Process for S-aryl-L-cysteine and derivatives

Abstract: The present invention provides methods for preparing S-aryl cysteines in enantiomeric excess of greater than about 96%. Specifically, the present invention provides enantioselective methods for preparing S-aryl cysteines starting from cystine, cysteine or a serine.

Description: S-aryl-L-cysteines are useful intermediates in the synthesis of a variety of pharmaceutically active compounds. Chiral S-aryl-L-cysteines have been used successfully to target the human immunodeficient virus (HIV) and are used in the treatment of AIDS (Kaldor et al., J. Med. Chem. 1997, 40 (24), 3979-3985). Representative potent and tight binding inhibitors of the human immunodeficient virus protease which contain an arylthio group are shown in Figure 1 . Many currently available synthetic methods for S-aryl-L-cysteines involve the preparation of racemic mixtures. There are, however, a number of disadvantages associated with racemic mixtures of such compound. A racemic mixture of an S-aryl-L-cysteine results in production of racemic drugs. It is well known that certain physiological properties of chiral drugs are dependent upon stereochemistry of the drug and the undesired side-effects are often attributed to the presence of the undesired stereoisomer of the chiral drug. Accordingly, a high enantioselective synthesis of a chiral drug will result in a drug having a desired therapeutic activity with a reduced amount of undesired side-effects. Of course, the synthesis of a chiral drug can include a step of separating a racemic mixture; however, this is often time consuming and costly. In addition, racemic synthesis requires discarding one half of the compound unless the undesired isomer can be converted to a desired isomer. Moreover, not all racemic compounds can be resolved to provide a satisfactory yield of a desired enantiomer. Current methods for enantioselective synthesis of S-aryl-L-cysteines involve enzymatic methods (see, e.g., USP 5.756.319 or European Patent Application No. 754,759, which are assigned to Mitsui Toatsu Chemicals, Inc.), applicable, however, to the preparation of only a limited number of S-aryl-L-cysteines. Most of the current chemical synthetic methods for enantioselective preparation of S-aryl-L-cysteines result in a racemic mixture, use elaborate reagents dramatically increasing the overall cost, or result in too low levels of enantioselectivity to be useful in a pharmaceutical process. For example Hickman et al., Australian Journal of Chemistry, 1985, Vol. 38, No.6, p.899-904 disclose a preparation of aromatic S-substituted N-acetyl-L-cysteines by conversion of N-acetyl-L-cysteine with an arylhalide in the presence of coopper iodide. However, the yield achieved re.g. for N-acetyl-S-(2-thienyl)-cysteine is with 28 % very low. Recently, D.W. Knight and A.W. Sibley ( J. Chem. Soc., Perkin Trans. 1, 1997, 2179-2187) reported that reaction of methyl (S)-2-benzyloxycarbonylamino-3-methylsulfonyloxy-propanoate with freshly prepared sodium thiophenylate in DMF at about 0°C afforded the desired N-carbobenzyloxy-S-phenyl-L-cysteine methyl ester (or methyl (R)-2-benzyloxycarbonylamino-3-phenylthiopropanoate) in 98% yield providing a reported optical rotation of [α] 20 D - 17.2 ( c , 1.8; MeOH). No enantiomeric ratio of the product was reported. Furthermore, the use of sodium phenolate, prepared from sodium hydride, thiophenol and DMF is not amenable to large scale manufacture. Therefore, there is a need for an efficient, concise and enantioselective method suitable for the large scale manufacture of S-aryl-L-cysteines using relatively inexpensive reagents. Definitions "Alkyl" comprises straight or branched chain groups having 1 to about 10 carbon atoms. Alkyl groups optionally can be substituted with one or more substituents, such as halogen, aryl, hydroxy, alkoxy, carboxy, oxo and cycloalkyl. There may be optionally inserted along the alkyl group one or more oxygen, sulfur or substituted or unsubstituted nitrogen atoms. Exemplary alkyl groups include methyl, ethyl, i -propyl, n -butyl, t -butyl, n -pentyl, heptyl, benzyl, and octyl. "Aryl" means an aromatic group which has at least one ring which has a conjugated pi electron system and includes, without limitation, carbocyclic aryl, heterocyclic aryl, biaryl groups and heterocyclic biaryl, all of which can be optionally substituted. "Heterocyclic aryl groups" refer to groups having at least one heterocyclic aromatic ring containing from 1 to 3 heteroatoms in the ring with the remainder being carbon atoms. Suitable heteroatoms include, without limitation, oxygen, sulfur, and