A PROCESS OF DELIVERING A POLYNUCLEOTIDE TO A CELL VIA THE VASCULAR SYSTEM

Abstract: A process for delivering a polynucleotide into a parenchymal cell in a mammal, comprising, transporting the polynucleotide into a blood vessel communicating with the parenchymal cell in tissue or organ of the mammal such that the polynucleotide is transfected into the parenchymal cell. More specifically, the parenchymal cell consists of a parenchymal cell and the polynucleotide consists of a viral vector.

Description: A Process Of Delivering A Polynucleotide To A Cell Via The Vascular System Field of the Invention The invention generally relates to techniques for transferring genes into mammalian parenchymal cells in vivo More particularly, a method is provided for transfecnng parenchymal cells with polynucleotides delivered intravascularly Background of the Invention It was first observed that the in vivo injection of plasmid DNA into muscle enabled the expression of foreign genes m the muscle (Wolff, J A, Malone, R W, Williams, P, et al Direct gene transfer into mouse muscle in vivo Science 1990,247 1465-1468 ) Since that report, several other studies have reported the ability for foreign gene expression following the direct injection of DNA into the parenchyma of other tissues Naked DNA was expressed following its injection into cardiac muscle (Acsadi, G , Jiao, S , Jam, A , Duke, D , Williams, P , Chong, W , Wolff, J A Direct gene transfer and expression into rat heart m vivo The New Biologist 3(1), 71-81, 1991 ), pig epidermis (Hengge, U R , Chan, E F , Foster, R. A , Walker, P S , and Vogel, J C Nature Genetics 10 161-166 (1995)), rabbit thyroid (M Sikes, B O'Malley, M Fmegold, and F Ledley, Hum Gene Ther 5, 837 (1994), lung by lntratracheal injection (K B Meyer, M M Thompson, M Y Levy, L G Barron, F C Szoka, Gene Ther 2, 450 (1995)), into arteries using a hydrogel-coated angioplasty balloon (R Riessen et al, Human Gene Ther 4, 749 (1993)) (G Chapman et al Circ Res 71, 27 (1992)), melanoma tumors (R G Vile and I R Hart, Cancer Res 53, 962 (1993)) and rat liver [(Malone, R W et al JBC 269 29903-29907 (1994)) (Hickman, M A Human Gene Therapy 5 1477-1483 (1994))] Another important target tissue for gene therapy is the mammalian liver, given its central role in metabolism and the production of serum proteins A variety of techniques have been developed to transfer genes into the liver Cultured hepatocytes have been genetically modified by retroviral vectors [(Wolff, J A et al PNAS 84 3344-3348 (1987) (Ledley, F D , Darlington, G J , Hahn, T and Woo, S C L PNAS 84 5335-5339 (1987)] and re-implanted back into the livers in animals and in people [(J R Chowdhury et al Science 254, 1802 (1991) (M Grossman et al Nature Genetics 6, 335 (1994)] Retroviral vectors have also been delivered directly to livers in which hepatocyte division was induced by partial hepatectomy [(Kay, M A et al Hum Gene Ther 3 641-647 (1992) (Ferry, N , Duplessis, O , Houssin, D , Danos, O and Heard, J -M PNAS 88 8377-8381 (1991) (Kaleko, M , Garcia, J V and Miller, A D Hum Gene TΗer 2 27-32 (1991)] The injection of adenoviral vectors into the portal or systemic circulatory systems leads to high levels of foreign gene expression that is transient [(L D Stratford-Perπcaudet, M Levrero, J F Chasse, M Pemcaudet, P Bπand, Hum Gene Ther 1, 241 (1990) (H A Jaffe et al Nat Genet 1, 372 (1992) (Q Li, M A Kay, M Fmegold, L D Stratford-Pemcaudet, S L C Woo, Hum Gene Ther 4, 403 (1993)] Non-viral transfer methods have included polylysine complexes of asialoglycoprotems that are injected into the system circulation [Wu, G Y and Wu, C H J Biol Chem 263 14621-14624 (1988)] Foreign gene expression has also been achieved by repetitively injecting naked DNA in lsotomc solutions into the liver parenchyma of animals treated with dexamethasone [(Malone, R W et al JBC 269 29903-29907 (1994) (Hickman, M A Human Gene Therapy 5 1477- 1483 (1994)] Plasmid DNA expression in the liver has also been achieved via liposomes delivered by tail vein or intraportal routes [(Kaneda, Y , Kummitsu, I and Uchida, T J Biol Chem 264 12126-12129 (1989) (Soriano, P et al PNAS 80 7128-7131 (1983) Kaneda, Y , Iwai, K and Uchida, T Science 243 375-378 (1989)] Despite this progress, there is still a need for a gene transfer method that can efficiently and safely cause the expression of foreign genes in the liver m a and/or repetitive manner Summary of The Invention The present invention provides for the transfer of polynucleotides into parenchymal cells within tissues in situ and in vivo An mtravascular route of administration enables a prepared polynucleotide to be delivered to the parenchymal cells more evenly distributed and more efficiently expressed than direct parenchymal injections The efficiency of polynucleotide delivery and expression was increased substantially by increasing the permeability of the tissue's blood vessel This was done by increasing the lntravascular hydrostatic (physical) pressure and/or increasing the osmotic pressure Expression of a foreign DNA was obtained in mammalian liver by intraportally injecting plasmid DNA in a hypertonic solution and transiently clamping the hepatic vein/infenor vena cava Optimal expression was obtained by clamping the portal vein and injecting the hepatic vein/infenor vena cava A process is descnbed for dehvenng a polypeptide into a parenchymal cell in a mammal, compnsing, transporting the polynucleotide into a vessel communicating with the parenchymal cell of the mammal such that the polynucleotide is transfected into the parenchymal cell A process for dehvenng a coded polynucleotide into a parenchymal cell of a mammal for expression of a protein, comprising, transporting the polynucleotide to a vessel containing a fluid and having a permeable wall, and, increasing the permeability of the wall for a time sufficient to complete delivery of the polynucleotide Brief Description of the Drawings Fig. 1 Comparison of total luciferase expression in mice injected with pCILUC under vanous conditions The condition abbreviations signify the following PV-CL, portal vein injections without clamping, PV+CL, portal vein injections with clamping the hepatic vein, rVC-CL, JVC injections without clamping, TVC+CL, IVC injections with clamping the portal vein and hepatic artery, BD-CL, bile duct injections without clamping, BD+CL, bile duct injections with clamping the hepatic vem Numbers above the bars indicate the number of mice T bars indicate the standard error Fig. 2 Scatterplot of the log(e) of luciferase (ng protein) versus peak, lntrahepatic, parenchymal pressure (mm Hg) The solid line indicates the best fit Dotted lines indicate 95% prediction intervals Fig. 3 Histochemical analysis of β-galactosidase expression in livers after injection of pCLLacZ into a) mouse bile duct with the hepatic vein clamped, b) mouse IVC with the portal vein clamped and c) rat portal vein with the IVC clamped Mouse injections were done using 100 μg of pCILacZ in 1 ml of 15% mannitol and 2 5 units hepaπn/ml in normal saline solution while the rat injections were done using 750 μg of pCLLacZ m 15 ml of the same solution Panels A and B were magnified 160X and panel C was magnified 100X Fig.4 Human growth hormone levels (Hgh) following the repetitive administration of lOOμg of pCMVhGH into the bile duct of mice via a cannula Numbers identify individual mice Fig.5(a), (b), (c) Effects of injection solution volume (a), injection time (b) and premjection eschemia time (c) on the mean levels of luciferase expression in rat hind mb 3 muscles Two days after 475 ug of pCILux in 9 5 ml of NSS were injected unilaterally into the iliac artery of adult Sprague-Dawley rats, luciferase activities in 6 hindlimb muscle groups (anterior upper leg, medial upper leg, posterior upper leg, anterior lower leg, posteπor lower leg and foot) were measured as previously reported and presented as the means of the total luciferase levels of all 6 muscle groups The pre-injection ischemia time was 10 mm in "a" and "b" Numbers adjacent to data points indicate the number of animals (1 limb/animal) assayed for each condition Error bars indicate the standard error Fig. 6 Effects of vanous conditions on luciferase expression in rat hind limbs muscles followmg the injection of pCILux DNA into the iliac artery Condition 1, 475 ug DNA in 9 5 ml of NSS were mjected within 10 sec after a 10 mm occlusion of limb blood flow, Condition 2, 475 ug DNA m 9 5 ml of H2O were injected within 10 sec after a 10 min occlusion of limb blood flow, Condition 3, 475 ug DNA in 9 5 ml of NSS with 15% mannitol were withm 30 sec after a 10 mm occlusion of limb blood flow, Condition 4, 80 ug of collagenase m 1 ml of NSS were injection mto iliac artery immediately after blood flow occlusion After 10 mm, blood flow was unclosed for several sec, closed again, and 475 ug DNA in 9 5 ml of NSS were mjected withm 10 sec Bold numbers indicate the number of animals assayed at each experimental conditions Error bars indicate the standard error Detailed Description A Definitions The term, naked polynucleotides, indicates that the polynucleotides are not associated with a transfection reagent or other delivery vehicle that is required for the polynucleotide to be delivered to the parenchymal cell A transfection reagent is a compound or compounds used m the pnor art that bmd(s) to or complex(es) with polynucleotides and mediates their entry into cells The transfection reagent also mediates the binding and lnternalization of polynucleotides into cells Examples of transfection reagents include cationic liposomes and hpids, calcium phosphate precipitates, and polylysine complexes Typically, the transfection reagent has a net positive charge that binds to the polynucleotide's negative charge The transfection reagent mediates binding of polynucleotides to cell via its positive charge (that binds to the cell membrane's negative charge) or via ligands that bind to receptors m the cell For example, cationic liposomes or polylysine complexes have net positive charges that enable them to bind to DNA Other vehicles are also used, in the pnor art, to transfer genes into cells These include complexing the polynucleotides on particles that are then accelerated into the cell This is termed biohstic or gun techniques Other methods include eletroporation in which a device is used to give an electπc charge to cells The charge increases the permeability of the cell The term polynucleotide is a term of art that refers to a string of at least two base- sugar-phosphate combinations Nucleotides are the monomeπc units of nucleic acid polymers The term mcludes deoxyπbonucleic acid (DNA) and πbonucleic acid (RNA) m the form of an oligonucleotide messenger RNA, anti-sense, plasmid DNA, parts of a plasmid DNA or genetic mateπal deπved from a virus A polynucleotide is distinguished, here, from a oligonucleotide by containing more than 120 monomeπc units Anti-sense is a polynucleotide that interferes with the function of DNA and/or RNA A polynucleotide is considered m this specification to include a non-natural polynucleotide (not occumng in nature), for example a deπvative of natural nucleotides such as phosphothionates or peptide nucleic acids A polynucleotide can be delivered to a cell in order to produce a cellular change that is therapeutic The delivery of polynucleotides or other genetic mateπal for therapeutic purposes (the art of improvmg health m an ammal including treatment or prevention of disease) is gene therapy The polynucleotides are coded to express a whole or partial protein, or may be antisense, and can be delivered either directly to the organism m situ or indirectly by transfer to a cell that is then transplanted into the organism The protein can be missing or defective in an organism as a result of genetic, mheπted or acquired defect m its genome For example, a polynucleotide may be coded to express the protein dystrophin that is missing or defective in Duchenne muscular dystrophy The coded polynucleotide is delivered to a selected group or groups of cells and incorporated into those cell's genome or remain apart from the cell's genome Subsequently, dystrophm is produced by the formerly deficient cells Other examples of imperfect protein production that can be treated with gene therapy include the addition of the protein clotting factors that are missing in the hemophilias and en∑ymes that are defective m inborn eπors of metabolism such as phenylalanme hydroxylase A delivered polynucleotide can also be therapeutic in acquired disorders such as neurodegenerative disorders, cancer, heart disease, and infections The polynucleotide has its therapeutic effect by enteπng the cell Entry into the cell is required for the polynucleotide to produce the therapeutic protein, to block the production of a protein, or to decrease the amount of a RNA Additionally, a