SYNTHESIS OF BIOLOGICALLY ACTIVE COMPOUNDS IN CELLS

Abstract: This invention relates to a new method of synthesis of biologically active substances of determined structure directly in the cells of living organisms containing specific RNA or DNA molecules of determined sequence. The method is based on the hybridization of two or more oligomers bound with biologically inactive precursors of biologically active substances to specific RNA or DNA in vivo in the cells of living organisms. After hybridization of the oligomers to RNA or DNA the biologically inactive precursors bound to the 5' and/or 3' ends of the oligomers can interact with each other to make biologically active form of the substances. This changing of properties is due to chemical reactions which bind the biologically inactive precursors through a chemical bond into a biologically active form of the whole compound.

Description: Synthesis of biologically active compounds in cells. Technical field Int.Cl C07F 9/22; C07F 9/28; C07C 321/00; C07C 323/00 U.S. CI 560/147; 562/9; 562/10; 562/11 Field of search C07F 9/22 ; C07F 9/28 ; C07C 321/00; C07C 323/00 References cited U.S.patent documents 5, ,652, ,350 7/1997 Watanabe et al . , 55,, ,117777,, ,119988 11//11999933 Spielvogel et al., 5, ,594, ,121 1/1994 Froehler et al . , 5, ,599, ,922 2/1997 Grjasnov et al . , 5, 521, ,302 5/1996 Cook Ph.D, 5, ,177, ,064 1/1993 Bodor N.S. 55,, ,557711,, 993377 1111//11999966 Kyoichi A. Watanabe. OTHER REFERENCES Walder, J.A. , et al.,(1979), Complementary carrier peptide synthesis : General strategy and implications for prebiotic origin of peptide synthesis. Proc .Natl .Acad. Sci USA , vol.76, pp. 51-55. Ebata K. , et al.(1995), Nucleic acids hybridization accompanied with exci er formation from two pyrene-labeled probes. Photochemistry and Photobiology, vol. 62(5), pp. 836-839. Nielsen P.E. , (1995), DNA analogues with non phosphodiester backbones. Annu.Rev.Biophys . Biomol .Struct . vol.24, pp. 167-83. Tarn J.P., et al . , (1995), Peptide synthesis using unprotected peptides through orthogonal coupling methods . Proc.Natl.Acad. Sci.USA, vol.92, pp.12485-12489. Uhlmann G.A. et al . , (1990) Antisense Oligonucleotides : A New Therapeutic Principle, Chemical Rev., vol. 90, pp.543-584. Moser H.E. and Dervan P.B., (1987), Sequence-specific cleavage of double helical DNA by triple helix formation. Science, vol. 238, pp.645-650. Tulchinsky E. et al . , (1992) "Transcriptional analysis of the mts 1 gene with the specific reference to 5' flanking sequences. Proc.Natl.Acad. Sci USA , vol. 89, pp. 9146-9150. Background Art. The use of oligo (ribo) nucleotides and their analogues as anticancer and antiviruses theraupe ic agents was first proposed several years ago. (Uhlmann, 1990) The great number of different modifications of the oligonucieotides and the methods of their use has since been developed. Two basic interactions between oligonucieotides and nucleic acids are known (Moser and Dervan, 1987) 1. Watson-Crick base pairing (Duplex structure) 2. Hoogsten base pairing (Triplex structure) Oligonucieotides can form duplex and/or triplex structures with DNA or RNA of cells and so regulate transcription or translation of genes . It has been proposed that different: substances, which can cleave target nucleic acids or inhibit important cellular enzymes could be coupled to oligomers. The use of such conjugates as therapeutic agents has been described. (USA patent, 5,177,198; 5,652,350) . Other jnethods are based on the coupling of different biologically active substances, such as toxins, to monoclonal antibodies which can then recognise receptors or other structures of cancer cells, or cells infected with viruses. Monoclonal antibodies can then specifically recognise cancer cells and in this way transport toxins to these cells. But these methods are inefficient due to the high level of non-specific interactions between antibodies and other cells, which leads to delivery of the toxins or other biologically active compounds to the wrong cells. In 1979 I.M. Klotz and co-authors proposed a method for complementary carrier peptide synthesis based on a template- directed scheme (J.A. Walder et al. 1979) . The method proposed the synthesis of peptides on a solid support using unprotected amino acids, and the subsequent hybridization of oligonucieotides on the template. This method was established only for synthesis of peptides in vitro using solid supports of a different origin, and involved many synthesis steps to obtain peptides of the determined structure. RECTIFIED SHEET (RULE 91) ISA/EP M. Masuko and co-authors proposed another method for in vitro detection of specific nucleic acids by excimer formation from two pyrene-labeled probes (Ebata, K. et al . 1995). My invention allows the synthesis of different BACs of determined structure directly in living organisms only in cells, which have specific RNA or DNA sequences. In this way, BACs will be delivered only to those cells where specific nucleic acids are produced. Disclosure of Invention Definitions "roononuc1eoraer" The term "mononucleomer" means a "Base" chemically bound to "S" moieties. Mononucleomers can include nucleotides and nucleosides such as thymine, cytosine, adenine, guanine, diaminopurine , xanthine, hy oxanthine , inosine and uracil. Mononucleomers can bind each other to form oligomers, which can be specifically hybridized to nucleic acids in a sequence and direction specific manner. The "S" moieties used herein include D-ribose and 2 ' -deoxy- D-ribose. Sugar moieties can be modified so that hydroxyl groups are replaced with a heteroatom, aliphatic group, halogen, ethers, amines, mercapto, thioethers and other groups. The pentose moiety can be replaced by a cyclopentane ring, a hexose, a 6-member morpholino ring; it can be amino acids analogues coupled to base, bicyclic riboacetal analogues, morpholino carbamates, alkanes, ethers, amines, amides, thioethers, formacetals, ketones, carbamates, ureas, hydroxyiamines, sulfamates, sulfamides, sulfones, glycinyl amides other analogues which can replace sugar moieties. Oligomers obtained from the mononucleomers can form stabile duplex and triplex structures with nucleic acids . (Nielsen P.E. 1995, U.S.pat.No 5,594,121). "Base" "Base" (designated as "Ba") includes natural and modified purines and pyrimidines such as thymine, cytosine, adenine, guanine, diaminopurine, xanthine, hypoxanthine , inosine, uracil, 2-aminopyridine, 4, 4-ethanocytosine, 5-methylcytosine, 5- methyluracil, 2-aminopyridine and 8-oxo-N(6) -methyladenine and their analogues. These may include, but are not limited to adding substituents such as -OH, -SH, -SCH(3), -0CH{3), -F,-Cl,-Br, - RECTIFIED SHEET (RULE 91) ISA/EP NH(2), alkyl, groups and others. Also, heterocycles such as triazines are included. "Nucleotide" Nucleotide as used herein, means a base chemically bound to a sugar or sugar analogues having a phosphate group or phosphate analog. "oligomβr" Oligomer means that at least two "mononucleomers" (defined above) are chemically bound to each other. Oligomers can be oligodeoxyribonucleotides consisting of from 2 to 200 nucleotides, oligoribonucleotides consisting of from 2 to 200 nucleotides, or mixtures of oligodeoxyribonucleotides and oligoribonucleotides. The moncr-ucleomers can bind each other through phosphodies ter groups, phosphorothioate , phosphorodithioate, alkylphosphonate, boranophosphates , acetals, phosphoroamidate, bicyclic riboacetal analogues morpholino carbamates, alkanes, ethers, amines, amides, thioethers, formacetals, ketones , carbamates, ureas, hydroxylamines , sulfamates, sulfamides, sulfones, glycinyl amides and other analogues which can replace phosphodiester moiety. Oligomers are composed of mononucleomers or nucleotides . Oligomers can form stable duplex structures via Watson-Crick base pairing with specific sequences of DNA, RNA, mRNA, rRNA and tRNA in vivo in the cells of living organisms cr they can form stable triplex structures with double stranded DNA or dsRNA in vivo in the cells of living organisms. "Alkyl" "Alkyl" as used herein is a straight or branched saturated group having from 1 to 10 carbon atoms. Examples include methyl, ethyl, propyl, isopropyl, butyl, isobutyl, tert-butyl, pentyl , hexyl and the like. "Alkenyl" "Alkenyl" as used herein is a straight- or branched-chain olefinically-unsaturated group having from two to 25 carbon atoms. The groups contain from one to three double bounds. RECTIFIED SHEET (RULE 91) ISA/EP Examples include vinyl ( -CHdbdCH ( 2 ) , 1-propenyl ( -CHdbdCH- CH ( 3 ) ) , 2 -me thyl-l-propenyl ( -CKάbdC ( CH ( 3 ) ) -CH ( 3 ) ) and the like "Alkynyl" "Alkynyl" as used herein is a straight or branched acetynically-unsaturated group having from two to 25 carbon atoms . The groups contain from one to three triple bounds . Examples include 1-alkynyl groups include ethynyl (-CtbdCH), 1- propynyl (-CtbdC-CH (3 ) ) , 1-butynyl (-CtbdC-CH(2 -CH(3)), 3- methyl-butynyl ( -CtbdC-CH (CH (3 ) ) -CH (3 ) ) , 3 , 3-dimethyl-butynyl (- CtbdC-C(CH(3) ) (3) ) , 1-pentynyl ( -CtbdC-CH (2 , -CH(2 -CH(3)) and 1,3-pentadiynyl ( -CtbdC-CtbdC-CH (3 ) ) and the like. "Aryl" "Aryl" as