BASB029 POLYNUCLEOTIDE(S) AND POLYPEPTIDES FROM NEISSERIA MENINGITIDIS

Abstract: The invention provides BASB029 polypeptides and polynucleotides encoding BASB029 polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are diagnostic, prophylactic and therapeutic uses.

Description: BASB029 POLYNUCLEOΗDE(S) AND POLYPEPTIDES FROM NEISSERIA MENINGITIDIS FIELD OF THE INVENTION This invention relates to polynucleotides, (herein referred to as "BASB029 polynucleotide(s)"), polypeptides encoded by them (referred to herein as "BASB029" or "BASB029 polypeptide(s)"), recombinant materials and methods for their production. In another aspect, the invention relates to methods for using such polypeptides and polynucleotides, including vaccines against bacterial infections. In a further aspect, the invention relates to diagnostic assays for detecting infection of certain pathogens. BACKGROUND OF THE INVENTION Neisseria meningitidis (meningococcus) is a Gram-negative bacterium frequently isolated from the human upper respiratory tract. It occasionally causes invasive bacterial diseases such as bacteremia and meningitis. The incidence of meningococcal disease shows geographical seasonal and annual differences (Schwartz, B., Moore, P.S., Broome, C.V.; Clin. Microbiol. Rev. 2 (Supplement), S18-S24, 1989). Most disease in temperate countries is due to strains of serogroup B and varies in incidence from 1-10/100,000/year total population sometimes reaching higher values (Kaczmarski, E.B. (1997), Commun. Dis. Rep. Rev. 7: R55-9, 1995; Scholten. R.J.P.M., Bijlmer, H.A., Poolman, J.T. et al. Clin. Infect. Dis. 16: 237-246, 1993; Cruz, C, Pavez. G., Aguilar, E., et al. Epidemiol. Infect. 105: 119-126, 1990). Epidemics dominated by serogroup A meningococci, mostly in central Africa, are encountered, sometimes reaching levels up to 1000/100.000/year (Schwartz, B., Moore, P.S., Broome, CN. Clin. Microbiol. Rev. 2 (Supplement), S18-S24, 1989). Nearly all cases as a whole of meningococcal disease are caused by serogroup A, B, C, W-135 and Y meningococci and a tetravalent A, C, W-135, Y polysaccharide vaccine is available (Armand, J., Arminjon, F., Mynard, M.C., Lafaix, C, J. Biol. Stand. 10: 335-339, 1982). The polysaccharide vaccines are currently being improved by way of chemical conjugating them to carrier proteins (Lieberman, J.M., Chiu, S.S., Wong, V.K., et al. JAMA 275 : 1499- 1503, 1996). A serogroup B vaccine is not available, since the B capsular polysaccharide was found to be nonimmunogenic, most likely because it shares structural similarity to host components (Wyle, F.A., Artenstein, M.S., Brandt, M.L. et al. J. Infect. Dis. 126: 514-522. 1972; Finne. J.M., Leinonen, M., Makela, P.M. Lancet ii.: 355-357, 1983). For many years efforts have been initiated and carried out to develop meningococcal outer membrane based vaccines (de Moraes, J.C.. Perkins, B., Camargo, M.C. et al. Lancet 340: 1074-1078, 1992; Bjune, G., Hoiby, E.A. Gronnesby, J.K. et al. 338: 1093-1096, 1991). Such vaccines have demonstrated efficacies from 57% - 85% in older children (>4 years) and adolescents. Many bacterial outer membrane components are present in these vaccines, such as PorA, PorB, Rmp. Ope, Opa, FrpB and the contribution of these components to the observed protection still needs futher definition. Other bacterial outer membrane components have been defined by using animal or human antibodies to be potentially relevant to the induction of protective immunity, such as TbpB and NspA (Martin. D., Cadieux. N.. Hamel, J.. Brodeux, B.R., J. Exp. Med. 185: 1173-1183, 1997; Lissolo, L.. Maitre-Wilmotte, C, Dumas, p. et al.. Inf. Immun. 63: 884-890, 1995). The mechanisms of protective immunity will involve antibody mediated bactericidal activity and opsonophagocytosis. A bacteremia animal model has been used to combine all antibody mediated mechanisms (Saukkonen, K., Leinonen, M., Abdillahi, H. Poolman, J. T. Vaccine 7: 325-328, 1989). It is generally accepted that the late complement component mediated bactericidal mechanism is crucial for immunity against meningococcal disease (Ross, S.C, Rosenthal P.J., Berberic. H.M., Densen, P. J. Infect. Dis. 155: 1266-1275, 1987). The frequency of Neisseria meningitidis infections has risen dramatically in the past few decades. This has been attributed to the emergence of multiply antibiotic resistant strains and an increasing population of people with weakened immune systems. It is no longer uncommon to isolate Neisseria meningitidis strains that are resistant to some or all ofthe standard antibiotics. This phenomenon has created an unmet medical need and demand for new anti-microbial agents, vaccines, drug screening methods, and diagnostic tests for this organism. SUMMARY OF THE INVENTION The present invention relates to BASB029, in particular BASB029 polypeptides and B ASB029 polynucleotides. recombinant materials and methods for their production. In another aspect, the invention relates to methods for using such polypeptides and polynucleotides, including prevention and treatment of microbial diseases, amongst others. In a further aspect, the invention relates to diagnostic assays for detecting diseases associated with microbial infections and conditions associated with such infections, such as assays for detecting expression or activity of BASB029 polynucleotides or polypeptides. Various changes and modifications within the spirit and scope ofthe disclosed invention will become readily apparent to those skilled in the art from reading the following descriptions and from reading the other parts ofthe present disclosure. DESCRIPTION OF THE INVENTION The invention relates to BASB029 polypeptides and polynucleotides as described in greater detail below. In particular, the invention relates to polypeptides and polynucleotides of BASB029 of Neisseria meningitidis, which is related by amino acid sequence homology to Haemophilus influenzae surface fibril (HSF) protein. The invention relates especially to BASB029 having the nucleotide and amino acid sequences set out in SEQ ID NO: 1,3 and SEQ ID NO:2,4 respectively. It is understood that sequences recited in the Sequence Listing below as "DNA" represent an exemplification of one embodiment ofthe invention, since those of ordinary skill will recognize that such sequences can be usefully employed in polynucleotides in general, including ribopolynucleotides. Polypeptides In one aspect ofthe invention there are provided polypeptides of Neisseria meningitidis referred to herein as "BASB029" and "BASB029 polypeptides" as well as biologically, diagnostically, prophylactically, clinically or therapeutically useful variants thereof, and compositions comprising the same. The present invention further provides for: (a) an isolated polypeptide which comprises an amino acid sequence which has at least 85% identity, more preferably at least 90% identity, yet more preferably at least 95% identity, most preferably at least 97-99% or exact identity, to that of SEQ ID NO:2. 4; (b) a polypeptide encoded by an isolated polynucleotide comprising a polynucleotide sequence which has at least 85% identity, more preferably at least 90% identity, yet more preferably at least 95% identity, even more preferably at least 97-99% or exact identity to SEQ ID NO: 1 , 3 over the entire length of SEQ ID NO: 1 , 3 respectively; or (c) a polypeptide encoded by an isolated polynucleotide comprising a polynucleotide sequence encoding a polypeptide which has at least 85% identity, more preferably at least 90% identity, yet more preferably at least 95% identity, even more preferably at least 97- 99% or exact identity, to the amino acid sequence of SEQ ID NO:2, 4; The BASB029 polypeptides provided in SEQ ID NO:2.4 are the BASB029 polypeptides from Neisseria meningitidis strains ATCC 13090 and H44/76. The invention also provides an immunogenic fragment of a BASB029 polypeptide. that is, a contiguous portion ofthe BASB029 polypeptide which has the same or substantially the same immunogenic activity as the polypeptide comprising the amino acid sequence of SEQ ID NO:2,4. That is to say, the fragment (if necessary when coupled to a carrier) is capable of raising an immune response which recognises the BASB029 polypeptide. Such an immunogenic fragment may include, for example, the BASB029 polypeptide lacking an N-terminal leader sequence, and/or a transmembrane domain and/or a C- terminal anchor domain. In a preferred aspect the immunogenic fragment of BASB029 according to the invention comprises substantially all ofthe extracellular domain of a polypeptide which has at least 85% identity, more preferably at least 90% identity, yet more preferably at least 95% identity, most preferably at least 97-99% identity, to that of SEQ ID NO:2,4 over the entire length of SEQ ID NO:2 A fragment is a polypeptide having an amino acid sequence that is entirely the same as part but not all of any amino acid sequence of any polypeptide of the invention. As with BASB029 polypeptides, fragments may be "free-standing," or comprised within a larger polypeptide of which they form a part or region, most preferably as a single continuous region in a single larger polypeptide. Preferred fragments include, for example, truncation polypeptides having a portion of an amino acid sequence of SEQ ID NO:2,4 or of variants thereof, such as a continuous series of residues that includes an amino- and or carboxy 1-terminal amino acid sequence. Degradation forms of the polypeptides of the invention produced by or in a host cell, are also preferred. Further preferred are fragments characterized by structural or functional attributes such as fragments that comprise alpha-helix and alpha-helix forming regions, beta-sheet and beta-sheet-forming regions, turn and turn-forming regions, coil and coil- forming regions, hydrophilic regions, hydrophobic regions, alpha amphipathic regions, beta amphipathic regions, flexible regions, surface-forming regions, substrate binding region, and high antigenic index regions. Further preferred fragments include an isolated polypeptide comprising an amino acid sequence having at least 15. 20. 30, 40, 50 or 100 contiguous amino acids from the amino acid sequence of SEQ ID NO:2,4, or an isolated polypeptide comprising an amino acid sequence having at least 15, 20, 30, 40, 50 or 100 contiguous amino acids truncated or deleted from the amino acid sequence of SEQ ID NO:2,4. Fragments ofthe polypeptides ofthe invention may be employed for producing the corresponding full-length polypeptide by peptide synthesis; therefore, these fragments may be employed as intermediates for producing the full-length polypeptides ofthe invention. Particularly preferred are variants in which several, 5-10, 1-5, 1-3, 1-2 or 1 amino acids are substituted, deleted, or added in any combination. The polypeptides. or immunogenic fragments, of the invention may be in the form of the "mature " protein or may be a part of a larger protein such as a precursor or a fusion protein. It is often advantageous to include an additional amino acid sequence which contains secretory or leader sequences, pro-sequences, sequences which aid in purification such as multiple histidine residues, or an additional sequence for stability during recombinant production. Furthermore, addition of exogenous polypeptide or lipid tail or polynucleotide sequences to increase the immunogenic potential ofthe final molecule is also considered. In one aspect, the invention relates to genetically engineered soluble fusion proteins comprising a polypeptide ofthe present invention, or a fragment thereof, and various portions ofthe constant regions of heavy or light chains of immunoglobulms of various subclasses (IgG, IgM, IgA, IgE). Preferred as an immunoglobulin is the constant part of the heavy chain of human IgG, particularly IgGl, where fusion takes place at the hinge region. In a particular embodiment, the Fc part can be removed simply by incorporation of a cleavage sequence which can be cleaved with blood clotting factor Xa. Furthermore, this invention relates to processes for the preparation of these fusion proteins by genetic engineering, and to the use thereof for drug screening, diagnosis and therapy. A further aspect ofthe invention also relates to polynucleotides encoding such fusion proteins. Examples of fusion protein technology can be found in International Patent Application Nos. WO94/29458 and WO94/22914. The proteins may be chemically conjugated, or expressed as recombinant fusion proteins allowing increased levels to be produced in an expression system as compared to non-fused protein. The fusion partner may assist in providing T helper epitopes (immunological fusion partner), preferably T helper epitopes recognised by humans, or assist in expressing the protein (expression enhancer) at higher yields than the native recombinant protein. Preferably the fusion partner will be both an immunological fusion partner and expression enhancing partner. Fusion partners include protein D from Haemophilus influenzas, and the non-structural protein from influenzae virus, NS1 (hemagglutinin). Another fusion partner is the protein known as LytA. Preferably the C terminal portion ofthe molecule is used. LytA is derived from Streptococcus pneumoniae which synthesize an N-acetyl-L- alanine amidase, amidase LytA, (coded by the lytA gene {Gene, 43 (1986) page 265- 272}) an autolysin that specifically degrades certain bonds in the peptidoglycan backbone. The C-terminal domain ofthe LytA protein is responsible for the affinity to the choline or to some choline analogues such as DEAE. This property has been exploited for the development of E. coli C-LytA expressing plasmids useful for expression of fusion proteins. Purification of hybrid proteins containing the C-LytA fragment at its amino terminus has been described {Biotechnology: 10, (1992) page 795-798}. It is possible to use the repeat portion ofthe LytA molecule found in the C terminal end starting at residue 178, for example residues 188 - 305. The present invention also includes variants ofthe aforementioned polypeptides, that is polypeptides that vary from the referents by conservative amino acid substitutions, whereby a residue is