAGAATA TCGACGTACT GGATATTCCG ATGGTGAAGA 101 TTACCGAGCA GTATCTGCAC TACATCGCCC AAATAGAAAC CTATCAGTTT 151 GATTTGGCGG CGGAATATCT TTTGATGGCA GCAATGCTGA TTGAAATCAA .201 ATCGCGCCTG CTGCTGCCGC GTACCGAAAC CGTCGAAGAC GAAGAAGCCG 251 ACCCGCGTGC CGAGTTGGTG CGCCGCCTGC TGGCTTACGA ACAGATGAAG 301 CTGGCGGCGC AGGGTTTGGA CGCGCTGCCC CGAGCCGGAC GGGATTTCGC 351 GTGGGCTTAC CTGCCGCTGG AAATTGCCGT CGAAGCCAAG CTGCCCGAAG 401 TCTATATTAC CGACTTGACG CAAGCGTGGC TGGGTATTTT GTCTCGGGCA 451 AAACACACGC GCAGCCACGA AGTAATCAAA GAAACCATCT CCGTGCGCGC 501 GCAAATGACG GCAATCCTGC GCCGTTTGAA CGGACACGGA ATATGCAGGT 551 TTCACGACCT GTTCAATCCC AAACAGGGCG CGGCTTACGT GGTCGTCAAC 601 TTCATCGCAC TGTTGGAGCT TGCCAAAGAA GGATTGGTCA GAATCGTGCA 651 GGAAGACGGT TTCGGAGAAA TCCGAATCAG CCTCAATCAT GAGGGGGCGC 701 ATTCAGACGG CATTTCCGGC ACACGAGGCG GGCGCGATGT GTTCTAA This corresponds to the amino acid sequence <SEQ ID 3004; ORF 993>: m993.pep 1 LKWLGSFQG PLDLLLYLIR KQΝIDVLDIP MVKITEQYLH YIAQIETYQF 51 DLAAEYLLMA AMLIEIKSRL LLPRTETVED EEADPRAELV RRLLAYEQMK 101 LAAQGLDALP RAGRDFAWAY LPLEIAVEAK LPEVYITDLT QAWLGILSRA 151 KHTRSHEVIK ETISVRAQMT AILRRLΝGHG ICRFHDLFΝP KQGAAYVWΝ 201 FIALLELAKE GLVRIVQEDG FGEIRISLΝH EGAHΞDGISG TRGGRDVF* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 993 shows 93.1% identity over a 248 aa overlap with a predicted ORF (ORF 993) from N gonorrhoeae m993/g993 93 . 1 % identity in 248 aa overlap 10 20 30 40 50 60 m993 . pep LKVVLGSFQGPLDLLLYLIRKQNIDVLDI PMVKITEQYLHYIAQIETYQFDLAAEYLLMA I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I I I : M I I I I I I I I I I I I I g993 LKWLGSFQGPLDLLLYLIRKQNIDVLDI PMVEITGQYLHYIAQMEAYQFDLAAEYLLMA 10 20 30 40 50 60 70 80 90 100 110 120 m993. pep AMLIEIKSRLLLPRTETVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAY II I I I I I II I I I I I I I : I I I I I I I I I I I II I I I I I I I II I I I I I I I I I I I I I I I I I I I I I g993 AMLIEIKSRLLLPRTEAVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAY 70 80 90 100 110 120 130 140 150 160 170 180 m993.pep LPLEIAVEAKLPEVYITDLTQAWLGILSRAKHTRΞHEVIKETISVRAQMTAILRRLNGHG I II I I I : M I I I I II I : I I I I I I I II I I I I I I I I I I I I : I I : I I I I I I I I I I I I I I II g993 LPLEIAAETKLPEVYIADLMQAWLGILSRAKHTRSHEVIQETLSVRAQMTAILRRLNEHG 130 140 150 160 170 180 190 200 210 220 230 240 m993 . pep ICRFHDLFNPKQGAAYWVNFIALLELAKEGLVRIVQEDGFGEIRIΞLNHEGAHSDGI SG I I I I I I I I I : I I I I I I : | I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I g993 ICRFHALFNPEQGAAYVIVNFIALLELAKEGLVGIVQEDGFGEIRISLNHEGAHSDGIFG 190 200 210 220 230 240 249 m993. ep TRGGRDVFX g993 TRGGRDVFX The following partial DNA sequence was identified in N meningitidis <SEQ ID 3005>: a993.seq 1 CTGAAAGTCG TATTGAGCAG TTTTCAAGGC CCTTTGGATC TGCTGCTCTA 51 CCTTATCCGC AAGCAGAACA TCGATGTTCT CGATATTCCG ATGGTGAAGA 101 TTACCGAACA GTATCTGCAC TACATCGCCC AAATAGAAAC CTATCAGTTT 151 GATTTGGCGG CGGAATATCT TTTGATGGCA GCAATGCTGA TTGAAATCAA 201 ATCGCGCCTG CTGCTGCCGC GTACCGAAAC CGTCGAAGAC GAAGAAGCCG 251 ACCCGCGTGC CGAGTTGGTG CGCCGCCTGC TGGCTTACGA GCAGATGAAG 301 CTGGCGGCAC AAGGGTTGGA TGCGCTTCCT CGTGCGGGCC GGGATTTCGC 351 ATGGGCATAC CTGCCACTGG AAATTGCCGT CGAAGCCAAG CTGCCCGAAG 401 TCTATATTAC CGACTTGACG CAGGCGTGGC TGAGTATTTT GTCTCGGGCA 451 AAACATACGC GCAGCCACGA AGTTATCAAA GAAACCATCT CCGTGCGCGC 501 GCAAATGACG GCAATCCTGC GCCGTTTGAA CAAACACGGG