used herein includes aromatic groups having from 4 to 10 carbon atoms. Examples include phenyl , naphtyl and like this. "Heteroalkyl" "Heteroalkyl" as used herein is an alkyl group in which 1 to 8 carbon atoms are replaced with N (nitrogen) , S (sulfur) or 0 (oxygen) atoms. At any carbon atom there can be one to three substituents . The substituents are selected from: -OH, -SH, - SCH3, -OCH3, halogen, -NH2 , O2 , -S(O)-, -S(0)(0)-, -0-S(0)(O)- 0-, -O-P(O) (0) -0-, -NHR and -R. Here R is alkyl, alkenyl, aryl, heteroaryl, alkynyl, heterocyclic, carbocyclic and like this groups . "Heteroalkenyl" "Heteroalkenyl" as used herein is an alkenyl group in which 1 to 8 carbon atoms are replaced with N (nitrogen) , S (sulfur) or 0 (oxygen) atoms. At any carbon atom there can be one to three substituents. The substituents are selected from group - OH, -SH, -SCH3, -OCH3, halogen, -NH2 , NO2 , -S(0)-, -S(0)(0)-, - 0-S(0) (O)-O-, -O-P(O) (0) -0-, -NHR and -R. Here R is alkyl, alkenyl, aryl, heteroaryl, alkynyl, heterocyclic, carbocyclic and like this groups. "Heteroalkynyl" RECTIFIED SHEET (RULE 91) ISA/EP "Heteroalkynyl " as used herein is an alkynyl group in which 1 to 8 carbon atoms are replaced with N (nitrogen) , S (sulfur) or 0 (oxygen) atoms. At any carbcn atom there can be one to three substituents. The substituents are selected from group -OH, - SH, -SCH3, -OCH3, halogen, -NH2 , O2 , -S(O)-, -S(0)(0)-, -0- S (0) (0) -0-, -0-P(0) (0) -0-, -NHR. Here R is alkyl, alkenyl, aryl, heteroaryl, alkynyl, heterocyclic, carbocyclic and like this groups . "Heteroaryl" "Heteroaryl" as used herein means an aromatic radicals comprising from 5 to 10 carbcn atoms and additionally containing from and to three heteroatoms in the ring selected from group S, 0 or N. The examples include but not limited to: furyl , pyrrolyl, imidazolyl, pyridyl indolyl, quinolyl, benzyl and the like. One to three carbon atoms of aromatic group can have substituents selected from -OH, -SH, -SCH3 , -OCH3 , halogen, -NH2 , NO2 , -S(0)- ,-S(0) (0)-, -0-S(0) (O)-O-, -O-P(O) (O)-O-, -NHR, alkyl group . Here R is alkyl, alkenyl, aryl, heteroaryl, alkynyl, heterocyclic, carbocyclic or similar groups. "Cycloheteroaryl" "Cycloheteroaryl" as used herein means a group comprising from 5 to 25 carbon atoms frcrr. one to three aromatic groups which are combined via a carbocyclic or heterocyclic ring. An illustrative radical is fluorenylmethyl . One to two atoms in the ring of aromatic groups can be heteroatoms selected from N, 0 or S. Any carbcn atom of the group can have substituents selected from -OH, -SH, -SCH3, -OCH3 , halogen, -NH2 , NO2 , -S (0) -, -S (0) (0) -, -O-S (0) (0) -0-, -0-P(0) (0) -0-, -NHR, alkyl group. Here R is alkyl, alkenyl, aryl, heteroaryl, alkynyl, heterocyclic and carbocyclic and like this groups. "Carbocyclic" "Carbocyclic" as used herein designates a saturated or unsaturated ring comprising from 4 to 8 ring carbon atoms. Carbocyclic rings or groups include cyclopentyl, cyclohexyl and phenyl groups. Any carbcn atom of the group can have substituents selected from -OK, -SH, -SCH3, -OCH3 , halogen, -NH2 , N02, -S(O)-, -S(O) (0) -, -O-S(C) (O)-O-, -O-P(O) (O)-O-, -NHR, alkyl group and R. Here R is alkyl, alkenyl, aryl, heteroaryl, alkynyl, hetsrocyclic and carbocyclic and like this groups. "Heterocyclic ring" "Heterocyclic ring" as used herein is a saturated or unsaturated ring comprising from 3 to 8 ring atoms. Ring atoms include C atoms and from one to three N, 0 or S atoms. Examples include pyri idinyl , pyrrolinyl, pyridinyl and morpholinyl. At any ring carbon atom there can be substituents such as -OH, -SH, -SCH3, -OCH3, halogen, -NH2. O2 , -S(0)-, -S(0)(0)-, -0-S(0)(0)- 0-, -O-P(O) (0) -0-, -NHR, alkyl. Where R is alkyl, alkenyl, aryl, heteroaryl, alkynyl, heterocyclic and carbocyclic and like this groups. "Hybridization" "Hybridization" as used herein means the formation of duplex or triplex structures between oligomers and ssRNA, ssDNA, dsRNA or dsDNA molecules. Duplex structures are based on Watson-Crick base pairing. Triplex structures are formed through Hoogsteen base interactions. Triplex structures can be parallel and antiparallel . The word "halogen" means an atom selected from the group consisting of F (fluorine) , CI (clorine) , Br (bromine) and I (iodine) The word "hydroxyl" means an --0H group. The word "carboxyl" means an -- COOH function. The word "mercapto" means an -- SH function. The word "amino" means --NH(2) or —NHR. Where R is alkyl, alkenyl, aryl, heteroaryl, heteroalkyl, alkynyl, heterocyclic, carbocyclic and like this groups. "Biologically active compounds (BACs)" "Biologically active compound as defined herein include but are not limited to: 1) biologically active peptides and proteins consisting of natural amino acids and their synthetic analogues , D, or DL configuration at the alpha carbon atom selected from valine, leucine, alanine, glycine, tyrosine, tryptophan, tryptophan RECTIFIED SHEET (RULE 91) I5A EP isoleucine, proline, histidine, lysin, gluta ic acid, methionine, serine, cysteine, glutamine phenylalanine, methionine sulfoxide, threonine, arginine, aspartic acid, asparagin, phenylglycine, norleucine, norvaline, alpha-aminobutyric acid, O-methylserine, O-ethylserine, S-methylcysteine, S-benzylcysteine, S- ethylcysteine , 5 , 5 , 5-trifluoroieucine and hexafluoroleucine. Also included are other modi ications of amino acids, which include but are not limited tc, adding substituents at carbon atoms such as -OH, -SH, -SCH3, -OCH3 , -F,-Cl,-Br, -NH2. The peptides can be also glycosylated and phosphorylated. 2) Cellular proteins which include but are not limited to: enzymes, DNA poly erases, RNA polymerases, esterases, lipases, proteases, kinases, transferases , transcription factors, transmembrane proteins, membrane proteins, cyclins, cytoplasmic proteins, nuclear proteins, toxins and like this. 3) Biologically active RNA such as mRNA, ssRNA, rsRNA and like this. 4) Biologicc.lly active alkaloids and their synthetic analogues with added substituents at carbon atoms such as -OH, -SH, -SCH3, -OCH3 , -F,-Cl,-Br, -NH2, alkyl straight and branched. 5) Natural and synthetic organic compounds which can be: a) inhibitors and activators of the cellular metabolism; b) cytolitical toxins; c) neur toxins; d) cofactors for cellular enzymes; e) toxins; f) inhibitors of the cellular enzymes. "Precursor(s) of biologically active substances (PBAC(s))" "Precursors of biologically active compounds (PBACs) " as used herein are biologically inactive precursors of BACs which can form whole BACs when bound to each other through chemical moiety(ies) "m" or simultaneously through chemical moieties "m" and "m Λ l". ".ti" and "m Λ l" are selected independently from: -S-S-, -0-, -NH-C(O)-, -C(0)-NH-, -C(O)-, - H- , dbdN- , -C(0)0-, -C(0)S-, -S-, -C(S)S-, -C(S)C~, -N=N-. Biologically active peptides and proteins are synthesized from shorter biologically inactive peptides. These shorter peptides as used herein are also biologically inactive precursors of biologically active compounds. RECTIFIED SHEET (RULE 91) ISA/EP Biologically active RNAs can be synthesized from biologically inactive oligoribonucleotides. "oligomer-PBAC" "Oligomer-PBAC" as used herein means a precursor of a BAC (PBAC) which is chemically bound at the first and/or last mononucleomer at the 3' and/or 5' ends of the oligomer through the chemical moieties L Λ 1 and/or IA2. Chemical moieties L Λ 1 and L Λ 2 can be bound directly to a base or to a sugar moiety or to sugar moiety analogues or to phosphates or to phosphate analogues, w oligomer n -PA n " "Oligomer n -PA n " as used herein means the precursor of a biologically active protein or RNA which is chemically bound at the first and/or last mononucleomer at the 3' and/or 5' ends of the oligomer through the chemical moieties L Λ 1 and/or L Λ 2. n means the ordinal number of the oligomer of PA. PAs are biologically inactive peptides or biologically inactive oligoribonucleotides. Wherein n is selected from 2 to 300. a) In Formulas from 1 to 4 PBACs are designated as "A" and "B" . A-m-B is equal to a whole BAC "T" "m" is selected independently from -S-S-, -0- , -NH-C(O)-, -C(0)-NH-, - (O)-, -NH-, dbdN-, -C(0)0-, -C(0)S-, -S-, -C(S)S-, -C(S)0-, -N= -. A-O-B is equal to a whole BAC "T" A-NH-C(0)-B is equal to a whole BAC "T A-C(0)-NH-B is equal to a whole BAC "T A-C(0)-B is equal to a whole BAC "T" A-NH-B is equal to a whole BAC "T" A-dbd —B is equal to a whole BAC "T" A-C(0)0-B is equal to a whole BAC "T n A-C(0)S-B is equal to a whole BAC "T A-C(S)S-B is equal to a whole BAC "T" A-S-S-B is equal to a whole BAC "T A-C(S)0-B is equal to a whole BAC "T" A-N=N-B is equal to a whole BAC "T" RECTIFIED SHEET (RULE 91) ISA/EP b) Biologically active compounds can be formed through moieties " " and "m Λ l". " " and "πAl" are selected independently from: -S-S-, -0-, -NH-C(O)-, -C(0)-NH-, -C(O)-, -NH- , dbdN- , - C(0)0-, -C(0)S-, -S-, -C(S)S-, -C(S)0, -N=N-, so that a BAC is represented on figure 4. c) In Formulas from 5 to 7 , precursors of BACs (PBACs) are designated as "PA n A where n is selected from 2 to 300. "PA" are peptides consisting of frcr 2 to 100 