REACTIVE NANOPARTICLES AS DESTRUCTIVE ADSORBENTS FOR BIOLOGICAL AND CHEMICAL CONTAMINATION

Abstract: A method is provided for destroying a target component, such as hydrocarbons, halogenated hydrocarbons, diethyl-4-nitrophenylphosphate (paraxon), bateria such as Bacillus Cereus, Bacillus Globigii, Chlamydia and/or Rickettsiae, fungi and viruses, by contacting the target component with an adsorbent such as MgO, CaO, TiO2, ZrO2, FeO, V2O5, V2O3, Mn2O3, Fe2O3, NiO, CuO, Al2O3, ZnO, and mixtures thereof wherein the adsorbent contains either reactive atoms selected from the group consisting of halogens and alkali metals stabilized on the surfaces of the adsorbent or ozone and wherein the contacting is conducted at a temperature of -40 to 600 DEG C for a time period of at least about 4 seconds. The adsorbent may be in the form of solids having a size of about 3 to 20 nanometers or in the form of pellets having a size of at least 1 millimeter.

Description: REACTIVE NANOPARTICLES AS DESTRUCTIVE ADSORBENTS FOR BIOLOGICAL AND CHEMICAL CONTAMINATION BACKGROUND OF THE INVENTION Field of the Invention The present invention is broadly concerned with compositions and methods for sorbing and/or destroying dangerous substances such as chemical and biological warfare agents. The methods of the invention are carried out by simply contacting the target substance with particulate metal oxide compositions. These compositions can be unmodified, or alternately, the particulate metal oxides can be coated with a second metal oxide, have reactive atoms or mixtures of reactive atoms stabilized on their surfaces, or have species adsorbed on their surfaces. In another embodiment, the particulate metal oxides (unmodified or modified) can be pressed to form pellets which possess the same destructive abilities as the metal oxides in powder form. Methods in accordance with the invention require the use of minimal liquids, thus resulting in very little effluent. Furthermore, the particulate metal oxide compositions utilized in the methods of the invention are not harmful to equipment or to humans and can easily be used directly at the site of contamination. Description of the Prior Art The threat of biological and chemical warfare has grown considerably in recent times. Numerous countries are capable of developing deadly biological and chemical weapons. Some potent biological agents include the following: bacteria such as Bacillus anthracis (anthrax) and Yersinia pestis (plague); viruses such as variola virus (small pox) and flavi viruses (hemmorhagic fevers); and toxins such as botulinum toxins and saxitoxin. Some potent chemical agents include: blister or vesicant agents such as mustard agents; nerve agents such as methylphosphonothioc acid (VX); lung damaging or choking agents such as phosgene (CG); cyanogen agents such as hydrogen cyanide; incapacitants such as 3-quinuclidinyl benzilate; riot control agents such as CS (orthochlorobenzylidene malononitrile); smokes such as zinc chloride smokes; and some herbicides such as 2,4-D (2-4-dichlorophenoxyacetic acid). All of the above agents, as well as numerous other biological and chemical agents, pose a significant risk to private citizens as well as to military personnel. For example, vesicant agents burn and blister the skin or any other part of the body they contact, including eyes, mucus membranes, lungs, and skin. Nerve agents are particularly toxic and are generally colorless, odorless, and readily absorbable through the lungs, eyes, skin, and intestinal track. Even a brief exposure can be fatal and death can occur in as quickly as 1 to 10 minutes. Biological agents such as anthrax are easily disseminated as aerosols and thus have the ability to inflict a large number of casualties over a wide area with minimal logistical requirements. Many biological agents are highly stable and thus can persist for long periods of time in soil or food. There are currently two general types of decontamination methods for biological agents: chemical disinfection and physical decontamination. Chemical disinfectants, such as hypochlorite solutions, are useful but are corrosive to most metals and fabrics, as well as to human skin. Physical decontamination, on the other hand, usually involves dry heat up to 160°C for 2 hours, or steam or super-heated steam for about 20 minutes. Sometimes UV light can be used effectively, but it is difficult to develop and standardize for practical use. These methods have many drawbacks. The use of chemical disinfectants can be harmful to personnel and equipment due to the corrosiveness and toxicity of the disinfectants. Furthermore, chemical disinfectants result in large quantities of effluent which must be disposed of in an environmentally sound manner. Physical decontamination methods are lacking because they require large expenditures of energy. Both chemical and physical methods are difficult to use directly at the contaminated site due to bulky equipment and/or large quantities of liquids which must be transported to the site. Finally, while a particular decontamination or disinfection method may be suitable for biological decontamination, it is generally not effective against chemical agents. There is a need for decontamination compounds which are effective against a wide variety of both chemical and biological agents, have low energy requirements, are easily transportable, do not harm skin or equipment, and employ small amounts of liquids with minimal or no effluent. SUMMARY OF THE INVENTION The present invention overcomes these problems and provides compositions and methods for sorbing (e.g., adsorption and chemisorption) and destroying biological and chemical agents. To this end, the invention contemplates the use of finely divided nanoscale metal oxide adsorbents. These adsorbents can be used in an unmodified form or can be pelletized, coated with a second metal oxide, or have reactive atoms stabilized on their surfaces. These decontamination reactions can be carried out over a wide range of temperatures and can be conducted at the contaminated site. Furthermore, these adsorbents are not harmful to equipment or to humans. In more detail, the nanoscale adsorbents used in the methods of the invention are formed from metal oxides selected from the group consisting of MgO, CaO, TiO 2 , ZrO 2 , FeO, V 2 O 3 , V 2 O 5 , Mn 2 O 3 , Fe 2 O 3 , NiO, CuO, Al 2 O 3 , ZnO, and mixtures thereof. While conventionally compared powders can be used in the methods of the invention, the preferred powders are prepared by aerogel techniques from Utamapanya et al., Chem. Mater., 3:175-181 (1991), incorporated by reference herein. The adsorbents should have