substituted by another with like characteristics. Typical such substitutions are among Ala, Val, Leu and He; among Ser and Thr; among the acidic residues Asp and Glu; among Asn and Gin; and among the basic residues Lys and Arg; or aromatic residues Phe and Tyr. Polypeptides ofthe present invention can be prepared in any suitable manner. Such polypeptides include isolated naturally occurring polypeptides, recombinantly produced polypeptides, synthetically produced polypeptides, or polypeptides produced by a combination of these methods. Means for preparing such polypeptides are well understood in the art. It is most preferred that a polypeptide ofthe invention is derived from Neisseria meningitidis. however, it may preferably be obtained from other organisms ofthe same taxonomic genus. A polypeptide ofthe invention may also be obtained, for example, from organisms ofthe same taxonomic family or order. Polynucleotides It is an object ofthe invention to provide polynucleotides that encode BASB029 polypeptides, particularly polynucleotides that encode the polypeptide herein designated BASB029. In a particularly preferred embodiment ofthe invention the polynucleotide comprises a region encoding BASB029 polypeptides comprising a sequence set out in SEQ ID NO: 1,3 which includes a full length gene, or a variant thereof. The BASB029 polynucleotides provided in SEQ ID NO: 1 ,3 are the BASB029 polynucleotides from Neisseria meningitidis strains ATCC 13090 and H44/76. As a further aspect ofthe invention there are provided isolated nucleic acid molecules encoding and/or expressing BASB029 polypeptides and polynucleotides, particularly Neisseria meningitidis B ASB029 polypeptides and polynucleotides, including, for example, unprocessed RNAs, ribozyme RNAs, mRNAs, cDNAs, genomic DNAs, B- and Z-DNAs. Further embodiments ofthe invention include biologically, diagnostically, prophylactically, clinically or therapeutically useful polynucleotides and polypeptides, and variants thereof, and compositions comprising the same. Another aspect ofthe invention relates to isolated polynucleotides, including at least one full length gene, that encodes a BASB029 polypeptide having a deduced amino acid sequence of SEQ ID NO:2,4 and polynucleotides closely related thereto and variants thereof. In another particularly preferred embodiment ofthe invention there is a BASB029 polypeptide from Neisseria meningitidis comprising or consisting of an amino acid sequence of SEQ ID NO:2,4 or a variant thereof. Using the information provided herein, such as a polynucleotide sequence set out in SEQ ID NO: 1. 3 a polynucleotide ofthe invention encoding BASB029 polypeptide may be obtained using standard cloning and screening methods, such as those for cloning and sequencing chromosomal DNA fragments from bacteria using Neisseria meningitidis cells as starting material, followed by obtaining a full length clone. For example, to obtain a polynucleotide sequence ofthe invention, such as a polynucleotide sequence given in SEQ ID NO: 1,3, typically a library of clones of chromosomal DNA of Neisseria meningitidis in E.coli or some other suitable host is probed with a radiolabeled oligonucleotide, preferably a 17- mer or longer, derived from a partial sequence. Clones carrying DNA identical to that of the probe can then be distinguished using stringent hybridization conditions. By sequencing the individual clones thus identified by hybridization with sequencing primers designed from the original polypeptide or polynucleotide sequence it is then possible to extend the polynucleotide sequence in both directions to determine a full length gene sequence. Conveniently, such sequencing is performed, for example, using denatured double stranded DNA prepared from a plasmid clone. Suitable techniques are described by Maniatis, T., Fritsch, E.F. and Sambrook et al., MOLECULAR CLONING, A LABORATORY MANUAL, 2nd Ed.; Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York (1989). (see in particular Screening By Hybridization 1.90 and Sequencing Denatured Double-Stranded DNA Templates 13.70). Direct genomic DNA sequencing may also be performed to obtain a full length gene sequence. Illustrative of the invention, each polynucleotide set out in SEQ ID NO: 1.3 was discovered in a DNA library derived from Neisseria meningitidis. Moreover, each DNA sequence set out in SEQ ID NO: 1,3 contains an open reading frame encoding a protein having about the number of amino acid residues set forth in SEQ ID NO:2, 4 with a deduced molecular weight that can be calculated using amino acid residue molecular weight values well known to those skilled in the art. The polynucleotide of SEQ ID NO:l, between the start codon at nucleotide number 1 and the stop codon which begins at nucleotide number 1783 of SEQ ID NO:l, encodes the polypeptide of SEQ ID NO:2. The polynucleotide of SEQ ID NO:3, between the start codon at nucleotide number 1 and the stop codon which begins at nucleotide number 1774 of SEQ ID NO:3, encodes the polypeptide of SEQ ID NO:4. In a further aspect, the present invention provides for an isolated polynucleotide comprising or consisting of: (a) a polynucleotide sequence which has at least 85% identity, more preferably at least 90% identity, yet more preferably at least 95%) identity, even more preferably at least 97-99% or exact identity to SEQ ID NO: 1 ,3 over the entire length of SEQ ID NO: 1 ,3 respectively; or (b) a polynucleotide sequence encoding a polypeptide which has at least 85% identity, more preferably at least 90% identity, yet more preferably at least 95% identity, even more preferably at least 97-99% or 100% exact, to the amino acid sequence of SEQ ID NO:2, 4 over the entire length of SEQ ID NO:2, 4 respectively. A polynucleotide encoding a polypeptide ofthe present invention, including homologs and orthologs from species other than Neisseria meningitidis, may be obtained by a process which comprises the steps of screening an appropriate library under stringent hybridization conditions (for example, using a temperature in the range of 45 - 65 °C and an SDS concentration from 0.1 - 1%) with a labeled or detectable probe consisting of or comprising the sequence of SEQ ID NO: 1, 3 or a fragment thereof; and isolating a full-length gene and/or genomic clones containing said polynucleotide sequence. The invention provides a polynucleotide sequence identical over its entire length to a coding sequence (open reading frame) in SEQ ID NO: 1, 3. Also provided by the invention is a coding sequence for a mature polypeptide or a fragment thereof, by itself as well as a coding sequence for a mature polypeptide or a fragment in reading frame with another coding sequence, such as a sequence encoding a leader or secretory sequence, a pre-, or pro- or prepro-protein sequence. The polynucleotide ofthe invention may also contain at least one non-coding sequence, including for example, but not limited to at least one non-coding 5' and 3 ' sequence, such as the transcribed but non-translated sequences, termination signals (such as rho-dependent and rho-independent termination signals), ribosome binding sites, Kozak sequences, sequences that stabilize mRNA, introns, and polyadenylation signals. The polynucleotide sequence may also comprise additional coding sequence encoding additional amino acids. For example, a marker sequence that facilitates purification ofthe fused polypeptide can be encoded. In certain embodiments ofthe invention, the marker sequence is a hexa-histidine peptide, as provided in the pQE vector (Qiagen. Inc.) and described in Gentz et al., Proc. Natl. Acad. Sci., USA 86: 821-824 (1989), or an HA peptide tag (Wilson et al, Cell 37: 767 (1984), both of which may be useful in purifying polypeptide sequence fused to them. Polynucleotides ofthe invention also include, but are not limited to, polynucleotides comprising a structural gene and its naturally associated sequences that control gene expression. The nucleotide sequence encoding BASB029 polypeptide of SEQ ID NO:2. 4 may be identical to the polypeptide encoding sequence contained in nudeotides 1 to 1782 of SEQ ID NO: l . or the polypeptide encoding sequence contained in nudeotides 1 to 1773 of SEQ ID NO:3, respectively. Alternatively it may be a sequence, which as a result ofthe redundancy (degeneracy) ofthe genetic code, also encodes the polypeptide of SEQ ID NO:2, 4. The term "polynucleotide encoding a polypeptide" as used herein encompasses polynucleotides that include a sequence encoding a polypeptide ofthe invention, particularly a bacterial polypeptide and more particularly a polypeptide ofthe Neisseria meningitidis BASB029 having an amino acid sequence set out in SEQ ID NO:2. 4. The term also encompasses polynucleotides that include a single continuous region or discontinuous regions encoding the polypeptide (for example, polynucleotides interrupted by integrated phage, an integrated insertion sequence, an integrated vector sequence, an integrated transposon sequence, or due to RNA editing or genomic DNA reorganization) together with additional regions, that also may contain coding and/or non-coding sequences. The invention further relates to variants ofthe polynucleotides described herein that encode variants of a polypeptide having a deduced amino acid sequence of SEQ ID NO:2. 4. Fragments of polynucleotides ofthe invention may be used, for example, to synthesize full- length polynucleotides ofthe invention. Further particularly preferred embodiments are polynucleotides encoding BASB029 variants, that have the amino acid sequence of BASB029 polypeptide of SEQ ID NO:2, 4 in which several, a few, 5 to 10, 1 to 5, 1 to 3, 2, 1 or no amino acid residues are substituted, modified, deleted and/or added, in any combination. Especially preferred among these are silent substitutions, additions and deletions, that do not alter the properties and activities of BASB029 polypeptide. Further preferred embodiments ofthe invention are polynucleotides that are at least 85% identical over their entire length to a polynucleotide encoding BASB029 polypeptide having an amino acid sequence set out in SEQ ID NO:2, 4, and polynucleotides that are complementary to such polynucleotides. In this regard, polynucleotides at least 90% identical over their entire length to the same are particularly preferred, and among these particularly preferred polynucleotides, those with at least 95% are especially preferred. Furthermore, those with at least 97% are highly preferred among those with at least 95%, and among these those with at least 98% and at least 99% are particularly highly preferred, with at least 99% being the more preferred. Preferred embodiments are polynucleotides encoding polypeptides that retain substantially the same biological function or activity as the mature polypeptide encoded by a DNA of SEQ ID NO: 1, 3. In accordance with certain preferred embodiments of this invention there are provided polynucleotides that hybridize, particularly under stringent conditions, to BASB029 polynucleotide sequences, such as those polynucleotides in SEQ ID NO:l, 3. The invention further relates to polynucleotides that hybridize to the polynucleotide sequences provided herein. In this regard, the invention especially relates to polynucleotides that hybridize under stringent conditions to the polynucleotides described herein. As herein used, the terms "stringent conditions" and "stringent hybridization conditions" mean hybridization occurring only if there is at least 95% and preferably at least 97% identity between the sequences. A specific example of stringent hybridization conditions is overnight incubation at 42°C in a solution comprising: 50% formamide, 5x SSC (150mM NaCl. 15mM trisodium citrate), 50 mM sodium phosphate (pH7.6), 5x Denhardt's solution, 10%) dextran sulfate, and 20 micrograms/ml of denatured, sheared salmon sperm DNA, followed by washing the hybridization support in 0. lx SSC at about 65°C. Hybridization and wash conditions are well known and exemplified in Sambrook. et al.. Molecular Cloning: A Laboratory Manual. Second Edition, Cold Spring Harbor. N.Y.. (1989), particularly Chapter 11 therein. Solution hybridization may also be used with the polynucleotide sequences provided by the invention. The invention also provides a polynucleotide consisting of or comprising a polynucleotide sequence obtained by screening an appropriate library containing the complete gene for a polynucleotide sequence set forth in SEQ ID NO:l, 3 under stringent hybridization conditions with a probe having the sequence of said polynucleotide sequence set forth in SEQ ID NO:l, 3 or a fragment thereof; and isolating said polynucleotide sequence. Fragments useful for obtaining such a polynucleotide include, for example, probes and primers fully described elsewhere herein. As discussed elsewhere herein regarding polynucleotide assays ofthe invention, for instance, the polynucleotides ofthe invention, may be used as a hybridization probe for RNA, cDNA and genomic DNA to isolate full-length cDNAs and genomic clones encoding BASB029 and to isolate cDNA and genomic clones of other genes that have a high identity, particularly high sequence identity, to the BASB029 gene. Such probes generally will comprise at least 15 nucleotide residues or base pairs. Preferably, such probes will have at least 30 nucleotide residues or base pairs and may have at least 50 nucleotide residues or base pairs. Particularly preferred probes will have at least 20 nucleotide residues or base pairs and will have less than 30 nucleotide residues or base pairs. A coding region of a BASB029 gene may be isolated by screening using a DNA sequence provided in SEQ ID NO: 1. 