amino acids or oligoribonucleotides consisting of from 2 to 50 ribonucleotides . {PAι-m-PA2-m-PA3-m-... -m-PA n -3-πι-PA n _2-m-PA n -i-m-PA n } is equal to BAC. BACs in this case are proteins or RNAs. Proteins can be enzymes, transcription factors, ligands, signaling proteins, transmembrane proteins, cytolitical toxins, toxins, cytoplasmic proteins, nuclear proteins and the like. Detailed disclosure of the invention This invention relates to the synthesis of biologically active compounds directly into the cells of living organisms. This is achieved by the hybridization of two or more oligomers to cellular RNA or DNA. These oligomers are bound to biologically inactive PBACs (oligomer-PBACs) containing chemically active groups . BAC can be synthesized only in those cells of living organisms which have specific RNA or DNA molecules of a determined sequence. The principle Formulas of the invention are represented below: RECTIFIED SHEET (RULE 91) ISA/EP Formula 1 . A - KM K A 2-B I L A 1 L A 2 5' <3' ) Oligomer A 3 . ( 5 . ) 5 ' ( 3 ' ) Oligomer B 3 ' < 5 ' cellular RNA, DNA after hybrid ization the d istance chemical bond formation between the ends of theoligomers is from 0 to 7 nucleotides degradation of oligomers and/or linking moieties and release of BAC ^directly into targeted cells "A-πrB" is the biologically active compound After hybridization of the "Oligomer-PBACs" "A" and "B" to cellular RNA, DNA or dsDNA, the chemically active groups K 1 and K Λ 2 of the oligomer-PBACs "A" and "B" interact with each other to form the chemical moiety "m" , which combines PBACs "A" and "B" into one active molecule of biologically active compound "T" . The degradation of the oligomers and/or linking moieties L 1 and L Λ 2 by cellular enzymes or hydrolysis leads to the release of the synthesized BAC "T" directly int-o the targeted cells. After hybridization of the oligomer-PBACs to cellular RNA or DNA the distance between the 3 ' or 5 ' ends of the oligomer A and 5 ' or 3 ' ends of the oligomer B is from 0 to 7 nucleotides of cellular RNA, DNA or dsDNA. Formula 2 Λ 2-_ I (3' ) OϋgomerA L Λ 1 3' (5' ) 15' (3 OϋgomerB 3' (5' cellular 3' (5' 5' (3 -' )RNA, DNA Ihybridization of "Oligomer-PBACs 1 :o cellular RNA, DNA ater yr zation the distance and release of PBAC "A" between the ends of the oligomers from "Oligomer A" is from 0 to 7 nucleotides of cellular RNA or DNA A-m -B I cellular 5<(3<) OligomerA S S L ∑I_ OligomerB 3 AS ■Mi n i i ~ JED i MI II II RNA, DNA 3' (5') 5'(3') degradation of oligomers and/or linking moiety and subsequent release of BAC directly into targeted cells "A-m-B" is the biologically active compound T After hybridization of the "oligomer-PBACs" "A" and "B" to cellular RNA, DNA or dsDNA the chemically active group K Λ 2 of the oligomer-PBAC "B" interacts with the linking moiety L Λ 1 of the oligomer-PBAC "A" to combine the PBACs through the chemical moiety "m" into one active molecule of biologically active compound "T" with the subsequent release of one PBAC "B" from the oligomer. The degradation of the oligomer and/or linking moieties IA1 by cellular enzymes or hydrolysis leads to the release of synthesized BAC "T" directly ir.ro the targeted cells. Formula 3. A -K" 5' (3' OligomerA L Λ 1 '2 3' (5' 5' < 3, ) OligomerB 3' (5' cellular 3' (5' ) 5 '< 3 '> RNA, DNA hybridization of "Oligomer-PBACs" to cellular RNA, DNA A _K Λ l of cellular RNA or DNA activated L A 2 moiety interacts with A 1 moiety T v.ith release of BAC from oligomer directly into targeted cells. "A-m -B" is equal :o the biologically active compound "T" The chemically active group K Λ 1 of the oligomer-PBAC A interacts with the linking moiety IA2 to combine the PBACs through the chemical moiety "~" into one active molecule of the biologically active compound "T" with the subsequnet release of one PBAC "B" from oligomer "B" and the activation of the chemical moiety IA2. After activation, LA2 interacts with the linking moiety LAI to release the biological compound "T" from the oligomer directly into targeted cells. Formula . (5' cellular RNA, DNA 3' (5') 5' (3') hybridization of "Oligomer-PBACs" \ tc cellular RNA or DNA A _K Λ 1 K Λ 2-B ' . L' 2 cellular 5 ' (3,) OliαomerA j ) 5 ' (3 '> Oligomer B 3 ' (5 ' ) m I I M I i I I H l l l l l l l l l l ' 3' {5') 5'(3') chemical bonds formation \ and release of PBACs "A" and "B" from oligomers A and B ologically active compound "T" After hybridization of the "oligomer-PBACs" "A" and "B" to cellular RNA, DNA or dsDNA, the chemically active group K Λ 2 of the oligomer-PBAC "B" interacts with the linking moiety L Λ 1 of the oligomer-PBAC "A" to combine the PBACs through the chemical moiety "m" . At the same time the chemically active group K Λ 1 of the oligomer-PBAC "A" interacts with the linking moiety L Λ 2 of the oligomer-PBAC "B" to form chemical moiety m Λ l. Which together with chemical moiety m combines two "Oligomer-PBACs" into one active molecule of biologically active compound "T", with the release of BAC from the oligomer. Formula 5. 3' [C I 5'(3' I :.yc::α:zatιon of "Oligomer-PA'n's cellular to RNA or DNA directly in ceils RNA, DNA (N= from 0 to 7) r » pA » m » PA » — m — "?A"- — m — ■ ■ ■ _ m —"PA" ) is i I 2 - n J biologically active compound "PR" After simultaneous hybridization of "Oligoxnern-l-PA n -l" and "Oligo ern-PAn" to cellular RNA or DNA, the chemically active groups K Λ 1 and K Λ 2 interact with each other to form the chemical moiety "m" between "Oligomern-i-PAn-i" and "01igomer n -PA n " correspondingly; This step is repeated in the cells n-1 times and combines n-1 times all "PA n "s into one active molecule of the biologically active compound "PR" which consists of n PA n so that compound {"PA"ι-m-"PA"2-m-"PA"3-m-"PA"4-m-... -m-"PA n -3 "-m-"PA n -2"-ra- "PA n _i " -m-"PA n" } is biologically active compound "PR". The degradation of the oligomers and/or linking moieties L 1 and L Λ 2 leads to the release of the synthesized BAC "PR" directly into targeted cells of living organism. Here, n is selected from 2 to 2000; Formula 6. w H--PA" κ -; - n Oligoieriy loiigomer2J 5'(3'1 3' ( 5' ) 5 . ( r ( 5' } 3' (5') 5 (N= from C to 7) 'PA » m -P " 2 — m — "PA" 3 — m - i n — m — -PA' j S biologically active compound "PR" After simultaneous hybridization of "oligomer n -i-PA n _ι« and "oligomer n -PA n " to cellular RNA, DNA or dsDNA, the chemically active group K Λ 1 of "oligomer n -PAn" interacts with the linking moiety L Λ 2 of "oligomer n _ι-PA n _ι» to bind PA n _χ and PA n through chemical moiety "m" . This step is repeated in the cells n-1 times and combines n-1 times all PA n s after hybridization of all n " oligomer-PAn " s into one active molecule of the biologically active compound "PR", which consists of n PAs so REC Ϊ-O 5 ' :::τ ;AL≡ 91) ISA/EP that compound {PAι-m-PA2-m-PA3-rr.-PA4-m- ... -m-PA n -3-m-PA n -2-m-PA n - l-m-PA n } is equal to the biologically active compound PR. The degradation of the oligomers and/or linking moieties L 1 by cellular enzymes or hydrolysis leads to the release of the synthesized BAC PR directly into targeted cells of living organism, here n is selected from 1 to 2000; Formula 7. •pi" m »ps» — m — »:-" — m — ■ ■ ■ — m — "PI" 5- 3-(5') 5 , (3 ., cellular RΝA or DA After simultaneous hybridization of "Oligomer n _i-PA n -i " and "oligomer n -PA n " to cellular RNA, DNA or dsDNA, the chemically active group K A 1 of "oligomer n _ι-PA n -i" interacts with the linking moiety IA2 of "oligomer n -PA n " to bind PA n _ι and PA n through chemical moiety "m" . After interaction of K Λ 1 with LA2, IA2 is chemically activated so that it can interact with linking moiety IA1 of the oligomer-PA n _ ( thus destroying the binding of the oligomer n -i to PA n -l- This process is repeated n-1 times, so that only whole BAC "PR" comprising from n PA n s {PA_-m-PA2-m-PA3- m-PA4~m- ... -m-PA n -3-m-PA n -2-m-PA n -i-m-PA n } is released directly into the targeted cells of living organisms, here n is selected from 2 to 2000. The chemical moieties in the Formulas 1,2,3,4,5,6 and 7 are as follows: m is selected independently from: -S-S-, -N(H)C(0)-, - C(0)N(H)-, -C(S)-0-, -C(S)-S-, -0-, -N=N-, -C(S)-, -C(0)-0-, -NH-, -S-; K Λ 1 is selected independently from: -NH(2) , dbdNH, -OH, -SH, -F, -CI, -Br, -I, -R Λ 1-C (X) -X Λ 1-R Λ 2 ; K 2 is selected independently from: - NH(2), -dbd-NH, -OH, SH, -R 1-C(X)-X Λ 1-R Λ 2, -F, -Cl, -Br, -I; L Λ 1 is independently: chemical bond, -R Λ 1-, -R A l-0-S-R Λ 2- , -R Λ l-S-0-R Λ 2-, -R Λ 1-S-S-R Λ 2-, -R Λ 1-S-N (H) -R Λ 2- , -R 1-N(H)-S-R Λ 2-, -R Λ l-0-N(H)-R Λ 2-, -R Λ 1-N (H) -0-R Λ 2- , -R Λ 1-C(X)-X Λ 1-R Λ 2-; L A 2 is independently: chemical bond, -R Λ 1-, -R Λ l-0-S-R A 2-, -R Λ l-S-0-R Λ 2-, -R Λ 1-S-S-R Λ 2-, -R Λ 1-S-N (H) -R Λ 2- , -R Λ 1-N(E)-S-R Λ 2-, -R Λ l-0-N(H) -R Λ 2-, -R Λ 1-N (H) -0-R Λ 2- , -R Λ 1-C(X)-X Λ 1-R Λ 2-, -R 1-X-C(X)-X-C(X)-X-R Λ 2-; R Λ 1 is independently: chemical bond, alkyl, alkenyl, alkynyl, aryl, heteroalkyl, heteroalkenyl , heteroalkynyl , heteroaryl, cycloheteroaryl, carbocyclic, heterocyclic ring, X Λ 1-P(X) (X)-X Λ 1, -S(O)-, -S(0)(0)-, -X 1-S(X) (X)-X 1-, C(O)-, -N(H)-, -N=N-, -X~1-P(X) (X)-X Λ 1-, -X Λ 1-P (X) (X) -X