an average crystallite size of up to about 20 nm, preferably from about 3-8 nm, and more preferably 4 nm, and exhibit a Brunauer-Emmett-Teller (BET) multipoint surface area of at least about 15 m 2 /g, preferably at least about 200 m 2 /g, and more preferably about 400 m 2 /g. In terms of pore radius, the preferred adsorbents should have an average pore radius of at least about 45 A, more preferably from about 50-100 A, and most preferably from about 60-75 A. These nanoscale adsorbents can be used alone and in their powder form, or they can be modified. For example, the finely divided particles of the metal oxides can have at least a portion of their surfaces coated with a quantity of a second metal oxide different than the first metal oxide and selected from oxides of metals selected from the group consisting of Ti, V, Fe, Cu, Ni, Co, Mn, Zn and mixtures thereof. In preferred forms, the coated metal oxide particles comprise a first metal oxide selected from the group consisting of MgO and CaO, whereas the second metal oxide is preferably Fe 2 O 3 . For most efficient uses, the particles of the first metal oxide should have the average crystallite sizes and multi-point surface areas set forth above. As is conventional in the art, the term "particles" is used herein interchangeably with the term "crystallite." The second metal oxide should be in the form of an extremely thin layer or coating applied onto the surface of the first metal oxide, thus giving an average overall size for the composite of up to about 21 nm, more preferably from about 5-11 nm, and most preferably about 5 nm. Generally, the first metal oxide should be present in substantial excess relative to the second metal oxide. Thus, the first metal oxide comprises from about 90-99% by weight of the total composite material, and more preferably from about 95-99% by weight. Correspondingly, the second metal oxide should comprise from 1-10% by weight of the total composite, and more preferably from about 1-5% by weight. At least 75% of the surface area of the first metal oxide particles should be covered with the second oxide, and more preferably from about 90-100% of this surface area should be covered. The coated metal oxide particles or crystallites of this embodiment are preferably fabricated by first forming the very finely divided first particulate material using known aerogel techniques. Thereafter, the second material is applied onto the surface of the first metal oxide as an extremely thin layer, e.g., a monolayer having a thickness on the order of less than 1 nm. For example, nanocrystalline MgO can be prepared and then treated with an iron salt such as iron III (acetylacetonate) 3 with the ligands being driven off by heating. In another embodiment, the methods of the invention utilize particulate metal oxides having reactive atoms (different from those atoms making up the metal oxide) stabilized on the surfaces thereof. In more detail, the metal oxide particulates have oxygen ion moieties on their surfaces with reactive atoms interacted or chemisorbed with those surface oxygen ions. The metal oxide particles are, as with the two previously described embodiments, selected from the group consisting of MgO, CaO, TiO 2 , ZrO 2 , FeO, V 2 O 3 , V 2 O 5 , Mn 2 O 3 , Fe 2 O 3 , NiO, CuO, Al 2 O 3 , ZnO, and mixtures thereof. Furthermore, the particles should have the same average crystallite sizes and surface areas described above. Preferably, the reactive atoms utilized in this embodiment are selected from the group consisting of halogens and Group I metals. When halogens are the reactive atoms being stabilized on the surfaces of the particles, the atoms can be atoms of the same halogen (e.g., only chlorine atoms), or the atoms can be mixtures of atoms of different halogens (e.g., both chlorine and bromine atoms on the metal oxide surfaces). When stabilizing a Group I metal atom, the atom loading on the metal oxide should be from about 5-40% by weight, preferably from about 10- 15%) by weight, and more preferably about 12% by weight, based upon the weight of the atom-loaded metal oxide taken as 100%. When stabilizing either a Group I metal atom or a halogen atom, the atom loading on the metal oxide can also be expressed as a concentration of atoms per unit of surface area of the metal oxide, i.e., at least about 2 atoms per square nanometer of metal oxide surface area, preferably from about 3-8 atoms per square nanometer of metal oxide surface area, and more preferably from about 4-5 atoms per square nanometer of metal oxide surface area. The preferred Group I metal is potassium, and the preferred halogens are chlorine and bromine. The surface-stabilized, reactive atom composites are formed by heating a quantity of particulate metal oxide particles to a temperature of at least about 200 °C, preferably at least about 300 °C, and more preferably to a level of from about 450 to about 500°C. Heating the metal oxide particles to these temperatures removes water from the particles so that the final compositions have a surface hydroxyl concentration of less than about 5 hydroxyl groups per square nanometer of metal oxide surface area, and preferably less than about 4 hydroxyl groups per square nanometer of metal oxide surface area. The particles are preferably allowed to cool to room temperature. The particles are then contacted with a source of reactive atoms, e.g., a compound which dissociates into reactive atoms under the proper reaction conditions. The reactive atoms interact with the metal oxide surface oxygen ions, thus stabilizing the atoms on the oxide surface. As used hereinafter, the terms "stabilized" and "stable" mean that, when the metal oxide-atom adducts are heated to a temperature of about 100°C, less than about 10%) of the total weight loss of the adduct is attributable to the reactive atoms desorbing. In another embodiment, the methods of the invention utilize particulate metal oxides having species different than the metal oxide adsorbed on the surfaces thereof. The metal oxide particles are selected from the group consisting of MgO, CaO, TiO 2 , ZrO 2 , FeO, V 2 O 3 , V 2 O 5 , Mn 2 O 3 , Fe 2 O 3 , NiO, CuO, Al 2 O 3 , ZnO, and mixtures thereof. The particles should have the same average crystallite sizes and surface areas described above. Preferably, the adsorbed species are selected from the group consisting of oxides of Group V elements, oxides of Group VI elements, and ozone. Preferred oxides of Group V and VI elements are NO 2 and SO 2 , respectively. When adsorbing a species on the metal oxide surfaces, the species loading on the metal oxide should be from about 1-60%) by weight, preferably