3 to synthesize an oligonucleotide probe. A labeled oligonucleotide having a sequence complementary to that of a gene ofthe invention is then used to screen a library of cDNA, genomic DNA or mRNA to determine which members of the library the probe hybridizes to. There are several methods available and well known to those skilled in the art to obtain full-length DNAs, or extend short DNAs, for example those based on the method of Rapid Amplification of cDNA ends (RACE) (see, for example. Frohman, et al, PNAS USA 85: 8998-9002, 1988). Recent modifications ofthe technique, exemplified by the Marathon™ technology (Clontech Laboratories Inc.) for example, have significantly simplified the search for longer cDNAs. In the Marathon™ technology, cDNAs have been prepared from mRNA extracted from a chosen tissue and an 'adaptor' sequence ligated onto each end. Nucleic acid amplification (PCR) is then carried out to amplify the "missing" 5' end ofthe DNA using a combination of gene specific and adaptor specific oligonucleotide primers. The PCR reaction is then repeated using "nested" primers, that is, primers designed to anneal within the amplified product (typically an adaptor specific primer that anneals further 3' in the adaptor sequence and a gene specific primer that anneals further 5' in the selected gene sequence). The products of this reaction can then be analyzed by DNA sequencing and a full-length DNA constructed either by joining the product directly to the existing DNA to give a complete sequence, or carrying out a separate full-length PCR using the new sequence information for the design of the 5' primer. The polynucleotides and polypeptides ofthe invention may be employed, for example, as research reagents and materials for discovery of treatments of and diagnostics for diseases, particularly human diseases, as further discussed herein relating to polynucleotide assays. The polynucleotides ofthe invention that are oligonucleotides derived from a sequence of SEQ ID NOS:l - 4 may be used in the processes herein as described, but preferably for PCR. to determine whether or not the polynucleotides identified herein in whole or in part are transcribed in bacteria in infected tissue. It is recognized that such sequences will also have utility in diagnosis ofthe stage of infection and type of infection the pathogen has attained. The invention also provides polynucleotides that encode a polypeptide that is the mature protein plus additional amino or carboxyl-terminal amino acids, or amino acids interior to the mature polypeptide (when the mature form has more than one polypeptide chain, for instance). Such sequences may play a role in processing of a protein from precursor to a mature form, may allow protein transport, may lengthen or shorten protein half-life or may facilitate manipulation of a protein for assay or production, among other things. As generally is the case in vivo, the additional amino acids may be processed away from the mature protein by cellular enzymes. For each and every polynucleotide ofthe invention there is provided a polynucleotide complementary to it. It is preferred that these complementary polynucleotides are fully complementary to each polynucleotide with which they are complementary. A precursor protein, having a mature form ofthe polypeptide fused to one or more prosequences may be an inactive form ofthe polypeptide. When prosequences are removed such inactive precursors generally are activated. Some or all ofthe prosequences may be removed before activation. Generally, such precursors are called proproteins. In addition to the standard A, G, C, T/U representations for nudeotides, the term "N" may also be used in describing certain polynucleotides ofthe invention. "N" means that any of the four DNA or RNA nudeotides may appear at such a designated position in the DNA or RNA sequence, except it is preferred that N is not a nucleic acid that when taken in combination with adjacent nucleotide positions, when read in the correct reading frame, would have the effect of generating a premature termination codon in such reading frame. In sum. a polynucleotide ofthe invention may encode a mature protein, a mature protein plus a leader sequence (which may be referred to as a preprotein), a precursor of a mature protein having one or more prosequences that are not the leader sequences of a preprotein. or a preproprotein, which is a precursor to a proprotein, having a leader sequence and one or more prosequences. which generally are removed during processing steps that produce active and mature forms ofthe polypeptide. In accordance with an aspect of the invention, there is provided the use of a polynucleotide ofthe invention for therapeutic or prophylactic purposes, in particular genetic immunization. The use of a polynucleotide of the invention in genetic immunization will preferably employ a suitable delivery method such as direct injection of plasmid DNA into muscles (Wolff et al, Hum Mol Genet (1992) 1 : 363. Manthorpe et al, Hum. Gene Ther. (1983) 4: 419), delivery of DNA complexed with specific protein carriers (Wu et al., J Biol Chem. (1989) 264: 16985), coprecipitation of DNA with calcium phosphate (Benvenisty & Reshef, PNAS USA, (1986) 83: 9551), encapsulation of DNA in various forms of liposomes (Kaneda et al, Science (1989) 243: 375), particle bombardment (Tang et al, Nature (1992) 356:152, Eisenbraun et al, DNA Cell Biol (1993) 12: 791) and in vivo infection using cloned retroviral vectors (Seeger et al, PNAS USA (1984) 81 : 5849). Vectors, Host Cells, Expression Systems The invention also relates to vectors that comprise a polynucleotide or polynucleotides of the invention, host cells that are genetically engineered with vectors o the invention and the production of polypeptides ofthe invention by recombinant techniques. Cell-free translation systems can also be employed to produce such proteins using RNAs derived from the DNA constructs ofthe invention. Recombinant polypeptides ofthe present invention may be prepared by processes well known in those skilled in the art from genetically engineered host cells comprising expression systems. Accordingly, in a further aspect, the present invention relates to expression systems that comprise a polynucleotide or polynucleotides ofthe present invention, to host cells which are genetically engineered with such expression systems, and to the production of polypeptides ofthe invention by recombinant techniques. For recombinant production ofthe polypeptides ofthe invention, host cells can be genetically engineered to incorporate expression systems or portions thereof or polynucleotides ofthe invention. Introduction of a polynucleotide into the host cell can be effected by methods described in many standard laboratory manuals, such as Davis, et al.. BASIC METHODS IN MOLECULAR BIOLOGY, (1986) and Sambrook, et al. MOLECULAR CLONING: A LABORATORY MANUAL. 2nd Ed.. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, N.Y. (1989). such as. calcium phosphate transfection. DEAE-dextran mediated transfection, transvection. microinjection, cationic lipid-mediated transfection. electroporation, transduction. scrape loading, ballistic introduction and infection. Representative examples of appropriate hosts include bacterial cells, such as cells of streptococci, staphylococci. enterococci, E. coli, streptomyces. cyanobacteria, Bacillus subtilis, Moraxella catarrhalis, Haemophilus influenzae and Neisseria meningitidis; fungal cells, such as cells of a yeast, Kluveromyces, Saccharomyces, a basidiomycete, Candida albicans and Aspergillus; insect cells such as cells of Drosophila S2 and Spodoptera Sf9; animal cells such as CHO, COS. HeLa. C127, 3T3, BHK, 293, CV-1 and Bowes melanoma cells; and plant cells, such as cells of a gymnosperm or angiosperm. A great variety of expression systems can be used to produce the polypeptides ofthe invention. Such vectors include, among others, chromosomal-, episomal- and virus-derived vectors, for example, vectors derived from bacterial plasmids, from bacteriophage, from transposons, from yeast episomes, from insertion elements, from yeast chromosomal elements, from viruses such as baculoviruses, papova viruses, such as SV40. vaccinia viruses, adenoviruses, fowl pox viruses, pseudorabies viruses, picomaviruses. retroviruses, and alphaviruses and vectors derived from combinations thereof, such as those derived from plasmid and bacteriophage genetic elements, such as cosmids and phagemids. The expression system constructs may contain control regions that regulate as well as engender expression. Generally, any system or vector suitable to maintain, propagate or express polynucleotides and/or to express a polypeptide in a host may be used for expression in this regard. The appropriate DNA sequence may be inserted into the expression system by any of a variety of well-known and routine techniques, such as, for example, those set forth in Sambrook et al, MOLECULAR CLONING, A LABORATORY MANUAL, (supra). In recombinant expression systems in eukaryotes, for secretion of a translated protein into the lumen ofthe endoplasmic reticulum. into the periplasmic space or into the extracellular environment, appropriate secretion signals may be incoφorated into the expressed polypeptide. These signals may be endogenous to the polypeptide or they may be heterologous signals. Polypeptides ofthe present invention can be recovered and purified from recombinant cell cultures by well-known methods including ammonium sulfate or ethanol precipitation, acid extraction, anion or cation exchange chromatography, phosphocellulose chromatography, hydrophobic interaction chromatography, affinity chromatography, hydroxy lapatite chromatography and lectin chromatography. Most preferably, ion metal affinity chromatography (IMAC) is employed for purification. Well known techniques for refolding proteins may be employed to regenerate active conformation when the polypeptide is denatured during intracellular synthesis, isolation and or purification. The expression system may also be a recombinant live microorganism, such as a virus or bacterium. The gene of interest can be inserted into the genome of a live recombinant virus or bacterium. Inoculation and in vivo infection with this live vector will lead to in vivo expression ofthe antigen and induction of immune responses. Viruses and bacteria used for this puφose are for instance: poxviruses (e.g; vaccinia, fowlpox, canarypox). alphaviruses (Sindbis virus. Semliki Forest Virus, Venezuelian Equine Encephalitis Virus), adenoviruses, adeno-associated virus, picomaviruses (poliovirus, rhinovirus). heφesviruses (varicella zoster virus, etc), Listeria, Salmonella . Shigella, Neisseria, BCG. These viruses and bacteria can be virulent, or attenuated in various ways in order to obtain live vaccines. Such live vaccines also form part of the invention. Diagnostic, Prognostic, Serotyping and Mutation Assays This invention is also related to the use of BASB029 polynucleotides and polypeptides of the invention for use as diagnostic reagents. Detection of BASB029 polynucleotides and/or polypeptides in a eukaryote, particularly a mammal, and especially a human, will provide a diagnostic method for diagnosis of disease, staging of disease or response of an infectious organism to drugs. Eukaryotes, particularly mammals, and especially humans, particularly those infected or suspected to be infected with an organism comprising the BASB029 gene or protein, may be detected at the nucleic acid or amino acid level by a variety of well known techniques as well as by methods provided herein. Polypeptides and polynucleotides for prognosis, diagnosis or other analysis may be obtained from a putatively infected and/or infected individual's bodily materials. Polynucleotides from any of these sources, particularly DNA or RNA, may be used directly for detection or may be amplified enzymatically by using PCR or any other amplification technique prior to analysis. RNA, particularly mRNA, cDNA and genomic DNA may also be used in the same ways. Using amplification, characterization ofthe species and strain of infectious or resident organism present in an individual, may be made by an analysis ofthe genotype of a selected polynucleotide ofthe organism. Deletions and insertions can be detected by a change in size ofthe amplified product in comparison to a genotype of a reference sequence selected from a related organism, preferably a different species ofthe same genus or a different strain ofthe same species. Point mutations can be identified by hybridizing amplified DNA to labeled BASB029 polynucleotide sequences. Perfectly or significantly matched sequences can be distinguished from imperfectly or more significantly mismatched duplexes by DNase or RNase digestion, for DNA or RNA respectively, or by detecting differences in melting temperatures or renaturation kinetics. Polynucleotide sequence differences may also be detected by alterations in the electrophoretic mobility of polynucleotide fragments in gels as compared to a reference sequence. This may be carried out with or without denaturing agents. Polynucleotide differences may also be detected by direct DNA or RNA sequencing. See, for example, Myers et al, Science, 230: 1242 (1985). Sequence changes at specific locations also may be revealed by nuclease protection assays, such as RNase, VI and SI protection assay or a chemical cleavage method. See, for example. Cotton et al, Proc. Natl. Acad. Sci., USA, 85: 4397-4401 (1985). In another embodiment, an array of oligonucleotides probes comprising BASB029 nucleotide sequence or fragments thereof can be constructed to conduct efficient screening of. for example, genetic mutations, serotype, taxonomic classification or identification. Array technology methods are well known and have general applicability and can be used to address a variety of questions in molecular genetics including gene expression, genetic linkage, and genetic variability (see, for example, Chee et al, Science. 