polynucleotide can be delivered to block gene expression Such polynucleotides can be anti-sense by preventing translation of a messenger RNA or could block gene expression by preventing transcπption of the gene Preventing both RNA translation as well as DNA transcπption is considered preventing expression Transcπption can be blocked by the polynucleotide binding to the gene as a duplex or tπplex It could also block expression by binding to proteins that are involved in a particular cellular biochemical process Polynucleotides may be delivered that recombine with genes The polynucleotides may be DNA, RNA, hybπds and deπvatives of natural nucleotides Recombine is the mixing of the sequence of a delivered polynucleotide and the genetic code of a gene Recombine includes changing the sequence of a gene Delivery of a polynucleotide means to transfer a polynucleotide from a container outside a mammal to withm the outer cell membrane of a cell m the mammal The term transfection is used herein, in general, as a substitute for the term delivery, or, more specifically, the transfer of a polynucleotide from directly outside a cell membrane to within the cell membrane If the polynucleotide is a pπmary RNA transcπpt that is processed into messenger RNA, a πbosome translates the messenger RNA to produce a protein withm the cytoplasm If the polynucleotide is a DNA, it enters the nucleus where it is transcπbed into a messenger RNA that is transported mto the cytoplasm where it is translated into a protein The polynucleotide contains sequences that are required for its transcπption and translation These include promoter and enhancer sequences that are required for initiation DNA and thus the corresponding messenger RNA (transcπbed from the DNA) contains introns that must be spliced, poly A addition sequences, and sequences required for the initiation and termination of its translation mto protein Therefore if a polynucleotide expresses its cognate protein, then it must have entered a cell A therapeutic effect of the protein in attenuating or preventing the disease state can be accomplished by the protein either staying within the cell, remaining attached to the cell in the membrane or bemg secreted and dissociating from the cell where it can enter the general circulation and blood Secreted proteins that can be therapeutic include hormones, cytokmes, growth factors, clotting factors, anti-protease proteins (e g alpha-antitrypsin) and other proteins that are present in the blood Proteins on the membrane can have a therapeutic effect by providing a receptor for the cell to take up a protein or lipoprotem For example, the low density lipoprotem (LDL) receptor could be expressed in hepatocytes and lower blood cholesterol levels and thereby prevent atherosclerotic lesions that can cause strokes or myocardial infarction Therapeutic proteins that stay within the cell can be enzymes that clear a circulating toxic metabolite as in phenylketonuπa They can also cause a cancer cell to be less proliferative or cancerous (e g less metastatic) A protein within a cell could also interfere with the replication of a virus The delivered polynucleotide can stay withm the cytoplasm or nucleus apart from the endogenous genetic material Alternatively, the polynucleotide could recombine (become a part of) the endogenous genetic material For example, DNA can insert into chromosomal DNA by either homologous or non-homologous recombination Parenchymal cells are the distinguishing cells of a gland or organ contained in and supported by the connective tissue framework The parenchymal cells typically perform a function that is unique to the particular organ The term "parenchymal" often excludes cells that are common to many organs and tissues such as fibroblasts and endothelial cells withm the blood vessels In a liver organ, the parenchymal cells include hepatocytes, Kupffer cells and the epithelial cells that line the biliary tract and bile ductules The major constituent of the liver parenchyma are polyhedral hepatocytes (also known as hepatic cells) that presents at least one side to an hepatic sinusoid and apposed sides to a bile canahculus Liver cells that are not parenchymal cells include cells within the blood vessels such as the endothelial cells or fibroblast cells In stπated muscle, the parenchymal cells include myoblasts, satellite cells, myotubules, and myofϊbers In cardiac muscle, the parenchymal cells include the myocardium also known as cardiac muscle fibers or cardiac muscle cells and the cells of the impulse connecting system such as those that constitute the sinoatπal node atπoventπcular node, and atπoventπcular bundle 7 In a pancreas, the parenchymal cells mclude cells withm the acini such as zymogemc cells, centroacinar cells, and basal or basket cells and cells within the islets of Langerhans such as alpha and beta cells In spleen, thymus, lymph nodes and bone marrow, the parenchymal cells mclude reticular cells and blood cells (or precursors to blood cells) such as lymphocytes, monocytes, plasma cells and macrophages In the nervous system which includes the central nervous system (the brain and spinal cord) peπpheral nerves, and ganglia, the parenchymal cells include neurons, glial cells, microglial cells, oligodendrocytes, Schwann cells, and epithelial cells of the choroid plexus In the kidney, parenchymal cells include cells of collecting tubules and the proximal and distal tubular cells In the prostate, the parenchyma includes epithelial cells In glandular tissues and organs, the parenchymal cells include cells that produce hormones In the parathyroid glands, the parenchymal cells include the pπncipal cells (chief cells) and oxyphilic cells In the thyroid gland, the parenchymal cells include follicular epithelial cells and parafollicular cells In the adrenal glands, the parenchymal cells mclude the epithelial cells within the adrenal cortex and the polyhedral cells withm the adrenal medulla In the parenchyma of the gastrointestinal tract such as the esophagus, stomach, and intestines, the parenchymal cells mclude epithelial cells, glandular cells, basal, and goblet cells In the parenchyma of lung, the parenchymal cells mclude the epithelial cells, mucus cells, goblet cells, and alveolar cells In fat tissue, the parenchymal cells include adipose cells or adipocytes In the skm, the parenchymal cells mclude the epithelial cells of the epidermis, melanocytes, cells of the sweat glands, and cells of the hair root In cartilage, the parenchyma mcludes chondrocytes In bone, the parenchyma includes osteoblasts, osteocytes, and osteoclasts An mtravascular route of administration enables a polynucleotide to be delivered to parenchymal cells more evenly distributed and more efficiently expressed than direct parenchymal injections Intravascular herem means within a hollow tubular structure called a vessel that is connected to a tissue or organ withm the body Within the cavity of the tubular structure, a bodily fluid flows to or from the body part Examples of bodily fluid include blood, lymphatic fluid, or bile Examples of vessels mclude arteπes, artenoles, capillaπes, venules, sinusoids, veins, lymphatics, and bile ducts The lntravascular route includes delivery through the blood vessels such as an artery or a vein Polypeptide refers to a linear seπes of amino acid residues connected to one another by peptide bonds between the alpha-ammo group and carboxy group of contiguous ammo acid residues Protein refers to a linear series of greater than 50 amino acid residues connected one to another as m a polypeptide Vectors are polynucleic molecules originating from a virus, a plasmid, or the cell of a higher organism into which another nucleic fragment of appropnate size can be integrated without loss of the vectors capacity for self- replication, vectors introduce foreign DNA into host cells, where it can be reproduced Examples are plasmids cosmids, and yeast artificial chromosomes, vectors are often recombinant molecules containing DNA sequences from several sources A vector includes a viral vector for example, adenovirus (icosahedral (20-sided) virus that contains, there are over 40 different adenovirus vaneties, some of which cause the common cold) DNA, adenoassociated viral vectors (AAV)whιch are deπved from adenoassociated viruses and are smaller than adenoviruses, and retrovirus (any virus m the family Retroviπdae that has RNA as its nucleic acid and uses the enzyme reverse transcπptase to copy its genome into the DNA of the host cell's chromosome, examples include VSV G and retroviruses that contain components of lentivirus including HTV type viruses) Afferent blood vessels of organs are defined as vessels which are directed towards the organ or tissue and in which blood flows towards the organ or tissue under normal physiologic conditions Conversely, the efferent blood vessels of organs are defined as vessels which are directed away from the organ or tissue and in which blood flows away from the organ or tissue under normal physiologic conditions In the liver, the hepatic vein is an efferent blood vessel since it normally cames blood away from the liver into the infeπor vena cava Also in the liver, the portal vein and hepatic arteπes are afferent blood vessels in relation to the liver since they normally carry blood towards the liver B Delivery of Polynucleotides In a prefeπed embodiment of the present invention, a naked polynucleotide is delivered mto a liver blood vessel at distal or proximal points A liver blood vessel includes the portal venous system which transports blood from the gastrointestinal tract and other internal organs (e g spleen, pancreas and gall bladder) to the liver Another liver blood vessel is the hepatic vein The hepatic vein may also be reached via the infeπor vena cava or another blood vessel that ultimately connects to the liver A needle or catheter is used to mject the polynucleotide into the vascular system The injection can be performed under direct observation following an incision and visualization of the tissues blood vessels Alternatively, a catheter can be inserted at a distant site and threaded so that it resides in the vascular system that connects with the target tissue In another embodiment, the injection could be performed by usmg a needle that traverses the intact skm and enters a vessel that supplies or drains from the target tissue In a preferred embodiment, the liver and portal vein of mice (25 g, 6-week old ICR mice) are visualized through a ventral midline incision Anesthesia was obtained from intramuscular injections of 1000 μg of ketamine-HCl (Parke-Davis, Moms Plains, NJ) in 1 ml of normal saline and methoxyflurane (Pitman-Moore, Mudelein, LL USA) winch was administered by inhalation as needed Plasmid DNA in 1 ml of vaπous solutions containing hepaπn to prevent clotting was injected into the portal vein using a needle over approximately 30 sec At vaπous times after the injection, the animals were sacπficed by cervical dislocation and the livers (average weight of 1 5 g) were divided into six sections composed of two pieces of median lobe, two pieces of left lateral lobe the right lateral lobe, and the caudal lobe plus a small piece of πght lateral lobe Each of the six sections were placed separately into an homogenizing buffer The homogenates were centrifuged and the supernatant analyzed for the foreign gene product If the gene product is secreted then blood is obtained from the retro- orbital venous sinus and the level of the secreted protein is assayed in the blood For example, the expression of the human growth hormone gene can be detected by measuring the amount of human growth hormone in the mouse serum using a radioimmune assay (RIA) (HGH- TGES 100T kit from Nichols Institute, San Juan Capistrano, CA, USA) Alternatively, the foreign gene could produce an enzyme that corrects an abnormality the disease state For example, the phenylalamne hydroxylase gene could be used to normalize the elevated phenylalanine blood levels in a genetic mouse model of phenylketonuπa 10 In the liver, the hepatic vein is an efferent blood vessel smce it normally cames blood away from the liver mto the infeπor vena cava Also in the liver, the portal vein and hepatic arteπes are afferent blood vessels in relation to the liver since they normally carry blood towards the liver In a prefeπed embodiment, plasmid DNA may be efficiently expressed if delivered by a retrograde route mto the efferent vessel of the liver (1 e the hepatic vein) As demonstrated in the examples that follow, injections were directed mto the mfeπor cava winch was clamped in two locations, proximal and distal to the entry of the hepatic vein into the infeπor vena cava Specifically, the downstream inferior vena cava clamp was placed between the diaphragm and the entry point of the hepatic vein The upstream infenor vena cava clamp was placed j ust