ATATGCAGGT 551 TTCACGACCT GTTCAATCCC GAACAGGGCG CGGCTTACGT GGTCGTCAAC 601 TTCATCGCAC TGTTGGAGCT TGCCAAAGAA GGTTTGGTCG GAATCGTACA 651 GGAAGTCGGT TTCGGAGAAA TCCGAATCAG CCTCAATCAT GAGGGGGCGC 701 ATTCAGACGG CATTTCCGGC ACACGGGGCG GGCGCGATGT GTTCTAA This corresponds to the amino acid sequence <SEQ ID 3006; ORF 993. a>: a993.pep 1 KVVLSSFQG PLDLLLYLIR KQΝIDVLDIP MVKITEQYLH YIAQIETYQF 51 DLAAEYLLMA AMLIEIKSRL LLPRTETVED EEADPRAELV RRLLAYEQMK 101 LAAQGLDALP RAGRDFAWAY LPLEIAVEAK LPEVYITDLT QAWLSILSRA 151 KHTRSHEVIK ETIΞVRAQMT AILRRLΝKHG ICRFHDLFΝP EQGAAYWVΝ 201 FIALLELAKE GLVGIVQEVG FGEIRISLΝH EGAHSDGISG TRGGRDVF* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 993 shows 97.6% identity over a 248 aa overlap with a predicted ORF (ORF 993) from N meningitidis a993/m993 97.6% identity in 248 aa overlap 10 20 30 40 50 60 a993 . pep LKWLΞSFQGPLDLLLYLIRKQΝIDVLDI PMVKITEQYLHYIAQIETYQFDLAAEYLLMA I I I I I : I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m993 LKWLGSFQGPLDLLLYLIRKQΝIDVLDI PMVKITEQYLHYIAQIETYQFDLAAEYLLMA 10 20 30 40 50 60 70 80 90 100 110 120 a993.pep AMLIEIKSRLLLPRTETVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAY I I I I I II I I I I I I I I I II I I I I I I I II I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m993 AMLIEIKSRLLLPRTETVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAY 70 80 90 100 110 120 130 140 150 160 170 180 a993.pep LPLEIAVEAKLPEVYITDLTQAWLSILSRAKHTRSHEVIKETISVRAQMTAILRRLNKHG I II I I I I I I I II I II I I I I I I I I I : I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I II m993 LPLEIAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHG 130 140 150 160 170 180 190 200 210 220 230 240 a993 . pep ICRFHDLFNPEQGAAYVWNFIALLELAKEGLVGIVQEVGFGEIRISLNHEGAHSDGISG I I I I I I I I I I : I I I I I I I I I I I I I I I I I I I I I I l l l l I I I I I I I I I I I I I I I I I I I I I m993 ICRFHDLFNPKQGAAYWVNFIALLELAKEGLVRIVQEDGFGEIRISLNHEGAHSDGISG 190 200 210 220 230 240 249 a993.pep TRGGRDVFX lllllllll m993 TRGGRDVFX The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 3007>: g996. seq 1 ATGAACAGAA GAACCTTCCT CCTCGGCGCA GGCGCGTTGC TTCTTACCGC 51 CTGCGGCAGA AAATCCGCCC GAACCCACGC CAAAATTCCC GAAGGAAGCA 101 CCGTGCTTGC CTTGGGCGAT TCGCTCACCT TCGGCTACGG AGCAAACCCC 151 GGCGAATCCT ACCCCGCGCA ACTGCAAAAA CTGACGGGTT GGAATATTGT 201 CAACGGCGGC GTATCGGGCG ATACGTCCGC GCAAGCCCTA TCGCGCCTGC 251 CCGCGCTGTT GGCACGCAAA CCCAAGCTTG TGATTGTCGG CATAGGCGGC 301 AACGACTTTC TGCGCAAAGT TCCCGAGGAG CAGACCCGCG CCAATATCGC 351 GAAAATCATC GAAACCGTGC AAAAGGAAAA CATTCCCGCC GTCCTCGTCG 401 GCGTGCCGCA CATCACACTG GGCGCGTTGT TCGGGCATTT GAGCGACCAT 451 CCGCTGTATG AGGATTTGTC CGAGGAATAC GGCATTCCGT TGTTCGGCGG 501 CGCGTGGGCG GAAATTTTGG GCAATAATAA TCTGAAATCC GACCAAATCC 551 ACGCCAACGG CAAAGGCTAT CGGAAATTCG CCGAAAATTT GAATCAATTT 601 TTGAGAAAAC ATGGGTTTAG ATAA This corresponds to the amino acid sequence <SEQ ID 3008 ORF 996.ng>: g996.pep 1 MΝRRTFLLGA GALLLTACGR KSARTHAKIP EGΞTVLALGD SLTFGYGAΝP 51 GEΞYPAQLQK LTGWΝIVΝGG VSGDTΞAQAL SRLPALLARK PKLVIVGIGG 101 ΝDFLRKVPEE QTRAΝIAKII ETVQKEΝIPA VLVGVPHITL GALFGHLSDH 151 PLYEDLSEEY GIPLFGGAWA EILGΝΝΝLKS DQIHAΝGKGY RKFAEΝLΝQF 201 LRKHGFR* The following partial DΝA sequence was identified in N meningitidis <SEQ ID 3009>: m996.seq 1 ATGAACAGAA GAACCTTCCT CCTCGGCGCA GGCGCGTTGC TGCTTACCGC 51 CTGCGGCAGA