274: 610 (1996)). Thus in another aspect, the present invention relates to a diagnostic kit which comprises: (a) a polynucleotide ofthe present invention, preferably the nucleotide sequence of SEQ ID NO: 1 , 3, or a fragment thereof ; (b) a nucleotide sequence complementary to that of (a); (c) a polypeptide ofthe present invention, preferably the polypeptide of SEQ ID NO:2, 4 or a fragment thereof; or (d) an antibody to a polypeptide ofthe present invention, preferably to the polypeptide of SEQ ID NO:2, 4. It will be appreciated that in any such kit, (a), (b), (c) or (d) may comprise a substantial component. Such a kit will be of use in diagnosing a disease or susceptibility to a disease, among others. This invention also relates to the use of polynucleotides ofthe present invention as diagnostic reagents. Detection of a mutated form of a polynucleotide ofthe invention, preferable, SEQ ID NO: 1, 3, which is associated with a disease or pathogenicity will provide a diagnostic tool that can add to, or define, a diagnosis of a disease, a prognosis of a course of disease, a determination of a stage of disease, or a susceptibility to a disease, which results from under-expression, over-expression or altered expression ofthe polynucleotide. Organisms, particularly infectious organisms, carrying mutations in such polynucleotide may be detected at the polynucleotide level by a variety of techniques, such as those described elsewhere herein. Cells from an organism carrying mutations or polymoφhisms (allelic variations) in a polynucleotide and/or polypeptide ofthe invention may also be detected at the polynucleotide or polypeptide level by a variety of techniques, to allow for serotyping, for example. For example, RT-PCR can be used to detect mutations in the RNA. It is particularly preferred to use RT-PCR in conjunction with automated detection systems, such as. for example. GeneScan. RNA. cDNA or genomic DNA may also be used for the same puφose. PCR. As an example, PCR primers complementary to a polynucleotide encoding BASB029 polypeptide can be used to identify and analyze mutations. The invention further provides primers with 1, 2, 3 or 4 nudeotides removed from the 5' and/or the 3' end. These primers may be used for, among other things, amplifying BASB029 DNA and/or RNA isolated from a sample derived from an individual, such as a bodily material. The primers may be used to amplify a polynucleotide isolated from an infected individual, such that the polynucleotide may then be subject to various techniques for elucidation ofthe polynucleotide sequence. In this way, mutations in the polynucleotide sequence may be detected and used to diagnose and/or prognose the infection or its stage or course, or to serotype and/or classify the infectious agent. The invention further provides a process for diagnosing disease, preferably bacterial infections, more preferably infections caused by Neisseria meningitidis, comprising determining from a sample derived from an individual, such as a bodily material, an increased level of expression of polynucleotide having a sequence of SEQ ID NO:l, 3. Increased or decreased expression of a B ASB029 polynucleotide can be measured using any on ofthe methods well known in the art for the quantitation of polynucleotides, such as, for example, amplification, PCR, RT-PCR, RNase protection, Northern blotting, spectrometry and other hybridization methods. In addition, a diagnostic assay in accordance with the invention for detecting over- expression of BASB029 polypeptide compared to normal control tissue samples may be used to detect the presence of an infection, for example. Assay techniques that can be used to determine levels of a BASB029 polypeptide, in a sample derived from a host, such as a bodily material, are well-known to those of skill in the art. Such assay methods include radioimmunoassays, competitive-binding assays, Western Blot analysis, antibody sandwich assays, antibody detection and ELISA assays. The polynucleotides ofthe invention may be used as components of polynucleotide aπays, preferably high density arrays or grids. These high density aπays are particularly useful for diagnostic and prognostic puφoses. For example, a set of spots each comprising a different gene, and further comprising a polynucleotide or 5 polynucleotides of the invention, may be used for probing, such as using hybridization or nucleic acid amplification, using a probe obtained or derived from a bodily sample, to determine the presence of a particular polynucleotide sequence or related sequence in an individual. Such a presence may indicate the presence of a pathogen, particularly Neisseria meningitidis, and may be useful in diagnosing and/or prognosing disease or a 10 course of disease. A grid comprising a number of variants ofthe polynucleotide sequence of SEQ ID NO:l. 3 are preferred. Also preferred is a grid comprising a number of variants of a polynucleotide sequence encoding the polypeptide sequence of SEQ ID NO:2, 4. 15 Antibodies The polypeptides and polynucleotides ofthe invention or variants thereof, or cells expressing the same can be used as immunogens to produce antibodies immunospecific for such polypeptides or polynucleotides respectively. 20 In certain preferred embodiments ofthe invention there are provided antibodies against BASB029 polypeptides or polynucleotides. Antibodies generated against the polypeptides or polynucleotides ofthe invention can be obtained by administering the polypeptides and/or polynucleotides ofthe invention, or O epitope-bearing fragments of either or both, analogues of either or both, or cells expressing either or both, to an animal, preferably a nonhuman, using routine protocols. For preparation of monoclonal antibodies, any technique known in the art that provides antibodies produced by continuous cell line cultures can be used. Examples include various techniques, such as those in Kohler, G. and Milstein, C, Nature 256: 495-497 (1975); Kozbor et al, Immunology Today 4: 72 (1983); Cole et al, pg. 77-96 in MONOCLONAL ANTIBODIES AND CANCER THERAPY, Alan R. Liss. Inc. (1985). Techniques for the production of single chain antibodies (U.S. Patent No. 4,946,778) can be adapted to produce single chain antibodies to polypeptides or polynucleotides of this invention. Also, transgenic mice, or other organisms or animals, such as other mammals, may be used to express humanized antibodies immunospecific to the polypeptides or polynucleotides ofthe invention. Alternatively, phage display technology may be utilized to select antibody genes with binding activities towards a polypeptide of the invention either from repertoires of PCR amplified v-genes of lymphocytes from humans screened for possessing anti-BASB029 or from naive libraries (McCafferty, et al, (1990), Nature 348, 552-554; Marks, et al, (1992) Biotechnology 10. 779-783). The affinity of these antibodies can also be improved by, for example, chain shuffling (Clackson et al, (1991) Nature 352: 628). The above-described antibodies may be employed to isolate or to identify clones expressing the polypeptides or polynucleotides ofthe invention to purify the polypeptides or polynucleotides by. for example, affinity chromatography. Thus, among others, antibodies against BASB029-polypeptide or BASB029-polynucleotide may be employed to treat infections, particularly bacterial infections. Polypeptide variants include antigenically, epitopically or immunologically equivalent variants form a particular aspect of this invention. Preferably, the antibody or variant thereof is modified to make it less immunogenic in the individual. For example, if the individual is human the antibody may most preferably be "humanized," where the complimentarity determining region or regions ofthe hybridoma- derived antibody has been transplanted into a human monoclonal antibody, for example as described in Jones et al. (1986), Nature 321, 522-525 or Tempest et al, (1991) Biotechnology 9, 266-273. Antagonists and Agonists - Assays and Molecules Polypeptides and polynucleotides ofthe invention may also be used to assess the binding of small molecule substrates and ligands in, for example, cells, cell-free preparations, chemical libraries, and natural product mixtures. These substrates and ligands may be natural substrates and ligands or may be structural or functional mimetics. See. e.g. , Coligan et al.. Current Protocols in Immunology 1(2): Chapter 5 (1991). The screening methods may simply measure the binding of a candidate compound to the polypeptide or polynucleotide, or to cells or membranes bearing the polypeptide or polynucleotide, or a fusion protein ofthe polypeptide by means of a label directly or indirectly associated with the candidate compound. Alternatively, the screening method may involve competition with a labeled competitor. Further, these screening methods may test whether the candidate compound results in a signal generated by activation or inhibition of the polypeptide or polynucleotide, using detection systems appropriate to the cells comprising the polypeptide or polynucleotide. Inhibitors of activation are generally assayed in the presence of a known agonist and the effect on activation by the agonist by the presence of the candidate compound is observed. Constitutively active polypeptide and/or constitutively expressed polypeptides and polynucleotides may be employed in screening methods for inverse agonists or inhibitors, in the absence of an agonist or inhibitor, by testing whether the candidate compound results in inhibition of activation of the polypeptide or polynucleotide, as the case may be. Further, the screening methods may simply comprise the steps of mixing a candidate compound with a solution containing a polypeptide or polynucleotide ofthe present invention, to form a mixture, measuring BASB029 polypeptide and/or polynucleotide activity in the mixture, and comparing the BASB029 polypeptide and/or polynucleotide activity ofthe mixture to a standard. Fusion proteins, such as those made from Fc portion and BASB029 polypeptide, as hereinbefore described, can also be used for high-throughput screening assays to identify antagonists ofthe polypeptide ofthe present invention, as well as of phylogenetically and and/or functionally related polypeptides (see D. Bennett et al.. J Mol Recognition. 8:52-58 (1995); and K. Johanson et al., J Biol Chem, 270(16):9459-9471 (1995)). The polynucleotides, polypeptides and antibodies that bind to and/or interact with a polypeptide ofthe present invention may also be used to configure screening methods for detecting the effect of added compounds on the production of mRNA and/or polypeptide in cells. For example, an ELISA assay may be constructed for measuring secreted or cell associated levels of polypeptide using monoclonal and polyclonal antibodies by standard methods known in the art. This can be used to discover agents which may inhibit or enhance the production of polypeptide (also called antagonist or agonist, respectively) from suitably manipulated cells or tissues. The invention also provides a method of screening compounds to identify those which enhance (agonist) or block (antagonist) the action of BASB029 polypeptides or polynucleotides, particularly those compounds that are bacteristatic and/or bactericidal. The method of screening may involve high-throughput techniques. For example, to screen for agonists or antagonists, a synthetic reaction mix, a cellular compartment, such as a membrane, cell envelope or cell wall, or a preparation of any thereof, comprising BASB029 polypeptide and a labeled substrate or ligand of such polypeptide is incubated in the absence or the presence of a candidate molecule that may be a BASB029 agonist or antagonist. The ability ofthe candidate molecule to agonize or antagonize the BASB029 polypeptide is reflected in decreased binding ofthe labeled ligand or decreased production of product from such substrate. Molecules that bind gratuitously, i.e., without inducing the effects of BASB029 polypeptide are most likely to be good antagonists. Molecules that bind well and, as the case may be. increase the rate of product production from substrate, increase signal transduction, or increase chemical channel activity are agonists. Detection ofthe rate or level of, as the case may be, production of product from substrate, signal transduction, or chemical channel activity may be enhanced by using a reporter system. Reporter systems that may be useful in this regard include but are not limited to colorimetric, labeled substrate converted into product, a reporter gene that is responsive to changes in BASB029 polynucleotide or polypeptide activity, and binding assays known in the art. Another example of an assay for BASB029 agonists is a competitive assay that combines BASB029 and a potential agonist with BASB029-binding molecules, recombinant BASB029 binding molecules, natural substrates or ligands, or substrate or ligand mimetics. under appropriate conditions for a competitive inhibition assay. BASB029 can be labeled. such as by radioactivity or a colorimetric compound, such that the number of BASB029 molecules bound to a binding molecule or converted to product can be determined accurately to assess the effectiveness ofthe potential antagonist. Potential antagonists include, among others, small organic molecules, peptides, polypeptides and antibodies that bind to a polynucleotide and/or polypeptide ofthe invention and thereby inhibit or extinguish its activity or expression. Potential antagonists also may be small organic molecules, a peptide, a polypeptide such as a closely related protein or antibody that binds the same sites on a binding molecule, such as a binding molecule, without inducing BASB029-induced activities, thereby preventing the action or expression of BASB029 polypeptides and/or polynucleotides by excluding BASB029 polypeptides and/or polynucleotides from binding. Potential antagonists include a small molecule that binds to and occupies the binding site of the polypeptide thereby preventing binding to cellular binding molecules, such that normal biological activity is prevented. Examples of small molecules include but are not limited to small organic molecules, peptides or peptide-like molecules. Other potential antagonists include antisense molecules (see Okano, J. Neurochem. 