from about 5-40% by weight, and more preferably about 15-25% by weight, based upon the weight of the adsorbed species-metal oxide taken as 100%. The species loading can also be expressed as a concentration of species molecules per unit of surface area of metal oxide. Preferably, there are at least about 2 molecules of the species adsorbed per square nanometer of metal oxide and more preferably at least about 5 molecules. The adsorbed-species, metal oxide composites are formed by contacting a quantity of the desired metal oxide (in an air evacuated flask) with the gaseous species. The sample is allowed to react for about 30 minutes, after which time the excess gaseous species is pumped out. In yet another embodiment, the methods of the invention contemplate forming the above metal oxide particles and composites including those particles (i.e., unmodified, finely divided metal oxide particles, finely divided metal oxide particles coated with a second metal oxide, finely divided metal oxide particles having reactive atoms and mixtures of reactive atoms stabilized on the surfaces thereof, and metal oxide particles having species adsorbed on the surfaces thereof) into pellets for use when powdered decontaminants are not feasible. These pellets are formed by pressing a quantity of one of these powdered metal oxide composites at a pressure of from about 50-6,000 psi, more preferably from about 500-5000 psi, and most preferably at about 2,000 psi. While pressures are typically applied to the powder by way of an automatic or hydraulic press, one skilled in the art will appreciate that the pellets can be formed by any pressure-applying means. Furthermore, a binder or filler can be mixed with the adsorbent powder and the pellets can be formed by pressing the mixture by hand. Agglomerating or agglomerated as used hereinafter includes pressing together of the adsorbent powder as well as pressed-together adsorbent powder. Agglomerating also includes the spraying or pressing of the adsorbent powder (either alone or in a mixture) around a core material other than the adsorbent powder. In order to effectively carry out the methods of the invention, the pellets should retain at least about 25% of the multi-point surface area/unit mass of the metal hydroxide or metal oxide (whichever was used to form the pellet) particles prior to pressing together thereof. More preferably, the multi-point surface area/unit mass of the pellets will be at least about 50%, and most preferably at least about 90%), of the multi-point surface area/unit mass of the starting metal oxide or metal hydroxide particles prior to pressing. The pellets should retain at least about 25% of the total pore volume of the metal hydroxide or metal oxide particles prior to pressing thereof, more preferably, at least about 50%, and most preferably at least about 90% thereof. In the most preferred forms, the pellets will retain the above percentages of both the multipoint surface area/unit mass and the total pore volume. The pellets normally have a density of from about .2 to about 2.0 g/cm 3 , more preferably from about .3 to about 1.0 g/cm 3 , and most preferably from about .4 to about .7 g/cm 3 . The minimum surface-to- surface dimension of the pellets (e.g., diameter in the case of spherical or elongated pellet bodies) is at least about 1 mm, more preferably from about 10-20 mm. In carrying out the methods of the invention, one or more of the above described metal oxide particle composites are contacted with the target substance to be sorbed, decontaminated or destroyed under conditions for sorbing, decontaminating or destroying at least a portion of the substance. The methods of the invention provide for destructively adsorbing a wide variety of chemical agents, including agents selected from the group consisting of acids, alcohols, compounds having an atom of P, S, N, Se, or Te, hydrocarbon compounds, and toxic metal compounds. The methods of the invention also provide for biocidally adsorbing a wide variety of biological agents, including bacteria, fungi, viruses, rickettsiae, chlamydia, and toxins. Utilizing the metal oxide particulate composites in accordance with the methods of the invention is particularly useful for biocidally adsorbing biological agents such as bacteria, especially gram positive bacteria like B. globigii and B. cereus. In another embodiment, the methods of the invention provide for the destructive adsorption of hydrocarbon compounds, both chlorinated and non-chlorinated. The contacting step can take place over a wide range of temperatures and pressures. For example, the particulate metal oxide composites can be taken directly to a contaminated site and contacted with the contaminant and/or contaminated surfaces at ambient temperatures and pressures. Alternately, the contacting step can be carried out at a temperature of from about -40-600 °C. If the contacting step is to be carried out under ambient temperatures, preferably the reaction temperature range is from about 10- 200 °C. If the contacting step is to be carried out under high temperature conditions, then preferably the temperature range for the reaction is from about 350-550 °C. If the contacting step is carried out under ambient conditions, the particulate metal oxide composites should be allowed to contact the target substance for at least about 0.5 minutes, preferably from about 1-100 minutes, and more preferably from about 1.5-20 minutes. If the contacting step is carried out under high temperatures conditions, then the particulate metal oxide composites should be allowed to contact the target substance for at least about 4 seconds, preferably for about 5-20 seconds, and more preferably for about 5-10 seconds. If the target substance is a biological agent, the contacting step results in at least about a 90% reduction in the viable units of the biological agent, preferably at least about a 95% reduction, and more preferably at least about a 98% reduction. If the target substance is a chemical agent, the contacting step results in at least about 90% reduction in the concentration of the chemical agent, preferably at least about a 95%> reduction, and more preferably at least about a 99% reduction. Those skilled in the art will appreciate the benefits provided by the methods of the invention. In accordance with the invention, military personnel can utilize the particulate metal oxides and composites thereof to neutralize highly toxic substances such as nerve agents and biological agents. These particles and composites can be utilized in their non-toxic ultrafine powder form to decontaminate areas exposed to these agents, or the highly pelletized