AAATCCGCCC GAACCCACGC CAAAATTCCC GAAGGAAGCA 101 CCGTACTTGC CTTGGGCGAT TCGCTTACCT TCGGCTACGG CGCAAACCCT 151 GGCGAATCCT ACCCCGCGCA ACTGCAAAAA CTGACGGGTT GGAATATTGT 201 CAACGGCGGC GTATCGGGCG ATACATCTGC CCAAGCCCTG TCGCGCCTGC 251 CCGCGCTGTT GGCACGCAAA CCCAAGCTTG TGATTGTCGG CATAGGCGGC 301 AACGACTTTC TGCGCAAAGT TCCCAAGGAG CAGACCCGCG CCAATATCGC 351 GAAAATCATC GAAACCGTGC AGAAGGAAAA CATCCCCGCC GTCCTCGTCG 401 GCGTGCCGCA CATCACACTG GGTGCGTTGT TCGGGCATTT GAGCGATCAT 451 CCGCTGTATG AGGATTTGTC CGAGGAATAC GGCATTCCGC TGTTCGGCGG 501 CGCGTGGGCG GAAATTTTGG GCGATAATAA TCTGAAATCC GACCAAATCC 551 ACGCCAACGG CAAAGGCTAT CGGAAATTTG CCGAAGATTT GAATCAATTT 601 TTGAGAAAAC AGGGGTTTAG ATAA This corresponds to the amino acid sequence <SEQ ID 3010; ORF 996>: m996.pep 1 MΝRRTFLLGA GALLLTACGR KSARTHAKIP EGSTVLALGD SLTFGYGAΝP 51 GESYPAQLQK LTGWΝIVΝGG VSGDTSAQAL SRLPALLARK PKLVIVGIGG 101 NDFLRKVPKE QTRANIAKII ETVQKENIPA VLVGVPHITL GALFGHLSDH 151 PLYEDLSEEY GIPLFGGAWA EILGDNNLKS DQIHANGKGY RKFAEDLNQF 201 LRKQGFR Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 996 shows 98.1% identity over a 207 aa overlap with a predicted ORF (ORF 996) from N. gonorrhoeae m996/g996 98.1% identity in 207 aa overlap 10 20 30 40 50 60 m996.pep MΝRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLALGDSLTFGYGAΝPGESYPAQLQK I II II I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I II II I I I II I I I I I I I II I I g996 MΝRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLALGDSLTFGYGAΝPGESYPAQLQK 10 20 30 40 50 60 70 80 90 100 110 120 m996.pep LTGWΝIVΝGGVSGDTSAQALSRLPALLARKPKLVIVGIGGΝDFLRKVPKEQTRAΝIAKII II I I I I I II II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I I I I I II I g996 LTGWΝIVΝGGVSGDTSAQALSRLPALLARKPKLVIVGIGGΝDFLRKVPEEQTRAΝIAKII 70 80 90 100 110 120 130 140 150 160 170 180 ιrι996.pep ETVQKEΝIPAVLVGVPHITLGALFGHLSDHPLYEDLSEEYGIPLFGGAWAEILGDΝΝLKS II I I I I I I I I II II I I I I I I I I I I II I I I I I I I II I I I I I I I I I I I I I I I I I I I : I I I II g996 ETVQKEΝIPAVLVGVPHITLGALFGHLΞDHPLYEDLSEEYGIPLFGGAWAEILGΝΝΝLKS 130 140 150 160 170 180 190 200 m996.pep DQIHAΝGKGYRKFAEDLΝQFLRKQGFR I II I I I I I I I I I I I I : I I I I I I Ml I I g996 DQIHAΝGKGYRKFAEΝLΝQFLRKHGFRX 190 200 The following partial DΝA sequence was identified in N meningitidis <SEQ ID 3011>: a996.seq 1 ATGAACAGAA GAACCTTCCT CCTCGGCGCA GGCGCGTTGC TCCTTACCGC 51 CTGCGGCAGA AAATCCGCCC GAACCCACGC CAAAATTCCC GAAGGAAGCA 101 CCGTACTTGC CTTGGGCGAT TCGCTTACCT TCGGCTACGG CGCAAACCCC 151 GGCGAATCCT ACCCCGCGCA ACTGCAAAAA CTGACGGGTT GGAATATTGT 201 CAACGGCGGC GTATCGGGCG ATACATCCGC CCAAGCCCTG TCGCGCCTGC 251 CCGCGCTGTT GGCACGCAAA CCCAAGCTTG TGATTGTCGG CATAGGCGGC 301 AACGACTTTC TGCGCAAAGT TCCCAAGGAG CAGACCCGCG CCAATATCGC 351 GAAAATCATC GAAACCGTGC AGAAGGAAAA CATCCCCGCC GTCCTCGTCG 401 GCGTGCCGCA CATTACCTTG GGCGCGTTGT TCGGGCATTT GAGCGATCAT 451 CCGCTGTATG AGGATTTGTC CGAGGAATAC GGCATTCCGC TGTTCGGCGG 501 CGCGTGGGCG GAAATTTTGG GCGATAATAA TCTGAAATCC GACCAAATCC 551 ACGCCAACGG CAAAGGCTAT CGGAAATTTG CCGAAGATTT GAATCAATTT 601 TTGAGAAAAC AGGGGTTTAG ATAA This corresponds to the amino acid sequence <SEQ ID 3012; ORF 996. a>: a996.pep 1 MΝRRTFLLGA GALLLTACGR KSARTHAKIP EGSTVLALGD SLTFGYGAΝP 51 GESYPAQLQK LTGWΝIVΝGG VSGDTSAQAL SRLPALLARK PKLVIVGIGG 101 NDFLRKVPKE QTRANIAKII ETVQKENIPA VLVGVPHITL GALFGHLSDH 151 