upstream of the entry point of the renal veins Smce the veins of other organs such as the renal vems enter the mfeπor vena cava at this location, not all of the injection fluid went into the liver In some of the animals that received retrograde injections in the infeπor vena cava, the hepatic artery, mesenteπc artery and portal vein were clamped (occluded) C Permeability The efficiency of the polynucleotide delivery and expression was increased substantially by mcreasing the permeability of a blood vessel within the target tissue Permeability is defined here as the propensity for macromolecules such as polynucleotides to move through vessel walls and enter the extravascular space One measure of permeability is the rate at which macromolecules move through the vessel wall and out of the vessel Another measure of permeability is the lack of force that I esists the movement through the vessel wall and out of the vessel Vessels contain elements that prevent macromolecules from leaving the lntravascular space (internal cavity of the vessel) These elements include endothelial cells and connective mateπal (e g collagen) High permeability indicates that there are fewer of these elements that can block the egress of macromolecules and that the spaces between these elements are larger and more numerous In this context, high permeability enables a high percentage of polynucleotides being delivered to leave the lntravascular space, while low permeability indicates that a low percentage of the polynucleotides will leave the lntravascular space The permeability of a blood vessel can be increased by increasing the lntravascular hydrostatic pressure In a prefeπed embodiment the lntravascular hydrostatic pressure is mcreased by rapidly (from 10 seconds to 30 minutes) injecting a polynucleotide in solution into the blood vessel which increases the hydrostatic pressure In another preferred 1 1 embodiment, hydrostatic pressure is mcreased by obstructing the outflow of the injection solution from the tissue for a peπod of time sufficient to allow delivery of a polynucleotide Obstructing means to block or impede the outflow of injection fluid, thereby transiently (reversibly) blocking the outflow of the blood Furthermore, rapid injection may be combined with obstructing the outflow in yet another preferred embodiment For example, an afferent vessel supplying an organ is rapidly injected and the efferent vessel draining the tissue is ligated transiently The efferent vessel (also called the venous outflow or tract) draining outflow from the tissue is also partially or totalK clamped for a peπod of time sufficient to allow delivery of a polynucleotide In the reverse, an efferent is injected and an afferent vessel is occluded In another preferred embodiment, the lntravascular pressure of a blood vessel is increased by increasing the osmotic pressuie within the blood vessel Typically, hypertonic solutions containing salts such as NaCl sugars or polyols such as mannitol are used Hypertonic means that the osmolahty of the injection solution is greater than physiologic osmolahty Isotomc means that the osmolahtv of the injection solution is the same as the physiological osmolahty (the tonicity or osmotic pressure of the solution is similar to that of blood) Hypertonic solutions have increased tonicity and osmotic pressure similar to the osmotic pressure of blood and cause cells to shnnk The permeability of the blood vessel can also be mcreased by a biologically-active molecule in another prefeπed embodiment A. biologically-active molecule is a protein or a simple chemical such as histamine that increases the permeability of the vessel by causing a change in function, activity, or shape of cells within the vessel wall such as the endothelial or smooth muscle cells Typically, biologicallv-active molecules interact with a specific receptor or enzyme or protein within the vascular cell to change the vessel's permeability Biologically- active molecules include vascular permeability factor (VPF) which is also known as vascular endothelial growth factor (VEGF) Another type of biologically-active molecule can also increase permeability by changing the extracellular connective mateπal For example, an enzyme could digest the extracellular mateπal and increase the number and size of the holes of the connective mateπal 12 EXAMPLES The following examples are intended to illustrate, but not limit, the present invention. Example 1 Intraportal injections of plasmid DNA: Methods: After the livers of 25 g, 6-week old mice were exposed through a ventral midline incision, solutions containing pBS CMVLux plasmid DNA (described below) were manually injected over approximately 30 sec into the portal vein using a 30-gauge, 1/2 -inch needle and 1-ml syπnge. In some animals, a 5 X 1 mm, Kleinert-Kutz microvessel clip (Edward Week, Inc., Research Triangle Park, NC) was applied during the injection at the junction of the hepatic vein and caudal vena cava Anesthesia was obtained from intramuscular injections of 1000 μg of ketamine-HCl (Parke-Davis, Moπis Plains, NJ) in 1 ml of normal saline and methoxyflurane (Pitman-Moore, Mudelein, LL USA) which was administered by inhalation as needed, was purchased from Sigma. Hepaπn was purchased from LyphoMed (Chicago, H). Reporter Genes and Assays The pBS.CMVLux, plasmid DNA was used to express luciferase from the human immediate early cytomegalovirus (CMV) promoter (I. Danko, et al.,Gene Therapy 1, 114 (1994) incorporated herein by reference) At two days after injection, the livers were assayed for luciferase expression as previously reported (J A. Wolff, et al., Science 247, 1465 (1990)) except modified as below. The animals were sacrificed by cervical dislocation and the livers (average weight of 1.5 g) were divided into six sections composed of two pieces of median lobe, two pieces of left lateral lobe, the right lateral lobe, and the caudal lobe plus a small piece of right lateral lobe. Each of the six sections were placed separately mto 200 μl of lysis buffer (0.1% Triton X-100, 0. IM K-phosphate. 1 mM DTT pH 7.8) that was then homogenized using a homogenizer PRO 200 (PRO Scientific Inc., Monroe CT). The homogenates were centrifuged at 4,000 rpm for 10 min. at 4 ° C and 20 μl of the supernatant were analyzed for luciferase activity Relative light units (RLU) weie converted to pg of luciferase usmg standards from Analytic Luminescence Laboratories (ALL, San Diego, CA) Luciferase protein (pg) = 5 1 X 10 "5 X RLU + 3 683 (r 2 = 0 992) Total luciferase/liver was calculated by adding all the sections of each liver and multiplying by 23 to account for dilution effects For each condition, the mean total luciferase/liver and the associated standard deviation are shown Results: After the livers of 25 g, 6-week old mice were exposed through a ventral midline incision, 100 μg of pBS CMVLux, plasmid DNA in 1 ml of solutions was injected into the portal vein via a 30-gauge, 1/2 -inch needle over approximately 30 sec Two days after injection, a mean of only 0 4 ng of total luciferase/liver was produced when the DNA was delivered lntraportally m an lsotonic solution without ligation of the hepatic vein (Table 1) Inclusion of 20% manmtol in the injection solution increased the mean total luciferase/liver over ten-fold to 4 8 ng (Table 1) In order to prevent the DNA's rapid transit and to increase the intraportal hydrostatic pressure, the hepatic vein was clamped for two min after injection Luciferase production increased another three-fold to 14 7 ng (Table 1 ) When the DNA was injected in a hypertonic solution containing 0 9% saline, 15% manmtol and 2 5 units/ml of hepaπn to prevent microvascular thrombosis and with the hepatic vein clamped, luciferase expression increased eight-fold to 120 3 ng/liver (Table 1) These results are also shown in Table 7 (no dexamethasone condition) in Example 3 below for each individual animal If the manmtol was omitted under these conditions, luciferase expression was ten-fold less (Table 1) These results indicate that hypertoniciu hepaπn and hepatic vein closure are required to achieve very high levels of luciferase expression Table 1 Mean total luciferase in the liver following the intraportal injection (over 30 seconds) of 100 μg pBS CMVLux in 1 ml of different solutions with no clamp or with the hepatic vein and infeπor vena cava clamped for two minutes 14 Condition Mean Standard Error Number of Luciferase Livers (total ng/liver) no clamp, normal saline solution 0 4 0 7 n=6 (NSS) no clamp, 20% manmtol 4 8 8 1 n=3 clamp, 20% manmtol 14 6 26 3 n=9 clamp, 2 5 units hepann/ml m 11 8 12 5 n=4 NSS clamp, 15%) manmtol and 2 5 120 3 101 5 n=12 units hepann/ml m NSS Luciferase activities in each liver were evenly distπbuted m six divided sections assayed (Table 2) All six parts of each liver from all three ammals had substantial amounts of luciferase This is in marked contrast to the direct interstitial, mtralobar injection of DNA in which the expression is restncted to the site of injection (R W Malone et al , J Biol Chem 269, 29903 (1994), M A Hickman, et al , Hum Gene Ther 5, 1477 (1994) incorporated herein by reference) Table 2 The distπbution of luciferase expression over the six liver sections in ammals injected lntraportally (over 30 seconds) with 100 μg of pBS CMVLux in 1 ml of normal saline solution plus 15% manmtol and 2 5 umts hepann/ml and with the hepatic vein clamped for 2 minutes Liver Section Total luciferase/Liver (ng/Liver/mouse) Mouse #1 Mouse #2 Mouse #3 1/2 of median lobe 496 5 66 9 304 5 other 1/2 of median 177 0 126 1 241 4 lobe 1/2 of left lateral 763 8 208 7 325 2 lobe other 1/2 of left 409.4 160.4 218.9 lateral lobe right lateral lobe 527.8 129.7 216.2 caudal lobe + small 374.1 149.7 240.8 piece of right lateral lobe Total 2,748.6 841.5 1,547.0 Mean 458.1 140.3 257.8 Range 177-763 67-209 216-325 Standard Deviation 194.0 46.6 45.9 Conclusions: 1. High levels of luciferase expression were obtained from injecting 100 μg of pBS. CMVLux intraportally. 2. The highest levels of luciferase expression were obtained when the animals were injected intraportally over 30 seconds with 100 μg of pBS. CMVLux in 1 ml of normal saline solution plus 15%> mannitol and 2.5 umts hepann/ml and with the hepatic vein clamped for 2 minutes. 3. These high levels of expression were consistently obtained in dozens of mice. The luciferase expression was evenh distπbuted throughout the liver. Example 2 The effects of other factors on expression were explored using the same methods for the intraportal injection of pBS. CMVLux 16 Methods Unless otherwise specified, the intraportal injections and luciferase assays were done as in Example 1 Results Compared to the results with 100 μg of pBS CMVLUX, luciferase expression was not greater with 500 μg of plasmid DNA (Table 3) Luciferase expression was approximately 7- fold less if 20 μg of pBS CMVLux DNA was inj ected instead of 100 μg Table 3 Total luciferase expression in each liver of each animal injected intraportally (over 30 sec) with 20 μg , 100 μg , or 500 μg of pBS CMVLux in 1 ml of normal salme solution plus 15%) manmtol and 2 5 umts hepann/ml and with the hepatic vein occluded for 2 mm Total luciferase/Liver (ng/Liver/mouse) Mouse Number 100 μg pBS CMVLux 500 μg pBS CMVLux 1 1,023 15 2 178 82 3 108 23 4 140 340 Mean 362 115 Standard 441 153 Deviation The times for which the hepatic vein was occluded were vaπed from 2 mm to 4 mm and to 6 mm In Table 4, one can see that the time of occlusion did not have a large effect on expression Table 4 Effect of time of hepatic vein occlusion on luciferase expression in ammals injected intraportally with 100 μg of pBS CMVLux in 1 ml of normal saline solution plus 15% manmtol and 2 5 umts hepann/ml Mouse Number Total luciferase/Liver (ng/Liver/mouse) 2 mm 4 mm 6 mm 1 4 6 1 9 32 7 2 44 9 11 5 6 4 The times over which the injections were done were vaπed from 30 seconds to 1 mmute and 2 minutes In Table 5, one can see that injecting the 1 ml of the DNA solution (100 μg pBS CMVLux) over 30 seconds enabled the highest levels of luciferase expression Longer times of injection led to lower levels Table 5 Effect of length of mj ection (time it took to inject all of the 1 ml) on luciferase expression m animals mjected intraportally with 100 μg of pBS CMVLux m 1 ml of normal sahne solution plus 15% manmtol and 2 5 units hepann/ml and with the hepatic vem occluded for 2 mm Mouse Number Total luciferase Liver (ng/Liver/mouse) 30 sec 1 mm 2 mm 1 2,697 188 21 6 2 790 13 4 19 9 3 1,496 141 1 11 8 j Mean 1,662 114 18 Standard Deviation 964 91 5 If the total volume of the injection fluid was 0 5 ml instead of 1 0 ml, luciferase expression decreased 70-fold (Table 6) suggesting that 0 5 ml was not sufficient to fill the lntravascular space and distπbute the DNA throughout the parenchyma Table 6 Total luciferase expression in each liver