Λ 1- RECTIFIED SHEET (RULE 91) ISA/EP P(X)(X)-X Λ 1, -X Λ 1-P(X) (X) -XΑ-P(X) (X) -X 1-P(X) (X)-X 1, C(S)-, any suitable linking group; R Λ 2 is independently chemical bond, alkyl, alkenyl, alkynyl, aryl, heteroalkyl, heteroaikenyl, heteroalkynyl , heteroaryl, cyclohe~eroaryl, carbocyclic, heterocyclic ring, X Λ 1- P(X)(X)-X Λ 1, -S(O)-, -S(0) (0)-, -X Λ 1-S(X) (X)-X Λ 1-, -C(O)-, - N(H)-, -N=N-, -X Λ 1-P(X) (X) -X Λ 1-, -X 1-P (X) (X) -X Λ 1-P (X) (X) - X Λ 1, -X 1-P(X) (X)-X 1-P(X) (X)-X Λ 1-P(X) (X)-X 1, -C(S)-, any suitable linking group; X is independently S, 0, NH, Se, alkyl, alkenyl, alkynyl; X Λ 1 is Independently S, 0, NH, Ξe, alkyl, alkenyl, alkynyl. In Formulas 1,2,3,4,5,6 and 7 the linking moieties 1 and IA2 are bound to the first and/or last mononucleomers of the oligomers at their sugar or phosphate moiety, or directly to base, or to sugar moiety analogues, or to phosphate moiety analogues, or to base analogues. All the described schemes demonstrate that BACs can not be synthesized in non-targeted cells because the molar concentration of the chemically active groups is too low, and without hybridization of the oligomer-PBACs to the template, specific reactions cam. not occur. After hybridization of the oligomer- PBACs to a specific template, the concentration of the chemically active groups is sufficient for the chemical reaction between the chemical groups of PBACs to occur. The reaction leads to chemical bond formation between PBACs and subsequent formation of a whole BAC. The degradation of the oligomers and/or linking moieties of the oligomers with PBACs leads to the release of BACs directly into targeted cells. To synthesise biologically active polymers such as proteins and RNAs of determined structure directly into cells more than two PBACs can be used. PBACs for synthesis of proteins or RNAs are designated as PA n . Pa are peptides or oligoribonucleotides. The mechanisms of the interaction of such PBACs are the same as in the synthesis of small biologically active compounds. The difference is that the PBACs (with the exception of the first and last PBACs) are bound simultaneously RECTIFIED SHEET (RULE 91) ISA/EP to the 5' and 3' ends of the oligomers so that the direction of synthesis of the biologically active protein or RNA can be determined. Possible functions of BACs synthesized by proposed methods are: 1} Killing of cells, 2) Stimulation of the metabolism of cells 3) Blocking of important ion channels such as Na + , K + , Ca ++ and other ion channels, in order to inhibit signal transmissions. BACs can be proteins, peptides, alkaloids and synthetic organic compounds. They can be cleaved into two or more precursors called PBACs . After interaction between the chemical groups of PBACs, whole BAC is formed through the moiety " " . a) In Formula 1,2,3 and 4 PBACs are designated as "A" and "B" A-"m"-B is equal to a whole BAC "m" is selected independently from -S-S-, -0-, -NH-C(O)- C(0)-NH-, -C(O)-, -NH-, dbdN-, -C(0)0-, -C(0)S-, -S-, -C(S)S- C(S)0, -N=N-. A-o-B is equal to a whole BAC A-NH-C(0)-B is equal to a whole BAC it in II A-C(0)-NH-B is equal to a whole BAC n m n A-c(o)-B is equal to a whole BAC II m n A-NH-B is equal to a whole BAC II m il A-dbdN---B is equal to a whole BAC it m n A-c(o)o-B is equal to a whole BAC li it A-c(o)s-B is equal to a whole BAC ti m n A-c(S)s-B is equal to a whole BAC n mil A-s-s-B is equal to a whole BAC II m n A-c(S)o-B is equal to a whole BAC it m n A-N=N-B is equal to a whole BAC II m n b) A biologically active compound can be formed through the moieties "m" and "m Λ l". "m" and "m Λ l" are selected independently from: -S-S-, -0- , -NH-C(O)-, -C(0)-NH-, -C(0)-, - NH-, dbdN-, -C(0)0-, -C(0)S-, -S-, -C(S)S-, -C(S)0, -N=N- , so that RECTIFIED SHEET (RULE 91) ISA/EP This kind of interaction is represented in figure 4. c) In Formulas 5, 6 and 1 , precursors of BACs (PBACs) are designated as "PAnA where n is selected from 2 to 2000. "PA" are peptides or oligoribonucleotides consisting of from 2 to 100 amino acids or ribonucleotides correspondingly, n is the ordinal number of PA in a series of PAs and designates the sequence of binding of PAs to each other. { "PAl " -m- "PA . 2 " -m- "PA3 " -m- . - -m- "PAn-3 " ~ ~ "PAn-2 " -m- "PAn-1 " -πt- "PAn") is equal to BAC "PR". BACs "PR" in this case are proteins or RNAs. Proteins can be cellular proteins, enzymes, transcription factors, ligands, signalling proteins, transmembrane proteins, cytolitical toxins, cytoplasmic and nuclear proteins and the like. RNAs are selected from mRNA, rsRNA and the like. Brief description of drawings. Fig.l Synthesis of the toxin daphnoretin. Toxin Daphnoretin is cleaved into two precursors. After simultaneous hybridization to cellular RNA of the oligomers bound to the daphnoretin' s precursors, the chemically active hydroxyl group of daphnoretin' s precursor "A" interacts with the chemically active CI group of precursor "B" to form a chemical bond between two daphnoretin precursors . The degradation of the linking moieties and/or oligomers leads to the release of the biologically active molecule directly into targeted cells. Fig.2 Synthesis of the neurotoxin peptide, Neurotoxin is cleaved into two shorter, biologically inactive peptides . After hybridization to cellular RNA or DNA, the chemically active NH2 group of peptide "A" interacts with the linking moiety -C (0) -0-L 2 , forming a peptidyl bond. After the peptidyl bonds formation, the chemically active group -SH of peptide "B" interacts with the linking moiety L A 1-S-S- which binds peptide "A" with oligomer "A" - After this interaction, an - S-S- bound between the two cysteins is formed and the biologically active neurotoxin is released into targeted cells. Amino acids are designated as italicised letters in one letter code. Fig.3 Tne synthesis of the toxin tulopsoid A. RECTIFIED SHEET (RULE 91) ISA/EP Toxin tulopsoid A is cleaved into two precursors . After simultaneous hybridization to cellular RNA of the oligomers bound to the tulopsoid A precursors chemically active hydroxyl group of the oligcmer-PBAC "A" interacts with the -CH2-S-C(0)- linking moiety to form a chemical bond with tulopsoid' s precursor "B" , releasing precursor "B" from oligomer 2. The activated -CH2-SH moiety interacts with the linking moiety -S-0-, releasing the whole tulopsoid A from oligomer 1. Fig.4 Synthesis of the toxin amanitin. Toxin-amanitin is a strong inhibitor of transcription. It can be cleaved into two inactive precursors, which can be used to synthesise the whole molecule of amanitin. After hybridization of all oligomers bound with the amanitin' s precursors to cellular RNA or DNA, free amino group of amanitin 's precursor "A" can interact with the carboxyl group -C(0)-S-L A 2 to form a peptidyl bond and to release amanitin 's precursor "B" from oligomer 2. The linking moiety of amanitin ' s precursor "A" to the oligomer 1 is semistabile. The release of precursor "A" from the oligomer 1 is performed due to action of the activated -SH group on the linking moiety -C (0) -0-S-L A l . Oligomers 3 and 4 bound with the amanitin' s precursors "A" and "B" are hybridized on the same molecule of RNA or D A. The amino group of amanitin 's precursor "B" interacts with the carboxyl group -C(0)-S-L 1 to form a peptidyl bond, releasing amanitin 's precursor "A" from the oligomer 3. The linking moiety of amanitin' s precursor "B" to the oligomer 4 is semistabile. The release of precursor "B" from the oligomer 4 is performed due to action of the activated -SH group on the linking moiety -C (0) -0-S- 2. Fig.5 Synthesis of the toxin D-actinomicin. Toxin D-actinomicin is cleaved into two precursors. After simultaneous hybridization of two oligomer-PBACs to cellular RΝA or DΝA chemically active amino and halogen groups of precursor "A" interact: with the chemically active halogen and hydroxyl groups of D-actinomicin ' s precursor "B" respectively to form two chemical bonds between the precursors . Fig.6 Synthesis of the toxin ochratoxin A. Toxin ochratoxin A is cleaved into two precursors, which are bound to oligomers. After simultaneous hybridization of the oligomer-PBACs to cellular RNA or DΝA, the chemically active amino group of precursor "B" interacts with the moiety C(0)-0- RECTIFIED SHEET (RULE 91) ISA/EP which links precursor "A" with oligomer A, to form a chemical bond between the two ochratoxin precursors. After oligomer or linking moiety degradation in the cells the whole biologically active molecule of Ochratoxin A is released into the targeted cells. Fig.7 Synthesis of the toxin ergotamin Toxin ergotamin is cleaved into two precursors, which are bound to oLigomers. After simultaneous hybridization of the oligomer-PBACs to cellular RNA or DNA, the chemically active amino group of precursor "B" interacts with the moiety C(0)-0- which binds precursor "A" w th oligomer "A", to form a chemical bond between the two ergotamin precursors . After degradation of the oligomers, RNA, or DNA in the cells, the whole biologically active molecule of ergotamin is released into the targeted cells. Fig 8. Synthesis of proteins. The synthesis of a biologically active protein of n peptides . Peptides are bound to oligomers simultaneously at their amino and carboxy ends, with the exception of the first peptide, which is bound to the oligomer at its carboxy end, and the last peptide, which is bound to the oligomer at its amino terminal. Two oligomers bound to peptides (oligomer-PAs) are hybridized simultaneously to specific RNA or DNA molecules, the distance from each other between 0 and 10 nucleotides of cellular RNA or DNA. After hybridization, the amino group of the oligomer-PA n interacts with the -L 2-S-C (0) - linking moiety to form a peptidyl bond between peptide "n-1" and peptide "n" . The peptide n _ι is released from the oligomer n -l at its carboxy terminal. The activated -L 2-SH group interacts then with the linking moieties -0-S-L A l and -0-NH-L l which bind peptides n at their amino terminal with oligomers n - After hybridization of all n oligomer- PAs the process is repeated n-1 times to bind all n peptides into one biologically active protein. Linking of the peptides at the amino terminal with oligomers is performed by amino acids which have hydroxyl group such as serine, threonine and tyrosine. Fig 9. Synthesis of proteins. The same process is shown as in figure 8, but this time the peptides are bound at their amino terminal to oligomers through aminoacids with amino and mercapto groups, for example cysteine, arginine, asparagine, glutamine and lysine. The activated -L A 2-SH RECTIFIED SHEET (RULE 91) ISA EP group can interact with the linking groups such as -S-S-IA1, -S- NH-IA1 to form -L A 2-S-S-L i- , -L 2-S-NH-L 1 moieties and to release peptides from oligomers. Fig 10. Synthesis of RNA In this figure "PA n " are oligoribonucleotides comprising from 3 to 303 nucleotides. n in "PA n " means the ordinal number in a series of oligoribonucleotides used in the synthesis of whole RNA, where n is selected from 2 to 1000. PAi couples with PA2 through the chemical moiety -0- , then in turn PAι~m-PA2 couples with PA3 through chemical moiety -0-, then PAι-m-PA2-m-PA3 couples with PA4 through chemical moiety -0- and so on until the last "n"th oligoribonucleotide is bound, forming the whole biologically active RNA. The chemical moieties in figures from 1 to 10 are as follows: m is selected independently from: -S-S-, -N(H)C(0)-, - C(0)N{H)-, -C(S)-0-, -C(S)-S-, -0-, -N=N-, -C(S)-, -C(0)-0-, -NH-, -S-; K A 1 is selected independently from: -NH(2) , dbdNH, -OH, -SH, -F, -CI, -Br, -I, -R A 1-C (X) -X 1-R A 2 ; K A 2 is selected independently from: - NH(2) , -dbd-NH, -OH, -SH, -R A 1-C(X)-X A 1-R A 2, -F, -CI, -Br, -I; L A 1 is independently: chemical bond, -R 1-, -R A l-0-S-R A 2-, -R A l-S-0-R Λ 2-, -R A 1-S-S-R A 2-, -R 1-S-N(H) -R A 2-, -R A 1-N(H)-S-R A 2-, -R A l-0-N(H)-R A 2-, -R 1- (H) -0-R A 2-, -R A 1-C(X)-X A 1-R A 2-; L A 2 is independently: chemical bond, -R Λ 1-, -R A l-0-S-R 2-, -R A l-S-0-R A 2-, -R Λ 1-S-S-R A 2-, -R 1-S-N(H) -R A 2- , -R A 1-N(E)-S-R A 2-, -R A l-0-N(H)-R A 2-, -R A 1-N(H) -0-R A 2- , -R 1-C(X)-X A 1-R A 2-, -R 1-X-C(X)-X-C(X)-X-R 2-; R A 1 is independently: chemical bond, alkyl, alkenyl, alkynyl, aryl, heteroalkyl, heteroalkenyl, heteroalkynyl , heteroaryl, cycloheteroaryl, carbocyclic, heterocyclic ring, X A 1-P(X) (X)-X A 1, -S (O) - , -S(0)(0)-, -X 1-S(X) (X)-X A 1-, - RECTIFIED SHEET (RULE 91) ISA/EP C(O)-, -N(H)-, -N=N-, -X 1-P(X) (X) -X 1-, -X 1-P (X) (X) -X A 1- P(X) (X) -X 1, -X A 1-P(X) (X) -X 1-P(X) (X)-X A 1-P(X) (X)-X 1, C(S)-, any suitable linking group; R A 2 is independently chemical bond, alkyl, alkenyl, alkynyl, aryl, heteroalkyl, heteroalkenyl , heteroalkynyl , heteroaryl, cycloheteroaryl, carbocyclic, heterocyclic ring, X A 1- P(X)(X)-X A 1, -S(O)-, -S(0)(0)-, -X 1-S(X) (X)-X A 1-, -C(0)-, - N(H)-, -N=N-, -X 1-P(X) (X)-X A 1-, -X 1-P (X) (X) -X A 1-P (X) (X) - X A 1, -X A 1-P(X) (X)-X 1-P(X) (X)-X A 1-P(X) (X)-X 1, -C(S)-, any suitable linking group; X is independently S, 0, NK, Se, alkyl, alkenyl, alkynyl; X 1 is independently S, 0, NH, Se, alkyl, alkenyl, alkynyl. Best mode for carrying out the invention. The synthesis of different toxins and alkaloids directly into targeted cells. Example 1. The synthesis of the toxin alpha amanitin. The amanitin is a toxin present in mushrooms. It acts as a very strong inhibitor of transcription in eucaryotic cells, and is therefore very strong toxin. The synthesis of alpha-amanitin is represented in Fig.4 The structure of the toxin is a cyclic peptide with modified amino acids. The molecule of alpha-amanitin can be cleaved into two inactive precursors, which are bound to 4 oligomers through linking moieties L A 1 and L A 2, designated in Figure 4. After hybridization of all oligomers to the same molecule of RNA the synthesis of toxin amanitin is occurred. Example 2. The synthesis of biologically active peptides. The synthesis of BACs consisting of amino acids makes possible the synthesis of practically any peptide. These peptides car. be involved in a wide variety of processes. The specific synthesis will occur only in the cells where the specific sequences are represented. The synthesis of peptides such as endorphins or toxins which block Na, K, Ca channels can be performed directly on specific RNA or DNA sequences. These peptides can act as agents RECTIFIED SHEET (RULE 91) ISA/EP stimulating cells of the nervous system, or as analgesic agents. To date, the number of known biologically active peptides is enormous . The peptides can be synthesized from natural amino acids as well as from synthetic amino acids of D or L conformations. The synthesis of neurotoxin is represented in Fig.2. Example 3. The synthesis of the toxin tulopsoid A. Toxin t-ilopsoid A is an alkaloid and is a strong cytolitical toxin. Toxin tulopsoid A is cleaved into two precursors. The chemically active hydroxyl group of precursor "A" can interact after hybridization with the -CH2-S-C(0)- moiety to form a chemical bond with tulopsoid' s precursor "B", with the release of precursor "B" from the oligomer. The activated -CH2-SH moiety interacts with the linking moiety -S-0-, releasing the whole tulopsoid from oligomer (Fig. 3.). Example 4. The synthesis of the toxin daphnoretin. Toxin daphnoretin is an alkaloid and is a strong cytolitical toxin. Toxin Daphnoretin is cleaved into two precursors. After simultaneous hybridization of the oligomers coupled to the daphnoretin ' s precursors the chemically active hydroxyl group of daphnoretin ' 3 precursor "A" interacts with the chemically active Cl group oE precursor "B" to form chemically bond between daphnoretin ' 3 precursors . The degradation of the oligomers or linking groups leads to the release of the biologically active molecule directly into targeted cells (Fig.4) . Example 5. The synthesis of the toxin D-actinomicin. Toxin D-actinomicin is an alkaloid and is a strong cytolitical toxin. Toxin D-actinomicin is cleaved into two precursors. After hybridization of two oligomers to cellular RNA or DNA, the chemically active groups amino and halogen of precursor "A" interact with the chemically active groups halogen and hydroxyl respectively of D-actinomicin ' s precursor "B" to form two chemical bonds between the precursors (Fig 5.). Example 6. The synthesis of the toxin ochratoxin A. Toxin ochratoxin A is an alkaloid and is a strong cytolitical toxin. Toxin ochratoxin A is cleaved into two precursors bound to oligomers. After hybridization of the oligomers to cellular RNA RECTIFIED SHEET (RULE 91) ISA/EP or DNA, the chemically active amino group of the precursor "B" interacts with the moiety -O-C(O) of precursor "A" to form a chemical bond between the two ochratoxin precursors. After the degradation of the oligomers or linking moieties in the cells, whole, biologically active molecules of Ochratoxin A will be released into targeted cells (Fig. 6.). Example 7. The synthesis of the toxin ergotamin Toxin ergotamin is an alkaloid and is a strong cytolitical toxin. Toxin ergotamin is cleaved into two precursors which are bound to oligomers. After hybridization of the oligomers to cellular RNA or DNA, the chemically active amino group of precursor "B" interacts with moiety -O-C(O) of precursor "A" to form a chemical bond between the two ergotamin precursors. After degradation of the oligomers or linking moieties in the cells, whole, biologically active molecules of ergotamin will be released into the targeted cells . By using more than two oligonucieotides bound at their 5* ,3' ends to precursors of biologically active compounds, higher concentration level of the biologically active substances can be achieved into targeted cells . A B A B on OI2 OI3 -r_-ι J V_ -z-r \ on / i ol2 / \ ° 13 Oil, 012, 013 are oligomers 1,2,3 which at their 3' and 5' ends are bound to precursors of biologically active substances . Such linking can also prevent oligonucieotides from exonuclease degradation and stabilise their activity in cells. In any case, the products of the degradation of the peptides and oligonucieotides formed from natural amino acids and nucleotides are not toxic, and can be used by cells without elimination from the organism or toxic effects on other healthy cells. RECTIFIED SHEET (RULE 91) ISA/EP All the toxins described