composites can be utilized in air purification or water filtration devices. Other countermeasure and protective uses for the metal oxide particles and composites of the particles include personnel ventilation systems and wide-area surface decontamination. Furthermore, the metal oxide composites remain airborne for at least one hour, thus providing effective airborne decontamination of chemical or biological agents. Alternately, the composites can be formulated into a cream or incorporated in or on clothing in order to provide protection to personnel at risk of contacting a dangerous agent. Unlike currently available decontamination methods, the methods of the invention utilize composites that are non-toxic to humans and non-corrosive to equipment, thus permitting the decontaminated equipment to be put back into use rather than discarded. Furthermore, because the composites are easy to disperse and readily transportable, and because little or no water is required to practice the invention, it is relatively simple to destroy the contaminants at the contaminated site. BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is a graph illustrating the particle size distribution and particle concentration for B. globigii without the addition of Cl/AP-MgO powder; Fig. 2 shows the baseline decay curve for B. globigii; Fig. 3 is a graph depicting the Cl/AP-MgO concentration with respect to time when B. globigii was exposed to a low concentration of Cl/AP-MgO; Fig. 4 is a graph illustrating the particle size distribution for the mixture of powder and B. globigii when B. globigii was exposed to a low concentration of Cl/AP- MgO powder; Fig. 5 shows the decay curve for B. globigii when B. globigii was exposed to a low concentration of Cl/AP-MgO; Fig. 6 is a graph depicting the Cl/AP-MgO concentration with respect to time when B. globigii was exposed to a high concentration of Cl/AP-MgO; Fig. 7 is a graph depicting the particle size distribution for a mixture of powder and B. globigii when B. globigii was exposed to a high concentration of Cl/AP-MgO; Fig. 8 is a graph illustrating the decay curve for B. globigii when B. globigii was exposed to a high concentration of Cl/AP-MgO; and Fig.9 is a graph illustrating the destructive adsorption of paraoxon on AP-MgO, I/AP-MgO, and Cl/AP-MgO. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The following examples set forth preferred methods in accordance with the invention. It is to be understood, however, that these examples are provided by way of illustration and nothing therein should be taken as a limitation upon the overall scope of the invention. In these examples, "AP-MgO" and "AP-CaO" refer to the respective aerogel (or autoclave) prepared oxides. "CP-MgO" and "CP-CaO" refer to the respective oxides produced by conventional techniques. EXAMPLE 1 Preparation of MgO Samples 1. AP-MgO Highly divided nanocrystalline Mg(OH) 2 samples were prepared by the autoclave treatment described by Utamapanya et al., Chem. Mater., 3:175-181 (1991), incorporated by reference herein. In this procedure, 10%> by weight magnesium methoxide in methanol solution was prepared and 83 > by weight toluene solvent was added. The solution was then hydrolyzed by addition of 0.15% by weight water dropwise while the solution was stirred and covered with aluminum foil to avoid evaporation. To ensure completion of the reaction, the mixture was stirred overnight. This produced a gel which was treated in an autoclave using a glass lined 600 ml capacity Parr miniature reactor. The gel solution was placed within the reactor and flushed for 10 minutes with nitrogen gas, whereupon the reactor was closed and pressurized to 100 psi using the nitrogen gas. The reactor was then heated up to 265 °C over a 4 hour period at a heating rate of 1 °C/min. The temperature was then allowed to equilibrate at 265 °C for 10 minutes (final reactor pressure was about 800-1000 psi). At this point, the reactor was vented to release the pressure and vent the solvent. Finally, the reactor was flushed with nitrogen gas for 10 minutes. The Mg(OH) 2 particles were then thermally converted to MgO. This was accomplished by heating the Mg(OH) 2 under dynamic vacuum ( 10 "2 Torr) conditions at an ascending temperature rate to a maximum temperature of 500° C which was held for 6 hours resulting in AP-MgO with a BET surface area of 300 - 600 m 2 /g and an average crystallite size of 4nm. Further details about the MgO preparation can be found in PCT Publication WO 95/27679, also incorporated by reference herein. 2. CP-MeO CP-MgO samples were prepared by boiling commercially available MgO (Aldrich Chemical Company) for one hour, followed by microwave drying of the sample. The sample was then dehydrated under vacuum at 500 °C resulting in CP-MgO with a BET surface area of 130-200 m 2 /g and an average crystallite size of 8.8 nm. EXAMPLE 1A Preparation of AP-CaO and CP-CaO AP-CaO was prepared in a manner similar to the preparation of AP-MgO as described in Example 1 with the following exceptions: 8 g. of calcium metal and 230 ml of methanol were allowed to react; and 180 ml of toluene and 1.2 ml of distilled water were added to the 20 g of calcium methoxide obtained. AP-CaO (N 2 dehydrated) was prepared in a similar manner with the exception that the sample was dehydrated by heating to a temperature of 500 °C while passing N 2 gas over the sample. CP-CaO (vacuum dehydrated) was prepared in the same manner as CP-MgO (described in Example 1, Part 2). EXAMPLE IB Preparation of Fe 2 O 3 /MgO Composites Mg(OH) 2 particles were first thermally converted to MgO, followed by deposition of iron oxide to provide the complete composite. The initial thermal conversion of magnesium hydroxide to MgO was carried out by heating the magnesium hydroxide under dynamic vacuum conditions at an ascending temperature rate to a maximum temperature of 500 °C, which was held for 6 hours. Most of the dehydration was found to occur at temperatures between 200 ° C and 320 ° C . IR and x-ray diffraction studies confirm virtually complete conversion of the Mg(OH) 2 to MgO. Iron oxide was deposited on the nanoscale MgO particles by carrying out a direct reaction between activated MgO and iron III (acetylacetonate) 3 , in tetrahydrofuran at room temperature under 1 atmosphere of helium. In a typical preparation, 0.3 grams of Mg(OH) 2 was heated under vacuum ( 10 "3 Torr.) at an ascending temperature rate of 1 °C/min. to 500°C, which was held for 6 hours to assure complete conversion to MgO, followed by cooling to room temperature. The evacuated system was then filled with helium at 1 atm. pressure. Two milliliters of 0.25 M iron III (acetylacetonate) 