of each ammal injected intraportally (over 30 sec) with 100 μg of pBS CMVLux in either 0 5 or 1 ml of normal salme solution plus 15% manmtol and 2 5 umts hepann/ml and with the hepatic vein occluded for 2 mm Total luciferase/Liver (ng/Liver/mouse) Mouse Number 0 5 ml 1 ml 1 1 6 51 9 2 4 7 124 8 3 0 4 266 9 Mean 2 3 147 9 Standard 2 3 109 4 Deviation Conclusions 1 The optimal conditions are in fact the conditions first descπbed in example 1 the ammals were injected intraportally over 30 seconds with 100 μg of pBS CMVLux in 1 ml of normal salme solution plus 15% manmtol and 2 5 umts hepann ml and with the hepatic vein clamped for 2 minutes 2 Use of 500 μg of pBS CMVLux did not enable greater levels of expression but expression was approximately 7-fold less if 20 μg of DNA was used 3 Occluding the hepatic vem for longer than 2 minutes did not increase expression 4 Injecting the pBS CMVLux over 30 seconds gave the highest luciferase levels as compared to injection times longer than 30 seconds 5 Injecting the pBS CMVLux in 1 ml gave higher luciferase levels than injecting the pBS CMVLux in 0 5 ml 19 Example 3 Methods The intraportal injections and luciferase assays were performed as in Example 1 except that some ammals received daily subcutaneous injections of 1 mg/kg of dexamethasone (Elkins-Smn, Cherry Hill, NJ) starting one day prior to surgery The conditions for the injections were intraportal injections over 30 seconds with 100 μg of pBS CMVLux in 1 ml of normal salme solution plus 15% manmtol and 2 5 umts hepann/ml and with the hepatic vem clamped for 2 minutes Results Under the conditions descπbed above (1 e , hypertonic solution containing hepaπn and hepatic vem closure) mto ammals that had been injected with daily mjections of dexamethasone starting the day pnor to plasmid injection, luciferase expression was three-fold greater than the expression without dexamethasone (Table 7) Table 7 The effect of dexamethasone mjections on luciferase expression after the intraportal injection of pBS CMVLux Total luciferase/Liver (ng/Liver/mouse) Mouse Number NO Dexamethasone WITH Dexamethasone 1 51 9 1,181 1 2 124 8 364 7 3 266 9 82 8 4 73 7 120 5 5 52 6 1,022 9 6 7 3 178 1 7 146 1 107 6 8 231 4 140 2 9 271 2 10 8 7 11 8 3 12 201 1 20 Mean 120 3 399 8 Standard 101 4 444 1 Deviation Dexamethasone could have increased the production of luciferase and the expression of other genes by several mechanisms They include increasing the amount of plasmid DNA that enters the liver cells by modifying the state of the liver cells It could also help the liver cells withstand the high pressure However, the most likely mechanism is that dexamethasone directly stimulates the CMV promoter and thereby directly increases expression of luciferase by stimulating transcπption of the luciferase messenger RNA The use of dexamethasone demonstrates that using a readily available pharmaceutical, the levels of expression can be substantially increased and regulated Conclusion 1 Dexamethasone administration increased luciferase expression from lntraportally- injected pBS CMVLux plasmid DNA three-fold 2 This demonstrates that the expression from the liver can be regulated using a commonly-used pharmaceutical Example 4 Methods The intraportal mjections were performed using the previously stated technique of mjections over 30 seconds with 100 μg of plasmid DNA in 1 ml of normal saline solution plus 15% manmtol and 2 5 umts hepann/ml and with the hepatic vem clamped for 2 minutes The mice also received daily subcutaneous injections of 1 mg/kg of dexamethasone (Elkins-Sinn, Cherry Hill, NJ) starting one day pnor to surgery The plasmids pBS CMVLacZ and pBS CMVnLacZ were used to express a cytoplasmic and nuclear β -galactosidase protein, respectively, from the CMV promoter 21 (Picard, D & Yamamoto, K EMBO J 6 3333-3340, 1987, incoφorated herein by reference) They were constructed by placing either a 3 5-kg-HιndIII/XbaI B-galactosidase sequence from pSDKLacZpa (Danko, I et al Gene Therapy 1 114-121, 1994, incorporated herein by reference) or a sequence encoding a nuclear-localizing -galactosidase (Picard, D & Yamamoto, K EMBO J 6 3333-3340, 1987, incoφorated herein by reference) into pBlueCMV (Danko, I et al Gene Therapy 1 114-121, 1994, incoφorated herein by reference) Two days after intraportal injection, the livers were perfused with 1% paraformaldehyde and 1 25% glutaraldehyde in phosphate buffered salme (PBS) and then kept in this solution for one day After the livers were stored in 30% sucrose, they were cryosectioned The sections were mounted on slides and stained for 1 hour to one day with a PBS solution (pH 7 5) containing 400 μg/ml X-gal (5-bromo-4-chloro-3-ιndolyl-β-D- galactoside) (Sigma), 5 mM potassium fern cyanide, 5 mM feπocyarude, and lmM MgCl2 After washing, the sections were then counter-stained with hematoxylm and eosin In the livers injected with the nuclear-localizing β-galactosidase vector, the washing step after hematoxylm incubation was omitted to decrease its nuclear staining Results Having defined the optimal conditions, the types and percentages of transfected cells were determined After injections of a 100 μg of the cytoplasmic (pBS CMVLacZ) or the nuclear (pBS CMVnLacZ) β-galactosidase expression vectors into dexamethasone-treated ammals, liver cryosections 10- to 30-μm thick were stained for β-galactosidase using X-gal at pH 7 5 to prevent background staimng Intense blue staming was observed in approximately 1%) of the liver cells and was evenly distributed throughout the liver X-gal incubations for only 1 hour resulted in intensely blue cells, suggesting that the transfected cells expressed relatively large amounts of the foreign genes Control livers mjected with 100 μg of pBS CMVLux did not contain any positively-stained cells Necrosis was observed in approximately 10% of the sections However, some livers with high β-galactosidase expression did not contain any sections with necrosis 22 The hepatocytes were identified by their characteπstic moφhology For example, many of the cells in the livers injected with the nuclear β-galactosidase vector, pBS CMVnLacZ, had blue staimng in two nuclei, which is a trait only of hepatocytes Although the majoπty of the positively-stained cells were hepatocytes a few small, non-hepatocyte cells contained blue staimng Conclusion 1 Approximately 1 % of the liver cells were transfected with the B-galactosidase gene throughout the entire liver 2 Almost all of the transfected liver cells were hepatocytes Example 5 Methods Luciferase expression in the liver was compared to that in cultured HepG2 hepatocytes in 35-mm plates Transfections were done using 3 μg of pBS CMVLux/plate and either 3 μg of Lipofectin (Life Technologies. Bethesda, MD) or 6 μg of LipofectAMLNE (Life Technologies, Bethesda, MD) per manufacturer's instructions Two days after transfection, 200 ul of lysis buffer was added to the cultures and 20 ul of the supernatant were analyzed for luciferase activity as m Example 1 Results The efficiency of luciferase expression using this technique was compared to other methods of gene transfer both in vitro and in vivo Transfections performed under optimal conditions with pBS CMVLUX and Lipofectin or LipofectAMLNE (Life Technologies Ine ) in HepG2 hepatocytes in culture (n=8) yielded a mean total of 3 7±4 5 ng lucιferase/35-mm plate 23 and 2 8±2 0 ng lucιferase/35-mm plate Thus the efficiency of transfection (without dexamethasone) in terms of ng of luciferase/ μg of pBS CMVLUX DNA was approximately 1 ng/μg both in vitro and in vivo The published procedure of repetitively and directly injecting naked plasmid DNA mto a rat liver lobe was reduced proportionately for mouse liver (R W Malone et al , J Biol Chem 269, 29903 (1994), M A Hickman, et al , Hum Gene Ther 5, 1477 (1994), incoφorated herein by reference) A total of 100 μg of pBS CMVLUX in a total volume of 200 ul of normal saline was injected within five different sites (40 ul/site) mto the left lateral lobe of 30 g mice treated with dexamethasone A mean total of only 0 1 ng/hver (4 livers, 0 001 ng luciferase/μg DNA) was obtained and the luciferase expression was only present in the injected lobe Approximately 30-fold more luciferase expression was obtained if the direct mtralobar injections were done usmg 1 ml of injection fluid and clamping the hepatic vein In the previous studies involving the multiple injections of a total of 500 μg of pCMVL mto a liver lobe of dexamethasone-treated rats, a mean of 9 87 ng of luciferase/liver (0 02 ng/μg DNA) was expressed (R W Malone et al , J Biol Chem 269, 29903 (1994), M A Hickman, et al , Hum Gene Ther 5, 1477 (1994)) With regard to muscle, we typically inject 10 ug of pBS CMVLUX or pBS RSVLUX ((Danko, I et al Gene Therapy 1 114-121 1994)) in normal sahne into 6-8 mouse quadπceps muscle per experiment In dozens of expenments, mean total luciferase per muscle was 04-1 ng (±0 5-1 2) and the efficiency was 0 04-0 1 ng luciferase/μg DNA Table 8 Compaπson of efficiency of gene transfer in terms of luciferase expressed per μg of pBS CMVLux plasmid DNA used for the method (ng luciferase/μg DNA) Method of Gene Transfer Mean Total Yield Of Amount of Efficiency (ng Luciferase (ng) pBS CMVLux Luciferase/μg Used (μg ) DNA) Intraportal Mouse Liver 120 3±101 5 100 1 2 (above optimal conditions- n=12 Table 1) hepatic vein clamped 24 HepG2 In Vitro with 3 7±4 5 3 1 2 Lipofectin (n=8) HepG2 In Vitro with 2 8±2 0 3 0 9 LipofectAMLNE (n=8) Intraiobar Mouse Liver (20 O l±O l 100 0 001 ul/site X 5 sites) n=4 hepatic vem not clamped Intraiobar Mouse Liver 2 8±5 6 100 0 028 (1 ml/1 site) n=4 hepatic vein clamped Intraiobar Rat Liver 9 87 500 0 02 (5 sites) from published data (Journal of Biologic Chemistry 269 29903, 1994, Human Gene Therapy 5 1477, 1994) Intramuscular 0 4-1 10 0 04-0 1 ±0 5-1 2 n>50 Conclusions 1 The intraportal delivery of naked DNA was more than an order of magnitude more efficient than mterstitial delivery into either liver or muscle and more evenly distributed Example 6 Methods The intraportal injections were done using the above optimal injections which are intraportal injections over 30 seconds with 100 μg of pCMVGH m 1 ml of normal salme solution plus 15% manmtol and 2 5 units heparm/ml and with the hepatic vein clamped for 2 25 minutes Some ammals received daily intramuscular mjections of 100 mg/kg of cyclospoπne (Sandimmune, Sandoz) or daily subcutaneous injections of 1 mg/kg of dexamethasone (Elkms- Sinn, Cherry Hill, NJ), or both starting one day prior to surgery The previously descπbed pCMVGH plasmid DNA was used to express human growth hormone (hGH) (C Andree, et al , Proc Natl Acad Sci U S A 91, 12188 (1994), incoφorated herem by reference) Blood obtained from the retro-orbit sinus was analyzed for serum concentration of hGH using the radioimmune assay (RIA), HGH-TGES 100T kit from Nichols Institute (San Juan Capistrano, CA) Results Human growth hormone (hGH) was used as a marker gene to assess the ability of this gene transfer technique to produce a therapeutic serum protein (Table 9) Two days after the intraportal injection of 100 μg of pCMVGH under the above optimal conditions, the mean hGH serum concentration was 57±22 ng/ml (n=12) with a range of 21-95 ng/ml Neither dexamethasone nor cyclospoπne pre-treatment sigmficantiy affected these imtial hGH levels In two ammals injected with pBS CMVLUX, background hGH levels were 03 ± 0 1 ng/ml for 4 weeks afterwards In humans, normal pulsatile levels of GH peak at approximately 20 ng/ml above baseline values of approximately 1 ng/ml and can attain concentrations of 10-180 ng/ml after growth hormone releasing hormone (GHRH) stimulation (A Favia, J D Veldhuis, M O Thorner, M L Vance, J Chn Endocπnol Metab 68, 535 (1989), R W Holl, M L Hartman, J D Veldhuis, W M Taylor, M O Thomer, J Chn Endocπnol Metab 72, 854 (1991), W S Evans et al , Am J Physiol 252, E549 (1987), F P Alford, H W G Baker, H. G Burger, J Chn Endocnnol Metab 37 515(1973), incoφorated herein by reference) The half-life of hGH is approximately 20 mm in humans and 4 5 mm m mice, hence these serum levels could translate into much higher levels for more stable protems (S Peeters and H G Fnesen, Endocnnol 101, 1164 (1977), A Favia, J D Veldhuis, M O Thorner, M L Vance, J Chn Endocπnol Metab 68, 535 (1989), incoφorated herein by reference) For example, if a protein such as alpha-antitrypsin has a half-life that is ten times longer than human GH, then the circulating blood levels should be at more than ten times higher given the same efficiency of protein production Another example is that for hemophilia which requires levels of factor VJJJ or IX in the range of approximately 1 μg of the clotting factor/ml of 26 blood Given the increased stability of these clotting factors, then the 0 1 μg /ml of hGH that we can achieve after intraportal injection of the respective gene means