56: 560 (1991); OLIGODEOXYNUCLEOTIDES AS ANTISENSE INHIBITORS OF GENE EXPRESSION, CRC Press, Boca Raton, FL (1988), for a description of these molecules). Preferred potential antagonists include compounds related to and variants of BASB029. In a further aspect, the present invention relates to genetically engineered soluble fusion proteins comprising a polypeptide ofthe present invention, or a fragment thereof, and various portions ofthe constant regions of heavy or light chains of immunoglobulms of various subclasses (IgG, IgM, IgA, IgE). Preferred as an immunoglobulin is the constant part ofthe heavy chain of human IgG, particularly IgGl, where fusion takes place at the hinge region. In a particular embodiment, the Fc part can be removed simply by incoφoration of a cleavage sequence which can be cleaved with blood clotting factor Xa. Furthermore, this invention relates to processes for the preparation of these fusion proteins by genetic engineering, and to the use thereof for drug screening, diagnosis and therapy. A further aspect ofthe invention also relates to polynucleotides encoding such fusion proteins. Examples of fusion protein technology can be found in International Patent Application Nos. WO94/29458 and WO94/22914. Each of the polynucleotide sequences provided herein may be used in the discovery and development of antibacterial compounds. The encoded protein, upon expression, can be used as a target for the screening of antibacterial drugs. Additionally, the polynucleotide sequences encoding the amino terminal regions ofthe encoded protein or Shine-Delgarno or other translation facilitating sequences ofthe respective mRNA can be used to construct antisense sequences to control the expression ofthe coding sequence of interest. The invention also provides the use ofthe polypeptide, polynucleotide. agonist or antagonist ofthe invention to interfere with the initial physical interaction between a pathogen or pathogens and a eukaryotic, preferably mammalian, host responsible for sequelae of infection. In particular, the molecules ofthe invention may be used: in the prevention of adhesion of bacteria, in particular gram positive and/or gram negative bacteria, to eukaryotic, preferably mammalian, extracellular matrix proteins on indwelling devices or to extracellular matrix proteins in wounds; to block bacterial adhesion between eukaryotic, preferably mammalian, extracellular matrix proteins and bacterial BASB029 proteins that mediate tissue damage and/or; to block the normal progression of pathogenesis in infections initiated other than by the implantation of in-dwelling devices or by other surgical techniques. In accordance with yet another aspect ofthe invention, there are provided BASB029 agonists and antagonists, preferably bacteristatic or bactericidal agonists and antagonists. The antagonists and agonists ofthe invention may be employed, for instance, to prevent, inhibit and/or treat diseases. In a further aspect, the present invention relates to mimotopes ofthe polypeptide ofthe invention. A mimotope is a peptide sequence, sufficiently similar to the native peptide (sequentially or structurally), which is capable of being recognised by antibodies which recognise the native peptide; or is capable of raising antibodies which recognise the native peptide when coupled to a suitable carrier. Peptide mimotopes may be designed for a particular puφose by addition, deletion or substitution of elected amino acids. Thus, the peptides may be modified for the puφoses of ease of conjugation to a protein carrier. For example, it may be desirable for some chemical conjugation methods to include a terminal cysteine. In addition it may be desirable for peptides conjugated to a protein earner to include a hydrophobic terminus distal from the conjugated terminus ofthe peptide, such that the free unconjugated end of the peptide remains associated with the surface of the carrier protein. Thereby presenting the peptide in a conformation which most closely resembles that ofthe peptide as found in the context ofthe whole native molecule. For example, the peptides may be altered to have an N-terminal cysteine and a C-terminal hydrophobic amidated tail. Alternatively, the addition or substitution of a D-stereoisomer form of one or more ofthe amino acids may be performed to create a beneficial derivative, for example to enhance stability ofthe peptide. Alternatively, peptide mimotopes may be identified using antibodies which are capable themselves of binding to the polypeptides ofthe present invention using techniques such as phage display technology (EP 0 552 267 BI). This technique, generates a large number of peptide sequences which mimic the structure ofthe native peptides and are. therefore, capable of binding to anti-native peptide antibodies, but may not necessarily themselves share significant sequence homology to the native polypeptide. Vaccines Another aspect ofthe invention relates to a method for inducing an immunological response in an individual, particularly a mammal, preferably humans, which comprises inoculating the individual with BASB029 polynucleotide and/or polypeptide, or a fragment or variant thereof, adequate to produce antibody and or T cell immune response to protect said individual from infection, particularly bacterial infection and most particularly Neisseria meningitidis infection. Also provided are methods whereby such immunological response slows bacterial replication. Yet another aspect ofthe invention relates to a method of inducing immunological response in an individual which comprises delivering to such individual a nucleic acid vector, sequence or ribozyme to direct expression of B ASB029 polynucleotide and/or polypeptide, or a fragment or a variant thereof, for expressing BASB029 polynucleotide and/or polypeptide, or a fragment or a variant thereof in vivo in order to induce an immunological response, such as, to produce antibody and/ or T cell immune response, including, for example, cytokine-producing T cells or cytotoxic T cells, to protect said individual, preferably a human, from disease, whether that disease is already established within the individual or not. One example of administering the gene is by accelerating it into the desired cells as a coating on particles or otherwise. Such nucleic acid vector may comprise DNA, RNA, a ribozyme, a modified nucleic acid, a DNA/RNA hybrid, a DNA-protein complex or an RNA-protein complex. A further aspect ofthe invention relates to an immunological composition that when introduced into an individual, preferably a human, capable of having induced within it an immunological response, induces an immunological response in such individual to a BASB029 polynucleotide and/or polypeptide encoded therefrom, wherein the composition comprises a recombinant BASB029 polynucleotide and/or polypeptide encoded therefrom and/or comprises DNA and/or RNA which encodes and expresses an antigen of said BASB029 polynucleotide, polypeptide encoded therefrom, or other polypeptide ofthe invention. The immunological response may be used therapeutically or prophylactically and may take the form of antibody immunity and/or cellular immunity, such as cellular immunity arising from CTL or CD4+ T cells. A B ASB029 polypeptide or a fragment thereof may be fused with co-protein or chemical moiety which may or may not by itself produce antibodies, but which is capable of stabilizing the first protein and producing a fused or modified protein which will have antigenic and/or immunogenic properties, and preferably protective properties. Thus fused recombinant protein, preferably further comprises an antigenic co-protein, such as lipoprotein D from Haemophilus influenzae, Glutathione-S-transferase (GST) or beta- galactosidase, or any other relatively large co-protein which solubilizes the protein and facilitates production and purification thereof. Moreover, the co-protein may act as an adjuvant in the sense of providing a generalized stimulation ofthe immune system of the organism receiving the protein. The co-protein may be attached to either the amino- or carboxy-terminus ofthe first protein. Provided by this invention are compositions, particularly vaccine compositions, and methods comprising the polypeptides and/or polynucleotides ofthe invention and immunostimulatory DNA sequences, such as those described in Sato, Y. et al. Science 273: 352 (1996). Also, provided by this invention are methods using the described polynucleotide or particular fragments thereof, which have been shown to encode non-variable regions of bacterial cell surface proteins, in polynucleotide constructs used in such genetic immunization experiments in animal models of infection with Neisseria meningitidis. Such experiments will be particularly useful for identifying protein epitopes able to provoke a prophylactic or therapeutic immune response. It is believed that this approach will allow for the subsequent preparation of monoclonal antibodies of particular value, derived from the requisite organ of the animal successfully resisting or clearing infection, for the development of prophylactic agents or therapeutic treatments of bacterial infection, particularly Neisseria meningitidis infection, in mammals, particularly humans. The invention also includes a vaccine formulation which comprises an immunogenic recombinant polypeptide and/or polynucleotide ofthe invention together with a suitable carrier, such as a pharmaceutically acceptable carrier. Since the polypeptides and polynucleotides may be broken down in the stomach, each is preferably administered parenterally, including, for example, administration that is subcutaneous, intramuscular, intravenous, or intradermal. Formulations suitable for parenteral administration include aqueous and non-aqueous sterile injection solutions which may contain anti-oxidants. buffers, bacteristatic compounds and solutes which render the formulation isotonic with the bodily fluid, preferably the blood, ofthe individual; and aqueous and non-aqueous sterile suspensions which may include suspending agents or thickening agents. The formulations may be presented in unit-dose or multi-dose containers, for example, sealed ampoules and vials and may be stored in a freeze-dried condition requiring only the addition ofthe sterile liquid carrier immediately prior to use. The vaccine formulation ofthe invention may also include adjuvant systems for enhancing the immunogenicity ofthe formulation. Preferably the adjuvant system raises preferentially a TH1 type of response. An immune response may be broadly distinguished into two extreme catagories. being a humoral or cell mediated immune responses (traditionally characterised by antibody and cellular effector mechanisms of protection respectively). These categories of response have been termed THl-type responses (cell-mediated response), and TH2-type immune responses (humoral response). Extreme THl-type immune responses may be characterised by the generation of antigen specific, haplotype restricted cytotoxic T lymphocytes, and natural killer cell responses. In mice THl-type responses are often characterised by the generation of antibodies of the IgG2a subtype, whilst in the human these coπespond to IgGl type antibodies. TH2- type immune responses are characterised by the generation of a broad range of immunoglobulin isotypes including in mice IgGl, IgA, and IgM. It can be considered that the driving force behind the development of these two types of immune responses are cytokines. High levels of THl-type cytokines tend to favour the induction of cell mediated immune responses to the given antigen, whilst high levels of TH2-type cytokines tend to favour the induction of humoral immune responses to the antigen. The distinction of TH1 and TH2-type immune responses is not absolute. In reality an individual will support an immune response which is described as being predominantly TH1 or predominantly TH2. However, it is often convenient to consider the families of cytokines in terms of that described in murine CD4 +ve T cell clones by Mosmann and Coffman (Mosmann, T.R. and Coffman, R.L. (1989) TH1 and TH2 cells: different patterns oflymphokine secretion lead to different functional properties. Annual Review of Immunology, 7, pi 45-173). Traditionally, THl-type responses are associated with the production ofthe INF-γ and IL-2 cytokines by T-lymphocytes. Other cytokines often directly associated with the induction of THl-type immune responses are not produced by T-cells, such as IL-12. In contrast, TH2- type responses are associated with the secretion of IL-4, IL-5. IL-6 and IL-13. It is known that certain vaccine adjuvants are particularly suited to the stimulation of either THl or TH2 - type cytokine responses. Traditionally the best indicators ofthe THl :TH2 balance ofthe immune response after a vaccination or infection includes direct measurement ofthe production of THl or TH2 cytokines by T lymphocytes in vitro after restimulation with antigen, and/or the measurement ofthe IgGl :IgG2a ratio of antigen specific antibody responses. Thus, a THl-type adjuvant is one which preferentially stimulates isolated T-cell populations to produce high levels of THl-type cytokines when re-stimulated with antigen in vitro, and promotes development of both CD8+ cytotoxic T lymphocytes and antigen specific immunoglobulin responses associated with THl-type isotype. Adjuvants which are capable of preferential stimulation ofthe THl cell response are described in International Patent Application No. WO 94/00153 and WO 95/17209. 3 De-O-acylated monophosphoryl lipid A (3D-MPL) is one such adjuvant. This is known from GB 222021 1 (Ribi). Chemically it is a mixture of 3 De-O-acylated monophosphoryl lipid A with 4, 5 or 6 acylated chains and is manufactured by Ribi Immunochem, Montana. A preferred form of 3 De-O-acylated monophosphoryl lipid A is disclosed in European Patent 0 689 454 BI (SmithKline Beecham Biologicals SA). Preferably, the particles of 3D-MPL are small enough to be sterile filtered through a 0.22micron membrane (European Patent number 0 689 454). 