PLYEDLSEEY GIPLFGGAWA EILGDNNLKS DQIHANGKGY RKFAEDLNQF 201 LRKQGFR* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 996 shows 100.0% identity over a 207 aa overlap with a predicted ORF (ORF 996) from N meningitidis a996/ 996 100.0% identity in 207 aa overlap 10 20 30 40 50 60 a996.pep MΝRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLALGDSLTFGYGAΝPGESYPAQLQK I I I I I I I I II I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I m996 MNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLALGDSLTFGYGANPGESYPAQLQK 10 20 30 40 50 60 70 80 90 100 110 120 a996.pep LTGWNIVNGGVSGDTΞAQALSRLPALLARKPKLVIVGIGGNDFLRKVPKEQTRANIAKII I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I m996 LTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIVGIGGNDFLRKVPKEQTRANIAKII 70 80 90 100 110 120 130 140 150 160 170 180 a996.pep ETVQKENIPAVLVGVPHITLGALFGHLSDHPLYEDLSEEYGIPLFGGAWAEILGDNNLKS I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I II I I I II I I I I I I I I I I I I I m996 ETVQKENIPAVLVGVPHITLGALFGHLΞDHPLYEDLSEEYGIPLFGGAWAEILGDNNLKS 130 140 150 160 170 180 190 200 a996.pep DQIHANGKGYRKFAEDLNQFLRKQGFRX I I I I I II I I I I I I I I I I I I I I I I I I I I m996 DQIHANGKGYRKFAEDLNQFLRKQGFR 190 200 The following partial DNA sequence was identified in N gonorrhoeae <SEQ ID 3013>: g99 . seq (partial) 1 ATGATGAACA CGCCGCATCC GCGCCCGAAA ATCGCCGTCA TCGGCGCAGG 51 CTGGGCCGGC TTGTCCGCCG CCGTCACCTT GGCGCGGCAC GCCGACGTTA 101 CCCTGTTTGA AGCCGGCCGG CAGGCGGGCG GAAGGGCGCG CACACTGGCC 151 GGAAATACCG ACGGTTTCGG TTTTTTGGAC AACGGGCAGC ACATTTTGCT 201 CGGCGCATAC CGGGGCGTGT TGCGCCTGAT GAAAACCATC GGTTCAGACC 251 CCCGTGCCGC CTTTTTGCGC GTACCGCTGC ACTGGCATAT GCACGGCGGT 301 TTGCAGTTCC GCGCCCTCCC CCTGCCCGCG CCGCTGCATA TTTTGGGCGG 351 CGTGCTGCTT GCCCGGCGCG TACCGTCCGC ATTCAAAGCC AAACTGCTTG 401 CCGATATGTC CGATTTGCAG AAGTCCGCAC GCCTCGGACA GCCCGACACG 451 ACAGTTGCAC AATGGCTGAA ACAGCGGAAC GTGCCGCGTG CCGCCGTAAT 501 GCAGTTTTGG CAGCCCTTGG TCTGGGGCGC GCTCAACACG CCTTTGGAAA 551 CCGCAAGCCT GCGCGTGTTG TGCAACGTTT TGTCCGACGG CGTGCTGACG 601 AAAAAATCCG GCAGCGACTA TCTCCTACCC AAACAGGATT TGGGCGCAAT 651 CGTCGCCGAA CCCGCCTTGG CGGAGCTTCA ACGGCTCGGC GCGGACATCC 701 GCCTCGAAAC GCGCGTATGC CGTCTGAACA CCCTCCCGGA CGGAAAAGTC 751 CTCGTCAACG GCGAAGCCTT CGATGCCGCC ATACTTGCCA CCGCGCCCTA 801 CCACGCCGCC GCGCTCCTGC CCGAAGGCAC GCCCGAACAC GTTCAGACGG 851 CATATCAAAA CCTTCGCTAC CACGCCATCA CCACCGTCTA TCTGCGCTAC 901 GCCGAACCCG TCCGcCTGCc CGCCCCGCTG ACcGGCATtg CCGAcggcAC 951 ggcaCaatgG CTGCTTTgcc cgGGGCAGGC tccggactgc CcccaaAacg 1001 aagTCTCCGC cGTCAttagc GTTTCCGAcc GCGtcggcgC Gtttgcaaac 1051 cga... This corresponds to the amino acid sequence <SEQ ID 3014 ORF 997.ng>: g997.pep (partial) MMΝTPHPRPK IAVIGAGWAG LSAAVTLARH ADVTLFEAGR QAGGRARTLA 51 GΝTDGFGFLD ΝGQHILLGAY RGVLRLMKTI GΞDPRAAFLR VPLHWHMHGG 101 LQFRALPLPA PLHILGGVLL ARRVPSAFKA KLLADMSDLQ KΞARLGQPDT 151 TVAQWLKQRΝ VPRAAVMQFW QPLVWGALΝT PLETASLRVL CΝVLSDGVLT 201 KKSGSDYLLP KQDLGAIVAE PALAELQRLG ADIRLETRVC RLΝTLPDGKV 251 LVΝGEAFDAA ILATAPYHAA ALLPEGTPEH VQTAYQΝLRY HAITTVYLRY 301 AEPVRLPAPL TGIADGTAQW LLCPGQAPDC PQΝEVSAVIS VSDRVGAFAΝ 351 R The following partial DΝA sequence was identified in N meningitidis <SEQ ID 3015>: m997.seq 1 ATGATGAACA CGCCGCATCC GCGCCCGAAA ATCGCCGTCA TCGGCGCAGG 51 CTGGGCAGGA CTGTCCGCCG CCGTCACCTT GGCGCGGCAC GCCGACGTTA 101 CCCTGTTTGA