that we would be able to obtain therapeutic levels of clotting factors to prevent bleeding in patients with hemophilia In summary, these results demonstrate that the intraportal naked DNA technique could be used to produce therapeutic levels of a circulating blood protein Seπal measurements of hGH serum levels enabled the stability of expression in mdividual mice to be assessed (Table 9) In untreated ammals, hGH expression was unstable as m previous studies in which the plasmid DNA was delivered to non-hepatectomized livers usmg polylysine complexes or mtralobar injections of naked DNA An immune response could kill hepatocytes expressing the human protein To test the hypothesis that expression was unstable because of an immune response, hGH levels were followed in ammals that received cyclosponne with or without dexamethasone administration (Table 9) After an acute drop off, hGH levels remained at 6-11 ng/ml for four weeks in ammals that received both dexamethasone and cylcospoπne In animals that received dexamethasone alone or cyclosporine alone, hGH expression was prolonged as compared to the non-treated animals but not to the same extent as the animals that received both agents The ability for this gene transfer method to enable expression of a foreign gene should mcrease its utility Table 9 Mean serum levels (ng ml of serum) of human growth hormone (hGH) followmg intraportal administration of pCMVGH under optimal conditions in mice (2 to 3 ammals for each timepomt) receiving vanous treatments Optimal conditions are defined as the use of 09% salme, 15% manmtol, 2 5 units/ml hepann solution that was intraportally injected with the hepatic vem closed DAYS AFTER NONE CSA alone DEX alone CSA + DEX INJECTION 2 69 43 72 51 4 11 8 14 14 8 3 6 7 13 12 0 4 7 15 15 0 5 13 27 21 0 1 5 2 6 9 7 28 0 1 2 2 7 9 Conclusions 1 These results demonstrate that the intraportal naked DNA technique could be used to produce therapeutic levels of a circulating blood protein that is cuπently used to treat humans 2 The levels of the circulating blood protein (l e hGH) remained elevated for at least one month after a smgle mj ection Example 7 Methods After the portal veins of 25 g, 6-week old mice were exposed through a ventral midline incision, 100 μg of pBS CMVLux plasmid DNA in 0 5 ml or 1 ml of normal salme solution plus 15% manmtol and 2 5 units hepann/ml were manually injected over 30 seconds mto the portal vem near the junction of the splenic vein and portal vein The portal vein had two clamps placed distal and proximal to the point of injection so as to direct the injection fluid into only the splenic vein and to prevent the injection fluid from going to the liver or intestines The injections were done using a 30-gauge, 1/2 -inch needle and 1-ml synnge 5 X 1 mm, Kleinert-Kutz microvessel clips (Edward Week, Ine , Research Tπangle Park, NC) were used Anesthesia was obtained from intramuscular injections of 1000 μg of ketamme-HCl (Parke- Davis, Moms Plains, NJ) and methoxyflurane (Pitman-Moore, Mudelein, LL USA) which was administered by inhalation as needed was purchased from Sigma Hepaπn was purchased from LyphoMed (Chicago, LL) Two days after injection the spleens and pancreas were removed and placed m 500 ul of lysis buffer and 20 ul were analyzed for luciferase expression as descπbed above Results 28 Substantial amounts of luciferase activity were obtained in the spleen and pancreas of all four mice with both injection fluids of 0 5 ml and 1 ml Table 10. Luciferase expression after the lntravascular-administration of pBS. CMVLux into the splemc vein via the portal vein Total lucif erase/Organ (pg/organ/mouse) Injection Volume Spleen Pancreas 0.5 ml 814 4 97.2 0.5 ml 237 3 88.7 1 ml 168 7 109.4 1 ml 395 0 97.7 Mean 403 9 98.3 Standard 289 6 8.5 Deviation Conclusion 1. Intravascularly-administered plasmid DNA can express efficiently in spleen and pancreas. Example 8 Methods: 100 μg of pBS. CMVLux in 10 ml of normal saline solution plus 15% mannitol was injected into the femoral artery of adult rats with the femoral vein clamped. One to four days after injection, the quadπcep was removed and cut into 10 equal sections. Each sections were placed into 500 ul of lysis buffer and 20 ul were assayed for luciferase activity as described above. 29 Results Substantial amounts of luciferase expression were expressed in the quadπceps followmg the lntravascular delivery of plasmid DNA Table 11 Luciferase expression in the quadncep of a rat after the injection of 100 μg of pBS CMVLux into the femoral artery and with the femoral vein clamped Rat Number Total Luciferase (pg/quadnceps) 1 157 5 2 108 8 3 139 2 4 111 3 Mean 129 2 Standard Deviation 23 4 Conclusion Intravascularly-administered plasmid DNA can express efficiently in muscle Example 9 The previous examples involved injections into the afferent blood vessels of organs In the liver, the hepatic vein is an efferent blood vessel since it normally cames blood away from the liver into the infeπor vena cava Also in the liver, the portal vein and hepatic arteπes are afferent blood vessels in relation to the liver since they normally carry blood towards the liver 30 These set of experiments were designed to determine whether plasmid DNA could be efficiently expressed if delivered by a retrograde route into the efferent vessel of the liver (1 e the hepatic vein) Since another luciferase expression vector was used, pCLLuc, the results obtained with the hepatic vein injections were directK compared to results usmg the above technique of injecting the portal vein Methods 100 μg of pCLLuc in 1 ml of normal salme solution plus 15% mannitol and 2 5 umts hepann/ml were injected over 30 seconds into hepatic vein via the infeπor vena cava Smce it was difficult to directly inject the hepatic vein in rodents, the injections were directed into the infeπor cava which was clamped in two locations, proximal and distal (l e downstream and upstream) to the entry of the hepatic vein into the infeπor vena cava Specifically, the downstream infeπor vena cava clamp was placed between the diaphragm and the entry pomt of the hepatic vein The upstream infenor vena cava clamp was placed just downstream of the entry point of the renal veins Therefore, the 1 ml of the injection fluid entered the hepatic vem and the liver Smce the veins of other organs such as the renal veins enter the infeπor vena cava at this location, not all of the 1 ml of injection fluid goes into the liver In some of the ammals that received retrograde injections in the infeπor vena cava, the hepatic artery, mesentenc artery, and portal vein were clamped (occluded) for approximately five minutes immediately before and then after the injections Specifically, the order of placing the clamps were as follows first on hepatic artery, then portal vein, then downstream vena cava, and then upstream vena cava It took about three minutes to place all these clamps and then the injections were done The clamps were left in place for an additional two minutes from the time that the last clamp (upstream vena cava clamp) was placed The intraportal mjections were performed as stated using optimal intraportal injections over 30 seconds with 100 μg of pCILuc in 1 ml of normal saline solution plus 15% mannitol and 2 5 units hepann/ml and with the hepatic vein clamped for 2 mmutes Some of the mice also received daily subcutaneous injections of 1 mg/kg of dexamethasone (Elkins-Sinn, Cherry Hill, NJ) starting one day pnor to surgery 31 The pCILuc plasmid expresses a cytoplasmic luciferase from the CMV promoter. It was constructed by inserting the cytoplasmic luciferase cDNA into the pCI (Promega Coφ., Madison, WI) CMV expression vector. Specifically, a Nhel/EcoRI restπction digestion fragment containing the cytoplasmic luciferase cDNA was obtained from pSPLuc (Promega Coφ.) and inserted into pCI plasmid DNA that was digested with Nhel and EcoRI, using conventional recombinant DNA techniques (Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) in Molecular Cloning Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY). Two days after the injections, the luciferase activity was measured as above in six liver sections composed of two pieces of median lobe, two pieces of left lateral lobe, the right lateral lobe, and the caudal lobe plus a small piece of right lateral lobe. Results: A. Inferior Vena Cava/Hepatic Vein Injections with the Portal Vein and Hepatic Artery Clamped (*Injections in animal #3 were not optimal since the fluid leaked during the injections.) Injections were done in 6-week old animals that received dexamethasone. Luciferase Activity (ng) Sections Animal #1 Animal #2 Animal #3* 1 5,576.7 4,326.4 1,527.4 2 8,511.4 4,604.2 1,531.6 3 5,991.3 5,566.1 2,121.5 4 6,530.4 9,349.8 1,806.3 5 8,977.2 4,260.1 484.2 6 9,668.6 6,100.2 1,139.3 total liver 45,255.5 34,206.9 8,610.4 Mean 29,357.6 standard deviation 18,797.7 B. Inferior Vena Cava/Hepatic Vein Injections with the Portal Vein and Hepatic Artery not Clamped. Injections were done in 6-week old animals that did not receive dexamethasone. Luciferase Activity (ng) 32 Sections Ammal #1 Ammal #2 1 3606 5062 2 4135 7247 3 4630 6260 4 5155 7586 5 3516 6648 6 4378 7496 total liver 2,542 0 4,029 8 Mean 3,285 9 standard deviation 1,052 1 C. Portal Vem Injections with the Hepatic Vein Clamped in 6 month old mice that received dexamethasone Luciferase Activity (ng) Sections Ammal #1 Ammal #2 Ammal #3 i 2874 4170 129.2 2 6337 8081 2205 3 6898 1,0965 328.2 4 9578 1,0569 1816 5 6607 1,4874 1786 6 8124 1,2764 2334 total liver 4,041 8 6, 142 2 1,271 5 Mean 3,818 5 standard deviation 2,443 0 33 D. Portal Vein Injections with the Hepatic Vein Clamped in 6 week old mice that received dexamethasone Luciferase Activity (ng) Sections Ammal #1 Ammal #2 Ammal #3 _ 352 9 379 1 87 0 2 667 5 373 9 108 2 3 424 8 1,277 9 178 4 4 496 3 1 308 6 111 9 5 375 2 296 4 162 3 434 7 628 7 123 0 total liver 2.751 4 4,264 7 770 9 Mean 2,595 7 standard deviation 1,752 1 E Summary Table Compaπng the Luciferase Expression Obtained Using the Above Conditions Injection Condition® Mean Total Luciferase/Liver Times (μg/hver) Condition D ___ Condition A 29 4 Condition B 3 3 1 27 X Condition C 3 8 1 46 X Condition D 2 6 1 00 X Condition A= Infeπor Vena Cava/Hepatic Vein Injections with the Portal Vem and Hepatic Artery Clamped in 6 week-old animals that received dexamethasone Condition B= Infenor Vena Cava/Hepatic Vein Injections with the Portal Vein and Hepatic Artery not Clamped in 6-week old animals that did not receive dexamethasone Condition C= Portal Vein Injections with the Hepatic Vein Clamped in 24 week old mice that received dexamethasone 34 Condition D= Portal Vein Injections with the Hepatic Vein Clamped in 6 week old mice that received dexamethasone Conclusions 1 Retrograde delivery of plasmid DNA into the efferent vessels of the liver via the hepatic vein/mfenor vena cava leads to high levels of gene expression 2 The highest levels were achieved usmg this retrograde approach if the afferent vessels to the liver (portal vein and hepatic artery) were occluded 3 The CLLuc plasmid enabled much higher levels of luciferase expression than the pBS CMVLux plasmid (see above examples) using the portal vein approach m both 6-week old and 6-month old mice 4 Under all conditions, luciferase expression was evenly distnbuted throughout all six liver sections Example 10 Ammals that received injections into the inferior vena cava were assayed for luciferase to determine whether retrograde delivery into the efferent vessels (veins) of other organs enable gene expression Methods In the same animals that were injected using condition A above (Infenor Vena Cava/Hepatic Vein Injections with the Portal Vein and Hepatic Artery Clamped in 6 week-old ammals that received dexamethasone) the kidneys were removed and assayed for luciferase as described above In the same animals that were injected under condition B above (Infenor Vena Cava/Hepatic Vein Injections with the Portal Vein and Hepatic Artery NOT Clamped in 6- 35 week old animals that did not receive dexamethasone), the adrenal gland and diaphragm muscle, abdominal muscles, and back muscles were removed for luciferase analysis. Results: A. Luciferase Activity in Kidneys in Animals Injected Under Condition A. Total Luciferase Activity Kidney (pg/kidney) Animal #1 Animal #2 Animal #3* Right Kidney 10,827.8 7,662.3 636.3 Left Kidney 733.1 753.8 479.7 * Injection fluid leaked. B. Luciferase Activity in Adrenals and Various Muscles Injected Under Condition B. Total Luciferase Activity /Tissue (pg/tissue) Animal #7 Ammal #8 Animal #9 right adrenal not assayed 82.0 49.9 left adrenal not assayed 48.4 42.2 Diaphragm 41.9 67.9 117.6 Abdomen 40.4 43.9 44.0 Back 37.7 40.1 40.9 Conclusions: 1. Retrograde delivery of plasmid DNA into the efferent vessels of several different tissues led to substantial levels of foreign gene expression in the tissues. 