3 in THF solution (previously prepared under argon by dissolving 4.5 g of iron III (acetylacetonate) 3 in 50 ml of THF) was introduced by a syringe. The amount of iron III (acetylacetonate) 3 solution used provided the MgO surfaces with 1.4 iron III (acetylacetonate) 3 molecules for each surface OH group. The concentration of surface OH groups for the autoclave-prepared MgO was found to be 3.6 OH groups/nm 2 . The reaction mixture was stirred overnight to allow a complete reaction at room temperature. The reacted Fe 2 O 3 /MgO composite was then removed, filtered using regular filter paper, washed with THF to remove any residual iron III (acetylacetonate) 3 , and dried in air for ten minutes. IR spectra of the resultant dry product showed bands for the acetylacetonate species, indicating the existence of some acetylacetonate ligands bound to the surfaces of the MgO. This product was heated again under vacuum (10 "3 Torr.) at 500°C to remove these ligands. EXAMPLE 2 Halogenated Metal Oxides The following procedures were followed to prepare halogenated metal oxides: 1. Chlorinated Metal Oxides In order to prepare Cl/MgO or Cl/CaO, metal oxide samples (weighing from about 0.30 to about 1.0 g each) were placed in a Schlenk tube (340 ml vacuum tight glass tubes). Each sample tube was evacuated at room temperature and an excess of chlorine gas was allowed to enter the tube at a pressure of about 1 atm of chlorine. The amount of chlorine gas was determined to be an excess amount when the inlet gas remained green. The samples became hot to the touch when the chlorine entered the tubes, indicating that a reaction was taking place. The reaction was complete within one to two minutes, but each sample was allowed to stand for approximately 30 minutes before removal from the tube. 2. Brominated Metal Oxides Br/MgO and Br/CaO were prepared in a manner similar to that described under Part 1. An excess of bromine gas was allowed to enter a Schlenk tube which contained from 0.30 to 1.0 g of the particular metal oxide sample at the vapor pressure of bromine at room temperature. The amount of bromine gas was determined to be an excess amount when the inlet gas remained dark red. The reaction was complete within several minutes, but each sample was allowed to stand for approximately 30 minutes before removal from the tube. 3. Iodinated Metal Oxides I/MgO and I/CaO were prepared by placing 1.0 g of the metal oxide in a Schlenk tube along with 1.0 g of iodine. The air was evacuated from the tube, the stopcock was closed, and the mixture was heated to 90-100 °C. The iodine vaporized and deposited onto the oxide particles. The sample was allowed to stand for about 30 minutes before removal from the sample tube. EXAMPLE 3 1. Preparation of Bacillus slobisii Culture B. globigii was grown for 72 hours at 35 ° C on Casitone nutrient agar plates (150 mm, Remel Co., Lenexa, Kansas) containing 0.002% MnCl 2 to induce approximately 80% sporulation. For each test, cells were harvested into 25 ml sterile phosphate buffer solution (PBS) and centrifuged at 3000 rpm for 15 minutes. The supernatant was decanted, and the cells were resuspended in 25 ml sterile PBS and vortexed thoroughly. The suspension was diluted to 0.1 O.D. 590nm (i.e., the suspension was diluted with PBS to 0.1 optical density at the 590 wavenumber) for dissemination using a Bausch and Lomb Spec-20 spectrophotometer. 2. Baseline Decay Characterization for B. slobigii A 0.1 O.D. 590nm suspension of 5. globigii was disseminated for 30 seconds using a BGI six jet collision nebulizer (CH Technologies, Westwood, Jew Jersey) at 40 psi in a Bioaerosol test chamber. The chamber air was sampled for 60 minutes at a rate of 50 L/min. using two New Brunswick Slit-to-Agar Biological Air Samplers (New Brunswick Scientific Co., Edison, New Jersey) with Casitone agar petri plates. The sampling began 1 minute after dissemination was stopped in order to allow the concentration of B. globigii to reach homogeneity in the chamber. A Climet CI-500 aerosol particle sizer (Climet Instrument Co., Redlands, California) was used to track the particle size distribution throughout the test (See Fig. 1). After the 60 minute sampling, the chamber air was purged clean, and the agar plants were removed and incubated for 15 hours at 35 ° C. Colonies were counted after the incubation period, and the baseline curve for B. globigii was established (See Fig. 2). 3. B. slobisii Dissemination Followed by a Low Concentration Powder Dispersion B. globigii was disseminated following the procedures described in Part 2 of this example. One minute after dissemination, sampling was commenced using the New Brunswick air samplers. Sampling was continued for 60 minutes. Five minutes after sampling was commenced, dispersion of Cl/AP-MgO powder (prepared as described in Example 2, Part 1) was initiated using a GEM-T air mill powder disperser (Coltec Industrial Products, Inc., Newtown, Pennsylvania) and a vibrating spatula (Mettler Toledo, Highstown, New Jersey). The powder was dispersed at a pressure of 40 psi until the concentration of powder in the air chamber reached approximately 4-5 mg/m 3 as indicated by a TSI Dustrak aerosol mass monitor (TSI, Inc., St. Paul, Minnesota). These results are shown in Fig. 3. The particle size distributions were tracked using the Climet CI-500 (See Fig. 4). At this powder concentration, the air mill was stopped. At the end of the 60 minute sampling period, the chamber air was purged clean, and the Casitone agar plates were removed and incubated for 15 hours at 35 °C. Colonies were counted after the incubations period and a decay curve for B. globigii was determined (See Fig. 5). 4. B. globigii Dissemination Followed by a High Concentration Powder Dispersion The procedure described in Part 3 of this example was repeated with the exception that the powder was dispersed to a concentration of approximately 20 mg/m 3 , as shown in Fig. 6. Fig. 7 sets forth the particle size distribution and Fig. 8 sets forth the decay curve for B. globigii with a high concentration of Cl/AP-MgO powder dispersion. 