AGCCGGCCGG CAGGCGGGCG GCAGGGCGCG CACACTGGCC 151 GGAAATACCG ACGGTTTCGG TTTTTTGGAC AACGGGCAGC ACATTTTGCT 201 CGGCGCATAC CGGGGCGTGT TGCGCCTGAT GAAAACCATC GGTTCGGATC 251 CCCGTGCCGC CTTTTTGCGC GTACCGCTGC ACTGGCATAT GCACGGCGGT 301 TTGCAGTTCC GCGCCCTCCC CCTGCCCGCG CCGCTGCATA TTTTGGGCGG 351 CGTGCTGCTT GCCCGGCGTG CACCGACTGC ATTCAAAGCC AAACTGCTTG 401 CCGATATGTC CGATTTGCAG AAGTCCGCAC GCCTCGGACA GCCCGACACG 451 ACAGTGGCGC AATGGCTGAA ACAGCGGAAC GTGCCGCGTG CCGCCGTGAT 501 GCAGTTTTGG CAGCCCTTGG TTTGGGGCGC GCTCAACACG CCTTTGGAAA 551 CCGCAAGCCT GCGCGTGTTG TGCAACGTTT TGTCCGACGG CGTGCTGACG 601 AAAAAATCCG GCAGCGACTA TCTCCTACCC AAGCAGGATT TGGGCGCAAT 651 CGTCGCCGAA CCCGCCTTGG CGGATCTTCA ACGGCTCGGC GCGGACATCC 701 GCCTCGAAAC GCGCGTATGC CGTCTGAACA CCCTCCCGGA CGGGAAAGTG 751 CTCGTCAACG GCGAAGCTTT CGATGCCGCC GTCCCCGCCA CCGCGCCCTA 801 CCACGCCGCC GCGCTCCTGC CCGAAGGCAC GCCCGAACAC GTTCAGACGG 851 CATATCAAAA CCTTCGCTAC CACGCCATCA CCACCGTCTA TCTGCGCTAC 901 GCCGAACCCG TCCGCCTGCC CGCCCCGCTG ACCGGCCTTG CCGACGGCAC 951 GGTGCAATGG CTGCTTTGCC GGGGCAGGCT CGGACTGCCT GAAAACGAAG 1001 TGTCCGCCGT CATCAGCGTT TCCGACCGCG TCGGCGCGTT TGCAAACCGG 1051 GCGTGGGCGG ACAAAGCCCA CGCCGACCTC AAACGCATCC TTCCGCATTT 1101 GGGCGAACCC GAAGCCGTGC GCGTCATCAC CGAAAAACGC GCCACAACCG 1151 CAGCCGATGC CCCGCCGCCG GACTTGTCGT GGTTGCACCG GCACCGCATC 1201 TTCCCCGCCG GCGACTACCT CCACCCGGAC TACCCCGCCA CGCTCGAAGC 1251 CGCCGTACAA TCAGGTTTCG CGTCGGCGGA AGCCTGCCTG CAAAGCCTGA 1301 GCGATGCCGT CTGA This corresponds to the amino acid sequence <SEQ ID 3016; ORF 997>: m997.pep 1 MMNTPHPRPK IAVIGAGWAG LSAAVTLARH ADVTLFEAGR QAGGRARTLA 51 GNTDGFGFLD NGQHILLGAY RGVLRLMKTI GSDPRAAFLR VPLHWHMHGG 101 LQFRALPLPA PLHILGGVLL ARRAPTAFKA KLLADMSDLQ KSARLGQPDT 151 TVAQWLKQRN VPRAAVMQFW QPLVWGALNT PLETASLRVL CNVLSDGVLT 201 KKΞGSDYLLP KQDLGAIVAE PALADLQRLG ADIRLETRVC RLNTLPDGKV 251 LVNGEAFDAA VPATAPYHAA ALLPEGTPEH VQTAYQNLRY HAITTVYLRY 301 AEPVRLPAPL TGLADGTVQW LLCRGRLGLP ENEVSAVISV SDRVGAFANR 351 AWADKAHADL KRILPHLGEP EAVRVITEKR ATTAADAPPP DLSWLHRHRI 401 FPAGDYLHPD YPATLEAAVQ SGFAΞAEACL QSLSDAV* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N gonorrhoeae ORF 997 shows 96.0% identity over a 351 aa overlap with a predicted ORF (ORF 997) from N gonorrhoeae g997/m997 96.0% identity in 351 aa overlap 10 20 30 40 50 60 g997.pep MMΝTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGGRARTLAGΝTDGFGFLD I I I I I I I I I I I I I I I I I I I I I I I I I II I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m997 MMΝTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGGRARTLAGΝTDGFGFLD 10 20 30 40 50 60 70 80 90 100 110 120 g997.pep ΝGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQFRALPLPAPLHILGGVLL I I I I I II I I I I I I II I I I I I I I I I I I I I II I I I I I I I II I I I I I I I I I I I I I I I I I I I I I m997 ΝGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQFRALPLPAPLHILGGVLL 70 80 90 100 110 120 130 140 150 160 170 180 g997. pep ARRVPSAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRΝVPRAAVMQFWQPLVWGALΝT II MM I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m997 ARRAPTAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRΝVPRAAVMQFWQPLVWGALΝT 130 140 150 160 170 180 190 200 210 220 230 240 g997.pep PLETASLRVLCΝVLΞDGVLTKKSGSDYLLPKQDLGAIVAEPALAELQRLGADIRLETRVC I I I I I I I I I I I I I II I I I II I