2. These tissues include the adrenal glands (suprarenal glands), the diaphragm muscle, back muscles and abdominal muscles. 3. Foreign gene expression in the diaphragm would be especially useful for Duchennes muscular dystrophy since humans with this disorder die from respiratory failure due to fibrosis of the diaphragm muscle 36 Example 11 This example explores the use of more accessible vessels such as the hepatic vein and the bile duct, for delivering the naked pDNA m mice. Efficient gene expression was obtained using these efferent delivery routes. Occlusion of other vessels to restπct outflow of the injection solutions enhanced but was not critical for efficient expression. Repetitive injections into the bile duct were also accomplished Preliminary results are also presented in larger animals, the rat and dog The incoφoration of these findings into laboratory and clinical protocols is discussed Materials and Methods: Plasmid Constructs The pCILuc plasmid expresses a cytoplasmic luciferase from the human CMV immediately early (hCMV ED) promoter. It was constructed by inserting the luc+ gene, an Nhe I- EcoR I luc+ fragment from pSLuc+ (Promega, Madison, WI), into the pCI expression vector (Promega). pCILux expresses peroxisomal luciferase under control of the hCMV LE promoter It was constructed by inserting the luciferase gene (Hind HI - Bam HI fragment from pBlueCMVLux) into the Sma I site of pCI pCLLacZ was constructed by placing the E. coli LacZ gene (Pst I - Apa I fragment pBS-RSV-LacZ) into the pCI vector (Sma I site). The pCMVGH construct was previously descπbed (Andree, et al., 1994). Injection Methods Plasmid delivery into the hepatic vessels was performed in 6-week old ICR mice, 2.5-6.25 month-old, 200-300 gram Sprague-Dawley rats, and beagle dogs. Ventral midhne incisions were performed to expose the liver and the associated vessels. The mice were anesthetized with intramuscular injections of 1000 μg of ketamine-HCl (Parke-Davis, Moms Plams, NJ) and methoxyflurane (Pitman-Moore, Mudelein, IL) which was administered by inhalation as needed. The rats were anesthetized with ether and the dogs were anesthetized with halothane by inhalation The pDNA was injected in solutions containing 2.5 units/ml or hepaπn (Lypho-Med, Inc., Chicago, IL) (Qian et al., 1991) and either normal saline (0 9% NaCl) or 15% manmtol in normal saline (Sigma Chemical Co., St. Louis, MO). All animals received humane care in compliance with institutional (TACUC) guidelines. 37 In mice, the intraportal injections were performed as previously described (Budker, et al., 1996). 100 μg of pDNA in 1 ml were manually injected over -30 see using a 30-gauge, 1/2-inch needle and 1-ml syringe without occluding the portal vein upstream from the point of injection. In some animals, a 5 X 1 mm, Kleinert-Kutz microvessel clip (Edward Week, Inc., Research Triangle Park, NC) was applied during the injection at the junction of the hepatic vein and caudal vent cava. DNA was delivered in mice to the hepatic vein via an occluded IVC Clamps (6 X 1 mm, Kleinert-Kutz curved microvessel clip (Edward Week, Ine . Research Triangle Park, NC) were applied downstream (toward the heart) of the hepatic vein and upstream (towards the legs) of the hepatic and renal veins. Injections were done upstream of the hepatic vein. In some of the injections, the portal vein and hepatic artery were clamped using 6 X 1 mm, Kleinert-Kutz curved microvessel clips. The IVC mouse injections were also performed with 100 μg of pDNA in 1 ml that were manually injected over -30 sec with a 30-gauge, 1 /2-inch needle and 1-ml syringe. The bile duct injections in mice were performed using manual injections with a 30-gauge, 1/2-inch needle and 1-ml syringe. A 5 X 1 mm, Kleinert-Kutz microvessel clip was used to occlude the bile duct downstream from the point of injection in order to prevent flow to the duodenum and away from the liver. The gallbladder inlet was not occluded. In some of the bile duct injections, the junction of the hepatic vein and caudal vena cava clamped as above. In yet other injections, the portal vein and hepatic artery were clamped in addition to the occlusion of the hepatic vein. In mice, repetitive injections into the bile duct were done by placing a polyethylene tube (I.D. 0.28 mm, O.D. 0.61 mm, Intramedic Clay Adams Brand. Becton Dickinson Co., Sparks, MD, USA) catheter into the bile duct after making a hole with a 27 gauge needle. The tubing was secured by a suture around the bile duct and tubing; thereby occluding the bite duct. The other end of the tubing was placed outside the skin of the animal's back so that surgery was not required for repeat injections. No blood vessel occlusions were done for these repetitive administrations. After completion of the studies, anatomical examination indicated that the catheter remained in the bile duct. 38 In rats, the intraportal, IVC, and bile duct injections were done as in mice but with the followmg modifications The injections were done through a 25 -g butterfly needle using a peπstaltic pump (Preston vaπstaltic power pump, Manostat Coφ , New York, NY) over 1 or 3 mmutes The downstream IVC clamps in the IVC injections were done downstream of the kidneys For the portal vein injections the portal vein and hepatic artery were clamped The outflow through the hepatic vein was restπcted in some animals by clamping the upstream and downstream TVC In some ammals the livers were first flushed with normal saline pnor to DNA injection Rat bile duct injections were done the same as mice The rat doesn't have a gallbladder In some of the rat portal vein injections, a 25 G needle connected to a pressure gauge (Gilson Medical Electronics, Model ICT-1 1 Lnigraph), was inserted into the liver parenchyma to determine the peak pressure within the liver dunng the injections The statistical relationship between pressure and luciferase was done using Spearman's rank coπelation A smoothmg splme regression model descπbing the relationship between log luciferase and pressure was estimated using generalized additive model methodologv (Hastie, 1992) Akaike's information cπteπa (Akaike, 1973) indicated that a smoothing spline with 2 degrees of freedom resulted m the best fit over all integer degrees of freedom between 0 and 5 The mjections in dogs were done as in rats except that an 18 gauge, 2 inch angiocath (Becton Dickinson, San Jose, CA) was used All dogs except dog #1 were females Table I indicates the injection conditions For the bile duct injections, a suture was applied to occlude transiently the bile duct downstream from the point of injection A DeBakey multipurpose vascular clamp was applied to the cystic duct dunng injection to prevent the injectate from entering the gallbladder In dogs, the DNA was pCILux Protein Assays The luciferase assays were done as pre\ lously reported (Wolff, et al , 1990) One day after pCILuc injections, the animals were sacrificed and the rodent livers were divided mto 6 sections composed of nght lateral lobe, caudate lobe two pieces of median lobe and two pieces of left lateral lobe For each of the six pieces 0 7 ml of lvsis buffer (0 1% Tπton X-100, O IM potassium phosphate, 1 mM DTF pH 7 8) was used for mice and four ml of lysis 39 buffer were used for rat liver For the dog livers, approximately 10% of each lobe was divided mto five to 20 pieces and placed into two ml of lysis buffer The samples were homogenized usmg a PRO 200 homogemzer (PRO Scientific Inc., Monroe, CT) and centπfuged at 4,000 φm for 10 min at 4°C Twenty μl of the supernatant was analyzed for luciferase activity. Relative light units (RLU) were converted to pg of luciferase using standards from Analytic Luminescence Laboratoπes (ALL, San Diego, CA) Luciferase protein (pg) = 5.1 X 10 "5 X RLU+ 3.683 (r = 0 992) Ten-μm thick tissue sections were stained for β-galactosidase expression as previously described usmg 1-4 hour X-GAL incubations (Budker. et al , 1996) Hematoxylin was used for the counterstain but the alkaline step was omitted so that the hematoxylm stain remained red. The percent of blue-stained cells in the liver sections was determined by counting ~3000 cells in three sections and averaging Blood obtained from the retro-orbit sinus was analyzed for serum concentration of hGH using the RIA, HGH-TGES 100T kit from Nichols Institute (San Juan Capistrano, CA). Serum ALT and GGT levels were done usmg EKTACHEM DT slides and a KODAK EKTACHEM DT 60 ANALYZER as recommended by the manufacturer (Kodak, Rochester, NY) Mice Luciferase Experiments Our previous studies used the pBS CMVLux plasmid for evaluating the optimal conditions for naked pDNA expression following lmportal injection. These optimal conditions were intraportal injections of 100 μg of pDNA m 1 ml of 15% manmtol and 2 5 units hepann/ml in normal saline solution The injections were done over 30 seconds with the hepatic vein and IVC occluded In this study, 100 μg of pCILuc injected under similar conditions yielded a mean total luciferase protein/liver of 3 73 μg/liver (Fig. 1 A, PV+CL), approximately 30- times greater than that obtained with pBS CMVLux. Part of this increase could be attributed to greatei operator experience with these injection techniques. Injections with pCILuc under these conditions without clamping the hepatic vein yielded approximately 750-fold less luciferase (Fig 1A, PV-CL) The hepatic veins (via the IVC) of another set of mice were injected with 100 μg of pCILuc in 1 ml of 15% mannitol and 2 5 units hepann/ml in normal saline solution A mean total 40 luciferase protein/liver of 17.34 μg/liver (Fig. 1A, FVC+CL) was obtained when the portal vein was clamped as compared to a mean total luciferase protein/liver of 2.83 μg/liver (Fig. 1 A, IVC-CL) without occluding the portal vein. Similar results were also obtained when bile ducts were injected with 100 μg of pCILuc in 1 ml of 15%) mannitol and 2.5 units heparin/ml in normal saline solution. A mean total luciferase protein/liver of 15.39 μg/liver (Fig. 1 A. BD+CL) was obtained when the hepatic vein was clamped as compared to a mean total luciferase protein/liver of 1.33 μg/liver (Fig. 1A, BD-CL) without occluding the hepatic vein. If mannitol was omitted then the bile duct injections without clamping any blood vessels yielded approximately 15-fold less luciferase (0.086 μg/liver + 0.06, n = 25). Clamping the hepatic artery, and portal vein in addition to the hepatic vein did not improve expression beyond what was obtained when only the hepatic vein was clamped (data not shown). Serum ALT and GGT assays were performed on mice one and eight days after each of the above injections with pCILuc (4 mice for each condition). No increases in GOT were observed after any of the injections including the bile duct injections. Serum ALT levels increased to 200-400 U L one day after portal vein and bile duct injections. One day after IVC injections serum ALT levels increased to - 1500 U/L in half of the mice but was only ~250 U/L in the other half. By eight days after injection, serum ALT levels decreased to baseline levels in all animals. For positive control puφoses, a non-lethal intraperitoneal injection of 40 μl of 50%, carbon tetrachloride in mineral oil was performed. An average of 25,900 U/L (n=4) was observed one day after injection. Rat and Dog Luciferase Experiments: Similar injections into the portal vein, IVC (to the hepatic vein), and bile duct were done in rats (Fig. IB). For the portal vein injections, the injection volumes were increased 15-times over that used in mice because the rat livers are ~15-times larger than mouse livers. One day after 750 of μg of pCILuc in 15 ml of 15% mannitol and 2.5 units heparin/ml in normal saline solution were injected into the portal vein while occluding the hepatic vein, an average of 53.5 μg of luciferase/liver was obtained (Fig. IB, PV+CL). The efficiency of gene transfer in the rat was compared to that in mice two ways. In terms of efficiency as defined by the mg of luciferase per mg of tissue weight, the levels of expression for portal injections were 3.6 μg of luciferase/g of tissue in rats as 41 compared to 3 7 μg of luciferase/g of tissue in mice Alternatively, in terms of efficiency as defined by the ng of luciferase per μg of pDNA delivered, the efficiencies of expression for portal injections were 71 ng of luciferase/μg DNA in rats as compared to 37 ng of luciferase/μg DNA in mice Less but still substantial luciferase expression was obtained when the injections of pCILuc were done into the efferent vessels of rats such as the TVC or bile duct (Fig IB) Injections of 750 μg of pCILuc in 15 ml into the hepatic vein (via the IVC) while occluding the portal vem yielded an average of 1 5 μg of luciferase/liver Injections of 750 μg of pCILuc in 5 to 8 ml mto the bile duct without any outflow obstruction yielded an average