nitrogen. Exemplary heterocyclic aryl groups include furanyl, thienyl, pyridyl, pyrrolyl, N-alkyl pyrrolo, pyrimidyl, pyrazinyl, imidazolyl, benzofuranyl, quinolinyl, and indolyl. Preferably, the aryl group is substituted or unsubstituted aryl selected from the group consisting of phenyl, naphthyl, pyridyl, furyl, thiophenyl, pyrazyl and pyrrolyl. More preferably, the aryl group is substituted or unsubstituted aryl group selected from the group consisting of phenyl, naphthyl and pyridyl, still more preferably the aryl group is selected from the group consisting of substituted and unsubstituted phenyl, and most preferably the aryl group is phenyl. The term "S-aryl" refers to a substituent wherein an aromatic group is bonded to a sulfur atom. "S-aryl" group may also be referred to as an "aryl thioether" group. The term "S-arylation" refers to the process of substituting a compound or groups with an S-aryl group. The term "metal" comprises alkali metals, alkaline-earth metals, transition metals, noble metals, platinum metals, rare metals, rare-earth metals, actinide metals, light metals, and heavy metals. Examples of such metals are aluminum, iron, copper, cobalt, potassium, sodium, tin and zinc. The term "catalyst" refers to any substance of which a fractional percentage notably affects the rate of a chemical reaction without itself being consumed or undergoing a chemical change, as defined in "Hawley's Condensed Chemical Dictionary", 11th ed., revised by N. Irving Sax and Richard J. Lewis, Sr., Van Nostrand Reinhold Company, New York, p. 748 (1987). The term "stoichiometric" refers to the use or addition of an equivalent mole ratio or amount of a reagent relative to a selected substrate, molecule, or compound in a reaction. The term "chiral" has the usual meaning known to a person skilled in the art. The term "enantiomeric excess" refers to a difference between the amount of one enantiomer and the amount of the other enantiomer that is present in the product mixture. Thus for example, enantiomeric excess of 96% refers to a product mixture having 98% of one enantiomer and 2% of the other enantiomer. The following abbreviations and terms are used herein: "CBZ" means benzyloxycarbonyl or carbobenzyloxy. "DMF" means dimethylformamide. "Tosylate" or "Tos" means p -toluenesulfonate ester. "Mesylate" or "Ms" means methanesulfonate ester. The present invention is directed to a method for preparing S-aryl-L-cysteines (which are useful intermediates in a variety of pharmaceutically active compounds) in high enantiomeric excess. Unless the context requires otherwise, reference to any compound is to be considered as a reference to an individual enantiomer of the compound, and to racemic or non-racemic mixtures thereof. BRIEF DESCRIPTION OF FIGURES 1-3: Figure 1 shows representative compounds of HIV protease inhibitors which contain a moiety derived from S-aryl-L-cysteine. Figure 2 illustrates one embodiment of the present invention for preparing an S-aryl-L-cysteine compound from cystine. Figure 3 illustrates another embodiment of the present invention for preparing an S-aryl-L-cysteine compound which utilizes cysteine. As shown in Figure 2, one embodiment of the present invention provides a method for producing S-L-aryl cysteine by contacting cystine with copper optionally in combination with copper (I) and/ or (II) bromide as coupling agent and contacting the resulting compound with an aryl halide for a time and under conditions effective to produce S-aryl-L- cysteine. When cystine is used as a starting material the amount of aryl halide used is preferably from about 1 equiv. to about 6 equiv., more preferably from about 2 equiv. to about 6 equiv., still more preferably from about 2 equiv. to about 4 equiv., and most preferably about 3 equiv. Preferably halide is selected from the group consisting of iodide, bromide and chloride, more preferably halide is selected from the group consisting of bromide and iodide, and most preferably halide is bromide. Contacting cystine with Copper results in cleavage of the disulfide bond generating a copper thiolate of cysteine which undergoes a coupling reaction to form the desired product and copper halide. The temperature of the reaction is preferably from about 80° to 150°C, more preferably from about 100 to 130°C, and most preferably from about 115°C to about 125°C. The reaction time can vary depending upon the identity of the copper and/or the aryl halide, but generally it has been found that the reaction time of at least about 1 hour produces the desired product in a relatively high yield, preferably at least about 15 