3D-MPL will be present in the range of lOμg - lOOμg preferably 25-50μg per dose wherein the antigen will typically be present in a range 2-50μg per dose. Another preferred adjuvant comprises QS21, an Hplc purified non-toxic fraction derived from the bark of Quillaja Saponaria Molina. Optionally this may be admixed with 3 De-O-acylated monophosphoryl lipid A (3D-MPL), optionally together with a carrier. The method of production of QS21 is disclosed in US patent No. 5,057.540. Non-reactogenic adjuvant formulations containing QS21 have been described previously (WO 96/33739). Such formulations comprising QS21 and cholesterol have been shown to be successful THl stimulating adjuvants when formulated together with an antigen. Further adjuvants which are preferential stimulators of THl cell response include immunomodulatory oligonucleotides, for example unmethylated CpG sequences as disclosed in WO 96/02555. Combinations of different THl stimulating adjuvants, such as those mentioned hereinabove. are also contemplated as providing an adjuvant which is a preferential stimulator of THl cell response. For example, QS21 can be formulated together with 3D-MPL. The ratio of QS21 : 3D-MPL will typically be in the order of 1 : 10 to 10 : 1; preferably 1 :5 to 5 : 1 and often substantially 1 : 1. The preferred range for optimal synergy is 2.5 : 1 to 1 : 1 3D-MPL: QS21. Preferably a carrier is also present in the vaccine composition according to the invention. The caπier may be an oil in water emulsion, or an aluminium salt, such as aluminium phosphate or aluminium hydroxide. A prefeπed oil-in-water emulsion comprises a metabolisible oil, such as squalene, alpha tocopherol and Tween 80. In a particularly preferred aspect the antigens in the vaccine composition according to the invention are combined with QS21 and 3D-MPL in such an emulsion. Additionally the oil in water emulsion may contain span 85 and/or lecithin and/or tricaprylin. Typically for human administration QS21 and 3D-MPL will be present in a vaccine in the range of l μg - 200μg, such as 10-100μg, preferably lOμg - 50μg per dose. Typically the oil in water will comprise from 2 to 10% squalene, from 2 to 10% alpha tocopherol and from 0.3 to 3% tween 80. Preferably the ratio of squalene: alpha tocopherol is equal to or less than 1 as this provides a more stable emulsion. Span 85 may also be present at a level of 1%. In some cases it may be advantageous that the vaccines ofthe present invention will further contain a stabiliser. Non-toxic oil in water emulsions preferably contain a non-toxic oil, e.g. squalane or squalene, an emulsifier, e.g. Tween 80, in an aqueous carrier. The aqueous caπier may be, for example, phosphate buffered saline. A particularly potent adjuvant formulation involving QS21, 3D-MPL and tocopherol in an oil in water emulsion is described in WO 95/17210. The present invention also provides a polyvalent vaccine composition comprising a vaccine formulation ofthe invention in combination with other antigens, in particular antigens useful for treating cancers, autoimmune diseases and related conditions. Such a polyvalent vaccine composition may include a TH-1 inducing adjuvant as hereinbefore described. While the invention has been described with reference to certain BASB029 polypeptides and polynucleotides, it is to be understood that this covers fragments ofthe naturally occurring polypeptides and polynucleotides. and similar polypeptides and polynucleotides with additions, deletions or substitutions which do not substantially affect the immunogenic properties ofthe recombinant polypeptides or polynucleotides. The antigen can also be delivered in the form of whole bacteria (dead or alive) or as subcellular fractions, these possibilities do include N. meningitidis itself. Compositions, kits and administration In a further aspect ofthe invention there are provided compositions comprising a BASB029 polynucleotide and/or a BASB029 polypeptide for administration to a cell or to a multicellular organism. The invention also relates to compositions comprising a polynucleotide and/or a polypeptide discussed herein or their agonists or antagonists. The polypeptides and polynucleotides of the invention may be employed in combination with a non-sterile or sterile carrier or carriers for use with cells, tissues or organisms, such as a pharmaceutical carrier suitable for administration to an individual. Such compositions comprise, for instance, a media additive or a therapeutically effective amount of a polypeptide and/or polynucleotide ofthe invention and a pharmaceutically acceptable carrier or excipient. Such carriers may include, but are not limited to, saline, buffered saline, dextrose, water, glycerol. ethanol and combinations thereof. The formulation should suit the mode of administration. The invention further relates to diagnostic and pharmaceutical packs and kits comprising one or more containers filled with one or more ofthe ingredients ofthe aforementioned compositions ofthe invention. Polypeptides, polynucleotides and other compounds ofthe invention may be employed alone or in conjunction with other compounds, such as therapeutic compounds. The pharmaceutical compositions may be administered in any effective, convenient manner including, for instance, administration by topical, oral, anal, vaginal, intravenous, intraperitoneal, intramuscular, subcutaneous, intranasal or intradermal routes among others. In therapy or as a prophylactic, the active agent may be administered to an individual as an injectable composition, for example as a sterile aqueous dispersion, preferably isotonic. In a further aspect, the present invention provides for pharmaceutical compositions comprising a therapeutically effective amount of a polypeptide and/or polynucleotide, such as the soluble form of a polypeptide and/or polynucleotide ofthe present invention, agonist or antagonist peptide or small molecule compound, in combination with a pharmaceutically acceptable carrier or excipient. Such carriers include, but are not limited to, saline, buffered saline, dextrose, water, glycerol, ethanol, and combinations thereof. The invention further relates to pharmaceutical packs and kits comprising one or more containers filled with one or more ofthe ingredients ofthe aforementioned compositions ofthe invention. Polypeptides, polynucleotides and other compounds ofthe present invention may be employed alone or in conjunction with other compounds, such as therapeutic compounds. The composition will be adapted to the route of administration, for instance by a systemic or an oral route. Preferred forms of systemic administration include injection, typically by intravenous injection. Other injection routes, such as subcutaneous, intramuscular, or intraperitoneal, can be used. Alternative means for systemic administration include transmucosal and transdermal administration using penetrants such as bile salts or fusidic acids or other detergents. In addition, if a polypeptide or other compounds ofthe present invention can be formulated in an enteric or an encapsulated formulation, oral administration may also be possible. Administration of these compounds may also be topical and/or localized, in the form of salves, pastes, gels, solutions, powders and the like. For administration to mammals, and particularly humans, it is expected that the daily dosage level ofthe active agent will be from 0.01 mg/kg to 10 mg/kg, typically around 1 mg/kg. The physician in any event will determine the actual dosage which will be most suitable for an individual and will vary with the age, weight and response ofthe particular individual. The above dosages are exemplary of the average case. There can, of course, be individual instances where higher or lower dosage ranges are merited, and such are within the scope of this invention. The dosage range required depends on the choice of peptide, the route of administration, the nature ofthe formulation, the nature ofthe subject's condition, and the judgment ofthe attending practitioner. Suitable dosages, however, are in the range of 0.1-100 μg/kg of subject. A vaccine composition is conveniently in injectable form. Conventional adjuvants may be employed to enhance the immune response. A suitable unit dose for vaccination is 0.5-5 microgram/kg of antigen, and such dose is preferably administered 1-3 times and with an interval of 1-3 weeks. With the indicated dose range, no adverse toxicological effects will be observed with the compounds ofthe invention which would preclude their administration to suitable individuals. Wide variations in the needed dosage, however, are to be expected in view ofthe variety of compounds available and the differing efficiencies of various routes of administration. For example, oral administration would be expected to require higher dosages than administration by intravenous injection. Variations in these dosage levels can be adjusted using standard empirical routines for optimization, as is well understood in the art. Sequence Databases, Sequences in a Tangible Medium, and Algorithms Polynucleotide and polypeptide sequences form a valuable information resource with which to determine their 2- and 3-dimensional structures as well as to identify further sequences of similar homology. These approaches are most easily facilitated by storing the sequence in a computer readable medium and then using the stored data in a known macromolecular structure program or to search a sequence database using well known searching tools, such as the GCG program package. Also provided by the invention are methods for the analysis of character sequences or strings, particularly genetic sequences or encoded protein sequences. Preferred methods of sequence analysis include, for example, methods of sequence homology analysis, such as identity and similarity analysis, DNA, RNA and protein structure analysis, sequence assembly, cladistic analysis, sequence motif analysis, open reading frame determination, nucleic acid base calling, codon usage analysis, nucleic acid base trimming, and sequencing chromatogram peak analysis. A computer based method is provided for performing homology identification. This method comprises the steps of: providing a first polynucleotide sequence comprising the sequence of a polynucleotide ofthe invention in a computer readable medium; and comparing said first polynucleotide sequence to at least one second polynucleotide or polypeptide sequence to identify homology. A computer based method is also provided for performing homology identification, said method comprising the steps of: providing a first polypeptide sequence comprising the sequence of a polypeptide ofthe invention in a computer readable medium; and comparing said first polypeptide sequence to at least one second polynucleotide or polypeptide sequence to identify homology. All publications and references, including but not limited to patents and patent applications, cited in this specification are herein incoφorated by reference in their entirety as if each individual publication or reference were specifically and individually indicated to be incoφorated by reference herein as being fully set forth. Any patent application to which this application claims priority is also incoφorated by reference herein in its entirety in the manner described above for publications and references. DEFINITIONS "Identity," as known in the art, is a relationship between two or more polypeptide sequences or two or more polynucleotide sequences, as the case may be. as determined by comparing the sequences. In the art, "identity" also means the degree of sequence relatedness between polypeptide or polynucleotide sequences, as the case may be, as determined by the match between strings of such sequences. "Identity" can be readily calculated by known methods, including but not limited to those described in (Computational Molecular Biology, Lesk, A.M., ed., Oxford University Press, New York, 1988; Biocomputing: Informatics and Genome Projects, Smith, D.W., ed., Academic Press, New York, 1993; Computer Analysis of Sequence Data, Part I, Griffin, A.M., and Griffin, H.G., eds., Humana Press, New Jersey, 1994; Sequence Analysis in Molecular Biology, von Heine. G., Academic Press, 1987: and Sequence Analysis Primer, Gribskov, M. and Devereux. J.. eds., M Stockton Press. New York, 1991; and Carillo, H., and Lipman, D., SIAM J. Applied Math, 48: 1073 (1988). Methods to determine identity are designed to give the largest match between the sequences tested. Moreover, methods to determine identity are codified in publicly available computer programs. Computer program methods to determine identity between two sequences include, but are not limited to, the GAP program in the GCG program package (Devereux, J.. et al.. Nucleic Acids Research 12(1): 387 (1984)), BLASTP. BLASTN (Altschul, S.F. et al., J. Molec. Biol. 215: 403-410 (1990), and FASTA( Pearson and Lipman Proc. Natl. Acad. Sci. USA 85; 2444-2448 (1988). The BLAST family of programs is publicly available from NCBI and other sources (BLAST Manual. Altschul, S., et al, NCBI NLM NIH Bethesda, MD 20894; Altschul, S., et al., J. Mol. Biol. 215: 403-410 (1990). The well known Smith Waterman algorithm may also be used to determine identity. Parameters for polypeptide sequence comparison include the following: Algorithm: Needleman and Wunsch, J. Mol Biol. 48: 443-453 (1970) Comparison matrix: BLOSSUM62 from Henikoff and Henikoff, Proc. Natl. Acad. Sci. USA. 89:10915-10919 (1992) Gap Penalty: 8 Gap Length Penalty: 2 A program useful with these parameters is publicly available as the "gap" program from Genetics Computer Group, Madison WI. The aforementioned parameters are the default parameters for peptide comparisons (along with no penalty for end gaps). Parameters for polynucleotide comparison include the following: Algorithm: Needleman and Wunsch, J. Mol Biol. 48: 443-453 (1970) Comparison matrix: matches = +10, mismatch = 0 Gap Penalty: 50 Gap Length Penalty: 3 Available as: The "gap" program from Genetics Computer Group, Madison WI. These are the default parameters for nucleic acid comparisons. A preferred meaning for "identity" for polynucleotides and polypeptides. as the case may be. are provided in (1) and (2) below. (1 ) Polynucleotide embodiments further include an isolated polynucleotide comprising a polynucleotide sequence having at least a 50, 60, 70, 80, 85, 90. 