of 1 3 μg of luciferase/liver Parenchymal pressures of 12-50 mm Hg were measured in 23 rat livers dunng the mj ection of 750 μg pCILuc m 15 or 20 ml into the portal vein while occluding the TVC (Fig 2) Spearman's rank coπelation between pressure and luciferase expression was 0 76 (two-side p-value < 0 001) indi eating that pressure and luciferase were sigmficantiy positively associated It appeared that pressures of over 40 mm Hg did not result in increased expression The necessity of the non-linear component over and above a simple linear fit was verified by an approximate full versus reduced F-test (p-value = 0 0 14), indicating that the observed plateau effect is real Examination of both the residual quantile-quantile and the residual versus prediction plots further reveals that there are no senous violations of the regression model assumptions and therefore the regression models and p-values are valid (Fisher and van Belle, 1993) Preliminary expeπments explored the ability of naked pCILux (not pCILuc) to be expressed m dogs (Table I) In mice, in the case of intraportal injection, pCILux provided 3x lower expression than pCILuc (data not shown) In five dogs, vaπous amounts of DNA were mjected into either the bile duct with or without blocking outflow by occluding the IVC In one ammal, the DNA was injected into the TVC without any outflow blockage All the dogs survived the procedure except animals that had the IVC occluded recovered more slowly post-operatively The animals were sacrificed one day after the injections and dozens of tissue samples from each liver lobe were analyzed for luciferase The luciferase expression was 42 evenly distributed over all the lobes in each liver except in one lobe of one dog. Routine histologjcal analysis in dog #5 (Table 1) indicated that the tissue architecture was substantially disrupted suggesting that the injection volumes were too large. Decreasing the volume of injection in dog #6 to 200 ml resulted in the best expression (Table I)- Mice and Rat β-Galactosidase Results The β-galactosidase expression vector was used to determine the percent and type of cells that were transfected (Fig 3). As previously noted for portal vein injections (Budker, et al., 1996), the vast majoπty of the blue-stained cells appeared to be hepatocytes on moφhological grounds but a few appeared to be endothelial or other types of cells A preponderance of hepatocytes were also stained blue after the bile duct or IVC injections in mice or rats (Fig 3 ) Under the vaπous inj ection conditions, the percent of cells paralleled the levels of luciferase expression (Figs 1 and 3) In mice, the IVC injections gave the highest percentage of β-galactosidase-positive cells in which 7% of hepatocytes were positive (Fig. 3B). In rats, the portal vein injections gave the highest percentage of β-galactosidase-positive cells in which 7% of hepatocytes were positive (Fig 3C) In some animals, liver cell damage was evident in less than 5% of the cells (for example in Fig. 3A). Of note in the rat livers injected into the portal vein, almost all of the positively-stained cells were penacinar with few positive cells around the central vein (Fig 3C). Repeat Bile Duct Iniections- The bile ducts of mice were cannulated and 100 μg of pCMVhGH in 1 ml of 15% mannitol in normal saline were injected once a week (Fig. 4). Serum levels of hGH increased one day after the fast injection and then decreased to background levels by seven days after injection. One day after the second injection, hGH levels again increased and then were back to background levels by seven days after the second injection. Only minimal increases in hGH levels occuπed after the third injection. Mice that had the highest levels after the first injection had the lowest levels after the second injection (mice 3 and 6) and vice versa (mice 1, 2. and 4). In another set of animals (4 mice), the bile duct injections were repeated four times with pCMVhGH and then pCILuc was injected. The first three pCMVhGH injections led to similar increases in hGH serum levels as in Fig. 4. 43 Although there were only minimal raises in hGH serum levels following the fourth injection, injection of pCILuc yielded an average of 29.2 ng/liver (± 7.1 , n=3). The liver in one of the four mice was grossly yellow and scarred as a result of the bile duct ligation and did not express any luciferase. Discussion This report extends the findings of the previous study showing pDNA expression following afferent intraportal delivery and demonstrates efficient plasmid expression following delivery via efferent vessels such as the hepatic vein or bile duct. Expression of luciferase or β- galactosidase was evenly distributed throughout the entire liver when either of the three vessels were injected. Combining these surgical approaches with improved plasmid vectors enabled uncommonly high levels of foreign gene expression in which over 15 μg of luciferase protein/liver was produced in mice and over 50 μg m rats (Fig. 1 and 2). Equally high levels of β-galactosidase expression were obtained in that 7% of the hepatocytes had intense blue staining with only a one hour X-GAL incubation (Fig. 2). These levels of foreign gene expression are among the highest levels obtained with nonvital vectors and approach what can be achieved with vital vectors. Using the portal vein administration route, occlusion of the outflow is critical for expression. Outflow occlusion increases the expression with the efferent administrative routes, but substantial amounts of expression were obtained even when the hepatic vein was not blocked. Most likely the natural direction of blood flow provides a sufficient impetus to retard the egress of injection fluid and raise the hydrostatic pressure. The use of these efferent vessels simplifies the admmistiation for potential human applications since they are easier to access by non-invasive methods. If no occlusion is used then only one vessel has to be reached. These efferent routes should also be considered for the administration of viral and non-viral vectors as has been done with the delivery of adenoviral vectors into the bile duct [Vrancken Peeters, et al., 1996b; Vrancken Peeters, et al., 1996a; Yang et al., 1993). The mechanism of pDNA uptake is not known but may involve native cellular uptake processes (Budker, et al., 1996) It is of interest that high levels of luciferase expression could 44 occasionally be obtained when the DNA was injected into the bile duct in small volumes of isotonic solutions without occluding the IVC Increased osmolar and hydrostatic pressure may not be cntical for uptake of the pDNA by hepatocytes as they are not in muscle cells (Wolff, et al., 1990, Wolff, et al., 1991. Wolff, et al., 1992a). This would suggest that the mechanism of pDNA uptake may in fact involve endogenous cellular pathways. Increased hydrostatic and osmotic pressures may raise expression by enhancing these cellular internalization processes (Haussinger, 1996). For example, hepatocyte shrinkage stimulates replication of the duck hepatitis B virus (Offensperger, et al., 1994). The raised pressures could also increase the delivery of the pDNA to the hepatocyte surface not only for the blood vessel administrations (through the sinusoidal fenestrae) but for the bile duct injections as well The increased pressures could transiently attenuate bile secretion thereby decreasing the clearance of the pDNA (Stieger, et al., 1994). For example, the hyperosmolar mannitol should induce cell shrinkage that is known to inhibit taurocholate excretion into bile (Haussinger, et al , 1992) Hepatocytes are functionally polanzed cells m which the basal and apical membranes have different exocytic, endocytic and transcytotic functions (Hubbard, et al., 1994). The success with either blood vessel or bile duct routes could indicate that both the basal (sinusoidal) and apical (bile canalicular) membranes share a common pathway for the cellular entry of pDNA. However, the passage of DNA between the basal -lateral and bile canalicular spaces cannot be excluded even under the more gentle injection conditions. Plasmid DNA injected into the bile duct could be taken up from the basal-lateral surface of hepatocytes after passage pancellularly. If only the basal-lateral surface takes up the pDNA then the increased hydrostatic and osmotic pressures may enhance pDNA expression by disrupting the tight junctions between hepatocytes and thereby increase the flow of pDNA between the canalicular and basal-lateral spaces (Desmet and De Vos, 1982). The bile duct was cannulated to determine whether repeat injections could be done. Substantial hGH levels were obtained alter the first two injections (Fig. 3). The hGH levels dropped considerably by one week after each injection presumably because of an immune response to the foreign protein The inability to detect hGH after the third or fourth injections 45 was most likely due to a more rapid response of the immune response sensitized to hGH. The ability to subsequently obtain luciferase expression argues against an immune response against the pDNA that prevents expression Previous studies have failed to detect anti-DNA antibodies following the administration of naked pDNA (Nabel, et al., 1992; Jiao, et al., 1992). The ability to repetitively administer naked pDNA without inducing an immune response against the vector is a distinct advantage of naked pDNA over viral and some types of non-viral vectors Other studies in progress in our laboratory suggest that suppression of the immune system enables more persistent expression In post-mitotic myofibers, plasmids can persist extrachromosomally and express for at least two years; presumably because the pDNA is not being lost as result of cell division (Wolff, et al., 1992b; Herweijer, et al., 1995). Quite possibly pDNA would be lost slowly in hepatocytes which have a half-life of up to a year in rodents and humans (Webber, et al , 1994, Leffert, et al., 1988). If so then liver-based genetic disorders such as hemophilia could be treated by injections every six months. The bile duct could be accessed repeatedly by upper gastrointestinal endoscopy Similarly, the hepatic vein could be non-invasively accessed via peripheral or central veins. In addition, gene transfer could be delivered to newborns via the umbilical cord vessels to get them over a newborn metabolic cπsis as occurs in the organic acidunas and the urea cycle defects. Often gene transfer techmques that work in mice do not work in larger animals. Our results demonstrate that the technique works in rats that are approximately ten-fold bigger than mice. The dog results indicate that the liver of larger non-rodent mammal can express naked pDNA. Although substantial levels of luciferase activity were obtained, further optimization of the injection conchdons is required to increase the efficiency of expression so that they are comparable to those in rodents The studies to determine the relationship between intraparenchymal pressure and luciferase expression in rats are a first step towards this goal. Minimal liver cell damage occuπed in the rodents as evident by serum chemistries and histology but the injections were more disruptive to the hepatocytes in dogs. Presumably, the key factor is the efficient delivery of the pDNA to the hepatocyte surface with minimal cellular or tissue disruption (Fig. 2) 46 In the research laboratory, the descnbed techniques will enable rodents to be used just as immortalized and pnmary liver cell cultures are now used for gene and cellular studies of liver function. The transfer of genes into cells in culture have been a critically important tool for deciphenng the function of genes and for studying the effect of expressed proteins on cellular processes. Typically, the gene under study is placed within a plasmid vector and transiently transfected mto the appropriate cell in culture. Iso forms and mutant forms of the gene under study can be quickly placed mto plasmid expression vectors and studied. Our findings indicate that a similar plasmid-based approach could be used to study the effects of gene function in hepatocytes in sun Given that the high levels of expression are transient in this system, it would be best if these effects occurred within a few days. The use of pDNA avoids the laborious steps necessary for the production of vital vectors or generation of transgenic mice and thereby enables many different genes and their related mutated forms to be quickly studied. It will permit are mechanism of gene expression and their effects on liver function to be expeditiously probed within the context of a complete mammalian organism. Example 12 The lntravascular delivery of naked pDNA to muscle cells also has attraction. Muscle has a high density of capillaπes (Browning, 1996) and the capillanes are in close contact with the myofibers (Lee 1995) However, the endothelium in muscle capillaries is of the continuous, non-fenestrated type and has low solute permeability especially to large macromolecules(Taylor,A.E.,and D N. Granger. Exchange of macromolecules across the microcirculation In Handbook of Phusiology The Cardiovascular System. Microcirculation Bethesda, MD Am. Physiol.Soc , 1984, sect. 2, vol. IV, chapt. 