hours, and more preferably at least about 18 hours. As shown in Figure 3, another embodiment of the present invention provides a method for producing S-aryl-L-cysteine by contacting cysteine with an aryl halide in the presence of copper oxide. When cysteine is used as a starting material in a coupling reaction, the amount of aryl halide used is preferably from about 1 equiv. to about 5 equiv., more preferably from about 1.1 equiv. to about 4 equiv., still more preferably from about 1.2 equiv. to about 3 equiv., and most preferably about 1.5 equiv. It has been found that the production of S-aryl-L-cysteine by this method is facilitated by heating the mixture. Preferably, the reaction temperature is from about 80° to 150°C, more preferably from about 100 to 130°C, and most preferably from about 115°C to about 125°C. A variety of aryl halides can be reacted with the thiolate to produce the S-aryl-L-cysteine compound. Preferably, the aryl halide is phenyl bromide. A method of the present invention for producing S-aryl-L-cysteine includes the presence of a coupling agent which generates from cystine or cysteine the reactive thiolate compound of the formula wherein M is copper, P 1 is hydrogen or an amino protecting group and P 2 is hydrogen or carboxylic protecting group. It will be appreciated that the above structure merely represents an idealized representation of thiolate. The exact structure of the thiolate can be a dimer, trimer or other polymeric form of the reactive intermediate, e.g., with copper binding more than one thiolate group. Moreover, copper can also bind other ligands such as solvent molecules or other species which may be present in the reaction mixture. For the conversion of cystin the coupling agent copper (I) or (II) bromide is added preferably, from about 0 mol% to about 100 mol%., relative to aryl halide, is added, more preferably from about 0.2 mol% to about 5 mol%, and most preferably about 1 mol% to about 3 mol%. Typically about 6 mol% of copper (I) or copper (II) bromide is added. It should be recognized that other functional groups present in cystine or cysteine can be protected or unprotected. It has been found that after the reaction, the copper (I) or copper (II) bromide or the resulting product thereof may be isolated and recycled to be used in another coupling reaction. In this manner, the cost of coupling agent and the disposal cost of the resulting coupling agent product, e.g., copper (I) bromide, can be substantially reduced. When cystine is used as starting material bromine may further be added as oxidizing agent.. Although the method of the present invention can be conducted in the absence of a solvent, it has been found that the presence of a relatively high boiling point solvent provides a reaction medium which can be heated to a desired temperature. Thus, the solvent has boiling point higher than the desired reaction temperature. Preferably the solvent is selected from the group consisting of acetonitrile, glymes, dimethylacetamide, dimethylformamide, dimethylsulfoxide, diethylacetamide, dimethylbutyramide and N-methyl-2-pyrrolidone, and more preferably the solvent is dimethylformamide. The reaction time can vary depending upon the identity of the metal oxide and/or the aryl halide, but generally it has been found that the reaction time of at least about 1 hour produces a relatively high yield of the desired product with high enantioselectivity, preferably at least about 15 hours, and more preferably at least about 18 hours Typically, the thiolate is generated in situ and is used without further purification. The above methods of the present invention can include protecting the amino group of the amino acid (cystine or cysteine ). Any of the known amino protecting groups can be used. Examples of some protecting groups are described in "Solid Phase Peptide Synthesis" by G. Barany and R. B. Merrifield in Peptides, Vol. 2, edited by E. Gross and J. Meienhoffer, Academic Press, New York, N.Y., pp. 100-118 (1980), and "Protective Groups in Organic Synthesis" by Green, T., John Wiley & Sons, Inc., New York, NY., 1981, pp. 218-287. Exemplary N-amino protecting groups include acetyl, formyl, benzoyl, substituted benzoyls, FMOC, Bspoc, Bsmoc, t-butyloxycarbonyl (BOC), t-amyloxycarbonyl (Mcb), 2-(p-biphenylyl)-propyl-2-oxycarbonyl (Bpoc), benzyloxycarbonyl (or carbobenzyloxy, CBZ), phthaloyl, piperidino-oxycarbonyl, trifluoroacetyl and the like. Other N-amino protecting groups include optionally protected α-amino acids which are linked with the carboxyl moiety of the α-amino acids. Preferably the amino protecting group is metyl carbamate