I II I II I I I I I I I II I I I I I I I I : I I I I I I I I I I I I I I I m997 PLETASLRVLCΝVLSDGVLTKKSGSDYLLPKQDLGAIVAEPALADLQRLGADIRLETRVC 190 200 210 220 230 240 250 260 270 280 290 300 g997.pep RLΝTLPDGKVLVΝGEAFDAAILATAPYHAAALLPEGTPEHVQTAYQΝLRYHAITTVYLRY I I I I I I I I I I I I I II I I I I M I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I M I m997 RLΝTLPDGKVLVΝGEAFDAAVPATAPYHAAALLPEGTPEHVQTAYQΝLRYHAITTVYLRY 250 260 270 280 290 300 310 320 330 340 350 g997.pep AEPVRLPAPLTGIADGTAQWLLCPGQAPDCPQΝEVSAVISVSDRVGAFAΝR M I I II I II I I Ml II Ml I I I I M M I I I I I II I I I I I I I I I I I I m997 AEPVRLPAPLTGLADGTV Q WLLCRGRL-GLPEΝEVSAVISVSDRVGAFAΝRAWADKAHAD 310 320 330 340 350 The following partial DNA sequence was identified in N meningitidis <SEQ ID 3017>: a997.seq 1 ATGATGAACA CGCCGCATCC GCGCCCGAAA ATCGCCGTCA TCGGCGCAGG 51 CTGGGCCGGC TTGTCCGCCG CCGTTACCTT GGCGCGGCAC GCCGACGTTA 101 CCCTGTTTGA AGCCGGCCGG CAGGCGGGCG GCAGGGCGCG CGCACTGGCC 151 GGAAATACCG ACGGTTTCGG TTTTTTGGAC AACGGGCAGC ATATTTTACT 201 CGGCGCATAC CGGGGCGTGT TGCGCCTGAT GAAAACCATC GGTTCAGACC 251 CCCATGCCGC CTTTTTGCGC GTACCGCTGC ACTGGCATAT GCACGGCGGT 301 TTGCAGTTCC GCGCCCTCCC CCTGCCCGCG CCCCTGCATA TTTTGGGCGG 351 CGTGCTGCTT GCCCGGCGCG TACCGTCCGC ATTCAAAGCC AAACTGCTTG 401 CCGATATGTC CGATTTGCAG AAGTCCGCAC GCCTCGGACA GCCCGACACG 451 ACAGTGGCGC AATGGCTGAA ACAGCGGAAC GTGCCGCGTG CCGCCGTAAT 501 GCAGTTTTGG CAGCCCTTGG TTTGGGGCGC GCTCAACACG CCTTTGGAAA 551 CCGCAAGCCT GCGCGTGTTG TGCAACGTTT TGTCCGACGG CGTGCTGACG 601 AAAAAATCCG GCAGCGACTA TCTCCTACCC AAACAGGATT TGGGCGCAAT 651 CGTCGCCGAA CCCGCCTTGG CGGAGCTTCA ACGGCTCGGC GCGGACATCC 701 GCCTCGAAAC GCGCATATGC CGTCTGAACA CCCTCCCGGA CGGGAAAGTG 751 CTCGTCAACG GCGAACCTTT CGATGCCGCC GTCCCCGCCA CCGCGCCCTA 801 CCACGCCGCC GCGCTCCTGC CCGAAGGCAC GCCCGAACAC GTTCAGACGG 851 CATATCAAAA CCTTCGCTAT CACGCCATCA CCACCGTCTA TCTGCGCTAT 901 GCCGAACCCG TCCGCTTGCC TGCCCCGCTG ACCGGACTTG CCGACGGCAC 951 GGTGCAATGG CTGCTTTGCC GGGGCAGGCT CGGACTGCCT GAAAACGAAG 1001 TGTCCGCCGT CATCAGCGTT TCCGACCGCG TCGGCGCGTT TGCAAACCGG 1051 GCGTGGGCGG ACAAAGTTCA CGCCGACCTC AAACGCATCC TTCCGCATTT 1101 GGGCGAACCC GAAGCCGTGC GCGTCATCAC CGAAAAACGC GCCACAACCG 1151 CAGCCGATGC CCCGCCGCCG GATTTGTCGT GGTTGCACCG GCACCGCATC 1201 TTCCCCGCCG GCGACTACCT CCACCCAGAC TACCCCGCCA CGCTCGAAGC 1251 CGCCGTACAA TCAGGTTTCG CGTCGGCGGA AGCCTGCCTG CAAAGCCTGA 1301 GCGATGCCGT CTGA This corresponds to the amino acid sequence <SEQ ID 3018; ORF 997. a>: a997.pep 1 MMNTPHPRPK IAVIGAGWAG LSAAVTLARH ADVTLFEAGR QAGGRARALA 51 GNTDGFGFLD NGQHILLGAY RGVLRLMKTI GSDPHAAFLR VPLHWHMHGG 101 LQFRALPLPA PLHILGGVLL ARRVPΞAFKA KLLADMSDLQ KSARLGQPDT 151 TVAQWLKQRN VPRAAVMQFW QPLVWGALNT PLETASLRVL CNVLSDGVLT 201 KKSGSDYLLP KQDLGAIVAE PALAELQRLG ADIRLETRIC RLNTLPDGKV 251 LVNGEPFDAA VPATAPYHAA ALLPEGTPEH VQTAYQNLRY HAITTVYLRY 301 AEPVRLPAPL TGLADGTVQW LLCRGRLGLP ENEVSAVISV SDRVGAFANR 351 AWADKVHADL KRILPHLGEP EAVRVITEKR ATTAADAPPP DLSWLHRHRI 401 FPAGDYLHPD YPATLEAAVQ SGFASAEACL QSLSDAV* Computer analysis of this amino acid sequence gave the following results: Homology with a predicted ORF from N meningitidis ORF 997 shows 98.2% identity over a 437 aa overlap with a predicted ORF (ORF 997) from N meningitidis a997/ 997 98.2% identity in 437 aa overlap 10 20 30 40 50 60 a997. pep MMΝTPHPRPKIAVIGAGWAGLΞAAVTLARHADVTLFEAGRQAGGRARALAGΝTDGFGFLD