can be used for the synthesis of toxins in cells infected by viruses, using the hybridization of the oligomers to double stranded DNA. In USA patent 5,571,937 the homopurine sequences of HIV 1 were found. One such sequence is 5 ' -GAAGGAATAGAAGAAGAAGGTGGAGAGAGAGA-3 ' (seq ID NO 43 USA patent 5,571,937). Using two oligomers: (A-5'- GAAGGAATAGAAGAAG-3 ' ) and (B-5 ' -AAGAAGGTGGAGAGAGAGA-3 ' ) bound through linking moieties IA1 and L 2 to PBACs, synthesis of the corresponding BACs directly in human cells infected by HIV1 can be achieved. The toxin will be synthesized only in those cells infected by HIV1. Other healthy cells will be not killed by synthesized toxin. The synthesis of proteins The synthesis of protein can be performed according to the scheme designated in Formulas 5, 6 and 7 and in Figs .8 , 9. Relatively small molecules can be used to synthesise the whole active proteins in any tissue of a living organism. These small molecules can easily penetrate the blood brain barrier, or enter other tissues. The degradation products of such compounds can be used as nutrients for other cells. They are also not toxic to other cells where specific RNAs are not present, in the case where oligomers are oligoribo (deoxy) nucleotides . The synthesis of whole proteins of 50 kDa can be performed on one template 300-500 nucleotides in length using oligomers of the length 10-50 nucleomonomers bound to peptides consisting of 2-30 amino acids. Only 10-20 such PBACs are necessary to synthesise a protein of molecular weight 50 kDa. Theoretically, it is possible to synthesise the proteins of any molecular mass. The number of oligomer-PAs can vary from 1 to 1000, but the efficiency of synthesis of large proteins is very low and depends on the velocity of the reaction and the degradation of the oligomer-PAs in the living cells . By this method, synthesized proteins can be modified later in the cells by cellular enzymes to achieve the biologically active form of the protein. The method allows the synthesis of specific proteins only in those cells in which the proteins are needed. Any type of proteins can be synthesized by this method. These proteins can be involved in cellular metabolism, transcription regulation, RECTIFIED SHEET (RULE 91) ISA/EP enzymatic reactions, translation regulation, cells division or apoptosis . The mechanism allows the synthesis of any protein directly into targeted cells. The synthesized proteins could inhibit a cell's growth or division, or could stimulate division and metabolism of cells where specific RNAs are expressed. By the method described, it is possible to synthesise not only one protein, but also many different proteins in the selected cells. These proteins could change even the differentiation of the targeted cells. The targeted cells can be somatic cells of living organisms, tumour cells, cells of different tissues, bacterial cells or cells infected by viruses. Example 8 Synthesis of the tumour suppresser p53. The synthesis is performed according to Formula 6. In the example below, the peptides from PA2 to PA14 are bound at their NH2 end to the linking moiety L A 2 through the OH group of amino acids serine or threoninε. The linking moiety L 2 is bound to the phosphate or sugar moiety of the nucleotides localised at the 5' end of the corresponding oligomers. The amino acids at the COOH ends of the peptides are bound to the oligomer through acyl moieties (L A 1) bound to the 3' OH group of sugar moiety of the nucleotide localised at 3 ' end. After hybridization to specific cellular RNA, the NH2 group of the oligomer n -PA n interacts with the linking acyl group of the oligomer n -ι-PA n _ι to form a peptidyl bond between two oligomer-PAs. The whole P53 protein can be synthesized using only 14 oligomer-PAs and a 250 nucleotide long region of RNA for hybridization to the oligomer-PAs. PAl, PA2, P 3, PA4, PA5, PA6, PA7 , PA8 , PA9 , A10, PAn, PA12, PA13 and PA14 are the peptides which are bound to the oligomers. Tne sequences of the peptides are represented below. PAi - MEEPQSDPSV EPPLSQETFS DL WKLLPENN VL A2 -SPLPSQAM DDLMLSPDD! EQWF PA3 -TEDPGPDEAP RMPEAAPRVA PAPAAP PA4.TPAAPAPAPS WPLSSSVPSQ KTYQG PA 5 -SYGFRLGFLHS GTAKSVTCTY PA6 -SPAL NKMFCQLAKT CPVQLWVDSTPPPG PA7- TRVRAM AIYKQSQHMT EVVRRCPHHE PAs - TCSDSDGLAP PQHLIRVEGN LRVEYLDDRN RECTIFIED SHEET (RULE 91) ISA/EP PAg - TFRHSVVVPY EPPEVGSDCT TIHYNYMCNS PA10 - SCMGGMNRRP ILTIITLEDS SGNLLGRN PA 1 -SFEVRVCACPGR DRRTEEENLR KKGEPHHELPPG PA12 -STKRALPN NTSSSPQPKK KPLDGEYF PΔi3 - TLQIRGRERFEM FRELNEALEL KDAQAGKEPGG PA14.SRAHSSHLK SKKGQSTSRH KKLMFKTEGP DSD Amino acids are designated in bold/italicised one letter code. A - alanine, R - arginine, N - asparagine, D - aspartic acid, C- cysteine, Q -glutamine, E - glutamic acids, G - glycine, S - histidine, I - isoleucine, 1 -leucine, K - lysine, M - methionine, F - phenylalanine, P- proline, S - serine, T - threonine, W- tryptophan, Y - tyrosine, V - valine. The tyrosine in PA7 can be chemically phosphorylated. In this way an already active form of the protein can be synthesized directly in the cells . It is possible to include any modification at any amino acid of the PAs. oligomer 1 5 ' -cccaatccctcttgcaactga-3 ' oligomer 2 5 ' - attctactacaagtctgccctt-3 ' oligomer 3 5 ' -ttgtgaccggctccactg-3 ' oligomer 4 5 ' -taccttggtacttctctaa-3 ' oligomer 5 5 ' -atgccatattagcccatcaga-3 ' oligomer 6 5 ' -ccaagcattctgtccctccttt-3 ' oligomer 7 5 ' -tccggtccggagcacca-3 ' oligomer 8 5 ' -gccatgacctgtatgttaca-3 ' oligomer 9 5 ' -ggtgtgggaaagttagcggg-3 ' oligomer 10 5 ' -gcgaattccaaatgattttaa-3 ' oligomer 11 5 ' -aatgtgaacatgaataa-3 ' oligomer 12 5 ' -agagtgggatacagcatctata-3 ' oligomer 13 5 ' -acaaaaccattccactctgatt-3 ' oligomer 14 5 ' -ttggaaaaactgtgaaaaa-3 ' All oligomers herein are oligonucieotides antiparallel to the human plasminogen antigen activator mRNA. After hybridization of the oligomer-PAs to the RNA, the distance between the 3 ' ends of the oligomer n -l and the 5 ' ends of the oligomer n is equal to 0 nucleotides of plasminogen antigen activator mRNA. n as used herein is from 1 to 14. 2N-MEEPQSDPSVEPPLSQETFSDLWKLLPENNVL RECTIFIED SHEET (RULE 91) ISA/EP Oligomerl-PAi is L Λ 1 5 -cccaatccctcttgcaactga-3' H2U-SPLPSQAMDDLMLSPDDIEQWF 01igomer2 ~PA2 is 1_Λ 2 |_ Λ 1 5 - attctactacaagtctgccctt - 3' H2 - TEDPGPDEAPRMPEAAPRVAPAPAAP Oligomer3 ~PA3 is L Λ 2 L Λ 1 5'-ttgtgaccggctccactg -3' H 2 N- TPAAPAPAPSWPLSSSVPSQKTYQG Oligomer4 -PA4 is L Λ 2 L Λ 1 5'-taccttggtacttctctaa -3' H 2 N SYGFRLGFLHSGTAKSVTCTY Oligomer5 -PA5 is I_Λ 2 |_ Λ 1 5'-atgccatattagcccatcaga -3' H 2 N-SPALNKMFCQLAKTCPVQLWVDSTPPPG Oligomerδ-PAβ is L Λ 2 L Λ 1 5'- ccaagcattctgtccctccttt-3' H 2 N- TRVRAMAIYKQSQHMTEVVRRCPHHE Oligomer7 -PA7 is L Λ 2 L Λ 1 5'- tccggtccggagcacca-3' H2N- TCSDSDGLA PPQHLIR VEGNLRVEYLDDRN Oligomer8 -PA8 is L Λ 2 L Λ 1 5'-gccatgacctgtatgttaca - 3 1 H2M-TFRHSVWPYEPPEVGSDCTTIHYNYMCN Oligomer9 -PAg is Λ 2 Λ 1 5'- ggtgtgggaaagttagcggg-3' 2hi-sscMGGMNRRPiLτιιτLEDssGNLLGRN OligomeriQ -PAio is L Λ 2 L Λ 1 5'- gcgaattccaaatgattttaa-3 1 2 SFEVRVCACPGRDRRTEEENLRKKGEPHHELPPG Oligomern -PAn is L Λ 2 L Λ 1 5'- aatgtgaacatgaataa-3' H 2 H-sτκRALPNNτsssPQPκκκPLDGEYF Oligomeri2 -PAi2 is L Λ 2 L Λ 1 5'- agagtgggatacagcatctata-3 1 H∑N-TLQIRGRERFEMFRELNEALELKDAQAGKEPGG 01igomeri3 -PAi3 is L Λ 2 L Λ 1 5'-acaaaaccattccactctgat-t-3' H2NSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD 01igomeri4 -PAi4 is L Λ 2 5'-ttggaaaaactgtgaaaaa-3' The oligomer n -PA n (n is selected from 1 to 14) are peptides chemically bound to oligomers which can form stable duplex structure with the plasminogen antigen activator mRNA expressed in human ovarian tumour cells . Using the plasminogen antigen activator mRNA it is possible to synthesize any other protein or small BAC. All these proteins or BACs will be synthesized only in those cells where the human plasminogen activator mRNA is expressed. In the case of the human plasminogen activator mRNA, the synthesis of the protein cr BAC will occur only in ovarian tumour cells. Oligomer 1 at its 3' end is bound to the "C" end of the peptide PAi of p53 through the linking moiety L 1. Oligomers 2 to 13 are bound at their 5 ' and 3 ' ends to peptides A2 to PA13 at their "N" and "C" ends respectively, through the linking moieties L A 2 and L 1. 