or CBZ. Amino protecting group can be removed under various conditions, including mild acidic or basic conditions. The preferred protecting groups are those which can be cleaved by an acid or a base, or reductive conditions. For example, the amino group can be protected by contacting the amino acid with benzyl chloroformate in the presence of a base. Any base that can neutralize the acidic proton that is formed by the reaction of benzyl chloroformate and the amino group can be used. Exemplary bases useful in protection of the amino group include carbonates such as sodium carbonate, potassium carbonate and lithium carbonate; bicarbonates such as sodium bicarbonate, potassium bicarbonate and lithium bicarbonate; hydroxides such as sodium hydroxide, lithium hydroxide, calcium hydroxide, magnesium hydroxide and potassium hydroxide; and sterically hindered amines such as triethyl amine and diisopropyl ethyl amine. Preferably, the base is selected from the group consisting of carbonates, bicarbonates and hydroxides, more preferably the base is selected from the group consisting of carbonates and bicarbonates, and most preferably the base is a bicarbonate. In one particular embodiment of the present invention, the amino group is protected by contacting the amino acid with benzyl chloroformate in the presence of sodium bicarbonate to provide N-benzyloxycarbonyl protected amino acid. For the protection of the carboxy group of the amino acid any of the known carboxy protecting groups can be used. Protection of the carboxy moiety of amino acids are described in "The Peptides," E. Gross and J. Meienhofer, Eds., Vol.3, Academic Press, NY (1981), pp. 101-135, and "Protective Groups in Organic Synthesis" by Green, T., John Wiley & Sons, Inc., New York, NY., 1981, pp. 152-192. Exemplary carboxy protecting groups include esters such as alkyl esters including methyl, ethyl, tert-butyl, methoxymethyl, 2,2,2-trichloroethyl and 2-haloethyl; benzyl esters such as triphenylmethyl, diphenylmethyl, p -bromobenzyl, o -nitrobenzyl and the like; silyl esters such as trimethylsilyl, triethylsilyl, t -butyldimethylsilyl and the like; amides and hydrazides. Other carboxy protecting groups can include optionally protected α-amino acids which are linked with the amino moiety of the α-amino acids. Preferably the carboxylic acid protecting group is an ester, more preferably the carboxylic acid protecting group is an alkyl ester, and most preferably the carboxylic acid protecting group is selected from the group consisting of methyl ester and ethyl ester. In a particular embodiment of the present invention, the carboxylic acid of the amino acid is protected as methyl ester by contacting the amino acid with thionyl chloride in the presence of methanol. Alternatively, the carboxylic acid is protected as methyl ester by contacting the amino acid with gaseous hydrochloric acid in the presence of methanol. The above method can also include protecting both the amino group and the carboxy group of the amino acid. It will be appreciated that the amino and carboxy groups in the amino acid can be protected in any sequence. The S-aryl-L-cysteine reaction product of the present invention which is recovered from the reaction mixture can be further purified by distillation or crystallization. For instance, a reaction product obtained in liquid form, is dissolved in toluene and crystallized from a relatively non-polar recrystallization solvent to afford a product of higher purity. Preferably, the non-polar recrystallization solvent is selected from the group consisting of hexane, ethyl acetate, toluene, xylene, benzene, pentane, ethers and mixtures thereof. The S-aryl-L-cysteine can be recovered as a salt. For example, adding an acid such as hydrochloric acid; or an organic acid including tartaric acid, acetic acid, and/or citric acid to S-aryl-L-cysteine can result in the formation of the corresponding S-aryl-L-cysteine salt which can be easily isolated. Alternatively, the free carboxy group can react with a base to generate a carboxylate salt which can form a solid. In yet another alternative, the presence of both free amino and carboxy groups results in the formation of a zwitter ion which can precipitate as a solid. The present invention is further illustrated by the following examples EXAMPLE 1 Preparation of N,N'-bis-benzyloxycarbonyl cystine dimethyl ester using thionyl chloride and cystine. In a 500 ml jacketed round bottomed flask is placed cystine (20 g, 83.2 mmol) and methanol (250 ml). The reaction mixture is cooled to 0-5°C and thionyl chloride (12.7 ml, 0.175 mol) is added while maintaining the