11, p. 467) The mechanism of macromolecule transendothehal transport is poorly understood. Cell biologists have proposed that this transport occurs by transcytosis involving plasmalemmal vesicles or convective transport through transient transendothelial channels formed by the fusion of vesicles (Michel C C Cardiovascular Res. 1996). Physiologists have modeled the muscle endothelium as havmg a large number of small pore with radii of 4 nm and a very low number of large pores with radu of 20-30 nm (Rippe B. Physiological Rev, 1994). Although the radius of gyration of 6 kb pDNA is -100 nm (Fishman, D.M , Biopolymers, 38, 535-552), supercoiled DNA in plectonomic form has superhehx dimensions of approximately 10 nm 47 (Rybenkov V.V. J. Mol. Biol. 267, 299-311 1997). This implies that pDNA has a possibility to traverse microvascular walls by stringing through their large pores. We hypothesized that the rate of pDNA extravasation could be increased by enhancing fluid convection through these large pores by raising the transmural pressure difference in selective regions. This report demonstrates that intravascular pDNA injections under high pressure can in fact lead to high levels of foreign gene expression in muscles throughout the selected hindlimb of an adult rat. Hydrostatic Pressure 475 ug of pCILux in normal saline solution (NSS) was injected into the femoral arteries of adult Sprague-Dawley rats while blood inflow and outflow were blocked. Injection of pCILux, a luciferase expression vector utilizing the CMV promoter (LIVER U promoter accepted for HGT), was done after both the femoral artery and vein was occluded for 10 min. Two days after pDNA injections, the luciferase activities were measured in all the muscles of the hindlimb (Fig. 5). The highest levels of luciferase expression were achieved when the pCILux was injected in 9.5 ml of normal saline within 10 sec. Injection volumes less than 9.5 ml resulted in substantially less expression (Fig. 5(a)). Injection times more than 10 sec also resulted in much less expression (Fig. 5(b)). This critical dependence on volume and speed of injection indicates that either increased hydrostatic pressure or rapid flow is required for efficient expression. Artery injections performed without occluding the femoral vein resulted in approximately 200-fold less expression (1 8±1.2 ng of luciferase per all hindlimb muscles). Other Factors Further studies were performed to determine the effect of ischemia on the expression of the intravascularly injected pCILux. The time of ischemia was adjusted by varying the time that both the femoral artery and vein were occluded prior to pDNA injection (Fig. 5(c)). Although the highest level of expression was obtained with 10 min of ischemia, the expression levels were only a few-fold lower at shorter or longer ischemia times. This suggests that ischemia is not a critical factor for enabling egress of the pDNA out of the intravascular space. However, the blood flow for the zero ischemia time point is disrupted for -30 sec and this may be sufficient to affect vascular permeability. Ischemia could increase expression either by capillary recruitment and vasodilatation) or augmenting permeability (Mathieu-Costello O. Int J crocirc 1995.15, 231-237). In addition, ischemia could possibly increase pDNA expression by affecting transcription or translation. Ischemia can be tolerated by muscle for 48 two to three hours (Gidlof A , Lnt J Microcirc Chn Exp 1987, 7,67-86) Histologic analysis of the muscle did not reveal any hemoπhage or myofiber damage Other factors were explored to increase the level of expression Hypotonic (Fig 6, condition 2) or hypertonic (Fig 6, condition 3) injection solutions resulted m less expression Although, the effect of the hypertonic mjection solution ( normal salme solution with 15%) mannitol) may have been mitigated by the slower rate of injection (over 30 sec instead of 10 sec) that results from its increased viscosity The pre-inj ection with collagenase resulted in a 3 5 -fold increase to 1,332 ng/total muscles (Fig 6, condition 4) The collagenase was used to disrupt the basement membrane of the capillaries and thereby increase pDNA egress It could have also mcreased expression by disrupting the extracellular matnx withm the muscle Further studies are m progress to determine the optimal conditions for collagenase administration without causing hemoπhage Distπbution of Foreign Gene Expression The luciferase expression was distributed among all muscle groups in the leg (Table 1) The lower levels in the lower anterior leg and foot are probably due to their high content of tendons and small amount of muscles The levels after intravascular injection was up to 40 times higher than the levels after intramuscular mjection The type and percentage of the transfected cells in the muscle were determined usmg the β-galactosidase reporter system Using the best injection condition (condition 4 in Fig. 6), approximately 10-50% of the myofibers expressed β-galactosidase These expression results provide indirect evidence that pDNA extravasation occurred More direct evidence was obtained usmg fluorescently-labeled DNA mjected mto the femoral artery (condition 1 in Fig 6) The labeled DNA was distπbuted extravascularly in all the limb muscles and surrounded most of the myofibers (data not shown) Conclusion These results demonstrate that the intraarteπal delivery of pDNA to muscle can be greatly enhanced when injected rapidly, in a large volume and when all blood vessels leading mto and out of the hindlimb are occluded These conditions presumably increase the intravascular hydrostatic pressure and therebv increase the convective outflow that transports the pDNA into contact with myofibers The high intravascular pressure may increase the 49 number, size and permeability of the microvascular pores (Rippe, B. Acta Physiol. Scand. 125, 453-459, 1985) (Wolf, M.B Am J Physiol 257, H2025-H2032,1989) . Preliminary studies using collagenase suggest that enzymatic disruption of the vessels basement membrane or muscle extracellular matrix may also increase the delivery of pDNA to the myofibers. Ischemia also increased expression moderately Furthermore, it is expected that other enzymes such as hylaronidase will perform similarly to collagenase. This study shows that the intravascular approach increases expression in muscle up to 40-fold as compared to intramuscular injection This is the first demonstration that naked plasmid DNA can be efficiently expressed in muscles of adult animals larger than mice. It is also the first report of an intravascular non- viral gene transfer approach for muscle. Further studies in larger animals will determine the clinical relevance of this study. Description of Injections A 4 cm long abdominal midhne excision was performed in 150-200 g, adult Sprague- Dawley rats anesthetized with 80 mg/mg ketamme and 40 mg/kg xylazine. Microvessel clips (Edward Week, Ine , Research Triangle Park, NC) were placed on external iliac, caudal epigastric, internal iliac and deferent duct arteries and veins to block both outflow and inflow of the blood to the leg A 27 g butterfly needle was inserted into the external iliac artery and the DNA solution was injected by hand. Table 1. Distπbution of luciferase expression among the different hindlimb muscle groups two days following lntraarterial injection with 475 μg of pCILux m 9 5 ml of NSS over 10 Means and their standard deviations are shown for 6 animals Injection type Muscle group Total Luciferase Luciferase Cone. (ng) (ng/g tissue) Intravascular Upper Leg Antenoi 149. 51.2 109.2 ± 37 5 Upper Leg Posterior 74.6 ± 36.0 43.4 ± 20 9 Upper Leg Medial 88.7 ± 63.7 61.6 ± 44.5 Lower Leg Postenor 114.5 ± 89.7 66 0 ± 51 7 Lower Leg Anterior 3 0 ± 1.0 5.4 ± 1.7 50 Foot 0 5 ± 0 2 4 1 ± 1 4 Intramuscular Upper Leg Antenor 3 7 ± 0 9 2 8 ± 0 8 Example 13 Adenoviral vectors can be delivered to muscle parenchymal cells by an intravascular route. Methods' the same methods that were used to deliver naked plasmid DNA to muscle in rats were also used The adenoviral vector CMVLacZ that expresses the E. coli beta-galactosidase from the immediate early promoter of the human cytomegalovirus (commonly called the "CMV promoter") was prepared as previously descπbed (Yang, T., K U Jooss, Q. Su, HC. J. Ertl and J M Wilson Immune responses to viral antigens versus transgene product in the elimination of recombinant adenovirus-mfected hepatocytes in vivo. Gene Therapy, 3(2): 137- 144, 1996) The rat iliac artery was preinjected with 0.5 mg of papavenne and 40 ng of collagenase in 3 ml of saline while blockmg the iliac artery and vem 5 X 10 8 particles of the adenoviral vector CMVLacZ in 10 ml of salme was injected m about 10 sec and 2 mmutes later the clamps were opened Two days after injection the leg muscle were assayed for luciferase as above Results- Table: Distribution of luciferase activity following the intraarteπal injection of adenovirus CMVLacZ Muscle Group Luciferase (ng) Upper Leg Anterior 59.04 Upper Leg Posterior 18.33 Upper Leg Medial 4.44 Lower Leg Posterior 11.04 Lower Leg Anterior 5 33 Foot 0.22 Total === ===== === 98 40 51 The foregoing is considered as illustrative only of the pnnciples of the invention. Further, since numerous modifications and changes will readily occur to those skilled in the art, it is not desired to limit the invention to the exact construction and operation shown and described. Therefore, accordingly, all suitable modifications and equivalents fall within the scope of the invention 52

Claims: We Claim: 1. A process for dehvenng a polynucleotide to a parenchymal cell, compnsing: inserting the polynucleotide into a vessel for delivery to the cell such that the polynucleotide is transfected into the cell 2. The process of claim 1 wherein the polynucleotide is selected from the group consisting of natural RNA, DNA and non-natural polynucleotides 3. The process of claim 2 wherem the polynucleotide is a viral vector 4. The process of claim 3 wherein the polynucleotide is a vector selected from the group consisting of adenoviral, adenoassociated viral and retroviral. 5. The process of claim 1 wherein the vessel consists of a blood vessel having a permeable wall 6. The process of claim 5 wherein the blood vessel is selected from the group consisting of afferent and efferent vessels 7. The process of claim 7 wherem the permeability is increased by a method selected from the group consisting of increasing hydrostatic pressure on the blood vessel wall, increasing osmotic pressure on the blood vessel wall, and introducing a biologically- active molecule to the blood vessel wall. 8. The process of claim 8 wherein the hydrostatic pressure is increased by obstructing outflow from the blood vessel 53 9. A process for dehvenng a polynucleotide to a cell, comprising: a) forming the polynucleotide for binding with a molecule selected from the group consisting of RNA, DNA, nucleic acid hybrids, derivatives of natural nucleotides and protein; and, b) inserting the polynucleotide into a vessel for delivery to the cell. 10. The process of claim 9 wherein the polynucleotide comprises a natural or non-natural polynucleic acid for preventing expression. 11. The process of claim 10 wherein the polynucleotide comprises anti-sense. 12. The process of claim 11 wherein the vessel consists of a blood vessel having a permeable wall 13. The process of claim 12 wherein the blood vessel is selected from the group consisting of afferent and efferent vessels. 14. The process of claim 13 wherein permeability is increased by a method selected from the group consisting of increasing hydrostatic pressure on the blood vessel wall, increasing osmotic pressure on the blood vessel wall, and introducing a biologically- active molecule to the blood vessel wall. 15. The process of claim 14 wherein the hydrostatic pressure is increased by obstructing outflow from the blood vessel. 16. A process for delivering a polynucleotide to a cell, comprising: a) forming the polynucleotide to recombine with a molecule selected from the group consisting of RNA and DNA, nucleic acid hybrids, and derivatives of natural nucleotides; and, b) inserting the polynucleotide into a vessel for delivery to the cell. 54

Inventors: BUDKER VLADIMIR G, KNECHTLE STUART J, WOLFF JON A, BUDKER, VLADIMIR, G., KNECHTLE, STUART, J., WOLFF, JON, A.
Applicants: MIRUS CORP, MIRUS CORPORATION
Application year: 1999
Main classification: A61K48
IPC codes: A61K35, A61K48, C12N15