I I II I I I I I I I I I I I I I II I I I I II I I I I I I I I I I I I I II I I I I I I Ml I I I I I I I I I I I m997 MMΝTPHPRPKIAVIGAGWAGLΞAAVTLARHADVTLFEAGRQAGGRARTLAGΝTDGFGFLD 10 20 30 40 50 60 70 80 90 100 110 120 a997.pep ΝGQHILLGAYRGVLRLMKTIGSDPHAAFLRVPLHWHMHGGLQFRALPLPAPLHILGGVLL I I I I I I I I I I I I I I II II I II I I M I II I I I I I I I I I I II I I I I I I I I I I I I I I I II I II m997 ΝGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQFRALPLPAPLHILGGVLL 70 80 90 100 110 120 130 140 150 160 170 180 a997.pep ARRVPΞAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRΝVPRAAVMQFWQPLVWGALΝT I I MM I I I I I I I I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I m997 ARRAPTAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRΝVPRAAVMQFWQPLVWGALΝT 130 140 150 160 170 180 190 200 210 220 230 240 a997.pep PLETASLRVLCΝVLSDGVLTKKSGSDYLLPKQDLGAIVAEPALAELQRLGADIRLETRIC II I I I I I I II I I I I I I I I I I I I I I I I II I II I I I I I I I II I II IM I I I I I I I I I I I IM m997 PLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIVAEPALADLQRLGADIRLETRVC 190 200 210 220 230 240 250 260 270 280 290 300 a997.pep RLNTLPDGKVLVNGEPFDAAVPATAPYHAAALLPEGTPEHVQTAYQNLRYHAITTVYLRY I I I II I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I I I m997 RLNTLPDGKVLVNGEAFDAAVPATAP YHAAALLPEGTPEHVQTAYQNLRYHAI TTVYLRY 250 260 270 280 290 300 310 320 330 340 350 360 a997 . pep AEPVRLPAPLTGLADGTVQWLLCRGRLGLPENEVSAVI SVSDRVGAFANRAWADKVHADL I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I : I I I I m997 AEPVRLPAPLTGLADGTVQWLLCRGRLGLPENEVSAVISVSDRVGAFANRAWADKAHADL 310 320 330 340 350 360 370 380 390 400 410 420 a997.pep KRILPHLGEPEAVRVITEKRATTAADAPPPDLSWLHRHRIFPAGDYLHPDYPATLEAAVQ I I I I I I I I I I I I I II I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I I II I I m997 KRILPHLGEPEAVRVITEKRATTAADAPPPDLSWLHRHRIFPAGDYLHPDYPATLEAAVQ 370 380 390 400 410 420 430 a997.pep SGFASAEACLQSLSDAVX I II I I I I I I I I I I I I I I I m997 SGFASAEACLQSLSDAVX 430 g999.seq Not found yet g999.pep Not found yet The following partial DNA sequence was identified in N meningitidis <SEQ ID 3019>: m999. seq 1 ATGAATATGA AAAAATTGAT TTCCGCAATT TGTGTTTCAA TTGTTTTATC 51 AGCCTGCAAC CAACAATCAA AAACGGCACA AGCCGAAGAA CCTGTCCAAA 101 GTATCCAGGC TGCTGATTGT ACCGCCCCAA TGGACATCAC AGTTGAACAA 151 TATCTCATCA ATTTGGAGCA AGCATTTAAA ACTCAGAACG TCTCAACAAA 201 AATCCATAAT AAAAATATTG TCAAGACCGA TTGTGGTTAT GACCTTACTT 251 TGGTAATGGA TTTTGGGGCG ATTGCGCTCA AACTGGACGA GCAGCAAAAA 301 ATTAGAGCTA TCTCAGTAGG CTACATTTTA AAAACCGACG GAGAGAAAGG 351 ACAAAATCTA GTCAATAATG CCATAAATGG ATTACACAGT ATTCAGGCAG 401 TTCTGTCTTT AACTACCACA GACAAATTGG GCGAATCGGA AGCAGGAAAA 451 CAACTTTTTA CAGCTTTAAC CGAAGTCGTC AAAGAATCCA ATCAGACAGG 501 AGCAACAGCG CAAAAAGACG TTCCGGCAGA TGGTATTTTA TATAGCGTTG 551 TTTTTGAAAA AGAAACAAAC ACCATTGCAA TAATCGGCAG AAAACAACCC 601 TAA This corresponds to the amino acid sequence <SEQ ID 3020; ORF 999>: m999.pep 1 MΝMKKLIΞAI CVSIVLSACΝ QQΞKTAQAEE PVQSIQAADC TAPMDITVEQ 51 YLIΝLEQAFK TQΝVSTKIHΝ KΝIVKTDCGY DLTLVMDFGA IALKLDEQQK 101 IRAISVGYIL KTDGEKGQΝL VΝΝAIΝGLHS IQAVLSLTTT DKLGESEAGK 151 QLFTALTEW KEΞΝQTGATA QKDVPADGIL YΞWFEKETΝ TIAIIGRKQP a999.seq Not found yet a999.pep Not found yet The foregoing examples are intended to illustrate but not to limit the invention.