temperature at less than 10°C. At the end of the addition, the reaction is heated to reflux for 4 hours and excess methanol is removed by distillation. As the product begins to precipitate, water was added followed by a slow addition of sodium bicarbonate (29.4 g, 0.35 mole) to the solution at less than 5°C. After the bicarbonate is added, benzyl chloroformate (25 ml, 0.175 mole) is slowly added. The reaction mixture is maintained at <5°C for 1 hour and slowly warmed to room temperature. The reaction mixture is heated to 30 to 40°C and the organic phase is separated and the aqueous phase is washed with toluene (3 x 20 ml). The combined organic solution is washed once with sodium bicarbonate (25 ml), followed by 5% HCl, and saturated NaCI, dried over MgSO 4 , and concentrated in vacuo by rotoevaporation to afford 44.25 g of an oil (0.0825 mole, 99.1% yield). A portion of this oil (31.55 g) was recrystallized from EtOAc-hexane to give a colorless solid (24.95 g, 79.1 % yield). mp 70-72°C ; assayed as 97.5 % by A/N HPLC. EXAMPLE 2 Preparation of N,N'-bis-benzyloxycarbonyl cystine dimethyl ester using MeOH/HCl and cystine. To a cold (-5 to -10°C) solution of methanol (100 ml) was bubbled HCl gas. About 11.0 g of HCl gas was absorbed into the solution. To a slurry of cystine in 100 ml methanol was added the above prepared HCl/methanol solution. The mixture was heated to reflux. After about one hour, a clear solution was obtained. The mixture was heated to reflux for a total of 4 hours, cooled to room temperature and transferred to a one neck round bottomed flask. Concentration of the mixture by rotoevaporation afforded white solids. The white solids were suspended in 400 ml of toluene. A solution of NaHCO 3 (31.5 g) in 300 ml of water was added to give a clear two phase solution. Benzyl chloroformate (25.0 ml, 0.175 mole) was added dropwise at 16-18°C. The reaction mixture was then stirred at 16-18°C for 4 hours. The aqueous phase was separated and extracted with toluene (3 x 50 ml). The combined toluene extracts were washed with water, dilute sodium carbonate solution, 5% HCl solution, and saturated sodium chloride solution. The organic phase was dried over MgSO 4 and concentrated in vacuo to give an oil. The oil was dissolved in ethyl acetate (100 ml). Hexane (175 ml) was added and the mixture was seeded. The solution was stirred at room temperature overnight. The product was filtered, washed with a hexane:EtOAc (9:1, 100 ml) mixture and dried under vacuum at 45°C to afford the product in 87.4% yield, 39.0 g, assayed as 98.5% by A/N HPLC. EXAMPLE 3 Preparation of N-CBZ S-phenyl-L-cysteine methyl ester using crude N,N'-bis-benzyloxycarbonyl cystine dimethyl ester. Into a 250 ml round bottomed flask was added N,N'-bis-benzyloxycarbonyl cystine dimethyl ester (12.7 g, 237 mmol), copper powder (3.08 g) and dimethylformamide (130 ml). The stirred mixture was heated to 70°C. Bromobenzene, i.e ., phenyl bromide, (10 ml, 95 mmol) was charged to an addition funnel and added dropwise to the reaction mixture at about 70-80°C over 30 minutes. The reaction mixture was kept at 75-80°C for 35 minutes, warmed to 90°C for 25 minutes, heated to 100°C for 4 hours, and then at 110°C for another 48 hours. The reaction was monitored by thin layer chromatography. The reaction mixture was cooled to 50°C and DMF was distilled off under reduced pressure at 50-60°C where 80 ml of distillate was recovered. The reaction mixture was diluted with toluene (150 ml) and water (50 ml). The resulting mixture was heated to reflux for 30 minutes and the solution was quickly filtered over a pad of celatom. The celatom pad was washed with excess toluene and the mixture was diluted with water. The phases were separated and the toluene phase was washed with water, 10% HCl/water (100 ml, vol/vol), and saturated sodium chloride. The resulting solution was concentrated by rotoevaporation to afford a light yellow oil. The oil was dissolved in toluene (15 ml) and seeded to induce crystallization. Hexane (15 ml) was added followed by another 60 ml of hexane, and the slurry was stirred overnight at room temperature. The product was filtered, washed with excess hexane, and air dried to afford a colorless solid (80.7% yield; mp 62-64 °C). EXAMPLE 4 Preparation of N-CBZ S-phenyl-L-cysteine methyl ester using purified N,N'-bis-benzyloxycarbonyl cystine dimethyl ester. Copper powder (4.62 g), bromobenzene (15.0 ml, 142 mmol), and DMF (55 ml) was charged to a 250 ml 3-neck round bottomed flask. The resulting mixture was heated to 110°C using an external oil bath. To the stirred mixture was added a solution of purified