01igomerl4 at it's 5' end is bound to the "N" end of the peptide PA14 of p53 through the linking moiety L A 2. The first methionine of PAi is formylated, and the amino end of peptidei is not bound to Oligomerl . The last amino acid at the carboxyl end of PA1.4 is not bound to 01igomeri4. Only 14 peptides chemically bound to 14 oligomers are required to synthesize p53 tumour suppresser specifically in the cells of the ovarian tumour. In any type of tumour cell RNAs specific to this cell type are expressed. By this method, it is possible to synthesise any protein or BACs described above on these RNAs . The 14 Oligomer-PAs are hybridized on the mRNA in such a manner that the 3' end of the oligomeri-PAi is located at a distance from the 5' end of the oligomer2-PA2 which is equal to 0 nucleotides of the plasminogen antigen activator mRNA. The distance between the 5' end of the Oligomer3-PA3 and the 3' end of the Oligomer2-PA2 is equal to 0 nucleotides of the plasminogen antigen activator mRNA. The distance between the 5' end of the Oligomer4-PA4 and the 3' end of the oligomer3-PA3 is equal to 0 nucleotides of the plasminoger- antigen activator mRNA etc. In other words . after hybridization of the oligomer-PAs to the plasminogen antigen activator mRNA, the distance between the 3 ' end of the oligomer n -i-PA n -l and the 5' end of the 01igomer n -PA n is equal to 0 nucleotides cf the plasminogen antigen activator mRNA. After the degradation of the oligomers and/or linking moieties, the synthesized protein p53 is released into the determined cells. {H2N-PA1-C (0)NH-PA2-C (0)NH-PA3-C (0)NH-PA4~C (0)NH-PA5-C (0)NH-PA6~ -C(0)NH-PA7- (0)NH-PA8-C(0)NH-PAg-C(0)NH-PAio-C(0)NH-PAii-C(0)NH- -PAi2-C(0)NH-PAi3-C(0)NH-PAi4-C00H) is biologically active protein - tumour suppresser p53. The yield of synthesis in the cells can bo very low, even <1%, because .the synthesis occurs directly into the targeted cells. Using different RNAs transcribed at different levels in the same cells, it is possible change the amount of the protein synthesized by this method. The variety of proteins , which can be synthesized by the proposed method, is enormous. Limitations could occur if the proteins to be synthesised are very large or have many hydrophobic amino acids. The distance between the 5 ' and 3 ' ends of the oligomer-PAs after hybridization to the template can be varied between 0 and 10 nucleotides of the target RNA. In the; example described above, the oligomers are antiparallel to the plasminogen antigen activator mRNA. Using RNAs which expressed specifically in different tumour cells, the synthesis of any protein in these cells can be achieved. One example of such RNA is metastasin (mts-1) mRNA (Tulchinsky et al.1992, accession number g486654) . Using oligomers antiparallel to metastasin mRNA it is possible to synthesise any toxin or protein specifically in human metastatic cells . Using different RNAs expressed specifically in different tissues or in cells infected by viruses, or in bacterial cells, it is possible to synthesise any toxin or protein specifically in these cells. The example 10 Synthesis of the tumour suppresser p53 according to Formula 7. After hybridization of the oligomer-PAs to mRNA specific to RECTIFIED SHEET (RULE 91) 'SA'EP ovarian tumour cells (NbHOT Homo sapiens mRNA accession number AA402345) , the chemical moiety K 1 of PA 2 (in this example K 1 is NH2 group) interacts with the linking moiety L Λ 2 of the oligomer -PAi . After the interaction has occurred, the peptide PAi is bound through the peptidyl bond to the peptide PA2 and is released from the 5' end of the oligomeri. The linking moiety L 2 of the oligomeri is activated so that it interacts with the linking moiety LAI of oligomer 2 , and the peptide PA 1 -C(0)NH-PA2 is released from the 3 ' end of oligomer 2 . The chemical moiety K 1 of oligomer3 -PA3 interacts with the linking moiety L A 2 of oligomer2-{PA 1 -C(0)NH-PA2) to bind peptide PA3 with PA 1 -C(0)NH- A2 , releasing peptide PA 1 -C (0)NH-PA2-C(0)NH-PA3 from oligomer2. The activated linking moiety IA2 of oligomer 2 interacts with the linking moiety IA1 and releases the peptide PA-^-c (0)NH-PA2~C (O)NH- PA3 from the 3 ' ends of oligomer3. The processes described above are repeated in the cells 13 times. In such as manner, the protein: {PA- | _-C (0)NH-PA2-C (0)IIH-PA3-C (0)NH-PA 4 ~C (ONH-PA5-C (O)NH- PA6-C(O)NH-PA 7 -C(O)NH-PA8-C(O)NH-PA9-C(O)NH-PA 10 -C(O)NH-PAn- C(0)NH-PAi2-C(0)NH-PA 13 -C(0)NH-PAi4} can be synthesized. Neither the degradation of the oligomers nor the degradation of the linking moieties is necessary to release the protein from the oligomers. Peptidyl bond formation between Pa n -1 and PA n and degradation of the linking moieties L A 2 proceed simultaneously with the release of PAs from the 5' ends of the oligomers. The activated linking moieties L 2 interact with the linking moieties L 1 to release the bound peptides from the 3' ends of the oligomers . PAi -MEEPQSDPSVEPPLSQETFSDLWKLLPENNVL PA2 -SPLPSQAMDDLMLSPDDIEQWF PA 3 - TEDPGPDEAPRMPEAAPRVAPAPAAP PA4 -TPAAPAPAPSWPLSSSVPSQKTYQG PA 5 -SYGFRLGFLHSGTAKSVTCTY PA6 -SPALNKMFCQLAKTCPVQL WVDSTPPPG PA7- TRVRAMAIYKQSQHMTEVVRRCPHHE PAs - TCSDSDGLAPPQHLIRVEGNLRVEYLDDRN PA9 - TFRHSVVVPYEPPEVGSDCTTIHYNYMCNS PA10 - SCMGGMNRRP1LTUTLEDSSGNLLGRN PAn -SFEVRVCACPGRDRRTEEENLRKKGEPHHELPPG PAi 2 -STKRALPNNTSSSPQPKKKPLDGEYF PAi 3 -TLQIRGRERFEMFRELNEALELKDAQAGKEPGG PAi 4 .SRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD where PAi to PA14 are peptides bound to oligomers , Amino acids are designated in bold/ italicised one letter code . A - alanine , R - arginine , N - asparagine , D - aspartic acid, C- cysteine , Q -glutamine , E - glutamic acids , G - glycine , H - histidina , J -isoleucine , L-leucine , K - lysine , M - methionine , F - phenyl alanine , P- proline , S - serine , T - threonine, W- tryptophan, Y - tyrosine , V - valine . Oligomeri 3' ATGGGCGGTAGGTAC 5" Oligomer2 3' TAGCGGTGCCCTCGA 5' Oligomer3 3' AACCCCGACGTCACG 5' Oligo er4 3' TTCCGGACCCACGGA 5' Oligomers 3' CGAGGTACAGGCCCC 5 1 Oligomer6 3' TACTCGAGTGTCTCG 5' Oϋgomei7 3' ACGACCGTCCCTAGT 5' Oligomers 3" GACCGTGACTTCACC 5' Oligomerg 3' TGACGGACGCCCGGA 5' Oligomeri 0 3" CAGTCCTCGTCTAGC 5' Oligomeri 1 3' TTCGACGTGAGTCCC 5' Oligomeπ 2 3' TCTCGGAGTCCCTTC 5' Oligomeri 3 3' GGAGAGTCTGGTCGA 5' Oligomern 3' GGTCGGGTCGCGGGT 5' Oligomers are complementary ( antiparallel) to NbHOT Homo sapiens mRNA (clone 741045 accession number AA402345 ) which is specific to ovarian tumour cells . The distance of the oligomers each from other is null nucleotides of the NbHOT Homo sapiens mRNA . MEEPQSDPSVEPPLS Q ETFSDLWKLLPENNVL Oligomer -PAi is L Λ 2 3' ATGGGCGGTAGGTAC 5' (K^)SPLPSQAMDDLMLSPDDIEQWF RECTIFIED SHEET (RULE 91) ISA/EP Oligomer2-PA 2 is L Λ 1 L Λ 2 3' TAGCGGTGCCCTCGA 5' (K Λ 1 )TEDPGPDEAPRMPEAAPRVAPAPAAP Oligomer3-PA3 is L Λ 1 L Λ 2 3' AACCCCGACGTCACG 5' (K^ ) TPAAPAPAPSWPLSSSVPSQKTYQG Oligomer4-PA4 is L Λ 1 L Λ 2 3' TTCCGGACCCACGGA 5' (K^)SYGFRLGFLHSGTAKSVTCTY Oligomer5-PA5 is L Λ 1 L Λ 2 3' CGAGGTACAGGCCCC 5' (K^ )SPALNKMFCQLAKTCPVQL WVDSTPPPG Oligomer6-PA6 is L Λ 1 L Λ 2 3' TACTCGAGTGTCTCG 5' (K^ )TRVRAMAIYKQSQHMTEVVRRCPHHE Oligomer7-PA7 is L Λ 1 L Λ 2 3' ACGACCGTCCCTAGT 5' (K -\ )TCSDSDGLAPPQHLIRVEGNLRVEYLDDRN Oligomer8-PA8 is L Λ 1 L Λ 2 3' GACCGTGACTTCACC 5' ( K Λ 1 ) TFRHS VVVP YEPPE VGSDCTTIH YN YMCNS Oligomerg-PAg is L Λ 1 L Λ 2 3' TGACGGACGCCCGGA 5' (K^ )SCMGGMNRRPILTIITLEDSSGNLLGRNS Oligomeri 0-PA 0 is L Λ 1 L Λ 2 3 * CAGTCCTCGTCTAGC 5' (K^ )FEVRVCACPGRDRRTEEENLRKKGEPHHELPPGS Oligomeri 1 -PA 1 is L Λ 1 L Λ 2 3' TTCGACGTGAGTCCC 5' (κ^)TKRALPNNTSSSPQPKKKPLDGEYF Oligomeri 2 -PAι is Λ 1 L Λ 2 3' TCTCGGAGTCCCTTC5' (K^ )TLQIRGRERFEMFRELNEALELKDAQAGKEPGG Oligomeri 3-PA13 is Λ 1 L Λ 2 3' GGAGAGTCTGGTCGA5' (W\)SRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD Oligomeri 4-PA14 is L Λ 1 3' GGTCGGGTCGCGGGT 5' This method of protein synthesis also allows modification of the synthesized protein. Certain amino acids of the peptides used in the synthesis can be glycosylated or phosphorylated. Glycosylation of a protein is a complex process, and difficulties may occur in the penetrance of some tissues with the glycosylated form of the peptide due to the size of the molecule. However the use of phosphorylated peptides opens up the possibility to synthesize already active proteins in the cells of living organisms. The synthesis of RNA. Using the method described above, it is possible to synthesise into targeted cells not only proteins but also RNAs. An example of such synthesis is represented in Fig.10 To synthesize whole RNA in cells from n oligomers bound to oligoribonucleotides (oligomer-PAs) the concentration of such oligomer-PAs must be high. After the simultaneous hybridization of oligomer-PAs to the same molecule of the cellular RNA, the chemically active 3 ' hydroxyl group of the oligoribonucleotide PAi interacts with the linking moiety -L A 2-S- which bound oligoribonucleotide A2 with oligomer 2. In this case the linking group is represented with an -S-L A 2- moiety which is coupled to phosphate group of the oligoribonucleotide PA2. The 3 ' hydroxyl group of the oligoribonucleotide PAi interacts with the linking group of PA2 forming a chemical bond with the phosphate group, releasing the oligoribonucleotide PA2 at it's 5' end from oligomer 2, and activating the linking moiety with the formation of the -SH group. This chemically active group -SH RECTIFIED SHEET (RULE 91) ISA/EP interacts with linking moiety -IA1-S which couples the oligomers. This process is repeated n-1 times to bind all PAs in one molecule. PAi is bound through chemical moiety -0- to A2 , then in turn PAι-m-PA2 is bound through chemical moiety -0- to PA3 , then PAι-m-PA2-m-PA3 is bound through chemical moiety -0- to PA4 and so on until the last oligcribonucleotide is bound, forming whole biologically active RNA. In this figure "PA n" are oligoribonucleotides comprising from 3 to 300 nucleotides. n in "PA n " means the ordinal number in a series of oligoribonucleotides used in the synthesis of a whole RNA, where n is selected from 2 to 1000.