Claims: CLAIMS 1. A protein comprising a fragment of an amino acid sequence from SEQ ID 2790 wherein said fragment comprises at least 7 amino acids from said sequence. 2. A protein comprising an amino acid sequence selected from the group consisting of even numbered SEQ IDs from SEQ ID number 2 through SEQ ID number 3020. 3. A protein having 50% or greater homology to a protein according to claim 1. 4. A protein comprising a fragment of an amino acid sequence selected from the group consisting of even numbered SEQ IDs from SEQ ID number 2 through SEQ ID number 3020, wherein said fragment comprises 7 or more consecutive amino acids from said sequence. 5. An antibody which binds to a protein according to any one of claims 1 to 3. 6. A nucleic acid molecule which encodes a protein according to any one of claims 1 to 3. 7. A nucleic acid molecule according to claim 5, comprising a nucleotide sequence selected from the group consisting of odd numbered SEQ IDs from SEQ ID number 1 through SEQ ID number 3019. 8. A nucleic acid molecule comprising a fragment of a nucleotide sequence selected from the group consisting of odd numbered SEQ IDs from SEQ ID number 1 through SEQ ID number 3019, wherein said fragment comprises 10 or more consecutive nucleotides from said sequence. 9. A nucleic acid molecule comprising a nucleotide sequence complementary to a nucleic acid molecule according to claim 5. 10. A nucleic acid molecule comprising a nucleotide sequence complementary to a nucleic acid molecule according to claim 6. 11. A nucleic acid molecule comprising a nucleotide sequence complementary to a nucleic acid molecule according to claim 7. 12. A composition comprising a protein, a nucleic acid molecule, or an antibody according to any preceding claim. 13. A composition according to claim 11 being a vaccine composition or a diagnostic composition. 14. A composition according to claim 11 for use as a pharmaceutical. 15. The use of a composition according to claim 11 in the manufacture of a medicament for the treatment or prevention of infection due to Neisserial bacteria. 16. A composition comprising a protein of claim 1 wherein said composition is immunogenic. 17. A composition comprising a protein of claim 2 wherein said composition is immunogenic. 18. A composition comprising a protein of claim 3 wherein said composition is immunogenic.

Inventors: FRASER CLAIRE, GALEOTTI CESIRA, GRANDI GUIDO, HICKEY ERIN, MASIGNANI VEGA, MORA MARIROSA, PETERSEN JEREMY, PIZZA MARIAGRATIA, RAPPUOLI RINO, RATTI GIULIO, SCALATO ENZO, SCARSELLI MARIA, TETTELIN HERVE, VENTER J CRAIG, FRASER, CLAIRE, GALEOTTI, CESIRA, GRANDI, GUIDO, HICKEY, ERIN, MASIGNANI, VEGA, MORA, MARIROSA, PETERSEN, JEREMY, PIZZA, MARIAGRATIA, RAPPUOLI, RINO, RATTI, GIULIO, SCALATO, ENZO, SCARSELLI, MARIA, TETTELIN, HERVE, VENTER, J., CRAIG
Applicants: CHIRON CORP, FRASER CLAIRE, GALEOTTI CESIRA, GRANDI GUIDO, HICKEY ERIN, INST GENOMIC RESEARCH, MASIGNANI VEGA, MORA MARIROSA, PETERSEN JEREMY, PIZZA MARIAGRATIA, RAPPUOLI RINO, RATTI GIULIO, SCALATO ENZO, SCARSELLI MARIA, TETTELIN HERVE, VENTER J CRAIG, CHIRON CORPORATION, FRASER, CLAIRE, GALEOTTI, CESIRA, GRANDI, GUIDO, HICKEY, ERIN, MASIGNANI, VEGA, MORA, MARIROSA, PETERSEN, JEREMY, PIZZA, MARIAGRATIA, RAPPUOLI, RINO, RATTI, GIULIO, SCALATO, ENZO, SCARSELLI, MARIA, TETTELIN, HERVE, THE INSTITUTE FOR GENOMIC RESEARCH, VENTER, J., CRAIG
Application year: 1999
Main classification: C12N15
IPC codes: A61K38, C07K14, C12N15