N-CBZ cystine methyl ester (19.05 g, 35.5 mmol, 97.5% by assay) in DMF (40 ml) over 2 hours and 30 minutes. The resulting mixture was stirred at 110°C for 18 hours and then at 130 ± 2°C for about 24 hours. The resulting mixture was cooled to 65°C and DMF was distilled off under reduced pressure (50-60°C) until a thick slurry was formed. The mixture was diluted with toluene (150 ml) and heated to 70 to 75°C for 15-20 minutes. The solution was quickly filtered over a pad of celatom and the celatom pad was washed with warm (70°C) toluene. The toluene phase was washed with water (2 x 100 ml), 10% aqueous HCl (1 x 100 ml), water (1 x 100 ml) and saturated sodium chloride (1 x 100 ml). The reddish brown solution was dried over anhydrous magnesium sulfate. The solution was filtered through a pad of Filtrol-13 (an acid activated clay from the Filtrol Corp.) to afford yellow solids. The filtrol pad was washed with toluene (100 ml). Toluene was distilled under reduced pressure at 40-45°C until an oil was obtained. The oil was dissolved in toluene (25 ml), hexane (25 ml) was added and the mixture was seeded to precipitate out the desired product. Hexane (200 ml) was added and the resulting mixture was stirred at room temperature for 2.5 days. The product was filtered, washed with excess hexane and dried under vacuum at 45°C to afford the desired product in 66.9% yield (16.4 g, assayed as 98.3% by A/N HPLC (area normalized high performance liquid chromatography)). Concentration of the mother liquors afforded 6.2 grams of an oil (41% product by A/N assay). EXAMPLE 5 Preparation of N-CBZ S-phenyl-L-cysteine methyl ester from N-CBZ cysteine methyl ester. A round bottomed flask was charged with CBZ-cysteine methyl ester (5.4 g, 20.0 mmol), copper oxide (2.8 g), and bromobenzene (4.2 ml, 39.9 mmol) in dimethylformamide (25.0 ml). The mixture was heated to reflux (145 ± 2°C) for 19 hours. Analysis by HPLC revealed 25.9% N-CBZ S-phenyl-L-cysteine methyl ester and 6.0% N-CBZ S-benzyl cysteine methyl ester. EXAMPLE 6 Preparation of N-CBZ S-phenyl-L-cysteine from N,N'-bis-benzyloxycarbonyl cystine. A mixture of N,N'-bis-benzyloxycarbonyl cystine (3.81 g, 7.5 mmol), copper powder (0.95 g, 15.0 mmol), and bromobenzene (3.32 ml, 4.95g, 31.5 mmol) in dimethylformamide (20 ml) was heated to 120°C for 19 hours. The resulting mixture was cooled to 80°C and dimethylformamide was removed by vacuum distillation at 80-95°C, where 15 ml of distillate was collected. The resulting residue was diluted with toluene (70 ml) and stirred for 1 hour at 70-75°C. The product was filtered and washed with excess toluene. The combined organic phases were washed with 10% aqueous HCl (1 x 70 ml), water (2 x 70 ml), saturated sodium chloride (1 x 70 ml), and the solution was dried over anhydrous magnesium sulfate. The solution was filtered, the cake was washed with excess toluene, and the organic solution was concentrated via rotoevaporation to afford 4.8 g of a light brown oil which solidified upon standing. 96.6% yield, 4.8 g, assayed as 96.33% by A/N HPLC, containing 3.67% of the corresponding S-benzyl acid. EXAMPLE -7 preparation of N-CBZ S-(3-methoxyphenyl)-L-cysteine methyl ester from N,N'-bis-benzyloxycarbonyl cystine dimethyl ester and 3-bromoanisole. Starting material, N,N'-bis-benzyloxycarbonyl cystine dimethyl ester (3.0 g, 5.6 mmol) was dissolved in anhydrous DMF at a solvent ratio of 10:1. To the solution were added 2.0 equivalents of copper powder (0.71g, 11.2 mmol) and 4.0 equivalents of 3-bromoanisole (4.1 g, 22.3 mmol), and the mixture was heated to 120-130 °C. After stirring overnight the reaction mixture was checked by HPLC and was determined to have <5% starting material. The reaction mixture was cooled and the DMF as well as most of the excess 3-bromoanisole was removed under reduced pressure. The crude mixture was dissolved with 10 volumes of toluene and the copper and copper salts were removed by filtration. The filtrate was washed once with 30 ml of a 50:50 mixture (10% NH 4 OH, 15% w/w NH 4 Cl) followed by one wash with saturated brine. The organic layer was concentrated under reduced pressure and the crude product was purified by silica gel chromatography resulting in a light yellow oil; yield 2.73g ( 65%). EXAMPLE 8 Preparation of N-CBZ S-(2-(6-methoxynaphthalenyl))-L-cysteine methyl ester from N,N'-bis-benzyloxycarbonyl cystine dimethyl ester and 2-bromo-6-methoxynaphthalene. N,N'-Bis-benzyloxycarbonyl cystine dimethyl ester (2.75 g, 5.12 mmol) was dissolved in anhydrous DMF at a solvent ratio of 10:1. To the solution were added 2.0 equivalents of copper powder (0.65 g, 10.2 mmol) and 