5. Results and Discussion The results of the tests conducted in Parts 2 -4 of this example are shown in Fig. 1-8. In Fig. 2, 5, and 8, the y-axis indicates the number of B. globigii colony forming units (CFU's) collected in 100 liters of air at the given time point indicated on the x- axis. CFU measurements of 200 indicate that there were too many CFUs to count, and thus the maximum number of 200 was assigned. In Fig. 2, the baseline decay curve indicates that the concentration of viable cells in the chamber remained relatively high, starting at above 200 CFU per 100 liters of air sampled and decreasing to approximately 65 CFU per 100 liters of air sample during an one hour period. In the presence of a low concentration of Cl/AP-MgO powder, the decay curve of B. globigii indicates that the CFUs started high at about 180 CFU per 100 liters of air sampled and decreased to less than 20 CFU per 100 liters of air sampled in about 23 minutes (Fig. 5). Finally, the decay curve of B. globigii in the presence of a high concentration of Cl/AP-MgO powder indicates that the CFUs started off very high at above 200 CFU per 100 liters of air sampled and decreased sharply to less than 20 CFU per 100 liters of air sample in about 20 minutes (Fig. 8). A comparison of the decay curves of B. globigii (Figs. 2, 5, and 8) indicates that the presence of metal oxide nanocrystals having reactive atoms stabilized on their surfaces has a significant impact on the number of viable cells recovered from the chamber environment. The data from Parts 3 and 4 above show that, as the concentrations of powder are increased, a more rapid decrease in the recovery of viable cells is obtained. EXAMPLE 4A Bacillus cereus bacterial endospores were grown and placed in water to form a suspension. A sterile nitrocellulose filter paper (3 cm diameter) was placed on a sterile rack, and 200μl of the aqueous spore suspension was distributed onto the filter paper. The filter was air dried for 2-4 hours. The dried filter paper was placed in a sterile beaker, and 10 ml of LB (Luria and Bertani) broth (containing 10 g/L tryptone, 5 g/L yeast extract, and 10 g/L sodium chloride, pH adjusted to 7 with 5 N NaOH, and sterilized by pressurizing to about 1500 psi) were placed in another sterile beaker. The latter beaker was covered with aluminum foil. One gram of CP-CaO, was spread on the filter paper so that all of the paper was covered, aluminum foil was placed on top of this beaker, and the beaker was allowed to stand for 2 hours. Using tweezers, the filter paper was removed and excess nanoparticle powder gently shaken off. The filter paper was immersed in the LB broth solution for 10 minutes with occasional swirling. Ten μl of the LB broth solution was extracted by a sterile syringe and distributed evenly on a Benzer agar culture plate using a sterile L-shaped glass piece. The lid was placed on the agar plate, and the sample was incubated for 12 hours at 37 °C. Three agar plates were prepared for each test. After incubation, the number of visible, living bacterial colonies was counted, and the percent killed or biocidally adsorbed (reduced) was determined using the following equations: Average Number of Colonies = n avg = (n, + n 2 + n 3 )/3 % of Microorganisms Reduced = n % = (r^ - n E )/n c x 100, where n E = average number of colonies on experimental plates, and where n c = average number of colonies on control plates. The above procedures were repeated using Cl/AP-MgO, I/AP-MgO, AP-CaO with vacuum dehydration, and AP-CaO with N 2 dehydration in place of CP-CaO. The results are set forth in Tables 1 and 2 below: Table 1 - Results of two hour a exposure - raw data. b a Refers to the time period beginning when the particular metal oxide powder was placed on the filter containing bacteria and ending when the filter was placed in the LB broth. b Numbers in parentheses are for repeated experiments with new reagents on a different day. c A negative number indicates enhanced growth. Table 2 - Results of two hour" exposure - raw data. a Refers to the time period beginning when the Br/AP-MgO powder was placed on the filter containing bacteria and ending when the filter was placed in the LB broth. EXAMPLE 4B This experiment was conducted to determine the effect of exposing B. cereus to nanocrystalline metal oxides for varying lengths of time. The procedure described in Example 4 was repeated using Cl/AP-MgO powder and contacting the Cl/AP-MgO powder with the B. cereus for 0 (control), 20, 40, 60, 80, and 100 minutes. The results of this set of tests are set forth in Table 3. Table 3 - Results of variable time exposure for Cl/AP-MgO adduct. a Refers to the time period beginning when the Cl/AP-MgO powder was placed on the filter containing bacteria and ending when the filter was placed in the LB broth. Discussion The results of the tests conducted in Examples 4 A and 4B confirm that Cl/AP- MgO is a very effective reagent for the biocidal destruction of B. cereus spores and supports the data reported in the previous examples above on the biocidal destruction of 5. globigii. Furthermore, Cl/AP-MgO acts rapidly, and even a 20 minute exposure was enough for efficient decontamination. Br/ AP-MgO and AP-CaO were also quite effective in their biocidal abilities. EXAMPLE 5 0.1 g of AP-MgO, 1/ AP-MgO, or Cl/AP-MgO was placed in a flask equipped with a magnetic stirrer with 100 ml of pentane. A VX chemical agent mimic, paraoxon (4.5 μl), was added to the flask, with 2 ml of the resulting sample being withdrawn and the UV spectrum taken at 2, 5, 10, 15, 20, 40, 60, 80, 100, and 120 minutes after addition of the paraoxon. These results are illustrated in Fig. 9 and indicate that all three of the metal oxide samples worked well at destructively adsorbing the paraoxon. Upon reaction with paraoxon, the color of the sample (AP-MgO) changed from slightly grayish to bright yellow. After the destructive adsorption of paraoxon was carried out, quantities of the AP-MgO/paraoxon samples were placed in solvents (methylene chloride or toluene) and sonicated for 30 minutes. After sonication, some of the liquid was removed from each sample and tested by GC-MS. The GC-MS results did not show the presence of paraoxon, thus providing evidence that the paraoxon was destructively adsorbed by the metal oxide samples. Similar results have been achieved when using non-modified nanocrystalline metal oxide particles to destructively adsorb 2-chloroethyl ethyl sulfide (2-CEES), diethyl-4-nitrophenylphosphate (paraoxon), and dimethylmethylphosphonate (DMMP) as reported in U.S. Patent Application No. 08/914,632 (continuation-in-part of U.S. Patent Application No. 08/700,221), incorporated by reference herein. EXAMPLE 6 In order to prepare metal oxide particles (e.g., AP-MgO, AP-CaO, etc.) having species adsorbed on the surfaces of the particles, 10 grams of the desired metal oxide is placed on a Schlenk flask. The air is evacuated, and the gaseous species is introduced. The sample is allowed to react for about 30 minutes, after which time the excess gaseous species is pumped out. Gaseous species which can be adsorbed on the surfaces of metal oxide particles include oxides of Group V and VI elements (such as NO 2 and SO 2 , respectively) and ozone.