Claims: Claims : 1. A process for synthesis of biologically active compounds (BACs) from biologically inactive BAC precursors (PBACs) "A", "B" and "PA n " chemically bound to 5' and/or 3' ends of the oligomers directly in cells of living organisms according to Formulas 1 to 7 , which process comprises: (a) at least two oligomers, chemically bound at their 5' and/or 3 ' ends to biologically inactive precursors of the biologically active compounds (oligomer-PBACs) , are hybridised simultaneously to cellular RNA, DNA or dsDNA in vivo in cells of a living organism, so that after hybridization the distance between the 5' or 3' ends of the oligomer-PBAC "A" and the 3' or 5 ' ends of the oligomer- PBAC "B" is from 0 to 8 ribo (deoxy) nucleotides of cellular RNA, DNA or dsDNA correspondingly, and the chemically active groups K 2 and K 1 of the biologically inactive PBACs "A" and "B" can interact with each other or with linking moieties L A 1 and L 2 to form chemical moiety "m" between PBAC "A" and PBAC "B" so that "A"-m-"B" is equal to the biologically active compound "T"; (b) (Formula 1) the same process as in (a) , but after hybridization of the "oligomer-PBACs" "A" and "B" to cellular RNA, DNA or dsDNA, the chemically active groups K 1 and 2 of the oligomer-PBACs "A" and "B" interact with each other to form the chemical moiety "m" , which combines PBACs "A" and "B " into one active molecule of the biologically active compound "T" , the degradation of the oligomers and/or linking moieties L A 1 and L 2 by cellular enzymes or hydrolysis leads to the release of the synthesized BAC "T" directly into targeted cells of a living organism; (c) (Formula 2) the same process as in (a), but after hybridization of "oligomer-PBACs" "A" and "B" to cellular RNA, DNA or dsDNA, the chemically active group K A 2 of oligomer-PBAC "B" interacts with the linking moiety L A 1 of oligomer-PBAC "A" to combine the PBACs through chemical moiety "m" , into one active molecule of the biologically active compound "T", releasing the PBAC "B" from the oligomer and the oligomer "A" and/or linking moieties L A 1 are degraded by cellular enzymes or hydrolysis leading to the release of the synthesized BAC "T" directly into targeted cells of a living organism; (d) (Formula 3) the same process as in (a), but after hybridization of "oligomer-PBACs" "A" and "B" to cellular RNA, DNA or dsDNA, the chemically active group K A 1 of the oligomer-PBACs interacts with the linking moiety L 2 to combine the PBACs through chemical moiety "m" into one active molecule of the biologically active compound "T", releasing the PBAC "B" from the oligomer and activating the chemical moiety L 2, which after activation interacts with the linking moiety L A 1 to release the biologically active compound "T" from oligomer directly into targeted cells of a living organism. (e) (Formula 4) the same process as in (a) , but after hybridization of "oligomer-PBACs" "A" and "B" to cellular RNA, DNA or dsDNA, the chemically active group K A 2 of oligomer-PBAC "B" interacts with the linking moiety L A 1 of the oligomer-PBAC "A" to combine the PBACs through the chemical moiety "m" , and the chemically active group K 1 of the oligomer-PBAC "A" interacts with the linking moiety L A 2 of the oligomer-PBAC "B" to form chemical moiety m A l which, together with the chemical moiety m, combines two "PBACs" into one active molecule of the biologically active compound "T", with the release of the PBAC "B" from the oligomer. 2.The process of claim 1 but: (a) the synthesis of the BAC "PR" in the cells of living organisms is performed from n "oligomer n -PA n "s so that "oligomer n -l-PA n -l" and "oligomer n -PA n " are hybridized simultaneously on the same molecule of cellular RNA, DNA or dsDNA, with a distance of from null to eight nucleotides of cellular RNA or DNA between the 3 ' or 5 ' ends of the oligomer n _ι-"PA n -l" , and the 5' or 3 ' ends of the oligomer n - "PA n " correspondingly, here n is selected from 2 to 2000; (b) (Formula 5) the same process as in (a), but after simultaneous hybridization of "oligomer n -l-PA n -l " and " oligomer n -PA n " to cellular RNA or DNA, the chemically active groups K 1 and K 2 interact with each other to form the chemical moiety " " between " oligomer n _ι-PA n -i » and "oligomer n -PA n" correspondingly, this step is repeated in the cells n-1 times and combines n-1 times all "PA n "s into one active molecule of biologically active compound "PR" which consists of n PA n sc that the compound { "PA"ι-m-"PA"2- m-"PA"3-m-"PA"4-m- ... -m- "PA n _3 • ■ -m- "PA n -2 " -m- "PA n -l " -m- "PA n " } is the biologically active compound "PR"; the degradation of the oligomers and/or linking moieties IA1 and L 2 leads to the release of synthesized BAC "PR" directly in the targeted cells of a living organism, here n is selected from 2 to 2000; (c) (Formula 6) the same process as in (a), but after simultaneous hybridization of "oligomer n -l-PA n -l " and "oligomer n -PA n " to cellular RNA, DNA or dsDNA chemically active group K A 1 of "oligomern-l-PAn-l" interacts with the linking moiety L A 2 of "oligomer n -PA n " to bind PA n _ι and PA n through the chemical moiety "m" , this step is repeated in the cells n-1 times, and combines n-1 times all PA n s after hybridization of all n "01igomer n -PA n" s into one active molecule of biologically active compound "PR", which consists of n PA n so that the compound {PAι-m-PA2-m-PA3-m- PA4-1T.- ... -m-PA n -3- -PAn-2-r n - A n-l~ m -" PA n} is equal to the biologically active compound PR; the degradation of the oligomers and/or linking moieties IA1 and L 2 due to cellular enzymes or hydrolysis leads to the release of the synthesized BAC "PR" directly into targeted cells of a living organism, here n is selected from 2 to 2000; (d) (Formula 7) the same process as in (c), but after interaction of K A 1 with L A 2 , L 2 is chemically activated so that it can interact with the linking moiety L 1 of oligomer-PAn-i, destroying the binding of oligomer n -l with PA n _ι, this step is repeated n-1 times, so that only whole BAC "PR" consisting of n PA n s {PAι-m-PA2-m-PA3-m-PA4-m- ... - -PA n -3-m-PAn-2- m - pA n-i- m - pA n} is released directly into targeted cells of a living organism, here n is selected from 2 to 2000. 3. In claims 1 and 2 the linking moieties L 1 and L 2 are bound to the first and/or last mononucleomers of the oligomers at their sugar or phosphate moiety, or directly to base, or to sugar moiety analogues, or to phosphate moiety analogues, or to base analogues . 4. In claim 1, biologically inactive precursors of BAC "A" and "B" are selected from chemical substances which can be bound to each other through the chemical moiety "m" , so that the compound A-m-B is the biologically active compound "T" : A-O-B is equal to a whole BAC "T" A-NH-C(0)-B is equal to a whole BAC "T" A-C(0)-NH-B is equal to a whole BAC "T" A-C(0)-B is equal to a whole BAC "T" A-C(S)-B is equal to a whole BAC "T" A-NH-B is equal to a whole BAC "T" A-dbdN—B is equal to a whole BAC "T" A-C(0)0-B is equal to a whole BAC "T" A-C(0)S-B is equal to a whole BAC "T n A-C(S)S-B is equal to a whole BAC "T" A-S-S-B is equal to a whole BAC "T" A-C(S)0-B is equal to a whole BAC "T" A-N=N-B is equal to a whole BAC "T" 5. In claim 2, biologically inactive precursors of BAC PA n are selected from biologically inactive peptides and oligoribonucleotides so that the compound { "PAi"-m-"PA2"-m-"PA3"-m- ... -m- "PA n -2 " -m- "PA n _ι " -m- "PA n " } is equal to the biologically active compound "PR", which is a protein or a RNA. 6. Chemical moieties in claims 1, 2,3 and 4 are as follows: m is selected independently from: -S-S-, -N(H)C(0)-, - C(0)N(H)-, -C(S)-0-, -C(S)-S-, -0-, -N=N-, -C(S)-, -C(0)-0-, -NH-, -S-; K A 1 is selected independently from: -NH(2), dbdNH, -OH, -SH, -F, -CI, -Br, -I, -?. 1-C(X)-X A 1-R A 2; K A 2 is selected independently from: - NH(2), -dbd-NH, -OH, -SH, -R 1-C(X)-X A 1-R 2, -F, -CI, -Br, -I; L A 1 is independently: chemical bond, -R A 1-, -R l-0-S-R A 2-, -R l-S-0-R A 2-, -R A 1-S-S-R 2-, -R A 1-S-N(H) -R 2- , -R A 1-N(H)-S-R 2-, -R A l-0-N(H)-R 2-, -R 1-N(H) -0-R 2-, -R A 1-C(X)-X-R A 2-; L 2 is independently: chemical bond, -R 1-, -R A l-0-S-R A 2-, -R A 1-S-C-R A 2-, -R 1-S-S-R 2-, -R 1-S-N(H) -R A 2- , -R A 1-N(H)-S-R A 2-, -R A l-0-N(H)-R A 2-, -R 1-N(H) -0-R A 2-, -R A 1-C(X)-X A 1-R A 2-, -R A 1-X-C(X)-X-C(X)-X-R A 2-; R 1 is independently: chemical bond, alkyl, alkenyl, alkynyl, aryl, heteroalkyl, heteroalkenyl, heteroalkynyl , heteroaryl, cycloheteroaryl, carbocyclic, heterocyclic ring, X A 1-P(X) (X)-X A 1, -S(O)-, -S(0)(0)-, -X A 1-S(X) (X)-X A 1-, - C(O)-, -N(H)-, -N=N-, -X A 1-P(X) (X)-X 1-, -X A 1-P (X) (X) -X A 1- P(X)(X)-X A 1, -X 1-P(X) (X)-X A 1-P(X) (X)-X A 1-P(X) (X)-X A 1, - C(S)-, any suitable linking group; R A 2 is independently chemical bond, alkyl, alkenyl, alkynyl, aryl, heteroalkyl, heteroalkenyl, heteroalkynyl, heteroaryl, cycloheteroaryl, carbocyclic, heterocyclic ring, X 1- P(X)(X)-X 1, -S(0)-, -S(0)(0)-, -X A 1-S(X) (X)-X A 1-, -C(0)-, - N(H)-, -N=N-, -X A 1-P(X) (X)-X A 1-, -X A 1-P (X) (X) -X 1-P (X) (X) - X A 1, -X A 1-P(X) (X)-X A 1-P(X) (X)-X A 1-P(X) (X)-X 1, -C(S)-, any suitable linking group; X is independently S, 0, NH, Se, alkyl, alkenyl, alkynyl; X A 1 is independently S, 0, NH, Se, alkyl, alkenyl, alkynyl. 7. Biologically active compound "T" which can be synthesized according the processes presented in claims 1 and 3 include but are not limited to: a) biologically active alkaloids and their chemical analogues, peptides and inhibitors or cofactors of cellular enzymes; b) synthetic and natural compounds which are inhibitors or stimulators of cellular processes such as: cellular metabolism, DNA replication, RNA transcription, RNA translation, RNA elongation and RNA processing, protein synthesis, protein processing, cellular differentiation, cellular division, ion channel transmission, cellular protein and RNA's transportation, processes of cellular oxidation and the like. 8. Biologically active compounds "T" and "PR" in claims 1, 2, 3 and 4 include but are not limited to cytolitical toxins and toxins . 9. Biologically active compounds "PR" which are synthesized according to the processes presented in claims 2 and 4 are selected from biologically active proteins and RNAs. 10. The biologically active proteins and peptides described in claims 2,4 and 8 are synthesized from shorter biologically inactive peptides (PAs) consisting of from 2 to 100 aminoacids and their synthetic analogues L, D or DL configuration at the alpha carbon atom which are selected from valine, leucine, alanine, glycine, tyrosine, tryptophan, tryptophan isoleucine, proline, histidine, lysin, glutamic acid, methionine, serine, cysteine, glutamine phenylalanine, methionine sulf oxide, threonine, arginine, aspartic acid, asparagin, phenylglycine, norleucine, norvaline, alpha- aminobutyric acid, O-methylserine, O-ethylserine, S-methylcysteine , S-benzylcysteine , S- ethylcysteine, 5 , 5 , 5-trif luoroleucine and hexaf luoroleucine; other modifications of aminoacids are also possible, including but not limited to the addition of substituents at carbone atoms such as -OH, -SH, -SCH3, -OCH3 , -F,-Cl,-Br, -NH2 , -C(S)- or - C(O)-. 11. The biologically active proteins described in claims 8 and 9 include but are not limited to enzymes, DNA polymerases, RNA polymerases, esterases, lipases, proteases, kinases, transferases, transcription factors, transmembrane proteins, membrane proteins, cyclins, cytoplasmic proteins, nuclear proteins, toxins and like this. 12. The biologically active RNAs described in Formula 2 can be synthesized from biologically inactive oligoribonucleotides consisting of from 2 to 100 ribonucleotides , selected from uridin, guanidine, cytosin or adenine. 13. In claims 1 and 2, the cells where the biologically active substances can be synthesized have specific RNA, DΝA or dsDΝA molecules of determined sequence.

Inventors: SERGEEV PAVEL, SERGEEV, PAVEL
Applicants: SERGEEV PAVEL, SERGEEV, PAVEL
Application year: 1999
Main classification: C12P1
IPC codes: C12N15, C12P1, C12P17