4.37 equivalents of 2-bromo-6-methoxynaphthalene (5.3 g, 22.4 mmol), and the mixture was heated to 120-130°C. After stirring overnight the reaction mixture was checked for completion by HPLC and was determined to have <5% starting material. The reaction mixture was cooled and the DMF was removed under reduced pressure. The crude mixture was dissolved with 10 volumes of toluene and the copper and copper salts were removed by filtration. The filtrate was washed once with 30 ml of a 50:50 mixture (10% NH 4 OH, 15% w/w NH 4 Cl) followed by one wash with saturated brine. The organic layer was concentrated under reduced pressure and the crude product was purified by silica gel chromatography resulting in a light brown oil 1.96 g (45%). EXAMPLE 9 Preparation of N-CBZ S-(3-pyridyl)-L-cysteine methyl ester from N,N'-bis-benzyloxycarbonyl cystine dimethyl ester and 3-bromopyridine. N,N'-bis-benzyloxycarbonyl cystine dimethyl ester (3.0 g, 5.6 mmol) was dissolved in anhydrous DMF at a solvent ratio of 10:1. To the solution were added 2.0 equivalents of copper powder (0.71g, 11.2 mmol) and 4.0 equivalents of 3-bromopyridine (3.5 g, 22.3 mmol), and the mixture was heated to 120-130 °C. After stirring overnight the reaction mixture was checked with HPLC and was determined to have <5% starting material. The reaction mixture was cooled and the DMF was removed under reduced pressure. The crude mixture was dissolved with 10 volumes of toluene and the copper and copper salts were removed by filtration. The filtrate was washed once with 30 ml of a 50:50 mixture (10% NH 4 OH, 15% w/w NH 4 Cl) followed by one wash with saturated brine. The organic layer was concentrated under reduced pressure and the crude product was purified by silica gel chromatography resulting in a reddish-brown oil 2.05 g (53%). EXAMPLE 10 Preparation of N,N'-bis-(3-acetoxy-2-methylbenzoyl)-cystine dimethyl ester from cystine dimethyl ester hydrochloride and 3-acetoxy-2-methylbenzoyl chloride. Cystine dimethyl ester hydrochloride (5.36 g, 0.0157 mole), 3-acetoxy-2-methylbenzoyl chloride (6.67 g, 0.0314 mole), and triethyl amine (6.35 g, 0.0628 mole) were dissolved in dichloromethane (50 ml) and the resulting mixture was stirred at ambient temperature overnight. The organic phase was washed with 2 N hydrochloric acid (2 x 20 ml) and then with water (2 x 20 ml). The organic layer was dried over MgSO 4 and concentrated on a rotary evaporator to give a light yellow solid. Trituration of the solid with 100 ml of toluene gave a light yellow crystalline solid, which was isolated by filtration and dried at 50°C (under vacuum) to give N,N'-bis-(3-acetoxy-2-methylbenzoyl)-cystine dimethyl ester (4.30 g, yield 44.1%). A second crop was obtained by silica gel column chromatography of the mother liquors, eluting with 50% methylene chloride in ethyl acetate. EXAMPLE 11 Preparation of N-(3-acetoxy-2-methylbenzoyl)-S-phenyl-L-cysteine methyl ester from N,N'-bis-(3-acetoxy-2-methylbenzoyl)-cystine dimethyl ester and bromobenzene. Into a 3-necked 50 ml round bottomed flask equipped with an overhead stirrer under nitrogen atmosphere was added N,N'-bis-(3-acetoxy-2-methylbenzoyl)-cystine dimethyl ester (4.3 g, 0.00693 g), bromobenzene (6.5 g, 4.4 ml, 0.0416 mole), copper (0.92 g, 0.0145 mole), and dimethyl formamide (25 ml). The resulting mixture was heated to 120 ± 3°C for 19 hours. Volatiles were removed by distillation at 80-85°C on a rotary evaporator under reduced pressure to afford a brown residue. The residue was dissolved in 30 ml of dichloromethane and washed with 3 N hydrochloric acid (20 ml), followed by washing with water (2 x 20 ml). The organic phase was dried over MgSO 4 , filtered, and concentrated on a rotary evaporator to afford a light yellow solid (3.6 g, yield 53%). EXAMPLE 12 Preparation of N-CBZ S-phenyl-L-cysteine methyl ester from N,N'-Bis-benzyloxycarbonyl cystine dimethyl ester using copper powder and catalytic CuBr 2 . N,N'-Bis-benzyloxycarbonyl cystine dimethyl ester (84.00 g, 7.45 mmol), copper powder (4.72 g, 74 mmol), cupric bromide (CuBr 2 , 1.84 g, 8.23 mmol), phenyl bromide (2.4 ml, 22.7 mmol), and dimethylformamide (24 ml) were combined in a 50 ml round bottomed flask. The resulting stirred mixture was immersed in a preheated oil bath at 140°C for 3 hours. HPLC analysis showed 1.5% starting material remaining. Dimethylformamide was removed by distillation at 120°C and 50 mmHg. The residue was diluted with 15 ml of toluene and filtered through a pad of celatom (diatomaceous earth) to remove the solids. The filtrate was washed twice with 30 ml of 1 M HCl and once with 30 ml of water. Toluene