Claims: We claim: 1. A method of sorbing a target substance comprising the steps of: (a) providing a quantity of a composite comprising metal oxide particles with a number of atoms different from those making up the metal oxide particles stabilized on the surfaces thereof, said atoms being selected from the group consisting of halogens and Group I metals and being present at a level of at least about 2 atoms per square nanometer of metal oxide surface area; and (b) contacting said composite with a target substance under condi- tions for sorbing at least a portion of said target substance, said target substance being selected from the group consisting of acids, alcohols, compounds having an atom of P, S, N, Se, or Te, hydrocarbon compounds, toxic metal compounds, bacteria, fungi, viruses, rickettsiae, chlamydia, and toxins. 2. The method of claim 1 , said atoms being present at a level of from about 3-8 atoms per square nanometer of metal oxide surface area. 3. The method of claim 1, said atom being selected from the group consisting of chlorine and bromine atoms. 4. The method of claim 1, said metal oxide particles being selected from the group consisting of MgO, CaO, TiO 2 , ZrO 2 , FeO, V 2 O 3 , V 2 O 5 , Mn 2 O 3 , Fe 2 O 3 , NiO, CuO, Al 2 O 3 , ZnO, and mixtures thereof. 5. The method of claim 1 , said target substance being a bacteria. 6. The method of claim 5, wherein said bacteria is gram positive. 7. The method of claim 6, said bacteria being selected from the group consisting of B. globigii and B. cereus. 8. The method of claim 1 , wherein said target substance is a hydrocarbon compound. 9. The method of claim 8, wherein said hydrocarbon compound is halogenated. 10. The method of claim 9, wherein said hydrocarbon compound is chlorinated. 11. The method of claim 1 , said particles having an average crystallite size of up to about 20 nm. 12. The method of claim 1 , said particles having a multi-point surface area of at least about 15 m 2 /g. 13. The method of claim 3 , wherein said atom is chlorine and said composite has a surface hydroxyl concentration of less than about 5 hydroxyl groups per square nanometer of metal oxide surface area. 14. The method of claim 1 , wherein said contacting step is carried out at a temperature of from about -40-600 °C. 15. The method of claim 1, wherein said contacting step takes place for at least about 4 seconds. 16. The method of claim 1, wherein said target substance is selected from the group consisting of bacteria, fungi, viruses, rickettsiae, chlamydia, and toxins, and said contacting step (b) results in at least a 90% reduction in the viable units of said target substance. 17. The method of claim 1 , wherein said target substance is selected from the group consisting of acids, alcohols, compounds having an atom of P, S, N, Se, or Te, hydrocarbon compounds, toxic metal compounds, and said contacting step (b) results in at least a 90% reduction in the concentration of said target substance. 18. A method of sorbing a target substance comprising the steps of: (a) providing a quantity of a composite comprising a self-sustaining body formed of a plurality of agglomerated metal oxide particles with a number of atoms different from those atoms making up the metal oxide particles stabilized on the surfaces thereof, said atoms being selected from the group consisting of halogens and Group I metals, and being present at a level of at least about 2 atoms per square nanometer of metal oxide surface area; and (b) contacting said composite with a target substance under condi- tions for sorbing at least a portion of said target substance, said target substance being selected from the group consisting of acids, alcohols, compounds having an atom of P, S, N, Se, or Te, hydrocarbon compounds, toxic metal compounds, bacteria, fungi, viruses, rickettsiae, chlamydia, and toxins. 19. The method of claim 18, wherein said particles are selected from the group consisting of MgO, CaO, TiO 2 , ZrO 2 , FeO, V 2 O 3 , V 2 O 5 , Mn 2 O 3 , Fe 2 O 3 , NiO, CuO, Al 2 O 3 , ZnO, and mixtures thereof. 20. The method of claim 18, said atoms being present at a level of from about 3-8 atoms per square nanometer of metal oxide surface area. 21. The method of claim 18, said atom being selected from the group consisting of chlorine and bromine atoms. 22. The method of claim 18, said target substance being a bacteria. 23. The method of claim 22, wherein said bacteria is gram positive. 24. The method of claim 23 , said gram positive bacteria being selected from the group consisting of B. globigii and B. cereus. 25. The method of claim 18, wherein said target substance is a hydrocarbon compound. 26. The method of claim 25, wherein said hydrocarbon compound is halogenated. 27. The method of claim 26, wherein said hydrocarbon compound is chlorinated. 28. The method of claim 18, said particles having surface-stabilized atoms being pressed at a pressure of from about 50 psi to about 6,000 psi. 29. The method of claim 18, wherein said particles having surface-stabilized atoms are pressed-together. 30. The method of claim 18, wherein said target substance is selected from the group consisting of bacteria, fungi, viruses, rickettsiae, chlamydia, and toxins, and said contacting step (b) results in at least a 90% reduction in the viable units of said target substance. 31. The method of claim 18, wherein said target substance is selected from the group consisting of acids, alcohols, compounds having an atom of P, S, N, Se, or Te, hydrocarbon compounds, toxic metal compounds, and said contacting step (b) results in at least a 90% reduction in the viable units of said target substance. 32. A method of sorbing a target substance comprising the steps of: (a) providing a quantity of a composite comprising metal oxide particles with a number of atoms different from those making up the metal oxide particles stabilized on the surfaces thereof forming a metal oxide-atom adduct, wherein when said adduct is heated to a temperature of about 100°C, less than about 10%> of the total weight loss of the adduct is attributable to the desorption of said atoms; and (b) contacting said composite with a target substance under condi- tions for sorbing at least a portion of said target substance, said target substance being selected from the group consisting of acids, alcohols, compounds having an atom of P, S, N, Se, or Te, hydrocarbon compounds, toxic metal compounds, bacteria, fungi, viruses, rickettsiae, chlamydia, and toxins. 33. The method of claim 32, wherein said atoms are selected from the group consisting of the halogens and Group I metals. 34. The method of claim 32, said atoms being present at a level of at least about 2 atoms per square nanometer of metal oxide surface area. 35. The method of claim 32, said particles being selected from the group consisting of MgO, CaO, TiO 2 , ZrO,, FeO, V 2 O 3 , V 2 O 5 , Mn 2 O 3 , Fe 2 O 3 , NiO, CuO, Al 2 O 3 , ZnO, and mixtures thereof. 36. The method of claim 32, said target substance being a bacteria. 37. The method of claim 36, wherein said bacteria is gram positive. 38. The method of claim 37, said gram positive bacteria being selected from the group consisting of B. globigii and B. cereus. 39. The method of claim 32, wherein said target substance is a hydrocarbon compound, and said hydrocarbon is halogenated. 40. The method of claim 39, wherein said hydrocarbon is chlorinated. 41. The method of claim 32, wherein said contacting step is carried out at a temperature of from about -40-600°C. 42. The method of claim 32, wherein said contacting step takes place for at least about 4 seconds. 