95, 97 or 100% identity to the reference sequence of SEQ ID NO:l, wherein said polynucleotide sequence may be identical to the reference sequence of SEQ ID NO: 1 or may include up to a certain integer number of nucleotide alterations as compared to the reference sequence, wherein said alterations are selected from the group consisting of at least one nucleotide deletion, substitution, including transition and transversion, or insertion, and wherein said alterations may occur at the 5' or 3' terminal positions ofthe reference nucleotide sequence or anywhere between those terminal positions, interspersed either individually among the nudeotides in the reference sequence or in one or more contiguous groups within the reference sequence, and wherein said number of nucleotide alterations is determined by multiplying the total number of nudeotides in SEQ ID NO:l by the integer defining the percent identity divided by 100 and then subtracting that product from said total number of nudeotides in SEQ ID NO:l, or: n n < x n - (x n • y), wherein n n is the number of nucleotide alterations, x n is the total number of nudeotides in SEQ ID NOT, y is 0.50 for 50%, 0.60 for 60%, 0.70 for 70%, 0.80 for 80%, 0.85 for 85%, 0.90 for 90%, 0.95 for 95%, 0.97 for 97% or 1.00 for 100%, and • is the symbol for the multiplication operator, and wherein any non-integer product of x n and y is rounded down to the nearest integer prior to subtracting it from x n . Alterations of a polynucleotide sequence encoding the polypeptide of SEQ ID NO:2 may create nonsense, missense or frameshift mutations in this coding sequence and thereby alter the polypeptide encoded by the polynucleotide following such alterations. By way of example, a polynucleotide sequence ofthe present invention may be identical to the reference sequence of SEQ ID NOT, that is it may be 100% identical, or it may include up to a certain integer number of nucleic acid alterations as compared to the reference sequence such that the percent identity is less than 100% identity. Such alterations are selected from the group consisting of at least one nucleic acid deletion, substitution, including transition and transversion, or insertion, and wherein said alterations may occur at the 5' or 3' terminal positions ofthe reference polynucleotide sequence or anywhere between those terminal positions, interspersed either individually among the nucleic acids in the reference sequence or in one or more contiguous groups within the reference sequence. The number of nucleic acid alterations for a given percent identity is determined by multiplying the total number of nucleic acids in SEQ ID NOT by the integer defining the percent identity divided by 100 and then subtracting that product from said total number of nucleic acids in SEQ ID NOT , or: n n < x n - (x n • y), wherein n n is the number of nucleic acid alterations, x n is the total number of nucleic acids in SEQ ID NO: 1 , y is, for instance 0.70 for 70%, 0.80 for 80%, 0.85 for 85% etc.. • is the symbol for the multiplication operator, and wherein any non-integer product of x n and y is rounded down to the nearest integer prior to subtracting it from x n . (2) Polypeptide embodiments further include an isolated polypeptide comprising a polypeptide having at least a 50, 60, 70, 80, 85, 90, 95, 97 or 100% identity to a polypeptide reference sequence of SEQ ID NO:2, wherein said polypeptide sequence may be identical to the reference sequence of SEQ ID NO:2 or may include up to a certain integer number of amino acid alterations as compared to the reference sequence, wherein said alterations are selected from the group consisting of at least one amino acid deletion, substitution, including conservative and non-conservative substitution, or insertion, and wherein said alterations may occur at the amino- or carboxy-terminal positions ofthe reference polypeptide sequence or anywhere between those terminal positions, interspersed either individually among the amino acids in the reference sequence or in one or more contiguous groups within the reference sequence, and wherein said number of amino acid alterations is determined by multiplying the total number of amino acids in SEQ ID NO:2 by the integer defining the percent identity divided by 100 and then subtracting that product from said total number of amino acids in SEQ ID NO:2, or: n a < x a - (x a • y), wherein n a is the number of amino acid alterations, x a is the total number of amino acids in SEQ ID NO:2, y is 0.50 for 50%, 0.60 for 60%, 0.70 for 70%, 0.80 for 80%, 0.85 for 85%. 0.90 for 90%, 0.95 for 95%, 0.97 for 97% or 1.00 for 100%, and • is the symbol for the multiplication operator, and wherein any non-integer product of x a and y is rounded down to the nearest integer prior to subtracting it from x a . By way of example, a polypeptide sequence ofthe present invention may be identical to the reference sequence of SEQ ID NO:2, that is it may be 100% identical, or it may include up to a certain integer number of amino acid alterations as compared to the reference sequence such that the percent identity is less than 100% identity. Such alterations are selected from the group consisting of at least one amino acid deletion, substitution, including conservative and non-conservative substitution, or insertion, and wherein said alterations may occur at the amino- or carboxy-terminal positions ofthe reference polypeptide sequence or anywhere between those terminal positions, interspersed either individually among the amino acids in the reference sequence or in one or more contiguous groups within the reference sequence. The number of amino acid alterations for a given % identity is determined by multiplying the total number of amino acids in SEQ ID NO:2 by the integer defining the percent identity divided by 100 and then subtracting that product from said total number of amino acids in SEQ ID NO:2. or: n a ≤ x a " ( x a * v )> wherein n a is the number of amino acid alterations, x a is the total number of amino acids in SEQ ID NO:2, y is, for instance 0.70 for 70%, 0.80 for 80%, 0.85 for 85% etc., and • is the symbol for the multiplication operator, and wherein any non-integer product of x a and y is rounded down to the nearest integer prior to subtracting it from x a . "Individual(s)," when used herein with reference to an organism, means a multicellular eukaryote, including, but not limited to a metazoan, a mammal, an ovid, a bovid, a simian, a primate, and a human. "Isolated" means altered "by the hand of man" from its natural state, i.e., if it occurs in nature, it has been changed or removed from its original environment, or both. For example, a polynucleotide or a polypeptide naturally present in a living organism is not "isolated." but the same polynucleotide or polypeptide separated from the coexisting materials of its natural state is "isolated", as the term is employed herein. Moreover, a polynucleotide or polypeptide that is introduced into an organism by transformation, genetic manipulation or by any other recombinant method is "isolated" even if it is still present in said organism, which organism may be living or non-living. "Polynucleotide(s)" generally refers to any polyribonucleotide or polydeoxyribonucleotide, which may be unmodified RNA or DNA or modified RNA or DNA including single and double-stranded regions. "'Variant" refers to a polynucleotide or polypeptide that differs from a reference polynucleotide or polypeptide, but retains essential properties. A typical variant of a polynucleotide differs in nucleotide sequence from another, reference polynucleotide. Changes in the nucleotide sequence ofthe variant may or may not alter the amino acid sequence of a polypeptide encoded by the reference polynucleotide. Nucleotide changes may result in amino acid substitutions, additions, deletions, fusions and truncations in the polypeptide encoded by the reference sequence, as discussed below. A typical variant of a polypeptide differs in amino acid sequence from another, reference polypeptide. Generally, differences are limited so that the sequences ofthe reference polypeptide and the variant are closely similar overall and, in many regions, identical. A variant and reference polypeptide may differ in amino acid sequence by one or more substitutions, additions, deletions in any combination. A substituted or inserted amino acid residue may or may not be one encoded by the genetic code. A variant of a polynucleotide or polypeptide may be a naturally occurring such as an allelic variant, or it may be a variant that is not known to occur naturally. Non-naturally occurring variants of polynucleotides and polypeptides may be made by mutagenesis techniques or by direct synthesis. "Disease(s)" means any disease caused by or related to infection by a bacteria, including , for example, upper respiratory tract infection, invasive bacterial diseases, such as bacteremia and meningitis. EXAMPLES: The examples below are carried out using standard techniques, which are well known and routine to those of skill in the art, except where otherwise described in detail. The examples are illustrative, but do not limit the invention. Examplel : Discovery and confirmatory DNA sequencing of the BASB029 gene from two N. meningitidis strains. A: BASB029 in N. meningitidis serogroup B strain ATCC 13090. The BASB029 gene of SEQ ID NOT was first discovered in the Incyte PathoSeq database containing unfinished genomic DNA sequences ofthe N meningitidis strain ATCC 13090. The translation of the BASB029 polynucleotide sequence, showed in SEQ ID ΝO:2, showed significant similarity (52 % identity in a 582 amino acids overlap) to the Haemophilus inβuenzae surface fibril (HSF) protein. The sequence ofthe BASB029 gene was further confirmed experimentally. For this puφose, genomic DNA was extracted from 10'° cells ofthe N. meningitidis cells (strain ATCC 13090) using the QIAGEN genomic DNA extraction kit (Qiagen Gmbh), and lμg of this material was submitted to Polymerase Chain Reaction DNA amplification using primers Hsfl (5-- GGG GCA TAT GAA CAA AAT ATA CCG CAT CAT TTG GAA-3') [SEQ ID NO:5] containing an internal Ndel site (underlined) and Hsf2 (5'- GGG GCT CGA GCC ACT GAT AAC CGA CAG ATG CGG A-3') [SEQ ID NO:6] containing an internal Xhol site (underlined). This PCR product was gel-purified and subjected to DNA sequencing using the Big Dye Cycle Sequencing kit (Perkin-Elmer) and an ABI 373 A/PRISM DNA sequencer. DNA sequencing was performed on both strands with a redundancy of 2 and the full-length sequence was assembled using the SeqMan program from the DNASTAR Lasergene software package The resulting DNA sequence turned out to be 100 % identical to SEQ ID NOT. B: BASB029 in N. meningitidis serogroup B strain H44/76. The sequence ofthe BASB029 gene was also determined in another N meningitidis serogroup B strain, the strain H44/76. For this puφose, genomic DΝA was extracted from the N meningitidis strain H44/76 using the experimental conditions presented in Example 1. This material (1 μg) was then submitted to Polymerase Chain Reaction DΝA amplification using primers Hsfl and Hsf2 specific for the BASB029 gene. A 4389bp DΝA fragment was obtained, digested by the Ndel/Xhol restriction endonucleases and inserted into the corresponding sites ofthe pET-24b cloning/expression vector (Novagen) using standard molecular biology techniques (Molecular Cloning,a Laboratory Manual, Second Edition, Eds: Sambrook, Fritsch & Maniatis, Cold Spring Harbor press 1989). Recombinant pET-24b/BASB029 was then submitted to DNA sequencing using the Big Dyes kit (Applied biosystems) and analyzed on a ABI 373/A DNA sequencer in the conditions described by the supplier. As a result, the polynucleotide and deduced polypeptide sequences, referred to as SEQ ID NO:3 and SEQ ID NO:4 respectively, were obtained. Using the PILEUP program from the GCG package, an alignment ofthe polynucleotide sequences of SEQ ID NOT and 3 was performed, and is displayed in Figure 1 ; their level of identity amounts to 96.8 % as determined by the GAP program. Using the same PILEUP program, an alignment of the polypeptide sequences of SEQ ID NO:2 and 4 was performed, and is displayed in Figure 2; their level of identity amounts to 94.2 %, as determined by the GAP program. Taken together, these data indicate strong sequence conservation ofthe BASB029 gene among the two N. meningitidis serogroup B strains. Example 2: Expression and purification of recombinant BASB029 protein in Escherichia colL The construction ofthe pET-24b/BASB029 cloning/expression vector was described in Example IB. This vector harbours the BASB029 gene isolated from the strain H44/76 in fusion with a stretch of 6 Histidine residues, placed under the control ofthe strong bacteriophage T7 gene 10 promoter. For expression study, this vector was introduced into the Escherichia coli strain Novablue (DE3) (Novagen), in which, the gene for the T7 polymerase is placed under the control ofthe isopropyl-beta-D thiogalactoside (IPTG)- regulatable lac promoter. Liquid cultures (100 ml) ofthe Novablue (DE3) [pET- 24b/BASB029] E. coli recombinant strain were grown at 37°C under agitation until the optical density at 600nm (OD600) reached 0.6. At that time-point, IPTG was added at a final concentration of ImM and the culture was grown for 4 additional hours. The culture was then centrifuged at 10,000 rpm and the pellet was frozen at -20°C for at least 10 hours. After thawing, the pellet was resuspended during 30 min at 25°C in buffer A (6M guanidine hydrochloride, 0.1M NaH2PO4, 0.01M Tris, pH 8.0), passed three-times through a needle and clarified by centrifugation (20000φm, 15 min). The sample was then loaded at a flow-rate of lml/min on a Ni2+ -loaded Hitrap column (Pharmacia Biotech). After passsage ofthe flowthrough, the column was washed succesively with 40ml of buffer B (8M Urea, 0TMNaH2PO4, 0.01M Tris, pH 8.0), 40ml of buffer C (8M Urea. 0.1MNaH2PO4, 0.01M Tris, pH 6.3). The recombinant protein BASB029/His6 was then eluted from the column with 30ml of buffer D (8M Urea, 0.1MNaH2PO4, 0.01M Tris. pH 6.3) containing 500mM of imidazole and 3ml-size fractions were collected. As shown in Figure 3 (lanel), a highly enriched (Purity estimated to more than 90% pure in coomassie staining) BASB029/His6 protein, migrating at 66kDa (estimated relative molecular mass), was eluted from the column. This polypeptide was reactive against a mouse monoclonal antibody raised against the 5-histidine motif (see figure 3, lane2). Taken together, these data indicate that the BASB029 gene can be expressed and purified under a recombinant form (BASB029/His6) in E.coli. Example 3 : Immunization of mice with recombinant BASB029 Partially purified recombinant BASB029 protein expressed in E. coli has been injected three times in Balb/C mice on days 0, 14 and 29 (8 animals/group). Animals were injected by the subcutaneous route with around 5μg of antigen in two different formulations : either adsorbed on lOOμg AlPO 4 or formulated in SBAS2 emulsion (SB62 emulsion containing 5μg MPL and 5μg QS21 per dose). A negative control group consisting of mice immunized with the SB AS2 emulsion only has also been added in the experiment. Mice were bled on days 29 (15 days Post II) and 35 (6 days Post III) in order to detect specific anti-BASB029 antibodies. Specific anti-BASB029 antibodies were measured on pooled sera (from 10 mice/group) by western-blotting on six different NmB strains (Figures 4 and 5). Recognition ofthe BASB029 epitopes on different NmB strains by western-blotting In this test, immunized mice sera (pooled) have been tested by western-blotting for recognition of the B ASB029 epitopes on six different neisseria meningitidis B strains : H44/76 (B:15:P1.7, 16, lineage ET-5), M97 250987 (B:4:P1.15), BZ10 (B:2b:P1.2. lineage A4), BZ198 (B:NT*: -, lineage 3), EG328 (B:NT*, lineage ST- 18), and the ATCC 13090 Men B strain, as well as on partially purified recombinant BASB029 protein (* : NT : Not Typed). Briefly. lOμl (> 10 8 cells/lane) of each sample treated with sample buffer (10 min at 95 °C) are put into a SDS-PAGE gradient gel (Tris-glycine 4-20%, Novex, code n°EC60252). Electrophoretic migration occurs at 125 volts for 90 min. Afterwards. proteins are transfeπed to nitrocellulose sheet (0.45μm, Bio-rad code n° 162-0114) at 100 volts for 1 hour using a Bio-rad Trans-blot system (code n° 170-3930). Filter was blocked with PBS - 0.05% Tween 20 overnight at room temperature, before incubation with the mice sera containing the anti-BASB029 antibodies coming from both AlPO 4 and SBAS2 formulations. These sera are diluted 100 times in PBS - 0.05% Tween 20, and incubated on the nitrocellulose sheet for two hours at room temperature with gentle shaking, using a mini-blotter system (Miniprotean, Bio-rad code n° 170-4017). After three repeated washing steps in PBS - 0.05% Tween 20 for 5 min., the nitrocellulose sheet is incubated at room temperature for lhour under gentle shaking with the appropriate conjugate (biotinylated anti-mouse Ig antibodies from sheep, Amersham code n°RPN1001) diluted at 1/500 in the same washing buffer. The membrane is washed three times as previously, and incubated for 30 min with agitation using the streptavidin-peroxidase complex (Amersham code n°1051) diluted at 1/1000 in the washing buffer. After the last three repeated washing steps, the revelation occurs during the 20 min incubation time in a 50 ml solution containing 30 mg 4-chloro-l-naphtol (Sigma), 10ml methanol, 40 ml PBS, and 30μl of H 2 O 2 . The staining is stopped while washing the membrane several times in distillated water. Results illustrated in Figures 4 and 5 show that all strains tested present the expected band around 65-70 kDa, and two other major bands around 55 and 90 kD, which are clearly related to this BASB029 protein (polymers, degradation products). This means that the B ASB029 protein is probably expressed in most of, if not all, the NmB strains. In Figure 4, the recombinant BASB029 protein appears as four different proteins, two at the expected molecular weights of 65-70 Kda, and two others at very molecular heights (> 200 kDa) which are probably aggregates of recombinant BASB029 protein. Example 4: Presence of anti-BASB029 antibodies in sera from convalescent patients. In this test, convalescent sera have been tested by western-blotting for recognition ofthe purified recombinant BASB029 protein. Briefly, 5μg of partially purified BASB029 NmB protein are put into a SDS-PAGE gradient gel (4-20%, Novex, code n°EC60252) for electrophoretic migration. Proteins are transfeπed to nitrocellulose sheet (0.45μm, Bio-rad code n° 162-0114) at 100 volts for 1 hour using a Bio-rad Trans-blot system (code n°l 70-3930). Afterwards, filter is blocked with PBS - 0.05 % Tween 20 overnight at room temperature, before incubation with the human sera. These sera are diluted 100 times in PBS - 0.05% Tween 20. and incubated on the nitrocellulose sheet for two hours at room temperature with gentle shaking, using a mini-blotter system (Miniprotean, Bio-rad code n° 170-4017). After three repeated washing steps in PBS - 0.05% Tween 20 for 5 min., the nitrocellulose sheet is incubated at room temperature for lhour under gentle shaking with the appropriate conjugate (biotinylated anti-human Ig antibodies, from sheep, Amersham code n°RPN1003) diluted at 1/500 in the same washing buffer. The membrane is washed three times as previously, and incubated for 30 min with agitation using the streptavidin-peroxidase complex (Amersham code n°1051) diluted at 1/1000 in the washing buffer. After the last three repeated washing steps, the revelation occurs during the 20 min incubation time in a 50ml solution containing 30 mg 4-chloro-l-naphtol (Sigma), 10ml methanol, 40ml of ultra-pure water, and 30μl of H 2 O 2 . The staining is stopped while washing the membrane several times in distillated water. Results are illustrated in Figures 6 and 7 which show that all 7 convalescents react against the rec. BASB029 protein at around 65 - 70 kDa, while the two upper bands (> 200 kDa) are also recognized by most of them, while with a weak reaction for few of them. Reactivities against the high MW proteins confirms that these two bands are clearly related to the BASB029 protein, which are probably under their aggregated forms. In part A of the Figures 6 and 7, the same reactions against these four bands are seen with the immunized mice sera. This clearly confirms that all these four bands are related to the recombinant BASB029 protein. Negative mice sera don't react with the recombinant protein as shown also in Fig 7. Deposited materials A deposit containing a Neisseria meningitidis Serogroup B strain has been deposited with the American Type Culture Collection (herein "ATCC") on June 22, 1997 and assigned deposit number 13090. The deposit was described as Neisseria meningitidis (Albrecht and Ghon) and is a freeze-dried, 1.5-2.9 kb insert library constructed from N. meningitidis isolate. The deposit is described in Int. Bull. Bacteriol. Νomencl. Taxon. 8: 1-15 (1958). The Neisseria meningitidis strain deposit is refeπed to herein as "the deposited strain" or as "the DΝA of the deposited strain. " The deposited strain contains the full length BASB029 gene. The sequence of the polynucleotides contained in the deposited strain, as well as the amino acid sequence of any polypeptide encoded thereby, are controlling in the event of any conflict with any description of sequences herein. The deposit ofthe deposited strain has been made under the terms ofthe Budapest Treaty on the International Recognition of the Deposit of Micro-organisms for Puφoses of Patent Procedure. The strain will be iπevocably and without restriction or condition released to the public upon the issuance of a patent. The deposited strain is provided merely as convenience to those of skill in the art and is not an admission that a deposit is required for enablement, such as that required under 35 U.S.C. §112. INDICATIONS RELATING TO DEPOSITED MICROORGANISM OR OTHER BIOLOGICAL MATERIAL (PCT Rule \2bιs) The indications made below relate to the deposited microorganism or other biological material referred to in the description B. IDENTIFICATIONS OF DEPOSIT Further deposits are identified on an additional sheet [ | Name of depositary institution AMERICAN TYPE CULTURE COLLECTION Address of depositary institution (including postal code and country) 10801 UNIVERSITY BLVD, MANASSAS , VIRGINIA 20110-2209 , UNITED STATES OF AMERICA Date of deposit Accession Number 22 June 1997 ( 22/06/97) 13090 C. ADDITIONAL INDICATIONS (leave blank if not applicable) This information is continued on an additional sheet [_] In respect of those designations where a European Patent is sought, a sample of the deposited microorganism will be made available until the publication of the mention of the grant of the European Patent or until the date on which the application has been refused or withdrawn, only by issue of such a sample to an expert nominated by the person requesting the sample. D. DESIGNATED STATES FOR WHICH INDICATIONS ARE MADE (if the indications are not for all designated States) E. SEPARATE FURNISHING OF INDICATIONS (leave blank if not applicable) The indications listed below will be submitted to the International Bureau later (specify the general nature of ' the indications e g . "Accession Number of Deposit") For receiving Office use only For International Bureau use only I j This sheet was received with the international application D This sheet was received by the International Bureau on. Authorized officer Authorized officer Form PCT/RO/134 (JuM 998

Claims: CLAIMS: 1. An isolated polypeptide comprising an amino acid sequence which has at least 85% identity to the amino acid sequence selected from the group consisting of: SEQ ID NO:2, 5 SEQ ID NO:4. 2. An isolated polypeptide as claimed in claim 1 in which the amino acid sequence has at least 95% identity to the amino acid sequence selected from the group consisting of: SEQ ID NO:2, SEQ ID NO:4. [ 0 3. The polypeptide as claimed in claim 1 comprising the amino acid sequence selected from the group consisting of: SEQ ID NO:2, SEQ ID NO:4. 4. An isolated polypeptide of SEQ ID NO:2, SEQ ID NO:4. 5 5. An immunogenic fragment ofthe polypeptide as claimed in any one of claims 1 to 4 in which the immunogenic activity of said immunogenic fragment is substantially the same as the polypeptide of SEQ ID NO:2, SEQ ID NO:4. 0 6. An isolated polynucleotide comprising a nucleotide sequence encoding a polypeptide that has at least 85% identity to the amino acid sequence of SEQ ID NO:2, 4 over the entire length of SEQ ID NO:2, 4 respectively; or a nucleotide sequence complementary to said isolated polynucleotide. 5 7. An isolated polynucleotide comprising a nucleotide sequence that has at least 85% identity to a nucleotide sequence encoding a polypeptide of SEQ ID NO:2, 4 over the entire coding region; or a nucleotide sequence complementary to said isolated polynucleotide. 8. An isolated polynucleotide which comprises a nucleotide sequence which has at least 85% identity to that of SEQ ID NO: 1 , 3 over the entire length of SEQ ID NO: 1 , 3 respectively; or a nucleotide sequence complementary to said isolated polynucleotide. 9. The isolated polynucleotide as claimed in any one of claims 6 to 8 in which the identity is at least 95% to SEQ ID NOT, 3. 10. An isolated polynucleotide comprising a nucleotide sequence encoding the polypeptide of SEQ ID NO:2, SEQ ID NO:4. 1 1. An isolated polynucleotide comprising the polynucleotide of SEQ ID NOT, SEQ ID NO:3. 12. An isolated polynucleotide comprising a nucleotide sequence encoding the polypeptide of SEQ ID NO:2, SEQ ID NO:4 obtainable by screening an appropriate library under stringent hybridization conditions with a labeled probe having the sequence of SEQ ID NO: 1 , SEQ ID NO: 3 or a fragment thereof. 13. An expression vector or a recombinant live microorganism comprising an isolated polynucleotide according to any one of claims 6 - 12. 14. A host cell comprising the expression vector of claim 13 or a subcellular fraction or a membrane of said host cell expressing an isolated polypeptide comprising an amino acid sequence that has at least 85% identity to the amino acid sequence selected from the group consisting of: SEQ ID NO:2, SEQ ID NO:4. 15. A process for producing a polypeptide comprising an amino acid sequence that has at least 85%o identity to the amino acid sequence selected from the group consisting of: SEQ ID NO:2, SEQ ID NO:4 comprising culturing a host cell of claim 14 under conditions sufficient for the production of said polypeptide and recovering the polypeptide from the culture medium. 16. A process for expressing a polynucleotide of any one of claims 6 - 12 comprising transforming a host cell with the expression vector comprising at least one of said polynucleotides and culturing said host cell under conditions sufficient for expression of any one of said polynucleotides. 17. A vaccine composition comprising an effective amount ofthe polypeptide of any one of claims 1 to 5 and a pharmaceutically acceptable caπier. 18. A vaccine composition comprising an effective amount ofthe polynucleotide of any one of claims 6 to 12 and a pharmaceutically effective caπier. 19. The vaccine composition according to either one of claims 17 or 18 wherein said composition comprises at least one other Neisseria meningitidis antigen. 20. An antibody immunospecific for the polypeptide or immunological fragment as claimed in any one of claims 1 to 5. 21. A method of diagnosing a Neisseria meningitidis infection, comprising identifying a polypeptide as claimed in any one of claims 1 - 5, or an antibody that is immunospecific for said polypeptide, present within a biological sample from an animal suspected of having such an infection. 22. Use of a composition comprising an immunologically effective amount of a polypeptide as claimed in any one of claims 1 - 5 in the preparation of a medicament for use in generating an immune response in an animal. 23. Use of a composition comprising an immunologically effective amount of a polynucleotide as claimed in any one of claims 6 - 12 in the preparation of a medicament for use in generating an immune response in an animal. 24. A therapeutic composition useful in treating humans with Neisseria meningitidis disease comprising at least one antibody directed against the polypeptide of claims 1 - 5 and a suitable pharmaceutical caπier.

Inventors: RUELLE JEAN-LOUIS, RUELLE, JEAN-LOUIS
Applicants: RUELLE JEAN LOUIS, SMITHKLINE BEECHAM BIOLOG, RUELLE, JEAN-LOUIS, SMITHKLINE BEECHAM BIOLOGICALS S.A.
Application year: 1999
Main classification: C12N15
IPC codes: A61K39, A61K48, A61P31, C07K14, C07K16, C12N1, C12N5, C12N15, C12P21, C12Q1, C12R1, G01N33