was removed on a rotary evaporator under reduced pressure. The residue was crystallized by dissolving in approximately 5 ml of toluene and adding hexane (50 ml) slowly at 40°C to initiate precipitation. The mixture was cooled to ambient temperature, filtered and the product was washed with 20 ml of hexane. The solid was dried under vacuum at 50°C to give 3.05 g of an off-white solid (yield 59%). m.p. 61.9-63.5°C. EXAMPLE 13 Preparation of N-CBZ S-phenyl-L-cysteine methyl ester from N,N'-bis-benzyloxycarbonyl cystine dimethyl ester using copper powder and catalytic CuBr 2 . To a 25 ml 2 neck round bottomed flask purged with nitrogen and equipped with condenser were added N,N'-bis-benzyloxycarbonyl cystine dimethyl ester (2.0 g, 3.73 mmol), copper powder (0.5g, 7.87 mmol), dimethylformamide (10 ml), and phenyl bromide (2.0 ml, 1.34 g, 8.54 mmol). The resulting stirred mixture was heated to 115 - 125°C for 22 hours. Two drops of bromine were added to the reaction and the resulting mixture was heated for another 22 hours where no starting material remained. HPLC analysis revealed 88.4% of the desired product, N-CBZ S-phenyl-L-cysteine methyl ester. EXAMPLE 14 Preparation of N-CBZ S-phenyl-L-cysteine methyl ester from N,N'-bis-benzyloxycarbonyl cystine dimethyl ester. Into a 250 ml round bottomed flask equipped with a thermometer, mechanical stirrer and nitrogen inlet were added N,N'-Bis-benzyloxycarbonyl cystine dimethyl ester (13.25 g, 0.025 mole), copper powder (4.0 g, 0.0625 mole), and copper (II) bromide (0.34 g, 0.00152 mole). To the mixture was added bromobenzene (31.6 ml, 0.3 mole) and dimethyl sulfoxide (67 ml, dried over 4A sieve). The stirred content was heated to 130°C for 1 hour and 15 minutes where no starting material remained. Analysis by HPLC showed 94.3% N-CBZ S-phenyl-L-cysteine methyl ester and no starting material. The resulting mixture was cooled to 120°C for another 18 hours and analyzed for reaction completion. HPLC analysis showed 81.7% N-CBZ S-phenyl-L-cysteine methyl ester, 3.9% benzyl alcohol, 4.2% diphenyl sulfide, and 3.4% N-CBZ S-phenyl-L-cysteine benzyl ester. EXAMPLE 15 Preparation of N-CBZ S-phenyl-L-cysteine methyl ester from N,N'-bis-benzyloxycarbonyl cystine dimethyl ester. Into a 250 ml round bottomed flask equipped with a thermometer, mechanical stirrer and nitrogen inlet were added N,N'-Bis-benzyloxycarbonyl cystine dimethyl ester (6.7 g, 0.013 mole), copper powder (2.0 g, 0.0315 mole), and copper (II) bromide (0.2g, 0.895 mmole). To the mixture was added bromobenzene (15.8 ml, 23.56 g, 0.15 mole) and dimethyl sulfoxide (33 ml, dried over 4A sieve). The stirred content was heated to 100°C for 18 hours and sampled for reaction completion. Analysis by HPLC showed 88.8% N-CBZ S-phenyl-L-cysteine methyl ester and no starting material. The resulting mixture was cooled to room temperature and analyzed for reaction completion. HPLC analysis showed 88.8% N-CBZ S-phenyl-L-cysteine methyl ester, 2.5% benzyl alcohol, 0.5% methyl carbamate, 2.1% diphenyl sulfide, and 1.2% diphenyl disulfide.

Claims: A method for preparing an S-aryl-L-cysteine comprising contacting either cystine, optionally in protected form, with copper optionally in combination with copper (I) bromide and/or copper (II) bromide or cysteine, optionally in protected form, with copper oxide to form a thiolate of the formula wherein M is a copper; P 1 is H or an amino protecting group; and P 2 is H or a carboxylic acid protecting group and contacting the thiolate so formed with an aryl halide. The method of Claim 1, wherein P 1 is an amino protecting group. The method of Claim 2, wherein P 1 is carbobenzyloxy. The method of any one of Claims 1-3, wherein P 2 is a carboxy protecting group. The method of Claim 4, wherein P 2 is methyl. The method of any one of Claims 1-5, wherein said aryl halide is phenyl bromide. The method of any one of Claims 1-6 further comprising a reaction solvent selected from the group consisting of dimethylacetamide, dimethyl formamide, dimethyl sulfoxid, diethylacetamide, dimethylbutyramide, and N-methyl-2-pyrrolidone. The method of Claim1, wherein bromine is present as oxidizing agent when cystine is selected as starting compound.

Inventors: BROWN JACK D, DAUER RICHARD R, HARRINGTON PETER JOHN, JOHNSTON DAVE A, KHATRI HIRALAL N, ROWE GARY K, TOPPING ROBERT J, BROWN, JACK D., DAUER, RICHARD R., HARRINGTON, PETER JOHN, JOHNSTON, DAVE A., KHATRI, HIRALAL N., ROWE, GARY K., TOPPING, ROBERT J.
Applicants: HOFFMANN LA ROCHE, F. HOFFMANN-LA ROCHE AG
Application year: 1999
Main classification: C07C323
IPC codes: C07C319, B01J31, C07B61, C07C323, C07C321