43. The method of claim 32, wherein said target substance is selected from the group consisting of bacteria, fungi, viruses, rickettsiae, chlamydia, and toxins, and said contacting step (b) results in at least a 90% reduction in the viable units of said target substance. 44. The method of claim 32, wherein said target substance is selected from the group consisting of acids, alcohols, compounds having an atom of P, S, N, Se, or Te, hydrocarbon compounds, toxic metal compounds, and said contacting step (b) results in at least a 90%> reduction in the concentration of said target substance. 45. A method of sorbing a target substance comprising the steps of: (a) providing a quantity of finely divided nanocrystalline particles selected from the group consisting of metal oxides; and (b) contacting said particles with a target substance under conditions for sorbing at least a portion of said target substance, said target substance being selected from the group consisting of bacteria, fungi, viruses, rickettsiae, chlamydia, and toxins. 46. The method of claim 45, said particles being selected from the group consisting of MgO, CaO, TiO 2 , ZrO 2 , FeO, V 2 O 3 , V 2 O 5 , Mn 2 O 3 , Fe 2 O 3 , NiO, CuO, Al 2 O 3 , ZnO, and mixtures thereof. 47. The method of claim 45, said target substance being a bacteria. 48. The method of claim 47, wherein said bacteria is gram positive. 49. The method of claim 48, said gram positive bacteria being selected from the group consisting of B. globigii and B. cereus. 50. The method of claim 45, said particles having an average crystallite size of up to about 20 nm. 51. The method of claim 45, said particles having a multi-point surface area of at least about 15 m 2 /g. 52. The method of claim 45, wherein said contacting step is carried out at a temperature of from about -40-600°C. 53. The method of claim 45, wherein said contacting step takes place for at least about 4 seconds. 54. The method of claim 45, wherein the contacting step (b) results in at least a 90% reduction in the viable units of said target substance. 55. A method of sorbing a target substance comprising the steps of: (a) providing a quantity of a composite comprising particles of a first metal oxide selected from the group consisting of MgO, CaO, TiO 2 , ZrO 2 , FeO, V 2 O 3 , V 2 O 5 , Mn 2 O 3 , Fe 2 O 3 , NiO, CuO, Al 2 O 3 , ZnO, and mixtures thereof, said particles being at least partially coated with a quantity of a second metal oxide different from said first metal oxide and being an oxide of a metal selected from the group consisting of Ti, V, Fe, Cu, Ni, Co, Mn, Zn, and mixtures thereof; and (b) contacting said composite with a target substance under conditions for sorbing at least a portion of said target substance, said target substance being selected from the group consisting of bacteria, fungi, viruses, rickettsiae, chlamydia, and toxins. 56. The method of claim 55, said particles having an average crystallite size of up to about 20 nm. 57. The method of claim 55, said composite having a multi -point surface area of at least about 100 m 2 /g. 58. The method of claim 55, said first metal oxide comprising from about 90-99% by weight of the total composite material. 59. The method of claim 55, said second metal oxide comprising from about 1-1 % by weight of the total composite material. 60. The method of claim 55, said first metal oxide being MgO and second metal oxide being Fe 2 O 3 . 61. The method of claim 55 , said second metal oxide being applied as a thin layer having a thickness of up to about 1 nm. 62. The method of claim 55, said target substance being a bacteria. 63. The method of claim 62, said bacteria being gram positive. 64. The method of claim 63, said gram positive being selected from the group consisting of B. globigii and B. cereus. 65. The method of claim 55, wherein the contacting step (b) results in at least a 90% reduction in the viable units of said target substance. 66. A method of sorbing a target substance comprising the steps of: (a) providing a quantity of metal oxide particles having a species adsorbed on the surface thereof, said species selected from the group consisting of oxides of Group V elements, oxides of Group VI elements, ozone, and mixtures thereof; and (b) contacting said composition with a target substance under conditions for sorbing at least a portion of said target substance, said target substance being selected from the group consisting of bacteria, fungi, viruses, rickettsiae, chlamydia, and toxins. 67. The method of claim 66, wherein said species is an oxide of a Group V element. 68. The method of claim 67, wherein said oxide is NO 2 . 69. The method of claim 66, wherein said is an oxide of a Group VI element. 70. The method of claim 69, wherein said oxide is SO 2 . 71. The method of claim 66, wherein said species is ozone. 72. The method of claim 66, wherein said metal oxide particles are selected from the group consisting of MgO, CaO, TiO 2 , ZrO 2 , FeO, V 2 O 3 , V 2 O 5 , Mn 2 O 3 , Fe 2 O 3 , NiO, CuO, Al 2 O 3 , ZnO, and mixtures thereof. 73. The method of claim 66, said target substance being a bacteria. 74. The method of claim 73, wherein said bacteria is gram positive. 75. The method of claim 74, said bacteria being selected from the group consisting of B. globigii and B. cereus. 76. The method of claim 66, wherein said contacting step (b) results in at least a 90% reduction in the viable units of said target substance. 77. A composition comprising metal oxide particles with a number of atoms stabilized on the surface thereof, said atoms selected from the group consisting of the halogens, said atoms comprising at least two different halogens, and said atoms being present at a level of at least about 2 atoms per square nanometer of metal oxide surface area. 78. The composition of claim 77, wherein said metal oxide particles are selected from the group consisting of MgO, CaO, TiO 2 , ZrO 2 , FeO, V 2 O 3 , V 2 O 5 , Mn 2 O 3 , Fe 2 O 3 , NiO, CuO, Al 2 O 3 , ZnO, and mixtures thereof. 79. The composition of claim 77, wherein said at least two different halogens are selected from the group consisting of F and Cl, Br and Cl, I and Cl, F and Br, F and I, Br and I. 80. A method of sorbing a target substance comprising the steps of: (a) providing a quantity of the composition of claim 77; and (b) contacting said composite with a target substance under conditions for sorbing at least a portion of said target substance, said target substance being selected from the group consisting of acids, alcohols, compounds having an atom of P, S, N, Se, or Te, hydrocarbon compounds, toxic metal compounds, bacteria, fungi, viruses, rickettsiae, chlamydia, and toxins. 81. The method of claim 80, said metal oxide particles being selected from the group consisting of MgO, CaO, TiO 2 , ZrO 2 , FeO, V 2 O 3 , V 2 O 5 , Mn 2 O 3 , Fe 2 O 3 , NiO, CuO, Al 2 O 3 , ZnO, and mixtures thereof. 82. The method of claim 80, said target substance being a bacteria. 83. The method of claim 82, wherein said bacteria is gram positive. 84. The method of claim 83, said bacteria being selected from the group consisting of B. globigii and B. cereus. 85. The method of claim 80, wherein said target substance is a hydrocarbon compound, and said hydrocarbon is halogenated. 86. The method of claim 85, wherein said hydrocarbon is chlorinated.

Inventors: KLABUNDE KENNETH J, KOPER OLGA, KLABUNDE, KENNETH, J., KOPER, OLGA
Applicants: UNIV KANSAS STATE, KANSAS STATE UNIVERSITY RESEARCH FOUNDATION
Application year: 1999
Main classification: A62D
IPC codes: A01N25, A01N59, A61L2, A61L101, A62D3, A62D101, B01D53, B01J20, B82B1, C01F7, C01G9